Polymeric dimethylaminopyridinium reagents for derivatization of weak nucleophiles in high-performance liquid chromatography-ultraviolet/fluorescence detection.
This paper introduces a novel polymeric dimethylaminopyridinium 9-fluorenyl-methoxycarbonyl reagent for off-line derivatizations of weak nucleophiles in high-performance liquid chromatography. The method of synthesis and characterization of the polymeric reagent via loading determinations is presented and discussed. Derivatization conditions (solvent, time, and temperature) for primary and secondary alcohols were optimized. As one application, off-line derivatizations of 2-chloro-1-propanol, a potential carcinogen in foodstuffs, were carried out with this polymeric reagent with single-blind and standard addition techniques. A specific sample treatment procedure was also developed. The accuracy and precision of the method were determined and data were statistically evaluated.
Gao
C X
CX
Department of Chemistry, Northeastern University, Boston, MA 02115.
Krull
I S
IS
D000433:1-Propanol / Q000032:analysis; D015761:4-Aminopyridine / Q000031:analogs & derivatives* / Q000032:analysis; D000215:Acylation; D000438:Alcohols / Q000032:analysis; D002728:Chlorohydrins / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000431:Ethanol / Q000032:analysis; D005449:Fluorenes / Q000032:analysis; D005453:Fluorescence; D011108:Polymers / Q000032:analysis; D011726:Pyridinium Compounds / Q000032:analysis; D013696:Temperature; D013997:Time Factors; D014466:Ultraviolet Rays
D000438:Alcohols; D002728:Chlorohydrins; D005449:Fluorenes; D011108:Polymers; D011726:Pyridinium Compounds; D000431:Ethanol; D000433:1-Propanol; D015761:4-Aminopyridine; C053204:2-chloro-1-propanol