Radioresistance of rat glioma cell lines cultured as multicellular spheroids. Correlation with electrical cell-to-cell-coupling.
Two chemically induced rat glioblastomas, RG2 and F98, were cultured as monolayers and as multicellular spheroids and subjected to Co-gamma-irradiation. In parallel, intercellular communication between cells was determined as electrical coupling between neighbouring cells using micro-electrode techniques. A third glioblastoma with known radiobiological response (9L) was assayed with respect to intercellular communication and included into this analysis. Electrical coupling was low for RG2, intermediate for F98, and high for 9L. Radioresistance of spheroids, as expressed in terms of the mean inactivation dose computed from the survival curves increased in the same direction (RG2: 2.4 Gy; F98: 5.1 Gy; 9L: 6.5 Gy). A comparison of these parameters demonstrates a correlation between solid tumor radioresistance and gap-junctional cell-to-cell communication, at least for the class of glioblastomas analysed in this study.
Knedlitschek
G
G
Kernforschungszentrum Karlsruhe, Hauptabteilung Sicherheit/Biophysik.
Anderer
U
U
Weibezahn
K F
KF
Dertinger
H
H
D000818:Animals; D002450:Cell Communication / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D005038:Ethylnitrosourea; D005910:Glioma / Q000139:chemically induced / Q000532:radiotherapy*; D007365:Intercellular Junctions / Q000528:radiation effects; D008839:Microelectrodes; D011879:Radiotherapy Dosage; D051381:Rats; D014407:Tumor Cells, Cultured
D005038:Ethylnitrosourea