Adriamycin cellular transport: methodological aspects.
A rapid and simple method for determination of cellular uptake of adriamycin is described. The method is based on the principle that active uptake is proportional to alterations of drug distribution, measured as a fraction of time, between suspending medium and cells, the volume of each having been accurately determined. Cellular drug uptake can be calculated by the use of a simple distribution formula. This method represents a compromise between indirect measurement of the loss of drug from suspending medium and direct measurement of drug uptake following cell separation, washing, and lysis. This method should be applicable to the measurement of cellular uptake of a wide range of drugs.
Havlik
I
I
Department of Pharmacology, University of the Witwatersrand, Medical School, Johannesburg, South Africa.
Dansey
R D
RD
Keeping
J C
JC
Golombick
T
T
Bezwoda
W R
WR
D001692:Biological Transport; D002460:Cell Line; D004317:Doxorubicin / Q000032:analysis / Q000378:metabolism / Q000493:pharmacokinetics*; D006801:Humans; D013997:Time Factors
D004317:Doxorubicin