[Comparative accumulation of the Hoechst 33258 fluorescent probe in leukemia P388 cells sensitive and resistant to doxorubicin].
The authors studied accumulation of the fluorescent probe Hoechst 33258 in leukemia P 388 sensitive (P 388/0) and resistant to doxorubicin (P 388/DOX) cells. It was shown that intensity of fluorescence of the dye increased after binding with nuclear DNA during 25 min for both lines of the cells. Intensity of fluorescence was 40% greater in sensitive than resistant cells. If Triton X-100 was added no difference between two lines of the cell was observed. When doxorubicin was added to the cells with dye, the intensity of fluorescence decreased. It was suggested to use Hoechst 33258 for assessment extent doxorubicin accumulation in nuclei of the cells.
Donenko
F V
FV
Borovkova
N B
NB
Egudina
S V
SV
Kabieva
A O
AO
Kris'ko
Sh I
ShI
Moroz
L V
LV
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D004317:Doxorubicin / Q000493:pharmacokinetics / Q000627:therapeutic use*; D004351:Drug Resistance; D004354:Drug Screening Assays, Antitumor; D005456:Fluorescent Dyes; D007941:Leukemia P388 / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D051379:Mice; D017830:Octoxynol; D011092:Polyethylene Glycols; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D011092:Polyethylene Glycols; D004317:Doxorubicin; D017830:Octoxynol
Issledovanie sravnitel'nogo nakopleniia fliuorestsentnogo zonda Khekhst 33258 v kletkakh leĭkoza P 388, chuvstvitel'nykh i ustoĭchivykh k doksorubitsinu.