title: Thymidine kinase in human leukemia--expression of three isoenzyme variants in six patients with chronic myelocytic leukemia.
abstract: In six patients with untreated, chronic myelocytic leukemia (CML), the dominating thymidine kinase (TK) activity was compared with the fetal form, TK 1, from mitogen stimulated and the adult form TK 2 from unstimulated normal human lymphocytes, and with TK-1-onc, TK-3-onc and TK-4-onc. This was done in human acute, myelocytic and monocytic leukemias, using the combined thymidine/dTTP enzyme kinetics for isoenzyme characterization. TK-1-onc was found in one, TK-2-onc in two and TK-3-onc in three CML patients. The suffix -onc indicates the difference in ATP kinetics and molecular weights between the normal and the leukemic thymidine kinases. A possible relation between the isoenzyme forms and the types of leukemias is discussed.
authors.affiliation: Roskilde University, Institute of Life Sciences and Chemistry, Denmark.
authors.forename: B
authors.initials: B
authors.lastname: Munch-Petersen
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007700:Kinetics; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood / Q000201:enzymology*; D007963:Leukocytes, Mononuclear / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D013937:Thymidine Kinase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013942:Thymine Nucleotides / Q000494:pharmacology
chemical_list: D007527:Isoenzymes; D013942:Thymine Nucleotides; D000255:Adenosine Triphosphate; D013937:Thymidine Kinase; C024157:thymidine 5'-triphosphate