title: Establishment from an adult leukemic patient of two novel precursor B cell lines with different growth modality.
abstract: Two novel cell lines, PC-53 and PC-53A were established from an adult ALL patient, at third relapse and in the terminal accelerated phase respectively. Both lines displayed the phenotype of B-cell precursors (CD19+, CD38+, CD20-, cytoplasmic-mu-, immunoglobulin gene rearrangement), identical to the freshly isolated blast cells. Chromosomal analysis showed a prominent 45-XX karyotype, including three marker chromosomes. No chromosome 8 abnormalities were detectable, consistently with a non-rearranged c-myc locus. Both cell lines were EBV-negative. Growth stimulation by autologous supernatant was observed for PC-53 cells during the first 4 months in culture, whereas it was much less evident for PC-53A cells. Thus, PC-53 and PC-53A cells represent a useful tool to investigate the mechanisms involved in the clonal expansion of B-cell precursors.
authors.affiliation: Dipartimento di Medicina ed Oncologia Spermientale, Sezione di Ematologia, Torino, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Tarella
authors.forename: P
authors.initials: P
authors.lastname: Pregno
authors.forename: E
authors.initials: E
authors.lastname: Gallo
authors.forename: D
authors.initials: D
authors.lastname: Caracciolo
authors.forename: S
authors.initials: S
authors.lastname: Stefani
authors.forename: C
authors.initials: C
authors.lastname: Barletta
authors.forename: L
authors.initials: L
authors.lastname: Lanfrancone
authors.forename: D
authors.initials: D
authors.lastname: Ferrero
mesh_terms: D000328:Adult; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D015139:Blotting, Southern; D002455:Cell Division; D002460:Cell Line; D005260:Female; D006801:Humans; D007621:Karyotyping; D009857:Oncogenes / Q000235:genetics; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood* / Q000235:genetics