title: [Use of bacterial luciferase for determining monoamine oxidase activity].
abstract: A bioluminescence assay is proposed for measuring monoamine oxidase activity in different biological specimens (platelets, mitochondria). The assay is based on the bioluminescent reaction catalysed by bacterial luciferase and coupled to monoamine oxidase. Two modifications of the bioluminescence assay were used. In the first case, the bioluminescent system was added to monoamine oxidase preincubated with the substrates, while in the second case, all the components of the coupled enzymatic systems were directly mixed in a cell. The proposed bioluminescence assay is simple, highly sensitive and rapid, and could be especially useful for biomedical examinations.
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Sobolev
authors.forename: M M
authors.initials: MM
authors.lastname: Mazhul'
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Malkov
authors.forename: V S
authors.initials: VS
authors.lastname: Danilov
authors.forename: V Z
authors.initials: VZ
authors.lastname: Gorkin
authors.forename: T A
authors.initials: TA
authors.lastname: Moskvitina
authors.forename: L N
authors.initials: LN
authors.lastname: Ovchinnikova
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000201:enzymology; D001921:Brain / Q000201:enzymology; D002417:Cattle; D008156:Luciferases; D008163:Luminescent Measurements; D008928:Mitochondria / Q000201:enzymology; D008995:Monoamine Oxidase / Q000378:metabolism*; D014733:Vibrio / Q000201:enzymology*
chemical_list: D008156:Luciferases; D008995:Monoamine Oxidase
vernacular_title: Ispol'zovanie bakterial'noĭ liutsiferazy dlia opredeleniia monoaminooksidaznoĭ aktivnosti.