title: Effects of macrocyclic trichothecene congeners on the viability and mitogenesis of murine splenic lymphocytes.
abstract: The effects of several congeners of the macrocyclic class of trichothecene mycotoxins on murine splenic cells in vitro were investigated. The mycotoxins were roritoxin B, myrotoxin B, roridins A, D and E, baccharinoids B4, B5 and B12, 16-hydroxyverrucarin A, and verrucarins A and J. Lymphocytes from CD-1 mice were cultured with each of the mycotoxins for 48 h to assess cytotoxicity. The maximum effect of various trichothecenes produced on cells occurred at concentrations ranging from 10(-6) to 10(-4) M. Mycotoxins had no effect at concentrations ranging from 10(-12) to 10(-7) M. The mitogenic stimulants concanavalin A, lipopolysaccharide, phytohemagglutinin, and pokeweed mitogen were added to splenic lymphocyte cultures along with varying concentrations of selected mycotoxins. Blastogenesis was inhibited at concentrations 2-5 orders of magnitude lower than those which produced lethality on resting lymphocytes.
authors.affiliation: Toxicology Program, Utah State University, Logan 84322-5600.
authors.forename: B J
authors.initials: BJ
authors.lastname: Hughes
authors.forename: B B
authors.initials: BB
authors.lastname: Jarvis
authors.forename: R P
authors.initials: RP
authors.lastname: Sharma
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects*; D008297:Male; D051379:Mice; D015394:Molecular Structure; D012717:Sesquiterpenes / Q000633:toxicity*; D013154:Spleen / Q000166:cytology* / Q000187:drug effects; D014255:Trichothecenes / Q000633:toxicity*
chemical_list: D012717:Sesquiterpenes; D014255:Trichothecenes