Monoclonal antibodies against human synovial phospholipase A2.
Four monoclonal antibodies (HP-1, HP-2, HP-3 and HP-4) with differing reactivities were raised against human synovial fluid phospholipase A2. None of them bound to exocrine phospholipases A2, such as those from pancreas or snake venom. However, antibodies HP-1 and HP-3 showed cross-reactivity with rabbit and rat platelet secretory phospholipases A2, which share common enzymatic and structural features with the human synovial enzyme. Antibodies HP-1, HP-2 and HP-3 inhibited the activity of human synovial phospholipase A2. The antibodies were used to develop a rapid immunoaffinity column chromatographic procedure for enzyme purification. In some preparations, the recovery of total activity after immunoaffinity column chromatography was more than 100% suggesting the existence of endogenous inhibitory factors of phospholipase A2 in human synovial fluid.
Takayama
K
K
Department of Health Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Kudo
I
I
Hara
S
S
Murakami
M
M
Matsuta
K
K
Miyamoto
T
T
Inoue
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D002846:Chromatography, Affinity; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D010740:Phospholipases / Q000032:analysis*; D010741:Phospholipases A / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000276:immunology; D054467:Phospholipases A2; D013582:Synovial Fluid / Q000201:enzymology*
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2