Application of high-performance liquid chromatography in establishing an accurate index of blood glucose control.
A chromatographic method utilizing a carboxymethylated poly(vinyl alcohol) resin for a more accurate determination of stable haemoglobin A1c (St-A1c) has been developed. The complete separation between St-A1c, labile HbA1c (L-A1c) and HbF was achieved by gradient elution with sodium chloride in phosphate buffer. This high resolution permits accurate quantitation of St-A1c, even in the presence of high levels of HbF or L-A1c. In 142 subjects with normal fasting plasma glucose and normal response to a 75-g oral glucose tolerance test, the reference interval of St-A1c was 2.80-3.98%.
Hoshino
T
T
Pharmaceutical Institute, School of Medicine, Keio University, Tokyo, Japan.
Takahashi
Y
Y
Suzuki
M
M
D001786:Blood Glucose / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005215:Fasting / Q000378:metabolism; D005951:Glucose Tolerance Test; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans
D001786:Blood Glucose; D006442:Glycated Hemoglobin