Automated determination of excitatory amino acid neurotoxicity in cortical culture.
We used a commercially available robotic laboratory workstation to quantitatively study excitotoxic neuronal injury in cell culture. A Beckman Instruments Biomek 1000 was programmed to perform both timed exposures to excitatory amino acid agonists, and kinetic assay of the resultant efflux of lactic dehydrogenase from damaged neurons, using 96-well culture plates. Examination of homocysteate neurotoxicity utilizing this automated method produced results similar to those obtained earlier using manual techniques. The method described here may facilitate the characterization of neurotoxic agonist or antagonist activity.
Klingman
J G
JG
Department of Neurology, Stanford Medical Center, CA 94305.
Hartley
D M
DM
Choi
D W
DW
D000596:Amino Acids / Q000633:toxicity*; D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006710:Homocysteine / Q000031:analogs & derivatives* / Q000633:toxicity; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D051379:Mice; D009498:Neurotoxins / Q000494:pharmacology*
D000596:Amino Acids; D009498:Neurotoxins; D006710:Homocysteine; C007956:homocysteic acid; D007770:L-Lactate Dehydrogenase