[Study on the effect of various conditions and age to blood platelet aggregation test].
The result of the platelet aggregation test was affected by various conditions. We performed the platelet aggregation test by changing different conditions, including: 1. adding different anticoagulants 2. interval between sampling and standing 3. platelet count 4. the concentrations of different reagents (ADP, Epinephrine and Collagen) respectively and the aggregation rate of blood platelet were compared among different age groups. The best condition for platelet aggregation rate was obtained as follows: 1) Citric acid of 3.8% is the best anticoagulant. 2) Test should be completed within 4 hours upon sample collection. 3) The optimal incubation temperature is between 25 degrees to 37 degrees C. 4) Platelet count of 2 to 4 x 10(5)/microliters gives the most constant result. 5) The optimal concentration for working solution of ADP, collagen and Epinephrine are 5.0 microns, 5.0 micrograms/ml and 50 microM respectively. 6) The aggregation rate of blood platelet increases with age.
Lin
H C
HC
School of Technology for Medical Sciences, Kaohsiung Medical College, Taiwan, Republic of China.
Liu
H W
HW
Lin
S F
SF
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000328:Adult; D000367:Age Factors; D000368:Aged; D003094:Collagen / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D006801:Humans; D008875:Middle Aged; D010974:Platelet Aggregation / Q000187:drug effects; D010976:Platelet Count; D013696:Temperature; D013997:Time Factors
D000244:Adenosine Diphosphate; D003094:Collagen; D004837:Epinephrine