Molecular analysis of the REV2 gene of Saccharomyces cerevisiae--a review.
The REV2 gene controls DNA repair, induced mutagenesis and, probably, some fidelity mechanism of replication. Of particular interest is the notion that it is inducible by DNA-damaging agents. We wanted to find molecular evidence for these results derived from numerous biological experiments. We cloned the REV2 gene from a yeast genomic DNA library based on the YCp50 centromere vector, sequenced it and studied its regulation on the transcriptional level. The coding region of the REV2 gene consists of a 1425 pb reading frame with a coding capacity for a polypeptide of 52 kD; no significant homology to any gene filed in available data bases was found. Examination of a hydrophobicity plot of the putative Rev2 protein predicts the existence of transmembrane helices. Quantitative Northern analysis confirmed the working hypothesis that DNA-damaging agents increase the level of REV2 gene expression in stationary cells. Thus, the REV2 gene seems to code for a membrane protein which is inducible by DNA-damaging agents and which controls processes of repair and mutagenesis in yeast.
Ahne
F
F
GSF-Institut für Strahlenbiologie, Neuherberg, Federal Republic of Germany.
Wendel
S
S
Eckardt-Schupp
F
F
D001483:Base Sequence; D003001:Cloning, Molecular; D004260:DNA Repair / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal / Q000502:physiology; D005800:Genes, Fungal / Q000502:physiology*; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics