Expression of photosynthesis-related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts.
We have analyzed the expression of chimeric genes in populations of protoplasts isolated from the photosynthetic and nonphotosynthetic tissues within leaves of transgenic tobacco plants and separated by fluorescence-activated cell sorting. Expression of transcriptional gene fusions controlled by promoters from photosynthesis-associated genes showed a striking dependence on cell type. These patterns of expression were preserved when the gene fusions were transfected into normal (nontransgenic) tobacco leaf protoplasts.
Harkins
K R
KR
Department of Plant Sciences, University of Arizona, Tucson 85721.
Jefferson
R A
RA
Kavanagh
T A
TA
Bevan
M W
MW
Galbraith
D W
DW
D001483:Base Sequence; D002678:Chimera; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects; D005796:Genes; D006540:Herbicides / Q000494:pharmacology; D008969:Molecular Sequence Data; D009029:Mosaic Viruses; D010788:Photosynthesis; D010944:Plants / Q000235:genetics*; D010947:Plants, Toxic; D011523:Protoplasts / Q000378:metabolism; D011724:Pyridazines / Q000494:pharmacology; D014026:Nicotiana / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection
D006540:Herbicides; D011724:Pyridazines; D004247:DNA; C008677:norflurazone