Furosemide increases total calcium in kidney and cytoplasmic free calcium in blood mononuclear cells of guinea pigs.
Nephrocalcinosis has been observed in premature infants treated with furosemide. To see whether furosemide-induced renal calcium accumulation is reflected in easily accessible extrarenal cells, we measured cytosolic free calcium ([Ca2+]i) in blood mononuclear cells and kidney tissue calcium of guinea pigs chronically treated with furosemide. At week 0, the mean [Ca2+]i in blood mononuclear cells using the fluorescent indicator quin2 was 105 +/- 4 nM. After 2 weeks of treatment with furosemide, the high dosage (10 or 20 mg/kg/day) caused a significant increase in [Ca2+]i. There was no change in cell volume after 1 or 2 weeks of treatment with furosemide at this dosage. The kidney total calcium concentration and histological calcium accumulation increased with increasing furosemide dosages. These observations suggest that calcium accumulation in blood mononuclear cells may reflect the calcium accumulation in the kidney after furosemide treatment.
Tsao
P W
PW
Division of Cell Biology and Endocrinology, Hospital for Sick Children, Toronto, Canada.
Klip
A
A
Radde
I C
IC
D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D005665:Furosemide / Q000494:pharmacology*; D006168:Guinea Pigs; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D009397:Nephrocalcinosis / Q000503:physiopathology; D011188:Potassium / Q000097:blood; D012964:Sodium / Q000097:blood
D005665:Furosemide; D012964:Sodium; D011188:Potassium; D002118:Calcium