title: [Site-specific endonucleases LplI and AagI].
abstract: New site-specific endonucleases LplI and AagI have been isolated from the Lactobacillus plantarum and Achromobacter agile cells, respectively. The enzymes' purification stages included treatment of cell-free extracts with polyethylenimine, fractionation in two-phase system by Albertsson's method, chromatography on blue Sepharose and DEAE-cellulose. The results of cleavage of a 5'-32P-labelled oligodeoxynucleotide duplex by restriction endonucleases LplI and AagI indicate that these enzymes recognize and cut the sequence AT decreases CGAT, being therefore true isoschizomers of the ClaI restriction endonuclease from Caryophanon latum. The L. plantarum strain has 400 fold endonuclease productivity as compared with the ClaI producent and is perspective for preparative isolation of LplI.
authors.forename: N N
authors.initials: NN
authors.lastname: Sokolov
authors.forename: Z P
authors.initials: ZP
authors.lastname: Manialene
authors.forename: V V
authors.initials: VV
authors.lastname: Butkus
authors.forename: A B
authors.initials: AB
authors.lastname: Fitsner
authors.forename: E B
authors.initials: EB
authors.lastname: Khoroshutina
authors.forename: A A
authors.initials: AA
authors.lastname: Kalugin
authors.forename: A A
authors.initials: AA
authors.lastname: Ianulaĭtis
mesh_terms: D000421:Alcaligenes / Q000201:enzymology; D001483:Base Sequence; D004247:DNA / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D007778:Lactobacillus / Q000201:enzymology; D008969:Molecular Sequence Data
chemical_list: D004247:DNA; C032824:ATCGAT-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific
vernacular_title: Saĭt-spetsificheskie éndonukleazy LplI i AagI.