title: Immunophenotyping of acute leukemias using paraffin-embedded tissue sections.
abstract: In a study of 55 patients with either acute lymphoid leukemia (ALL; 25 cases) or acute myeloid leukemia (AML; 30 cases), paraffin-embedded bone marrow particle sections were examined with a panel of monoclonal and polyclonal antibodies reactive toward lymphoid and myeloid-associated antigens, using the alkaline phosphatase-anti-alkaline phosphatase (APAAP) technique. All cases were previously classified according to the French-American-British (FAB) Co-operative Group, and cases of ALL were immunophenotyped by flow cytometry. Results indicated that myeloid-associated antibodies (Mac 387, KP 1 [CD68], antielastase, antilactoferrin, and antilysozyme) did not react with any case of ALL, M1-AML, or M6-AML, whereas at least one of these antibodies reacted with 20 of 21 (95%) cases of M2, M3, M4, and M5-AML. Anti-glycophorin C marked cases of M6-AML, whereas anti-CD3 labeled T-cell ALL. None of the antibodies tested specifically identified cases of B-cell ALL. The authors conclude that use of a selected panel of antibodies on paraffin-embedded bone marrow particle sections may be of value in the diagnosis and immunophenotypic classification of many cases of acute leukemias.
authors.affiliation: Department of Pathology, State University of New York Health Science Center, Syracuse 13210.
authors.forename: A S
authors.initials: AS
authors.lastname: Kurec
authors.forename: V E
authors.initials: VE
authors.lastname: Cruz
authors.forename: D
authors.initials: D
authors.lastname: Barrett
authors.forename: D Y
authors.initials: DY
authors.lastname: Mason
authors.forename: F R
authors.initials: FR
authors.lastname: Davey
mesh_terms: D000208:Acute Disease; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis; D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism / Q000473:pathology*; D007951:Leukemia, Myeloid / Q000378:metabolism / Q000473:pathology*; D015458:Leukemia, T-Cell / Q000378:metabolism / Q000473:pathology*; D010641:Phenotype; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000378:metabolism / Q000473:pathology*; D012189:Retrospective Studies
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm