Fresh versus cryopreserved cultured allografts for the treatment of chronic skin ulcers.
Both fresh and cryopreserved cultured epithelial allografts were used to treat 20 patients with a total of 30 chronic ulcers. A profound stimulation of host epithelialization from the wound edges and epidermal appendages was observed. Our data suggest that allografts, prepared using a simple cryopreservation technique, retain their morphological and functional characteristics, and are as effective as fresh allografts in healing chronic ulceration.
Teepe
R G
RG
Department of Dermatology, University Hospital Leiden, The Netherlands.
Koebrugge
E J
EJ
Ponec
M
M
Vermeer
B J
BJ
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002908:Chronic Disease; D015925:Cryopreservation; D046508:Culture Techniques; D005260:Female; D006801:Humans; D015603:Keratinocytes / Q000637:transplantation*; D008297:Male; D008875:Middle Aged; D012883:Skin Ulcer / Q000601:surgery*; D013997:Time Factors; D014184:Transplantation, Homologous; D014945:Wound Healing / Q000502:physiology