Rapid analysis and purification of polymerase chain reaction products by high-performance liquid chromatography.
This report describes the use of high-performance liquid chromatography (HPLC) for the rapid analysis and purification of the polymerase chain reaction products. Employing a new anion-exchange nonporous column, efficient separations of both DNA restriction fragments and amplified PCR products are achieved in 10 to 20 minutes and quantitated within +/- 10%. The performance of the HPLC technique is described in terms of resolution, reproductibility, sensitivity and micropreparative capability and compared to that of gel electrophoresis for this application.
Katz
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ED
Perkin-Elmer Corporation, Norwalk, CT 06859.
Dong
M W
MW
D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D005784:Gene Amplification; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results
D004247:DNA