Fluorescence polarization immunoassay for cyclosporine A determination in whole blood.
A fluorescence polarization immunoassay (FPIA) for cyclosporine A (CsA) determination in blood was evaluated. The method demonstrated a good reproducibility both in within-run and between-run assays. The linearity range was 35-990 micrograms/L. Comparison of measurements in patients' whole blood with radioimmunoassay results showed higher estimates by the FPIA despite the good correlation coefficient. Finally, a poor agreement was observed between the evaluated method and the high-performance liquid chromatographic technique for CsA determination.
Plebani
M
M
Department of Clinical Biochemistry, University of Padova, Italy.
Paleari
C D
CD
Masiero
M
M
Faggian
D
D
Burlina
A
A
D000293:Adolescent; D000328:Adult; D002648:Child; D002851:Chromatography, High Pressure Liquid; D003524:Cyclosporins / Q000097:blood*; D005069:Evaluation Studies as Topic; D005454:Fluorescence Polarization / Q000379:methods*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008875:Middle Aged; D011863:Radioimmunoassay; D015203:Reproducibility of Results
D003524:Cyclosporins