Outer membrane proteins of Bacteroides fragilis grown in vivo.
The outer membrane protein (OMP) profiles of four different strains of Bacteroides fragilis, as determined by Coomassie blue stained polyacrylamide gels, were compared after growth in broth culture and in the mouse peritoneal cavity. There was no induction of the expression of large quantities of novel OMP after growth in vivo. Mouse immunoglobulin G and albumin were associated with the bacterial OMP, but could be removed by washing.
Patrick
S
S
Department of Microbiology and Immunobiology, Queen's University, Belfast, U.K.
Lutton
D A
DA
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000096:biosynthesis*; D001441:Bacteroides fragilis / Q000032:analysis / Q000254:growth & development / Q000378:metabolism*; D003470:Culture Media; D015194:Diffusion Chambers, Culture; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000032:analysis; D051379:Mice; D010529:Peritoneal Cavity; D012709:Serum Albumin / Q000032:analysis
D001425:Bacterial Outer Membrane Proteins; D003470:Culture Media; D007074:Immunoglobulin G; D012709:Serum Albumin