title: Role of two proline-containing turns in the folding of porcine ribonuclease.
abstract: Unfolded ribonuclease (RNase) from porcine pancreas consists of a mixture of fast and slow-refolding species. The equilibrium distribution of these species differs strongly from other homologous RNases, because an additional proline residue is present at position 115 of the porcine protein. The major slow-folding species of porcine RNase contains incorrect proline isomers at Pro93 and at Pro114-Pro115. Both positions are presumably part of beta-turn structures in the native protein, as deduced from the structure of the homologous bovine RNase A. The folding kinetics of these molecules depend strongly on the conditions used. Under unfavorable conditions (near the unfolding transition), refolding is virtually blocked by the presence of the incorrect proline peptide bonds and partially folded intermediates with incorrect isomers could not be detected. As a consequence, folding is very slow under such conditions and the re-isomerization of Pro114-Pro115 is the first and rate-limiting step of folding. Under strongly native conditions (such as in the presence of ammonium sulfate), refolding is much faster. A largely folded intermediate accumulates with the turns around Pro93 and Pro114-Pro115 still in the non-native conformation. These results suggest that incorrect proline isomers strongly influence protein folding and that, under favorable conditions, the polypeptide chain can fold with two beta-turns locked into a non-native conformation. We conclude, therefore, that early formation of correct turn structure is not necessarily required for protein folding. However, the presence of incorrect turns, locked-in by non-native proline isomers, strongly decreases the rate of refolding. Alternative pathways of folding exist. The choice of pathway depends on the number and distribution of incorrect proline isomers and on the folding conditions.
authors.affiliation: Institut für Biophysik und Physikalische Biochemie Universitaet Regensburg, FRG.
authors.forename: K
authors.initials: K
authors.lastname: Lang
authors.forename: F X
authors.initials: FX
authors.lastname: Schmid
mesh_terms: D000645:Ammonium Sulfate; D000818:Animals; D004795:Enzyme Stability; D019791:Guanidine; D006146:Guanidines; D007700:Kinetics; D011392:Proline; D011487:Protein Conformation; D011489:Protein Denaturation; D012259:Ribonuclease, Pancreatic; D013552:Swine; D013696:Temperature
chemical_list: D006146:Guanidines; D011392:Proline; D012259:Ribonuclease, Pancreatic; D019791:Guanidine; D000645:Ammonium Sulfate