title: Progress-curve equations for reversible enzyme-catalysed reactions inhibited by tight-binding inhibitors.
abstract: The rate equation for a tight-binding inhibitor of an enzyme-catalysed first-order reversible reaction was used to derive two integrated equations. One of them covers the situations in which competitive, uncompetitive or non-competitive inhibition occurs and the other refers to the special non-competitive case where the two inhibition constants are equal. For these equations, graphical and non-linear regression methods are proposed for distinguishing between types of inhibition and for calculating inhibition constants from progress-curve data. The application of the non-linear regression to the analysis of stimulated progress curves in the presence of a tight-binding inhibitor is also presented. The results obtained are valid for any type of 'dead-end'-complex-forming inhibitor and can be used to characterize an unknown inhibitor on the basis of progress curves.
authors.affiliation: Department of Biochemistry, Institute of Biological Sciences, Bucharest, Romania.
authors.forename: S E
authors.initials: SE
authors.lastname: Szedlacsek
authors.forename: V
authors.initials: V
authors.lastname: Ostafe
authors.forename: R G
authors.initials: RG
authors.lastname: Duggleby
authors.forename: M
authors.initials: M
authors.lastname: Serban
authors.forename: M O
authors.initials: MO
authors.lastname: Vlad
mesh_terms: D001667:Binding, Competitive; D002384:Catalysis; D004798:Enzymes; D007700:Kinetics; D008433:Mathematics
chemical_list: D004798:Enzymes