Introduction of the beta isozyme of protein kinase C accelerates induced differentiation of murine erythroleukemia cells.
Induction of differentiation in murine erythroleukemia cells (MELCs) involves a protein kinase C (PKC)-mediated step. Vincristine-resistant cells respond more rapidly to hybrid polar/apolar inducers than the parental cells. These vincristine-resistant MELCs contain elevated levels of the beta isozyme of PKC (PKC-beta). Exogenous homologous murine PKC-beta, incorporated into permeabilized MELCs, accelerates induced differentiation. Neither rat PKC-beta, nor mouse PKC-alpha, nor rat PKC-alpha, incorporated into permeabilized MELCs, is effective in altering the kinetics of induced differentiation. This provides direct evidence for a rate-limiting role for this PKC isozyme during N,N'-hexamethylenebisacetamide-mediated induced differentiation of a transformed cell.
Melloni
E
E
Institute of Biological Chemistry, University of Genoa, Italy.
Pontremoli
S
S
Sparatore
B
B
Patrone
M
M
Grossi
F
F
Marks
P A
PA
Rifkind
R A
RA
D000081:Acetamides / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000201:enzymology; D002454:Cell Differentiation; D002460:Cell Line; D004351:Drug Resistance; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute; D007942:Leukemia, Experimental; D051379:Mice; D011493:Protein Kinase C / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D014407:Tumor Cells, Cultured / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D014750:Vincristine / Q000494:pharmacology
D000081:Acetamides; D007527:Isoenzymes; D014750:Vincristine; D011493:Protein Kinase C; C014026:hexamethylene bisacetamide