title: A novel Fmoc-based anchorage for the synthesis of protected peptides on solid phase.
abstract: A novel bifunctional compound, 9-(hydroxymethyl)-2-fluoreneacetic acid, was synthesized, coupled to benzhydrylamine-resin, and evaluated for its application to the solid phase synthesis of protected peptide fragments. Anchor-bond cleavage was achieved with 15% piperidine/DMF. A protected heptapeptide, Boc-Val-Val-Ser(Bzl)-His(Tos)-Phe-Asn-Lys-(Z)-OH, corresponding to the sequence (1-7) of rat-transforming growth factor-alpha, was synthesized using this new support with an overall yield of 46%.
authors.affiliation: Shanghai Institute of Organic Chemistry, Academia Sinica, China.
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Liu
authors.forename: S H
authors.initials: SH
authors.lastname: Ding
authors.forename: J Y
authors.initials: JY
authors.lastname: Chu
authors.forename: A M
authors.initials: AM
authors.lastname: Felix
mesh_terms: D000596:Amino Acids; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D005449:Fluorenes; D010455:Peptides; D051381:Rats; D012116:Resins, Plant
chemical_list: D000596:Amino Acids; D005449:Fluorenes; C016456:N(alpha)-fluorenylmethyloxycarbonylamino acids; D010455:Peptides; D012116:Resins, Plant