title: Cell type dependent activation of poly (ADP-ribose) synthesis following treatment with etoposide.
abstract: Treatment of human non-lymphoid cell lines, HL-60 and U937, with etoposide stimulated poly (ADP-ribose) synthesis three- to fourfold, whereas no significant effects were observed in the lymphoid cell lines, Molt4 and CEM. This was confirmed by either an increased uptake of radio-labelled NAD into the acid-insoluble fraction or a fall in cellular NAD levels, which was counteracted by 3-aminobenzamide, an inhibitor of poly(ADP-ribose) polymerase. On the other hand, another DNA damaging agent, N-methyl-N'-nitro-N-nitrosoguanidine augmented poly(ADP-ribose) synthesis equally in both cell types. These results taken together indicate that the activation of poly(ADP-ribose) synthesis following exposure to etoposide is a cell type specific phenomenon.
authors.affiliation: Department of Pediatrics, Kyoto University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kubota
authors.forename: A
authors.initials: A
authors.lastname: Tanizawa
authors.forename: H
authors.initials: H
authors.lastname: Hashimoto
authors.forename: T
authors.initials: T
authors.lastname: Shimizu
authors.forename: T
authors.initials: T
authors.lastname: Takimoto
authors.forename: T
authors.initials: T
authors.lastname: Kitoh
authors.forename: Y
authors.initials: Y
authors.lastname: Akiyama
authors.forename: H
authors.initials: H
authors.lastname: Mikawa
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000187:drug effects; D004249:DNA Damage; D005047:Etoposide / Q000494:pharmacology*; D006801:Humans; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009702:Nucleoside Diphosphate Sugars / Q000096:biosynthesis*; D011064:Poly Adenosine Diphosphate Ribose / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
chemical_list: D009702:Nucleoside Diphosphate Sugars; D008769:Methylnitronitrosoguanidine; D011064:Poly Adenosine Diphosphate Ribose; D005047:Etoposide; D004247:DNA