Evidence for participation of the macrophage in Shiga-like toxin II-induced lethality in mice.
Seven strains of inbred mice were compared for their susceptibility to the lethal effects of Shiga-like toxin II (SLT II). A/J mice, which are unable to produce the C5 component of complement, did not differ from C5 normal mice in susceptibility to SLT II. CBA/NJ mice (hemizygous for X-linked immunodeficiency) did not differ from the B-cell sufficient CBA/J strain. C3H/HeJ mice, defective in macrophage response to lipopolysaccharide (Lpsd), showed a consistently and significantly longer mean time to death than did the normally responsive C3H/HeN strain. C57BL/10ScN mice, which also carry the Lpsd allele, showed a similar but smaller difference in mean time to death compared with the C57BL/10SnJ strain. Production of tumor necrosis factor could be induced in vitro by SLT II treatment of C3H/HeN, but not C3H/HeJ macrophages. These results imply that antibody and complement production do not modulate SLT II lethality in mice, but that the macrophage may contribute to SLT II-induced injury.
Barrett
T J
TJ
Division of Bacterial Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
Potter
M E
ME
Strockbine
N A
NA
D000483:Alleles; D000818:Animals; D001427:Bacterial Toxins / Q000633:toxicity*; D003182:Complement C5 / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008805:Mice, Inbred A; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D022641:Shiga Toxin 2; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D001427:Bacterial Toxins; D003182:Complement C5; D008070:Lipopolysaccharides; D022641:Shiga Toxin 2; D014409:Tumor Necrosis Factor-alpha; D003907:Dexamethasone