title: Liquid chromatographic determination of three sulfonamides in animal tissue and egg.
abstract: Sulfonamides are widely used as a feed additive in animal production in Japan. The present paper is a determination of 3 sulfonamides: sulfamethazine (SMZ), sulfamonomethoxine [SMX, 4-amino-N-(3-methoxypyrazinyl)-benzenesulfonamide], and sulfadimethoxine (SDX) in animal tissue and egg by liquid chromatography (LC). Tissues were extracted with acetonitrile and fat was removed by liquid/liquid partition. The sulfonamides were purified by an ODS cartridge column; then each compound was separated by an ODS LC column and detected at 268 nm. Quantification levels were 0.02 ppm for SMZ and SMX, and 0.04 ppm for SDX; detection limits were 0.01 ppm for SMZ and SMX, and 0.02 ppm for SDX. Calibration curves were linear between 2 and 40 ng for SMZ and SMX, and between 4 and 80 ng for SDX. Recoveries from muscle and egg samples spiked with 1-2 micrograms/10 g were 81-98%.
authors.affiliation: Tokyo Metropolitan Research Laboratory of Public Health, Department of Food Hygiene and Nutrition, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Horii
authors.forename: C
authors.initials: C
authors.lastname: Momma
authors.forename: K
authors.initials: K
authors.lastname: Miyahara
authors.forename: T
authors.initials: T
authors.lastname: Maruyama
authors.forename: M
authors.initials: M
authors.lastname: Matsumoto
mesh_terms: D000818:Animals; D002417:Cattle; D002645:Chickens; D002853:Chromatography, Liquid; D004350:Drug Residues / Q000032:analysis*; D004531:Eggs / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D009132:Muscles / Q000737:chemistry; D013449:Sulfonamides / Q000032:analysis*; D013552:Swine
chemical_list: D013449:Sulfonamides