Binding of low-density lipoproteins from chicken egg yolk to mouse anti-phospholipid B cells which include B cells against bromelain-treated mouse erythrocytes.
Low-density lipoproteins from chicken egg yolk (EyLDL), which are reactive with mouse antibodies against bromelain-treated mouse erythrocytes (BrMRBC), were conjugated with fluorescein isothiocyanate (FITC). FITC-EyLDL could bind specifically to mouse anti-phospholipid B cells, which comprised all the BrMRBC-rosette-forming cells and anti-BrMRBC lipopolysaccharide-reactive B cells, C3H/He mice at 12 weeks of age had, approximately, 7 x 10(5) EyLDL-binding cells in the peritoneal cavity, 3 x 10(5) EyLDL-binding cells in the pleural cavity, and 3 x 10(5) EyLDL-binding cells in the spleen. In ontogeny, the numbers of EyLDL-binding cells in the peritoneal cavity expanded greatly by 4 weeks. Other normal strains of mice and C3H/HeJ mice at 12 weeks of age had 4-7 x 10(5) EyLDL-binding cells in the peritoneal cavity; the numbers were large (19 x 10(5] in NZB mice, rather small (2 x 10(5] in MRL/lpr mice, and very small (0.1 x 10(5] in CBA/N mice. In some of various strains of mice at 12 months of age, more than 20% of peritoneal cells were EyLDL-binding cells; in particular, all of five older NZB mice examined had more than 10(7) EyLDL-binding cells in the peritoneal cavity.
Kawaguchi
S
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Department of Microbiology and Immunology, Shimane Medical University, Izumo, Japan.
D000367:Age Factors; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D001963:Bromelains / Q000494:pharmacology; D002645:Chickens; D004530:Egg Yolk; D004912:Erythrocytes / Q000187:drug effects / Q000276:immunology*; D005434:Flow Cytometry; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D008077:Lipoproteins, LDL / Q000276:immunology*; D008221:Lymphoid Tissue / Q000276:immunology; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology; D010743:Phospholipids / Q000276:immunology*
D007130:Immunoglobulin Idiotypes; D008077:Lipoproteins, LDL; D010743:Phospholipids; D001963:Bromelains