Purification of the glycoprotein glucose oxidase from Penicillium amagasakiense by high-performance liquid chromatography.
Fast protein liquid chromatography (FPLC) in combination with ion-exchange chromatography on a Mono Q column was used to purify glucose oxidase from Penicillium amagasakiense to homogeneity. Purification was performed with a mixed pH and salt gradient, with 20 mM phosphate buffer (pH 8.5) as starting buffer (A) and 50 mM acetate buffer (pH 3.6) with 0.1 M NaCl as elution buffer (B). Elution conditions were optimized to permit the simultaneous purification and separation of the glucose oxidase isoforms. Three peaks, each consisting of 1-2 isoforms and exhibiting a homogeneous titration curve profile, were resolved with a very flat linear gradient of 5.0-5.1% B in 40 ml. Three more peaks, each consisting of several isoforms, were eluted at 10%, 30% and 100% B. Optimization of the elution conditions and separation of the glucose oxidase isoforms was only possible because of the rapidity of each purification step and the high resolution provided by FPLC and Mono Q.
Kalisz
H M
HM
Department of Enzyme Technology, GBF-Gesellschaft für Biotechnologische Forschung, Braunschweig, F.R.G.
Hendle
J
J
Schmid
R D
RD
D002021:Buffers; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005949:Glucose Oxidase / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D010407:Penicillium / Q000201:enzymology*
D002021:Buffers; D005949:Glucose Oxidase