Isolation and characterization of a galactose-specific carbohydrate binding protein from human lymphoblastic cells.
A carbohydrate binding protein of Mr = 32,000 (CBP 32) has been isolated from detergent extracts of human B and T lymphoblastoid cells. CBP 32 binds specifically to glycoproteins containing asparagine-linked complex oligosaccharides, and can be eluted from a fetuin affinity matrix by beta-lactose. Binding is not thiol dependent, nor are divalent cations necessary for binding. Native CBP 32 appears to exist as a monomer, with a pI of 8.2. Purified CBP 32 can bind detergent, as shown by charge-shift electrophoresis, and thus appears to be an integral membrane protein.
Baum
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Department of Pathology, UCLA School of Medicine 90024.
Cresswell
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D001402:B-Lymphocytes / Q000737:chemistry*; D002352:Carrier Proteins / Q000302:isolation & purification*; D002460:Cell Line; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D005697:Galactosides / Q000378:metabolism; D037161:Galectins; D006388:Hemagglutinins / Q000302:isolation & purification*; D006801:Humans; D007525:Isoelectric Focusing; D008970:Molecular Weight; D013601:T-Lymphocytes / Q000737:chemistry*
D002352:Carrier Proteins; D003902:Detergents; D005697:Galactosides; D037161:Galectins; D006388:Hemagglutinins