Characterization and distribution of bombesin-like peptides in the rat brain and gastrointestinal tract.
Extracts of rat brain and gastrointestinal tract, analyzed by reverse-phase high-performance liquid chromatography and radioimmunoassay, contained two bombesin-like immunoreactivity peaks with similar retention times as porcine gastrin-releasing peptide (GRP) and its COOH-terminal decapeptide, neuromedin C or GRP(18-27). However, the GRP-like peptide peak did not elute with exactly the same retention time as porcine GRP. The highest concentration of bombesin-like immunoreactivity was found in extracts of antrum, whereas the lowest was found in whole brain. Neuromedin C was present at lower concentrations than the GRP in antrum, duodenum, and ileum, while similar amounts of each were found in brain.
Hernanz
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Servicio de Bioquímica, Hospital La Paz del Insalud, Madrid, Spain.
D000818:Animals; D001839:Bombesin / Q000031:analogs & derivatives* / Q000032:analysis; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004064:Digestive System / Q000737:chemistry*; D019886:Gastrin-Releasing Peptide; D008297:Male; D010455:Peptides / Q000032:analysis; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D010455:Peptides; D019886:Gastrin-Releasing Peptide; D001839:Bombesin