Release of [hydroxyproline3]-kinins by tissue kallikreins of pig, rat and man.
To determine the susceptibility of kininogens containing the recently described [Hyp3]-bradykinin moiety to cleavage by tissue kallikreins, we have studied the release of [Hyp3]-kinins from heat inactivated human plasma by purified tissue kallikreins. Kallikreins from man and pig were employed and compared with purified rat urinary kallikrein which is known to have a different cleavage specificity. Kinins released were separated by a modified reversed phase HPLC method and quantitated by bioassay and radioimmunoassay. Human urinary kallikrein and hog tissue kallikreins released 85-90% of the total kinins as Lys-bradykinin and 10-15% as [Hyp3]-Lys-bradykinin. In contrast, rat urinary kallikrein released 77% as bradykinin, 22% as [Hyp3]-bradykinin and negligible amounts of [Hyp3]-Lys-bradykinin from the identical substrate source indicating that rat tissue kallikreins prefer the Lys-Arg-bond within both unhydroxylated and hydroxylated kininogens. Therefore, hydroxylation of human kininogens apparently does not affect their ability to serve as substrates for tissue kallikreins with different cleavage specificities.
Maier
M
M
Department of Medical Physiology, University of Vienna, Austria.
Reissert
G
G
Jerabek
I
I
Bergmann
I
I
Balaun
E
E
Lottspeich
F
F
Binder
B R
BR
D000818:Animals; D001681:Biological Assay; D001920:Bradykinin / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006358:Hot Temperature; D006801:Humans; D006900:Hydroxylation; D006909:Hydroxyproline / Q000378:metabolism*; D007609:Kallidin / Q000378:metabolism; D007610:Kallikreins / Q000378:metabolism* / Q000652:urine; D007705:Kinins / Q000378:metabolism* / Q000494:pharmacology; D010902:Pituitary Gland / Q000201:enzymology; D010949:Plasma; D011863:Radioimmunoassay; D051381:Rats; D013552:Swine; D014599:Uterus / Q000187:drug effects
D007705:Kinins; D007609:Kallidin; D007610:Kallikreins; D006909:Hydroxyproline; D001920:Bradykinin