Quantitative analysis of cytoskeletal proteins throughout the cell cycle of the MRC-5 fibroblastic cell line.
Fluorescent dyes were used to stain actin, vimentin, tubulin and DNA in the same MRC-5 fibroblastic cells. Cytofluorometry and image analysis were then used to quantitatively evaluate the F actin, vimentin and tubulin content throughout the cell cycle. The results showed that different cells can have the same DNA content while their cytoskeletal protein content is variable. The data also showed that cytoskeletal protein content variations exist throughout the cell cycle of the fibroblastic cell line. The F actin content increased during the cell cycle from G1 to G2 phases and decreased in M phase. The amount of tubulin in the G2 was about twice as much as that in the G1 phase, before decreasing in the M phase; there was a threshold of tubulin content for G2 cells entering S phase.
Léger
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I
Image Processing and Quantitative Microscopy Laboratory (UA CNRS 397), Joseph Fourier University, Grenoble, France.
Giroud
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F
Brugal
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G
D000199:Actins / Q000378:metabolism; D000918:Antibody Specificity; D002453:Cell Cycle / Q000502:physiology*; D002460:Cell Line; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D005456:Fluorescent Dyes; D006801:Humans; D008856:Microscopy, Fluorescence; D014404:Tubulin / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
D000199:Actins; D003598:Cytoskeletal Proteins; D005456:Fluorescent Dyes; D014404:Tubulin; D014746:Vimentin; D004247:DNA