A new one-step method for the isolation of human mononuclear cells.
This paper describes a radical new, one-step method for the routine isolation of the mononuclear cells from human blood in high yield and purity. In this procedure the blood sample is mixed with a Nycodenz separation medium prior to centrifugation. Erythrocytes and polymorphogranulocytes are pelleted during centrifugation leaving the mononuclear cells in the supernatant. The method can be used for a wide range of blood sample volumes and can be carried out with greater ease than the currently accepted methods.
Ford
T C
TC
Department of Biology, University of Essex, Colchester, U.K.
Rickwood
D
D
D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D006801:Humans; D007472:Iohexol; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D011134:Polysaccharides; D012965:Sodium Chloride
D011134:Polysaccharides; D007472:Iohexol; D012965:Sodium Chloride