Cloning and sequencing of the ornithine carbamoyltransferase gene from Pachysolen tannophilus.
A fragment of DNA from a yeast Pachysolen tannophilus, bearing the ornithine carbamoyltransferase gene (OCTase, EC 2.1.3.3) has been cloned from a genomic library by functional complementation of the Escherichia coli OCT-negative mutant. The gene was located within the cloned segment of DNA and its coding sequence identified by DNA sequencing. This has indicated that P. tannophilus OCT gene encodes a 347 amino acid polypeptide, which shows 60% identity to the homologous Saccharomyces cerevisiae protein. The amino acid composition of its N-terminus indicates that this protein is translocated across the mitochondrial membrane. The gene can be expressed in E. coli as well as in S. cerevisiae. Comparison with other OCTases confirms a high degree of conservation among these proteins.
Skrzypek
M
M
Department of Genetics, University of Warsaw, Poland.
Borsuk
P
P
Maleszka
R
R
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D004271:DNA, Fungal / Q000235:genetics*; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009954:Ornithine Carbamoyltransferase / Q000235:genetics*; D004718:Saccharomycetales / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D003062:Codon; D004271:DNA, Fungal; D009954:Ornithine Carbamoyltransferase