The cloning and sequence analysis of the rat serotonin-1A receptor gene.
Using a probe derived from the human 5-HT1A receptor gene (G-21), a strongly hybridizable clone was identified from a rat genomic DNA library. This clone (G21Y2) had an overall deduced amino acid sequence that was 88% homologous with that of the human 5-HT1A receptor. There was only an 18% and 19% amino acid homology with the rat 5-HT1C and 5-HT2 receptors, respectively. The G21Y2 appears to be an excellent candidate for the rat 5-HT1A receptor gene. As this manuscript was being prepared a report by Albert et al. (13) was published in which a probe to the hamster beta 2-adrenergic receptor was used to identify a rat genomic (Clone D) that demonstrated a sequence that was similar to G21Y2.
Fujiwara
Y
Y
Department of Pharmacology, College of Medicine, University of Arizona, Tucson 85724.
Nelson
D L
DL
Kashihara
K
K
Varga
E
E
Roeske
W R
WR
Yamamura
H I
HI
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D051381:Rats; D011985:Receptors, Serotonin / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D011985:Receptors, Serotonin