title: Reversible inhibition of fertility in mice by passive immunization with anticumulus oophorus antibodies.
abstract: The mucified cumulus oophorus represents an outer enveloping layer around ovulated mammalian oocytes. This coat in its definitive expanded form appears late in the preovulatory development as a result of intensive secretion of intercellular matrix by cumulus cells. We have shown recently that antibodies to the cumulus matrix inhibit human fertilization in vitro. This study was undertaken to assess, in an animal model, the effects of anticumulus oophorus antibodies on fertility by use of different passive immunization protocols. A purified anticumulus immunoglobulin fraction was prepared from hyperimmune rabbit serum and administered at different times before and after mating to mice superovulated with equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG). A dose-dependent negative effect of this anticumulus antibody preparation on the number of fertilized eggs recovered from the oviducts of treated animals was observed when the antibodies were given before mating. High antibody doses also interfered with oocyte maturation and ovulation if applied on the day of eCG treatment, but no effects on these processes were found when the antibodies were given on the day of hCG treatment. The antifertility effect of anticumulus antibodies was reversible and the antibodies did not affect postfertilization development. These findings make cumulus oophorus antigens serious candidates for the development of a contraceptive vaccine.
authors: Unité 187, Institut National de la Santé et de la Recherche Médicale, Hôpital Antoine-Béclère, Clamart, France.
authors: J
authors: J
authors: Tesarik
authors: J
authors: J
authors: Testart
authors: G
authors: G
authors: Leca
authors: F
authors: F
authors: Nomé
mesh_terms: D000818:Animals; D000906:Antibodies; D003269:Contraception, Immunologic; D004305:Dose-Response Relationship, Drug; D005260:Female; D005298:Fertility / Q000276:immunology*; D005306:Fertilization / Q000276:immunology; D007116:Immunization, Passive / Q000379:methods*; D051379:Mice; D009865:Oocytes / Q000276:immunology*; D009866:Oogenesis / Q000276:immunology; D010060:Ovulation / Q000276:immunology; D010865:Pilot Projects
chemical_list: D000906:Antibodies