Induction and immunohistochemical localization of cytochrome P-450 PCN by non-steroidal compound, in rat liver microsomes.
Induction of cytochrome P-450 PCN (P-450 PCN) by non-steroidal compound, M79193 (cyclohexane spiro-2,6-chloro-1'-(3-dimethyl-aminopropyl) spiro(chroman-4,4'-imidazolidine)-2',5'-dione], was investigated in both sexes of rats. The immunohistochemical localization of P-450 PCN was also studied in liver lobules of untreated and M79193-treated male rats. Immunoblot analysis of rat liver microsomes with anti-P-450 PCN indicated that the amount of P-450 PCN increased 5- to 7-fold by the treatment with M79193, but no increase was observed in P-450 PB-1 (P450IIB1) or P-450 MC-1 (P-450IA1). P-450 PCN was uniformly distributed in liver lobule of untreated rats, and was significantly increased by the treatment with M79193 in both the periportal and pericentral regions of the lobules.
Sagami
F
F
Tsukuba Research Laboratory, Eisai Co. Ltd., Japan.
Tsukidate
K
K
Fukuda
T
T
Nakanowatari
J
J
Horie
T
T
Igarashi
T
T
Kitada
M
M
Kanakubo
Y
Y
D000818:Animals; D001578:Benzopyrans / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D007093:Imidazoles / Q000494:pharmacology*; D048289:Imidazolidines; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000096:biosynthesis*; D008099:Liver / Q000201:enzymology; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D012737:Sex Factors
D001578:Benzopyrans; D007093:Imidazoles; D048289:Imidazolidines; D007527:Isoenzymes; C063623:M 79193; D003577:Cytochrome P-450 Enzyme System