Isolation and partial characterization of Bermuda grass-pollen allergen: BG-60b.
Our earlier studies have shown that the pollen of Bermuda grass (Cynodon dactylon)-pollen contain at least 12 IgE-binding proteins which can be analyzed by the immunoblot technique. One of the highly active components was found to be a basic protein with a molecular weight of 60,000 daltons, designated as BG-60. This component was showed to consist of a group of proteins. One of them, BG-60a (pI 9.7), has been isolated and characterized. In this study, we have further isolated and characterized the second component of the antigen, designated as BG-60b. Its purity was demonstrated by gel electrophoresis experiment and antigen-antibody precipitation studies. The antigen is of glycoprotein nature with a pI of 10.0. It exhibits IgE-binding activity and shows cross-reactivity to antigen BG-60a in double diffusion. Its chemical and physical properties are similar to antigen BG-60a.
Su
S N
SN
Dept. of Medical Research, Veterans General Hospital, Taipei, Taiwan, Republic of China.
Lau
G X
GX
Yang
S Y
SY
Shen
H D
HD
Tsai
J J
JJ
Han
S H
SH
D000485:Allergens / Q000276:immunology / Q000302:isolation & purification*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003429:Cross Reactions; D007073:Immunoglobulin E / Q000276:immunology; D010940:Plant Proteins / Q000276:immunology / Q000302:isolation & purification*; D006109:Poaceae / Q000276:immunology*; D011058:Pollen / Q000276:immunology*
D000485:Allergens; D010940:Plant Proteins; D007073:Immunoglobulin E