A facile synthesis of [14C] epicholesterol.
A facile procedure is described for the preparation of [14C]epicholesterol from [14C]cholesterol. Cholesterol is first converted to cholesteryl mesylate, which is treated with cesium acetate and 18-crown-6 in refluxing toluene to give epicholesteryl acetate. The latter is hydrolyzed, without isolation, with potassium hydroxide in tetrahydrofuran-methanol to give epicholesterol, which is obtained in pure form by preparative thin-layer chromatography.
Yan
J S
JS
Department of Chemistry and Biochemistry, Queens College, City University of New York, Flushing 11367.
Bittman
R
R
D002784:Cholesterol / Q000138:chemical synthesis*; D009682:Magnetic Resonance Spectroscopy; D008722:Methods; D008968:Molecular Conformation; D013055:Spectrophotometry, Infrared
D002784:Cholesterol