A one step cell sorting procedure for the enrichment of murine bone marrow CFU-S which is independent of their proliferative activity.
Murine bone marrow day 9 splenic colony-forming units (CFU-S) have been concentrated using a one step cell sorting technique. CFU-S were discriminated on the basis of their rather high forward light scatter intensity, their high affinity for the lectin WGA and the absence of a cell surface marker expressed by the majority of hematopoietic cells and recognized by the RA3-5B3 MoAb. This procedure permitted a 40-fold enrichment of quiescent or cycling CFU-S (obtained from normal and Ara-C-treated mice respectively) and did not alter their differentiation pathways towards the various myeloid lineages.
Lepault
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Cellular Kinetics Research Unit, INSERM U250-Gustave Roussy Institute, Villejuif, France.
Fache
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MP
Frindel
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D001853:Bone Marrow / Q000378:metabolism; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002469:Cell Separation / Q000379:methods*; D003561:Cytarabine / Q000494:pharmacology; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D008027:Light; D051379:Mice; D008808:Mice, Inbred CBA; D012542:Scattering, Radiation; D013154:Spleen / Q000166:cytology; D014909:Wheat Germ Agglutinins / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D014909:Wheat Germ Agglutinins; D003561:Cytarabine