title: Automation of specific human gene detection.
abstract: An instrument/chemistry system is described that automates a new chemical procedure functionally equivalent to Southern blotting. A fluorescence gel scanner that detects migrating DNA fragments in real-time analyzes the samples produced by a prototype liquid-handling instrument that automates a solution-phase hybridization/solid-phase capture chemistry for DNA analysis. The combination of this chemistry, the gel scanner, and robotic automation eliminates the tedium encountered in traditional manual methods for specific gene detection and reduces analysis time from days to hours. Restriction fragment lengths are measured with high precision by comparison with in-lane standards to minimize effects attributable to migration anomalies. The utility of this automated system is demonstrated by executing a clinical research application involving hybridization to a multi-copy repeat sequence on the Y chromosome and its detection.
authors: Applied Biosystems, Inc., Foster City, CA 94404.
authors: P E
authors: PE
authors: Mayrand
authors: L B
authors: LB
authors: Hoff
authors: L J
authors: LJ
authors: McBride
authors: J A
authors: JA
authors: Bridgham
authors: R
authors: R
authors: Cathcart
authors: K P
authors: KP
authors: Corcoran
authors: G S
authors: GS
authors: Golda
authors: D H
authors: DH
authors: Keith
authors: E W
authors: EW
authors: Lachenmeier
authors: D E
authors: DE
authors: Madden
mesh_terms: D001331:Automation; D001483:Base Sequence; D015139:Blotting, Southern / Q000379:methods*; D002460:Cell Line; D004247:DNA / Q000032:analysis*; D005260:Female; D015894:Genome, Human; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data
chemical_list: D004247:DNA