title: Separation of oligosaccharides by capillary supercritical fluid chromatography and analysis by direct coupling to high-resolution mass spectrometer: application to analysis of oligomannosidic N-glycans.
abstract: Supercritical fluid chromatography separations and supercritical fluid chromatography chemical ionization mass spectrometry analysis of permethylated and pertrimethylsilylated oligosaccharides are reported. Supercritical fluid chromatography was carried out using a DB-5 coated capillary column with carbon dioxide as a mobile phase. Peralkylated oligosaccharides were detected by flame ionization and by chemical ionization mass spectrometry using the GC interface. Analysis of permethylated malto-oligosaccharides, as well as oligomannosides from mannosidosis, was achieved by chemical ionization mass spectrometry with ammonia and provided the pseudo-molecular ions (M+H)+ and (M+NH4)+, in addition to some other fragments which allow interpretations of the structure of different oligosaccharides. The good resolution and sensitivity obtained emphasize the potential of supercritical fluid chromatography mass spectrometry for rapid separations and analysis of complex glycan mixtures.
authors.affiliation: Laboratoire de Chimie Biologique de l'Université des Sciences et Techniques de Lille Flandres-Artois, Villeneuve d'Ascq, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Leroy
authors.forename: J
authors.initials: J
authors.lastname: Lemoine
authors.forename: G
authors.initials: G
authors.lastname: Ricart
authors.forename: J C
authors.initials: JC
authors.lastname: Michalski
authors.forename: J
authors.initials: J
authors.lastname: Montreuil
authors.forename: B
authors.initials: B
authors.lastname: Fournet
mesh_terms: D002845:Chromatography; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation; D009844:Oligosaccharides / Q000302:isolation & purification*; D011134:Polysaccharides / Q000302:isolation & purification
chemical_list: D009844:Oligosaccharides; D011134:Polysaccharides; C028569:oligomannoside