title: An extended QBASIC program for the normalization and computation of whole-cell protein profiles and the application to clinically important Candida species.
abstract: The computer program described enables a rapid calculation of relative molecular masses of proteins from different yeasts and microbial pathogens, by interpolation from a molecular weight calibration curve that comprises stepwise linear regression between the protein bands produced by the internal standards. A similarity matrix can then be produced, taking into account variations between calculated molecular masses caused by small differences in bandwidths and/or positions of specific protein bands. This program has been applied to different Candida species and the similarity data obtained further analysed numerically utilizing CLUSTAN II on a SPERRY 1100 multi-processor.
authors: Department of Dermatology, Medical Faculty, University of the Orange Free State, Bloemfontein, Republic of South Africa.
authors: E J
authors: EJ
authors: Smit
authors: P W
authors: PW
authors: Van Wyk
authors: J P
authors: JP
authors: vd Westhuizen
authors: J L
authors: JL
authors: Kock
mesh_terms: D015373:Bacterial Typing Techniques; D002175:Candida / Q000032:analysis* / Q000145:classification; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000737:chemistry*; D006801:Humans; D008838:Microcomputers; D008970:Molecular Weight; D012044:Regression Analysis; D012984:Software
chemical_list: D005656:Fungal Proteins