A specific base transition occurs on replicating hepatitis delta virus RNA.
Three independent lines of evidence showed that when an infectious clone of hepatitis delta virus of known sequence was used to initiate genome replication, up to 41% of the genomes were specifically mutated in the amber termination codon (UAG to UGG) for the open reading frame of the delta antigen, thereby increasing the length of the predicted protein from 195 to 214 amino acids. This change was detected only on molecules that participated in RNA-directed RNA synthesis.
Luo
G X
GX
Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
Chao
M
M
Hsieh
S Y
SY
Sureau
C
C
Nishikura
K
K
Taylor
J
J
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics; D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D004274:DNA, Recombinant / Q000378:metabolism; D005814:Genes, Viral; D005822:Genetic Vectors; D003698:Hepatitis Delta Virus / Q000235:genetics*; D035923:Hepatitis delta Antigens; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000235:genetics*; D014158:Transcription, Genetic; D014162:Transfection
D000956:Antigens, Viral; D003062:Codon; D004274:DNA, Recombinant; D035923:Hepatitis delta Antigens; D015345:Oligonucleotide Probes; D012367:RNA, Viral; C442244:hepatitis delta virus large antigen