Separation of non-collagenous proteins of bone formed in titanium implants: experimental study in the rabbit tibia, using a bone harvest chamber.
The non-collagenous proteins of mature cortical rabbit bone and 3 wk old bone specimens formed in titanium implant bone harvest chambers, were separated by means of fine pressure liquid chromatography. It was found that a sufficient amount of bone could be obtained from five bone harvest chambers in five rabbits during a period of 15 wk. A different protein chromatographic pattern was found in bone grown in bone harvest chambers as compared to normal cortical bone from the same region. This indicates a difference in bone quality and shows that this implant design can be used for biochemical analysis of bone formed adjacent to a biomaterial.
Lundgren
T
T
Department of Oral Biochemistry, Gothenburg University, Sweden.
Sennerby
L
L
D000818:Animals; D001846:Bone Development / Q000502:physiology; D001842:Bone and Bones / Q000032:analysis*; D002850:Chromatography, Gel; D019736:Prostheses and Implants; D011506:Proteins / Q000302:isolation & purification*; D011817:Rabbits; D013977:Tibia / Q000032:analysis; D014025:Titanium
D011506:Proteins; D014025:Titanium