Detection of single-stranded DNA in scrapie-infected brain by electron microscopy.
The nucleic acid content of enriched preparations of mitochondria/tubulofilamentous particles from normal and scrapie-infected hamster brains were examined by electron microscopy. After spreading on collodion-coated grids circular molecules of approximately 15.7 kb corresponding in size to mitochondrial DNA (mtDNA) were observed both in normal and scrapie-infected brains. In nucleic acid preparations from scrapie-infected brains multimeric mtDNA and single-stranded DNA strands of about 0.49 X 10(6) daltons were also visualized. These findings demonstrate the presence of a single-stranded DNA in scrapie-infected brains and are consistent with previous data based on enzyme digestion of nucleic acids isolated from scrapie-infected brains.
Narang
H K
HK
Institute of Pathology, General Hospital, Newcastle Upon Tyne, England.
D000818:Animals; D001921:Brain / Q000473:pathology; D001923:Brain Chemistry; D006224:Cricetinae; D004277:DNA, Single-Stranded / Q000032:analysis* / Q000648:ultrastructure; D005260:Female; D008647:Mesocricetus; D008854:Microscopy, Electron / Q000379:methods; D012608:Scrapie / Q000235:genetics*
D004277:DNA, Single-Stranded