[Molecular-biological study of vaccinia virus genome. III. Identification of the late gene product protein 36K from Hind-III-P-fragment of vaccinia virus strain L-IVP].
Vaccinia virus gene encoding 36K protein was cloned in pUR290 bacterial expressing vector and resulted in the synthesis of a chimeric protein in E. coli. The chimeric protein consists of beta-galactosidase and virus protein in C-termini. It has virus antigen specificity. By monospecific antibody 36K protein of vaccinia virus was determined to be non-virion. It is localized in the cytoplasm of infected cells.
Riazankina
O I
OI
Shchelkunov
S N
SN
Muravlev
A I
AI
Cheshenko
N V
NV
Chikaev
N A
NA
Malygin
E G
EG
D004279:DNA, Viral / Q000235:genetics; D015247:Deoxyribonuclease HindIII; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D005822:Genetic Vectors; D010957:Plasmids; D011863:Radioimmunoassay; D014616:Vaccinia virus / Q000235:genetics*; D014760:Viral Fusion Proteins / Q000737:chemistry / Q000235:genetics; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*
D004279:DNA, Viral; C066517:Vaccinia virus late protein K36; D014760:Viral Fusion Proteins; D014764:Viral Proteins; D015247:Deoxyribonuclease HindIII
Molekuliarno-biologicheskoe izuchenie genoma virusa ospovaktsiny. III. Identifikatsiia produkta pozdnego gena belka 36K iz HindIII-P-fragmenta genoma virusa ospovaktsiny shtamma L-IVP.