Trophoblast glycoprotein recognised by monoclonal antibody 5T4 maps to human chromosome 6q14-q15.
Human X rodent hybrids were stained by indirect immunofluorescence with 5T4, a murine monoclonal antibody that recognises a 72 kdalton glycoprotein expressed by human trophoblasts and a very restricted range of adult tissues; they were analysed by flow cytometry. Concordance analysis supported by segregation data allowed assignment of the gene controlling glycoprotein expression (M6P1) to chromosome 6. Similar analysis with translocation hybrids gave a regional assignment to 6q14-q15. M6P1 is distinct from NT5, coding for 5' nucleotidase, which maps to the same region.
Boyle
J M
JM
Department of Biochemical Genetics, Paterson Institute of Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, UK.
Grzeschik
K H
KH
Heath
P R
PR
Morten
J E
JE
Stern
P L
PL
D000818:Animals; D000911:Antibodies, Monoclonal; D015415:Biomarkers / Q000032:analysis; D002871:Chromosome Banding; D002896:Chromosomes, Human, Pair 6; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000276:immunology; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000235:genetics* / Q000276:immunology; D014327:Trophoblasts / Q000276:immunology
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D005819:Genetic Markers; D008562:Membrane Glycoproteins; D011257:Pregnancy Proteins; C063709:trophoblastic glycoprotein 5T4, human