Protein differences in human pancreatic cancer cell lines with diverse metastatic potential.
High-resolution two-dimensional polyacrylamide gel electrophoresis was performed on the pancreatic cell lines SG, SG-R, FG, and L3.5, which when injected into the spleen of nude mice produced hepatic metastases in 0%, 20%, 64%, and 100% of the animals, respectively. A total of 981 proteins were quantitatively identified. In the highly metastatic lines, 13 proteins were present in statistically significant greater quantities, while 4 proteins were present in statistically significant greater quantities in the cell lines with a low metastatic potential. Two proteins were unique to the highly metastatic lines, while 16 proteins were unique to the lines with a low metastatic potential. These results suggest that there are considerable quantitative and qualitative differences in the cellular proteins of human pancreatic cancer cell lines with a varying metastatic potential and imply a biochemical basis to tumor heterogeneity and metastases.
Schwalke
M A
MA
Department of Surgery, Brown University, Providence, RI.
Doremus
C M
CM
Bleday
R
R
Wanebo
H J
HJ
Vezeridis
M P
MP
D000818:Animals; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008970:Molecular Weight; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000378:metabolism*; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000378:metabolism
D009363:Neoplasm Proteins