title: Effects of gallopamil on calcium release and intramembrane charge movements in frog skeletal muscle fibres.
abstract: 1. Intramembrane charge movements and changes in intracellular Ca2+ concentration were studied in voltage clamp experiments on cut twitch muscle fibres of the frog. The restoration from inactivation caused by steady depolarization and its modification by the phenylalkylamine Ca2+ channel antagonist gallopamil (D600, 10-30 microM) were investigated. 2. D600 prevented the restoration from inactivation of Ca2+ release which normally occurred at -80 mV. In D600 Ca2+ release recovered from inactivation at -120 mV. 3. D600 did not alter the characteristics of intramembrane charge movements in the depolarized fibre (charge 2) but the increase in the amount of mobile charge in the test voltage range above -60 mV, which normally occurs after changing the holding potential to -80 mV, was suppressed. The charge movement characteristics of D600-paralysed fibres, which were held at -80 mV, equalled those of normal depolarized and inactivated fibres. 4. Control records for the charge movement analysis were always obtained by voltage steps above 0 mV. Using the 'conventional' control in the potential range between -80 and -160 mV led to an underestimation and a kinetic deformation of charge movements in D600-treated fibres, which was due to various amounts of nonlinear charge in the control. 5. Like the restoration of Ca2+ release at -80 mV in normal fibres the recovery from paralysis at -120 mV in D600-treated fibres was accompanied by a significant increase in mobile charge in the potential range positive of -60 mV. Both Ca2+ release and charge movement at test potentials above -60 mV recovered with almost identical time course. 6. Restoration of Ca2+ release at a holding potential of -80 mV in normal fibres or at -120 mV in D600-treated fibres could not be clearly correlated to charge movement changes in the voltage range negative of -60 mV (charge 2). 7. Our results are consistent with a voltage-dependent inhibitory effect of D600 on the charge displacement that controls Ca2+ release from the sarcoplasmic reticulum but provide little evidence for a conversion of charge 2 into the charge that is involved in the control of Ca2+ release.
authors.affiliation: Department of Cell Physiology, Ruhr-University Bochum, FRG.
authors.forename: D
authors.initials: D
authors.lastname: Feldmeyer
authors.forename: W
authors.initials: W
authors.lastname: Melzer
authors.forename: B
authors.initials: B
authors.lastname: Pohl
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004396:Coloring Agents; D005711:Gallopamil / Q000494:pharmacology*; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects; D009132:Muscles / Q000502:physiology*; D009282:Naphthalenesulfonates; D011896:Rana temporaria; D013997:Time Factors
chemical_list: D004396:Coloring Agents; D009282:Naphthalenesulfonates; D005711:Gallopamil; C016238:antipyrylazo III; D002118:Calcium