Crystallization and preliminary X-ray diffraction studies of C-phycocyanin from a red alga, Porphyra tenera.
C-Phycocyanin from a red alga, Porphyra tenera, has been crystallized by the vapor-diffusion procedure. Both orthorhombic and hexagonal forms were obtained from ammonium sulfate solutions, whereas only the orthohombic form was selectively grown from sodium citrate solutions. The orthorhombic crystals are more suitable for further crystallographic work; their space group is P2(1)2(1)2(1), with unit-cell dimensions of a = 105, b = 121, and c = 184 A. The asymmetric unit comprises two (alpha beta)3 trimer molecules of C-phycocyanin. These crystals diffract X-rays up to about 3 A resolution.
Miki
K
K
Department of Applied Chemistry, Faculty of Engineering, Osaka University.
Ezoe
T
T
Masui
A
A
Yoshisaka
T
T
Mimuro
M
M
Fujiwara-Arasaki
T
T
Kasai
N
N
D004058:Diffusion; D010798:Phycocyanin / Q000737:chemistry*; D000461:Rhodophyta / Q000032:analysis*; D014835:Volatilization; D014961:X-Ray Diffraction
D010798:Phycocyanin