Expression of the gene for anti-Müllerian hormone.
The expression of anti-Müllerian hormone, also called Müllerian-inhibiting substance or factor, is limited to immature Sertoli and post-natal granulosa cells. To gain information on the genetic mechanisms of this tissue- and development-specific regulation, a human genomic clone containing 3.6 kilobases of 5' flanking sequences has been isolated and sequenced. Transcription initiation sites include a major site, at the same position as in the bovine gene, and 3 minor sites, accounting together for 12% of transcripts. Sequences of potential interest include an Sp1 binding site, minisatellite-like regions, and a 13-base pair palindrome, nearly identical to the oestrogen response element of the vitellogenin genes. This palindrome binds to the human oestrogen receptor in vitro and activates transcription of a hybrid gene when several copies are inserted 5' of a thymidine kinase promoter. Further studies are needed to determine whether this palindrome is implicated in a feed-back loop regulating oestrogen synthesis in granulosa cells.
Guerrier
D
D
Unité de Recherches sur l'Endocrinologie du Développement, INSERM, Montrouge, France.
Boussin
L
L
Mader
S
S
Josso
N
N
Kahn
A
A
Picard
J Y
JY
D054304:Anti-Mullerian Hormone; D001483:Base Sequence; D015870:Gene Expression / Q000502:physiology*; D006023:Glycoproteins; D006131:Growth Inhibitors / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009095:Mullerian Ducts / Q000502:physiology*; D011960:Receptors, Estrogen / Q000378:metabolism; D013735:Testicular Hormones / Q000235:genetics*; D014158:Transcription, Genetic / Q000502:physiology
D006023:Glycoproteins; D006131:Growth Inhibitors; D011960:Receptors, Estrogen; D013735:Testicular Hormones; D054304:Anti-Mullerian Hormone