Mapping of the microvillar 110K-calmodulin complex (brush border myosin I). Identification of fragments containing the catalytic and F-actin-binding sites and demonstration of a calcium ion dependent conformational change.
In intestinal microvilli, the 110K-calmodulin complex is the major component of the cross-bridges which connect the core bundle of actin filaments to the membrane. Our previous work showed that the 110-kDa polypeptide can be divided into three functional domains: a 78-kDa fragment that contains the ATPase activity and the ATP-reversible F-actin-binding site, a 12-kDa fragment required for binding calmodulin molecules, and a terminal 20-kDa domain of unknown function [Coluccio, L. M., & Bretscher, A. (1988) J. Cell Biol. 106, 367-374]. By analysis of limited alpha-chymotryptic cleavage products, we now show that the molecular organization is very similar to that described for the S1 fragment of myosin. The catalytic site was identified by photoaffinity labeling with [5,6-3H]UTP, and fragments binding F-actin were identified by cosedimentation assays. Cleavage of the 78-kDa fragment yielded major fragments of 32 and 45 kDa, followed by cleavage of the 45-kDa fragment to a 40-kDa fragment. Of these, only the 32-kDa fragment was labeled by [5,6-3H]UTP. Physical characterization revealed that the 45- and 32-kDa fragments exist as a complex that can bind F-actin, whereas the 40-kDa/32-kDa complex cannot bind actin. We conclude that the catalytic site is located in the 32-kDa fragment and the F-actin-binding site is present in the 45-kDa fragment; the ability to bind actin is lost upon further cleavage of the 45-kDa fragment to 40 kDa. Peptide sequence analysis revealed that the 45-kDa fragment lies within the molecule and suggests that the 32-kDa fragment is the amino terminus.(ABSTRACT TRUNCATED AT 250 WORDS)
Coluccio
L M
LM
Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322.
Bretscher
A
A
D000199:Actins / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D002147:Calmodulin / Q000302:isolation & purification / Q000378:metabolism*; D002148:Calmodulin-Binding Proteins; D002645:Chickens; D002918:Chymotrypsin; D004591:Electrophoresis, Polyacrylamide Gel; D007413:Intestinal Mucosa / Q000378:metabolism; D046911:Macromolecular Substances; D008871:Microvilli / Q000378:metabolism*; D008970:Molecular Weight; D009132:Muscles / Q000378:metabolism; D018995:Myosin Heavy Chains; D024461:Myosin Type I; D009218:Myosins / Q000302:isolation & purification / Q000378:metabolism*; D010449:Peptide Mapping; D011487:Protein Conformation; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D011817:Rabbits; D014544:Uridine Triphosphate / Q000378:metabolism
D000199:Actins; D002147:Calmodulin; D002148:Calmodulin-Binding Proteins; C062291:MYO1A protein, human; D046911:Macromolecular Substances; D011506:Proteins; D002918:Chymotrypsin; D024461:Myosin Type I; D018995:Myosin Heavy Chains; D009218:Myosins; D002118:Calcium; D014544:Uridine Triphosphate