Influence of DNA repair deficiencies on the UV sensitivity of yeast cells in different cell cycle stages.
Synchronously dividing haploid yeast cells were UV-irradiated in various stages of the cell cycle after release from alpha-factor arrest. In confirmation of earlier results (Chanet et al., 1973), in wild-type strains G1/S phase cells were found to be the most sensitive and late S/G2 cells the most resistant. Stationary-phase (G0) cells were significantly more UV resistant than G1 cells. Strains defective in nucleotide excision repair lost enhanced resistance in the G2 phase and were most UV-sensitive in the G0 state. Reduced G2 resistance was also observed in rad6 mutants but not in rad9 mutants. After UV-irradiation in G1 phase rad9 mutant cells showed a reduced G1/S phase arrest.
Siede
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Department of Pathology, Stanford University School of Medicine, CA 94305.
Friedberg
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EC
D002453:Cell Cycle / Q000528:radiation effects; D004260:DNA Repair; D004271:DNA, Fungal / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D006238:Haploidy; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects; D014466:Ultraviolet Rays
D004271:DNA, Fungal