Quantitation of hexadecylphosphocholine by high-performance thin-layer chromatography with densitometry.
A method for the determination of the antineoplastic ether phospholipid hexadecylphosphocholine (HePC) is presented, based on the separation of the lipids by high-performance thin-layer chromatography charring with a cupric sulphate reagent and quantitation by in situ densitometry. The lower limit of determination is ca. 25 pmol. Concentrated hexane-isopropanol extracts of plasma samples can be applied to the plate without further clean-up, making this method useful for clinical drug monitoring. Additional ion-exchange chromatography and removal of the salt contaminants by gel filtration permits the study of endogenous phospholipids together with HePC from the same sample.
Rustenbeck
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Institute of Pharmacology and Toxicology, University of Göttingen, F.R.G.
Lenzen
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S
D002794:Choline / Q000031:analogs & derivatives*; D002855:Chromatography, Thin Layer / Q000379:methods*; D003720:Densitometry / Q000379:methods; D006801:Humans; D008055:Lipids / Q000032:analysis / Q000097:blood; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood
D008055:Lipids; D010767:Phosphorylcholine; C039128:miltefosine; D002794:Choline