Expression of the beta-glucuronidase gene under the control of the CaMV 35s promoter in Schizosaccharomyces pombe.
We have transformed Schizosaccharomyces pombe with the beta-glucuronidase (GUS) gene from Escherichia coli under the control of the plant cauliflower mosaic virus (CaMV) 35S promoter element. Efficient expression of GUS enzyme was observed. Moreover, transcription initiated at a unique site identical to that used in plant cells.
Pobjecky
N
N
Department of Bioscience and Biotechnology, Drexel University, Philadelphia, PA 19104.
Rosenberg
G H
GH
Dinter-Gottlieb
G
G
Käufer
N F
NF
D001483:Base Sequence; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D005966:Glucuronidase / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D004718:Saccharomycetales / Q000235:genetics*; D012568:Schizosaccharomyces / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D005966:Glucuronidase