title: Ion channels in the nuclear envelope.
abstract: Cell nuclei are capable of partitioning a wide variety of molecules from the cytosol, including macromolecules such as proteins and RNA, and smaller peptides, amino acids, sugars and Na+ and K+ ions, all of which can be accumulated in or excluded from the nuclear domain. There are two mechanisms behind this compartmentalization: selective retention of freely diffusible molecules, and selective entry through the nuclear envelope. It is generally accepted that the nuclear envelope restricts only the larger molecules. Here we apply the patch-clamp technique to isolated murine pronuclei and show that the nuclear envelope contains K(+)-selective channels which have multiple conductance states, the maximal conductance being 200 pS. These channels, which contribute to the nuclear membrane potential, may be important in balancing the charge carried by the movement of macromolecules in and out of the nucleus.
authors.affiliation: Department of Anatomy and Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322.
authors.forename: M
authors.initials: M
authors.lastname: Mazzanti
authors.forename: L J
authors.initials: LJ
authors.lastname: DeFelice
authors.forename: J
authors.initials: J
authors.lastname: Cohn
authors.forename: H
authors.initials: H
authors.lastname: Malter
mesh_terms: D000818:Animals; D004553:Electric Conductivity; D005260:Female; D008564:Membrane Potentials; D051379:Mice; D009685:Nuclear Envelope / Q000502:physiology* / Q000648:ultrastructure; D015221:Potassium Channels / Q000502:physiology*; D012519:Sarcoplasmic Reticulum / Q000502:physiology; D015053:Zygote / Q000648:ultrastructure*
chemical_list: D015221:Potassium Channels