title: [Solid phase ligation of synthetic DNA fragments].
abstract: A synthetic oligodeoxyribonucleotide (oligo) covalently bound by an internucleotide linkage to the succinylated Sephacryl S-500 support through 1.9-diaminononane spacer was used as starting compound to assemble the E. coli rec A promoter DNA fragment from synthetic oligos by means of T4 DNA ligase. The solid-phase assembly of the designed DNA was performed by two ways: stepwise ligation of two pairs of oligos (2 dyads) or simultaneous ligation of four oligos (tetrad). Both ways gave equal results with some preference in the tetrad case. The reliability of E. coli promoter DNA fragment assembly was demonstrated by cloning it in a plasmid vector and sequencing the cloned DNA by the solid-phase Maxam--Gilbert technique.
authors.forename: S A
authors.initials: SA
authors.lastname: Filippov
authors.forename: S V
authors.initials: SV
authors.lastname: Kalinichenko
authors.forename: D S
authors.initials: DS
authors.lastname: Esipov
authors.forename: E V
authors.initials: EV
authors.lastname: Shibanova
authors.forename: L N
authors.initials: LN
authors.lastname: Shingarova
authors.forename: V G
authors.initials: VG
authors.lastname: Korobko
authors.forename: V N
authors.initials: VN
authors.lastname: Dobrynin
mesh_terms: D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D004247:DNA / Q000138:chemical synthesis* / Q000235:genetics; D011088:DNA Ligases; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013056:Spectrophotometry, Ultraviolet
chemical_list: D009838:Oligodeoxyribonucleotides; D004247:DNA; D011088:DNA Ligases
vernacular_title: Tverdofaznoe ligirovanie sinteticheskikh fragmentov DNK.