A rapid, sensitive assay for gamma-aminobutyric acid in brain using electron-capture gas chromatography.
A rapid, sensitive procedure has been developed for the assay of gamma-aminobutyric acid (GABA) in brain tissue. The method involves sequential reaction with isobutyl chloroformate and pentafluorophenol under aqueous conditions and subsequent separation and quantitation of the derivative on a gas chromatograph equipped with a capillary column and an electron-capture detector. The method produces a derivative with good stability and provides for simultaneous analysis of GABA and five other aliphaticamino acids in very small volumes of supernatant from brain homogenates.
Wong
J T
JT
PMHAC Research Unit, University of Alberta, Edmonton, Canada.
Baker
G B
GB
Coutts
R T
RT
D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D001923:Brain Chemistry; D002849:Chromatography, Gas / Q000379:methods*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051381:Rats; D011919:Rats, Inbred Strains; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
D000596:Amino Acids; D005680:gamma-Aminobutyric Acid