Effects of cadmium on the energy metabolism of isolated hepatocytes: its relationship with the nonviability of isolated hepatocytes caused by cadmium.
The effects of cadmium on the energy metabolism of mitochondria were studied with isolated hepatocytes and rat liver mitochondria. It was found that cadmium inhibited the respiration of both isolated hepatocytes and mitochondria and decreased the ATP content of isolated hepatocytes. This inhibition of energy metabolism of mitochondria was highly related to the nonviability of isolated hepatocytes caused by cadmium. The site of electron transport of the mitochondrial respiratory chain blocked by cadmium was located between cytochrome b and flavo-proteins. The uncoupling effects of mitochondrial oxidative phosphorylation caused by cadmium may have resulted from changes in the fluidity and permeability of the mitochondrial membrane.
Liu
R M
RM
Department of Environmental Toxicology, Tongji Medical University, Wuhan, Hubei Province, China.
Liun
Y G
YG
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002104:Cadmium / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D002463:Cell Membrane Permeability / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003580:Cytochromes / Q000187:drug effects; D004734:Energy Metabolism / Q000187:drug effects*; D005260:Female; D005969:Glutamate Dehydrogenase / Q000187:drug effects; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008560:Membrane Fluidity / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects; D011188:Potassium / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013053:Spectrophotometry; D013385:Succinate Dehydrogenase / Q000187:drug effects
D003580:Cytochromes; D002104:Cadmium; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase; D013385:Succinate Dehydrogenase; D005969:Glutamate Dehydrogenase; D011188:Potassium