title: Functional similarity between the haemolysins of Escherichia coli and Morganella morganii.
abstract: Haemolysin produced by a clinical isolate of Morganella morganii was examined for antigenic relatedness to the haemolysin of Escherichia coli and for similarities in mode of action. The M. morganii haemolysin migrated in SDS-PAGE as a single protein band with a slightly higher molecular weight than that of E. coli haemolysin. Several murine monoclonal antibodies against E. coli haemolysin cross-reacted with the M. morganii haemolysin in Western blots. Diminished haemolysis in the presence of osmotically-stabilising solutes indicated the formation of a pore by M. morganii haemolysin with an effective diameter of 1.5-3 nm. Results from dose-response experiments indicated that a single "hit" was sufficient for lysis of an erythrocyte. Detergent solubilisation of toxin-treated membranes led to recovery of bound toxin exclusively in monomeric form. M. morganii haemolysin was a potent leucocidin, that caused rapid leakage of ATP and death of human polymorphonuclear leucocytes. Under in-vitro conditions M. morganii haemolysin displayed similar leucocidal and haemolytic efficiency. The data demonstrate that M. morganii haemolysin shows functional properties virtually identical with those of E. coli haemolysin.
authors: Institute of Medical Microbiology, University of Giessen, West Germany.
authors: B
authors: B
authors: Eberspächer
authors: F
authors: F
authors: Hugo
authors: M
authors: M
authors: Pohl
authors: S
authors: S
authors: Bhakdi
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis; D015153:Blotting, Western; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000502:physiology*; D006461:Hemolysis; D006801:Humans; D066298:In Vitro Techniques; D009504:Neutrophils / Q000378:metabolism / Q000382:microbiology; D009996:Osmotic Fragility; D011511:Proteus / Q000378:metabolism*
chemical_list: D006460:Hemolysin Proteins; D000255:Adenosine Triphosphate