A cluster of hematopoietic serine protease genes is found on the same chromosomal band as the human alpha/delta T-cell receptor locus.
The chymotrypsin-like family of serine protease genes includes several members that are expressed exclusively in subsets of hematopoietic cells. For example, human neutrophil elastase and cathepsin G are expressed only in myelomonocytic precursors, and cytotoxic-T-cell serine proteases are found only in cytotoxic lymphocytes. We have used a cathepsin G cDNA probe to clone two cathepsin G-like genes (designated CGL-1 and CGL-2) from a human genomic library. We have determined that CGL-1 is identical to a previously identified gene (known as CCPI, CTLA I, or cytotoxic serine protease B) that is expressed only in activated cytotoxic T lymphocytes. We show here that cathepsin G, CGL-1, and CGL-2 are linked on an approximately 50-kilobase locus found on human chromosome 14 at band q11.2. This gene cluster maps to the same chromosomal band as the alpha and delta T-cell receptor genes; this region is involved in most chromosomal translocations and inversions that are specifically associated with T-cell malignancies.
Hanson
R D
RD
Department of Medicine, Jewish Hospital at Washington University Medical Center, St. Louis, MO 63110.
Hohn
P A
PA
Popescu
N C
NC
Ley
T J
TJ
D015139:Blotting, Southern; D056649:Cathepsin G; D002403:Cathepsins / Q000235:genetics*; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002883:Chromosomes, Human, Pair 14; D003360:Cosmids; D008040:Genetic Linkage; D015894:Genome, Human; D006412:Hematopoietic Stem Cells / Q000201:enzymology*; D006801:Humans; D046911:Macromolecular Substances; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D015183:Restriction Mapping; D012697:Serine Endopeptidases / Q000235:genetics*
D046911:Macromolecular Substances; D011948:Receptors, Antigen, T-Cell; D002403:Cathepsins; D012697:Serine Endopeptidases; C534094:CTSG protein, human; D056649:Cathepsin G