The structural proteins of a porcine paramyxovirus (LPMV).
The porcine paramyxovirus is a newly identified agent of a fatal disease in piglets, endemic in Mexico since 1980, where it was seen around the town of La Piedad, Michoacan, Mexico (hence LPM virus). At least six [35S]methionine-labelled proteins could be resolved by SDS-PAGE and five of them were clearly immunoprecipitated. Selective labelling of LPMV-infected cells with [3H]glucosamine revealed two bands with an Mr of about 66K and 59K, corresponding to the two viral glycoproteins, the haemagglutinin-neuraminidase protein and the fusion protein. Labelling of virus with [32P]orthophosphate disclosed one band with an Mr of 52K, corresponding to the phosphoprotein. Analysis of nucleocapsids obtained from purified virus or from a permanently infected cell line revealed one major band with an Mr of 68K, the nucleoprotein. Two other proteins were also identified, the large protein and the matrix protein, with apparent Mr of about 200K and 40K, respectively. The protein migration pattern of LPMV was compared, by SDS-PAGE, with that of Newcastle disease virus, bovine parainfluenza 3 virus and Sendai virus. Differences in the Mr of LPMV proteins and the proteins of these paramyxoviruses were observed. We propose that LPMV should be classified as a novel member of the genus Paramyxovirus.
Sundqvist
A
A
Department of Veterinary Microbiology, BMC, Uppsala, Sweden.
Berg
M
M
Hernandez-Jauregui
P
P
Linné
T
T
Moreno-López
J
J
D000818:Animals; D001345:Autoradiography; D002213:Capsid / Q000302:isolation & purification / Q000648:ultrastructure; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D008715:Methionine / Q000378:metabolism; D008854:Microscopy, Electron; D008970:Molecular Weight; D010252:Paramyxoviridae / Q000032:analysis* / Q000254:growth & development; D013462:Sulfur Radioisotopes; D013552:Swine; D014758:Viral Core Proteins / Q000302:isolation & purification / Q000648:ultrastructure; D015678:Viral Structural Proteins / Q000096:biosynthesis / Q000302:isolation & purification*
D013462:Sulfur Radioisotopes; D014758:Viral Core Proteins; D015678:Viral Structural Proteins; D008715:Methionine