title: A novel neutrophil chemoattractant generated during an inflammatory reaction in the rabbit peritoneal cavity in vivo. Purification, partial amino acid sequence and structural relationship to interleukin 8.
abstract: An inflammatory reaction was induced in vivo by injection of zymosan into the peritoneal cavity of the rabbit. The inflammatory exudate was found to contain oedema-inducing and neutrophil chemoattractant activity when assayed in rabbit skin in vivo, using 125I-albumin and 111In-neutrophils. This activity was additional to that of complement fragment C5a, which was removed by an affinity gel. Two chemoattractants were isolated by cation-exchange, gel-filtration and reversed-phase h.p.l.c. One of these, which ran as a single band of 6-8 kDa on SDS/PAGE, was subjected to N-terminal sequence analysis without reduction and alkylation of cysteine residues. Positive identification of 28 of the first 31 amino acids revealed a rabbit homologue of interleukin-8 (75% sequence identity with human interleukin-8). The demonstration of interleukin-8 as a major neutrophil chemoattractant in an inflammatory reaction in vivo provides the basis for further investigations into the role of this cytokine in the inflammatory process.
authors: Department of Applied Pharmacology, National Heart and Lung Institute, London, U.K.
authors: B C
authors: BC
authors: Beaubien
authors: P D
authors: PD
authors: Collins
authors: P J
authors: PJ
authors: Jose
authors: N F
authors: NF
authors: Totty
authors: J
authors: J
authors: Hsuan
authors: M D
authors: MD
authors: Waterfield
authors: T J
authors: TJ
authors: Williams
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002630:Chemotactic Factors / Q000096:biosynthesis*; D004487:Edema / Q000097:blood / Q000139:chemically induced; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D007249:Inflammation / Q000097:blood* / Q000139:chemically induced; D016209:Interleukin-8 / Q000737:chemistry / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000378:metabolism*; D010538:Peritonitis / Q000097:blood* / Q000139:chemically induced; D011817:Rabbits; D012967:Sodium Dodecyl Sulfate; D015054:Zymosan
chemical_list: D002630:Chemotactic Factors; D016209:Interleukin-8; D012967:Sodium Dodecyl Sulfate; D015054:Zymosan