title: Method for determining paracetamol in whole blood by chronoamperometry following enzymatic hydrolysis.
abstract: A method is proposed for the determination of paracetamol in whole undiluted blood, based on the enzymatic hydrolysis of the drug to p-aminophenol, which is then measured by chronoamperometry at a glassy carbon electrode. Hydrolysis of the paracetamol prior to electro-oxidation is shown to alleviate problems that arise from high background currents in the whole blood and so ensures a good linear correlation (r greater than 0.99) between the current and the paracetamol concentration. Recovery experiments and comparison with a reference method based on spectrophotometry suggest that the electrochemical assay only measures that proportion of paracetamol that is not bound to serum albumin.
authors.affiliation: Centre for Biotechnology, Imperial College of Science, Technology & Medicine, London, UK.
authors.forename: H
authors.initials: H
authors.lastname: Bramwell
authors.forename: A E
authors.initials: AE
authors.lastname: Cass
authors.forename: P N
authors.initials: PN
authors.lastname: Gibbs
authors.forename: M J
authors.initials: MJ
authors.lastname: Green
mesh_terms: D000082:Acetaminophen / Q000097:blood*; D000581:Amidohydrolases; D000627:Aminophenols / Q000032:analysis; D004563:Electrochemistry; D004566:Electrodes; D006801:Humans
chemical_list: D000627:Aminophenols; D000082:Acetaminophen; D000581:Amidohydrolases; C019732:aryl acylamidase; C026729:4-aminophenol