Murine c-rel transcription is rapidly induced in T-cells and fibroblasts by mitogenic agents and the phorbol ester 12-O-tetradecanoylphorbol-13-acetate.
The c-rel protooncogene is here shown to be a member of the early response gene family. Expression induced by different agents is regulated by both transcriptional and posttranscriptional mechanisms. In quiescent fibroblasts, c-rel expression is maximally induced by serum or 12-O-tetradecanoylphorbol-13-acetate within 60 min and is superinduced in serum-stimulated fibroblasts by cycloheximide. In T-cells, although 12-O-tetradecanoylphorbol-13-acetate and concanavalin A both rapidly activate c-rel expression, the kinetics of induction mediated by these agents differs markedly. Nuclear run-on analysis demonstrates that induced c-rel expression is due primarily to increased transcription, and the rapid decrease in expression observed in serum- and 12-O-tetradecanoylphorbol-13-acetate-stimulated cells results from mRNA turnover. In the B-lymphoid lineage, c-rel is constitutively transcribed, with the differentiation stage-specific decrease in c-rel expression seen in plasmacytomas reflecting posttranscriptional regulation.
Grumont
R J
RJ
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Parkville, Victoria, Australia.
Gerondakis
S
S
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D001790:Blood Physiological Phenomena; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D051379:Mice; D008934:Mitogens / Q000494:pharmacology*; D011519:Proto-Oncogenes / Q000187:drug effects*; D013601:T-Lymphocytes / Q000187:drug effects*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects*
D008934:Mitogens; D003208:Concanavalin A; D003513:Cycloheximide; D013755:Tetradecanoylphorbol Acetate