Nucleotide sequence of the F plasmid gene, traC, and identification of its product.
The traC gene of the F plasmid tra operon is required for the assembly of mature F-pilin subunits into extended F pili. The nucleotide sequence of traC was determined with a determined with a deduced coding region of 875 amino acids (aa) and 99066 Da. The traC1044 mutant allele, which allows filamentous phage infection in the absence of piliation, contains a C-to-T transition leading to an Arg----Cys substitution. Confirmation of the translational start came from the direct N-terminal aa sequencing of a TraC-alkaline phosphatase fusion protein.
Schandel
K A
KA
Department of Biochemistry, Duke University Medical Center, Durham, NC 22710.
Maneewannakul
S
S
Vonder Haar
R A
RA
Ippen-Ihler
K
K
Webster
R E
RE
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D005144:F Factor / Q000235:genetics*; D036781:Fimbriae Proteins; D008969:Molecular Sequence Data; D010957:Plasmids; D014176:Protein Biosynthesis
D001426:Bacterial Proteins; C074790:traC protein, Plasmid F; D036781:Fimbriae Proteins