Usefulness of MC-540 fluorescent dye as probe versus scanning electron microscopy for assessing membrane changes.
The effect of primaquine enantiomers on cell membranes of glucose-6-phosphate (G-6PD)-deficient erythrocytes was studied in vitro. Staining with merocyanine (Mc-540) showed that exposure to primaquine enantiomers produces significant fluorescence in G-6PD-deficient erythrocytes, indicating marked drug-induced alterations in membrane fluidity. Scanning electron microscopy (SEM) studies confirmed that primaquine enantiomers altered membrane morphology (by producing stomatocytes) in both normal and G-6PD-deficient cells. The concentration-dependent effect, however, was more pronounced with MC-540, a lipophylic dye, than with SEM (an expensive technique).
Agarwal
S
S
Sanjay Gandhi Postgraduate Institute of Medical Sciences, Department of Genetics and Immunology, Lucknow, India.
Khardori
R
R
Agarwal
S S
SS
D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism*; D005456:Fluorescent Dyes; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates / Q000097:blood / Q000172:deficiency*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D011319:Primaquine / Q000494:pharmacology; D011744:Pyrimidinones
D005456:Fluorescent Dyes; D005958:Glucosephosphates; D011744:Pyrimidinones; D019298:Glucose-6-Phosphate; C003954:merocyanine dye; D011319:Primaquine