title: Different requirements for the regulation of transplantation tolerance induction for allogeneic versus xenogeneic major histocompatibility complex antigens.
abstract: One way to solve the problem of human donor organ shortage is the use of animal organs. Therefore, it is important to study the T-cell response against xenogeneic major histocompatibility complex (MHC) antigens. In the present study, we have used HLA-B27 transgenic mice in a xenogeneic transplantation model. The results indicate that both transgenic skin transplantation and intravenous (IV) injection of transgenic spleen cells can reverse specific T-cell low responsiveness against the transgenic HLA-B27 antigen into high responsiveness in vivo and in vitro. In contrast, IV injection of spleen cells across an allogeneic H-2 class I disparity results in transplantation tolerance. Thus, despite T-cell low responsiveness against the transgenic HLA-B27 antigen, IV injection of transgenic HLA-B27 disparate lymphocytes does not tolerize, but rather immunizes for the xeno-MHC antigen.
authors.affiliation: Central Laboratory, The Netherlands Red Cross Blood Transfusion Service, University of Amsterdam.
authors.forename: E
authors.initials: E
authors.lastname: van Twuyver
authors.forename: R J
authors.initials: RJ
authors.lastname: Mooijaart
authors.forename: W M
authors.initials: WM
authors.lastname: Kast
authors.forename: C J
authors.initials: CJ
authors.lastname: Melief
authors.forename: L P
authors.initials: LP
authors.lastname: de Waal
mesh_terms: D000818:Animals; D001803:Blood Transfusion; D006085:Graft Survival / Q000276:immunology; D015796:HLA-B27 Antigen / Q000235:genetics / Q000276:immunology; D006649:Histocompatibility Antigens / Q000276:immunology*; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007108:Immune Tolerance / Q000276:immunology*; D007114:Immunization; D007694:Killer Cells, Natural / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D016038:Skin Transplantation / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D016378:Tissue Transplantation; D014183:Transplantation, Heterologous / Q000276:immunology*; D014184:Transplantation, Homologous / Q000276:immunology
chemical_list: D015796:HLA-B27 Antigen; D006649:Histocompatibility Antigens