Dry storage of liposome-encapsulated hemoglobin: a blood substitute.
We have previously demonstrated the stabilization of liposome-encapsulated hemoglobin (LEH) by lyophilization (Cryobiology 25, 277-284, 1988). In the present report, we examine the structural and functional recovery of LEH after 3 months in the dry state. We have investigated the incorporation of the protective carbohydrate trehalose in the production and preservation of lyophilized LEH. Vesicle size, retention of entrapped hemoglobin, oxygen-carrying capacity, and percentage methemoglobin were measured as a function of time stored in the dry state under vacuum at room temperature. The results indicate that 150-300 mM trehalose maintains LEH dry preparations with little change in their size or functional characteristics after 3 months in the dry state. These results are compared to those of LEH that has been stored hydrated at 4 degrees C for the same time period.
Rudolph
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Bio/Molecular Engineering Branch, Naval Research Laboratory, Washington, DC 20375-5000.
Cliff
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D000818:Animals; D001802:Blood Substitutes; D002417:Cattle; D004356:Drug Storage; D005612:Freeze Drying; D006454:Hemoglobins; D008081:Liposomes; D010316:Particle Size; D013997:Time Factors; D014199:Trehalose
D001802:Blood Substitutes; D006454:Hemoglobins; D008081:Liposomes; D014199:Trehalose