Occurrence and structure of lipoteichoic acids in the genus Staphylococcus.
Lipoteichoic acids were isolated from eleven species of the genus Staphylococcus using phenol-water partition and hydrophobic chromatography on octyl-Sepharose CL-4B. The lipoteichoic acids purified could be visualized by SDS-PAGE. They were shown to be composed of a hydrophilic poly(glycerophosphate) chain covalently linked to gentiobiosyldiacylglycerol, the common lipid anchor of these molecules. Glycerophosphate units of the hydrophilic chain were found to be partly substituted with ester-linked D-alanine, except in the case of S. cohnii. The lipoteichoic acids isolated from S. cohnii, S. hominis, S. saprophyticus and S. simulans contain alpha(1-2)-linked N-acetylglucosamine as an additional substituent of the poly(glycerophosphate) backbone.
Ruhland
G J
GJ
Institut für Genetik und Mikrobiologie, Universität München, Federal Republic of Germany.
Fiedler
F
F
D002849:Chromatography, Gas; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D004591:Electrophoresis, Polyacrylamide Gel; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D015394:Molecular Structure; D013210:Staphylococcus / Q000032:analysis*; D013682:Teichoic Acids / Q000737:chemistry* / Q000302:isolation & purification
D008070:Lipopolysaccharides; D013682:Teichoic Acids; C009900:lipoteichoic acid