[Qualitative and quantitative studies of synovial lesions in early rheumatoid arthritis demonstrated by immunocytochemistry with monoclonal antibodies].
We investigate the early stage of synovial lesions in 12 cases of rheumatoid arthritis (RA), which were diagnosed according to the criteria refined by America Rheumatism Association in 1987. The purpose of this study was to follow the inflammatory process from beginning to well known typical inflammatory features which are composed of a lot of lymphocytes and proliferated capillaries and the synovial cells. The characteristics of this study were that 1) the arthroscopy was applied for observation and getting synovial tissues, 2) 7 kinds of monoclonal antibodies were used for biopsied synovial tissues in immunohistochemistry study, further 3) qualitative and quantitative methods were adopted for identifying the infiltrating cells. These methods have developed recently in the fields of orthopaedics and immuno-histopathology, respectively. In very early stage, first, HLA/DR positive cells with large elongated cytoplasm accompanied but a few processes were found in pericapillary regions. These were neither macrophages nor lymphocytes. Following them T lymphocytes appeared and B lymphocytes appeared to aggregate in later. Quantitatively the number of HLA/DR positive cells including macrophages, B lymphocytes and a part of T lymphocytes and of Interstitial cells, increase and occupied the fairly large number of ratio in the cells of synovial tissues in RA.
Sawai
T
T
First Department of Pathology, Tohoku University School of Medicine, Senday.
Ohyama
A
A
Murakami
K
K
Rikimaru
A
A
Hattori
A
A
Kyogoku
M
M
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D001172:Arthritis, Rheumatoid / Q000175:diagnosis* / Q000473:pathology; D001182:Arthroscopy; D002452:Cell Count; D005260:Female; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D013583:Synovial Membrane / Q000166:cytology* / Q000473:pathology
D000911:Antibodies, Monoclonal; D006684:HLA-DR Antigens