Serodiagnosis of respiratory syncytial virus (RSV) infection in children as measured by detection of RSV-specific immunoglobulins G, M, and A with enzyme-linked immunosorbent assay.
The diagnostic value of an enzyme-linked immunosorbent assay for detection of respiratory syncytial virus (RSV)-specific immunoglobulin G (IgG), IgM, and IgA in sera from infants and children with proven RSV infection, from a control group, and from patients with symptoms of viral respiratory disease was analyzed. Compared to virus isolation and RSV antigen detection methods, the sensitivity of this assay was 87% and the specificity was 79%. For IgG alone, these were 45 and 92%, for IgM alone they were 48 and 92%, and for IgA alone they were 74 and 95%, respectively.
Meddens
M J
MJ
Department of Pathology, Clinical Microbiology and Clinical Immunology, Stichting Samenwerking Delftse Ziekenhuizen, Delft, The Netherlands.
Herbrink
P
P
Lindeman
J
J
van Dijk
W C
WC
D000914:Antibodies, Viral / Q000302:isolation & purification*; D000956:Antigens, Viral / Q000302:isolation & purification; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D006801:Humans; D007070:Immunoglobulin A / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000302:isolation & purification; D007223:Infant; D012136:Respiratory Syncytial Viruses / Q000276:immunology* / Q000302:isolation & purification; D010253:Respirovirus Infections / Q000175:diagnosis* / Q000276:immunology; D012698:Serologic Tests
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M