The autoradiographic localization of adenylate cyclase in rat kidney using [3H]forskolin.
The localization of [3H]forskolin binding to microscope slide mounted sections of rat kidney has been examined using autoradiography. Saturation studies showed [3H]forskolin binding to two sites, a high affinity site (KD = 8.7 nM, Bmax = 0.14 pmol/mg protein) and a low affinity site (KD = 6.7 microM, Bmax = 11.0 pmol/mg protein). Autoradiographs showed high affinity binding (thought to identify stimulatory guanine nucleotide binding protein (Gs)-linked adenylate cyclase) to all renal structures known to possess hormone sensitive adenylate cyclase, including all tubular segments, glomeruli and blood vessels. High concentrations of binding were associated with a portion of the proximal tubule and with papillary collecting tubules and ducts.
McMartin
L R
LR
Department of Pharmacology, University of Melbourne, Parkville, Australia.
Summers
R J
RJ
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography; D001665:Binding Sites; D005576:Colforsin / Q000378:metabolism; D005260:Female; D007668:Kidney / Q000201:enzymology* / Q000378:metabolism; D007672:Kidney Cortex / Q000201:enzymology; D007684:Kidney Tubules / Q000201:enzymology; D007700:Kinetics; D011869:Radioligand Assay; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium
D014316:Tritium; D005576:Colforsin; D000262:Adenylyl Cyclases