Design of a microprocessor-controlled thermoregulated chamber for perfusing in vitro mammalian brain preparations.
A system is described that allows to perfuse in vitro isolated mammalian brain preparations obtained from adult guinea-pigs. It consists of a microprocessor controlled thermoregulation unit, a perfusion line and a perfusion/recording chamber. The main features of the thermoregulation unit include the ability to maintain stable temperatures between 10 and 37 degrees C and to precisely control the time course of the temperature changes. Using this system, perfused tissue blocks can be kept viable for 8-10 h.
Mühlethaler
M
M
Département de Physiologie, CMU, Geneva, Switzerland.
Cand
P
P
Henauer
R
R
Serafin
M
M
D000818:Animals; D001923:Brain Chemistry; D006168:Guinea Pigs; D006358:Hot Temperature; D066298:In Vitro Techniques; D008838:Microcomputers; D010477:Perfusion / Q000295:instrumentation* / Q000379:methods