An immunoassay for the determination of total IgA subclass antibodies in human serum.
An enzyme immunoassay capable of determining total IgA1 and IgA2 concentrations in human serum has been developed. Subclass-specific monoclonal antibodies are bound to polyacrylamide bead-conjugated anti-mouse immunoglobulin antibodies. Bound IgA is detected with an anti-IgA peroxidase conjugate and the standard curve is linear in the region 0.25 - 2.0 micrograms/ml. Coefficient of variation values range from 0.24 - 5.77% for the IgA1 standard curve and from 0.86 - 5.92% for the IgA2 standard curve. Inter-assay variation for the IgA1 and IgA2 control sample values were 8.2% and 13.4%, respectively.
Haun
M
M
Canadian Red Cross Society, National Reference Laboratory, Ottawa, Ontario.
Wasi
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000032:analysis* / Q000145:classification; D051379:Mice; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D007070:Immunoglobulin A