Reinterpretation of linear dichroism of chromatin supports a perpendicular linker orientation in the folded state.
We present a reinterpretation of linear dichroism data for the salt induced condensation of chromatin. A conflict between electric and flow linear dichroism data for identical chromatin samples, studied at varying degrees of Mg2+ induced folding, can be solved if the orientation in electric fields is mainly determined through the polarization of counter ions along the linker parts, whereas the orientation in flow is governed by the hydrodynamical response of the entire chromatin fiber. The orientation of a chromatin fiber in an electric field would then depend on the linker tilt angle so that at an angle larger than 55 degrees the fiber would tend to orient perpendicular to the applied field. The different orientation distributions obtained with the two methods of alignment may in this way provide extra information about the structure and folding of chromatin.
Kubista
M
M
Department of Physical Chemistry, Chalmers University of Technology, Gothenburg, Sweden.
Hagmar
P
P
Nielsen
P E
PE
Nordén
B
B
D002843:Chromatin / Q000737:chemistry*; D004278:DNA, Superhelical / Q000737:chemistry*; D004563:Electrochemistry; D009690:Nucleic Acid Conformation; D011487:Protein Conformation; D013329:Structure-Activity Relationship
D002843:Chromatin; D004278:DNA, Superhelical