The HIV protein, GP120, activates nuclear protein kinase C in nuclei from lymphocytes and brain.
Nuclear pool(s) of protein kinase C (PKC) may be a common target for hormones and growth factors which affect the trophic state of cells. The data presented demonstrate a time and dose-dependent activation of nuclear PKC by the HIV coat protein, gp120, in isolated nuclei from rat spleen and hippocampus. This gp120-stimulated PKC response was blocked by specific PKC inhibitors, a monoclonal antibody to PKC, and a monoclonal antibody directed against the murine T4 analog, L3T4. It is suggested that the gp120 interaction with the nuclear trophic factor-PKC system may impair normal gene expression, and thus result in the clinical symptoms associated in AIDS infection.
Zorn
N E
NE
Department of Pharmacology and Therapeutics, University of South Florida College of Medicine, Tampa 33612.
Weill
C L
CL
Russell
D H
DH
D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D000911:Antibodies, Monoclonal; D002467:Cell Nucleus / Q000201:enzymology*; D004789:Enzyme Activation; D015699:HIV Envelope Protein gp120 / Q000494:pharmacology*; D006624:Hippocampus / Q000201:enzymology*; D007700:Kinetics; D008214:Lymphocytes / Q000201:enzymology*; D008297:Male; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013110:Sphingosine / Q000494:pharmacology; D013154:Spleen / Q000201:enzymology; D019311:Staurosporine
D000470:Alkaloids; D000911:Antibodies, Monoclonal; D015699:HIV Envelope Protein gp120; D011493:Protein Kinase C; D019311:Staurosporine; D013110:Sphingosine