Possible methodologies for the detection and study of DNA sequence changes following mutagen exposure: magnetic enrichment in mutant DNA.
The restriction site mutation method described by Parry et al. (1990) for isolating mutant sequences directly from DNA is discussed and an additional enrichment step proposed using magnetic beads.
Bridges
B A
BA
MRC Cell Mutation Unit, University of Sussex, Falmer, Brighton, UK.
Carr
A M
AM
Lehmann
A R
AR
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000187:drug effects / Q000235:genetics*; D005821:Genetic Techniques; D008280:Magnetics; D016296:Mutagenesis; D009153:Mutagens / Q000494:pharmacology*; D016133:Polymerase Chain Reaction / Q000379:methods; D015183:Restriction Mapping
D009153:Mutagens; D004247:DNA