A new inhibitor of protein kinase C, RK-286C (4'-demethylamino-4'-hydroxystaurosporine). II. Isolation, physico-chemical properties and structure.
RK-286C, a new inhibitor of protein kinase C, has been found by the bleb-forming assay using K562 cells. It was produced by Streptomyces sp. RK-286 and purified by solvent extraction, silica gel chromatography and preparative HPLC. Spectrometric analysis revealed that the structure is 4'-demethylamino-4'-hydroxystaurosporine.
Takahashi
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Antibiotics Laboratory, RIKEN, Institute of Physical and Chemical Research, Saitama, Japan.
Osada
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H
Uramoto
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Isono
K
K
D000470:Alkaloids / Q000032:analysis / Q000302:isolation & purification*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D013053:Spectrophotometry; D019311:Staurosporine / Q000031:analogs & derivatives; D013302:Streptomyces / Q000032:analysis
D000470:Alkaloids; D003470:Culture Media; C063438:4'-demethylamino-4'-hydroxystaurosporine; D011493:Protein Kinase C; D019311:Staurosporine