title: Analysis of oxolinic acid in fish by high-performance liquid chromatography.
abstract: A simple high-performance liquid chromatographic method for assaying oxolinic acid, a chemotherapeutic agent, in fish tissues has been developed. Nalidixic acid is used as an internal standard. The drugs are separated on an internal surface reversed-phase column. The sample clean-up is minimized. Serum samples are analysed by direct injection on the column; muscle and liver samples are analysed after solid-phase extraction. The recoveries of oxolinic acid from spiked rainbow trout serum, muscle tissue and liver are 99.7, 87.7 and 83.6%, respectively. The lowest measurable amount of the drug is 0.01 microgram/g in all three tissues.
authors.affiliation: Institute of Parasitology, Abo Akademi, Finland.
authors.forename: H V
authors.initials: HV
authors.lastname: Björklund
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000737:chemistry; D009132:Muscles / Q000737:chemistry; D010093:Oxolinic Acid / Q000032:analysis* / Q000097:blood; D011786:Quality Control; D014337:Trout / Q000378:metabolism*
chemical_list: D010093:Oxolinic Acid