title: Age-related changes of the ciliary muscle in comparison with changes induced by treatment with prostaglandin F2 alpha. An ultrastructural study in rhesus and cynomolgus monkeys.
abstract: The relationship between individual ciliary muscle cells and the surrounding connective tissue was studied in the eyes of three normal, young (3-4 years) cynomolgus monkeys (Macaca fascicularis), three aged (34-36 years) rhesus monkeys (Macaca mulatta) and seven young (3-7 years) cynomolgus monkeys topically treated with prostaglandin F2 alpha (PGF2 alpha) for 4-8 days. In normal eyes, collagen fibrils and microfibrils are in places in continuity with the muscle cells' basal lamina, which is connected to the cell membrane by fine fibrillous material. In old eyes, the basal lamina is markedly thickened, masking the connection of fibrils with the muscle cells' membrane. A distinctive finding in several muscle cells of old eyes are electronlucent clefts, 60-80 nm wide, between basal lamina and cell membrane, which are not transversed by fibrils or fibrillous material. The cell membrane of these muscle cells shows large folds filled with disarranged myofilaments. Additionally, these cells contain inclusion bodies consisting of concentrically arranged double membranes. Following treatment with PGF2 alpha, similar changes are seen in young animals, too. Here, the muscle cells have lost their connection to the extracellular fibrils due to a PGF2 alpha-induced lysis of extracellular material. Lack of attachment between basal lamina and altered muscle cells in aged eyes might indicate an involvement of the extracellular matrix in age-related changes of the individual ciliary muscle cells.
authors.affiliation: University of Erlangen-Nürnberg, Department of Anatomy, F.R.G.
authors.forename: E
authors.initials: E
authors.lastname: Tamm
authors.forename: E
authors.initials: E
authors.lastname: Lütjen-Drecoll
authors.forename: J W
authors.initials: JW
authors.lastname: Rohen
mesh_terms: D000375:Aging / Q000473:pathology*; D000818:Animals; D001485:Basement Membrane / Q000187:drug effects / Q000503:physiopathology / Q000648:ultrastructure*; D066298:In Vitro Techniques; D008252:Macaca fascicularis; D008253:Macaca mulatta; D009801:Oculomotor Muscles / Q000187:drug effects / Q000503:physiopathology / Q000648:ultrastructure*; D011460:Prostaglandins F / Q000494:pharmacology*
chemical_list: D011460:Prostaglandins F