Inhibition by serum components of the expression of lipoprotein lipase gene upon stimulation by growth hormone.
Growth hormone regulates in a positive way the expression of the lipoprotein lipase gene at a transcriptional level in preadipocyte Ob1771 cells. Inhibition by serum components of this expression was investigated upon stimulation by growth hormone. Low-molecular weight, lipid-soluble components (a serum lipid extract, corticosteroids and oleic acid) and high-molecular weight, hydrophilic components (TGF-beta and those present in delipidated serum) were inhibitory. Inhibition of the expression of LPL mRNAs and that of LPL activity were parallel. It is concluded that the regulation of the expression of LPL gene occurs likely at a transcriptional level and that a balance between multiple effectors present in serum are active in an opposite manner.
Pradines-Figueres
A
A
Centre de Biochimie du CNRS, UPR-7300, Faculté des Sciences, Université de Nice-Sophia Antipolis, France.
Barcellini-Couget
S
S
Dani
C
C
Baudoin
C
C
Ailhaud
G
G
D000818:Animals; D001769:Blood; D002417:Cattle; D002460:Cell Line; D003345:Corticosterone / Q000494:pharmacology; D003470:Culture Media; D003907:Dexamethasone / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D013006:Growth Hormone / Q000494:pharmacology*; D007700:Kinetics; D008055:Lipids / Q000097:blood* / Q000494:pharmacology; D008071:Lipoprotein Lipase / Q000235:genetics*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects*
D003470:Culture Media; D008055:Lipids; D012333:RNA, Messenger; D003907:Dexamethasone; D013006:Growth Hormone; D008071:Lipoprotein Lipase; D003345:Corticosterone