DNA flow cytometry on breast carcinomas: comparison of a detergent and an enzyme-detergent preparation method.
In this study we have compared two different preparation methods for DNA flow cytometry on breast cancer. Tumour cell suspensions from 49 breast cancers were analysed on a Facscan flow cytometer. In seven of 49 cases, additional aneuploid peaks were found after enzyme/detergent treatment (E/D), not seen after the detergent (D) preparation. S-Phase fractions were significantly higher after D than after E/D preparation (mean values, 15 and 8%, respectively), although the correlation was high between the two methods. S-Phase fraction estimated after background correction diminished the differences between the two methods (mean values, 8 and 6%). Furthermore, the fraction of G2/M cells were generally greater with the D method. These differences can be explained by increased number of cell doublets and nuclear fragments after D compared to E/D preparation. This clearly shows that the preparation method influences the result of DNA flow cytometry on human breast cancers.
Risberg
B
B
Department of Pathology, Central County Hospital, Orebro, Sweden.
Stål
O
O
Eriksson
L L
LL
Hussein
A
A
D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002453:Cell Cycle / Q000235:genetics; D002469:Cell Separation / Q000379:methods; D004273:DNA, Neoplasm / Q000032:analysis*; D003902:Detergents; D005434:Flow Cytometry; D006801:Humans; D015203:Reproducibility of Results; D012260:Ribonucleases; D014357:Trypsin
D004273:DNA, Neoplasm; D003902:Detergents; D012260:Ribonucleases; D014357:Trypsin