Production of a novel steroid sulfate metabolite [4,4,24-trimethylcholesta-8,14,24(28)-trien-2 alpha,3 beta,11 alpha,12 beta-tetrol 12-acetate, 3-sulfate] by Fusarium species and its biological activity.
A novel steroid sulfate, 4,4,24-trimethylcholesta-8,14-24(28)-trien-2 alpha,3 beta,11 alpha,12 beta- tetrol 12-acetate, 3-sulfate, was discovered in Fusarium spp. Forty Fusarium strains belonging to F. sporotrichioides, F. chlamydosporum, E. equiseti, F. acuminatum, F. sambucinum, F. culmorum, and F. graminearum produced the steroid on white corn grits at 25 degrees C for 20 days. This steroid sulfate is one of the more abundant and easily attainable microbial steroids. At a concentration of 160 micrograms/ml, it inhibited the growth of six fungi, two gram-positive bacteria, and an alga, as well as the germination of both wheat and tomato seeds.
Burmeister
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Northern Regional Research Center, U.S. Department of Agriculture, Peoria, Illinois 61604.
Vesonder
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D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology; D002782:Cholestenes / Q000302:isolation & purification* / Q000494:pharmacology; D005670:Fusarium / Q000145:classification / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D012639:Seeds / Q000187:drug effects; D013045:Species Specificity
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D002782:Cholestenes; C067273:4,4,24-trimethylcholesta-8,14,24(28)-trien-2,3,11,12-tetrol 12-acetate-3-sulfate