Interleukin production by human colostral cells after in vitro mitogen stimulation.
Human colostral cells were pulsed with PHA, Con A, or LPS and cultivated in serum-free medium. The culture supernatants were tested for IL-1 activity in C3H/HeJ thymocyte assay and for IL-2 activity on human lymphoblasts. The IL-1 activity was the highest at the 24th h of cultivation and IL-2 activity at the 48th h of cultivation.
Nikolova
E
E
Institute of Cell Biology and Morphology, Bulgarian Academy of Sciences, Sofia.
Staykova
M
M
Raicheva
D
D
Karadjova
M
M
Neronov
A
A
Ivanov
I
I
Goranov
I
I
D001681:Biological Assay; D003126:Colostrum / Q000166:cytology / Q000276:immunology*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000032:analysis / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000032:analysis / Q000096:biosynthesis*; D008264:Macrophages / Q000276:immunology; D008934:Mitogens / Q000494:pharmacology; D011247:Pregnancy; D013601:T-Lymphocytes / Q000276:immunology
D007375:Interleukin-1; D007376:Interleukin-2; D008934:Mitogens