Determination of benzimidazole anthelmintics in meat samples.
A procedure for the detection of eight benzimidazole anthelmintics in meat samples using high-performance liquid chromatography is described. The limits of detection are in the range 20-50 micrograms/kg with a recovery of 66-87%. Chromatography is performed on an octadecylsilane column using mobile phases of acetonitrile-water with an ion-pair reagent, with UV detection. For verification of positive results, the drug substances are derivatized to methyl or pentafluorobenzyl derivatives suitable for detection by gas chromatography-mass spectrometry in the electron-impact or positive or negative chemical-ionization mode.
Marti
A M
AM
Swiss Federal Veterinary Office, Berne.
Mooser
A E
AE
Koch
H
H
D000818:Animals; D000871:Anthelmintics / Q000032:analysis* / Q000097:blood; D001562:Benzimidazoles / Q000032:analysis* / Q000097:blood; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination; D008401:Gas Chromatography-Mass Spectrometry; D008460:Meat / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D013552:Swine
D000871:Anthelmintics; D001562:Benzimidazoles