Fruit allergy: demonstration of IgE antibodies to a 30 kd protein present in several fruits.
A patient who had experienced allergic responses to various fruits developed an acute anaphylactic reaction after the ingestion of a local strain of cling peaches. The patient's serum, but not control sera, contained IgE antibodies reactive to extracts from peaches, guavas, bananas, mandarins, and strawberries in an ELISA. The patient's serum, however, did not demonstrate elevated levels of IgE antibodies to extracts from apples, pears, and nectarines. Adsorption of the patient's serum with extracts from peaches, strawberries, and mandarins resulted in a decline of detectable IgE antibodies to these fruits. Adsorption with extracts from apples and pears had no such effect. These results demonstrate the specificity of the patient's IgE antibodies to selected fruits only. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by Western blotting revealed a 30 kda of protein that was recognized by the IgE antibodies in the patient's serum. This protein was not present in extracts from pears or apples. Our results highlight the importance of specific allergens in considering acute allergic reactions to individuals exhibiting sensitivity to various foods or fruits.
Wadee
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AA
Department of Immunology, School of Pathology, South African Institute for Medical Research, Johannesburg.
Boting
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LA
Rabson
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AR
D000328:Adult; D000485:Allergens / Q000276:immunology*; D000707:Anaphylaxis / Q000097:blood / Q000209:etiology / Q000276:immunology; D000918:Antibody Specificity; D015153:Blotting, Western; D005260:Female; D005512:Food Hypersensitivity / Q000097:blood / Q000276:immunology*; D005638:Fruit / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis*; D008970:Molecular Weight; D010936:Plant Extracts / Q000032:analysis; D010940:Plant Proteins / Q000276:immunology*
D000485:Allergens; D010936:Plant Extracts; D010940:Plant Proteins; D007073:Immunoglobulin E