Biophysical and biological activity of a synthetic 8.7-kDa hydrophobic pulmonary surfactant protein SP-B.
We have synthesized pulmonary surfactant apoprotein SP-B peptides by solid-phase chemistry and demonstrated their ability to enhance the surface-active properties of synthetic lipid mixtures. The synthetic peptides were reactive with antiserum generated against the native bovine surfactant peptide. Both peptides conferred surfactant-like properties to synthetic lipid mixtures as assessed by a Wilhelmy balance and pulsating bubble surfactometer. Likewise, mixtures of synthetic SP-B peptides and lipid restored compliance of isolated surfactant-deficient rat lungs. This work demonstrates the utility of SP-B as a functional component of pulmonary surfactant mixtures for treatment of respiratory distress syndrome or other disorders characterized by surfactant deficiency.
Sarin
V K
VK
Corporate Molecular Biology, Abbott Laboratories, Abbott Park, IL 60064.
Gupta
S
S
Leung
T K
TK
Taylor
V E
VE
Ohning
B L
BL
Whitsett
J A
JA
Fox
J L
JL
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006858:Hydrofluoric Acid; D007106:Immune Sera; D008168:Lung / Q000378:metabolism; D008969:Molecular Sequence Data; D011510:Proteolipids / Q000138:chemical synthesis* / Q000235:genetics / Q000502:physiology; D011663:Pulmonary Surfactants / Q000138:chemical synthesis* / Q000235:genetics / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D013500:Surface Tension
D007106:Immune Sera; D011510:Proteolipids; D011663:Pulmonary Surfactants; D012333:RNA, Messenger; D006858:Hydrofluoric Acid