title: Langerhans cells and mycosis fungoides--a critical overview of their pathogenic role in the disease.
abstract: Skin biopsies from seven patients with mycosis fungoides in various clinical stages (patches, plaques, nodules) were studied immunohistochemically and ultrastructurally, with the aim of investigating and quantifying the distribution of Langerhans cells and their relationships to mycosis cells. Our findings have revealed that in patches and plaques both Langerhans cells and mycosis cells were numerous in the epidermis. Notwithstanding this, in all the specimens examined, only one Langerhans cell forming close contact with a mycosis cell was detected. In the nodules, Langerhans cells and mycosis cells were sparse in the epidermis and no contacts were seen between them. Moreover, in all the patients studied, only a single Langerhans cell was found in the dermal infiltrate without any closely related mycosis cells. Conversely, numerous interdigitating cells have been found in the dermis of patches and plaques, often tightly adhering to mycosis cells. In the nodule, a few scattered interdigitating cells were seen, but often these had close contacts with neoplastic lymphoid cells. These findings indicate that close apposition between Langerhans cells and mycosis cells, which led previous authors to hypothesize a persistent stimulatory action of Langerhans cells on T lymphocytes, eventually leading to the malignant transformation of the latter, is unusual in mycosis fungoides. Therefore, if such a pathogenic role may be attributed to accessory cells in mycosis fungoides it is more probably exerted by dermal interdigitating cells and not by Langerhans cells, as previously proposed.
authors.affiliation: Department of Human Anatomy and Histology, V. le G.Pieraccini, Firenze, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Bani
authors.forename: N
authors.initials: N
authors.lastname: Pimpinelli
authors.forename: S
authors.initials: S
authors.lastname: Moretti
authors.forename: B
authors.initials: B
authors.lastname: Giannotti
mesh_terms: D006801:Humans; D007801:Langerhans Cells / Q000473:pathology*; D008854:Microscopy, Electron; D009182:Mycosis Fungoides / Q000473:pathology*; D012867:Skin / Q000473:pathology* / Q000648:ultrastructure; D012878:Skin Neoplasms / Q000473:pathology* / Q000648:ultrastructure