Proton relaxation enhancement of albumin, immunoglobulin G, and fibrinogen labeled with Gd-DTPA.
Bovine serum albumin, immunoglobulin G, and fibrinogen were labeled with Gd-DTPA using a bifunctional chelating agent DTPA anhydride. The protein-(Gd-DTPA) conjugates had 1.4- to 2.0-fold greater longitudinal relaxivities at 0.02 and 0.44 T than the relaxivity of plain Gd-DTPA. The increase of longitudinal relaxivity was not directly related to the size of carrier protein. Up to 50 Gd-DTPA chelates per protein, longitudinal relaxivity of the conjugates was proportional to the concentration of Gd and independent of the Gd/protein ratio.
Paajanen
H
H
Department of Diagnostic Radiology, University of Turku, Finland.
Reisto
T
T
Hemmilä
I
I
Komu
M
M
Niemi
P
P
Kormano
M
M
D000818:Animals; D002417:Cattle; D005340:Fibrinogen; D019786:Gadolinium DTPA; D007074:Immunoglobulin G; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009942:Organometallic Compounds; D004369:Pentetic Acid; D012710:Serum Albumin, Bovine
D007074:Immunoglobulin G; D009942:Organometallic Compounds; D012710:Serum Albumin, Bovine; D004369:Pentetic Acid; D005340:Fibrinogen; D019786:Gadolinium DTPA