Effect of vitamin E on 2-deoxy-D-glucose uptake in human fibroblast cultures.
2-Deoxy-D-glucose (2-DOG) uptake was tested in human fibroblast cultures in the presence and absence of vitamin E. Addition of 10 micrograms/ml vitamin E to the culture medium significantly reduced this uptake for 2-DOG concentrations of 0.005 to 10 mmol/liter (P less than or equal to 0.01). The decrease of 2-DOG uptake was inversely proportional to the rise in 2-DOG concentration (P less than or equal to 0.01). The presence of vitamin E reduced by 71% the average cellular level of lipid peroxides (expressed as thiobarbituric acid reactive substances) and caused a small but significant decrease in the cholesterol concentration (P less than or equal to 0.01). These last results might explain the decrease in 2-DOG uptake observed in the presence of vitamin E.
Couturier
M
M
Unité de Recherche d'Hépatologie Pédiatrique INSERM U 56 et Laboratoire Central de Biochimie, Hôpital de Bicêtre, France.
Lemonnier
F
F
Conti
M
M
Feneant-Thibault
M
M
Lemonnier
A
A
D001692:Biological Transport / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism; D003837:Deoxy Sugars / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008054:Lipid Peroxides / Q000378:metabolism; D012867:Skin / Q000166:cytology; D014810:Vitamin E / Q000494:pharmacology*
D003837:Deoxy Sugars; D008054:Lipid Peroxides; D014810:Vitamin E; D002784:Cholesterol; D003847:Deoxyglucose