S100 protein expression in human melanoma cells: comparison of levels of expression among different cell lines and individual cells in different phases of the cell cycle.
The synthesis of S100 protein in cultured human melanoma cells was examined using metabolic labeling with [35S]methionine, immunoprecipitation with anti-S100 protein antiserum, and polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Six of seven cell lines derived from melanomas synthesized relatively large amounts of S100 protein, whereas three cell lines derived from normal melanocytes synthesized lesser amounts. Synthesis of S100 protein was not detected in 10 human cell lines of nonneuroectodermal origin. Analysis of poly(A+) RNA from one melanoma cell line by Northern blot hybridization with a probe specific for the beta subunit of rat S100 protein revealed a single mRNA species of 1.0 kb coding for the human protein. Flow cytometric analysis of individual cells of two melanoma cell lines and the rat glioma cell line C6 indicated that G0/G1 cells were heterogeneous with respect to S100 protein expression, while almost all the cells in S + G2 + M expressed S100 protein. These results suggest that expression of S100 protein in G0/G1 could be a prerequisite for progression of the cells through the cell cycle.
Marks
A
A
Banting and Best Department of Medical Research, University of Toronto, Canada.
Petsche
D
D
O'Hanlon
D
D
Kwong
P C
PC
Stead
R
R
Dunn
R
R
Baumal
R
R
Liao
S K
SK
D015152:Blotting, Northern; D002453:Cell Cycle; D002460:Cell Line; D005434:Flow Cytometry; D006801:Humans; D008544:Melanocytes / Q000378:metabolism; D008545:Melanoma; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D009418:S100 Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000378:metabolism*
D009418:S100 Proteins