T cells from nonresponder mice. MHC restricted and unrestricted recognition of insulin.
Two types of insulin-reactive T cell hybridomas expressing TCR-alpha beta were derived from nonresponder H-2b mice immunized with pork insulin. One type had characteristics of conventional class II-restricted Th cells. These CD4+ CD8- I-Ab-restricted T cells recognized a self determinant, present within the insulin B-chain. This determinant was distinct from the immunodominant A-chain loop determinant that is recognized by the majority of T cells induced after immunization with normally immunogenic beef insulin. Our results suggest that this determinant is readily generated during immunologic processing of insulins, including nonimmunogenic pork insulin and self insulin. A second type of T cell lacking CD4 and CD8 recognized a distinct B-chain determinant of insulin in a class II-dependent, but MHC unrestricted, fashion. These cells may represent a novel subpopulation which has bypassed conventional selection during development in the thymus.
Jensen
P E
PE
Department of Pathology, Emory University School of Medicine, Atlanta, GA 30322.
Kraft
A O
AO
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D002417:Cattle; D002462:Cell Membrane / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007328:Insulin / Q000276:immunology*; D008213:Lymphocyte Activation; D008285:Major Histocompatibility Complex; D051379:Mice; D008815:Mice, Inbred Strains / Q000276:immunology; D051381:Rats; D012756:Sheep; D013045:Species Specificity; D013552:Swine; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000945:Antigens, Differentiation, T-Lymphocyte; D000949:Histocompatibility Antigens Class II; D007328:Insulin