title: Antigen presentation by the CD4 positive monocyte subset.
abstract: Although CD4 antigen is expressed on monocytes (MO), its functional role is uncharacterized. In this study, isolated human MO were separated into CD4+ and CD4- MO subsets and assessed for presentation of tetanus toxoid. The CD4- MO subset had decreased antigen presenting cell (APC) capacity as well as increased PGE2 production when compared to the CD4+ MO subset. Addition of a cyclo-oxygenase inhibitor (Indomethacin) did not restore the CD4- MO subset's APC capacity to that of the similarly treated CD4+ MO subset, eliminating differential PGE2 production as the primary cause of differential APC capacity. Production of monokines such as IL-1 and plasminogen activator, which affect APC capacity, was similar in the CD4 MO subsets. However, tumor necrosis factor (TNF) production (IFN gamma plus MDP-induced) of the CD4+ MO subset was slightly greater than that of the CD4- MO. CD4- MO's lower APC capacity is not totally explained by their differential IL-1, TNF, or PGE2 production.
authors.affiliation: Department of Surgery, University of Massachusetts Medical Center, Worcester 01655.
authors.forename: G
authors.initials: G
authors.lastname: Szabo
authors.forename: C L
authors.initials: CL
authors.lastname: Miller
authors.forename: K
authors.initials: K
authors.lastname: Kodys
mesh_terms: D000328:Adult; D000938:Antigen-Presenting Cells / Q000276:immunology* / Q000378:metabolism; D015704:CD4 Antigens / Q000032:analysis*; D002469:Cell Separation / Q000379:methods; D015232:Dinoprostone / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism / Q000494:pharmacology; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism; D010960:Plasminogen Activators / Q000378:metabolism / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology; D013745:Tetanus Toxoid / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism / Q000494:pharmacology
chemical_list: D015704:CD4 Antigens; D007375:Interleukin-1; D013745:Tetanus Toxoid; D014409:Tumor Necrosis Factor-alpha; D010960:Plasminogen Activators; D015232:Dinoprostone