title: Cloning and nucleotide sequence of the Salmonella typhimurium pepM gene.
abstract: The pepM gene coding for a methionine-specific aminopeptidase was cloned from Salmonella typhimurium and its nucleotide sequence determined. The gene encoded a 264 amino acid protein that was homologous to a similar protein from Escherichia coli. The sequence of an overproducer mutant allele, pepM100, contained a single base change in the likely--35 region of the pepM promoter that increased its homology to the consensus promoter sequence. A region downstream from the pepM coding sequence contained extensive inverted repeats and was homologous to sequences found elsewhere in both Salmonella and other bacterial species.
authors: Biogen S.A., Geneva, Switzerland.
authors: N R
authors: NR
authors: Movva
authors: D
authors: D
authors: Semon
authors: C
authors: C
authors: Meyer
authors: E
authors: E
authors: Kawashima
authors: P
authors: P
authors: Wingfield
authors: J L
authors: JL
authors: Miller
authors: C G
authors: CG
authors: Miller
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D005800:Genes, Fungal; D063208:Methionyl Aminopeptidases; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic; D012486:Salmonella typhimurium / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000626:Aminopeptidases; D063208:Methionyl Aminopeptidases