Electrophoresis of DNA fragments in gels from acrylamide-rich copolymers.
Two polyacrylamide-rich, non-toxic, gelable copolymers have been developed to facilitate the formation of user-cast electrophoresis gels. Gel formation is accomplished with dithiothreitol as the chemical cross-linking agent. The higher molecular weight copolymer is suitable for casting gels of copolymer concentration less than or equal to 8%. Gels of 3% concentration are excellent for resolving dsDNA fragments up to approximately 3000 base pairs. Because the cross-linking chemistry is not thwarted by the presence of urea, it is also possible to cast denaturing gels with these copolymers.
Christy
K G
KG
Life Sciences Research, Eastman Kodak Company, Rochester, NY 14650-2122.
LaTart
D B
DB
Osterhoudt
H W
HW
Ponticello
I S
IS
D000180:Acrylic Resins; D003432:Cross-Linking Reagents; D004247:DNA / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D046911:Macromolecular Substances; D008970:Molecular Weight; D000099166:Polyacrylamides
D000180:Acrylic Resins; D003432:Cross-Linking Reagents; D004247:DNA; D046911:Macromolecular Substances; D000099166:Polyacrylamides