The VM model of glioma: preparation of multicellular tumour spheroids (MTS) and their response to chemotherapy.
A cell line, 497-P(1), derived from the VM spontaneous murine astrocytoma has been used to develop an in vitro therapeutic model of human glioma. In this study we describe the preparation of MTS from this cell line. The in vitro chemosensitivity of 497-P(1) MTS has been examined and compared to the sensitivity of the monolayer culture. BCNU and CCNU both produced growth delay in MTS at doses below the ID50 of the monolayer culture. MTS, however, were considerably more resistant to vincristine and procarbazine when compared to the monolayer culture.
Bradford
R
R
Gough-Cooper Department of Neurological Surgery, Institute of Neurology, Queen Square, London, United Kingdom.
Darling
J L
JL
Sier
N
N
Thomas
D G
DG
D000818:Animals; D002330:Carmustine / Q000627:therapeutic use; D002455:Cell Division / Q000187:drug effects; D004195:Disease Models, Animal; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D005910:Glioma / Q000188:drug therapy*; D008130:Lomustine / Q000627:therapeutic use; D051379:Mice; D011344:Procarbazine / Q000627:therapeutic use; D014407:Tumor Cells, Cultured / Q000187:drug effects*; D014750:Vincristine / Q000627:therapeutic use
D011344:Procarbazine; D014750:Vincristine; D008130:Lomustine; D002330:Carmustine