title: Developmentally regulated transporter in Leishmania is encoded by a family of clustered genes.
abstract: We have previously cloned a gene for a developmentally regulated transport protein from the trypanosomatid protozoan Leishmania enriettii. We demonstrate here that this transporter is encoded by a single family of tandemly clustered genes containing approximately 8 copies of the 3.6 kilobase repeat unit. Transcriptional mapping defines a contiguous 3.3 kilobase region of the repeat unit that encodes the mRNA. The 5' end of the mature mRNA contains the spliced leader or mini-exon previously identified in kinetoplastid protozoa, while the 3' ends of the mRNA are heterogeneous in sequence and in location of the polyadenylation site. We have identified genomic restriction fragments that flank the tandem repeat on the 5' and 3' sides and which may be linked to sequences required for expression of the gene family. Other species of Leishmania also contain sequences that hybridize to the cloned L. enriettii gene at high stringency.
authors.affiliation: Department of Microbiology and Immunology, Oregon Health Sciences University, Portland 97201.
authors.forename: D A
authors.initials: DA
authors.lastname: Stein
authors.forename: B R
authors.initials: BR
authors.lastname: Cairns
authors.forename: S M
authors.initials: SM
authors.lastname: Landfear
mesh_terms: D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D005786:Gene Expression Regulation; D015723:Gene Library; D007891:Leishmania / Q000235:genetics* / Q000254:growth & development; D008969:Molecular Sequence Data; D005810:Multigene Family; D021382:Protein Sorting Signals / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D002352:Carrier Proteins; D021382:Protein Sorting Signals; D012333:RNA, Messenger