Two distinct ferredoxins from Rhodobacter capsulatus: complete amino acid sequences and molecular evolution.
Two distinct ferredoxins were purified from Rhodobacter capsulatus SB1003. Their complete amino acid sequences were determined by a combination of protease digestion, BrCN cleavage and Edman degradation. Ferredoxins I and II were composed of 64 and 111 amino acids, respectively, with molecular weights of 6,728 and 12,549 excluding iron and sulfur atoms. Both contained two Cys clusters in their amino acid sequences. The first cluster of ferredoxin I and the second cluster of ferredoxin II had a sequence, CxxCxxCxxxCP, in common with the ferredoxins found in Clostridia. The second cluster of ferredoxin I had a sequence, CxxCxxxxxxxxCxxxCM, with extra amino acids between the second and third Cys, which has been reported for other photosynthetic bacterial ferredoxins and putative ferredoxins (nif-gene products) from nitrogen-fixing bacteria, and with a unique occurrence of Met. The first cluster of ferredoxin II had a CxxCxxxxCxxxCP sequence, with two additional amino acids between the second and third Cys, a characteristics feature of Azotobacter-[3Fe-4S] [4Fe-4S]-ferredoxin. Ferredoxin II was also similar to Azotobacter-type ferredoxins with an extended carboxyl (C-) terminal sequence compared to the common Clostridium-type. The evolutionary relationship of the two together with a putative one recently found to be encoded in nifENXQ region in this bacterium [Moreno-Vivian et al. (1989) J. Bacteriol. 171, 2591-2598] is discussed.
Saeki
K
K
Department of Biology, Faculty of Science, Osaka University.
Suetsugu
Y
Y
Yao
Y
Y
Horio
T
T
Marrs
B L
BL
Matsubara
H
H
D000595:Amino Acid Sequence; D005075:Biological Evolution; D003488:Cyanogen Bromide; D010450:Endopeptidases; D005288:Ferredoxins / Q000235:genetics*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation / Q000235:genetics; D016354:Rhodobacter capsulatus / Q000235:genetics*
D005288:Ferredoxins; D010450:Endopeptidases; D003488:Cyanogen Bromide