Natural killer cell-mediated lysis of herpes simplex virus-infected fibroblasts: inability to detect soluble factors that contribute to lysis.
We investigated the role of soluble factors in natural killer (NK) cell-mediated lysis of herpes simplex virus (HSV)-infected cells. Supernatants generated by incubating human peripheral blood mononuclear cells with HSV-infected human fibroblasts contained tumor necrosis factor (TNF) and lysed uninfected U937 cells, but not HSV-infected fibroblasts. U937 cells, but not HSV-infected fibroblasts, were lysed when exposed to recombinant TNF (rTNF) for 18 hr. NK cell-mediated lysis of HSV-infected fibroblasts was not inhibited by addition of anti-TNF or anti-lymphotoxin (LT) antibodies to cytotoxicity assays. Thus, a role for soluble factors, and in particular TNF and LT, in NK cell-mediated lysis of HSV-infected cells could not be demonstrated.
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Division of Infectious Diseases, Joseph Stokes, Jr. Research Institute, Children's Hospital of Philadelphia, Pennsylvania 19104.
Trinchieri
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Bandyopadhyay
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Starr
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D003602:Cytotoxicity, Immunologic; D003603:Cytotoxins / Q000032:analysis*; D005347:Fibroblasts; D006561:Herpes Simplex / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D007694:Killer Cells, Natural / Q000276:immunology*; D008233:Lymphotoxin-alpha / Q000032:analysis / Q000502:physiology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000502:physiology
D003603:Cytotoxins; D008233:Lymphotoxin-alpha; D014409:Tumor Necrosis Factor-alpha