The effect of external gamma-irradiation on 59Fe release in vitro from alveolar macrophages previously having ingested 59Fe-iron hydroxide colloid.
The effect of external gamma-irradiation on the digestive function of rat alveolar macrophages (AM) was studied by using the in vitro assay system, where the 59Fe release from AM engulfing 59Fe-iron hydroxide colloid was measured as an indicator of their digestive capability. Graded doses of gamma-irradiation up to 100 Gy had no effect on the extracellular release of 59Fe in AM cultured at 4 and 37 degrees C for 8-72 hrs postirradiation. When 10 mM Ca-DTPA was added to the culture medium, the 59Fe release was slightly depressed by irradiation at a dose of 30 Gy. It is apparent from these results that gamma-irradiation had no effect on the 59Fe transfer between cell and medium, and little effect on the catabolism of 59Fe-colloid in rat AM.
Takahashi
S
S
National Institute of Radiological Sciences, Chiba, Japan.
Kubota
Y
Y
Sato
H
H
D000818:Animals; D002478:Cells, Cultured; D003102:Colloids; D005110:Extracellular Space / Q000378:metabolism*; D005290:Ferric Compounds / Q000008:administration & dosage*; D005720:Gamma Rays; D007504:Iron Radioisotopes / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism / Q000528:radiation effects*; D008297:Male; D011650:Pulmonary Alveoli / Q000166:cytology / Q000528:radiation effects; D051381:Rats; D011919:Rats, Inbred Strains
D003102:Colloids; D005290:Ferric Compounds; D007504:Iron Radioisotopes; C021024:ferric hydroxide