title: Interaction of hemoglobin with chloride and 2,3-bisphosphoglycerate. A comparative approach.
abstract: The equilibrium oxygen-binding properties of hemoglobins from reindeer (Rangifer tarandus tarandus), musk ox (Ovibos muschatos) and a bat (Rousettus aegyptiacus) have been investigated with special reference to the effect of heterotrophic ligands such as chloride and 2,3-bisphosphoglycerate [Gri(2,3)P2]. The results obtained with hemoglobins from reindeer and musk ox indicate that their low oxygen affinity and their insensitivity to Gri(2,3)P2 are not only an intrinsic property of the molecule, as proposed in the case of ruminant hemoglobins, but also the results of the interplay between chloride and Gri(2,3)P2 interactions. In other words, insensitivity of reindeer and musk ox hemoglobins to Gri(2,3)P2 is mainly due to a decreased affinity constant for this cofactor and to an increased affinity constant for chloride anions; this renders more effective the competition of chloride for th anion-binding site. On the other hand bat hemoglobin behaves in a completely different way and could be regarded as a type case of low-affinity hemoglobin since its functional properties are modulated neither by chloride nor by Gri(2,3)P2. The results are discussed in the light of the amino acid residues which are known to be involved in the binding of organic phosphates.
authors: Department of Experimental Medicine and Biochemical Sciences, II University of Rome, Italy.
authors: B
authors: B
authors: Giardina
authors: O
authors: O
authors: Brix
authors: A
authors: A
authors: Colosimo
authors: R
authors: R
authors: Petruzzelli
authors: L
authors: L
authors: Cerroni
authors: S G
authors: SG
authors: Condo
mesh_terms: D000494:Allosteric Regulation; D000595:Amino Acid Sequence; D000818:Animals; D001188:Artiodactyla; D002685:Chiroptera; D002712:Chlorides / Q000378:metabolism*; D004163:Diphosphoglyceric Acids / Q000378:metabolism*; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D010100:Oxygen / Q000378:metabolism; D010108:Oxyhemoglobins / Q000378:metabolism*
chemical_list: D002712:Chlorides; D004163:Diphosphoglyceric Acids; D010108:Oxyhemoglobins; D010100:Oxygen