title: Interaction of chlorpromazine with hemoglobin.
abstract: Binding of chlorpromazine (CPZ), a widely used antidepressant tranquilizer, with hemoglobin has been studied by equilibrium dialysis method. r/Cf versus r plot was typically concave downwards revealing the positive cooperative nature of binding. Binding parameters, namely the affinity constant (K) and the degree of cooperativity (nH) were determined from the Hill plot. Oxygen was found to be released gradually from hemoglobin with gradual addition of CPZ, the extent of oxygen release depending on the stoichiometric ratio of CPZ: hemoglobin (D/P).
authors.affiliation: Department of Biophysics, Molecular Biology & Genetics, University of Calcutta, India.
authors.forename: M
authors.initials: M
authors.lastname: Bhattacharyya
authors.forename: U
authors.initials: U
authors.lastname: Chaudhuri
authors.forename: R K
authors.initials: RK
authors.lastname: Poddar
mesh_terms: D001665:Binding Sites; D002746:Chlorpromazine / Q000378:metabolism* / Q000494:pharmacology; D006454:Hemoglobins / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D010100:Oxygen / Q000378:metabolism
chemical_list: D006454:Hemoglobins; D010100:Oxygen; D002746:Chlorpromazine