The effect of platelet-activating factor on [14C]aminopyrine uptake by isolated guinea pig parietal cells.
C16- and C18- platelet-activating factor (PAF) at 10(-6) M enhanced the uptake of [14C]aminopyrine (AP) by isolated guinea pig parietal cells. This increase was inhibited by a PAF antagonist CV-6209. In a medium with a low calcium (Ca2+) concentration (2 uM), this increase was not observed, which indicates that the increase of AP uptake by PAF is dependent on extracellular Ca2+. PAF and lyso-PAF showed no effect on AP uptake in the presence of histamine or carbachol.
Nogami
M
M
Department of Medicine and Physical Therapy, Faculty of Medicine, University of Tokyo, Japan.
Suko
M
M
Miyamoto
T
T
D000632:Aminopyrine / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002217:Carbachol; D005744:Gastric Acid / Q000378:metabolism; D006168:Guinea Pigs; D006632:Histamine; D066298:In Vitro Techniques; D008297:Male; D010295:Parietal Cells, Gastric / Q000378:metabolism*; D010972:Platelet Activating Factor / Q000031:analogs & derivatives / Q000494:pharmacology / Q000502:physiology*; D011726:Pyridinium Compounds
C029271:O-deacetyl platelet activating factor; D010972:Platelet Activating Factor; D011726:Pyridinium Compounds; D000632:Aminopyrine; C053321:CV 6209; D006632:Histamine; D002217:Carbachol; D002118:Calcium