Competition between transcription complex assembly and chromatin assembly on replicating DNA.
We have used a Xenopus egg extract to show that a competition exists between the assembly of transcription complexes and nucleosomes on replicating 5S DNA. This competition results in the establishment of a transcriptionally repressed state for 5S DNA that is dependent on core histones but not on the precise positioning of the cores. The repression is selective, since satellite I DNA is not significantly repressed under these conditions. We demonstrate that the efficiency of chromatin assembly compared with transcription complex assembly is an important variable in determining gene activity.
Almouzni
G
G
Laboratory of Molecular Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892.
Méchali
M
M
Wolffe
A P
AP
D000818:Animals; D002645:Chickens; D002843:Chromatin / Q000502:physiology* / Q000648:ultrastructure; D004247:DNA / Q000235:genetics; D004261:DNA Replication; D004276:DNA, Satellite / Q000235:genetics; D004277:DNA, Single-Stranded / Q000235:genetics; D004278:DNA, Superhelical / Q000235:genetics; D003850:Deoxyribonuclease I; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D006657:Histones / Q000097:blood; D007700:Kinetics; D008836:Micrococcal Nuclease; D008957:Models, Genetic; D009865:Oocytes / Q000502:physiology; D013698:Templates, Genetic; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D014982:Xenopus laevis
D002843:Chromatin; D004276:DNA, Satellite; D004277:DNA, Single-Stranded; D004278:DNA, Superhelical; D006657:Histones; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors; D004247:DNA; D003850:Deoxyribonuclease I; D008836:Micrococcal Nuclease