Adsorption of plasma proteins and adhesion of platelets onto novel polyetherurethaneureas--relationship between denaturation of adsorbed proteins and platelet adhesion.
Novel polyetherurethaneureas which have been synthesized by the present authors were chosen for the substrate polymers, on which adhesion of platelets was investigated. The number of adhered platelets and the amount of serotonin released from platelets adhered on the polymers and the protein-coated polymers were determined by radioisotope method. Both of them were enhanced with increasing content of urea linkages in the polyetherurethaneureas. The platelet adhesion was discussed in terms of the denaturation of plasma proteins upon adsorption, which was determined by Fourier-transform infrared spectroscopy. With increasing degree of protein denaturation, the platelet adhesion and the serotonin release were enhanced. This relationship was particularly evident in the case of albumin adsorption. It was shown that the surface properties of substrate polymers affect the protein adsorption, which in turn influences the adhesion of platelets.
Ito
Y
Y
Department of Polymer Chemistry, Faculty of Engineering, Kyoto University, Japan.
Sisido
M
M
Imanishi
Y
Y
D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials; D001792:Blood Platelets / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism*; D004285:Dogs; D010973:Platelet Adhesiveness; D011140:Polyurethanes; D011489:Protein Denaturation; D012701:Serotonin / Q000378:metabolism
D001672:Biocompatible Materials; D001798:Blood Proteins; D011140:Polyurethanes; D012701:Serotonin; C044690:polyetherurethane urea