Human macrophage scavenger receptors: primary structure, expression, and localization in atherosclerotic lesions.
Two types of cDNAs for human macrophage scavenger receptors were cloned from a cDNA library derived from the phorbol ester-treated human monocytic cell line THP-1. The type I and type II human scavenger receptors encoded by these cDNAs are homologous (73% and 71% amino acid identity) to their previously characterized bovine counterparts and consist of six domains: cytoplasmic (I), membrane-spanning (II), spacer (III), alpha-helical coiled-coil (IV), collagen-like (V), and a type-specific C-terminal (VI). The receptor gene is located on human chromosome 8. The human receptors expressed in CHO-K1 cells mediated endocytosis of modified low density lipoproteins. Two mRNAs, 4.0 and 3.2 kilobases, have been detected in human liver, placenta, and brain. Immunohistochemical studies using an anti-peptide antibody which recognizes human scavenger receptors indicated the presence of the scavenger receptors in the macrophages of lipid-rich atherosclerotic lesions, suggesting the involvement of scavenger receptors in atherogenesis.
Matsumoto
A
A
Third Department of Internal Medicine, University of Tokyo, Japan.
Naito
M
M
Itakura
H
H
Ikemoto
S
S
Asaoka
H
H
Hayakawa
I
I
Kanamori
H
H
Aburatani
H
H
Takaku
F
F
Suzuki
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001011:Aorta / Q000276:immunology / Q000473:pathology; D001161:Arteriosclerosis / Q000276:immunology* / Q000473:pathology; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D015723:Gene Library; D006801:Humans; D008264:Macrophages / Q000276:immunology*; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000276:immunology / Q000473:pathology; D010455:Peptides / Q000138:chemical synthesis; D016133:Polymerase Chain Reaction; D011971:Receptors, Immunologic / Q000032:analysis / Q000235:genetics*; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; D051117:Scavenger Receptors, Class A; D051122:Scavenger Receptors, Class B; D012689:Sequence Homology, Nucleic Acid; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014162:Transfection
C495139:MSR1 protein, human; D008565:Membrane Proteins; D010455:Peptides; D011971:Receptors, Immunologic; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; C454739:Scarb1 protein, mouse; D051117:Scavenger Receptors, Class A; D051122:Scavenger Receptors, Class B; D004247:DNA; D013755:Tetradecanoylphorbol Acetate