The ADE2 gene from Saccharomyces cerevisiae: sequence and new vectors.
We have determined the sequence of a DNA fragment encoding the ADE2 gene from Saccharomyces cerevisiae. A DNA fragment of 2241 bp capable of complementing ade2 mutations was modified so it is available as a single BglII fragment for use in yeast vectors or for gene disruptions. The minimal fragment codes for a putative protein which is highly similar to the protein encoded by the ADE6 gene from Schizosaccharomyces pombe and to the proteins encoded by the purEK operon of Escherichia coli.
Stotz
A
A
Department of Microbiology, Biozentrum, Basel, Switzerland.
Linder
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P
D000595:Amino Acid Sequence; D001483:Base Sequence; D002262:Carboxy-Lyases / Q000378:metabolism*; D002872:Chromosome Deletion; D004926:Escherichia coli / Q000235:genetics; D005800:Genes, Fungal; D008040:Genetic Linkage; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D002262:Carboxy-Lyases; C022378:phosphoribosylaminoimidazole carboxylase