Serologic, biologic, and western blot analysis of a T suppressor factor with specificity for the hapten 4-hydroxy-3-nitrophenyl acetyl derived from serum-free medium.
A T cell hybridoma producing a T suppressor factor (TsF) with specificity for the hapten nitrophenyl was converted to long term growth in serum-free medium and its product tested by serology, bioactivity, and Western blot analysis. Results indicated that Ag-specific suppressive activity was present in serum-free medium and this TsF could exhibit the characteristics ascribed to it by various groups: it could bind nominal Ag with specificity, it was bound by anti-TsF mAb, and it could mediate Ag-specific suppression both in vivo and in vitro. Western blot and SDS-PAGE analysis of this purified TsF revealed a 43-kDa single chain protein.
Gallina
M J
MJ
Department of Immunology, Scripps Clinic & Research Institute, La Jolla, CA 92037.
Kuchroo
V K
VK
Steele
J K
JK
D000818:Animals; D015153:Blotting, Western; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006241:Haptens / Q000276:immunology*; D006825:Hybridomas / Q000737:chemistry*; D006968:Hypersensitivity, Delayed; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D009596:Nitrophenols / Q000276:immunology*; D010648:Phenylacetates; D013491:Suppressor Factors, Immunologic / Q000032:analysis* / Q000276:immunology
D003470:Culture Media; D006241:Haptens; D009596:Nitrophenols; D010648:Phenylacetates; D013491:Suppressor Factors, Immunologic; C026242:4-hydroxy-5-nitrophenyl acetic acid