Inhibition of hepatic lipase activity impairs chylomicron remnant-removal in rats.
[4-14C]Cholesteryl oleyl ether-labeled chylomicron remnants were injected into rats which received a specific goat antibody against rat hepatic lipase or a control serum. Chylomicron remnant cholesterol ether disappeared from circulation with a significantly higher half-life (2-fold) in antibody-treated rats than in controls (P less than 0.001). Recovered radioactivity in the liver was 2-fold lower in antibody-treated rats (22.8% (n = 6) vs. 45% (n = 4) P less than 0.01). These results clearly show that hepatic lipase may strongly promote chylomicron remnant cholesterol ether uptake by the liver.
Sultan
F
F
INSERM U 177, Institut Biomédical des Cordeliers, Paris, France.
Lagrange
D
D
Jansen
H
H
Griglio
S
S
D000818:Animals; D002914:Chylomicrons / Q000378:metabolism*; D008049:Lipase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D002914:Chylomicrons; D008049:Lipase