title: Isolation of the perisinusoidal vitamin A-storing cells from rat liver on Ca2(+)-immobilized glass surfaces.
abstract: Calcium was immobilized on glass slides employing covalently coupled iminodiacetic acid. The calcium-coated glass surfaces were then used for purification and culturing of perisinusoidal vitamin A-storing cells from rat liver. The cells were isolated in a yield of 0.9 x 10(6) cells/g rat liver without cross-contamination by other hepatic cell types. The cells were characterized by morphology, vitamin A fluorescence, and immunohistochemistry to detect expression of cellular retinol-binding protein.
authors.affiliation: Institute of Biochemistry, University of Uppsala, Sweden.
authors.forename: T
authors.initials: T
authors.lastname: Mantovaara
authors.forename: H
authors.initials: H
authors.lastname: Nordlinder
authors.forename: H
authors.initials: H
authors.lastname: Pertoft
mesh_terms: D000818:Animals; D002118:Calcium; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D005898:Glass; D007150:Immunohistochemistry; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014801:Vitamin A / Q000378:metabolism*
chemical_list: D014801:Vitamin A; D002118:Calcium