Induction of interleukin-1 alpha and beta gene transcription in mouse peritoneal exudate cells after intraperitoneal infection with herpes simplex virus-1.
Macrophages have been shown to play a determining role in the immune defense against herpes simplex virus-1 (HIV-1) intraperitoneal infection in the mouse. In the present study, the effect of HSV-1 infection on interleukin-1 alpha and beta gene transcription in peritoneal exudate cells was investigated. HSV-1 infection was found to induce interleukin-1 alpha and beta gene transcription in these cells. Induction of the interleukin-1 beta gene initiated 6 h postinfection (p.i.) and terminated at 48 h p.i. In contrast, interleukin-1 alpha RNA was detectable at high levels at 6h p.i. but not at 24 h p.i. Inactivation of virus prior to infection prevented HSV-1-induced IL-1 gene induction, indicating that only infectious virus is able to stimulate IL-1 gene transcription. These findings are discussed in relation to the role of macrophages in the immunological mechanisms of defense against HSV-1 infection.
Sprecher
E
E
Department of Molecular Virology, Faculty of Medicine, Hebrew University, Jerusalem, Israel.
Becker
Y
Y
D000818:Animals; D015152:Blotting, Northern; D015342:DNA Probes; D003720:Densitometry; D005260:Female; D015870:Gene Expression; D006561:Herpes Simplex / Q000276:immunology*; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D010538:Peritonitis / Q000209:etiology / Q000276:immunology*; D012367:RNA, Viral / Q000096:biosynthesis; D014158:Transcription, Genetic
D015342:DNA Probes; D007375:Interleukin-1; D012367:RNA, Viral