Use of water-soluble 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide for the fluorescent determination of uronic acids and carboxylic acids.
Reaction between glucuronic acid and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) was monitored by the o-phthalaldehyde (OPA) method, which was developed for the fluorescent assay of compounds containing an amino group. About 1 nmol of glucuronic acid was detected by this method. This EDC-OPA method was effective in detecting not only acidic sugar but also carboxylic acid. Although the sensitivity of the EDC-OPA method was somewhat lower than that of amino acid determination by OPA, a very simple and convenient assay was attained for compounds containing a carboxyl group.
Kobayashi
M
M
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University, Miyagi, Japan.
Ichishima
E
E
D002241:Carbohydrates / Q000032:analysis; D002264:Carboxylic Acids / Q000032:analysis*; D002845:Chromatography / Q000379:methods; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000737:chemistry*; D005453:Fluorescence; D005965:Glucuronates / Q000032:analysis / Q000737:chemistry; D020723:Glucuronic Acid; D014574:Uronic Acids / Q000032:analysis*; D009764:o-Phthalaldehyde
D002241:Carbohydrates; D002264:Carboxylic Acids; D005965:Glucuronates; D014574:Uronic Acids; D009764:o-Phthalaldehyde; D020723:Glucuronic Acid; D005022:Ethyldimethylaminopropyl Carbodiimide