[Detection of antibodies against Toxoplasma gondii in human sera in immunoblot procedures].
Proteins of Toxoplasma lysate were separated by SDS-polyacrylamide gel electrophoresis with subsequent transfer to a nitrocellulose sheet by electrophoretic blotting. Patient human sera reacted predominant with protein fractions of Toxoplasma antigen of 94 kD and 35 kD. Human sera were tested by Toxoplasma-IgG ELISA and Immunofluorescence assay (IgG-IFAT, IgM-IFAT).
Koch
G
G
Institut für Medizinische Mikrobiologie, Medizinischen Akademie Magdeburg.
Wohlfahrth
A
A
Müller
W A
WA
D000818:Animals; D000913:Antibodies, Protozoan / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D014122:Toxoplasma / Q000276:immunology*
D000913:Antibodies, Protozoan
Nachweis von Antikörpern gegen Toxoplasma gondii in Humanseren im Immunoblotverfahren.