title: Hyperthermia, unlike ionizing radiation and chemical oxidative stress, does not stimulate proteolysis in erythrocytes.
abstract: 1. Oxidative stress by phenazine methosulfate stimulated proteolysis in erythrocytes. 2. Gamma-irradiation of erythrocytes in the range of 50-1000 Gy also resulted in the induction of proteolysis. 3. Though it has been suggested that hyperthermia imposes an oxidative stress on a cell, hyperthermic exposure of erythrocytes (30 min, 39-49 degrees C) did not stimulate proteolysis during subsequent incubation of whole cells or hemolysates. 4. Proteolytic degradation of spectrin was accelerated during incubation of membranes isolated from cells heated above 45 degrees C but this effect seems to be due rather to thermal denaturation of spectrin than to oxidative modification of cellular proteins by hyperthermia.
authors.affiliation: Department of Biophysics, University of Lódź, Poland.
authors.forename: G
authors.initials: G
authors.lastname: Bartosz
authors.forename: M
authors.initials: M
authors.lastname: Gaczynska
authors.forename: W
authors.initials: W
authors.lastname: Retelewska
authors.forename: E
authors.initials: E
authors.lastname: Grzelinska
authors.forename: J
authors.initials: J
authors.lastname: Rosin
mesh_terms: D000596:Amino Acids / Q000032:analysis; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D005720:Gamma Rays; D006461:Hemolysis; D006358:Hot Temperature; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D008773:Methylphenazonium Methosulfate / Q000494:pharmacology; D008954:Models, Biological; D009996:Osmotic Fragility / Q000187:drug effects / Q000528:radiation effects; D013049:Spectrin / Q000378:metabolism
chemical_list: D000596:Amino Acids; D008565:Membrane Proteins; D013049:Spectrin; D008773:Methylphenazonium Methosulfate