Immunological effect of two synthetic peptides containing alanine or D-alanine instead of acetyl group of thymosin beta 4 after treatment of human serum.
Two analogs of thymosin beta 4 the N-terminal acetyl groups of which were substituted by Ala or D-Ala, were synthesized by the solution method and studied for their immunological effect on the impaired blastogenic response of T-lymphocytes isolated from uremic patients after treatment of human serum. One of the synthetic analogs, D-Ala-thymosin beta 4 demonstrated a restorative effect on these patients when incubated in human serum, but the other analog, Ala-thymosin beta 4, showed no restorative effect under the same conditions. These results seem to suggest that D-Ala-thymosin beta 4 increases resistance to proteolytic degradation by exopeptidases more than Ala-thymosin beta 4.
Abiko
T
T
Kidney Research Laboratory, Sendai, Japan.
Sekino
H
H
D000409:Alanine / Q000097:blood* / Q000494:pharmacology; D000595:Amino Acid Sequence; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000097:blood* / Q000494:pharmacology; D013947:Thymosin / Q000031:analogs & derivatives* / Q000097:blood / Q000494:pharmacology; D000099201:Thymosin beta(4)
D000409:Alanine; D010455:Peptides; D013947:Thymosin; D000099201:Thymosin beta(4)