Prenylated proteins: the structure of the isoprenoid group.
The mevalonate-derived portion of a prenylated protein from Chinese hamster ovary cells has been established as diterpenoid (C20). This group is linked to a carboxyl-terminal cysteine as a thioether. It was removed from the protein by hydrazinolysis followed by Raney nickel desulfurization, and the resulting hydrocarbon fraction was analyzed by gas chromatography-mass spectrometry.
Rilling
H C
HC
Department of Biochemistry, University of Utah, Salt Lake City 84112.
Breunger
E
E
Epstein
W W
WW
Crain
P F
PF
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004224:Diterpenes / Q000378:metabolism*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D008798:Mevalonic Acid / Q000378:metabolism; D015394:Molecular Structure; D010053:Ovary; D011498:Protein Precursors / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000378:metabolism*
D004224:Diterpenes; D011498:Protein Precursors; D011506:Proteins; D008798:Mevalonic Acid