title: Production and significance of TGF-beta in AT-3 metastatic cell line established from the Dunning rat prostatic adenocarcinoma.
abstract: A colony formation assay using NRK-49F cells revealed that a metastatic cell line, AT-3, established from the Dunning prostatic carcinoma could produce TGF-beta in a latent form. TGF-beta at a concentration as low as 0.05 ng/ml either stimulated the attachment or detachment of AT-3 cells depending on the kind of culture media. Acid extracts from conditioned medium (5 micrograms/ml) showed the activity comparable to that of TGF-beta (5 ng/ml). The detached cells were able to grow in suspension. TGF-beta (0.1 ng/ml) could also stimulate the growth of MC3T3-El osteoblasts established from mouse calvaria. These results suggest that TGF-beta is a key growth factor for osteoblastic bony metastasis of prostate cancer.
authors.affiliation: W. Alton Jones Cell Science Center, Inc., Lake Placid, New York 12946.
authors.forename: Y
authors.initials: Y
authors.lastname: Matuo
authors.forename: N
authors.initials: N
authors.lastname: Nishi
authors.forename: H
authors.initials: H
authors.lastname: Takasuka
authors.forename: Y
authors.initials: Y
authors.lastname: Masuda
authors.forename: K
authors.initials: K
authors.lastname: Nishikawa
authors.forename: J T
authors.initials: JT
authors.lastname: Isaacs
authors.forename: P S
authors.initials: PS
authors.lastname: Adams
authors.forename: W L
authors.initials: WL
authors.lastname: McKeehan
authors.forename: G H
authors.initials: GH
authors.lastname: Sato
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D008297:Male; D009362:Neoplasm Metastasis; D009374:Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology; D010006:Osteoblasts / Q000166:cytology; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology; D051381:Rats; D015291:Transforming Growth Factors / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
chemical_list: D015291:Transforming Growth Factors