Uromodulin: a specific inhibitor of IL-1-initiated human T cell colony formation.
Uromodulin, an 85 kDa naturally occurring immunosuppressant, was found to selectively and specifically inhibit the ability of IL-1 to induce colony responses by highly enriched suspensions of PHA-stimulated T lymphocytes. Dilutions of 1 x 10(-8) M completely blocked the colony growth of T lymphocytes cultured with 50 U/ml IL-1; 1 x 10(-9) M dilutions reduced scores by 83%. By contrast, uromodulin did not inhibit the responses of unseparated mononuclear cells, isolated T lymphocytes cultured with irradiated adherent cells, or stimulated T cells whose growth was initiated by either IL-2 or a soluble factor derived from Raji cells.
Winkelstein
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A
Department of Medicine, Montefiore Hospital, University of Pittsburgh School of Medicine, Pittsburgh 15213.
Muchmore
A V
AV
Decker
J M
JM
Blaese
R M
RM
D003114:Colony-Forming Units Assay; D006801:Humans; D007375:Interleukin-1 / Q000037:antagonists & inhibitors*; D007376:Interleukin-2 / Q000494:pharmacology; D009088:Mucoproteins / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D013491:Suppressor Factors, Immunologic / Q000494:pharmacology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D058949:Uromodulin
D007375:Interleukin-1; D007376:Interleukin-2; D009088:Mucoproteins; D010835:Phytohemagglutinins; D013491:Suppressor Factors, Immunologic; C548612:UMOD protein, human; D058949:Uromodulin