title: Metabolic control of the expression of mitochondrial D-beta-hydroxybutyrate dehydrogenase, a ketone body converting enzyme.
abstract: The effects of various metabolic conditions inducing an overproduction of ketone bodies in the rat were studied at different levels of D-beta-hydroxybutyrate dehydrogenase expression, i.e., enzymatic activity and protein content in purified mitochondria, and translational activity of isolated free cytosolic polysomes. The strongest variations were obtained in diabetes mellitus where the D-beta-hydroxybutyrate dehydrogenase expression is largely decreased. Insulin can reverse this strong effect. Modulation of liver enzyme activity and of enzyme content was observed under the other conditions tested, i.e., a decrease and an increase in starvation and hyperlipidemic conditions, respectively. A comparative study was carried out on the enzyme of extrahepatic tissues, i.e., heart, kidney and brain. The results indicate that the D-beta-hydroxybutyrate dehydrogenase function appears to be controlled, at least at the translational, post-translational and catalytic levels.
authors.affiliation: Laboratoire de Biochimie et Biologie Moléculaire, UA CNRS 531, Université de Franche-Comté, Besancon, France.
authors.forename: A
authors.initials: A
authors.lastname: Kante
authors.forename: M
authors.initials: M
authors.lastname: Cherkaoui Malki
authors.forename: C
authors.initials: C
authors.lastname: Coquard
authors.forename: N
authors.initials: N
authors.lastname: Latruffe
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology / Q000235:genetics; D006884:Hydroxybutyrate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D006949:Hyperlipidemias / Q000201:enzymology; D007657:Ketone Bodies / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000201:enzymology; D011132:Polyribosomes / Q000201:enzymology; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D013217:Starvation / Q000201:enzymology
chemical_list: D007657:Ketone Bodies; D006884:Hydroxybutyrate Dehydrogenase