Isoprenoid metabolism in Plasmodium falciparum during the intraerythrocytic phase of malaria.
Products of the isoprenoid metabolism were identified upon incubations of extracts from Plasmodium falciparum infected red blood cells with [14C] mevalonate. Uninfected erythrocytes and wild type yeast Saccharomyces cerevisiae extracts were used as controls. In parasitized red blood cells as well as in yeast extracts, mevalonate was converted into the biosynthetic isoprenoid precursors of sterol pathway until farnesyl pyrophosphate. In contrast, no mevalonate conversion was observed in uninfected erythrocyte extracts. The isoprenoid metabolism appeared stage-dependent as shown by the increase of radiolabelled farnesyl pyrophosphate amount at the beginning of the schizogonic phase (30-36 hours).
Mbaya
B
B
Laboratoire de Biologie Cellulaire, URA CNRS n. 290, Poitiers, France.
Rigomier
D
D
Edorh
G G
GG
Karst
F
F
Schrevel
J
J
D000085:Acetates / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D004912:Erythrocytes / Q000469:parasitology*; D045782:Hemiterpenes; D006801:Humans; D008288:Malaria / Q000378:metabolism*; D008798:Mevalonic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism*; D010963:Plasmodium falciparum / Q000378:metabolism*; D011106:Polyisoprenyl Phosphates / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D012717:Sesquiterpenes
D000085:Acetates; D045782:Hemiterpenes; D009943:Organophosphorus Compounds; D011106:Polyisoprenyl Phosphates; D012717:Sesquiterpenes; C045038:phosphomevalonic acid; C004809:isopentenyl pyrophosphate; C004808:farnesyl pyrophosphate; D008798:Mevalonic Acid