[Recent development of determination methods for adrenocortical hormones and catecholamines].
Newly developed resins and high-performance liquid chromatography (HPLC) with column switching technique have enabled on-line solid-phase extraction, purification and derivatization for highly sensitive determination in recently developed determination methods for serum cortisol, urinary 17-ketosteroids (17-KS), urinary conjugated 17-KS fractions and urinary free catecholamines (CA). These methods are (1) determination of serum cortisol by HPLC with a BSA-ODS column (TSK) and serum sample direct injection, (2) determination of fluorescently derivatized serum cortisol with sulfuric acid and ethanol by column switching HPLC, (3) determination of urinary 17-KS with no need of previous hydrolysis of the conjugated forms, (4) HPLC of conjugated 17-KS fluorescently prelabelled with dansyl hydrazine, (5) HPLC of urinary free catecholamines (CA) with on-line extraction using a cation exchange column and on-line fluorescent derivatization with 1,2-diphenylethylenediamine, (6) ion pair HPLC of urinary free CA with on-line extraction using a hard boric acid gel column and postcolumn trihydroxyindole derivatization, and (7) HPLC of urinary free CA with on-line extraction and solid-phase derivatization using a boric acid gel column, o-phthalaldehyde and 2-mercaptoethanol, have achieved no previous or simple sample extraction, more rapid, accurate and higher sensitive determination comparison with ordinary methods for these substances and furthermore, some methods have enabled fully automated analysis.
Nozaki
O
O
Department of Clinical Pathology, Kinki University School of Medicine, Osaka-Sayama.
Ohba
Y
Y
D015068:17-Ketosteroids / Q000652:urine*; D002395:Catecholamines / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D006854:Hydrocortisone / Q000097:blood*
D015068:17-Ketosteroids; D002395:Catecholamines; D006854:Hydrocortisone