Isolation of two new coumarin glycosides from Notopterygium forbesii and evaluation of a Chinese crude drug, qiang-huo, the underground parts of N. incisum and N. forbesii, by high-performance liquid chromatography.
From the ether extract of the underground part of Notopterygium forbesii, two new coumarin glycosides, bergaptol-O-beta-D-glucopyranoside and 6'-O-trans-feruloylnodakenin, were isolated along with known compounds including seven furanocoumarins, two dihydrofuranocoumarins, a sterol glucoside and two phenolic compounds. Analysis of their contents by high-performance liquid chromatography (HPLC) revealed that the underground part of N. forbesii contained large amounts of p-hydroxphenethyl anisate (0.7%), bergaptol glucoside (0.2%), nodakenin (2%) and 6'-O-trans-feruloylnodakenin (0.7%) and a lesser amount of notopterol (0.08%), while that of N. incisum contained a large amount of notopterol (1.2%) and less amounts of the others. The characteristic difference in chemical composition between the two species enabled us to identify the respective botanical sources of a Chinese crude drug, Qiang-huo derived from N. incisum and N. forbesii by HPLC.
Gu
Z M
ZM
School of Pharmacy, Chengdu College of Traditional Chinese Medicine, Sichuan, People's Republic of China.
Zhang
D X
DX
Yang
X W
XW
Hattori
M
M
Namba
T
T
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003374:Coumarins / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D005960:Glucosides / Q000302:isolation & purification*; D006027:Glycosides / Q000302:isolation & purification*; D010936:Plant Extracts / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000032:analysis*
D003374:Coumarins; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D006027:Glycosides; D010936:Plant Extracts; C067415:qiang-huo; C067416:bergaptol-O-glucopyranoside; C067417:6'-feruloylnodakenin