Endoneurial microenvironment and acute nerve crush injury in the rat sciatic nerve.
In severe peripheral nerve ischemia in the rat, serial nerve blood flow (NBF) measurements have identified evidence of 'no reflow', a mechanism of continued fiber damage during reperfusion. It has been postulated that 'no reflow' also occurs in nerve compression due to direct mechanical or ischemic (if compression is prolonged) injury of microvessels, resulting in continuing nerve fiber damage. To address this question, we measured endoneurial blood flow (NBF), oxygen tension and pH at the site of an acute nerve crush injury. In further sets of experiments, NBF and endoneurial oxygen tension were examined before and after prolonged epochs of crush. NBF and MR (microvascular resistance) were not appreciably different than values obtained in control animals without intervening brief nerve crush. NBF was slightly higher and MR slightly lower 2 h after injury, but the difference was not statistically significant. No evidence of significant endoneurial hypoxia or acidosis was observed. Similarly, after more prolonged crush there was no significant oligemia or hypoxia. The studies provide no evidence that 'no reflow' occurs in crush injury even if the injury is maintained for a period of time known to induce 'no reflow' with severe ischemia. We suggest that nerve damage in crush, and possibly compression, more likely arises from direct mechanical injury of fibers.
Zochodne
D W
DW
Department of Medicine, Queen's University, Kingston, Ont., Canada.
Ho
L T
LT
D000818:Animals; D003238:Connective Tissue / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007511:Ischemia / Q000209:etiology / Q000503:physiopathology; D008297:Male; D008833:Microcirculation; D008839:Microelectrodes; D009408:Nerve Compression Syndromes / Q000150:complications / Q000503:physiopathology; D009409:Nerve Crush; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012039:Regional Blood Flow; D012584:Sciatic Nerve / Q000098:blood supply / Q000293:injuries*; D014655:Vascular Resistance / Q000502:physiology
D010100:Oxygen