High molecular weight forms of basic fibroblast growth factor recognized by a new anti-bFGF antibody.
An antibody against basic fibroblasts growth factor (bFGF) was raised using purified bovine pituitary bFGF. Western blot analysis revealed immunoreactive bands at 18, 24, 30-33 and 46 kDa in immunoaffinity purified extracts of pituitary and adrenal gland using this antibody. A similar staining pattern was obtained with ovary extracts with the exception of the missing 18 kDa band. A second anti-bFGF antibody raised against a synthetic peptide comprising the 24 N-terminal amino acids of bFGF reacted with the 18 kDa and the 46 kDa band of immunoaffinity purified ovary and adrenal gland extracts.
Grothe
C
C
Department of Anatomy and Cell Biology, Philipps-University of Marburg, FRG.
Zachmann
K
K
Unsicker
K
K
Westermann
R
R
D000311:Adrenal Glands / Q000032:analysis / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000915:Antibody Affinity; D000941:Antigens / Q000032:analysis; D015153:Blotting, Western; D002417:Cattle; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005346:Fibroblast Growth Factors / Q000032:analysis* / Q000276:immunology; D007106:Immune Sera / Q000096:biosynthesis; D008970:Molecular Weight; D010053:Ovary / Q000032:analysis / Q000276:immunology; D010902:Pituitary Gland / Q000032:analysis* / Q000276:immunology; D014020:Tissue Extracts / Q000032:analysis
D000906:Antibodies; D000941:Antigens; D007106:Immune Sera; D014020:Tissue Extracts; D005346:Fibroblast Growth Factors