Epidermal growth factor stimulates DNA synthesis while inhibiting cell multiplication of A-431 carcinoma cells.
Epidermal growth factor (EGF) has been shown to inhibit the multiplication of the human epidermoid carcinoma cell line A-431. In the present report it is shown that, despite growth inhibition, EGF caused a marked synthesis of DNA and nonhistone proteins, without progression into mitosis. This event was associated with a retraction of the monolayer into colonies of cells. This suggests that the cell cycle of A-431 cells is controlled by two surface membrane signals: one generated by EGF stimulating the synthetic events of the G1 and S phases; a second signal, leading to progression into mitosis appears either not to be generated or to be inhibited by EGF.
Polet
H
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University of Illinois at Chicago, College of Medicine, Department of Pathology 60612.
D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D002455:Cell Division; D004247:DNA / Q000096:biosynthesis*; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D006801:Humans; D007399:Interphase; D008938:Mitosis; D014176:Protein Biosynthesis; D014407:Tumor Cells, Cultured
D004815:Epidermal Growth Factor; D004247:DNA