Attempt to show inhibition by oxygen in the modifying factor assay.
An Australian laboratory has demonstrated that antibodies to certain strains of enteric bacteria react with cells from HLA-B27-positive patients with a 'modifying factor' (MF) from these bacteria. These observations have proved difficult to reproduce. We investigated the possibility that oxygen inhibits the test by blocking B27, but we were unable to obtain consistent positive results.
Archer
J R
JR
Bone and Joint Research Unit, London Hospital Medical College, United Kingdom.
Claxson
A
A
Edwards
A
A
Thompson
P W
PW
D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D003429:Cross Reactions; D003470:Culture Media; D003601:Cytotoxicity Tests, Immunologic; D015796:HLA-B27 Antigen / Q000276:immunology*; D006801:Humans; D007709:Klebsiella / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D013167:Spondylitis, Ankylosing / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D003470:Culture Media; D015796:HLA-B27 Antigen; C051103:Klebsiella modifying factor; D010100:Oxygen