Dietary-induced pre-translational control of rat fatty acid synthase.
We have examined the effects of starvation, normal lab chow and low-fat carbohydrate-rich diet on rat fatty acid synthase (FAS, EC 2.3.1.85). Under each of the dietary conditions the amount of FAS mRNA is different, the most being produced after a low-fat carbohydrate-rich diet. There is also an increase in the amount of FAS protein under the same conditions. To complete the picture we determined the incorporation of [14C]acetate into palmitate as a measure of enzyme activity. Data for cardiac and renal tissue also reflect a dietary influence on FAS. Therefore FAS mRNA, FAS protein and FAS activity are all responsive to dietary-induced signals, and our results suggest a pre-translational regulation.
Laux
T
T
Institut für Mikrobiologie und Biochemie, Universität Erlangen-Nürnberg, Federal Republic of Germany.
Schweizer
M
M
D000818:Animals; D004032:Diet; D064429:Fatty Acid Synthases / Q000096:biosynthesis / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic; D007668:Kidney / Q000201:enzymology / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000201:enzymology / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D012333:RNA, Messenger; D064429:Fatty Acid Synthases