A hypotensive procyanidin-glycoside from Rhamnus lycioides ssp. lycioides.
A lyophilized hot water extract of the aerial parts of Rhamnus lycioides L. (Rhamnaceae) produced a lowering of systemic arterial blood pressure in normotensive anaesthetized Wistar rats. An activity-guided fractionation of the methanolic extract led to the isolation of a tetrameric procyanidin-glycoside which produced a clear dose-dependent hypotensive response (1.5-6 mg/kg i.v.). This principle was characterized using acid hydrolysis, thiolytic degradation and spectroscopic methods. It consisted of four flavanol units with a 2,3-cis configuration and with a O-beta-d-glucosylpyranoside function on the epicatechin terminal unit. The interflavan linkage was (4-8).
Terencio
M C
MC
Departamento de Farmacología y Farmacotecnia, Facultad de Farmacia, Universitat de València, Spain.
Sanz
M J
MJ
Payá
M
M
D000818:Animals; D044946:Biflavonoids; D001794:Blood Pressure / Q000187:drug effects*; D002247:Carbon Isotopes; D002392:Catechin / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D007022:Hypotension / Q000139:chemically induced*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D010944:Plants / Q000032:analysis; D010946:Plants, Medicinal; D010947:Plants, Toxic; D044945:Proanthocyanidins; D051381:Rats; D011919:Rats, Inbred Strains; D031955:Rhamnus / Q000032:analysis*
D044946:Biflavonoids; D002247:Carbon Isotopes; D006027:Glycosides; D010936:Plant Extracts; D044945:Proanthocyanidins; C017674:procyanidin; D002392:Catechin