Putrescine N-acetyltransferase in Onchocerca volvulus and Ascaris suum, an enzyme which is involved in polyamine degradation and release of N-acetylputrescine.
A novel type of N-acetyltransferase, clearly different from the nuclear and cytosolic polyamine N-acetyltransferases of mammals, was recently found in the intestinal nematode Ascaris suum. The occurrence of this putrescine N-acetylating enzyme has also been noted in the filarial parasite Onchocerca volvulus. The enzyme was partially purified from adults of O. volvulus and A. suum by chromatography on DEAE-cellulose and cadaverine-Sepharose. Substrate specificities of the filarial enzyme resemble those of the N-acetyltransferase from A. suum, with respect to its preference for putrescine and other diamines above polyamines and histones. Additionally, both nematode enzymes acetylated histamine, whereas dopamine and serotonin were not accepted as substrates. The activities of the N-acetyltransferase from O. volvulus and A. suum were potently inhibited by the drug berenil; the type of inhibition was competitive with respect to putrescine. The inhibition constants for berenil were determined as 4.2 and 1.2 microM for the enzymes of O. volvulus and A. suum, the Km values for putrescine were found to be 330 microM and 250 microM, respectively. Putrescine N-acetyltransferase is discussed as a regulatory step in the degradation of excessive polyamines via polyamine oxidase to putrescine. At this branching point, putrescine is retained in the cell for de novo synthesis of spermidine and spermine, catabolized via diamine oxidase or acetylated to a suitable transport product for excretion.
Wittich
R M
RM
Department of Biochemistry, Bernhard Nocht Institute of Tropical Medicine, Hamburg, F.R.G.
Walter
R D
RD
D000123:Acetyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D001200:Ascaris / Q000201:enzymology*; D015317:Biogenic Polyamines / Q000378:metabolism*; D002846:Chromatography, Affinity; D005260:Female; D009854:Onchocerca / Q000201:enzymology*; D015800:Protozoan Proteins / Q000302:isolation & purification / Q000378:metabolism; D011700:Putrescine / Q000031:analogs & derivatives* / Q000378:metabolism; D013379:Substrate Specificity; D000099228:Diamine N-Acetyltransferase
D000123:Acetyltransferases; D015317:Biogenic Polyamines; D015800:Protozoan Proteins; D011700:Putrescine; D000099228:Diamine N-Acetyltransferase; C026212:N-acetylputrescine