The teratogenic effects of E-64 on rat embryogenesis.
To examine the effects of E-64 (a thiol protease inhibitor) on the development of rat embryos, pregnant Wistar rats were injected with 10-30 mg/kg of E-64 intra-peritoneally on days 9 and 10 of gestation. On day 21, the rats were killed and the fetuses were examined for malformations. When 30 mg/kg of E-64 was administered, the incidence of resorptions and malformations was 25.7% and 60.4%, respectively. The most common malformations were hydrocephaly, exencephaly, anophthalmia, microphthalmia, hydronephrosis and renal hypoplasia. These results suggest that thiol protease inhibitor is teratogenic during early rat embryogenesis.
Tachikura
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Department of Pediatrics, Faculty of Medicine, Kagoshima University, Japan.
D000014:Abnormalities, Drug-Induced; D000818:Animals; D015853:Cysteine Proteinase Inhibitors / Q000633:toxicity*; D005260:Female; D005333:Fetus / Q000002:abnormalities* / Q000187:drug effects; D007930:Leucine / Q000031:analogs & derivatives* / Q000633:toxicity; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains
D015853:Cysteine Proteinase Inhibitors; D007930:Leucine; C024974:E 64