[Effects of repeated skin applications of heavy pyrolysis resin on cytochrome P-450 level and glutathione transferase activity in liver microsomes and cytosol in rats in relation to the level of toxic effects of pyrolysis resin on internal organs].
Rats received 20 skin applications of heavy pyrolysis resin, containing about 30% of polycyclic aromatic hydrocarbons. The every exposure duration was 4 hours a day. Applications have been carried out daily 5 days a week in the course of 4 weeks. Induction of cytochrome P-450 (P-450) by 79%, significant induction of microsomal (GTm) and cytosol (GTc) glutathione-S-transferase activity (by 46 and 85%, respectively) and small increase of reduced glutathione level (by 9%) also were registered as a result of these exposures. Lipid peroxidation level determined by TBK-reactive products quantity didn't alter significantly. Close correlation between ratio values of P-450/GTm levels and P-450/GTc levels and toxic effect indices of heavy pyrolysis resin on rat immune and endocrine systems.
Kravchenko
M N
MN
Loginov
A S
AS
Petrova
L P
LP
Ausheva
L Kh
LKh
Bendikov
E A
EA
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis*; D003600:Cytosol / Q000737:chemistry* / Q000201:enzymology; D005982:Glutathione Transferase / Q000032:analysis*; D008297:Male; D008862:Microsomes, Liver / Q000737:chemistry* / Q000201:enzymology; D051381:Rats; D012116:Resins, Plant / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity*; D012867:Skin / Q000187:drug effects* / Q000378:metabolism; D013950:Thymus Gland / Q000187:drug effects; D013961:Thyroid Gland / Q000187:drug effects; D013997:Time Factors
D012116:Resins, Plant; D003577:Cytochrome P-450 Enzyme System; D005982:Glutathione Transferase
Vliianie povtornykh nakozhnykh applikatsiĭ tiazheloĭ smoly piroliza na uroven' tsitokhroma P-450 i aktivnost' glutationtransferazy v mikrosomakh i tsitozole pecheni u krys, vzaimosviaz' s urovnem toksicheskogo deĭstviia smoly piroliza na vnutrennie organy.