A rapid two-step purification of rat liver fetal thymidine kinase.
The fetal isoenzyme of thymidine kinase was purified to apparent homogeneity from cytosols of rat fetuses liver. A two-step purification including anion exchange chromatography and affinity chromatography was developed. The purified enzyme appears as oligomeric with a relative molecular weight of 71 kDa. In denaturing media its molecular weight was 24 kDa, and its pHi 8.3.
Baron
G
G
Centre Régional de Recherche en Endocrinologie, Faculté de Médecine-Pharmacie, Rennes, France.
Madec-Baron
A
A
Sampérez
S
S
Jouan
P
P
D000818:Animals; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000302:isolation & purification* / Q000378:metabolism; D008099:Liver / Q000196:embryology / Q000201:enzymology*; D008970:Molecular Weight; D051381:Rats; D013937:Thymidine Kinase / Q000302:isolation & purification* / Q000378:metabolism
D007527:Isoenzymes; D013937:Thymidine Kinase