title: Cell-associated oligosaccharides of Bradyrhizobium spp.
abstract: We report the initial characterization of the cell-associated oligosaccharides produced by four Bradyrhizobium strains: Bradyrhizobium japonicum USDA 110, USDA 94, and ATCC 10324 and Bradyrhizobium sp. strain 32H1. The cell-associated oligosaccharides of these strains were found to be composed solely of glucose and were predominantly smaller than the cyclic beta-1,2-glucans produced by Agrobacterium and Rhizobium species. Linkage studies and nuclear magnetic resonance analyses demonstrated that the bradyrhizobial glucans are linked primarily by beta-1,6 and beta-1,3 glycosidic bonds. Thus, the bradyrhizobia appear to synthesize cell-associated oligosaccharides of structural character substantially different from that of the cyclic beta-1,2-glucans produced by Agrobacterium and Rhizobium species.
authors.affiliation: Department of Food Science, Pennsylvania State University, University Park 16802.
authors.forename: K J
authors.initials: KJ
authors.lastname: Miller
authors.forename: R S
authors.initials: RS
authors.lastname: Gore
authors.forename: R
authors.initials: R
authors.lastname: Johnson
authors.forename: A J
authors.initials: AJ
authors.lastname: Benesi
authors.forename: V N
authors.initials: VN
authors.lastname: Reinhold
mesh_terms: D005936:Glucans / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000032:analysis*; D012230:Rhizobiaceae / Q000032:analysis*; D012231:Rhizobium / Q000032:analysis
chemical_list: D005936:Glucans; D009844:Oligosaccharides