Identification of novel hydroxy fatty acids in the barnacle Balanus balanoides.
The ability of the barnacle Balanus balanoides tissues to produce eicosanoid hatching factors from endogenous polyunsaturated fatty acids has been investigated. GC-MS analysis of an active HPLC fraction from the trihydroxy fatty acid band on TLC revealed the presence of a number of trihydroxy fatty acids and two compounds which were tentatively identified as chlorinated dihydroxy fatty acids. The identified trihydroxy fatty acids are 10,11,12-trihydroxy-5,8,14-eicosatrienoic acid, 10,11,12-trihydroxy-5,8,14,17-eicosatetraenoic acid, 13,14,15-trihydroxy-5,8,11,17-eicosatetraenoic acid and 12,13,14-trihydroxy-4,7,10,16,19-docosapentaenoic acid. The tentatively identified chlorinated dihydroxy fatty acids are 9-chloro- and/or 11-chloro-8,12-dihydroxyeicosatetraenoic acid. The formation of these compounds is evidence of lipoxygenase activities in Balanus balanoides and their identification will facilitate the understanding of the roles eicosanoids play in barnacle physiology, especially with regard to the larval hatching process.
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Marine Science Laboratories, University College of North Wales, Menai Bridge, Anglesey, U.K.
Holland
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Gibson
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Clayton
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Oldfield
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D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D015777:Eicosanoids / Q000302:isolation & purification*; D005231:Fatty Acids, Unsaturated / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006880:Hydroxy Acids / Q000302:isolation & purification*; D007202:Indicators and Reagents; D001468:Thoracica / Q000032:analysis*; D014020:Tissue Extracts / Q000302:isolation & purification
D015777:Eicosanoids; D005231:Fatty Acids, Unsaturated; D006880:Hydroxy Acids; D007202:Indicators and Reagents; D014020:Tissue Extracts