Impaired generation of prostaglandins from isolated gastric surface epithelial cells in portal hypertensive rats.
We studied generation of prostaglandins E2 and 6-keto F1a by surface epithelial cell isolated from the gastric mucosa of portal hypertensive and sham-operated rats. Oxygenated cell suspensions containing 80 +/- 3% of surface epithelial cells were incubated for 30 min at 37 degrees C and the concentration of prostaglandin E2 and 6-keto-prostaglandin F1a in medium was measured by radioimmunoassay. Viability of the cells was assessed with Fast green exclusion at baseline and after 30-min and 60-min incubation. Within 30 minutes the surface epithelial cells obtained from portal hypertensive rats generated 22.0 +/- 1.6 (mean +/- SE) pg prostaglandin E2 and 40.7 +/- 4.7 pg 6-keto prostaglandin F1a, per 10(6) cells. These were significantly less than prostaglandin generation by cells obtained from sham-operated rats. The viability of the surface epithelial cells from portal hypertensive rats was also significantly reduced compared with sham-operated rats after 60 minute incubation. Reduced ability of the surface epithelial cells to generate prostaglandins may be one mechanism for increased susceptibility of portal hypertensive gastric mucosa to injury by noxious agents.
Arakawa
T
T
Veterans Administration Medical Center, Long Beach, California 90822.
Tarnawski
A
A
Mach
T
T
Kobayashi
K
K
Sarfeh
I J
IJ
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis*; D000818:Animals; D002470:Cell Survival; D015232:Dinoprostone / Q000096:biosynthesis*; D004848:Epithelium / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism* / Q000473:pathology; D006975:Hypertension, Portal / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D015121:6-Ketoprostaglandin F1 alpha; D015232:Dinoprostone