Mucocutaneous leishmaniasis due to Leishmania (Leishmania) infantum: biochemical characterization.
A vegetative mass in the right nasal cavity of a 62-year-old man from Palma de Mallorca, Spain, was found to be due to Leishmania. The organism was isolated in culture and characterized by in situ hybridization, Southern blot hybridization, and isoenzyme analysis; it was thus demonstrated to be the most common enzyme variant 1 (MON 1) of Leishmania (Leishmania) infantum.
Alvar
J
J
Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Madrid, Spain.
Ballesteros
J A
JA
Soler
R
R
Benito
A
A
van Eys
G J
GJ
Schoone
G J
GJ
Cabrer
B
B
D000818:Animals; D015139:Blotting, Southern; D016054:DNA, Protozoan / Q000032:analysis; D004592:Electrophoresis, Starch Gel; D006801:Humans; D007527:Isoenzymes / Q000032:analysis; D007893:Leishmania donovani / Q000032:analysis / Q000201:enzymology* / Q000302:isolation & purification; D007897:Leishmaniasis, Mucocutaneous / Q000469:parasitology*; D008297:Male; D008875:Middle Aged; D009296:Nasal Cavity / Q000469:parasitology*; D009668:Nose Diseases / Q000469:parasitology; D009693:Nucleic Acid Hybridization; D013030:Spain
D016054:DNA, Protozoan; D007527:Isoenzymes