Calcium-binding proteins 33 kDa, 35 kDa, and 65/67 kDa in normal rat and Morris hepatoma tissues. A biochemical and immunohistochemical study.
Polyclonal antibodies were raised against membrane-associated calcium-binding proteins (apparent molecular masses 65000 and 67000 (CBP 65/67) and 33000 and 35000 (CBP 33 and CBP 35)), which were isolated from rat liver and Morris hepatoma. Using immunoblotting, various amounts of CBP 33 and CBP 35 as well as CBP 65/67 were detected in most rat organs. Using alkaline phosphatase and monoclonal-anti-alkaline phosphatase antibodies (APAAP), all the calcium-binding proteins were detected by immunohistochemical techniques in the plasma membranes of many cells, such as vascular endothelial cells, lymphocytes, epididymal principal cells, secretory and excretory duct cells of certain exocrine glands, straight distal tubular cells of the kidney, and in the cytoplasm of muscle cells and fibres as well as nerve cells and chondrocytes, and in connective tissue elements. Immunohistochemical analysis also showed that in polarized epithelial cells, e.g., renal tubular cells, epididymal principal cells or excretory duct cells, these calcium-binding proteins are present exclusively or mostly in the luminal plasma membrane.
Josić
D
D
Institut für Molekularbiologie und Biochemie, Freien Universität Berlin.
Gossrau
R
R
Habermann
R
R
Lim
Y P
YP
Reutter
W
W
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002135:Calcium-Binding Proteins / Q000302:isolation & purification*; D002462:Cell Membrane / Q000032:analysis; D005260:Female; D015151:Immunoblotting; D007150:Immunohistochemistry; D008099:Liver / Q000032:analysis* / Q000648:ultrastructure; D008114:Liver Neoplasms, Experimental / Q000032:analysis* / Q000648:ultrastructure; D008297:Male; D008565:Membrane Proteins / Q000032:analysis; D008970:Molecular Weight; D051381:Rats; D011915:Rats, Inbred BUF; D011916:Rats, Inbred F344; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
D000906:Antibodies; D002135:Calcium-Binding Proteins; D008565:Membrane Proteins