Evidence for idiotypic- and antiidiotypic B-B cellular interaction with the use of cloned antiidiotypic B cell line.
Immunization of BALB/c mice with MOPC104E myeloma protein induces antiidiotypic B lymphocytes that have Id-specific enhancing activity on antibody production. The B-B cell interaction was restricted to both Igh and class II MHC. However, anti-Thy-1 and C-treated splenic B cells were maintained for more than 1 y in a mixture of Con A-stimulated splenocyte culture supernatant and synthetic medium. In applying the long term culture method, we have established a cloned B cell line named B19-1d, B19-1d cells are specific to MOPC104E or J558 cross-reactive Id and they express surface mu, lambda but no Ly-1. B19-1d do not spontaneously secrete Ig but produce them upon stimulation with bacterial LPS. The effect of B19-1d cell line on idiotypic antibody production was tested. Addition of only 10 to 100 B19-1d cells into dextran-immune B cell culture greatly enhanced the Id+ antidextran antibody responses. On the contrary, the antidextran antibody production was suppressed by the higher doses of B19-1d cells. The effective cooperation between dextran-immune B cells and B19-1d cloned B cells was restricted to class II MHC. The role of idiotypic- and antiidiotypic B-B cell interaction in immune regulation and repertoire generation was suggested.
Bitoh
S
S
Department of Immunology, Kochi Medical School, Japan.
Fujimoto
S
S
Yamamoto
H
H
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000917:Antibody Formation; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D003429:Cross Reactions; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008211:Lymphocyte Cooperation; D051379:Mice; D011947:Receptors, Antigen, B-Cell / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000949:Histocompatibility Antigens Class II; D007130:Immunoglobulin Idiotypes; D008070:Lipopolysaccharides; D011947:Receptors, Antigen, B-Cell