title: Role of adenosine in regulation of regional cerebral blood flow in sensory cortex.
abstract: We have previously demonstrated that rat pial arterioles located on the somatosensory cortex dilated in response to contralateral sciatic nerve stimulation (SNS). We hypothesized that the vasodilation was mediated by adenosine, released as a result of somatosensory cortex activation. To test this hypothesis, we examined the effects of SNS (0.15-0.2 V, 5 ms, 5 Hz for 20 s) on pial arterioles under conditions of altered adenosine availability. Cerebrospinal fluid (CSF) adenosine was altered by perfusing mock CSF, under a cranial window in anesthetized rats, containing either an adenosine uptake competitor (dipyridamole or inosine) or an adenosine receptor blocker (theophylline). With CSF only, SNS caused pial arterioles (resting diam, 29 +/- 1 micron) to dilate by 38 +/- 10% (peak magnitude) for 32 +/- 2 s. Dipyridamole (10(-6) M) significantly (P less than 0.02) enhanced both the magnitude (to 62 +/- 12%) and duration (to 68 +/- 10 s) of the response. Similarly, inosine (10(-3) M) significantly (P less than 0.02) potentiated the vasodilative response from resting values of 27 +/- 5% and 34.8 +/- 4.1 s to 37 +/- 6% and 89.6 +/- 14.1 s. In contrast, theophylline (5 x 10(-5) M) significantly (P less than 0.001) attenuated arteriolar vasodilation from resting values of 38 +/- 5% and 29.3 +/- 1.2 s to 18 +/- 3% and 22.0 +/- 0.9 s. Neither dipyridamole nor theophylline had a significant effect on neuronal response (sensory-evoked response) recorded from the somatosensory cortex. These results suggest that adenosine is involved in the regulation of pial vasodilation during cerebral cortical activation.
authors: Department of Neurological Surgery, University of Washington, Seattle 98104.
authors: K R
authors: KR
authors: Ko
authors: A C
authors: AC
authors: Ngai
authors: H R
authors: HR
authors: Winn
mesh_terms: D000241:Adenosine / Q000502:physiology*; D000287:Administration, Topical; D000818:Animals; D001160:Arterioles / Q000187:drug effects; D002560:Cerebrovascular Circulation / Q000502:physiology*; D004176:Dipyridamole / Q000494:pharmacology; D004558:Electric Stimulation; D005073:Evoked Potentials, Somatosensory / Q000187:drug effects; D007288:Inosine / Q000494:pharmacology; D008297:Male; D010841:Pia Mater / Q000098:blood supply; D051381:Rats; D011919:Rats, Inbred Strains; D012584:Sciatic Nerve; D013003:Somatosensory Cortex / Q000098:blood supply*; D013806:Theophylline / Q000494:pharmacology; D014664:Vasodilation / Q000187:drug effects
chemical_list: D007288:Inosine; D004176:Dipyridamole; D013806:Theophylline; D000241:Adenosine