Isolation and characterization of a repetitive DNA element from the genome of the human filarial parasite, Brugia malayi.
The genome of the human filarial parasite Brugia malayi contains at least two major repetitive DNA elements. One, referred to as the HhaI family, consists of 10(4)-10(5) tandemly arrayed copies per haploid genome of a monomer of 322 base pairs and does not contain a cleavage site for the restriction endonuclease MboI. We constructed a library of MboI-digested genomic B. malayi DNA in BamHI-cut M13mp18 resulting in the exclusion of the HhaI repeat family from the library. Hybridization of this genomic library with nick-translated genomic DNA yielded several copies of a repeat family which we have named the BmMboI family. From sequence analysis of more than 50 monomers, which differ from each other in sequence and length, we have been able to divide the monomers into several regions based on the level of sequence conservation. Southern blot analyses of B. malayi genomic DNA digested with a variety of restriction endonucleases and probed with the isolated repeat demonstrate multiple bands of varying sizes except with HindIII-cut DNA, where the repeat is found only in very high-molecular-weight DNA.
Natarajan
S
S
Department of Pathology, University of Connecticut Health Center, Farmington.
Werner
C
C
Cameron
M
M
Rajan
T V
TV
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002009:Brugia / Q000235:genetics*; D016384:Consensus Sequence; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; D006238:Haploidy; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid
D004247:DNA; D015246:Deoxyribonuclease EcoRI; D015252:Deoxyribonucleases, Type II Site-Specific; C024405:GATC-specific type II deoxyribonucleases