Effect of HEPES on the Na+,Cl-dependent uptake of taurine and beta-alanine by cultured glial cells. Modulation by composition and osmolarity of medium.
The effect of HEPES on the Na+,Cl-dependent uptake of taurine and beta-alanine by cultured glial cells was investigated. Whatever the culture medium used, a similar inhibition of the uptake of taurine by HEPES was found. In a previous paper it was shown that this buffer was able to inhibit the uptake of taurine through a slow and a fast "mechanism", when present in the culture or in the incubation medium, respectively. Comparison of the effect of HEPES on the uptake of taurine and beta-alanine suggested that only the slow "mechanism" was common to the uptake of the beta-aminoacid. Therefore, the uptake of the beta-aminoacid might be affected by HEPES. On the other hand, a small increase of the osmolarity of the media altered the uptake of taurine, suggesting that caution is needed when interpreting pharmacological experiments.
Lleu
P L
PL
Centre de Neurochimie du CNRS et Unité 44 de INSERM Strasbourg, France.
Rebel
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D000409:Alanine / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D002645:Chickens; D002712:Chlorides / Q000378:metabolism; D003470:Culture Media; D006531:HEPES / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism*; D009994:Osmolar Concentration; D051381:Rats; D012964:Sodium / Q000502:physiology; D013654:Taurine / Q000378:metabolism*
D002712:Chlorides; D003470:Culture Media; D013654:Taurine; D012964:Sodium; D007930:Leucine; D000409:Alanine; D006531:HEPES