Expression of cellCAM-105 in the apical surface of rat uterine epithelium is controlled by ovarian steroid hormones.
Affinity-purified antibodies to cellCAM-105, an adhesive cell surface glycoprotein, were used in immunohistochemical investigations of rat uteri at various functional stages: (i) the oestrous, pro-oestrous, metoestrous, and dioestrous stages of the oestrous cycle, (ii) Days 1-8 of normal pregnancy, (iii) delayed implantation, (iv) 18 h after oestrogen reactivation from delay of implantation, and (v) juvenile rats, and normal ovariectomized adults, respectively, before and after experimental injection of progesterone and/or oestrogen. CellCAM-105 was present in the apical zones of the luminal and glandular epithelium cells in a stage-specific and hormone-dependent manner. The results indicate that: (1) steroid hormones are essential for the expression of cellCAM-105 in the uterine epithelial cells; (2) progesterone induces cellCAM-105 expression in the glandular epithelium, and oestrogen induces cellCAM-105 expression in the luminal epithelium; (3) progesterone induces down-regulation of cellCAM-105 from the surface of the uterine luminal epithelium of juvenile rats; (4) cellCAM-105 is absent in the luminal epithelial cells but present in the glandular epithelial cells of the rat uterus at the time of blastocyst implantation.
Svalander
P C
PC
Department of Human Anatomy, Uppsala University, Sweden.
Odin
P
P
Nilsson
B O
BO
Obrink
B
B
D000251:Adenosine Triphosphatases; D000818:Animals; D015703:Antigens, CD; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D010065:Embryo Implantation, Delayed / Q000502:physiology; D004848:Epithelium / Q000378:metabolism; D004967:Estrogens / Q000494:pharmacology; D004971:Estrus / Q000378:metabolism; D005260:Female; D012739:Gonadal Steroid Hormones / Q000378:metabolism*; D008562:Membrane Glycoproteins; D010052:Ovariectomy; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D014599:Uterus / Q000378:metabolism*
D015703:Antigens, CD; C069847:CD66 antigens; D015815:Cell Adhesion Molecules; D004967:Estrogens; D012739:Gonadal Steroid Hormones; D008562:Membrane Glycoproteins; D011374:Progesterone; D000251:Adenosine Triphosphatases