title: Kinetics of a multistate enzyme in a large oscillating field.
abstract: A simple, general, and efficient method for calculating the response of a set of coupled first-order (or pseudo-first-order) chemical reactions to an arbitrarily large periodic field is described. The method is applied to a four-state membrane transport enzyme that is electroconformationally coupled to an ac field, i.e., the enzyme has electric charges that move concomitantly with a conformational transition. The calculation is done both for enzymes in a planar membrane and for enzymes in the spherical membrane of a cell or vesicle in suspension.
authors.affiliation: Chemical Process Metrology Division, National Institute of Standards and Technology, Gaithersburg, Maryland 20899.
authors.forename: B
authors.initials: B
authors.lastname: Robertson
authors.forename: R D
authors.initials: RD
authors.lastname: Astumian
mesh_terms: D004798:Enzymes / Q000378:metabolism*; D007700:Kinetics; D008433:Mathematics; D008962:Models, Theoretical; D009991:Oscillometry; D011487:Protein Conformation; D013816:Thermodynamics
chemical_list: D004798:Enzymes