Morphological recovery of axotomized goldfish retinal ganglion cells in an environment known to prevent retinotopic refinement of their regenerated tectal arbors.
Axonal injury provokes well-characterized morphological changes in goldfish retinal ganglion cells. These reach a peak as the regenerating axons restore a grossly retinotopic projection map to the tectum, and then regress as the map is refined by a mechanism involving locally-correlated activity. The aim of this study was to look for any interdependence between morphological recovery and retinotopic refinement. Stroboscopic light was used to keep regenerated optic arbors in non-retinotopic locations for 70 days after optic nerve cut and lens ablation. Controls were kept in constant or diurnal light, both of which allow refinement of the retinotectal map. Nucleolar frequency, perikaryal area and nuclear area were used as indices of neuronal recovery, and ganglion cell counts were performed. After 35 days in diurnal light, the nucleoli of axotomized cells had increased in size, prominence and number, and both nucleus and cytoplasm had roughly doubled in area. After 70 days, these features had almost returned to normal not only in diurnal and constant light but in stroboscopic light as well. A small but significant cell loss, averaging 13.4-14.7%, was seen after optic nerve cut and lens ablation regardless of stage in regeneration (35 or 70 days) or lighting. Evidently, morphological recovery is independent of retinotopic refinement, which is known to be no further advanced after 70 days in stroboscopic light than after 35 days in diurnal light.
Cook
J E
JE
Department of Anatomy and Developmental Biology, University College London, U.K.
D000818:Animals; D002452:Cell Count; D003530:Cyprinidae / Q000502:physiology*; D006054:Goldfish / Q000502:physiology*; D008029:Lighting; D009416:Nerve Regeneration; D009473:Neuronal Plasticity; D012160:Retina / Q000502:physiology*; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000502:physiology*; D013477:Superior Colliculi / Q000166:cytology / Q000502:physiology*