title: Modulation of hormone secretion in vitro by murine retrovirus.
abstract: In an attempt to elucidate the role of viruses in certain neuroendocrine disorders, we have demonstrated that infection of endocrine cells (GH-3 and Y-1) in vitro by moloney murine leukemia virus (M-MuLV) resulted in diminution of cell-specific secretory function, hormone secretion into culture. In GH-3 (rat anterior pituitary gland) active (initial) and persistent infection by M-MuLV resulted in approximately 80% reduction in prolactin and growth hormone secretion. The adrenal cortex tumor cell line (Y-1), when actively infected with the same virus, showed a transient increase in fluorogenic steroid secretion; however, on subsequent passages of infected cell cultures, steroid secretion was markedly reduced to about 10% of the uninfected Y-1 cells. The virus yield from M-MuLV-infected cultures of Y-1 and GH-3 cells produced a significantly lower amount of virus than the control NIH-3T3-infected cell cultures.
authors: Department of Microbiology, Howard University College of Medicine, Washington, D.C.
authors: Y A
authors: YA
authors: Nsiah
authors: F
authors: F
authors: Grissom
authors: W
authors: W
authors: Turner
mesh_terms: D000306:Adrenal Cortex Neoplasms / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D000818:Animals; D002455:Cell Division; D002460:Cell Line; D013006:Growth Hormone / Q000378:metabolism*; D008979:Moloney murine leukemia virus / Q000254:growth & development / Q000502:physiology*; D010903:Pituitary Gland, Anterior; D010911:Pituitary Neoplasms / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D011388:Prolactin / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D011388:Prolactin; D013006:Growth Hormone