The expression of rat brain synaptic plasma membrane Na(+)-Ca2+ exchange activity in Xenopus oocytes.
Injection of Xenopus oocytes with mRNA isolated from 1-day-old rat brains leads to expression of Na+ gradient-dependent Ca2+ uptake activity. Size fractionation of the mRNA by sucrose density gradient centrifugation reveals that an mRNA fraction enriched in 14-18 S mRNA is responsible for the transport activity. Plasma membrane proteins isolated from oocytes injected with total or 14-18 S enriched fraction of rat brain mRNA contain proteins of about 70-kDa molecular mass recognized by a polyclonal antibody prepared against the purified 70-kDa rat brain Na(+)-Ca2+ exchanger. In control H2O-injected oocytes, no proteins are recognized by the anti-70-kDa antibody.
Furman
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I
Department of Biochemistry, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Rahamimoff
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H
D000818:Animals; D001923:Brain Chemistry; D002118:Calcium / Q000378:metabolism*; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D008845:Microinjections; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009865:Oocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis; D019831:Sodium-Calcium Exchanger; D013570:Synaptic Membranes / Q000737:chemistry*; D014982:Xenopus laevis
D002352:Carrier Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D019831:Sodium-Calcium Exchanger; D002118:Calcium