Immunoelectron microscopic studies on the location of ribosomal proteins on the surface of the 40S ribosomal subunit from rat liver.
Seven ribosomal proteins have been localized by means of immunoelectron microscopy on the surface of the 40S ribosomal subunit from rat liver using monospecific antibodies. The location of ribosomal proteins S13/16, S19, and S24 is described for the first time, and that of ribosomal proteins S2, S3, S3a, and S7, which has been published previously on the basis of experiments performed with less well characterized antibody preparations [Lutsch et al., Mol. Gen. Genet. 176, 281-291 (1979) and Biomed. Biochim. Acta 42, 705-723 (1983)], is corrected in this paper. The results are discussed with respect to the involvement of these proteins in functional sites of the 40S ribosomal subunit.
Lutsch
G
G
Central Institute of Molecular Biology, Academy of Sciences of the GDR, Berlin-Buch.
Stahl
J
J
Kärgel
H J
HJ
Noll
F
F
Bielka
H
H
D000814:Aniline Compounds; D000818:Animals; D000906:Antibodies; D002499:Centrifugation, Density Gradient; D007150:Immunohistochemistry; D008099:Liver / Q000032:analysis / Q000648:ultrastructure*; D008854:Microscopy, Electron; D051381:Rats; D012269:Ribosomal Proteins / Q000032:analysis*; D012270:Ribosomes / Q000032:analysis* / Q000276:immunology / Q000648:ultrastructure
D000814:Aniline Compounds; D000906:Antibodies; D012269:Ribosomal Proteins; C512839:Rps13 protein, rat; C489055:Rps24 protein, rat; C023509:ribosomal protein S16; C040343:ribosomal protein S19; C030004:ribosomal protein S2; C044152:ribosomal protein S3; C037442:ribosomal protein S7; C487557:4-trifluoromethylaniline