title: Interaction of interleukin-5 with its receptors on murine leukemic BCL1 cells and its implication in biological activity.
abstract: Interaction of interleukin (IL)-5 with its receptors on murine leukemic cell line, BCL1 cells was examined. 125I-labeled recombinant murine IL-5(rmIL-5) bound specifically to high-affinity receptors on BCL1 cells. rmIL-5, which was about 2500-fold more active than recombinant human IL-5(rhIL-5) in IgM-inducing activity on BCL1 cells, also showed about 5000-fold higher affinity to receptors. These results suggest that the bioactivity of IL-5 correlates with its receptor-binding activity. When disulfide bond formation was blocked, rmIL-5 dissociated into a monomer and lost its biological activity. This monomeric form of rmIL-5 also lost its ability to bind to cells, suggesting that dimer formation is essential for the biological activity of IL-5.
authors.affiliation: Suntory Institute for Biomedical Research, Mishima, Osaka, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Tsuruoka
authors.forename: K
authors.initials: K
authors.lastname: Funakoshi
authors.forename: S
authors.initials: S
authors.lastname: Kodama
authors.forename: M
authors.initials: M
authors.lastname: Tsujimoto
mesh_terms: D000818:Animals; D005033:Ethylmaleimide / Q000494:pharmacology; D015848:Interleukin-5 / Q000378:metabolism* / Q000494:pharmacology; D007457:Iodine Radioisotopes; D007942:Leukemia, Experimental / Q000378:metabolism*; D051379:Mice; D011971:Receptors, Immunologic / Q000378:metabolism*; D018123:Receptors, Interleukin; D053649:Receptors, Interleukin-5; D014407:Tumor Cells, Cultured
chemical_list: D015848:Interleukin-5; D007457:Iodine Radioisotopes; D011971:Receptors, Immunologic; D018123:Receptors, Interleukin; D053649:Receptors, Interleukin-5; D005033:Ethylmaleimide