title: Prostaglandin D2 modulates human neutrophil intracellular calcium flux and inhibits superoxide release via its ring carbonyl.
abstract: We compared the effects of prostaglandin D2 (PGD2), prostaglandin F2 alpha (PGF2) and various ketones on superoxide (OX) release by human neutrophils, which had been stimulated by N-formyl methionyl leucyl phenylalanine (FMLP). Our data suggested that the ring carbonyl of PGD2 is essential to its inhibitory effect on OX release, but the carbonyl group as a ketone, alone is not sufficient. Using the fluorescent Ca2+ probe, Fura-2AM, we found that PGD2 increased the rate of decline of FMLP stimulated intracellular free Ca2+ (Ca)i, but that PGF2 had no effect. cAMP altered FMLP stimulated (Ca)i, in a pattern similar to PGD2. Furthermore, the ring carbonyl of PGD2 is crucial to its effect on OX as well as on (Ca)i.
authors.affiliation: Naval Medical Research Institute, Bethesda, Maryland 20814-5055.
authors.forename: C O
authors.initials: CO
authors.lastname: Simpkins
authors.forename: D L
authors.initials: DL
authors.lastname: Mazorow
authors.forename: S T
authors.initials: ST
authors.lastname: Alailima
authors.forename: E A
authors.initials: EA
authors.lastname: Tate
authors.forename: W
authors.initials: W
authors.lastname: Sweatt
authors.forename: M
authors.initials: M
authors.lastname: Johnson
authors.forename: K
authors.initials: K
authors.lastname: Shariff
authors.forename: D B
authors.initials: DB
authors.lastname: Millar
mesh_terms: D002118:Calcium / Q000378:metabolism*; D006801:Humans; D007659:Ketones / Q000494:pharmacology; D007700:Kinetics; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D015230:Prostaglandin D2 / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D007659:Ketones; D009240:N-Formylmethionine Leucyl-Phenylalanine; D013482:Superoxide Dismutase; D015230:Prostaglandin D2; D002118:Calcium