Characteristics of mouse to rat xenograft heart transplantation.
A simple technique for grafting a mouse heart heterotopically to rat neck vessels in experimental xenotransplantation is described. In this series, graft survival was tested with the following modes of immunomodulation: ciclosporin (Cy), athymic and nude rats, splenectomy, total lymphoid irradiation (TLI), 15-deoxyspergualin in two different doses and various combinations of these. The mean graft survival time, with Cy or athymic and nude rats as recipients, was 3 days as also seen in animals without treatment. With either irradiation, splenectomy or 15-deoxyspergualin, 10 mg/kg daily, the graft survival time was prolonged to 7.8, 4.7 and 6.8 days, respectively. In combination, TLI + splenectomy gave a graft survival of 9.7 days and Cy + splenectomy 7.2 days. With 15-deoxyspergualin at 10 mg/kg/day plus splenectomy, done 1 week prior to transplantation, graft survival was extended to 17.8 days.
Gannedahl
G
G
Department of Urology, University of Uppsala, Sweden.
Fellström
B
B
Larsson
E
E
Roos-Engstrand
E
E
Tufveson
G
G
D000818:Animals; D000917:Antibody Formation; D003524:Cyclosporins / Q000494:pharmacology; D005260:Female; D006085:Graft Survival / Q000187:drug effects; D006146:Guanidines / Q000494:pharmacology; D016027:Heart Transplantation / Q000401:mortality; D007166:Immunosuppressive Agents / Q000494:pharmacology; D015182:Lymphatic Irradiation; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D051381:Rats; D011919:Rats, Inbred Strains; D014183:Transplantation, Heterologous
D003524:Cyclosporins; D006146:Guanidines; D007166:Immunosuppressive Agents; C037258:gusperimus