title: Sizing of the Leptospira genome by pulsed-field agarose gel electrophoresis.
abstract: Pulsed-field gel electrophoresis allowed the determination of the size of the genome of Leptospira, a bacterium of the spirochete family. The three restriction enzymes, NotI (5'GC/GGCCGC), NheI (5'G/CTAGC), ApaI (5'-GGGCC/C) generated DNA fragments of suitable size. The results are compatible with a size of 5000 kb for the chromosome of both the pathogenic and the saprophytic species of Leptospira.
authors.affiliation: Unité des Leptospires, Institut Pasteur, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Baril
authors.forename: I
authors.initials: I
authors.lastname: Saint Girons
mesh_terms: D002876:Chromosomes, Bacterial; D015252:Deoxyribonucleases, Type II Site-Specific; D004587:Electrophoresis, Agar Gel; D005798:Genes, Bacterial; D007919:Leptospira / Q000235:genetics*; D007921:Leptospira interrogans / Q000235:genetics*
chemical_list: C067043:endodeoxyribonuclease NheI; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases; C038485:GGGCCC-specific type II deoxyribonucleases