title: [Identification and isolation of the protein insect toxin (alpha-latroinsectotoxin from venom of the spider Latrodectus mactans tredecimguttatus].
abstract: The crude venom of spider Latrodectus mactans tredecimguttatus was fractionated by the combination of anion exchange and hydrophobic chromatography. The biological activity of fraction was tested by means of: 1) estimation of toxicity for housefly larva; 2) intracellular recording of miniature excitatory potentials (MEPSPs) in blowfly larvae muscle fibres. As a result of sequential procedures of chromatography separation a homogeneous protein of 120 kilodalton molecular weight was obtained. This protein referred to alpha-latroinsectotoxin produced: 1) a great increase of the frequency of MEPSPs in the dose of 4.2.10(-10) M and its paralytic dose for fly larva was approximately 20 ng/species; 2) no influence of the MEPSPs after application in the dose of 1.2.10(-7) M to the neuromuscular junction of the frog.
authors.forename: G I
authors.initials: GI
authors.lastname: Kovalevskaia
authors.forename: V N
authors.initials: VN
authors.lastname: Pashkov
authors.forename: O V
authors.initials: OV
authors.lastname: Bulgakov
authors.forename: I M
authors.initials: IM
authors.lastname: Fedorova
authors.forename: L G
authors.initials: LG
authors.lastname: Magazanik
authors.forename: E V
authors.initials: EV
authors.lastname: Grishin
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D001001:Anura; D001740:Black Widow Spider; D002852:Chromatography, Ion Exchange; D004175:Diptera; D004591:Electrophoresis, Polyacrylamide Gel; D005071:Evoked Potentials / Q000187:drug effects; D066298:In Vitro Techniques; D013111:Spider Venoms / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity
chemical_list: D000596:Amino Acids; D013111:Spider Venoms; C020763:alpha-latrotoxin
vernacular_title: Identifikatsiia i vydelenie belkovogo insektotoksina (alpha- latroinsektotoksina) iz iada pauka Latrodectus mactans tredecimguttatus.