Extracts of hamster cells abortively infected with human adenovirus type 12 are competent to support initiation of viral DNA replication.
Baby hamster kidney (BHK-21) cells do not allow replication of human adenovirus type 12 (Ad12) DNA during abortive infection by this virus. However, we have determined that crude extracts of BHK-21 cells abortively infected with Ad12 support in vitro the initiation reaction of Ad12 DNA replication. Synthesis of the Ad12 pTP-dCMP initiation complex by BHK extracts is two- to five-fold less than when crude infected human (KB) cell extracts are used in the reaction. Combining infected KB cytoplasmic and uninfected BHK nuclear extracts in the reaction indicates that the decreased efficiency is probably due to a lesser ability of hamster nuclear extracts to support the initiation reaction, rather than to decreased synthesis of Ad12 pTP and DNA polymerase during abortive infection, or to the presence of an inhibitor in BHK cells.
Chowrira
B M
BM
Department of Biological Sciences, Illinois State University, Normal 61761.
Lucher
L A
LA
D000256:Adenoviridae / Q000502:physiology*; D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D003593:Cytoplasm / Q000382:microbiology; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D004259:DNA-Directed DNA Polymerase / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D014779:Virus Replication
D004279:DNA, Viral; D004259:DNA-Directed DNA Polymerase