Proline-specific proteases in cultivated neuronal and glial cells.
The activities of several proline-specific endo- and exopeptidases were determined in homogenates of immunocytochemically defined cultures of astrocytes, oligodendrocytes and neurones obtained from rat cerebral cortex. Astrocytes are significantly enriched in post-proline cleaving dipeptidyl peptidase II, prolidase and aminopeptidase P activities; neurones and astrocytes contain prolyl endopeptidase. Dipeptidyl peptidase IV and prolyl carboxypeptidase activities are low or absent in the cultures. The physiological significance of these findings is discussed.
Mentlein
R
R
Anatomisches Institut, Universität zu Kiel, F.R.G.
von Kolszynski
M
M
Sprang
R
R
Lucius
R
R
D000626:Aminopeptidases / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D001253:Astrocytes / Q000201:enzymology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000196:embryology / Q000201:enzymology; D009474:Neurons / Q000201:enzymology*; D009836:Oligodendroglia / Q000201:enzymology*; D000085822:Prolyl Oligopeptidases; D051381:Rats; D011919:Rats, Inbred Strains; D012697:Serine Endopeptidases / Q000378:metabolism*
D000626:Aminopeptidases; C022750:X-Pro aminopeptidase; D012697:Serine Endopeptidases; D000085822:Prolyl Oligopeptidases