[Contribution of enzyme-linked immuno-electrotransfer blot assay for the diagnosis of human neurocysticercosis].
An enzyme-linked immunoelectrotransfer blot (EITB) is developed for immunodiagnosing human neurocysticercosis. The assay's efficacy is evaluated in 71 neurocysticercotic patients whose diagnosis of the disease and evaluation of disease activity is based on CT scan findings. We identified eight bands (molecular weigh less than 35 000) that evaluate the disease activity: active disease, calcification in progress, inactive disease. All samples patients with active disease recognized 14 000 band. This band is missing in the others parasitic diseases and in serum of the patients with inactive diseases or calcification in progress. The enzyme-linked immunoelectrotransfer blot is simple to perform and may be used in sero-epidemiology.
Michault
A
A
Service de Bactériologie-Parasitologie, Centre Hospitalier Général Saint-Pierre.
Rivière
B
B
Fressy
P
P
Laporte
J P
JP
Bertil
G
G
Mignard
C
C
D003429:Cross Reactions; D003551:Cysticercosis / Q000145:classification / Q000175:diagnosis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting; D014057:Tomography, X-Ray Computed
Apport de l'enzyme-linked immunoelectrotransfer blot assay au diagnostic de la neurocysticercose humaine.