title: Confirmation of an ultrasound-induced mutation in two in-vitro mammalian cell lines.
abstract: In-vitro V79 and L5178Y cells were exposed in a rotating test tube to continuous-wave (CW) 1 MHz 35 W/cm2 ultrasound (0-4 or 0-3 min, respectively) and subsequently assayed for mutation as evidenced by resistance to 6-thioguanine (6-TG). There was a modest but statistically significant increase in mutation frequency in both cell types with increase in ultrasound exposure duration. X-ray exposures (3-9 Gy, a "positive control") yielded a large increase in 6-TG resistance. The data support an earlier report by Kaufman (1985) of an ultrasound-induced increase in mutation to 6-TG resistance in in-vitro mammalian cells.
authors.affiliation: Biology Department, Shiga University of Medical Science, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Doida
authors.forename: M W
authors.initials: MW
authors.lastname: Miller
authors.forename: C
authors.initials: C
authors.lastname: Cox
authors.forename: C C
authors.initials: CC
authors.lastname: Church
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D006224:Cricetinae; D066298:In Vitro Techniques; D007940:Leukemia L5178; D009152:Mutagenicity Tests; D009154:Mutation; D013866:Thioguanine / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014465:Ultrasonics / Q000009:adverse effects*
chemical_list: D013866:Thioguanine