title: Comparison of two ascitic fluid culture methods in cirrhotic patients with spontaneous bacterial peritonitis.
abstract: The conventional method of ascitic fluid culture detects bacteria in only 50% of cirrhotic patients with neutrocytic ascites and suspected spontaneous bacterial peritonitis (SBP). We have prospectively compared two ascites culture methods in cirrhotic patients with spontaneous bacterial peritonitis: 1) conventional (on chocolate agar, blood agar, Mac Conkay agar, and thioglycolate broth), and 2) modified [inoculation of 10 ml of ascites in a tryptic soy broth (TSB) blood culture bottle at the patient's bedside]. In a 21-month period, 70 episodes of SBP were diagnosed according to our criteria in 60 cirrhotic patients. Both culture methods were performed simultaneously. The conventional grew bacteria in 40 episodes (57%), whereas the modified grew bacteria in 54 episodes (77%), a significantly higher sensitivity (p = 0.0001). In 16 cases (23%), ascitic culture was negative by both methods. The mortality rate was higher among patients with culture-positive SBP than those with culture-negative SBP (46% vs 37%), but did not reach statistical significance. We conclude that ascitic fluid inoculated into a TSB blood culture bottle at the patient's bedside should be used routinely for ascites culture in cirrhotic patients.
authors: Department of Gastroenterology, Hospital de Bellvitge Princeps d'Espanya, Barcelona.
authors: J
authors: J
authors: Castellote
authors: X
authors: X
authors: Xiol
authors: R
authors: R
authors: Verdaguer
authors: J
authors: J
authors: Ribes
authors: J
authors: J
authors: Guardiola
authors: A
authors: A
authors: Gimenez
authors: L
authors: L
authors: Casais
mesh_terms: D001202:Ascitic Fluid / Q000382:microbiology*; D001424:Bacterial Infections / Q000150:complications / Q000382:microbiology*; D001431:Bacteriological Techniques; D003470:Culture Media; D006801:Humans; D008103:Liver Cirrhosis / Q000150:complications*; D010538:Peritonitis / Q000150:complications / Q000382:microbiology*; D011446:Prospective Studies
chemical_list: D003470:Culture Media