Electric field mediated transfer of enzymes into human oocytes.
In an attempt to develop a simple, atraumatic technique of introducing macromolecules and enzymes into human oocytes, we have investigated the use of electric field mediated transfer. Eighty-seven percent of human oocytes showed cytochemical evidence for the transfer of the enzyme horseradish peroxidase (HRP). The cell viability after this procedure was 89% as determined by the vital dye rhodamine-123. This study demonstrates that the enzyme HRP can be transferred into human oocytes using the electric field mediated method. Functional activity of the enzyme was maintained after this transfer.
Douglas
J W
JW
Ohio State University College of Medicine, Columbus.
Kim
M H
MH
Batten
B E
BE
D015100:3,3'-Diaminobenzidine / Q000008:administration & dosage; D004560:Electricity; D004798:Enzymes / Q000008:administration & dosage*; D005260:Female; D006735:Horseradish Peroxidase / Q000008:administration & dosage; D006801:Humans; D006861:Hydrogen Peroxide / Q000008:administration & dosage; D066298:In Vitro Techniques; D009865:Oocytes / Q000201:enzymology*
D004798:Enzymes; D015100:3,3'-Diaminobenzidine; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase