A stuttering model for paramyxovirus P mRNA editing.
Paramyxovirus P genes are transcribed into two mRNAs which differ from each other by either one (measles and Sendai virus) or two (SV5 and mumps virus) G insertions, and which code for either the P or V proteins. The G insertions always occur within a short run of Gs, and a stuttering mechanism for the insertions has been suggested in which the viral polymerase reiteratively copies a template C residue during mRNA synthesis. Support for this mechanism was obtained by varying the reaction conditions during Sendai virus mRNA synthesis in vitro. A stuttering model is proposed which accounts for how the ratio of inserted to uninserted mRNAs is controlled, and why some paramyxoviruses insert one G and others two Gs when insertions occur.
Vidal
S
S
Department of Microbiology, University of Geneva School of Medicine, Switzerland.
Curran
J
J
Kolakofsky
D
D
D001483:Base Sequence; D006367:HeLa Cells; D007700:Kinetics; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010252:Paramyxoviridae / Q000235:genetics*; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000378:metabolism*
D012333:RNA, Messenger