Differential localization of microsomal mixed function oxidase activity in periportal and perivenous hepatocytes isolated from rat liver.
1. The activity per mg of microsomal protein of aminopyrine N-demethylase was higher in perivenous (PV) than in periportal (PP) hepatocytes of rat, but when it was expressed per cytochrome P-450 content the difference in the activity was not significant. 2. The activity of 7-ethoxycoumarin O-deethylase, when expressed per mg protein and per P-450 content, was significantly higher in PV than in PP cells. 3. The activities of dimethylnitrosamine(DMNA) N-demethylase and aniline p-hydroxylase were not significantly different between two subpopulations of isolated hepatocytes when either expressed per mg protein or per P-450 content.
Yamazaki
H
H
Department of Pharmacology, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Japan.
Nishiguchi
K
K
Serasinghe
P
P
Nakanishi
S
S
D000632:Aminopyrine / Q000378:metabolism; D000814:Aniline Compounds / Q000378:metabolism; D000818:Animals; D003374:Coumarins / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004128:Dimethylnitrosamine / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D009245:NADH Dehydrogenase / Q000378:metabolism; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D000814:Aniline Compounds; D003374:Coumarins; D000632:Aminopyrine; C017299:7-ethoxycoumarin; D003577:Cytochrome P-450 Enzyme System; D000587:Oxidoreductases Acting on CH-NH Group Donors; C019668:amine oxidase, mixed function; D009245:NADH Dehydrogenase; D004128:Dimethylnitrosamine; C023650:aniline