Parameters affecting hybridization of nucleic acids blotted onto nylon or nitrocellulose membranes.
Nine different nylon and nitrocellulose membranes were compared utilizing four different methods of attaching the nucleic acid target. Nylon membranes repeatedly demonstrated increased sensitivity as compared to nitrocellulose membranes. Sensitivity could also be enhanced by mildly denaturing the target prior to attachment onto the membrane. This was achieved by either UV cross-linking or baking.
Twomey
T A
TA
Department of Molecular Biology and Genetics, Wayne State University, Detroit, MI 48201.
Krawetz
S A
SA
D003101:Collodion; D004247:DNA / Q000032:analysis; D015151:Immunoblotting / Q000379:methods*; D008567:Membranes, Artificial; D009693:Nucleic Acid Hybridization; D009757:Nylons
D008567:Membranes, Artificial; D009757:Nylons; D003101:Collodion; D004247:DNA