Monochromosomal rodent-human hybrids from microcell fusion of human lymphoblastoid cells containing an inserted dominant selectable marker.
An improved system for the production of a series of rodent-human hybrids selectively retaining single human chromosomes marked in known locations is described. Such hybrids have significant applications in gene mapping and other genetic studies. Human lymphoblastoid lines were infected with the retroviral vector SP-1, which contains the bacterial his-D gene allowing mammalian cells to grow in the presence of histidinol. Microcell fusion of the infected lymphoblastoid cells with CHO cells was used to produce hybrids containing single human chromosomes retained by histidinol selection. Hybrids containing a single human chromosome 9 and a single human chromosome 19 are described. These have been characterized cytogenetically by G-banding, in situ hybridization, and Southern blot analysis.
Warburton
D
D
Department of Genetics and Development, Columbia University, New York 10032.
Gersen
S
S
Yu
M T
MT
Jackson
C
C
Handelin
B
B
Housman
D
D
D000818:Animals; D015139:Blotting, Southern; D002455:Cell Division / Q000187:drug effects; D002459:Cell Fusion; D002460:Cell Line; D002877:Chromosomes, Human; D006224:Cricetinae; D005819:Genetic Markers; D006641:Histidinol / Q000494:pharmacology; D006801:Humans; D006822:Hybrid Cells; D007621:Karyotyping; D008214:Lymphocytes
D005819:Genetic Markers; D006641:Histidinol