Synthesis and 1H-NMR studies of alpha-deuterated analogues of des-Trp1, Nle12-human minigastrin.
Three analogues of the tridecapeptide amide H-Leu-(Glu)5-Ala-Tyr-Gly-Nle-Asp-Phe-NH2 were synthetized with alpha-deuterated glutamate residues in specific positions in order to assign unambiguously the 1H nmr spectrum of the parent peptide in water and in water-trifluoroethanol mixtures. The synthetic route is described and the assignment illustrated. A previous, tentative assignment based solely on indirect evidence [Mammi, S., Mammi, N. J. & Peggion, E. (1988) Biochemistry 27, 1374-1379] was partially modified.
Amonraksa
K
K
Department of Organic Chemistry, University of Padua, Italy.
Simonetti
M
M
Da Rin-Fioretto
S
S
Mammi
S
S
Peggion
E
E
D000595:Amino Acid Sequence; D003903:Deuterium; D005755:Gastrins / Q000737:chemistry*; D005971:Glutamates / Q000737:chemistry; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D013696:Temperature; D014364:Tryptophan / Q000737:chemistry
D005755:Gastrins; D005971:Glutamates; D010455:Peptides; C008530:minigastrin; D014364:Tryptophan; D003903:Deuterium