title: Identification of chromosomal antibiotic resistance genes in Streptococcus anginosus ("S. milleri").
abstract: Determinants encoding resistance to antibiotics, except penicillin, of 5 of 21 Streptococcus anginosus clinical isolates that we examined transferred by conjugation into Enterococcus faecalis and into 1 to 5 streptococcal recipients at frequencies of 10(-6) to 10(-8) transconjugants per donor. No R plasmids were detected in any wild-type strain. DNA homology was detected between chromosomal fragments and the following genes: aadE in 7 strains that were highly resistant to streptomycin, aph-3 in 8 strains that were highly resistant to kanamycin, ermB in 11 of 15 strains that were resistant to erythromycin, and tetM in 14 and tetO in 3 of the 18 strains that were resistant to tetracycline-minocycline. A Tn916-like structure was found in 10 of the 14 strains that carried the tetM gene.
authors.affiliation: Faculté Odontologique, Université Paris VII, France.
authors.forename: D
authors.initials: D
authors.lastname: Clermont
authors.forename: T
authors.initials: T
authors.lastname: Horaud
mesh_terms: D002875:Chromosomes / Q000502:physiology*; D003227:Conjugation, Genetic / Q000502:physiology; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004917:Erythromycin / Q000494:pharmacology; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000502:physiology; D013291:Streptococcus / Q000235:genetics*; D013753:Tetracycline Resistance / Q000235:genetics
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D004917:Erythromycin