[Highly selective affinity labeling of DNA-dependent RNA-polymerase II from human placenta].
RNA polymerase II from human placenta was affinity labelled in crude preparation using two-step technique, which includes treatment of the enzyme with an aldehyde-containing reactive analogue of ATP, ADP or AMP in the presence of poly[d(A-T)] followed (after borohydride reduction) by the elongation of the attached label with [alpha-32P]UTP. A polypeptide of the molecular mass ca. 140 kDa proved to be the labelling target. No labelling was observed in the absence of poly[d(A-T)] or the reagent or in the presence of alpha-amanitin. All the results suggest the attachment of the affinity reagents to the second-largest subunit of the human RNA polymerase II, which therefore takes part in the initiation substrate's binding.
Maksimova
T G
TG
Mustaev
A A
AA
Zaĭchikov
E F
EF
Polukhin
A V
AV
Raĭt
V K
VK
D000345:Affinity Labels; D001665:Binding Sites; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D010920:Placenta / Q000201:enzymology*; D012319:RNA Polymerase II / Q000737:chemistry*
D000345:Affinity Labels; D012319:RNA Polymerase II
Vysokoselektivnoe affinnoe mechenie DNK-zavisimoĭ RNK-polimerazy II iz platsenty cheloveka.