Neuromedin U-like immunoreactivity in rat intestine: regional distribution and immunohistochemical study.
Neuromedin U-25 and its C-terminal octapeptide, neuromedin U-8 are related peptides originally identified in porcine brain which elicit potent uterus stimulant activity. Radioimmunoassay using an antiserum raised against porcine neuromedin U-8, indicated that neuromedin U-like immunoreactivity in the rat was far more abundant in the small intestine than the brain. Neuromedin U in the rat is a single, a 23 amino acid peptide (2, 17). Rat neuromedin U has the same 7 residues on its C-terminus as porcine neuromedin U, and the antiserum against porcine neuromedin U-8 is 100% crossreactive with rat neuromedin U. Immunohistochemical analyses using this antiserum revealed that neuromedin U-immunoreactive structures in rat intestine were confined to the enteric nervous system, implying that neuromedin U may be involved in neuronal regulation of gut function.
Honzawa
M
M
Department of Nutrition and Food Science, Hyogo Women's College, Japan.
Sudoh
T
T
Minamino
N
N
Kangawa
K
K
Matsuo
H
H
D000595:Amino Acid Sequence; D000818:Animals; D003429:Cross Reactions; D007082:Ileum / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure; D007421:Intestine, Small / Q000276:immunology / Q000294:innervation / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D009479:Neuropeptides; C056900:neuromedin U