title: Anti-PreS2 antibody assay for evaluating immune responses among recipients of recombinant hepatitis B PreS2 + S vaccine.
abstract: A competitive radioimmunoassay was developed for measuring hepatitis B virus (HBV) anti-PreS2 antibody. The assay has been demonstrated to be highly specific for anti-PreS2 antibody and not subject to interference by other antibodies to HBV-specific antigens. This assay was used to evaluate anti-PreS2 antibody responses in a hepatitis B PreS2 + S vaccine human clinical trial in healthy adults.
authors.affiliation: Virus and Cell Biology Research, Merck Sharp & Dohme Research Laboratories, West Point, Pennsylvania.
authors.forename: W M
authors.initials: WM
authors.lastname: Hurni
authors.forename: R R
authors.initials: RR
authors.lastname: Roehm
authors.forename: W J
authors.initials: WJ
authors.lastname: Miller
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000032:analysis*; D005069:Evaluation Studies as Topic; D006508:Hepatitis Antibodies / Q000032:analysis; D006509:Hepatitis B / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006515:Hepatitis B virus / Q000276:immunology*; D006801:Humans; D011498:Protein Precursors / Q000276:immunology*; D011863:Radioimmunoassay / Q000379:methods*; D014612:Vaccines / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D006508:Hepatitis Antibodies; D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; D014612:Vaccines; D014614:Vaccines, Synthetic; C050807:presurface protein 2, hepatitis B surface antigen