Influence of cytochrome P450 mixed-function oxidase induction on the acute toxicity to rainbow trout (Salmo gairdneri) of primary aromatic amines.
The influence of enzyme induction on the acute toxicity of aniline and 4-chloroaniline to rainbow trout (Salmo gairdneri) was investigated. For these two xenobiotics, bioactivation reactions are known to occur in mammals. Induction of cytochrome P450 mixed-function oxidase was obtained by intraperitoneal (ip) injection of trout with a mixture of polychlorinated biphenyls (Aroclor 1254). Five days after ip injection with three different doses of Aroclor 1254 (50, 100, and 200 mg/kg), benzo[a]pyrene hydroxylase activity in trout liver microsomes increased five- to sixfold. Cytochrome P450 concentrations in the microsomes were slightly, but significantly, enhanced in two of the three dose levels. The 96-hr LC50's of aniline and 4-chloroaniline were not affected by pretreatment with Aroclor 1254, suggesting that metabolic activation does not necessarily play a role in the acute toxicity of aromatic amines to fish.
Hermens
J L
JL
United States Environmental Protection Agency, Environmental Research Laboratory, Duluth, Minnesota 55804.
Bradbury
S P
SP
Broderius
S J
SJ
D000814:Aniline Compounds / Q000633:toxicity*; D000818:Animals; D001140:Aroclors / Q000494:pharmacology; D001579:Benzopyrene Hydroxylase / Q000378:metabolism; D020111:Chlorodiphenyl (54% Chlorine); D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D004790:Enzyme Induction / Q000187:drug effects; D066298:In Vitro Techniques; D007928:Lethal Dose 50; D008862:Microsomes, Liver / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000096:biosynthesis*; D011506:Proteins / Q000378:metabolism; D014337:Trout / Q000502:physiology*
D000814:Aniline Compounds; D001140:Aroclors; D011506:Proteins; D020111:Chlorodiphenyl (54% Chlorine); D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D001579:Benzopyrene Hydroxylase; C004658:4-chloroaniline