Flow cytometric analysis of tissue factor (TF) expression on stimulated monocytes--comparison to procoagulant activity of mononuclear blood cells.
Whereas tissue factor (TF), a 47 kDa transmembrane glycoprotein, is constitutively present in certain tissues such as epithelial tissue, brain, and placenta, it is normally not expressed by cells within the vasculature. However, inflammatory mediators including bacterial lipopolysaccharide (LPS) can stimulate the expression of cell surface procoagulant activity (PCA) on monocytes. In our present study the kinetics (over 24 h) of molecular TF expression on LPS-stimulated monocytes analyzed by flow cytometry corresponds closely to functional PCA of human mononuclear blood cells (MBC). Both PCA and TF expression on monocytes were rapid events reaching their maximum after about 6 h of stimulation. At this time approximately 70-80% of monocytes had also achieved maximum anti-TF MAb receptor density. For certain analytical applications, monitoring of molecular TF expression on monocytes by flow cytometry using anti-TF MAb is favorable because there is no influence by PCA inhibitors.
Luther
T
T
Institute of Pathological Anatomy, Academy of Medicine Dresden, Federal Republic of Germany.
Flössel
C
C
Hietschhold
V
V
Koslowski
R
R
Müller
M
M
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001779:Blood Coagulation Factors / Q000378:metabolism*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D009000:Monocytes / Q000378:metabolism*; D013925:Thromboplastin / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D001779:Blood Coagulation Factors; D008070:Lipopolysaccharides; C045071:leukocyte procoagulant activity; D013925:Thromboplastin