[Application of derivative spectrophotometry for determining plasma hemoglobin and semi-quantitative evaluation of urinary porphyrins].
We described a method using second-derivative spectrophotometry with a spectrophotometer Uvikon (Kontron) for the assay of plasma hemoglobin and urinary porphyrins. We evaluated the procedure for sensitivity, linearity, analytical recovery. Non specific interference is not significant and reproductibility is excellent. This simple, rapid, sensitive, not expansive method can be adapted for use in a clinical laboratory.
Iordache
A
A
Laboratoire de Biochimie, Hôpital Pasteur, Centre Hospitalier Régional de Nice, France.
Beamonte
A
A
Soummer
A M
AM
Chambon
P
P
D006801:Humans; D010108:Oxyhemoglobins / Q000032:analysis*; D011166:Porphyrins / Q000652:urine*; D013053:Spectrophotometry / Q000379:methods*
D010108:Oxyhemoglobins; D011166:Porphyrins
Application de la spectrophotométrie dérivée au dosage de l'hémoglobine plasmatique et a l'évaluation semi-quantitative des porphyrines urinaires.