Non-promoting diterpene esters can induce Epstein-Barr virus early antigen expression in the Raji cell line.
A range of diterpene ester ligands with selective biological activity (e.g., irritant but not tumour promoting) were tested for their ability to induce Epstein-Barr virus (EBV) early antigen expression in the lymphoblastoid Raji cell line. All C12/C13 substituted compounds were found to be capable of inducing some antigen expression at nM-microM levels, including desacetyl-alpha-sapinine, a compound largely devoid of biological activity. The non-promoting, fluorescent compound, sapintoxin A, was virtually equipotent with promoting compounds. It was concluded that, although the assay has relevance to the specific condition of chronic diterpene ester exposure occurring in conjunction with high EBV infection rates, there was relatively poor correlation with mouse skin tumour promoting potential.
Evans
A T
AT
Department of Pharmacognosy, School of Pharmacy, London, U.K.
Brooks
G
G
Evans
F J
FJ
D000956:Antigens, Viral / Q000096:biosynthesis*; D002273:Carcinogens / Q000494:pharmacology; D002460:Cell Line; D004224:Diterpenes / Q000494:pharmacology*; D006801:Humans; D010703:Phorbol Esters / Q000494:pharmacology*; D013329:Structure-Activity Relationship
D000956:Antigens, Viral; D002273:Carcinogens; D004224:Diterpenes; C033305:Epstein-Barr virus early antigen; D010703:Phorbol Esters; C032016:sapintoxin A; C024353:resiniferatoxin