A new human plasma cell line, Karpas 620, with translocations involving chromosomes 1, 11 and 14.
We report here the establishment of a new cell line, Karpas 620 (K620), from the peripheral blood of an elderly woman with an IgG-kappa plasma cell leukaemia (PCL). The line has the same hypotetraploid karyotype as the fresh cells from the patient. The cultured cells have the ultrastructural appearance of plasma cells with abundant rough endoplasmic reticulum (RER) and secrete kappa light chain. They are positive for surface antigens HLA DR, and WR17 (CD 37) and negative for CD1, CD3, CD4 and CD8. Using high resolution (HR) cytogenetic analysis it has been possible to identify all the marker chromosomes including several rearrangements commonly seen in malignancies of B cell lineage. These are a 14q+ marker with a typical 'Burkitt' morphology der(14)(pter----q32.3::8q24.1----qter) but with no reciprocal 8q-, and three translocations involving chromosome 11 at q13 with partners other than chromosome 14, namely 1q32.1, 8q24.22 and 13q14.3. An earlier report of molecular studies on the DNA of K620 has shown a rearrangement near the region on 11q13 designated BCL-1 (Rabbitts et al. 1988). This is the first report of a rearrangement in the region of 11q13 in a cell line originating from a case of plasma cell leukaemia.
Nacheva
E
E
Department of Haematology, University of Cambridge Clinical School.
Fischer
P E
PE
Sherrington
P D
PD
Labastide
W
W
Lawlor
E
E
Conneally
E
E
Blaney
C
C
Hayhoe
F G
FG
Karpas
A
A
D000368:Aged; D000951:Antigens, Neoplasm / Q000032:analysis; D002460:Cell Line / Q000502:physiology*; D002878:Chromosomes, Human, Pair 1; D002880:Chromosomes, Human, Pair 11; D002883:Chromosomes, Human, Pair 14; D005260:Female; D006801:Humans; D007621:Karyotyping; D007952:Leukemia, Plasma Cell / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D014178:Translocation, Genetic / Q000235:genetics*; D014407:Tumor Cells, Cultured / Q000502:physiology
D000951:Antigens, Neoplasm