Preparation of a latex reagent for the detection of anti-Staphylococcus aureus ribitol teichoic acid antibodies.
Purified S. aureus ribitol teichoic acid was covalently bound to carboxylated latex particles. The immunological properties of the polysaccharide antigen were preserved. The reagent obtained was used for the quantification of anti-ribitol teichoic acid antibodies by means of a direct and rapid agglutination test carried out on a slide. There was good correlation between the preliminary results of this test and those obtained with counter-immunoelectrophoresis (CIE). The method is faster and more sensitive than CIE.
de Montclos
M
M
Bacteriology Laboratory, Université Claude Bernard Lyon I, Faculté de Médecine Lyon-Sud, Pierre-Benite, France.
Flandrois
J P
JP
D000907:Antibodies, Bacterial / Q000032:analysis*; D003378:Counterimmunoelectrophoresis; D006801:Humans; D007840:Latex; D007841:Latex Fixation Tests; D008863:Microspheres; D015203:Reproducibility of Results; D013211:Staphylococcus aureus / Q000276:immunology*; D013682:Teichoic Acids / Q000276:immunology*
D000907:Antibodies, Bacterial; D007840:Latex; D013682:Teichoic Acids; C044340:ribitol teichoic acid