Unstimulated force during hypoxia of rat cardiac muscle: stiffness and calcium dependence.
The stiffness of rat cardiac trabeculae was measured in vitro to distinguish between an increase in unstimulated force (Fu) caused by rapid cycling of cross bridges or caused by rigor bridges during hypoxia. The force was measured with a strain gauge, the sarcomere length was determined by laser diffraction techniques, and muscle length was controlled by means of a motor. Stiffness was analyzed by using small (less than 1% of muscle length) sinusoidal length perturbations of 1 and 100 Hz. The stiffness at 100 Hz increased linearly with force during tetani at a varied [Sr2+] (0.25-10 mM) in the Krebs-Henseleit (K-H) buffer, but remained virtually unchanged at 1 Hz. In contrast, the stiffness of both the passive muscle and the muscle exposed to either CN- or to PO2 less than 1.5 mmHg up to development of maximal Fu (Fumax) was similar at 1- and 100-Hz perturbations. Less profound hypoxia (PO2 6-10 mmHg) resulted in spontaneous sarcomere activity during the rise in Fu, and an increase in the ratio of stiffness at 100 Hz to stiffness at 1 Hz was detected. When oxidative phosphorylation was inhibited by CN- (2 mM) while the muscle was stimulated in the absence of both Ca2+ and Na+ (choline+substituted), the addition of Na+ at the time at which Fu had reached 30-40% of Fumax did not affect the rate of rise of Fu. These results show that the development of Fu during more complete anoxia in rat trabeculae is completely due to the formation of rigor links and that Ca2(+)-dependent cross-bridge activation can contribute to the rise in Fu during less severe hypoxia.
Leijendekker
W J
WJ
Department of Medicine, Health Sciences Center, University of Calgary, Alberta, Canada.
Gao
W D
WD
ter Keurs
H E
HE
D000818:Animals; D000838:Anions; D002118:Calcium / Q000378:metabolism / Q000502:physiology*; D003486:Cyanides; D004548:Elasticity; D004558:Electric Stimulation; D005260:Female; D006321:Heart / Q000503:physiopathology*; D000860:Hypoxia / Q000139:chemically induced / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D009200:Myocardial Contraction; D009206:Myocardium / Q000378:metabolism; D009584:Nitrogen; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000494:pharmacology
D000838:Anions; D003486:Cyanides; D012964:Sodium; D009584:Nitrogen; D002118:Calcium