A dual streaming potential device used as an affinity sensor for monitoring hybridoma cell cultivations.
A dual streaming potential device was used for determining the content of monoclonal antibodies in cultivation medium for hybridoma cells. Samples of culture medium were analyzed as discrete pulses, as a continuous flow of constant concentration as well as with fluctuating concentrations. Tests were done with two subclasses of IgG as well as with IgM. Finally, the analytical device was applied to the registration of production of monoclonal antibodies in a cultivation.
Miyabayashi
A
A
Department of Biotechnology, Chemical Center, University of Lund, Sweden.
Mattiasson
B
B
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D001709:Biotechnology / Q000295:instrumentation*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006825:Hybridomas; D007074:Immunoglobulin G / Q000032:analysis; D051379:Mice; D011817:Rabbits
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G