Identification of two nerve growth factor-induced polypeptides in PC12 cells.
We have identified two nerve growth factor (NGF)-induced polypeptides (Mr 80,000 and 90,000) in PC12 cells that are heat stable and not sulfated. Indirect immunofluorescence localization of these polypeptides in NGF-treated PC12 cells reveals a punctate pattern concentrated in neurites. Immunoperoxidase staining of rat tissue shows that these polypeptides are found throughout the brain in selected subsets of neurons and are absent in the pituitary or adrenal medulla. Several of these characteristics are similar to a recently described NGF-induced secretory protein (VGF8a), which has sequence similarities to secretogranins. The property of NGF inducibility and the distribution of these polypeptides within rat tissues are both novel features for secretogranin proteins.
Sussman
M A
MA
Department of Molecular Biology, Scripps Clinic and Research Foundation, La Jolla, CA 92037.
Battenberg
E
E
Bloom
F E
FE
Fowler
V M
VM
D000310:Adrenal Gland Neoplasms; D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002460:Cell Line; D002864:Chromogranins; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D010452:Peptide Biosynthesis; D010455:Peptides / Q000302:isolation & purification; D010673:Pheochromocytoma; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification; D051381:Rats
D002864:Chromogranins; D007106:Immune Sera; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D010455:Peptides; D011506:Proteins