[Comparative evaluation of the glycosaminoglycan composition in plasma membranes of normal and transformed mouse liver cells].
Using electrophoresis in agarose gel, a comparative study of composition of membrane-bound glycosaminoglycans (GAG) from normal mouse liver cells and from o-aminoazatoluene-induced mouse hepatoma cells was carried out. Differences in the composition and localization of GAG were revealed. The plasma membrane fraction of hepatoma cells contained no GAG; the bulk of GAG (approximately 98%) was localized in the plasma membrane free fraction. Within this fraction GAG contained no heparan sulfates with a high electrophoretic mobility that were detected in plasma membranes of normal liver cells. The possible involvement of proteoglycans bound to cell surface in transmembrane signal transfer is discussed.
Rykova
V I
VI
Kropotova
S A
SA
Kaledin
V I
VI
Semenova
L A
LA
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006025:Glycosaminoglycans / Q000302:isolation & purification / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D009762:o-Aminoazotoluene
D006025:Glycosaminoglycans; D009762:o-Aminoazotoluene
Sravnitel'noe issledovanie sostava glikozaminoglikanov v plazmaticheskikh membranakh normal'nykh i transformirovannykh kletok pecheni mysheĭ.