Histopathologic study of the perilymph-suctioned labyrinth.
A small amount of perilymph was suctioned from the scala tympani through the round window membrane of guinea pigs. The animals either were killed immediately after the suctioning or were kept alive for 1 to 3 months. For morphologic study, the conventional celloidin embedding method was employed, and the temporal bones were serially sectioned. Various kinds and degrees of changes in the membranous labyrinth were observed: hydrops, collapse, and rupture. Loss of outer hair cells in the upper turns of the cochlea was noted in the 1- to 3-month recovery specimens. These findings are identical to those found in animals in which artificial perilymph was injected into the subarachnoid space in order to produce experimental perilymphatic fistula. After comparing morphologic changes of the membranous labyrinth following use of each method, we concluded that perilymph suctioning from the round window can be used as an animal model of perilymphatic fistula.
Hara
M
M
Department of Otolaryngology, University of Tokyo, Japan.
Nomura
Y
Y
Saito
K
K
D000818:Animals; D015834:Cochlear Diseases / Q000473:pathology; D004195:Disease Models, Animal; D007758:Ear, Inner / Q000293:injuries* / Q000473:pathology; D004487:Edema / Q000473:pathology; D005402:Fistula / Q000473:pathology*; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000473:pathology; D007759:Labyrinth Diseases / Q000473:pathology*; D007761:Labyrinthine Fluids; D010498:Perilymph; D012405:Round Window, Ear / Q000293:injuries; D012421:Rupture; D013396:Suction; D014722:Vestibule, Labyrinth / Q000473:pathology