title: Termination region in rRNA genes from a eucaryotic thermophile, Thermomyces lanuginosus.
abstract: S1 mapping of the termination region in the ribosomal DNA from a thermophilic fungus, Thermomyces lanuginosus, revealed three distinct termini corresponding to the mature 25S rRNA, a precursor that is 19 nucleotides longer and corresponds to the 37S precursor in yeast cells, and a putative termination site at +96 that bears a limited sequence homology with the SalI box of mammalian cells. An estimate of the secondary structure suggested that the three termini are in close proximity, a feature that may be essential to precursor termination and maturation. The results raise questions regarding recently reported relationships between ribosomal DNA termination and spacer enhancer elements in fungi.
authors.affiliation: Department of Molecular Biology and Genetics, University of Guelph, Ontario, Canada.
authors.forename: K
authors.initials: K
authors.lastname: Walker
authors.forename: W M
authors.initials: WM
authors.lastname: Wong
authors.forename: R N
authors.initials: RN
authors.lastname: Nazar
mesh_terms: D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D005658:Fungi / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics*; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D012335:RNA, Ribosomal / Q000235:genetics*; D015183:Restriction Mapping; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal