Rapid microwave-stimulated fixation of fine-needle aspiration biopsies for transmission electron microscopy.
A new technique is described in which fine-needle aspiration biopsies are processed for transmission electron microscopy within 2 hr. This rapid processing method includes exposure of the biopsy in a glutaraldehyde-formaldehyde mixture to microwaves for 25 sec in a domestic oven. The specimen is then routinely stained, processed through graded ethanol, and embedded in epoxy resin to produce excellent preservation of fine structural features.
Gove
D W
DW
Electron Microscopy Unit, Queen Elizabeth Hospital Woodville, South Australia.
Lang
C A
CA
Waterhouse
L K
LK
Leong
A S
AS
D001707:Biopsy, Needle / Q000379:methods*; D002277:Carcinoma / Q000473:pathology*; D005404:Fixatives; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D008854:Microscopy, Electron / Q000379:methods*; D008872:Microwaves
D005404:Fixatives