Diminished immunogenicity of a recombination-deficient derivative of Vibrio cholerae vaccine strain CVD103.
To address potential concerns over the release of genetically engineered live bacterial vaccines, we constructed a recombination-deficient derivative of the Vibrio cholerae O1 vaccine strain CVD103 (CVD103RM). Oral immunization of adult volunteers with CVD103RM showed that the recA mutation significantly diminished colonization ability and immunogenicity of the vaccine strain.
Ketley
J M
JM
Department of Medicine, University of Maryland, Baltimore 21201.
Kaper
J B
JB
Herrington
D A
DA
Losonsky
G
G
Levine
M M
MM
D000907:Antibodies, Bacterial / Q000096:biosynthesis; D022121:Cholera Vaccines / Q000276:immunology*; D003001:Cloning, Molecular; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D009154:Mutation; D011938:Rec A Recombinases / Q000235:genetics; D011995:Recombination, Genetic; D014611:Vaccination; D014734:Vibrio cholerae / Q000235:genetics / Q000276:immunology*
D000907:Antibodies, Bacterial; D022121:Cholera Vaccines; D011938:Rec A Recombinases