Characterization of liver-specific expression of rat uricase using monoclonal antibodies and cloned cDNAs.
Tissue distribution of uricase (urate oxidase, EC 1.7.3.3) was studied by immunoblotting and RNA slot blot analysis. For immunoblotting, highly specific monoclonal antibodies against rat liver uricase were obtained, and for mRNA detection, a cloned uricase cDNA was used. Among seven tissues studied, uricase was immunologically detected only in the liver. The contents of uricase in other tissues, i.e., brain, thymus, heart, spleen, kidney and lactating mammary gland, were estimated to be less than 2% of that in the liver. Uricase mRNA was also detected only in the liver. The steady-state level of the mRNA in the isolated hepatocytes was relatively constant during the 8-day culture period when compared with those of other mRNAs expressed in the liver, suggesting a unique control mechanism of its expression.
Motojima
K
K
Department of Biochemistry, School of Pharmaceutical Sciences, Toho University, Chiba, Japan.
Goto
S
S
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001057:Apolipoproteins E / Q000235:genetics; D015153:Blotting, Western; D002478:Cells, Cultured; D008099:Liver / Q000201:enzymology*; D008297:Male; D008668:Metallothionein / Q000235:genetics; D008970:Molecular Weight; D011228:Prealbumin / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D015183:Restriction Mapping; D014018:Tissue Distribution; D014503:Urate Oxidase / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D001057:Apolipoproteins E; D011228:Prealbumin; D012333:RNA, Messenger; D008668:Metallothionein; D014503:Urate Oxidase