A new method for culturing human hair follicle cells on floating mixed collagen membranes.
A new method for culturing human hair follicle cells without a biological feeder layer has been developed. Dispersed hair follicle cells obtained from plucked hairs have been successfully grown on floating mixed collagen membranes (collagen types I and IV) and have reached a confluent state. They could be subcultured up to twice. Differentiation of the cells was maintained throughout a month in culture. Stratification of the cells and continuous epithelial pavement formation were seen, as in cultured epidermal keratinocytes.
Kuwana
R
R
Department of Dermatology, Kochi Red Cross Hospital, Japan.
Arase
S
S
Sadamoto
Y
Y
Takeda
K
K
D003094:Collagen; D046508:Culture Techniques / Q000379:methods*; D006197:Hair / Q000166:cytology*; D006801:Humans; D008567:Membranes, Artificial
D008567:Membranes, Artificial; D003094:Collagen