title: Molecular cloning of the large subunit of transforming growth factor type beta masking protein and expression of the mRNA in various rat tissues.
abstract: Masking protein (MP), which neutralizes the activity of transforming growth factor type beta 1 (TGF-beta 1), is composed of a dimeric N-terminal part of a TGF-beta 1 precursor of Mr 39,000 and an unknown large subunit of Mr 105,000-120,000. The deduced primary structure of the MP large subunit was elucidated by determining the nucleotide sequence of its cDNA. The cDNA encodes a prepro-precursor of 1712 amino acid residues with a calculated Mr of 186,596. The mature large subunit seems to be derived proteolytically from a prepro-precursor and the calculated Mr is 91,606. The precursor has seven N-linked glycosylation sites and an unusual structure containing 18 epidermal growth factor-like domains and four cysteine-rich internal repeats. The large subunit mRNA is synthesized in parallel with the expression of TGF-beta 1 mRNA in various rat tissues.
authors: Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan.
authors: T
authors: T
authors: Tsuji
authors: F
authors: F
authors: Okada
authors: K
authors: K
authors: Yamaguchi
authors: T
authors: T
authors: Nakamura
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D015870:Gene Expression; D053779:Latent TGF-beta Binding Proteins; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010446:Peptide Fragments / Q000737:chemistry; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D014018:Tissue Distribution; D016212:Transforming Growth Factor beta / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D053779:Latent TGF-beta Binding Proteins; D015345:Oligonucleotide Probes; D010446:Peptide Fragments; D011498:Protein Precursors; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta