Biotransformation of substituted benzoates to the corresponding cis-diols by an engineered strain of Pseudomonas oleovorans producing the TOL plasmid-specified enzyme toluate-1,2-dioxygenase.
The conversion of substituted benzoates into 1,2-cis-dihydroxycyclohexa-3,5-diene carboxylic acids (cis-diols) was effected by using Escherichia coli and Pseudomonas recombinants carrying the xylXYZ genes originating from the Pseudomonas putida mt-2 TOL plasmid, thus producing toluate-1,2-dioxygenase. Pseudomonas oleovorans GPo12 recombinants readily produced meta- and para-substituted cis-diols, but were limited in their oxidation of ortho-substituted substrates.
Wubbolts
M G
MG
Department of Medical Biochemistry, Faculty of Medicine, University of Geneva, Switzerland.
Timmis
K N
KN
D001565:Benzoates / Q000378:metabolism*; D001711:Biotransformation; D015964:Gene Expression Regulation, Bacterial; D005818:Genetic Engineering; D010105:Oxygenases / Q000096:biosynthesis* / Q000235:genetics; D010957:Plasmids; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
D001565:Benzoates; D010105:Oxygenases; C023172:toluene dioxygenase