New thymocyte growth factor from thymic epithelial cell line.
Three polypeptide fractions were separated from the culture supernatant of a thymic epithelial cell line, TAD3, by high-pressure liquid chromatography (HPLC) equipped with gel-filtration column (GFC). One (estimated molecular weight: 10 kD) of the polypeptide fractions possessed the capacity to induce thymocyte proliferation. The sensitive cells for the growth factor in the fraction seem to be immature thymocytes which exist in the outer-cortical or the subcapsular area of thymic lobule. Furthermore, the mechanism to proliferate the thymocytes appears to differ from that of other cytokines. Thus, the fraction might possibly contain a previously unidentified thymocyte growth factor.
Kinoshita
Y
Y
Department of Physiology, Osaka City University Medical School, Japan.
Toyokawa
T
T
Hato
F
F
Masuda
A
A
Matsuyama
M
M
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004847:Epithelial Cells; D004848:Epithelium / Q000737:chemistry; D006133:Growth Substances / Q000302:isolation & purification* / Q000494:pharmacology; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects; D013950:Thymus Gland / Q000737:chemistry* / Q000166:cytology
D006133:Growth Substances; D010455:Peptides