A conserved cysteine in molybdenum oxotransferases.
The amino acid sequences of peptides derived from rat hepatic sulfite oxidase have been determined by a combination of amino acid analysis and Edman degradation of the purified protein. The data obtained showed the rat liver enzyme contained 3 cysteine residues which was confirmed by thiol modification studies using 4,4'-dithiodipyridine of the native enzyme. Combining these data with that previously published for chicken liver sulfite oxidase (Neame, P. J., and Barber, M. J. (1989) J. Biol. Chem. 264, 20894-20901) indicates that 2 cysteines (Cys186 and Cys430, based upon the numbering for the chicken sequence) are conserved in both chicken and rat liver enzymes with all the cysteine residues being present in the molybdenum-containing domain. Further comparison of the sequences of the molybdenum domains of rat and chicken liver sulfite oxidase with the amino acid sequences published for the molybdenum domains of a variety of assimilatory nitrate reductases suggests that only a single cysteine residue (Cys186) is conserved in all these enzymes, indicating that it may play a role in the binding of Mo-pterin to the protein.
Barber
M J
MJ
Department of Biochemistry and Molecular Biology, University of South Florida, College of Medicine, Tampa.
Neame
P J
PJ
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002645:Chickens; D003545:Cysteine; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D008982:Molybdenum; D050897:Nitrate Reductase (NADPH); D009565:Nitrate Reductases / Q000235:genetics*; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000235:genetics*; D010446:Peptide Fragments / Q000302:isolation & purification; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013438:Sulfhydryl Compounds / Q000032:analysis
D010446:Peptide Fragments; D013438:Sulfhydryl Compounds; D008982:Molybdenum; D009565:Nitrate Reductases; D050897:Nitrate Reductase (NADPH); D050862:Oxidoreductases Acting on Sulfur Group Donors; D003545:Cysteine