Removal of artifactual bands associated with the presence of 2-mercaptoethanol in two-dimensional polyacrylamide gel electrophoresis.
A method for eliminating artifactual bands due to the presence of 2-mercaptoethanol in two-dimensional gels is described. The method is based on a modification of the procedure of application of the first dimension gel to the SDS slab gel. 2-Mercaptoethanol is removed during equilibration and replaced by iodoacetamide. The use of iodoacetamide improves the recovery of proteins and results in a better detection of them.
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Department of Genetics, Developmental and Molecular Biology, School of Sciences, University of Thessaloniki, Greece.
Lazou
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D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D008565:Membrane Proteins / Q000032:analysis; D008623:Mercaptoethanol / Q000032:analysis* / Q000737:chemistry; D009865:Oocytes / Q000648:ultrastructure; D011895:Rana ridibunda; D012834:Silver; D012967:Sodium Dodecyl Sulfate
D008565:Membrane Proteins; D012967:Sodium Dodecyl Sulfate; D012834:Silver; D008623:Mercaptoethanol