Pairing for recombination in LGV of Caenorhabditis elegans: a model based on recombination in deficiency heterozygotes.
The effect of deficiencies on recombination was studied in Caenorhabditis elegans. Heterozygous deficiencies in the left half of linkage group V [LGV(left)] were shown to inhibit recombination to their right. Fourteen deficiencies, all to the left of unc-46, were analyzed for their effect on recombination along LGV. The deficiencies fell into two groups: 10 "major inhibitors" which reduce recombination to less than 11% of the expected rate between themselves and unc-46; and four "minor inhibitors" which reduce recombination, but to a much lesser extent. All four minor inhibitors delete the left-most known gene on the chromosome, while six of the ten major inhibitors do not (i.e., these are "internal" deficiencies). Where recombination could be measured on both sides of a deficiency, recombination was inhibited to the right but not to the left. In order to explain these results we have erected a model for the manner in which pairing for recombination takes place. In doing so, we identify a new region of LGV, near the left terminus, that is important for the pairing process.
Rosenbluth
R E
RE
Department of Biological Sciences, Simon Fraser University, Burnaby, British Columbia, Canada.
Johnsen
R C
RC
Baillie
D L
DL
D000818:Animals; D002107:Caenorhabditis / Q000235:genetics*; D008040:Genetic Linkage; D006579:Heterozygote; D008957:Models, Genetic; D009154:Mutation; D011995:Recombination, Genetic