Effect of forskolin on synthesis of xyloside-initiated glycosaminoglycans in embryonic chick chondrocytes.
The synthesis of sulfated glycosaminoglycan (GAG) chains has been studied in the presence of various concentrations of the artificial acceptor 4-methylumbelliferyl beta-D-xyloside and of forskolin. Sulfated GAG chains formed in the presence of forskolin had a smaller hydrodynamic radius than controls, as revealed by chromatography on Sepharose CL-6B. Sulfated GAGs from both control and treated cultures behaved identically when chromatographed on DEAE.
Hu
L M
LM
Department of Biochemistry/Molecular Biology, University of Arkansas for Medical Science, Little Rock.
Kemp
S F
SF
Elders
M J
MJ
Smith
W G
WG
D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D002642:Chick Embryo; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D005576:Colforsin / Q000494:pharmacology*; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006027:Glycosides / Q000494:pharmacology*
D006025:Glycosaminoglycans; D006027:Glycosides; C010807:xylosides; D005576:Colforsin