AUUUA motifs are dispensable for rapid degradation of the mouse c-myc RNA.
Sequence determinants responsible for c-myc RNA rapid turn-over are localized within the 3' non-coding region which is mainly characterized by the presence of two polyadenylation signals and a high content in A and U. Although the AUUUA/UUAUUUA motif is commonly thought to specify a whole class of unstable RNAs coding for various onco-proteins and cytokines, site-directed mutagenesis showed that both of the two such sequences found in the mouse c-myc RNA are dispensable for rapid RNA degradation. Although less efficient than the whole 3' non-coding region, the last 50 nucleotides of c-myc RNA, mainly made up of U and A and devoid of AUUUA/UUAUUUA motif, are sufficient to confer instability to the coding sequence.
Bonnieu
A
A
Laboratoire de Biologie Moléculaire URA CNRS 1191 Génétique Moléculaire, USTL, Montpellier, France.
Roux
P
P
Marty
L
L
Jeanteur
P
P
Piechaczyk
M
M
D000818:Animals; D001483:Base Sequence; D016259:Genes, myc; D006801:Humans; D007700:Kinetics; D007739:L Cells / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D015345:Oligonucleotide Probes; D012333:RNA, Messenger