Studies on the uptake of N-methylisoquinolinium ion into rat striatal slices using high-performance liquid chromatography with fluorimetric detection.
A simple and sensitive procedure for the measurement of N-methylisoquinolinium ion (NMIQ+), a putative neurotoxin, was devised using high-performance liquid chromatography (HPLC) with fluorescence detection. Separation of NMIQ+ was carried out by gel filtration and reversed-phase HPLC on a column of hydrophilic polymer gels (Asahipak GS-302H). The method was sensitive enough to measure 50 fmol of NMIQ+. Uptake of NMIQ+ into rat striatal slices was confirmed by this method.
Hirata
Y
Y
Department of Hygiene and Public Health, Nippon Medical School, Tokyo, Japan.
Minami
M
M
Naoi
M
M
Nagatsu
T
T
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003342:Corpus Striatum / Q000378:metabolism*; D005453:Fluorescence; D008297:Male; D008996:Monoamine Oxidase Inhibitors / Q000378:metabolism / Q000493:pharmacokinetics*; D011806:Quinolinium Compounds / Q000378:metabolism / Q000493:pharmacokinetics*; D051381:Rats; D011919:Rats, Inbred Strains
D008996:Monoamine Oxidase Inhibitors; D011806:Quinolinium Compounds; C051351:N-methylisoquinolinium