title: Acetobacter cellulose pellicle as a temporary skin substitute.
abstract: A bacterial strain with morphological and biochemical properties close to Acetobacter xylinum has been cultured in nonagitated, inverted sucrose- and yeast water-based medium for the production of thick, smooth, and floating cellulosic pellicles. The cellulose content (greater than 90%, dry weight, depending on the efficiency of water washing) and the beta-D-homopolyglucan nature of these pellicles were assessed by physical, chemical, and enzymatic methods. The apyrogenic bacterial biomass, a minor component of the dried biofilm (BioFill), is inactivated by ethylene dioxide. Once applied on exudating or bloody tissues, this biofilm displays several advantages as a biological dressing, and hence, it is valuable as a temporary skin substitute in the treatment of skin wounds, such as burns, ulcers, grafts, and as an adjuvant in dermal abrasions.
authors.affiliation: LQBB-Biomass Chemio/Biotechnology Lab, Department of Biochemistry, UFPR, Curitiba-PR, Brazil.
authors.forename: J D
authors.initials: JD
authors.lastname: Fontana
authors.forename: A M
authors.initials: AM
authors.lastname: de Souza
authors.forename: C K
authors.initials: CK
authors.lastname: Fontana
authors.forename: I L
authors.initials: IL
authors.lastname: Torriani
authors.forename: J C
authors.initials: JC
authors.lastname: Moreschi
authors.forename: B J
authors.initials: BJ
authors.lastname: Gallotti
authors.forename: S J
authors.initials: SJ
authors.lastname: de Souza
authors.forename: G P
authors.initials: GP
authors.lastname: Narcisco
authors.forename: J A
authors.initials: JA
authors.lastname: Bichara
authors.forename: L F
authors.initials: LF
authors.lastname: Farah
mesh_terms: D000091:Acetobacter / Q000378:metabolism; D001458:Bandages; D001684:Biological Dressings; D002482:Cellulose / Q000096:biosynthesis / Q000627:therapeutic use*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D012867:Skin; D014961:X-Ray Diffraction
chemical_list: D002482:Cellulose