Detection of a novel minisatellite-specific DNA-binding protein.
We describe the detection of a ubiquitous DNA-binding protein which appears to interact specifically with tandem-repeated minisatellites. The murine 40 kd protein, which we term Msbp-1, was found to be present in all mouse tissues tested. This protein was bound specifically and with high affinity by double-stranded DNA containing a repeat sequence related to the minisatellite 'core' sequence, and binding required the presence of multiple repeat units. Corresponding minisatellite-specific DNA-binding proteins could also be detected in species ranging from Drosophila to man. This analysis represents the first direct evidence that minisatellites can function as a specific recognition signal for an endogenous DNA-binding protein.
Collick
A
A
Department of Genetics, University of Leicester, UK.
Jeffreys
A J
AJ
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D001667:Binding, Competitive; D001923:Brain Chemistry; D002467:Cell Nucleus / Q000032:analysis; D004276:DNA, Satellite / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000378:metabolism; D007668:Kidney / Q000032:analysis; D007700:Kinetics; D008099:Liver / Q000032:analysis; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009206:Myocardium / Q000032:analysis; D012091:Repetitive Sequences, Nucleic Acid; D013737:Testis / Q000032:analysis / Q000648:ultrastructure; D014018:Tissue Distribution
D004276:DNA, Satellite; D004268:DNA-Binding Proteins