Correlations between uptake of technetium, calcium, phosphate, and mineralization in rat tibial bone repair.
Technetium-99m-(99mTc) phosphates are extensively used for detection of bone formation and resorption. The present is a study of 99mTc incorporation during bone remodeling. Uptake of 99mTc-labeled phosphate was studied in an animal model of primary osteogenesis following tibial marrow injury and incorporation was correlated to that of calcium-47 (47Ca), phosphorus-32 (32P), and with matrix vesicle calcification. Isotope uptake on Day 6 in the whole bone was increased compared to controls. On this day, an increase in vesicular diameter and distance from the calcified front was previously observed. Technetium-99m-labeled phosphates were detected only in the organic phase. Phosphorus-32 and 47Ca were detected in both organic and inorganic phases. It is suggested that 99mTc serves as a specific marker to the anabolic phase of remodeling. Increased incorporation of 99mTc during bone healing indicates enhanced organic matrix formation and not calcification.
Shani
J
J
Department of Pharmacology, Hebrew University-Hadassah School of Medicine, Jerusalem, Israel.
Amir
D
D
Soskolne
W A
WA
Schwartz
Z
Z
Chisin
R
R
Sela
J
J
D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D002113:Calcification, Physiologic / Q000502:physiology*; D002132:Calcium Radioisotopes / Q000493:pharmacokinetics*; D010761:Phosphorus Radioisotopes / Q000493:pharmacokinetics*; D051381:Rats; D013667:Technetium / Q000493:pharmacokinetics*; D013977:Tibia / Q000293:injuries* / Q000378:metabolism
D002132:Calcium Radioisotopes; D010761:Phosphorus Radioisotopes; D013667:Technetium