Shotgun concatenation of synthetic genes: construction of concatemeric human calcitonin genes.
A new fast and reliable approach for construction of concatemeric synthetic genes is described. The method consists of shotgun polymerization of three truncated monomeric gene units using a specific linker, followed by cloning of the recombinant clones and screening them for the presence of concatemeric genes of defined length. The method is illustrated by constructing a series of tandemly repeated human calcitonin (hCT) genes.
Ivanov
I G
IG
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
D000595:Amino Acid Sequence; D001483:Base Sequence; D002116:Calcitonin / Q000235:genetics*; D003001:Cloning, Molecular; D005813:Genes, Synthetic / Q000235:genetics*; D005821:Genetic Techniques; D006801:Humans; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics
D009841:Oligonucleotides; D011994:Recombinant Proteins; D002116:Calcitonin