The intracellular distribution of cobalt in exposed and unexposed rat myocardium.
The intracellular distribution of cobalt was analysed in the myocardium of exposed and unexposed rats. The exposed rats were given a dietary cobalt supplementation of 40 mg CoSO4.7 H2O/kg body weight for 8 weeks. The mitochondrial fraction showed the greatest relative increase in cobalt: 0.09 ng/mg protein in the unexposed rats to 8.43 ng/mg protein in the exposed rats. In the exposed rats the submitochondrial particles had the highest levels of cobalt: 19.43 ng/mg protein, followed by the sarcoplasmatic reticulum: 12.3 ng/mg protein. The microsomal 44,000 g supernatant also showed an increase, although the levels remained low (0.51 ng/mg protein in the exposed animals). Apparently the calcium-storing organelles had the highest levels of cobalt. This could affect calcium flux in myocardial cells and, secondarily, tension development in cardiac muscle.
Clyne
N
N
Department of Medicine, Karolinska Hospital, Stockholm, Sweden.
Persson
B
B
Havu
N
N
Hultman
E
E
Lins
L E
LE
Pehrsson
S K
SK
Rydström
J
J
Wibom
R
R
D000818:Animals; D003035:Cobalt / Q000493:pharmacokinetics*; D004032:Diet; D006321:Heart / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D008861:Microsomes / Q000378:metabolism; D008929:Mitochondria, Heart / Q000378:metabolism; D009200:Myocardial Contraction / Q000187:drug effects; D009206:Myocardium / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012519:Sarcoplasmic Reticulum / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism
D011506:Proteins; D003035:Cobalt