title: Soluble factors from rabbit spleen cells kill and lyse Treponema pallidum in vitro.
abstract: Antibody and complement immobilize (kill) Treponema pallidum in vitro. Recent evidence also documents immobilization by soluble factors released by activated macrophages and lymphocytes. Immune-mediated lysis of treponemes, however, has not been reported. The findings in this paper focus on apparent treponemal lysis by rabbit splenic cell preparations. Using cells from animals infected testicularly for 9 to 12 days, unfractionated splenic preparations, as well as adherent and nonadherent preparations, killed and lysed T. pallidum. Phagocytosis alone could not explain the detrimental effects of adherent cells. When cytochalasin B was used to block phagocytosis, decreases in treponemal numbers were still detected. In related studies, immune rabbit sera did not enhance treponemicidal activity of the adherent cells. To assess the specificity of these reactions, T. pallidum was incubated with two monocyte-like cell lines (human U937 and mouse P388D1). Neither cell line was detrimental, and treponemal numbers were not lowered. The soluble nature of the treponemicidal factors from adherent and nonadherent preparations was shown by physically separating these cells from the organisms and demonstrating treponemal killing and lysis. In summary, clearance of T. pallidum from infected tissues is probably at least partially attributed to macrophage phagocytosis. Our findings suggest another mechanism involving lytic factors secreted by activated adherent and nonadherent cells.
authors: Department of Medical Microbiology and Immunology, School of Medicine, University of Minnesota, Duluth 55812.
authors: T J
authors: TJ
authors: Fitzgerald
authors: B J
authors: BJ
authors: Elmquist
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D003571:Cytochalasin B / Q000494:pharmacology; D005374:Filtration; D007106:Immune Sera / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects; D011817:Rabbits; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D014210:Treponema pallidum / Q000276:immunology*
chemical_list: D007106:Immune Sera; D003571:Cytochalasin B