title: Molecular cloning of complementary DNAs encoding two cationic peroxidases from cultivated peanut cells.
abstract: We have isolated, cloned, and characterized two cDNAs corresponding to the mRNAs for cationic peroxidases synthesized by cultured peanut cells. The first clone was obtained from a phage lambda gt11 library screened with antibodies directed against the major secreted isozyme. Its predicted amino acid sequence, deduced from the 1228-base-pair (bp) cDNA, revealed a 22-amino acid signal peptide and a 294-amino acid mature protein (Mr, 31,228). The second clone was isolated from a lambda gt10 library screened with oligonucleotides corresponding to the regions for acid/base catalysis and the fifth ligand of heme. This cDNA (1344 bp) encodes a protein (330 amino acids) with a mature peptide of 307 residues (Mr, 32,954). The two peanut peroxidases are 46% homologous. The estimated gene copy numbers of these peroxidases might be close to 1 or 2 per haploid genome. A comparison of the amino acid sequence of these peanut peroxidases with other known isozymes shows two already known regions of homology (the region for acid/base catalysis and the fifth ligand of heme). Moreover, some new characteristics appeared such as a glycosylation site identical in five of the seven isozymes, a putative antigenic determinant common to all the isozymes, and a region of the highest homology. A secondary structure prediction showed that it corresponds to a 16-amino acid helix linked to the next one by a long stretch of beta strands and coils and might represent a critical structural element.
authors: Institut des Sciences Végétales, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
authors: D
authors: D
authors: Buffard
authors: C
authors: C
authors: Breda
authors: R B
authors: RB
authors: van Huystee
authors: O
authors: O
authors: Asemota
authors: M
authors: M
authors: Pierre
authors: D B
authors: DB
authors: Ha
authors: R
authors: R
authors: Esnault
mesh_terms: D000595:Amino Acid Sequence; D010367:Arachis / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015723:Gene Library; D005796:Genes; D008969:Molecular Sequence Data; D009841:Oligonucleotides; D010544:Peroxidases / Q000235:genetics*
chemical_list: D009841:Oligonucleotides; D004247:DNA; D010544:Peroxidases