Expression of the avian gag-myc oncogene in Saccharomyces cerevisiae.
The gag-myc oncogenic sequence of the avian retrovirus MC29 was first inserted in a multicopy expression vector allowing its expression in Saccharomyces cerevisiae. The oncogene transcripts were detected in yeast by Northern blot hybridization and gag-myc proteins were revealed by immunoprecipitation. On solid medium, the average size of gag-myc transformant colonies was smaller than control. In liquid cultures, the gag-myc strains had a doubling time of 4.7 h compared with 3.1 h in the controls. In one of the recipient strains, and after an initial transient period of 5 days, the gag-myc transformants became physiologically indistinguishable from control. In another recipient strain, the slow-growth phenotype is permanent. Plasmid instability is increased in gag-myc transformants. When a single copy of the gag-myc gene was inserted in a yeast chromosome, no phenotype was observed, showing that slow growth is the consequence of plasmid loss.
Durrens
P
P
Laboratoire de Génétique, Unité Associée du Centre National de la Recherche Scientifique URA, Talence, France.
Fournier
A
A
Desfarges
L
L
Aigle
M
M
D000818:Animals; D001483:Base Sequence; D001717:Birds / Q000235:genetics*; D015152:Blotting, Northern; D015825:Chromosomes, Fungal / Q000378:metabolism; D003001:Cloning, Molecular; D015870:Gene Expression; D015750:Genes, gag; D016259:Genes, myc; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009857:Oncogenes; D010957:Plasmids; D011233:Precipitin Tests; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D014158:Transcription, Genetic; D014170:Transformation, Genetic