title: Spider silkglands contain a tissue-specific alanine tRNA that accumulates in vitro in response to the stimulus for silk protein synthesis.
abstract: The large ampullate glands of the orb-web spider, Nephila clavipes provide massive amounts of fibroin throughout the lifetime of the adult female. We have developed methods to culture the glands and manipulate their biosynthetic activity. This has allowed us to monitor a series of molecular events that precede silk production in glands excised from appropriately stimulated animals. In this paper, we demonstrate that prior to the transient dramatic production of fibroin, such glands accumulate large amounts of tRNAs cognate to the abundant amino acids in spider silk. One of these, alanine tRNA, appears to consist of two isoaccepting forms--one constitutive, and the other silkgland specific. Moreover, the silkgland-specific form appears to accumulate preferentially in response to stimulation. This phenomenon of tissue-specific tRNA production appears similar to that found in the silkglands of Bombyx mori, but the spider system has the unique property of permitting manipulation in vitro. Thus, it provides an unusual opportunity to study the mechanism of regulated tRNA synthesis.
authors.affiliation: Department of Biology, University of Puerto Rico, Rio Piedras 00931.
authors.forename: G C
authors.initials: GC
authors.lastname: Candelas
authors.forename: G
authors.initials: G
authors.lastname: Arroyo
authors.forename: C
authors.initials: C
authors.lastname: Carrasco
authors.forename: R
authors.initials: R
authors.lastname: Dompenciel
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D005349:Fibroins / Q000096:biosynthesis; D009924:Organ Culture Techniques; D012344:RNA, Transfer, Ala / Q000096:biosynthesis*; D012345:RNA, Transfer, Amino Acid-Specific / Q000096:biosynthesis*; D013112:Spiders / Q000502:physiology*
chemical_list: D012344:RNA, Transfer, Ala; D012345:RNA, Transfer, Amino Acid-Specific; D005349:Fibroins