title: Effect of forskolin on synthesis of xyloside-initiated glycosaminoglycans in embryonic chick chondrocytes.
abstract: The synthesis of sulfated glycosaminoglycan (GAG) chains has been studied in the presence of various concentrations of the artificial acceptor 4-methylumbelliferyl beta-D-xyloside and of forskolin. Sulfated GAG chains formed in the presence of forskolin had a smaller hydrodynamic radius than controls, as revealed by chromatography on Sepharose CL-6B. Sulfated GAGs from both control and treated cultures behaved identically when chromatographed on DEAE.
authors.affiliation: Department of Biochemistry/Molecular Biology, University of Arkansas for Medical Science, Little Rock.
authors.forename: L M
authors.initials: LM
authors.lastname: Hu
authors.forename: S F
authors.initials: SF
authors.lastname: Kemp
authors.forename: M J
authors.initials: MJ
authors.lastname: Elders
authors.forename: W G
authors.initials: WG
authors.lastname: Smith
mesh_terms: D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D002642:Chick Embryo; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D005576:Colforsin / Q000494:pharmacology*; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006027:Glycosides / Q000494:pharmacology*
chemical_list: D006025:Glycosaminoglycans; D006027:Glycosides; C010807:xylosides; D005576:Colforsin