Crystallization and preliminary X-ray studies of psophocarpin B1, a chymotrypsin inhibitor from winged bean seeds.
Psophocarpin B1 is a 20,000 Mr protein of winged bean (Psophocarpus tetragonolobus) seeds having chymotrypsin inhibitory activity. Single crystals of this protein suitable for X-ray crystallographic studies have been obtained by the vapour diffusion method using ammonium sulphate. The crystals are hexagonal, space group P6(4)22 or P6(2)22, cell dimensions a = b = 61 A, c = 210 A. They are stable to irradiation with X-rays and diffract to at least 2.6 A resolution.
Dattagupta
J K
JK
Crystallography and Molecular Biology Division, Saha Institute of Nuclear Physics, Bidhan Nagar, Calcutta, India.
Chakrabarti
C
C
Podder
A
A
Dutta
S K
SK
Singh
M
M
D002848:Chromatography, DEAE-Cellulose; D002918:Chymotrypsin / Q000037:antagonists & inhibitors*; D003460:Crystallization; D007887:Fabaceae; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification*; D010946:Plants, Medicinal; D011487:Protein Conformation; D014961:X-Ray Diffraction
D010940:Plant Proteins; D002918:Chymotrypsin