title: Characterization of monoclonal antibodies reactive with normal resting, lactating and neoplastic human breast.
abstract: Mouse monoclonal antibodies reacting with human mammary gland constituents have been generated and characterized in an attempt to raise breast- or breast-cancer-specific antisera. The immunogens used in these studies included fractionated human milk fat globule membrane, the human breast cancer cell line MCF7 and a crude membrane preparation derived from an axillary nodal metastasis from a patient with breast cancer. Of the antibodies obtained, 8 were characterized and found to bind to different structures in the normal breast. The antibody LICR-LON-LC28 recognizes secretory component and binds strongly to normal resting and lactating breast, but only focally to a minority of breast carcinomas. The antibodies LICR-LON-14.1 and 32.2 react strongly with the lactating breast and recognize kappa- and beta-casein, respectively. Caseins are not produced by breast tumors. The antibodies LICR-LON-TW19.5, H10A and 39.8 all react with carbohydrate epitopes and bind heterogeneously to normal resting breast luminal epithelium and cellular subsets of breast carcinomas. LICR-LON-59.2 and 19.2 react with normal breast myoepithelial cells and the basement membrane, respectively. LICR-LON-59.2 is unusual as a myoepithelial marker in that it stains cells in the majority of breast carcinomas. LICR-LON-19.2 shows extensive reactivity to tumor cell lines in culture but has no reactivity with carcinoma cells from breast biopsies.
authors.affiliation: Ludwig Institute for Cancer Research, Sutton, Surrey, UK.
authors.forename: R A
authors.initials: RA
authors.lastname: Skilton
authors.forename: H M
authors.initials: HM
authors.lastname: Earl
authors.forename: M E
authors.initials: ME
authors.lastname: Gore
authors.forename: R A
authors.initials: RA
authors.lastname: McIlhinney
authors.forename: B A
authors.initials: BA
authors.lastname: Gusterson
authors.forename: P
authors.initials: P
authors.lastname: Wilson
authors.forename: R C
authors.initials: RC
authors.lastname: Coombes
authors.forename: A M
authors.initials: AM
authors.lastname: Neville
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D015153:Blotting, Western; D001940:Breast / Q000166:cytology / Q000276:immunology*; D001943:Breast Neoplasms / Q000276:immunology*; D002364:Caseins / Q000276:immunology; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D002846:Chromatography, Affinity; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007774:Lactation; D037102:Lectins; D051379:Mice; D008807:Mice, Inbred BALB C; D009362:Neoplasm Metastasis / Q000276:immunology; D017830:Octoxynol; D011092:Polyethylene Glycols; D011247:Pregnancy; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D002364:Caseins; D037102:Lectins; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40