[The detection of the pathogenicity of Listeria using permanent cell lines as an alternative for animal studies].
Culture filtrates of 38 strains of Listeria (L.) monocytogenes and of 4 strains of L. ivanovii were all cytotoxic for the vero cell line. Culture filtrates from 35 strains of L. innocua, 12 strains of L. seeligeri, 5 strains of L. welshimeri and 4 strains of L. grayi showed no cytotoxicity for the continuous cell line vero. The use of continuous cell lines permits to distinguish between pathogenic and non-pathogenic Listeria strains and seems to be a suitable method to replace the mouse pathogenicity test or the fertilized hen's eggs test.
Heil
G
G
Landesuntersuchungsamt für das Gesundheitswesen Nordbayern, Nürnberg.
Weber
A
A
D000826:Animal Testing Alternatives; D000818:Animals; D008087:Listeria / Q000472:pathogenicity*; D014709:Vero Cells
Zum Nachweis der Pathogenität von Listerien mittels permanenter Zellinie als Alternative für den Tierversuch.