Mini-mouse: disruption of the pygmy locus in a transgenic insertional mutant.
A founder transgenic mouse harbored two different integration patterns of a transgene at the same locus, each of which gave rise to a similar autosomal recessive mutation. Mice of the mutant phenotype were of small stature but had normal levels of growth hormone. The disrupted locus was cloned, and a genetic and molecular analysis showed that the insertional mutants were allelic to a spontaneous mutant, pygmy. The mice should be a useful model for the growth hormone-resistant human dwarf syndromes and could lead to a greater understanding of the pathways involved in growth and development.
Xiang
X
X
Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854.
Benson
K F
KF
Chada
K
K
D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004195:Disease Models, Animal; D004392:Dwarfism / Q000235:genetics*; D005260:Female; D013006:Growth Hormone / Q000097:blood; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D008822:Mice, Transgenic; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010375:Pedigree; D015183:Restriction Mapping
D013006:Growth Hormone; D004247:DNA