Assay of protein A in Staphylococcus hyicus subsp. hyicus by ELISA and immunoelectron microscopy.
The presence and quantity of protein A in Staphylococcus hyicus subsp. hyicus isolates were examined by an enzyme-linked immunosorbent assay (ELISA) and immunoelectron microscopy. Cell-bound protein A was demonstrated in 45 (94%) of 48 isolates from diseased pigs and in 113 (86%) of 132 isolates from healthy pigs by ELISA using peroxidase-conjugated rabbit antibody, but was not found in isolates from chickens and cows. Most of these swine isolates contained about 100 to 300 ng of cell-bound protein A/ml. Extracellular protein A was not detected in any isolates from pigs, chickens or cows. In the immunoelectron microscopy assay, swine isolates were labeled with goat anti-mouse IgG conjugated to colloidal gold particles, but chicken and cow isolates were not labeled.
Takeuchi
S
S
Department of Agriculture, Fukui Prefectural College, Japan.
Kobayashi
Y
Y
Mori
Y
Y
D000818:Animals; D002417:Cattle; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D016253:Microscopy, Immunoelectron; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary*; D013205:Staphylococcal Protein A / Q000032:analysis*; D013210:Staphylococcus / Q000032:analysis*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
D013205:Staphylococcal Protein A