CD4-like molecules in human sperm.
The expression of a molecule recognized by CD4 monoclonal antibodies was investigated on human sperm using immunolabelling, biochemical and immunochemical methods. Flow cytometry detected a significant fluorescence signal. SDS-PAGE analysis and Western blotting identified a molecule of 60 kDa, consistent with a CD4-like structure as confirmed after selective immunoseparation. Additional bands reacting with anti-CD4 were found in sperm extracts (73 kDa) and seminal fluid (90 kDa). These data indicate that sperm express a molecule similar to the receptor for HIV described on mononuclear cells.
Gobert
B
B
Laboratoire d'Immunologie, Faculté de Médecine, Université de Nancy, Vandoeuvre-les Nancy, France.
Amiel
C
C
Tang
J Q
JQ
Barbarino
P
P
Béné
M C
MC
Faure
G
G
D015703:Antigens, CD / Q000032:analysis*; D015153:Blotting, Western; D015704:CD4 Antigens / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D006801:Humans; D007526:Isoelectric Point; D008297:Male; D008970:Molecular Weight; D013094:Spermatozoa / Q000276:immunology*
D015703:Antigens, CD; D015704:CD4 Antigens