The micronucleus assay in Anodonta cygnea for the detection of drinking water mutagenicity.
A micronucleus test in gill cells of the freshwater mussel Anodonta cygnea has been proposed for the detection of drinking water genotoxicity. Animals were exposed for 28 days to a drinking water sample and collected every week. Highly significant increases in spontaneous MN frequency were observed at each sampling, especially after 13 days of exposure. As positive control 2 doses of mytomicin C (MMC) were used (10(-8) and 10(-7) M). A second experiment was performed at a municipal waterworks in order to assess the role of water treatment processes in the production of mutagenic compounds. The most prevalent genotoxic effects were detected after chlorination (mean: 10.47% +/- 3.05, p less than 0.001).
Scarpato
R
R
Dipartimento di Scienze dell'Ambiente e del Territorio, Università di Pisa, Italy.
Migliore
L
L
Barale
R
R
D000818:Animals; D049872:Bivalvia / Q000235:genetics*; D002478:Cells, Cultured; D002713:Chlorine; D005618:Fresh Water; D015162:Micronucleus Tests; D009153:Mutagens / Q000032:analysis*; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000032:analysis*; D014881:Water Supply
D009153:Mutagens; D014874:Water Pollutants, Chemical; D002713:Chlorine