[Analysis of adhesive proteins on the surface membrane of platelet in malignant neoplasm].
Flow cytometry was used to detect fibrinogen (platelet associated fibrinogen: PAFbg) and fibronectin (PAFn) on the surface membrane of platelets in leukemia (9 cases), lung cancer (15 cases) and hepatoma (8 cases) patients (by one color analysis method), and simultaneously to investigate the binding of monoclonal anti-glycoprotein (GP) IIb/IIIa and anti-GP Ib antibodies (by two color analysis). All patient groups showed higher Fbg values than the normal control group, but no differences were found between patient groups. The values of Fbg and Fn showed a correlation, but the pattern of binding did not show a regular tendency in any patient group. There also was no significant correlation between Fbg values and the positive percentage of monoclonal anti-GPIIb/IIIa and anti-GPIb antibodies, and the binding of Fbg did not inhibit that of monoclonal antibody. The results suggest the following. 1. There are no differences in platelet activation in leukemia, lung cancer and hepatoma patients, and the degree of platelet activation is decided by the degree and the kind of stimulation. 2. The increase of both PAFbg and PAFn prove to the existence of activated platelet.
Soga
T
T
First Department of Internal Medicine, Kansai Medical University, Osaka.
Fukuroi
T
T
Yanabu
M
M
Sone
N
N
Nagata
H
H
Nomura
S
S
Kokawa
T
T
Yasunaga
K
K
D001792:Blood Platelets / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D005340:Fibrinogen / Q000378:metabolism*; D005353:Fibronectins / Q000097:blood*; D005434:Flow Cytometry; D006801:Humans; D009369:Neoplasms / Q000097:blood*; D010973:Platelet Adhesiveness
D005353:Fibronectins; D005340:Fibrinogen