Protein chemical analysis of purified murine lamin B identifies two distinct polypeptides B1 and B2.
Lamin B purified from murine EAT cells was characterized by partial protein sequences. Contrary to the current view that mammals express only a single lamin B polypeptide corresponding to a characterized murine cDNA clone, our analysis documents two distinct B lamins. One protein follows the established cDNA sequence while the other identifies a novel murine lamin B. Comparison with the two chicken lamin B sequences established by cDNA cloning identifies the first murine lamin B sequence as a B1 type and the second as a B2 type. We conclude that mammals express two distinct lamin B forms as established by others for chicken.
Weber
K
K
Max Planck Institute for Biophysical Chemistry, Department of Biochemistry, Goettingen, FRG.
Plessmann
U
U
Traub
P
P
D000595:Amino Acid Sequence; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis; D002645:Chickens; D003488:Cyanogen Bromide; D034921:Lamin Type B; D034882:Lamins; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000032:analysis / Q000302:isolation & purification*; D010446:Peptide Fragments; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured
D034921:Lamin Type B; D034882:Lamins; D009687:Nuclear Proteins; D010446:Peptide Fragments; D003488:Cyanogen Bromide