title: Occurrence and developmental consequences of aberrant cellular organization in meiotically mature human oocytes after exogenous ovarian hyperstimulation.
abstract: The extent to which fertilization failure in in vitro inseminated human oocytes obtained from hyperstimulated ovaries was associated with alterations in cellular structure and cytoplasmic organization was examined by a combination of DIC, fluorescence, time-lapse video, and transmission electron microscopy. Detailed analysis of meiotically mature preovulatory (uninseminated) oocytes, and of oocytes that failed to fertilize in vitro, indicated that between 10% and 15% of grossly normal-appearing MII-stage oocytes displayed one of the following cellular perturbations: 1) a subtle change in organelle distribution, 2) a small region(s) of intracellular cytolysis, 3) a massive aggregation of SER tubules, 4) an accumulation of vesicles presumed to be of SER origin, 5) a change in the structural organization of the cortical cytoplasm and overlying plasma membrane, 6) a sudden and rapid internalization of perivitelline fluid by means of an apparently aberrant process of endocytosis, and 7) a premature and partial exocytosis of cortical granules. The description of these disorders is discussed with respect to the developmental consequences for the oocyte.
authors: Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder 80309.
authors: J
authors: J
authors: Van Blerkom
mesh_terms: D015550:Cell Degranulation; D003593:Cytoplasm / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D005089:Exocytosis; D005306:Fertilization; D005307:Fertilization in Vitro; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008540:Meiosis; D008677:Metaphase; D008856:Microscopy, Fluorescence; D008857:Microscopy, Interference; D008871:Microvilli / Q000648:ultrastructure; D009865:Oocytes / Q000166:cytology* / Q000648:ultrastructure; D015388:Organelles / Q000648:ultrastructure; D014617:Vacuoles / Q000648:ultrastructure; D014742:Videodisc Recording