Androgen binding to ammonium sulfate precipitates of human adipose tissue cytosols.
Although androgens are believed to influence the distribution of human adipose tissue and have been detected in human fat, receptors for these sex hormones have yet to be identified. These studies demonstrate that a high-affinity, limited-capacity binding component for the synthetic androgen methyltrienolone (R1881) exists in ammonium sulfate precipitates of human adipose tissue cytosols. The equilibrium dissociation constant (Kd = 0.1 to 0.4 nmol/L, n = 6) and the number of binding sites (2 to 26 fmol/mg protein, n = 22) are consistent with those reported for androgen receptors in rat prostate, human prostatic carcinoma, MCF-7 cells, and baboon myocardium. The relative steroid-binding specificities of the human adipose tissue androphile (R1881 approximately 5 alpha-dihydrotestosterone greater than testosterone greater than estradiol approximately progesterone much greater than dexamethasone) are similar, but not identical, to those reported for androgen receptors in rat prostate (R1881 greater than 5 alpha-dihydrotestosterone approximately testosterone greater than estradiol greater than progesterone much greater than cortisol) and baboon myocardium (R1881 greater than 5 alpha-dihydrotestosterone greater than testosterone greater than progesterone greater than estradiol much greater than cortisol). The function of the androgen-binding component in human adipose tissue is not known.
Miller
L K
LK
Department of Medicine, Beth Israel Medical Center, New York, NY 10003.
Kral
J G
JG
Strain
G W
GW
Zumoff
B
B
D000273:Adipose Tissue / Q000378:metabolism*; D000645:Ammonium Sulfate; D000728:Androgens / Q000378:metabolism*; D001667:Binding, Competitive; D011232:Chemical Precipitation; D003600:Cytosol / Q000378:metabolism*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D015741:Metribolone / Q000378:metabolism; D014316:Tritium
D000728:Androgens; D014316:Tritium; D015741:Metribolone; D000645:Ammonium Sulfate