Coproporphyrinogen oxidase, protoporphyrinogen oxidase and ferrochelatase activities in iron-overloaded and ethanol-treated rats.
The activities of coproporphyrinogen oxidase, protoporphyrinogen oxidase and ferrochelatase in liver homogenates of iron-overloaded and acutely or chronically ethanol-treated rats were assayed by high performance liquid chromatographic methods. Decreased coproporphyrinogen oxidase activity was found in chronically carbonyliron-fed rats while the activities of protoporphyrinogen oxidase and ferrochelatase remained unchanged. Coproporphyrinogen oxidase and protoporphyrinogen oxidase activities in both chronically and acutely ethanol-treated animals were not significantly different from controls. In chronically ethanol-fed rats normal ferrochelatase activity was found.
Li
F
F
Division of Clinical Cell Biology, MRC Clinical Research Centre, Harrow, Middlesex, UK.
Lim
C K
CK
Peters
T J
TJ
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003304:Coproporphyrinogen Oxidase / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology*; D005294:Ferrochelatase / Q000378:metabolism*; D007501:Iron / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology; D008297:Male; D010088:Oxidoreductases / Q000378:metabolism*; D044925:Oxidoreductases Acting on CH-CH Group Donors; D050768:Protoporphyrinogen Oxidase; D051381:Rats; D011919:Rats, Inbred Strains; D013056:Spectrophotometry, Ultraviolet
D000431:Ethanol; D007501:Iron; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; D003304:Coproporphyrinogen Oxidase; D050768:Protoporphyrinogen Oxidase; D005294:Ferrochelatase