title: A comparative study of enzyme histochemical features in the gerbilline and human cholesteatoma.
abstract: Spontaneous and experimentally induced cholesteatoma in the Mongolian gerbil has been found to exhibit histopathological similarities to human aural cholesteatoma and has been suggested as an experimental model for studies of the clinical situation. In an attempt to further characterize this model, we compared experimentally induced cholesteatomas in the external auditory canal from gerbils with those of the human ear by means of a correlated histopathologic and enzyme histochemical study. The human and gerbilline cholesteatomas revealed similar histopathologic features. Even enzyme histochemically, the human and experimentally induced cholesteatomas demonstrated similar features. Thus glucose-6-phosphate dehydrogenase activity, an indicator of oxidative metabolism, was demonstrated especially in the stratum granulosum cells of the heavily orthokeratinizing squamous epithelium adjacent to the cholesteatomas. The human ear canal skin also revealed enzyme histochemical characteristics similar to the squamous epithelium lining the human cholesteatoma. The hydrolytic enzyme activity (leucyl-aminopeptidase) was strong in the connective tissue surrounding human cholesteatoma when compared with that of ear canal skin. In the gerbilline cholesteatoma, this activity was demonstrated especially in the connective tissue adjacent to eroded bone, which possibly may facilitate cholesteatoma progression. We conclude that experimentally induced cholesteatoma has both histophatological and enzyme histochemical similarities to human aural cholesteatoma and therefore it is suggested that the gerbilline model may be used for studies on the development of human cholesteatoma. Our results support the view that cholesteatoma may originate from migrated hyperkeratinizing cells from the epidermis of the tympanic membrane or the meatus.
authors.affiliation: Department of Otolaryngology, Sahlgrenska Sjukhuset, University of Göteborg, Sweden.
authors.forename: B
authors.initials: B
authors.lastname: Aberg
authors.forename: D
authors.initials: D
authors.lastname: Bagger-Sjöbäck
authors.forename: S
authors.initials: S
authors.lastname: Edström
authors.forename: G
authors.initials: G
authors.lastname: Heyden
authors.forename: M
authors.initials: M
authors.lastname: Jacobsson
authors.forename: L G
authors.initials: LG
authors.lastname: Kindblom
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000368:Aged; D000626:Aminopeptidases / Q000378:metabolism; D000818:Animals; D002781:Cholesteatoma / Q000201:enzymology* / Q000473:pathology; D004195:Disease Models, Animal; D004424:Ear Canal / Q000201:enzymology / Q000473:pathology; D004427:Ear Diseases / Q000201:enzymology* / Q000473:pathology; D004432:Ear, Middle / Q000201:enzymology / Q000473:pathology; D004848:Epithelium / Q000201:enzymology; D005260:Female; D005849:Gerbillinae; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: D005954:Glucosephosphate Dehydrogenase; D000135:Acid Phosphatase; D000626:Aminopeptidases