Structure of polysaccharide-peptidoglycan complex from the cell wall of Lactobacillus casei YIT9018.
The isolation and analysis of the polysaccharide-peptidoglycan complexes of Lactobacillus casei YIT9018 are presented. Two polysaccharide-peptidoglycan complexes, PS-PG1 and PS-PG2, were solubilized from the heat-killed cell by treatment with N-acetylmuramidase. PS-PG1 was composed of glucose, rhamnose, and small amount of galactose and glucosamine. PS-PG2 was composed of glucose, rhamnose, galactosamine, and glucosamine. The ratio by weight of these fractions was about 1:8. PS-PG2 was analyzed in detail. Smith degradation and deamination of this complex yielded oligosaccharide units. The results of methylation analysis of these units and intact PS-PG2 led to the most probable structure of PS-PG2: (formula; see text)
Nagaoka
M
M
Yakult Central Institute for Microbiological Research, Tokyo.
Muto
M
M
Nomoto
K
K
Matuzaki
T
T
Watanabe
T
T
Yokokura
T
T
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry*; D002850:Chromatography, Gel; D003641:Deamination; D007778:Lactobacillus / Q000032:analysis*; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000737:chemistry* / Q000302:isolation & purification; D011134:Polysaccharides / Q000737:chemistry*
D010457:Peptidoglycan; D011134:Polysaccharides