title: Ultraviolet-absorbing organic anions in uremic serum separated by capillary zone electrophoresis, and quantification of hippuric acid.
abstract: Organic anions accumulated in blood serum of patients with chronic renal failure were separated by a novel technique: closed-system capillary zone electrophoresis (CZE) in a pH6 carrier-electrolyte system. Hippuric acid (HA), p-hydroxyhippuric acid, and uric acid were identified by their co-elution with standards prepared in ultrafiltered normal serum and by comparison with the corresponding ultraviolet-detected peaks positively identified in the HPLC analyses. Analysis time for the entire profile is 8 min. Repeatabilities (CVs) of CZE migration times and peak areas of the three acids in serum samples were about 0.7% and 6%, respectively. We quantified HA in 10 ultrafiltered uremic serum samples and compared results with those by a previously described HPLC procedure. The very good agreement further supports the identification of hippuric acid. Accuracy and precision of the CZE method were similar to those for the HPLC gradient-elution method, but analysis time for HA (8 min) is much less than by HPLC (90 min). Our technique is very suitable for selective, rapid analysis for (ultraviolet-absorbing) anionic constituents in ultrafiltered uremic serum, without any sample pretreatment.
authors.affiliation: Faculty of Chemical Engineering, Eindhoven University of Technology, The Netherlands.
authors.forename: A C
authors.initials: AC
authors.lastname: Schoots
authors.forename: T P
authors.initials: TP
authors.lastname: Verheggen
authors.forename: P M
authors.initials: PM
authors.lastname: De Vries
authors.forename: F M
authors.initials: FM
authors.lastname: Everaerts
mesh_terms: D000838:Anions; D002197:Capillary Action; D002851:Chromatography, High Pressure Liquid; D004586:Electrophoresis / Q000706:statistics & numerical data; D006626:Hippurates / Q000097:blood*; D006801:Humans; D011786:Quality Control; D006435:Renal Dialysis; D014462:Ultrafiltration; D014511:Uremia / Q000097:blood* / Q000628:therapy; D014527:Uric Acid / Q000097:blood
chemical_list: D000838:Anions; D006626:Hippurates; D014527:Uric Acid; C056283:4-hydroxyhippuric acid; C030514:hippuric acid