Ultrastructural demonstration of dopamine-beta-hydroxylase immunoreactive nerve terminals on vasopressin neurons in the paraventricular nucleus of the rat by double-labeling immunocytochemistry.
The noradrenergic innervation of vasopressin (VP) neurons in the paraventricular nucleus (PVN) of the rat was studied ultrastructurally by double-labeling immunocytochemistry combining the preembedding peroxidase-antiperoxidase method for dopamine-beta-hydroxylase (DBH) with the post-embedding immunogold staining method for neurophysin II, the carrier protein of VP. DBH-like immunoreactive nerve terminals were found to make synaptic contacts with neurophysin II-like immunoreactive neuronal perikarya and their processes. This provides morphological evidence for noradrenergic control of the release of VP, at the PVN of the rat.
Ochiai
H
H
Department of Anatomy, Showa University School of Medicine, Tokyo, Japan.
Nakai
Y
Y
D000818:Animals; D004299:Dopamine beta-Hydroxylase / Q000032:analysis*; D007124:Immunoenzyme Techniques; D008297:Male; D016253:Microscopy, Immunoelectron; D009411:Nerve Endings / Q000201:enzymology* / Q000648:ultrastructure; D010286:Paraventricular Hypothalamic Nucleus / Q000201:enzymology* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D013569:Synapses / Q000201:enzymology / Q000648:ultrastructure; D014667:Vasopressins / Q000032:analysis*
D014667:Vasopressins; D004299:Dopamine beta-Hydroxylase