Fetal calf serum increased the zona pellucida penetrability of rat oocytes matured in vitro.
We examined the effect of fetal calf serum (FCS) on meiotic division, subsequent fertilization, and first cleavage to the 2-cell stage of rat oocytes during in vitro maturation. FCS had no effect on the nuclear progression from dictiate to metaphase of the second maturation in vitro and, FCS had no effect on the first cleavage to the 2-cell stage of fertilized oocytes. However, FCS efficiently increased penetration rate of oocytes and shortened the time required for dissolution of the zona pellucida by alpha-chymotrypsin. These results showed that FCS did not affect cytoplasmic maturation necessary for oocytes to develop to the 2-cell stages. We found that FCS only affects the zona pellucida and does not affect the nucleus or cytoplasm of rat oocytes. FCS may prevent hardening of the zona pellucida.
Fujii
Y
Y
Medical Science Laboratory, Center for Adult Diseases, Kurashiki, Japan.
Yoshioka
T
T
Sasaki
J
J
D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000502:physiology; D002970:Cleavage Stage, Ovum / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro; D005312:Fetal Blood / Q000502:physiology*; D066298:In Vitro Techniques; D008540:Meiosis / Q000502:physiology; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D010539:Permeability; D051381:Rats; D015044:Zona Pellucida / Q000502:physiology*