title: Characterization of the platelet aggregation inducer and inhibitor isolated from Crocus sativus.
abstract: Bulbs of Crocus sativus variety Cartwrightianus were found to contain both a platelet aggregation inducer and inhibitor. The aggregating factor has a Mr of 42 kDa estimated by a Sephadex G75 column and SDS-polyacrylamide slab gel electrophoresis. It was found to lack enzymatic activity such as proteinase, esterase and acid or alkaline phosphatase. The inhibitory factor was also purified to homogeneity by different chromatographic techniques and shows a Mr of 27 kDa as it was estimated by Biogel P30 column and SDS-polyacrylamide slab gel electrophoresis. It was found to possess strong proteinase activity.
authors.affiliation: Dept. of Chemical Enginnering, Aristotelian University of Thessaloniki, Greece.
authors.forename: M
authors.initials: M
authors.lastname: Liakopoulou-Kyriakides
authors.forename: A I
authors.initials: AI
authors.lastname: Skubas
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism; D004950:Esterases / Q000378:metabolism; D008970:Molecular Weight; D010940:Plant Proteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D010974:Platelet Aggregation; D010975:Platelet Aggregation Inhibitors / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D010940:Plant Proteins; D010975:Platelet Aggregation Inhibitors; D004950:Esterases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D010450:Endopeptidases