Active suppression of the proliferative response to interleukin-2 in tumour-bearing mice.
Spleen cells from mice bearing progressively growing syngeneic methylcholanthrene-induced sarcomas are immunologically hyporeactive and also their proliferative responses to interleukin-1 (IL-1) or interleukin-2 (IL-2) stimulation are considerably decreased. The hypo-reactivity to IL-2, but not to IL-1, is due to an active suppression: spleen cells from tumour-bearing mice inhibit the IL-2-induced proliferation of cells from normal donors. Supernatants obtained after cultivation of spleen cells from mice bearing tumours show similar suppressive effects. The hyporeactivity to IL-2 and IL-1 in tumour-bearerers is not improved by indomethacin, an inhibitor of prostaglandin synthesis. The results show that the low reactivity to IL-2 in tumour-bearing mice is due to an active suppression mediated by spleen cells and their factor(s) and that more different mechanisms regulate responsiveness to interleukins in tumour-bearing hosts.
Zajícová
A
A
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
Holán
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V
D000818:Animals; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003470:Culture Media; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000276:immunology* / Q000494:pharmacology; D007376:Interleukin-2 / Q000276:immunology* / Q000494:pharmacology; D008297:Male; D008748:Methylcholanthrene; D051379:Mice; D008810:Mice, Inbred C57BL; D011994:Recombinant Proteins / Q000276:immunology / Q000494:pharmacology; D012513:Sarcoma, Experimental / Q000139:chemically induced / Q000276:immunology*; D013154:Spleen / Q000187:drug effects / Q000276:immunology*; D013491:Suppressor Factors, Immunologic / Q000096:biosynthesis* / Q000502:physiology
D003470:Culture Media; D007375:Interleukin-1; D007376:Interleukin-2; D011994:Recombinant Proteins; D013491:Suppressor Factors, Immunologic; D008748:Methylcholanthrene; D007213:Indomethacin