Biliary lipid secretion during the prereplicative phase of rat liver regeneration.
The prereplicative phase-related changes in spontaneous and taurocholate-induced biliary lipid secretion were studied in anaesthetized male Wistar rats (250 g). Rats underwent two-thirds hepatectomy 1, 6 or 12 h before starting to collect bile samples. As compared with non-hepatectomized rats, biliary lipid secretion was increased at 1 h after hepatectomy and then restored to values similar to the control group up to 12 h after hepatectomy. In separate experiments, taurocholate was infused (200 nmol/min/g calculated liver weight) through the jugular vein over 80 min. Both taurocholate-induced bile flow and bile acid output were similar in control and hepatectomized rats, regardless of the time of the prereplicative phase considered. By contrast, taurocholate-induced lecithin and cholesterol outputs were markedly modified. The former was lowered throughout the prereplicative phase, whereas the latter increased at 6 h and decreased at 12 h. In summary, these results indicate that shortly after hepatectomy bile acid-induced biliary lipid secretion is profoundly modified, probably due to changes in the plasma membrane involved in preparing the hepatocyte to enter the cell cycle.
Perez-Barriocanal
F
F
Department of Physiology and Pharmacology, University of Salamanca, Spain.
Perez-Antona
J C
JC
Regueiro
P
P
Villanueva
G R
GR
Marin
J J
JJ
D000818:Animals; D001646:Bile / Q000187:drug effects / Q000378:metabolism*; D001647:Bile Acids and Salts / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism*; D008115:Liver Regeneration; D008297:Male; D010713:Phosphatidylcholines / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012636:Secretory Rate; D013656:Taurocholic Acid / Q000494:pharmacology
D001647:Bile Acids and Salts; D010713:Phosphatidylcholines; D013656:Taurocholic Acid; D002784:Cholesterol