[Morphofunctional changes in the lymph nodes and microcirculatory bed of small-intestinal mesentery of dogs in septic peritonitis after endolymphatic administration of ampicillin].
With the aim to study effectiveness+ of endolymphatic (EL) administration of ampicillin (AC), using the model of an acute diffuse septic peritonitis in dogs, the morphological and morphometrical investigation has been performed concerning the state of the lymph nodes (LN), which are regional as regards the pathological focus (pelvic) and remote (tracheobronchial, mesenteric) and hemomicrocirculatory bed (HMCB) of the small intestine mesentery. All LN groups studied are involved in the pathological process, that produces certain increasing disturbances in the structure and cell composition in LN. In 6 h the changes are especially manifested in the pelvic LN, and in 18 h--in the animals without application of AC, or at its intramuscular injection LN lose their typical structure. Their dimensions and number of lymphoid nodules++ and medullary cords decrease, a sharp impoverishment of lymphocytes in LN is observed. By this time critical disturbances in the HMCB structure develop; they are characterized as presence of great amount of avascular areas in the mesentery, extended capillary loops, plasmatic saturation of interstitium. When AC is injected endolymphatically, simultaneously with peritonitis modelling T- and B-dependent zones in LN are preserved, a high volumetric part of lymphocytes is kept in all groups of LN, structure and function of HMCB are normalized. The pronounced delay in development and decreasing manifestation of infective-toxic disorder in LN and HMCB depend on effective concentrations of the antibiotic, produces in the lymphatic system.
Shcherbakova
E G
EG
Kruglova
I S
IS
Larin
B A
BA
Zhuravleva
T P
TP
Rastunova
G A
GA
Shishlo
V K
VK
D000208:Acute Disease; D000667:Ampicillin / Q000008:administration & dosage*; D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D007272:Injections, Intralymphatic; D007421:Intestine, Small / Q000098:blood supply / Q000187:drug effects / Q000473:pathology*; D008198:Lymph Nodes / Q000187:drug effects / Q000473:pathology* / Q000503:physiopathology; D008297:Male; D008643:Mesentery / Q000098:blood supply / Q000187:drug effects / Q000473:pathology*; D008833:Microcirculation / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D010538:Peritonitis / Q000188:drug therapy / Q000473:pathology* / Q000503:physiopathology
D000667:Ampicillin
Morfofunktsional'nye izmeneniia limfaticheskikh uzlov i gemomikro- tsirkuliarnogo rusla bryzheĭki tonkoĭ kishki sobak na fone septicheskogo peritonita pri éndolimfaticheskom vvedenii ampitsillina.