Immobilization of small molecules and proteins by radio-derivatized polystyrene.
When molded polystyrene (PS) products (e.g., microtiter plates) or latex particles are irradiated with high-energy (1-10 Mrads) gamma rays in the presence of nonpolymerizable small molecules such as aromatic amines, some of these molecules incorporate into PS, which leads to the formation of radio-derivatized PS (RDPS). Two classes of RDPS can be identified regarding their ability for immobilization of biologically important molecules: 1) reactive RDPS that are able to form covalent bonds with molecules such as proteins without the help of cross-linkers, and 2) functionalized RDPS that can be used for the immobilization of molecules with activators (e.g., carbodiimides) or cross-linkers. The method can be used for the production of low-noise supports for binding assays. Most of the RDPS can be produced without impairment of the optical quality of PS, making derivatized microtiter plates suitable for colorimetric assays. The principle can be applied for the preparation of affinity sorbents, e.g., for high-performance affinity chromatography and for the immobilization of enzymes using latex PS particles.
Varga
J M
JM
Department of Dermatology, University of Innsbruck, Austria.
Fritsch
P
P
D000588:Amines; D002250:Carbon Radioisotopes; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D005720:Gamma Rays; D006454:Hemoglobins; D011137:Polystyrenes; D011506:Proteins; D012260:Ribonucleases; D014316:Tritium
D000588:Amines; D002250:Carbon Radioisotopes; D003432:Cross-Linking Reagents; D004800:Enzymes, Immobilized; D006454:Hemoglobins; D011137:Polystyrenes; D011506:Proteins; D014316:Tritium; D012260:Ribonucleases