title: Extensive heterogeneity of the protein composition of Chlamydia trachomatis following serial passage in two different cell lines.
abstract: To determine if the host-modulated adherence characteristics of the intracellular bacterial pathogen Chlamydia trachomatis were due to the acquisition of altered surface-exposed proteins, highly purified chlamydiae grown in two different host cells were analysed. Two serovars, L1 and E, were grown for multiple passages in both HeLa and McCoy host cells. Numerous protein differences in the chlamydial elementary bodies (EB) of each serovar grown in the two different hosts were detected by two-dimensional (2-D) gel electrophoresis and fluorography of radioactively labelled proteins. At least four to six serial passages in the alternative host were necessary before the changes were apparent. Iodination of suspensions of purified chlamydiae and 2-D electrophoresis revealed several surface proteins that were determined by the host cells in which the bacteria had replicated. These iodinated chlamydial proteins were removed by treatment of the iodinated EB with trypsin, indicating their location at the bacterial surface. Two of the major constituents of the outer-membrane complex, the cysteine- and methionine-rich 60 kDa and 40 kDa proteins, remained unchanged in both molecular mass and charge during the host adaptation. Several chlamydial proteins capable of binding iodinated host membrane preparations also exhibited host-dependent alterations. Immunoblotting experiments with a rabbit and a human polyclonal sera indicated that distinct host-specified chlamydial proteins were reactive with the two sera.
authors: Department of Microbiology, St Louis University School of Medicine, MO 63104-1028.
authors: P C
authors: PC
authors: Goswami
authors: E
authors: E
authors: Vretou
authors: S K
authors: SK
authors: Bose
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics*; D015153:Blotting, Western; D002692:Chlamydia trachomatis / Q000235:genetics* / Q000254:growth & development; D014644:Genetic Variation; D006367:HeLa Cells; D006801:Humans; D008715:Methionine / Q000378:metabolism; D011817:Rabbits
chemical_list: D001426:Bacterial Proteins; D008715:Methionine