The effects of different routes of injection on the biochemistry of adhesion site proteoglycans in metastatic v-Ki-ras-transformed 3T3 cells.
This study compared HSPG and CSPG in adhesion sites of isogenic metastatic lung tumor cells (v-Ki-ras-transformed 3T3 cells) after injection by different routes (IV and footpad) into athymic nude mice. The proportion of HSPG was significantly greater in experimental lung metastatic cell (IV injection) than in spontaneously metastatic cells. The proportion of CSPG in these two groups of metastatic lung cells did not differ significantly. In addition, the proportion of HSPG and CSPG in spontaneously metastatic lung tumor cells was similar, unlike experimental metastatic tumor cells.
Kosir
M A
MA
Department of Surgery, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106.
Houmiel
K L
KL
Culp
L A
LA
D000818:Animals; D002448:Cell Adhesion; D002461:Cell Line, Transformed; D011508:Chondroitin Sulfate Proteoglycans / Q000032:analysis*; D011905:Genes, ras; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate / Q000032:analysis*; D007267:Injections; D008175:Lung Neoplasms / Q000737:chemistry* / Q000556:secondary; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude
D011508:Chondroitin Sulfate Proteoglycans; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate