Determination of Senecio alkaloids by thermospray liquid chromatography/mass spectrometry.
A series of Senecio alkaloid and alkaloid N-oxide standards has been analyzed using positive and negative ion thermospray liquid chromatography/mass spectrometry (LC/MS) with an ammonium acetate-containing mobile phase. On-line separations of pyrrolizidine alkaloids from extracts of Senecio jacobaea (tansy ragwort) and Senecio vulgaris (common groundsel) were done using an ammonium hydroxide-containing mobile phase. All of the alkaloids known to be present in the extracts were detected by ammonium hydroxide thermospray LC/MS, as well as many other components which may be as-yet-unidentified alkaloids.
Parker
C E
CE
Laboratory of Molecular Biophysics, NIEHS, Research Triangle Park, North Carolina 27709.
Verma
S
S
Tomer
K B
KB
Reed
R L
RL
Buhler
D R
DR
D000085:Acetates; D000470:Alkaloids / Q000032:analysis*; D064753:Ammonium Hydroxide; D002851:Chromatography, High Pressure Liquid; D006878:Hydroxides; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000032:analysis; D010947:Plants, Toxic; D011763:Pyrrolizidine Alkaloids / Q000032:analysis / Q000302:isolation & purification; D012674:Senecio / Q000032:analysis*; D012997:Solvents
D000085:Acetates; D000470:Alkaloids; D006878:Hydroxides; D010936:Plant Extracts; D011763:Pyrrolizidine Alkaloids; D012997:Solvents; D064753:Ammonium Hydroxide; C018824:ammonium acetate