High-performance liquid chromatography determination of erythrocyte membrane phospholipid composition in several animal species.
High-performance liquid chromatography (HPLC) was used to determine the phospholipid (PL) composition of ovine, equine, bovine, porcine, and canine RBC membranes. Procedural modifications of established techniques provided for separation of 7 PL within a 15- to 20-minute sample run. Significant (P less than 0.05) differences were detected in RBC membrane PL composition among the various species. The concern for physiologic properties associated with hemolysis and/or sedimentation rate must include evaluation of differences in the PL bilayer structure.
Engen
R L
RL
Department of Physiology and Pharmacology, College of Veterinary Medicine, Iowa State University, Ames 50011.
Clark
C L
CL
D000818:Animals; D002417:Cattle / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000662:veterinary*; D004285:Dogs / Q000097:blood; D004910:Erythrocyte Membrane / Q000032:analysis*; D006736:Horses / Q000097:blood; D010743:Phospholipids / Q000032:analysis*; D012756:Sheep / Q000097:blood; D013045:Species Specificity; D013552:Swine / Q000097:blood
D010743:Phospholipids