Terminal deoxynucleotidyltransferase containing megadalton complex from young rat thymus nuclei: identification and characterization.
Nuclear matrix prepared from 2-3 week old rat thymuses contains tightly bound TdT activity which has been quantitatively solubilized with nonionic detergent and sonication. TdT is contained in a discrete complex with a sedimentation value of 23 S. The complex is retained on an anti-TdT antibody column and contains DNA ligase and 3'-5' exonuclease activities as well as DNA and several other proteins but is devoid of replicative DNA polymerases. Such a type of multienzyme complex is absent from the nuclear extracts of thymus prepared from older rats and also from liver and spleen extracts of young and old rats.
Pandey
V N
VN
Biochemistry Division, Bhabha Atomic Research Centre, Bombay, India.
Dave
V P
VP
Patil
M S
MS
Pradhan
D S
DS
Amrute
S B
SB
Modak
M J
MJ
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002467:Cell Nucleus / Q000201:enzymology; D002499:Centrifugation, Density Gradient; D004254:DNA Nucleotidyltransferases / Q000276:immunology / Q000378:metabolism*; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains; D012995:Solubility; D013379:Substrate Specificity; D013950:Thymus Gland / Q000201:enzymology*
D000906:Antibodies; D004254:DNA Nucleotidyltransferases