Experimental and theoretical dynamics of isoelectric focusing: IV. Cathodic, anodic and symmetrical drifts of the pH gradient.
The production of anodic, cathodic and symmetrical drifts of a pH 3.5-10 gradient formed by isoelectric focusing in polyacrylamide gels is demonstrated experimentally by manipulation of the electrolyte concentrations. Experimental behavior is reproduced by computer simulation of a model mixture of 15 hypothetical carrier ampholytes whose pIs span the pH range 3-10. The mechanism which produces the drifts is elucidated and approaches to minimize such drifts are discussed. The data suggest why most experimentally observed drifts are cathodic.
Mosher
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RA
Center for Separation Science, University of Arizona, Tucson 85721.
Thormann
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D003198:Computer Simulation; D004566:Electrodes; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D010756:Phosphoric Acids; D015203:Reproducibility of Results; D012972:Sodium Hydroxide
D010756:Phosphoric Acids; D012972:Sodium Hydroxide; C030242:phosphoric acid