Developments in techniques for radioiodination of peptide hormones and other proteins.
This article presents details on two currently used methods of labelling peptides with radioiodine. The solid phase enzymatic iodination with lactoperoxidase is very suitable for radioimmunoassays and receptor assays. The iodogen labelling technique has been successfully used in this laboratory for preparing radioiodinated radiopharmaceuticals. Other iodination methods are also briefly discussed.
Karonen
S L
SL
Department of Clinical Chemistry, University of Helsinki, Meilahti Hospital, Finland.
D004800:Enzymes, Immobilized; D006728:Hormones / Q000737:chemistry*; D006801:Humans; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D007553:Isotope Labeling / Q000379:methods*; D007784:Lactoperoxidase; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D014508:Urea / Q000031:analogs & derivatives
D004800:Enzymes, Immobilized; D006728:Hormones; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D010455:Peptides; D011506:Proteins; C012385:1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenylglycoluril; D014508:Urea; D007784:Lactoperoxidase