Rapid procedure to determine the DNA base composition from small amounts of gram-positive bacteria.
A universal rapid procedure to determine the DNA base composition (mol% guanine + cytosine) of Gram-positive bacteria is described. Cells of Gram-positive bacteria were lysed with achromo-peptidase and the mol% G + C of their DNAs were determined by using high performance liquid chromatography. One ml of a Gram-positive bacterial suspension which matched MacFarland No. 3 standard turbidity was sufficient to determine the mol% G + C within 3 h.
Ezaki
T
T
Department of Microbiology, Gifu University School of Medicine, Japan.
Saidi
S M
SM
Liu
S L
SL
Hashimoto
Y
Y
Yamamoto
H
H
Yabuuchi
E
E
D001482:Base Composition; D002851:Chromatography, High Pressure Liquid; D004269:DNA, Bacterial / Q000032:analysis*; D006094:Gram-Positive Bacteria / Q000032:analysis*; D012697:Serine Endopeptidases
D004269:DNA, Bacterial; D012697:Serine Endopeptidases; C036813:lysyl endopeptidase