Characteristic immunophenotyping artefact seen in patients with anti-mouse immunoglobulin antibodies.
Patients treated with murine monoclonal antibodies develop anti-mouse immunoglobulin antibodies which can interfere with flow cytometry of lymphocytes, giving rise to high levels of nonspecific staining. This artefact can be avoided by using separated, washed lymphocytes for immunophenotyping.
Wilks
D
D
Retroviral Research Group, Clinical Research Centre, Harrow.
Byrom
N
N
Walker
L
L
Habeshaw
J
J
Dalgleish
A
A
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000627:therapeutic use; D015704:CD4 Antigens / Q000276:immunology*; D005434:Flow Cytometry; D015658:HIV Infections / Q000276:immunology / Q000628:therapy*; D006801:Humans; D007167:Immunotherapy; D051379:Mice; D010641:Phenotype
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D015704:CD4 Antigens