Target size of 5-HT3 receptors in N1E-115 neuroblastoma cells and rat brain.
Radiation inactivation was used to determine the molecular target size of the binding site for [3H]GR65630, a specific 5-HT3 receptor ligand, in two different neuronal tissues. Using a calibration curve of known molecular weight enzymes, the target sizes of [3H]GR65630 binding sites in N1E-115 neuroblastoma cells and rat brain were 98,600 +/- 11,300 and 49,100 +/- 8,500 Da, respectively. The results suggest 5-HT3 receptors may be present as dimers in N1E-115 neuroblastoma cells.
Lummis
S C
SC
Molecular Neurobiology Unit, MRC Centre, University of Cambridge Medical School, U.K.
Nielsen
M
M
Kilpatrick
G J
GJ
Martin
I L
IL
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D005445:Flunitrazepam; D007093:Imidazoles / Q000494:pharmacology; D007211:Indoles / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D008970:Molecular Weight; D009447:Neuroblastoma / Q000378:metabolism*; D011813:Quinuclidinyl Benzilate; D051381:Rats; D011985:Receptors, Serotonin / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000378:metabolism
D007093:Imidazoles; D007211:Indoles; D011985:Receptors, Serotonin; C059068:GR 65630; D005445:Flunitrazepam; D011813:Quinuclidinyl Benzilate