title: Polyunsaturated fatty acid profiles and alpha-tocopherol levels in plasma and whole blood incubated with copper. Evidence of inhibition of lipoperoxidation in plasma by hemolysate.
abstract: Polyunsaturated fatty acid (PUFA) profiles and alpha-tocopherol levels were studied in human plasma and whole blood incubated with copper under air or nitrogen. In plasma, both PUFAs and alpha-tocopherol disappeared. The results were completely different in whole blood: (i) in plasma, while alpha-tocopherol decreased in the same manner as in plasma incubated alone, profiles of PUFA were only slightly modified. So, in spite of the absence of alpha-tocopherol, lipoperoxidation was not very marked. That is why the release of a protective factor from erythrocytes during hemolysis was under consideration. This was confirmed by the complete inhibition of degradation of PUFAs in plasma when hemolysate was added; (ii) In erythrocytes, no modification in PUFA profiles could be detected while alpha-tocopherol decreased slightly. Thus, not only do erythrocytes resist the copper-dependent oxidative stress in an incredible manner, but they also seem to protect plasma at the time of hemolysis.
authors.affiliation: Laboratoire de Biochimie et de Toxicologie, Faculté de Médecine et de Pharmacie, Poitiers, France.
authors.forename: S
authors.initials: S
authors.lastname: Chahboun
authors.forename: C
authors.initials: C
authors.lastname: Tallineau
authors.forename: R
authors.initials: R
authors.lastname: Pontcharraud
authors.forename: A
authors.initials: A
authors.lastname: Guettier
authors.forename: A
authors.initials: A
authors.lastname: Piriou
mesh_terms: D000328:Adult; D000388:Air; D003300:Copper / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000097:blood*; D006461:Hemolysis; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D009584:Nitrogen; D014810:Vitamin E / Q000097:blood*
chemical_list: D005231:Fatty Acids, Unsaturated; D014810:Vitamin E; D003300:Copper; D009584:Nitrogen