Oncogenes in multiple myeloma: point mutation of N-ras.
Alterations of ras, c-myc and bcl-1 have been described in hematologic malignancies of lymphoid origin. We investigated the structure of these genes and evaluated the frequency of point mutations involving H-, K- or N-ras in bone marrow samples from patients with multiple myeloma. No abnormalities were detected in the c-myc and bcl-1 genes, but two of 17 patients were found to have N-ras mutations by differential oligonucleotide hybridization and dideoxynucleotide sequencing following amplification by polymerase chain reaction. Bone marrow DNA from both patients had identical missense mutations of N-ras codon 61 changing CAA to AAA, resulting in a substitution of lysine for glutamine in the encoded protein. Multiple myeloma is the first mature B cell neoplasm found to harbor ras mutations.
Paquette
R L
RL
Division of Hematology/Oncology, UCLA 90024.
Berenson
J
J
Lichtenstein
A
A
McCormick
F
F
Koeffler
H P
HP
D001483:Base Sequence; D001853:Bone Marrow / Q000737:chemistry / Q000473:pathology; D011905:Genes, ras; D006801:Humans; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D009154:Mutation; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D009857:Oncogenes; D010950:Plasma Cells / Q000473:pathology; D016133:Polymerase Chain Reaction
D015345:Oligonucleotide Probes