title: Ethanol treatment induces a delayed segmentation anomaly in the chick embryo.
abstract: A repeatable somite anomaly is described that results from the incubation of cultured chick embryos in the presence of ethanol. The anomaly comprises a misalignment of approximately five consecutive pairs of somites such that one of each pair is displaced cranially by up to one-half a somite length. The appearance of the malformation is delayed by approximately six somite pairs after the beginning of treatment. These characteristics were shared by embryos treated at the stage of gastrulation (no somites yet present) up to embryos possessing ten pairs of somites at treatment time. The deleterious effect did not appear to result from a disruption in the mechanics of the segmentation process itself, since isolated segmental plates were able to form normal intersomitic clefts in the presence of ethanol. Similarly, there were apparently no alterations in the compaction process that occurs at the cranial end of the segmental plate, since both the contractile and adhesive components were unaffected, as judged by the distributions of actin and fibronectin. The potential mechanisms of the anomaly are discussed with reference to similar segmental defects produced by heat shock. In view of earlier results indicating that cells in the primitive streak at gastrulation are sensitive to the presence of ethanol, it is proposed that this somite anomaly is due to a disruption in the contribution of these mesoderm cells to the segmental plate.
authors.affiliation: Department of Physiology, University of Alberta, Edmonton, Canada.
authors.forename: E J
authors.initials: EJ
authors.lastname: Sanders
authors.forename: E
authors.initials: E
authors.lastname: Cheung
mesh_terms: D000014:Abnormalities, Drug-Induced / Q000196:embryology / Q000378:metabolism*; D000199:Actins / Q000378:metabolism; D000818:Animals; D002642:Chick Embryo; D046508:Culture Techniques; D000431:Ethanol / Q000633:toxicity*; D005353:Fibronectins / Q000378:metabolism; D008648:Mesoderm / Q000187:drug effects*; D013997:Time Factors
chemical_list: D000199:Actins; D005353:Fibronectins; D000431:Ethanol