Transcription of human endogenous retroviral long terminal repeat (LTR) sequence in a lung cancer cell line.
The human genome carries several endogenous retroviral sequences. One of them that we named 'HERV-A', carries almost the complete sequence of the long terminal repeat (LTR), and is located in the 5' region of the amylase genes (M.Emi, A.Horii, N.Tomita, T.Nishide, M.Ogawa, T. Mori and K.Matsubara, Gene 62: 229-235, 1988). Using this sequence as a probe, we found a 1.4 kb LTR transcript(s) in a lung cancer cell line. No corresponding transcript was observed in control cells. Two partial, but different cDNA clones were obtained, and each one was found to be a transcript starting within human sequences at 5' upstream from the LTR and ending within the LTR sequence.
Tomita
N
N
Institute for Molecular and Cellular Biology, Osaka University, Suita, Japan.
Horii
A
A
Doi
S
S
Yokouchi
H
H
Ogawa
M
M
Mori
T
T
Matsubara
K
K
D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002460:Cell Line; D003001:Cloning, Molecular; D003106:Colon / Q000032:analysis; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000235:genetics; D015723:Gene Library; D006801:Humans; D008168:Lung / Q000032:analysis; D008175:Lung Neoplasms / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010920:Placenta / Q000032:analysis; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D015342:DNA Probes; D004273:DNA, Neoplasm; D012333:RNA, Messenger