Deoxyribonucleic acid flow cytometric analysis of cervical adenocarcinoma: prognostic significance of deoxyribonucleic acid ploidy and S-phase fraction.
Paraffin-embedded tumor samples from 125 patients with cervical adenocarcinoma were analyzed by deoxyribonucleic acid flow cytometry. Thirty-one percent of the tumors were aneuploid. Triploid deoxyribonucleic acid content predominated (51.3%) and one third of the deoxyribonucleic acid aneuploid tumors were tetraploid, whereas near-diploid deoxyribonucleic acid aneuploidy was seen infrequently. Deoxyribonucleic acid aneuploidy was associated with tumor size, histologic grade, clinical stage, and high S-phase fraction. Deoxyribonucleic acid ploidy and S-phase fraction value were independent prognostic parameters, together with the presence of lymph node metastases and tumor size. In conclusion, our results indicate that flow cytometric deoxyribonucleic acid analysis helps to predict the prognosis and may thus influence the choice of treatment.
Leminen
A
A
Departments of Obstetrics and Gynecology, Helsinki University Central Hospital, Finland.
Paavonen
J
J
Vesterinen
E
E
Forss
M
M
Wahlström
T
T
Kulomaa
P
P
Lehtinen
M
M
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000328:Adult; D000368:Aged; D004247:DNA / Q000032:analysis* / Q000235:genetics; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007399:Interphase; D008875:Middle Aged; D011003:Ploidies; D011379:Prognosis; D012044:Regression Analysis; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology
D004247:DNA