Immunocytochemical localization of rabbit gastric lipase and pepsinogen.
Lipase and pepsin activities were determined in rabbit gastric biopsy specimens. Lipase activity was found to be restricted to a small part of the fundic mucosa, near the cardia, whereas pepsin activity spread over about two thirds of the total fundic area, overlapping that of lipase. The cells producing these two enzymes were labeled by immunofluorescence using polyclonal antibodies against rabbit gastric lipase (RGL) or antibodies against rabbit pepsinogen. The immunocytochemical localization showed unequivocally that RGL and pepsinogen, which were both present in the cardial area, were in fact located in different gastric cells. The cells producing pepsinogen were in the lower base of the gastric fundic glands, whereas the cells producing RGL were in the upper base of the same glands. The cells producing pepsinogen and RGL showed no significant morphological differences. In the part of the fundic area, where only pepsin activity was detected, cells producing pepsinogen covered both the lower and the upper base of the gastric glands. No chief cells were observed in the antral mucosa. RGL and pepsinogen could represent useful gastric enzyme markers for cellular differentiation studies.
Moreau
H
H
Centre National de la Recherche Scientifique, Centre de Biochemie et de Biologie Moléculaire, Marseille/France.
Bernadac
A
A
Trétout
N
N
Gargouri
Y
Y
Ferrato
F
F
Verger
R
R
D000818:Animals; D015151:Immunoblotting; D007150:Immunohistochemistry; D008049:Lipase / Q000378:metabolism*; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D010435:Pepsinogens / Q000378:metabolism*; D011817:Rabbits; D013270:Stomach / Q000201:enzymology* / Q000648:ultrastructure
D010435:Pepsinogens; D008049:Lipase