Osmotic and motional properties of intracellular water as influenced by osmotic swelling and shrinkage of Xenopus oocytes.
Experiments were done on fully grown Xenopus oocytes to determine the extent and the properties of cellular water of hydration. The studies involved the osmotic shrinking and swelling of the oocytes under known osmotic pressure as well as proton NMR spectral, titration, and free induction decay analyses. Studies were done both on whole oocytes and on subcellular fractions. The results show that little if any of the oocyte water in situ has the motional or osmotic properties expected of pure "bulk" water. Four distinct water of hydration compartments were found and defined on the basis of distinct hydrogen bounding mechanisms. Some of the water in yolk platelets was found not to be in fast exchange with other water compartments. Osmotic shrinkage of oocytes caused an adaptive decrease in the bound water of hydration compartments. This osmotically induced decrease is attributed to decreased surface area available for the hydrogen bounding of water molecules on cellular proteins.
Cameron
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IL
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284.
Merta
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P
Fullerton
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GD
D000818:Animals; D001826:Body Fluids / Q000502:physiology*; D002451:Cell Compartmentation; D003593:Cytoplasm / Q000502:physiology; D007424:Intracellular Fluid / Q000502:physiology*; D009682:Magnetic Resonance Spectroscopy; D009865:Oocytes / Q000502:physiology*; D013347:Subcellular Fractions; D014882:Water-Electrolyte Balance; D014982:Xenopus laevis / Q000502:physiology