title: Rat liver nuclei protein kinase C is the isozyme type II.
abstract: Rat liver nuclei protein kinase C is identified as type II isozyme employing monospecific antibodies obtained against each three types of rat brain protein kinase C isozymes. (Yoshida, Y., Huang, F. L., Nakabayashi, H., and Huang, K-P. (1988) J. Biol. Chem. 263, 9868-9873). A major immunoreactive protein band at 80 kDa was revealed by type II isozyme antibodies at each step of purification, nuclear extract included. The nuclear protein kinase C has been purified to apparent homogeneity as revealed by silver nitrate staining on sodium dodecyl sulfate-polyacrylamide gel electrophoresis showing a single 80 kDa protein band. It does seem that 66 kDa protein (Masmoudi, A., Labourdette, G., Mersel, M., Huang, F. L., Huang, K.-P., Vincendon, G., and Malviya, A. N. (1989) J. Biol. Chem. 264, 1172-1179) is a major contaminant devoid of any protein kinase activity. The ratio obtained between protein kinase C enzymatic activity over phorbol dibutyrate bound, at various purification steps, indicates that the nuclear enzyme is a phorbol ester receptor. When isolated nuclei were incubated with 12-O-tetradecanoyl phorbol-13-acetate, endogenous protein kinase C activity was elevated about 8-10-fold suggesting the existence of phorbol ester signaling pathway at the level of nucleus. The role of nuclear protein kinase C is delineated in the regulation of inducible gene transcription
authors.affiliation: Centre de Neurochimie du Centre National de la Recherche Scientifique, Unité 44 de l'Institut National de la Santé et de la Recherche Médicale, Strasbourg, France.
authors.forename: P
authors.initials: P
authors.lastname: Rogue
authors.forename: G
authors.initials: G
authors.lastname: Labourdette
authors.forename: A
authors.initials: A
authors.lastname: Masmoudi
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshida
authors.forename: F L
authors.initials: FL
authors.lastname: Huang
authors.forename: K P
authors.initials: KP
authors.lastname: Huang
authors.forename: J
authors.initials: J
authors.lastname: Zwiller
authors.forename: G
authors.initials: G
authors.lastname: Vincendon
authors.forename: A N
authors.initials: AN
authors.lastname: Malviya
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology*; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D007527:Isoenzymes / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D011493:Protein Kinase C / Q000302:isolation & purification* / Q000378:metabolism; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D007527:Isoenzymes; D015240:Phorbol 12,13-Dibutyrate; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate