Novel human brain cDNA encoding a 34,000 Mr protein n-chimaerin, related to both the regulatory domain of protein kinase C and BCR, the product of the breakpoint cluster region gene.
A novel human brain complementary DNA sequence encodes n-chimaerin, a 34,000 Mr protein. A single cysteine-rich sequence CX2CX13CX2CX7CX7C in the N-terminal half of n-chimaerin shares almost 50% identity with corresponding sequences in the C1 regulatory domain of protein kinase C. The C-terminal half of n-chimaerin has 42% identity with the C-terminal region (amino acid residues 1050 to 1225) of BCR, the product of the breakpoint cluster region gene involved in Philadelphia (Ph') chromosome translocation. n-Chimaerin mRNA (2.2 x 10(3) base-pairs) is specifically expressed in the brain, with the highest amounts being in the hippocampus and cerebral cortex. The mRNA has a neuronal distribution and is expressed in neuroblastoma cells, but not in C6 glioma or primary astrocyte cultures. The similarity of two separate regions of n-chimaerin to domains of protein kinase C and BCR has intriguing implications with respect to its evolutionary origins, its function in the brain and potential phorbol-ester-binding properties.
Hall
C
C
Department of Neurochemistry, Institute of Neurology, London, U.K.
Monfries
C
C
Smith
P
P
Lim
H H
HH
Kozma
R
R
Ahmed
S
S
Vanniasingham
V
V
Leung
T
T
Lim
L
L
D000595:Amino Acid Sequence; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D020848:Chimerin 1; D004247:DNA / Q000235:genetics*; D015870:Gene Expression; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D005810:Multigene Family; D009419:Nerve Tissue Proteins / Q000235:genetics*; D010677:Philadelphia Chromosome; D011493:Protein Kinase C / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D020848:Chimerin 1; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D004247:DNA; D011493:Protein Kinase C