[Directed reconstruction of the influenza virus hemagglutinin gene].
A new approach to create chimeric genes by directed exchange of oligonucleotide fragments was developed. By oligonucleotide-directed mutagenesis a few deletion mutants of the influenza virus hemagglutinin (HA) gene were obtained. These variants of HA gene contain unique restriction sites in DNA regions coding for the A and B epitopes of the HA molecule. The obtained special vectors may be used for cloning DNA fragments coding for new amino acid sequences in internal sites of the HA gene.
Petrenko
V A
VA
Kipriianov
S M
SM
Semenova
L N
LN
Boldyrev
A N
AN
Pozdniakov
P I
PI
Eroshkin
A M
AM
Sivolobova
G F
GF
D001483:Base Sequence; D002678:Chimera; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006389:Hemagglutinins, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009975:Orthomyxoviridae / Q000378:metabolism*; D015183:Restriction Mapping
D004274:DNA, Recombinant; D006389:Hemagglutinins, Viral
Napravlennaia rekonstruktsiia gena gemaggliutinina virusa grippa.