title: Automated isolation of mononuclear cells using the Fenwal CS3000 blood cell separator.
abstract: We describe a method for in vitro isolation of mononuclear cells from peripheral blood or bone marrow using a Fenwal CS3000 Apheresis device without employing density gradients or sedimenting agents. The automatic processing program requires minimal operator intervention and no subjective operator decisions. A mean of 67% of starting mononuclear cells were recovered in a 100 ml product having 95% mononuclear cells and less than 1% of the original red blood cells. The average processing time was 35 minutes.
authors.affiliation: Division of Transfusion Medicine and Bone Marrow Transplantation, Georgetown University Hospital, Washington, D.C.
authors.forename: E M
authors.initials: EM
authors.lastname: Areman
authors.forename: H
authors.initials: H
authors.lastname: Cullis
authors.forename: R A
authors.initials: RA
authors.lastname: Sacher
authors.forename: M
authors.initials: M
authors.lastname: Cottler-Fox
authors.forename: H J
authors.initials: HJ
authors.lastname: Deeg
mesh_terms: D001854:Bone Marrow Cells; D002469:Cell Separation / Q000295:instrumentation*; D006801:Humans; D007963:Leukocytes, Mononuclear