title: Characterisation of Sm20, a 20-kilodalton calcium-binding protein of Schistosoma mansoni.
abstract: A cDNA clone encoding part of a 20-kDa antigen of Schistosoma mansoni (Sm20) has been isolated. The amino acid sequence of this antigen, as predicted from the sequence of the cDNA, has significant homology to the family of calcium binding proteins which include calmodulin, troponin C and the light chain of myosin. Although we have been unable to show any immunological cross-reactivity between Sm20 and calmodulins from a range of other species, we have verified that Sm20 is a functional calcium binding protein. Sm20 is encoded by a small multigene family and is expressed in schistosomula and adult worms but not in eggs. The 20-kDa nascent polypeptide appears to be post-translationally modified to give a 38-kDa species. Sm20 is present in preparations of tegumental membranes and is easily removed from intact schistosomula by detergent treatment, suggesting that it is associated with the tegument. However, the cloned portion does not appear to be exposed on the surface.
authors.affiliation: Department of Pathology, University of Cambridge, U.K.
authors.forename: J C
authors.initials: JC
authors.lastname: Havercroft
authors.forename: M C
authors.initials: MC
authors.lastname: Huggins
authors.forename: D W
authors.initials: DW
authors.lastname: Dunne
authors.forename: D W
authors.initials: DW
authors.lastname: Taylor
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002147:Calmodulin / Q000235:genetics; D003001:Cloning, Molecular; D015801:Helminth Proteins / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012550:Schistosoma mansoni / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D002135:Calcium-Binding Proteins; D002147:Calmodulin; D015801:Helminth Proteins; D008565:Membrane Proteins