A class I antigen, HLA-G, expressed in human trophoblasts.
The alpha chain of the human histocompatibility antigen HLA-G was identified as an array of five 37- to 39-kilodalton isoforms by the use of two-dimensional gel electrophoresis. Both cell-associated and secreted HLA-G antigens are prominent in first trimester villous cytotrophoblasts and are greatly reduced in third trimester cytotrophoblasts. Allelic variation was not detected, an indication that HLA-G is not obviously polymorphic in cytotrophoblasts. Among the following choriocarcinoma cell lines studied, HLA-G is expressed in JEG but not in Jar or BeWo. Expression of endogenous HLA-G genes has not been found in normal lymphoid cells. Thus, HLA-G is subject to both cell type-specific and developmental regulation and is expressed in early gestation human cytotrophoblasts.
Kovats
S
S
Department of Human Oncology, University of Wisconsin, Madison 53706.
Main
E K
EK
Librach
C
C
Stubblebine
M
M
Fisher
S J
SJ
DeMars
R
R
D000911:Antibodies, Monoclonal; D002460:Cell Line; D002822:Choriocarcinoma / Q000276:immunology; D005260:Female; D015870:Gene Expression; D005805:Genes, MHC Class I; D006680:HLA Antigens / Q000235:genetics*; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D046911:Macromolecular Substances; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D014327:Trophoblasts / Q000276:immunology*; D014407:Tumor Cells, Cultured / Q000276:immunology; D014594:Uterine Neoplasms / Q000276:immunology
D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; D046911:Macromolecular Substances