The effect of taurine on age-related immune decline in mice: the effect of taurine on T cell and B cell proliferative response under costimulation with ionomycin and phorbol myristate acetate.
Proliferative responses to the costimulation with phorbol-12-myristate-13-acetate (PMA) and suboptimal doses of ionomycin in the purified T and B cells from old mice were lower than those from young mice. The degree of the age-related decline was more significant in T cells than in B cells. Taurine, a sulfur containing amino acid, augmented the proliferative responses of T cells from both young and old mice. The augmentation of the proliferative response by taurine was more marked in old T cells than in young ones. The concentration of intracellular free calcium ion ([Ca2+]i) was significantly lower in the old T cells under the stimulation with PMA and ionomycin than that in the young ones. In the presence of taurine, the concentration of [Ca2+]i in the old T cell significantly increased under the stimulation. The results indicate that taurine improved the proliferative response of old T cells by the restoration of the increment of the concentration of [Ca2+]i under the stimulation.
Nishio
S
S
Third Department of Internal Medicine, Osaka University Hospital, Japan.
Negoro
S
S
Hosokawa
T
T
Hara
H
H
Tanaka
T
T
Deguchi
Y
Y
Ling
J
J
Awata
N
N
Azuma
J
J
Aoike
A
A
D000375:Aging / Q000276:immunology*; D000818:Animals; D002118:Calcium / Q000493:pharmacokinetics / Q000502:physiology; D007107:Immune System / Q000502:physiology; D015759:Ionomycin / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013654:Taurine / Q000493:pharmacokinetics / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D013654:Taurine; D015759:Ionomycin; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium