Evaluation of photometric high-performance liquid chromatographic data for the determination of benflurone metabolites in biological materials with and without concomitant use of a standard.
The results produced by a new method of calculation based on the knowledge of the absorption coefficient and instrumental parameters without the concomitant use of a standard were compared with those calculated by the routine external standard method for a model system utilizing the quantification of benzofluorene derivatives. These were present in the incubation mixture of 5-[2-(dimethylamino)ethoxy]-7-oxo-7H-benzo[c]fluorene (benflurone) with the microsomal fraction of rat liver homogenate. Reasonable agreement between the two methods was observed. The potential utility of the new method of calculation is discussed.
Hais
I M
IM
Institute of Experimental Biopharmacy, Czechoslovak Academy of Sciences, Hradec Králové.
Nobilis
M
M
Kvasnicková
E
E
D000818:Animals; D000970:Antineoplastic Agents / Q000032:analysis* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005069:Evaluation Studies as Topic; D005449:Fluorenes / Q000032:analysis* / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism; D011817:Rabbits
D000970:Antineoplastic Agents; D005449:Fluorenes; C034234:VUFB 13468