The cloning and expression of a gene encoding haemolytic activity from the fish pathogen Renibacterium salmoninarum.
A gene encoding haemolytic activity from Renibacterium salmoninarum (strain PPD) was cloned into Escherichia coli using the cosmid vector pHC79, and subsequently subcloned on a 1.6 kbp SAlI fragment into pBR328. Southern blot hybridisation revealed that a homologous sequence is found in other strains of R. salmoninarum.
Evenden
A J
AJ
Department of Biological Sciences, Polytechnic South West, Plymouth, U.K.
Gilpin
M L
ML
Munn
C B
CB
D000818:Animals; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003360:Cosmids; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D006093:Gram-Positive Asporogenous Rods / Q000235:genetics* / Q000472:pathogenicity; D006460:Hemolysin Proteins / Q000235:genetics*; D006461:Hemolysis; D015183:Restriction Mapping; D012487:Salmonidae / Q000382:microbiology; D012689:Sequence Homology, Nucleic Acid
D004269:DNA, Bacterial; D006460:Hemolysin Proteins