Induction of morphological transformation by coal-dust extract in BALB/3T3 A31-1-13 cell line.
The transforming activity of coal dust extracts was studied using BALB/3T3 clone A31-1-13 cells. Coal-dust extracts, both nitrosated and nonnitrosated, induced cell transformation in a dose-response manner. However, the transformation frequency was higher with the nitrosated than with the nonnitrosated extract. All transformed cell lines derived from coal-dust extract-induced foci showed biological characteristics of neoplastic transformation such as loss of contact inhibition and anchorage-independent growth. These results appear to support a hypothesis of coal mine dust causation of gastric cancer in coal miners.
Wu
Z L
ZL
Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Morgantown, WV 26505-2888.
Chen
J K
JK
Ong
T
T
Matthews
E J
EJ
Whong
W Z
WZ
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002461:Cell Line, Transformed; D002470:Cell Survival; D002471:Cell Transformation, Neoplastic; D003031:Coal / Q000633:toxicity*; D004391:Dust; D051379:Mice; D015538:Nitrosation
D003031:Coal; D004391:Dust