Elevated expression of AP-1 activity in human breast tumors as compared to normal adjacent tissue.
The levels of AP-1 activity in human breast lesions and in adjacent normal tissue were studied by a gel retardation assay. A thirty nucleotide long consensus oligonucleotide to the adenovirus E3 gene AP-1 sequence (E3AP-1) was end labelled and reacted with nuclear extracts from breast lesions and adjacent normal tissue. A total of 20 tissue extracts (8 pairs of tumor and normal tissue from the same patient and 4 tumors) were examined. All 12 tumor tissues showed elevated levels of AP-1 as compared to the 8 normal tissues. These results suggest that the AP-1 transcription factor may play a role in breast neoplasia.
Linardopoulos
S
S
Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, Athens, Greece.
Malliri
A
A
Pintzas
A
A
Vassilaros
S
S
Tsikkinis
A
A
Spandidos
D A
DA
D000260:Adenoviruses, Human / Q000235:genetics; D001483:Base Sequence; D001940:Breast / Q000378:metabolism / Q000473:pathology; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D009367:Neoplasm Staging; D015345:Oligonucleotide Probes; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics*; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*; D012016:Reference Values
D015345:Oligonucleotide Probes; D011506:Proteins; D016755:Proto-Oncogene Proteins c-jun