IgG-dependent generation of platelet-activating factor by normal and low density human eosinophils.
We have compared normal and low density human eosinophils for their ability to generate platelet activating factor (PAF) in response to IgG-dependent and nonimmunologic stimulation. After 45 min incubation with IgG-coated Sepharose beads the concentrations of cell-associated PAF recovered from normal density eosinophils were significantly greater than from low-density eosinophils or neutrophils. Moreover, eosinophils stimulated with calcium ionophore A23187 had a considerably greater capacity to generate PAF than had previously been described. Although the quantities of cell-associated PAF recovered from normal and low density eosinophils and neutrophils after A23187 stimulation were similar, the amounts of extracellular PAF recovered from both eosinophil populations were significantly greater than from neutrophils. The amounts of PAF recovered from the low density eosinophils may not reflect the full synthetic capacity of these cells, because PAF-turnover was found to be more rapid than that observed with normal density eosinophils. When exogenous [3H]PAF was added to the two stimulated eosinophil populations subsequent analysis of the [3H]PAF metabolites by DIOL-HPLC revealed that low density eosinophils incorporated PAF into the phosphatidylcholine (PC) pool more rapidly than did normal density eosinophils or neutrophils. Alkaline hydrolysis of the PC fraction from whole cell extracts followed by treatment with acetic anhydride resulted in all the PC-associated radioactivity being converted to [3H]PAF, confirming PAF incorporation to PC via this pathway. These findings suggest that the contribution of eosinophils to inflammatory processes through the generation of PAF may be greater than previously appreciated, and that Ig-mediated stimulation may be important in initiating generation of the mediator. Low density eosinophils, that are presumed to be similar to tissue eosinophils, may have a role in regulating PAF concentrations in tissues through their enhanced rate of metabolism.
Cromwell
O
O
Department of Allergy and Clinical Immunology, National Heart and Lung Institute, London, UK.
Wardlaw
A J
AJ
Champion
A
A
Moqbel
R
R
Osei
D
D
Kay
A B
AB
D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D004804:Eosinophils / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000502:physiology*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000096:biosynthesis*; D010974:Platelet Aggregation; D013189:SRS-A / Q000096:biosynthesis
D007074:Immunoglobulin G; D010972:Platelet Activating Factor; D013189:SRS-A; D000001:Calcimycin; D009240:N-Formylmethionine Leucyl-Phenylalanine