Solubilisation of a cell wall bound invertase in Aspergillus nidulans.
Aspergillus nidulans produces an extracellular invertase when incubated in the presence of sucrose and about half of the activity produced was found to be associated with the mycelium. Sixty percent of this mycelial invertase could be solubilised by simple mechanical disruption. Among the agents tested for solubilisation of invertase, proteinase K and dithiothreitol were the most effective.
Vainstein
M H
MH
Department of Botany, University of Nottingham, U.K.
Peberdy
J F
JF
D001233:Aspergillus nidulans / Q000201:enzymology*; D002473:Cell Wall / Q000737:chemistry* / Q000187:drug effects; D002918:Chymotrypsin / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D019286:Endopeptidase K; D000431:Ethanol / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000378:metabolism*; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D011136:Polysorbates / Q000494:pharmacology; D012697:Serine Endopeptidases / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology; D043324:beta-Fructofuranosidase
D011136:Polysorbates; D012967:Sodium Dodecyl Sulfate; D000431:Ethanol; D011092:Polyethylene Glycols; D014508:Urea; D017830:Octoxynol; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; D012697:Serine Endopeptidases; D002918:Chymotrypsin; D019286:Endopeptidase K; D004229:Dithiothreitol