Constitutive and inducible expression of a transgene directed by heterologous promoters in a trout liver cell line.
Activities of heterologous promoters and enhancers in cultured rainbow trout liver cells were examined employing the bacterial chloramphenicol acetyltransferase gene as the reporter. SV40 promoter-enhancer and Rous sarcoma virus long terminal repeat directed constitutive expression at high levels. Moloney murine leukemia virus long terminal repeat and SV40 promoter combined with Adenovirus type 2 enhancer were also constitutively expressed. Drosophila Hsp70 promoter was activated when the transformed cells were cultured at 25 degrees C, a higher temperature than the temperature normally used, in faithful response to heat shock.
Inoue
K
K
Central Research Laboratory, Nippon Suisan Kaisha, Ltd., Tokyo, Japan.
Akita
N
N
Yamashita
S
S
Shiba
T
T
Fujita
T
T
D000818:Animals; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000235:genetics; D004330:Drosophila / Q000235:genetics; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000235:genetics*; D008099:Liver / Q000737:chemistry*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D014170:Transformation, Genetic; D014337:Trout / Q000235:genetics*
D006360:Heat-Shock Proteins; D015500:Chloramphenicol O-Acetyltransferase