Pouring wide-range immobilized pH gradient gels with a window of extremely flattened slope.
A gradient mixing device has been designed to pour polyacrylamide gels with a wide-range immobilized pH gradient including a "window" of extremely flattened slope. The device consists of an IBM-compatible personal computer controlling 8 step-motor-driven burettes, with four producing a density gradient from glycerol and delivering acrylamides and catalysts for gel polymerization, two delivering Immobilines for a wide-range pH gradient and the other two burettes responsible for the delivery of Immobilines for a partial pH range inside the wide range. The effect on a complex separation pattern of proteins with a wide range of pI is that resolution can be increased reproducibly to any reasonable extent at any location of the separation pattern.
Altland
K
K
Institute of Human Genetics, Justus-Liebig-University of Giessen, Federal Republic of Germany.
Altland
A
A
D000178:Acrylamides; D000180:Acrylic Resins; D003201:Computers; D005990:Glycerol; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing / Q000295:instrumentation*; D007526:Isoelectric Point; D011228:Prealbumin / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification
D000178:Acrylamides; D000180:Acrylic Resins; D011228:Prealbumin; D011506:Proteins; C016680:polyacrylamide gels; C059413:Immobiline; D005990:Glycerol