Purification and characterization of bioactive peptides from skin extracts of Rana esculenta.
The peptide fraction extracted by methanol from the skin of Rana esculenta, a species widely distributed in Western Europe, was investigated. The pharmacological activity found in the extract is attributable to the presence of authentic bradykinin, together with a shorter, partially active version of this molecule, des-Arg9-bradykinin. Also the bradykinin fragment 1-7 has been isolated, but it was inactive in our bioassay system. Moreover, a family of hydrophobic peptides has been purified and characterized, which appeared devoid of pharmacological activities when tested on smooth muscle preparations, but were provided with hemolytic activities.
Simmaco
M
M
Dipartimento di Scienze Biochimiche, Università La Sapienza, Rome, Italy.
De Biase
D
D
Severini
C
C
Aita
M
M
Erspamer
G F
GF
Barra
D
D
Bossa
F
F
D000595:Amino Acid Sequence; D000818:Animals; D001920:Bradykinin / Q000031:analogs & derivatives / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology; D011487:Protein Conformation; D011893:Rana esculenta / Q000378:metabolism*; D012867:Skin / Q000032:analysis*
D010455:Peptides; C063515:bioactive peptide A1, Rana esculenta; C063523:bioactive peptide B9, Rana esculenta; C024626:bradykinin, des-Arg(9)-; D001920:Bradykinin