title: 5'-Nucleotidase is involved in chick embryo myoblast spreading on laminin.
abstract: Previous studies have shown that 5'-nucleotidase, an ectoenzyme from chicken gizzard, interacts specifically with laminin and fibronectin, two glycoproteins of the extracellular matrix. Recently, we demonstrated that 5'-nucleotidase was involved in the spreading of chick embryo fibroblast on laminin. In the present communication, we report that a monoclonal antibody (CG37) raised-directed against 5'-nucleotidase inhibited the spreading of chick embryo myoblasts on laminin after their initial attachment to the substrate. Furthermore, monoclonal antibody CG37 specifically eluted 5'-nucleotidase from immobilized laminin and thus enabled its isolation from other myoblast laminin-binding proteins.
authors.affiliation: Unité 180 INSERM, Laboratoire de Biologie et Pathologie Moléculaires des Glycoprotéines, Paris, France.
authors.forename: B
authors.initials: B
authors.lastname: Mehul
authors.forename: M A
authors.initials: MA
authors.lastname: Doyennette-Moyne
authors.forename: M
authors.initials: M
authors.lastname: Aubery
authors.forename: H G
authors.initials: HG
authors.lastname: Mannherz
authors.forename: P
authors.initials: P
authors.lastname: Codogno
mesh_terms: D015720:5'-Nucleotidase / Q000302:isolation & purification / Q000502:physiology*; D000818:Animals; D000911:Antibodies, Monoclonal; D002448:Cell Adhesion / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D002478:Cells, Cultured; D002642:Chick Embryo; D002846:Chromatography, Affinity; D007797:Laminin; D009132:Muscles / Q000166:cytology* / Q000196:embryology
chemical_list: D000911:Antibodies, Monoclonal; D007797:Laminin; D015720:5'-Nucleotidase