Glucose-induced dysmorphogenesis in the cultured rat conceptus: prevention by supplementation with myo-inositol.
Growth retardation and dysmorphogenesis in the rat conceptus are accompanied by diminished tissue myo-inositol following culture from day 9.5 to 11.5 of development in the presence of increased amounts of glucose. Reductions of myo-inositol and increased malformations are not corrected by aldose reductase inhibitors. In contrast, supplementation of culture medium with myo-inositol (1.5 mg/ml) restores tissue myo-inositol content, lowers the incidence of dysmorphogenic embryos (from 51.1 to 28.6%, P less than 0.001) and reduces the incidence of neural tube defects to control levels (from 33.3 to 6%, P less than 0.001). These results suggest that myo-inositol depletion is involved in the mechanism of diabetic embryopathy.
Hod
M
M
Center for Endocrinology, Metabolism and Nutrition, Northwestern University Medical School, Chicago, IL.
Star
S
S
Passonneau
J
J
Unterman
T G
TG
Freinkel
N
N
D000818:Animals; D000013:Congenital Abnormalities / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D003470:Culture Media; D046508:Culture Techniques; D004195:Disease Models, Animal; D004622:Embryo, Mammalian / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D006943:Hyperglycemia / Q000150:complications / Q000378:metabolism; D007294:Inositol / Q000378:metabolism / Q000494:pharmacology*; D011247:Pregnancy; D011254:Pregnancy in Diabetics / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013012:Sorbitol / Q000378:metabolism
D003470:Culture Media; D007294:Inositol; D013012:Sorbitol; D005947:Glucose