title: Clinical applications of flow karyotyping in myelocytic leukemia by stimulation of different subpopulations of cells in blood or bone marrow samples.
abstract: Examples are presented in which normal as well as abnormal chromosome distributions could be obtained from the same individual by means of bivariate flow karyotyping. Selective stimulation of T-lymphocytes obtained by E-rosetting from the blood of a patient with acute myelocytic leukemia resulted in a normal flow karyogram. The specific stimulation of myelocytic leukemia cells with granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin 3 (IL-3) yielded flow karyograms displaying the leukemia-associated chromosome abnormalities. The resulting flow karyograms could be used to discriminate between homolog differences, which appear normally in virtually every individual, and leukemia-associated chromosomal aberrations. In the case of a female chronic myelocytic leukemia patient who received bone marrow form an HLA-identical male donor, specific stimulation of various subsets of cells enabled to discriminate between leukemic host cells and non-leukemic donor cells. Both the leukemia-specific translocations and sex chromosomes were used as markers to analyse the flow karyograms obtained from the same sample.
authors.affiliation: Radiobiological Institute TNO, Rijswijk, The Netherlands.
authors.forename: G J
authors.initials: GJ
authors.lastname: Arkesteijn
authors.forename: H
authors.initials: H
authors.lastname: van Dekken
authors.forename: A C
authors.initials: AC
authors.lastname: Martens
authors.forename: A
authors.initials: A
authors.lastname: Hagenbeek
mesh_terms: D016026:Bone Marrow Transplantation; D002872:Chromosome Deletion; D015342:DNA Probes; D005260:Female; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006801:Humans; D007621:Karyotyping; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000473:pathology; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000473:pathology; D008297:Male; D009693:Nucleic Acid Hybridization; D010677:Philadelphia Chromosome; D012008:Recurrence; D014998:Y Chromosome / Q000032:analysis*
chemical_list: D015342:DNA Probes; D005456:Fluorescent Dyes