title: Elevated natural killer cell responses in mice infected with recombinant vaccinia virus encoding murine IL-2.
abstract: The role of cytotoxic T cells and NK cells in the recovery of immunodeficient, athymic, nude mice infected with a recombinant vaccinia virus (VV) encoding murine IL-2 was investigated. Kinetic studies with the IL-2-encoding recombinant (VV-HA-IL2) and control (VV-HA-TK) viruses excluded a role for cytotoxic T cells but suggested the possible involvement of NK cells. In athymic nude mice given VV-HA-IL2, NK activity was at least threefold higher than mice infected with VV-HA-TK and this activity persisted for at least 6 days after infection. The effectors mediating the NK-like activity were asialo-GM1+ (as-GM1+), Thy1.2+/-, CD4- and CD8-, the phenotype of conventional NK cells. Elevated NK activity coincided with the rapid clearance of VV-HA-IL2 from ovaries of infected normal CBA/H mice but not from ovaries of CBA beige mice which had no detectable NK activity in spleens or ovaries. The expression of IL-2 in recombinant VV infection probably induces a cascade of immunologic effects of which elevated NK activity is one. We speculate that the chemoattractant and NK activity augmenting effects of IL-2 may contribute to recovery from VV-infection.
authors.affiliation: Division of Cell Biology, John Curtin School of Medical Research, Australian National University, Canberra.
authors.forename: G
authors.initials: G
authors.lastname: Karupiah
authors.forename: B E
authors.initials: BE
authors.lastname: Coupar
authors.forename: M E
authors.initials: ME
authors.lastname: Andrew
authors.forename: D B
authors.initials: DB
authors.lastname: Boyle
authors.forename: S M
authors.initials: SM
authors.lastname: Phillips
authors.forename: A
authors.initials: A
authors.lastname: Müllbacher
authors.forename: R V
authors.initials: RV
authors.lastname: Blanden
authors.forename: I A
authors.initials: IA
authors.lastname: Ramshaw
mesh_terms: D000818:Animals; D003602:Cytotoxicity, Immunologic; D004274:DNA, Recombinant; D005260:Female; D007111:Immunity, Cellular; D007376:Interleukin-2 / Q000502:physiology*; D007694:Killer Cells, Natural / Q000276:immunology*; D008099:Liver / Q000382:microbiology / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D010053:Ovary / Q000276:immunology / Q000473:pathology; D013154:Spleen / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014615:Vaccinia / Q000276:immunology*; D014616:Vaccinia virus; D014779:Virus Replication
chemical_list: D004274:DNA, Recombinant; D007376:Interleukin-2