Analysis of a gene cluster for sarcotoxin II, a group of antibacterial proteins of Sarcophaga peregrina.
Sarcotoxin II is a group of antibacterial proteins of Sarcophaga peregrina (flesh fly) with related primary structures. We have cloned three genes in this family. These genes formed a tandem array with about 2-kb intervals, and one of them was present in the opposite strand. The putative amino acid sequences of the proteins encoded by these genes were very similar except for a deletion in one of them. All of the genes were found to be activated transiently in the same way when the larval body wall was injured, suggesting that the encoded proteins are acute-phase-responsive proteins for protecting the insect from bacterial infection.
Kanai
A
A
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Natori
S
S
D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004175:Diptera / Q000235:genetics*; D007301:Insect Hormones / Q000235:genetics*; D019476:Insect Proteins; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D005810:Multigene Family; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D000890:Anti-Infective Agents; D007301:Insect Hormones; D019476:Insect Proteins; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; C052148:sarcotoxin IIA protein, Sarcophaga peregrina; C052149:sarcotoxin IIB protein, Sarcophaga peregrina; C052150:sarcotoxin IIC protein, Sacrophaga peregrina; D004247:DNA