Pituitary control of estrogen secretion in the chick embryo: true or spurious?
The aim of the present study was to examine whether the hypophysis played a role in ovarian 17 beta-estradiol secretion in the chick embryo. Hypophysectomy was fulfilled by partial decapitation, which has long been codified as a practical method of hypophysectomy in the chick embryo. Ovaries from decapitated, sham-operated, and intact embryos 9-19 days old were cultured in vitro during a 4-hr period, and 17 beta-estradiol released was determined by radioimmunoassay. A difference in estradiol secretion rate between decapitated and control embryos was noticed at 10-13 days, on both a per ovary and a per gram basis. At 15-19 days, there was no difference. These results suggest that the hypophysis influences the estrogen secretion of the chick embryo ovary during a limited space of time.
Weniger
J P
JP
Laboratoire de Zoologie et d'Embryologie expérimentale de l'Universitè Louis-Pasteur, Strasbourg, France.
Chouraqui
J
J
Zeis
A
A
D000704:Analysis of Variance; D000818:Animals; D002642:Chick Embryo; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D007016:Hypophysectomy; D010053:Ovary / Q000378:metabolism*; D010902:Pituitary Gland / Q000502:physiology*; D011863:Radioimmunoassay
D004958:Estradiol