Increased frequency of 6-thioguanine-resistant peripheral blood lymphocytes in Werner syndrome patients.
The frequency of spontaneous 6-thioguanine (TG)-resistant peripheral blood lymphocytes in five unrelated Werner syndrome (WS) patients was determined using an autoradiographic labeling assay. The average frequency of TG-resistant lymphocytes was eightfold higher in WS patients than in sex- and age-matched normal control donors. This finding and previous identification of increased spontaneous chromosomal rearrangements and deletions in WS cells or cell lines suggest that WS is a human genomic instability or mutator syndrome.
Fukuchi
K
K
Department of Medicine and Geriatrics, Osaka University Medical School, Japan.
Tanaka
K
K
Kumahara
Y
Y
Marumo
K
K
Pride
M B
MB
Martin
G M
GM
Monnat
R J
RJ
D000328:Adult; D002478:Cells, Cultured; D004351:Drug Resistance; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D013866:Thioguanine / Q000633:toxicity*; D014898:Werner Syndrome / Q000235:genetics*
D013866:Thioguanine