Molecular forms of butyrylcholinesterase in rat brain microvessels.
Molecular forms of butyrylcholinesterase (BuChE) were studied in microvessels isolated from rat brain, using sedimentation analysis in sucrose gradients. Three forms, G1, G2 and G4, were found with sedimentation coefficients close to 3S, 6S and 9S, respectively. The relative proportion of the 3 forms was 19% for the monomer, 33% for the dimer and 46% for the tetramer. This sedimentation pattern of BuChE forms appears to be characteristic of cerebral microvessels and may represent distinct functional features of the blood-brain barrier.
Catalán
R E
RE
Departamento de Biología Molecular, Universidad Autónoma de Madrid, Spain.
Martínez
A M
AM
Aragonés
M D
MD
Hernández
F
F
D000818:Animals; D002091:Butyrylcholinesterase / Q000032:analysis*; D002196:Capillaries / Q000201:enzymology*; D002499:Centrifugation, Density Gradient; D002560:Cerebrovascular Circulation; D046911:Macromolecular Substances; D008297:Male; D008970:Molecular Weight; D009131:Muscle, Smooth, Vascular / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains
D046911:Macromolecular Substances; D002091:Butyrylcholinesterase