title: Application of the immunohistochemical method as a predictive assay in radiotherapy of squamous cell carcinoma of oropharynx and hypopharynx.
abstract: We analyzed various subsets of lymphocytes infiltrated into cancer tissues from 15 patients (10 cases of oropharyngeal cancer and 5 of hypopharyngeal cancer) before treatment and after irradiation with approximately 10 Gy (1,000 rad) and 30 Gy by the method of biotin-avidin-horseradish peroxidase using mouse monoclonal antibodies. The infiltration was graded (marked) to - (none). In 3 cases, tumor-infiltrating lymphocytes were remarkably increased at the delivery of a small dose of irradiation (approx. 10 Gy): 2 of them showed remarkable radiosensitivity, and the lymphocyte subpopulation of the cancer tissue was mainly composed of Leu-3a + 3b-positive and Leu-8-negative lymphocytes (helper inducer T lymphocytes or delayed-type hypersensitivity cells). On the other hand, the third one proved to be rather radioresistant, and the lymphocyte subpopulation was mainly composed of Leu-14-positive lymphocytes (B lymphocytes). These findings indicate that the analysis of the lymphocyte subpopulation infiltrating into cancer tissues at the delivery of small doses of irradiation is applicable as a predictive assay in radiotherapy of squamous cell carcinoma of the oropharynx and hypopharynx.
authors.affiliation: Department of Radiology, Kochi Medical School, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ogawa
authors.forename: T
authors.initials: T
authors.lastname: Maeda
authors.forename: H
authors.initials: H
authors.lastname: Seguchi
authors.forename: T
authors.initials: T
authors.lastname: Inomata
authors.forename: S
authors.initials: S
authors.lastname: Yoshida
authors.forename: S
authors.initials: S
authors.lastname: Kishimoto
authors.forename: H
authors.initials: H
authors.lastname: Saito
mesh_terms: D000368:Aged; D000943:Antigens, Differentiation / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000276:immunology / Q000473:pathology / Q000532:radiotherapy*; D005260:Female; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007012:Hypopharyngeal Neoplasms / Q000276:immunology / Q000473:pathology / Q000532:radiotherapy*; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009959:Oropharyngeal Neoplasms / Q000276:immunology / Q000473:pathology / Q000532:radiotherapy*; D010610:Pharyngeal Neoplasms / Q000532:radiotherapy*; D011879:Radiotherapy Dosage
chemical_list: D000943:Antigens, Differentiation; D006684:HLA-DR Antigens