Acid Tyrode's solution can stimulate parthenogenetic activation of human and mouse oocytes.
Fresh and aged (24 hours after ovulation) human oocytes and recently ovulated mouse oocytes may be activated by exposure to acidified Tyrode's solution. No activation of either type of human oocyte was observed after exposure to hyaluronidase or pronase, but significant numbers of fresh mouse oocytes were activated after exposure to pronase but not to chymotrypsin. The implications of these results for the manipulation of human and mouse eggs in vitro are discussed.
Johnson
M H
MH
Embryo and Gamete Research Group, University of Cambridge, United Kingdom.
Pickering
S J
SJ
Braude
P R
PR
Vincent
C
C
Cant
A
A
Currie
J
J
D000818:Animals; D005260:Female; D015181:Gamete Intrafallopian Transfer; D006801:Humans; D006821:Hyaluronoglucosaminidase; D007150:Immunohistochemistry; D007552:Isotonic Solutions / Q000494:pharmacology*; D051379:Mice; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D010312:Parthenogenesis; D013480:Superovulation
D007552:Isotonic Solutions; C037692:Tyrode's solution; D006821:Hyaluronoglucosaminidase