title: Determination of Senecio alkaloids by thermospray liquid chromatography/mass spectrometry.
abstract: A series of Senecio alkaloid and alkaloid N-oxide standards has been analyzed using positive and negative ion thermospray liquid chromatography/mass spectrometry (LC/MS) with an ammonium acetate-containing mobile phase. On-line separations of pyrrolizidine alkaloids from extracts of Senecio jacobaea (tansy ragwort) and Senecio vulgaris (common groundsel) were done using an ammonium hydroxide-containing mobile phase. All of the alkaloids known to be present in the extracts were detected by ammonium hydroxide thermospray LC/MS, as well as many other components which may be as-yet-unidentified alkaloids.
authors.affiliation: Laboratory of Molecular Biophysics, NIEHS, Research Triangle Park, North Carolina 27709.
authors.forename: C E
authors.initials: CE
authors.lastname: Parker
authors.forename: S
authors.initials: S
authors.lastname: Verma
authors.forename: K B
authors.initials: KB
authors.lastname: Tomer
authors.forename: R L
authors.initials: RL
authors.lastname: Reed
authors.forename: D R
authors.initials: DR
authors.lastname: Buhler
mesh_terms: D000085:Acetates; D000470:Alkaloids / Q000032:analysis*; D064753:Ammonium Hydroxide; D002851:Chromatography, High Pressure Liquid; D006878:Hydroxides; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000032:analysis; D010947:Plants, Toxic; D011763:Pyrrolizidine Alkaloids / Q000032:analysis / Q000302:isolation & purification; D012674:Senecio / Q000032:analysis*; D012997:Solvents
chemical_list: D000085:Acetates; D000470:Alkaloids; D006878:Hydroxides; D010936:Plant Extracts; D011763:Pyrrolizidine Alkaloids; D012997:Solvents; D064753:Ammonium Hydroxide; C018824:ammonium acetate