Isolation of the perisinusoidal vitamin A-storing cells from rat liver on Ca2(+)-immobilized glass surfaces.
Calcium was immobilized on glass slides employing covalently coupled iminodiacetic acid. The calcium-coated glass surfaces were then used for purification and culturing of perisinusoidal vitamin A-storing cells from rat liver. The cells were isolated in a yield of 0.9 x 10(6) cells/g rat liver without cross-contamination by other hepatic cell types. The cells were characterized by morphology, vitamin A fluorescence, and immunohistochemistry to detect expression of cellular retinol-binding protein.
Mantovaara
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T
Institute of Biochemistry, University of Uppsala, Sweden.
Nordlinder
H
H
Pertoft
H
H
D000818:Animals; D002118:Calcium; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D005898:Glass; D007150:Immunohistochemistry; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014801:Vitamin A / Q000378:metabolism*
D014801:Vitamin A; D002118:Calcium