title: Androgen receptor heterogeneity and phosphorylation in human LNCaP cells.
abstract: Androgen receptor heterogeneity and phosphorylation were studied in the human LNCaP cell line. Fluorography after photoaffinity labeling as well as immunoblotting with a specific polyclonal antibody revealed that the human androgen receptor migrated as a closely spaced 110 kD doublet on SDS-polyacrylamide gels. A time-dependent change in the ratio between the two isoforms was not observed after R1881 treatment of intact cells. In nuclear extracts of LNCaP cells that were incubated with [32P]orthophosphate in the presence of 10 nM R1881, a 110 kD phosphorylated protein was demonstrated after immunopurification using a monoclonal antibody against the human androgen receptor. Only a very small amount of this phosphoprotein was detected in the nuclear fraction from cells not treated with R1881. These results indicate that the human androgen receptor in LNCaP cells can be phosphorylated.
authors.affiliation: Department of Biochemistry II, Erasmus University Rotterdam, The Netherlands.
authors.forename: J H
authors.initials: JH
authors.lastname: van Laar
authors.forename: J
authors.initials: J
authors.lastname: Bolt-de Vries
authors.forename: N D
authors.initials: ND
authors.lastname: Zegers
authors.forename: J
authors.initials: J
authors.lastname: Trapman
authors.forename: A O
authors.initials: AO
authors.lastname: Brinkmann
mesh_terms: D001345:Autoradiography; D015153:Blotting, Western; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D006801:Humans; D008223:Lymphoma; D008297:Male; D008970:Molecular Weight; D010710:Phosphates / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D011471:Prostatic Neoplasms; D011944:Receptors, Androgen / Q000302:isolation & purification / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000378:metabolism*
chemical_list: D010710:Phosphates; D010761:Phosphorus Radioisotopes; D011944:Receptors, Androgen