title: Birth of normal calves resulting from bovine oocytes matured, fertilized, and cultured with cumulus cells in vitro up to the blastocyst stage.
abstract: Bovine oocytes matured in vitro were fertilized in high proportions (92% of matured oocytes) by sperm capacitated with Ca ionophore A23187. Eight percent of inseminated oocytes that were denuded 96 h after insemination developed to the morula stage when cultured for 6-120 h after insemination with cumulus cells from the original oocytes. Inseminated oocytes denuded 96 h after insemination developed to the blastocyst stage when cultured with or without cumulus cells or in the conditioned medium from 96 h to 168-216 h after insemination (9.0%, 8.1%, and 6.8% of inseminated oocytes respectively). Six frozen-thawed blastocysts were transferred nonsurgically to 3 recipients (2 embryos/recipient). Two of the 3 recipients became pregnant, with one delivering live twins at term. Seven fresh blastocysts were transferred nonsurgically to 6 recipients (1-2 embryos/recipient). Three of the 6 recipients became pregnant, with 2 delivering live calves.
authors.affiliation: School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Fukuda
authors.forename: M
authors.initials: M
authors.lastname: Ichikawa
authors.forename: K
authors.initials: K
authors.lastname: Naito
authors.forename: Y
authors.initials: Y
authors.lastname: Toyoda
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D002417:Cattle; D002478:Cells, Cultured; D004624:Embryo Transfer / Q000662:veterinary*; D005260:Female; D005307:Fertilization in Vitro; D009865:Oocytes / Q000254:growth & development*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000502:physiology*