Reactivity of coagulase-negative staphylococci isolated from cow and goat milk with monoclonal antibodies to Staphylococcus aureus capsular polysaccharide types 5 and 8.
Monoclonal antibodies to Staphylococcus aureus capsular polysaccharide types 5 and 8 were used in an enzyme-linked immunosorbent assay to serotype 74 and 42 coagulase-negative isolates from cow and goat milk, respectively. Eighteen (15.5%) isolates were typable: 13 Staphylococcus haemolyticus, 1 S. hyicus, 1 S. simulans, and 1 S. warneri from bovine origin and 2 S. lentus from caprine origin. Type 5 was predominant, accounting for about 89% of typable isolates. Reactivity with monoclonal antibodies varied considerably according to isolates. The significance and the potential importance of these findings are discussed.
Poutrel
B
B
Station de Pathologie de la Reproduction, Institut National de la Recherche Agronomique, Nouzilly, France.
Mendolia
C
C
Sutra
L
L
Fournier
J M
JM
D000818:Animals; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D016667:Bacterial Capsules; D002417:Cattle; D003030:Coagulase / Q000378:metabolism; D006041:Goats; D008892:Milk / Q000382:microbiology*; D011135:Polysaccharides, Bacterial / Q000145:classification / Q000276:immunology; D012703:Serotyping; D013211:Staphylococcus aureus / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D003030:Coagulase; D011135:Polysaccharides, Bacterial; C054106:capsular polysaccharide, type 5, Staphylococcus aureus