Assessment of protein reorientational diffusion in solution by 13C off-resonance rotating frame spin-lattice relaxation: effect of polydispersity.
The 13C off-resonance rotating frame spin-lattice relaxation technique is applicable to the study of protein rotational diffusion behavior in both model in vitro and in vivo systems. The original formalism of James and co-workers [(1978) J. Am. Chem. Soc. 100, 3590-3594] was constrained by the assumption of random isotropic reorientational motion of a monodisperse protein population. Here we extend the formalism to include polydispersity. Application is made to the alkaline pH induced association of lysozyme, lysozyme-bovine serum albumin mixtures, and to the phase separation of lysozyme salt-water mixtures induced by low temperature.
Morgan
C F
CF
Department of Chemistry, University of California, Santa Cruz 95064.
Schleich
T
T
Caines
G H
GH
D002247:Carbon Isotopes; D004058:Diffusion; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008954:Models, Biological; D009113:Muramidase; D011487:Protein Conformation; D011506:Proteins; D012710:Serum Albumin, Bovine; D012996:Solutions
D002247:Carbon Isotopes; D011506:Proteins; D012996:Solutions; D012710:Serum Albumin, Bovine; D009113:Muramidase