Infrared spectra of phospholipid membranes: interfacial dehydration by volatile anesthetics and phase transition.
Fourier-transform infrared attenuated total reflection (ATR) spectroscopy was used to study the effect of volatile anesthetics on fully hydrated dipalmitoylphosphatidylcholine (DPPC) vesicle membranes. The main phase transition was monitored by the change in the C-H2 asymmetric stretching frequencies of the lipid tails. The surface property was analyzed by the changes in the P = O stretching, (CH3)3-N+ stretching of the hydrophilic head, and C = O stretching of the glycerol skeleton. The partial pressures of those agents that decreased the transition temperature 1.0 C degree were halothane 0.75, enflurane 1.90 and CCl4 0.85 kPa. At a 2:1 lipid/anesthetic mole ratio, the polar anesthetics, halothane and enflurane, increased the ratio of (P = O stretching band area)/((CH3)3-N+ stretching band area) by 26.3% and 21.1%, respectively, whereas apolar CCl4 increased it 10.5%. The water molecules bound to the P = O moiety are apparently replaced by the anesthetic molecules. The deconvoluted C = O spectra showed two peaks: free sn-1 that is closer to the lipid core and hydrogen-bonded sn-2 that is closer to the polar head. Addition of halothane and enflurane, but not CCl4, increased the number of peaks to three. The third peak is free sn-2, formed by disrupting hydrogen-bonding to water. Because the temperature-induced spectral change was limited to C-H2 stretching at the main phase transition, the effects of anesthetics on the lipid membrane structure are not identical to temperature elevation. Among anesthetics, the effects of apolar and polar molecules on the interfacial properties are different.
Tsai
Y S
YS
Department of Anesthesia, University of Utah School of Medicine, Salt Lake City.
Ma
S M
SM
Nishimura
S
S
Ueda
I
I
D015060:1,2-Dipalmitoylphosphatidylcholine; D002251:Carbon Tetrachloride / Q000494:pharmacology*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002794:Choline; D004737:Enflurane / Q000494:pharmacology*; D005583:Fourier Analysis; D006221:Halothane / Q000494:pharmacology*; D008081:Liposomes; D010313:Partial Pressure; D010710:Phosphates; D013055:Spectrophotometry, Infrared; D013696:Temperature
D008081:Liposomes; D010710:Phosphates; D015060:1,2-Dipalmitoylphosphatidylcholine; D004737:Enflurane; D002251:Carbon Tetrachloride; D002794:Choline; D006221:Halothane