[Effect of Chinese medicinal herbs on sperm membrane of infertile male].
The authors carried on the quantitative analysis of fluorescence polarization degree and fluorescent intensity on wheat germ agglutinin (WGA) receptor in sperm membrane surface and protein macromolecule 1-Anilinonaph thalene-8-suphonic acid salt (1,8-ANS) of sperm membrane to the patients with infertility and adult male with normal fertility, with the method of ultraviolet microspectrofluorometer (SMP, type: 05, made in West Germany). The results were as follows: the patient's WGA receptor in sperm membrane surface was decreased (P less than 0.001), and the 1, 8-ANS of fluorescent intensity in hydrophobic area of protein macromolecule of sperm membrane was increased (P less than 0.05). After the treatment of Shenjing Zhongzitang, WGA receptor was increased obviously and 1, 8-ANS of fluorescent intensity was changed to be almost normal. It showed that the Chinese medicinal herbs mentioned above have certain influence upon the constitution of WGA receptor and protein macromolecule of sperm membrane.
Liu
X D
XD
Shandong Institute of Traditional Chinese Medicine and Materia Medica, Jinan.
D000817:Anilino Naphthalenesulfonates / Q000378:metabolism; D002462:Cell Membrane; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D006801:Humans; D007248:Infertility, Male / Q000188:drug therapy*; D008297:Male; D011975:Receptors, Mitogen / Q000378:metabolism; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism
D000817:Anilino Naphthalenesulfonates; D004365:Drugs, Chinese Herbal; D011975:Receptors, Mitogen; C027503:wheat germ agglutinin receptor; C027132:1-anilino-8-naphthalenesulfonate