title: Molecular cloning of a cDNA encoding the human interleukin 4 receptor.
abstract: Using the mouse interleukin 4 (IL-4) receptor cDNA as a probe, we isolated a cDNA encoding the human IL-4 receptor (hIL-4 receptor) from a multifactor-responsive human myeloid cell line, TF1. The cDNA encodes for an open reading frame of 825 amino acids including a signal sequence (25 amino acids), the external domain (207 amino acids), a transmembrane domain (24 amino acids), and a large cytoplasmic domain (569 amino acids). The human IL-4 receptor has a 65% identity with the mouse IL-4 receptor at the nucleic acid level and retains the typical structural motif of the previously described cytokine receptor family. COS7 cells transfected with the full-length cDNA expressed high levels (140,000 sites/cell) of IL-4 binding sites, with a Kd = 80 pM, an affinity identical to that of the original TF1 cells. Similar to IL-4 responsive cells, cross-linking of [125I]IL-4 to COS7 cells transfected with the cDNA showed a major protein of 130-150 kd and minor species of 55-85 kd.
authors.affiliation: Schering-Plough, Dardilly, France.
authors.forename: J P
authors.initials: JP
authors.lastname: Galizzi
authors.forename: C E
authors.initials: CE
authors.lastname: Zuber
authors.forename: N
authors.initials: N
authors.lastname: Harada
authors.forename: D M
authors.initials: DM
authors.lastname: Gorman
authors.forename: O
authors.initials: O
authors.lastname: Djossou
authors.forename: R
authors.initials: R
authors.lastname: Kastelein
authors.forename: J
authors.initials: J
authors.lastname: Banchereau
authors.forename: M
authors.initials: M
authors.lastname: Howard
authors.forename: A
authors.initials: A
authors.lastname: Miyajima
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D006801:Humans; D015847:Interleukin-4 / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D019948:Receptors, Interleukin-4; D011975:Receptors, Mitogen / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D015342:DNA Probes; D019948:Receptors, Interleukin-4; D011975:Receptors, Mitogen; D015847:Interleukin-4; D004247:DNA