Intraluminal release of PGE2 due to gastric perfusion of urethane-anaesthetized rats with hydrochloric or taurocholic acid.
We have measured the intragastric release of prostaglandin E2-like immunoreactivity (PGE2-li), pH changes and mucosal damage in response to gastric perfusion with hydrochloric or taurocholic acid at pH 1.5 in urethane-anaesthetized rats. Hydrochloric acid-perfusion caused a reversible decrease in pH values, no damage to the gastric mucosa and an intraluminal release of PGE2-li. Exposure to an ulcerogenic dose (25 mM) of taurocholic acid produced an intraluminal release of PGE2-li much higher than that of the non-ulcerogenic perfusion with hydrochloric acid. These findings indicate that intraluminal release of PGE2-li, thought to play a role as an endogenous antiulcer factor, can occur in response to a stimulus producing a significant damage of the gastric mucosa or to a mild irritant stimulus.
Evangelista
S
S
Pharmacology Dept., Menarini Pharmaceuticals, Florence, Italy.
Renzi
D
D
Lippe
I T
IT
Mantellini
P
P
Guzzi
P
P
Maggi
C A
CA
D000758:Anesthesia; D000818:Animals; D015232:Dinoprostone / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism*; D006851:Hydrochloric Acid / Q000008:administration & dosage* / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007124:Immunoenzyme Techniques; D008297:Male; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D013276:Stomach Ulcer / Q000139:chemically induced; D013656:Taurocholic Acid / Q000008:administration & dosage* / Q000494:pharmacology; D013997:Time Factors; D014520:Urethane
D014520:Urethane; D013656:Taurocholic Acid; D015232:Dinoprostone; D006851:Hydrochloric Acid