Evaluation of chemotherapy in experimental toxocarosis by determination of specific immune complexes.
Parasitism by the larval phase of Toxocara canis is a chronic process in which the larvae survive in the tissues, resulting in the constant stimulation of the immune system. As a result, the detection of specific antibodies may not reflect the active state of the parasite. We have studied the dynamics of the production of specific immune complexes by ELISA with the monoclonal antibody TC-1 in rabbits inoculated with single and multiple doses of T. canis eggs. We also compared this with the production of specific antibodies and their possible modification after treatment with mebendazole. The specific antibodies against excretory-secretory antigen were detected with peaks at 10 and 12 weeks depending on the dose and remained positive during the entire experiment (62 weeks). Treatment caused an increase in the level of detectable antibodies dropping to similar levels to the controls. Specific immune complexes were detected only in multiple doses, and were then positive during the entire experiment. From the beginning of treatment the values of immune complexes fell quickly, remaining at undetectable levels during the rest of the experiment. For this reason the detection of specific immune complexes is a valid technique for monitoring the efficiency of treatment.
Cuellar
C
C
Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
Fenoy
S
S
Aguila
C
C
Guillen
J L
JL
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis* / Q000097:blood; D000936:Antigen-Antibody Complex / Q000096:biosynthesis* / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D008463:Mebendazole / Q000627:therapeutic use*; D011817:Rabbits; D014119:Toxocara / Q000276:immunology*; D014120:Toxocariasis / Q000188:drug therapy* / Q000276:immunology
D000909:Antibodies, Helminth; D000936:Antigen-Antibody Complex; D008463:Mebendazole