title: Gova/b alloantigen system on human platelets.
abstract: In this report we describe a platelet alloantigen system that is carried on a novel platelet protein of 175 Kd. Antisera against the two alleles (Gova/Govb) were found in two patients who had received large numbers of platelet transfusions. The anti-Gov alloantibodies could not be detected using a whole platelet solid phase enzyme immunoassay, or by a platelet glycoprotein capture enzyme immunoassay using monoclonal antibodies against glycoproteins Ib/IX, Ia/IIa, and IIb/IIIa. Using radioimmunoprecipitation techniques, a protein was precipitated that migrated at 175 Kd (reduced). Under nonreduced conditions, a 150-Kd protein was detected with a minor component at 175Kd. The detection of the alloantigens was not activation-dependent. Using immunodepletion studies, we demonstrated that each alloantiserum recognized an epitope on a discrete population of the 175-Kd platelet protein. Family studies demonstrated that the alloantigens designated as Gova and Govb were inherited in an autosomal codominant fashion. The phenotypic frequencies were Gova/Gova, 26%; Gova/Govb, 55%; Govb/Govb, 19%; giving gene frequencies of 0.532 and 0.468 for Gova and Govb, respectively (n = 33).
authors.affiliation: Department of Medicine, McMaster Medical Centre, Hamilton, Ontario, Canada.
authors.forename: J G
authors.initials: JG
authors.lastname: Kelton
authors.forename: J W
authors.initials: JW
authors.lastname: Smith
authors.forename: P
authors.initials: P
authors.lastname: Horsewood
authors.forename: J R
authors.initials: JR
authors.lastname: Humbert
authors.forename: C P
authors.initials: CP
authors.lastname: Hayward
authors.forename: T E
authors.initials: TE
authors.lastname: Warkentin
mesh_terms: D000483:Alleles; D001792:Blood Platelets / Q000276:immunology*; D005260:Female; D006801:Humans; D039421:Integrin alpha2; D007519:Isoantigens / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000032:analysis / Q000276:immunology; D010375:Pedigree; D010980:Platelet Membrane Glycoproteins / Q000032:analysis / Q000276:immunology; D011233:Precipitin Tests
chemical_list: C056564:ITGA2B protein, human; D039421:Integrin alpha2; D007519:Isoantigens; D008562:Membrane Glycoproteins; D010980:Platelet Membrane Glycoproteins