Inhibition of Candida adhesion to buccal epithelial cells by an aqueous extract of Allium sativum (garlic).
The effect of pre-incubation of either Candida or buccal epithelial cells (BEC) with different concentrations of aqueous garlic extract (AGE) was investigated, as well as the effect of mouth rinse with AGE on the adhesion of yeast to BEC. Adhesion of Candida spp. to BEC was significantly reduced after both short and long time exposure of yeast to AGE. A similar inhibition of adherence was observed upon preincubation of BEC with AGE. The adherence-inhibition activity of AGE treatment was antagonized by thiols such as L-cysteine, glutathione and 2-mercaptoethanol. In addition, germ-tube formation was suppressed when C. albicans cells were pretreated with AGE. There was a significant reduction in the adherence of yeasts to BEC collected immediately or 15 min after an oral rinse with AGE. No statistical significance in the adhesion of BEC collected 30 min after oral rinse with AGE and control BEC was observed. The diminished adherence of C. albicans to BEC after exposure to various concentrations of garlic may have clinical relevance.
Ghannoum
M A
MA
Department of Botany and Microbiology, Faculty of Science, Kuwait University, Safat.
D001422:Bacterial Adhesion / Q000187:drug effects*; D002175:Candida / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005737:Garlic; D006801:Humans; D008297:Male; D009061:Mouth Mucosa / Q000166:cytology / Q000382:microbiology*; D009067:Mouthwashes / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal
D009067:Mouthwashes; D010936:Plant Extracts