The deoxyuridine suppression test in peripheral lymphocytes.
The effects of different deoxyuridine (dU) concentrations on lymphocytes stimulated with phytohemagglutinin (PHA) were studied for use in a lymphocyte dU suppression test (L-dUST). High concentrations of dU were necessary to overcome the unspecific spontaneous pattern of folate deficiency, dU suppression tests with cells of bone marrow (BM-dUST) and with lymphocytes were carried out in 15 patients with vitamin B-12 deficiency, in 12 with folate deficiency, and in 10 with other pathological conditions. L-dUST was also carried out in 15 healthy reference controls. The BM-dUST was able to distinguish patients with vitamin B-12 or folate acid deficiencies from those without, while the L-dUST was unable to do so in most cases. L-dUST does not, therefore, appear to be a reliable method for the diagnosis of megaloblastic changes.
Remacha
A
A
Servicio de Hematología, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
Barceló
M J
MJ
Pastor
M
M
Ubeda
J
J
Espadaler
M
M
Gimferrer
E
E
D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003857:Deoxyuridine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007457:Iodine Radioisotopes; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D010835:Phytohemagglutinins / Q000494:pharmacology; D014806:Vitamin B 12 Deficiency / Q000378:metabolism
D007457:Iodine Radioisotopes; D010835:Phytohemagglutinins; D003857:Deoxyuridine