Anaphylactic properties of monohaptenic dinitrophenylated tripalmitoyl-S-glyceryl-cysteinyl lipopeptides.
Tripalmitoyl-S-glycerylcysteinyl lipopeptides are B-cell and macrophage activating and may be used as low molecular weight immunogens of considerable potency and even as vaccines when conjugated with suitable epitopic structures. Selected lipopeptides carrying single Dnp haptens were found to evoke mild passive cutaneous anaphylaxis in guinea pigs sensitized against Dnp. The reactions were observed after intravenous injection whereas intradermally applied antigen was negative. The anaphylactogenicity seems unrelated to micelle or aggregate formation of the insoluble peptides which require lecithin additions as well as sonication to become solubilized. The dinitrophenylated lipopeptide tripalmitoyl-S-glyceryl-cysteinyl-seryl-lysine produced toxic reactions which were not observed with the lipopeptide devoid of Dnp. Dinitrophenylated tripalmitoyl-S-glycerylcysteiny-1,6-diaminohexane and tripalmitoyl-S-glyceryl-cysteinyl-lysine did not show these toxic reactions.
Schneider
C H
CH
Institute for Clinical Immunology, University of Berne, Inselspital, Switzerland.
Rolli
H
H
Metzger
J
J
Jung
G
G
D000818:Animals; D004136:Dinitrobenzenes / Q000008:administration & dosage / Q000276:immunology*; D006168:Guinea Pigs; D006241:Haptens / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D007271:Injections, Intradermal; D007275:Injections, Intravenous; D008074:Lipoproteins / Q000008:administration & dosage / Q000276:immunology*; D009578:Nitrobenzenes / Q000276:immunology*; D010323:Passive Cutaneous Anaphylaxis / Q000276:immunology*; D011233:Precipitin Tests; D014280:Triglycerides / Q000008:administration & dosage / Q000276:immunology*
D004136:Dinitrobenzenes; D006241:Haptens; D007106:Immune Sera; D008074:Lipoproteins; D009578:Nitrobenzenes; D014280:Triglycerides