Radical intermediates in veratryl alcohol oxidation by ligninase. NMR evidence.
Proton nuclear magnetic resonance (NMR) spectra of veratryl alcohol (3,4-dimethoxybenzyl alcohol) were obtained during its oxidation by ligninase. It was observed that a substantial increase in the linewidths of the resonances occurred only in the presence of both the enzyme and hydrogen peroxide. Quenching the reaction by the addition of alkali immediately restored the normal linewidths of the resonances. Furthermore, inversion-recovery experiments showed a decrease in the longitudinal relaxation time of the substrate when the enzyme was actively turning over. Changes in both these NMR parameters are consistent with the generation of radical intermediates during the ligninase-catalysed oxidation of veratryl alcohol.
Gilardi
G
G
Centre for Biotechnology, Imperial College of Science, Technology and Medicine, South Kensington, London.
Harvey
P J
PJ
Cass
A E
AE
Palmer
J M
JM
D000363:Agaricales / Q000201:enzymology; D001592:Benzyl Alcohols / Q000378:metabolism*; D005609:Free Radicals; D006859:Hydrogen; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010105:Oxygenases / Q000378:metabolism*
D001592:Benzyl Alcohols; D005609:Free Radicals; D006859:Hydrogen; D010105:Oxygenases; C044391:ligninase; C042197:veratryl alcohol