title: A new antileishmanial compound, phaseolinone.
abstract: Inclusion of phaseolinone, a newly described mycotoxin, at 20 micrograms per ml in a solid culture medium (blood agar overlay) and at 50 micrograms per ml in a liquid culture (medium 199) inhibited the growth of L. donovani promastigotes. About 90% of the motile promastigotes lost motility after exposure to 50 micrograms per ml of phaseolinone for 6-7 h and here 3-day-old culture was more sensitive than 7-day-old culture. In an in vitro assay, DNA dependent RNA polymerase activity of 3-day-old promastigotes was considerably inhibited in the presence of this toxin. Therefore, this key enzyme was suggested to be one of the sites of action of phaseolinone.
authors.affiliation: Department of Cellular Biochemistry, Indian Institute of Chemical Biology, Calcutta.
authors.forename: R
authors.initials: R
authors.lastname: Roy
authors.forename: G
authors.initials: G
authors.lastname: Bhattacharya
authors.forename: K A
authors.initials: KA
authors.lastname: Siddiqui
authors.forename: R
authors.initials: R
authors.lastname: Bhadra
mesh_terms: D000818:Animals; D000981:Antiprotozoal Agents; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors; D007893:Leishmania donovani / Q000187:drug effects*; D009183:Mycotoxins / Q000494:pharmacology*; D009284:Naphthols / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D000981:Antiprotozoal Agents; D009183:Mycotoxins; D009284:Naphthols; C056401:phaseolinone; D012321:DNA-Directed RNA Polymerases