Butylated hydroxyanisole increases glutathione-S-transferase activity in hepatocyte culture.
The effect of butylated hydroxyanisole (BHA) on the activity of glutathione-S-transferase (GSH-S-T) in hepatocyte culture was determined. Hepatocytes from control rats and rats injected with 3-methylcholanthrene (3-MC) were isolated and cultured. The cultures were treated with various concentrations of BHA. Treatment of hepatocytes from both control and 3-MC-injected rats with 25 microM BHA for 24 or 48 hours increased GSH-S-T activity significantly. The results suggest that BHA may have a detoxifying effect against 3-MC-induced carcinogenesis, and that hepatocyte culture may be a fast and simple system to identify compounds which may potentially protect against development of cancer through induction of GSH-S-T.
Chen
L H
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Department of Nutrition and Food Science, Lexington, Kentucky 40506-0054.
D000704:Analysis of Variance; D000818:Animals; D002083:Butylated Hydroxyanisole / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005982:Glutathione Transferase / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values
D002083:Butylated Hydroxyanisole; D008748:Methylcholanthrene; D005982:Glutathione Transferase