gCap39, a calcium ion- and polyphosphoinositide-regulated actin capping protein.
The polymerization of actin filaments is involved in growth, movement, and cell division. It has been shown that actin polymerization is controlled by gelsolin, whose interactions with actin are activated by calcium ion (Ca2+) and inhibited by membrane polyphosphoinositides (PPI). A smaller Ca2(+)- and PPI-regulated protein, gCap39, which has 49% sequence identity with gelsolin, has been identified by cDNA cloning and protein purification. Like gelsolin, gCap39 binds to the fast-growing (+) end of actin filaments. However, gCap39 does not sever actin filaments and can respond to Ca2+ and PPI transients independently, under conditions in which gelsolin is ineffective. The coexistence of gCap39 with gelsolin should allow precise regulation of actin assembly at the leading edge of the cell.
Yu
F X
FX
Department of Physiology, University of Texas Southwestern Medical Center, Dallas 75235-9040.
Johnston
P A
PA
Südhof
T C
TC
Yin
H L
HL
D000199:Actins / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015723:Gene Library; D006801:Humans; D007668:Kidney / Q000378:metabolism; D007700:Kinetics; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008840:Microfilament Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D012689:Sequence Homology, Nucleic Acid
D000199:Actins; D008840:Microfilament Proteins; D009687:Nuclear Proteins; C483826:Capg protein, mouse; C066575:CAPG protein, human; D004247:DNA