Comparison of the CopB systems of plasmids R1 and ColV2-K94: a single base alteration in CopB gene is responsible for the increased copy number of the low copy number plasmid ColV2-K94.
We have isolated a deletion mutation and a point mutation in the copB gene of the replication region Repl of the IncFI plasmid ColV2-K94. Subsequently, this copB gene with and without point mutation was cloned and sequenced, and the point mutation was mapped in the coding region of copB with a change of one amino acid from arginine to serine. Furthermore, this copB mutant had an approximately 10-fold increase in copy number. The CopB-phenotype of ColV2-K94 could be complemented in trans by the copB gene of coresident IncFII plasmids such as R1 and R538, but not R100, suggesting that ColV2-K94 and R1 or R538 contain the same copB allele.
Banerjee
A
A
Department of Immunology and Microbiology, WSU School of Medicine, Detroit, MI 48201.
Weber
P C
PC
Palchaudhuri
S
S
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D003086:Bacteriocin Plasmids; D001483:Base Sequence; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D010957:Plasmids; D011815:R Factors; D012093:Replicon; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D001426:Bacterial Proteins; D004269:DNA, Bacterial