title: The inhibitory influence of tracheal mucosa mounted in close proximity to canine trachealis.
abstract: Reduced smooth muscle contractile responses to agonists occur in the presence of epithelium, perhaps due to the release of an epithelium-derived relaxing factor (EpDRF). It is not clear whether the release of EpDRF requires the direct attachment of the epithelium to the smooth muscle. In the present study, using isolated canine tracheal smooth muscle strips, we examined whether the inhibitory effects of airway mucosa require the attachment of the mucosa to smooth muscle. The smooth muscle contractile responses to acetylcholine and histamine were reduced in the presence of airway mucosa, whether the mucosa was attached or in close proximity. The inhibitory effect mediated by the airway mucosa therefore is not dependent on mucosal attachment to smooth muscle. This phenomenon appears to be due to the release of a soluble, short-acting mediator from the airway mucosa.
authors.affiliation: Department of Medicine, McMaster University, Hamilton, Ontario, Canada.
authors.forename: P J
authors.initials: PJ
authors.lastname: Manning
authors.forename: G L
authors.initials: GL
authors.lastname: Jones
authors.forename: J
authors.initials: J
authors.lastname: Otis
authors.forename: E E
authors.initials: EE
authors.lastname: Daniel
authors.forename: P M
authors.initials: PM
authors.lastname: O'Byrne
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001685:Biological Factors / Q000502:physiology*; D004285:Dogs; D004848:Epithelium / Q000502:physiology; D006632:Histamine / Q000031:analogs & derivatives / Q000494:pharmacology; D066298:In Vitro Techniques; D009092:Mucous Membrane / Q000378:metabolism; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000502:physiology*; D014132:Trachea / Q000502:physiology*
chemical_list: D001685:Biological Factors; C056250:epithelium-derived relaxant factor; D006632:Histamine; D000109:Acetylcholine; C027556:histamine phosphate