[An experimental study on preparing monoclonal antibodies of rough Brucella spp. and identification species Brucella].
A report of preparation of monoclonal antibodies of B. canis and B. ovis and the use of identification for rough species of Brucella was described. The results showed that the above two monoclonal antibodies were specific for rough Brucella Spp. The use of the above described monoclonal antibodies gave us the possibility to differentiate rough and smooth Brucella species. The results also showed that the ELISA using monoclonal antibodies of rough Brucella Spp. was more specific than the agglutination test using routine antibodies of rough Brucella.
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Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000942:Antigens, Bacterial / Q000032:analysis; D002002:Brucella / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial