Effects of human 17 kDa interleukin 1, 25-31 kDa thymocyte stimulating activity and the 6-9 kDa interleukin 1 inhibitor on calcium release in the newborn murine calvarial assay.
The effects of human monokines on calcium release from cultured newborn murine calvarium were studied. Highly purified interleukin 1 (IL-1) (17 kDa) and recombinant IL-1 beta in the concentration range 0.2-20 U/ml released significant amounts of calcium. Mean resorption indices (RI) at 0.2 U/ml were 1.28 and 1.49, and at 20 U/ml, were 1.82 and 1.72, respectively. Calcium release was abrogated by the cyclooxygenase inhibitor piroxicam. Thymocyte stimulating activity (TSA) 25-31 kDa alone at 0.14 U/ml released calcium in a prostaglandin dependent manner with a mean RI of 2.13, a significantly greater calcium release than that obtained by 17 kDa IL-1 at 20 U/ml. The 6-9 kDa inhibitor of IL-1 induced thymocyte proliferation alone also released calcium in a prostaglandin dependent manner with a mean RI of 2.29 at 200 inhibitory U/ml. Addition of 6-9 kDa IL-1 inhibitor to the 25-31 kDa material did not significantly change the calcium release, whereas addition of the inhibitor to 17 kDa IL-1 produced a significant increase in calcium release.
Watrous
D A
DA
Division of Rheumatology and Clinical Immunology, University of California, Irvine 92717.
Andrews
B S
BS
Levonian
P J
PJ
Friou
G J
GJ
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002132:Calcium Radioisotopes; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology; D005260:Female; D007375:Interleukin-1 / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D051379:Mice; D015846:Monokines / Q000494:pharmacology*; D010455:Peptides / Q000494:pharmacology*; D011247:Pregnancy; D011453:Prostaglandins / Q000502:physiology; D011994:Recombinant Proteins / Q000494:pharmacology; D012886:Skull / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
D002132:Calcium Radioisotopes; D016207:Cytokines; D007375:Interleukin-1; D015846:Monokines; D010455:Peptides; D011453:Prostaglandins; D011994:Recombinant Proteins; D002118:Calcium