Arylalkylamine (serotonin) N-acetyltransferase assay using high-performance liquid chromatography with fluorescence or electrochemical detection of N-acetyltryptamine.
A sensitive, rapid, and economical method has been developed for determination of serotonin N-acetyltransferase activity from a variety of enzyme sources. The assay is based upon separation and detection of N-acetyltryptamine formed from tryptamine and acetyl coenzyme A, by means of high-performance liquid chromatography with either electrochemical or fluorometric detection. The limit of sensitivity with both detection methods is less than 20 pmol of N-acetyltryptamine formed per sample. A method for synthesis of N-acetyltryptamine, used as an external standard in the assay, is described.
Thomas
K B
KB
Emory University School of Medicine, Department of Pharmacology, Atlanta, Georgia 30322.
Zawilska
J
J
Iuvone
P M
PM
D000123:Acetyltransferases / Q000032:analysis*; D000818:Animals; D001191:Arylamine N-Acetyltransferase / Q000032:analysis*; D002642:Chick Embryo; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D004563:Electrochemistry; D005453:Fluorescence; D008297:Male; D010870:Pineal Gland / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D012160:Retina / Q000201:enzymology; D014363:Tryptamines / Q000032:analysis*
D014363:Tryptamines; C043464:N-acetyltryptamine; D000123:Acetyltransferases; D001191:Arylamine N-Acetyltransferase