Intra-epidermal retention of type VII collagen in a patient with recessive dystrophic epidermolysis bullosa.
An infant born with severe blisters on the limbs, face, trunk, and oral mucosa was diagnosed by light and electron microscopy to have recessive dystrophic epidermolysis bullosa. Transmission electron microscopy showed that the basal lamina remained with the epidermis and that the floor of the blister was exposed collagen of the papillary dermis. No banded anchoring fibrils were observed along either the roof or the floor of the blister; however, small filamentous structures, possibly immature anchoring fibrils, extended down from the lamina densa along the blister roof. Some basal and suprabasal keratinocytes contained large vesicles filled with filamentous matrix of variable electron density. Immunofluorescent staining of skin for type VII collagen showed sparse and irregular staining of type VII collagen along the blister roof, and intense intracellular labeling for type VII collagen in clusters of epidermal cells in basal and suprabasal layers. Type VII collagen appeared to be synthesized by keratinocytes but not secreted.
Smith
L T
LT
Department of Biological Structure, University of Washington, Seattle 98195.
Sybert
V P
VP
D000911:Antibodies, Monoclonal; D003094:Collagen / Q000378:metabolism*; D004820:Epidermolysis Bullosa / Q000209:etiology / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D008854:Microscopy, Electron; D010375:Pedigree; D012867:Skin / Q000032:analysis
D000911:Antibodies, Monoclonal; D003094:Collagen