Loss of growth inhibitory activity of TGF-beta toward normal human mammary epithelial cells grown within collagen gel matrix.
TGF-beta at concentrations in the range from 0.1 to 10 ng/ml gave significant growth inhibition of nonmalignant human mammary epithelial cells (HMEC) but not of malignant HMEC grown in monolayer cultures in serum-free medium. However, no growth inhibition of the nonmalignant cells was observed when the cells were cultivated within a type-I collagen gel matrix either adhering to a plastic substratum or floating on the medium. Within floating collagen gels, both nonmalignant and malignant HMEC formed a cell mass having radial extensions, and TGF-beta at 1 or 10 ng/ml prevented the formation of extensions only in the nonmalignant HMEC.
Takahashi
K
K
Department of Biochemistry, Kanagawa Cancer Center, Research Institute, Yokohama, Japan.
Suzuki
K
K
Ono
T
T
D001940:Breast / Q000187:drug effects*; D001943:Breast Neoplasms / Q000473:pathology*; D002448:Cell Adhesion; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D003094:Collagen; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D005782:Gels; D006801:Humans; D010969:Plastics; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D005782:Gels; D010969:Plastics; D016212:Transforming Growth Factor beta; D003094:Collagen