Immunoassay by particle counting for coagulation testing: application to the determination of protein C.
Latex particles coated with F(ab)'2 fragments of anti-protein C IgG antibodies are agglutinated by protein C, and the quantity of particles agglutinated is proportional to the concentration of protein C. The reaction can be quantitated by optical particle counting. Based on this system, we designed an immunoassay for protein C. Precision measured at low, medium and high levels of protein C varied from 3.3% to 13.7%. Specificity was evaluated by dilution recovery. A correlation coefficient of r = 0.959 was found when the new method was compared with a chromogenic technique on 131 plasmas.
Poncelet
S
S
Unit of Experimental Medicine, Faculty of Medicine, Catholic University of Louvain.
Lavenne
E
E
Limet
J N
JN
Collet-Cassart
D
D
D000818:Animals; D001780:Blood Coagulation Tests / Q000379:methods*; D002138:Calibration; D002417:Cattle; D002863:Chromogenic Compounds / Q000378:metabolism; D006801:Humans; D007118:Immunoassay / Q000379:methods; D011486:Protein C / Q000032:analysis
D002863:Chromogenic Compounds; D011486:Protein C