Characterization of DNA pattern in the site of permanent attachment to the nuclear matrix located in the vicinity of replication origin.
The permanent sites of DNA attachment to the nuclear matrix in the domain of chicken alpha-globin genes originally found in erythrocyte nuclei have also been shown to exist in sperm and cultured fibroblast cells. A primary structure of a 1.7 kb fragment located in 5'-upstream region of chicken alpha-globin gene domain and containing both replication origin and permanent nuclear matrix attachment site has been determined. It was found to possess homologies with papovaviral replication origins and contain short internal repeats and GC-rich motifs.
Kalandadze
A G
AG
Engelhardt Institute of Molecular Biology, Academy of Sciences of the USSR, Moscow.
Bushara
S A
SA
Vassetzky
Y S
YS
Razin
S V
SV
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D015139:Blotting, Southern; D002467:Cell Nucleus / Q000502:physiology* / Q000648:ultrastructure; D002645:Chickens; D004261:DNA Replication; D003852:Deoxyribonucleoproteins / Q000648:ultrastructure; D005796:Genes; D005914:Globins / Q000235:genetics*; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D015530:Nuclear Matrix / Q000648:ultrastructure; D009687:Nuclear Proteins / Q000378:metabolism; D009690:Nucleic Acid Conformation; D012045:Regulatory Sequences, Nucleic Acid
D003852:Deoxyribonucleoproteins; D009687:Nuclear Proteins; D005914:Globins