[Monoclonal antibody against laminin a and b chains: preparation and its clinical application].
In present study, we developed a hybridoma cell line which secretes monoclonal antibody against Laminin (Mo-611). The MoAb produced was identified to be isotype of IgG1. It reacted with Laminin obtained from murine EHS sarcoma, the intensity of reaction declined in accompany with increment of dilution, and could be absorbed by Laminin in 1 u/ml. On western blotting, it bounded to Laminin A chain 400 KD and Laminin B chain 200 KD. On IF study of renal tissue, positive staining with Mo-611 could be detected in fetal GBM and TBM, normal adult glomerular mesangial area and cytoplasm of tubular epithelium. In case of IgA nephropathy, the amount of deposits in mesangial area increased while in membranous nephropathy deposits along the capillary loop was also abundant in amount. In conclusion, the clinical application of McAb against Laminin might be helpful in the immunopathological diagnosis of glomerular diseases.
Chen
X M
XM
Department of Nephrology, Chinese PLA General Hospital, Beijing.
Yu
L F
LF
Tian
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D005922:Glomerulonephritis, IGA / Q000175:diagnosis; D015433:Glomerulonephritis, Membranous / Q000175:diagnosis; D007797:Laminin / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D007797:Laminin