The relevance of the order of revascularization in liver grafting.
A study was conducted comparing the effects of initial arterialization vs. initial portal revascularization in unstored warm ischemic (for 30 min) livers, livers stored for 4 hr at 0 degrees C in Collins solution and livers rendered warm ischemic for 1 hr before removal and replacement as autografts. All livers that received initial arterialization showed uniform diffuse perfusion, whereas those with initial portal perfusion were patchy and well perfused only in the right lobe. In the experimental animal, initial arterialization using an end-to-end method was much easier and required less retraction. The energy charge and ATP levels dropped sharply during brief warm ischemia but returned rapidly to normal on revascularization. Although the decline in energy charge was less in stored livers, the return to normal was slower and incomplete. Plasma levels of AST indicated much greater damage in the stored livers and were lowest in recipients in unstored livers that were arterialized first. After longer warm ischemia, energy charge values declined and only completely returned to 60% of preoperative values within 2 hr of grafting. Despite this, the survival rate of these animals was very poor and only one survived overnight. Seven of the 12 survived the procedure, but in five death occurred within 30 min of full revascularization. In this group, AST levels rose sharply after revascularization to a mean level of 1,000 U.(ABSTRACT TRUNCATED AT 250 WORDS)
Hickman
R
R
Department of Surgery, University of Cape Town, South Africa.
Innes
C R
CR
D000136:Acid-Base Equilibrium; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001158:Arteries / Q000502:physiology; D001219:Aspartate Aminotransferases / Q000097:blood; D001786:Blood Glucose / Q000032:analysis; D006493:Heparin / Q000378:metabolism; D007511:Ischemia / Q000503:physiopathology*; D008099:Liver / Q000098:blood supply*; D016031:Liver Transplantation / Q000502:physiology*; D009926:Organ Preservation; D011168:Portal System / Q000502:physiology; D013552:Swine; D013696:Temperature
D001786:Blood Glucose; D000255:Adenosine Triphosphate; D006493:Heparin; D001219:Aspartate Aminotransferases