Chronic cerebral intracellular alkalosis following forebrain ischemic insult in rats.
We measured cerebral intracellular pH using in vivo phosphorus-31 nuclear magnetic resonance spectroscopy during 1 week after forebrain ischemia or sham operation in eight and seven rats, respectively. Mean maximum pH was significantly higher (p less than 0.003) in the ischemic group than in the sham-operated group (7.34 +/- 0.03 and 7.19 +/- 0.02, respectively). The difference between mean maximum pH and baseline pH (7.08 +/- 0.01 in each group) was significantly greater (p less than 0.02) in the ischemic group than in the sham-operated group. In the ischemic group, alkalosis occurred primarily after 48-72 hours of recirculation. We speculate that brain tissue alkalosis occurring chronically after ischemia is associated with delayed ischemic neuronal death.
Chopp
M
M
Center for Stroke Research and NMR, Department of Neurology, Henry Ford Hospital, Detroit, Michigan.
Vande Linde
A M
AM
Chen
H
H
Knight
R
R
Helpern
J A
JA
Welch
K M
KM
D000471:Alkalosis / Q000209:etiology* / Q000378:metabolism; D000818:Animals; D001927:Brain Diseases / Q000209:etiology* / Q000378:metabolism; D002545:Brain Ischemia / Q000150:complications*; D002908:Chronic Disease; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains