A monoclonal anti-imipramine antibody with antidepressant binding properties similar to the muscarinic receptor.
A murine monoclonal antibody raised using imipramine conjugated to bovine serum albumin bound free imipramine with high affinity (KD = 24 nM). The antibody had a binding affinity profile for tricyclic and other uptake-inhibitor type antidepressants which correlated highly with the affinities of the same compounds for the brain acetylcholine muscarinic receptor, but not for plasma-membrane 5-hydroxytryptamine carriers. This antibody was not similar in binding properties to polyclonal antisera produced using the same antigen but was highly similar in binding properties to a monoclonal anti-nortriptyline antibody, ANT3, raised by Marulo et al. (Marulo S, Hoebeke J, Guillet JG, Andre C and Strosberg AD, J Immunol 138: 524-526, 1987) using a different antigen.
Ronayne
L
L
Department of Biochemistry, Trinity College, Dublin, Ireland.
McInerney
M
M
Phillips
O M
OM
Regan
C M
CM
Williams
D C
DC
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D000915:Antibody Affinity; D000928:Antidepressive Agents / Q000276:immunology*; D000941:Antigens / Q000276:immunology; D001666:Binding Sites, Antibody; D006241:Haptens; D007099:Imipramine / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011976:Receptors, Muscarinic / Q000276:immunology*; D012710:Serum Albumin, Bovine / Q000276:immunology
D000911:Antibodies, Monoclonal; D000928:Antidepressive Agents; D000941:Antigens; D006241:Haptens; D011976:Receptors, Muscarinic; D012710:Serum Albumin, Bovine; D007099:Imipramine