Alveolysin, the thiol-activated toxin of Bacillus alvei, is homologous to listeriolysin O, perfringolysin O, pneumolysin, and streptolysin O and contains a single cysteine.
The gene coding for alveolysin, the thiol-activated toxin produced by Bacillus alvei, has been cloned by means of an oligonucleotide based on the known N-terminal sequence of the secreted protein. The complete nucleotide sequence of the gene has been determined. The deduced amino acid sequence of alveolysin shows that alveolysin shares homologies with listeriolysin O, perfringolysin O, pneumolysin, and streptolysin O. Alveolysin, like the other members of the family, contains a single cysteine in the conserved peptide sequence ECTGLA WEWWR.
Geoffroy
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C
Centre National de la Recherche Scientifique Unité Associée 557, Institut Pasteur, Paris, France.
Mengaud
J
J
Alouf
J E
JE
Cossart
P
P
D000595:Amino Acid Sequence; D001407:Bacillus / Q000235:genetics / Q000472:pathogenicity*; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000737:chemistry*; D001483:Base Sequence; D003001:Cloning, Molecular; D003545:Cysteine / Q000032:analysis; D006360:Heat-Shock Proteins / Q000235:genetics*; D006460:Hemolysin Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D009930:Organic Chemicals; D015183:Restriction Mapping; D013301:Streptolysins / Q000737:chemistry*
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; D009930:Organic Chemicals; D013301:Streptolysins; C032290:plY protein, Streptococcus pneumoniae; C025964:streptolysin O; C020452:Clostridium perfringens theta-toxin; C014963:alveolysin; D003545:Cysteine; C038705:hlyA protein, Listeria monocytogenes