title: A study in the maintenance phase of ischaemic acute renal failure in the rat.
abstract: The fate of the trapped deformed erythrocytes seen in the early recirculation phase after ischaemia and the generation of long Tamm-Horsfall (TH) cylinders in the renal medulla during the first week after recirculation was studied in rats. In an in-vitro study the effects of different concentrations of TH protein on the permeability to Na+ of a semipermeable membrane were also investigated. The trapping of erythrocytes was found to be a reflow phenomenon, as there was no increase in the capillary area of the medulla in kidneys subjected to ischaemia but with no recirculation. This area increased to a maximum of 34.6 +/- 2.07% 20 min after recirculation and decreased to a normal value of 3.3 +/- 0.74% 1 day after the primary ischaemia. The area occupied by cylinders increased to a maximum of 19.2 +/- 1.4% 2 days after the primary damage and was as large as 16.7 +/- 1.47% after 1 week. It was also shown that the diffusion half-time of Na+ ions across a semipermeable membrane increased from 11.4 +/- 0.45 min to a maximum of 32.2 +/- 2.19 min with a protein concentration of 1 mg ml-1. It was concluded that the trapping of erythrocytes alone could not explain the decrease in renal function 1 week after the primary damage, but that the blockade of the tubules by the long homogeneous TH cylinders could be responsible for this decrease.
authors.affiliation: Department of Physiology and Medical Biophysics, University of Uppsala, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Bayati
mesh_terms: D058186:Acute Kidney Injury / Q000473:pathology / Q000503:physiopathology*; D000818:Animals; D002196:Capillaries / Q000473:pathology; D004912:Erythrocytes / Q000473:pathology; D066298:In Vitro Techniques; D007511:Ischemia / Q000473:pathology / Q000503:physiopathology*; D007668:Kidney / Q000098:blood supply* / Q000473:pathology / Q000503:physiopathology; D009088:Mucoproteins / Q000378:metabolism; D009929:Organ Size; D010539:Permeability; D051381:Rats; D011919:Rats, Inbred Strains; D058949:Uromodulin
chemical_list: D009088:Mucoproteins; C548614:Umod protein, rat; D058949:Uromodulin