title: Initiation of growth in pbpAts and rodAts mutants of Escherichia coli.
abstract: Escherichia coli strains harbouring pbpAts mutations are particularly sensitive to functional alterations of penicillin-binding protein 2 (PBP 2) at the initiation of growth. Shift-up to 42 degrees C results in the inability of cells to reach a steady rate of growth and division. Furthermore, a very high proportion of cells generate minicell-like structures which are pinched-off through a process requiring the activity of penicillin-binding protein 3 (PBP 3).
authors.affiliation: Centro de Biología Molecular CSIC-UAM, Facultad de Ciencias, Madrid, Spain.
authors.forename: M C
authors.initials: MC
authors.lastname: Rodriguez
authors.forename: M A
authors.initials: MA
authors.lastname: de Pedro
mesh_terms: D001390:Azlocillin / Q000031:analogs & derivatives / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002352:Carrier Proteins; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D029968:Escherichia coli Proteins; D006602:Hexosyltransferases / Q000235:genetics* / Q000378:metabolism; D048289:Imidazolidines; D007700:Kinetics; D008565:Membrane Proteins; D009097:Multienzyme Complexes / Q000235:genetics* / Q000378:metabolism; D002267:Muramoylpentapeptide Carboxypeptidase; D009154:Mutation; D046915:Penicillin-Binding Proteins; D010406:Penicillins / Q000378:metabolism*; D046928:Peptidoglycan Glycosyltransferase; D010458:Peptidyl Transferases / Q000235:genetics* / Q000378:metabolism; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; C478704:FtsI protein, E coli; D048289:Imidazolidines; D008565:Membrane Proteins; D009097:Multienzyme Complexes; D046915:Penicillin-Binding Proteins; D010406:Penicillins; C061683:mrdB protein, E coli; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D046928:Peptidoglycan Glycosyltransferase; D002267:Muramoylpentapeptide Carboxypeptidase; D001390:Azlocillin; C017976:furazlocillin