Development of an artificial extracellular matrix. Solution castable polymers with cell recognizable peptidyl side chain.
This article reports a novel cell-adhesion-promoting polymeric system as an adhesive-protein model. The characteristic features of the system are that polymers have recognition sites and are solution-castable on material surfaces. These co-polymers were prepared by radical co-polymerization of styrene with peptidyl macromer, which include an Arg-Gly-Asp (RGD) sequence, the minimal core peptide recognizable by cell adhesion receptors. Upon casting and subsequent immersion into water, RGD-peptide chains were enriched at the interface with water. Bovine endothelial cells (ECs) adhered to the cast surface, and adhesion was partly directed by RGD-recognition mechanisms. These co-polymers, incorporated with artificial bioactive ligands, may provide an artificial extracellular matrix (ECM) for implantable devices and hybrid organs using a conventional solution-casting technique.
Ozeki
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Department of Bioengineering, National Cardiovascular Center, Osaka, Japan.
Matsuda
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T
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000502:physiology; D004730:Endothelium, Vascular / Q000166:cytology*; D005109:Extracellular Matrix / Q000502:physiology*; D010455:Peptides / Q000502:physiology*; D011108:Polymers; D013329:Structure-Activity Relationship
D015815:Cell Adhesion Molecules; D010455:Peptides; D011108:Polymers