Mass concentration measurements of creatine kinase BB isoenzyme as an index of brain tissue damage.
A new two-site immunoenzymometric method using monoclonal antibodies was developed for measuring CK-BB mass concentrations in cerebrospinal fluid (CSF). Within- and between-assay coefficient of variation values for the method varied between 6 and 9%. Assay results are not affected by presence of sulfate and sialic acid groups on the enzyme. In comparison to catalytic activity measurements, a steady decline in the enzyme's specific activity was observed after acute head trauma. Repetitive measurements of CK-BB mass concentration in cerebrospinal fluid during the first 24 h after trauma enabled the estimation of brain lesion size. Clinical outcome of acute head trauma patients evaluated by Glasglow Outcome Scale, correlated well with cumulative CK-BB release after trauma. Also in neonates, CK-BB determinations in CSF correlated well with clinical findings.
Delanghe
J R
JR
Department of Clinical Chemistry, University Hospital, Gent, Belgium.
De Winter
H A
HA
De Buyzere
M L
ML
Camaert
J J
JJ
Martens
F E
FE
De Praeter
C
C
D000293:Adolescent; D000328:Adult; D001921:Brain / Q000201:enzymology; D001930:Brain Injuries / Q000175:diagnosis*; D004796:Clinical Enzyme Tests; D003402:Creatine Kinase / Q000134:cerebrospinal fluid*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007527:Isoenzymes; D008297:Male; D008875:Middle Aged
D007527:Isoenzymes; D003402:Creatine Kinase