Arrangement of cytoskeletal filaments at the equator of chicken intrafusal muscle fibers.
The organization of the cytoskeleton at the equator of chicken intrafusal fibers was examined with immunofluorescence light microscopy, using monoclonal antibodies against myosin heavy chains, desmin, actin and tropomyosin. Actin was localized in the cytosol and in equatorial nuclei, while myosin heavy chains, desmin and tropomyosin were only observed in the cytosol. Although all four proteins were present at the equator and at the pole, the fluorescence produced after incubation with the different antibodies varied considerably between the two regions. Staining at the pole was in the form of striations, but at the equator it was non-striated and more uniform. The observed fluorescent patterns suggest that at the equator filaments are assembled into looser arrays than in the sarcomeres of the pole. A flexible cytoskeleton at the equator would be an appropriate substrate for distorting the affixed sensory endings during an applied stress.
Maier
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A
Department of Cell Biology and Anatomy, University of Alabama, Birmingham 35294.
Zak
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R
D000199:Actins / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002645:Chickens / Q000033:anatomy & histology*; D003599:Cytoskeleton / Q000032:analysis*; D003893:Desmin / Q000276:immunology; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009132:Muscles / Q000648:ultrastructure*; D009218:Myosins / Q000276:immunology; D014335:Tropomyosin / Q000276:immunology
D000199:Actins; D000911:Antibodies, Monoclonal; D003893:Desmin; D014335:Tropomyosin; D009218:Myosins