title: Stimulation of myeloid colony growth from peripheral blood by medium with an initially low osmolality.
abstract: The clonal growth of myeloid colonies from peripheral blood was maximal when cultures were established with an initial osmolality of 220 mosmol/kg which increased during incubation as a result of partial drying. When osmolality was stabilized by secondary humidification, the optimum osmolality was 270 mosmol/kg, but growth was always two- to fivefold less than similar cultures established at low osmolality and incubated on an open shelf. Cultures established at 270 mosmol/kg or above were statistically similar whether or not drying was eliminated. Maximum colonies were apparent after 14 days incubation under both conditions; addition of conditioned medium did not alter the pattern of growth. The greater sensitivity of cultures established at 220 mosmol/kg is advantageous when assaying circulating progenitors in pathological conditions where a low number of granulocyte/macrophage colony-forming units is common.
authors.affiliation: Department of Haematology, University of Wales College of Medicine, Heath Park, Cardiff, United Kingdom.
authors.forename: G B
authors.initials: GB
authors.lastname: Tennant
mesh_terms: D003114:Colony-Forming Units Assay / Q000379:methods*; D003470:Culture Media / Q000494:pharmacology*; D046508:Culture Techniques / Q000379:methods; D006410:Hematopoiesis / Q000187:drug effects*; D006801:Humans; D006813:Humidity; D007038:Hypotonic Solutions / Q000494:pharmacology*; D007185:Incubators; D009994:Osmolar Concentration; D013268:Stimulation, Chemical
chemical_list: D003470:Culture Media; D007038:Hypotonic Solutions