Glycoconjugate pattern of membranes in the acinar cell of the rat pancreas.
Lectin-binding studies were performed at the ultrastructural level to characterize glycoconjugate patterns on membrane systems in pancreatic acinar cells of the rat. Five lectins reacting with different sugar moieties were applied to ultrathin frozen sections: concanavalin A (ConA): glucose, mannose; wheat-germ agglutinin (WGA): N-acetylglucosamine, sialic acid; Ricinus communis agglutinin I (RCA I): galactose; Ulex europaeus agglutinin I (UEA I): L-fucose; soybean agglutinin (SBA): N-acetylgalactosamine). Binding sites of lectins were visualized either by direct conjugation to colloidal gold or by the use of a three-step procedure involving additional immune reactions. The rough endoplasmic reticulum and the nuclear envelope of acinar cells was selectively labelled for ConA. The membranes of the Golgi apparatus bound all lectins applied with an increasing intensity proceeding from the cis- to the trans-Golgi area for SBA, UEA I and WGA. In contrast RCA I selectively labelled the trans-Golgi cisternae. The membranes of condensing vacuoles and zymogen granules were labelled for all lectins used although the density of the label differed between the lectins. In contrast the content of zymogen granules failed to bind SBA and WGA. Lysosomal bodies (membranes and content) revealed binding sites for all lectins used. The plasma membranes were heavily labelled by all lectins except for SBA which showed only a weak binding to the lateral and the apical plasma membrane. These results are in accordance to current biochemical knowledge of the successive steps in the glycosylation of membrane proteins. It could be demonstrated, that the cryo-section technique is suitable for the fine structural localisation of surface glycoconjugates of plasma membranes and internal membranes in pancreatic acinar cells using plant lectins.
Willemer
S
S
Department of Internal Medicine, Philipps-University, Marburg, Federal Republic of Germany.
Köhler
H
H
Naumann
R
R
Kern
H F
HF
Adler
G
G
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D006001:Glycoconjugates / Q000032:analysis*; D006651:Histocytochemistry; D007425:Intracellular Membranes / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism*; D008297:Male; D010179:Pancreas / Q000648:ultrastructure*; D051381:Rats; D011919:Rats, Inbred Strains
D006001:Glycoconjugates; D037102:Lectins