Protein analysis of newly isolated variants of echovirus type 18 by electrophoresis and western blotting.
The protein patterns of two newly isolated antigenic variants of echovirus type 18 were compared with those of the prototype strain. Electrophoretic mobilities of VP1 and VP2 of the variants differed from those of the prototype strain. The antiserum against the prototype strain reacted to VP0 and VP2 in the Western blotting experiment. In addition, the variants differed from the prototype strain in growth behavior in the cultured cells.
Hayase
Y
Y
Department of Virology, Jichi Medical School, Tochigi-Ken, Japan.
Toya
K
K
Tanaka
T
T
Tobita
K
K
D015153:Blotting, Western; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D029822:Enterovirus B, Human / Q000032:analysis*; D006801:Humans; D009500:Neutralization Tests; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000032:analysis*
D014764:Viral Proteins