Effect of different fixatives on Con A surface receptors of mouse peritoneal macrophages.
The effects of glutaraldehyde, formaldehyde, or osmium tetroxide fixation on the number of labeled Con A surface receptors on mouse peritoneal macrophages were compared. Gold-labeled Con A receptors were found to be isolatedly arranged and evenly distributed on cell surfaces independent of the fixative used. Only cells preincubated with Con A and subsequently fixed by osmium tetroxide showed arrangement of labeled receptors in clusters. Significant differences were found in the number of Con A receptors per cell depending on the fixative used. The fluorescence intensity of FITC-Con A staining was detected spectrophotometrically, the characteristic X-rays of gold-labeled Con A receptors were determined by means of electron beam-induced X-ray microanalysis. The experimental results obtained both at light and electron microscopic level pointed to formaldehyde being the best fixative also for this purpose.
Peschke
T
T
GDR Academy of Sciences, Central Institute of Microbiology and Experimental Therapy, DDR, Jena.
Wollweber
L
L
Gabert
A
A
Augsten
K
K
Stracke
R
R
D000818:Animals; D003208:Concanavalin A / Q000031:analogs & derivatives / Q000378:metabolism; D004577:Electron Probe Microanalysis; D005404:Fixatives / Q000494:pharmacology*; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives*; D005452:Fluoresceins / Q000378:metabolism; D007150:Immunohistochemistry; D008264:Macrophages / Q000648:ultrastructure*; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D009993:Osmium Tetroxide; D010529:Peritoneal Cavity; D011952:Receptors, Concanavalin A / Q000187:drug effects* / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D005404:Fixatives; D005452:Fluoresceins; D011952:Receptors, Concanavalin A; C042784:fluorescein isothiocyanate-concanavalin A; D003208:Concanavalin A; D016650:Fluorescein-5-isothiocyanate; D009993:Osmium Tetroxide