title: Hydroxyindole-O-methyltransferase in rat retinal bipolar cells: persistence following photoreceptor destruction.
abstract: The presence of hydroxyindole-O-methyltransferase (HIOMT) activity and localization of HIOMT immunoreactivity was examined in albino rat retinas following photoreceptor destruction. Male Sprague-Dawley rats were exposed to high intensity fluorescent light for 4 consecutive days, then placed on a 14:10 h light:dark cycle for two weeks to allow for phagocytic removal of damaged cells from the retina. Histologic examination revealed almost complete destruction and removal of all photoreceptors. The damaged retinas exhibited an increase in HIOMT activity relative to controls, when expressed as activity per mg of protein. HIOMT activity in the pineal glands was not affected. When control and light damaged retinas were examined for HIOMT localization by immunocytochemistry, the control retinas displayed intense HIOMT immunoreactivity in all photoreceptors, and a somewhat lighter labeling in a population of bipolar cells, whereas the light damaged retinas (lacking photoreceptors) showed intense HIOMT immunoreactivity in bipolar cells. These results suggest that the increase in HIOMT activity following photoreceptor destruction is due to increased synthesis of this enzyme in a population of bipolar cells. These HIOMT-immunoreactive bipolar cells may perhaps respond in a compensatory manner to changing levels of melatonin in the retina.
authors.affiliation: Department of Anatomy, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27103.
authors.forename: A F
authors.initials: AF
authors.lastname: Wiechmann
authors.forename: W K
authors.initials: WK
authors.lastname: O'Steen
mesh_terms: D000121:Acetylserotonin O-Methyltransferase / Q000378:metabolism*; D000818:Animals; D008297:Male; D008780:Methyltransferases / Q000378:metabolism*; D010775:Photic Stimulation; D010786:Photoreceptor Cells / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D012160:Retina / Q000201:enzymology* / Q000473:pathology
chemical_list: D008780:Methyltransferases; D000121:Acetylserotonin O-Methyltransferase