Preparation of protoplasts and regeneration of intact cells of Streptomyces cinnamonensis.
Protoplasts were prepared and intact cells were regenerated in Streptomyces cinnamonensis--a monensin producer--to make genetic manipulations with this strain possible. 70-80% of protoplasts were formed and up to 90% of them could regenerate into intact cells.
Jandová
Z
Z
Department of General Microbiology, Czechoslovak Academy of Sciences, Prague.
Tichý
P
P
D003470:Culture Media; D011523:Protoplasts / Q000502:physiology*; D013302:Streptomyces / Q000502:physiology*
D003470:Culture Media