DNA polymerase alpha does not mediate G0-G1 increase in yield of X-ray-induced exchange aberrations in human peripheral blood lymphocytes.
We report experiments to test the hypothesis that the increased yield of dicentric chromosomes observed in human peripheral blood lymphocytes treated with X-rays during the G1 phase of their first cell cycle, as compared with the yield when the cells are treated in their G0 phase prior to phytohemagglutinin stimulation, is a manifestation of the recently-reported conversion of an inactive form of DNA polymerase alpha to its active form as the PHA-stimulated cells pass from G0 into G1 (Sylvia et al., 1988). The specific polymerase alpha inhibitor butylphenyl deoxyguanosine was used as an X-ray post-treatment. The results show that polymerase alpha is not involved.
Bender
M A
MA
Medical Department, Brookhaven National Laboratory, Upton, NY 11973.
Moore
R C
RC
D002453:Cell Cycle / Q000528:radiation effects; D002869:Chromosome Aberrations; D002872:Chromosome Deletion; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D003848:Deoxyguanine Nucleotides / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects / Q000528:radiation effects*; D010835:Phytohemagglutinins / Q000494:pharmacology
D003848:Deoxyguanine Nucleotides; D010835:Phytohemagglutinins; C040364:N(2)-(4-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate; D004257:DNA Polymerase II; D004121:Dimethyl Sulfoxide