Distribution and characterization of VIP-related peptides in the rat spinal cord.
The possible existence in the rat spinal cord of a peptide related to VIP, VIP(22-28), has been evaluated. VIP contains paired basic aminoacid residues at which posttranslational cleavage of these peptides might occur. The lumbo-sacral region of rat spinal cord had the most VIP(22-28)-like immunoreactivity (ir-VIP(22-28]. Chromatographic analysis of spinal extracts showed that ir-VIP(22-28) consisted of two major peaks, one eluting as authentic VIP(1-28) and the other as VIP(22-28). HPLC confirmed these results, revealing the presence of intact VIP(1-28) and two or more less hydrophobic peptides, one of which corresponded to authentic VIP(22-28). The other two components found have not yet been identified. Further studies are necessary to provide information on the biological significance of VIP(22-28).
Romualdi
P
P
Department of Pharmacology, University of Bologna, Italy.
Lesa
G
G
Cox
B M
BM
Ferri
S
S
D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D008297:Male; D009928:Organ Specificity; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000737:chemistry*; D014660:Vasoactive Intestinal Peptide / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification
D014660:Vasoactive Intestinal Peptide