title: Crystallization of the Fab from a human monoclonal antibody against gp 41 of human immunodeficiency virus type I.
abstract: A monoclonal IgG antibody directed against gp 41 from the human immunodeficiency virus (HIV-1) has been crystallized in both intact and Fab forms. Crystals of the intact antibody grow as tetragonal-like prisms too small for conventional X-ray analysis. However, the Fab portion of the antibody produces suitable plate-like crystals which belong to the space group P2(1)2(1)2(1) with unit cell constants of a = 66.5 A, b = 74.3 A and c = 105.3 A. There is one molecule of Fab in the asymmetric unit. The Fab crystals show diffraction to d-spacings less than 3.0 A.
authors: Space Science Laboratory, Marshall Space Flight Center, Huntsville, AL 35812.
authors: E
authors: E
authors: Casale
authors: E
authors: E
authors: Wenisch
authors: X M
authors: XM
authors: He
authors: P G
authors: PG
authors: Righetti
authors: R S
authors: RS
authors: Snyder
authors: A
authors: A
authors: Jungbauer
authors: C
authors: C
authors: Tauer
authors: F
authors: F
authors: Ruker
authors: D C
authors: DC
authors: Carter
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry; D000937:Antigen-Antibody Reactions; D003460:Crystallization; D015700:HIV Envelope Protein gp41 / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D000911:Antibodies, Monoclonal; D015700:HIV Envelope Protein gp41; D007140:Immunoglobulin Fab Fragments