Subtypes of HLA-A1 defined on the basis of CTL precursor frequencies.
Recently we have shown that limiting dilution analysis can be used to detect cytotoxic T-cell precursor frequencies directed against individual HLA class I antigens. Using the same protocol, we have been able to define two subtypes of HLA-A1, which are indistinguishable by conventional typing sera as well as by cell-mediated lympholysis. One-dimensional isoelectric focusing analysis of the variants did not show any overall charge differences. However, family studies indicated that these HLA-A1 subtypes are genetically determined and can be distinguished on the bases of T-cell precursor frequencies in HLA-A1-negative blood donors.
Zhang
L
L
Department of Immunohaematology, Leiden University Hospital, The Netherlands.
Rinke de Wit
T F
TF
Li
S G
SG
van Rood
J J
JJ
Claas
F H
FH
D001667:Binding, Competitive; D001788:Blood Grouping and Crossmatching / Q000379:methods; D003601:Cytotoxicity Tests, Immunologic; D015788:HLA-A1 Antigen / Q000145:classification* / Q000235:genetics; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D015788:HLA-A1 Antigen