Determination of human serum phospholipase A2. Comparison of two methods.
A photometric assay for serum phospholipase A is compared with a radiometric assay specific for phospholipase A2. The methods show a high correlation. The increased phospholipase A activity in sera of patients with inflammatory and non-pancreatic necrotizing diseases is attributable to a neutral-active, calcium-sensitive phospholipase A2 but not to pancreatic phospholipase A2. Phospholipase A1 did not seem to contribute significantly to the overall activity of phospholipase A.
Märki
F
F
Ciba-Geigy AG, Research Department, Basel, Switzerland.
Pignat
W
W
Steinbrückner
B
B
Hoffmann
G E
GE
D005069:Evaluation Studies as Topic; D006801:Humans; D007249:Inflammation / Q000097:blood; D010741:Phospholipases A / Q000097:blood*; D054466:Phospholipases A1; D054467:Phospholipases A2; D010783:Photometry / Q000379:methods; D011874:Radiometry / Q000379:methods
D010741:Phospholipases A; D054466:Phospholipases A1; D054467:Phospholipases A2