title: [Fluid shear stress effects on intracellular calcium concentrations in cultured vascular endothelial cells].
abstract: Vascular endothelial cells are known to modulate their functions in response not only to humoral stimuli but also to such physical stimuli as fluid shear stress generated by blood flow. However, the mechanisms by which the hemodynamic force acts on endothelial cells are not yet well understood. We have studied how endothelial cells recognize the shear stress and mediate it to intracellular organelles. Cultured monolayers of bovine aortic endothelial cells loaded with the highly fluorescent Ca+(+)-sensitive dye Fura 2 were exposed to different levels of fluid shear stress in a specially designed flow chamber and simultaneous changes in intracellular-free Ca+(+) concentration were measured using photometric fluorescence microscopy. Application of shear stress to cells by fluid perfusion led to an immediate several-fold increase in Ca+(+) concentration within 1 min, followed by a rapid decline, and finally a plateau somewhat higher than control levels during the entire period of the stress application. The early part of the response, but no plateau, was observed even in Ca+(+)-free medium added with 2 mM EDTA, and in the presence of calcium antagonists (e.g. 2 x 10(-5) M nicardipine). Thus, endothelial cells may have a flow-sensing property which recognize the shear stress on the membrane as a stimulus and mediates the signal to increase intracellular free Ca(+)+ which is a major component of the internal signalling system of the cell.
authors: Research Institute of Applied Electricity, Hokkaido University.
authors: J
authors: J
authors: Ando
authors: A
authors: A
authors: Ohtsuka
authors: Y
authors: Y
authors: Katayama
authors: S
authors: S
authors: Araya
authors: A
authors: A
authors: Kamiya
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D001775:Blood Circulation; D002118:Calcium / Q000378:metabolism* / Q000502:physiology; D002417:Cattle; D002450:Cell Communication; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D006439:Hemodynamics; D015290:Second Messenger Systems; D013312:Stress, Physiological
chemical_list: D002118:Calcium