Susceptibility of RDM4 lymphoma cells to LAK-mediated lysis is decreased in tumor bearers fed fish oil high fat regimen.
RDM4 lymphoma cells were grown intraperitoneally in genetically compatible AKR mice fed either regular mouse chow, or diet supplemented with either saturated fat (hydrogenated beef tallow = HBT) or unsaturated fat (fish oil = FO). It was observed that the lymphoma cells number was significantly greater in FO-fed hosts and lower in HBT-fed hosts, than in the mice fed regular chow. The tumor bearers diet did not dramatically influence the rate of DNA synthesis of RDM4 cells, as measured by [3H]thymidine uptake in culture, a few hours after harvesting from the peritoneal cavity. It was repeatedly found that FO feeding of the tumor bearers elicited an increased resistance of RDM4 cells to lysis by LAK effectors, as appraised in vitro by 51Cr release test and in vivo by the "Winn assay". Different FO percentage of the diet (16%, 8%, 4%) resulted in comparable reduction of susceptibility of RDM4 cells to lysis by LAK effectors. Lipid analysis showed that RDM4 cells grown in mice fed FO diet or HBT diet differed markedly in their fatty acid composition and that their resistance to lysis by LAK cells correlated with the quantity of oxidizable fatty acids especially of the n-6 type.
el Ayachi
N
N
Centre de recherche en immunologie, Institut Armand-Frappier, Laval-des-Rapides, Quebec, Canada.
Begin
M
M
Mercier
D
D
Ells
G
G
Oth
D
D
D000818:Animals; D002455:Cell Division; D003602:Cytotoxicity, Immunologic; D004041:Dietary Fats / Q000008:administration & dosage*; D005227:Fatty Acids / Q000032:analysis; D005395:Fish Oils / Q000494:pharmacology*; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology*; D015227:Lipid Peroxidation; D008223:Lymphoma / Q000378:metabolism / Q000473:pathology*; D008563:Membrane Lipids / Q000032:analysis; D051379:Mice; D008806:Mice, Inbred AKR; D014407:Tumor Cells, Cultured
D004041:Dietary Fats; D005227:Fatty Acids; D005395:Fish Oils; D008563:Membrane Lipids