Intracellular accumulation of isoproterenol enhances the lactate production in the perfused rat heart.
Effects of intracellular accumulation of isoproterenol (ISO) on lactate production were examined in perfused rat heart. The lactate production during ISO perfusion in rat heart was increased and subsequent addition of an inhibitor of catechol-O-methyl transferase (COMT) further enhanced the production, and the enhanced production was significantly reduced by uptake2 inhibitor. The perfusion with ISO free-medium in the heart with high intracellular accumulation of ISO produced lactate more than that in the low intracellular accumulation. The present experiments demonstrated that the enhanced lactate production is accompanied by intracellular accumulation of ISO in the perfused rat heart, and suggested that the accumulated ISO may activate intracellular beta-adrenoceptors in the rat heart.
Kurahashi
K
K
Pharmacology Division, Faculty of Medicine, Kyoto University, Japan.
Akimoto
Y
Y
Inoue
M
M
Kosakai
K
K
Fujiwara
M
M
D000818:Animals; D006321:Heart / Q000502:physiology; D007424:Intracellular Fluid / Q000378:metabolism; D007545:Isoproterenol / Q000493:pharmacokinetics*; D007773:Lactates / Q000096:biosynthesis*; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000648:ultrastructure; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains
D007773:Lactates; D007545:Isoproterenol