Tissue-specific differences in the binding of nuclear proteins to a CCAAT motif in the promoter of the androgen-regulated C3 gene.
The expression of the gene for the C3 polypeptide is confined to the ventral prostate of the male rat and is regulated by androgens. To study the mechanism of this tissue-specific and hormone-dependent regulation we have used DNase-I footprinting and band-shift assays to locate binding sites for nuclear proteins isolated from different tissues. In this paper we present evidence that there are tissue-specific differences in the nuclear proteins that are able to bind to a CCAAT motif and a nuclear factor-I consensus that are present in the promoter of the rat C3 gene. Using competition assays and heat denaturation we show that the CAAT box/enhancer binding protein itself may be one of the transcription factors involved.
Zhang
Y L
YL
Shanghai Institute of Biochemistry, Chinese Academy of Sciences, Shanghai.
Parker
M G
MG
Bakker
O
O
D000727:Androgen-Binding Protein / Q000235:genetics*; D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D022762:CCAAT-Enhancer-Binding Proteins; D002467:Cell Nucleus / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000378:metabolism; D003850:Deoxyribonuclease I; D005786:Gene Expression Regulation / Q000187:drug effects*; D008099:Liver / Q000737:chemistry / Q000648:ultrastructure; D008297:Male; D008969:Molecular Sequence Data; D051839:NFI Transcription Factors; D009687:Nuclear Proteins / Q000378:metabolism*; D009928:Organ Specificity; D049488:Phosphatidylethanolamine Binding Protein; D011401:Promoter Regions, Genetic; D011467:Prostate / Q000737:chemistry / Q000648:ultrastructure; D060789:Prostatein; D051381:Rats; D011919:Rats, Inbred Strains; D060765:Secretoglobins; D013737:Testis / Q000737:chemistry / Q000648:ultrastructure; D014157:Transcription Factors; D014598:Uteroglobin; D051840:Y-Box-Binding Protein 1; D060788:Mammaglobin B
D000727:Androgen-Binding Protein; D000728:Androgens; D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D003850:Deoxyribonuclease I; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D049488:Phosphatidylethanolamine Binding Protein; D060789:Prostatein; D060765:Secretoglobins; D014157:Transcription Factors; D014598:Uteroglobin; D051840:Y-Box-Binding Protein 1; C511482:PEBP1 protein, human; C511484:Scgb1d2 protein, rat; C511483:Scgb1d4 protein, rat; C070856:YBX1 protein, human; C556468:Scgb2a1 protein, rat; D060788:Mammaglobin B