Polymerase chain reaction for bcl-2 in diagnostic lymph node biopsies.
bcl-2 is a marker for the translocation t(14;18)(q32;q21) indicative of follicular B-cell lymphoma. We studied 115 cases of lymphoproliferative disease with the polymerase chain reaction for bcl-2 oncogene using biotin and radiolabeled probes to the major breakpoint and minor cluster regions. Twenty-three percent of B-cell lymphomas were positive for bcl-2. These included 12 of 20 cases of nodular follicular center cell lymphoma (nine small cleaved cell, one mixed small and large cell, and two large cell types). bcl-2 translocation was detected in only three of 45 cases of diffuse B-cell lymphoma, and cases of AIDS-related malignant lymphoma, monocytoid B-cell lymphoma, and mantle zone lymphoma were all negative. Nonneoplastic lymphoid proliferations were negative for bcl-2 including nine cases of abnormal follicular hyperplasia from patients with acquired immunodeficiency syndrome (AIDS) and AIDS-related complex. Cases of T-cell lymphoma and five cases of Hodgkin's disease were also negative. The polymerase chain reaction for bcl-2 is a rapid, sensitive technique in the evaluation of follicular B-cell proliferations, and the use of biotinylated probes and the alkaline phosphatase reaction eliminates the requirement for radioactive reagents.
Said
J W
JW
Department of Pathology and Laboratory Medicine, Cedars Sinai Medical Center, Los Angeles, California.
Sassoon
A F
AF
Shintaku
I P
IP
Corcoran
P
P
Nichols
S W
SW
D000386:AIDS-Related Complex / Q000235:genetics / Q000473:pathology; D000163:Acquired Immunodeficiency Syndrome / Q000235:genetics / Q000473:pathology; D001483:Base Sequence; D015139:Blotting, Southern; D002883:Chromosomes, Human, Pair 14; D002887:Chromosomes, Human, Pair 18; D004273:DNA, Neoplasm / Q000032:analysis; D006689:Hodgkin Disease / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D006801:Humans; D006965:Hyperplasia; D008198:Lymph Nodes / Q000473:pathology* / Q000648:ultrastructure; D008223:Lymphoma / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D016393:Lymphoma, B-Cell / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D014178:Translocation, Genetic
D004273:DNA, Neoplasm; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2