Autoxidation of ascorbic acid catalyzed by a semisynthetic enzyme.
The semisynthetic enzyme 6 was prepared by alkylation of the cysteine-25 sulfhydryl group of papain with the bipyridine 5 and was shown to stoichiometrically bind copper ion; 7 catalyzed the autoxidation of ascorbic acid derivatives with saturation kinetics approximately 20-fold faster than a model system using 3-Cu(II).
Germanas
J P
JP
Laboratory of Bioorganic Chemistry and Biochemistry, Rockefeller University, New York, New York 10021.
Kaiser
E T
ET
D001205:Ascorbic Acid / Q000378:metabolism*; D007700:Kinetics; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000138:chemical synthesis / Q000378:metabolism*
D010088:Oxidoreductases; D001205:Ascorbic Acid