Phenotypic modulation in sub-populations of human articular chondrocytes in vitro.
Human articular cartilage has been separated into surface (approx. 15% of tissue depth) and deep zones (remaining tissue) and the constituent chondrocytes released by enzymic digestion. Subsequent culture either as a low density monolayer or as a suspension over agarose revealed distinct morphological and synthetic behaviour in the two populations. Whilst in monolayer these morphological differences disappeared with time in culture, over agarose they remained. Surface zone cells formed two types of cell cluster; one that was highly cellular with little extracellular matrix, and the other less frequent, which formed copious amounts of fibrillar matrix. Both types of cluster were surrounded by a layer of flattened chondrocytes. In contrast, deep cells formed a single cluster type that lacked a surrounding cell layer, but formed large amounts of sparse cartilage-like matrix and comprised morphologically typical chondrocytes. In monolayer, both populations gradually ceased to synthesise cartilage matrix components with the exception of link protein. In suspension, whilst the chondrogenic phenotype per se was preserved, there was, nevertheless, a loss in qualitative synthetic heterogeneity, which exists between surface and deep cells, that was not accompanied by changes in the differential rate of 35S incorporation into proteoglycan. Under these conditions, surface cells that normally do not synthesise keratan sulphate initiated de novo synthesis of this glycosaminoglycan. Consequently, it appears that the observed modulation in synthetic ability of the cell sub-populations is independent of the cluster morphology, which, once established, remains constant throughout the culture period.
Archer
C W
CW
Department of Orthopaedic Surgery, University College and Middlesex School of Medicine, Stanmore, UK.
McDowell
J
J
Bayliss
M T
MT
Stephens
M D
MD
Bentley
G
G
D002358:Cartilage, Articular / Q000166:cytology* / Q000378:metabolism; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006801:Humans; D010641:Phenotype; D011509:Proteoglycans / Q000096:biosynthesis; D012685:Sepharose; D013431:Sulfates / Q000378:metabolism; D013462:Sulfur Radioisotopes
D011509:Proteoglycans; D013431:Sulfates; D013462:Sulfur Radioisotopes; D012685:Sepharose