Static and scanning array detection in capillary electrophoresis-mass spectrometry.
An array detection system based on position- and time-resolved ion counting was evaluated for capillary electrophoresis-mass spectrometry using continuous-flow fast atom bombardment and a liquid-junction coupling. Peptides with molecular masses up to 3200 were measured. A 100-1000-fold improvement over conventional detection was demonstrated by applying the array detector in scanning and static modes. Absolute detection limits in the range 1-5 fmol are achievable.
Reinhoud
N J
NJ
Division of Analytical Chemistry, Centre for Bio-Pharmaceutical Sciences, Leiden, The Netherlands.
Schröder
E
E
Tjaden
U R
UR
Niessen
W M
WM
ten Noever de Brauw
M C
MC
van der Greef
J
J
D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D008970:Molecular Weight; D001615:beta-Endorphin / Q000032:analysis
D001615:beta-Endorphin