A novel 40-kDa protein induced by heat shock and other stresses in mammalian and avian cells.
When HeLa cells were exposed to heat shock or other stresses, a 40-kDa protein (p40) was induced in addition to the classical heat shock proteins with molecular sizes of 110-, 90-, 70-, and 47-kDa. The p40 was induced not only by heat shock but by arsenite and 2-azetidine carboxylic acid. Also, it was induced in rat, mouse and chick cells by these stresses. The p40 was a basic protein (pI divided by 9.2) as judged by two-dimensional gel electrophoresis. To our knowledge, p40 is a novel heat shock protein in mammalian and avian cells.
Ohtsuka
K
K
Department of Experimental Radiology, Aichi Cancer Center Research Institute, Nagoya, Japan.
Masuda
A
A
Nakai
A
A
Nagata
K
K
D000818:Animals; D001151:Arsenic / Q000494:pharmacology; D018053:Arsenites; D002478:Cells, Cultured; D002642:Chick Embryo; D003609:Dactinomycin / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional; D005786:Gene Expression Regulation / Q000187:drug effects; D006367:HeLa Cells; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D007526:Isoelectric Point; D051379:Mice; D008970:Molecular Weight; D051381:Rats
D018053:Arsenites; D006360:Heat-Shock Proteins; D003609:Dactinomycin; C015001:arsenite; D001151:Arsenic