Drosophila glutathione S-transferase 1-1 shares a region of sequence homology with the maize glutathione S-transferase III.
We have characterized a Drosophila glutathione S-transferase (RX:glutathione R-transferase, EC 2.5.1.18) cDNA encoding a protein of 209 amino acids. The cDNA was expressed in Escherichia coli harboring the expression plasmid construct pGTDml-KK. The active enzyme, designated as Drosophila glutathione S-transferase 1-1, had a specific activity toward 1-chloro-2,4-dinitrobenzene comparable to that for the mammalian glutathione S-transferases but did not have as broad a substrate specificity pattern. There is a region of 44 amino acids in this enzyme that shares 66% identity with an analogous region of maize glutathione S-transferase III. Drosophila glutathione S-transferase 1-1 had no obvious homology to any mammalian or parasitic glutathione S-transferases. The gene was found to be a member of a multigene family.
Toung
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YP
Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.
Hsieh
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Tu
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004330:Drosophila / Q000196:embryology / Q000201:enzymology / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000201:enzymology; D005796:Genes; D015698:Genomic Library; D005982:Glutathione Transferase / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D003313:Zea mays / Q000201:enzymology / Q000235:genetics
D007527:Isoenzymes; D005982:Glutathione Transferase