title: [Effect of ATP on glucose-6-phosphate dehydrogenase during erythroblast maturation in the rabbit].
abstract: The glucose-6-phosphate dehydrogenase (G6PD) activity of erythroblasts, separated at different advancing stages of development, shows a marked decline of activity. A proteolytic mechanism, strictly controlled, is likely responsible of this decay, since a sufficient level of enzyme activity still remains in the circulating erythrocyte. In this report we suggest a model that could explain what triggers the mechanism of proteolytic degradation. HPLC analysis of the nucleotide content of erythroblasts and reticulocytes, showed a marked decline of adenine and pyridine nucleotides and of their catabolic products during the cell development. From thermostability tests, at fixed temperature, we have seen that ATP and NADP only, significantly protected the enzyme activity. In this light, we incubated 10 min at increasing temperatures, with and without ATP or NADP lysates of erythroblasts, separated at different stage of development and of reticulocytes. In the absence of nucleotides, we determined for all fractions a T degree break at 42 degrees C. In the presence of NADP all fractions were stabilized with no break point in the range 37-50 degrees C. On the contrary, the presence of ATP caused a progressive shift of the T degrees C break from the most immature erythroblasts (T degree break at 46 degrees C) to the reticulocytes (T degree break at 42 degrees C). Since ATP did not show any protective effect on the reticulocyte enzyme, we hypothesize the presence in these cells of a structurally modified G6PD. Furthermore, these data support our belief that the marked decline of ATP during cellular development, may represent the element responsible for the enzyme modification.
authors.affiliation: Istituto di Chimica Biologica dell'Università degli Studi di Urbino.
authors.forename: S
authors.initials: S
authors.lastname: Rapa
authors.forename: L
authors.initials: L
authors.lastname: Baronciani
authors.forename: I
authors.initials: I
authors.lastname: Orsenigo
authors.forename: G
authors.initials: G
authors.lastname: Piacentini
authors.forename: P
authors.initials: P
authors.lastname: Ninfali
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000743:Anemia, Hemolytic / Q000139:chemically induced / Q000201:enzymology*; D000818:Animals; D004900:Erythroblasts / Q000187:drug effects* / Q000201:enzymology; D005954:Glucosephosphate Dehydrogenase / Q000097:blood*; D009249:NADP / Q000494:pharmacology; D009711:Nucleotides / Q000494:pharmacology; D010659:Phenylhydrazines; D011817:Rabbits; D012156:Reticulocytes / Q000737:chemistry
chemical_list: D009711:Nucleotides; D010659:Phenylhydrazines; C030299:phenylhydrazine; D009249:NADP; D000255:Adenosine Triphosphate; D005954:Glucosephosphate Dehydrogenase
vernacular_title: Effetto dell'ATP sulla glucoso 6 fosfato deidrogenasi durante la maturazione degli eritroblasti di coniglio.