Targeting of plant glycoside-bearing liposomes to specific cellular and subcellular sites.
The possibility of using liposomes as an effective drug delivery system has been studied by incorporation of two plant glycosides of varying terminal sugar residues onto the surface of liposomes and examination of their distribution in different tissues. The two glycosides, corchorusin D and asiaticoside having glucose and rhamnose respectively at the terminal ends wee selected for the purpose. The hepatic uptake of liposomes made from egg lecithin, cholesterol and dicetyl phosphate and either of the two glycosides was compared. The hepatic uptake of asiaticoside bearing liposomes was reduced, whereas that of corchorusin D bearing liposomes was enhanced and was specific for glucose. Liver perfusion followed by cell separation showed that the uptake is mostly into the non-parenchymal cells of liver. The distribution of corchorusin D bearing liposomes was maximal in the lysosomal fraction of the non-parenchymal cells. Ways of using corchorusin D bearing liposomes as delivery systems for drugs or enzymes to lysosomes have been sought.
Medda
S
S
Biomembrane Division, Indian Institute of Chemical Biology, Calcutta.
Das
N
N
Bachhawat
B K
BK
Mahato
S B
SB
Basu
M K
MK
D000372:Agglutination Tests; D000818:Animals; D003208:Concanavalin A; D004337:Drug Carriers; D005260:Female; D005949:Glucose Oxidase / Q000008:administration & dosage; D006027:Glycosides / Q000008:administration & dosage*; D007917:Leprostatic Agents / Q000008:administration & dosage*; D008081:Liposomes; D008099:Liver / Q000166:cytology / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism; D008297:Male; D015394:Molecular Structure; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism*; D014315:Triterpenes / Q000008:administration & dosage*
D004337:Drug Carriers; D006027:Glycosides; D007917:Leprostatic Agents; D008081:Liposomes; D014315:Triterpenes; C067483:corchorusin D; D003208:Concanavalin A; D005949:Glucose Oxidase; C004446:asiaticoside