Exudation induces clustering of CR1 receptors at the surface of human polymorphonuclear leukocytes.
The complement receptor type 1 (CR1) surface distribution, density and immune adherence efficiency were determined in circulating PMN activated by fMLP, NAP-1/IL-8, TNF, GM-CSF and C5a, or exudate PMN harvested from skin-blisters. These observations were compared with those observed on resting peri-pheral blood PMN. PMN activators known to upregulate CR1 expression did not induce a significant increase in CR1 clustering, or immune adherence efficiency towards opsonized immune complexes. By contrast, increase in CR1 density at the surface of exudated PMN was accompanied by an increased clustering. This clustering was however insufficient to increase the binding efficiency for immune complexes. Eventually, CR1 expression of exudated neutrophil could not be increased further by stimulation with fMLP or PMA. These results indicated that clustering of CR1 on PMN may occur in vivo. Such reaction might determine the phagocytic potential of the cell for opsonized micro-organisms or debris. This clustering could not be attributed to one of the PMN activators tested.
Paccaud
J P
JP
Institute of Histology and Embryology, University Medical Center, Geneva, Switzerland.
Carpentier
J L
JL
Schifferli
J A
JA
D003172:Complement C1 / Q000378:metabolism*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D016209:Interleukin-8 / Q000494:pharmacology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000378:metabolism*; D011951:Receptors, Complement / Q000187:drug effects / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D003172:Complement C1; D016209:Interleukin-8; D011951:Receptors, Complement; D014409:Tumor Necrosis Factor-alpha; D009240:N-Formylmethionine Leucyl-Phenylalanine; D016178:Granulocyte-Macrophage Colony-Stimulating Factor