14, 15-Epoxyeicosatrienoic acid promotes endothelial cell dependent adhesion of human monocytic tumor U937 cells.
Arachidonic acid (AA) can be metabolized in endothelial cells (EC) to a series of epoxides via cytochrome P-450 epoxygenase with 14,15 epoxyeicosatrienoic acid (14,15-EET) as the major product. In this communication we report that 14,15-EET significantly enhances U937 cell attachment to EC with maximal cell attachment at 2.5 to 5 x 10(-7) M 14,15-EET. Thus, 14,15-EET may play a substantial role in inflammation and/or atherogenesis by inducing monocyte attachment to EC.
Pritchard
K A
KA
Department of Physiology, New York Medical College, Valhalla 10595.
Tota
R R
RR
Stemerman
M B
MB
Wong
P Y
PY
D015126:8,11,14-Eicosatrienoic Acid / Q000031:analogs & derivatives / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D004727:Endothelium / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005456:Fluorescent Dyes; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D011453:Prostaglandins / Q000032:analysis; D013917:Thrombin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D005231:Fatty Acids, Unsaturated; D005456:Fluorescent Dyes; D011453:Prostaglandins; C046782:14,15-epoxy-5,8,11-eicosatrienoic acid; D007770:L-Lactate Dehydrogenase; D013917:Thrombin; D015126:8,11,14-Eicosatrienoic Acid