Effect of denervation on the expression of glycogen phosphorylase in mouse skeletal muscle.
After sciatectomy of the left hind-limb of C57BL/J mice, a denervation-induced muscular atrophy ensued and was accompanied by a decrease in the specific activity of glycogen phosphorylase to approx. 25% of control values. The cofactor of phosphorylase, pyridoxal 5'-phosphate, was used as a specific label in the determination of the degradation rate of the enzyme following nerve section. After a delay of 3-4 days, phosphorylase was degraded approx, twice as rapidly in the denervated gastrocnemius (0.20 day-1) as in the control muscle (0.12 day-1). The effect of denervation on phosphorylase mRNA was measured by quantitative Northern-blot analysis using a rat skeletal-muscle phosphorylase cDNA probe. After an initial rapid decline, phosphorylase mRNA levels stabilized in denervated muscle at 50% of the value measured in the contralateral control muscle.
Leyland
D M
DM
Department of Biochemistry, University of Liverpool, U.K.
Turner
P C
PC
Beynon
R J
RJ
D000818:Animals; D015152:Blotting, Northern; D005260:Female; D007700:Kinetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009121:Muscle Denervation; D009132:Muscles / Q000201:enzymology* / Q000293:injuries; D006005:Phosphorylases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D012519:Sarcoplasmic Reticulum / Q000201:enzymology; D012584:Sciatic Nerve / Q000502:physiology*
D012333:RNA, Messenger; D006005:Phosphorylases