In vitro effect of cilazaprilat on sodium-potassium transport systems in human erythrocytes.
The in vitro effects of cilazaprilat in a concentration range of 10 nM to 1 mM were investigated on various Na+ and K+ transport systems in human red blood cells. Cilazaprilat inhibited the anion carrier or DIDS-sensitive LiCO3(-)-influx and the bumetanide-sensitive K(+)-efflux. However, no effect of cilazaprilat on the Na+, K(+)-pump activity, the number of active Na+ pump units and on the Na+, Li(+)-countertransport activity could be demonstrated. It is suggested that through a decreased anion carrier indicating a decreased Na(+)-influx, the lower intracellular Na+ concentration observed in vivo during angiotensin-converting enzyme inhibition, can be at least partly explained.
Lijnen
P
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Department of Pathophysiology, University of Leuven, Belgium.
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects; D017315:Cilazapril / Q000031:analogs & derivatives*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008094:Lithium / Q000097:blood; D008297:Male; D011188:Potassium / Q000097:blood*; D011724:Pyridazines / Q000494:pharmacology*; D012414:Rubidium Radioisotopes; D012964:Sodium / Q000097:blood*
D000806:Angiotensin-Converting Enzyme Inhibitors; D011724:Pyridazines; D012414:Rubidium Radioisotopes; D017315:Cilazapril; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D008094:Lithium; D012964:Sodium; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D011188:Potassium; C045980:cilazaprilat