title: Metabolism of phosphatidylglycerol and bis(monoacylglycero)-phosphate in macrophage subcellular fractions.
abstract: Bis(monoacylglycero)phosphate (BMP) is synthesized from exogenous phosphatidylglycerol (PG) by macrophages (Cochran, F. R., Roddick, V. L., Connor, J. R., Thornburg, J. T., and Waite, M. (1987) J. Immunol. 138, 1877-1883). Previous work from our laboratory showed that arachidonic acid in BMP was released by the macrophages upon challenge of the cells with PMA (Cochran, F. R., Connor, J. R., Roddick, V. L., and Waite, M. (1985) Biochem. Biophys. Res. Commun. 130, 800-806). Here we extend those studies using a model cultured cell line of macrophages, RAW 264.7. When PG labeled with 32P- and [3H]glycerol in both moieties was added to the culture medium, 32P/[3H]BMP was synthesized in a time-dependent manner. Fractionation of cell homogenates on a discontinuous sucrose gradient in which the light membranes were floated from dense sucrose showed an enrichment of [3H]BMP in light membrane fractions. The precursor [3H]PG was also found in the light fractions but, relative to the [3H]BMP, was more abundant in the denser membrane fractions. The appearance of [3H]PG and [3H]BMP in the light membrane fraction was time-dependent which suggested that the initial uptake and metabolism of [3H]PG was into the denser membranes. Incubation of the light membranes under conditions that are optimal for the lysosomal phospholipase A1 led to significant metabolism of [3H]PG. Both degradation of [3H]PG to water-soluble compounds and its conversion to acylphosphatidylglycerol occurred while no lyso-PG was detected. On the other hand, little BMP was found to be degraded. From these studies we postulate that in lysosomes acylphosphatidylglycerol is a precursor of BMP and that the previously reported turnover of arachidonic acid by BMP may occur via transacylation rather than hydrolysis.
authors: Department of Biochemistry, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, North Carolina 27103.
authors: M
authors: M
authors: Waite
authors: L
authors: L
authors: King
authors: T
authors: T
authors: Thornburg
authors: G
authors: G
authors: Osthoff
authors: T Y
authors: TY
authors: Thuren
mesh_terms: D000818:Animals; D002460:Cell Line; D066298:In Vitro Techniques; D008246:Lysophospholipids / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism; D008566:Membranes / Q000378:metabolism; D051379:Mice; D050178:Monoglycerides; D009113:Muramidase / Q000378:metabolism; D009245:NADH Dehydrogenase / Q000378:metabolism; D010715:Phosphatidylglycerols / Q000378:metabolism*; D010740:Phospholipases / Q000378:metabolism; D013329:Structure-Activity Relationship; D013347:Subcellular Fractions / Q000201:enzymology / Q000378:metabolism
chemical_list: D008246:Lysophospholipids; D008563:Membrane Lipids; D050178:Monoglycerides; D010715:Phosphatidylglycerols; C012786:bis(monoacylglyceryl)phosphate; D009245:NADH Dehydrogenase; D010740:Phospholipases; D009113:Muramidase