Immunohistochemistry of endogenous L-DOPA in the rat posterior hypothalamus.
The aim of this work was to study L-DOPA-containing neuronal structures of the rat posterior and dorsal hypothalamus by means of immunohistochemistry using antiserum against glutaraldehyde conjugated L-DOPA. Aspects and distribution of L-DOPA immunoreaction among cells of the supramammillary nucleus and the A11, A13c and A13 cell groups are described and compared to dopamine immunoreactivity, mainly through a double colored labelling procedure employing a color modification of the DAB reaction by metallic ions. Differences between L-DOPA and dopamine stainings within cell groups as the presence of cells with predominant or exclusive L-DOPA coloration are tentatively explained under the light of previous findings using immunohistochemistry of catecholamines synthesizing enzymes and catecholamines histofluorescence.
Tison
F
F
Département de Neurologie, Hôpital Pellegrin, Bordeaux, France.
Mons
N
N
Geffard
M
M
Henry
P
P
D000818:Animals; D004298:Dopamine / Q000032:analysis*; D007031:Hypothalamus / Q000032:analysis*; D007034:Hypothalamus, Posterior / Q000032:analysis* / Q000166:cytology; D007150:Immunohistochemistry; D007980:Levodopa / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D007980:Levodopa; D004298:Dopamine