Short chain phospholipids in membrane protein crystallization: a 31P-NMR study of colloidal properties of dihexanoyl phosphatidylcholine.
The colloidal features of short chain phospholipids can be deduced from 31P-NMR analysis by comparison with available data on phospholipid aqueous dispersion. In this study with dihexanoyl phosphatidylcholine, detergent phase separation was obtained by temperature shift and by addition of the precipitating agent polyethylene glycol. The 31P-NMR spectra indicate that the detergent micelles fuse to enter the hexagonal HII and lamellar phases. Consequences for the crystallization of membrane proteins are discussed.
Eisele
J L
JL
Institut Pasteur, Unite d'Immunologie Structurale, Paris, France.
Neumann
J M
JM
Chachaty
C
C
D011232:Chemical Precipitation; D003102:Colloids; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008565:Membrane Proteins / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D010758:Phosphorus; D013696:Temperature; D014961:X-Ray Diffraction
D003102:Colloids; D008565:Membrane Proteins; D010713:Phosphatidylcholines; D010743:Phospholipids; D010758:Phosphorus; C032166:1,2-hexanoylphosphatidylcholine