title: Parathyroid hormone-related protein in tumor tissues obtained from patients with humoral hypercalcemia of malignancy.
abstract: We assessed the relationship between the parathyroid hormone-related protein (PTHrP) and the development of humoral hypercalcemia of malignancy (i.e., hypercalcemia due to the production by solid tumors of hypercalcemic factors) by assaying tumor extracts from hypercalcemic and normocalcemic patients with cancer for immunoreactive PTHrP contents. Immunoreactive PTHrP was demonstrated in extracts of 21 of 22 tumor tissues obtained from 22 patients with humoral hypercalcemia of malignancy. Immunoreactive PTHrP was rarely seen in tumor tissue extracts obtained from 34 normocalcemic patients with cancer and two hypercalcemic patients with cancer with severe bone metastases. Gel filtration studies of tumor extracts revealed a molecular-size heterogeneity of immunoreactive PTHrP. These radioimmunoassay data indicate a close relationship between detection of PTHrP in tumor tissues and the development of humoral hypercalcemia of malignancy.
authors.affiliation: Growth Factor Division, National Cancer Center Research Institute, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tsuchihashi
authors.forename: K
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Miyake
authors.forename: K
authors.initials: K
authors.lastname: Otsubo
authors.forename: K
authors.initials: K
authors.lastname: Nagasaki
authors.forename: S
authors.initials: S
authors.lastname: Honda
authors.forename: Y
authors.initials: Y
authors.lastname: Akiyama
authors.forename: Y
authors.initials: Y
authors.lastname: Maeda
authors.forename: M
authors.initials: M
authors.lastname: Koike
authors.forename: O
authors.initials: O
authors.lastname: Alper
mesh_terms: D000328:Adult; D000368:Aged; D002850:Chromatography, Gel; D005260:Female; D006801:Humans; D006934:Hypercalcemia / Q000209:etiology* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis* / Q000276:immunology / Q000502:physiology; D010257:Paraneoplastic Syndromes / Q000209:etiology / Q000378:metabolism; D010281:Parathyroid Hormone / Q000032:analysis*; D044162:Parathyroid Hormone-Related Protein; D011863:Radioimmunoassay
chemical_list: D009363:Neoplasm Proteins; C470121:PTHLH protein, human; D010281:Parathyroid Hormone; D044162:Parathyroid Hormone-Related Protein