[Immune response to a synthetic polysaccharide and protein conjugate].
Synthetic polysaccharide (S-PS) containing aglycone-spacer with a free amino group was really alpha 1,6-mannan with Cn approximately 10. S-PS was transformed into isothiocyanate derivative by treating it with thiophosgene and engaged into reaction with amino group of bovine serum albumin (BSA) lysine residues. Rabbits were immunized with S-PS-BSA conjugate and antibodies to S-PS titres were estimated by means of ELISA. S-PS-BSA conjugate was proved to provoke specific anti-polysaccharide antibodies formation in rabbits.
Makarenko
T A
TA
Kocharova
N A
NA
Tsvetkov
Iu E
IuE
Edvabnaia
L S
LS
Knirel'
Iu A
IuA
Bakanovskiĭ
L V
LV
Kholodkova
E V
EV
Stanislavskiĭ
E S
ES
Kochetkov
N K
NK
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D011134:Polysaccharides / Q000276:immunology*; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology*
D000906:Antibodies; D000941:Antigens; D011134:Polysaccharides; D012710:Serum Albumin, Bovine
Immunnyĭ otvet k kon"iugatu sinteticheskogo polisakharida s belkom.