Characterisation of Bacteroides from sheep periodontal disease by SDS-PAGE of outer membrane proteins.
Isolates of Bacteroides species obtained from a longitudinal study of developing periodontal disease in sheep were analysed by SDS-PAGE. Protein profiles of Sarkosyl-insoluble outer membrane extracts were compared within groups of isolates which had already been defined by conventional biochemical techniques. Heterogeneity was exhibited within most groups. Isolates of B. gingivalis and B. asaccharolyticus shown to be similar to human isolates by conventional biochemical tests, gave different protein profiles from the respective type cultures. The sheep B. gingivalis-like isolates were however homogeneous, while the B. asaccharolyticus-like organisms could be divided into 3 subgroups. SDS-PAGE appears to be a useful tool for the examination of bacterial flora and recognition of subgroups of subspecies.
McCourtie
J
J
Bacteriology Department, University of Edinburgh Medical School, U.K.
Poxton
I R
IR
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D001439:Bacteroides / Q000032:analysis / Q000145:classification*; D001442:Bacteroides Infections / Q000382:microbiology / Q000662:veterinary*; D004591:Electrophoresis, Polyacrylamide Gel; D010510:Periodontal Diseases / Q000382:microbiology / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*
D001425:Bacterial Outer Membrane Proteins