Transfer of the toxin protein genes of Bacillus sphaericus into Bacillus thuringiensis subsp. israelensis and their expression.
The genes encoding the toxic determinants of Bacillus sphaericus have been expressed in a nontoxic and a toxic strain of Bacillus thuringiensis subsp. israelensis. In both cases, the B. sphaericus toxin proteins were produced at a high level during sporulation of B. thuringiensis and accumulated as crystalline structures. B. thuringiensis transformants expressing B. sphaericus and B. thuringiensis subsp. israelensis toxins did not show a significant enhancement of toxicity against Aedes aegypti, Anopheles stephensi, and Culex pipiens larvae.
Bourgouin
C
C
Unité de Biochimie Microbienne, Institut Pasteur, Paris, France.
Delécluse
A
A
de la Torre
F
F
Szulmajster
J
J
D000330:Aedes; D000818:Animals; D000852:Anopheles; D001407:Bacillus / Q000235:genetics* / Q000502:physiology / Q000648:ultrastructure; D001413:Bacillus thuringiensis / Q000235:genetics* / Q000502:physiology / Q000648:ultrastructure; D001427:Bacterial Toxins / Q000235:genetics*; D003001:Cloning, Molecular; D003465:Culex; D015964:Gene Expression Regulation, Bacterial; D005822:Genetic Vectors; D007814:Larva; D008854:Microscopy, Electron; D010957:Plasmids; D015183:Restriction Mapping; D013171:Spores, Bacterial; D014169:Transformation, Bacterial
D001427:Bacterial Toxins; C046134:binB protein, Bacillus sphaericus