Synthesis of a 60 kD nuclear DNA binding protein induced by cytosine arabinoside in the HL 60 leukemic cell line.
Cytosine arabinoside (ara-C) is being employed at low dosage as differentiative rather than a cytotoxic agent in the therapy of leukemias. We have analyzed nuclear proteins from HL 60 leukemic cells treated with ara-C and have observed increased expression of a 60 kD protein in a dose-dependent fashion. This protein is actively synthesized, as assessed by labeled methionine incorporation. Using DNA cellulose affinity chromatography we could also demonstrate DNA binding properties of the 60 kD protein.
Ravazzolo
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R
Dept. of Biology, I.S.M.I., University of Genova, Italy.
Bianchi-Scarră
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G
Capra
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Fiorentini
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P
Garrĕ
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C
D002460:Cell Line; D002846:Chromatography, Affinity; D003561:Cytarabine / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000032:analysis / Q000097:blood*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007942:Leukemia, Experimental / Q000378:metabolism / Q000473:pathology; D007951:Leukemia, Myeloid / Q000378:metabolism / Q000473:pathology*; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight
D004268:DNA-Binding Proteins; D003561:Cytarabine; D008715:Methionine