title: Smooth muscle myosin light chain kinase: rapid purification by anion exchange high-performance liquid chromatography.
abstract: A rapid procedure for the purification of myosin light chain kinase present in chicken gizzard smooth muscle using anion exchange high-performance liquid chromatography is described. The procedure allows preparation of microgram amounts of the protein directly from the extract of gizzard myofibrils and then is suitable for the study of myosin light chain kinase in small muscles. The protein was judged to be greater than 95% pure by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The enzyme retains its activity since it catalyzes the calcium-calmodulin-dependent phosphorylation of the 20,000-Da myosin light chain.
authors.affiliation: National Research Council Unit for Muscle Biology and Physiopathology, University of Padova, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Dalla Libera
authors.forename: P
authors.initials: P
authors.lastname: Cavallini
authors.forename: M
authors.initials: M
authors.lastname: Fasolo
authors.forename: P
authors.initials: P
authors.lastname: Cavanni
authors.forename: E
authors.initials: E
authors.lastname: Ratti
authors.forename: G
authors.initials: G
authors.lastname: Gaviraghi
mesh_terms: D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D005895:Gizzard, Avian / Q000201:enzymology; D007700:Kinetics; D008970:Molecular Weight; D009130:Muscle, Smooth / Q000201:enzymology*; D009219:Myosin-Light-Chain Kinase / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D009219:Myosin-Light-Chain Kinase