title: Critical periods to ethanol exposure during early neuroembryogenesis in the chick embryo: cholinergic neurons.
abstract: The acute and chronic effects of ethanol on cholinergic neuronal expression were studied in the chick embryo during early neuroembryogenesis using choline acetyltransferase (ChAT) activity as a cholinergic marker. Ethanol administered to embryos in ovo on day 1 (E1) produced a 30% decrease in ChAT activity, while ethanol administration on day 3 elicited no significant change. Similar effects were produced by ethanol on ChAT activity in the spinal cord. The decrease in ChAT activity in both brain and spinal cord was not accompanied by a significant change in protein content. Of significance were our findings with chronic ethanol treatment: in embryos treated from E1 to E5 and sacrificed at E6, ChAT activity was decreased. In contrast, in embryos treated similarly but sacrificed at E8 ChAT activity was increased. These findings establish that the critical period of cholinergic neuronal sensitivity to ethanol is confined to E. Moreover, the increase in ChAT activity observed after chronic ethanol treatment indicates that the developing neurons have the capability to adapt to ethanol. This apparent adaptation results in overcompensation, as reflected by the increase in ChAT activity. Whether this overcompensation is at the expanse of another neuronal population remains to be investigated.
authors: Department of Pharmacology, University of Colorado School of Medicine, Denver 80262.
authors: C
authors: C
authors: Brodie
authors: A
authors: A
authors: Vernadakis
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000196:embryology* / Q000201:enzymology; D002642:Chick Embryo; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D012016:Reference Values; D013116:Spinal Cord / Q000187:drug effects / Q000196:embryology* / Q000201:enzymology
chemical_list: D000431:Ethanol; D002795:Choline O-Acetyltransferase