Effects of attachment substrates on the growth and differentiation of LLC-PK1 cells.
The growth and differentiation of an established renal epithelial cell line, LLC-PK1, on membrane bound mussel adhesive protein (MAP), collagen, and extracellular matrix (ECM) in serum-containing medium was studied. Cell attachment and growth on uncoated- vs. protein-coated cellulose nitrate and acetate membranes did not differ significantly, and confluence was achieved on all membranes. However, cells remained in a single monolayer only when plated on collagen or ECM. LLC-PK1 monolayers grown on ECM-coated membranes displayed the highest transepithelial D-glucose transport (333 +/- 22 ng.cm-2.min-1) whereas cells plated on collagen-coated membranes displayed the lowest (94 +/- 23 ng.cm-2.min-1). Glucose flux values increased with age of the culture, reaching a plateau at 28 d postseeding. These results indicate that the underlying substratum and cell age can affect differentiation of renal epithelial cells in vitro.
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Artificial Organ Laboratory, Brown University, Providence, Rhode Island 02912.
Galletti
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Aebischer
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D000375:Aging / Q000473:pathology / Q000502:physiology; D000818:Animals; D001692:Biological Transport; D002448:Cell Adhesion / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D003470:Culture Media / Q000378:metabolism; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism / Q000502:physiology; D005947:Glucose / Q000378:metabolism / Q000493:pharmacokinetics; D007444:Inulin / Q000378:metabolism; D007668:Kidney / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D008854:Microscopy, Electron; D011506:Proteins / Q000378:metabolism; D013552:Swine
D003470:Culture Media; D011506:Proteins; C037403:adhesive protein, mussel; D007444:Inulin; D005947:Glucose