Localization of the human A1S9 gene complementing the ts A1S9 mouse L-cell defect in DNA replication and cell cycle progression to Xp11.2----p11.4.
The temperature-sensitive ts A1S9 mouse L-cell mutant is defective in an X-linked gene essential for progression of cells through the S phase of the cell division cycle. A single copy fragment derived from the complementing human A1S9 gene was used as a probe to localize the gene on the X chromosome. Southern blot analysis of human x rodent hybrids and in situ hybridization to human metaphase chromosomes allowed the regional assignment of the human A1S9 gene to Xp11.2----p11.4.
Zacksenhaus
E
E
Department of Microbiology, Faculty of Medicine, University of Toronto, Ontario, Canada.
Sheinin
R
R
Wang
H S
HS
D000818:Animals; D001345:Autoradiography; D015139:Blotting, Southern; D002453:Cell Cycle / Q000235:genetics*; D002874:Chromosome Mapping; D004261:DNA Replication / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D007739:L Cells; D009693:Nucleic Acid Hybridization; D014960:X Chromosome