title: Relation of phosphatidylinositol metabolism to glycolytic pathway in skeletal muscle membranes.
abstract: Skeletal muscle triads are possessing the whole set of enzymes of the phosphatidylinositol (PI)-linked signal generating pathway, PI-kinase, PI(4)P-kinase, and PI(4,5)P2-phospholipase C (PLC). The activities of these enzymes are comparable to those found in other cell types for which a functional role of the PI-pathway in intracellular signal transduction has been established. For skeletal muscle an unequivocal function and an initiating signal for Ins(1,4,5)P3-liberation is still unknown. However, the observed Ca-dependency of PLC activity suggests that here Ins(1,4,5)P3 production is a consequence rather than a cause of increasing cytosolic Ca2+. Recently, the glycolytic enzyme aldolase, whose activity can be modulated by inositol polyphosphates, has been localized in the triadic structure. The enzyme which has a high affinity to Ins(1,4)P2, Ins(1,4,5)P3 and Ins(1,3,4,5)P4, seems to be compartmentalized to the junctional foot structure from which it is released upon binding of these molecules. This phenomenon could reflect a capability for regulation of the glycolytic flux even for aldolase, especially if a non steady-state situation in the junctional gap is considered. Meanwhile we have accumulated evidence for the operation of a partial glycolytic sequence in the junctional region established by the enzymes aldolase, glyceraldehyde-3-P (GAP) dehydrogenase and phosphoglycerate kinase. This system is able to produce ATP upon oxidation of GAP and could be, because of the inositol polyphosphate-sensing abilities of aldolase, a target for the membrane associated PI-pathway. The ATP production is however transient which indicates the coupling to an ATP hydrolyzing reaction.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Abteilung für Biochemie Supramolekularer Systeme, Ruhr-Universität Bochum, FRG.
authors.forename: L M
authors.initials: LM
authors.lastname: Heilmeyer
authors.forename: J W
authors.initials: JW
authors.lastname: Han
authors.forename: R
authors.initials: R
authors.lastname: Thieleczek
authors.forename: M
authors.initials: M
authors.lastname: Varsanyi
authors.forename: G W
authors.initials: GW
authors.lastname: Mayr
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D006019:Glycolysis; D009132:Muscles / Q000378:metabolism* / Q000648:ultrastructure; D010716:Phosphatidylinositols / Q000378:metabolism*
chemical_list: D010716:Phosphatidylinositols