Endotoxin releases platelet-activating factor from human monocytes in vitro.
The role of platelet-activating factor (PAF) in human endotoxaemia was investigated by incubating human monocytes from 27 subjects with S. minnesota endotoxin in vitro. Monocytes synthesized 1.01 +/- 0.4 x 10(-10) M to 5.3 +/- 1.51 x 10(-9) M PAF after 60 min of incubation with endotoxin at concentrations of 1 x 10(-8) M and 4.5 x 10(-6) M respectively. The endotoxin-stimulated release of PAF was not significantly increased in monocytes cultured on a human endothelial cell monolayer. This rapid release of PAF in response to endotoxin is consistent with a role for monocyte-derived PAF as a toxic mediator of the acute systemic changes observed in patients with endotoxin-related septic shock.
Leaver
H A
HA
Department of Pharmacology, University of Edinburgh, Scotland.
Qu
J M
JM
Smith
G
G
Howei
A
A
Ross
W B
WB
Yap
P L
PL
D002478:Cells, Cultured; D003470:Culture Media; D004731:Endotoxins / Q000633:toxicity*; D006801:Humans; D066298:In Vitro Techniques; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D010972:Platelet Activating Factor / Q000378:metabolism*; D010974:Platelet Aggregation / Q000187:drug effects; D012475:Salmonella; D012772:Shock, Septic / Q000209:etiology
D003470:Culture Media; D004731:Endotoxins; D010972:Platelet Activating Factor