title: Hepatotoxic activity of Campylobacter jejuni.
abstract: Hepatotoxic factor(s) were isolated from whole-cell lysates of Campylobacter jejuni GIFU 8734 and purified by chromatography. A single intravenous injection of 10 micrograms of this factor reproducibly produced hepatitis in mice, as determined by histology and liver function tests. The hepatic lesions were very similar to those evoked by C. jejuni infection. Tissue-culture studies with mouse hepatocytes demonstrated that low concentrations of the factor caused release of hepatic enzymes into the medium without appreciable cytolysis. High concentrations of the factor induced cytolysis. These effects were neutralised by antiserum to the factor, but not by antisera to the lipopolysaccharide of C. jejuni or to the heat-labile enterotoxin of Escherichia coli. Among 20 clinical isolates of C. jejuni, only four evoked hepatitis in mice and produced the hepatotoxic factor.
authors: Department of Bacteriology, Nara Medical University, Japan.
authors: E
authors: E
authors: Kita
authors: D
authors: D
authors: Oku
authors: A
authors: A
authors: Hamuro
authors: F
authors: F
authors: Nishikawa
authors: M
authors: M
authors: Emoto
authors: Y
authors: Y
authors: Yagyu
authors: N
authors: N
authors: Katsui
authors: S
authors: S
authors: Kashiba
mesh_terms: D000418:Albumins / Q000096:biosynthesis; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D016123:Campylobacter jejuni / Q000472:pathogenicity*; D002470:Cell Survival; D002847:Chromatography, Agarose; D004305:Dose-Response Relationship, Drug; D004768:Enterotoxins / Q000494:pharmacology; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D008111:Liver Function Tests; D051379:Mice; D009500:Neutralization Tests
chemical_list: D000418:Albumins; D004768:Enterotoxins; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases