title: Species-specific oligodeoxynucleotide probes for the identification of periodontal bacteria.
abstract: Oligodeoxynucleotide probes were developed for identification of the periodontal bacteria Actinobacillus actinomycetemcomitans, Bacteroides gingivalis, B. intermedius types I and II, B. forsythus, Eikenella corrodens, Fusobacterium nucleatum, Haemophilus aphrophilus, Streptococcus intermedius, and Wolinella recta. Probes were designed by sequencing the 16S rRNA for each bacterium, identifying hypervariable regions, and chemically synthesizing species-specific probes. These probes were specific when tested against a panel of nucleic acids from closely related bacteria.
authors.affiliation: Molecular Biology Division, MicroProbe Corporation, Bothell, Washington 98021.
authors.forename: K
authors.initials: K
authors.lastname: Dix
authors.forename: S M
authors.initials: SM
authors.lastname: Watanabe
authors.forename: S
authors.initials: S
authors.lastname: McArdle
authors.forename: D I
authors.initials: DI
authors.lastname: Lee
authors.forename: C
authors.initials: C
authors.lastname: Randolph
authors.forename: B
authors.initials: B
authors.lastname: Moncla
authors.forename: D E
authors.initials: DE
authors.lastname: Schwartz
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D015342:DNA Probes; D003773:Dental Plaque / Q000382:microbiology; D006801:Humans; D008969:Molecular Sequence Data; D009055:Mouth / Q000382:microbiology; D010510:Periodontal Diseases / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity
chemical_list: D015342:DNA Probes; D012336:RNA, Ribosomal, 16S