Intraspecific sequence variation in the mitochondrial genome of rainbow trout (Oncorhynchus mykiss).
We examined the sequence of a 2214 base pair HindIII fragment from the mitochondrial genome of six rainbow trout. The fragment encodes four proteins and two tRNAs. Sequences for two fish from a single locality were identical. Those from separate localities differed by from 1 to 7 nucleotide substitutions. Of 13 variable sites, 12 were synonymous and 1 led to a conservative amino acid substitution. Transitions accounted for 12 of the 13 variants. In contrast to interspecific comparisons (Thomas and Beckenbach. 1989. J. Mol. Evol. 29: 233-245), the intraspecific divergence estimates based on sequence are less than those estimated from restriction fragment analysis, suggesting a complex, dynamic process for the accumulation of variation in the mitochondrial genome.
Beckenbach
A T
AT
Department of Biological Sciences, Simon Fraser University, Burnaby, B.C., Canada.
Thomas
W K
WK
Sohrabi
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003062:Codon; D004272:DNA, Mitochondrial / Q000235:genetics*; D005091:Exons; D014644:Genetic Variation; D008969:Molecular Sequence Data; D012487:Salmonidae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014337:Trout / Q000235:genetics*
D003062:Codon; D004272:DNA, Mitochondrial