title: Expanded HIV-1 cellular tropism by phenotypic mixing with murine endogenous retroviruses.
abstract: In view of the current interest in in vivo murine models for acquired immunodeficiency syndrome (AIDS), the interaction between human immunodeficiency virus type 1 (HIV-1) and endogenous murine leukemia virus (MuLV)-related retroviruses was investigated with a human leukemic T cell line (PF-382x) that acquired xenotropic MuLV (X-MuLV) after in vivo passage in immunosuppressed mice. Despite similar levels of membrane CD4 expression and HIV-1 125I-labeled gp 120 binding, a dramatic acceleration in the time course of HIV-1 infection was observed in PF-382x compared to its X-MuLV-negative counterpart (PF-382). Moreover, PF-382 cells coinfected by X-MuLV and HIV-1 generated a progeny of phenotypically mixed viral particles, enabling HIV-1 to productively infect a panel of CD4- human cells, including B lymphoid cells and purified normal peripheral blood CD4-/CD8+ T lymphocytes. Mixed viral phenotypes were also produced by human CD4+ T cells coinfected with an amphotropic MuLV-related retrovirus (A-MuLV) and HIV-1. These data show that endogenous MuLV acquired by human cells transplanted into mice can significantly interact with HIV-1, thereby inducing important alterations of HIV-1 biological properties.
authors.affiliation: Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, MD 20892.
authors.forename: P
authors.initials: P
authors.lastname: Lusso
authors.forename: F
authors.initials: F
authors.lastname: di Marzo Veronese
authors.forename: B
authors.initials: B
authors.lastname: Ensoli
authors.forename: G
authors.initials: G
authors.lastname: Franchini
authors.forename: C
authors.initials: C
authors.lastname: Jemma
authors.forename: S E
authors.initials: SE
authors.lastname: DeRocco
authors.forename: V S
authors.initials: VS
authors.lastname: Kalyanaraman
authors.forename: R C
authors.initials: RC
authors.lastname: Gallo
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal; D015704:CD4 Antigens / Q000032:analysis; D002460:Cell Line; D002472:Cell Transformation, Viral; D004195:Disease Models, Animal; D015497:HIV-1 / Q000235:genetics* / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000382:microbiology; D006801:Humans; D051379:Mice; D010641:Phenotype; D012190:Retroviridae / Q000235:genetics*; D014764:Viral Proteins / Q000032:analysis; D014779:Virus Replication
chemical_list: D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D014764:Viral Proteins