Structure of chicken aldolase C mRNA and its expression in the liver and brain during development.
Chicken aldolase B (liver-type) and C (brain-type) cDNAs were isolated and their nucleotide sequences determined. Southern blot analysis of chicken genomic DNA using the fragments of aldolase C cDNA suggested that the aldolase C gene is a single-copy gene. The quantification by Northern blot analysis of aldolase B and C mRNAs in the chicken liver and brain during development showed that in the liver, the B gene was progressively activated, while C gene expression was extinguished reciprocally. In the brain, the B gene was silent throughout the development, while the C gene was activated progressively, reaching a product level of more than 20-fold that of the 7-day-old embryo.
Ono
K
K
Department of Biochemistry, Yamagata University School of Medicine, Japan.
Tsutsumi
K
K
Ishikawa
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology / Q000254:growth & development*; D002645:Chickens; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics*; D015870:Gene Expression; D007527:Isoenzymes / Q000235:genetics*; D008099:Liver / Q000201:enzymology / Q000254:growth & development*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D007527:Isoenzymes; D012333:RNA, Messenger; D004247:DNA; D005634:Fructose-Bisphosphate Aldolase