title: Unequal crossing-over and gene conversion at the amplified CUP1 locus of yeast.
abstract: Meiotic recombination was analyzed between two twelve-copy arrays of a gene amplification at the CUP1 locus of Saccharomyces cerevisiae. Utilizing Southern analysis to identify spores with non-parental repeat arrays, we find that approximately 11% of a sample with 202 unselected tetrads possess at least one nonparental spore array. Both reciprocal and non-reciprocal changes are observed. The data suggest a model in which frequent mispairing among identical copies of the 2.0 kb repeat unit leads to the formation of unpaired loops containing integral numbers of repeat units. In this model, conversions involving the loops lead to non-reciprocal changes in arrays: about half are associated with reciprocal exchange, and net increases in repeat unit numbers occur about as frequently as net decreases. Thus, the known properties of gene conversion can account for all the segregations we observe.
authors.affiliation: Department of Plant Biology, University of California, Berkeley 94720.
authors.forename: J W
authors.initials: JW
authors.lastname: Welch
authors.forename: D H
authors.initials: DH
authors.lastname: Maloney
authors.forename: S
authors.initials: S
authors.lastname: Fogel
mesh_terms: D015139:Blotting, Southern; D003434:Crossing Over, Genetic; D004271:DNA, Fungal; D005784:Gene Amplification; D005785:Gene Conversion; D008957:Models, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013172:Spores, Fungal / Q000235:genetics
chemical_list: D004271:DNA, Fungal