Failure of platelet-activating factor (PAF-acether) to induce decidualization in mice and failure of antagonists of PAF to inhibit implantation.
The possible role of platelet-activating factor (PAF) in the uterine responses associated with implantation was investigated. Attempts to trigger a decidual cell response in the uteri of hormonally sensitized, ovariectomized mice by instilling PAF-acether (1-1000 ng) intraluminally were unsuccessful. The effect of PAF antagonists on implantation was investigated in females ovariectomized on Day 3 of pregnancy and treated with progesterone. Implantation was induced in these females by injection of 10 ng oestradiol-17 beta on Day 8. Hourly intraperitoneal injections of three PAF antagonists (WEB 2086, CV 3988 and BN 52021 at doses of 1.2-1.4 mg/kg) given over a 24-h period starting 1 h before the injection of oestradiol-17 beta had no significant effect on the occurrence of implantation sites. Intraluminal injection of WEB 2086 (15 micrograms) or BN 52021 (5 micrograms) either 3 h before or 6 h after the nidatory oestradiol also had no significant inhibitory effect on implantation. SRI 63-441 given once daily over the first 4 days of pregnancy at a dose of 40 micrograms/30 g body weight had no inhibitory effect on the establishment of pregnancy. These results are not consistent with a critical role for PAF in implantation in mice.
Milligan
S R
SR
Department of Physiology, King's College, London, UK.
Finn
C A
CA
D000818:Animals; D001381:Azepines / Q000494:pharmacology; D003656:Decidua / Q000187:drug effects*; D004224:Diterpenes; D010064:Embryo Implantation / Q000187:drug effects*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D046934:Ginkgolides; D007783:Lactones / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D010052:Ovariectomy; D010742:Phospholipid Ethers / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology; D014227:Triazines / Q000494:pharmacology; D014230:Triazoles
D001381:Azepines; D004224:Diterpenes; D046934:Ginkgolides; D007783:Lactones; D010742:Phospholipid Ethers; D010972:Platelet Activating Factor; D014227:Triazines; D014230:Triazoles; C052518:WEB 2086; D011374:Progesterone; D004958:Estradiol; C037913:CV 3988; C045856:ginkgolide B