Purification and properties of new ribosome-inactivating proteins with RNA N-glycosidase activity.
Ribosome-inactivating proteins (RIPs) similar to those already known (Stirpe & Barbieri (1986) FEBS Lett. 195, 1-8) were purified from the seeds of Asparagus officinalis (two proteins, asparin 1 and 2), of Citrullus colocynthis (two proteins, colocin 1 and 2), of Lychnis chalcedonica (lychnin) and of Manihot palmata (mapalmin), from the roots of Phytolacca americana (pokeweed antiviral protein from roots, PAP-R) and from the leaves of Bryonia dioica (bryodin-L). The two latter proteins can be considered as isoforms, respectively, of previously purified PAP, from the leaves of P. americana, and of bryodin-R, from the roots of B. dioica. All proteins have an Mr at approx, 30,000, and an alkaline isoelectric point. Bryodin-L, colocins, lychnin and mapalmin are glycoproteins. All RIPs inhibit protein synthesis by a rabbit reticulocyte lysate and phenylalanine polymerization by isolated ribosomes and alter rRNA in a similar manner as the A-chain of ricin and related toxins (Endo et al. (1987) J. Biol. Chem. 262, 5908-5912).
Bolognesi
A
A
Dipartimento di Patologia Sperimentale, Universitá di Bologna, Italy.
Barbieri
L
L
Abbondanza
A
A
Falasca
A I
AI
Carnicelli
D
D
Battelli
M G
MG
Stirpe
F
F
D000595:Amino Acid Sequence; D000818:Animals; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000633:toxicity; D006801:Humans; D066298:In Vitro Techniques; D007526:Isoelectric Point; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009699:N-Glycosyl Hydrolases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000633:toxicity; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000633:toxicity; D014176:Protein Biosynthesis; D012335:RNA, Ribosomal / Q000378:metabolism*; D011817:Rabbits; D054788:Ribosome Inactivating Proteins; D054789:Ribosome Inactivating Proteins, Type 1; D012270:Ribosomes / Q000378:metabolism*
D006023:Glycoproteins; D010940:Plant Proteins; D012335:RNA, Ribosomal; D054789:Ribosome Inactivating Proteins, Type 1; D009699:N-Glycosyl Hydrolases; D054788:Ribosome Inactivating Proteins; C024275:pokeweed antiviral protein