title: Stimulatory and inhibitory actions of proteins and amino acids on copper-catalysed free radical generation in the bulk phase.
abstract: The effect of a variety of proteins and amino acids was investigated on oxygen free radical activity as assessed by copper/hydrogen peroxide induced benzoate hydroxylation as well as copper-catalysed ascorbate autoxidation. Serum albumins from a variety of species (human, bovine and dog) had both inhibitory and stimulatory effects depending on the molar copper to protein ratio; low ratios were inhibitory and high stimulatory. Some other proteins tested (lysozyme, soybean trypsin inhibitor and conalbumin) also had dual (inhibitory and stimulatory) effects, as did both histidine and polyhistidine, but all effects occurred at different molar ratios presumably dependent on the relative affinities for the copper ions. In contrast, metallothionein and caeruloplasmin, proteins specialised to bind copper in vivo had no stimulatory effects. In this paper we show that in addition to their fairly well documented inhibitory effects, under certain conditions some proteins also stimulate radical reactions. The possible role of this phenomenon in vivo is discussed.
authors.affiliation: Heart Research Institute, Sydney, New South Wales, Australia.
authors.forename: J A
authors.initials: JA
authors.lastname: Simpson
authors.forename: R T
authors.initials: RT
authors.lastname: Dean
mesh_terms: D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism*; D001565:Benzoates / Q000378:metabolism*; D019817:Benzoic Acid; D002417:Cattle; D002570:Ceruloplasmin / Q000494:pharmacology; D003207:Conalbumin / Q000494:pharmacology; D003300:Copper / Q000378:metabolism*; D004285:Dogs; D005609:Free Radicals; D006639:Histidine / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008668:Metallothionein / Q000494:pharmacology; D009113:Muramidase / Q000494:pharmacology; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D010455:Peptides / Q000494:pharmacology; D011506:Proteins / Q000494:pharmacology*; D012709:Serum Albumin / Q000494:pharmacology; D014361:Trypsin Inhibitors / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D001565:Benzoates; D005609:Free Radicals; D010455:Peptides; D011506:Proteins; D012709:Serum Albumin; D014361:Trypsin Inhibitors; D003207:Conalbumin; C033223:polyhistidine; D006639:Histidine; D003300:Copper; D019817:Benzoic Acid; D008668:Metallothionein; D006861:Hydrogen Peroxide; D002570:Ceruloplasmin; D009113:Muramidase; D001205:Ascorbic Acid; D010100:Oxygen