title: Effect of ADP/ATP antiporter conformational state on the suppression of the nonspecific permeability of the inner mitochondrial membrane by cyclosporine A.
abstract: The influence of the conformational state of ADP/ATP antiporter on the efficiency of the inhibitory effect of cyclosporine A on the Ca2(+)-induced nonspecific permeability of the inner mitochondrial membrane has been studied. Carboxyatractiloside, the inhibitor of ADP/ATP-antiporter, was shown to prevent the cyclosporine A-induced suppression of the nonspecific permeability. The carboxyatractiloside effect was displayed only in mitochondria depleted of adenine nucleotides. Bifunctional SH reagent, phenylarsine oxide, was also able to reverse the effect of cyclosporine A. The data are consistent with the suggestion that cyclosporine A causes suppression of the nonspecific permeability due to its effect on the ADP/ATP antiporter conformation.
authors: A.N. Belozersky Laboratory, M.V. Lomonosov Moscow State University, Department of Biophysics, USSR.
authors: S A
authors: SA
authors: Novgorodov
authors: T I
authors: TI
authors: Gudz
authors: Y E
authors: YE
authors: Kushnareva
authors: D B
authors: DB
authors: Zorov
authors: Y B
authors: YB
authors: Kudrjashov
mesh_terms: D000818:Animals; D001152:Arsenicals / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D003524:Cyclosporins / Q000494:pharmacology*; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000187:drug effects; D008564:Membrane Potentials; D008930:Mitochondria, Liver / Q000187:drug effects*; D000226:Mitochondrial ADP, ATP Translocases / Q000378:metabolism*; D010539:Permeability / Q000187:drug effects; D011487:Protein Conformation; D051381:Rats; D013439:Sulfhydryl Reagents / Q000494:pharmacology
chemical_list: D001152:Arsenicals; D003524:Cyclosporins; D013439:Sulfhydryl Reagents; C029341:oxophenylarsine; D000226:Mitochondrial ADP, ATP Translocases; D002118:Calcium