cis regulatory elements directing tuber-specific and sucrose-inducible expression of a chimeric class I patatin promoter/GUS-gene fusion.
The 5'-upstream region of the class I patatin gene B33 directs strong expression of the beta-glucuronidase (GUS) reporter gene in potato tubers and in leaves treated with sucrose. Cis-acting elements affecting specificity and level of expression were identified by deletion analysis in transgenic potato plants. A putative tuber-specific element is located downstream from position -195. Nuclear proteins present in leaf and tuber extracts bind specifically to a conserved AT rich motif within this region. A DNA fragment between -183 and -143, including the binding site is, however, not able to enhance the expression of a truncated 35S promoter from cauliflower mosaic virus. Independent positive elements contributing to a 100-fold increase relative to the basic tuber-specific element are located between -228 and -195; -736 and -509, -930 and -736 and -1512 and -951. Sucrose inducibility is controlled by sequences downstream of position -228, indicating that the tuber-specific and sucrose-inducible elements are in close proximity.
Liu
X J
XJ
Institut für Genbiologische Forschung Berlin GmbH, FRG.
Prat
S
S
Willmitzer
L
L
Frommer
W B
WB
D001665:Binding Sites; D002265:Carboxylic Ester Hydrolases; D002678:Chimera / Q000235:genetics; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000378:metabolism; D005786:Gene Expression Regulation; D006651:Histocytochemistry; D009154:Mutation; D010940:Plant Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D011198:Solanum tuberosum / Q000235:genetics* / Q000378:metabolism; D013395:Sucrose / Q000494:pharmacology*
D004268:DNA-Binding Proteins; D010940:Plant Proteins; C043222:patatin protein, Solanum tuberosum; D013395:Sucrose; D002265:Carboxylic Ester Hydrolases