title: Cortical radial glia: identification in tissue culture and evidence for their transformation to astrocytes.
abstract: Radial glia are transiently present in the developing cerebral cortex, where they are thought to guide the migration of neurons from the proliferative zone to the forming cortical plate. To provide a framework for experimental studies of radial glia, we have defined morphological and immunocytochemical criteria to identify them in primary cultures of cortical cells obtained at embryonic day 13 in the mouse. Cortical radial glia in culture for 1-2 d resemble radial glia in vivo: they have a long, thin, unbranched process extending from one or both ends of the elongated cell body and are labeled with the monoclonal antibody RC1 but not with antibodies to glial fibrillary acidic protein (abGFAP). We tested the specificity of RC1 by double-labeling with a panel of cell-type specific antibodies, and found that it labels radial glia, astrocytes, and fibroblast-like cells, but not neurons. Fibroblasts are easily distinguished from glia by morphology and by labeling with antibodies to fibronectin. To test the hypothesis that radial glia become astrocytes when their developmental role is complete, we examined their morphological and immunocytochemical development in culture. After 3-4 d in vitro radial glia develop several branched processes; in this transitional stage they are labeled by both RC1 and abGFAP. Many radial glia lose RC1 immunoreactivity as they become increasingly branched and immunoreactive to abGFAP. In areas of the cultures that have few neurons and in cultures depleted of neurons by washing, flat, nonprocess-bearing glia predominate. These cells do not lose immunoreactivity to RC1 during the 9-d period of observation even though they acquire GFAP.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Cell Biology, Washington University School of Medicine, St. Louis, Missouri 63110.
authors.forename: S M
authors.initials: SM
authors.lastname: Culican
authors.forename: N L
authors.initials: NL
authors.lastname: Baumrind
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
authors.forename: A L
authors.initials: AL
authors.lastname: Pearlman
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D001253:Astrocytes / Q000502:physiology*; D002450:Cell Communication; D002460:Cell Line; D002540:Cerebral Cortex / Q000166:cytology*; D046508:Culture Techniques; D007150:Immunohistochemistry; D051379:Mice / Q000196:embryology; D009457:Neuroglia / Q000166:cytology* / Q000502:physiology; D009474:Neurons / Q000502:physiology
chemical_list: D000911:Antibodies, Monoclonal