title: Native bromoperoxidases do not bind to nitrocellulose: use of DEAE-cellulose as an alternative in blotting.
abstract: Bromoperoxidases were investigated by protein blotting after polyacrylamide gel electrophoresis. While the denatured proteins bound to nitrocellulose, the native enzymes did not. Instead, they could be transferred successfully to DEAE-cellulose. Procedures for immunostaining and glycoprotein detection with concanavalin A on DEAE-cellulose are described. The results indicate that binding of native proteins to nitrocellulose can not necessarily be assumed. DEAE-cellulose is suitable both to investigate this phenomenon or as a substitute for nitrocellulose in blotting of native proteins.
authors.affiliation: Institut für Pharmazeutische Biologie, Universität Bonn, Federal Republic of Germany.
authors.forename: P
authors.initials: P
authors.lastname: Jordan
authors.forename: H
authors.initials: H
authors.lastname: Vilter
mesh_terms: D015153:Blotting, Western; D003101:Collodion; D003208:Concanavalin A; D003636:DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis; D010544:Peroxidases / Q000032:analysis*; D000459:Phaeophyceae / Q000201:enzymology
chemical_list: D006023:Glycoproteins; D003208:Concanavalin A; D003101:Collodion; D003636:DEAE-Cellulose; D010544:Peroxidases; C026195:bromide peroxidase