Transcriptional control of the human Harvey ras proto-oncogene: role of multiple elements in the promoter region.
In the promoter region of the human c-Ha-ras1 gene, multiple elements for transcriptional control are dispersed. To examine the role of each element, we made a series of deletion mutants of this promoter region, including internal deletion mutants, and measured the promoter activities of these mutants. Any two of the four Sp1-binding sites upstream from the CCAAT-box element were sufficient for the maximal activity of the c-Ha-ras1 promoter. Either one of the two Sp1-binding sites downstream from the CACCC-box element was also necessary for the maximal promoter activity. Deletion of the CCAAT-box or CACCC-box elements reduced the level of transcription to 25% or 41% of that with the wild-type promoter. These results indicated that at least three Sp1-binding sites, and the CCAAT-box and CACCC-box elements contributed in concert to the activity of the human c-Ha-ras1 promoter.
Nagase
T
T
Tsukuba Life Science Center, Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.
Ueno
Y
Y
Ishii
S
S
D022762:CCAAT-Enhancer-Binding Proteins; D002872:Chromosome Deletion; D004268:DNA-Binding Proteins; D005786:Gene Expression Regulation; D011905:Genes, ras; D006801:Humans; D066298:In Vitro Techniques; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000502:physiology*; D000090063:Proto-Oncogene Mas; D015183:Restriction Mapping; D016329:Sp1 Transcription Factor / Q000502:physiology; D014157:Transcription Factors / Q000502:physiology; D014158:Transcription, Genetic; D014162:Transfection
D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D016329:Sp1 Transcription Factor; D014157:Transcription Factors