Characterization of a reversible denaturational transition occurring in the case of an irreversibly denaturing membrane spanning protein.
During a previous investigation of the thermal denaturation of the membrane spanning domain of the human erythrocyte band 3 protein, a novel transition was noted. The most significant aspect of this transition is its reversibility, since both the endothermic and the functional denaturation of band 3 are clearly irreversible. In this report this reversible thermal transition, manifested by a change in the temperature course of the heat capacity at the protein thermodenaturation temperature, is characterized and briefly discussed.
Maneri
L R
LR
Department of Chemistry, Purdue University, West Lafayette, IN 47907.
D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism*; D002152:Calorimetry, Differential Scanning; D006801:Humans; D007700:Kinetics; D011489:Protein Denaturation; D014357:Trypsin
D001457:Anion Exchange Protein 1, Erythrocyte; D014357:Trypsin