N-methylmitomycin A cross-linking to nucleosomal structure.
The cross-linking reaction of N-methylmitomycin A to 175-bp calf thymus nucleosomes and to reconstituted core particles having known DNA sequences was examined using gel-electrophoresis techniques. The nucleosome structure, as the CAP-DNA complex, leads to a decreased covalent binding in comparison to protein-free DNA, suggesting, in addition to sequence specificity, precise geometrical requirements for bifunctional covalent addition of the drug to the nucleic acid.
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Department of Organic Chemistry, University of Padua, Italy.
Palumbo
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Palu
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Crothers
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DM
D000818:Animals; D001482:Base Composition / Q000187:drug effects / Q000502:physiology; D002417:Cattle; D003432:Cross-Linking Reagents; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D004347:Drug Interactions; D004591:Electrophoresis, Polyacrylamide Gel; D008937:Mitomycins / Q000032:analysis / Q000493:pharmacokinetics*; D008969:Molecular Sequence Data; D009707:Nucleosomes / Q000187:drug effects* / Q000378:metabolism; D013950:Thymus Gland / Q000187:drug effects / Q000378:metabolism
D003432:Cross-Linking Reagents; D008937:Mitomycins; D009707:Nucleosomes; D004247:DNA; C014675:N-methylmitomycin A