Oxygen radicals mediate depletion of pancreatic sulfhydryl compounds in rats with cerulein-induced acute pancreatitis.
Acute edematous pancreatitis was induced in conscious rats by intravenous infusion of cerulein at a supramaximal dose of 7.5 micrograms/kg/h during 6 h. The most important finding of our study was a marked decrease in the protein and non-protein content of sulfhydryl groups parallel to an evident elevation in the malondialdehyde concentration in pancreatic tissue. The presented data suggest that in cerulein-induced acute pancreatitis in rats, oxygen radicals mediate increased peroxidation reactions which are accompanied by depletion of nonenzymatic sulfhydryl-containing free radical scavengers. The above phenomenon contributes to a disturbance in thiol metabolism resulting in serious diminution of pancreatic protein sulfhydryl compounds.
Dabrowski
A
A
Department of Gastroenterology, Medical Academy, Białystok, Poland.
Chwiećko
M
M
D000208:Acute Disease; D000818:Animals; D002108:Ceruletide; D005609:Free Radicals; D008297:Male; D010100:Oxygen / Q000378:metabolism*; D010179:Pancreas / Q000378:metabolism*; D010195:Pancreatitis / Q000139:chemically induced* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013438:Sulfhydryl Compounds / Q000378:metabolism*
D005609:Free Radicals; D013438:Sulfhydryl Compounds; D002108:Ceruletide; D010100:Oxygen