title: A protocol for the purification of bovine serum albumin free of deoxyribonuclease activity.
abstract: Bovine serum albumin, free of deoxyribonuclease activity, was obtained in our laboratory using ion-exchange chromatography followed by acetylation. Chromatography on four different resins (DEAE-52, P-11, hydroxylapatite and Q Sepharose fast-flow) was examined. Fractions from Q Sepharose chromatography, eluted with a linear gradient 0-1.0 M NaCl and subsequently acetylated, proved to be the most effective method for obtaining deoxyribonuclease-free bovine serum albumin.
authors: Center for Genetic Engineering and Biotechnology, Havana, Cuba.
authors: L E
authors: LE
authors: Trujillo
authors: L
authors: L
authors: Castellanos
authors: O
authors: O
authors: Garcia
authors: L
authors: L
authors: Herrera
mesh_terms: D000107:Acetylation; D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange / Q000379:methods*; D003851:Deoxyribonucleases / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000302:isolation & purification*
chemical_list: D012710:Serum Albumin, Bovine; D003851:Deoxyribonucleases