Hemagglutination activities of purified pertussis toxin and filamentous hemagglutinin against erythrocytes from various animals.
The hemagglutinating (HA) activities of purified pertussis toxin (PT) and filamentous hemagglutinin (FHA) were evaluated against unfixed and glutaraldehyde-fixed erythrocytes from ox, goose, horse, monkey, sheep, chicken, and rabbit. Both PT and FHA showed HA activities against fixed and unfixed erythrocytes from all the animals studied. The HA titers of FHA were higher than those of PT. The HA activities of FHA and PT were not destroyed completely even after heating these preparations at 56 C for 30 min. A simple test for the assay of PT in culture supernatants of Bordetella pertussis on the basis of HA activity has been described.
Gupta
R K
RK
Biological Standardization and Quality Control Division, Central Research Institute, Kasauli, India.
Saxena
S N
SN
Sharma
S B
SB
Ahuja
S
S
D000818:Animals; D004912:Erythrocytes / Q000187:drug effects*; D006384:Hemagglutination / Q000187:drug effects*; D006386:Hemagglutination Tests; D006388:Hemagglutinins / Q000494:pharmacology*; D006358:Hot Temperature; D037342:Pertussis Toxin; D011489:Protein Denaturation; D010566:Virulence Factors, Bordetella / Q000032:analysis / Q000494:pharmacology*
D006388:Hemagglutinins; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin