Properties and possible function of phosphatidylinositol-transfer proteins.
It is proposed that the phosphatidylinositol-transfer protein (PI-TP) may function as a carrier of phosphatidylinositol (PI) in the cell. PI-TP occurs in all mammalian tissues examined and appears to be strongly conserved. Its intracellular distribution was studied by immunoblotting and immunofluorescence techniques. PI-TP displays a dual specificity in that it preferentially transfers PI over phosphatidylcholine (PC) between membranes. Its lipid binding site and transfer characteristics were investigated with fluorescent PI and PC analogues containing parinaroyl- and pyrenylacyl-labeled chains. PI-TP is ideally suited for maintaining PI levels in intracellular membranes, possibly the plasma membrane.
Wirtz
K W
KW
Center for Biomembranes and Lipid Enzymology, State University of Utrecht, The Netherlands.
Snoek
G T
GT
Gadella
T W
TW
van Paridon
P A
PA
Somerharju
P J
PJ
D000818:Animals; D001665:Binding Sites / Q000502:physiology; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D008565:Membrane Proteins; D010716:Phosphatidylinositols / Q000378:metabolism*; D049294:Phospholipid Transfer Proteins; D029701:Saccharomyces cerevisiae Proteins
D002352:Carrier Proteins; D008565:Membrane Proteins; D010716:Phosphatidylinositols; D049294:Phospholipid Transfer Proteins; C118289:SEC24 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins