Development of an in vitro model of primate cervical goblet cells.
Mucins, are densely packed in secretory granules of goblet cells. Upon exocytosis they undergo massive hydration that results in the formation of the mucus gel. Because the mucin polymer network is held together by tangles and low energy bonds, the rheological properties of this gel are mainly determined by the degree of postexocytotic hydration. Hydration in mucus is governed by a Donnan equilibrium as it is driven by electrostatic interaction among the polyionic charges of the mucins and other fixed polyions. Although, variations of charge density of the mucin polymer could be an efficient physiologic mechanism to control the rate of mucus hydration and rheology, this subject has not been investigated. In here we describe a primary tissue culture system of cervical goblet cells of the monkey uterus. This preparation allows to measure directly the kinetic of hydration of exocytosed mucins. Because the physicochemical parameters of the bathing medium can be effectively controlled, variations in the kinetic of mucins swelling upon exocytosis, can be used as a convenient indicator of fluctuations of charge density in secretory products. Since the cervical mucosa readily respond to endocrine influences, this preparation can provide a useful model to study the effect of hormones or other transmitters on polyionic charge density of secretory product.
Verdugo
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P
Center for Bioengineering, University of Washington, Seattle 98195.
Langley
L
L
Aitken
M L
ML
Villalon
M
M
D000818:Animals; D002584:Cervix Uteri / Q000166:cytology / Q000378:metabolism*; D046508:Culture Techniques / Q000379:methods; D005089:Exocytosis / Q000502:physiology*; D005260:Female; D008254:Macaca nemestrina; D008954:Models, Biological; D009077:Mucins / Q000502:physiology*; D009092:Mucous Membrane / Q000166:cytology*; D014741:Video Recording
D009077:Mucins