High-performance liquid chromatography of sulphadimethoxine and its N1-glucuronide, N4-acetyl and N4-acetyl-N1-glucuronide metabolites in human plasma and urine.
Sulphadimethoxine is metabolized in humans by N1-glucuronidation and by N4-acetylation. Sulphadimethoxine-N1-glucuronide can be measured by the direct high-performance liquid chromatographic analysis and without enzymic deglucuronidation. The N1-glucuronide can be measured by an isocratic as well as by a gradient mobile phase. The group contribution of the N1-glucuronide moiety to the capacity factor is a reduction of 0.24 in the isocratic system and 0.55 in the gradient system. N4-Acetylation increases the capacity factor by a factor 1.4 in the isocratic system and by 1.06 in the gradient system.
Vree
T B
TB
Department of Clinical Pharmacy, Sint Radboud University Hospital, Nijmegen, The Netherlands.
Kolmer
E W
EW
Martea
M
M
Bosch
R
R
Shimoda
M
M
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005965:Glucuronates / Q000097:blood* / Q000378:metabolism / Q000652:urine; D006801:Humans; D013412:Sulfadimethoxine / Q000097:blood* / Q000378:metabolism / Q000652:urine
D005965:Glucuronates; D013412:Sulfadimethoxine