Protein kinase C activity in the gerbil hippocampus after transient forebrain ischemia: morphological and autoradiographic analysis using [3H]phorbol 12,13-dibutyrate.
The influence of transient forebrain ischemia on the temporal alteration of protein kinase C (PKC) activity in the gerbil hippocampus was analyzed by quantitative autoradiography using [3H]phorbol 12,13-dibutyrate (PDBu). The [3H]PDBu binding activity in the stratum oriens of the CA1 subfield increased at 6 h after ischemia, but the binding activity in this subfield decreased at 7 days after ischemia. In contrast, the [3H]PDBu binding activity increased in the molecular layer of the dentate gyrus at 7 days after ischemia. Pre-treatment of pentobarbital prevented an increase in the [3H]PDBu binding activity in the stratum oriens of the CA1 subfield at 7 days after ischemia. These results indicate the possibilities that PKC may play a pivotal role in the post-ischemic neuronal damage in the hippocampal CA1 subfield.
Hara
H
H
Department of Neurology, Tohoku University School of Medicine, Sendai, Japan.
Onodera
H
H
Kogure
K
K
D000818:Animals; D001345:Autoradiography / Q000379:methods; D001665:Binding Sites; D005849:Gerbillinae; D006624:Hippocampus / Q000201:enzymology* / Q000473:pathology; D002546:Ischemic Attack, Transient / Q000201:enzymology* / Q000473:pathology; D009928:Organ Specificity; D015240:Phorbol 12,13-Dibutyrate / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D011712:Pyramidal Tracts / Q000201:enzymology / Q000473:pathology; D015424:Reperfusion; D014316:Tritium
D014316:Tritium; D015240:Phorbol 12,13-Dibutyrate; D011493:Protein Kinase C