[Trypsin and alpha-amylase activities in the pancreas of guinea pigs. III. Effect of homogenates of invasive ova of Ascaris suum and organic acids C2-C6 on the activity of enzymes in pancreatic extracts].
The studies were carried out on pancreas extracts from guinea pigs free of parasite invasion. The activity of trypsin was determined according to the method of Anson, and amylase of Fennel method. The measurements of activity were done at the presence of homogenized invasive eggs of Ascaris suum, and the mixture of volatile organic acids C2-C6 (at concentrations of 30.3 and 0.3 mM/l). In samples with homogenized invasive eggs of A. suum the amylase activity remained unchanged while the activity of trypsin was considerably higher (p less than 0.01). The introduction of the organic acids at conc. 30 mM/l into the samples was the reason of lower amylase activity. No influence of these acids on trypsin activity in pancreas extracts was observed.
Zółtowska
K
K
Zakład Cytologii i Genetyki, Instytut Biologii WSP.
D000818:Animals; D001196:Ascariasis / Q000201:enzymology*; D001200:Ascaris; D004195:Disease Models, Animal; D005232:Fatty Acids, Volatile / Q000494:pharmacology*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D010179:Pancreas / Q000201:enzymology*; D010184:Pancreatic Extracts / Q000737:chemistry*; D014020:Tissue Extracts / Q000494:pharmacology*; D014357:Trypsin / Q000737:chemistry / Q000378:metabolism*; D000516:alpha-Amylases / Q000737:chemistry / Q000378:metabolism*
D005232:Fatty Acids, Volatile; D010184:Pancreatic Extracts; D014020:Tissue Extracts; D000516:alpha-Amylases; D014357:Trypsin
Aktywność trypsyny i alfa-amylazy w trzustce świnek morskich. III. Wpływ homogenatów z inwazyjnych jaj ascaris suum oraz mieszaniny kwasów organicznych C2-C6 na aktywność enzymów w wyciagach z trzustki.