Members of the RCK potassium channel family are differentially expressed in the rat nervous system.
mRNAs encoding four members of the RCK potassium channel family, named RCK1, RCK3, RCK4 and RCK5 have been analyzed by RNA blot hybridization experiments using specific RNA probes. Each probe recognizes a single mRNA species, their sizes ranging from approximately 4600 nucleotides up to approximately 11,000 nucleotides. The expression of RCK mRNAs as well as their developmental appearance in different regions of the central and peripheral rat nervous system has been investigated. The two most abundant RCK potassium channel mRNAs (RCK1 and RCK5) are predominantly expressed in the adult nervous system. RCK3 and RCK4 mRNAs are present throughout all developmental stages studied. The temporal and regional patterns observed are specific for each RCK potassium channel mRNA indicating that specific regulation of expression occurs. Differential mRNA expression might provide one mechanism for the generation of potassium channel diversity in vivo.
Beckh
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S
Lehrstuhl für Biochemie, Ruhr-Universität Bochum, FRG.
Pongs
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O
D000818:Animals; D001921:Brain / Q000378:metabolism*; D003391:Cranial Nerves / Q000378:metabolism*; D005727:Ganglia, Spinal / Q000378:metabolism; D005786:Gene Expression Regulation; D008565:Membrane Proteins / Q000235:genetics*; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D010525:Peripheral Nerves / Q000378:metabolism*; D010957:Plasmids; D015221:Potassium Channels / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D015183:Restriction Mapping; D013116:Spinal Cord / Q000378:metabolism; D014158:Transcription, Genetic
D008565:Membrane Proteins; D015221:Potassium Channels; D012333:RNA, Messenger