title: Immunohistochemical profile of cutaneous B-cell lymphoma on cryostat and paraffin sections.
abstract: Thirty cases of primary (23 cases) and secondary (seven cases) cutaneous B-cell lymphoma (CBCL) were studied by immunohistochemistry using a selected monoclonal antibody (MoAb) panel on both cryostat and paraffin sections. On cryostat sections all CBCL so tested were positive for surface membrane immunoglobulins (IgMk most often) and B-cell antigens (CD22+, CD37+) with a variable T-cell-reactive component identified by MoAbs against T-cell antigens (CD2, CD3, CD4, CD5, CD8). CD4-positive stromal T-cells were usually more numerous than CD8-positive cells. A strong (50-75% of total cells) stromal T-cell (CD2+, CD3+) reaction was found in centroblastic-centrocytic lymphoma. Small numbers of CD1+ Langerhans cells were found in most cases, but they were present in large numbers in follicular lymphoma. On paraffin sections, a combination of MoAbs against B-associated antigens (LN-1, MB2) identified B-cell lineage in virtually all cases of CBCL. CBCL was negative for MoAbs against T-associated antigens (MT1, UCHL1) with rare exceptions (two cases). However, MT1 and UCHL1 combined identified the T-cell nature of all cases of nonepidermotropic, nonmycosis T-cell lymphoma, which were initially predictive of B-lineage by histologic pattern.
authors.affiliation: Department of Anatomical Pathology, CHU Purpan, Toulouse, France.
authors.forename: P
authors.initials: P
authors.lastname: Faure
authors.forename: S
authors.initials: S
authors.lastname: Chittal
authors.forename: B
authors.initials: B
authors.lastname: Gorguet
authors.forename: P
authors.initials: P
authors.lastname: Caveriviere
authors.forename: P
authors.initials: P
authors.lastname: Brousset
authors.forename: R
authors.initials: R
authors.lastname: Viraben
authors.forename: C
authors.initials: C
authors.lastname: Mazerolles
authors.forename: G
authors.initials: G
authors.lastname: Delsol
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes; D005260:Female; D005615:Freezing; D006801:Humans; D007150:Immunohistochemistry; D008223:Lymphoma / Q000276:immunology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010232:Paraffin; D012878:Skin Neoplasms / Q000276:immunology* / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D010232:Paraffin