Solasodine glycosides. In vitro preferential cytotoxicity for human cancer cells.
Solamargine [(22R,25R)-spiro-5-en-3 beta-yl-alpha-L-rhamnopyranosyl- (1----2glu)-O-alpha-L-rhamnopyranozyl (1----4glu)-beta-D-glucopyranoze], a glycoside of solasodine preferentially inhibits the uptake of tritiated thymidine by cancer cells. In contrast, solamargine at equivalent concentration, and the mono- and diglycosides of solasodine have a limited effect on the uptake of tritiated thymidine for other cell types, including unstimulated lymphocytes and lymphocytes stimulated with Con A. In contrast the solasodine glycosides do not inhibit the uptake of tritiated thymidine by lymphocytes stimulated with PHA or PWM. The inhibition of tritiated thymidine uptake by solamargine and the mono- and di-glycosides of solasodine are dependent upon their cellular uptake by endogenous endocytic lectins (EELs). The mode of action of the solasodine glycosides, in particular solamargine, appears to be the induction of cell lysis, as determined by morphological examination.
Daunter
B
B
University of Queensland Department of Obstetrics and Gynaecology, Herston, Australia.
Cham
B E
BE
D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006027:Glycosides / Q000037:antagonists & inhibitors / Q000493:pharmacokinetics / Q000494:pharmacology*; D006367:HeLa Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D037102:Lectins / Q000378:metabolism; D007928:Lethal Dose 50; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects; D008969:Molecular Sequence Data; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology; D012991:Solanaceous Alkaloids / Q000037:antagonists & inhibitors / Q000493:pharmacokinetics / Q000494:pharmacology*; D013936:Thymidine / Q000493:pharmacokinetics; D014316:Tritium; D014407:Tumor Cells, Cultured
D002241:Carbohydrates; D006027:Glycosides; D037102:Lectins; D012991:Solanaceous Alkaloids; D014316:Tritium; C012037:solasodine; D013936:Thymidine