title: Specific plate assay for bacterial heparinase.
abstract: A procedure was developed for detecting heparinase activity on heparin agar plates. The method is based on the differential precipitation of heparin and heparinase-generated heparin fragments by protamine sulfate. Heparinase activity is detected by the presence of clear zones against a white background. This method can be used to screen for the expression of recombinant heparinase and to identify Flavobacterium heparinum mutants expressing heparinase constitutively.
authors: IBEX Technologies, Montreal, Quebec, Canada.
authors: J J
authors: JJ
authors: Zimmermann
authors: R
authors: R
authors: Langer
authors: C L
authors: CL
authors: Cooney
mesh_terms: D000362:Agar; D005417:Flavobacterium / Q000201:enzymology* / Q000235:genetics; D006493:Heparin; D019763:Heparin Lyase; D008722:Methods; D009154:Mutation; D011133:Polysaccharide-Lyases / Q000032:analysis* / Q000235:genetics; D011479:Protamines
chemical_list: D011479:Protamines; D000362:Agar; D006493:Heparin; D011133:Polysaccharide-Lyases; D019763:Heparin Lyase