Specific plate assay for bacterial heparinase.
A procedure was developed for detecting heparinase activity on heparin agar plates. The method is based on the differential precipitation of heparin and heparinase-generated heparin fragments by protamine sulfate. Heparinase activity is detected by the presence of clear zones against a white background. This method can be used to screen for the expression of recombinant heparinase and to identify Flavobacterium heparinum mutants expressing heparinase constitutively.
Zimmermann
J J
JJ
IBEX Technologies, Montreal, Quebec, Canada.
Langer
R
R
Cooney
C L
CL
D000362:Agar; D005417:Flavobacterium / Q000201:enzymology* / Q000235:genetics; D006493:Heparin; D019763:Heparin Lyase; D008722:Methods; D009154:Mutation; D011133:Polysaccharide-Lyases / Q000032:analysis* / Q000235:genetics; D011479:Protamines
D011479:Protamines; D000362:Agar; D006493:Heparin; D011133:Polysaccharide-Lyases; D019763:Heparin Lyase