title: Multiple fluorescence in situ hybridization.
abstract: A method for multiple fluorescence in situ hybridization is described allowing the simultaneous detection of more than three target sequences with only three fluorescent dyes (FITC, TRITC, AMCA), respectively emitting in the green, red, and blue. This procedure is based on the labeling of (DNA) probes with more than one hapten and visualisation in multiple colors. The possibility to detect multiple targets simultaneously is important for prenatal diagnosis and the detection of numerical and/or structural chromosome aberrations in tumor diagnosis. It may form the basis for an in situ hybridization based chromosome banding technique.
authors.affiliation: Department of Cytochemistry and Cytometry, University of Leiden, The Netherlands.
authors.forename: P M
authors.initials: PM
authors.lastname: Nederlof
authors.forename: S
authors.initials: S
authors.lastname: van der Flier
authors.forename: J
authors.initials: J
authors.lastname: Wiegant
authors.forename: A K
authors.initials: AK
authors.lastname: Raap
authors.forename: H J
authors.initials: HJ
authors.lastname: Tanke
authors.forename: J S
authors.initials: JS
authors.lastname: Ploem
authors.forename: M
authors.initials: M
authors.lastname: van der Ploeg
mesh_terms: D015073:2-Acetylaminofluorene; D001710:Biotin / Q000031:analogs & derivatives; D002875:Chromosomes / Q000032:analysis*; D002878:Chromosomes, Human, Pair 1 / Q000032:analysis; D003582:Cytogenetics / Q000379:methods*; D015342:DNA Probes; D003856:Deoxyuracil Nucleotides; D005456:Fluorescent Dyes; D006801:Humans; D008297:Male; D008856:Microscopy, Fluorescence; D009693:Nucleic Acid Hybridization; D012815:Signal Processing, Computer-Assisted
chemical_list: D015342:DNA Probes; D003856:Deoxyuracil Nucleotides; D005456:Fluorescent Dyes; D001710:Biotin; C045931:biotin-11-dUTP; D015073:2-Acetylaminofluorene