Members of the alpha-amylase inhibitors family from wheat endosperm are major allergens associated with baker's asthma.
We have identified the major antigens or IgE binding components from wheat flour. Thirty-five sera from patients with baker's asthma were used to analyze the reaction with wheat salt-soluble proteins. We found a 15 kDa SDS-PAGE band which reacted with all sera tested. Purified members of the alpha-amylase inhibitor family, which are the main components of the 15 kDa band, were recognized by specific IgE when tested with a pool of reactive sera. Immunodetection after two-dimensional electrophoretic fractionation of crude inhibitor preparations from wheat endosperms also detected several inhibitor subunits as major low-molecular-weight allergens.
Gómez
L
L
Departamento de Bioquimica, ETS Ingenieros Agrónomos, Madrid, Spain.
Martín
E
E
Hernández
D
D
Sánchez-Monge
R
R
Barber
D
D
del Pozo
V
V
de Andrés
B
B
Armentia
A
A
Lahoz
C
C
Salcedo
G
G
D000485:Allergens / Q000032:analysis* / Q000276:immunology; D000941:Antigens / Q000032:analysis* / Q000276:immunology; D001249:Asthma / Q000276:immunology*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology; D008970:Molecular Weight; D014908:Triticum / Q000276:immunology*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*
D000485:Allergens; D000941:Antigens; D007106:Immune Sera; D007073:Immunoglobulin E; D000516:alpha-Amylases