Chitin synthetase activity in a developmental mutant of Phycomyces blakesleeanus.
Chitin synthetase activity was analyzed in vitro and in vivo in two morphogenetic stages, namely, dormant spore cells and germlings of the wild type strain and the developmental mutant S356 of Phycomyces blakesleeanus. In vitro experiments showed a much higher specific activity in dormant spores of the mutant strain than in those of the wild-type. This difference was restricted to the dormant spore phase since germlings exhibited comparable levels of activity to those detected in the wild-type strain. Although no correlation was observed between chitin synthesis in vitro and in vivo in mutant spores, germination of these cells was accompanied by an earlier expression of chitin synthetase in vivo. Germination of mutant spores in liquid medium produced morphologically aberrant germlings. Contrary to the extended mycelial growth of the wild-type strain in solid medium, the mutant grew with a typical colonial morphology. Results are discussed in relation to the possible basis of the mutant phenotype.
Ruiz-Flores
E
E
Instituto de Investigacion en Biologia Experimental, Facultad de Quimica, Universidad de Guanajuato, Mexico.
Lopez-Romero
E
E
Gutierrez-Corona
F
F
D002687:Chitin Synthase / Q000096:biosynthesis / Q000235:genetics*; D009024:Morphogenesis; D009154:Mutation; D010641:Phenotype; D010800:Phycomyces / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D013172:Spores, Fungal / Q000502:physiology; D013379:Substrate Specificity
D002687:Chitin Synthase