title: The effect of topical dexamethasone and preoperative beta irradiation on a model of glaucoma fistulizing surgery in the rabbit.
abstract: We studied the effect of topical dexamethasone (1%) and preoperative beta irradiation on a model of glaucoma fistulizing surgery in the rabbit. Intraocular pressure and gross facility of aqueous outflow following surgery were not influenced by either treatment, although blebs persisted longer in the irradiated eyes. Steroids reduced clinically observable inflammation as well as the number of inflammatory cells identifiable by microscopy. Fibroblast production temporarily slowed, and ultra-structural examination demonstrated lipid-filled vacuoles and dilated mitochondria in these eyes. Also, the scar was thinner at 24 days. Beta irradiation delayed wound healing and the scar was thinner in the early postoperative stages, but the light microscopic appearance of the scar was unaltered at 59 days. Inflammation was more pronounced initially, with abundant fibrin in the wound. Recovery of the conjunctival epithelium was delayed. The delay in fibroblast recruitment and wound contraction, the thinner scar tissue, and the increased survival of the bleb are all factors that suggest that beta irradiation may be a useful adjunct to glaucoma surgery.
authors.affiliation: Moorfields Eye Hospital, Institute of Ophthalmology, London, England.
authors.forename: M H
authors.initials: MH
authors.lastname: Miller
authors.forename: I
authors.initials: I
authors.lastname: Grierson
authors.forename: W G
authors.initials: WG
authors.lastname: Unger
authors.forename: R A
authors.initials: RA
authors.lastname: Hitchings
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism; D001610:Beta Particles; D003907:Dexamethasone / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000648:ultrastructure; D005901:Glaucoma / Q000188:drug therapy / Q000473:pathology / Q000532:radiotherapy / Q000628:therapy*; D007429:Intraocular Pressure / Q000187:drug effects / Q000528:radiation effects; D011300:Preoperative Care; D011817:Rabbits; D014945:Wound Healing / Q000187:drug effects / Q000528:radiation effects
chemical_list: D003907:Dexamethasone