Major thiobarbituric acid-reactive substances of liver homogenate are alkadienals.
Thiobarbituric acid (TBA) assay of rat liver homogenate was performed by four general variations with 0.01% butylated hydroxytoluene. Development of red pigment was greatly dependent on the methods. The pigment formation by each method was dramatically increased by introduction of 2 mM t-butyl hydroperoxide (t-BuOOH). The pH values of the reaction mixtures greatly affected the pigment yield both in the absence and presence of t-BuOOH, and the maximal pigment yield was obtained at pH 3-4. These characteristic profiles of the pigment formation were similar to those from alkadienals and essentially different from those from malonaldehyde. Alkadienals were likely candidates for the TBA-reactive substances in the homogenate.
Kikugawa
K
K
Tokyo College of Pharmacy, Japan.
Kojima
T
T
Kosugi
H
H
D000447:Aldehydes / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D015227:Lipid Peroxidation; D008099:Liver / Q000032:analysis*; D008297:Male; D008722:Methods; D051381:Rats; D011919:Rats, Inbred Strains; D013858:Thiobarbiturates
D000447:Aldehydes; D007202:Indicators and Reagents; D013858:Thiobarbiturates; C029684:thiobarbituric acid