title: Flow cytometric DNA content analysis in primary lung cancer: comparison of results from fresh and paraffin-embedded specimens.
abstract: Tumor DNA content was determined by flow cytometry in 24 surgically resected specimens from patients with primary lung cancer to compare results from fresh and paraffin-embedded specimens. We found a good correlation in DNA indices between fresh and paraffin-embedded specimens (r = .978), although one tumor with DNA near-diploidy was mistaken as DNA diploid in paraffin-embedded specimens. In the measurements of paraffin-embedded specimens, eight of 24 tumors were DNA multiploid and showed DNA content heterogeneity. These results indicate that DNA content analysis of paraffin-embedded specimens is helpful because it makes it easy to analyze multiple specimens from different parts of the tumor.
authors.affiliation: First Department of Medicine, School of Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Isobe
authors.forename: H
authors.initials: H
authors.lastname: Miyamoto
authors.forename: K
authors.initials: K
authors.lastname: Inoue
authors.forename: M
authors.initials: M
authors.lastname: Shimizu
authors.forename: T
authors.initials: T
authors.lastname: Endo
authors.forename: S
authors.initials: S
authors.lastname: Mizuno
authors.forename: Y
authors.initials: Y
authors.lastname: Kawakami
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D010232:Paraffin; D011003:Ploidies
chemical_list: D004273:DNA, Neoplasm; D010232:Paraffin