title: A new sensitive microassay for the measurement of erythrocyte glycogen.
abstract: Conventional ethanol precipitation of sub-microgram amounts of glycogen leads to low yields (less than 50%). Quantitative recoveries of 90% were attained, however, when the isolation temperature was raised to 50 degrees C and ethanol was replaced by the less polar propan-2-ol. This improvement enabled development of an erythrocyte assay for glycogen which was both sensitive (0.1 microgram glycogen) and required only 1 ml of whole blood. 26 paediatric specimens were analysed and a reference range of values from undetected to 78 micrograms glycogen/g haemoglobin (Hb) was obtained.
authors.affiliation: Department of Biochemistry, Royal Hospital for Sick Children, Glasgow, Scotland, UK.
authors.forename: J
authors.initials: J
authors.lastname: Farquharson
authors.forename: E C
authors.initials: EC
authors.lastname: Jamieson
authors.forename: G B
authors.initials: GB
authors.lastname: MacPhee
authors.forename: R W
authors.initials: RW
authors.lastname: Logan
mesh_terms: D000433:1-Propanol; D004912:Erythrocytes / Q000032:analysis*; D006003:Glycogen / Q000097:blood*; D006358:Hot Temperature; D006801:Humans; D008832:Microchemistry
chemical_list: D006003:Glycogen; D000433:1-Propanol