Iron detoxifying activity of ferritin. Effects of H and L human apoferritins on lipid peroxidation in vitro.
Three recombinant human apoferritin variants were added to ferrous iron and the amount of lipid peroxidation produced by hydrogen peroxide was studied. The H-apoferritin had the strongest inhibitory effect on lipid peroxidation, probably due to its ferroxidase activity. The L-apoferritin inhibited lipid peroxidation slowly and only at neutral pH. The H-mutant 91, deleted of the last 22 C-terminal amino acids, and which is not able to form an iron core, had minimal effects on iron lipid peroxidation. It was concluded that both ferro-oxidase and iron mineralization activities are necessary for ferritin iron detoxifying action.
Cozzi
A
A
Department of Biomedical Science and Technology, University of Milano, San Raffaele Institute, Italy.
Santambrogio
P
P
Levi
S
S
Arosio
P
P
D001052:Apoferritins / Q000378:metabolism; D004252:DNA Mutational Analysis; D005293:Ferritins / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007501:Iron / Q000378:metabolism; D007700:Kinetics; D008054:Lipid Peroxides / Q000378:metabolism*; D011994:Recombinant Proteins
D008054:Lipid Peroxides; D011994:Recombinant Proteins; D005293:Ferritins; D001052:Apoferritins; D007501:Iron