Rapid isolation of substrate-quality plasmid DNA without CsCl-dye density gradients.
The isolation of plasmid DNA produced in transformed bacterial cells is essential for many molecular biology techniques. Two drawbacks to the widely used CsCl-ethidium bromide method of preparation are the need for ultracentrifuge time and the generation of ethidium bromide waste. In this article we describe a method for the quick isolation of plasmid DNA without the use of an ultracentrifuge or ethidium bromide.
Aguilar-Cordova
E
E
Department of Pathology, School of Medicine, University of California, Davis 95616.
Fontes
J
J
D002499:Centrifugation, Density Gradient; D002586:Cesium; D002712:Chlorides; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004926:Escherichia coli / Q000235:genetics; D005821:Genetic Techniques; D010957:Plasmids; D014169:Transformation, Bacterial
D002712:Chlorides; D004269:DNA, Bacterial; D002586:Cesium; C028019:cesium chloride