A novel peptide which stimulates adenylate cyclase: molecular cloning and characterization of the ovine and human cDNAs.
A novel neuropeptide which remarkably stimulates adenylate cyclase in rat anterior pituitary cell cultures has been recently isolated from ovine hypothalami by A. Arimura and his collaborators (Biochem.Biophys.Res.Commun.164, 567-574(1989)). This peptide was designated as PACAP38(Pituitary Adenylate Cyclase Activating Polypeptide with 38 residues). In an attempt to investigate physiological implications of PACAP38, we have succeeded in cloning the cDNAs encoding the precursor of PACAP38 from ovine hypothalamus and human testis. An ovine cDNA encodes a protein of 176 amino acids in which PACAP38 is proceeded by a putative signal peptide and a "pro"-region (107 amino acids), and followed by a Gly-Arg-Arg sequence for proteolytic processing and amidation. Deduced amino-acid sequence of human PACAP38 was completely identical to that of the ovine isolated peptide. Cloning of PACAP38 cDNAs confirms the expression of the corresponding mRNAs and the presence of this neuropeptide in ovine hypothalamus and also in human testis.
Kimura
C
C
Tsukuba Research Laboratories, Takeda Chemical Industries, Ltd., Ibaraki, Japan.
Ohkubo
S
S
Ogi
K
K
Hosoya
M
M
Itoh
Y
Y
Onda
H
H
Miyata
A
A
Jiang
L
L
Dahl
R R
RR
Stibbs
H H
HH
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004789:Enzyme Activation; D006801:Humans; D007031:Hypothalamus / Q000378:metabolism*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics* / Q000502:physiology; D015345:Oligonucleotide Probes; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D011487:Protein Conformation; D011498:Protein Precursors / Q000235:genetics; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep
C495401:ADCYAP1 protein, human; D009479:Neuropeptides; D015345:Oligonucleotide Probes; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D011498:Protein Precursors; D004247:DNA; D000262:Adenylyl Cyclases