[In-vitro rates of fertilization and culture to blastocyst stage of mouse oocytes following co-incubation of murine spermatozoa with Escherichia coli and Ureaplasma urealyticum].
Mouse-spermatozoa were released of the ductus deferens and coincubated with different strains of E. coli resp. U. urealyticum before inseminated with oocytes of superovulated female mice. Daily light microscopic observations of embryonic development took place. Coincubation of spermatozoa with bacteria concentration of 10(3) cfu/ml E. coli revealed no changes in fertilization or culture rates as compared to the uninfected control group. In the same way a coincubation with 10(6) cfu/ml had no influence on the fertilization rates in vitro, however the culture rates were considerably reduced. Only one strain of ureaplasma urealyticum effected after coincubation with spermatozoa a reduced fertilization rate, the embryonic development into blastocysts after in vitro fertilization of spermatozoa infected with ureaplasma urealyticum showed a marked reduction in all but two strains.
Grillo
M
M
Abteilung Frauenheilkunde und Geburtshilfe, Christian-Albrechts-Universität zu Kiel.
Meyer-Jungcurt
J
J
Ozdemir
S
S
Krause
R
R
Riedel
H H
HH
D000818:Animals; D003470:Culture Media; D004926:Escherichia coli / Q000254:growth & development*; D005260:Female; D005307:Fertilization in Vitro; D008297:Male; D051379:Mice; D009865:Oocytes / Q000254:growth & development*; D013094:Spermatozoa / Q000254:growth & development*; D013480:Superovulation; D014509:Ureaplasma / Q000254:growth & development*
D003470:Culture Media
Fertilisations- und Kulturraten zum Blastozystenstadium von Mausoozyten In-vitro nach Koinkubation von Mausspermatozoen mit Escherichia coli sowie Ureaplasma urealyticum.