[Effect of preincubation time and concentration of selective media on the effectiveness of salmonella organism isolation from frozen meat homogenates].
Double concentrated RV and SC media were used for selective multiplication of frozen meat homogenates preincubated in buffered peptone water during 6 and 20 hours. It was found that cultures of 2 ml of homogenate preincubated during 6 hours in 10 ml of twofold concentrated RV medium, and selective multiplication during 20 hours made possible obtaining of a greater number of Salmonella isolates as compared to samples preincubated during 20 hours and transferred in volumes of 0.1 ml into 10 ml of normal concentration medium. On the other hand, the growth of 1 ml of a culture preincubated during 6 hours transferred into 10 ml of twofold concentrated SC medium yielded a similar number of salmonella isolates as in the case of samples preincubated during 24 h and transferred in 1 ml volumes into 10 ml of the SC medium without concentration change.
Pogorzelska
E
E
Zakładu Zywienia Klinicznego Instytutu Zywności i Zywienia w Warszawie.
Kafel
S
S
Uradziński
J
J
D003470:Culture Media; D005516:Food Microbiology; D005519:Food Preservation; D005615:Freezing; D008461:Meat Products; D012475:Salmonella / Q000302:isolation & purification*; D013997:Time Factors
D003470:Culture Media
Wpływ czasu przednamnazania oraz stezenia pozywek wybiórczo-namnazajacych na efektywność izolacji pałeczek Salmonlla z homogenizatów miesa mrozonego.