Control of the adipsin gene in adipocyte differentiation. Identification of distinct nuclear factors binding to single- and double-stranded DNA.
The mouse adipsin gene encodes a serine protease with complement factor D activity that is expressed during adipocyte differentiation and is deficient in several animal models of obesity. We have investigated the regulation of adipsin expression by transfecting preadipocytes and adipocytes with plasmids containing the 5'-flanking region of the adipsin gene linked to a reporter gene. Constructions containing a -950 to +35 segment of the adipsin promoter were preferentially expressed in adipose cells. Deletion experiments identified a region from -114 to -38 which contains a large inverted repeat sequence and negatively regulated gene expression in preadipocytes and positively regulated expression in fat cells. Exonuclease III protection and gel retardation assays indicated that this region of duplex DNA had multiple binding sites for nuclear factors, several of which were preadipose specific. In addition, we also identified two distinct factors that bound symmetrically and sequence specifically to the inverted repeat sequences only when they were in single-stranded form; one of these factors was induced during adipocyte differentiation. These results suggest that the control of the adipsin promoter in differentiation may involve an interplay of multiple regulated DNA-binding proteins, including two that have preferential affinity for single-stranded DNA.
Wilkison
W O
WO
Dana-Farber Cancer Institute, Boston, Massachusetts 02115.
Min
H Y
HY
Claffey
K P
KP
Satterberg
B L
BL
Spiegelman
B M
BM
D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002454:Cell Differentiation; D002460:Cell Line; D011416:Complement Factor D; D004247:DNA / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism*; D005090:Exodeoxyribonucleases; D005786:Gene Expression Regulation; D051379:Mice; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012697:Serine Endopeptidases / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism*; D014162:Transfection
D004277:DNA, Single-Stranded; D014157:Transcription Factors; D004247:DNA; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III; D012697:Serine Endopeptidases; D011416:Complement Factor D; C493491:complement factor D, mouse