The determination of IgE synthesis in vitro. Methodological aspects.
Various methods to determine net synthesis of IgE in cell cultures and to differentiate this from preformed IgE have been evaluated. The most accurate values for net synthesis were obtained by adding the IgE levels in supernatants to the level of cell-bound acid extractable IgE at the end of the culture period and subtracting the IgE levels present in cultures subject to acid treatment on day 0.
Gamkrelidze
A
A
Department of Pediatrics, Faculty of Health Sciences, University Hospital, Linköping, Sweden.
Björkstén
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B
D000293:Adolescent; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D007073:Immunoglobulin E / Q000032:analysis / Q000096:biosynthesis*
D007073:Immunoglobulin E; D003513:Cycloheximide