Reversible ADP-ribosylation of the 78 kDa glucose-regulated protein.
Starvation of Mouse hepatoma cells for essential amino acids or glucose results in the mono-ADP-ribosylation of the 78 kDa glucose-regulated protein, GRP78. Here we show that the ADP-ribosylated and non-ADP-ribosylated forms of GRP78 are interconvertible during tryptophan starvation and refeeding. In addition, the ADP-ribosylation of GRP78 was shown to be reversible even during nutritional stress. The overexpressed pool of non-ADP-ribosylated GRP78 synthesized during tunicamycin treatment was available for ADP-ribosylation during subsequent amino acid starvation, especially in the absence of tunicamycin. The reversible ADP-ribosylation of GRP78 could be part of a metabolic control mechanism in operation during nutritional stress.
Leno
G H
GH
Molecular and Cellular Biology and Pathobiology Program, Medical University of South Carolina, Charleston 29425.
Ledford
B E
BE
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D000091342:Endoplasmic Reticulum Chaperone BiP; D018840:HSP70 Heat-Shock Proteins; D008114:Liver Neoplasms, Experimental; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000378:metabolism; D014415:Tunicamycin / Q000494:pharmacology
D000596:Amino Acids; D000091342:Endoplasmic Reticulum Chaperone BiP; D018840:HSP70 Heat-Shock Proteins; C000717248:Hspa5 protein, mouse; D008565:Membrane Proteins; D009363:Neoplasm Proteins; C018551:glucose-regulated proteins; D014415:Tunicamycin; D000246:Adenosine Diphosphate Ribose