1 Alpha-hydroxyvitamin D3 suppresses colonic tumorigenesis induced by repetitive intrarectal injection of N-methyl-N-nitrosourea in rats.
The effect of 1 alpha-hydroxyvitamin D3 (1 alpha (OH)D3) on colonic tumorigenesis induced by chronic treatment with N-methyl-N-nitrosourea (MNU) was studied in rats. Seventy-four female F344 rats received an intrarectal injection of 1 mg of MNU once a week for 40 weeks. Two-thirds of rats were given concomitant administration of 0.2 ml of medium chain triglyceride (MCT) or MCT containing 0.04 microgram of 1 alpha (OH)D3 through an intragastric route thrice weekly. Numbers of rats bearing colonic tumor were 21 in MNU alone (n = 24), 17 in MNU + MCT (n = 25) and 12 in MNU + 1 alpha (OH)D3 group (n = 25) (uncorrected chi 2 = 8.72). The result indicated that colonic tumorigenesis induced by the chronic treatment with MNU was suppressed by oral supplementation of 1 alpha (OH)D3 and the inhibitory effect of 1 alpha (OH)D3 was partly due to the effect of MCT.
Kawaura
A
A
Department of Internal Medicine Hyogo College of Medicine, Japan.
Takahashi
A
A
Tanida
N
N
Oda
M
M
Sawada
K
K
Sawada
Y
Y
Maekawa
S
S
Shimoyama
T
T
D000236:Adenoma / Q000139:chemically induced / Q000188:drug therapy / Q000453:epidemiology; D000285:Administration, Rectal; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000188:drug therapy* / Q000453:epidemiology; D005260:Female; D006887:Hydroxycholecalciferols / Q000494:pharmacology*; D015994:Incidence; D008770:Methylnitrosourea / Q000008:administration & dosage; D051381:Rats; D011916:Rats, Inbred F344
D006887:Hydroxycholecalciferols; D008770:Methylnitrosourea; C008088:alfacalcidol