title: The intracellular distribution of cobalt in exposed and unexposed rat myocardium.
abstract: The intracellular distribution of cobalt was analysed in the myocardium of exposed and unexposed rats. The exposed rats were given a dietary cobalt supplementation of 40 mg CoSO4.7 H2O/kg body weight for 8 weeks. The mitochondrial fraction showed the greatest relative increase in cobalt: 0.09 ng/mg protein in the unexposed rats to 8.43 ng/mg protein in the exposed rats. In the exposed rats the submitochondrial particles had the highest levels of cobalt: 19.43 ng/mg protein, followed by the sarcoplasmatic reticulum: 12.3 ng/mg protein. The microsomal 44,000 g supernatant also showed an increase, although the levels remained low (0.51 ng/mg protein in the exposed animals). Apparently the calcium-storing organelles had the highest levels of cobalt. This could affect calcium flux in myocardial cells and, secondarily, tension development in cardiac muscle.
authors: Department of Medicine, Karolinska Hospital, Stockholm, Sweden.
authors: N
authors: N
authors: Clyne
authors: B
authors: B
authors: Persson
authors: N
authors: N
authors: Havu
authors: E
authors: E
authors: Hultman
authors: L E
authors: LE
authors: Lins
authors: S K
authors: SK
authors: Pehrsson
authors: J
authors: J
authors: Rydström
authors: R
authors: R
authors: Wibom
mesh_terms: D000818:Animals; D003035:Cobalt / Q000493:pharmacokinetics*; D004032:Diet; D006321:Heart / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D008861:Microsomes / Q000378:metabolism; D008929:Mitochondria, Heart / Q000378:metabolism; D009200:Myocardial Contraction / Q000187:drug effects; D009206:Myocardium / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012519:Sarcoplasmic Reticulum / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D011506:Proteins; D003035:Cobalt