Immunoelectron microscopical localization of the catalytic subunit of cAMP-dependent protein kinases in brain microtubules and neurofilaments.
The catalytic subunit of cAMP-dependent protein kinases was localized in microtubules and neurofilaments by immunogold electron microscopy. In microtubules, the label was similarly distributed as an immunolabel for the microtubule associated protein MAP 2. The neurofilaments showed no reaction with the MAP 2-antiserum. Our results support the suggestion of an in vivo role of cAMP-dependent protein kinases in the regulation of microtubules. In addition, this is the first demonstration that cAMP-dependent protein kinase is associated with neurofilaments.
Trinczek
B
B
Zentrum Innere Medizin, Universität Göttingen, Germany.
Schwoch
G
G
D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000648:ultrastructure; D007150:Immunohistochemistry; D007382:Intermediate Filaments / Q000201:enzymology*; D008297:Male; D008870:Microtubules / Q000201:enzymology*; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D011494:Protein Kinases