Ascorbic acid modulates the production of fibronectin and laminin by cells from an eye tissue-trabecular meshwork.
Ascorbic acid is a significant component in the aqueous humor of the eye, in which trabecular meshwork cells are immersed. Using immunostaining and Western blot analysis, the effects of ascorbic acid on the extracellular matrix production by cultured bovine trabecular meshwork cells were examined. It was found that, when the cultures were treated with 100, 250, and 500 micrograms/ml of ascorbic acid, the amount of fibronectin and laminin produced was enhanced. An enzyme-linked immunosorbent assay for quantitation further showed that the fibronectin and laminin levels in ascorbate-treated cultures were higher than those found in control cultures. These results indicate that ascorbic acid promotes fibronectin and laminin production and suggest that this substance may be one of the factors modulating basal lamina assembly in the trabecular meshwork.
Yue
B Y
BY
University of Illinois, College of Medicine, Department of Ophthalmology, Eye Research Institute, Chicago.
Higginbotham
E J
EJ
Chang
I L
IL
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D015153:Blotting, Western; D002417:Cattle; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005353:Fibronectins / Q000096:biosynthesis* / Q000302:isolation & purification; D007797:Laminin / Q000096:biosynthesis* / Q000302:isolation & purification; D014129:Trabecular Meshwork / Q000187:drug effects / Q000378:metabolism*
D005353:Fibronectins; D007797:Laminin; D001205:Ascorbic Acid