Effect of insulin-like growth factor I on deoxyribonucleic acid synthesis in cultured human granulosa cells.
Insulin-like growth factor I (IGF-I) has been proposed to be an autocrine/paracrine factor involved in granulosa cell proliferation and differentiation. The present study focuses on a possible mitogenic effect of IGF-I in human granulosa cells. Insulin-like growth factor I (1 to 10 ng/mL) significantly stimulated 3H-thymidine incorporation in granulosa cells obtained from both natural cycles and from patients stimulated for in vitro fertilization, whereas luteinizing hormone (LH, 10 ng/mL), follicle-stimulating hormone (FSH, 10 ng/mL) and epidermal growth factor (EGF, 10 ng/mL) had no apparent effect on deoxyribonucleic acid (DNA) synthesis under these conditions. Luteinizing hormone and FSH stimulated progesterone secretion whereas IGF-I and EGF were without effect. Our observations that IGF-I stimulates DNA synthesis in human granulosa cells is in agreement with previous reports, in other species, indicating that IGF-I might be of importance for granulosa cell proliferation.
Olsson
J H
JH
Department of Obstetrics and Gynecology, University of Göteborg, Sweden.
Carlsson
B
B
Hillensjö
T
T
D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism*; D005260:Female; D005307:Fertilization in Vitro; D006062:Gonadotropins / Q000494:pharmacology; D006107:Granulosa Cells / Q000378:metabolism*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D012016:Reference Values; D013936:Thymidine
D006062:Gonadotropins; D007334:Insulin-Like Growth Factor I; D004247:DNA; D013936:Thymidine