Evaluation of genotoxic effects of the herbicide dicamba using in vivo and in vitro test systems.
The genotoxic effects of the herbicide dicamba have been studied by measuring 1) the unwinding rate of liver DNA from intraperitoneally (i.p.) treated rats (fluorimetric assay); 2) DNA repair as unscheduled DNA synthesis (UDS) induced in cultured human peripheral blood lymphocytes (HPBL); and 3) sister chromatid exchanges (SCE) in HPBL. Results show that dicamba is capable of inducing DNA damage since it significantly increases the unwinding rate of rat liver DNA in vivo and also induces UDS in HPBL in vitro in the presence of exogenous metabolic activation (S-9 mix). Furthermore, dicamba causes a very slight increase in SCE frequency in HPBL in vitro.
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Centro Interuniversitario per la Ricerca sul Cancro, Università di Bologna, Italy.
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Rani
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Valenti
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AM
Mazzullo
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Colacci
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A
Grilli
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D000818:Animals; D001565:Benzoates / Q000494:pharmacology*; D002478:Cells, Cultured; D004249:DNA Damage; D004260:DNA Repair; D004277:DNA, Single-Stranded / Q000096:biosynthesis / Q000187:drug effects; D003996:Dicamba / Q000494:pharmacology*; D005069:Evaluation Studies as Topic; D006801:Humans; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009691:Nucleic Acid Denaturation; D051381:Rats; D011919:Rats, Inbred Strains; D012854:Sister Chromatid Exchange / Q000187:drug effects
D001565:Benzoates; D004277:DNA, Single-Stranded; D003996:Dicamba