title: Urinary N-acetyl-beta-D-glucosaminidase isoenzyme activity as measured by fast protein liquid chromatography in patients with nephrotic syndrome.
abstract: Exactly why N-acetyl-beta-D-glucosaminidase (NAG) excretion is increased in patients with nephrotic syndrome with glomerular lesions is poorly understood. Glomeruli contain less NAG than do proximal tubules. In this study, we have tried to measure the NAG isoenzymes automatically by use of the recently developed fast protein liquid chromatography (FPLC) system, followed by column chromatography on DEAE cellulose (Mono Q). Three isoenzyme peaks--B, I + II, and A--were observed for urine from both healthy subjects and nephrotic patients. The B isoenzyme usually constituted about 10% of the total NAG in healthy controls, 30% in nephrotic patients. In contrast, the proportion of the A isoenzyme was inversely related to that of the B isoenzyme when healthy controls and nephrotic patients were compared. Our system for measuring NAG isoenzymes is reproducible and fast, and it should be useful in further studies.
authors.affiliation: Second Department of Internal Medicine, Jikei University School of Medicine, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Shibasaki
authors.forename: H
authors.initials: H
authors.lastname: Gomi
authors.forename: F
authors.initials: F
authors.lastname: Ishimoto
authors.forename: T
authors.initials: T
authors.lastname: Miyahara
mesh_terms: D000118:Acetylglucosaminidase / Q000652:urine*; D001322:Autoanalysis; D002853:Chromatography, Liquid / Q000379:methods; D005260:Female; D006596:Hexosaminidases / Q000652:urine*; D006801:Humans; D007527:Isoenzymes / Q000652:urine*; D008297:Male; D009404:Nephrotic Syndrome / Q000201:enzymology / Q000652:urine*
chemical_list: D007527:Isoenzymes; D006596:Hexosaminidases; D000118:Acetylglucosaminidase