title: Determination of cysteine on low-density lipoproteins using the fluorescent probe, 5-iodoacetamidofluoresceine.
abstract: Using the fluorescent sulfhydryl probe, 5-iodoacetamidofluoresceine, to label the free sulfhydryl of low-density lipoprotein, the positions of two cysteine residues in apolipoprotein B were located. The tryptic peptides containing the fluorescent probe were isolated by high-performance liquid chromatography systems and sequenced by automatic techniques. The free cysteine residues of apolipoprotein B-100 on low-density lipoprotein are located at positions 3734 and 4190, either or both of which can potentially form a disulfide linkage with apolipoprotein(a) in lipoprotein(a).
authors.affiliation: Department of Medicine, Baylor College of Medicine, Methodist Hospital, Houston, TX 77030.
authors.forename: R D
authors.initials: RD
authors.lastname: Coleman
authors.forename: T W
authors.initials: TW
authors.lastname: Kim
authors.forename: A M
authors.initials: AM
authors.lastname: Gotto
authors.forename: C Y
authors.initials: CY
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D001055:Apolipoproteins B; D003545:Cysteine; D005452:Fluoresceins; D006801:Humans; D008077:Lipoproteins, LDL; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis; D013050:Spectrometry, Fluorescence; D013439:Sulfhydryl Reagents
chemical_list: D001055:Apolipoproteins B; D005452:Fluoresceins; D008077:Lipoproteins, LDL; D010446:Peptide Fragments; D013439:Sulfhydryl Reagents; C015296:5-iodoacetamidofluorescein; D003545:Cysteine