Identification of beta-exotoxin production, plasmids encoding beta-exotoxin, and a new exotoxin in Bacillus thuringiensis by using high-performance liquid chromatography.
An improved high-performance liquid chromatography separation was developed to detect and quantify beta-exotoxin production in Bacillus thuringiensis culture supernatants. Exotoxin production was assigned to a plasmid in five strains, from three subspecies (B. thuringiensis subsp. thuringiensis serotype 1, B. thuringiensis subsp. tolworthi serotype 9, and B. thuringiensis subsp. darmstadiensis serotype 10). A new exotoxin, called type II beta-exotoxin in this report, was discovered in B. thuringiensis subsp. morrisoni serotype 8ab, purified, and partially characterized. This material is more specific than type I beta-exotoxin and is very active against the Colorado potato beetle, Leptinotarsa decemlineata.
Levinson
B L
BL
Ecogen Inc., Langhorne, Pennsylvania 19047.
Kasyan
K J
KJ
Chiu
S S
SS
Currier
T C
TC
González
J M
JM
D001413:Bacillus thuringiensis / Q000032:analysis*; D001427:Bacterial Toxins / Q000032:analysis*; D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid; D005098:Exotoxins / Q000032:analysis* / Q000096:biosynthesis / Q000302:isolation & purification; D010957:Plasmids
D001427:Bacterial Toxins; D005098:Exotoxins