Determination of cysteine on low-density lipoproteins using the fluorescent probe, 5-iodoacetamidofluoresceine.
Using the fluorescent sulfhydryl probe, 5-iodoacetamidofluoresceine, to label the free sulfhydryl of low-density lipoprotein, the positions of two cysteine residues in apolipoprotein B were located. The tryptic peptides containing the fluorescent probe were isolated by high-performance liquid chromatography systems and sequenced by automatic techniques. The free cysteine residues of apolipoprotein B-100 on low-density lipoprotein are located at positions 3734 and 4190, either or both of which can potentially form a disulfide linkage with apolipoprotein(a) in lipoprotein(a).
Coleman
R D
RD
Department of Medicine, Baylor College of Medicine, Methodist Hospital, Houston, TX 77030.
Kim
T W
TW
Gotto
A M
AM
Yang
C Y
CY
D000595:Amino Acid Sequence; D001055:Apolipoproteins B; D003545:Cysteine; D005452:Fluoresceins; D006801:Humans; D008077:Lipoproteins, LDL; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis; D013050:Spectrometry, Fluorescence; D013439:Sulfhydryl Reagents
D001055:Apolipoproteins B; D005452:Fluoresceins; D008077:Lipoproteins, LDL; D010446:Peptide Fragments; D013439:Sulfhydryl Reagents; C015296:5-iodoacetamidofluorescein; D003545:Cysteine