title: Immunogenic integral membrane proteins of Borrelia burgdorferi are lipoproteins.
abstract: The pathogenic spirochete Borrelia burgdorferi contains a set of integral membrane proteins which were selectively extracted into the detergent phase upon solubilization of intact B. burgdorferi with the nonionic detergent Triton X-114. Virtually all of these hydrophobic proteins were recognized by antibodies in pooled sera from patients with chronic Lyme arthritis, demonstrating that proteins partitioning into the detergent phase of Triton X-114 encompass the major B. burgdorferi immunogens. Furthermore, most of these immunogenic proteins, including the previously characterized OspA and OspB membrane antigens, could be biosynthetically labeled when B. burgdorferi was incubated in vitro with [3H]palmitate. The OspA and OspB antigens were radioimmunoprecipitated from [3H]palmitate-labeled detergent-phase proteins with monoclonal antibodies, and [3H]palmitate was recovered unaltered from these proteins after sequential alkaline and acid hydrolyses. The combined results provide formal confirmation that the major B. burgdorferi immunogens extracted by Triton X-114 are lipoproteins. The demonstration that B. burgdorferi integral membrane antigens are lipoproteins may explain the basis of their immunogenicity and may help to improve our understanding of the surface topology of B. burgdorferi.
authors.affiliation: Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235.
authors.forename: M E
authors.initials: ME
authors.lastname: Brandt
authors.forename: B S
authors.initials: BS
authors.lastname: Riley
authors.forename: J D
authors.initials: JD
authors.lastname: Radolf
authors.forename: M V
authors.initials: MV
authors.lastname: Norgard
mesh_terms: D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D001426:Bacterial Proteins / Q000032:analysis* / Q000276:immunology; D015748:Borrelia burgdorferi Group / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis; D015151:Immunoblotting; D008074:Lipoproteins / Q000032:analysis* / Q000276:immunology; D008565:Membrane Proteins / Q000032:analysis* / Q000276:immunology; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D005227:Fatty Acids; D008074:Lipoproteins; D008565:Membrane Proteins; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40