Structures of the asparagine-linked oligosaccharides of an alkaline phosphatase, kasahara isozyme, purified from FL amnion cells.
Asparagine-linked oligosaccharides were quantitatively released by hydrazinolysis from an alkaline phosphatase, Kasahara isozyme, which was purified from FL amnion cells. Almost all of the oligosaccharides (98%) were acidic components, all of which can be converted to neutral oligosaccharides upon sialidase digestion. Structural analysis of the oligosaccharides by sequential exoglycosidase digestion in combination with methylation analysis revealed that the alkaline phosphatase of FL cells contains sialylated mono-, bi-, tri-, and tetraantennary complex type sugar chains with the Gal beta 1----4GlcNAc beta 1---- outer chains. Some of the tetraantennary sugar chains contain a single Gal beta 1----4GlcNAc beta 1----3Gal beta 1----4GlcNAc beta 1---- outer chain on their Man alpha 1----6 arm. Both fucosylated and nonfucosylated trimannosyl cores were found in the sugar chains. However, it is of interest that the core portion of monoantennary oligosaccharide was not fucosylated and that of the tetraantennary oligosaccharide with a tetrasaccharide outer chain was completely fucosylated.
Endo
T
T
Department of Biochemistry, University of Tokyo, Japan.
Higashino
K
K
Hada
T
T
Imanishi
H
H
Muratani
K
K
Kochibe
N
N
Kobata
A
A
D000469:Alkaline Phosphatase / Q000032:analysis*; D000650:Amnion / Q000166:cytology / Q000201:enzymology; D002240:Carbohydrate Sequence; D002460:Cell Line; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D008745:Methylation; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis*
D007527:Isoenzymes; D009844:Oligosaccharides; D000469:Alkaline Phosphatase