Immunocytochemical localization of BP, a conceptus secretory protein, in trophectoderm during pig trophoblast elongation.
Tissue sections of periimplantation pig conceptuses (days 9-15 of pregnancy) were incubated with antiserum to the basic protein (BP), a major secretory protein of filamentous pig blastocysts. Bound antibody was detected by the peroxidase-antiperoxidase method. BP was restricted to trophectoderm in conceptuses which had made the transition from a spherical to an ovoid shape having a diameter of greater than 5 mm (day 11). Tubular (day 11, 10-20 mm) and filamentous (day 11-15) conceptus trophectoderm contained BP. These results suggest that BP synthesis commences at the time of rapid trophoblast growth.
Baumbach
G A
GA
Department of Animal Science, College of Veterinary Medicine, University of Tennessee, Knoxville 37901.
Bartley
N G
NG
Kattesh
H G
HG
Godkin
J D
JD
D000818:Animals; D001755:Blastocyst / Q000737:chemistry* / Q000648:ultrastructure; D004475:Ectoderm / Q000737:chemistry*; D005865:Gestational Age; D007124:Immunoenzyme Techniques; D011506:Proteins / Q000032:analysis*; D013552:Swine / Q000196:embryology*; D014327:Trophoblasts / Q000737:chemistry*
D011506:Proteins