Effects of low protein diet in puromycin aminonucleoside-induced nephrotic rats.
Wistar rats were injected with puromycin aminonucleoside (PAN) to induce massive proteinuria and were raised on a low protein diet to determine whether the urinary protein excretion might be reduced. Although PAN nephrotic rats fed with a normal protein diet containing 20% protein (group A) did not show any reduction of their urinary protein excretion, PAN rats fed with a low protein diet containing 6% protein (group B) revealed a marked reduction at day 9 of the present study in comparison with group A. The total serum protein levels were low in groups A and B as compared to group C, the non-treated control. However, group A recovered to normal levels at day 13. In terms of the absolute amount of oral protein intake, marked differences were observed between groups B and C, but little difference was observed between groups A and C. The body weight in all three groups was decreased at 8 days after the start of this study. The serum levels of creatinine were elevated to 1.1 +/- 0.3 mg/dl and 1.0 +/- 0.2 mg/dl in groups A and B, respectively, on day 7, but abruptly recovered to normal levels by day 12. These data indicate that the decreased urinary protein excretion in group B might be dependent on the total protein volume intake, and not on the renal function nor serum levels of total protein.
Shibasaki
T
T
Second Department of Internal Medicine, Jikei University School of Medicine.
Gomi
H
H
Ishimoto
F
F
Sakai
O
O
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D004044:Dietary Proteins / Q000008:administration & dosage*; D007668:Kidney / Q000503:physiopathology; D008297:Male; D009404:Nephrotic Syndrome / Q000139:chemically induced* / Q000378:metabolism / Q000503:physiopathology; D011507:Proteinuria / Q000517:prevention & control; D011692:Puromycin Aminonucleoside / Q000009:adverse effects*; D051381:Rats; D011919:Rats, Inbred Strains
D001798:Blood Proteins; D004044:Dietary Proteins; D011692:Puromycin Aminonucleoside