Lymphoid and non-lymphoid cells in the adenoid of children with otitis media with effusion: a comparative study.
We characterized on immuno- and enzymecytochemical level the lymphoid and non-lymphoid cells in the adenoid of children with upper respiratory tract infections (URI) and otitis media with effusion (OME) and compared these with the adenoid of children with URI without OME and with the adenoid of 'healthy' children and adults. Besides macrophages and dendritic cells we also showed the presence of MHC class II positive, ciliated, epithelial cells. These non-lymphoid cells were present in all adenoids. However, their number was less than 1% of all cells. We found no difference in lymphocyte subsets from children with URI + OME compared with those from children with URI alone. These two groups showed a significant decrease of CD8-positive (suppressor/cytotoxic) cells and a slight increase in CD22-positive B cells in comparison to 'healthy' children. No difference was found in percentages of CD4-positive (helper/inducer) cells. The localization of the lymphoid subsets in adenoids of children with URI and/or OME did not differ from those of 'healthy' children and adults.
van Nieuwkerk
E B
EB
Department Otorhinolaryngology, University Hospital, Amsterdam, The Netherlands.
de Wolf
C J
CJ
Kamperdijk
E W
EW
van der Baan
S
S
D000234:Adenoids / Q000166:cytology* / Q000276:immunology; D002452:Cell Count; D002648:Child; D003713:Dendritic Cells; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D008264:Macrophages; D010034:Otitis Media with Effusion / Q000276:immunology / Q000473:pathology*