Iontophoretic injection of fluoro-gold and other fluorescent tracers.
Fluoro-gold, a retrograde axonally transported fluorescent tracer, has been successfully iontophoresed into brain regions to yield small injection sites with no detectable dye along the track. Previous difficulties involving iontophoretic application of fluoro-gold were overcome through the use of a cacodylate vehicle. This vehicle was also found to be suitable for iontophoresing other fluorescent tracers, such as propidium iodide or Fast Blue.
Schmued
L C
LC
Department of Otolaryngology, University of Virginia Medical Center, Charlottesville 22908.
Heimer
L
L
D000818:Animals; D001921:Brain / Q000378:metabolism; D002021:Buffers; D002101:Cacodylic Acid; D005456:Fluorescent Dyes / Q000008:administration & dosage*; D007478:Iontophoresis; D008297:Male; D008856:Microscopy, Fluorescence; D051381:Rats; D013266:Stilbamidines
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D002021:Buffers; D005456:Fluorescent Dyes; D013266:Stilbamidines; D002101:Cacodylic Acid