Isomer specific kinetics of dopamine beta-hydroxylase and arylsulfatase towards catecholamine sulfates.
Both isomers of epinephrine sulfate were synthesized, unequivocally identified by 1H-NMR and highly purified from catecholamines (less than 90 ppm). Bacterial as well as pig liver arylsulfatase A and B demonstrated a higher substrate turnover of epinephrine-4-sulfate, norepinephrine-4-sulfate and dopamine-4-sulfate as compared to the 3-sulfate isomers. The arylsulfatase B however, is less important for the deconjugation of these sulfoconjugates than arylsulfatase A. Since arylsulfatase A occurs in most human tissues, it might be of physiological significance in the deconjugation of the catecholamine sulfate isomers. Furthermore the kinetic data at pH 7.4 and 6.9 suggest the increased cleavage of the sulfate group, e.g. during exercise-induced acidosis. In contrast to results reported in the literature, dopamine sulfates were no substrates of dopamine beta-hydroxylase.
Strobel
G
G
Department of Pathophysiology and Sports Medicine, University of Heidelberg.
Werle
E
E
Weicker
H
H
D000818:Animals; D001192:Arylsulfatases / Q000378:metabolism*; D004298:Dopamine / Q000031:analogs & derivatives* / Q000378:metabolism; D004299:Dopamine beta-Hydroxylase / Q000378:metabolism*; D004837:Epinephrine / Q000031:analogs & derivatives* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007536:Isomerism; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D009638:Norepinephrine / Q000031:analogs & derivatives* / Q000378:metabolism; D013429:Sulfatases / Q000378:metabolism*; D013552:Swine
C022807:dopamine 4-O-sulfate; C036526:epinephrine sulfate; C017574:noradrenaline sulfate; D004299:Dopamine beta-Hydroxylase; D013429:Sulfatases; D001192:Arylsulfatases; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine