Transient increase of raf protein kinase-like immunoreactivity in the rat dentate gyrus during long-term potentiation.
Altered levels of cellular raf proteins (products of the raf protooncogenes) have been shown in the neurons of the dentate fascia of rats in response to high-frequency stimulation, with light microscopic immunohistochemistry by using polyclonal antibodies. No raf-1-like staining was seen in unstimulated tissue, while the pan-raf antibodies revealed immunoreactivity in the cytoplasm of neurons in the Ammon's horn and dentate fascia of rats and guinea pigs. The induction of long-term potentiation in the dentate fascia of freely-moving rats triggered the appearance of raf-1-like staining and increased the number of granule cells with pan-raf-like immunoreactivity. Since these proteins are serine/threonine-specific protein kinases, their appearance in long-term potentiation may indicate the activation of important cell membrane - nucleus transduction pathways.
Mihály
A
A
Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Göttingen, F.R.G.
Oláh
Z
Z
Krug
M
M
Kuhnt
U
U
Matthies
H
H
Rapp
U R
UR
Joó
F
F
D000818:Animals; D004558:Electric Stimulation; D006624:Hippocampus / Q000166:cytology / Q000201:enzymology / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D019908:Proto-Oncogene Proteins c-raf; D011712:Pyramidal Tracts / Q000166:cytology / Q000201:enzymology / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains
D011518:Proto-Oncogene Proteins; D011505:Protein-Tyrosine Kinases; D019908:Proto-Oncogene Proteins c-raf