The effect of ADH and insulin on active sodium transport across frog skin in the presence of alternariol mycotoxin.
1. The effect of vasopressin and insulin on active sodium transport across frog skin in the presence of internal alternariol mycotoxin was studied, using the short-circuit technique. 2. Vasopressin stimulates sodium transport across frog skin by decreasing the resistance to sodium entry into the epithelial cells, thus partially removing the inhibition on the short-circuit current due to the action of Alternariol mycotoxin. 3. Even insulin which is known to increase the short-circuit current by a different mechanism, determines a rapid reversal effect on the inhibition due to Alternariol. 4. These data confirm the different action of the two hormones on active sodium transport across frog skin, and furthermore are indicative of an inhibition of transepithelial sodium transport by Alternariol mycotoxin probably via the sodium pump.
Micelli
S
S
Dipartimento Farmaco-Biologico, Università di Bari, Italy.
Bottalico
A
A
Gallucci
E
E
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D007783:Lactones / Q000494:pharmacology*; D011893:Rana esculenta; D012867:Skin / Q000187:drug effects / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D014667:Vasopressins / Q000494:pharmacology*
D007328:Insulin; D007783:Lactones; D014667:Vasopressins; D012964:Sodium; C005197:alternariol