title: Epileptiform activity in hippocampal slice cultures with normal inhibitory synaptic drive.
abstract: The synaptic events responsible for epileptiform burst discharge are often difficult to define. Blockade of inhibition has been used to produce epileptiform events, but it is unclear whether increased excitatory activity in the presence of normal inhibition can also result in burst discharge. In the hippocampal slice culture preparation, a small percentage of cultures exhibit spontaneous bursts. To determine whether the absence of inhibitory postsynaptic potentials (IPSPs) is responsible for these spontaneous bursts, we applied the glutamate antagonist, kynurenic acid (KYN) to block burst activity, and unmask any underlying IPSPs. KYN (10 mM) quickly reduced synaptic activity with concomitant loss of burst discharge. Washout of KYN resulted in a gradual return of synaptic activity, during which time both fast and slow IPSPs were clearly observed. As burst activity returned to control levels, excitatory postsynaptic potentials (EPSPs) were increasingly superimposed within the inhibitory events, obscuring (but not eliminating) the IPSPs. In these hippocampal slice cultures, therefore, epileptiform bursts appear to be the result of an abnormally high level of excitatory synaptic drive, not a reduction in inhibition.
authors.affiliation: Department of Neurological Surgery, University of Washington, Seattle 98195.
authors.forename: A T
authors.initials: AT
authors.lastname: Malouf
authors.forename: C A
authors.initials: CA
authors.lastname: Robbins
authors.forename: P A
authors.initials: PA
authors.lastname: Schwartzkroin
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D004827:Epilepsy / Q000503:physiopathology*; D006624:Hippocampus / Q000187:drug effects / Q000503:physiopathology*; D066298:In Vitro Techniques; D007736:Kynurenic Acid / Q000494:pharmacology; D009433:Neural Inhibition; D051381:Rats
chemical_list: D007736:Kynurenic Acid