Correlation of migration behavior in free-flow zone electrophoresis and electrophoretic titration curve.
The correlation of electrophoretic migration behavior in free-flow zone electrophoresis (FFZE) and electrophoretic titration curve (ETC) has been explored. It is shown that the ETC of a protein or a mixture of proteins can be used to predict the fraction numbers at which those proteins elute in a preparative scale FFZE experiment. The ETC is a quick and effective way to choose optimal buffer conditions in FFZE. FFZE is employed to determine the isoelectric points (pI) of proteins whose pIs lie beyond the range of IEF 3-9 gels. It is found that separations in FFZE are governed by the net surface charge of the proteins.
Nath
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Gesellschaft für Biotechnologische Forschung, Braunschweig, Federal Republic of Germany.
Schütte
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H
Hustedt
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H
Deckwer
W D
WD
D002021:Buffers; D004586:Electrophoresis; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D011506:Proteins / Q000737:chemistry
D002021:Buffers; D011506:Proteins