Fatty acid incorporation into membranes of dispersed rat submandibular salivary gland cells and their effect on adenylate cyclase activity.
Dispersed cells from rat submandibular salivary glands were incubated in medium supplemented with different fatty acids, such as stearic (18:0), oleic (cis 18:1 omega 9) and elaidic acid (trans 18:1 omega 9). The exogenous fatty acids were incorporated into the membrane lipids. A comparison of the adenylate cyclase activity in membranes of cells incubated with different fatty acids revealed the following order (from highest to lowest specific activity): stearic, elaidic, oleic. The findings suggest that changes in adenylate cyclase activity in these dispersed membranes enriched with different fatty acids may be related to the possible changes in membrane fluidity. The synthesis of glycoproteins was studied by measuring the incorporation of [1-14C]-glucosamine into TCA-PTA-precipitable material in the presence or absence of fatty acids. No effect of the exogenous fatty acids on glycoprotein synthesis was observed.
Ahmad
S N
SN
Louisiana State University Medical Center, Department of Biochemistry and Molecular Biology, New Oreleans 70119.
Alam
S Q
SQ
Alam
B S
BS
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D008401:Gas Chromatography-Mass Spectrometry; D005944:Glucosamine / Q000493:pharmacokinetics; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D008563:Membrane Lipids / Q000032:analysis / Q000378:metabolism*; D019301:Oleic Acid; D009829:Oleic Acids / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012469:Salivary Glands / Q000166:cytology* / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate; D013229:Stearic Acids / Q000032:analysis / Q000378:metabolism*
D002250:Carbon Radioisotopes; D008562:Membrane Glycoproteins; D008563:Membrane Lipids; D009829:Oleic Acids; D013229:Stearic Acids; D019301:Oleic Acid; D012967:Sodium Dodecyl Sulfate; C011459:elaidic acid; C031183:stearic acid; D000262:Adenylyl Cyclases; D005944:Glucosamine