Characterization of the cDNA encoding proopiomelanocortin in the frog Rana ridibunda.
In the amphibian pars intermedia, secretion of proopiomelanocortin (POMC)-derived peptides is controlled by multiple factors including classical neurotransmitters and neuropeptides. To pursue questions concerning the regulation of POMC gene expression in Rana ridibunda, we have isolated and characterized a full-length cDNA for frog POMC. A cDNA clone isolated from a frog pituitary library contains an open-reading frame of 780-bp that predicts a 260 amino acid POMC protein. The structure of frog POMC demonstrates considerable amino acid sequence similarity with POMC from other species. In particular, the sequence of alpha-melanotropin (alpha-MSH) is identical in frog and all mammalian species studied so far, while adrenocorticotropin (ACTH) and beta-endorphin exhibit 79% and 84% homology with their human counterpart. Frog POMC contains only one potential asparagine-linked N-glycosylation signal (Asn-Ser-Thr) within the gamma-MSH domain. The alpha-MSH sequence is C-terminally flanked by the Gly-Lys-Lys amidation signal while the joining peptide is not amidate.
Hilario
E
E
Groupe de Recherche en Endocrinologie Moléculaire, URA CNRS 650, Unité Affiliée à l'INSERM, Université de Rouen, Mont-Saint-Aignan, France.
Lihrmann
I
I
Vaudry
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004247:DNA / Q000737:chemistry*; D005786:Gene Expression Regulation; D006031:Glycosylation; D006801:Humans; D008099:Liver / Q000737:chemistry; D008969:Molecular Sequence Data; D010902:Pituitary Gland / Q000737:chemistry; D011333:Pro-Opiomelanocortin / Q000096:biosynthesis / Q000235:genetics*; D011499:Protein Processing, Post-Translational; D011895:Rana ridibunda / Q000235:genetics*; D051381:Rats; D012474:Salmon; D012689:Sequence Homology, Nucleic Acid; D014982:Xenopus laevis
D011333:Pro-Opiomelanocortin; D004247:DNA