The spectrum of low molecular weight house dust mite (Dermatophagoides pteronyssinus) allergens with emphasis on Der p II.
Analysis by protein blotting of sera from 96 different house dust mite-allergic subjects revealed previously unrecognized complexity of low molecular weight (MW) (less than 20 kD) IgE-binding proteins in extracts of whole bodies of Dermatophagoides pteronyssinus. Of 11 different IgE-binding components of MW less than 20 kD identified, two (MW approximately 16 kD and approximately 15 kD), showed both a high frequency (88% and 49% respectively) and a high intensity of IgE-binding. The approximately 16 kD component, identified as allergen Der p II, showed the highest frequency of IgE antibody reactivity of any of the major D. pteronyssinus allergens including Der p I and Der p III.
Ford
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SA
Kolling Institute of Medical Research, Royal North Shore Hospital, St Leonards, Australia.
Tovey
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ER
Baldo
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BA
D000485:Allergens / Q000032:analysis* / Q000276:immunology; D000818:Animals; D039741:Antigens, Dermatophagoides; D004391:Dust; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D008222:Lymphokines / Q000032:analysis; D008970:Molecular Weight; D029584:Prostatic Secretory Proteins
D000485:Allergens; D039741:Antigens, Dermatophagoides; D004391:Dust; D008222:Lymphokines; D029584:Prostatic Secretory Proteins; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors