Exon trapping: a genetic screen to identify candidate transcribed sequences in cloned mammalian genomic DNA.
Identification and recovery of transcribed sequences from cloned mammalian genomic DNA remains an important problem in isolating genes on the basis of their chromosomal location. We have developed a strategy that facilitates the recovery of exons from random pieces of cloned genomic DNA. The basis of this "exon trapping" strategy is that, during a retroviral life cycle, genomic sequences of nonviral origin are correctly spliced and may be recovered as a cDNA copy of the introduced segment. By using this genetic assay for cis-acting sequences required for RNA splicing, we have screened approximately 20 kilobase pairs of cloned genomic DNA and have recovered all four predicted exons.
Duyk
G M
GM
Department of Pediatrics, University of California, San Francisco 94143-0554.
Kim
S W
SW
Myers
R M
RM
Cox
D R
DR
D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods*; D005091:Exons; D005822:Genetic Vectors; D015698:Genomic Library; D006680:HLA Antigens / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D012326:RNA Splicing
D006680:HLA Antigens