Thymidine kinase in human leukemia--expression of three isoenzyme variants in six patients with chronic myelocytic leukemia.
In six patients with untreated, chronic myelocytic leukemia (CML), the dominating thymidine kinase (TK) activity was compared with the fetal form, TK 1, from mitogen stimulated and the adult form TK 2 from unstimulated normal human lymphocytes, and with TK-1-onc, TK-3-onc and TK-4-onc. This was done in human acute, myelocytic and monocytic leukemias, using the combined thymidine/dTTP enzyme kinetics for isoenzyme characterization. TK-1-onc was found in one, TK-2-onc in two and TK-3-onc in three CML patients. The suffix -onc indicates the difference in ATP kinetics and molecular weights between the normal and the leukemic thymidine kinases. A possible relation between the isoenzyme forms and the types of leukemias is discussed.
Munch-Petersen
B
B
Roskilde University, Institute of Life Sciences and Chemistry, Denmark.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007700:Kinetics; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood / Q000201:enzymology*; D007963:Leukocytes, Mononuclear / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D013937:Thymidine Kinase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013942:Thymine Nucleotides / Q000494:pharmacology
D007527:Isoenzymes; D013942:Thymine Nucleotides; D000255:Adenosine Triphosphate; D013937:Thymidine Kinase; C024157:thymidine 5'-triphosphate