[Study of the action of immunomodulator T-activin on electric properties of plasma membranes of thymus cells by the use of fluorescent probes].
T-activin alters the electric properties of plasmatic membranes of the T-activin treated suspension of mouse (CBAX X057B1) F1 thymic cells. These alterations were registered by means of negative charged fluorescent probe l-anilino-naphthalene-8-sulfonate (ANS) and positive charged 4-(p-dimethylaminostyryl)-1-methylpyridinium (DSM) by measurement of their fluorescence in the individual thymic cell. Apparently, T-activin leads to depolarization of transmembrane potential on plasmatic membrane of thymic cells. It seems that action of T-activin is similar to the effect of polycations, which alter cells ionic streams during lymphocyte activation. However, there is a version, that T-activin acts like neuraminidase, but it is activity less expressed.
Petrov
V A
VA
Sinegubov
A V
AV
Sokolova
E V
EV
Glukhova
N V
NV
Smeianov
V V
VV
Koval'chuk
L V
LV
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects* / Q000502:physiology; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009439:Neuraminidase / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects; D013949:Thymus Extracts / Q000494:pharmacology*; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects*
D000276:Adjuvants, Immunologic; D005456:Fluorescent Dyes; D010455:Peptides; D013949:Thymus Extracts; C036191:T-activin; D009439:Neuraminidase
Izuchenie deĭstviia immunomoduliatora T-aktivina na élektricheskie svoĭstva plasmaticheskoĭ membrany kletok timusa s pomoshch'iu fliuorestsentnykh zondov.