[3H]prazosin binding in the intermediolateral cell column and the effects of iontophoresed methoxamine on sympathetic preganglionic neuronal activity in the anaesthetized cat and rat.
The autoradiographic localization of [3H]prazosin (alpha 1-adrenoceptor ligand) binding sites was determined in cat spinal cord sections. High levels of [3H]prazosin binding were found in the intermediolateral cell column (IML) at thoracic and lumbar levels. The iontophoresis of the alpha 1-adrenoceptor agonist methoxamine onto sympathetic preganglionic neurones (SPNs) in anaesthetized cats and rats caused excitation of 8 cat SPNs and 13 rat SPNs. These results suggest an excitatory role for some of the catecholaminergic innervation of the IML.
Marks
S A
SA
Department of Physiology, Royal Free Hospital School of Medicine, London, U.K.
Stein
R D
RD
Dashwood
M R
MR
Gilbey
M P
MP
D000758:Anesthesia; D000818:Animals; D001345:Autoradiography; D002415:Cats; D005260:Female; D007478:Iontophoresis; D008297:Male; D008729:Methoxamine / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects*; D011224:Prazosin / Q000378:metabolism*; D051381:Rats; D013116:Spinal Cord / Q000166:cytology / Q000187:drug effects*; D013564:Sympathetic Nervous System / Q000166:cytology* / Q000187:drug effects
D008729:Methoxamine; D011224:Prazosin