Trophic effect of granulocyte-macrophage colony-stimulating factor on central cholinergic neurons in vitro.
Granulocyte-macrophage colony-stimulating factor (GM-CSF) elevated choline acetyltransferase (ChAT) activities of mouse septal neurons as well as of cholinergic hybridoma line cells SN6.10.2.2 in vitro. It augmented ChAT activities and neurite extension of interleukin 3-activated cholinergic neurons. Thus, GM-CSF should be added as a trophic factor for central cholinergic neurons.
Kamegai
M
M
Division of Demyelinating Disease and Aging, National Institute of Neuroscience, NCNP, Tokyo, Japan.
Konishi
Y
Y
Tabira
T
T
D000818:Animals; D002478:Cells, Cultured; D002795:Choline O-Acetyltransferase / Q000032:analysis* / Q000187:drug effects; D004347:Drug Interactions; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006825:Hybridomas; D007377:Interleukin-3 / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009474:Neurons / Q000187:drug effects* / Q000201:enzymology; D011994:Recombinant Proteins / Q000494:pharmacology
D007377:Interleukin-3; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D002795:Choline O-Acetyltransferase