Genomic sequencing of the 5'-flanking region of the mouse beta-globin major gene in expressing and nonexpressing mouse cells.
Genomic sequencing of two CG sites located in the 5'-flanking promoter region of the mouse beta-globin major gene shows these sites to be heavily methylated in the DNA from L929 mouse fibroblasts, a cell line that does not express the beta-globin gene. By contrast, the same CG sites in the DNA obtained from murine erythroleukemia cells, which can express the beta-globin gene, are unmethylated. The results suggest that either differentiation of progenitor cells to form the erythroid precursor murine erythroleukemia cell and/or transformation by the Friend virus group leads to demethylation of these CG sites. Final activation of the transcription process for the mouse beta-globin gene does not require demethylation in the 5'-promoter region of the DNA.
Ward
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C
Department of Cell and Developmental Biology, Roche Institute of Molecular Biology, Nutley, New Jersey 07110.
Bolden
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Weissbach
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D044503:5-Methylcytosine; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003596:Cytosine / Q000031:analogs & derivatives / Q000032:analysis; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D015870:Gene Expression; D005796:Genes; D005914:Globins / Q000235:genetics*; D004915:Leukemia, Erythroblastic, Acute; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping
D004273:DNA, Neoplasm; D044503:5-Methylcytosine; D003596:Cytosine; D005914:Globins