title: Comparative analysis of collagens solubilized from human foetal, and normal and osteoarthritic adult articular cartilage, with emphasis on type VI collagen.
abstract: The different collagen types were extracted sequentially, by 4 M guanidinium chloride and pepsin, from human foetal and normal and osteoarthritic adult articular cartilage. They were characterized by electrophoresis and immunoblotting. Most of the collagenous proteins present in articular cartilage from young human foetuses were solubilized: almost 40% of the total collagen was extracted in the native form with 4 M guanidinium chloride. Type VI collagen was detected in this fraction as high-molecular-mass chains (185-220 kDa) and a low-molecular-mass chain (140 kDa). Type II, IX and XI collagens were also present, but were extracted more extensively by pepsin digestion. Comparative analysis of normal and osteoarthritic cartilage from adults reveals some major differences: an increase in the solubility of the collagen and modifications of soluble collagen types in osteoarthritic cartilage. Furthermore, type VI collagen was present at a higher concentration in guanidinium chloride extracts of osteoarthritic cartilage than those of normal tissue. This finding was corroborated by electron microscopic observations of the same samples: abundant (100 nm) periodic fibrils were observed in the disorganized pericellular capsule of cloned cells in osteoarthritic cartilage. In normal tissues the pericellular zone was more compact and contained only a few such banded fibrils. The differences in the collagen types solubilized from normal and osteoarthritic cartilage, although corresponding to a minor proportion of the total collagen, demonstrate that important modifications in chondrocyte metabolism and in the collagenous network do occur in degenerated cartilage.
authors.affiliation: Laboratoire d'Histologie Expérimentale, UA CNRS 244, Université Claude Bernard, Villeurbanne, France.
authors.forename: M C
authors.initials: MC
authors.lastname: Ronzière
authors.forename: S
authors.initials: S
authors.lastname: Ricard-Blum
authors.forename: J
authors.initials: J
authors.lastname: Tiollier
authors.forename: D J
authors.initials: DJ
authors.lastname: Hartmann
authors.forename: R
authors.initials: R
authors.lastname: Garrone
authors.forename: D
authors.initials: D
authors.lastname: Herbage
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002358:Cartilage, Articular / Q000032:analysis* / Q000196:embryology / Q000648:ultrastructure; D003094:Collagen / Q000302:isolation & purification; D006801:Humans; D015151:Immunoblotting; D008875:Middle Aged; D010003:Osteoarthritis / Q000378:metabolism*; D012995:Solubility
chemical_list: D003094:Collagen