[The use of a molecular DNA probe based on the cloned cytolysin gene for the identification of Legionella].
A molecular DNA probe has been obtained on the basis of recombinant plasmid pNIG carrying the Legionella cytolysin gene. The use of this probe for the identification of different Legionella species and other microorganisms has shown that it may serve as the species-specific marker of L. pneumophila. The probe has been used for the identification of new Legionella-like strains isolated from the environment.
Tartakovskiĭ
I S
IS
Nagaev
I G
IG
Marakusha
B I
BI
Zigangirova
N A
NA
Gintsburg
A L
AL
Smirnov
G B
GB
Prozorovskiĭ
S V
SV
D003001:Cloning, Molecular / Q000379:methods; D003603:Cytotoxins / Q000235:genetics*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D005798:Genes, Bacterial; D007875:Legionella / Q000235:genetics / Q000302:isolation & purification*; D010957:Plasmids / Q000235:genetics; D013045:Species Specificity
D003603:Cytotoxins; D015342:DNA Probes; D004269:DNA, Bacterial; D004274:DNA, Recombinant
Primenenie molekuliarnogo DNK-zonda na osnove klonirovannogo gena tsitolizina dlia identifikatsii legionell.