title: Automated flow cytometric analysis of drug susceptibility of malaria parasites.
abstract: A method is described for the fully automated reading of Plasmodium falciparum drug susceptibility tests. Cultured material was fixed and could be stored for greater than or equal to 6 months until analysis. The parasites were stained for DNA with the fluorescent dye Hoechst 33258 and analyzed by flow cytometry. The procedure was done in 96-well microtiter plates, after which the material was directed through the sensing region in the flow cytometer. The data resulting from the analysis were stored by microcomputer and processed by a program developed for this purpose. Using this method, a number of different parameters describing the growth in culture can be assessed.
authors: University of Leiden, The Netherlands.
authors: P H
authors: PH
authors: van Vianen
authors: S
authors: S
authors: Thaithong
authors: P P
authors: PP
authors: Reinders
authors: A
authors: A
authors: van Engen
authors: M
authors: M
authors: van der Keur
authors: H J
authors: HJ
authors: Tanke
authors: H J
authors: HJ
authors: van der Kaay
authors: B
authors: B
authors: Mons
mesh_terms: D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D002478:Cells, Cultured; D016054:DNA, Protozoan / Q000032:analysis; D004351:Drug Resistance; D001330:Electronic Data Processing; D004912:Erythrocytes / Q000469:parasitology; D005434:Flow Cytometry; D008838:Microcomputers; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000254:growth & development
chemical_list: D000962:Antimalarials; D016054:DNA, Protozoan