Morphiceptin analogs containing 2-aminocyclopentane carboxylic acid as a peptidomimetic for proline.
As part of a program to study the structure-activity relationship of peptide opioids we report the synthesis, conformational characterization and biological activity of four analogs related to morphiceptin in which the proline at position two has been substituted with 2-aminocyclopentane carboxylic acid (beta Ae5c). The beta Ac5c residue is a beta amino acid with two chiral centers resulting in four possible configurations; two configurational cis (R,S and S,R) and two configurational trans (R,R and S,S) forms. Utilizing high resolution n.m.r. at 500 MHz and computer simulations with NOE restraints the chirality of the beta Ac5c residues are assigned. The analog containing the R,S-beta Ac5c is active at both the mu and delta-opioid receptors, with a slight preference for the mu-receptor. The (S,R), (S,S), and (R,R) analogs show minimal activity at the mu-receptor and are inactive at the delta-receptor. A comparison of the findings from the conformational analysis and biological assays lends insight into the structure-activity relationship of this important peptide opiate.
Mierke
D F
DF
Department of Chemistry, University of California, San Diego, La Jolla.
Nössner
G
G
Schiller
P W
PW
Goodman
M
M
D000595:Amino Acid Sequence; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D003198:Computer Simulation; D003515:Cycloleucine / Q000031:analogs & derivatives; D004723:Endorphins / Q000096:biosynthesis; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008954:Models, Biological; D008969:Molecular Sequence Data; D011392:Proline; D011487:Protein Conformation; D013329:Structure-Activity Relationship
D004723:Endorphins; D003515:Cycloleucine; C065594:cispentacin; C028889:morphiceptin; D011392:Proline