Evaluation of partially purified salivary gland allergens from the Australian paralysis tick Ixodes holocyclus in diagnosis of allergy by RIA and skin prick test.
Two diagnostic assays that detect IgE specific to I. holocyclus were developed using purified sources of allergens. Salivary gland extract was superior to whole body extract in both the radioimmunoassay and the skin prick test. Of the partially purified salivary gland allergens, the 28-KD protein fraction gave the most promising results.
Gauci
M
M
CSIRO, Division of Tropical Animal Production, Long Pocket Laboratories, Queensland, Australia.
Loh
R K
RK
Stone
B F
BF
Thong
Y H
YH
D000485:Allergens / Q000302:isolation & purification*; D000818:Animals; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis*; D007073:Immunoglobulin E / Q000276:immunology; D011863:Radioimmunoassay; D012469:Salivary Glands / Q000276:immunology*; D012882:Skin Tests; D013987:Ticks / Q000032:analysis / Q000276:immunology*; D014020:Tissue Extracts / Q000276:immunology
D000485:Allergens; D014020:Tissue Extracts; D007073:Immunoglobulin E