The suprachiasmatic nucleus of the rat hypothalamus in culture: an anatomical and electrophysiological study.
This study shows that foetal neurons from the suprachiasmatic area, after dissociation and culture, contain in vitro the same characteristics as are found in the in vivo situation. The main peptidergic neurotransmitters present in the suprachiasmatic nucleus in vivo, vasoactive intestinal polypeptide (VIP) and vasopressin, are expressed in vitro while the cytoskeleton of these cells possesses phosphorylated neurofilaments. The exclusive uptake of Lucifer Yellow liposomes by neurons is also refound in suprachiasmatic cultures. The electrophysiological results are in agreement with those characteristics found in vitro and in vivo.
Walsh
I
I
Department of Physiology, University of Leiden, The Netherlands.
Marani
E
E
vd Berg
R J
RJ
Rietveld
W J
WJ
D000200:Action Potentials / Q000502:physiology; D000818:Animals; D046508:Culture Techniques; D004337:Drug Carriers; D005456:Fluorescent Dyes; D007150:Immunohistochemistry; D007382:Intermediate Filaments / Q000737:chemistry; D007546:Isoquinolines; D008081:Liposomes; D051381:Rats; D013493:Suprachiasmatic Nucleus / Q000033:anatomy & histology* / Q000196:embryology / Q000502:physiology*; D014660:Vasoactive Intestinal Peptide / Q000032:analysis; D014667:Vasopressins / Q000032:analysis
D004337:Drug Carriers; D005456:Fluorescent Dyes; D007546:Isoquinolines; D008081:Liposomes; D014667:Vasopressins; D014660:Vasoactive Intestinal Peptide; C017475:lucifer yellow