Increased yield of antibody-producing murine spleen cell hybridomas from fusions cultured in medium supplemented with mouse serum.
The number of monoclonal antibody-producing murine hybridomas were increased 7-10-fold in three separate fusions when supplementing the FBS-containing, or serum-free, growth factor-containing, culture medium with 5% normal mouse serum. The beneficial effect was seen in fusions of A/J and 129GIX+ spleen cells, from primary and secondary responses, and when using SP2/0 or P3X63Ag8U.1 as the fusion partner.
Persson
M A
MA
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
Lerner
R A
RA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001790:Blood Physiological Phenomena; D002478:Cells, Cultured; D003470:Culture Media; D006825:Hybridomas / Q000276:immunology*; D051379:Mice; D013154:Spleen / Q000276:immunology
D000911:Antibodies, Monoclonal; D003470:Culture Media