title: Morphology of seminal and swim-up spermatozoa and the outcome of in vitro fertilization and embryo transfer.
abstract: Tubal infertility was treated by in vitro fertilization-embryo transfer (IVF-ET) in 112 couples. Twenty-eight pregnancies were obtained in 140 treatment cycles. Couples are accepted for treatment in our IVF-ET programme if previous semen samples fulfil the inclusion criteria: ejaculate volume greater than 1.5 ml, concentration of spermatozoa greater than 15 x 10(6) ml-1, greater than 40% motile spermatozoa, and greater than 25% spermatozoa with normal morphology. In order to determine to which extent IVF-ET treatment results are influenced by sperm morphology, within this selected group of patients, we have retrospectively analysed the data from both original semen samples and swim-up preparations. The sperm morphology was not related to the outcome of treatment in terms of fertilization (ovum cleavage rate), early embryo development, or pregnancy. Nor was any relationship detected between early embryo development or pregnancy and the degree of improvement in morphology resulting from the swim-up procedure. However, if improvement in morphology by swim-up was high, ovum cleavage rate was low. Sperm morphology within the limits set by our inclusion criteria could not predict the outcome of IVF-ET treatment. It is further concluded that the presence of abnormal spermatozoa at the site of fertilization may be without harm if only the number of normal sperms is high enough.
authors: Department of Obstetrics and Gynaecology, Karolinska Institutet, Huddinge University Hospital, Sweden.
authors: L
authors: L
authors: Rosenborg
authors: O
authors: O
authors: Gustafson
authors: N O
authors: NO
authors: Lunell
authors: L
authors: L
authors: Nylund
authors: A
authors: A
authors: Pousette
authors: H
authors: H
authors: Slotte
authors: E
authors: E
authors: Akerlöf
authors: B
authors: B
authors: Fredricsson
mesh_terms: D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D009865:Oocytes / Q000502:physiology; D011247:Pregnancy; D012661:Semen / Q000502:physiology; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*