Rapid increase in inositol pentakisphosphate accumulation by nicotine in cultured adrenal chromaffin cells.
When [3H]inositol-prelabeled cultured bovine adrenal chromaffin cells were stimulated with nicotine (10 microM), a large and transient increase in [3H]inositol pentakisphosphate (InsP5) accumulation was observed. The accumulation reached the maximum level at 15 s, then declined to the basal level at 2 min. Nicotine also induced [3H]inositol tetrakisphosphate (InsP4) and [3H]inositol hexakisphosphate (InsP6) accumulation with a slower time course and a lesser magnitude than [3H]InsP5. The peaks of [3H]InsP4, [3H]InsP5 and [3H]InsP6 coincided with those of 32P radioactivity, when cells were doubly labeled with [3H]inositol and inorganic 32P. These results suggest that inositol pentakisphosphate is rapidly increased by nicotine, a cholinergic agonist, in cultured adrenal chromaffin cells.
Sasakawa
N
N
Department of Pharmacology, Keio University School of Medicine, Tokyo, Japan.
Nakaki
T
T
Kato
R
R
D000313:Adrenal Medulla / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D002838:Chromaffin System / Q000187:drug effects / Q000378:metabolism*; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D007295:Inositol Phosphates / Q000378:metabolism*; D007700:Kinetics; D009538:Nicotine / Q000494:pharmacology*; D010833:Phytic Acid / Q000378:metabolism
D007295:Inositol Phosphates; C013571:inositol pentaphosphate; D009538:Nicotine; D010833:Phytic Acid; D015544:Inositol 1,4,5-Trisphosphate