Variation of the surface properties of Bacillus licheniformis according to age, temperature and aeration.
The surface properties of Bacillus licheniformis DSM 603 have been examined as a function of age during cultures in baffled and non-baffled Erlenmeyer flasks, at 30 and 37 degrees C. The elemental composition of the surface was determined by X-ray photoelectron spectroscopy and its variation was compared with the electrophoretic mobility and water contact angle. The variations of the surface phosphate concentration during the cultures and according to growth conditions indicate that the determining factor is the total cell concentration. The P/C atomic concentration ratio remains in a range where its variation does not influence the electrophoretic mobility. The decrease and increase of hydrophobicity during cultures at 30 degrees C in baffled and normal flasks, respectively, are paralleled by a variation in the O/C ratio.
Herben
P F
PF
Université Catholique de Louvain, Unité de Chimie des Interfaces, Louvain-la-Neuve, Belgium.
Mozes
N
N
Rouxhet
P G
PG
D001407:Bacillus / Q000254:growth & development; D010100:Oxygen / Q000378:metabolism*; D013057:Spectrum Analysis; D013696:Temperature
D010100:Oxygen