Sequence of a sea urchin hsp70 gene and its 5' flanking region.
We report the nucleotide sequence of a 4470-bp fragment derived from a sea urchin genomic clone containing part of a heat-shock protein 70 (Hsp70)-encoding gene. This fragment, named hsp70 gene II, contains 1271 bp of the flanking region and 3299 bp of structural gene sequence interrupted by five introns and encoding the N-terminal 371 amino acids (aa) of the protein. The 5' flanking region contains a putative TATA element, two CCAAT boxes, four heat-shock consensus sequence elements (hse) and one consensus sequence for binding of Sp1. Remarkable homologies were observed for deduced aa sequence and intron-exon organization between hsp70 gene II and rat hsc73 gene.
La Rosa
M
M
Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo, Italy.
Sconzo
G
G
Giudice
G
G
Roccheri
M C
MC
Di Carlo
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016384:Consensus Sequence; D005091:Exons; D005796:Genes; D006360:Heat-Shock Proteins / Q000235:genetics*; D007438:Introns; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D012617:Sea Urchins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D006360:Heat-Shock Proteins