Use of radio-gas chromatography and tritium label to optimize derivatization procedures.
A sensitive determination of gas chromatographic peak yields by a new radio-gas chromatograph equipped with a synchronized accumulating radioisotope detector is described. Peak yields could be easily determined by the radio-gas chromatograph using [3H]hexadecane or [3H]androstenedione as a standard substance. The usefulness of the determination of peak yields was demonstrated by optimizing the derivatization conditions of 6-keto-prostaglandin F1 alpha.
Baba
S
S
Tokyo College of Pharmacy, Japan.
Akira
K
K
Seyama
Y
Y
D015121:6-Ketoprostaglandin F1 alpha / Q000032:analysis* / Q000737:chemistry; D000473:Alkanes; D000735:Androstenedione; D002849:Chromatography, Gas / Q000379:methods* / Q000592:standards; D014316:Tritium
D000473:Alkanes; D014316:Tritium; D000735:Androstenedione; D015121:6-Ketoprostaglandin F1 alpha; C007932:n-hexadecane