title: Preliminary experience with the differential pH technique for glucose-6-phosphate dehydrogenase (G6PD) measurement in whole blood: application to an area with high prevalence of thalassaemia and G6PD deficiency.
abstract: A new differential pH technique for glucose-6-phosphate dehydrogenase quantitative determination in whole blood has been evaluated. It is a rapid (90 s/analysis), reproducible (C.V. within-run 3.7%; between-run: 2.8%) and accurate method (in comparison with WHO method: r = 0.970). Reference intervals in non-deficient males were evaluated in 167 non-thalassaemics and in 60 beta-thal heterozygotes. The G6PD activity in beta-thalassaemia carriers is higher than in normals; this is particularly true if the activity is expressed in terms of U/g Hb. The phenotypic distribution measured in females is in agreement with that calculated by the Hardy-Weinberg law based on the incidence of the Gd(-) gene in males.
authors.affiliation: Dipartimento di Scienze e Tecnologie Biomediche, Università, Milano, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Mosca
authors.forename: M
authors.initials: M
authors.lastname: Paderi
authors.forename: A
authors.initials: A
authors.lastname: Sanna
authors.forename: R
authors.initials: R
authors.lastname: Paleari
authors.forename: A
authors.initials: A
authors.lastname: Cao
authors.forename: R
authors.initials: R
authors.lastname: Galanello
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D003937:Diagnosis, Differential; D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D005787:Gene Frequency; D006580:Genetic Carrier Screening; D005954:Glucosephosphate Dehydrogenase / Q000097:blood*; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000175:diagnosis* / Q000201:enzymology / Q000453:epidemiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007558:Italy / Q000453:epidemiology; D008297:Male; D011199:Potentiometry; D011237:Predictive Value of Tests; D013789:Thalassemia / Q000175:diagnosis* / Q000201:enzymology / Q000453:epidemiology
chemical_list: D005954:Glucosephosphate Dehydrogenase