Cytotoxic component(s) of Klebsiella oxytoca on HEp-2 cells.
A cytotoxic component(s) was detected in culture filtrates of Klebsiella oxytoca isolated from patients with antibiotic-associated hemorrhagic colitis. Eleven of 12 isolates exhibited cytotoxicity on HEp-2 cells. The cytotoxic activity of K. oxytoca strain MH43-1 was stable for the treatment of 60 C for 30 min, but inactivated by the treatment of 100 C for 15 min. This cytotoxicity was not destroyed by the treatment with trypsin or pronase, and the component was filtrable through a membrane filter which cut off molecular weight 5,000.
Higaki
M
M
Department of Microbiology, Tokyo Medical and Dental University.
Chida
T
T
Takano
H
H
Nakaya
R
R
D002478:Cells, Cultured; D003092:Colitis / Q000175:diagnosis / Q000382:microbiology*; D003603:Cytotoxins / Q000032:analysis*; D006358:Hot Temperature; D006801:Humans; D007709:Klebsiella / Q000032:analysis / Q000187:drug effects / Q000472:pathogenicity*; D011402:Pronase / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology; D014774:Virulence
D003603:Cytotoxins; D014357:Trypsin; D011402:Pronase