A harvesting method of monocytes/macrophages derived from bone marrow and their characteristics--special reference to the effect of 1,25-dihydroxyvitamin D3 on monocytes/macrophages.
We have investigated the relationship between monocytes/macrophages and bone metabolism with the in vitro study. In the present study monocytes/macrophages derived from rat bone marrow were harvested and their phenotype was examined. Collected cells exhibited many characteristics of monocytes/macrophages at various points. In addition, 1,25-dihydroxyvitamin D3(D3), one of the osteotropic hormones was found to alter the activities of acid phosphatase (AcPase) and lysozyme in the media and also stimulate alkaline phosphatase (AlPase) positive cells to increase in number.
Takagi
M
M
Department of Orthopedic Surgery, Yamagata University School of Medicine, Japan.
Suda
A
A
Watanabe
Y
Y
Saotome
K
K
D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells; D002117:Calcitriol / Q000494:pharmacology*; D002469:Cell Separation / Q000379:methods; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D009000:Monocytes / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D009113:Muramidase / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D051381:Rats
D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D009113:Muramidase; D002117:Calcitriol