title: Collagen and elastin metabolism in hypertensive pulmonary arteries of rats.
abstract: We evaluated the processes controlling the accumulation of collagen and elastin in main pulmonary arteries of rats during an episode of hypoxic pulmonary hypertension. Explant cultures of main pulmonary arteries were incubated with [3H]proline to measure collagen and protein synthesis and percent collagen synthesis. Elastin synthesis was measured by [14C]valine incorporation into insoluble elastin. Relative collagen synthesis increased twofold (from 1.1 +/- 0.2 x 10(3) to 2.0 +/- 1.0 x 10(3) disintegrations per minute [14C]hydroxyproline/vessel/hr/mg protein), relative collagen synthesis doubled (from 2% to 4-5% of total protein synthesis), and elastin synthesis increased ninefold (from 0.4 +/- 0.2 x 10(4) to 3.6 +/- 0.6 x 10(4) dpm [14C]valine/vessel/hr/mg protein) in early hypertension. The level of pro alpha l(I) collagen RNA paralleled the relative collagen synthetic rate during the study period. Within 7 days of recovery from hypoxia, collagen and elastin contents were normal. We conclude that collagen and elastin in main pulmonary arteries are synthesized rapidly during an episode of hypoxic pulmonary hypertension and that collagen and elastin are rapidly removed from the hypertensive vessel during normoxic recovery.
authors.affiliation: Department of Medicine, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, New Brunswick 08903-0019.
authors.forename: G J
authors.initials: GJ
authors.lastname: Poiani
authors.forename: C A
authors.initials: CA
authors.lastname: Tozzi
authors.forename: S E
authors.initials: SE
authors.lastname: Yohn
authors.forename: R A
authors.initials: RA
authors.lastname: Pierce
authors.forename: S A
authors.initials: SA
authors.lastname: Belsky
authors.forename: R A
authors.initials: RA
authors.lastname: Berg
authors.forename: S Y
authors.initials: SY
authors.lastname: Yu
authors.forename: S B
authors.initials: SB
authors.lastname: Deak
authors.forename: D J
authors.initials: DJ
authors.lastname: Riley
mesh_terms: D000199:Actins / Q000235:genetics; D000818:Animals; D003094:Collagen / Q000378:metabolism*; D004549:Elastin / Q000378:metabolism*; D006973:Hypertension / Q000378:metabolism* / Q000503:physiopathology; D011347:Procollagen / Q000235:genetics; D011506:Proteins / Q000378:metabolism; D011651:Pulmonary Artery / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000199:Actins; D011347:Procollagen; D011506:Proteins; D012333:RNA, Messenger; D003094:Collagen; D004549:Elastin