The stability of glutathione peroxidase activity in plasma from cattle, pigs and sheep on storage in the presence and absence of glutathione.
Ovine, bovine and porcine plasma glutathione peroxidase (GSH-Px) activity decreased on storage at both 4 degrees C and 20 degrees C. The ovine and bovine enzymes were significantly less stable than the porcine enzyme. The addition of GSH to a final concentration of 2 mmol/L to plasma samples at the commencement of storage retarded the loss of both ovine and bovine plasma GSH-Px activity. The ovine enzyme was unique in that after inactivation by storage at 4 degrees C, incubation with GSH restored the enzyme activity. It is recommended that plasma GSH-Px should be assayed fresh, or otherwise stored at -20 degrees C.
Davidson
W B
WB
Veterinary Research Laboratories, Stormont, Belfast, UK.
Kennedy
D G
DG
Hughes
P J
PJ
Blanchflower
W J
WJ
D000818:Animals; D002417:Cattle / Q000097:blood*; D003080:Cold Temperature; D004789:Enzyme Activation; D004795:Enzyme Stability; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000097:blood* / Q000378:metabolism; D006207:Half-Life; D007700:Kinetics; D011309:Preservation, Biological; D012756:Sheep / Q000097:blood*; D013552:Swine / Q000097:blood*
D005979:Glutathione Peroxidase; D005978:Glutathione