L1, a major granulocyte protein; isolation of high quantities of its subunits.
L1 is a major granulocyte and monocyte protein with a Mr of 36.5 kDa. It is found mainly in the cytosol of these cells. Purified L1 is shown, on two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), to contain three subunits. In this study, 6 mol/l concentration of urea was found to be sufficient for disassembly of the polypeptides, and urea-containing preparative isoelectric focusing gel was used for separation of high quantities of the subunits. The pI of the eluted subunits were 5.8, 6.1 and 7.1. When tested on 2D-PAGE, the isolated subunits were found at their typical locations. Polyclonal rabbit antibodies were produced against the subunits, and the antisera were, on dot-blot, found to react with the different subunits as well as the purified L1.
Berntzen
H B
HB
Institute of Clinical Biochemistry, Rikshospitalet, Oslo, Norway.
Fagerhol
M K
MK
D000954:Antigens, Surface / Q000737:chemistry / Q000302:isolation & purification*; D001798:Blood Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D015180:Electrophoresis, Gel, Two-Dimensional; D006098:Granulocytes / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D007526:Isoelectric Point; D039841:Leukocyte L1 Antigen Complex; D011487:Protein Conformation
D000954:Antigens, Surface; D001798:Blood Proteins; D039841:Leukocyte L1 Antigen Complex