Dose response of N-butyl-N-(4-hydroxybutyl)nitrosamine on urinary bladder carcinogenesis in mutant ODS rats lacking L-ascorbic acid synthesizing ability.
With a daily intake of 250 ppm total ascorbic acid, ODS and F344 male rats were given 0.0125%, 0.025% or 0.05% N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN) in the drinking water for 12 weeks, and additional groups received 0.05% BBN for 2, 4 or 8 weeks. The experiment was terminated after a total of 36 weeks. A greater response to urinary bladder carcinogenesis was observed in both strains with increasing dose of BBN or longer treatment period. However, the magnitude of urinary bladder carcinogenesis in ODS rats given the higher BBN concentrations and/or long periods of BBN treatment was less than in comparably treated F344 rats, but not with lower concentrations of BBN and/or shorter periods of BBN treatment.
Mori
S
S
First Department of Pathology, Nagoya City University Medical School, Japan.
Murai
T
T
Takeuchi
Y
Y
Toyama
M
M
Makino
S
S
Konishi
T
T
Hayashi
Y
Y
Kurata
Y
Y
Fukushima
S
S
D000818:Animals; D001205:Ascorbic Acid / Q000096:biosynthesis*; D002085:Butylhydroxybutylnitrosamine / Q000633:toxicity*; D015197:Carcinogenicity Tests; D004198:Disease Susceptibility; D004305:Dose-Response Relationship, Drug; D008297:Male; D009602:Nitrosamines / Q000633:toxicity*; D009780:Occult Blood; D051381:Rats; D011916:Rats, Inbred F344; D011922:Rats, Mutant Strains; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced* / Q000473:pathology / Q000652:urine
D009602:Nitrosamines; D002085:Butylhydroxybutylnitrosamine; D001205:Ascorbic Acid