title: Genetic recombination in human melanoma and astrocytoma cell lines involves oncogenes and growth factor genes.
abstract: We describe here the chromosomal distribution of sister chromatid exchanges (SCEs) in four human tumor cell lines (two melanomas and two astrocytomas), and have mapped the sister chromatid recombination (SCR) sites. A higher incidence of SCR sites than expected on the basis of chromosome length occurred in chromosomes 2, 4, 5 and 15 in both the RPMI 5966 and MEL57 melanoma cell lines, and in chromosomes 1, 5, 13 and 15 of the IJKt and GUVW astrocytoma cell lines. A majority of the recombination sites occurred close to chromosomal fragile sites. A third of these occurred at the same bands as fragile sites. The recombination sites involved the N-ras and the epidermal growth factor gene in the melanomas. In the astrocytomas, the N-ras, Rb and c-mos genes appeared to be involved in the recombination events. The beta 2-microglobulin gene was involved in both astrocytomas and one melanoma. The erbB2 was involved in SCR only in the RPMI melanoma.
authors.affiliation: Division of Oncology, University of Newcastle upon Tyne, Medical School, U.K.
authors.forename: M S
authors.initials: MS
authors.lastname: Lakshmi
authors.forename: G V
authors.initials: GV
authors.lastname: Sherbet
mesh_terms: D001254:Astrocytoma / Q000235:genetics*; D043283:Chromosome Fragile Sites; D002873:Chromosome Fragility; D002874:Chromosome Mapping; D002894:Chromosomes, Human, Pair 4; D005796:Genes; D006133:Growth Substances / Q000235:genetics*; D006801:Humans; D008545:Melanoma / Q000235:genetics*; D009857:Oncogenes; D011995:Recombination, Genetic; D012854:Sister Chromatid Exchange; D014407:Tumor Cells, Cultured / Q000502:physiology
chemical_list: D006133:Growth Substances