Influence of aspirin and iron(III) tetrasulfonated phthalocyanine on bilirubin binding by human serum albumin.
The interaction of bilirubin with aspirin-modified human serum albumin (HSA) and the influence of iron tetrasulfonated phthalocyanine on bilirubin binding by the native protein has been studied by difference spectroscopy and circular dichroism measurements. Spectroscopic studies of the systems containing bilirubin and aspirin-modified HSA compared to the analogous systems with the native protein have shown that selective acetylation of albumin at lysine 199 inhibits bilirubin binding by this protein. In both cases, interaction between bilirubin and albumin leads to complex formation at a molar ratio of ligand to protein of 2:1. The studies of the reaction of bilirubin with fragments of albumin produced by reaction with CNBr have demonstrated that one of the strong bilirubin binding sites is located in the M fragment and is close to the high-affinity binding site of aspirin. The other one was found in fragment C. Acetylation of albumin brings about marked conformational change in the protein, which probably accounts for the decrease in its ability to react with anti-HSA antibody. Bilirubin does not change the secondary structure of albumin but, like aspirin, lowers its antigenicity. It has been suggested that the decrease in antigenic properties in this case results from cooperation of the closely neighboring antigenic and bilirubin-binding sites. The studies of the influence of iron(III) tetrasulfonated phthalocyanine on bilirubin binding by HSA suggest that there is no competition between strong sites for iron(III) tetrasulfonated phthalocyanine and bilirubin, but these compounds compete for some of the weaker sites.
Trynda
L
L
Institute of Chemistry, University of Wrocław, Poland.
Przywarska-Boniecka
H
H
Kościukiewicz
T
T
D000818:Animals; D000941:Antigens / Q000276:immunology; D001241:Aspirin; D001663:Bilirubin / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D002942:Circular Dichroism; D005290:Ferric Compounds; D006801:Humans; D007211:Indoles; D007700:Kinetics; D011487:Protein Conformation; D011817:Rabbits; D012709:Serum Albumin / Q000276:immunology*; D013053:Spectrophotometry
D000941:Antigens; D005290:Ferric Compounds; D007211:Indoles; D012709:Serum Albumin; C028418:tetrasulfophthalocyanine; D001241:Aspirin; D001663:Bilirubin