Effect of norspermidine and its related triamines on the cell-free polyphenylalanine synthesizing system from Vibrio parahaemolyticus.
The effect of norspermidine and its structurally related triamines on the cell-free polyphenylalanine synthesizing system from Vibrio parahaemolyticus was examined in connection with the requirement of the system for monovalent cation. In the absence of norspermidine, the maximal incorporation of [14C]phenylalanine into hot trichloroacetic acid insoluble material was observed under ionic conditions of 12 mM Mg2+ and 50 mM NH4+. K+ could partially substitute for NH4+, but Na+ could not. The addition of norspermidine to the polyphenylalanine synthetic reaction mixture not only lowered the optimal Mg2+ concentration, but it also stimulated the polyphenylalanine synthesis up to 2-fold with no significant increase in misincorporation of [14C]leucine. Other triamines having one or two methylene chains more than norspermidine were also effective in eliciting these effects. Furthermore, Na+ could not support the polyphenylalanine synthesis even in the presence of norspermidine and, on the contrary, inhibited the polyphenylalanine synthesis induced by NH4+ regardless of whether norspermidine was present or not. These findings are discussed in comparison with the properties of other bacterial cell-free systems.
Yamamoto
S
S
Faculty of Pharmaceutical Sciences, Okayma University.
Koumoto
Y
Y
Shikami
S
S
Shinoda
S
S
D000588:Amines / Q000008:administration & dosage / Q000494:pharmacology*; D000641:Ammonia / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007930:Leucine / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D010455:Peptides / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D013095:Spermidine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D014736:Vibrio parahaemolyticus / Q000187:drug effects*
D000588:Amines; D010455:Peptides; C012001:polyphenylalanine; C006760:norspermidine; D000641:Ammonia; D007930:Leucine; D008274:Magnesium; D011188:Potassium; D013095:Spermidine