An automated HPLC determination of meticlorpindol in eggs with UV absorbance detection, using on-line dialysis and pre-concentration as sample clean-up; occurrence in and carry over to eggs.
A fully automated HPLC determination of the coccidiostat meticlorpindol in whole egg, egg white and yolk is described. The sample homogenate is dialysed on-line against water. The dialysate is concentrated on-line on a short reversed-phase (RP) column. The contents of this column are transferred to the reversed-phase analytical column by means of the mobile phase. Meticlorpindol is detected using an absorbance detector at 270 nm. Linear calibration graphs are obtained in the range 40-900 ng/g in whole egg and egg white (detection limit 10 ng/g) and 80-1800 ng/g in yolk (detection limit 20 ng/g). Out of 111 commercially obtained egg samples 12 contained meticlorpindol with levels varying from 10 to 433 ng/g. A group of laying hens, kept in cages, received 10 mg/kg of Lerbek (meticlorpindol and methylbenzoquate; Dow Chemical) in the feed for 10 days. Meticlorpindol residues in the eggs rose to a level of 622 ng/g. Meticlorpindol was found in the eggs until 6 days after withdrawal of the medicated feed. Another group received 110 mg/kg in the feed. Meticlorpindol residues rose to levels of 4480 ng/g in the eggs, 5880 ng/g in the egg white and 2660 ng/g in the yolk. Meticlorpindol was found in the eggs and the egg white until 14 days and in the yolk until 8 days after withdrawal of the medicated feed.
Mattern
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Food Inspection Service, Utrecht, The Netherlands.
Kan
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van Gend
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D000821:Animal Feed; D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D003007:Clopidol / Q000008:administration & dosage / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D004529:Egg White / Q000032:analysis; D004530:Egg Yolk / Q000032:analysis; D004531:Eggs / Q000032:analysis*; D005260:Female; D011725:Pyridines / Q000032:analysis*; D012044:Regression Analysis
D011725:Pyridines; D003007:Clopidol