title: Aerodynamic evaluation of crystalloid and colloid flush perfusion for lung preservation.
abstract: To assess the effectiveness of pulmonary perfusion we evaluated the lung mechanics of 36 canine lungs in an isolated perfused working lung (IPWL) model. Four groups of lungs (n = 9 each) were preserved by pulmonary artery flushing with either high-potassium colloid (UW), high-potassium crystalloid (EuroCollins', EC), low-potassium crystalloid control (lactate), or low-potassium substrate-enhanced crystalloid (RPMI) followed by 130 +/- 10 min of cold storage. Ventilation remained constant (TV 10 ml/kg at 14 breaths/min with 5 cm H2O PEEP). Assessed data included lung resistance (R), timed expiratory volume (EV0.3 sec as %TV), lung compliance (C), elastic work (Wel), and flow-resistive work (Wres). Immediately following storage, R and Wel were similar for all groups (16 +/- 3 cm H2O/liter/sec and 149 +/- 18 gm/min). UW preserved lungs were less compliant (1.5 +/- 0.1 X 10(-2) liter/cm H2O) and required more inspiratory work (Wres 5.8 +/- 0.8 gm/min) compared to the low-potassium crystalloid (Lactate) group (2.0 +/- 0.1 X 10(-2) liter/cm H2O and 3.4 +/- 0.6 gm/min, respectively, P less than 0.05). For 3 hr of reperfusion, crystalloid lungs showed no significant change in R, C, Wel, or Wres. In contrast, R of the UW group increased significantly to 32 +/- 5 and 40 +/- 8 cmH2O/liter/sec at 1 and 3 hr, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
authors: Northwestern University Medical School, Department of Surgery, Chicago, Illinois 60611-3008.
authors: J
authors: J
authors: LoCicero
authors: M
authors: M
authors: Massad
authors: J
authors: J
authors: Matano
authors: F
authors: F
authors: Khasho
authors: R
authors: R
authors: Greene
mesh_terms: D000818:Animals; D003102:Colloids; D000077324:Crystalloid Solutions; D004285:Dogs; D005069:Evaluation Studies as Topic; D007552:Isotonic Solutions; D008168:Lung / Q000502:physiology; D008170:Lung Compliance; D010477:Perfusion; D010952:Plasma Substitutes; D011309:Preservation, Biological / Q000379:methods*; D015424:Reperfusion; D012121:Respiration, Artificial
chemical_list: D003102:Colloids; D000077324:Crystalloid Solutions; D007552:Isotonic Solutions; D010952:Plasma Substitutes