Inhibition of alpha-aminoisobutyric acid uptake by mastoparan in Madin-Darby Canine Kidney cells.
Mastoparan, a wasp venom, was found to inhibit Na(+)-dependent net alpha-aminoisobutyric acid (AIB) uptake in Madin Darby Canine Kidney (MDCK) cells. Mastoparan also produced a significant increase in AIB efflux when compared to controls. Pretreatment of MDCK cells with 2 mM neomycin attenuated mastoparan's inhibition of net AIB uptake and completely suppressed mastoparan-mediated increases in AIB efflux when compared to controls. These data suggest that mastoparan's inhibition of net AIB uptake involves more than a single basic mechanism.
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Department of Physiology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814-4799.
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D000621:Aminoisobutyric Acids / Q000378:metabolism*; D000818:Animals; D001514:Bee Venoms / Q000494:pharmacology*; D001693:Biological Transport, Active / Q000187:drug effects; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D036341:Intercellular Signaling Peptides and Proteins; D007700:Kinetics; D009355:Neomycin / Q000494:pharmacology; D010042:Ouabain / Q000494:pharmacology; D010455:Peptides; D012964:Sodium / Q000494:pharmacology; D014862:Wasp Venoms / Q000494:pharmacology*
D000621:Aminoisobutyric Acids; D001514:Bee Venoms; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D014862:Wasp Venoms; D010042:Ouabain; C037500:mastoparan; D012964:Sodium; D009355:Neomycin