In vitro metabolism of progesterone, 17-hydroxyprogesterone, and 17,20 beta-dihydroxy-4-pregnen-3-one by ovaries of the common carp Cyprinus carpio: production rates of polar metabolites.
[14C]Progesterone, 17-[3H]hydroxyprogesterone, and 17,20 beta-[3H]dihydroxy-4-pregnen-3-one (17,20 beta P) were incubated for 1, 3, 6 or 24 hr with ovaries from carp which had received injections of either carp pituitary extract or saline. All three substrates were very rapidly metabolized to polar products, but 17,20 beta P was not detected as a metabolite of either progesterone or 17-hydroxyprogesterone. The major metabolite in all incubations was very similar, but not identical, in chemical and chromatographic properties to 5 beta-pregnane-3 alpha, 6 alpha, 17,20 beta-tetrol. A compound isopolar with 5 beta-pregnane-3 alpha, 6 alpha, 17,20 alpha-tetrol was isolated only in incubations of progesterone and 17-hydroxyprogesterone. It is suggested that hydroxylation and reduction may be a deactivation mechanism allowing 17,20 beta P to build up as an intermediate, and active maturation inducing steroid, only in response to the ovulatory gonadotrophin surge, thus assisting in synchrony of oocyte maturation.
Kime
D E
DE
Department of Animal and Plant Sciences, The University, Sheffield, United Kingdom.
D019326:17-alpha-Hydroxyprogesterone; D000818:Animals; D002347:Carps / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D005260:Female; D005965:Glucuronates / Q000378:metabolism; D006908:Hydroxyprogesterones / Q000378:metabolism* / Q000493:pharmacokinetics; D066298:In Vitro Techniques; D009865:Oocytes / Q000187:drug effects; D009866:Oogenesis / Q000187:drug effects; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism* / Q000493:pharmacokinetics
D005965:Glucuronates; D006908:Hydroxyprogesterones; C007433:17,20-dihydroxy-4-pregnen-3-one; D011374:Progesterone; D019326:17-alpha-Hydroxyprogesterone