Inhibition of ubiquinone synthesis in isolated rat heart under an ischemic condition.
1. The biosynthesis of ubiquinone (UQ) in isolated rat heart under ischemic and hypoxic conditions was investigated. 2. Under ischemic perfusion, a greater amount of biosynthetic intermediates, 3-nonaprenyl and 3-decaprenyl-4-hydroxybenzoate (PPHBs) was accumulated and a smaller amount of UQ-9 and -10 was synthesized when compared with normal conditions. 3. The accumulation of PPHBs was observed without forming UQs during anaerobic perfusion. 4. Hydroxylation which is the following reaction of PPHBs for the biosynthesis of UQ in rat heart, was proceeded by the monooxygenase(s) depending upon the oxygen concentrations.
Sugawara
H
H
Department of Pharmacology, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Yamamoto
T
T
Shimizu
S
S
Momose
K
K
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003327:Coronary Disease / Q000378:metabolism*; D006900:Hydroxylation; D000860:Hypoxia / Q000378:metabolism*; D008297:Male; D008929:Mitochondria, Heart / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D010100:Oxygen / Q000494:pharmacology; D010226:Parabens / Q000378:metabolism; D011725:Pyridines / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012402:Rotenone / Q000494:pharmacology; D013729:Terpenes / Q000378:metabolism; D014451:Ubiquinone / Q000378:metabolism*
D010226:Parabens; D011725:Pyridines; D013729:Terpenes; D012402:Rotenone; D014451:Ubiquinone; C013109:metapyrone; C008665:3-nonaprenyl-4-hydroxybenzoate; C064464:3-decaprenyl-4-hydroxybenzoate; C038193:4-hydroxybenzoic acid; D010100:Oxygen