Increased immunogenicity of a non-amphipathic protein (BSA) after inclusion into iscoms.
Bovine serum albumin (BSA) was used as a non-amphipathic model protein to be included into iscoms. Pretreatment at an acidic pH (2.5) was used to reveal hydrophobic regions, after which BSA could be integrated. In immunization experiments in mice the BSA iscoms induced long lasting and considerably higher serum antibody responses than non-treated monomeric BSA or BSA aggregated by acidic treatment.
Morein
B
B
Swedish University of Agricultural Sciences, College of Veterinary Medicine, Department of Veterinary Microbiology, Uppsala, BMC.
Ekström
J
J
Lövgren
K
K
D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006863:Hydrogen-Ion Concentration; D007114:Immunization; D051379:Mice; D011487:Protein Conformation; D012710:Serum Albumin, Bovine / Q000276:immunology*
D000941:Antigens; D012710:Serum Albumin, Bovine