title: Neuron-specific membrane glycoproteins promoting neurite fasciculation in Aplysia californica.
abstract: We have generated a library of mouse monoclonal antibodies against membrane proteins of the nervous system of the marine snail Aplysia californica. Two of these antibodies, 4E8 and 3D9, recognize a group of membrane glycoproteins with molecular masses of 100-150 kD. We have called these proteins ap100, from the molecular mass of the most abundant species. Based on Western blots, these proteins appear to be specific for the nervous system. They are enriched in the neuropil of central nervous system ganglia, and are present on the surface of neurites and growth cones of neurons in culture. They are not expressed on the surface of nonneuronal cells. Staining of living cells with fluorescently labeled mAb demonstrates that the epitope(s) are on the outside of the cell. The antibodies against the proteins defasciculate growing axons and alter the morphology of growth cones, but affect much less adhesion between neuritic shafts. In addition, the level of expression of these molecules appears to correlate with the degree of fasciculation of neurites. These observations suggest that the ap100 proteins are cell adhesion molecules that play a role in axon growth in the nervous system of Aplysia. The fact that they are enriched in the neuropil and possibly in varicosities suggest that they may also be relevant for the structure of mature synapses.
authors.affiliation: Howard Hughes Medical Institute, College of Physicians and Surgeons, Columbia University, New York 10032.
authors.forename: F
authors.initials: F
authors.lastname: Keller
authors.forename: S
authors.initials: S
authors.lastname: Schacher
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001048:Aplysia; D001369:Axons / Q000502:physiology* / Q000648:ultrastructure; D015153:Blotting, Western; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D008562:Membrane Glycoproteins / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D016253:Microscopy, Immunoelectron; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D009474:Neurons / Q000502:physiology*
chemical_list: D000911:Antibodies, Monoclonal; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins