title: Sequential morphological changes in chicken erythrocytes after in vivo and in vitro exposure to phenylhydrazine-hydrochloride.
abstract: In acute haemolytic anaemia of chickens, induced with the oxidant chemical phenylhydrazine-hydrochloride, maximum degenerative changes in the in vivo exposed erythrocytes occurred on day 3 after injection. Microspherocytic transformation, dumb-bell shaped red blood cells and foamy squashed nuclei predominated. Heinz body formation was the cytoplasmic hallmark of the haemolytic anaemia. Marked reticulocytosis on day 5 indicated spontaneous regeneration. In vitro exposure to the chemical agent evoked similar morphological aberrations/Heinz bodies, microspherocytic transformation and nuclear degeneration. These were induced much earlier, presumably because of the absence of a protective internal milieu. Nevertheless, a basic similarity in morphology was evident. The in vitro test system might be suitable for screening oxidant chemicals and drugs.
authors.affiliation: Department of Veterinary Medicine, College of Veterinary Science, Jabalpur, India.
authors.forename: K
authors.initials: K
authors.lastname: Datta
authors.forename: J L
authors.initials: JL
authors.lastname: Soni
authors.forename: I C
authors.initials: IC
authors.lastname: Datta
mesh_terms: D000743:Anemia, Hemolytic / Q000097:blood / Q000662:veterinary*; D000818:Animals; D002645:Chickens; D004903:Erythrocyte Aggregation / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D010659:Phenylhydrazines / Q000494:pharmacology*; D011201:Poultry Diseases / Q000097:blood*
chemical_list: D010659:Phenylhydrazines