title: Use of polymyxin-coated polyester cloth in the enzyme immunoassay of Salmonella lipopolysaccharide antigens.
abstract: Polyester cloth coated with polymyxin B was used to capture Salmonella typhimurium lipopolysaccharide antigens which were then quantitatively or qualitatively assayed using a specific antibody-peroxidase conjugate. This simple, rapid method can be used to assay a large number of samples by employing a large sheet of the polymyxin-coated cloth onto which multiple samples can be blotted. The method is reproducible and economical, since polymyxin B is relatively inexpensive, stable and available in pure form.
authors.affiliation: Department of Biology and Institute of Biochemistry, Carleton University, Ottawa, Canada.
authors.forename: B W
authors.initials: BW
authors.lastname: Blais
authors.forename: H
authors.initials: H
authors.lastname: Yamazaki
mesh_terms: D000327:Adsorption; D007124:Immunoenzyme Techniques; D007700:Kinetics; D008070:Lipopolysaccharides / Q000032:analysis*; D011091:Polyesters; D011112:Polymyxin B; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D012486:Salmonella typhimurium / Q000302:isolation & purification*
chemical_list: D008070:Lipopolysaccharides; D011091:Polyesters; D011112:Polymyxin B