Effect of dietary cholesterol on the lipoprotein profile and binding of radioiodinated lipoproteins to hepatic membranes in the cockerel (Gallus domesticus).
1. Cockerels fed a cholesterol-supplemented diet experienced a marked elevation of lipoprotein particles of density less than or equal to 1.006 g/ml (VLDL) and a diminution of lipoprotein particles of density 1.02-1.05 g/ml (LDL). 2. Unlike VLDL of some cholesterol-fed animals, cholesterol-fed cockerel VLDL did not display beta-mobility on agarose gel electrophoresis. 3. [125I]LDL and [125I]HDL binding to cockerel liver membranes was not affected by cholesterol feeding. 4. Different lipoprotein types appear to bind to a common site on cockerel liver membranes. 5. The results suggest that liver cells of cockerels may not possess LDL binding sites that are analogous to those of mammalian species.
Loo
G
G
Department of Physiology and Biophysics, Howard University College of Medicine, Washington, DC 20059.
Wong
H Y
HY
Kliman
P G
PG
Berlin
E
E
Peters
R
R
Sherief
H T
HT
Zhuang
H
H
Allen
P C
PC
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002645:Chickens; D002791:Cholesterol, Dietary / Q000494:pharmacology*; D004587:Electrophoresis, Agar Gel; D007457:Iodine Radioisotopes; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008079:Lipoproteins, VLDL / Q000378:metabolism*; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male
D002791:Cholesterol, Dietary; D007457:Iodine Radioisotopes; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL