Location of the suprachiasmatic nucleus grafts in rats which restored circadian rhythmicity after transplantation.
In Wistar male rats whose circadian wheel running activity rhythms were disrupted by bilateral suprachiasmatic nuclei (SCN) lesions, transplantation of neonatal rat SCN into the 3rd ventricle was performed. Out of 49 rats from which adequate wheel running activity records were obtained, 15 rats showed restoration of circadian rhythm starting 2 to 13 weeks (average 1 month) after transplantation. The existence of active SCN neurons in the graft was shown by immunocytochemical reactivity to vasopressin- and VIP-like substances. In all rats, effective grafts were found in the diencephalon, mostly on the wall of the 3rd ventricle.
Saitoh
Y
Y
Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Nihonmatsu
I
I
Kawamura
H
H
D000818:Animals; D000831:Animals, Newborn / Q000502:physiology; D016380:Brain Tissue Transplantation; D002552:Cerebral Ventricles / Q000502:physiology; D002940:Circadian Rhythm / Q000502:physiology*; D007031:Hypothalamus / Q000502:physiology; D008297:Male; D009043:Motor Activity / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D013493:Suprachiasmatic Nucleus / Q000502:physiology*