Polypeptide core of a human pancreatic tumor mucin antigen.
This work describes the molecular properties of the polypeptide core of a human pancreatic mucin antigen isolated from a human pancreatic adenocarcinoma cell line, HPAF. Pancreatic tumor mucin was isolated by a combination of molecular sieve chromatography and CsCl/4 M guanidine-HCl density gradient ultracentrifugation. Trifluoromethane sulfonic acid was used to remove carbohydrate units from purified mucin molecules. A rabbit monospecific polyclonal antibody was generated against pancreatic apomucin and reacted with a Mr greater than 200,000 species. The antibody binding data indicated that the rabbit antiserum raised against pancreatic apomucin cross-reacted with a breast mucin synthetic peptide. Northern blot and immunodot blot analyses of various cell line extracts revealed that a tandem repeat sequence and a similar mRNA were detected in both pancreatic and breast mucin-producing cell lines. These results suggest that pancreatic apomucin and breast apomucin share some similarity in tandem repeated nucleic acid and protein sequences.
Lan
M S
MS
Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina 27710.
Hollingsworth
M A
MA
Metzgar
R S
RS
D000230:Adenocarcinoma / Q000276:immunology*; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000235:genetics; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D009077:Mucins / Q000032:analysis* / Q000235:genetics; D009363:Neoplasm Proteins / Q000032:analysis / Q000235:genetics / Q000276:immunology; D015345:Oligonucleotide Probes; D010190:Pancreatic Neoplasms / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D014407:Tumor Cells, Cultured
D000596:Amino Acids; D000951:Antigens, Neoplasm; D009077:Mucins; D009363:Neoplasm Proteins; D015345:Oligonucleotide Probes; D012333:RNA, Messenger