Supernatant protein and cellulase activities of the anaerobic ruminal fungus Neocallimastix frontalis EB188.
Protein and cellulose activities were measured in culture supernatants of the anaerobic ruminal fungus Neocallimastix frontalis EB188 established in glucose medium and switched to either glucose, cellobiose, or cellulose media. Polyacrylamide gel electrophoresis was used to show differences caused by changing medium carbon source. Culture supernatants contained proteins with molecular weights ranging from greater than 116,000 to about 19,000. Low levels of cellulose activity were evident in glucose-grown cultures. Increased amounts of slowly migrating cellulase activities appeared in the supernatants of glucose-grown cultures switched to cellulose. Cellulase activities which reacted differentially during colorimetric and in situ assays were produced. Isoelectric points of cellulase activities varied from 3.7 to 8.3, and activities possessed optimal pHs of between 5.9 and 6.5.
Barichievich
E M
EM
Department of Animal Sciencesm Washington State University, Pullman 99164-6332.
Calza
R E
RE
D000693:Anaerobiosis; D000818:Animals; D002417:Cattle; D002480:Cellulase / Q000096:biosynthesis*; D002482:Cellulose / Q000378:metabolism; D002850:Chromatography, Gel; D008411:Chytridiomycota / Q000201:enzymology / Q000378:metabolism*; D003124:Colorimetry; D003470:Culture Media; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000096:biosynthesis*; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D008970:Molecular Weight; D012417:Rumen / Q000382:microbiology*
D003470:Culture Media; D005656:Fungal Proteins; D002482:Cellulose; D002480:Cellulase; D005947:Glucose