[Clinical laboratory application of HPLC].
In clinical laboratory tests, high performance liquid chromatography (HPLC) has been widely used because of good reproducibility of data and easy automatization. In this workshop, separation of IgG and albumin in commercially available IgG preparation and determination of blood theophylline and serum uric acid levels were conducted by the HPLC method. In separation of IgG and albumin, HPLC analysis was carried out within 10 minutes by using the high-performance gel filtration column (TSK gel G 3,000 SWXL). Blood theophylline was fully automatically determined by using the pretreated column to separate small molecular material before application of the analytical column. A good correlation of blood theophylline levels between HPLC and ordinary EIA method was observed. In determination of serum uric acid, pretreatment for deproteinization of samples by acid was done to measure exactly the whole blood uric acid content. Eluent was adjusted to pH 2.2 to increase affinity to ODS phase of the reversed column (TSK gel ODS-80 TM). There was a good correlation of values of uric acid between HPLC and uricase-catalase method.
Kawanishi
K
K
Department of Clinical Laboratory, Kagawa Medical School.
D000418:Albumins / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D013806:Theophylline / Q000097:blood; D014527:Uric Acid / Q000097:blood
D000418:Albumins; D007074:Immunoglobulin G; D014527:Uric Acid; D013806:Theophylline