Cryopreserved/thawed semen for in vitro fertilization: results from fertile donors and infertile patients.
We evaluated the in vitro fertilization (IVF) outcome in 54 cycles using cryopreserved/thawed semen from fertile donors. Controls were other IVF patients matched by time frame, female age, stimulation protocol, number of pre-embryos transferred, and absence of a male factor using freshly ejaculated normal semen samples. In the study group and controls, respectively, post-thaw swim-up motility was 83.1% and 89.5%; fertilization rate of preovulatory oocytes (91.8%, 95.7%) and ongoing pregnancy rate (PR) per transfer (21.1%, 25.0%) were similar. The excellent fertilization rate with frozen/thawed semen was achieved through high-concentration insemination (0.5 x 10(6) motile sperm/mL). With use of frozen/thawed samples from infertile men (normal and subfertile samples), PR was similar but fertilization rate was lower. Cryopreserved semen is a valuable option for infertile couples in IVF therapy.
Morshedi
M
M
Howard and Georgeanna Jones Institute for Reproductive Medicine, Department of Obstetrics and Gynecology, Eastern Virginia Medical School, Norfolk.
Oehninger
S
S
Veeck
L L
LL
Ertunc
H
H
Bocca
S
S
Acosta
A A
AA
D015925:Cryopreservation; D005260:Female; D005298:Fertility; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D007247:Infertility, Female / Q000503:physiopathology / Q000628:therapy*; D008297:Male; D011247:Pregnancy; D012661:Semen; D013076:Sperm Count; D013081:Sperm Motility; D014019:Tissue Donors