Adaptive response in human lymphocytes conditioned with hydrogen peroxide before irradiation with X-rays.
Cultured human lymphocytes were first exposed to a low 'conditioning' dose of hydrogen peroxide and, subsequently, irradiated with a 'challenge' dose of 1.5 Gy of X-rays in order to analyse the induction of an adaptive response to oxidative damage. A significant reduction in X-ray-induced chromosome damage was evident when H2O2 was given as a single 30 min pulse 24 h after setting up the cultures and the lymphocytes were exposed to X-rays at 48 h. In contrast, when the cells underwent a repeated exposure to H2O2 before irradiation the yield of aberrations was that expected from the combined treatment.
Cortés
F
F
Departamento de Biología Celular, Facultad de Biología, Sevilla, Spain.
Dominguez
I
I
Piñero
J
J
Mateos
J C
JC
D002478:Cells, Cultured; D002842:Chromatids / Q000187:drug effects / Q000528:radiation effects*; D002869:Chromosome Aberrations; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D009153:Mutagens; D014965:X-Rays
D009153:Mutagens; D006861:Hydrogen Peroxide