title: [Molecular-biological study of vaccinia virus genome. I. Cloning of vaccinia virus DNA fragments in bacterial vectors].
abstract: The HindIII DNA fragments of vaccinia virus strain L-IVP were cloned in pBR322 bacterial plasmid. A hybrid plasmids collection of pVHn series contains all fragments of virus genome except terminal HindIII-B and HindIII-G, and also a large HindIII-A. The latter was cloned in cosmid pHC79. The obtained collection of hybrid DNA molecules allows to carry out a wide range of molecular biological experiments on the vaccinia virus genome.
authors: O I
authors: OI
authors: Riazankina
authors: A V
authors: AV
authors: Totmenin
authors: S N
authors: SN
authors: Shchelkunov
authors: E G
authors: EG
authors: Malygin
mesh_terms: D015139:Blotting, Southern; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D015247:Deoxyribonuclease HindIII; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D005822:Genetic Vectors; D010957:Plasmids; D015183:Restriction Mapping; D014616:Vaccinia virus / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D015247:Deoxyribonuclease HindIII
vernacular_title: Molekuliarno-biologicheskoe izuchenie genoma virusa ospovaktsiny. I. Klonirovanie fragmentov DNK virusa ospovaktsiny v sostave bakterial'nykh vektorov.