title: Dissolution of calcium bilirubinate and calcium carbonate debris remaining after methyl tert-butyl ether dissolution of cholesterol gallstones.
abstract: Methyl tert-butyl ether rapidly dissolves cholesterol gallstones, although insoluble debris may remain. Total gallstone dissolution could be achieved if safe solvents for these noncholesterol components can be developed. We evaluated the in vitro ability of ethylenediaminetetraacetic acid, citrate, dimethyl sulfoxide, and ionic or nonionic detergents to dissolve the predominantly calcium bilirubinate and calcium carbonate debris remaining after methyl tert-butyl ether gallstone dissolution. Ethylenediaminetetraacetic acid 1% or 2% at pH 9.5 was the most effective of the solvents studied for dissolving calcium and bile pigment. The addition of cholate (25-200 mM) or polysorbate (1%-10%) to ethylenediaminetetraacetic acid 1% at pH 9.5 enhanced pigment dissolution compared to ethylenediaminetetraacetic acid alone. Dissolution of pellets prepared from human gallstones and composed predominantly of either calcium bilirubinate or calcium carbonate was 80% and 85% at 4 h using ethylenediaminetetraacetic acid 1% plus polysorbate-20 1% at pH 9.5. We conclude that ethylenediaminetetraacetic acid, either alone or with a detergent, is an effective solvent for methyl tert-butyl ether-insoluble gallstone debris and deserves assessment in vivo.
authors.affiliation: Department of Gastroenterology, Mayo Clinic, Rochester, Minnesota.
authors.forename: P E
authors.initials: PE
authors.lastname: Nelson
authors.forename: T P
authors.initials: TP
authors.lastname: Moyer
authors.forename: J L
authors.initials: JL
authors.lastname: Thistle
mesh_terms: D001663:Bilirubin / Q000032:analysis*; D002119:Calcium Carbonate / Q000032:analysis*; D002769:Cholelithiasis / Q000032:analysis / Q000188:drug therapy*; D002784:Cholesterol / Q000378:metabolism*; D004353:Drug Evaluation, Preclinical; D004359:Drug Therapy, Combination; D004987:Ethers / Q000627:therapeutic use*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008738:Methyl Ethers; D012995:Solubility; D012997:Solvents / Q000627:therapeutic use*; D013055:Spectrophotometry, Infrared
chemical_list: D004987:Ethers; D008738:Methyl Ethers; D012997:Solvents; C043243:methyl tert-butyl ether; D002784:Cholesterol; D002119:Calcium Carbonate; D001663:Bilirubin