Recombination at ColE1 cer requires the Escherichia coli xerC gene product, a member of the lambda integrase family of site-specific recombinases.
Site-specific recombination at the plasmid ColE1 cer site requires the Escherichia coli chromosomal gene xerC. The xerC gene has been localized to the 85-min region of the E. coli chromosome, between cya and uvrD. The nucleotide sequences of the xerC gene and flanking regions have been determined. The xerC gene encodes a protein with a calculated molecular mass of 33.8 kDa. This protein has substantial sequence similarity to the lambda integrase family of site-specific recombinases and is probably the cer recombinase. The xerC gene is expressed as part of a multicistronic unit that includes the dapF gene and two other open reading frames.
Colloms
S D
SD
Institute of Genetics, University of Glasgow, Scotland.
Sykora
P
P
Szatmari
G
G
Sherratt
D J
DJ
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D003086:Bacteriocin Plasmids / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004254:DNA Nucleotidyltransferases / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D019426:Integrases; D008969:Molecular Sequence Data; D005810:Multigene Family; D009876:Operon; D011995:Recombination, Genetic; D015183:Restriction Mapping
D001426:Bacterial Proteins; D004254:DNA Nucleotidyltransferases; D019426:Integrases