[Isolation of plasmid DNA from Bifidobacterium].
Conditions for accumulation of the biomass and a procedure for isolation of plasmid DNA from bifidobacteria in microquantities were developed. It was shown that all the strains tested had 1 to 3 plasmids of different molecular weights electrophoretically detected. Relation between the detected plasmid DNA and bifidobacteria resistance to tetracycline and fusidin as well as utilization of some carbohydrates is discussed.
Donskikh
E E
EE
Tiurin
M V
MV
Goncharova
G I
GI
Shenderov
B A
BA
D001644:Bifidobacterium / Q000235:genetics*; D002498:Centrifugation; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D066298:In Vitro Techniques; D008970:Molecular Weight; D010957:Plasmids / Q000235:genetics*
D003470:Culture Media; D004269:DNA, Bacterial
Vydelenie plazmidnoĭ DNK iz bifidobakteriĭ.