Effects of macrocyclic trichothecene congeners on the viability and mitogenesis of murine splenic lymphocytes.
The effects of several congeners of the macrocyclic class of trichothecene mycotoxins on murine splenic cells in vitro were investigated. The mycotoxins were roritoxin B, myrotoxin B, roridins A, D and E, baccharinoids B4, B5 and B12, 16-hydroxyverrucarin A, and verrucarins A and J. Lymphocytes from CD-1 mice were cultured with each of the mycotoxins for 48 h to assess cytotoxicity. The maximum effect of various trichothecenes produced on cells occurred at concentrations ranging from 10(-6) to 10(-4) M. Mycotoxins had no effect at concentrations ranging from 10(-12) to 10(-7) M. The mitogenic stimulants concanavalin A, lipopolysaccharide, phytohemagglutinin, and pokeweed mitogen were added to splenic lymphocyte cultures along with varying concentrations of selected mycotoxins. Blastogenesis was inhibited at concentrations 2-5 orders of magnitude lower than those which produced lethality on resting lymphocytes.
Hughes
B J
BJ
Toxicology Program, Utah State University, Logan 84322-5600.
Jarvis
B B
BB
Sharma
R P
RP
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D008297:Male; D051379:Mice; D015394:Molecular Structure; D012717:Sesquiterpenes / Q000633:toxicity*; D013154:Spleen / Q000166:cytology* / Q000187:drug effects; D014255:Trichothecenes / Q000633:toxicity*
D012717:Sesquiterpenes; D014255:Trichothecenes