Detection of genomic alterations in carcinogen-induced mouse liver tumors by DNA fingerprint analysis.
DNA fingerprint analysis was used to study structural abnormalities in the genome of mouse liver tumor cells. Liver tumors were induced in three strains of mice, namely C57BL/6J, C3H/He and B6C3F1, by a single injection of 20 micrograms/g body wt. diethylnitrosamine on day 15 after birth. DNA from liver tumors was digested with Hinfl restriction enzyme and hybridized on Southern blots with wild-type bacteriophage M13 DNA as probe. The resulting fingerprints of tumor DNA were compared with those of DNA from normal liver tissue. Genomic aberrations were detected in two out of 68 tumors analyzed, one stemming from a C57BL/6J and the other from a C3H/He mouse.
Müller
O
O
German Cancer Research Center, Institute of Experimental Pathology, Heidelberg.
Alonso
A
A
Buchmann
A
A
Bauer-Hofmann
R
R
Bock
K W
KW
Schwarz
M
M
D000818:Animals; D015139:Blotting, Southern; D016172:DNA Fingerprinting; D004273:DNA, Neoplasm / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D004052:Diethylnitrosamine; D008113:Liver Neoplasms / Q000235:genetics*; D051379:Mice; D008815:Mice, Inbred Strains; D009693:Nucleic Acid Hybridization
D004273:DNA, Neoplasm; D004279:DNA, Viral; D004052:Diethylnitrosamine; D015252:Deoxyribonucleases, Type II Site-Specific; C032229:GANTC-specific type II deoxyribonucleases