title: Electrophysiological properties of rat septal region neurons during development in culture.
abstract: We report on the development of membrane properties of septal region neurons from embryonic rats in serum-free culture during 1-25 days in vitro (DIV). Na(+)-dependent action potentials could be evoked within 1 day after plating and 3 different types of outward current were observed by means of the patch-clamp technique: IK, IA and IC. In some neurons the neurotransmitter GABA evoked a chloride current after 2 DIV. In addition a cationic current elicited by glutamate appeared after 4 DIV. Within 8-12 DIV virtually all neurons were sensitive to both GABA and glutamate. Spontaneous action potentials and postsynaptic potentials occurred after 7-10 DIV but cultured septal neurons did not generate any pacemaker-like activity.
authors.affiliation: Department of Neurology, University of Düsseldorf, F.R.G.
authors.forename: H
authors.initials: H
authors.lastname: Köller
authors.forename: M
authors.initials: M
authors.lastname: Siebler
authors.forename: C
authors.initials: C
authors.lastname: Schmalenbach
authors.forename: H W
authors.initials: HW
authors.lastname: Müller
mesh_terms: D000200:Action Potentials; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D008564:Membrane Potentials; D051381:Rats; D011919:Rats, Inbred Strains; D012686:Septal Nuclei / Q000166:cytology / Q000502:physiology*; D013779:Tetrodotoxin / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
chemical_list: D013779:Tetrodotoxin; D005680:gamma-Aminobutyric Acid