[Immunohistochemical studies of ulcerative colitis--expression of HLA-DR antigen by colonic epithelium and the role of dendritic cells in the lamina propria].
In 27 patients with ulcerative colitis the colonic mucosa was analyzed by an immunohistochemical technique. A quantitative evaluation for lymphocyte subsets shows significantly-increased numbers of CD3+ cells, CD4+ cells, CD8+ cells and Leu7+ cells in ulcerative colitis cases of histological grades 4 and 5 by Matts's classification comparing to controls. However, CD4/CD8 ratio in each histological grade of ulcerative colitis was similar to that in normal controls. The HLA-DR antigen on the colonic epithelium was frequently expressed in patients with grades 3 to 5 (84.2%), and in active patients (88.9%). The antigens were also expressed on the lamina propria mononuclear cells, which coincided with the distribution of S-100+ dendritic cells. Moreover double staining for S-100 antigen and IL-2R antigen revealed that most of the S-100+ dendritic cells were also IL-2R positive. These findings seem to provide evidence for the important role of lamina propria dendritic cells in the immunological pathogenesis of ulcerative colitis.
Kimura
M
M
First Department of Internal Medicine, Nagoya University School of Medicine.
Morise
K
K
D003093:Colitis, Ulcerative / Q000276:immunology* / Q000473:pathology; D003106:Colon / Q000276:immunology* / Q000473:pathology; D003713:Dendritic Cells / Q000502:physiology*; D004848:Epithelium / Q000276:immunology; D006684:HLA-DR Antigens / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000166:cytology
D006684:HLA-DR Antigens