title: Virus quantification by immunofluorescence of cells grown in agar.
abstract: We have developed a method for assessing the number of infectious viral particles by measuring what we call fluorescence initiating units (FIV). The present work has been done with HIV, but the methods should be applicable to other viruses as well. Briefly described, cells are mixed with virus and then cast in an agar gel to block further transfer of virus. After a period of incubation sufficient to allow infected cells to express virus antigens, the percentage of infected cells is determined by indirect immunofluorescence.
authors.affiliation: Department of Virology, National Institute of Public Health, Oslo, Norway.
authors.forename: E
authors.initials: E
authors.lastname: Tjøtta
authors.forename: O
authors.initials: O
authors.lastname: Hungnes
authors.forename: B
authors.initials: B
authors.lastname: Grinde
mesh_terms: D002460:Cell Line; D005455:Fluorescent Antibody Technique; D006678:HIV / Q000254:growth & development*; D015488:HIV Antigens / Q000032:analysis; D006801:Humans; D014771:Virion / Q000254:growth & development
chemical_list: D015488:HIV Antigens