The influence of nifedipine and mioflazine on mitochondrial calcium overload in normoxic and ischaemic guinea-pig hearts.
The influence of nifedipine (20 nM) and mioflazine (300 nM), i.e. concentrations inducing a 60-70% recovery of cardiac function during reperfusion of globally ischaemic guinea-pig working hearts, on the mitochondrial calcium content was investigated in normoxic, globally ischaemic and reperfused globally ischaemic guinea-pig working hearts. Mitochondrial calcium was determined electronmicroscopically with oxalate-pyroantimonate method. In normoxic hearts both nifedipine and mioflazine reduced the mitochondrial calcium content. Global ischaemia for 45 min and subsequent reperfusion for 25 min resulted in a pronounced mitochondrial calcium overload and damage to the cellular structure. In ischaemic and in reperfusion hearts the drugs maintained mitochondrial calcium at pre-ischaemic levels and decreased the damage to the cellular structure.
Hugtenburg
J G
JG
Department of Pharmacotherapy/Pharmacology, Faculty of Medicine, University of Amsterdam, The Netherlands.
Van Voorst
M J
MJ
Van Marle
J
J
Mathy
M J
MJ
Beckeringh
J J
JJ
Van Veen
H A
HA
Hendriks
M G
MG
Van Zwieten
P A
PA
D000818:Animals; D000965:Antimony; D002118:Calcium / Q000378:metabolism*; D002317:Cardiovascular Agents / Q000494:pharmacology*; D003327:Coronary Disease / Q000378:metabolism*; D006168:Guinea Pigs; D006706:Homeostasis / Q000187:drug effects; D066298:In Vitro Techniques; D008929:Mitochondria, Heart / Q000187:drug effects / Q000378:metabolism*; D015425:Myocardial Reperfusion; D009543:Nifedipine / Q000494:pharmacology*; D010070:Oxalates; D010879:Piperazines / Q000494:pharmacology*
D002317:Cardiovascular Agents; D010070:Oxalates; D010879:Piperazines; C009133:pyroantimonate; C041518:mioflazine; D000965:Antimony; D009543:Nifedipine; D002118:Calcium