[A DNA study of rat liver oligonucleosomes enriched by transcriptionally active genes during induction due to the administration of an amino acid mixture].
A highly active fraction of rat liver oligonucleosome DNA has been isolated and studied by means of thermal denaturation after induction by amino acid mixture or hydrocortisone. A considerable redistribution of DNA content has been shown in sucrose gradient fractions during these forms of induction. The changes are revealed in melting temperature, differential melting profile of DNA, isolated from actively transcribed chromatine fractions. Analysis of melting profiles shows changes of GC content of oligonucleosome DNA, suggesting that there are differences in activation during two studied forms of induction.
Vardevanian
P O
PO
Davtian
A M
AM
Tiratsuian
S G
SG
Vardevanian
A O
AO
D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002843:Chromatin / Q000737:chemistry / Q000187:drug effects; D004247:DNA / Q000737:chemistry / Q000187:drug effects* / Q000302:isolation & purification; D006358:Hot Temperature; D006854:Hydrocortisone / Q000494:pharmacology; D008099:Liver / Q000737:chemistry / Q000187:drug effects*; D008297:Male; D009691:Nucleic Acid Denaturation / Q000187:drug effects; D009707:Nucleosomes / Q000737:chemistry / Q000187:drug effects*; D051381:Rats; D014158:Transcription, Genetic / Q000187:drug effects*
D000596:Amino Acids; D002843:Chromatin; D009707:Nucleosomes; D004247:DNA; D006854:Hydrocortisone
Izuchenie DNK oligonukleosom pecheni krys, obogashchennykh transkriptsionno aktivnymi genami, pri induktsii, vyzvannoĭ vvedeniem smesi aminokislot.