Isolation and characterization of levansucrase-encoding gene from Bacillus amyloliquefaciens.
The gene encoding levansucrase (LVS) from Bacillus amyloliquefaciens (sacB[BamP]) was isolated, sequenced and expressed in Bacillus subtilis. Analysis of the nucleotide sequence of sacB[BamP] reveals extensive homology with that of the B. subtilis LVS-encoding gene in the promoter and coding region. The sacB[BamP] gene cloned in a multicopy plasmid is induced by sucrose in B. subtilis.
Tang
L B
LB
Central Research and Development Division, E.I. duPont de Nemours Company, Wilmington, DE 19880-0228.
Lenstra
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R
Borchert
T V
TV
Nagarajan
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V
D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D015870:Gene Expression; D005798:Genes, Bacterial; D005809:Genes, Regulator; D006602:Hexosyltransferases / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D006602:Hexosyltransferases; C021513:levansucrase