Estrogen regulation of creatine kinase-B in the rat uterus.
Creatine kinase-B (CKB) synthesis is rapidly and specifically induced by estrogen in the uterus of the immature rat. This study indicates that this elevation is due at least in part to increases in the levels of mRNA for CKB. The stimulation of CKB mRNA levels is rapid (a 7- to 10-fold increase is detected 1-3 h after estrogen administration), but transient, as levels return to near control values by 6 h. Analysis of cDNAs to both uterine and brain CKB mRNA indicate that the same sequence is expressed in both tissues despite earlier observations of heterogeneity of the protein isolated from the two tissues. A 1.7-kilobasepair DNA fragment containing the CKB promoter and 5' flanking sequences confers estrogen sensitivity on expression of the bacterial chloramphenicol acetyl transferase gene in HeLa cells on cotransfection with an estrogen-receptor expression vector. However, the CKB promoter sequences lack any motif with convincing similarity to the currently accepted consensus estrogen response element GGTCAnnnTGACC.
Pentecost
B T
BT
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.
Mattheiss
L
L
Dickerman
H W
HW
Kumar
S A
SA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase / Q000378:metabolism; D003402:Creatine Kinase / Q000096:biosynthesis / Q000235:genetics*; D004247:DNA / Q000737:chemistry; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation; D007527:Isoenzymes; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011960:Receptors, Estrogen / Q000187:drug effects / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D014599:Uterus / Q000187:drug effects / Q000201:enzymology*
D007527:Isoenzymes; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D004958:Estradiol; D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase; D003402:Creatine Kinase