title: Sulphation of hirudin in BHK cells.
abstract: Hirudin, a thrombin inhibitor of the leech, was expressed in BHK cells; the alpha 1-antitrypsin signal peptide was used to direct secretion into the culture medium. The recombinant hirudin so produced inhibited thrombin and was shown by labelling experiments with [35S]sulphate to have been posttranslationally modified.
authors: Transgene SA, Strasbourg, France.
authors: T
authors: T
authors: Skern
authors: R
authors: R
authors: Bischoff
authors: S
authors: S
authors: Jallat
authors: K
authors: K
authors: Dott
authors: D
authors: D
authors: Ali-Hadji
authors: D
authors: D
authors: Clesse
authors: M P
authors: MP
authors: Kieny
authors: M
authors: M
authors: Courtney
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D006224:Cricetinae; D004252:DNA Mutational Analysis; D015870:Gene Expression; D006629:Hirudins / Q000378:metabolism*; D066298:In Vitro Techniques; D007668:Kidney; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals; D011994:Recombinant Proteins / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism*; D013917:Thrombin / Q000037:antagonists & inhibitors
chemical_list: D006629:Hirudins; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D013431:Sulfates; D013917:Thrombin