Growth of human keratinocytes in a defined medium supplemented with growth factor of serum.
In order to prepare a good medium for cultivation of keratinocytes, bovine serum was fractionated by precipitation with various concentrations of ammonium sulfate. The fractionated serum proteins were added to the basal medium at a concentration of 3 mg/ml. The best proliferation was observed in the medium supplemented with the 55-70% ammonium sulfate precipitated fraction. No growth of keratinocytes was observed when the medium was supplemented with the 0-35% fraction. The growth promotion by the 55-70% fraction was completely inhibited by the 0-35% fraction. These results have shown that the fractionation of serum protein clearly separated the growth-inhibitory factors and the growth-stimulating factors.
Kitano
Y
Y
Department of Dermatology, Hyogo College of Medicine, Nishinomiya, Japan.
Okada
N
N
Sasai
S
S
D001798:Blood Proteins / Q000502:physiology*; D002448:Cell Adhesion; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D006133:Growth Substances / Q000502:physiology*; D006801:Humans; D015603:Keratinocytes / Q000502:physiology*
D001798:Blood Proteins; D003470:Culture Media; D006133:Growth Substances