Removing oxygen-derived free radicals stimulates healing of ethanol-induced erosive gastritis in the rat.
The effect of the radical removing agents, allopurinol and dimethyl sulphoxide (DMSO), on the healing rate of ethanol (1 ml of 40% solution) induced gastric mucosal injury was studied in the rat. One millilitre of 1, 2 or 5% allopurinol or DMSO were instilled into the stomach 1, 24 and 48 h after giving ethanol by gavage. One hour after administration of ethanol, gastric mucosal injury was produced in all animals (20.4 +/- 1.2 mm2, mean +/- SEM, n = 10). Treatment for 24 h with 2% allopurinol or DMSO significantly (p less than 0.01) reduced the extent of the ethanol injury (11.1 +/- 0.8 and 11.9 +/- 0.9 mm2, respectively, vs. 19.2 +/- 1.1 mm2, n = 10) and this was similarly achieved by the 5% solutions (10.4 +/- 0.9 and 10.2 +/- 0.8 mm2, respectively, vs. 19.2 +/- 1.1 mm2, n = 10). After treatment for 48 h, 30% of animals having 1% allopurinol or DMSO remained with injury significantly (p less than 0.001) less than that seen with ethanol alone (4.1 +/- 0.4 and 3.9 +/- 0.5 mm2, respectively, vs. 12.1 +/- 0.9 mm2, n = 10); however, none treated with 2 or 5% solutions remained with any injury. Healing of this injury was confirmed microscopically and was achieved by regeneration. Thus, removing oxygen-derived free radicals stimulates the healing of ethanol-induced acute gastric mucosal injury in the rat.
Salim
A S
AS
University Department of Surgery, Royal Infirmary, Glasgow, UK.
D000493:Allopurinol / Q000627:therapeutic use*; D000818:Animals; D004121:Dimethyl Sulfoxide / Q000627:therapeutic use*; D000431:Ethanol / Q000009:adverse effects*; D005260:Female; D016166:Free Radical Scavengers; D005609:Free Radicals; D005756:Gastritis / Q000188:drug therapy* / Q000209:etiology; D008297:Male; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014945:Wound Healing / Q000187:drug effects*
D016166:Free Radical Scavengers; D005609:Free Radicals; D000431:Ethanol; D000493:Allopurinol; D010100:Oxygen; D004121:Dimethyl Sulfoxide