Phenotypic differences and characteristics of pyelonephritogenic strains of Escherichia coli isolated from children and adults.
We examined the frequency of bacterial strains expressing cell surface hydrophobicity, P-fimbriae, mannose-resistant haemagglutination (MRHA), type I fimbriae, production of aerobactin, haemolysin synthesis (Hly), production of cytotoxic necrotising factor (CNF) and HeLa cell adherence in 126 strains of Escherichia coli isolated from children (n = 65) and women (n = 61) with acute non-obstructive pyelonephritis. Previous investigations have shown that pyelonephritogenic strains of E. coli more often express hydrophobic properties, P-fimbriae, MRHA, aerobactin-mediated iron uptake, Hly and CNF production than strains isolated from the faecal flora of healthy persons. The objective of the present study was to examine phenotypic differences between strains of E. coli obtained from children with their first episode of acute pyelonephritis and strains from women with non-obstructive acute pyelonephritis. Of the pyelonephritogenic strains of E. coli isolated from children, 98% expressed cell surface hydrophobic properties compared to 82% isolated from adults (P = 0.004). Strains from children and adults had the same ability to assimilate iron and equally often expressed P-fimbriae, MRHA and type I fimbriae. Strains from children with acute pyelonephritis more significantly expressed Hly (72%) and CNF (58%) than did pyelonephritogenic strains from adults (49 and 37% respectively, P = 0.013 and P = 0.028 respectively). The frequency of HeLa cell adherence was similar and low in both groups. The phenotype aerobactin+ Hly+ and Hly+CNF+ was found significantly more often in pyelonephritogenic strains from children than in strains from adults (P = 0.006 and P = 0.028 respectively).(ABSTRACT TRUNCATED AT 250 WORDS)
Jacobson
S H
SH
Department of Medicine, Karolinska Hospital, Stockholm, Sweden.
Katouli
M
M
Tullus
K
K
Brauner
A
A
D000328:Adult; D000368:Aged; D001431:Bacteriological Techniques; D002675:Child, Preschool; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D005260:Female; D006801:Humans; D007681:Kidney Papillary Necrosis / Q000382:microbiology*; D008875:Middle Aged; D010641:Phenotype; D013045:Species Specificity; D014774:Virulence