Further characterization, isolation and identification of the epithelial cell-surface antigen defined by monoclonal antibody AUA1.
The human epithelial antigen recognized monoclonal antibody (MAb) AUA1 has been characterized as a cell-surface glycoprotein. It has been isolated from human colonic mucosa by AUA1 affinity separation. N-terminal peptide sequence of this purified material has revealed a 17 amino acid sequence which identifies it with one of a group of similar epithelial/tumor-associated glycoproteins defined by a variety of MAbs. Using the polymerase chain reaction to map the gene encoding this antigen, our previous AUA1 antigen assignment to chromosome 2 has been confirmed.
Durbin
H
H
Imperial Cancer Research Fund, Lincoln's Inn Fields, London, UK.
Rodrigues
N
N
Bodmer
W F
WF
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis / Q000235:genetics; D001483:Base Sequence; D003106:Colon / Q000276:immunology; D006801:Humans; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D018396:Mucin-1
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D008562:Membrane Glycoproteins; D018396:Mucin-1