[Spermatocyte antibodies in serum and follicular fluid of IVF and GIFT patients].
With a new SPAK-ELISA test system (Seratec GmbH) we determined sperm antibodies in sera of 60 IVF, 11 GIFT patients and their husbands. We evaluated sperm antibodies in sera of 12 IVF (20.0%), 3 GIFT (27%) and 3 males with normozoospermia parameters (4.2%). Compared to seronegative patients either the number of oocytes and their quality as the fertilization- and embryo transfer rate during the IVF/GIFT procedure were not affected. No intact pregnancy occurred in cases of sperm antibodies in sera. In 16.6% of all seropositive IVF patients the detection of sperm antibodies in the follicular fluids correspond to sera. We recommend to remove autologous serum for the oocyte culture in cases of sperm antibodies in serum of IVF patients.
Baguhl
F
F
Abteilung für Reproduktionsmedizin, Bereichs Medizin, Universität Rostock.
Bernt
W D
WD
Kunkel
S
S
Sudik
R
R
D000906:Antibodies / Q000302:isolation & purification*; D001826:Body Fluids / Q000276:immunology; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D015181:Gamete Intrafallopian Transfer; D006801:Humans; D008297:Male; D006080:Ovarian Follicle / Q000276:immunology; D013090:Spermatocytes / Q000276:immunology*
D000906:Antibodies
Spermatozyten-Antikörper im Serum und in der Follikelflüssigkeit von IVF- und GIFT-Patienten.