title: Revision of the validity of CAMP tests for Listeria identification. Proposal of an alternative method for the determination of haemolytic activity by Listeria strains.
abstract: The validity of CAMP tests with Staphylococcus aureus and Rhodococcus (Corynebacterium) equi as defined for Listeria identification was revised. This characterization method appeared to be unreliable for two reasons: first, a positive CAMP test with R. equi is not specific for Listeria ivanovii as Listeria monocytogenes (and Listeria seeligeri) give also a clear positive reaction; second, doubtful reactions could be observed with S. aureus when assaying haemolytic and non-haemolytic Listeria strains (possibility of false negative and false positive results; subjectivity of the interpretation). The use of a Microplate technique previously described instead of CAMP tests is proposed for the reliable demonstration of the haemolytic character of Listeria in the routine identification of these organisms.
authors: Department of Animal Pathology I, Veterinary Faculty, University of León, Spain.
authors: J A
authors: JA
authors: Vazquez-Boland
authors: L
authors: L
authors: Dominguez
authors: J F
authors: JF
authors: Fernandez
authors: E F
authors: EF
authors: Rodriguez-Ferri
authors: V
authors: V
authors: Briones
authors: M
authors: M
authors: Blanco
authors: G
authors: G
authors: Suarez
mesh_terms: D015373:Bacterial Typing Techniques; D003951:Diagnostic Errors; D005069:Evaluation Studies as Topic; D005516:Food Microbiology; D006461:Hemolysis; D008087:Listeria / Q000145:classification*; D012240:Rhodococcus; D013211:Staphylococcus aureus