title: [Detection of antibodies against Toxoplasma gondii in human sera in immunoblot procedures].
abstract: Proteins of Toxoplasma lysate were separated by SDS-polyacrylamide gel electrophoresis with subsequent transfer to a nitrocellulose sheet by electrophoretic blotting. Patient human sera reacted predominant with protein fractions of Toxoplasma antigen of 94 kD and 35 kD. Human sera were tested by Toxoplasma-IgG ELISA and Immunofluorescence assay (IgG-IFAT, IgM-IFAT).
authors.affiliation: Institut für Medizinische Mikrobiologie, Medizinischen Akademie Magdeburg.
authors.forename: G
authors.initials: G
authors.lastname: Koch
authors.forename: A
authors.initials: A
authors.lastname: Wohlfahrth
authors.forename: W A
authors.initials: WA
authors.lastname: Müller
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D014122:Toxoplasma / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan
vernacular_title: Nachweis von Antikörpern gegen Toxoplasma gondii in Humanseren im Immunoblotverfahren.