title: Lectin binding to injured corneal endothelium mimics patterns observed during development.
abstract: Fluorochrome conjugated lectins were used to observe cell surface changes in the corneal endothelium during wound repair in the adult rat and during normal fetal development. Fluorescence microscopy of non-injured adult corneal endothelia incubated in wheat-germ agglutinin (WGA), Concanavalin A (Con A), and Ricinus communis agglutinin I (RCA), revealed that these lectins bound to cell surfaces. Conversely, binding was not observed for either Griffonia simplicifolia I (GS-I), soybean agglutinin (SBA) or Ulex europaeus agglutinin (UEA). Twenty-four hours after a circular freeze injury, endothelial cells surrounding the wound demonstrated decreased binding for WGA and Con A, whereas, RCA binding appeared reduced but centrally clustered on the apical cell surface. Furthermore, SBA now bound to endothelial cells adjacent to the wound area, but not to cells near the tissue periphery. Neither GS-I nor UEA exhibited any binding to injured tissue. By 48 h post-injury, the wound area repopulates and endothelial cells begin reestablishing the monolayer. These cells now exhibit increased binding for WGA, especially along regions of cell-to-cell contact, whereas, Con A, RCA and SBA binding patterns remain unchanged. Seventy-two hours after injury, the monolayer is well organized with WGA, Con A and RCA binding patterns becoming similar to those observed for non-injured tissue. However, at this time, SBA binding decreases dramatically. By 1 week post-injury, binding patterns for WGA, ConA and RCA closely resemble their non-injured counterparts while SBA continues to demonstrate low levels of binding. In early stages of its development, the endothelium actively proliferates and morphologically resembles adult tissue during wound repair.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Biological Sciences, Oakland University, Rochester, MI 48309-4401.
authors.forename: S R
authors.initials: SR
authors.lastname: Gordon
authors.forename: J
authors.initials: J
authors.lastname: Marchand
mesh_terms: D000818:Animals; D003315:Cornea / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D004728:Endothelium, Corneal / Q000196:embryology / Q000378:metabolism*; D005131:Eye Injuries / Q000378:metabolism*; D005333:Fetus / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D037102:Lectins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects
chemical_list: D005456:Fluorescent Dyes; D037102:Lectins