title: CD4-like molecules in human sperm.
abstract: The expression of a molecule recognized by CD4 monoclonal antibodies was investigated on human sperm using immunolabelling, biochemical and immunochemical methods. Flow cytometry detected a significant fluorescence signal. SDS-PAGE analysis and Western blotting identified a molecule of 60 kDa, consistent with a CD4-like structure as confirmed after selective immunoseparation. Additional bands reacting with anti-CD4 were found in sperm extracts (73 kDa) and seminal fluid (90 kDa). These data indicate that sperm express a molecule similar to the receptor for HIV described on mononuclear cells.
authors.affiliation: Laboratoire d'Immunologie, Faculté de Médecine, Université de Nancy, Vandoeuvre-les Nancy, France.
authors.forename: B
authors.initials: B
authors.lastname: Gobert
authors.forename: C
authors.initials: C
authors.lastname: Amiel
authors.forename: J Q
authors.initials: JQ
authors.lastname: Tang
authors.forename: P
authors.initials: P
authors.lastname: Barbarino
authors.forename: M C
authors.initials: MC
authors.lastname: Béné
authors.forename: G
authors.initials: G
authors.lastname: Faure
mesh_terms: D015703:Antigens, CD / Q000032:analysis*; D015153:Blotting, Western; D015704:CD4 Antigens / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D006801:Humans; D007526:Isoelectric Point; D008297:Male; D008970:Molecular Weight; D013094:Spermatozoa / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D015704:CD4 Antigens