title: Manipulation of cell cycle kinetics: influence on the cytotoxicity of doxorubicin in human breast cancer cells.
abstract: In vitro exposure of estrogen receptor-negative (ER-) EVSA-T human breast cancer cells to insulin and/or estradiol had no effect on cell cycle distribution, in contrast to a 3-5-fold increase in the percentages of cells in the S-phase of the cell cycle in the ER+ MCF-7 cell line. Estrogen pretreatment of MCF-7 cells followed by incubation with doxorubicin resulted in an augmented inhibition of cell growth compared to unstimulated controls. This delay in growth was accompanied by a decrease in the percentages of cells actively synthesizing DNA, and by an augmented percentage of cells exhibiting a G2M-amount of DNA at the end of a 6-9 day period of culture in complete growth medium.
authors.affiliation: Division of Endocrine Oncology (Department of Medical Oncology), Dr Daniel den Hoed Cancer Center, Rotterdam, The Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: Bontenbal
authors.forename: A M
authors.initials: AM
authors.lastname: Sieuwerts
authors.forename: H A
authors.initials: HA
authors.lastname: Peters
authors.forename: P
authors.initials: P
authors.lastname: Sonneveld
authors.forename: J A
authors.initials: JA
authors.lastname: Foekens
authors.forename: J G
authors.initials: JG
authors.lastname: Klijn
mesh_terms: D001943:Breast Neoplasms / Q000188:drug therapy*; D001973:Bromodeoxyuridine; D002453:Cell Cycle / Q000187:drug effects / Q000235:genetics; D004247:DNA / Q000378:metabolism; D004317:Doxorubicin / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D007700:Kinetics; D011960:Receptors, Estrogen / Q000187:drug effects; D014407:Tumor Cells, Cultured
chemical_list: D007328:Insulin; D011960:Receptors, Estrogen; D004958:Estradiol; D004317:Doxorubicin; D004247:DNA; D001973:Bromodeoxyuridine