Effects of metal ions on 15-hydroxy prostaglandin dehydrogenase activity in rabbit kidney cortex.
The effects of Cu2+, Fe2+ and Zn2+ on 15-hydroxy prostaglandin dehydrogenase activity in rabbit kidney cortex were examined. Cu2+ and Zn2+ (0.05-0.5 mM) inhibited the activity of this enzyme in a dose-dependent manner. The concentration required for 50% inhibition was approximately 0.1 mM for Cu2+ and 0.15 mM for Zn2+. The inhibition by both metals was uncompetitive and non-competitive with regard to NAD+ and prostaglandin E2, respectively, indicating that the mechanisms of the inhibition on the enzyme of both metals may be the same. Fe2+ had no effect on the activity of this enzyme. These results suggest that Cu2+ and Zn2+ have the potential to modulate the catabolism of prostaglandins by the kidney cortex.
Sakuma
S
S
Department of Hygienic Chemistry, Osaka University of Pharmaceutical Sciences, Japan.
Fujimoto
Y
Y
Hikita
E
E
Okano
Y
Y
Yamamoto
I
I
Fujita
T
T
D000818:Animals; D002413:Cations, Divalent; D003300:Copper / Q000494:pharmacology*; D006911:Hydroxyprostaglandin Dehydrogenases / Q000378:metabolism*; D007501:Iron / Q000494:pharmacology*; D007672:Kidney Cortex / Q000201:enzymology*; D007700:Kinetics; D008297:Male; D011817:Rabbits; D015032:Zinc / Q000494:pharmacology*
D002413:Cations, Divalent; D003300:Copper; D007501:Iron; D006911:Hydroxyprostaglandin Dehydrogenases; C035859:15-hydroxyprostaglandin dehydrogenase; D015032:Zinc