Cyclosporin A and tissue antigen matching in bone transplantation. Fibular allografts studied in the dog.
We studied the mechanical, metabolic, and histologic properties of short-term nonvascularized cortical bone grafts in a canine fibular graft model. Sham operated nonvascularized autotransplanted and allotransplanted bones were compared. The allografts were performed between dog leukocyte antigen (DLA) class I and II matched; DLA class I and II mismatched; and cyclosporin A (CsA) treated, DLA class I and II mismatched animals. Cyclosporin was given for 1 month, and all the animals were followed for 3 months after surgery. Mechanical properties were investigated using standard torsional tests, metabolic kinetics were assessed using isotopic prelabeling techniques, and histomorphometric analysis of cross-sectional area properties and sequential fluorochrome labels were performed. Autografts were mechanically stronger and stiffer than all the types of allograft. CsA-treated, DLA-mismatched allografts performed better than matched allografts. These in turn were stronger than non-CsA-treated, mismatched allografts, which underwent nearly complete resorption. These relationships were preserved in the metabolic and histologic analyses. In this short-term animal study, although DLA matching resulted in a slight improvement in graft outcome, mismatched grafts in dogs receiving a short course of cyclosporin A fared even better.
Welter
J F
JF
Department of Orthopedics, Case Western Reserve University, Cleveland, OH 44106.
Shaffer
J W
JW
Stevenson
S
S
Davy
D T
DT
Field
G A
GA
Klein
L
L
Li
X Q
XQ
Zika
J M
JM
Goldberg
V M
VM
D000818:Animals; D001696:Biomechanical Phenomena; D016025:Bone Transplantation / Q000276:immunology; D003524:Cyclosporins / Q000627:therapeutic use*; D004285:Dogs; D005260:Female; D005360:Fibula / Q000378:metabolism / Q000637:transplantation; D006649:Histocompatibility Antigens / Q000276:immunology*; D015395:Histocompatibility Antigens Class I; D006650:Histocompatibility Testing; D008297:Male; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous
D003524:Cyclosporins; D006649:Histocompatibility Antigens; D015395:Histocompatibility Antigens Class I; C039692:histocompatibility antigen DLA