title: In vitro transcription of the double-stranded RNA genome of maize rough dwarf virus (Reoviridae).
abstract: An RNA-dependent RNA polymerase associated with particles of maize rough dwarf virus, a Fijivirus, was characterized using two in vitro assays differing in their energy regeneration systems. Optimum reaction rates occurred at pH 8.0 to 8.5 at 20 degrees C. The presence of virus and Mn2+ or Mg2+ was essential for enzyme activity; Mn2+ stimulated more incorporation events than Mg2+, at optimum concentrations of 2 to 4 mM and 4 mM, respectively. Incorporation was not affected by alpha-amanitin, actinomycin D or rifampicin. The products synthesized in vitro were single-stranded RNAs which hybridized specifically with the double-stranded genomic RNAs of five other reoviruses. The in vitro transcripts were also used to detect maize rough dwarf virus RNA in plants and in vector insects.
authors.affiliation: Istituto di Fitovirologia applicata del C.N.R., Turin, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Marzachì
authors.forename: G P
authors.initials: GP
authors.lastname: Accotto
authors.forename: M
authors.initials: M
authors.lastname: d'Aquilio
authors.forename: P
authors.initials: P
authors.lastname: Caciagli
authors.forename: G
authors.initials: G
authors.lastname: Boccardo
mesh_terms: D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005814:Genes, Viral; D007700:Kinetics; D012367:RNA, Viral / Q000235:genetics*; D012087:Reoviridae / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic; D003313:Zea mays / Q000382:microbiology
chemical_list: D012367:RNA, Viral; D012321:DNA-Directed RNA Polymerases