T cell responses in a sporozoite-immunized human volunteer and a chimpanzee.
We have recently investigated the specificity of T cells induced in a human volunteer and a chimpanzee immunized by multiple exposures to the bites of large numbers of malaria-infected mosquitoes. T cell lines and clones have been obtained from a human volunteer immunized with Plasmodium falciparum sporozoites. These CD4+ T cell clones specifically recognize the native circumsporozoite (CS) protein expressed on sporozoites, as well as bacteria- and yeast-derived recombinant falciparum CS proteins. The epitope recognized by the sporozoite specific human T cells mapped to the 5' repeat region of the CS protein and was contained in the NANPNVDPNANP sequence. A T cell line has also been isolated from PBL obtained from a chimpanzee immunized by multiple exposures to the bits of P. vivax infected mosquitoes. The CS-specific chimpanzee T cells were used to identify a T cell epitope within a repeat region of the P. vivax CS protein.
Nardin
E H
EH
New York University School of Medicine, Department of Medical and Molecular Parasitology, NY.
D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry / Q000276:immunology*; D002999:Clone Cells; D006801:Humans; D007114:Immunization; D008969:Molecular Sequence Data; D002679:Pan troglodytes; D010446:Peptide Fragments / Q000276:immunology; D010963:Plasmodium falciparum / Q000276:immunology*; D010966:Plasmodium vivax / Q000276:immunology*; D015800:Protozoan Proteins; D013601:T-Lymphocytes / Q000276:immunology*
D000953:Antigens, Protozoan; D010446:Peptide Fragments; D015800:Protozoan Proteins; C041595:circumsporozoite protein, Protozoan