title: Affinity of fibroblast growth factors for beta-cyclodextrin tetradecasulfate.
abstract: beta-Cyclodextrin tetradecasulfate was found to have a very strong affinity for fibroblast growth factor (FGF) and could substitute for heparin in FGF purification. Basic FGF was purified about 200,000-fold from a rat chondrosarcoma using a method of biaffinity chromatography in which the beta-cyclodextrin tetradecasulfate polymer was mixed with copper-Sepharose. This method takes advantage of the strong affinity of FGF for both beta-cyclodextrin tetradecasulfate and copper.
authors.affiliation: Department of Surgery, Children's Hospital, Boston, Massachusetts.
authors.forename: S N
authors.initials: SN
authors.lastname: Yuen
authors.forename: J
authors.initials: J
authors.lastname: Folkman
authors.forename: P B
authors.initials: PB
authors.lastname: Weisz
authors.forename: M M
authors.initials: MM
authors.lastname: Joullie
authors.forename: W R
authors.initials: WR
authors.lastname: Ewing
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002846:Chromatography, Affinity / Q000379:methods*; D003505:Cyclodextrins; D003912:Dextrins; D004591:Electrophoresis, Polyacrylamide Gel; D005346:Fibroblast Growth Factors / Q000302:isolation & purification*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D011485:Protein Binding; D051381:Rats; D011994:Recombinant Proteins; D013213:Starch; D047392:beta-Cyclodextrins
chemical_list: D003505:Cyclodextrins; D003912:Dextrins; D011994:Recombinant Proteins; D047392:beta-Cyclodextrins; C064089:beta-cyclodextrin tetradecasulfate; D005346:Fibroblast Growth Factors; D013213:Starch