title: Mechanical properties of isolated human esophageal smooth muscle.
abstract: Isolated smooth muscle strips from the human esophagus representing both the longitudinal and circular layers of the esophagogastric junction and the esophageal body were prepared. The strips were mounted in organ baths, and resting length was defined. By repeatedly increasing the length of the strips with 20% of resting length and recording values of resting and active tensions, length-tension relations for each muscle type were constructed. Only circular strips from the esophagogastric junction developed active, resting tension, disclosed by replacing the normal Ca2(+)-containing Krebs solution with Ca2(+)-free medium. Carbachol (10(-6) M) was used for submaximal activation of the contractile apparatus. At lengths between 180 and 260% of resting length, all strips reached optimum length (LO) where further elongation gave no further increase in active tension development. Repeated stimulations with carbachol was possible at a length of 200% of LO without affecting reproducibility. Determination of different collagen components revealed no differences between muscle types.
authors.affiliation: Department of Surgical Gastroenterology L, Aarhus Municipal Hospital, Denmark.
authors.forename: A
authors.initials: A
authors.lastname: Tøttrup
authors.forename: A
authors.initials: A
authors.lastname: Forman
authors.forename: N
authors.initials: N
authors.lastname: Uldbjerg
authors.forename: P
authors.initials: P
authors.lastname: Funch-Jensen
authors.forename: K E
authors.initials: KE
authors.lastname: Andersson
mesh_terms: D002118:Calcium / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D004947:Esophagus / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D007537:Isometric Contraction / Q000187:drug effects; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology*; D013270:Stomach / Q000187:drug effects / Q000502:physiology
chemical_list: D002217:Carbachol; D002118:Calcium