Expression of transferrin and vitamin D-binding protein genes in an osteogenic sarcoma cell line.
Expression of genes encoding transferrin and the vitamin D-binding protein is described in a cell line, U-2 OS, derived from a human osteogenic sarcoma. The mRNA transcripts of transferrin and vitamin D-binding protein were shown to be the lengths of those found in normal human liver. The cells synthesize and secrete the transferrin and vitamin D-binding proteins, in addition to human albumin and ceruloplasmin. The U-2 OS cells were successfully transfected with chimeric genes carrying 670 bp of the 5' regulatory sequence of the human transferrin gene fused to a reporter chloramphenicol acetyltransferase gene. These data indicate that the appropriate transcriptional factors required for expression of four plasma proteins are produced by U-2 OS nuclei and that the U-2 OS cell line will be useful for studies analyzing regulation of these genes.
Adrian
G S
GS
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284.
Yang
F M
FM
Graves
D T
DT
Buchanan
J M
JM
Bowman
B H
BH
D002570:Ceruloplasmin / Q000096:biosynthesis; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003001:Cloning, Molecular; D006801:Humans; D012516:Osteosarcoma / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D012709:Serum Albumin / Q000096:biosynthesis; D014158:Transcription, Genetic; D014162:Transfection; D014168:Transferrin / Q000096:biosynthesis / Q000235:genetics*; D014407:Tumor Cells, Cultured; D014809:Vitamin D-Binding Protein / Q000096:biosynthesis / Q000235:genetics*
D012333:RNA, Messenger; D012709:Serum Albumin; D014168:Transferrin; D014809:Vitamin D-Binding Protein; D002570:Ceruloplasmin; D015500:Chloramphenicol O-Acetyltransferase