title: Loss of the Y chromosome in meningiomas. A molecular genetic approach.
abstract: Loss of the Y chromosome in meningiomas from 17 male patients was examined by cytogenetic analysis and by Southern blot hybridization with a series of Y-specific DNA probes. Cytogenetic analysis revealed loss of the Y chromosome in seven of 17 (41%) of the tumors whereas Southern blot hybridization showed loss of Y-associated sequences in only three of 17 (18%). Although the incidence of Y-chromosome loss was less by Southern blot hybridization than by cytogenetic analysis, the finding that loss of Y is present in the original uncultured tumor specimen suggests that a gene or genes on the Y chromosome may play a role in growth control of meningioma cells, and loss of this gene may be associated with tumor progression. The difference in the incidence of Y loss between the two methods indicates that both methods should be used when examining chromosome losses.
authors.affiliation: Department of Surgery, Massachusetts General Hospital, Boston 02114.
authors.forename: J A
authors.initials: JA
authors.lastname: Logan
authors.forename: B R
authors.initials: BR
authors.lastname: Seizinger
authors.forename: L
authors.initials: L
authors.lastname: Atkins
authors.forename: R L
authors.initials: RL
authors.lastname: Martuza
mesh_terms: D000328:Adult; D000368:Aged; D015139:Blotting, Southern; D002872:Chromosome Deletion; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000235:genetics; D006801:Humans; D007621:Karyotyping; D008297:Male; D008577:Meningeal Neoplasms / Q000235:genetics*; D008579:Meningioma / Q000235:genetics*; D008875:Middle Aged; D014998:Y Chromosome
chemical_list: D015342:DNA Probes; D004273:DNA, Neoplasm