Release of human serum albumin from poly(lactide-co-glycolide) microspheres.
Human serum albumin (HSA) was encapsulated in a 50:50 copolymer of DL-lactide/glycolide in the form of microspheres. These microspheres were used as a model formulation to study the feasibility of controlling the release of large proteins over a 20- to 30-day period. We show that HSA can be successfully incorporated into microspheres and released intact from these microspheres into various buffer systems at 37 degrees C. A continuous release of the protein could be achieved in physiological buffers at 37 degrees C over a 20- to 30-day period from microspheres with high protein loadings (11.6%). These results demonstrate the potential of poly(DL-lactide-co-glycolide) microspheres for continuous delivery of large proteins.
Hora
M S
MS
Cetus Corporation, Emeryville, California 94608.
Rana
R K
RK
Nunberg
J H
JH
Tice
T R
TR
Gilley
R M
RM
Hudson
M E
ME
D002021:Buffers; D002626:Chemistry, Pharmaceutical; D004058:Diffusion; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D008970:Molecular Weight; D011091:Polyesters / Q000737:chemistry*; D011098:Polyglactin 910 / Q000737:chemistry*; D011489:Protein Denaturation; D012709:Serum Albumin / Q000737:chemistry*
D002021:Buffers; D011091:Polyesters; D012709:Serum Albumin; D011098:Polyglactin 910; C033616:poly(lactide)