Lewis typing of human saliva stains by enzyme-linked immunosorbent assay (ELISA) using monoclonal anti-Le(a) and anti-Le(b) antibodies.
The Lewis blood grouping of human saliva stains could be detected by an enzyme-linked immunosorbent assay (ELISA) using anti-Le(a) and anti-Le(b) monoclonal antibodies with an avidin-biotin complex (ABC). The saliva stains (1.0 by 1.0 cm in size) were used as samples and not only could the Lewis substances of 57 individual stains be correctly typed by this method, but also it was clarified that there are several different secretion patterns of amounts of Le(a) and Le(b) substances in 3 individual Lewis types.
Takatori
T
T
Department of Legal Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
Tsutsubuchi
Y
Y
Terazawa
K
K
Nagao
M
M
Akabame
H
H
Mikami
H
H
D000911:Antibodies, Monoclonal / Q000276:immunology*; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000276:immunology*; D012463:Saliva / Q000276:immunology*
D000911:Antibodies, Monoclonal; D007983:Lewis Blood Group Antigens