title: The effect of chylomicron remnants on bile acid synthesis in cultured rat hepatocytes.
abstract: The effect of chylomicron remnants on bile acid synthesis in isolated rat hepatocytes in monolayer cultures was investigated. Production of bile acids by the cells in the presence of chylomicron remnants at a cholesterol concentration of 7.8-9 nmol/ml was increased by approx. 75% after 17 h and 25% after 24 h incubation. Similar concentrations of cholesterol added to the cells in the form of chylomicrons had no significant effect on bile acid synthesis. These results suggest that cholesterol taken up in chylomicron remnants may be an important source of substrate for bile acid synthesis.
authors.affiliation: Department of Veterinary Basic Sciences, Royal Veterinary College, London, U.K.
authors.forename: R P
authors.initials: RP
authors.lastname: Fry
authors.forename: P A
authors.initials: PA
authors.lastname: Mayes
authors.forename: K E
authors.initials: KE
authors.lastname: Suckling
authors.forename: K M
authors.initials: KM
authors.lastname: Botham
mesh_terms: D000818:Animals; D001647:Bile Acids and Salts / Q000096:biosynthesis*; D002478:Cells, Cultured; D002784:Cholesterol / Q000494:pharmacology; D002914:Chylomicrons / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014361:Trypsin Inhibitors
chemical_list: D001647:Bile Acids and Salts; D002914:Chylomicrons; D014361:Trypsin Inhibitors; D002784:Cholesterol