Capillary isotachophoresis with UV and tandem mass spectrometric detection for peptides and proteins.
The application of capillary isotachophoresis (CITP) and combined CITP-mass spectrometry (MS) for peptides and proteins is demonstrated. Separation of simple peptide mixtures, as well as enzymatic digets of proteins, is also reported using CITP with UV detection. The potential utility of CITP for proteins is demonstrated. Initial studies of combined CITP-MS of enzymatic digests is also demonstrated, showing the potential for rapid sequence determination.
Smith
R D
RD
Chemical Sciences Department, Pacific Northwest Laboratory Richland, Washington 99352.
Fields
S M
SM
Loo
J A
JA
Barinaga
C J
CJ
Udseth
H R
HR
Edmonts
C G
CG
D000595:Amino Acid Sequence; D004586:Electrophoresis / Q000379:methods*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D010455:Peptides; D011506:Proteins