On-line high-performance liquid chromatography for the determination of cephalosporin C and by-products in complex fermentation broths.
A fully automated high-performance liquid chromatographic system was developed for optimization of cephalosporin C production. Using a simple, isocratic, reversed-phase method, cephalosporin C, deacetylcephalosporin C, deacetoxycephalosporin C, penicillin N, methionine and its decomposition product 2-hydroxy-4-methylmercaptobutyric acid were determined. The method worked reliably for 250 h and supplied information on the influence of many parameters of the process.
Holzhauer-Rieger
K
K
Institut für Technische Chemie, Universität Hannover, F.R.G.
Zhou
W
W
Schügerl
K
K
D002511:Cephalosporins / Q000032:analysis* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005285:Fermentation
D002511:Cephalosporins; C025163:cephalosporin C