Role of fatty acid in the control of protein synthesis in liver cells.
Concentrations of oleate (0.2-1 mM) within the physiological range of plasma free fatty acids induced a dose dependent statistically significant inhibition of protein labelling in isolated liver cells. The inhibitory effect was as high as 50% and it was not impeded when long chain fatty acid oxidation was prevented. Experiments carried out with hepatocytes from 48 h fasted rats, incubated in the absence of any exogenous energy source, show that the inhibition of endogenous long chain fatty acid oxidation induced a decreased rate of protein synthesis apparently related to changes in the cellular energy state. It is concluded that fatty acids play a dual role in the regulation of protein synthesis in liver cells: 1. endogenous fatty acids appear to be the main energy fuel for protein synthesis when no other exogenous substrate is present and the carbohydrate stores are low; 2. exogenous fatty acids seem to control protein synthesis by interacting with some key regulatory step.
González-Manchón
C
C
Endocrine Physiology Unit, Centro de Investigaciones Biológicas, Madrid, Spain.
Parrilla
R
R
Ayuso
M S
MS
D000818:Animals; D004852:Epoxy Compounds / Q000494:pharmacology*; D005227:Fatty Acids / Q000494:pharmacology*; D005943:Gluconeogenesis; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D009829:Oleic Acids / Q000378:metabolism / Q000494:pharmacology*; D010084:Oxidation-Reduction; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains
D004852:Epoxy Compounds; D005227:Fatty Acids; D009829:Oleic Acids; C021756:2-tetradecylglycidic acid