NMR analysis of low-density lipoprotein oxidatively-modified in vitro.
Human plasma low density lipoprotein has been oxidized at different stages in vitro and analysed by 1H, 13C, and 31P NMR spectroscopy and by biochemical methods. Information was obtained on: a) structure mobilities of lipids and on lipid-protein interactions; b) conjugated and oxo-dienes; c) polyunsaturated/monounsaturated fatty acid chains; d) lysophosphatidylcholine production. The results show that the NMR approach is particularly useful for the assessment of structural modification in oxidized LDL.
Barenghi
L
L
Fondazione Rivetti, Laboratorio di Biochimica e Biologia Molecolare, Milano, Italy.
Bradamante
S
S
Giudici
G A
GA
Vergani
C
C
D003300:Copper; D005227:Fatty Acids / Q000378:metabolism; D005609:Free Radicals; D006801:Humans; D007501:Iron; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008244:Lysophosphatidylcholines / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010084:Oxidation-Reduction; D013858:Thiobarbiturates
D005227:Fatty Acids; D005609:Free Radicals; D008077:Lipoproteins, LDL; D008244:Lysophosphatidylcholines; D013858:Thiobarbiturates; D003300:Copper; D007501:Iron; C029684:thiobarbituric acid