Electrofusion of oriented Schizosaccharomyces pombe cells through apical protoplast-protuberances.
The electrofusion of oriented Schizosaccharomyces pombe cells through apical protoplast-protuberances was demonstrated. The protuberances arose after an exposure of early-exponential phase cells to digestive enzymes from hepatopancreas of Helix pomatia. The orientation of cylindric cells within pearl chains was produced by the application of inhomogenous alternating electric fields.
Vondrejs
V
V
Department of Biotechnology, Faculty of Science, Charles University, Prague, Czechoslovakia.
Pavlícek
I
I
Kothera
M
M
Palková
Z
Z
D004558:Electric Stimulation; D008561:Membrane Fusion; D011523:Protoplasts / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D004718:Saccharomycetales / Q000502:physiology*; D012568:Schizosaccharomyces / Q000502:physiology*; D013012:Sorbitol / Q000494:pharmacology
D013012:Sorbitol