Effect of di(2-ethylhexyl)phthalate on enzyme activity levels in liver and serum of rats.
In order to identify non-invasive, biochemical indicators of di(2-ethylhexyl)phthalate (DEHP) exposure, we have compared the effects in blood serum with biochemical effects in liver in rats fed a diet containing 0, 0.25, 0.75 and 2% DEHP for 2 weeks. After 3 days of treatment serum arylesterase activity levels and serum triglycerides were decreased to 60% and 20% of control values, respectively. After a 2-week treatment with DEHP the effects were generally stronger. Compared to a control group, serum arylesterase activity levels, serum triglycerides and serum cholesterol were decreased to 40%, 20% and 50%, respectively. Serum cholinesterase activity levels and serum albumin concentrations were increased by the DEHP treatment to 290% and 135% of control values, respectively. In the livers a hepatomegaly, an induction of cytochrome P-450 IVA1 and induction of the activity of palmitoyl-CoA oxidase and carnitine acetyl-CoA transferase was found to be 180%, 1080%, 1300% and 1700% of control values, respectively. The liver is a more sensitive target for DEHP exposure compared to the biochemical effects in serum, but determination of the serum parameters can be used to determine early biological effects of exposure to DEHP.
Dirven
H A
HA
Department of Toxicology, University of Nijmegen, The Netherlands.
van den Broek
P H
PH
Jongeneelen
F J
FJ
D042962:Acyl-CoA Oxidase; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002265:Carboxylic Ester Hydrolases / Q000097:blood*; D002332:Carnitine O-Acetyltransferase / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D002802:Cholinesterases / Q000097:blood*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004051:Diethylhexyl Phthalate / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010088:Oxidoreductases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000097:blood
D014280:Triglycerides; D003577:Cytochrome P-450 Enzyme System; D002784:Cholesterol; D004051:Diethylhexyl Phthalate; D010088:Oxidoreductases; D042962:Acyl-CoA Oxidase; D002332:Carnitine O-Acetyltransferase; D002265:Carboxylic Ester Hydrolases; C019734:arylesterase; D002802:Cholinesterases