Demonstration of the antiviral role of natural killer cells in vivo with a natural killer cell-specific monoclonal antibody (NK 1.1).
A monoclonal antibody (NK 1.1) to mouse natural killer (NK) cells selectively depleted NK cell activity in virus-infected mice without significantly depressing other immune functions, including the development of virus-specific cytotoxic T cells. NK cell depletion with this antibody resulted in markedly enhanced plaque-forming unit titers of some (murine cytomegalo, Pichinde) but not other (mouse hepatitis, lymphocytic choriomeningitis) viruses. This confirms that NK cells do play a role in regulating certain infections and shows that this antibody provides a convenient tool for examining the role of NK cells in viral infections.
Welsh
R M
RM
Department of Pathology, University of Massachusetts Medical Center, Worcester.
Dundon
P L
PL
Eynon
E E
EE
Brubaker
J O
JO
Koo
G C
GC
O'Donnell
C L
CL
D000818:Animals; D000911:Antibodies, Monoclonal; D003601:Cytotoxicity Tests, Immunologic; D005677:G(M1) Ganglioside; D006028:Glycosphingolipids / Q000276:immunology; D007113:Immunity, Innate / Q000276:immunology / Q000502:physiology; D007694:Killer Cells, Natural / Q000276:immunology / Q000502:physiology*; D008099:Liver / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000382:microbiology; D010948:Viral Plaque Assay; D014777:Virus Diseases / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006028:Glycosphingolipids; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside