Repair of radiation induced damage in two human tumour cell lines grown as spheroids and monolayers.
Growth curves of two human tumour cell lines grown as multicellular tumour spheroid (MTS) were used to obtain survival estimates by back-extrapolation after split and single dose irradiation. Neuroblastoma (NB-100) and squamous cell carcinoma (HN-1) single cells from monolayer culture were assessed for repair of sublethal and potentially lethal damage. The extent of repair was calculated on an iso-effect basis. When grown as spheroids squamous cell carcinoma cells showed a higher capacity to repair sublethal damage than neuroblastoma cells. Repair of potentially lethal damage did not contribute to this higher capacity of HN-1 cells, since this cell line was found to be deficient for this type of repair. Using the recovery ratio to estimate the beta-component of the survival curves, it was found that differences in repair capacity were determined by the alpha-component of the equation. Our results show the feasibility of back-extrapolating multicellular tumour spheroid growth curves to obtain survival estimates that can be applied to establish sublethal damage repair capacity.
Schwachöfer
J H
JH
Department of Radiotherapy, University Hospital, Nijmegen, The Netherlands.
Crooijmans
R P
RP
Borm
G F
GF
van Gasteren
J J
JJ
Hoogenhout
J
J
Kal
H B
HB
D002294:Carcinoma, Squamous Cell / Q000473:pathology; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D004249:DNA Damage / Q000528:radiation effects*; D004260:DNA Repair / Q000528:radiation effects*; D004273:DNA, Neoplasm / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D009447:Neuroblastoma / Q000473:pathology; D011832:Radiation Injuries / Q000473:pathology*; D011836:Radiation Tolerance; D014062:Tongue Neoplasms / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000528:radiation effects
D004273:DNA, Neoplasm