Analysis of cis-acting promoter elements using microinjected synthetic oligonucleotides.
The transferrin receptor (TR) is expressed in a proliferation-dependent manner that requires transcriptional activation of its gene. Furthermore TR function is required for the DNA synthetic phase of the cell cycle. Oligonucleotides encoding promoter elements within the human transferrin receptor gene were injected into quiescent fibroblasts. The cells were then serum stimulated and the effect of the oligonucleotides on nuclear labeling with [3H]thymidine was determined. Inhibition was observed for oligonucleotides corresponding to a specific region of the promoter.
Miskimins
W K
WK
Department of Biological Sciences, University of South Carolina, Columbia 29208.
Brown
D B
DB
D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle / Q000502:physiology; D002460:Cell Line; D004247:DNA / Q000235:genetics / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D015870:Gene Expression / Q000235:genetics; D051379:Mice; D008845:Microinjections; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011990:Receptors, Transferrin / Q000235:genetics / Q000378:metabolism; D016196:S Phase / Q000502:physiology; D013936:Thymidine / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics; D014316:Tritium
D009841:Oligonucleotides; D011990:Receptors, Transferrin; D014316:Tritium; D004247:DNA; D013936:Thymidine