Acid triacyglycerol lipase inhibitor in chicken plasma: purification and properties.
1. Acid triacylglycerol lipase inhibitor was highly purified from chicken plasma by ammonium sulfate fractionation (0.6-1 saturation) followed by successive chromatographies on Hydroxyapatite, Blue-Cellulofine, Phenyl-Sepharose and Cellulofine GCL-2000 columns, and isoelectric focusing. 2. The lipase inhibitor showed its inhibitory action on triacylglycerol lipases in chicken erythrocytes ghosts and in chicken liver lysosome, but did not on pancreatic lipase, Rhizopus arrhizus lipase, or wheat germ lipase. 3. The inhibitor showed its molecular weight of 32,000 by Cellulofine GCL-2000 gel filtration. The inhibitor showed some heterogeneity on isoelectric focusing, and the main band had a pI of 5.10. 4. The lipase inhibitor did not show any inhibitory action on trypsin or chymotrypsin.
Fujii
M
M
Department of Animal Science, Faculty of Agriculture, Kagoshima University, Japan.
Fukukura
K
K
Kisanuki
K
K
Yamaoka
H
H
Koga
K
K
D000645:Ammonium Sulfate; D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D005591:Chemical Fractionation; D002645:Chickens / Q000097:blood*; D002845:Chromatography; D004910:Erythrocyte Membrane / Q000201:enzymology; D007526:Isoelectric Point; D008049:Lipase / Q000037:antagonists & inhibitors*; D008099:Liver / Q000648:ultrastructure; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D008970:Molecular Weight
D001798:Blood Proteins; D008049:Lipase; D000645:Ammonium Sulfate