Automated isolation of mononuclear cells using the Fenwal CS3000 blood cell separator.
We describe a method for in vitro isolation of mononuclear cells from peripheral blood or bone marrow using a Fenwal CS3000 Apheresis device without employing density gradients or sedimenting agents. The automatic processing program requires minimal operator intervention and no subjective operator decisions. A mean of 67% of starting mononuclear cells were recovered in a 100 ml product having 95% mononuclear cells and less than 1% of the original red blood cells. The average processing time was 35 minutes.
Areman
E M
EM
Division of Transfusion Medicine and Bone Marrow Transplantation, Georgetown University Hospital, Washington, D.C.
Cullis
H
H
Sacher
R A
RA
Cottler-Fox
M
M
Deeg
H J
HJ
D001854:Bone Marrow Cells; D002469:Cell Separation / Q000295:instrumentation*; D006801:Humans; D007963:Leukocytes, Mononuclear