Cyclobutane-pyrimidine dimer excision in UV-sensitive CHO mutants and the effect of the human ERCC2 repair gene.
Using a radiochromatographic assay, we have examined cis-syn cyclobutane-pyrimidine dimer removal after ultraviolet irradiation in cell lines representative of the first 6 complementation groups of Chinese hamster ovary DNA nucleotide excision repair mutants. AA8, the CHO cell line from which these mutants were derived, consistently showed normal dimer excision for a rodent cell. The mutants uniformly exhibited no significant dimer excision within the limits of determination. Additionally, V-H1, a mutant belonging to complementation group 2 and derived from V79 hamster cells, exhibited no dimer excision. Two UV5 derived transformants that carry the complementing human ERCC2 repair gene showed a capacity for dimer excision comparable to the AA8 wild-type cells.
Regan
J D
JD
Biology Division, Oak Ridge National Laboratory, TN 37831.
Thompson
L H
LH
Carrier
W L
WL
Weber
C A
CA
Francis
A A
AA
Zdzienicka
M Z
MZ
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004260:DNA Repair; D004269:DNA, Bacterial / Q000528:radiation effects*; D005796:Genes; D006801:Humans; D009154:Mutation; D011740:Pyrimidine Dimers / Q000235:genetics*; D014170:Transformation, Genetic; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D011740:Pyrimidine Dimers