title: Detection of estrogen receptors in the trigonum and urinary bladder with an immunohistochemical technique.
abstract: In order to detect estrogen receptors (ER) in the female bladder, 5 premenopausal and 10 postmenopausal women affected by gynecological diseases were submitted to cystoscopy, during which both the trigonum and the bladder lateral wall were biopsied. A new, simple, cost-effective immunohistochemical technique was employed to stain the estrogen specific binding sites. ER were found in the trigonum of 3 premenopausal and 4 postmenopausal subjects, while the bladder lateral wall was always ER-negative. A comparison with previously used ER detection methods and a discussion of further hypothetical applications of the immunohistochemical technique in the study of the lower female urogenital tract are reported.
authors.affiliation: Department of Biomedical Sciences and Human Oncology, University of Turin, Ospedale Mauriziano Umberto I, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Bussolati
authors.forename: A
authors.initials: A
authors.lastname: Tizzani
authors.forename: G
authors.initials: G
authors.lastname: Casetta
authors.forename: P
authors.initials: P
authors.lastname: Cassoni
authors.forename: D
authors.initials: D
authors.lastname: Pacchioni
authors.forename: P
authors.initials: P
authors.lastname: Piana
authors.forename: A
authors.initials: A
authors.lastname: Revelli
authors.forename: M
authors.initials: M
authors.lastname: Massobrio
mesh_terms: D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D004967:Estrogens / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D011960:Receptors, Estrogen / Q000378:metabolism*; D001743:Urinary Bladder / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D004967:Estrogens; D011960:Receptors, Estrogen