title: Fluorescence polarization immunoassay for cyclosporine A determination in whole blood.
abstract: A fluorescence polarization immunoassay (FPIA) for cyclosporine A (CsA) determination in blood was evaluated. The method demonstrated a good reproducibility both in within-run and between-run assays. The linearity range was 35-990 micrograms/L. Comparison of measurements in patients' whole blood with radioimmunoassay results showed higher estimates by the FPIA despite the good correlation coefficient. Finally, a poor agreement was observed between the evaluated method and the high-performance liquid chromatographic technique for CsA determination.
authors.affiliation: Department of Clinical Biochemistry, University of Padova, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Plebani
authors.forename: C D
authors.initials: CD
authors.lastname: Paleari
authors.forename: M
authors.initials: M
authors.lastname: Masiero
authors.forename: D
authors.initials: D
authors.lastname: Faggian
authors.forename: A
authors.initials: A
authors.lastname: Burlina
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002851:Chromatography, High Pressure Liquid; D003524:Cyclosporins / Q000097:blood*; D005069:Evaluation Studies as Topic; D005454:Fluorescence Polarization / Q000379:methods*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008875:Middle Aged; D011863:Radioimmunoassay; D015203:Reproducibility of Results
chemical_list: D003524:Cyclosporins