title: In vivo study on medullary H(+)-sensitive neurons.
abstract: Using the micro pressure ejection technique, we examined responses of medullary neurons with nonphasic discharges (164 units) to direct application of acidified mock cerebrospinal fluid (CSF, pH 6.85-7.05) in decerebrated spontaneously breathing cats. We found 16 H(+)-sensitive cells; they were excited promptly on application of approximately 500 pl of acidified mock CSF in the vicinity of the neuron under investigation, whereas they were unaffected by microejection of the control mock CSF (pH 7.25-7.60). Of the 16 H(+)-sensitive cells, 10 units were further found to be excited by transcapillary stimulation of the central chemoreceptors by using a method of intravertebral arterial injection of CO2-saturated saline. The discharges increased in a similar time course to that of ventilatory augmentation. Distributions of these 10 specific H(+)-sensitive cells were found in the vicinity of nucleus tractus solitarii as well as deep in the ventrolateral medulla. The present results suggest a possibility that pH-dependent central chemoreceptors, if any, would be located in two distinct medullary regions described in this study.
authors: Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan.
authors: N
authors: N
authors: Kogo
authors: H
authors: H
authors: Arita
mesh_terms: D000818:Animals; D002415:Cats; D002628:Chemoreceptor Cells / Q000502:physiology; D006859:Hydrogen / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D008526:Medulla Oblongata / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D013238:Stereotaxic Techniques
chemical_list: D006859:Hydrogen