title: Analysis of bumetanide in human urine by high-performance liquid chromatography with fluorescence detection and gas chromatography/mass spectrometry.
abstract: Bumetanide is a potent diuretic. Its concentration in urine after therapeutic doses is in the low ng/mL range. A procedure using reversed-phase HPLC with fluorescence detection is described. Using this procedure, the authors have been able to measure bumetanide in a urine sample at 10 ng/mL with a signal-to-noise ratio of 7. Therefore, the detection limit is lower than this concentration. Confirmation is by a GC/MS procedure after methylating the diuretic. Derivatization is by extractive alkylation with tetrabutylammonium hydrogen sulfate as the phase transfer reagent and iodomethane as the methylating agent.
authors.affiliation: Department of Laboratory Medicine, Foothills Hospital, Calgary, Alberta, Canada.
authors.forename: C Y
authors.initials: CY
authors.lastname: Gradeen
authors.forename: D M
authors.initials: DM
authors.lastname: Billay
authors.forename: S C
authors.initials: SC
authors.lastname: Chan
mesh_terms: D002034:Bumetanide / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D004232:Diuretics / Q000652:urine*; D004300:Doping in Sports; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D013050:Spectrometry, Fluorescence; D015813:Substance Abuse Detection / Q000379:methods*
chemical_list: D004232:Diuretics; D002034:Bumetanide