[Biological evaluation of extracellular superoxide dismutase observed in a state of acute gastric mucosal lesions].
In the experimental stress ulcer models of guinea pig superoxide dismutase-like plasma substance increased in plasma. Upon chromatography on heparin-Sepharose, this substance was separated into three fractions; A, without affinity for heparin; B, with intermediate affinity for heparin; C, with relatively strong heparin affinity, and the fraction C was specifically increased by a stress and a heparin injection. Analysis by a polyacrylamide gel electrophoresis (PAGE) of the fraction C revealed SOD activity, whose molecular weight was 135 K in a native state. The fraction C seemed to be the same as extracellular superoxide dismutase (EC-SOD) reported by Marklund. Assuming to the releasing potency of heparin, the major part of EC-SOD in the vasculature was most likely located on endothelial-cell surface. A pathophysiological role of EC-SOD released into plasma seemed to be an indicator of endothelial-cell damage by free radicals of endothelial cells and polymorphonuclear neutrophils (PMNs).
Ohara
T
T
Third Department of Internal Medicine, School of Medicine, Tohoku University, Sendai, Japan.
Asaki
S
S
Toyota
T
T
D000208:Acute Disease; D000818:Animals; D004730:Endothelium, Vascular / Q000201:enzymology*; D005110:Extracellular Space / Q000201:enzymology; D005753:Gastric Mucosa / Q000473:pathology*; D006168:Guinea Pigs; D008297:Male; D013276:Stomach Ulcer / Q000201:enzymology / Q000473:pathology; D013312:Stress, Physiological / Q000503:physiopathology; D013482:Superoxide Dismutase / Q000302:isolation & purification / Q000378:metabolism*
D013482:Superoxide Dismutase