Mapping of the region of the tick-borne encephalitis virus replicase adjacent to initiating substrate binding center.
Affinity labelling with aldehyde-containing analogs of initiation substrates of nuclear fraction of tick-borne encephalitis virus (TBEV) infected cells results in a labelling of a single polypeptide with a molecular mass of 68 kDa which was immunologically identified as TBEV NS3 protein. A single-hit hydroxylamine hydrolysis, using limited and long-term CNBr cleavages allowed one to identify Lys1800 and/or Lys1803 as the label attachment sites. These amino acid residues are situated in the proximity of the 'B'-site of NTP-binding motif of viral RNA replicase.
Morozova
O V
OV
Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of the Academy of Sciences of the USSR.
Mustaev
A A
AA
Belyavskaya
N A
NA
Zaychikov
E F
EF
Kvetkova
E A
EA
Wolf YuI
Pletnev
A G
AG
D000255:Adenosine Triphosphate / Q000378:metabolism; D000345:Affinity Labels; D001665:Binding Sites; D003488:Cyanogen Bromide / Q000737:chemistry; D004669:Encephalitis Viruses, Tick-Borne / Q000201:enzymology*; D019811:Hydroxylamine; D006898:Hydroxylamines / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry; D010449:Peptide Mapping; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry* / Q000378:metabolism
D000345:Affinity Labels; D006898:Hydroxylamines; D010446:Peptide Fragments; D019811:Hydroxylamine; D000255:Adenosine Triphosphate; D012324:RNA-Dependent RNA Polymerase; D003488:Cyanogen Bromide