[Expression of constitutive heterochromatin by AluI restriction endonuclease treatment and CA3/DA/DAPI staining of domestic pig chromosome].
The heterogeneity of constitutive heterochromatin in two domestic pigs has been studied by restriction endonuclease AluI banding, CA3/DA/DAPI banding and C-banding techniques. The results show that the centromere constitutive heterochromatin can be subdivided into three types, which located in different chromosomes. It is found that there is correspondence between No. 13--18 chromosomes in pigs and No. 1, 9, 16, Y chromosome in human by comparing the banding patterns of AluI and CA3/DA/DAPI. Therefore, it is revealed that satellite DNAs in constitutive heterochromatin of chromosomes mentioned above are similar.
Zhao
Y L
YL
Biology Department of Sichuan University, Chengdu.
Wang
X Z
XZ
Wang
Z S
ZS
Chen
W Y
WY
D000818:Animals; D002871:Chromosome Banding; D004247:DNA / Q000032:analysis*; D005260:Female; D006570:Heterochromatin / Q000737:chemistry*; D008297:Male; D013552:Swine / Q000235:genetics*
D006570:Heterochromatin; D004247:DNA