title: Crystal structure of an HIV-binding recombinant fragment of human CD4.
abstract: CD4 glycoprotein on the surface of T cells helps in the immune response and is the receptor for HIV infection. The structure of a soluble fragment of CD4 determined at 2.3 A resolution reveals that the molecule has two intimately associated immunoglobulin-like domains. Residues implicated in HIV recognition by analysis of mutants and antibody binding are salient features in domain D1. Domain D2 is distinguished by a variation on the beta-strand topologies of antibody domains and by an intra-sheet disulphide bridge.
authors: Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032.
authors: S E
authors: SE
authors: Ryu
authors: P D
authors: PD
authors: Kwong
authors: A
authors: A
authors: Truneh
authors: T G
authors: TG
authors: Porter
authors: J
authors: J
authors: Arthos
authors: M
authors: M
authors: Rosenberg
authors: X P
authors: XP
authors: Dai
authors: N H
authors: NH
authors: Xuong
authors: R
authors: R
authors: Axel
authors: R W
authors: RW
authors: Sweet
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D001665:Binding Sites; D005075:Biological Evolution; D015704:CD4 Antigens / Q000648:ultrastructure*; D003196:Computer Graphics; D003461:Crystallography; D004252:DNA Mutational Analysis; D015699:HIV Envelope Protein gp120 / Q000378:metabolism*; D006681:HLA-D Antigens / Q000378:metabolism; D008561:Membrane Fusion; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010446:Peptide Fragments; D011487:Protein Conformation; D011994:Recombinant Proteins; D013329:Structure-Activity Relationship; D014961:X-Ray Diffraction
chemical_list: D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D015699:HIV Envelope Protein gp120; D006681:HLA-D Antigens; D010446:Peptide Fragments; D011994:Recombinant Proteins