title: Further experimental evidence in support of the involvement of ovarian follicles in oocyte maturation of the Indian catfish, Mystus vittatus.
abstract: The role of the ovarian follicular layer in the oocyte maturation of the Indian catfish, Mystus vittatus, was investigated in vitro using cyanoketone and epostane (specific inhibitors of the 3 beta-hydroxysteroid dehydrogenase enzyme system). Folliculated oocytes incubated with luteinizing hormone (LH) at the concentration of 10 micrograms/ml induced 67.7 +/- 1.5% of germinal vesicle breakdown (GVBD). When the oocytes were incubated with LH + cyanoketone or epostane, there was a significant drop in the frequency of GVBD. It revealed that the maturational response of M. vittatus oocytes to LH depends on the synthesis of delta 4 steroids (probably 17 alpha,20 beta-dihydroxy-4-pregen-3-one; 17 alpha,20 beta-diOHprog) from the follicular layer since both the inhibitors failed to abolish the maturational effect of 17 alpha, 20 beta-diOHprog (the most potent maturation-inducing steroid for this species).
authors: Centre of Advanced Study in Zoology, Banaras Hindu University, Varanasi-221 005, India.
authors: S
authors: S
authors: Haider
mesh_terms: D000737:Androstenols / Q000494:pharmacology; D000818:Animals; D002397:Catfishes / Q000502:physiology*; D003489:Cyanoketone / Q000494:pharmacology; D005260:Female; D006908:Hydroxyprogesterones / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D009866:Oogenesis / Q000187:drug effects / Q000502:physiology*; D006080:Ovarian Follicle / Q000502:physiology*
chemical_list: D000737:Androstenols; D006908:Hydroxyprogesterones; C007433:17,20-dihydroxy-4-pregnen-3-one; D003489:Cyanoketone; C046855:epostane