Further study on gas phase acid hydrolysis of protein: improvement of recoveries for tryptophan, tyrosine, and methionine.
A novel method for vapor phase acid hydrolysis of protein suitable for quantitative analysis of tryptophan is presented. The hydrolysis is carried out in vapor of a mixture made of 7 M HCl, 10% trifluoroacetic acid, and 20% thioglycolic acid in the presence of indole. Reasonably good recoveries of common amino acids, including tryptophan (above 75%), were achieved.
Yano
H
H
Research Institute for Biosciences, Science University of Tokyo, Chiba.
Aso
K
K
Tsugita
A
A
D006851:Hydrochloric Acid; D006868:Hydrolysis; D008715:Methionine / Q000737:chemistry* / Q000378:metabolism; D009113:Muramidase / Q000737:chemistry; D009211:Myoglobin / Q000737:chemistry; D010206:Papain / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D014269:Trifluoroacetic Acid; D014364:Tryptophan / Q000737:chemistry* / Q000378:metabolism; D014443:Tyrosine / Q000737:chemistry* / Q000378:metabolism; D014835:Volatilization
D009211:Myoglobin; D011506:Proteins; D014443:Tyrosine; D014364:Tryptophan; D008715:Methionine; D014269:Trifluoroacetic Acid; D009113:Muramidase; D010206:Papain; D006851:Hydrochloric Acid