Increased glucose permeability in Babesia bovis-infected erythrocytes.
Glucose influx into bovine erythrocytes was found to be significantly increased upon infection with the parasite, Babesia bovis. The influx of glucose into the infected cells over 4 min was not saturable at high concentrations of glucose (240 mM), nor was it affected by established inhibitors of mammalian glucose transport, such as cytochalasin B and phloretin (0.1-100 microM). Glucose uptake into the parasitized cells was, however, inhibited by phloridzin (phloretin-2-beta-glucoside) at concentrations over the range of 10-500 microM. Further inhibition of glucose uptake by adenosine (2.5-15 mM) was found to occur in B. bovis-infected bovine erythrocytes, suggesting an interaction of adenosine with the new or altered component of glucose transport in the parasitized cells.
Upston
J M
JM
School of Biochemistry, University of New South Wales, Kensington, Australia.
Gero
A M
AM
D000818:Animals; D001403:Babesia / Q000502:physiology*; D002417:Cattle; D002478:Cells, Cultured; D004912:Erythrocytes / Q000378:metabolism / Q000469:parasitology*; D005947:Glucose / Q000378:metabolism*; D010539:Permeability
D005947:Glucose