Ciclosporin-dependent, nu-independent, mucosal interleukin 6 response to gram-negative bacteria.
Regulation of the mucosal inflammatory response to Gram-negative bacteria was analysed. The interleukin 6 (IL-6) secretion, influx of polymorphonuclear leucocytes into urine, and bacterial clearance from the kidneys were compared between Balb/c (nu/nu) and nu/+/- mice, with and without ciclosporin (CsA) treatment. There was no significant influence of the nu genotype on any of the host responses measured. CsA pretreatment significantly decreased Il-6 secretion in both nu/nu and nu/+/- mice, but did not affect bacterial clearance or the leucocyte response in any mouse strain tested. Tissue damage, in addition to bacterial infection, resulted in significantly higher levels of IL-6 than bacterial infection alone. Tissue-damaged mice were significantly less likely to clear the bacterial infection than their non-damaged counterparts, but there was no significant difference in the leucocyte response. CsA pretreatment did not significantly reduce the levels of IL-6 in the tissue-damaged mice. These results demonstrate that the mucosal inflammatory response to Gram-negative infection, including IL-6 secretion, is nu-independent, and that bacterial infection alone or in combination with tissue damage induce IL-6 secretion by two different pathways.
Hedges
S
S
Department of Clinical Immunology, University of Göteborg, Sweden.
Linder
H
H
de Man
P
P
Svanborg Edén
C
C
D000818:Animals; D001424:Bacterial Infections / Q000276:immunology*; D003524:Cyclosporins / Q000494:pharmacology*; D006090:Gram-Negative Bacteria / Q000276:immunology; D015850:Interleukin-6 / Q000096:biosynthesis*; D007668:Kidney / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D009092:Mucous Membrane / Q000382:microbiology; D009504:Neutrophils / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D003524:Cyclosporins; D015850:Interleukin-6