Evidence for deficiency of low density lipoprotein receptor on human colonic carcinoma cell lines.
Cells from six human colonic adenocarcinoma lines (CaCo-2, HT29, LS174T, SW480, SW403, and SW1417) and a normal skin fibroblast cell line (AG1519) were assayed in vitro for their ability to use low density lipoprotein (LDL). All tumor cell lines grew well in lipoprotein-deficient serum, implying that LDL in culture medium was not critical for cell growth. When cell growth was inhibited with mevinolin, a cholesterol synthesis inhibitor, the addition of LDL to the medium had no effect on the growth of cells from five of six tumor cell lines. CaCo-2 cells showed a moderate reversal while the fibroblast control showed total reversal of inhibition. A monoclonal antibody to bovine/human LDL receptor, used in an enzyme-linked immunosorbent assay, indicated that only CaCo-2 cells and human skin fibroblast cells consistently demonstrated the presence of LDL receptors. Thus, five of six colon tumor cell lines were unable to overcome a mevinolin block in cholesterol metabolism indicating that these cells were deficient in LDL receptors.
Fabricant
M
M
Department of Microbiology, Boston University School of Medicine, Boston, Massachusetts 02118.
Broitman
S A
SA
D000911:Antibodies, Monoclonal; D002277:Carcinoma / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism; D003110:Colonic Neoplasms / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008148:Lovastatin / Q000494:pharmacology; D011973:Receptors, LDL / Q000172:deficiency*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D008077:Lipoproteins, LDL; D011973:Receptors, LDL; D002784:Cholesterol; D008148:Lovastatin