Comparison of fortified calf serum, serum substitutes and fetal calf serum with or without extenders for propagation of cell cultures for virus plaque assays.
Two studies comparing sewage-isolated and laboratory stock viruses were conducted to determine if alternative forms of serum or serum extenders could be used in place of fetal bovine serum without a significant loss of viral titer. In the first study, BGM cells were grown in standard MEM-L15 medium which was supplemented with Nuserum, Sigma serum replacement (CPSR-1), HyClone defined iron supplemented calf bovine serum, fetal bovine serum (FBS) or FBS supplemented with either SerXtend or Mito serum extenders. Comparison of virus titers showed that CPSR-1 gave the best overall results and was comparable to FBS. Of the serum extenders, only SerXtend improved virus recovery from sewage samples. In the second study, all sera were tested with and without SerXtend. In these experiments, SerXtend enhanced virus sensitivity of the BGM cell line grown in the HyClone serum but reduced the sensitivity of those cultured in Sigma serum. In both series, the growth of BGM cells was monitored for 12 weeks and all test products were shown to support long-term cell growth.
Dahling
D R
DR
Virology Branch, U.S. Environmental Protection Agency, Cincinnati, OH 45268.
Wright
B A
BA
D000704:Analysis of Variance; D000818:Animals; D001769:Blood; D001802:Blood Substitutes; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D010948:Viral Plaque Assay / Q000379:methods*; D014780:Viruses / Q000254:growth & development
D001802:Blood Substitutes; D003470:Culture Media