title: Stoichiometry of elongation factor G function in translation.
abstract: A steady-state translation system has been used in vitro to measure the stoichiometry with which elongation factor G-GTP complexes are dissipated during polypeptide elongation. It has been possible to separate this dissipation from that associated with elongation factor Tu function. Our measurements for the wild-type as well as for two mutant variants indicate that there is one elongation factor G-GTP complex dissipated per peptide bond in the steady-state.
authors: Department of Molecular Biology, Uppsala University, Sweden.
authors: A A
authors: AA
authors: Dahlfors
authors: C G
authors: CG
authors: Kurland
mesh_terms: D002250:Carbon Radioisotopes; D006160:Guanosine Triphosphate / Q000378:metabolism; D007700:Kinetics; D020653:Peptide Elongation Factor G; D010445:Peptide Elongation Factors / Q000378:metabolism*; D010649:Phenylalanine / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D014176:Protein Biosynthesis; D012360:RNA, Transfer, Phe / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*
chemical_list: D002250:Carbon Radioisotopes; D020653:Peptide Elongation Factor G; D010445:Peptide Elongation Factors; D010710:Phosphates; D010761:Phosphorus Radioisotopes; D012360:RNA, Transfer, Phe; D010649:Phenylalanine; D006160:Guanosine Triphosphate