title: Characterization of multiple mRNAs originating from the chicken progesterone receptor gene. Evidence for a specific transcript encoding form A.
abstract: The structure of the 42-kilobase (kb) long chicken progesterone receptor (cPR) gene and of all six transcripts that are detectable on Northern blots was determined. The first of 8 exons encodes the N-terminal region A/B which is highly divergent among different species and contains a constitutive transcription activation function. The DNA (DBD)- and hormone-binding domains (HBD) are assembled from 2 and 5 exons, respectively, with the individual "zinc fingers" of the DBD encoded by separate exons. In addition to the previously described 4.5-kb cPR mRNA species, alternative polyadenylation, splicing variation, and "5'-truncation" lead to the generation of 5 further mRNAs. Most importantly, this 5'-truncation produces, by an as yet unidentified mechanism, an abundant transcript which encodes form A but not form B of cPR. Lack of splicing at the exon 2 splice-donor and polyadenylation due to a signal site in the second intron generates a previously undetected 3.4-kb mRNA species. The corresponding cDNA was sequenced in its entirety and shown to encode only region A/B and the N-terminal "finger" of the DBD. Alternative polyadenylation upstream of the signal site for the 4.5-kb mRNA is responsible for the appearance of a 3.3-kb mRNA. The longest cPR mRNA (8.2 kb) originates from a transcription termination point more than 3 kb downstream of the 4.5-kb mRNA 3'-end. Finally, the primary sequence of more than 2 kb upstream sequences of the cPR gene, containing several consensus hexamer progestin/glucocorticoid receptor-binding sites (PRE/GRE and putative Sp1 binding motifs, is discussed.
authors.affiliation: Laboratoire de Génétique Moléculaire des Eucaryotes du Centre National de la Recherche Scientifique, Faculté de Médicine, Strasbourg, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Jeltsch
authors.forename: B
authors.initials: B
authors.lastname: Turcotte
authors.forename: J M
authors.initials: JM
authors.lastname: Garnier
authors.forename: T
authors.initials: T
authors.lastname: Lerouge
authors.forename: Z
authors.initials: Z
authors.lastname: Krozowski
authors.forename: H
authors.initials: H
authors.lastname: Gronemeyer
authors.forename: P
authors.initials: P
authors.lastname: Chambon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002467:Cell Nucleus / Q000378:metabolism; D002645:Chickens; D004247:DNA / Q000235:genetics; D005091:Exons; D005796:Genes; D014644:Genetic Variation; D008969:Molecular Sequence Data; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification; D011980:Receptors, Progesterone / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D011980:Receptors, Progesterone; D004247:DNA