Iron-regulated outer membrane protein of Bacteroides fragilis involved in heme uptake.
Under iron starvation, Bacteroides fragilis expresses various iron-regulated outer membrane proteins. In this study, a deferrated minimal medium was used in growth experiments, and the role of one of these iron-regulated outer membrane proteins (a 44-kDa protein) in an iron uptake mechanism which acquires iron from heme compounds was elucidated. When a specific 44-kDa protein antiserum was used in a medium with heme as the only iron source, growth inhibition was observed. These results demonstrate that the 44-kDa outer membrane protein plays an important role in the uptake of heme in B. fragilis.
Otto
B R
BR
Department of Medical Microbiology, Medical School Vrije Universiteit, Amsterdam, The Netherlands.
Sparrius
M
M
Verweij-van Vught
A M
AM
MacLaren
D M
DM
D000818:Animals; D001425:Bacterial Outer Membrane Proteins; D001439:Bacteroides / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media; D006418:Heme / Q000378:metabolism*; D006427:Hemin / Q000494:pharmacology; D006454:Hemoglobins / Q000494:pharmacology; D007501:Iron / Q000502:physiology*; D011817:Rabbits
D001425:Bacterial Outer Membrane Proteins; D003470:Culture Media; D006454:Hemoglobins; D006418:Heme; D006427:Hemin; D007501:Iron