Effect of breast cyst fluid on oestrogen 17-oxidoreductase activity in MCF-7 breast cancer cells.
Breast cyst fluid (BCF) was found to stimulate oestrogen 17-oxidoreductase activity in the reductive direction, i.e., conversion of oestrone (E1) to oestradiol (E2), in MCF-7 breast cancer cells. Dialysis of BCF revealed that this property of BCF was present in both dialysed BCF and dialysate, implying that both high and low mol. wt. substances were responsible for stimulating E1 to E2 conversion. Gel filtration of dialysed BCF revealed that the high mol. wt. substances responsible for the stimulation of E1 to E2 conversion had mol. wts. of approximately 11 kD and 68 kD. This property of BCF would serve to increase the concentration of E2, a steroid which may play a role in mammary carcinogenesis.
Lai
L C
LC
Department of Chemical Pathology, St. Mary's Hospital Medical School, London, U.K.
Coldham
N G
NG
Islam
S
S
Reed
M J
MJ
Ghilchik
M W
MW
James
V H
VH
D001826:Body Fluids / Q000502:physiology; D001943:Breast Neoplasms / Q000201:enzymology* / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D002850:Chromatography, Gel / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003956:Dialysis; D004958:Estradiol / Q000378:metabolism; D004960:Estradiol Dehydrogenases / Q000378:metabolism*; D004967:Estrogens / Q000378:metabolism; D004970:Estrone / Q000378:metabolism; D005260:Female; D005348:Fibrocystic Breast Disease / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D014407:Tumor Cells, Cultured
D004967:Estrogens; D004970:Estrone; D004958:Estradiol; D004960:Estradiol Dehydrogenases