title: Rearrangement of VHa1-encoding Ig gene segment to the a2 chromosome in an a1/a2 heterozygous rabbit. Evidence for trans recombination.
abstract: VDJ genes were cloned from leukemic B cells of an a1/a2 heterozygous Emu-cmyc transgenic rabbit. Restriction mapping and nucleotide sequence analysis indicated that one clone, 5C3, had a VHa1-encoding gene segment functionally rearranged to a JH gene segment from the a2 chromosome. This VDJ gene may be the result of a trans recombination between a VH gene on the a1 chromosome and a JH gene segment on the a2 chromosome or, it may be the result of a cis recombination if the a2 chromosome contains VHa1-encoding gene segments.
authors.affiliation: Department of Microbiology, University of Illinois, Chicago 60680.
authors.forename: M
authors.initials: M
authors.lastname: Suter
authors.forename: R S
authors.initials: RS
authors.lastname: Becker
authors.forename: K L
authors.initials: KL
authors.lastname: Knight
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D005803:Genes, Immunoglobulin; D007126:Immunoglobulin Allotypes / Q000235:genetics*; D008969:Molecular Sequence Data; D011817:Rabbits / Q000235:genetics* / Q000276:immunology; D011995:Recombination, Genetic; D015183:Restriction Mapping
chemical_list: D007126:Immunoglobulin Allotypes