Evaluation of cytotoxic cell-target cell conjugates. Comparison between flow cytometric analysis and 51Cr binding assay.
Two methods for evaluating the conjugate formation between either bulk cultured or cloned NK cells and fresh tumor target cells or tumor cell lines were compared. Flow cytometric analysis and 51Cr binding assay yielded comparable results using NK clones as effector cells; however, flow cytometry provides less accuracy using large granular lymphocytes or unfractionated lymphocytes.
Heltai
S
S
Istituto Scientifico San Raffaele, Milano, Italy.
Poggi
A
A
Sabbadini
M G
MG
Zocchi
M R
MR
D002860:Chromium Radioisotopes; D003602:Cytotoxicity, Immunologic / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D014407:Tumor Cells, Cultured
D002860:Chromium Radioisotopes