Inhibition by L-glutamine of the release of endothelium-derived relaxing factor from cultured endothelial cells.
L-Glutamine (0.02-2 mM) but not D-glutamine (0.2 mM and 2 mM) inhibited the release of endothelium-derived relaxing factor (EDRF) from bovine aortic endothelial cells cultured in the presence or absence of L-arginine. Inhibition was maximal at a concentration of 200 microns, and was reversed by L-arginine (50 microns) but not D-arginine (100 microns). L-Glutamic acid (2 mM) or ammonium chloride (1 mM), putative products of the metabolism of L-glutamine in endothelial cells, had no effect on EDRF release. L-Glutamine (0.2 mM and 2 mM) but not D-glutamine (2 mM), L-glutamic acid (2 mM) or ammonium chloride (1 mM) also inhibited the generation of L-arginine in endothelial cells. Thus, L-glutamine inhibits EDRF release by preventing the generation of L-arginine.
Hecker
M
M
William Harvey Research Institute, St. Bartholomew's Hospital Medical College, London.
Mitchell
J A
JA
Swierkosz
T A
TA
Sessa
W C
WC
Vane
J R
JR
D000818:Animals; D001120:Arginine / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005973:Glutamine / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism*; D013237:Stereoisomerism
D005973:Glutamine; D009569:Nitric Oxide; D001120:Arginine