title: Binding of 125I-labelled albumin by isolated rat renal brush-border membrane vesicles. Evidence for uptake and internalization process.
abstract: 1. In the kidney, filtered proteins are rapidly reabsorbed by the proximal tubule via adsorptive endocytosis. This process starts with the protein binding to the luminal brush-border membrane. 2. The binding of 125I-labelled albumin to rat renal brush-border membrane vesicles and the effect of a low molecular weight protein lysozyme on that binding was assessed by the filtration method. 3. The Scatchard plot revealed a one-component binding-type curve with a dissociation constant Kd of 430.9 nM and 39.6 pmol/mg membrane protein for the number of binding sites. 4. Albumin binding was saturable and reversible, time and temperature dependent and the initial rate enhanced by increasing amounts of lysozyme. 5. The fact that association of albumin with the brush-border membrane vesicles was dependent upon the intravesicular space suggested a double process, binding of the ligand to the membrane surface and its internalization. These data suggest that albumin has a different binding site than that of a low-molecular weight protein lysozyme, with a constant affinity value near physiological loads. That specificity may confer selectivity upon the endocytic uptake process.
authors.affiliation: Laboratoire de Biologie cellulaire, Université Paris 7, France.
authors.forename: H
authors.initials: H
authors.lastname: Bakala
authors.forename: M
authors.initials: M
authors.lastname: Perichon
authors.forename: I
authors.initials: I
authors.lastname: Sudey
authors.forename: J
authors.initials: J
authors.lastname: Schaeverbeke
mesh_terms: D000818:Animals; D004705:Endocytosis; D006801:Humans; D007668:Kidney / Q000648:ultrastructure*; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism; D008871:Microvilli / Q000378:metabolism*; D009113:Muramidase / Q000494:pharmacology; D009994:Osmolar Concentration; D051381:Rats; D011919:Rats, Inbred Strains; D012711:Serum Albumin, Radio-Iodinated / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D012711:Serum Albumin, Radio-Iodinated; D009113:Muramidase