Experimental induction of neoplasia in the accessory sex organs of male Lobund-Wistar rats.
Experimental induction of neoplasia in the urogenital tract was studied in male Lobund-Wistar rats. Animals were given single 30.0-mg/kg i.v. injections of N-nitroso-N-methylurea (NMU) followed 7 days later by s.c. implantation of a 2.0-cm Silastic capsule containing testosterone propionate (TP). Additional rats were given the NMU or TP treatments individually. Control animals were given a single i.v. injection of saline followed by implantation of an empty Silastic capsule. The Silastic implants for each group were replaced every 2 months. This hormone treatment regimen produced significantly (P less than 0.05) elevated serum testosterone concentrations relative to control for 42 days following implantation. Animals were killed at 92, 177, 259, 361, or 427 days post-NMU injection. A high treatment-related incidence of adenocarcinoma occurred in the dorsal and lateral prostatic lobes of animals given the combined NMU-TP treatment. In addition, a few animals had adenocarcinomas of the coagulating gland or the seminal vesicle. The estimated probability of neoplasia in the accessory sex organs by 427 days after initiation of the NMU-TP treatment was 68%, with no occurrence before 9 months. The NMU-TP treatment was also associated with an incidence of focal dysplasia in the accessory sex organs, particularly in the coagulating gland. These findings indicate that NMU-TP treatment of Lobund-Wistar rats can provide a useful experimental system to study the biochemical and molecular events involved in the induction of accessory sex organ neoplasia.
Hoover
D M
DM
Lilly Research Laboratories, Division of Eli Lilly and Company, Greenfield, Indiana 46140.
Best
K L
KL
McKenney
B K
BK
Tamura
R N
RN
Neubauer
B L
BL
D000230:Adenocarcinoma / Q000139:chemically induced* / Q000473:pathology; D000818:Animals; D005834:Genital Neoplasms, Male / Q000139:chemically induced* / Q000473:pathology; D005837:Genitalia, Male / Q000187:drug effects / Q000473:pathology*; D008297:Male; D008770:Methylnitrosourea / Q000633:toxicity*; D009374:Neoplasms, Experimental / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D012826:Silicone Elastomers; D013739:Testosterone / Q000633:toxicity*
D012826:Silicone Elastomers; D013739:Testosterone; D008770:Methylnitrosourea