Liquid chromatographic determination of clobetasone-17-butyrate in ointments.
A liquid chromatographic (LC) method has been developed for determination of clobetasone-17-butyrate in ointment using clobetasone propionate as an internal standard. Separation was carried out on a C18 reverse-phase column using water-methanol as a mobile phase. Methylparaben and propylparaben (both sodium salt) used as preservatives did not interfere with separation. Compounds are detected photometrically at 235 nm. Mean assay results for 0.05% commercial ointments were 100.36% (n = 5). Mean recovery of clobetasone-17-butyrate added to commercial ointment was 99.89%.
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Cadila Laboratories Ltd, Analytical Research Laboratory, Gujarat, India.
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D000287:Administration, Topical; D000893:Anti-Inflammatory Agents / Q000032:analysis*; D002853:Chromatography, Liquid; D002990:Clobetasol / Q000031:analogs & derivatives* / Q000032:analysis; D005938:Glucocorticoids; D009824:Ointments / Q000032:analysis; D010226:Parabens / Q000032:analysis*; D011310:Preservatives, Pharmaceutical / Q000032:analysis*
D000893:Anti-Inflammatory Agents; D005938:Glucocorticoids; D009824:Ointments; D010226:Parabens; D011310:Preservatives, Pharmaceutical; C010893:clobetasone butyrate; C015358:methylparaben; D002990:Clobetasol; C006068:propylparaben