Real-time imaging of intracellular calcium change with simultaneous single channel recording in mammary epithelial cells.
A method of continuous image subtraction of fura-2 fluorescence made it possible to observe real-time (video rate) changes in intracellular calcium (Cai). This simple method is very useful for simultaneous measurements in electrophysiology with a system containing cells of different states. A method for synchronous recording of Cai change and single channel activity is also described. In cultured mammary epithelial cells, these methods revealed a propagating Cai signal induced by mechanical stimulation and spontaneous Cai oscillation with synchronous activation of calcium-activated potassium channels.
Furuya
K
K
Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki, Japan.
Enomoto
K
K
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D016257:Fura-2; D007091:Image Processing, Computer-Assisted; D008321:Mammary Glands, Animal / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D008856:Microscopy, Fluorescence; D014741:Video Recording
D002118:Calcium; D016257:Fura-2