A new sensitive microassay for the measurement of erythrocyte glycogen.
Conventional ethanol precipitation of sub-microgram amounts of glycogen leads to low yields (less than 50%). Quantitative recoveries of 90% were attained, however, when the isolation temperature was raised to 50 degrees C and ethanol was replaced by the less polar propan-2-ol. This improvement enabled development of an erythrocyte assay for glycogen which was both sensitive (0.1 microgram glycogen) and required only 1 ml of whole blood. 26 paediatric specimens were analysed and a reference range of values from undetected to 78 micrograms glycogen/g haemoglobin (Hb) was obtained.
Farquharson
J
J
Department of Biochemistry, Royal Hospital for Sick Children, Glasgow, Scotland, UK.
Jamieson
E C
EC
MacPhee
G B
GB
Logan
R W
RW
D000433:1-Propanol; D004912:Erythrocytes / Q000032:analysis*; D006003:Glycogen / Q000097:blood*; D006358:Hot Temperature; D006801:Humans; D008832:Microchemistry
D006003:Glycogen; D000433:1-Propanol