[Effects of serum and glucose on the proliferation of different types of cells of the human embryonic pancreas in primary culture].
We have utilized primary cultures of free-floating clusters obtained from pancreata of human cadaveric fetuses. Clusters have been incubated for 10 days in RPMI-1640 medium with 3H-thymidine and various concentrations of fetal calf serum and glucose. Viability of clusters was assessed by insulin radioimmunoassay. Cell reproduction was investigated by radioautography. We have revealed 2 populations of proliferating epithelial cells in clusters: the minor population of cells of duct-like structures and the major population of other epithelial cells. Rate of accumulation of labelled cells in both populations in clusters of 17-18-week-old fetuses was significantly higher than in clusters of 23-24-week-old fetuses. Proliferation of cells of duct-like structures was regulated by serum but not by glucose, whereas proliferation of other epithelial cells depended on both serum and glucose concentrations.
Timofeev
A V
AV
Egorov
B B
BB
Chizhevskaia
V I
VI
Pozdnev
P V
PV
Kazachkov
A R
AR
D001345:Autoradiography; D001769:Blood; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D005333:Fetus; D005865:Gestational Age; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D010179:Pancreas / Q000166:cytology*; D011863:Radioimmunoassay
D003470:Culture Media; D005947:Glucose
Vliianie syvorotki i gliukozy na proliferatsiiu raznykh tipov kletok podzheludochnoĭ zhelezy émbrionov cheloveka v pervichnoĭ kul'ture.