[Experimental studies on canine tracheal preservation].
Thirty-five adult mongrel dogs were anesthetized and a five-ring segment of cervical trachea was excised. The tracheal defect was restored by end-to-end anastomosis. The autografts were divided into 4 groups as follows; no preservation (control) group (n = 4), graft preserved in Collins solution (n = 11), graft preserved in phosphate buffered extracellular solution (Ep4) (n = 11), and graft preserved in physiological saline solution (n = 11) for 24 or 48 hours at 4 degrees C. After preservation, laparotomy was performed on the animals and the tracheal autograft was wrapped in the distal portion of the omentum. After 21 days all grafts were excised and examined by macroscopically, microscopically and scanning electron microscopically (SEM). The changes of the epithelia glands, and cilia were evaluated, with each item being rated on a point scale (1-3) according to the degree of regeneration and summed to form a total score. The total score of the Collins group was significantly higher than those of the Ep4 and saline groups. The present data suggest that tracheal autografts can be preserved for up to 48 hours in each of the solutions and that Collins solution is preferable for use in canine tracheal preservation.
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Second Department of Surgery, Okayama University Medical School, Japan.
D000818:Animals; D004285:Dogs; D009926:Organ Preservation / Q000379:methods; D014132:Trachea / Q000637:transplantation / Q000648:ultrastructure; D014182:Transplantation, Autologous