Detection of estrogen receptors in the trigonum and urinary bladder with an immunohistochemical technique.
In order to detect estrogen receptors (ER) in the female bladder, 5 premenopausal and 10 postmenopausal women affected by gynecological diseases were submitted to cystoscopy, during which both the trigonum and the bladder lateral wall were biopsied. A new, simple, cost-effective immunohistochemical technique was employed to stain the estrogen specific binding sites. ER were found in the trigonum of 3 premenopausal and 4 postmenopausal subjects, while the bladder lateral wall was always ER-negative. A comparison with previously used ER detection methods and a discussion of further hypothetical applications of the immunohistochemical technique in the study of the lower female urogenital tract are reported.
Bussolati
G
G
Department of Biomedical Sciences and Human Oncology, University of Turin, Ospedale Mauriziano Umberto I, Italy.
Tizzani
A
A
Casetta
G
G
Cassoni
P
P
Pacchioni
D
D
Piana
P
P
Revelli
A
A
Massobrio
M
M
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D004967:Estrogens / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D011960:Receptors, Estrogen / Q000378:metabolism*; D001743:Urinary Bladder / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D004967:Estrogens; D011960:Receptors, Estrogen