Estrogen metabolism in the (New Zealand black x New Zealand white)F1 murine model of systemic lupus erythematosus.
Hepatic microsomal estrogen metabolism was analyzed in the (New Zealand black x New Zealand white)F1 ([NZB x NZW]F1) murine model of systemic lupus erythematosus. Both the estrogen 2-hydroxylase activity (per mg microsomal protein) and the hepatic cytochrome P-450 content were higher in premorbid (NZB x NZW)F1 mice, as compared with similarly aged nonautoimmune mice. However, these differences were not associated with alterations in the relative formation of the 2-hydroxylated and the 16 alpha-hydroxylated metabolites. The development of overt nephritis was associated with a decrease in estrogen metabolic activity, but not with any alteration in the distribution of estrogen metabolites. Thus, estrogen metabolism was not altered in premorbid (NZB x NZW)F1 mice in a manner that would result in abnormal retention of hormonally active metabolites.
Baer
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Department of Medicine, State University of New York, Buffalo 14215.
Green
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D000818:Animals; D019363:Cytochrome P-450 CYP1A1; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004967:Estrogens / Q000378:metabolism* / Q000493:pharmacokinetics; D004970:Estrone / Q000378:metabolism; D006900:Hydroxylation; D008180:Lupus Erythematosus, Systemic / Q000378:metabolism*; D008181:Lupus Nephritis / Q000378:metabolism; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012741:Sexual Maturation; D013250:Steroid Hydroxylases / Q000378:metabolism
D004967:Estrogens; D004970:Estrone; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D019363:Cytochrome P-450 CYP1A1; C020120:estrogen 2-hydroxylase