The synthesis of polyamide-oligonucleotide conjugate molecules.
We have developed methods for the synthesis of peptide-oligodeoxyribonucleotide conjugate molecules in particular, and polyamide-oligonucleotide conjugates in general. Synthesis is carried out by a solid-phase procedure and involves the assembly of a polyamide on the solid support, conversion of the terminal amino group to a protected primary aliphatic hydroxy group by reaction with alpha, omega-hydroxycarboxylic acid derivatives, and finally oligonucleotide synthesis using phosphoramidite chemistry. The conjugate molecules can be used as DNA probes, with the polyamide component carrying one or more non-radioactive markers. These conjugates also have the potential to be used as anti-sense inhibitors of gene expression, with the peptide segment acting as a targeting moiety.
Haralambidis
J
J
Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne, Parkville, Victoria, Australia.
Duncan
L
L
Angus
K
K
Tregear
G W
GW
D000595:Amino Acid Sequence; D015119:Aminocaproic Acid; D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D015342:DNA Probes / Q000138:chemical synthesis; D006880:Hydroxy Acids; D008969:Molecular Sequence Data; D009757:Nylons / Q000138:chemical synthesis*; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*
D015342:DNA Probes; D006880:Hydroxy Acids; D009757:Nylons; D009838:Oligodeoxyribonucleotides; D015119:Aminocaproic Acid