Isolation of an outer membrane protein complex from Borrelia burgdorferi by n-butanol extraction and high-performance ion-exchange chromatography.
Borrelia burgdorferi, the causative agent of Lyme disease, expresses two major membrane proteins, designated outer surface proteins A and B, which are of antigenic relevance, especially in the chronic phase of Lyme disease. Both proteins exhibit strain-related molecular weight variation. A method is described for obtaining these proteins from the bacterial membrane, without the use of detergents, by a combination of n-butanol extraction and cation-exchange chromatography on a Mono S fast protein liquid chromatographic column. This method yields up to five times larger amounts of the proteins in aqueous solution than previously described protocols, which applied ionic or non-ionic detergents. A comparison of extracts obtained by this method from different Borrelia burgdorferi strains is reported.
Gondolf
K B
KB
Department of Immunology, Institute for Medical Microbiology, Freiburg, F.R.G.
Batsford
S R
SR
Vogt
A
A
D020001:1-Butanol; D001425:Bacterial Outer Membrane Proteins / Q000302:isolation & purification*; D015748:Borrelia burgdorferi Group / Q000032:analysis*; D000440:Butanols; D002462:Cell Membrane / Q000737:chemistry*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight
D001425:Bacterial Outer Membrane Proteins; D000440:Butanols; D020001:1-Butanol