A novel chromatographic resolution of 21-hydroxysteroid metabolites of progesterone.
21-Hydroxysteroid metabolites of both progesterone and deoxycorticosterone are excreted in rabbit urine and are eluted from an alumina adsorption column after 21-deoxysteroids. The separation is independent of polarity and dependent on the interaction of the 21-hydroxyl function with the adsorbent. The group separation of these steroids allowed further analysis by high performance liquid chromatography and revealed different proportions of metabolites. This is the first report of the excretion of 21-hydroxysteroid metabolites of progesterone in rabbit urine.
Senciall
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Faculty of Medicine, Memorial University of Newfoundland, St. John's, Canada.
Roberts
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Rahal
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D000537:Aluminum Oxide; D000818:Animals; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D003900:Desoxycorticosterone / Q000652:urine; D005965:Glucuronates / Q000652:urine; D006914:Hydroxysteroids / Q000652:urine*; D011374:Progesterone / Q000652:urine*; D011817:Rabbits
D005965:Glucuronates; D006914:Hydroxysteroids; D003900:Desoxycorticosterone; D011374:Progesterone; D000537:Aluminum Oxide