title: Rapid procedure to determine the DNA base composition from small amounts of gram-positive bacteria.
abstract: A universal rapid procedure to determine the DNA base composition (mol% guanine + cytosine) of Gram-positive bacteria is described. Cells of Gram-positive bacteria were lysed with achromo-peptidase and the mol% G + C of their DNAs were determined by using high performance liquid chromatography. One ml of a Gram-positive bacterial suspension which matched MacFarland No. 3 standard turbidity was sufficient to determine the mol% G + C within 3 h.
authors.affiliation: Department of Microbiology, Gifu University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ezaki
authors.forename: S M
authors.initials: SM
authors.lastname: Saidi
authors.forename: S L
authors.initials: SL
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: H
authors.initials: H
authors.lastname: Yamamoto
authors.forename: E
authors.initials: E
authors.lastname: Yabuuchi
mesh_terms: D001482:Base Composition; D002851:Chromatography, High Pressure Liquid; D004269:DNA, Bacterial / Q000032:analysis*; D006094:Gram-Positive Bacteria / Q000032:analysis*; D012697:Serine Endopeptidases
chemical_list: D004269:DNA, Bacterial; D012697:Serine Endopeptidases; C036813:lysyl endopeptidase