Use of a cell hybrid test system to demonstrate that benomyl induces aneuploidy and polyploidy.
We have monitored the segregation of a single human chromosome in a human-Chinese hamster hybrid cell line, EUBI, following exposure to benomyl. We found a dose-dependent increase in frequency of aneuploidy, but a much more marked induction of polyploidy was noted at the highest benomyl concentration. We confirm the usefulness of this assay for determining genetic risk associated with human exposure to environmental chemicals.
Zelesco
P A
PA
Department of Genetics and Human Variation, La Trobe University, Bundoora, Australia.
Barbieri
I
I
Graves
J A
JA
D000782:Aneuploidy; D000818:Animals; D001542:Benomyl / Q000633:toxicity*; D002460:Cell Line; D002875:Chromosomes / Q000187:drug effects*; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D006822:Hybrid Cells / Q000187:drug effects*; D007621:Karyotyping; D009152:Mutagenicity Tests / Q000379:methods*; D011123:Polyploidy
D001542:Benomyl