Analysis of factors influencing clonogenic growth in vitro of cells from ovarian carcinoma patients.
Clonogenic growth (defined as the formation of greater than or equal to 5 colonies per 5 x 10(5) viable nucleated cells per plate) of ovarian cancer specimens assessed in our clonogenic assay system was significantly associated with the proportion of tumor cells in the suspensions plated (N = 87; P = 0.0006), although there was no quantitative relationship with the corresponding plating efficiencies. An inverse correlation was observed between monocytes/macrophages/mesothelial cells (M) proportion and clonogenic growth (P = 0.013). These associations were most evident when only effusions were considered. Univariate analyses identified tumor cell content, M proportion and, to a lesser degree, granulocyte content as the only factors out of 12 examined to be correlated with colony formation. Multivariate analysis using a logistic regression model identified the proportion of tumor cells as the only significant factor predicting clonogenic growth in vitro (P = 0.0006). The overall accuracy of prediction for growth or non-growth was 63.2%.
Dittrich
C
C
Department of Chemotherapy, University of Vienna, Austria.
Dittrich
E
E
Wrba
F
F
Hudec
M
M
Grunt
T
T
Salzer
H
H
Sevelda
P
P
Eliason
J
J
D000362:Agar; D000704:Analysis of Variance; D002452:Cell Count; D002455:Cell Division; D002498:Centrifugation; D005260:Female; D006098:Granulocytes; D006801:Humans; D066298:In Vitro Techniques; D014411:Neoplastic Stem Cells / Q000166:cytology*; D010051:Ovarian Neoplasms / Q000473:pathology*; D010586:Phagocytes; D011336:Probability; D012044:Regression Analysis; D014410:Tumor Stem Cell Assay
D000362:Agar