[Simultaneous liquid chromatographic determination of plasma 3,4-dihydroxyphenylglycol and norepinephrine as indicators of sympathetic activity].
A high performance liquid chromatographic method with electrochemical detection is described for simultaneously measuring the concentrations of the endogenous catecholamines and the deaminated catecholamine metabolite 3,4-dihydroxyphenyglycol (DHPG) in plasma. Plasma DHPG concentrations as determined by the present method were 734 +/- 77pg/ml after 30 min. of supine rest whereas plasma norepinephrine (NE) concentrations were 114 +/- 20pg/ml in 5 healthy subjects. The DHPG and NE concentrations increased to 891 +/- 64 and 266 +/- 36pg/ml, respectively, after 30 min. of upright position following 30 min. of supine rest. The simultaneous measurement of plasma DHPG and NE concentrations may provide a more comprehensive assessment of the sympathetic nervous system.
Okumura
K
K
Second Department of Internal Medicine, Nagoya University School of Medicine.
Ito
T
T
D000328:Adult; D001342:Autonomic Nervous System Diseases / Q000175:diagnosis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D008734:Methoxyhydroxyphenylglycol / Q000031:analogs & derivatives* / Q000097:blood; D008875:Middle Aged; D009638:Norepinephrine / Q000097:blood*; D011187:Posture; D013564:Sympathetic Nervous System / Q000502:physiology* / Q000503:physiopathology
D008734:Methoxyhydroxyphenylglycol; C010117:3,4-dihydroxyphenylglycol; D009638:Norepinephrine