title: Ultrastructural alterations of right and left ventricular myocytes in tetralogy of Fallot.
abstract: We examined the ultrastructure of right and left ventricular myocardial biopsies obtained prior to cardiopulmonary bypass in fourteen patients undergoing total correction of tetralogy of Fallot (TOF). Twelve patients were undergoing primary one-stage repair and two patients had had a previous Blalock-Taussig shunt operation. The ages of the patients ranged from 3 to 35 years. In left ventricular (LV) myocytes, the sarcolemma often formed pouches filled with mitochondria and the muscle fibers showed a disoriented arrangement. In addition, the myofibrils showed hypercontraction and the Z-bands were thickened; the degree of the myofibril contraction was more severe than that in right ventricular (RV) myocytes. These ultrastructural findings are similar to those observed in myocytes under hypoxic conditions. The number of lysosomes in LV myocytes, but not in RV myocyte increased in the older patients. While we have not established a causal relationship between the ultrastructural findings and clinical features in our cases, our observations are consistent with the possibility that early total correction of TOF may be required to prevent the development of hypoxic changes and degeneration of heart muscle.
authors.affiliation: Department of Surgery, Kurume University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Isomura
authors.forename: K
authors.initials: K
authors.lastname: Hisatomi
authors.forename: H
authors.initials: H
authors.lastname: Inuzuka
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
authors.forename: N
authors.initials: N
authors.lastname: Hayashida
authors.forename: M
authors.initials: M
authors.lastname: Nishimi
authors.forename: H
authors.initials: H
authors.lastname: Maruyama
authors.forename: M
authors.initials: M
authors.lastname: Koga
authors.forename: K
authors.initials: K
authors.lastname: Kosuga
authors.forename: K
authors.initials: K
authors.lastname: Ohishi
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D001706:Biopsy; D002648:Child; D002675:Child, Preschool; D005260:Female; D006352:Heart Ventricles / Q000473:pathology; D006801:Humans; D008247:Lysosomes / Q000648:ultrastructure; D008297:Male; D008931:Mitochondria, Muscle / Q000648:ultrastructure; D009206:Myocardium / Q000648:ultrastructure*; D012508:Sarcolemma / Q000648:ultrastructure; D012518:Sarcomeres / Q000648:ultrastructure; D013771:Tetralogy of Fallot / Q000473:pathology*