[Result of "as is patch test" using mite elements in atopic dermatitis (AD) patient. 2nd report, "Mite lipid"].
The antigenicity of mite lipid was studied, taking atopic dermatitis as an allergic disease. Lipid was extracted from Dermatophagoides pteronyssinus (Dp) and D. farinae (Df). It was tentatively designated a mite lipid antigen (Dp-L, Df-L), and then a patch test was performed. An extract from animal food (LC) and acetone were used as the controls. With only Dp-L, a positive reaction of more than one plus (+) of the ICDRG standard was noted in 3 out of 21 cases of AD. Histology of the above-mentioned positive cases revealed spongiosis and infiltration of small round cells in the upper dermis along with scattered eosinophiles. Leu 6 positive cells were noted frequently in the epidermis and dermis. The lipid components of Dp-L were made up mostly of phospholipid and triglycerides+, and lipoprotein was not contained. Judging from the findings above it appears necessary to study not only protein but also lipid in connection with the mite antigenicity++ in AD in the future.
Sakurai
M
M
Department of Dermatorogy, Saiseikai Central Hospital.
Nakayama
H
H
Kumei
A
A
Tsurumachi
K
K
Takaoka
M
M
D000293:Adolescent; D000328:Adult; D000818:Animals; D000941:Antigens / Q000276:immunology* / Q000302:isolation & purification; D002648:Child; D003876:Dermatitis, Atopic / Q000276:immunology* / Q000473:pathology; D005260:Female; D006801:Humans; D008055:Lipids / Q000276:immunology* / Q000302:isolation & purification; D008297:Male; D008925:Mites / Q000032:analysis / Q000276:immunology*; D010328:Patch Tests; D012867:Skin / Q000276:immunology* / Q000473:pathology
D000941:Antigens; D008055:Lipids