Proliferation and differentiation of erythroleukemia cell line (ELM-I-1) in response to erythropoietin and interleukin 3.
Here we describe the characterization of Epo-responsive mouse erythroleukemia cell line ELM-I-1. ELM-I-1 cells possess Epo binding sites on their membranes and differentiate into hemoglobin-positive cells when cultured in the presence of Epo. About 20% of the cells were hemoglobin-positive after a 3- to 4-day exposure to recombinant human Epo in liquid culture. Supplementation of recombinant mouse IL-3 during culture had an augmentative effect on Epo-mediated differentiation, although IL-3 alone did not induce differentiation. Both Epo and IL-3 stimulated the growth of ELM-I-1 cells, and their effects were a slightly additive. These findings indicate that ELM-I-1 cells are suitable for studying the interaction between Epo and IL-3 in erythroid differentiation at a subcellular level. ELM-I-1 may also offer a valuable bioassay system for Epo.
Shiozaki
M
M
Basic Research Laboratories, Ajinomoto Co., Inc., Kanagawa, Japan.
Itoh
K
K
Mori
K J
KJ
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004921:Erythropoietin / Q000494:pharmacology*; D007377:Interleukin-3 / Q000494:pharmacology*; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute; D007953:Leukemia, Radiation-Induced; D051379:Mice; D008809:Mice, Inbred C3H; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000166:cytology* / Q000187:drug effects
D007377:Interleukin-3; D011994:Recombinant Proteins; D004921:Erythropoietin