title: Stimulation of actin synthesis in phalloidin-treated cells. Evidence for autoregulatory control.
abstract: We used the technique of scrape loading to introduce phalloidin into mouse embryo fibroblasts in mass culture. Phalloidin almost completely destroyed actin microfilament bundles, but the amount of polymerized cytoskeleton-associated actin was increased approximately two-fold and the amount of monomeric (Triton X-100 extractable) actin was significantly reduced. The major result of the present study is that the rate of actin synthesis in the phalloidin-treated cells was 2-3 times higher than in the control cells. Northern blot and translation in a cell-free system from rabbit reticulocytes showed that the actin mRNA level significantly increased as a result of phalloidin treatment.
authors: Institute of Protein Research, Academy of Sciences of the USSR, Pushchino, Moscow Region.
authors: A S
authors: AS
authors: Serpinskaya
authors: O N
authors: ON
authors: Denisenko
authors: V I
authors: VI
authors: Gelfand
authors: A D
authors: AD
authors: Bershadsky
mesh_terms: D008841:Actin Cytoskeleton / Q000648:ultrastructure; D000199:Actins / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D066298:In Vitro Techniques; D051379:Mice; D010590:Phalloidine / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D014404:Tubulin / Q000378:metabolism
chemical_list: D000199:Actins; D012333:RNA, Messenger; D014404:Tubulin; D010590:Phalloidine