Glycosphingolipids of Echinococcus multilocularis metacestodes.
Neutral and acid glycosphingolipids of Echinococcus multilocularis metacestodes that were obtained after intraperitoneal infection of Meriones unguiculatus have been analyzed by thin layer chromatography. Neutral and acid glycosphingolipids accounted for 95% and 5% of total glycosphingolipids, respectively. 12 different fractions were observed in the neutral glycosphingolipids extracts of the parasite. The most important was a monohexosylceramide fraction accounting for 56.4% of neutral glycosphingolipids. 9 different fractions were detected in gangliosides (acid glycosphingolipids). The fact that these glycosphingolipids were specific to the parasite was established by the analysis of different cell populations of the host. Glycosphingolipids were purified from control and parasite-infected gerbil blood cells as well as from peritoneal exudate cells of healthy gerbils after a non-specific immunostimulation. The chromatograms obtained with these extracts were totally different from the parasite. In addition, parasitosis was found to have no effect on the host blood cell glycosphingolipids.
Persat
F
F
Département de Parasitologie et Pathologie Exotique, Université Claude Bernard, Lyon 1, France.
Bouhours
J F
JF
Mojon
M
M
Petavy
A F
AF
D000818:Animals; D001773:Blood Cells / Q000032:analysis / Q000469:parasitology; D002469:Cell Separation; D002855:Chromatography, Thin Layer; D004443:Echinococcosis / Q000097:blood; D004446:Echinococcus / Q000032:analysis*; D005849:Gerbillinae; D006028:Glycosphingolipids / Q000032:analysis* / Q000097:blood
D006028:Glycosphingolipids