title: Relaxin-like peptide in ascidians. I. Identification of the peptide and its mRNA in ovary of Herdmania momus.
abstract: The ovaries of the ascidian Herdmania momus were extracted for relaxin. Relaxin immunoactivity was eluted from Sephadex G-50 in the position of authentic porcine ovarian relaxin and these fractions were parallel to porcine relaxin in an homologous porcine relaxin radioimmunoassay. Poly(A)+ RNA from ascidian ovaries hybridized to a 32P-labeled porcine relaxin B-chain 48 mer oligonucleotide probe. There was no hybridization with 32P-labeled probes of equal length but was rather directed to the B-chain of human relaxin or to two different regions of the C-peptide of porcine relaxin. We conclude that the ascidian ovary produces a relaxin-like molecule, possibly with greater homology in the B-chain to mammalian relaxins than in the C-peptide region.
authors: Laboratoire de Biologie Cellulaire Animale, Université J. Fourier, Grenoble I, France.
authors: D
authors: D
authors: Georges
authors: L
authors: L
authors: Tashima
authors: S
authors: S
authors: Yamamoto
authors: G D
authors: GD
authors: Bryant-Greenwood
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002852:Chromatography, Ion Exchange; D005260:Female; D008969:Molecular Sequence Data; D010053:Ovary / Q000378:metabolism*; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis* / Q000302:isolation & purification; D011863:Radioimmunoassay; D012065:Relaxin / Q000096:biosynthesis* / Q000302:isolation & purification; D014561:Urochordata / Q000378:metabolism*
chemical_list: D015347:RNA Probes; D012333:RNA, Messenger; D012065:Relaxin