title: Additional evidence of autoxidation as a possible mechanism of neutrophil locomotory dysfunction in blunt trauma.
abstract: Previous studies in victims of blunt injury suggest that the observed neutrophil (PMN) locomotory dysfunction is, in part, due to autoxidation. To further clarify the occurrence and significance of autoxidation, we studied changes in levels of glutathione in PMN and of ascorbic acid and alpha-tocopherol in serum and blood cells of postsurgical and blunt trauma patients. Levels of total, reduced, and oxidized glutathione in PMN from trauma patients were similar to normal controls. Serum and cellular ascorbic acid and alpha-tocopherol levels dropped significantly after injury and remained below normal control levels during the 7 to 8-day study period. Low serum alpha-tocopherol was partially explainable on the basis of changes in serum lipids. When serum samples of trauma patients were thawed unprotected without pyrogallol, there was significant loss of recoverable alpha-tocopherol, whereas no significant losses occurred with unprotected thawed normal sera. Less total reducing capacity was observed in PMN of trauma patients compared with normal controls. These findings indicate that synthesis and regeneration capacity of glutathione are intact but that the levels of the consumable antioxidants, ascorbic acid, and alpha-tocopherol are compromised after injury. These results add further support to the hypothesis that autoxidation occurs in trauma.
authors.affiliation: Medical Research Laboratory, Hartford Hospital, CT 06115.
authors.forename: E G
authors.initials: EG
authors.lastname: Maderazo
authors.forename: C L
authors.initials: CL
authors.lastname: Woronick
authors.forename: N
authors.initials: N
authors.lastname: Hickingbotham
authors.forename: E
authors.initials: E
authors.lastname: Mercier
authors.forename: L
authors.initials: L
authors.lastname: Jacobs
authors.forename: H
authors.initials: H
authors.lastname: Bhagavan
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001205:Ascorbic Acid / Q000097:blood; D002634:Chemotaxis, Leukocyte; D002784:Cholesterol / Q000097:blood; D005260:Female; D005978:Glutathione / Q000097:blood; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000032:analysis / Q000502:physiology*; D010084:Oxidation-Reduction; D011184:Postoperative Period; D014810:Vitamin E / Q000097:blood; D014949:Wounds, Nonpenetrating / Q000378:metabolism*
chemical_list: D014810:Vitamin E; D002784:Cholesterol; D005978:Glutathione; D001205:Ascorbic Acid