Rapid automated analysis of glutathione reductase, peroxidase, and S-transferase activity: application to cisplatin-induced toxicity.
We describe a rapid kinetic method for the automated determination of the xenobiotic-metabolizing enzymes glutathione reductase, glutathione peroxidase and glutathione S-transferase, and its application to the study of cisplatin-induced toxicity. Liver, kidney and urine from control and cisplatin-treated rats were used as the source of enzymes. Advantages over conventional spectrophotometric methods include speed (25 assays in 4 min), small sample size, and improved precision. We show that glutathione S-transferase activity in liver is slightly reduced by cisplatin treatment, whereas all three enzymes are reduced in the kidney. Glutathione-S-transferase activity appeared in urine between the third and seventh days after cisplatin injection. Using these enzyme activities in cisplatin-treated rats, we suggest that the renal enzymes are more sensitive markers of toxicity than hepatic enzymes.
Bompart
G J
GJ
INSERM U 133, Toulouse, France.
Prévot
D S
DS
Bascands
J L
JL
D000818:Animals; D001322:Autoanalysis; D002945:Cisplatin / Q000633:toxicity*; D004796:Clinical Enzyme Tests / Q000379:methods*; D005979:Glutathione Peroxidase / Q000032:analysis*; D005980:Glutathione Reductase / Q000032:analysis*; D005982:Glutathione Transferase / Q000032:analysis* / Q000652:urine; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008297:Male; D051381:Rats; D011914:Rats, Inbred BN; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000295:instrumentation
D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D002945:Cisplatin