Influence of cholesterol oxides on endocytosis of cultured endothelial and smooth muscle cells.
Human umbilical vein endothelial cells and rabbit aortic smooth muscle cells in culture were incubated for intervals up to 24 hrs with varying concentrations of cholesterol, 7-ketocholesterol, 25-hydroxycholesterol, cholestane-3 beta,5 alpha,6 beta-triol or cholesterol-5 alpha, 6 beta-epoxide. Endocytosis, as measured by uptake of horseradish peroxidase (HRP), was inhibited in a dose and time dependent manner in both endothelial and smooth muscle cell cultures by cholestane-3 beta,5 alpha,6 beta-triol and 25-hydroxycholesterol. Inhibition by 7-ketocholesterol in endothelial cells occurred only at higher concentrations, and cholesterol and cholesterol epoxide showed no significant inhibitory effects. The viability of the cells exposed to the cholesterol oxides at the concentrations that inhibited the uptake of HRP was not changed. Cholesterol oxides induce functional endothelial injury, not morphologically apparent, which may be involved in atherogenesis.
Peng
S K
SK
Department of Pathology, UCLA School of Medicine, Torrance 90509.
Hu
B
B
Hsiao
W
W
Morin
R
R
D000818:Animals; D001011:Aorta; D002478:Cells, Cultured; D002777:Cholestanols / Q000494:pharmacology; D002784:Cholesterol / Q000031:analogs & derivatives / Q000494:pharmacology*; D004705:Endocytosis / Q000187:drug effects*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology*; D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D006888:Hydroxycholesterols / Q000494:pharmacology; D000960:Hypolipidemic Agents; D007653:Ketocholesterols / Q000494:pharmacology; D007700:Kinetics; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology*; D011817:Rabbits; D014471:Umbilical Veins
D002777:Cholestanols; D006888:Hydroxycholesterols; D000960:Hypolipidemic Agents; D007653:Ketocholesterols; C000474:cholestane-3,5,6-triol; C002415:cholesterol alpha-oxide; C007997:25-hydroxycholesterol; D002784:Cholesterol; D006735:Horseradish Peroxidase; C003001:7-ketocholesterol