title: Vitamin E uptake by human intestinal cells during lipolysis in vitro.
abstract: Vitamin E uptake by Caco-2 cells, a human intestinal cell line, was studied by incubating the cells with alpha-tocopherol/triglyceride emulsions with or without bile activated lipase or lipoprotein lipase. During a 1-h incubation, vitamin E was transferred to Caco-2 cells only in the presence of triglyceride hydrolysis by bile activated lipase and not by lipoprotein lipase. Incubation with either lipase resulted in hydrolysis of approximately 20% of the medium [3H]-triolein to free fatty acids and a 3-5-fold increase in cellular radioactivity. In the absence of lipases but the presence of taurocholate, addition of oleic acid in an amount equal to the molar concentration of triglyceride (5.7 mM) to triglyceride emulsions containing either alpha-tocopherol or cholesteryl ester resulted in an increase in cellular [3H]-triglyceride and alpha-tocopherol or cholesteryl ester. We suggest that the absorption of hydrophobic molecules such as vitamin E may occur in the presence of bile and amphipathic lipids via the uptake of micellar neutral lipids by the intestine.
authors.affiliation: Department of Medicine, New York University School of Medicine, New York.
authors.forename: M G
authors.initials: MG
authors.lastname: Traber
authors.forename: I
authors.initials: I
authors.lastname: Goldberg
authors.forename: E
authors.initials: E
authors.lastname: Davidson
authors.forename: N
authors.initials: N
authors.lastname: Lagmay
authors.forename: H J
authors.initials: HJ
authors.lastname: Kayden
mesh_terms: D002460:Cell Line; D002478:Cells, Cultured; D004063:Digestion / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D007408:Intestinal Absorption / Q000502:physiology*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000166:cytology; D008049:Lipase / Q000502:physiology*; D008066:Lipolysis / Q000502:physiology*; D008071:Lipoprotein Lipase / Q000502:physiology; D002787:Sterol Esterase; D014280:Triglycerides / Q000493:pharmacokinetics; D014810:Vitamin E / Q000493:pharmacokinetics*
chemical_list: D014280:Triglycerides; D014810:Vitamin E; C054784:bile salt-stimulated lipase; D002787:Sterol Esterase; D008049:Lipase; D008071:Lipoprotein Lipase