[The effect of hormones and growth factors on the proliferation of cultured mouse granulosa cells].
A method of obtaining granulosa cell culture reacting to the action of gonadotropins and growth factors is described. The efficiency of cell cloning is enhanced under influence of insulin, epidermal growth factor (EGF) and fibroblast growth factors (FGF). Stimulation of proliferation by the latter two factors is seen in the medium with the low serum concentration. Luteinizing and follicle-stimulating inhibit the cell growth in culture. The role of growth factors and gonadotropins in regulation of granulosa proliferation in mammalian ovarian follicles is discussed.
Vorob'eva
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D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D006062:Gonadotropins / Q000494:pharmacology*; D006107:Granulosa Cells / Q000166:cytology / Q000187:drug effects*; D006133:Growth Substances / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA
D006062:Gonadotropins; D006133:Growth Substances; D007328:Insulin
Vliianie gormonov i faktorov rosta na proliferatsiiu kul'tiviruemykh kletok granulezy mysheĭ.