title: Genetic mapping and biochemical analysis of mutants in the maltose regulatory gene of the MAL1 locus of Saccharomyces cerevisiae.
abstract: The MAL1 locus of Saccharomyces cerevisiae comprises three genes necessary for maltose utilization: a regulatory (MALR), a maltose transport (MALT) and a maltase gene (MALS). A fine structure genetic map of the MAL1R gene was constructed and the order of mutations was confirmed by plasmid-mediated chromosomal recombination. The mutations cluster non-randomly within the 5' half of the gene, where the putative DNA binding domain of the encoded protein is located. Only mutations mal1R-22 and MAL1R-72 map in the 3' terminal half of the gene; these mutations cause a different pattern of transcriptional regulation of plasmid-borne MAL6T genes. Experiments supporting a direct involvement of the MALR-encoded protein in carbon catabolite repression of MAL gene expression are reported.
authors.affiliation: Department of Biology, Rutgers University, Camden, NJ 08102.
authors.forename: M J
authors.initials: MJ
authors.lastname: Goldenthal
authors.forename: M
authors.initials: M
authors.lastname: Vanoni
mesh_terms: D015152:Blotting, Northern; D002874:Chromosome Mapping; D004274:DNA, Recombinant; D015966:Gene Expression Regulation, Fungal; D005809:Genes, Regulator; D008320:Maltose / Q000235:genetics* / Q000378:metabolism; D008540:Meiosis; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D004274:DNA, Recombinant; D008320:Maltose