Metastatic potential of colon carcinoma. Expression of ABO/Lewis-related antigens.
Four monoclonal antibodies specific to various Lewisx-related antigens were tested for binding to monolayers and detergent extracts of the human colon carcinoma cell line HT-29 P and its metastatic variant HT-29 LMM. Only monoclonal antibody FH6 (antisialyl-dimeric Lex) bound more to the metastatic variant compared with HT-29 P. Detergent extracts of human tissues were then assayed for their FH6-binding activity. Normal colonic mucosa displayed less FH6 binding than either Dukes stage B or D primary colon carcinomas. Liver metastases from colorectal carcinoma demonstrated greater binding than stage B and stage D primary tumors, and more than normal liver. Expression of sialyl-dimeric Lex increases as the metastatic potential of colorectal carcinomas increases, and is related to the progression of colorectal cancer.
Hoff
S D
SD
Department of General Surgery, University of Texas M. D. Anderson Cancer Center, Houston 77030.
Irimura
T
T
Matsushita
Y
Y
Ota
D M
DM
Cleary
K R
KR
Hakomori
S
S
D000017:ABO Blood-Group System; D000230:Adenocarcinoma / Q000276:immunology* / Q000556:secondary; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis*; D002241:Carbohydrates / Q000276:immunology; D002277:Carcinoma / Q000276:immunology / Q000556:secondary; D003106:Colon / Q000276:immunology; D003110:Colonic Neoplasms / Q000276:immunology*; D006801:Humans; D007413:Intestinal Mucosa / Q000276:immunology; D007519:Isoantigens / Q000032:analysis*; D007983:Lewis Blood Group Antigens; D008099:Liver / Q000276:immunology; D008113:Liver Neoplasms / Q000276:immunology / Q000556:secondary; D014407:Tumor Cells, Cultured
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D002241:Carbohydrates; D007519:Isoantigens; D007983:Lewis Blood Group Antigens