Carbon filaments provide support and directionality to growing rat fetal spinal cord explants.
Spinal cord explants obtained from 15 to 17-day-old fetal rats were cultured on bundles of 5-7 microns diameter carbon filaments attached to the bottom of Petri dishes. After a 3 week incubation period, the cultures were fixed and observed by light, scanning, and transmission electron microscopy. Neurites and glial processes were found to be growing both on and between the carbon filaments. The carbon filaments appeared to provide a biocompatible scaffold which promoted adhesion and gave directionality to the growing cell processes. These properties may make carbon filaments a suitable substrate for in vivo implantation into the damaged spinal cord.
Khan
T
T
Neuro-Regeneration Laboratory, Veterans Affairs Hines Hospital, IL 60141.
Sayers
S
S
Gaik
G
G
Dauzvardis
M
M
D000818:Animals; D001672:Biocompatible Materials; D002244:Carbon; D000077482:Carbon Fiber; D005333:Fetus / Q000166:cytology; D066298:In Vitro Techniques; D008853:Microscopy; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D051381:Rats; D013116:Spinal Cord / Q000166:cytology / Q000196:embryology / Q000254:growth & development* / Q000648:ultrastructure
D001672:Biocompatible Materials; D000077482:Carbon Fiber; D002244:Carbon