title: Stability of brain intracellular lactate and 31P-metabolite levels at reduced intracellular pH during prolonged hypercapnia in rats.
abstract: The tolerance of low intracellular pH (pHi) was examined in vivo in rats by imposing severe, prolonged respiratory acidosis. Rats were intubated and ventilated for 10 min with 20% CO2, for 75 min with 50% CO2, and for 10 min with 20% CO2. The maximum PaCO2 was 320 mm Hg. Cerebral intracellular lactate, pHi, and high-energy phosphate metabolites were monitored in vivo with 31P and 1H nuclear magnetic resonance (NMR) spectroscopy, using a 4.7-T horizontal instrument. Within 6 min after the administration of 50% CO2, pHi fell by 0.57 +/- 0.03 unit, phosphocreatine decreased by approximately 20%, and Pi increased by approximately 100%. These values were stable throughout the remainder of the hypercapnic period. Cerebral intracellular lactate, visible with 1H NMR spectroscopy in the hyperoxic state, decreased during hypercapnia, suggesting either a favorable change in oxygen availability (decreased lactate production) or an increase in lactate clearance or both. All hypercapnic animals awakened and behaved normally after CO2 was discontinued. Histological examination of cortical and hippocampal areas, prepared using a hematoxylin and eosin stain, showed no areas of necrosis and no glial infiltrates. However, isolated, scattered, dark-staining, shrunken neurons were detected both in control animals (no exposure to hypercapnia) and in animals that had been hypercapnic. This subtle histological change could represent an artifact resulting from imperfect perfusion-fixation, or it could represent subtle neurologic injury during the hypercapnia protocol. In summary, extreme hypercapnia and low pHi (approximately 6.5) are well tolerated in rats for periods up to 75 min if adequate oxygenation is maintained.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Anesthesia, University of California, San Francisco 94143.
authors.forename: Y
authors.initials: Y
authors.lastname: Cohen
authors.forename: L H
authors.initials: LH
authors.lastname: Chang
authors.forename: L
authors.initials: L
authors.lastname: Litt
authors.forename: F
authors.initials: F
authors.lastname: Kim
authors.forename: J W
authors.initials: JW
authors.lastname: Severinghaus
authors.forename: P R
authors.initials: PR
authors.lastname: Weinstein
authors.forename: R L
authors.initials: RL
authors.lastname: Davis
authors.forename: I
authors.initials: I
authors.lastname: Germano
authors.forename: T L
authors.initials: TL
authors.lastname: James
mesh_terms: D000142:Acidosis, Respiratory / Q000209:etiology / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D006859:Hydrogen; D006935:Hypercapnia / Q000150:complications / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D010710:Phosphates / Q000378:metabolism*; D010758:Phosphorus; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007773:Lactates; D010710:Phosphates; D010758:Phosphorus; D006859:Hydrogen