Anaerobic degradation of acetone by Desulfococcus biacutus spec. nov.
From anaerobic digestor sludge of a waste water treatment plant, a gram-negative, strictly anaerobic sulfate-reducing bacterium was isolated with acetone as sole organic substrate. The bacterium was characterized as a new species, Desulfococcus biacutus. The strain grew with acetone with doubling times of 72 h to 120 h; the growth yield was 12.0 (+/- 2.1) g x [mol acetone]-1. Acetone was oxidized completely, and no isopropanol was formed. In labelling studies with 14CO2, cell lipids (including approx. 50% PHB) of acetone-grown cells became labelled 7 times as high as those of 3-hydroxy-butyrate-grown cells. Enzyme studies indicated that acetone was degraded via acetoacetyl-CoA, and that acetone was channeled into the intermediary metabolism after condensation with carbon dioxide to a C4-compound, possibly free acetoacetate. Acetoacetyl-CoA is cleaved by a thiolase reaction to acetyl-CoA which is completely oxidized through the carbon monoxide dehydrogenase pathway. Strain KMRActS was deposited with the Deutsche Sammlung von Mikroorganismen, Braunschweig, under the number DSM 5651.
Platen
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Mikrobiologie I, Eberhard-Karls-Universität, Tübingen, Federal Republic of Germany.
Temmes
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Schink
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B
D020155:3-Hydroxybutyric Acid; D000090:Acetoacetates / Q000378:metabolism; D000096:Acetone / Q000378:metabolism*; D001421:Bacteria, Anaerobic / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D003470:Culture Media; D005618:Fresh Water; D006885:Hydroxybutyrates / Q000378:metabolism; D012722:Sewage; D014865:Waste Disposal, Fluid; D014871:Water Microbiology
D000090:Acetoacetates; D003470:Culture Media; D006885:Hydroxybutyrates; D012722:Sewage; D000096:Acetone; C016635:acetoacetic acid; D020155:3-Hydroxybutyric Acid