Mechanisms of inhibition of various cellular DNA and RNA polymerases by several flavonoids.
Four flavonoids (i.e., baicalein, quercetin, quercetagetin, and myricetin), known to be inhibitors of HIV-reverse transcriptase, have been shown to be more or less inhibitory to the activities of various cellular DNA and RNA polymerases. The degree of the inhibition varied depending on the combination of the flavonoid and the enzyme species: baicalein was moderately inhibitory to DNA polymerase gamma and E. coli DNA polymerase I; quercetin was strongly inhibitory to DNA polymerase beta and E. coli RNA polymerase and moderately inhibitory to DNA polymerase I; quercetagetin was a potent inhibitor for all of DNA polymerases alpha, beta, gamma, and I and RNA polymerase; myricetin was a strong inhibitor of DNA polymerases alpha and I and RNA polymerase. However, terminal deoxynucleotidyltransferase was virtually insensitive to inhibition by these flavonoids. The inhibition by the flavonoids was due to competition with the template.primer in the case of the DNA polymerases, whereas the inhibition was due to competition with the triphosphate substrate (GTP) in the case of RNA polymerase. The Ki values of these flavonoid inhibitors for DNA and RNA polymerases was determined.
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Laboratory of Viral Oncology, Aichi Cancer Center Research Institute.
Nakane
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D000818:Animals; D001667:Binding, Competitive; D004256:DNA Polymerase I / Q000037:antagonists & inhibitors; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors; D004258:DNA Polymerase III / Q000037:antagonists & inhibitors; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology; D005419:Flavonoids / Q000494:pharmacology*; D007700:Kinetics; D019384:Nucleic Acid Synthesis Inhibitors; D013379:Substrate Specificity
D005419:Flavonoids; D019384:Nucleic Acid Synthesis Inhibitors; D012321:DNA-Directed RNA Polymerases; D004256:DNA Polymerase I; D004257:DNA Polymerase II; D004258:DNA Polymerase III