title: Effects of androgens on parathyroid hormone and interleukin-1-stimulated prostaglandin production in cultured neonatal mouse calvariae.
abstract: In this study we show direct inhibitory effects on prostaglandin E2 (PGE2) production by the androgens, testosterone (T) and dihydrotestosterone (DHT), in cultured neonatal mouse calvariae. After 24 h of preculture with or without androgens, bones were treated with bovine (1-34)-parathyroid hormone (PTH) or recombinant human interleukin-1 alpha (IL-1). During preculture androgens decreased PGE2 release only in those experiments in which control PGE2 was high. PTH increased medium PGE2 9-fold at 24 h, and 10(-11) M T inhibited this increase by 50%. Treatment with IL-1 for 24 h increased medium PGE2 19- to 22-fold, and 10(-10) M T and DHT inhibited this increase by 60 and 70%, respectively. T did not significantly affect the PTH-stimulated release of previously incorporated 45Ca or alter the PTH inhibition of incorporation of [3H]proline into collagenase-digestible protein. IL-1 stimulated 45Ca release by 60-80%, and small but significant reductions of 20-30% were seen with T and DHT. This study shows that T and DHT have direct effects on bone at physiologic concentrations, similar to our previous study in which PTH-stimulated PGE2 production in the same culture system was inhibited by physiologic concentrations of 17 beta-estradiol, and suggests that prostaglandins may mediate some of the effects of androgens in vivo.
authors: Department of Medicine, University of Connecticut Health Center, Farmington 06032.
authors: C C
authors: CC
authors: Pilbeam
authors: L G
authors: LG
authors: Raisz
mesh_terms: D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D001842:Bone and Bones / Q000187:drug effects* / Q000378:metabolism; D046508:Culture Techniques; D013196:Dihydrotestosterone / Q000494:pharmacology; D015232:Dinoprostone / Q000096:biosynthesis*; D007375:Interleukin-1 / Q000494:pharmacology; D051379:Mice; D010281:Parathyroid Hormone / Q000494:pharmacology; D013739:Testosterone / Q000494:pharmacology
chemical_list: D000728:Androgens; D007375:Interleukin-1; D010281:Parathyroid Hormone; D013196:Dihydrotestosterone; D013739:Testosterone; D015232:Dinoprostone