title: Immunomodulation of vascular endothelium: effects of ultraviolet B irradiation on vein allograft rejection.
abstract: Prosthetic grafts of vein allografts are inadequate as small-diameter vessel substitutes. We have applied ultraviolet B (UVB) irradiation to modulate the immunogenicity of vein allografts to avoid immunologic injury. The veins of male ACI rats were irradiated with UVB (60 mJ/cm2) in situ and transplanted to male ACI rats (autografts) and female Lewis rats (allografts). Nonirradiated veins served as controls. At 4, 7, 14, and 28 days, all grafts were patent and were studied for morphologic changes by scanning electron microscopy and for immunogold labeling of major histocompatibility complex class II antigen expression. In autografts, scanning electron microscopy demonstrated minimal endothelial loss after grafting, regardless of UVB irradiation. Untreated allografts showed severe endothelial injury 4, 7, and 14 days after transplantation. UVB irradiation of veins protected allografts from injury to the endothelium and basement membrane. Major histocompatibility complex class II-positive endothelial cells were not seen in autografts but were seen in 40% of cells 4 days after transplantation in untreated allografts. UVB-treated allografts showed MHC class II antigen expression labeling of 20% of the endothelial cells. Barr body analysis demonstrated the donor origin of these endothelial cells. UVB irradiation of rat vein allografts prolongs endothelial survival while decreasing endothelial surface expression of class II antigens. These data suggest that modification of vein immunogenicity with UVB irradiation may permit functional survival of small-vessel allografts without chronic immunosuppression.
authors.affiliation: Department of Surgery, Columbia University College of Physicians and Surgeons, New York, NY 10032.
authors.forename: M L
authors.initials: ML
authors.lastname: Marin
authors.forename: M A
authors.initials: MA
authors.lastname: Hardy
authors.forename: R E
authors.initials: RE
authors.lastname: Gordon
authors.forename: K
authors.initials: K
authors.lastname: Reemtsma
authors.forename: A I
authors.initials: AI
authors.lastname: Benvenisty
mesh_terms: D000005:Abdomen; D000818:Animals; D004730:Endothelium, Vascular / Q000276:immunology* / Q000528:radiation effects / Q000648:ultrastructure; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D011912:Rats, Inbred ACI; D011917:Rats, Inbred Lew; D014466:Ultraviolet Rays; D014680:Veins / Q000166:cytology / Q000528:radiation effects / Q000637:transplantation* / Q000648:ultrastructure
chemical_list: D000949:Histocompatibility Antigens Class II