Tissue distribution of 131I radiolabeled transferrin in the athymic nude mouse: localization of a human colon adenocarcinoma HT-29 xenograft.
The tissue distribution of 131I-transferrin (131I-Tf) was studied in athymic nude mice having s.c. human colonic adenocarcinoma HT-29 xenografts. Four days after 131I-Tf injection, the 131I-specific activity measured in the HT-29 tumor, i.e. amount of radioactivity per gram of fresh tissue, represented 0.31 +/- 0.09% of the injected radioactivity and was 1.90 fold more than that measured in the murine colon (P less than 0.05). After correction for intravascular 131I-Tf as estimated by means of 99mTc-Sn in vivo labeling of red blood cells, the 131I specific activity observed in the HT-29 tumor was 7.21 fold more than that observed in the murine colon. This subtracting method enabled us to localize a HT-29 tumor xenograft by gamma scintigraphy of the entire animal and demonstrated that 131I-Tf could be a non-specific but potent marker for human colon cancer.
Prost
A C
AC
Service de Biophysique, C.H.U. La Pitié-Salpêtrière, Paris, France.
Anakök
M
M
Aurengo
A
A
Salomon
J C
JC
Legrand
J C
JC
Rosselin
G
G
D000230:Adenocarcinoma / Q000000981:diagnostic imaging*; D000818:Animals; D003110:Colonic Neoplasms / Q000000981:diagnostic imaging*; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011877:Radionuclide Imaging; D014018:Tissue Distribution; D014168:Transferrin / Q000493:pharmacokinetics*; D014183:Transplantation, Heterologous
D007457:Iodine Radioisotopes; D014168:Transferrin