Effect of hypertension on fibronectin expression in the rat aorta.
Interactions between extracellular fibronectin and vascular cells are thought to influence the phenotype of those cells. To determine if changes in fibronectin expression accompany the phenotypic changes of vascular tissue characteristic of experimental hypertension, steady state mRNA levels for fibronectin were determined in aortae of normotensive and hypertensive rats. A 3-6-fold increase in fibronectin mRNA was observed in aortic tissue of hypertensive rats following 3 weeks of treatment with deoxycorticosterone and salt, whereas if rats were treated only with deoxycorticosterone or salt alone, no changes occurred. The changes were reversed by normalization of blood pressure. The increases observed were localized to aorta and not to the periaortic tissue. Angiotensin II infusion using osmotic minipumps also caused an increase in fibronectin expression. Age-dependent increases in aortic fibronectin mRNA occurred in several rat strains, and the combined effects of hypertension and aging were greater than either variable alone. A clear distinction between the expression of fibronectin mRNA and that for collagen or tropoelastin were found in hypertensive and aging models. Aortic fibronectin was also increased in the hypertensive rats as determined by Western blot analysis. The findings indicate that elevation in blood pressure increases fibronectin expression in rat aorta and suggest that such changes may influence the aortic cellular responses to hypertension.
Takasaki
I
I
Department of Medicine, Boston University School of Medicine, Massachusetts 02118.
Chobanian
A V
AV
Sarzani
R
R
Brecher
P
P
D002001:Adipose Tissue, Brown / Q000503:physiopathology; D000367:Age Factors; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D001011:Aorta / Q000503:physiopathology*; D015152:Blotting, Northern; D015153:Blotting, Western; D003094:Collagen / Q000235:genetics; D003900:Desoxycorticosterone; D004195:Disease Models, Animal; D005353:Fibronectins / Q000235:genetics*; D015870:Gene Expression; D006973:Hypertension / Q000235:genetics*; D009392:Nephrectomy; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011918:Rats, Inbred SHR / Q000378:metabolism; D011919:Rats, Inbred Strains; D014333:Tropoelastin / Q000235:genetics
D005353:Fibronectins; D012333:RNA, Messenger; D014333:Tropoelastin; D000804:Angiotensin II; D003900:Desoxycorticosterone; D003094:Collagen