Characterization of cerebral energetics and brain pH by 31P spectroscopy after graded canine cardiac arrest and bypass reperfusion.
Recovery of cerebral energy metabolism is used to indicate CNS viability after ischemia. This study utilized 31P nuclear magnetic resonance (NMR) spectroscopy to measure cerebral energy state and intracellular pH in dogs subjected to 8, 12, or 16 min of cardiac arrest and reperfusion using cardiopulmonary bypass. Spectra were obtained throughout ischemia and initial reperfusion and repeated at 30 and 144 h post ischemia. Neurologic deficit scoring was performed at 12 and 24 h post insult and then daily. High-energy phosphates were depleted by the end of all ischemic intervals. Recovery occurred within 60 min of reperfusion and persisted with no differences in the rate of return between groups (p greater than 0.05). Brain pH (pHb) decreased by the end of ischemia in all groups (p less than 0.0001). Neither the pHb nadir nor its recovery differed between groups (p greater than 0.05). Although longterm neurologic outcome differed between groups, the spectra were similar. Assessment of cerebral energy state using 31P NMR spectroscopy does not appear to be a sensitive indicator of neurologic outcome after global ischemia in dogs. Return of high-energy phosphates may be a necessary but not sufficient condition for cerebral recovery after ischemia. The return of high-energy phosphates after a 16-min cardiac arrest, however, indicates a potential for neurological recovery.
Martin
G B
GB
Department of Emergency Medicine, Henry Ford Hospital, Detroit, MI 48202.
Nowak
R M
RM
Paradis
N
N
Rosenberg
J
J
Walton
D
D
Smith
M
M
Eisiminger
R
R
Welch
K M
KM
D000136:Acid-Base Equilibrium / Q000502:physiology*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002315:Cardiopulmonary Bypass; D004285:Dogs; D004734:Energy Metabolism / Q000502:physiology*; D006323:Heart Arrest / Q000378:metabolism* / Q000628:therapy; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D013997:Time Factors