NMR spectral analysis of kinetic data using natural lineshapes.
An automated rapid data analysis scheme for NMR spectroscopy time course studies is presented. This method uses a high signal-to-noise reference spectrum collected at the beginning of a time course as a lineshape model to analyze subsequent low signal-to-noise data collected with higher time resolution. The method is fast (approximately 1 s to evaluate two peaks in a 2K spectrum) and easily implemented on NMR spectrometer computers. An application of this method to spectroscopic studies on the heart is provided.
Heineman
F W
FW
Laboratory of Cardiac Energetics, National Heart Blood and Lung Institute, Bethesda, Maryland 20892.
Eng
J
J
Berkowitz
B A
BA
Balaban
R S
RS
D006801:Humans; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008961:Models, Structural; D013997:Time Factors