Flow cytometric DNA content analysis in primary lung cancer: comparison of results from fresh and paraffin-embedded specimens.
Tumor DNA content was determined by flow cytometry in 24 surgically resected specimens from patients with primary lung cancer to compare results from fresh and paraffin-embedded specimens. We found a good correlation in DNA indices between fresh and paraffin-embedded specimens (r = .978), although one tumor with DNA near-diploidy was mistaken as DNA diploid in paraffin-embedded specimens. In the measurements of paraffin-embedded specimens, eight of 24 tumors were DNA multiploid and showed DNA content heterogeneity. These results indicate that DNA content analysis of paraffin-embedded specimens is helpful because it makes it easy to analyze multiple specimens from different parts of the tumor.
Isobe
H
H
First Department of Medicine, School of Medicine, Hokkaido University, Sapporo, Japan.
Miyamoto
H
H
Inoue
K
K
Shimizu
M
M
Endo
T
T
Mizuno
S
S
Kawakami
Y
Y
D000230:Adenocarcinoma / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D010232:Paraffin; D011003:Ploidies
D004273:DNA, Neoplasm; D010232:Paraffin