title: Serologic evidence of human immunodeficiency virus infection of the central nervous system in African patients with acquired immunodeficiency syndrome.
abstract: The intrathecal synthesis of antibodies to human immunodeficiency virus (HIV) was determined by 3 different immunoassays in 15 African patients with pre-acquired immunodeficiency syndrome (AIDS) and AIDS. Isoelectric focusing together with affinity-mediated immunoblot were found to be superior to enzyme-linked immunosorbent assay and Western blot in providing information not only about the antigen specificity of locally produced antibodies but also about their clonal distribution. In 9 of the subjects, HIV-specific oligoclonal bands were demonstrable with higher frequency or intensity in the cerebrospinal fluid than in the autologous serum, indicating autochthonous synthesis of HIV-related antibodies.
authors.affiliation: Institut für Virologie und Immunbiologie der Universität Würzburg, BRD.
authors.forename: R
authors.initials: R
authors.lastname: Kaiser
authors.forename: R
authors.initials: R
authors.lastname: Dörries
authors.forename: J
authors.initials: J
authors.lastname: ter Meulen
authors.forename: J J
authors.initials: JJ
authors.lastname: Kidenya
authors.forename: M
authors.initials: M
authors.lastname: Pöllath
authors.forename: K
authors.initials: K
authors.lastname: Fleischer
authors.forename: V
authors.initials: V
authors.lastname: ter Meulen
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000150:complications* / Q000276:immunology; D000328:Adult; D015153:Blotting, Western; D002493:Central Nervous System Diseases / Q000276:immunology / Q000382:microbiology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015483:HIV Antibodies / Q000032:analysis* / Q000096:biosynthesis; D006801:Humans; D015151:Immunoblotting; D007525:Isoelectric Focusing; D008297:Male; D008875:Middle Aged; D013636:Tanzania
chemical_list: D015483:HIV Antibodies