Effects of rat and chicken calcitonin gene-related peptides (CGRP) upon calcium metabolism in chicks.
In vivo effects of intravenously injected chicken(c-) and rat(r-) calcitonin gene related peptide (CGRP) upon plasma total (Cat), ionized (Cai) calcium, inorganic phosphate (Pi) and clearance of an acutely administered 45Ca label have been examined in chicks. Both peptides were hypercalcaemic in fasted chicks, unlike previously reported hypocalcaemic response in mammals. r-CGRP was hypercalcaemic at doses of both 0.26 and 1.31 nmol/100 g body wt, the lower dose produced a significant elevation of Cat one hour after injection into 12-h-fasted chicks, the upper dose had a similar effect at 20 min. Cai was also non-significantly elevated by r-CGRP. Pi was slightly increased by r-CGRP at both doses, 20 and 60 min after injection. c-CGRP produced a dose (0.26-4.17 nmol/100 g body wt) dependent elevation of Cat and Cai in 22-h-fasted chicks. A greater response was however seen in fed animals. Peak responses were observed 45 min after injection. c-CGRP (1.04 nmol/100 g body wt) caused a significant decline in plasma Pi (P less than 0.05) in fasted chicks. Pi was elevated in control fed animals compared with fasted controls. c-CGRP (1.04 nmol/100 g) did not effect plasma Pi in fed chicks. Whilst both peptides elevated plasma Ca, clearance of an acutely administered 45Ca label from plasma was greater in both r-CGRP treated 12-h-fasted chicks and c-CGRP treated 22-h-fasted chicks. In contrast, the rate of 45Ca clearance in fed chicks was not affected by c-CGRP treatment. The differential effects of these peptides upon plasma 45Ca clearance and other plasma parameters of Ca metabolism, suggest a complex mode of action of the peptide upon avian Ca homeostasis, possibly involving direct actions upon kidney and bone.
Ancill
A K
AK
Division of Pharmacology, School of Pharmacy, Portsmouth Polytechnic, U.K.
Bascal
Z A
ZA
Whitaker
G
G
Dacke
C G
CG
D000818:Animals; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism; D015740:Calcitonin Gene-Related Peptide / Q000008:administration & dosage / Q000494:pharmacology*; D002118:Calcium / Q000097:blood / Q000378:metabolism*; D002645:Chickens; D004305:Dose-Response Relationship, Drug; D006934:Hypercalcemia / Q000139:chemically induced; D010710:Phosphates / Q000378:metabolism; D051381:Rats; D013045:Species Specificity
D010710:Phosphates; D015740:Calcitonin Gene-Related Peptide; D002118:Calcium