[The effect of ethanol and the catalase inhibitor 3-amino-1,2,4-triazole on lipid peroxidation in rat liver homogenate and subcellular fractions].
Effects of repeated administration of ethanol and the catalase inhibitor 3-amino-1,2,4-triazole on the rate of Fe2+/ADP-ascorbate induced (nonenzymatic) lipid peroxidation were studied in rat liver tissue homogenate and subcellular fractions using estimation of low-level chemiluminescence and malonic dialdehyde content. The rate of lipid peroxidation was decreased in whole and nuclear-free homogenates as a result of combined or individual ethanol and aminotriazole treatment. However, this pattern was unaltered in mitochondrial and microsomal fractions. Both these agents did not affect the content of conjugated dienes in lipid containing extracts of subcellular fractions as well as the total malonic dialdehyde concentration in whole liver tissue homogenate. The data obtained suggest that antioxidative protein factor, inhibiting nonenzymatic lipid peroxidation in biological membranes, was induced in liver cytosol of rats repeatedly administered with ethanol and/or amino-triazole.
Antonenkov
V D
VD
Pirozhkov
S V
SV
Popova
S V
SV
Panchenko
L F
LF
D000640:Amitrole / Q000494:pharmacology*; D000818:Animals; D002374:Catalase / Q000037:antagonists & inhibitors*; D000431:Ethanol / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000378:metabolism*
D000431:Ethanol; D002374:Catalase; D000640:Amitrole
Vliianie étanola i ingibitora katalazy 3-amino-1,2,4-triazola na perekisnoe okislenie lipidov v gomogenate i subkletochnykh fraktsiiakh pecheni krysy.