title: Cell type-specific expression of transforming growth factor-beta 1 in the mouse uterus during the periimplantation period.
abstract: Immunohistochemistry and in situ and Northern blot hybridization were employed to determine temporal and spatial expression of transforming growth factor-beta 1 (TGF beta 1) in the mouse uterus during the periimplantation period. The polyclonal antisera anti-LC-(1-30) and anti-CC-(1-30), raised against two different preparations of a peptide corresponding to the amino-terminal 30 amino acids of TGF beta 1, were used for histochemical analyses because of their distinct staining patterns. Anti-LC shows intracellular staining, while staining by anti-CC is primarily extracellular. The colocalization of intracellular staining by anti-LC with in situ hybridization of TGF beta 1 mRNA in the luminal and glandular epithelia on days 1-4 of pregnancy (day 1 = vaginal plug) indicates that the epithelial cells are the primary sites of TGF beta 1 synthesis during the preimplantation period. On the other hand, staining of the extracellular matrix of the stroma by anti-CC during this period suggests an active accumulation of TGF-beta 1 that is synthesized in and secreted from the epithelia. While intracellular staining and accumulation of TGF-beta 1 mRNA in the epithelia were clearly evident on days 1-4, the extracellular staining showed temporal fluctuations. The clear extracellular staining of the stroma that was observed on day 1 was absent on day 2; moderate staining was again visualized in the stroma on day 3 and was markedly increased on day 4.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Obstetrics-Gynecology, University of Kansas Medical Center, Kansas City 66103.
authors.forename: H
authors.initials: H
authors.lastname: Tamada
authors.forename: M T
authors.initials: MT
authors.lastname: McMaster
authors.forename: K C
authors.initials: KC
authors.lastname: Flanders
authors.forename: G K
authors.initials: GK
authors.lastname: Andrews
authors.forename: S K
authors.initials: SK
authors.lastname: Dey
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D047108:Embryonic Development; D005260:Female; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D016212:Transforming Growth Factor beta / Q000235:genetics*; D014599:Uterus / Q000737:chemistry* / Q000166:cytology
chemical_list: D012333:RNA, Messenger; D016212:Transforming Growth Factor beta