Effects of antiarrhythmic agents classified as class III group on ischaemia-induced myocardial damage in canine hearts.
1. The cardioprotective effects of antiarrhythmic agents classified as class III, amiodarone, sotalol and E-4031, were investigated in anaesthetized dogs. 2. The left anterior descending coronary artery was occluded for 2 h. 3. Heart mitochondria were prepared from both the ischaemic and non-ischaemic areas, and their function was estimated polarographically. 4. Activities of the lysosomal enzymes, N-acetyl-beta-glucosaminidase and beta-glucuronidase, were measured in each fraction. 5. Two hour occlusion induced ventricular arrhythmias, and amiodarone, sotalol and E-4031 greatly suppressed the development of arrhythmias. 6. Amiodarone, sotalol and E-4031 significantly protected mitochondria against ischaemia, and prevented ischaemia-induced leakage of lysosomal enzymes. 7. Antiarrhythmic agents classified as class III show cardioprotective effects, which might participate in their antiarrhythmic effect.
Sano
T
T
Department of Anesthesiology, Faculty of Medicine, University of Nagoya, Japan.
Sugiyama
S
S
Taki
K
K
Hanaki
Y
Y
Shimada
Y
Y
Ozawa
T
T
D000118:Acetylglucosaminidase / Q000378:metabolism; D000638:Amiodarone / Q000494:pharmacology; D000758:Anesthesia; D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects; D003327:Coronary Disease / Q000201:enzymology / Q000503:physiopathology*; D003331:Coronary Vessels / Q000187:drug effects; D004285:Dogs; D004562:Electrocardiography; D005260:Female; D006339:Heart Rate / Q000187:drug effects; D066298:In Vitro Techniques; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects; D010880:Piperidines / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D013015:Sotalol / Q000494:pharmacology
D000889:Anti-Arrhythmia Agents; D010880:Piperidines; D011725:Pyridines; C063968:E 4031; D013015:Sotalol; D000118:Acetylglucosaminidase; D000638:Amiodarone