[Chemiluminescence measurements of bronchoalveolar lavage cells in patients with collagenosis].
The purpose of the study was to determine the role of reactive oxygen metabolites in the pathogenesis of interstitial lung involvement in 13 patients with collagen vascular diseases (CVD) in comparison with 10 control subjects. The cellular activity of bronchoalveolar lavage (BAL) cells was measured by means of chemiluminescence (CL), spontaneously and after challenge of the cells with opsonized zymosan (simultaneously luminol- and lucigenin-dependent). The findings show an increase of the spontaneous and stimulated CL of BAL cells in CVD, more pronounced in luminol-dependent CL. The luminol-dependent CL correlates with neutrophils in BAL cell differentiation. The results suggest that reactive oxygen metabolites take part in interstitial lung involvement in patients with CVD. Therefore the measurement CL of BAL cells is useful in addition to BAL cell differentiation in diagnostic of interstitial lung involvement in CVD.
Witt
C
C
Klinik für Innere Medizin Theodor Brugsch, Humboldt-Universität Berlin, DDR.
Winsel
K
K
Apostoloff
E
E
Brenke
A
A
Hiepe
F
F
Renner
H
H
D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D005609:Free Radicals; D006801:Humans; D007958:Leukocyte Count; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*; D010101:Oxygen Consumption / Q000276:immunology*; D011658:Pulmonary Fibrosis / Q000175:diagnosis / Q000276:immunology*; D012595:Scleroderma, Systemic / Q000175:diagnosis / Q000276:immunology*
D005609:Free Radicals
Chemilumineszenz-Messungen an Zellen der bronchoalveolären Lavage bei Kollagenose-Patienten.