A differential lysis method for the isolation of Cowdria ruminantium DNA.
In order to isolate pure Cowdria ruminantium DNA an enzymatic lysis procedure was used to lyse Cowdria-infected bovine endothelial cell cultures differentially. Infected host cells were treated with trypsin followed by DNase digestion and centrifugation. This method resulted in the isolation of intact Cowdria organisms and removed bovine DNA effectively.
Wilkins
S C
SC
Molecular Biology Section, Veterinary Research Institute, Onderstepoort, South Africa.
Ambrosio
R E
RE
D000818:Animals; D015139:Blotting, Southern; D002417:Cattle; D002460:Cell Line; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D004727:Endothelium / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D012287:Rickettsiaceae / Q000235:genetics*; D014357:Trypsin / Q000378:metabolism*
D015342:DNA Probes; D004269:DNA, Bacterial; D014357:Trypsin