The gene for the beta-subunit of retinal transducin (Gnb-1) maps to distal mouse chromosome 4, and related sequences map to mouse chromosomes 5 and 8.
The heterotrimeric G protein transducin releases cGMP-phosphodiesterase from inhibition in retinal rod photoreceptor cells when stimulated by light-activated rhodopsin. As a result the level of cGMP goes down, the rod plasma membrane hyperpolarizes, and the release of neurotransmitter is modified. We have used a bovine cDNA for the beta-subunit of transducin (G beta 1) to map its gene Gnb-1 to distal mouse chromosome 4. This cDNA also identified two other homologous sequences in the mouse genome. One of the sequences was on chromosome 5 which we identified as the locus of Gnb-2, a second G protein beta-subunit gene. The other sequence was on chromosome 8 and is either a pseudogene or an as yet undiscovered third G beta-subunit gene, here termed Gnb-3.
Danciger
M
M
Jules Stein Eye Institute, UCLA School of Medicine 90024-1771.
Farber
D B
DB
Peyser
M
M
Kozak
C A
CA
D000818:Animals; D000830:Animals, Laboratory / Q000235:genetics; D000835:Animals, Wild / Q000235:genetics; D002874:Chromosome Mapping; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics; D005796:Genes; D006801:Humans; D006822:Hybrid Cells / Q000032:analysis; D051379:Mice / Q000235:genetics*; D013045:Species Specificity; D015293:Transducin / Q000235:genetics*
D004247:DNA; D015293:Transducin