Teichuronic acid reducing terminal N-acetylglucosamine residue linked by phosphodiester to peptidoglycan of Micrococcus luteus.
Teichuronic acid-peptidoglycan complex isolated from Micrococcus luteus cells by lysozyme digestion in osmotically stabilized medium was treated with mild acid to cleave the linkage joining teichuronic acid to peptidoglycan. This labile linkage was shown to be the phosphodiester which joins N-acetylglucosamine, the residue located at the reducing end of the teichuronic acid, through its anomeric hydroxyl group to a 6-phosphomuramic acid, a residue of the glycan strand of peptidoglycan. 31P nuclear magnetic resonance spectroscopy of the lysozyme digest of cell walls demonstrated the presence of a phosphodiester which was converted to a phosphomonoester by the conditions which released teichuronic acid from cell walls. Reduction of acid-liberated reducing end groups by NaB3H4 followed by complete acid hydrolysis yielded [3H] glucosaminitol from the true reducing end residue of teichuronic acid and [3H]glucitol from the sites of fragmentation of teichuronic acid. The amount of N-acetylglucosamine detected was approximately stoichiometric with the amount of phosphate in the complex. Partial fragmentation of teichuronic acid provides an explanation of the previous erroneous identification of the reducing end residue.
Gassner
G T
GT
Department of Biochemistry, College of Biological Sciences, University of Minnesota, St. Paul 55108.
Dickie
J P
JP
Hamerski
D A
DA
Magnuson
J K
JK
Anderson
J S
JS
D000117:Acetylglucosamine / Q000032:analysis*; D001894:Borohydrides; D002458:Cell Fractionation; D002473:Cell Wall / Q000032:analysis / Q000648:ultrastructure; D005944:Glucosamine / Q000031:analogs & derivatives* / Q000032:analysis; D006868:Hydrolysis; D008837:Micrococcus / Q000032:analysis*; D009113:Muramidase; D010457:Peptidoglycan / Q000302:isolation & purification*; D010710:Phosphates / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification*; D014574:Uronic Acids / Q000302:isolation & purification*
D001894:Borohydrides; D010457:Peptidoglycan; D010710:Phosphates; D011135:Polysaccharides, Bacterial; D014574:Uronic Acids; C010788:teichuronic acid; D009113:Muramidase; D005944:Glucosamine; D000117:Acetylglucosamine