title: Cloning and heterospecific expression of the resistance determinant vanA encoding high-level resistance to glycopeptides in Enterococcus faecium BM4147.
abstract: Fragments of plasmid pIP816, which confers high-level glycopeptide resistance in Enterococcus faecium BM4147, were cloned into a conjugative gram-negative-gram-positive shuttle vector. The resulting hybrids were transferred by conjugation from Escherichia coli to Enterococcus faecalis and Bacillus thuringiensis. A 4-kilobase EcoRI fragment from pIP816 was found to confer vancomycin resistance in these hosts but not in E. coli or Bacillus subtilis.
authors.affiliation: Unité des Agents Antibactériens, Unité Associée Centre National de la Recherche Scientifique 271, Institut Pasteur, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Brisson-Noël
authors.forename: S
authors.initials: S
authors.lastname: Dutka-Malen
authors.forename: C
authors.initials: C
authors.lastname: Molinas
authors.forename: R
authors.initials: R
authors.lastname: Leclercq
authors.forename: P
authors.initials: P
authors.lastname: Courvalin
mesh_terms: D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004352:Drug Resistance, Microbial; D015964:Gene Expression Regulation, Bacterial; D006020:Glycopeptides / Q000494:pharmacology*; D010957:Plasmids; D013291:Streptococcus / Q000187:drug effects / Q000235:genetics*; D014640:Vancomycin / Q000494:pharmacology*
chemical_list: D006020:Glycopeptides; D014640:Vancomycin