Separation and biochemical characterization of blood group N antigen precursor glycoproteins with Thomsen-Friedenreich (T) activity, T-active glycoproteins and N antigen precursor glycoproteins from ascites of primary ovarian cancer patients.
1. Three perchloric acid-soluble fractions from ascites of three primary ovarian cancer patients were subjected to Sephacryl S-300 gel filtration, respectively, and three Fr. 1 which were eluted in the vicinity of void volume as minor fractions, were then separated by a systematic affinity chromatography using Vicia unijuga lectin-Sepharose CL-4B column and Arachis hypogaea lectin-Sepharose CL-4B column into three glycoproteins, blood group N antigen precursor glycoprotein with Thomsen-Friedenreich (T) activity, T-active glycoprotein and N antigen precursor glycoprotein, respectively. 2. These nine glycoproteins separated in yields of 0.1-1.3 mg per 100 ml of ascites, were demonstrated to be mucin-type glycoproteins with Mw of 1,791,000-4,921,000 and contained 33.8-56.1% carbohydrates.
Hayashi
S
S
Department of Biochemistry, Tokyo College of Pharmacy, Japan.
Ohyama
K
K
Yanagi
K
K
Yamakawa
T
T
Watanabe
H
H
Hirakawa
S
S
Ohkuma
S
S
D000596:Amino Acids / Q000032:analysis; D000951:Antigens, Neoplasm / Q000737:chemistry / Q000302:isolation & purification*; D015295:Antigens, Tumor-Associated, Carbohydrate; D001201:Ascites / Q000276:immunology; D002241:Carbohydrates / Q000032:analysis; D004187:Disaccharides / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D005260:Female; D006023:Glycoproteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007519:Isoantigens / Q000737:chemistry / Q000302:isolation & purification; D008951:MNSs Blood-Group System / Q000276:immunology*; D008970:Molecular Weight; D010051:Ovarian Neoplasms / Q000097:blood / Q000276:immunology*; D011498:Protein Precursors / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification
D000596:Amino Acids; D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D002241:Carbohydrates; D004187:Disaccharides; D006023:Glycoproteins; D007519:Isoantigens; D008951:MNSs Blood-Group System; D011498:Protein Precursors; C016467:Thomsen-Friedenreich antigen