title: The complete amino acid sequences of alpha subunits of chum salmon gonadotropins.
abstract: The complete amino acid sequences of alpha subunits of chum salmon (Oncorhynchus keta) gonadotropins (GTHs), GTH I and GTH II, were determined. Subunits were separated by reversed-phase high-performance liquid chromatography. Two alpha subunits, I alpha 1 and I alpha 2, were obtained from GTH I, whereas only one alpha subunit was obtained from GTH II. Sequence analysis revealed that GTH I alpha 2 is identical to GTH II alpha, but distinct from GTH I alpha 1. The I alpha 1 and II alpha consist of 95 and 92 amino acid residues, respectively. GTH I alpha 1 has 72% sequence identity with GTH I alpha 2. The two alpha subunits show approximately 65% sequence identity with bovine alpha subunit. This is the first finding of two chemically distinct alpha subunits from one species.
authors.affiliation: Laboratory of Molecular Endocrinology, School of Fisheries Sciences, Kitasato University, Iwate, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Itoh
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Kawauchi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006065:Gonadotropins, Pituitary / Q000302:isolation & purification; D008322:Mammals; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D012474:Salmon; D012689:Sequence Homology, Nucleic Acid
chemical_list: D006065:Gonadotropins, Pituitary