Characteristics and in vivo occurrence of type VIII collagen.
Type VIII collagen was isolated from bovine Descemet's membranes by pepsin treatment and salt fractionation, as described by Kapoor et al. [(1986) Biochemistry 25, 3930-3937]. Contaminating type IV collagen was removed by ion-exchange chromatography. Purified type VIII collagen consisted of two different polypeptide chains and, compared to the fiber forming collagens, showed a higher thermal stability. Corresponding fractions isolated from pepsinized human Ewing's sarcoma and fetal calf aorta reacted immunologically with a protein of similar molecular mass. After extraction of Descemet's membranes with guanidine hydrochloride, a peptide of about 60 kDa was obtained. This seems to be the tissue form of type VIII collagen.
Jander
R
R
Institut für Arterioskleroseforschung an der Universität Münster, Federal Republic of Germany.
Korsching
E
E
Rauterberg
J
J
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001011:Aorta / Q000032:analysis / Q000196:embryology; D001485:Basement Membrane / Q000032:analysis; D002417:Cattle; D002852:Chromatography, Ion Exchange; D003094:Collagen / Q000302:isolation & purification* / Q000648:ultrastructure; D003315:Cornea / Q000032:analysis; D006801:Humans; D015151:Immunoblotting; D008854:Microscopy, Electron; D008958:Models, Molecular; D012512:Sarcoma, Ewing / Q000032:analysis; D014357:Trypsin
D000596:Amino Acids; D003094:Collagen; D014357:Trypsin