Amplification of a long sequence that includes a processed pseudogene for elongation factor 2 in the mouse.
Quantitative Southern blotting analysis has demonstrated that mouse cells contain about 70 copies per haploid genome of a DNA sequence related to the gene for elongation factor 2. The restriction maps of seven cosmids that each carry one copy of the EF2-related sequence (MER) and nucleotide sequences of MERs were highly conserved among the cosmids. Data obtained by such analyses suggest that MERs were produced by the integration of one copy of MER derived from poly(A)+ mRNA for EF2 into a specific site in the mouse genome, with subsequent amplification of MER together with its large flanking sequences during the evolution of the mouse. Furthermore, it appears that the size of each repeating unit is more than 60 kb. Analysis by pulse-field gel electrophoresis suggested that multiple copies of a repeating unit of more than 400 kb (or two units) are clustered at a specific site (or each specific site) in the mouse genome.
Koide
T
T
Department of Cell Biology, National Institute for Basic Biology, Aichi, Japan.
Ishiura
M
M
Hazumi
N
N
Shiroishi
T
T
Okada
Y
Y
Uchida
T
T
D000818:Animals; D001483:Base Sequence; D003360:Cosmids / Q000235:genetics; D004586:Electrophoresis; D005784:Gene Amplification; D051379:Mice; D008969:Molecular Sequence Data; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors / Q000235:genetics*; D011544:Pseudogenes; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors