Carbohydrates as a tool for oriented immobilization of antigens and antibodies.
A biospecific sorbent for the isolation of ovalbumin antibodies was prepared by coupling of ovalbumin via its periodate-oxidized carbohydrate moiety to bead cellulose modified with adipic acid dihydrazide. The anti-ovalbumin IgG fraction isolated on this sorbent from immune rabbit serum contained only antibodies against protein determinants of ovalbumin. Thus, when these IgG were immobilized through their carbohydrate moieties to cellulose beads it became possible to prepare a biospecific sorbent for concanavalin A by oriented adsorption of ovalbumin. Ovalbumin was specifically adsorbed via its protein moiety and its carbohydrate part remained free for interaction with concanavalin A.
Turková
J
J
Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Prague.
Petkov
L
L
Sajdok
J
J
Kás
J
J
Benes
M J
MJ
D000327:Adsorption; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D002241:Carbohydrates; D002846:Chromatography, Affinity; D003208:Concanavalin A / Q000032:analysis / Q000302:isolation & purification*; D007106:Immune Sera; D007074:Immunoglobulin G / Q000302:isolation & purification*; D008297:Male; D010047:Ovalbumin / Q000276:immunology / Q000302:isolation & purification; D011817:Rabbits
D000906:Antibodies; D002241:Carbohydrates; D007106:Immune Sera; D007074:Immunoglobulin G; D003208:Concanavalin A; D010047:Ovalbumin