title: African swine fever virus: purification of capsomeres released by lipase.
abstract: The virus capsomeres of the outer and inner layers of capsids were effectively released simultaneously from purified virions by lipase digestion and were purified by a linear gradient ultracentrifugation. The capsid consisted of an array of double layers of uniformly arranged individual capsomeres where a lipid(s) served as a matrix in between the capsomeres.
authors.affiliation: Plum Island Animal Disease Center, USDA, Greenport, New York 11944.
authors.forename: I C
authors.initials: IC
authors.lastname: Pan
authors.forename: Y
authors.initials: Y
authors.lastname: Murakami
authors.forename: J C
authors.initials: JC
authors.lastname: Saiz
mesh_terms: D000358:African Swine Fever Virus / Q000032:analysis* / Q000648:ultrastructure; D002213:Capsid / Q000302:isolation & purification* / Q000648:ultrastructure; D007497:Iridoviridae / Q000032:analysis*; D008049:Lipase; D008854:Microscopy, Electron; D014771:Virion / Q000032:analysis / Q000648:ultrastructure
chemical_list: D008049:Lipase