Isoelectric focusing herpes simplex virus-gB overlay study in brainstem encephalitis.
We report a case of herpetic brainstem encephalitis (HBE) retrospectively diagnosed in adult patient. Conventional immunovirological studies failed to disclose the etiology of this patient's affection. An isoelectric focusing-antigen overlay (IEF-O) technique showed that the target of one of the four cerebrospinal fluid oligoclonal bands was herpes simplex virus (HSV)-1 glycoprotein B, indicating a specific anti-HSV immunoresponse restricted to the CNS. IEF-O may represent a useful support for in vivo diagnosis of HBE.
Martino
G V
GV
Neurology Clinic IV, Milan University, IRCCS H. San Raffaele, Italy.
Grimaldi
L M
LM
Franciotta
D M
DM
Piccolo
G
G
Brustia
R
R
Minali
L
L
Melzi d'Eril
G V
GV
D000328:Adult; D001933:Brain Stem / Q000276:immunology; D003937:Diagnosis, Differential; D004660:Encephalitis / Q000175:diagnosis* / Q000276:immunology; D005260:Female; D006561:Herpes Simplex / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D007136:Immunoglobulins / Q000134:cerebrospinal fluid; D007525:Isoelectric Focusing; D043025:Oligoclonal Bands; D014759:Viral Envelope Proteins / Q000134:cerebrospinal fluid*
D007136:Immunoglobulins; D043025:Oligoclonal Bands; D014759:Viral Envelope Proteins; C040495:glycoprotein B, Simplexvirus