Clinical concentrations of verapamil affect the in vitro diagnosis of susceptibility to malignant hyperpyrexia.
We examined the effects of verapamil on the in vitro caffeine and halothane tests for malignant hyperpyrexia (MH) susceptibility. Ten consecutive MH-susceptible patients were investigated according to the protocol of the European MH group. Additional tests were carried out in the presence of verapamil 10(-6) mol litre-1. In four of the 10 patients, the halothane contracture response following pretreatment with verapamil was classified as positive to halothane. In contrast, in nine of the 10 patients, contracture tests of muscle in the presence of verapamil were classified as negative to caffeine. It is advised that verapamil should be discontinued before performing a contracture test.
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P J
PJ
Département d'Anesthésie Réanimation Chirurgicale, Centre Hospitalier Universitaire, Lille, France.
Krivosic-Horber
R M
RM
Adamantidis
M M
MM
Haudecoeur
G
G
Reyfort
G H
GH
Dupuis
B A
BA
D002110:Caffeine; D004198:Disease Susceptibility / Q000175:diagnosis; D005188:False Negative Reactions; D006221:Halothane; D006801:Humans; D066298:In Vitro Techniques; D008305:Malignant Hyperthermia / Q000175:diagnosis*; D009119:Muscle Contraction / Q000187:drug effects; D012684:Sensory Thresholds / Q000187:drug effects; D014700:Verapamil / Q000494:pharmacology*
D002110:Caffeine; D014700:Verapamil; D006221:Halothane