[Reorganization of chromatin superstructure during marked changes in the rate of protein synthesis].
The changes in the structure and RNA-polymerase activity of rat liver cell chromatin after a single injection of cycloheximide (3 mg/kg of body mass) were studied. The cycloheximide-induced fluctuations in protein synthesis rates are concomitant with episodes of drastic changes in the chromatin structure. The reorganization of the general protein structure is associated with an increase or a decrease of the RNA-polymerase II activity. The data obtained suggest that the activation-inactivation of RNA-polymerase II in cell nuclei is due to reorganization of chromatin infrastructures--from higher levels of the electron-dense chromatin package to the unfolded nucleosomes of the transcriptionally active protein.
Shevchenko
N A
NA
Boĭkov
P Ia
PIa
Ivanova
G I
GI
Todorov
I N
IN
D000818:Animals; D002843:Chromatin / Q000737:chemistry / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology; D004789:Enzyme Activation; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D046911:Macromolecular Substances; D008297:Male; D014176:Protein Biosynthesis; D011487:Protein Conformation; D012319:RNA Polymerase II / Q000037:antagonists & inhibitors / Q000378:metabolism; D051381:Rats
D002843:Chromatin; D046911:Macromolecular Substances; D003513:Cycloheximide; D012319:RNA Polymerase II
Reorganizatsiia superstruktur khromatina v usloviiakh rezkikh kolebaniĭ skorosti sinteza belkov.