New Secale cereale (rye) DNA derivatives for the detection of rye chromosome segments in wheat.
Subcloning of a clone of the 120-bp family of rye, pSc119, has produced two extremely useful probes. pSc119.1 assays rye-specific dispersed repetitive sequence families. It is present on all seven rye chromosomes and hybridizes to the entire length of each chromosome, with the exception of some telomeres and the nucleolar organiser region. pSc119.2, in contrast, hybridizes predominantly to the telomeric regions of rye chromosomes, with some interstitial sites. Unlike pSc119.1, it assays similar repetitive sequence families in both wheat and rye chromosomes.
McIntyre
C L
CL
Department of Agronomy, University of Missouri, Columbia 65211.
Pereira
S
S
Moran
L B
LB
Appels
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R
D002875:Chromosomes; D002999:Clone Cells; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D007621:Karyotyping; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D012434:Secale / Q000235:genetics*; D014908:Triticum / Q000235:genetics*
D015342:DNA Probes; D004247:DNA