Anti-inflammatory activity of bee venom peptide 401 (mast cell degranulating peptide) and compound 48/80 results from mast cell degranulation in vivo.
1. The relationship between the anti-inflammatory activity of the bee venom peptide 401 in the carrageenin-induced oedema of the rat hind paw and its mast cell degranulating activity has been reinvestigated. 2. Mast cell degranulation caused by compound 48/80 (10 mg kg-1) or by allergen challenge in rats sensitized to Nippostrongylus brasiliensis also suppressed rat hind paw oedema in the same test. 3. The anti-inflammatory activities of peptide 401 and compound 48/80 were partially suppressed by pretreatment of rats with mepyramine and methysergide, at doses (2.5 mg kg-1) that completely suppressed skin reactions to these mast cell-derived amines. Pretreatment of rats with compound 48/80 also suppressed the apparent anti-inflammatory actions of peptide 401 and of compound 48/80. 4. Injection of peptide 401 together with carrageenin increased the inflammatory response in the rat hind paw. 5. The anti-inflammatory activity of peptide 401 and of compound 48/80 in the carrageenin-induced swelling of the rat hind paw arises from mast cell degranulation in vivo.
Banks
B E
BE
Department of Physiology, University College London.
Dempsey
C E
CE
Vernon
C A
CA
Warner
J A
JA
Yamey
J
J
D000485:Allergens / Q000494:pharmacology; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal; D001514:Bee Venoms / Q000494:pharmacology*; D002351:Carrageenan; D002927:Cimetidine / Q000494:pharmacology; D003594:Cytoplasmic Granules / Q000187:drug effects; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D066298:In Vitro Techniques; D007509:Irritants; D008297:Male; D008407:Mast Cells / Q000187:drug effects*; D008784:Methysergide / Q000494:pharmacology; D009559:Nippostrongylus / Q000276:immunology; D010455:Peptides / Q000494:pharmacology*; D011738:Pyrilamine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology*
D000485:Allergens; D000894:Anti-Inflammatory Agents, Non-Steroidal; D001514:Bee Venoms; D007509:Irritants; D010455:Peptides; C008331:mast cell degranulating peptide; D003189:p-Methoxy-N-methylphenethylamine; D002927:Cimetidine; D002351:Carrageenan; D011738:Pyrilamine; D008784:Methysergide