Biological reactivity of Moraxella bovis lipopolysaccharide.
Lipopolysaccharide (LPS) was isolated from Moraxella bovis 118F and ATCC 10900, M ovis ATCC 33078, and M phenylpyruvica ATCC 23333 by hot phenol-water extraction. In silver-stained sodium dodecyl sulfate polyacrylamide electrophoresis gels, M bovis 118F LPS had a smooth profile, whereas the other Moraxella preparations appeared to be rough. The LPS preparations induced pyrogenicity and dermal Shwartzman reactions in rabbits, and induced production of tumor necrosis factor and interleukin-1 in vitro. Induction of tumor necrosis factor appeared to be among the most potent biological activities of M bovis LPS.
Johansen
K A
KA
Veterinary Medical Research Institute, Iowa State University, Ames 50011.
Wannemuehler
M J
MJ
Rosenbusch
R F
RF
D004591:Electrophoresis, Polyacrylamide Gel; D005334:Fever / Q000139:chemically induced; D008070:Lipopolysaccharides / Q000494:pharmacology*; D009016:Moraxella / Q000472:pathogenicity; D012790:Shwartzman Phenomenon; D013045:Species Specificity; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha