Characterization of plasma membrane polypeptides of Trypanosoma from bats.
Cell surface proteins of Trypanosoma dionisii, Trypanosoma vespertilionis and Trypanosoma sp. (M238) were radiodinated and their distribution both in the detergent-poor (DPP) and detergent-enriched phase (DRP) was studied using a phase separation technique in Triton X-114, as well as polyacrylamide gel electrophoresis in sodium dodecyl sulphate (SDS-PAGE). Significant differences were observed in the proteins present in the DRP when the three species of trypanosoma were compared. Two major bands with 88 and 70 KDa were observed in T. sp. (M238) but were not detectable in T. dionisii and T. vespertilionis. Three polypeptides with 96, 77 and 60 KDa were identified in the DRP of T. vespertilionis. Three major bands with 84, 72 and 60 KDa were observed in the DRP of T. dionisii. Two polypeptides with 34-36 KDa present in the DPP, were observed in the three Trypanosome species analyzed. Our observations show that T. sp. (M238) has characteristic surface polypeptides not found in T. dionisii and T. vespertilionis.
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Departamento de Imunologia, Instituto Oswaldo Cruz, Rio de Janeiro, Brasil.
de Simone
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D000818:Animals; D002685:Chiroptera / Q000469:parasitology; D003902:Detergents / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D008565:Membrane Proteins / Q000302:isolation & purification*; D017830:Octoxynol; D010455:Peptides / Q000302:isolation & purification*; D011092:Polyethylene Glycols / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology*; D014345:Trypanosoma / Q000032:analysis*
D003902:Detergents; D008565:Membrane Proteins; D010455:Peptides; D013501:Surface-Active Agents; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40