title: Nucleation of macrotubules on double-walled microtubule seeds.
abstract: It is known that histone H1 is able to cause the formation of double-walled microtubules from microtubule protein. Now, we demonstrate that in dependence on the mass ratio H1/microtubule protein upon addition of tubulin to short pieces of double-walled microtubules either their inner or their outer wall elongates resulting in normal microtubules or in macrotubules, respectively. Because of their genesis we suggest that macrotubules like double-walled microtubules (see Unger et al., Eur. J. Cell Biol. 46, 98-104 (1988)) expose those sides of tubulin dimers at their surface which usually form the lumen face of microtubules.
authors.affiliation: Central Institute of Microbiology and Experimental Therapy, Academy of Sciences of the GDR, Jena.
authors.forename: K J
authors.initials: KJ
authors.lastname: Böhm
authors.forename: W
authors.initials: W
authors.lastname: Vater
authors.forename: P
authors.initials: P
authors.lastname: Steinmetzer
authors.forename: E
authors.initials: E
authors.lastname: Unger
mesh_terms: D000818:Animals; D006657:Histones / Q000032:analysis*; D008854:Microscopy, Electron; D008868:Microtubule Proteins / Q000032:analysis*; D008870:Microtubules / Q000032:analysis / Q000648:ultrastructure*; D014404:Tubulin / Q000494:pharmacology
chemical_list: D006657:Histones; D008868:Microtubule Proteins; D014404:Tubulin