Spontaneously metastasizing variants of a human lung carcinoma cell line: monoclonal antibody characterization.
Spontaneously occurring microscopic lung and lymph node metastases (in athymic mice) of a low metastatic human lung carcinoma cell line, UCP3, and its high metastatic variant, MV522, were isolated. Characterization of the variants included karyotypic and isoenzyme analyses; assessment of spontaneous metastatic capabilities in athymic mice, and monoclonal antibody analyses. The high metastatic variant LNT had barely detectable amounts of a glycoprotein molecule with apparent Mr 73 kd and 90 kd, which was present in the other cell lines. This molecule was detected in 20/24 primary human neoplasms but only in 3/18 metastatic neoplasms, suggesting a loss during the metastatic disease process.
Varki
N M
NM
Department of Medicine, University of California, San Diego, La Jolla.
Estes
L A
LA
Tseng
A
A
Vu
T P
TP
D000818:Animals; D000911:Antibodies, Monoclonal; D002455:Cell Division; D002460:Cell Line; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D007621:Karyotyping; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D016296:Mutagenesis; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation
D000911:Antibodies, Monoclonal; D006023:Glycoproteins