Possible role of rat fatty acid-binding proteins in the intestine as carriers of phenol and phthalate derivatives.
Fatty acid-binding proteins of hepatic and intestinal type and gastrotropin-like protein (GTLP) were purified from rat intestinal cytosol by Sephadex G-75 gel filtration and DEAE-cellulose, CM-cellulose, and hydroxylapatite chromatographies. In addition to fatty acids, butylated hydroxytoluene (BHT), phthalate dibutyl, and di(2-ethylhexyl) esters (DBP and DEHP) were identified by gas liquid chromatography and mass spectrometry as endogenous ligands from the extract of either fatty acid-binding protein superfamily. These protein families in the intestine may have an important role as carriers in the initial step of arresting these exogenous pollutants.
Kanda
T
T
Department of Biochemistry, Niigata University School of Medicine, Japan.
Ono
T
T
Matsubara
Y
Y
Muto
T
T
D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D008401:Gas Chromatography-Mass Spectrometry; D005768:Gastrointestinal Hormones / Q000302:isolation & purification / Q000502:physiology; D007413:Intestinal Mucosa / Q000378:metabolism*; D008024:Ligands; D008099:Liver / Q000378:metabolism; D008297:Male; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D010636:Phenols / Q000378:metabolism*; D010795:Phthalic Acids / Q000378:metabolism*; D051381:Rats
D002352:Carrier Proteins; C490174:Fabp7 protein, rat; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005768:Gastrointestinal Hormones; D008024:Ligands; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D010636:Phenols; D010795:Phthalic Acids; C057275:fatty acid-binding protein 6