L-carnitine metabolization and osmotic stress response in Escherichia coli.
Growth of Escherichia coli 044 K 74 in liquid medium of raised osmotic strength was stimulated by exogenous L-carnitine, crotonobetaine and gamma-butyrobetaine, respectively. L-Carnitine was accumulated within the cells in dependence on the salt concentration of the media. Osmotic stress during aerobic or anaerobic growth with glucose triggered the L-carnitine uptake in E. coli 044 K 74 whereas L-carnitine uptake by cells of this organism grown anaerobically on glycerol/fumarate was only slightly modified. Synthesis of the enzymes metabolizing L-carnitine to gamma-butyrobetaine in glycerol/fumarate growing bacteria was found to be completely repressed by high NaCl-concentrations. Together, these results indicate that most likely the L-carnitine metabolization sequence does not play a role in osmoregulation in E. coli 044 K 74.
Jung
H
H
Berich Biochemie, Karl-Marx-Universität Leipzig, DDR.
Jung
K
K
Kleber
H P
HP
D000217:Acyltransferases; D002331:Carnitine / Q000378:metabolism*; D003470:Culture Media; D004790:Enzyme Induction / Q000187:drug effects; D004926:Escherichia coli / Q000378:metabolism* / Q000502:physiology; D006836:Hydro-Lyases / Q000096:biosynthesis; D009097:Multienzyme Complexes; D009994:Osmolar Concentration; D010088:Oxidoreductases / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D014882:Water-Electrolyte Balance
D003470:Culture Media; D009097:Multienzyme Complexes; D012965:Sodium Chloride; D010088:Oxidoreductases; C067153:crotonobetaine reductase; D000217:Acyltransferases; C060278:crotonobetainyl-CoA carnitine coenzyme A transferase; D006836:Hydro-Lyases; D002331:Carnitine