[Components of human complement system. Testing and isolation of C4].
A modified reagent for testing the hemolytic activity of human complement component C4 has been obtained. Reagent R4 was obtained by treatment of human blood serum pools with 0.075 M solution of hydrazine hydrate. This reagent was found to be rich in the serum fraction obtained by chromatography on DEAE-cellulose DE-52 and containing an active complement C3 component. To test the sensitivity and specificity of the reagent, component C4 was subjected to purification. This procedure resulted in a hemolytically active, electrophoretically and immunoelectrophoretically homogeneous component C4. DEAE-cellulose DE-52, DEAE-Sephacel, Ultragel AcA-34 and, again, DEAE-Sephacel were used consecutively as purification agents. The activity yield of component C4 with regard to the initial serum level was 20%.
Chikh
V P
VP
Kosiak
A V
AV
Shukun
S A
SA
Surovtsev
V I
VI
D002850:Chromatography, Gel; D003181:Complement C4 / Q000276:immunology / Q000302:isolation & purification*; D015941:Complement Hemolytic Activity Assay; D006801:Humans; D007122:Immunoelectrophoresis; D007202:Indicators and Reagents
D003181:Complement C4; D007202:Indicators and Reagents
Komponenty sistemy komplementa cheloveka. Testirovanie i vydelenie C4.