title: N-acetylglucosamine-6-phosphate deacetylase in hepatocytes, Kupffer cells and sinusoidal endothelial cells from rat liver.
abstract: The activity of N-acetylglucosamine-6-phosphate deacetylase, a key enzyme in the pathway of N-acetylglucosamine catabolism, was measured in hepatocytes, Kupffer cells and sinusoidal endothelial cells from rat liver and cultured human skin fibroblasts. Kupffer cells and endothelial cells had similar high levels of deacetylase activity that were more than twice the level observed in fibroblasts. In contrast, hepatocytes had extremely low activity (several hundredfold less than Kupffer cells and endothelial cells). A major implication of deacetylase deficiency in hepatocytes is that N-acetylglucosamine generated as a result of the catabolism of complex carbohydrates in these cells cannot enter glycolysis and must be largely reused for the synthesis of plasma glycoproteins and other N-acetylglucosamine-containing macromolecules.
authors.affiliation: School of Dentistry, University of Alabama, Birmingham 35294.
authors.forename: P
authors.initials: P
authors.lastname: Campbell
authors.forename: J N
authors.initials: JN
authors.lastname: Thompson
authors.forename: J R
authors.initials: JR
authors.lastname: Fraser
authors.forename: T C
authors.initials: TC
authors.lastname: Laurent
authors.forename: H
authors.initials: H
authors.lastname: Pertoft
authors.forename: L
authors.initials: L
authors.lastname: Rodén
mesh_terms: D000581:Amidohydrolases / Q000378:metabolism*; D000818:Animals; D004730:Endothelium, Vascular / Q000201:enzymology*; D007728:Kupffer Cells / Q000201:enzymology*; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D051381:Rats
chemical_list: D000581:Amidohydrolases; C018717:N-acetylglucosamine-6-phosphate deacetylase