Latent form of transforming growth factor-beta 1 acts as a potent growth inhibitor on a human erythroleukemia cell line.
Recombinant latent form of transforming growth factor-beta 1 (L-TGF-beta 1) is activated by various chemical treatments, including acidification and heating. However, cellular mechanisms that release transforming growth factor-beta (TGF-beta) in an active form have not been fully elucidated. Investigated herein are the effects of L-TGF-beta 1 on various leukemic cell lines. Heat-activated L-TGF-beta 1 inhibited colony formation of U937, KG-1 and HL-60, whereas untreated L-TGF-beta 1 had only a marginal effect on these cells. In contrast, colony formation of human erythroleukemia cell line (HEL) was markedly inhibited by both heat-activated and untreated L-TGF-beta 1. In vitro incubation of L-TGF-beta 1 with HEL cells did not release the active form in the culture supernatants. These results suggest that HEL cells are capable of activating L-TGF-beta 1, but only in a cell-associated manner. Since HEL cells produce L-TGF-beta 1, it may act as an autocrine negative growth factor on these cells.
Piao
Y F
YF
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Ichijo
H
H
Miyagawa
K
K
Ohashi
H
H
Takaku
F
F
Miyazono
K
K
D000818:Animals; D002335:Carnivora; D002478:Cells, Cultured; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology*; D011994:Recombinant Proteins / Q000494:pharmacology; D015291:Transforming Growth Factors / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects*
D011994:Recombinant Proteins; D015291:Transforming Growth Factors