Ontogeny of adenosine response in guinea pig heart and aorta.
Effects of maturation on the responses of isolated perfused hearts and aortic rings to adenosine were examined. Dose-response relationships for adenosine were obtained in aortic rings and hearts isolated from immature (5 days) and mature (1-2 mo) guinea pigs. Immature and mature hearts were perfused at constant flows of 9.9 +/- 0.3 and 9.5 +/- 0.4 ml.min-1.g-1, respectively, and displayed basal resistances of 4.8 +/- 0.1 and 6.7 +/- 0.2 mmHg.ml-1.min.g. Immature hearts were more sensitive to exogenous adenosine, displaying a significantly lower 50% effective concentration (EC50, 2.5 x 10(-8) M) than mature hearts (1.1 x 10(-7) M, P less than 0.05). Adenosine induced dilation at a lower threshold dose in immature hearts (3 x 10(-9) M, 6.0 +/- 0.3% relaxation) than in mature hearts (10(-8) M, 3.1 +/- 1.3% relaxation). The time required to elicit 50% of the observed dilation was similar in immature and mature hearts, yet the time required for basal tone to recover by 50% was approximately 100% greater in immature hearts (P less than 0.05). Immature aortic rings, stretched to their optimal resting tensions and contracted with EC85 doses of prostaglandin F2 alpha, displayed a significantly lower EC50 (7.7 x 10(-5) M) than mature rings (1.1 x 10(-4) M, P less than 0.05). The maximum percent response to adenosine was greater in immature vessels (64 +/- 1 vs. 54 +/- 0.4%, P less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
Matherne
G P
GP
Department of Physiology, University of Virginia, Charlottesville 22908.
Headrick
J P
JP
Berne
R M
RM
D000241:Adenosine / Q000494:pharmacology*; D000375:Aging / Q000502:physiology; D000818:Animals; D001011:Aorta / Q000187:drug effects* / Q000254:growth & development; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D006321:Heart / Q000187:drug effects* / Q000254:growth & development; D066298:In Vitro Techniques; D009994:Osmolar Concentration; D010477:Perfusion
D000241:Adenosine