Stimulation of protein secretion in the initial segment of the rat epididymis by fluid from the ram rete testis.
Zone 1A of the ductus epididymidis was perfused with ovine rete testis fluid (nRTF) and modifications of it, and a synthetic medium (sRTF) based on the inorganic composition of nRTF. There was little fluid transport by the duct mucosa and nRTF stimulated protein secretion. The secretagogue activity was not extracted by charcoal, was sensitive to protease digestion and was present in a portion of nRTF with a molecular weight of greater than 10,000. The addition of bovine serum albumin to the sRTF stimulated protein secretion, but not to the same extent as equal amounts of protein in nRTF. Polyacrylamide gel electrophoresis of the perfusates showed that proteins with molecular weights of 19,000 (all rats studied), and 22,000, 30,000 and 60,000 (at least half the rats studied) were secreted into the perfusion fluids as well as some blood proteins, but the pattern of secretion was not affected by the composition of the perfusion fluid.
Sujarit
S
S
Department of Biological Sciences, University of Newcastle, New South Wales, Australia.
Jones
R C
RC
Setchell
B P
BP
Chaturapanich
G
G
Lin
M
M
Clulow
J
J
D000818:Animals; D001826:Body Fluids / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004822:Epididymis / Q000033:anatomy & histology / Q000378:metabolism*; D008297:Male; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012756:Sheep; D013737:Testis / Q000378:metabolism*
D011506:Proteins