Properties of alkaline phosphatase of the human dental pulp.
Enzymatic and immunological properties of alkaline phosphatase [ALP; orthophosphoric monoester phosphohydrolase, alkaline optimum, EC 3.1.3.1.] in the human dental pulp were investigated. In inhibition and thermal inactivation studies, dental pulp ALP showed properties of universal-type ALP (kidney/bone/liver type). Dental pulp ALP cross-reacted with polyclonal and monoclonal antibodies against purified swine-kidney ALP, and with monoclonal antibody against ALP of human osteoblast-like cells in the same manner as ALPs of human bone and kidney. The sodium dodecyl sulfate-gel electrophoretic pattern showed a 140,000-Mr native protein band. These data suggest that dental pulp ALP can be classified as a universal-type ALP having antigenic determinants common to ALP of the kidney and bone.
Goseki
M
M
Department of Biochemistry, Tokyo Medical and Dental University, Japan.
Oida
S
S
Nifuji
A
A
Sasaki
S
S
D000469:Alkaline Phosphatase / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D003782:Dental Pulp / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D006801:Humans; D051379:Mice; D011817:Rabbits; D013552:Swine
D000911:Antibodies, Monoclonal; D000469:Alkaline Phosphatase