The culture of human skin fibroblasts on microcarriers in the presence of serum substitute: Ultroser G.
The effects of the substitution of serum by Ultroser G on human skin fibroblasts cultured on microcarriers were analysed. Cultures could not be established on microcarriers in the presence of Ultroser G. However, microcarrier cultures started in the presence of 10% foetal calf serum, and transferred to 2% Ultroser G after 7 days resulted in high cell densities.
Miech
G
G
Laboratoire de Biochimie Appliquée, Faculté de Pharmacie, Chatenay-Malabry, France.
Myara
I
I
Mangeot
M
M
Gautier
M
M
Lemonnier
A
A
D001802:Blood Substitutes; D002021:Buffers; D002455:Cell Division; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D008863:Microspheres; D009930:Organic Chemicals
D001802:Blood Substitutes; D002021:Buffers; D009930:Organic Chemicals; C044609:Ultroser G