Determination of 7-dehydrocholesterol in human skin by high-performance liquid chromatography.
A two-stage chromatographic procedure has been devised for the measurement of 7-dehydrocholesterol in human skin. Extracts containing ergosterol as internal standard underwent preparative chromatography on a Spherisorb S5W column using hexane-1% isopropanol as solvent, and an eluted fraction was analysed with an Ultrasphere 5-microns ODS column with methanol-tetrahydrofuran-17.5 mM KH2PO4 (95:1:4, v/v) as solvent and using an amperometric detector at 1.7 V. 7-Dehydrocholesterol could be reliably assayed in human skin samples as small as 5 mm in diameter. In hospital patients skin 7-dehydrocholesterol concentrations ranged from 12 to 81 micrograms/g dry weight.
Moody
J P
JP
Department of Biochemical Medicine, Ninewells Hospital, Scotland, U.K.
Humphries
C A
CA
Allan
S M
SM
Paterson
C R
CR
D000375:Aging; D002784:Cholesterol / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003684:Dehydrocholesterols / Q000032:analysis*; D004875:Ergosterol / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D012016:Reference Values; D012867:Skin / Q000737:chemistry*
D003684:Dehydrocholesterols; D002784:Cholesterol; C016705:7-dehydrocholesterol; D004875:Ergosterol