Manipulation of cell cycle kinetics: influence on the cytotoxicity of doxorubicin in human breast cancer cells.
In vitro exposure of estrogen receptor-negative (ER-) EVSA-T human breast cancer cells to insulin and/or estradiol had no effect on cell cycle distribution, in contrast to a 3-5-fold increase in the percentages of cells in the S-phase of the cell cycle in the ER+ MCF-7 cell line. Estrogen pretreatment of MCF-7 cells followed by incubation with doxorubicin resulted in an augmented inhibition of cell growth compared to unstimulated controls. This delay in growth was accompanied by a decrease in the percentages of cells actively synthesizing DNA, and by an augmented percentage of cells exhibiting a G2M-amount of DNA at the end of a 6-9 day period of culture in complete growth medium.
Bontenbal
M
M
Division of Endocrine Oncology (Department of Medical Oncology), Dr Daniel den Hoed Cancer Center, Rotterdam, The Netherlands.
Sieuwerts
A M
AM
Peters
H A
HA
Sonneveld
P
P
Foekens
J A
JA
Klijn
J G
JG
D001943:Breast Neoplasms / Q000188:drug therapy*; D001973:Bromodeoxyuridine; D002453:Cell Cycle / Q000187:drug effects / Q000235:genetics; D004247:DNA / Q000378:metabolism; D004317:Doxorubicin / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D007700:Kinetics; D011960:Receptors, Estrogen / Q000187:drug effects; D014407:Tumor Cells, Cultured
D007328:Insulin; D011960:Receptors, Estrogen; D004958:Estradiol; D004317:Doxorubicin; D004247:DNA; D001973:Bromodeoxyuridine