title: Expression of epithelial antigens Exo-1 and EPM-1 in human epidermal keratinocyte maturation and benign and malignant neoplasia.
abstract: Exo-1, a polar neutral glycolipid, and EPM-1, a high molecular weight glycoprotein, are developmental antigens of human epithelial cells, initially described as components both on the cell surface and in secretions of gastrointestinal epithelial and respective tumors. In order to assess the biological significance of both antigens for epithelial cell differentiation and neoplastic transformation, their expression during human skin development and benign and malignant neoplasia was analyzed in fresh frozen tissue specimens of skin biopsies and of human epidermal keratinocytes growing in experimental model systems. Antigen expression was assessed immunohistochemically with specific monoclonal antibodies. During fetal development Exo-1 was temporarily expressed in intermediate cells but was absent in normal adult human skin. Exo-1 expression reemerged in neoplasias, both benign and malignant, but was restricted to spinous-like differentiated cells. Similarly, Exo-1 was not expressed in transplants of normal keratinocytes mimicking the normal epidermis but was clearly visible in differentiated areas of transplants of malignantly transformed keratinocytes. EPM-1 appeared first in basal epidermal cells in the second half of gestation and remained detectable in the stratum basale of adult skin. While squamous cell carcinomas continued to express EPM-1, it was not detectable in basal cell epitheliomas and in normal epidermis after invasion by neuroectodermal tumor cells. In experimental models, EPM-1 was present in the basal layers of normal human keratinocytes and of transformed keratinocytes with benign growth characteristics whenever a well stratified and keratinized epidermis-like epithelium had formed in transplants. In transformed keratinocytes with malignant growth behavior, EPM-1 was expressed irregularly, as in squamous cell carcinomas in situ. Thus, expression of Exo-1 is a marker for an early embryonic differentiation pathway of human keratinocytes and in adult tissue reveals abnormal differentiation associated with certain stages of hyperproliferation. EPM-1 expression is part of developmental programs and is influenced by microenvironmental interactions and alterations of tissue homeostasis.
authors: First Department of Internal Medicine, University of Mainz, Federal Republic of Germany.
authors: R
authors: R
authors: Klingel
authors: P
authors: P
authors: Boukamp
authors: R
authors: R
authors: Moll
authors: W
authors: W
authors: Tilgen
authors: N E
authors: NE
authors: Fusenig
authors: K H
authors: KH
authors: Meyer zum Büschenfelde
authors: W G
authors: WG
authors: Dippold
mesh_terms: D000941:Antigens / Q000096:biosynthesis*; D000954:Antigens, Surface / Q000096:biosynthesis; D002454:Cell Differentiation / Q000276:immunology; D002471:Cell Transformation, Neoplastic; D005314:Embryonic and Fetal Development / Q000276:immunology*; D015870:Gene Expression; D006197:Hair / Q000276:immunology; D006801:Humans; D006965:Hyperplasia / Q000276:immunology; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000276:immunology*; D011565:Psoriasis / Q000276:immunology; D012627:Sebaceous Glands / Q000276:immunology; D012867:Skin / Q000276:immunology; D012871:Skin Diseases / Q000276:immunology*; D012878:Skin Neoplasms / Q000276:immunology; D014162:Transfection; D014860:Warts / Q000276:immunology
chemical_list: D000941:Antigens; D000954:Antigens, Surface