title: Inhibition of EGF-induced phospholipase C activation in A431 cells by erbstatin, a tyrosine kinase inhibitor.
abstract: Erbstatin, a tyrosine kinase inhibitor, inhibited epidermal growth factor (EGF)-induced inositol phosphate production in cultured A431 cells. However, it did not inhibit ATP-induced inositol phosphate production. Cytosolic but not membrane-associated phospholipase C was activated by EGF, and erbstatin inhibited enhancement of the phospholipase C activity in EGF-treated cells. Thus, tyrosine kinase of A431 cells is suggested to be functionally involved in phospholipase C activation.
authors: Department of Applied Chemistry, Faculty of Science and Technology, Keio University, Yokohama, Japan.
authors: M
authors: M
authors: Imoto
authors: N
authors: N
authors: Shimura
authors: H
authors: H
authors: Ui
authors: K
authors: K
authors: Umezawa
mesh_terms: D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D004789:Enzyme Activation; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D006801:Humans; D006873:Hydroquinones / Q000494:pharmacology*; D007294:Inositol / Q000378:metabolism; D007295:Inositol Phosphates / Q000378:metabolism; D007700:Kinetics; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors*; D010738:Type C Phospholipases / Q000378:metabolism*
chemical_list: D006873:Hydroquinones; D007295:Inositol Phosphates; D007294:Inositol; D004815:Epidermal Growth Factor; D011505:Protein-Tyrosine Kinases; D010738:Type C Phospholipases; C048212:erbstatin