Effect of tumor necrosis factor/cachectin on the activity of the low density lipoprotein receptor on human skin fibroblasts.
We have investigated the effects of recombinant human tumor necrosis factor (TNF)/cachectin on the cellular binding of human low density lipoprotein (LDL) to human skin fibroblasts. When recombinant TNF was added to cultured cells, LDL binding doubled after 24 h of incubation. The effect of TNF was dose-dependent and its maximal effect was observed at concentrations of 1-10 ng/ml. TNF also stimulated the growth of human skin fibroblasts 1.6-fold. These results indicate that TNF increases LDL-receptor activity, which might be related to its stimulatory effect on cell growth.
Harada
K
K
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Shimano
H
H
Kawakami
M
M
Ishibashi
S
S
Gotoda
T
T
Mori
N
N
Takaku
F
F
Yamada
N
N
D004305:Dose-Response Relationship, Drug; D006801:Humans; D007700:Kinetics; D008077:Lipoproteins, LDL / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000008:administration & dosage / Q000494:pharmacology*
D008077:Lipoproteins, LDL; D014409:Tumor Necrosis Factor-alpha