Effect of volume and dose on the pulmonary distribution of exogenous surfactant administered to normal rabbits or to rabbits with oleic acid lung injury.
Administration of exogenous lung surfactant to infants with or at risk for respiratory distress syndrome has been demonstrated to improve gas exchange and survival; administration of surfactant to patients with the adult respiratory distress syndrome is currently undergoing clinical evaluation. Although it is currently assumed the optimal effect will occur when administered surfactant is distributed homogeneously throughout the lung, little is known of the influence of variables inherent in the administration procedure on subsequent distribution. To address this question, we studied the effect of the volume size in which the surfactant is suspended for instillation, and demonstrated a marked relationship in the normal rabbit lung between this volume and the subsequent homogeneity of surfactant distribution. In the rabbit lung that was acutely injured by oleic acid, this relationship was not evident. Concentration of administered surfactant was not demonstrated to be of major influence on its distribution after administration. Our results focus attention on the importance of parameters of the administration procedure, and also demonstrate the usefulness of the techniques used for determination of surfactant distribution.
Gilliard
N
N
Department of Medicine, University of California, San Diego.
Richman
P M
PM
Merritt
T A
TA
Spragg
R G
RG
D000818:Animals; D007269:Injections, Intra-Arterial; D007322:Instillation, Drug; D008168:Lung / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008170:Lung Compliance; D009829:Oleic Acids / Q000009:adverse effects*; D010743:Phospholipids / Q000032:analysis; D011654:Pulmonary Edema / Q000139:chemically induced / Q000378:metabolism; D011663:Pulmonary Surfactants / Q000008:administration & dosage* / Q000032:analysis / Q000493:pharmacokinetics; D011817:Rabbits; D014018:Tissue Distribution
D009829:Oleic Acids; D010743:Phospholipids; D011663:Pulmonary Surfactants