Preparation and characterization of polyclonal and monoclonal antibodies against the insecticide DDT.
A synthetic DDT derivative in which the molecular structure of DDT was completely retained was coupled to bovine serum albumin. Animals were immunized with the DDT-bovine serum albumin conjugate and polyclonal and monoclonal antibodies against the insecticide were isolated. These antibodies seemed to be the first true anti-DDT antibodies and distinguished much better between DDT and DDT metabolites than previously prepared anti-DDT antisera. In competitive solid phase radioimmunoassays, DDT concentrations as low as 10 nM or 0.0035 mg/1 were detectable. The anti-DDT antibodies can be used for environmental analyses and lend themselves to the elucidation of the structure of the DDT binding site.
Bürgisser
D
D
Biochemisches Institut der Universität Zürich, Switzerland.
Frey
S
S
Gutte
B
B
Klauser
S
S
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D003634:DDT / Q000032:analysis / Q000276:immunology*; D005260:Female; D006241:Haptens; D007202:Indicators and Reagents; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008970:Molecular Weight; D009113:Muramidase; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D012710:Serum Albumin, Bovine
D000906:Antibodies; D000911:Antibodies, Monoclonal; D006241:Haptens; D007202:Indicators and Reagents; D012710:Serum Albumin, Bovine; D003634:DDT; D009113:Muramidase