[Somatotropic cell differentiation in an organ culture of the chick embryo adenohypophysis].
Morphogenetic potencies of the adenohypophysis tissue from 4.5 to 11-day old chicken embryos used for the differentiation of somatotropic cells were investigated by methods of organ tissue culture. STG-cells were detected in cultures by immunofluorescence using an antiserum to human STG. In vitro studies of organ cultures revealed differentiation of STG-cells when adenohypophysis tissue was cultured from the 5.5th, 7th, 9th and 11th day of development in the absence of the diencephalon. Differentiation of STG-cells occurred predominantly in embryo caudal lobe transplants after chorion-allantois culturing of Rathke's pocket fragments from 4.5-, 5.0- and 5.5-day old embryos. The data obtained suggest that at late stages of Rathke's pocket development differentiation of STG-cells is preprogrammed and that determined precursors of these cells are located in the caudal lobe of the germ.
Barabanov
V M
VM
D000482:Allantois; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002642:Chick Embryo; D002823:Chorion; D003470:Culture Media; D005455:Fluorescent Antibody Technique; D013006:Growth Hormone / Q000378:metabolism*; D009024:Morphogenesis / Q000502:physiology; D009924:Organ Culture Techniques / Q000379:methods; D010903:Pituitary Gland, Anterior / Q000166:cytology* / Q000378:metabolism; D013997:Time Factors
D003470:Culture Media; D013006:Growth Hormone
Differentsirovka somatotropnykh kletok v organnoĭ kul'ture adenogipofiza kurinykh zarodysheĭ.