Lectin-binding sites in the anterior segment of the human eye.
The anterior segment of the human eye was screened for differences in the lectin-binding patterns of Con A, PNA, GS-I, WGA, SBA, DBA, and UEA-I to enable cell typing for cell-culture purposes. An immunohistochemical technique combining an indirect antibody-lectin method with the avidin-biotin system was used. Con A and WGA were bound by all cells except the conjunctival goblet cells. UEA-I was exclusively bound by both vascular endothelial cells and some corneal and conjunctival epithelial cells. The binding of GS-I, PNA, SBA, and DBA showed an uneven pattern and differed among the cases investigated. The reasons for these differences are not clear. Our results indicate that the usefulness of lectins for cell-typing purposes is restricted and must be determined for every case.
Rittig
M
M
Anatomisches Institut, Universität Erlangen-Nürnberg, Federal Republic of Germany.
Brigel
C
C
Lütjen-Drecoll
E
E
D000368:Aged; D000369:Aged, 80 and over; D000869:Anterior Eye Segment / Q000378:metabolism*; D002924:Ciliary Body / Q000378:metabolism; D003228:Conjunctiva / Q000378:metabolism; D003315:Cornea / Q000378:metabolism; D004730:Endothelium, Vascular / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007498:Iris / Q000378:metabolism; D037102:Lectins / Q000378:metabolism*; D008297:Male
D037102:Lectins