Antibody to a soluble protein purified from brain selectively labels layer V corticofugal projection neurons in rat neocortex.
An antibody to a soluble protein (protein 36) isolated and purified from rat brain labels the cell bodies and processes of pyramidal cells within layer V of the rat neocortex. We have used the fluorescent retrograde axonal tracer, Fast blue, in combination with FITC immunocytochemistry to determine the projection sites of the cortical neurons detected by this antibody. Retrogradely labeled pyramidal tract neurons and corticotectal neurons are labeled with the protein 36 antibody, but the callosally projecting neurons within layer V are not. Thus within the neocortex the antibody to protein 36 may selectively detect a particular class of neuron, the corticofugal projection neurons of layer V.
Stanfield
B B
BB
Laboratory of Clinical Science, National Institute of Mental Health, National Institutes of Health Animal Center, Poolesville, MD 20837.
Jacobowitz
D M
DM
D000818:Animals; D000906:Antibodies; D001921:Brain / Q000033:anatomy & histology*; D002540:Cerebral Cortex / Q000033:anatomy & histology*; D005455:Fluorescent Antibody Technique; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009474:Neurons / Q000166:cytology*; D010296:Parietal Lobe / Q000033:anatomy & histology; D051381:Rats; D011919:Rats, Inbred Strains; D013702:Temporal Lobe / Q000033:anatomy & histology
D000906:Antibodies; D009419:Nerve Tissue Proteins