title: Hypothermia, hepatic oxygen supply-demand, and ischemia-reperfusion injury in pigs.
abstract: We examined the effects of two degrees of hypothermia on hepatic oxygen delivery and uptake, hepatic lactate uptake as a marker of hepatic function, and the effect of hypothermia on ischemia-reperfusion injury in the liver in miniature pigs (n = 18, 21-30 kg body wt). Hepatic arterial and portal venous blood flows were measured while hepatic oxygen delivery was progressively decreased without venous congestion in the preportal area. With decreases in hepatic blood and oxygen supply, oxygen extraction gradually increased from 50 to 90% in the normothermic group and from 25 to 70 and 84% in the hypothermic (30. and 34 degrees C, respectively) groups. The values of critical hepatic oxygen delivery were between 7.3 and 11.9 ml O2.min-1.100 g-1 without significant differences among the groups. During reperfusion after ischemic insult, hepatic oxygen uptake returned to base-line values in both hypothermic groups but remained substantially below base-line values in normothermic groups of animals. Hepatic enzyme concentrations (lactate dehydrogenase, alanine aminotransferase, aspartate aminotransferase, and alcohol dehydrogenase) were substantially increased (up to 30-fold) in normothermic animals, but the concentrations did not increase in either of the hypothermic groups. These results demonstrated that hypothermia per se does not affect hepatic oxygen delivery but decreases hepatic oxygen demand and uptake, provides an effective protection from hepatic oxygen deprivation, and lessens reperfusion injury.
authors.affiliation: Department of Anesthesiology, University of Alabama, Birmingham 35233.
authors.forename: K
authors.initials: K
authors.lastname: Nagano
authors.forename: S
authors.initials: S
authors.lastname: Gelman
authors.forename: E L
authors.initials: EL
authors.lastname: Bradley
authors.forename: D
authors.initials: D
authors.lastname: Parks
mesh_terms: D000818:Animals; D001794:Blood Pressure; D006499:Hepatic Artery / Q000502:physiology; D007035:Hypothermia / Q000503:physiopathology*; D007511:Ischemia / Q000503:physiopathology*; D008099:Liver / Q000503:physiopathology*; D008102:Liver Circulation; D010100:Oxygen / Q000097:blood*; D010101:Oxygen Consumption; D011169:Portal Vein / Q000503:physiopathology; D012039:Regional Blood Flow; D015427:Reperfusion Injury / Q000503:physiopathology*; D013552:Swine; D013556:Swine, Miniature; D014655:Vascular Resistance
chemical_list: D010100:Oxygen