title: Highly sensitive microplate-ELISA for angiotensin I using 3,3', 5,5'-tetramethylbenzidine.
abstract: A microplate-ELISA was established for angiotensin (Ang) I using Ang I-peroxidase conjugate, antiAng I antibodies which could distinguish Ang I from Ang II and Ang III, and 3,3', 5,5'-tetramethylbenzidine. Ang I could be quantified in amounts as low as 1 pg by the spectrophotometric method. The present assay system was the most sensitive and rapid in all Ang I assay systems described so far. The assay system was applicable to determination of Ang I concentration in human plasma and abdominal fluid.
authors.affiliation: Department of Biotechnology, Faculty of Agriculture, Gifu University, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Suzuki
authors.forename: S
authors.initials: S
authors.lastname: Yamashita
authors.forename: A
authors.initials: A
authors.lastname: Takahashi
authors.forename: M
authors.initials: M
authors.lastname: Ito
authors.forename: S
authors.initials: S
authors.lastname: Miyazaki
authors.forename: Y
authors.initials: Y
authors.lastname: Nagata
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
mesh_terms: D000328:Adult; D000803:Angiotensin I / Q000097:blood*; D001560:Benzidines; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008875:Middle Aged; D012084:Renin-Angiotensin System
chemical_list: D001560:Benzidines; C021758:3,3',5,5'-tetramethylbenzidine; D000803:Angiotensin I