Correlation of leukocyte interleukin-1 production with the stimulation of prostaglandin and tissue factor synthesis by human umbilical vein endothelial cells.
Human leukocyte suspensions (neutrophils 80-85%, monocyte 15-20%) were incubated alone or with cultured human umbilical vein endothelial cells. Leukocytes were either directly added to the endothelial cell cultures or separated from them by a 0.4 micron insert filter. Supernatants or cell lysates were obtained at 0.5, 1, 2, and 4 hours of incubation. Supernatants were assayed for the prostacyclin (PGI2) metabolite 6-keto prostaglandin F1 alpha and prostaglandin E2 (PGE2) by radioimmunoassay and for interleukin-1 (IL-1) by the thymocyte co-mitogen assay. Cell lysates were analyzed for cell-associated procoagulant activity (PCA). Co-incubation of endothelial cells with leukocytes stimulated the synthesis of PGI2, PGE2, and PCA. These biochemical changes correlated partially with the release of IL-1 beta. The results suggest that IL-1 released in monocyte neutrophil co-cultures can produce prothrombotic (increased PCA expression) and inflammatory changes (increased synthesis of vasodilatory and permeability enhancing PGI2 and PGE2) in endothelial cells. Neutrophils may represent a source of the released IL-1 and/or may act to stimulate monocyte release of this cytokine and thus play an important role in vascular pathology by a mechanism unrelated to their more direct cytotoxic activity.
Schaub
R G
RG
Upjohn Company, Kalamazoo, MI 49001.
Dunn
C J
CJ
Deibel
M R
MR
Berger
A E
AE
Wunderlich
D
D
Fleming
W E
WE
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis; D001779:Blood Coagulation Factors / Q000096:biosynthesis; D002478:Cells, Cultured; D015232:Dinoprostone / Q000096:biosynthesis; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D011464:Epoprostenol / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D007962:Leukocytes / Q000378:metabolism*; D011247:Pregnancy; D011453:Prostaglandins / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000378:metabolism; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism
D001779:Blood Coagulation Factors; D007375:Interleukin-1; D011453:Prostaglandins; C045071:leukocyte procoagulant activity; D015121:6-Ketoprostaglandin F1 alpha; D011464:Epoprostenol; D015232:Dinoprostone