Similarities between neuronal Lewy bodies in parkinsonism and hepatic Mallory bodies in alcoholism.
The aim of the present study was to identify components of the Lewy body, which is a characteristic neuronal lesion in idiopathic Parkinsonism, using histochemical methods that selectively stain the Mallory body, a characteristic lesion of the hepatocyte in alcoholism. Our observation that Lewy bodies stain with phosphotungstic acid hematoxylin, the dye originally used for demonstrating alcoholic hyaline (Mallory bodies), promoted this study. The material consisted of formalin-fixed, brain stem tissue from Parkinsonian subjects, and of similarly preserved liver tissues from alcoholic individuals. The methods selected were Roque's chromotrope 2R-aniline blue, and Liisberg's rhodamine B, which stains Mallory bodies due to its affinity for sites of tissue keratinization. Hence, skin was also included in this study as control tissue. Our results showed that Lewy bodies in the brain, Mallory bodies in the liver and stratum corneum in the skin have identical staining properties with the dyes used, indicating the presence of histochemically similar components. Taking into account the reactions of these dyes with model substances, we suggest that the similar components shared by Lewy bodies and Mallory bodies are arginine-rich proteins and lipids associated with keratinization. Similar findings in both, a toxin-induced lesion of the liver, and a spontaneous lesion of the brain may offer clues for understanding the latter's mode of formation.
Issidorides
M R
MR
Department of Psychiatry, University of Athens Medical School, Eginition Hospital, Greece.
Panayotacopoulou
M T
MT
Tiniacos
G
G
D000437:Alcoholism / Q000473:pathology*; D001921:Brain / Q000473:pathology*; D006651:Histocytochemistry; D006801:Humans; D008099:Liver / Q000648:ultrastructure*; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000648:ultrastructure*; D010300:Parkinson Disease / Q000473:pathology*