title: The influence of extracorporeal circulation and hemoseparation on red cell deformability and membrane proteins in coronary artery disease.
abstract: Extracorporeal circulation and hemoseparation may lead to coupled mechanical and chemical blood trauma and thus influence red cell deformability. Ten patients with coronary artery disease underwent coronary bypass. Patients' blood samples were drawn preoperatively, after extracorporeal circulation, and after hemoseparation. Ten healthy adults served as control subjects. Red blood cell deformability was determined by direct microscopic observation of red blood cells subjected to shear stresses of 1.2 to 13.3 Pa with a counter-rotating rheoscope. Red cell membrane proteins were separated by one-dimensional polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. At 1.2 Pa, preoperative red cell deformability was significantly greater in patients with coronary artery disease than in control subjects. Neither extracorporeal circulation nor hemoseparation changed red cell deformability significantly. Electrophoretic separation of membrane proteins failed to show any quantitative or qualitative differences between patients and control subjects. Moreover RBC membrane proteins of red blood cells in the patients were not altered as a result of extracorporeal circulation or hemoseparation. The preoperatively increased red cell deformability in the patients may be drug-induced. Our data suggest that the extracorporeal circulation and hemoseparation techniques used in this study do not lead to red blood cell damage.
authors.affiliation: Department of Pediatrics, University of Heidelberg, Federal Republic of Germany.
authors.forename: R M
authors.initials: RM
authors.lastname: Lohrer
authors.forename: A R
authors.initials: AR
authors.lastname: Trammer
authors.forename: W
authors.initials: W
authors.lastname: Dietrich
authors.forename: S
authors.initials: S
authors.lastname: Hagl
authors.forename: O
authors.initials: O
authors.lastname: Linderkamp
mesh_terms: D001804:Blood Transfusion, Autologous; D002469:Cell Separation; D003327:Coronary Disease / Q000097:blood* / Q000601:surgery; D004907:Erythrocyte Deformability; D004909:Erythrocyte Indices; D005112:Extracorporeal Circulation; D006801:Humans