Sodium butyrate inhibits c-myc and stimulates c-fos expression in all the steps of the cell-cycle in hepatoma tissue cultured cells.
Sodium butyrate decreases the c-myc mRNA and increases the c-fos transcript level in HTC cells. This effect is independent of the cell-cycle phase. Actinomycin D suppresses the effect on c-fos. Cycloheximide increases both mRNA levels. Sodium butyrate suppresses the effect on c-myc and potentializes the effect on c-fos mRNAs. This suggests that sodium butyrate acts at the transcriptional level and that its effect does not result from the arrest of the cells at the G1 phase.
Tichonicky
L
L
Institut Cochin de Génétique Moléculaire, URA 1147 CNRS, Paris.
Kruh
J
J
Defer
N
N
D000818:Animals; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002453:Cell Cycle; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D016259:Genes, myc / Q000187:drug effects*; D008114:Liver Neoplasms, Experimental; D009857:Oncogenes / Q000187:drug effects*; D012333:RNA, Messenger / Q000187:drug effects; D012334:RNA, Neoplasm / Q000187:drug effects; D014158:Transcription, Genetic / Q000187:drug effects; D014407:Tumor Cells, Cultured
D002087:Butyrates; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D020148:Butyric Acid; D003609:Dactinomycin; D003513:Cycloheximide