title: Pneumonia in mice produced by cell-free extract of cultures of Bordetella parapertussis.
abstract: A single dose of culture fluid of Bordetella parapertussis freed from cells (CFCF) given intranasally to four-week-old mice free from intercurrent respiratory disease produced a subacute bronchopneumonia, which was similar to that induced by whole cells of ovine isolates of B parapertussis, except that the lesions were less severe and less extensive. From eight hours to 17 days after inoculation, the mice exhibited marked infiltration of neutrophils and macrophages into the alveolar septa, bronchiolar and alveolar spaces, and hyperplasia of peribronchiolar and perivascular lymphoid tissue. Electron microscopy showed damage to ciliated cells, type 1 pneumocytes and alveolar macrophages. These results suggest that extracellular toxic substance(s) produced by ovine isolates of B parapertussis might be involved in the initiation and development of lesions in ovine chronic non-progressive pneumonia.
authors.affiliation: Department of Veterinary Pathology, Massey University, Palmerston North, New Zealand.
authors.forename: W
authors.initials: W
authors.lastname: Chen
authors.forename: M R
authors.initials: MR
authors.lastname: Alley
authors.forename: B W
authors.initials: BW
authors.lastname: Manktelow
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000633:toxicity*; D001884:Bordetella; D001885:Bordetella Infections / Q000209:etiology / Q000473:pathology / Q000662:veterinary*; D001996:Bronchopneumonia / Q000209:etiology / Q000473:pathology / Q000662:veterinary*; D008168:Lung / Q000473:pathology / Q000648:ultrastructure; D051379:Mice; D011897:Random Allocation; D012756:Sheep; D012757:Sheep Diseases / Q000209:etiology* / Q000473:pathology
chemical_list: D001427:Bacterial Toxins