Uterine epithelial cells in primary culture: a model system to study cell proliferation.
Guinea-pig uterine glandular epithelial cells were grown in primary culture. During the 4-day initial culture period, a 6.7 fold increase in DNA synthesis and a doubling time of approximately 30 hours were observed. Then the cells were submitted to serum depletion (60 hours) and the quiescent cells were stimulated with 15% fetal calf serum (FCS). The control cells were submitted to 1% heated and dextran-coated charcoal stripped FCS. In stimulated cells, the DNA synthesis increased and peaked between the 12th and 24th hour. In these cells, c-fos mRNAs increased rapidly, within 30 min., peaked at 75 min. (ratio to the control = 2.5), and returned to basal level within 90 min. These results prove that uterine epithelial cells in primary culture are able to respond to unspecific mitogen by both rapid expression of c-fos gene and DNA synthesis, suggesting that this cell culture system will be useful in studying the growth regulation in endometrium.
Jouvenot
M
M
INSERM U 198, Besançon, France.
Alkhalaf
M
M
Pellerin
I
I
Marechal
G
G
Royez
M
M
Adessi
G L
GL
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D008954:Models, Biological; D011519:Proto-Oncogenes; D014599:Uterus / Q000166:cytology* / Q000378:metabolism
D004247:DNA