Direct identification and characterization of llama (Lama glama L.) whey proteins by microsequencing after western blotting.
Amino acid sequence determination is the most reliable and powerful tool to identify a protein or to classify a new one by comparison of its primary structure with already known sequences. A rapid and simple purification procedure is an essential pre-requisite for routine sequence determination. Structural characterization of llama whey proteins was undertaken for evolutionary as well as economic purposes. N-terminal sequence analyses directly on an immobilon polyvinylidene difluoride (PVDF) membrane, following Western blotting of both native and SDS-denatured llama whey proteins after polyacrylamide gel electrophoresis, revealed three different forms of glycosylated alpha-lactalbumin, and a protein with a high degree of homology with a camel whey protein of unknown function. Furthermore, by immunoblotting techniques, the electrophoretic band corresponding to serum albumin was identified.
Cantisani
A
A
Centro Studi Alimentazione Animali, CNR, Torino, Italy.
Napolitano
L
L
Giuffrida
M G
MG
Conti
A
A
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002161:Camelids, New World / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007768:Lactalbumin / Q000737:chemistry / Q000302:isolation & purification; D008894:Milk Proteins / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D012709:Serum Albumin / Q000737:chemistry / Q000302:isolation & purification; D013045:Species Specificity; D000067816:Whey Proteins
D008894:Milk Proteins; D012709:Serum Albumin; D000067816:Whey Proteins; D007768:Lactalbumin