title: Atherosclerosis susceptibility differences among progenitors of recombinant inbred strains of mice.
abstract: Female mice of 16 inbred mouse strains were fed an atherogenic diet for 14 weeks and were then evaluated for atherosclerotic lesions in the aorta. Strains C57BL/6, C57BR/cd, C57L, and SM were very susceptible to atherosclerosis, with lesion area/aortic cross-sections in the range of 4500 to 8000 microns 2. Strains C58 and SWR were intermediate in susceptibility, with lesion area/sections in the range of 1670 to 1690 microns 2. Strains 129, AKR, DBA/2, and BALB/c had only small lesions in the range of 20 to 350 microns 2/section; strains C3H, NZB, CBA, HRS, SJL, and A had no lesions after 14 weeks. Lesion formation in five strains was compared at several time points. Strain C57BL/6 mice developed lesions by 7 weeks, and these continued to grow until all mice had large atheromatous plaques in the aorta and coronary arteries. Strains AKR and DBA/2 also had fatty streak lesions as early as 7 or 8 weeks, but these lesions had not progressed in size by 14 weeks. Strains BALB/c and C3H, which were both resistant to lesion formation at 14 weeks, diverged from each other as time progressed. By 1 year, BALB/c mice had large lesions, but C3H mice had none. Most of the inbred strains chosen for evaluation are the progenitors of recombinant inbred sets of strains, a genetic tool that greatly facilitates the analysis of strain differences. This survey indicates seven additional recombinant inbred sets of strains whose progenitors differ in atherosclerosis susceptibility: BXD, AKXL, SWXJ, NX8, 129XB, NXSM, and B6NXAKRN. An analysis of these recombinant inbred strains may reveal additional mouse genes affecting atherosclerosis susceptibility.
authors.affiliation: Children's Hospital Oakland Research Institute, Children's Hospital Medical Center, California.
authors.forename: B
authors.initials: B
authors.lastname: Paigen
authors.forename: B Y
authors.initials: BY
authors.lastname: Ishida
authors.forename: J
authors.initials: J
authors.lastname: Verstuyft
authors.forename: R B
authors.initials: RB
authors.lastname: Winters
authors.forename: D
authors.initials: D
authors.lastname: Albee
mesh_terms: D000818:Animals; D001011:Aorta / Q000473:pathology; D001161:Arteriosclerosis / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D008076:Cholesterol, HDL / Q000097:blood; D004036:Diet, Atherogenic; D004198:Disease Susceptibility; D005260:Female; D051379:Mice; D008815:Mice, Inbred Strains; D009206:Myocardium / Q000473:pathology; D015203:Reproducibility of Results; D013045:Species Specificity; D013997:Time Factors
chemical_list: D008076:Cholesterol, HDL