title: Changes and localization of ovarian carbonyl reductase during pseudopregnancy and pregnancy in rats.
abstract: The present study investigates changes in the activity and enzyme content of ovarian carbonyl reductase (CR), which catalyzes the reduction of 9-keto and 15-ketoprostaglandins in rats during pseudopregnancy and pregnancy. The activity of ovarian CR decreased from the onset of pseudopregnancy and pregnancy, reaching 20-30% of the Day 1 value by Day 12 of pseudopregnancy and 50-60% of the Day 1 value by Day 14 of pregnancy. In the case of pregnant rats, the enzyme activity maintained a minimal level between Day 14 of pregnancy and Day 22 of parturition. An acute increase of the enzyme activity was found on the morning after parturition. The CR content in the ovary maintained a constant level from Day 1 to Day 12 of pseudopregnancy and to Day 18 of pregnancy. In pregnant rats, there was a gradual decrease after 18 days and then a surge during parturition. CR was primarily localized in interstitial gland cells and in theca interna cells but was not found in corpora lutea cells in the ovary during the estrous cycle. Additional immunostaining was also observed in corpora lutea cells during pseudopregnancy and pregnancy. The changes in ovarian CR activity, i.e. the rapid decrease with progressing pseudopregnancy and pregnancy, correlated with the increase in progesterone and the decrease in LH. These results indicate that rat ovarian CR may be regulated via the hypothalamo-pituitary-ovarian axis and may also be involved in luteal function.
authors: Department of Pharmacology and Toxicology, Tokyo College of Pharmacy, Hachioji, Japan.
authors: N
authors: N
authors: Iwata
authors: N
authors: N
authors: Inazu
authors: T
authors: T
authors: Satoh
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000449:Aldehyde Reductase; D000074408:Aldo-Keto Reductases; D000818:Animals; D015232:Dinoprostone / Q000031:analogs & derivatives / Q000378:metabolism; D005260:Female; D007986:Luteinizing Hormone / Q000378:metabolism; D010053:Ovary / Q000201:enzymology*; D011247:Pregnancy; D011270:Pregnancy, Animal; D011374:Progesterone / Q000096:biosynthesis; D011555:Pseudopregnancy / Q000201:enzymology*; D011725:Pyridines / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D011725:Pyridines; C025087:15-keto-13,14-dihydroprostaglandin E2; D011374:Progesterone; C066738:4-benzoylpyridine; D007986:Luteinizing Hormone; D000429:Alcohol Oxidoreductases; D000074408:Aldo-Keto Reductases; D000449:Aldehyde Reductase; D015232:Dinoprostone