The high performance liquid chromatography of enzyme systems relating to purine and pyrimidine metabolism: an overview.
Purines and pyrimidines are now routinely separated by HPLC. By careful selection of chromatographic conditions which match the expected changes in hydrophobicity and/or ionic nature of the substrate and products most enzymes of the purine and pyrimidine salvage pathways can be routinely and accurately determined. Ion-paired reversed-phase systems are often the most advantageous. The relevance of such assays to biomedical analysis including their role in the diagnosis of inborn errors of metabolism is stressed.
Perrett
D
D
Department of Medicine, St. Bartholomew's Hospital Medical College, West Smithfield, London, UK.
Simmonds
H A
HA
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry; D004798:Enzymes / Q000378:metabolism*; D011687:Purines / Q000378:metabolism*; D011743:Pyrimidines / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D004798:Enzymes; D011687:Purines; D011743:Pyrimidines