title: [A study of hexokinase isoenzymes in rat sarcoma M-1].
abstract: Using a wide spectrum of criteria, the isozyme composition of hexokinase from sarcoma M-1 reinoculated to rat m. gastrocnemius was studied. The structural, physicochemical and functional properties of the homogeneous enzyme which is represented in sarcoma M-1 by one molecular form, were investigated. Some properties of the enzyme (amino acid composition, resistance to mild proteolysis, Mr, pH-dependence of enzyme activity, electrophoretic mobility, kinetic behaviour) indicate that sarcoma M-1 hexokinase is a specific form of the enzyme which differs markedly from other known isozymes of mammalian hexokinase. The observed peculiarities of sarcoma M-1 hexokinase are discussed in terms of present-day concepts on the structure of isozymic spectra of enzymes in neoplastic tissues.
authors.forename: N F
authors.initials: NF
authors.lastname: Garkusha
authors.forename: N Iu
authors.initials: NIu
authors.lastname: Goncharova
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D001667:Binding, Competitive; D004591:Electrophoresis, Polyacrylamide Gel; D006593:Hexokinase / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008970:Molecular Weight; D051381:Rats; D012513:Sarcoma, Experimental / Q000201:enzymology* / Q000378:metabolism
chemical_list: D000596:Amino Acids; D007527:Isoenzymes; D006593:Hexokinase
vernacular_title: Issledovanie izozimnogo sostava geksokinazy v sarkome M-1 krys.