Metabolic labeling of Entamoeba histolytica antigens: characterization of a 28-kDa major intracellular antigen.
The in vivo incorporation of radiolabeled amino acids into antigens of Entamoeba histolytica, HM-1:IMSS, is reported. Immunoprecipitation with sera from patients with invasive amebiasis revealed a 28-kDa antigen present in whole cell lysates of E. histolytica. This antigen was of cytoplasmic origin, as indicated by cell fractionation and Triton X-114 detergent-phase separation. Immunoprecipitation, using sera from patients with invasive amebiasis and symptomless cyst passers, revealed the 28-kDa antigen as the major antigen recognized by the sera tested. Immunoprecipitation analysis using radiolabeled-released proteins instead of whole cell lysates showed a number of bands, including the 28-kDa antigen. The data suggest that the 28-kDa antigen is of cytoplasmic origin or is released from the cytoplasmic compartment.
Bhattacharya
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Laboratory of Parasitic Diseases, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Bhattacharya
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Sharma
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Diamond
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D000562:Amebiasis / Q000276:immunology*; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D003593:Cytoplasm / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004748:Entamoeba histolytica / Q000276:immunology* / Q000378:metabolism; D004749:Entamoebiasis / Q000276:immunology*; D006801:Humans; D007106:Immune Sera; D008101:Liver Abscess, Amebic / Q000276:immunology; D008970:Molecular Weight; D011233:Precipitin Tests; D015800:Protozoan Proteins / Q000378:metabolism
D000596:Amino Acids; D000953:Antigens, Protozoan; D007106:Immune Sera; D015800:Protozoan Proteins