The determination of drugs of abuse in whole blood by means of FPIA and EMIT-dau immunoassays--a comparative study.
Six groups of common drugs of abuse (cannabinoids, benzoylecgonine, opiates, barbiturates, benzodiazepines and amphetamines) were determined in whole blood after acetone precipitation, using enzyme multiplied immunoassay (EMIT dau) and fluorescence polarisation immunoassay (FPIA--Abbott TDx and ADx) methods. Both methods, designed primarily for urine, allowed the determination of all above mentioned class of drugs but amphetamine. Only 1 ml of a pre- or postmortem blood sample was needed. The sensitivity of cannabinoids determination was higher by FPIA. The FPIA method gave more precise results, particularly in the case of autopsy blood. The method was applied for drug screening in autopsy and police blood samples. The results (both positive and negative) were in agreement with those obtained with chromatographic methods.
Bogusz
M
M
Institute of Forensic Medicine, Karl Ruprecht University, Heidelberg, F.R.G.
Aderjan
R
R
Schmitt
G
G
Nadler
E
E
Neureither
B
B
D000662:Amphetamines / Q000097:blood; D001463:Barbiturates / Q000097:blood; D001569:Benzodiazepines / Q000097:blood; D002186:Cannabinoids / Q000097:blood; D003042:Cocaine / Q000031:analogs & derivatives / Q000097:blood; D016231:Fluorescence Polarization Immunoassay; D006801:Humans; D013287:Illicit Drugs / Q000032:analysis*; D007124:Immunoenzyme Techniques; D009294:Narcotics / Q000097:blood; D011237:Predictive Value of Tests
D000662:Amphetamines; D001463:Barbiturates; D002186:Cannabinoids; D013287:Illicit Drugs; D009294:Narcotics; D001569:Benzodiazepines; C005618:benzoylecgonine; D003042:Cocaine