[Explanation of nonspecific serologic reactions in decomposed bone tissue].
Bone fragments were stored in water for 2 years at room temperature and investigated serologically using the following methods: Absorption-elution, extraction of blood group substances and immunohistochemistry (PAP). All 3 methods gave essentially specific results for fresh bone tissue but with putrid bone tissue unspecific reactions were found predominantly with the absorption-elution and PAP techniques. In contrast, more specific reactions were obtained from the extracts although they were much weaker. From this it can be concluded that pure physical binding plays a substantial role in the unspecific reaction between antibodies and bone material. It is suggested that the relevant physical properties are altered by putrification.
Hauser
R
R
Institut für Rechtsmedizin, Medizinische Akademie Gdánsk, Polen.
Brinkmann
B
B
D000017:ABO Blood-Group System / Q000235:genetics*; D001842:Bone and Bones / Q000737:chemistry / Q000473:pathology*; D006801:Humans; D007124:Immunoenzyme Techniques; D010641:Phenotype; D011180:Postmortem Changes
D000017:ABO Blood-Group System
Beitrag zur Erklärung unspezifischer serologischer Reaktionen in fauligem Knochengewebe.