Protein kinase-C involvement in thymocyte apoptosis induced by hydrocortisone.
The involvement of protein kinase-C in thymocytes death induced by hydrocortisone was studied. Thymus cells were incubated 6 hr or in the presence of hydrocortisone, labeled with Acridine orange, and the DNA content of each nuclei was estimated by cytofluorimetry. The results indicate that hydrocortisone-induced DNA fragmentation can be prevented by adding the protein kinase-C inhibitor H-7 to the cell suspension. Incubation of the H-A 1004, an inhibitor of c-AMP-dependent protein kinase, with low effect on on protein kinase-C, did not interfere with the cortisone-mediated DNA fragmentation. Therefore, it can be concluded that protein kinase-C plays an important role in the process of lympholysis mediated by corticoids.
Ojeda
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Department of Physics, Universidad Austral de Chile, Valdivia.
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Maldonado
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Folch
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D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000378:metabolism; D006854:Hydrocortisone / Q000494:pharmacology*; D007546:Isoquinolines / Q000494:pharmacology; D051379:Mice; D010879:Piperazines / Q000494:pharmacology; D011493:Protein Kinase C / Q000502:physiology*; D013449:Sulfonamides; D013601:T-Lymphocytes / Q000187:drug effects*
D007546:Isoquinolines; D010879:Piperazines; D013449:Sulfonamides; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D004247:DNA; C043204:N-(2-guanidinoethyl)-5-isoquinolinesulfonamide; D011493:Protein Kinase C; D006854:Hydrocortisone