Stereochemical features of 1,4-benzodiazepin-2-ones bound to human serum albumin: difference CD and UV studies.
The stereochemistry of an achiral (Diazepam) and two chiral (3-methyl and 3-succinyloxy substituted) 1,4-benzodiazepin-2-ones interacting with human serum albumin (HSA) has been investigated by making use of difference absorption (UV) and circular dichroism (CD) spectroscopies. Evidence is obtained for a higher affinity with HSA for one of the two possible conformations of the seven-membered benzodiazepine ring. The red shift revealed by the absorption difference spectrum between the free and the bound drug accounts for the CD difference spectra observed.
Bertucci
C
C
Dipartimento di Chimica e Chimica Industriale, Università di Pisa, Italy.
Domenici
E
E
Salvadori
P
P
D001570:Benzodiazepinones / Q000737:chemistry*; D002942:Circular Dichroism; D012709:Serum Albumin / Q000737:chemistry / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D001570:Benzodiazepinones; D012709:Serum Albumin