title: Mass spectrometric confirmation of the presence of N-nitrosopyrrolidine in instant coffee.
abstract: Traces of N-nitrosopyrrolidine (NPYR) may occur in some samples of both instant coffee and fine-ground roasted coffee. The identity of NPYR in 2 samples of instant coffee was confirmed by mass spectrometry as well as by liquid chromatography-thermal energy analysis. A 2-step cleanup procedure, involving fractionation on basic alumina followed by gradient elution on reverse-phase C18 cartridge, is described that allows full-scan mass spectrometric confirmation of NPYR in tested samples.
authors.affiliation: Food Research Division, Bureau of Chemical Safety, Ottawa, Ontario, Canada.
authors.forename: N P
authors.initials: NP
authors.lastname: Sen
authors.forename: S W
authors.initials: SW
authors.lastname: Seaman
authors.forename: D
authors.initials: D
authors.lastname: Weber
mesh_terms: D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D003069:Coffee / Q000032:analysis*; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D009242:N-Nitrosopyrrolidine / Q000032:analysis*; D009602:Nitrosamines / Q000032:analysis*; D012997:Solvents
chemical_list: D003069:Coffee; D007202:Indicators and Reagents; D009602:Nitrosamines; D012997:Solvents; D009242:N-Nitrosopyrrolidine