Transcription of biliary glycoprotein I gene in malignant and non-malignant human liver tissues.
Biliary glycoprotein I (BGP I) is a member of carcinoembryonic antigen (CEA) gene family consisting of at least 11 related genes. The transcription of BGP I gene was analysed in malignant and non-malignant human liver tissues with a 396-bp 3'-untranslated region probe from a cDNA clone 4-13 which was newly isolated from an adult human colon cDNA library. Among 21 tissue samples from 14 patients with hepatocellular carcinoma, 16 samples were clearly shown to express a single 3.9-kb message. This message was also found in the hepatoma cell line HuH-7. When the malignant tissues were compared to the non-malignant ones for the intensity of the band, no significant difference was observed. mRNAs of CEA and non-specific cross-reacting antigen (NCA) were not detected in 5 samples which were shown to have the message of the BGP I gene. These data suggest that the human hepatocyte and its malignant transformant produce BGP I, and that this could correspond to the cross-reacting antigen previously detected in the liver.
Hinoda
Y
Y
Department of Internal Medicine (Section 1), Sapporo Medical College, Japan.
Imai
K
K
Nakagawa
N
N
Ibayashi
Y
Y
Nakano
T
T
Paxton
R J
RJ
Shively
J E
JE
Yachi
A
A
D015703:Antigens, CD; D000951:Antigens, Neoplasm / Q000235:genetics; D002272:Carcinoembryonic Antigen / Q000235:genetics; D015815:Cell Adhesion Molecules; D002460:Cell Line; D015342:DNA Probes; D015723:Gene Library; D005796:Genes; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism*; D008970:Molecular Weight; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D015183:Restriction Mapping; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured / Q000378:metabolism
D015703:Antigens, CD; D000951:Antigens, Neoplasm; C069847:CD66 antigens; D002272:Carcinoembryonic Antigen; D015815:Cell Adhesion Molecules; D015342:DNA Probes; D006023:Glycoproteins; D012333:RNA, Messenger