title: Using polymerized C9 to produce a monoclonal antibody against a neoantigen of the human terminal complement complex.
abstract: The terminal complement complex (TCC), consisting of C5b, C6, C7, C8, and C9, contains neoantigens that are absent from the individual native components. Neoantigens are present both in the membrane-bound (MAC) and the fluid-phase (SC5b-9) complex. The present study describes production of monoclonal antibodies (MoAbs) against neoantigens of both forms of the TCC using human C9 polymerized in Zn2+ as an immunogen. One of ten MoAbs obtained, MoAb 1B4, reacted with the tubular C9 polymer, but not with either the native or sodium dodecyl sulfate-denatured monomeric C9, as shown by enzyme-linked immunosorbent assay and Western blotting. Moreover, MoAb 1B4 cross-reacted both with MAC and SC5b-9. This suggests MoAb 1B4 recognized a neoantigen in the moiety of C9 polymer in the TCC. MoAb 1B4 will be of value for definitive identification of MAC and for quantitation of SC5b-9 in plasma and urine.
authors.affiliation: Department of Pediatrics, School of Medicine, Hokkaido University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kusunoki
authors.forename: Y
authors.initials: Y
authors.lastname: Takekoshi
authors.forename: S
authors.initials: S
authors.lastname: Nagasawa
mesh_terms: D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000941:Antigens / Q000276:immunology*; D003186:Complement C9 / Q000276:immunology*; D015938:Complement Membrane Attack Complex / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D012967:Sodium Dodecyl Sulfate; D015032:Zinc / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D003186:Complement C9; D015938:Complement Membrane Attack Complex; D012967:Sodium Dodecyl Sulfate; D015032:Zinc