Cloning and analysis of a 35.3-kilobase DNA region involved in exopolysaccharide production by Xanthomonas campestris pv. campestris.
Cosmid clones able to restore exopolysaccharide production in possibly insertion sequence element-induced surface mutants of Xanthomonas campestris pv. campestris were isolated. By fragment-specific Tn5-lac mutagenesis of one of the cosmids, pXCB1002, a new DNA region which is involved in exopolysaccharide biosynthesis and which is organized into at least 12 complementation groups was identified.
Hötte
B
B
Lehrstuhl für Genetik, Fakultät für Biologie, Universität Bielefeld, Federal Republic of Germany.
Rath-Arnold
I
I
Pühler
A
A
Simon
R
R
D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003360:Cosmids; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D015723:Gene Library; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D010641:Phenotype; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D014974:Xanthomonas / Q000235:genetics*
D004269:DNA, Bacterial; D011135:Polysaccharides, Bacterial