Isolation of hemopoietic precursor cells from human marrow by negative selection using monoclonal antibodies and immunomagnetic beads.
A purified or enriched population of human marrow hemopoietic precursor cells may be produced by using a monoclonal antibody to specifically bind to the desired cell population (positive selection), or by removing undesired cell populations contaminating the mixture (negative selection). We describe a simple negative selection technique using commercially available monoclonal antibodies and immunomagnetic beads to produce a mononuclear marrow cell fraction which comprises no more than 2% of the initial cell population yet contains all the BFU-E and CFU-GM present originally. This procedure is an efficient method for producing hemopoietic precursor cells appropriate for studying cell-substrate adhesions, homing mechanisms, and response to growth factors without interference by contaminating accessory cells.
Cottler-Fox
M
M
Vincent T. Lombardi Cancer Research Center, Georgetown University, Washington, D.C. 20007.
Bazar
L S
LS
Deeg
H J
HJ
D000911:Antibodies, Monoclonal; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D006801:Humans; D008280:Magnetics; D008747:Methylcellulose; D008863:Microspheres; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D008747:Methylcellulose