Chromosome mapping using polymerase chain reaction on somatic cell hybrids.
DNA polymerase chain reaction (PCR) was used to detect specific human sequences in human-mouse somatic hybrids. As compared with classic Southern blotting, this technique appeared to be more sensitive and less time-consuming for gene mapping.
Zhang
J
J
Center for Human Genetics, University of Leuven, Belgium.
Devriendt
K
K
Marynen
P
P
Van den Berghe
H
H
Cassiman
J J
JJ
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D002874:Chromosome Mapping; D005784:Gene Amplification; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011257:Pregnancy Proteins / Q000235:genetics
D011257:Pregnancy Proteins