Bovine monoclonal antibodies specific for bovine herpesvirus-1 glycoprotein gIII.
Spleen cells from a calf immunized with bovine herpesvirus-1 (BHV-1) were fused with the nonsecreting murine cell line SP2/0. Several bovine-murine hybridomas secreting bovine immunoglobulins were stabilized. Of these, 9 hybridomas secreted bovine monoclonal antibodies that specifically bound to BHV-1 in a radioimmunoassay. Two of these monoclonal antibodies reacted specifically with BHV-1 in an indirect fluorescent antibody test and immunoprecipitated a BHV-1 glycoprotein with molecular mass of 97 kilodaltons.
Srikumaran
S
S
Department of Veterinary Science, University of Nebraska, Lincoln 68583-0905.
Onisk
D V
DV
Zamb
T J
TJ
Osorio
F A
FA
Rice
D N
DN
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000378:metabolism; D000918:Antibody Specificity; D002417:Cattle / Q000382:microbiology*; D002460:Cell Line; D006825:Hybridomas / Q000276:immunology; D008297:Male; D051379:Mice; D014764:Viral Proteins / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins