The transition of the higher-order structure of rat liver chromatin takes place at about 85% neutralization of DNA charges.
The salt dependence of the stoichiometry in the binding of linker histone to rat liver chromatin was analysed. It was found that the transition of the higher-order structure of chromatin (between folded and unfolded) takes place at the point of approximately 85% neutralization of DNA charges in chromatin, as in a related process in the B-Z transition of double-helical DNA.
Watanabe
F
F
Institut für Zellbiologie, ETH Hönggerberg Zürich.
D000818:Animals; D002843:Chromatin / Q000737:chemistry* / Q000378:metabolism; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000737:chemistry* / Q000378:metabolism; D009500:Neutralization Tests; D009690:Nucleic Acid Conformation; D051381:Rats
D002843:Chromatin; D006657:Histones; D004247:DNA