Detection of erythromycin resistance by the polymerase chain reaction using primers in conserved regions of erm rRNA methylase genes.
Genes belonging to different erm DNA hybridization classes were selectively amplified by polymerase chain reaction with a pair of oligonucleotides that corresponded to conserved amino acid motifs in known ERM methylases. Identification of the resistance mechanism was possible despite substantial nucleotide sequence diversity among the erythromycin resistance genes.
Arthur
M
M
Unité des Agents Antibactériens, Institut Pasteur, Paris, France.
Molinas
C
C
Mabilat
C
C
Courvalin
P
P
D000595:Amino Acid Sequence; D001419:Bacteria / Q000187:drug effects* / Q000235:genetics; D003062:Codon; D004352:Drug Resistance, Microbial; D004917:Erythromycin / Q000494:pharmacology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000187:drug effects*
D003062:Codon; D004917:Erythromycin