Kinetic modeling of intracellular pH and comparison with 31P NMR experimental values in dialysed uremic patients.
Changes in intra-erythrocytic pH values over time, during and after bicarbonate hemodialysis, were studied with 31P Nuclear Magnetic Resonance. Simultaneously, pH values of whole blood were obtained by a gazometric method. A two-compartment model appeared to be the simplest kinetic model to explain the shifts in proton concentrations in extra- and intra-cellular media. Non-linear regression was used to determine exchange constant values. There was a very good correlation between the experimental and calculated proton concentrations. This model can describe all patients but individual experimental constants must be determined. Under these conditions a single blood pH determination before dialysis will permit determination of the initial intra-erythrocytic pH and monitoring of intra-erythrocytic pH during hemodialysis.
Monti
J P
JP
Laboratory of Biophysics, Faculty de Pharmacy, Marseille, France.
Sarrazin
M
M
Gallice
P
P
Crevat
A
A
Baz
M
M
Murisasco
A
A
Elsen
R
R
D000136:Acid-Base Equilibrium / Q000502:physiology*; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008962:Models, Theoretical; D006435:Renal Dialysis; D014511:Uremia / Q000097:blood* / Q000628:therapy