The "lecithotrophic" sea urchin Heliocidaris erythrogramma lacks typical yolk platelets and yolk glycoproteins.
The sea urchin Heliocidaris tuberculata undergoes typical development, forming an echinoid pluteus larva, whereas H. erythrogramma undergoes direct development via a highly modified, nonfeeding larva. Using a polyclonal antibody prepared against yolk glycoproteins from the typical developer Stronglyocentrotus purpuratus, we found that H. tuberculata contains cross-reactive proteins in abundance, but H. erythrogramma does not. In addition, we used immunoelectron microscopy to demonstrate that unfertilized eggs of H. tuberculata contain yolk platelets, but those of H. erythrogramma do not.
Scott
L B
LB
Department of Biochemistry and Molecular Biology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Lennarz
W J
WJ
Raff
R A
RA
Wray
G A
GA
D000818:Animals; D005075:Biological Evolution; D015153:Blotting, Western; D004527:Egg Proteins / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis*; D008854:Microscopy, Electron; D008970:Molecular Weight; D015388:Organelles / Q000648:ultrastructure; D010063:Ovum / Q000032:analysis / Q000648:ultrastructure*; D012617:Sea Urchins / Q000502:physiology* / Q000648:ultrastructure
D004527:Egg Proteins; D006023:Glycoproteins