Accumulation of oleate-rich cholesteryl esters by acetyl-LDL in macrophages in the presence of an acyl-CoA: cholesterol acyltransferase inhibitor (Sandoz 58-035).
The mechanism through which cholesteryl esters rich in oleic acid accumulate in the cytoplasm was studied. The fatty acid composition of the cholesteryl esters in acetyl-LDL was high in linoleic acid, while that of cholesteryl ester inclusion bodies accumulated in the cytoplasm was high in oleic acid. This compositional change of fatty acids in cholesteryl esters occurred even in the presence of an acyl-CoA: cholesterol acyltransferase (ACAT) inhibitor, Sandoz 58-035. These results suggest that oleate-rich cholesteryl esters accumulated in the cytoplasm, even though the reesterification in microsome was inhibited by an ACAT inhibitor.
Shimasaki
O
O
Department of Microbiology and Molecular Pathology, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Mineo
C
C
Mowri
H
H
Ohkuma
S
S
Takano
T
T
D000577:Amides / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D002788:Cholesterol Esters / Q000032:analysis / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D009829:Oleic Acids / Q000378:metabolism*; D017646:Organosilicon Compounds; D002785:Sterol O-Acyltransferase / Q000037:antagonists & inhibitors*
D000577:Amides; D002788:Cholesterol Esters; D005227:Fatty Acids; D008077:Lipoproteins, LDL; D009829:Oleic Acids; D017646:Organosilicon Compounds; C032629:acetyl-LDL; C040256:SAN 58035; D002785:Sterol O-Acyltransferase