title: Microcirculation of the pancreas. A quantitative study of physiology and changes in pancreatitis.
abstract: A rabbit model was designed to study the microcirculation of the pancreas with special reference to changes occurring during acute pancreatitis. Intravital microscopy was used in conjunction with video techniques allowing for continuous observation and off-line evaluation of microvessel diameters and blood cell velocities. Based on the microvessel geometry a functional microvascular unit could be defined at the level of the pancreatic lobule consisting of intralobular arteries and veins and an arcade-like preferential pathway framing the capillary network. Experimental acute pancreatitis resulted in immediate leakage of the macromolecular plasma marker (FITC-Dextran 70) from the microvasculature suggesting increased permeability. In contrast to control conditions, pancreatic capillaries were excluded from the circulation during acute pancreatitis starting 30 min after induction with only single capillaries remaining perfused after 3 hours. At the same time, there was constant blood flow through the preferential pathways representing shunt perfusion.
authors.affiliation: Department of General Surgery, University of Heidelberg, FRG.
authors.forename: E
authors.initials: E
authors.lastname: Klar
authors.forename: B
authors.initials: B
authors.lastname: Endrich
authors.forename: K
authors.initials: K
authors.lastname: Messmer
mesh_terms: D000208:Acute Disease; D000818:Animals; D002196:Capillaries / Q000473:pathology / Q000503:physiopathology; D008991:Monitoring, Physiologic; D010179:Pancreas / Q000098:blood supply*; D010195:Pancreatitis / Q000473:pathology / Q000503:physiopathology*; D011817:Rabbits; D014741:Video Recording