N-3 polyunsaturated fatty acids enhance cholesterol efflux from human fibroblasts in culture.
Normal human skin fibroblasts were incubated in medium supplemented with 60 micrograms/ml linoleic acid (18:2n6) or eicosapentaenoic acid (20:5n3). After five days, cells lipids were enriched with linoleic acid or with docosapentaenoic acid (22:5n3). The HDL-mediated efflux of cholesterol from cells enriched with n-3 polyunsaturated fatty acids (PUFAs) was twice as fast as the rate of efflux of cholesterol from cells enriched with n-6 PUFAs. This suggests that the fatty acid composition of cellular lipids affects cholesterol efflux. The faster efflux when cells contain n-3 PUFAs may account for part of the reduction in risk of coronary disease with increases in dietary n-3 PUFAs (fish oils).
Pal
S
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Department of Biochemistry, Memorial University of Newfoundland, St. John's, Canada.
Davis
P J
PJ
D002478:Cells, Cultured; D008076:Cholesterol, HDL / Q000378:metabolism*; D003327:Coronary Disease / Q000517:prevention & control; D004041:Dietary Fats / Q000494:pharmacology*; D015118:Eicosapentaenoic Acid / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology; D010743:Phospholipids / Q000378:metabolism; D012307:Risk Factors
D008076:Cholesterol, HDL; D004041:Dietary Fats; D008041:Linoleic Acids; D010743:Phospholipids; D019787:Linoleic Acid; D015118:Eicosapentaenoic Acid