Development and evaluation of the TD97 measles virus vaccine.
The TD97 strain vaccine virus was prepared from the Tanabe strain measles virus by low-temperature passages in primary cell cultures and ultraviolet (UV) mutagenesis. The TD97 strain exhibited the following characteristics: highly temperature sensitive, neither multiplying nor forming any plaques at 40 degrees C in Vero cells; genetically stable, maintaining high temperature sensitivity after ten successive passages in CE cells at 30 degrees C or 35 degrees C; and M proteins of this virus about 1 KD slower in mobility in SDS-PAGE than that of the Tanabe strain. The TD97 strain was further confirmed to be attenuated by an inoculation test into primate brain. In field trials, 752 healthy children were inoculated with a live virus vaccine prepared with this strain, and the following results were obtained: the seroconversion rate was 97% (517/533), and the average HI antibody titer was 2(5.2). An antibody-increasing effect was also observed in children who were initially seropositive. In children who seroconverted, the rates of fever were 15.7% (55/351) for 37.5 degrees C or higher and 4.0% (14/351) for 39 degrees C or higher. The rash rate was 7.7% (27/351), and the incidence of local reaction was 5.4% (19/351). The TD97 strain is thus considered to be suitable in use for an attenuated measles vaccine.
Suzuki
K
K
Division of Vaccine Development, Chiba Serum Institute, Japan.
Morita
M
M
Katoh
M
M
Kidokoro
M
M
Saika
S
S
Yoshizawa
S
S
Hashizume
S
S
Horiuchi
K
K
Okabe
N
N
Shinozaki
T
T
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D002478:Cells, Cultured; D002490:Central Nervous System / Q000382:microbiology; D002521:Cercopithecus; D002648:Child; D002675:Child, Preschool; D005069:Evaluation Studies as Topic; D006801:Humans; D007223:Infant; D008458:Measles Vaccine / Q000276:immunology / Q000592:standards*; D008459:Measles virus / Q000254:growth & development* / Q000276:immunology / Q000528:radiation effects; D012692:Serial Passage; D013696:Temperature; D014466:Ultraviolet Rays; D014613:Vaccines, Attenuated / Q000276:immunology / Q000592:standards; D014709:Vero Cells; D010948:Viral Plaque Assay
D000914:Antibodies, Viral; D008458:Measles Vaccine; D014613:Vaccines, Attenuated