In situ hybridization ascertains the presence of a translocation t(6;11) in an acute monocytic leukemia.
In situ hybridization was performed in a case of acute monoblastic leukemia (FAB type M5b) with a rearrangement of the long arm of chromosome 11. Cytogenetic analysis after R- and G-banding showed an apparent deletion of 11q with a breakpoint at 11q23, and a translocation t(6;11) was suspected in certain metaphases. In situ hybridization with a biotinylated cosmid probe hybridizing at 11q25 confirmed the translocation t(6;11)(q27;q23). Use of nonradioactive in situ hybridization techniques for more precise characterization of chromosomal rearrangements in malignant cells is emphasized.
Derré
J
J
Unité INSERM U 301, Institut de Génétique Moléculaire, Paris, France.
Cherif
D
D
Le Coniat
M
M
Julier
C
C
Berger
R
R
D001853:Bone Marrow / Q000648:ultrastructure; D002648:Child; D002871:Chromosome Banding; D002880:Chromosomes, Human, Pair 11; D002896:Chromosomes, Human, Pair 6; D003360:Cosmids; D004273:DNA, Neoplasm; D005260:Female; D006801:Humans; D007621:Karyotyping; D007948:Leukemia, Monocytic, Acute / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D014178:Translocation, Genetic
D004273:DNA, Neoplasm