title: [Construction of a complete "library" of mutants at region -35 of a model prokaryotic promotor].
abstract: A novel approach to study the variability of consensus sites of regulatory regions of DNA is proposed. The overall strategy includes chemical synthesis of representative series of all possible DNA structures under investigation, cloning and screening according to their function. The chemical-enzymatic synthesis of a complete library of 40-bp DNA duplexes, corresponding to the model prokaryotic promoter and differing in 6-membered segments at -35 region, is described.
authors.forename: V L
authors.initials: VL
authors.lastname: Drutsa
authors.forename: A V
authors.initials: AV
authors.lastname: Krivonos
authors.forename: O N
authors.initials: ON
authors.lastname: Koroleva
authors.forename: Z A
authors.initials: ZA
authors.lastname: Shabarova
mesh_terms: D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D015723:Gene Library; D008969:Molecular Sequence Data; D009154:Mutation; D009692:Nucleic Acid Heteroduplexes; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D009692:Nucleic Acid Heteroduplexes; D004247:DNA
vernacular_title: Poluchenie polnoĭ "biblioteki" mutantov oblasti -35 model'nogo prokaryioticheskogo promotora.