Capillary gas-liquid chromatography separation of phenethylamines in amphetamine-positive urine samples.
Good gas chromatography (GC) separation of molecules is essential for clean gas chromatography/mass spectrometry (GC/MS) confirmation of compounds. The trifluoro derivatives of ephedrine (E) and methamphetamine (MA) coelute on dimethyl silicone capillary columns, such as DB-1, which are most commonly used by chromatographers. Methods are described to separate E and MA to aid GC/MS confirmations of methamphetamine, ephedrine, or both E and MA together, whichever may be present in Enzyme Immunoassay (EIA)-analyzed amphetamine-positive urine samples. The use of the heptafluoro derivatives of E and MA on a DB-1 column, or the trifluoro derivatives of E and MA on a DB-17 column, is suggested for good gas chromatographic separation.
DePace
A
A
New York State Psychiatric Institute, New York.
Verebey
K
K
elSohly
M
M
D000661:Amphetamine / Q000652:urine*; D002849:Chromatography, Gas / Q000379:methods*; D004809:Ephedrine / Q000737:chemistry; D006801:Humans; D010627:Phenethylamines / Q000737:chemistry*; D011237:Predictive Value of Tests
D010627:Phenethylamines; D000661:Amphetamine; D004809:Ephedrine