Radiolocalization of human prostate tumor in a mouse subrenal capsule model by monoclonal antibody TURP-27.
The subrenal capsule assay was used to determine if 125I-labeled anti-prostate monoclonal antibody TURP-27 could target human prostate tumor fragments implanted under the renal capsule of normal immunocompetent C57 BL/6 mice. Maximal binding and optimal tumor to non-tumor tissue ratios occurred within 24-48 hours postadministration of 125I-TURP-27. No significant localization was observed in mice bearing TURP-27 antigen-negative human colon tumor tissue implants or with an isotype-matched control monoclonal antibody. These preclinical data suggest that TURP-27 may have clinical application for imaging metastatic prostate tumors and further application in immunoconjugate and/or radiotherapy of prostate cancer.
Wright
G L
GL
Department of Microbiology and Immunology, Eastern Virginia Medical School, Norfolk 23501.
Wilson
E
E
Klinger
M
M
Roland
W
W
Beckett
M L
ML
Daddona
P E
PE
D000818:Animals; D000911:Antibodies, Monoclonal; D006801:Humans; D007457:Iodine Radioisotopes; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011467:Prostate / Q000276:immunology; D011471:Prostatic Neoplasms / Q000175:diagnosis*; D013370:Subrenal Capsule Assay
D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes