Latency to enter a mirrored chamber: a novel behavioral assay for anxiolytic agents.
Many animal species exhibit approach-avoidance responses upon the novel placement of a mirror into an individual animal's environment. With a view toward identifying new behavioral measures with qualitatively or quantitatively different responses to anxiolytic agents, we developed a mirrored chamber apparatus for which adult male BALB/cByJ mice showed an extended latency to enter. Administration of diazepam significantly reduced this latency to enter a mirrored chamber in a dosage-dependent manner. The psychomotor stimulant, methylphenidate, had no effect on latency to enter the mirrored chamber at a dose which stimulated locomotor activity to the same extent as diazepam. Thus, the decreased latency to enter the mirrored chamber brought about by diazepam seems unlikely to reflect the motor effects of this benzodiazepine. The potency of diazepam was significantly lower in the mirrored chamber assay than it was on three other measures of exploratory activity--"head-dipping" performance, plus-maze performance and locomotor activity stimulation. The findings of our study indicate that the mirrored chamber method is simple to carry out, nonpunishing, rapid and quantitative and that it possesses pharmacological attributes which distinguish its response to anxiolytics from other assays of exploratory behavior.
Toubas
P L
PL
Department of Pediatrics University of Oklahoma Health Sciences Center, Oklahoma City 73190.
Abla
K A
KA
Cao
W
W
Logan
L G
LG
Seale
T W
TW
D000818:Animals; D001007:Anxiety / Q000523:psychology*; D003220:Conflict, Psychological; D003975:Diazepam / Q000494:pharmacology*; D005106:Exploratory Behavior / Q000187:drug effects*; D008297:Male; D008774:Methylphenidate / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D009043:Motor Activity / Q000187:drug effects; D011930:Reaction Time / Q000187:drug effects*
D008774:Methylphenidate; D003975:Diazepam