Structure of the amino acid-containing capsular polysaccharide from Escherichia coli O8:K49:H21.
The structure of the capsular antigen of E. coli K49 and the oligosaccharides derived from it by partial acid hydrolysis were studied by 1D- and 2D-n.m.r. spectroscopy, g.l.c.-c.i.-mass spectrometry, and methylation analysis. The K49 polysaccharide consists of the repeating unit----4)-beta-D-GlcpA-(1----6)-beta-D-Galp-(1----6)-beta-D-Glcp- (1----3)-beta - D-GalpNAc-(1----. The glucuronic acid residues are substituted, in the apparent molar ratio of 4:1, with L-threonine and L-serine linked amidically to the carboxyl group.
Beynon
L M
LM
Department of Chemistry, University of British Columbia, Vancouver, Canada.
Dutton
G G
GG
Richards
J C
JC
D000942:Antigens, Bacterial / Q000737:chemistry; D016667:Bacterial Capsules; D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000032:analysis / Q000276:immunology; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000276:immunology
D000942:Antigens, Bacterial; D011135:Polysaccharides, Bacterial; C067064:capsular polysaccharide K49