Affinity purification of a 70K protein, a membrane protein relevant to sexual cell fusion in Dictyostelium discoideum.
Sexual cell fusion occurs between HM1 and NC4, heterothallic strains in Dictyostelium discoideum. A membrane component of HM1 cells with a molecular mass of 70 kDa (70K protein) has been shown to be implicated in cell fusion (Urushihara et al. (1988) Cell Differ. Dev. 25, 81-88). In the present study, 70K protein was partially purified using affinity Sepharose on which membrane proteins of NC4 cells were immobilized. Through this process, involvement of Ca2+ in the interaction of 70K protein with its receptor was suggested. Lectin staining of partially purified 70K protein indicated it to be a glycoprotein containing D-mannose and/or D-glucose residues.
Ishikawa
T
T
University of Tsukuba, Institute of Biological Sciences, Ibaraki, Japan.
Urushihara
H
H
Habata
Y
Y
Yanagisawa
K
K
D002118:Calcium / Q000502:physiology*; D002459:Cell Fusion; D002846:Chromatography, Affinity; D004023:Dictyostelium / Q000502:physiology*; D005656:Fungal Proteins / Q000302:isolation & purification*; D008562:Membrane Glycoproteins / Q000302:isolation & purification* / Q000502:physiology; D012098:Reproduction
D005656:Fungal Proteins; D008562:Membrane Glycoproteins; D002118:Calcium