Method for radio-capillary gas chromatography employing a modified oxidation-reduction train and flow-through detector.
A conventional radioactivity monitor was modified by reducing the volume of the oxidation-reduction train and gas proportional counting tube to permit coupled radio-capillary gas chromatographic analysis of labeled isomeric metabolites. The utility of this radioactivity monitoring technique was demonstrated by separating 3H-labeled sesquiterpene olefins. The advantages and limitations of mass (thermal conductivity) and radioactivity detection by this method are discussed.
Croteau
R B
RB
Institute of Biological Chemistry, Washington State University, Pullman 99164-6340.
Satterwhite
D M
DM
D002849:Chromatography, Gas / Q000295:instrumentation*; D012717:Sesquiterpenes / Q000032:analysis; D014316:Tritium / Q000032:analysis*
D012717:Sesquiterpenes; D014316:Tritium