title: Determination of excretion of inulin, creatinine, sodium sulfanilate, and phenolsulfonphthalein to assess renal function in goats.
abstract: Excretion of creatinine, sodium sulfanilate (SS), and phenolsulfonphthalein (PSP) was studied in healthy goats. In conscious goats, mean (+/- SEM) inulin clearance was 2.26 +/- 0.08 ml/min/kg of body weight. Endogenous creatinine clearance, 1.97 +/- 0.09 ml/min/kg, underestimated inulin clearance (P less than 0.01), probably because of the presence of noncreatinine chromogens in caprine plasma. The estimated renal clearance of PSP was 6.88 +/- 0.39 ml/min/kg, whereas the estimated renal clearance of SS was 3.71 +/- 0.39 ml/min/kg. Both exceeded inulin clearance (P less than 0.01), confirming renal tubular secretion of both compounds. In 6 anesthetized goats, exogenous creatinine clearance and SS clearance exceeded inulin clearance (P less than 0.05). Results of stop-flow experiments documented secretion of creatinine and SS by the proximal portion of the caprine nephron. Plasma half-life of PSP in uninephrectomized goats exceeded that in intact goats (20.2 +/- 1.5 min vs 11.9 +/- 0.7 min; P less than 0.01). Similarly, plasma half-life of SS was greater in goats after uninephrectomy (58.2 +/- 6.2 min vs 30.4 +/- 1.2 min; P less than 0.01).
authors.affiliation: Department of Physiology, College of Veterinary Medicine, University of Georgia, Athens 30602.
authors.forename: S A
authors.initials: SA
authors.lastname: Brown
authors.forename: C
authors.initials: C
authors.lastname: Groves
authors.forename: J A
authors.initials: JA
authors.lastname: Barsanti
authors.forename: D R
authors.initials: DR
authors.lastname: Finco
mesh_terms: D000818:Animals; D003404:Creatinine / Q000097:blood / Q000652:urine*; D005260:Female; D006041:Goats / Q000097:blood / Q000502:physiology* / Q000652:urine; D006207:Half-Life; D007444:Inulin / Q000097:blood / Q000652:urine*; D007668:Kidney / Q000502:physiology*; D008297:Male; D008657:Metabolic Clearance Rate; D009392:Nephrectomy / Q000662:veterinary; D010635:Phenolphthaleins / Q000032:analysis*; D010637:Phenolsulfonphthalein / Q000032:analysis*; D011188:Potassium / Q000097:blood / Q000652:urine; D012964:Sodium / Q000097:blood / Q000652:urine; D013997:Time Factors
chemical_list: D010635:Phenolphthaleins; D007444:Inulin; D012964:Sodium; D003404:Creatinine; D010637:Phenolsulfonphthalein; D011188:Potassium