An improved method for quantitative determination of urinary porphyrins by use of second-derivative spectroscopy.
An improved assay for quantification of urinary porphyrins by use of second-derivative spectroscopy is described. A new method for calculation of the porphyrin concentration is developed and the whole procedure is computerized. Acidified urine samples can be assayed within a few minutes by using this method. Precision and recoveries for both uro- and coproporphyrin are good. The method is presented as a very fast and accurate assay for the screening and quantification of urinary porphyrins.
van de Giessen
A W
AW
Clinical Chemistry Department, Military Hospital Dr. A. Mathijsen, Utrecht, The Netherlands.
van Wijk
E M
EM
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003306:Coproporphyrins / Q000652:urine; D006801:Humans; D011166:Porphyrins / Q000652:urine*; D012044:Regression Analysis; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013057:Spectrum Analysis / Q000379:methods; D014578:Uroporphyrins / Q000652:urine
D003306:Coproporphyrins; D011166:Porphyrins; D014578:Uroporphyrins