title: Sialyltransferase activity in AF10 myeloma cell line.
abstract: A STA was observed in a human-derived myeloma cell line, AF10, that produces IgE paraprotein. The STA in the culture medium of the AF10 myeloma cell line was associated in the 24 to 48 hr period of incubation with IgE biosynthesis, and increased thereafter up to 72 hr, while IgE remained stable. These data support our previous observations that human myeloma cells are associated with a relatively high production of sialyltransferase, which is released to the medium, presumably by a mechanism of shedding.
authors: Hematology Clinic, Golda Medical Center, Petah-Tikva, Sackler School of Medicine, Tel-Aviv University, Israel.
authors: A M
authors: AM
authors: Cohen
authors: D
authors: D
authors: Allalouf
authors: T
authors: T
authors: Wacks
authors: N
authors: N
authors: Lehrer
authors: H
authors: H
authors: Levinsky
mesh_terms: D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis; D009101:Multiple Myeloma / Q000201:enzymology* / Q000276:immunology; D012799:Sialyltransferases / Q000032:analysis*; D014407:Tumor Cells, Cultured; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
chemical_list: D007073:Immunoglobulin E; D012799:Sialyltransferases; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase