Expression of the pregnancy-specific beta 1-glycoprotein genes in human testis.
Northern blot analysis with placental pregnancy-specific beta 1-glycoprotein (SP1) cDNA probe showed the presence of SP1 mRNAs in human testis. Presence of translational products of the mRNAs was demonstrated by Western blot analysis with anti-human SP1 antibodies albeit difference in mobilities between the testis and placental proteins was apparent. Screening of human testis cDNA library with placental SP1 probe yielded 4 groups of positive clones. Two groups were identical to human placental SP1 cDNAs previously reported. The other 2 groups consisted of cDNA of incompletely processed mRNAs. These 2 groups were present in high abundance. Sequence analysis suggested that the cDNAs were products of different genes.
Borjigin
J
J
Oklahoma Medical Research Foundation, Oklahoma City 73104.
Tease
L A
LA
Barnes
W
W
Chan
W Y
WY
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D011257:Pregnancy Proteins / Q000235:genetics*; D011268:Pregnancy-Specific beta 1-Glycoproteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D013737:Testis / Q000502:physiology*
D011257:Pregnancy Proteins; D011268:Pregnancy-Specific beta 1-Glycoproteins; D012333:RNA, Messenger; D004247:DNA