title: Glucose is required to maintain ATP/ADP ratio of isolated bovine cerebral microvessels.
abstract: Isolated bovine cerebral microvessels (ICMV) were incubated with different metabolic fuels to determine the effect of each of them on microvessel energy state. With no fuel added to the medium, the ATP/ADP generally decreased from initial values of 1.5-3 down to 1-1.5 over 4 h; the ATP content also declined approximately 50%. In contrast, with glucose present, the ATP/ADP increased, and the ATP content was maintained. Pyruvate, beta-hydroxybutyrate, glutamate, and oleate were ineffective; oleate added together with carnitine gave some improvement but less than with glucose. Oxygen consumption by ICMV did not differ appreciably in fuel-free or glucose-containing medium. Addition of an inhibitor of fatty acid oxidation, 2-tetradecylglycidate, depressed the ATP/ADP. These results suggest that ICMV require glycolysis to maintain both their content of ATP and their ATP/ADP. They also suggest that endogenous lipid is an important fuel for isolated microvessels.
authors.affiliation: Evans Department of Medicine, Boston University School of Medicine, Massachusetts 02118.
authors.forename: C G
authors.initials: CG
authors.lastname: Gaposchkin
authors.forename: K
authors.initials: K
authors.lastname: Tornheim
authors.forename: I
authors.initials: I
authors.lastname: Sussman
authors.forename: N B
authors.initials: NB
authors.lastname: Ruderman
authors.forename: A L
authors.initials: AL
authors.lastname: McCall
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002196:Capillaries / Q000187:drug effects / Q000378:metabolism; D002331:Carnitine / Q000494:pharmacology; D002417:Cattle; D002560:Cerebrovascular Circulation / Q000187:drug effects; D004734:Energy Metabolism; D005947:Glucose / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology; D011773:Pyruvates / Q000378:metabolism
chemical_list: D009829:Oleic Acids; D011773:Pyruvates; D019301:Oleic Acid; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D005947:Glucose; D002331:Carnitine