title: Role of histidine residues in the binding mechanism of the growth hormone family.
abstract: 1. Reactivity of the hGH histidine residues were studied by reaction with ethoxyformic anhydride. Localization in the molecule of three kinetically distinguishable classes, each including only one residue, was achieved. 2. The first was composed of residue 151, with an apparent velocity constant k = 0.735/min, (similar to that of histidines 19 and 21 in bGH and eGH). The second histidine, 18, with a velocity constant k = 0.135/min, (similar to that of histidine 169 in the above hormones), and a third, histidine 21, which does not react at all. 3. Neither histidine 151 nor 18 seem to be involved, at least not directly, in bGH binding to specific rat liver sites, since the decrease in this capacity was only 47% after modification of the former by 77 and 65% after total modification of the latter. 4. These results, and those previously obtained with bGH and eGH, suggest that either histidine 21 is the only indispensable histidine for the binding of growth hormones to specific rat liver sites, or that histidine 21 and/or 18 (19 in bGH and eGH), are located within the growth hormone binding site interaction area.
authors.affiliation: Instituto de Química y Fisicoquímica Biológicas, (UBA-CONICET), Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.
authors.forename: J G
authors.initials: JG
authors.lastname: Fukushima
authors.forename: M J
authors.initials: MJ
authors.lastname: Biscoglio de Jimenez Bonino
authors.forename: O
authors.initials: O
authors.lastname: Cascone
authors.forename: J A
authors.initials: JA
authors.lastname: Santomé
mesh_terms: D000818:Animals; D001665:Binding Sites; D004047:Diethyl Pyrocarbonate; D013006:Growth Hormone / Q000378:metabolism*; D006639:Histidine / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008968:Molecular Conformation; D051381:Rats
chemical_list: D006639:Histidine; D013006:Growth Hormone; D004047:Diethyl Pyrocarbonate