Possible involvement of the ugpA gene product in the stable maintenance of mini-F plasmid in Escherichia coli.
The seg-3 mutant Escherichia coli does not support the maintenance of mini-F plasmid at 42 degrees C. We cloned the chromosomal DNA segment of the wild-type strain W3110 that complements the Seg- phenotype of this mutant. Cleavage mapping of this segment showed that it was derived from the 76-min region of the E. coli chromosome map. Complementation tests using plasmids carrying subcloned DNA segments suggested that the seg-3 mutant carried two mutations that additively affected the maintenance of mini-F plasmid; one was in the ugpA gene and the other was presumably in the rpoH gene. We generated a disrupted ugpA null mutant and found that the mini-F plasmid was unstable in this ugpA null mutant even at 30 degrees C. This suggests that the ugpA gene product is required for the stable maintenance of mini-F plasmid.
Ezaki
B
B
Department of Molecular Genetics, Kumamoto University Medical School, Japan.
Mori
H
H
Ogura
T
T
Hiraga
S
S
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001692:Biological Transport; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D005144:F Factor; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D006360:Heat-Shock Proteins / Q000378:metabolism; D009154:Mutation; D010641:Phenotype; D010710:Phosphates / Q000378:metabolism; D015183:Restriction Mapping
D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D010710:Phosphates