title: A novel anthelmintic model utilizing jirds, Meriones unguiculatus, infected with Haemonchus contortus.
abstract: Currently, no in vivo laboratory model is available for evaluating anthelmintics against the important ruminant helminth Haemonchus contortus. This report outlines a novel anthelmintic assay utilizing immunosuppressed (0.02% hydrocortisone in feed) jirds, Meriones unguiculatus, infected with H. contortus. Immunosuppressed jirds were inoculated with approximately 1,000 exsheathed infective larvae of H. contortus, treated per os on day 10 postinoculation (PI), and necropsied on day 13 PI. Each stomach was removed, opened longitudinally, incubated in distilled water at 37 C for 5 hr, fixed in formaldehyde solution, and stored for subsequent examination. Stomach contents were examined using a stereomicroscope (15-45x). A variety of standard anthelmintics has been evaluated in the model; modern broad-spectrum ruminant anthelmintics (benzimidazoles, febantel, ivermectin, levamisole hydrochloride, and milbemycin D) are active uniformly and in most cases at doses (mg/kg) comparable to those required for efficacy against H. contortus in ruminants. This model provides an important new tool to assess preliminarily the activity of experimental drugs against H. contortus in vivo prior to studies in ruminants and also may provide a useful tool for studying host-parasite interactions for H. contortus.
authors.affiliation: Parasitology Research, Upjohn Company, Kalamazoo, Michigan 49001.
authors.forename: G A
authors.initials: GA
authors.lastname: Conder
authors.forename: L W
authors.initials: LW
authors.lastname: Jen
authors.forename: K S
authors.initials: KS
authors.lastname: Marbury
authors.forename: S S
authors.initials: SS
authors.lastname: Johnson
authors.forename: P M
authors.initials: PM
authors.lastname: Guimond
authors.forename: E M
authors.initials: EM
authors.lastname: Thomas
authors.forename: B L
authors.initials: BL
authors.lastname: Lee
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005260:Female; D005849:Gerbillinae; D006188:Haemonchiasis / Q000188:drug therapy* / Q000469:parasitology; D006790:Host-Parasite Interactions; D014252:Trichostrongyloidiasis / Q000188:drug therapy*
chemical_list: D000871:Anthelmintics