An inhibitor of protein synthesis initiation from Alhagi kirgisorum S.
Polyproanthocyanidin--a plant phenolic compound from Alhagi kirgisorum S. effectively inhibited protein synthesis in rabbit reticulocyte and wheat germ cell-free systems. Poly-proanthocyanidin inhibited translation only at the level of initiation and not at the elongation level and aminoacylation of tRNA. The inhibitory effect of the phenolic compound is due to the blockage of the ternary complex formation of eIF-2 with GTP and initiator Met-tRNA.
Smailov
S K
SK
Ajtkhozhin Institute of Molecular Biology and Biochemistry, Kazakh Academy of Sciences, Alma-Ata, USSR.
Mukhamedzhanov
B G
BG
Lee
A V
AV
Iskakov
B K
BK
Denisenko
O N
ON
D000818:Animals; D015852:Eukaryotic Initiation Factor-2 / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D066298:In Vitro Techniques; D010442:Peptide Chain Initiation, Translational / Q000187:drug effects*; D010636:Phenols / Q000494:pharmacology*; D010944:Plants / Q000032:analysis; D012358:RNA, Transfer, Met / Q000378:metabolism; D011817:Rabbits; D012156:Reticulocytes; D012270:Ribosomes / Q000378:metabolism
D015852:Eukaryotic Initiation Factor-2; D010636:Phenols; D012358:RNA, Transfer, Met; C066697:polyproanthocyanidin; D006160:Guanosine Triphosphate