Arylamine N-acetyltransferase activities in cell lines of mouse, rat, hamster and man differing in their sensitivity to 1,6-dinitropyrene.
This study was aimed at monitoring N-acetyltransferase activities of continuous cell lines, which differ in their sensitivity to the toxic effects of nitroaromatic compounds. Transferase activities were measured toward the acetyl acceptors sulfamethazine and p-aminobenzoic acid in partially purified preparation of cytosols. Cell lines such as hamster V79, BHK, rat hepatoma H4IIEC3G- or fibroblast 208F, which are sensitive to 1,6-dinitropyrene (1,6-DNP), possess high transferase activities ranging from 120-270 nmol/min x mg protein. In contrast, human lung cells NCI-H322, mouse and rat hepatoma cells BW1J and H5, respectively, which are resistant to 1,6-DNP contain no or low transferase activity of less than 15 nmol/min x mg. There was no apparent correlation between 1,6-DNP sensitivity and acetyltransferase levels in a few cell lines, e.g. rat hepatoma HTC, 2sFou and 5L, which express intermediate transferase activities ranging from 25-50 nmol/min x mg protein. The results suggest that acetylation is an essential step in activating 1,6-DNP to toxic products in mammalian cells.
Srivastava
A K
AK
GSF-Institute of Toxicology, Gesellschaft für Strahlen- und Umweltforschung, Neuherberg, F.R.G.
Wiebel
F J
FJ
D000818:Animals; D001191:Arylamine N-Acetyltransferase / Q000378:metabolism*; D002460:Cell Line / Q000187:drug effects; D002478:Cells, Cultured; D006224:Cricetinae; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000201:enzymology; D051379:Mice; D011721:Pyrenes / Q000633:toxicity*; D051381:Rats; D013045:Species Specificity
D011721:Pyrenes; C033309:1,6-dinitropyrene; D001191:Arylamine N-Acetyltransferase