title: The first phase insulin response to intravenous glucose is highly reproducible.
abstract: To determine the reproducibility of the first phase insulin response to intravenous glucose, ten normal subjects underwent two intravenous glucose tolerance tests separated by at least two weeks. Intravenous dextrose (0.3 g/kg) was administered over 2 min by continuous infusion and arterialised-venous samples were taken from a retrogradely cannulated hand vein in the opposite arm. Within subjects, median coefficient of variation for the 3 min insulin was 4.0% (range 1.2-24.3%) and median coefficient of variation for the 0-10 min area was 6.7% (range 1.7-18.8%). These coefficients of variation are close to those of the assay itself (less than 10%). Despite this, between subject responses varied by greater than sixfold. In conclusion, contrary to previous reports the intravenous glucose tolerance test is highly reproducible. This makes it a very valuable tool for further studies of the pathogenesis of diabetes.
authors: Department of Clinical Biochemistry, Addenbrooke's Hospital, Cambridge, UK.
authors: G
authors: G
authors: Rayman
authors: P
authors: P
authors: Clark
authors: A E
authors: AE
authors: Schneider
authors: C N
authors: CN
authors: Hales
mesh_terms: D000328:Adult; D005260:Female; D005947:Glucose / Q000008:administration & dosage / Q000494:pharmacology*; D005951:Glucose Tolerance Test; D006801:Humans; D007262:Infusions, Intravenous; D007328:Insulin / Q000097:blood / Q000378:metabolism*; D000078790:Insulin Secretion; D008297:Male; D015203:Reproducibility of Results
chemical_list: D007328:Insulin; D005947:Glucose