Morphological and biochemical examination of Cosmos 1887 rat heart tissue: Part I--Ultrastructure.
Morphological changes were observed in the left ventricle of rat heart tissue from animals flown on the Cosmos 1887 biosatellite for 12.5 days. These tissues were compared to the synchronous and vivarium control hearts. While many normal myofibrils were observed, others exhibited ultrastructural alterations, i.e., damaged and irregular-shaped mitochondria and generalized myofibrillar edema. Analysis of variance (ANOVA) of the volume density data revealed a statistically significant increase in glycogen and a significant decrease in mitochondria compared to the synchronous and vivarium controls. Point counting indicated an increase in lipid and myeloid bodies and a decrease in microtubules, but these changes were not statistically significant. In addition, the flight animals exhibited some patchy loss of protofibrils (actin and myosin filaments) and some abnormal supercontracted myofibrils that were not seen in the controls. This study was undertaken to gain insight into the mechanistic aspects of cardiac changes in both animals and human beings as a consequence of space travel (1). Cardiac hypotrophy and fluid shifts have been observed after actual or simulated weightlessness and raise concerns about the functioning of the heart and circulatory system during and after travel in space (2-4).
Philpott
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DE
NASA-Ames Research Center, Moffett Field, California 94035.
Popova
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IA
Kato
K
K
Stevenson
J
J
Miquel
J
J
Sapp
W
W
D000199:Actins / Q000378:metabolism; D000818:Animals; D006003:Glycogen / Q000378:metabolism; D050356:Lipid Metabolism; D008297:Male; D008854:Microscopy, Electron; D008929:Mitochondria, Heart / Q000648:ultrastructure; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure*; D009210:Myofibrils / Q000648:ultrastructure; D009218:Myosins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013026:Space Flight; D014893:Weightlessness
D000199:Actins; D006003:Glycogen; D009218:Myosins