title: Pig lung 5'-nucleotidase: effect of diadenosine 5',5'''-P1, P4-tetraphosphate and its related compounds.
abstract: 1. A 5'-nucleotidase was purified from pig lung to apparent homogeneity. 2. Its kinetic properties were similar to those of the previously reported cytoplasmic 5'-nucleotidase, which preferentially hydrolyses IMP and GMP. 3. It was a tetramer composed of 69 kDa subunit. 4. It was effectively stimulated by diadenosine tetraphosphate and glycerate 2,3-bisphosphate.
authors.affiliation: National Institute of Nutrition, Tokyo, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Itoh
authors.forename: K
authors.initials: K
authors.lastname: Yamada
mesh_terms: D015720:5'-Nucleotidase / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D002850:Chromatography, Gel; D002853:Chromatography, Liquid; D015226:Dinucleoside Phosphates / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008168:Lung / Q000201:enzymology*; D008970:Molecular Weight; D013552:Swine
chemical_list: D015226:Dinucleoside Phosphates; C020733:diadenosine tetraphosphate; D015720:5'-Nucleotidase