Inhibition of MEL cells' capacity to undergo erythroid differentiation by chemicals added during induction.
Erythroid differentiation of murine erythroleukemia (MEL) cells, as induced by dimethyl sulfoxide, can be suppressed by chemicals at very low concentrations, not affecting cell viability and proliferation, if present in the culture medium between 18 and 24 h after addition of the inducer. The effect is apparent on the progeny of the treated cells and is determined, between day 3 and 5 following DMSO induction, as percent value of cells expressing the erythroid phenotype. Cultures showing decreased values are no longer terminal and a large number of clones, incapable of expressing the erythroid phenotype, can be isolated from them. In contrast, induced cultures are terminal if the added chemicals do not decrease the expression of the erythroid phenotype. Incorporation of thymidine into induced cultures reveals that maximal sensitivity of MEL cells to chemicals coincides with DNA duplication. In all affected cells, the inhibition to undergo erythroid differentiation is transmitted from one cell generation to the next.
Foresti
M
M
Dipartimento di Genetica, Università di Napoli, Italy.
Gaudio
L
L
Geraci
G
G
D002454:Cell Differentiation / Q000187:drug effects; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D004121:Dimethyl Sulfoxide; D015672:Erythroid Precursor Cells / Q000187:drug effects*; D007399:Interphase; D008745:Methylation; D009153:Mutagens / Q000494:pharmacology*; D010641:Phenotype; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured
D009153:Mutagens; D004247:DNA; D004121:Dimethyl Sulfoxide