A new form of baker's yeast transketolase. An enzyme-RNA complex.
Using an immunosorbent, a new form of transketolase, namely, an enzyme-RNA complex, was isolated from a baker's yeast extract. Spontaneous fission of RNA (or its enzymic hydrolysis by RNase) is accompanied by a sharp increase in the catalytic activity of transketolase, which may be directly related to the enzyme's regulation mechanism.
Tikhomirova
N K
NK
A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.
Merchan
A Y
AY
Kochetov
G A
GA
D002850:Chromatography, Gel; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D012331:RNA, Fungal / Q000302:isolation & purification* / Q000378:metabolism; D012259:Ribonuclease, Pancreatic / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013056:Spectrophotometry, Ultraviolet; D014174:Transketolase / Q000302:isolation & purification* / Q000378:metabolism
D012331:RNA, Fungal; D014174:Transketolase; D012259:Ribonuclease, Pancreatic