[Effect of methylguanidine and guanidinosuccinic acid on pyruvate kinase activity in human red cells].
An effect of methylguanidine and guanidinosuccinic acid on pyruvate kinase activity in human red cells was determined in vitro following a 3-hour incubation at 37 degrees C. The obtained results have shown that methylguanidine in the concentration of 1.8 x 10(-5) M/l inhibits pyruvate kinase activity by 20.8%. Pyruvate kinase activity was statistically significantly inhibited on addition of methylguanidine in the concentration of 5.4 x 10(-5) M/l whereas higher concentrations have no such an effect Guanidinesuccinic acid exerted similar but weaker effect on the activity of pyruvate kinase in human red cells. Mixture of methylguanidine (5.4 x 10(-5) m/l) and guanidinesuccinic acid (2.8 x 10(-5) M/l) does not affect pyruvate kinase activity in normal human red cells under identical experimental conditions.
Kopczyński
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Zakładu Analityki Medycznej Katedry Onkologii, Karola Marcinkowskiego w Poznaniu.
Dryl-Rydzyńska
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T
Baczyk
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D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology; D006146:Guanidines / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008760:Methylguanidine / Q000494:pharmacology*; D013386:Succinates / Q000494:pharmacology*
D006146:Guanidines; D013386:Succinates; D008760:Methylguanidine; C001318:guanidinosuccinic acid
Wpływ metyloguanidyny i kwasu guanidynobursztynowego na aktywność kinazy pirogronianowej krwinek czerwonych człowieka.