[Clinical significance of determination of serum RNase activities in patients with eosinophilia--II. Measurements using polyuridylic acid and polycytidylic acid as substrates].
The activities of RNase (RNase-U and RNase-C) were determined in the serum and leukocytes of 277 patients with 14 cases of various kinds of eosinophilia (not less than 10(3)/microliters), 28 cases of chronic myelocytic leukemia (CML), using polyuridylic acid and polycytidylic acid as synthetic substrates according to the method of Raddi et al. Serum RNase-U activity, serum RNase-C activity and the activity ratio (U/C x 10(-3)) were 55 +/- 14 U, 1,280 +/- 235 U and 44 +/- 11 (mean +/- SD), 196 +/- 137, 1,992 +/- 1,134 U and 97 +/- 38, and 110 +/- 50 U, 1,854 +/- 625 U and 65 +/- 13 for normal subjects, eosinophilia and CML (untreated), respectively. U/C ratio in eosinophilia and CML (untreated) showed a highly significant positive correlation (p less than 0.001) with peripheral eosinophil count; the activity of serum RNase-U per cells in the supernatant of eosinophil homogenate rose significantly (p less than 0.001) compared with that of lymphocytes or granulocytes. Besides, serum and eosinophil RNase-U had a similar optimal pH. These results suggested that serum RNase-U in eosinophilia originated mostly from eosinophils and its rise was correlated strongly with the increase in eosinophils.
Maruoka
K
K
1st Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health, Kitakyushu.
Yamanaka
M
M
Misago
M
M
Nakata
K
K
Tsukada
J
J
Nagata
K
K
Sato
T
T
Mori
N
N
Oda
S
S
Chiba
S
S
D004796:Clinical Enzyme Tests; D004802:Eosinophilia / Q000175:diagnosis* / Q000209:etiology; D006402:Hematologic Diseases / Q000150:complications; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007674:Kidney Diseases / Q000150:complications; D011066:Poly C; D011072:Poly U; D012260:Ribonucleases / Q000097:blood*
D011072:Poly U; D011066:Poly C; D012260:Ribonucleases