Immunohistochemical localization of 14 kDa beta-galactoside-binding lectin in various organs of rat.
Immunohistochemical localization of 14 kDa beta-galactoside-binding lectin in various organs of adult rat was achieved using a monospecific antibody raised against lectin purified from rat lung. The antibody-stained cells were formed into small aggregates, thin fascicles, or thick bundles in the walls of blood vessels, gastrointestinal tracts and urogenital organs. From the patterns of distribution, as well as their organization, these immunoreactive cells were regarded as smooth muscle cells. This was confirmed by a double immunofluorescence study using a mixture of anti 14 kDa lectin and anti alpha-smooth muscle-specific actin antibodies. Strong 14 kDa lectin immunoreactivity was seen in the pericellular matrix of smooth muscle cells in intact organs as well as in detergent-treated organs from which all cellular components were extracted. From these findings, it is suggested that the 14 kDa lectin may be externalized by smooth muscle cells into their pericellular matrix and participate in the crosslinking of the complementary glycoconjugate(s) localized at that site. The macromolecular xomplex of glycoconjugates thus formed around smooth muscle cells may play a role in anchoring smooth muscle cells to the pericellular connective tissue thereby permitting the force of muscle contraction to be efficiently transmitted to the surrounding connective tissue proper.
Wasano
K
K
Department of Anatomy, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Hirakawa
Y
Y
Yamamoto
T
T
D000818:Animals; D000911:Antibodies, Monoclonal; D001808:Blood Vessels / Q000166:cytology / Q000378:metabolism*; D005697:Galactosides / Q000378:metabolism*; D006027:Glycosides / Q000378:metabolism*; D007150:Immunohistochemistry; D037102:Lectins / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D009130:Muscle, Smooth / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D005697:Galactosides; D006027:Glycosides; D037102:Lectins