SshAI restriction endonuclease from Salmonella shikmonah.
A new type II restriction endonuclease, SshAI, was purified from Salmonella shikmonah TK139 of kangaroo origin. The recognition and cleavage specificity of Ssh AI was determined to be 5'-CC/TNAGG-3', identical to that of SauI from Streptomyces aureofaciens and Bsu36I from Bacillus subtilis. Based on closely related and in part overlapping recognition specificities of Ssh AI and other restriction endonucleases, a close evolutionary relationship is proposed for all known Salmonella restriction endonucleases.
Miyahara
M
M
Division of Microbiology, National Institute of Hygienic Sciences, Tokyo, Japan.
Nakajima
K
K
Kawanishi
T
T
Mise
K
K
D000818:Animals; D001483:Base Sequence; D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics / Q000302:isolation & purification*; D005516:Food Microbiology; D007614:Macropodidae; D008460:Meat; D012475:Salmonella / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D013379:Substrate Specificity
D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific; C062194:endodeoxyribonuclease SauI