title: Alkaline phosphatase production by periosteal cells at various oxygen tensions in vitro.
abstract: A mammalian periosteal cell culture system was developed to investigate the metabolic response of fresh calf bone periosteal cells to various oxygen tensions in vitro. Two predominant cell phenotypes were seen in the culture system. A rapidly proliferating mat of alkaline phosphatase-negative cells supported the growth of overlying clusters of alkaline phosphatase-positive cells. The appearance and subsequent population growth of the alkaline phosphatase-positive cells correlated directly with increases in enzyme activity on biochemical assay. Alkaline phosphatase production was optimal at lower oxygen tensions (5%, 9%), which approximated capillary pO2. In addition, the preconfluence oxygen environment was more critical to the final expression of the enzyme activity than the postconfluence environment. The mechanism of the environmental regulation of alkaline phosphatase gene expression at various oxygen tensions is not known. Periosteal cells were highly sensitive to oxygen tension and expressed alkaline phosphatase enzyme activity at oxygen levels approximating capillary rather than atmospheric pO2.
authors.affiliation: McKay Laboratory of Orthopaedic Surgery Research, University of Pennsylvania School of Medicine, Philadelphia 19104.
authors.forename: J A
authors.initials: JA
authors.lastname: Deren
authors.forename: F S
authors.initials: FS
authors.lastname: Kaplan
authors.forename: C T
authors.initials: CT
authors.lastname: Brighton
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D015870:Gene Expression; D066298:In Vitro Techniques; D010100:Oxygen / Q000494:pharmacology*; D010521:Periosteum / Q000166:cytology / Q000201:enzymology*; D011312:Pressure
chemical_list: D000469:Alkaline Phosphatase; D010100:Oxygen