Melanin-concentrating hormone: a structural and conformational study based on synthesis, biological activity, high-field NMR, and molecular modeling techniques.
A series of Melanin-concentrating hormone (MCH) fragments have been synthesized and their biological activities compared with the parent peptide. The substructural units, 5-14 linear and 5-14 cyclic, have been used as models for MCH-- H-Asp1-Thr-Met-Arg-Cys-Met-Val-Gly-Arg HO-Val17-Glu-Trp-Cys-Pro-Arg-Tyr-Val in 1H-nmr conformational studies. Conformational features predicted by molecular dynamics analyses find support in the nmr experiments.
Brown
D W
DW
University of Bath, Avon, England.
Campbell
M M
MM
Kinsman
R G
RG
White
P D
PD
Moss
C A
CA
Osguthorpe
D J
DJ
Paul
P K
PK
Baker
B I
BI
D000595:Amino Acid Sequence; D000818:Animals; D007028:Hypothalamic Hormones; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008543:Melanins / Q000138:chemical synthesis / Q000502:physiology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010907:Pituitary Hormones / Q000138:chemical synthesis / Q000502:physiology; D011487:Protein Conformation
D007028:Hypothalamic Hormones; D008543:Melanins; D010907:Pituitary Hormones; C019463:melanin-concentrating hormone