Rapid and sensitive detection of DNA in Southern blots with chemiluminescence.
A new chemiluminescent Southern blot procedure offers molecular biologists a safe, ultrasensitive and rapid alternative to conventional 32P-based systems. This new DNA detection system, SOUTHERN-LIGHT, has been developed by Tropix, Inc. The luminescent signal is produced from a direct chemiluminescent substrate, disodium 3-(4-methoxyspiro[1,2-dioxetane-3-2'-tricyclo-[3.3.1.1 .3,7]decan]-4-yl) phenyl phosphate (AMPPD), which decomposes upon dephosphorylation with alkaline phosphatase. SOUTHERN-LIGHT is an ultrasensitive, rapid detection kit for use with membrane-bound DNA. It is the first test kit to incorporate AMPPD. It requires no specialized equipment and results can be conveniently imaged on instant film or x-ray film within 5-60 min of exposure.
Bronstein
I
I
Hospital of the University of Pennsylvania.
Voyta
J C
JC
Lazzari
K G
KG
Murphy
O
O
Edwards
B
B
Kricka
L J
LJ
D000218:Adamantane / Q000031:analogs & derivatives; D001710:Biotin / Q000031:analogs & derivatives; D015139:Blotting, Southern; D004247:DNA / Q000032:analysis*; D015342:DNA Probes; D003856:Deoxyuracil Nucleotides; D008163:Luminescent Measurements
D015342:DNA Probes; D003856:Deoxyuracil Nucleotides; C061523:3-(2'-spiroadamantane)-4-methoxy-4-(3''-phosphoryloxy)phenyl-1,2-dioxetane; D001710:Biotin; C045931:biotin-11-dUTP; D004247:DNA; D000218:Adamantane