title: Molecular cloning of the rinderpest virus matrix gene: comparative sequence analysis with other paramyxoviruses.
abstract: The nucleotide sequence of the gene encoding the matrix or membrane (M) protein of the virulent (Kabete-O) strain of rinderpest virus (RPV) has been determined. The M gene is 1457 nucleotides long with a single, large open reading frame. The derived polypeptide has 335 amino acids, corresponding to a calculated molecular weight of 38,289 and contains both small hydrophobic regions and many basic residues. The predicted amino acid sequence was compared to the M proteins of paramyxoviruses. Sequence comparison and hydropathy profiles among the morbilliviruses revealed that the M protein of RPV exhibits features similar to those of the M protein of MV and CDV. There is 78.2% homology at the amino acid level between the M protein of RPV and MV, and 77.6% between RPV and CDV. This indicates that a high degree of homology exists among the members of the genus Morbillivirus. In contrast, there is only 37.3 and 18% homology between RPV and bovine parainfluenza type 3 (BPV3), and RPV and Newcastle disease virus (NDV) M proteins, respectively. Thus the M proteins of the morbilliviruses are highly conserved whereas the M proteins of the genus Paramyxovirus show more divergence.
authors.affiliation: Department of Veterinary Microbiology and Immunology, University of California, Davis 95616.
authors.forename: M
authors.initials: M
authors.lastname: Limo
authors.forename: T
authors.initials: T
authors.lastname: Yilma
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D015723:Gene Library; D005814:Genes, Viral; D007668:Kidney / Q000382:microbiology; D008969:Molecular Sequence Data; D010252:Paramyxoviridae / Q000235:genetics*; D011487:Protein Conformation; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D012302:Rinderpest virus / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014763:Viral Matrix Proteins / Q000235:genetics*
chemical_list: D012367:RNA, Viral; D014763:Viral Matrix Proteins