DNA polymerase activity of tomato fruit chromoplasts.
DNA polymerase activity was measured in chromoplasts of ripening tomato fruits. Plastids isolated from young leaves or mature red fruits showed similar DNA polymerase activities. The same enzyme species was present in either chloroplasts or chromoplasts as judged by pH and temperature profiles, sensitivities towards different inhibitors and relative molecular mass (Mr 88 kDa). The activities analyzed showed the typical behaviour of plastid-type polymerases. The results presented here suggest that chromoplast maintain their DNA synthesis potential in fruit tissue at chloroplast levels. Consequently, the sharp decrease of the plastid chromosome transcription observed at the onset of fruit ripening could not be due to limitations in the availability of template molecules. Other mechanisms must be involved in the inhibition of chromoplast RNA synthesis.
Serra
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Departamento de Ciencias Biológicas, Universidad Nacional de Rosario, Argentina.
Carrillo
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N
D002454:Cell Differentiation; D002736:Chloroplasts / Q000201:enzymology*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D019384:Nucleic Acid Synthesis Inhibitors; D010945:Plants, Edible / Q000201:enzymology*; D013696:Temperature
D019384:Nucleic Acid Synthesis Inhibitors; D004259:DNA-Directed DNA Polymerase