Effects of trypsin on X-ray-induced cell killing, chromosome abnormalities and kinetics of DNA repair in mammalian cells.
When cells are trypsinized before irradiation a potentiation of X-ray damage may occur. This is known as the 'trypsin effect'. Potentiation of X-ray damage on cell killing was seen in V79 Chinese hamster cells but was marginal in Chinese hamster ovary (CHO K1) cells and not evident in murine Ehrlich ascites tumour (EAT) cells. Trypsinization did however increase the number of X-ray-induced chromosomal abnormalities in all 3 lines. To investigate the possibility that trypsin acts by digestion of proteins in chromatin, further experiments were performed to monitor DNA damage and repair. Induction of DNA breaks by X-rays was unaffected by trypsin but trypsinized EAT (suspension) cells repaired single-strand breaks (ssb) less rapidly than controls indicating an inhibitory effect of trypsin on ssb repair. However double-strand break (dsb) repair was unaffected by trypsin. It was also found that the EDTA solution in which the trypsin was dissolved also contributes to the inhibition of dsb repair. The results show that trypsinization can enhance X-ray-induced cell killing, chromosomal damage and DNA repair, the effect varying between cell lines.
Sprunt
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EA
Department of Biology and Preclinical Medicine, University of St. Andrews, Great Britain.
Bryant
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PE
D000818:Animals; D002453:Cell Cycle; D002460:Cell Line; D002470:Cell Survival; D002843:Chromatin / Q000187:drug effects; D002875:Chromosomes / Q000187:drug effects* / Q000528:radiation effects; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D004260:DNA Repair / Q000187:drug effects* / Q000528:radiation effects; D005374:Filtration; D007700:Kinetics; D008322:Mammals / Q000235:genetics; D014357:Trypsin / Q000633:toxicity*; D014965:X-Rays
D002843:Chromatin; D004247:DNA; D014357:Trypsin