Comparative study of three fluorescent dyes for angiography: sodium fluorescein, carboxyfluorescein, and calcein.
To compare the characteristics of carboxyfluorescein (CF) and calcein (Calc) with those of sodium fluorescein (Naf), the only fluorescent dye currently in clinical use, we performed angiography in rabbits and primates using these three dyes. The circulation decay time of all dyes was longer in primates than in rabbits. In primates, CF and Calc had longer decay times than Naf. Calc produced the greatest contrast between the choroidal and retinal vasculature. Tissue staining and dye leakage into the vitreous immediately after retinal photocoagulation were minimal with Calc, moderate with CF, and marked with Naf. The limited leakage and longer circulation time of Calc may permit simultaneous angiography and photocoagulation therapy without obscuring the fundus view with leaking dye from the photocoagulated structure.
Fang
T
T
LSU Eye Center, Louisiana State University Medical Center School of Medicine, New Orleans 70112.
Naguib
K S
KS
Peyman
G A
GA
Khoobehi
B
B
D000792:Angiography; D000818:Animals; D002829:Choroid / Q000098:blood supply* / Q000000981:diagnostic imaging; D019793:Fluorescein; D005452:Fluoresceins / Q000493:pharmacokinetics*; D005654:Fundus Oculi; D008028:Light Coagulation; D015394:Molecular Structure; D010539:Permeability; D011817:Rabbits; D012171:Retinal Vessels / Q000000981:diagnostic imaging*; D012453:Saimiri; D014822:Vitreous Body / Q000378:metabolism
D005452:Fluoresceins; C024098:6-carboxyfluorescein; D019793:Fluorescein; C007740:fluorexon