Isolation of cytotoxic substance, kalafungin from an alkalophilic actinomycete, Nocardiopsis dassonvillei subsp. prasina.
An alkalophilic actinomycete, strain OPC-553 regarded as Nocardiopsis dassonvillei subsp. prasina, produced the cytotoxic substance, TS-1, which showed a marked inhibitory activity against L5178Y mouse leukemic cell in vitro. The cytotoxicity of TS-1 on this cell was very strong and its ID50 was 0.018 micrograms/ml. Through direct comparison of its spectral data with those of an authentic sample, TS-1 was identified as the antifungal antibiotic, kalafungin, already isolated from the culture broth of Streptomyces tanashiensis. However, the isolation of kalafungin from an alkalophilic actinomycete and its cytotoxicity are reported for the first time in this paper.
Tsujibo
H
H
Osaka University of Pharmaceutical Sciences, Japan.
Sakamoto
T
T
Miyamoto
K
K
Kusano
G
G
Ogura
M
M
Hasegawa
T
T
Inamori
Y
Y
D000192:Actinomycetales / Q000032:analysis*; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000302:isolation & purification* / Q000494:pharmacology; D000935:Antifungal Agents / Q000302:isolation & purification* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D007940:Leukemia L5178 / Q000188:drug therapy*; D051379:Mice; D009285:Naphthoquinones / Q000302:isolation & purification / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects; D000095622:Benzoisochromanequinones
D000903:Antibiotics, Antineoplastic; D000935:Antifungal Agents; D009285:Naphthoquinones; C013079:kalafungin; D000095622:Benzoisochromanequinones