title: Radioimmunoassay of nivalenol in barley.
abstract: Antibodies against nivalenol (NIV) tetraacetate (Tetra-Ac-NIV) were prepared by immunizing rabbits with a hemisuccinate derivative of 8-hydroxy-3,4,7,15-tetraacetyl-12, 13-epoxytrichothece-9-en conjugated to bovine serum albumin. A radioimmunoassay system with one of these sera was developed to measure NIV contamination in barley. The detection limit for Tetra-Ac-NIV was about 0.5 ng/ml. The relative cross-reactivities of the antiserum with Tetra-Ac-NIV, acetyl T-2 toxin, and scirpenol triacetate, which were determined by the competitive radioimmunoassay, were 1, 0.78, and 0.56, respectively. Other derivatives showed no cross-reactivity. For the determination of NIV in a barley sample, NIV was extracted from the sample with acetonitrile-water (7:3), defatted with hexane, and then acetylated with acetic anhydride to form Tetra-Ac-NIV. The reaction mixture was loaded onto a C18 cartridge to remove excess reagents and impurities. Tetra-Ac-NIV was eluted from the cartridge with 50% methanol in water, and the eluate was subjected to radioimmunoassay. Analysis of six naturally contaminated barley samples for NIV revealed that radioimmunoassay results agreed well with gas chromatographic analyses.
authors.affiliation: Division of Biochemistry and Immunochemistry, National Institute of Hygienic Sciences, Tokyo, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Teshima
authors.forename: K
authors.initials: K
authors.lastname: Hirai
authors.forename: M
authors.initials: M
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Ikebuchi
authors.forename: M
authors.initials: M
authors.lastname: Ichinoe
authors.forename: T
authors.initials: T
authors.lastname: Terao
mesh_terms: D002849:Chromatography, Gas; D002523:Edible Grain / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D005670:Fusarium / Q000302:isolation & purification; D001467:Hordeum / Q000032:analysis* / Q000382:microbiology; D009183:Mycotoxins / Q000032:analysis; D011863:Radioimmunoassay / Q000379:methods*; D012717:Sesquiterpenes / Q000032:analysis*; D014255:Trichothecenes / Q000032:analysis*
chemical_list: D009183:Mycotoxins; D012717:Sesquiterpenes; D014255:Trichothecenes; C038405:nivalenol