title: Characterization of a DNA binding protein of bacteriophage PRD1 involved in DNA replication.
abstract: Escherichia coli phage PRD1 protein P12, involved in PRD1 DNA replication in vivo, has been highly purified from E. coli cells harbouring a gene XII-containing plasmid. Protein P12 binds to single-stranded DNA as shown by gel retardation assays and nuclease protection experiments. Binding of protein P12 to single-stranded DNA increases about 14% the contour length of the DNA as revealed by electron microscopy. Binding to single-stranded DNA seems to be cooperative, and it is not sequence specific. Protein P12 also binds to double-stranded DNA although with an affinity 10 times lower than to single-stranded DNA. Using the in vitro phage phi 29 DNA replication system, it is shown that protein P12 stimulates the overall phi 29 DNA replication.
authors: Department of Genetics, University of Helsinki, Finland.
authors: T M
authors: TM
authors: Pakula
authors: J
authors: J
authors: Caldentey
authors: M
authors: M
authors: Serrano
authors: C
authors: C
authors: Gutierrez
authors: J M
authors: JM
authors: Hermoso
authors: M
authors: M
authors: Salas
authors: D H
authors: DH
authors: Bamford
mesh_terms: D003090:Coliphages / Q000187:drug effects / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects*; D004277:DNA, Single-Stranded / Q000378:metabolism; D004279:DNA, Viral / Q000096:biosynthesis*; D004268:DNA-Binding Proteins / Q000235:genetics*; D009690:Nucleic Acid Conformation; D011487:Protein Conformation; D014764:Viral Proteins / Q000494:pharmacology*
chemical_list: D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D014764:Viral Proteins; D004247:DNA