title: Intrauterine versus postnatal repair of created pulmonary artery stenosis in the lamb. Morphologic comparison.
abstract: Experimental lamb models were used for intrauterine creation of pulmonary artery stenosis and later intrauterine repair or postnatal repair. Intrauterine creation of pulmonary artery stenosis was performed in 23 fetal lambs at 90 +/- 1 days of gestation. Eight lambs underwent intrauterine repair of pulmonary artery stenosis at 135 +/- 1 days of gestation and were studied 110 +/- 13 days after repair. Seven lambs underwent postnatal repair at 57 +/- 9 days after birth and were studied 162 +/- 32 days after repair. Eight fetal lambs with unrepaired pulmonary artery stenosis were studied 89 +/- 18 days after birth. All study lambs were compared with normal control lambs. The systolic right ventricular pressure was significantly higher after unrepaired stenosis (78.6 +/- 6.8 mm Hg) than in other lambs, but there was no statistically significant difference after intrauterine repair (23.3 +/- 2.9 mm Hg), postnatal repair (25.9 +/- 3.4 mm Hg), and normal lambs (21.6 +/- 1.1 mm Hg). The systolic pulmonary artery pressure was also not statistically different in these three groups. The weight measurements were age-adjusted for comparison of postnatal and intrauterine repair with normal lambs. The adjusted heart weights were similar in the three groups. The comparison of the adjusted heart weight/adjusted body weight ratio (10(-3) showed a significantly higher ratio in postnatal repair (7.4 +/- 0.1) than in intrauterine repair (6.1 +/- 0.1). The adjusted right ventricular weight/adjusted left ventricular weight ratio was significantly higher in the postnatal repair group (0.71 +/- 0.01) than in both the intrauterine repair group (0.59 +/- 0.01) and normal lambs (0.59 +/- 0.01). The transverse myocyte diameter was not statistically different in all groups of animals and there were no ultrastructural changes even when the pulmonary stenosis was unrepaired. We conclude that intrauterine repair was more satisfactory than postnatal repair in terms of age-adjusted heart weight results, but we did not find any advantages of intrauterine repair in terms of histologic and ultrastructural changes.
authors.affiliation: Department of Experimental Surgery, Faculté de Médecine Necker, Enfants Malades, Paris, France.
authors.forename: O
authors.initials: O
authors.lastname: Bical
authors.forename: P
authors.initials: P
authors.lastname: Gallix
authors.forename: M
authors.initials: M
authors.lastname: Toussaint
authors.forename: P
authors.initials: P
authors.lastname: Landais
authors.forename: D
authors.initials: D
authors.lastname: Gaillard
authors.forename: J
authors.initials: J
authors.lastname: Karam
authors.forename: J Y
authors.initials: JY
authors.lastname: Neveux
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001794:Blood Pressure; D003251:Constriction, Pathologic; D005315:Fetal Diseases / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D005865:Gestational Age; D006321:Heart / Q000503:physiopathology; D009206:Myocardium / Q000473:pathology; D011651:Pulmonary Artery / Q000002:abnormalities / Q000503:physiopathology / Q000601:surgery*; D012756:Sheep