Determination of the packaging capacity of bacteriophage VWB.
VWB is a temperate bacteriophage whose chromosome has cohesive ends. VWB can stably package modified chromosomes that contain insertions of up to about 4 kilobases of foreign DNA. Phage particles containing extra DNA differ from the wild type in their increased sensitivity to chelating agents. Because of these properties, VWB is a promising cloning vector for Streptomyces venezuelae.
Anné
J
J
Department of Microbiology, Rega Institute, Katholieke Universiteit Leuven, Belgium.
Van Mellaert
L
L
Eyssen
H
H
D001435:Bacteriophages / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D015183:Restriction Mapping; D013302:Streptomyces / Q000235:genetics*; D013696:Temperature
D004279:DNA, Viral; D015345:Oligonucleotide Probes