Determination of temafloxacin, sarafloxacin, and difloxacin in bulk drug and dosage forms by high-performance liquid chromatography.
The fluoroquinolones, temafloxacin, sarafloxacin, and difloxacin, are determined in the bulk drug substances and in a variety of dosage form using high-performance liquid chromatography (HPLC). The HPLC system used is also applicable for ciprofloxacin and norfloxacin. The procedure uses UV detection at 280 nm, which provides a linear response of the subject compounds to at least 20 micrograms/ml. Assay precision (RSD) values were +/- 1.2% or better for the bulk drugs and ranged from +/- 0.42 to +/- 2.3% for suspension, capsule, and tablet formulations. Drug recoveries were quantitative from the dosage forms tested. Sensitivity of the subject compounds is approximately 50 ng/ml (2.5 ng on column).
Bauer
J F
JF
Analytical Research Department, Abbott Laboratories, North Chicago, Illinois 60064-4000.
Elrod
L
L
Fornnarino
J R
JR
Heathcote
D E
DE
Krogh
S K
SK
Linton
C L
CL
Norris
B J
BJ
Quick
J E
JE
D000890:Anti-Infective Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002939:Ciprofloxacin / Q000009:adverse effects / Q000031:analogs & derivatives / Q000032:analysis; D024841:Fluoroquinolones; D015363:Quinolones; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D015363:Quinolones; C054745:temafloxacin; D002939:Ciprofloxacin; C047225:difloxacin; C047224:sarafloxacin