Detection of adducts of deoxyribonucleic acid in white blood cells of roofers by 32P-postlabeling. Relationship of adduct levels to measures of exposure to polycyclic aromatic hydrocarbons.
To assess the utility of adducts of deoxyribonucleic acid (DNA) as biomarkers of exposure to carcinogens in an industrial population, a pilot study of roofers occupationally exposed to polycyclic aromatic hydrocarbons was conducted. DNA isolated from white blood cells of roofers and nonoccupationally exposed comparison subjects matched for age, sex, and smoking status was analyzed for DNA adducts with the use of 32P-postlabeling methods. Occupational exposures to polycyclic aromatic hydrocarbons were assessed by personal air sampling and skin wipes. Ten of the 12 roofers, but only 2 of the 12 comparison subjects, had detectable levels of aromatic DNA adducts in the 32P-postlabeling assay. Among the roofers, the post-shift levels of polycyclic aromatic hydrocarbons in the skin wipes were correlated with the DNA adduct levels. These results suggest that 32P-postlabeling assay may be useful for monitoring internal exposures to complex mixtures of aromatic hydrocarbons in industrial populations.
Herbert
R
R
Department of Community Medicine, Mount Sinai Medical Center, New York, New York 10029-6574.
Marcus
M
M
Wolff
M S
MS
Perera
F P
FP
Andrews
L
L
Godbold
J H
JH
Rivera
M
M
Stefanidis
M
M
Lu
X Q
XQ
Landrigan
P J
PJ
D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood; D015331:Cohort Studies; D004247:DNA / Q000378:metabolism*; D004781:Environmental Exposure; D004812:Epidemiologic Methods; D006801:Humans; D007553:Isotope Labeling; D007962:Leukocytes; D008175:Lung Neoplasms / Q000453:epidemiology / Q000209:etiology; D008297:Male; D008875:Middle Aged; D010761:Phosphorus Radioisotopes; D010865:Pilot Projects; D011083:Polycyclic Compounds / Q000009:adverse effects / Q000378:metabolism*; D012878:Skin Neoplasms / Q000453:epidemiology / Q000209:etiology
D014408:Biomarkers, Tumor; D010761:Phosphorus Radioisotopes; D011083:Polycyclic Compounds; D004247:DNA