title: Charge-coupled device imaging of rapid calcium transients in cultured arterial smooth muscle cells.
abstract: Transient changes in the concentration of intracellular free calcium are associated with the transduction of primary signals and the subsequent employment of Ca2+ as a second messenger in a multitude of cell types. These transients, typically monitored with the calcium-sensitive fluorescent dye Fura-2, are known to occur with a time course in the order of seconds. In order to accurately monitor such rapid changes in intracellular free calcium concentration in both single cells and simultaneously in several cells in a single field, we have developed a digital fluorescence imaging system based on a charge-coupled device (CCD) camera. We report here on the detailed kinetics of calcium increases in cultured arterial swine smooth muscle cells in response to the agonist ATP.
authors.affiliation: Department of Chemical Engineering, University of Michigan, Ann Arbor.
authors.forename: J J
authors.initials: JJ
authors.lastname: Linderman
authors.forename: L J
authors.initials: LJ
authors.lastname: Harris
authors.forename: L L
authors.initials: LL
authors.lastname: Slakey
authors.forename: D J
authors.initials: DJ
authors.lastname: Gross
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001572:Benzofurans; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D005456:Fluorescent Dyes; D016257:Fura-2; D007700:Kinetics; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D015398:Signal Transduction; D013552:Swine
chemical_list: D001572:Benzofurans; D005456:Fluorescent Dyes; D000255:Adenosine Triphosphate; D002118:Calcium; D016257:Fura-2