Separation of glycoprotein-N-glycans by high-pH anion-exchange chromatography.
A series of high-mannose and complex type glycoprotein-N-glycans was subjected to high-pH anion-exchange chromatography. The results revealed that this method represents a useful tool for analytical characterization of single oligosaccharides as well as preparative separation of complex mixtures of carbohydrate side-chains. On the other hand, it became evident that in several cases a combination of different chromatographic techniques is required for efficient separation of individual oligosaccharide species.
Pfeiffer
G
G
Biochemisches Institut am Klinikum, Universität Giessen, FRG.
Geyer
H
H
Geyer
R
R
Kalsner
I
I
Wendorf
P
P
D002852:Chromatography, Ion Exchange; D006023:Glycoproteins / Q000302:isolation & purification*; D006596:Hexosaminidases; D006863:Hydrogen-Ion Concentration; D008745:Methylation; D009844:Oligosaccharides / Q000302:isolation & purification; D011134:Polysaccharides / Q000302:isolation & purification*
D006023:Glycoproteins; D009844:Oligosaccharides; D011134:Polysaccharides; D006596:Hexosaminidases