DNA repair and chromosomal stability in the alkylating agent-hypersensitive Chinese hamster cell line 27-1.
27-1 is a mutant of Chinese hamster ovary cells (CHO cells) that is hypersensitive to the toxic effects of ultraviolet light, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and other monofunctional alkylating agents. We show here that the enhanced MNNG sensitivity of these cells is not due to alterations in the amount of DNA methylation products introduced nor by a defect in the first step of removal of the main alkylation products 7-methylguanine and 3-methyladenine. However, these mutant cells perform more DNA repair synthesis after treatment with MNNG than normal CHO-9 cells. This observation might indicate a possible defect of a ligase involved in sealing DNA repair patches. 27-1 cells did not show elevated frequencies of sister-chromatid exchange and chromosomal aberration induced by MNNG. The data show that MNNG-induced cell killing is not necessarily related to increased chromosomal instability.
Kaina
B
B
Department of Genetics and Toxicology, Nuclear Research Center, Karlsruhe, F.R.G.
van Zeeland
A A
AA
de Groot
A
A
Natarajan
A T
AT
D000477:Alkylating Agents / Q000633:toxicity*; D000818:Animals; D002460:Cell Line / Q000187:drug effects* / Q000648:ultrastructure; D002875:Chromosomes / Q000187:drug effects*; D006224:Cricetinae; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D004260:DNA Repair / Q000187:drug effects*; D008769:Methylnitronitrosoguanidine / Q000633:toxicity*; D009153:Mutagens; D012854:Sister Chromatid Exchange / Q000187:drug effects
D000477:Alkylating Agents; D009153:Mutagens; D008769:Methylnitronitrosoguanidine; D004247:DNA