Inhibitory effect of tumor necrosis factor on gene expression of hormone sensitive lipase in 3T3-L1 adipocytes.
Recombinant human tumor necrosis factor (TNF) depressed the activities of both lipoprotein lipase (LPL) and hormone sensitive lipase (HSL) in 3T3-L1 adipocytes, 3 to 24 h after its introduction to the cells. HSL gene expression, as measured by Northern blotting analysis with 32P-labeled cloned HSL-cDNA, was also suppressed. These results suggested that the reduction in HSL activity caused by TNF resulted from inhibited gene expression of the enzyme.
Sumida
M
M
Second Department of Medical Biochemistry, School of Medicine, Ehime University.
Sekiya
K
K
Okuda
H
H
Tanaka
Y
Y
Shiosaka
T
T
D000273:Adipose Tissue / Q000166:cytology / Q000201:enzymology*; D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis*; D002460:Cell Line; D015870:Gene Expression / Q000187:drug effects*; D051379:Mice; D012333:RNA, Messenger / Q000187:drug effects; D002787:Sterol Esterase / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D002265:Carboxylic Ester Hydrolases; D002787:Sterol Esterase