R-banding and nonisotopic in situ hybridization: precise localization of the human type II collagen gene (COL2A1).
A new mapping system, based on nonisotopic in situ hybridization combined with fluorescent staining of replicated prometaphase R-bands, is described. Replication of the bands is achieved by treatment of thymidine-synchronized cells with bromodeoxyuridine. The human COL2A1 gene was mapped to band 12q13.11-q13.12 in this manner, to illustrate the potential of the technique for improving the precision of chromosomal mapping and physical ordering of genes.
Takahashi
E
E
Division of Genetics, National Institute of Radiological Sciences, Chiba, Japan.
Hori
T
T
O'Connell
P
P
Leppert
M
M
White
R
R
D002871:Chromosome Banding / Q000379:methods; D002874:Chromosome Mapping / Q000379:methods*; D002881:Chromosomes, Human, Pair 12; D003094:Collagen / Q000235:genetics*; D015342:DNA Probes; D006801:Humans; D007621:Karyotyping; D009693:Nucleic Acid Hybridization
D015342:DNA Probes; D003094:Collagen