Influence of beta-adrenergic stimulation on glycosylation of a major, secretory N-linked glycoprotein from rat parotid salivary gland.
beta-Adrenergic stimulation with 10 microM isoproterenol increased the [3H]-mannose/[14C]-leucine ratio (three- to six-fold) of protein extracts in double-radiolabelled rat parotid acinar cells. Characteristics of oligosaccharides in a major parotid glycoprotein (Mr approximately 220,000; gp 220) were studied. Gp 220 from control and experimental cells was endoglycosidase H-insensitive, endoglycosidase F-sensitive and bound both concanavalin A and wheat germ agglutinin. Gp 220 was removed from concanavalin A-Sepharose by sequential elution with 10 mM alpha-methyl glucoside and 0.5 M alpha-methyl mannoside. These findings suggest that (1) oligosaccharides in gp 220 have both a biantennary complex and hybrid oligosaccharide chains, and (2) beta-adrenoreceptor stimulation has little effect on the gross oligosaccharide structures of this glycoprotein.
Baum
B J
BJ
Clinical Investigations and Patient Care Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892.
Yeh
C K
CK
Kousvelari
E E
EE
D000818:Animals; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000378:metabolism*; D006031:Glycosylation; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D009844:Oligosaccharides / Q000378:metabolism; D010306:Parotid Gland / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012471:Salivary Proteins and Peptides / Q000378:metabolism*
D006023:Glycoproteins; D009844:Oligosaccharides; D012471:Salivary Proteins and Peptides; D007545:Isoproterenol