Structural analysis of the rabbit TNF locus, containing the genes encoding TNF-beta (lymphotoxin) and TNF-alpha (tumor necrosis factor).
The genes coding for 20-kDa lymphotoxin (TNF-beta) and tumor necrosis factor (TNF-alpha) have been cloned from a rabbit genomic library. The two genes are tandemly arranged and separated by only 1 kb of DNA, as previously observed in human and mouse genomes. We have sequenced the entire rabbit lymphotoxin-encoding gene and inferred the primary structure of rabbit TNF-beta, whose cDNA is not yet cloned. We also analysed the upstream sequences of the rabbit TNF-beta and TNF-alpha genes and identified a number of potential binding sites for known nuclear transcription factors, and in particular several putative kappa B-type sequences.
Shakhov
A N
AN
Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
Kuprash
D V
DV
Azizov
M M
MM
Jongeneel
C V
CV
Nedospasov
S A
SA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D016384:Consensus Sequence; D015723:Gene Library; D005796:Genes; D006801:Humans; D008233:Lymphotoxin-alpha / Q000235:genetics*; D008969:Molecular Sequence Data; D011817:Rabbits; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D016415:Sequence Alignment; D014157:Transcription Factors / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D008233:Lymphotoxin-alpha; D014157:Transcription Factors; D014409:Tumor Necrosis Factor-alpha