Molecular cloning and nucleotide sequence analysis of cholera toxin genes of the CtxA- Vibrio cholerae strain Texas Star-SR.
The ctx operons from the Vibrio cholerae El Tor strain 3083 and its CtxA- derivative Texas Star-SR were cloned, and their nucleotide sequences were compared. A single missense mutation in the Texas Star-SR ctxA cistron which results in the substitution of threonine for alanine at position 191 of the 258-amino-acid CtxA precursor was identified. Immunoblot analysis detected the mutant CtxA intracellularly early in the culture cycle but not extracellularly at any growth stage.
Brickman
T J
TJ
Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri-Columbia 65212.
Boesman-Finkelstein
M
M
Finkelstein
R A
RA
McIntosh
M A
MA
D000595:Amino Acid Sequence; D001483:Base Sequence; D002772:Cholera Toxin / Q000235:genetics*; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D014734:Vibrio cholerae / Q000235:genetics*
D002772:Cholera Toxin