Ultrastructural and cytochemical study of elastic fibers in the ventral aorta of a teleost, Anguilla japonica.
Previous studies have revealed that amorphous elastin and microfibrils are structural entities of mammalian elastic fibers. Elastin shows a wide phylogenetic distribution, but the presence of elastin-associated microfibrils has not been demonstrated in teleost aorta. Thus, we have ultrastructurally and cytochemically examined elastic fibers in the ventral aorta of eel, a teleost, by utilizing routine uranyl acetate and lead double staining, the tannic acid (pH 7.0)-uranyl acetate (TA-UA) method, elastase en bloc digestion, Thiéry's periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) method, and the horseradish-peroxidase-labeled concanavalin A (Con A) method. In the ventral aorta of eel, a little ultrastructural difference between elastic fibers in the intima and media and those in the adventitia was noticed, but in either tunic each elastic fiber was basically composed of a "fibrillar core" and surrounding microfibrils. The fibrillar core was a collection of fibrils which showed a tendency to coalesce with each other, and these constituent fibrils were TA-UA positive and elastase-sensitive, representing their nature of elastin. By contrast, microfibrils associated with the fibrillar core were TA-UA negative and elastase-resistant, and their glycoproteinaceous nature was demonstrated by PA-TCH-SP and Con A methods. Thus, this study provides evidence for the presence of elastin-associated microfibrils in teleost aorta. These results are discussed in relation to the topographical difference of elastic fibers in eel aortic wall.
Isokawa
K
K
Department of Anatomy, Nihon University School of Dentistry, Tokyo, Japan.
Takagi
M
M
Toda
Y
Y
D000811:Anguilla / Q000033:anatomy & histology*; D000818:Animals; D001011:Aorta / Q000032:analysis* / Q000033:anatomy & histology* / Q000648:ultrastructure; D004547:Elastic Tissue / Q000032:analysis / Q000033:anatomy & histology* / Q000648:ultrastructure; D006651:Histocytochemistry; D008854:Microscopy, Electron