[The effect of exogenous peroxidase on the intensity of luminol-dependent macrophage chemiluminescence].
The influence of exogenous horse-radish peroxidase on the capacity of mouse peritoneal macrophages to luminol-dependent chemiluminescence induced by zymosan was investigated. It was revealed that peroxidase (1-50 mg/ml) increased the chemiluminescence in a dose-dependent manner. The maximum increase of the response (4-6 times) was obtained with the enzyme concentration being 10 mg/ml. It is found that peroxidase acts as a co-oxidant of the peroxide-dependent extracellular luminol oxidation. The including of the enzyme into macrophages makes it possible to register the intracellular chemiluminescence.
Tokmakov
A A
AA
Vasil'ev
V Iu
VIu
D000818:Animals; D002374:Catalase / Q000494:pharmacology; D002474:Cell-Free System / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006735:Horseradish Peroxidase / Q000494:pharmacology*; D008163:Luminescent Measurements; D008165:Luminol / Q000378:metabolism*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010084:Oxidation-Reduction / Q000187:drug effects; D010529:Peritoneal Cavity / Q000166:cytology; D010587:Phagocytosis / Q000187:drug effects / Q000502:physiology; D015054:Zymosan / Q000494:pharmacology
D008165:Luminol; D015054:Zymosan; D006735:Horseradish Peroxidase; D002374:Catalase
Vliianie ékzogennoĭ peroksidazy na intensivnost' liuminolzavisimoĭ khemiliuminestsentsii makrofagov.