Lipolytic enzymes of the human pancreas. III. Auxiliary function of lipase in the cholesterol-esterase-dependent oral test on exocrine pancreatic output.
In the oral exocrine pancreatic function test using fluorescein dilaurate, this synthetic substrate attaches primarily to the triglyceride surfaces of the neutral lipids administered as part of the breakfast: these fluorescein dilaurate molecules cannot be attacked by cholesterol esterase. In the course of triglyceride saponification by lipase and colipase, however, the fluorescein dilaurate is liberated and hydrolyzed by cholesterol esterase. The pancreatic function test, therefore, measures the lipolytic activities not merely of cholesterol esterase, but indirectly of lipase, as well.
Meyer
J G
JG
Zentrallabor des Stadtkrankenhauses Hanau, Universität Marburg.
Renczes
J G
JG
Kaffarnik
H
H
D001647:Bile Acids and Salts / Q000502:physiology; D002265:Carboxylic Ester Hydrolases / Q000502:physiology*; D003089:Colipases / Q000502:physiology; D004041:Dietary Fats / Q000378:metabolism; D005452:Fluoresceins; D006801:Humans; D008049:Lipase / Q000502:physiology*; D008066:Lipolysis / Q000502:physiology*; D010179:Pancreas / Q000201:enzymology*; D010186:Pancreatic Function Tests / Q000379:methods*; D002787:Sterol Esterase / Q000502:physiology*; D014280:Triglycerides / Q000378:metabolism
D001647:Bile Acids and Salts; D003089:Colipases; D004041:Dietary Fats; D005452:Fluoresceins; D014280:Triglycerides; C034833:fluorescein dilaurate; D002265:Carboxylic Ester Hydrolases; D002787:Sterol Esterase; D008049:Lipase