Immunohistochemical detection of cytochrome P450 isoenzymes in cultured human epidermal cells.
We used specific monoclonal antibodies (MAb) to human cytochrome P450 isoenzymes to determine the presence of these proteins in human epidermal cells. Two MAb (P450-5 and P450-8) recognize major forms of hepatic cytochrome P450 involved in biotransformation of xenobiotics. A third MAb, to cytochrome P450-9, is not fully characterized. The proteins were determined by the indirect immunoperoxidase technique after fixation with methanol and acetone. Biopsy materials for cultured keratinocytes, i.e., foreskin and hair follicles, contained the two major forms of cytochrome P450. In cultured keratinocytes derived from hair follicles the proteins were undetectable, whereas the keratinocytes derived from foreskin continued to express the two major forms of hepatic cytochrome P450. Cultured human fibroblasts and a human keratinocyte cell line (SVK14) showed staining similar to that of the foreskin keratinocytes. Cytochrome P450-9 was detectable only in human hepatocytes. The results indicate that, under the culture conditions applied, cultured human foreskin cells and the cell line SVK14 continue to express specific cytochrome P450 isoenzymes in culture, in contrast to hair follicle keratinocytes.
Van Pelt
F N
FN
RCC NOTOX BV, Hertogenbosch, The Netherlands.
Meierink
Y J
YJ
Blaauboer
B J
BJ
Weterings
P J
PJ
D000911:Antibodies, Monoclonal; D002460:Cell Line; D002478:Cells, Cultured; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis*; D004817:Epidermis / Q000201:enzymology*; D005404:Fixatives; D006197:Hair / Q000201:enzymology; D006801:Humans; D007124:Immunoenzyme Techniques; D007527:Isoenzymes / Q000032:analysis*; D015603:Keratinocytes / Q000201:enzymology
D000911:Antibodies, Monoclonal; D005404:Fixatives; D007527:Isoenzymes; D003577:Cytochrome P-450 Enzyme System