The use of tetramethylbenzidine for solid phase immunoassays.
A rapid, sensitive, solid-phase immunoassay, using horseradish peroxidase and 3,3',5,5'-tetramethylbenzidine, was found to be more sensitive and the colour developed was more stable compared with HRPO using 4-chloro-1-naphthol or diaminobenzidine. Assay sensitivity was equal to or greater than that obtained with the alkaline phosphatase reaction. The essential step was to incubate filters in 1% dextran sulphate for 10 min in a pH 5 (10 mM citrate-EDTA) buffer before reacting with TMB (0.05 mg/ml in the same buffer).
McKimm-Breschkin
J L
JL
CSIRO Division of Biomolecular Engineering, Parkville, Australia.
D001560:Benzidines; D003116:Color; D006735:Horseradish Peroxidase; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D012680:Sensitivity and Specificity
D001560:Benzidines; C021758:3,3',5,5'-tetramethylbenzidine; D006735:Horseradish Peroxidase