Photodynamic and non-photodynamic action of several porphyrins on the activity of some heme-enzymes.
The action of porphyrins, uroporphyrin I and III (URO I and URO III), pentacarboxylic porphyrin I (PENTA I), coproporphyrin I and III (COPRO I and COPRO III), protoporphyrin IX (PROTO IX) and mesoporphyrin (MESO), on the activity of human erythrocytes delta-aminolevulinic acid dehydratase, porphobilinogenase, deaminase and uroporphyrinogen decarboxylase in the dark and under UV light was investigated. Both photoinactivation and light-independent inactivation was found in all four enzymes using URO I as sensitizer. URO III had a similar action as URO I on porphobilinogenase and deaminase and PROTO IX exerted equal effect as URO I on delta-aminolevulinic acid dehydratase and uroporphyrinogen decarboxylase. Photodynamic efficiency of the porphyrins was dependent on their molecular structure. Selective photodecomposition of enzymes by URO I, greater specificity of tumor uptake by URO I and enhanced porphyrin synthesis by tumors from delta-aminolevulic acid, with predominant formation of URO I, underline the possibility of using URO I in detection of malignant cells and photodynamic therapy.
Afonso
S G
SG
Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), University of Buenos Aires, Argentine.
Chinarro
S
S
Muñoz
J J
JJ
de Salamanca
R E
RE
Batlle
A M
AM
D000642:Ammonia-Lyases / Q000037:antagonists & inhibitors / Q000097:blood* / Q000528:radiation effects; D002262:Carboxy-Lyases / Q000097:blood*; D004912:Erythrocytes / Q000201:enzymology*; D006420:Hemeproteins / Q000037:antagonists & inhibitors / Q000378:metabolism* / Q000528:radiation effects; D006801:Humans; D011163:Hydroxymethylbilane Synthase / Q000037:antagonists & inhibitors / Q000097:blood*; D007700:Kinetics; D010777:Photochemistry; D000623:Porphobilinogen Synthase / Q000037:antagonists & inhibitors / Q000097:blood* / Q000528:radiation effects; D011166:Porphyrins / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014466:Ultraviolet Rays; D014575:Uroporphyrinogen Decarboxylase / Q000037:antagonists & inhibitors / Q000097:blood*
D006420:Hemeproteins; D011166:Porphyrins; D011163:Hydroxymethylbilane Synthase; D002262:Carboxy-Lyases; D014575:Uroporphyrinogen Decarboxylase; D000623:Porphobilinogen Synthase; D000642:Ammonia-Lyases; C100132:porphobilinogenase