H-2 class I gene analysis in 3-methylcholanthrene-induced murine fibrosarcomas.
The objective of our study was to determine whether and how frequently MHC class I gene alterations occur in fibrosarcomas induced in BALB/c mice by the carcinogen 3-methylcholanthrene (3-MCA). Southern blot was performed to analyze the genomic organization of class I genes in a panel of twenty tumors of different immunogenic strength used at early in vivo passages. Our results rule out the presence of gross rearrangements or deletions in the H-2 class I genes of the tumors tested and indicate that the frequency of detectable class I alterations giving rise to restriction fragments length polymorphism (RFLP) should be very low.
Castelli
C
C
Division of Experimental Oncology D, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milan, Italy.
Mazzucchelli
F
F
Sensi
M
M
Carbone
G
G
Parmiani
G
G
D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics*; D015139:Blotting, Southern; D015342:DNA Probes; D005260:Female; D005354:Fibrosarcoma / Q000139:chemically induced / Q000235:genetics* / Q000276:immunology; D015972:Gene Expression Regulation, Neoplastic; D005805:Genes, MHC Class I; D006183:H-2 Antigens / Q000235:genetics*; D008748:Methylcholanthrene; D051379:Mice; D008807:Mice, Inbred BALB C / Q000235:genetics; D005810:Multigene Family
D000951:Antigens, Neoplasm; D015342:DNA Probes; D006183:H-2 Antigens; D008748:Methylcholanthrene