title: The behaviour of cells from the distal tips of quail wing buds when grafted back into chick wings after micromass culture.
abstract: In high density culture, cells from distal tips of developing limb buds differentiate into a continuous cartilage sheet, rich in type II collagen. When grafted back into limb buds, cells cultured for a short time differentiate into cartilage and a wide range of other connective tissues, whereas cells taken from older cultures give rise only to cartilage and perichondrium. Grafts placed distally give rise to more cell types than grafts placed proximally. The results strongly suggest that chondrogenesis in culture is the result of removing the signals that pattern differentiation within the limb bud.
authors.affiliation: Department of Anatomy and Developmental Biology, University College and Middlesex School of Medicine, London, U.K.
authors.forename: C P
authors.initials: CP
authors.lastname: Cottrill
authors.forename: A
authors.initials: A
authors.lastname: Crawley
authors.forename: C
authors.initials: C
authors.lastname: Tickle
mesh_terms: D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000196:embryology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D002642:Chick Embryo; D003238:Connective Tissue / Q000196:embryology*; D003239:Connective Tissue Cells; D003370:Coturnix; D013997:Time Factors; D014183:Transplantation, Heterologous; D014921:Wings, Animal / Q000166:cytology / Q000196:embryology* / Q000637:transplantation