title: Production, characterization, and species specificity of monoclonal antibodies to Mycobacterium avium complex protein antigens.
abstract: The incidence of Mycobacterium avium-Mycobacterium intracellulare complex infections has increased in recent years primarily because a significant proportion of acquired immunodeficiency syndrome patients develop disseminated M. avium complex disease. In an effort to develop new tools to study these infections, we have produced eight monoclonal antibodies directed against M. avium. Western blot (immunoblot) specificity analysis and protease sensitivity assays indicate that four of these antibodies recognize M. avium-specific protein epitopes and two react with M. avium complex-specific peptide determinants. These monoclonal antibodies may be useful clinically in the diagnosis of M. avium complex disease and in the laboratory for isolation and characterization of native and recombinant M. avium complex antigens.
authors.affiliation: Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892.
authors.forename: D A
authors.initials: DA
authors.lastname: Rouse
authors.forename: S L
authors.initials: SL
authors.lastname: Morris
authors.forename: A B
authors.initials: AB
authors.lastname: Karpas
authors.forename: P G
authors.initials: PG
authors.lastname: Probst
authors.forename: S D
authors.initials: SD
authors.lastname: Chaparas
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology*; D015153:Blotting, Western; D003429:Cross Reactions; D006801:Humans; D007132:Immunoglobulin Isotypes / Q000032:analysis; D008970:Molecular Weight; D015269:Mycobacterium avium Complex / Q000276:immunology*; D013045:Species Specificity
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D007132:Immunoglobulin Isotypes