Radioprotection of mice with interleukin-1: relationship to the number of erythroid and granulocyte-macrophage colony-forming cells.
This report presents the results of an investigation of changes in the number of erythroid and granulocyte-macrophage colony-forming cells (GM-CFC) that had occurred in tissues of normal B6D2F1 mice 20 h after administration of a radioprotective dose (150 ng) of human recombinant interleukin-1 (rIL-1). Neutrophilia in the peripheral blood and changes in the tissue distribution of GM-CFC demonstrated that cells were mobilized from the bone marrow in response to rIL-1 injection. For example, 20 h after rIL-1 injection marrow GM-CFC numbers were 80% of the numbers in bone marrow from saline-injected mice. Associated with this decrease there was a twofold increase in the number of peripheral blood and splenic GM-CFC. Also, as determined by hydroxyurea injection, there was an increase in the number of GM-CFC in S phase of the cell cycle in the spleen, but not in the bone marrow. Data in this report suggest that when compared to the spleen, stimulation of granulopoiesis after rIL-1 injection is delayed in the bone marrow. Also, the earlier recovery of GM-CFC in the bone marrow of irradiated mice is not dependent upon an increase in the number of GM-CFC at the time of irradiation.
Schwartz
G N
GN
Transplantation Laboratory, American Red Cross, Rockville, Maryland 20855.
Patchen
M L
ML
Neta
R
R
MacVittie
T J
TJ
D000818:Animals; D003114:Colony-Forming Units Assay; D015672:Erythroid Precursor Cells / Q000166:cytology*; D005260:Female; D006098:Granulocytes / Q000166:cytology*; D007375:Interleukin-1 / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D011994:Recombinant Proteins
D007375:Interleukin-1; D011837:Radiation-Protective Agents; D011994:Recombinant Proteins