Tonic suppression of baroreceptor reflex by endogenous neurotensin in the rat.
We evaluated the modulatory role of endogenous neurotensin (NT) in baroreceptor reflex (BRR) response in Sprague-Dawley rats anesthetized with pentobarbital sodium. Intracerebroventricular (i.c.v.) administration of NT (15 or 30 nmol) significantly reduced the sensitivity of the BRR response. Blocking the endogenous activity of the tridecapeptide with its specific antagonist, (D-Trp11)-NT (4 or 8 nmol) or antiserum against NT (1:20); or inhibiting the aminopeptidases with bestatin (200 nmol), on the other hand, promoted a potentiation of BRR response. When administered together with bestatin (200 nmol), the suppressive effect of NT (15 nmol) on the BRR response was further enhanced, as was the augmentative action of (D-Trp11)-NT (4 nmol). Upon microinjection into the bilateral nucleus tractus solitarius (NTS), NT (600 pmol) and (D-Trp11)-NT (150 pmol) respectively elicited a reduction and enhancement of the BRR response. These results suggest that neurons that contain NT may participate in central cardiovascular regulation by tonically suppressing the BRR, possibly via an action on the NTS where baroreceptor afferents terminate.
Chen
C T
CT
Department of Institute of Pharmacology, National Yang-Ming Medical College, Taipei, Taiwan Republic of China.
Chan
J Y
JY
Barnes
C D
CD
Chan
S H
SH
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D006339:Heart Rate / Q000187:drug effects; D006973:Hypertension / Q000139:chemically induced; D007106:Immune Sera / Q000378:metabolism; D007276:Injections, Intraventricular; D007700:Kinetics; D007930:Leucine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D008297:Male; D009496:Neurotensin / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology*; D010656:Phenylephrine / Q000009:adverse effects; D011311:Pressoreceptors / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012018:Reflex / Q000187:drug effects*; D013997:Time Factors
D007106:Immune Sera; D010656:Phenylephrine; D009496:Neurotensin; C024309:neurotensin, Trp(11)-; D007930:Leucine; C012211:ubenimex