Nerve growth factor rapidly induces expression of the 68-kDa neurofilament gene by posttranscriptional modification in PC12h-R cells.
We have isolated a subclonal cell line of PC12, PC12h-R, which is characterized by its rapid response to nerve growth factor (NGF) in extending its neurites. The mRNA levels of the 68-kDa neurofilament (NF-L) increased severalfold as early as 3 h after the addition of NGF, but this was not accompanied by an increase in the transcription rate. Analysis of the stabilities of 3.5- and 2.3-kilobase (kb) NF-L mRNAs using actinomycin D revealed that 2.3-kb mRNA was degraded much faster than the 3.5-kb species and that NGF apparently stabilized NF-L mRNAs. These results show that the NF-L gene can be regulated posttranscriptionally, a finding that may be important in responding to the acute demand for neurofilament syntheses.
Ikenaka
K
K
Division of Regulation of Macromolecular Functions, Osaka University, Japan.
Nakahira
K
K
Takayama
C
C
Wada
K
K
Hatanaka
H
H
Mikoshiba
K
K
D000310:Adrenal Gland Neoplasms / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D001369:Axons / Q000473:pathology; D003609:Dactinomycin / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D007381:Intermediate Filament Proteins / Q000235:genetics*; D009414:Nerve Growth Factors / Q000494:pharmacology*; D016900:Neurofilament Proteins; D009693:Nucleic Acid Hybridization; D010673:Pheochromocytoma / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D007381:Intermediate Filament Proteins; D009414:Nerve Growth Factors; D016900:Neurofilament Proteins; D012333:RNA, Messenger; C044882:neurofilament protein L; D003609:Dactinomycin