Isolation and characterisation of 3-deoxy-D-manno-2-octulopyranosonate 7-(2-aminoethyl phosphate) from the inner core region of Escherichia coli K-12 and Salmonella minnesota lipopolysaccharides.
The title compound (PE-Kdo) was isolated after hydrolysis of the lipopolysaccharides of Escherichia coli K-12 strain W3100 and Salmonella minnesota strains R4 and R7, and the location of the 2-aminoethyl phosphate group at position 7 was established by 13C-n.m.r. spectroscopy. Derivatives of PE-Kdo were acetylated, silylated, and methylated in order to evaluate their usefulness for analysis by g.l.c.-m.s.
Holst
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O
Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, F.R.G.
Röhrscheidt-Andrzejewski
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E
Brade
H
H
D004926:Escherichia coli; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D012475:Salmonella; D013400:Sugar Acids / Q000737:chemistry / Q000302:isolation & purification*
D008070:Lipopolysaccharides; D013400:Sugar Acids; C067007:3-deoxy-2-octulopyranosonate 7-(2-aminoethyl phosphate)