Mapping regional cerebral vascular transit time by simultaneous determination of local cerebral blood flow and local cerebral blood volume.
We developed a method for autoradiographic mapping of regional cerebral transit time (CTT) by simultaneously measuring local cerebral blood flow (LCBF) and local cerebral blood volume (LCBV). Previously described single-tracer techniques for determination of LCBF and LCBV were modified for dual-tracer, 99mTc and 14C, autoradiography and used to create digital images of LCBF and LCBV from the same brain sections in a series of normal rats. The images were aligned and ratio images (LCBV/LCBF) were then generated which reflected CTT. Regional cerebral transit time was found to vary significantly through-out the brain in a pattern only partially related to that of blood flow. Such CTT heterogeneity could cause errors in implementation of kinetic models which assume uniform or monovariant distributions of vascular transit time.
Lear
J L
JL
Department of Radiology, University of Colorado Health Sciences Center, Denver 80262.
Kasliwal
R
R
Feyerabend
A
A
D000818:Animals; D001345:Autoradiography; D001783:Blood Flow Velocity; D001810:Blood Volume; D002560:Cerebrovascular Circulation; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors