Nonrandom induction of pyrimidine-pyrimidone (6-4) photoproducts in ultraviolet-irradiated human chromatin.
Radioimmunoassays that detect pyrimidine-pyrimidone (6-4) photoproducts and cyclobutane dimers were used to determine the relative induction of these photoproducts in nucleosomal (core) and internucleosomal (linker) DNA in human cell chromatin irradiated with UV light. Cyclobutane dimers were formed in equal amounts/nucleotide in core and linker DNA, whereas (6-4) photoproducts occurred with 6-fold greater frequency/nucleotide in linker DNA.
Mitchell
D L
DL
Laboratory of Radiobiology and Environmental Health, University of California, San Francisco 94143-0750.
Nguyen
T D
TD
Cleaver
J E
JE
D002460:Cell Line; D002843:Chromatin / Q000378:metabolism / Q000528:radiation effects*; D004247:DNA / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D009707:Nucleosomes / Q000378:metabolism; D010777:Photochemistry; D011740:Pyrimidine Dimers / Q000378:metabolism*; D011863:Radioimmunoassay; D014466:Ultraviolet Rays
D002843:Chromatin; D009707:Nucleosomes; D011740:Pyrimidine Dimers; C058406:pyrimidine-pyrimidone dimer; D004247:DNA