Efficient method for the isolation by HPLC, of a major metabolite of the carcinogen 2-amino-3-methylimidazo [4,5-f]quinoline (IQ), the 5-hydroxy derivative.
Previous difficulties in the standard HPLC separation of the 5-hydroxy derivative, a major metabolite of the food mutagen and carcinogen 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), with the properties of a chelating agent, were resolved by utilizing a nonmetallic tubing and column and a BRP-1 stationary phase. This system separates the already known metabolites by reverse phase mode, but presents the unique advantage of resolving chelating compounds like 5-hydroxy-IQ as a single, clean peak. The procedure may be generally applicable to this class of chemicals.
Vavrek
M T
MT
American Health Foundation, Valhalla, NY 10595-1599.
Weisburger
J H
JH
D000818:Animals; D002273:Carcinogens / Q000493:pharmacokinetics*; D002614:Chelating Agents; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005243:Feces / Q000032:analysis; D005966:Glucuronidase; D006912:Hydroxyquinolines / Q000302:isolation & purification* / Q000652:urine; D008297:Male; D011804:Quinolines / Q000493:pharmacokinetics*; D051381:Rats; D011916:Rats, Inbred F344
D002273:Carcinogens; D002614:Chelating Agents; D006912:Hydroxyquinolines; D011804:Quinolines; C060372:5-hydroxy-2-amino-3-methylimidazo(4,5-f)quinoline; C029216:2-amino-3-methylimidazo(4,5-f)quinoline; D005966:Glucuronidase