title: Biochemical and immunochemical comparison of Africanized and European honeybee venoms.
abstract: Africanized honeybees (HBs) pose a hazard to both normal and sting-sensitive subjects in certain areas of Central and South America, and it is predicted that they will soon be present in the southern United States as well. Using an electrical stimulation device, we collected Africanized HB venom (AHV) in Venezuela and European HB venom (EHV) in Louisiana. These venoms, along with commercial European HB venom (CHV), were compared by thin-layer isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The Coomassie brilliant blue and silver-stained banding patterns of AHV and EHV were essentially identical to CHV. Western blots were prepared from SDS-PAGE gels and tested with pooled sera from EHV-sensitive subjects and then radiolabeled antihuman IgE. The resulting autoradiographs revealed similar banding patterns among EHV, AHV, and CHV. RAST-inhibition studies were performed with solid-phase CHV and pooled sera from EHV-sensitive subjects. The specific allergenic activities of the three HB venoms (allergy units per milligram of protein) were comparable. By RAST-inhibition assay with solid-phase, highly purified individual venom components, AHV and EHV both contained phospholipase A2, hyaluronidase, and high-molecular-weight allergens. The increased morbidity after Africanized HB stings is likely related to their more defensive behavior during which many bees react by stinging rather than to biochemical or allergenic differences between AHV and EHV.
authors.affiliation: Department of Pediatrics, Mayo Medical School, Mayo Clinic, Rochester, Minn 55905.
authors.forename: D R
authors.initials: DR
authors.lastname: Nelson
authors.forename: A M
authors.initials: AM
authors.lastname: Collins
authors.forename: R L
authors.initials: RL
authors.lastname: Hellmich
authors.forename: R T
authors.initials: RT
authors.lastname: Jones
authors.forename: R M
authors.initials: RM
authors.lastname: Helm
authors.forename: D L
authors.initials: DL
authors.lastname: Squillace
authors.forename: J W
authors.initials: JW
authors.lastname: Yunginger
mesh_terms: D000485:Allergens / Q000032:analysis / Q000276:immunology; D000818:Animals; D001514:Bee Venoms / Q000032:analysis* / Q000276:immunology; D001516:Bees / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D007525:Isoelectric Focusing; D008145:Louisiana; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis; D011852:Radioallergosorbent Test; D013048:Specimen Handling / Q000379:methods; D014687:Venezuela
chemical_list: D000485:Allergens; D001514:Bee Venoms; D011506:Proteins