Actin and myosin expression during development of cardiac muscle from cultured embryonal carcinoma cells.
P19 embryonal carcinoma cells are multipotential stem cells that differentiate into striated muscle as well as some other cell types when aggregated and exposed to dimethyl sulfoxide (DMSO). Immunofluorescence experiments using monospecific antibodies indicated that the majority of muscle cells were mononucleate and contained four myosin isoforms normally found in cardiac muscle; atrial and ventricular myosin heavy chains, ventricular myosin light chain 1, and atrial myosin light chain 2. Northern blot analysis of RNA isolated from differentiating cultures indicated that cardiac actin and skeletal actin mRNAs were expressed at similar levels and with identical kinetics during the differentiation of P19-derived myocytes. These results demonstrate that most of the P19-derived myocytes are of the cardiac type and suggest that they closely resemble the cells of the early embryonic myocardium.
Rudnicki
M A
MA
Department of Medicine, University of Ottawa, Ontario, Canada.
Jackowski
G
G
Saggin
L
L
McBurney
M W
MW
D000199:Actins / Q000032:analysis / Q000235:genetics*; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D051379:Mice; D009132:Muscles / Q000378:metabolism; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism; D009218:Myosins / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D013724:Teratoma
D000199:Actins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D009218:Myosins; D004121:Dimethyl Sulfoxide