Effects of zinc L-carnosine on gastric mucosal and cell damage caused by ethanol in rats. Correlation with endogenous prostaglandin E2.
The effects of zinc L-carnosine on ethanol-induced damage and the correlation of these effects with endogenous prostaglandin E2 were evaluated in rat gastric mucosa in vivo and in vitro. When given either intragastrically or intraperitoneally, zinc L-carnosine (10 or 30 mg/kg) prevented gross visible damage to gastric mucosa caused by ethanol without affecting the mucosal prostaglandin E2 level. This protective effect of zinc L-carnosine was not inhibited by indomethacin. Histological assessment showed that zinc L-carnosine inhibited deep mucosal necrosis, as did 16,16-dimethyl prostaglandin E2. Zinc L-carnosine (10(-6) or 10(-5) M) inhibited the damage caused by ethanol to gastric cells isolated from rat gastric mucosa in vitro; this effect was not inhibited by indomethacin. The results suggested that zinc L-carnosine protects the gastric mucosa and enhances cellular resistance to ethanol without the mediation of endogenous prostaglandins.
Arakawa
T
T
Third Department of Internal Medicine, Osaka City University Medical School, Japan.
Satoh
H
H
Nakamura
A
A
Nebiki
H
H
Fukuda
T
T
Sakuma
H
H
Nakamura
H
H
Ishikawa
M
M
Seiki
M
M
Kobayashi
K
K
D015064:16,16-Dimethylprostaglandin E2 / Q000494:pharmacology; D000818:Animals; D000897:Anti-Ulcer Agents / Q000627:therapeutic use*; D002336:Carnosine / Q000627:therapeutic use*; D015232:Dinoprostone / Q000378:metabolism*; D004151:Dipeptides / Q000627:therapeutic use*; D000431:Ethanol / Q000037:antagonists & inhibitors / Q000633:toxicity*; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007213:Indomethacin / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013276:Stomach Ulcer / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D015032:Zinc / Q000627:therapeutic use*
D000897:Anti-Ulcer Agents; D004151:Dipeptides; D000431:Ethanol; D002336:Carnosine; D015032:Zinc; D015232:Dinoprostone; D015064:16,16-Dimethylprostaglandin E2; D007213:Indomethacin