title: Sialoconjugates and development of the tail bud.
abstract: Using lectin histochemistry, we have previously shown that there are alterations in the distribution of glycoconjugates in the tail bud of chick embryos that parallel the developmental sequence of the caudal axis. If glycoconjugates or the cells bearing them play a role in caudal axial development, then, restriction of their availability by binding with lectins would be expected to produce abnormalities of caudal development. In the present study, we treated embryos at various stages of tail bud development by microinjection with a variety of lectins. Administration of WGA by sub-blastodermal injection resulted in high incidences of secondary neural tube and notochordal abnormalities in lectin-treated embryos. The incidence of malformations was dependent upon both the dose of WGA received and the stage of development at the time of treatment. Using an anti-WGA antibody, we have also shown binding of the lectin in regions where defects were found. The lectin WGA binds to the sialic acid residues of glycoconjugates and to N-acetylglucosamine. Treatment of embryos with Limulus polyphemus lectin (LPL), which also binds to sialic acid, produced results similar to those of WGA. Treatments using lectins with other sugar-binding specificities, including succinylated WGA (with N-acetylglucosamine specificity only) produced defects that differed from those produced by WGA and LPL, and only with the administration of much higher doses. The results suggest that glycoconjugates in general and sialoconjugates in particular, or the cells carrying them, may have a role in caudal axial development.
authors.affiliation: Department of Anatomy, University of Toronto, Ontario, Canada.
authors.forename: C M
authors.initials: CM
authors.lastname: Griffith
authors.forename: M J
authors.initials: MJ
authors.lastname: Wiley
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000187:drug effects / Q000196:embryology* / Q000648:ultrastructure; D002642:Chick Embryo; D005314:Embryonic and Fetal Development / Q000187:drug effects / Q000502:physiology*; D006001:Glycoconjugates / Q000502:physiology*; D008854:Microscopy, Electron; D019158:N-Acetylneuraminic Acid; D012794:Sialic Acids / Q000378:metabolism; D014909:Wheat Germ Agglutinins / Q000378:metabolism / Q000494:pharmacology
chemical_list: D006001:Glycoconjugates; D012794:Sialic Acids; D014909:Wheat Germ Agglutinins; D019158:N-Acetylneuraminic Acid