[In situ hybridization on tissue section: a new method for studying the amacrine cells of the retina and the centers of the visual system].
In situ hybridization of nucleic acid probes, encoding the key enzymes of the synthesis of neurotransmitters, with sections of retina is a new method giving complementary informations to the data obtained by immunocytochemistry and autoradiography. It will allow a better characterization of amacrin cells, whatever might be their type, and a refined understanding of their function. It is an accurate tool to investigate the mechanisms of retinal degenerations and spontaneous or experimental microphtalmia.
Abitbol
M
M
Laboratoire de Neurobiologie Cellulaire et Moléculaire, CNRS, Gif-sur-Yvette.
Dumas
S
S
Julien
J F
JF
Rhyner
T A
TA
Dufier
J L
JL
Mallet
J
J
D000818:Animals; D001921:Brain / Q000166:cytology*; D001923:Brain Chemistry; D008297:Male; D008722:Methods; D009693:Nucleic Acid Hybridization; D051381:Rats; D011919:Rats, Inbred Strains; D012160:Retina / Q000737:chemistry* / Q000166:cytology*
L'hybridation in situ sur coupe de tissu (HIS): une nouvelle méthode d'étude des cellules amacrines de la rétine et des centres du système visuel.