title: NMR and computational studies of interactions between remote residues in gangliosides.
abstract: Conformational preferences of the gangliosides GM1, GM1b, and GD1a have been investigated by using a systematic combination of NMR distance constraints and molecular mechanics calculations. These gangliosides share a common four-sugar core but differ in the number or placement of sialic acid residues attached to the core. Placement of the sialic acid residues is shown to influence the preferred core conformation. The origin of these effects is postulated to be intramolecular interactions of the sialic acid residues with other remote residues. In the case of GM1, hydrogen bonds between the internal sialic acid and an N-acetyl group on GalNAc are suggested. In the case of GD1a, a hydrogen-bonding network between the terminal and internal sialic acids is suggested to play a role.
authors: Department of Biochemistry and Molecular Biophysics, Virginia Commonwealth University, Richmond 23298-0614.
authors: J N
authors: JN
authors: Scarsdale
authors: J H
authors: JH
authors: Prestegard
authors: R K
authors: RK
authors: Yu
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics; D000445:Aldehyde Oxidoreductases / Q000235:genetics; D050727:Benzaldehyde Dehydrogenase (NADP+); D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005677:G(M1) Ganglioside / Q000031:analogs & derivatives* / Q000737:chemistry; D005732:Gangliosides / Q000737:chemistry*; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid; D012794:Sialic Acids / Q000737:chemistry
chemical_list: D005732:Gangliosides; D012794:Sialic Acids; C021298:ganglioside, GD1a; D005677:G(M1) Ganglioside; C029624:ganglioside M1b; D000429:Alcohol Oxidoreductases; C021538:L-mandelate dehydrogenase; D000445:Aldehyde Oxidoreductases; D050727:Benzaldehyde Dehydrogenase (NADP+); D019158:N-Acetylneuraminic Acid