Purification and characterization of two types of alkaline serine proteases produced by an alkalophilic actinomycete.
Two types of alkaline serine proteases were isolated from the culture filtrate of an alkalophilic actinomycete, Nocardiopsis dassonvillei OPC-210. The enzymes (protease I and protease II) were purified by acetone precipitation, DEAE-Sephadex A-50, CM-Sepharose CL-6B, Sephadex G-75 and phenyl-Toyopearl 650 M column chromatography. The purified enzymes showed a single band on sodium dodecyl sulphate polyacrylamide gel electrophoresis. The molecular weights of proteases I and II were 21,000 and 36,000, respectively. The pIs were 6.4 (protease I) and 3.8 (protease II). The optimum pH levels for the activity of two proteases were pH 10-12 (protease I) and pH 10.5 (protease II). The optimum temperture for the activity of protease I was 70 degrees C and that for protease II was 60 degrees C. Protease I was stable in the range of pH 4.0-8.0 up to 60 degrees C and protease II was stable in the range of pH 6.0-12.0 up to 50 degrees C.
Tsujibo
H
H
Osaka University of Pharmaceutical Sciences, Matsubara, Japan.
Miyamoto
K
K
Hasegawa
T
T
Inamori
Y
Y
D000192:Actinomycetales / Q000145:classification / Q000201:enzymology*; D002413:Cations, Divalent / Q000494:pharmacology; D002614:Chelating Agents / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D008970:Molecular Weight; D012697:Serine Endopeptidases / Q000302:isolation & purification* / Q000378:metabolism; D013696:Temperature
D002413:Cations, Divalent; D002614:Chelating Agents; D012697:Serine Endopeptidases; C032923:microbial serine proteinases