title: The stability of glutathione peroxidase activity in plasma from cattle, pigs and sheep on storage in the presence and absence of glutathione.
abstract: Ovine, bovine and porcine plasma glutathione peroxidase (GSH-Px) activity decreased on storage at both 4 degrees C and 20 degrees C. The ovine and bovine enzymes were significantly less stable than the porcine enzyme. The addition of GSH to a final concentration of 2 mmol/L to plasma samples at the commencement of storage retarded the loss of both ovine and bovine plasma GSH-Px activity. The ovine enzyme was unique in that after inactivation by storage at 4 degrees C, incubation with GSH restored the enzyme activity. It is recommended that plasma GSH-Px should be assayed fresh, or otherwise stored at -20 degrees C.
authors.affiliation: Veterinary Research Laboratories, Stormont, Belfast, UK.
authors.forename: W B
authors.initials: WB
authors.lastname: Davidson
authors.forename: D G
authors.initials: DG
authors.lastname: Kennedy
authors.forename: P J
authors.initials: PJ
authors.lastname: Hughes
authors.forename: W J
authors.initials: WJ
authors.lastname: Blanchflower
mesh_terms: D000818:Animals; D002417:Cattle / Q000097:blood*; D003080:Cold Temperature; D004789:Enzyme Activation; D004795:Enzyme Stability; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000097:blood* / Q000378:metabolism; D006207:Half-Life; D007700:Kinetics; D011309:Preservation, Biological; D012756:Sheep / Q000097:blood*; D013552:Swine / Q000097:blood*
chemical_list: D005979:Glutathione Peroxidase; D005978:Glutathione