title: Presence of an additional penicillin-binding protein in methicillin-resistant Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus hominis, and Staphylococcus simulans with a low affinity for methicillin, cephalothin, and cefamandole.
abstract: The presence of an additional penicillin-binding protein (PBP) was demonstrated in methicillin-resistant strains of Staphylococcus epidermidis, S. haemolyticus, S. hominis, and S. simulans. In these four species, the apparent molecular mass of this protein was analogous to that of PBP 2' of methicillin-resistant S. aureus SR 1550-9. It exhibited a low affinity for methicillin, cephalothin, and cefamandole; and its synthesis was methicillin inducible. Peptide mapping of this PBP from the four species yielded identical results that were analogous to those obtained with S. aureus SR 1550-9. These results suggest that this protein is similar to, if not the same as, PBP 2' of S. aureus and that it is involved in methicillin resistance in the four species studied.
authors.affiliation: Laboratoire de Microbiologie Médicale, Université Pierre et Marie Curie, Paris, France.
authors.forename: J
authors.initials: J
authors.lastname: Pierre
authors.forename: R
authors.initials: R
authors.lastname: Williamson
authors.forename: M
authors.initials: M
authors.lastname: Bornet
authors.forename: L
authors.initials: L
authors.lastname: Gutmann
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D002435:Cefamandole / Q000378:metabolism*; D002512:Cephalothin / Q000378:metabolism*; D006602:Hexosyltransferases; D008712:Methicillin / Q000378:metabolism*; D016106:Methicillin Resistance / Q000235:genetics / Q000502:physiology*; D002267:Muramoylpentapeptide Carboxypeptidase / Q000378:metabolism*; D046915:Penicillin-Binding Proteins; D010449:Peptide Mapping; D010458:Peptidyl Transferases; D010641:Phenotype; D013210:Staphylococcus / Q000201:enzymology / Q000378:metabolism*; D013212:Staphylococcus epidermidis / Q000201:enzymology / Q000378:metabolism*; D001618:beta-Lactamases / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D046915:Penicillin-Binding Proteins; D002435:Cefamandole; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase; D001618:beta-Lactamases; D008712:Methicillin; D002512:Cephalothin