title: Aldehyde-oxidizing enzymes in an adult moth: in vitro study of aldehyde metabolism in Heliothis virescens.
abstract: The conversion of pheromonal aldehydes to carboxylic acids in vitro in tissue extracts of Heliothis virescens is catalyzed by both aldehyde dehydrogenase and aldehyde oxidase enzymes. The aldehyde-oxidizing activity in antennae, heads, legs, and hemolymph from male and female moths was examined by radiochromatographic and spectroscopic assays. First, the enzymatic activity was measured in the presence or absence of added NAD+ using either (Z)-9-tetradecenal or (Z)-11-hexadecenal as tritiated substrate. Second, substrate specificity was determined spectroscopically by (i) indirect measurement of the AO-released hydrogen peroxide through the coupled AO-horseradish peroxidase reaction and by (ii) direct measurement of the ALDH-produced NADH. Both aldehyde-oxidizing activities were associated with soluble enzymes in the antennal extracts, and these enzymes degraded pheromone and nonpheromonal aldehydes. Both AO and ALDH activities were present in male and female tissues. AO activity was exhibited primarily in the antennal extracts and to a lesser degree in the leg extracts. Moreover, ALDH activity was distributed in the antenna, head, and leg extracts. A vinyl ketone analog of (Z)-11-hexadecenal preferentially inhibited the ALDH activity over the AO activity.
authors.affiliation: Department of Chemistry, State University of New York, Stony Brook 11794-3400.
authors.forename: M L
authors.initials: ML
authors.lastname: Tasayco
authors.forename: G D
authors.initials: GD
authors.lastname: Prestwich
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000378:metabolism*; D042931:Aldehyde Oxidase; D000445:Aldehyde Oxidoreductases / Q000378:metabolism*; D000447:Aldehydes / Q000378:metabolism*; D000466:Alkadienes / Q000494:pharmacology; D000818:Animals; D002855:Chromatography, Thin Layer; D005260:Female; D066298:In Vitro Techniques; D007915:Lepidoptera / Q000201:enzymology*; D008297:Male; D008954:Models, Biological; D009036:Moths / Q000201:enzymology* / Q000378:metabolism; D009243:NAD / Q000494:pharmacology; D010675:Pheromones / Q000378:metabolism; D012737:Sex Factors; D013379:Substrate Specificity / Q000187:drug effects
chemical_list: D000447:Aldehydes; D000466:Alkadienes; D010675:Pheromones; D009243:NAD; C063060:1,11-hexadecadien-3-one; D000445:Aldehyde Oxidoreductases; D000444:Aldehyde Dehydrogenase; D042931:Aldehyde Oxidase