Development and validation of an automated enzyme assay for paracetamol (acetaminophen).
A rapid, enzymatic assay for serum or plasma paracetamol has been developed with the potential for adaptation to a wide range of clinical analysers. The method involves the action of an amidase enzyme to produce 4-aminophenol from paracetamol, which in turn reacts with 8-hydroxyquinoline in the presence of manganese ions to form a blue dye. Two stable reagents are used and excellent precision is achieved over the drug concentration range 0-2.5 mmol/l. The method, which is complete within 6 min, has been validated using a Monarch centrifugal analyser and shows no significant interference from endogenous serum compounds, drugs or paracetamol metabolites.
Morris
H C
HC
Department of Clinical Biochemistry, London Hospital Medical College, UK.
Overton
P D
PD
Ramsay
J R
JR
Campbell
R S
RS
Hammond
P M
PM
Atkinson
T
T
Price
C P
CP
D000082:Acetaminophen / Q000097:blood*; D000581:Amidohydrolases; D001322:Autoanalysis; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D015203:Reproducibility of Results
D007202:Indicators and Reagents; D000082:Acetaminophen; D000581:Amidohydrolases; C019732:aryl acylamidase