Analysis of anabolic steroids using GC/MS with selected ion monitoring.
This study describes the use of gas chromatography/mass spectrometry with selected ion monitoring to screen 18 anabolic steroids banned by the International Olympic Committee. These anabolic steroids are analyzed in two fractions depending on their excretion pattern: nonconjugated (free) or conjugated fraction. The wet procedure of extracting steroids from urine consists of an initial isolation of lipophilic compounds on a column packed with Amberlite XAD-2 resin, followed by enzymatic hydrolysis with beta-glucuronidase from Escherichia coli. After extraction, the hydrolyzed steroids are derivatized to the corresponding trimethylsilyl ethers. The derivatized steroids are analyzed by gas chromatography/mass spectrometry with selected ion monitoring of their characteristic ions. It takes 12 and 26 min to run GC/MS and edit the raw data for nonconjugated and conjugated fractions respectively.
Chung
B C
BC
Doping Control Center, Korea Institute of Science & Technology, Cheongryang, Seoul.
Choo
H Y
HY
Kim
T W
TW
Eom
K D
KD
Kwon
O S
OS
Suh
J
J
Yang
J
J
Park
J
J
D045930:Anabolic Agents / Q000652:urine*; D004300:Doping in Sports; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D015813:Substance Abuse Detection / Q000379:methods*
D045930:Anabolic Agents