title: Viral integration, fragile sites, and proto-oncogenes in human neoplasia.
abstract: To evaluate the trend of viral integration in the human genome, chromosomal localization of five DNA-containing viruses compiled from literature data was compared to the location of fragile sites and proto-oncogenes. A total of 35 regionally mapped viral integration sites from tumors and transformed cells were distributed over 19 chromosomes. Of the 35 integration sites 23 (66%) were at the bands of fragile sites, and 7 were one band away (20%). This statistically defines the correlation as highly significant (P = 0.0000183, Fisher's F-test). Five integration sites did not correspond to the location of a fragile site. Thirteen integration sites and proto-oncogenes mapped at the same bands (37%), 6 (17%) were one band apart, and at 16 integration sites (46%) no proto-oncogenes were localized (P = 0.00491). Eighteen viral integration sites, fragile sites, and proto-oncogenes (51%) were localized at the same bands or one band distant. This clustering of viral integration sites, fragile sites, and proto-oncogenes is statistically highly significant (P = 0.0000118), and indicates nonrandom viral integration in the human genome.
authors.affiliation: Division of Cancer Etiology, National Cancer Institute, Bethesda, MD 20892.
authors.forename: N C
authors.initials: NC
authors.lastname: Popescu
authors.forename: D
authors.initials: D
authors.lastname: Zimonjic
authors.forename: J A
authors.initials: JA
authors.lastname: DiPaolo
mesh_terms: D043283:Chromosome Fragile Sites; D002873:Chromosome Fragility; D004279:DNA, Viral / Q000235:genetics; D006801:Humans; D009369:Neoplasms / Q000235:genetics* / Q000382:microbiology; D009858:Oncogenic Viruses / Q000235:genetics*; D011519:Proto-Oncogenes; D011995:Recombination, Genetic; D014412:Tumor Virus Infections / Q000235:genetics*
chemical_list: D004279:DNA, Viral