title: Optimization of an isocratic reversed phase liquid chromatographic system for the separation of fourteen steroids using factorial design and computer simulation.
abstract: An optimization strategy for an isocratic reversed phase high performance liquid chromatographic system (RP-HPLC) is described. Factorial design and a computer program are used to predict the retention time and resolution of fourteen steroids. An optimized rapid (less than 25 min) isocratic RP-HPLC system for the satisfactory separation of cortisone, cortisol, corticosterone, 11-deoxycortisol, 11-deoxycorticosterone, 17 alpha-hydroxyprogesterone, progesterone, androstenedione, testosterone, estrone, estradiol, estriol, prednisone acetate and dexamethasone acetate has been developed using this strategy through eight experiments.
authors.affiliation: Department of Internal Medicine, Affiliated Hospital of Shandong Medical University, Jinan, China.
authors.forename: J Q
authors.initials: JQ
authors.lastname: Wei
authors.forename: J L
authors.initials: JL
authors.lastname: Wei
authors.forename: X T
authors.initials: XT
authors.lastname: Zhou
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003198:Computer Simulation; D006801:Humans; D008432:Mathematical Computing; D013256:Steroids / Q000032:analysis*
chemical_list: D013256:Steroids