title: Characteristic immunophenotyping artefact seen in patients with anti-mouse immunoglobulin antibodies.
abstract: Patients treated with murine monoclonal antibodies develop anti-mouse immunoglobulin antibodies which can interfere with flow cytometry of lymphocytes, giving rise to high levels of nonspecific staining. This artefact can be avoided by using separated, washed lymphocytes for immunophenotyping.
authors.affiliation: Retroviral Research Group, Clinical Research Centre, Harrow.
authors.forename: D
authors.initials: D
authors.lastname: Wilks
authors.forename: N
authors.initials: N
authors.lastname: Byrom
authors.forename: L
authors.initials: L
authors.lastname: Walker
authors.forename: J
authors.initials: J
authors.lastname: Habeshaw
authors.forename: A
authors.initials: A
authors.lastname: Dalgleish
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000627:therapeutic use; D015704:CD4 Antigens / Q000276:immunology*; D005434:Flow Cytometry; D015658:HIV Infections / Q000276:immunology / Q000628:therapy*; D006801:Humans; D007167:Immunotherapy; D051379:Mice; D010641:Phenotype
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D015704:CD4 Antigens