[The relationship of damage to and death of ascitic tumor cells during starvation to the ATP and free calcium content in the cells].
Ascite tumor cells EL-4 were incubated in conditions of energy starvation (Hanks salt solution with rothenone and without glucose) at 37 degrees C for 3 hours. Under these conditions, some structural cell damages appeared within the first hours: enlarging and flattening of the cells, blebbing, vacuolization of the cytoplasm, nuclear chromatin condensation. Later on, a share of cells with obvious damage decreased, whereas that of the cells stained with trypan blue (dead cells) much increased (up to 90% after a 3 hour incubation). The cellular ATP decreased abruptly (up to 10% of the control) during the first 10 minutes of starvation. Free Ca2+ concentration increased within 1 hour of incubation more than two-fold. The conditions promoting Ca2+ influx (ionophore A23187 + Ca2+ in medium) accelerated the damage and cell death. However, the increase in free Ca2+ concentration did not trigger any damage in the energy-starved cells, since in the Ca2(+)-depleted medium (no increase in free Ca2(+)-concentration) the development of damages was not prevented. The damage initiation was irreversible: the addition of glucose to cell suspensions after 0.5-1 hour of their incubation in energy-starved condition did not prevent the development of damage, while ATP content in these cells was much increased.
Gabaĭ
V L
VL
Makarova
Iu M
IuM
Guliaev
V A
VA
Mosin
A F
AF
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002470:Cell Survival / Q000502:physiology; D004734:Energy Metabolism / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D013050:Spectrometry, Fluorescence; D013217:Starvation / Q000378:metabolism* / Q000473:pathology; D013945:Thymoma / Q000378:metabolism* / Q000648:ultrastructure; D013953:Thymus Neoplasms / Q000378:metabolism* / Q000648:ultrastructure; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000648:ultrastructure
D000255:Adenosine Triphosphate; D002118:Calcium
Zavisimost' povrezhdeniia i gibeli astsitnykh opukholevykh kletok pri golodanii ot soderzhaniia v kletkakh ATF i svobodnogo kal'tsiia.