[Detection of toxigenic Vibrio cholerae O1 using polymerase chain reaction for amplifying the cholera enterotoxin gene].
A rapid and simple procedure using polymerase chain reaction (PCR) was developed for the detection of cholera enterotoxin (CT) producing character. This method is based on amplifying a 380 base pair (bp) segment of the CT gene (ctx) which controls the production of CT. Two single-stranded oligonucleotides, synthetized to be complementary to the known nucleotide sequences of genes encoding the A-subunit of ctx, were used as extension primers. The oligonucleotide sequences are 5'TCAAACTATATTGTCTGGTC (CT-1) and 5'CGCAAGTATTACTCATCGA (CT-2). As template DNA was used 5 microliter of boiled bacterial culture broth at 95 degrees C for 5 min without the need for DNA extraction. The amplified target DNA were confirmed with only CT producing Vibrio cholerae O1 but not with CT non-producing organisms such as heat labile enterotoxin producing Escherichia coli by electrophoretic analysis of PCR mixture after amplification. A few isolates of CT producing V. mimicus and V. cholerae non-O1 were identified.
Kobayashi
K
K
Division of Public Health, Osaka Prefectural Institute of Public Health.
Seto
K
K
Akasaka
S
S
Makino
M
M
D004768:Enterotoxins / Q000235:genetics*; D005784:Gene Amplification; D005798:Genes, Bacterial; D016133:Polymerase Chain Reaction; D014734:Vibrio cholerae / Q000235:genetics / Q000302:isolation & purification*
D004768:Enterotoxins; C037420:stN protein, Vibrio cholerae