A new powerful association for immunoassay: organometallic label conjugated to solvent separation method.
The convenient use of solvents as a method to separate free (F) and bound (B) fractions in drug immunoassays which utilize organometallic moieties as labels, has been developed. Two types of drugs labelled with an organometallic complex and denominated metallohaptens (or metallotracers) are studied as model assays: desipramine (tricyclic antidepressant) and phenobarbital (antiepileptic agent). Different organic solvents have potentialities to extract these metallohaptens. To illustrate this solvent procedure we describe antiserum dilution curves and we compare the results with those obtained for the same samples using radiotracers in conjunction with dextran coated charcoal as separation agent. This immunoassay method which associates a metallotracer and B/F solvent separation appears to be fast, cheap, without the step of centrifugation.
Mariet
F
F
Unité d'immunoanalyse, Faculté de Pharmacie, Dijon, France.
Brossier
P
P
D003891:Desipramine / Q000032:analysis*; D007118:Immunoassay / Q000379:methods*; D015394:Molecular Structure; D009942:Organometallic Compounds; D010634:Phenobarbital / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods; D012997:Solvents
D009942:Organometallic Compounds; D012997:Solvents; D003891:Desipramine; D010634:Phenobarbital