The technique of polymerase chain reaction--a new diagnostic tool in microbiology and other scientific fields (review).
The polymerase chain reaction, a method of so far unknown sensitivity and specificity, is about to become an important diagnostic tool in microbiology. Practically even a single bacterium, virus particle, or parasite can be detected by it. Furthermore, this technique has been used with highly promising results in other scientific fields like genetics, forensic medicine and archeology. This article reviews technical aspects and variations of this new technique.
Thiele
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Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität, Giessen.
D000818:Animals; D001424:Bacterial Infections / Q000175:diagnosis*; D004247:DNA / Q000032:analysis*; D005554:Forensic Medicine / Q000379:methods; D005818:Genetic Engineering / Q000379:methods; D006801:Humans; D010272:Parasitic Diseases / Q000175:diagnosis*; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D014777:Virus Diseases / Q000175:diagnosis*
D004247:DNA