Fatty acylation promotes fusion of transport vesicles with Golgi cisternae.
Two different methods, stimulation of transport by fatty acyl-coenzyme A (CoA) and inhibition of transport by a nonhydrolyzable analogue of palmitoyl-CoA, reveal that fatty acylation is required to promote fusion of transport vesicles with Golgi cisternae. Specifically, fatty acyl-CoA is needed after the attachment of coated vesicles and subsequent uncoating of the vesicles, and after the binding of the NEM-sensitive fusion protein (NSF) to the membranes, but before the actual fusion event. We therefore suggest that an acylated transport component participates, directly or indirectly, in membrane fusion.
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Department of Biology, Princeton University, New Jersey 08544-1014.
Glick
B S
BS
Arden
S R
SR
Rothman
J E
JE
D000214:Acyl Coenzyme A / Q000494:pharmacology*; D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism*; D002474:Cell-Free System; D003065:Coenzyme A / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology; D006056:Golgi Apparatus / Q000187:drug effects / Q000378:metabolism*; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008561:Membrane Fusion / Q000187:drug effects*; D052066:N-Ethylmaleimide-Sensitive Proteins; D017830:Octoxynol; D015388:Organelles / Q000187:drug effects / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000494:pharmacology; D033921:Vesicular Transport Proteins
D000214:Acyl Coenzyme A; D002352:Carrier Proteins; D003902:Detergents; D033921:Vesicular Transport Proteins; D000431:Ethanol; D011092:Polyethylene Glycols; C032421:heptadecan-2-onyl-dethio-coenzyme A; D017830:Octoxynol; D052066:N-Ethylmaleimide-Sensitive Proteins; D003065:Coenzyme A