Nonsense suppressors partially revert the decrease of the mRNA level of a nonsense mutant allele in yeast.
suc2 degrees is a naturally occurring amber mutant allele of the yeast invertase structural gene SUC2. Strains carrying suc2 degrees had only 10% of the wild type invertase-specific mRNA level. Amber suppressors, which allowed suc2 degree strains to ferment sucrose caused an increase of the SUC-specific mRNA level.
Gozalbo
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Institut für Mikrobiologie, Technische Hochschule Darmstadt, Federal Republic of Germany.
Hohmann
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D000483:Alleles; D015152:Blotting, Northern; D005800:Genes, Fungal; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D012331:RNA, Fungal / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis*; D013489:Suppression, Genetic; D014170:Transformation, Genetic; D015003:Yeasts / Q000201:enzymology / Q000235:genetics*; D043324:beta-Fructofuranosidase
D012331:RNA, Fungal; D012333:RNA, Messenger; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase