Protonated triplex DNA in E. coli cells as detected by chemical probing.
The triplex structure in vitro is well established; however, no direct evidence has been available concerning its existence in the cell. Using the direct chemical probing here we show that the triplex H structure can exist in E. coli cells at acidic intracellular pH values; this structure differs in some details from that observed in vitro.
Karlovsky
P
P
Institute of Biophysics, Czechoslovak Academy of Sciences, Brno.
Pecinka
P
P
Vojtiskova
M
M
Makaturova
E
E
Palecek
E
E
D001483:Base Sequence; D001665:Binding Sites; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics*; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009992:Osmium; D010957:Plasmids; D015183:Restriction Mapping
D004269:DNA, Bacterial; D046911:Macromolecular Substances; D009992:Osmium