HPLC-photolysis-electrochemical detection in pharmaceutical analysis: application to the determination of spironolactone and hydrochlorothiazide in tablets.
To extend the applicability of electrochemical detection in pharmaceutical analysis, an on-line postcolumn photochemical reactor is used to produce electroactive photoderivatives of selected cardiovascular drugs. This system is applied to the analysis of dosage forms containing spironolactone in both single-component formulation and in combination with hydrochlorothiazide. The pH of the mobile phase and irradiation time within the reactor are optimized to produce maximum amperometric response. Spironolactone, hydrochlorothiazide, and 4-amino-6-chloro-1,3-benzenedisulfonamide, the synthetic precursor and hydrolysis product of hydrochlorothiazide, are separated by reversed-phase chromatography on a 5-microns cyano bonded phase column within nine minutes by using a methanol-phosphate buffer mobile phase. Minimum detectable levels are in the low ng range.
Bachman
W J
WJ
Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Georgia, Athens 30602.
Stewart
J T
JT
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D006852:Hydrochlorothiazide / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D000432:Methanol; D010710:Phosphates; D010782:Photolysis; D013148:Spironolactone / Q000032:analysis*; D013607:Tablets
D010710:Phosphates; D013607:Tablets; D006852:Hydrochlorothiazide; D013148:Spironolactone; D000432:Methanol