Microcalorimetric studies on metabolism of hepatic tissue. II. Measurements of ischemic tissue.
Biopsies of ischemic livers demonstrated a significantly higher rate of heat production when incubated in Leibowitz L-15 medium compared to incubation in modified Eagle's solution (P less than 0.01). A maximal reduction of 25% in the rate of heat production was observed after 60 min of induced ischemia. Liver tissue undergoing reperfusion showed complete and partial recovery after 60 and 90 min, respectively. We conclude that the viability of liver tissue begins to deteriorate after 1-1.5 h of ischemia. Using the level of adenosine triphosphate (ATP) as a parameter for viability, others had found this critical time period to be 1.5-2 h. Furthermore, we conclude that monitoring heat production of tissue provides a useful measure of viability for both clinical application and in connection with experimental liver surgery.
Asakawa
H
H
Research Department of Surgery, University Hospital, Lund, Sweden.
Nässberger
L
L
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001833:Body Temperature Regulation; D002151:Calorimetry; D002374:Catalase / Q000494:pharmacology; D007511:Ischemia / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D015424:Reperfusion; D013482:Superoxide Dismutase / Q000494:pharmacology
D000255:Adenosine Triphosphate; D002374:Catalase; D013482:Superoxide Dismutase