Effect of gossypol on trypomastigotes and amastigotes of Trypanosoma cruzi.
Bloodstream Trypanosoma cruzi trypomastigotes isolated from infected mice undergo reduction of motility and structural damages after 5 to 45 min exposure to gossypol at concentrations ranging from 5 to 50 microM. When 1% serum albumin is added to the incubation medium, no alterations of parasites are observed, even with 100 microM gossypol. Intracellular T. cruzi amastigotes in infected Vero cell cultures exposed to 5 microM gossypol for 2 h do not show changes. Incubation with 5 microM gossypol for 48 h produces complete disruption of host cells; however, the amastigotes they contain show only minor alterations. The observations indicate that, in protein-rich media, gossypol is complexed into associations which have no activity on the different forms of the T. cruzi biological cycle.
Rovai
L E
LE
Cátedra de Química Biológica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Argentina.
Aoki
A
A
Gerez de Burgos
N M
NM
Blanco
A
A
D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D006072:Gossypol / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D014344:Trypanocidal Agents / Q000494:pharmacology; D014349:Trypanosoma cruzi / Q000187:drug effects* / Q000648:ultrastructure
D014344:Trypanocidal Agents; D006072:Gossypol