A satellite DNA family from pollock (Pollachius virens).
We have cloned and sequenced a highly reiterated EcoRI fragment of DNA from pollock (Pollachus virens). The EcoRI repeat is 200 (+/- 5) bp long, A + T-rich (65%) and exhibits a high degree of sequence conservation among representative members. It comprises 13% of the pollock nuclear DNA with a copy number of 5 x 10(5) per haploid genome. Partial digestion of pollock DNA with EcoRI or Hinf1, which cleaves within the repeat, followed by blot hybridization to a cloned repeat sequence, produced a ladder of hybridising bands indicating a tandemly arrayed organisation for the EcoRI repeat. Longer range periodicities, revealed by restriction endonuclease digestion, are superimposed on the tandem array.
Denovan
E M
EM
Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada.
Wright
J M
JM
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004276:DNA, Satellite / Q000235:genetics*; D015246:Deoxyribonuclease EcoRI; D005399:Fishes / Q000235:genetics*; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid
D004276:DNA, Satellite; D015246:Deoxyribonuclease EcoRI