Biophysical investigations on L1210 mouse leukemia cells treated with activated cyclophosphamide metabolites: determination of selective properties of DNA-interstrand and DNA-protein cross-linking mechanisms (alkaline elution and 31P-NMR-spectroscopy) and some aspects of the combination drug and radiotherapy.
Dose-effect relationships have been studied by using L1210 mouse leukemia cells treated with the activated cyclophosphamide derivatives 4-hydroperoxy-cyclophosphamide and 4-sulfido-cyclophosphamide. The combined treatment of the cells with the drugs and irradiation with X-rays indicates synergistic effects, if the irradiation of the cells is performed four to six hours after the drug treatment. DNA-interstrand and DNA-protein cross-linkings have been determined by an alkaline elution procedure. The yield of cross-linkings has been monitoring by either the 3H-14C method or by a 31P-NMR Fourier spectroscopy analysis of the eluted substrates.
Ulmer
W
W
FB Radiologie, Abteilung Medizinphysik/Biophysik, St.-Marien-Krankenhaus, Siegen.
D000818:Animals; D003131:Combined Modality Therapy; D003520:Cyclophosphamide / Q000008:administration & dosage / Q000378:metabolism*; D004247:DNA / Q000032:analysis; D004273:DNA, Neoplasm / Q000032:analysis* / Q000187:drug effects / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D005583:Fourier Analysis; D007939:Leukemia L1210 / Q000188:drug therapy / Q000532:radiotherapy / Q000628:therapy*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D011879:Radiotherapy Dosage; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D003520:Cyclophosphamide; D004247:DNA