Ammonia and monoamine concentrations in two brain areas in rats after one hyperoxic seizure.
Monoamines (catecholamines, serotonin, and metabolites) and ammonia were studied within two areas of the rat brain--the frontal cortex (FC) and the striatum (SA)--after exposure to hyperbaric oxygen (HBO) at 6 ATA up to the first seizure. An increase of norepinephrine (NE), dopamine (DA), and metabolites (HVA, DOPAC) measured by the HPLC/EC method were found in SA with a parallel increase of ammonia at variance with the FC where no monoamine changes, but a slight increase of ammonia, were found. Blood ammonia did not change with HBO. So, 20 min after one HBO seizure, there are regional differences in the brain, which are consistent with the previous findings of an SA start of electrocortical abnormalities at the onset of a seizure. Elevated DA, and possibly NE, levels may contribute to the accumulation of ammonia in the brain. During prolonged HBO exposure, this rise of ammonia could be one of the mechanisms involved in the relapse of seizures. It might also be implicated in initiation of the first seizure. By their situations and contents, SA glial cells could play an important role in brain HBO susceptibility.
Mialon
P
P
Laboratoire de Physiologie, UA-CNRS 648, Brest, France.
Caroff
J
J
Barthelemy
L
L
Bigot
J C
JC
D000641:Ammonia / Q000097:blood*; D000818:Animals; D002395:Catecholamines / Q000097:blood*; D003342:Corpus Striatum; D005625:Frontal Lobe / Q000032:analysis*; D006931:Hyperbaric Oxygenation / Q000009:adverse effects*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000097:blood*; D014793:Visual Cortex / Q000032:analysis*
D002395:Catecholamines; D012701:Serotonin; D000641:Ammonia