Oxidation of coniferyl alcohol by cell wall peroxidases at the expense of indole-3-acetic acid and O2. A model for the lignification of plant cell walls in the absence of H2O2.
The oxidation of coniferyl alcohol (CA), a lignin precursor, by cell wall peroxidases may take place at the expense of indole-3-acetic acid (IAA) and O2, and in the absence of H2O2. The peroxidase-catalyzed oxidation of CA shows an optimum at an IAA concentration of 0.33 mM, while higher IAA concentrations are inhibitory. The observation that the oxidation of CA by cell wall peroxidase at the expense of IAA and O2 is inhibited by genistein, a putative endogenous inhibitor of lignification in lupin hypocotyls, supports the view that the H2O2-generating system coexists with cell wall peroxidase activities involved in lignification, and that it takes place at the expense of IAA and O2.
Ferrer
M A
MA
Department of Plant Biology (Plant Physiology), University of Murcia, Spain.
Pedreño
M A
MA
Muñoz
R
R
Barceló
A R
AR
D002473:Cell Wall / Q000201:enzymology; D007210:Indoleacetic Acids / Q000378:metabolism*; D007700:Kinetics; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D010544:Peroxidases / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism*; D010944:Plants / Q000201:enzymology*; D013056:Spectrophotometry, Ultraviolet; D013379:Substrate Specificity
D007210:Indoleacetic Acids; D010636:Phenols; C030737:indoleacetic acid; C010559:coniferyl alcohol; D010544:Peroxidases