[Effects of indomethacin on 45Ca uptake and membrane fluidity of mitochondria and microsomes from rat liver].
We have reported previously that indomethacin-pretreatment could induce marked hepatic protection in rats. The present work reported effects of indomethacin on calcium regulation and membrane fluidity of mitochondria and microsomes of hepatocytes. The results showed that calcium uptake and membrane fluidity increased significantly in mitochondria and microsomes of hepatocytes isolated from the rats pretreated with indomethacin in vivo as compared with that from the control; adding indomethacin directly to isolated mitochondria and microsomes from normal rats in vitro, on the contrary, decreased membrane fluidity. These changes may be related to the protective effect of indomethacin.
Shen
J
J
Department of Physiology, Beijing Medical University.
Zhang
X J
XJ
Nie
S Q
SQ
Wang
C C
CC
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002251:Carbon Tetrachloride; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism; D007213:Indomethacin / Q000494:pharmacology*; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects*; D008862:Microsomes, Liver / Q000187:drug effects*; D008930:Mitochondria, Liver / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains
D002251:Carbon Tetrachloride; D002118:Calcium; D007213:Indomethacin