Repetitive sequences in the rat genome that have cleavage sites for the restriction endonuclease Taq I.
A cleavage of rat DNA with restriction endonuclease Taq I produced at least 8 discrete bands. One of the bands, 0.29 kilobase pairs (kb) long, was isolated and cloned in plasmid. The hybridization of this fragment to genomic DNA digested with Taq I showed 1.6 kb, 1.2 kb and 0.29 kb band in a smear hybridization signal. A repetition of the DNA sequence of this fragment was about 12,500 copies/haploid genome of both Buffalo and Sprague-Dawley rats by the Cot-hybridization analysis. Four independent clones of 0.29 kb Taq I fragment share high homology (97%).
Kikuchi
H
H
Research Institute for Tuberculosis and Cancer, Tohoku University, Sendai, Japan.
Sekiya
T
T
Nishimura
S
S
Watanabe
M
M
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification; D008099:Liver / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009691:Nucleic Acid Denaturation; D009695:Nucleic Acid Renaturation; D051381:Rats; D011919:Rats, Inbred Strains; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D013696:Temperature
D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases