title: Inositol is a constituent of detergent-solubilized immunoaffinity-purified rat liver 5'-nucleotidase.
abstract: myo-Inositol analysis of detergent-solubilized immunoaffinity-purified rat liver 5'-nucleotidase showed the presence of 1 mol of myo-inositol/mol of enzyme monomer. This provides unequivocal evidence that the ectoenzyme 5'-nucleotidase is attached to liver membranes by a glycosyl-phosphatidylinositol lipid anchor.
authors.affiliation: Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital, U.K.
authors.forename: E M
authors.initials: EM
authors.lastname: Bailyes
authors.forename: M A
authors.initials: MA
authors.lastname: Ferguson
authors.forename: C A
authors.initials: CA
authors.lastname: Colaco
authors.forename: J P
authors.initials: JP
authors.lastname: Luzio
mesh_terms: D015720:5'-Nucleotidase / Q000032:analysis* / Q000302:isolation & purification; D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry; D007294:Inositol / Q000032:analysis*; D008099:Liver / Q000201:enzymology*; D051381:Rats
chemical_list: D007294:Inositol; D015720:5'-Nucleotidase