Biological cryosection preparation and practical ion yield evaluation for ion microscopic analysis.
A mounting method utilizing an indium substrate is described for preparing freeze-dried cryosections of biological tissue for ion microscopic analysis. Using this procedure, a qualitative comparison between cryosection, conventional chemical, and freeze-substitution specimen preparations is made with rat liver tissue. Practical ion yield variations in cryosections are found to be minimal (+/- 13% relative standard deviation) in tissue-containing areas of rat liver and small intestine.
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Department of Chemistry, Cornell University, Ithaca, NY 14853.
Crooker
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Morrison
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D000818:Animals; D004577:Electron Probe Microanalysis; D005612:Freeze Drying; D005629:Frozen Sections; D007091:Image Processing, Computer-Assisted; D007421:Intestine, Small / Q000648:ultrastructure*; D008099:Liver / Q000648:ultrastructure*; D013058:Mass Spectrometry; D051381:Rats