Capillary zone electrophoresis-mass spectrometry using a coaxial continuous-flow fast atom bombardment interface.
Mixtures of peptides have been analyzed by capillary zone electrophoresis in conjunction with mass spectrometry (MS) using an on-line coaxial continuous-flow fast atom bombardment interface. MS and MS-MS spectra have been acquired in electrophoretic real time from femtomole levels of the peptides, while maintaining separation efficiencies in excess of 100,000 theoretical plates.
Moseley
M A
MA
Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.
Deterding
L J
LJ
Tomer
K B
KB
Jorgenson
J W
JW
D000596:Amino Acids / Q000032:analysis; D000700:Analgesics / Q000032:analysis; D002630:Chemotactic Factors / Q000032:analysis; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D004723:Endorphins / Q000032:analysis; D015394:Molecular Structure; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000031:analogs & derivatives / Q000032:analysis; D009479:Neuropeptides / Q000032:analysis; D009842:Oligopeptides / Q000032:analysis; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000295:instrumentation / Q000379:methods*
D000596:Amino Acids; D000700:Analgesics; D002630:Chemotactic Factors; D004723:Endorphins; D009479:Neuropeptides; D009842:Oligopeptides; C011536:proctolin; D009240:N-Formylmethionine Leucyl-Phenylalanine; C042205:methionyl-leucyl-phenylalanine; C028889:morphiceptin