Characterization of the interstitial cellular infiltrate in experimental chronic cyclosporine nephropathy.
In a recently described rodent model of chronic cyclosporine nephropathy (CCN) (consisting of irregularly distributed areas of interstitial inflammation, interstitial fibrosis, and tubular atrophy) we have characterized the interstitial inflammatory cells. Using a modified avidin-biotin peroxidase technique, kidney tissue was examined with monoclonal antibodies directed against leukocyte-common antigen (LCA), T lymphocytes, T helper and T nonhelper lymphocytes, Ia (B cell marker), and macrophages. Injured cortex from cyclosporine-treated animals demonstrated increased numbers of T helper and B lymphocytes, macrophages, and cells bearing LCA. Cytotoxic (T nonhelper) cells were scant. Non-injured areas of cortex from CsA-treated animals demonstrated only a modest increase in macrophages when compared with vehicle controls and normal rats. We conclude that CCN in rodents is characterized by an interstitial inflammatory infiltrate of T helper cells, B cells, and macrophages. The role of these cells in the pathogenesis of CCN, however, remains speculative.
Gillum
D M
DM
Department of Medicine, Baylor College of Medicine, Methodist Hospital, Houston, Texas 77030.
Truong
L
L
Tasby
J
J
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D003239:Connective Tissue Cells; D003524:Cyclosporins / Q000009:adverse effects*; D003593:Cytoplasm / Q000276:immunology; D004195:Disease Models, Animal; D007674:Kidney Diseases / Q000139:chemically induced* / Q000473:pathology*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D008297:Male; D010641:Phenotype; D051381:Rats; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D003524:Cyclosporins