title: Biochemical properties of a P2Y-purinergic receptor.
abstract: The turkey erythrocyte has substantial value as a model for the study of a receptor that exhibits pharmacological properties very similar to those delineated in mammalian tissues for a P2Y-purinergic receptor. The G protein-dependent coupling of this receptor to phospholipase C can be studied in detail, and the availability of an abundant source of homogeneous cells from which highly purified plasma membranes can be prepared, has led to the development of a radiolabeled, reversibly binding radioligand for a P2Y-purinergic receptor and a photoaffinity covalent radiolabel for this receptor. This source of plasma membranes highly enriched in P2Y-purinergic receptors should also serve as a rich starting material for the eventual purification and structural characterization of this important signaling protein.
authors.affiliation: Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill 27599.
authors.forename: T K
authors.initials: TK
authors.lastname: Harden
authors.forename: J L
authors.initials: JL
authors.lastname: Boyer
authors.forename: H A
authors.initials: HA
authors.lastname: Brown
authors.forename: C L
authors.initials: CL
authors.lastname: Cooper
authors.forename: R A
authors.initials: RA
authors.lastname: Jeffs
authors.forename: M W
authors.initials: MW
authors.lastname: Martin
mesh_terms: D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000818:Animals; D004912:Erythrocytes / Q000378:metabolism*; D007700:Kinetics; D011983:Receptors, Purinergic / Q000187:drug effects / Q000378:metabolism*; D014422:Turkeys
chemical_list: D011983:Receptors, Purinergic; D000255:Adenosine Triphosphate