Infarcted heart uptake and biodistribution of radiolabelled anti-myosin monoclonal antibody in rat and dog myocardial infarct models.
A new mouse monoclonal antibody that recognizes alpha- and beta-heavy chains of human atrial and ventricular myosin and beta-heavy chain of human slow skeletal muscle myosin was obtained. The 125I- and 111In-labelled antibody, and its F(ab')2 and Fab fragments localize in isoproterenol induced infarcted rat heart, with the F(ab')2 fragment showing the highest uptake. Comparison with 99Tc-pyrophosphate uptake in infarcted dog heart, induced by selective obstruction of a coronary artery, suggest that the 111In-labelled F(ab')2 localizes specifically in infarcted myocardium only.
Sikorska
H
H
Rougier Bio-Tech Ltd., Montreal, Quebec, Canada.
Rousseau
J
J
Desputeau
C
C
Gervais
A
A
Savoie
S
S
Ghaffari
M A
MA
Bisson
L
L
van Lier
J E
JE
D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics*; D004285:Dogs; D005260:Female; D007140:Immunoglobulin Fab Fragments / Q000493:pharmacokinetics; D007205:Indium Radioisotopes; D007457:Iodine Radioisotopes; D009203:Myocardial Infarction / Q000000981:diagnostic imaging / Q000378:metabolism*; D009942:Organometallic Compounds / Q000493:pharmacokinetics*; D011877:Radionuclide Imaging; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D007205:Indium Radioisotopes; D007457:Iodine Radioisotopes; D009942:Organometallic Compounds; C058899:imciromab pentetate