Nucleic acid sequence of cDNA (RSD-1) encoding a rabbit sperm membrane protein and structural properties of the encoded protein.
The nucleotide sequence of a 2.0 kb cDNA encoding a rabbit sperm tail membrane protein was determined using the chain termination method. Analysis was performed using Sequenase and Klenow enzymes on the target sequence in an M13mp18/19 cloning system. The open reading frame consisted of 438 bp encoding 146 amino acids of the protein, with a calculated Mr of 15507. The hydrophobic amino acid content was 34.9%, and pI was in the vicinity of 5.8.
Yun
B
B
Institute of Basic Medical Sciences, CAMS, Beijing.
Miao
S
S
Yan
Y
Y
Wang
L
L
Koide
S S
SS
D000595:Amino Acid Sequence; D000818:Animals; D000954:Antigens, Surface; D001483:Base Sequence; D003062:Codon; D004247:DNA / Q000235:genetics*; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011817:Rabbits
D000954:Antigens, Surface; D003062:Codon; D008565:Membrane Proteins; C049242:rSMP-B protein, human; D004247:DNA