title: Characterization of aminopeptidases in human kidney soluble fraction.
abstract: Fractionation of human kidney soluble extract (with which the majority of cellular aminopeptidase activity is associated) via anion exchange chromatography resolved four types of separable aminopeptidase (relative activity in parenthesis): alanyl aminopeptidase (EC 3.4.11.14; 50%); arginyl aminopeptidase (EC 3.4.11.6; 30%); leucyl aminopeptidase (EC 3.4.11.1; 18%) and pyroglutamyl aminopeptidase (EC 3.4.19.3; 2%). The further purification (via gel filtration chromatography and preparative electrophoresis) and characterization of each aminopeptidase has been described; the aminopeptidase tissue profile for human kidney was found to be similar to that previously obtained for human skeletal muscle and brain tissue using a similar experimental approach, i.e. the same enzymes, with corresponding similar characteristics, are present in each tissue. These results suggest that soluble aminopeptidases may be of fundamental importance in general cell protein catabolism. Degradation of the following aminoacyl-AMC derivatives via kidney soluble extract has been shown to be due principally to hydrolysis by alanyl aminopeptidase: glutamyl, glycyl, isoleucyl, methionyl, ornithyl, phenylalanyl, prolyl, seryl, tyrosyl and valyl. We would suggest that measurement of the soluble extract-derived aminopeptidases described in this paper in urine may lead to an improvement upon existing assay procedures for early detection of kidney damage.
authors.affiliation: Regional Neurological Centre Molecular Genetics Unit, University of Newcastle upon Tyne, UK.
authors.forename: D
authors.initials: D
authors.lastname: Mantle
authors.forename: B
authors.initials: B
authors.lastname: Lauffart
authors.forename: J
authors.initials: J
authors.lastname: McDermott
authors.forename: A
authors.initials: A
authors.lastname: Gibson
mesh_terms: D000626:Aminopeptidases / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000201:enzymology*; D009842:Oligopeptides / Q000378:metabolism; D014020:Tissue Extracts / Q000032:analysis
chemical_list: D009842:Oligopeptides; D014020:Tissue Extracts; D000626:Aminopeptidases