Selection of deletion mutants by polymerase chain reaction.
Polymerase chain reaction (PCR) based DNA amplification has replaced many time-consuming protocols in molecular biology. Here we describe a simple strategy to quickly select deletion mutants based on PCR methodology which then can be confirmed by nucleotide sequencing. A forward PCR primer is designed in such a way to recognize only the wild type sequences in the amplification reaction and thus a negative selection identifies the deletion in the samples.
Atreya
C D
CD
Department of Plant Pathology, University of Kentucky, Lexington 40546.
Atreya
P L
PL
Pirone
T P
TP
D001483:Base Sequence; D002872:Chromosome Deletion; D004247:DNA / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000737:chemistry; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction
D004277:DNA, Single-Stranded; D004247:DNA