Pharmacokinetics of physostigmine intramuscularly administered to guinea pigs.
Physostigmine pharmacokinetics was determined in guinea pigs following im administration of 5-146 micrograms/kg. Eighteen male guinea pigs were divided into three equal groups and given dosages of 5, 27, and 146 micrograms/kg, respectively. Physostigmine was given in the right hind limb and blood samples were collected at various times up to 300 min postinjection via an indwelling carotid catheter. Unbound physostigmine plasma concentrations were analyzed by HPLC. The concentration-time profile for each animal was fitted to standard pharmacokinetic models. A one-compartment open model with first-order absorption and elimination provided the best fit. For all dosage groups, physostigmine concentrations peaked in approximately 30 min. Apparent volumes of distribution (assuming 100% bioavailability) ranged from 1.9 to 2.2 L/kg. Systemic clearances and elimination half-lives were 30-36 mL/min/kg and 40-50 min, respectively. The area under the concentration-time curve and the Cmax were linearly related to the dose, indicating pharmacokinetic linearity. In conclusion, physostigmine, intramuscularly administered to the guinea pig, is absorbed, distributed, and eliminated rapidly, and the pharmacokinetics behave linearly within the 5-146-micrograms/kg dosage range.
Lukey
B J
BJ
Aberdeen Proving Ground, MD 21010-5425.
Parrish
J H
JH
Marlow
D D
DD
Clark
C R
CR
Sidell
F R
FR
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006168:Guinea Pigs; D007273:Injections, Intramuscular; D008297:Male; D008954:Models, Biological; D010830:Physostigmine / Q000008:administration & dosage / Q000493:pharmacokinetics*
D010830:Physostigmine