Inhibition of nuclear T3 binding via PLA2-induced release of fatty acids from nuclear membranes.
This study was undertaken to explore putative regulatory mechanisms involved in the inhibition of nuclear T3 binding (INB) by fatty acids. Ether extracts of intact rat liver nuclei contained INB-activity. Removement of the nuclear membrane resulted in the loss of INB-activity of the nuclei. Incubation of intact nuclei with phospholipase A2 increased nuclear INB-activity in a time- and dose-dependent manner; this was correlated with a rise of free fatty acid concentration in the ether extract. We conclude that fatty acids present in the nuclear membrane can be released by phospholipase A2, and are capable of inhibiting nuclear T3 binding.
van der Klis
F R
FR
University of Amsterdam, The Netherlands.
Wiersinga
W M
WM
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000378:metabolism* / Q000494:pharmacology; D008297:Male; D009685:Nuclear Envelope / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D051381:Rats; D011919:Rats, Inbred Strains; D011988:Receptors, Thyroid Hormone / Q000187:drug effects / Q000378:metabolism; D014284:Triiodothyronine / Q000378:metabolism*
D005230:Fatty Acids, Nonesterified; D011988:Receptors, Thyroid Hormone; D014284:Triiodothyronine; D010741:Phospholipases A; D054467:Phospholipases A2