title: Acid-catalyzed hydrolysis of peptide-amides in the solid state.
abstract: The hydrolysis of peptide-amides in the solid state at 4 degrees due to the presence of residual strong acid was investigated. It was found that even small molar excesses of these acids cause substantial deamidation in one day. The degree of amide hydrolysis depends not only upon the pKa and the volatility of the acid, but also upon the accessibility of the amide function; peptides with bulky C-terminal residues are more stable than the less-hindered ones.
authors.affiliation: Organon Scientific Development Group, Oss, The Netherlands.
authors.forename: P B
authors.initials: PB
authors.lastname: Ten Kortenaar
authors.forename: B M
authors.initials: BM
authors.lastname: Hendrix
authors.forename: J W
authors.initials: JW
authors.lastname: Van Nispen
mesh_terms: D000143:Acids; D000577:Amides / Q000737:chemistry; D000595:Amino Acid Sequence; D003894:Deamino Arginine Vasopressin / Q000737:chemistry; D006868:Hydrolysis; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*
chemical_list: D000143:Acids; D000577:Amides; D010455:Peptides; D003894:Deamino Arginine Vasopressin