Circulating glycoconjugates in CSF of meningioma patients.
This study investigated whether tumor-associated gangliosides or glycoproteins may be markers for meningiomas in CSF. The concentrations of a number of gangliosides and sulphatide were determined with specific monoclonal antibodies and cholera toxin B-subunit in a thin-layer chromatography overlay technique. As compared to a reference group of 10 individuals (mean age 50 +/- 14 years), the CSF samples from 7 meningioma patients (mean age 55 +/- 12 years) were found to contain significantly increased concentrations of ganglioside GD3 [II3(NeuAc)2-LacCer], one of the major gangliosides in meningioma tissue specimens. Sulphatide was significantly increased in 6 of 7 patients. Tumor-associated glycoproteins detected by the lectin Ricinus communis 1 in meningioma tissue specimens, but not in normal human brain tissue, were also found in the CSF of the meningioma patients. An early diagnosis of meningiomas might be achieved by the combined assay in CSF of ganglioside GD3 and glycoproteins with Ricinus communis lectin.
Davidsson
P
P
Department of Psychiatry, St. Jörgen Hospital, Hisings Backa, Sweden.
Fredman
P
P
von Holst
H
H
Wikstrand
C J
CJ
He
X
X
Bigner
D D
DD
Svennerholm
L
L
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000134:cerebrospinal fluid*; D005732:Gangliosides / Q000134:cerebrospinal fluid; D006001:Glycoconjugates / Q000134:cerebrospinal fluid*; D006017:Glycolipids / Q000134:cerebrospinal fluid; D006023:Glycoproteins / Q000134:cerebrospinal fluid; D006801:Humans; D008577:Meningeal Neoplasms / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D008579:Meningioma / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D013433:Sulfoglycosphingolipids / Q000134:cerebrospinal fluid
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D005732:Gangliosides; D006001:Glycoconjugates; D006017:Glycolipids; D006023:Glycoproteins; D013433:Sulfoglycosphingolipids