title: Separation of glycoprotein-N-glycans by high-pH anion-exchange chromatography.
abstract: A series of high-mannose and complex type glycoprotein-N-glycans was subjected to high-pH anion-exchange chromatography. The results revealed that this method represents a useful tool for analytical characterization of single oligosaccharides as well as preparative separation of complex mixtures of carbohydrate side-chains. On the other hand, it became evident that in several cases a combination of different chromatographic techniques is required for efficient separation of individual oligosaccharide species.
authors.affiliation: Biochemisches Institut am Klinikum, Universität Giessen, FRG.
authors.forename: G
authors.initials: G
authors.lastname: Pfeiffer
authors.forename: H
authors.initials: H
authors.lastname: Geyer
authors.forename: R
authors.initials: R
authors.lastname: Geyer
authors.forename: I
authors.initials: I
authors.lastname: Kalsner
authors.forename: P
authors.initials: P
authors.lastname: Wendorf
mesh_terms: D002852:Chromatography, Ion Exchange; D006023:Glycoproteins / Q000302:isolation & purification*; D006596:Hexosaminidases; D006863:Hydrogen-Ion Concentration; D008745:Methylation; D009844:Oligosaccharides / Q000302:isolation & purification; D011134:Polysaccharides / Q000302:isolation & purification*
chemical_list: D006023:Glycoproteins; D009844:Oligosaccharides; D011134:Polysaccharides; D006596:Hexosaminidases