Phorbol 12-myristate 13-acetate (PMA) increases the expression of the nerve growth factor (NGF) gene in mouse L-929 fibroblasts.
The rise of the NGF mRNA pool which takes place following exposure of L-929 fibroblasts to serum was prevented in the presence of 5 microM K-252a, a compound which inhibits several species of protein kinase activities. To characterize further this phenomenon, L-929 cells growing in a serum-free medium were exposed to cyclic nucleotide analogs, to a divalent cation ionophore or to the phorbol ester PMA. Only this latter compound induced an enhancement of the NGF mRNA pool, suggesting an involvement of protein kinase C in the upregulation of the NGF transcripts. The effects of PMA or serum also require a synthesis of protein since the level of NGF transcripts remained stable in the presence of cycloheximide.
Wion
D
D
INSERM U 298, Centre Hospitalier Régional et Universitaire, Angers, France.
Mac Grogan
D
D
Houlgatte
R
R
Brachet
P
P
D000818:Animals; D001790:Blood Physiological Phenomena; D002227:Carbazoles / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D026121:Indole Alkaloids; D051379:Mice; D009414:Nerve Growth Factors / Q000235:genetics*; D014176:Protein Biosynthesis; D011493:Protein Kinase C / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D002227:Carbazoles; D026121:Indole Alkaloids; D009414:Nerve Growth Factors; D012333:RNA, Messenger; C049985:staurosporine aglycone; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate