title: Studies on membrane lipid peroxidation in omega-3 fatty acid-fed autoimmune mice: effect of vitamin E supplementation.
abstract: Enzyme-dependent and non-enzymatic in vitro lipid peroxidation was studied in autoimmune prone B/W mice fed diets containing high levels of dietary corn oil (CO) or menhaden fish oil (FO) as lipid source since weaning. Lipid analysis revealed that FO-fed mouse liver mitochondrial and microsomal membrane fractions incorporated 20:5 omega 3 and 22:6 omega 3 in replacement of 18:2 omega 6 and 20:4 omega 6 found in corn oil (CO) fed control animals reflecting the composition of the dietary oils. Lower concentrations of vitamin E were found in the FO-fed mouse membranes and serum than those of CO-fed mice when diets were supplemented with a standard 75 I.U. alpha-tocopheryl acetate/kg diet. The rate and extent of membrane lipid peroxidation was greatly increased in FO-fed, vitamin-E-depleted membranes. Full repletion of membrane vitamin E levels by supplementation with 500 I.U./kg of FO diet for 30 days significantly decreased lipid peroxidation and showed that in FO-fed mice, membrane peroxidation is inversely proportional to vitamin E content. However, due to a lower ratio of vitamin E and highly unsaturated fatty acids, FO-fed mouse membranes were more sensitive to pro-oxidant stimulus than were those from CO-fed mice. These findings illustrate the action of vitamin E against membrane lipid peroxidation and stress the importance of adequate supplementation of antioxidant with high omega-3 fatty acids intake.
authors.affiliation: Department of Medicine and Physiology, University of Texas Health Science Center, San Antonio.
authors.forename: S
authors.initials: S
authors.lastname: Laganiere
authors.forename: B P
authors.initials: BP
authors.lastname: Yu
authors.forename: G
authors.initials: G
authors.lastname: Fernandes
mesh_terms: D000818:Animals; D015551:Autoimmunity; D003314:Corn Oil / Q000494:pharmacology; D004041:Dietary Fats / Q000494:pharmacology*; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D005395:Fish Oils / Q000494:pharmacology; D007425:Intracellular Membranes / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008814:Mice, Inbred NZB; D008862:Microsomes, Liver / Q000648:ultrastructure; D008930:Mitochondria, Liver / Q000648:ultrastructure; D024505:Tocopherols; D014810:Vitamin E / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology*; D024502:alpha-Tocopherol / Q000031:analogs & derivatives*
chemical_list: D004041:Dietary Fats; D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D014810:Vitamin E; D003314:Corn Oil; D024502:alpha-Tocopherol; D024505:Tocopherols