NACM, a cytopathogenic protein from Naegleria gruberi, EGs; purification, production of monoclonal antibody, and the immunoidentification of a product that develops in NACM-treated vertebrate cell cultures.
The story of NACM involves the discovery of a deleterious response of cultured vertebrate cells to a component in cell-free lysates prepared from free-living amebae of the genus Naegleria; hence the acronym NACM derived from Naegleria ameba cytopathogenic material. The cellular reaction is the basis for the biological assay that has been fundamental in the study of the action of NACM in a variety of cell cultures. It also has been used in the determination of the physical characteristics, and to monitor the behavior of NACM during isolation procedures. All findings are compatable with the conclusion that NACM is a 35 Kd protein. Recently, the use of monoclonal antibodies (MAbs) prepared to amebae-derived purified NACM have resulted in the visual display of a product that develops exclusively in NACM-treated cells. That cellular product is shown to be related to NACM by its immunostaining reaction with the MAb; the relationship of the MAb with NACM is demonstrated by its ability to neutralize the biological activity of NACM, and as an immunostain, to react with purified fractions of NACM and with whole amebae. The combination of these observations describes a unique set of interactions in which NACM, an amebic component, identified as a protein, has characteristics of an infectious agent when introduced into cultures of avian and mammalian cells.
Dunnebacke
T H
TH
Viral and Rickettsial Disease Laboratory, State of California Department of Health Services, Berkeley 94704.
Dixon
J S
JS
D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D009253:Naegleria / Q000032:analysis*; D015800:Protozoan Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000633:toxicity*; D014118:Toxins, Biological / Q000096:biosynthesis / Q000302:isolation & purification*; D014714:Vertebrates
D000911:Antibodies, Monoclonal; D015800:Protozoan Proteins; D014118:Toxins, Biological