Characterization of 5-HT3 receptors in intact N1E-115 neuroblastoma cells.
The highly selective 5-HT3 receptor antagonist [3H]GR65630 has been used to characterize 5-HT3 receptors in intact N1E-115 neuroblastoma cells. Equilibrium binding analysis demonstrated high-affinity binding to a single class of receptors with a Kd of 0.69 (+/- 0.12) nM and Bmax of 31.4 (+/- 11.4) fmol/10(5) cells, equivalent to approximately 200,000 sites per cell. Specific binding was displaced by low concentrations of 5-HT3-selective ligands, and by the nicotinic antagonist d-tubocurarine.
Lummis
S C
SC
Molecular Neurobiology Unit, MRC Centre, Cambridge, U.K.
Kilpatrick
G J
GJ
Martin
I L
IL
D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D007093:Imidazoles; D007211:Indoles; D007700:Kinetics; D008024:Ligands; D051379:Mice; D009447:Neuroblastoma / Q000378:metabolism*; D011985:Receptors, Serotonin / Q000737:chemistry / Q000378:metabolism*; D012702:Serotonin Antagonists / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000378:metabolism
D007093:Imidazoles; D007211:Indoles; D008024:Ligands; D011985:Receptors, Serotonin; D012702:Serotonin Antagonists; C059068:GR 65630