Catenin association with E-cadherin changes with the state of polarity of HT-29 cells.
Cadherins are transmembrane glycoproteins that mediate the calcium-dependent adhesion of cells to one another. It has been reported that at least two and probably more proteins associate with cadherins in various systems. These proteins have been called catenins. HT-29 cells can be manipulated to express either a polar or a nonpolar phenotype, depending on the growth conditions. We have taken advantage of this feature of HT-29 cells to explore the role catenins may play in cadherin-mediated adhesion. In this paper we report that several catenins co-immune-precipitate with E-cadherin in cultured human cells (HT-29 and JAR PR497) and that the nature of the complex of proteins varies with the physiological state of the HT-29 cells. In addition, we show data suggesting that the proteins that associate with calcium-dependent adhesion molecules may represent a group of proteins, some of which are present in all cells and some of which are cell-type specific.
Wheelock
M J
MJ
Department of Biology, University of Toledo, Ohio 43606.
D015153:Blotting, Western; D015820:Cadherins / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D002822:Choriocarcinoma / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D004848:Epithelium / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D011233:Precipitin Tests; D011247:Pregnancy; D011506:Proteins / Q000378:metabolism* / Q000502:physiology; D014594:Uterine Neoplasms / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology
D015820:Cadherins; D011506:Proteins