Simultaneous synthesis of multiple oligonucleotides using nucleoside-H-phosphonate intermediates.
A semimechanized simultaneous synthesis of multiple oligonucleotides using nucleoside-H-phosphonates as intermediates was developed and applied to the preparation of hybridization probes. The synthesis could be done with equivalent results (about 98% average yield per cycle) using regenerated H-phosphonates.
Seliger
H
H
Universität Ulm, Sektion Polymere, Oberer Eselsberg, FRG.
Rösch
R
R
D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D005821:Genetic Techniques; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000138:chemical synthesis; D009841:Oligonucleotides / Q000138:chemical synthesis*; D063065:Organophosphonates / Q000737:chemistry
D015345:Oligonucleotide Probes; D009841:Oligonucleotides; D063065:Organophosphonates