Sequence characterization of the membrane protein gene of paramyxovirus simian virus 5.
The complete nucleotide sequence of the membrane (M) protein gene of the paramyxovirus simian virus 5 (SV5) was determined from cDNA clones of viral mRNAs. The M gene boundaries were determined by (i) primer extension sequencing on M mRNA; (ii) nuclease S1 analysis; and (iii) primer extension sequencing on viral genomic RNA. The M gene mRNA consisted of 1371 templated nucleotides. It contains a single large open reading frame that can encode a protein of 377 amino acids with a predicted Mr = 42,253. The authenticity of the predicted M protein coding sequence was confirmed by synthesis of the M protein from mRNA synthesized from cDNA. The predicted M amino acid sequence indicated it is an overall hydrophobic protein carrying a net positive charge. Alignment of the SV5 protein amino acid sequence with the M protein sequences of other paramyxoviruses indicated that these viruses fall into the following two groups: (1) SV5, mumps virus, and Newcastle disease virus; or (2) Sendai, parainfluenza virus type 3, measles virus, and canine distemper virus, with mumps virus M sequence being the most closely related to SV5.
Sheshberadaran
H
H
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208-3500.
Lamb
R A
RA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis; D001330:Electronic Data Processing; D005814:Genes, Viral; D006023:Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010252:Paramyxoviridae / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D014763:Viral Matrix Proteins / Q000235:genetics*
D004279:DNA, Viral; D006023:Glycoproteins; D012333:RNA, Messenger; D014763:Viral Matrix Proteins; C023587:protein M (glycoprotein)