title: Study of the effect of a glycosaminoglycan-peptide complex on the degradative enzyme activities in human osteoarthritic cartilage.
abstract: The in vitro collagenolytic and proteoglycanasic activity from human fibrillated osteoarthritic cartilage was determined using labelled proteoglycans and type II collagen as substrates. In vitro, a glycosaminoglycan-peptide complex (GP-C Rumalon) induced a dose-dependent inhibition of both collagenolytic and proteoglycanasic activities while sodium salicylate and indomethacin had only a weak suppressive effect on proteoglycanase. Phospholipase A2 activity was unmodified by GP-C suggesting that the effect of the drug on degradative enzymes was unrelated to prostaglandin formation.
authors: Laboratoire d'Exploration Articulaire, Hôpital Edouard Herriot, Lyon, France.
authors: E
authors: E
authors: Vignon
authors: A
authors: A
authors: Martin
authors: P
authors: P
authors: Mathieu
authors: T
authors: T
authors: Conrozier
authors: P
authors: P
authors: Louisot
authors: M
authors: M
authors: Richard
mesh_terms: D000368:Aged; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002358:Cartilage, Articular / Q000201:enzymology*; D003094:Collagen / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D010450:Endopeptidases / Q000378:metabolism; D005260:Female; D005270:Femur Head / Q000201:enzymology; D006801:Humans; D008297:Male; D008666:Metalloendopeptidases; D008875:Middle Aged; D010003:Osteoarthritis / Q000201:enzymology*; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D014020:Tissue Extracts / Q000494:pharmacology*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D014020:Tissue Extracts; C000562:Rumalon; D003094:Collagen; D010741:Phospholipases A; D054467:Phospholipases A2; D010450:Endopeptidases; C022474:proteoglycan-degrading metalloendopeptidases; D008666:Metalloendopeptidases