Immunolocalisation of an anti-EMA monoclonal antibody to a renal carcinoma xenograft.
The monoclonal antibody 77-1 originally raised to a membrane extract of human bladder cancer reacted with a human renal carcinoma xenograft XK1 on immunoperoxidase staining. This suggested a potential role for its immunolocalisation to renal carcinoma, and prompted a localisation study of the I-125 and In-111 labelled 77-1 to the xenograft. The uptake of the radiolabelled antibody by the xenograft was measured as a tumour to blood ratio taken at 24 hour intervals for up to 72 hours. Four animals were used for each time point and the mean tumour to blood ratio obtained. A monoclonal antibody 48-1, non reactive with XK1, was similarly radiolabelled and administered to another group of animals bearing the xenografts to act as a control. Statistical analysis using the unpaired Student't' test showed that the uptake of the 1-125 and In-111 labelled 77-1 by the xenografts was significantly higher than the non-specific antibody 48-1 at all time points (p less than 0.002-0.018), demonstrating that a specific localisation to the xenograft XK1 by the radiolabelled 77-1 had occurred.
Yiu
C Y
CY
Department of Surgery, University College London, England.
Baker
L A
LA
Boulos
P B
PB
D000818:Animals; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000276:immunology; D001345:Autoradiography; D002292:Carcinoma, Renal Cell / Q000000981:diagnostic imaging / Q000276:immunology* / Q000473:pathology; D002460:Cell Line; D006801:Humans; D007124:Immunoenzyme Techniques; D007205:Indium Radioisotopes; D007457:Iodine Radioisotopes; D007680:Kidney Neoplasms / Q000000981:diagnostic imaging / Q000276:immunology* / Q000473:pathology; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008808:Mice, Inbred CBA; D018396:Mucin-1; D011877:Radionuclide Imaging; D013934:Thymectomy; D014183:Transplantation, Heterologous
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D007205:Indium Radioisotopes; D007457:Iodine Radioisotopes; D008562:Membrane Glycoproteins; D018396:Mucin-1