Persistent spreading of ligament cells on osteopontin/bone sialoprotein-I or collagen enhances tolerance to heat shock.
Fibronectin (FN), bone sialoprotein-I (BSP-I), Type I collagen, and a number of synthetic peptides containing the integrin attachment sequence (RDG) were evaluated for their ability to affect stress tolerance in osteo-ligament cells (OL). The attachment and spreading of OL cells was determined by the method of Klebe (1974) and Akiyama et al. (1986). Survival from heat shock was evaluated after the methods of Gerner et al. (1976). These studies showed that FN, BSP-I, and synthetic RGD peptides enhance attachment of OL cells. Increased survival from heat was limited to cells spread on fibronectin, BSP-I, and Type I collagen. OL cells that persistently spread on BSP-I and Type I collagen had more survivors than cells demonstrating transient spreading on FN. These studies indicate that (a) cell spreading is a prerequisite for stress tolerance and (b) enhanced stress tolerance is mediated by protein sequences other than those immediately surrounding the RGD sites in native proteins.
Sauk
J J
JJ
Department of Pathology, Dental School, University of Maryland, Baltimore 21201.
Van Kampen
C L
CL
Norris
K
K
Moehring
J
J
Foster
R A
RA
Somerman
M J
MJ
D000818:Animals; D002465:Cell Movement / Q000502:physiology; D002478:Cells, Cultured; D003094:Collagen / Q000502:physiology*; D004285:Dogs; D005109:Extracellular Matrix / Q000502:physiology; D006360:Heat-Shock Proteins / Q000502:physiology*; D008022:Ligaments / Q000166:cytology* / Q000502:physiology; D053495:Osteopontin; D012795:Sialoglycoproteins / Q000502:physiology*; D013696:Temperature
D006360:Heat-Shock Proteins; D012795:Sialoglycoproteins; D053495:Osteopontin; D003094:Collagen