title: A protein marker of hamster oocyte fertilization.
abstract: Using a polyclonal rabbit antiserum directed against guinea-pig heparin-binding placental protein (HBPP), we have demonstrated by indirect immunofluorescence the expression of this antigen by hamster preimplantation embryos. HBPP was localized to blastomeres, but not to the zona pellucida, of hamster preimplantation embryos conceived in vivo. Hamster embryos, from the pronuclear to hatched blastocyst stages, were HBPP-positive with greatest immunofluorescence detected at the pronuclear to 2-cell stages. Thereafter, HBPP-dependent fluorescence diminished. By contrast, mature spermatozoa and unfertilized metaphase II oocytes were HBPP negative. Direct microinjection of spermatozoa or diluent through the zona pellucida and into the ooplasm activated the oocytes to express HBPP. These findings demonstrate that expression of HBPP is a post-conceptional event limited to fertilized (or activated) oocytes and that transcription of paternal genomes was not required for HBPP expression. The distribution and temporal kinetics suggest that HBPP may be secreted or released by activated cortical granules into the perivitelline space and concentrated at the oolemma. Whereas hamster embryos conceived in vivo consistently demonstrated a uniformly distributed immunofluorescence, non-viable and degenerate embryos showed weak and patchy reactions. Thus, we have identified a heparin-interacting protein which may serve as a marker of in-vivo and in-vitro oocyte activation and embryo quality.
authors: Department of Obstetrics and Gynaecology, Royal North Shore Hospital, St Leonards, N.S.W., Australia.
authors: M J
authors: MJ
authors: Sinosich
authors: S E
authors: SE
authors: Lanzendorf
authors: D M
authors: DM
authors: Saunders
authors: G D
authors: GD
authors: Hodgen
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000737:chemistry; D006224:Cricetinae; D005260:Female; D005306:Fertilization / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D008647:Mesocricetus; D009865:Oocytes / Q000737:chemistry*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000032:analysis*
chemical_list: D015415:Biomarkers; D011257:Pregnancy Proteins