An improved method for complement subcomponent C1R typing.
An improved method has been developed for the reliable classification of different C1R genetic variant forms from human serum or plasma. The method combines the use of neuraminidase-digested samples followed by monodimensional isoelectric focusing in the pH range 5 to 8 followed by immunoblotting. The method yields a simple pattern, with one major band in homozygote and two major bands in heterozygote cases.
Kamboh
M I
MI
Department of Human Genetics, Graduate School of Public Health, University of Pittsburgh, PA.
Ferrell
R E
RE
D001788:Blood Grouping and Crossmatching / Q000379:methods*; D015923:Complement C1r / Q000032:analysis*; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015151:Immunoblotting; D007525:Isoelectric Focusing; D009439:Neuraminidase / Q000378:metabolism; D010641:Phenotype
D009439:Neuraminidase; D015923:Complement C1r