Inhibition of protein kinase C prevents phorbol ester- but not muscarine-induced depolarizations in the rat superior cervical ganglion.
The role of protein kinase C (PKC) activation in mediating muscarinic depolarization was assessed in the rat superior cervical ganglion. Staurosporine, an inhibitor of PKC, abolished a depolarization elicited by the direct PKC activator beta-phorbol 12,13-dibutyrate, but had little effect on the response to muscarine. Thus, activation of PKC may not be an obligatory transduction step between muscarinic receptor stimulation and depolarization.
Grove
E A
EA
Department of Pharmacology, University College, London, U.K.
Caulfield
M P
MP
Evans
F J
FJ
D000200:Action Potentials / Q000187:drug effects; D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005728:Ganglia, Sympathetic / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D009116:Muscarine / Q000494:pharmacology*; D010703:Phorbol Esters / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000502:physiology*; D019311:Staurosporine
D000470:Alkaloids; D004791:Enzyme Inhibitors; D010703:Phorbol Esters; D011976:Receptors, Muscarinic; D009116:Muscarine; D011493:Protein Kinase C; D019311:Staurosporine