title: An improved method for complement subcomponent C1R typing.
abstract: An improved method has been developed for the reliable classification of different C1R genetic variant forms from human serum or plasma. The method combines the use of neuraminidase-digested samples followed by monodimensional isoelectric focusing in the pH range 5 to 8 followed by immunoblotting. The method yields a simple pattern, with one major band in homozygote and two major bands in heterozygote cases.
authors.affiliation: Department of Human Genetics, Graduate School of Public Health, University of Pittsburgh, PA.
authors.forename: M I
authors.initials: MI
authors.lastname: Kamboh
authors.forename: R E
authors.initials: RE
authors.lastname: Ferrell
mesh_terms: D001788:Blood Grouping and Crossmatching / Q000379:methods*; D015923:Complement C1r / Q000032:analysis*; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015151:Immunoblotting; D007525:Isoelectric Focusing; D009439:Neuraminidase / Q000378:metabolism; D010641:Phenotype
chemical_list: D009439:Neuraminidase; D015923:Complement C1r