High-performance liquid chromatography of casein hydrolysates phosphorylated and dephosphorylated. I. Peptide mapping.
A mixture of small peptides of molecular weight averaging 1000 daltons, obtained by controlled hydrolysis of casein with proteases, chymotrypsin and trypsin, was separated by size-exclusion and reversed-phase high-performance liquid chromatography. Peptides were identified and located in the known casein structures from their amino acid content and their N- and C-terminal amino acid analyses. The primary structure of peptides identified from casein hydrolysate phosphorylated and casein hydrolysate dephosphorylated is presented.
Lemieux
L
L
Département de Sciences et Technologie des Aliments, Université Laval, Sainte-Foy, Québec, Canada.
Amiot
J
J
D000595:Amino Acid Sequence; D002364:Caseins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002918:Chymotrypsin / Q000378:metabolism; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D010449:Peptide Mapping; D010766:Phosphorylation; D014357:Trypsin / Q000378:metabolism
D002364:Caseins; D010446:Peptide Fragments; D002918:Chymotrypsin; D014357:Trypsin