title: Comparison of nitrogen-15 and diaminopimelic acid for estimating bacterial protein synthesis of lactating cows fed diets of varying protein degradability.
abstract: Three lactating Holstein cows fitted with duodenal cannulae were fed diets containing cottonseed meal, corn gluten meal, or blood meal as protein supplements in a 3 c 3 Latin square experiment. Diets averaged 15% CP and were 60% concentrate, 31% corn silage, and 9% alfalfa hay. The flow marker was Cr2O3; the bacterial protein fraction of digesta CP was estimated by 15N (as ammonium sulfate) and diaminopimelic acid. The undegraded fraction of total feed protein entering the duodenum for respective diets was .52, .57, and .69. The 15N method was less variable than diaminopimelic acid. Based on 15N, percentage of bacterial of total protein differed among treatments (61.5, 59.4, and 55.0, respectively). Ten percent more protein entered the duodenum on blood meal than other diets, but differences were not significant. Protein sources were similar in microbial passage, but degraded protein was used most efficiently for microbial synthesis on blood meal. Incorporation of 15N consumed into bacterial protein ranged from 50 to 83% with numerically highest values on blood meal, suggesting greater efficiency of ammonia, capture. Recoveries of 15N for the 72 h as milk, feces and urine ranged from 54 to 78%.
authors.affiliation: Department of Animal Sciences, University of Arizona, Tucson 85721.
authors.forename: M S
authors.initials: MS
authors.lastname: Sadik
authors.forename: J T
authors.initials: JT
authors.lastname: Huber
authors.forename: K
authors.initials: K
authors.lastname: King
authors.forename: R
authors.initials: R
authors.lastname: Wanderley
authors.forename: D
authors.initials: D
authors.lastname: DeYoung
authors.forename: A
authors.initials: A
authors.lastname: al-Dehneh
authors.forename: C
authors.initials: C
authors.lastname: Dudas
mesh_terms: D000821:Animal Feed; D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis*; D002417:Cattle / Q000382:microbiology* / Q000502:physiology; D003960:Diaminopimelic Acid / Q000032:analysis; D004044:Dietary Proteins / Q000378:metabolism*; D004063:Digestion; D005260:Female; D007774:Lactation; D009587:Nitrogen Isotopes
chemical_list: D001426:Bacterial Proteins; D004044:Dietary Proteins; D009587:Nitrogen Isotopes; D003960:Diaminopimelic Acid