Detection of circulating immune complexes with polyethylene glycol precipitation F(ab')2 anti-C3 ELISA.
A polyethylene glycol (PEG) precipitation F(ab')2 anti-C3 ELISA for the detection of complement-fixing IgG circulating immune complexes (CIC) is described. For this assay, test sera were treated with 3.5% PEG and then measured with F(ab')2 anti-C3 ELISA. The lower detection limit was 4 micrograms/ml of heat aggregated human IgG (HAHG). Intra-assay coefficient of variation (CV) was 4.9-8.3%. High levels of CIC are found in the sera of patients with systemic lupus erythematosus (SLE), hepatitis B and stomach cancer.
Xu
R J
RJ
Department of Clinical Biochemistry, 97th Hospital of PLA, Xuzhou, China.
Gu
Y A
YA
D000936:Antigen-Antibody Complex / Q000032:analysis*; D011232:Chemical Precipitation; D003176:Complement C3 / Q000276:immunology*; D003168:Complement Fixation Tests; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006509:Hepatitis B / Q000276:immunology; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D011092:Polyethylene Glycols; D015203:Reproducibility of Results; D013274:Stomach Neoplasms / Q000276:immunology
D000936:Antigen-Antibody Complex; D003176:Complement C3; D007140:Immunoglobulin Fab Fragments; D011092:Polyethylene Glycols