Enantioselective determination of metoprolol in plasma by liquid chromatography on a silica-bonded alpha 1-acid glycoprotein column.
The enantiomers of metoprolol were determined in plasma samples after direct resolution on a silica bonded alpha 1-acid glycoprotein column. Metoprolol was extracted from plasma into a diethyl ether-dichloromethane mixture and after back extraction to dilute phosphoric acid and adjustment of pH the sample was injected on a Chiral-AGP column for separation of R- and S-metoprolol. It was possible to measure down to 2 nmol per litre plasma with a relative standard deviation of less than 15% by use of gradient elution and fluorescence detection. The analytical method was employed to study the pharmacokinetics of the metoprolol enantiomers after administration of the racemate to humans.
Persson
B A
BA
Department of Bioanalytical Chemistry, AB Hässle, Mölndal, Sweden.
Balmér
K
K
Lagerström
P O
PO
Schill
G
G
D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D008790:Metoprolol / Q000097:blood*; D009961:Orosomucoid; D013696:Temperature
D009961:Orosomucoid; D008790:Metoprolol