title: Electron microscopy mapping of pBR322 DNA curvature. Comparison with theoretical models.
abstract: A map of local curvature of the pBR322 DNA has been established by electron microscopy analysis of linearized plasmid molecules. To determine their polarity these molecules are one end labelled with an avidin-ferritin-biotin complex and the images are digitized. Local curvature is calculated from two mathematical treatments of the DNA trajectory and expressed in term of a mean dinucleotide wedge angle. Eight regions of curvature are distinguished. The four main regions of curvature have a high content of phased AA runs. The experimental curvature map is compared to theoretical maps of curvature obtained from four available models for DNA curvature.
authors.affiliation: Laboratoire de Pharmacologie Moléculaire, (CNRS UA 147) Institut Gustave-Roussy, Villejuif, France.
authors.forename: G
authors.initials: G
authors.lastname: Muzard
authors.forename: B
authors.initials: B
authors.lastname: Théveny
authors.forename: B
authors.initials: B
authors.lastname: Révet
mesh_terms: D001482:Base Composition; D004269:DNA, Bacterial / Q000648:ultrastructure*; D004278:DNA, Superhelical / Q000648:ultrastructure*; D008433:Mathematics; D008854:Microscopy, Electron / Q000379:methods; D008962:Models, Theoretical; D009690:Nucleic Acid Conformation; D010957:Plasmids; D015183:Restriction Mapping
chemical_list: D004269:DNA, Bacterial; D004278:DNA, Superhelical