Quantitative estimation of major histocompatibility complex antigens on live tumour cells.
Quantitation of major histocompatibility complex (MHC) antigens on cells can accurately be done by using a flowcytometer. Since flowcytometer is not freely accessable an alternate, simple method for relative quantitation of MHC antigens has been devised. In this procedure, YAC lymphoma cells were first treated with a monoclonal anticlass I MHC antibody and then with a rabbit anti mouse Ig-antibody coupled to peroxidase, followed by colour development using a substrate of peroxidase enzyme. Various assay parameters have been optimized. The validity of the procedure was examined by assessing the enhanced MHC expression on YAC cells treated with a soluble rat spleen derived factor, by the new procedure as well as by the flowcytometer. Comparable results were obtained by using both techniques.
Sarin
A
A
Immunology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Saxena
R K
RK
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D014407:Tumor Cells, Cultured / Q000276:immunology*
D015395:Histocompatibility Antigens Class I