title: A low-protein diet protects uremic rats against the negative sequelae of metabolic acidosis.
abstract: Metabolic acidosis is a common finding in uremia. The metabolic consequences, however, are poorly understood. Thus, the aim of our study was to assess the effect of chronic metabolic acidosis in 5/6-nephrectomized male Sprague-Dawley rats given a normal (18%; n = 19) and a low-protein diet (8%; n = 23). Each of these groups was sequentially given CaCO3 and CaCl2 in the drinking water for a fortnight each. The animals were randomly assigned to start either with CaCO3 or CaCl2 (random cross-over design). The blood pH decreased significantly in both CaCl2 groups (18% protein: CaCO3 7.18 vs. CaCl2 7.11; 8% protein: CaCO3 7.26 vs. CaCl2 7.09) as did standardized base excess (18% protein: CaCO3-5.9 vs. CaCl2-9.7; 8% protein: CaCO3-3.6 vs. CaCl2-12.6). Food intake declined during acidosis in both groups, but more in the 18% protein group. The same occurred with body weight (g) in the 18% group, which decreased dramatically (8% protein: CaCO3 389 vs. CaCl2 390; 18% protein: CaCO3 413 vs. CaCl2 366). The change in body weight was reflected in the urinary urea excretion (mg/24 h/g food) (8% protein: CaCO3 0.9 vs. CaCl2 1.0; 18% protein: CaCO3 2.2 vs. CaCl2 30.8). There was a significant increase in proteinuria (mg/24 h) in the 8% group (CaCO3 10 vs. CaCl2 15), while in the 18% group no real change occurred (CaCO3 24 vs. CaCl2 18). Factoring the proteinuria for food intake, however, also resulted in a tendency towards an increased proteinuria in the 18% group.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Clinic of Nephrology, Klinikum Mannheim, University of Heidelberg, FRG.
authors.forename: N
authors.initials: N
authors.lastname: Gretz
authors.forename: J J
authors.initials: JJ
authors.lastname: Lasserre
authors.forename: K
authors.initials: K
authors.lastname: Langer
authors.forename: M
authors.initials: M
authors.lastname: Strauch
mesh_terms: D000141:Acidosis, Renal Tubular / Q000378:metabolism / Q000652:urine*; D000818:Animals; D000855:Anorexia / Q000378:metabolism; D004044:Dietary Proteins / Q000008:administration & dosage*; D008297:Male; D011507:Proteinuria / Q000652:urine; D051381:Rats; D011919:Rats, Inbred Strains; D014511:Uremia / Q000378:metabolism*
chemical_list: D004044:Dietary Proteins