Absence of uvomorulin in a slowly compacting variant of H6 embryonal carcinoma cells.
When allowed to aggregate in calcium-containing medium, the H6 embryonal carcinoma cell variant named 6B(NG)C25 compacted more slowly than wild-type cells, and aggregates of hybrids between it and wild-type cells also compacted slowly, as if the variation (mutation) acted in a dominant fashion. In agreement with this, we now have found that the cell adhesion molecule uvomorulin is markedly reduced or absent in 6B(NG)C25 cells, as well as in the hybrids. A small amount of a higher-molecular-weight protein reacting with the antibody is present, which might represent residual uvomorulin migrating at a slower rate, an altered uvomorulin, the known precursor to uvomorulin, or an unrelated cross-reacting protein.
Littlefield
J W
JW
Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Whitehouse
L L
LL
D001345:Autoradiography; D015820:Cadherins / Q000032:analysis* / Q000378:metabolism; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D014644:Genetic Variation; D006801:Humans; D006822:Hybrid Cells / Q000032:analysis / Q000166:cytology / Q000378:metabolism; D015151:Immunoblotting; D013724:Teratoma / Q000032:analysis / Q000235:genetics / Q000473:pathology*
D015820:Cadherins