title: Evaluation of the Ames Seralyzer for the determination of carbamazepine, phenobarbital, and phenytoin concentrations in saliva.
abstract: The performance of the dry-phase apoenzyme reactivation immunoassay system (ARIS) for the measurement of carbamazepine (CBZ), phenobarbital (PB), and phenytoin (PHT) concentrations in saliva was compared with fluorescence polarization immunoassay (FPIA). Blood and saliva samples were collected from 163 adult and pediatric epilepsy patients, then analyzed using both methods. Regressions between ARIS saliva CBZ, PB, and PHT concentrations, and FPIA unbound and total serum concentrations were highly correlated, but the ARIS technique was somewhat less precise than the FPIA. Valproic acid co-medication did not affect the relationships between ARIS and FPIA saliva concentrations and unbound serum concentrations of PHT, but did disrupt the relationship between ARIS and FPIA saliva PHT and total serum PHT. The sensitivity, specificity, predicted value positive (PV+) of a therapeutic concentration, and predicted value negative (PV-) of a concentration outside the therapeutic range for the ARIS saliva technique compared very well with FPIA for CBZ, PB, and PHT. The ARIS technique for CBZ, PB, and PHT saliva determination provides acceptable accuracy, precision, and sensitivity for therapeutic monitoring. In practice, the benefits of the ARIS saliva technique, including ease of collection, safety, patient/parent acceptance, and short analysis time, are striking.
authors.affiliation: Division of Pharmacy Practice, School of Pharmacy, University of North Carolina, Chapel Hill 27599.
authors.forename: M V
authors.initials: MV
authors.lastname: Miles
authors.forename: M B
authors.initials: MB
authors.lastname: Tennison
authors.forename: R S
authors.initials: RS
authors.lastname: Greenwood
authors.forename: S E
authors.initials: SE
authors.lastname: Benoit
authors.forename: M D
authors.initials: MD
authors.lastname: Thorn
authors.forename: J A
authors.initials: JA
authors.lastname: Messenheimer
authors.forename: A L
authors.initials: AL
authors.lastname: Ehle
mesh_terms: D002220:Carbamazepine / Q000032:analysis* / Q000097:blood; D005069:Evaluation Studies as Topic; D016231:Fluorescence Polarization Immunoassay / Q000295:instrumentation*; D006801:Humans; D010634:Phenobarbital / Q000032:analysis* / Q000097:blood; D010672:Phenytoin / Q000032:analysis* / Q000097:blood; D012463:Saliva / Q000378:metabolism*
chemical_list: D002220:Carbamazepine; D010672:Phenytoin; D010634:Phenobarbital