Paralog chromatography.
As a mixed mode ligand, a small peptide can mimic an antibody's paratope (antigen recognition site). This report describes the construction of a representative set of paratope analogs, or "paralogs," which can be conjugated to a chromatographic sorbent to combine desirable characteristics of traditional high-performance liquid chromatography columns with the specificity of a moderate affinity antibody. The broad utility of this novel set of protein separatory reagents is illustrated on the complex mixture of proteins in a yeast lysate.
Kauvar
L M
LM
Terrapin Technologies, Inc., San Francisco, CA 94103.
Cheung
P Y
PY
Gomer
R H
RH
Fleischer
A A
AA
D000906:Antibodies; D002458:Cell Fractionation; D002845:Chromatography / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000276:immunology / Q000302:isolation & purification*; D010455:Peptides / Q000276:immunology; D011487:Protein Conformation; D015003:Yeasts
D000906:Antibodies; D005656:Fungal Proteins; D010455:Peptides