Distinct tumor cell membrane constituents activate human monocytes for tumor necrosis factor synthesis.
Several lines of evidence point to an activation mechanism of monocytes/macrophages by tumor cells. In this study we present data for distinct surface structures on K562 and Jurkat cells to directly induce TNF-mRNA expression and TNF production by human peripheral blood monocytes. Northern analysis showed that incubation of monocytes with either K562 or Jurkat cells led to a significant increase in TNF-mRNA expression. In addition, enhanced TNF production was detected in supernatants of monocyte cultures activated by Jurkat cells. Not only viable tumor cells but also metabolically inactivated tumor cells, cytoblasts, and membrane preparations from Jurkat and K562 cells induced TNF-mRNA expression. We identified two different membrane protein fractions with relative molecular mass of 32 to 38 kDa for Jurkat cells and 46 to 54 kDa for K562 cells that were responsible for monocyte activation.
Jänicke
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Institute of Immunology and Genetics, German Cancer Research Center, Heidelberg.
Männel
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D015152:Blotting, Northern; D005786:Gene Expression Regulation; D006801:Humans; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation; D008565:Membrane Proteins / Q000502:physiology*; D009000:Monocytes / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000502:physiology; D009369:Neoplasms / Q000503:physiopathology*; D012333:RNA, Messenger / Q000235:genetics; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D008070:Lipopolysaccharides; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha