title: Detection of neuron specific enolase concentrations in cerebrospinal fluid from patients with neurological disorders by means of a sensitive enzyme immunoassay.
abstract: An enzyme linked immunosorbent assay (ELISA) for the detection of neuron specific enolase (NSE) in cerebrospinal fluid (CSF) was developed. The sensitivity of the ELISA was less than 1 microgram/ml. This sensitivity is comparable with radioimmunoassays which have the disadvantage that radiolabelled products are used. The developed assay was used to measure cerebrospinal fluid neuron specific enolase (CSF-NSE) levels in 1178 patients with neurological disorders to establish its potential usefulness and clinical application. CSF-NSE levels in this group of patients were independent of sex and no correlation with age was found. CSF-NSE was significantly increased in Creutzfeldt-Jacob disease, meningeal hemorrhage, thrombosis, Guillain-Barré syndrome and in schizophrenia.
authors.affiliation: Laboratory of Neurochemistry, Born-Bunge Foundation, U.I.A., Antwerp, Belgium.
authors.forename: K
authors.initials: K
authors.lastname: Vermuyten
authors.forename: A
authors.initials: A
authors.lastname: Lowenthal
authors.forename: D
authors.initials: D
authors.lastname: Karcher
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D008297:Male; D009422:Nervous System Diseases / Q000134:cerebrospinal fluid / Q000201:enzymology*; D010751:Phosphopyruvate Hydratase / Q000134:cerebrospinal fluid*; D011817:Rabbits; D011863:Radioimmunoassay; D012016:Reference Values; D012559:Schizophrenia / Q000134:cerebrospinal fluid / Q000201:enzymology; D012737:Sex Factors
chemical_list: D000911:Antibodies, Monoclonal; D010751:Phosphopyruvate Hydratase