title: Immunoelectron microscopy of type X collagen: supramolecular forms within embryonic chick cartilage.
abstract: To determine the supramolecular forms in which avian type X collagen molecules assemble within the matrix of hypertrophic cartilage, we performed immunoelectron microscopy with colloidal gold-labeled monoclonal antibodies. In addition double-labeled analyses were performed for the molecule and type II collagen, employing two monoclonal antibodies attached to different size gold particles. Both in situ limb cartilages and the extracellular matrix of chondrocyte cultures were examined. We observed in both systems that the type X collagen is present in two forms. One is as fine filaments (less than 5 nm in diameter) within mats which are found predominantly in the pericellular matrix of the hypertrophic chondrocytes. The second form is in association with the fibrils (10-20 nm in diameter) which also react with the antibody for type II collagen. It seems that the filamentous mats represent a form in which the type X collagen is initially secreted from the cell. The type X associated with the striated fibrils most likely represents a secondary association of the molecule with preexisting type II/IX/XI fibrils. The data are consistent with our previously proposed hypothesis that type X collagen is involved in, and perhaps even "targets," certain matrix components for degradation and removal.
authors.affiliation: Department of Biochemistry, Rush Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612.
authors.forename: T M
authors.initials: TM
authors.lastname: Schmid
authors.forename: T F
authors.initials: TF
authors.lastname: Linsenmayer
mesh_terms: D000818:Animals; D002356:Cartilage / Q000648:ultrastructure*; D002478:Cells, Cultured; D002642:Chick Embryo; D003094:Collagen / Q000648:ultrastructure*; D007150:Immunohistochemistry; D008854:Microscopy, Electron
chemical_list: D003094:Collagen