Resistance of platelet proteins to effects of ionizing radiation.
Gamma irradiation of blood components prevents lymphocyte-induced graft-versus-host disease after transfusion in immunocompromised individuals. In this report we demonstrate the resistance of blood platelet proteins to gamma radiation-induced protein cleavage and aggregate formation when platelet concentrates were treated with a dose of 5000 rad. Results of one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis of total platelet protein and cytoskeletal protein preparations indicate that platelet proteins are neither cleaved nor cross-linked under these conditions of irradiation. These results support those of a previous study that documented the lack of any adverse effect of 5000 rad gamma radiation on in vitro platelet properties.
Prodouz
K N
KN
Food and Drug Administration, Center for Biologics Evaluation and Research, Bethesda, MD 20892.
Habraken
J W
JW
Moroff
G
G
D001792:Blood Platelets / Q000528:radiation effects*; D001798:Blood Proteins / Q000032:analysis / Q000528:radiation effects*; D004591:Electrophoresis, Polyacrylamide Gel; D005720:Gamma Rays / Q000009:adverse effects; D006801:Humans
D001798:Blood Proteins