[The antibody activity to synthetic C-terminal fragments of the heavy-chain hemagglutinins of influenza virus A subtypes H1 and H3].
C-terminal peptide fragments of the heavy chain (HA1) of influenza virus, subtypes H1 and H3, hemagglutinins were synthesized. Rabbits and guinea pigs immunized with peptides H3 (314-328) and H1 (314-328) conjugates with BSA developed high immune response to conjugated peptides. Solid-phase RIA demonstrated interaction of antisera to peptide H3 (314-328) with A/Aichi/2/68 virus which was inhibited by peptides H3 (314-328) and H3 (317-328) but not by H3 (320-328), that is, antibodies interacted predominantly with amino acids 317-323 HA1. Anti-H3 (314-328)-BSA showed a marked activity in H1 and NT with a number of H3 subtype viruses. Antisera to peptide H1 (314-328) reacted with A/PR/8/34 virus insignificantly and were also inactive in H1 and NT with subtype H1 viruses.
Mazurkova
N A
NA
Kalashnikov
V V
VV
Mizenko
G A
GA
Samukov
V V
VV
Podcherniaeva
R Ia
RIa
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000937:Antigen-Antibody Reactions / Q000276:immunology; D002642:Chick Embryo; D006168:Guinea Pigs; D006385:Hemagglutination Inhibition Tests; D006389:Hemagglutinins, Viral / Q000276:immunology*; D007114:Immunization; D009980:Influenza A virus / Q000276:immunology*; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000276:immunology*; D011817:Rabbits; D011863:Radioimmunoassay
D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral; D010446:Peptide Fragments
Issledovanie aktivnosti antitel k sinteticheskim C-kontsevym fragmentam tiazheloĭ tsepi gemaggliutininov virusa grippa A podtipov H1 i H3.