title: Biochemical characterization, localization and immunostimulating properties of a soluble glycoprotein, Ag1, isolated from in vitro cultures of Plasmodium falciparum.
abstract: The soluble amphiphilic glycoprotein, Ag1 (gp60), purified from supernatants of in vitro cultures of Plasmodium falciparum has a molecular mass of 60 kDa and did not exhibit size variation in the different P. falciparum isolates tested by immunoblotting. Ag1 was shown to interact with the lectin Erythrina christagalli agglutinin, which is specific for carbohydrates bearing beta-D-galactose(1-4)-D-N-acetylglucosamine. Indirect immunofluorescence studies showed that Ag1 is located on the surface of trophozoites and schizonts but not on the surface of merozoites. Ag1 is recognized by human immune sera from six different malaria-endemic regions. Ag1 induces in vitro proliferation of lymphocytes from malaria-immune individuals in an antigen-specific manner.
authors: Department of Treponematoses, State Seruminstitute, Copenhagen, Denmark.
authors: P H
authors: PH
authors: Jakobsen
authors: S
authors: S
authors: Jepsen
authors: E M
authors: EM
authors: Riley
authors: T G
authors: TG
authors: Theander
authors: P
authors: P
authors: Grellier
authors: A
authors: A
authors: Lihme
authors: L
authors: L
authors: Hviid
authors: M
authors: M
authors: Dziegiel
authors: J
authors: J
authors: Schrevel
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis / Q000737:chemistry / Q000276:immunology*; D000954:Antigens, Surface / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000032:analysis / Q000737:chemistry / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D007123:Immunoelectrophoresis, Two-Dimensional; D008213:Lymphocyte Activation; D008288:Malaria / Q000276:immunology; D010963:Plasmodium falciparum / Q000276:immunology*; D011233:Precipitin Tests; D015800:Protozoan Proteins / Q000032:analysis / Q000737:chemistry / Q000276:immunology
chemical_list: D000953:Antigens, Protozoan; D000954:Antigens, Surface; D006023:Glycoproteins; D007106:Immune Sera; D015800:Protozoan Proteins