Trial and error in producing ankylosing-spondylitis-selective antisera according to Andrew Geczy.
Geczy found that rabbit sera raised against Klebsiella strain K43 cross-reacted with the cells from HLA-B27 positive patients with ankylosing spondylitis (AS). Other laboratories failed to reproduce these results. After a series of unsuccessful attempts, however, we managed to prepare one selective antiserum, using E. coli, isolated from a Dutch Bechterew patient, in offspring of rabbits Geczy sent us. Ever since we obtained irreproducible results only. This paper reports about the many attempts we have made to produce a discriminating antiserum for use in a combined vital stain and dye-exclusion assay.
Beukelman
C J
CJ
Eijkman-Winkler Laboratory of Medical Microbiology, Academic Hospital, Utrecht, The Netherlands.
Quarles van Ufford
H C
HC
van Bree
F P
FP
Aerts
P C
PC
Nieuwenhoff
C
C
Reerink
G
G
van Leeuwen
A
A
van Dijk
H
H
D000818:Animals; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic; D004926:Escherichia coli / Q000276:immunology; D005260:Female; D015796:HLA-B27 Antigen / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000096:biosynthesis* / Q000276:immunology; D007709:Klebsiella / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D015203:Reproducibility of Results; D013167:Spondylitis, Ankylosing / Q000276:immunology*
D015796:HLA-B27 Antigen; D007106:Immune Sera