title: Detection of vasoactive intestinal peptide and localization of its mRNA within granulomas of murine schistosomiasis.
abstract: Schistosomiasis mansoni is a parasitic disease resulting in the deposition of ova predominantly in the liver and intestines. These ova secrete antigens which induce host sensitization and evoke focal granulomas. The granulomas are intricate delayed-hypersensitivity reactions governed by numerous cellular and humoral interactions. They displace or destroy normal tissue. Vasoactive intestinal peptide (VIP) is one of several neuropeptides which exert a broad range of biologic actions that may include modulation of immune responses. In this study, VIP was sought within liver granulomas isolated from Schistosoma mansoni-infected, CBA/J mice. Granuloma extracts contained appreciable amounts of immunoreactive VIP as detected by radioimmunoassay. Immunoreactive VIP was shown, by each of two chromatographic methods, to elute as a single peak coinciding with that of synthetic VIP. In situ hybridization was performed with an oligonucleotide probe complementary to a portion of the nucleotide sequence encoding VIP on preproVIP mRNA (antisense probe). Radiolabeled VIP probe adhered exclusively to granuloma eosinophils and to eosinophils within a peritonitis induced in normal mice by proteose peptone. Hybridization of radiolabeled VIP probe in the presence of unlabeled probe substantially attenuated binding. A sense probe failed to bind. These data suggest that the granulomas contain authentic VIP and that eosinophils express the gene for this molecule.
authors.affiliation: Department of Internal Medicine, University of Iowa, Iowa City 52242.
authors.forename: J V
authors.initials: JV
authors.lastname: Weinstock
authors.forename: A M
authors.initials: AM
authors.lastname: Blum
mesh_terms: D000818:Animals; D005260:Female; D015870:Gene Expression; D006099:Granuloma / Q000378:metabolism*; D051379:Mice; D008808:Mice, Inbred CBA; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis*; D012552:Schistosomiasis / Q000378:metabolism*; D014660:Vasoactive Intestinal Peptide / Q000032:analysis* / Q000235:genetics / Q000276:immunology
chemical_list: D012333:RNA, Messenger; D014660:Vasoactive Intestinal Peptide