Sequence analysis and HLA-DR genotyping of a novel HLA-DRw14 allele.
Analysis of a Japanese population by oligonucleotide genotyping revealed that one Japanese HLA-DRw14 allele had a DRB1 genotype different from that of the known HLA-DRw14-related alleles, DRB1*1401 (DRw14-Dw9) and DRB1*1402 (DRw14-Dw16). The second exon of the DRB1 gene of the novel DRw14 allele (designated DRB1-14c) was amplified enzymatically and sequenced after cloning into a plasmid vector. The amino acid sequence of the first domain in the DR beta 1 chain encoded in the DRB1-14c allele was more similar to that of the DRB1*1401 allele (three amino acid substitutions) than to that of the DRB1*1402 allele (six amino acid substitutions). No polymorphic amino acid residue that could explain the common serologic HLA-DRw14 specificity was identified among the sequences of the three DRw14-related alleles. Sequence-specific oligonucleotides (SSOs) were synthesized on the basis of the DRB1-14c nucleotide sequence and used for genotyping of the Japanese population. These SSOs served as useful probes for identifying the DRB1-14c allele in a wide range of donors.
Obata
F
F
Department of Transplantation Immunology, Kitasato University School of Medicine, Sagamihara, Japan.
Ito
I
I
Ito
K
K
Abe
A
A
Ohkubo
M
M
Onda
K
K
Yang
Y G
YG
Watanabe
K
K
Kashiwagi
N
N
D000483:Alleles; D000595:Amino Acid Sequence; D001402:B-Lymphocytes / Q000276:immunology; D001483:Base Sequence; D015342:DNA Probes; D004591:Electrophoresis, Polyacrylamide Gel; D005838:Genotype; D006684:HLA-DR Antigens / Q000235:genetics*; D059810:HLA-DR Serological Subtypes; D006801:Humans; D007564:Japan; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D006684:HLA-DR Antigens; D059810:HLA-DR Serological Subtypes; C052238:HLA-DR14