Reduction in the level of intracellular myo-inositol in cultured soybean (Glycine max) cells inhibits cell division.
Although myo-inositol is included in media for the successful growth of plant tissues, the actual requirement of most tissues, including soybean (Glycine max) callus in suspension culture, for myo-inositol has not been demonstrated. We have made use of deoxyglucose to reduce intracellular levels of myo-inositol. Deoxyglucose is phosphorylated to deoxyglucose 6-phosphate, which inhibits L-myo-inositol 1-phosphate synthase, an important enzyme in the synthesis of myo-inositol. Addition of deoxyglucose to the medium resulted in a decrease in the intracellular level of myo-inositol that corresponded with a decrease in cell division. Cell viability was not affected. When myo-inositol was added to cells along with deoxyglucose, cell division was restored, as were intracellular levels of myo-inositol. Addition of myo-inositol had no affect on the uptake or metabolism of deoxyglucose. From these results we propose that myo-inositol has a role in maintaining cell division in soybean callus tissue in suspension culture.
Biffen
M
M
Botany School, University of Cambridge, U.K.
Hanke
D E
DE
D002455:Cell Division; D002478:Cells, Cultured; D002854:Chromatography, Paper; D003847:Deoxyglucose / Q000378:metabolism; D007294:Inositol / Q000378:metabolism*; D007700:Kinetics; D013025:Glycine max / Q000166:cytology / Q000378:metabolism
D007294:Inositol; D003847:Deoxyglucose