Use of fetal mouse salivary glands in culture to detect embryotoxicity: evaluation of eight additional chemicals.
Several developmental processes interact to convert an epithelial bud into a gland with many lobes. For each chemical tested, 20 glands were placed into a control and each of 3 concentrations of the chemical. From dose-response curves, the concentration that reduced gland growth by 50% was determined and divided into the LD50 for mice. These ratios were used to compare the toxicity of the chemicals. The ratios of cyclamate, diphenhydramine, allopurinol, nitrofen and urethane would indicate that embryotoxicity would not be expected without maternal toxicity. Promethazine, diethyldithiocarbamate, and 5-fluorouracil would be expected to show embryotoxicity without maternal toxicity.
Lyng
R D
RD
Department of Biological Sciences, Indiana University-Purdue University, Ft. Wayne 46805.
D000818:Animals; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian; D007928:Lethal Dose 50; D051379:Mice; D012469:Salivary Glands / Q000187:drug effects* / Q000196:embryology; D013723:Teratogens / Q000145:classification / Q000633:toxicity*
D013723:Teratogens