A method for performing full-thickness, orthotopic, penetrating keratoplasty in the mouse.
In an attempt to overcome the lack of well characterized, inbred, and congenic strains, as well as a paucity of immunological reagents, in rabbit and rat models, we have developed a procedure for performing full-thickness, penetrating keratoplasty in the mouse. One hundred percent of isografts (BALB/c onto BALB/c) were successful, while 83.3% of allografts (C3H onto BALB/c) failed. The use of a mouse model to study corneal graft failure will allow a critical evaluation of the role of a variety of immunological mediators in causing graft failure.
She
S C
SC
Department of Ophthalmology, Southern Illinois University, School of Medicine, Springfield 62794-9230.
Steahly
L P
LP
Moticka
E J
EJ
D000818:Animals; D003315:Cornea / Q000098:blood supply / Q000473:pathology; D003318:Corneal Opacity / Q000209:etiology / Q000473:pathology; D005260:Female; D006084:Graft Rejection; D015948:Keratoplasty, Penetrating / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D011183:Postoperative Complications; D014184:Transplantation, Homologous; D014185:Transplantation, Isogeneic