title: Molecular analysis of zyg-11, a maternal-effect gene required for early embryogenesis of Caenorhabditis elegans.
abstract: The product of the maternally acting gene zyg-11 is required for early embryogenesis of Caenorhabditis elegans. One-cell embryos that lack a functional zyg-11 gene product exhibit an arrest of meiosis at metaphase II, a delay in the formation of pronuclei, unusually vigorous movements of cytoplasm, the formation of multiple pronuclei, incorrect segregation of P granules, and incorrect placement of the first cleavage furrow. We have isolated and sequenced a molecular clone of zyg-11, and shown that microinjection of the cloned DNA can rescue zyg-11 mutations. A transcriptional analysis shows that transcription of the gene is not limited to the female germ-line, despite the strict maternal-effect phenotype of zyg-11 mutations.
authors.affiliation: Section of Genetics and Development, Cornell University, Ithaca, NY 14853.
authors.forename: P W
authors.initials: PW
authors.lastname: Carter
authors.forename: J M
authors.initials: JM
authors.lastname: Roos
authors.forename: K J
authors.initials: KJ
authors.lastname: Kemphues
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002107:Caenorhabditis / Q000196:embryology / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005796:Genes; D008540:Meiosis / Q000235:genetics; D008845:Microinjections; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D015183:Restriction Mapping; D014158:Transcription, Genetic
chemical_list: D004247:DNA