[Use of lectin histochemistry method in the characterization of biopolymer glycosylation processes in duodenal gland cells].
Potentialities of lectin histochemistry method for analysis of glycosylation processes within secretory epitheliocytes have been investigated using duodenal glands of cow and sheep as a model system. It has been ascertained, that con A- and LCA-binding sites (mannosglycans) localize exclusively in basal cytoplasm of duodenal gland epitheliocytes, which corresponds to rough endoplasmic reticulum. Manifestation of D-galactose, L-fucose, N-acetyl-D-galactosamine residues within oligosaccharide changes (PNA-, RCA-, WGA- and LAA-reactivity) was detected in the supranuclear region and apical cytoplasm of glandulocytes, that corresponds to Golgi complex, SBA-reactive glycoconjugates were determined only in poorly differentiated cells, forming initial parts of glandular ducts, but were absent in more differentiated cells of superficial and profound acini. The results obtained demonstrate fine prospects that are opened by the use of lectin-peroxidase kits in precise analysis of glycopolymers processing within functionally active and differentiating glandular cells.
Lutsik
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Iatskovskiĭ
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D000818:Animals; D001704:Biopolymers; D002417:Cattle; D004386:Duodenum / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D006031:Glycosylation; D006651:Histocytochemistry; D006801:Humans; D007231:Infant, Newborn; D011975:Receptors, Mitogen / Q000032:analysis*; D012756:Sheep
D001704:Biopolymers; D011975:Receptors, Mitogen
Ispol'zovanie metoda lektinogistokhimii dlia kharakteristiki protsessov glikozilirovaniia biopolimerov v kletkakh duodenal'nykh zhelez.