Characterization of a DNA binding protein of bacteriophage PRD1 involved in DNA replication.
Escherichia coli phage PRD1 protein P12, involved in PRD1 DNA replication in vivo, has been highly purified from E. coli cells harbouring a gene XII-containing plasmid. Protein P12 binds to single-stranded DNA as shown by gel retardation assays and nuclease protection experiments. Binding of protein P12 to single-stranded DNA increases about 14% the contour length of the DNA as revealed by electron microscopy. Binding to single-stranded DNA seems to be cooperative, and it is not sequence specific. Protein P12 also binds to double-stranded DNA although with an affinity 10 times lower than to single-stranded DNA. Using the in vitro phage phi 29 DNA replication system, it is shown that protein P12 stimulates the overall phi 29 DNA replication.
Pakula
T M
TM
Department of Genetics, University of Helsinki, Finland.
Caldentey
J
J
Serrano
M
M
Gutierrez
C
C
Hermoso
J M
JM
Salas
M
M
Bamford
D H
DH
D003090:Coliphages / Q000187:drug effects / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects*; D004277:DNA, Single-Stranded / Q000378:metabolism; D004279:DNA, Viral / Q000096:biosynthesis*; D004268:DNA-Binding Proteins / Q000235:genetics*; D009690:Nucleic Acid Conformation; D011487:Protein Conformation; D014764:Viral Proteins / Q000494:pharmacology*
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D014764:Viral Proteins; D004247:DNA