Effect of K252a, a protein kinase inhibitor, on the proliferation of vascular smooth muscle cells.
In the growth arrested cultures of bovine carotid smooth muscle, K252a (10 - 100 ng/ml), a protein kinase inhibitor with wide spectrum suppressed the cell proliferation induced by TPA and increase of serum. K252a was more potent in the antiproliferative activity than H7, a C-kinase-specific inhibitor, but less than staurosporine, another wide-spectrum protein kinase inhibitor. Since C-kinase plays an important role in the signal transduction leading to the cell proliferation and K252a inhibits C-kinase in vitro, the antiproliferative effect of K252a to carotid smooth muscle cells is likely to be exerted through c-kinase dependent pathway.
Ohmi
K
K
Department of Pharmacology, Faculty of Medicine, University of Tokyo, Japan.
Yamashita
S
S
Nonomura
Y
Y
D000818:Animals; D002227:Carbazoles / Q000494:pharmacology*; D002339:Carotid Arteries / Q000378:metabolism; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D026121:Indole Alkaloids; D009119:Muscle Contraction / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000098:blood supply / Q000187:drug effects / Q000502:physiology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors* / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects
D002227:Carbazoles; D026121:Indole Alkaloids; D004247:DNA; C049985:staurosporine aglycone; D011493:Protein Kinase C