title: Reversal of relative proteinase F activity and onset of androgen-dependent proteinases in the submandibular gland of postnatal mice.
abstract: By isoelectric focusing, we separated trypsin-like proteinases of the mouse submandibular gland (ICR strain) into isozymes with pI values of 4.6 (proteinase F), 5.6 (protease D), 5.8 (protease A), 7.1 and 9.9 (P-esterase). During postnatal development, proteinase F appeared earliest (on the 15th day after birth) and increased in both sexes; however, its percentage ratio to total activity decreased markedly with time because of the rapid increase of other proteinases. On the 22nd day of life, proteinases A and D appeared, and the increase of a proteinase with pI-7.1 followed thereafter. P-esterase was the last isozyme to appear, becoming detectable around 29-45 days. After maturation, the activities of protease A plus D, P-esterase, and the isozyme with a pI value of 7.1 were higher in males than in females, whereas the relative level of proteinase F was reversed. We conclude that proteinase F is appreciably different from the other four proteinases in its development pattern as well as in its responsiveness to sexual hormones.
authors.affiliation: Department of Oral Physiology, Meikai University, School of Dentistry, Saitama, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hosoi
authors.forename: T
authors.initials: T
authors.lastname: Ueha
authors.forename: H
authors.initials: H
authors.lastname: Fukuuchi
authors.forename: M
authors.initials: M
authors.lastname: Kohno
authors.forename: T
authors.initials: T
authors.lastname: Takahashi
mesh_terms: D000728:Androgens / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D016282:Aspartic Acid Endopeptidases / Q000378:metabolism; D001835:Body Weight; D010450:Endopeptidases / Q000378:metabolism*; D004950:Esterases / Q000378:metabolism; D005260:Female; D007526:Isoelectric Point; D007527:Isoenzymes / Q000378:metabolism; D007610:Kallikreins; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009929:Organ Size; D012697:Serine Endopeptidases / Q000378:metabolism*; D012727:Sex Characteristics; D013363:Submandibular Gland / Q000201:enzymology*; D020840:Tissue Kallikreins
chemical_list: D000728:Androgens; D007527:Isoenzymes; D004950:Esterases; D010450:Endopeptidases; D007610:Kallikreins; C489120:Klk13 protein, mouse; D012697:Serine Endopeptidases; C037635:proteinase F; D020840:Tissue Kallikreins; C022325:aspartic proteinase A; D016282:Aspartic Acid Endopeptidases; C489121:Klk1b22 protein, mouse; C489119:Klk9 protein, mouse; C053223:epidermal growth factor binding protein, mouse