title: Quantitative and qualitative tests for urinary luteinizing hormone. Comparison in spontaneous and clomiphene-citrate-treated cycles.
abstract: A study was performed to evaluate conditions in which false-positive and -negative predictions of ovulation occur with qualitative urinary luteinizing hormone (LH) tests. Three urine specimens and a serum sample were collected daily for LH determination from five anovulatory women treated with clomiphene citrate and from six spontaneously cycling women. The urinary LH was determined with one quantitative and three qualitative tests, and the serum LH was determined with a quantitative assay. Ovulation was determined with transvaginal ultrasound and a serum progesterone level on day 22 of the menstrual cycle. There were six ovulatory and five anovulatory cycles. In those 11 cycles the qualitative urinary LH tests were falsely positive twice and falsely negative thrice. All the false-positive and -negative tests except for one occurred when the quantitative urinary LH was 24-28 mIU/mL. Two false-positive tests occurred one to two days after clomiphene administration. Two false-negative tests occurred in a cycle in which follicular development appeared suboptimal, and one occurred in a cycle with a brief urinary LH surge.
authors.affiliation: Department of Obstetrics and Gynecology, University of Chicago, Illinois 60637.
authors.forename: K L
authors.initials: KL
authors.lastname: Ponto
authors.forename: R B
authors.initials: RB
authors.lastname: Barnes
authors.forename: J A
authors.initials: JA
authors.lastname: Holt
mesh_terms: D002996:Clomiphene / Q000008:administration & dosage*; D005188:False Negative Reactions; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D007986:Luteinizing Hormone / Q000097:blood / Q000652:urine*; D010061:Ovulation Detection; D010062:Ovulation Induction / Q000379:methods; D011933:Reagent Kits, Diagnostic
chemical_list: D011933:Reagent Kits, Diagnostic; D002996:Clomiphene; D007986:Luteinizing Hormone