[The use of monoclonal antibodies with human IgE for determining the level of allergen-specific IgE].
The possibility of using monoclonal antibodies to human IgE, obtained in our laboratory, with the aim to determine allergen-specific IgE in the radioallergosorbent test (RAST), the enzyme immunoassay and the radioimmunoassay has been studied. The results obtained with the use of the standard RAST system (manufactured by Pharmacia, Sweden) and different variants of assay based on the use of our antibodies, labeled with 125I or conjugated with peroxidase, and timothy pollen allergen (obtained from the Stavropol Research Institute for Vaccines and Sera) have been compared. The best results have been obtained with anti-IgE antibodies 11-2, but for working out our assay systems it is necessary to develop our own standards and control limits.
Smirnova
E Ia
EIa
Lebedin
Iu S
IuS
Serdiuk
O A
OA
Vasilov
R G
RG
D000485:Allergens / Q000276:immunology*; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity / Q000276:immunology*; D002138:Calibration; D006801:Humans; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E / Q000032:analysis / Q000276:immunology*; D011852:Radioallergosorbent Test / Q000379:methods / Q000592:standards
D000485:Allergens; D000911:Antibodies, Monoclonal; D007073:Immunoglobulin E
Ispol'zovanie monoklonal'nykh antitel k IgE cheloveka dlia opredeleniia urovnia allergenspetsificheskogo IgE.