Characterization of the subunit particles of feline calicivirus.
In the culture fluid from cells infected with feline calicivirus (FCV) F4 strain, the infectious and smaller non-infectious subunit particles were detected by complement fixation (CF) test after sucrose gradient centrifugation. The results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblot analyses confirmed the existence of the subunit particles of FCV, and showed that the infectious and subunit particles were mainly composed of 65K capsid protein. The subunit particles were further purified by ion-exchange chromatography and sucrose gradient centrifugation. The purified subunit and infectious particles had the same neutralizing epitope on 65K protein detected by immunoblot analysis with a neutralizing monoclonal antibody. Antigenic comparison between the infectious and subunit particles by the CF tests using an antiserum against heterologous strain of FCV F14 indicated that the subunit particles might have more highly conserved antigens of FCV than the infectious particles.
Tohya
Y
Y
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Tsubakimoto
M
M
Horimoto
T
T
Takahashi
E
E
Mikami
T
T
D000818:Animals; D002139:Caliciviridae / Q000276:immunology; D002213:Capsid / Q000032:analysis; D002415:Cats; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D002852:Chromatography, Ion Exchange; D003168:Complement Fixation Tests; D014771:Virion / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*