Fine needle aspiration cytology and immunocytochemical characterization of the histiocytes in sinus histiocytosis with massive lymphadenopathy (Rosai-Dorfman syndrome).
A fine needle aspiration (FNA) biopsy of an enlarged lymph node in a three-year-old boy who had asymptomatic cervical and submandibular lymphadenopathy showed large histiocytes with abundant pale, eosinophilic cytoplasm containing well-preserved lymphocytes and occasional plasma cells and granulocytes. These features were consistent with a diagnosis of sinus histiocytosis with massive lymphadenopathy (SHML). Immunophenotypic study of the histiocytes, performed on the FNA smears and on paraffin-embedded sections, showed reactivity for S-100 protein and alpha-1-antichymotrypsin and negativity for lysozyme. These features, which are characteristic of SHML, demonstrate the reliability of FNA cytology in making the diagnosis of this disorder.
Pettinato
G
G
Department of Pathology, 2nd Medical School, University of Naples, Italy.
Manivel
J C
JC
d'Amore
E S
ES
Petrella
G
G
D015415:Biomarkers; D001707:Biopsy, Needle; D002675:Child, Preschool; D006644:Histiocytes / Q000473:pathology*; D015618:Histiocytosis, Sinus / Q000175:diagnosis / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000473:pathology*; D008297:Male; D009113:Muramidase / Q000032:analysis; D009418:S100 Proteins / Q000032:analysis*; D000514:alpha 1-Antichymotrypsin / Q000032:analysis*
D015415:Biomarkers; D009418:S100 Proteins; D000514:alpha 1-Antichymotrypsin; D009113:Muramidase