Transforming growth factor-beta 1 and T-lymphocytes modulate the growth of human peripheral blood progenitor cells.
This study examined the effects of transforming growth factor-beta 1 (TGF-beta 1) and autologous T-lymphocytes on the growth of day-14 granulocyte-monocyte progenitor cells (CFU-GM). Mononuclear cells from human peripheral blood were depleted of accessory cells, yielding "B/null" or "null" cell populations enriched 17-fold and 94-fold, respectively, for CFU-GM. Addition of TGF-beta 1 to B/null or null cells caused a dose-dependent decline in CFU-GM cloning efficiency. The addition of unstimulated autologous T cells to B/null or null cells enhanced CFU-GM growth both in the absence and presence of TGF-beta 1. T cells enhanced growth of all CFU-GM colony types to equal extents, whereas TGF-beta 1 inhibition preferentially reduced the proportion of macrophage colonies. TGF-beta 1 concentrations that blocked T-cell proliferation did not interfere with the capacity of T cells to enhance CFU-GM growth. The data suggest that noncycling T cells can reduce the inhibitory effects of TGF-beta 1, most likely through production of one or more stimulatory cytokines.
Dooley
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DC
Transplantation Laboratory, American Red Cross, Jerome Holland Laboratory for Biomedical Sciences, Rockville, Maryland 20855.
Law
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P
D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D006098:Granulocytes / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D009000:Monocytes / Q000166:cytology*; D013601:T-Lymphocytes / Q000166:cytology / Q000502:physiology*; D015291:Transforming Growth Factors / Q000494:pharmacology*
D003470:Culture Media; D015291:Transforming Growth Factors