Characterization of a monoclonal antibody specific for prostatic secretory protein of 94 amino acids (PSP94) and development of a two-site binding enzyme immunoassay for PSP94.
Purified prostatic secretory protein of 94 amino acids (PSP94) was used to generate polyclonal rabbit anti-PSP94 IgG and a murine monoclonal antibody 6B6 (mAB6B6). Both antibodies were highly specific for PSP94 by immunoblotting. Immunohistochemical studies demonstrated the specificity of mAB6B6 for human prostatic epithelial tissue. A two-site binding enzyme immunoassay for the detection of PSP94 was developed using a combination of the antibodies. The sandwich-ELISA yielded satisfactory results when mAB6B6 complexed to peroxidase conjugated goat anti-mouse-IgG (Fc) was incubated simultaneously with the sample. The assay has a dynamic range of 2-30 micrograms/l. This immunoassay was employed to measure PSP94 in male human sera (8 +/- 4 micrograms/l), female human sera (5.7 +/- 3.4 micrograms/l), follicular fluid (3.9 +/- 2.9 micrograms/l) and human seminal plasma (1.02 +/- 0.8 g/l).
von der Kammer
H
H
Max-Planck-Institut für biophysikalische Chemie, Department of Molecular Biology, Göttingen, FRG.
Krauhs
E
E
Aumüller
G
G
Scheit
K H
KH
D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015571:Follicular Fluid / Q000032:analysis*; D006801:Humans; D008297:Male; D010455:Peptides / Q000032:analysis / Q000097:blood* / Q000276:immunology; D029584:Prostatic Secretory Proteins; D012661:Semen / Q000032:analysis*
D000911:Antibodies, Monoclonal; D010455:Peptides; D029584:Prostatic Secretory Proteins; C043076:beta-microseminoprotein