title: Phenylalanine hydroxylation in isolated rat kidney tubules.
abstract: 1. Phenylalanine hydroxylation has been demonstrated to occur in isolated rat kidney tubules under physiological conditions. 2. The hydroxylation flux response is hyperbolic with apparent Km and Vmax values of ca 85 microM phenylalanine and 49 nmol tyrosine formed/mg dry wt per hr respectively. 3. Hydroxylation in kidney tubules is substantially less sensitive to effectors of cyclic AMP turnover and Ca2+ mobilization than phenylalanine hydroxylation in isolated liver cells.
authors.affiliation: Department of Biochemistry, University of Liverpool, England.
authors.forename: D
authors.initials: D
authors.lastname: Askin
authors.forename: A K
authors.initials: AK
authors.lastname: Green
authors.forename: A J
authors.initials: AJ
authors.lastname: Dickson
authors.forename: M J
authors.initials: MJ
authors.lastname: Fisher
mesh_terms: D000818:Animals; D006706:Homeostasis; D006900:Hydroxylation; D066298:In Vitro Techniques; D007684:Kidney Tubules / Q000201:enzymology / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D010649:Phenylalanine / Q000378:metabolism*; D010651:Phenylalanine Hydroxylase / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D010649:Phenylalanine; D010651:Phenylalanine Hydroxylase