Gender and gonadectomy influence specific proteins in the cerebellar cortex of the adult rat: a two-dimensional gel electrophoretic analysis.
Two-dimensional polyacrylamide gel electrophoresis was used to determine the influence of gender and gonadectomy on individual proteins of the 105,000 g supernatant (cytosol) of the cerebellar cortex of the adult rat. At age 60 days were gonadectomized or sham-gonadectomized. At age 140 days the rats were sacrificed and the 105,000 g supernatant prepared from the cerebellar cortex. Sham-operated female rats were sacrificed during the proestrus stage of the estrous cycle. Out of 200 proteins identified, 13 were influenced by gender and/or gonadectomy. Five of these proteins were influenced by both gender and gonadectomy. A total of 18 differences were observed. Apparent molecular weights and isoelectric points (pIs) for the 13 proteins ranged from 22,000 to 92,000 Da and pH 4.8-6.1, respectively. Sex differences occurred in the percent volumes (spot density) of 9 proteins between intact male and proestrous female rats. Sex differences in 4 proteins were not influenced by gonadectomy. These differences were presumed to be genetically programmed and/or to reflect the permanent organizational effects of gonadal steroids on the genome. Differences in the remaining 5 proteins depend upon the transient activational effects of the testicular sex steroids, i.e. these gender differences were eliminated by castration. The effects of gonadectomy on individual proteins were gender specific, i.e. proteins affected by ovariectomy were not influenced by castration and vice versa. This suggests intact gonads are required for the specific gender-dependent proteins identified in the cerebellum.(ABSTRACT TRUNCATED AT 250 WORDS)
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Neuroendocrine Research Laboratory, Veterans Affairs Medical Center, North Chicago, IL 60064.
D000818:Animals; D002525:Cerebellar Cortex / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D008297:Male; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000302:isolation & purification / Q000378:metabolism*; D009919:Orchiectomy; D010052:Ovariectomy; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D012737:Sex Factors
D009419:Nerve Tissue Proteins