Type II fiber activation with electrical stimulation: a preliminary report.
Electrical stimulation to augment or maintain muscle performance has been well documented. The purpose of this preliminary report is to present the results of a single-case study conducted to determine the order of activation of skeletal muscle fibers as a result of electrical stimulation. The subject's quadriceps femoris muscles were electrically stimulated at 80% of maximal isometric torque. Pre-stimulation and immediate post-stimulation muscle biopsy samples were obtained, and a modification of the glucogen-depletion method was used to determine activation of muscle fibers. The pre-stimulation muscle biopsy sample demonstrated uniform periodic acid-Schiff (PAS)-positive staining in all fiber types, whereas the post-stimulation muscle biopsy sample showed glycogen depletion of type II muscle fibers. The most PAS-negative muscle fibers were type IIa skeletal muscle fibers. The results of this single-case study provide evidence that electrical stimulation, as described, selectively activates type II skeletal muscle fibers. The implication of this finding is that, in many chronic diseases, type II fibers are selectively and preferentially affected. Electrical stimulation may be a clinically viable technique to use in patients with type II fiber involvement.
Sinacore
D R
DR
Department of Anatomy, West Virginia University Medical School, Morgantown 26506.
Delitto
A
A
King
D S
DS
Rose
S J
SJ
D000328:Adult; D001706:Biopsy; D004599:Electric Stimulation Therapy / Q000009:adverse effects / Q000592:standards*; D005069:Evaluation Studies as Topic; D006003:Glycogen / Q000032:analysis; D006801:Humans; D008297:Male; D009119:Muscle Contraction; D009132:Muscles / Q000032:analysis / Q000166:cytology / Q000502:physiology*; D009133:Muscular Atrophy / Q000517:prevention & control / Q000628:therapy; D010503:Periodic Acid-Schiff Reaction
D006003:Glycogen