[Morphofunctional changes in potential liver allografts in experimental conditions].
As a part of morphofunctional investigations of validations cadaveric grafts for transplantation evolution of the histomorphological changes was followed by the experiments on guinea pigs. Liver samples were immediately after sacrificing the animals and 1, 2, and 4h later and kept at body temperature, which corresponded to the conditions of "warm ischemia". Second group of samples was immerged in Saline are +4 degrees C (hibernation-preservation) and was taken for analysis in same intervals as previous ones. Evolution of morphological and enzymatic changes was evident in short interval after sacrificing the animals. First changes were observed in cytoplasm and then in nuclei and interestitum. Changes of hepatic cells from hibernation preservation group were significant damaged comparing with the corresponding samples obtained from cadaver. ("warm ischemia"). These preliminary results indicated that length of liver graft preservation is very limited. Furthermore, this results demand for the future investigations in different conditions of preservation.
Popović
D
D
Simić
M
M
D000818:Animals; D006168:Guinea Pigs; D006801:Humans; D008099:Liver / Q000473:pathology*; D016031:Liver Transplantation; D009926:Organ Preservation; D013696:Temperature
Morfofunkcionalne promene potencijalnog algorafta jetre u eksperimentalnim uslovima.