title: Increased frequency of 6-thioguanine-resistant peripheral blood lymphocytes in Werner syndrome patients.
abstract: The frequency of spontaneous 6-thioguanine (TG)-resistant peripheral blood lymphocytes in five unrelated Werner syndrome (WS) patients was determined using an autoradiographic labeling assay. The average frequency of TG-resistant lymphocytes was eightfold higher in WS patients than in sex- and age-matched normal control donors. This finding and previous identification of increased spontaneous chromosomal rearrangements and deletions in WS cells or cell lines suggest that WS is a human genomic instability or mutator syndrome.
authors.affiliation: Department of Medicine and Geriatrics, Osaka University Medical School, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Fukuchi
authors.forename: K
authors.initials: K
authors.lastname: Tanaka
authors.forename: Y
authors.initials: Y
authors.lastname: Kumahara
authors.forename: K
authors.initials: K
authors.lastname: Marumo
authors.forename: M B
authors.initials: MB
authors.lastname: Pride
authors.forename: G M
authors.initials: GM
authors.lastname: Martin
authors.forename: R J
authors.initials: RJ
authors.lastname: Monnat
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D004351:Drug Resistance; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D013866:Thioguanine / Q000633:toxicity*; D014898:Werner Syndrome / Q000235:genetics*
chemical_list: D013866:Thioguanine