The prevalence of human immunodeficiency virus in patients and their spouses entering a large in vitro fertilization program.
Between September 1987 and August 1989, all patients and their spouses entering our in vitro fertilization (IVF) program were screened for the human immunodeficiency virus (HIV) using the enzyme-linked immunosorbent assay (ELISA). Of 848 patients and 848 spouses tested, all but 4 patients and 1 husband tested negative. Of those who tested positive on repeat testing with ELISA, only one was positive on Western blotting (HIV prevalence, 0.59 per 1000). During this same time period 1187 samples of human cord blood were used to make tissue culture medium for the IVF embryology laboratory. One sample was discarded because of positive HIV on ELISA and Western blotting; two other samples were discarded because of positivity to the hepatitis B surface antigen. While we believe that routine HIV screening of IVF patients and their spouses is indicated, this population is of low risk for HIV positivity. Furthermore, continued screening of human sera used to make tissue culture media for IVF is mandatory.
Edelstein
M C
MC
Department of Obstetrics and Gynecology, Eastern Virginia Medical School, Norfolk 23507.
Kruithoff
C
C
Brothman
L
L
Veeck
L L
LL
Alexander
N J
NJ
Muasher
S J
SJ
D015153:Blotting, Western; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D005260:Female; D005307:Fertilization in Vitro; D005312:Fetal Blood / Q000382:microbiology; D015483:HIV Antibodies / Q000097:blood; D015528:HIV Seroprevalence; D006801:Humans; D008297:Male; D014481:United States / Q000453:epidemiology
D003470:Culture Media; D015483:HIV Antibodies