Characterization of two monoclonal antibodies which recognize different subpopulations of chicken T lymphocytes.
Distribution among peripheral T lymphocyte subpopulations and biochemical properties of the chicken lymphocyte surface antigens defined by monoclonal antibodies (mAbs) Lc-4 and Lc-6 were examined. Two-color immunofluorescence analysis revealed that Lc-4 and Lc-6 antigens were expressed on mutually exclusive subpopulations of peripheral T lymphocytes but not on B lymphocytes. Lc-4 mAb precipitated a polypeptide with apparent molecular mass of 35 and 65 kilodalton under reducing and non-reducing conditions, respectively. These results indicated that the antigen recognized with Lc-4 was closely similar in tissue distribution and biochemical property to mammalian CD8 antigen.
Kondo
T
T
Department of Epizootiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Hattori
M
M
Kodama
H
H
Onuma
M
M
Mikami
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D002478:Cells, Cultured; D002645:Chickens / Q000276:immunology*; D003370:Coturnix; D005434:Flow Cytometry; D011233:Precipitin Tests; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface