Genomic divergence of an HIV-2 from a German AIDS patient probably infected in Mali.
The complete nucleotide sequence of an HIV-2 isolate derived from a German AIDS patient with predominantly neurological symptoms is reported. The HIV-2BEN sequence is highly divergent from those of previously described HIV-2 and SIV strains. Evolutionary tree analysis of eight HIV-2 sequences reveals the existence of three HIV-2 groups. HIV-2BEN belongs to a group with two isolates from Ghana and The Gambia. Based on a comparison of HIV-2BEN with six HIV-2 isolates, SIVsmm and SIVmac, the variability of the structural env and gag proteins is similar within the HIV-2/SIVsmm/mac and HIV-1 groups. In contrast, the regulatory HIV-1 proteins are more highly conserved than those from HIV-2 strains. Multiple sequence alignments reveal that some domains of the envelope and regulatory proteins are well conserved among HIV-1, HIV-2/SIVsmm/mac, SIVagm and SIVmnd. The identification of conserved domains within the external glycoprotein could help to develop broadly active vaccines.
Kirchhoff
F
F
German Primate Center, Department of Virology and Immunology, Göttingen.
Jentsch
K D
KD
Stuke
A
A
Mous
J
J
Hunsmann
G
G
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D000595:Amino Acid Sequence; D001483:Base Sequence; D015686:Gene Products, env / Q000737:chemistry / Q000235:genetics; D015683:Gene Products, gag / Q000737:chemistry / Q000235:genetics; D015693:Gene Products, nef / Q000737:chemistry / Q000235:genetics; D015684:Gene Products, pol / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D015498:HIV-2 / Q000145:classification / Q000235:genetics*; D006801:Humans; D008302:Mali; D008969:Molecular Sequence Data; D010802:Phylogeny; D012091:Repetitive Sequences, Nucleic Acid; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D015302:Simian Immunodeficiency Virus / Q000235:genetics; D054334:Viral Regulatory and Accessory Proteins / Q000737:chemistry / Q000235:genetics; D054311:nef Gene Products, Human Immunodeficiency Virus
D015686:Gene Products, env; D015683:Gene Products, gag; D015693:Gene Products, nef; D015684:Gene Products, pol; D054334:Viral Regulatory and Accessory Proteins; D054311:nef Gene Products, Human Immunodeficiency Virus