title: Role of the CDC8 gene in the repair of single strand breaks in DNA of the yeast Saccharomyces cerevisiae.
abstract: It has been found that the repair of single strand breaks is defective in the DNA replication mutants cdc8-1 and cdc8-3 of Saccharomyces cerevisiae both in permissive (23 degrees C) and restrictive conditions (36 degrees C). In permissive conditions we observed a significant delay in single strand break repair in a diploid strain HB7 (cdc8-1/cdc8-1), as compared with the wild-type strain. Under restrictive conditions no repair was observed, but rather degradation of MMS-damaged DNA occurred. It has been also found that the repair of single strand breaks in yeast is inhibited by cycloheximide but not by hydroxyurea.
authors: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw.
authors: H
authors: H
authors: Baranowska
authors: D
authors: D
authors: Zaborowska
authors: W J
authors: WJ
authors: Jachymczyk
authors: J
authors: J
authors: Zuk
mesh_terms: D002499:Centrifugation, Density Gradient; D003513:Cycloheximide / Q000494:pharmacology; D004260:DNA Repair / Q000187:drug effects; D004271:DNA, Fungal / Q000187:drug effects; D004171:Diploidy; D005800:Genes, Fungal; D006918:Hydroxyurea / Q000494:pharmacology; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013696:Temperature
chemical_list: D004271:DNA, Fungal; D003513:Cycloheximide; D008741:Methyl Methanesulfonate; D006918:Hydroxyurea