title: The tricarboxylate carrier from rat liver mitochondria. Purification, reconstitution and kinetic characterization.
abstract: The tricarboxylate carrier from rat liver mitochondria has been purified and reconstituted into phospholipid vesicles. Its activity has been characterized by both a radioactive citrate uptake assay and a coupled enzymatic assay. A Km of 40 microM and a Vmax of 1.56 mumol x min-1 x mg-1 have been determined for the carrier. Cholesterol levels of between 5-10% of total lipid content are shown to cause a decrease in carrier activity.
authors: Institut für Biochemie and Molekularbiologie, Universität Bern, Switzerland.
authors: D M
authors: DM
authors: Glerum
authors: D
authors: D
authors: Claeys
authors: W
authors: W
authors: Mertens
authors: A
authors: A
authors: Azzi
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D002784:Cholesterol / Q000494:pharmacology; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008930:Mitochondria, Liver / Q000378:metabolism*; D051381:Rats
chemical_list: D002352:Carrier Proteins; C020723:citrate-binding transport protein; D002784:Cholesterol