Genetic control of immunity to gastrointestinal nematodes of cattle.
Previous studies have indicated that host genetics significantly affects the number of gastrointestinal nematode eggs per gram (epg) in the feces of calves during their first grazing season. An entire calf crop of approximately 190 animals was monitored monthly until weaning to verify these earlier results, and to begin to discern the basis for this phenomenon. A significant genetic effect on fecal epg values was not observed until calves had been on pasture for 2-3 months, and was demonstrable until late in the grazing season when the effect was lost. The loss of a genetic effect coincided with the appearance of significant numbers of the more highly fecund nematode species Haemonchus placei and Oesophagostomum radiatum, and with an apparent increase in Ostertagia ostertagi transmission, indicating that the observed genetic control of epg values may be species specific, dose dependent or both. Calves were selected from the population, and grouped according to their epg phenotype over the grazing season as either high or low epg calves. Postmortem examination of some of these calves indicated that worm burdens in the low epg calves were 60% of those of the high epg calves. Experimental challenge inoculation of the remaining calves indicated that: (1) challenge with Cooperia oncophora resulted in low epg calves harboring worm numbers that were 65% of those of high epg calves; (2) challenge with O. ostertagi resulted in similar numbers of worms in both groups, but the fecundity of worms in the low epg groups was significantly lower (P less than 0.05) than in the high epg group. Analysis of serum anti-Ostertagia antibody levels in the grazing calf population showed rises in serum IgG1, IgG2, IgM and IgA antibody levels during the grazing season. Peak serum IgG2 and IgG1 anti-Ostertagia antibody levels were found to be significantly affected by host genetic factors while IgA and IgM levels were not under such control.
Gasbarre
L C
LC
USDA, ARS, LPSI, Helminthic Diseases Laboratory, Beltsville, MD 20705.
Leighton
E A
EA
Davies
C J
CJ
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D001947:Breeding; D002417:Cattle; D002418:Cattle Diseases / Q000235:genetics / Q000276:immunology*; D015331:Cohort Studies; D005243:Feces / Q000469:parasitology; D005260:Female; D007110:Immunity, Active / Q000235:genetics; D007136:Immunoglobulins / Q000096:biosynthesis; D007411:Intestinal Diseases, Parasitic / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D008297:Male; D009349:Nematode Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary*; D010028:Ostertagia / Q000276:immunology / Q000302:isolation & purification; D010029:Ostertagiasis / Q000235:genetics / Q000276:immunology / Q000662:veterinary; D010270:Parasite Egg Count / Q000662:veterinary
D000909:Antibodies, Helminth; D007136:Immunoglobulins