The platelet-derived growth factor isomers, PDGF-AA, PDGF-AB and PDGF-BB, induce contraction of vascular smooth muscle cells by different intracellular mechanisms.
The effect of human recombinant platelet-derived growth factor (PDGF) isoforms, (r)PDGF-AA, PDGF-AB and PDGF-BB, on contractility of rat aortic rings as well as on intracellular free Ca2+ ([Ca2+]i), intracellular pHi (pHi) and thromboxane A2 (TXA2) formation in cultured vascular smooth muscle cells (VSMC) was examined. PDGF-BB behaved similar to PDGF-AB and both have features characteristic of conventional vasoconstrictor-agonists that directly increase [Ca2+]i, activate the Na+/H+ exchanger, stimulate the TXA2 formation, and induced contraction in VSMC whereas PDGF-AA induced contraction without increasing of [Ca2+]i, pHi, and TXA2 formation.
Sachinidis
A
A
Department of Internal Medicine, University Hospital, Zürich, Switzerland.
Locher
R
R
Hoppe
J
J
Vetter
W
W
D000818:Animals; D002118:Calcium / Q000502:physiology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000502:physiology*; D010982:Platelet-Derived Growth Factor / Q000145:classification / Q000494:pharmacology*; D051381:Rats; D011994:Recombinant Proteins; D013329:Structure-Activity Relationship; D013928:Thromboxane A2 / Q000378:metabolism
D010982:Platelet-Derived Growth Factor; D011994:Recombinant Proteins; D013928:Thromboxane A2; D002118:Calcium