title: Bovine trypsinogen activation. A thermodynamic study.
abstract: The N-alpha-L-isoleucyl-L-valine (Ile-Val) activating dipeptide, sequentially homologous to the Ile 16-Val 17 N-terminus of bovine beta-trypsin, displays an activating effect on equilibria involved in the binding of strong ligands (i.e., n-butylamine and the porcine pancreatic secretory trypsin inhibitor (Kazal-type inhibitor, type I; PSTI)) to bovine trypsinogen. This property has been investigated between pH 3.0 and 9.0 (I = 0.1 M) at 21.0 degrees C. The thermodynamics for the interaction of strong ligands with bovine beta-trypsin has also been studied under the same experimental conditions. The equilibria involved in the binding of the Ile-Val activating dipeptide and/or inhibitors to bovine beta-trypsin and its zymogen are described according to linkage relationships, wherefore interaction(s) between different functional and structural domains of the (pro)enzyme (i.e., the so-called Ile-Val pocket and the primary and/or secondary recognition subsite(s)), possibly involved in the bovine trypsinogen-to-beta-trypsin activation pathway, are considered.
authors.affiliation: Department of Biochemical Sciences, University of Rome La Sapienza, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Coletta
authors.forename: P
authors.initials: P
authors.lastname: Ascenzi
authors.forename: G
authors.initials: G
authors.lastname: Amiconi
authors.forename: M
authors.initials: M
authors.lastname: Bolognesi
authors.forename: M
authors.initials: M
authors.lastname: Guarneri
authors.forename: E
authors.initials: E
authors.lastname: Menegatti
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D004151:Dipeptides / Q000494:pharmacology*; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008024:Ligands; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011485:Protein Binding; D011487:Protein Conformation; D013816:Thermodynamics; D014362:Trypsinogen / Q000378:metabolism*
chemical_list: D004151:Dipeptides; D008024:Ligands; C041115:isoleucylvaline; D014362:Trypsinogen