[The cells of murine sarcoma PC-103 induced by a polymeric plate secret transforming growth factor alpha].
It was shown previously that cells of sarcoma PS-103 induced in mouse by subcutaneous transplantation of plastic film produce growth-stimulating activity. In this communication clone 3 sb, isolated from sarcoma PS-103, was studied. Growth factor produced by the cells of this clone stimulated proliferation of quasi-normal (3T3, NRK) cells and transformed cells both in monolayer and in semi-solid medium. It was shown by gel filtration chromatography that the main growth-stimulating activity migrated with the protein fraction m.m. 10-15 kDa. The addition of this growth factor to the culture medium significantly (90%) inhibited 125I-EGF (epidermal growth factor) binding by A-431 cells. These data suggest that the growth factor produced by the studied tumor cells is transforming growth factor alpha.
Stavrovskaia
A A
AA
Stromskaia
T P
TP
Brodskaia
R M
RM
Stoĭka
R S
RS
Sushel'nitskiĭ
S I
SI
Garas'ko
S I
SI
Shafranskaia
G S
GS
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002461:Cell Line, Transformed; D002478:Cells, Cultured / Q000187:drug effects; D002999:Clone Cells / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000037:antagonists & inhibitors; D051379:Mice; D008806:Mice, Inbred AKR; D008808:Mice, Inbred CBA; D008970:Molecular Weight; D011143:Polyvinyl Chloride; D012513:Sarcoma, Experimental / Q000139:chemically induced / Q000378:metabolism*; D015291:Transforming Growth Factors / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
D004815:Epidermal Growth Factor; D015291:Transforming Growth Factors; D011143:Polyvinyl Chloride
Kletki sarkomy PS-103 mysheĭ, indutsirovannoĭ polimernoĭ plastinkoĭ, sekretiruiut transformiruiushchiĭ faktor rosta al'fa.