Application of micro-scale liquid chromatography with fluorescence detection to the determination of thiols.
Different thiol compounds of biological and pharmacological interest were separated on a packed reversed-phase fused-silica capillary column and determined with fluorescence detection. Conventional inexpensive liquid chromatographic equipment could be adapted for such purposes, providing a highly efficient analytical system. The different compounds were derivatized with the fluorogenic reagents SBD-F and ABD-F and chromatographed both isocratically and in the gradient mode in order to separate a series of thiols with widely varying polarities. Subsequently the derivatives were measured at lambda ex. = 380 nm and lambda em. = 510 nm. Application of the system to biological and pharmacological samples is suggested.
Ling
B L
BL
Department of Pharmaceutical Chemistry and Drug Quality Control, State University of Ghent, Belgium.
Dewaele
C
C
Baeyens
W R
WR
D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D007202:Indicators and Reagents; D008832:Microchemistry; D013050:Spectrometry, Fluorescence; D013438:Sulfhydryl Compounds / Q000032:analysis* / Q000097:blood
D007202:Indicators and Reagents; D013438:Sulfhydryl Compounds