Molecular cloning and sequence analysis of cDNA encoding the macrophage lectin specific for galactose and N-acetylgalactosamine.
The primary structure of the macrophage lectin specific for galactose and N-acetylgalactosamine (macrophage asialoglycoprotein-binding protein, M-ASGP-BP) has been deduced from its cDNA sequence. The M-ASGP-BP cDNA encoded a protein consisting of 306 amino acid residues with a molecular mass of 34,242 daltons. The sequence was highly homologous with that of the rat liver asialoglycoprotein receptor (rat hepatic lectin, RHL), particularly that of RHL-1 (the major form of RHL), throughout its whole length, and especially so in its putative membrane-spanning region and carbohydrate recognition domain. There were two N-glycosylation sites in M-ASGP-BP, the location of which were identical to those in RHL-1. However, M-ASGP-BP was characteristic in having a shorter cytoplasmic tail, and an inserted segment of 24 amino acids containing an Arg-Gly-Asp sequence between the membrane-spanning region and carbohydrate recognition domain.
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Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Kurata
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Itoh
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Yamashina
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Kawasaki
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D000116:Acetylgalactosamine / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D037263:Asialoglycoprotein Receptor; D001212:Asialoglycoproteins; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D003062:Codon; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D005688:Galactosamine / Q000031:analogs & derivatives*; D005690:Galactose / Q000378:metabolism*; D006031:Glycosylation; D037102:Lectins / Q000235:genetics*; D037181:Lectins, C-Type; D008264:Macrophages / Q000032:analysis; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D051381:Rats; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D012689:Sequence Homology, Nucleic Acid
D037263:Asialoglycoprotein Receptor; D001212:Asialoglycoproteins; D002352:Carrier Proteins; C064720:Clec10a protein, mouse; C023339:Clec10a protein, rat; D003062:Codon; D037102:Lectins; D037181:Lectins, C-Type; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D005688:Galactosamine; D004247:DNA; D000116:Acetylgalactosamine; D005690:Galactose