Guanine tracts enhance sequence directed DNA bends.
Synthetic DNA fragments were constructed to determine the effect of G tracts, in conjunction with periodically spaced A tracts, on DNA bends. Relative length measurements showed that the G tracts spaced at the half helical turn enhanced the DNA bend. When the G tract was interrupted with a thymine or shortened to one or two guanines, the relative lengths decreased. If the G tract was replaced with either an A tract or a T tract, the bend was cancelled. Replacement with a C tract decreased the relative length to that of a thymine interruption suggesting that bend enhancement due to G tracts requires A tracts on the same strand.
Milton
D L
DL
Howard Hughes Medical Institute Laboratory, Department of Human Genetics, University of Utah, Salt Lake City 84132.
Casper
M L
ML
Wills
N M
NM
Gesteland
R F
RF
D001483:Base Sequence; D004247:DNA; D006147:Guanine; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis
D009838:Oligodeoxyribonucleotides; D006147:Guanine; D004247:DNA