Immune function in transplanted small intestine. Total secretory IgA production and response against cholera toxin.
Secretory IgA is the dominant immunoglobulin produced in the small intestine and one important component of the local defense against dietary and infectious agents present in the gut lumen. The effect of small intestine transplantation on total production of sIgA and on the response to a newly presented antigen, cholera toxin, was determined in a rat segmental heterotopic intestinal transplant model. Lewis x Brown Norway F1 (LBNF1) allografts in Lewis hosts made normal amounts of sIgA, when compared with LBNF1 Thiry-Vella loops or LBNF1 isografts. In contrast, the allografts failed to make a significant specific sIgA response when immunized with cholera toxin at days 0 and 7 following transplantation. This failure was not the result of surgical manipulation, as isografts made normal amounts of specific sIgA directed against cholera toxin. Cyclosporine immunosuppression delayed, but did not prevent, the secretion of specific antibody in isografts. This failure to respond to a new antigen may have important implications for the safety of small bowel transplantation.
Xia
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Department of Surgery, Brigham and Women's Hospital, Boston, MA 02115.
Kirkman
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RL
D000818:Animals; D002772:Cholera Toxin / Q000276:immunology*; D003524:Cyclosporins / Q000494:pharmacology; D007114:Immunization; D007071:Immunoglobulin A, Secretory / Q000096:biosynthesis*; D007421:Intestine, Small / Q000276:immunology / Q000637:transplantation*; D051381:Rats; D011919:Rats, Inbred Strains
D003524:Cyclosporins; D007071:Immunoglobulin A, Secretory; D002772:Cholera Toxin