Effect of acute ethanol administration on the subcellular distribution of iron in rat liver and cerebellum.
An acute ethanol load (50 mmol/kg, i.p.) produced altogether a decrease in the non-heme iron content of the serum and an increase in the iron content in liver and cerebellum. Subcellular fractionation studies indicated that the non-heme iron accumulated by the liver, 4 hr after the ethanol load, was recovered in light mitochondria, microsomes and cytosol, and that iron accumulated by the cerebellum was localized in heavy mitochondria, light mitochondria, microsomes and cytosol. The low molecular weight chelatable (LMWC) iron content as well as the percentage of total non-heme iron represented by LMWC-iron were increased in the cytosol of liver and cerebellum after the ethanol load. These results suggest that an acute ethanol load induces (i) a shift in the distribution between circulating and tissular non-heme iron; (ii) an increase in the cytosolic LMWC-iron which, by favouring the biosynthesis of reactive free radicals, may contribute to lipid peroxidation in liver and cerebellum.
Rouach
H
H
Department of Biomedical Research on Alcoholism, Faculté de Médecine, Paris, France.
Houze
P
P
Orfanelli
M T
MT
Gentil
M
M
Bourdon
R
R
Nordmann
R
R
D000818:Animals; D002531:Cerebellum / Q000187:drug effects* / Q000378:metabolism; D003600:Cytosol / Q000187:drug effects; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D007501:Iron / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008861:Microsomes / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains
D000431:Ethanol; D007501:Iron