[Use of polymerase chain reaction (PCR) to detect monoclonality of B-lymphoproliferative disorders at DNA level].
A technique has been developed using PCR to detect monoclonality of B-lymphoproliferative disorders. DNA was extracted from the tissue, blood and paraffin embedded sections by biochemical means or boiling. Forty cases of B-non Hodgkin's lymphoma (NHL), 15 cases of T-NHL, 8 cases of chronic lymphocytic leukemia, 17 cases of reactive lymphadenopathy and 12 cases of various non-lymphatic tumor were examined. Monoclonality of B-lymphocytes was detected in 86-92% of cases with B-lymphoproliferative diseases, but none in T-NHL, reactive disorders and non-lymphatic tumors. This technique provides a new molecular biologic method to diagnose malignant B-lymphoproliferative disorders. It may be useful in Ig gene rearrangement study, differential diagnosis and retrospective investigation of lymphoproliferative disorders.
Wan
J H
JH
Institute of Hematology, Chinese Academy of Medical Sciences, Tianjin.
D004273:DNA, Neoplasm / Q000032:analysis; D003937:Diagnosis, Differential; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000175:diagnosis*; D016393:Lymphoma, B-Cell / Q000175:diagnosis*; D016399:Lymphoma, T-Cell / Q000175:diagnosis; D008232:Lymphoproliferative Disorders / Q000175:diagnosis*; D016133:Polymerase Chain Reaction
D004273:DNA, Neoplasm