The detection of Gardnerella vaginalis DNA sequences in uncultured clinical specimens with cloned G. vaginalis DNA as probes.
Cloned Gardnerella vaginalis DNA were selected from a plasmid DNA library constructed with partially restriction endonuclease Hind III-digested genomic DNA of G. vaginalis to serve as DNA probe in detecting G. vaginalis. The level of detection was determined to be approximately 10,000 cells by slot-blot DNA hybridization. This probe DNA will not cross-hybridize with DNA of a number of non-Gardnerella micro-organisms commonly found in female genital tract. The DNA probe-based hybridization test may become a useful tool for the identification of G. vaginalis in uncultured clinical specimens.
Pao
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CC
Department of Biochemistry, Chang Gung Medical College, Taipei, Taiwan, Republic of China.
Lin
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SS
Hsieh
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TT
D015139:Blotting, Southern; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D004274:DNA, Recombinant; D005260:Female; D006194:Gardnerella vaginalis / Q000235:genetics / Q000302:isolation & purification*; D006192:Haemophilus Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D009693:Nucleic Acid Hybridization; D014627:Vaginitis / Q000175:diagnosis* / Q000382:microbiology
D015342:DNA Probes; D004269:DNA, Bacterial; D004274:DNA, Recombinant