title: Inhibition of osteoclastic bone resorption by mechanical stimulation in vitro.
abstract: The influence of mechanical stimulation by intermittent compressive force (ICF) of physiologic magnitude on osteoclastic bone resorption was investigated in cultures of fetal mouse cartilaginous long bones. Exposure to ICF resulted in a significant decrease in mineral resorption, as indicated by the decreased release of 45Ca and a decreased number of osteoclasts in the diaphysis. Conditioned medium (CM) from ICF-exposed periosteum-free cultures (ICF-CM), but not from control cultures (Co-CM), inhibited mineral resorption in fresh bones cultured under control conditions. Co-CM increased, but ICF-CM decreased, the number of tartrate-resistant acid phosphatase-positive cells in 7-day bone marrow cultures. Direct exposure of bone marrow cultures to ICF yielded the same results. Thus, osteoclastic bone resorption in cartilaginous long bones is inhibited by ICF in vitro. A soluble factor(s) acting on tartrate-resistant acid phosphatase-positive, osteoclast precursor-like cells seems to play a role in this effect.
authors.affiliation: Department of Oral Cell Biology, ACTA-Vrije Universiteit, Amsterdam, The Netherland.
authors.forename: J
authors.initials: J
authors.lastname: Klein-Nulend
authors.forename: J P
authors.initials: JP
authors.lastname: Veldhuijzen
authors.forename: M E
authors.initials: ME
authors.lastname: van Strien
authors.forename: M
authors.initials: M
authors.lastname: de Jong
authors.forename: E H
authors.initials: EH
authors.lastname: Burger
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001862:Bone Resorption; D001842:Bone and Bones / Q000033:anatomy & histology / Q000166:cytology / Q000378:metabolism; D003470:Culture Media; D004351:Drug Resistance; D051379:Mice / Q000196:embryology; D008903:Minerals / Q000378:metabolism; D009924:Organ Culture Techniques; D010010:Osteoclasts / Q000502:physiology*; D010812:Physical Stimulation; D011312:Pressure; D013644:Tartrates / Q000494:pharmacology
chemical_list: D003470:Culture Media; D008903:Minerals; D013644:Tartrates; D000135:Acid Phosphatase; C029768:tartaric acid