Reconstituted basement membrane enhances neurite outgrowth in PC12 cells induced by nerve growth factor.
Rat pheochromocytoma cells (PC12) respond to nerve growth factor (NGF) by elaborating neurites. We have studied the effect of extracellular matrix proteins on responsiveness of PC12 cells to NGF. NGF elicited neurite outgrowth in cells plated on collagen, fibronectin, or laminin within 48-72 h. In contrast, cells entrapped in reconstituted basement membrane prepared from the Engelbreth-Holm-Swarm tumor (EHS-BM) responded within 24 h. Cells plated on collagen or fibronectin required a minimal dose of 50 ng/ml NGF to achieve the same effect as cells entrapped in EHS-BM at 1 ng/ml NGF. Neurites displayed by cells plated in EHS-BM were more extensively branched and remained intact up to 21 days following a single administration of NGF.
Schwarz
M A
MA
Department of Cell Biology, Schering-Plough Research, Bloomfield, New Jersey 07003.
Mitchell
M
M
Emerson
D L
DL
D000310:Adrenal Gland Neoplasms / Q000473:pathology*; D000818:Animals; D001485:Basement Membrane / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects; D003094:Collagen; D046508:Culture Techniques / Q000379:methods; D005353:Fibronectins; D007797:Laminin; D009414:Nerve Growth Factors / Q000494:pharmacology*; D010641:Phenotype; D010673:Pheochromocytoma / Q000473:pathology*; D051381:Rats; D013268:Stimulation, Chemical; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000648:ultrastructure
D005353:Fibronectins; D007797:Laminin; D009414:Nerve Growth Factors; D003094:Collagen