[Differentiation of human sperm cells using flow cytometry].
An improved method for differential staining and high-resolution flow cytometric measurement of human sperm cells is presented. Involving a mild pretreatment with citric acid/detergent and staining with DAPI, the new procedure provides excellent preservation and good discrimination of all cells present in normal and pathologic semen samples.
Otto
F J
FJ
Abteilung Experimentelle Tumorforschung, Fachklinik Hornheide, Münster.
Hettwer
H
H
Hofmann
N
N
D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000473:pathology*; D013076:Sperm Count; D013081:Sperm Motility / Q000502:physiology; D013091:Spermatogenesis / Q000502:physiology; D013094:Spermatozoa / Q000473:pathology*
Differenzierung menschlicher Spermazellen mit Hilfe der Durchflusszytometrie.