title: Skeletal keratan sulfate chain molecular weight calibration by high-performance gel-permeation chromatography.
abstract: A method has been developed for the molecular sizing of skeletal keratan sulfate chains using an HPLC gel-permeation chromatography system. Keratan sulfate chains and keratanase-derived oligosaccharides were prepared from the nucleus pulposus of bovine intervertebral disc (6-year-old animals). A Bio-Gel TSK 30 XL column eluted in 0.2 M NaCl and at 30 degrees C was calibrated with keratan sulfate oligosaccharides of known size as well as 3H-end-labeled keratan sulfate chains to yield the relationship.
authors.affiliation: Division of Biological Sciences, Lancaster University, Bailrigg, United Kingdom.
authors.forename: J M
authors.initials: JM
authors.lastname: Dickenson
authors.forename: H G
authors.initials: HG
authors.lastname: Morris
authors.forename: I A
authors.initials: IA
authors.lastname: Nieduszynski
authors.forename: T N
authors.initials: TN
authors.lastname: Huckerby
mesh_terms: D000818:Animals; D002138:Calibration; D002417:Cattle; D002467:Cell Nucleus / Q000737:chemistry*; D011508:Chondroitin Sulfate Proteoglycans / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D007632:Keratan Sulfate / Q000737:chemistry*; D000071234:Lumican; D008970:Molecular Weight; D009844:Oligosaccharides / Q000737:chemistry; D013131:Spine / Q000737:chemistry; D014316:Tritium
chemical_list: D011508:Chondroitin Sulfate Proteoglycans; D000071234:Lumican; D009844:Oligosaccharides; D014316:Tritium; D007632:Keratan Sulfate