[Identification and isolation of the protein insect toxin (alpha-latroinsectotoxin from venom of the spider Latrodectus mactans tredecimguttatus].
The crude venom of spider Latrodectus mactans tredecimguttatus was fractionated by the combination of anion exchange and hydrophobic chromatography. The biological activity of fraction was tested by means of: 1) estimation of toxicity for housefly larva; 2) intracellular recording of miniature excitatory potentials (MEPSPs) in blowfly larvae muscle fibres. As a result of sequential procedures of chromatography separation a homogeneous protein of 120 kilodalton molecular weight was obtained. This protein referred to alpha-latroinsectotoxin produced: 1) a great increase of the frequency of MEPSPs in the dose of 4.2.10(-10) M and its paralytic dose for fly larva was approximately 20 ng/species; 2) no influence of the MEPSPs after application in the dose of 1.2.10(-7) M to the neuromuscular junction of the frog.
Kovalevskaia
G I
GI
Pashkov
V N
VN
Bulgakov
O V
OV
Fedorova
I M
IM
Magazanik
L G
LG
Grishin
E V
EV
D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D001001:Anura; D001740:Black Widow Spider; D002852:Chromatography, Ion Exchange; D004175:Diptera; D004591:Electrophoresis, Polyacrylamide Gel; D005071:Evoked Potentials / Q000187:drug effects; D066298:In Vitro Techniques; D013111:Spider Venoms / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity
D000596:Amino Acids; D013111:Spider Venoms; C020763:alpha-latrotoxin
Identifikatsiia i vydelenie belkovogo insektotoksina (alpha- latroinsektotoksina) iz iada pauka Latrodectus mactans tredecimguttatus.