title: Cooperativity between platelet-activating factor and collagen in aggregation of bovine platelets, II.
abstract: When subthreshold amounts of platelet-activating factor (PAF) and collagen were added simultaneously, strong aggregation of platelets was induced. However, each agonist alone at these concentrations could not induce aggregation at all (S. Kojima et al, (1987) Biochem. Biophys. Res. Commun. 145, 915-920). This cooperativity was observed not only in aggregation but also in changes of intracellular Ca2+ concentration, 47 kDa protein phosphorylation, and formation of thromboxane. These findings suggest that various steps of signal transduction pathways are enhanced during their cooperativity.
authors.affiliation: Department of Biological Sciences, Tokyo Institute of Technology, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kojima
authors.forename: F
authors.initials: F
authors.lastname: Sekiya
authors.forename: Y
authors.initials: Y
authors.lastname: Inada
authors.forename: T
authors.initials: T
authors.lastname: Tsukada
authors.forename: Y
authors.initials: Y
authors.lastname: Saito
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D003094:Collagen / Q000494:pharmacology*; D004357:Drug Synergism; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D010766:Phosphorylation; D010972:Platelet Activating Factor / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*; D015398:Signal Transduction; D013931:Thromboxanes / Q000096:biosynthesis
chemical_list: D010972:Platelet Activating Factor; D013931:Thromboxanes; D003094:Collagen; D002118:Calcium