The effect of inhibitors of prostaglandin formation on contraction of the rat, rabbit and human vas deferens.
1. The rat vas deferens releases both PGE2 and PGF2 alpha under basal conditions in vitro but the human vas deferens synthesizes prostaglandins only when arachidonic acid is supplied exogenously. 2. The release of PGE2 and PGF2 alpha is augmented by alpha-adrenoceptor activation or by BaCl2, both of which cause contraction. 3. Release of PGE2 and PGF2 alpha is substantially inhibited by indomethacin 10 micrograms ml-1 which does not affect contraction. 4. Contraction is strongly inhibited by higher concentrations of indomethacin (50-80 micrograms ml-1). 5. It is concluded that the release of PGE2 and PGF2 alpha from the rat and human vas deferens is not correlated with contraction.
Patra
P B
PB
Department of Physiology and Pharmacology, University of Strathclyde, Glasgow, Scotland.
Wadsworth
R M
RM
Hay
D W
DW
Zeitlin
I J
IJ
D000818:Animals; D015237:Dinoprost / Q000096:biosynthesis*; D015232:Dinoprostone / Q000096:biosynthesis*; D000431:Ethanol / Q000494:pharmacology; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D014649:Vas Deferens / Q000187:drug effects* / Q000378:metabolism
D000431:Ethanol; D015237:Dinoprost; D015232:Dinoprostone; D007213:Indomethacin