[Effect of sodium ferulate on arachidonic acid metabolism].
Sodium ferulate (SF) is one of the antiplatelet ingredients in Radix Angleica sinensis. The effect of SF on 14C-arachidonic acid metabolism in washed intact rabbit platelets was studied with radiochromatography and radioautography. SF (0.1-3.2 mmol/L) inhibited the generation of platelet thromboxane B2 in a dose-dependent manner (reduced by 16.7-93.8%) and the IC50 was shown to be 0.762 mmol/L. Simultaneously with the reduction of TXB2 generation, the formation of PGE2 and PGF2 alpha was also reduced significantly after treatment with SF. Using radioimmunoassay SF (0.145-2.32 mmol/L) was found to inhibit rabbit platelet TXB2 formation in a dose-dependent manner. SF (0.58-2.32 mmol/L) also suppressed aortic tissue 6-keto-PGF1 alpha generation in rabbits. At the same concentrations the inhibitory effect of SF on platelet TXB2 formation was greater than that on aortic tissue 6-keto-PGF1 alpha generation. These results indicate that the cyclo-oxygenase activity may be inhibited by SF.
Xu
L N
LN
Department of Pharmacology, Chinese Academy of Medical Sciences, Beijing.
Yu
W G
WG
Tian
J Y
JY
Liu
Q Y
QY
D015121:6-Ketoprostaglandin F1 alpha / Q000097:blood; D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D001011:Aorta / Q000378:metabolism; D001095:Arachidonic Acids / Q000378:metabolism*; D001792:Blood Platelets / Q000187:drug effects; D003373:Coumaric Acids / Q000494:pharmacology*; D015237:Dinoprost / Q000097:blood; D015232:Dinoprostone / Q000097:blood; D008297:Male; D011817:Rabbits; D051381:Rats; D013929:Thromboxane B2 / Q000097:blood
D000925:Anticoagulants; D001095:Arachidonic Acids; D003373:Coumaric Acids; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; C004999:ferulic acid; D015237:Dinoprost; D015232:Dinoprostone