title: Simultaneous measurement of tetrahydrobiopterin (THBP) and biogenic amines by liquid chromatography with electrochemical detection.
abstract: This report describes a rapid and sensitive method for measuring tetrahydrobiopterin (THBP) and biogenic amines simultaneously by liquid chromatography with electrochemical detection (LC-ECD). The coefficient of variation for THBP was 4.87% and the minimum detectable amount of THBP was approximately 20 pg. These results indicate that this simple reverse-phase ion-pair chromatography system can be used for the simultaneous analysis of endogenous THBP and biogenic amines without long sample preparation time.
authors.affiliation: Laboratory of Experimental Pharmacology, Suntory Institute for Biomedical Research, Osaka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Tani
authors.forename: T
authors.initials: T
authors.lastname: Ishihara
mesh_terms: D000818:Animals; D001679:Biogenic Amines / Q000032:analysis*; D001708:Biopterins / Q000031:analogs & derivatives* / Q000032:analysis; D001923:Brain Chemistry; D002853:Chromatography, Liquid; D004563:Electrochemistry; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D001679:Biogenic Amines; D001708:Biopterins; C003402:sapropterin