Receptor-mediated presynaptic facilitation of quantal release of acetylcholine induced by pralidoxime in Aplysia.
1. Possible interactions of contrathion (pralidoxime sulfomethylate), a reactivator of phosphorylated acetylcholinesterase (AChE), with the regulation of cholinergic transmission were investigated on an identified synapse in the buccal ganglion of Aplysia californica. 2. Transmitter release was evoked either by a presynaptic action potential or, under voltage clamp, by a long depolarization of the presynaptic cell. At concentrations higher than 10(-5) M, bath-applied contrathion decreased the amplitude of miniature postsynaptic currents and increased their decay time. At the same time, the quantal release of ACh was transiently facilitated. The facilitatory effect of contrathion was prevented by tubocurarine but not by atropine. Because in this preparation, these drugs block, respectively, the presynaptic nicotinic-like and muscarinic-like receptors involved in positive and negative feedback of ACh release, we proposed that contrathion activates presynaptic nicotinic-like receptors. 3. Differential desensitization of the presynaptic receptors is proposed to explain the transience of the facilitatory action of contrathion on ACh release. 4. The complexity of the synaptic action of contrathion raises the possibility that its therapeutic effects in AChE poisonings are not limited to AChE reactivation.
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Laboratoire de Neurobiologie Cellulaire et Moléculaire, CNRS, Gif sur Yvette, France.
Baux
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Poulain
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Tauc
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D000109:Acetylcholine / Q000378:metabolism*; D000110:Acetylcholinesterase / Q000378:metabolism; D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001048:Aplysia / Q000502:physiology; D001285:Atropine / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D006426:Hemicholinium 3 / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D010091:Oximes; D011220:Pralidoxime Compounds / Q000494:pharmacology*; D011726:Pyridinium Compounds / Q000494:pharmacology; D011978:Receptors, Nicotinic / Q000187:drug effects* / Q000502:physiology; D012636:Secretory Rate / Q000187:drug effects; D013569:Synapses / Q000187:drug effects*; D014403:Tubocurarine / Q000494:pharmacology
D010091:Oximes; D011220:Pralidoxime Compounds; D011726:Pyridinium Compounds; D011978:Receptors, Nicotinic; D006426:Hemicholinium 3; D001285:Atropine; D000110:Acetylcholinesterase; C022870:asoxime chloride; D000109:Acetylcholine; C028797:pralidoxime; D014403:Tubocurarine