[The use of monoclonal antibodies against insulin for isolation of proteins inhibiting the cell growth].
Extracts of pig kidneys or germinated soya beans after preliminary steps were affinity chromatographied on Sepharose containing cyanogen bromide immobilized monoclonal antibodies to pig insulin. The material bound to the affinity column was separated by HPLC resulting in one homogeneous protein from each source. Both proteins have been shown to inhibit DNA synthesis in cultured embryonic human fibroblasts and VERO fibroblasts. The effect of pig kidney protein was potentiated by insulin. Soya and pig proteins were characterized by the following parameters: molecular weights of 8.5 and 10.3 kD, apparent constants of dissociation with rat liver plasma membranes of 4.7 x 10(-8) M and 9.8 X 10(-8) M, respectively. The soya proteins competed for the binding sites on plasma membranes with insulin whereas the pig protein did not. The N-terminal amino acid sequences of 20 residues were determined for both proteins. Comparison of these sequences with known protein sequences was performed. A 30-40% primary structure homology of the studied fragment of soya bean protein with the fragments of some oncogenic viruses proteins and transforming proteins was revealed.
Barbashov
S F
SF
Egorov
Ts A
TsA
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D000911:Antibodies, Monoclonal; D002455:Cell Division / Q000187:drug effects*; D002846:Chromatography, Affinity; D006801:Humans; D007330:Insulin Antibodies; D007668:Kidney / Q000737:chemistry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D014674:Plant Proteins, Dietary / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D011506:Proteins / Q000302:isolation & purification*; D051381:Rats; D030262:Soybean Proteins; D013552:Swine; D014709:Vero Cells
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D007330:Insulin Antibodies; D014674:Plant Proteins, Dietary; D011506:Proteins; D030262:Soybean Proteins
Ispol'zovanie monoklonal'nykh antitel k insulinu dlia vydeleniia belkov, ingibiruiushchikh kletochnyĭ rost.