The zinc test as an alternative for acid phosphatase spot tests in the primary identification of seminal traces.
The value of the acid phosphatase spot test in the primary visualization and identification of seminal traces is hampered by the sensitiveness of the enzyme to biodegradation. An alternative spot test is proposed, based on the high concentration of the more stable zinc metal in seminal plasma. The proposed zinc spot test is simple and suitable for on site investigation. Although the sensitivity in fresh stains is lower than that of the acid phosphatase spot test, this is largely compensated by the lower sensitiveness to biodegradation. The specificity for semen is higher than that of the acid phosphatase spot test. In vaginal swabs it was nevertheless seen, that samples should be taken within 24 h after alleged sexual assault to give reliable results.
Hooft
P J
PJ
Katholieke Universiteit Leuven, Laboratory of Forensic Medicine, Belgium.
van de Voorde
H P
HP
D000135:Acid Phosphatase / Q000032:analysis*; D003075:Coitus; D005260:Female; D006801:Humans; D008297:Male; D011237:Predictive Value of Tests; D012661:Semen / Q000737:chemistry* / Q000201:enzymology; D014626:Vaginal Smears; D015032:Zinc / Q000032:analysis*
D000135:Acid Phosphatase; D015032:Zinc