Purification of N-terminal hexapeptide of big gastrin from human urine.
We previously demonstrated that extremely high amounts of N-terminal big gastrin (G-34) fragments are excreted in human urine and three of them are N-terminal octa-, nona-, and decapeptide of G-34. Our subsequent examination revealed that there exists a considerable amount of another N-terminal G-34 fragment in urine, less hydrophobic than the three peptides. We purified this fragment from urine of an achlorhydric patient and determined the structure: less than Glu-Leu-Gly-Pro-Gln-Gly. The purification was carried out by Sep-Pak C18 cartridges, Sephadex G-25, and reverse phase HPLC. The structure was determined by a combination of amino acid analysis, amino acid sequence analysis, and mass spectral analysis. N-terminal hexapeptide of G-34 is the second richest component of urinary N-terminal G-34 fragments next to N-terminal octapeptide of G-34 in normal subjects.
Higashimoto
Y
Y
Second Department of Internal Medicine, Osaka University Medical School, Japan.
Shinomura
Y
Y
Nagao
K
K
Yasunaga
Y
Y
Tamura
T
T
Tarui
S
S
D000126:Achlorhydria / Q000652:urine; D000595:Amino Acid Sequence; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005755:Gastrins / Q000652:urine*; D006801:Humans; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000652:urine*; D012995:Solubility
D005755:Gastrins; D011498:Protein Precursors; C013706:big gastrin