Adoptive transfer of murine autoimmune orchitis with sperm-specific T lymphoblasts.
A protocol was successfully developed for reproducibly transferring experimental autoimmune orchitis (EAO) to naive recipient mice with sperm-specific T lymphoblasts. Cell donors were Balb/c mice immunized about 12 days earlier with homologous epididymal sperm capacitated in vitro with complete Freund's adjuvant. Draining lymph node cells were collected and subjected to a second challenge with the same sperm antigen in vitro. Sperm-specific T lymphoblasts were isolated on Percoll density gradients and propagated in the presence of interleukin-2 for 3 days and then were transferred intraperitoneally to naive recipients. As few as 3 x 10(6) sperm-specific T lymphoblasts were able to transfer EAO, which began on day 7 as infiltration of lymphocytes and macrophages and on days 14 to 21 developed to degenerative changes of spermatids and exfoliation of germinal epithelium. These pathologic alterations resemble a delayed type of hypersensitivity. The results show that sensitized T lymphoblasts can mediate an antigen-specific, mononuclear cell-invasive lesion in autoimmune orchitis.
Feng
Z Y
ZY
Shanghai Second Medical University, Research and Training Center for Reproductive Medicine, People's Republic of China.
Ming
W
W
Li
L D
LD
Louis
J
J
Fei
W Y
WY
D000818:Animals; D001327:Autoimmune Diseases / Q000276:immunology*; D002455:Cell Division / Q000502:physiology; D004195:Disease Models, Animal; D007116:Immunization, Passive; D008213:Lymphocyte Activation / Q000276:immunology* / Q000502:physiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009920:Orchitis / Q000276:immunology*; D013094:Spermatozoa / Q000276:immunology*; D013737:Testis / Q000276:immunology / Q000473:pathology*