Isolation and characterization of cell hybrids containing human Xp-chromosome fragments.
We have subjected C12D, a Chinese hamster hybrid containing only the human X chromosome, to 6-thioguanine selection. The majority of the derivative clones retained rearranged Xp-fragments, which were characterized by using a combination of enzyme markers, DNA probes, and in situ hybridization. Two of these, TG2 and TG5sc9.1, contained only an Xpter----p21 fragment and should be an ideal resource for directed cloning from this region. A possible mechanism for the specific retention of Xp-fragments is discussed.
Wapenaar
M C
MC
Department of Human Genetics, Sylvius Laboratory, State University of Leiden, The Netherlands.
Kievits
T
T
Meera Khan
P
P
Pearson
P L
PL
Van Ommen
G J
GJ
D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D002874:Chromosome Mapping; D006224:Cricetinae; D015342:DNA Probes; D004351:Drug Resistance / Q000235:genetics; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D007621:Karyotyping; D013866:Thioguanine / Q000494:pharmacology; D014960:X Chromosome
D015342:DNA Probes; D005819:Genetic Markers; D007041:Hypoxanthine Phosphoribosyltransferase; D013866:Thioguanine