[Isolation of human thrombin by affinity chromatography with silicate to be used in the preparation of biological glue].
At present the preparations of fibrin glue used a thrombin from equine or bovine origin. In order to remove the problems of antigenicity we developed a method of purification from acidified plasma. We used one affinity chromatography on benzamidine-Spherodex and compared three methods of elution: non specific (sodium chloride gradient) and biospecific competitors (arginin methylester or benzamidin). The yield is evaluated between 64 and 84% and the purification factor close to 160. The obtained thrombin is better than animal thrombin in the preparations of fibrin glue.
Allary
M
M
Centre de Transfusion Sanguine des Armées Jean-Julliard, Clamart.
Boschetti
E
E
Lorne
J L
JL
D001550:Benzamidines; D002846:Chromatography, Affinity / Q000379:methods; D015718:Fibrin Tissue Adhesive; D006801:Humans; D012965:Sodium Chloride; D013917:Thrombin / Q000302:isolation & purification*
D001550:Benzamidines; D015718:Fibrin Tissue Adhesive; D012965:Sodium Chloride; D013917:Thrombin
Isolement par chromatographie d'affinité, sur support de silice, de la thrombine humaine en vue de son utilisation dans les préparations de colle biologique.