title: The effect of abomasal methionine supplementation on nitrogen retention of growing steers postruminally infused with casein or nonsulfur-containing amino acids.
abstract: An experiment was conducted to develop a system useful for measuring methionine requirements of growing steers. Seven ruminally cannulated steers (312 kg, gaining .91 kg/d) were fed a diet based on ammoniated corn cobs, corn starch, molasses and urea. Quantities of N and sulfur-containing amino acids disappearing from the small intestine were 96.0 and 14.8 g/d, respectively. Postruminal infusions of Na-caseinate (CAS) resulted in linear (P less than .01) increases in N retention with values increasing from 30.1 g/d with no postruminal CAS infusion to 39.3, 50.8 and 59.2 g/d (averaged across methionine supplementation) when 100, 200 and 300 g/d CAS were infused. Postruminal infusions of a mixture of crystalline L-amino acids (simulating the nonsulfur-containing essential amino acid pattern of casein; SIM) at levels of 100, 200 and 300 g/d also led to linear increases (P less than .01) in N retention with steers retaining 30.9, 38.9 and 50.5 g N/d (averaged across methionine supplementation), respectively. Postruminal infusion of 12 g/d L-methionine across CAS and SIM infusions improved (P less than .01) N retention by 7.6 g/d but infusion of SIM, which is devoid of sulfur amino acids, also increased N retention. Responses to methionine supplementation was greatest when 200 or 300 g/d SIM were abomasally infused. The data are interpreted to demonstrate that, for steers fed a diet containing little true protein, postruminal supplementation with nonsulfur-containing amino acids tended to increase the ability of growing steers to respond to methionine supplementation.
authors.affiliation: Dept. of Anim. Sci., University of Illinois, Urbana 61801.
authors.forename: E C
authors.initials: EC
authors.lastname: Titgemeyer
authors.forename: N R
authors.initials: NR
authors.lastname: Merchen
mesh_terms: D000018:Abomasum / Q000378:metabolism; D000596:Amino Acids / Q000008:administration & dosage / Q000378:metabolism*; D000818:Animals; D002364:Caseins / Q000008:administration & dosage / Q000378:metabolism*; D002417:Cattle / Q000378:metabolism*; D007421:Intestine, Small / Q000378:metabolism; D008297:Male; D008715:Methionine / Q000008:administration & dosage / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism*; D012417:Rumen / Q000378:metabolism
chemical_list: D000596:Amino Acids; D002364:Caseins; D008715:Methionine; D009584:Nitrogen