Identification of the monocyte chemotactic protein from human osteosarcoma cells and monocytes: detection of a novel N-terminally processed form.
The chemotactic activity for monocytes in culture supernatants from double-stranded RNA-stimulated human MG-63 osteosarcoma cells and from LPS-stimulated human monocytes was purified to homogeneity and characterized by amino acid sequence analysis. The chemotactic protein derived from the fibroblastoid osteosarcoma cells had a blocked N-terminus but sequencing of tryptic fragments showed that it was identical with a recently identified monocyte chemoattractant designated MCP-1 or MCAF isolated from glioma or myelomonocytic cells, respectively. Preparations of monocyte -derived chemotactic activity appeared to contain not only the blocked protein, but also a novel N-terminally processed form of this molecule, lacking 5 amino acid residues.
Decock
B
B
Rega Institute for Medical Research, University of Leuven, Belgium.
Conings
R
R
Lenaerts
J P
JP
Billiau
A
A
Van Damme
J
J
D000595:Amino Acid Sequence; D002460:Cell Line; D018932:Chemokine CCL2; D002630:Chemotactic Factors / Q000097:blood / Q000302:isolation & purification* / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology; D008969:Molecular Sequence Data; D009000:Monocytes / Q000032:analysis* / Q000166:cytology / Q000187:drug effects; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D012516:Osteosarcoma / Q000032:analysis*; D010446:Peptide Fragments / Q000302:isolation & purification; D014407:Tumor Cells, Cultured / Q000032:analysis
D018932:Chemokine CCL2; D002630:Chemotactic Factors; D008070:Lipopolysaccharides; D010446:Peptide Fragments; D009240:N-Formylmethionine Leucyl-Phenylalanine