[Prevention of alcohol-induced gastric mucosal injury by prostaglandin E-1].
Effects of ethanol and prostaglandin on the glycoprotein synthesis in the gastric mucus cells have been evaluated. The rat gastric mucosal cell were subjected to a short-term tissue culture in the presence of ethanol and prostaglandin E2 (Ornoprostil), using [3H]-proline, [3H]-palmitic acid, [3H]-galactosamine. Low concentration of ethanol (0.05-0.01 M) stimulated the glycoprotein synthesis, but higher concentration of ethanol (0.5-1.5 M) caused a marked reduction in the glycoprotein synthesis. Addition of prostaglandin E2, especially addition of prostaglandin prior to ethanol treatment, has improved the glycoprotein synthesis and secretion in the presence of ethanol. It was suggested that addition of prostaglandin resulted in the stabilization of the synthesis and secretion of the mucus glycoprotein in the presence of ethanol.
Tsukada
H
H
Division of Metabolism and Clinical Nutrition, Faculty of Medicine, Kyoto University.
Seino
Y
Y
Jo
I
I
Tatsuta
H
H
Ito
K
K
Hashimoto
A
A
Kotera
T
T
Matsubayashi
Y
Y
Mori
Y
Y
Ito
A
A
D000527:Alprostadil / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D000431:Ethanol / Q000009:adverse effects*; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D006023:Glycoproteins; D000431:Ethanol; D000527:Alprostadil