Flavonoid modulation of murine neutrophil cytokinesis.
Flavonoids are known to produce a wide range of immunomodulatory effects. In this study, flavone and six hydroxylated analogues were examined for their effect on the FMLP-directed and the random migration of murine peritoneal exudate neutrophils. Flavone significantly (p less than 0.01) inhibits both directed and random migration at an assay concentration of 100 microM. In contrast, fisetin, kaempferol, chrysin, flavonol, morin and quercetin (in decreasing order of activity) significantly (p less than 0.05 to less than 0.01) enhance both directed and random migration at concentrations of 1.0 to 100 microM. Hydrophobicity does not appear to play a key role in the observed compound activity but the number and position of the hydroxyl substitutions might be important. In addition, the [Ca++] modulator chlorpromazine was found to significantly (10 microM; p less than 0.01) inhibit fisetin-enhanced FMLP-directed migration.
Kenny
M T
MT
MerrellDow Research Institute, Indianapolis, Indiana 46268-0460.
Balistreri
F J
FJ
Torney
H L
HL
D000276:Adjuvants, Immunologic; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D002746:Chlorpromazine / Q000494:pharmacology; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D044948:Flavonols; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology / Q000502:physiology; D013329:Structure-Activity Relationship
D000276:Adjuvants, Immunologic; D005419:Flavonoids; D044948:Flavonols; D009240:N-Formylmethionine Leucyl-Phenylalanine; D002118:Calcium; D002746:Chlorpromazine