title: A family of homologous X chromosomal sequences with some members showing evidence of enhancer activity.
abstract: Little is known about either the frequency of occurrence of enhancers or the interrelationships of various enhancers in mammalian genomes. Previous reports described a potent, transcriptional enhancer within the sequence termed "Xrep," a human X chromosomal genomic clone (Riley et al., 1986; Riley, 1989a). Additional Xrep-related sequences reside on the human X chromosome. A probe for the Xrep enhancer hybridized with multiple restriction fragments located on the X chromosome. Two of the fragments cross-hybridizing with the enhancer had identical enhancer-like sequences but dissimilar flanking sequences. However, both of the sequenced enhancers were flanked by poly(GT) repeats strongly implicated in the generation of polymorphisms. A third sequence cross-hybridizing to the enhancer probe was found, in chromatin form, to be extremely DNase I hypersensitive.
authors: Department of Urology, University of Washington, Seattle 98195.
authors: D E
authors: DE
authors: Riley
mesh_terms: D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D006224:Cricetinae; D004587:Electrophoresis, Agar Gel; D004742:Enhancer Elements, Genetic; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014960:X Chromosome