Suppressing effect of antimutagenic flavorings on chromosome aberrations induced by UV-light or X-rays in cultured Chinese hamster cells.
Chromosome aberrations induced by UV-light or X-rays were suppressed by the post-treatment with antimutagenic flavorings, such as anisaldehyde, cinnamaldehyde, coumarin, and vanillin. UV- or X-ray-irradiated surviving cells increased in the presence of each flavoring. X-ray-induced breakage-type and exchange-type chromosome aberrations were suppressed by the vanillin treatment in the G1 phase of the cell cycle and a greater decrease in the number of X-ray-induced chromosome aberrations during G1 holding was observed in the presence of vanillin. Furthermore, a greater decrease in the number of X-ray-induced DNA single-strand breaks was observed in the presence of vanillin. Treatment with vanillin in the G2 phase suppressed UV- and X-ray-induced breakage-type but not exchange-type chromosome aberrations. The suppression of breakage-type aberrations was assumed to be due to a modification of the capability of the post-replicational repair of DNA double-strand breaks. These G1- and G2-dependent anticlastogenic effects were not observed in the presence of 2',3'-dideoxythymidine, an inhibitor of DNA polymerase beta. Based on these results, the anticlastogenic effect of vanillin was considered to be due to the promotion of the DNA rejoining process in which DNA polymerase beta acts.
Sasaki
Y F
YF
Institute of Environmental Toxicology, Tokyo, Japan.
Imanishi
H
H
Watanabe
M
M
Ohta
T
T
Shirasu
Y
Y
D000818:Animals; D001547:Benzaldehydes / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D015224:Dideoxynucleosides / Q000494:pharmacology; D005421:Flavoring Agents / Q000494:pharmacology*; D007399:Interphase; D011837:Radiation-Protective Agents; D014466:Ultraviolet Rays; D014965:X-Rays
D001547:Benzaldehydes; D015224:Dideoxynucleosides; D005421:Flavoring Agents; D011837:Radiation-Protective Agents; C019336:2',3'-dideoxythymidine; C100058:vanillin