Automated semen analysis shows an increase in sperm concentration and motility with time in Makler chambers having excess sample volume.
The sperm concentration and motility within a Makler chamber increased markedly after 3-5 min of settling when one large drop (approximately 24 microliters) of semen was loaded in the chamber. The values remained stable when a 5-microliter volume was loaded and were intermediate with a 10-microliter volume. In order to obtain accurate data, it is necessary to load the Makler chamber with a semen volume of no more than 5 microliters.
Yanagida
K
K
Department of Obstetrics and Gynaecology, Fukushima Medical College, Japan.
Hoshi
K
K
Sato
A
A
Burkman
L
L
D001331:Automation; D006801:Humans; D008297:Male; D012661:Semen / Q000166:cytology* / Q000502:physiology; D013048:Specimen Handling; D013076:Sperm Count / Q000295:instrumentation*; D013081:Sperm Motility; D013997:Time Factors