Structure of human low-density lipoprotein subfractions, determined by X-ray small-angle scattering.
The structure of low-density lipoprotein (LDL) particles from three different density ranges (LDL-1: d = 1.006-1.031 g/ml; LDL-3: d = 1.034-1.037 g/ml; LDL-6: d = 1.044-1.063 g/ml) was determined by X-ray small-angle scattering. By using a theoretical particle model, which accounted for the polydispersity of the samples, we were able to obtain fits of the scattering intensity that were inside the noise interval of the measured intensity. The assumption of deviations from radial symmetry is not supported by our data. This implies a spread-out conformation of the apolipoprotein B (apoB) molecule, which appears to be localized in the outer surface shell. A globular structure is not consistent with our data. Furthermore, different models exist concerning the structure of the cholesterol ester core below the phase transition temperature. The electron density data suggest an arrangement in which the steroid moieties are localized at average radii of 3.2 and 6.4 nm. Model calculations show that packing problems can only be avoided if approximately half of the acyl chains of each shell are pointing towards the center of the particle, the other half towards the surface. This arrangement of the acyl chains has never been proposed before. The LDL particles of different density classes differ mainly with respect to the size of the core but also with respect to the width of the surface shells. Model calculations show that the size of different LDL particles can be accurately predicted from the compositional data.
Baumstark
M W
MW
Institut für Biophysik und Strahlenbiologie der Universität Freiburg im Briesgau, F.R.G.
Kreutz
W
W
Berg
A
A
Frey
I
I
Keul
J
J
D001055:Apolipoproteins B / Q000032:analysis; D005583:Fourier Analysis; D006801:Humans; D008055:Lipids / Q000032:analysis; D008077:Lipoproteins, LDL / Q000032:analysis / Q000648:ultrastructure*; D046911:Macromolecular Substances; D008297:Male; D015394:Molecular Structure; D008970:Molecular Weight; D011487:Protein Conformation; D012542:Scattering, Radiation; D013696:Temperature; D014965:X-Rays
D001055:Apolipoproteins B; D008055:Lipids; D008077:Lipoproteins, LDL; D046911:Macromolecular Substances