Effects of ethidium bromide treatment of mouse cells on expression and assembly of nuclear-coded subunits of complexes involved in the oxidative phosphorylation.
Using mouse cell lines 5P and 5PEr (ethidium bromide-resistant derivative of 5P), we examined the influence of blocking expression of mitochondrial-gene products with ethidium bromide on the expression and assemblies of nuclear-coded subunits of the complexes involved in the oxidative phosphorylation. The results suggest that in the absence of mitochondrial-coded products, the expressions of subunit VIc of complex IV and beta-subunit of F1-ATPase are not affected, but that most nuclear-coded subunits other than alpha- and beta-subunits of F1-ATPase cannot be assembled nor inserted into the inner membrane.
Hayashi
J
J
Department of Biochemistry, Saitama Cancer Center Research Institute, Saitama, Japan.
Tanaka
M
M
Sato
W
W
Ozawa
T
T
Yonekawa
H
H
Kagawa
Y
Y
Ohta
S
S
D000199:Actins / Q000235:genetics; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002478:Cells, Cultured; D004272:DNA, Mitochondrial / Q000187:drug effects / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D004996:Ethidium / Q000494:pharmacology*; D015870:Gene Expression; D051379:Mice; D008809:Mice, Inbred C3H; D008928:Mitochondria / Q000187:drug effects; D010085:Oxidative Phosphorylation
D000199:Actins; D004272:DNA, Mitochondrial; D004996:Ethidium