title: Decapitation-induced changes in inositol phosphates in rat brain.
abstract: Decapitation resulted in a time-dependent production of inositol phosphates in rat brain. This production was analyzed by measuring both the radioactivity and the concentrations of inositol phosphates generated from [3H]inositol-labeled phospholipids. Both measurements produced the same time-dependent changes, including a rapid decrease in inositol 1,4,5-trisphosphate within 1.5 min, a 6-fold increase in inositol 1,4-bisphosphate to a maximum at 1.5 min, a 5-fold rise in inositol 4-monophosphate to a maximum at 2.5 min, and little change in inositol 1-monophosphate. The temporal changes in the mass and radioactivity of these compounds, together with the decrease in labeling of phosphatidylinositol 4,5-bisphosphates, support the idea that the inositol phosphates originate from the hydrolysis of phosphatidylinositol 4,5-bisphosphates and not from either the direct hydrolysis of phosphatidylinositol 4-phosphates or phosphatidylinositols.
authors.affiliation: Department of Biochemistry, University of Missouri, Columbia 65203.
authors.forename: T N
authors.initials: TN
authors.lastname: Lin
authors.forename: G Y
authors.initials: GY
authors.lastname: Sun
authors.forename: N
authors.initials: N
authors.lastname: Premkumar
authors.forename: R A
authors.initials: RA
authors.lastname: MacQuarrie
authors.forename: S R
authors.initials: SR
authors.lastname: Carter
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002545:Brain Ischemia / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism; D003655:Decerebrate State; D007294:Inositol / Q000378:metabolism; D007295:Inositol Phosphates / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D011180:Postmortem Changes; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
chemical_list: D007295:Inositol Phosphates; D007294:Inositol