[Involvement of tumor necrosis factor in the generation of LAK killer cells].
The effect of Tumor Necrosis Factor (TNF) alpha on LAK generation was investigated. TNF was found to act synergistically with low concentrations of IL-2 (15 pM), which were ineffective by themselves to promote the differentiation of Large granular lymphocytes (LGL) into LAK effectors. The IL-2/TNF driven generation of LAK activity involves the induction of high affinity IL-2 receptors on LGL and occurs without promoting a significant proliferation, suggesting a functional activation rather than a proliferative expansion of LAK precursors. Our studies also indicate the existence of a correlation between the generation of LAK activity and the increase in TNF binding sites on LGL. Furthermore we demonstrate that the failure of low doses of IL-2 to induce LAK activity were related to their incapacity to induce TNF production by LGL and suggest that TNF alpha may be a physiologic mediator in the sequential activation stages of LGL into LAK effectors.
Chouaib
S
S
Laboratoire d'Immunologie, Institut Gustave-Roussy, Ville-Juie, France.
Blay
J Y
JY
D002454:Cell Differentiation / Q000187:drug effects; D004357:Drug Synergism; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D015979:Killer Cells, Lymphokine-Activated / Q000166:cytology*; D011955:Receptors, Drug / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D007376:Interleukin-2; D011955:Receptors, Drug; D014409:Tumor Necrosis Factor-alpha
Implications du Tumor Necrosis Factor dans la génération des cellules tueuses LAK.