title: Cloning and analysis of a 35.3-kilobase DNA region involved in exopolysaccharide production by Xanthomonas campestris pv. campestris.
abstract: Cosmid clones able to restore exopolysaccharide production in possibly insertion sequence element-induced surface mutants of Xanthomonas campestris pv. campestris were isolated. By fragment-specific Tn5-lac mutagenesis of one of the cosmids, pXCB1002, a new DNA region which is involved in exopolysaccharide biosynthesis and which is organized into at least 12 complementation groups was identified.
authors.affiliation: Lehrstuhl für Genetik, Fakultät für Biologie, Universität Bielefeld, Federal Republic of Germany.
authors.forename: B
authors.initials: B
authors.lastname: Hötte
authors.forename: I
authors.initials: I
authors.lastname: Rath-Arnold
authors.forename: A
authors.initials: A
authors.lastname: Pühler
authors.forename: R
authors.initials: R
authors.lastname: Simon
mesh_terms: D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003360:Cosmids; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D015723:Gene Library; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D010641:Phenotype; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D014974:Xanthomonas / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D011135:Polysaccharides, Bacterial