title: Stoichiometry of phosphocreatine and inorganic phosphate changes in rat skeletal muscle.
abstract: The apparent T1's of phosphate metabolites were measured during and after series of twitch contractions in gastrocnemius muscles of pentobarbital-anesthetized rats by steady-state progressive saturation using spatially-selective composite pulses. There was no significant change in apparent T1's of inorganic phosphate (Pi), phosphocreatine (PCr), or the three phosphates of ATP. There was a 5-10% decrease in the sum of Pi and PCr integrals, and in the sum of the gamma- and beta- phosphates of ATP during stimulation, but no significant change in the ratio (Pi + PCr)/(gamma-ATP + beta-ATP). The results indicate that there is no selective decrease in NMR observable Pi during or after a series of muscle contractions.
authors.affiliation: Department of Physiology, Michigan State University, East Lansing 48824.
authors.forename: R A
authors.initials: RA
authors.lastname: Meyer
authors.forename: G R
authors.initials: GR
authors.lastname: Adams
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D004558:Electric Stimulation; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009119:Muscle Contraction / Q000502:physiology*; D009132:Muscles / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D010725:Phosphocreatine / Q000378:metabolism*; D010758:Phosphorus; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D010710:Phosphates; D010725:Phosphocreatine; D010758:Phosphorus; D000255:Adenosine Triphosphate