title: Development of radiochemically pure antibodies.
abstract: There are presently three approaches to radiolabeling antibodies: direct radiolabeling; radiolabeling of a chelating agent conjugated to an antibody; and radiolabeling of a chelating agent before conjugation to an antibody. Using either the direct radiolabeling or radiolabeling of a chelating agent conjugated to an antibody has not led to a radiochemically pure 99mTc-labeled protein. High radiochemical purity is obtained using a prelabeled ligand; therefore this method is preferred.
authors.affiliation: Squibb Enstitute for Medical Research, New Brunswick, New Jersey 08903-0191.
authors.forename: W C
authors.initials: WC
authors.lastname: Eckelman
mesh_terms: D000906:Antibodies; D002614:Chelating Agents; D007553:Isotope Labeling / Q000379:methods*; D013667:Technetium
chemical_list: D000906:Antibodies; D002614:Chelating Agents; D013667:Technetium