[Effects of three kinds of inorganic dusts on lipid peroxidation of erythrocytes].
This paper reports on the effects of erythrocytes when incubated with three kinds of inorganic dusts, i.e. quartz (free SiO2 content 97%), alpha-Al2O3 and TiO2. Malonaldehyde as a product of lipid peroxidation was determined as it reacted with thiobarbituric acid, and haemolytic activity was analysed spectrophotometrically. The results showed that quartz presented a stronger stimulative effect on lipid peroxidation of erythrocyte at doses of 1-8 mg than the control (P less than 0.01). No change was observed with alpha-Al2O3 and TiO2 at doses of 1-9 mg. The results suggest that the effects of dusts on lipid peroxidation of erythrocyte are similar to hemolytic reaction, directly reflecting the degree of their damage to the cell membrane. Therefore lipid peroxidation on cell membrane caused by dusts was one of the parameters of cytotoxic effects, and could be considered as one of the measures used in the assessment of cytotoxicity of dusts.
Sun
M S
MS
Institute of Labour Hygiene and Occupational Diseases of Heilongjiang Province, Harbin.
D000537:Aluminum Oxide / Q000009:adverse effects; D000818:Animals; D004391:Dust / Q000009:adverse effects*; D004912:Erythrocytes / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008315:Malondialdehyde / Q000378:metabolism; D011791:Quartz / Q000009:adverse effects*; D051381:Rats; D011919:Rats, Inbred Strains; D006435:Renal Dialysis; D014025:Titanium / Q000009:adverse effects
D004391:Dust; D011791:Quartz; C009495:titanium dioxide; D008315:Malondialdehyde; D014025:Titanium; D000537:Aluminum Oxide