Potent inflammatory properties in human skin of interleukin-1 alpha-like material isolated from normal skin.
Recombinant human interleukin 1 (IL-1) possesses potent inflammatory properties in both animal and human skin. However, IL-1-like material has been isolated from normal epidermal samples. In view of the uncertainty concerning the structure and biologic properties of human epidermal IL-1, heel stratum corneum, and chamber fluid samples from normal skin have been purified by successive reversed phase and anion exchange high-performance liquid chromatography (HPLC), and aliquots of each HPLC fraction tested for IL-1 activity in an EL-4 NOB-1 assay and for inflammatory activity by intradermal injection of autologous material. The results consistently indicated the presence of inflammatory quantities of IL-1 alpha-like material, which induced persistent erythema lasting at least 24 h, associated in 4-h biopsies with mixed dermal leukocyte infiltrates containing increased numbers of neutrophils, monocytes, and T helper cells. Biologically active quantities of IL-1 beta were not recovered following HPLC purification in most experiments. Analysis of heel stratum corneum extract also showed that the majority of the dilution-related IL-1 activity detected in the EL-4 NOB-1 bioassay was neutralized by IL-1 alpha but not IL-1 beta antiserum. These findings suggest that IL-1 alpha-like material is present in inflammatory amounts in normal human epidermis, and its release may represent a primary inflammatory mechanism in human skin.
Camp
R
R
Institute of Dermatology, St. Thomas's Hospital, London, U.K.
Fincham
N
N
Ross
J
J
Bird
C
C
Gearing
A
A
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D003872:Dermatitis / Q000503:physiopathology*; D005260:Female; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007375:Interleukin-1 / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D007962:Leukocytes / Q000473:pathology; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D012867:Skin / Q000378:metabolism; D012879:Skin Physiological Phenomena
D007106:Immune Sera; D007375:Interleukin-1