title: Peptide substrate specificity of the alpha-amidating enzyme isolated from rat medullary thyroid CA-77 cells.
abstract: The kinetic parameters were obtained for enzymatic alpha-amidation of peptides of the form N-dansyl-(Gly)4-X-Gly-OH, in which the amino acid at position X was substituted with each of the 20 natural amino acids. The enzyme used in these studies was a highly enriched preparation of alpha-amidating enzyme secreted by a clonal (CA-77) cell line which actively expresses mature alpha-amidated peptides. A 130-fold and 11-fold variation respectively in apparent Km and Vmax values was observed. The effect of the amino acid side chain at position X in stabilization of the enzyme-substrate complex decreased through the series X = planar aromatic or sulfur containing greater than neutral aliphatic greater than polar and basic greater than cyclic aliphatic or acidic.
authors.affiliation: Unigene Laboratories, Inc., Fairfield, New Jersey.
authors.forename: P P
authors.initials: PP
authors.lastname: Tamburini
authors.forename: S D
authors.initials: SD
authors.lastname: Young
authors.forename: B N
authors.initials: BN
authors.lastname: Jones
authors.forename: R A
authors.initials: RA
authors.lastname: Palmesino
authors.forename: A P
authors.initials: AP
authors.lastname: Consalvo
mesh_terms: D000818:Animals; D007700:Kinetics; D006899:Mixed Function Oxygenases; D009097:Multienzyme Complexes; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000378:metabolism*; D051381:Rats; D013379:Substrate Specificity; D013964:Thyroid Neoplasms / Q000201:enzymology*; D014407:Tumor Cells, Cultured
chemical_list: D009097:Multienzyme Complexes; D006899:Mixed Function Oxygenases; C035189:peptidylglycine monooxygenase; D000587:Oxidoreductases Acting on CH-NH Group Donors