title: Effect of hypophysectomy, replacement therapy with ovine prolactin, and cortisol and triiodothyronine treatment on prolactin receptors of the tilapia (Oreochromis mossambicus).
abstract: The effects of hypophysectomy and subsequent replacement therapy with ovine prolactin (oPRL) on specific binding of 125I-oPRL to gill, kidney, and liver membranes of male tilapia were examined. The possible control of prolactin receptors by cortisol (F) and triiodothyronine (T3) was also studied using intact animals. In gill and kidney, hypophysectomy resulted in a significant decrease in specific binding that was partially restored (threefold increase) by three injections of oPRL, suggesting a role of the pituitary in the control of prolactin receptors. However, removal of the pituitary and replacement therapy with oPRL had no effect on binding by liver membranes. Cortisol and T3 treatment, alone or in combination, did not significantly affect prolactin binding by any of the tissues tested.
authors.affiliation: Department of Integrative Biology, University of California, Berkeley 94720.
authors.forename: S
authors.initials: S
authors.lastname: Dauder
authors.forename: G
authors.initials: G
authors.lastname: Young
authors.forename: H A
authors.initials: HA
authors.lastname: Bern
mesh_terms: D000818:Animals; D004343:Drug Implants; D005399:Fishes / Q000378:metabolism*; D005880:Gills / Q000378:metabolism; D006854:Hydrocortisone / Q000097:blood / Q000494:pharmacology*; D007016:Hypophysectomy; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D010902:Pituitary Gland / Q000502:physiology*; D011388:Prolactin / Q000378:metabolism / Q000494:pharmacology*; D011485:Protein Binding; D011981:Receptors, Prolactin / Q000187:drug effects* / Q000378:metabolism; D012756:Sheep; D014284:Triiodothyronine / Q000097:blood / Q000494:pharmacology*
chemical_list: D004343:Drug Implants; D011981:Receptors, Prolactin; D014284:Triiodothyronine; D011388:Prolactin; D006854:Hydrocortisone