title: Leukocyte surface markers in Rana catesbeiana, identified using mouse monoclonal antibodies.
abstract: Monoclonal antibodies (mAbs), each reactive with thymocytes, neutrophils, and thrombocytes of the bullfrog, Rana catesbeiana, were raised and used for studies of hematopoiesis of metamorphic larvae. The mAbRc-T1, which immunoreacted exclusively with thymocytes in adults, was also strongly reactive to larval thymocytes. Thus the determinant Rc-T1 may provide an appropriate marker for thymocytes, although antigenic sharing is also seen in larval ovarian tissue. Neutrophils detectable by both mAbRc-N1 and Rc-N2 originated primarily in the mesonephros and showed up increasingly in peripheral blood of larvae. Thrombocytes detectable by mAbRc-P may originate in the larval spleen and/or liver but not in the mesonephros.
authors.affiliation: Department of Biology, Faculty of Science, Hirosaki University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sugiyama
authors.forename: A
authors.initials: A
authors.lastname: Amenomori
authors.forename: K
authors.initials: K
authors.lastname: Hatakeyama
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology*; D000943:Antigens, Differentiation / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology*; D006410:Hematopoiesis; D007814:Larva / Q000502:physiology; D007962:Leukocytes / Q000276:immunology*; D008675:Metamorphosis, Biological; D011892:Rana catesbeiana / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D000954:Antigens, Surface