The role of the viroid central conserved region in cDNA infectivity.
The effect of sequence duplication upon the infectivity of plasmid DNAs containing monomeric tomato apical stunt viroid cDNAs has been determined. Two factors appear to control the specific infectivity of the different plasmid constructions tested: the presence of a subset of a palindromic sequence located within the central conserved region and the orientation of the viroid cDNA within the recombinant plasmid. Deletions which disrupt the integrity of the putative processing site abolished cDNA infectivity, a result that is consistent with the involvement of this site in the cleavage/ligation of viroid RNAs during replication.
Candresse
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Plant Sciences Institute, Beltsville Agricultural Research Center, Maryland 20705.
Diener
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TO
Owens
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RA
D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D010942:Plant Viruses / Q000235:genetics* / Q000472:pathogenicity; D010957:Plasmids; D015183:Restriction Mapping; D014772:Viroids / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence
D004279:DNA, Viral