Time course of changes in lung permeability and edema in the rat exposed to 100% oxygen.
Rats were exposed to 100% oxygen for up to 60 h to determine early changes in lung permeability leading to the development of pulmonary edema. The time course of development of increased solute flux was assessed by the clearance of 99mTc-labeled diethylenetriamine pentaacetate (99mTc-DTPA) from the lung and the accumulation of 125I-labeled albumin (125I-albumin) in the lung. These end points were related to the development of pulmonary edema by the measurement of the wet-to-dry weight ratio of the lung and the weight of fluid in the pleural cavity. No significant changes occurred until 48 h of hyperoxia, when sharp increases in both indexes of lung permeability and wet-to-dry weight ratio occurred. By 60 h of exposure, pleural effusions had developed. The volume of this effusion was significantly correlated to both 99mTc-DTPA clearance and 125I-albumin flux.
Royston
B D
BD
Division of Anaesthesia, Clinical Research Centre, Harrow, Middlesex, United Kingdom.
Webster
N R
NR
Nunn
J F
JF
D000418:Albumins / Q000378:metabolism; D000818:Animals; D001835:Body Weight / Q000502:physiology; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D008168:Lung / Q000187:drug effects*; D008297:Male; D009929:Organ Size / Q000502:physiology; D010100:Oxygen / Q000494:pharmacology* / Q000633:toxicity; D010539:Permeability; D010996:Pleural Effusion / Q000503:physiopathology; D011654:Pulmonary Edema / Q000139:chemically induced / Q000503:physiopathology*; D051381:Rats; D011919:Rats, Inbred Strains; D016284:Technetium Tc 99m Pentetate
D000418:Albumins; D010100:Oxygen; D016284:Technetium Tc 99m Pentetate