Pyridoxal 5'-phosphate and pyridoxamine 5'-phosphate concentrations in blood and tissues of mice fed ethanol-containing liquid diets.
The effects of chronic ethanol administration on vitamin B-6 metabolism were studied in female Long-Sleep (LS) and Short-Sleep (SS) mice. Animals were fed an ethanol containing liquid diet (AIN-76) for four weeks. Concentration of ethanol in the diet increased from 10 to 25% ethanol-derived calories (EDC) during weeks 1-3 and was maintained at 30% EDC for 1 additional week. We measured concentrations of pyridoxal 5'-phosphate (PLP) in plasma, erythrocytes and whole blood, and liver and brain PLP and pyridoxamine 5'-phosphate (PMP) in ethanol-fed and pair-fed control mice. Chronic ethanol administration significantly increased PMP and total (PLP + PMP) levels in the liver of SS mice. In LS mice ethanol feeding significantly decreased PMP and total (PLP + PMP) levels in the brain, but these values were still within normal limits. These results suggest that both control and ethanol-containing liquid diets are nutritionally adequate with respect to vitamin B-6, and that chronic ethanol administration does not adversely affect vitamin B-6 metabolism in adult mice.
Leibman
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D
Institute for Behavioral Genetics, University of Colorado, Boulder 80309-0447.
Furth-Walker
D
D
Smolen
T N
TN
Smolen
A
A
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002149:Energy Intake; D004912:Erythrocytes / Q000378:metabolism; D000431:Ethanol / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008817:Mice, Mutant Strains; D010542:Peromyscus; D011732:Pyridoxal Phosphate / Q000097:blood* / Q000378:metabolism; D011733:Pyridoxamine / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism; D011736:Pyridoxine / Q000378:metabolism
D000431:Ethanol; D011732:Pyridoxal Phosphate; D011733:Pyridoxamine; D011736:Pyridoxine; C010627:pyridoxamine phosphate