title: A comparison of the results of sequential hydrazinolysis-nitrosation and alkali-mediated cleavage-nitrosation of the O-linked oligosaccharides of gastric mucus glycoproteins.
abstract: Analysis of the oligosaccharides released from pig gastric mucus glycopolypeptides by hydrazinolysis showed that degradation had occurred. Nitrosation of the products followed by reduction gave a mixture that had a low content of 2,5-anhydro-D-talitol, which implied destruction of much of the terminal reducing 2-amino-2-deoxy-D-galactose. Under the conditions of hydrazinolysis, cellobiose was largely unchanged but laminaribiose gave a complex mixture that probably contained glucose hydrazone (13C-n.m.r. data). In order to avoid degradation, the hydrazinolysis-nitrosation sequence should be applied to the reduced oligosaccharides released on cleavage with alkali.
authors.affiliation: Chemistry Department, University College of Swansea, Great Britain.
authors.forename: S R
authors.initials: SR
authors.lastname: Carter
authors.forename: J M
authors.initials: JM
authors.lastname: Williams
authors.forename: J R
authors.initials: JR
authors.lastname: Clamp
mesh_terms: D000468:Alkalies; D000818:Animals; D002240:Carbohydrate Sequence; D005753:Gastric Mucosa / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry*; D006834:Hydrazines; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015538:Nitrosation; D009844:Oligosaccharides / Q000737:chemistry*; D013552:Swine
chemical_list: D000468:Alkalies; D006023:Glycoproteins; D006834:Hydrazines; D009844:Oligosaccharides; C047874:gastric mucus glycoproteins