Islet amyloid polypeptide, a novel pancreatic peptide, is a circulating hormone secreted under glucose stimulation.
Islet amyloid polypeptide (IAPP) was identified in human plasma using immunoaffinity chromatography, gel filtration and reverse- phase high performance liquid chromatography coupled with radioimmunoassay specific for the peptide. IAPP[1-37], IAPP[17-37], and other two IAPP- related peptides which were putative pro-IAPPs or different processing products of IAPP, were isolated. All of these IAPPs were also found in human pancreatic extract, indicating that they were secreted from B cell secretory granules into the circulation. IAPP[1-37] is a major molecular form of IAPP in the pancreas, but it accounted for 31% of immunoreactive IAPP in the plasma. Plasma concentration of IAPP in normal individuals increased to 3.0 times the basal level in response to oral administration of 75 g glucose. This study indicated that IAPP is a circulating hormone secreted under glucose stimulation.
Nakazato
M
M
Third Department of Internal Medicine, Miyazaki Medical College, Japan.
Miyazato
M
M
Asai
J
J
Mitsukawa
T
T
Kangawa
K
K
Matsuo
H
H
Matsukura
S
S
D000328:Adult; D000682:Amyloid / Q000032:analysis / Q000097:blood* / Q000378:metabolism; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005215:Fasting; D005947:Glucose; D006801:Humans; D007074:Immunoglobulin G; D058228:Islet Amyloid Polypeptide; D007700:Kinetics; D008297:Male; D010179:Pancreas / Q000032:analysis / Q000473:pathology; D011863:Radioimmunoassay; D012016:Reference Values
D000682:Amyloid; D007074:Immunoglobulin G; D058228:Islet Amyloid Polypeptide; D005947:Glucose