title: Immunocytochemical determination of ploidy class-dependent bromodeoxyuridine incorporation in rat liver parenchymal cells after partial hepatectomy.
abstract: Immunocytochemistry of bromodeoxyuridine (BrdU) incorporated in DNA was performed on cryostat sections of rat liver and on isolated hepatocytes after partial hepatectomy using a two-step labeling technique. The method enabled the detection of S-phase nuclei in both tissue preparations. Quantification of the number of labeled nuclei in sections showed that the number of nuclei in S-phase increased from 0.3% in control liver to about 36% at 24 h after partial hepatectomy. The detection of BrdU in isolated hepatocytes showed the same labeling index of binuclear diploid, mononuclear tetraploid and binuclear tetraploid cells. A special role for mononuclear diploid cells in proliferation did not seem to occur.
authors.affiliation: Laboratory of Cell Biology and Histology, University of Amsterdam, The Netherlands.
authors.forename: W M
authors.initials: WM
authors.lastname: Frederiks
authors.forename: F
authors.initials: F
authors.lastname: Marx
authors.forename: R A
authors.initials: RA
authors.lastname: Chamuleau
authors.forename: C J
authors.initials: CJ
authors.lastname: van Noorden
authors.forename: J
authors.initials: J
authors.lastname: James
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine; D002455:Cell Division; D004247:DNA / Q000096:biosynthesis*; D006498:Hepatectomy; D007150:Immunohistochemistry; D008099:Liver / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D011003:Ploidies; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004247:DNA; D001973:Bromodeoxyuridine