UVB radiation and human monocyte accessory function: differential effects on pre-mitotic events in T-cell activation.
Purified T lymphocytes fail to proliferate in response to antigenic and mitogenic stimuli when cultured in the presence of accessory cells that have been exposed in vitro to sublethal doses of UVB radiation. Because proliferation represents a final stage in the T-cell activation process, the present study was conducted to determine whether T cells were able to progress through any of the pre-mitotic stages when UVB-irradiated monocytes were used as model accessory cells. In these experiments, monoclonal anti-CD3 antibodies were employed as the mitogenic stimulus. Culture of T cells with UVB-irradiated monocytes did allow the T cells to undergo an increase in intracellular free calcium, which is one of the first steps in the activation sequence. The T cells expressed interleukin-2 receptors, although at a reduced level. However, T cells failed to produce interleukin-2 above background levels when they were placed in culture with monocytes exposed to UVB doses as low as 50 J/m2. Incubation of T cells with UVB-irradiated monocytes did not affect the subsequent capacity of T cells to proliferate, since they developed a normal proliferative response in secondary culture when restimulated with anti-CD3 antibodies and unirradiated monocytes. These studies indicate that T lymphocytes become partially activated when cultured with UVB-irradiated monocytes and mitogenic anti-CD3 monoclonal antibodies. In addition, they suggest that interleukin-2 production is the T-cell activation step most sensitive to inhibition when UVB-irradiated monocytes are employed as accessory cells.
Krutmann
J K
JK
Department of Dermatology, Case Western Reserve University, Cleveland, Ohio.
Kammer
G M
GM
Toossi
Z
Z
Waller
R L
RL
Ellner
J J
JJ
Elmets
C A
CA
D015703:Antigens, CD / Q000494:pharmacology; D002118:Calcium / Q000032:analysis; D003593:Cytoplasm / Q000032:analysis; D006801:Humans; D008213:Lymphocyte Activation / Q000528:radiation effects*; D008938:Mitosis; D009000:Monocytes / Q000528:radiation effects*; D015375:Receptors, Interleukin-2 / Q000502:physiology; D013601:T-Lymphocytes / Q000276:immunology*; D014466:Ultraviolet Rays
D015703:Antigens, CD; D015375:Receptors, Interleukin-2; D002118:Calcium