Expression of cellular-yes protein in mammalian tissues.
Expression of the c-yes proto-oncogene in normal mammalian tissues was studied by immunohistochemistry and by immunoprecipitation assays using affinity purified anti-yes IgG. The antibody was raised against the amino-terminal domain of the human c-yes protein expressed in bacteria as a trpE-yes fusion protein. We detected p62c-yes in a variety of rat and human tissues but its relative level of expression varied significantly among different cell types. Certain epithelial cells, platelets and spermatid acrosomes showed the highest levels of p62c-yes. Intracellular localization of p62c-yes in epithelial cells differed among organs performing different physiological functions. The overall pattern of expression suggests that p62c-yes may be involved in numerous cellular pathways.
Zhao
Y H
YH
Laboratory of Molecular Oncology, Rockefeller University, New York, New York 10021.
Krueger
J G
JG
Sudol
M
M
D000177:Acrosome / Q000378:metabolism; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000941:Antigens / Q000276:immunology; D001792:Blood Platelets / Q000378:metabolism; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004848:Epithelium / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D007163:Immunosorbent Techniques; D008297:Male; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000032:analysis* / Q000235:genetics; D051575:Proto-Oncogene Proteins c-yes; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000276:immunology; D014018:Tissue Distribution; D019061:src-Family Kinases
D000906:Antibodies; D000941:Antigens; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D051575:Proto-Oncogene Proteins c-yes; D019061:src-Family Kinases