Preliminary crystallographic study of phospholipase A2 from the venom of Trimeresurus flavoviridis (habu snake).
Crystallization and a preliminary crystallographic study of Trimeresurus flavoviridis (habu snake) phospholipase A2 (PLA2) were carried out. Although crystals were obtained from various solutions, crystals suitable for X-ray analysis could be obtained from polyethylene glycol solutions only when a repeated seeding technique was applied starting from twinned crystals. The crystal is monoclinic with space group P21, with a = 44.1, b = 55.7, c = 48.8 A, and beta = 92.4 degrees. An asymmetric unit contains a dimer consisting of two identical subunits made of 122 amino acids. The crystal reflects X-rays beyond 2.5 A. A Pt derivative gave a good isomorphous crystal.
Shinoda
Y
Y
Department of Applied Chemistry, Faculty of Engineering, Nagoya University, Aichi.
Shouji
A
A
Yamane
T
T
Suzuki
A
A
Ashida
T
T
Kihara
H
H
Ohno
M
M
D000818:Animals; D003435:Crotalid Venoms; D003460:Crystallization; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D011487:Protein Conformation; D014961:X-Ray Diffraction
D003435:Crotalid Venoms; C029748:Trimeresurus venoms; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2