The gene for the alpha 2 chain of the human fibrillar collagen type XI (COL11A2) assigned to the short arm of chromosome 6.
A cosmid clone (CosHcol.11) containing the alpha 2(XI) collagen gene (COL11A2) has been isolated. The gene contains conserved DNA and amino-acid sequences characteristic of fibril forming collagen, which is in accordance with the classification of type XI collagen as a fibrillar collagen. The genomic clone containing the alpha 2(XI) gene has been used as probe in the Southern blot analysis of DNA from a panel of human/hamster somatic cell hybrids containing different numbers and combinations of human chromosomes. Synteny analysis revealed that only chromosome 6 showed complete concordant segregation with COL11A2. Furthermore, the gene was regionally mapped to the short arm of chromosome 6 by using a hybrid which contained only the long arm of the chromosome.
Law
M L
ML
Eleanor Roosevelt Institute for Cancer Research, University of Colorado, Health Sciences Center, Denver 80206.
Chan
S D
SD
Berger
R
R
Jones
C
C
Kao
F T
FT
Solomon
E
E
Cheah
K S
KS
D015139:Blotting, Southern; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6; D003094:Collagen / Q000235:genetics*; D004247:DNA; D005109:Extracellular Matrix; D006801:Humans; D009693:Nucleic Acid Hybridization
D003094:Collagen; D004247:DNA