Glycoprotein synthesis in the subcommissural organ of the chick embryo. II. An immunochemical study.
In the chick embryo, A74 immunoaffinity chromatography allowed to purify specific glycoproteins relevant to the SCO ventricular secretory process. The eluted fractions of the subcommissural organ (SCO), the cerebral hemispheres (CH) and the medulla oblongata (MO) were compared using the Concanavalin A (Con A) and wheat germ agglutinin (WGA) staining procedures after western-blotting. Analysis of the optical density of the reactive bands allowed to estimate the relative concentration of the various glycopeptides in the eluted fractions. In the SCO-eluted fractions at least ten Con A-positive glycopeptides were identified, their apparent molecular weight ranging from 240 to 42 kD. Only three of these appeared to be WGA-positive (98, 88, and 52 kD). In the CH-eluted fractions only a 52 kD Con A- and WGA-positive glycopeptide was revealed, while in the MO-eluted fractions a 32 kD glycopeptide was also Con A- and WGA-positive. These results are discussed in regard to the known biosynthesis pathway of complex type glycoproteins.
Karoumi
A
A
Laboratoire de Biologie animale, Université Blaise Pascal, Clermont II, Aubière, France.
Croisille
Y
Y
Croisille
F
F
Meiniel
R
R
Belin
M F
MF
Meiniel
A
A
D000818:Animals; D002642:Chick Embryo; D002846:Chromatography, Affinity; D003208:Concanavalin A / Q000378:metabolism; D005314:Embryonic and Fetal Development; D006023:Glycoproteins / Q000096:biosynthesis*; D008970:Molecular Weight; D009490:Neurosecretory Systems / Q000378:metabolism*; D013351:Subcommissural Organ / Q000196:embryology / Q000378:metabolism*
D006023:Glycoproteins; D003208:Concanavalin A