Expression of protein kinase CI in NIH 3T3 cells increases its growth response to specific activators.
In order to investigate the effects of protein kinase C (PKC) expression on cellular growth and morphology, we established mouse fibroblast cell populations which expressed the rat pkc-gamma gene under the control of a retroviral promoter. NIH 3T3 stable transfectants displayed a three-fold increase in total PKC levels. These cells appeared morphologically unaltered but exhibited a stronger mitogenic response to 12-O-tetradecanoylphorbol-13-acetate (TPA) and cardiolipin (CL) as well as enhanced growth in semisolid medium in the presence of TPA. Thus, at these enzyme levels, PKC conferred growth advantages to NIH 3T3 cells only in response to specific activators.
Cuadrado
A
A
Laboratory of Molecular and Cellular Biology, National Cancer Institute, Bethesda, MD 20892.
Molloy
C J
CJ
Pech
M
M
D000818:Animals; D002308:Cardiolipins / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D003001:Cloning, Molecular / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D005822:Genetic Vectors; D051379:Mice; D010957:Plasmids; D011493:Protein Kinase C / Q000235:genetics*; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014162:Transfection
D002308:Cardiolipins; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate