Immunohistochemical demonstration of carcinogen-DNA adducts in target and non-target tissues of rats given a prostate carcinogen, 3,2'-dimethyl-4-aminobiphenyl.
An immunohistochemical procedure was applied which allows accurate localization of DNA lesions within organs and tissues of rats given 3,2'-dimethyl-4-aminobiphenyl (DMAB) using polyclonal antibodies against DMAB-DNA adducts. Dose-related nuclear staining was observed in organs regardless of DMAB-carcinogenic organotropism. In the male accessory sex organs, the lateral lobe of the prostate, a non-target site, demonstrated a similar staining intensity to that found for the ventral prostate and seminal vesicle, target sites. Orchiectomy and pretreatment with ethinyl estradiol resulted in a moderate to slight decrease in binding in the accessory sex organs. No observable decrease in staining intensity was evident in most organs 168 h after the administration of DMAB. These findings suggest that DNA adduct formation itself is not necessarily sufficient for tumor induction.
Shirai
T
T
First Department of Pathology, Nagoya City University Medical School, Japan.
Nakamura
A
A
Fukushima
S
S
Tada
M
M
Morita
T
T
Ito
N
N
D000611:Aminobiphenyl Compounds / Q000378:metabolism / Q000633:toxicity*; D000818:Animals; D002273:Carcinogens / Q000378:metabolism / Q000633:toxicity*; D004247:DNA / Q000032:analysis* / Q000187:drug effects / Q000378:metabolism; D004249:DNA Damage; D005837:Genitalia, Male / Q000032:analysis; D007150:Immunohistochemistry; D008297:Male; D009928:Organ Specificity; D010179:Pancreas / Q000032:analysis; D011467:Prostate / Q000032:analysis; D011471:Prostatic Neoplasms / Q000032:analysis / Q000139:chemically induced* / Q000473:pathology; D051381:Rats; D011916:Rats, Inbred F344; D001743:Urinary Bladder / Q000032:analysis
D000611:Aminobiphenyl Compounds; D002273:Carcinogens; C001042:2',3-dimethyl-4-aminobiphenyl; D004247:DNA