[Inhibition of glucose in Zajdela hepatoma by 6-phosphogluconate; the role of 3-phosphoglycerate].
Influence of 6-phosphogluconate and 3-phosphoglycerate have been studied for their effect on the fructose-6-phosphate glycolytic transformation reactions in homogenates of the Zajdela hepatoma cells. It is established that 6-phosphogluconate inhibits formation of lactate from fructose-6-phosphate and increases the ratio: dioxyacetone-phosphate/lactate. The influence of 6-phosphogluconate on the formation of lactate from the fructose-1,6-bisphosphate is similar. 3-phosphoglycerate removes the effect of 6-phosphogluconate, its content being unchanged in samples, which indicates rather the regulatory, than the substrate role of 3-phosphoglycerate. Analogous experiments with homogenates of the rat liver show that 6-phosphogluconate inhibits hexosephosphate isomerase, but almost all the introduced substrate (fructose-6-phosphate) is transformed into glucose. Processes of fructose-6-phosphate consumption in the hepatoma and liver are opposite.
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D000818:Animals; D005636:Fructosephosphates / Q000378:metabolism; D005942:Gluconates / Q000494:pharmacology*; D005988:Glyceric Acids / Q000494:pharmacology*; D006019:Glycolysis / Q000187:drug effects*; D007773:Lactates / Q000096:biosynthesis; D019344:Lactic Acid; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D051381:Rats
D005636:Fructosephosphates; D005942:Gluconates; D005988:Glyceric Acids; D007773:Lactates; D019344:Lactic Acid; C027618:fructose-6-phosphate; C005156:3-phosphoglycerate; C008884:6-phosphogluconic acid
Ingibuvaniia glikolizy v gepatomi Zaĭdela 6-fosfogliukonatom; rol' 3- fosfoglitseratu.