Uterine secretions of the cow contain proteins that are immunochemically related to the major progesterone-induced proteins of the sheep uterus.
Proteins that cross-react with antiserum to the major progesterone-induced proteins found in the pregnant sheep uterus, the uterine milk proteins (UTM-proteins), were detected as radiolabelled secretory products of endometrium from pregnant cows. Cross-reactive proteins included a form at 57,000 molecular weight as well as other lower-molecular-weight variants found in lower amounts. Similar proteins were also detected in uterine fluid from a cow at day 270 of gestation. Using immunohistochemical procedures, proteins that cross-reacted with antiserum to sheep UTM-proteins could be localized to the epithelial cells of endometrial glands in the cow. Results indicate that UTM-protein-like molecules are secreted by the endometrium of the cow during pregnancy.
Leslie
M V
MV
Dairy Science Department, University of Florida, Gainesville 32611-0701.
Hansen
P J
PJ
Newton
G R
GR
D000818:Animals; D015153:Blotting, Western; D002417:Cattle / Q000378:metabolism*; D003429:Cross Reactions; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D006023:Glycoproteins; D007150:Immunohistochemistry; D011233:Precipitin Tests; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000378:metabolism*; D011374:Progesterone / Q000502:physiology; D011506:Proteins / Q000378:metabolism*; D015843:Serpins; D012756:Sheep / Q000378:metabolism*
D006023:Glycoproteins; D011257:Pregnancy Proteins; D011506:Proteins; D015843:Serpins; C029708:uterine luminal fluid proteins; D011374:Progesterone