[Spectrum pattern and determination of ganglioside from human primary liver cancer of different origins].
Ganglioside on the cell membrane surface was isolated from human primary liver cancer of different origins (10 surgical specimens from patients, 4 tumor tissues from nude rats bearing transplanted human liver cancer and 2 human liver cancer cell lines) and compared with that of normal liver from men and nude rats. The results of TLC pattern showed that GM3 was the main constituent, comprising about 95% of the total gangliosides in the normal liver. However, it decreased in liver cancer whereas GM2 was increased markedly in most of the liver cancers, GD3 came next. By CS-910 scanning, the contents of GM3, GM2 and GD3 were 60.2%, 20.6% and 7.9% in surgical specimens; 46.1%, 29.7% and 11.9% in nude rats bearing human liver cancer and 25.2%, 47.6% and 0% in cell lines. GM2 and GD3 on the cell surface of liver cancer were determined with monoclonal antibodies or anti-serum against gangliosides. The results obtained conformed with the TLC pattern.
Lin
Z Y
ZY
Institute of Liver Cancer, Shanghai Medical College.
D000818:Animals; D000911:Antibodies, Monoclonal; D002855:Chromatography, Thin Layer; D005678:G(M2) Ganglioside / Q000032:analysis / Q000276:immunology; D005679:G(M3) Ganglioside / Q000032:analysis; D005732:Gangliosides / Q000032:analysis* / Q000276:immunology; D006801:Humans; D008113:Liver Neoplasms / Q000737:chemistry* / Q000276:immunology / Q000473:pathology; D009368:Neoplasm Transplantation; D051381:Rats; D011923:Rats, Nude; D014407:Tumor Cells, Cultured / Q000737:chemistry
D000911:Antibodies, Monoclonal; D005679:G(M3) Ganglioside; D005732:Gangliosides; D005678:G(M2) Ganglioside; C026226:ganglioside, GD3