Detoxification of secondary products of lipid peroxidation in the cytosol of a mouse fibroblast cell line.
A cytosolic fraction prepared from a mouse fibroblast cell line reduced secondary products of lipid peroxidation, including alkenals, alkanals, alk-2-enals, and alka-2,4-dienals, using NADH or NADPH. An alternative route for detoxification of alk-2-enals and alka-2,4-dienals in the cell line cytosol was via glutathione conjugation. The major glutathione transferase in the cell line cytosol was partially characterized by glutathione-agarose chromatography and chromatofocusing. High glutathione peroxidase activity suggested that the enzyme was an alpha family glutathione transferase. The major glutathione transferase also efficiently catalyzed the conjugation of alk-2-enals and alka-2,4-dienals with glutathione.
Brophy
P M
PM
Department of Biological Sciences, University College of Wales, Aberystwyth, Dyfed, U.K.
Barrett
J
J
D000818:Animals; D002460:Cell Line; D003600:Cytosol / Q000201:enzymology; D005347:Fibroblasts / Q000201:enzymology*; D005982:Glutathione Transferase / Q000302:isolation & purification; D015227:Lipid Peroxidation / Q000502:physiology*; D051379:Mice
D005982:Glutathione Transferase