Genomic analysis of a mouse zinc finger gene, Zfp-35, that is up-regulated during spermatogenesis.
Zinc finger genes are a class of eukaryotic regulatory genes that encode sequence-specific nucleic acid-binding proteins. Members of this large gene family are required for growth and development in a wide range of organisms. We previously identified a mouse zinc finger gene, Zfp-35, that was up-regulated during spermatogenesis at the pachytene spermatocyte stage of development. We now describe the genomic organization of this gene, including its intron-exon structure, the sequence of its flanking regions, and its assignment to a region encompassing bands B3 to C of chromosome 18. The transcription unit has three exons. Intron 1 is within the 5' untranslated region and exon 3 contains the block of all 18 zinc fingers. These two features are common to a number of zinc finger genes. We also show that Zfp-35 is conserved in some placental mammals and that it is a member of a subfamily of related mammalian zinc finger genes.
Cunliffe
V
V
Imperial Cancer Research Fund Laboratories, London, United Kingdom.
Williams
S
S
Trowsdale
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004268:DNA-Binding Proteins / Q000235:genetics*; D005809:Genes, Regulator; D008297:Male; D008322:Mammals / Q000235:genetics; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D009928:Organ Specificity; D013091:Spermatogenesis; D016335:Zinc Fingers / Q000235:genetics*
D004268:DNA-Binding Proteins