Matrix solid phase dispersion isolation and liquid chromatographic determination of five benzimidazole anthelmintics in fortified beef liver.
A multiresidue method for isolation and liquid chromatographic determination of 5 benzimidazole anthelmintics (thiabendazole, oxfendazole, mebendazole, albendazole, and fenbendazole) in beef liver tissue is presented. Blank or benzimidazole-fortified liver samples (0.5 g) were blended with octadecylsilyl derivatized silica packing material (C18, 18% load, endcapped, 2 g). A column made from the C18/liver matrix was first washed with hexane (8 mL), following which the benzimidazoles were eluted with acetonitrile. The acetonitrile extract was then passed through an activated alumina column. The eluate contained benzimidazole analytes that were free from interfering compounds as determined by UV detection (photodiode array, 290 nm). Correlation coefficients of standard curves for individual benzimidazoles isolated from fortified samples, using internal standardization, were linear (0.996 +/- 0.002 to 0.999 +/- 0.001) with average relative percentage recoveries from 62.0 +/- 6.7 to 86.8 +/- 8.6% for the concentration range (100-3200 ng/g) examined. The interassay variability was 7.0 +/- 4.1 to 12.9 +/- 10.2% with an intra-assay variability from 2.2 to 4.0%.
Long
A R
AR
Louisiana State University School of Veterinary Medicine, Department of Veterinary Physiology, Pharmacology, and Toxicology, Baton Rouge 70803.
Malbrough
M S
MS
Hsieh
L C
LC
Short
C R
CR
Barker
S A
SA
D000818:Animals; D000871:Anthelmintics / Q000032:analysis* / Q000302:isolation & purification; D001562:Benzimidazoles / Q000032:analysis* / Q000302:isolation & purification; D002417:Cattle; D002853:Chromatography, Liquid; D004350:Drug Residues / Q000032:analysis; D005506:Food Contamination / Q000032:analysis*; D008099:Liver / Q000737:chemistry*; D008460:Meat / Q000032:analysis*
D000871:Anthelmintics; D001562:Benzimidazoles