title: Effect of transforming growth factor beta on proliferation of L6 and embryonic porcine myogenic cells.
abstract: We have examined the effect of Transforming Growth Factor (TGF) beta on proliferation of L6 and embryonic porcine myogenic cells. Proliferation of L6 cells was suppressed by both TGF beta-1 and TGF beta-2 in a dose-dependent manner. Half-maximal suppression of proliferation occurred at .036 ng TGF beta-1/ml and .06 ng TGF beta-2/ml. Maximal inhibition (60% suppression of proliferation for TGF beta-1 and 52% for TGF beta-2) occurred between .1 and .3 ng/ml for each growth factor. Suppression of proliferation was completely abolished in the presence of an anti-TGF beta antibody that inhibited the biological activity of TGF beta-1 and TGF beta-2. When we evaluated the effect of TGF beta-1 on proliferation of embryonic porcine myogenic cells we obtained results which were very similar to those obtained for L6 cells. Insulin-like growth factor (IGF)-I stimulated proliferation of L6 cells in a dose-dependent manner in serum-free, defined medium. However as little as .02 ng TGF beta-1/ml detectably suppressed this stimulation and .3 ng TGF beta-1/ml caused a 60% reduction in cell number in cultures treated with 30 ng IGF-l/ml. Thus TGF beta-1 significantly suppressed IGF-I-stimulated proliferation of L6 cells.
authors.affiliation: Department of Animal Science, University of Minnesota, St. Paul 55108.
authors.forename: M S
authors.initials: MS
authors.lastname: Pampusch
authors.forename: J R
authors.initials: JR
authors.lastname: Hembree
authors.forename: M R
authors.initials: MR
authors.lastname: Hathaway
authors.forename: W R
authors.initials: WR
authors.lastname: Dayton
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D009132:Muscles / Q000166:cytology* / Q000187:drug effects / Q000196:embryology; D013552:Swine / Q000196:embryology*; D015291:Transforming Growth Factors / Q000494:pharmacology*
chemical_list: D007334:Insulin-Like Growth Factor I; D015291:Transforming Growth Factors