[Effects of recombinant erythropoietin on terminal cytoplasmic maturation of megakaryocytes and thrombopoiesis].
The liquid culture system of platelet production was used to monitor the effect of recombinant human erythropoietin (r-EPO) on megakaryocyte maturation and thrombopoiesis. r-EPO, when added to the culture at concentration from 1U/ml to 6U/ml, significantly increased the platelet count in a dose dependent manner, while the megakaryocyte number remained unchanged. The rate of cytoplasmic maturation and DNA synthesis of megakaryocyte were increased by r-EPO. Our data indicate that r-EPO is a potent stimulant for terminal cytoplasmic maturation of megakaryocyte and thrombopoiesis.
Tu
Q
Q
Institute of Radiation Medicine, Beijing.
Zhang
Q X
QX
D000818:Animals; D001792:Blood Platelets / Q000166:cytology*; D002452:Cell Count; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004921:Erythropoietin / Q000494:pharmacology*; D008297:Male; D008533:Megakaryocytes / Q000166:cytology*; D051379:Mice; D010976:Platelet Count; D011994:Recombinant Proteins / Q000494:pharmacology
D011994:Recombinant Proteins; D004921:Erythropoietin; D004247:DNA