title: DNA sequencing, automation, and the human genome.
abstract: DNA sequencing is one of the key analytical operations of modern molecular biology and a crucial element of biotechnology. The principles of DNA sequencing and details of the technologies of both manual, radioisotope-based and automated, fluorescence-based approaches are described. The goals and rationale of the Human Genome Initiative are discussed along with implications for future sequencing technologies. Finally, a glimpse of emerging DNA sequencing technologies is offered.
authors.affiliation: E. I. du Pont de Nemours and Co., Inc., Central Research and Development Department, Wilmington, Delaware 19880-0328.
authors.forename: G L
authors.initials: GL
authors.lastname: Trainor
mesh_terms: D001322:Autoanalysis; D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data
chemical_list: D004247:DNA