title: Production and characteristics of the exocellular polysaccharide of a mutant Mycobacterium strain.
abstract: Mutant strains of Mycobacterium sp. V-649 producing highly mucous colonies on a solid cultivation medium were prepared after treatment with N-methyl-N'-nitro-N-nitrosoguanidine and production of the exocellular polysaccharide was tested. The strains were cultivated in media with suitable sugar sources under submerged conditions. It was found that Mycobacterium sp. V-649/15 produces a maximum of 15-19% polymer after a 5-6-d cultivation. Gas chromatography indicated that the exocellular polysaccharide produced by this strain is of glucan type.
authors: Institute of Microbiology, Czechoslovak Academy of Sciences, Prague.
authors: J
authors: J
authors: Konícek
authors: G Y
authors: GY
authors: Daraselia
authors: M
authors: M
authors: Konícková-Radochová
authors: J
authors: J
authors: Lasík
authors: M
authors: M
authors: Wurst
authors: T
authors: T
authors: Koza
mesh_terms: D002849:Chromatography, Gas; D003470:Culture Media; D009154:Mutation; D009161:Mycobacterium / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000737:chemistry
chemical_list: D003470:Culture Media; D011135:Polysaccharides, Bacterial