title: A finger protein structurally similar to TFIIIA that binds exclusively to 5S RNA in Xenopus.
abstract: A 5S RNA binding protein (p43) in Xenopus is a major constituent of oocytes and comprises part of a 42S ribonucleoprotein storage particle. We have cloned and sequenced p43 cDNA from X. laevis and X. borealis as well as the cDNA for X. borealis TFIIIA. Like TFIIIA, p43 has nine zinc fingers, seven of which are exactly the same size as their counterparts in TFIIIA. Amino acid homology between the two proteins is restricted mainly to conserved residues characteristic of zinc fingers. In contrast to TFIIIA, which binds specifically to both 5S RNA and 5S RNA genes, p43 binds exclusively to 5S RNA.
authors.affiliation: Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21210.
authors.forename: K E
authors.initials: KE
authors.lastname: Joho
authors.forename: M K
authors.initials: MK
authors.lastname: Darby
authors.forename: E T
authors.initials: ET
authors.lastname: Crawford
authors.forename: D D
authors.initials: DD
authors.lastname: Brown
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000378:metabolism; D005260:Female; D015723:Gene Library; D005796:Genes; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism*; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics; D012335:RNA, Ribosomal / Q000378:metabolism*; D012341:RNA, Ribosomal, 5S / Q000378:metabolism*; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins / Q000096:biosynthesis / Q000302:isolation & purification; D012269:Ribosomal Proteins; D012689:Sequence Homology, Nucleic Acid; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D029867:Xenopus Proteins; D014982:Xenopus laevis
chemical_list: D002352:Carrier Proteins; C489267:LOC397745 protein, Xenopus; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012341:RNA, Ribosomal, 5S; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; D012269:Ribosomal Proteins; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors; D029867:Xenopus Proteins; D004247:DNA