Analysis of the 2-keto-3-deoxyoctonate (KDO) region of lipopolysaccharides isolated from non-01 Vibrio cholerae 05R.
Phosphorylated 2-keto-3-deoxyoctonate (KDO) has been detected in the strong-acid hydrolysates of lipopolysaccharides (LPS) of family Vibrionaceae including Vibrio cholerae. Structural analysis of LPS isolated from a rough mutant of non-01 V. cholerae 05 by dephosphorylation, periodate oxidation and methylation analysis revealed that the inner core region of the LPS molecule contains only one mole of KDO in contrast to enteric Gram-negative bacterial LPS, and that the phosphate group on the KDO molecule resides in the C4 position, while the site of binding of KDO to heptose, a constituent of the distal part of the inner core region, is the C5 position as in the enteric bacterial LPS.
Kondo
S
S
Department of Microbiology, School of Pharmaceutical Sciences Josai University, Saitama, Japan.
Haishima
Y
Y
Hisatsune
K
K
D002849:Chromatography, Gas; D004590:Electrophoresis, Paper; D008401:Gas Chromatography-Mass Spectrometry; D008070:Lipopolysaccharides / Q000302:isolation & purification; D008745:Methylation; D015394:Molecular Structure; D010766:Phosphorylation; D013400:Sugar Acids / Q000032:analysis*; D014734:Vibrio cholerae / Q000032:analysis*
D008070:Lipopolysaccharides; D013400:Sugar Acids; C002532:2-keto-3-deoxyoctonate