Detection of a novel chloramphenicol resistance plasmid from "equine" Staphylococcus sciuri.
A small chloramphenicol resistance (Cm) plasmid of 4.65 kB could be detected in an "equine" Staphylococcus sciuri-culture. This plasmid, designated as pSC3, was identified by interspecific protoplast transformation. On the basis of restriction endonuclease analyses a detailed restriction map of pSC3 could be constructed. This allowed structural comparisons of pSC3 with Cm-plasmids of other staphylococcal species from infections of humans and animals and identification of pSC3 as a member of the pC 221-family of staphylococcal Cm-plasmids. The pSC3-plasmid encoded an inducible chloramphenicol acetyltransferase as confirmed by enzymatic assays. This enzyme could be demonstrated in cell-free lysates of Cm-induced pSC3-transformants.
Schwarz
S
S
Institut für Bakteriologie und Immunologie, Justus-Liebig-Universität Giessen, FRG.
Cardoso
M
M
Blobel
H
H
D000818:Animals; D002702:Chloramphenicol Resistance / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis; D006736:Horses; D011815:R Factors; D015183:Restriction Mapping; D013210:Staphylococcus / Q000187:drug effects / Q000235:genetics*
D004269:DNA, Bacterial