title: Experimental ocular onchocerciasis: local and systemic antibody and cell-mediated immune responses.
abstract: Hartley guinea pigs injected subconjunctivally with Onchocerca lienalis (OL) microfilariae (Mf) develop punctate corneal opacities resembling the punctate keratitis of human onchocerciasis. Antibody production and antigen-induced proliferative responses were studied in conjunctival-associated lymphoid tissues (CALT), spleens (SL) and peripheral blood lymphocytes (PBL) from experimentally infected guinea pigs. Cultured single cell suspensions of CALT, SL and PBL were assayed for IgG1, IgG2, IgA and IgE antibody production. IgG1, IgG2, and IgA Onchocerca-specific antibodies were found in culture supernatants of CALT, SL and PBL. When initiated 10 days after a challenge injection of OL, CALT cultures produced antibody levels equal to or less than those produced by the corresponding SL cultures. When initiated 66 days after the last injection of Mf, CALT cultures produced significantly more antibody than the corresponding SL cultures. Blastogenic responses to OL Mf antigen were observed in peripheral and splenic lymphocytes of OL-infected guinea pigs. Animals given subconjunctival injections of Mf followed by treatment with a microfilaricide had greater responses to OL antigen than those given Mf alone, while responses to phytomitogens were similar in drug-treated and non-treated animals. The CALT was locally immunologically responsive against the subconjunctivally injected OL Mf, with the capacity for localized memory responses. The local immunologic responses to conjunctival Onchocerca microfilariae may play a significant role in the immunopathological reactions of ocular onchocerciasis.
authors: Department of Ophthalmology, Scheie Eye Institute, School of Medicine, University of Pennsylvania, Philadelphia.
authors: J P
authors: JP
authors: Haldar
authors: M
authors: M
authors: Khatami
authors: J B
authors: JB
authors: Lok
authors: J H
authors: JH
authors: Rockey
authors: J J
authors: JJ
authors: Donnelly
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis*; D003315:Cornea / Q000469:parasitology / Q000473:pathology; D004049:Diethylcarbamazine / Q000627:therapeutic use; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006168:Guinea Pigs; D007111:Immunity, Cellular; D007070:Immunoglobulin A / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D007150:Immunohistochemistry; D007559:Ivermectin / Q000627:therapeutic use; D008213:Lymphocyte Activation; D008221:Lymphoid Tissue / Q000276:immunology; D008842:Microfilariae / Q000276:immunology; D009854:Onchocerca / Q000276:immunology*; D015827:Onchocerciasis, Ocular / Q000188:drug therapy / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007559:Ivermectin; D004049:Diethylcarbamazine