title: Purification and characterization of tyrosylprotein sulfotransferase.
abstract: Tyrosylprotein sulfotransferase (TPST) is a Golgi membrane enzyme involved in the post-translational modification of secretory and membrane proteins. Here we describe the 140,000-fold purification of this enzyme from bovine adrenal medulla to apparent homogeneity and determine its substrate specificity. The key step in the purification was affinity chromatography on a substrate peptide to which the enzyme bound in the presence of nucleotide cosubstrate. TPST is a 54-50 kd integral membrane glycoprotein. The presence of sialic acid strongly suggests that within the Golgi complex, TPST is localized in the trans-most subcompartment. TPST was found to specifically sulfate tyrosine residues adjacent to acidic amino acids. These results define a major determinant for the specificity of protein sulfation in the trans Golgi.
authors.affiliation: Cell Biology Programme, European Molecular Biology Laboratory, Heidelberg, FRG.
authors.forename: C
authors.initials: C
authors.lastname: Niehrs
authors.forename: W B
authors.initials: WB
authors.lastname: Huttner
mesh_terms: D000313:Adrenal Medulla / Q000201:enzymology* / Q000648:ultrastructure; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000201:enzymology; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006056:Golgi Apparatus / Q000201:enzymology; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid; D012794:Sialic Acids / Q000032:analysis; D013379:Substrate Specificity; D015238:Sulfotransferases / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D012794:Sialic Acids; D015238:Sulfotransferases; C039079:protein-tyrosine sulfotransferase; D019158:N-Acetylneuraminic Acid