title: Tumor necrosis factor and wound healing.
abstract: Tumor necrosis factor alpha (TNF), 1 to 500 ng in saline (PBS) or collagen, was applied to the wounds of normal and Adriamycin-impaired mice and wound disruption strength (WDS) and histology were examined. Also wounded mice were administered TNF 25 to 75 micrograms/kg IP daily and WDS was determined. Wound histology was examined 6 months after wounding and local TNF application. Local TNF 5 to 500 ng in PBS did not significantly affect WDS. Local TNF 5 to 50 ng in collagen increased WDS 33% to 65% in Adriamycin-impaired animals (p = 0.05 to p less than 0.02). Local TNF 50 to 500 ng in collagen increased WDS 23% to 49% in normal animals (p = 0.08 to p less than 0.01). Adriamycin-impaired animals demonstrated improved wound histology with local TNF in collagen. Systemic TNF did not significantly affect WDS. Local TNF in collagen did not induce histologic pathology at 6 months. TNF may modulate macrophage function and local TNF in collagen can improve WDS in normal and Adriamycin-impaired animals.
authors.affiliation: Department of Surgery, University of Vermont, Burlington 05401.
authors.forename: D P
authors.initials: DP
authors.lastname: Mooney
authors.forename: M
authors.initials: M
authors.lastname: O'Reilly
authors.forename: R L
authors.initials: RL
authors.lastname: Gamelli
mesh_terms: D000287:Administration, Topical; D000818:Animals; D003094:Collagen / Q000008:administration & dosage; D004317:Doxorubicin / Q000494:pharmacology; D006097:Granulation Tissue / Q000166:cytology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011897:Random Allocation; D012867:Skin / Q000293:injuries; D012965:Sodium Chloride / Q000008:administration & dosage; D014409:Tumor Necrosis Factor-alpha / Q000008:administration & dosage / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D012965:Sodium Chloride; D004317:Doxorubicin; D003094:Collagen