title: Factor B subtyping in sera and bloodstains by isoelectric focusing and immunoblotting.
abstract: The polymorphism of BF was investigated in 765 unrelated Japanese individuals by isoelectric focusing and immunoblotting. Besides five common subtypes three rare variants were observed. The allele frequencies were: BF*S = 0.8078, BF*FA = 0.1797, BF*FB = 0.0105, BF*Var. = 0.0020. The above method was successfully applied to subtyping BF in stored bloodstains. The determination limits were: at 4 degrees C 8 weeks, at room temperature 2 weeks and at 37 degrees C only 2 days after storage. The BF subtyping is of practical use in medicolegal individualization of unknown bloodstains.
authors.affiliation: Department of Legal Medicine, Yamanashi Medical University, Tamaho, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Kido
authors.forename: N
authors.initials: N
authors.lastname: Komatsu
authors.forename: Y
authors.initials: Y
authors.lastname: Kimura
authors.forename: M
authors.initials: M
authors.lastname: Oya
mesh_terms: D001788:Blood Grouping and Crossmatching / Q000379:methods*; D011415:Complement Factor B / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004792:Enzyme Precursors / Q000235:genetics*; D005787:Gene Frequency / Q000235:genetics; D005828:Genetics, Population; D006801:Humans; D015151:Immunoblotting; D007525:Isoelectric Focusing; D007564:Japan; D010641:Phenotype; D011110:Polymorphism, Genetic / Q000235:genetics*
chemical_list: D004792:Enzyme Precursors; D011415:Complement Factor B