Interaction of chlorpromazine with DNA.
The mechanism of the potential anticancer agent chlorpromazine hydrochloride (CPZ) with DNA was investigated by the techniques of high-performance liquid chromatography (HPLC), viscosity and Raman spectroscopy. It has been suggested from HPLC work that DNA nucleotides (except nucleosides) from either a CPZ-DNA system or a CPZ-nucleotide system. Furthermore, the shear stress of the viscosity of the CPZ-DNA system and the CPZ-nucleotide systems ware shown to be apparently the higher increasing than that of DNA and nucleotide alone. These systems had non-Newtonian properties for the formation of the CPZ-DNA and the CPZ-nucleotide systems under experimental conditions. The Raman spectra showed a dramatic difference at 982 cm-1 due to the symmetric P-O stretching vibration of the PO4(2-) group between dGMP and the CPZ-dGMP system.
Motohashi
N
N
Meiji College of Pharmacy, Tokyo, Japan.
Kamata
K
K
Meyer
R
R
D000970:Antineoplastic Agents; D002746:Chlorpromazine / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry*; D003854:Deoxyribonucleotides / Q000737:chemistry; D009690:Nucleic Acid Conformation; D013059:Spectrum Analysis, Raman; D014783:Viscosity
D000970:Antineoplastic Agents; D003854:Deoxyribonucleotides; D004247:DNA; D002746:Chlorpromazine