Long-term storage of nuclear suspensions prepared from paraffin-embedded material for flow cytometric DNA analysis.
Nuclear suspensions were prepared from archival material of cancer biopsies. DNA content analysis by flow cytometry was performed after storage at -80 degrees C for 24 h and 6 months, compared to 24 h storage at +4 degrees C. No significant variations in CV or DNA indices were observed. Repeated freezing and thawing did not reduce the DNA histogram quality.
Mørkve
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Department of Pathology, Gade Institute, Haukeland Hospital, University of Bergen, Norway.
D002467:Cell Nucleus / Q000737:chemistry*; D015925:Cryopreservation; D004247:DNA / Q000032:analysis*; D005434:Flow Cytometry; D006801:Humans; D010232:Paraffin; D010434:Pepsin A; D013048:Specimen Handling / Q000379:methods*; D013997:Time Factors
D010232:Paraffin; D004247:DNA; D010434:Pepsin A