title: Transport of glutathione across the mitochondrial membranes.
abstract: Transport of glutathione (GSH) into mitochondria was observed when mitochondria in state 4 respiration were incubated with high concentrations of GSH. This transport was suppressed by antimycin A or dicyclohexyl-carbodiimide, or in state 3 respiration. Upon dissipation of the proton gradient by a proton ionophore, mitochondrial GSH was released into the medium. GSH moved freely across the proton-permeated mitochondrial membrane, its movement depending only on the GSH gradient across the inner membrane. These results indicate that there is a transport system for GSH in the mitochondrial membrane, and that a proton gradient is necessary to maintain GSH in the matrix, and to transport GSH into mitochondria.
authors.affiliation: Department of Physiological Chemistry, Osaka University Medical School, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kurosawa
authors.forename: N
authors.initials: N
authors.lastname: Hayashi
authors.forename: N
authors.initials: N
authors.lastname: Sato
authors.forename: T
authors.initials: T
authors.lastname: Kamada
authors.forename: K
authors.initials: K
authors.lastname: Tagawa
mesh_terms: D000818:Animals; D001692:Biological Transport; D005978:Glutathione / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism*; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism*; D010101:Oxygen Consumption; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D005978:Glutathione