Rapid hyperthermic cell sensitivity test measured by RNA synthesis using contact-sensitive plates of confluent Balb/c 3T3 cell monolayers.
We have developed a hyperthermic sensitivity test for human neoplastic cells using contact-sensitive confluent monolayers of BALB/c 3T3 cells designated as 'contact-sensitive plates (CSP)', which almost completely inhibited the growth of normal cells without influencing the growth of the human neoplastic cells. On 'CSP', the relative [3H]uridine incorporation into RNA of the hyperthermic cultures showed an excellent correlation with the relative clonogenic efficiency of the treated cultures. Since 'CSP' allows for only contact-insensitive tumour cells to proliferate, our assay will predict the hyperthermic sensitivity of only the neoplastic cells. Moreover, even 2 x 10(3) cells derived from clinical tumour tissues can be assayed within only 3 days. This assay system is potentially used as a rapid in vitro hyperthermic sensitivity test for human carcinoma.
Matsuoka
H
H
Department of Surgery, Kyushu University, Beppu, Japan.
Sugimachi
K
K
Ueo
H
H
Mori
M
M
Akiyoshi
T
T
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival; D003260:Contact Inhibition; D004273:DNA, Neoplasm / Q000096:biosynthesis; D003857:Deoxyuridine / Q000378:metabolism; D006358:Hot Temperature / Q000627:therapeutic use*; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis; D012334:RNA, Neoplasm / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured / Q000378:metabolism* / Q000473:pathology
D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; D003857:Deoxyuridine