title: A temperature-sensitive mutant of Newcastle disease virus defective in intracellular processing of fusion protein.
abstract: A temperature-sensitive mutant (ts3) of Newcastle disease virus was physiologically characterized. All major viral structural proteins were synthesized at the permissive (37 degrees C) and nonpermissive (42 degrees C) temperatures, but the fusion (F) glycoprotein was not cleaved at 42 degrees C. In immunocytochemical electron microscopy, the F protein was abundant in the rough endoplasmic reticulum but not in cytoplasmic membrane at 42 degrees C. Noninfectious hemagglutinating virus particles containing all major structural proteins except the F protein were released at 42 degrees C from infected cells. We concluded that the defect in ts3 resides in the intracellular processing of the F protein.
authors.affiliation: Department of Measles Virus, National Institute of Health, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Matsumura
authors.forename: Y
authors.initials: Y
authors.lastname: Futaesaku
authors.forename: S
authors.initials: S
authors.lastname: Kohno
authors.forename: A
authors.initials: A
authors.lastname: Sugiura
authors.forename: M
authors.initials: M
authors.lastname: Kohase
mesh_terms: D000818:Animals; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002642:Chick Embryo; D003673:Defective Viruses / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D008854:Microscopy, Electron; D008970:Molecular Weight; D009154:Mutation; D009522:Newcastle disease virus / Q000235:genetics*; D013696:Temperature; D014760:Viral Fusion Proteins / Q000032:analysis / Q000235:genetics*; D014771:Virion / Q000235:genetics
chemical_list: D014760:Viral Fusion Proteins