title: Homograft valve durability: host or donor influence?
abstract: Antibiotic sterilized valves have been shown to function longer than those chemically sterilized; however, the reason remains obscure. Current hypotheses cite either retention of donor fibroblasts capable of repairing the grafted valve, or host fibroblast ingrowth into and onto the leaflet ground substance. A cryopreserved aortic homograft from a male donor was explanted from a female recipient after 10 months, and subjected to immunocytochemistry, tissue culture, and karyotyping. The leaflet bases exhibited normal morphology with an intact endothelium. The distal one-third of the leaflets was devoid of fibroblasts from the leaflet bases showed them to be of host origin. This homograft seems to have been implanted with an intact ground substance which allowed for host cell repopulation of the inner one-third of the leaflets. Perhaps donor cell viability in itself is not as important to durability as is preservation of the leaflet ground substance, but rather the presence of viable cells may be an index of the structural integrity of the collagen and elastic matrix.
authors.affiliation: Robert Wood Johnson University Hospital, New Brunswick, NJ 089023-0019.
authors.forename: L
authors.initials: L
authors.lastname: Gonzalez-Lavin
authors.forename: A J
authors.initials: AJ
authors.lastname: Spotnitz
authors.forename: J W
authors.initials: JW
authors.lastname: Mackenzie
authors.forename: J
authors.initials: J
authors.lastname: Gu
authors.forename: I K
authors.initials: IK
authors.lastname: Gadi
authors.forename: J
authors.initials: J
authors.lastname: Gullo
authors.forename: C
authors.initials: C
authors.lastname: Boyd
authors.forename: D
authors.initials: D
authors.lastname: Graf
mesh_terms: D000328:Adult; D001021:Aortic Valve / Q000473:pathology / Q000637:transplantation*; D001022:Aortic Valve Insufficiency / Q000151:congenital / Q000473:pathology / Q000601:surgery*; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts; D006801:Humans; D007150:Immunohistochemistry; D007621:Karyotyping; D012086:Reoperation; D014184:Transplantation, Homologous / Q000473:pathology / Q000502:physiology*