HLA-class II antigens on hemopoietic and stromal cells in human micro long-term bone marrow cultures.
Using a complement-dependent cytotoxicity assay (CDC), we analyzed the presence of HLA-class II antigens on both stromal and hemopoietic cells in a miniaturized human long-term bone marrow culture system. 4-Hydroperoxycyclophosphamide (4-HC)-resistant hemopoietic stem cells capable of restoring in vitro hemopoiesis on irradiated stromal cell layers were HLA-DR, -DP, and -DQ negative. In addition, these cells failed to bind the monoclonal antibody (mAb) Tü 39, previously proposed as a candidate for the recognition of a novel class II antigen, "-DY." On the other hand, the formation of confluent stromal cell layers was inhibited by HLA-DR- or -DP-specific mAbs, but not by the HLA-DQ-specific mAb Tü 22. This suggests the presence of HLA-DR- and/or HLA-DP-positive, but HLA-DQ-negative stromal precursor cells.
Brühl
P
P
GSF Institut für Experimentelle Hämatologie, Munich, FRG.
Mergenthaler
H G
HG
Ellwart
J
J
Dörmer
P
P
D000911:Antibodies, Monoclonal / Q000276:immunology; D001853:Bone Marrow / Q000276:immunology / Q000502:physiology; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003520:Cyclophosphamide / Q000031:analogs & derivatives / Q000276:immunology; D004351:Drug Resistance; D006410:Hematopoiesis / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000276:immunology; D006413:Hematopoietic System / Q000166:cytology* / Q000276:immunology / Q000502:physiology; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D013997:Time Factors
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; D003520:Cyclophosphamide; C011272:perfosfamide