Amino acid sequence of ghilanten: anticoagulant-antimetastatic principle of the South American leech, Haementeria ghilianii.
This study reports the amino acid sequence of ghilanten, an anticoagulant-antimetastatic principle of the hematophagous leech, Haementeria ghilianii. Ghilanten consists of 119 amino acids with twenty cysteines and a consensus sequence for heparin-binding at its carboxyl-terminus. Arginine-34 represents the reactive residue involved in the active-site inhibition of trypsin and Factor Xa. Immunoreactivity data suggest that heterogeneity among ghilantens is due in part to amino acid substitutions at their carboxyltermini.
Blankenship
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Merrell Dow Research Institute, Cincinnati, OH 45215.
Brankamp
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Manley
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Cardin
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AD
D000595:Amino Acid Sequence; D000818:Animals; D000925:Anticoagulants; D000970:Antineoplastic Agents; D002851:Chromatography, High Pressure Liquid; D007447:Invertebrate Hormones / Q000302:isolation & purification; D007865:Leeches; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D012469:Salivary Glands; D012689:Sequence Homology, Nucleic Acid
D000925:Anticoagulants; D000970:Antineoplastic Agents; D007447:Invertebrate Hormones; C063005:ghilanten