The effect of amperozide on uptake and release of [3H]-dopamine in vitro from perfused rat striatal and limbic brain areas.
Amperozide, a putatively antipsychotic drug, was studied for its effects on uptake and release of [3H]-dopamine in rat brain in vitro. Amperozide inhibited uptake of [3H]-dopamine in striatal chopped tissue in vitro with an IC50 of 18 microM. It also increased basal release of [3H]-dopamine from perfused rat striatal and limbic tissue in vitro at concentrations above 5 microM. Release of [3H]-dopamine from perfused rat striatal and limbic tissue stimulated with 5 microM amphetamine, was inhibited by 1 microM amperozide to 46%. No significant difference was found for the effect of amperozide on in vitro release of [3H]-dopamine from corpus striatum compared to tissue from limbic brain regions; neither on basal release nor an amphetamine-stimulated release of dopamine.
Eriksson
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Department of CNS-Research, Pharmacia LEO Therapeutics AB, Malmö, Sweden.
Christensson
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D000661:Amphetamine / Q000494:pharmacology; D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D008032:Limbic System / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009627:Nomifensine / Q000494:pharmacology; D010879:Piperazines / Q000494:pharmacology*; D011619:Psychotropic Drugs / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D010879:Piperazines; D011619:Psychotropic Drugs; C049058:amperozide; D009627:Nomifensine; D000661:Amphetamine; D004298:Dopamine