Isolation of TSH and LH/CG receptor cDNAs from human thyroid: regulation by tissue specific splicing.
A TSH receptor (TSH-R) cDNA has been isolated from a human thyroid lambda GT11 library. Unexpectedly, several cDNAs encoding the human LH/CG receptor (LH/CG-R), previously thought to be expressed solely in gonadal cells, were also isolated from the thyroid library. The receptors are structurally related, consisting of a signal sequence, a large extracellular amino terminal domain, seven membrane spanning domains, and a short carboxyl-terminal portion. The TSH-R is encoded by a single 4.2 kilobase mRNA specific to the thyroid. Introns were not present in any hTSH-R cDNAs examined, however, sequencing of several LH/CG-R cDNAs and RNase protection experiments demonstrated that the majority of hLH/CG-R mRNA in the thyroid is incompletely spliced. Consequently, tissue-specific splicing may be an important step in the regulation of the glycoprotein hormone receptor family.
Frazier
A L
AL
Department of Pathology, New England Medical Center Hospital, Boston, Massachusetts 02111.
Robbins
L S
LS
Stork
P J
PJ
Sprengel
R
R
Segaloff
D L
DL
Cone
R D
RD
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D005091:Exons; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D007438:Introns; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D011974:Receptors, LH / Q000235:genetics*; D011989:Receptors, Thyrotropin / Q000235:genetics*; D012260:Ribonucleases; D012689:Sequence Homology, Nucleic Acid; D013961:Thyroid Gland / Q000737:chemistry*
D011974:Receptors, LH; D011989:Receptors, Thyrotropin; D004247:DNA; D012260:Ribonucleases