Sulphation of hirudin in BHK cells.
Hirudin, a thrombin inhibitor of the leech, was expressed in BHK cells; the alpha 1-antitrypsin signal peptide was used to direct secretion into the culture medium. The recombinant hirudin so produced inhibited thrombin and was shown by labelling experiments with [35S]sulphate to have been posttranslationally modified.
Skern
T
T
Transgene SA, Strasbourg, France.
Bischoff
R
R
Jallat
S
S
Dott
K
K
Ali-Hadji
D
D
Clesse
D
D
Kieny
M P
MP
Courtney
M
M
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D006224:Cricetinae; D004252:DNA Mutational Analysis; D015870:Gene Expression; D006629:Hirudins / Q000378:metabolism*; D066298:In Vitro Techniques; D007668:Kidney; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals; D011994:Recombinant Proteins / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism*; D013917:Thrombin / Q000037:antagonists & inhibitors
D006629:Hirudins; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D013431:Sulfates; D013917:Thrombin