Investigation of the repair of single-strand breaks in human DNA using alkaline gel electrophoresis.
Unstimulated lymphocytes from eight healthy persons were exposed to 10-, 30-, and 100-Gy doses of 60Co gamma radiation. The repair of damaged DNA was measured by (1) alkaline gel electrophoresis (extracted DNA loaded on 0.25% agarose gel, run at 1 V/cm for 39-44 h) at 0, 1, and 2 h after exposure and (2) incorporation of [3H]thymidine into unstimulated lymphocytes in the presence of 2 mM hydroxyurea 1 and 2 h after exposure. Both methods--alkaline gel electrophoresis and thymidine incorporation--showed that repair was completed within 2 h.
Kovacs
E
E
Department of Research, University Clinics, Kantonsspital, Basel, Switzerland.
Langemann
H
H
D003037:Cobalt Radioisotopes; D004249:DNA Damage; D004260:DNA Repair; D004277:DNA, Single-Stranded / Q000528:radiation effects*; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D066298:In Vitro Techniques
D003037:Cobalt Radioisotopes; D004277:DNA, Single-Stranded