The hemoglobin of the aquatic snail, Planorbella duryi (Wetherby).
1. The hemoglobin of the pond snail, Planorbella duryi has a molecular weight of 1.64 x 10(6) to 1.77 x 10(6) as determined by light-scattering at 630 nm and a sedimentation coefficient of 36 S. 2. The analysis of the circular dichroism spectrum obtained in the 190-250 nm region suggests a high degree of helical folding of the polypeptide chains of P. duryi hemoglobin analogous to human hemoglobin and myoglobin, with estimates of alpha-helical folding of about 60-65%, 0-5% beta-structure, and the remaining portion of the chains in unordered form. 3. The dissociated subunits in 6.0 M GdmCl, in the absence and in the presence of reducing reagent (0.1 M dithiothreitol), have a molecular weight of 3.73 +/- 0.23 x 10(5) and 1.93 +/- 0.04 x 10(5), suggesting a di-decameric assembly of the parent hemoglobin organized in the form of five dimers held together by disulfide-linkages. 4. The native hemoglobin is strongly resistant to both pH dissociation and dissociation by urea and such salts as NaCl and NaClO4. Dissociation and denaturation could only be effected in concentrated GdmCl solutions. 5. The influence of the various dissociating agents on the quaternary structure suggest ionic stabilization of the decameric assembly, which is stabilized by salt bridges between the subunits.
Herskovits
T T
TT
Department of Chemistry, Fordham University, Bronx, NY 10458.
Hamilton
M G
MG
D000818:Animals; D002942:Circular Dichroism; D006454:Hemoglobins; D006863:Hydrogen-Ion Concentration; D008027:Light; D008970:Molecular Weight; D011487:Protein Conformation; D011489:Protein Denaturation; D012492:Salts; D012542:Scattering, Radiation; D012908:Snails / Q000378:metabolism*; D014508:Urea
D006454:Hemoglobins; D012492:Salts; D014508:Urea