Cytogenetic effects of near ultraviolet radiation in normal and systemic lupus erythematosus lymphocytes.
We have conducted a study on the spontaneous and UV-A-induced frequency of chromosomal breaks and sister-chromatid exchanges (SCE) in purified lymphocytes from normal donors and from systemic lupus erythematosus (SLE) patients who were in clinical remission at the time of the study. Our results show that although SLE lymphocytes exhibit a higher frequency of spontaneous SCEs than controls, the rate of chromosomal breakage is comparable in the 2 groups. In both controls and patients, irradiation with UV-A (320-400 nm) increases the SCE values but does not significantly affect the frequency of chromosomal aberrations.
Caporossi
D
D
Department of Public Health and Cellular Biology, II University, Rome, Italy.
Sebastiani
G
G
Masala
C
C
Nicoletti
B
B
D000328:Adult; D016009:Chi-Square Distribution; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000528:radiation effects; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000235:genetics*; D008214:Lymphocytes / Q000528:radiation effects* / Q000648:ultrastructure; D008297:Male; D012854:Sister Chromatid Exchange; D014466:Ultraviolet Rays