Simplified procedure for purification of Bordetella bronchiseptica dermonecrotic toxin.
Bordetella bronchiseptica dermonecrotic toxin was purified by a simplified method. The method consisted of SP Toyopearl 650M chromatography and high performance liquid chromatography on a TSK gel G3000SW column. 47.5% of the activity of the crude cell extract was recovered. The purified toxin behaved as a homogeneous protein in sodium dodecyl sulfate polyacrylamide gel electrophoresis, high performance liquid chromatography, and agar gel double diffusion tests.
Horiguchi
Y
Y
Research Center for Veterinary Science, Kitasato Institute, Chiba, Japan.
Nakai
T
T
Kume
K
K
D000818:Animals; D001427:Bacterial Toxins / Q000302:isolation & purification* / Q000633:toxicity; D001884:Bordetella / Q000032:analysis*; D002845:Chromatography; D008970:Molecular Weight; D009336:Necrosis; D011108:Polymers; D012867:Skin / Q000187:drug effects / Q000473:pathology; D011503:Transglutaminases; D010566:Virulence Factors, Bordetella
D001427:Bacterial Toxins; D011108:Polymers; D010566:Virulence Factors, Bordetella; C060969:dermonecrotic toxin, Bordetella; C058606:Toyopearl 650; D011503:Transglutaminases