Virosomes reconstituted from human immunodeficiency virus proteins and lipids.
Purified Human Immunodeficiency Virus (HIV) was solubilized in octylglucopyranoside. After centrifugation, the supernatant was added to lipid-detergent mixed micelles. Formation of virosomes occurred during overnight dialysis. Centrifugation on a continuous glycerol gradient showed that envelope glycoproteins (gp120 and gp41) and matrix protein p17 but not core protein p25 were associated to virosomes. Proteolytic treatment of virosomes indicates that gp120 is oriented toward the outside as in the virus particles, whereas p17 protein is anchored on both sides of the liposomal membrane. Virosomes are spherical vesicles with approximately the size of the virus as shown by electron microscopy.
Cornet
B
B
Laboratoire de Chimie-Physique des Macromolécules aux Interfaces, Université Libre de Bruxelles, Belgium.
Vandenbranden
M
M
Cogniaux
J
J
Giurgea
L
L
Dekegel
D
D
Ruysschaert
J M
JM
D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D006678:HIV / Q000302:isolation & purification / Q000648:ultrastructure; D006801:Humans; D006868:Hydrolysis; D007202:Indicators and Reagents; D008055:Lipids; D008854:Microscopy, Electron; D013501:Surface-Active Agents; D014357:Trypsin; D014764:Viral Proteins
D007202:Indicators and Reagents; D008055:Lipids; D013501:Surface-Active Agents; D014764:Viral Proteins; D014357:Trypsin