Biotransformation of mafosfamide in P388 mice leukemia cells: intracellular 31P-NMR studies.
The intracellular transformation of cis-mafosfamide has been studied in P388 mice leukemia cells using 31P-NMR spectroscopy. For this purpose the cells were entrapped in low-gelling-temperature agarose threads. Internal pH of the cells, determined from the position of the intracellular inorganic phosphate, was 7.2. The cell membrane was permeable to 4-hydroxycyclophosphamide and aldophosphamide and less permeable to phosphoramide mustard. 4-Ketocyclophosphamide and carboxyphosphamide signals were not detectable in cells either sensitive or resistant to oxazaphosphorine treatment.
Sonawat
H M
HM
Fachbereich Chemie/Biologie, University of Brémen, F.R.G.
Leibfritz
D
D
Engel
J
J
Hilgard
P
P
D000818:Animals; D000970:Antineoplastic Agents / Q000378:metabolism*; D001711:Biotransformation; D003520:Cyclophosphamide / Q000031:analogs & derivatives* / Q000378:metabolism; D007941:Leukemia P388 / Q000378:metabolism*; D007942:Leukemia, Experimental / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D051379:Mice; D010758:Phosphorus; D014407:Tumor Cells, Cultured / Q000378:metabolism
D000970:Antineoplastic Agents; D010758:Phosphorus; C048341:mafosfamide; D003520:Cyclophosphamide