title: Interaction of puromycin with acceptor site of human placenta 80 S ribosomes.
abstract: The complex N-AcPhe-tRNA(Phe).poly(U).80 S ribosome from human placenta was treated with puromycin taken in various concentrations. Based on the kinetic data of N-acetylphenylalanyl-puromycin formation, the association constant of puromycin with the acceptor site of the ribosome was estimated to be (3.96 +/- 0.84) x 10(4) M-1 at 37 degrees C.
authors: Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences, Novosibirsk.
authors: D M
authors: DM
authors: Graifer
authors: O S
authors: OS
authors: Fedorova
authors: G G
authors: GG
authors: Karpova
mesh_terms: D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D010920:Placenta; D011691:Puromycin / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*
chemical_list: D011691:Puromycin