Monoclonal antibodies directed against a synthetic peptide corresponding to the alpha-bungarotoxin binding region of the acetylcholine receptor.
Murine monoclonal antibodies have been produced against a 32 amino acid synthetic peptide corresponding to residues 173-204 on the alpha-subunit of the nicotinic acetylcholine receptor from Torpedo californica. All of the monoclonal antibodies were of the IgM subtype and most cross-reacted with the purified native receptor. None of the antibodies were effective in blocking alpha-bungarotoxin binding to the receptor nor, conversely, did alpha-bungarotoxin interfere with antibody binding. However, two monoclonal antibodies, previously shown to bind near the ligand binding site on the native receptor, did compete partially (50%) with the binding of one of the IgM monoclonal antibodies.
Preston-Hurlburt
P
P
Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06510.
Wilson
P T
PT
Dowding
A J
AJ
Hawrot
E
E
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology* / Q000378:metabolism; D001665:Binding Sites / Q000276:immunology; D001667:Binding, Competitive; D002038:Bungarotoxins / Q000378:metabolism*; D003429:Cross Reactions; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D011978:Receptors, Nicotinic / Q000276:immunology* / Q000378:metabolism; D014101:Torpedo / Q000378:metabolism
D000911:Antibodies, Monoclonal; D002038:Bungarotoxins; D007075:Immunoglobulin M; D010455:Peptides; D011978:Receptors, Nicotinic