Turnover of [14C] sucrose HDL and uptake by organs in the normal or genetically hypercholesterolemic (RICO) rat using a constant infusion method.
The turnover and tissular uptake of HDL (d 1.095-1.21) have been compared in normocholesterolemic or genetically hypercholesterolemic rats by a constant infusion method of [14C] sucrose labelled HDL for 8 h. The HDL clearance rate was not significantly smaller in the RICO than in the normocholesterolemic animal (320 +/- 22 microliters.h-1 versus 366 +/- 24 microliters.h-1 per 100 g of rat). It was the same case for the fractional catabolic rate, respectively equal to 7.8 and 9.4 +/- 0.6%.h-1. For both strains, liver and skeletal muscle were the main catabolic sites for HDL. The HDL uptake rates in intestine or kidney were 3-4-fold smaller than those in the liver. In the RICO rat, intestine, testis and adrenals showed a lesser HDL uptake capacity (expressed per g of organ) than the normocholesterolemic rat.
Lutton
C
C
Laboratoire de Physiologie de la Nutrition, URA D.0646 CNRS, Université de Paris-Sud, Orsay, France.
Ouguerram
K
K
Sauvage
M
M
Magot
T
T
D000818:Animals; D002250:Carbon Radioisotopes; D002784:Cholesterol / Q000097:blood; D006937:Hypercholesterolemia / Q000378:metabolism*; D007262:Infusions, Intravenous; D008075:Lipoproteins, HDL / Q000008:administration & dosage / Q000493:pharmacokinetics*; D008297:Male; D051381:Rats; D011922:Rats, Mutant Strains / Q000378:metabolism*; D013395:Sucrose; D014018:Tissue Distribution
D002250:Carbon Radioisotopes; D008075:Lipoproteins, HDL; D013395:Sucrose; D002784:Cholesterol