Cloning and sequence analysis of the human gene encoding eosinophil major basic protein.
Eosinophil granule major basic protein (MBP), a potent toxin for helminths and mammalian cells, is a single polypeptide rich in arginine. The gene, mbp, was cloned and its nucleotide sequence determined. The 3.3-kb gene consists of six exons and five introns, one of which contains an Alu family repeat. The combined exon sequence is similar to previously reported mbp cDNA sequences. The gene is immediately preceded by a putative promoter containing typical TATA and CCAAT boxes. Southern blots indicate that mbp exhibits limited polymorphism.
Barker
R L
RL
Department of Immunology, Mayo Medical School, Rochester, MN.
Loegering
D A
DA
Arakawa
K C
KC
Pease
L R
LR
Gleich
G J
GJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D001798:Blood Proteins / Q000235:genetics*; D015139:Blotting, Southern; D003001:Cloning, Molecular / Q000379:methods; D003062:Codon / Q000235:genetics; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D047091:Eosinophil Granule Proteins; D004804:Eosinophils / Q000378:metabolism; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012260:Ribonucleases
D001798:Blood Proteins; D003062:Codon; D047091:Eosinophil Granule Proteins; D004247:DNA; D012260:Ribonucleases