[Use of cytochalasin B in the micronucleus test in human peripheral lymphocytes].
The micronucleus test is one of the alternative procedures of cytogenetic analysis. Its modification with the use of cytochalasine B (Calbiochem AG) ensures safely the recording of changes of the genetic apparatus in the first cellular cycle after an attack of a mutagenic agent on human peripheral lymphocytes. The authors elaborated a reproducible modification of the original work of Fenech and Morley--published in this country by Kocisová and Srám--and describe in detail its individual steps. The cytochalasine MN-test which should become part of obligatory standard procedures of Czechoslovak preventive health services (formerly hygiene service) is thus open to confirmation.
Kubzová
E
E
Oddĕlení genetické toxikologie, Krajské hygienické stanice, Hradec Králové.
Vanícková
H
H
Ondrácková
M
M
Srb
V
V
D003571:Cytochalasin B; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure; D015162:Micronucleus Tests / Q000379:methods*
D003571:Cytochalasin B
Aplikace cytochalasin B mikronukleus testu v lidských periferních lymfocytech.