Cloning of cDNA and amino acid sequence of one of chicken cone visual pigments.
The chicken has four kinds of color visual pigments, in addition to rhodopsin. A chicken genomic DNA library was screened with cDNA of human red-sensitive pigment and a chicken genomic DNA fragment including rhodopsin exons 2, 3 and 4, and then a genomic DNA fragment encoding a visual pigment, possibly an iodopsin, was cloned. A cDNA library, constructed from chicken retina mRNA, was screened with the genomic DNA fragment and the cDNA of human red-sensitive pigment, and the cDNA encoding the pigment was cloned. The nucleotide sequence of this cDNA was similar to that of the human red-sensitive pigment, with identities of 78% for the nucleotide sequence and 84% for the amino acid sequence with human red-sensitive pigment.
Tokunaga
F
F
Department of Physics, Faculty of Science, Tohoku University, Miyagi, Japan.
Iwasa
T
T
Miyagishi
M
M
Kayada
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics*; D003001:Cloning, Molecular; D003360:Cosmids; D004247:DNA / Q000737:chemistry*; D005091:Exons; D015698:Genomic Library; D006801:Humans; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012168:Retinal Pigments / Q000235:genetics*; D012243:Rhodopsin / Q000235:genetics*; D017299:Rod Opsins; D012680:Sensitivity and Specificity; D012689:Sequence Homology, Nucleic Acid
D012168:Retinal Pigments; D017299:Rod Opsins; C034941:iodopsin; D004247:DNA; D012243:Rhodopsin