title: Protease-mediated degradation of lignin peroxidase in liquid cultures of Phanerochaete chrysosporium.
abstract: The decline of lignin peroxidase (LiP) activity observed after day 6 in cultures of Phanerochaete chrysosporium was found to be correlated with the appearance of idiophasic extracellular protease activity. Daily addition of glucose started on day 6 resulted in low protease levels and in turn in stable LiP levels. Addition of cycloheximide to day 6 cultures resulted in virtually no change of LiP activity and extracellular protein and negligible levels of protease activity, indicating that this protease is synthesized de novo. LiP activity was found to be stable upon removal of the fungal pellets on day 6 and incubation of the extracellular fluid alone. An almost complete disappearance of LiP activity and LiP proteins and high levels of protease activity were observed upon incubation of 6-day extracellular fluid in the presence of fungal pellets. Moreover, incubation of crude or purified LiP isoenzymes with protease-rich extracellular fluid of day 11 or 11-day cell extracts resulted in a marked loss of activity. In contrast, incubation of crude LiP with boiled and clarified extracellular fluid of day 11 cultures resulted in virtually no loss of activity. These results indicate that protease-mediated degradation of LiP proteins is a major cause for the decay of LiP activity during late secondary metabolism in cultures of P. chrysosporium.
authors: Department of Chemical Engineering, Michigan State University, East Lansing 48824.
authors: C G
authors: CG
authors: Dosoretz
authors: S B
authors: SB
authors: Dass
authors: C A
authors: CA
authors: Reddy
authors: H E
authors: HE
authors: Grethlein
mesh_terms: D001487:Basidiomycota / Q000187:drug effects / Q000378:metabolism*; D010450:Endopeptidases / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000378:metabolism; D007700:Kinetics; D008031:Lignin / Q000378:metabolism; D010544:Peroxidases / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D008031:Lignin; D010544:Peroxidases; C042858:lignin peroxidase; D010450:Endopeptidases; D005947:Glucose