title: Temporal differences in desmin expression between myoblasts from embryonic and adult chicken skeletal muscle.
abstract: Desmin expression by myoblasts cultured from embryonic and adult chicken breast muscle was examined employing indirect immunofluorescence. The study was performed in conjunction with [3H]thymidine autoradiography and analysis of skeletal myosin expression in order to determine whether the desmin-expressing cells were terminally differentiated. Following 2 h of labeling with [3H]thymidine, 0.55%, 2.60%, and 15.10% of the cells in mass cultures from 10-day-old embryos, 18-day-old embryos and adults, respectively, incorporated [3H]thymidine and were desmin-positive but did not express skeletal-muscle-specific myosin. Using the same approach we determined that 0.07%, 1.25%, and 7.59% of the mononucleated cells in myogenic clones from 10-day-old embryos, 18-day-old embryos and adults, respectively, were desmin-positive, myosin-negative, [3H]thymidine-positive. We suggest that these desmin-positive, myosin-negative myoblasts are proliferating cells, and we conclude that the progeny of adult myoblasts exhibit more desmin-expressing cells of this type than embryonic myoblasts do.
authors.affiliation: Department of Biological Structure, School of Medicine, University of Washington, Seattle 98195.
authors.forename: Z
authors.initials: Z
authors.lastname: Yablonka-Reuveni
authors.forename: M
authors.initials: M
authors.lastname: Nameroff
mesh_terms: D000375:Aging / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens / Q000378:metabolism*; D003893:Desmin / Q000235:genetics* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D009132:Muscles / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D009218:Myosins / Q000235:genetics / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
chemical_list: D003893:Desmin; D014316:Tritium; D009218:Myosins; D013936:Thymidine