title: Immunocytological localization of the highly polysialylated form of the neural cell adhesion molecule during development of the murine cerebellar cortex.
abstract: The expression of the highly polysialylated form of the neural cell adhesion molecule (N-CAM)--the so-called embryonic N-CAM (E-N-CAM)--was investigated in the developing and adult mouse cerebellar cortex by immunohistology and immunocytology at the light and electron microscopic levels. E-N-CAM was never (from embryonic day 14 to postnatal day 15) detectable in the germinal zone of neuroblasts destined to form or forming the external granular layer and was only observed once small cerebellar interneurons had become postmitotic before the beginning of granule cell migration. Granule cells expressed E-N-CAM on cell bodies, axons, and leading and trailing processes also during migration but ceased to reveal detectable levels of E-N-CAM at the end of migration after having reached their final position in the internal granular layer. Other cerebellar cell types, such as Purkinje cells, Bergmann glia, astrocytes, oligodendrocytes, and most prominently, stellate and basket cells, also expressed E-N-CAM, but became E-N-CAM-negative during the third and fourth postnatal weeks, coinciding with overt cessation of cerebellar histogenesis. Thus, except for neuroblasts, E-N-CAM appeared characteristic of growing and moving cellular structures, in agreement with the notion that the highly polysialylated form of N-CAM is less adhesive than the adult form.
authors.affiliation: Department of Neurobiology, University of Heidelberg, Federal Republic of Germany.
authors.forename: A
authors.initials: A
authors.lastname: Hekmat
authors.forename: D
authors.initials: D
authors.lastname: Bitter-Suermann
authors.forename: M
authors.initials: M
authors.lastname: Schachner
mesh_terms: D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis*; D002525:Cerebellar Cortex / Q000032:analysis* / Q000254:growth & development / Q000648:ultrastructure; D051379:Mice / Q000254:growth & development*; D008854:Microscopy, Electron; D012794:Sialic Acids / Q000032:analysis*
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D012794:Sialic Acids; C021319:polysialic acid