Effects of a first exposure to ethanol on the compositions of neutral and polar lipids in Euglena gracilis Z, taken as a hepatic cell model: equilibration by citrulline-malate.
In comparison to the lipid composition of Euglena cells fed with lactate, a first exposure of the cells to ethanol favors the production of neutral lipids containing mainly unsaturated fatty acids. The ethanol diminishes drastically the proportion of PC and weakly that of PE. In contrast, it increases slightly the proportion of DPG. The ethanol induces important changes in the fatty acid distributions of each lipid class, suggesting modifications of the elongation-desaturation system. On the one hand the proportion of unsaturated fatty acids is increased and, on the other hand, the last double bond is predominantly situated in the delta 6 position in place of delta 3. The addition of the complex citrulline-malate corrects most of these changes.
Thuillier-Bruston
F
F
Laboratoire Biocodex, Montrouge, France.
Briand
J
J
Laval-Martin
D
D
D000818:Animals; D002478:Cells, Cultured; D002956:Citrulline / Q000494:pharmacology*; D000431:Ethanol / Q000494:pharmacology*; D005055:Euglena / Q000187:drug effects* / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis; D008055:Lipids / Q000032:analysis*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008293:Malates / Q000494:pharmacology*; D008954:Models, Biological
D005227:Fatty Acids; D008055:Lipids; D008293:Malates; D002956:Citrulline; D000431:Ethanol; C030298:malic acid