Nucleation of macrotubules on double-walled microtubule seeds.
It is known that histone H1 is able to cause the formation of double-walled microtubules from microtubule protein. Now, we demonstrate that in dependence on the mass ratio H1/microtubule protein upon addition of tubulin to short pieces of double-walled microtubules either their inner or their outer wall elongates resulting in normal microtubules or in macrotubules, respectively. Because of their genesis we suggest that macrotubules like double-walled microtubules (see Unger et al., Eur. J. Cell Biol. 46, 98-104 (1988)) expose those sides of tubulin dimers at their surface which usually form the lumen face of microtubules.
Böhm
K J
KJ
Central Institute of Microbiology and Experimental Therapy, Academy of Sciences of the GDR, Jena.
Vater
W
W
Steinmetzer
P
P
Unger
E
E
D000818:Animals; D006657:Histones / Q000032:analysis*; D008854:Microscopy, Electron; D008868:Microtubule Proteins / Q000032:analysis*; D008870:Microtubules / Q000032:analysis / Q000648:ultrastructure*; D014404:Tubulin / Q000494:pharmacology
D006657:Histones; D008868:Microtubule Proteins; D014404:Tubulin