[Enzyme indicator of the immunobiologic activity of antigens].
The test-system has been elaborated for the determination of the ability of different antigens in the cultures of the mouse peritoneal macrophages. Measuring an intracellular acid phosphatase activity, reflecting the extent of the cell activation, was taken as a principle of this test-system. With the help of this test-system ability of a few antigens (lipopolysaccharide, polysaccharide, glycoprotein, protein and low molecular weight substances--peptide and glycopeptide) have been studied. It is testified that above mentioned antigens possessed different effects on the acid phosphatase activity.
Drozhennikov
V A
VA
Beliakova
E A
EA
Medvedev
S A
SA
Krasil'nikov
I V
IV
D000135:Acid Phosphatase / Q000032:analysis*; D000818:Animals; D000941:Antigens / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology; D002478:Cells, Cultured; D004796:Clinical Enzyme Tests; D003470:Culture Media; D008264:Macrophages / Q000201:enzymology / Q000276:immunology*; D051379:Mice; D008808:Mice, Inbred CBA; D010537:Peritoneum; D011135:Polysaccharides, Bacterial
D000941:Antigens; D000942:Antigens, Bacterial; D003470:Culture Media; D011135:Polysaccharides, Bacterial; D000135:Acid Phosphatase
Fermentnyĭ pokazatel' immunobiologicheskoĭ aktivnosti antigenov.