Counterproductive transcriptional and translational regulation of elongation factor 1-alpha synthesis during early development in sea urchins.
We have isolated a cDNA clone encoding elongation factor 1-alpha (EF1-alpha) and used probes prepared from this cDNA to measure levels of EF1-alpha transcripts during early development. We also determined the fraction of EF1-alpha transcripts in polysomes during this time period. Following the blastula stage there is a sharp increase in the amount of EF1-alpha mRNA. This pattern of accumulation is similar to other previously described sea urchin mRNAs. However, while the level of EF1-alpha mRNA is increasing, the fraction of EF1-alpha mRNA in polysomes decreases. Thus, there is an apparently counter-productive decrease in efficiency of recruitment into polysomes occurring concurrently with an increase in the overall amount of EF1-alpha mRNA.
Peeler
M T
MT
Department of Zoology, University of Texas, Austin 78712.
Kelso-Winemiller
L
L
Wu
M F
MF
Skipper
J K
JK
Winkler
M M
MM
D000818:Animals; D005786:Gene Expression Regulation; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000096:biosynthesis / Q000235:genetics; D012270:Ribosomes / Q000378:metabolism; D012617:Sea Urchins / Q000196:embryology* / Q000235:genetics; D014981:Xenopus
D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D012333:RNA, Messenger; D012261:Ribonucleoproteins