title: Characterization of leukocyte chemotactic activity of bacteriocin from Streptococcus mutans Rm-10.
abstract: Bacteriocins have several biological activities in addition to their antibacterial effect. We investigated the chemotactic properties and mode of action of purified streptococcal bacteriocin. Bacteriocin purified from a culture supernatant of Streptococcus mutans (S. mutans) Rm-10 induced chemotaxis of human polymorphonuclear leukocytes and monocytes. Following purification, leukocyte migration appeared in one bacteriocin fraction, and this migration was dependent on the concentration gradient in dilution ranges from 1/960 to 1/15. Chemotactic activity of the bacteriocin was heat labile and trypsin sensitive. Moreover, preincubation of bacteriocin with varying dilutions of its antiserum prepared in rabbits resulted in a significant loss of the chemotactic activity.
authors: Department of Pharmacology, Osaka Dental University, Japan.
authors: Y
authors: Y
authors: Tsukamoto
authors: S
authors: S
authors: Fukutani
authors: Y
authors: Y
authors: Takayama
authors: H
authors: H
authors: Fukushima
authors: H
authors: H
authors: Sagawa
authors: M
authors: M
authors: Mori
mesh_terms: D001430:Bacteriocins / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D006801:Humans; D007106:Immune Sera; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects; D013295:Streptococcus mutans / Q000032:analysis*
chemical_list: D001430:Bacteriocins; D007106:Immune Sera