Particle counting immunoassay of choriogonadotropin using monoclonal antibodies.
A latex particle immunoassay has been developed for the quantification of choriogonadotropin in human serum using two monoclonal antibodies specific for the beta-chain of the hormone. The assay, based on optical counting of monomeric particles, was achieved in 40 min and the calibration curve was linear between 10 and 200 IU/l. Intra- and interassay precisions at three different levels of the curve varied between 3.3 and 10.9%. The method was validated by comparison with two different radioimmunoassays and correlation coefficients of 0.97-0.99 were obtained.
Halflants
M
M
Unit of Experimental Medicine, Faculty of Medicine, Catholic University of Louvain, Brussels, Belgium.
Tasiaux
N
N
Van Krieken
L
L
De Hertogh
R
R
Collet-Cassart
D
D
D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002138:Calibration; D006063:Chorionic Gonadotropin / Q000097:blood*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007841:Latex Fixation Tests / Q000379:methods; D010434:Pepsin A; D015203:Reproducibility of Results
D000911:Antibodies, Monoclonal; D006063:Chorionic Gonadotropin; D010434:Pepsin A