Preparation of murine monoclonal antibodies against the yeast phase of the dimorphic fungus Sporothrix schenckii.
Three murine monoclonal antibodies (Mabs) were raised against a cytoplasmic antigen of the yeast phase of the pathogenic fungus Sporothrix schenckii using a modification of standard hybridoma technology incorporating the immunosuppressive drug cyclophosphamide. When tested for species-specificity within the pathogenic dimorphic fungi one of these Mabs (S5) showed little cross-reactivity by enzyme-linked immunosorbent assay and Western blot, though there was some recognition of Paracoccidioides brasiliensis antigen. This Mab recognized a 70-75 kDa molecule on reduced Western blots of S. schenckii antigen. The other two Mabs (S12 and S15) showed cross-reactivity with all dimorphic fungal antigens tested, though they appeared to recognize a molecule of similar molecular weight. This is the first report of any attempt to raise species-specific Mabs against this important causative agent of dermatological disease.
Hamilton
A J
AJ
Department of Clinical Sciences, Guy's Hospital, London Bridge, UK.
Harada
T
T
Bartholomew
M A
MA
Figueroa
J I
JI
Fenelon
L
L
Hay
R J
RJ
D000818:Animals; D000908:Antibodies, Fungal / Q000096:biosynthesis*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013045:Species Specificity; D013175:Sporothrix / Q000276:immunology*
D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal