Effect of propionyl-L-carnitine on rat spinal cord ischaemia and post-ischaemic reperfusion injury.
In this study we have examined the effect of propionyl-L-carnitine (PC) on rat spinal cord ischaemia and post-ischaemic reperfusion injury by evaluating two lipid peroxidation indices, thiobarbituric acid reactive substances (TBARS) and diene conjugation, before and after the addition of an ADP-Fe+2 complex to spinal cord homogenates. Aerobic, ischaemic, and post ischaemic reperfusion rat spinal cord homogenates from PC treated and untreated animals did not show any statistically significant difference in their TBARS and conjugated diene content. The addition of the ADP-Fe+2 complex to these homogenates resulted in an increased production of both the lipid peroxidation indices, though the magnitude of such formation was related to the type of experimental intervention. The post-ischaemic reperfusion samples of untreated rats showed the highest TBARS and conjugated diene content, while ischaemic samples in either treated and untreated rats did not show any statistically significant difference with respect to the aerobic samples. The post-ischaemic reperfusion samples of treated rats showed a statistically significant decrease of TBARS and conjugated diene production in comparison to the untreated samples. In addition, PC was also able to partially inhibit TBARS and conjugated diene formation in linoleic acid micelles exposed to hemoglobin, though it did not protect albumin fragmentation from the irradiation of water with an X-ray source.
Arduini
A
A
Istituto di Scienze Biochimiche, Universitá degli Studi G. D'Annunzio Chieti, Italy.
Fernandez
E
E
Pallini
R
R
Mancinelli
G
G
Di Toppi
G S
GS
Belfiglio
M
M
Scurti
R
R
Federici
G
G
D000818:Animals; D002331:Carnitine / Q000031:analogs & derivatives* / Q000627:therapeutic use; D005609:Free Radicals; D066298:In Vitro Techniques; D007511:Ischemia / Q000188:drug therapy* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism; D013116:Spinal Cord / Q000098:blood supply*; D013858:Thiobarbiturates / Q000378:metabolism
D005609:Free Radicals; D013858:Thiobarbiturates; C003223:propionylcarnitine; C029684:thiobarbituric acid; D002331:Carnitine