Extraction of 6-monoacetylmorphine from urine.
An efficient and improved procedure for the extraction of 6-monoacetylmorphine (6-MAM) from urine is described. The procedure uses a 10% isobutanol in methylene chloride solution for initial extraction of 6-MAM, back extraction with acidic sodium acetate, and then another alkaline extraction into 10% isobutanol in methylene chloride. Compared to solid-phase extraction, this method provided significant improvement in 6-MAM recovery, along with the elimination of extraneous peaks when analyzed by GC/MS.
Romberg
R W
RW
Navy Drug Screening Laboratory, Great Lakes, Illinois 60088.
Brown
V E
VE
D000215:Acylation; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D009022:Morphine Derivatives / Q000652:urine*
D009022:Morphine Derivatives; C026979:6-O-monoacetylmorphine