title: Direct evidence for the involvement of vasoactive intestinal polypeptide in the motor response of the human isolated ileum to capsaicin.
abstract: Capsaicin (1 microM) produced complex motor responses in longitudinal and circular muscle strips from the human isolated small intestine (jejunum and ileum). In the longitudinal muscle, inhibition of the nerve-mediated contractions (electrical field stimulation) was the dominant response, while capsaicin had a weak and inconsistent effect on tone and spontaneous activity. In contrast, relaxation and decreased spontaneous activity were the responses of the circular muscle to capsaicin. These effects of capsaicin were not reproduced by a second application of capsaicin, indicating desensitization, a feature of the specific action of this drug on sensory nerves. All the effects of capsaicin in the longitudinal and circular muscle were closely mimicked by exogenous vasoactive intestinal polypeptide (VIP). Further, the inhibitory motor effect of capsaicin in both muscle layers was blocked by an anti VIP serum. In the longitudinal muscle, VIP, like capsaicin, inhibited the electrically evoked nerve-mediated contractions but not the tetrodotoxin-resistant myogenic contractions, suggesting a prejunctional site of action. The inhibitory effect of both capsaicin and VIP in the circular muscle was tetrodotoxin-resistant suggesting direct inhibition of muscle cells. Capsaicin (1 microM) evoked a tetrodotoxin-resistant release of VIP-like immunoreactivity from the human small intestine. On high pressure liquid chromatography, a major peak of the immunoreactive material released by capsaicin co-eluted with authentic VIP and a minor, unidentified peak eluted shortly afterward. We conclude that authentic VIP is involved in the local motor response to capsaicin in the human small intestine. These findings raise the possibility that VIP might be present in sensory nerves of the human gut from which it is released by capsaicin.
authors: Pharmacology Department, A. Menarini Pharmaceuticals, Florence, Italy.
authors: C A
authors: CA
authors: Maggi
authors: S
authors: S
authors: Giuliani
authors: P
authors: P
authors: Santicioli
authors: R
authors: R
authors: Patacchini
authors: S I
authors: SI
authors: Said
authors: E
authors: E
authors: Theodorsson
authors: D
authors: D
authors: Turini
authors: G
authors: G
authors: Barbanti
authors: A
authors: A
authors: Giachetti
authors: A
authors: A
authors: Meli
mesh_terms: D000368:Aged; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology; D002211:Capsaicin / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004558:Electric Stimulation; D006801:Humans; D007082:Ileum / Q000187:drug effects; D066298:In Vitro Techniques; D007583:Jejunum / Q000187:drug effects; D008875:Middle Aged; D009119:Muscle Contraction / Q000187:drug effects; D009126:Muscle Relaxation / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects*; D009474:Neurons / Q000502:physiology; D013779:Tetrodotoxin / Q000494:pharmacology; D014660:Vasoactive Intestinal Peptide / Q000276:immunology / Q000502:physiology*
chemical_list: D014660:Vasoactive Intestinal Peptide; D013779:Tetrodotoxin; D015740:Calcitonin Gene-Related Peptide; D002211:Capsaicin