title: Production of monoclonal antibody against a hemolysin (Vp-TRH) produced by Vibrio parahaemolyticus.
abstract: The antigenicity of a hemolysin (Vp-TRH: Vp-TDH related hemolysin) produced by Kanagawa phenomenon-negative clinical isolates of Vibrio parahaemolyticus was studied using monoclonal antibodies (MAbs). A total of 12 hybridoma clones which produced MAbs against Vp-TRH were established. All MAbs contained the Kappa light chain and were IgG type. These MAbs were divided into a minimum of 5 different specificity groups, including antibodies specific to Vp-TRH and common to both Vp-TRH and Vp-TDH, a possible pathogenic toxin of Kanagawa phenomenon-positive V. parahaemolyticus. These results clearly show the immunological similarity and dissimilarity (specificity) of Vp-TRH and Vp-TDH.
authors.affiliation: Research Institute for Microbial Diseases, Osaka University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Honda
authors.forename: Y X
authors.initials: YX
authors.lastname: Ni
authors.forename: T
authors.initials: T
authors.lastname: Miwatani
mesh_terms: D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000942:Antigens, Bacterial; D015153:Blotting, Western; D003429:Cross Reactions; D006460:Hemolysin Proteins / Q000276:immunology*; D014736:Vibrio parahaemolyticus / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D006460:Hemolysin Proteins