title: Flow cytometric studies on actin polymerization in PMN cells from chronic myeloid leukemia (CML) patients.
abstract: Studies in our laboratory have shown that polymorphonuclear leucocytes (PMNL) from chronic myeloid leukemia (CML) patients are defective in chemotaxis towards a synthetic peptide, n-formyl-methionyl-leucyl-phenylalanine (FMLP), during the active phases of the disease and in remission. Actin plays a major role in cellular movements and binding of chemo-attractant to cells induces polymerization of G-actin to F-actin. We have, therefore, compared polymerization of actin in FMLP stimulated PMNL from CML patients with those from normal subjects by fluorescence microscopy and flow cytometry, using F-actin specific probe, NBD-phallacidin. Our results show that binding of FMLP to normal PMNL induces rapid conversion of G-actin to F-actin followed by depolymerization to some extent. In CML PMNL, such a biphasic response is not seen. Conversion of G-actin to F-actin is slower and F-actin content is significantly lower than that in normal PMNL. Moreover, organization of F-actin is different in CML PMNL as compared to that in normal PMNL.
authors: Cancer Research Institute, Tata Memorial Centre, Parel, Bombay, India.
authors: N R
authors: NR
authors: Naik
authors: A N
authors: AN
authors: Bhisey
authors: S H
authors: SH
authors: Advani
mesh_terms: D000199:Actins / Q000378:metabolism*; D000546:Amanitins; D005434:Flow Cytometry; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood*; D008856:Microscopy, Fluorescence; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000378:metabolism / Q000494:pharmacology; D009504:Neutrophils / Q000378:metabolism*; D011108:Polymers
chemical_list: D000199:Actins; D000546:Amanitins; D011108:Polymers; D009240:N-Formylmethionine Leucyl-Phenylalanine; C028877:7-nitrobenz-2-oxa-1,3-diazole-phallacidin