title: Structural organization of the hCTLA-1 gene encoding human granzyme B.
abstract: Cytotoxic T lymphocytes (CTLs) and natural killer/lymphokine-activated cells produce granzymes, a family of serine esterase proteins located in cytoplasmic granules. These might be involved in different cytotoxic pathways. We report the structural organization of the human gene encoding granzyme B (hCTLA-1). A 4.75-kb genomic DNA fragment containing all the sequences of granzyme B-encoding cDNA clones has been sequenced. The gene is composed of five exons and four introns. A comparison with the genomic organization of murine CCP1/CTLA-1 showed very similar structure and a 76% nucleotide homology in the coding sequences. This suggests that both genes may have a common ancestor. No typical regulatory element was detected in the 1160 bp upstream from the ATG start codon. The detection of a second locus related to hCTLA-1 is also described.
authors.affiliation: INSERM U93, Centre Hayem, Hôpital St. Louis, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Haddad
authors.forename: M V
authors.initials: MV
authors.lastname: Clément
authors.forename: O
authors.initials: O
authors.lastname: Bernard
authors.forename: C J
authors.initials: CJ
authors.lastname: Larsen
authors.forename: L
authors.initials: L
authors.lastname: Degos
authors.forename: M
authors.initials: M
authors.lastname: Sasportes
authors.forename: D
authors.initials: D
authors.lastname: Mathieu-Mahul
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D004247:DNA / Q000235:genetics; D005796:Genes; D053804:Granzymes; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D012697:Serine Endopeptidases / Q000235:genetics*
chemical_list: D004247:DNA; C509213:GZMB protein, human; D053804:Granzymes; C509214:Gzmb protein, mouse; D012697:Serine Endopeptidases