[Experimental study of the viability of warm ischemic kidney. Relationship between ATP value in renal tissue by isotachophoresis and serum pyruvic acid to lactic acid ratio (P/L ratio)].
The viability of preserved kidney is considered to be evaluated by mitochondrial function. We used the three experimental conditions of WIT 60 minutes (group I), 90 minutes (group II), and 120 minutes (group III) using rabbit. We measured the ATP value of renal tissue as a direct method before ischemia (control), immediately before recirculation, and at one hour, two hours, one day, two days, four days and seven days after recirculation, using isotachophoresis (Tachophor LKB 2127), and as an indirect method, serum pyruvic acid, lactic acid and LDH values were measured simultaneously. The ATP value in groups I, II, and III before recirculation were 1/10 lower than controls. However, as WIT became longer, the recovery speed was diminished. The serum P/L ratio reflected the mitochondrial function in group III. (p less than 0.05) The measurement of ATP value by this method, although it is somewhat invasive, is a useful method with a simple technique and a short analysis time. The serum P/L ratio is considered not to reflect the mitochondrial function directly, but can be measured adequately in WIT 120 minutes.
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Department of Urology, Nagasaki University School of Medicine, Japan.
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D004586:Electrophoresis; D007511:Ischemia / Q000503:physiopathology; D007668:Kidney / Q000098:blood supply / Q000201:enzymology / Q000502:physiology*; D007773:Lactates / Q000097:blood*; D019344:Lactic Acid; D008928:Mitochondria / Q000502:physiology; D011773:Pyruvates / Q000097:blood*; D019289:Pyruvic Acid; D011817:Rabbits; D013696:Temperature; D014021:Tissue Preservation; D014022:Tissue Survival / Q000502:physiology*
D007773:Lactates; D011773:Pyruvates; D019344:Lactic Acid; D019289:Pyruvic Acid; D000255:Adenosine Triphosphate