Growth of Corynebacterium bovis in mammary secretions during physiological transitions of the bovine mammary gland.
An in vitro microassay was used to evaluate growth of five strains of Corynebacterium bovis in mammary secretions collected from quarters of five Holstein cows at 0, 14, and 28 d of involution, at parturition, and 14 d after parturition. Variation in growth among different strains of Corynebacterium bovis was observed. Corynebacterium bovis grew well in mammary secretions obtained at the last milking of lactation, at parturition, and 14 d after parturition. However, growth of four strains of Corynebacterium bovis in mammary secretions obtained at 14 and 28 d of involution was reduced significantly. In contrast, a streptomycin-resistant strain of Corynebacterium bovis grew well in mammary secretions obtained during involution. These data suggest that mammary secretions support growth of Corynebacterium bovis during lactation but inhibit growth during the nonlactating period. Inhibition of growth in secretions collected during the nonlactating period may be associated with the high rate of spontaneous elimination of Corynebacterium bovis intramammary infection from cessation of milking to parturition. Conversely, enhanced growth in milk may be related to persistent Corynebacterium bovis intramammary infections during lactation.
Oliver
S P
SP
Department of Animal Science, University of Tennessee, Knoxville 37901-1071.
Juneja
V K
VK
D000818:Animals; D002417:Cattle; D003352:Corynebacterium / Q000254:growth & development*; D003354:Corynebacterium Infections / Q000209:etiology / Q000662:veterinary; D005260:Female; D007743:Labor, Obstetric / Q000502:physiology*; D007774:Lactation / Q000502:physiology*; D008321:Mammary Glands, Animal / Q000378:metabolism / Q000382:microbiology*; D008414:Mastitis, Bovine / Q000209:etiology; D008892:Milk / Q000382:microbiology*; D011247:Pregnancy