The collagen-binding site of type-II units of bovine seminal fluid protein PDC-109 and fibronectin.
A single type-II domain has been isolated by limited proteolysis of the collagen-binding bovine seminal fluid protein, PDC-109. The 45-residue fragment corresponding to the second type-II domain of the parent molecule was found to have retained affinity for immobilized collagen, indicating that this minidomain carries critical regions of the collagen-binding site. Studies on various fragments of fibronectin have also implicated the two type-II units of this molecule in collagen-binding. In the present work we have found that type-II domains of human fibronectin, expressed in Escherichia coli as beta-galactosidase fusion proteins, bind specifically to immobilized collagen.
Bányai
L
L
Institute of Enzymology, Hungarian Academy of Sciences, Budapest.
Trexler
M
M
Koncz
S
S
Gyenes
M
M
Sipos
G
G
Patthy
L
L
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D003094:Collagen / Q000378:metabolism*; D005353:Fibronectins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D010957:Plasmids; D029584:Prostatic Secretory Proteins; D011487:Protein Conformation; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification / Q000378:metabolism; D015183:Restriction Mapping; D012661:Semen / Q000378:metabolism; D029589:Seminal Plasma Proteins
D005353:Fibronectins; D029584:Prostatic Secretory Proteins; D011506:Proteins; D011993:Recombinant Fusion Proteins; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein; D003094:Collagen