title: Sequence analysis and HLA-DR genotyping of a novel HLA-DRw14 allele.
abstract: Analysis of a Japanese population by oligonucleotide genotyping revealed that one Japanese HLA-DRw14 allele had a DRB1 genotype different from that of the known HLA-DRw14-related alleles, DRB1*1401 (DRw14-Dw9) and DRB1*1402 (DRw14-Dw16). The second exon of the DRB1 gene of the novel DRw14 allele (designated DRB1-14c) was amplified enzymatically and sequenced after cloning into a plasmid vector. The amino acid sequence of the first domain in the DR beta 1 chain encoded in the DRB1-14c allele was more similar to that of the DRB1*1401 allele (three amino acid substitutions) than to that of the DRB1*1402 allele (six amino acid substitutions). No polymorphic amino acid residue that could explain the common serologic HLA-DRw14 specificity was identified among the sequences of the three DRw14-related alleles. Sequence-specific oligonucleotides (SSOs) were synthesized on the basis of the DRB1-14c nucleotide sequence and used for genotyping of the Japanese population. These SSOs served as useful probes for identifying the DRB1-14c allele in a wide range of donors.
authors: Department of Transplantation Immunology, Kitasato University School of Medicine, Sagamihara, Japan.
authors: F
authors: F
authors: Obata
authors: I
authors: I
authors: Ito
authors: K
authors: K
authors: Ito
authors: A
authors: A
authors: Abe
authors: M
authors: M
authors: Ohkubo
authors: K
authors: K
authors: Onda
authors: Y G
authors: YG
authors: Yang
authors: K
authors: K
authors: Watanabe
authors: N
authors: N
authors: Kashiwagi
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001402:B-Lymphocytes / Q000276:immunology; D001483:Base Sequence; D015342:DNA Probes; D004591:Electrophoresis, Polyacrylamide Gel; D005838:Genotype; D006684:HLA-DR Antigens / Q000235:genetics*; D059810:HLA-DR Serological Subtypes; D006801:Humans; D007564:Japan; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
chemical_list: D015342:DNA Probes; D006684:HLA-DR Antigens; D059810:HLA-DR Serological Subtypes; C052238:HLA-DR14