An intracellular pathway is required for ABA-tyrosine presentation to T cells.
Although tyrosine-azobenzenearsonate (ABA-Tyr) is not degraded by proteolytic enzymes, its presentation by accessory cells is inhibited by lysosomotropic agents such as chloroquine. Presentation of ABA-poly-L-glutamic, alanine, tyrosine (ABA-GAT) is similarly inhibited by chloroquine, but in contrast to ABA-Tyr it is also inhibited by leupeptin. Finally formaldehyde fixation of accessory cells after pulsing with ABA-Tyr but not before permits successful stimulation of ABA-specific hybridoma cells. These results suggest that a lysosomal pathway but not digestion is necessary for the association of ABA-Tyr and la molecules for presentation.
Joshi
N
N
Department of Pathology, Tufts University School of Medicine, Boston, Massachusetts 02111.
Baskar
S
S
Leskowitz
S
S
D000818:Animals; D000938:Antigen-Presenting Cells / Q000378:metabolism*; D001391:Azo Compounds / Q000378:metabolism*; D002460:Cell Line; D002738:Chloroquine / Q000494:pharmacology; D005557:Formaldehyde / Q000494:pharmacology; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism; D010132:p-Azobenzenearsonate / Q000031:analogs & derivatives / Q000378:metabolism*
D001391:Azo Compounds; D000949:Histocompatibility Antigens Class II; D005557:Formaldehyde; C000229:tyrosine-4-azobenzenearsonate; D014443:Tyrosine; D010132:p-Azobenzenearsonate; D002738:Chloroquine