The effects of Peyer's patches inactivation in the rat on the development of antibody-forming cells to intestinal antigen.
The effects of Peyer's patch (PP) inactivation on local and systemic immune response in rats was investigated. A cauterization method has been developed to inactivate PP. Animals were primed intraperitoneally or intragastrically with trinitrophenyl-keyhole limpet haemocyanin (TNP-KLH) one day before cauterization, and were challenged intraintestinally two weeks later. The secondary immune response in the spleen, mesenteric lymph node (MLN) and intestinal villi was studied by immunohistochemistry. Histological observations on PP after inactivation showed that most T cells in the interfollicular area had disappeared, as had B cells in the follicle. T cells repopulated PP much slower than B cells. Complete recovery of PP occurred no earlier than 2 weeks after inactivation. The immunization experiments revealed higher numbers of anti-TNP antibody-forming cells (anti-TNP AFC) of IgM, IgG and IgA isotypes in the spleen of the PP-inactivated animals than in the controls. Very few anti-TNP AFC were found locally in the lamina propria of the intestinal villi or in the MLN in both groups. It is suggested that PP play a role in the regulation of systemic immune responses against intestinally-administered thymus-dependent antigen. Moreover, inactivation of PP could alter the antigen uptake by gut epithelium and the local antigen processing. As a result, an increased amount of antigen could reach the spleen eliciting a higher immune response.
Soesatyo
M
M
Department of Histology, Medical Faculty, Free University, Amsterdam, The Netherlands.
Biewenga
J
J
Jeurissen
S
S
Sminia
T
T
D000818:Animals; D000921:Antibody-Producing Cells / Q000473:pathology; D001323:Autoantibodies / Q000096:biosynthesis*; D001324:Autoantigens / Q000276:immunology*; D015551:Autoimmunity; D006241:Haptens; D006433:Hemocyanins / Q000276:immunology; D007108:Immune Tolerance; D007114:Immunization; D007422:Intestines / Q000276:immunology*; D008221:Lymphoid Tissue / Q000276:immunology / Q000473:pathology; D010581:Peyer's Patches / Q000276:immunology* / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains
D001323:Autoantibodies; D001324:Autoantigens; D006241:Haptens; C054401:trinitrophenyl keyhole limpet hemocyanin; D006433:Hemocyanins