title: Reduction of complement activation during bypass by prime manipulation.
abstract: Complement activation is believed to be of importance in the development of complications arising after cardiopulmonary bypass. The effect on complement activation of priming the extracorporeal circuit with crystalloid alone, crystalloid plus albumin, or crystalloid plus the plasma expander polygeline was assessed in 36 patients undergoing coronary artery operations with cardiopulmonary bypass using a bubble oxygenator. Activation of the alternative and common complement pathways was monitored before, during, and after the bypass period by measuring concentrations of factor B and its fragment Ba and C3 and its fragment C3d. Complement activation occurred in all three groups of patients, with no difference between the crystalloid and crystalloid-albumin groups. In contrast, Ba fragment concentrations were persistently and significantly lower during and after bypass in the polygeline group, denoting reduced complement activation. C3d levels also showed a tendency to be lower in this group. Our results indicate that addition of polygeline to the priming solution reduces complement activation. Because complement activation is associated with morbidity after cardiopulmonary bypass, addition of polygeline to the priming solution may offer an inexpensive method of reducing morbidity after cardiopulmonary bypass.
authors.affiliation: Department of Surgery, Brompton Hospital, London, England.
authors.forename: R S
authors.initials: RS
authors.lastname: Bonser
authors.forename: J R
authors.initials: JR
authors.lastname: Dave
authors.forename: E T
authors.initials: ET
authors.lastname: Davies
authors.forename: L
authors.initials: L
authors.lastname: John
authors.forename: P
authors.initials: P
authors.lastname: Taylor
authors.forename: H
authors.initials: H
authors.lastname: Gaya
authors.forename: S C
authors.initials: SC
authors.lastname: Lennox
authors.forename: D
authors.initials: D
authors.lastname: Vergani
mesh_terms: D000418:Albumins / Q000008:administration & dosage / Q000494:pharmacology*; D002315:Cardiopulmonary Bypass / Q000379:methods; D003167:Complement Activation / Q000187:drug effects*; D003176:Complement C3 / Q000187:drug effects; D003179:Complement C3b / Q000032:analysis; D015933:Complement C3d / Q000187:drug effects; D011415:Complement Factor B / Q000032:analysis; D005260:Female; D006801:Humans; D007552:Isotonic Solutions / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000032:analysis; D011097:Polygeline / Q000008:administration & dosage / Q000494:pharmacology*; D011108:Polymers / Q000494:pharmacology*; D011479:Protamines / Q000494:pharmacology; D011897:Random Allocation; D000077325:Ringer's Lactate
chemical_list: D000418:Albumins; D003176:Complement C3; D007552:Isotonic Solutions; D010446:Peptide Fragments; D011108:Polymers; D011479:Protamines; D000077325:Ringer's Lactate; C051367:complement C3bBa complex; D003179:Complement C3b; D015933:Complement C3d; D011097:Polygeline; D011415:Complement Factor B