Acid lipase in rat intestinal mucosa: physiological parameters.
Previous studies have shown that up to a half of infused triacylglycerol does not exit the intestine via lymphatics. This suggests the presence of a mucosal lipase which could provide fatty acids for potential transport via the portal vein. The present study describes an acid-active lipase in rat intestinal mucosa. Acid lipase was assayed using a glyceryl tri[14C]oleate emulsion (pH 5.8). Mucosal homogenates were differentially centrifuged to yield cellular organelles and cytosol. Cells were sequentially released from villi using citrate and EDTA. The enzyme was found to be most active in the proximal quarter intestine and in the upper third of villi. Its greatest activity was in the lysosomal fraction. Esophageal diversion demonstrated that lingual lipase was not the precursor of the mucosal acid lipase. Bile salts stimulated activity 3- to 5-fold, but other neutral or anionic detergents were inhibitory. Of the detergents tested, taurocholate at super critical micellar concentrations could restore activity only with SDS. Sepharose 6B chromatography suggested that the enzyme partitioned into an SDS and taurocholate mixed micelle. We conclude that mucosal acid lipase is a distinct, intrinsic enzyme of the intestinal mucosa. It is predominantly lysosomal in origin. The location of its greatest activity in the villus tips of the proximal intestine suggests that it is potentially involved in mucosal triacylglycerol disposal.
Rao
R H
RH
Department of Medicine, University of Tennessee, Memphis 38163.
Mansbach
C M
CM
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001647:Bile Acids and Salts / Q000502:physiology; D002738:Chloroquine / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000033:anatomy & histology / Q000201:enzymology*; D008049:Lipase / Q000502:physiology*; D050356:Lipid Metabolism; D008297:Male; D008744:Methylamines / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D001647:Bile Acids and Salts; D008744:Methylamines; D002738:Chloroquine; C027451:methylamine; D008049:Lipase; D000135:Acid Phosphatase