Effect of vitamin A deficiency on the in vitro transfer of mannose and N-acetylglucosamine in rat liver nuclei.
Liver nuclei, prepared from normal and vitamin A-deficient rats, were incubated in the presence of GDP-(14C)mannose or UDP-N-acetyl(14C)glucosamine and the labelled glycoproteins analysed by SDS PAGE. Fluorographic analysis has shown that (14C) mannose labelling is enhanced by vitamin A deficiency whereas N-acetyl(14C)glucosamine transfer remains approximately at the same level regardless of the vitamin A status; we did not notice any modification when the proteins were monitored by Coomassie blue or by silver nitrate.
Galland
S
S
Laboratoire de Biochimie des Membranes, Université Cl. Bernard Lyon I, Villeurbanne, France.
Fayet
Y
Y
Degiuli
A
A
Got
R
R
Azzar
G
G
Letoublon
R
R
Frot-Coutaz
J
J
Nicol
M
M
Azais-Braesco
V
V
Pascal
G
G
D000117:Acetylglucosamine / Q000378:metabolism*; D000818:Animals; D001835:Body Weight; D004032:Diet; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis; D006031:Glycosylation; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014801:Vitamin A / Q000008:administration & dosage; D014802:Vitamin A Deficiency / Q000378:metabolism*
D006023:Glycoproteins; D014801:Vitamin A; D008358:Mannose; D000117:Acetylglucosamine