Ascorbic acid phosphate stimulates type IV collagen synthesis and accelerates adipose conversion of 3T3-L1 cells.
We showed that the synthesis and secretion of type IV collagen, entactin, and laminin were enhanced when adipose conversion of 3T3-L1 cells at confluence was stimulated by hormones (Y. Aratani and Y. Kitagawa (1988) J. Biol. Chem. 263, 16163-16169). Ascorbic acid phosphate (Asc-P) stimulated the synthesis and secretion of type IV collagen and other collagens from both 3T3-L1 preadipocytes and adipocytes. The synthesis and secretion of laminin and entactin were not affected by Asc-P. The continuous addition of Asc-P stimulated cell growth and increased cell density at confluence 1.3-fold. Concomitantly, Asc-P remarkably accelerated the emergence of lipoprotein lipase, glycerophosphate dehydrogenase, and Oil Red O-stainable lipid droplets. These findings suggest an important role for type IV collagen in adipocyte differentiation.
Ono
M
M
School of Agriculture, Nagoya University, Japan.
Aratani
Y
Y
Kitagawa
I
I
Kitagawa
Y
Y
D000273:Adipose Tissue / Q000166:cytology* / Q000187:drug effects; D000818:Animals; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis* / Q000302:isolation & purification; D005353:Fibronectins / Q000096:biosynthesis; D007700:Kinetics; D007797:Laminin / Q000096:biosynthesis; D008715:Methionine / Q000378:metabolism; D051379:Mice
D005353:Fibronectins; D007797:Laminin; C011669:ascorbate-2-phosphate; D003094:Collagen; D008715:Methionine; D001205:Ascorbic Acid