Immunoaffinity purification and characterisation of p29--an estrogen receptor related protein.
p29, a 29 kDa protein recognised by D5, a monoclonal antibody prepared against partially purified cytosolic estrogen receptor (ER), has been purified to homogeneity from ZR-75-1, a human breast cancer cell line. Ammonium sulphate fractionation followed by immunoaffinity chromatography on a three column system using Protein A-Sepharose coupled D5, produced purified p29. Silver stained SDS one-dimensional polyacrylamide gel electrophoresis (PAGE) and two-dimensional PAGE showed p29 to have been purified to homogeneity. Amino acid analysis showed no unusual characteristics. Partial N-terminal sequencing studies showed that purified p29 shared a 100% homology with the sequence of a pp89, murine cytomegaloviral protein.
Hayward
J R
JR
Roche Products Ltd, Welwyn Garden City, Hertfordshire, England.
Coffer
A I
AI
King
R J
RJ
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000645:Ammonium Sulfate; D001943:Breast Neoplasms / Q000737:chemistry; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005590:Fractional Precipitation; D006360:Heat-Shock Proteins; D006801:Humans; D007118:Immunoassay; D008969:Molecular Sequence Data; D009215:Myometrium / Q000737:chemistry; D009363:Neoplasm Proteins / Q000302:isolation & purification; D010750:Phosphoproteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D011960:Receptors, Estrogen; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured
D000596:Amino Acids; D006360:Heat-Shock Proteins; D009363:Neoplasm Proteins; D010750:Phosphoproteins; D011960:Receptors, Estrogen; D000645:Ammonium Sulfate