title: Increased rate of base substitution in a hamster mutator strain obtained during serial selection for gene amplification.
abstract: The pattern of mutations produced by a mutator gene (obtained during serial selection for amplification of the dihydrofolate reductase [dhfr] locus) shows a pronounced shift from that found in wild-type cells. The rate of certain types of base substitutions (particularly transitions) is dramatically increased, while gene rearrangements constitute a lower proportion of mutations. These data suggest a lower fidelity of the replication process in the mutator strain.
authors: Imperial Cancer Research Fund, South Mimms, Hertfordshire, United Kingdom.
authors: M A
authors: MA
authors: Caligo
authors: W
authors: W
authors: Armstrong
authors: B J
authors: BJ
authors: Rossiter
authors: M
authors: M
authors: Meuth
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002872:Chromosome Deletion; D006224:Cricetinae; D004351:Drug Resistance / Q000235:genetics; D005784:Gene Amplification; D008727:Methotrexate / Q000494:pharmacology; D008969:Molecular Sequence Data; D009154:Mutation; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*
chemical_list: D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate