A high molecular weight cytotoxic lymphokine in supernatants of lymphokine-activated killer cells cross-reacts with transforming growth factor beta.
Supernatants of lymphokine-activated killer (LAK) cells were highly cytotoxic for melanoma A375 cells. A high-molecular-weight fraction was isolated from such supernatants by gel filtration on an S-300 Sephacryl column (Fraction 1; Fr1). The cytotoxic activity in Fr1 was heat- and acid-resistant and was completely abolished by a rabbit antibody against TGF-beta. We conclude that Fr1 contains TGF-beta or a cross-reactive molecule, associated with a high-molecular-weight carrier.
Larisch-Bloch
S
S
Lautenberg Center for General and Tumor Immunology, Hebrew University, Hadassah Medical School, Jerusalem, Israel.
Sterman
B
B
Sulitzeanu
D
D
D000818:Animals; D002850:Chromatography, Gel; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic; D006358:Hot Temperature; D006801:Humans; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology* / Q000378:metabolism; D008222:Lymphokines / Q000276:immunology* / Q000302:isolation & purification; D046911:Macromolecular Substances; D008970:Molecular Weight; D016212:Transforming Growth Factor beta / Q000276:immunology*; D014407:Tumor Cells, Cultured
D008222:Lymphokines; D046911:Macromolecular Substances; D016212:Transforming Growth Factor beta