title: A serine protease triad forms the catalytic centre of a triacylglycerol lipase.
abstract: True lipases attach triacylglycerols and act at an oil-water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 A resolution and refined to 1.9 A resolution. It reveals a Ser..His..Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.
authors.affiliation: Department of Chemistry, University of York, Heslington, UK.
authors.forename: L
authors.initials: L
authors.lastname: Brady
authors.forename: A M
authors.initials: AM
authors.lastname: Brzozowski
authors.forename: Z S
authors.initials: ZS
authors.lastname: Derewenda
authors.forename: E
authors.initials: E
authors.lastname: Dodson
authors.forename: G
authors.initials: G
authors.lastname: Dodson
authors.forename: S
authors.initials: S
authors.lastname: Tolley
authors.forename: J P
authors.initials: JP
authors.lastname: Turkenburg
authors.forename: L
authors.initials: L
authors.lastname: Christiansen
authors.forename: B
authors.initials: B
authors.lastname: Huge-Jensen
authors.forename: L
authors.initials: L
authors.lastname: Norskov
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D004220:Disulfides; D008049:Lipase; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009089:Mucor / Q000201:enzymology*; D010446:Peptide Fragments; D011487:Protein Conformation; D012697:Serine Endopeptidases; D014961:X-Ray Diffraction
chemical_list: D004220:Disulfides; D010446:Peptide Fragments; D008049:Lipase; D012697:Serine Endopeptidases