title: Comparative study on the para-metabolic oxidation of phenytoin and decadeuteriophenytoin.
abstract: The in-vivo metabolic conversion of equal mixture of phenytoin and decadeuteriophenytoin to the para-hydroxy metabolite in rat was investigated in order to verify a possible role of an insertion or abstraction mechanisms in the hydroxylation process. Determination of kH/k2H values of urine samples at 2 h intervals for 24 h indicated that there was no isotope effect during in-vivo para-hydroxylation of phenytoin. This gives evidence of the arene oxide intermediacy possibly being the sole pathway for para-hydroxylation of phenytoin.
authors.affiliation: Department of Midicinal, Faculty of Pharmacy, University of Mansoura, Egypt.
authors.forename: M A
authors.initials: MA
authors.lastname: Moustafa
authors.forename: A A
authors.initials: AA
authors.lastname: el-Emam
authors.forename: H I
authors.initials: HI
authors.lastname: Subbagh
authors.forename: M K
authors.initials: MK
authors.lastname: el-Din
mesh_terms: D000818:Animals; D003903:Deuterium; D008401:Gas Chromatography-Mass Spectrometry; D006900:Hydroxylation; D008297:Male; D010084:Oxidation-Reduction; D010672:Phenytoin / Q000031:analogs & derivatives* / Q000378:metabolism*; D051381:Rats
chemical_list: C001419:hydroxyphenytoin; D010672:Phenytoin; D003903:Deuterium