Specific localization of lectins in boar and bull spermatozoa.
The roles that complex carbohydrates play in gamete recognition and fertilization are well-documented. This study was designed to identify and map sites of N-linked oligosaccharides in boar and bull sperm. Plant lectins, conjugated with FITC, RITC and gold particles, were used as probes for specific sugar moieties in sperm membranes. Results showed strong labeling of sperm heads by most lectins. However, lectins with specificities for mannosyl, glucosyl, and sialic acid were predominantly distributed over the sperm acrosomal region. Lectins specific for fucosyl residues predominantly stained the postacrosomal regions of both boar and bull sperm. Lectin gold studies revealed that mannosyl and galactosyl-linked residues occupied the acrosomal membrane and plasma membrane in the postacrosomal region, but not the nucleus or nuclear membrane. The cytoplasmic droplet, middle piece and flagellum of boar and bull sperm were moderately stained by lectins. The microquantitative analysis confirmed the fluorescence data. The presence of fucosyl residues predominantly in the postacrosomal regions lends credence to the suggestion that glycoproteins rich in fucosyl-like residues may be a universal carbohydrate moiety involved in recognition processes in mammalian fertilization.
Ahluwalia
B
B
Department of Obstetrics and Gynecology, Howard University, Washington, D.C.
Farshori
P
P
Jamuar
M
M
Baccetti
B
B
Anderson
W A
WA
D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D006651:Histocytochemistry; D007425:Intracellular Membranes / Q000378:metabolism / Q000648:ultrastructure; D037102:Lectins / Q000378:metabolism*; D008297:Male; D008854:Microscopy, Electron; D013077:Sperm Head / Q000378:metabolism / Q000648:ultrastructure; D013094:Spermatozoa / Q000378:metabolism* / Q000648:ultrastructure; D013552:Swine
D037102:Lectins