Dexamethasone decreases membrane fluidity of leukemia cells.
Lipid fluidity in the plasma membrane of leukemia cells was determined by measuring steady-state fluorescence polarization (P) of 1,6-diphenyl-1,3,5-hexatriene. In vitro dexamethasone treatment induced a dose-, time- and temperature-dependent and reversible increase in P values of primary leukemia cells and glucocorticoid-sensitive leukemia cell lines having specific glucocorticoid receptors. Membrane fluidity of glucocorticoid-resistant subclones with impaired specific dexamethasone binding capacity was not influenced by the drug. The results of this study suggest that dexamethasone modulates leukemia cell membrane fluidity via a classical glucocorticoid receptor dependent pathway.
Kiss
C
C
Department of Pediatrics, Medical University of Debrecen, Hungary.
Balázs
M
M
Kéri-Fülöp
I
I
D001752:Blast Crisis / Q000378:metabolism; D002460:Cell Line; D002478:Cells, Cultured; D002648:Child; D003907:Dexamethasone / Q000378:metabolism / Q000494:pharmacology*; D004161:Diphenylhexatriene; D006801:Humans; D007700:Kinetics; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000378:metabolism* / Q000473:pathology; D011965:Receptors, Glucocorticoid / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism*
D011965:Receptors, Glucocorticoid; D004161:Diphenylhexatriene; D003907:Dexamethasone