The effect of selenium ions on the catabolism of prostaglandin E2 in rabbit kidney cortex.
The effect of selenium ions on the catabolism of prostaglandin E2 by 15-hydroxy prostaglandin dehydrogenase and prostaglandin delta 13 reductase in rabbit kidney cortex was examined. Selenium ions had no effect on the 15-hydroxy prostaglandin dehydrogenase activity. On the other hand, selenium ions inhibited the prostaglandin delta 13 reductase activity at concentrations ranging from 0.05 to 0.5 mM. The effect was concentration-dependent. The inhibition by selenium ions was non-competitive with regard to NADH and 15-keto PGE2, respectively. These results suggest that selenium ions have the potential to increase 15-keto PG levels in the kidney by inhibiting the prostaglandin delta 13 reductase activity and that such action of selenium ions may participate in renal damage of selenium poisoning.
Sakuma
S
S
Department of Hygienic Chemistry, Osaka University of Pharmaceutical Sciences, Japan.
Fujimoto
Y
Y
Hikita
E
E
Okano
Y
Y
Yamamoto
I
I
Fujita
T
T
D000818:Animals; D015232:Dinoprostone / Q000378:metabolism*; D006911:Hydroxyprostaglandin Dehydrogenases / Q000378:metabolism; D007672:Kidney Cortex / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D011817:Rabbits; D012643:Selenium / Q000494:pharmacology*
D006911:Hydroxyprostaglandin Dehydrogenases; C035859:15-hydroxyprostaglandin dehydrogenase; D012643:Selenium; D015232:Dinoprostone