title: K-252a inhibits the response of tomato cells to fungal elicitors in vivo and their microsomal protein kinase in vitro.
abstract: Two characteristic responses of plant cells to fungal elicitors, induction of phenylalanine ammonia-lyase activity and of ethylene biosynthesis, were studied in suspension-cultured tomato cells. Induction of both responses was completely blocked by 500 nM K-252a, a known inhibitor of mammalian protein kinases. About 100 nM K-252a caused half-maximal inhibition. In vitro, K-252a inhibited protein kinase activity in microsomal preparations from tomato cells. Inhibition was competitive with respect to ATP and had a Ki of about 15 nM. Thus, protein kinases sensitive to K-252a occur in plants and might be important for the plant's response to fungal elicitors.
authors: Friedrich Miescher-Institute, Basel, Switzerland.
authors: D G
authors: DG
authors: Grosskopf
authors: G
authors: G
authors: Felix
authors: T
authors: T
authors: Boller
mesh_terms: D002227:Carbazoles / Q000494:pharmacology*; D002473:Cell Wall; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005030:Ethylenes / Q000096:biosynthesis*; D005658:Fungi / Q000472:pathogenicity*; D005936:Glucans / Q000494:pharmacology; D006020:Glycopeptides / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000494:pharmacology; D026121:Indole Alkaloids; D010650:Phenylalanine Ammonia-Lyase / Q000096:biosynthesis*; D010940:Plant Proteins / Q000378:metabolism; D010944:Plants / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D047428:Protein Kinase Inhibitors; D043325:Xylan Endo-1,3-beta-Xylosidase
chemical_list: D002227:Carbazoles; D005030:Ethylenes; D005936:Glucans; D006020:Glycopeptides; D026121:Indole Alkaloids; D010940:Plant Proteins; D047428:Protein Kinase Inhibitors; C036216:ethylene; C049985:staurosporine aglycone; D006026:Glycoside Hydrolases; D043325:Xylan Endo-1,3-beta-Xylosidase; D010650:Phenylalanine Ammonia-Lyase