title: Cloning of two hypothalamic cDNAs encoding tissue-specific transcripts in the preoptic area and testis.
abstract: Neuropeptides are central to the regulation of normal sexual development and reproduction. Two new hypothalamic cDNAs have been identified by Northern blot analysis and molecular cloning. Each potentially encodes a precursor for a unique GnRH-like decapeptide. Northern blot analysis reveals tissue-specific transcripts from each gene in the hypothalamus and testis. These preoptic area regulatory factor genes, PORF-1 and PORF-2, may thus encode members of a family of GnRH-related peptides which have been described in these tissues.
authors.affiliation: Department of Molecular and Cell Biology, University of Connecticut, Storrs 06269-3125.
authors.forename: F V
authors.initials: FV
authors.lastname: Nowak
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D007987:Gonadotropin-Releasing Hormone / Q000235:genetics*; D007031:Hypothalamus / Q000737:chemistry*; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011301:Preoptic Area / Q000737:chemistry*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D012045:Regulatory Sequences, Nucleic Acid; D013737:Testis / Q000737:chemistry*; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D007987:Gonadotropin-Releasing Hormone; D004247:DNA