[Study of HDL2-dependent synthesis of bile acids in culture of rabbit hepatocytes: effects of oxidized cholesterol derivatives].
The effect of individual oxysterols--products of auto-oxidation of cholesterol on bile acid synthesis by cultivated rabbit hepatocytes was studied. Relative rates of bile acid synthesis were measured as the conversion of 4-14C cholesterol-HDL2 into total 4-14C labeled bile acids. 7 beta-hydroxycholesterol and 3,5-cholestane-7-dione strongly inhibited bile acid synthesis at concentrations 1-10 micrograms/ml. These data support the hypothesis that oxidized cholesterol derivatives accelerate the development of hypercholesterolemia in rabbits fed on cholesterol containing diet.
Novikov
D K
DK
Kosykh
V A
VA
Antonov
I V
IV
Lakeev
Iu V
IuV
Repin
V S
VS
D000818:Animals; D001647:Bile Acids and Salts / Q000096:biosynthesis*; D002478:Cells, Cultured; D002778:Cholestanones / Q000494:pharmacology; D002791:Cholesterol, Dietary / Q000494:pharmacology; D008076:Cholesterol, HDL / Q000378:metabolism*; D006888:Hydroxycholesterols / Q000494:pharmacology; D006937:Hypercholesterolemia / Q000209:etiology; D008075:Lipoproteins, HDL / Q000378:metabolism*; D053439:Lipoproteins, HDL2; D008079:Lipoproteins, VLDL / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D011817:Rabbits
D001647:Bile Acids and Salts; D002778:Cholestanones; D002791:Cholesterol, Dietary; D008076:Cholesterol, HDL; D006888:Hydroxycholesterols; D008075:Lipoproteins, HDL; D053439:Lipoproteins, HDL2; D008079:Lipoproteins, VLDL; C011724:cholest-5-en-3 beta,7 alpha-diol
Izuchenie LVP2-zavisimogo sinteza zhelchnykh kislot v kul'ture gepatotsitov krolika: éffekty oksiproizvodnykh kholesterina.