Differential poly(ADP-ribose) metabolism in repair-proficient and repair-deficient murine lymphoma cells.
The UV254nm-sensitive, tumorigenic murine lymphoma cell line LY-R undergoes spontaneous conversion into a UV254nm-resistant, non-tumorigenic LY-S subline after prolonged culture in vitro. Here we describe that this conversion leads to distinct changes in several features of intranuclear ADP-ribose polymer metabolism, which may contribute to the altered processing of DNA damage in these cells. The UV254nm-resistant LY-S cells contain 3-fold higher levels of ADP-ribose polymers than LY-R cells. The initial catabolic rate of degradation of these polymers is more than 6-fold higher in LY-S cells. UV254nm irradiation raises the catabolic rates of ADP-ribose polymers in both cell lines. As a consequence, the polymer half-lives decrease from 15 min to 4 min in LY-S cells, and from 96 min to 19 min in LY-R cells. In addition, the rapidly turning over fraction of polymers is much larger in the UV254nm-resistant LY-S cells. These data suggest that the catabolism of poly(ADP-ribose) may be an important factor in the biological expression of DNA damage.
Kleczkowska
H E
HE
Institute of Nuclear Chemistry and Technology, Warsaw, Poland.
Szumiel
I
I
Althaus
F R
FR
D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D002846:Chromatography, Affinity; D004260:DNA Repair; D008223:Lymphoma / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D011064:Poly Adenosine Diphosphate Ribose / Q000302:isolation & purification / Q000378:metabolism*; D011836:Radiation Tolerance / Q000235:genetics; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
D001549:Benzamides; D009702:Nucleoside Diphosphate Sugars; D011064:Poly Adenosine Diphosphate Ribose; C037689:benzamide