A novel flow cytometric method for measuring protein digestion within the phagocytic vacuole of polymorphonuclear neutrophils.
When rhodamine is attached to albumin at a high molar ratio its fluorescence is quenched but fluorescence is released when the protein is digested and the dye released. Using this observation it is possible to measure protein digestion within the phagocytic vacuole of neutrophils. The assay is simple, rapid and measures digestion even in the presence of abnormal phagocytosis.
Haynes
A P
AP
Department of Haematology, Medical Research Centre, City Hospital, Nottingham, U.K.
Fletcher
J
J
Garnett
M
M
Robins
A
A
D000818:Animals; D002474:Cell-Free System; D003571:Cytochalasin B / Q000494:pharmacology; D005434:Flow Cytometry / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D008863:Microspheres; D009504:Neutrophils / Q000502:physiology*; D009895:Opsonin Proteins / Q000502:physiology; D010587:Phagocytosis / Q000187:drug effects / Q000502:physiology*; D011506:Proteins / Q000378:metabolism*; D011817:Rabbits; D012235:Rhodamines / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry
D009895:Opsonin Proteins; D011506:Proteins; D012235:Rhodamines; D012709:Serum Albumin; D003571:Cytochalasin B