title: DNA ploidy and S phase fraction in human bladder cancer. Relation to survival and histological grade (WHO).
abstract: The nuclear DNA content of archival paraffin-embedded bladder cancer samples (70 patients) of WHO grades I-III has been measured by flow cytometry. The female/male ratio was 15/55. The mean follow-up time was 13 years (range 9.6-22.0 years). 37 of 70 (53%) patients had DNA index 1.0 (diploid DNA content), and the remaining 33 (47%) patients had an aneuploid tumor. There was no significant difference in the age (mean +/- SD) of the patients having a diploid (66 +/- 9 years) or an aneuploid tumor (68 +/- 11 years) at the time of diagnosis. 47 deaths occurred during the follow-up period; 24 (51%) of these were due to bladder cancer (12 diploid, 12 aneuploid tumors). No significant difference was found after radical treatment during the disease-free interval (mean +/- SD) between diploid (48 +/- 45 months) and aneuploid (35.5 +/- 35 months) groups of patients. Recurrences during the follow-up period were equally common among aneuploid and diploid tumors. A statistically significant relation between histological grade and survival could be demonstrated, but DNA ploidy and S phase fraction had little prognostic value in this respect. There was no statistically significant difference in survival between aneuploid (30%) and diploid (35%) groups of tumors during the follow-up period. The study suggests that flow cytometric determination of nuclear DNA ploidy from paraffin-embedded samples in bladder tumors does not add to the prognostic power of subjective histological grading.
authors.affiliation: Department of Pathology, University of Kuopio, Finland.
authors.forename: P K
authors.initials: PK
authors.lastname: Lipponen
authors.forename: M J
authors.initials: MJ
authors.lastname: Eskelinen
authors.forename: Y
authors.initials: Y
authors.lastname: Collan
authors.forename: E
authors.initials: E
authors.lastname: Pesonen
authors.forename: T
authors.initials: T
authors.lastname: Ventilä
authors.forename: M
authors.initials: M
authors.lastname: Sotarauta
authors.forename: S
authors.initials: S
authors.lastname: Nordling
mesh_terms: D000368:Aged; D002295:Carcinoma, Transitional Cell / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D004273:DNA, Neoplasm / Q000648:ultrastructure*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007399:Interphase; D008297:Male; D011003:Ploidies; D011379:Prognosis; D015996:Survival Rate; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology
chemical_list: D004273:DNA, Neoplasm