Two ELISA methods for the measurement of airborne swine epithelial antigens.
A new double antibody sandwich ELISA for measuring swine epithelial antigens in swineries was compared to an adaption of our previously developed ELISA inhibition method. The sandwich ELISA is more sensitive than the inhibition ELISA, and its reproducibility is better. The quantitative results from swinery samples of the sandwich ELISA are about ten times higher, but correlate well (rs 0.88, p = 0.004) with the results of ELISA inhibition. The progression in magnitude of the results in relation to the sample dilution was examined.
Virtanen
T I
TI
Department of Clinical Microbiology, University of Kuopio, Finland.
D000395:Air Pollutants, Occupational / Q000032:analysis*; D000485:Allergens / Q000032:analysis*; D000818:Animals; D000906:Antibodies; D000941:Antigens / Q000032:analysis; D001710:Biotin; D004797:Enzyme-Linked Immunosorbent Assay; D004848:Epithelium / Q000276:immunology; D006799:Housing, Animal; D013552:Swine / Q000276:immunology*
D000395:Air Pollutants, Occupational; D000485:Allergens; D000906:Antibodies; D000941:Antigens; D001710:Biotin