Induction of peroxisomal enzymes in cultured porcine endothelial cells by the hypolipidemic drug ciprofibrate.
The purpose of this study was to determine if the hypolipidemic peroxisome proliferator ciprofibrate, which induces peroxisomes in the liver, can induce peroxisomes in cultured porcine pulmonary endothelial cells. Ciprofibrate was added at three concentrations to cell cultures for a 6-day period. The induction of peroxisomes in the cells was detected by determining total peroxisomal beta-oxidation and peroxisomal catalase activity. The addition of ciprofibrate was found to increase peroxisomal enzyme activities in a dose-dependent manner, with the highest activity being reached at 1000 microM ciprofibrate. Ciprofibrate also caused an increased transfer of albumin across endothelial cells cultured on micropore filters. This study shows that peroxisomal enzyme activities can be induced by ciprofibrate in endothelial cells, which may have implications in diseases mediated by vascular injury.
Glauert
H P
HP
Department of Nutrition and Food Science, University of Kentucky, Lexington 40506.
Hennig
B
B
Chow
H S
HS
D000818:Animals; D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D002995:Clofibric Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D004727:Endothelium / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D004790:Enzyme Induction; D058607:Fibric Acids; D000960:Hypolipidemic Agents / Q000494:pharmacology*; D008168:Lung / Q000166:cytology / Q000378:metabolism; D008830:Microbodies / Q000201:enzymology*; D010084:Oxidation-Reduction; D013552:Swine
D058607:Fibric Acids; D000960:Hypolipidemic Agents; D002995:Clofibric Acid; D002374:Catalase; C019304:ciprofibrate