Studies on acetylcholine sensor and its analytical application based on the inhibition of cholinesterase.
Acetylcholine esterase electrodes, based on glass, Pd/PdO and Ir/IrO2 electrodes as pH sensor, using the immobilized acetylcholine esterase in acrylamide-methacrylamide hydrazides prepolymer are reported and compared. New data on the analysis of nicotine, fluoride ion, and some organophosphorus compounds are reported using the present AChE sensor based on the inhibition of the immobilized acetylcholine esterase. Reactivation of immobilized AChE after inhibition with reversible inhibitor, i.e. nicotine and fluoride ion is carried out using a mixture of working buffer and acetylcholine, whereas reactivation after inhibition with irreversible inhibitor, i.e. organophosphorus compounds is carried out using a mixture of acetylcholine and pyridine-2-aldoxime methiodide (PAM). The detection limits for the nicotine and fluoride ion are found to be 10(-5) M whereas for paraoxon, methyl parathion and malathion are found to be 10(-9) M and 10(-10) M.
Tran-Minh
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C
Laboratoire de Biotechnologie, Ecole des Mines, Saint-Etienne, France.
Pandey
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PC
Kumaran
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S
D000109:Acetylcholine; D015374:Biosensing Techniques; D002800:Cholinesterase Inhibitors; D005459:Fluorides / Q000032:analysis; D008294:Malathion / Q000032:analysis; D008743:Methyl Parathion / Q000032:analysis; D009538:Nicotine / Q000032:analysis; D010261:Paraoxon / Q000032:analysis
D002800:Cholinesterase Inhibitors; D008743:Methyl Parathion; D009538:Nicotine; D000109:Acetylcholine; D005459:Fluorides; D010261:Paraoxon; D008294:Malathion