title: Clinical studies on bladder tumor-associated antigen in serum of patients with bladder cancer by using monoclonal antibody and sandwich ELISA.
abstract: Investigations on tumor-associated antigen in the serum of patients with bladder cancer by using monoclonal antibody Hb 7A and sandwich ELISA were carried out on 36 patients with bladder cancer (BCa group), 18 patients with other tissue tumor (OTT group) and 22 normal subjects (control group). The average OD value of Hb 7A antigen of BCa group, OTT group and control group was 0.315 +/- 0.033, 0.124 +/- 0.026 and 0.12 +/- 0.021 respectively. The OD value of Hb 7A antigen in BCa group was significantly higher than that of control group and of OTT group (P less than 0.01), but there was no obvious difference between the control group and OTT group (P greater than 0.05). The positive detection rate in BCa group was 86% (31/36), while detection in all 22 normal subjects and the 18 patients of OTT group yielded negative results. The results indicated that the method of using McAb Hb 7A and sandwich ELISA, characterized by high specificity and sensitivity, is of value for clinical diagnosis and follow-up of patients with bladder cancer and general survey of persons at high risk of bladder cancer.
authors.affiliation: Department of Urology, Tongji Medical University, Wuhan.
authors.forename: S S
authors.initials: SS
authors.lastname: Zhu
authors.forename: Y C
authors.initials: YC
authors.lastname: Zhang
authors.forename: R Z
authors.initials: RZ
authors.lastname: Hu
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Ye
authors.forename: C J
authors.initials: CJ
authors.lastname: Li
authors.forename: Q A
authors.initials: QA
authors.lastname: Lin
mesh_terms: D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis*; D002295:Carcinoma, Transitional Cell / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D001749:Urinary Bladder Neoplasms / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm