title: Analysis of factors influencing clonogenic growth in vitro of cells from ovarian carcinoma patients.
abstract: Clonogenic growth (defined as the formation of greater than or equal to 5 colonies per 5 x 10(5) viable nucleated cells per plate) of ovarian cancer specimens assessed in our clonogenic assay system was significantly associated with the proportion of tumor cells in the suspensions plated (N = 87; P = 0.0006), although there was no quantitative relationship with the corresponding plating efficiencies. An inverse correlation was observed between monocytes/macrophages/mesothelial cells (M) proportion and clonogenic growth (P = 0.013). These associations were most evident when only effusions were considered. Univariate analyses identified tumor cell content, M proportion and, to a lesser degree, granulocyte content as the only factors out of 12 examined to be correlated with colony formation. Multivariate analysis using a logistic regression model identified the proportion of tumor cells as the only significant factor predicting clonogenic growth in vitro (P = 0.0006). The overall accuracy of prediction for growth or non-growth was 63.2%.
authors.affiliation: Department of Chemotherapy, University of Vienna, Austria.
authors.forename: C
authors.initials: C
authors.lastname: Dittrich
authors.forename: E
authors.initials: E
authors.lastname: Dittrich
authors.forename: F
authors.initials: F
authors.lastname: Wrba
authors.forename: M
authors.initials: M
authors.lastname: Hudec
authors.forename: T
authors.initials: T
authors.lastname: Grunt
authors.forename: H
authors.initials: H
authors.lastname: Salzer
authors.forename: P
authors.initials: P
authors.lastname: Sevelda
authors.forename: J
authors.initials: J
authors.lastname: Eliason
mesh_terms: D000362:Agar; D000704:Analysis of Variance; D002452:Cell Count; D002455:Cell Division; D002498:Centrifugation; D005260:Female; D006098:Granulocytes; D006801:Humans; D066298:In Vitro Techniques; D014411:Neoplastic Stem Cells / Q000166:cytology*; D010051:Ovarian Neoplasms / Q000473:pathology*; D010586:Phagocytes; D011336:Probability; D012044:Regression Analysis; D014410:Tumor Stem Cell Assay
chemical_list: D000362:Agar