Gel electrophoretic analysis of penicillin-binding proteins of coagulase negative staphylococci.
We analyzed gel electrophoretic banding patterns of penicillin-binding proteins (PBPs) of 16 type strains of coagulase-negative staphylococci (CNS). S. epidermidis, S. haemolyticus, S. saprophyticus, S. hominis, S. xylosus, S. simulans, S. warneri, S. capitis, S. saccharolyticus, S. auricularis, S. caseolyticus, S. gallinarum, S. hycus subsp. hycus, S. cohnii, S. caprae, and S. sciuri subsp. sciuri. The PBP profile of each CNS species was found to be unique and was clearly distinguishable from those of the rest of the species. Together with the previous work of other researchers, this study substantiates the applicability of the PBP profile analysis to the identification of clinical CNS strains.
Kanda
K
K
Department of Microbiology, Faculty of Medicine, Juntendo University, Tokyo, Japan.
Hiramatsu
K
K
Yokota
T
T
D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000032:analysis*; D003030:Coagulase / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase / Q000032:analysis*; D046915:Penicillin-Binding Proteins; D010406:Penicillins / Q000378:metabolism*; D010458:Peptidyl Transferases; D013210:Staphylococcus / Q000032:analysis* / Q000145:classification / Q000201:enzymology; D013212:Staphylococcus epidermidis / Q000032:analysis / Q000145:classification / Q000201:enzymology
D001426:Bacterial Proteins; D002352:Carrier Proteins; D003030:Coagulase; D046915:Penicillin-Binding Proteins; D010406:Penicillins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase