Rapid labeling of lipoproteins in plasma with radioactive cholesterol. Application for measurement of plasma cholesterol esterification.
In order to efficiently and rapidly label lipoproteins in plasma with [3H]cholesterol, micelles consisting of lysophosphatidylcholine (lysoPC) and [3H]cholesterol (molar ratio, 50:1) were prepared. When trace amounts of these micelles were injected into plasma, [3H]cholesterol rapidly equilibrated among the plasma lipoproteins, as compared to [3H]cholesterol from an albumin-stabilized emulsion. The distributions of both [3H]cholesterol and unlabeled free cholesterol in plasma lipoproteins were similar in labeled plasma samples. This method of labeling can be used for the measurement of cholesterol esterification, or lecithin:cholesterol acyltransferase activity, in small amounts (20-40 microliters) of plasma samples.
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Burnsides Research Laboratory, Department of Food Science, University of Illinois, Urbana 61801.
Nishida
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D002784:Cholesterol / Q000097:blood*; D002788:Cholesterol Esters / Q000097:blood*; D004951:Esterification; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D008244:Lysophosphatidylcholines; D008823:Micelles; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000097:blood; D014316:Tritium
D002788:Cholesterol Esters; D008074:Lipoproteins; D008244:Lysophosphatidylcholines; D008823:Micelles; D014316:Tritium; D002784:Cholesterol; D007862:Phosphatidylcholine-Sterol O-Acyltransferase