title: Effects of fluoride on rat cancellous bone.
abstract: Female Wistar rats were divided into four groups each fed ad libitum regular rodent chow and fluoridated water at four levels (0, 2, 4, 6 mmol/l) for 3 months. There were significant differences in ash weights between control and fluoridated vertebrae (P less than 0.01). Intact bone fluoride (F) values observed were: 0.04 +/- 0.01% fat-free dry weight (ffdw) for control vertebrae and 0.22-0.34 +/- 0.03% ffdw for fluoridated vertebrae. Deproteinized fluoridated vertebral bone had significantly lower specific surface area (SSA) (from 100 +/- 19 m2/g, P less than 0.01, down to 80 +/- 20 m2/g, P less than 0.001) than the control group (120 +/- 10 m2/g); a finding in support of our hypothesis that more of the bone mineral units in fluoridated bone are older (hence denser) than in normal bone. Static morphometric analysis of toluidine blue-stained sections of the femoral metaphyses showed that both cancellous bone volume/tissue volume (Cn-BV/TV) and trabecular thickness (Tb.Th) increased linearly with NaF dosage (R = 0.99 and 0.97, respectively); with Cn-BV/TV increasing from 21 +/- 7 to 33 +/- 6% (P less than 0.01) and Tb.Th from 76 +/- 8 to 98 +/- 9 microns (P less than 0.01). Similar Cn-BV/TV results were also obtained from von Kossa-stained sections.(ABSTRACT TRUNCATED AT 250 WORDS)
authors: Department of Pathology, Mount Sinai Hospital, University of Toronto, Canada.
authors: P T
authors: PT
authors: Cheng
authors: S M
authors: SM
authors: Bader
mesh_terms: D000818:Animals; D015519:Bone Density / Q000187:drug effects*; D001862:Bone Resorption; D001842:Bone and Bones / Q000033:anatomy & histology / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D010012:Osteogenesis / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D012969:Sodium Fluoride / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D012969:Sodium Fluoride