Suppression of transferrin internalization in myogenic L6 cells by dibucaine.
Dibucaine, a potent local anesthetic, is known to suppress myogenesis. The promotion of myogenesis requires transferrin (Tf) which transports Fe to the cells. Therefore, the effects of dibucaine on Fe uptake and Tf internalization were studied using myogenic cell line L6. Dibucaine at 200 microM suppressed 55Fe accumulation which was transported by 55Fe-transferrin to the cells. The anesthetic changed neither the number of Tf receptors nor the affinity of Tf to Tf receptors on the cell membrane. Dibucaine retarded the endocytosis and exocytosis cycle of Tf, and this retardation acted to suppress Fe accumulation.
Hagiwara
Y
Y
Division of Cell Biology, National Institute of Neuroscience, Tokyo, Japan.
Ozawa
E
E
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects; D003992:Dibucaine / Q000494:pharmacology*; D007501:Iron / Q000378:metabolism; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011990:Receptors, Transferrin / Q000378:metabolism; D014168:Transferrin / Q000378:metabolism*
D011990:Receptors, Transferrin; D014168:Transferrin; D007501:Iron; D003992:Dibucaine