title: Rapid technique for cryopreservation of Echinococcus multilocularis metacestodes.
abstract: Cysts of E. multilocularis were minced to prepare a crude homogenate and after addition of glycerol at a final concentration of 10%, cryopreservation was performed at a rate of 1 degree C min-1 in a controlled-rate freezer. The aliquots were subsequently stored in liquid nitrogen. All 22 isolates tested were successfully cryopreserved and their viability maintained.
authors.affiliation: Laboratoire de Parasitologie, Faculté de Médecine, Creteil, France.
authors.forename: S
authors.initials: S
authors.lastname: Bretagne
authors.forename: F
authors.initials: F
authors.lastname: Beaujean
authors.forename: A
authors.initials: A
authors.lastname: Uner
authors.forename: S
authors.initials: S
authors.lastname: Bresson-Hadni
authors.forename: M
authors.initials: M
authors.lastname: Liance
authors.forename: R
authors.initials: R
authors.lastname: Houin
mesh_terms: D000818:Animals; D015925:Cryopreservation; D004446:Echinococcus / Q000254:growth & development*