[The identification of kinetochore proteins by using the sera of patients with collagenoses].
Sera of rheumatology patients with antikinetochore autoantibodies are identified. Immunoblot of human cell proteins showed that all the sera contained autoantibodies to kinetochore proteins A and B. In Chinese hamster cells proteins with m. m. 45 kD, 100 kD and 110 kD are presumably kinetochore proteins, whereas in murine cells these are proteins with m. m. 45 kD 85 kD and 100 kD.
Semenov
M V
MV
D000818:Animals; D001323:Autoantibodies / Q000097:blood; D002478:Cells, Cultured / Q000187:drug effects / Q000276:immunology; D002503:Centromere / Q000737:chemistry* / Q000187:drug effects / Q000276:immunology; D003095:Collagen Diseases / Q000276:immunology*; D006224:Cricetinae; D005455:Fluorescent Antibody Technique; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis* / Q000187:drug effects / Q000276:immunology; D012260:Ribonucleases / Q000494:pharmacology
D001323:Autoantibodies; D011506:Proteins; D012260:Ribonucleases
Vyiavlenie belkov kinetokhorov s pomoshch'iu syvorotok bol'nykh kollagenozami.