title: Determination of the packaging capacity of bacteriophage VWB.
abstract: VWB is a temperate bacteriophage whose chromosome has cohesive ends. VWB can stably package modified chromosomes that contain insertions of up to about 4 kilobases of foreign DNA. Phage particles containing extra DNA differ from the wild type in their increased sensitivity to chelating agents. Because of these properties, VWB is a promising cloning vector for Streptomyces venezuelae.
authors.affiliation: Department of Microbiology, Rega Institute, Katholieke Universiteit Leuven, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: Anné
authors.forename: L
authors.initials: L
authors.lastname: Van Mellaert
authors.forename: H
authors.initials: H
authors.lastname: Eyssen
mesh_terms: D001435:Bacteriophages / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D015183:Restriction Mapping; D013302:Streptomyces / Q000235:genetics*; D013696:Temperature
chemical_list: D004279:DNA, Viral; D015345:Oligonucleotide Probes