Time lapse and microscopic examinations of insonated in vitro cells.
A fibroblast cell line (Balb/c 3T3) was sham/exposed to ultrasound (2.2 MHz resonant frequency, 35.4 W/cm2 SPTP, 3 microseconds pulse duration, 200 Hz prf) for 30 min and subsequently scored for cell motility and alterations in cell morphology. Cell motility was assessed by computerized tracking of cells. Cell morphology ("normal" or "abnormal") was determined blindly by three independent scorers. A positive control (1-4 Gy) yielded statistically significant alterations in cell motility and morphology; no such differences were observed for the ultrasound regimen. The results thus fail to confirm an earlier report by Liebeskind et al. (Brit. J. Cancer 45(Suppl. V):176-186; 1982) of ultrasound-induced alterations in cell motility and morphology.
Miller
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MW
Department of Biophysics, School of Medicine and Dentistry, University of Rochester, New York 14642.
Church
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CC
Ciaravino
V
V
D000818:Animals; D002465:Cell Movement; D002999:Clone Cells; D005347:Fibroblasts / Q000502:physiology* / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D013997:Time Factors; D014465:Ultrasonics