Interaction of late murine erythroid progenitors and stroma involves a recognition mechanism with fucosyl specificity.
We have previously reported that the specific recognition and binding of murine hemopoietic progenitors spleen colony-forming units (CFU-S) and granulocyte-macrophage CFU (CFU-GM) to hemopoietic stroma is dependent upon a membrane recognition system with galactose and mannose specificities. By using synthetic neoglycoproteins with galactose, mannose, or fucose covalently bound to bovine serum albumin (BSA) in standard long-term bone marrow cultures (LTBMC), galactosyl-BSA (gal-BSA) and mannosyl-BSA (man-BSA) but not fucosyl-BSA (fuc-BSA) inhibited the binding of CFU-S and CFU-GM to the stromal layer. In the present work it was shown that binding of erythroid burst-forming units (BFU-E) to stroma in standard LTBMC is also inhibited by gal-BSA and man-BSA. We then studied a different system of LTBMC that favored erythropoiesis and allowed the production of erythroid CFU (CFU-E) for 4 weeks. In the presence of the fuc-BSA as well as gal-BSA and man-BSA, total cell production and CFU-E production were halted in the supernate as well as the adherent layer. These results indicate the presence of a fucosyl recognition system on the surface of the late erythroid precursors, CFU-E.
Konno
M
M
Veterans Administration Medical Center, Jackson, MS 39216.
Hardy
C
C
Tavassoli
M
M
D000818:Animals; D001853:Bone Marrow / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D015672:Erythroid Precursor Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004920:Erythropoiesis / Q000187:drug effects; D005643:Fucose / Q000378:metabolism* / Q000494:pharmacology; D005690:Galactose / Q000494:pharmacology; D008297:Male; D008358:Mannose / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D012709:Serum Albumin; D012710:Serum Albumin, Bovine / Q000494:pharmacology
D012709:Serum Albumin; C039136:bovine serum albumin-galactose (39); C053610:fucose-bovine serum albumin conjugate; C023840:mannose-bovine serum albumin conjugate; D012710:Serum Albumin, Bovine; D005643:Fucose; D008358:Mannose; D005690:Galactose