Synthesis of a deuterated analogue and development of antibody/GC/MS for the determination of nocloprost in plasma.
An analytical method for the determination of the PGE derivative nocloprost in plasma was developed combining the features of both radioimmunoassay and GC/MS. The antibody usually employed in nocloprost radioimmunoassay was coupled to Sepharose 4B and used as a stationary phase for the extraction of the drug. After appropriate derivatisation, nocloprost was determined by GC/MS in the negative ion-chemical ionisation mode. As an internal standard deuterated nocloprost was synthesized and added to the plasma samples before extraction. The extraction recovery was 94% and the limit of detection was 5 pg/ml. Intra- and interassay precision at 100 pg/ml was calculated as 3.3 and 4.0%, respectively.
Krause
W
W
Research Laboratories, Schering AG, Berlin, West Germany.
Jakobs
U
U
Buckenauer
R
R
Biere
H
H
D002138:Calibration; D003903:Deuterium; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007553:Isotope Labeling; D015394:Molecular Structure; D011461:Prostaglandins F, Synthetic / Q000097:blood* / Q000737:chemistry; D011863:Radioimmunoassay / Q000379:methods
D011461:Prostaglandins F, Synthetic; C048895:nocloprost; D003903:Deuterium