Antibody-coated liposomes as a particulate solid phase for immunoassays. Measurement of urinary 'micro-albumin'.
A novel use of liposomes as a solid phase material achieving separation in immunoassays is described. Antibody-coated liposomes were prepared and used as a particulate solid phase in a radioimmunoassay procedure for urinary albumin. The assay was compared to a liquid phase albumin radioimmunoassay. The potential benefits of liposomes over other particulate solid phases are discussed. The use of liposomes in this manner need not be restricted to radioimmunoassay but should also be applicable to other immunoassays using alternative non-isotopic labels.
Frost
S J
SJ
Clinical Biochemistry Department, Hurstwood Park Neurological Centre, Haywards Heath, West Sussex, U.K.
Firth
G B
GB
Chakraborty
J
J
D000419:Albuminuria / Q000652:urine*; D000906:Antibodies; D002498:Centrifugation; D003920:Diabetes Mellitus / Q000652:urine; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008081:Liposomes; D010714:Phosphatidylethanolamines; D011233:Precipitin Tests; D013696:Temperature; D013997:Time Factors
D000906:Antibodies; D008081:Liposomes; D010714:Phosphatidylethanolamines