Differential cellular localization of enzymes of L-arginine metabolism in the rat brain.
Polyclonal rabbit antisera specific to argininosuccinate synthetase (ASS), argininosuccinate lyase and arginase revealed that these enzymes of L-arginine metabolism are generally localized in different cells of the rat brain. In the main olfactory bulb and the cerebellar cortex the three immunoreactivities were localized in different cells: in the somatic motor nuclei ASS-like immunoreactivity was localized in incoming fibers, and the other two enzymes were found in the motor neurons. The results suggest that L-argininosuccinate and/or L-arginine may be transcellularly transported in the nervous system.
Nakamura
H
H
Department of Anatomy, Faculty of Medicine, Kagoshima University, Japan.
Saheki
T
T
Nakagawa
S
S
D000818:Animals; D001119:Arginase / Q000032:analysis*; D001123:Argininosuccinate Lyase / Q000032:analysis*; D001124:Argininosuccinate Synthase / Q000032:analysis*; D001921:Brain / Q000166:cytology / Q000201:enzymology*; D002525:Cerebellar Cortex / Q000201:enzymology; D007124:Immunoenzyme Techniques; D008297:Male; D009046:Motor Neurons / Q000201:enzymology; D009830:Olfactory Bulb / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains
D001119:Arginase; D001123:Argininosuccinate Lyase; D001124:Argininosuccinate Synthase