Changes of 6-keto PGF1 alpha concentration in plasma and vessel wall and TxB2 in plasma of whole-body irradiated rats in the early stage of irradiation.
The aim of this work was to monitor concentrations of prostanoids affecting platelet aggregation, namely prostacyclin and thromboxane A2, in the plasma and prostacyclin in the endothelium of vessel wall after whole-body irradiation of rats by the doses 5, 10, 50, 100 and 250 Gy (source 60Co) in the early stage of irradiation up to 24 hours. Stable metabolites of the prostanoids - 6-keto PGF1 alpha and TxB2 were determined by a RIA technique. The 6-keto PGF1 alpha plasma concentration increased after irradiation by the 50 Gy dose between 3rd and 24th hours and at all sampling times after the 100 and 250 Gy doses. A rise in prostanoid concentrations was noted after low doses of irradiation in the first sampling intervals only. The TxB2 level increased after the same doses (5 and 10 Gy) up to the 6th hour and then decreased. The thromboxane concentration increased after 50 Gy at the 90th minute, 12th and 24th hours, after 100 Gy at the 24th hour and after 250 Gy at the 90th minute, 3rd and 24th hours.
Navrátil
L
L
Institute of Biophysics and Nuclear Medicine, Faculty of General Medicine, Charles University, Prague, Czechoslovakia.
Pospiŝil
J
J
Blehová
Z
Z
D015121:6-Ketoprostaglandin F1 alpha / Q000097:blood*; D000818:Animals; D004730:Endothelium, Vascular / Q000737:chemistry*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013927:Thrombosis / Q000209:etiology; D013929:Thromboxane B2 / Q000097:blood*; D014916:Whole-Body Irradiation / Q000009:adverse effects
D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha