Correct splicing of modified introns of a Rhizopus proteinase gene in Saccharomyces cerevisiae.
The intron of the Rhizopus aspartic proteinase gene (RNAP-I) was modified by in vitro mutagenesis and examined for its splicing efficiency in Saccharomyces cerevisiae. The wild-type intron of the RNAP-I gene was not spliced at all in spite of its structural similarity to introns of S. cerevisiae. The primary transcript of the RNAP-I gene was converted to correctly translatable mRNA only when the complete consensus sequence of S. cerevisiae introns (i.e. 5'-GTATGT-----TACTAAC-----TAG-3') was introduced into its intron, although the efficiency of splicing was low. It is also shown that transformants carrying the RNAP-I gene with the complete consensus sequence of S. cerevisiae introns produce active RNAP-I protein.
Ashikari
T
T
Institute for Fundamental Research Center, Suntory L.T.D., Osaka, Japan.
Amachi
T
T
Yoshizumi
H
H
Horiuchi
H
H
Takagi
M
M
Yano
K
K
D016282:Aspartic Acid Endopeptidases / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D016384:Consensus Sequence; D005800:Genes, Fungal; D007438:Introns; D008969:Molecular Sequence Data; D016296:Mutagenesis; D014176:Protein Biosynthesis; D012326:RNA Splicing; D012233:Rhizopus / Q000201:enzymology / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic
D016282:Aspartic Acid Endopeptidases