Simple method for determination of the cephalosporin DQ-2556 in biological fluids by high-performance liquid chromatography.
A sensitive method for the determination of DQ-2556 by high-performance liquid chromatography was established. The limits of detection for serum and urine were 0.1 and 2 micrograms/ml, respectively. Two clean-up procedures for serum samples were developed. In the first, deproteinization with 10% trichloroacetic acid was used and the recovery was 68.5%. In the other, ultrafiltration under acidic conditions was employed and the recovery was 85.1%. The former procedure is economical but complicated, whereas the latter is simple and labour-saving, but a special ultrafiltration tube is required. This situation offers a flexible choice, depending on the conditions of the laboratory.
Matsubayashi
K
K
Research Institute, Daiichi Pharmaceutical Co., Ltd., Tokyo, Japan.
Yoshioka
M
M
Tachizawa
H
H
D002511:Cephalosporins / Q000097:blood* / Q000493:pharmacokinetics / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans
D002511:Cephalosporins; C050465:DQ 2556