Flow cytometry as a tool to discriminate respiratory-competent and respiratory-deficient yeast cells.
The cationic lipophilic dye Rhodamine 123 (Rh123) is selectively enriched in mitochondria in a membrane potential-dependent manner. Application of drugs which interfere with the electron flow of the respiratory chain lead to a severe reduction of mitochondrial dye uptake. In this communication we show that the same effect is observed after Rh123-staining of respiratory-deficient yeast mutants. Based on this observation we used flow cytometry to discriminate respiratory-competent and respiratory-deficient yeast cells. Combined with a cell sorter we were able to selectively enrich respiring and non-respiring yeast cells, respectively, from a mixture of cells.
Skowronek
P
P
Institut für Pathologie der Universität Ulm, Federal Republic of Germany.
Krummeck
G
G
Haferkamp
O
O
Rödel
G
G
D005434:Flow Cytometry; D005456:Fluorescent Dyes; D008928:Mitochondria / Q000378:metabolism*; D010101:Oxygen Consumption; D020112:Rhodamine 123; D012235:Rhodamines; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
D005456:Fluorescent Dyes; D012235:Rhodamines; D020112:Rhodamine 123