A simple method to create carotid endothelial laceration and acute platelet thrombus in vivo in guinea-pig.
A simple method, named 'clamp method', was developed to create carotid endothelial laceration and produce acute platelet thrombus in vivo in guinea-pig. This method used a hemostatic forceps to clamp common carotid artery of anesthetized guinea-pig at a tangent angle. Our study included two parts. The Part one demonstrated the procedures and effects of the 'clamp method': sixteen guinea-pigs were divided equally into control and heparin-treated groups. These two groups received the same clamping procedures except that the heparin-treated group was administrated with heparin (5,000U/kg) intravenously before clamping. One hour after the clamping, carotid arteries were resected and observed under scanning electron microscopy. The results showed that large carotid mural thrombi were formed in the control group with a mean surface area of 1.75 +/- 0.63 mm2 (M +/- SD, n = 8). In the heparin-treated group, the thrombus formation was prevented and linear endothelial lacerations were seen clearly. The Part two of our study identified the histologic nature of the thrombus produced by the 'clamp method': eight guinea-pigs received same procedures as that of control group in the Part one except that the carotid arteries were resected 5 min after performing the clamping. Four of the eight specimens were prepared for observation under transmission electron microscopy. The results showed that platelets adhered onto the subendothelial substances and formed platelet thrombi in the endothelial lacerations within 5 min after the carotid clamping.
Huang
Z S
ZS
Department of Internal Medicine, College of Medicine, National Taiwan University, Taipei, Republic of China.
Lee
T K
TK
Teng
C M
CM
Wang
C Y
CY
Huang
L H
LH
Chang
C H
CH
D000818:Animals; D001792:Blood Platelets / Q000473:pathology*; D020212:Carotid Artery Injuries; D003250:Constriction; D004730:Endothelium, Vascular / Q000293:injuries* / Q000648:ultrastructure; D005260:Female; D006168:Guinea Pigs; D006493:Heparin / Q000627:therapeutic use; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D013927:Thrombosis / Q000209:etiology* / Q000473:pathology / Q000517:prevention & control
D006493:Heparin