Inhibition of the dimorphic transition of Candida albicans by the ornithine decarboxylase inhibitor 1,4-diaminobutanone: alterations in the glycoprotein composition of the cell wall.
Hyphal development in Candida albicans was selectively blocked by the ornithine decarboxylase competitive inhibitor 1,4-diaminobutanone (DAB). Inhibition of hyphal development required DAB during both yeast inoculum growth and subsequent incubation at 37 degrees C to induce mycelial growth. This effect was not due to general growth inhibition since DAB did not inhibit yeast growth, and reduced protein synthesis by 30% at most. Moreover, protein synthesis was unaffected by DAB when cells were pre-grown in drug-containing media. Since DAB inhibited dimorphic transition at 37 degrees C, morphology- and temperature-dependent protein synthesis could be distinguished. DAB stimulated the synthesis of several yeast wall-proteins, irrespective of morphology or growth temperature, and two at 37 degrees C only, but it inhibited the synthesis of a single mycelial-specific glycoprotein species.
Martinez
J P
JP
Departament de Microbiologia, Facultat de Farmacia, Universitat de Valencia, Spain.
Lopez-Ribot
J L
JL
Gil
M L
ML
Sentandreu
R
R
Ruiz-Herrera
J
J
D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism; D005656:Fungal Proteins / Q000096:biosynthesis; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D065108:Ornithine Decarboxylase Inhibitors; D011700:Putrescine / Q000031:analogs & derivatives* / Q000494:pharmacology
D005656:Fungal Proteins; D008562:Membrane Glycoproteins; D065108:Ornithine Decarboxylase Inhibitors; C025363:mannoproteins; C014458:1,4-diaminobutanone; D011700:Putrescine