Structure and chromosomal location of the human gene encoding cartilage matrix protein.
Cartilage matrix protein (CMP) is a major component of the extracellular matrix of nonarticular cartilage. The structure and chromosomal location of the human gene encoding CMP was determined by molecular cloning analysis. We used a partial chicken CMP cDNA probe to isolate three overlapping human genomic clones. From one of these clones, a probe containing 2 human CMP exons was isolated and used to map the gene to chromosome 1p35 and to screen a human retina cDNA library. Two overlapping cDNA clones were isolated. The predicted protein sequence of 496 amino acids includes a 22-residue signal peptide and a 474-residue mature protein of Mr 51,344. The human CMP gene and polypeptide are strikingly similar to the chicken CMP gene and polypeptide. Human CMP is 79% identical to chicken CMP and contains two homologous domains separated by an epidermal growth factor-like domain. One potential N-glycosylation site is conserved between the two species. The human CMP gene spans 12 kilobase pairs with 8 exons and 7 introns which are similar in size to those of the chicken CMP gene. Both RNA splice junctions of intron G in the human and chicken CMP genes are nonconforming to the consensus splice sequences. This suggests that the CMP gene utilizes a new RNA splicing mechanism.
Jenkins
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Harold C. Simmons Arthritis Research Center, Dallas, Texas.
Osborne-Lawrence
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Sinclair
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Eddy
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RL
Byers
M G
MG
Shows
T B
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Duby
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AD
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D064236:Cartilage Oligomeric Matrix Protein; D002645:Chickens; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D015342:DNA Probes; D005091:Exons; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins / Q000235:genetics*; D006031:Glycosylation; D006801:Humans; D007438:Introns; D064235:Matrilin Proteins; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics; D014176:Protein Biosynthesis; D012326:RNA Splicing; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D064236:Cartilage Oligomeric Matrix Protein; D015342:DNA Probes; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D064235:Matrilin Proteins; C577553:TSP5 protein, human