Separation of drug-loaded nanoparticles from free drug by gel filtration.
A suitable method for separating free drug and other residual compounds from drug-loaded, colloidal polymeric particles was developed. The objective of this separation procedure was to purify particle suspensions on a preparative scale by a rapid, simple, and exact method without changing the properties of the particles. The gel filtration method described in this paper using a middle-pressure chromatographic system is able to meet all these requirements. The separation process could be optimized by using only water as an eluent in combination with Sephadex G 50 medium. The purified particles appeared after 12-13 min in the eluate. Neither dispersity nor particle size or loading rate were influenced to a considerable extent.
Beck
P
P
Department of Pharmaceutical Technology, J. W. Goethe-University Frankfurt, FRG.
Scherer
D
D
Kreuter
J
J
D002214:Capsules; D002850:Chromatography, Gel; D003487:Cyanoacrylates; D004339:Drug Compounding / Q000379:methods*; D004659:Enbucrilate; D010316:Particle Size; D004364:Pharmaceutical Preparations / Q000008:administration & dosage / Q000302:isolation & purification*; D011108:Polymers
D002214:Capsules; D003487:Cyanoacrylates; D004364:Pharmaceutical Preparations; D011108:Polymers; D004659:Enbucrilate