Morphometric analysis of human chromosome satellites and NOR asymmetries by transmission electron microscopy.
Human chromosome satellites appear as roughly spherical telomeric structures of 0.215 +/- 0.013 microns mean diameter by electron microscopy. Morphometric evaluations showed that in the short arms of D and G chromosomes lacking secondary constrictions, the chromatin which constituted the satellites appeared virtually integrated within the short arms. Asymmetry was detected in sister satellites from almost identical dimensions to the near absence of one of them. When Ag-NOR staining was employed to locate active nucleolar organizer regions (NORs) these appeared associated with satellite short arms and active NORs were never found in non-satellite chromosomes. Asymmetry was also evident between sister NORs.
Malheiro
I
I
Department of Genetics, Instituto Ciências Biomédicas Abel Salazar, Porto, Portugal.
Porto
B
B
Goyanes
V
V
D004247:DNA / Q000648:ultrastructure*; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure*; D008854:Microscopy, Electron; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*
D004247:DNA