Mitochondrial aspartate aminotransferase determined by "Fast Protein Liquid Chromatography".
We describe an improved separation of the isoenzymes of aspartate aminotransferase (EC 2.6.1.1), based on ion-exchange chromatography. Involving the "Fast Protein Liquid Chromatography" system (Pharmacia) with a MonoQ column, this rapid, reproducible method for quantifying the mitochondrial enzyme shows good resolution and sensitivity, and results correlate well with those by an established immunochemical method.
Grøstad
M
M
Institute of Medical Biology, University of Tromsø, Norway.
Rej
R
R
Huseby
N E
NE
D001219:Aspartate Aminotransferases / Q000097:blood*; D002852:Chromatography, Ion Exchange / Q000379:methods; D006801:Humans; D007120:Immunochemistry; D008929:Mitochondria, Heart / Q000201:enzymology*
D001219:Aspartate Aminotransferases