Bifunctional lipid-transfer: fatty acid-binding proteins in plants.
A cytosolic protein, able to facilitate intermembrane movements of phospholipids in vitro, has been purified to homogeneity from sunflower seedlings. This protein, which has the properties of a lipid-transfer protein (LTP), is also able to bind oleoyl-CoA, as shown by FPLC chromatography. This finding, in addition to previous observations suggesting that a lipid-transfer protein from spinach leaves can bind oleic acid and that oat seedlings contain a fatty acid-binding protein with similar features than lipid transfer proteins, provides a clear demonstration that plant cells contain bifunctional fatty acid/lipid transfer proteins. These proteins can play an active role in fatty acid metabolism which involves movements of oleyl-CoA between intracellular membranes.
Arondel
V
V
Laboratoire de Physiologie Cellulaire (U.R.A. 1180 C.N.R.S.), Université Pierre et Marie Curie, Paris, France.
Vergnolle
C
C
Tchang
F
F
Kader
J C
JC
D000214:Acyl Coenzyme A / Q000378:metabolism; D000595:Amino Acid Sequence; D052179:Antigens, Plant; D002352:Carrier Proteins / Q000737:chemistry / Q000302:isolation & purification*; D002850:Chromatography, Gel; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids / Q000378:metabolism*; D006368:Helianthus / Q000032:analysis*; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D010743:Phospholipids / Q000378:metabolism; D010940:Plant Proteins; D012639:Seeds / Q000032:analysis*
D000214:Acyl Coenzyme A; D052179:Antigens, Plant; D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D009363:Neoplasm Proteins; D010743:Phospholipids; D010940:Plant Proteins; C511920:lipid transfer proteins, plant; C017585:oleoyl-coenzyme A