Electroporation as a new technique for producing transgenic fish.
A recombinant plasmid, pMV-GH, containing rainbow trout growth hormone cDNA fused to mouse metallothionein I promoter, was introduced into medaka (Oryzias latipes) by electroporation. Of 3109 fertilized eggs treated with electric pulses (750 V/cm, 50 microseconds, 5 times), 783 (25%) hatched out. Four percent of the hatchlings were transgenic. To obtain transgenic lines, 180 hatchlings were maintained and 35 of them grew into adult fish. Two of these fish were transgenic. When one transgenic fish was mated with a normal female, the transgene was found in all the F1 offspring assayed. In F2 offspring obtained by mating transgenic F1 fish, 88% were transgenic.
Inoue
K
K
Central Research Laboratory, Nippon Suisan Kaisha, Tokyo, Japan.
Yamashita
S
S
Hata
J
J
Kabeno
S
S
Asada
S
S
Nagahisa
E
E
Fujita
T
T
D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D003532:Cyprinodontiformes / Q000235:genetics*; D004247:DNA / Q000235:genetics; D004560:Electricity; D005818:Genetic Engineering / Q000379:methods*; D006133:Growth Substances / Q000235:genetics; D008668:Metallothionein / Q000235:genetics; D008722:Methods; D008845:Microinjections; D009990:Oryzias / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014162:Transfection / Q000235:genetics
D006133:Growth Substances; D004247:DNA; D008668:Metallothionein