title: Mechanism of action of the nitrosoureas--IV. Synthesis of the 2-haloethylnitrosourea-induced DNA cross-link 1-(3-cytosinyl),2-(1-guanyl)ethane.
abstract: The 2-haloethylnitrosoureas have been shown to form the cross-linked structure 1-(3-cytosinyl),2-(1-guanyl)ethane in DNA. This cross-link has now been synthesized by the reaction of O6-(2-fluoroethyl)guanosine with deoxycytidine in dimethyl sulfoxide followed by removal of the sugars by acid hydrolysis. This synthetic route supports the mechanism for cross-link formation in DNA that involves an initial attack on the O6-position of guanine, followed by a rearrangement and subsequent reaction with cytosine. It also provides a practical route to the synthesis of 1-(3-cytosinyl),2-(1-guanyl)ethane for studies involving formation of this cross-link in DNA.
authors.affiliation: Department of Pharmacology, University of Massachusetts Medical School, Worcester 01655.
authors.forename: J G
authors.initials: JG
authors.lastname: MacFarland
authors.forename: M C
authors.initials: MC
authors.lastname: Kirk
authors.forename: D B
authors.initials: DB
authors.lastname: Ludlum
mesh_terms: D000970:Antineoplastic Agents; D055598:Chemical Phenomena; D002621:Chemistry; D003432:Cross-Linking Reagents; D004247:DNA / Q000187:drug effects*; D003841:Deoxycytidine; D015226:Dinucleoside Phosphates / Q000138:chemical synthesis*; D006151:Guanosine / Q000031:analogs & derivatives; D013058:Mass Spectrometry; D009607:Nitrosourea Compounds / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000970:Antineoplastic Agents; D003432:Cross-Linking Reagents; D015226:Dinucleoside Phosphates; D009607:Nitrosourea Compounds; D003841:Deoxycytidine; D006151:Guanosine; C062310:1-(3-cytosinyl)-2-(1-guanyl)ethane; C018296:fluoroethylguanosine; D004247:DNA