Detection of hippurate hydrolysis by Legionella spp. by using a rapid high-performance liquid chromatography method.
A rapid high-performance liquid chromatography method was developed to determine hippurate hydrolysis by Legionella spp. Benzoic acid, an end product of enzymatic activity, was directly detected by high-performance liquid chromatography after 1 and 24 h of incubation in 1% sodium hippurate. Because of its sensitivity, this procedure offers more precise identification of some Legionella spp.
Marmet
D
D
Laboratoire National de la Santé, Centre National de Référence des Légionelloses, Faculté de Médecine, Lyon, France.
Bornstein
N
N
Fleurette
J
J
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006626:Hippurates / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D007875:Legionella / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D013045:Species Specificity
D006626:Hippurates; C030514:hippuric acid