Regucalcin-induced Ca2+ release from rat liver microsomes: the effect is inhibited by heparin.
The effect of heparin on the calcium-binding protein regucalcin-stimulated Ca2+ release from rat liver microsomes was investigated. Ca2+ release was assayed by the method of Millipore filtration to estimate microsomal 45Ca2+ accumulation following the addition of 10 mM adenosine triphosphate. The addition of regucalcin (1.0 microM) or inositol 1,4,5-trisphosphate [Ins(1,4,5)P3; 1.0 microM] stimulated 45Ca2+ release from rat liver microsomes. These effects were completely inhibited by the presence of heparin (10.0 micrograms/ml). Regucalcin did not enhance the effect of Ins(1,4,5)P3. These results suggest that regucalcin affects 45Ca2+ release involved in Ins(1,4,5)P3 action in rat liver microsomes.
Yamaguchi
M
M
Department of Environmental Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
Mori
S
S
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000494:pharmacology*; D002265:Carboxylic Ester Hydrolases; D004347:Drug Interactions; D006493:Heparin / Q000494:pharmacology*; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D015238:Sulfotransferases
D002135:Calcium-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; D006493:Heparin; D015238:Sulfotransferases; C028152:alcohol sulfotransferase; D002265:Carboxylic Ester Hydrolases; C513325:Rgn protein, rat; D002118:Calcium