Lipoprotein lipase mRNA expression in brown adipose tissue: translational and/or posttranslational events are involved in the modulation of enzyme activity.
Lipoprotein lipase mRNA abundance in rat brown adipose tissue increases during the first 24 h of cold exposure. Lipoprotein lipase mRNA levels do not change in brown fat throughout pregnancy and lactation, whereas enzyme activity is significantly lowered. After 5 h of acute cold or noradrenaline administration there is a 2-fold increase in lipoprotein lipase mRNA abundance, whereas lipoprotein lipase activity is stimulated to more than 6-fold the basal values. It is concluded that translational and/or posttranslational mechanisms are involved in the noradrenergic modulation of lipoprotein lipase activity in brown fat.
Giralt
M
M
Unitat de Bioquímica i Biologia Molecular B, Universitat de Barcelona, Spain.
Martin
I
I
Vilaró
S
S
Villarroya
F
F
Mampel
T
T
Iglesias
R
R
Viñas
O
O
D000064:Acclimatization; D002001:Adipose Tissue, Brown / Q000187:drug effects / Q000201:enzymology*; D000818:Animals; D015152:Blotting, Northern; D003080:Cold Temperature; D005260:Female; D015870:Gene Expression; D006801:Humans; D007774:Lactation; D008071:Lipoprotein Lipase / Q000235:genetics* / Q000378:metabolism; D009638:Norepinephrine / Q000494:pharmacology; D011247:Pregnancy; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values
D012333:RNA, Messenger; D008071:Lipoprotein Lipase; D009638:Norepinephrine