2-Deoxy-D-glucose uptake in cultured human muscle cells.
Hexose uptake was studied with cultured human muscle cells using 2-deoxy-D-[1-3H]glucose. At a concentration of 0.25 and 4 mM, phosphorylation rather than transport was the rate-limiting step in the uptake of 2-deoxy-D-glucose. This was not due to inhibition of the hexokinase activity by either ATP depletion or 2-deoxyglucose 6-phosphate accumulation. In cellular homogenates, hexokinase showed a lower Km value for glucose as compared to 2-deoxyglucose. Intact cells preferentially phosphorylated glucose instead of 2-deoxyglucose. Therefore, transport instead of phosphorylation may be rate limiting in the uptake of glucose by cultured human muscle cells. These data suggest caution in using 2-deoxyglucose for measuring glucose transport.
Jacobs
A E
AE
Department of Biochemistry, University of Nijmegen, The Netherlands.
Oosterhof
A
A
Veerkamp
J H
JH
D019325:3-O-Methylglucose; D000227:Adenine Nucleotides / Q000032:analysis; D001692:Biological Transport; D002478:Cells, Cultured; D003837:Deoxy Sugars / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism* / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D019298:Glucose-6-Phosphate / Q000031:analogs & derivatives*; D005958:Glucosephosphates / Q000378:metabolism; D006019:Glycolysis; D006593:Hexokinase / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D007773:Lactates / Q000032:analysis; D008757:Methylglucosides / Q000378:metabolism; D009132:Muscles / Q000378:metabolism*; D010766:Phosphorylation
D000227:Adenine Nucleotides; D003837:Deoxy Sugars; D005958:Glucosephosphates; D007773:Lactates; D008757:Methylglucosides; D019325:3-O-Methylglucose; C015785:2-deoxyglucose-6-phosphate; D019298:Glucose-6-Phosphate; D003847:Deoxyglucose; D006593:Hexokinase; D005947:Glucose