Crystallization of the Fab from a human monoclonal antibody against gp 41 of human immunodeficiency virus type I.
A monoclonal IgG antibody directed against gp 41 from the human immunodeficiency virus (HIV-1) has been crystallized in both intact and Fab forms. Crystals of the intact antibody grow as tetragonal-like prisms too small for conventional X-ray analysis. However, the Fab portion of the antibody produces suitable plate-like crystals which belong to the space group P2(1)2(1)2(1) with unit cell constants of a = 66.5 A, b = 74.3 A and c = 105.3 A. There is one molecule of Fab in the asymmetric unit. The Fab crystals show diffraction to d-spacings less than 3.0 A.
Casale
E
E
Space Science Laboratory, Marshall Space Flight Center, Huntsville, AL 35812.
Wenisch
E
E
He
X M
XM
Righetti
P G
PG
Snyder
R S
RS
Jungbauer
A
A
Tauer
C
C
Ruker
F
F
Carter
D C
DC
D000911:Antibodies, Monoclonal / Q000737:chemistry; D000937:Antigen-Antibody Reactions; D003460:Crystallization; D015700:HIV Envelope Protein gp41 / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry; D014961:X-Ray Diffraction
D000911:Antibodies, Monoclonal; D015700:HIV Envelope Protein gp41; D007140:Immunoglobulin Fab Fragments