Human platelet glycoprotein IX. Characterization of cDNA and localization of the gene to chromosome 3.
Overlapping cDNAs encoding human platelet glycoprotein (Gp)IX were cloned from a human erythroleukemia cell lambda gt11 library. The possibly 'full-length' cDNA of 896 base pairs (bp) includes an open reading frame (528 bp), both 5' (222 bp) and 3' (146 bp) noncoding regions, and a poly(A) tail. Translation predicts a signal peptide of 16 amino acids and a mature protein of 160 amino acids that includes a 24 amino acid leucine-rich glycoprotein (LRG) segment. Southern blot analysis suggests the presence of a single copy of the Gp IX gene, and hybridization of Gp IX cDNA to sorted human chromosomes localizes the Gp IX gene to chromosome 3.
Hickey
M J
MJ
Seattle Veterans Administration Medical Center, WA 98108.
Deaven
L L
LL
Roth
G J
GJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D004247:DNA / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010980:Platelet Membrane Glycoproteins / Q000235:genetics*; D011487:Protein Conformation; D015183:Restriction Mapping
D010980:Platelet Membrane Glycoproteins; D004247:DNA