Effect of the multicatalytic proteinase (prosome) on translational activity in rabbit reticulocyte lysates.
In a message-dependent reticulocyte lysate translation system, incorporation of [3H]leucine into acid-insoluble protein is increased following selective removal of the multicatalytic proteinase (MCP) with a monospecific antibody. Re-addition of active proteinase to previously depleted lysates reverses this effect in that the same low levels of translational product are measured as in untreated lysates. Addition of histone-stimulated MCP further depresses the level of protein product. Conversely, lysates supplemented with inactivated MCP retain the higher level of translational activity which is measured after precipitation of the enzyme with antibody. In these lysates, the effect of the antibody on translational activity is inversely correlated with that on hydrolytic activity towards [14C]methylcasein or N-succinyl-Leu-Leu-Val-Tyr-4-methyl-7-coumarylamide, two substrates of the MCP. These results showing that the MCP is capable of modulating translational activity in vitro, suggest an important role of this molecule in the in vivo translational process.
Kuehn
L
L
Diabetes Forschungsinstitut, Düsseldorf, FRG.
Dahlmann
B
B
Kopp
F
F
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000494:pharmacology; D003546:Cysteine Endopeptidases / Q000276:immunology / Q000494:pharmacology / Q000502:physiology*; D005914:Globins / Q000235:genetics; D006657:Histones / Q000494:pharmacology; D007074:Immunoglobulin G / Q000276:immunology / Q000494:pharmacology; D008099:Liver / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics; D009097:Multienzyme Complexes / Q000276:immunology / Q000494:pharmacology / Q000502:physiology*; D011132:Polyribosomes / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex; D014176:Protein Biosynthesis / Q000187:drug effects / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D012156:Reticulocytes / Q000378:metabolism*; D014027:Tobacco Mosaic Virus / Q000235:genetics
D000906:Antibodies; D006657:Histones; D007074:Immunoglobulin G; D009097:Multienzyme Complexes; D012333:RNA, Messenger; D012367:RNA, Viral; D005914:Globins; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex