Incidence and reactivity patterns of skeletal and heart (SH) reactive autoantibodies in the sera of patients with myasthenia gravis.
Serum from patients with myasthenia gravis (MG) contain antibodies to numerous skeletal muscle components in addition to the acetylcholine receptor (AChR). Certain non-AChR skeletal muscle autoantibodies have been shown by absorption to cross-react with cardiac muscle, leading to the designation of 'skeletal and heart' or SH antibodies. This study describes a new procedure for the extraction of human cardiac muscle which allows direct determination of SH antibody reactivity. Serologic evaluation of 17 patients with MG revealed 9/17 (53%) were seropositive for SH antibody to cardiac muscle. Absorption of selected MG serum samples with cardiac muscle extracts, reduced or eliminated reactivity to skeletal muscle in all cases, confirming the presence of cross-reactive antibodies. Immunoblot analysis of cardiac muscle extracts demonstrated several distinct antigenic components, which were unrelated to the acetylcholine receptor or to previously identified striational muscle proteins. Serum samples from individual MG patients displayed different immunoblot reactivity patterns ot the antigens in cardiac muscle extracts, providing the first evidence of multiple heart-reactive SH antibodies in MG.
Connor
R I
RI
Department of Medical Microbiology, College of Medicine, Ohio State University, Columbus.
Lefvert
A K
AK
Benes
S C
SC
Lang
R W
RW
D000941:Antigens / Q000276:immunology; D001323:Autoantibodies / Q000032:analysis / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D006982:Hypertonic Solutions; D015151:Immunoblotting; D008297:Male; D008875:Middle Aged; D009124:Muscle Proteins / Q000276:immunology; D009132:Muscles / Q000276:immunology*; D009157:Myasthenia Gravis / Q000097:blood / Q000276:immunology*; D009206:Myocardium / Q000276:immunology*; D011950:Receptors, Cholinergic / Q000276:immunology; D013395:Sucrose
D000941:Antigens; D001323:Autoantibodies; D006982:Hypertonic Solutions; D009124:Muscle Proteins; D011950:Receptors, Cholinergic; D013395:Sucrose