Predictive diagnosis for polycystic kidney disease using DNA markers.
Two families in which the gene for the common, autosomal dominant form of polycystic kidney disease (PKD1) was present were examined using flanking DNA markers. The 5'HVR probe detects a linked DNA marker 8 map units distal to the PKD1 gene in males and 1 unit distal to the PKD1 gene in females. The 24-1 probe detects another linked DNA marker 4 map units proximal to the PKD1 gene in males and 0.5 map units proximal to the PKD1 gene in females. When each marker is informative they can be used as a pair flanking the disease gene to follow accurately its transmission through families for presymptomatic or prenatal prediction. For an asymptomatic individual tested in one family, DNA studies reduced the 50% prior risk of carrying the disease gene to 0.006%. An affected woman in a second family was shown to be fully informative for the flanking markers. In a future pregnancy, it will be possible to modify the 50% prior fetal risk to either 0.008% or 99.99% depending on which maternal chromosome 16 is transmitted, and provided that no cross-over occurs between the flanking markers (probability, 1.5%).
Mulley
J C
JC
Adelaide Children's Hospital, North Adelaide, SA.
Haan
E A
EA
Gedeon
A K
AK
Sutherland
G R
GR
D002874:Chromosome Mapping; D004247:DNA / Q000032:analysis*; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers / Q000032:analysis; D005838:Genotype; D006801:Humans; D008297:Male; D010375:Pedigree; D007690:Polycystic Kidney Diseases / Q000175:diagnosis* / Q000235:genetics; D011110:Polymorphism, Genetic; D011237:Predictive Value of Tests; D011296:Prenatal Diagnosis; D011995:Recombination, Genetic
D005819:Genetic Markers; D004247:DNA