Estimating age through the amino acid racemization of acid-soluble dentinal peptides.
We studied the possibility of estimating age through the amino acid racemization of acid-soluble dentinal peptide (SP). The correlation of the D/L ratio of aspartic acid and the actual age was the highest in the SP extracted with 1 M HCl (r = 0.996, sigma = +/- 0.8), followed by the total amino acids (TAA:r = 0.995, sigma = +/- 1.0), and the SP extracted with 2 M HCl (r = 0.980, sigma = +/- 1.9) and then SP extracted with 5 M HCl (r = 0.951, sigma = +/- 2.7). The rate of the racemization reaction decreased in the order of SP extracted with 1 M HCl, 2 M HCl, 5 M HCl and the total amino acids (TAA). Consequently, an extraction with 1 M HCl seemed best for estimating the age from SP to serve actual cases.
Ohtani
S
S
Department of Forensic Medicine, Kanagawa Dental College, Japan.
Yamamoto
K
K
D000366:Age Determination by Teeth; D000596:Amino Acids / Q000032:analysis*; D001224:Aspartic Acid / Q000032:analysis; D003804:Dentin / Q000737:chemistry*; D005553:Forensic Dentistry / Q000379:methods; D006801:Humans; D006851:Hydrochloric Acid; D008968:Molecular Conformation; D010455:Peptides / Q000032:analysis*; D012995:Solubility
D000596:Amino Acids; D010455:Peptides; D001224:Aspartic Acid; D006851:Hydrochloric Acid