N-linked glycan modification on antigen-presenting cells restores an allospecific cytotoxic T cell response.
The B6 anti-bm6 allospecific CTL response is strictly dependent on CD4+ cells when using LPS blasts as stimulator cells. Altering the N-linked carbohydrates on stimulator cells by use of the N-linked trimming glycosidase inhibitors 1-deoxymannojirimycin and swainsonine, or by treatment with bacterial neuraminidase, results in a restoration of the B6 anti-bm6 response in the absence of CD4+ cells. The extent of restoration is inversely correlated with the number of sialic acids present on N-linked glycans of stimulator cells.
Neefjes
J J
JJ
Division of Cellular Biochemistry, The Netherlands Cancer Institute, Amsterdam.
De Bruijn
M L
ML
Boog
C J
CJ
Nieland
J D
JD
Boes
J
J
Melief
C J
CJ
Ploegh
H L
HL
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D002240:Carbohydrate Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D008285:Major Histocompatibility Complex / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D011134:Polysaccharides