Crystallization and preliminary X-ray diffraction studies of a deglycosylated glucose oxidase from Aspergillus niger.
Deglycosylation was shown to be an important prerequisite step for the crystallization of glucose oxidase from Aspergillus niger. Whereas the glycosylated enzyme could not be crystallized, crystals of the deglycosylated enzyme suitable for X-ray diffraction analysis were reproducibly grown in the presence of 1.6 M-ammonium sulphate and octanetriol at pH 5.3 to 5.6. The crystals belong to the space group P3(1)21 or P3(2)21 with refined lattice constants of a = 66.5 A and c = 214.4 A, indicating a cell content of one monomer per asymmetric unit of the crystal. Crystals diffract to at least 2.5 A resolution. Cleavage of 95% of its carbohydrate moiety affected the kinetics of glucose oxidation, stability at low pH and some electrophoretic properties of glucose oxidase, such as molecular mass and the number of isoelectric forms. However, other properties, such as thermal stability, pH and temperature optima of activity were not affected.
Kalisz
H M
HM
Department of Enzyme Technology, GBF-Gesellschaft für Biotechnologische Forschung, Braunschweig, FRG.
Hecht
H J
HJ
Schomburg
D
D
Schmid
R D
RD
D001234:Aspergillus niger / Q000201:enzymology*; D003460:Crystallization; D005949:Glucose Oxidase / Q000302:isolation & purification / Q000378:metabolism; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D014961:X-Ray Diffraction
D005949:Glucose Oxidase