title: Evidence that the level of the p55 component of the interleukin (IL) 2 receptor can control IL 2 responsiveness in a murine IL 3-dependent cell.
abstract: Although the role of interleukin 2 (IL 2) in mature T lymphocyte function is well documented, its effect on hematopoietic progenitor cells is less well characterized. Here we have used recombinant retroviruses to transduce and express a cDNA clone encoding the p55 component of the human IL 2 receptor (h-p55), in a murine IL 3-dependent cell line, BAF3. While the parental cells do not respond to IL 2, the h-p55-expressing cells proliferate upon treatment with recombinant IL 2 after an initial lag period. The responsiveness of individual cell clones is correlated with their level of h-p55 expression, and can be inhibited by Tac monoclonal antibody. Furthermore, growth at limiting IL 2 concentrations selects a subset of cells expressing higher h-p55 levels from a bulk population. Detailed 125I-labeled IL 2 binding analysis on the highest h-p55-expressing clone detects the presence of 200 high-affinity (KD = 25 pM) IL 2 receptors. We therefore propose that the level of h-p55 expression governs the formation of high-affinity receptors, and hence IL 2 responsiveness in BAF3 cells.
authors.affiliation: Whitehead Institute, Cambridge, London, GB.
authors.forename: M K
authors.initials: MK
authors.lastname: Collins
authors.forename: P
authors.initials: P
authors.lastname: Malde
authors.forename: A
authors.initials: A
authors.lastname: Miyajima
authors.forename: K
authors.initials: K
authors.lastname: Arai
authors.forename: K A
authors.initials: KA
authors.lastname: Smith
authors.forename: R C
authors.initials: RC
authors.lastname: Mulligan
mesh_terms: D000818:Animals; D002455:Cell Division; D002460:Cell Line; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000502:physiology*; D007377:Interleukin-3 / Q000502:physiology; D008214:Lymphocytes / Q000502:physiology*; D046911:Macromolecular Substances; D051379:Mice; D008970:Molecular Weight; D015375:Receptors, Interleukin-2 / Q000502:physiology*; D011994:Recombinant Proteins; D014162:Transfection
chemical_list: D007376:Interleukin-2; D007377:Interleukin-3; D046911:Macromolecular Substances; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins