23(S),25(R)-1,25-dihydroxyvitamin D3-26,23-lactone stimulates murine bone formation in vivo.
23(S),25(R)-1,25-Dihydroxyvitamin D3-26,23-lactone (1,25-lactone) has been shown to have unique actions different from those of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3]. In contrast to 1,25-(OH)2D3, 1,25-lactone causes a significant reduction in the serum Ca2+ level, stimulates collagen production in an osteoblastic cell line, and inhibits bone resorption induced by 1,25-(OH)2D3. A possible effect of 1,25-lactone on bone formation was examined in experiments on ectopic bone formation using a bone-inducing factor derived from Dunn osteosarcomas. 1,25-Lactone, a metabolite of 1,25-(OH)2D3, increased [3H]proline uptake at the stage of chondrogenesis and 85Sr uptake during bone formation. Significantly enlarged bone was also induced by this compound 3 weeks after implantation. These results suggest that the 1,25-lactone may be able to stimulate bone formation under in vivo conditions.
Shima
M
M
Department of Pediatrics, Osaka University School of Medicine, Japan.
Tanaka
H
H
Norman
A W
AW
Yamaoka
K
K
Yoshikawa
H
H
Takaoka
K
K
Ishizuka
S
S
Seino
Y
Y
D000818:Animals; D001846:Bone Development / Q000187:drug effects*; D002117:Calcitriol / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D002118:Calcium / Q000097:blood; D003094:Collagen / Q000096:biosynthesis; D008297:Male; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism; D010758:Phosphorus / Q000097:blood; D011392:Proline / Q000378:metabolism; D013326:Strontium Radioisotopes / Q000378:metabolism
D013326:Strontium Radioisotopes; D010758:Phosphorus; C026947:1,25-dihydroxyvitamin D3-26,23-lactone; D003094:Collagen; D011392:Proline; D002117:Calcitriol; D002118:Calcium