title: The fate of 131I-17-iodoheptadecanoic acid during lactate loading: its oxidation is strongly inhibited in favor of its esterification. A radiochemical study in the canine heart.
abstract: The influence of lactate loading on fatty acid metabolism (pH = 7.4) by the normal canine heart was investigated radiochemically using the radioiodinated fatty acid 131I-17-iodoheptadecanoic acid (131I-17-HDA). Fatty acid metabolism was studied during control conditions (n = 8) and after lactate loading (n = 7). In the canine heart total myocardial 131I-17-HDA radioactivity (uptake) was not changed during the lactate intervention. The oxidation decreased fivefold (measured as free 131I-iodide ion) from 70% to 14% (p less than 0.0001, Student's t-test). Thin-layer chromatography of cardiac lipids demonstrated that the non-oxidized 131I-17-HDA was mainly stored in the triglycerides and phosphoglycerides. These results suggest that lactate inhibits cardiac 131I-HDA oxidation.
authors.affiliation: Department of Cardiology, Free University Hospital, Amsterdam, The Netherlands.
authors.forename: C M
authors.initials: CM
authors.lastname: Duwel
authors.forename: F C
authors.initials: FC
authors.lastname: Visser
authors.forename: M J
authors.initials: MJ
authors.lastname: van Eenige
authors.forename: W
authors.initials: W
authors.lastname: Den Hollander
authors.forename: J P
authors.initials: JP
authors.lastname: Roos
mesh_terms: D000818:Animals; D004285:Dogs; D004951:Esterification; D005227:Fatty Acids / Q000378:metabolism*; D007457:Iodine Radioisotopes; D007773:Lactates / Q000494:pharmacology*; D009206:Myocardium / Q000378:metabolism*; D010084:Oxidation-Reduction
chemical_list: D005227:Fatty Acids; D007457:Iodine Radioisotopes; D007773:Lactates; C027252:17-iodoheptadecanoic acid