[Assessment of the content of small peptides in enzymatic proteolysates using a cuprimetric method: value in nutritional medicine].
The nutritional interest of dipeptides and tripeptides is more and more recognized nowadays. Directly absorbed by enterocyte, they enable to improve the transfer of amino acids not easily absorbable. The quality of proteins hydrolysates mainly depends on the importance of their concentration. But official methods of analysis of protein hydrolysates (total nitrogen, alpha amino-nitrogen) are inept to estimate their concentrations. It is possible to realize this estimation by grounding on reactivity with Cu II. Indeed like free amino acids they can give complexes with this ion. The proposed cuprimetric method loads to a more sensible estimation, and especially it's possible to differentiate homogeneous hydrolysates from mixtures which have been added with amino acids. Theirs it enables the nutritional physician to have a better knowledge of the biodisponibility of this preparation.
Hamon
M
M
Service de Pharmacie, Hôpital Necker-Enfants Malades, Paris.
D003300:Copper; D005069:Evaluation Studies as Topic; D006801:Humans; D009747:Nutritional Physiological Phenomena; D010455:Peptides / Q000032:analysis*; D011492:Protein Hydrolysates / Q000032:analysis*
D010455:Peptides; D011492:Protein Hydrolysates; D003300:Copper
Appréciation de la teneur en petits peptides de protéolysats enzymatiques à l'aide d'une méthode cuprimétrique: intérêt en médecine nutritionnelle.