Kinetic studies of pyruvate kinase during in vitro differentiation of GM-CFC haemopoietic precursor and bone marrow cells in mice.
Pyruvate kinase studies in the granulocyte-macrophage lineage during in vitro differentiation have been performed using culture techniques on GM-CFC cells and a study has also been done in bone marrow cells. The enzyme exhibits biphasic behaviour with respect to both of its substrates in cells derived from in vitro cultures at 5 and 7 days of incubation period. However in bone marrow cells these kinetics are only observed for ADP. The different kinetic behaviour of pyruvate kinase toward Fru-1,6-P2, Ala, Phe and ATP in the three cellular populations allows us to conclude that the expression of pyruvate kinase is associated with the differentiation of these cells.
Cuenllas
E
E
Departamento de Bioquímica, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
Gaitan
S
S
Bueren
J A
JA
Tejero
C
C
D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000409:Alanine / Q000378:metabolism; D000818:Animals; D001853:Bone Marrow / Q000201:enzymology*; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D005635:Fructosediphosphates / Q000378:metabolism; D006098:Granulocytes / Q000166:cytology* / Q000201:enzymology; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008264:Macrophages / Q000166:cytology* / Q000201:enzymology; D051379:Mice; D008807:Mice, Inbred BALB C; D010649:Phenylalanine / Q000378:metabolism; D010728:Phosphoenolpyruvate / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism*
D005635:Fructosediphosphates; D010649:Phenylalanine; D000244:Adenosine Diphosphate; D010728:Phosphoenolpyruvate; D000255:Adenosine Triphosphate; D011770:Pyruvate Kinase; D000409:Alanine