Relation of Ca++ accumulation and lipid peroxidation with CCl4-induced toxicity in the rat liver.
The relationship between lipid peroxidation and Ca++ content in liver and hepatic cellular disruption was studied in 24 hr-fasted rats. Microsomal lipid peroxidation of livers in rats treated with a single dose of 16 mg or 96 mg CCl4/kg was significantly increased by 22% or 49% as measured by the diene conjugation technique. Ca++ content of liver microsomes in rats treated with 96 mg CCl4/kg was significantly increased by 20%, while the Ca++ content in rats treated with 16 mg CCl4/kg was not changed. On the other hand mitochondrial Ca++ content was tremendously increased in both rats treated with 16 mg and 96 mg CCl4/kg. Furthermore, when the activity of plasma glutamic pyruvic transaminase (GPT), which is a liver-specific enzyme, was investigated 3 hr after CCl4 treatment, hepatotoxicity was evident in rats treated with 96 mg/kg but not evident in rats treated with 16 mg/kg. These results indicate that there was a good correlation between the microsomal Ca++ content and the hepatic cellular disruption but no correlation between the microsomal lipid peroxidation and the hepatic cellular disruption as measured by the diene conjugation.
Yamamoto
H
H
Department of Environmental Medicine, University of Tsukuba, Ibaraki, Japan.
D000410:Alanine Transaminase / Q000097:blood / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002252:Carbon Tetrachloride Poisoning / Q000201:enzymology / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D000410:Alanine Transaminase; D002118:Calcium