Separation and quantification by gas chromatography-mass spectrometry of arabinitol enantiomers to aid the differential diagnosis of disseminated candidiasis.
To differentiate increased arabinitol due to fungal (only D-arabinitol) and non-fungal origin, O-trifluoroacetyl derivatives of the enantiomers were separated using alpha-perpentylated cyclodextrin columns and measured by selected ion monitoring. Mean +/- S.D. D/L in normal serum: 1.40 +/- 0.42. D/L ratios greater than 2.24, defined as normal mean + 2S.D., were considered outside normal range. D/L was greater than 2.2 in 10 of 12 confirmed candidiasis cases with one false negative and one borderline. Renal dysfunction without candidiasis yielded normal D/L despite high arabinitol concentrations. D/L in normal urine was nearly identical to that in serum despite 60 times larger concentration. D/L ratios, determined by peak heights or areas, could be used without the need to determine concentrations.
Roboz
J
J
Department of Neoplastic Diseases, Mount Sinai School of Medicine, New York, NY 10029.
Nieves
E
E
Holland
J F
JF
D002177:Candidiasis / Q000097:blood / Q000175:diagnosis*; D003201:Computers; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D012016:Reference Values; D012255:Ribitol / Q000097:blood; D013237:Stereoisomerism; D013402:Sugar Alcohols / Q000097:blood* / Q000652:urine; D014993:Xylitol / Q000097:blood
D013402:Sugar Alcohols; D012255:Ribitol; D014993:Xylitol; C014999:arabitol