A new plasmidic cefotaximase in a clinical isolate of Escherichia coli.
Escherichia coli GRI was isolated from an ear exudate of a newborn. The strain was highly resistant to cefotaxime (MIC 128 mg/l). Resistance to cefotaxime and the majority of beta-lactam antibiotics was readily transferred to an Escherichia coli recipient strain. Both the wild type and the transconjugant strains are different in their resistance phenotype from TEM-3 beta-cefotaximase producers by higher MICs to the majority of beta-lactams and lower MICs to ceftazidime. The isoelectric point of the cefotaximase of E. coli GRI was 8.9 in comparison with 6.3 for TEM-3. Thus, the enzyme produced by E. coli GRI represents a new plasmidic (plasmid pMVP-3) broad spectrum beta-lactamase (CTX-M) which may not be closely related to either the TEM- oder SHV-family of extended broad spectrum beta-lactamases.
Bauernfeind
A
A
Max-von-Pettenkofer-Institut, München, Germany.
Grimm
H
H
Schweighart
S
S
D002439:Cefotaxime / Q000378:metabolism; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000032:analysis; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D004927:Escherichia coli Infections / Q000382:microbiology*; D006801:Humans; D007223:Infant; D007526:Isoelectric Point; D010034:Otitis Media with Effusion / Q000382:microbiology*; D011815:R Factors; D001618:beta-Lactamases / Q000737:chemistry / Q000235:genetics*
D004269:DNA, Bacterial; D001618:beta-Lactamases; D002439:Cefotaxime