title: Histologic, morphometric, and biochemical evolution of vein bypass grafts in a nonhuman primate model. I. Sequential changes within the first three months.
abstract: The objective of this study was to define the histologic and morphometric evolution that accompanies the increase in cholesterol content of vein bypass grafts in a nonhuman primate model. Cephalic vein grafts were interposed bilaterally in the femoral arteries of 15 stump-tailed macaque monkeys (Macaca arctoides), which were fed a diet that sustains plasma cholesterol levels of approximately 225 mg/dl. Grafts were excised from five animals for analysis on each of postoperative days 3, 7, 14, 30, 60, and 90. Cholesterol content increased from 69 +/- 24 micrograms/100 mg (mean +/- standard deviation) in ungrafted vein to 473 +/- 122 micrograms/100 mg in grafts 90 days after implantation (p less than 0.05). By stepwise regression analysis, cholesterol content was best predicted by abundance of foam cells (r2 = 0.82). Intima comprised 13% +/- 5% of the total cross-sectional area of the wall in ungrafted vein and 59% +/- 11% at day 90 (p less than 0.001). With cholesterol content excluded from the stepwise regression, intimal area was best predicted by the presence of foam cells (r2 = 0.39). There was consistently an increase in the prevalence of polymorphonuclear leukocytes on the luminal surface and in both the intima and media during the first 14 days after grafting. Vasa vasorum, which were always present in ungrafted vein, were sparse at 3 days but reappeared by day 7. Medial fibrosis occurred in grafts, and in the 30- to 90-day interval it was directly correlated with the number of adventitial vasa vasorum present (r = 0.64, p less than 0.05). Immunohistochemistry revealed prominent staining for both platelet factor VIII and fibronectin during the first month, with a gradual decline in staining intensity thereafter. The evolution of changes in vein bypass grafts documented in this report are in general agreement with graft changes observed in humans and support the validity of our model in evaluating the histologic correlates of increased graft cholesterol content.
authors.affiliation: Department of Cardiothoracic Surgery, Medical College of Wisonsin, Milwaukee.
authors.forename: L E
authors.initials: LE
authors.lastname: Boerboom
authors.forename: G N
authors.initials: GN
authors.lastname: Olinger
authors.forename: T Z
authors.initials: TZ
authors.lastname: Liu
authors.forename: E R
authors.initials: ER
authors.lastname: Rodriguez
authors.forename: V J
authors.initials: VJ
authors.lastname: Ferrans
authors.forename: A H
authors.initials: AH
authors.lastname: Kissebah
mesh_terms: D000818:Animals; D001132:Arm / Q000098:blood supply; D002784:Cholesterol / Q000032:analysis*; D005263:Femoral Artery / Q000601:surgery; D005487:Foam Cells / Q000166:cytology; D007958:Leukocyte Count; D008251:Macaca; D008954:Models, Biological; D013997:Time Factors; D014680:Veins / Q000032:analysis / Q000166:cytology / Q000637:transplantation*
chemical_list: D002784:Cholesterol