Colorimetric deoxyribonucleic acid hybridization assay for rapid screening of Salmonella in foods: collaborative study.
A collaborative study was performed in 11 laboratories to validate a colorimetric DNA hybridization (DNAH) method for rapid detection of Salmonella in foods. The method was compared to the standard culture method for detection of Salmonella in nonfat dry milk, milk chocolate, soy isolate, dried whole egg, ground black pepper, and raw ground turkey. Samples inoculated with high (0.4-2 cells/g) and low (0.04-0.2 cells/g) levels of Salmonella and uninoculated control samples were included in each food group analyzed. There was no significant difference in the proportion of samples positive by DNAH and culture procedure for any of the 6 foods. The colorimetric DNA hybridization assay screening method has been adopted official first action as a rapid screening method for detection of Salmonella in all foods.
Curiale
M S
MS
Silliker Laboratories, Chicago Heights, IL 60411.
Klatt
M J
MJ
Mozola
M A
MA
D000818:Animals; D002099:Cacao / Q000032:analysis; D002417:Cattle; D003124:Colorimetry; D003212:Condiments / Q000032:analysis; D004269:DNA, Bacterial / Q000032:analysis*; D004531:Eggs / Q000032:analysis; D005516:Food Microbiology; D007202:Indicators and Reagents; D008460:Meat / Q000032:analysis; D008892:Milk / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D012475:Salmonella / Q000302:isolation & purification*; D013025:Glycine max / Q000032:analysis; D014422:Turkeys
D004269:DNA, Bacterial; D007202:Indicators and Reagents