Protection against rabies in mice by a cytotoxic T cell clone recognizing the glycoprotein of rabies virus.
By the use of liposomes containing the purified surface glycoprotein (G) of rabies virus and the haemagglutinin-neuraminidase (HN) and fusion (F) glycoproteins of Sendai virus, the target antigen of anti-rabies virus cytotoxic T lymphocyte (CTL) clones isolated in a previous study was identified as the G protein. Recognition of the H-2K determinant of the class I major histocompatibility complex (MHC) was necessary for target lysis by the CTL clones. One of the CTL clones was examined for the ability to protect mice against a lethal rabies virus infection. CTL were transferred into syngeneic mice which had been infected in the hind footpad with the ERA strain of rabies virus. The infection was converted into a lethal infection by cyclophosphamide treatment 1 day after virus infection. Transfer of CTL 2 to 3 days after virus infection protected approximately 50% of mice during the observation period of 4 weeks. Greater protection was obtained in mice receiving both anti-rabies virus antibodies and CTL cells.
Kawano
H
H
Department of Microbiology, Medical College of Oita, Japan.
Mifune
K
K
Ohuchi
M
M
Mannen
K
K
Cho
S
S
Hiramatsu
K
K
Shichijo
A
A
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D007111:Immunity, Cellular; D007116:Immunization, Passive; D007165:Immunosuppression Therapy; D008081:Liposomes; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011818:Rabies / Q000517:prevention & control*; D011820:Rabies virus / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D006023:Glycoproteins; D008081:Liposomes; D014764:Viral Proteins