A comparison of different cloned genome segments of epizootic haemorrhagic disease virus as serogroup-specific probes.
The eight largest double-stranded (ds) RNA genome segments of epizootic haemorrhagic disease virus (EHDV) serotype 2 (Alberta strain) have been cloned. Of these, segments 4, 5, 6, 7, and 8 are represented by clones that correspond in size to those predicted for full-length clones. The different clones were used as nucleic acid probes to study the nucleic acid homology of cognate genes of four different EHDV serotypes. The results indicated that the 4 isolates may be subdivided in two geographic groups which include Ibaraki virus as the sole member of one group and EHDV1 (New Jersey), EHDV2 (Alberta) and EHDV6 (XBM 67--isolated in South Africa) as a second. Genome segments 1, 3, 4, 6, and 8 were found to be highly conserved with more than 90% homology amongst cognate genes of three of the members of the EHDV serogroup. Of these, segments 1 and 3 showed the largest degree of homology with cognate genes of members of the BTV serogroup. Segment 6 and 8 probes of EHDV2 (Alberta) on the other hand did not hybridize with BTV dsRNA under conditions of moderate to low stringency and are recommended for use as EHDV group-specific probes.
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Department of Microbiology, University of Pretoria, Republic of South Africa.
Huismans
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D001345:Autoradiography; D003001:Cloning, Molecular / Q000379:methods; D005814:Genes, Viral; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D012330:RNA, Double-Stranded / Q000235:genetics / Q000302:isolation & purification; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D012087:Reoviridae / Q000145:classification / Q000235:genetics*; D012703:Serotyping
D015347:RNA Probes; D012330:RNA, Double-Stranded; D012367:RNA, Viral