Hippuric acid and 3-carboxy-4-methyl-5-propyl-2-furanpropionic acid in serum and urine. Analytical approaches and clinical relevance in kidney diseases.
Hippuric acid (HA) and 3-carboxy-4-methyl-5-propyl-2-furanpropionic acid (FA) were determined in serum, plasma, ultrafiltrate and urine by gas chromatography (GC), high-performance liquid chromatography and GC with mass-selective detection, and the methods were compared. As determined by affinity chromatography and analysis of serum and ultrafiltrate, 0.5% of FA in serum occurs free and 99.5% is bound to albumin. In haemodialysed patients with chronic renal failure, the plasma levels of HA and FA are elevated in comparison with normal controls and hospital patients without kidney diseases: HA, 11.1 +/- 5.7 mg/dl (n = 86); FA, 1.9 +/- 1.2 mg/dl (n = 86). Gradual increases in HA in serum, depending on the creatinine concentrations, are found in non-dialysed patients with chronic renal failure. By haemodialysis and haemofiltration the HA levels are lowered (53-66 and 30-36%, respectively), whereas FA is not dialysable.
Liebich
H M
HM
Medizinische Universitätsklinik, Tübingen, F.R.G.
Bubeck
J I
JI
Pickert
A
A
Wahl
G
G
Scheiter
A
A
D002849:Chromatography, Gas / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005663:Furans / Q000097:blood* / Q000652:urine; D006440:Hemofiltration; D006626:Hippurates / Q000097:blood* / Q000652:urine; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000628:therapy / Q000652:urine; D011422:Propionates / Q000097:blood* / Q000652:urine; D012016:Reference Values; D006435:Renal Dialysis
D005663:Furans; D006626:Hippurates; D011422:Propionates; C041693:3-carboxy-4-methyl-5-propyl-2-furanpropionic acid; C030514:hippuric acid