Assessment of vascular permeability increase in the mouse by dye leakage during paw edema.
Vascular permeability increase induced by histamine, bradykinin, platelet-activating factor (PAF), or phorbol myristate acetate (PMA) in the mouse paw was assessed by the dye leakage method. The amount of dye extracted from the paw showed a clear dose-response relationship to the dose of each agonist injected into the paw. Among the autacoids used, PAF showed the most potent activity in the mouse paw. The results are consistent with those seen in the rat dorsal skin as previously reported. Involvement of histamine, 5-hydroxytryptamine and PAF is suggested in the vascular permeability increase induced by PMA in the mouse paw.
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Department of Pharmacology, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
Hayashi
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Yamaki
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Oh-ishi
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D000818:Animals; D001920:Bradykinin / Q000494:pharmacology; D002199:Capillary Permeability / Q000187:drug effects*; D004396:Coloring Agents; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000503:physiopathology*; D005528:Foot / Q000503:physiopathology; D006632:Histamine / Q000494:pharmacology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010742:Phospholipid Ethers / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000494:pharmacology; D011738:Pyrilamine / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D004396:Coloring Agents; D010742:Phospholipid Ethers; D010972:Platelet Activating Factor; D006632:Histamine; C037913:CV 3988; D011738:Pyrilamine; D013755:Tetradecanoylphorbol Acetate; D001920:Bradykinin