Developmental morphology of vascular and lymphatic capillaries in the working myocardium and Purkinje bundle of the sheep septomarginal band.
The normal development of vascular and lymphatic capillaries in the right ventricular septomarginal band of the sheep heart was studied in 9 fetuses aged 60-143 days (term = 147 days), 14 lambs aged 1 day to 16 weeks, and 3 adults. Tissue was fixed by perfusion and examined with light and transmission electron microscopy. The septomarginal band is composed of working myocardium and a well-defined peripheral bundle of Purkinje cells. Vascular capillaries of the working myocardium were closely apposed to myocardial cells. By contrast, vascular capillaries of the Purkinje bundle were situated within the connective tissue sheath and septa, at variable distances from the Purkinje cells. After birth, the capillaries of the Purkinje bundle were also found in grooves and tunnels within the Purkinje strands. The ultrastructure of fetal vascular capillaries associated with myocardial and Purkinje cells was initially similar, and characterized by an abundance of synthetic organelles in endothelial cells and pericytes. However, after 115 days in utero, capillary endothelium with diaphragmed fenestrae, 40-60 nm in width, were observed within the Purkinje bundle. The fenestrae attained an average frequency of 1 per 11 capillary cross sections just before term, and this was maintained in lambs and adults. The ultrastructure of lymphatic capillaries, which were not observed in the septomarginal band until just before term, changed little during development.
Smolich
J J
JJ
Department of Anatomy, University of Melbourne, Parkville, Australia.
Shimada
T
T
Canale
E
E
Campbell
G R
GR
D000818:Animals; D000831:Animals, Newborn / Q000033:anatomy & histology*; D005333:Fetus / Q000033:anatomy & histology*; D006321:Heart / Q000033:anatomy & histology / Q000196:embryology*; D006329:Heart Conduction System / Q000196:embryology*; D008208:Lymphatic System / Q000033:anatomy & histology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D009206:Myocardium / Q000648:ultrastructure*; D011690:Purkinje Fibers / Q000033:anatomy & histology / Q000196:embryology* / Q000648:ultrastructure; D012756:Sheep / Q000196:embryology*