[Electron microscopic observation of differentiation of leukemic cell clone after CFU-Mix culture].
By scanning and transmission electron microscopy, leukemic cells were observed after CFU-Mix culture. Even though granulocytic growth factor, erythropoietin and lymphocytic growth factor were added in vitro, acute leukemic cells still showed defective differentiation and maturation. These were characterized by abnormal colony which consisted of smooth cells, bizarre shape, nuclear-cytoplasmic asynchrony in development, and appearance of nuclear bleb. However, chronic myelogenous leukemic cells were more mature than the acute ones, manifesting in normal colony with finger-like projections and ruffled membrane. Macrophages and eosinophils could be observed. It is suggested that there is a difference in differentiation between acute and chronic leukemic cells.
Chen
Y
Y
Union Hospital, Tongji Medical College, Wuhan.
D000293:Adolescent; D000328:Adult; D005260:Female; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000473:pathology*; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000648:ultrastructure*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000648:ultrastructure