Shuttling of the autoantigen La between nucleus and cell surface after uv irradiation of human keratinocytes.
During the past years we have established that the nuclear autoantigen La shuttles between the nucleus and the cytoplasm in tumor cells after inhibition of transcription or virus infection. We reinvestigated this shuttling using primary human keratinocytes from both healthy donors and patients with xeroderma pigmentosum. Ultraviolet irradiation resulted in both an inhibition of transcription and a translocation of La protein from the nucleus to the cytoplasm. After a prolonged inhibition of transcription La protein relocated into the nucleus and assembled with nuclear storage regions. The uv-induced shuttling included a translocation to the cell surface, where La protein colocalized with epidermal growth factor receptors.
Bachmann
M
M
Institut für Physiologische Chemie, Universität, Mainz, Federal Republic of Germany.
Chang
S
S
Slor
H
H
Kukulies
J
J
Müller
W E
WE
D001324:Autoantigens / Q000235:genetics / Q000378:metabolism* / Q000493:pharmacokinetics; D001692:Biological Transport / Q000502:physiology / Q000528:radiation effects; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism / Q000528:radiation effects; D002467:Cell Nucleus / Q000276:immunology / Q000378:metabolism / Q000528:radiation effects; D003593:Cytoplasm / Q000276:immunology / Q000378:metabolism / Q000528:radiation effects; D004815:Epidermal Growth Factor / Q000378:metabolism; D066246:ErbB Receptors / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism / Q000528:radiation effects* / Q000648:ultrastructure; D014176:Protein Biosynthesis / Q000528:radiation effects; D012261:Ribonucleoproteins; D014158:Transcription, Genetic / Q000528:radiation effects; D014466:Ultraviolet Rays; D000097585:SS-B Antigen
D001324:Autoantigens; D004815:Epidermal Growth Factor; D066246:ErbB Receptors; D012261:Ribonucleoproteins; D000097585:SS-B Antigen