Inhibition of protein synthesis by acetyl-coenzyme A in a cell-free system: possible involvement of protein acetylation in the regulation of translation.
Acetyl-coenzyme A (CoASAc) inhibits the rate of incorporation of amino acid into protein in a cell-free system of mouse liver. The effect is more pronounced when exogenous mRNA (tobacco mosaic virus or globin mRNA) rather than endogenous messages are used. Micromolar concentrations of the cofactor block initiation, while millimolar concentrations cause a more general inhibition of the translation process, that affects, in addition, the elongation step. Inclusion of [1-14C]acetyl-CoA in a protein synthesis reaction mixture results in a very rapid and selective labelling of a protein of 200 kd of the 'pH 5' fraction. The possible involvement of the acetylating event in the regulation of protein synthesis is discussed.
Nikolakaki
H
H
Laboratory of Biochemistry, School of Chemistry, University of Thessaloniki, Greece.
Giannakouros
T
T
Georgatsos
J G
JG
D000105:Acetyl Coenzyme A / Q000502:physiology*; D000107:Acetylation; D000818:Animals; D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D005470:Fluorometry; D005786:Gene Expression Regulation; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis / Q000502:physiology*; D011506:Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D014027:Tobacco Mosaic Virus / Q000235:genetics
D011506:Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; D000105:Acetyl Coenzyme A