[Adaptation of permanent bovine kidney cells to serum free hormone supplemented cell culture media].
The examinations show, that it is possible to adapt MDBK-cells (ATCC CCL 22) to serum-poor (0.1% fetal calf serum) and to serum-free, adequate supplemented media. Morphology of the cells remained unchanged. The components of the serum-free medium are a 50:50 mixture of Dulbecco's modified Eagle's medium and HAM's F12 medium, supplemented with insulin (5 micrograms ml-1), transferrin (10 micrograms ml-1), thyroglobulin (10 micrograms ml-1), prostaglandin E1 (50-100 ng ml-1). Seeding cells at a density of 26.10(3) cm-2 at day 0 resulted in better multiplication than a higher seeding density.
Binder
S
S
Lehrstuhl für Tierhygiene und Nutztierkunde, Technischen Universität München, Freising-Weihenstephan.
Beutel
M
M
Lindl
T
T
D000818:Animals; D002417:Cattle; D002455:Cell Division; D002460:Cell Line; D003470:Culture Media; D006133:Growth Substances / Q000378:metabolism*; D006728:Hormones / Q000378:metabolism*; D007668:Kidney / Q000166:cytology*
D003470:Culture Media; D006133:Growth Substances; D006728:Hormones
Adaption permanenter Rindernierenzellen an serumfreies hormonsupplementiertes Zellkulturmedium.