Myeloid calcium binding proteins: expression in the differentiated HL-60 cells and detection in sera of patients with connective tissue diseases.
A differentiation antigen induced in 1,25-dihydroxyvitamin D3 (VD3)-treated HL-60 cells was identified as being comprised of the myeloid calcium binding proteins CaBP-p8 and -p14 by determining its amino acid and DNA sequence. Northern blot analysis using a DNA fragment of the gene encoding p14 as a probe indicated that the gene was not expressed in undifferentiated HL-60 cells but transcribed starting on day 1 after VD3 treatment. The level of p 14 mRNA reached a peak on day 2, then declined, and little mRNA remained on day 10, indicating that the p14 gene is activated once and then inactivated during HL-60 differentiation due to VD3. In contrast, thymidylate synthase (TSase) mRNA was present in undifferentiated HL-60 cells but disappeared quickly after VD3 treatment. Both p8 and p14 of CaBP were found at elevated levels in sera of some patients with connective tissue diseases [highly elevated in rheumatoid arthritis (RA), Sjogren's syndrome (SjS), systemic lupus erythematosus (SLE), and progressive systemic sclerosis (PSS), and moderately in polymyositis or dermatomyositis (PM/DM) and mixed connective tissue disease (MCTD)]. These results were in sharp contrast with the finding that p14 is always at a highly elevated level but little p8 is present in the sera of cystic fibrosis (CF) patients [Bruggen et al. (1988) Nature 331, 570).
Kuruto
R
R
Laboratory of Microbiology, University of Shizuoka School of Food and Nutritional Science.
Nozawa
R
R
Takeishi
K
K
Arai
K
K
Yokota
T
T
Takasaki
Y
Y
D000943:Antigens, Differentiation / Q000276:immunology; D002117:Calcitriol / Q000494:pharmacology; D002135:Calcium-Binding Proteins / Q000276:immunology / Q000378:metabolism*; D040501:Calgranulin A; D040502:Calgranulin B; D002454:Cell Differentiation; D003240:Connective Tissue Diseases / Q000235:genetics / Q000378:metabolism*; D015342:DNA Probes; D006801:Humans; D009504:Neutrophils / Q000276:immunology; D011233:Precipitin Tests; D012333:RNA, Messenger / Q000378:metabolism; D013940:Thymidylate Synthase / Q000235:genetics; D014407:Tumor Cells, Cultured
D000943:Antigens, Differentiation; D002135:Calcium-Binding Proteins; D040501:Calgranulin A; D040502:Calgranulin B; D015342:DNA Probes; D012333:RNA, Messenger; D013940:Thymidylate Synthase; D002117:Calcitriol