Identification of methionine enkephalin in the bovine cornea by fast atom bombardment-mass spectrometry.
Native methionine enkephalin and methionine enkephalin generated proteolytically from a larger peptide were both identified and quantified from bovine cornea by negative ion fast atom bombardment-mass spectrometry. The corneal peptides were purified initially be reverses-phase high performance liquid chromatography and identified tentatively by radioreceptor assay and/or radioimmunoassay.
Tinsley
P W
PW
Charles B. Stout Neuroscience Mass Spectrometry Laboratory, Memphis, TN.
Dass
C
C
Trimble
J W
JW
Desiderio
D M
DM
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003315:Cornea / Q000737:chemistry*; D004744:Enkephalin, Methionine / Q000032:analysis*; D011869:Radioligand Assay; D016339:Spectrometry, Mass, Fast Atom Bombardment
D004744:Enkephalin, Methionine