[Mutagenesis of vitamin B2 producer by using protoplasts].
Studies on preparation, regeneration and ultraviolet-mutagenesis of protoplasts of vitamin B2 producer Eremothecium aohbyii were reported. By using a complex enzyme system (0.5% snial digestase + 0.5% cellulase), a great number of protoplasts were obtained. Ultraviolet-mutagenesising positive mutation ratio is 14.29%. The HPLC analysis indicated that a lot of mutants were screened by using ultraviolet-mutagenesis mutation of protoplasts, Vitamin B2 produced by the mutant E3 increased 116.4%.
Li
Q
Q
Department of Biology, Hebei University, Baoding.
Zhu
B
B
Li
L
L
An
H
H
Wang
J
J
D009154:Mutation / Q000528:radiation effects*; D011523:Protoplasts / Q000528:radiation effects; D012256:Riboflavin / Q000096:biosynthesis; D004718:Saccharomycetales / Q000235:genetics* / Q000378:metabolism; D014466:Ultraviolet Rays
D012256:Riboflavin