Combined use of oligo(dt) and 28S cDNA probes for the quantitation of total mRNA in polyribosomes: application to the castration-induced atrophy of the rat prostate.
The castration-induced atrophy of the rat prostate was used as a model for the validation of a sensitive technique allowing the quantitation of total mRNA in polyribosomes. Electron micrographs of polyribosome samples showed a decrease in polyribosomes length 7 days after castration (GDX). Specificity of labeled oligo(dt) probe for poly(A) was demonstrated and the technique was successfully applied to demonstrate that GDX is associated with a decrease in poly(A) mRNA content of polyribosomes. Provided that normalization of the hybridization signal for mRNA is achieved with a rRNA cDNA probe, the assay therefore represents a suitable tool for further studies regarding the translational regulation of total and/or specific mRNAs.
Boissonneault
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G
Hormonal Regulation Laboratory, Laval University Hospital Center, Quebec City, Canada.
Tremblay
R R
RR
D000818:Animals; D001284:Atrophy; D015342:DNA Probes; D008297:Male; D008854:Microscopy, Electron; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides; D009919:Orchiectomy; D011132:Polyribosomes / Q000032:analysis*; D011467:Prostate / Q000032:analysis* / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis*; D012335:RNA, Ribosomal / Q000032:analysis*; D012339:RNA, Ribosomal, 28S / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D015342:DNA Probes; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012339:RNA, Ribosomal, 28S; C027903:oligo (dT)