Developmental regulation of human fetal-to-adult globin gene switching in transgenic mice.
Transgenic mice containing a human fetal (gamma-) or adult (beta-) globin gene linked to the beta-globin gene locus activation region (LAR) express the gene throughout development. By contrast, transgenic mice containing LAR linked to both a fetal and an adult globin gene display the normal developmental switch from fetal to adult gene expression. This suggests that the human fetal-to-adult globin gene switch is controlled through a mutually exclusive interaction between LAR and either the gamma- or beta-globin gene, resulting in the expression of only one gene at any given moment.
Enver
T
T
Department of Medicine, University of Washington, Seattle 98195.
Raich
N
N
Ebens
A J
AJ
Papayannopoulou
T
T
Costantini
F
F
Stamatoyannopoulos
G
G
D000818:Animals; D015139:Blotting, Southern; D004274:DNA, Recombinant; D004920:Erythropoiesis; D005786:Gene Expression Regulation; D005865:Gestational Age; D005914:Globins / Q000235:genetics*; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic / Q000502:physiology; D012045:Regulatory Sequences, Nucleic Acid
D004274:DNA, Recombinant; D005914:Globins