Parameters involved in the in vitro immunization of tonsilar lymphocytes: effects of rIL-2 and muramyl dipeptide.
A method for the in vitro immunization of human tonsilar lymphocytes is described. This method allows for the sensitization of B-cells against various antigens under serum-free medium conditions. The medium is supplemented with defined concentrations of antigen, rIL-2 and muramyl dipeptide to boost the immune responses. This method may prove useful for the generation of antigen reactive B-cells for use in human monoclonal antibody production and the study of the effects of other lymphokines on the in vitro immune response.
Carroll
K
K
Bio Research Ireland, Dublin City University.
Prosser
E
E
O'Kennedy
R
R
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000494:pharmacology*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000941:Antigens / Q000008:administration & dosage; D003470:Culture Media; D006801:Humans; D007114:Immunization; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000494:pharmacology*; D008214:Lymphocytes / Q000276:immunology*; D014066:Palatine Tonsil / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors
D000911:Antibodies, Monoclonal; D000941:Antigens; D003470:Culture Media; D007376:Interleukin-2; D000119:Acetylmuramyl-Alanyl-Isoglutamine