Conversion of 11-ketoandrostenedione to 11-ketotestosterone by blood cells of six fish species.
Blood cells from Baltic salmon, Salmo salar, three-spined stickleback, Gasterosteus aculeatus, eel pout, Zoarces viviparus, crucian carp, Carassius carassius, African catfish, Clarias gariepinus, and reedfish, Calamoichthys calabaricus, were incubated with tritiated 11 beta-hydroxyandrostenedione (OHA) or 11-ketoandrostenedione (OA). In all fish there was conversion of OA to 11-ketotestosterone (OT), indicating that 17 beta-hydroxysteroid dehydrogenase activity was present in the blood cells. On the other hand, OHA was not converted to 11 beta-hydroxytestosterone in any fish. The addition of serum to the incubates largely prevented the OA-OT conversion by salmon blood cells.
Mayer
I
I
Department of Zoology, University of Stockholm, Sweden.
Borg
B
B
Schulz
R
R
D000736:Androstenes / Q000097:blood*; D000818:Animals; D001711:Biotransformation; D002347:Carps / Q000097:blood; D002397:Catfishes / Q000097:blood; D004524:Eels / Q000097:blood; D005399:Fishes / Q000097:blood*; D066298:In Vitro Techniques; D012474:Salmon / Q000097:blood; D013045:Species Specificity; D013739:Testosterone / Q000031:analogs & derivatives* / Q000097:blood
D000736:Androstenes; D013739:Testosterone; C011657:adrenosterone; C003600:11-ketotestosterone