Identification of two types of neurophysins in Xenopus laevis neurointermediate pituitary homologous to mammalian MSEL- and VLDV-neurophysins.
Xenopus laevis neurophysins have been purified from neurointermediate pituitaries through high-pressure reverse-phase liquid chromatography and their N-terminal amino acid sequences have been determined by microsequencing. Two types of neurophysins, corresponding to mammalian MSEL- and VLDV-neurophysins, have been distinguished. A strong homology exists between neurophysins of Xenopus (Pipidae), frog (Ranidae) and toad (Bufonidae). Xenopus MSEL-neurophysin, as frog MSEL-neurophysin, has a high molecular mass suggesting that the C-terminal domain of the vasotocin precursor is not processed in contrast to the two-step processing observed for mammalian vasopressin precursor.


BACKGROUND
Mammalian neurophysins are termed MSEL- and VLDV-neurophysins according to the nature of residues in positions 2, 3, 6 and 7 (one-letter symbols for amino acids).
Chauvet
J
J
Laboratory of Biological Chemistry, University of Paris VI 96, France.
Michel
G
G
Rouillé
Y
Y
Chauvet
M T
MT
Acher
R
R
D000595:Amino Acid Sequence; D000818:Animals; D002025:Bufonidae; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D009481:Neurophysins / Q000737:chemistry / Q000302:isolation & purification*; D010902:Pituitary Gland / Q000737:chemistry*; D011893:Rana esculenta; D012689:Sequence Homology, Nucleic Acid; D014982:Xenopus laevis
D009481:Neurophysins