Construction of a fast, inexpensive rapid-scanning diode-array detector and spectrometer.
A 512-element diode-array spectroscopic detection system capable of acquiring multiple spectra at a rate of 5 ms per spectrum with an effective scan rate of 102.9 kHz has been constructed. Spectra with fewer diode elements can also be acquired at scan rates up to 128 kHz. The detector utilizes a Hamamatsu silicon photodiode-array sensor that is interfaced to Hamamatsu driver/amplifier and clock generator boards and a DRA laboratories 12-bit 160-kHz analog-to-digital converter. These are standard, commercially available devices which cost approximately $3500. The system is interfaced to and controlled by an IBM XT microcomputer. Detailed descriptions of the home-built detector housing and control/interface circuitry are presented and its application to the study of the reaction of horseradish peroxidase with hydrogen peroxide is demonstrated.
Carter
T P
TP
Institute of Molecular Biophysics, Florida State University, Tallahassee 32306-3006.
Baek
H K
HK
Bonninghausen
L
L
Morris
R J
RJ
van Wart
H E
HE
D002623:Chemistry Techniques, Analytical / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D008838:Microcomputers; D012984:Software; D013053:Spectrophotometry / Q000295:instrumentation / Q000379:methods; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods