The development and characterization of encephalitogenic cloned T cells specific for myelin proteolipid protein.
T-cell clones have been isolated from SJL/J mice after immunization with myelin proteolipid protein (PLP). The cloned cells responded strongly to PLP stimulation in vitro as well as to the synthetic PLP-related peptide 139-151. The response to PLP is Ia mediated, since it was inhibited by monoclonal antibodies to the matched I-As haplotype, but not with antibodies to other I-A haplotypes. Phenotypic analysis using immunofluorescence demonstrated the following characteristics of the clones: Thy-1+, CD4+, CD5+ and CD8-. Injection of 10-30 million PLP-activated cells from one clone induced severe experimental allergic encephalomyelitis in five mice, both clinically and histologically. This represents to our knowledge the first report of PLP-specific encephalitogenic cloned T cells.
van der Veen
R C
RC
Department of Neurology, Washington University School of Medicine, St. Louis, MO 63110.
Trotter
J L
JL
Hickey
W F
WF
Kapp
J A
JA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002455:Cell Division; D002490:Central Nervous System / Q000473:pathology; D002999:Clone Cells; D004660:Encephalitis / Q000276:immunology* / Q000473:pathology; D005260:Female; D006239:Haplotypes; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007116:Immunization, Passive; D051379:Mice; D008815:Mice, Inbred Strains; D009185:Myelin Proteins / Q000276:immunology*; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; D009185:Myelin Proteins