A potent thromboxane formation inhibitor in green tea leaves.
A ninhydrin positive compound (L2) now identified as 2-amino-5-(N-ethylcarboxyamido)-pentanoic acid, from unprocessed tea leaves was a potent inhibitor of thrombin-stimulated thromboxane formation in rabbit whole blood (Ali and Afzal; Prostaglandins, Leukotrienes and Medicine, 27: 9, 1987). In the present study, processed and unprocessed tea leaf extracts were given to rats to consume for a period of eight weeks. Cholesterol and thromboxane levels were measured in the serum obtained from clotting the blood at 37 degrees C. A significant reduction in thromboxane levels was observed in rats taking unprocessed tea extract. This reduction was equally distributed in adult as well as in juvenile rats. However no appreciable changes in the levels of thromboxane were noticed in the serum of rats taking processed tea extracts. This might be due to the presence of a labile component which is destroyed during the processing of green tea leaves. A decreased level of cholesterol was observed in rats consuming unprocessed tea extract. This decrease could be linked to the decrease in thromboxane levels as observed. Processed tea refers to commercially available tea of different brands while unprocessed tea refers to dried green tea leaves.
Ali
M
M
Department of Biochemistry, Faculty of Science, Kuwait University, Safat.
Afzal
M
M
Gubler
C J
CJ
Burka
J F
JF
D000596:Amino Acids / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D002784:Cholesterol / Q000097:blood; D005260:Female; D005511:Food Handling; D051381:Rats; D011919:Rats, Inbred Strains; D013662:Tea / Q000032:analysis; D013923:Thromboembolism / Q000517:prevention & control; D013929:Thromboxane B2 / Q000096:biosynthesis* / Q000097:blood
D000596:Amino Acids; D013662:Tea; C066915:2-amino-5-(N-ethylcarboxyamido)pentanoic acid; D013929:Thromboxane B2; D002784:Cholesterol