Distribution of IL-3 activity in mice transplanted with IL-3 producing T cells (STIL-3): abrogation of the distribution of STIL-3 cells into the liver and liver hemopoiesis by splenectomy.
Distribution of IL-3 producing T cells, STIL-3, in splenectomized mice after intravenous injection was studied in order to determine the site of IL-3 production in the splenectomized mice. Hematological features of STIL-3-inoculated mice after splenectomy were also studied. Splenectomy prior to inoculation of STIL-3 cells resulted in a slight increase in the level of IL-3 activity in most lymphohemopoietic tissues, but resulted in a slight decrease in the level of IL-3 activity in the plasma. The splenectomy also abrogated granulocytosis which was evident in sham-operated host mice. Hepatomegaly and a number of granulocytic foci in the liver were observed in STIL-3 inoculated mice, but this was again abrogated completely by the splenectomy. These results suggest that the spleen is the major site of granulopoiesis in response to the stimulus with IL-3, and also that the spleen is essential for the migration or metastasis of STIL-3 cells to the liver.
Aramaki
K
K
Department of Biology, Faculty of Science, Niigata University, Japan.
Takayama
N
N
Taniguchi
S
S
Miyashita
M
M
Sugimoto
K
K
Mori
K J
KJ
D000818:Animals; D002452:Cell Count; D005260:Female; D006098:Granulocytes / Q000166:cytology; D006410:Hematopoiesis; D007377:Interleukin-3 / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D011184:Postoperative Period; D013156:Splenectomy; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism / Q000637:transplantation*
D007377:Interleukin-3