title: Postburn suppression of murine lymphocyte and neutrophil functions is not reversed by prostaglandin blockade.
abstract: Certain arachidonic acid metabolites, including prostaglandins (PGs) E1 and E2, have been shown to exert marked immunosuppressive effects on T-cell and macrophage functions. Cyclooxygenase blockade with indomethacin or ibuprofen may ameloriate these effects. In the current study we measured lymphocyte proliferation by thymidine incorporation, the presence of T-cell activation antigens with monoclonal antibodies and two-color flow cytometry, and neutrophil (PMN) oxidative burst using a fluorescent marker, in control mice and in burned mice treated with indomethacin for 10 days after injury. One-half of the cell cultures were treated with indomethacin in vitro to ensure its continued presence during stimulation. Separate groups of mice were fed a fish oil-based diet which leads to the production of PGE3 rather than PGE2, versus standard mouse chow, a soy-bean oil-based diet which leads to PGE2 production. Lymphocyte proliferation, expression of T-cell activation antigens, and PMN oxidative burst remained depressed in burned mice treated with indomethacin in vivo (plus in vitro) and in those which received the fish oil-based diet, compared to control. Blockade of PG synthesis after murine burn injury by cyclooxygenase inhibition or alterations in the diet failed to restore T-lymphocyte activation or proliferation or to improve PMN oxidative burst. These data suggest that PGE2 alone does not explain the immunosuppression noted after burn injury.
authors.affiliation: Department of Surgery, University of California San Diego Medical Center 92103.
authors.forename: M A
authors.initials: MA
authors.lastname: Gadd
authors.forename: J F
authors.initials: JF
authors.lastname: Hansbrough
mesh_terms: D000818:Animals; D002056:Burns / Q000378:metabolism / Q000503:physiopathology*; D002455:Cell Division / Q000187:drug effects; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology; D015232:Dinoprostone / Q000494:pharmacology; D005260:Female; D005395:Fish Oils / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000502:physiology*; D051379:Mice; D008815:Mice, Inbred Strains; D009504:Neutrophils / Q000378:metabolism / Q000502:physiology*; D010084:Oxidation-Reduction / Q000187:drug effects; D011448:Prostaglandin Antagonists / Q000494:pharmacology*; D012016:Reference Values; D013601:T-Lymphocytes
chemical_list: D004042:Dietary Fats, Unsaturated; D005395:Fish Oils; D011448:Prostaglandin Antagonists; D015232:Dinoprostone; D007213:Indomethacin