Continuous in vivo measurement of hepatic lipoperoxidation using chemiluminescence: halothane and chloroform compared.
Hepatic necrosis, rarely occurring after administration of modern halogenated general anesthetics, has been ascribed to free radical-induced lipoperoxidation. As evaluation of hepatic chemiluminescence has been shown to provide a noninvasive assay for oxidative radical reactions, we have made continuous in vivo measurements of hepatic light output in the phenobarbital-induced halothane anesthetized rat and compared them with observations made during chloroform anesthesia. Chloroform produced a far more rapid and more profound increase in chemiluminescence, a phenomenon probably related to depletion of hepatic glutathione. These findings are consistent with significant differences in hepatic pathology associated with the two agents.
Cohen
P J
PJ
Department of Anesthesia, University of Pennsylvania, Philadelphia 19104-4282.
Chance
B
B
D000818:Animals; D002725:Chloroform / Q000633:toxicity*; D006221:Halothane / Q000633:toxicity*; D008099:Liver / Q000187:drug effects*; D008163:Luminescent Measurements; D008297:Male; D008991:Monitoring, Physiologic / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains
D002725:Chloroform; D006221:Halothane