Production and characteristics of the exocellular polysaccharide of a mutant Mycobacterium strain.
Mutant strains of Mycobacterium sp. V-649 producing highly mucous colonies on a solid cultivation medium were prepared after treatment with N-methyl-N'-nitro-N-nitrosoguanidine and production of the exocellular polysaccharide was tested. The strains were cultivated in media with suitable sugar sources under submerged conditions. It was found that Mycobacterium sp. V-649/15 produces a maximum of 15-19% polymer after a 5-6-d cultivation. Gas chromatography indicated that the exocellular polysaccharide produced by this strain is of glucan type.
Konícek
J
J
Institute of Microbiology, Czechoslovak Academy of Sciences, Prague.
Daraselia
G Y
GY
Konícková-Radochová
M
M
Lasík
J
J
Wurst
M
M
Koza
T
T
D002849:Chromatography, Gas; D003470:Culture Media; D009154:Mutation; D009161:Mycobacterium / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000737:chemistry
D003470:Culture Media; D011135:Polysaccharides, Bacterial