title: Fluoroaluminate mimics agonist application in single rat hepatocytes.
abstract: Single rat hepatocytes microinjected with the photoprotein aequorin were stimulated with glycogenolytic agonists or low concentrations of fluoroaluminate. Both protocols resulted in the generation of oscillations in cytosolic free Ca2+ levels from a resting value of approx. 200 nM and peaking at over 600 nM. However, oscillations induced by receptor-dependent agonists were more regular in both frequency and time course than those induced by direct activation of G-proteins. The role of G-proteins in the generation of repetitive free Ca2+ oscillations is discussed.
authors.affiliation: Department of Human Anatomy and Cell Biology, University of Liverpool, U.K.
authors.forename: N M
authors.initials: NM
authors.lastname: Woods
authors.forename: C J
authors.initials: CJ
authors.lastname: Dixon
authors.forename: K S
authors.initials: KS
authors.lastname: Cuthbertson
authors.forename: P H
authors.initials: PH
authors.lastname: Cobbold
mesh_terms: D000331:Aequorin; D000535:Aluminum / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D005461:Fluorine / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D010656:Phenylephrine / Q000494:pharmacology*; D051381:Rats
chemical_list: D010656:Phenylephrine; C053629:fluoroaluminum; D005461:Fluorine; D000331:Aequorin; D000535:Aluminum; D002118:Calcium