A simple method for isolation of DNA fragments associated with the nuclear lamina in vivo.
We describe a simple method for the purification of DNA fragments associated with the nuclear lamina in vivo. Ehrlich ascite tumor cells are first u.v.-irradiated to crosslink DNA to proteins. The nuclear lamina is then isolated and purified by low-speed centrifugation through a cushion of 40% sucrose. The material sedimenting through the created density barrier represents nuclear lamina of a very high purity, free from any DNA fragments except those which were in a crosslinking distance to it in vivo.
Christova
R
R
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
Galcheva-Gargova
Z
Z
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000235:genetics; D002501:Centrifugation, Zonal; D004247:DNA / Q000302:isolation & purification*; D004273:DNA, Neoplasm / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D051379:Mice; D015530:Nuclear Matrix / Q000032:analysis* / Q000648:ultrastructure; D009687:Nuclear Proteins / Q000302:isolation & purification; D014466:Ultraviolet Rays
D004273:DNA, Neoplasm; D009687:Nuclear Proteins; D004247:DNA