title: Stretch activates myosin light chain kinase in arterial smooth muscle.
abstract: Stretching of porcine carotid arterial muscle increased the phosphorylation of the 20 kDa myosin light chain from 0.23 to 0.68 mol [32P]phosphate/mol light chain, whereas stretching of phorbol dibutyrate treated muscle increased the phosphorylation from 0.30 to 0.91 mol/mol. Two-dimensional gel electrophoresis followed by two-dimensional tryptic phosphopeptide mapping was used to identify the enzyme involved in the stretch-induced phosphorylation. Quantitation of the [32P]phosphate content of the peptides revealed considerable light chain phosphorylation by protein kinase C only in the phorbol dibutyrate treated arterial muscle, whereas most of the light chain phosphorylation was attributable to myosin light chain kinase. Upon stretch of either the untreated or treated muscle, the total increment in [32P]phosphate incorporation into the light chain could be accounted for by peptides characteristic for myosin light chain kinase catalyzed phosphorylation, demonstrating that the stretch-induced phosphorylation is caused by this enzyme exclusively.
authors: Department of Physiology and Biophysics, College of Medicine, University of Illinois, Chicago 60612.
authors: K
authors: K
authors: Bárány
authors: A
authors: A
authors: Rokolya
authors: M
authors: M
authors: Bárány
mesh_terms: D000818:Animals; D002339:Carotid Arteries / Q000201:enzymology / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D004789:Enzyme Activation; D066298:In Vitro Techniques; D009126:Muscle Relaxation; D009131:Muscle, Smooth, Vascular / Q000201:enzymology / Q000502:physiology*; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism*; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D010710:Phosphates / Q000378:metabolism; D010750:Phosphoproteins / Q000302:isolation & purification; D010761:Phosphorus Radioisotopes; D013552:Swine
chemical_list: D010710:Phosphates; D010750:Phosphoproteins; D010761:Phosphorus Radioisotopes; D015240:Phorbol 12,13-Dibutyrate; D009219:Myosin-Light-Chain Kinase