Glycopeptides in pus of acute pleurisy patients.
Glycopeptides were isolated from tryptic digests of pus from acute pleurisy patients. The hexose, hexosamine, and sialic acid contents rose over the first 2-4 days after admission to the hospital, continued at high levels for 5-8 hospital days, and fell to low levels after 9 hospital days. The course of duration after admission to the hospital was divided into three stages: 1-4 days after admission to the hospital, 5-8 hospital days, and 9-21 hospital days. Materials corresponding to these three stages were then collected for fractionation by DEAE-cellulose column chromatography. Fractionation of glycopeptides by DEAE-cellulose column chromatography yielded three glycopeptide fractions at 0.05 to 0.2 M NaCl. Column chromatography of crude material on DEAE-cellulose showed an increase in the 0.2 M NaCl fraction from the concentrate over a period of 4-8 hospital days due to a large increase in sialic acid-rich glycopeptide fraction.
Masuda
H
H
Third Department of Internal Medicine, University of Occupational and Environmental Health, Fukuoka, Japan.
Ozeki
T
T
Takazono
I
I
Tanaka
Y
Y
D000328:Adult; D000368:Aged; D002848:Chromatography, DEAE-Cellulose; D005260:Female; D006020:Glycopeptides / Q000302:isolation & purification / Q000378:metabolism*; D006595:Hexosamines / Q000032:analysis; D006601:Hexoses / Q000032:analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D010996:Pleural Effusion / Q000378:metabolism; D010998:Pleurisy / Q000378:metabolism*; D012794:Sialic Acids / Q000032:analysis; D013997:Time Factors
D006020:Glycopeptides; D006595:Hexosamines; D006601:Hexoses; D012794:Sialic Acids