Fluoroaluminate mimics agonist application in single rat hepatocytes.
Single rat hepatocytes microinjected with the photoprotein aequorin were stimulated with glycogenolytic agonists or low concentrations of fluoroaluminate. Both protocols resulted in the generation of oscillations in cytosolic free Ca2+ levels from a resting value of approx. 200 nM and peaking at over 600 nM. However, oscillations induced by receptor-dependent agonists were more regular in both frequency and time course than those induced by direct activation of G-proteins. The role of G-proteins in the generation of repetitive free Ca2+ oscillations is discussed.
Woods
N M
NM
Department of Human Anatomy and Cell Biology, University of Liverpool, U.K.
Dixon
C J
CJ
Cuthbertson
K S
KS
Cobbold
P H
PH
D000331:Aequorin; D000535:Aluminum / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D005461:Fluorine / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D010656:Phenylephrine / Q000494:pharmacology*; D051381:Rats
D010656:Phenylephrine; C053629:fluoroaluminum; D005461:Fluorine; D000331:Aequorin; D000535:Aluminum; D002118:Calcium