Combined GTG-banding and nonradioactive in situ hybridization improves characterization of complex karyotypes.
Nonradioactive in situ hybridization (ISH) using biotinylated centromere probes for chromosomes 1, 6, 7, 10, 16, 17, 18, and the X, respectively, was combined with GTG-banding to study cytogenetic changes in two different ovarian cancer cell lines. ISH was performed after GTG-banding on the same metaphase. The use of a low trypsin concentration (0.01%) in the banding procedure was essential for subsequent ISH. This combined approach allows the detection of subtle chromosomal rearrangements and appears to aid the identification of marker chromosomes.
Smit
V T
VT
Department of Pathology, Leiden University The Netherlands.
Wessels
J W
JW
Mollevanger
P
P
Schrier
P I
PI
Raap
A K
AK
Beverstock
G C
GC
Cornelisse
C J
CJ
D000328:Adult; D002478:Cells, Cultured; D002503:Centromere; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D015342:DNA Probes; D005260:Female; D006801:Humans; D007621:Karyotyping / Q000379:methods*; D008297:Male; D009693:Nucleic Acid Hybridization; D010051:Ovarian Neoplasms / Q000235:genetics*; D014357:Trypsin / Q000378:metabolism; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D014357:Trypsin