title: Comparison of neuraminidases of the same subtype but from different species using a new method of titration.
abstract: Neuraminidase is one of the two surface glycoproteins of influenza virions. In order to compare neuraminidases of the same subtype but isolated from different species (man, birds, pig), a new and simple method was adapted and optimized using peanut hemagglutinin. Results were very similar to those obtained with the classical method recommended by the WHO, using fetuin as a substrate. The technique was used to examine the relationship between animal and human neuraminidases belonging to serotypes N1 and N2. The results confirm the possible role of ducks as a reservoir for influenza viruses and the eventuality of interspecific exchanges.
authors.affiliation: Unit of Viral Ecology, Pasteur Institute, Paris, France.
authors.forename: B
authors.initials: B
authors.lastname: Fiszon
authors.forename: C
authors.initials: C
authors.lastname: Hannoun
mesh_terms: D000818:Animals; D000940:Antigenic Variation / Q000276:immunology; D004372:Ducks; D006801:Humans; D009980:Influenza A virus / Q000145:classification / Q000201:enzymology; D037102:Lectins; D009439:Neuraminidase / Q000032:analysis* / Q000276:immunology; D009975:Orthomyxoviridae / Q000145:classification / Q000201:enzymology*; D019887:Peanut Agglutinin; D013045:Species Specificity; D013552:Swine
chemical_list: D037102:Lectins; D019887:Peanut Agglutinin; D009439:Neuraminidase