title: A new inhibitor of protein kinase C, RK-286C (4'-demethylamino-4'-hydroxystaurosporine). II. Isolation, physico-chemical properties and structure.
abstract: RK-286C, a new inhibitor of protein kinase C, has been found by the bleb-forming assay using K562 cells. It was produced by Streptomyces sp. RK-286 and purified by solvent extraction, silica gel chromatography and preparative HPLC. Spectrometric analysis revealed that the structure is 4'-demethylamino-4'-hydroxystaurosporine.
authors.affiliation: Antibiotics Laboratory, RIKEN, Institute of Physical and Chemical Research, Saitama, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
authors.forename: H
authors.initials: H
authors.lastname: Osada
authors.forename: M
authors.initials: M
authors.lastname: Uramoto
authors.forename: K
authors.initials: K
authors.lastname: Isono
mesh_terms: D000470:Alkaloids / Q000032:analysis / Q000302:isolation & purification*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D013053:Spectrophotometry; D019311:Staurosporine / Q000031:analogs & derivatives; D013302:Streptomyces / Q000032:analysis
chemical_list: D000470:Alkaloids; D003470:Culture Media; C063438:4'-demethylamino-4'-hydroxystaurosporine; D011493:Protein Kinase C; D019311:Staurosporine