Analysis of bumetanide in human urine by high-performance liquid chromatography with fluorescence detection and gas chromatography/mass spectrometry.
Bumetanide is a potent diuretic. Its concentration in urine after therapeutic doses is in the low ng/mL range. A procedure using reversed-phase HPLC with fluorescence detection is described. Using this procedure, the authors have been able to measure bumetanide in a urine sample at 10 ng/mL with a signal-to-noise ratio of 7. Therefore, the detection limit is lower than this concentration. Confirmation is by a GC/MS procedure after methylating the diuretic. Derivatization is by extractive alkylation with tetrabutylammonium hydrogen sulfate as the phase transfer reagent and iodomethane as the methylating agent.
Gradeen
C Y
CY
Department of Laboratory Medicine, Foothills Hospital, Calgary, Alberta, Canada.
Billay
D M
DM
Chan
S C
SC
D002034:Bumetanide / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D004232:Diuretics / Q000652:urine*; D004300:Doping in Sports; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D013050:Spectrometry, Fluorescence; D015813:Substance Abuse Detection / Q000379:methods*
D004232:Diuretics; D002034:Bumetanide