title: Hepoxilin A3 blocks the release of norepinephrine from rat hippocampal slices.
abstract: Hepoxilin A3 was previously shown to display neuromodulatory actions on rat hippocampal CA1 neurons, with hyperpolarization of the membrane potential, an increase in the amplitude and duration of the post-spike train after hyperpolarization and an increase in the inhibitory post synaptic potential. The present report describes new biochemical evidence of a presynaptic action of hepoxilin A3 in rat hippocampal slices prelabeled with [3H]-norepinephrine. Hepoxilin A3 on its own had a marginal effect on the release of label, but blocked release which was induced by 4-aminopyridine (4-AP). Prostaglandin E2 also behaved in a similar way. These results demonstrate that hepoxilins modulate neurotransmission in the mammalian CNS through both pre- and postsynaptic actions.
authors: Research Institute, Hospital for Sick Children, Toronto, Canada.
authors: C R
authors: CR
authors: Pace-Asciak
authors: L
authors: L
authors: Wong
authors: E J
authors: EJ
authors: Corey
mesh_terms: D015761:4-Aminopyridine / Q000494:pharmacology; D015126:8,11,14-Eicosatrienoic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D015232:Dinoprostone / Q000494:pharmacology; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009638:Norepinephrine / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013569:Synapses
chemical_list: C038243:8-hydroxy-11,12-epoxyeicosa-5,9,14-trienoic acid; D015761:4-Aminopyridine; D015126:8,11,14-Eicosatrienoic Acid; D015232:Dinoprostone; D009638:Norepinephrine