Is intestinal villus phospholipase A2/lysophospholipase bound pancreatic carboxylester lipase?
Similarities in substrate specificity, localization and molecular weight between villus membrane phospholipase A2/lysophospholipase and carboxylester lipase of pancreatic origin suggested their possible identity. To test this, a preparation of the phospholipase A2/lysophospholipase released from brush border vesicles by papain was compared to authentic, pancreatic carboxylester lipase. Susceptibility of both activities to the inhibitor, diisopropylfluorophosphate, was consistent with their identity, but inconclusive. It also indicated that two populations of phospholipase A2 species may be present in the papain-released preparation. However, comparison of binding of the activities to Sepharose-coupled, anti-carboxylester-lipase IgG indicates that they are immunologically distinct.
Mizuno
N K
NK
Hormel Institute, University of Minnesota, Austin 55912.
Brockman
H L
HL
D000818:Animals; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D007074:Immunoglobulin G / Q000276:immunology; D007413:Intestinal Mucosa / Q000201:enzymology* / Q000648:ultrastructure; D007531:Isoflurophate / Q000494:pharmacology; D008245:Lysophospholipase / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D008297:Male; D008871:Microvilli / Q000201:enzymology; D010179:Pancreas / Q000201:enzymology*; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D054467:Phospholipases A2; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains
D007074:Immunoglobulin G; D007531:Isoflurophate; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; D010741:Phospholipases A; D054467:Phospholipases A2; D008245:Lysophospholipase