Correlation of the hypo-osmotic swelling test, semen score and the zona-free hamster egg penetration assay in humans.
The correlation between the hypo-osmotic swelling test, the modified Eliasson score for human semen analysis and the hamster egg penetration assay was examined. The results showed a weak but significant correlation between the group of subjects with swelling rates below 50% and the above 80% group. These groups showed hamster egg penetration assay rates of 9 +/- 14% and 39 +/- 29%, respectively (P less than 0.035). A significant correlation was also found between modified Eliasson scores (17 +/- 10 and 5 +/- 7, respectively) and swelling rates below 50% and above 70% (P less than 0.001). These results suggest that spermatozoa showing a low swelling rate have a poor fertilizing capacity. Therefore, the swelling tests and analysis of the patients semen can be used to discriminate sperm quality.
Okada
A
A
2nd Department of Anatomy, School of Medicine, Toho University, Tokyo, Japan.
Inomata
K
K
Matsuhashi
M
M
Fujio
K
K
Miura
K
K
Shiina
K
K
Shirai
M
M
D000177:Acrosome; D000818:Animals; D002118:Calcium / Q000378:metabolism; D006224:Cricetinae; D005260:Female; D005298:Fertility / Q000502:physiology*; D006801:Humans; D007248:Infertility, Male / Q000503:physiopathology; D008297:Male; D009997:Osmotic Pressure; D012661:Semen / Q000502:physiology*; D013075:Sperm Capacitation; D013076:Sperm Count; D013081:Sperm Motility; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000378:metabolism / Q000502:physiology*; D015044:Zona Pellucida
D002118:Calcium