Synchronous fluorescence spectroscopic, immunoaffinity chromatographic and 32P-postlabeling analysis of human placental DNA known to contain benzo[a]pyrene diol epoxide adducts.
Human placenta readily catalyzes the biotransformation of polycyclic aromatic hydrocarbons (PAHs) and other carcinogens to reactive metabolites that can damage DNA through formation of covalent adducts. Placenta is widely available for epidemiologic studies and may be a useful dosimeter for carcinogen exposures in humans. However, previous studies of human placental DNA have yielded discrepant results with respect to PAH-DNA adducts. In order to resolve some of the issues surrounding these discrepancies, placental DNA samples known to contain benzo[a]pyrene diol epoxide adducts were also analyzed by 32P-postlabeling and immunoaffinity chromatography. Results indicate that previous discrepancies can be accounted for by methodologic factors affecting the specificities of adduct assays in biological samples and suggest that human placental DNA contains adducts derived from multiple PAHs.
Manchester
D K
DK
Department of Pediatrics, University of Colorado Health Sciences Center, Denver 80218.
Wilson
V L
VL
Hsu
I C
IC
Choi
J S
JS
Parker
N B
NB
Mann
D L
DL
Weston
A
A
Harris
C C
CC
D001564:Benzo(a)pyrene / Q000032:analysis*; D001580:Benzopyrenes / Q000032:analysis*; D002846:Chromatography, Affinity; D004247:DNA / Q000032:analysis*; D018736:DNA Adducts; D004249:DNA Damage; D005260:Female; D006801:Humans; D010761:Phosphorus Radioisotopes; D010920:Placenta / Q000032:analysis*; D013050:Spectrometry, Fluorescence
D001580:Benzopyrenes; D018736:DNA Adducts; D010761:Phosphorus Radioisotopes; C056612:benzo(a)pyrene-DNA adduct; D001564:Benzo(a)pyrene; C006707:benzo(a)pyrene 4,5-epoxide; D004247:DNA