Relationship between vessel wall 13-HODE synthesis and vessel wall thrombogenicity following injury: influence of salicylate and dipyridamole treatment.
We performed studies to determine the relationship between injured vessel wall thrombogenicity, vessel wall 13-hydroxyoctadecadienoic acid (13-HODE) synthesis and cAMP levels in rabbit treated with salicylate or dipyridamole. Injured vessel wall thrombogenicity was measured as the number of 3H-adenine labelled platelets adhered to the subendothelial basement membrane exposed by air injury in carotid arteries of rabbits treated orally with salicylate or dipyridamole. Vessel wall 13-HODE was measured by HPLC and vessel wall cAMP was measured by RIA. Vessel wall thrombogenicity was increased two-fold in rabbits treated with salicylate and decreased by half in rabbits treated with dipyridamole. The levels of vessel wall cAMP levels were correlated both with the plasma dipyridamole levels and increases in 13-HODE synthesis. cAMP levels were unaffected by salicylate treatment, but 13-HODE synthesis was decreased. We conclude that there is a significant relationship between vessel wall cAMP levels and 13-HODE synthesis, which in turn, influences subsequent vessel wall thrombogenicity.
Weber
E
E
Department of Pathology, McMaster University, Hamilton, Canada.
Haas
T A
TA
Muller
T H
TH
Eisert
W G
WG
Hirsh
J
J
Richardson
M
M
Buchanan
M R
MR
D000818:Animals; D002339:Carotid Arteries; D004176:Dipyridamole / Q000494:pharmacology / Q000627:therapeutic use*; D004730:Endothelium, Vascular / Q000293:injuries* / Q000378:metabolism; D008041:Linoleic Acids / Q000096:biosynthesis* / Q000502:physiology; D010973:Platelet Adhesiveness / Q000187:drug effects*; D011817:Rabbits; D012980:Sodium Salicylate / Q000494:pharmacology / Q000627:therapeutic use*; D013927:Thrombosis / Q000378:metabolism / Q000517:prevention & control*
D008041:Linoleic Acids; C024348:13-hydroxy-9,11-octadecadienoic acid; D004176:Dipyridamole; D012980:Sodium Salicylate