Age-dependent changes in monkey lenticular gangliosides.
The content, composition, and distribution of gangliosides were examined in the lenses of normal rhesus monkeys aged 6-16 years. Gangliosides were isolated by organic solvent extraction. DEAE-Sephadex ion-exchange column chromatography, and thin-layer chromatography (TLC). Ganglioside contents determined by the thiobarbituric acid method increased in the lens with aging. TLC analysis of gangliosides showed a much more complex pattern with aging, and the predominant gangliosides were tentatively identified as GM3, GM1, and GD1a. Individual lenticular gangliosides were identified by TLC-immunostaining procedures using anti-GM1 and anti-asialoGM1 antisera.
Ogiso
M
M
Department of Physiology, Toho University School of Medicine, Tokyo, Japan.
Saito
N
N
Sudo
K
K
Hirano
S
S
Komoto
M
M
D000375:Aging / Q000378:metabolism*; D000818:Animals; D002855:Chromatography, Thin Layer; D005260:Female; D005732:Gangliosides / Q000032:analysis*; D007908:Lens, Crystalline / Q000032:analysis*; D008253:Macaca mulatta; D008297:Male
D005732:Gangliosides