Localization on pig chromosome 6 of markers GPI, APOE, and ENO1, carried by human chromosomes 1 and 19, using in situ hybridization.
In the pig, the linkage group around the halothane gene (HAL), composed of S-GPI-HAL-H-A1BG-PGD, has been assigned to bands p1.2----q2.2 of chromosome 6. In man, ENO1-PGD and APOE-GPI constitute two syntenic groups situated on different chromosomes (1 and 19, respectively). Since GPI and PGD are linked in the pig, we have hybridized the human cDNA probes for ENO1 and APOE to pig chromosomes. These markers were assigned to pig chromosome 6, in the q2.2----q2.4 and cen----q2.1 regions, respectively, using in situ hybridization. Since GPI and APOE are situated in the same region, we combined the use of high resolution chromosome analysis and in situ hybridization to give a more precise localization in the q1.2 and q1.2----q2.1.2 regions of chromosome 6. A possible linear order of these genes is proposed.
Yerle
M
M
I.N.R.A., Centre de Recherches de Toulouse, Laboratoire de Génétique Cellulaire Castanet-Tolosan France.
Gellin
J
J
Dalens
M
M
Galman
O
O
D000818:Animals; D001057:Apolipoproteins E / Q000235:genetics; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D002888:Chromosomes, Human, Pair 19; D008040:Genetic Linkage; D005819:Genetic Markers; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics; D006221:Halothane / Q000494:pharmacology*; D006801:Humans; D009693:Nucleic Acid Hybridization; D010734:Phosphogluconate Dehydrogenase / Q000235:genetics; D010751:Phosphopyruvate Hydratase / Q000235:genetics; D013552:Swine / Q000235:genetics*
D001057:Apolipoproteins E; D005819:Genetic Markers; D010734:Phosphogluconate Dehydrogenase; D010751:Phosphopyruvate Hydratase; D005956:Glucose-6-Phosphate Isomerase; D006221:Halothane