Cytogenetic analysis of human solid tumors by in situ hybridization with a set of 12 chromosome-specific DNA probes.
We have applied fluorescent in situ hybridization (FISH) to assess the presence of numerical chromosome aberrations in fresh specimens of human solid tumors of varying histology. For this purpose, a set of 12 biotinylated chromosome-specific, repetitive alpha-satellite DNA probes (for chromosomes 1, 6, 7, 9, 10, 11, 15, 16, 17, 18, X and Y) were hybridized directly to isolated interphase nuclei. Utilizing this approach, we found numerical chromosome changes in all tumors. FISH ploidy profiles were in accordance with flow cytometric DNA histograms of these tumor cells.
van Dekken
H
H
Department of Pathology, Memorial Sloan-Kettering Cancer Center, New York NY.
Pizzolo
J G
JG
Reuter
V E
VE
Melamed
M R
MR
D002869:Chromosome Aberrations; D002877:Chromosomes, Human; D015342:DNA Probes; D004276:DNA, Satellite; D004407:Dysgerminoma / Q000235:genetics; D005453:Fluorescence; D006801:Humans; D007621:Karyotyping; D008297:Male; D009369:Neoplasms / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010190:Pancreatic Neoplasms / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D012509:Sarcoma / Q000235:genetics
D015342:DNA Probes; D004276:DNA, Satellite