title: Rapid diagnosis of typhoid fever through identification of Salmonella typhi within 18 hours of specimen acquisition by culture of the mononuclear cell-platelet fraction of blood.
abstract: Detection of Salmonella typhi in blood by culture of the mononuclear cell-platelet layer was compared with other methods currently used for the diagnosis of typhoid fever. Colonies of S. typhi were present in all mononuclear cell-platelet layer-positive cultures within 18 h of plating and were identified within an additional 10 min by a coagglutination technique. In contrast, identification of all positive cultures by conventional blood culture required 3 days.
authors.affiliation: Department of Bacterial Immunology, Walter Reed Army Institute of Research, Washington, D.C. 20307-5100.
authors.forename: F A
authors.initials: FA
authors.lastname: Rubin
authors.forename: P D
authors.initials: PD
authors.lastname: McWhirter
authors.forename: D
authors.initials: D
authors.lastname: Burr
authors.forename: N H
authors.initials: NH
authors.lastname: Punjabi
authors.forename: E
authors.initials: E
authors.lastname: Lane
authors.forename: S
authors.initials: S
authors.lastname: Kumala
authors.forename: P
authors.initials: P
authors.lastname: Sudarmono
authors.forename: S P
authors.initials: SP
authors.lastname: Pulungsih
authors.forename: M
authors.initials: M
authors.lastname: Lesmana
authors.forename: P
authors.initials: P
authors.lastname: Tjaniadi
mesh_terms: D000372:Agglutination Tests; D001792:Blood Platelets / Q000382:microbiology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000382:microbiology*; D012485:Salmonella typhi / Q000302:isolation & purification*; D013048:Specimen Handling; D013997:Time Factors; D014435:Typhoid Fever / Q000175:diagnosis*