title: Imaging in scanning microscopes with slit-shaped detectors.
abstract: We consider the imaging in scanning microscopes employing point and slit-shaped detectors in both the bright-field and fluorescent mode. In particular we consider the three-dimensional aspects of the imaging and show inter alia that acceptable, albeit asymmetrical, images result from a slit detector system at low levels of defocus. The situation becomes worse as the defocus is increased although acceptable extended-focus and isometric images are still possible in some cases.
authors.affiliation: University of Oxford, U.K.
authors.forename: T
authors.initials: T
authors.lastname: Wilson
authors.forename: S J
authors.initials: SJ
authors.lastname: Hewlett
mesh_terms: D007091:Image Processing, Computer-Assisted; D008433:Mathematics; D008853:Microscopy / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*