title: Depolarization of the neuronal membrane caused by cotransport of taurine and sodium.
abstract: C 1300 neuroblastoma cells were cultured and used to study the effect of sodium dependent taurine transport on the membrane potential. Measuring net accumulation of taurine and the depolarization caused by externally applied taurine, we found both processes become active at an external concentration of taurine of 1 mM or more. Net accumulation had Km of 13 mM and a Vmax of 126 nmol x mg of protein-1 x min-1. The taurine induced depolarization of the neuroblastoma cell was parallelled by a 25 per cent decrease in its membrane impedance. The transport of taurine, the depolarization caused by taurine and the effect of taurine on the membrane impedance, all, had a similar dependence on the external sodium concentration. Our results on the depolarizing cotransport between taurine and sodium at the neuronal membrane, may illustrate an additional mechanism for the control of the electrical activity of neuronal cells.
authors.affiliation: Department of Biomedical Sciences, University of Tampere, Finland.
authors.forename: I
authors.initials: I
authors.lastname: Holopainen
authors.forename: E
authors.initials: E
authors.lastname: Lidén
authors.forename: A
authors.initials: A
authors.lastname: Nilsson
authors.forename: A
authors.initials: A
authors.lastname: Sellström
mesh_terms: D002460:Cell Line; D006801:Humans; D009447:Neuroblastoma; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D012964:Sodium / Q000493:pharmacokinetics* / Q000502:physiology; D013654:Taurine / Q000378:metabolism / Q000493:pharmacokinetics*
chemical_list: D013654:Taurine; D012964:Sodium