Complete coding sequence of a human B-raf cDNA and detection of B-raf protein kinase with isozyme specific antibodies.
A 2.2 kb cDNA clone which presumably includes the complete human B-raf coding sequence was isolated from a human testes cDNA library. Sequence analysis showed the presence of all three conserved regions CR1, CR2 and CR3 previously identified in raf protein kinases. The cDNA was expressed in E. coli yielding a B-raf fusion protein which reacts with antibodies specific for B-raf raised against the C-terminal peptide as well as with monoclonal antibodies which map into the kinase domain.
Sithanandam
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Laboratory of Viral Carcinogenesis, National Cancer Institute, Frederick Cancer Research and Development Center, Maryland 21702-1201.
Kolch
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W
Duh
F M
FM
Rapp
U R
UR
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D004247:DNA / Q000235:genetics*; D004926:Escherichia coli; D006801:Humans; D015151:Immunoblotting; D007527:Isoenzymes / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D011494:Protein Kinases / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D019908:Proto-Oncogene Proteins c-raf
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D011518:Proto-Oncogene Proteins; D004247:DNA; D011494:Protein Kinases; D019908:Proto-Oncogene Proteins c-raf