title: [Directed reconstruction of the influenza virus hemagglutinin gene].
abstract: A new approach to create chimeric genes by directed exchange of oligonucleotide fragments was developed. By oligonucleotide-directed mutagenesis a few deletion mutants of the influenza virus hemagglutinin (HA) gene were obtained. These variants of HA gene contain unique restriction sites in DNA regions coding for the A and B epitopes of the HA molecule. The obtained special vectors may be used for cloning DNA fragments coding for new amino acid sequences in internal sites of the HA gene.
authors.forename: V A
authors.initials: VA
authors.lastname: Petrenko
authors.forename: S M
authors.initials: SM
authors.lastname: Kipriianov
authors.forename: L N
authors.initials: LN
authors.lastname: Semenova
authors.forename: A N
authors.initials: AN
authors.lastname: Boldyrev
authors.forename: P I
authors.initials: PI
authors.lastname: Pozdniakov
authors.forename: A M
authors.initials: AM
authors.lastname: Eroshkin
authors.forename: G F
authors.initials: GF
authors.lastname: Sivolobova
mesh_terms: D001483:Base Sequence; D002678:Chimera; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006389:Hemagglutinins, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009975:Orthomyxoviridae / Q000378:metabolism*; D015183:Restriction Mapping
chemical_list: D004274:DNA, Recombinant; D006389:Hemagglutinins, Viral
vernacular_title: Napravlennaia rekonstruktsiia gena gemaggliutinina virusa grippa.