The 12 kDa protein of potato virus M displays properties of a nucleic acid-binding regulatory protein.
The 3' terminal 1.4 kb segment of potato virus M (PVM) genomic RNA was cloned and sequenced. This part of the viral genome encodes the capsid protein CP as well as a 12 kDa protein of as yet unknown function. Both proteins were expressed in bacteria and their nucleic acid-binding properties studied. The 12 kDa protein (pr12), but not the capsid protein bound single- and double-stranded nucleic acids. This property of pr12 in conjunction with a zinc finger motif located adjacent to a basic region of the 12 kDa protein suggests that it may act as a regulatory factor during virus replication.
Gramstat
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Max-Planck-Institut für Züchtungsforschung, Köln, FRG.
Courtpozanis
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A
Rohde
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W
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010942:Plant Viruses / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D011487:Protein Conformation; D012367:RNA, Viral / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism; D014779:Virus Replication; D016335:Zinc Fingers
D004279:DNA, Viral; D012367:RNA, Viral; D014764:Viral Proteins