title: Lipoprotein lipase mRNA expression in brown adipose tissue: translational and/or posttranslational events are involved in the modulation of enzyme activity.
abstract: Lipoprotein lipase mRNA abundance in rat brown adipose tissue increases during the first 24 h of cold exposure. Lipoprotein lipase mRNA levels do not change in brown fat throughout pregnancy and lactation, whereas enzyme activity is significantly lowered. After 5 h of acute cold or noradrenaline administration there is a 2-fold increase in lipoprotein lipase mRNA abundance, whereas lipoprotein lipase activity is stimulated to more than 6-fold the basal values. It is concluded that translational and/or posttranslational mechanisms are involved in the noradrenergic modulation of lipoprotein lipase activity in brown fat.
authors.affiliation: Unitat de Bioquímica i Biologia Molecular B, Universitat de Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Giralt
authors.forename: I
authors.initials: I
authors.lastname: Martin
authors.forename: S
authors.initials: S
authors.lastname: Vilaró
authors.forename: F
authors.initials: F
authors.lastname: Villarroya
authors.forename: T
authors.initials: T
authors.lastname: Mampel
authors.forename: R
authors.initials: R
authors.lastname: Iglesias
authors.forename: O
authors.initials: O
authors.lastname: Viñas
mesh_terms: D000064:Acclimatization; D002001:Adipose Tissue, Brown / Q000187:drug effects / Q000201:enzymology*; D000818:Animals; D015152:Blotting, Northern; D003080:Cold Temperature; D005260:Female; D015870:Gene Expression; D006801:Humans; D007774:Lactation; D008071:Lipoprotein Lipase / Q000235:genetics* / Q000378:metabolism; D009638:Norepinephrine / Q000494:pharmacology; D011247:Pregnancy; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values
chemical_list: D012333:RNA, Messenger; D008071:Lipoprotein Lipase; D009638:Norepinephrine