Analysis of the changes in the structure and hydration of the nucleosome core particle at moderate ionic strengths.
In order to better understand the conformational changes induced in the nucleosome core particle by changes in the ionic strength of the media in the range from 0.1 to 0.6 M NaCl, we have conducted a very detailed structural analysis, combining circular dichroism, DNase I digestion, and sedimentation equilibrium. The results of such analysis indicate that the secondary structure of both DNA and histones exhibits small (approximately 5%) but noticeable changes as the salt increases within this range. In the case of DNA, the data are consistent with a trend toward a more relaxed secondary structure. The DNase I pattern of digestion is also altered by the salt and suggests a DNA relaxation around the flanking ends. From the hydrodynamic measurements, we also observe a significant change in the virial coefficients of the particle as the salt increases, which in turn are in very good agreement with the theoretically expected values. Furthermore, the preferential hydration parameter is also found to increase with the salt. We believe that the self-dependent conformational change of the nucleosome core particle is the result of the conjunction of all these subtle changes. Yet, from the present data, their exact relationship to the tertiary structure of the whole particle at the different ionic strengths cannot be exactly defined.
Dong
F
F
Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331-6503.
Nelson
C
C
Ausio
J
J
D002942:Circular Dichroism; D003850:Deoxyribonuclease I; D008956:Models, Chemical; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000737:chemistry*; D009994:Osmolar Concentration; D011487:Protein Conformation; D012965:Sodium Chloride; D014867:Water
D009707:Nucleosomes; D014867:Water; D012965:Sodium Chloride; D003850:Deoxyribonuclease I