title: Mutational analysis of the human immunodeficiency virus type 2 (HIV-2) genome in relation to HIV-1 and simian immunodeficiency virus SIV (AGM).
abstract: We constructed an infectious molecular clone of the human immunodeficiency virus type 2 (HIV-2) and generated nine frameshift mutants corresponding to nine open reading frames identified so far. Three structural (gag, pol, env) and two regulative (tat, rev) gene mutants were not infectious, whereas vif, vpx, vpr, and nef genes were dispensable for infectivity. All of the mutants except env and rev were cytopathic in CD4+ human leukemia cells. In transfection assays, the expression of HIV-2 long terminal repeat was activated by infectious clones of HIV-1, HIV-2, and simian immunodeficiency virus from African green monkey but not by the tat mutants. However, an HIV-2 tat mutant could produce small amounts of virus proteins and particles in contrast to a rev mutant, which directed no detectable synthesis of virus proteins and virions.
authors.affiliation: Department of Viral Oncology, Kyoto University, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Shibata
authors.forename: T
authors.initials: T
authors.lastname: Miura
authors.forename: M
authors.initials: M
authors.lastname: Hayami
authors.forename: K
authors.initials: K
authors.lastname: Ogawa
authors.forename: H
authors.initials: H
authors.lastname: Sakai
authors.forename: T
authors.initials: T
authors.lastname: Kiyomasu
authors.forename: A
authors.initials: A
authors.lastname: Ishimoto
authors.forename: A
authors.initials: A
authors.lastname: Adachi
mesh_terms: D015152:Blotting, Northern; D015153:Blotting, Western; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D015497:HIV-1 / Q000235:genetics*; D015498:HIV-2 / Q000235:genetics*; D006801:Humans; D007938:Leukemia; D009154:Mutation; D010957:Plasmids; D012367:RNA, Viral / Q000235:genetics; D015183:Restriction Mapping; D015302:Simian Immunodeficiency Virus / Q000235:genetics*; D015533:Transcriptional Activation; D014162:Transfection
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral; D015500:Chloramphenicol O-Acetyltransferase