title: Haptoglobin subtype determination by isoelectric focusing in agarose gel: application to paternity testing and presentation of a new alpha 2-variant.
abstract: Haptoglobin subtyping of a Danish population sample (n = 2184) by a modified isoelectric focusing/immunoblotting method, using agarose gel and visualization by an alkaline phosphatase conjugated antibody, is presented. The allele frequencies were: Hp*1F 0.151; Hp*1S 0.241; Hp*2FS 0.565; Hp*2SS 0.040; Hp*2FF 0.002; Hp J*0.0002. Based on these data the theoretical change of exclusion of nonfathers in paternity cases was calculated to be 35%. Examination of 51 families with 120 children and of 648 mother/child pairs showed no exceptions from Mendelian inheritance. The results obtained by application of Hp subtyping to 405 paternity cases are given and the reliability of the method is discussed. A new alpha 2-variant occurring in the father and in two of three siblings of a Danish family is presented.
authors.affiliation: Institute of Forensic Genetics, University of Copenhagen, Denmark.
authors.forename: M
authors.initials: M
authors.lastname: Thymann
authors.forename: O
authors.initials: O
authors.lastname: Svensmark
authors.forename: G
authors.initials: G
authors.lastname: Masumba
authors.forename: H
authors.initials: H
authors.lastname: Broksø
authors.forename: L B
authors.initials: LB
authors.lastname: Skibsby
mesh_terms: D000483:Alleles; D001793:Blood Preservation; D003718:Denmark; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation; D006242:Haptoglobins / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D015151:Immunoblotting; D007525:Isoelectric Focusing; D008297:Male; D010334:Paternity; D010641:Phenotype
chemical_list: D006242:Haptoglobins