Plasma versus bone marrow desipramine: a comparative study.
Correlation between plasma and bone marrow tricyclic antidepressants has not been studied before. Two groups of rabbits were given 10 and 20 mg of desipramine/kg body weight, respectively. Desipramine was administered to the animals once daily by mouth for 5 days. On the fifth day the animals were sacrificed and blood and bone marrow samples were collected and analyzed using a high performance liquid chromatographic (HPLC) method. Data showed that a correlation exists between bone marrow and blood desipramine. The bone marrow desipramine concentration increased as its blood levels increased. The average ratio of bone marrow to blood desipramine +/- S.D. (standard deviation) in both dosage groups was 37.2 +/- 4.46 with a range of 30.99-44.82. This investigation is promising and shows that bone marrow could be used as an alternative tissue in the absence of a suitable blood sample.
Winek
C L
CL
Allegheny County Department of Laboratories, Pittsburgh, PA 15219.
Westwood
S E
SE
Wahba
W W
WW
D000818:Animals; D001853:Bone Marrow / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003891:Desipramine / Q000032:analysis* / Q000097:blood; D011817:Rabbits
D003891:Desipramine