The role of the membrane skeleton in concanavalin A-mediated agglutination of human erythrocytes.
In the erythrocyte membrane, the mobility of band 3 protein, the receptor for concanavalin A (Con A), is drastically reduced by the membrane skeleton. Yet, the vesicles free of membrane skeletal proteins, isolated from the highly agglutinable proteinase-treated cells, are found to be devoid of Con A agglutinability. The vesicles bind Con A in normal amounts, and remain agglutinable with the wheat germ and Ricinus agglutinins. Intracellular entrapment of monospecific antibodies to spectrin and 4.1 protein (two of the major skeletal components of the membrane) is also found to inhibit agglutination by 30-50%. Thus the membrane skeleton appears to play a positive role in the agglutination of the cells with Con A. The anti-ankyrin antibodies are found to be without any effect. The anti-band 3 (cytoplasmic domain) antibodies are also inhibitory to agglutination. Since Con A binding to cells alters the shape responses and deformability of the cells, and the cells resist fragmentation at 49 degrees C, the properties of the whole skeleton, especially spectrin, appear to be changed. The Con A-bound membranes also do not release the complex of spectrin-band 4.1-actin when extracted with a hypotonic medium. It appears that Con A binding leads to interaction of the cytoplasmic domain of the receptor with a skeletal component, possibly spectrin. Subsequent to this, the receptor molecules and the skeletal proteins undergo aggregation in the membrane, which is detected by their crosslinking by an 8.6-A span bifunctional reagent. The contractility believed to be associated with the membrane skeleton may be responsible for the aggregation.
Pestonjamasp
K N
KN
Biological Chemistry Division, Tata Memorial Centre, Parel, Bombay, India.
Gokhale
S M
SM
Mehta
N G
NG
D001457:Anion Exchange Protein 1, Erythrocyte / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology*; D006384:Hemagglutination / Q000187:drug effects*; D006801:Humans; D008565:Membrane Proteins / Q000097:blood / Q000502:physiology*
D001457:Anion Exchange Protein 1, Erythrocyte; D008565:Membrane Proteins; D003208:Concanavalin A