Estimation of allowable errors for tilt parameter determination in protein electron crystallography.
A three-dimensional reconstruction of a thin protein crystal requires an accurate assignment of amplitudes and phases in the three-dimensional reciprocal space. This assignment depends upon the tilt parameters which are experimentally determined. A theoretical estimate is made of the tolerable error in the determination of tilt parameters as a function of resolution and crystal thickness.
Prasad
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Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, TX 77030.
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Jeng
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Chiu
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D003460:Crystallization; D003720:Densitometry; D016018:Least-Squares Analysis; D011506:Proteins / Q000737:chemistry* / Q000648:ultrastructure; D014961:X-Ray Diffraction
D011506:Proteins