Development of micropatterning technology for cultured cells.
The manipulation of regional cell adhesiveness by surface design could provide micropatterned cell culturing. Based on the photoreactive chemistry of a phenylazide group, a novel surface micropatterning technology for cultured cells was successfully developed. The principle is as follows: 1) a photoreactive hydrophilic co-polymer with phenylazide was cast on a hydrophobic matrix surface, 2) a photoreactive hydrophobic co-polymer was cast on a hydrophilic matrix; 3) a photomask with a given pattern was tightly placed on the cast film; and 4) after UV irradiation and subsequent washing, bovine endothelial cells (ECs) were seeded and cultured. ECs adhered and grew only on nonhydrophilic regions, eventually resulting in micropatterning of ECs. The micropatterns of cultured ECs prepared by 1) and 2) were negative- and positive-type patterns to that of the photomask used, respectively.
Matsuda
T
T
Department of Bioengineering, National Cardiovascular Center, Osaka, Japan.
Inoue
K
K
Sugawara
T
T
D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology*; D002478:Cells, Cultured; D003470:Culture Media; D003584:Cytological Techniques; D004730:Endothelium, Vascular / Q000166:cytology*; D010777:Photochemistry; D011108:Polymers
D003470:Culture Media; D011108:Polymers