Radioimmunoassay of aescine, a mixture of triterpene glycosides.
A radioimmunoassay (RIA) has been developed for the determination of picogram amounts of aescine, an anti-inflammatory and anti-oedematous glycoside mixture from unpurified extracts of Aesculus hippocastanum. Practically no interference is observed for various potentially crossreacting compounds. This RIA covers the range 100 pg-50 ng, within which acceptable accuracy and precision are obtained.
Lehtola
T
T
Department of Pharmaceutical Chemistry, University of Kuopio, Finland.
Huhtikangas
A
A
D002240:Carbohydrate Sequence; D011232:Chemical Precipitation; D003429:Cross Reactions; D003432:Cross-Linking Reagents; D004928:Escin / Q000032:analysis*; D005022:Ethyldimethylaminopropyl Carbodiimide; D007106:Immune Sera; D008969:Molecular Sequence Data; D010944:Plants / Q000032:analysis*; D011092:Polyethylene Glycols; D011863:Radioimmunoassay; D012503:Saponins / Q000032:analysis*; D012639:Seeds / Q000032:analysis; D012710:Serum Albumin, Bovine
D003432:Cross-Linking Reagents; D007106:Immune Sera; D012503:Saponins; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D004928:Escin; D005022:Ethyldimethylaminopropyl Carbodiimide