Comparison of conidial and mycelial allergens of Alternaria alternata.
Alternaria allergens, separated by SDS polyacrylamide gel electrophoresis and transferred onto a nitrocellulose membrane, were identified using sera from Alternaria-allergic patients. 30 Alternaria components of spore and mycelium, ranging in molecular weight from more than 150 to 12 kD, bound IgE antibodies. 15 were detected by more than 25% of the sera and only 4 (85, 56, 42, 31 kD) by more than 50% of the sera. Among these four major allergens, the 56-kD component was present mostly in the spore extract, the 85- and 42-kD fractions in the mycelium extract and the 31-kD in both extracts. The 31-kD component showed the highest IgE binding and the highest frequency of binding (95% with mycelium extract and 79% with spore extract). It was composed of two subunits.
Paris
S
S
Unité de Mycologie, Institut Pasteur, Paris, France.
Fitting
C
C
Latgé
J P
JP
Herman
D
D
Guinnepain
M T
MT
David
B
B
D000485:Allergens / Q000032:analysis* / Q000276:immunology; D000528:Alternaria / Q000276:immunology*; D000818:Animals; D015153:Blotting, Western; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D007073:Immunoglobulin E / Q000276:immunology; D008970:Molecular Weight; D011817:Rabbits
D000485:Allergens; D007073:Immunoglobulin E