title: Changes in catalase activity in lung and liver after endotoxemia in sheep.
abstract: Endotoxemia in the sheep produces oxidant-induced cardiopulmonary dysfunction and lung and liver lipid peroxidation, which can be prevented with exogenous catalase, indicating a role for hydrogen peroxide. We determined whether endotoxin-induced oxidant release altered endogenous catalase activity to help explain the lipid peroxidation. Unanesthetized sheep were given 2 micrograms/kg Escherichia coli endotoxin and killed at 5 hr or 24 hr. Lung and liver lipid peroxidation, measured as malondialdehyde, and catalase activity were determined after endotoxin and compared with controls. Lung tissue MDA increased by 100% at 5 hr and was still elevated by 50% at 24 hr, while catalase activity decreased by 50% at 5 hr and remained decreased, suggesting irreversible inactivation. Liver MDA was also doubled at both 5 and 24 hr, but catalase activity remained unchanged. We conclude that endotoxemia results in a significant inactivation of endogenous catalase activity in lung, but not in liver. The lung may be more prone to a subsequent H2O2 injury before restoration of catalase activity.
authors.affiliation: Longwood Area Trauma Center, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115.
authors.forename: R
authors.initials: R
authors.lastname: Daryani
authors.forename: C
authors.initials: C
authors.lastname: LaLonde
authors.forename: D
authors.initials: D
authors.lastname: Zhu
authors.forename: R H
authors.initials: RH
authors.lastname: Demling
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism*; D004731:Endotoxins / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000201:enzymology / Q000293:injuries; D008168:Lung / Q000201:enzymology; D055370:Lung Injury; D012756:Sheep; D014115:Toxemia / Q000201:enzymology*
chemical_list: D004731:Endotoxins; C044101:endotoxin, Escherichia coli; D002374:Catalase