Exclusion of an exon in monocyte alpha-1-antitrypsin mRNA after stimulation of U937 cells by interleukin-6.
Monocytes potentially express two mRNA species by alternative splicing of exons at the 5' end of the gene. During inflammation the plasma concentration of AAT can increase about three-fold. In a myelomonocytic cell line (U937), under basal conditions, two AAT mRNA species are produced. After stimulation by interleukin-6 (IL-6) one mRNA species is produced resulting from the exclusion of the second monocyte exon. These findings demonstrate a novel way in which the expression of mRNA can be modified in a single cell type.
Kalsheker
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N
Department of Medical Biochemistry, University of Wales College of Medicine, Cardiff Royal Infirmary, United Kingdom.
Swanson
T
T
D001483:Base Sequence; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005091:Exons; D015870:Gene Expression; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D012326:RNA Splicing; D012333:RNA, Messenger / Q000378:metabolism*; D000515:alpha 1-Antitrypsin / Q000235:genetics*
D015850:Interleukin-6; D012333:RNA, Messenger; D000515:alpha 1-Antitrypsin