title: The ribonuclease from an extinct bovid ruminant.
abstract: The sequence of the ribonuclease from the ancestor of swamp buffalo, river buffalo, and ox, corresponding approximately to Pachyportax latidens, an extinct ruminant known from the fossil record, has been reconstructed using the rule of 'maximum parsimony'. This protein and two sequences that may have been intermediates in the evolution of modern ribonuclease have been constructed in the laboratory by site-directed mutagenesis, and their properties examined.
authors.affiliation: Laboratory for Organic Chemistry, E.T.H., Zurich, Switzerland.
authors.forename: J
authors.initials: J
authors.lastname: Stackhouse
authors.forename: S R
authors.initials: SR
authors.lastname: Presnell
authors.forename: G M
authors.initials: GM
authors.lastname: McGeehan
authors.forename: K P
authors.initials: KP
authors.lastname: Nambiar
authors.forename: S A
authors.initials: SA
authors.lastname: Benner
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005075:Biological Evolution; D005580:Fossils; D007700:Kinetics; D008969:Molecular Sequence Data; D012260:Ribonucleases / Q000032:analysis*; D012418:Ruminants / Q000378:metabolism*
chemical_list: D012260:Ribonucleases