Establishment from an adult leukemic patient of two novel precursor B cell lines with different growth modality.
Two novel cell lines, PC-53 and PC-53A were established from an adult ALL patient, at third relapse and in the terminal accelerated phase respectively. Both lines displayed the phenotype of B-cell precursors (CD19+, CD38+, CD20-, cytoplasmic-mu-, immunoglobulin gene rearrangement), identical to the freshly isolated blast cells. Chromosomal analysis showed a prominent 45-XX karyotype, including three marker chromosomes. No chromosome 8 abnormalities were detectable, consistently with a non-rearranged c-myc locus. Both cell lines were EBV-negative. Growth stimulation by autologous supernatant was observed for PC-53 cells during the first 4 months in culture, whereas it was much less evident for PC-53A cells. Thus, PC-53 and PC-53A cells represent a useful tool to investigate the mechanisms involved in the clonal expansion of B-cell precursors.
Tarella
C
C
Dipartimento di Medicina ed Oncologia Spermientale, Sezione di Ematologia, Torino, Italy.
Pregno
P
P
Gallo
E
E
Caracciolo
D
D
Stefani
S
S
Barletta
C
C
Lanfrancone
L
L
Ferrero
D
D
D000328:Adult; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D015139:Blotting, Southern; D002455:Cell Division; D002460:Cell Line; D005260:Female; D006801:Humans; D007621:Karyotyping; D009857:Oncogenes / Q000235:genetics; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood* / Q000235:genetics