title: A functional evaluation of cryopreserved peripheral nerve autografts.
abstract: This study compared the functional return following the repair of a 2.0-cm defect in the rat sciatic nerve with frozen (with or without treatment with a cryoprotectant, dimethyl sulfoxide [DMSO]) or fresh nerve grafts. In addition, a control group in which the defect was left ungrafted was evaluated. Recovery was assessed by functional studies (sensory testing, gait analysis, joint contractures, and tibialis anterior muscle weight), biochemical analysis (muscle hydroxyproline concentration), and histologic studies (axonal counts and fiber density). Sensory testing and gait analysis were not helpful because of cross-over innervation of the saphenous nerve and lack of recovery of the foot intrinsic muscles, respectively. The hind-limb contractures of the group with fresh nerve grafts (18 degrees +/- 3 degrees) were similar to those with DMSO-cryopreserved grafts (14 degrees +/- 4 degrees). Both of these groups had statistically smaller contractures than the non-DMSO-cryopreserved group (45 degrees +/- 8 degrees) and the gap control group (49 degrees +/- 6 degrees), (p less than 0.01). The tibialis anterior (TA) muscle-weight ratio (MWR) and the muscle-hydroxyproline-concentration ratio (MHPCR) showed a similar pattern, with no significant difference between the fresh (MWR, 0.5785 +/- 0.068 and MHPCR, 1.527 +/- 0.405) and DMSO-cryopreserved (MWR, 0.5675 +/- 0.0989 and MHPCR, 1.660 +/- 0.456) groups; both of these groups showed greater neurologic recovery when compared to the non-DMSO-cryopreserved (MWR, 0.3364 +/- 0.0266 and MHPCR, 3.441 +/- 0.300) and gap control (MWR, 0.2134 +/- 0.0775 and MHPCR, 4.869 +/- 2.351) groups.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Orthopaedics and Rehabilitation, Yale University School of Medicine, New Haven, Connecticut.
authors.forename: P A
authors.initials: PA
authors.lastname: Ruwe
authors.forename: T E
authors.initials: TE
authors.lastname: Trumble
mesh_terms: D000818:Animals; D015925:Cryopreservation; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005684:Gait; D006085:Graft Survival / Q000187:drug effects; D006909:Hydroxyproline / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron; D008866:Microsurgery / Q000379:methods; D009119:Muscle Contraction / Q000502:physiology; D009132:Muscles / Q000294:innervation / Q000378:metabolism / Q000473:pathology; D009413:Nerve Fibers, Myelinated / Q000473:pathology; D009929:Organ Size; D051381:Rats; D011919:Rats, Inbred Strains; D012584:Sciatic Nerve / Q000473:pathology / Q000502:physiology / Q000637:transplantation*; D012677:Sensation; D013977:Tibia; D014021:Tissue Preservation
chemical_list: D006909:Hydroxyproline; D004121:Dimethyl Sulfoxide