Lack of specificity of DA/DAPI fluorescence.
Cytogenetic studies showed 47,XY, + mar in a developmentally retarded child with some features of Prader-Willi syndrome, and 46,XX in his mother. The marker chromosome showed a single subterminal primary constriction, bisatellites, and two C-bands. DA/DAPI staining showed two intense bands in the marker chromosome, which most likely was derived from chromosome 15. Intense DA/DAPI fluorescence was also found in one chromosome 13 in the child, and one 13 and one 10 in his mother. The present results confirm the reports of DA/DAPI heteromorphism in acrocentric chromosomes other than the 15, and demonstrate a pericentric DA/DAPI heteromorphism in chromosome 10.
Lin
M S
MS
Department of Pediatrics, LAC/USC Medical Center.
Huynh
K H
KH
Fujimoto
A
A
Wilson
M G
MG
D000328:Adult; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002879:Chromosomes, Human, Pair 10; D002882:Chromosomes, Human, Pair 13; D002884:Chromosomes, Human, Pair 15; D004214:Distamycins; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D007211:Indoles; D007223:Infant; D008607:Intellectual Disability / Q000235:genetics*; D007621:Karyotyping; D008297:Male; D011237:Predictive Value of Tests; D011758:Pyrroles
D004214:Distamycins; D005456:Fluorescent Dyes; D007211:Indoles; D011758:Pyrroles; C007293:DAPI; C030000:stallimycin