Purification of antigen B from Taenia solium cysticerci by affinity to mammalian collagen.
A 1-step procedure for the easy and rapid purification of milligram amounts of antigen B from a crude extract of Taenia solium cysticerci is described here. This procedure takes advantage of the property of the antigen B to bind to collagen and is based on antigen adsorption to polymeric collagen.
Laclette
J P
JP
Department of Immunology, Instituto de Investigaciones Biomédicas, U.N.A.M., México, D.F.
Alagón
A
A
Willms
K
K
Torre-Blanco
A
A
D000327:Adsorption; D000818:Animals; D000947:Antigens, Helminth / Q000302:isolation & purification*; D015153:Blotting, Western; D002846:Chromatography, Affinity; D003094:Collagen / Q000378:metabolism*; D003552:Cysticercus / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D013552:Swine; D013621:Taenia / Q000276:immunology*
D000947:Antigens, Helminth; D003094:Collagen