Inhibition of gap-junctional intercellular communication and enhanced binding of fibronectin-coated latex beads by stimulation of DNA synthesis in quiescent 3T3-L1 cells.
To clarify the modulation of intercellular communication via gap junctions, associated with the growth induction of quiescent 3T3-L1 cells, we investigated the gap-junctional intercellular communication in growth-stimulated cells that were able to bind fibronectin-coated beads. When quiescent 3T3-L1 cells were incubated with fibronectin-coated beads for the first 2 h after the addition of calf serum, 24.0% of the cells bound and phagocytosed beads. Among the cells with bound beads, the percentage of the cells labeled concurrently with bromodeoxyuridine was 63.7% when examined 13 h after the addition of calf serum. Transient reduction of dye-coupling, measured with Lucifer Yellow CH, was observed only in the cells with bound beads 2 h after addition of calf serum, but it was not observed in the cells without bound beads. When the quiescent cells were incubated with fibronectin-coated beads for 2 h from 4-6 h after the addition of calf serum, the percentage of cells with bound beads increased to 53.1%, but the decrease in dye-coupling among the cells with bound beads was slight. These results suggest that the induction of cell growth causes a transient reduction in gap-junctional intercellular communication in 3T3-L1 cells with bound fibronectin-coated beads.
Shiba
Y
Y
Department of Oral Physiology, Hiroshima University School of Dentistry, Japan.
Sasaki
Y
Y
Kanno
Y
Y
D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002450:Cell Communication / Q000502:physiology*; D002455:Cell Division / Q000502:physiology*; D002478:Cells, Cultured; D004261:DNA Replication / Q000502:physiology*; D005353:Fibronectins / Q000378:metabolism*; D005456:Fluorescent Dyes; D016193:G1 Phase / Q000502:physiology; D007365:Intercellular Junctions / Q000502:physiology*; D007840:Latex; D008845:Microinjections; D016192:Resting Phase, Cell Cycle / Q000502:physiology; D013997:Time Factors
D005353:Fibronectins; D005456:Fluorescent Dyes; D007840:Latex; D001973:Bromodeoxyuridine