Characterization of 5B12.1, a monoclonal antibody specific for IL-6.
A monoclonal antibody (MAb) specific for interleukin-6 (IL-6) was generated by fusing SP2/0 cells with spleen cells from a mouse immunized with rat spleen cell derived plasmacytoma growth factor (rat PCT-GF). This MAb inhibited the growth of an IL-6-sensitive murine plasmacytoma clone, MD90, in the presence of the immunogen, rat PCT-GF. More interesting, however, this MAb demonstrated species cross-reactivity by neutralizing murine (recombinant and P388D1 cell line-derived) and human (recombinant) IL-6. IL-6 neutralization activity was also established in other IL-6 bioassays, such as the proliferation of spleen cells, plasmacytoma T1165, and a B-cell hybridoma 7TD1. IL-6 neutralization was overcome partially by increasing the concentration of PCT-GF. The MAb had no effect on PCT-GF-independent plasmacytoma KI81 proliferation. Plastic petri dish-bound MAb removed rmIL-6 activity. These results suggest that this MAb specifically binds IL-6 and neutralizes bioactivity of various PCT-GF, rmIL-6, and rhIL-6.
Sawamura
M
M
Department of Microbiology, University of Alabama, Birmingham.
Everson
M P
MP
Shrestha
K
K
Ghanta
V K
VK
Miller
D M
DM
Hiramoto
R N
RN
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002455:Cell Division; D002478:Cells, Cultured; D003429:Cross Reactions; D006801:Humans; D006825:Hybridomas; D015850:Interleukin-6 / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D010954:Plasmacytoma; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000166:cytology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015850:Interleukin-6