Metabolism of dicarboxylic acids in rat hepatocytes.
[carboxyl-14C]Dodecanedioic acid (DC12) is metabolized in hepatocytes at a rate about two thirds that of [1-14C]palmitate. Shorter dicarboxylates (sebacic (DC10), suberic (DC8), and adipic (DC6) acid) are formed, mainly DC6, less DC8 and only a little DC10. In hepatocytes from clofibrate-treated rats, more polar products account for most of the breakdown products, presumably because the beta-oxidation proceeds all the way to succinate and acetyl-CoA. [carboxyl-14C]Suberic acid (DC8) is oxidized at a rate only one fifth that of dodecanedioic acid. (+)-Decanoylcarnitine inhibits palmitate oxidation but not the oxidation of dodecanedioic acid. At low concentrations of [carboxyl-14C]dodecanedioic acid or of [1-14C]palmitate, acetylsulfanilamide is more efficiently labeled by the former. High concentrations of dodecanedioic acid inhibit palmitate oxidation and the acetylation of sulfanilamide, presumably because their CoA-esters accumulate in the cytosol. These results indicate that medium-chain dicarboxylic acids are beta-oxidized mainly in the peroxisomes.
Bergseth
S
S
Institute of Medical Biochemistry, University of Oslo, Norway.
Poisson
J P
JP
Bremer
J
J
D000107:Acetylation; D000818:Animals; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003998:Dicarboxylic Acids / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D011874:Radiometry; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity; D000077145:Sulfanilamide; D013424:Sulfanilamides / Q000378:metabolism
D003998:Dicarboxylic Acids; D013424:Sulfanilamides; D000077145:Sulfanilamide