Determination of clenbuterol in bovine plasma and tissues by gas chromatography-negative-ion chemical ionization mass spectrometry.
A highly sensitive and specific assay was developed for the determination of clenbuterol in bovine plasma and tissues. Clenbuterol and the internal standard [2H9]clenbuterol were measured by gas chromatography-negative-ion chemical ionization mass spectrometry with methane as the reagent gas. Bovine tissues including muscle, liver, heart, kidney, lung, suet, brain, spinal cord and thymus were ground in a buffer of pH 7 and then extracted using ethyl acetate. After two subsequent purification steps, the cleaned-up organic extract was derivatized with pentafluoropropionic anhydride. The mass spectrometer was set to monitor the abundant ions m/z 368 and 377 of the perfluoroacyl derivatives. This assay was performed with 1 ml of plasma or 0.2 g of tissue. The feasibility of this method was demonstrated by the determination of clenbuterol residues as the femtomole level in a variety of tissues.
Girault
J
J
CEMAF S.A., Poitiers, France.
Fourtillan
J B
JB
D000284:Administration, Oral; D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002976:Clenbuterol / Q000008:administration & dosage / Q000032:analysis / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007668:Kidney / Q000737:chemistry*; D008099:Liver / Q000737:chemistry*; D013950:Thymus Gland / Q000737:chemistry*
D002976:Clenbuterol