Presence of HBV-DNA in peripheral blood mononuclear cells from anti-HIV symptomless carriers.
The presence of hepatitis B virus DNA (HBV-DNA) in the peripheral blood mononuclear cells (PBMC) of 29 anti-HIV symptomless carriers (eleven HBeAg positive, eleven anti-HBe positive and seven HBsAg negative) and of 40 anti-human immunodeficiency virus (HIV)-negative patients (15 HBeAg positive, 15 anti-HBe positive and ten HBsAg negative) has been studied by dot-blot and Southern blot hybridization. HBV-DNA has been found in similar proportions in both anti-HIV-positive and negative patients (36% and 46%, respectively, in the HBeAg positive group and 27% and 37% in the anti-HBe positive group). No HBV-DNA was detected in the PBMC of the HBsAg-negative patients. No relation has been observed between the presence of HBV-DNA in the PBMC of the anti-HIV-positive patients and the detection of HIV antigen (HIV Ag), number of CD4 cells or the CD4/CD8 ratio. In summary, the presence of HBV-DNA in the PBMC of anti-HIV symptomless carriers does not seem to imply that the patient's clinical state has worsened.
Bartolomé
F J
FJ
Department of Gastroenterology, Fundación Jimenez Diaz, Universidad Autónoma, Madrid, Spain.
Moraleda
G
G
Castillo
I
I
Martinez
M G
MG
Porres
J C
JC
Carreño
V
V
D000410:Alanine Transaminase / Q000097:blood; D015139:Blotting, Southern; D002353:Carrier State / Q000382:microbiology*; D004279:DNA, Viral / Q000097:blood*; D006679:HIV Seropositivity / Q000382:microbiology*; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D009000:Monocytes / Q000382:microbiology*
D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D000410:Alanine Transaminase