title: A rapid procedure for eliminating chromatofocusing buffer and concentrating minor active subforms of mitochondrial malate dehydrogenase.
abstract: Mitochondrial malate dehydrogenase from several sources contains different molecular forms whose origin is still under discussion. Separation of these subforms has been achieved by chromatofocusing. A simple and rapid method, based on 5' AMP Sepharose chromatography, has been developed to concentrate mitochondrial malate dehydrogenase subforms and simultaneously remove chromatofocusing buffer.
authors: Department de Bioqúimica i Fisiologia, Facultat de Química, Universitat de Barcelona, Spain.
authors: J L
authors: JL
authors: Gelpí
authors: V
authors: V
authors: Gracia
authors: S
authors: S
authors: Imperial
authors: A
authors: A
authors: Mazo
authors: A
authors: A
authors: Cortés
mesh_terms: D000818:Animals; D002021:Buffers; D002645:Chickens; D002846:Chromatography, Affinity / Q000379:methods*; D007525:Isoelectric Focusing; D008291:Malate Dehydrogenase / Q000302:isolation & purification* / Q000378:metabolism; D008930:Mitochondria, Liver / Q000201:enzymology
chemical_list: D002021:Buffers; D008291:Malate Dehydrogenase