InsP3- and Ca2(+)-induced Ca2+ release in single mouse oocytes.
To better understand the mechanism of intracellular Ca2+ mobilization, mouse oocytes were micro-injected with 'caged'-inositol-1,4,5 triphosphate caged-InsP3) together with the Ca2+ indicator Fluo-3 to directly induce and monitor Ca2+ redistribution. Photo-released InsP3 elicits [Ca2+]i changes exhibiting several kinetic phases and threshold behaviour. Often Ca2+ oscillations were induced after a single InsP3 pulse. Autoregenerative Ca2+ transients could also be induced by injections of Ca2+ itself, demonstrating unequivocally the presence of a Ca2(+)-induced Ca2(+)-release mechanism in these cells.
Peres
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Dip. di Fisiologia e Biochimica Gen. dell'Universitá di Milano, Italy.
D000818:Animals; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D002451:Cell Compartmentation; D003593:Cytoplasm / Q000502:physiology; D066298:In Vitro Techniques; D015544:Inositol 1,4,5-Trisphosphate / Q000494:pharmacology*; D007700:Kinetics; D008564:Membrane Potentials; D051379:Mice; D009865:Oocytes / Q000378:metabolism*; D010507:Periodicity; D010777:Photochemistry
D015544:Inositol 1,4,5-Trisphosphate; D002118:Calcium