Genetic identification of non-specific esterases of the mouse cauda epididymidis and description of esterase-28, a new carboxylesterase isoenzyme (EC 3.1.1.1).
Twenty-five (25) electrophoretic bands with esterase activity were distinguished in supernatants of cauda epididymidis of DBA/2J mice. Twenty (20) of these were assigned to 10 genetically defined esterases (ES-1, ES-2, ES-3, ES-6, ES-7, ES-11, ES-14, ES-17, ES-19, ES-22) which were already known from investigations of other mouse tissues. Furthermore, ES-10 was identified in cauda supernatants after isoelectric focussing. A hitherto genetically undefined esterase was assigned to locus Es-28 which was expressed solely in the epididymis. Three phenotypes were distinguished: ES-28A was present in the majority of the inbred strains examined. ES-28B was observed in AKR/Han mice and ES-28C was found in SEG/1 mice.
von Deimling
O
O
Pathologisches Institut, Abteilung für Chemische Pathologie, Universität Freiburg, FRG.
Wassmer
B
B
D000483:Alleles; D000818:Animals; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000032:analysis / Q000235:genetics*; D004589:Electrophoresis, Disc; D004822:Epididymis / Q000201:enzymology*; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000235:genetics*; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D010641:Phenotype
D007527:Isoenzymes; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase