title: Microassay for the simultaneous determination of cocaine, norcocaine, benzoylecgonine and benzoylnorecgonine by high-performance liquid chromatography.
abstract: An improved method for the simultaneous determination of cocaine, norcocaine, benzoylecgonine and benzoylnorecgonine using reversed-phase high-performance liquid chromatography with ultraviolet detection is described. Following solid-phase extraction, chromatography was performed using a column containing an octadecylsilica-coated packing, eluted with 6% acetonitrile in phosphate buffer, pH 2.1, and detected at 233 nm. Using 80-microliters samples, the detection limit is 18 ng/ml for benzoylecgonine and benzoylenorecgonine and 35 ng/ml for cocaine and norcocaine. The coefficients of variation range from 3.5% (benzoylecgonine) to 7.0% (norcocaine). The procedure has been applied to samples of guinea pig plasma, urine and amniotic fluid and human urine.
authors.affiliation: Department of Pharmacology, School of Medicine, Oregon Health Sciences University, Portland 97201.
authors.forename: J A
authors.initials: JA
authors.lastname: Sandberg
authors.forename: G D
authors.initials: GD
authors.lastname: Olsen
mesh_terms: D000653:Amniotic Fluid / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003042:Cocaine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood / Q000652:urine; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006168:Guinea Pigs; D008431:Maternal-Fetal Exchange; D011247:Pregnancy
chemical_list: C008671:norcocaine; C005618:benzoylecgonine; D003042:Cocaine; C012209:benzoylnorecgonine