Effect of exercise conditioning on excitation-contraction coupling in aged rats.
We studied aged (24-26 mo) Fischer 344 rats after they underwent 8 wk of moderate exercise conditioning. Right ventricular papillary muscles were loaded with the calcium indicator aequorin. Electrophysiological recordings were also performed. Time to peak isometric tension in muscles from exercised aged rats (EAR) was shorter than in those from unexercised aged rats (UAR) (126 +/- 7 vs. 167 +/- 7 ms; P less than 0.01). Time to 50% relaxation from peak isometric tension was also shorter in EAR than in UAR (88 +/- 3 vs. 119 +/- 12 ms; P less than 0.05). There was a trend toward decrease in time to peak light and a significant decrease in time to 50% decline from peak light (33 +/- 4 ms in EAR vs. 59 +/- 17 ms in UAR; P = 0.001). Action potential amplitude was smaller in EAR than in UAR (67 +/- 4 vs. 82 +/- 3 mV; P = 0.003); however, action potential duration was longer (137 +/- 6 ms in EAR vs. 100 +/- 10 ms in UAR; P = 0.005). Right ventricular-to-body weight ratios revealed no evidence of hypertrophy in EAR compared with UAR. Cardiac tissue norepinephrine content was significantly greater in EAR than in UAR (1,212 +/- 25 vs. 630 +/- 105 ng/tissue; P = 0.02). In summary, exercise reversed the age-related prolongation of isometric contraction and associated intracellular calcium transient in the aged rat while it prolonged the transmembrane action potential. In addition, exercise in aged rats resulted in an increase in cardiac norepinephrine content.
Gwathmey
J K
JK
Charles A. Dana Research Institute, Beth Israel Hospital, Boston, Massachusetts.
Slawsky
M T
MT
Perreault
C L
CL
Briggs
G M
GM
Morgan
J P
JP
Wei
J Y
JY
D000331:Aequorin; D000375:Aging / Q000502:physiology*; D000818:Animals; D001835:Body Weight / Q000502:physiology; D002118:Calcium / Q000502:physiology; D002395:Catecholamines / Q000378:metabolism; D007537:Isometric Contraction; D008297:Male; D009206:Myocardium / Q000378:metabolism; D009929:Organ Size / Q000502:physiology; D010210:Papillary Muscles / Q000502:physiology; D010805:Physical Conditioning, Animal; D051381:Rats; D011916:Rats, Inbred F344
D002395:Catecholamines; D000331:Aequorin; D002118:Calcium