Effects of hypothermia on testicular ischemia.
The ischemic effects of prolonged testicular torsion have been well documented; however, prevention or arrest of the damaging effects of prolonged ischemia has been incompletely studied. Two groups of Sprague-Dawley rats were subjected to varying lengths of bilateral testicular ischemia. Group I underwent normothermic ischemia for two, four, and six hours. Likewise, Group II underwent similar time periods of ischemia, however, after thirty minutes of normothermic ischemia the scrotum of each animal was placed into an ice bath maintained at 4C. Two weeks postoperatively, bilateral orchiectomy was performed. Histology of the testes of the two groups was compared. Neither group revealed significant destruction of the germinal epithelium after two hours of ischemia. Group I revealed only 25% preservation of the germinal epithelium at four hours and only 8% preservation at six hours of ischemia. In contrast, Group II which received ice showed 90% preservation of germinal epithelium at four hours and 85% preservation at six hours of ischemia. We conclude that external ice application significantly preserves seminiferous tubules at four and six hours of ischemic injury in the rat testicle.
Miller
D C
DC
Division of Urology, Medical College of Ohio, Toledo 43699.
Peron
S E
SE
Keck
R W
RW
Kropp
K A
KA
D000818:Animals; D007036:Hypothermia, Induced / Q000379:methods; D007511:Ischemia / Q000209:etiology / Q000473:pathology / Q000628:therapy*; D008297:Male; D009919:Orchiectomy; D051381:Rats; D011919:Rats, Inbred Strains; D013086:Spermatic Cord Torsion / Q000150:complications / Q000473:pathology / Q000628:therapy; D013737:Testis / Q000098:blood supply* / Q000473:pathology; D013997:Time Factors