Liquid chromatographic determination of three sulfonamides in animal tissue and egg.
Sulfonamides are widely used as a feed additive in animal production in Japan. The present paper is a determination of 3 sulfonamides: sulfamethazine (SMZ), sulfamonomethoxine [SMX, 4-amino-N-(3-methoxypyrazinyl)-benzenesulfonamide], and sulfadimethoxine (SDX) in animal tissue and egg by liquid chromatography (LC). Tissues were extracted with acetonitrile and fat was removed by liquid/liquid partition. The sulfonamides were purified by an ODS cartridge column; then each compound was separated by an ODS LC column and detected at 268 nm. Quantification levels were 0.02 ppm for SMZ and SMX, and 0.04 ppm for SDX; detection limits were 0.01 ppm for SMZ and SMX, and 0.02 ppm for SDX. Calibration curves were linear between 2 and 40 ng for SMZ and SMX, and between 4 and 80 ng for SDX. Recoveries from muscle and egg samples spiked with 1-2 micrograms/10 g were 81-98%.
Horii
S
S
Tokyo Metropolitan Research Laboratory of Public Health, Department of Food Hygiene and Nutrition, Japan.
Momma
C
C
Miyahara
K
K
Maruyama
T
T
Matsumoto
M
M
D000818:Animals; D002417:Cattle; D002645:Chickens; D002853:Chromatography, Liquid; D004350:Drug Residues / Q000032:analysis*; D004531:Eggs / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D009132:Muscles / Q000737:chemistry; D013449:Sulfonamides / Q000032:analysis*; D013552:Swine
D013449:Sulfonamides