Significance and redox state of SH groups in pyruvate carrier isolated from bovine heart mitochondria.
The role and properties of -SH groups of purified pyruvate (monocarboxylate) carrier were investigated. After isolation, this protein has all -SH groups in the oxidized state. Upon reduction, the carrier can be labelled with eosin-5-maleimide. The shift in apparent Mr after the labelling points to the presence of at least two cysteine residues. Pyruvate uptake in the reconstituted system is inhibited by both permeable (eosin-5-maleimide at 1 mM concentration) and impermeable (mersalyl, p-chloromercuribenzoate) -SH group reagents. Phenylarsine oxide inhibits pyruvate transport only slightly (20%), but the inhibition is enhanced after preincubation with the substrate.
Nałecz
K A
KA
Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, Warsaw, Poland.
Müller
M
M
Zambrowicz
E B
EB
Wojtczak
L
L
Azzi
A
A
D000818:Animals; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D002417:Cattle; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008081:Liposomes; D026901:Membrane Transport Proteins; D008929:Mitochondria, Heart / Q000378:metabolism*; D027501:Monocarboxylic Acid Transporters; D010084:Oxidation-Reduction; D011773:Pyruvates / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D013439:Sulfhydryl Reagents / Q000494:pharmacology
D002352:Carrier Proteins; D008081:Liposomes; D026901:Membrane Transport Proteins; D027501:Monocarboxylic Acid Transporters; D011773:Pyruvates; D013438:Sulfhydryl Compounds; D013439:Sulfhydryl Reagents