Evidence that the level of the p55 component of the interleukin (IL) 2 receptor can control IL 2 responsiveness in a murine IL 3-dependent cell.
Although the role of interleukin 2 (IL 2) in mature T lymphocyte function is well documented, its effect on hematopoietic progenitor cells is less well characterized. Here we have used recombinant retroviruses to transduce and express a cDNA clone encoding the p55 component of the human IL 2 receptor (h-p55), in a murine IL 3-dependent cell line, BAF3. While the parental cells do not respond to IL 2, the h-p55-expressing cells proliferate upon treatment with recombinant IL 2 after an initial lag period. The responsiveness of individual cell clones is correlated with their level of h-p55 expression, and can be inhibited by Tac monoclonal antibody. Furthermore, growth at limiting IL 2 concentrations selects a subset of cells expressing higher h-p55 levels from a bulk population. Detailed 125I-labeled IL 2 binding analysis on the highest h-p55-expressing clone detects the presence of 200 high-affinity (KD = 25 pM) IL 2 receptors. We therefore propose that the level of h-p55 expression governs the formation of high-affinity receptors, and hence IL 2 responsiveness in BAF3 cells.
Collins
M K
MK
Whitehead Institute, Cambridge, London, GB.
Malde
P
P
Miyajima
A
A
Arai
K
K
Smith
K A
KA
Mulligan
R C
RC
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000502:physiology*; D007377:Interleukin-3 / Q000502:physiology; D008214:Lymphocytes / Q000502:physiology*; D046911:Macromolecular Substances; D051379:Mice; D008970:Molecular Weight; D015375:Receptors, Interleukin-2 / Q000502:physiology*; D011994:Recombinant Proteins; D014162:Transfection
D007376:Interleukin-2; D007377:Interleukin-3; D046911:Macromolecular Substances; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins