Protein content and enzyme activities in methanol- and acetate-grown Methanosarcina thermophila.
The cell extract protein content of acetate- and methanol-grown Methanosarcina thermophila TM-1 was examined by two-dimensional polyacrylamide gel electrophoresis. More than 100 mutually exclusive spots were present in acetate- and methanol-grown cells. Spots corresponding to acetate kinase, phosphotransacetylase, and the five subunits of the carbon monoxide dehydrogenase complex were identified in acetate-grown cells. Activities of formylmethanofuran dehydrogenase, formylmethanofuran:tetrahydromethanopterin formyltransferase, 5,10-methenyltetrahydromethanopterin cyclohydrolase, methylene tetrahydromethanopterin:coenzyme F420 oxidoreductase, formate dehydrogenase, and carbonic anhydrase were examined in acetate- and methanol-grown Methanosarcina thermophila. Levels of formyltransferase in either acetate- or methanol-grown Methanosarcina thermophila were approximately half the levels detected in H2-CO2-grown Methanobacterium thermoautotrophicum. All other enzyme activities were significantly lower in acetate- and methanol-grown Methanosarcina thermophila.
Jablonski
P E
PE
Department of Anaerobic Microbiology, Virginia Polytechnic Institute and State University, Blacksburg 24061-0305.
DiMarco
A A
AA
Bobik
T A
TA
Cabell
M C
MC
Ferry
J G
JG
D000085:Acetates / Q000378:metabolism*; D001426:Bacterial Proteins / Q000302:isolation & purification*; D015180:Electrophoresis, Gel, Two-Dimensional; D019605:Euryarchaeota / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D000432:Methanol / Q000378:metabolism*; D013045:Species Specificity
D000085:Acetates; D001426:Bacterial Proteins; D000432:Methanol