The distribution of [14C]acrylamide in rainbow trout studied by whole-body autoradiography.
The distribution of [2,3-14C]acrylamide was studied in fingerling rainbow trout by whole-body autoradiography. Fish weighing approximately 7 g were injected ip with 3.2 mg/kg [14C]acrylamide (0.1 microCi/g). One group of fish was kept in a fresh flowing water tank and frozen in dry ice/hexane 22 hr after injection; another group was placed in a separate tank of fresh flowing water and frozen 120 hr after treatment. A third group of fish served as nontreated controls. The autoradiographs of the fish at 22 hr show the highest concentration of radioactivity in the kidney, urinary bladder, blood, gallbladder, intestinal contents, and lens of eye. Lesser amounts of radioactivity are seen in the CNS, liver, and gills. Very low concentrations are seen in muscle. By 120 hr the only high concentrations are seen in gallbladder and lens of the eye. Lesser amounts are seen in the sclera, vertebrae, CNS, kidney, wall of intestine, and discrete spots in subcutaneous tissue presumed to be chromatophores. Low amounts are seen in muscle, the tissue usually consumed by man.
Waddell
W J
WJ
Department of Pharmacology and Toxicology, University of Louisville, Kentucky 40292.
Lech
J J
JJ
Marlowe
C
C
Kleinow
K M
KM
Friedman
M A
MA
D020106:Acrylamide; D000178:Acrylamides / Q000493:pharmacokinetics*; D000818:Animals; D001345:Autoradiography; D002250:Carbon Radioisotopes; D012487:Salmonidae / Q000378:metabolism*; D014018:Tissue Distribution; D014337:Trout / Q000378:metabolism*
D000178:Acrylamides; D002250:Carbon Radioisotopes; D020106:Acrylamide