Transformation of the rice blast fungus Magnaporthe grisea to hygromycin B resistance.
Low frequency, integrative transformation of three fertile hermaphroditic strains of Magnaporthe grisea has been achieved using plasmid pAN7-1 and cosmid pAN7-2, which contain an Escherichia coli hygromycin B phosphotransferase gene linked to Aspergillus nidulans regulatory sequences.
Leung
H
H
Department of Plant Pathology, University of Wisconsin, Madison 53706.
Lehtinen
U
U
Karjalainen
R
R
Skinner
D
D
Tooley
P
P
Leong
S
S
Ellingboe
A
A
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001203:Ascomycota / Q000187:drug effects / Q000235:genetics*; D015139:Blotting, Southern; D003360:Cosmids; D004352:Drug Resistance, Microbial / Q000235:genetics; D006921:Hygromycin B / Q000494:pharmacology*; D010957:Plasmids; D014170:Transformation, Genetic
D000900:Anti-Bacterial Agents; D006921:Hygromycin B