title: DNA photorepair: chromophore composition and function in two classes of DNA photolyases.
abstract: DNA photolyase catalyzes the repair of pyrimidine dimers in UV-damaged DNA in a reaction which requires visible light. Class I photolyases (Escherichia coli, yeast) contain 1,5-dihydroFAD (FADH2) plus a pterin derivative (5,10-methenyltetrahydropteroylpolyglutamate). In class II photolyases (Streptomyces griseus, Scenedesmus acutus, Anacystis nidulans, Methanobacterium thermoautotrophicum) the pterin chromophore is replaced by an 8-hydroxy-5-deazaflavin derivative. The two classes of enzymes exhibit a high degree of amino acid sequence homology, suggesting similarities in protein structure. Action spectra studies show that both chromophores in each enzyme tested act as sensitizers in catalysis. Studies with E. coli photolyase show that the pterin chromophore is not required when FADH2 acts as the sensitizer but that FADH2 is required when the pterin chromophore acts as sensitizer. FADH2 is probably the chromophore that directly interacts with substrate in a reaction which may be initiated by electron transfer from the excited singlet state (1FADH2*) to form a flavin radical plus an unstable pyrimidine dimer radical. Pterin, the major chromophore in E. coli photolyase, may act as an antenna to harvest light energy which is then transferred to FADH2.
authors.affiliation: Department of Biological Chemistry, Hahnemann University School of Medicine, Philadelphia, PA 19102.
authors.forename: M S
authors.initials: MS
authors.lastname: Jorns
mesh_terms: D001704:Biopolymers; D002384:Catalysis; D004247:DNA / Q000528:radiation effects*; D004260:DNA Repair; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000737:chemistry* / Q000145:classification; D015394:Molecular Structure; D013053:Spectrophotometry
chemical_list: D001704:Biopolymers; D004247:DNA; D004255:Deoxyribodipyrimidine Photo-Lyase