An enzymatic colorimetric method for measuring naringin using 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid)(ABTS) in the presence of peroxidase.
A sensitive colorimetric method for naringin estimation using 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) as peroxidase substrate is described. The method is based on the coupling reaction of an ABTS radical cation with an oxidation product of naringin formed by peroxidase. This coupling reaction leads to the formation of a purple-colored compound with a maximum absorption at 560 nm. A molar absorption coefficient at this wavelength was calculated to be 13,286 +/- 200 M-1 cm-1. The lowest amount of naringin that can be detected is 1 nmol. The application of this method to the quantification of naringin in grapefruit tissues is presented.
Arnao
M B
MB
Departamento de Biología Vegetal, Universidad de Murcia, Spain.
Casas
J L
JL
del Río
J A
JA
Acosta
M
M
García-Cánovas
F
F
D052160:Benzothiazoles; D003124:Colorimetry / Q000379:methods; D044950:Flavanones; D005419:Flavonoids / Q000032:analysis*; D005638:Fruit; D006861:Hydrogen Peroxide; D007202:Indicators and Reagents; D010084:Oxidation-Reduction; D010544:Peroxidases; D013053:Spectrophotometry; D013451:Sulfonic Acids
D052160:Benzothiazoles; D044950:Flavanones; D005419:Flavonoids; D007202:Indicators and Reagents; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D006861:Hydrogen Peroxide; D010544:Peroxidases; C005274:naringin