Adherence of peritonitis-causing staphylococci to human peritoneal mesothelial cell monolayers.
The adherence of staphylococci to monolayers of human mesothelial cells was studied. Adherence of Staphylococcus aureus to mesothelial cell monolayers was 3.4-fold better than to plastic (P less than .01) whereas that of Staphylococcus epidermidis was 3.0-fold less than to plastic (P less than .01). Neither serum albumin nor gelatin inhibited staphylococcal binding. S. aureus adherence correlated with the amount of cell wall protein A (r = .63, P less than .05) but not with fibronectin binding; it was significantly inhibited by the addition of purified cell wall lipoteichoic acid (55% +/- 2.7%), teichoic acid (34.5% +/- 3.4%), and protein A (25.6% +/- 2.9%) but not peptidoglycan. Protein A- and teichoic acid-deficient mutants adhered less well than their parent strains, and encapsulated S. epidermidis adhere well to human monothelial cells. Staphylococcal binding may involve cell wall lipoteichoic acid, teichoic acid, and protein A.
Haagen
I A
IA
Laboratory of Microbiology, University of Utrecht Medical School, The Netherlands.
Heezius
H C
HC
Verkooyen
R P
RP
Verhoef
J
J
Verbrugh
H A
HA
D001422:Bacterial Adhesion; D001798:Blood Proteins / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D002478:Cells, Cultured; D006801:Humans; D008070:Lipopolysaccharides / Q000378:metabolism; D010457:Peptidoglycan / Q000378:metabolism; D010537:Peritoneum / Q000382:microbiology*; D010538:Peritonitis / Q000382:microbiology*; D013205:Staphylococcal Protein A / Q000378:metabolism; D013211:Staphylococcus aureus / Q000378:metabolism* / Q000648:ultrastructure; D013212:Staphylococcus epidermidis / Q000378:metabolism* / Q000648:ultrastructure; D013682:Teichoic Acids / Q000378:metabolism
D001798:Blood Proteins; D008070:Lipopolysaccharides; D010457:Peptidoglycan; D013205:Staphylococcal Protein A; D013682:Teichoic Acids; C009900:lipoteichoic acid