title: Activity measurement of lysosomal cysteine proteinases, cathepsins B, H and L, in crude tissue extracts, and their relation to the fractional rate of protein degradation.
abstract: 1. The fractional rate of protein degradation was measured in vivo and compared with the lysosomal enzyme activities of several rat tissues. Good agreement was observed between them. 2. A specific method for determining lysosomal cysteine-dependent cathepsins, B, L and H, was demonstrated.
authors: Department of Agricultural Chemistry, Tokyo University of Agriculture and Technology, Japan.
authors: R
authors: R
authors: Funabiki
authors: K
authors: K
authors: Yagasaki
authors: C
authors: C
authors: Shirakawa
authors: H
authors: H
authors: Sugita
authors: S
authors: S
authors: Ishiura
mesh_terms: D000818:Animals; D002403:Cathepsins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism*; D008297:Male; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity
chemical_list: D011506:Proteins; D002403:Cathepsins