Cloning and expression of the damselysin gene from Vibrio damsela.
The gene encoding damselysin, an extracellular cytolysin produced by virulent Vibrio damsela strains, was cloned and expressed in Escherichia coli. DNA sequences homologous to that of the cloned gene were detected in hemolytic strains of V. damsela but not in other hemolytic Vibrio species.
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Department of Microbiology and Immunology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, North Carolina 27103.
Kreger
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D015153:Blotting, Western; D003001:Cloning, Molecular; D003603:Cytotoxins / Q000235:genetics*; D004252:DNA Mutational Analysis; D005798:Genes, Bacterial; D006461:Hemolysis; D009693:Nucleic Acid Hybridization; D010739:Phospholipase D / Q000235:genetics*; D010740:Phospholipases / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014733:Vibrio / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity
D003603:Cytotoxins; D010740:Phospholipases; D010739:Phospholipase D