title: A comparison of results for cholesterol in human serum obtained by the Reference Method and by the Definitive Method of the National Reference System for cholesterol.
abstract: Here we compare the Reference Method (I) and the Definitive Method (II) for measurement of cholesterol in serum. For pure cholesterol solutions, values by I agree with values by II, but for fresh, frozen, or lyophilized sera, values by I average 1.6% higher than values by II. We conclude that an undetected interference is associated with I. However, the observed difference does not diminish the usefulness of I as an accuracy base for cholesterol measurements, because it is clinically insignificant at present.
authors.affiliation: Organic Analytical Research Division, National Institute of Standards and Technology, Gaithersburg, MD 20899.
authors.forename: P
authors.initials: P
authors.lastname: Ellerbe
authors.forename: G L
authors.initials: GL
authors.lastname: Myers
authors.forename: G R
authors.initials: GR
authors.lastname: Cooper
authors.forename: H S
authors.initials: HS
authors.lastname: Hertz
authors.forename: L T
authors.initials: LT
authors.lastname: Sniegoski
authors.forename: M J
authors.initials: MJ
authors.lastname: Welch
authors.forename: E
authors.initials: E
authors.lastname: White
mesh_terms: D001793:Blood Preservation / Q000379:methods; D002624:Chemistry, Clinical / Q000379:methods / Q000592:standards*; D002784:Cholesterol / Q000097:blood* / Q000592:standards; D005188:False Negative Reactions; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006868:Hydrolysis; D007753:Laboratories / Q000592:standards; D012015:Reference Standards; D015203:Reproducibility of Results; D014481:United States
chemical_list: D002784:Cholesterol