Preferential uptake of benzoporphyrin derivative by leukemic versus normal cells.
Benzoporphyrin derivatives (BPDs) are photosensitizers, which fluoresce strongly at 690 nm, and may be candidates for various applications of photodynamic therapy (PDT). Fluorescence-activated cell sorting (FACS) analysis, subsequent to ultraviolet light excitation, revealed pronounced differences in red fluorescence between leukemic cell lines (HL60, K562 and L1210), leukemic clinical isolates, and normal human or murine bone marrow cells incubated with BPD. These observed differences in BPD-mediated fluorescence provide the rationale for sorting leukemic from normal cells via FACS or may constitute a novel method for extracorporeal purging of remission marrow by photodynamic therapy in autologous bone marrow transplantation.
Jamieson
C H
CH
Department of Microbiology, University of British Columbia, Vancouver, Canada.
McDonald
W N
WN
Levy
J G
JG
D000818:Animals; D001692:Biological Transport; D001853:Bone Marrow / Q000378:metabolism; D002460:Cell Line; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D007938:Leukemia / Q000097:blood / Q000378:metabolism*; D007939:Leukemia L1210 / Q000378:metabolism; D051379:Mice; D008811:Mice, Inbred DBA; D009000:Monocytes / Q000378:metabolism*; D011166:Porphyrins / Q000378:metabolism*; D011838:Radiation-Sensitizing Agents / Q000378:metabolism*; D012016:Reference Values; D013154:Spleen / Q000378:metabolism; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured / Q000378:metabolism*
D011166:Porphyrins; D011838:Radiation-Sensitizing Agents; C504224:benzoporphyrin D