In vitro kinetics of the oxidative reactivity of nitrate and nitrite in the rat erythrocyte.
Isolated rat erythrocytes were incubated in the presence of nitrate and nitrite. Glucose, lactate, reduced glutathione, methaemoglobin, malondialdehyde and Na+/K+ membrane exchange were investigated. Nitrite induced a strong methaemoglobinaemia and a net depletion of reduced glutathione in the intracellular medium associated with membrane lipid peroxidation. This oxidative reactivity induced by nitrate and nitrite altered the cell's ionic flux.
Batina
P
P
Université P. Sabatier, Institut de Physiologie, INSERM U-87, Toulouse, France.
Fritsch
P
P
de Saint Blanquat
G
G
Mitjavila
M T
MT
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D007773:Lactates / Q000096:biosynthesis; D008297:Male; D009566:Nitrates / Q000494:pharmacology*; D009573:Nitrites / Q000494:pharmacology*; D010084:Oxidation-Reduction; D011188:Potassium / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000378:metabolism
D007773:Lactates; D009566:Nitrates; D009573:Nitrites; D012964:Sodium; D005947:Glucose; D011188:Potassium