[Construction of a complete "library" of mutants at region -35 of a model prokaryotic promotor].
A novel approach to study the variability of consensus sites of regulatory regions of DNA is proposed. The overall strategy includes chemical synthesis of representative series of all possible DNA structures under investigation, cloning and screening according to their function. The chemical-enzymatic synthesis of a complete library of 40-bp DNA duplexes, corresponding to the model prokaryotic promoter and differing in 6-membered segments at -35 region, is described.
Drutsa
V L
VL
Krivonos
A V
AV
Koroleva
O N
ON
Shabarova
Z A
ZA
D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D015723:Gene Library; D008969:Molecular Sequence Data; D009154:Mutation; D009692:Nucleic Acid Heteroduplexes; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid
D009692:Nucleic Acid Heteroduplexes; D004247:DNA
Poluchenie polnoĭ "biblioteki" mutantov oblasti -35 model'nogo prokaryioticheskogo promotora.