Purification and immunochemical characterization of a rat liver sulphotransferase conjugating paracetamol.
Paracetamol sulphotransferase (ST) was purified 250-fold from male rat liver, and the pure enzyme used to elicit antibodies in rabbit. The enzyme was active towards paracetamol at pH 9.0, as well as towards several commonly used drugs, and formed sulphates at both O- and N-atoms. Comparison of the substrate specificity of paracetamol ST with that of aryl sulphotransferases isolated by other workers suggested that we have purified a previously unknown isoenzyme of rat liver ST, although the difficulties of characterization of STs based on their substrate specificities is noted. The antibody preparation recognized only one polypeptide (Mr = 35,000) on immunoblot analysis of rabbit liver cytosol, corresponding to purified paracetamol ST. Analysis of the tissue distribution of this protein demonstrated that its expression was restricted to the liver, as was the enzyme activity. The observed sex difference in paracetamol ST (males greater than females) was determined by immunoblot analysis to be the result of reduced enzyme protein levels in females. In human liver cytosol, the antibody recognized two polypeptides, probably corresponding to M- and P-phenol STs, suggesting significant sequence similarity between rat and human phenol sulphotransferases.
Coughtrie
M W
MW
Department of Biochemical Medicine, University of Dundee, Ninewells Hospital and Medical School, U.K.
Sharp
S
S
D000082:Acetaminophen / Q000378:metabolism* / Q000493:pharmacokinetics; D000818:Animals; D000906:Antibodies / Q000276:immunology; D015239:Arylsulfotransferase / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D003429:Cross Reactions; D005260:Female; D006801:Humans; D008099:Liver / Q000201:enzymology*; D008297:Male; D051381:Rats; D013379:Substrate Specificity; D014018:Tissue Distribution
D000906:Antibodies; D000082:Acetaminophen; D015239:Arylsulfotransferase