title: Cellular oncogenes in human teratocarcinoma cell lines.
abstract: We have analysed, by Northern blots, the expression of 14 cellular oncogenes in nine cell lines established from human teratocarcinomas. All lines expressed considerable amounts of p53, c-Ki-ras2, c-Ha-ras1, c-raf1, N-myc, and c-fos. Low level expression of c-myc was detected in some lines. Southern blot experiments revealed no amplification or rearrangement of the c-Ki-ras2, N-myc or c-fos genes. Using a rapid dot-blot screening procedure, based on a combination of in-vitro amplification of ras-specific sequences and oligonucleotide hybridization, we could detect no activation of Ha-ras or Ki-ras or any unexpressed N-ras sequences secondary to a point mutation at codons 12, 13, or 61.
authors.affiliation: Medizinische Klinik, Universität Köln, FRG.
authors.forename: H
authors.initials: H
authors.lastname: Tesch
authors.forename: R
authors.initials: R
authors.lastname: Fürbass
authors.forename: J
authors.initials: J
authors.lastname: Casper
authors.forename: J
authors.initials: J
authors.lastname: Lyons
authors.forename: C R
authors.initials: CR
authors.lastname: Bartram
authors.forename: H J
authors.initials: HJ
authors.lastname: Schmoll
authors.forename: D L
authors.initials: DL
authors.lastname: Bronson
mesh_terms: D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000302:isolation & purification; D015870:Gene Expression; D011905:Genes, ras / Q000235:genetics; D006801:Humans; D008297:Male; D009154:Mutation; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D009857:Oncogenes / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012334:RNA, Neoplasm / Q000302:isolation & purification; D013724:Teratoma / Q000235:genetics*; D013736:Testicular Neoplasms / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D015342:DNA Probes; D004273:DNA, Neoplasm; D015345:Oligonucleotide Probes; D012334:RNA, Neoplasm