title: Alterations in the major heterotrophic bacterial populations isolated from a still bottled mineral water.
abstract: The heterotrophic bacterial population of a bottled mineral water stored in returnable glass bottles and in polyvinyl chloride (PVC) bottles at room temperature was studied over 9-12 months. The plate counts in R2A medium incubated at 22 degrees and 37 degrees C were low initially, increasing to 10(4)-10(5) cfu/ml within a few days of bottling. The number of bacteria recovered at 22 degrees C from PVC bottles was fairly constant during the storage period, but the population isolated at 37 degrees C decreased markedly after storage for 1 year. The major components of the population were Pseudomonas strains, one of which was identified as Pseudomonas vesicularis. Major changes took place during storage; two groups of bacteria (B and C) were dominant initially, but during the latter period of storage other groups (F, G and H) increased in number.
authors.affiliation: Departamento de Zoologia, Universidade da Coimbra, Portugal.
authors.forename: P V
authors.initials: PV
authors.lastname: Morais
authors.forename: M S
authors.initials: MS
authors.lastname: da Costa
mesh_terms: D001419:Bacteria / Q000254:growth & development*; D015169:Colony Count, Microbial; D005516:Food Microbiology; D005519:Food Preservation; D005898:Glass; D008900:Mineral Waters; D010641:Phenotype; D011143:Polyvinyl Chloride; D014871:Water Microbiology
chemical_list: D008900:Mineral Waters; D011143:Polyvinyl Chloride