The cellular myb oncogene is amplified, rearranged and activated in human glioblastoma cell lines.
The human c-myb gene which encodes a DNA binding protein and which is rarely amplified in neoplastic cells was found to be altered in four human glioblastoma cell lines. It exists in multiple copies in 2 out of 4 cases studied. The degree of amplification as determined by densitometry was about 10-fold, a rearrangement within the coding region and an enhanced gene activity of c-myb were noted. The observation of c-myb oncogene amplification and activity in glioblastoma cell lines presents the first report of this effect in human brain tumor cells.
Welter
C
C
Institute of Human Genetics, Medical School, University of the Saar, Homburg/Saar, F.R.G.
Henn
W
W
Theisinger
B
B
Fischer
H
H
Zang
K D
KD
Blin
N
N
D001932:Brain Neoplasms / Q000235:genetics*; D002871:Chromosome Banding; D004273:DNA, Neoplasm / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000235:genetics; D005784:Gene Amplification; D005786:Gene Expression Regulation; D015321:Gene Rearrangement; D005910:Glioma / Q000235:genetics*; D006801:Humans; D008297:Male; D009857:Oncogenes; D012334:RNA, Neoplasm / Q000302:isolation & purification; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; D012334:RNA, Neoplasm