Catabolism of 3-hydroxybenzoate by the gentisate pathway in Klebsiella pneumoniae M5a1.
Growth of Klebsiella pneumoniae M5a1 on 3-hydroxybenzoate leads to the induction of 3-hydroxybenzoate monooxygenase, 2,5-dihydroxybenzoate dioxygenase, maleylpyruvate isomerase and fumaryl-pyruvate hydrolase. Growth in the presence of 2,5-dihydroxybenzoate also induces all of these enzymes including the 3-hydroxybenzoate monooxygenase which is not required for 2,5-dihydroxybenzoate catabolism. Mutants defective in 3-hydroxybenzoate monooxygenase fail to grow on 3-hydroxybenzoate but grow normally on 2,5-dihydroxybenzoate. Mutants lacking maleylpyruvate isomerase fail to grow on 3-hydroxybenzoate and 2,5-dihydroxybenzoate. Both kinds of mutants grow normally on 3,4-dihydroxybenzoate. Mutants defective in maleylpyruvate isomerase accumulate maleylpyruvate when exposed to 3-hydroxybenzoate and growth is inhibited. Secondary mutants that have additionally lost 3-hydroxybenzoate monooxygenase are no longer inhibited by the presence of 3-hydroxybenzoate. The 3-hydroxybenzoate monooxygenase gene (mhbM) and the maleylpyruvate isomerase gene (mhbI) are 100% co-transducible by P1 phage.
Jones
D C
DC
Department of Biochemistry, University of Leicester, UK.
Cooper
R A
RA
D002852:Chromatography, Ion Exchange; D005841:Gentisates / Q000378:metabolism*; D062385:Hydroxybenzoates / Q000378:metabolism*; D007535:Isomerases / Q000235:genetics / Q000378:metabolism; D007711:Klebsiella pneumoniae / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D010084:Oxidation-Reduction; D014161:Transduction, Genetic; D019745:cis-trans-Isomerases
D005841:Gentisates; D062385:Hydroxybenzoates; C032948:3-hydroxybenzoic acid; C009091:protocatechuic acid; D006899:Mixed Function Oxygenases; C021385:3-hydroxybenzoate-4-monooxygenase; D007535:Isomerases; D019745:cis-trans-Isomerases; C046138:maleylpyruvate isomerase; C010925:2,5-dihydroxybenzoic acid