Effects of nutritional folate deficiency on the adhesive properties of murine melanoma cells.
Folate deficient murine B16 melanoma cells adhered more rapidly and in higher percentages to plastic plates or dishes coated with laminin or fibronectin than folate replete cells. These changes in the adhesive properties of murine melanoma cells induced by nutritional folate deficiency were not mediated by changes in cell size, proliferative capacity or cell cycle distribution. While melanoma cells served as a suitable surface for prothrombinase complex formation, folate deficiency did not alter this membrane function, suggesting that the membrane changes associated with folate deficiency are relatively specific.
Branda
R F
RF
Department of Medicine, University of Vermont, Burlington 05405.
Tracy
P B
PB
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism / Q000502:physiology; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D005353:Fibronectins; D005494:Folic Acid Deficiency / Q000378:metabolism / Q000473:pathology*; D007797:Laminin; D008546:Melanoma, Experimental / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D051379:Mice; D010969:Plastics; D013997:Time Factors; D014407:Tumor Cells, Cultured
D003470:Culture Media; D005353:Fibronectins; D007797:Laminin; D010969:Plastics