title: Testing the notion of the one-hit exchange.
abstract: Classical theory asserts that radiation-induced chromosomal exchanges result from the interaction of lesions on both chromosomes involved, a notion supported by substantial indirect evidence, but more recently questioned on biophysical and molecular grounds. When mitotic HeLa cells were irradiated with 60Co gamma rays, and fused together with Sendai virus, numerous chromosome exchanges were observed between the genomes of different cells at the next mitosis. However, when irradiated and unirradiated cells were fused together, the frequency of intergenomic exchange was 40-fold lower, suggesting that the vast majority of radiation-induced exchanges do, in fact, require damage to both chromosomes.
authors.affiliation: Life Sciences Division, Los Alamos National Laboratory, New Mexico 87545.
authors.forename: M N
authors.initials: MN
authors.lastname: Cornforth
mesh_terms: D002459:Cell Fusion; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000528:radiation effects*; D003037:Cobalt Radioisotopes; D005720:Gamma Rays; D015894:Genome, Human; D015726:Giant Cells; D006367:HeLa Cells / Q000528:radiation effects; D006801:Humans; D008938:Mitosis
chemical_list: D003037:Cobalt Radioisotopes