title: Isoenzyme pattern of lactate dehydrogenase associated with human prostasomes.
abstract: Lactate dehydrogenase (LD, EC 1.1.1.27) activity was determined in serum, seminal plasma and prostasomes from normozoospermic and azoospermic (due to vasectomy) men. A 10-fold higher activity was noted in seminal plasma as compared with serum. LD activity was enriched in prostasomes although a fairly large amount of enzyme activity seemed to be free in solution. Isoenzymes LD1, LD2 and LD3, present in prostasomes in about equal amounts, gave rise to a characteristic isoenzyme pattern that differed distinctly from that found in seminal plasma of normozoospermic men, the main isoenzyme of which was LD-X.
authors.affiliation: Pharmacia-LKB AB, Uppsala, Sweden.
authors.forename: I
authors.initials: I
authors.lastname: Olsson
authors.forename: G
authors.initials: G
authors.lastname: Ronquist
mesh_terms: D001826:Body Fluids / Q000201:enzymology; D002850:Chromatography, Gel; D004586:Electrophoresis; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008297:Male; D009845:Oligospermia / Q000201:enzymology; D015388:Organelles / Q000201:enzymology; D011467:Prostate / Q000201:enzymology*; D012661:Semen / Q000201:enzymology*
chemical_list: D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase