title: Stimulation of protein synthesis by raised extracellular pH in cardiac myocytes and perfused hearts.
abstract: Protein synthesis was stimulated in freshly-isolated rats cardiac myocytes by increasing the extracellular pH of Hepes-buffered Tyrode's solutions over the range pH 7.4-8.4. The maximal stimulation was about 45%. Protein synthesis in anterogradely-perfused rat hearts was stimulated by 11% by increasing the pH of the bicarbonate-containing perfusion medium from pH 7.4 to 7.8. This manoeuvre increased intracellular pH by 0.12 units. A concomitant increase in phosphocreatine concentration was observed. These findings are consistent with the hypothesis that intracellular pH may exert profound effects on tissue protein synthesis rates.
authors.affiliation: Department of Cardiac Medicine, University of London, England.
authors.forename: C J
authors.initials: CJ
authors.lastname: Gaitanaki
authors.forename: P H
authors.initials: PH
authors.lastname: Sugden
authors.forename: S J
authors.initials: SJ
authors.lastname: Fuller
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D001639:Bicarbonates / Q000494:pharmacology; D002021:Buffers; D006863:Hydrogen-Ion Concentration; D009200:Myocardial Contraction / Q000502:physiology; D009206:Myocardium / Q000378:metabolism*; D010477:Perfusion; D010725:Phosphocreatine / Q000378:metabolism; D014176:Protein Biosynthesis; D051381:Rats
chemical_list: D000227:Adenine Nucleotides; D001639:Bicarbonates; D002021:Buffers; D010725:Phosphocreatine