Comparison of pre-S1 and pre-S2 proteins in hepatocytes with replication by in situ hybridization assay.
To compare the relationship of pre-S1 and pre-S2 proteins in hepatocytes with HBV replication, HBVDNA in situ hybridization was performed for a group of patients with chronic hepatitis by bio-probe, in conjunction with detection of intrahepatic HBsAg, HBcAg, pre-S1 and-S2 antigens. It was found that the expression of intrahepatic pre-s1 and pre-S2 proteins was visualized as cytoplasmic homogeneous and inclusion types, on some occasions as membranous localization. However, the positive rate for pre-S1 protein was up to 75% (15/20), being significantly higher than that of pre-S2 protein (35%; 7/20) in the same group (P less than 0.05). What in more important, the pre-S1 expression, rather than pre-s2, was in parallel with the presence of intrahepatic HBVDNA and HbcAg. All these findings suggest that the pre-S1 protein might be mainly expressed at the HBV replication phase, thus, to some extent, the detection of pre-S1 protein might complement HBe system and HBVDNA clinical routine.
Zhang
Y Y
YY
Clinical Immunology Research Unit, Tongji Hospital, Tongji Medical University, Wuhan.
Yu
Z Q
ZQ
Wan
Y K
YK
Yan
P
P
Hao
L J
LJ
D015415:Biomarkers; D004279:DNA, Viral / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis*; D006509:Hepatitis B / Q000378:metabolism*; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006513:Hepatitis B e Antigens / Q000032:analysis; D006515:Hepatitis B virus / Q000502:physiology; D006521:Hepatitis, Chronic / Q000378:metabolism; D006801:Humans; D011498:Protein Precursors / Q000032:analysis*; D017293:Protein S; D014779:Virus Replication
D015415:Biomarkers; D004279:DNA, Viral; D006023:Glycoproteins; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D011498:Protein Precursors; D017293:Protein S; C050930:protein S precursor