title: Isolation and amino acid sequence of a novel 6.8-kDa mitochondrial proteolipid from beef heart. Use of FAB-MS for molecular mass determination.
abstract: We have isolated a 6.8 kDa proteolipid from an acidic chloroform/methanol extract of bovine cardiac muscle. The molecular mass of the polypeptide was measured by fast atom bombardment-mass spectrometry (FAB-MS) (m/z6834.1). Its amino acid sequence was partly determined by direct sequencing and completed by characterization of cyanogen bromide and tryptic fragments (sequencing, FAB-MS and amino acid analysis). The polypeptide consists of 60 amino acid residues. Polyclonal antibodies raised in rabbit allowed its localization by electroimmunoblotting in mitochondria.
authors.affiliation: Laboratoire de Chimie Organique des Substances Naturelles, URA CNRS 31, Strasbourg, France.
authors.forename: E
authors.initials: E
authors.lastname: Terzi
authors.forename: P
authors.initials: P
authors.lastname: Boyot
authors.forename: A
authors.initials: A
authors.lastname: Van Dorsselaer
authors.forename: B
authors.initials: B
authors.lastname: Luu
authors.forename: E
authors.initials: E
authors.lastname: Trifilieff
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D015151:Immunoblotting; D013058:Mass Spectrometry; D008929:Mitochondria, Heart / Q000032:analysis*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000302:isolation & purification; D010455:Peptides / Q000302:isolation & purification; D011510:Proteolipids / Q000302:isolation & purification*; D012997:Solvents
chemical_list: D010446:Peptide Fragments; D010455:Peptides; D011510:Proteolipids; D012997:Solvents