Calcium protects pituitary basic fibroblast growth factors from limited proteolysis by co-purifying proteases.
Extracts from bovine pituitaries and other tissues contained basic fibroblast growth factor-like peptides of 22-26 kda, co-fractionating with smaller, 16-20 kda bFGFs. Heparin-bound, 22-26 kda bFGFs were converted to smaller, heparin-binding forms by tryptic proteolysis. In solution, 22-26 kda bFGFs were converted to smaller, heparin-binding forms by an activity present in pituitary extracts. Calcium protected higher molecular weight pituitary bFGFs from truncation by the endogenous activity, which was not acid-activated, co-purified with bFGF during heparin-sepharose chromatography, remained operant at high salt concentrations and was inhibited by phenylmethan-sulfonyl fluoride.
Doble
B W
BW
Division of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, Winnipeg, Canada.
Fandrich
R R
RR
Liu
L
L
Padua
R R
RR
Kardami
E
E
D000818:Animals; D015153:Blotting, Western; D002118:Calcium / Q000494:pharmacology*; D002846:Chromatography, Affinity; D016222:Fibroblast Growth Factor 2 / Q000302:isolation & purification*; D006493:Heparin; D008970:Molecular Weight; D010902:Pituitary Gland / Q000737:chemistry* / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D014357:Trypsin / Q000494:pharmacology*
D016222:Fibroblast Growth Factor 2; D006493:Heparin; D014357:Trypsin; D002118:Calcium