Biosynthesis and properties of a further member of the small chondroitin/dermatan sulfate proteoglycan family.
Human osteosarcoma cells express a 78-kDa proteoglycan core protein to which an asparagine-bound oligosaccharide, O-glycosidically linked oligosaccharides and probably only a single chondroitin 6-sulfate chain of 29-kDa are bound. Prior to O-glycosylation, the N-glycosylated core protein exhibits a mass of 83 kDa. Upon digestion of the secreted proteoglycan with chondroitin ABC lyase a mature core protein with an apparent molecular mass of 106 kDa is obtained. Smaller amounts of core proteins of 101 and 115 kDa can be detected occasionally. The glycosaminoglycan composition and the relative molecular mass of the glycosaminoglycan chain distinguish this proteoglycan, tentatively named proteoglycan 100 (PG-100), from biglycan (small proteoglycan I) and decorin (small proteoglycan II) which are also expressed by osteosarcoma cells. An antiserum against PG-100 shows partial cross-reactivity with decorin, but in contrast to the latter proteoglycan it does not bind to type I collagen fibrils. PG-100 is not a unique product of osteosarcoma cells. It has also been found in the secretions of human skin fibroblasts.
Schwarz
K
K
Institute of Physiological Chemistry and Pathobiochemistry, University of Munster, Federal Republic of Germany.
Breuer
B
B
Kresse
H
H
D011508:Chondroitin Sulfate Proteoglycans / Q000096:biosynthesis* / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D058575:Decorin; D016326:Extracellular Matrix Proteins; D006025:Glycosaminoglycans / Q000737:chemistry; D006027:Glycosides / Q000494:pharmacology; D006801:Humans; D007158:Immunologic Techniques; D066298:In Vitro Techniques; D008970:Molecular Weight; D012516:Osteosarcoma / Q000378:metabolism*; D011485:Protein Binding; D011509:Proteoglycans / Q000096:biosynthesis; D012867:Skin / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014415:Tunicamycin / Q000494:pharmacology
D011508:Chondroitin Sulfate Proteoglycans; C546878:DCN protein, human; D058575:Decorin; D016326:Extracellular Matrix Proteins; D006025:Glycosaminoglycans; D006027:Glycosides; D011509:Proteoglycans; D014415:Tunicamycin; C008646:4-nitrophenyl beta-D-xyloside; D003094:Collagen