Relative effectiveness of yeast cell wall digesting enzymes on Yarrowia lipolytica.
Novozym 234 at a concentration of 1.0 mg/ml yielded 95.5% spheroplasts within 30 min at 37 degrees C, pH 7.0, with 36% regeneration which was the highest level of regeneration observed. Yeast lytic enzyme at a concentration of 1.0 mg/ml yielded 99.8% spheroplasts with only 1.5% regeneration. Glusulase was significantly less active in producing osmotically sensitive cells. All three enzymes yielded significantly higher levels of osmotically sensitive cells when cells were harvested from the mid-logarithmic phase of growth compared with later growth phases. beta-Glucuronidase failed to produce osmotically sensitive cells regardless of the phase of growth from which cells were harvested.
Lin
L F
LF
Department of Food Science, University of Massachusetts, Amherst 01003.
Levin
R E
RE
D002473:Cell Wall / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism; D009097:Multienzyme Complexes / Q000378:metabolism; D009994:Osmolar Concentration; D012440:Saccharomyces / Q000201:enzymology*; D004718:Saccharomycetales / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D013104:Spheroplasts / Q000502:physiology; D013429:Sulfatases / Q000378:metabolism
D004798:Enzymes; D009097:Multienzyme Complexes; C023059:glusulase; C045154:novozym 234; D013429:Sulfatases; D005966:Glucuronidase