[Determination of cyclamate in complex matrix using HPLC after column derivatization with 4-fluoro-7-nitrobenzofurazan].
A method for the analysis of cyclamate in complex foodstuffs has been developed. This method is applicable in strongly coloured and protein-rich foodstuffs. The quantitative determination depends on oxidation of cyclamate to cyclohexylamine and derivatisation with 4-fluoro-7-nitrobenzofuran (NBD-F). The derivatives are analysed by HPLC on a C18: reversed-phase column, their minimal stability being 12 h. There are two possible methods of detection: (a) absorbance at 485 nm and (b) fluorescence with excitation at 485 nm and emission at 530 nm. The detection limit of cyclamate is 5 mg/kg foodstuff, with fluorescence detection 0.4 mg/kg. The recoveries are in the range of 88% to 104%.
Hauck
M
M
Chemische Landesuntersuchungsanstalt Stuttgart, Bundesepublik Deutschland.
Köbler
H
H
D009327:4-Chloro-7-nitrobenzofurazan / Q000031:analogs & derivatives*; D002851:Chromatography, High Pressure Liquid; D003494:Cyclamates / Q000032:analysis*; D003514:Cyclohexylamines / Q000378:metabolism; D005456:Fluorescent Dyes; D005504:Food Analysis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction
D003494:Cyclamates; D003514:Cyclohexylamines; D005456:Fluorescent Dyes; C032930:7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole; D009327:4-Chloro-7-nitrobenzofurazan
Bestimmung von Cyclamat in komplexer Matrix mittels HPLC nach Vorsäulenderivatisierung mit 4-Fluoro-7-nitrobenzofurazon.