title: Phylogenetic relationships of Cryptosporidium determined by ribosomal RNA sequence comparison.
abstract: Reverse transcription of total cellular RNA was used to obtain a partial sequence of the small subunit ribosomal RNA of Cryptosporidium, a protist currently placed in the phylum Apicomplexa. The semi-conserved regions were aligned with homologous sequences in a range of other eukaryotes, and the evolutionary relationships of Cryptosporidium were determined by two different methods of phylogenetic analysis. The prokaryotes Escherichia coli and Halobacterium cuti were included as outgroups. The results do not show an especially close relationship of Cryptosporidium to other members of the phylum Apicomplexa.
authors.affiliation: Department of Clinical Microbiology, Flinders University School of Medicine, Flinders Medical Centre, Bedford Park, South Australia.
authors.forename: A M
authors.initials: AM
authors.lastname: Johnson
authors.forename: R
authors.initials: R
authors.lastname: Fielke
authors.forename: R
authors.initials: R
authors.lastname: Lumb
authors.forename: P R
authors.initials: PR
authors.lastname: Baverstock
mesh_terms: D000818:Animals; D001483:Base Sequence; D003044:Coccidia / Q000145:classification*; D003458:Cryptosporidium / Q000145:classification* / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics*
chemical_list: D012335:RNA, Ribosomal