title: Crystallization and preliminary X-ray characterization of a soybean seed lipoxygenase.
abstract: An isoenzyme of soybean (Glycine max L. Merrill cv. Provar) lipoxygenase (EC 1.13.11.12) has been crystallized using the vapor diffusion method. Crystals were grown from solutions of the protein (7 mg/ml) using 10 to 20% (w/v) polyethylene glycol 8000 in citrate/phosphate buffer (pH 5.7) containing 0.5% (w/v) n-octyl-beta-D-glucopyranoside. The crystals reached maximum dimensions of 0.3 mm x 0.2 mm x greater than 2 mm. The enzyme crystallized in space group C222(1) with unit cell dimensions a = 246 A, b = 193 A and c = 75 A. A calculated Vm value of 2.35 A3/dalton was obtained assuming two molecules per asymmetric unit. The density of the crystals was found to be 1.16 g/ml, which confirmed the presence of two molecules per asymmetric unit and indicated a solvent content of 47.5%.
authors.affiliation: Physical Sciences Center, Monsanto Company, St Louis, MO 63198.
authors.forename: W C
authors.initials: WC
authors.lastname: Stallings
authors.forename: B A
authors.initials: BA
authors.lastname: Kroa
authors.forename: R T
authors.initials: RT
authors.lastname: Carroll
authors.forename: A L
authors.initials: AL
authors.lastname: Metzger
authors.forename: M O
authors.initials: MO
authors.lastname: Funk
mesh_terms: D050557:Catechol 2,3-Dioxygenase; D003460:Crystallization; D049308:Dioxygenases; D007527:Isoenzymes / Q000302:isolation & purification*; D010105:Oxygenases / Q000302:isolation & purification*; D011487:Protein Conformation; D012639:Seeds / Q000201:enzymology; D013025:Glycine max / Q000201:enzymology*; D014961:X-Ray Diffraction
chemical_list: D007527:Isoenzymes; D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase