Increased level of histone H1(0) messenger RNA in hypoxic Ehrlich ascites tumor cells.
We have investigated the expression of the H1 histone subtype H1(0) gene in Ehrlich ascites tumor cells (EAT) under varied conditions of oxygen supply. Our results show that proliferating EAT cells express H1(0) mRNA at a basal level under normoxic conditions. Severe hypoxia leads to a cessation of cell growth and causes an accumulation of cells in G1. Here, we show that the level of H1(0) histone mRNA increases within a few hours after the onset of hypoxia.
Bouterfa
H
H
Institute of Biochemistry, University of Göttingen, Federal Republic of Germany.
Doenecke
D
D
Löffler
M
M
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D005260:Female; D006657:Histones / Q000235:genetics* / Q000378:metabolism; D000860:Hypoxia / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D007399:Interphase / Q000502:physiology; D051379:Mice; D010100:Oxygen / Q000378:metabolism / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology
D006657:Histones; D012333:RNA, Messenger; D010100:Oxygen