Synthesis of a water-soluble protein cross-linking reagent.
2,4-Difluoro-5-nitrobenzenesulfonic acid has been synthesized by the sulfonation of 2,4-difluoronitrobenzene, and precipitated with KCl as the potassium sulfonate. The structure was confirmed by chemical and spectroscopic methods (IR, 1H-NMR, 13C-NMR, 19F-NMR, UV, MS and ultimate organic analysis). Lysozyme was cross-linked with the potassium sulfonate and with 1,5-difluoro-2,4-dinitrobenzene. The products were analysed by SDS-PAGE and compared. The cross-linking conditions were optimized.
Kremer
U M
UM
Institut für Physikalische Chemie, Abteilung für Biopolymere, RWTH Aachen.
D000818:Animals; D001557:Benzenesulfonates / Q000138:chemical synthesis; D002645:Chickens; D003432:Cross-Linking Reagents / Q000138:chemical synthesis*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D009113:Muramidase / Q000378:metabolism; D009574:Nitro Compounds / Q000138:chemical synthesis; D012996:Solutions; D013055:Spectrophotometry, Infrared; D014867:Water
D001557:Benzenesulfonates; D003432:Cross-Linking Reagents; D009574:Nitro Compounds; D012996:Solutions; D014867:Water; C067691:2,4-difluoro-5-nitrobenzene sulfonic acid; D009113:Muramidase