Contrary effect of lactic acid on expression of neuron-specific enolase and glial fibrillary acidic protein in human glioma cells.
We examined the effect of lactic acid on cultured human glioma cell lines expressing glial fibrillary acidic protein (GFAP), vimentin and neuron-specific enolase (NSE). The growth of the cells was inhibited by the lactic acid in a dose-dependent manner. At 56 mM of lactic acid, the surviving cells of the KNS-42-c2 cell line developed slender processes and increasingly formed bizzar giant cells. In an immunofluorescence study of the lactic acid-resistant cells, the GFAP-positive cells prominently decreased in number, while the NSE-positive cells clearly increased. The vimentin was not affected throughout the experiment. After removing lactic acid from the medium, the GFAP-positive cells gradually increased in number. The method of dot immunoassay was useful for quantifying GFAP in cellular extracts. It indicated that the amount of GFAP decreased in the cells cultured with lactate-containing media and increased to the primary values after removing the lactic acid. These results may suggest that the morphological and immunochemical diversities of glioma cells are secondarily affected by cellular microenvironments such as lactic acid.
Takeshita
I
I
Department of Neurosurgery, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Sawa
H
H
Nakamura
T
T
Kuramitsu
M
M
Kitamura
K
K
Fukui
M
M
D002460:Cell Line; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics* / Q000378:metabolism; D005910:Glioma; D006801:Humans; D007773:Lactates / Q000494:pharmacology*; D019344:Lactic Acid; D010751:Phosphopyruvate Hydratase / Q000235:genetics* / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
D005904:Glial Fibrillary Acidic Protein; D007773:Lactates; D014746:Vimentin; D019344:Lactic Acid; D010751:Phosphopyruvate Hydratase