title: Effect of specific amino acid groups on renal hemodynamics in humans.
abstract: The effect of specific amino acid groups on renal hemodynamics was examined in seven healthy young volunteers. Each subject received a 3-h intravenous infusion according to one of the following protocols: study 1, gluconeogenic amino acids (Arg, Gly, Pro, Cys, Met, Ser); study 2, alanine alone; study 3, branched-chain amino acids (BCAA, Leu, Ile, Val); or study 4, 0.9% saline. The rise (40-60% above base line) in total plasma amino acid concentration was similar in studies 1-3; no change was observed in study 4. During study 1, glomerular filtration rate (GFR) rose by 16% (from 98 +/- 6 to 114 +/- 8 ml.1.73 m-2.min-1, P less than 0.01), and renal plasma flow (RPF) rose by 28% (from 496 +/- 47 to 638 +/- 70 ml.1.73 m-2.min-1, P less than 0.01). After alanine (study 2) and BCAA (study 3) infusion, there was a slight, although not significant, rise in GFR and RPF; during saline infusion (study 4), GFR and RPF remained unchanged. Plasma insulin and growth hormone did not change significantly in any study protocol. Plasma glucagon rose significantly by 30% in study 1 (from 117 +/- 10 to 151 +/- 13 pg/ml, P less than 0.05) but did not change in studies 2-4. In summary, infusion of mixed gluconeogenic amino acids increases both GFR and RPF, and neither alanine nor BCAA infusion caused a consistent alteration in renal hemodynamics.
authors.affiliation: University of Texas Health Science Center, San Antonio 78284.
authors.forename: P
authors.initials: P
authors.lastname: Castellino
authors.forename: R
authors.initials: R
authors.lastname: Levin
authors.forename: J
authors.initials: J
authors.lastname: Shohat
authors.forename: R A
authors.initials: RA
authors.lastname: DeFronzo
mesh_terms: D000328:Adult; D000596:Amino Acids / Q000097:blood / Q000494:pharmacology*; D001786:Blood Glucose / Q000032:analysis; D001794:Blood Pressure; D005919:Glomerular Filtration Rate; D005934:Glucagon / Q000097:blood; D013006:Growth Hormone / Q000097:blood; D006439:Hemodynamics / Q000187:drug effects; D006801:Humans; D008297:Male; D011674:Pulse; D012079:Renal Circulation / Q000187:drug effects
chemical_list: D000596:Amino Acids; D001786:Blood Glucose; D013006:Growth Hormone; D005934:Glucagon