title: Structure and orientation of expressed bovine coronavirus hemagglutinin-esterase protein.
abstract: The sequence of the hemagglutinin-esterase (HE) gene for the Mebus strain of bovine coronavirus was obtained from cDNA clones, and its deduced product is a 47,700-kilodalton apoprotein of 424 amino acids. Expression of the HE protein in vitro in the presence of microsomes revealed N-terminal signal peptide cleavage and C-terminal anchorage but not disulfide-linked dimerization. Dimerization was observed only after expression in vivo, during which HE was also transported to the cell surface.
authors.affiliation: Department of Microbiology, University of Tennessee, Knoxville 37996-0845.
authors.forename: T E
authors.initials: TE
authors.lastname: Kienzle
authors.forename: S
authors.initials: S
authors.lastname: Abraham
authors.forename: B G
authors.initials: BG
authors.lastname: Hogue
authors.forename: D A
authors.initials: DA
authors.lastname: Brian
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001692:Biological Transport; D002417:Cattle; D003332:Coronaviridae / Q000201:enzymology / Q000235:genetics*; D015870:Gene Expression; D005814:Genes, Viral; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D006389:Hemagglutinins, Viral / Q000235:genetics* / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism; D008969:Molecular Sequence Data; D011499:Protein Processing, Post-Translational / Q000235:genetics; D014760:Viral Fusion Proteins; D014764:Viral Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D006023:Glycoproteins; D006389:Hemagglutinins, Viral; D014760:Viral Fusion Proteins; D014764:Viral Proteins; C060814:hemagglutinin esterase