Differential effects of the protein kinase inhibitor K-252a on the in vitro survival of chick embryonic neurons.
The effects of the protein kinase inhibitor K-252a on the in vitro survival of different populations of chick embryonic neurons were tested. Following dissociation from the corresponding ganglia by trituration, the neurons were cultured on laminin-coated dishes in both the presence and absence of their respective neurotrophic factor. K-252a alone promoted long-term neuronal survival of dorsal root ganglion (DRG) and ciliary ganglion (CG) neurons in a dose-dependent fashion. No such effect was seen with sympathetic ganglion (SG) neurons. In addition, K-252a did not interfere with nerve growth factor (NGF)- or ciliary neuronotrophic factor (CNTF)-mediated survival of DRG or CG neurons, respectively, but completely blocked the NGF- and CNTF-induced survival of SG neurons. High potassium-induced survival of SG neurons was not affected by K-252a. These results point to differences between various neuronal populations in the signal transduction mechanism for neurotrophic factors.
Borasio
G D
GD
Max-Planck-Institute for Psychiatry, Department of Neurochemistry, Martinsried, F.R.G.
D000818:Animals; D002227:Carbazoles / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005726:Ganglia, Parasympathetic / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D005728:Ganglia, Sympathetic / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D026121:Indole Alkaloids; D011494:Protein Kinases / Q000378:metabolism*
D002227:Carbazoles; D004791:Enzyme Inhibitors; D026121:Indole Alkaloids; C049985:staurosporine aglycone; D011494:Protein Kinases