title: Isolation and structural analysis of three new disialylated oligosaccharides from human milk.
abstract: Five disialylated oligosaccharides, of which three were new compounds, were isolated from pooled human milk. The isolation procedure included gel filtration and ion-exchange chromatography and the final purification was accomplished by HPLC using a mobile phase containing triethylamine as an ion-pairing reagent. Structural analysis was carried out by chemical analyses, fast atom bombardment mass spectrometry, and 500-MHz NMR spectroscopy. The following new structures were found: (formula; see text).
authors.affiliation: Department of Carbohydrate Chemistry, University of Lund, Sweden.
authors.forename: G
authors.initials: G
authors.lastname: Grönberg
authors.forename: P
authors.initials: P
authors.lastname: Lipniunas
authors.forename: T
authors.initials: T
authors.lastname: Lundgren
authors.forename: F
authors.initials: F
authors.lastname: Lindh
authors.forename: B
authors.initials: B
authors.lastname: Nilsson
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008895:Milk, Human / Q000032:analysis*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000302:isolation & purification*; D012459:Salicylates / Q000302:isolation & purification*
chemical_list: D009844:Oligosaccharides; D012459:Salicylates; C014182:salicylsalicylic acid