title: Protein differences in human pancreatic cancer cell lines with diverse metastatic potential.
abstract: High-resolution two-dimensional polyacrylamide gel electrophoresis was performed on the pancreatic cell lines SG, SG-R, FG, and L3.5, which when injected into the spleen of nude mice produced hepatic metastases in 0%, 20%, 64%, and 100% of the animals, respectively. A total of 981 proteins were quantitatively identified. In the highly metastatic lines, 13 proteins were present in statistically significant greater quantities, while 4 proteins were present in statistically significant greater quantities in the cell lines with a low metastatic potential. Two proteins were unique to the highly metastatic lines, while 16 proteins were unique to the lines with a low metastatic potential. These results suggest that there are considerable quantitative and qualitative differences in the cellular proteins of human pancreatic cancer cell lines with a varying metastatic potential and imply a biochemical basis to tumor heterogeneity and metastases.
authors.affiliation: Department of Surgery, Brown University, Providence, RI.
authors.forename: M A
authors.initials: MA
authors.lastname: Schwalke
authors.forename: C M
authors.initials: CM
authors.lastname: Doremus
authors.forename: R
authors.initials: R
authors.lastname: Bleday
authors.forename: H J
authors.initials: HJ
authors.lastname: Wanebo
authors.forename: M P
authors.initials: MP
authors.lastname: Vezeridis
mesh_terms: D000818:Animals; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008970:Molecular Weight; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000378:metabolism*; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000378:metabolism
chemical_list: D009363:Neoplasm Proteins