Simple and rapid purification of brevin.
Brevin or plasma gelsolin, a calcium dependent actin-binding and actin-severing protein, was purified from bovine plasma by a very rapid and simple procedure; ammonium sulfate fractionation and only one step of anion exchange column chromatography by a convenient use. It takes only 24 hrs to complete all the procedure. The purity of brevin prepared by this method was more than 95% on SDS-PAGE and total recovery was much better than previous preparation methods. This brevin preparation has about 8 isomers on 2-D PAGE and strong severing activity on F-actin under electron microscopic observation.
Kurokawa
H
H
Department of Pharmacology, Faculty of Medicine, University of Tokyo, Japan.
Fujii
W
W
Ohmi
K
K
Sakurai
T
T
Nonomura
Y
Y
D000199:Actins / Q000648:ultrastructure; D000645:Ammonium Sulfate; D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange; D005069:Evaluation Studies as Topic; D018260:Gelsolin; D007526:Isoelectric Point; D007536:Isomerism; D008840:Microfilament Proteins / Q000097:blood / Q000302:isolation & purification* / Q000648:ultrastructure
D000199:Actins; D018260:Gelsolin; D008840:Microfilament Proteins; C016953:brevin; D000645:Ammonium Sulfate