Quantitation of blood plasma DNA as an index of in vivo cytotoxicity.
Cell death triggers the release into extracellular spaces of products of chromatin catabolism, particularly of DNA. A sensitive DNA assay has been used to investigate in the mouse whether the quantitation of plasma DNA may be used as an index of in vivo cytotoxicity. It has been found that toxic doses of bacterial lipopolysaccharide, HgCl2, CCl4, cyclophosphamide and hydroxyurea, are responsible for the release of extracellular DNA in plasma, in a dose dependent relationship. In conclusion, quantitation of extracellular DNA may be used for investigating in vivo cell death phenomena induced by toxic agents and drugs. Such a method could be applied to toxicological studies in animals and man.
Bret
L
L
Laboratoire d'Immunopathologie Rénale et d'Immunopharmacologie, Contrat Jeune Formation INSERM 88.01, Faculte de Medecine Purpan, Toulouse, France.
Lulé
J
J
Alary
C
C
Appolinaire-Pilipenko
S
S
Pourrat
J P
JP
Fournié
G J
GJ
D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects* / Q000502:physiology; D003520:Cyclophosphamide / Q000633:toxicity; D004247:DNA / Q000097:blood*; D004305:Dose-Response Relationship, Drug; D005110:Extracellular Space; D005260:Female; D006918:Hydroxyurea / Q000633:toxicity; D008070:Lipopolysaccharides / Q000633:toxicity; D008627:Mercuric Chloride / Q000633:toxicity; D008722:Methods; D051379:Mice
D008070:Lipopolysaccharides; D008627:Mercuric Chloride; D003520:Cyclophosphamide; D004247:DNA; D002251:Carbon Tetrachloride; D006918:Hydroxyurea