[An approach to isolating individual tRNA from the human placenta by affinity chromatography. Isolation of highly purified Phe-tRNA Phe].
A new approach to isolation of individual tRNAs from eukaryotes based on affinity chromatography is suggested. At first, using a sorbent with oligonucleotide pTGGT attached, the total tRNA with native CCA-ends was obtained. Then by means of a sorbent with oligonucleotide pTTCAG immobilized, which is complementary to a part of the tRNA(Phe) anticodon loop, tRNA(Phe) with the acceptor activity greater than 1000 pmole/unit was isolated.
Zarytova
V F
VF
Karpova
G G
GG
Mundus
D A
DA
Shishkina
I G
IG
D002846:Chromatography, Affinity; D005260:Female; D006801:Humans; D010920:Placenta / Q000737:chemistry*; D012360:RNA, Transfer, Phe / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
D012360:RNA, Transfer, Phe
Podkhod k polucheniiu individual'nykh tRNK iz platsenty cheloveka, osnovannyĭ na metode affinnoĭ khromatografii. Vydelenie vysokoobogashchennogo preparata Phe-tRNK Phe.