Comparison of two preparatory techniques for urine cytology.
Two methods of preparation of urine for cytology were compared retrospectively. In method 1 cells in the urine were fixed after the preparation of the smear; in method 2 the cells were fixed before smear preparation. Urine cytology reports were correlated with subsequent histological analysis. The specificities of urine cytology using both methods were high (99%). The sensitivity using method 1 was 87%; using method 2 it was 65%. This difference was significant. The cell preparation technique therefore significantly changes the sensitivity of urine cytology. Cellular fixation after smear preparation is preferable to smear preparation after fixation.
Dhundee
J
J
Department of Cytology, Southmead Hospital, Bristol.
Rigby
H S
HS
D003584:Cytological Techniques; D005404:Fixatives; D006801:Humans; D011309:Preservation, Biological / Q000379:methods; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D014556:Urine / Q000166:cytology*; D014571:Urologic Neoplasms / Q000652:urine*
D005404:Fixatives