Effect of anticancer drugs on the release of tumour necrosis factor in vitro.
The anticancer drugs Adriamycin, bleomycin, vincristine, 4-hydroperoxycyclophosphamide, FK156 and FK565 and the immunosuppressive drug cyclosporin-A were tested for their effect on lipopolysaccharide-induced tumour necrosis factor-alpha (TNF) release from rat peritoneal exudate cells in vitro. FK565 resulted in a marked enhancement of TNF release when added to cultures at doses of 1 microgram/ml and 10 micrograms/ml. However, FK156, Adriamycin, bleomycin, 4-hydroperoxycyclophosphamide, vincristine and cyclosporin A did not affect the release of TNF under the same conditions. Puromycin inhibited the release of TNF in the same system at non-cytotoxic doses of 1 microgram/ml and 10 micrograms/ml.
Hasan
S I
SI
Royal College of Surgeons of England, London, UK.
Ahmed
K
K
Turk
J L
JL
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D003524:Cyclosporins / Q000494:pharmacology; D008297:Male; D009842:Oligopeptides / Q000494:pharmacology; D011691:Puromycin / Q000494:pharmacology; D051381:Rats; D011917:Rats, Inbred Lew; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D000970:Antineoplastic Agents; D003524:Cyclosporins; D009842:Oligopeptides; D014409:Tumor Necrosis Factor-alpha; D011691:Puromycin; C039537:heptanoyl-gamma-D-glutamyl-L-meso-diaminopimelyl-D-alanine