title: Fresh versus cryopreserved cultured allografts for the treatment of chronic skin ulcers.
abstract: Both fresh and cryopreserved cultured epithelial allografts were used to treat 20 patients with a total of 30 chronic ulcers. A profound stimulation of host epithelialization from the wound edges and epidermal appendages was observed. Our data suggest that allografts, prepared using a simple cryopreservation technique, retain their morphological and functional characteristics, and are as effective as fresh allografts in healing chronic ulceration.
authors.affiliation: Department of Dermatology, University Hospital Leiden, The Netherlands.
authors.forename: R G
authors.initials: RG
authors.lastname: Teepe
authors.forename: E J
authors.initials: EJ
authors.lastname: Koebrugge
authors.forename: M
authors.initials: M
authors.lastname: Ponec
authors.forename: B J
authors.initials: BJ
authors.lastname: Vermeer
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002908:Chronic Disease; D015925:Cryopreservation; D046508:Culture Techniques; D005260:Female; D006801:Humans; D015603:Keratinocytes / Q000637:transplantation*; D008297:Male; D008875:Middle Aged; D012883:Skin Ulcer / Q000601:surgery*; D013997:Time Factors; D014184:Transplantation, Homologous; D014945:Wound Healing / Q000502:physiology