A new method for extracting DNA or RNA for polymerase chain reaction.
The use of glass powder suspension for the extraction of RNA or DNA was studied to simplify the procedures of polymerase chain reaction (PCR). Using this procedure, proviral DNA of human T-lymphotropic virus type-1 (HTLV-1) in the blood of an asymptomatic virus carrier and viral RNA of human immunodeficiency virus (HIV) in the blood of an AIDS patient were easily detected by PCR employing glass powder. The use of glass powder is a simple and highly efficient procedure for the extraction of DNA or RNA, and can be applied for routine PCR.
Yamada
O
O
Department of Virology, Tottori University School of Medicine, Yonago, Japan.
Matsumoto
T
T
Nakashima
M
M
Hagari
S
S
Kamahora
T
T
Ueyama
H
H
Kishi
Y
Y
Uemura
H
H
Kurimura
T
T
D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis; D001483:Base Sequence; D015342:DNA Probes; D004279:DNA, Viral / Q000302:isolation & purification*; D005898:Glass; D006678:HIV / Q000302:isolation & purification; D015490:HTLV-I Infections / Q000175:diagnosis; D015368:Human T-lymphotropic virus 1 / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D011208:Powders; D011533:Proviruses / Q000302:isolation & purification; D012367:RNA, Viral / Q000302:isolation & purification*; D014773:Virology / Q000379:methods
D015342:DNA Probes; D004279:DNA, Viral; D011208:Powders; D012367:RNA, Viral