The Chinese hamster V79 cell mutant V-H4 is phenotypically like Fanconi anemia cells.
It has been shown by genetic complementation analysis that a mitomycin C-sensitive mutant (V-H4) of Chinese hamster V79 cells is the first rodent equivalent of Fanconi anemia (FA) group A. The V-H4 mutant shows many typical characteristics of cells derived from FA patients. V-H4 cells exhibit increased sensitivity towards cross-linking agents as MMC (approximately 30-fold), cis-DDP (approximately 10-fold), DEB (approximately 10-fold), and PUVA (approximately 1.6-fold), but an only slightly increased sensitivity to monofunctional alkylating agents (EMS and MMS) and actinomycin D. V-H4 cells are also moderately sensitive to adriamycin (1.6-fold), and not sensitive to H2O2. The levels of chromosomal aberrations induced by MMC and cis-DDP treatment are higher (4- to 6-fold) in V-H4 cells than in the wild-type V79 cells. Genetic complementation analysis with other Chinese hamster mutants hypersensitive to MMC (irs1, irs1SF, UV20 and UV41) indicates clearly that V-H4 belongs to a different, new complementation group. This unique mutant is very stable and can serve as a vehicle to isolate the complementing FA-A gene from normal human DNA.
Zdzienicka
M Z
MZ
Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, Sylvius Laboratory, The Netherlands.
Arwert
F
F
Neuteboom
I
I
Rooimans
M
M
Simons
J W
JW
D000477:Alkylating Agents; D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D003432:Cross-Linking Reagents; D004249:DNA Damage; D004260:DNA Repair; D005199:Fanconi Anemia / Q000235:genetics*; D005816:Genetic Complementation Test; D006801:Humans; D007364:Intercalating Agents; D010641:Phenotype
D000477:Alkylating Agents; D003432:Cross-Linking Reagents; D007364:Intercalating Agents