Identification of fructose 3-phosphate in the lens of diabetic rats.
Fructose 3-phosphate, a novel monosaccharide phosphate, has been identified in the lens of diabetic rats. This compound, which is not present in normal lenses, is a protein glycosylating agent and enzyme inactivator. In addition, because of its structural features, this metabolite is relatively labile and undergoes hydrolysis to yield inorganic phosphate and the potent glycosylating agent, 3-deoxyglucosone. The increase in the concentration of fructose 3-phosphate in the lens of diabetic rats suggests that it and its hydrolysis product, 3-deoxyglucosone, may be responsible in part for the development of some diabetic complications in the lens.
Szwergold
B S
BS
Department of Nuclear Magnetic Resonance and Medical Spectroscopy, Fox Chase Cancer Center, Philadelphia, PA 19111.
Kappler
F
F
Brown
T R
TR
D000818:Animals; D003432:Cross-Linking Reagents; D003847:Deoxyglucose / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005634:Fructose-Bisphosphate Aldolase / Q000037:antagonists & inhibitors; D005636:Fructosephosphates / Q000032:analysis* / Q000378:metabolism / Q000494:pharmacology; D006031:Glycosylation; D006868:Hydrolysis; D007770:L-Lactate Dehydrogenase / Q000037:antagonists & inhibitors; D007908:Lens, Crystalline / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D051381:Rats
D003432:Cross-Linking Reagents; D005636:Fructosephosphates; C062519:fructose 3-phosphate; D003847:Deoxyglucose; D007770:L-Lactate Dehydrogenase; D005634:Fructose-Bisphosphate Aldolase; C016350:3-deoxyglucosone