title: Interaction between rat serum phosphorylcholine binding protein and platelet activating factor.
abstract: The binding of rat serum phosphorylcholine binding protein (PCBP) to platelet activating factor (PAF) has been demonstrated using a HPLC-gel filtration technique. The bulk of the bound [3H]-PAF eluted with a higher molecular weight species of PCBP, possibly an aggregated form of PCBP. A smaller amount of [3H]-PAF co-eluted with the major monomeric species of PCBP. Formation of the PCBP-PAF complex was calcium dependent and could be inhibited by phosphorylcholine, suggesting the involvement of the phosphorylcholine binding site on PCBP. Binding of albumin and alpha 1-acid glycoprotein to PAF was not affected by phosphorylcholine or calcium. The specificity of this binding may explain the inhibitory effect of PCBP and related phosphorylcholine binding proteins on PAF induced aggregation of platelets.
authors.affiliation: Department of Biochemistry, Memorial University of Newfoundland St. John's, Canada.
authors.forename: E
authors.initials: E
authors.lastname: Randell
authors.forename: S
authors.initials: S
authors.lastname: Mookerjea
authors.forename: A
authors.initials: A
authors.lastname: Nagpurkar
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000097:blood* / Q000302:isolation & purification; D002794:Choline / Q000031:analogs & derivatives*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D010767:Phosphorylcholine / Q000097:blood*; D010972:Platelet Activating Factor / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D002352:Carrier Proteins; C030929:Crp protein, rat; D010972:Platelet Activating Factor; D010767:Phosphorylcholine; D002794:Choline