Higher transfection efficiency of genomic DNA purified with a guanidinium thiocyanate-based procedure.
Efficient transfection of eukaryotic cells is dependent on the purity of the transfected genomic DNA. In an attempt to obtain a more reliable method of DNA purification we have modified the widely used protocol of Blin and Stafford to include a treatment with guanidinium thiocyanate. The DNA obtained following the present protocol transfects eukaryotic cells with higher efficiency.
Alberti
S
S
Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
Fornaro
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M
D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D005796:Genes; D006146:Guanidines; D006801:Humans; D007202:Indicators and Reagents; D017879:Isothiocyanates; D007739:L Cells / Q000032:analysis; D007962:Leukocytes / Q000032:analysis; D008297:Male; D051379:Mice; D013094:Spermatozoa / Q000032:analysis; D013861:Thiocyanates; D014162:Transfection
D006146:Guanidines; D007202:Indicators and Reagents; D017879:Isothiocyanates; D013861:Thiocyanates; C054435:guanidine isothiocyanate; D004247:DNA