In vivo flow 31P FT-NMR spectroscopy of a nematode parasite.
31P NMR spectroscopy of a parasitic nematode. Steinernema carpocapsae, is described. In vivo spectra were generated from nematode suspensions continuously circulating through the spectrometer. By flowing the organism, saturation effects were avoided and short interpulse delays significantly reduced spectral generation time. To maximize sensitivity, 90 degrees pulses were employed. Changes in energetic status in response to oxygen availability were easily and rapidly monitored in circulating nematodes.
Thompson
S N
SN
Analytical Chemistry Instrumentation Facility, University of California, Riverside 92521.
Platzer
E G
EG
Lee
R W
RW
D000818:Animals; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009348:Nematoda / Q000033:anatomy & histology*; D010758:Phosphorus
D010758:Phosphorus