Comparative disposition of adriamycin delivered via magnetic albumin microspheres in presence and absence of magnetic field in rats.
The multiple tissue disposition of adriamycin hydrochloride delivered via magnetic albumin microspheres, in absence (control) and presence of magnetic field (experimental), has been investigated in rats. The animal tail was demarcated into three segments: T1, the dosing-site; T2, the target-site; and T3, the post target-site. Following the arterial cannulation at T1, 0.4 mg/kg of microsphere associated drug was administered to the control as well as the experimental animals. In experimental group, the target-site T2 was exposed to a 8000 G magnetic field for 30 min. In both groups the animals were sacrificed in triplicates over a 48 hr period and their various tissues monitored for drug concentrations using HPLC. In presence of magnetic field, the microspheres demonstrated 16 fold increase in the maximum drug concentration, 6 fold increase in drug exposure and 6 fold increase in the drug targeting efficiency for T2. Drug delivery to most non-target tissues, including heart and liver, was substantially reduced. The results quantitatively suggest that the efficacy of magnetic albumin microspheres in the targeted delivery of incorporated therapeutic agent is predominantly due to the magnetic effects, and not alone due to the characteristics of the micro-carrier system.
Gupta
P K
PK
Department of Pharmacy, University of Otago, Dunedin, New Zealand.
Hung
C T
CT
D000418:Albumins; D000818:Animals; D004317:Doxorubicin / Q000008:administration & dosage / Q000493:pharmacokinetics*; D004574:Electromagnetic Fields; D005260:Female; D008280:Magnetics; D008863:Microspheres; D009206:Myocardium / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013623:Tail / Q000378:metabolism; D014018:Tissue Distribution
D000418:Albumins; D004317:Doxorubicin