Application of micellar mobile phases for the assay of drugs in biological fluids.
Although micellar chromatography has been used for the determination of drugs in biological fluids since 1985, relatively few researchers have applied the technique to therapeutic monitoring. The reasons for this are rather unclear. It may be that most of the present extraction/reconstitution techniques are well established or that the method development procedure is unfamiliar. Significantly lower detection limits can be obtained with micellar mobile phases and column switching than with micellar mobile phases alone. Only two groups have used micellar mobile phases in conjunction with column switching for the determination of drugs in biological fluids. Since column switching with micellar mobile phases is a relatively new and untried technique, it will take some time before the full range of its applicability and limitations are known.
Koenigbauer
M J
MJ
ICI Pharmaceutical Groups, ICI Americas Inc., Wilmington, DE 19897.
D001826:Body Fluids / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D008823:Micelles; D004364:Pharmaceutical Preparations / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D008823:Micelles; D004364:Pharmaceutical Preparations