title: Prognostic value of flow cytometric DNA content analysis in granulosa cell tumor of the ovary.
abstract: The nuclear DNA content and S-phase fraction of 23 ovarian granulosa cell tumors were measured from paraffin-embedded tissue with flow cytometry. Crude survival of the patients with a euploid tumor (17 diploid, one tetraploid) was more favorable than that of the patients with an aneuploid tumor (n = 5, P = 0.02). The percentage of S-phase cells was a good predictor of survival. If more than 6% S-phase cells were present in the DNA histogram, both crude survival (P = 0.0001) and survival corrected for intercurrent deaths (P = 0.0001) were clearly inferior as compared with tumors with less than 6% S-phase cells. The results indicate that DNA flow cytometric study may provide a rapid and valuable method to predict the biological behavior of granulosa cell tumors of the ovary.
authors.affiliation: Department of Pathology, University Central Hospital, Turku, Finland.
authors.forename: P J
authors.initials: PJ
authors.lastname: Klemi
authors.forename: H
authors.initials: H
authors.lastname: Joensuu
authors.forename: T
authors.initials: T
authors.lastname: Salmi
mesh_terms: D000328:Adult; D000368:Aged; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D006106:Granulosa Cell Tumor / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D006801:Humans; D008875:Middle Aged; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D011003:Ploidies; D011379:Prognosis; D012008:Recurrence; D016019:Survival Analysis
chemical_list: D004273:DNA, Neoplasm