A method for improved resolution for fluorescence microscopy using plastic-embedded material subjected to resin extraction.
A protocol is given that uses NaOH, benzene, acetone and methanol to extract epoxy resins from semithin sections. Such sections appear superior to paraffin or unsectioned materials for fluorescence microscopic observations. Use of ultrarapid films (e.g., Kodak T-Max P3200) at ISO 3200 minimizes fading without use of antifading agents and without introducing unacceptable photographic grain size.
Russell
S D
SD
Department of Botany and Microbiology, University of Oklahoma, Norman 73019-0245.
D004853:Epoxy Resins; D005456:Fluorescent Dyes; D006652:Histological Techniques; D008856:Microscopy, Fluorescence / Q000379:methods*; D010784:Photomicrography / Q000379:methods; D010944:Plants / Q000033:anatomy & histology*; D011058:Pollen / Q000033:anatomy & histology
D004853:Epoxy Resins; D005456:Fluorescent Dyes