Mutagenicity of adsorbates to a copper-phthalocyanine derivative recovered from municipal river water.
Blue cotton, bearing a covalently bound copper-phthalocyanine derivative capable of adsorbing polycyclic aromatic hydrocarbons (PAHs) over 3 rings, was applied to recover mutagens from the Katsura River which is a tributary of the Yodo River. The Ames Salmonella/microsome assay with TA98 and TA100 of the blue cotton concentrate recovered from the river water demonstrated indirect mutagenicity toward TA98. The subfractions separated by Sephadex G-25 gel chromatography also showed direct mutagenicity in strains YG1021 and YG1024, the nitroreductase- and O-acetyltransferase-overproducing derivatives of TA98; this activity was greatly increased by the addition of S9 mix, especially in YG1024. However, these subfractions were less mutagenic with TA98NR or TA98/1,8-DNP6, regardless of whether S9 mix was present or not. The behaviors of these mutagenic activities therefore suggested that frameshift mutagens of both directly mutagenic nitroarenes and indirectly mutagenic aminoarenes were present in the blue cotton concentrate from the river water.
Sayato
Y
Y
Division of Environmental Health, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.
Nakamuro
K
K
Ueno
H
H
Goto
R
R
D000327:Adsorption; D002850:Chromatography, Gel; D007202:Indicators and Reagents; D007211:Indoles; D007564:Japan; D009152:Mutagenicity Tests; D009153:Mutagens / Q000737:chemistry / Q000302:isolation & purification*; D009942:Organometallic Compounds; D011083:Polycyclic Compounds / Q000302:isolation & purification* / Q000633:toxicity; D012486:Salmonella typhimurium / Q000187:drug effects; D014874:Water Pollutants, Chemical / Q000302:isolation & purification / Q000633:toxicity*; D014881:Water Supply / Q000032:analysis*
D007202:Indicators and Reagents; D007211:Indoles; D009153:Mutagens; D009942:Organometallic Compounds; D011083:Polycyclic Compounds; D014874:Water Pollutants, Chemical; C015445:copper phthalocyanine