Structural and regulatory analysis of the male-specific rat liver cytochrome P-450 g: repression by continuous growth hormone administration.
Complementary DNA clones encoding the male-specific rat liver cytochrome P-450 g have been isolated by cross-hybridization with sequences from the female-specific rat liver cytochrome P-450 15 beta. Tissue distribution analysis indicates the liver as the organ with major expression of this cytochrome P-450 gene. Minimal P-450 g expression was also detected in prostate, kidney, heart, and brain. A developmental analysis reveals liver expression in the 8-week-old male and to a lesser extent in the 4-week-old male, but no detectable expression is seen in females of these ages or in 1- and 2-week-old rats from both sexes. Hypophysectomy of female rats dramatically increases hepatic expression of P-450 g, whereas continuous GH administration represses hepatic expression in male or female hypophysectomized rats. In similarity to P-450 15 beta and P-450 16 alpha, therefore, the cytochrome P-450 g gene in liver is GH regulated.
Zaphiropoulos
P G
PG
Department of Medical Nutrition, Karolinska Institute, Huddinge University Hospital, Sweden.
Ström
A
A
Robertson
J A
JA
Gustafsson
J A
JA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000235:genetics*; D005260:Female; D006062:Gonadotropins / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D005810:Multigene Family; D009928:Organ Specificity; D011061:Poly A / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D012097:Repressor Proteins / Q000494:pharmacology*; D015183:Restriction Mapping; D014157:Transcription Factors / Q000494:pharmacology*
D006062:Gonadotropins; D012333:RNA, Messenger; D012097:Repressor Proteins; D014157:Transcription Factors; D011061:Poly A; D003577:Cytochrome P-450 Enzyme System