title: MHC-restricted antibody responses to Trichuris muris excretory/secretory (E/S) antigen.
abstract: Two panels of H-2 recombinant mice were used in a detailed serological study to analyse the role of H-2-linked genes in the control of the antibody response to excretory/secretory (E/S) antigens of Trichuris muris. An apparent H-2q (I-Aq) restriction on the early development of high levels of IgG1 antibody to E/S antigen was revealed by ELISA. No such restriction was demonstrated for the specific IgG2a response patterns. Recognition of two high molecular weight antigens (90-95 kDa, 105-110 kDa) by IgG antibodies was also shown to be almost exclusively H-2q restricted and may be related at least in part to the high antibody levels seen for H-2q strains of mice. Immune serum from resistant (B10.BRxB10.G) F1 hybrid mice (H-2q/k) containing high levels of IgG1 antibodies specific for T. muris E/S and IgG antibodies which recognized the 90-95 kDa and 105-110 kDa E/S antigens was effective in transferring protection to the non-responsive B10.BR mouse strain as seen on day 35 post-infection (p.i.). It is suggested that the IgG responses described for the generally very resistant H-2q mouse strains may contribute to, but not be an absolute requirement for, protective immunity, antibody-mediated damage facilitating a subsequent cellular attack in certain strains of mice.
authors: MRC Experimental Parasitology Unit, Department of Zoology, Nottingham University, University Park, UK.
authors: K J
authors: KJ
authors: Else
authors: D
authors: D
authors: Wakelin
authors: D L
authors: DL
authors: Wassom
authors: K M
authors: KM
authors: Hauda
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis*; D000947:Antigens, Helminth; D006183:H-2 Antigens / Q000235:genetics*; D006239:Haplotypes; D007116:Immunization, Passive; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011995:Recombination, Genetic; D014257:Trichuriasis / Q000235:genetics / Q000276:immunology; D014258:Trichuris / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D006183:H-2 Antigens; D007074:Immunoglobulin G