Characteristics and localisation of 125I ion binding in mammalian airways.
We have examined some of the binding characteristics and the autoradiographic distribution of binding sites for Na125I (I-Na) in airway tissue from the guinea-pig, monkey, pig, rat, mouse and from man. Basal I-Na (100 pM) binding levels were extremely low. However, in the presence of ascorbic acid (10 microM) or dithiothreitol (10 microM), I-Na binding was markedly increased in guinea-pig trachea, with lesser increases detected in monkey and rat trachea and in monkey and human bronchus. In guinea-pig trachea, ascorbic acid-induced I-Na binding was not saturable within the concentration range 100-620 pM and could not be reduced by washout. Autoradiography revealed that in central airways, I-Na binding was localized at or near the interface of the airway epithelium and submucosa in small clusters, apparently involving one or two cells per focus. The physiological significance of these binding sites is yet to be established, although they may be involved in intracellular iodine storage.
Self
G J
GJ
Department of Pharmacology, University of Western Australia, Perth, Nedlands.
Rigby
P J
PJ
Passarelli
M C
MC
Goldie
R G
RG
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D006168:Guinea Pigs; D006801:Humans; D066298:In Vitro Techniques; D007454:Iodides / Q000493:pharmacokinetics*; D007457:Iodine Radioisotopes; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D008252:Macaca fascicularis; D051379:Mice; D008808:Mice, Inbred CBA; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism*; D010869:Pindolol / Q000031:analogs & derivatives; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D012974:Sodium Iodide / Q000493:pharmacokinetics*; D013045:Species Specificity; D013552:Swine; D014132:Trachea / Q000187:drug effects / Q000378:metabolism
D007454:Iodides; D007457:Iodine Radioisotopes; C047975:cyanopindolol; D010869:Pindolol; D012974:Sodium Iodide; D001205:Ascorbic Acid; D004229:Dithiothreitol