Effects of glycyl-L-glutamine and methylprednisolone on maintenance of acetylcholinesterase of transected rat sciatic nerves.
Under anesthesia with sodium pentobarbital, the sciatic nerves of rats were transected bilaterally, and a catheter was inserted into the central end of the left renal artery. After an initial flush, an Alzet pump was attached to the catheter, containing various concentrations of glycyl-L-glutamine (Gly-Gln), methylprednisolone sodium succinate (MePred), or both. Rats were sacrificed at intervals of 2, 4, or 6 days; the peripheral portions of the sciatic nerves were excised, homogenized, and centrifuged, and the supernates were assayed for acetylcholinesterase (AcChoEase; acetylcholine acetylhydrolase, EC 3.1.1.7) and protein. Significantly higher contents of AcChoEase over untreated transected controls were obtained (i) at 4 days posttransection in rats infused with 0.015 M Gly-Gln and (ii) at 6 days posttransection in rats infused with MePred at 3.0 mg.kg-1.hr-1 after an initial dose of 120 mg/kg with or without Gly-Gln.
Koelle
G B
GB
Department of Pharmacology, Medical School, University of Pennsylvania, Philadelphia 19014-6084.
Han
M S
MS
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D004151:Dipeptides / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D008775:Methylprednisolone / Q000494:pharmacology*; D051381:Rats; D012584:Sciatic Nerve / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*
D004151:Dipeptides; C039788:glycylglutamine; D000110:Acetylcholinesterase; D008775:Methylprednisolone