Prostaglandin-E2 released by pre-implantation human conceptuses.
This work investigates the production of prostaglandin E2 (PGE2), a well known modulator for the suppression of immune cells, by pre-implantation human conceptuses from the 4-cell stage to the hatched blastocyst and by cumulus oophorus cells, these being obtained from an IVF/ET program. Cumulus cell complexes cultured for 48 h produced considerable amounts of PGE2, necessitating complete removal of their influence on the conceptus cultures. All stages of the human conceptus studied produced PGE2 during 48-h cultures, the greatest amounts from late blastocysts, and from those cultured in media containing bovine serum albumin rather than human donor serum. Indomethacin-treated control blastocysts confirmed the synthesis and release from the human conceptuses. The production of PGF2a from human blastocysts could not be demonstrated.
Holmes
P V
PV
Department of Obstetrics and Gynecology, University of Göteborg, Sweden.
Sjögren
A
A
Hamberger
L
L
D001755:Blastocyst / Q000378:metabolism*; D002478:Cells, Cultured / Q000378:metabolism; D015232:Dinoprostone / Q000096:biosynthesis*; D010064:Embryo Implantation; D005260:Female; D006801:Humans; D066298:In Vitro Techniques
D015232:Dinoprostone