Use of microwave fixation in the preparation of cell cultures for observation with the scanning electron microscope.
This paper describes a method for primary fixation of cultured cells for scanning (SEM) and transmission (TEM) electron microscopy using microwaves alone. This method of fixation takes 8 seconds and is therefore quicker and less expensive than conventional fixation techniques. The preservation of cell morphology is excellent and cultures of mammalian immune system cells and peripheral nervous tissue have been examined using this fixation.
Argall
K
K
School of Biological Sciences, University of Sydney, New South Wales, Australia.
Armati
P
P
D000818:Animals; D000831:Animals, Newborn; D002450:Cell Communication; D002478:Cells, Cultured; D005347:Fibroblasts / Q000648:ultrastructure*; D005727:Ganglia, Spinal / Q000166:cytology; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D008872:Microwaves; D011309:Preservation, Biological; D051381:Rats; D011917:Rats, Inbred Lew; D012583:Schwann Cells / Q000648:ultrastructure*; D013601:T-Lymphocytes / Q000648:ultrastructure*