Histochemical response of mice to mistletoe lectin I (ML I).
The acute toxicity of lectin ML I from the toxic drug, mistletoe, was demonstrated in previous experiments. Because the reason for this extremely high toxicity is not yet clear, mice were studied histochemically at different times after treatment with various doses of ML I, ML I A or ML I B chain separately, or recombinations of ML I A and ML I B. Various plasma membrane-associated hydrolases as well as Golgi apparatus-and endoplasmic reticulum-linked hydrolases, peroxisomal and extraperoxisomal oxidases, lysosomal hydrolases, mitochondrial dehydrogenases, the cytoskeletal proteins keratin and vimentin as well as iron, glycogen and lipids were analysed in all organs and tissues of female mice. Irrespective of the dose, a clear-cut response was only observed in the liver. After ML I treatment, glycogen disappeared completely from all hepatocytes, and this effect did not depend on the ML I-concentration and exposure time. The increase in activity of Golgi-associated thiamine pyrophosphatase in hepatocytes and of non-specific alkaline phosphatase in the sinusoidal endothelial cells depended on the applied ML I concentration and the time of treatment. Doses of 600 or 900 ng ML I/kg drastically increased the phosphatase activities. These clear-cut changes of glycogen and enzyme activities were not observed after administration of the ML I B chain alone, and less so when the mice were treated only with the ML I A chain, or were treated with a recombination of ML I A and ML I B even at concentrations higher than that of ML I.(ABSTRACT TRUNCATED AT 250 WORDS)
Gossrau
R
R
Institut für Anatomie, Freien Universität Berlin, Germany.
Franz
H
H
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004798:Enzymes / Q000378:metabolism; D005260:Female; D006651:Histocytochemistry; D007274:Injections, Intraperitoneal; D008099:Liver / Q000166:cytology / Q000201:enzymology; D008112:Liver Glycogen / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains / Q000378:metabolism*; D028321:Plant Preparations; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D013834:Thiamine Pyrophosphatase / Q000378:metabolism; D013997:Time Factors; D014118:Toxins, Biological / Q000008:administration & dosage / Q000378:metabolism* / Q000633:toxicity
D004798:Enzymes; D008112:Liver Glycogen; D028321:Plant Preparations; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; C488862:mistletoe lectin I; C009674:ribosome inactivating protein, Viscum; D000469:Alkaline Phosphatase; D013834:Thiamine Pyrophosphatase