title: A rapid two-step purification of rat liver fetal thymidine kinase.
abstract: The fetal isoenzyme of thymidine kinase was purified to apparent homogeneity from cytosols of rat fetuses liver. A two-step purification including anion exchange chromatography and affinity chromatography was developed. The purified enzyme appears as oligomeric with a relative molecular weight of 71 kDa. In denaturing media its molecular weight was 24 kDa, and its pHi 8.3.
authors.affiliation: Centre Régional de Recherche en Endocrinologie, Faculté de Médecine-Pharmacie, Rennes, France.
authors.forename: G
authors.initials: G
authors.lastname: Baron
authors.forename: A
authors.initials: A
authors.lastname: Madec-Baron
authors.forename: S
authors.initials: S
authors.lastname: Sampérez
authors.forename: P
authors.initials: P
authors.lastname: Jouan
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000302:isolation & purification* / Q000378:metabolism; D008099:Liver / Q000196:embryology / Q000201:enzymology*; D008970:Molecular Weight; D051381:Rats; D013937:Thymidine Kinase / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D007527:Isoenzymes; D013937:Thymidine Kinase