Incidence and specificity of HLA-DP antibodies in pregnancy sera.
A total of 630 human pregnancy sera were investigated for HLA-DP antibodies (ab) by monoclonal antibody-specific immobilization of leukocyte antigens (MAILA) using monoclonal antibody (mAB) B7/21.2 and selected B-lymphoblastoid cell lines from the Tenth International Histocompatibility Workshop reference panel. DP-specific abs were detected in 86 of 330 sera (26.1%) of a retrospective series selected for positive reactions in lymphocytotoxicity screening, and in 29 of 300 unselected sera (9.7%) of a prospective series. Approximately 80% of DP-reactive sera were also positive in lymphocytotoxicity test for class I and/or class II ab. On the other hand, sera containing lymphocytotoxic ab for both class I and class II ab revealed the highest incidence of DP ab (35%). Out of 115 DP-reactive sera, 28 clearly presented one or more DP specificities. Absorption/elution studies revealed complex patterns of reactivity in some sera which were similar to those of known mABs and possibly reflect supertypic DP specificities and/or serological cross-reactions. Serological DP typing of individuals by the MAILA technique appears promising.
Mueller-Eckhardt
G
G
Institute for Clinical Immunology and Transfusion Medicine, Justus Liebig University Giessen, Federal Republic of Germany.
Kiefel
V
V
Tlusty
A
A
Scholten
J
J
Schmidt
A
A
Mueller-Eckhardt
C
C
D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology*; D002461:Cell Line, Transformed; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D006682:HLA-DP Antigens / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D011247:Pregnancy / Q000276:immunology*; D015203:Reproducibility of Results
D000911:Antibodies, Monoclonal; D006682:HLA-DP Antigens; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II