Synthesis of phosphonate derivatives of myo-inositol for use in biochemical studies of inositol-binding proteins.
Phospholipids containing the inositol headgroup (phosphoinositides) serve as membrane storage forms of a family of messenger molecules that transmit signals in cells. In this study a general synthesis of myo-inositol phosphate derivatives in which the phosphorus oxygen bond is replaced with a phosphorus carbon bond (i.e. phosphonates) is presented. Four specific examples of phosphonate analogs of phosphatidylinositol (PI) are prepared which have a single alkyl chain in place of the diacylglycerol. These derivatives are stable in neutral and alkaline solutions and are designed for use in biochemical studies of PI-specific phospholipases C and other enzymes involved in the phosphoinositide signal transduction pathway.
Shashidhar
M S
MS
Institute of Molecular Biology, University of Oregon, Eugene 97403.
Keana
J F
JF
Volwerk
J J
JJ
Griffith
O H
OH
D055598:Chemical Phenomena; D002621:Chemistry; D007294:Inositol / Q000031:analogs & derivatives*; D010716:Phosphatidylinositols
D010716:Phosphatidylinositols; D007294:Inositol