Antithrombotic activity of garlic: its inhibition of the synthesis of thromboxane-B2 during infusion of arachidonic acid and collagen in rabbits.
Rabbits were given collagen and arachidonic acid intravenously. Blood pressure, platelet counts, plasma thromboxane-B2 (TXB2) and plasma 6-keto-prostaglandin F1 alpha, (6-keto-PGF1 alpha) were determined. Both thrombogenic agents, upon infusion of a lethal dose, caused thrombocytopenia, indicative of in vivo platelet aggregation and hypotension. These changes were associated with an increase in plasma levels of TXB2 and 6-keto-PGF1 alpha measured by radioimmunoassay (RIA). Pretreatment of rabbits with an aqueous extract of garlic (500 mgkg) provided protection from thrombocytopenia and hypotension. Thromboxane-B2 synthesis was significantly reduced in animals pretreated with garlic and then injected with a lethal dose of either collagen or arachidonic acid. The amount of TXB2 synthesized in these animals was not sufficient to induce thrombocytopenia or hypotension. All animals pretreated with garlic were well protected against the effects of collagen or arachidonate infusion, and no apparent symptoms were observed in these animals. These observations indicate that garlic may be beneficial in the prevention of thrombosis.
Ali
M
M
Department of Biochemistry, Faculty of Science, Kuwait University.
Thomson
M
M
Alnaqeeb
M A
MA
al-Hassan
J M
JM
Khater
S H
SH
Gomes
S A
SA
D015121:6-Ketoprostaglandin F1 alpha / Q000097:blood*; D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology*; D001794:Blood Pressure; D003094:Collagen / Q000494:pharmacology*; D005260:Female; D005343:Fibrinolytic Agents; D005737:Garlic; D006973:Hypertension / Q000378:metabolism; D010946:Plants, Medicinal; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors; D010976:Platelet Count; D011817:Rabbits; D013921:Thrombocytopenia / Q000378:metabolism; D013929:Thromboxane B2 / Q000097:blood*
D001095:Arachidonic Acids; D005343:Fibrinolytic Agents; D010975:Platelet Aggregation Inhibitors; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D003094:Collagen