Optimization of 7 alpha-hydroxysteroid dehydrogenase production by Escherichia coli 080.
7 alpha-Hydroxysteroid dehydrogenase (EC 1.1.1.159) production by Escherichia coli strain 080 was highest when the organism was grown in brain heart infusion broth at pH 6.5 for 72-96 h with shaking at 37 degrees C. The oxygen consumption rate had a strong effect on the production of this constitutive enzyme. Glucose and lactose at 0.2-0.4%, detergents, and ethylenediaminetetra-acetic acid were found to increase the enzyme production.
Prabha
V
V
Department of Microbiology, Panjab University, Chandigarh, India.
Gupta
M
M
Gupta
K G
KG
D003470:Culture Media; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development; D006863:Hydrogen-Ion Concentration; D006913:Hydroxysteroid Dehydrogenases / Q000096:biosynthesis*; D010101:Oxygen Consumption; D013696:Temperature
D003470:Culture Media; D006913:Hydroxysteroid Dehydrogenases; C025376:7 alpha-hydroxysteroid dehydrogenase