title: Removal of artifactual bands associated with the presence of 2-mercaptoethanol in two-dimensional polyacrylamide gel electrophoresis.
abstract: A method for eliminating artifactual bands due to the presence of 2-mercaptoethanol in two-dimensional gels is described. The method is based on a modification of the procedure of application of the first dimension gel to the SDS slab gel. 2-Mercaptoethanol is removed during equilibration and replaced by iodoacetamide. The use of iodoacetamide improves the recovery of proteins and results in a better detection of them.
authors.affiliation: Department of Genetics, Developmental and Molecular Biology, School of Sciences, University of Thessaloniki, Greece.
authors.forename: A
authors.initials: A
authors.lastname: Beis
authors.forename: A
authors.initials: A
authors.lastname: Lazou
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D008565:Membrane Proteins / Q000032:analysis; D008623:Mercaptoethanol / Q000032:analysis* / Q000737:chemistry; D009865:Oocytes / Q000648:ultrastructure; D011895:Rana ridibunda; D012834:Silver; D012967:Sodium Dodecyl Sulfate
chemical_list: D008565:Membrane Proteins; D012967:Sodium Dodecyl Sulfate; D012834:Silver; D008623:Mercaptoethanol