Human genetically polymorphic deoxyribonuclease: purification, characterization, and multiplicity of urine deoxyribonuclease I.
A deoxyribonuclease I was purified from the urine of a 46-year-old male (a single individual) by using a series of column chromatographies to a homogeneous state as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The enzyme was found to be a glycoprotein, containing 1 fucose, 7 galactose, 10 mannose, 6 glucosamine, and 2 sialic acid residues per molecule. The N-terminal amino acid sequence up to the 27th residue of the enzyme was similar to that of pancreatic deoxyribonuclease I from bovine and other species. The catalytic properties of the enzyme derived from a single individual closely resembled those of deoxyribonuclease I purified from human urine collected from several volunteers [Ito, K. et al. (1984) J. Biochem. 95, 1399-1406]. The purified enzyme was found to consist of multiple forms with different pI values. These findings are compatible with the existence of genetic polymorphism of deoxyribonuclease I in human urine previously reported [Kishi, K. et al. (1989) Hum. Genet. 81, 295-297]. This multiplicity of the urine enzyme might be due to variations in the primary structure and/or differences in the content of sialic acid.
Yasuda
T
T
Department of Legal Medicine, Fukui Medical School.
Awazu
S
S
Sato
W
W
Iida
R
R
Tanaka
Y
Y
Kishi
K
K
D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology; D002241:Carbohydrates / Q000737:chemistry; D003850:Deoxyribonuclease I / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000652:urine*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000652:urine*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D013379:Substrate Specificity
D000906:Antibodies; D002241:Carbohydrates; D007527:Isoenzymes; D003850:Deoxyribonuclease I