Platelet membrane defects in fawn hooded bleeder rats.
An inbred strain of fawn hooded rats with a congenital platelet defect shows a marked bleeding tendency with prolonged bleeding time. This haemorrhagic disorder has been exclusively related to a deficiency of nucleotides in platelet dense granules. When tested in cell electrophoresis platelets from fawn hooded bleeder rats showed a significantly lower electrophoretic mobility than normal rat platelets. Subsequent studies on the platelet membrane protein pattern by high resolution two-dimensional gel electrophoresis revealed the deficiency of a membrane glycoprotein (apparent molecular mass 90.000, isoelectric point 5.6), which is detectable in normal rat platelets after surface labeling by periodate-tritiated sodium borohydride. It seems likely, that this glycoprotein defect contributes at least partially to the disorder of platelet function in fawn hooded bleeder rats.
Kirchmaier
C M
CM
Center of Internal Medicine, J.W. Goethe-University, Frankfurt/Main, FRG.
Meyer
M
M
Spangenberg
P
P
Heller
R
R
Haroske
D
D
Breddin
H K
HK
Till
U
U
D000818:Animals; D001791:Blood Platelet Disorders / Q000097:blood*; D001797:Blood Protein Electrophoresis; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D010979:Platelet Function Tests; D010980:Platelet Membrane Glycoproteins / Q000032:analysis / Q000172:deficiency*; D010981:Platelet Storage Pool Deficiency / Q000097:blood*; D051381:Rats; D011919:Rats, Inbred Strains / Q000097:blood*; D011922:Rats, Mutant Strains / Q000097:blood*
D010980:Platelet Membrane Glycoproteins