Identification of extracellular proteins in the rat cumulus oophorus.
We have examined the proteins associated with the mucous matrix of the rat cumulus oophorus and compared them to the composition of rat serum, follicular fluid, ampullary fluid, and oocyte-cumulus cell extract. The cumulus matrix was dispersed using Streptomyces hyaluronidase, and the proteins were analyzed by high-resolution two-dimensional polyacrylamide gel electrophoresis and compared with proteins of the serum, proestrous follicular fluid, and postovulatory ampullary fluid and extracts of oocytes and cumulus cells. In addition to albumin and transferrin, which were common to all the fluids analyzed, the cumulus material contained many proteins in common with the follicular fluid and the ampullary fluid. However, the protein extract of the cumulus matrix also contained four major proteins not present in the other fluids analyzed. Two of these proteins were acidic and heterogenous in charge and size (MW approximately 81,000 and 100,000). The other two proteins were more basic and occurred at MW approximately 90,000 and 150,000. Our results show that the extracellular matrix of the cumulus contains proteins that are not present in the fluids that surround the oocyte.
Virji
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Population Council, New York, New York 10021.
Phillips
D M
DM
Dunbar
B S
BS
D000818:Animals; D004527:Egg Proteins / Q000302:isolation & purification*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005306:Fertilization; D006821:Hyaluronoglucosaminidase / Q000302:isolation & purification; D007525:Isoelectric Focusing; D009865:Oocytes / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D013302:Streptomyces / Q000201:enzymology
D004527:Egg Proteins; D006821:Hyaluronoglucosaminidase