R-plasmid mediated transfer of beta-lactam resistance in Bacteroides fragilis.
We described plasmid mediated transfer of resistance to beta-lactam antibiotics between Bacteroides fragilis strains. Ampicillin-resistance was transferred from B. fragilis strain GAI-10150 to a B. fragilis strain JC-101 with a frequency of 10(-6)/input donor by a filter mating technique. A common plasmid band, named pBFKW1, was found in both the donor and the transconjugants. The plasmid was purified by an ethidium bromide-CsCl ultracentrifugation. The molecular size of the plasmid pBFKW1 which seemed to encode the beta-lactam resistance and beta-lactamase production was estimated ca. 40 kb by the analysis of endonuclease digest. Substrate profile of the enzymes derived from the donor and a transconjugant were of cephalosporinase character.
Yamaoka
K
K
Institute of Anaerobic Bacteriology, School of Medicine, Gifu University, Japan.
Watanabe
K
K
Muto
Y
Y
Katoh
N
N
Ueno
K
K
Tally
F P
FP
D000668:Ampicillin Resistance / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001441:Bacteroides fragilis / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000032:analysis; D004352:Drug Resistance, Microbial / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D010403:Penicillin Resistance / Q000235:genetics; D011815:R Factors; D013379:Substrate Specificity; D001618:beta-Lactamases / Q000032:analysis / Q000235:genetics; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D047090:beta-Lactams; D001618:beta-Lactamases