In vivo analysis of extracellular proteins in rat brains with a newly developed intracerebral microdialysis probe.
Peptides and proteins in the extracellular space in the central nervous system were investigated in vivo using an intracerebral microdialysis probe. The molecular cut-off of the hollow fiber which was used for the probe was approximately 100 kDa. We examined recovery rates of several compounds in vitro. The recovery rates of proteins and peptides were between 7-28%, with the exceptions of substance P and insulin-like growth factor I. The recovery rates of monoamines and their metabolites were 22-40%. In in vivo studies, two major proteins with apparent molecular weights of 62 kDa and 12 kDa, and several minor proteins (28 kDa, 43 kDa, 52 kDa and 70 kDa) were detected by SDS-polyacrylamide gel electrophoresis in the dialysate from a probe implanted in the striatum of anesthetized rats. These results suggest that the newly developed, intracerebral microdialysis probe might be useful for investigating the dynamic changes of peptides and proteins in the central nervous system.
Nakamura
M
M
Department of Neuropsychiatry, Kagawa Medical School, Japan.
Itano
T
T
Yamaguchi
F
F
Mizobuchi
M
M
Tokuda
M
M
Matsui
H
H
Etoh
S
S
Hosokawa
K
K
Ohmoto
T
T
Hatase
O
O
D000818:Animals; D001923:Brain Chemistry; D003956:Dialysis / Q000295:instrumentation; D004591:Electrophoresis, Polyacrylamide Gel; D004867:Equipment Design; D005110:Extracellular Space / Q000032:analysis*; D010455:Peptides / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D010455:Peptides; D011506:Proteins