From biopsy to automatic diagnosis.
High resolution two-dimensional gel electrophoresis is a very powerful biochemical tool for analysis of complex protein mixtures. In well defined situations, protein maps, obtained from tissue biopsies or biological fluids by this technique, can be automatically analyzed by computer. Some polypeptide patterns are the fingerprints of diseases. Applying clustering algorithm and learning techniques, the prototype expert system MELANIE recognized patterns and associated the correct diagnosis to the specific pattern.
Hochstrasser
D F
DF
Medicine Department, Geneva University Hospital.
Funk
M
M
Appel
R D
RD
Pun
T
T
James
R W
RW
Hochstrasser
A C
AC
Scherrer
J R
JR
Pellegrini
C
C
Müller
A F
AF
D003936:Diagnosis, Computer-Assisted; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005103:Expert Systems; D006801:Humans; D007525:Isoelectric Focusing; D010449:Peptide Mapping / Q000379:methods*