Effects of treatment with microencapsulated monosialoganglioside GM1 on cortical and striatal acetylcholine release in rats with cortical devascularizing lesions.
The present study shows a novel administration form of the monoganglioside GM1, which following microencapsulation in human serum albumin was topically applied on cortical regions damaged by devascularization in rats. The effects of microencapsulated GM1 on extracellular levels of acetylcholine, choline and dopamine in the cortex and in the striatum were analyzed using in vivo microdialysis. Cholinergic neurons in the nucleus basalis magnocellularis were studied immunohistochemically using monoclonal antibodies raised against choline acetyltransferase (ChAT). It was found that cortical devascularizing lesions produced a decrease in extracellular levels of cortical acetylcholine and choline, and retrograde morphological changes in cholinergic neurons in the nucleus basalis magnocellularis. GM1 promoted (1) recovery of the retrograde morphological changes produced by the decortication in the nucleus basalis magnocellularis and (2) a parallel increase in cortical acetylcholine release. No changes were observed in the striatum, nor on cortical or striatal dopamine levels simultaneously measured in the same perfusates.
Maysinger
D
D
Department of Pharmacology and Therapeutics, McGill University, Montreal Canada.
Herrera-Marschitz
M
M
Ungerstedt
U
U
Cuello
A C
AC
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D002214:Capsules; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism; D002541:Cerebral Decortication; D002561:Cerebrovascular Disorders / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D002794:Choline / Q000378:metabolism; D002795:Choline O-Acetyltransferase / Q000032:analysis; D003342:Corpus Striatum / Q000187:drug effects* / Q000378:metabolism; D005677:G(M1) Ganglioside / Q000008:administration & dosage* / Q000627:therapeutic use; D007150:Immunohistochemistry; D008297:Male; D008855:Microscopy, Electron, Scanning; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000008:administration & dosage
D002214:Capsules; D012709:Serum Albumin; D005677:G(M1) Ganglioside; D002795:Choline O-Acetyltransferase; D002794:Choline; D000109:Acetylcholine