A single-unit recording system, contact thermal probe and electromechanical stimulator for studying cellular mechanisms related to nociception at brain stem level of awake, freely moving rats.
The purpose of this paper is to describe a simple, light-weight (3 g) device bearing a fine platinum-irridium Teflon-coated wire (50 microns) used to record single-unit activity extracellularly at brain stem level in the totally conscious freely moving rat. The up and down movements of the electrode through a guide cannula are insured by a small nut and a spring; the distance between the electrode and the end of the guide cannula is measured with a nut index. The system is directly connected to an amplifier (no FET or preamplifier) and allows for long term recordings necessary for a complete neuronal characterization and pharmacological experiments. The device is easy to make, entirely recoverable, and can be implanted from an animal to another. Further improvements are possible such as tungsten microelectrodes and telemetric or microinjection systems. In order to study some neuronal brain stem mechanisms involved in nociception, we have also designed a contact thermal probe and an electromechanical stimulator. The thermode is stuck to the shaved skin on the back of the rat, allowing heat pulses up to 51 degrees C to be applied. The mechanical stimulator is used manually and delivers reproducible innocuous stimuli to the skin. The fact that both types of stimulations are driven electrically enables the elaboration of cumulated peristimulus histograms which will reflect the neuronal activities in response to the application of noxious and non noxious stimuli.
Olivéras
J L
JL
Unité de Neurophysiologie Pharmacologique de l'INSERM (U.161), Paris, France.
Martin
G
G
Vos
B
B
Montagne
J
J
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001933:Brain Stem / Q000187:drug effects / Q000502:physiology / Q000503:physiopathology*; D004558:Electric Stimulation / Q000295:instrumentation / Q000379:methods; D008723:Methohexital / Q000494:pharmacology; D009020:Morphine / Q000494:pharmacology; D009043:Motor Activity; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D010146:Pain / Q000503:physiopathology*; D051381:Rats; D014851:Wakefulness
D009020:Morphine; D008723:Methohexital