Morphological study of normal and tumor cells in the head and neck region using irradiated collagen gel embedding technique: morphological study using collagen gel.
Fourteen specimens of normal, benign, or malignant tumor cells from the head and neck region were subjected for culture using 0.2% of irradiated collagen gel embedding technique. Re-differentiation of glands within the gel in serum-free medium was observed. There were marked differences in the growth patterns within the gel between normal or benign and malignant cells. Four normal glands and 5 out of 6 benign tumors showed branch-like growth patterns. On the other hand, malignant tumors showed no branch-like pattern, or could not grow at all. The results showed that the collagen gel technique could be useful for the differential diagnosis of malignancy in head and neck tumors.
Suzumura
S
S
Department of Otorhinolaryngology, Nara Medical University, Kashihara, Japan.
Imai
S
S
Morimoto
J
J
Haga
S
S
Tanaka
O
O
Miyahara
H
H
Matsunaga
T
T
Iwai
Y
Y
Iwai
M
M
D002454:Cell Differentiation; D003094:Collagen; D005782:Gels; D006258:Head and Neck Neoplasms / Q000473:pathology*; D006801:Humans; D014407:Tumor Cells, Cultured
D005782:Gels; D003094:Collagen