An intercellular adhesion protein of rat testis: isolation and preliminary characterization.
By using biochemical and immunochemical approaches, we have isolated a rat spermatocyte surface protein of an apparent molecular weight of 80 kd involved in spermatocyte-Sertoli cell recognition in culture. Preliminary characterization of such a molecule has demonstrated that 1) it is a nonintegral membrane component; 2) it possesses a small amount of N-linked oligosaccharides of the high mannose type; and c) it is a stage-specific molecule, being present only in middle-late pachytene spermatocytes.
D'Agostino
A
A
Institute of Histology and General Embryology, University La Sapienza, Rome, Italy.
Stefanini
M
M
D000818:Animals; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000302:isolation & purification*; D006023:Glycoproteins / Q000302:isolation & purification; D008297:Male; D008540:Meiosis; D051381:Rats; D011919:Rats, Inbred Strains; D012708:Sertoli Cells / Q000502:physiology; D013090:Spermatocytes / Q000032:analysis* / Q000502:physiology; D013091:Spermatogenesis; D013737:Testis / Q000032:analysis* / Q000166:cytology
D015815:Cell Adhesion Molecules; D006023:Glycoproteins