Retrograde tubal transfer of human embryos.
This preliminary study was designed to evaluate retrograde cannulation of the Fallopian tubes up to the isthmo-interstitial junction using the new technique of tubal embryo stage transfer (TEST). Follicular aspiration was performed under the guidance of a vaginal ultrasound probe in 51 women treated with GnRH + HMG. The oocytes retrieved were inseminated in vitro with 50,000 motile spermatozoa and kept in Menezo B2 medium without serum, at 37 degrees C, in an atmosphere of air + 5% CO2. The eggs were checked 24 and 36 h after insemination. No fertilization occurred in 23 patients. Cleaved embryos were obtained in the 28 other patients. One to seven embryos at the 2-4-cell stage were transferred with the 'Baudelocque Black Catheter' (BBC) into one tube and spare embryos were frozen. Five pregnancies occurred after retrograde TEST, for a pregnancy rate of 9.8% per cycle and 17.9% per transfer. One patient has given birth to a normal full-term baby. One singleton and one twin pregnancy are ongoing (8 months in June 1989). The other two pregnancies were ectopic.
Risquez
F
F
Centre de PMA Baudelocque-St Vincent de Paul, Paris, France.
Boyer
P
P
Rolet
F
F
Magnani
M
M
Guichard
A
A
Cedard
L
L
Zorn
J R
JR
D000328:Adult; D002404:Catheterization; D002970:Cleavage Stage, Ovum; D004624:Embryo Transfer / Q000379:methods* / Q000592:standards; D005069:Evaluation Studies as Topic; D005187:Fallopian Tubes; D005260:Female; D006801:Humans; D011247:Pregnancy; D011271:Pregnancy, Ectopic