title: An improved method for the analysis of data from radiation-inactivation studies.
abstract: This paper addresses statistical issues in the estimation of protein molecular weight using radiation-inactivation assays. In particular it considers experiments in which a number of internal standards are used to supplement or replace accurate measurement of the applied doses of radiation. A mathematical model is proposed which allows the use of the standard technique of maximum-likelihood estimation to estimate the unknown molecular weight without knowledge of the applied doses and, in contrast to previous methods, allows the construction of confidence intervals.
authors.affiliation: Scottish Agricultural Statistics Service, Edinburgh.
authors.forename: I
authors.initials: I
authors.lastname: Bradbury
authors.forename: V A
authors.initials: VA
authors.lastname: Zammit
mesh_terms: D000818:Animals; D002334:Carnitine O-Palmitoyltransferase / Q000378:metabolism; D016001:Confidence Intervals; D008316:Malonyl Coenzyme A / Q000378:metabolism; D008433:Mathematics; D008565:Membrane Proteins / Q000528:radiation effects; D008930:Mitochondria, Liver / Q000201:enzymology; D008970:Molecular Weight; D011506:Proteins / Q000528:radiation effects*; D051381:Rats; D012015:Reference Standards
chemical_list: D008565:Membrane Proteins; D011506:Proteins; D008316:Malonyl Coenzyme A; D002334:Carnitine O-Palmitoyltransferase