The binding of cadmium to crab cadmium metallothionein. A polarographic investigation.
Differential pulse polarography was used to monitor the titration of crab (Cancer pagurus) cadmium metallothionein with additional cadmium. Scatchard analysis was used to calculate an apparent second-order dissociation constant. A mean value of 3.60 x 10(-7) M was calculated at 20 degrees C in 0.016 M-NaClO4, pH 8.0.
Sparla
A M
AM
Dunstaffnage Marine Laboratory, Scotland, U.K.
Overnell
J
J
D000818:Animals; D003386:Brachyura / Q000378:metabolism*; D002104:Cadmium / Q000378:metabolism*; D007700:Kinetics; D008668:Metallothionein / Q000378:metabolism*; D011048:Polarography / Q000379:methods; D011485:Protein Binding
D002104:Cadmium; D008668:Metallothionein