Highly sensitive microplate-ELISA for angiotensin I using 3,3', 5,5'-tetramethylbenzidine.
A microplate-ELISA was established for angiotensin (Ang) I using Ang I-peroxidase conjugate, antiAng I antibodies which could distinguish Ang I from Ang II and Ang III, and 3,3', 5,5'-tetramethylbenzidine. Ang I could be quantified in amounts as low as 1 pg by the spectrophotometric method. The present assay system was the most sensitive and rapid in all Ang I assay systems described so far. The assay system was applicable to determination of Ang I concentration in human plasma and abdominal fluid.
Suzuki
F
F
Department of Biotechnology, Faculty of Agriculture, Gifu University, Japan.
Yamashita
S
S
Takahashi
A
A
Ito
M
M
Miyazaki
S
S
Nagata
Y
Y
Nakamura
Y
Y
D000328:Adult; D000803:Angiotensin I / Q000097:blood*; D001560:Benzidines; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008875:Middle Aged; D012084:Renin-Angiotensin System
D001560:Benzidines; C021758:3,3',5,5'-tetramethylbenzidine; D000803:Angiotensin I