Detection and quantitative determination of 3-nitropropionic acid in bovine urine.
The separation and determination of 3-nitropropionic acid (NPA) in bovine urine by reverse-phase HPLC is described. The method utilizes a rapid ethyl acetate extraction and this clean-up step also improves the chromatographic resolution of NPA in bovine plasma. A simple colorimetric method is also developed for the rapid detection and estimation of NPA in urine and the data indicate that it is in good agreement with the HPLC procedure.
Majak
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Research Station, Agriculture Canada, Kamloops, BC.
McDiarmid
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D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D009574:Nitro Compounds; D011422:Propionates / Q000097:blood / Q000652:urine*; D013053:Spectrophotometry; D013696:Temperature
D009574:Nitro Compounds; D011422:Propionates; C015392:3-nitropropionic acid