Production of interleukin-1 by draining lymph node cells during the induction phase of contact sensitization in mice.
Biologically active interleukin-1 (IL-1) has been detected in supernatants of draining lymph node cells isolated from contact-sensitized mice. Induction of IL-1 was dependent upon the concentration of sensitizer applied and occurred within 2 hr of exposure. The IL-1 activity could not be attributed to other interleukins and was neutralized by a specific antiserum. Reduced concentrations of IL-1 were produced by cells isolated from the draining nodes of mice that had been pre-exposed to the sensitizer. Since IL-1 has the potential to influence several aspects of lymphocyte activation, the production of significant concentrations of biologically active IL-1 by draining lymph node cells indicates that it is likely to play an important role in the afferent phase of contact sensitization.
Hopkins
S J
SJ
University of Manchester Rheumatic Diseases Centre, Hope Hospital, Salford, U.K.
Humphreys
M
M
Kinnaird
A
A
Jones
D A
DA
Kimber
I
I
D000818:Animals; D002455:Cell Division / Q000276:immunology; D002478:Cells, Cultured; D003877:Dermatitis, Contact / Q000276:immunology*; D004431:Ear, External; D007375:Interleukin-1 / Q000096:biosynthesis*; D007700:Kinetics; D008198:Lymph Nodes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010081:Oxazolone / Q000276:immunology
D007375:Interleukin-1; D010081:Oxazolone