Immunomagnetic method to select human sperm without sperm surface-bound autoantibodies in male autoimmune infertility.
Using supermagnetic polymer microspheres coated with anti-immunoglobulins, spermatozoa without autoantibodies bound on their surface can be isolated from a sperm population showing a variable percentage of cells with autoantibodies bound on their surface. This simple technique seems to induce no modification of semen qualities; therefore, the concentration of sperm after immunomagnetic separation might be useful for in vivo or in vitro insemination in infertile couples with autoimmune male infertility.
Foresta
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C
Institute of Semeiotica Medica, University of Padua, Italy.
Varotto
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Caretto
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A
D001323:Autoantibodies / Q000032:analysis*; D001327:Autoimmune Diseases / Q000276:immunology*; D002469:Cell Separation / Q000379:methods*; D006801:Humans; D007248:Infertility, Male / Q000276:immunology*; D008280:Magnetics; D008297:Male; D013094:Spermatozoa / Q000032:analysis / Q000276:immunology*
D001323:Autoantibodies