[Radioisotopic assay of total L-homocysteine in plasma and urine: application to serial determinations].
The authors report here a simple radioenzymatic determination of total L-homocysteine in plasma and urine. L-homocysteine-containing disulfides were reduced with dithioerythritol. L-homocysteine was then condensed by S-adenosyl L-homocysteine hydrolase to 14C-adenosine to form S-14C adenosyl L-homocysteine that was separated from 14C-adenosine by paper descendant chromatography. The concentration of the total plasma L-homocysteine of 45 normal subjects was 8.04 +/- 0.26 (mean +/- SEM) mumol/l and total L-homocysteine concentration in urine was 0.59 +/- 0.06 mumol/mmol of creatinine (25 subjects). This method is as sensitive than the other methods described in the literature but more rapid and less expensive.
Chadefaux
B
B
Laboratoire de Biochimie Médicale B, Hôpital Necker Enfants Malades, Paris.
Coude
M
M
Hamet
M
M
Bienvenu
T
T
Fraigne
P
P
Aupetit
J
J
Kamoun
P
P
D000328:Adult; D002250:Carbon Radioisotopes; D002851:Chromatography, High Pressure Liquid; D002854:Chromatography, Paper; D005260:Female; D006710:Homocysteine / Q000097:blood* / Q000652:urine; D006801:Humans; D008297:Male; D008875:Middle Aged
D002250:Carbon Radioisotopes; D006710:Homocysteine
Dosage radioisotopique de la L-homocystéine totale dans le plasma et l'urine: application à des dosages en séries.