1H NMR observation of tissue myoglobin: an indicator of cellular oxygenation in vivo.
Cellular oxygen status is a critical parameter. To measure the oxygen tension in vivo, however, demands extant techniques to surmount two experimental difficulties: invasive experimental protocols and imprecise tissue localization. NMR techniques have the potential to overcome these obstacles. Indeed we show in this study that cellular myoglobin is NMR visible and its proximal histidyl NH signal in the deoxy state can be used to monitor changes in cellular oxygenation. Coupled with recent advances in NMR signal localization, this 1H NMR strategy promises to lead directly to cellular oxygen measurement in specific tissue regions in vivo.
Jue
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T
Department of Biological Chemistry, University of California, Davis 95616.
Anderson
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S
D000818:Animals; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009206:Myocardium / Q000032:analysis* / Q000378:metabolism; D009211:Myoglobin / Q000032:analysis*; D010101:Oxygen Consumption; D010477:Perfusion / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains
D009211:Myoglobin; D006859:Hydrogen