Tumor necrosis factor and wound healing.
Tumor necrosis factor alpha (TNF), 1 to 500 ng in saline (PBS) or collagen, was applied to the wounds of normal and Adriamycin-impaired mice and wound disruption strength (WDS) and histology were examined. Also wounded mice were administered TNF 25 to 75 micrograms/kg IP daily and WDS was determined. Wound histology was examined 6 months after wounding and local TNF application. Local TNF 5 to 500 ng in PBS did not significantly affect WDS. Local TNF 5 to 50 ng in collagen increased WDS 33% to 65% in Adriamycin-impaired animals (p = 0.05 to p less than 0.02). Local TNF 50 to 500 ng in collagen increased WDS 23% to 49% in normal animals (p = 0.08 to p less than 0.01). Adriamycin-impaired animals demonstrated improved wound histology with local TNF in collagen. Systemic TNF did not significantly affect WDS. Local TNF in collagen did not induce histologic pathology at 6 months. TNF may modulate macrophage function and local TNF in collagen can improve WDS in normal and Adriamycin-impaired animals.
Mooney
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DP
Department of Surgery, University of Vermont, Burlington 05401.
O'Reilly
M
M
Gamelli
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RL
D000287:Administration, Topical; D000818:Animals; D003094:Collagen / Q000008:administration & dosage; D004317:Doxorubicin / Q000494:pharmacology; D006097:Granulation Tissue / Q000166:cytology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011897:Random Allocation; D012867:Skin / Q000293:injuries; D012965:Sodium Chloride / Q000008:administration & dosage; D014409:Tumor Necrosis Factor-alpha / Q000008:administration & dosage / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*
D014409:Tumor Necrosis Factor-alpha; D012965:Sodium Chloride; D004317:Doxorubicin; D003094:Collagen