The tricarboxylate carrier from rat liver mitochondria. Purification, reconstitution and kinetic characterization.
The tricarboxylate carrier from rat liver mitochondria has been purified and reconstituted into phospholipid vesicles. Its activity has been characterized by both a radioactive citrate uptake assay and a coupled enzymatic assay. A Km of 40 microM and a Vmax of 1.56 mumol x min-1 x mg-1 have been determined for the carrier. Cholesterol levels of between 5-10% of total lipid content are shown to cause a decrease in carrier activity.
Glerum
D M
DM
Institut für Biochemie and Molekularbiologie, Universität Bern, Switzerland.
Claeys
D
D
Mertens
W
W
Azzi
A
A
D000818:Animals; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D002784:Cholesterol / Q000494:pharmacology; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008930:Mitochondria, Liver / Q000378:metabolism*; D051381:Rats
D002352:Carrier Proteins; C020723:citrate-binding transport protein; D002784:Cholesterol