title: Blastogenic responses and production of TCGF by mononuclear cells isolated from the bovine endometrium.
abstract: Mononuclear cells (MNCs) were isolated from the endometrium of cattle by enzymatic digestion followed by centrifugation on a density gradient. Of the cells isolated, 32 +/- 3.2% rosetted AET-treated sheep red blood cells, 9 +/- 0.4% possessed surface membrane immunoglobulin, and 15 +/- 6.1% were esterase positive. The cells possessed the ability to proliferate in the presence of T cell mitogens and produced T cell growth factor (TCGF) after in vitro stimulation with Concanavalin A. Endometrial MNCs did not appear to suppress the blastogenesis of peripheral blood MNCs.
authors: Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, Kennett Square 19348.
authors: E D
authors: ED
authors: Watson
authors: H G
authors: HG
authors: Zanecosky
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D002417:Cattle; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D003208:Concanavalin A / Q000494:pharmacology; D004717:Endometrium / Q000166:cytology* / Q000201:enzymology / Q000276:immunology; D004950:Esterases / Q000378:metabolism; D005260:Female; D007376:Interleukin-2 / Q000096:biosynthesis*; D007958:Leukocyte Count / Q000662:veterinary; D007963:Leukocytes, Mononuclear / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D012397:Rosette Formation / Q000662:veterinary
chemical_list: D000954:Antigens, Surface; D007376:Interleukin-2; D010835:Phytohemagglutinins; D003208:Concanavalin A; D004950:Esterases