Bidirectional promoter activity of the 5' flanking region of the mouse thymidine kinase gene.
The 5' flanking region of the gene coding for cytoplasmic thymidine kinase (TK) in the mouse (a total of 490 bp upstream of the initiation codon) was tested for promoter activity using the chloramphenicol acetyltransferase gene as reporter. It was found that the region can be divided into two parts, one of which carries promoter activity in the direction of TK, whereas the 5'-half has promoter activity in the opposite direction. A fragment of 140 bp was sufficient for growth-dependent promoter activity in the direction of TK, although about 100 bp further upstream, enhanced the activity. Expression from the divergent promoter was independent of cell growth.
Weichselbraun
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Institut für Molekularbiologie, Universität Wien, Austria.
Ogris
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Wintersberger
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D000818:Animals; D002455:Cell Division; D002460:Cell Line; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D066298:In Vitro Techniques; D051379:Mice; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D013937:Thymidine Kinase / Q000235:genetics*; D014158:Transcription, Genetic
D013937:Thymidine Kinase