Rapid detection of influenza virus H1 by the polymerase chain reaction.
We applied a combination of reverse transcription (RT) with the polymerase chain reaction (PCR) for a rapid detection of influenza virus H1 subtype. We amplified a 441 bp segment of relatively high genetic stability of the hemagglutinin gene. Experimental conditions were established using plasmid DNA and infected cell cultures. The test was applied to 28 nasopharyngeal lavages from patients, two of which were positive for influenza virus H1. When the amplified DNA of a positive sample was sequenced we found 97% homology with the recent strain A/USSR/70.
Bressoud
A
A
Department of Carcinogenesis, Swiss Institute for Experimental Cancer Research, Epalinges, Lausanne.
Whitcomb
J
J
Pourzand
C
C
Haller
O
O
Cerutti
P
P
D001482:Base Composition; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral / Q000235:genetics*; D009980:Influenza A virus / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification
D004279:DNA, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral; D015345:Oligonucleotide Probes; D012367:RNA, Viral