title: A comparison of specificity and biological activity of polyclonal and monoclonal antibodies raised against Salmonella minnesota R595 lipopolysaccharide.
abstract: Murine monoclonal antibodies (MAbs) and immune rabbit serum were raised against the rough mutant of Salmonella minnesota strain R595. These antibodies were tested for their ability to inhibit LPS-induced B-cell mitogenicity and neutralise LPS toxicity in chick embryos. Immune rabbit serum inhibited both mitogenicity and LPS lethality. None of the MAbs or a cocktail of antibodies were able to neutralise LPS lethality in chick embryos. However, they were able to inhibit mitogenicity by varying degrees.
authors.affiliation: Department of Hematology/Oncology, Michael Reese Hospital and Medical Center, Chicago, IL.
authors.forename: N D
authors.initials: ND
authors.lastname: Mehta
authors.forename: B M
authors.initials: BM
authors.lastname: Wilson
authors.forename: N T
authors.initials: NT
authors.lastname: Rapson
authors.forename: C S
authors.initials: CS
authors.lastname: Easmon
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D003429:Cross Reactions / Q000276:immunology; D008070:Lipopolysaccharides / Q000032:analysis / Q000276:immunology*; D008934:Mitogens / Q000037:antagonists & inhibitors; D011863:Radioimmunoassay; D012475:Salmonella / Q000276:immunology*; D013045:Species Specificity
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D008070:Lipopolysaccharides; D008934:Mitogens