title: Monoclonal antibody to 2-amino-3-methylimidazo(4,5-f)quinoline, a dietary carcinogen.
abstract: In order to investigate relationships between human carcinogenesis and dietary carcinogens, one hybridoma cell line secreting a monoclonal antibody against 2-amino-3-methylimidazo(4,5-f)quinoline (IQ), a dietary carcinogen, was produced by fusing splenocytes from Balb/c mice immunized with IQ-Lysine(Lys)-Ascaris protein conjugate. The subclass of monoclonal anti-IQ antibody was determined by double immunodiffusion using culture medium and identified as IgG1. Monoclonal anti-IQ antibody was purified from ascites fluids of Balb/c mice with affinity chromatography on Protein A-Sepharose CL4B and analyzed concerning its cross-reactivity and sensitivity with RIA. Finally, we showed that our monoclonal antibody recognized IQ, 2-amino-3,4-dimethylimidazo(4,5-f)quinoline (MeIQ) and several beta-carbolines more intensely and that the sensitivity to IQ was 23 nmol in 50% displacement.
authors.affiliation: Department of Hygiene & Preventive Medicine, Faculty of Medicine, University of Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yanagisawa
authors.forename: T
authors.initials: T
authors.lastname: Tachikawa
authors.forename: S
authors.initials: S
authors.lastname: Shin
authors.forename: O
authors.initials: O
authors.lastname: Wada
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D001202:Ascitic Fluid / Q000276:immunology; D002243:Carbolines / Q000276:immunology; D002273:Carcinogens; D002846:Chromatography, Affinity; D004032:Diet; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D011804:Quinolines / Q000276:immunology*; D011863:Radioimmunoassay
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D002243:Carbolines; D002273:Carcinogens; D007074:Immunoglobulin G; D011804:Quinolines; C029216:2-amino-3-methylimidazo(4,5-f)quinoline; C036989:2-amino-3,4-dimethylimidazo(4,5-f)quinoline