Mannosidase II and the 135-kDa Golgi-specific antigen recognized monoclonal antibody 53FC3 are the same dimeric protein.
Monoclonal antibodies are frequently used as organelle-specific markers without identifying the specific antigen recognized. We have purified the protein recognized by a Golgi-specific monoclonal antibody 53FC3 (Burke, B., Griffiths, G., Reggio, H., Louvard, D., and Warren, G. (1982) EMBO J. 1, 1621-1628). Peptide microsequencing suggested that this antigen is mannosidase II, which was confirmed by cross-immunoprecipitation with an anti-mannosidase II antibody and by precipitation of mannosidase activity with the monoclonal antibody. Mannosidase II was found to exist normally as a disulfide-linked dimer.
Baron
M D
MD
Department of Molecular Biology, Karolinska Institute, Novum Research Centre, Huddinge, Sweden.
Garoff
H
H
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000941:Antigens / Q000276:immunology*; D003001:Cloning, Molecular; D004220:Disulfides; D006056:Golgi Apparatus / Q000276:immunology*; D007163:Immunosorbent Techniques; D046911:Macromolecular Substances; D008361:Mannosidases / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats; D014357:Trypsin / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D000941:Antigens; D004220:Disulfides; D046911:Macromolecular Substances; D011092:Polyethylene Glycols; D017830:Octoxynol; D008361:Mannosidases; C038829:mannosyl-oligosaccharide 1,3 - 1,6-alpha-mannosidase; D014357:Trypsin