title: Fatty acylation promotes fusion of transport vesicles with Golgi cisternae.
abstract: Two different methods, stimulation of transport by fatty acyl-coenzyme A (CoA) and inhibition of transport by a nonhydrolyzable analogue of palmitoyl-CoA, reveal that fatty acylation is required to promote fusion of transport vesicles with Golgi cisternae. Specifically, fatty acyl-CoA is needed after the attachment of coated vesicles and subsequent uncoating of the vesicles, and after the binding of the NEM-sensitive fusion protein (NSF) to the membranes, but before the actual fusion event. We therefore suggest that an acylated transport component participates, directly or indirectly, in membrane fusion.
authors.affiliation: Department of Biology, Princeton University, New Jersey 08544-1014.
authors.forename: N
authors.initials: N
authors.lastname: Pfanner
authors.forename: B S
authors.initials: BS
authors.lastname: Glick
authors.forename: S R
authors.initials: SR
authors.lastname: Arden
authors.forename: J E
authors.initials: JE
authors.lastname: Rothman
mesh_terms: D000214:Acyl Coenzyme A / Q000494:pharmacology*; D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism*; D002474:Cell-Free System; D003065:Coenzyme A / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology; D006056:Golgi Apparatus / Q000187:drug effects / Q000378:metabolism*; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008561:Membrane Fusion / Q000187:drug effects*; D052066:N-Ethylmaleimide-Sensitive Proteins; D017830:Octoxynol; D015388:Organelles / Q000187:drug effects / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000494:pharmacology; D033921:Vesicular Transport Proteins
chemical_list: D000214:Acyl Coenzyme A; D002352:Carrier Proteins; D003902:Detergents; D033921:Vesicular Transport Proteins; D000431:Ethanol; D011092:Polyethylene Glycols; C032421:heptadecan-2-onyl-dethio-coenzyme A; D017830:Octoxynol; D052066:N-Ethylmaleimide-Sensitive Proteins; D003065:Coenzyme A