DNA alterations in prostatic adenocarcinoma and benign prostatic hyperplasia: detection by DNA fingerprint analyses.
DNA fingerprinting can be utilized to examine a large number of autosomal loci throughout the human genome. Alterations in banding patterns observed on DNA fingerprint analyses reflect DNA alterations ranging from single base changes to complex chromosomal rearrangements. In this report, we describe the application of this technique to prostatic adenocarcinoma (CAP) and benign prostatic hyperplasia (BPH). The majority of CAP cases (12 of 14) displayed alterations in at least 1 of the approximately 30 resolvable bands obtained by fingerprint analyses when compared with DNA obtained from peripheral white blood cells. Unexpectedly, 5 of the 12 cases of BPH examined revealed at least 1 identifiable band alteration in the prostatic tissue. These findings demonstrate the usefulness of fingerprint analyses in the examination of cancer-associated genetic alterations. They also suggest the presence of observable genetic alterations in BPH.
White
J J
JJ
Gerontology Research Center, National Institute on Aging, Baltimore, MD 21224.
Neuwirth
H
H
Miller
C D
CD
Schneider
E L
EL
D000230:Adenocarcinoma / Q000235:genetics*; D015139:Blotting, Southern; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000235:genetics*; D006801:Humans; D006965:Hyperplasia; D008297:Male; D009710:Nucleotide Mapping; D011467:Prostate / Q000473:pathology*; D011471:Prostatic Neoplasms / Q000235:genetics*
D015342:DNA Probes; D004273:DNA, Neoplasm