title: Ultracytochemistry of glycoconjugates in rat Brunner's gland with labeled lectins.
abstract: Mucous glycoproteins of rat Brunner's gland were examined ultracytochemically to elucidate the intracellular localization and sites of glycosylation in relation to the functional polarity of the cell organellae using a combination of hydrophilic resin embedment and postembedding staining with labeled lectins. A few cis cisternae were stained with HPA (Helix pomatia agglutinin, specific for terminal alpha-N-acetylgalactosamine) and DBA (Dolichos biflorus aggulutinin, specific for terminal alpha-N-acetylgalactosamine). Trans cisternae as well as cisternae following secretory granules were stained with HPA and RCA-I (Recinus communis agglutinin I, specific for terminal beta-galactose). UEA-I (Ulex europaeus agglutinin I, specific for terminal alpha-L-fucose) seemed to bind to transmost cisternae rather than trans cisternae. HPA bound to the whole cisternae, but to the cis or trans cisternae depending on a cell type. Apical mucous granules were stained with UEA-I, RCA-I, and HPA, but not with DBA. DGA seemed to be a good marker of the cis side of the Golgi apparatus and cell membrane. The glycosylation of mucous glycoprotein in rat Brunner's gland was partly clarified electron microscopically.
authors.affiliation: Department of Dermatology, Faculty of Medicine, Kagoshima University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Yotsumoto
authors.forename: S
authors.initials: S
authors.lastname: Tsuyama
authors.forename: M
authors.initials: M
authors.lastname: Tashiro
authors.forename: F
authors.initials: F
authors.lastname: Murata
mesh_terms: D000818:Animals; D002011:Brunner Glands / Q000378:metabolism / Q000648:ultrastructure*; D004386:Duodenum / Q000648:ultrastructure*; D005260:Female; D006001:Glycoconjugates / Q000378:metabolism*; D006651:Histocytochemistry / Q000379:methods; D037102:Lectins / Q000378:metabolism*; D008297:Male; D008854:Microscopy, Electron / Q000379:methods; D051381:Rats
chemical_list: D006001:Glycoconjugates; D037102:Lectins