title: Murine monoclonal antibody with anti-e-like specificity: suitability for screening for e-negative cells.
abstract: A directly agglutinating murine monoclonal antibody of the IgG3 isotype has been produced after mice were immunized with papain-treated Bombay phenotype, e-positive red cells (RBCs). The antibody strongly agglutinated all e-positive RBCs and gave negative reactions with RBCs from Rhnull persons and those homozygous for Rh deletion genes. Variable reactivity was found with e-negative RBCs, ranging from weak to negative with R2R2, slightly stronger with r"r", and stronger still with RzRz. These reactions, together with results from tests with a large panel of Rh-variant RBCs, suggested a specificity for an epitope that is part of the e mosaic but is also expressed at least partially on e-negative RBCs and is influenced by the presence of C. This antibody has been standardized for use as a screening reagent for R2R2 cells by a microplate technique, and over 6000 donations have been screened in this way. No discrepancies have been found in confirmatory tests for e-negative status using human anti-e.
authors.affiliation: Glasgow and West of Scotland Regional Blood Transfusion Centre, Law Hospital, Carluke.
authors.forename: R H
authors.initials: RH
authors.lastname: Fraser
authors.forename: G
authors.initials: G
authors.lastname: Inglis
authors.forename: J C
authors.initials: JC
authors.lastname: Allan
authors.forename: M T
authors.initials: MT
authors.lastname: Murphy
authors.forename: E K
authors.initials: EK
authors.lastname: Allan
authors.forename: A
authors.initials: A
authors.lastname: Mackie
authors.forename: R
authors.initials: R
authors.lastname: Mitchell
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal; D000917:Antibody Formation; D000918:Antibody Specificity; D001788:Blood Grouping and Crossmatching; D007073:Immunoglobulin E / Q000276:immunology*; D051379:Mice
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; C026408:anti-IgE antibodies; D007073:Immunoglobulin E