Antibody organization on lipid films. Influence of pH and interchain disulphide reduction.
Two distinct lattice structures are observed for two-dimensional (2-D) antibody organization on phospholipid films. A low-order, small-unit-cell, square lattice is obtained at pH 7 and below for mouse IgE, mouse IgG2a and IgG2b and rabbit IgG. At pH 7.5 and above, the observed lattice structure switches to a large-unit-cell, hexagonal type for rabbit IgG and mouse IgE. Interchain disulphide reduction by exposure to 2 mM-dithiothreitol results in the formation of the compact 2-D lattice for all cases and for all pH conditions.
Uzgiris
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GE Corporate Research and Development Center, Schenectady, NY 12301.
D000818:Animals; D003460:Crystallization; D004220:Disulfides; D004229:Dithiothreitol / Q000494:pharmacology; D006241:Haptens; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000737:chemistry / Q000648:ultrastructure*; D008051:Lipid Bilayers; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D011817:Rabbits / Q000276:immunology
D004220:Disulfides; D006241:Haptens; D007074:Immunoglobulin G; D008051:Lipid Bilayers; D046911:Macromolecular Substances; D004229:Dithiothreitol