title: Effects of alfalfa nod gene-inducing flavonoids on nodABC transcription in Rhizobium meliloti strains containing different nodD genes.
abstract: Transcription of the nodulation genes nodABC in Rhizobium meliloti requires a plant flavonoid signal and nodD, a family of bacterial regulatory genes (nodD1, nodD2, and nodD3). Results from this study show that all previously identified nod gene inducers released by alfalfa seeds and roots induced nodABC-lacZ transcription in R. meliloti containing extra copies of nodD1, but only 4,4'-dihydroxy-2'-methoxychalcone gave high levels of induction with extra copies of nodD2. While mixtures of the methoxychalcone and luteolin showed a positive synergism with extra NodD1 protein, they apparently competed for binding to the NodD2 protein.
authors.affiliation: Department of Agronomy and Range Science, University of California, Davis 95616.
authors.forename: U A
authors.initials: UA
authors.lastname: Hartwig
authors.forename: C A
authors.initials: CA
authors.lastname: Maxwell
authors.forename: C M
authors.initials: CM
authors.lastname: Joseph
authors.forename: D A
authors.initials: DA
authors.lastname: Phillips
mesh_terms: D000217:Acyltransferases; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D005419:Flavonoids / Q000378:metabolism* / Q000494:pharmacology; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005809:Genes, Regulator; D005838:Genotype; D047311:Luteolin; D000455:Medicago sativa / Q000502:physiology*; D017351:N-Acetylglucosaminyltransferases; D012231:Rhizobium / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D005419:Flavonoids; D000217:Acyltransferases; C060770:NodA protein, Rhizobiales; D017351:N-Acetylglucosaminyltransferases; C060771:NodC protein, Rhizobiales; D047311:Luteolin