title: Secondary structure of the human membrane-associated folate binding protein using a joint prediction approach.
abstract: The secondary structures of the human membrane-associated folate binding protein (FBP) and bovine soluble FBP are assessed by a joint prediction approach that combines neural network models, information theory, homology modeling and the Chou-Fasman methods. Two new profile maps are used to characterize the non-regular secondary structure and to assist in assigning buried and exposed parts of secondary structure: (i) the loop potential profile and (ii) the long range contact profile. Approximately half of human FBP is predicted to form regular secondary structure (alpha-helices-35% or beta-sheets - 12%, excluding the transmembrane helices) and the rest is predicted to form coil, turns or loops. The bovine milk soluble FBP is predicted to have a similar secondary structure as expected because of the high degree of homology between the FBP's. Discriminant analysis predicts two transmembrane segments for the human FBP sequence, one at the amino terminus (a leader sequence) and the other at the carboxy terminus. These predicted transmembrane domains are absent in the bovine milk soluble FBP, further supporting these predictions. The present set of secondary structural predictions for human FBP is obtained by 'consensus' to aid in modeling the super-secondary structure of the protein.
authors.affiliation: Theoretical Immunology Section, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
authors.forename: V N
authors.initials: VN
authors.lastname: Viswanadhan
authors.forename: J N
authors.initials: JN
authors.lastname: Weinstein
authors.forename: P C
authors.initials: PC
authors.lastname: Elwood
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins; D002417:Cattle; D058973:Folate Receptors, GPI-Anchored; D006801:Humans; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011956:Receptors, Cell Surface; D012689:Sequence Homology, Nucleic Acid; D013223:Statistics as Topic
chemical_list: D002352:Carrier Proteins; D058973:Folate Receptors, GPI-Anchored; D008565:Membrane Proteins; D011956:Receptors, Cell Surface