Excretion of hydrogen peroxide in human urine.
The excretion of hydrogen peroxide in human urine has been demonstrated for the first time. This was accomplished by a new radioactive method developed on the basis of decarboxylation of alpha-ketoglutaric acid by H2O2. Urine samples were incubated with alpha ketoglutarate pulsed with iwts 1-14C-analogue, and CO2 formed by decarboxylation was determined by radioactivity measurements. Blanks were prepared by pre-incubation of the samples with catalase. Both male and female subjects were studied. On an average the concentration of H2O2 in urine was approximately 100 +/- 60 microM (10(-4) M). Peroxide excretion was found to be unexpectedly high and might thus be useful for clinical diagnosis and therapy in diseases purported to be related to oxidative stress.
Varma
S D
SD
Department of Ophthalmology, School of Medicine & Dentistry, University of Maryland School of Medicine, Baltimore 21201.
Devamanoharan
P S
PS
D000328:Adult; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000652:urine*; D007202:Indicators and Reagents; D007656:Ketoglutaric Acids; D008297:Male
D007202:Indicators and Reagents; D007656:Ketoglutaric Acids; D006861:Hydrogen Peroxide