A protocol for the purification of bovine serum albumin free of deoxyribonuclease activity.
Bovine serum albumin, free of deoxyribonuclease activity, was obtained in our laboratory using ion-exchange chromatography followed by acetylation. Chromatography on four different resins (DEAE-52, P-11, hydroxylapatite and Q Sepharose fast-flow) was examined. Fractions from Q Sepharose chromatography, eluted with a linear gradient 0-1.0 M NaCl and subsequently acetylated, proved to be the most effective method for obtaining deoxyribonuclease-free bovine serum albumin.
Trujillo
L E
LE
Center for Genetic Engineering and Biotechnology, Havana, Cuba.
Castellanos
L
L
Garcia
O
O
Herrera
L
L
D000107:Acetylation; D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange / Q000379:methods*; D003851:Deoxyribonucleases / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000302:isolation & purification*
D012710:Serum Albumin, Bovine; D003851:Deoxyribonucleases