Affinities of SM-7338 for penicillin-binding proteins and its release from these proteins in Staphylococcus aureus.
SM-7338, a carbapenem antibiotic, had high affinities for penicillin-binding proteins (PBPs) 1, 2, and 4 of Staphylococcus aureus but not for PBP 3 when a competition assay with [14C]benzylpenicillin was used. However, binding of [14C]SM-7338 was saturated for PBP 3 at a concentration of 1 microgram/ml. These results were due to the rapid release of SM-7338 from PBP 3-SM-7338 complexes with a half-life of 2 min.
Sumita
Y
Y
Research Laboratories, Sumitomo Pharmaceuticals Co., Ltd., Osaka, Japan.
Fukasawa
M
M
Okuda
T
T
D001426:Bacterial Proteins / Q000378:metabolism; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000378:metabolism*; D006602:Hexosyltransferases; D000077731:Meropenem; D002267:Muramoylpentapeptide Carboxypeptidase / Q000378:metabolism*; D046915:Penicillin-Binding Proteins; D010458:Peptidyl Transferases; D011485:Protein Binding; D013211:Staphylococcus aureus / Q000378:metabolism*; D013845:Thienamycins / Q000378:metabolism*
D001426:Bacterial Proteins; D002352:Carrier Proteins; D046915:Penicillin-Binding Proteins; D013845:Thienamycins; D010458:Peptidyl Transferases; D006602:Hexosyltransferases; D002267:Muramoylpentapeptide Carboxypeptidase; D000077731:Meropenem