title: Associations between beta-tubulin and mitochondria in adult isolated heart myocytes as shown by immunofluorescence and immunoelectron microscopy.
abstract: We have investigated the associations between beta-tubulin and mitochondria in freshly isolated cardiac myocytes from the rat. Beta-tubulin was identified by using monoclonal antibodies for immunofluorescence and high resolution immunogold electron microscopy. In addition, conventional transmission and scanning electron microscopic studies were performed. After chemical stabilization in a formaldehyde solution, the myocytes were shock-frozen at -150 degrees C, cryosectioned at -70 degrees C and subsequently processed for immunohistochemical and immunocytochemical microscopy. A characteristic of the rod shaped myocytes is the presence of a dense network of microtubules in the cytoplasm displaying a pattern of strong anti-beta-tubulin reaction. The complexity of this network however varies considerably among the myocytes reflecting microtubule dynamic instability. Further, our findings demonstrate that the beta-tubulin label in rod cells is confined to the perinuclear and interfibrillar spaces and, therefore, is largely colocalized with the cytoplasmic organelles. In myocytes undergoing severe contracture the distribution of beta-tubulin is entirely restricted to the outer mitochondrial-containing domain. This implies that, in a cell model with marked segregation of the contractile filaments and organelles, mitochondria are codistributed with microtubules in the total absence of desmin intermediate filaments. Moreover, our immunogold preparations demonstrate anti-beta-tubulin labelling in the outer mitochondrial membrane as well as of fibres in close apposition to this membrane. These results indicate the presence of a specific beta-tubulin binding to the outer mitochondrial membrane that probably also involves microtubule based translocators and/or MAPs.
authors.affiliation: Department of Anatomy, University of Bergen, Norway.
authors.forename: T
authors.initials: T
authors.lastname: Saetersdal
authors.forename: G
authors.initials: G
authors.lastname: Greve
authors.forename: H
authors.initials: H
authors.lastname: Dalen
mesh_terms: D000818:Animals; D005260:Female; D005455:Fluorescent Antibody Technique; D008855:Microscopy, Electron, Scanning; D016253:Microscopy, Immunoelectron; D008870:Microtubules / Q000737:chemistry / Q000648:ultrastructure*; D008929:Mitochondria, Heart / Q000737:chemistry / Q000648:ultrastructure*; D009206:Myocardium / Q000737:chemistry / Q000648:ultrastructure*; D051381:Rats; D011919:Rats, Inbred Strains; D014404:Tubulin / Q000032:analysis*
chemical_list: D014404:Tubulin