title: Role of skin in the burn-induced reduction of reticuloendothelial phagocytic activity in rats.
abstract: Acute burn trauma has been demonstrated to depress reticuloendothelial system (RES) phagocytic activity, which could partially explain the development of septicaemia in burn patients. In the present study we have attempted to determine the role that skin plays in the depression of the RES. One group of Lewis rats was subjected to a 100 degrees C scald burn for 10 s. Eschar was then removed and implanted onto the backs of a second group of normal controls. A third group of Lewis rats were subjected to sham treatment; the eschar of these animals was removed and implanted onto the back of a fourth group of normal rats. The excision sites of the donor animals were immediately covered with Biobrane. Twenty-four hours later the technetium-99m sulphur colloid [( 99mTS]SC) method described earlier (Trop et al., 1989) was used to determine phagocytosis in these four groups of animals. Acute burn trauma produced a marked reduction in colloid uptake by the spleen and a marked increase in the uptake of colloid material by the lung, although no effect was observed on liver or kidney uptake. Implantation of the burn eschar into normal control rats had no statistically significant effect upon colloid uptake. These data suggest that alterations in phagocytic activity of the spleen and lung occur within minutes after burn injury and may be unrelated to the presence of the burn eschar itself.
authors.affiliation: Department of Pediatrics, Harvard Medical School, Massachusetts General Hospital, Boston.
authors.forename: M
authors.initials: M
authors.lastname: Trop
authors.forename: E J
authors.initials: EJ
authors.lastname: Schiffrin
authors.forename: E A
authors.initials: EA
authors.lastname: Carter
mesh_terms: D000208:Acute Disease; D000818:Animals; D002056:Burns / Q000503:physiopathology*; D003102:Colloids; D005260:Female; D008168:Lung / Q000378:metabolism; D012157:Mononuclear Phagocyte System / Q000503:physiopathology*; D010587:Phagocytosis; D051381:Rats; D011917:Rats, Inbred Lew; D012867:Skin / Q000503:physiopathology*; D013154:Spleen / Q000378:metabolism; D013667:Technetium; D013997:Time Factors
chemical_list: D003102:Colloids; D013667:Technetium