title: Investigation of ecdysteroid excretion by adult Dirofilaria immitis and Brugia pahangi.
abstract: The excretion of ecdysteroids by the filarial nematode species, Dirofilaria immitis and Brugia pahangi, was examined both in vitro, by the analysis of culture medium, and in vivo, through analysis of serum samples from experimentally infected hosts. There was no evidence of ecdysteroid excretion by intact parasites of either species in vitro. Free ecdysteroids were detected in the serum of ferrets and dogs infected with D. immitis, but concentrations would be at or below the limit of detection in sub-millilitre serum samples. The detection of ecdysteroids in the serum of potential hosts is unlikely to be of value in the diagnosis of filarial infections due to a combination of low titre in the presence of current infection and measurable titre in its absence. Ecdysteroids of dietary origin may contribute to the latter.
authors.affiliation: Department of Biochemistry, University of Liverpool, U.K.
authors.forename: J G
authors.initials: JG
authors.lastname: Mercer
authors.forename: G C
authors.initials: GC
authors.lastname: Barker
authors.forename: R E
authors.initials: RE
authors.lastname: Howells
authors.forename: H H
authors.initials: HH
authors.lastname: Rees
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D002009:Brugia / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000032:analysis; D004183:Dirofilaria immitis / Q000378:metabolism*; D004184:Dirofilariasis / Q000175:diagnosis* / Q000662:veterinary; D004283:Dog Diseases / Q000175:diagnosis; D004285:Dogs; D026461:Ecdysteroids; D005260:Female; D005289:Ferrets; D005370:Filarioidea / Q000378:metabolism*; D066298:In Vitro Techniques; D007447:Invertebrate Hormones / Q000097:blood / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism; D011863:Radioimmunoassay
chemical_list: D003470:Culture Media; D026461:Ecdysteroids; D007447:Invertebrate Hormones