title: Characterization of two new monoclonal antibodies against Haemophilus paragallinarum serovar C hemagglutinating antigen.
abstract: Two new monoclonal antibodies (MAbs), D6D8D5 and B3E6F9, both directed against Haemophilus paragallinarum serovar C hemagglutinating (HA) antigen, were produced, and characteristics of the MAbs were compared with those of the previously described MAb F2E6 in dot-blot and hemagglutination-inhibition (HI) tests using two representative H. paragallinarum strains each of serovars A, B, and C strains and 55 Japanese serovar C field isolates. MAb D6D8D5 and MAb F2E6 reacted with all serovar C strains and field isolates in the dot-blot test. However, MAb D6D8D5 showed various degrees of inhibition of the HA activity of field isolates. In the enzyme-linked immunosorbent assay-competition test, MAb D6D8D5 did not compete with MAb F2E6. MAb B3E6F9 reacted with strain S1, serovar C but not with strain Modesto, serovar C in both dot-blot and HI tests. Three out of 55 field isolates did not react with MAb B3E6F9. Neither MAb reacted with the serovar A and B strains.
authors.affiliation: Aburahi Laboratories, Shionogi & Co., Ltd., Shiga, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: S
authors.initials: S
authors.lastname: Takigami
authors.forename: Y
authors.initials: Y
authors.lastname: Iritani
authors.forename: Y
authors.initials: Y
authors.lastname: Hayashi
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D001667:Binding, Competitive; D002850:Chromatography, Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006190:Haemophilus / Q000276:immunology*; D006384:Hemagglutination; D006385:Hemagglutination Inhibition Tests; D015151:Immunoblotting
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial