Chemosensitivity testing of human gliomas using a fluorescent microcarrier technique.
This report describes a new fluorescent microcarrier cytostasis assay. Human glioma cell lines and primary cultures were attached to microcarrier tissue culture beads and treated with various chemotherapeutic drugs. After treatment, the cells were labelled with two vital fluorescent dyes in order to measure cellular viability. The uptake of hydroethidine and Hoechst 33342 was evaluated alone and in combination as probes for determining metabolic activity and cellular proliferation. Hydroethidine was found to be superior when compared to trypan blue and tritiated thymidine. The use of the microcarrier technique allows for the direct cellular measurement of fluorescence without the need of extensive extraction procedures. The fluorescent assay is a sensitive, rapid and an effective way to screen for potential antiproliferative compounds.
Bowles
A P
AP
Department of Surgery, Medical College of Georgia, Augusta 30912.
Pantazis
C G
CG
Wansley
W
W
Allen
M B
MB
D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use; D001562:Benzimidazoles / Q000493:pharmacokinetics; D002330:Carmustine / Q000494:pharmacology / Q000627:therapeutic use*; D002455:Cell Division / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D005456:Fluorescent Dyes; D005910:Glioma / Q000188:drug therapy*; D006801:Humans; D010617:Phenanthridines / Q000493:pharmacokinetics; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000970:Antineoplastic Agents; D001562:Benzimidazoles; D005456:Fluorescent Dyes; D010617:Phenanthridines; C058428:hydroethidine; C017807:bisbenzimide ethoxide trihydrochloride; D002330:Carmustine