Molecular cloning, expression, and chromosome 19 localization of a human Ro/SS-A autoantigen.
Ro/SS-A antibodies are found in a number of human autoimmune disorders including Sjogren's syndrome and several systemic lupus erythematosus-related disorders. These heterogeneous autoantibodies are known to recognize several distinct cellular antigens. With synthetic oligonucleotides corresponding to amino acid sequence information we have isolated a full-length cDNA clone which encodes a human Ro ribonucleoprotein autoantigen. The 1,890-base pair clone contains an open reading frame that encodes a 417-amino acid, 48-kD polypeptide that migrates aberrantly at 60 kD by SDS-PAGE. Rabbit antibodies raised against this protein's recently described amino-terminal epitope react with a previously identified 52-kD human Ro protein and immunoprecipitate the human cytoplasmic RNAs. Ultraviolet light cross-linking studies suggest that this Ro protein binds each of the four major human cytoplasmic RNAs. The deduced amino acid sequence is 63% homologous to an Onchocerca volvulus antigen. Southern filter hybridization analysis indicates that this gene is not highly polymorphic and exists as a single copy in the human genome. Chromosomal localization studies place this gene on the short arm of chromosome 19 near the gene encoding the low density lipoprotein receptor.
McCauliffe
D P
DP
Department of Dermatology, University of Texas, Southwestern Medical Center, Dallas 75238.
Lux
F A
FA
Lieu
T S
TS
Sanz
I
I
Hanke
J
J
Newkirk
M M
MM
Bachinski
L L
LL
Itoh
Y
Y
Siciliano
M J
MJ
Reichlin
M
M
D000595:Amino Acid Sequence; D001324:Autoantigens / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002460:Cell Line; D002888:Chromosomes, Human, Pair 19; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D020733:RNA, Small Cytoplasmic; D015183:Restriction Mapping; D012261:Ribonucleoproteins; D012689:Sequence Homology, Nucleic Acid; D012984:Software; D000099207:SS-A Antigen
D001324:Autoantigens; D004247:DNA; D015345:Oligonucleotide Probes; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D000099207:SS-A Antigen; C116006:RO60 protein, human