Botulinic toxin detection by counterimmunoelectrophoresis.
A counterimmunoelectrophoretic (CIE) technique was developed to detect botulinic toxin type A, and the method was compared with the mouse bioassay. A 100 LD50 concentration was detected within 2 h. Crossed reactions were observed with antitoxins of types B and F. As regards other Clostridium species, precipitin lines were seen between C. sporogenes and antitoxin type A. Foodstuffs artificially contaminated with C. botulinum type A were assayed by means of CIE and mouse bioassay, without recording interference by substances normally contained in foodstuffs.
Nuñez
L
L
Orientación Higiene y Sanidad, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
Amato de Lagarde
E A
EA
d'Aquino
M
M
D000818:Animals; D001681:Biological Assay; D001905:Botulinum Toxins / Q000032:analysis*; D003378:Counterimmunoelectrophoresis; D003429:Cross Reactions; D005506:Food Contamination / Q000032:analysis*; D006801:Humans; D051379:Mice; D011237:Predictive Value of Tests
D001905:Botulinum Toxins