Differentiation of a human B cell hybridoma with interleukin-2 receptor.
A 6-thioguanine-neo-resistant clone, Raji 6-TGR-neoR was fused with pokeweed mitogen-stimulated B cells (PWM-B cells) obtained from a normal healthy donor, and selected by a new selection medium (HAT-neo). Two hybrid clones, HYNI-1 and HYNI-2, were established. These clones expressed surface IgG and HLA class II antigens derived from PWM-B cells, and they had about 20 more chromosomes than Raji 6-TGR-neoR. More significantly, one clone, HYNI-2, expressed interleukin-2 receptor on the cell surface, and generated IgG when stimulated with recombinant interleukin-2. These findings indicate that the HYNI-2 is a good model for studying human B cell differentiation.
Ikewaki
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Department of Microbiology, Kitasato University School of Nursing.
D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D001402:B-Lymphocytes / Q000187:drug effects / Q000473:pathology*; D002051:Burkitt Lymphoma / Q000473:pathology; D002454:Cell Differentiation / Q000187:drug effects; D002459:Cell Fusion; D006801:Humans; D006825:Hybridomas / Q000187:drug effects / Q000276:immunology / Q000473:pathology*; D007074:Immunoglobulin G / Q000096:biosynthesis; D007376:Interleukin-2 / Q000494:pharmacology; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000473:pathology
D000911:Antibodies, Monoclonal; D000944:Antigens, Differentiation, B-Lymphocyte; D007074:Immunoglobulin G; D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins