Fibroblast mitogens in swine milk include an epidermal growth factor-related peptide.
Mammary secretions obtained from lactating sows can support in vitro growth of mammalian cells when added to Dulbecco's Modified Eagle Medium. In order to identify the growth factors present within, sow milk was fractionated and fractions having mitogenic activity were identified by their ability to stimulate DNA synthesis in density-arrested, quiescent (murine) AKR-2B fibroblasts. A prominent mitogenic activity (peak III) was observed in the 3,000-5,000 Mr range. This activity was partially purified by (1) preparative Sephadex G-200 chromatography, (2) gel-filtration in Sephadex G-50 columns and (3) DEAE-cellulose anion exchange chromatography. The last step resolved peak III activity into at least two distinct components. One component was highly-purified by use of reverse-phase high-performance liquid chromatography (RP-HPLC). This activity was identified as an epidermal growth factor (EGF)-related peptide based on its inactivation by polyclonal antibody (IgG fraction) specific for murine EGF (mEGF) and proteolytic agents. The other component is unrelated to EGF. Using cloned mEGF cDNA as a probe, the presence of EGF-related mRNA in lactating mammary tissues and newborn pig small intestine was also demonstrated. These factors may contribute to the preferential growth of gastrointestinal tissues in neonatal pigs.
Tan
T J
TJ
Department of Animal Science, Ohio Agricultural Research and Development Center, Ohio State University, Wooster.
Schober
D A
DA
Simmen
F A
FA
D000818:Animals; D015152:Blotting, Northern; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D015342:DNA Probes; D004815:Epidermal Growth Factor / Q000032:analysis* / Q000235:genetics; D005260:Female; D005347:Fibroblasts; D007774:Lactation; D008892:Milk / Q000032:analysis*; D009928:Organ Specificity; D010455:Peptides / Q000032:analysis* / Q000235:genetics; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D013552:Swine
D015342:DNA Probes; D010455:Peptides; D012333:RNA, Messenger; D004815:Epidermal Growth Factor