Effects of androgen, fibroblast growth factors or other various growth factors on growth of Shionogi carcinoma cells in a protein-free medium.
Shionogi carcinoma 115 (SC115) has been accepted for 20 years as an androgen-responsive mouse mammary tumor. We have recently established an androgen-dependent cloned cell line (SC-3) from a SC115 tumor. We found in the present study that the growth of SC-3 cells can be stimulated by 10(-8) M testosterone (up to 100-fold) even in a protein-free medium beginning from plating [Ham's F-12: Eagle's minimum essential medium (1:1, v/v)]. In the protein-free culture, the proliferation of SC-3 cells was also found to be stimulated by acidic or basic fibroblast growth factor (FGF) alone (up to 50-fold) among various growth factors examined such as FGFs, insulin, insulin-like growth factor (IGF)-I, IGF-II, nerve growth factor, platelet-derived growth factor, epidermal growth factor, transforming growth factor (TGF)-alpha and TGF-beta. The testosterone (10(-8) M)- or FGF (10 ng/ml)-induced growth of SC-3 cells was abolished only by TGF-beta (greater than or equal to 1 ng/ml) among various growth factors examined. We show for the first time in this study an androgen-dependent growth of cancer cells (SC-3) in a protein-free medium. In the protein-free medium, growth of SC-3 cells is also stimulated by FGFs, and the androgen- or FGF-induced growth is inhibited only by TGF-beta.
Tanaka
A
A
Department of Pathology, Osaka University Medical School, Japan.
Lu
J
J
Yamanishi
H
H
Nonomura
N
N
Yasui
T
T
Maeyama
M
M
Nishizawa
Y
Y
Matsumoto
K
K
Sato
B
B
D000818:Animals; D002277:Carcinoma; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002999:Clone Cells; D003470:Culture Media; D004261:DNA Replication / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D006133:Growth Substances / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D007335:Insulin-Like Growth Factor II / Q000494:pharmacology; D007700:Kinetics; D008325:Mammary Neoplasms, Experimental; D051379:Mice; D009414:Nerve Growth Factors / Q000494:pharmacology; D013739:Testosterone / Q000494:pharmacology*; D016211:Transforming Growth Factor alpha / Q000494:pharmacology
D003470:Culture Media; D006133:Growth Substances; D007328:Insulin; D009414:Nerve Growth Factors; D016211:Transforming Growth Factor alpha; D013739:Testosterone; D005346:Fibroblast Growth Factors; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II