Production and significance of TGF-beta in AT-3 metastatic cell line established from the Dunning rat prostatic adenocarcinoma.
A colony formation assay using NRK-49F cells revealed that a metastatic cell line, AT-3, established from the Dunning prostatic carcinoma could produce TGF-beta in a latent form. TGF-beta at a concentration as low as 0.05 ng/ml either stimulated the attachment or detachment of AT-3 cells depending on the kind of culture media. Acid extracts from conditioned medium (5 micrograms/ml) showed the activity comparable to that of TGF-beta (5 ng/ml). The detached cells were able to grow in suspension. TGF-beta (0.1 ng/ml) could also stimulate the growth of MC3T3-El osteoblasts established from mouse calvaria. These results suggest that TGF-beta is a key growth factor for osteoblastic bony metastasis of prostate cancer.
Matuo
Y
Y
W. Alton Jones Cell Science Center, Inc., Lake Placid, New York 12946.
Nishi
N
N
Takasuka
H
H
Masuda
Y
Y
Nishikawa
K
K
Isaacs
J T
JT
Adams
P S
PS
McKeehan
W L
WL
Sato
G H
GH
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D008297:Male; D009362:Neoplasm Metastasis; D009374:Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology; D010006:Osteoblasts / Q000166:cytology; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology; D051381:Rats; D015291:Transforming Growth Factors / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
D015291:Transforming Growth Factors