[Estrogen receptors in the lower uterine segment].
The presence and quantity of specific cytoplasmatic receptors for E2 were analysed in the smooth muscle cells of the lower uterine segment in 14 patients (mean age 29.5 years, mean parity 2.9) who had a normal pregnancy and delivered at term. Myometrial tissue (greater than 150 mg) was taken from the median zone of the front wall of the lower uterus during cesarian section with a transversal incision on the lower segment at the level of the vescico-uterine fold. Cesarian section was electively chosen by 10 patients before the onset of labour and was used in 4 cases due to complications arising during labour. In order to carry out the receptor assay 100 ml of cytosolic fraction were incubated for 24 h with increasing concentrations of 0.38 microCu/ml to 2.5 microCu/ml of E2 tired in presence or absence of approximately 100-fold higher concentrations of unlabelled E2. Recorded cpm were compared to a standard reference curve. There was no significant difference between the radioactivity levels of the cytosolic fraction measured as total bound or with non-specific bound. Results are fully discussed in the paper.
Iachello
R
R
Istituto di I Clinica Ostetrica e Ginecologica, Università degli Studi di Catania.
Pepe
F
F
Sanfilippo
A
A
Raimondi
M
M
Cernuto
A
A
D000328:Adult; D005260:Female; D006801:Humans; D007743:Labor, Obstetric; D011247:Pregnancy; D011959:Receptors, Estradiol / Q000737:chemistry*; D011960:Receptors, Estrogen / Q000737:chemistry*; D014599:Uterus / Q000737:chemistry*
D011959:Receptors, Estradiol; D011960:Receptors, Estrogen
La recettività estrogenica nel segmento uterino inferiore.