Optimizing parameters for correlative immunogold localization by video-enhanced light microscopy, high-voltage transmission electron microscopy, and field emission scanning electron microscopy.
Correlative video-enhanced light microscopy, high-voltage transmission electron microscopy, and low-voltage high resolution scanning electron microscopy were used to examine the binding of colloidal gold-labeled fibrinogen to platelet surfaces. Optimal conditions for the detection of large (18 nm) and small (3 nm) gold particles are described.
Simmons
S R
SR
Department of Veterinary Science, University of Wisconsin, Madison 53706.
Pawley
J B
JB
Albrecht
R M
RM
D001792:Blood Platelets / Q000378:metabolism / Q000648:ultrastructure; D005340:Fibrinogen / Q000378:metabolism; D006046:Gold; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D008854:Microscopy, Electron / Q000379:methods*; D008855:Microscopy, Electron, Scanning
D006046:Gold; D005340:Fibrinogen