title: Effects of (3-amino-1-hydroxypropylidene)-1,1-bisphosphonate on mouse osteoclasts.
abstract: A group of 5-day-old mice were injected intraperitoneally with (3-amino-1-hydroxypropylidine)-1,1-bisphosphonate (APD). Morphologic changes were observed in vitally stained osteoclasts on parietal bones 3 days later, and these were judged to be degenerative. At this time significantly increased numbers of nuclei per osteoclast and total numbers of osteoclast nuclei were observed. However, at 4 days after the injection of APD, the total numbers of osteoclasts were significantly reduced relative to controls. When parietal bones were maintained in culture, APD reduced osteoclast numbers and inhibited cell-mediated 45Ca2+ release. Exposure of bones to parathyroid hormone increased the number of osteoclasts counted 1 day later. This effect was not blocked by APD. Calcitonin prevented the reduction in osteoclast numbers due to APD in vitro. We conclude that APD has a direct effect on resorbing mouse osteoclasts.
authors.affiliation: Charles Salt Research Centre, Robert Jones and Agnes Hunt Orthopaedic Hospital, Oswestry, Shropshire, England.
authors.forename: M J
authors.initials: MJ
authors.lastname: Marshall
authors.forename: A S
authors.initials: AS
authors.lastname: Wilson
authors.forename: M W
authors.initials: MW
authors.lastname: Davie
mesh_terms: D000818:Animals; D002116:Calcitonin / Q000494:pharmacology; D002132:Calcium Radioisotopes; D002250:Carbon Radioisotopes; D002452:Cell Count / Q000187:drug effects; D004164:Diphosphonates / Q000494:pharmacology*; D066298:In Vitro Techniques; D051379:Mice; D008808:Mice, Inbred CBA; D010010:Osteoclasts / Q000187:drug effects*; D000077268:Pamidronate; D010281:Parathyroid Hormone / Q000494:pharmacology
chemical_list: D002132:Calcium Radioisotopes; D002250:Carbon Radioisotopes; D004164:Diphosphonates; D010281:Parathyroid Hormone; D002116:Calcitonin; D000077268:Pamidronate