Skeletal keratan sulfate chain molecular weight calibration by high-performance gel-permeation chromatography.
A method has been developed for the molecular sizing of skeletal keratan sulfate chains using an HPLC gel-permeation chromatography system. Keratan sulfate chains and keratanase-derived oligosaccharides were prepared from the nucleus pulposus of bovine intervertebral disc (6-year-old animals). A Bio-Gel TSK 30 XL column eluted in 0.2 M NaCl and at 30 degrees C was calibrated with keratan sulfate oligosaccharides of known size as well as 3H-end-labeled keratan sulfate chains to yield the relationship.
Dickenson
J M
JM
Division of Biological Sciences, Lancaster University, Bailrigg, United Kingdom.
Morris
H G
HG
Nieduszynski
I A
IA
Huckerby
T N
TN
D000818:Animals; D002138:Calibration; D002417:Cattle; D002467:Cell Nucleus / Q000737:chemistry*; D011508:Chondroitin Sulfate Proteoglycans / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D007632:Keratan Sulfate / Q000737:chemistry*; D000071234:Lumican; D008970:Molecular Weight; D009844:Oligosaccharides / Q000737:chemistry; D013131:Spine / Q000737:chemistry; D014316:Tritium
D011508:Chondroitin Sulfate Proteoglycans; D000071234:Lumican; D009844:Oligosaccharides; D014316:Tritium; D007632:Keratan Sulfate