[Cloning genes for biosynthesis of Pseudomonas putida tryptophan in Escherichia coli cells].
The trpE, trpC and trpIBA genes of Pseudomonas putida were cloned by complementation of the corresponding auxotrophic mutations of Escherichia coli using pBR322 as a vector. With the exception of trpE, transcription of all genes in new host takes place under control of their own promoters. Expression of the trpD gene linked to trpC was not registered in E. coli. Repressible trpC enzyme was synthesized constitutively in E. coli. Characteristic regulation of P. putida trpBA genes via induction by indolglycerol phosphate is retained in E. coli. The activator gene trpI and tryptophan synthase genes were closely linked in the trpIBA sequence.
Olekhnovich
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Fomichev
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D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics / Q000378:metabolism*; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014364:Tryptophan / Q000096:biosynthesis*; D014367:Tryptophan Synthase / Q000378:metabolism
D004269:DNA, Bacterial; D014364:Tryptophan; D014367:Tryptophan Synthase
Klonirovanie genov biosinteza triptofana Pseudomonas putida v kletkakh Escherichia coli.