Contribution of Cl(-)-OH- exchange to electroneutral NaCl absorption in rat distal colon.
Neutral NaCl absorption is the predominant Na+ absorptive process in rat distal colon. Whether this neutral NaCl absorptive process represents Na(+)-Cl- cotransport or dual ion exchanges of Na(+)-H+ and Cl(-)-OH- has been uncertain. Recent studies using rat colonic brush-border membrane vesicles (BBMVs) have described a Na(+)-H+ exchange mechanism and have proposed that net NaCl absorption occurs via a dual ion exchange process. To date, however, an anion exchanger on the colonic apical membrane has not been identified. To determine whether a Cl(-)-OH- exchange process is present, 36Cl uptake was evaluated across rat distal colonic BBMVs. A pH gradient (7.7 in/5.5 out) stimulated active Cl- uptake. Cl- uptake was not significantly affected by the presence of a valinomycin-induced K+ diffusion potential (inside positive), suggesting that a Cl- conductive pathway is not present in these membranes. The pH gradient-stimulated Cl- uptake was a saturable function of the Cl- concentration with a Km of 14.3 +/- 5.0 mM and a Vmax of 20.4 +/- 5.5 nmol.mg protein-1.30 s-1 and was almost completely inhibited by 1 mM concentrations of SITS and DIDS, inhibitors of anion exchange processes in other epithelia. Inward gradients of Na+, K+, or Na+ and K+ did not further stimulate initial Cl- uptake, suggesting that coupling of Na+ and Cl- movement does not occur by a cotransport mechanism. Thus a Cl(-)-OH- exchange process is present in rat distal colonic BBMVs.(ABSTRACT TRUNCATED AT 250 WORDS)
Foster
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Department of Medicine, University of Chicago Hospitals and Clinics, Pritzker School of Medicine, Illinois 60616.
Dudeja
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Brasitus
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D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism*; D003106:Colon / Q000502:physiology*; D006878:Hydroxides / Q000378:metabolism*; D007408:Intestinal Absorption; D007700:Kinetics; D008297:Male; D008871:Microvilli / Q000378:metabolism; D008954:Models, Biological; D009130:Muscle, Smooth / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D012965:Sodium Chloride / Q000378:metabolism*; D014634:Valinomycin / Q000494:pharmacology
D002712:Chlorides; D006878:Hydroxides; D014634:Valinomycin; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D012965:Sodium Chloride; C031356:hydroxide ion; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid