A rapid procedure for eliminating chromatofocusing buffer and concentrating minor active subforms of mitochondrial malate dehydrogenase.
Mitochondrial malate dehydrogenase from several sources contains different molecular forms whose origin is still under discussion. Separation of these subforms has been achieved by chromatofocusing. A simple and rapid method, based on 5' AMP Sepharose chromatography, has been developed to concentrate mitochondrial malate dehydrogenase subforms and simultaneously remove chromatofocusing buffer.
Gelpí
J L
JL
Department de Bioqúimica i Fisiologia, Facultat de Química, Universitat de Barcelona, Spain.
Gracia
V
V
Imperial
S
S
Mazo
A
A
Cortés
A
A
D000818:Animals; D002021:Buffers; D002645:Chickens; D002846:Chromatography, Affinity / Q000379:methods*; D007525:Isoelectric Focusing; D008291:Malate Dehydrogenase / Q000302:isolation & purification* / Q000378:metabolism; D008930:Mitochondria, Liver / Q000201:enzymology
D002021:Buffers; D008291:Malate Dehydrogenase