PKC cellular distribution in TPA activated human peripheral blood mononuclear cells, treated with an anti-HLA class I monoclonal antibody.
Cytosolic and Particulate Protein Kinase C has been studied in Peripheral Blood Mononuclear Cells activated with 12-O-Tetradecanoyl phorbol 13-acetate and treated with the anti-HLA Class I Monoclonal Antibody 01.65. No effects on the cellular distribution of PKC activity nor to the proliferative response has been found. In phytohemagglutinin stimulated PBMC cultures treated with MoAb 01.65 total PKC activity depletion and 3H-Thymidine incorporation inhibition has been found. In PBMC cultures activated with both PHA and TPA, the proliferative response was similar to cultures activated with PHA alone, while the PKC cellular distribution was similar to the one detected in TPA stimulated cultures. Addition of the MoAb 01.65 was ineffective on both PKC activity and 3H-Thymidine incorporation. These data indicate that anti-HLA Class I MoAb induced 3H-Thymidine incorporation inhibition may be related to low levels of PKC activity.
Orlando
P
P
Istituto di Genetica Medica, Università degli Studi di Ferrara, Italy.
Selvatici
R
R
Rubini
M
M
Balboni
A
A
Gandini
E
E
D000911:Antibodies, Monoclonal / Q000276:immunology; D002478:Cells, Cultured; D003600:Cytosol / Q000201:enzymology; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000201:enzymology*; D011493:Protein Kinase C / Q000097:blood*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D013936:Thymidine