Functional and immunogenetic characterization of FcR-blocking antibody.
The characteristics and functional importance of FcR-blocking antibodies and their production were investigated after immunization with whole blood, "buffy coat" and purified platelets. We studied the presence of FcR-blocking antibody in haemodialyzed, transfused patients waiting for kidney transplantation, and we found strong correlation between the blocking effect and better graft survival. We suggest that this blocking antibody does not attack FcR as primary target. Investigation of blocking activity of ten different immune sera on 50 healthy panel cells showed that target antigen has some polymorphic varieties. On basis of family studies it seems that the target antigen is not linked to HLA haplotype. The blocking effect of sera could be removed by absorption of CD8+ cells, B lymphocytes, platelets and granulocytes, but not with erythrocytes, monocytes, CD8- cells and NK cells.
Padányi
A
A
National Institute of Haematology and Blood Transfusion, Budapest, Hungary.
Gyódi
E
E
Sarmay
G
G
Réti
M
M
Mayer
K
K
Kassai
M
M
Petrányi
G G
GG
D000042:Absorption; D000906:Antibodies / Q000276:immunology*; D001792:Blood Platelets / Q000276:immunology; D001803:Blood Transfusion; D002469:Cell Separation; D005260:Female; D005838:Genotype; D006085:Graft Survival / Q000276:immunology; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D007114:Immunization; D007132:Immunoglobulin Isotypes / Q000276:immunology; D007165:Immunosuppression Therapy; D007519:Isoantigens / Q000276:immunology; D016030:Kidney Transplantation / Q000276:immunology; D017710:Lymphocyte Transfusion; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D017713:Platelet Transfusion; D011961:Receptors, Fc / Q000276:immunology*; D006435:Renal Dialysis
D000906:Antibodies; D006680:HLA Antigens; D007132:Immunoglobulin Isotypes; D007519:Isoantigens; D011961:Receptors, Fc