title: Chemical synthesis of bovine transforming growth factor-alpha: synthesis, characterization and biological activity.
abstract: Bovine transforming growth factor-alpha (bTGF-alpha) is a 50 amino acid polypeptide with three disulfide linkages. In order to evaluate the biological function of this peptide, bTGF-alpha was synthesized via an automatic synthesizer and purified to homogeneity in high yield. The integrity of this synthetic peptide was confirmed by chemical analyses and bioassays. In a bovine liver radioreceptor assay, bTGF-alpha competes with radiolabeled EGF and has activity comparable to mEGF and hTGF-alpha. Compared to hEGF, bTGF-alpha elicits a greater response in a bovine mammary cell proliferation.
authors.affiliation: Monsanto Company, St. Louis, Missouri 63198.
authors.forename: J S
authors.initials: JS
authors.lastname: Tou
authors.forename: M F
authors.initials: MF
authors.lastname: McGrath
authors.forename: M E
authors.initials: ME
authors.lastname: Zupec
authors.forename: J C
authors.initials: JC
authors.lastname: Byatt
authors.forename: B N
authors.initials: BN
authors.lastname: Violand
authors.forename: L A
authors.initials: LA
authors.lastname: Kaempfe
authors.forename: B D
authors.initials: BD
authors.lastname: Vineyard
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D066246:ErbB Receptors / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000166:cytology; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D011487:Protein Conformation; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D015291:Transforming Growth Factors / Q000138:chemical synthesis* / Q000494:pharmacology
chemical_list: D007202:Indicators and Reagents; D015291:Transforming Growth Factors; D066246:ErbB Receptors