Control of actin conformation in AML myeloblasts: the effects of bryostatin and TPA.
Protein kinase C (PKC), an enzyme involved in signal transduction, is the receptor for both the tumor-promoting phorbol esters and the anti-neoplastic bryostatins. In many cells, phorbol esters and bryostatins cause similar effects; we have found that both agents increase actin polymerization in neutrophils. In some cells, however, the two agents result in different cell processes; we have found consistently different effects of these agents on actin conformation in myeloblasts obtained from leukemic patients. The patients tested all had increases in F-actin in response to 12-O-tetradecanoylphorbol-13-acetate (TPA) and most had decreases in F-actin in response to bryostatin. The data suggests that leukemic myeloblasts have a different cytoskeletal response to a tumor promoter and an antineoplastic agent despite their common receptor.
Sham
R L
RL
Department of Medicine and Biophysics, University of Rochester Medical Center, NY 14642.
Packman
C H
CH
Abboud
C N
CN
Lichtman
M A
MA
D000199:Actins / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000970:Antineoplastic Agents; D001853:Bone Marrow / Q000473:pathology; D054713:Bryostatins; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007783:Lactones / Q000494:pharmacology*; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism* / Q000473:pathology; D018942:Macrolides; D008297:Male; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D011487:Protein Conformation / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism / Q000473:pathology
D000199:Actins; D000970:Antineoplastic Agents; D054713:Bryostatins; D007783:Lactones; D018942:Macrolides; C046785:bryostatin 1; D013755:Tetradecanoylphorbol Acetate