Mutagenic compounds in an extract from Rutae Herba (Ruta graveolens L.). II. UV-A mediated mutagenicity in the green alga Chlamydomonas reinhardtii by furoquinoline alkaloids and furocoumarins present in a commercial tincture from Rutae Herba.
A commercial tincture prepared from Rutae Herba (Ruta graveolens L.) exhibited a moderate photomutagenicity in an arginine-requiring mutant strain of Chlamydomonas reinhardtii. In the tincture some furocoumarins, e.g., bergapten, psoralen, imperatorin, and 3 furoquinoline alkaloids (dictamnine, gamma-fagarine, skimmianine) were detected. All compounds revealed photomutagenic properties but their activities were quite different. Bergapten was the most potent furocoumarin. Dictamnine, the furoquinoline with the strongest effect, reached only about 10% of the activity of bergapten. Based on the amount of these compounds in the tincture and their activities we conclude that bergapten is mainly responsible for the photomutagenicity of the tincture. The lower phototoxicity and photomutagenicity of the furoquinoline alkaloids may be due to the fact that furoquinolines form only monoadducts with DNA in the presence of UV-A in contrast to furocoumarins which also form biadducts.
Schimmer
O
O
Institut für Botanik und Pharmazeutische Biologie, Universität Erlangen-Nürnberg, F.R.G.
Kühne
I
I
D000470:Alkaloids / Q000633:toxicity*; D002696:Chlamydomonas; D004357:Drug Synergism; D011564:Furocoumarins / Q000633:toxicity*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000032:analysis*; D010777:Photochemistry; D010936:Plant Extracts / Q000032:analysis*; D010946:Plants, Medicinal / Q000032:analysis*; D011804:Quinolines / Q000633:toxicity*; D014466:Ultraviolet Rays
D000470:Alkaloids; D011564:Furocoumarins; D009153:Mutagens; D010936:Plant Extracts; D011804:Quinolines; C035932:skimmianine; C049193:gamma-fagarine; C026398:dictamnine