Optimization of an isocratic reversed phase liquid chromatographic system for the separation of fourteen steroids using factorial design and computer simulation.
An optimization strategy for an isocratic reversed phase high performance liquid chromatographic system (RP-HPLC) is described. Factorial design and a computer program are used to predict the retention time and resolution of fourteen steroids. An optimized rapid (less than 25 min) isocratic RP-HPLC system for the satisfactory separation of cortisone, cortisol, corticosterone, 11-deoxycortisol, 11-deoxycorticosterone, 17 alpha-hydroxyprogesterone, progesterone, androstenedione, testosterone, estrone, estradiol, estriol, prednisone acetate and dexamethasone acetate has been developed using this strategy through eight experiments.
Wei
J Q
JQ
Department of Internal Medicine, Affiliated Hospital of Shandong Medical University, Jinan, China.
Wei
J L
JL
Zhou
X T
XT
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003198:Computer Simulation; D006801:Humans; D008432:Mathematical Computing; D013256:Steroids / Q000032:analysis*
D013256:Steroids