Enhancement by L-methionine on the contractile response of uterine segments to acetylcholine and high KCl is mainly due to enhancement of Ca uptake.
Acetylcholine (ACh)- and high KCl-stimulated 45Ca uptake into rat uterine segments was inhibited by 3-deazaadenosine (3-DAA) plus homocysteine thiolactone (HCTL), and this inhibitory effect was attenuated by L-methionine (L-Met). Ca-depleted Ringer solution in which uterine muscle had been incubated with L-Met did not enhance the contractile response of another uterine segment to ACh and high KCl in the presence of 3-DAA plus HCTL. These findings together with previous results suggest that the enhancing effect of L-Met on the contractile responses to ACh and high KCl is mainly due to an increase in Ca2+ uptake into the uterine muscle.
Ichida
S
S
Department of Biological Chemistry, Faculty of Pharmacy, Kinki University, Higashi-Osaka, Japan.
Yoshioka
K
K
Ariyoshi
S
S
Fujisue
T
T
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D005260:Female; D006710:Homocysteine / Q000031:analogs & derivatives / Q000494:pharmacology; D066298:In Vitro Techniques; D008715:Methionine / Q000494:pharmacology*; D011189:Potassium Chloride / Q000494:pharmacology*; D011247:Pregnancy; D051381:Rats; D014372:Tubercidin / Q000494:pharmacology; D014590:Uterine Contraction / Q000187:drug effects*; D014599:Uterus / Q000187:drug effects / Q000378:metabolism
C018258:3-deazaadenosine; D006710:Homocysteine; D011189:Potassium Chloride; D008715:Methionine; C007957:homocysteine thiolactone; D014372:Tubercidin; D000109:Acetylcholine; D002118:Calcium