Coding sequence and flanking regions of the mouse vimentin gene.
Using a polyclonal antibody, a cDNA clone coding for part of mouse vimentin was identified in a lambda gt11 expression library. DNA from this clone was used to screen a genomic library from Ehrlich Ascites Tumor cells for the mouse vimentin gene. A clone was found which contained the whole coding sequence and a large part of the 5'- and 3'-untranslated sequences. It was used to prepare a construct equivalent to a full-length cDNA clone. Extensive homologies to the vimentin sequence from other species were found for the coding and 3'-untranslated sequences and the promoter region.
Hennekes
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H
Max-Planck-Institut für Zellbiologie, Rosenhof, Ladenburg bei Heidelberg, Federal Republic of Germany.
Kühn
S
S
Traub
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P
D000818:Animals; D001483:Base Sequence; D005091:Exons; D005796:Genes; D006801:Humans; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured; D014746:Vimentin / Q000235:genetics*
D014746:Vimentin