Formation of alpha-deoxyadenosine in polydeoxynucleotides exposed to ionizing radiation under anoxic conditions.
When poly(dA), poly(dA-dT), and salmon testis DNA were gamma-irradiated under nitrogen, the major deoxyadenosine damage product (excluding liberated adenine) was identified as the alpha-anomer of deoxyadenosine. The yields of alpha-deoxyadenosine from poly(dA), poly(dA-dT), and salmon testis DNA irradiated with a dose of 500 Gy under anoxic conditions were 1.5, 1.3, and 1.3%, respectively. No alpha-deoxyadenosine was detected after irradiation under oxic conditions. The presence of nucleotides with the alpha-configuration at the anomeric carbon atom in the DNA chain may have a significant effect on its tertiary structure and possibly modify its biological activity.
Lesiak
K B
KB
Department of Radiology, Wake Forest University Medical Center, Winston-Salem, North Carolina 27103.
Wheeler
K T
KT
D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000528:radiation effects; D003839:Deoxyadenosines / Q000528:radiation effects*; D005720:Gamma Rays; D009682:Magnetic Resonance Spectroscopy; D010100:Oxygen; D011061:Poly A / Q000528:radiation effects*; D011067:Poly dA-dT / Q000528:radiation effects*; D011089:Polydeoxyribonucleotides / Q000528:radiation effects*
D003839:Deoxyadenosines; D011089:Polydeoxyribonucleotides; D011061:Poly A; C015465:poly(dA); D011067:Poly dA-dT; D004247:DNA; D010100:Oxygen