An extended QBASIC program for the normalization and computation of whole-cell protein profiles and the application to clinically important Candida species.
The computer program described enables a rapid calculation of relative molecular masses of proteins from different yeasts and microbial pathogens, by interpolation from a molecular weight calibration curve that comprises stepwise linear regression between the protein bands produced by the internal standards. A similarity matrix can then be produced, taking into account variations between calculated molecular masses caused by small differences in bandwidths and/or positions of specific protein bands. This program has been applied to different Candida species and the similarity data obtained further analysed numerically utilizing CLUSTAN II on a SPERRY 1100 multi-processor.
Smit
E J
EJ
Department of Dermatology, Medical Faculty, University of the Orange Free State, Bloemfontein, Republic of South Africa.
Van Wyk
P W
PW
vd Westhuizen
J P
JP
Kock
J L
JL
D015373:Bacterial Typing Techniques; D002175:Candida / Q000032:analysis* / Q000145:classification; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000737:chemistry*; D006801:Humans; D008838:Microcomputers; D008970:Molecular Weight; D012044:Regression Analysis; D012984:Software
D005656:Fungal Proteins