title: Detection of hippurate hydrolysis by Legionella spp. by using a rapid high-performance liquid chromatography method.
abstract: A rapid high-performance liquid chromatography method was developed to determine hippurate hydrolysis by Legionella spp. Benzoic acid, an end product of enzymatic activity, was directly detected by high-performance liquid chromatography after 1 and 24 h of incubation in 1% sodium hippurate. Because of its sensitivity, this procedure offers more precise identification of some Legionella spp.
authors.affiliation: Laboratoire National de la Santé, Centre National de Référence des Légionelloses, Faculté de Médecine, Lyon, France.
authors.forename: D
authors.initials: D
authors.lastname: Marmet
authors.forename: N
authors.initials: N
authors.lastname: Bornstein
authors.forename: J
authors.initials: J
authors.lastname: Fleurette
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006626:Hippurates / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D007875:Legionella / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D006626:Hippurates; C030514:hippuric acid