Specificities of monoclonal antibodies against the complement C3d split product.
The only specific method at present for the quantification of the complement split product C3d, the double-decker rocket immunoelectrophoretic assay, is work intensive and expensive. We investigated the possibilities for developing an ELISA for the direct quantification of C3d in plasma. Available antibodies were examined for their specificity for C3 and its degradation products by the PAGE immunoblotting method. None of the antibodies were specific for C3d, as cross-reactivities against C3 and/or C3b and C3bi were demonstrable.
Lund
E D
ED
Department of Clinical Chemistry, Vejle County Hospital, Denmark.
Sørensen
L S
LS
Brandslund
I
I
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D015933:Complement C3d / Q000276:immunology* / Q000378:metabolism; D003429:Cross Reactions / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D008567:Membranes, Artificial; D011145:Polyvinyls; D011817:Rabbits; D051381:Rats; D012967:Sodium Dodecyl Sulfate; D005465:Fluorocarbon Polymers
D000911:Antibodies, Monoclonal; D015933:Complement C3d; D008567:Membranes, Artificial; D011145:Polyvinyls; D012967:Sodium Dodecyl Sulfate; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers