Direct cloning of the human genomic apolipoprotein E gene using magnetic separation of single-stranded DNA.
Solid-phase methods can be used for direct cloning of in vitro-amplified genomic DNA with high efficiency, without the need for restriction enzymes or ligase. Single-stranded DNA fragments are generated by magnetic separation and are simply mixed with single-stranded vector to form gap-duplex molecules that can be readily transformed. This strategy, in combination with direct solid-phase DNA sequencing, was used to analyze individual alleles in the human apolipoprotein E locus.
Hornes
E
E
Dynal A.S., Oslo.
Hultman
T
T
Moks
T
T
Uhlén
M
M
D000595:Amino Acid Sequence; D001057:Apolipoproteins E / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D004277:DNA, Single-Stranded / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D008280:Magnetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D001057:Apolipoproteins E; D004277:DNA, Single-Stranded