Enzymatic synthesis of phosphatidylserine on small scale by use of a one-phase system.
A modification of the phospholipase D-catalyzed synthesis of phosphatidylserine is described, which allows the handling of small quantities of lipid without the need for an ether-water system. By using octylglucoside to disperse the lipid during the enzymatic conversion, it was possible to reduce the volume of the reaction mixture to 50-100 microliters. The amount of lipid that can be handled in such small volumes is in the order of micrograms. This facilitates the synthesis of phosphatidylserine from rare or expensive phosphatidylcholine species. The yield of phosphatidylserine is increased by replacing phospholipase D from cabbage by the enzyme from Streptomyces species.
Comfurius
P
P
Department of Biochemistry, University of Limburg, Maastricht, The Netherlands.
Bevers
E M
EM
Zwaal
R F
RF
D003902:Detergents; D010718:Phosphatidylserines / Q000096:biosynthesis*; D010739:Phospholipase D
D003902:Detergents; D010718:Phosphatidylserines; D010739:Phospholipase D