Characterization of skin-infiltrating T lymphocytes during acute graft-versus-host disease.
A panel of 34 clones was established from a cell line derived from the skin biopsy of a patient (genotype: A1, A2, B7, B8, DR3, DR6) undergoing acute graft-versus-host disease after semiallogeneic bone marrow transplantation with his mother's bone marrow (genotype: A1, A1, B7, B8, DR3, DR6). The T-cell line obtained presented the following phenotype: CD3+, CD4+, CD8-, CD16-, WT31+, T-cell receptor delta 1-, 4B4+, 2H4-, CD25+, DR+. This CD4+ T-cell line was poorly cytotoxic against the target cells tested, including the mother's phytohemagglutinin blasts as a negative control (autologous T cells), the father's phytohemagglutinin blasts bearing the mismatch haplotype, K562, U937, SVK14 (a keratinocyte cell line), and a panel of B-lymphoblastoid cell lines bearing HLA-A2, the known mismatch antigen. All but 1 of the 34 clones obtained were of CD4+ phenotype, and none was CD16+. Only the sole CD8+ clone showed significant cytotoxicity against the father's phytohemagglutinin blast; however, this cytotoxic activity was associated with the highest score for nonspecific killing against both K562 and U937. This work demonstrates the feasibility of obtaining a large panel of clones from a graft-versus-host disease target organ to constitute the basic cellular material for in vitro study of the graft-versus-host process.
Vié
H P
HP
INSERM U211, Faculté de Médecine, Nantes, France.
Millpied
N
N
Devilder
M C
MC
Mahe
B
B
Hallet
M M
MM
Moreau
J F
JF
Soulillou
J P
JP
D000208:Acute Disease; D016026:Bone Marrow Transplantation; D002478:Cells, Cultured; D002648:Child; D002999:Clone Cells / Q000473:pathology; D003601:Cytotoxicity Tests, Immunologic; D006086:Graft vs Host Disease / Q000473:pathology*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000473:pathology / Q000601:surgery; D008297:Male; D012867:Skin / Q000473:pathology*; D016176:T-Lymphocyte Subsets; D013601:T-Lymphocytes / Q000473:pathology*