Injury induced by fatty acids or bile acid in isolated human colonocytes prevented by calcium.
Measurement of the modulation of the growth fraction of isolated normal colonocytes from adult subjects in primary monolayer culture was used as a sensitive quantitative assay to evaluate toxic effects of several endogenous compounds found within the colon. This assay was used to study the role of CaCl2 in blocking cell injury. When added simultaneously with the injurious agent, 5-10 mM CaCl2 blocked the toxicity of physiological concentrations of deoxycholic acid, oleic acid, palmitic acid and linoleic acid.
Buset
M
M
Hospital Erasme, Université Libre de Bruxelles, Belgium.
Galand
P
P
Lipkin
M
M
Winawer
S
S
Friedman
E
E
D002122:Calcium Chloride / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003106:Colon / Q000166:cytology / Q000187:drug effects; D003840:Deoxycholic Acid / Q000037:antagonists & inhibitors* / Q000633:toxicity; D005230:Fatty Acids, Nonesterified / Q000037:antagonists & inhibitors* / Q000633:toxicity; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects*; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000037:antagonists & inhibitors; D019301:Oleic Acid; D009829:Oleic Acids / Q000037:antagonists & inhibitors; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000037:antagonists & inhibitors
D005230:Fatty Acids, Nonesterified; D008041:Linoleic Acids; D009829:Oleic Acids; D010169:Palmitic Acids; D003840:Deoxycholic Acid; D019301:Oleic Acid; D019308:Palmitic Acid; D019787:Linoleic Acid; D002122:Calcium Chloride