Constructing deletions with defined endpoints in Drosophila.
Chromosomes bearing small deletions are valuable tools in Drosophila genetics. We have investigated a method for efficiently constructing precise chromosomal deficiencies. Two P transposable elements were positioned within a progenitor strain at the sites of the desired deletion endpoints. Deletions spanning the two transposons were recovered at high frequency when P element transposase was expressed in these flies, but only if the flanking P elements were in a cis rather than a trans configuration. Appropriate progenitor strains can now be constructed to delete virtually any chromosomal region by utilizing an extensive collection of lines containing single P element insertions throughout the Drosophila genome.
Cooley
L
L
Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21210.
Thompson
D
D
Spradling
A C
AC
D000818:Animals; D015139:Blotting, Southern; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002875:Chromosomes / Q000648:ultrastructure; D004247:DNA / Q000235:genetics; D004330:Drosophila / Q000235:genetics*; D005838:Genotype; D015183:Restriction Mapping
D004247:DNA