Crystallographic characterization of a Cu,Zn superoxide dismutase from Photobacterium leiognathi.
Crystals of a copper-zinc superoxide dismutase from Photobacterium leiognathi, a luminescent marine bacterium that is the species-specific symbiont of the ponyfish, have been obtained from 2-methyl-2,4-pentanediol solutions. The space group was determined using screenless small-angle precession photographs, and was confirmed by analyzing area detector diffraction data with the XENGEN programs for indexing and refinement. The crystals are monoclinic, space group C2 (a = 126.4 A, b = 87.0 A, c = 44.4 A, beta = 92.8 A), and have two 32,000 Mr dimers per asymmetric unit. The crystals diffract to at least 2.7 A resolution, are resistant to radiation damage, and are suitable for determination of the structure by X-ray diffraction.
Redford
S M
SM
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
McRee
D E
DE
Getzoff
E D
ED
Steinman
H M
HM
Tainer
J A
JA
D010776:Photobacterium / Q000201:enzymology*; D013482:Superoxide Dismutase; D014961:X-Ray Diffraction
D013482:Superoxide Dismutase