Platelet derived growth factor inhibits 5 alpha-reductase and delta 5-3 beta-hydroxysteroid dehydrogenase activities in cultured immature Leydig cells.
Platelet derived growth factor inhibited both hCG- and 8-Br-cAMP-stimulated 5 alpha-reductase activity in cultured immature Leydig cells in a dose-dependent manner, while not significantly inhibiting basal enzyme activity. Platelet derived growth factor also inhibited basal delta 5-3 beta-hydroxysteroid dehydrogenase activity and hCG-stimulated testosterone formation. Maximal inhibitions were achieved with 10 ng/ml of platelet derived growth factor. These studies suggest that platelet derived growth factor should be included among the variety of locally produced regulatory factors which modulate Leydig cell function.
Murono
E P
EP
Research Service, Dorn Veterans' Hospital, Columbia, SC.
Washburn
A L
AL
D015096:3-Hydroxysteroid Dehydrogenases / Q000037:antagonists & inhibitors*; D058891:5-alpha Reductase Inhibitors; D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D007985:Leydig Cells / Q000201:enzymology*; D008297:Male; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D051381:Rats; D013739:Testosterone / Q000096:biosynthesis
D058891:5-alpha Reductase Inhibitors; D006063:Chorionic Gonadotropin; D010982:Platelet-Derived Growth Factor; D015124:8-Bromo Cyclic Adenosine Monophosphate; D013739:Testosterone; D015096:3-Hydroxysteroid Dehydrogenases; C031954:delta(5)-3 beta-hydroxysteroid dehydrogenase