title: Electroimmunoblotting of neuropeptide Y: application to the rat vas deferens.
abstract: In this study we have optimized the electroimmunoblotting conditions for neuropeptide Y (NPY). NPY standards and samples extracted from the rat vas deferens were separated on urea-sodium dodecyl sulphate gels. Densitometric scanning of the Coomassie Blue-stained gels allow a semi-quantitative analysis of NPY in the range of approximately 10(-11) to 10(-8) mol of NPY. Electroimmunoblotting of NPY was also shown to be best achieved overnight at 4 degrees C and with NC membranes of 0.22 micron. Under these conditions NPY extracted from the vas deferens has been efficiently electroimmunoblotted. Higher molecular weight NPY-reactive peptides were also detected that may be related to proteolytic processing of the NPY precursor.
authors.affiliation: Department of Pharmacology, Universidad Central del Caribe, School of Medicine, Cayey, PR 00634.
authors.forename: W I
authors.initials: WI
authors.lastname: Silva
authors.forename: N
authors.initials: N
authors.lastname: Santiago
authors.forename: J E
authors.initials: JE
authors.lastname: García-Arrarás
authors.forename: B
authors.initials: B
authors.lastname: Carrasquillo
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods*; D008297:Male; D009478:Neuropeptide Y / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D014649:Vas Deferens / Q000378:metabolism*
chemical_list: D009478:Neuropeptide Y