A spectrophotometric method for detection of 5'-fluorouracil in plasma.
A quantitative method using spectrophotometry was devised for the detection of 5'-Fluorouracil (FUra) in plasma. Plasma samples obtained at different time intervals from drug treated rabbits were deproteinised using phenol. The aqueous phase was lyophilised and dissolved in 0.01 N NaOH and absorption was monitored at 305 nm. Levels of FUra in the plasma were calculated by comparing with a standard graph generated from known concentrations of FUra. As low as 1 microgram of the drug per ml of plasma could be detected by this method.
Kumar
A M
AM
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore.
Nayak
R
R
D000818:Animals; D002855:Chromatography, Thin Layer; D005472:Fluorouracil / Q000097:blood*; D008297:Male; D011817:Rabbits; D013057:Spectrum Analysis / Q000379:methods*
D005472:Fluorouracil