Construction of a stable shuttle vector for high-frequency transformation in Pseudomonas syringae pv. syringae.
A cryptic 80.3-kilobase plasmid, pOSU900, in Pseudomonas syringae pv. syringae strain J900 could be cured by treatment with mitomycin without affecting the pathogenicity of J900 on the host, Phaseolus vulgaris L. The replication region of pOSU900 was identified, subcloned, and modified for construction of a high-copy cloning vector. This vector could be transformed into Pseudomonas strains with high efficiency (ca. 10(6) transformants per microgram of DNA) and was very stable during growth of the host bacteria in planta.
Mukhopadhyay
P
P
Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331.
Mukhopadhyay
M
M
Mills
D
D
D003001:Cloning, Molecular; D004261:DNA Replication; D005822:Genetic Vectors; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics*; D014170:Transformation, Genetic