[Pharmacological studies on puerariae flos. II. The effects of puerariae flos on alcohol-induced unusual metabolism and experimental liver injury in mice].
The present study was designed to examine the effects of methanolic extract (PE-ME), isoflavonoid fraction (PF-IF), triterpenoid saponin fraction (PF-SP) and N-acyl-N1-glucosyl-tryptophan (PF-P) isolated from puerariae flos on alcohol-induced unusual metabolism (as for glucose (BG), triglyceride (TG), and urea nitrogen (BUN) level in blood) and experimental liver injury (model: CCl4- and high fatty food induced) in mice. These alcohol-induced increasing responses were inhibited by the extracted and refined substances from puerariae flos. In short, PF-ME (4500 mg/kg) and PF-P (400 mg/kg) inhibited an increase in BG level induced by alcohol, whereas PF-IF (1000 mg/kg) and PF-SP (1000 mg/kg) did not. Similary, PF-ME and PF-SP inhibited an increase in TG induced by alcohol, whereas PF-IF did not. In addition, PF-IF and PF-SP inhibited increasing BUN level. Still more, PF-IF and PF-SP significantly inhibited an increase in gulutamate oxalacetate transaminase or gulutamate pyruvate transaminase level induced by high-fatty food and CCl4 in control animals. Especially PF-IF (250 mg/kg) administration showed a remarkable effect (inhibition: 76.3%) in control animals. These results suggested that puerariae flos or its combination drugs may be a useful drug as a traditional medicinal system for counteraction to drinking.
Niiho
Y
Y
Research Department of pharmacology & Analytical chemistry, Ohta's Isan Co. Ltd., Tokyo, Japan.
Yamazaki
T
T
Nakajima
Y
Y
Itoh
H
H
Takeshita
T
T
Kinjo
J
J
Nohara
T
T
D000818:Animals; D001786:Blood Glucose; D001806:Blood Urea Nitrogen; D002252:Carbon Tetrachloride Poisoning / Q000188:drug therapy / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D000431:Ethanol / Q000037:antagonists & inhibitors*; D005234:Fatty Liver / Q000188:drug therapy / Q000378:metabolism; D005260:Female; D051379:Mice; D014280:Triglycerides / Q000097:blood
D001786:Blood Glucose; D004365:Drugs, Chinese Herbal; D014280:Triglycerides; D000431:Ethanol