Construction of an h+S strain of Schizosaccharomyces pombe.
By spontaneous in vivo integration of a mat2:1 degrees plasmid, containing a Plus (P) cassette, into an h-L MT region of Schizosaccharomyces pombe an h+ strain was obtained which neither mutates to h- nor to h90. Southern blotting showed that it possesses the same mating-type (MT) configuration as h-S except that P information resides in both cassettes. Therefore the strain was called h+S. By crossing h+S with the h- strain LK42 of Engelke et al. (1987) it was possible to obtain h- recombinants with the MT configuration mat1:1(M)smt-o-L-mat2:3(P). Because of the totally defective smt signal (smt-o) in these recombinants no MT switching occurs, so that M information is conserved in mat1:1; furthermore the cassette mat2:3(P) is not expressed like in strains with a K region. This proves that the K region does not cause the silencing of mat2:3(P).
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Institut für Genetik, Technische Universität Braunschweig, Federal Republic of Germany.
D015139:Blotting, Southern; D003433:Crosses, Genetic; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D049770:Genes, Mating Type, Fungal; D009154:Mutation; D010957:Plasmids; D011123:Polyploidy; D011995:Recombination, Genetic; D015183:Restriction Mapping; D004718:Saccharomycetales / Q000235:genetics*; D012568:Schizosaccharomyces / Q000235:genetics*