[Histologic grade, DNA content and image analysis in soft tissue tumors].
Flow cytometry and image analysis technique were used to quantitate the nuclei of various soft tissue tumors. A single representing section from soft tissue sarcoma was used for histologic grading. Histologic and cytometric comparative analyses showed that all 21 benign tumors were diploid. Among 62 cases of soft tissue sarcoma, 45 (73%) were aneuploid. There was a significant difference in the nuclear area between benign and malignant tumors (P less than 0.01), diploid and aneuploid tumors (P less than 0.05). The two new techniques are valuable in cellular quantitative measurement for soft tissue tumors.
Shi
Y Q
YQ
Cancer Hospital, Shanghai Medical College.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000782:Aneuploidy; D002467:Cell Nucleus; D002648:Child; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D008875:Middle Aged; D012509:Sarcoma / Q000235:genetics / Q000473:pathology*; D012983:Soft Tissue Neoplasms / Q000235:genetics / Q000473:pathology*
D004273:DNA, Neoplasm