title: Nociceptive effects induced by intrathecal administration of prostaglandin D2, E2, or F2 alpha to conscious mice.
abstract: The effects of intrathecal administration of prostaglandins on pain responses in conscious mice were evaluated by using hot plate and acetic acid writhing tests. Prostaglandin D2 (0.5-3 ng/mouse) had a hyperalgesic action on the response to a hot plate during a 3-60 min period after injection. Prostaglandin E2 showed a hyperalgesic effect at doses of 1 pg to 10 ng/mouse, but the effect lasted shorter (3-30 min) than that of prostaglandin D2. Similar results were obtained by acetic acid writhing tests. The hyperalgesic effect of prostaglandin D2 was blocked by simultaneous injection of a substance P antagonist (greater than or equal to 100 ng) but not by AH6809, a prostanoid EP1-receptor antagonist. Conversely, prostaglandin E2-induced hyperalgesia was blocked by AH6809 (greater than or equal to 500 ng) but not by the substance P antagonist. Prostaglandin F2 alpha had little effect on pain responses. These results demonstrate that both prostaglandin D2 and prostaglandin E2 exert hyperalgesia in the spinal cord, but in different ways.
authors.affiliation: Hayaishi Bioinformation Transfer Project, Research Development Corporation of Japan, Takatsuki.
authors.forename: R
authors.initials: R
authors.lastname: Uda
authors.forename: S
authors.initials: S
authors.lastname: Horiguchi
authors.forename: S
authors.initials: S
authors.lastname: Ito
authors.forename: M
authors.initials: M
authors.lastname: Hyodo
authors.forename: O
authors.initials: O
authors.lastname: Hayaishi
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D006930:Hyperalgesia / Q000139:chemically induced*; D006941:Hyperesthesia / Q000139:chemically induced*; D007278:Injections, Spinal; D008297:Male; D051379:Mice; D009619:Nociceptors / Q000187:drug effects* / Q000502:physiology; D011453:Prostaglandins / Q000494:pharmacology*; D013116:Spinal Cord / Q000187:drug effects* / Q000503:physiopathology; D014966:Xanthenes / Q000494:pharmacology; D044004:Xanthones
chemical_list: D011453:Prostaglandins; D014966:Xanthenes; D044004:Xanthones; C053876:6-isopropoxy-9-oxoxanthene-2-carboxylic acid