Determination of lipoperoxide in animal tissue by ion-pair reversed phase high performance liquid chromatography.
An ion-pair reversed phase high performance liquid chromatographic method was developed for the quantitative determination of lipoperoxide (as malondialdehyde, MDA) in animal tissue using a ODS column and a mobile phase of methanol + phosphate buffer (53:47 v/v, pH 6.5). Tetrabutylammonium bromide was used as an ion-pair reagent and the detection wavelength was set at 532 nm. The method was sensitive, accurate and simple. A linear relationship was obtained between the peak height and the amount of MDA from 50 nmol/L to 1200 nmol/L and the lower limit of detection of MDA was 15 nmol/L (signal-to-noise ratio greater than 2), and the coefficient of variation at the 200 nmol/L level was 3.7% (n = 7). The results correlated well with those from the 2-thiobarbituric acid colorimetric method. The method has been used in the pathogenic studies of cerebral ischemia and acute renal failure for the determination of trace lipoperoxide.
Li
K
K
Department of Instrumental Analysis, Jinling Hospital, Nanjing, People's Republic of China.
D058186:Acute Kidney Injury / Q000378:metabolism; D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D003124:Colorimetry; D006868:Hydrolysis; D007202:Indicators and Reagents; D007668:Kidney / Q000737:chemistry; D008054:Lipid Peroxides / Q000032:analysis*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D013858:Thiobarbiturates; D014020:Tissue Extracts / Q000032:analysis
D007202:Indicators and Reagents; D008054:Lipid Peroxides; D013858:Thiobarbiturates; D014020:Tissue Extracts; D008315:Malondialdehyde