Isoenzymes of alkaline phosphatases in seminomas. An immunohistochemical and biochemical study.
Placental alkaline phosphatase (the PLAP-like isoenzyme) and liver alkaline phosphatase (LAP) were demonstrated immunohistochemically by use of monoclonal antibodies in the tumor cells of twelve seminomas and one seminoma metastasis. Intestinal alkaline phosphatase (IAP) was not found. The PLAP-like and LAP enzymes showed high catalytic activities compared to normal testis. This is the first occasion that LAP has been demonstrated by immunochemistry in seminoma cells. The results suggest that demonstration of these tumor enzymes may be useful markers for seminomas in histopathological specimens.
Brehmer-Andersson
E
E
Department of Pathology, Södersjukhuset, Stockholm, Sweden.
Ljungdahl-Ståhle
E
E
Koshida
K
K
Yamamoto
H
H
Stigbrand
T
T
Wahren
B
B
D000469:Alkaline Phosphatase / Q000032:analysis* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D014408:Biomarkers, Tumor / Q000032:analysis; D004407:Dysgerminoma / Q000201:enzymology* / Q000473:pathology / Q000556:secondary; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007527:Isoenzymes / Q000032:analysis* / Q000276:immunology; D008099:Liver / Q000201:enzymology; D008297:Male; D010920:Placenta / Q000201:enzymology; D013736:Testicular Neoplasms / Q000201:enzymology* / Q000473:pathology / Q000556:secondary
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D007527:Isoenzymes; D000469:Alkaline Phosphatase