The histochemical reaction of horseradish peroxidase in rodent 'whole-brain' preparations.
Subsequent to eye injections of horseradish peroxidase (HRP) 'whole-brain' preparations with only the cortex removed were reacted for HRP using tetramethylbenzidine (TMB) as the chromogen and ammonium heptamolybdate (AHM) as a stabilizer. Retinal projections could be photographed and visualized globally coursing across the surface of the thalamus and midbrain. The brains were then sectioned, and when necessary re-reacted, enabling a 3-D reconstruction of retinofugal pathways to be made.
Cooper
A M
AM
Department of Experimental Psychology, University of Oxford, UK.
D000818:Animals; D001560:Benzidines / Q000494:pharmacology; D001921:Brain / Q000033:anatomy & histology*; D006651:Histocytochemistry; D006735:Horseradish Peroxidase; D008982:Molybdenum / Q000494:pharmacology; D051381:Rats
D001560:Benzidines; C021758:3,3',5,5'-tetramethylbenzidine; D008982:Molybdenum; D006735:Horseradish Peroxidase; C022175:ammonium molybdate