title: [The diagnostic effectiveness of an immunoenzyme test system for determining diphtheria toxin antigens in the blood serum in a clinical laboratory trial].
abstract: The data on the approbation of the diagnostic value of the enzyme immunoassay (EIA) system for the determination of diphtheria toxin in the blood sera of diphtheria patients and persons suspected for diphtheria are presented. The EIA system was prepared on the basis of F(ab)2 fractions of purified antidiphtheria antibodies. 240 serum samples from diphtheria and tonsillitis patients and from healthy persons were studied. Diphtheria toxin was determined in all patients with the toxic form of diphtheria and in 41.3% of patients with its localized forms. Blood was taken mainly of the first week of the disease. In healthy persons the results of EIA were negative. Thus, the trial of the assay system in a clinical laboratory showed its good diagnostic effectiveness. The use of this EIA system in medical practice is believed to be quite promising.
authors: N M
authors: NM
authors: Nikitiuk
authors: L Iu
authors: LIu
authors: Smurova
authors: T S
authors: TS
authors: Shobukhova
authors: T A
authors: TA
authors: Zykova
authors: I N
authors: IN
authors: Filimonova
authors: E B
authors: EB
authors: Ershova
mesh_terms: D000293:Adolescent; D000328:Adult; D000942:Antigens, Bacterial / Q000097:blood*; D001782:Blood Donors; D002353:Carrier State / Q000175:diagnosis; D004165:Diphtheria / Q000175:diagnosis*; D004167:Diphtheria Toxin / Q000276:immunology*; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D008297:Male; D008875:Middle Aged; D011933:Reagent Kits, Diagnostic; D014586:USSR
chemical_list: D000942:Antigens, Bacterial; D004167:Diphtheria Toxin; D011933:Reagent Kits, Diagnostic
vernacular_title: Diagnosticheskaia éffektivnost' immunofermentnoĭ test-sistemy dlia opredeleniia antigenov difteriĭnogo toksina v syvorotke krovi pri kliniko-laboratornym ispytanii.