title: Investigations concerning the potential for using 1H NMR relaxometry or high-resolution spectroscopy of plasma as a screening test for malignant lung disease.
abstract: In 158 plasma samples, obtained from patients with lung carcinoma, lung metastases, and infectious or inflammatory lung diseases and from healthy controls, the NMR relaxation times T1 and T2 of water protons were measured at a resonance frequency of 20 MHz by pulsed NMR techniques and adjusted to a standardized total plasma protein concentration. For one-third of these samples water-suppressed 500-MHz 1H NMR spectroscopy at 37 degrees C was used (a) to determine the widths of the composite lipid methyl and methylene signals, and (b) to quantitate individual lipid methylene signal components that could be detected in resolution-enhanced spectra. In addition, hematological parameters and the plasma levels of several acute phase proteins and apolipoprotein-A were monitored. No diagnostically significant differences between lung carcinoma patients and patients with nonmalignant lung disease could be found for any of the plasma NMR parameters, nor could T1 or lipid linewidth data distinguish between any patient group and healthy controls. However, the mean T2 was significantly shortened by about 15% for any kind of lung disease compared to healthy controls. Similar but less significant results were found for apolipoprotein-A levels. A linear discriminant function, calculated from the apolipoprotein-A and T2 data, did not improve the differentiation between malignant and nonmalignant lung disease but did improve the discrimination between tumor patients and healthy controls up to a sensitivity and specificity of 80 and 96.5%, respectively. T2 correlates inversely with plasma fibrinogen levels and the blood sedimentation rate and, therefore, appears to monitor a general inflammatory status of a tumor patient rather than the presence or absence of cancer. For all groups except healthy pregnant women, the lipid methylene composite signal linewidth correlates inversely with the fraction of mobile triglyceride present (mainly as VLDL), as estimated from resolution-enhanced spectra.
authors.affiliation: Institute of Radiology and Pathophysiology, German Cancer Research Center, Heidelberg.
authors.forename: J H
authors.initials: JH
authors.lastname: Schuhmacher
authors.forename: D
authors.initials: D
authors.lastname: Conrad
authors.forename: H G
authors.initials: HG
authors.lastname: Manke
authors.forename: J H
authors.initials: JH
authors.lastname: Clorius
authors.forename: E R
authors.initials: ER
authors.lastname: Matys
authors.forename: H
authors.initials: H
authors.lastname: Hauser
authors.forename: I
authors.initials: I
authors.lastname: Zuna
authors.forename: W
authors.initials: W
authors.lastname: Maier-Borst
authors.forename: W E
authors.initials: WE
authors.lastname: Hull
mesh_terms: D001798:Blood Proteins / Q000378:metabolism; D005260:Female; D005340:Fibrinogen / Q000378:metabolism; D006801:Humans; D008074:Lipoproteins / Q000378:metabolism; D008171:Lung Diseases / Q000097:blood / Q000175:diagnosis; D008175:Lung Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000556:secondary; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008433:Mathematics; D011247:Pregnancy
chemical_list: D001798:Blood Proteins; D008074:Lipoproteins; D005340:Fibrinogen