Interactions of PRP2 protein with pre-mRNA splicing complexes in Saccharomyces cerevisiae.
PRP2 protein of Saccharomyces cerevisiae is required for the pre-mRNA splicing reaction but not for the early stages of spliceosome assembly. Using anti-PRP2 antibodies we demonstrate that PRP2 protein is associated with spliceosomes prior to, and throughout step 1 of the splicing reaction. Heat-inactivated prp2 protein, by contrast, does not seem to associate with spliceosomes. By elution of electrophoretically distinct spliceosomal complexes from non-denaturing gels we identify the specific complex with which PRP2 initially interacts in the pathway of spliceosome assembly.
King
D S
DS
Institute of Cell and Molecular Biology, University of Edinburgh, UK.
Beggs
J D
JD
D000906:Antibodies / Q000276:immunology; D001483:Base Sequence; D005656:Fungal Proteins / Q000276:immunology / Q000378:metabolism*; D006358:Hot Temperature; D007700:Kinetics; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D012322:RNA Precursors / Q000378:metabolism*; D012326:RNA Splicing; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D000906:Antibodies; D005656:Fungal Proteins; D012322:RNA Precursors