A specific, sensitive radioimmunoassay for platelet-activating factor (PAF).
A specific radioimmunoassay (RIA) has been developed for platelet-activating factor (PAF) and shown to be sensitive over the range 10-1000 pg (0.02-2 pmol). The anti-PAF antibodies showed specificity for the acetyl group at the C2 position of the PAF molecule and exhibited no significant cross-reactivity with lyso-PAF or the naturally occurring lipids including lecithin and lysolecithin. The sensitivity of the RIA was at least as good as the platelet-based assays for PAF but the RIA was simpler to perform, had a higher capacity and did not have the drawback of the inherent variability associated with the bioassays.
Smal
M A
MA
Kolling Institute of Medical Research, Royal North Shore Hospital of Sydney, NSW, Australia.
Baldo
B A
BA
McCaskill
A
A
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D006801:Humans; D008055:Lipids / Q000276:immunology; D010972:Platelet Activating Factor / Q000032:analysis* / Q000276:immunology; D011863:Radioimmunoassay; D015203:Reproducibility of Results; D012463:Saliva / Q000032:analysis; D012756:Sheep
D000906:Antibodies; D008055:Lipids; D010972:Platelet Activating Factor