title: Immunohistochemical demonstration of Mycoplasma gallinarum and Mycoplasma gallinaceum in naturally infected hen oviducts.
abstract: Using indirect immunoperoxidase (IIP), peroxidase anti-peroxidase (PAP) and avidin biotin-peroxidase complex (ABC) immunohistochemical methods, Mycoplasma gallinarum and M gallinaceum antigens were demonstrated in ethanol-fixed paraffin-embedded oviduct sections from hens the eggs from which showed suboptimal hatchability. Specific immunoperoxidase staining was detected at the mucosa in the magnum portion of the oviduct. Optimal staining was achieved by applying the ABC method, though both IIP and PAP methods can also be used for diagnosis. Isolation and identification techniques gave similar results for the species of avian mycoplasmas involved.
authors: Departamento de Anatomia y Patológica, Facultad de Veterinaria, Cordoba, Spain.
authors: J
authors: J
authors: Martin de las Mulas
authors: A
authors: A
authors: Fernández
authors: M A
authors: MA
authors: Sierra
authors: J B
authors: JB
authors: Poveda
authors: J
authors: J
authors: Carranza
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D002645:Chickens; D005260:Female; D007106:Immune Sera / Q000276:immunology; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D009174:Mycoplasma / Q000276:immunology / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000382:microbiology / Q000662:veterinary*; D010057:Oviducts / Q000382:microbiology*; D011201:Poultry Diseases / Q000382:microbiology*
chemical_list: D007106:Immune Sera