Direct resolution of some phenylglycines by liquid chromatography on a chiral crown ether phase.
The optical resolution of a series of 12 amino acids of the phenylglycine family was studied using a chiral crown ether column. The effects of pH, temperature, and mobile phase additive were investigated. In three examples it was confirmed that the R enantiomer eluted prior to the S enantiomer. Most phenylglycines were resolved with large separation factors; those with two functional groups substituents on opposing sides of the phenyl ring, however, were not well separated.
Udvarhelyi
P M
PM
Department of Pharmacology, School of Medical Sciences, University of Bristol, United Kingdom.
Watkins
J C
JC
D002853:Chromatography, Liquid; D005998:Glycine / Q000031:analogs & derivatives*; D006863:Hydrogen-Ion Concentration; D000432:Methanol; D010649:Phenylalanine; D013696:Temperature
D010649:Phenylalanine; D005998:Glycine; D000432:Methanol