PGD phenotyping in bloodstains, organ tissues, dental pulps and hair roots by isoelectric focusing.
Polymorphism of PGD was investigated in bloodstains, organ tissues, dental pulps, hair roots and semen by isoelectric focusing. This technique provided much higher resolution of PGD isoenzymes than starch gel electrophoresis. Phenotyping was possible from bloodstains for 5 weeks, from organ tissues (except pancreas) for 1-3 weeks, from dental pulps for 2 weeks and from hair roots for 2 weeks when they were stored at room temperature. The method is simple, rapid, reliable and therefore useful in medicolegal individualization of bloodstains, organ tissues, teeth and hairs.
Komatsu
N
N
Department of Legal Medicine, Yamanashi Medical University, Japan.
Kido
A
A
Kimura
Y
Y
Oya
M
M
D001801:Blood Stains; D003782:Dental Pulp / Q000737:chemistry*; D005260:Female; D006197:Hair / Q000737:chemistry*; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis*; D008297:Male; D010641:Phenotype; D010734:Phosphogluconate Dehydrogenase / Q000032:analysis*; D011309:Preservation, Biological
D007527:Isoenzymes; D010734:Phosphogluconate Dehydrogenase