title: Intragranular co-storage of neuropeptide Y and arginine vasopressin in the paraventricular magnocellular neurons of the rat hypothalamus.
abstract: Certain populations of arginine vasopressin (AVP) neurons in the magnocellular paraventricular nucleus became immunoreactive for neuropeptide Y (NPY) when rats were treated with colchicine or monosodium glutamate (MSG). The co-storage of these peptides was examined by employing a post-embedding electron-microscopic immunohistochemistry technique using gold-labeled antibodies to the two peptides. In colchicine-treated rats, the neuronal perikarya contained numerous secretory granules showing co-storage of the two peptides. The cells of the MSG-treated rats were characterized by having well-developed Golgi bodies with the granular structures also co-storing the two peptides, although the secretory granules in the perikarya were rather fewer than in the colchicine-treated rats. It is concluded that the destruction of the arcuate nucleus by MSG-treatment may potentiate the synthesis of NPY in AVP neurons, the synthesis of which is latent in intact animals.
authors: Department of Anatomy, School of Medicine, University of Tokushima, Japan.
authors: Y
authors: Y
authors: Kagotani
authors: S
authors: S
authors: Hisano
authors: Y
authors: Y
authors: Tsuruo
authors: S
authors: S
authors: Daikoku
authors: Y
authors: Y
authors: Okimura
authors: K
authors: K
authors: Chihara
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001127:Arginine Vasopressin / Q000378:metabolism*; D003078:Colchicine / Q000494:pharmacology; D003594:Cytoplasmic Granules / Q000187:drug effects / Q000648:ultrastructure; D008297:Male; D009478:Neuropeptide Y / Q000378:metabolism*; D010286:Paraventricular Hypothalamic Nucleus / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D012970:Sodium Glutamate / Q000494:pharmacology
chemical_list: D009478:Neuropeptide Y; D001127:Arginine Vasopressin; D003078:Colchicine; D012970:Sodium Glutamate