In vitro survival of cells derived from isodiploid uniparental half embryos of the mouse after aggregation with normal embryos.
Fertilized mouse eggs, heterozygous for two allozymes of glucose phosphate isomerase (GPI) were bisected, and the resulting half eggs were diploidized with cytochalasin B. After separate aggregation with normal embryos carrying a third allozyme of GPI, the resulting chimaeras were kept in culture up to 10 days. The majority grew out on the culture dish during this period. By GPI analysis, 7.7% of the embryos were found to be chimaeric. Both types of uniparental cells, from gynogenetic and from androgenetic half eggs, were capable of surviving in chimaeras in vitro. These results are comparable with published data obtained by using uniparental embryos generated by micromanipulation.
Koch-Albrecht
T
T
Fachbereich Biologie der Philipps-Universität Marburg, Federal Republic of Germany.
Petzoldt
U
U
D000818:Animals; D002449:Cell Aggregation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D002678:Chimera / Q000235:genetics / Q000502:physiology; D003571:Cytochalasin B / Q000494:pharmacology; D004171:Diploidy; D004622:Embryo, Mammalian / Q000166:cytology / Q000201:enzymology / Q000502:physiology*; D005314:Embryonic and Fetal Development / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D005260:Female; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics / Q000378:metabolism; D006579:Heterozygote; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice
D007527:Isoenzymes; D003571:Cytochalasin B; D005956:Glucose-6-Phosphate Isomerase