title: Simultaneous synthesis of multiple oligonucleotides using nucleoside-H-phosphonate intermediates.
abstract: A semimechanized simultaneous synthesis of multiple oligonucleotides using nucleoside-H-phosphonates as intermediates was developed and applied to the preparation of hybridization probes. The synthesis could be done with equivalent results (about 98% average yield per cycle) using regenerated H-phosphonates.
authors: Universität Ulm, Sektion Polymere, Oberer Eselsberg, FRG.
authors: H
authors: H
authors: Seliger
authors: R
authors: R
authors: Rösch
mesh_terms: D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D005821:Genetic Techniques; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000138:chemical synthesis; D009841:Oligonucleotides / Q000138:chemical synthesis*; D063065:Organophosphonates / Q000737:chemistry
chemical_list: D015345:Oligonucleotide Probes; D009841:Oligonucleotides; D063065:Organophosphonates