Induction of an acute erosive monarticular arthritis in mice by interleukin-1 and methylated bovine serum albumin.
We examined the effect of interleukin-1 (IL-1) administration on a mild and transient inflammatory response in the knees of mice injected intraarticularly with methylated bovine serum albumin (mBSA). Injection of mBSA on day 0 into nonsensitized mice caused a weak inflammatory response confined to the infrapatellar fat pads and involved infiltration by mononuclear cells, neutrophils, and eosinophils. The response developed between days 4 and 7 and resolved by day 28. No erosion of cartilage or subchondral bone was seen. In contrast, mBSA-treated mice injected with recombinant human IL-1 beta subcutaneously in the ipsilateral footpad on days 0-3 developed a severe monarticular arthritis in the antigen-injected knee. Pannus developed, extending over the articular surfaces, and extensive erosion of cartilage and subchondral bone occurred. Multinucleated giant cells, together with fibrin-like material, were observed at sites of active bone erosion and debris, and large numbers of neutrophils were seen in the joint space. These pathologic features represent a new arthritis model in which IL-1 profoundly augments a weak inflammatory response and induces acute erosive joint destruction, supporting the hypothesis that IL-1 is an important cytokine in the pathogenesis of arthritis.
Staite
N D
ND
Upjohn Company, Kalamazoo, MI 49001.
Richard
K A
KA
Aspar
D G
DG
Franz
K A
KA
Galinet
L A
LA
Dunn
C J
CJ
D000208:Acute Disease; D000818:Animals; D001168:Arthritis / Q000209:etiology*; D001169:Arthritis, Experimental / Q000209:etiology* / Q000473:pathology; D002358:Cartilage, Articular / Q000473:pathology; D005260:Female; D007270:Injections, Intra-Articular; D007375:Interleukin-1 / Q000008:administration & dosage; D007719:Knee Joint / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils; D012710:Serum Albumin, Bovine / Q000008:administration & dosage; D013997:Time Factors
D007375:Interleukin-1; C029180:methylated bovine serum albumin; D012710:Serum Albumin, Bovine