Antibody accumulation in small tissue samples: assessment by quantitative autoradiography.
Uptake of radiolabeled 125I monoclonal antibodies in small metastases can only be characterized by autoradiographic techniques. To obtain quantitative data out of autoradiographic images, a transformation of the essentially two-dimensional signal into the Bq per unit volume information is needed. Part of the calibration problem could be solved by using tissue-equivalent standard preparations. However, when aiming at a quantification of radioactivity in small areas (less than or equal to 2 mm diameter), special criteria had to be expanded upon for the reconstruction of the area in the dose matrix and for the correct integration of the radioactivity content.
Schmid
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U
Institute of Radiology and Pathophysiology, German Cancer Research Center, Heidelberg.
Bihl
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H
Matzku
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S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics*; D001345:Autoradiography; D002460:Cell Line; D007457:Iodine Radioisotopes; D008223:Lymphoma / Q000378:metabolism*; D051379:Mice; D008808:Mice, Inbred CBA; D009368:Neoplasm Transplantation
D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes