Apoptosis of vascular endothelial cells by fibroblast growth factor deprivation.
Survival and proliferation of many types of vascular endothelial cells are influenced by fibroblast growth factor (FGF)1. Removal of FGF from the medium of human umbilical vein endothelial cells (HUVEC) in culture resulted in death of the cells. Here we show that the death caused by deprivation of FGF is active death or apoptosis, and the process of apoptosis can be inhibited by cycloheximide, an inhibitor of protein synthesis. The present study shows apoptosis occurs in endothelial cells in culture. The process of active death of vascular endothelial cells is inhibited by growth factor. This mechanism may be important for the regulation of vascular organization through the degeneration of vessels.
Araki
S
S
Department of Molecular Biology, School of Science, Nagoya University, Japan.
Shimada
Y
Y
Kaji
K
K
Hayashi
H
H
D000818:Animals; D002417:Cattle; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D003513:Cycloheximide; D004247:DNA / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004730:Endothelium, Vascular / Q000166:cytology*; D005260:Female; D005346:Fibroblast Growth Factors / Q000502:physiology*; D006801:Humans; D014470:Umbilical Cord / Q000098:blood supply
D005346:Fibroblast Growth Factors; D004247:DNA; D003513:Cycloheximide