An in vivo microdialysis characterization of extracellular dopamine and GABA in dorsolateral striatum of awake freely moving and halothane anaesthetised rats.
This study describes the results of a systematic characterization of extracellular dopamine (DA) and gamma-aminobutyric acid (GABA) recovered from dorsolateral striatum using in vivo microdialysis in rats following acute (2.5 h) and chronic (1 day, 2 day and 4 day) implantation of the probe. The voltage and calcium dependence of DA and GABA overflow was characterised by perfusion with the sodium channel blocker tetrodotoxin (TTX 10-6M) and with Ca2(+)-free Ringers perfusion medium. In addition, the effect of halothane anaesthesia on the responsiveness of these neurotransmitter substances to TTX and Ca2(+)-free perfusion medium was investigated. Perfusion with TTX decreased basal DA levels by at least 60% in all groups. The TTX-induced decrease was most profound in halothane-anaesthetised rats, 24 h after implantation of the probe. Responsiveness of GABA to TTX infusion was different between the groups. In acutely implanted halothane-anaesthetised rats basal GABA levels were unaltered by perfusion with TTX while in the remaining groups at least a 35% reduction was observed. In awake rats 2 days following implantation of the probe removal and replacement of the Ca2+ from the perfusion medium resulted in a reversible reduction of basal DA by 87%. In addition, basal GABA levels were decreased by 52%. This decrease was delayed and was not reversed 1.5 h after the Ca2(+)-free perfusion medium was replaced with normal perfusion medium although basal GABA levels returned to pre-experimental levels by the following day.(ABSTRACT TRUNCATED AT 250 WORDS)
Osborne
P G
PG
Department of Pharmacology, Karolinska Institute, Stockholm, Sweden.
O'Connor
W T
WT
Drew
K L
KL
Ungerstedt
U
U
D000758:Anesthesia; D000818:Animals; D002118:Calcium / Q000502:physiology; D003342:Corpus Striatum / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D003956:Dialysis; D004298:Dopamine / Q000737:chemistry / Q000378:metabolism*; D006221:Halothane; D008297:Male; D008832:Microchemistry; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D013779:Tetrodotoxin / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000737:chemistry / Q000378:metabolism*
D013779:Tetrodotoxin; D005680:gamma-Aminobutyric Acid; D002118:Calcium; D006221:Halothane; D004298:Dopamine