title: Osmotic regulation of vasopressin and oxytocin release is rate sensitive in hypothalamoneurohypophysial explants.
abstract: Explants of the hypothalamoneurohypophysial system (HNS) from rats were maintained in perifusion culture and exposed to 15-18 mosmol/kgH2O changes in the osmolality of the culture medium achieved by increasing or decreasing the NaCl concentration. The rate of change in osmolality was either 0.14 +/- 0.01 mosmol/min (2%/h), 0.27 +/- 0.02 mosmol/min (5%/h), or 1.7 +/- 0.2 mosmol/min (30%/h). Vasopressin (VP) and oxytocin (OT) release into the culture medium was determined. Increased VP release was detected after all three rates of increase, but the peak response occurred sooner and in response to a smaller increment in osmolality during the 5% rate of increase compared with the 2% rate of increase. Peak VP release occurred during the first 10 min of the 30% pulse and was significantly greater than the response to the 2 and 5% rates (P less than 0.05). The increase in VP release was sustained throughout the 3 h of hypertonicity during the 2% pulse, but not during the 5 and 30% osmotic pulses. A significant decrease in VP release was observed on returning the osmolality to basal at both the 2 and 5% rate, but this inhibition was followed by a rebound in VP release that started during the decrease in osmolality and significantly exceeded basal VP release. A significant inhibition of VP release also was observed when explants were exposed to hypotonic pulses at the 2 and 5% rate. At both rates, the inhibition of VP release corresponded to a 5- to 7-mosmol/kgH2O decrease in osmolality.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Neurology, University of Rochester, School of Medicine and Dentistry, New York 14642.
authors.forename: C
authors.initials: C
authors.lastname: Yagil
authors.forename: C D
authors.initials: CD
authors.lastname: Sladek
mesh_terms: D000818:Animals; D046508:Culture Techniques; D007030:Hypothalamo-Hypophyseal System / Q000378:metabolism*; D007038:Hypotonic Solutions / Q000494:pharmacology; D008297:Male; D009994:Osmolar Concentration; D010121:Oxytocin / Q000378:metabolism*; D010904:Pituitary Gland, Posterior / Q000378:metabolism; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012462:Saline Solution, Hypertonic / Q000494:pharmacology; D014667:Vasopressins / Q000378:metabolism*; D014882:Water-Electrolyte Balance / Q000502:physiology*
chemical_list: D007038:Hypotonic Solutions; D012462:Saline Solution, Hypertonic; D014667:Vasopressins; D010121:Oxytocin; D011189:Potassium Chloride