Enhancement of the mutagenicity of Trp-P-1, Trp-P-2 and benzo[a]pyrene by bupleuri radix extract.
Boiling-water extract of Korean-Saiko (Bupleuri Radix, from South Korea, Bupleurum falcatum L.) enhanced the mutagenic activity of Trp-P-1, Trp-P-2 and benzo[a]pyrene with S9mix. The boiling-water extract was fractionated with ether and then n-BuOH. Both the ether and the n-BuOH fractions also enhanced mutagenicity of Trp-P-1, respectively. The n-BuOH fraction was separated into seven fractions by silica gel chromatography and the chloroform eluate had the strongest enhancing effect on the mutagenic activity of Trp-P-1 with S9mix. The chloroform eluate fraction was further separated into five spots by thin-layer chromatography. Two of the spots had the strongest enhancing effect on the mutagenic activity of Trp-P-1. Since saikosaponin is a well known component in Bupleuri Radix, the effects of its existence were tested and saikosaponins a and c were found. The enhancement activity of saikosaponin a was very weak. The effective components are now being studied.
Niikawa
M
M
Ichimaru Pharcos Co., Ltd., Gifu, Japan.
Sakai
Y
Y
Ose
Y
Y
Sato
T
T
Nagase
H
H
Kito
H
H
Sato
M
M
Mizuno
M
M
D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D002243:Carbolines / Q000633:toxicity*; D004357:Drug Synergism; D066298:In Vitro Techniques; D009152:Mutagenicity Tests; D009153:Mutagens; D010936:Plant Extracts / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains
D002243:Carbolines; D009153:Mutagens; D010936:Plant Extracts; D001564:Benzo(a)pyrene; C019133:3-amino-1-methyl-5H-pyrido(4,3-b)indole; C019132:3-amino-1,4-dimethyl-5H-pyrido(4,3-b)indole