Rapid, robust method for measuring low concentrations of albumin in urine.
We describe a rapid particle-enhanced turbidimetric immunoassay for albumin in urine. Intra- and interassay CVs were less than 5% and less than 10%, respectively, the detection limit is 2 mg/L, and the working range extends to 200 mg/L. Mean analytical recovery of albumin added to centrifuged urines was 100% (SD 10.6%), and, when results were compared with those by the Pharmacia RIA, the correlation coefficient was 0.99. The working reagents are stable for at least six months; thus this assay is suited for both batch and urgent analysis.
Medcalf
E A
EA
Department of Clinical Biochemistry, London Hospital Medical College, U.K.
Newman
D J
DJ
Gorman
E G
EG
Price
C P
CP
D000419:Albuminuria / Q000652:urine*; D006801:Humans; D007118:Immunoassay / Q000706:statistics & numerical data; D009391:Nephelometry and Turbidimetry; D011786:Quality Control; D011863:Radioimmunoassay