title: Rapid labeling of lipoproteins in plasma with radioactive cholesterol. Application for measurement of plasma cholesterol esterification.
abstract: In order to efficiently and rapidly label lipoproteins in plasma with [3H]cholesterol, micelles consisting of lysophosphatidylcholine (lysoPC) and [3H]cholesterol (molar ratio, 50:1) were prepared. When trace amounts of these micelles were injected into plasma, [3H]cholesterol rapidly equilibrated among the plasma lipoproteins, as compared to [3H]cholesterol from an albumin-stabilized emulsion. The distributions of both [3H]cholesterol and unlabeled free cholesterol in plasma lipoproteins were similar in labeled plasma samples. This method of labeling can be used for the measurement of cholesterol esterification, or lecithin:cholesterol acyltransferase activity, in small amounts (20-40 microliters) of plasma samples.
authors.affiliation: Burnsides Research Laboratory, Department of Food Science, University of Illinois, Urbana 61801.
authors.forename: F T
authors.initials: FT
authors.lastname: Yen
authors.forename: T
authors.initials: T
authors.lastname: Nishida
mesh_terms: D002784:Cholesterol / Q000097:blood*; D002788:Cholesterol Esters / Q000097:blood*; D004951:Esterification; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D008244:Lysophosphatidylcholines; D008823:Micelles; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000097:blood; D014316:Tritium
chemical_list: D002788:Cholesterol Esters; D008074:Lipoproteins; D008244:Lysophosphatidylcholines; D008823:Micelles; D014316:Tritium; D002784:Cholesterol; D007862:Phosphatidylcholine-Sterol O-Acyltransferase