Cloning and nucleotide sequence of the Salmonella typhimurium pepM gene.
The pepM gene coding for a methionine-specific aminopeptidase was cloned from Salmonella typhimurium and its nucleotide sequence determined. The gene encoded a 264 amino acid protein that was homologous to a similar protein from Escherichia coli. The sequence of an overproducer mutant allele, pepM100, contained a single base change in the likely--35 region of the pepM promoter that increased its homology to the consensus promoter sequence. A region downstream from the pepM coding sequence contained extensive inverted repeats and was homologous to sequences found elsewhere in both Salmonella and other bacterial species.
Movva
N R
NR
Biogen S.A., Geneva, Switzerland.
Semon
D
D
Meyer
C
C
Kawashima
E
E
Wingfield
P
P
Miller
J L
JL
Miller
C G
CG
D000483:Alleles; D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D005800:Genes, Fungal; D063208:Methionyl Aminopeptidases; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic; D012486:Salmonella typhimurium / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D000626:Aminopeptidases; D063208:Methionyl Aminopeptidases