Persistent enterovirus infection in culture-negative meningoencephalitis: demonstration by enzymatic RNA amplification.
Chronic meningoencephalitis due to enterovirus infection can occur in patients with antibody deficiencies. A modified polymerase chain reaction technique demonstrated persistent echovirus 11 infection in such a patient, despite negative routine viral cultures and negative routine nucleic acid hybridization. Although the sequence of echovirus 11 has not yet been determined, genomic conservation among the enteroviruses is significant, permitting detection of echovirus 11 with a primer pair and probe derived from enterovirus serotypes that have been fully sequenced. This study provides the first definitive evidence for the persistence of enterovirus infection with negative viral cultures.
Rotbart
H A
HA
Department of Pediatrics, University of Colorado School of Medicine, Denver 80262.
Kinsella
J P
JP
Wasserman
R L
RL
D000361:Agammaglobulinemia / Q000150:complications; D001483:Base Sequence; D002648:Child; D004457:Echovirus Infections / Q000134:cerebrospinal fluid / Q000150:complications / Q000175:diagnosis*; D004587:Electrophoresis, Agar Gel; D029822:Enterovirus B, Human / Q000235:genetics* / Q000302:isolation & purification; D005784:Gene Amplification; D006801:Humans; D008297:Male; D008590:Meningoencephalitis / Q000134:cerebrospinal fluid / Q000150:complications / Q000175:diagnosis*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics
D012367:RNA, Viral