title: Inhibitors directed to binding domains in neutrophil elastase.
abstract: Human neutrophil elastase (HNE) can be inhibited by unsaturated fatty acids, including oleic acid [Ashe, B. M., & Zimmerman, M. (1977) Biochem. Biophys. Res. Commun. 75, 194-199; Cook, L., & Ternai, B. (1988) Biol. Chem. Hoppe-Seyler 369, 627-631], but is not affected by saturated fatty acids. We have shown that the interaction of oleic acid with HNE can be characterized by two apparent inhibitory modes: a high-affinity mode (Ki = 48 +/- 3 nM), resulting in partial noncompetitive inhibition (87% residual activity), and a competitive inhibitory mode of lower affinity (Ki = 16 +/- 1 microM). Binding of oleate in the high-affinity mode induces a blue shift in the endogenous fluorescence arising from the tryptophan residues in HNE. This shift is maximal in the presence of 1 microM oleate; higher concentrations of fatty acid have no further effect on the fluorescence spectrum. The negatively charged fluorescent ester of oleic acid and hydroxypyrenetrisulfonate (HPTSoleate) interacts with HNE at an apparent single site (Ki = 44 +/- 3 nM), resulting in competitive inhibition. A blue shift in the emission maximum of the pyrene fluorescence at 410 nm and a decrease in the ratio of the intensities of the maximum at 388 and 410 nm indicate that upon binding to HNE the environment of the pyrene ring in HPTSoleate becomes more hydrophobic.(ABSTRACT TRUNCATED AT 250 WORDS)
authors: Department of Biochemistry and Cell Biology, State University of New York, Stony Brook 11794.
authors: S C
authors: SC
authors: Tyagi
authors: S R
authors: SR
authors: Simon
mesh_terms: D001120:Arginine; D001665:Binding Sites; D006037:Glyoxal / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D019272:Leukocyte Elastase; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism* / Q000494:pharmacology; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D011721:Pyrenes / Q000494:pharmacology; D014364:Tryptophan
chemical_list: D009829:Oleic Acids; D011721:Pyrenes; C066961:pyrene glyoxal; D019301:Oleic Acid; D006037:Glyoxal; D014364:Tryptophan; D001120:Arginine; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase