[Phospholipase A2 activating factor in sera of patients with psoriasis vulgaris].
Phospholipase A2 (PL-A2) has been known to be activated in either affected or un-affected skin of patients with psoriasis vulgaris. The present study was designated to investigate whether or not there is a factor activating PL-A2 in their sera, using cultured murine epidermal cells (NCTC-2544). The cultured cells prelabeled with 3H-arachidonic acid (3H-AA). After adding a serum, the release of 3H-AA into medium from the cells was measured. The sera from 20 patients with psoriasis vulgaris showed significantly increased releases of 3H-AA as compared with those by sera from 10 healthy individuals, indicating that there may be a PL-A2 activating factor in the sera of psoriasis vulgaris. The PL-A2 activating factor in the patient sera was not inactivated with added 2 different PL-A2 inhibitors. The sera were not able to produce 3H-AA from 3H-AA labeled phospholipids. Therefore, PL-A2 activating factor does not seem to be PL-A2 itself existing in the sera. Through heat stability, dialysis, and fractionation studies, the PL-A2 activating factor in sera was found to be a protein, which had a molecular weight of more than 50 kd and was stable at 56 degrees C.
Kobayashi
S
S
Department of Dermatology, Niigata University School of Medicine.
D000293:Adolescent; D000328:Adult; D000368:Aged; D001095:Arachidonic Acids / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D011565:Psoriasis / Q000097:blood* / Q000209:etiology; D012867:Skin / Q000378:metabolism
D001095:Arachidonic Acids; D011506:Proteins; C055324:phospholipase A2-activating protein; D010741:Phospholipases A; D054467:Phospholipases A2