title: Heat-induced cytotoxicity in H2O2-resistant Chinese hamster fibroblasts.
abstract: Hydrogen-peroxide-resistant Chinese hamster fibroblasts, derived from the HA-1 cell line, were isolated following continuous culturing in the presence of progressively increasing concentrations of hydrogen peroxide. The hydrogen-peroxide-resistant phenotype has been stable for over 360 days following removal from H2O2 stress. These H2O2-resistant cell lines demonstrate increased resistance to hyperthermic cell killing mediated by continuous heating at 43 degrees C but not 45 degrees C. The relationship between mammalian cellular adaptation to oxidative stress mediated by H2O2 and resistance to 43 degrees C hyperthermia is discussed.
authors.affiliation: Radiation Oncology Research Laboratory, University of California, San Francisco 94143.
authors.forename: D R
authors.initials: DR
authors.lastname: Spitz
authors.forename: G C
authors.initials: GC
authors.lastname: Li
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002999:Clone Cells; D006224:Cricetinae; D004171:Diploidy; D004351:Drug Resistance; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D011003:Ploidies
chemical_list: D006861:Hydrogen Peroxide