title: Experimental and theoretical dynamics of isoelectric focusing: IV. Cathodic, anodic and symmetrical drifts of the pH gradient.
abstract: The production of anodic, cathodic and symmetrical drifts of a pH 3.5-10 gradient formed by isoelectric focusing in polyacrylamide gels is demonstrated experimentally by manipulation of the electrolyte concentrations. Experimental behavior is reproduced by computer simulation of a model mixture of 15 hypothetical carrier ampholytes whose pIs span the pH range 3-10. The mechanism which produces the drifts is elucidated and approaches to minimize such drifts are discussed. The data suggest why most experimentally observed drifts are cathodic.
authors: Center for Separation Science, University of Arizona, Tucson 85721.
authors: R A
authors: RA
authors: Mosher
authors: W
authors: W
authors: Thormann
mesh_terms: D003198:Computer Simulation; D004566:Electrodes; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D010756:Phosphoric Acids; D015203:Reproducibility of Results; D012972:Sodium Hydroxide
chemical_list: D010756:Phosphoric Acids; D012972:Sodium Hydroxide; C030242:phosphoric acid