Countercurrent immunoelectrophoresis in the diagnosis of Taenia hydatigena cysticercosis in goats.
The procedure of countercurrent immunoelectrophoresis in the diagnosis of Taenia hydatigena cysticercosis in goats was carried out for antemortem diagnosis of T. hydatigena cysticercosis in experimentally and naturally infected goats. The antigens of cyst fluid, scolex and membrane of T. hydatigena metacestodes were purified and compared. The sensitivity of the test in experimentally and naturally infected goats was 57.1 and 52.5%, respectively, whereas its specificity using antisera raised against T. solium cysticercosis, hydatid cyst and Fasciola gigantica was 66.7 and 83.4% with partially purified and fractionated antigens, respectively. Of all three antigens, the cyst fluid antigen was found to be most reactive. The test could be employed for antemortem diagnosis of T. hydatigena cysticercosis using purified antigen.
Deka
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DK
Department of Parasitology, College of Veterinary Sciences, G.B. Pant University of Agriculture & Technology, Uttar Pradesh, India.
Gaur
S N
SN
D000818:Animals; D003378:Counterimmunoelectrophoresis; D003429:Cross Reactions; D003551:Cysticercosis / Q000175:diagnosis / Q000662:veterinary*; D003552:Cysticercus / Q000276:immunology / Q000302:isolation & purification*; D015511:Goat Diseases / Q000175:diagnosis*; D006041:Goats; D011237:Predictive Value of Tests