An evaluation of the CHO/HGPRT mutation assay involving suspension cultures and soft agar cloning: results for 33 chemicals.
The Chinese hamster ovary cell assay (CHO), which measures forward mutation of the HGPRT locus, is used in several laboratories for the detection of mutagens. A procedure involving treatment of CHO cells in suspension culture and mutant selection in soft agar cloning has been developed (Oberly TJ, Bewsey BJ, Probst GS (1987): Mutat Res 182:99-111). In order to evaluate the effectiveness of these modifications, 33 chemicals representing six chemical classes were tested, and the results were compared to findings obtained in other tests for genotoxicity at Lilly Research Laboratories (LRL). A positive response was obtained with 21 chemicals, all of which are recognized mutagens. Of the 12 compounds that produced negative results, 4 were considered to be mutagens and/or carcinogens. Twelve of the compounds mentioned in this report have been previously tested in the CHO/HGPRT assay by other laboratories, and the results showed strong agreement between laboratories. These findings support the conclusion that the use of suspension cultures and soft agar cloning in the CHO assay provides a sensitive test for the identification of mutagens and is a viable alternative to the traditional monolayer procedure of O'Neill et al. (O'Neill JP, Couch DB, Machanoff R, San Sebastian JR, Brimer PA, Hsie AW (1977): Mutat Res 45:103-109).
Oberly
T J
TJ
Toxicology Division, Lilly Research Laboratories, Greenfield, Indiana 46140.
Rexroat
M A
MA
Bewsey
B J
BJ
Richardson
K K
KK
Michaelis
K C
KC
D000362:Agar; D000818:Animals; D001711:Biotransformation; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002999:Clone Cells; D006224:Cricetinae; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics* / Q000378:metabolism; D008862:Microsomes, Liver; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000032:analysis*
D009153:Mutagens; D000362:Agar; D007041:Hypoxanthine Phosphoribosyltransferase