title: The effect of anesthesia and surgery on diaphragmatic lymph vessel flow after endotoxin in sheep.
abstract: Increases in diaphragmatic lymph vessel flow (Qdi) may be important in preventing ascites because diaphragmatic lymph vessels drain the peritoneal space. However, lymphatic vessel function may be depressed in anesthetized, open chested animals. To test this hypothesis, we cannulated diaphragmatic lymph vessels in five sheep which were anesthetized with 1-2% halothane. We performed a thoracotomy and cannulated a diaphragmatic lymph vessel in each sheep. Then we infused 0.75-1.0 micrograms/kg of E. coli endotoxin intravenously and we measured Qdi and the lymph protein concentration for 2-4 hrs. The data were compared to previously reported data for five unanesthetized sheep (J. Appl. Physiol. 62:706-710, 1987). At baseline Qdi = 0.8 +/- 0.7 (SD) in the anesthetized sheep and 1.0 +/- 0.8 ml/hr in the unanesthetized sheep. After endotoxin, Qdi increased to 4.5 +/- 3.1 ml/hr in the unanesthetized sheep (p less than 0.05) but Qdi did not change in the anesthetized sheep. However, the lymph protein concentration increased similarly in each group, indicating that endotoxin caused the same degree of injury in each group. Our results indicate that diaphragmatic lymph vessel function is depressed in anesthetized, open chested sheep.
authors: Center for Microvascular and Lymphatic Studies, University of Texas Medical School, Houston.
authors: J R
authors: JR
authors: Elk
authors: T
authors: T
authors: Adair
authors: R E
authors: RE
authors: Drake
authors: J C
authors: JC
authors: Gabel
mesh_terms: D000704:Analysis of Variance; D000758:Anesthesia; D000818:Animals; D001201:Ascites / Q000503:physiopathology / Q000517:prevention & control; D001794:Blood Pressure; D003964:Diaphragm; D004731:Endotoxins / Q000494:pharmacology*; D004926:Escherichia coli; D007262:Infusions, Intravenous; D008208:Lymphatic System / Q000503:physiopathology* / Q000601:surgery; D011651:Pulmonary Artery / Q000502:physiology; D012756:Sheep
chemical_list: D004731:Endotoxins