L-pipecolic acid metabolism in human liver: detection of L-pipecolate oxidase and identification of its reaction product.
L-Pipecolate oxidase, an enzyme that oxidizes L-pipecolic acid in the human liver has been demonstrated in the peroxisomal preparation. This enzyme oxidizes L-pipecolic acid with concomitant production of H2O2 in the peroxisome of the normal human liver. The immediate product of L-pipecolic acid oxidation has been identified as L-alpha-aminoadipate delta-semialdehyde. This reaction product was directly, and also after conversion to pipecolic acid by NaBH4 reduction, characterized by use of an amino acid analyzer and thin-layer chromatography. The pit fall of an indirect assay of L-pipecolate oxidase by means of the assay of alpha-aminoadipic acid formation was discussed.
Rao
V V
VV
Department of Biochemistry, University of Maryland Dental School, Baltimore 21201.
Chang
Y F
YF
D015074:2-Aminoadipic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D000328:Adult; D000596:Amino Acids / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D002855:Chromatography, Thin Layer; D006801:Humans; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008830:Microbodies / Q000201:enzymology / Q000378:metabolism*; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000032:analysis*; D010875:Pipecolic Acids / Q000378:metabolism*
D000596:Amino Acids; D015415:Biomarkers; D010875:Pipecolic Acids; D015074:2-Aminoadipic Acid; C000061:allysine; D000587:Oxidoreductases Acting on CH-NH Group Donors; C032771:L-pipecolate dehydrogenase; C031345:pipecolic acid