Absorption of triglycerides in the absence of lipase.
Medium chain triglycerides are considered to be readily absorbed intact in the absence of pancreatic lipase, unlike long chain triglycerides. Commercial medium chain triglyceride oils comprise various medium chain fatty acids from 6 to 12 carbons in length resulting in triglyceride molecules of different sizes and molecular weights. The effect of molecular weight and hence fatty acid chain length on the efficiency of intact medium chain triglyceride absorption is unknown. Therefore, this study measured, using a single-pass marker perfusion technique, intestinal jejunum absorption of five medium chain and one long chain triglycerides in anesthetized Sprague-Dawley rats. The molecular weights of the five medium chain triglycerides were 470.7, 498.8, 526.8, 554.9, 639.0, and the long chain triglyceride, 885.4. Residual luminal pancreatic lipase was removed prior to lipid perfusion. This study demonstrated that medium chain triglycerides were absorbed in the absence of lipase whereas long chain triglyceride was not. There was no significant variation in the absorption of the five different medium chain triglycerides perfused. The molecular weight of the medium chain triglyceride did not affect its intact absorption by the small intestine.
Chow
B P
BP
GI Research Group, Faculty of Medicine, University of Calgary, Alta, Canada.
Shaffer
E A
EA
Parsons
H G
HG
D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism; D002849:Chromatography, Gas; D007408:Intestinal Absorption; D008049:Lipase / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D010179:Pancreas / Q000201:enzymology; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000493:pharmacokinetics*; D014304:Triolein / Q000378:metabolism
D001647:Bile Acids and Salts; D014280:Triglycerides; D014304:Triolein; D008049:Lipase