title: False negative results in anti-HIV ELISA due to insufficient antigen coating of microtitre plates.
abstract: Since routine ELISA screening of blood donors for anti-HIV antibodies was introduced, much attention has been given to the specificity and the sensitivity of this assay. Most papers deal with false positive reactions while only a few have taken account of false negative results. We have found that insufficient HIV-antigen coating of the microtitre plates can lead to false negative results. It is essential that the producers of the ELISA test kits use control systems which guarantee sufficient antigen coating.
authors.affiliation: Department of Clinical Immunology and Blood Bank, Copenhagen University, Hvidovre Hospital, Denmark.
authors.forename: J
authors.initials: J
authors.lastname: Rosenkvist
authors.forename: E
authors.initials: E
authors.lastname: Dybkjaer
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005188:False Negative Reactions; D015483:HIV Antibodies / Q000032:analysis*; D015488:HIV Antigens; D006801:Humans
chemical_list: D015483:HIV Antibodies; D015488:HIV Antigens