Lack of detectable metabolism for solubilized 2,3,4-trimethylpentane by rat kidney proximal tubules.
Primary proximal tubule suspension cultures exposed to solubilized 2,3,4-trimethylpentane (2,3,4-TMP) resulted in a linear dose response, as determined by cellular lactate dehydrogenase leakage. The EC50 for 2,3,4-TMP was 16.3 mM. Metabolite analysis by gas chromatography/mass spectrometry of supernate and cell extracts from cultures exposed to 2,3,4-TMP (12.0 mM) failed to detect the presence of metabolites. Electron-microscopic examination of proximal tubules exposed to 2,3,4-TMP indicated ultrastructural changes that included increased mitochondrial swelling, increased vesiculation, decreased microvilli and pyknotic nuclei. This study indicates that kidney proximal tubules do not appear to metabolize 2,3,4-TMP.
DelRaso
N J
NJ
NSI Technology Services Corporation-Environmental Sciences, Dayton, OH 45431.
Mattie
D R
DR
Godin
C S
CS
D000818:Animals; D002478:Cells, Cultured; D008401:Gas Chromatography-Mass Spectrometry; D007687:Kidney Tubules, Proximal / Q000201:enzymology / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008928:Mitochondria / Q000473:pathology; D010420:Pentanes / Q000378:metabolism* / Q000633:toxicity; D051381:Rats; D011916:Rats, Inbred F344
D010420:Pentanes; D007770:L-Lactate Dehydrogenase; C053045:2,3,4-trimethylpentane