Thrombopoietic activity of human interleukin-6.
Thrombopoietin (TPO), a regulatory factor in platelet production, was purified from the conditioned medium of TNK-01 cells cultured in the presence of human interleukin-1. The N-terminal sequence of purified TPO was determined to be VPPGEDSKDVAAPHRQPLT, identical to that of the N-terminal region of human interleukin-6 (IL-6). Two forms of TPO with molecular masses of 24 and 27 kDa were identified as IL-6 by Western analysis using an anti-IL-6 antibody. Commercial recombinant human IL-6 produced in Escherichia coli, stimulated megakaryocyte colony formation in the presence of mouse interleukin-3 and increased the number of peripheral platelets in mice in a dose-dependent manner. From these results, it is concluded that human IL-6 has thrombopoietic activity.
Nagasawa
T
T
Institute of Clinical Medicine, University of Tsukuba, Ibaraki, Japan.
Orita
T
T
Matsushita
J
J
Tsuchiya
M
M
Neichi
T
T
Imazeki
I
I
Imai
N
N
Ochi
N
N
Kanma
H
H
Abe
T
T
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D003114:Colony-Forming Units Assay; D004586:Electrophoresis / Q000379:methods; D006023:Glycoproteins / Q000302:isolation & purification*; D006801:Humans; D015850:Interleukin-6 / Q000032:analysis* / Q000494:pharmacology; D008080:Liposarcoma / Q000378:metabolism; D051379:Mice; D010976:Platelet Count / Q000187:drug effects*; D011994:Recombinant Proteins / Q000032:analysis; D013926:Thrombopoietin / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000596:Amino Acids; D006023:Glycoproteins; D015850:Interleukin-6; D011994:Recombinant Proteins; D013926:Thrombopoietin