Solid phase extraction and high performance liquid chromatographic determination of doxophylline in plasma.
A sensitive and selective high performance liquid chromatographic doxophylline assay with ultraviolet detection has been developed for plasma samples. The drug is isolated from biological samples with a reversed phase C18 disposable extraction column. Plasma standard curves are linear for concentrations of doxophylline from 0.03 to 10 mg/L. At the therapeutic range concentrations, the recoveries are better than 96.9%. The coefficients of variation for the procedure are 4.1% and 2.7% for the concentrations 0.03 mg/L and 10 mg/L, respectively. By this method, pharmacokinetic profiles are obtained for six adult volunteers.
Lagana
A
A
Department of Industrial Chemistry, University of Bologna, Italy.
Bizzarri
M
M
Marino
A
A
Mancini
M
M
D000328:Adult; D000996:Antitussive Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D013056:Spectrophotometry, Ultraviolet; D013806:Theophylline / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics
D000996:Antitussive Agents; D007202:Indicators and Reagents; D013806:Theophylline; C008749:etofylline; C029797:doxofylline