title: Usefulness of fibroblast culture for testing of cattle tissues polluted with heavy metals.
abstract: Cattle tissues (liver, kidney, brain, and lung) that had been polluted with heavy metals were tested for their ability to alter fibroblast culture growth, cellular protein and DNA content, and fibroblast DNA synthesis. At 72 hr of incubation a significant increase in cellular DNA and [14C]thymidine incorporation was noted in the primary cultures as well as in the subcultures compared to controls. Fibroblast cultures also displayed growth inhibition and reduction in protein content. The measurement of basic biochemical parameters of the fibroblast culture may represent a sensitive means of assessing rapidly the activity of heavy metals deposited in the tissues of cattle as a result of their grazing on polluted soil.
authors.affiliation: Department of Biochemistry and Chemistry, Medical University School of Silesia, Katowice, Poland.
authors.forename: L
authors.initials: L
authors.lastname: Weglarz
authors.forename: M
authors.initials: M
authors.lastname: Drózdz
authors.forename: M
authors.initials: M
authors.lastname: Wardas
authors.forename: B
authors.initials: B
authors.lastname: Kula
authors.forename: M
authors.initials: M
authors.lastname: Pawlaczyk-Szpilowa
mesh_terms: D000818:Animals; D002250:Carbon Radioisotopes; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis / Q000096:biosynthesis; D005347:Fibroblasts / Q000032:analysis / Q000187:drug effects* / Q000378:metabolism; D008670:Metals / Q000032:analysis* / Q000493:pharmacokinetics / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D012989:Soil Pollutants / Q000032:analysis*; D013936:Thymidine / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D002250:Carbon Radioisotopes; D008670:Metals; D012989:Soil Pollutants; D004247:DNA; D013936:Thymidine