title: Complete sequence of the extrachromosomal rDNA molecule from the ciliate Tetrahymena thermophila strain B1868VII.
abstract: The recent development of rDNA vectors for transformation of Tetrahymena combined with improved microinjection technology should lead to a renewed interest in this organism. In particular, the rDNA itself constitutes an attractive system for biochemical studies. The rDNA is amplified to a level of 2% of the total DNA and exists as extrachromosomal molecules. Furthermore, the rDNA is homogeneous in sequence because it is derived from a single gene during sexual reorganization. In order to facilitate studies of this molecule, we report here a compilation of previously published sequence information together with new sequence data that completes the entire sequence of the 21 kb rDNA molecule.
authors: Department of Biochemistry B, Panum Institute, University of Copenhagen, Denmark.
authors: J
authors: J
authors: Engberg
authors: H
authors: H
authors: Nielsen
mesh_terms: D000818:Animals; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000235:genetics*; D005111:Extrachromosomal Inheritance; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D013768:Tetrahymena / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D004275:DNA, Ribosomal