Flow cytometric detection of ornithine decarboxylase activity in epidermal cell subpopulations.
Using flow cytometry in combination with membrane permeabilization techniques to enhance binding of antibodies with immunoreactive protein within the cytoplasm, we have developed a method to examine the ornithine decarboxylase (ODC) activity present within subpopulations of epidermal cells following acute and chronic exposure to the phorbol ester tumor promoter 12-0-tetradecanoylphorbol-13-acetate (TPA). The method described has the sensitivity to detect basal levels of ODC as well as increases in ODC at early time points following treatment with TPA and has the additional advantage of allowing subpopulation identification and characterization.
Robertson
F M
FM
Department of Surgery, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, New Brunswick 08903.
Gilmour
S K
SK
Beavis
A J
AJ
O'Connell
S M
SM
Conney
A H
AH
Huang
M T
MT
Laskin
J D
JD
Hietala
O A
OA
O'Brien
T G
TG
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D002478:Cells, Cultured; D000078404:Epidermal Cells; D004817:Epidermis / Q000187:drug effects / Q000201:enzymology; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry / Q000379:methods; D015603:Keratinocytes / Q000187:drug effects / Q000201:enzymology; D051379:Mice; D009955:Ornithine Decarboxylase / Q000276:immunology / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000906:Antibodies; D009955:Ornithine Decarboxylase; D013755:Tetradecanoylphorbol Acetate