title: Members of the alpha-amylase inhibitors family from wheat endosperm are major allergens associated with baker's asthma.
abstract: We have identified the major antigens or IgE binding components from wheat flour. Thirty-five sera from patients with baker's asthma were used to analyze the reaction with wheat salt-soluble proteins. We found a 15 kDa SDS-PAGE band which reacted with all sera tested. Purified members of the alpha-amylase inhibitor family, which are the main components of the 15 kDa band, were recognized by specific IgE when tested with a pool of reactive sera. Immunodetection after two-dimensional electrophoretic fractionation of crude inhibitor preparations from wheat endosperms also detected several inhibitor subunits as major low-molecular-weight allergens.
authors.affiliation: Departamento de Bioquimica, ETS Ingenieros Agrónomos, Madrid, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Gómez
authors.forename: E
authors.initials: E
authors.lastname: Martín
authors.forename: D
authors.initials: D
authors.lastname: Hernández
authors.forename: R
authors.initials: R
authors.lastname: Sánchez-Monge
authors.forename: D
authors.initials: D
authors.lastname: Barber
authors.forename: V
authors.initials: V
authors.lastname: del Pozo
authors.forename: B
authors.initials: B
authors.lastname: de Andrés
authors.forename: A
authors.initials: A
authors.lastname: Armentia
authors.forename: C
authors.initials: C
authors.lastname: Lahoz
authors.forename: G
authors.initials: G
authors.lastname: Salcedo
mesh_terms: D000485:Allergens / Q000032:analysis* / Q000276:immunology; D000941:Antigens / Q000032:analysis* / Q000276:immunology; D001249:Asthma / Q000276:immunology*; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology; D008970:Molecular Weight; D014908:Triticum / Q000276:immunology*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*
chemical_list: D000485:Allergens; D000941:Antigens; D007106:Immune Sera; D007073:Immunoglobulin E; D000516:alpha-Amylases