title: Gap junctions in the hypothalamic arcuate neurons of ovariectomized and estradiol-treated rats.
abstract: Freeze-fracture methodology was used to study the organization of the neuronal plasma membrane in the rat arcuate nucleus, an estrogen sensitive area of the hypothalamus. Freeze-fracture replicas were prepared from 6 adult ovariectomized rats injected with a single dose of 17 beta-estradiol and from 6 ovariectomized littermates injected with vehicle. Rats were sacrificed 2 days after the injection. Occasional gap junctions were observed in freeze-fractured neuronal membranes from both groups of animals and their incidence was increased (P less than 0.01) in estradiol treated rats. This study demonstrates gap junctions in arcuate neurons and suggests that these structures may be affected by gonadal hormones.
authors.affiliation: Instituto Cajal, C.S.I.C., Madrid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Perez
authors.forename: P A
authors.initials: PA
authors.lastname: Tranque
authors.forename: F
authors.initials: F
authors.lastname: Naftolin
authors.forename: L M
authors.initials: LM
authors.lastname: Garcia-Segura
mesh_terms: D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000187:drug effects / Q000648:ultrastructure*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005614:Freeze Fracturing; D007365:Intercellular Junctions / Q000187:drug effects / Q000648:ultrastructure*; D010052:Ovariectomy; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004958:Estradiol