Treatment of antibodies to reduce non-specific binding in immunoassays using the avidin-biotin complex.
Three different methods to reduce non-specific binding of antibodies to avidin coated plates in an immunoassay are described and compared: The use of a blocking agent, antiserum adsorption on avidin-agarose and antiserum adsorption to avidin by a so called pre-incubation method. The pre-incubation method proved to be highly efficient, fast and simple. Further applications of this method to different immunoassay systems are proposed in the discussion section.
Bodmer
D M
DM
Medical Bioanalytics Project, Paul Scherrer Institute, Villigen, Switzerland.
Tiefenauer
L X
LX
D000327:Adsorption; D000818:Animals; D000918:Antibody Specificity; D001360:Avidin / Q000276:immunology; D001710:Biotin / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004958:Estradiol / Q000276:immunology / Q000378:metabolism*; D008892:Milk / Q000276:immunology; D011817:Rabbits
D001360:Avidin; D004958:Estradiol; D001710:Biotin