[Contribution of lysosomal enzymes into degradation of short- and long-lived proteins in human embryonal fibroblasts with abnormal chromosome complement].
Degradation of short- and long-life proteins was studied in human embryonal fibroblasts with normal karyotype, trisomy and triploidy. Degradation of both short- and long-life proteins by means of lysosomal enzymes was elevated in fibroblasts with anomalous karyotype at the logarithmic phase of growth as compared with normal state, when lysosomotropic agent NH4Cl was used. In transition to stationary phase of growth participation of the lysosomal system in degradation of long-life proteins was increased in fibroblasts with normal karyotype, whereas degradation of these proteins in aneuploid fibroblasts maintained at the level found during the logarithmic phase of growth. Lysosomal enzymes did not participate apparently in degradation of short-life proteins in fibroblasts with normal and anomalous karyotype at both phases of growth. The data obtained suggest that chromosomal disbalance was related to impaired regulation of protein degradation as well as to possible alteration in the ratio of cytosol and lysosomal proteolysis.
Golubeva
N V
NV
Podobed
O V
OV
Kukharenko
V I
VI
Del'vig
A A
AA
D000643:Ammonium Chloride / Q000494:pharmacology; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D004622:Embryo, Mammalian; D010450:Endopeptidases / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology*; D011506:Proteins / Q000378:metabolism*; D013997:Time Factors
D011506:Proteins; D000643:Ammonium Chloride; D010450:Endopeptidases
Issledovanie vklada lizosomal'nykh fermentov v degradatsiiu korotko- i dolgozhivushchikh belkov émbrional'nykh fibroblastov cheloveka s anomal'nym naborom khromosom.