title: Critical role of the C-terminus in the biological activities of human tumour necrosis factor-alpha.
abstract: Alterations of the C-terminal amino acid sequence of human tumour necrosis factor-alpha (hTNF-alpha) caused significant changes in its biological activity. Thus shortening of the C-terminus by removal of two or three amino acids led to a very marked loss of cytotoxic activity. Other, more subtle changes introduced by site-directed mutagenesis resulted in a less drastic reduction in cytotoxicity. The mitogenic activity towards human fibroblasts of the hTNF-alpha was reduced in parallel with the loss of cytotoxicity. These results suggest that the C-terminal amino acids of hTNF-alpha are critical for its biological actions and that they may be part of the receptor-binding site.
authors: Zentralinstitut für Mikrobiologie und Experimentelle Therapie, Akademie der Wissenschaften der DDR, Jena.
authors: K
authors: K
authors: Gase
authors: V G
authors: VG
authors: Korobko
authors: H G
authors: HG
authors: Wisniewski
authors: J
authors: J
authors: Le
authors: V N
authors: VN
authors: Dobrynin
authors: S A
authors: SA
authors: Filippov
authors: W
authors: W
authors: Gutsche
authors: Y N
authors: YN
authors: Maksimova
authors: B
authors: B
authors: Schlott
authors: L N
authors: LN
authors: Shingarova
mesh_terms: D000595:Amino Acid Sequence; D003602:Cytotoxicity, Immunologic; D006801:Humans; D008938:Mitosis / Q000276:immunology; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed / Q000276:immunology; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000737:chemistry* / Q000276:immunology
chemical_list: D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha