Cloning and nucleotide sequence analysis of the human beta-microseminoprotein gene.
Beta-microseminoprotein (MSP) is a small protein (94 amino acids) synthesized by the epithelial cells of the prostate gland and secreted into the seminal plasma. Restriction endonuclease mapping of human genomic DNA with a human MSP cDNA probe identified a 19 kilobase (Kb) hybridizing band in both EcoRI and BamHI digestions. Subsequently, the 19 Kb EcoRI fragment of human genomic DNA containing the MSP gene was isolated and cloned into an EMBL4 phage vector. Screening of the recombinant phages resulted in the isolation of one clone containing the MSP gene. Restriction endonuclease mapping and sequence analysis of this clone revealed the human MSP gene of approximately 15 Kb in length. The gene contains four exons and three large introns of approximately 6, 1, and 7 Kb.
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Department of Biochemistry & Molecular Biology, University of Kansas Medical Center, Kansas City 66103.
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Kwok
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D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000302:isolation & purification; D005796:Genes; D006801:Humans; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000235:genetics*; D015183:Restriction Mapping; D012661:Semen / Q000378:metabolism; D029589:Seminal Plasma Proteins; D013735:Testicular Hormones / Q000235:genetics*
D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; D013735:Testicular Hormones; C043076:beta-microseminoprotein; D004247:DNA