Glycoprotein II from adrenal chromaffin granules is also present in kidney lysosomes.
Glycoprotein II (GP II) is a protein found in the membranes of chromaffin granules from adrenal medulla. Immunoblotting (one- and two-dimensional) revealed that this antigen is also present in liver and in kidney. Subcellular fractionation of the latter organ indicated that GP II was present in lysosomes. This was confirmed by immunoelectron microscopy. The antiserum against GP II immunolabelled the membranes of organelles which could be identified as lysosomes by the labelling of their contents with an antiserum against cathepsin D. Thus GP II is an antigen common to secretory vesicles and lysosomes.
Weiler
R
R
Department of Pharmacology, University of Innsbruck, Austria.
Steiner
H J
HJ
Schmid
K W
KW
Obendorf
D
D
Winkler
H
H
D000313:Adrenal Medulla / Q000737:chemistry / Q000648:ultrastructure*; D000818:Animals; D002417:Cattle; D002458:Cell Fractionation; D002837:Chromaffin Granules / Q000737:chemistry / Q000648:ultrastructure*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D039421:Integrin alpha2; D007672:Kidney Cortex / Q000737:chemistry / Q000648:ultrastructure*; D008247:Lysosomes / Q000737:chemistry / Q000648:ultrastructure*; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000302:isolation & purification; D016253:Microscopy, Immunoelectron; D008970:Molecular Weight
C056564:ITGA2B protein, human; D039421:Integrin alpha2; D008562:Membrane Glycoproteins