Interaction of extracellular vesicles of Bacteroides gingivalis W50 with human polymorphonuclear leucocytes.
The effects of B. gingivalis W50 extracellular vesicles (ECV) on neutrophil chemotaxis and viability were assessed and compared with those of whole cells and the extracellular non-dialysable soluble protein (EP) fraction. None of the fractions tested, including soluble fractions derived from cells and ECV by sonication, induced neutrophil chemotaxis. Only ECV and cells inhibited f-MLP-stimulated chemotaxis. ECV and cells were cytotoxic towards neutrophils. The cytotoxic response was time dependent. The soluble EP fraction did not influence cell viability.
Kay
H M
HM
Department of Clinical Dental Sciences, University of Liverpool, U.K.
Birss
A J
AJ
Smalley
J W
JW
D001439:Bacteroides / Q000472:pathogenicity* / Q000648:ultrastructure; D002470:Cell Survival; D002634:Chemotaxis, Leukocyte; D006801:Humans; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009504:Neutrophils / Q000166:cytology / Q000502:physiology*; D012995:Solubility; D014774:Virulence
D009240:N-Formylmethionine Leucyl-Phenylalanine