title: Effects of (MET-5) enkephalin on the electrically-evoked mechanical responses in longitudinal and circular strips of the cat terminal ileum.
abstract: In longitudinal and circular strips from cat terminal ileum field electrical stimulation at a frequency of 2 Hz evoked contractile responses. Stimulation at frequencies of 10 or 30 Hz elicited contractions of the longitudinal muscle and relaxations of the circular strips. (Met-5) enkephalin (1 nM) naloxone-dependently reduced the contractile and increased the inhibitory responses. Atropine (3 microM) converted the contractile responses to slight relaxations and potentiated the inhibitory responses. After atropine (3 microM) and guanethidine (50 microM) both longitudinal and circular strips responded to electrical stimulation with relaxations. In atropine-pretreated strips (Met-5) enkephalin was effective only in the circular strips, increasing the inhibitory responses. In contrast, after atropine and guanethidine (Met-5) enkephalin decreased these inhibitory responses. In unstimulated strips (Met-5) enkephalin failed to change the responses to acetylcholine and noradrenaline. It is concluded that (Met-5) enkephalin reduces the excitatory cholinergic components of the electrically-evoked responses in both longitudinal and circular strips as well as the excitatory adrenergic and the inhibitory non-adrenergic, non-cholinergic components of the responses in the circular strips by acting presynaptically. Demonstration of (Met-5) enkephalin-like immunoreactivity showed immunostaining in nerves of the myenteric plexus and in nerve fibers between the smooth muscle cells suggesting that (Met-5) enkephalin effects could be also of physiological significance.
authors.affiliation: Institute of Physiology, Bulgarian Academy of Sciences, Sofia.
authors.forename: R
authors.initials: R
authors.lastname: Radomirov
authors.forename: N
authors.initials: N
authors.lastname: Pencheva
authors.forename: K
authors.initials: K
authors.lastname: Venkova
authors.forename: M
authors.initials: M
authors.lastname: Davidoff
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001696:Biomechanical Phenomena; D002415:Cats; D004558:Electric Stimulation; D004744:Enkephalin, Methionine / Q000494:pharmacology*; D006145:Guanethidine / Q000494:pharmacology; D007082:Ileum / Q000187:drug effects; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects*; D009270:Naloxone / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology
chemical_list: D009270:Naloxone; D004744:Enkephalin, Methionine; D001285:Atropine; D000109:Acetylcholine; D009638:Norepinephrine; D006145:Guanethidine