title: Phosphorylation of the skeletal muscle AMP-deaminase by protein kinase C.
abstract: Protein kinase C catalyzes phosphorylation of the rat skeletal muscle AMP-deaminase in the presence of calcium ions and phosphatidylserine. At the same time, the catalytic subunit of cAMP-dependent protein kinase fails to phosphorylate AMP-deaminase. Ca2+, phosphatidylserine-dependent phosphorylation decreases three-fold (from 0.6 to 0.2 mM) the Km value and does not affect Vmax. Protein kinase C-induced phosphorylation of AMP-deaminase, besides ADP-ribosylation, is suggested to be involved in regulating the AMP-deaminase activity in vivo.
authors.affiliation: Institute of Biochemistry, Academy of Sciences of Arm, SSR, Yerevan.
authors.forename: E K
authors.initials: EK
authors.lastname: Tovmasian
authors.forename: R L
authors.initials: RL
authors.lastname: Hairapetian
authors.forename: E V
authors.initials: EV
authors.lastname: Bykova
authors.forename: S E
authors.initials: SE
authors.lastname: Severin
authors.forename: A V
authors.initials: AV
authors.lastname: Haroutunian
mesh_terms: D000659:AMP Deaminase / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology*; D006801:Humans; D007700:Kinetics; D008970:Molecular Weight; D009132:Muscles / Q000201:enzymology*; D009709:Nucleotide Deaminases / Q000378:metabolism*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats
chemical_list: D011493:Protein Kinase C; D009709:Nucleotide Deaminases; D000659:AMP Deaminase