Alterations in rabbit vitreal fine structure following C3F8 injection.
This study examines the morphological and histochemical changes in the cortical vitreous of 36 rabbit eyes following C3F8 intravitreal gas injection. Eyes were examined by light microscopy (LM) using a modified cryofixation and cryosectioning technique that prevented the loss of soluble tissue moieties and permitted collagen and proteoglycan histochemistry as well as enzyme digestion with hyaluronidase. LM and scanning electron microscopy (SEM) of cryosectioned normal eyes revealed an elaborate fibrillar matrix extending 100-190 microns from the basal lamina of the retina into the vitreous proper, which seemed to be composed of collagen fibrils intimately associated or wrapped in proteoglycan. Following the full expansion of the C3F8 gas bubble in the vitreous, the cortical fibrillar meshwork was absent from the retinal surface and a dense, collagenous material accumulated in the anterior vitreous, especially between the ciliary processes and over the posterior face of the lens. At 41 days postinjection, the fibrillar matrix was reforming and the vitreal cavity was filled with fluid and numerous fibrillar-mucinous islands. These islands did not form sheets or membranes, nor did they attach to either the posterior or the anterior retinal surface. The cortical fibrillar meshwork had reformed at 61 days' recovery; however, the condensed fibrillar material against the lens and filling the spaces between the ciliary processes had not resorbed. Neither shearing of the cortical gel or fibrillar matrix nor congestion of the anterior vitreous was observed in eyes only partially filled with gas.
Panessa-Warren
B
B
Department of Allied Health Resources, State University of New York, Stony Brook 11974.
Maisel
J M
JM
Warren
J
J
D000818:Animals; D003094:Collagen / Q000648:ultrastructure; D015925:Cryopreservation / Q000379:methods; D005109:Extracellular Matrix / Q000648:ultrastructure; D005466:Fluorocarbons / Q000494:pharmacology*; D006651:Histocytochemistry; D006821:Hyaluronoglucosaminidase; D008855:Microscopy, Electron, Scanning; D011509:Proteoglycans / Q000648:ultrastructure; D011817:Rabbits; D012160:Retina / Q000187:drug effects / Q000648:ultrastructure; D014822:Vitreous Body / Q000187:drug effects* / Q000648:ultrastructure
D005466:Fluorocarbons; D011509:Proteoglycans; D003094:Collagen; C042852:perflutren; D006821:Hyaluronoglucosaminidase