High-performance liquid chromatographic determination of beta-phenylethylamine in human plasma with fluorescence detection.
A simple and highly sensitive method for the determination of beta-phenylethylamine in human plasma is investigated. The method employs high-performance liquid chromatography with fluorescence detection. beta-Phenylethylamine and p-methylbenzylamine (internal standard) in human plasma are isolated by cation-exchange chromatography on a Toyopak SP cartridge and then converted into the corresponding fluorescent derivatives with 3,4-dihydro-6,7-dimethoxy-4-methyl-3-oxoquinoxaline-2-carbonyl chloride, a fluorescence derivatization reagent for amines. The derivatives are separated within 30 min on a reversed-phase column, TSK gel ODS-120T, with isocratic elution, and detected fluorometrically. The detection limit of beta-phenylethylamine is 0.3 pmol/ml in plasma (S/N = 3).
Ishida
J
J
Faculty of Pharmaceutical Sciences, Fukuoka University, Japan.
Yamaguchi
M
M
Nakamura
M
M
D001596:Benzylamines; D002851:Chromatography, High Pressure Liquid; D005453:Fluorescence; D006801:Humans; D010627:Phenethylamines / Q000097:blood*; D011810:Quinoxalines; D012015:Reference Standards; D012044:Regression Analysis
D001596:Benzylamines; D010627:Phenethylamines; D011810:Quinoxalines; C063400:4-methylbenzylamine; C063399:3,4-dihydro-6,7-dimethoxy-4-methyl-3-oxoquinoxaline-2-carbonyl chloride; C029261:phenethylamine