[Photoperiodic variations in plasma concentration of testosterone in the rabbit].
The photoperiodic variations of testosterone plasmatic levels in male rabbits have been studied. The animals were subjected to the influence of three circadian rhythms: 12/12, 14/10 and 10/14 h of light/darkness. The hormone assay was carried out using an enzymeimmunoassay method (Competitive ELISA). Blood samples were of two different types: seriated and non-seriated, to find if these might be any differences in the results. It is concluded that testosterone plasmatic levels are within physiologic values (0.3-10.0 ng/ml), although differences depending on the light/darkness cycle and on the type of blood extraction are remarkable; for this reason the selection of the circadian rhythm will depend on the experimentation that is to be carried out.
Silván
G
G
Departamento de Fisiología animal, Facultad de Veterinaria, U.C.M., Madrid, España.
Illera
J C
JC
Martín
J
J
Manjón
R
R
Illera
M
M
D000818:Animals; D002940:Circadian Rhythm / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D008297:Male; D011817:Rabbits; D013739:Testosterone / Q000097:blood*
D013739:Testosterone
Variaciones fotoperiódicas de las concentraciones plasmáticas de testosterona en conejo.