An unusual structure of a putative T cell oncogene which allows production of similar proteins from distinct mRNAs.
We previously identified a putative T cell oncogene on chromosome 11 near a translocation t(11;14)(p15;q11) in a human T cell tumour. The gene is transcribed from distinct promoters which have unrelated sequences, which occur within close but distinct methylation-free islands and which allow cell specific production of mRNA. The alternative first exons each contain a protein initiation codon from which two species of protein can be made, differing by only a single amino acid. The protein sequence is highly conserved between man and mouse (98%) and the same single codon difference between alternative first exons is also conserved. This is, therefore, a new form of eukaryotic gene organization from which similar proteins can be made from distinct mRNA species.
Boehm
T
T
Medical Research Council Laboratory of Molecular Biology, Cambridge, UK.
Greenberg
J M
JM
Buluwela
L
L
Lavenir
I
I
Forster
A
A
Rabbitts
T H
TH
D000595:Amino Acid Sequence; D001483:Base Sequence; D002880:Chromosomes, Human, Pair 11; D002883:Chromosomes, Human, Pair 14; D003062:Codon / Q000235:genetics; D005091:Exons; D015870:Gene Expression; D015698:Genomic Library; D006801:Humans; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D015345:Oligonucleotide Probes; D009857:Oncogenes; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics*; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014162:Transfection; D014178:Translocation, Genetic
D003062:Codon; D009363:Neoplasm Proteins; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D011948:Receptors, Antigen, T-Cell