Sequence comparison of the phosphoprotein mRNAs of antigenic subgroups A and B of human respiratory syncytial virus identifies a highly divergent domain in the predicted protein.
The sequences of the P mRNA and protein of strain 18537 of antigenic subgroup B of human respiratory syncytial virus were determined by sequencing cloned cDNAs of intracellular mRNA. Comparison with the corresponding sequences of the A2 strain of subgroup A showed that there was extensive sequence identity at both the nucleotide (80% identity) and amino acid (90% identity) levels. The P proteins contained a single divergent region (52% amino acid identity) flanked by highly conserved domains (96% identity). The previously observed differences in electrophoretic mobilities between the P proteins of subgroup A strains and certain subgroup B strains could not be attributed to differences in the polypeptide Mr of the primary translation product.
Johnson
P R
PR
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Collins
P L
PL
D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000235:genetics; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D015707:HN Protein; D006801:Humans; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D012136:Respiratory Syncytial Viruses / Q000235:genetics* / Q000276:immunology; D014758:Viral Core Proteins / Q000235:genetics*; D014759:Viral Envelope Proteins; D014764:Viral Proteins
D000956:Antigens, Viral; D004279:DNA, Viral; D015707:HN Protein; D010750:Phosphoproteins; D012333:RNA, Messenger; D012367:RNA, Viral; D014758:Viral Core Proteins; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C116204:attachment protein G