Relaxations of guinea-pig isolated trachea induced by platelet-activating factor are epithelial-dependent and are antagonised by WEB 2086.
Platelet-activating factor (PAF, 10-1000 pmol) induced dose-dependent relaxations of the basal tone of superfused strips of epithelium-intact guinea-pig trachea. Indomethacin (1.4 microM) completely inhibited and WEB 2086 (1 and 10 nM) effectively antagonised these relaxations. Following epithelial removal PAF evoked a single contraction. These results show that the PAF-induced relaxations of guinea-pig trachea are dependent on an intact epithelial layer and are mediated by a cyclo-oxygenase product.
Conroy
D M
DM
Department of Pharmacology, Hunterian Institute, Royal College of Surgeons of England, U.K.
Samhoun
M N
MN
Piper
P J
PJ
D000420:Albuterol / Q000494:pharmacology; D000818:Animals; D001381:Azepines / Q000494:pharmacology*; D004848:Epithelium / Q000502:physiology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects*; D010972:Platelet Activating Factor / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D014132:Trachea / Q000187:drug effects; D014230:Triazoles / Q000494:pharmacology*
D001381:Azepines; C029271:O-deacetyl platelet activating factor; D010972:Platelet Activating Factor; D014230:Triazoles; C052518:WEB 2086; D000420:Albuterol; D007213:Indomethacin