title: Capillary electrophoresis with indirect amperometric detection.
abstract: The use of indirect amperometric detection with capillary electrophoresis is demonstrated. The system consists of a porous glass coupler which allows amperometric detection at a carbon fiber electrode placed in the end of the capillary. 3,4-Dihydroxybenzylamine is added to the buffer system as a continuously eluting electrophore. Indirect amperometric detection in 9-mumol I.D. capillaries provides detection limits as low as 380 attomole for the amino acid arginine. Finally, both direct and indirect amperometric detection can be accomplished simultaneously.
authors.affiliation: Department of Chemistry, Penn State University, University Park 16802.
authors.forename: T M
authors.initials: TM
authors.lastname: Olefirowicz
authors.forename: A G
authors.initials: AG
authors.lastname: Ewing
mesh_terms: D000596:Amino Acids / Q000032:analysis; D004553:Electric Conductivity; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D010455:Peptides / Q000032:analysis
chemical_list: D000596:Amino Acids; D010455:Peptides