Effects of amino acids on acute alcohol intoxication in mice--concentrations of ethanol, acetaldehyde, acetate and acetone in blood and tissues.
Condensation reactions between some SH-amino acids (L-and D-cysteine 1%) and acetaldehyde (50 microM) were studied in vitro experiment. In the aqueous solution, free acetaldehyde was reduced to 41.3% by L-cysteine and to 36.4% by D-cysteine. In the reaction with human blood medium, after the medium was deproteinized with perchloric acid reagent, acetaldehyde was reduced to 47.0% by L-cysteine and to 43.8% by D-cysteine. D-Cysteine appears to have great stability of reacting acetaldehyde. In vitro experiment reactability for D-cysteine exhibited 3-8% higher than that for L-cysteine. Next, effects of some amino acids on alcohol metabolism were studied in male ICR mice. The animals were given ethanol through a gastric catheter at a dose of 2 g/kg and they were intraperitoneally injected L-cysteine (300 mg/kg), D-cysteine (300 mg/kg), L-alanine (300 mg/kg) and control (saline), respectively in the period of one hour before the injection of ethanol. Blood and tissues samples were analyzed for ethanol, acetaldehyde, acetate and acetone during alcohol intoxication in mice by head space gas chromatography. In the groups administered D-cysteine and L-cysteine, the mice showed a definitely faster oxidation and disappearance of ethanol. Especially in the D-cysteine group, ethanol levels in blood, liver and brain remained lower than that in the other groups (p less than 0.01). Acetaldehyde levels in blood, liver and brain remained low by L-cysteine. Ethanol metabolites during alcohol oxidation by chemical reactabilities of L- and D-cysteine showed different distribution in the mice, respectively. In the mice received L-alanine, acetate and acetone levels in blood, liver and brain were distinctly reduced (p less than 0.01). L-Alanine is reported to supply an abundance of pyruvic acid that performs the NAD-generating system. NAD produced is introduced to alcohol metabolism and the TCA cycle. It was thus presumed that the L- or/and D-cysteine, and L-alanine was effective in acute alcohol intoxication by heavy drinking.
Tsukamoto
S
S
Department of Legal Medicine, Nihon University School of Medicine.
Kanegae
T
T
Nagoya
T
T
Shimamura
M
M
Mieda
Y
Y
Nomura
M
M
Hojo
K
K
Okubo
H
H
D000079:Acetaldehyde / Q000097:blood / Q000378:metabolism*; D000085:Acetates / Q000097:blood / Q000378:metabolism*; D000096:Acetone / Q000097:blood / Q000378:metabolism*; D000409:Alanine / Q000494:pharmacology; D000435:Alcoholic Intoxication / Q000378:metabolism* / Q000517:prevention & control; D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D003545:Cysteine / Q000494:pharmacology; D000431:Ethanol / Q000097:blood / Q000378:metabolism*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR
D000085:Acetates; D000596:Amino Acids; D000096:Acetone; D000431:Ethanol; D000079:Acetaldehyde; D003545:Cysteine; D000409:Alanine