Detection of the Philadelphia chromosome in interphase nuclei.
Double fluorescence in situ hybridization was used to detect Philadelphia (Ph) chromosomes in interphase nuclei and metaphases of patients with chronic myeloid leukemia. Application of cosmid probes for 3' ABL and 5' BCR sequences gave better results than libraries for chromosomes 9 and 22. The present approach may provide an alternative method for monitoring minimal residual disease in Ph+ CML patients.
Arnoldus
E P
EP
Department of Cytochemistry and Cytometry, Leiden University, The Netherlands.
Wiegant
J
J
Noordermeer
I A
IA
Wessels
J W
JW
Beverstock
G C
GC
Grosveld
G C
GC
van der Ploeg
M
M
Raap
A K
AK
D003360:Cosmids; D006801:Humans; D007150:Immunohistochemistry; D007399:Interphase; D007621:Karyotyping; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D010677:Philadelphia Chromosome