title: Force measurements from voltage-clamped guinea pig ventricular myocytes.
abstract: We describe the first observations of isolated mammalian guinea pig ventricular myocytes that combine measurements of contractile force with the voltage-clamp method. The myocytes were attached by poly-L-lysine to the beveled ends of a pair of thin glass rods having a compliance of 0.76 m/N. The contractile force of a cell caused a 1- to 3-microm displacement of the rods; the motion of which was converted to an output voltage by phototransistors. By the use of the whole cell patch-clamp technique, the cells were depolarized at 1 Hz with 200-ms-long clamp pulses from -45 to +5 mV (35 degrees C, 3.6 mM CaCl2). Isometric force began after a latency of 7 +/- 2 ms, peaked at 93 +/- 21 ms, and relaxed (90%) at 235 +/- 63 ms. The time course of force was always faster than that of isotonic shortening (time to peak 154 +/- 18 ms). With 400-ms-long depolarizations, a tonic component was recorded as either sustained force or sustained shortening that decayed on repolarization. Substitution of Ca by Sr in the bath increased the inward current through Ca channels but slowed down the time course of force development. The results are consistent with the hypothesis that activator calcium derives mainly from internal stores and that Ca release needs Ca entry through channels.
authors.affiliation: Department of Physiology, University of Cologne, Federal Republic of Germany.
authors.forename: N
authors.initials: N
authors.lastname: Shepherd
authors.forename: M
authors.initials: M
authors.lastname: Vornanen
authors.forename: G
authors.initials: G
authors.lastname: Isenberg
mesh_terms: D000287:Administration, Topical; D000818:Animals; D004594:Electrophysiology; D006168:Guinea Pigs; D006321:Heart / Q000502:physiology*; D006352:Heart Ventricles; D009200:Myocardial Contraction; D009206:Myocardium / Q000166:cytology; D011107:Polylysine / Q000494:pharmacology; D013997:Time Factors
chemical_list: D011107:Polylysine