Chromatographic determination of dicofol and metabolites in egg yolks.
Egg yolk was spiked with p,p'-dicofol (p,p'-DCF) (0.1-2.0 micrograms/gm), p,p'-dichlorobenzophenone (p,p'-DCBP) (0.1-2.0 micrograms/gm), and 1,1-bis(4-chlorophenyl)-2,2-dichloroethylene (p,p'-DDE) (0.05-1.0 micrograms/gm). The fortified egg yolk (2-5 g) was mixed with acetonitrile to extract non-fat organic materials. After removal of acetonitrile, the spiked chemicals were separated with a column chromatograph packed with acid alumina. Recovery efficiencies for p,p'-DCBP and p,p'-DDE were determined by gas chromatography, and for p,p'-dicofol by high performance liquid chromatography. The recovery efficiencies for p,p'-dicofol, p,p'-DCBP and p,p'-DDE were 77.2-93.8%, 84.1-101.1%, and 88.5-96.0%, respectively.
Mourer
C R
CR
Department of Environmental Toxicology, University of California, Davis 95616.
Hall
G L
GL
Whitehead
W E
WE
Shibamoto
T
T
Shull
L R
LR
Schwarzbach
S E
SE
D000818:Animals; D002645:Chickens; D002849:Chromatography, Gas; D004010:Dicofol / Q000032:analysis* / Q000378:metabolism; D004530:Egg Yolk / Q000032:analysis*; D007306:Insecticides / Q000032:analysis*
D007306:Insecticides; D004010:Dicofol