Biological significance of enhanced mitochondrial ketogenesis during the early stages after 70% hepatectomy in rats.
Ketogenic capacity of mitochondria from the remnant liver of 70% hepatectomized rats was studied in relation to mitochondrial phosphorylative activity. Ketogenic capacity increased to a maximum of 6.04 +/- 0.39 from 3.84 +/- 0.13 of control, with an enhancement of mitochondrial phosphorylative activity 6 hr after hepatectomy, and then decreased to normal levels within 24 hr. Adenylate energy charge, (ATP + 1/2ADP)/(ATP + ADP + AMP), of the remnant liver decreased to 0.825 +/- 0.006 as compared to 0.849 +/- 0.002 of control 6 hr after operation. At 12 hr, total ketone body concentrations of the arterial blood increased concomitant with a fall in ketone body ratio (acetoacetate/3-hydroxybutyrate) which reflects the decreased liver mitochondrial redox (NAD+/NADH) state. These findings suggest that an enhancement of mitochondrial fatty acid oxidation and ketogenesis occurs concomitant with an enhancement of mitochondrial phosphorylative activity in the remnant liver in response to a decreased energy charge after 70% hepatectomy.
Taki
Y
Y
Department of Surgery, Kyoto University Medical School, Japan.
Morimoto
T
T
Tanaka
A
A
Noguchi
M
M
Wang
W Y
WY
Nishihira
T
T
Nishikawa
K
K
Mori
K
K
Ozawa
K
K
D000818:Animals; D001158:Arteries; D004734:Energy Metabolism; D006498:Hepatectomy / Q000379:methods*; D007657:Ketone Bodies / Q000096:biosynthesis* / Q000097:blood; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism*; D011184:Postoperative Period; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D007657:Ketone Bodies