Heparin modulation of laminin polymerization.
Previously, it has been shown that laminin will self-assemble by a two-step calcium-dependent process using end-domain interactions (Yurchenco, P. D., Tsi-library, E. C., Charonis, A. S., and Furthmayr, H. (1985) J. Biol. Chem. 260, 7636-7644). We now find that heparin, at low concentrations, modifies this polymerization by driving the equilibrium further toward aggregation, by producing a denser polymer, and by inducing aggregation in the absence of calcium. This effect on self-assembly is specific in that it is observed with heparin but not with several heparan sulfates or other glycosaminoglycans: it correlates with affinity and depends on the degree of polysaccharide sulfation. Heparin binds to laminin in a calcium-dependent manner with a single class of interaction (KD = 118 +/- 18 nM) and with a binding capacity of one heparin for two laminins. We find the long arm globule (E3) is the only laminin domain which exhibits substantial heparin binding: heparin binds E3 with an affinity (KD = 94 +/- 12 nM) and calcium dependence similar to that for intact laminin. These data strongly suggest that heparin modifies laminin assembly by binding to pairs of long arm globular domains. As a result the polymer may be stabilized at domain E3 and laminin interdomain interactions induced or modified. We further postulate that heparins may act in vivo as specific regulators of the structure and functions of basement membranes by both altering the laminin matrix and by displacing weakly binding heparan sulfates.
Yurchenco
P D
PD
Department of Pathology, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854.
Cheng
Y S
YS
Schittny
J C
JC
D000818:Animals; D001485:Basement Membrane / Q000378:metabolism; D002118:Calcium / Q000494:pharmacology; D002460:Cell Line; D006493:Heparin / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D007413:Intestinal Mucosa; D007700:Kinetics; D007797:Laminin / Q000302:isolation & purification / Q000378:metabolism* / Q000648:ultrastructure; D046911:Macromolecular Substances; D008970:Molecular Weight; D010446:Peptide Fragments / Q000302:isolation & purification; D011485:Protein Binding; D013552:Swine; D013816:Thermodynamics; D014407:Tumor Cells, Cultured / Q000378:metabolism
D007797:Laminin; D046911:Macromolecular Substances; D010446:Peptide Fragments; D006493:Heparin; D002118:Calcium