Host cell proteins required for measles virus reproduction.
We have developed a cell-free system derived from measles virus-infected cells that supported the transcription and replication of measles virus RNA in vitro. The data suggest that tubulin may be required for these reactions, since an anti-beta-tubulin monoclonal antibody inhibited viral RNA synthesis and the addition of purified tubulin stimulated measles virus RNA synthesis in vitro. Tubulin may be a subunit of the viral RNA polymerase, since two different anti-tubulin antibodies, one specific for the beta- and another specific for the alpha-subunit of tubulin, coimmunoprecipitated the measles virus L protein as well as tubulin from extracts of measles virus-infected cells. Other experiments further implicated actin in the budding process during virus maturation, as there appeared to be a specific association of actin in vitro only with nucleocapsids that have terminated RNA synthesis, which is presumably a prerequisite to budding.
Moyer
S A
SA
Department of Immunology and Medical Microbiology, University of Florida College of Medicine, Gainesville 32610.
Baker
S C
SC
Horikami
S M
SM
D000199:Actins / Q000502:physiology*; D000818:Animals; D002213:Capsid / Q000096:biosynthesis; D002460:Cell Line; D002642:Chick Embryo; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007700:Kinetics; D008459:Measles virus / Q000502:physiology*; D011233:Precipitin Tests; D012367:RNA, Viral / Q000096:biosynthesis*; D014158:Transcription, Genetic; D014404:Tubulin / Q000502:physiology*; D014758:Viral Core Proteins / Q000096:biosynthesis; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
D000199:Actins; D012367:RNA, Viral; D014404:Tubulin; D014758:Viral Core Proteins; D014764:Viral Proteins