title: A simple and easy-to-assemble device for polymerase chain reaction.
abstract: In this paper we describe an efficient polymerase chain reaction device which is easy to assemble and requires minimal investment in dedicated equipment. The polymerase chain reaction device consists of three waterbaths, three dual-head peristaltic pumps, an electronic timer and a fabricated water jacket capable of holding microcentrifuge tubes. This device has been successfully used to amplify human factor X genomic DNA in our laboratory.
authors.affiliation: Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284.
authors.forename: P
authors.initials: P
authors.lastname: Jagadeeswaran
authors.forename: K J
authors.initials: KJ
authors.lastname: Rao
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Zhou
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D005091:Exons; D005170:Factor X / Q000235:genetics; D005784:Gene Amplification; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000295:instrumentation*
chemical_list: D009838:Oligodeoxyribonucleotides; D005170:Factor X; D004247:DNA