A novel polyclonal antibody (CL-B1/29) for immunolocalization of transforming growth factor-beta 2 (TGF-beta 2) in adult mouse.
A polyclonal antibody (CL-B1/29) raised against a synthetic peptide with an amino acid sequence identical to the first 29 N-terminal residues of bovine bone-derived transforming growth factor-beta 2 (TGF-beta 2) was characterized and used for immunolocalization of TGF-beta 2 in adult mice. Reduced staining of immunoblots and tissue after absorption of the antiserum with the immunizing peptide or with TGF-beta 2 but not with purified TGF-beta 1 demonstrated that the reagent is specific for TGF-beta 2, with little or no crossreactivity with TGF-beta 1. The immunolocalization of TGF-beta 2 was investigated in formalin-fixed, paraffin-embedded cultured cells and murine tissue. Specimens pre-digested with testicular hyaluronidase demonstrated immunostaining predominantly of extracellular connective tissue matrix, whereas specimens pre-digested with pronase E demonstrated primarily cytoplasmic staining. Immunoreactivity was widely distributed in connective tissue, muscle, adsorptive and secretory epithelia, especially of endocrine tissue, and neural tissue of adult mice.
Ksander
G A
GA
Department of Histology, Celtrix Laboratories, Palo Alto, California 94303.
Gerhardt
C O
CO
Dasch
J R
JR
Ellingsworth
L R
LR
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D003238:Connective Tissue / Q000737:chemistry; D004702:Endocrine Glands / Q000737:chemistry; D004848:Epithelium / Q000737:chemistry; D005109:Extracellular Matrix / Q000737:chemistry; D006821:Hyaluronoglucosaminidase; D051379:Mice; D009132:Muscles / Q000737:chemistry; D009420:Nervous System / Q000737:chemistry; D011402:Pronase; D016212:Transforming Growth Factor beta / Q000032:analysis* / Q000276:immunology
D000906:Antibodies; D016212:Transforming Growth Factor beta; D006821:Hyaluronoglucosaminidase; D011402:Pronase