title: Insect olfactory neurons in vitro: morphological and immunocytochemical characterization of male-specific antennal receptor cells from developing antennae of male Manduca sexta.
abstract: Sex-pheromone components released by Manduca sexta females are detected solely by male-specific olfactory receptor neurons that innervate long sensilla trichodea on the male antennae. To facilitate studies of the development and physiology of these receptor cells, we have produced primary in vitro cultures of cells dissociated from pupal male antennae. These cultures comprise several morphological types of cells, 2 of which have been characterized immunocytochemically with a pair of monoclonal antibodies that were shown previously to recognize certain antigens in olfactory receptor neurons at defined stages of development. The good correlation between in vivo and in vitro expression of these antigens suggests that the immunocytochemically recognized cells are olfactory receptor neurons that follow at least partially their normal course of differentiation in vitro.
authors.affiliation: Arizona Research Laboratories, University of Arizona, Tucson 85721.
authors.forename: M
authors.initials: M
authors.lastname: Stengl
authors.forename: J G
authors.initials: JG
authors.lastname: Hildebrand
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology*; D007150:Immunohistochemistry; D007915:Lepidoptera / Q000502:physiology*; D009036:Moths / Q000254:growth & development / Q000502:physiology*; D009474:Neurons / Q000276:immunology / Q000648:ultrastructure*; D009833:Olfactory Pathways / Q000166:cytology* / Q000276:immunology; D012679:Sense Organs / Q000166:cytology* / Q000254:growth & development / Q000276:immunology; D011984:Sensory Receptor Cells / Q000276:immunology / Q000648:ultrastructure*; D012727:Sex Characteristics
chemical_list: D000911:Antibodies, Monoclonal