title: Chromosome mapping using polymerase chain reaction on somatic cell hybrids.
abstract: DNA polymerase chain reaction (PCR) was used to detect specific human sequences in human-mouse somatic hybrids. As compared with classic Southern blotting, this technique appeared to be more sensitive and less time-consuming for gene mapping.
authors.affiliation: Center for Human Genetics, University of Leuven, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: K
authors.initials: K
authors.lastname: Devriendt
authors.forename: P
authors.initials: P
authors.lastname: Marynen
authors.forename: H
authors.initials: H
authors.lastname: Van den Berghe
authors.forename: J J
authors.initials: JJ
authors.lastname: Cassiman
mesh_terms: D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D002874:Chromosome Mapping; D005784:Gene Amplification; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011257:Pregnancy Proteins / Q000235:genetics
chemical_list: D011257:Pregnancy Proteins