[Localization and activity of different lysosomal proteases in rat macrophages infected by Leishmania amazonensis].
Leishmania are protozoans of the trypanosomatidae family that cause human infections. The amastigote form of Leishmania is an obligate intracellular parasite of mononuclear phagocytes that multiplies within parasitophorous vacuoles (pv) of phagolysosomal origin. To investigate the strategies which allow Leishmania to withstand these potentially cytotoxic conditions, the distribution and activities of various lysosomal peptidases in rat macrophages infected or uninfected with Leishmania amazonensis amastigotes were studied. Specific immunoglobulins against cathepsins (cat.) B, H, L and D were used to localize these endopeptidases by immunocytochemistry. Results showed that most or even all of the secondary lysosomes in the host cell fuse with parasite-filled phagosomes, leading to translocation of the proteases in the parasitophorous vacuoles. A further study consisted in assays of five protease activities: dipeptidylpeptidases (DPP) I and II (exopeptidases), cat. B, cat. H and cat. D. Infection of macrophages was followed by a gradual increase in all these protease activities except for DPP II. These increases were apparently not related to parasite protease activities. It seems that infection by Leishmania amazonensis is followed by increased synthesis and/or reduced catabolism of host cell lysosomal proteases or alternatively by inactivation of endogenous inhibitors. Amastigote infectivity may be related, at least in part, to the development of mechanisms that allow the parasite to withstand unfavorable environmental conditions.
Prina
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Unité d'Immunophysiologie Cellulaire, l'Institut Pasteur, Paris, France.
Antoine
J C
JC
D000818:Animals; D010450:Endopeptidases / Q000378:metabolism*; D007891:Leishmania / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology*; D008264:Macrophages / Q000469:parasitology* / Q000648:ultrastructure; D051381:Rats
D010450:Endopeptidases
Localisation et activité de différentes protéases lysosomales dans des macrophages de rat infectés par Leishmania amazonensis.