A rapid and specific high-performance liquid chromatographic assay for theophylline in biological fluids.
A rapid, specific high-performance liquid chromatographic analysis of theophylline in plasma, serum, and saliva is described. Proteins present in the biological samples are precipitated with 6% perchloric acid and the clear supernatant is chromatographed on a reversed-phase column. Only 100 microL of serum is required and concentrations as low as 0.07 micrograms/mL can be measured accurately. Other xanthines do not interfere in the assay. Within- and between-day variation is less than or equal to 2.2%. The method shows less bias and greater precision than the TDx (Abbott Diagnostics) procedure commonly used in clinical laboratories.
Blanchard
J
J
Department of Pharmaceutical Sciences, College of Pharmacy, University of Arizona, Tucson 85721.
Harvey
S
S
Morgan
W J
WJ
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D012463:Saliva / Q000737:chemistry*; D013806:Theophylline / Q000032:analysis / Q000097:blood*
D013806:Theophylline