Genetic differences in the duration of the lymphocyte heat shock response in mice.
Lymphocytes from adult mice bearing a known difference in genetic susceptibility to teratogen-induced exencephaly (SWV/SD, and DBA/2J) were evaluated for changes in protein synthesis following an in vivo heat treatment. Particular attention was paid to changes indicative of the heat shock response, a highly conserved response to environmental insult consisting of induction of a few, highly conserved proteins with simultaneous decreases in normal protein synthesis. The duration of heat shock protein induction in lymphocytes was found to be increased by 1 hr in the teratogen-sensitive SWV/SD strain as compared to the resistant DBA/2J strain. Densitometric analysis revealed a significant decrease in the relative synthesis of at least two non-heat shock proteins (36 kD and 45 kD) in the SWV/SD lymphocytes as compared to DBA/2J cells. The increased sensitivity of protein synthesis to hyperthermia in the SWV/SD lymphocytes were lost in the F1 progeny of reciprocal crosses between SWV/SD and DBA/2J mouse strains. Sensitivity to hyperthermia-induced exencephaly is recessive to resistance in these crosses. The relationship between altered protein synthesis and teratogen susceptibility is discussed.
Mohl
V K
VK
Program in Genetics and Cell Biology, College of Veterinary Medicine, Washington State University, Pullman 99164-6520.
Bennett
G D
GD
Finnell
R H
RH
D000818:Animals; D003720:Densitometry; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D008214:Lymphocytes / Q000378:metabolism*; D051379:Mice; D008811:Mice, Inbred DBA; D008815:Mice, Inbred Strains; D013997:Time Factors
D006360:Heat-Shock Proteins