Comparison of the cytotoxicity and DNA-damaging properties of 2,4-D and U 46 D fluid (dimethylammonium salt of 2,4-D).
U 46 D Fluid (the dimethylammonium salt of 2,4-dichlorophenoxyacetic acid in a commercial formulation) was more toxic to human fibroblasts than 2,4-dichlorophenoxyacetic acid (2,4-D). Moreover, U 46 D Fluid induced single-strand breaks at apurinic/apyrimidinic (AP) sites of heat-acid treated PM2 DNA while 2,4-D did not. The 1H-NMR spectrum of the dimethylammonium (DMA) salt of 2,4-D indicated the formation of a complex via a six-membered aggregate of the DMA ion and the acid group of 2,4-D. The role of complex formation of the DMA salt of 2,4-D as a cause of single-strand breaking activity and increased cytotoxicity is discussed.
Clausen
M
M
Universität Oldenburg, Fachbereich Biologie, Federal Republic of Germany.
Leier
G
G
Witte
I
I
D015084:2,4-Dichlorophenoxyacetic Acid / Q000633:toxicity*; D000143:Acids; D002470:Cell Survival / Q000187:drug effects*; D003114:Colony-Forming Units Assay; D004249:DNA Damage; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006358:Hot Temperature; D006801:Humans; D009682:Magnetic Resonance Spectroscopy
D000143:Acids; D015084:2,4-Dichlorophenoxyacetic Acid