Characterization of human autoantibodies specific for lamin A.
We have characterized human autoimmune polyclonal antibodies reactive with lamin A, a 74 kDa peripheral protein of the nuclear envelope. Unlike other known antibodies to lamin A, the antibodies described here do not crossreact with the structurally related lamin C. These antibodies feature only chi light chains suggesting that their specificity is restricted to a limited number of epitopes. Based on the known amino acid sequence of human lamins A and C, the epitope(s) are most likely located in the 80 amino acid carboxyl tail of mature lamin A.
Courvalin
J C
JC
Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, NY 10021.
Chaudhary
N
N
Danon
F
F
Brouet
J C
JC
Lassoued
K
K
D000918:Antibody Specificity; D001323:Autoantibodies / Q000097:blood*; D005455:Fluorescent Antibody Technique; D006801:Humans; D034904:Lamin Type A; D034882:Lamins; D008099:Liver / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000276:immunology*
D001323:Autoantibodies; D034904:Lamin Type A; D034882:Lamins; D009687:Nuclear Proteins; C440308:lamin C