title: The cloning and expression of a gene encoding haemolytic activity from the fish pathogen Renibacterium salmoninarum.
abstract: A gene encoding haemolytic activity from Renibacterium salmoninarum (strain PPD) was cloned into Escherichia coli using the cosmid vector pHC79, and subsequently subcloned on a 1.6 kbp SAlI fragment into pBR328. Southern blot hybridisation revealed that a homologous sequence is found in other strains of R. salmoninarum.
authors.affiliation: Department of Biological Sciences, Polytechnic South West, Plymouth, U.K.
authors.forename: A J
authors.initials: AJ
authors.lastname: Evenden
authors.forename: M L
authors.initials: ML
authors.lastname: Gilpin
authors.forename: C B
authors.initials: CB
authors.lastname: Munn
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D003001:Cloning, Molecular; D003360:Cosmids; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D006093:Gram-Positive Asporogenous Rods / Q000235:genetics* / Q000472:pathogenicity; D006460:Hemolysin Proteins / Q000235:genetics*; D006461:Hemolysis; D015183:Restriction Mapping; D012487:Salmonidae / Q000382:microbiology; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004269:DNA, Bacterial; D006460:Hemolysin Proteins