[Induction by Staphylococcus aureus L-form of tumor necrosis factor-alpha from macrophages].
Induction of tumor necrosis factor-alpha (TNF-alpha) by Staphylococcus aureus L-form was investigated. The supernatant of a macrophage culture mixed with S. aureus L-form showed a potent cytotoxic activity to L cells. Addition of anti TNF-alpha antibody inhibited completely the cytotoxic activity of the supernatant, indicating that the activity might be due mostly to TNF-alpha. To investigate localization of TNF-alpha production, the membranes of hypotonicity treated L-form were layered on a step-gradient composed of an upper and lower layers of 35% and 50% sucrose, respectively. The membranes were banded at the interface of 35% and 50% of sucrose. The activity of TNF-alpha production of the membrane fraction was 10-times higher than that of the soluble fraction.
Matsu-Ura
I
I
Department of Microbiology, Kurume University School of Medicine, Japan.
Kuwano
K
K
Umeda
A
A
Amako
K
K
Yamamoto
Y
Y
Arai
S
S
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D007740:L Forms / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D013211:Staphylococcus aureus / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000502:physiology
D014409:Tumor Necrosis Factor-alpha