Capillary electrophoresis with indirect amperometric detection.
The use of indirect amperometric detection with capillary electrophoresis is demonstrated. The system consists of a porous glass coupler which allows amperometric detection at a carbon fiber electrode placed in the end of the capillary. 3,4-Dihydroxybenzylamine is added to the buffer system as a continuously eluting electrophore. Indirect amperometric detection in 9-mumol I.D. capillaries provides detection limits as low as 380 attomole for the amino acid arginine. Finally, both direct and indirect amperometric detection can be accomplished simultaneously.
Olefirowicz
T M
TM
Department of Chemistry, Penn State University, University Park 16802.
Ewing
A G
AG
D000596:Amino Acids / Q000032:analysis; D004553:Electric Conductivity; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D010455:Peptides / Q000032:analysis
D000596:Amino Acids; D010455:Peptides