Strain differences of I-compounds in relation to organ sites of spontaneous tumorigenesis and non-neoplastic renal disease in mice.
Possible associations between the patterns and levels of I-compounds (putative endogenous, age-dependent, adduct-like DNA derivatives) and the incidence of spontaneous tumors and renal diseases in mice were investigated by 32P-postlabeling assay. Liver, lung and kidney DNAs of 8-10 month old inbred male mice of strains C57BL, C3H, CBA, A and RFM were examined. It was found that liver DNA of the C3H mouse, known to develop a high incidence of spontaneous hepatomas, and lung DNA of the A mouse, known to have a high incidence of spontaneous lung tumors, contained smaller numbers and significantly lower total levels of I-compounds as compared with the resistant C57BL mouse. Kidney DNA of C57BL, C3H and A mice presented significantly higher levels of I-compounds compared with those of CBA and RFM mice, both of which are known for exceptionally high incidences of spontaneous renal diseases. Total kidney I-compound levels in CBA and RFM mice were only 30-40% of those found in C3H, A and C57BL. Reduced I-compound levels observed in the three organs thus tended to be associated with spontaneous tumorigenesis and degenerative disease in susceptible strains, suggesting that I-compounds may be important for normal gene transcription and DNA replication. On the other hand, some of these DNA derivatives may, as adduct-like premutagenic lesions, contribute to spontaneous tumorigenesis. This was suggested by the finding that CBA mice which exhibit moderate spontaneous hepatoma incidence, had higher levels of certain I-compounds in their liver DNA although the total levels were not significantly different compared with those in C57BL mice. The observations of the present study suggest that (i) the patterns and levels of I-compounds are genetically determined and (ii) lack or excess of these DNA modifications may result in adverse health effects; however, this may not always be the case, for example, if modulating factors exist which compensate for I-compound deficiency.
Li
D H
DH
Department of Pharmacology, Baylor College of Medicine, Houston, TX 77030.
Randerath
K
K
D000367:Age Factors; D000818:Animals; D004247:DNA / Q000378:metabolism*; D007668:Kidney / Q000032:analysis; D007674:Kidney Diseases / Q000209:etiology / Q000662:veterinary*; D008099:Liver / Q000032:analysis; D008168:Lung / Q000032:analysis; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009369:Neoplasms / Q000209:etiology / Q000662:veterinary*; D012376:Rodent Diseases / Q000209:etiology*; D013045:Species Specificity
D004247:DNA