Radiolabeling of Necator americanus third stage larvae with 75Se-methionine.
Third stage larvae of Necator americanus were radiolabeled with 75Se-methionine by two methods. Larvae labeled in aqueous cultures contained 188 and 25 cpm per sheathed and ex-sheathed larva, respectively. Larvae labeled in coproculture incorporated 25 and 18 cpm per sheathed and ex-sheathed larva, respectively. All of the label was decayed in 5 days from larvae labeled in aqueous cultures, whereas appreciable amounts of radioactivity were still detectable at day 7 of chase period in coproculture labeled larvae.
Siddiqui
A A
AA
Department of Biomedical Sciences, University of Illinois, College of Medicine, Rockford 61107.
Lei
Y
Y
Fusco
A C
AC
Salafsky
B
B
D000818:Animals; D007553:Isotope Labeling; D007814:Larva / Q000378:metabolism; D008715:Methionine / Q000378:metabolism*; D009331:Necator / Q000378:metabolism*; D012644:Selenium Radioisotopes
D012644:Selenium Radioisotopes; D008715:Methionine