Rapid diagnosis of typhoid fever through identification of Salmonella typhi within 18 hours of specimen acquisition by culture of the mononuclear cell-platelet fraction of blood.
Detection of Salmonella typhi in blood by culture of the mononuclear cell-platelet layer was compared with other methods currently used for the diagnosis of typhoid fever. Colonies of S. typhi were present in all mononuclear cell-platelet layer-positive cultures within 18 h of plating and were identified within an additional 10 min by a coagglutination technique. In contrast, identification of all positive cultures by conventional blood culture required 3 days.
Rubin
F A
FA
Department of Bacterial Immunology, Walter Reed Army Institute of Research, Washington, D.C. 20307-5100.
McWhirter
P D
PD
Burr
D
D
Punjabi
N H
NH
Lane
E
E
Kumala
S
S
Sudarmono
P
P
Pulungsih
S P
SP
Lesmana
M
M
Tjaniadi
P
P
D000372:Agglutination Tests; D001792:Blood Platelets / Q000382:microbiology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000382:microbiology*; D012485:Salmonella typhi / Q000302:isolation & purification*; D013048:Specimen Handling; D013997:Time Factors; D014435:Typhoid Fever / Q000175:diagnosis*