Automated assays for superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase activity.
Automated assays for catalase, glutathione peroxidase, glutathione reductase, and superoxide dismutase are presented. The assay for catalase is based on the peroxidatic activity of the enzyme. The glutathione peroxidase and reductase assays measure the consumption of NADPH following the reduction of t-butyl hydroperoxide and oxidized glutathione, respectively. The assay for superoxide dismutase is based on the reduction of cytochrome c. All assays utilize the Cobas FARA clinical automated analyzer and provide considerable time savings over the manual assays.
Wheeler
C R
CR
Division of Toxicology, Letterman Army Institute of Research, Presidio of San Francisco, California 94129-6800.
Salzman
J A
JA
Elsayed
N M
NM
Omaye
S T
ST
Korte
D W
DW
D000818:Animals; D001322:Autoanalysis; D002374:Catalase / Q000032:analysis*; D002417:Cattle; D005979:Glutathione Peroxidase / Q000032:analysis*; D005980:Glutathione Reductase / Q000032:analysis*; D051381:Rats; D013482:Superoxide Dismutase / Q000032:analysis*
D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase