[The partial characterization of neutrophils and lymphocytes chemotactic factors from murine anagenic hair bulb extract].
The murine anagenic ++ hair bulb extract (HBE) showed a chemotactic activity for both neutrophils and lymphocytes in vitro. The activity for both cells was retained after heating at 56 degrees C for 30 min, and dialysis. However, heating at 100 degrees C for 10 min inactivated the chemotactic activity for neutrophils, but not for lymphocytes. Activity for neutrophils was sensitive to degradation by trypsin or pronase but stable to neuraminidase treatment. On the other hand, activity for lymphocytes was not affected by protease treatment, but was reduced by approximately 30% after being treated by neuraminidase. These findings suggest that HBE contains at least two kinds of chemoattractants for neutrophils and lymphocytes.
Song
Y
Y
Department of Dermatology, Juntendo University School of Medicine.
Miyazawa
Y
Y
Takamori
K
K
D000818:Animals; D019744:Chemokines, C; D004355:Drug Stability; D006197:Hair / Q000737:chemistry*; D006358:Hot Temperature; D008222:Lymphokines / Q000302:isolation & purification* / Q000502:physiology; D008297:Male; D051379:Mice; D012795:Sialoglycoproteins / Q000302:isolation & purification* / Q000502:physiology; D014020:Tissue Extracts / Q000032:analysis*
D019744:Chemokines, C; D008222:Lymphokines; D012795:Sialoglycoproteins; D014020:Tissue Extracts; C486249:Xcl1 protein, mouse; C018997:lymphotactin