Sialyllacto-N-fucopentaose II (sialylated Le(a)) coupled to humanserum albumin used as standard in immunoassays of tumour associated antigens CA 19-9 and CA-50 in serum.
Penta and hexasaccharides from human milk, containing sialic acid and/or fucose residues were conjugated to human serum albumin and used as antigens in three different methods for CA 19-9, two methods for CA-50 and one method for CA-195. In accordance with reported results, the 19-9, CC 3C 195 and C-50 monoclonal antibodies were found to react with the sialyllacto-N-fucopentaose II conjugate, although to a different extent. A negligible reaction was observed with the isomeric conjugate, sialyllacto-N-fucopentaose III, and no reaction was seen with the sialyllacto-N-tetraose conjugate. The use of defined oligosaccharide conjugates for the standardization of assays of tumour markers in serum is discussed.
Masson
P
P
Department of Clinical Chemistry, University Hospital, Lund, Sweden.
Lundblad
A
A
Wieslander
J
J
D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000592:standards*; D009844:Oligosaccharides; D012015:Reference Standards; D012709:Serum Albumin
D015295:Antigens, Tumor-Associated, Carbohydrate; D009844:Oligosaccharides; D012709:Serum Albumin; C058981:sialyl-Le(a) oligosaccharide