Effects of ethanol extraction and heat treatment of soybean flakes on function and morphology of pig intestine.
Digesta and tissue samples were collected from the intestinal tracts of 27 pigs to determine the relationship between intestinal morphology and the utilization of nutrients from soybean flakes. Soybean flake treatments were under-, intermediate- and over-processed (i.e., 5, 20 and 60 min of autoclaving) either without extraction or with heating before or after extraction with a 55% ethanol-water mixture. Final BW was greatest (P less than .001) for pigs fed soybean flakes given 20 min of heat treatment. There was a trend (P less than .09) for plasma lysine concentrations to be reduced when the unextracted soybean flakes were over-processed (60 min of heat). Differences in the flow rate of DM and N through the ileum and colon reflected differences in DM and N intake, rather than differences in intestinal function. The soybean flake treatments had no effect (P greater than .08) on pH of the contents of the stomach, duodenum, ileum or colon. The ethanol extraction process increased (P less than .001) N digestibility of the soybean flakes, especially when the soybean flakes were underprocessed (interaction, P less than .02). Villus size (area, height and perimeter length) tended to be greater in pigs fed the soybean flakes heated after extraction and(or) exposed to the intermediate level of heat treatment. Indicators of villus shape (villus area/villus height) and proliferative activity (crypt depth and villus height/crypt depth) were not affected by soybean flake treatment (P greater than .08). Ethanol extraction and heat treatment affected the utilization of nutrients from soybean flakes.(ABSTRACT TRUNCATED AT 250 WORDS)
Hancock
J D
JD
University of Nebraska, Lincoln 68583-0908.
Peo
E R
ER
Lewis
A J
AJ
Moxley
R A
RA
D000818:Animals; D004063:Digestion; D000431:Ethanol; D005260:Female; D005511:Food Handling / Q000379:methods*; D005769:Gastrointestinal Motility; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007422:Intestines / Q000033:anatomy & histology / Q000502:physiology*; D008297:Male; D008871:Microvilli / Q000648:ultrastructure; D013025:Glycine max; D013552:Swine / Q000033:anatomy & histology / Q000254:growth & development / Q000502:physiology*; D015430:Weight Gain
D000431:Ethanol