title: [Comparative analysis of 2 methods of DNA extraction].
abstract: The study of the organization of immunoglobulin and T cell receptor genes in leukaemias and lymphomas depends on our ability to obtain high molecular weight DNA. We have compared two different methods of DNA extraction: one being enzymatic, using the enzyme proteinase K, and the other chemical, using urea as a substance purifying DNA. Since the amount and purity of the DNA obtained are similar with each of the two methods of DNA extraction, both are valid to make such a genetic analysis.
authors.affiliation: Departamento de Medicina, Hospital Clínico Universitario, Salamanca.
authors.forename: I
authors.initials: I
authors.lastname: Sánchez García
authors.forename: C
authors.initials: C
authors.lastname: Martín Seisdedos
authors.forename: R
authors.initials: R
authors.lastname: Pérez Grande
authors.forename: J
authors.initials: J
authors.lastname: Corral de la Calle
authors.forename: C
authors.initials: C
authors.lastname: Rodríguez Arias
authors.forename: R
authors.initials: R
authors.lastname: González-Sarmiento
mesh_terms: D011232:Chemical Precipitation; D004273:DNA, Neoplasm / Q000302:isolation & purification*; D019286:Endopeptidase K; D006801:Humans; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000473:pathology; D008970:Molecular Weight; D012697:Serine Endopeptidases; D014407:Tumor Cells, Cultured / Q000737:chemistry; D014508:Urea
chemical_list: D004273:DNA, Neoplasm; D014508:Urea; D012697:Serine Endopeptidases; D019286:Endopeptidase K
vernacular_title: Análisis comparativo de dos métodos de extracción de ADN.