Protein kinase C-gamma is present in adriamycin resistant HL-60 leukemia cells.
The isoform pattern of protein kinase C (PKC) was examined in wild-type and Adriamycin-resistant (HL-60/AR) HL-60 leukemia cells. Analyses were carried out by immunoblotting with mouse monoclonal antibodies against PKC-alpha and PKC-beta and a rabbit polyclonal antibody against the variable (V3) region of PKC-gamma. HL-60/AR cells contained an equivalent level of PKC-alpha and a lower amount of PKC-beta than HL-60 cells. In contrast, only HL-60/AR cells contained PKC-gamma. These results indicate that the regulation of this family of isoenzymes is altered in drug-resistant cells.
Aquino
A
A
Laboratory of Biological Chemistry, National Cancer Institute, Bethesda, Maryland 20892.
Warren
B S
BS
Omichinski
J
J
Hartman
K D
KD
Glazer
R I
RI
D000918:Antibody Specificity; D015153:Blotting, Western; D004351:Drug Resistance; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D007951:Leukemia, Myeloid / Q000201:enzymology*; D011493:Protein Kinase C / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D007527:Isoenzymes; D011493:Protein Kinase C