title: Purification and characterization of a novel phospholipid transfer protein from filamentous fungi.
abstract: 1. We have isolated from mycelia of Mucor mucedo, a filamentous fungus, a phospholipid transfer protein. 2. The purification steps were gel filtration, hydroxyapatite chromatography, blue affinity column and fast protein liquid chromatography on anion exchanger. 3. A purified protein was obtained with a molecular mass of 24 kDa and a pI of 5.05 and its N-terminal sequence was established. 4. This protein transfers phosphatidylinositol, as well as phosphatidylcholine and phosphatidylethanolamine.
authors.affiliation: Laboratoire de Cryptogamie, Université Paul Sabatier, Toulouse, France.
authors.forename: P
authors.initials: P
authors.lastname: Grondin
authors.forename: C
authors.initials: C
authors.lastname: Vergnolle
authors.forename: L
authors.initials: L
authors.lastname: Chavant
authors.forename: J C
authors.initials: JC
authors.lastname: Kader
mesh_terms: D000595:Amino Acid Sequence; D000645:Ammonium Sulfate; D002352:Carrier Proteins / Q000302:isolation & purification*; D011232:Chemical Precipitation; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D008565:Membrane Proteins / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009089:Mucor / Q000032:analysis* / Q000254:growth & development; D049294:Phospholipid Transfer Proteins; D010743:Phospholipids / Q000302:isolation & purification*
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; D049294:Phospholipid Transfer Proteins; D010743:Phospholipids; D000645:Ammonium Sulfate