[Potentiating effect of L-tetrahydropalmatine on the suppression of cortical tooth-pulp evoked potentials by electroacupuncture in rabbits].
Cortical potentials evoked by stimulation of the contralateral tooth-pulp were recorded epidurally from the SI cortex of rabbits anesthetized with urethane and chloralose. It was found that nociceptive components of the evoked potential consisted of P1 and P2 wavelets with a relative stable peak latency of 22.5 +/- 1.2 ms and 66.1 +/- 1.9 ms respectively. Higher intensity of tooth pulp stimulation was required for appearance of P2 than P1. Diazepam, a non-analgesic sedative, reduced P1 but not P2 amplitude. On the contrary, dolantin, an analgesic, suppressed P2 but showed no significant influence on P1. The results suggest that P2, but not P1 might be related to pain. The effects of l-tetrahydropalmatine (1-THP) and electroacupuncture on P2 were observed on 12 animals. The results showed that both iv l-THP 8mg/kg and electroacupuncture brought forth a decrease in P2 amplitude by 40.3 +/- 14% and 59.3 +/- 10% respectively, while electroacupuncture combined with l-THP produce a further decrease in P2 amplitude by 92.8 +/- 7%. Furthermore, the inhibitory periods of P2 amplitude were significantly prolonged after electroacupuncture combined with l-THP. The results indicated that l-THP enhanced the suppression of P2 by electroacupuncture.
Wu
G
G
Department of Neurobiology, Shanghai Medical University.
Jiang
J W
JW
Wu
G C
GC
Cao
X D
XD
D015667:Acupuncture Analgesia; D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D001600:Berberine Alkaloids / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000502:physiology*; D003782:Dental Pulp / Q000502:physiology; D004558:Electric Stimulation; D015671:Electroacupuncture; D005071:Evoked Potentials / Q000187:drug effects; D005260:Female; D008297:Male; D010146:Pain / Q000503:physiopathology; D011817:Rabbits; D013237:Stereoisomerism
D000470:Alkaloids; D001600:Berberine Alkaloids; C014215:tetrahydropalmatine