title: Recombinant human bone morphogenetic protein induces bone formation.
abstract: We have purified and characterized active recombinant human bone morphogenetic protein (BMP) 2A. Implantation of the recombinant protein in rats showed that a single BMP can induce bone formation in vivo. A dose-response and time-course study using the rat ectopic bone formation assay revealed that implantation of 0.5-115 micrograms of partially purified recombinant human BMP-2A resulted in cartilage by day 7 and bone formation by day 14. The time at which bone formation occurred was dependent on the amount of BMP-2A implanted; at high doses bone formation could be observed at 5 days. The cartilage- and bone-inductive activity of the recombinant BMP-2A is histologically indistinguishable from that of bone extracts. Thus, recombinant BMP-2A has therapeutic potential to promote de novo bone formation in humans.
authors.affiliation: Genetics Institute, Cambridge, MA 02140.
authors.forename: E A
authors.initials: EA
authors.lastname: Wang
authors.forename: V
authors.initials: V
authors.lastname: Rosen
authors.forename: J S
authors.initials: JS
authors.lastname: D'Alessandro
authors.forename: M
authors.initials: M
authors.lastname: Bauduy
authors.forename: P
authors.initials: P
authors.lastname: Cordes
authors.forename: T
authors.initials: T
authors.lastname: Harada
authors.forename: D I
authors.initials: DI
authors.lastname: Israel
authors.forename: R M
authors.initials: RM
authors.lastname: Hewick
authors.forename: K M
authors.initials: KM
authors.lastname: Kerns
authors.forename: P
authors.initials: P
authors.lastname: LaPan
mesh_terms: D000818:Animals; D001846:Bone Development / Q000187:drug effects*; D019485:Bone Morphogenetic Proteins; D001842:Bone and Bones / Q000166:cytology; D002356:Cartilage / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*; D002460:Cell Line; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D008970:Molecular Weight; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D019485:Bone Morphogenetic Proteins; D006133:Growth Substances; D011506:Proteins; D011994:Recombinant Proteins