Interaction of an immature seed-specific trans-acting factor with the 5' upstream region of a rice glutelin gene.
The 5' flanking region of a glutelin gene was analyzed for interactions with nuclear proteins from immature rice seed. The specific region between positions -272 and -99 was shown to interact with nuclear proteins from immature seeds, but not with those of leaves and roots. Methylation interference experiments revealed that one factor interacted with a specific sequence element between positions -130 and -120 relative to the transcriptional start site. The sequence specificity of this DNA-protein interaction was confirmed by competition experiments using synthetic oligonucleotides. By using a synthetic oligonucleotide as a probe it was also shown that the binding activity was closely correlated with the mRNA levels of this gene during seed maturation.
Takaiwa
F
F
Department of Cell Biology, National Institute of Agrobiological Resources, Ibaraki, Japan.
Oono
K
K
D001483:Base Sequence; D001667:Binding, Competitive; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D005809:Genes, Regulator; D005983:Glutens / Q000235:genetics*; D008745:Methylation; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D015345:Oligonucleotide Probes / Q000494:pharmacology; D012275:Oryza / Q000235:genetics*
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D015345:Oligonucleotide Probes; D005983:Glutens; D004247:DNA