Adherent synovial cells from nonrheumatoid arthritis do not release interleukin 1 beta and prostaglandin E2 spontaneously in longterm culture.
Adherent synovial cells from both 13 patients without rheumatoid arthritis (RA) (gout, osteoarthritis and meniscal lesion) and 8 patients with RA consisted of dendritic cells, macrophage-like cells and fibroblast-like cells after cloning in a similar fashion as reported in our previous paper. All the adherent synovial cells from patients without RA did not release interleukin 1 (IL-1) beta and prostaglandin E2 (PGE2) spontaneously, while those cells released comparable amounts of IL-1 beta, but not PGE2 to RA cells after type II collagen stimulation. Only the synovial cells from RA, irrespective of morphology and cloning, released IL-1 beta and PGE2 without stimulation. Nonrheumatoid synovial cells may differ functionally from RA cells.
Goto
M
M
Institute of Rheumatology, Tokyo Women's Medical College, Japan.
Okamoto
M
M
Sasano
M
M
Yanagisawa
S
S
Miyamoto
T
T
Nishioka
K
K
Nakamura
K
K
Aotuka
S
S
Kawakami
N
N
Yokohari
R
R
D001172:Arthritis, Rheumatoid / Q000378:metabolism / Q000473:pathology; D002448:Cell Adhesion; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology; D015232:Dinoprostone / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D006073:Gout / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D010003:Osteoarthritis / Q000378:metabolism* / Q000473:pathology; D012016:Reference Values; D012867:Skin / Q000166:cytology / Q000378:metabolism; D013583:Synovial Membrane / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors
D007375:Interleukin-1; D003094:Collagen; D015232:Dinoprostone