title: Onchocerca raillieti: release from skin snips, maintenance in vitro and periodicity of microfilariae.
abstract: Several media were tested for the release of Onchocerca raillieti microfilariae from skin snips and for their subsequent in vitro maintenance. Tyrode's solution containing 20% equine serum and antibiotics was the best medium tested, followed by phosphate buffered saline. Tyrode's solution alone or distilled water were poor media. A temperature 7-12 degrees C lower than the host's body temperature favoured release of the microfilariae from skin snips. The microfilariae were best maintained at 4-10 degrees C, when they remained alive for up to 5 days. O. raillieti microfilariae had an evening periodicity which could be related to a possible vector's peak of feeding activity.
authors.affiliation: Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors.forename: H S
authors.initials: HS
authors.lastname: Hussein
authors.forename: S E
authors.initials: SE
authors.lastname: el Sammani
mesh_terms: D000818:Animals; D003470:Culture Media; D008842:Microfilariae / Q000502:physiology; D009854:Onchocerca / Q000254:growth & development / Q000502:physiology*; D009855:Onchocerciasis / Q000469:parasitology / Q000662:veterinary*; D010507:Periodicity; D010527:Perissodactyla / Q000469:parasitology*; D012621:Seasons; D012867:Skin / Q000469:parasitology*; D013696:Temperature
chemical_list: D003470:Culture Media