A technique for quantifying the amount of macromolecule injected into cells of the early mouse embryo.
A simple technique for the quantification of the volume injected into an individual mouse egg or blastomere is described. The method does not seem to have deleterious effects on development, allows the progeny of the injected cell to be identified and permits the measurement of the injected volume to be made up to 48 h after the time of injection.
McConnell
J
J
Department of Anatomy, University of Cambridge, UK.
Pickering
S J
SJ
Johnson
M H
MH
Bashford
J
J
D000818:Animals; D004622:Embryo, Mammalian / Q000187:drug effects*; D005260:Female; D046911:Macromolecular Substances; D008722:Methods; D051379:Mice; D011247:Pregnancy; D012333:RNA, Messenger / Q000494:pharmacology*
D046911:Macromolecular Substances; D012333:RNA, Messenger