Enamel and dentin fluoride levels and fluorosis following single fluoride doses: a nuclear microprobe study.
Female rats were given single intraperitoneal doses of fluoride (F) (0, 4, 7, or 14 mg F/kg body weight). Plasma F levels returned to predose values within 24 h. Incisors from animals killed 35 or 70 days after the F doses had been given were analyzed for mineralization defects by microradiography, and for F and P concentrations by nuclear microprobe. At 35 days, all F-injected rats had enamel fluorosis. At 70 days, by which time the incisors would have been renewed nearly twice, fluorosis was still evident in the 14 mg/kg body weight group. The enamel and dentin F concentrations at each time point were proportional to the administered F doses that had been given weeks earlier. The F concentrations at 35 days were similar to those found at 70 days. The results support the hypothesis that, following pulse loading, F can be mobilized from the bone adjacent to the enamel organ and result in local F concentrations sufficiently large to adversely affect amelogenesis.
Angmar-Månsson
B
B
Department of Cariology, School of Dentistry, Karolinska Institute, Huddinge, Sweden.
Lindh
U
U
Whitford
G M
GM
D000818:Animals; D003743:Dental Enamel / Q000737:chemistry*; D003804:Dentin / Q000737:chemistry*; D004577:Electron Probe Microanalysis; D005260:Female; D005459:Fluorides / Q000008:administration & dosage / Q000032:analysis*; D005461:Fluorine / Q000032:analysis; D009050:Fluorosis, Dental / Q000209:etiology* / Q000378:metabolism; D007274:Injections, Intraperitoneal; D010758:Phosphorus / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013052:Spectrometry, X-Ray Emission; D013997:Time Factors
D010758:Phosphorus; D005461:Fluorine; D005459:Fluorides