Estrogen regulation of placental alkaline phosphatase gene expression in a human endometrial adenocarcinoma cell line.
A human endometrial tumor (Ishikawa) cell line in culture responded to estradiol stimulation, as measured by growth and alkaline phosphatase activity. These effects were similar whether the medium was enriched with serum or was serum-free. Estradiol increased placental alkaline phosphatase activity 2-3-fold over control in these Ishikawa cells. The mechanism for this increase appeared to be at the level of transcription, at least in part, since there was an increase in the concentration of placental alkaline phosphatase mRNA. The administration of tamoxifen or 4-hydroxytamoxifen was unable to antagonize the estradiol-stimulated alkaline phosphatase enzyme activity or mRNA expression. The administration of tamoxifen alone had no effect on alkaline phosphatase enzyme activity, but tamoxifen did stimulate the steady state concentration of alkaline phosphatase mRNA. In contrast, a new antiestrogen, ICI 164,384, was able to antagonize both of these estradiol-stimulated effects.
Albert
J L
JL
Department of Obstetrics and Gynecology, University of Pennsylvania, Philadelphia 19104.
Sundstrom
S A
SA
Lyttle
C R
CR
D000230:Adenocarcinoma / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D000469:Alkaline Phosphatase / Q000235:genetics* / Q000378:metabolism; D002452:Cell Count; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology*; D004965:Estrogen Antagonists / Q000494:pharmacology; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D010920:Placenta / Q000201:enzymology*; D053284:Polyunsaturated Alkamides; D012333:RNA, Messenger / Q000032:analysis*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology; D014594:Uterine Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology
D004965:Estrogen Antagonists; D007527:Isoenzymes; D053284:Polyunsaturated Alkamides; D012333:RNA, Messenger; D004958:Estradiol; C051781:ICI 164384; D000469:Alkaline Phosphatase