title: The pharmacokinetics of chloramphenicol in plasma and saliva of dairy cows.
abstract: In the present study the pharmacokinetics of chloramphenicol were investigated in bovine plasma and saliva. The method of detection of chloramphenicol, a radio-immunoassay, requires only small sample volumes, no further clean-up, and a large number of samples can be processed simultaneously. This enabled the collection of a larger number of samples over a longer period of time than in previous studies. The intention was to obtain a complete pharmacokinetic profile of chloramphenicol, to evaluate it according to common open-compartment models, and to compare it with the results of previous studies. The radio-immunoassay results were confirmed by analysing a number of samples distributed over the concentration range of interest by a gas-chromatographic method with electron-capture detection. The suitability of saliva was evaluated as a possible means of monitoring compliance with the ban on chloramphenicol in the Federal Republic of Germany in milk-producing animals.
authors.affiliation: Federal Health Office, Institute of Veterinary Medicine, Berlin, FRG.
authors.forename: A
authors.initials: A
authors.lastname: Dotter
authors.forename: R
authors.initials: R
authors.lastname: Kroker
authors.forename: D
authors.initials: D
authors.lastname: Arnold
mesh_terms: D000818:Animals; D002417:Cattle / Q000097:blood / Q000378:metabolism*; D002701:Chloramphenicol / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D002849:Chromatography, Gas; D005260:Female; D007279:Injections, Subcutaneous / Q000662:veterinary; D011247:Pregnancy; D011863:Radioimmunoassay; D012463:Saliva / Q000378:metabolism*; D013997:Time Factors
chemical_list: D002701:Chloramphenicol