Effects of five platinum complexes (cis-DDP, trans-DDP, CHIP IV, CBDCA, oxo-Pt) on the induction of sister chromatid exchanges (SCEs) and chromosomal aberrations in Vicia faba root-tip cells.
In a population of plant meristematic cells of Vicia faba the frequency of sister chromatid exchanges (SCEs), incidence of chromosomal aberrations and mitotic activity of cells was evaluated after short-term treatment (1 h) with antitumour active platinum complexes cis-DDP, CHIP IV, CBDCA, oxo-Pt and antitumour inactive trans-DDP. It was found that the action of platinum compounds in equimolar concentration 3.33 microM increases the level of SCEs 1.3 to 6.4-fold. The maximum effect in terms of SCE formation was observed after cis-DDP. Comparison of the incidence of SCEs and chromosomal aberrations in plant cells demonstrated that the tested drugs had a greater effect on SCE formation than on chromosomal aberration induction. Inhibition of mitotic activity correlated with the total cytogenetic damage to chromosomes of Vicia faba cells.
Kuglík
P
P
Institute of Biophysics, Czechoslovak Academy of Sciences, Brno.
Slotová
J
J
Vrána
O
O
D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects*; D002945:Cisplatin / Q000031:analogs & derivatives* / Q000633:toxicity; D007887:Fabaceae / Q000166:cytology / Q000187:drug effects / Q000235:genetics; D008938:Mitosis / Q000187:drug effects; D009153:Mutagens; D009944:Organoplatinum Compounds / Q000633:toxicity*; D010946:Plants, Medicinal; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D009153:Mutagens; D009944:Organoplatinum Compounds; D002945:Cisplatin