title: Analysis, purification and properties of a 50,000-dalton membrane-associated phosphoprotein from human platelets.
abstract: Recently, the development of a monospecific antiserum against a 46,000/50,000-dalton membrane protein from human platelets which was stoichiometrically and reversibly phosphorylated in intact human platelets in response to vasodilators was reported. Using this antiserum, the subcellular distribution and the purification of this vasodilator-stimulated phosphoprotein (VASP) from human platelets has now been analysed. The VASP of human platelets is primarily a membrane-associated protein and can be purified to apparent homogeneity by salt extraction and sequential ion-exchange and dye-ligand chromatography with a purification factor of 1200 and a yield of 13%. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis under reducing and non-reducing conditions indicated that purified monomers of this VASP are linked by interchain disulphide bonding.
authors.affiliation: Medizinische Universitätsklinik, Labor für Klinische Biochemie, Würzburg, F.R.G.
authors.forename: M
authors.initials: M
authors.lastname: Halbrügge
authors.forename: U
authors.initials: U
authors.lastname: Walter
mesh_terms: D001792:Blood Platelets / Q000737:chemistry*; D015153:Blotting, Western; D002462:Cell Membrane / Q000737:chemistry; D002845:Chromatography; D002852:Chromatography, Ion Exchange; D003600:Cytosol / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000097:blood*; D008970:Molecular Weight; D010750:Phosphoproteins / Q000097:blood*; D010766:Phosphorylation; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D046911:Macromolecular Substances; D008565:Membrane Proteins; D010750:Phosphoproteins; D014665:Vasodilator Agents