[Detection of polyamines by a new enzymatic differential assay. (7) Fundamental study on a new enzymatic differential assay of tissue polyamines].
The enzymatic method for isolation and determination of urinary polyamines was modified to measure the polyamines in tissue. High recovery rates of polyamines in tissue by the enzymatic method were obtained, namely, 104.5 +/- 7.4% for diamine, 104.7 +/- 23.3% for spermidine and 104.8 +/- 7.5% for spermine. The results obtained by this method correlated well with those obtained by high performance liquid chromatography, and a close correlation was demonstrated for all fractions: diamine r = 0.8991, y = 0.9916x + 0.0499; spermidine r = 0.8936, y = 1.2073x + 0.1005; spermine r = 0.8921, y = 1.2200x-0.2194, (n = 13).
Koide
T
T
Department of Urology, Gifu Prefectural Gifu Hospital.
Sakai
S
S
Kawada
Y
Y
Hara
A
A
Sawada
H
H
D015317:Biogenic Polyamines / Q000032:analysis*; D001681:Biological Assay / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D003959:Diamines / Q000032:analysis; D006801:Humans; D007668:Kidney / Q000737:chemistry; D000587:Oxidoreductases Acting on CH-NH Group Donors; D013095:Spermidine / Q000032:analysis; D013096:Spermine / Q000032:analysis; D000097667:Polyamine Oxidase
D015317:Biogenic Polyamines; D003959:Diamines; D000587:Oxidoreductases Acting on CH-NH Group Donors; D013095:Spermidine; D013096:Spermine; D000097667:Polyamine Oxidase; C022488:putrescine oxidase