title: [Differentiation of human sperm cells using flow cytometry].
abstract: An improved method for differential staining and high-resolution flow cytometric measurement of human sperm cells is presented. Involving a mild pretreatment with citric acid/detergent and staining with DAPI, the new procedure provides excellent preservation and good discrimination of all cells present in normal and pathologic semen samples.
authors.affiliation: Abteilung Experimentelle Tumorforschung, Fachklinik Hornheide, Münster.
authors.forename: F J
authors.initials: FJ
authors.lastname: Otto
authors.forename: H
authors.initials: H
authors.lastname: Hettwer
authors.forename: N
authors.initials: N
authors.lastname: Hofmann
mesh_terms: D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000473:pathology*; D013076:Sperm Count; D013081:Sperm Motility / Q000502:physiology; D013091:Spermatogenesis / Q000502:physiology; D013094:Spermatozoa / Q000473:pathology*
vernacular_title: Differenzierung menschlicher Spermazellen mit Hilfe der Durchflusszytometrie.