Cerebral metabolite dynamics during temporary complete ischemia in rats monitored by time-shared 1H and 31P NMR spectroscopy.
The changes in cerebral phosphorus metabolites, intracellular pH, and lactate during 30 min of complete global ischemia and 2 h of reperfusion were monitored by time-shared 1H and 31P in vivo NMR spectroscopy in rats. After the induction of ischemia, intracellular pH decreased from 7.14 +/- 0.01 to 6.32 +/- 0.10, and lactate concentration increased from 1.6 +/- 0.4 to 15.8 +/- 2.5 mumol/g; ATP and phosphocreatine were totally depleted, while inorganic phosphate increased 715 +/- 47%. Within 1 h after blood flow was restored, high-energy phosphates and lactate levels had recovered close to baseline levels. The changes in intracellular pH and lactate levels during ischemia and reperfusion correlated well.
Chang
L H
LH
Department of Pharmaceutical Chemistry, School of Medicine, University of California, San Francisco 94143.
Shirane
R
R
Weinstein
P R
PR
James
T L
TL
D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D002545:Brain Ischemia / Q000378:metabolism*; D006859:Hydrogen; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D010725:Phosphocreatine / Q000032:analysis; D010758:Phosphorus; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D007773:Lactates; D010725:Phosphocreatine; D010758:Phosphorus; D006859:Hydrogen; D000255:Adenosine Triphosphate