Simultaneous measurement of tetrahydrobiopterin (THBP) and biogenic amines by liquid chromatography with electrochemical detection.
This report describes a rapid and sensitive method for measuring tetrahydrobiopterin (THBP) and biogenic amines simultaneously by liquid chromatography with electrochemical detection (LC-ECD). The coefficient of variation for THBP was 4.87% and the minimum detectable amount of THBP was approximately 20 pg. These results indicate that this simple reverse-phase ion-pair chromatography system can be used for the simultaneous analysis of endogenous THBP and biogenic amines without long sample preparation time.
Tani
Y
Y
Laboratory of Experimental Pharmacology, Suntory Institute for Biomedical Research, Osaka, Japan.
Ishihara
T
T
D000818:Animals; D001679:Biogenic Amines / Q000032:analysis*; D001708:Biopterins / Q000031:analogs & derivatives* / Q000032:analysis; D001923:Brain Chemistry; D002853:Chromatography, Liquid; D004563:Electrochemistry; D051381:Rats; D011919:Rats, Inbred Strains
D001679:Biogenic Amines; D001708:Biopterins; C003402:sapropterin