Loss of yolk platelets and yolk glycoproteins during larval development of the sea urchin embryo.
The fate of the yolk platelets and their constituent yolk glycoproteins was studied in Strongylocentrotus purpuratus eggs and embryos cultured through the larval stage. Previous studies have shown that the yolk glycoproteins undergo limited proteolysis during early embryonic development. We present evidence that the yolk glycoproteins stored in the yolk platelets exist as large, disulfide-linked complexes that are maintained even after limited proteolysis have occurred. We provide additional evidence that acidification of the yolk platelet may activate a latent thiol protease in the yolk platelet that is capable of correctly processing the major yolk glycoprotein into the smaller yolk glycoproteins. Because we previously showed that these yolk glycoproteins are not catabolized during early embryonic development, it was of interest to study their fate during larval development. Using a specific polyclonal antibody to a yolk glycoprotein, we found that both yolk glycoproteins and the yolk platelets disappeared in feeding, Day 7, larval stage embryos, but that starvation did not significantly affect the levels of the yolk glycoproteins. We also found that the yolk glycoproteins reappeared in 30-day-old premetamorphosis larvae.
Scott
L B
LB
Department of Biochemistry and Molecular Biology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Leahy
P S
PS
Decker
G L
GL
Lennarz
W J
WJ
D000818:Animals; D004527:Egg Proteins / Q000302:isolation & purification / Q000502:physiology*; D004530:Egg Yolk; D004591:Electrophoresis, Polyacrylamide Gel; D004625:Embryo, Nonmammalian / Q000502:physiology*; D005260:Female; D006023:Glycoproteins / Q000302:isolation & purification / Q000502:physiology*; D015151:Immunoblotting; D007814:Larva / Q000502:physiology / Q000648:ultrastructure; D008854:Microscopy, Electron; D008970:Molecular Weight; D012617:Sea Urchins / Q000196:embryology / Q000254:growth & development*
D004527:Egg Proteins; D006023:Glycoproteins