[Effect of retinoids on the growth of corneal endothelial cells in culture].
Corneal endothelium is a target tissue for retinoids and epidermal growth factor (EGF). We report here that retinoic acid and its synthetic analog, CBS-211 A (10(-8)-10(-7) M), enhance the mitogenic effect of EGF on cultured bovine corneal endothelial cells (BCEC). Furthermore, retinoid treatment increases the EGF-binding capacity of BCEC. The addition of cycloheximide (0.5 micrograms/ml) to cultures simultaneously with retinoids suppressed the retinoid effect on EGF-binding. These results suggest that retinoids could induce the expression of EGF receptors on BCEC as an early event. This study demonstrates a cooperation between retinoids and EGF in corneal endothelium and suggests that retinoids could have a beneficial effect on this tissue repair in vivo.
Bonne
C
C
Laboratoire de Physiologie Cellulaire, Université de Montpellier I.
Junquero
D
D
Driot
J Y
JY
Coquelet
C
C
Modat
G
G
D000818:Animals; D001565:Benzoates / Q000494:pharmacology*; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004728:Endothelium, Corneal / Q000166:cytology* / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000378:metabolism / Q000494:pharmacology; D066246:ErbB Receptors / Q000378:metabolism; D006801:Humans; D014212:Tretinoin / Q000494:pharmacology*
D001565:Benzoates; C062800:CBS 211 A; D014212:Tretinoin; D004815:Epidermal Growth Factor; D066246:ErbB Receptors
Action des rétinoïdes sur la croissance des cellules endothéliales cornéennes en culture.