Production of baboon (Papio hamadryas) monoclonal antibodies by herpesvirus papio immortalized baboon lymph node cells.
Baboon monoclonal antibodies specific for monomorphic determinants of baboon apolipoprotein B were produced from in vivo primed lymph node cells immortalized by herpesvirus papio (HVP). Two cell lines from the immortalized cells were readily stabilized and grew rapidly. Since Epstein-Barr virus (EBV) cannot be used to immortalize baboon or macaque cells, HVP expands the potential sources of monoclonal antibodies to include baboons, and is the immortalizing agent of choice for macaques as well.
Sharp
R M
RM
Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, TX 78284-2500.
Durocher
C L
CL
Parmenter
J L
JL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000145:classification / Q000276:immunology; D001055:Apolipoproteins B / Q000276:immunology*; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D004797:Enzyme-Linked Immunosorbent Assay; D006564:Herpesviridae; D015151:Immunoblotting; D008077:Lipoproteins, LDL / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology; D008214:Lymphocytes / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D010215:Papio
D000911:Antibodies, Monoclonal; D001055:Apolipoproteins B; D008077:Lipoproteins, LDL