Methotrexate binding to human plasma proteins.
The percentage of methotrexate (MTX) binding to plasma protein was 50.4 +/- 1.9 in healthy subject, and 32.3 +/- 3.6 in patients with cancer (breast carcinoma in most cases). Drugs used in combination with MTX in therapy (vincristine, vinblastine, cyclophosphamide, 5-fluorouracil, prednisone) depress the MTX binding to albumins. MTX binds to two kinds of albumins binding sites, with different binding constants: K1 = 8.88 mM-1 and K2 = 1.76 mM-1.
Skibińska
L
L
Department of Physical Chemistry, Karol Marcinkowski Medical Academy, Poznań, Poland.
Ramlau
C
C
Załuski
J
J
Olejniczak
B
B
D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology; D001798:Blood Proteins / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008727:Methotrexate / Q000097:blood*; D009369:Neoplasms / Q000097:blood / Q000188:drug therapy; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism
D001798:Blood Proteins; D012709:Serum Albumin; D008727:Methotrexate