Criteria for the selection of a solid phase to be used in immunoassays.
Heterogeneous immunoassays are very sensitive and only limited in terms of performance by non specific binding. They require separation of free from bound fractions and concomitant use of a solid phase coated with an immunoreactive component (i.e. immunosorbent). The improvement of these key immunosorbents is crucial and involves a great deal of expertise and capabilities. Specifications differ according to procedure (e.g. capture or competitive assay). Each routinely used solid phase, such as polystyrene wells, porous membrane or dispersible microbeads, presents specific performance characteristics, advantages, and drawbacks. Among the tasks to be implemented are optimization of the spatial orientation of immunological reagents, selection of the surface neutral hydrophilic support, acceleration of reactions by increasing the reactive surface area of the supports, streamlining and simplification of procedural steps. These various aspects are abundantly described and emphasized here.
Delagneau
J F
JF
Diagnostics Pasteur, Marnes-La-Coquette.
Masseyeff
R
R
D020106:Acrylamide; D000178:Acrylamides; D002482:Cellulose; D007118:Immunoassay / Q000379:methods*; D007202:Indicators and Reagents; D011137:Polystyrenes; D012685:Sepharose
D000178:Acrylamides; D007202:Indicators and Reagents; D011137:Polystyrenes; D020106:Acrylamide; D002482:Cellulose; D012685:Sepharose