Base sequences of highly repetitive components in nuclear DNAs from rat liver and rat-ascites hepatoma.
A 370-bp highly repetitive component in each of the nuclear DNAs from rat liver (RL) and rat-ascites hepatoma (AH) was isolated by HindIII digestion and cloned in pUC9. Ten of the resulting clones were arbitrarily selected and sequenced. Heterogeneity of size was found in 7 of the RL clones (366-369 bp), but in only 2 of the AH clones (369 bp). The sequence homology was 64.6% among the RL clones; 80.3% among the AH clones. The base compositions were AT-rich, ranging from 61.1% to 64.7%. Many A and/or T runs consisting of 2-5 bases were interspersed throughout each sequence. The restriction sites reported previously, EcoRI, HaeIII, HindIII, HinfI and HphI sites, were confirmed in almost all of the clones. In the present experiment, 12 kinds of the sites were further found in both RL and AH clones.
Ikeda
Y
Y
Cell Biology Division, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
Nakamura
K
K
Iwakami
N
N
Hibino
Y
Y
Sugano
N
N
D000818:Animals; D001201:Ascites / Q000473:pathology; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism / Q000502:physiology; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000378:metabolism; D004273:DNA, Neoplasm / Q000235:genetics* / Q000378:metabolism; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism; D015247:Deoxyribonuclease HindIII / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D008114:Liver Neoplasms, Experimental / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D051381:Rats
D004273:DNA, Neoplasm; D009687:Nuclear Proteins; D004247:DNA; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII