Effect of diabetes in the BB Wistar rat on the peptidergic component of the enteric innervation.
Whole mount preparations of the small intestine from Wistar, normoglycemic 90-day diabetic BB, hyperglycemic 90-day diabetic BB and age-matched nondiabetic BB rats were immunostained to determine the extent of the peptidergic innervation. The neuropeptides examined were vasoactive intestinal peptide, neuropeptide Y, substance P. calcitonin gene-related peptide, galanin and somatostatin. The extent of the peptidergic innervation in the submucous and myenteric plexuses from the control Wistar, nondiabetic BB and normoglycemic diabetic BB rats was identical. In the submucous plexus of the hyperglycemic diabetic BB rats there was a marked reduction in the number of somatostatin-immunoreactive neurons and nerve fibres. Substance P and calcitonin gene-related peptide which are colocalized in a proportion of the somatostatin neurons were unaffected. In the myenteric plexus of the hyperglycemic diabetic BB rats the number and intensity of staining of the galanin-immunoreactive nerve fibers was markedly increased. Galanin has not been observed to colocalize with any of the other neuropeptides examined.
Buchan
A M
AM
Department of Physiology, University of British Columbia, Vancouver, Canada.
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D003922:Diabetes Mellitus, Type 1 / Q000503:physiopathology; D003929:Diabetic Neuropathies / Q000378:metabolism; D007421:Intestine, Small / Q000294:innervation*; D009197:Myenteric Plexus / Q000737:chemistry; D009474:Neurons / Q000737:chemistry; D009479:Neuropeptides / Q000378:metabolism*; D051381:Rats; D011913:Rats, Inbred BB; D013368:Submucous Plexus / Q000737:chemistry
D009479:Neuropeptides