Dual promoters and tissue-specific expression of rat transthyretin gene.
The rat chromosomal gene for transthyretin was cloned and its dual promoter region was analyzed by primer extension. Semi-quantitative studies by primer extension analysis to determine the transcriptional start sites and their usage in the three tissues have suggested that the rat transthyetin gene has dual promoters: a major proximal promoter which is used in the liver and in the brain but not at all in the kidney, and a minor distal promoter which is totally used in the kidney and significantly in the brain.
Motojima
K
K
Department of Biochemistry, School of Pharmaceutical Sciences, Toho University, Chiba, Japan.
Goto
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D015870:Gene Expression; D005796:Genes; D015698:Genomic Library; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D009928:Organ Specificity; D011228:Prealbumin / Q000235:genetics*; D011401:Promoter Regions, Genetic; D051381:Rats; D015183:Restriction Mapping; D016385:TATA Box; D014158:Transcription, Genetic
D015345:Oligonucleotide Probes; D011228:Prealbumin