African swine fever virus: purification of capsomeres released by lipase.
The virus capsomeres of the outer and inner layers of capsids were effectively released simultaneously from purified virions by lipase digestion and were purified by a linear gradient ultracentrifugation. The capsid consisted of an array of double layers of uniformly arranged individual capsomeres where a lipid(s) served as a matrix in between the capsomeres.
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Plum Island Animal Disease Center, USDA, Greenport, New York 11944.
Murakami
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Y
Saiz
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D000358:African Swine Fever Virus / Q000032:analysis* / Q000648:ultrastructure; D002213:Capsid / Q000302:isolation & purification* / Q000648:ultrastructure; D007497:Iridoviridae / Q000032:analysis*; D008049:Lipase; D008854:Microscopy, Electron; D014771:Virion / Q000032:analysis / Q000648:ultrastructure
D008049:Lipase