Effect of nitrendipine, a calcium antagonist, on the distribution of calcium in aortic smooth muscle cells of deoxycorticosterone-hypertensive rats. A quantitative ultracytochemical study.
Rats were made hypertensive by implantation of a pellet of deoxycorticosterone (DOC). A low dose (1 mg/kg twice daily) of the calcium antagonist nitrendipine protects against the increase in total and ionic levels of calcium in the aorta produced by the elevated blood pressure, dissociating at least in part the hypertension from the rise in aortic calcium. Ionic (free) calcium was demonstrated in aortic smooth muscle cells by the pyroantimonate ultra-cytochemical method and the electron opaque reaction product quantitated by stereological techniques. As compared to the control group, nitrendipine did not increase the number of vesicles/micron with precipitate located adjacent to the sarcolemma. DOC however increased the number of subsarcolemmal vesicles with electron opaque precipitate and sarcoplasmic calcium. Nitrendipine administration to DOC-treated rats decreased the number of vesicles to that found in the control or nitrendipine-treated group while ionic calcium in the nitrendipine + DOC group was intermediate between the control or nitrendipine group and the DOC group. The total content of calcium measured by atomic absorption correlates with the observations of ionic calcium levels demonstrated ultracytochemically. Aortic dry weights of the DOC and DOC + nitrendipine groups were comparable and significantly greater than those in the control or nitrendipine groups.
Nickerson
P A
PA
Department of Pathology, State University of New York, Buffalo.
Yang
F
F
D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D002118:Calcium / Q000378:metabolism*; D003900:Desoxycorticosterone; D005260:Female; D006651:Histocytochemistry; D006973:Hypertension / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D008854:Microscopy, Electron; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D009568:Nitrendipine / Q000494:pharmacology*; D051381:Rats
D003900:Desoxycorticosterone; D009568:Nitrendipine; D002118:Calcium