title: Immunological studies with a monoclonal antibody suggest a different conformation of neurophysin in parvicellular neurons of rat hypothalamus.
abstract: A monoclonal antibody (mAb L6) to a carcinoma surface antigen has previously been shown to recognize neurophysins (NP), proteins associated with oxytocin and vasopressin. L6-reactivity in rat hypothalamus was confined to magnocellular neuronal systems. No staining was detected in parvicellular suprachiasmatic or paraventricular systems. mAb L6 immunoprecipitated vasopressin-neurophysin only under reducing conditions, and detected it in Western blots only after gel-renaturation and electroblotting in basic buffer. These findings suggest L6-reactivity to NP is conformation-sensitive, and imply NP expression in a unique configurational form in hypothalamic parvicellular systems.
authors.affiliation: Department of Neurology, Oregon Health Sciences University, Portland 97201.
authors.forename: G
authors.initials: G
authors.lastname: Nilaver
authors.forename: L C
authors.initials: LC
authors.lastname: Rosenbaum
authors.forename: H H
authors.initials: HH
authors.lastname: Van Tol
authors.forename: E M
authors.initials: EM
authors.lastname: Shannon
authors.forename: H M
authors.initials: HM
authors.lastname: Hagman
authors.forename: E A
authors.initials: EA
authors.lastname: Zimmerman
authors.forename: E A
authors.initials: EA
authors.lastname: Neuwelt
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D007031:Hypothalamus / Q000737:chemistry / Q000166:cytology*; D015151:Immunoblotting; D007150:Immunohistochemistry; D008297:Male; D008473:Median Eminence / Q000166:cytology; D008970:Molecular Weight; D009474:Neurons / Q000166:cytology*; D009481:Neurophysins / Q000032:analysis*; D051381:Rats
chemical_list: D000911:Antibodies, Monoclonal; D009481:Neurophysins