title: Comparison of UW solution and Euro-Collins solutions for cold preservation of human liver grafts.
abstract: University of Wisconsin solution, a new organ preservation medium, is reported to extend the period of cold storage. In order to evaluate the efficacy of UW solution in human liver preservation we compared 58 donor liver grafts preserved in Euro-Collins (EC) solution. All livers were harvested in a similar manner. Donor and recipient characteristics in the two groups were comparable. The mean preservation time of the UW solution was 11.5 +/- 4.2 hr (range 3-20 hr), significantly longer than the EC mean preservation time of 4.9 +/- 1.6 hr (2-9.6 hr) (P = 0.0001). Evaluation of mean postoperative liver function tests and coagulation factors on days 1-7 showed no statistical difference between the two groups. There was one primary graft nonfunction in the EC group and none with the UW organs. Hepatic artery thrombosis was similar in each group. The incidence of early retransplantation was similar. Three-month graft survival was 81% in the UW group vs. 73% in the EC group. Patient survival at three months was 87% with the UW organs and 84% with the EC organs. We conclude that cold storage of liver grafts in the UW solution has allowed for significantly longer preservation, permitting transplantation to be performed under semielective conditions and procurement of organs from much further distances. Grafts stored in UW solution perform as well as those stored in Euro-Collins, with no significant difference in liver function abnormalities postoperatively.
authors.affiliation: Department of Surgery, University of California, Los Angeles School of Medicine 90024.
authors.forename: K M
authors.initials: KM
authors.lastname: Olthoff
authors.forename: J M
authors.initials: JM
authors.lastname: Millis
authors.forename: D K
authors.initials: DK
authors.lastname: Imagawa
authors.forename: B J
authors.initials: BJ
authors.lastname: Nuesse
authors.forename: L J
authors.initials: LJ
authors.lastname: Derus
authors.forename: J T
authors.initials: JT
authors.lastname: Rosenthal
authors.forename: A L
authors.initials: AL
authors.lastname: Milewicz
authors.forename: R W
authors.initials: RW
authors.lastname: Busuttil
mesh_terms: D000241:Adenosine; D000293:Adolescent; D000410:Alanine Transaminase / Q000097:blood; D000493:Allopurinol; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D002140:California; D005260:Female; D005978:Glutathione; D006085:Graft Survival; D006801:Humans; D006982:Hypertonic Solutions; D007328:Insulin; D016031:Liver Transplantation; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D011887:Raffinose; D012996:Solutions; D016019:Survival Analysis; D013997:Time Factors
chemical_list: C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D007328:Insulin; D019188:Organ Preservation Solutions; D012996:Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose; D001663:Bilirubin