Concurrent on-line sampling of melatonin in pineal microdialysates from conscious rat and its analysis by high-performance liquid chromatography with electrochemical detection.
Dynamic changes of melatonin in microdialysates from the pineal gland of a freely moving rat were repeatedly determined by using on-line high-performance liquid chromatography with electrochemical detection. The detection limit for melatonin, ca. 5 pg, was well below that achieved with other systems. We observed a drastic increase of extracellular pineal melatonin during the transitional phase from the light period to the dark period. This application of microdialysis is a useful tool in the study of the physiological role of the mammalian pineal body.
Azekawa
T
T
Department of Physiology, School of Medicine, University of Tokushima, Japan.
Sano
A
A
Aoi
K
K
Sei
H
H
Morita
Y
Y
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D002940:Circadian Rhythm; D003956:Dialysis; D008297:Male; D008550:Melatonin / Q000032:analysis* / Q000378:metabolism; D008832:Microchemistry; D010870:Pineal Gland / Q000737:chemistry* / Q000378:metabolism; D011786:Quality Control; D051381:Rats; D011919:Rats, Inbred Strains
D008550:Melatonin