[Enzyme immunoassay (ELISA) for detecting S. aureus enterotoxins B and C in milk].
Staphylococcus aureus enterotoxins B and C, were obtained from strains 243 and 361 respectively. An immunoenzymatic technique was standardized both quantitative and qualitatively. We were able to detect enterotoxin to nanogram level on extracts from artificially contaminated milk. This ELISA immunoenzymatic technique implemented by us under the operative conditions of our working environment, resulted to be easy to handle, consumes only a short time, has a low cost and great diagnostic sensitivity.
Suárez-Semour
M A
MA
Depto. de Microbiología, Facultad de Medicina, U.A.N.L.
González
G M
GM
D000818:Animals; D004311:Double-Blind Method; D004768:Enterotoxins / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D008892:Milk / Q000032:analysis*; D013211:Staphylococcus aureus
D004768:Enterotoxins; C031818:enterotoxin B, staphylococcal; C034959:enterotoxin C, staphylococcal
Inmunoensayo enzimático (ELISA) para la detección de enterotoxinas B y C de S. aureus en leches.