[Detection of verotoxin-producing E. coli (VTEC) in healthy cattle and swine with the DNA-DNA colony hybridization method].
With the DNA-DNA colony hybridization technique using specific gene probes for Verotoxin 1 (VT 1) and Verotoxin 2 (VT 2) 2100 E. coli strains from healthy animals were tested. Ten out of 82 milk cows (21.2%), 20 out of 212 beef cattle (9.4%) and five out of 75 pigs (6.7%) were found to carry genes for VT 1, VT 2 or both toxins, respectively. Among these strains the biotypes 5 and 6 were predominant. Some of the serotyped isolates have been described to be pathogenic for humans, like O157:H7, 082:H8, 0116, 0113, 0126 and 091, respectively. The unexpected high incidence of VTEC positive healthy animals possibly indicates a health hazard for human beings. Further investigations on the incidence of VTEC in food are necessary.
Bülte
M
M
Institut für Fleischhygiene der Freien Universität Berlin.
Montenegro
M A
MA
Helmuth
R
R
Trumpf
T
T
Reuter
G
G
D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis*; D002417:Cattle / Q000382:microbiology*; D003603:Cytotoxins / Q000096:biosynthesis; D004269:DNA, Bacterial / Q000032:analysis*; D004768:Enterotoxins / Q000096:biosynthesis; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D008297:Male; D009693:Nucleic Acid Hybridization; D022622:Shiga Toxin 1; D013552:Swine / Q000382:microbiology*
D001427:Bacterial Toxins; D003603:Cytotoxins; D004269:DNA, Bacterial; D004768:Enterotoxins; D022622:Shiga Toxin 1
Nachweis von Verotoxin-bildenden E. coli (VTEC) bei gesunden Rindern und Schweinen mit dem DNS-DNS-Koloniehybridisierungsverfahren.