A highly repeated retropseudogene-like sequence in DNA of the redbreasted merganser (Mergus serrator).
Two highly repeated nucleotide sequences (RBMI and RBMII) cloned from an EcoRI digest of DNA of the redbreasted merganser (Mergus serrator) account for approx. 5 to 10% of the DNA of M. serrator and the closely related Mergus merganser. Complete DNA digestion of seven members of the Mergini with EcoRI produces distinct, relatively species-specific patterns of a few high-Mr (greater than 1.5 kb) fragments of RBMI-like material. In such digests RBMII forms ladder-type patterns with monomers of approx. 200 bp. The sequence of a cloned 2.6-kb RBMI fragment from M. serrator contains several extended (up to 70 bp) and modified poly(dA) sequences, two open reading frames in opposite orientation to the longest poly(dA) sequence and two direct 10-bp repeats suggesting that RBMI is a rearranged retropseudogene-like element.
McHugh
K P
KP
Institute of Molecular Biophysics, Florida State University, Tallahassee 32306.
Madsen
C S
CS
de Kloet
S R
SR
D000818:Animals; D001483:Base Sequence; D001717:Birds / Q000235:genetics*; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D008969:Molecular Sequence Data; D011544:Pseudogenes; D012091:Repetitive Sequences, Nucleic Acid
D004247:DNA