Use of polymyxin-coated polyester cloth in the enzyme immunoassay of Salmonella lipopolysaccharide antigens.
Polyester cloth coated with polymyxin B was used to capture Salmonella typhimurium lipopolysaccharide antigens which were then quantitatively or qualitatively assayed using a specific antibody-peroxidase conjugate. This simple, rapid method can be used to assay a large number of samples by employing a large sheet of the polymyxin-coated cloth onto which multiple samples can be blotted. The method is reproducible and economical, since polymyxin B is relatively inexpensive, stable and available in pure form.
Blais
B W
BW
Department of Biology and Institute of Biochemistry, Carleton University, Ottawa, Canada.
Yamazaki
H
H
D000327:Adsorption; D007124:Immunoenzyme Techniques; D007700:Kinetics; D008070:Lipopolysaccharides / Q000032:analysis*; D011091:Polyesters; D011112:Polymyxin B; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D012486:Salmonella typhimurium / Q000302:isolation & purification*
D008070:Lipopolysaccharides; D011091:Polyesters; D011112:Polymyxin B