title: Expression of photosynthesis-related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts.
abstract: We have analyzed the expression of chimeric genes in populations of protoplasts isolated from the photosynthetic and nonphotosynthetic tissues within leaves of transgenic tobacco plants and separated by fluorescence-activated cell sorting. Expression of transcriptional gene fusions controlled by promoters from photosynthesis-associated genes showed a striking dependence on cell type. These patterns of expression were preserved when the gene fusions were transfected into normal (nontransgenic) tobacco leaf protoplasts.
authors.affiliation: Department of Plant Sciences, University of Arizona, Tucson 85721.
authors.forename: K R
authors.initials: KR
authors.lastname: Harkins
authors.forename: R A
authors.initials: RA
authors.lastname: Jefferson
authors.forename: T A
authors.initials: TA
authors.lastname: Kavanagh
authors.forename: M W
authors.initials: MW
authors.lastname: Bevan
authors.forename: D W
authors.initials: DW
authors.lastname: Galbraith
mesh_terms: D001483:Base Sequence; D002678:Chimera; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects; D005796:Genes; D006540:Herbicides / Q000494:pharmacology; D008969:Molecular Sequence Data; D009029:Mosaic Viruses; D010788:Photosynthesis; D010944:Plants / Q000235:genetics*; D010947:Plants, Toxic; D011523:Protoplasts / Q000378:metabolism; D011724:Pyridazines / Q000494:pharmacology; D014026:Nicotiana / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D006540:Herbicides; D011724:Pyridazines; D004247:DNA; C008677:norflurazone