A selective defect in tissue factor mRNA expression in monocytes from AIDS patients.
Expression of tumor necrosis factor (TNF alpha), tissue factor (TF), and interleukin 1-beta (IL-1 beta) mRNA was evaluated in monocytes isolated from patients infected with human immunodeficiency virus (HIV). There was a significant depression (66%) of the induced level of TF mRNA expression in response to lipopolysaccharide. Conversely, the response of TNF alpha and IL-1 beta, following LPS induction, was "normal." TF mRNA reduction was also observed to a lesser degree in AIDS-related complex patients (20%) but not in asymptomatic seropositives. TF is necessary for initiation of the coagulation protease cascade, leading to thrombin production and fibrin deposition, which play a role in inflammatory responses. Its selective reduction may be a factor in the diminished resistance to secondary infections observed in AIDS. Further, since the TF defect increases as patients progress toward AIDS, it may serve as a marker for disease progression.
Lathey
J L
JL
Department of Immunology, Research Institute of Scripps Clinic, La Jolla, California 92037.
Agosti
J M
JM
Nelson
J A
JA
Corey
L
L
Gregory
S A
SA
Morrissey
J H
JH
Edgington
T S
TS
Oldstone
M B
MB
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood* / Q000276:immunology; D000328:Adult; D001777:Blood Coagulation; D006801:Humans; D006968:Hypersensitivity, Delayed; D007375:Interleukin-1 / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D009000:Monocytes / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis*; D013925:Thromboplastin / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D007375:Interleukin-1; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D013925:Thromboplastin