Mutual interactions of the phosphate groups in locally deformed backbones of various DNA double helices at high salt concentrations.
Changes in the free energy of mutual phosphate group interactions are calculated that accompany bending of the A-, B- and Z-DNA backbones in 0.7, 2.1 and 4.2 mol/l NaCl aqueous solutions. The bending is often found to be favoured in the direction of the double helix grooves; B-DNA prefers bending into the major groove while minor groove is the preferred bending direction of A-DNA in the presence of 0.7 mol/l NaCl. Interestingly, the preferences are reversed in 4.2 mol/l NaCl. Further stabilization of A-DNA and B-DNA backbones is achieved in some cases if bending is combined with suitable local double helix twist alterations. Bending tendencies of Z-DNA backbone are generally weaker and they decrease, in contrast to B-DNA and A-DNA, with the increasing ionic strength.
Jursa
J
J
Institute of Biophysics, Czechoslovak Academy of Sciences, Brno.
Kypr
J
J
D004247:DNA / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D010710:Phosphates / Q000737:chemistry; D012965:Sodium Chloride; D012996:Solutions; D013816:Thermodynamics
D010710:Phosphates; D012996:Solutions; D012965:Sodium Chloride; D004247:DNA