Antibodies raised against different oligopeptide segments of human dopamine-beta-hydroxylase.
We raised antibodies against 3 oligopeptide segments of human dopamine-beta-hydroxylase (hDBH) corresponding to the N-terminal (hDBH-N), the intermediate (hDBH-I), and the C-terminal (hDBH-C) amino acid sequences (residues 26-43, 452-468, and 582-598), respectively. We characterized the antibodies in terms of specificity by means of Western blotting and immunohistochemistry. Anti-hDBH-N antiserum recognized DBH in the brain (noradrenergic neurons in the pons and medulla oblongata) and adrenal medulla, not only of human but also of mouse, rat and house shrew. In contrast, anti-hDBH-C antiserum recognized only human DBH. These observations suggest that the antibody raised against the hDBH-C terminal peptide may specifically recognize only human DBH.
Nagatsu
I
I
Department of Anatomy, Fujita Health University School of Medicine, Toyoake, Japan.
Kobayashi
K
K
Fujii
T
T
Komori
K
K
Sekiguchi
K
K
Titani
K
K
Fujita
K
K
Nagatsu
T
T
D000311:Adrenal Glands / Q000201:enzymology*; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D001345:Autoradiography; D015153:Blotting, Western; D002417:Cattle; D004299:Dopamine beta-Hydroxylase / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007457:Iodine Radioisotopes; D008125:Locus Coeruleus / Q000201:enzymology*; D008297:Male; D051379:Mice; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis*; D012689:Sequence Homology, Nucleic Acid
D000906:Antibodies; D007457:Iodine Radioisotopes; D010455:Peptides; D004299:Dopamine beta-Hydroxylase