Automated flow cytometric analysis of drug susceptibility of malaria parasites.
A method is described for the fully automated reading of Plasmodium falciparum drug susceptibility tests. Cultured material was fixed and could be stored for greater than or equal to 6 months until analysis. The parasites were stained for DNA with the fluorescent dye Hoechst 33258 and analyzed by flow cytometry. The procedure was done in 96-well microtiter plates, after which the material was directed through the sensing region in the flow cytometer. The data resulting from the analysis were stored by microcomputer and processed by a program developed for this purpose. Using this method, a number of different parameters describing the growth in culture can be assessed.
van Vianen
P H
PH
University of Leiden, The Netherlands.
Thaithong
S
S
Reinders
P P
PP
van Engen
A
A
van der Keur
M
M
Tanke
H J
HJ
van der Kaay
H J
HJ
Mons
B
B
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D002478:Cells, Cultured; D016054:DNA, Protozoan / Q000032:analysis; D004351:Drug Resistance; D001330:Electronic Data Processing; D004912:Erythrocytes / Q000469:parasitology; D005434:Flow Cytometry; D008838:Microcomputers; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000254:growth & development
D000962:Antimalarials; D016054:DNA, Protozoan