title: The effects of shear stress on endothelial cell retention and function on expanded polytetrafluoroethylene.
abstract: We evaluated the adherence of indium 111-radiolabeled endothelial cells to fibronectin-treated expanded polytetrafluoroethylene surfaces exposed to high (437 s-1) vs low (218 s-1) shear and the influence of shear on prostacyclin production. Canine jugular vein factor VIII-positive endothelial cells in passages 3 through 6 were incubated with 111Indium-oxine, and labeled cells were seeded onto fibronectin-treated expanded polytetrafluoroethylene patches. Patches with confluent cells were exposed to shear in a Weissenberg rheogoniometer for intervals ranging up to 60 minutes. Percent endothelial cell retention was determined by gamma counting of patches and media and by histologic evaluation. Prostacyclin production (tritiated radioimmunoassay of 6-keto-prostaglandin F1 alpha) was assayed on perfusing media. Results showed no differences in 6-keto-prostaglandin F1 alpha production between shear rates or time periods. Endothelial cell retention did not differ between the shear rates. Rotational shear caused persistent cell loss over time in either high- or low-shear conditions. This persistent cell loss in response to steady rotational shear differs from that in response to identical rates of pulsatile linear shear in our laboratory where cell loss approached zero after 15 minutes.
authors: Department of Surgery, Loyola University Medical Center, Maywood, Ill. 60153.
authors: H P
authors: HP
authors: Greisler
authors: S
authors: S
authors: Johnson
authors: K
authors: K
authors: Joyce
authors: S
authors: S
authors: Henderson
authors: N M
authors: NM
authors: Patel
authors: T
authors: T
authors: Alkhamis
authors: R
authors: R
authors: Beissinger
authors: D U
authors: DU
authors: Kim
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001696:Biomechanical Phenomena; D002448:Cell Adhesion / Q000502:physiology*; D002478:Cells, Cultured; D004285:Dogs; D004730:Endothelium, Vascular / Q000502:physiology* / Q000648:ultrastructure; D011464:Epoprostenol / Q000096:biosynthesis; D007205:Indium Radioisotopes; D008855:Microscopy, Electron, Scanning; D011138:Polytetrafluoroethylene; D012399:Rotation; D013314:Stress, Mechanical; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D007205:Indium Radioisotopes; D011138:Polytetrafluoroethylene; D011464:Epoprostenol