Assignment of two rat dihydrofolate (DHFR) genes to chromosomes 2 and 4.
A panel of rat x mouse cell hybrids was used in the chromosomal mapping of the rat dihydrofolate reductase (DHFR) gene. It was determined that the probe hybridized to gene sequences on two different chromosomes (Nos. 2 and 4), possibly representing the active gene and a pseudogene. Hybridization of the DHFR probe to DNA from a methotrexate resistant rat cell line revealed that the gene on chromosome 2 was amplified, but not the gene on chromosome 4. This result was taken to suggest that the active DHFR gene is located on rat chromosome 2 and that the sequence on chromosome 4 is a pseudogene.
Hanson
C
C
Department of Genetics, University of Gothenburg, Sweden.
Andersson
A
A
Islam
M Q
MQ
Szpirer
J
J
Szpirer
C
C
Levan
G
G
D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D006822:Hybrid Cells; D051379:Mice; D051381:Rats / Q000235:genetics*; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*
D013762:Tetrahydrofolate Dehydrogenase