Isolation and characterization of blood group N antigen precursor glycoproteins with Thomsen-Friedenreich (T) activity, N antigen precursor glycoproteins and T-active glycoproteins from cyst fluids of malignant ovarian clear cell carcinoma.
1. Two perchloric acid-soluble glycoprotein fractions from cyst fluids of 2 patients with ovarian clear cell carcinoma in malignant were subjected to a systematic affinity chromatography using Vicia unijuga lectin-Cellulofine column and Arachis hypogaea lectin-Cellulofine column and separated into four glycoproteins, blood group N antigen precursor glycoprotein with Thomsen-Friedenreich (T) activity, N antigen precursor glycoprotein, T-active glycoprotein and serologically inactive glycoprotein, respectively. 2. These serologically active glycoproteins isolated in yields of 0.3-3.3% of PASFs, were proved to be mucin-type glycoproteins with Mw of 509,000-2,772,000 Da and contained 33.9-81.7% carbohydrates.
Yanagi
K
K
Department of Biochemistry, Tokyo College of Pharmacy, Japan.
Ohyama
K
K
Yamakawa
T
T
Ohkuma
S
S
D000116:Acetylgalactosamine / Q000032:analysis; D000230:Adenocarcinoma / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000276:immunology / Q000302:isolation & purification*; D015295:Antigens, Tumor-Associated, Carbohydrate; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D004187:Disaccharides / Q000276:immunology / Q000302:isolation & purification*; D005260:Female; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D037102:Lectins; D008951:MNSs Blood-Group System / Q000276:immunology*; D008358:Mannose / Q000032:analysis; D008970:Molecular Weight; D010051:Ovarian Neoplasms / Q000276:immunology*; D019887:Peanut Agglutinin; D037121:Plant Lectins; D011498:Protein Precursors / Q000276:immunology / Q000302:isolation & purification
D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D004187:Disaccharides; D006023:Glycoproteins; D037102:Lectins; D008951:MNSs Blood-Group System; D019887:Peanut Agglutinin; D037121:Plant Lectins; D011498:Protein Precursors; C042242:Vicia lectins; C016467:Thomsen-Friedenreich antigen; D000116:Acetylgalactosamine; D008358:Mannose