title: Exon trapping: a genetic screen to identify candidate transcribed sequences in cloned mammalian genomic DNA.
abstract: Identification and recovery of transcribed sequences from cloned mammalian genomic DNA remains an important problem in isolating genes on the basis of their chromosomal location. We have developed a strategy that facilitates the recovery of exons from random pieces of cloned genomic DNA. The basis of this "exon trapping" strategy is that, during a retroviral life cycle, genomic sequences of nonviral origin are correctly spliced and may be recovered as a cDNA copy of the introduced segment. By using this genetic assay for cis-acting sequences required for RNA splicing, we have screened approximately 20 kilobase pairs of cloned genomic DNA and have recovered all four predicted exons.
authors: Department of Pediatrics, University of California, San Francisco 94143-0554.
authors: G M
authors: GM
authors: Duyk
authors: S W
authors: SW
authors: Kim
authors: R M
authors: RM
authors: Myers
authors: D R
authors: DR
authors: Cox
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods*; D005091:Exons; D005822:Genetic Vectors; D015698:Genomic Library; D006680:HLA Antigens / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D012326:RNA Splicing
chemical_list: D006680:HLA Antigens