Differences in activity of minoxidil and cyclosporin A on hair growth in nude and normal mice. Comparisons of in vivo and in vitro studies.
Hair growth effects of minoxidil and cyclosporin A were assessed in a series of experiments using nude mice. Systematic monitoring of coat hair showed that untreated nude mice grow extremely sparse and transient hair in cycles. This monitoring was done by photographing each animal through at least one full growth cycle and rating peak growth on a 1 to 4 scale. Topical administration of minoxidil or minoxidil sulfate did not influence this cyclic hair growth. Orally administered minoxidil also had no effect but oral cyclosporin A increased peak hair growth. None of the treatments altered the length of the hair cycle. Direct drug effects on follicles were tested in vitro using organ cultured vibrissae from both nude and normal mice. Minoxidil stimulated hair growth in follicles from normal but not nude mice. In contrast, cyclosporin A stimulated growth only in vibrissae follicles from nude but not normal animals. These studies show that minoxidil and cyclosporin A influence hair growth differentially. Cyclosporin A directly affects nude hair follicles by apparently compensating for a genetic defect inherent in nude follicles. Minoxidil does not have a similar effect. Apparently, the biochemical pathway activated by minoxidil is not a critical defect of hair growth in nude mice. We conclude that nude mice are not useful for studying minoxidil effects but they may be useful in studying pleiotropic effects of the nude gene on hair growth.
Buhl
A E
AE
Hairgrowth Research and Biostatistics, Upjohn Company, Kalamazoo, Michigan.
Waldon
D J
DJ
Miller
B F
BF
Brunden
M N
MN
D000279:Administration, Cutaneous; D000284:Administration, Oral; D000818:Animals; D003524:Cyclosporins / Q000008:administration & dosage / Q000494:pharmacology*; D006197:Hair / Q000187:drug effects* / Q000254:growth & development; D066298:In Vitro Techniques; D051379:Mice / Q000502:physiology*; D008819:Mice, Nude / Q000502:physiology*; D008914:Minoxidil / Q000008:administration & dosage / Q000494:pharmacology*; D008954:Models, Biological; D013045:Species Specificity
D003524:Cyclosporins; D008914:Minoxidil