The effect of bovine lactoferrin on muscle growth in vivo and in vitro.
Lactoferrin was found to be a potent stimulator of proliferation for L6 myoblasts. Both apo and holo-forms of lactoferrin were equipotent. By contrast, only the holo-form of transferrin (a structurally related iron binding protein) stimulated proliferation, apo-transferrin was without activity. Holo-transferrin was also less stimulatory than lactoferrin. Purified lactoferrin was administered to mature female rats and to neonatal rats by daily subcutaneous injection to determine if there was a measurable effect on muscle cell growth in vivo. Results from the in vivo studies suggest that lactoferrin has little or no effect on muscle cell growth in the whole animal.
Byatt
J C
JC
Monsanto Company, St. Louis, Missouri 63198.
Schmuke
J J
JJ
Comens
P G
PG
Johnson
D A
DA
Collier
R J
RJ
D000818:Animals; D000831:Animals, Newborn; D002417:Cattle; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005260:Female; D007781:Lactoferrin / Q000008:administration & dosage / Q000494:pharmacology*; D008934:Mitogens / Q000008:administration & dosage / Q000494:pharmacology*; D024510:Muscle Development; D009132:Muscles / Q000166:cytology / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains
D008934:Mitogens; D007781:Lactoferrin