title: The difference in the type of codon-anticodon base pairing at the ribosomal P-site is one of the determinants of the translational rate.
abstract: By utilizing an enzymatically reconstructed tRNA variant containing an altered anticodon sequence, we have examined the different biochemical behavior of translation between the Watson-Crick type and the wobble type base pair interactions at the first anticodon position. We have found that the Watson-Crick type base pair has an advantage in translation in contrast to the wobble type base pair by comparing the efficiency of transpeptidation of native tRNA(Phe) (anticodon; GmAA) with its variant tRNA (anticodon; AAA) in the poly(U)-programmed ribosome system. Thomas et al. [Proc. Natl. Acad. Sci. U.S. (1988) 85, 4242-4246] showed that the wobble codon at the ribosomal A-site accepted its cognate tRNA less efficiently than the Watson-Crick base pairing codon. We report here that the wobble interaction at the ribosomal P-site also affected the rate of translation. This variable translational rate may be a mechanism of gene regulation through preferential codon usage.
authors.affiliation: Department of Molecular Biology, School of Science, Nagoya University, Aichi.
authors.forename: M
authors.initials: M
authors.lastname: Kato
authors.forename: K
authors.initials: K
authors.lastname: Nishikawa
authors.forename: M
authors.initials: M
authors.lastname: Uritani
authors.forename: M
authors.initials: M
authors.lastname: Miyazaki
authors.forename: S
authors.initials: S
authors.lastname: Takemura
mesh_terms: D000926:Anticodon / Q000378:metabolism*; D001482:Base Composition; D001483:Base Sequence; D003062:Codon / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D012360:RNA, Transfer, Phe / Q000138:chemical synthesis; D015003:Yeasts
chemical_list: D000926:Anticodon; D003062:Codon; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012360:RNA, Transfer, Phe; D012343:RNA, Transfer