Northern blot analysis of HLA-G expression by BeWo human choriocarcinoma cells.
The BeWo human choriocarcinoma cell line expresses an unusual 41 kDa beta 2-microglobulin-associated class I MHC antigen. HLA-E, -F and -G are known to encode antigens of lower Mr than HLA-A, -B or -C proteins. Using locus-specific probes, we now show that RNA isolated from BeWo cells contains markedly more HLA-G transcripts than peripheral blood lymphocytes (PBL), whilst the converse was noted for HLA-B. These results support recent cloning and sequencing data showing the presence of HLA-G transcripts in BeWo cells.
Risk
J M
JM
Department of Immunology, University of Liverpool, U.K.
Johnson
P M
PM
D015152:Blotting, Northern; D002822:Choriocarcinoma / Q000235:genetics / Q000276:immunology*; D005260:Female; D006680:HLA Antigens / Q000235:genetics*; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D011247:Pregnancy; D012334:RNA, Neoplasm / Q000235:genetics; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured / Q000276:immunology; D014594:Uterine Neoplasms / Q000235:genetics / Q000276:immunology*
D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; D012334:RNA, Neoplasm