DEL cell line: a "malignant histiocytosis" CD30+ t(5;6)(q35;p21) cell line.
A new cell line DEL, established in vitro, was isolated from a pleural effusion of a boy who died of malignant histiocytosis. Its principal characteristics are: strong positivity with monoclonal antibodies (MAbs) to CD25, CD30, CD45R, KiM7, EMA, HLA Cl I and II; constant presence of acid phosphatase, ANAE, alpha-anti-trypsin, alpha-anti-chymotrypsin and NBT reductase activity; rearrangement of the immunoglobulin heavy-chain gene (JH) and a germ-line configuration of the T-chain gene; and finally a translocation between chromosomes 5-6 with a breakpoint in 5q35. The DEL cell line is appropriate for studying the role of the 5q localized c-fms oncogene and of the genes of the mononuclear phagocyte growth factor (CSFI) and of their receptors in the dynamics and etiology of malignant hemopathies associated with a 5q35 breakpoint.
Barbey
S
S
Groupe de Pathologie Pédiatrique, Hôpital Necker Enfants Malades, Paris, France.
Gogusev
J
J
Mouly
H
H
Le Pelletier
O
O
Smith
W
W
Richard
S
S
Soulie
J
J
Nezelof
C
C
D000951:Antigens, Neoplasm / Q000032:analysis; D002648:Child; D054747:Histiocytic Sarcoma / Q000276:immunology / Q000473:pathology*; D006801:Humans; D007621:Karyotyping; D008297:Male; D008854:Microscopy, Electron; D014407:Tumor Cells, Cultured / Q000276:immunology / Q000473:pathology
D000951:Antigens, Neoplasm