Tin etiopurpurin dichloride-sensitized lipid photooxidation of erythrocyte membranes.
Tin(IV) etiopurpurin dichloride (SnET2 x 2Cl) is a photosensitizer which has been shown to be an effective photodynamic agent for the treatment of transplantable animal tumors in vivo. The purpose of this study was to understand the effect of SnET2 x 2Cl on membrane lipid peroxidation. When erythrocyte membranes were exposed to visible light in the presence of SnET2 x 2Cl, lipid peroxidation was observed. An accumulation of lipid hydroperoxides and an increase in lipid fluorescence were also observed. Thin layer chromatography of lipid extracts from photooxidized membrane revealed photoperoxide products derived from phospholipid. Investigations into the mechanism(s) of lipid peroxidation by SnET2 x 2Cl and light-sensitized membranes were also performed. Results indicate that singlet oxygen (1O2) plays a major role in lipid peroxidation.
Morgan
A R
AR
Department of Chemistry, University of Toledo, OH 43606.
Cheng
L S
LS
Skalkos
D
D
Garbo
G M
GM
D000818:Animals; D004285:Dogs; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D015227:Lipid Peroxidation / Q000187:drug effects*; D008563:Membrane Lipids / Q000097:blood; D010777:Photochemistry; D011166:Porphyrins / Q000494:pharmacology; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*
D008563:Membrane Lipids; D011166:Porphyrins; D011838:Radiation-Sensitizing Agents; C063166:tin etiopurpurin