Identification of a low molecular weight form of epithelial transforming growth factor.
We have purified a novel form of epithelial transforming growth factor (TGFe) from bovine kidney. Acid ethanol extracts of kidney were fractionated by size exclusion, reverse phase and cation exchange chromatography and activity was monitored by measuring growth of SW13 adrenocortical carcinoma cells in soft agar. The purified material was highly cationic, bound weakly to heparin and gave a band at 13-15000 Mr by SDS-PAGE following Bolton-Hunter iodination. This band correspond to the migration of biological activity extractable from gel slices. The results suggest that we have isolated a truncated form of TGFe which nonetheless retains biological activity.
Dunnington
D
D
Smithkline Beecham Pharmaceuticals, Department of Cell Sciences, King of Prussia, PA 19406-2799.
Prichett
W
W
Moyer
M
M
Greig
R
R
D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004848:Epithelium / Q000737:chemistry; D005346:Fibroblast Growth Factors / Q000378:metabolism; D006493:Heparin; D007668:Kidney / Q000737:chemistry*; D008970:Molecular Weight; D015291:Transforming Growth Factors / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
D005346:Fibroblast Growth Factors; D015291:Transforming Growth Factors; D006493:Heparin