Enhanced labeling of mitotic retinal cells with an intraocular [3H]thymidine injection.
Tritiated thymidine ([3H]Tdr) was injected either intraocularly (i.o.) or intraperitoneally (i.p.). Autoradiography showed that more retinal cells were labeled by i.o. than by i.p. injection. Controls showed that the increased number of labeled retinal cells was not related to glioblast proliferation produced by trauma from the i.o. injection. After injection of identical doses, the peak concentration of [3H]Tdr available for incorporation into DNA was about 100 times greater after an i.o. than after an i.p. injection. The effective duration of the labeling pulse in goldfish was 9 times longer for an i.o. than an i.p. injection. This study shows that i.o. delivery of [3H]Tdr is preferable to i.p. injection for efficient delivery of label to proliferating retinal cells.
Springer
A D
AD
Department of Cell Biology and Anatomy, New York Medical College, Valhalla 10595.
Sabban
E L
EL
Morel
K D
KD
Berk
M F
MF
Vogel
J R
JR
Wilson
B R
BR
D000818:Animals; D001345:Autoradiography; D005123:Eye; D005724:Ganglia / Q000166:cytology / Q000502:physiology; D006054:Goldfish; D007267:Injections; D007274:Injections, Intraperitoneal; D008938:Mitosis; D012160:Retina / Q000166:cytology*; D013936:Thymidine
D013936:Thymidine