title: Phagocyte function in juvenile periodontitis.
abstract: We studied the chemotaxis of peripheral blood polymorphonuclear leukocytes (PMNs) and monocytes and the production of tumor necrosis factor alpha by monocytes of patients with juvenile periodontitis (JP). As a group, the patients' PMNs showed significantly increased chemotaxis determined by counting the number of migrating cells within a 3-microns-pore-size filter. Determined as distance of migration within the filter, as chemotactic increment based on checkerboard analysis, as leukotactic index calculated on the basis of distance of migration and cell count at different depths within a 3-microns-pore-size filter, as distance of migration under agarose, and as the number of PMNs migrating across a 5-microns-pore-size filter, the chemotactic migration rates of PMNs of patients were similar to those of controls. Evaluation of the data on an individual basis suggested that in terms of PMN chemotaxis some patients were hyperresponsive and some were hyporesponsive. Chemotaxis, spontaneous migration, and the rates of lipopolysaccharide-induced tumor necrosis factor alpha production by JP monocytes were similar to those of control cells. Our results give credence to the view that there are minor aberrations in the functions of JP phagocytes, but the extent to which these aberrations are relevant to accumulation of PMNs at sites of infection and inflammation in vivo and possibly contribute to the pathogenesis of JP remains unclear.
authors.affiliation: Department of Bacteriology and Immunology, University of Helsinki, Finland.
authors.forename: H
authors.initials: H
authors.lastname: Repo
authors.forename: L
authors.initials: L
authors.lastname: Saxén
authors.forename: M
authors.initials: M
authors.lastname: Jäättelä
authors.forename: M
authors.initials: M
authors.lastname: Ristola
authors.forename: M
authors.initials: M
authors.lastname: Leirisalo-Repo
mesh_terms: D000293:Adolescent; D000328:Adult; D002634:Chemotaxis, Leukocyte; D005260:Female; D006801:Humans; D008297:Male; D009000:Monocytes / Q000276:immunology*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000276:immunology*; D010518:Periodontitis / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D015054:Zymosan / Q000494:pharmacology
chemical_list: D014409:Tumor Necrosis Factor-alpha; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015054:Zymosan