title: Electrophysiology and lucifer yellow injection of nucleus gigantocellularis neurones in an isolated and perfused guinea pig brain in vitro.
abstract: Intracellular recordings from nucleus gigantocellularis (NGC) neurones were obtained in isolated and perfused whole brains of guinea pigs in vitro. A majority of cells (90%) were characterized by an action potential of short duration (0.3 ms) followed first by a fast and then by a slower afterhyperpolarization (AHP). Their firing pattern was mostly irregular. These cells were shown to have high threshold calcium spikes and plateau potentials. The other cell type represented only 10% of the recorded cells in the NGC. It was characterized by a wider (0.6 ms) action potential, a large single AHP, the presence of a transient rectification presumably due to an A-current and a rather regular resting discharge. Using Lucifer yellow injections in brainstem slices, both cell types were shown to correspond to gigantocellular neurones.
authors.affiliation: Département de Physiologie, CMU, Geneva, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Serafin
authors.forename: A
authors.initials: A
authors.lastname: Khateb
authors.forename: M
authors.initials: M
authors.lastname: Mühlethaler
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001921:Brain / Q000166:cytology / Q000502:physiology*; D004594:Electrophysiology / Q000379:methods; D005456:Fluorescent Dyes; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007546:Isoquinolines; D008839:Microelectrodes; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D013779:Tetrodotoxin / Q000494:pharmacology
chemical_list: D005456:Fluorescent Dyes; D007546:Isoquinolines; D013779:Tetrodotoxin; C017475:lucifer yellow