title: Determination of temafloxacin, sarafloxacin, and difloxacin in bulk drug and dosage forms by high-performance liquid chromatography.
abstract: The fluoroquinolones, temafloxacin, sarafloxacin, and difloxacin, are determined in the bulk drug substances and in a variety of dosage form using high-performance liquid chromatography (HPLC). The HPLC system used is also applicable for ciprofloxacin and norfloxacin. The procedure uses UV detection at 280 nm, which provides a linear response of the subject compounds to at least 20 micrograms/ml. Assay precision (RSD) values were +/- 1.2% or better for the bulk drugs and ranged from +/- 0.42 to +/- 2.3% for suspension, capsule, and tablet formulations. Drug recoveries were quantitative from the dosage forms tested. Sensitivity of the subject compounds is approximately 50 ng/ml (2.5 ng on column).
authors.affiliation: Analytical Research Department, Abbott Laboratories, North Chicago, Illinois 60064-4000.
authors.forename: J F
authors.initials: JF
authors.lastname: Bauer
authors.forename: L
authors.initials: L
authors.lastname: Elrod
authors.forename: J R
authors.initials: JR
authors.lastname: Fornnarino
authors.forename: D E
authors.initials: DE
authors.lastname: Heathcote
authors.forename: S K
authors.initials: SK
authors.lastname: Krogh
authors.forename: C L
authors.initials: CL
authors.lastname: Linton
authors.forename: B J
authors.initials: BJ
authors.lastname: Norris
authors.forename: J E
authors.initials: JE
authors.lastname: Quick
mesh_terms: D000890:Anti-Infective Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002939:Ciprofloxacin / Q000009:adverse effects / Q000031:analogs & derivatives / Q000032:analysis; D024841:Fluoroquinolones; D015363:Quinolones; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D015363:Quinolones; C054745:temafloxacin; D002939:Ciprofloxacin; C047225:difloxacin; C047224:sarafloxacin