Progression of neurological disease in thiamin-deficient rats is enhanced by ethanol.
The clinical and neuropathological consequences of either ethanol consumption or thiamin deficiency or both were examined in Wistar rats aged nine weeks divided into five groups and fed one of the following diets: a thiamin-replete (control) diet (A): a thiamin-fortified diet with water (B) or 15% ethanol (C); or a thiamin-deficient diet with water (D) or 15% ethanol (E). Rats fed diets A, B or C for 35 weeks showed no clinical signs of neurological disease at any stage and no significant brain pathology when harvested. Rats fed diets D and E progressed through a common sequence of clinical signs of neurological disease typical of acute thiamin deficiency, viz loss of coat condition, ataxia, opisthotonus and ultimately death within 10-23 weeks. The onset and progression of these stages of neurological disease were significantly earlier and faster (p less than 0.001 for proportion of opisthotonic and ataxic animals at weeks 10 and 15) in the thiamin-deficient rats that received ethanol than in those that did not. At death, the brain pathology in these two groups was limited and similar.
Zimitat
C
C
Department of Biochemistry, University of Queensland, St. Lucia, Australia.
Kril
J
J
Harper
C G
CG
Nixon
P F
PF
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001921:Brain / Q000187:drug effects / Q000473:pathology; D004326:Drinking / Q000187:drug effects; D004435:Eating / Q000187:drug effects; D000431:Ethanol / Q000097:blood / Q000633:toxicity*; D005260:Female; D009929:Organ Size / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013831:Thiamine / Q000378:metabolism; D013832:Thiamine Deficiency / Q000150:complications*; D014899:Wernicke Encephalopathy / Q000209:etiology*
D000431:Ethanol; D013831:Thiamine