title: NMR analysis of low-density lipoprotein oxidatively-modified in vitro.
abstract: Human plasma low density lipoprotein has been oxidized at different stages in vitro and analysed by 1H, 13C, and 31P NMR spectroscopy and by biochemical methods. Information was obtained on: a) structure mobilities of lipids and on lipid-protein interactions; b) conjugated and oxo-dienes; c) polyunsaturated/monounsaturated fatty acid chains; d) lysophosphatidylcholine production. The results show that the NMR approach is particularly useful for the assessment of structural modification in oxidized LDL.
authors.affiliation: Fondazione Rivetti, Laboratorio di Biochimica e Biologia Molecolare, Milano, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Barenghi
authors.forename: S
authors.initials: S
authors.lastname: Bradamante
authors.forename: G A
authors.initials: GA
authors.lastname: Giudici
authors.forename: C
authors.initials: C
authors.lastname: Vergani
mesh_terms: D003300:Copper; D005227:Fatty Acids / Q000378:metabolism; D005609:Free Radicals; D006801:Humans; D007501:Iron; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008244:Lysophosphatidylcholines / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010084:Oxidation-Reduction; D013858:Thiobarbiturates
chemical_list: D005227:Fatty Acids; D005609:Free Radicals; D008077:Lipoproteins, LDL; D008244:Lysophosphatidylcholines; D013858:Thiobarbiturates; D003300:Copper; D007501:Iron; C029684:thiobarbituric acid