[The formation of a test system for assessing the safety of different types of pertussis preparations: their cytotoxic action on mouse thymocytes in vitro].
The test for the evaluation of the toxicity of different types of pertussis preparations as manifested by their in vitro influence on mouse thymic cells (T test) has been finally worked out. The use of the T test has made it possible to reveal the nonstandard character of the production lots of adsorbed diphtheria-pertussis-tetanus vaccines, both whole-cell vaccine and Japanese acellular vaccine. The degree of the in vitro damaging action of pertussis preparations on mouse thymic cells greatly depends on the residual content of Bordetella pertussis nontoxoidized toxin which, in contrast to B. pertussis lipopolysaccharide and filamentous hemagglutinin, produces pronounced cytotoxic action on mouse thymic cells.
Nuriddinova
N R
NR
Lapaeva
I A
IA
Guseva
M V
MV
Vafakulov
B Kh
BKh
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D015721:Diphtheria-Tetanus-Pertussis Vaccine / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D007928:Lethal Dose 50; D051379:Mice; D008808:Mice, Inbred CBA; D010567:Pertussis Vaccine / Q000633:toxicity*; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects*; D013997:Time Factors
D015721:Diphtheria-Tetanus-Pertussis Vaccine; D010567:Pertussis Vaccine
K formirovaniiu sistemy testov otsenki bezopasnosti kokliushnykh preparatov razlichnogo tipa: tsitotoksicheskoe deĭstvie na timotsity mysheĭ in vitro.