Size of the Streptococcus mutans GS-5 chromosome as determined by pulsed-field gel electrophoresis.
Rare cutting restriction endonucleases were used to cut the Streptococcus mutans chromosome into large fragments. Restriction enzymes utilizing recognition sites containing 6-, 7-, or 8-base-pair sequences with only G and C nucleotides produced few fragments, most of which were greater than 100 kilobase pairs in size. Addition of the fragments from digests of SmaI, NotI, ApaI, RsrII, and EagI yielded a molecular size for the S. mutans GS-5 genome of 2,819 +/- 60 kilobase pairs.
Tudor
J J
JJ
Biology Department, Saint Joseph's University, Philadelphia, Pennsylvania 19131.
Marri
L
L
Piggot
P J
PJ
Daneo-Moore
L
L
D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000032:analysis; D004586:Electrophoresis; D013295:Streptococcus mutans / Q000235:genetics*
D004269:DNA, Bacterial