title: Amino acid sequence of a 12-kDa inhibitor of protein kinase C.
abstract: The complete primary structure of a bovine-brain-derived inhibitor of protein kinase C has been established. Fragments of the purified protein were obtained by cleavage with cyanogen bromide, Staphylococcus aureus V8 protease, trypsin and chymotrypsin. Subsequent analysis of the resulting fragments by fast-atom-bombardment mass spectrometry and Edman degradation revealed a calculated molecular mass of 11,779 Da with the following 107-amino-acid sequence: [sequence: see text] This inhibitor does not share significant primary structural identity with any other known protein.
authors: Biochemistry Department, Upjohn Laboratories, Kalamazoo, MI 49001.
authors: N M
authors: NM
authors: Mozier
authors: H A
authors: HA
authors: Zürcher-Neely
authors: D M
authors: DM
authors: Guido
authors: W R
authors: WR
authors: Mathews
authors: R L
authors: RL
authors: Heinrikson
authors: E D
authors: ED
authors: Fraser
authors: M P
authors: MP
authors: Walsh
authors: J D
authors: JD
authors: Pearson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000201:enzymology; D002417:Cattle; D002918:Chymotrypsin; D003488:Cyanogen Bromide; D004791:Enzyme Inhibitors / Q000737:chemistry*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D014357:Trypsin
chemical_list: D004791:Enzyme Inhibitors; D010446:Peptide Fragments; D011493:Protein Kinase C; D002918:Chymotrypsin; D014357:Trypsin; D003488:Cyanogen Bromide