[The nature of N-terminal signal sequence determines the type of intracellular distribution and effectiveness of export of human growth hormone in Saccharomyces cerevisiae].
Various N-terminal signal peptides (SP) were tested to investigate a human growth hormone (hGH) synthesis, processing and intracellular sorting in yeast. Maximal level of hGH was observed in the case when the mature hGH gene was placed under the control of PHO5 promoter. In this case about 90% of hGH was localized in the cytosol, but some portion was trustworthly detected in microsomes and periplasma in spite of the absence of SP. Addition of own or PHO5 SP resulted in lowering of the synthesis and a difficulty in the prehGH processing. In this case the immunoreactive products were localized mainly in periplasma and vacuoles and to a lesser degree in the cytosol. When hGH gene was placed under the control of the yeast MF alpha 1 promoter and alpha-factor preprosegment was used as SP more then a half (67%) of hGH processed correctly was exported in a medium, the rest was detected in vacuole (17%) and periplasma (8%).
Tsiomenko
A B
AB
Lupashin
V V
VV
Morzunov
S A
SA
Karpychev
I V
IV
El'darov
M A
MA
Bezhelarskaia
S N
SN
Rubtsov
P M
PM
Skriabin
K G
KG
Kulaev
I S
IS
D000595:Amino Acid Sequence; D001483:Base Sequence; D005818:Genetic Engineering; D005822:Genetic Vectors; D013006:Growth Hormone / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D010957:Plasmids; D021382:Protein Sorting Signals / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D013006:Growth Hormone
Priroda N-kontsevoĭ signal'noĭ posledovatel'nosti opredeliaet kharakter vnutrikletochnogo raspredeleniia i éffektivnost' éksporta gormona rosta cheloveka u drozhzheĭ Saccharomyces cerevisiae.