Differentiating effect of L-tyrosine on the human melanoma cell line IIB-MEL-J.
IIB-MEL-J is a highly heterogeneous newly established human melanoma cell line. The addition of 3 mM L-tyrosine to the culture medium produced (1) a great decrease in the cell growth rate, (2) a loss of the anchor-age-independent growth capacity, and (3) a change in the morphology of the cells to a fibroblastoid aspect. Coincident with these changes, an increase in subpopulations I and II and a decrease in subpopulations III and IV took place. In view of this evidence we consider that the cells have differentiated. The melanin production was not increased by the L-tyrosine treatment, suggesting that differentiation and melanin expression are not strictly correlated.
Guerra
L
L
Instituto de Investigaciones Bioquimicas Luis F. Leloir Fundacion Campomar, Buenos Aires, Argentina.
Bover
L
L
Mordoh
J
J
D002448:Cell Adhesion / Q000187:drug effects; D002469:Cell Separation; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002499:Centrifugation, Density Gradient; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D008543:Melanins / Q000378:metabolism; D008545:Melanoma / Q000378:metabolism / Q000473:pathology*; D008875:Middle Aged; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D014443:Tyrosine / Q000494:pharmacology*
D008543:Melanins; D014443:Tyrosine