Purification and properties of buffalo serum albumin.
Based on a detailed study of the solubility of serum albumin, a procedure for its purification by selective ammonium sulphate precipitation has been developed. Using buffalo serum, first extraneous proteins were precipitated by making the serum 2.26 M saturated with ammonium sulphate at pH 7.0 and then albumin was precipitated from the supernatant at 1.9 M ammonium sulphate concentration at pH 4.2. The overall yield of serum albumin thus isolated was about 55% with a purity of 97%. The protein preparation gave a single nearly symmetrical peak on Sephadex G-100 column and virtually a single band on polyacrylamide gel electrophoresis in the presence and absence of SDS. Buffalo serum albumin has a molecular weight of 69,000 Da. The hydrodynamic properties such as Stoke's radius (3.70 nm), diffusion coefficient (6.03 X 10(-7) cm2/s) and frictional ratio (1.36) obtained by analytical gel chromatography, bilirubin binding characteristics and its interaction with anti-bovine serum albumin antiserum suggest that buffalo serum albumin is very similar to BSA in its molecular properties.
Tayyab
S
S
Department of Biochemistry, Faculty of Medicine, Aligarh, Muslim University, India.
Qasim
M A
MA
D000645:Ammonium Sulfate; D000818:Animals; D001663:Bilirubin / Q000378:metabolism; D002020:Buffaloes / Q000097:blood*; D002417:Cattle; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005590:Fractional Precipitation; D007106:Immune Sera; D008970:Molecular Weight; D012709:Serum Albumin / Q000302:isolation & purification* / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000276:immunology; D013057:Spectrum Analysis
D007106:Immune Sera; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D001663:Bilirubin; D000645:Ammonium Sulfate