Perfluoro-N-decanoic acid effects on enzymes of fatty acid metabolism.
In vitro perfluorodecanoate (PFDA) effects on Pseudomonas acyl-CoA synthetase, Candida acyl-CoA oxidase and pigeon muscle carnitine acetyltransferase were examined. Synthetase made little PFDA-CoA from PFDA. It used palmitate, oleate, laurate and decanoate more extensively. PFDA inhibited acyl-CoA formation from these acids. Palmitoyl-CoA formation was affected most. That of decanoyl-CoA was affected least. Inhibitions appeared to be competitive. Acyl-CoA oxidase test substrates were palmitoyl-CoA, lauroyl-CoA and decanoyl-CoA. Oxidase preferred C-10 and C-12 acyl-CoAs. PFDA inhibited oxidation of C-10 and C-12 acyl-CoAs more than that of palmitoyl-CoA. Inhibitions with C-16 and C-10 acyl-CoAs were competitive, KIs 593 +/- 150 and 76 +/- 6.0 microM. Acetyl-CoA was the best acetyltransferase substrate. C-2 to C-8 transfer from acyl-CoAs was inhibited similarly by PFDA. Inhibitions of C-2 and C-8 transfer were competitive and non-competitive, respectively, KIs 111 +/- 15 and 76 +/- 28 microM.
Singer
S S
SS
Chemistry Department, University of Dayton, OH 43469.
Andersen
M E
ME
George
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ME
D000818:Animals; D002332:Carnitine O-Acetyltransferase / Q000378:metabolism*; D003066:Coenzyme A Ligases / Q000378:metabolism*; D010856:Columbidae; D003652:Decanoic Acids / Q000494:pharmacology*; D005227:Fatty Acids / Q000378:metabolism*; D005466:Fluorocarbons / Q000494:pharmacology*; D007700:Kinetics; D009130:Muscle, Smooth / Q000187:drug effects* / Q000201:enzymology
D003652:Decanoic Acids; D005227:Fatty Acids; D005466:Fluorocarbons; C036567:perfluorodecanoic acid; D002332:Carnitine O-Acetyltransferase; D003066:Coenzyme A Ligases