title: Identification and mapping of a common proviral integration site Fli-1 in erythroleukemia cells induced by Friend murine leukemia virus.
abstract: Friend murine leukemia virus (F-MuLV) induces erythroleukemia when inoculated into newborn BALB/c or NIH/Swiss mice. We have molecularly cloned F-MuLV host cell DNA junction fragments from an erythroleukemia cell line induced by F-MuLV to identify cellular genes involved in the leukemogenic process. One particular proviral integration site, Fli-1, is rearranged in 75% (9/12) of independently isolated erythroleukemia cell lines derived from either BALB/c or NIH/Swiss mice inoculated at birth with F-MuLV. Other hematopoietic neoplasms induced by F-MuLV, including myeloid (granulocytic) and lymphoid tumors, did not show rearrangements of the Fli-1 locus. Similarly, none of 35 erythroleukemia cell lines induced by the Friend virus complexes (FV-A and FV-P) was rearranged at the Fli-1 locus. In contrast, no rearrangements were detected at the Sfpi-1 locus, a preferred site of integration in either FV-P- or FV-A-induced leukemias. Using recombinant inbred mice, the Fli-1 locus was situated on mouse chromosome 9 close to the cellular protooncogene c-ets-1. DNA and RNA analysis suggests, however, that Fli-1 is different from ets-1. Thus, Fli-1 appears to define a distinct locus specifically involved in the induction of erythroid leukemias by F-MuLV.
authors.affiliation: Division of Molecular and Developmental Biology, Mount Sinai Hospital Research Institute, Toronto, ON, Canada.
authors.forename: Y
authors.initials: Y
authors.lastname: Ben-David
authors.forename: E B
authors.initials: EB
authors.lastname: Giddens
authors.forename: A
authors.initials: A
authors.lastname: Bernstein
mesh_terms: D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D005622:Friend murine leukemia virus / Q000235:genetics* / Q000302:isolation & purification; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics / Q000382:microbiology*; D007942:Leukemia, Experimental / Q000235:genetics / Q000382:microbiology*; D051379:Mice; D008815:Mice, Inbred Strains; D009693:Nucleic Acid Hybridization; D011533:Proviruses / Q000235:genetics* / Q000302:isolation & purification; D015183:Restriction Mapping
chemical_list: D015342:DNA Probes; D004273:DNA, Neoplasm; D004279:DNA, Viral