Fibre-optic biosensor based on luminescence and immobilized enzymes: microdetermination of sorbitol, ethanol and oxaloacetate.
We have investigated highly selective and ultrasensitive biosensors based on luminescent enzyme systems linked to optical transducers. A fibre-optic sensor with immobilized enzymes was designed; the solid-phase bioreagent was maintained in close contact contact with the tip of a glass fibre bundle connected to the photomultiplier tube of a luminometer. A bacterial luminescence fibre-optic sensor was used for the microdetermination of NADH. Various NAD(P)-dependent enzymes, sorbitol dehydrogenase, alcohol dehydrogenase and malate dehydrogenase, were co-immobilized on preactivated polyamide membranes with the bacterial system and used for the microdetermination of sorbitol, ethanol and oxaloacetate at the nanomolar level with a good precision.
Gautier
S M
SM
Laboratoire de Génie Enzymatique-Atelier de Biotechnologie, CNRS-Université Claude Bernard Lyon, Villeurbanne, France.
Blum
L J
LJ
Coulet
P R
PR
D015374:Biosensing Techniques; D004800:Enzymes, Immobilized; D000431:Ethanol / Q000032:analysis; D005336:Fiber Optic Technology; D008163:Luminescent Measurements; D008832:Microchemistry; D009243:NAD / Q000032:analysis; D010071:Oxaloacetates / Q000032:analysis; D013012:Sorbitol / Q000032:analysis
D004800:Enzymes, Immobilized; D010071:Oxaloacetates; D009243:NAD; D000431:Ethanol; D013012:Sorbitol