title: Capillary gel electrophoresis for DNA sequencing. Laser-induced fluorescence detection with the sheath flow cuvette.
abstract: Capillary polyacrylamide gel electrophoresis separation of dideoxycytidine chain-terminated DNA fragments is reported. A post-column laser-induced fluorescence detector based on the sheath flow cuvette was used to minimize background signals due to light scatter from the gel and capillary. A preliminary mass detection limit of 10(-20) mol of fluorescein-labeled DNA fragments was obtained. The system was used to analyze an actual DNA sequencing sample. Theoretical plate counts of 2 x 10(6) were produced. Gel stability limits the performance of the current system.
authors.affiliation: Howard Hughes Medical Institute, University of Utah, Salt Lake City 84132.
authors.forename: H
authors.initials: H
authors.lastname: Swerdlow
authors.forename: S L
authors.initials: SL
authors.lastname: Wu
authors.forename: H
authors.initials: H
authors.lastname: Harke
authors.forename: N J
authors.initials: NJ
authors.lastname: Dovichi
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods*; D005453:Fluorescence; D007834:Lasers
chemical_list: D004247:DNA