Lysosomal inhibitors stimulate resting NIH 3T3 cells to proliferate.
Lysosomal inhibitors (amino acid methyl esters) and platelet-derived growth factor stimulate resting NIH 3T3 cells to enter the S period. Incubation of cells in medium containing lysosomal inhibitors causes an increase in protein accumulation and does not disrupt lysosomes. The results indicate that proliferative homeostasis depends partially on the metabolic status of the cell and that catabolic processes activated in resting cells negatively influence prereplicative reactions.
Rosenwald
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IB
Laboratory for the Functional Morphology of Chromosomes, U.S.S.R. Academy of Sciences, Moscow.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004952:Esters / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006868:Hydrolysis; D008247:Lysosomes / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D051379:Mice; D008856:Microscopy, Fluorescence; D010982:Platelet-Derived Growth Factor / Q000032:analysis / Q000378:metabolism / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism; D016196:S Phase
D004952:Esters; D010982:Platelet-Derived Growth Factor; D011506:Proteins