High performance liquid chromatographic separation of cisplatin and its hydrolysis products on alumina and application to studies of their interaction with cysteine.
An alumina stationary phase has been assessed in the study of the retention behaviour of the anticancer drug cisplatin and its major hydrolysis products. Parameters such as buffer concentration in the mobile phase, pH, organic modifier and competing ion have been investigated in order to optimize chromatographic separation with ultraviolet detection. The separation scheme developed has been used to monitor the hydrolysis of cisplatin in aqueous and saline media, and to monitor the interaction of hydrolysed solutions of cisplatin with the amino acid cysteine. A new peak was observed in the chromatograms of such mixtures when they had been allowed to stand for periods of greater than 16 h and, from analysis of the data obtained, it was concluded that this new peak was due to a complex formed between the mono-aquo hydrolysis product of cisplatin and the amino acid.
Shearan
P
P
School of Chemical Sciences, Dublin City University, Ireland.
Alvarez
J M
JM
Zayed
N
N
Smyth
M R
MR
D000535:Aluminum; D000537:Aluminum Oxide; D002021:Buffers; D002414:Cations, Monovalent; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D002945:Cisplatin / Q000302:isolation & purification*; D003545:Cysteine; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D010710:Phosphates; D012965:Sodium Chloride; D012996:Solutions; D019789:Tetraethylammonium; D013757:Tetraethylammonium Compounds / Q000494:pharmacology; D014867:Water
D002021:Buffers; D002414:Cations, Monovalent; D010710:Phosphates; D012996:Solutions; D013757:Tetraethylammonium Compounds; D014867:Water; D012965:Sodium Chloride; D019789:Tetraethylammonium; D000535:Aluminum; D003545:Cysteine; D000537:Aluminum Oxide; D002945:Cisplatin