[Cloning in Escherichia coli of a mitochondrial DNA fragment from Acremonium chrysogenum containing a region responsible for DNA replication].
A bireplicone plasmid pSU901,4.6 kb in length, was constructed on the basis of plasmid pUC19 and the pstIB fragment, 1.9 kb in length, from mitochondrial DNA of A. chrysogenum. Based on the hybrid plasmid pSU901 and kanamycin resistance determinant, an autonomically replicating vector for A. chrysogenum, a culture producing cephalosporin C, is being constructed.
Petiushenko
R M
RM
Ukhabotina
L S
LS
Krakhmaleva
I N
IN
Telesnina
G N
GN
Danilenko
V N
VN
Sazykin
Iu O
IuO
Navashin
S M
SM
D000164:Acremonium / Q000235:genetics* / Q000378:metabolism; D002511:Cephalosporins / Q000096:biosynthesis; D003001:Cloning, Molecular / Q000379:methods*; D004261:DNA Replication / Q000502:physiology*; D004271:DNA, Fungal / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D066298:In Vitro Techniques
D002511:Cephalosporins; D004271:DNA, Fungal; D004272:DNA, Mitochondrial; C025163:cephalosporin C
Klonirovanie v Escherichia coli fragmenta mitokhondrial'noĭ DNK Acremonium chrysogenum, soderzhashchego uchastok, otvetstvennyĭ za replikatsiiu.