Modulation of ATP-sensitive potassium channel activity by flash-photolysis of 'caged-ATP' in rat heart cells.
We have used 'caged-ATP' to investigate the kinetic behavior of KATP channels in ventricular cells from rat heart. In whole cells, loaded with 'caged-ATP', an increase of intracellular [ATP] following a UV light flash produced a decrease of KATP channel current that was too slow (tau approximately 300 ms) to be explained by the expected time-course of ATP release (tau approximately 3 ms) and the time-course of channel blockade by ATP (tau approximately 20 ms). In isolated membrane patches, caged-ATP itself caused partial blockade of KATP) channels. Under these conditions, photorelease of ATP caused channel activity to decline further. The results suggest that caged-ATP can bind to the KATP) channel but, on binding, decreases the open probability to a lesser extent than does ATP. Additionally, the observations indicate that for photolytically-generated ATP to bind to the channel, caged-ATP must first unbind (slowly) from the channel. We conclude that 'caged-ATP' is not fully caged with respect to its allosteric action on the KATP channel.
Nichols
C G
CG
Department of Physiology, University of Maryland, Baltimore 21201.
Niggli
E
E
Lederer
W J
WJ
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000502:physiology*; D000818:Animals; D006321:Heart / Q000502:physiology*; D006352:Heart Ventricles; D066298:In Vitro Techniques; D009206:Myocardium / Q000166:cytology*; D010782:Photolysis; D015221:Potassium Channels / Q000502:physiology*; D051381:Rats
D015221:Potassium Channels; C029273:P(3)-1-(2-nitro)phenylethyladenosine 5'-triphosphate; D000255:Adenosine Triphosphate