title: Effects of etoposide (VP-16) on the survival and progression of cultured HeLa S3 cells through the cell cycle.
abstract: The survival of cultured HeLa S3 cells and their progression through the cell cycle after exposure to Etoposide (VP-16) were studied. The dose survival curve of exponentially growing cells was of a biphasic nature; the curve had an exponential slope starting from a small shoulder, and finally attained a plateau against increasing concentrations of the drug. Throughout the cell cycle, VP-16 exerted its main killing activity on cells in the late S and G2 phases, whereas cells in the G1 phase were resistant to the drug. By using a low concentration of VP-16, which allowed 85% of the treated cells to survive, it was found that the inhibition of the cell progression by the drug was greatest in the late S and G2 phases.
authors: Department of Surgery, School of Medicine, University of Chiba.
authors: S
authors: S
authors: Obata
authors: Y
authors: Y
authors: Yamaguchi
authors: T
authors: T
authors: Miyamoto
mesh_terms: D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005047:Etoposide / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D016196:S Phase; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000473:pathology
chemical_list: D005047:Etoposide