[The experimental study for autoperfused heart-lung preservation--evaluation of pulmonary function after transplantation].
Although various methods for keeping long term good pulmonary function have so far been studied, no reliable method for heart-lung preservation has been established yet. Recently, we aimed at heart-lung preservation using the autoperfusion method and mainly examined pulmonary function after transplantation. After preserving heart-lung organ blocks extracted from mongrel dogs by autoperfusion method, 10 left lungs (preserved for 6.8 +/- 0.8 h) were orthotopically transplanted and combined heart-lung transplantation was performed under extracorporeal circulation using 5 heart-lung grafts (preserved for 7.5 +/- 0.5 h). All of lung-transplanted dogs were alive; preserved lungs showed good gas exchange power from immediately after transplantation. Left lungs on the 3rd day showed normal histological findings and chest X-P also revealed good pneumatization of transplanted lungs. The heart-lung transplantation enabled 4 cases to be free from extracorporeal circulation and arterial blood gas analysis also revealed good results [PO2 396.5 +/- 34.7 mmHg, PCO2 35.5 +/- 6.9 mmHg (FiO2 1.0)]. Lung tissue findings after 6 hours from the above freedom showed slight edema alone. The above results suggested the effectiveness of the autoperfusion method for heart-lung preservation.
Matsuoka
M
M
Department of Thoracic and Cardiovascular Surgery, Mie University School of Medicine, Japan.
Imura
M
M
Higashi
K
K
Kimura
M
M
Okabe
M
M
Morimoto
T
T
Yada
I
I
Namikawa
S
S
Yuasa
H
H
Kusagawa
M
M
D000818:Animals; D004285:Dogs; D006321:Heart; D016041:Heart-Lung Transplantation; D008168:Lung / Q000502:physiology*; D009926:Organ Preservation / Q000379:methods; D010477:Perfusion / Q000379:methods; D011184:Postoperative Period