Relaxin-like peptide in ascidians. II. Bioassay and immunolocalization with anti-porcine relaxin in three species.
A substance related to vertebrate relaxin, previously identified by radioimmunoassay and Northern hybridization in the ascidian Herdmania momus, was also purified and tested in bioassay in another species, Ciona intestinalis. In addition, immunocytochemistry with anti-porcine relaxin was performed, at the light and electron microscopic levels, on sections of ovary from three different species, living in various environmental conditions. A positive immunoreaction was located specifically in follicle cells surrounding mature oocytes. The role of this relaxin-like substance in ascidian reproduction is unknown.
Georges
D
D
Laboratoire de Biologie Cellulaire Animale, Université Joseph Fourier, Grenoble, France.
Viguier-Martinez
M C
MC
Poirier
J C
JC
D000818:Animals; D001681:Biological Assay; D002850:Chromatography, Gel; D005260:Female; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D010053:Ovary / Q000378:metabolism*; D012065:Relaxin / Q000096:biosynthesis* / Q000302:isolation & purification; D014561:Urochordata / Q000378:metabolism*
D012065:Relaxin