Unexpected elution behaviour of peptides with various reversed-phase columns.
Gastrin and cholecystokinin peptides were separated by reversed-phase chromatography on conventional bristle-type and polymer-coated stationary phases. The retention of the sulphated and non-sulphated isomeric forms of both peptides is governed by the structure of the peptide, the net charge and additional polar interactions with the stationary phases. Polymer-coated phases are optimum for separations according to chain length, whereas polar interactions are required for the separation of sulphated and non-sulphated peptides of identical chain length.
Engelhardt
H
H
Angewandte Physikalische Chemie, Universität des Saarlandes, Saarbrücken, F.R.G.
Appelt
G
G
Schweinheim
E
E
D002766:Cholecystokinin / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005755:Gastrins / Q000032:analysis; D010455:Peptides / Q000032:analysis*
D005755:Gastrins; D010455:Peptides; D002766:Cholecystokinin