Interaction of puromycin with acceptor site of human placenta 80 S ribosomes.
The complex N-AcPhe-tRNA(Phe).poly(U).80 S ribosome from human placenta was treated with puromycin taken in various concentrations. Based on the kinetic data of N-acetylphenylalanyl-puromycin formation, the association constant of puromycin with the acceptor site of the ribosome was estimated to be (3.96 +/- 0.84) x 10(4) M-1 at 37 degrees C.
Graifer
D M
DM
Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences, Novosibirsk.
Fedorova
O S
OS
Karpova
G G
GG
D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D010920:Placenta; D011691:Puromycin / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*
D011691:Puromycin