title: Healing of polytetrafluoroethylene arterial grafts is influenced by graft porosity.
abstract: The importance of porosity in synthetic arterial graft healing has not been adequately defined. To determine the effect of porosity on graft healing, we measured the extent of cellular response at late times in 4 mm internal diameter polytetrafluoroethylene grafts of varying porosity (between 10 and 90 microns internodal distance) inserted into the arterial system of baboons. After 1 and 3 months the grafts were retrieved and examined for endothelial coverage, intimal thickening, and endothelial cell and smooth muscle cell proliferation. The pattern of intimal healing with endothelial cells and smooth muscle cells was only related to porosity in the sense that there was an abrupt switch in the pattern of healing as porosity was increased from 30 to 60 microns. In low porosity grafts (10 and 30 microns internodal distances) endothelial coverage of the luminal surface was incomplete and, along with intimal thickening, was limited to graft near the anastomosis. In high porosity grafts (60 and 90 microns internodal distances) luminal endothelial coverage was complete, and intimal thickening was uniformly distributed throughout the graft. The highest porosity graft studied (90 microns) developed areas of focal loss of endothelial cells at late time periods. In this limited series there does appear to be an optimal porosity for polytetrafluoroethylene grafts near 60 microns, since 10 and 30 micron grafts fail to achieve luminal endothelial cell coverage, and 90 micron grafts exhibit instability of the intima with focal endothelial cell loss.
authors.affiliation: Department of Surgery, University of Washington, School of Medicine, Seattle 98195.
authors.forename: M A
authors.initials: MA
authors.lastname: Golden
authors.forename: S R
authors.initials: SR
authors.lastname: Hanson
authors.forename: T R
authors.initials: TR
authors.lastname: Kirkman
authors.forename: P A
authors.initials: PA
authors.lastname: Schneider
authors.forename: A W
authors.initials: AW
authors.lastname: Clowes
mesh_terms: D000818:Animals; D001345:Autoradiography; D001807:Blood Vessel Prosthesis; D004730:Endothelium, Vascular / Q000502:physiology; D008297:Male; D008855:Microscopy, Electron, Scanning; D009131:Muscle, Smooth, Vascular / Q000502:physiology; D010215:Papio; D011138:Polytetrafluoroethylene; D013499:Surface Properties; D014945:Wound Healing
chemical_list: D011138:Polytetrafluoroethylene