title: Crystallographic characterization of a Cu,Zn superoxide dismutase from Photobacterium leiognathi.
abstract: Crystals of a copper-zinc superoxide dismutase from Photobacterium leiognathi, a luminescent marine bacterium that is the species-specific symbiont of the ponyfish, have been obtained from 2-methyl-2,4-pentanediol solutions. The space group was determined using screenless small-angle precession photographs, and was confirmed by analyzing area detector diffraction data with the XENGEN programs for indexing and refinement. The crystals are monoclinic, space group C2 (a = 126.4 A, b = 87.0 A, c = 44.4 A, beta = 92.8 A), and have two 32,000 Mr dimers per asymmetric unit. The crystals diffract to at least 2.7 A resolution, are resistant to radiation damage, and are suitable for determination of the structure by X-ray diffraction.
authors.affiliation: Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
authors.forename: S M
authors.initials: SM
authors.lastname: Redford
authors.forename: D E
authors.initials: DE
authors.lastname: McRee
authors.forename: E D
authors.initials: ED
authors.lastname: Getzoff
authors.forename: H M
authors.initials: HM
authors.lastname: Steinman
authors.forename: J A
authors.initials: JA
authors.lastname: Tainer
mesh_terms: D010776:Photobacterium / Q000201:enzymology*; D013482:Superoxide Dismutase; D014961:X-Ray Diffraction
chemical_list: D013482:Superoxide Dismutase