Parasite-induced processes for adenosine permeation in mouse erythrocytes infected with the malarial parasite Plasmodium yoelii.
In mouse erythrocytes harbouring the malarial parasite Plasmodium yoelii, three processes contributed to inward fluxes of adenosine, one of which is attributed to the native nucleoside transporter, because of the inhibitory effects of nitrobenzylthioinosine (NBMPR). New (parasite-induced) permeation processes of low NBMPR-sensitivity were (i) saturable fluxes with preference for the D enantiomer (D-Ado) and (ii) apparently unsaturable fluxes that proceeded by a channel-like route without enantiomeric selectivity. Parasite-induced fluxes of L- and D-Ado were similarly inhibited by furosemide [IC50 (concn. causing half-maximal inhibition) 15-17 microM], whereas D-Ado fluxes in uninfected erythrocytes were 10-fold less sensitive.
Gati
W P
WP
McEachern Laboratory, University of Alberta, Edmonton, Canada.
Lin
A N
AN
Wang
T I
TI
Young
J D
JD
Paterson
A R
AR
D000241:Adenosine / Q000097:blood*; D000818:Animals; D001692:Biological Transport; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism* / Q000469:parasitology; D005260:Female; D005665:Furosemide / Q000494:pharmacology; D007700:Kinetics; D008288:Malaria / Q000097:blood*; D051379:Mice; D008813:Mice, Inbred ICR; D010967:Plasmodium yoelii / Q000472:pathogenicity*; D012016:Reference Values
D005665:Furosemide; D000241:Adenosine