Retention of antimony in skin biopsies of leishmaniasis patients after treatment with N-methylglucamine antimoniate.
Leishmaniasis patients were treated with N-methylglucamine antimoniate by intravenous injections of 10 or 20 mg of Sb per kilogram of body weight per day for 10 or 20 days. Digests of skin biopsies taken from the site of lesion before and after treatment were analyzed for antimony by instrumental neutron activation (INAA). The detection limit of the assay was 20 ng, and no Sb could be measured in digests of samples (less than 2.45 mg) taken before treatment. Biopsies taken after injections of Sb showed concentrations in the range of 8.32 to 70.68 ng/g skin. We discuss the usefulness of INAA in the study of Sb in small samples of tissues.
Dorea
J G
JG
Division of Nutrition and Tropical Medicine, University of Brasilia, Brazil.
Merchan-Hamann
E
E
Ryan
D E
DE
Holzbecher
J
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D000965:Antimony / Q000032:analysis*; D001706:Biopsy; D002648:Child; D004311:Double-Blind Method; D004334:Drug Administration Schedule; D006801:Humans; D007275:Injections, Intravenous; D007896:Leishmaniasis / Q000188:drug therapy* / Q000378:metabolism; D008536:Meglumine / Q000008:administration & dosage / Q000627:therapeutic use*; D000077485:Meglumine Antimoniate; D008875:Middle Aged; D009501:Neutron Activation Analysis; D009942:Organometallic Compounds / Q000008:administration & dosage / Q000627:therapeutic use*; D011897:Random Allocation; D012867:Skin / Q000032:analysis / Q000378:metabolism*; D013012:Sorbitol / Q000031:analogs & derivatives*
D009942:Organometallic Compounds; D013012:Sorbitol; D008536:Meglumine; D000077485:Meglumine Antimoniate; D000965:Antimony