Mouse bone marrow cytogenetic damage produced by residues of tequila.
Five concentrations (50-860 mg/kg) of residues obtained after distillation and lyophilization of commercial tequila were injected into mice for evaluation of chromosome aberrations, sister-chromatid exchanges, and proliferation kinetics in mouse bone marrow cells. Appropriate positive and negative controls were included. Our results showed significant dose-related increases of chromosomal aberrations starting at 50 mg/kg and for sister-chromatid exchanges at 430 mg/kg. Cellular proliferation kinetics showed no alterations. With these data we demonstrated that the residues of tequila are genotoxic in vivo.
Madrigal-Bujaidar
E
E
Depto. de Morfología, Escuela Nacional de Ciencias Biológicas, I.P.N. Carpio y Plan de Ayala, Mexico City, Mexico.
Rojas
A
A
Ramos
A
A
Rosas
E
E
Díaz Barriga-Arceo
S
S
D000434:Alcoholic Beverages / Q000633:toxicity*; D000818:Animals; D001853:Bone Marrow / Q000648:ultrastructure; D002455:Cell Division / Q000187:drug effects; D002869:Chromosome Aberrations; D008297:Male; D051379:Mice; D012854:Sister Chromatid Exchange