Characterization of two new monoclonal antibodies against Haemophilus paragallinarum serovar C hemagglutinating antigen.
Two new monoclonal antibodies (MAbs), D6D8D5 and B3E6F9, both directed against Haemophilus paragallinarum serovar C hemagglutinating (HA) antigen, were produced, and characteristics of the MAbs were compared with those of the previously described MAb F2E6 in dot-blot and hemagglutination-inhibition (HI) tests using two representative H. paragallinarum strains each of serovars A, B, and C strains and 55 Japanese serovar C field isolates. MAb D6D8D5 and MAb F2E6 reacted with all serovar C strains and field isolates in the dot-blot test. However, MAb D6D8D5 showed various degrees of inhibition of the HA activity of field isolates. In the enzyme-linked immunosorbent assay-competition test, MAb D6D8D5 did not compete with MAb F2E6. MAb B3E6F9 reacted with strain S1, serovar C but not with strain Modesto, serovar C in both dot-blot and HI tests. Three out of 55 field isolates did not react with MAb B3E6F9. Neither MAb reacted with the serovar A and B strains.
Yamaguchi
T
T
Aburahi Laboratories, Shionogi & Co., Ltd., Shiga, Japan.
Takigami
S
S
Iritani
Y
Y
Hayashi
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D001667:Binding, Competitive; D002850:Chromatography, Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006190:Haemophilus / Q000276:immunology*; D006384:Hemagglutination; D006385:Hemagglutination Inhibition Tests; D015151:Immunoblotting
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial