Repetition structure of mammalian nuclear DNA.
We have used Fragmentation Sequencing logic to analyse the repetition structure of several large human genomic genes. The method, based on a proposed laboratory scheme for DNA sequencing, detects short sequences which are repeated near, but not necessarily adjacent, to each other (cryptically simple DNA). We find a low frequency of such repeats. There is a slight excess of such repeats in introns over exons, and a slight but significant excess in genomic DNA over random DNA, confirming that cryptically simple sequences are over-represented in the genome. The analysis suggests that Fragmentation Sequencing will be a suitable method for sequencing large mammalian genes.
Smillie
F
F
Department of Biochemistry, University of Bath, Claverton Down, U.K.
Bains
W
W
D000818:Animals; D001483:Base Sequence; D004247:DNA; D005340:Fibrinogen / Q000235:genetics; D015894:Genome, Human; D006801:Humans; D008322:Mammals / Q000235:genetics; D008957:Models, Genetic; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*
D005340:Fibrinogen; D004247:DNA