Production and characterization of a monoclonal antibody against neopterin.
We have produced and characterized the first monoclonal antibody against neopterin (D-erythro-6-(1,2,3,-trihydroxypropyl)pterin). The antibody specifically recognizes neopterin in a modified RIA. The binding capacity in this assay is 34%, the sensitivity limit of inhibition is 0.9 nmol/l. Cross-reactivity exists with monapterin (L-threo(1,2,3,trihydroxypropyl)pterin) in 30%, with other pteridines cross-reactivity has been found in less than 5%.
Weyrer
W
W
Ludwig-Boltzmann-Institut für AIDS-Forschung, Innsbruck, Austria.
Möst
J
J
Sölder
B
B
Fuchs
D
D
Wachter
H
H
Dierich
M P
MP
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000918:Antibody Specificity; D001708:Biopterins / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D019798:Neopterin; D011621:Pteridines / Q000276:immunology; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D011621:Pteridines; D001708:Biopterins; D019798:Neopterin