[Influences of 3,4,5-trihydroxystibene-3-beta-mono-D-glucoside on beat rate and injury of cultured newborn rat myocardial cells].
3,4,5-Trihydroxystibene-3-beta-mono-D-glucoside (PD) from Polygonum cuspidatum 0.05-0.45 mmol/L increased beat rate of myocardial cells from 100% to 112-220%. This action of PD 0.15 mmol/L was agonized by nisoldipine 0.1, propranolol 10 and phentolamine 1 mumol/L. PD (0.05, 0.15 mmol/L) decreased the lactate dehydrogenase (LDH) amount liberated by myocardial cells, from 98 +/- 11 IU/ml of deprivation of oxygen and glucose (DOG) to 55 +/- 16, 68 +/- 14 IU/ml (P less than 0.01) 6 h after injury of DOG; 77 +/- 9, 80 +/- 6 IU/ml (P less than 0.01) 9 h after injury of DOG. PD 0.15 mmol/L decreased the LDH amount liberated by myocardial cells, from 311 +/- 34 to 231 +/- 42 IU/ml (P less than 0.05) 9 h after injury of chlorpromazine (CPZ). It is suggested that PD can protect myocardial cells injured by DOG and CPZ.
Luo
S F
SF
Department of Pharmacology, First Military Medical College, Guangzhou, China.
Yu
C L
CL
Zhang
P W
PW
D000818:Animals; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005960:Glucosides / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009206:Myocardium / Q000166:cytology*; D015737:Nisoldipine / Q000494:pharmacology; D010646:Phentolamine / Q000494:pharmacology; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D013267:Stilbenes / Q000494:pharmacology*
D004365:Drugs, Chinese Herbal; D005960:Glucosides; D010975:Platelet Aggregation Inhibitors; D013267:Stilbenes; D015737:Nisoldipine; D011433:Propranolol; D007770:L-Lactate Dehydrogenase; C058229:polydatin; D010646:Phentolamine