Protein extracts for nutritional purposes from fragile strains of Saccharomyces cerevisiae: construction of strains and conditions for lysis.
A superfragile strain, Saccharomyces cerevisiae 211, carrying three nonallelic nuclear determinants of cell lysis by osmotic shock was isolated from the haploid progeny of a cross between two laboratory fragile strains VY 1160 and SY 15. The strain Sacch. cerevisiae 211 is prototrophic, grows well in both laboratory and industrial media, only when supplemented with osmotic stabilizers. The average protein content of its biomass after growth in a molasses-based nutritional medium is 48.7%. In laboratory conditions this strain releases about 60% of its cellular proteins by spontaneous lysis in water. However, on a pilot scale the protein yield decreases to about 25%. After a single disintegration step at 650 atm, practically all of the cellular protein--91.5%, is extracted without any additional chemical treatment. This result cannot be reached in any of the wild type strains tested even after triple treatment at 650 atm.
Stateva
L
L
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
Venkov
P
P
Lyutskanov
N
N
Koleva
L
L
Hadjiolov
A
A
D004044:Dietary Proteins; D005656:Fungal Proteins / Q000302:isolation & purification*; D009154:Mutation; D009753:Nutritive Value; D009997:Osmotic Pressure; D012441:Saccharomyces cerevisiae / Q000032:analysis* / Q000235:genetics / Q000378:metabolism
D004044:Dietary Proteins; D005656:Fungal Proteins; C012551:single cell proteins