Recombinant human bone morphogenetic protein induces bone formation.
We have purified and characterized active recombinant human bone morphogenetic protein (BMP) 2A. Implantation of the recombinant protein in rats showed that a single BMP can induce bone formation in vivo. A dose-response and time-course study using the rat ectopic bone formation assay revealed that implantation of 0.5-115 micrograms of partially purified recombinant human BMP-2A resulted in cartilage by day 7 and bone formation by day 14. The time at which bone formation occurred was dependent on the amount of BMP-2A implanted; at high doses bone formation could be observed at 5 days. The cartilage- and bone-inductive activity of the recombinant BMP-2A is histologically indistinguishable from that of bone extracts. Thus, recombinant BMP-2A has therapeutic potential to promote de novo bone formation in humans.
Wang
E A
EA
Genetics Institute, Cambridge, MA 02140.
Rosen
V
V
D'Alessandro
J S
JS
Bauduy
M
M
Cordes
P
P
Harada
T
T
Israel
D I
DI
Hewick
R M
RM
Kerns
K M
KM
LaPan
P
P
D000818:Animals; D001846:Bone Development / Q000187:drug effects*; D019485:Bone Morphogenetic Proteins; D001842:Bone and Bones / Q000166:cytology; D002356:Cartilage / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*; D002460:Cell Line; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D008970:Molecular Weight; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000494:pharmacology
D019485:Bone Morphogenetic Proteins; D006133:Growth Substances; D011506:Proteins; D011994:Recombinant Proteins