Quantification of radiolabelled granulocyte migration in vivo.
Quantification of the tissue localisation of granulocytes is difficult, particularly in man. With the aim of facilitating such quantification, a technique is described which combines the micropore filter skin window technique with the infusion of autologous 111In-labelled granulocytes. The radioactivity in the removable micropore filters, placed on small forearm skin window abrasions, was compared with peripheral blood radioactivity following injection of 111In-labelled 'pure' granulocytes in normal subjects. Radioactivity in filters from abrasions which were made 8 h or more before injection of labelled cells followed the same time course as the cell-associated radioactivity in whole blood, but radioactivity in filters from abrasions made nearer to the time of labelled granulocyte injection increased to reach a peak 3-8 h after cell injection, at a time when cell-associated blood radioactivity had fallen to about 50% of its initial value. Exercise appeared to induce a transient decrease in radiolabelled granulocyte migration into the filters. This technique offers a means of studying the kinetics of granulocyte migration in vivo.
Pinching
A J
AJ
Department of Medicine, Hammersmith Hospital, London, U.K.
Peters
A M
AM
Saverymuttu
S H
SH
D002465:Cell Movement; D005374:Filtration; D006098:Granulocytes / Q000276:immunology*; D006801:Humans; D007205:Indium Radioisotopes; D008297:Male; D012867:Skin / Q000098:blood supply
D007205:Indium Radioisotopes