Analysis of human vaginal secretions using rabbit anti-human vaginal secretions: detection of non-serum proteins.
The antigens detectable in human vaginal secretions (HVS) have been analyzed by using a rabbit anti-HVS serum. After absorption by a pool of human sera, this antiserum on immunoelectrophoretic analysis produced three precipitation lines between the HVS and the antiserum. These lines were found at gamma (a line), beta-gamma (b line) and beta (c line) globulin regions. Their respective incidences of appearance among 59 HVS specimens obtained from 50 women were 46 (78%), 56 (95%) and 29 (49%). Of these HVS specimens, 57 (97%) were positive for one or more of these antigens. These three antigens, a, b, and c can be considered useful markers to determine the presence of the HVS. The origin of the HVS proteins and the influence of the non-serum proteins from the upper genital tracts are also discussed.
Itoh
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Department of Forensic Medicine, Juntendo University School of Medicine, Tokyo, Japan.
D000293:Adolescent; D000328:Adult; D000818:Animals; D000941:Antigens / Q000032:analysis; D005260:Female; D006801:Humans; D007044:Hysterectomy; D007106:Immune Sera; D007122:Immunoelectrophoresis; D008875:Middle Aged; D049590:Postpartum Period / Q000378:metabolism; D011247:Pregnancy / Q000378:metabolism; D011506:Proteins / Q000032:analysis* / Q000276:immunology; D011817:Rabbits; D014621:Vagina / Q000737:chemistry* / Q000276:immunology
D000941:Antigens; D007106:Immune Sera; D011506:Proteins