Fate and uptake of soluble and particulate antigens in the preparturient bovine mammary gland.
Pregnant heifers were infused intramammarily with ovalbumin in one quarter and killed S. uberis in another quarter. The animals were slaughtered at time intervals after infusion, and tissues were collected from different parts of the gland and from the local lymph nodes. Ovalbumin and bacteria reached the lymph nodes as early as 1 and 3 h after infusion respectively. Ovalbumin was also present in blood 5-10 min after infusion and in non-infused quarters. Both ovalbumin and bacteria were phagocytosed by the epithelium of the mammary gland. However, bacteria were seen in the tissues in small numbers at all stages after infusion. Larger numbers were phagocytosed by neutrophils in the lumen of ducts, 18 h after infusion. Phagocytosis of both ovalbumin and bacteria was not present in the connective tissue of the gland. The results indicate increased permeability of the preparturient gland to soluble proteins, but limited uptake of bacteria. Furthermore, a mechanism of transfer of antigens among quarters has been suggested.
Nashar
T O
TO
Department of Veterinary Medicine, University of Bristol, Langford, Great Britain.
Williams
M R
MR
Brown
P J
PJ
Cripps
P J
PJ
Stokes
C R
CR
D000818:Animals; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000493:pharmacokinetics*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D004848:Epithelium / Q000378:metabolism; D005260:Female; D007124:Immunoenzyme Techniques; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000276:immunology / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism; D010047:Ovalbumin / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D010539:Permeability; D010587:Phagocytosis / Q000276:immunology; D011247:Pregnancy; D013291:Streptococcus / Q000276:immunology
D000942:Antigens, Bacterial; D010047:Ovalbumin