title: Nicotine conditions place preferences after intracerebral administration in rats.
abstract: A single-trial place conditioning procedure, one treatment and one non-treatment during two daily conditioning sessions followed by a single test session on the 3rd day, was used to examine the place conditioning effects of intracerebrally administered nicotine. In the first series of experiments, Sprague-Dawley male rats were implanted unilaterally with guide cannulas aimed at the lateral ventricle. After 1 week, rats received either "treatment" (nicotine in 2 microliters phosphate buffer or 2 microliters of buffer alone) or "no treatment" (no injections) before being placed in the black or white compartment of a three-compartment place-conditioning apparatus for 20 min. The next day the rats received the opposite treatment before being conditioned in the opposite compartment. On day 3, animals had free access to the entire apparatus for 15 min and the time spent in each compartment was recorded automatically. Even though the rats exhibited a baseline bias for the black compartment, intracerebroventricular nicotine induced positive place preferences relative to buffer control, i.e. if treatments were paired with the black compartment, nicotine enhanced the preference for the black compartment, and if the treatments were paired with the white compartment, nicotine induced a preference for the white compartment. In addition, the nicotine-induced preference response was antagonized by the co-intraventricular administration of mecamylamine. In a second series of experiments, animals were implanted unilaterally with guide cannulas aimed at the pendunculopontine tegmental nucleus of the mesopontine tegmentum. Nicotine microinjection, 1.2-18.5 nmol in 0.5 microliter buffer, induced a dose-dependent positive place preference response.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Pharmacology, University of Kentucky College of Medicine, Lexington 40536.
authors.forename: E T
authors.initials: ET
authors.lastname: Iwamoto
mesh_terms: D000818:Animals; D001345:Autoradiography; D002755:Choice Behavior / Q000187:drug effects*; D007150:Immunohistochemistry; D007276:Injections, Intraventricular; D008297:Male; D008464:Mecamylamine / Q000494:pharmacology; D008845:Microinjections; D009538:Nicotine / Q000008:administration & dosage / Q000494:pharmacology*; D011149:Pons; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D008464:Mecamylamine; D009538:Nicotine