Stimulation of protein synthesis by raised extracellular pH in cardiac myocytes and perfused hearts.
Protein synthesis was stimulated in freshly-isolated rats cardiac myocytes by increasing the extracellular pH of Hepes-buffered Tyrode's solutions over the range pH 7.4-8.4. The maximal stimulation was about 45%. Protein synthesis in anterogradely-perfused rat hearts was stimulated by 11% by increasing the pH of the bicarbonate-containing perfusion medium from pH 7.4 to 7.8. This manoeuvre increased intracellular pH by 0.12 units. A concomitant increase in phosphocreatine concentration was observed. These findings are consistent with the hypothesis that intracellular pH may exert profound effects on tissue protein synthesis rates.
Gaitanaki
C J
CJ
Department of Cardiac Medicine, University of London, England.
Sugden
P H
PH
Fuller
S J
SJ
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D001639:Bicarbonates / Q000494:pharmacology; D002021:Buffers; D006863:Hydrogen-Ion Concentration; D009200:Myocardial Contraction / Q000502:physiology; D009206:Myocardium / Q000378:metabolism*; D010477:Perfusion; D010725:Phosphocreatine / Q000378:metabolism; D014176:Protein Biosynthesis; D051381:Rats
D000227:Adenine Nucleotides; D001639:Bicarbonates; D002021:Buffers; D010725:Phosphocreatine