Mitogenic action of interleukin-1 alpha on vascular smooth muscle cells mediated by PDGF.
We have investigated the effect of interleukin-1 (IL-1) on the growth of vascular smooth muscle cells (VSMC) isolated from rat aortae. Murine recombinant IL-1 alpha increased tritiated leucine incorporation into VSMC. IL-1 also stimulated tritiated thymidine uptake by VSMC in a dose-dependent manner. On the other hand, Ca2(+)-channel blocker, verapamil, inhibited the IL-1-induced thymidine uptake by VSMC with an IC50 of 10(-8) M. Antibody specific for platelet-derived growth factor (PDGF) also totally inhibited the IL-1-induced thymidine uptake. IL-1 showed no effects on the intracellular Ca2+ level in VSMC. Above results support the premise that IL-1 promotes the growth of VSMC via induction of endogenous PDGF production and might thus participate in the abnormal proliferation of VSMC that occurs early in atherogenesis.
Ikeda
U
U
Department of Cardiology, Jichi Medical School, Tochigi, Japan.
Ikeda
M
M
Oohara
T
T
Kano
S
S
Yaginuma
T
T
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001011:Aorta / Q000166:cytology; D002118:Calcium / Q000378:metabolism; D002455:Cell Division; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D007375:Interleukin-1 / Q000494:pharmacology / Q000502:physiology*; D007930:Leucine / Q000378:metabolism; D008297:Male; D008934:Mitogens; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000276:immunology / Q000502:physiology*; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins; D013936:Thymidine / Q000378:metabolism; D014700:Verapamil / Q000494:pharmacology
D000906:Antibodies; D007375:Interleukin-1; D008934:Mitogens; D010982:Platelet-Derived Growth Factor; D011994:Recombinant Proteins; D004247:DNA; D014700:Verapamil; D007930:Leucine; D002118:Calcium; D013936:Thymidine