Changes in nucleolar morphology during macrophage development in non-Hodgkin's lymphoma: a morphometric study.
Nucleolar ultrastructure of macrophages developing in suspension culture from monocytes was studied in 20 patients with non-Hodgkin's lymphoma and 20 normal subjects. Morphometric measurements of nucleolar volume, surface area, volume fraction, surface-to-volume ratio and number of profiles per section were made over a 6 day period. Multivariate analysis of variance showed that culture time and subject group had significant effects: changes during macrophage development were less marked in the patient group, nucleoli were fewer, rounder and possibly smaller than normal. The results indicate disturbed macrophage development in non-Hodgkin's lymphoma and may be related to mononuclear phagocyte dysfunction.
Sokol
R J
RJ
Department of Haematology, University of Sheffield, United Kingdom.
D000328:Adult; D000704:Analysis of Variance; D002466:Cell Nucleolus / Q000473:pathology* / Q000648:ultrastructure; D005260:Female; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000473:pathology*; D008264:Macrophages / Q000473:pathology* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D012016:Reference Values