The mitochondrial genome organization of a maize fertile cmsT revertant line is generated through recombination between two sets of repeats.
The mitochondrial genome (mtDNA) organization from a fertile revertant line (V3) derived from the maize cytoplasmic male sterile type T (cmsT) callus tissue culture has been determined. We report that the sequence complexity can be mapped on to a circular "master chromosome" of 705 kb which includes a duplication of 165 kb of DNA when compared to its male sterile progenitor. Associated with this event is also a 0.423-kb deletion, which removed the cmsT-associated urf13 gene. As found for the maize normal type (N) and cmsT mitochondrial genomes, the V3 master chromosome also exists as a multipartite structure generated by recombination through repeated sequences.
Fauron
C M
CM
Howard Hughes Medical Institute, University of Utah, Salt Lake City 84132.
Havlik
M
M
Brettell
R I
RI
D004272:DNA, Mitochondrial / Q000235:genetics*; D008957:Models, Genetic; D009693:Nucleic Acid Hybridization; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D003313:Zea mays / Q000235:genetics* / Q000502:physiology
D004272:DNA, Mitochondrial