Nitrilase-catalyzed production of pyrazinoic acid, an antimycobacterial agent, from cyanopyrazine by resting cells of Rhodococcus rhodochrous J1.
Using resting cells of Rhodococcus rhodochrous J1, in which a large amount of nitrilase is induced, a simple and efficient bioconversion process for the production of pyrazinoic acid, an antimycobacterial agent, through catalysis by a nitrilase was developed. The reaction conditions for production of pyrazinoic acid were optimized. Under optimum conditions, 3.5 M cyanopyrazine was converted to pyrazinoic acid, with a molar conversion yield of 100%. The highest yield achieved corresponded to 434 g of pyrazinoic acid per liter of reaction mixture. The synthesized pyrazinoic acid was isolated and identified physico-chemically.
Kobayashi
M
M
Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University, Japan.
Yanaka
N
N
Nagasawa
T
T
Yamada
H
H
D000619:Aminohydrolases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D011718:Pyrazinamide / Q000031:analogs & derivatives* / Q000378:metabolism; D011719:Pyrazines / Q000378:metabolism*; D012240:Rhodococcus / Q000201:enzymology / Q000378:metabolism*; D013055:Spectrophotometry, Infrared; D013696:Temperature
D011719:Pyrazines; C066548:cyanopyrazine; D011718:Pyrazinamide; C005296:pyrazinoic acid; D000619:Aminohydrolases; C021867:nitrilase