title: Primary and secondary structural analyses of glutathione S-transferase pi from human placenta.
abstract: The primary structure of glutathione S-transferase (GST) pi from a single human placenta was determined. The structure was established by chemical characterization of tryptic and cyanogen bromide peptides as well as automated sequence analysis of the intact enzyme. The structural analysis indicated that the protein is comprised of 209 amino acid residues and gave no evidence of post-translational modifications. The amino acid sequence differed from that of the deduced amino acid sequence determined by nucleotide sequence analysis of a cDNA clone (Kano, T., Sakai, M., and Muramatsu, M., 1987, Cancer Res. 47, 5626-5630) at position 104 which contained both valine and isoleucine whereas the deduced sequence from nucleotide sequence analysis identified only isoleucine at this position. These results demonstrated that in the one individual placenta studied at least two GST pi genes are coexpressed, probably as a result of allelomorphism. Computer assisted consensus sequence evaluation identified a hydrophobic region in GST pi (residues 155-181) that was predicted to be either a buried transmembrane helical region or a signal sequence region. The significance of this hydrophobic region was interpreted in relation to the mode of action of the enzyme especially in regard to the potential involvement of a histidine in the active site mechanism. A comparison of the chemical similarity of five known human GST complete enzyme structures, one of pi, one of mu, two of alpha, and one microsomal, gave evidence that all five enzymes have evolved by a divergent evolutionary process after gene duplication, with the microsomal enzyme representing the most divergent form.
authors.affiliation: Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77550.
authors.forename: H
authors.initials: H
authors.lastname: Ahmad
authors.forename: D E
authors.initials: DE
authors.lastname: Wilson
authors.forename: R R
authors.initials: RR
authors.lastname: Fritz
authors.forename: S V
authors.initials: SV
authors.lastname: Singh
authors.forename: R D
authors.initials: RD
authors.lastname: Medh
authors.forename: G T
authors.initials: GT
authors.lastname: Nagle
authors.forename: Y C
authors.initials: YC
authors.lastname: Awasthi
authors.forename: A
authors.initials: A
authors.lastname: Kurosky
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D005075:Biological Evolution; D003488:Cyanogen Bromide; D001330:Electronic Data Processing; D005982:Glutathione Transferase / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D007527:Isoenzymes; D008969:Molecular Sequence Data; D010920:Placenta / Q000201:enzymology*; D011487:Protein Conformation; D014357:Trypsin
chemical_list: D007527:Isoenzymes; D005982:Glutathione Transferase; D014357:Trypsin; D003488:Cyanogen Bromide