Reduction of interleukin-2-receptor expression on retroplacental blood lymphocytes in human pregnancy.
Retroplacental blood lymphocytes (RPL) in human pregnancy were studied for proliferative response induced by recombinant interleukin-2 (rIL-2) and anti-Tac positive cells before and after PHA stimulation for 24 hr and compared with peripheral blood lymphocytes (PBL) of the same donor. Proliferation of RPL induced by IL-2 was significantly lower than that of PBL in all of six cases. In addition, flow cytometry analysis of IL-2 receptors (IL-2R) revealed that IL-2R expression of RPL after stimulation with PHA for 24 hr was significantly less than that of PBL in three of four cases and that RPL showed significantly fewer IL-2R than PBL even at resting state in one case. These results suggest that RPL seem likely to be composed of reduced numbers of lymphocytes bearing IL-2R of non-Tac protein and to have impaired capacity for the acquisition of high-affinity receptors for IL-2.
Yoshida
M
M
Department of Gynecology and Obstetrics, Faculty of Medicine, Kyoto University, Japan.
Kanzaki
H
H
Kariya
M
M
Emi
N
N
Imai
K
K
Okamoto
N
N
Uchida
A
A
Kasakura
S
S
Mori
T
T
D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007376:Interleukin-2 / Q000502:physiology; D008213:Lymphocyte Activation / Q000502:physiology; D008214:Lymphocytes / Q000276:immunology*; D010920:Placenta / Q000276:immunology*; D011247:Pregnancy; D015375:Receptors, Interleukin-2 / Q000378:metabolism*
D007376:Interleukin-2; D015375:Receptors, Interleukin-2