Isocratic high performance liquid chromatographic analysis of S-adenosylmethionine and S-adenosylhomocysteine in animal tissues: the effect of exposure to nitrous oxide.
A simple isocratic high performance liquid chromatographic method for the simultaneous measurement of S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) in animal tissues is described. The direct injection of perchloric acid tissue extracts and rapid resolution of both compounds in a single run reduces any sampling and analytical errors in determining the SAM/SAH ratio, a measure of methylation reactions. The method has been used to determine changes in brain SAM/SAH ratios after exposure of rats to nitrous oxide.
Bottiglieri
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Division of Anaesthesia, Clinical Research Centre, Harrow, Middlesex, UK.
D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects*; D002851:Chromatography, High Pressure Liquid; D008099:Liver / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D008745:Methylation; D009609:Nitrous Oxide / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains; D012435:S-Adenosylhomocysteine / Q000032:analysis*; D012436:S-Adenosylmethionine / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D012436:S-Adenosylmethionine; D012435:S-Adenosylhomocysteine; D009609:Nitrous Oxide