title: The sequences of two peptides from cataract lenses suggest they arise by deamidation.
abstract: Polyclonal antisera made against synthetic peptides corresponding to expected tryptic fragments of gamma crystallin have been used to screen tryptic digests of total proteins from cataractous versus normal human lenses. One of these antisera recognizes two peptides that were found in greater amounts from digests of cataractous lenses. These two peptides shared a common sequence that contained an aspartate residue in place of an expected asparagine, suggesting that increased deamidation of this residue had occurred in the human cataractous lens in vivo.
authors.affiliation: Division of Biology, Kansas State University, Manhattan 66506.
authors.forename: L
authors.initials: L
authors.lastname: Takemoto
authors.forename: T
authors.initials: T
authors.lastname: Emmons
authors.forename: D
authors.initials: D
authors.lastname: Granstrom
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D000818:Animals; D001216:Asparagine / Q000378:metabolism; D001224:Aspartic Acid / Q000378:metabolism; D002386:Cataract / Q000378:metabolism*; D002417:Cattle; D003459:Crystallins / Q000378:metabolism*; D003641:Deamination; D006801:Humans; D007908:Lens, Crystalline / Q000378:metabolism*; D008875:Middle Aged; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology; D010455:Peptides / Q000378:metabolism*; D011817:Rabbits; D011863:Radioimmunoassay; D012689:Sequence Homology, Nucleic Acid; D014357:Trypsin
chemical_list: D003459:Crystallins; D010446:Peptide Fragments; D010455:Peptides; D001224:Aspartic Acid; D001216:Asparagine; D014357:Trypsin