Shark tooth morphogenesis. An SEM and EDX analysis of enameloid and dentin development in various shark species.
The study provides a survey of shark tooth morphogenesis based on SEM and EDX analyses of whole tooth families in six shark species. The teeth, demonstrating different stages of development, were acid-etched and coated with palladium. Calcium content was determined semi-quantitatively by using the palladium coating as an internal standard. Due to the rapid development of the enameloid, all major events took place in the two or three youngest teeth of a tooth family. Enameloid appeared to develop as a transformation of the peripheral part of the dental papilla. Mineralization started immediately. Based on morphological criteria the middle zone of the enameloid was established at an early stage, excluding the possibility of an unambiguous centrifugal or centripetal direction of growth. Substantial mineral increase first occurred in the middle zone, spreading from the tooth tip toward the base. Dentin formed after the enameloid was completely established. Dentin formation started basally as a direct prolongation of the enameloid cap, then spreading toward the tooth tip, first along the edges. It is concluded that shark enameloid has a mesenchymal background, but a role played by the inner dental epithelium can not be excluded.
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Department of Anatomy, Dental Faculty, University of Oslo, Norway.
D000566:Amelogenesis / Q000502:physiology*; D000818:Animals; D002118:Calcium / Q000032:analysis; D003743:Dental Enamel / Q000737:chemistry / Q000648:ultrastructure*; D003771:Dental Papilla / Q000648:ultrastructure; D003782:Dental Pulp / Q000648:ultrastructure; D003804:Dentin / Q000737:chemistry / Q000648:ultrastructure*; D003810:Dentinogenesis / Q000502:physiology*; D004577:Electron Probe Microanalysis; D008855:Microscopy, Electron, Scanning; D009024:Morphogenesis / Q000502:physiology; D009805:Odontogenesis / Q000502:physiology*; D012754:Sharks / Q000033:anatomy & histology / Q000378:metabolism / Q000502:physiology*
D002118:Calcium