Delineation of antigen contact residues on an MHC class II molecule.
This report describes a detailed mutational analysis of a major histocompatibility complex class II molecule--the alpha chain of the Ak complex. Each residue from 50-79 was replaced by an alanine, and the effects on recognition of Ak by panels of antibodies and T cells determined. The results provide the strongest existing experimental evidence that the antigen binding site on a class II molecule can be modelled on the crystal structure of a class I molecule. The data have also permitted the delineation of residues that actually contact antigenic peptides.
Peccoud
J
J
Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Faculté de Médecine, Strasbourg, France.
Dellabona
P
P
Allen
P
P
Benoist
C
C
Mathis
D
D
D000409:Alanine / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001667:Binding, Competitive; D000949:Histocompatibility Antigens Class II / Q000737:chemistry* / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009113:Muramidase / Q000235:genetics* / Q000276:immunology; D016297:Mutagenesis, Site-Directed; D010446:Peptide Fragments / Q000235:genetics* / Q000276:immunology; D011487:Protein Conformation; D015203:Reproducibility of Results; D012260:Ribonucleases / Q000235:genetics* / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014961:X-Ray Diffraction
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; D010446:Peptide Fragments; C066760:bovine ribonuclease peptide (41-61); C066759:hen egg lysozyme peptide (46-61); D012260:Ribonucleases; D009113:Muramidase; D000409:Alanine