title: Neural nicotinic acetylcholine responses in sensory neurons from postnatal rat.
abstract: The whole-cell configuration of the patch-clamp technique was used to study nicotinic acetylcholine (ACh) responses in freshly dissociated dorsal root ganglion (DRG) cells from postnatal rat. At negative holding potentials with physiological solutions in the bath and the pipette, ACh (20 microM), nicotine (5 microM) or DMPP (20 microM) activated inward currents in 51% of the cells. Average current density was higher in 1-month-old compared to newborn animals. Nicotinic agonist-induced currents were unaffected by atropine (10 microM) but reversibly blocked by hexamethonium (20 microM). Although labeling with fluorescent alpha-bungarotoxin (BGT) demonstrated the presence of toxin binding sites on DRG cells, DMPP-induced inward currents were unaffected by micromolar BGT. Neuronal bungarotoxin (100 nM), in contrast, led to a largely irreversible block of the nicotinic responses. These results show that postnatal DRG cells express functional nicotinic acetylcholine receptors (nAChR) of a neuronal type.
authors.affiliation: Department of Neurology, Children's Hospital, Boston, MA 02115.
authors.forename: N J
authors.initials: NJ
authors.lastname: Sucher
authors.forename: T P
authors.initials: TP
authors.lastname: Cheng
authors.forename: S A
authors.initials: SA
authors.lastname: Lipton
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002038:Bungarotoxins; D002478:Cells, Cultured; D005456:Fluorescent Dyes; D005727:Ganglia, Spinal / Q000187:drug effects*; D008564:Membrane Potentials / Q000187:drug effects; D009475:Neurons, Afferent / Q000187:drug effects*; D011978:Receptors, Nicotinic / Q000187:drug effects*
chemical_list: D002038:Bungarotoxins; D005456:Fluorescent Dyes; D011978:Receptors, Nicotinic