title: ADP-ribosylation of actin isoforms by Clostridium botulinum C2 toxin and Clostridium perfringens iota toxin.
abstract: The substrate specificities of the actin-ADP-ribosylating toxins, Clostridium botulinum C2 toxin and Clostridium perfringens iota toxin were studied by using five different preparations of actin isoforms: alpha-skeletal muscle actin, alpha-cardiac muscle actin, gizzard gamma-smooth muscle actin, spleen beta- and gamma-cytoplasmic actin, and aortic smooth muscle actin containing alpha- and gamma-smooth muscle actin isoforms. C. perfringens iota toxin ADP-ribosylated all actin isoforms tested, whereas C. botulinum C2 toxin did not modify alpha-skeletal muscle actin or alpha-cardiac muscle actin. Spleen beta/gamma-cytoplasmic actin and gizzard gamma-smooth muscle actin were substrates of C. botulinum C2 toxin. In the aortic smooth muscle actin preparation, gamma-smooth muscle actin but not alpha-smooth muscle actin was ADP-ribosylated by C. botulinum C2 toxin. The data indicate that, in contrast to C. perfringens iota toxin, C. botulinum C2 toxin ADP-ribosylates only beta/gamma-cytoplasmic and gamma-smooth muscle actin and suggest that the N-terminal region of actin isoforms define the substrate specificity for ADP-ribosylation by C. botulinum C2 toxin.
authors: Rudolf-Buchheim-Institut für Pharmakologie, Giessen, Federal Republic of Germany.
authors: S
authors: S
authors: Mauss
authors: C
authors: C
authors: Chaponnier
authors: I
authors: I
authors: Just
authors: K
authors: K
authors: Aktories
authors: G
authors: G
authors: Gabbiani
mesh_terms: D036002:ADP Ribose Transferases; D000199:Actins / Q000378:metabolism*; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000818:Animals; D001427:Bacterial Toxins / Q000378:metabolism*; D001905:Botulinum Toxins / Q000378:metabolism*; D003016:Clostridium perfringens; D015180:Electrophoresis, Gel, Two-Dimensional; D066298:In Vitro Techniques; D011817:Rabbits
chemical_list: D000199:Actins; D001427:Bacterial Toxins; C050737:iota toxin, Clostridium perfringens; D000246:Adenosine Diphosphate Ribose; D036002:ADP Ribose Transferases; D001905:Botulinum Toxins