Effect of dietary lipids on hepatic and intestinal monooxygenases in mice.
The effect of dietary lipids on hepatic and intestinal monooxygenases was studied by feeding C57BL/6N mice (for 2 wks) diets containing 5% and 23.5% (wt/wt) olive oil or corn oil. At the end of the feeding period, we measured arylhydrocarbon hydroxylase (AHH) activity in S9 preparations from liver, small intestine, and colon; and, using the same S9 preparations from the liver, we observed the activation of the following three dietary promutagens: 2-amino-3-methylimidazo(4,5-f)quinoline, 2-amino-3,8-dimethylimidazo(4,5-f) quinoxaline, and 2-amino-6-methyldipyrido(1,2-a:3',2'-d)imidazole. The results showed that high-fat diets increased hepatic AHH activity both in corn oil and olive oil diets compared with the low-fat diets; also, a 5% corn oil diet had significantly higher AHH activity compared with the 5% olive oil diet. AHH activity was, respectively, 48.6 +/- 5.1 and 79.5 +/- 11.4 pmol 3OH-benzo[a]pyrene formed/mg/min in the 5% and 23.5% olive oil diets and 66.1 +/- 5.1 and 83.9 +/- 12.2 in the 5% and 23.5% corn oil diets; values are means +/- SE, n = 16. The results also showed a significant increase in the ability of hepatic S9 fractions from animals on high-fat diets to activate promutagens in the Salmonella/plate test. On the contrary, AHH activity in the small intestine and colon was not affected by the fat content of the diet.
Caderni
G
G
Department of Pharmacology, University of Florence, Italy.
Bianchini
F
F
Dolara
P
P
Lodovici
M
M
Quattrucci
E
E
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000032:analysis*; D003106:Colon / Q000201:enzymology; D004041:Dietary Fats / Q000494:pharmacology*; D005260:Female; D007422:Intestines / Q000201:enzymology*; D008055:Lipids / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology*; D051379:Mice; D008810:Mice, Inbred C57BL
D004041:Dietary Fats; D008055:Lipids; D001189:Aryl Hydrocarbon Hydroxylases