[Comparative analysis of 2 methods of DNA extraction].
The study of the organization of immunoglobulin and T cell receptor genes in leukaemias and lymphomas depends on our ability to obtain high molecular weight DNA. We have compared two different methods of DNA extraction: one being enzymatic, using the enzyme proteinase K, and the other chemical, using urea as a substance purifying DNA. Since the amount and purity of the DNA obtained are similar with each of the two methods of DNA extraction, both are valid to make such a genetic analysis.
Sánchez García
I
I
Departamento de Medicina, Hospital Clínico Universitario, Salamanca.
Martín Seisdedos
C
C
Pérez Grande
R
R
Corral de la Calle
J
J
Rodríguez Arias
C
C
González-Sarmiento
R
R
D011232:Chemical Precipitation; D004273:DNA, Neoplasm / Q000302:isolation & purification*; D019286:Endopeptidase K; D006801:Humans; D015459:Leukemia-Lymphoma, Adult T-Cell / Q000473:pathology; D008970:Molecular Weight; D012697:Serine Endopeptidases; D014407:Tumor Cells, Cultured / Q000737:chemistry; D014508:Urea
D004273:DNA, Neoplasm; D014508:Urea; D012697:Serine Endopeptidases; D019286:Endopeptidase K
Análisis comparativo de dos métodos de extracción de ADN.