Inositol is a constituent of detergent-solubilized immunoaffinity-purified rat liver 5'-nucleotidase.
myo-Inositol analysis of detergent-solubilized immunoaffinity-purified rat liver 5'-nucleotidase showed the presence of 1 mol of myo-inositol/mol of enzyme monomer. This provides unequivocal evidence that the ectoenzyme 5'-nucleotidase is attached to liver membranes by a glycosyl-phosphatidylinositol lipid anchor.
Bailyes
E M
EM
Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital, U.K.
Ferguson
M A
MA
Colaco
C A
CA
Luzio
J P
JP
D015720:5'-Nucleotidase / Q000032:analysis* / Q000302:isolation & purification; D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry; D007294:Inositol / Q000032:analysis*; D008099:Liver / Q000201:enzymology*; D051381:Rats
D007294:Inositol; D015720:5'-Nucleotidase