title: Functional activity of ciliated outgrowths from cultured human nasal and tracheal epithelia.
abstract: Primary cultures of respiratory epithelium were produced as outgrowths from human fetal and adult tracheal and nasal polyp explants. Video recordings of the epithelial cell outgrowths were carried out after 5 days of culture and the ciliary beating frequency was analyzed by using a video technique. Uniform fields of differentiated ciliated cells were observed near the edge of the explant. In the transition region of the outgrowth from the explant to the outgrowth periphery, isolated ciliated cells were present, as well as cells with fused cilia. The ciliary beating frequency of the outgrowth of well-differentiated ciliated cells (13.5 +/- 1.4 Hz) was significantly higher (p less than 0.001) than the beating frequency of both the explant (11.9 +/- 0.7 Hz) and the ciliated cells with fused cilia (9.8 +/- 1.7 Hz). The same differentiation stages and functional activities were observed in the outgrowth cultures, whatever their origin. These in vitro models are comparable with each other and therefore could be useful for studying the ciliogenesis and functional activity of the human respiratory epithelium.
authors: INSERM U.314, CHR Maison-Blanche, Reims, France.
authors: J M
authors: JM
authors: Zahm
authors: D
authors: D
authors: Pierrot
authors: J
authors: J
authors: Hinnrasky
authors: C
authors: C
authors: Fuchey
authors: M
authors: M
authors: Chevillard
authors: D
authors: D
authors: Gaillard
authors: E
authors: E
authors: Puchelle
mesh_terms: D002923:Cilia / Q000502:physiology* / Q000648:ultrastructure; D046508:Culture Techniques / Q000379:methods; D004848:Epithelium / Q000502:physiology; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009068:Movement / Q000502:physiology; D009298:Nasal Polyps / Q000503:physiopathology; D009666:Nose / Q000502:physiology*; D014132:Trachea / Q000196:embryology / Q000502:physiology*; D014741:Video Recording