Characterization of muscarinic acetylcholine receptors in human labial salivary glands.
Muscarinic acetylcholine receptors were studied in human labial salivary glands with (-)-[3H]MQNB. The radioligand bound with a Kd value of 150 pM. The density of muscarinic receptors was 220 fmol/mg protein. Most muscarinic antagonists bound to a homogeneous population of muscarinic receptors in this tissue. However, the inhibition curves of pirenzepine had a Hill slope of 0.57 and could be analyzed by a two site model. These results suggest that the muscarinic receptors in human labial salivary glands are a mixture of M1 and M3 types.
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Department of Pharmacology, University of Arizona Health Sciences Center, Tucson 85724.
Roeske
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Izutsu
K T
KT
Yamamura
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HI
D000328:Adult; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008954:Models, Biological; D010276:Parasympatholytics / Q000494:pharmacology; D011813:Quinuclidinyl Benzilate; D011976:Receptors, Muscarinic / Q000187:drug effects*; D012469:Salivary Glands / Q000187:drug effects / Q000378:metabolism*
D010276:Parasympatholytics; D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate