Evaluation of horseradish peroxidase as an intracellular stain in electrophysiological recording.
Iontophoresis of horseradish peroxidase into cells during intracellular electrophysiological recording to visualize the neurons recorded from is a powerful technique, but its success depends on a number of critical factors. The described agar injection technique is a simple method for the evaluation of several parameters at once, using procedures which closely resemble recording from, fixing, and processing to visualize the HRP in experimental tissue.
Ball
A
A
Division of Anatomy and Experimental Morphology, McMaster University, Hamilton, Ont., Canada.
Ammermüller
J
J
Weiler
R
R
D004594:Electrophysiology / Q000379:methods*; D006651:Histocytochemistry / Q000379:methods*; D006735:Horseradish Peroxidase; D010544:Peroxidases
D006735:Horseradish Peroxidase; D010544:Peroxidases