Cell type dependent activation of poly (ADP-ribose) synthesis following treatment with etoposide.
Treatment of human non-lymphoid cell lines, HL-60 and U937, with etoposide stimulated poly (ADP-ribose) synthesis three- to fourfold, whereas no significant effects were observed in the lymphoid cell lines, Molt4 and CEM. This was confirmed by either an increased uptake of radio-labelled NAD into the acid-insoluble fraction or a fall in cellular NAD levels, which was counteracted by 3-aminobenzamide, an inhibitor of poly(ADP-ribose) polymerase. On the other hand, another DNA damaging agent, N-methyl-N'-nitro-N-nitrosoguanidine augmented poly(ADP-ribose) synthesis equally in both cell types. These results taken together indicate that the activation of poly(ADP-ribose) synthesis following exposure to etoposide is a cell type specific phenomenon.
Kubota
M
M
Department of Pediatrics, Kyoto University, Japan.
Tanizawa
A
A
Hashimoto
H
H
Shimizu
T
T
Takimoto
T
T
Kitoh
T
T
Akiyama
Y
Y
Mikawa
H
H
D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000187:drug effects; D004249:DNA Damage; D005047:Etoposide / Q000494:pharmacology*; D006801:Humans; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009702:Nucleoside Diphosphate Sugars / Q000096:biosynthesis*; D011064:Poly Adenosine Diphosphate Ribose / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
D009702:Nucleoside Diphosphate Sugars; D008769:Methylnitronitrosoguanidine; D011064:Poly Adenosine Diphosphate Ribose; D005047:Etoposide; D004247:DNA