Attenuation of Borrelia anserina by serial passage in liquid medium.
Borrelia anserina (Sakharoff) was successfully grown in a liquid medium (Barbour-Stoenner-Kelly) for 39 passages. By the 12th serial passage in medium, infectivity of B anserina for chicks was lost. Electron microscopy did not reveal structural differences between non-infective and infective cultured organisms. Changes in the protein profiles were found by electrophoresis as the organisms were passed in culture.
Levine
J F
JF
Department of Microbiology, Pathology and Parasitology, North Carolina State University, Raleigh 27606.
Dykstra
M J
MJ
Nicholson
W L
WL
Walker
R L
RL
Massey
G
G
Barnes
H J
HJ
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D001898:Borrelia / Q000032:analysis / Q000254:growth & development / Q000472:pathogenicity* / Q000648:ultrastructure; D001899:Borrelia Infections / Q000382:microbiology / Q000662:veterinary*; D002645:Chickens; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D008854:Microscopy, Electron; D011201:Poultry Diseases / Q000382:microbiology*; D012692:Serial Passage
D001426:Bacterial Proteins; D003470:Culture Media