title: N-methylmitomycin A cross-linking to nucleosomal structure.
abstract: The cross-linking reaction of N-methylmitomycin A to 175-bp calf thymus nucleosomes and to reconstituted core particles having known DNA sequences was examined using gel-electrophoresis techniques. The nucleosome structure, as the CAP-DNA complex, leads to a decreased covalent binding in comparison to protein-free DNA, suggesting, in addition to sequence specificity, precise geometrical requirements for bifunctional covalent addition of the drug to the nucleic acid.
authors.affiliation: Department of Organic Chemistry, University of Padua, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Cera
authors.forename: M
authors.initials: M
authors.lastname: Palumbo
authors.forename: G
authors.initials: G
authors.lastname: Palu
authors.forename: D M
authors.initials: DM
authors.lastname: Crothers
mesh_terms: D000818:Animals; D001482:Base Composition / Q000187:drug effects / Q000502:physiology; D002417:Cattle; D003432:Cross-Linking Reagents; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D004347:Drug Interactions; D004591:Electrophoresis, Polyacrylamide Gel; D008937:Mitomycins / Q000032:analysis / Q000493:pharmacokinetics*; D008969:Molecular Sequence Data; D009707:Nucleosomes / Q000187:drug effects* / Q000378:metabolism; D013950:Thymus Gland / Q000187:drug effects / Q000378:metabolism
chemical_list: D003432:Cross-Linking Reagents; D008937:Mitomycins; D009707:Nucleosomes; D004247:DNA; C014675:N-methylmitomycin A