title: Placental-type alkaline phosphatase in peritoneal fluid of women with endometriosis.
abstract: Alkaline phosphatase (ALP) activities were studied in peritoneal fluid obtained from patients with untreated endometriosis and control women who had normal pelves. The patients (n = 18) had significantly higher total ALP (8.65 +/- 5.69 mU/mg, p less than 0.01) and thermostable ALP (0.28 +/- 0.23 mU/mg, p less than 0.05) than the controls (n = 7) (total ALP = 2.41 +/- 1.15; thermostable ALP = 0.09 +/- 0.04 mU/mg). The contents of the thermostable ALP in the total ALP were 4.5 +/- 2.4% for controls and 3.6 +/- 2.3% for patients. To define the biochemical characteristics of the thermostable ALP, the enzyme was partially purified from a mixture of peritoneal fluid from 16 patients. The partially purified, heated ALP (PFALP) responded to the various inhibitors, activators, and pH values tested in a significantly similar manner as a purified, heated human placental ALP did. Our data suggest that PFALP may be a placental-type (or Regan) ALP.
authors.affiliation: Medical Technology Program, University of Arizona, Tucson 85719.
authors.forename: J O
authors.initials: JO
authors.lastname: Kang
authors.forename: W A
authors.initials: WA
authors.lastname: Hudak
authors.forename: W J
authors.initials: WJ
authors.lastname: Crowley
authors.forename: B S
authors.initials: BS
authors.lastname: Criswell
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis* / Q000037:antagonists & inhibitors; D001202:Ascitic Fluid / Q000201:enzymology*; D004715:Endometriosis / Q000201:enzymology*; D004789:Enzyme Activation; D005260:Female; D058851:GPI-Linked Proteins; D006801:Humans; D007527:Isoenzymes / Q000032:analysis* / Q000037:antagonists & inhibitors; D008274:Magnesium / Q000494:pharmacology; D010386:Pelvic Neoplasms / Q000201:enzymology*; D010920:Placenta / Q000201:enzymology; D015032:Zinc / Q000494:pharmacology
chemical_list: D058851:GPI-Linked Proteins; D007527:Isoenzymes; D000469:Alkaline Phosphatase; C019839:alkaline phosphatase, placental; D008274:Magnesium; D015032:Zinc