[Detection of polyamines by a new enzymatic differential assay. (8) Studies on tissue polyamine concentrations in patients with genitourinary malignant diseases].
Polyamine concentrations of human cancerous and non-cancerous tissues from the kidney, ureter, bladder were measured by a new enzymatic method for isolation and determination of polyamines. In cancerous and non-cancerous tissue of the organs studied, the spermine level was highest followed by the spermidine and diamine levels. The concentrations of diamine, spermidine and spermine in cancerous tissues were significantly higher than those in non-cancerous tissues, but there was no significant difference in the spermidine/spermine ratio between the cancerous and non-cancerous tissues. These data suggest that polyamines are produced above the normal levels in pathological conditions such as renal cell carcinoma, ureteral cancer and bladder cancer.
Koide
T
T
Department of Urology, Gifu Prefectural Gifu Hospital.
Sakai
S
S
Kawada
Y
Y
Hara
A
A
Sawada
H
H
D015317:Biogenic Polyamines / Q000032:analysis*; D001681:Biological Assay / Q000379:methods; D002292:Carcinoma, Renal Cell / Q000737:chemistry; D004273:DNA, Neoplasm / Q000032:analysis; D003959:Diamines / Q000032:analysis; D006801:Humans; D007668:Kidney / Q000737:chemistry; D007680:Kidney Neoplasms / Q000737:chemistry; D013095:Spermidine / Q000032:analysis; D013096:Spermine / Q000032:analysis; D014513:Ureter / Q000737:chemistry; D014516:Ureteral Neoplasms / Q000737:chemistry; D001743:Urinary Bladder / Q000737:chemistry; D001749:Urinary Bladder Neoplasms / Q000737:chemistry; D014571:Urologic Neoplasms / Q000737:chemistry*
D015317:Biogenic Polyamines; D004273:DNA, Neoplasm; D003959:Diamines; D013096:Spermine; D013095:Spermidine