Fusarochromanone production by Fusarium isolates.
Sixty two Fusarium isolates representing nine species from many parts of the world were screened for fusarochromanone production. A simplified method for the detection of fusarochromanone in culture filtrates or grain cultures was used. Under UV irradiation (364 nm) the chloroform phase from fusarochromanone-positive culture extracts fluoresced a characteristic bright blue color. Results were confirmed by thin-layer-chromatography comparison with pure fusarochromanone standards. Detection was possible in cultures as young as 1 week old. Biosynthesis of fusarochromanone was rare in Fusarium spp. and was only detected in three isolates of Fusarium equiseti, namely R-4482 (barley [Federal Republic of Germany]), R-6137 (barley [Alaska]), and R-8508 (potato [Denmark]), among all the isolates tested from various geographic sources.
Wu
W D
WD
Department of Plant Pathology, University of Wisconsin, Madison 53706.
Nelson
P E
PE
Cook
M E
ME
Smalley
E B
EB
D000596:Amino Acids / Q000096:biosynthesis*; D002851:Chromatography, High Pressure Liquid; D002867:Chromones / Q000378:metabolism*; D005470:Fluorometry; D005670:Fusarium / Q000145:classification / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D008828:Microbiological Techniques; D013045:Species Specificity
D000596:Amino Acids; D002867:Chromones; C054523:fusarochromanone