[A trial of a cultured inactivated vaccine against hepatitis A on Saguinus mystax tamarins].
An experimental batch of inactivated hepatitis A vaccine was prepared using hepatitis A virus (HAV), HAS-15 strain, adapted to cell culture and purified by ultracentrifugation. The vaccine was tested in tamarins immunized intramuscularly three times one month apart. Three tamarins received a vaccine preparation containing 10 ng of immunogen each, three--100 ng each, and three animals were used as controls. The efficacy was judged by the anti-HAV antibody response in the vaccinated animals and development of immunity to subsequent virus challenge two months after the last immunization. The criteria of infection were: elevation of serum alanine aminotransferase, fecal excretion of HAV and production of specific antibody of IgM class. The immune response to 10 ng of the immunogen was lower than to 100 ng, however, both doses produced complete resistance to infection. The booster effect was observed in animals receiving 10 ng of the immunogen. The vaccine batch under study in the indicated doses was shown to have a good immunogenic potency and protective activity for tamarins.
Poleshchuk
V F
VF
Balaian
M S
MS
Sobol'
A V
AV
Titova
I P
IP
Guliaeva
T V
TV
Dokin
V P
VP
Kusov
Iu Iu
IuIu
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D004306:Dose-Response Relationship, Immunologic; D004353:Drug Evaluation, Preclinical; D005243:Feces / Q000737:chemistry; D005260:Female; D006506:Hepatitis A / Q000276:immunology / Q000517:prevention & control*; D006508:Hepatitis Antibodies / Q000097:blood; D007114:Immunization; D008297:Male; D012452:Saguinus / Q000276:immunology*; D013997:Time Factors; D015164:Vaccines, Inactivated / Q000276:immunology; D014761:Viral Hepatitis Vaccines / Q000276:immunology*
D000956:Antigens, Viral; D006508:Hepatitis Antibodies; D015164:Vaccines, Inactivated; D014761:Viral Hepatitis Vaccines; D000410:Alanine Transaminase
Ispytanie na tamarinakh Saguinus mystax kul'tural'noĭ inaktivirovannoĭ vaktsiny protiv gepatita A.