title: Cell proliferation in chorionic villi at different gestational ages, as analyzed by premature chromosome condensation.
abstract: The technique of premature chromosome condensation (PCC) was adapted to human first-trimester chorionic villi cells to analyze the cell-cycle kinetics of interphase chromatin. Uncultured cells of the cytotrophoblast (CT) and the mesenchymal core (MC) were obtained by a two-step digestion. PCC was induced by fusion of the chorionic interphase cells with mitotic Chinese hamster ovary or HeLa cells. Cells showing PCC in G1 (classes 1-6), S, and G2 were found. To analyze further the proliferation stages of chorionic G1 interphases, the proliferation potential index (PPI) of 34 placentae recovered between the 8th and 12th week of gestation was determined. The mean PPI found in the CT and MC cells ranged from 18% to 73%, values similar to those described for intensely proliferating tissues. The highest mean PPI value (73%) was observed in CT cells from placentae recovered at the 9th week of gestation, indicating a high specific proliferative activity of CT cells at this developmental stage.
authors: Department of Human Genetics, Medizinische Hochschule Hannover, FRG.
authors: G
authors: G
authors: Raabe
authors: K
authors: K
authors: Miller
mesh_terms: D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002824:Chorionic Villi / Q000502:physiology*; D002843:Chromatin / Q000378:metabolism; D002877:Chromosomes, Human / Q000648:ultrastructure*; D006224:Cricetinae; D005260:Female; D005865:Gestational Age; D006367:HeLa Cells; D006801:Humans; D007399:Interphase; D007700:Kinetics; D008648:Mesoderm / Q000166:cytology*; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D014327:Trophoblasts / Q000166:cytology*
chemical_list: D002843:Chromatin