title: [The effective method of gene transfer into mammalian cells cultured in vitro].
abstract: Efficiency of transformation by a number of vectors with the different selective markers of a set of cell lines has been studied for three different methods based on using calcium phosphate, polybrene, or electroporation. Electroporation is shown to be the most efficient one. Using this method with the system rat2k-cells-pAGO vector we have obtained the frequencies of transformation up to 2-3.10(-3). We suggest to use this system as a model for investigation of homologous recombination in the framework of the gene therapy project.
authors: N I
authors: NI
authors: Vorontsova
authors: O V
authors: OV
authors: Evgrafov
authors: V B
authors: VB
authors: Makarov
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D015316:Genetic Therapy; D005822:Genetic Vectors; D006583:Hexadimethrine Bromide; D008322:Mammals; D014170:Transformation, Genetic
chemical_list: D006583:Hexadimethrine Bromide
vernacular_title: Effektivnyĭ metod perenosa genov v kletki mlekopitaiushchikh, kul'tiviruemye in vitro.