Biochemical properties of a P2Y-purinergic receptor.
The turkey erythrocyte has substantial value as a model for the study of a receptor that exhibits pharmacological properties very similar to those delineated in mammalian tissues for a P2Y-purinergic receptor. The G protein-dependent coupling of this receptor to phospholipase C can be studied in detail, and the availability of an abundant source of homogeneous cells from which highly purified plasma membranes can be prepared, has led to the development of a radiolabeled, reversibly binding radioligand for a P2Y-purinergic receptor and a photoaffinity covalent radiolabel for this receptor. This source of plasma membranes highly enriched in P2Y-purinergic receptors should also serve as a rich starting material for the eventual purification and structural characterization of this important signaling protein.
Harden
T K
TK
Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill 27599.
Boyer
J L
JL
Brown
H A
HA
Cooper
C L
CL
Jeffs
R A
RA
Martin
M W
MW
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000818:Animals; D004912:Erythrocytes / Q000378:metabolism*; D007700:Kinetics; D011983:Receptors, Purinergic / Q000187:drug effects / Q000378:metabolism*; D014422:Turkeys
D011983:Receptors, Purinergic; D000255:Adenosine Triphosphate