An anti-HLA class I monoclonal antibody alters the progression in the cell cycle of phytohemagglutinin-activated human T lymphocytes.
The monomorphic anti-HLA Class I monoclonal antibody 01.65 inhibits the incorporation of tritiated thymidine ([3H]TdR) in Phytohemagglutinin (PHA)-activated human T lymphocytes. Our data indicate that 01.65 affects the average duration of the cell cycle by increasing the length of the early S subphase. As a consequence of the increase in the doubling time of the cell population, the absolute number of cells at harvesting time was reduced in 01.65-treated cultures compared to that of untreated cultures. The lengthening of the S-phase and the decrease in the cell number can together quantitatively account for the reduction of [3H]TdR incorporation observed in 01.65-treated cultures.
Rubini
M
M
Instituto di Genetica Medica, Università di Ferrara, Italy.
Panozzo
M
M
Selvatici
R
R
Baricordi
O R
OR
Mazzilli
M C
MC
Pozzan
T
T
Gandini
E
E
D000911:Antibodies, Monoclonal / Q000276:immunology*; D001973:Bromodeoxyuridine; D002453:Cell Cycle / Q000187:drug effects; D002478:Cells, Cultured; D004261:DNA Replication; D005434:Flow Cytometry; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D007700:Kinetics; D008213:Lymphocyte Activation; D010835:Phytohemagglutinins / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology
D000911:Antibodies, Monoclonal; D015395:Histocompatibility Antigens Class I; D010835:Phytohemagglutinins; D001973:Bromodeoxyuridine