title: Decreased levels of serum alpha-isoamylase prior to diabetes onset in BB rats.
abstract: The development of insulin-dependent diabetes mellitus (IDDM) includes a prodrome of autoimmunity against pancreatic beta cells. The period of subclinical islet cell disease with altered beta-cell function may be prolonged. We have determined the serum pancreatic alpha-isoamylase in both young diabetes-prone (DP) and newly diabetic BB rats to test whether changes in the pancreas prior to IDDM are reflected by this enzyme, shown to be regulated by insulin. A prospective analysis of inbred BB rats (n = 28) that later developed diabetes showed that the alpha-isoamylase at the time of onset was reduced by 19% (p less than 0.02) compared with levels observed 1 week earlier and by 30% (p less than 0.01) compared with levels 2 weeks before onset. Furthermore, when compared to age-matched diabetes-resistant (DR) BB rats in a cross-sectional study, the DP BB rats investigated in groups at 20, 30, 40, 50, 60, and 70 days of age had significantly lower (p less than 0.01) serum alpha-isoamylase already from 50 days of age, which is 2-6 weeks prior to the expected onset of diabetes. Finally, in 70-day-old cofostered DP and DR male rats with identical body weight and rates of growth, the serum alpha-isoamylase was decreased in the DP yet nondiabetic (n = 8) rats compared with the DR (n = 8) rats (p less than 0.05). Reduced levels of serum alpha-isoamylase, therefore, may reflect loss of beta cells or beta-cell function in the pancreas of diabetes-prone but not yet diabetic BB rats.
authors.affiliation: Hagedorn Research Laboratory, Gentofte, Denmark.
authors.forename: H
authors.initials: H
authors.lastname: Markholst
authors.forename: H V
authors.initials: HV
authors.lastname: Laursen
mesh_terms: D000375:Aging / Q000097:blood; D000818:Animals; D003430:Cross-Sectional Studies; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000201:enzymology*; D005260:Female; D006026:Glycoside Hydrolases / Q000097:blood*; D007517:Isoamylase / Q000097:blood*; D008297:Male; D011236:Prediabetic State / Q000097:blood / Q000201:enzymology*; D051381:Rats; D011913:Rats, Inbred BB
chemical_list: D006026:Glycoside Hydrolases; D007517:Isoamylase