Identification of geranylgeranyl-modified proteins in HeLa cells.
Previous studies have shown that animal cells contain isoprenoid-modified proteins and that one of these proteins, lamin B, contains a thioether-linked farnesyl group that is attached to cysteine. In the present study, a novel isoprenoid-modification was identified by labeling HeLa cells with [3H]mevalonic acid and analyzing proteolytic digests of the total cell protein. Radioactive fragments were purified from these digests and treated with Raney nickel. The released, labeled material was analyzed by gas-liquid chromatography (GC) and mass spectrometry (MS). This approach revealed that an all-trans geranylgeranyl group was a major isoprenoid modification.
Farnsworth
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Howard Hughes Medical Institute Laboratory, University of Washington, Seattle 98195.
Gelb
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Glomset
J A
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D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004224:Diterpenes / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D008798:Mevalonic Acid / Q000378:metabolism; D015394:Molecular Structure; D009532:Nickel; D010446:Peptide Fragments; D011506:Proteins / Q000378:metabolism*
D004224:Diterpenes; D010446:Peptide Fragments; D011506:Proteins; D009532:Nickel; D008798:Mevalonic Acid