[SDI test using MTT for the evaluation of drug sensitivities of gastrointestinal cancer cells].
The Succinic Dehydrogenase Inhibition test (SDI test) has been widely used for a long time to evaluate the sensitivities of cancer cells to anticancer agents. Recently, the techniques for tissue culture have progressed and the ability to make formazan improved through the use of MTT in this assay instead of TTC. Therefore, we modified SDI test in order to evaluate the drug response of gastrointestinal cancer and applied it in a clinical study. For the modification of the assay, we found it necessary to culture the cancer cells for three days and bring those cells in contact with MTT over a period of one hour. When we examined the sensitivities to anticancer agents at four different drug concentrations, if the cells were sensitive to the drugs, the optical densities were reduced according to the elevation of the drug concentrations. Further, we measured the drug concentrations in the blood of nude mice after the administration of anticancer drugs in the subrenal capsule assay which we developed previously. The drug concentration levels in the mice indicate the adequate drug dosage necessary to be effective in SDI test.
Idota
C
C
2nd Department of Surgery, Nagoya University School of Medicine.
Yamauchi
M
M
Takagi
H
H
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D005770:Gastrointestinal Neoplasms / Q000188:drug therapy*; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D013370:Subrenal Capsule Assay; D013385:Succinate Dehydrogenase / Q000037:antagonists & inhibitors; D013778:Tetrazolium Salts; D013844:Thiazoles
D000970:Antineoplastic Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; D013385:Succinate Dehydrogenase; C022616:thiazolyl blue