In vivo elaboration of CSF in acute inflammation: proportionality to the intensity of the inflammatory stimulus and requirement of T lymphocytes.
The colony-stimulating factor(s) (CSF) that stimulates the in vitro growth of bone marrow granulocyte-monocyte progenitors (CFU-GM) increases in the serum of mice challenged by an aseptic abscess induced by copper rod insertion. The effect of the inflammation on the increase of serum CSF is dose-related. The creation of 3 aseptic abscesses indeed results in a higher and longer elevation of serum CSF than 1 abscess. Serum CSF also increases in parallel with the rise in bone marrow CFU-GM; this is consistent with the CSF playing a role in regulation of haematopoiesis in vivo. From previous studies, it appears that T lymphocytes play a central role in the regulation of haematopoiesis. In order to determine the role of T lymphocytes in the inflammation response, cyclosporin A (CyA), an inhibitor of T lymphocyte function, was given in vivo, 2 days before inflammation induction. CyA abrogates the increase in both serum CSF and CFU-GM. Furthermore, a lower increase in serum CSF was observed in copper-implanted nude mice. These results suggest that the CSF production induced by inflammation requires the functional integrity of T lymphocytes.
Hamood
M
M
Experimental Haematology Laboratories, Free University of Brussels, Belgium.
Chatelain
C
C
Fondu
P
P
Symann
M
M
D000038:Abscess / Q000097:blood / Q000473:pathology; D000818:Animals; D001853:Bone Marrow / Q000473:pathology; D003115:Colony-Stimulating Factors / Q000097:blood*; D003300:Copper; D003524:Cyclosporins / Q000494:pharmacology; D006098:Granulocytes / Q000473:pathology; D006412:Hematopoietic Stem Cells / Q000473:pathology; D007249:Inflammation / Q000097:blood* / Q000209:etiology / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D009000:Monocytes / Q000473:pathology; D013601:T-Lymphocytes / Q000502:physiology*
D003115:Colony-Stimulating Factors; D003524:Cyclosporins; D003300:Copper