title: The gene for the ubiquitous octamer-binding protein Oct-1 is on human chromosome 1, region cen-q32, and near Ly-22 and Ltw-4 on mouse chromosome 1.
abstract: Oct-1 is a sequence-specific transcription and DNA replication factor that recognizes the octameric sequence ATGCAAAT. This protein shares an extended region of sequence similarity, called the POU domain, with the lymphoid-specific transcription factor Oct-2, the pituitary-specific transcription factor Pit-1, and the Caenorhabditis elegans cell lineage gene product unc-86. Two subdomains, POU-related homeobox and POU-specific box, lie within the POU domain. Unlike other POU or homeodomain proteins, Oct-1 is ubiquitously expressed. A ubiquitous 95- to 100-kDa protein that has the same DNA-binding properties and is variously referred to as OTF-1, NFIII, or OBP100 is probably identical to Oct-1. The human gene was mapped by Southern blot analyses of human x rodent hybrid cell lines to chromosome 1, region cen-q32. In Chinese hamster x mouse hybrid cell lines the mouse locus was also mapped to chromosome 1. With the BXD (from progenitor strains C57BL/6J and DBA/2J) set of recombinant inbred strains of mice, the murine gene was localized on the linkage map of mouse chromosome 1. There were no recombinants among 26 strains with Ly-22 and 1 recombinant among 24 strains with Ltw-4. The gene symbol is OTF1 for humans and Oct-1 for mouse.
authors.affiliation: Howard Hughes Medical Institute, Stanford, California.
authors.forename: C L
authors.initials: CL
authors.lastname: Hsieh
authors.forename: R
authors.initials: R
authors.lastname: Sturm
authors.forename: W
authors.initials: W
authors.lastname: Herr
authors.forename: U
authors.initials: U
authors.lastname: Francke
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000235:genetics; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D006224:Cricetinae; D003412:Cricetulus; D004268:DNA-Binding Proteins / Q000235:genetics*; D005796:Genes; D008040:Genetic Linkage; D051863:Host Cell Factor C1; D006801:Humans; D006822:Hybrid Cells; D051379:Mice / Q000235:genetics*; D008815:Mice, Inbred Strains / Q000235:genetics; D005810:Multigene Family; D050811:Octamer Transcription Factor-1; D010802:Phylogeny; D051381:Rats; D013045:Species Specificity; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D000950:Antigens, Ly; D004268:DNA-Binding Proteins; C497692:HCFC1 protein, human; C497693:Hcfc1 protein, mouse; D051863:Host Cell Factor C1; D050811:Octamer Transcription Factor-1; C494165:POU2F1 protein, human; C494166:Pou2f1 protein, mouse; C494168:Pou2f1 protein, rat; D014157:Transcription Factors