title: Nerve impulse-induced release of endogenous noradrenaline and adrenaline from the perfused cod spleen.
abstract: Release of endogenous catecholamines (CA) by electrical nerve stimulation (NS) was studied in the isolated perfused spleen of the Atlantic cod, Gadus morhua. An HPLC-system for the analysis of endogenously released CA is described. Cocaine (COC) was used to block neuronal re-uptake of endogenous CA released by NS. Splanchnic NS at frequencies of 1-40 Hz for 20 s resulted in release of noradrenaline (NA) and adrenaline (A) with a maximal total overflow at 20 Hz for both amines. The release of CA was frequency-dependent. COC (0.1 mmol.l-1) increased NS-evoked (40 Hz) overflow of NA and A by 4.8 and 2.2 times, respectively. and reduced the overflow of dihydroxyphenylglycol (DOPEG) to spontaneous efflux levels or less. It can be concluded that the HPLC-technique used was adequate for measurement of NS-evoked release of endogenous CA and DOPEG from the isolated perfused cod spleen, and the model presented can therefore be used when studying adrenergic mechanisms in fish spleen.
authors.affiliation: Department of Zoophysiology, University of Göteborg, Sweden.
authors.forename: F
authors.initials: F
authors.lastname: Ehrenström
authors.forename: A L
authors.initials: AL
authors.lastname: Ungell
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D003042:Cocaine / Q000494:pharmacology; D004558:Electric Stimulation; D004837:Epinephrine / Q000378:metabolism*; D005260:Female; D005399:Fishes / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D009638:Norepinephrine / Q000378:metabolism*; D010477:Perfusion; D013154:Spleen / Q000187:drug effects / Q000294:innervation / Q000378:metabolism*
chemical_list: D003042:Cocaine; D002118:Calcium; D009638:Norepinephrine; D004837:Epinephrine