Influence of the endometrium, protease inhibitors and freezing on antiviral activity of proteins secreted by pig conceptuses.
In Exp. 1, only medium from cultures containing conceptus tissue had antiviral activity (P less than 0.05). Addition of Day-15 pregnant endometrium or Day-14 cyclic uterine flush proteins to cultures containing 200 mg conceptus tissue decreased antiviral activity (conceptus x endometrial protein interaction, P less than 0.06). Effects of endometrium (-54%) and uterine flush proteins (-40%) on antiviral activity of conceptus cultures did not differ from each other (P greater than 0.10). In Exp. 2, antiviral activity was only detected in cultures containing conceptus tissue (P less than 0.06). The amount of antiviral activity in cultures of Day-15 conceptus tissue was not influenced differently (P greater than 0.10) by culture in medium conditioned by endometrium from Day 10 or Day 12 of pregnancy. However, antiviral activity was undetectable in medium conditioned by endometrium from one of the Day-12 gilts. In Exp. 3, antiviral activity was present in medium from only 1 of 3 cultures from Day-12 gilts when assayed unfrozen. Antiviral activity was lower (P less than 0.01) in cultures of conceptuses from Day 12 than Day 14 of pregnancy; however, antiviral activity increased quadratically (P less than 0.05) when cultures contained 0, 0.01, 0.1 and 1.0 units/ml aprotinin, respectively. Freezing and thawing culture medium did not reduce (P greater than 0.10) antiviral activity compared to medium assayed unfrozen (1438 vs 1354 units/ml, respectively). These results suggest a regulatory influence of the endometrium on secretion of antiviral proteins by pig conceptuses in vitro.
Beers
S
S
Department of Animal Science, University of Florida, Gainesville 32611.
Mirando
M A
MA
Pontzer
C H
CH
Harney
J P
JP
Torres
B A
BA
Johnson
H M
HM
Bazer
F W
FW
D000818:Animals; D000998:Antiviral Agents / Q000378:metabolism*; D004717:Endometrium / Q000502:physiology*; D005260:Female; D005615:Freezing; D011247:Pregnancy; D011480:Protease Inhibitors / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism*; D013552:Swine / Q000196:embryology* / Q000378:metabolism
D000998:Antiviral Agents; D011480:Protease Inhibitors; D011506:Proteins