title: Isolation of several fractions from human serum, inhibiting rabbit cartilage metabolism in vitro.
abstract: In contrast to the well characterized serum stimulators of cartilage metabolism, information is scarce on the nature of circulating inhibitors. Human serum was fractionated by molecular sieving chromatography on Sephadex G-200, G-75, G-50 and Biogel P-4 gels. Six fractions with molecular weights of 150, 45, 30, 16, 9 and 1.2 kD inhibited [35S]sulphate incorporation into rabbit cartilage segments. All fractions but the smallest one exhibited their inhibitory effect only in the serum-stimulated cartilage. The 1.2 kD fraction impaired [35S]sulphate and [3H]methyl-thymidine incorporation into the cartilage segments in both stimulated and basal conditions. A seventh inhibitory fraction corresponded to the serum salt peak.
authors.affiliation: Department of Pediatrics, University of Antwerp, Belgium.
authors.forename: M V
authors.initials: MV
authors.lastname: Du Caju
authors.forename: R P
authors.initials: RP
authors.lastname: Rooman
mesh_terms: D000818:Animals; D001665:Binding Sites; D001798:Blood Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D002356:Cartilage / Q000187:drug effects* / Q000378:metabolism; D002850:Chromatography, Gel; D011817:Rabbits; D013431:Sulfates / Q000378:metabolism; D013936:Thymidine / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D001798:Blood Proteins; D013431:Sulfates; C012439:methylthymidine; D013936:Thymidine