Non-specific esterases in partly mineralized bovine enamel.
Activity for non-specific esterase was demonstrated in the matrix of developing bovine enamel with alpha-naphthyl acetate and 5-bromoindoxyl acetate as the esterase substrates. By use of high-performance liquid chromatography gel filtration, ion-exchange chromatography, and electrophoresis three esterases were shown to be present in the enamel matrix. The enzymes showed highest activity at pH 6.5-7.5. In sections a strong reaction was observed in the secretory ameloblasts. The esterases may be proteolytic enzymes that participate in the degradation of the matrix proteins.
Moe
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D
Institute of Biochemistry, Royal Dental College, Copenhagen, Denmark.
Kirkeby
S
S
D000566:Amelogenesis; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003743:Dental Enamel / Q000201:enzymology* / Q000648:ultrastructure; D003746:Dental Enamel Proteins / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004950:Esterases / Q000032:analysis*; D007211:Indoles; D009283:Naphthol AS D Esterase / Q000032:analysis; D013053:Spectrophotometry; D014083:Tooth Germ / Q000201:enzymology / Q000648:ultrastructure
D003746:Dental Enamel Proteins; D007211:Indoles; C056141:5-bromoindoxyl acetate; D004950:Esterases; D009283:Naphthol AS D Esterase