title: Amino acid sequence of ghilanten: anticoagulant-antimetastatic principle of the South American leech, Haementeria ghilianii.
abstract: This study reports the amino acid sequence of ghilanten, an anticoagulant-antimetastatic principle of the hematophagous leech, Haementeria ghilianii. Ghilanten consists of 119 amino acids with twenty cysteines and a consensus sequence for heparin-binding at its carboxyl-terminus. Arginine-34 represents the reactive residue involved in the active-site inhibition of trypsin and Factor Xa. Immunoreactivity data suggest that heterogeneity among ghilantens is due in part to amino acid substitutions at their carboxyltermini.
authors.affiliation: Merrell Dow Research Institute, Cincinnati, OH 45215.
authors.forename: D T
authors.initials: DT
authors.lastname: Blankenship
authors.forename: R G
authors.initials: RG
authors.lastname: Brankamp
authors.forename: G D
authors.initials: GD
authors.lastname: Manley
authors.forename: A D
authors.initials: AD
authors.lastname: Cardin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000925:Anticoagulants; D000970:Antineoplastic Agents; D002851:Chromatography, High Pressure Liquid; D007447:Invertebrate Hormones / Q000302:isolation & purification; D007865:Leeches; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D012469:Salivary Glands; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000925:Anticoagulants; D000970:Antineoplastic Agents; D007447:Invertebrate Hormones; C063005:ghilanten