Interleukin-2 induces motility of human lymphocytes, but not their mobility.
Interleukin-2 induces cytotoxic and antitumor activities of human lymphocytes. For the expression of these activities, the cytoskeletal system is probably activated. This study was do;ne to find if interleukin-2 causes cell movement. Lymphocytes were incubated with interleukin-2, and their morphology and motile activities were studied. After 72 hr of incubation, some 29% of lymphocytes were larger than before; nucleoli had formed and the microvilli were well-developed. The membrane potential of the lymphocytes increased during incubation. Motility under agar after 3 days of incubation with interleukin-2 was examined. Cells aggregated in clumps in the incubation well, and migration was not observed. When mobility was examined with Boyden's method, fewer cells incubated with interleukin-2 migrated than in control preparations. Cells incubated with interleukin-2 were extracted with Triton X-100. The extract obtained had three more bands on sodium dodecyl sulfate-polyacrylamide gel electrophoresis than the controls. The band were at the positions for the molecular weights of 270,000-300,000. We concluded that interleukin-2 activated the motility of lymphocytes, but not their mobility.
Hiyoshi
M
M
Department of Laboratory Medicine, Osaka City University Medical School, Japan.
Sasaki
A
A
Hashimoto
K
K
Park
K
K
Im
T
T
Tatsumi
N
N
Okuda
K
K
D002449:Cell Aggregation; D002465:Cell Movement; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D008214:Lymphocytes / Q000166:cytology / Q000502:physiology*; D008564:Membrane Potentials; D008970:Molecular Weight; D013997:Time Factors
D007376:Interleukin-2