Histochemical detection of sugar residues in the chick embryo mesonephros with lectin-horseradish peroxidase conjugates.
Fragments of mesonephros were taken from chick embryos and studied from the 4th to the 21st day of incubation. A battery of seven different horseradish peroxidase-labelled lectins was used to study the distribution of carbohydrate residues in glycoconjugates along the mesonephric nephron during the period of excretory activity and the period of involution. ConA and WGA reacted at every site of the nephron thus showing the ubiquitous presence of alpha-D-mannose and N-acetyl-D-glucosamine. SBA was a good marker of the proximal tubule. Other lectins, such as PNA and LTA, reacted only for a short time at some sites during the considered period of incubation. The presence of sialic acid was detected in the podocytes, capillary wall and mesangial cells. From the 10th-11th day of incubation changes were noted in the proximal tubule as shown by PNA reactivity. This may be significant as regards the exact stage of incubation during which the involution of mesonephros begins.
Gheri
G
G
Department of Human Anatomy and Histology, University of Florence, Italy.
Bryk
S G
SG
Petrelli
V
V
D000117:Acetylglucosamine / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis*; D002642:Chick Embryo; D006001:Glycoconjugates / Q000032:analysis*; D006651:Histocytochemistry; D006735:Horseradish Peroxidase; D037102:Lectins / Q000378:metabolism*; D008358:Mannose / Q000032:analysis; D008650:Mesonephros / Q000737:chemistry* / Q000378:metabolism; D012794:Sialic Acids / Q000032:analysis
D002241:Carbohydrates; D006001:Glycoconjugates; D037102:Lectins; D012794:Sialic Acids; D006735:Horseradish Peroxidase; D008358:Mannose; D000117:Acetylglucosamine