Lectin histochemistry of the central nervous system in a case of feline alpha-mannosidosis.
The diagnosis of feline alpha-mannosidosis is made by demonstrating deficient activity of the enzyme alpha-mannosidase or an elevation of its undergraded substrate in body fluids or tissue. In this study the storage of specific sugar residues in the brain and spinal cord in a case of feline alpha-mannosidosis was examined by means of nine different biotinylated lectins and the avidin-biotin-peroxidase method on formalin-fixed, paraffin-embedded tissue sections. The lectin staining pattern strongly correlated with the known biochemical findings of the stored oligosaccharides in feline alpha-mannosidosis and was different from the lectin reactivity of normal cat tissues. This confirms that lectin histochemistry is a simple, relatively inexpensive and reliable method for diagnosing alpha-mannosidosis in cats.
Castagnaro
M
M
Dipartimento di Patologia Animale, Facoltà di Medicina Veterinaria, Università degli Studi, Turin, Italy.
D000818:Animals; D002371:Cat Diseases / Q000175:diagnosis* / Q000473:pathology; D002415:Cats; D002490:Central Nervous System / Q000737:chemistry*; D002531:Cerebellum / Q000737:chemistry; D002540:Cerebral Cortex / Q000737:chemistry; D006651:Histocytochemistry; D037102:Lectins; D008361:Mannosidases / Q000172:deficiency*; D008526:Medulla Oblongata / Q000737:chemistry; D009474:Neurons / Q000737:chemistry; D013116:Spinal Cord / Q000737:chemistry; D043323:alpha-Mannosidase; D008363:alpha-Mannosidosis / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*
D037102:Lectins; D008361:Mannosidases; D043323:alpha-Mannosidase