[Association of [3H]-dexamethasone with cytoplasmic receptor proteins and their translocation into liver cell nuclei during aging].
Interaction of 3H-dexamethasone with cytoplasmic proteins was studied in male rat liver cells. With ageing of the animals concentration of high affinity and low affinity glucocorticoid receptors was decreased 2-fold as well as the corresponding reduction of nuclear uptake in the steroid-receptor complexes was found. A protein species was found in cytoplasm of aged rat hepatocytes, which bound 3H-dexamethasone with "extremely low" affinity and was not able to associate with the cell nuclei.
Fil'chenkov
G N
GN
D000375:Aging / Q000378:metabolism*; D000818:Animals; D001667:Binding, Competitive; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D003907:Dexamethasone / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000378:metabolism*
D011965:Receptors, Glucocorticoid; C028697:dexamethasone receptor; D003907:Dexamethasone
Assotsiatsiia [3H]-deksametazona tsitoplazmaticheskimi retseptornymi belkami i ikh translokatsiia v iadra kletok pecheni pri starenii.