Two naturally-occurring isoforms and their expression of a glucocorticoid receptor gene from an androgen-dependent mouse tumor.
We have isolated cDNAs encoding the glucocorticoid receptor from an androgen-dependent mouse tumor, Shionogi Carcinoma 115. The nucleotide sequence of the receptor revealed two different forms, designated as SC-GR I and SC-GR II. Both forms have a one-base substitution in the DNA binding domain of the wild-type mouse glucocorticoid receptor. Furthermore, SC-GR II has a three-base insertion in the interfinger region of this domain. By expressing the receptor cDNAs in cultured cells with MMTV-CAT reporter plasmid, SC-GR II was found to have about half of the activity of the wild-type mouse glucocorticoid receptor induced by either physiological or pharmacological doses of dexamethasone.
Kasai
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Department of Pharmacology, Kawasaki Medical School, Kurashiki City, Japan.
D000728:Androgens / Q000494:pharmacology / Q000502:physiology*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D015870:Gene Expression; D008297:Male; D008325:Mammary Neoplasms, Experimental / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D010957:Plasmids; D011965:Receptors, Glucocorticoid / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D000728:Androgens; D004273:DNA, Neoplasm; D011965:Receptors, Glucocorticoid