Characterization of in vivo immunoliposome targeting to pulmonary endothelium.
Two rat monoclonal antibodies, 34A and 201B, which specifically bind to a surface glycoprotein (gp112) of the pulmonary endothelial cell surface, have been coupled to unilamellar liposomes of approximately 0.25 microns in diameter. The 34A- and 201B-liposomes (monoclonal antibodies 273-34A and 411-201B, respectively), but not antibody-free liposomes and liposomes coupled to 14, a nonspecific monoclonal antibody, accumulate efficiently (approximately 30% injected dose) in the lung of mice which have been injected via the tail vein. Immunoliposome targeting to lung is demonstrated both by using a 125I-labeled lipid marker and an entrapped water-soluble marker. Lung accumulation of 34A-liposomes is completely blocked by a preincubation of free antibody 34A, but not antibody 14, indicating that the immunoliposome accumulation at the target site is immunospecific. Time course studies have revealed that 34A-liposomes bind to lung antigens within 1 min after injection, indicating that the target binding takes place during the first few passages of immunoliposomes through the lung capillary bed. Unbound immunoliposomes are taken up by liver and spleen within 3-5 min after injection. The level of lung accumulation increases significantly as the protein:lipid ratio of the immunoliposome increases. Approximately 50% of injected dose is accumulated in lung for 34A-liposomes, with an average of 935 antibody molecules per liposome. Immunoliposomes of larger size accumulate in lung more significantly than those of smaller size. Injection with higher doses also enhances the level of lung accumulation.(ABSTRACT TRUNCATED AT 250 WORDS)
Maruyama
K
K
Department of Biochemistry, University of Tennessee, Knoxville 37996-0840.
Holmberg
E
E
Kennel
S J
SJ
Klibanov
A
A
Torchilin
V P
VP
Huang
L
L
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D004337:Drug Carriers; D004727:Endothelium / Q000378:metabolism; D007074:Immunoglobulin G / Q000276:immunology; D007444:Inulin / Q000008:administration & dosage / Q000493:pharmacokinetics; D007457:Iodine Radioisotopes; D008081:Liposomes / Q000737:chemistry / Q000493:pharmacokinetics*; D008168:Lung / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010316:Particle Size; D051381:Rats; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D004337:Drug Carriers; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D008081:Liposomes; D007444:Inulin