The 60 and 63 kDa proteolytic peptides of the red cell membrane band-3 protein: their prevalence in human and non-human primates.
Three phenotypes based on the polymorphism of band-3 protein from human red cells are described. Limited proteolysis of intact red cells from most individuals (homozygotes) yields a peptide of 60 kDa, but in some cases (heterozygotes), there is also a 63-kDa peptide, and rarely only the single peptide of 63 kDa is found. This is the first description of the 63-kDa homozygote. The interpretation that the three phenotypes are controlled by two alleles of a single autosomal locus, with no dominance, is supported by population and family studies. The frequencies of the allele, which we designate as p63, is 0.041 +/- 0.0068 in Caucasoids and 0.125 +/- 0.0121 in Negroids. The electrophoretic profiles and molecular weights of the peptides obtained with several commercial proteases from Streptomyces griseus are similar to those obtained with chymotrypsin. Whereas band-3 protein in two New-World monkeys (Saimiri and Cebus) resisted pronase attack, an Old-World monkey (Macaca mulatta) was monomorphic for a 63-kDa fragment, and in an ape (Pan troglodytes), a doublet of 62 kDa and 64 kDa was found. Band-3 protein polymorphism appears to be a good marker for genetic differentiation in human populations.
Palatnik
M
M
Serviço de Hemoterapia, Hospital Universitário Clementino Fraga Filho, Rio de Janeiro, Brazil.
Simões
M L
ML
Alves
Z M
ZM
Laranjeira
N S
NS
D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte / Q000235:genetics*; D005075:Biological Evolution; D010450:Endopeptidases; D004910:Erythrocyte Membrane / Q000737:chemistry; D015186:Hominidae / Q000097:blood / Q000235:genetics*; D006801:Humans; D006868:Hydrolysis; D008970:Molecular Weight; D010455:Peptides / Q000097:blood* / Q000235:genetics; D011323:Primates / Q000097:blood / Q000235:genetics*
D001457:Anion Exchange Protein 1, Erythrocyte; D010455:Peptides; D010450:Endopeptidases