Molecular requirements for T cell activation by the staphylococcal toxic shock syndrome toxin-1.
The activation of Ag-specific, Ia molecule-restricted, TCR V beta 3+ T cell clones by staphylococcal toxic shock syndrome toxin-1 (TSST-1), was investigated. The results show that although Ag- and TSST-1-induced activation of T cell clones both require TCR expression and similar biologic activation signals, the Ia molecule requirement for TSST-1 recognition was much less stringent than that observed for antigenic peptide recognition. In addition, T cell clones recognized TSST-1 without processing by APC. These results suggest that the ability of TSST-1 to polyclonally activate T cells is dependent on TCR recognition of the intact toxin molecule bound to a nonpolymorphic region(s) of the Ia molecule resulting in the same activation events induced by Ag recognition.
Norton
S D
SD
Department of Microbiology, University of Minnesota Medical School, Minneapolis 55455.
Schlievert
P M
PM
Novick
R P
RP
Jenkins
M K
MK
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D001427:Bacterial Toxins; D002738:Chloroquine / Q000494:pharmacology; D002999:Clone Cells; D004768:Enterotoxins / Q000276:immunology* / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006825:Hybridomas; D066298:In Vitro Techniques; D007295:Inositol Phosphates / Q000378:metabolism; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D051379:Mice; D011948:Receptors, Antigen, T-Cell / Q000502:physiology; D013211:Staphylococcus aureus / Q000276:immunology; D018089:Superantigens; D013601:T-Lymphocytes / Q000276:immunology*
D001427:Bacterial Toxins; D004768:Enterotoxins; D000949:Histocompatibility Antigens Class II; D007295:Inositol Phosphates; D007376:Interleukin-2; D011948:Receptors, Antigen, T-Cell; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal; D002738:Chloroquine