title: Multiple peptide synthesis using a single support (MPS3).
abstract: An automated multiple peptide synthesis method to synthesize, cleave, and purify several peptides simultaneously in a single batch has been developed. The technique is based on the synthesis of multiple peptides on a single solid phase support and is easily adapted to manual or to automated methods. The approach relies on coupling of amino acid mixtures to the resin and it has been found that DCC/HOBt gives the best coupling performance. Fast Atom Bombardment Mass Spectrometry (FAB-MS) was used to rapidly and efficiently identify the peptides in each synthetic mixture which significantly assisted the purification process by HPLC. The method has been successfully applied to the synthesis of magainin 2 and angiotensinogen peptides.
authors.affiliation: Department of Biological Sciences, Monsanto Company, Chesterfield, MO.
authors.forename: F S
authors.initials: FS
authors.lastname: Tjoeng
authors.forename: D S
authors.initials: DS
authors.lastname: Towery
authors.forename: J W
authors.initials: JW
authors.lastname: Bulock
authors.forename: D E
authors.initials: DE
authors.lastname: Whipple
authors.forename: K F
authors.initials: KF
authors.lastname: Fok
authors.forename: M H
authors.initials: MH
authors.lastname: Williams
authors.forename: M E
authors.initials: ME
authors.lastname: Zupec
authors.forename: S P
authors.initials: SP
authors.lastname: Adams
mesh_terms: D000595:Amino Acid Sequence; D000808:Angiotensinogen; D000818:Animals; D023181:Antimicrobial Cationic Peptides; D055598:Chemical Phenomena; D002621:Chemistry; D054806:Magainins; D008969:Molecular Sequence Data; D010455:Peptides; D029867:Xenopus Proteins; D014982:Xenopus laevis
chemical_list: D023181:Antimicrobial Cationic Peptides; D054806:Magainins; D010455:Peptides; D029867:Xenopus Proteins; C053301:magainin 2 peptide, Xenopus; D000808:Angiotensinogen