Effect of tulipin on cell cycle progression analyzed by BrdUrd incorporation.
The effect of tulipin, a protein from plant origin recently purified, on cell cycle progression has been analyzed in the sensitive EUE cells by BrdUrd incorporation. The cytofluorimetric results demonstrate that tulipin specifically interacts with the S phase, with a dose-dependent decrease of the total S phase cells and an increase of the G1/G2 cells after 4 h of treatment in the synchronized EUE cells, whereas in the asynchronous population it mainly causes a dose-dependent decrease in the incorporation of BrdUrd per cell.
Vitale
M
M
Istituto di Anatomia Umana Normale, Università di Bologna, Italia.
Papa
S
S
Roncuzzi
L
L
Gasperi-Campani
A
A
D001973:Bromodeoxyuridine / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006023:Glycoproteins; D006801:Humans; D010940:Plant Proteins / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000166:cytology
D006023:Glycoproteins; D010940:Plant Proteins; C052558:tulipin protein, Tulipa; D001973:Bromodeoxyuridine