Immuno-cross-reactivity of histidine and dopa decarboxylases.
Immuno-cross-reactivity between histidine decarboxylase (HDC) and dopa decarboxylase (DDC) was investigated. By comparing the cDNA sequences of rat HDC with rat and guinea-pig DDCs, we found a region that may possibly be related to the cross-reactivity of anti-rat HDC antibody with guinea-pig DDC. The peptide encoded by this region was synthesized and anti-peptide antibody was prepared. We also purified HDC and DDC homogeniously from fetal rat liver and guinea-pig liver, respectively. On immunoblotting, anti-peptide antibody recognized both rat HDC and guinea-pig DDC. Anti-HDC polyclonal antibody which also recognizes both enzymes detected only rat HDC when it was absorbed by the peptide. This result indicates that this region is responsible for the immuno-cross-reactivity of anti-rat HDC antibody with guinea-pig DDC.
Mizuguchi
H
H
Department of Pharmacology II, Osaka University Faculty of Medicine, Japan.
Yabumoto
M
M
Imamura
I
I
Fukui
H
H
Wada
H
H
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D003429:Cross Reactions; D004247:DNA / Q000737:chemistry; D004296:Dopa Decarboxylase / Q000235:genetics / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D006640:Histidine Decarboxylase / Q000235:genetics / Q000276:immunology; D015151:Immunoblotting; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D051381:Rats; D011919:Rats, Inbred Strains
D000906:Antibodies; D004247:DNA; D004296:Dopa Decarboxylase; D006640:Histidine Decarboxylase