title: Evaluation of LAK-mediated tumor cell killing in a plasma clot clonogenic assay.
abstract: An assay based on the inhibition of the cloning capacity in a plasma clot semisolid medium assay has been used to test the sensitivity of the Raji cell line to lymphokine-activated killer (LAK) cells. This method overcomes some limitations intrinsic to the widely employed 51Cr release assay and always shows a higher degree of sensitivity. No inhibition of colony growth was found when the effector cells were plated without prior pre-incubation with interleukin 2 or with the addition of the medium derived from the LAK cells. Though more time-consuming than the classic 51Cr release assay, this technique does not require radioactive material. This test may be suitable for a more precise evaluation of LAK activity and for the study of the mechanisms involved in cell killing.
authors.affiliation: Divisione di Ematologia, Ospedale S. Carlo, Bologna, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Dinota
authors.forename: P L
authors.initials: PL
authors.lastname: Tazzari
authors.forename: A
authors.initials: A
authors.lastname: Bontadini
authors.forename: D
authors.initials: D
authors.lastname: Raspadori
authors.forename: C
authors.initials: C
authors.lastname: Tassi
authors.forename: L
authors.initials: L
authors.lastname: Zucchini
authors.forename: M
authors.initials: M
authors.lastname: Pizzuti
authors.forename: F
authors.initials: F
authors.lastname: Ricciuti
authors.forename: M
authors.initials: M
authors.lastname: Gobbi
mesh_terms: D002051:Burkitt Lymphoma / Q000473:pathology; D003601:Cytotoxicity Tests, Immunologic; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D014407:Tumor Cells, Cultured; D014410:Tumor Stem Cell Assay
chemical_list: D007376:Interleukin-2