[Study of the optimal conditions of preservation of cephalosporin C-producing fungus Acremonium chrysogenum].
The influence of various preservation conditions of viability and antibiotic activity of Acremonium chrysogenum 281A and 305A strains producing cephalosporin C was studied. Cryogenization of the culture in the form of suspension of the vegetative mycelium in 20 per cent glycerol solution at a rate of 1 degree/min showed to be advantageous over lyophilization and L-drying. Cryogenization under such conditions provided rather high viability of the culture and preservation of its initial antibiotic activity for the period of its storage for at least 1.5 years in liquid nitrogen at a temperature of -196 degrees C.
Sidiakina
T M
TM
Ustiuzhanina
S V
SV
Novikova
N D
ND
Gorin
S E
SE
Esipova
V V
VV
Kalakutskiĭ
L V
LV
D000164:Acremonium / Q000378:metabolism / Q000502:physiology*; D002511:Cephalosporins / Q000096:biosynthesis*; D015925:Cryopreservation; D005612:Freeze Drying; D066298:In Vitro Techniques; D007218:Industrial Microbiology / Q000379:methods*; D011309:Preservation, Biological / Q000379:methods*; D014022:Tissue Survival / Q000502:physiology
D002511:Cephalosporins; C025163:cephalosporin C
Issledovanie optimal'nykh rezhimov konservatsii kul'tury griba Acremonium chrysogenum--produtsenta tsefalosporina C.