Chemical synthesis of bovine transforming growth factor-alpha: synthesis, characterization and biological activity.
Bovine transforming growth factor-alpha (bTGF-alpha) is a 50 amino acid polypeptide with three disulfide linkages. In order to evaluate the biological function of this peptide, bTGF-alpha was synthesized via an automatic synthesizer and purified to homogeneity in high yield. The integrity of this synthetic peptide was confirmed by chemical analyses and bioassays. In a bovine liver radioreceptor assay, bTGF-alpha competes with radiolabeled EGF and has activity comparable to mEGF and hTGF-alpha. Compared to hEGF, bTGF-alpha elicits a greater response in a bovine mammary cell proliferation.
Tou
J S
JS
Monsanto Company, St. Louis, Missouri 63198.
McGrath
M F
MF
Zupec
M E
ME
Byatt
J C
JC
Violand
B N
BN
Kaempfe
L A
LA
Vineyard
B D
BD
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D066246:ErbB Receptors / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000166:cytology; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D011487:Protein Conformation; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D015291:Transforming Growth Factors / Q000138:chemical synthesis* / Q000494:pharmacology
D007202:Indicators and Reagents; D015291:Transforming Growth Factors; D066246:ErbB Receptors