Cloning and characterization of a gene encoding a new thermostable hemolysin from Vibrio parahaemolyticus.
A new thermostable hemolysin (delta-VPH) gene was cloned from a Kanagawa-negative Vibrio parahaemolyticus strain into vector pBR322 in Escherichia coli K12. The nucleotide and amino acid sequences had no homology with those of the thermostable direct hemolysin (TDH) which causes the Kanagawa phenomenon, and of the thermolabile hemolysin (TLH) of V. parahaemolyticus. The gene was present in all V. parahaemolyticus strains tested and also in one strain of V. damsela.
Taniguchi
H
H
Department of Microbiology, School of Medicine, University of Environmental and Occupational Health, Fukuoka, Japan.
Kubomura
S
S
Hirano
H
H
Mizue
K
K
Ogawa
M
M
Mizuguchi
Y
Y
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D013696:Temperature; D014736:Vibrio parahaemolyticus / Q000235:genetics*
D004269:DNA, Bacterial; D006460:Hemolysin Proteins