title: Preparation and antitumor activity of hydroxyethylated derivatives of 6-branched (1----3)-beta-D-glucan, SSG, obtained from the culture filtrate of Sclerotinia sclerotiorum IFO 9395.
abstract: SSG is an antitumor branched (1----3)-beta-D-glucan obtained from the culture filtrate of Sclerotinia sclerotiorum IFO 9395. Hydroxyethylation of SSG higher than MS 0.45 (MS value represents molar ratio of hydroxyethyl group vs. glucosyl group) by ethyleneoxide in aqueous sodium hydroxide lose the antitumor activity. Degradation of branching point of hydroxyethylated SSG (HE-SSG) by the sequential treatments of periodate oxidation, borohydride reduction, and mild acid hydrolysis of these derivatives regenerated the antitumor activity. These results directly demonstrated that the branching point covered, at least a part of, the dormant active site of SSG.
authors.affiliation: Lab. Immunopharmacology of Microbial Products, Tokyo College of Pharmacy, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kurachi
authors.forename: N
authors.initials: N
authors.lastname: Ohno
authors.forename: T
authors.initials: T
authors.lastname: Yadomae
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001203:Ascomycota / Q000032:analysis; D003470:Culture Media / Q000032:analysis; D005936:Glucans / Q000302:isolation & purification* / Q000494:pharmacology; D051379:Mice; D008813:Mice, Inbred ICR; D047071:beta-Glucans
chemical_list: D000970:Antineoplastic Agents; D003470:Culture Media; D005936:Glucans; D047071:beta-Glucans; C033363:beta-1,3-glucan