An erythrocyte glycoprotein of apparent Mr 60,000 expresses the Sc1 and Sc2 antigens.
Immunoblotting with human anti-Sc1 and anti-Sc2 locates the Sc1 and Sc2 antigens to an erythrocyte membrane glycoprotein of apparent Mr 60,000. The antigens are destroyed by pronase, and require intact disulphide bonds for expression. A proportion of the molecules carrying the Sc1 and Sc2 antigens are associated with red cell cytoskeleton preparations. Treatment of intact cells with an Endo F preparation resulted in the loss of the Sc2 antigen but not the Sc1 antigen, suggesting that the Sc2 antigen is dependent on the presence of one or more complex N-glycans for its expression.
Spring
F A
FA
South Western Regional Blood Transfusion Centre, Southmead, Bristol, UK.
Herron
R
R
Rowe
G
G
D001789:Blood Group Antigens / Q000276:immunology*; D004910:Erythrocyte Membrane / Q000276:immunology*; D006801:Humans; D008562:Membrane Glycoproteins / Q000276:immunology*; D008970:Molecular Weight
D001789:Blood Group Antigens; D008562:Membrane Glycoproteins