Is there a circulating proteolysis-inducing factor during sepsis?
Muscles from fed or 72-hour fasted rats were incubated in the presence of plasma from septic rats, recombinant interleukin 1 alpha (rIL-1 alpha), or recombinant tumor necrosis factor alpha (rTNF alpha), and breakdown of total and myofibrillar protein was assessed by determining release of tyrosine and 3-methylhistidine, respectively. Septic plasma stimulated total protein breakdown in muscles from 72-hour fasted rats by 10% to 20%, while myofibrillar protein breakdown was not affected. When septic plasma was added to muscles from fed rats, neither tyrosine nor 3-methylhistidine release was altered. Various concentrations of recombinant interleukin 1 alpha or recombinant tumor necrosis factor alpha did not affect total or myofibrillar protein breakdown. Since septic plasma did not stimulate myofibrillar protein breakdown, the role of a circulating factor for muscle proteolysis during sepsis remains unclear.
Hasselgren
P O
PO
Department of Surgery, University of Cincinnati Medical Center, OH 45267-0558.
James
J H
JH
Benson
D W
DW
Li
S
S
Fischer
J E
JE
D000818:Animals; D003345:Corticosterone / Q000494:pharmacology; D006639:Histidine / Q000031:analogs & derivatives*; D066298:In Vitro Techniques; D007239:Infections / Q000097:blood* / Q000378:metabolism; D007375:Interleukin-1 / Q000494:pharmacology; D008297:Male; D008762:Methylhistidines / Q000378:metabolism*; D009119:Muscle Contraction; D009124:Muscle Proteins / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014443:Tyrosine / Q000378:metabolism*
D007375:Interleukin-1; D008762:Methylhistidines; D009124:Muscle Proteins; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D014443:Tyrosine; D006639:Histidine; C028118:3-methylhistidine; D003345:Corticosterone