Use of free and transthyretin-bound retinol-binding protein in serum as tests of vitamin A status in humans: effect of high creatinine concentrations in serum.
We measured immunologically active (apo + holo) retinol-binding protein (RBP), vitamin A-carrying (holo) free RBP, and transthyretin-bound (TTR) holo-RBP in serum from 34 retrospective cases of fluctuating acute renal failure. All subjects had high serum creatinine concentrations caused by renal failure. Apo + holo, holo-TTR-RBP, and (especially) holo-free RBP all correlated poorly but significantly with serum creatinine concentration. Therefore, the use of any form of RBP to measure vitamin A status may be of limited value in subjects with high creatinine concentrations in serum. However, molecular-exclusion HPLC may be able to distinguish increases in RBP concentration associated with renal failure from those caused by altered vitamin A status, because renal failure causes abnormalities in the number and retention times of chromatographic peaks as well as their areas.
Burri
B J
BJ
Western Human Nutrition Research Center, USDA/ARS/PWA, Presidio of San Francisco, CA 94129.
Bankson
D D
DD
Neidlinger
T R
TR
D058186:Acute Kidney Injury / Q000097:blood; D000328:Adult; D002851:Chromatography, High Pressure Liquid; D003404:Creatinine / Q000097:blood*; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D008297:Male; D009752:Nutritional Status; D011228:Prealbumin / Q000032:analysis* / Q000378:metabolism; D012177:Retinol-Binding Proteins / Q000032:analysis* / Q000378:metabolism; D014801:Vitamin A / Q000097:blood* / Q000378:metabolism
D011228:Prealbumin; D012177:Retinol-Binding Proteins; D014801:Vitamin A; D003404:Creatinine