Isoelectrical focusing of connectin by agarose gel electrophoresis.
Two-dimensional gel electrophoresis using 0.5% agarose gel in the 1st dimension and 2-12% gradient polyacrylamide gel in the 2nd dimension succeeded in the isoelectrical focusing of connectin, a giant myofibrillar protein of approximately 3,000 kDa. Immunoblotting with an anti-connectin monoclonal antibody, SM1-36-2, following the two-dimensional gel electrophoresis, demonstrated that connectin was an acidic protein with an estimated pI of approximately 5.7.
Matsumura
K
K
Department of Neurology, Shimoshizu National Hospital, Chiba.
Hori
S
S
Sugiura
H
H
Shimizu
T
T
D000818:Animals; D064211:Connectin; D004587:Electrophoresis, Agar Gel; D015180:Electrophoresis, Gel, Two-Dimensional; D007525:Isoelectric Focusing; D009124:Muscle Proteins / Q000032:analysis*; D009132:Muscles / Q000737:chemistry*; D011494:Protein Kinases; D051381:Rats; D011919:Rats, Inbred Strains
D064211:Connectin; D009124:Muscle Proteins; D011494:Protein Kinases