[Enzymatic determination of glandular kallikrein in human pancreatic secretions].
A method for the enzymatic determination of tissue kallikrein (EC 3.4. 21.8) in human pancreatic juice using D-Val-cyclohexyl-Ala-Arg-4-nitroaniline as substrate as well as the reaction conditions optimized for 37 degrees C are described. The reaction is characterized by the following kinetic parameters, determined according to Hanes and Eisenthal, Cornish-Bowden: KM = 2.10(-5) mol/l; Vmax = 0.800 mumol/(l.s). The coefficient of intra-assay variation (N = 10) was determined to 2.54%. The detection limit of the assay was found to be 8.63 nmol/(l.s).
Weber
H
H
Institut für Pathologische Biochemie, Bereichs Medizin der Wilhelm-Pieck-Universität Rostock.
Dummler
W
W
Liebe
S
S
D004796:Clinical Enzyme Tests; D006801:Humans; D007610:Kallikreins / Q000032:analysis*; D010189:Pancreatic Juice / Q000032:analysis*
D007610:Kallikreins
Enzymatische Bestimmung von glandulärem Kallikrein im menschlichen Pankreassekret.