Effects of turpentine-induced inflammation on the hypoxic stimulation of intestinal Fe3+ absorption in mice.
Chronic subcutaneous turpentine administration (weekly for 6 weeks) induced a mild normocytic anaemia in mice. In-vitro and in-vivo intestinal Fe3+ absorption parameters were, however, not significantly altered from values in saline-treated or untreated mice. Normal mice, when exposed to 3 days hypoxia demonstrated a 2-3-fold increase in iron absorption in vivo, mainly due to changes in the amount of iron transferred from the mucosa to the plasma and thence to the carcass. A 2-3-fold increase in Vmax was also observed in in-vitro uptake experiments using isolated duodenal fragments. In contrast, turpentine-treated animals, though demonstrating an enhanced in-vitro maximal uptake capacity, failed to elicit an adaptive response in vivo following hypoxic exposure. These findings suggest that a circulating (humoral) factor may be responsible for the inhibition in absorption in vivo in this turpentine-induced inflammatory model.
Raja
K B
KB
Department of Clinical Biochemistry, King's College School of Medicine and Dentistry, Denmark Hill, London, UK.
Duane
P
P
Peters
T J
TJ
D000740:Anemia / Q000139:chemically induced; D000818:Animals; D004386:Duodenum / Q000378:metabolism; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism*; D007408:Intestinal Absorption / Q000502:physiology*; D007413:Intestinal Mucosa / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D051379:Mice; D010100:Oxygen; D014425:Turpentine
D007501:Iron; D010100:Oxygen; D014425:Turpentine