Genomic localization of hog cholera virus glycoproteins.
A polyspecific antiserum has been used to identify four different glycoproteins in hog cholera virus (HCV)-infected cells termed gp55, gp48, gp44, and gp33 (Rümenapf et al, 1989, Virology 171, 18-27). Fusion proteins containing parts of the putative HCV-encoded glycoproteins were expressed in bacteria and served for the preparation of specific antibodies. These were used in radioimmunoprecipitation assays which revealed that gp48 and gp44 most likely share a common protein backbone. The order of the glycoproteins on the HCV genome was determined as follows: NH2-gp44/gp48-gp33-gp55-COOH.
Stark
R
R
Federal Research Centre for Virus Diseases of Animals, Tübingen, Federal Republic of Germany.
Rümenapf
T
T
Meyers
G
G
Thiel
H J
HJ
D000818:Animals; D015153:Blotting, Western; D006692:Classical Swine Fever Virus / Q000235:genetics*; D005814:Genes, Viral; D006023:Glycoproteins / Q000235:genetics*; D011233:Precipitin Tests; D015531:Radioimmunoprecipitation Assay; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics*
D006023:Glycoproteins; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins