Microplate assay of glutathione s-transferase activity for resistance detection in single-mosquito triturates.
1. Optimum conditions are described for a simple, rapid microplate assay that measures glutathione s-transferase (GST) activity accurately and precisely in small portions of single mosquito homogenates. 2. Up to 10 assay replicates were possible for individual adults and larvae. Concentration of GST activity in the head/thorax region allows blood-fed mosquitoes with abdomens removed to be used in assays. 3. The method allows the use of GST activity as a biochemical character in comparative studies of populations. 4. The microplate assay detects elevated GST activities associated with DDT resistance in Anopheles arabiensis.
Brogdon
W G
WG
Division of Parasitic Diseases, Centers for Disease Control, Atlanta, GA 30333.
Barber
A M
AM
D000818:Animals; D000852:Anopheles / Q000201:enzymology*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004351:Drug Resistance; D005260:Female; D005982:Glutathione Transferase / Q000378:metabolism*; D007814:Larva; D013045:Species Specificity
D005982:Glutathione Transferase