A transcription stimulatory factor binds to the upstream region of Xenopus 5 S RNA and tRNA genes.
Upstream sequence elements modulate transcription efficiency and competition strength of eucaryotic 5 S rRNA and tRNA genes transcribed by RNA polymerase III. We report on the chromatographic separation and functional characterization of at least two components involved in this process: one has a DNA binding activity and interacts with the immediate upstream region of Xenopus tRNA and 5 S rRNA genes, and a second component, which by itself does not interact with the upstream region of these genes, is required together with the DNA binding activity for transcription stimulation in vitro.
Oei
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SL
Max-Planck-Institut für Molekulare Genetik, Otto-Warburg-Laboratorium, Berlin, Federal Republic of Germany.
Pieler
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D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D005260:Female; D005796:Genes; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010053:Ovary / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012335:RNA, Ribosomal / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D012343:RNA, Transfer / Q000235:genetics*; D014157:Transcription Factors / Q000302:isolation & purification / Q000378:metabolism*; D014158:Transcription, Genetic; D014982:Xenopus laevis
D012335:RNA, Ribosomal; D012341:RNA, Ribosomal, 5S; D014157:Transcription Factors; D012343:RNA, Transfer