Defective presentation of endogenous antigen by a cell line expressing class I molecules.
Cytotoxic T lymphocytes (CTLs) recognize class I major histocompatibility complex (MHC) molecules associated with antigenic peptides derived from endogenously synthesized proteins. Binding to such peptides is a requirement for class I assembly in the endoplasmic reticulum (ER). A mutant human cell line, T2, assembles and transports to its surface some, but not all, class I MHC molecules. The class I molecules expressed on the surface of T2 do not present peptides derived from cytosolic antigens, although they can present exogenously added peptides to CTL. The transported class I molecules may interact weakly with an unknown retaining factor in the ER such that they can assemble despite the relative shortage of peptides.
Hosken
N A
NA
Department of Immunology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
Bevan
M J
MJ
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000941:Antigens / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D002213:Capsid / Q000276:immunology; D002460:Cell Line; D004721:Endoplasmic Reticulum / Q000276:immunology; D015870:Gene Expression; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology; D006680:HLA Antigens / Q000235:genetics; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D006801:Humans; D051379:Mice; D009154:Mutation; D010047:Ovalbumin / Q000276:immunology; D010455:Peptides / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014162:Transfection; D014407:Tumor Cells, Cultured; D014758:Viral Core Proteins / Q000276:immunology
D000941:Antigens; D000956:Antigens, Viral; D006183:H-2 Antigens; D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D010455:Peptides; D014758:Viral Core Proteins; D010047:Ovalbumin