title: Series distribution of mucociliary clearance of magnetized iron particles in anesthetized dogs.
abstract: Clearance of magnetic iron particles placed in the trachea, peripheral airway and alveoli was studied in anesthetized dogs. A retrograde catheter was inserted into a peripheral airway 2 mm in diameter. A thin vinyl tube was inserted into the peripheral airway from the chest wall through the retrograde catheter and 0.05 ml of saline containing 30 mg of iron oxide (Fe3O4) was instilled into either the peripheral airway or the trachea. Further, 30 mg of Fe3O4 solution was instilled with an injection needle into the alveoli. After magnetization from outside the chest wall, remanent magnetic fields (RMF) generated in the trachea, peripheral airway and alveoli were sequentially measured with a flux-gate magnetometer. The decay of RMF immediately after sequential magnetization shows clearance of Fe3O4 particles. Relaxation of RMF just before sequential magnetization is related to random misalignment of the particles. The fastest clearance was observed in the trachea, followed by the peripheral airway and the slowest was that in the alveoli. Exposure to isoproterenol resulted in increased clearance in the trachea and peripheral airway. However, relaxation was of similar magnitude and was not altered by isoproterenol throughout the entire airway. These results suggest that mechanisms responsible for clearance and relaxation in vivo are different and that clearance accelerates toward the central airway from alveoli and relaxation may be due to properties of the airway surface lining layer.
authors.affiliation: First Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Miyano
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
authors.forename: M
authors.initials: M
authors.lastname: Yamaya
authors.forename: T
authors.initials: T
authors.lastname: Fukushima
authors.forename: K
authors.initials: K
authors.lastname: Sekizawa
authors.forename: H
authors.initials: H
authors.lastname: Sasaki
authors.forename: T
authors.initials: T
authors.lastname: Takishima
mesh_terms: D000818:Animals; D004285:Dogs; D005290:Ferric Compounds / Q000493:pharmacokinetics*; D008280:Magnetics; D009079:Mucociliary Clearance / Q000502:physiology*; D011650:Pulmonary Alveoli / Q000502:physiology*; D014132:Trachea / Q000502:physiology*
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide