Purification of human cytotrophoblast from term amniochorion by flow cytometry.
Term cytotrophoblast do not express polymorphic MHC Class I antigens, unlike other fetal and maternal cells in the amniochorion/decidua. This allows cytotrophoblast to be isolated and purified from this tissue, utilizing 4E, a monoclonal antibody specific for HLA-B, which labels only non-trophoblast. We have developed a method using enzymic dispersion and Percoll gradient centrifugation, followed by flow cytometry, that yields, on average, a total of 5 X 10(6) term extravillous cytotrophoblast, 97 per cent pure. The availability of highly purified extravillous cytotrophoblast, for the first time, permits precise investigation of trophoblast function.
Shorter
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Harris-Birthright Pre-eclampsia Unit, Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Maternity Department, Headington, Oxford, UK.
Jackson
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Sargent
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Redman
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Starkey
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D000911:Antibodies, Monoclonal; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D010920:Placenta / Q000166:cytology; D014327:Trophoblasts
D000911:Antibodies, Monoclonal