title: Effects of restraint stress on catecholamine concentrations in the glandular stomach of rats.
abstract: Restraint stress is known to induce gastric ulcers in rats. Peripheral sympathetic activity and catecholamines are involved in the pathogenesis of these gastric ulcers. The aim of the present study was to evaluate the effects of restraint on mucosal and muscle catecholamine concentrations in the glandular stomach of rats. In unrestrained rats, noradrenaline concentration was higher in the muscle than in the mucosa of the glandular stomach (629 +/- 106 vs 18 +/- 3 pg/mg and 217 +/- 37 vs 18 +/- 8 pg/mg, respectively in the corpus and the antrum, p less than 0.01). This can be explained by the existence of an abundant noradrenergic innervation in the muscle layer. After 20 hours of restraint, adrenaline and noradrenaline concentrations were significantly decreased in adrenals, in comparison with unrestrained animals (255 +/- 53 vs 638 +/- 160 ng/mg and 113 +/- 17 vs 198 +/- 37 ng/mg, respectively for adrenaline and noradrenaline, p less than 0.05). In the glandular stomach, noradrenaline and adrenaline concentrations in restrained rats were not significantly different from those in unrestrained rats. However, adrenaline concentrations in the muscle of restrained rats were higher than in the mucosa. Moreover, restraint induced a significant decrease in dopamine concentration in the antral mucosa (from 100 +/- 12 pg/mg in unrestrained rats to 15 +/- 5 pg/mg in restrained rats), suggesting that a depletion in dopamine in the antral mucosa could be one of the pathogenetic factors involved in antral gastric stress-induced ulcers in rats.
authors.affiliation: Laboratoire de Pharmacologie Clinique Faculté de Médecine Broussais-Hôtel-Dieu, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Gaudin
authors.forename: M
authors.initials: M
authors.lastname: Safar
authors.forename: J L
authors.initials: JL
authors.lastname: Cuche
mesh_terms: D000818:Animals; D004298:Dopamine / Q000378:metabolism*; D004837:Epinephrine / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism*; D008297:Male; D009638:Norepinephrine / Q000378:metabolism*; D011706:Pyloric Antrum / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012149:Restraint, Physical; D013312:Stress, Physiological / Q000378:metabolism*
chemical_list: D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine