Carbon catabolite regulation of phenylacetyl-CoA ligase from Pseudomonas putida.
Phenylacetyl-CoA ligase (PA-CoA ligase) from P. putida U is a newly described enzyme involved in the aerobic catabolism of phenylacetic acid. The enzyme was specifically induced when P. putida was grown in a chemically defined medium containing phenylacetic acid as the sole carbon source. The induction of PA-CoA ligase was delayed by adding easily metabolizable carbon sources to the medium; the effect was more drastic in the presence of glucose. Glucose did not cause catabolic inactivation but rather catabolic repression, this effect being reversed by cAMP.
Martinez-Blanco
H
H
Departamento Interfacultativo de Bioquimica y Biologia Molecular, Universidad de León, España.
Reglero
A
A
Luengo
J M
JM
D000332:Aerobiosis; D050260:Carbohydrate Metabolism; D002701:Chloramphenicol / Q000494:pharmacology; D003066:Coenzyme A Ligases / Q000096:biosynthesis*; D004790:Enzyme Induction; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D007700:Kinetics; D010648:Phenylacetates / Q000494:pharmacology*; D011549:Pseudomonas / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development
D010648:Phenylacetates; D002701:Chloramphenicol; D003066:Coenzyme A Ligases; C023914:phenylacetate - CoA ligase; C025136:phenylacetic acid; D005947:Glucose