title: Liver fatty acid-binding protein in two cases of human lipid storage.
abstract: FABPs in the various tissues play an important role in the intracellular fatty acid transport and metabolism. Reye's syndrome (RS) and multisystemic lipid storage (MLS) are human disorders characterized by a disturbance of lipid metabolism of unknown etiology. We investigated for the first time L-FABP in these two conditions. Affinity purified antibodies against chicken L-FABP were raised in rabbits, and found to cross-react specifically with partially purified human L-FABP. L-FABP content in liver samples of two patients with RS and MLS was investigated by immuno-histochemistry, SDS-PAGE and ELISA. L-FABP immuno-histochemistry showed increased reactivity in the liver of RS patient and normal reactivity in MLS liver. L-FABP increase in RS liver was confirmed by densitometry of SDS-PAGE and ELISA method. By these two methods the increase amounted to 180% and 199% (p less than 0.02), respectively, as compared to controls. A possible role of L-FABP in the pathogenesis of RS is discussed.
authors: Department of Neurology, University of Padova, Italy.
authors: L
authors: L
authors: Vergani
authors: M
authors: M
authors: Fanin
authors: A
authors: A
authors: Martinuzzi
authors: A
authors: A
authors: Galassi
authors: A
authors: A
authors: Appi
authors: R
authors: R
authors: Carrozzo
authors: M
authors: M
authors: Rosa
authors: C
authors: C
authors: Angelini
mesh_terms: D000293:Adolescent; D000818:Animals; D000906:Antibodies / Q000276:immunology; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism*; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005260:Female; D006801:Humans; D008052:Lipid Metabolism, Inborn Errors / Q000378:metabolism* / Q000473:pathology; D008099:Liver / Q000648:ultrastructure; D008107:Liver Diseases / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins; D012202:Reye Syndrome / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D025521:Tumor Suppressor Proteins
chemical_list: D000906:Antibodies; D002352:Carrier Proteins; C493319:FABP1 protein, human; C107608:FABP7 protein, human; C493320:Fabp1 protein, mouse; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D009363:Neoplasm Proteins; D025521:Tumor Suppressor Proteins