title: Membrane contact, fusion, and hexagonal (HII) transitions in phosphatidylethanolamine liposomes.
abstract: The behavior of phosphatidylethanolamine (PE) liposomes has been studied as a function of temperature, pH, ionic strength, lipid concentration, liposome size, and divalent cation concentration by differential scanning calorimetry (DSC), by light scattering, by assays measuring liposomal lipid mixing, contents mixing, and contents leakage, and by a new fluorometric assay for hexagonal (HII) transitions. Liposomes were either small or large unilamellar, or multilamellar. Stable (impermeable, nonaggregating) liposomes of egg PE (EPE) could be formed in isotonic saline (NaCl) only at high pH (greater than 8) or at lower pH in the presence of low ionic strength saline (less than 50 mOsm). Bilayer to hexagonal (HII) phase transitions and gel to liquid-crystalline transitions of centrifuged multilamellar liposomes were both detectable by DSC only at pH 7.4 and below. The HII transition temperature increased, and the transition enthalpy decreased, as the pH was raised above 7.4, and it disappeared above pH 8.3 where PE is sufficiently negatively charged. HII transitions could be detected at high pH following the addition of Ca2+ or Mg2+. No changes in light scattering and no lipid mixing, mixing of contents, or leakage of contents were noted for EPE liposomes under nonaggregating conditions (pH 9.2 and 100 mM Na+ or pH 7.4 and 5 mM Na+) as the temperature was raised through the HII transition region. However, when aggregation of the liposomes was induced by addition of Ca2+ or Mg2+, or by increasing [Na+], it produced sharp increases in light scattering and in leakage of contents and also changes in fluorescent probe behavior in the region of the HII transition temperature (TH). Lipid mixing and contents mixing were also observed below TH under conditions where liposomes were induced to aggregate, but without any appreciable leakage of contents. We conclude that HII transitions do not occur in liposomes under conditions where intermembrane contacts do not take place. Moreover, fusion of PE liposomes at a temperature below TH can be triggered by H+, Na+, Ca2+, or Mg2+ or by centrifugation under conditions that induce membrane contact. There was no evidence for the participation of HII transitions in these fusion events.
authors.affiliation: Cancer Research Institute, University of California, San Francisco 94143.
authors.forename: T M
authors.initials: TM
authors.lastname: Allen
authors.forename: K
authors.initials: K
authors.lastname: Hong
authors.forename: D
authors.initials: D
authors.lastname: Papahadjopoulos
mesh_terms: D002118:Calcium; D002151:Calorimetry; D006863:Hydrogen-Ion Concentration; D008081:Liposomes; D008274:Magnesium; D008962:Models, Theoretical; D008968:Molecular Conformation; D010714:Phosphatidylethanolamines; D012964:Sodium; D013050:Spectrometry, Fluorescence
chemical_list: D008081:Liposomes; D010714:Phosphatidylethanolamines; D012964:Sodium; D008274:Magnesium; D002118:Calcium