Synergistic induction of cytogenetic damage by the homo-aza-steroidal ester of p-bis(2-chloroethyl)aminophenylacetic acid in combination with caffeine in human lymphocytes in vitro and in Ehrlich ascites tumour cells in vivo.
We studied the effects of caffeine alone or in combination with homo-aza-steroidal ester of p-bis(2-chloroethyl)aminophenylacetic acid (ASE, NSC 290205) on the frequency of SCEs and lymphocyte proliferation kinetics. Caffeine was found to act synergistically with ASE on the induction of SCEs when the two components were administered in combination. Caffeine was also found to act synergistically with ASE in inducing cell-division delays. Enhanced cytogenetic damage by ASE was observed when Ehrlich ascites tumour cells (EAT cells) were exposed in vivo to caffeine. ASE alone or in combination with caffeine caused a dose-dependent increase in SCE rates and cell-division delays. SCEs were demonstrated in EAT-bearing mice, by the i.p. injection of BrdUrd adsorbed onto activated charcoal, 1 h after the i.p. injection of ASE and/or caffeine.
Petrou
C
C
Department of Medical Biology and Genetics, Faculty of Medicine, Aristotelian University, Thessaloniki, Greece.
Mourelatos
D
D
Dozi-Vassiliades
J
J
Catsoulacos
P
P
D000818:Animals; D002110:Caffeine / Q000633:toxicity*; D002286:Carcinoma, Ehrlich Tumor; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016009:Chi-Square Distribution; D004357:Drug Synergism; D004952:Esters; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D009153:Mutagens; D009588:Nitrogen Mustard Compounds / Q000633:toxicity*; D012854:Sister Chromatid Exchange / Q000187:drug effects; D014407:Tumor Cells, Cultured
D004952:Esters; D009153:Mutagens; D009588:Nitrogen Mustard Compounds; C008826:phenacid; D002110:Caffeine