Urinary excretion of dipeptidyl aminopeptidase i.v. in patients with renal diseases.
Enzymuria is a frequent finding in patients suffering from various kidney diseases. The present study was undertaken to evaluate the clinical value of the determination of tubule-brush-border-associated dipeptidyl aminopeptidase IV (DAP IV) in the urine of patients with acute and chronic tubulointerstitial nephritis (n = 12), chronic glomerulonephritis (n = 15), essential arterial hypertension (n = 30), after kidney transplantation (n = 20), and of healthy control persons (n = 68). DAP IV was measured in spontaneously voided mid-stream morning urine ("second morning urine"), and was expressed as enzyme activity in units/liter. In order to account for variations due to urine concentration without collecting 24-hour specimens, a urinary DAP IV/creatinine ratio (DCR) was calculated. Furthermore, patterns of proteinuria were assayed by SDS-polyacrylamide gel electrophoresis. Urinary DAP IV activity of healthy controls was 4.94 +/- 0.12 U/l (DCR: 0.46 +/- 0.30 U/mmol creatinine) with only small day to day variations. Urinary DAP IV activity in patients with tubulointerstitial nephritis was significantly higher (15.5 +/- 15.6 U/l, p less than 0.05 vs controls; DCR: 1.67 +/- 0.97 U/mmol creatinine, p less than 0.001 vs controls). In patients with chronic glomerulonephritis urinary DAP IV activity was 9.6 +/- 5.6 U/l, p less than 0.05 (DCR: 1.22 +/- 0.75 U/mmol creatinine, p less than 0.05 vs controls). Increased urinary DAP IV activity in patients with chronic glomerulonephritis was associated with a mixed glomerulo-tubular pattern of proteinuria (as determined by SDS-PAGE).(ABSTRACT TRUNCATED AT 250 WORDS)
Wolf
G
G
Department of Nephrology, University Hospital, Frankfurt/Main, FRG.
Scherberich
J E
JE
Nowack
A
A
Stein
O
O
Schoeppe
W
W
D000293:Adolescent; D000328:Adult; D003404:Creatinine / Q000097:blood / Q000652:urine; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000652:urine*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005921:Glomerulonephritis / Q000201:enzymology; D006801:Humans; D006973:Hypertension / Q000201:enzymology; D007674:Kidney Diseases / Q000201:enzymology* / Q000503:physiopathology / Q000652:urine; D016030:Kidney Transplantation; D007687:Kidney Tubules, Proximal / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D009395:Nephritis, Interstitial / Q000201:enzymology; D011507:Proteinuria / Q000150:complications
D003404:Creatinine; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases