title: Preliminary crystallographic study of phospholipase A2 from the venom of Trimeresurus flavoviridis (habu snake).
abstract: Crystallization and a preliminary crystallographic study of Trimeresurus flavoviridis (habu snake) phospholipase A2 (PLA2) were carried out. Although crystals were obtained from various solutions, crystals suitable for X-ray analysis could be obtained from polyethylene glycol solutions only when a repeated seeding technique was applied starting from twinned crystals. The crystal is monoclinic with space group P21, with a = 44.1, b = 55.7, c = 48.8 A, and beta = 92.4 degrees. An asymmetric unit contains a dimer consisting of two identical subunits made of 122 amino acids. The crystal reflects X-rays beyond 2.5 A. A Pt derivative gave a good isomorphous crystal.
authors.affiliation: Department of Applied Chemistry, Faculty of Engineering, Nagoya University, Aichi.
authors.forename: Y
authors.initials: Y
authors.lastname: Shinoda
authors.forename: A
authors.initials: A
authors.lastname: Shouji
authors.forename: T
authors.initials: T
authors.lastname: Yamane
authors.forename: A
authors.initials: A
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Ashida
authors.forename: H
authors.initials: H
authors.lastname: Kihara
authors.forename: M
authors.initials: M
authors.lastname: Ohno
mesh_terms: D000818:Animals; D003435:Crotalid Venoms; D003460:Crystallization; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D011487:Protein Conformation; D014961:X-Ray Diffraction
chemical_list: D003435:Crotalid Venoms; C029748:Trimeresurus venoms; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2