Two different HNF1-like transcription activators in the liver bind to the same region of the rat aldolase B promoter.
Coexistence of two different HNF1-like factors (AlF-A1 and AlF-A2) in rat liver is described. The two factors had similar molecular weight and the same sequence-specificity in DNA-binding. One of these factors AlF-A1 was retained on wheat germ agglutinin-sepharose column and eluted from it with N-acetylglucosamine, while AlF-A2 was not, suggesting that AlF-A1 is glycosylated. Using rat brain nuclear extract, both factors stimulated transcription in vitro, but their stimulatory effects differed from each other.
Ito
K
K
Department of Biochemistry, Yamagata University School of Medicine, Japan.
Tanaka
T
T
Tsutsumi
R
R
Ishikawa
K
K
Tsutsumi
K
K
D000818:Animals; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics*; D051537:Hepatocyte Nuclear Factor 1; D051538:Hepatocyte Nuclear Factor 1-alpha; D051539:Hepatocyte Nuclear Factor 1-beta; D008099:Liver / Q000737:chemistry*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009687:Nuclear Proteins; D011401:Promoter Regions, Genetic; D051381:Rats; D014157:Transcription Factors / Q000302:isolation & purification* / Q000494:pharmacology; D014158:Transcription, Genetic; D015533:Transcriptional Activation
D004268:DNA-Binding Proteins; D051538:Hepatocyte Nuclear Factor 1-alpha; C496489:Hnf1a protein, rat; D009687:Nuclear Proteins; D014157:Transcription Factors; D051537:Hepatocyte Nuclear Factor 1; D051539:Hepatocyte Nuclear Factor 1-beta; D004247:DNA; D005634:Fructose-Bisphosphate Aldolase