Highly polymorphic minisatellite DNA probes. Further evaluation for individual identification and paternity testing.
Reliable and reproducible protocols have been developed for the routine DNA fingerprinting of individuals using the highly polymorphic minisatellite DNA probes 33.15 and 33.6. Comparison of DNA fingerprinting from 50 individuals has generated further data on the level of band sharing in the DNA fingerprints of unrelated individuals, as well as the number of bands scorable in individuals. These results are consistent with previous studies. The occurrence of mutant bands in offspring has been examined in over 100 families. Further support is presented for the Mendelian inheritance of minisatellite loci and for lack of significant allelism and linkage between different variable DNA fragments detected in a human DNA fingerprint.
Smith
J C
JC
ICI Diagnostics, Northwich, Cheshire, United Kingdom.
Newton
C R
CR
Alves
A
A
Anwar
R
R
Jenner
D
D
Markham
A F
AF
D000483:Alleles; D015342:DNA Probes; D004276:DNA, Satellite / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D009710:Nucleotide Mapping; D010334:Paternity; D010375:Pedigree; D011110:Polymorphism, Genetic
D015342:DNA Probes; D004276:DNA, Satellite