Pharmaco-toxicological effects of acetaminophen in rodents. Battery of tests to screen potential analgesic acetaminophen derivatives.
The pharmacological effects of acute oral administration of acetaminophen have been extensively evaluated in rodents. Using this drug as reference compound we have also standardized several pharmaco-toxociological tests in order to select new analgesic-antipyretic acetaminophen derivatives. According to the results obtained in this work, the following battery of assays is proposed to screen these compounds: a) pharmacological: antipyretic activity against Brewer's yeast-induced pyresis in rats; analgesic activity against chemical-induced writhings in mice and paw pressure test in rats; b) toxicological: oral acute toxicity in mice with and without phenobarbital pretreatment; determination of SGOT and SGPT activities as well as total bilirubin in mice. The usefulness of this procedure in the evaluation of drug effects is also considered.
Guasch
J
J
Research Center, Department of Pharmacology, Sant Feliu de Llobregat, Barcelona, Spain.
Grau
M
M
Montero
J L
JL
Felipe
A
A
D000082:Acetaminophen / Q000031:analogs & derivatives* / Q000494:pharmacology* / Q000633:toxicity; D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal; D001219:Aspartate Aminotransferases / Q000097:blood; D016227:Benzoquinones; D056486:Chemical and Drug Induced Liver Injury / Q000503:physiopathology; D004558:Electric Stimulation; D005260:Female; D008297:Male; D051379:Mice; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy; D011809:Quinones; D051381:Rats; D011919:Rats, Inbred Strains; D012835:Silver Nitrate
D000894:Anti-Inflammatory Agents, Non-Steroidal; D016227:Benzoquinones; D011809:Quinones; D000082:Acetaminophen; C029322:phenylbenzoquinone; D012835:Silver Nitrate; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase