Effect of testosterone on regulation of the level of sex steroid-binding protein mRNA in monkey (Macaca fascicularis) liver.
Two specific oligonucleotide probes complementary to different regions of human sex steroid-binding protein (SBP) cDNA were used to study the levels of hepatic monkey SBP mRNA during different hormonal states. In females the SBP mRNA level was higher than in males and paralleled the serum SBP level. After castration, the SBP concentration increased in the serum but was reduced after testosterone treatment. In contrast, the hepatic SBP mRNA level decreased after castration and was restored by testosterone treatment. These results suggest a high homology of the nucleotide sequence between human and monkey SBP mRNAs. The changes in liver SBP mRNA levels may explain the sex difference in plasma SBP concentrations, but mechanisms other than the regulation of transcription may regulate the plasma concentration in monkeys.
Kottler
M L
ML
Laboratoire de Biochimie Endocrinienne, Paris, France.
Dang
C D
CD
Salmon
R
R
Counis
R
R
Degrelle
H
H
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008252:Macaca fascicularis; D008297:Male; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000378:metabolism*; D012727:Sex Characteristics; D012738:Sex Hormone-Binding Globulin / Q000235:genetics / Q000378:metabolism*; D013739:Testosterone / Q000494:pharmacology* / Q000502:physiology
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D012738:Sex Hormone-Binding Globulin; D013739:Testosterone; D004247:DNA