title: Combined GTG-banding and nonradioactive in situ hybridization improves characterization of complex karyotypes.
abstract: Nonradioactive in situ hybridization (ISH) using biotinylated centromere probes for chromosomes 1, 6, 7, 10, 16, 17, 18, and the X, respectively, was combined with GTG-banding to study cytogenetic changes in two different ovarian cancer cell lines. ISH was performed after GTG-banding on the same metaphase. The use of a low trypsin concentration (0.01%) in the banding procedure was essential for subsequent ISH. This combined approach allows the detection of subtle chromosomal rearrangements and appears to aid the identification of marker chromosomes.
authors: Department of Pathology, Leiden University The Netherlands.
authors: V T
authors: VT
authors: Smit
authors: J W
authors: JW
authors: Wessels
authors: P
authors: P
authors: Mollevanger
authors: P I
authors: PI
authors: Schrier
authors: A K
authors: AK
authors: Raap
authors: G C
authors: GC
authors: Beverstock
authors: C J
authors: CJ
authors: Cornelisse
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D002503:Centromere; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D015342:DNA Probes; D005260:Female; D006801:Humans; D007621:Karyotyping / Q000379:methods*; D008297:Male; D009693:Nucleic Acid Hybridization; D010051:Ovarian Neoplasms / Q000235:genetics*; D014357:Trypsin / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D015342:DNA Probes; D014357:Trypsin