Standardization of serum fructosamine assays.
We have calibrated a secondary serum protein standard by use (as primary standards) of samples of albumin and polylysine glycated with [14C]glucose in vitro, the glycation of which was assessed by radioactivity measurements and by elementary analysis for C and N. Using this standard for calibration in our improved fructosamine assay, one obtains an average fructosamine value of 247 mumol/L for nondiabetic individuals (or, in terms of total serum protein, 3.2 mumol/g)--about a tenth the value we obtained when we used the fructosamine assay of Johnson et al. (Clin Chim Acta 1983;127:87-95), standardized with desoxymorpholinofructose. In contrast, results corresponded well with the value for mean glycation of serum proteins, 3 mumol/g, determined by a furosine/HPLC method. Evidently the proposed procedure, in which a standard sharing the binding characteristics of endogenous glycated proteins is used together with our modified new fructosamine assay, leads to more realistic values for the concentrations of glycated serum proteins.
Schleicher
E D
ED
Diabetes Research Group, Institute for Clinical Chemistry, Munich, F.R.G.
Vogt
B W
BW
D002244:Carbon / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D019270:Fructosamine; D005947:Glucose / Q000032:analysis; D017127:Glycation End Products, Advanced; D006595:Hexosamines / Q000097:blood* / Q000592:standards; D006801:Humans; D008433:Mathematics; D009584:Nitrogen / Q000032:analysis; D011107:Polylysine / Q000032:analysis; D012709:Serum Albumin / Q000032:analysis / Q000592:standards; D000093423:Glycated Serum Albumin
D017127:Glycation End Products, Advanced; D006595:Hexosamines; D012709:Serum Albumin; D011107:Polylysine; D019270:Fructosamine; D002244:Carbon; D005947:Glucose; D009584:Nitrogen; D000093423:Glycated Serum Albumin