High-performance liquid chromatographic analysis of catecholamines in biological samples by liquid/liquid extraction prepurification.
A prepurification procedure for the determination of catecholamines in biological samples was studied to simplify the procedure and increase selectivity and recovery. The procedure is based on liquid/liquid extraction of catecholamine-borate complexes. Under optimal conditions, catecholamines formed complexes with diphenylborate in an alkaline NH4Cl/NH4OH buffer that were extracted with n-heptanol. Catecholamines were reextracted into an HCl-acidified solution, followed by reversed-phase ion-pair high-performance liquid chromatographic separation with fluorometric detection. Application of the method to various samples of rat tissues and human urine showed procedural simplicity, high selectivity, and high recovery (about 90% or more for tissue samples). This method can be used for pharmacological studies on catecholamines.
Tsuchiya
H
H
Department of Dental Pharmacology, Asahi University School of Dentistry, Gifu, Japan.
Hayashi
T
T
D000328:Adult; D000818:Animals; D001896:Boron Compounds / Q000032:analysis / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004298:Dopamine / Q000032:analysis* / Q000652:urine; D004837:Epinephrine / Q000032:analysis* / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D009638:Norepinephrine / Q000032:analysis* / Q000652:urine; D051381:Rats; D011919:Rats, Inbred Strains
D001896:Boron Compounds; C035757:diphenylborate; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine