Aldehyde dehydrogenase activity in xenografted human brain tumor in nude mice. Preliminary results in human glioma biopsies.
ALDH activity measured fluorimetrically using a high concentration of aliphatic aldehyde as substrate was studied in human glioblastomas grafted in nude mice. Compared with normal brain, ALDH activity is significantly increased in malignant glioma tissue, especially in the cytosolic subcellular fraction. Correlatively, in comparison with normal brain tissue, MDA levels were significantly reduced in whole homogenates and in cytosolic fractions of xenografted glioblastoma tissue. Preliminary results concerning human malignant glioma biopsies are in good agreement with our experimental data. In view of previous works, these results suggest a relationship between alterations in ALDH iso-enzymes activities and cytosolic aldehyde concentrations with respect to normal or tumoral cell growth.
Quemener
V
V
Department of Cell Biology, Centre Hospitalier Universitaire de Rennes, France.
Moulinoux
J P
JP
Martin
C
C
Darcel
F
F
Guegan
Y
Y
Faivre
J
J
Quash
G A
GA
D000444:Aldehyde Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000447:Aldehydes / Q000627:therapeutic use; D000818:Animals; D001706:Biopsy; D001932:Brain Neoplasms / Q000188:drug therapy / Q000201:enzymology* / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D005910:Glioma / Q000188:drug therapy / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D008315:Malondialdehyde / Q000032:analysis; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D013347:Subcellular Fractions / Q000201:enzymology; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
D000447:Aldehydes; C059170:4-amino-4-methyl-2-pentyne-1-al; D008315:Malondialdehyde; D000444:Aldehyde Dehydrogenase