Skin grafting in gynogenetic common carp (Cyprinus carpio, L.). The development of histocompatible clones.
The fate of skin allografts exchanged among heterozygous and homozygous gynogenetic common carp siblings, and among newly developed inbred strains and F1 hybrids, is described. Heterozygous gynogenetic offspring were produced by fertilizing eggs with UV-irradiated sperm and by treating the resulting zygote with a cold shock (0 degree C, 45 min). The temperature shock causes retention of the second polar body, which allows the eggs to develop into normal diploid fry. Homozygous gynogenetic offspring were similarly produced by using a heat shock (40 degrees C, 2 min), which suppresses the first mitotic division. Skin allografts exchanged among heterozygous gynogenetic carp exhibited prolonged survival, with some allografts (21.8%) surviving for over 28 days. Furthermore, a strong histocompatibility locus was seen to segregate in this group. In contrast, skin allografts exchanged among homozygous gynogenetic siblings were all rejected within 14 days (MST 9.4 days). New homozygous inbred strains, designated JJ and MM, were produced by gynogenetic reproduction of homozygous female carps, while F1 hybrids were produced by crossing of these homozygous females with homozygous male siblings. All grafts exchanged among members of the same strain were permanently accepted. Likewise grafts from homozygous strain members were accepted by fish from the related F1-hybrids, while the reverse grafts were rejected. These results provide evidence for the idea that in the carp histocompatibility genes exist at least one major locus and multiple minor loci, which are codominantly expressed.
Komen
J
J
Department of Experimental Animal Morphology and Cell Biology, Agricultural University, Wageningen, The Netherlands.
Van den Dobbelsteen
P J
PJ
Slierendrecht
W J
WJ
Van Muiswinkel
W B
WB
D000818:Animals; D002347:Carps / Q000235:genetics / Q000276:immunology*; D003530:Cyprinidae / Q000276:immunology*; D005260:Female; D006084:Graft Rejection / Q000235:genetics; D007178:Inbreeding; D008285:Major Histocompatibility Complex / Q000235:genetics*; D008297:Male; D016038:Skin Transplantation / Q000276:immunology*