title: [Isolation of human thrombin by affinity chromatography with silicate to be used in the preparation of biological glue].
abstract: At present the preparations of fibrin glue used a thrombin from equine or bovine origin. In order to remove the problems of antigenicity we developed a method of purification from acidified plasma. We used one affinity chromatography on benzamidine-Spherodex and compared three methods of elution: non specific (sodium chloride gradient) and biospecific competitors (arginin methylester or benzamidin). The yield is evaluated between 64 and 84% and the purification factor close to 160. The obtained thrombin is better than animal thrombin in the preparations of fibrin glue.
authors.affiliation: Centre de Transfusion Sanguine des Armées Jean-Julliard, Clamart.
authors.forename: M
authors.initials: M
authors.lastname: Allary
authors.forename: E
authors.initials: E
authors.lastname: Boschetti
authors.forename: J L
authors.initials: JL
authors.lastname: Lorne
mesh_terms: D001550:Benzamidines; D002846:Chromatography, Affinity / Q000379:methods; D015718:Fibrin Tissue Adhesive; D006801:Humans; D012965:Sodium Chloride; D013917:Thrombin / Q000302:isolation & purification*
chemical_list: D001550:Benzamidines; D015718:Fibrin Tissue Adhesive; D012965:Sodium Chloride; D013917:Thrombin
vernacular_title: Isolement par chromatographie d'affinité, sur support de silice, de la thrombine humaine en vue de son utilisation dans les préparations de colle biologique.