Rapid technique for cryopreservation of Echinococcus multilocularis metacestodes.
Cysts of E. multilocularis were minced to prepare a crude homogenate and after addition of glycerol at a final concentration of 10%, cryopreservation was performed at a rate of 1 degree C min-1 in a controlled-rate freezer. The aliquots were subsequently stored in liquid nitrogen. All 22 isolates tested were successfully cryopreserved and their viability maintained.
Bretagne
S
S
Laboratoire de Parasitologie, Faculté de Médecine, Creteil, France.
Beaujean
F
F
Uner
A
A
Bresson-Hadni
S
S
Liance
M
M
Houin
R
R
D000818:Animals; D015925:Cryopreservation; D004446:Echinococcus / Q000254:growth & development*