DNA cytometric and histologic findings in mouse tumors (BP and S 180) with different response to treatment with tumor necrosis factor.
DNA cytometric and histopathologic investigations were performed in two tumor models (BP and S 180) which differed in their sensitivity to tumor necrosis factor (TNF). TNF induced strong necrosis in both tumors, but only the sarcoma 180 showed total regression. After TNF administration DNA cytometry revealed in the BP tumor an increase of cells in the S-phase, and in the S 180 tumor a loss of aneuploid cell populations. Histologic examination revealed a more obvious effect of TNF on tumor blood vessels in BP tumor, whereas infiltration of inflammatory cells was observed only in the S 180 tumor. We concluded that cell infiltration may be of importance for tumor regression and that aneuploid cell populations are more sensitive to TNF treatment than eudiploid cells.
Rychly
J
J
Department of Internal Medicine, Wilhelm-Pieck-University, Rostock, East Germany.
Knippel
E
E
Krygier-Stojalowska
A
A
Nizze
H
H
Kuchnio
M
M
Kraeft
S K
SK
D000818:Animals; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D051379:Mice; D008808:Mice, Inbred CBA; D008813:Mice, Inbred ICR; D009336:Necrosis; D011003:Ploidies; D012510:Sarcoma 180 / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D012513:Sarcoma, Experimental / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D014409:Tumor Necrosis Factor-alpha / Q000627:therapeutic use*
D004273:DNA, Neoplasm; D014409:Tumor Necrosis Factor-alpha