The effects of tetracaine on charge movement in fast twitch rat skeletal muscle fibres.
1. The effects of tetracaine, a local anaesthetic that inhibits muscle contraction, on membrane potential and intramembrane charge movements were investigated in fast twitch rat muscle fibres (extensor digitorum longus). 2. The resting membrane potentials of surface fibres from muscles bathed in isotonic Ringer solution containing 2 mM-tetracaine were well maintained, but higher concentrations of tetracaine caused a time-dependent fall of potential. Muscle fibres bathed in hypertonic solutions containing 2 mM-tetracaine were rapidly depolarized. In both isotonic and hypertonic solutions, the depolarizing effect of tetracaine could not be reversed. 3. Charge movement measurements were made using the middle-of-the-fibre voltage clamp technique. The voltage dependence of charge movements measured in cold isotonic solutions was well fitted by a Boltzmann distribution (Q(V) = Qmax/(1 + exp(-(V-V)/k] where Qmax = 37.3 +/- 2.8 nC muF-1, V = -17.9 +/- 1.2 mV and k = 12.6 +/- 0.8 mV (n = 6, 2 degrees C; means +/- S.E. of means). Similar values were obtained when 2 mM-tetracaine was added to the isotonic bathing fluid (Qmax = 40.6 +/- 2.3 nC microF-1, V = -14.1 +/- 1.3 mV, k = 15.3 +/- 0.8 mV; n = 8, 2 degrees C). 4. Charge movements measured around mechanical threshold in muscle fibres bathed in hypertonic solutions were reduced when 2 mM-tetracaine was added to the bathing fluid. The tetracaine-sensitive component of charge was well fitted with an unconstrained Boltzmann distribution which gave: Qmax = 7.5 nC microF-1, V = -46.5 mV, k = 5.5 mV. The e-fold rise of the foot of the curve was 9.3 mV.
Hollingworth
S
S
Department of Physiological Sciences, Newcastle University Medical School, Newcastle upon Tyne.
Marshall
M W
MW
Robson
E
E
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D005260:Female; D006982:Hypertonic Solutions; D007552:Isotonic Solutions; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D009119:Muscle Contraction; D009132:Muscles / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D013748:Tetracaine / Q000494:pharmacology*; D013997:Time Factors
D006982:Hypertonic Solutions; D007552:Isotonic Solutions; D013748:Tetracaine