Extracellular DNA in blood and urine as a potential marker for cytotoxicity and nephrotoxicity in the mouse.
Cell death releases products of chromatin catabolism, particularly DNA, which can be measured by a DNA assay (using the nick translation reaction) as a marker of toxicity. Conditions for blood and urine collection have been established for quantitation of extracellular DNA in mice. Toxic doses of lipopolysaccharide (5 mg/kg) and HgCl2 (3.2 mg/kg) release DNA to plasma and urine. Increase in urinary DNA is observed 24 hours after a single injection (5 mg/kg) of gentamicin. Quantitation of extracellular DNA can be used to investigate the occurrence of cell death in vivo and applied to toxicological studies, in animals and man.
Bret
L
L
Laboratoire d'Immunopathologie, Faculté de Médecine Purpan, Toulouse,France.
Lulé
J
J
Pourrat
J P
JP
Fournié
G J
GJ
D000818:Animals; D015415:Biomarkers; D002470:Cell Survival / Q000502:physiology; D004247:DNA / Q000032:analysis* / Q000097:blood / Q000652:urine; D005110:Extracellular Space; D005260:Female; D005839:Gentamicins / Q000633:toxicity; D007674:Kidney Diseases / Q000097:blood / Q000139:chemically induced* / Q000652:urine; D008070:Lipopolysaccharides / Q000633:toxicity; D008297:Male; D008627:Mercuric Chloride / Q000633:toxicity; D051379:Mice; D014116:Toxicology / Q000379:methods*
D015415:Biomarkers; D005839:Gentamicins; D008070:Lipopolysaccharides; D008627:Mercuric Chloride; D004247:DNA