title: Synthesis of thiomorpholino buffers for isoelectric focusing in immobilized pH gradients.
abstract: The two commercially available Immobilines having a pK of 6.2 (2-morpholino ethyl acrylamide) and 7.0 (3-morpholinopropylacrylamide) have been modified and two new buffers have been synthesized: 2-thiomorpholinoethylacrylamide, pK 6.6, and 3-thiomorpholinopropyl acrylamide, pK 7.4. The replacement of an oxygen with a sulfur atom in the morpholino ring is thus seen to shift the pK values of these two bases by +0.4 pH units. In formulations in which the two new bases replaced the standard morpholino derivatives, identical pH profiles and protein patterns were obtained. The reason for this work was to try to close the gap between the pK 7.0 and 8.5 species and to provide the users of immobilized pH gradients with more buffers in the neutral pH region. The two new thiomorpholino derivatives are an important step in this direction.
authors.affiliation: Department of Biomedical Sciences and Technologies, University of Milano, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Chiari
authors.forename: P G
authors.initials: PG
authors.lastname: Righetti
authors.forename: P
authors.initials: P
authors.lastname: Ferraboschi
authors.forename: T
authors.initials: T
authors.lastname: Jain
authors.forename: R
authors.initials: R
authors.lastname: Shorr
mesh_terms: D000178:Acrylamides / Q000138:chemical synthesis*; D002021:Buffers; D002855:Chromatography, Thin Layer; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D009682:Magnetic Resonance Spectroscopy; D009025:Morpholines / Q000138:chemical synthesis*
chemical_list: D000178:Acrylamides; D002021:Buffers; D009025:Morpholines; C067531:2-thiomorpholinoethylacrylamide; C067532:3-thiomorpholinopropylacrylamide