title: Diisopropylfluorophosphate-binding proteins in the outer membrane of Fusobacterium nucleatum: strain variations.
abstract: Six strains of Fusobacterium nucleatum were tested for their ability to react with [3H]diisopropylfluorophosphate (DFP), a serine protease inhibitor. Several cytoplasmic proteins were labelled but the strongest labelling was regularly observed in a few outer membrane proteins. The number and the molecular mass of the proteins detected varied according to the strain tested. A 61 kDa protein was labelled in all strains tested, including the two type strains ATCC 10953 and ATCC 25586. A 65 kDa protein was particularly strongly labelled in strains Fev1 and F6.
authors.affiliation: Department of Biochemistry, University of Bergen, Norway.
authors.forename: K A
authors.initials: KA
authors.lastname: Brokstad
authors.forename: V
authors.initials: V
authors.lastname: Bakken
authors.forename: E N
authors.initials: EN
authors.lastname: Vasstrand
authors.forename: H B
authors.initials: HB
authors.lastname: Jensen
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D005673:Fusobacterium / Q000378:metabolism*; D007531:Isoflurophate / Q000378:metabolism*; D008970:Molecular Weight; D013045:Species Specificity
chemical_list: D001425:Bacterial Outer Membrane Proteins; D007531:Isoflurophate