Pig lung 5'-nucleotidase: effect of diadenosine 5',5'''-P1, P4-tetraphosphate and its related compounds.
1. A 5'-nucleotidase was purified from pig lung to apparent homogeneity. 2. Its kinetic properties were similar to those of the previously reported cytoplasmic 5'-nucleotidase, which preferentially hydrolyses IMP and GMP. 3. It was a tetramer composed of 69 kDa subunit. 4. It was effectively stimulated by diadenosine tetraphosphate and glycerate 2,3-bisphosphate.
Itoh
R
R
National Institute of Nutrition, Tokyo, Japan.
Yamada
K
K
D015720:5'-Nucleotidase / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D002850:Chromatography, Gel; D002853:Chromatography, Liquid; D015226:Dinucleoside Phosphates / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008168:Lung / Q000201:enzymology*; D008970:Molecular Weight; D013552:Swine
D015226:Dinucleoside Phosphates; C020733:diadenosine tetraphosphate; D015720:5'-Nucleotidase