An enzyme-linked immunosorbent assay for the detection of antitetanus toxoid antibody using aluminum-absorbed coating antigen.
An enzyme-linked immunosorbent assay (ELISA) for the detection of antitetanus antibodies was developed using aluminum-absorbed tetanus toxoid as the coating antigen. The assay was tested by measuring antitetanus antibody levels in serum obtained from subjects before and after immunization with the aluminum-absorbed tetanus suspension. The specificity of the antibodies for tetanus antigen was tested both before and after antitetanus activity was removed with tetanus antigen-coated beads. Also, the activities of the sera were compared with those of a commercially available test. Our results indicated that the aluminum-absorbed tetanus suspension can be coated onto standard polystyrene ELISA plates and used to measure antibody titers to tetanus toxoid.
Thiele
G M
GM
Department of Internal Medicine, University of Nebraska Medical Center, Omaha 68105-1065.
Rogers
J
J
Collins
M
M
Yasuda
N
N
Smith
D
D
McDonald
T L
TL
D000042:Absorption; D000535:Aluminum; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D006801:Humans; D007075:Immunoglobulin M / Q000096:biosynthesis; D013745:Tetanus Toxoid / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007075:Immunoglobulin M; D013745:Tetanus Toxoid; D000535:Aluminum