In vitro effect of plasma during phenylhydrazine-induced erythrocyte oxidative damage.
Erythrocytes were incubated for 60 min either in plasma or phosphate-buffered saline containing 10 mM phenylhydrazine hydrochloride. Plasma prevented the decrease in membrane fluidity observed in saline-phenylhydrazine incubated erythrocytes but these cells showed decreases in both filterability and active extrusion of Na+ that were nearly 100% lower than those found in erythrocytes incubated in plasma-phenyl-hydrazine. Also erythrocyte lipid peroxidation, as measured as thiobarbituric acid reactive products, was 100% higher in the presence of plasma. These results suggest that plasma could play "in vivo" an active role during oxidant-induced erythrocyte damage, contributing significantly to the hemolytic effects of oxidation.
Lucio Cazana
F J
FJ
Department of Physiology and Pharmacology, University of Alcala de Henares, Madrid, Spain.
Diez Marques
M L
ML
Jimenez
A
A
Rodriguez Puyol
M
M
D000818:Animals; D002470:Cell Survival; D004912:Erythrocytes / Q000187:drug effects*; D066298:In Vitro Techniques; D015227:Lipid Peroxidation; D008297:Male; D008560:Membrane Fluidity; D010659:Phenylhydrazines / Q000097:blood / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013858:Thiobarbiturates / Q000097:blood
D010659:Phenylhydrazines; D013858:Thiobarbiturates; C030299:phenylhydrazine