Glutathione and ascorbic acid concentrations in the duodenum of rats with cysteamine-induced ulcers: influence of cysteine and ascorbic acid pretreatments.
Duodenal ulceration in rats was induced by a single subcutaneous injection of cysteamine at doses of 7, 28, 42 and 65 mg/100 g body weight 24 h before killing. Duodenal ulceration induced by cysteamine was dose-dependent. However, at 65 mg/100 g body weight, 5 of 6 animals died within 24 h. The concentrations of reduced glutathione (GSH) and ascorbic acid were measured in the duodenal homogenates of cysteamine-treated rats. The ulcerogen, at doses of 28 and 42 mg/100 g body weight, significantly reduced the GSH concentration. At a dose of 28 mg/100 g body weight, however, it did not significantly affect the duodenal ascorbic acid concentration. Pretreatment of rats with daily intramuscular injections of cysteine at 25, 50 and 100 mg/kg or ascorbic acid at 50, 100 and 200 mg/kg for 7 days had no significant effect on the duodenal ulceration produced by cysteamine (28 mg/100 g body weight), although each pretreatment significantly raised the duodenal concentrations of GSH and ascorbic acid respectively, in control rats, and to a lesser extent in cysteamine-treated animals.
Ali
B H
BH
Department of Pharmacology, Faculty of Medicine, Al-Arab Medical University, Benghazi, Libya.
Abdel Gayoum
A A
AA
Bashir
A A
AA
el-Fakhri
M
M
D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism* / Q000494:pharmacology; D003543:Cysteamine / Q000494:pharmacology; D003545:Cysteine / Q000494:pharmacology / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D004381:Duodenal Ulcer / Q000188:drug therapy / Q000378:metabolism*; D004386:Duodenum / Q000378:metabolism*; D005260:Female; D005978:Glutathione / Q000378:metabolism*; D011292:Premedication; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D003543:Cysteamine; D005978:Glutathione; D003545:Cysteine; D001205:Ascorbic Acid