title: Cholesterol 26-hydroxylase activity of hamster liver mitochondria: isotope ratio analysis using deuterated 26-hydroxycholesterol.
abstract: Deuterated 26-hydroxycholesterol prepared from diosgenin by modifications of existing methods permitted the determination of mitochondrial cholesterol 26-hydroxylase using endogenous cholesterol as the substrate. Enzyme activity in a group of Syrian hamsters was found to be 10.3 +/- 3.7 pmol.min-1.mg protein-1.
authors.affiliation: Division of Hepatic Diseases, New York University Medical Center, NY 10016.
authors.forename: E
authors.initials: E
authors.lastname: Kok
authors.forename: N B
authors.initials: NB
authors.lastname: Javitt
mesh_terms: D000818:Animals; D053493:Cholestanetriol 26-Monooxygenase; D006224:Cricetinae; D003903:Deuterium; D008647:Mesocricetus; D008930:Mitochondria, Liver / Q000201:enzymology*; D013250:Steroid Hydroxylases / Q000378:metabolism*
chemical_list: D003903:Deuterium; D013250:Steroid Hydroxylases; D053493:Cholestanetriol 26-Monooxygenase