title: Purification and characterization of bioactive peptides from skin extracts of Rana esculenta.
abstract: The peptide fraction extracted by methanol from the skin of Rana esculenta, a species widely distributed in Western Europe, was investigated. The pharmacological activity found in the extract is attributable to the presence of authentic bradykinin, together with a shorter, partially active version of this molecule, des-Arg9-bradykinin. Also the bradykinin fragment 1-7 has been isolated, but it was inactive in our bioassay system. Moreover, a family of hydrophobic peptides has been purified and characterized, which appeared devoid of pharmacological activities when tested on smooth muscle preparations, but were provided with hemolytic activities.
authors.affiliation: Dipartimento di Scienze Biochimiche, Università La Sapienza, Rome, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Simmaco
authors.forename: D
authors.initials: D
authors.lastname: De Biase
authors.forename: C
authors.initials: C
authors.lastname: Severini
authors.forename: M
authors.initials: M
authors.lastname: Aita
authors.forename: G F
authors.initials: GF
authors.lastname: Erspamer
authors.forename: D
authors.initials: D
authors.lastname: Barra
authors.forename: F
authors.initials: F
authors.lastname: Bossa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001920:Bradykinin / Q000031:analogs & derivatives / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology; D011487:Protein Conformation; D011893:Rana esculenta / Q000378:metabolism*; D012867:Skin / Q000032:analysis*
chemical_list: D010455:Peptides; C063515:bioactive peptide A1, Rana esculenta; C063523:bioactive peptide B9, Rana esculenta; C024626:bradykinin, des-Arg(9)-; D001920:Bradykinin