Neural systems contributing to acetylcholinesterase histochemical staining in primary visual cortex of the adult rat.
Histochemical studies demonstrate that cortical area 17 (primary visual cortex) of the adult rat displays a characteristic laminar pattern of acetylcholinesterase (AChE) activity. While AChE-positive axons are found throughout the cortical layers, most intense staining occurs in a band that corresponds to layer V and the deep portion of layer IV. The present studies were directed toward determining the neural systems containing this AChE activity. Unilateral electrolytic or excitatory amino acid induced lesions of the basal forebrain result in reductions of AChE staining in ipsilateral visual cortex, particularly in layers IV and V. Electrolytic or scalpel lesions, placed in white matter underlying dorsal and lateral neocortex to interrupt basal forebrain projections to visual cortex, also reduce AChE staining in visual cortex. Lesions in the cingulate bundle and supracallosal stria reduced AChE staining retrosplenial cortex but did not affect staining visual cortex. Placement of electrolytic lesions in the hypothalamus produced no detectable change in the pattern of AChE in visual cortex. Electrolytic lesions in the midbrain tegmentum, placed to interrupt ascending axons from brainstem monoamine neurons, produced no detectable change in the pattern of AChE in visual cortex. Placement of lesions in the dorsal thalamus that include all of the dorsal lateral geniculate nucleus did not alter AChE staining in visual cortex. The results indicate that AChE activity in adult visual cortex is found primarily within afferent axons from the basal forebrain system. These data demonstrate further that the AChE staining characteristic of adult visual cortex is associated with neural systems that are distinctly different from those associated with AChE staining in visual cortex of the infant rat.
Robertson
R T
RT
Department of Anatomy and Neurobiology, College of Medicine, University of California, Irvine 92717.
Fehrenbach
C J
CJ
Yu
J
J
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001933:Brain Stem / Q000166:cytology; D005260:Female; D005625:Frontal Lobe / Q000166:cytology; D005829:Geniculate Bodies / Q000166:cytology; D006651:Histocytochemistry; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014793:Visual Cortex / Q000166:cytology / Q000201:enzymology*; D014795:Visual Pathways / Q000033:anatomy & histology*
D000110:Acetylcholinesterase