title: The influence of angiotensin II on catecholamine synthesis in neuronal cultures from rat brain.
abstract: Incubation of primary neuronal cultures prepared from the hypothalamus and brainstem of neonatal rats with angiotensin II (Ang-II) resulted in a concentration-dependent effect on the incorporation of [3H]-tyrosine ([3H]-Tyr) into [3H]-catecholamines ([3H]-CA). At concentrations of 1 nM-1 microM, Ang-II (60 min. incubation) caused significant decreases (31-52%) in neuronal [3H]-CA content compared with controls. Conversely, higher concentrations of Ang-II (10-100 microM; 60 min.) caused significant increases (20-60%) in neuronal [3H]-CA content compared with controls. Both of these effects were blocked by co-incubation with the Ang-II receptor antagonist Sar1Ile8-Ang-II. These observations demonstrate that neuronal cells in primary culture have the ability to synthesize [3H]-CA from [3H]-Tyr, and that Ang-II has a receptor-mediated biphasic influence on newly synthesized [3H]-CA (norepinephrine and dopamine).
authors.affiliation: Department of Physiology, College of Medicine, University of Florida, Gainesville 32610.
authors.forename: M R
authors.initials: MR
authors.lastname: MacLean
authors.forename: M K
authors.initials: MK
authors.lastname: Raizada
authors.forename: C
authors.initials: C
authors.lastname: Sumners
mesh_terms: D015059:1-Sarcosine-8-Isoleucine Angiotensin II / Q000494:pharmacology; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D001933:Brain Stem / Q000378:metabolism*; D002395:Catecholamines / Q000096:biosynthesis*; D002478:Cells, Cultured; D004298:Dopamine / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism*; D007700:Kinetics; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D009638:Norepinephrine / Q000096:biosynthesis; D051381:Rats; D011921:Rats, Inbred WKY; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D002395:Catecholamines; D000804:Angiotensin II; D014443:Tyrosine; D015059:1-Sarcosine-8-Isoleucine Angiotensin II; D004298:Dopamine; D009638:Norepinephrine