Genetic recombination in human melanoma and astrocytoma cell lines involves oncogenes and growth factor genes.
We describe here the chromosomal distribution of sister chromatid exchanges (SCEs) in four human tumor cell lines (two melanomas and two astrocytomas), and have mapped the sister chromatid recombination (SCR) sites. A higher incidence of SCR sites than expected on the basis of chromosome length occurred in chromosomes 2, 4, 5 and 15 in both the RPMI 5966 and MEL57 melanoma cell lines, and in chromosomes 1, 5, 13 and 15 of the IJKt and GUVW astrocytoma cell lines. A majority of the recombination sites occurred close to chromosomal fragile sites. A third of these occurred at the same bands as fragile sites. The recombination sites involved the N-ras and the epidermal growth factor gene in the melanomas. In the astrocytomas, the N-ras, Rb and c-mos genes appeared to be involved in the recombination events. The beta 2-microglobulin gene was involved in both astrocytomas and one melanoma. The erbB2 was involved in SCR only in the RPMI melanoma.
Lakshmi
M S
MS
Division of Oncology, University of Newcastle upon Tyne, Medical School, U.K.
Sherbet
G V
GV
D001254:Astrocytoma / Q000235:genetics*; D043283:Chromosome Fragile Sites; D002873:Chromosome Fragility; D002874:Chromosome Mapping; D002894:Chromosomes, Human, Pair 4; D005796:Genes; D006133:Growth Substances / Q000235:genetics*; D006801:Humans; D008545:Melanoma / Q000235:genetics*; D009857:Oncogenes; D011995:Recombination, Genetic; D012854:Sister Chromatid Exchange; D014407:Tumor Cells, Cultured / Q000502:physiology
D006133:Growth Substances