title: Brain isozyme of glycogen phosphorylase: immunohistological localization within the central nervous system.
abstract: An antibody specific for the predicted carboxyterminal sequence of the human brain isozyme of glycogen phosphorylase (alpha-1,4-D-glucan:orthophosphate D-glucosyltransferase, EC 2.4.1.1) was generated to verify the carboxyterminal amino acid sequence of this protein. The isozyme-specific antibody was used to examine the localization of this protein in primate and non-primate brain. The highest levels of the brain isozyme in cerebrum and cerebellum were found in fibrous astrocytes, many with glial processes that appear to terminate upon blood vessels.
authors.affiliation: Department of Neurology, University of California, Davis 95616.
authors.forename: P C
authors.initials: PC
authors.lastname: Ignacio
authors.forename: B A
authors.initials: BA
authors.lastname: Baldwin
authors.forename: V K
authors.initials: VK
authors.lastname: Vijayan
authors.forename: R C
authors.initials: RC
authors.lastname: Tait
authors.forename: F A
authors.initials: FA
authors.lastname: Gorin
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000201:enzymology; D001921:Brain / Q000201:enzymology*; D002531:Cerebellum / Q000201:enzymology; D002540:Cerebral Cortex / Q000201:enzymology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D007527:Isoenzymes / Q000032:analysis* / Q000276:immunology; D008251:Macaca; D009413:Nerve Fibers, Myelinated; D006005:Phosphorylases / Q000032:analysis* / Q000276:immunology; D051381:Rats
chemical_list: D007527:Isoenzymes; D006005:Phosphorylases