[Increasing the toxic effect of pulmonary carcinogens by destruction of epithelial-mesenchymal interaction in explants of embryonic respiratory tract of mice].
The explants from 17-day old embryos of A and C57BL mice were used for long-term organ cultures. The following series of explants were studied: 1) the intact control explants; 2) the explants treated by NMU; 3) the explants treated by BP; 4) the explants isolated from mesenchyma (M); 5) the explants isolated from M and treated by NMU; 6) the explants isolated from M and treated by BP. The survival of explants treated by NMU or BP did not differ from the intact control explants (p less than 0.1). The removal of M decreased the survival only in the explants from the distal RT of A mice (p 0.01). The survival of explants isolated from M and treated by NMU or BP was significantly lower than it was in intact explants (p less than 0.01-0.001). Thus, the destruction of epithelial-mesenchymal interaction induced by removal of M can modulate the toxic effect of pulmonotropic carcinogens.
Kolesnichenko
T S
TS
Medvinskiĭ
A L
AL
D000818:Animals; D001580:Benzopyrenes / Q000633:toxicity*; D004622:Embryo, Mammalian; D004848:Epithelium; D005865:Gestational Age; D008168:Lung / Q000033:anatomy & histology / Q000187:drug effects*; D008770:Methylnitrosourea / Q000633:toxicity*; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D009924:Organ Culture Techniques
D001580:Benzopyrenes; D008770:Methylnitrosourea
Usilenie toksicheskogo éffekta pul'monotropnykh kantserogenov, vyzvannoe narusheniem épitelial'no-mezenkhimnogo sootnosheniia v éksplantatakh émbrional'nogo respiratornogo trakta mysheĭ.