Motor response of the human isolated small intestine and urinary bladder to porcine neuromedin U-8.
1. Porcine neuromedin U-8 produced a concentration (0.3 nM-1 microM)-dependent contraction of the longitudinal muscle of the human isolated ileum, which was unaffected by either atropine (1 microM) or tetrodotoxin (1 microM). 2. By contrast, neuromedin U-8 had only a weak effect on the circular muscle of the human isolated ileum. 3. Neuromedin U-8 also produced a concentration-dependent contraction of mucosa-free muscle strips from the dome of the human isolated urinary bladder, its action being unaffected by either atropine or tetrodotoxin. 4. These findings indicate that neuromedin U-8 exerts a direct contractile effect on smooth muscle of the human intestinal and urinary tract.
Maggi
C A
CA
Pharmacology Department, A. Menarini Pharmaceuticals, Florence, Italy.
Patacchini
R
R
Giuliani
S
S
Turini
D
D
Barbanti
G
G
Rovero
P
P
Meli
A
A
D000368:Aged; D000818:Animals; D004558:Electric Stimulation; D006801:Humans; D066298:In Vitro Techniques; D007421:Intestine, Small / Q000187:drug effects; D008875:Middle Aged; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects*; D009479:Neuropeptides / Q000494:pharmacology*; D013552:Swine; D001743:Urinary Bladder / Q000187:drug effects
D009479:Neuropeptides; C046270:neuromedin U 8