[Use of various cell culture antigens in the indirect fluorescent antibody test for the detection of antinuclear antibodies].
Cell culture antigens were prepared from Vero, BHK-21 and Hep-2 cells which were propagated on slide, for detection antinuclear antibody (ANA) in sera with indirect fluorescent antibody test (IFAT). ANA were investigated in 55 sera which were positive at 1/20 titer with KB IFAT ANA test kits (Virgo), by using these cell culture antigens. 50 sera (91%) with Vero antigens, 46 sera (83%) with BHK-21 antigens and 44 sera (80%) with Hep-2 antigens were found positive at 1/20 titer. As conclusion Vero cell culture which is propagated on slide can be used as antigen for detection of ANA with IFAT.
Kocabeyoğlu
O
O
Ulusal Klinik Mikrobiyoloji ve Infeksiyon Hastaliklari Kongresi'nde, Mikrobiyoloji Ana Bilim Dali Oğretim Uyesi.
Emekdaş
G
G
Yücel
N
N
Ozcan
N
N
D000818:Animals; D000974:Antibodies, Antinuclear / Q000097:blood*; D002460:Cell Line / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D011933:Reagent Kits, Diagnostic; D014709:Vero Cells / Q000276:immunology
D000974:Antibodies, Antinuclear; D011933:Reagent Kits, Diagnostic
Indirekt fluoresan antikor test ile antinükleer antikor saptanmasinda çeşitli hücre kültürü antijenlerinin kullanilmasi.