[Comparative analysis of the structure of SUP2 genes in Pichia pinus and Saccharomyces cerevisiae].
SUP2(SUP35) is an omnipotent suppressor gene, coding for an EF-1 alpha-like protein factor, involved in the control of translational accuracy in yeast Saccharomyces cerevisiae. A SUP2 gene analogue from yeast Pichia pinus was isolated by complementation of temperature-sensitive sup2 mutation of S. cerevisiae. Nucleotide sequence of the SUP2 gene of P. pinus codes for a protein of 82.4 kDa exceeding the SUP2 protein of S. cerevisiae for 6 kDa. The SUP2 gene product of P. pinus is similar to the Sup2 protein of S. cerevisiae by its structure and includes a highly conservative (76%) C-terminal region homologus to EF-1 alpha and a lowly conservative N-terminal region. The relation between the evolutionary conservativity of different regions of the Sup2 protein and their functional significance is discussed.
Kushnirov
V V
VV
Ter-Avanesian
M D
MD
Smirnov
V N
VN
Chernov
Iu O
IuO
Derkach
I L
IL
Novikova
O N
ON
Inge-Vechtomov
S G
SG
Neĭstat
M A
MA
Tolstorukov
I I
II
D000595:Amino Acid Sequence; D001483:Base Sequence; D003062:Codon; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010843:Pichia / Q000235:genetics*; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013489:Suppression, Genetic
D003062:Codon; D005656:Fungal Proteins
Sravnitel'nyĭ analiz struktury genov SUP2 drozhzheĭ Pichia pinus i Saccharomyces cerevisiae.