title: Comparative study of immunocytochemical staining versus Giemsa stain for detecting dysmegakaryopoiesis in myelodysplastic syndromes (MDS)
abstract: 23 patients with myelodysplastic syndromes (MDS) and 8 normal controls were analyzed for dysmegakaryopoiesis (DMP) in the bone marrow by alkaline phosphatase anti-alkaline phosphatase (APAAP) technique and by conventional May-Giemsa staining. In the immunocytochemical study, monoclonal antibody (MoAb) against glycoprotein (GP) IIb/IIIa was utilized to demonstrate megakaryocytic cells. 91% (21/23) of MDS cases were detected as having DMP by APAAP method, while only 52% (12/23) were detectable by Giemsa stain. There were difficulties in recognizing small micromegakaryocytes (micro MKs), designated as type 1 atypical MKs, by Giemsa staining. Furthermore, megakaryoblasts (MKBs) were detectable only by APAAP technique. In 8 normal controls, no type 1 and type 3 atypical MKs (round shaped multinuclear MKs) were observed either by Giemsa staining or by the APAAP method, suggesting that they are a distinctive feature of MDS. These results indicate the necessity of immunocytochemical technique for accurate recognition of DMP in MDS.
authors.affiliation: First department of Internal Medicine, Tokyo Medical College, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kawaguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Nehashi
authors.forename: S
authors.initials: S
authors.lastname: Aizawa
authors.forename: K
authors.initials: K
authors.lastname: Toyama
mesh_terms: D000740:Anemia / Q000175:diagnosis / Q000473:pathology; D001399:Azure Stains; D001853:Bone Marrow / Q000473:pathology*; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008533:Megakaryocytes / Q000473:pathology*; D009190:Myelodysplastic Syndromes / Q000175:diagnosis / Q000473:pathology*; D012016:Reference Values
chemical_list: D001399:Azure Stains