[Enzyme-linked immunosorbent assay of larvicidal toxic proteins of Bacillus sphaericus Ts-1].
Bacillus sphaericus Ts-1 Mosquito larvicidal toxins 42 k Da and 43 k Da were isolated by Sephadex G-200 chromatography. Three strains of highly toxic B. sphaericus and two non toxic strains were screened for toxic proteins using ELISA. The lowest detectable toxin level was 1.56 X 10(-5) mg/ml. Non toxic strains did not produce antigens reacting to either the 42 kDa or the 43 kDa antibodies. Ts-1 cultures were examined at 12 and 24 h by LC50 bioassay against Culex pipiens. The LC50's at 12 h and 24 h were 0.71 ppm and 0.154 ppm, respectively, i.e., the toxin level at 24 h was 4.6 times the level at 12 h. ELISA tests established total toxin at 0.049 mg/ml and 0.225 mg/ml at 12 h and 24 h, respectively, confirming the LC50 study.
Wang
J
J
Department of Biology, Nankai University, Tianjin.
Ren
G
G
Shang
K
K
D001407:Bacillus / Q000032:analysis*; D001427:Bacterial Toxins / Q000032:analysis* / Q000302:isolation & purification; D001681:Biological Assay; D004797:Enzyme-Linked Immunosorbent Assay
D001427:Bacterial Toxins; C046134:binB protein, Bacillus sphaericus