Chromosomal in situ hybridization of a Hodgkin's disease-derived cell line (L540) using DNA probes for TCRA, TCRB, MET, and rRNA.
The Hodgkin's lymphoma-derived cell line L540, which exhibits multiple marker chromosomes and a genotype characteristic of T-cell origin, showed expression of MET oncogene. L540 was studied by in situ hybridization for chromosomal rearrangements concerning the regions where genes for TCRA and TCRB chains, for MET oncogene, and for rRNA are located. All four genes were demonstrated to be involved in the formation of marker chromosomes. Especially remarkable was the participation of rDNA-bearing regions in two different translocations.
Fonatsch
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C
Institut für Humangenetik, Medizinische Universität zu Lübeck, Federal Republic of Germany.
Gradl
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G
Kolbus
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U
Rieder
H
H
Tesch
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H
D002460:Cell Line; D002871:Chromosome Banding; D015342:DNA Probes; D005819:Genetic Markers; D006689:Hodgkin Disease / Q000235:genetics*; D006801:Humans; D007621:Karyotyping; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D012335:RNA, Ribosomal / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000235:genetics; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D005819:Genetic Markers; D012335:RNA, Ribosomal; D011948:Receptors, Antigen, T-Cell