Gas chromatographic mass-specific investigation of dextromoramide (Palfium) metabolism in the horse.
Dextromoramide (Palfium) was given by intravenous injection to a Thoroughbred horse at a dosage of 20 mg and urine was collected 2, 4, 6 and 8 h after drug administration. Enzymatic hydrolysis of the urine followed by solvent extraction gave a residue which was back-extracted into 0.1 M sulphuric acid. After basification to pH 9 and solvent extraction, the resulting residue was submitted to gas chromatographic-mass spectrometric analysis. Both electron-impact and ammonia chemical-ionization mass spectra were recorded and, based on the observed fragmentation patterns, the principal metabolites in horse urine were shown to be 2,2-diphenyl-3-methyl-4-morpholinobutyramide (compound 2) and the product of hydroxylation of one phenyl ring in dextromoramide (compound 3), respectively. The electron-impact mass spectra of compounds 2 and 3, and of their derivatisation products from oncolumn methylation in the gas chromatograph, are reported.
Reilly
P J
PJ
Australian Jockey Club Laboratory, Randwick, N.S.W. Australia.
Suann
C J
CJ
Duffield
A M
AM
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D003916:Dextromoramide / Q000652:urine*; D004300:Doping in Sports; D008401:Gas Chromatography-Mass Spectrometry; D006736:Horses / Q000652:urine*
D003916:Dextromoramide