Effect of oxygen free radicals and lipid peroxides on cardiac isoenzymes.
We report here the effect of oxygen free radicals, OFR (superoxide, O2-; hydroxy, OH; t-butylhydroperoxide, H2O2) lipid peroxides (malondialdehyde, MDA), free radical scavengers (superoxide dismutase, catalase, allopurinol) and generator (ferrous chloride) antioxidants (ascorbate, glutathione) spin traps (5,5-dimethyl-1-pyroline-N-oxide, N-t-butyl-L-pheny nitrone) on the cardiac isoenzyme (CK, CK-MB, LDH, LD1) concentrations in the sera of patients with acute myocardial infarction. CK-MB and LD1 were rapidly and completely inactivated by O2- (50 nmol/ml), OH (1 nmol/ml) and MDA (0.6 microM). Butylhydroperoxide (600 microM), and ferrous chloride (200 microM) selectively inhibited CK-MB. The free radical scavengers, antioxidants and spin traps all had minimal effects, and H2O2 had none.
Rao
P S
PS
Division of Cardiothoracic Surgery, Long Island Jewish Medical Center, New Hyde Park, NY 11042.
Rujikarn
N
N
Weinstein
G S
GS
Luber
J M
JM
Tyras
D H
DH
D000975:Antioxidants / Q000494:pharmacology; D003402:Creatine Kinase / Q000037:antagonists & inhibitors; D016166:Free Radical Scavengers; D005609:Free Radicals; D006801:Humans; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000037:antagonists & inhibitors*; D007770:L-Lactate Dehydrogenase / Q000037:antagonists & inhibitors; D008054:Lipid Peroxides / Q000494:pharmacology*; D009203:Myocardial Infarction / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology*; D010100:Oxygen / Q000378:metabolism*
D000975:Antioxidants; D016166:Free Radical Scavengers; D005609:Free Radicals; D007527:Isoenzymes; D008054:Lipid Peroxides; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase; D010100:Oxygen