Modulation by polyamines of DNA-dependent DNA polymerase activity from human serum.
1. Spermine, spermidine and putrescine activated DNA-dependent DNA polymerase from human sera by 47-125% at the concentrations of 0.2, 3 and 30 mM, respectively. 2. The polyamines shifted the optimal MgCl2 concentration for the polymerase activity from 10 mM to more physiological 5 mM. 3. Histamine having amino and imino groups at both ends of the molecule also increased the DNA polymerase activity, while cyclopentylamine and n-butylamine showed no effects on the enzyme activity. 4. The stimulatory effect of polyamines on the DNA polymerase activity was more evident with poly(dC)p(dG) used as a template/primer than with poly(dA)p(dT).
Shimamura
S
S
Department of Biochemistry, Mie University School of Medicine, Japan.
Hibasami
H
H
Kano
U
U
Watanabe
S
S
Suzuki
S
S
Nakashima
K
K
D004247:DNA / Q000096:biosynthesis; D004256:DNA Polymerase I / Q000097:blood*; D004789:Enzyme Activation / Q000187:drug effects; D006632:Histamine / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D015636:Magnesium Chloride / Q000494:pharmacology; D011067:Poly dA-dT / Q000378:metabolism; D011073:Polyamines / Q000494:pharmacology*; D011089:Polydeoxyribonucleotides / Q000378:metabolism; D011700:Putrescine / Q000494:pharmacology; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology; D013698:Templates, Genetic
D011073:Polyamines; D011089:Polydeoxyribonucleotides; D015636:Magnesium Chloride; D011067:Poly dA-dT; C019530:poly(dC-dG); D013096:Spermine; D006632:Histamine; D004247:DNA; D004256:DNA Polymerase I; D013095:Spermidine; D011700:Putrescine