Species-specific lens activation of the thymidine kinase promoter by a single copy of the mouse alpha A-CRYBP1 site and loss of tissue specificity by multimerization.
One copy of the mouse alpha A-crystallin gene alpha A-CRYBP1 site activated the thymidine kinase (tk) promoter in a mouse lens epithelial cell line but not in primary chicken lens cells; multiple copies further activated the tk promoter and extended expression to fibroblasts, B cells, and chicken lens cultures. The loss of lens specificity by multimerization may place selective constraints on the number of alpha A-CRYBP1 sites in the alpha A-crystallin promoter.
Sax
C M
CM
Laboratory of Molecular and Developmental Biology, National Eye Institute, Bethesda, Maryland 20892.
Klement
J F
JF
Piatigorsky
J
J
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D002478:Cells, Cultured; D002642:Chick Embryo; D003459:Crystallins / Q000235:genetics*; D004848:Epithelium / Q000201:enzymology; D015971:Gene Expression Regulation, Enzymologic; D007908:Lens, Crystalline / Q000201:enzymology*; D051379:Mice; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D011401:Promoter Regions, Genetic; D013937:Thymidine Kinase / Q000235:genetics*; D014158:Transcription, Genetic; D014162:Transfection
D003459:Crystallins; D015345:Oligonucleotide Probes; D013937:Thymidine Kinase