Visualization of chitin-protein layer formation in Ascaris lumbricoides egg-shells.
The formation of chitin-protein layer in Ascaris lumbricoides egg-shells was studied using the method of chitin structures visualization by means of derivatives of stilbene-disulfonic acid (Blankophor, Bayer, FRG). Intensive formation of chitin structures in A. lumbricoides egg-shell occurred only in fertilized females in a very short portion of uterus from the site of connection of the oviduct with the uterus up to 25-30 mm distally, i.e. in the section forming about one fifth or less than one sixth of length of adult female uterus. Already 3 mm from the beginning of uterus there was a thin layer on the surface of the fertilized eggs which contained chitin detectable by Blankophor visualization, whereas the chitin-protein layer on the shells could not be demonstrated histologically. The thickness of this chitin-protein complex increased distally. At 25 mm from the beginning of uterus the chitin layer was already complete and its thickness did not increase any more.
Sromová
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Department of Medical Biology, Medical Faculty, Palacký University, Olomouc, Czechoslovakia.
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D000818:Animals; D001200:Ascaris / Q000033:anatomy & histology*; D002686:Chitin / Q000032:analysis* / Q000096:biosynthesis; D005260:Female; D005456:Fluorescent Dyes; D015801:Helminth Proteins / Q000032:analysis* / Q000096:biosynthesis; D006651:Histocytochemistry; D008856:Microscopy, Fluorescence; D009930:Organic Chemicals; D010063:Ovum / Q000648:ultrastructure*
D005456:Fluorescent Dyes; D015801:Helminth Proteins; D009930:Organic Chemicals; C055193:Blankophor BA; D002686:Chitin