title: Interaction of extracellular vesicles of Bacteroides gingivalis W50 with human polymorphonuclear leucocytes.
abstract: The effects of B. gingivalis W50 extracellular vesicles (ECV) on neutrophil chemotaxis and viability were assessed and compared with those of whole cells and the extracellular non-dialysable soluble protein (EP) fraction. None of the fractions tested, including soluble fractions derived from cells and ECV by sonication, induced neutrophil chemotaxis. Only ECV and cells inhibited f-MLP-stimulated chemotaxis. ECV and cells were cytotoxic towards neutrophils. The cytotoxic response was time dependent. The soluble EP fraction did not influence cell viability.
authors.affiliation: Department of Clinical Dental Sciences, University of Liverpool, U.K.
authors.forename: H M
authors.initials: HM
authors.lastname: Kay
authors.forename: A J
authors.initials: AJ
authors.lastname: Birss
authors.forename: J W
authors.initials: JW
authors.lastname: Smalley
mesh_terms: D001439:Bacteroides / Q000472:pathogenicity* / Q000648:ultrastructure; D002470:Cell Survival; D002634:Chemotaxis, Leukocyte; D006801:Humans; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009504:Neutrophils / Q000166:cytology / Q000502:physiology*; D012995:Solubility; D014774:Virulence
chemical_list: D009240:N-Formylmethionine Leucyl-Phenylalanine