[A quantitative ELISA for the measurement of class-specific anti-spermatozoa antibodies].
A class-specific ELISA for the quantitative determination of anti-spermatozoal antibodies is described. The intra-assay variation at the lowest standard concentration for anti-spermatozoal-IgG- and anti-spermatozoal-IgA-specific measurements was 6%. The test possessed an inter-assay variation at the lowest standard concentration in case of the anti-spermatozoal-IgG-determination of 9% and for anti-spermatozoal-IgA-determinations of 8.5%. With this ELISA 5.7% anti-spermatozoal-IgAs and 11.3% anti-spermatozoal-IgGs were identified in sera of a group of infertile females (N = 53). A collective of anti-spermatozoal antibody positive sera, identified by an anti-spermatozoal antibody ELISA measuring total immunoglobulins was subjected to an investigation with the class-specific ELISA. The proportion of anti-spermatozoal-IgAs (54%) was significantly lower than that of anti-spermatozoal-IgAs (78%). Interestingly, no correlation was observed between the parameters IgA and IgG in antispermatozoal positive sera (r = 0.139).
Meinecke
B
B
Firma Seratec GmbH Göttingen.
Stibbe
W
W
vd Kammer
H
H
Scheit
K H
KH
D000906:Antibodies / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000302:isolation & purification; D007247:Infertility, Female / Q000276:immunology*; D008297:Male; D012016:Reference Values; D013094:Spermatozoa / Q000276:immunology*
D000906:Antibodies; D007070:Immunoglobulin A; D007074:Immunoglobulin G
Ein quantitativer ELISA zur Messung von klassenspezifischen Anti-Spermatozoen-Antikörpern.