Effect of clofibrate on peroxisomal lignoceroyl-CoA ligase activity.
The effect of a 2-week clofibrate (0.5%)-fortified diet on peroxisomal palmitoyl-CoA and lignoceroyl-CoA ligases was studied. The activities of palmitoyl-CoA and lignoceroyl-CoA ligases in peroxisomes isolated from clofibrate-treated animals were 4.4- and 4.0-fold higher than those of the controls. The different degrees of increases in these two enzyme activities support the previous conclusions that in peroxisomes palmitoyl-CoA ligase and lignoceroyl-CoA ligase are different enzymes. Since clofibrate treatment increases both of these peroxisomal acyl-CoA ligase activities and normal palmitoyl-CoA ligase is the source of the partial activity for the oxidation of lignoceric acid in X-ALD, treatment with a hypolipidemic drug, which can increase human peroxisomal enzyme activities, may be helpful in lowering the amount of the pathogen, VLC fatty acids, in X-ALD.
Yoshida
Y
Y
Department of Pediatrics, Medical University of South Carolina, Charleston 29425.
Singh
I
I
D000818:Animals; D015415:Biomarkers; D002467:Cell Nucleus / Q000378:metabolism; D002994:Clofibrate / Q000494:pharmacology*; D003066:Coenzyme A Ligases / Q000378:metabolism*; D004032:Diet; D005227:Fatty Acids / Q000378:metabolism; D008297:Male; D008830:Microbodies / Q000187:drug effects / Q000201:enzymology*; D008862:Microsomes, Liver / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology; D009245:NADH Dehydrogenase / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000187:drug effects / Q000201:enzymology
D015415:Biomarkers; D005227:Fatty Acids; D009245:NADH Dehydrogenase; D003066:Coenzyme A Ligases; C044125:lignoceroyl-CoA ligase; D002994:Clofibrate