Use of fluorescent in situ hybridisation to confirm trisomy of chromosome region 1q32-qter as the sole karyotypic defect in a colon cancer cell line.
The sole chromosome defect in a colon cancer cell line derived from a patient with inherited nonpolyposis colorectal cancer was karyotypically designated as 46,XY,-13,+der(13)t(1;13)(q32.1;p11) on the basis of banding homology. We have obtained molecular confirmation that the additional chromosome material is derived from chromosome region 1q32-qter by the use of a highly specific fluorescent in situ hybridisation technique on G-banded chromosomes and also by Southern hybridisation.
Griffin
D K
DK
Department of Genetics and Biometry, University College London, England.
Leigh
S E
SE
Delhanty
J D
JD
D015139:Blotting, Southern; D002878:Chromosomes, Human, Pair 1; D003110:Colonic Neoplasms / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics*; D006801:Humans; D007621:Karyotyping; D008856:Microscopy, Fluorescence; D009693:Nucleic Acid Hybridization; D014314:Trisomy; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm