Methionine dependence of rat hepatocarcinoma cells in culture: relationship with tumourigenicity and oncogene expression.
The culture in methionine-deprived medium of a rat hepatoma cell line (LF) significantly decreases: (1) the growth in vitro of these cells; (2) their tumourigenicity; and (3) the expression of a panel of oncogenes. When the LF cells are replaced in a medium containing methionine, they maintain a diminished in vitro growth and tumourigenic capacity, despite an overexpression of the same oncogenes. These data point out the determinant role of a nutritional factor, methionine, in tumour growth.
Painchault
V
V
Institut de Recherches Scientifiques sur le Cancer, CNRS UPR 278, Villejuif France.
Estrade
S
S
Frayssinet
C
C
Lafarge-Frayssinet
C
C
Cassingena
R
R
D000818:Animals; D002460:Cell Line; D015870:Gene Expression; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008715:Methionine / Q000172:deficiency* / Q000494:pharmacology; D009857:Oncogenes / Q000187:drug effects*; D051381:Rats
D008715:Methionine