Identification of the mutations in the prfB gene of Escherichia coli K12, which confer UGA suppressor activity.
By DNA sequencing and gene dissection, it has been revealed that Su+UGA#11, the mutant prfB of E. coli (Chang et al., 1990) has a double mutation compared with the wild-type LS653: one is a base substitution from T to C at the codon 63 and the other is from G to A at the codon 79. Both mutations cause amino acid substitution, Leu63----Phe63 (L63F) and Asp79----Gly79 (D79G), and are necessary to confer the efficient UGA suppressor activity.
Wu
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Department of Biophysics, Faculty of Science, Kyoto University, Japan.
Inokuchi
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Ozeki
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D001483:Base Sequence; D003062:Codon; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D010454:Peptide Termination Factors / Q000235:genetics*; D013489:Suppression, Genetic
D003062:Codon; D004269:DNA, Bacterial; D010454:Peptide Termination Factors; C041460:peptide chain termination release factor 2