Synovial fluid does not inhibit collagen synthesis. Bovine cruciate ligament studied in vitro.
An organ culture model of four bovine anterior cruciate ligaments was used to compare synovial fluid with bovine serum, lactated Ringer's solution, and Basal Medium Eagle's (BME) to determine their relative abilities to support in vitro conversion of proline into hydroxyproline. There was no difference between serum and synovial fluid in their ability to support collagen synthesis. These results suggest that bovine synovial fluid is not inhibitory in that it is similar to bovine serum in supporting ligament collagen synthesis under the conditions described.
Salo
P
P
Department of Surgery, Faculty of Medicine, University of Calgary, Alberta, Canada.
Frank
C
C
Marchuk
L
L
D000818:Animals; D016118:Anterior Cruciate Ligament / Q000254:growth & development*; D002417:Cattle; D003094:Collagen / Q000096:biosynthesis*; D003470:Culture Media; D006909:Hydroxyproline / Q000378:metabolism; D007552:Isotonic Solutions; D009924:Organ Culture Techniques; D011392:Proline / Q000378:metabolism; D000077325:Ringer's Lactate; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013582:Synovial Fluid
D003470:Culture Media; D007552:Isotonic Solutions; D000077325:Ringer's Lactate; D012710:Serum Albumin, Bovine; D003094:Collagen; D011392:Proline; D006909:Hydroxyproline