Evaluation of LAK-mediated tumor cell killing in a plasma clot clonogenic assay.
An assay based on the inhibition of the cloning capacity in a plasma clot semisolid medium assay has been used to test the sensitivity of the Raji cell line to lymphokine-activated killer (LAK) cells. This method overcomes some limitations intrinsic to the widely employed 51Cr release assay and always shows a higher degree of sensitivity. No inhibition of colony growth was found when the effector cells were plated without prior pre-incubation with interleukin 2 or with the addition of the medium derived from the LAK cells. Though more time-consuming than the classic 51Cr release assay, this technique does not require radioactive material. This test may be suitable for a more precise evaluation of LAK activity and for the study of the mechanisms involved in cell killing.
Dinota
A
A
Divisione di Ematologia, Ospedale S. Carlo, Bologna, Italy.
Tazzari
P L
PL
Bontadini
A
A
Raspadori
D
D
Tassi
C
C
Zucchini
L
L
Pizzuti
M
M
Ricciuti
F
F
Gobbi
M
M
D002051:Burkitt Lymphoma / Q000473:pathology; D003601:Cytotoxicity Tests, Immunologic; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D014407:Tumor Cells, Cultured; D014410:Tumor Stem Cell Assay
D007376:Interleukin-2