Low-density lipoprotein elevates intracellular calcium and pH in vascular smooth muscle cells and fibroblasts without mediation of LDL receptor.
Low-density lipoprotein (7 micrograms/ml) induced in the absence or in the presence of 7, 35, 70 micrograms/ml monoclonal antibodies against the specific Low-density lipoprotein receptor an elevation of intracellular Ca2+ from 105 to approximately 210 nM in vascular smooth muscle cells from rat aorta. Moreover, in both human cultured fibroblasts from normocholesterolemic individuals and from patients with familial hypercholesterolemia homozygote class 1, Low-density lipoprotein (7 micrograms/ml) induced a rise of free intracellular calcium and a biphasic change of intracellular pH. Low-density lipoprotein (1,7,15,30 micrograms/ml) had no significant influence on the phosphatidylinositol-turnover in vascular smooth muscle cells and fibroblasts. Since homozygote class 1 fibroblasts lack specific Low-density lipoprotein receptors, and as antibodies against this receptor did not attenuate the Low-density lipoprotein-induced elevation of cytosolic calcium and pH, we conclude that these intracellular changes are independent from the classical Low-density lipoprotein receptor.
Sachinidis
A
A
Department of Internal Medicine, University Hospital, Zürich, Switzerland.
Locher
R
R
Mengden
T
T
Vetter
W
W
D002118:Calcium / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007295:Inositol Phosphates / Q000032:analysis; D008077:Lipoproteins, LDL / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D011973:Receptors, LDL / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D007295:Inositol Phosphates; D008077:Lipoproteins, LDL; D011973:Receptors, LDL; D002118:Calcium