Intrathecal synthesis of anti-HIV IgG: correlation with increasing duration of HIV-1 infection.
We determined intrathecal synthesis (ITS) of anti-HIV-1 immunoglobulin in 62 CSF samples from 51 HIV-1 seropositive homosexual men using an ELISA technique with paired serum and CSF samples diluted to a concentration of IgG of 10 micrograms/ml. All subjects were neurologically normal and none was taking zidovudine. We estimated duration of HIV-1 infection from semiannual serologic testing during the 3-year period before CSF analysis and detected ITS of anti-HIV-1 immunoglobulin in 2 of 12 (17%) of those with less than 18 months of HIV-1 seropositivity, in 3 of 21 (14%) with 19 to 36 months, and in 13 of 29 (45%) with greater than 36 months of HIV-1 seropositivity (p = 0.037). There was a trend toward an inverse relationship between level of ITS and the peripheral blood T-helper lymphocyte count. This study demonstrates that increasing ITS of anti-HIV-1 IgG is related to duration of HIV-1 infection and suggests an inverse correlation with systemic immune status. The detection of ITS of anti-HIV-1 immunoglobulin is not necessarily a marker of clinically overt neurologic involvement.
Van Wielink
G
G
Johns Hopkins Hospital, Baltimore, MD 21205.
McArthur
J C
JC
Moench
T
T
Farzadegan
H
H
McArthur
J H
JH
Johnson
R T
RT
Saah
A
A
D000328:Adult; D000704:Analysis of Variance; D016009:Chi-Square Distribution; D004797:Enzyme-Linked Immunosorbent Assay; D015483:HIV Antibodies / Q000134:cerebrospinal fluid*; D015658:HIV Infections / Q000134:cerebrospinal fluid* / Q000276:immunology; D006679:HIV Seropositivity / Q000134:cerebrospinal fluid* / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000134:cerebrospinal fluid*; D008297:Male; D008875:Middle Aged
D015483:HIV Antibodies; D007074:Immunoglobulin G