Sequence analysis of the potent mitogenic toxin of Pasteurella multocida.
Pasteurella multocida toxin is a potent mitogen for cultured Swiss 3T3 cells where it causes an accumulation of inositol phosphates and activation of protein kinase C. The gene sequence described here coded for a 146 kDa protein. The ORF was preceded by a ribosome binding site and followed by a stem loop. There was no evidence for a signal sequence. The gene had a low G + C base ratio which differs from the rest of the Pasteurella genome. There was no significant homology with other known proteins, although a motif found in certain bacterial toxins which are ADP-ribosyl transferases is present. A recombinant expressing only part of the PMT gene was not mitogenic.
Lax
A J
AJ
AFRC Institute for Animal Health, Compton, Newbury, Berkshire, UK.
Chanter
N
N
Pullinger
G D
GD
Higgins
T
T
Staddon
J M
JM
Rozengurt
E
E
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000235:genetics*; D001483:Base Sequence; D005798:Genes, Bacterial; D008934:Mitogens; D008969:Molecular Sequence Data; D010325:Pasteurella / Q000235:genetics*; D012995:Solubility
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D008934:Mitogens; C062258:Pasteurella multocida toxin