title: Regulation of transcription of the human T cell antigen receptor delta chain gene. A T lineage-specific enhancer element is located in the J delta 3-C delta intron.
abstract: We have defined transcriptional enhancing sequences inside the TCR-delta gene locus, using transient transfections with constructs containing DNA fragments cloned upstream to a reporter gene fused to a heterologous promoter. A 14-kb DNA region extending from the J delta 3 segment to 6 kb 3' to C delta was analyzed. We show the presence of positive regulatory sequences inside the J delta 3-C delta intron and have localized these sequences to two DNA fragments of approximately 300 and 258 bp. Analysis of cell specificity of the activation of such sequences demonstrates a T cell pattern for one of the two fragments. The nucleotide sequence of the T cell-specific element shows motifs sharing homology with previously described core enhancers.
authors.affiliation: Laboratoire d'Hématologie Moléculaire, Hôpital St. Louis, Paris, France.
authors.forename: J C
authors.initials: JC
authors.lastname: Bories
authors.forename: P
authors.initials: P
authors.lastname: Loiseau
authors.forename: L
authors.initials: L
authors.lastname: d'Auriol
authors.forename: C
authors.initials: C
authors.lastname: Gontier
authors.forename: A
authors.initials: A
authors.lastname: Bensussan
authors.forename: L
authors.initials: L
authors.lastname: Degos
authors.forename: F
authors.initials: F
authors.lastname: Sigaux
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D005796:Genes; D006801:Humans; D007438:Introns; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D015183:Restriction Mapping; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D046911:Macromolecular Substances; D011948:Receptors, Antigen, T-Cell