Morphological alterations induced by doxorubicin in B16 melanoma cells.
In B16 melanoma, cells morphologically different can be distinguished. In order to establish possible correlations between cell morphology and drug-response, the cytotoxic response to doxorubicin was analyzed. The two subpopulations, represented by two types of colonies, showed a different degree of sensitivity to doxorubicin. Moreover, following treatment, colonies strongly altered in their morphology were found, suggesting a differentiating activity of doxorubicin (dendritic prolongations, increase of intracellular melanin, block of cell proliferation). These results suggest that doxorubicin, besides having a different cytotoxic effect on the two cell subpopulations, induces in this cell line morphological alterations consistent with a differentiation process.
Mariani
M
M
Division of Experimental Oncology B, Istituto Nazionale Tumori, Milan, Italy.
Supino
R
R
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000494:pharmacology*; D008543:Melanins / Q000032:analysis; D008545:Melanoma / Q000473:pathology* / Q000648:ultrastructure; D051379:Mice; D013997:Time Factors; D014407:Tumor Cells, Cultured / Q000187:drug effects
D008543:Melanins; D004317:Doxorubicin