Binding of 125I-labelled thyrotrophin to transfected human thyrocytes (SGHTL-34 cells).
The binding of 125I-labelled bovine TSH to a human thyroid cell line (SGHTL-34) has been studied. Binding to hormonally responsive cells was time dependent, specific and reversible. Scatchard analysis of the binding data indicated the presence of a single binding site with high affinity (intrinsic dissociation constant (Kd) = 0.25 +/- 0.08 nmol/l; mean +/- S.E.M.; n = 4) and low capacity (maximum binding (Bmax) = 104 +/- 29 fmol/mg protein; mean +/- S.E.M.; n = 4). Hill plots confirmed the presence of a single site. Kinetic data demonstrated close agreement between the Kd and Bmax obtained from the competition data (Kd = 0.23 +/- 0.35 nmol/l; Bmax = 161 +/- 83 fmol/mg protein; n = 6).
Taylor
A H
AH
Department of Cellular and Molecular Sciences, St George's Hospital Medical School, London.
Whitley
G S
GS
Nussey
S S
SS
D002460:Cell Line; D006801:Humans; D007457:Iodine Radioisotopes; D007700:Kinetics; D011485:Protein Binding; D011869:Radioligand Assay; D013961:Thyroid Gland / Q000166:cytology / Q000378:metabolism*; D013972:Thyrotropin / Q000032:analysis / Q000378:metabolism*; D013997:Time Factors
D007457:Iodine Radioisotopes; D013972:Thyrotropin