Direct nonradioactive in situ hybridization of somatic cell hybrid DNA to human lymphocyte chromosomes.
Biotinylated DNA from various human-rodent hybrids was hybridized to human lymphocyte spreads after preannealing of the repeated sequences with sonicated total human DNA. Fluorescent labeling was achieved by successive treatments with fluorescein-labeled avidin and biotinylated antiavidin antibody. The use of labeled total DNA from hybrids with known chromosome composition permits the fluorescent staining-("painting") of specific chromosomes, or parts thereof, in human lymphocyte metaphases. Alternatively, the human chromosome content of cell hybrids with unknown chromosome composition is directly assessed from the labeling pattern of human lymphocyte spreads using the total hybrid DNA as probe.
Kievits
T
T
Department of Human Genetics, University of Leiden, The Netherlands.
Devilee
P
P
Wiegant
J
J
Wapenaar
M C
MC
Cornelisse
C J
CJ
van Ommen
G J
GJ
Pearson
P L
PL
D000818:Animals; D002875:Chromosomes; D003582:Cytogenetics / Q000379:methods*; D015342:DNA Probes; D005456:Fluorescent Dyes; D006801:Humans; D006822:Hybrid Cells; D008214:Lymphocytes / Q000648:ultrastructure; D008677:Metaphase; D008856:Microscopy, Fluorescence; D009693:Nucleic Acid Hybridization; D012377:Rodentia
D015342:DNA Probes; D005456:Fluorescent Dyes