Replication timing of DNA sequences associated with human centromeres and telomeres.
The timing of replication of centromere-associated human alpha satellite DNA from chromosomes X, 17, and 7 as well as of human telomeric sequences was determined by using density-labeling methods and fluorescence-activated cell sorting. Alpha satellite sequences replicated late in S phase; however, the alpha satellite sequences of the three chromosomes studied replicated at slightly different times. Human telomeres were found to replicate throughout most of S phase. These results are consistent with a model in which multiple initiations of replication occur at a characteristic time within the alpha satellite repeats of a particular chromosome, while the replication timing of telomeric sequences is determined by either telomeric origins that can initiate at different times during S phase or by replication origins within the flanking chromosomal DNA sequences.
Ten Hagen
K G
KG
Department of Genetics, Stanford University School of Medicine, California 94305-5120.
Gilbert
D M
DM
Willard
H F
HF
Cohen
S N
SN
D000818:Animals; D015139:Blotting, Southern; D002453:Cell Cycle; D002460:Cell Line; D002886:Chromosomes, Human, Pair 17; D002897:Chromosomes, Human, Pair 7; D004261:DNA Replication; D004276:DNA, Satellite / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology; D007700:Kinetics; D008297:Male; D051379:Mice; D012091:Repetitive Sequences, Nucleic Acid; D013997:Time Factors; D014960:X Chromosome
D004276:DNA, Satellite