title: In vivo thrombus formation induced by complement activation on polymer surfaces.
abstract: To clarify involvement of complement activation in thrombus formation on polymer surfaces, in vitro complement activation was evaluated for polyethylene (PE) tubes radiation-graft copolymerized with acrylamide (AAm), acrylic acid (AC), 2-hydroxyethyl methacrylate (HEMA), N-vinylpyrrolidone (NVP), and vinyl alcohol (VOH), and compared to their in vivo antithrombogenicity and cell adherence in canine peripheral veins. The complement-activating surfaces (NVP and VOH) cause preferential adhesion of leukocytes and were more thrombogenic than the low complement-activating surfaces (AAm, PE, and HEMA). Infusion of naja haje cobra venom factor depressed leukocyte adhesion, followed by a marked decrease in thrombogenesis, for the strong classical-pathway-activating surface (NVP). Although estimation of in vitro activation for AC was inconclusive because of a large effect of adsorption, AC behaved like VOH in vivo. These results suggest that C5a(des Arg) mediated activation of leukocytes may play a role in thrombus formation by complement activation on polymer surfaces.
authors.affiliation: Government Industrial Research Institute, Osaka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
authors.forename: H
authors.initials: H
authors.lastname: Fukumura
authors.forename: N
authors.initials: N
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D003167:Complement Activation / Q000187:drug effects*; D004285:Dogs; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D010973:Platelet Adhesiveness; D011095:Polyethylenes / Q000737:chemistry / Q000494:pharmacology*; D013499:Surface Properties; D013927:Thrombosis / Q000209:etiology*
chemical_list: D011095:Polyethylenes