title: Rhizobium leguminosarum exoB mutants are deficient in the synthesis of UDP-glucose 4'-epimerase.
abstract: Rhizobium leguminosarum bv. viciae Exo- mutant strains RBL5523,exo7::Tn5,RBL5523,exo8::Tn5 and RBL5523,exo52::Tn5 are affected in nodulation and in the syntheses of lipopolysaccharide, capsular polysaccharide, and exocellular polysaccharide. These mutants were complemented for nodulation and for the syntheses of these polysaccharides by plasmid pMP2603. The gene in which these mutants are defective is functionally homologous to the exoB gene of Rhizobium meliloti. The repeating unit of the residual amounts of EPS still made by the exoB mutants of R. leguminosarum bv. viciae lacks galactose and the substituents attached to it. The R. leguminosarum bv. viciae and R. meliloti exoB mutants fail to synthesize active UDP-glucose 4'-epimerase.
authors: Department of Plant Molecular Biology, Leiden University, The Netherlands.
authors: H C
authors: HC
authors: Canter Cremers
authors: M
authors: M
authors: Batley
authors: J W
authors: JW
authors: Redmond
authors: L
authors: L
authors: Eydems
authors: M W
authors: MW
authors: Breedveld
authors: L P
authors: LP
authors: Zevehuizen
authors: E
authors: E
authors: Pees
authors: C A
authors: CA
authors: Wijffelman
authors: B J
authors: BJ
authors: Lugtenberg
mesh_terms: D002240:Carbohydrate Sequence; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D005838:Genotype; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009154:Mutation; D009844:Oligosaccharides / Q000096:biosynthesis; D010957:Plasmids; D012231:Rhizobium / Q000201:enzymology / Q000235:genetics*; D013559:Symbiosis; D014534:UDPglucose 4-Epimerase / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D009844:Oligosaccharides; D014534:UDPglucose 4-Epimerase