title: Appearance of bisected N-acetylglucosamine residue of biantennary sugar chains and decrease of high molecular weight oligosaccharides of human lymphocytic cell membranes during differentiation.
abstract: Asparagine-linked sugar chains obtained from the plasma membranes of human acute lymphocytic leukemic cells, human B cells, Epstein-Barr virus (EBV)-infected B cell lines, and T cells were quantitatively liberated from the polypeptide portions by hydrazinolysis followed by N-acetylation and NaB3H4 reduction. The radioactive oligosaccharides were fractionated by high-voltage paper electrophoresis. Their structures were studied by column chromatography and sequential exoglycosidase digestion. The neutral oligosaccharides were of a high mannose type. The acidic oligosaccharides were bi-, tri-, and tetraantennary complex-type sugar chains with Man alpha----(Man alpha----) Man beta----GlcNAc beta----(+/- Fuc alpha----)GlcNAc as their cores. A comparative study of the oligosaccharides of these cells revealed that the biantennary complex-type sugar chain with bisecting N-acetylglucosamine residues was found only in B cells, B lymphoblasts, and B cell lines. High molecular weight oligosaccharides decreased during the differentiation stage of lymphocytes.
authors.affiliation: Department of Pediatrics, Gifa University School of Medicine, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Motoyoshi
authors.forename: N
authors.initials: N
authors.lastname: Kondo
authors.forename: T
authors.initials: T
authors.lastname: Orii
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism*; D001216:Asparagine / Q000378:metabolism; D001402:B-Lymphocytes / Q000166:cytology; D002454:Cell Differentiation; D002462:Cell Membrane / Q000502:physiology; D005944:Glucosamine / Q000031:analogs & derivatives*; D006801:Humans; D007945:Leukemia, Lymphoid / Q000473:pathology*; D008565:Membrane Proteins / Q000378:metabolism; D008970:Molecular Weight; D009844:Oligosaccharides / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D009844:Oligosaccharides; D001216:Asparagine; D005944:Glucosamine; D000117:Acetylglucosamine