Transforming growth factor-beta regulates the splicing pattern of fibronectin messenger RNA precursor.
Fibronectin (FN) polymorphism is caused by alternative splicing patterns in at least three regions (ED-A, ED-B and IIICS) of the primary transcript of a single gene. Using monoclonal antibodies, we previously demonstrated that transforming growth factor-beta (TGF-beta) preferentially increases the accumulation of the FN isoforms containing the ED-A sequence in cultured normal human fibroblasts [Balza et al., (1988) FEBS Lett. 228, 42-44]. To determine the basis of this effect, we have examined through S1 nuclease analysis, the levels of ED-A- and ED-B-containing mRNAs in cultured normal human skin fibroblasts before and after TGF-beta treatment. These experiments have shown that TGF-beta increases the relative amount of m-RNA for ED-A- and ED-B-containing FN isoforms. These data demonstrate that a growth factor may regulate the splicing pattern of a pre-mRNA.
Borsi
L
L
Cell Biology Laboratory, Instituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
Castellani
P
P
Risso
A M
AM
Leprini
A
A
Zardi
L
L
D002460:Cell Line; D015342:DNA Probes; D005091:Exons; D005347:Fibroblasts / Q000378:metabolism; D005353:Fibronectins / Q000235:genetics*; D006801:Humans; D008233:Lymphotoxin-alpha / Q000494:pharmacology*; D009693:Nucleic Acid Hybridization; D012322:RNA Precursors / Q000235:genetics*; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*; D015719:Single-Strand Specific DNA and RNA Endonucleases
D015342:DNA Probes; D005353:Fibronectins; D008233:Lymphotoxin-alpha; D012322:RNA Precursors; D012333:RNA, Messenger; D015719:Single-Strand Specific DNA and RNA Endonucleases