Expression and chromosomal localization of a lymphocyte K+ channel gene.
We recently isolated a family of three closely related mouse K+ channel genes (MK1, MK2, and MK3) with coding regions contained in single uninterrupted exons. Here we have used patch-clamp recordings from Xenopus oocytes injected with mRNA to show that MK3 encodes a channel with biophysical and pharmacological properties indistinguishable from those of voltage-gated type n K+ channels in T cells. In addition, we used the polymerase chain reaction to demonstrate the presence of MK3 mRNA in T cells. These data suggest that MK3 may encode the T-cell voltage-gated type n K+ channel. We also show that MK3 and MK2 are localized on human chromosomes 13 and 12, respectively.
Grissmer
S
S
Department of Physiology and Biophysics, University of California, Irvine 92715.
Dethlefs
B
B
Wasmuth
J J
JJ
Goldin
A L
AL
Gutman
G A
GA
Cahalan
M D
MD
Chandy
K G
KG
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004594:Electrophysiology / Q000379:methods; D005260:Female; D008564:Membrane Potentials; D008969:Molecular Sequence Data; D005810:Multigene Family; D015345:Oligonucleotide Probes; D009865:Oocytes / Q000502:physiology; D015221:Potassium Channels / Q000235:genetics* / Q000502:physiology; D051381:Rats; D013601:T-Lymphocytes / Q000502:physiology*; D014981:Xenopus
D015345:Oligonucleotide Probes; D015221:Potassium Channels