The nucleotide sequence of the Desulfovibrio gigas desulforedoxin gene indicates that the Desulfovibrio vulgaris rbo gene originated from a gene fusion event.
Expression of the rbo gene from Desulfovibrio vulgaris Hildenborough in Escherichia coli minicells and Western blotting (immunoblotting) of Desulfovibrio cell extracts with antibodies raised against a synthetic peptide indicated the presence of a 14-kDa polypeptide product, as expected from the gene sequence. Cloning and sequencing of the gene (dsr) for desulforedoxin, a 4-kDa redox protein from Desulfovibrio gigas, showed that it is formed by expression of an autonomous gene of 111 bp, not by processing of a 14-kDa protein. The results indicate that the rbo gene product, which has a 4-kDa desulforedoxin domain as the NH2 terminus, may have arisen by gene fusion. Shuffling and fusion of genes for redox protein domains can explain the large variety of redox proteins found in sulfate-reducing bacteria.
Brumlik
M J
MJ
Department of Biological Sciences, University of Calgary, Alberta, Canada.
Leroy
G
G
Bruschi
M
M
Voordouw
G
G
D000595:Amino Acid Sequence; D001483:Base Sequence; D005075:Biological Evolution; D015153:Blotting, Western; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D003901:Desulfovibrio / Q000235:genetics* / Q000276:immunology; D005798:Genes, Bacterial; D007506:Iron-Sulfur Proteins / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009876:Operon; D012416:Rubredoxins / Q000378:metabolism; D013045:Species Specificity
D004269:DNA, Bacterial; D007506:Iron-Sulfur Proteins; D012416:Rubredoxins; C022220:desulforedoxin