Cross-reactive and specific monoclonal antibodies against cellobiohydrolases I and II and endoglucanases I and II of Trichoderma reesei.
Splenocytes derived from mice inoculated with a commercial cellulase preparation or purified cellulases were fused with a stable myeloma cell line (SP2/0). Specific monoclonal antibodies to cellobiohydrolases I and II and endoglucanases I and II were established. In addition to specific monoclonal antibodies, we were also able to establish stable hybridoma cell lines which produced monoclonal antibodies that recognized similar epitopes possessed by two or more of the above cellulases. By obtaining monospecific antibodies for all four individual cellulases, the role and function of the individual cellulases can thus be studied in greater detail.
Nieves
R A
RA
Department of Microbiology, Colorado State University, Fort Collins 80525.
Himmel
M E
ME
Todd
R J
RJ
Ellis
R P
RP
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D015153:Blotting, Western; D002480:Cellulase / Q000276:immunology*; D043366:Cellulose 1,4-beta-Cellobiosidase; D003429:Cross Reactions; D006026:Glycoside Hydrolases / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D007527:Isoenzymes / Q000276:immunology; D051379:Mice; D003904:Mitosporic Fungi / Q000201:enzymology*; D014242:Trichoderma / Q000201:enzymology*
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D006026:Glycoside Hydrolases; D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase