title: Isoelectrical focusing of connectin by agarose gel electrophoresis.
abstract: Two-dimensional gel electrophoresis using 0.5% agarose gel in the 1st dimension and 2-12% gradient polyacrylamide gel in the 2nd dimension succeeded in the isoelectrical focusing of connectin, a giant myofibrillar protein of approximately 3,000 kDa. Immunoblotting with an anti-connectin monoclonal antibody, SM1-36-2, following the two-dimensional gel electrophoresis, demonstrated that connectin was an acidic protein with an estimated pI of approximately 5.7.
authors.affiliation: Department of Neurology, Shimoshizu National Hospital, Chiba.
authors.forename: K
authors.initials: K
authors.lastname: Matsumura
authors.forename: S
authors.initials: S
authors.lastname: Hori
authors.forename: H
authors.initials: H
authors.lastname: Sugiura
authors.forename: T
authors.initials: T
authors.lastname: Shimizu
mesh_terms: D000818:Animals; D064211:Connectin; D004587:Electrophoresis, Agar Gel; D015180:Electrophoresis, Gel, Two-Dimensional; D007525:Isoelectric Focusing; D009124:Muscle Proteins / Q000032:analysis*; D009132:Muscles / Q000737:chemistry*; D011494:Protein Kinases; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D064211:Connectin; D009124:Muscle Proteins; D011494:Protein Kinases