title: Steroid receptors in the developing and the adult rabbit endocervix and in endocervical epithelial cells isolated by flow cytometry.
abstract: Immunocytochemistry with monoclonal antibodies H222 and JZB39 was used to study nuclear estrogen (ER) and progesterone (PgR) receptors, respectively, in the cervix during differentiation and in the adult rabbit. The undifferentiated state of the cervix of 2-week-old rabbits correlates with a paucity of immunoreactive nuclear ER, while the epithelium of most of these animals showed moderate immunostaining for the nuclear PgR. The cervical epithelium, stroma and muscle cells of 1-month-old rabbits, showed weak immunostaining for the ER, while staining for PgR remained comparable to that of 2-week-old rabbits. For 2-4-month old rabbits the epithelium was characterized by moderate immunostaining for the nuclear ER and strong immunostaining for the PgR. Strong, heterogeneous immunostaining for nuclear ER and PgR receptors in endocervical epithelial cells from 6-month-old (adult), estrous rabbits suggested there are subpopulations of cells that express differential sensitivity to steroid hormones. In order to characterize such subpopulations, live endocervical epithelial cells were sorted with a flow cytometer on the basis of forward angle light scatter (FSC) and side scatter (SSC) signals which correlated with cell size and secretory granule content, respectively. Secretory cells, as verified by ultrastructural analysis and histochemical staining, expressed the highest FSC and SSC signals and were designated fraction "a". Changes in the hormonal status of the animals altered the intrinsic light scatter properties of fraction "a" cells as follows: maximum FSC and SSC signals were reported for cells from estrous animals; ovariectomy or progesterone-dominance decreased cell size (FCS) and secretory granule content (SSC), while treatment of ovariectomized rabbits with estradiol increased both parameters. When fraction "a" cells from estrous rabbits were incubated with the monoclonal antibodies, two distinct subpopulations of secretory cells were identified by intensity and pattern of nuclear staining for the ER and PgR. Changes in the hormonal status of the animals produced changes in the intensity of nuclear immunostaining, however both cell types remained distinguishable on the basis of immunostain pattern reflecting either permanent or transitory differences in them, and differential hormone sensitivity. The presence of nuclear ER and PgR proteins in these cells confirms their function is bireceptor-mediated.
authors.affiliation: Department of Cell Biology-Anatomy, Texas Tech University, Health Sciences Center, Lubbock 79430.
authors.forename: B S
authors.initials: BS
authors.lastname: Chilton
authors.forename: L S
authors.initials: LS
authors.lastname: Geis
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D002584:Cervix Uteri / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D004848:Epithelium / Q000648:ultrastructure; D004958:Estradiol / Q000494:pharmacology; D004971:Estrus; D005260:Female; D005434:Flow Cytometry; D011374:Progesterone / Q000494:pharmacology; D011817:Rabbits; D011960:Receptors, Estrogen / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000276:immunology / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D011374:Progesterone; D004958:Estradiol