trans activation of gene expression by v-myb.
The v-myb oncogene causes acute myelomonocytic leukemia in chickens and transforms avian myeloid cells in vitro. Its product, p48v-myb, is a short-lived nuclear protein which binds DNA. We demonstrate that p48v-myb can function as a trans activator of gene expression in transient DNA transfection assays. trans activation requires the highly conserved amino-terminal DNA-binding domain and the less highly conserved carboxyl-terminal domain of p48v-myb, both of which are required for transformation. Multiple copies of a consensus sequence for DNA binding by p48v-myb inserted upstream of a herpes simplex virus thymidine kinase promoter are strongly stimulatory for transcriptional activation by a v-myb-VP16 fusion protein but not by p48v-myb itself, suggesting that the binding of p48v-myb to DNA may not be sufficient for trans activation.
Ibanez
C E
CE
Department of Pathology, University of California, San Diego School of Medicine, La Jolla 92093.
Lipsick
J S
JS
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D003370:Coturnix; D004274:DNA, Recombinant; D004268:DNA-Binding Proteins / Q000502:physiology*; D005786:Gene Expression Regulation; D066298:In Vitro Techniques; D020626:Oncogene Proteins v-myb; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D009857:Oncogenes; D012045:Regulatory Sequences, Nucleic Acid; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D004268:DNA-Binding Proteins; D020626:Oncogene Proteins v-myb; D009856:Oncogene Proteins, Viral; D015682:Retroviridae Proteins, Oncogenic; D014157:Transcription Factors