Differentiation of Achromobacter-like strains from human blood by DNA restriction endonuclease digest and ribosomal RNA gene probe patterns.
Variation amongst Achromobacter-like strains was examined by DNA restriction endonuclease digestion and rDNA gene patterns generated using a non-radioactive probe. Chromosomal DNA was extracted from 12 cultures representing Achromobacter groups B, E and F, all from human blood cultures. DNA fingerprinting using EcoRI, Hae III or HindIII sub-divided the strains in a similar manner to that obtained by their protein patterns. The HaeIII patterns, with their small number of bands, were the easiest to interpret. The EcoRI patterns included a species-species triplet of bands but minor band patterns allowed further differentiation. The Achromobacter group F strains comprised a separate taxon and were distinct from the group B and E strains by all techniques examined. The study demonstrates that, in addition to total DNA digest analysis, rDNA gene restriction patterns provide a simple but discriminatory electrophoretic method for distinguishing within Achromobacter groups B and E.
Holmes
B
B
National Collection of Type Cultures, Central Public Health Laboratory, London, England.
Costas
M
M
Wood
A C
AC
Owen
R J
RJ
Morgan
D D
DD
D000421:Alcaligenes / Q000145:classification / Q000235:genetics*; D015139:Blotting, Southern; D016172:DNA Fingerprinting; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D004275:DNA, Ribosomal / Q000032:analysis*; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D014644:Genetic Variation; D006801:Humans; D009693:Nucleic Acid Hybridization; D012335:RNA, Ribosomal / Q000235:genetics; D015183:Restriction Mapping; D018805:Sepsis / Q000382:microbiology*
D015342:DNA Probes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases