Immunodetection with a monoclonal antibody of glutathione S-transferase mu in patients with and without carcinomas.
Several monoclonal antibodies against human liver glutathione S-transferase mu were developed. One of these monoclonal antibodies, called GST-3H4 was further characterized and used in this study. In hepatic tissue, after immunoblotting, GST-3H4 strains a 27 kDa protein with a pI value of 6.2. GST-3H4 recognizes other human class-mu glutathione S-transferases, but does not detect acidic or basic glutathione S-transferases. By immunodetection with this monoclonal antibody, glutathione S-transferase mu can be demonstrated in human breast, stomach, liver, small and large intestine, mononuclear blood cells, kidney and placenta. A 100% correlation is found in the distribution of glutathione S-transferase mu when different tissues or mononuclear blood cells from the same individuals are investigated. In 62.5% of the mononuclear blood cells from controls, glutathione S-transferase mu is present. In patients with polyposis coli, breast cancer or colon cancer a similar distribution is found. Therefore no important role for glutathione S-transferase mu deficiencies in the aetiology of these diseases is suggested.
Peters
W H
WH
Division of Gastrointestinal and Liver Diseases, St Radboud University Hospital, Nijmegen, The Netherlands.
Kock
L
L
Nagengast
F M
FM
Roelofs
H M
HM
D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000201:enzymology; D003110:Colonic Neoplasms / Q000201:enzymology; D005260:Female; D005982:Glutathione Transferase / Q000032:analysis*; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology; D007422:Intestines / Q000201:enzymology; D007527:Isoenzymes / Q000032:analysis*; D007963:Leukocytes, Mononuclear / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D009369:Neoplasms / Q000201:enzymology*; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D005982:Glutathione Transferase