[Products of expression of cloned phage T4 basal plate genes 29, 28, 27, 51 and 26].
We used bacterial strains with two recombinant plasmids carrying BglII DNA fragment with T4 genes 25-29 in different orientations. In the case of the plasmid pRL705 phage DNA fragment had correct orientation for transcription of T4 late genes, and in the plasmid pRL707 it has the opposite one. Transcription from the PL promoter of the plasmid pRL705 led to the synthesis of 4 proteins which according to the molecular weights observed were the products of T4 genes 29, 28, 27, and 51. In the case of the plasmid pRL707 the synthesis of gene 26 protein with the molecular weight of 24-25 kDa was observed in agreement with the direction of this gene transcription determined earlier. The molecular weight we have obtained appeared to be significantly different from the 40-41 kDa protein identified earlier as gene 26 product. We have determined that among the proteins induced in this system the product of gene 29 only turned out to be unstable and degrading during the prolonged incubation.
Klausa
V I
VI
Nivinskas
R G
RG
D003001:Cloning, Molecular; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D010957:Plasmids; D011401:Promoter Regions, Genetic; D013604:T-Phages / Q000235:genetics*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics*
D014764:Viral Proteins
Produkty ékspressii klonirovannykh genov 29, 28, 27, 51 i 26 bazal'noĭ plastinki bakteriofaga T4.