title: Characterization of Nippostrongylus brasiliensis infection in different strains of mice.
abstract: Five mouse strains, CBA/J, BALB/c, C3H/HeJ, A/J, and C57Bl/6J-bg-bg, all showed similar expulsion kinetics for Nippostrongylus brasiliensis (infective dose = 500 L3). Typically, parasite recovery was maximal on day 2 in the lungs and by day 4 in the small intestine. Few worms (less than 5% infective dose) were recovered on day 14 in all strains. These same mouse strains exhibited immune depression on day 5 of infection with mesenteric lymph node cells (MLN) showing reduced (10-30% normal) IgM, IgG, and IgA responses against heterologous antigen. The intestinal mast cell numbers and tissue histamine levels were examined in CBA/J mice. Mast cell numbers increased (normal = less than 1/villous crypt unit; VCU) from day 5 and peaked on day 12 (greater than 15/VCU). Intestinal histamine levels did not completely correlate with mast cell numbers with maximum concentrations (240 +/- 73 ng/g, 2-fold over normal) reached by day 8. Histamine concentrations in the intestine returned to normal levels by day 20.
authors.affiliation: Department of Pathology, McMaster University Medical Center, Hamilton, Ontario, Canada.
authors.forename: A W
authors.initials: AW
authors.lastname: Stadnyk
authors.forename: P J
authors.initials: PJ
authors.lastname: McElroy
authors.forename: J
authors.initials: J
authors.lastname: Gauldie
authors.forename: A D
authors.initials: AD
authors.lastname: Befus
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D002452:Cell Count; D005260:Female; D006632:Histamine / Q000032:analysis; D007136:Immunoglobulins / Q000096:biosynthesis; D007421:Intestine, Small / Q000276:immunology / Q000469:parasitology / Q000473:pathology; D007700:Kinetics; D008168:Lung / Q000469:parasitology; D008297:Male; D008407:Mast Cells / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D009349:Nematode Infections / Q000276:immunology* / Q000469:parasitology; D009559:Nippostrongylus / Q000276:immunology / Q000502:physiology
chemical_list: D000909:Antibodies, Helminth; D007136:Immunoglobulins; D006632:Histamine