Replication-linked histone acetylation in rat liver tissue is sensitive to alkylating agents.
The effect of alkylating agents on histone acetyltransferase (EC 2.3.1.48) activity and thymidine incorporation was investigated in benign and malignant proliferating rat liver tissue and compared with the effect in normal non-proliferating rat liver tissue. In both, benign and malignant proliferating tissue, but not in quiescent tissue, the histone acetylation is depressed by alkylating agents and this depression correlates with the inhibition of the thymidine incorporation. This effect suggests that the depression of the replication associated histone acetylation may be an important factor for the antiproliferative activity of alkylating agents.
Talasz
H
H
Institut für Medizinische Chemie und Biochemie, Universität Innsbruck, Austria.
Weiss
G
G
Puschendorf
B
B
D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism*; D000477:Alkylating Agents / Q000494:pharmacology*; D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D004261:DNA Replication / Q000187:drug effects*; D051548:Histone Acetyltransferases; D006657:Histones / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D029701:Saccharomyces cerevisiae Proteins; D013936:Thymidine / Q000378:metabolism
D000477:Alkylating Agents; D006657:Histones; D029701:Saccharomyces cerevisiae Proteins; D000123:Acetyltransferases; D051548:Histone Acetyltransferases; D013936:Thymidine