Cytosolic free calcium concentrations in lymphocytes from malignant hyperthermia susceptible patients.
Halothane in concentrations exceeding 1.2 mmol litre-1 increased (P less than 0.05) the apparent intracellular concentration of calcium in lymphocytes from 12 patients being tested for susceptibility to malignant hyperthermia (MH) using the fluorescent Ca2+ indicator, fura2. There was no difference in [Ca2+]i between lymphocytes from patients found to be MH susceptible (n = 5) on in vitro contracture testing with halothane and caffeine and those from MH negative patients (n = 6). Thus determination of [Ca2+]i in lymphocytes after exposure to halothane could not be used as a diagnostic test for MH susceptibility.
Ording
H
H
Danish Malignant Hyperthermia Register, Department of Anaesthesia, Herlev Hospital.
Foder
B
B
Scharff
O
O
D000328:Adult; D000368:Aged; D001572:Benzofurans; D002118:Calcium / Q000097:blood*; D002648:Child; D003600:Cytosol / Q000378:metabolism*; D004198:Disease Susceptibility; D005260:Female; D016257:Fura-2; D006221:Halothane / Q000494:pharmacology; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008305:Malignant Hyperthermia / Q000097:blood / Q000175:diagnosis*; D008875:Middle Aged
D001572:Benzofurans; D002118:Calcium; D016257:Fura-2; D006221:Halothane