Cloning and expression of the dermonecrotic toxin gene of Pasteurella multocida ssp. multocida in Escherichia coli.
A DNA library of Pasteurella multocida ssp. multocida strain CVI 47459 was constructed in the Lambda GEM-11 vector. Recombinant clones that encoded dermonecrotic toxin (DNT) were identified immunologically with antiserum raised against purified DNT. By comparing the DNA restriction maps of the immunoreactive recombinants, we located the DNT gene. Hybridization studies with 10 strains of P. multocida ssp. multocida suggested that strains that do not produce the DNT do not contain sequences homologous to the DNT gene.
Kamps
A M
AM
Department of Bacteriology, Central Veterinary Institute, Lelystad, The Netherlands.
Kamp
E M
EM
Smits
M A
MA
D001427:Bacterial Toxins / Q000235:genetics*; D003001:Cloning, Molecular; D003885:Dermotoxins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D009693:Nucleic Acid Hybridization; D010325:Pasteurella / Q000235:genetics*; D015183:Restriction Mapping
D001427:Bacterial Toxins; D003885:Dermotoxins; C052804:dermonecrotic toxin, Pasteurella multocida