Sequence specificity of the deoxyribonuclease activity of 1,10-phenanthroline-copper ion.
A statistical analysis of a data set composed of over 1600 scission events of DNA produced by the 2:1 1,10-phenanthroline-copper complex (OP-Cu) has demonstrated that the nucleotide 5' to the site of phosphodiester bond scission is a primary influence in the kinetics of cleavage at any sequence position. The scission was less affected by the 3' neighbor. For each of the sixteen possible dinucleotides, a kinetic parameter can be computed reflecting scission at the 3' nucleotide. When used to predict the scission pattern of a DNA sequence not part of the present data set, correlation coefficients of about 0.6 between predicted and observed patterns were obtained.
Yoon
C
C
Department of Biological Chemistry, School of Medicine, University of California, Los Angeles 90024.
Kuwabara
M D
MD
Spassky
A
A
Sigman
D S
DS
D001483:Base Sequence; D003300:Copper; D003432:Cross-Linking Reagents; D004247:DNA; D003851:Deoxyribonucleases; D007364:Intercalating Agents; D007700:Kinetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010618:Phenanthrolines; D011685:Purine Nucleotides; D011742:Pyrimidine Nucleotides; D013698:Templates, Genetic
D003432:Cross-Linking Reagents; D007364:Intercalating Agents; D010618:Phenanthrolines; D011685:Purine Nucleotides; D011742:Pyrimidine Nucleotides; D003300:Copper; D004247:DNA; D003851:Deoxyribonucleases; C025205:1,10-phenanthroline