Comparative study of three phospholipase A2s from the venom of Vipera aspis.
1. Three phospholipase A2s, PLA2-I, PLA2-II and PLA2-III, were isolated from Vipera aspis venom by gel filtration and ion exchange chromatography. 2. Purified PLA2-I, -II and -III have mol. wts of 30,200, 16,000 and 13,500, and isoelectric points of 9.45, 7.65 and less than 4.1, respectively. 3. PLA2-I consists of an acidic subunit (mol. wt 13,700, pI: less than 3.5) and a basic subunit (mol. wt 16,500, pI: 10.6), which can be separated under highly acidic conditions. 4. PLA2-I possessed lethal activity and LD50 for this preparation was estimated to be 0.288 (0.209-0.397) micrograms/g, while lethality was not observed when PLA2-II, -III or each subunit of PLA2-I were administered. 5. Capillary permeability-increasing activity was found in the samples which possessed basic isoelectric points. Additionally, PLA2-I and its basic subunit drastically prolonged activated partial thromboplastin time of platelet rich plasma. 6. Intramuscular injections of PLA2-I, -II and -III increased serum creatine phosphokinase activity in mice, indicating that damage in muscle was caused by these enzymes. 7. NH2-terminal sequences of the three PLA2s were compared with other phospholipase A2s from snake venoms. Furthermore, antigenicities were tested using antiserum prepared against each sample.
Komori
Y
Y
Department of Microbiology, Faculty of Pharmacy, Meijo University, Nagoya, Japan.
Nikai
T
T
Sugihara
H
H
D000595:Amino Acid Sequence; D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects; D007120:Immunochemistry; D007526:Isoelectric Point; D007928:Lethal Dose 50; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009132:Muscles / Q000187:drug effects / Q000201:enzymology; D010741:Phospholipases A / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D054467:Phospholipases A2; D012689:Sequence Homology, Nucleic Acid; D014757:Viper Venoms / Q000737:chemistry*
D014757:Viper Venoms; D010741:Phospholipases A; D054467:Phospholipases A2