Serotype-related antigen of Trichosporon cutaneum in the induction of summer-type hypersensitivity pneumonitis: correlation between serotype of inhalation challenge-positive antigen and that of the isolates from patients' homes.
Inhalation challenge with the culture filtrate-antigens prepared from two strains of different serotype of Trichosporon cutaneum (TIMM 1573, serotype I; TIMM 1318, serotype II) was performed on patients with summer-type hypersensitivity pneumonitis and asymptomatic seropositive family members. Of the 17 patients, 12 were strongly positive, four were mildly so, and one was negative. Interestingly, of the 16 inhalation challenge-positive patients, four reacted to both serotypes I and II, five to serotype I only, and seven to serotype II only. There was a good correlation between the serotype inhalation challenge-positive antigen and that of T. cutaneum isolated from the homes of two patients' homes. A strain of T. cutaneum demonstrating a new serotype was isolated from the homes of two patients, one of whom was negative to both serotypes I and II. Specific antibody activity, lymphocyte proliferative response, and skin reaction to the antigens were also positive, but these findings were not useful to discriminate the inhalation challenge-positive from the inhalation challenge-negative antigen. Neither of the two asymptomatic family members responded. These results indicate that inhalation challenge in patients with summer-type hypersensitivity pneumonitis is provoked by the serotype-related antigen of T. cutaneum, reflecting the sensitization of these patients in their homes.
Ando
M
M
First Department of Internal Medicine, Kumamoto University Medical School, Japan.
Sakata
T
T
Yoshida
K
K
Yamasaki
H
H
Araki
S
S
Onoue
K
K
Shinoda
T
T
D000542:Alveolitis, Extrinsic Allergic / Q000175:diagnosis* / Q000209:etiology / Q000276:immunology; D000908:Antibodies, Fungal / Q000032:analysis; D000946:Antigens, Fungal / Q000145:classification / Q000302:isolation & purification; D001985:Bronchial Provocation Tests / Q000379:methods; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D004783:Environmental Microbiology; D006798:Housing; D006801:Humans; D007564:Japan; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D003904:Mitosporic Fungi / Q000276:immunology*; D012621:Seasons; D012703:Serotyping; D012882:Skin Tests; D014250:Trichosporon / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification
D000908:Antibodies, Fungal; D000946:Antigens, Fungal