Immunochemical studies of oligosaccharides obtained from the lipopolysaccharide of Brucella ovis.
Brucella ovis rough lipopolysaccharide (R-LPS) was studied with respect to its heterogeneity, chain length, sugar composition and immunological activity. R-LPS was mildly hydrolysed and oligosaccharides were recovered in the upper phase after partition with chloroform-methanol. Gel-filtration of the upper phase in a column of Bio-Gel P-2 yielded oligosaccharides of 2, 4, 6 and 7 monosaccharide units, 2-keto-deoxy-octulosonic acid (KDO), and monosaccharides. Strong acid hydrolysis followed by paper chromatography showed that the hexa- and heptasaccharides are both composed of glucose, KDO and an unidentified sugar while tetrasaccharide is composed of glucose, mannose and glucosamine. These three oligosaccharides were able to inhibit the LPS-antibody reaction in a solid phase radioimmunoassay, suggesting the oligosaccharides bear antigenic determinants of LPS.
Suarez
C E
CE
División Aplicaciones Agropecuarias, Comisión Nacional de Energía Atómica, Buenos Aires, Argentina.
Pacheco
G A
GA
Vigliocco
A M
AM
D000818:Animals; D002002:Brucella / Q000032:analysis* / Q000276:immunology; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D006868:Hydrolysis; D007150:Immunohistochemistry; D008070:Lipopolysaccharides / Q000032:analysis* / Q000276:immunology; D009844:Oligosaccharides / Q000032:analysis* / Q000276:immunology; D011863:Radioimmunoassay; D012756:Sheep
D008070:Lipopolysaccharides; D009844:Oligosaccharides