Ethylcholine aziridinium ion depletes acetylcholine and causes muscarinic receptor supersensitivity in rat retina.
Three weeks after a single intravitreal injection of ethylcholine aziridinium ion (AF64A), there was a significant depletion of retinal acetylcholine (ACh) content. The depletion was accompanied by an increase of [3H]quinuclidinyl benzilate binding sites and a greater than control conversion of myo-[3H]inositol to inositol monophosphate following incubation with ACh. These changes are consistent with receptor supersensitivity due to AF64A-induced denervation.
Moroi-Fetters
S E
SE
Department of Pathology, Ohio State University College of Medicine, Columbus 43210.
Neff
N H
NH
Hadjiconstantinou
M
M
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D001388:Aziridines / Q000494:pharmacology*; D002794:Choline / Q000031:analogs & derivatives* / Q000494:pharmacology; D007267:Injections; D007295:Inositol Phosphates / Q000378:metabolism; D008297:Male; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000378:metabolism*; D012160:Retina / Q000187:drug effects / Q000378:metabolism*; D014822:Vitreous Body
D001388:Aziridines; D007295:Inositol Phosphates; D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate; C044894:ethylcholine aziridinium; D002794:Choline; D000109:Acetylcholine