Serum IgG from an autoimmune prone mouse C3H/HeJ-gld/gld supports the interleukin-3-dependent cell line through an autocrine mechanism.
C3H/HeJ-gld/gld(C3H/gld) mice have been shown to develop massive lymphadenopathy with autoimmunity. In this study, we tested whether C3H/gld-IgG supports the growth of the IL-3-dependent cell line, FDC-P2/185-4. Serum IgG from C3H/gld mice stimulated FDC-P2/185-4 cells to proliferate. On the other hand, IgG from C3H/HeJ-+/+ did not show such activity. This activity increased with age in both sexes of C3H/gld mice. It was suggested that a monomeric IgG component was responsible for the proliferative activity of C3H/gld mouse sera. The cell-induced growth required Fc gamma receptors on FDC-P2/185-4 cells. FDC-P2/185-4 cells stimulated with C3H/gld-IgG, secreted IL-3, and grew by themselves, indicating an autocrine mechanism. Thus, cytokines produced by serum IgG may play an important role in the development of disease in mice bearing the autosomal recessive mutation gld.
Iwamoto
M
M
Third Department of Internal Medicine, Yamanashi Medical College, Japan.
Akasu
F
F
Sakihama
T
T
Onaya
T
T
Nakajima
Y
Y
Tasaka
K
K
D000367:Age Factors; D000818:Animals; D001327:Autoimmune Diseases / Q000276:immunology*; D001681:Biological Assay; D007074:Immunoglobulin G / Q000276:immunology; D007160:Immunoproliferative Disorders / Q000276:immunology*; D007377:Interleukin-3 / Q000096:biosynthesis*; D051379:Mice; D008817:Mice, Mutant Strains; D011961:Receptors, Fc / Q000276:immunology
D007074:Immunoglobulin G; D007377:Interleukin-3; D011961:Receptors, Fc