title: SIMFIT: a microcomputer software-toolkit for modelistic studies in biochemistry.
abstract: A software package suitable for personal computers and designed to handle simulation and fitting problems related to the study of biomolecules under pre-steady and steady state conditions is presented, and its overall architecture as well as the implemented algorithms illustrated. The peculiar features of the package are: (i) integrated capability of simulating dynamic models and fitting to them experimental data; (ii) handling of stiff problems; (iii) free use of algebraic as well as differential equations; (iv) objective comparison of models of different complexity. The above features are discussed through a number of examples taken from the direct experience of the authors in enzyme kinetics and ligand binding.
authors.affiliation: Institute of Biochemistry, Humboldt University, Berlin, GDR.
authors.forename: H G
authors.initials: HG
authors.lastname: Holzhütter
authors.forename: A
authors.initials: A
authors.lastname: Colosimo
mesh_terms: D001669:Biochemical Phenomena; D001671:Biochemistry; D003198:Computer Simulation; D004798:Enzymes / Q000378:metabolism; D007700:Kinetics; D008024:Ligands; D008838:Microcomputers; D008956:Models, Chemical; D012984:Software
chemical_list: D004798:Enzymes; D008024:Ligands