Novel sulfated polymers as highly potent and selective inhibitors of human immunodeficiency virus replication and giant cell formation.
Novel synthetic sulfated polymers, namely, sulfated polyvinyl alcohol (PVAS) and sulfated copolymers of acrylic acid with vinyl alcohol (PAVAS), proved to be potent and selective inhibitors of human immunodeficiency virus type 1 (HIV-1) and HIV-2 in vitro. The compounds completely inhibited HIV-1-induced cytopathogenicity in MT-4 cells and HIV-1 antigen expression in CEM cells at a concentration of 0.8 micrograms/ml. They were equally effective against HIV-2 replication. In addition, and in contrast to azidothymidine, PAVAS and PVAS suppressed HIV-1-induced giant cell (syncytium) formation, a process that may account for the depletion of T4 lymphocytes in patients with acquired immunodeficiency syndrome. PAVAS and PVAS completely blocked giant cell formation at a concentration of 4 micrograms/ml, whereas for dextran sulfate a concentration of 100 micrograms/ml was required to achieve complete inhibition of giant cell formation. As has been demonstrated previously for the sulfated polysaccharides, the mechanism of action of PAVAS and PVAS resides in the inhibition of virus adsorption to the cells.
Baba
M
M
Rega Institute for Medical Research, Katholieke Universiteit Leuven, Belgium.
Schols
D
D
De Clercq
E
E
Pauwels
R
R
Nagy
M
M
Györgyi-Edelényi
J
J
Löw
M
M
Görög
S
S
D000180:Acrylic Resins / Q000494:pharmacology*; D000327:Adsorption; D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D005453:Fluorescence; D015726:Giant Cells / Q000187:drug effects*; D015497:HIV-1 / Q000187:drug effects* / Q000502:physiology; D015498:HIV-2 / Q000187:drug effects* / Q000502:physiology; D014779:Virus Replication / Q000187:drug effects*
D000180:Acrylic Resins; D000998:Antiviral Agents; C063252:sulfated acrylic acid-vinyl alcohol copolymer; C063253:sulfated polyvinyl alcohol