title: Biochemical characterization of Plasmodium falciparum hemozoin.
abstract: Hemozoin, the pigment granule which develops within the blood stage food vacuole of the malaria parasite Plasmodium falciparum, was biochemically characterized. Hemozoin was found to be composed of 65% protein, 16% ferriprotoporphyrin-IX (hematin), 6% carbohydrate, and trace amounts of lipid and nucleic acids. The overwhelming majority of the protein component is a mixture of native and denatured human globin non-covalently associated with the metalloporphyrin. Immunoelectron microscopy, employing anti-human hemoglobin as a probe, identified in situ association of hemoglobin with hemozoin. Hemozoin produced within diabetic blood had a higher proportion of carbohydrate, suggesting that the carbohydrate component comes from non-enzymatic glycosylation of hemoglobin.
authors: New York University Medical Center, New York.
authors: P
authors: P
authors: Goldie
authors: E F
authors: EF
authors: Roth
authors: J
authors: J
authors: Oppenheim
authors: J P
authors: JP
authors: Vanderberg
mesh_terms: D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000737:chemistry / Q000469:parasitology / Q000648:ultrastructure; D006420:Hemeproteins / Q000737:chemistry* / Q000302:isolation & purification; D006427:Hemin / Q000032:analysis; D006454:Hemoglobins / Q000032:analysis; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D010860:Pigments, Biological / Q000737:chemistry* / Q000302:isolation & purification; D010963:Plasmodium falciparum / Q000032:analysis* / Q000648:ultrastructure; D015800:Protozoan Proteins / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D002241:Carbohydrates; D006420:Hemeproteins; D006454:Hemoglobins; D010860:Pigments, Biological; D015800:Protozoan Proteins; C020753:hemozoin; D006427:Hemin