Recovery of infectious human immunodeficiency virus from cells treated with 1% paraformaldehyde.
Fixation and preservation of cells with 1% paraformaldehyde is a common practice in laboratories performing flow cytometric analysis of cells from persons infected with human immunodeficiency virus (HIV). Previous studies indicated that 1% paraformaldehyde effectively inactivates cell-free HIV. In this study, we tested the inactivation capacity of 1% paraformaldehyde on a cell line chronically infected with HIV and human peripheral blood lymphocytes acutely infected with the virus. We found a significant decrease in the infectious titer of the cells after treatment with 1% paraformaldehyde. However, we did not find total inactivation of the specimens treated for up to 18 h.
Aloisio
C H
CH
Centers for Disease Control, Department of Health and Human Services, Atlanta, GA 30333.
Nicholson
J K
JK
D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology; D002460:Cell Line; D002470:Cell Survival; D002478:Cells, Cultured; D005557:Formaldehyde / Q000494:pharmacology*; D006678:HIV / Q000187:drug effects* / Q000302:isolation & purification; D006801:Humans; D010835:Phytohemagglutinins / Q000494:pharmacology; D011108:Polymers / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000382:microbiology*; D013696:Temperature
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D010835:Phytohemagglutinins; D011108:Polymers; D005557:Formaldehyde; C003043:paraform