1,2-Diarylethylenediamines as pre-column fluorescence derivatization reagents in high-performance liquid chromatographic determination of catecholamines in urine and plasma.
Meso- and dl-1,2-diarylethylenediamines (14 species) were evaluated for pre-column fluorescence derivatization reagents in the high-performance liquid chromatographic determination of catecholamines (norepinephrine, epinephrine and dopamine) in human urine and plasma. Of the compounds, meso-1,2-bis(4-methoxyphenyl)ethylenediamine was most preferable for all the catecholamines in terms of sensitivity and selectivity. The detection limit for each catecholamine is approximately 0.5 fmol in a 50-microliters injection volume.
Umegae
Y
Y
Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Nohta
H
H
Lee
M
M
Ohkura
Y
Y
D002395:Catecholamines / Q000097:blood / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D005029:Ethylenediamines; D005456:Fluorescent Dyes; D006801:Humans
D002395:Catecholamines; D005029:Ethylenediamines; D005456:Fluorescent Dyes