Imaging in scanning microscopes with slit-shaped detectors.
We consider the imaging in scanning microscopes employing point and slit-shaped detectors in both the bright-field and fluorescent mode. In particular we consider the three-dimensional aspects of the imaging and show inter alia that acceptable, albeit asymmetrical, images result from a slit detector system at low levels of defocus. The situation becomes worse as the defocus is increased although acceptable extended-focus and isometric images are still possible in some cases.
Wilson
T
T
University of Oxford, U.K.
Hewlett
S J
SJ
D007091:Image Processing, Computer-Assisted; D008433:Mathematics; D008853:Microscopy / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*