Synthesis and enzymatic polymerisation of 5-amino-1-(2'-deoxy-beta-D-ribofuranosyl)imidazole-4-carboxamide-5'- triphosphate.
The chemical synthesis of 5-amino-1-(2'-deoxy-beta-D-ribofuranosyl)imidazole-4-carboxamide, referred to as dZ, and of its 5'-triphosphate derivative (dZTP), from 2'-deoxyinosine is described. The polymerisation of dZTP using terminal deoxynucleotidyltransferase to give a homopolymer is also presented.
Pochet
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Institut Pasteur, Unité de Chimie Organique, URA-CNRS 487, Paris, France.
D'Ari
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D000620:Aminoimidazole Carboxamide / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D003853:Deoxyribonucleosides / Q000138:chemical synthesis; D003854:Deoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D011108:Polymers / Q000737:chemistry / Q000378:metabolism
D003853:Deoxyribonucleosides; D003854:Deoxyribonucleotides; D011108:Polymers; C066661:5-amino-1-(2'-deoxy-beta-ribofuranosyl)imidazole-4-carboxamide-5'-triphosphate; D000620:Aminoimidazole Carboxamide; C066662:5-amino-1-(2'-deoxy-beta-D-ribofuranosyl)imidazole-4-carboxamide