Development of a cell culture assay for Bordetella parapertussis heat-labile toxin.
A cell culture assay for heat-labile toxin isolated from Bordetella parapertussis has been developed. In this assay, the ability of heat-labile toxin to induce contraction of vascular smooth muscle cells is measured. The method allows for detection of as little as 0.6 ng/ml of the toxin. The results obtained from this in vitro assay correlated well with those obtained with in vivo assays indicating that the cell culture assay may be a useful alternative to animal assays.
Endoh
M
M
Department of Microbiology, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
Nagai
M
M
Burns
D L
DL
Manclark
C R
CR
Nakase
Y
Y
D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis*; D001681:Biological Assay / Q000379:methods*; D001884:Bordetella; D002478:Cells, Cultured; D005069:Evaluation Studies as Topic; D009119:Muscle Contraction / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D011503:Transglutaminases; D010566:Virulence Factors, Bordetella
D001427:Bacterial Toxins; D010566:Virulence Factors, Bordetella; C060969:dermonecrotic toxin, Bordetella; D011503:Transglutaminases