Recent advances in capillary electrophoresis of proteins, peptides and amino acids.
The current status of high-performance capillary electrophoresis as an analytical separation method for proteins, peptides and amino acids is assessed. Recent advances in suppressing the effects of electroosmotic flow and irreversible adsorption of proteins at the capillary wall are reviewed, together with procedures for optimal separations of peptides and amino acids. The detection aspects emphasize the role of laser-induced fluorescence and capillary electrophoresis/mass spectrometry in high-sensitivity measurements.
Novotny
M V
MV
Department of Chemistry, Indiana University, Bloomington 47405.
Cobb
K A
KA
Liu
J P
JP
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000302:isolation & purification*; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010455:Peptides / Q000302:isolation & purification*; D011506:Proteins / Q000302:isolation & purification*
D000596:Amino Acids; D010455:Peptides; D011506:Proteins