Bronze baby syndrome: an animal model.
We evaluated the appropriateness of an animal model for the bronze baby syndrome. Ligation of the common bile duct in adult Wistar rats induces an accumulation of porphyrins and copper in the liver and a 20% conversion of protoporphyrin IX into (Cu(II)-protoporphyrin IX. Upon irradiation of these animals with super-blue lamps, the plasma content of Cu(II)-protoporphyrin increases by about 30%. Cholestasis also increases the recovery of porphyrins in the urine, although light treatment of ligated rats further increases urinary porphyrin excretion. The spectroscopic changes induced by irradiation of sera of ligated rats are consistent with the formation of products that have the typical spectrum found in bronze baby syndrome patients, i.e. a reduced absorbance in the visible region and an increased absorption in near-UV and red spectral regions. The products responsible for the brown discoloration found in bronze baby syndrome seem to result from phototransformation of copper-porphyrins subsequent to an electron transfer between photoexcited bilirubin and the copper ion.
Jori
G
G
Department of Pediatrics, University of Padova, Italy.
Reddi
E
E
Rubaltelli
F F
FF
D000818:Animals; D001663:Bilirubin / Q000378:metabolism*; D001651:Cholestasis, Extrahepatic / Q000378:metabolism*; D003137:Common Bile Duct Diseases / Q000378:metabolism*; D003300:Copper / Q000378:metabolism*; D004195:Disease Models, Animal; D008297:Male; D011166:Porphyrins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D011166:Porphyrins; D003300:Copper; D001663:Bilirubin