title: Dendritic cells and class II MHC expression on thyrocytes during the autoimmune thyroid disease of the BB rat.
abstract: The BB rat is a well-established model for spontaneous thyroid autoimmune disease. Since antigen presentation in thyroid autoimmunity is still a matter of debate, we studied the presence of antigen-presenting dendritic cells in the thyroid of the BB/O rat during the development of the disease in relation to the presence of other leucocytes and the aberrant expression of class II MHC determinants by thyrocytes. Thyroid glands, as well as thyroid-draining lymph nodes, were investigated in enzyme histochemistry and immune histochemistry. The appearance of anti-colloid antibodies in the circulation at 6 weeks of age was accompanied by an increase in the weight of the thyroid-draining cervical lymph nodes, which contained many anti-thyroglobulin-producing plasma cells. The only noteworthy event in the thyroid gland in this early stage of the disease was an increase in the number of dendritic cells. T cells, B cells, and plasma cells were virtually absent from the thyroid, and thyrocytes were invariably negative for class II MHC determinants. Only after 18 weeks of age, when large accumulations of dendritic cells, B lymphocytes, and T lymphocytes were seen in 40% of the BB thyroids, could some class II MHC positive thyroicytes be observed. At this stage the thyroid also contained some anti-thyoglobulin-producing plasma cells. Our observations suggest that dendritic cells play a role in antigen presentation in the early stages of the thyroid autoimmune response.
authors.affiliation: Department of Pathology, Free University Hospital, Amsterdam, The Netherlands.
authors.forename: H A
authors.initials: HA
authors.lastname: Voorby
authors.forename: P J
authors.initials: PJ
authors.lastname: Kabel
authors.forename: M
authors.initials: M
authors.lastname: de Haan
authors.forename: P H
authors.initials: PH
authors.lastname: Jeucken
authors.forename: R D
authors.initials: RD
authors.lastname: van der Gaag
authors.forename: M H
authors.initials: MH
authors.lastname: de Baets
authors.forename: H A
authors.initials: HA
authors.lastname: Drexhage
mesh_terms: D000818:Animals; D015551:Autoimmunity; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D008198:Lymph Nodes / Q000503:physiopathology; D008221:Lymphoid Tissue / Q000254:growth & development*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013961:Thyroid Gland / Q000276:immunology* / Q000473:pathology; D013967:Thyroiditis, Autoimmune / Q000276:immunology*
chemical_list: D000949:Histocompatibility Antigens Class II