Autoantibodies, LY-1, and immunoglobulin V gene expression in hybridomas obtained from young and from old New Zealand black mice.
We obtained a large number of hybridomas from 1-month-old and 16-month-old New Zealand black mice to study the fine specificities of the autoantibodies produced, the expression of Ly-1, and the expression of the immunoglobulin V gene families in this autoimmune strain. Analysis of the autoantibody specificities yielded 2 major classifications: those specific for a single autoantigen and those that exhibited multispecific binding. Among the multispecific antibodies, 2 categories were found: an antigen-inhibitable group and an antigen-noninhibitable group. A large proportion of VHJ558 and VH7183 gene families was observed in hybridomas obtained from 1-month-old mice, and in hybridomas obtained from 16-month-old mice, there was a large proportion of VHJ558 and VH36-60 gene families. Among the autoantibody kappa chains secreted by the hybridomas, there was a higher frequency of the V kappa 1, V kappa 8, and V kappa 9 gene families. Autoantibodies were produced by both the Ly-1+ and the Ly-1- B cell subsets.
Fidanza
V
V
Department of Microbiology, Mount Sinai School of Medicine, New York 10029.
Mayer
R
R
Zaghouani
H
H
DiLiberti
M A
MA
Bona
C A
CA
D000367:Age Factors; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000950:Antigens, Ly / Q000032:analysis*; D001323:Autoantibodies / Q000032:analysis*; D005786:Gene Expression Regulation; D006825:Hybridomas / Q000276:immunology*; D007136:Immunoglobulins / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008814:Mice, Inbred NZB; D009928:Organ Specificity
D000911:Antibodies, Monoclonal; D000950:Antigens, Ly; D001323:Autoantibodies; D007136:Immunoglobulins