A cloned ATP:guanidino kinase in the trematode Schistosoma mansoni has a novel duplicated structure.
Creatine kinase (CK) is part of a conserved family of ATP:guanidino phosphotransferases whose members play important roles in intracellular energy flow. Previously characterized members of this family are approximately 80-kDa dimers of two related 40-kDa subunits. We have cloned a gene from the parasitic trematode Schistosoma mansoni which has substantial amino acid sequence similarities to CK. Like the genes for vertebrate CKs, this gene is developmentally regulated; mRNA levels are high in the infective cercarial stage but rapidly decrease upon transformation to the parasitic schistosomulum stage. In contrast to members of the guanidino phosphotransferase family characterized previously, however, the schistosome gene appears to be a direct fusion of two CK-like domains that encode a single 74-kDa polypeptide. Correlative evidence from enzyme assays of crude parasite homogenates suggests that the cloned gene is a creatine kinase. This represents the first molecular cloning of an invertebrate ATP:guanidino phosphotransferase.
Stein
L D
LD
Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts 02115.
Harn
D A
DA
David
J R
JR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003402:Creatine Kinase / Q000235:genetics*; D004247:DNA / Q000235:genetics; D015723:Gene Library; D051379:Mice; D008808:Mice, Inbred CBA; D008969:Molecular Sequence Data; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D011817:Rabbits; D015183:Restriction Mapping; D012156:Reticulocytes / Q000378:metabolism; D012550:Schistosoma mansoni / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D004247:DNA; D003402:Creatine Kinase