Comparative study of automated versus manual extraction of DNA from clinical specimens.
The authors have compared an automated method for DNA extraction with an established manual method using routine clinical specimens. The purity, yield, and quality of the extracted DNA was assessed by ultraviolet absorbency measurements, gel electrophoresis, and DNA probe hybridization. The automated DNA extractor can provide high molecular weight DNA equivalent in purity, yield, and quality to that obtained by the manual method with substantial savings in technologist time and overall costs associated with the procedure.
Taylor
S A
SA
Department of Pathology, Cancer Control Agency of British Columbia, British Columbia Children's Hospital, Vancouver, Canada.
Tsang
A O
AO
Fu
K H
KH
Kalousek
D K
DK
Horsman
D E
DE
D015139:Blotting, Southern; D003365:Costs and Cost Analysis; D004247:DNA / Q000302:isolation & purification*; D015342:DNA Probes; D006801:Humans; D009693:Nucleic Acid Hybridization
D015342:DNA Probes; D004247:DNA