title: The effect of ischemia on glycogen phosphorylase activity in rat liver: a quantitative histochemical study.
abstract: The effects of ischemia in vitro for 0-60 min at 37 degrees C on glycogen phosphorylase activity in rat liver have been studied under different feeding conditions. Glycogen phosphorylase activity was demonstrated with a recently developed quantitative histochemical method using a semipermeable membrane and the PAS-reaction. The cytophotometrically measured glycogen phosphorylase activity in livers from 24 h-fasted rats was approximately five times the activity in livers from normally fed rats. The activity in periportal areas was about 1.5 times higher than the activity in pericentral areas in livers from starved rats, but more or less evenly distributed in livers from fed rats. Enzyme activity in pericentral areas of livers from 24 h-fasted rats started to decrease after 20 min of ischemia. After 50-60 min of ischemia, the activity was decreased to approximately 25% of the control activity. Livers from normally fed rats showed unchanged activity in periportal and pericentral areas after 10-60 min of ischemia. It has been assumed that the activation of the enzyme was disturbed by ischemia, possibly as a consequence of plasma membrane damage.
authors.affiliation: Laboratory of Cell Biology and Histology, University of Amsterdam, The Netherlands.
authors.forename: W M
authors.initials: WM
authors.lastname: Frederiks
authors.forename: F
authors.initials: F
authors.lastname: Marx
mesh_terms: D000818:Animals; D005215:Fasting / Q000378:metabolism; D006651:Histocytochemistry; D066298:In Vitro Techniques; D007511:Ischemia / Q000201:enzymology*; D008099:Liver / Q000098:blood supply / Q000201:enzymology*; D008112:Liver Glycogen / Q000378:metabolism; D008297:Male; D006005:Phosphorylases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D008112:Liver Glycogen; D006005:Phosphorylases