High-performance liquid chromatography for the separation of angiotensin and its metabolites in human plasma and sweat.
A reversed-phase high-performance liquid chromatography (HPLC) method with gradient elution for the separation of angiotensin peptides is described. The highly reproducible method allows the base-line separation of angiotensin peptides with UV detection at 225 nm. This chromatographic methodology in combination with radioimmunoassay (RIA) is used for the characterization of angiotensin peptides in human plasma and sweat.
Hermann
K
K
Department of Dermatology, Ludwig Maximilians University Münich, West Germany.
Ring
J
J
Phillips
M I
MI
D000097:Acetonitriles; D000804:Angiotensin II / Q000032:analysis / Q000097:blood* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D000432:Methanol; D011863:Radioimmunoassay; D013542:Sweat / Q000737:chemistry*
D000097:Acetonitriles; D000804:Angiotensin II; D000432:Methanol; C032159:acetonitrile