title: The synthesis of polyamide-oligonucleotide conjugate molecules.
abstract: We have developed methods for the synthesis of peptide-oligodeoxyribonucleotide conjugate molecules in particular, and polyamide-oligonucleotide conjugates in general. Synthesis is carried out by a solid-phase procedure and involves the assembly of a polyamide on the solid support, conversion of the terminal amino group to a protected primary aliphatic hydroxy group by reaction with alpha, omega-hydroxycarboxylic acid derivatives, and finally oligonucleotide synthesis using phosphoramidite chemistry. The conjugate molecules can be used as DNA probes, with the polyamide component carrying one or more non-radioactive markers. These conjugates also have the potential to be used as anti-sense inhibitors of gene expression, with the peptide segment acting as a targeting moiety.
authors.affiliation: Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: J
authors.initials: J
authors.lastname: Haralambidis
authors.forename: L
authors.initials: L
authors.lastname: Duncan
authors.forename: K
authors.initials: K
authors.lastname: Angus
authors.forename: G W
authors.initials: GW
authors.lastname: Tregear
mesh_terms: D000595:Amino Acid Sequence; D015119:Aminocaproic Acid; D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D015342:DNA Probes / Q000138:chemical synthesis; D006880:Hydroxy Acids; D008969:Molecular Sequence Data; D009757:Nylons / Q000138:chemical synthesis*; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*
chemical_list: D015342:DNA Probes; D006880:Hydroxy Acids; D009757:Nylons; D009838:Oligodeoxyribonucleotides; D015119:Aminocaproic Acid