Developmental changes in the antigenicity and sugar-chain heterogeneity of rabbit alkaline phosphatases.
1. Rabbit alkaline phosphatases (APs) clearly fused with the anti-human AP antibodies. In particular, fetal liver and kidney APs reacted slightly less with the anti-intestinal AP antibody as did adult enzymes, suggesting that intestinal AP-like isozyme is expressed at earlier stages of gestation in rabbit liver and kidney. 2. Immunohistochemical data indicated that intestinal AP-like isozyme in the kidney was mainly localized in the distal convoluted tubules and slightly in the proximal straight tubules, whereas liver/bone/kidney AP-like enzyme was found more in the glomeruli and interstitial capillary walls as a major component. 3. The sugar-chain heterogeneity of adult and fetal rabbit APs displayed organ-specificity as did of rat and human APs. Moreover, in fetal development, the expression of high-mannose type or hybrid type sugar chains precedes the expression of complex type sugar chains in fetal development.
Arai
Y
Y
Department of Biochemistry, Saitama Medical School, Japan.
Koyama
I
I
Hirota
N
N
Sakai
T
T
Sakagishi
Y
Y
Komoda
T
T
D000375:Aging / Q000276:immunology / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D000941:Antigens / Q000032:analysis*; D002846:Chromatography, Affinity; D003429:Cross Reactions / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D005314:Embryonic and Fetal Development / Q000502:physiology*; D005260:Female; D007120:Immunochemistry; D007422:Intestines / Q000196:embryology / Q000201:enzymology; D007527:Isoenzymes / Q000378:metabolism; D007668:Kidney / Q000196:embryology / Q000201:enzymology; D008099:Liver / Q000196:embryology / Q000201:enzymology; D011247:Pregnancy; D011817:Rabbits
D000941:Antigens; D007527:Isoenzymes; D000469:Alkaline Phosphatase