Liver phosphatidylcholine hydroperoxidation provoked by ethionine-containing choline-deficient diet in mice.
It is shown that peroxidation of phosphatidylcholine (PC) is enhanced in liver of mice fed a hepatocarcinogenic choline-deficient diet containing 0.1% w/w ethionine. Mice were divided into 4 groups and fed for 4 weeks one of the following diets: choline-supplemented; choline-supplemented containing ethionine; choline-deficient; and choline-deficient containing ethionine. Phosphatidylcholine hydroperoxide (PCOOH) of liver lipids was measured by high performance liquid chromatography using a chemiluminescence detector. Mice fed a choline-deficient diet containing ethionine showed 6-fold higher PCOOH levels than the choline-supplemented control mice: the PCOOH/PC molar ratios of liver lipids were 32.3 X 10(-5) and 5.6 X 10(-5), respectively. In addition to this remarkable degree of lipid peroxidation in liver of mice fed the choline-deficient diet containing ethionine, we also observed a significant liver fatty infiltration, a decrease in plasma and liver alpha-tocopherol, and an increase in liver injury-indicative enzyme activities. Also, marker enzymes for hepatocarcinogenesis, glucose-6-phosphatase and gamma-glutamyl transpeptidase were affected. These data suggest that enhanced hydroperoxidation of phosphatidylcholine may participate in hepatocarcinogenesis provoked by choline deficiency in the presence of ethionine.
Yoshida
L S
LS
Department of Food Chemistry, Tohoku University, Sendai, Japan.
Miyazawa
T
T
Fujimoto
K
K
Kaneda
T
T
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002796:Choline Deficiency / Q000097:blood / Q000378:metabolism*; D005001:Ethionine / Q000633:toxicity*; D005234:Fatty Liver / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009929:Organ Size / Q000187:drug effects; D010713:Phosphatidylcholines / Q000378:metabolism*
D010713:Phosphatidylcholines; C057590:phosphatidylcholine hydroperoxide; D005001:Ethionine