Production of monoclonal antibody against a hemolysin (Vp-TRH) produced by Vibrio parahaemolyticus.
The antigenicity of a hemolysin (Vp-TRH: Vp-TDH related hemolysin) produced by Kanagawa phenomenon-negative clinical isolates of Vibrio parahaemolyticus was studied using monoclonal antibodies (MAbs). A total of 12 hybridoma clones which produced MAbs against Vp-TRH were established. All MAbs contained the Kappa light chain and were IgG type. These MAbs were divided into a minimum of 5 different specificity groups, including antibodies specific to Vp-TRH and common to both Vp-TRH and Vp-TDH, a possible pathogenic toxin of Kanagawa phenomenon-positive V. parahaemolyticus. These results clearly show the immunological similarity and dissimilarity (specificity) of Vp-TRH and Vp-TDH.
Honda
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Research Institute for Microbial Diseases, Osaka University, Japan.
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Miwatani
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D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000942:Antigens, Bacterial; D015153:Blotting, Western; D003429:Cross Reactions; D006460:Hemolysin Proteins / Q000276:immunology*; D014736:Vibrio parahaemolyticus / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D006460:Hemolysin Proteins