High-performance liquid affinity chromatography for the purification of immunoglobulin A from human serum using jacalin.
A high-performance liquid affinity chromatographic method for the purification of serum immunoglobulin A (IgA) using a jacalin column is described. The automated procedure takes about 2 with minimal manipulation. The yields of the isolated IgA and of its IgG and IgM contamination were studied by enzyme-linked immunosorbent assay (ELISA) of 30 sera. Purity was assured by immunoelectrophoresis. The ratio of IgA1 to total IgA was unchanged after purification, as verified by ELISA. The results showed that greater than 90% IgA could be recovered with less than 0.5% total IgG and greater than 2.0% total IgM remaining in the fractions containing purified IgA.
Chui
S H
SH
Department of Health Sciences, Hong Kong Polytechnic, Kowloon.
Lam
C W
CW
Lewis
W H
WH
Lai
K N
KN
D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007122:Immunoelectrophoresis; D007070:Immunoglobulin A / Q000302:isolation & purification*; D007075:Immunoglobulin M / Q000302:isolation & purification; D037102:Lectins; D037121:Plant Lectins
D007070:Immunoglobulin A; D007075:Immunoglobulin M; D037102:Lectins; D037121:Plant Lectins; C043696:jacalin