Thiamine requirement of Eikenella corrodens.
The thiamine requirement for growth of Eikenella corrodens was investigated. Autoclaved thiamine at a concentration of 1.0 microgram/ml supported maximal growth whereas for the same growth, filter-sterilized thiamine was required at 50-100 micrograms/ml. Studies with two thiamine degradation products, 2-methyl-4-amino-5-hydroxymethylpyrimidine and 4-methyl-5-(B-hydroxyethyl) thiazole, indicated that selected strains grew poorly or not at all in the presence of either moiety alone. However, the two moieties at a combined concentration of 0.02 microgram/ml supported growth equivalent to that of 1.0 microgram/ml of autoclaved thiamine. The requirement for a high concentration of filter-sterilized thiamine may reflect a faulty thiamine uptake apparatus and the observed growth response may be due to the presence of the moieties in the commercial thiamine preparation.
Robertson
N L
NL
Department of Biological Sciences, Western Illinois University, Macomb 61455.
Keudell
K C
KC
D003470:Culture Media; D016124:Eikenella corrodens / Q000254:growth & development*; D011743:Pyrimidines / Q000378:metabolism; D013242:Sterilization; D013831:Thiamine / Q000378:metabolism*; D013833:Thiamine Monophosphate / Q000378:metabolism; D013835:Thiamine Pyrophosphate / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism
D003470:Culture Media; D011743:Pyrimidines; D013844:Thiazoles; C012572:2-(4-methyl-1,3-thiazol-5-yl)ethanol; D013833:Thiamine Monophosphate; C012068:toxopyrimidine; D013835:Thiamine Pyrophosphate; D013831:Thiamine