title: Production of alpha 1-proteinase inhibitor (human).
abstract: A method for large scale isolation of alpha 1-proteinase inhibitor (alpha 1-PI) is described. This method employs waste Cohn Fraction IV-1 as the starting material and involves fractional precipitation with polyethylene glycol followed by ion exchange chromatography on diethylaminoethanol (DEAE)-Sepharose. The process also incorporates a ten hour, at 60 degrees C, heat-treatment step to reduce or eliminate the risk of transmission of viral disease. The final product, having a purity of approximately 60%, is freeze-dried. This preparation behaves almost identically to the alpha 1-PI in plasma and is suitable for replacement therapy in hereditary emphysema.
authors.affiliation: Plasma Manufacturing Technology, Cutter Biological, Miles Inc., Berkeley, California.
authors.forename: R H
authors.initials: RH
authors.lastname: Hein
authors.forename: S M
authors.initials: SM
authors.lastname: Van Beveren
authors.forename: M A
authors.initials: MA
authors.lastname: Shearer
authors.forename: M H
authors.initials: MH
authors.lastname: Coan
authors.forename: W J
authors.initials: WJ
authors.lastname: Brockway
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004588:Electrophoresis, Cellulose Acetate; D006801:Humans; D008722:Methods; D000515:alpha 1-Antitrypsin / Q000032:analysis / Q000302:isolation & purification*
chemical_list: D000515:alpha 1-Antitrypsin