Characterization of two plasmids arising spontaneously in phosphate-limited continuous cultures of Escherichia coli HB101[pAT153].
Two derivatives of plasmid pAT153 which arose spontaneously in phosphate-limited continuous cultures of Escherichia coli HB101[pAT153] have been characterised. The smaller plasmid, pLAB-446, resulted from a deletion of 446 base pairs of DNA covering the translation start and codon sequence of the tetracycline resistance gene. The other plasmid, pLAB135, differed from pAT153 only by an A:T to G:C transition in the transcribed but untranslated leader region of the tet gene which decreased tet transcription by 84%, possibly due to the formation of a more stable stem-loop structure in the mRNA.
Brownlie
L
L
School of Biochemistry, University of Birmingham, U.K.
Stephenson
J R
JR
Cole
J A
JA
D001483:Base Sequence; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D010710:Phosphates / Q000378:metabolism; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D013753:Tetracycline Resistance; D014158:Transcription, Genetic
D003470:Culture Media; D004269:DNA, Bacterial; D010710:Phosphates