Transforming growth factor-beta 1 is a costimulator for IgA production.
Transforming growth factor-beta 1 (TGF-beta 1) belongs to a family of polypeptides involved in the regulation of cell growth and differentiation. We have examined the ability of TGF-beta 1 to regulate isotype specific Ig secretion by murine spleen B cells. TGF-beta 1, in the presence of rIL-2, induced a synergistic 10-fold or greater increase in IgA secretion by LPS-stimulated spleen B cells. TGF-beta 1 alone had little to no effect on IgA secretion. In contrast, TGF-beta 1, with or without rIL-2, markedly inhibited IgG1 and IgM secretion under the same conditions. The costimulatory activity of TGF-beta 1 and rIL-2 on IgA secretion was seen in cultures of surface IgA negative B cells and was inhibited by anti-TGF-beta 1 antibody in a dose dependent manner. Vicia villosa agglutinin non-adherent Peyer's patch T cells, which secrete IL-2, also synergized with TGF-beta 1 and could substitute for the activity of LPS and rIL-2 on the IgA response. Finally, IL-5 added after 2 days of culture, but not at the beginning of culture, synergized with TGF-beta 1 on the IgA response. These studies indicate that TGF-beta 1 can interact with other lymphokines and selectively modulate the IgA response.
Kim
P H
PH
Department of Medicine, University of California, San Diego, La Jolla 92093.
Kagnoff
M F
MF
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D007070:Immunoglobulin A / Q000096:biosynthesis*; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000494:pharmacology; D015848:Interleukin-5 / Q000494:pharmacology; D037102:Lectins / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D037121:Plant Lectins; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism; D011994:Recombinant Proteins; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology; D015291:Transforming Growth Factors / Q000494:pharmacology*
D007070:Immunoglobulin A; D007376:Interleukin-2; D015848:Interleukin-5; D037102:Lectins; D008070:Lipopolysaccharides; D037121:Plant Lectins; D011947:Receptors, Antigen, B-Cell; D011994:Recombinant Proteins; C042242:Vicia lectins; D015291:Transforming Growth Factors