Whole-cell voltage-clamp study of sodium current in neuroblastoma cells: effects of inhibition of neurite outgrowth by colchicine.
A method is described for the production of large numbers of neurite-free neuroblastoma cells that are especially suitable for studies involving whole-cell voltage clamp. Differentiation in the presence of colchicine yielded cells having abundant sodium channels, highly reproducible peak currents and no space-clamp problems. Treatment with this drug did not alter the electrophysiological properties of the cells. Colchicine might be similarly advantageous in voltage-clamp studies of different ion channels and other types of cultured neurons.
Partlow
L M
LM
Department of Pharmacology, School of Medicine, University of Utah, Salt Lake City 84132.
Karler
R
R
Turkanis
S A
SA
D001369:Axons / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D003078:Colchicine / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D009447:Neuroblastoma; D009474:Neurons / Q000187:drug effects* / Q000648:ultrastructure; D012964:Sodium / Q000502:physiology*; D014407:Tumor Cells, Cultured
D012964:Sodium; D003078:Colchicine