title: Modulation by polyamines of DNA-dependent DNA polymerase activity from human serum.
abstract: 1. Spermine, spermidine and putrescine activated DNA-dependent DNA polymerase from human sera by 47-125% at the concentrations of 0.2, 3 and 30 mM, respectively. 2. The polyamines shifted the optimal MgCl2 concentration for the polymerase activity from 10 mM to more physiological 5 mM. 3. Histamine having amino and imino groups at both ends of the molecule also increased the DNA polymerase activity, while cyclopentylamine and n-butylamine showed no effects on the enzyme activity. 4. The stimulatory effect of polyamines on the DNA polymerase activity was more evident with poly(dC)p(dG) used as a template/primer than with poly(dA)p(dT).
authors.affiliation: Department of Biochemistry, Mie University School of Medicine, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Shimamura
authors.forename: H
authors.initials: H
authors.lastname: Hibasami
authors.forename: U
authors.initials: U
authors.lastname: Kano
authors.forename: S
authors.initials: S
authors.lastname: Watanabe
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Nakashima
mesh_terms: D004247:DNA / Q000096:biosynthesis; D004256:DNA Polymerase I / Q000097:blood*; D004789:Enzyme Activation / Q000187:drug effects; D006632:Histamine / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D015636:Magnesium Chloride / Q000494:pharmacology; D011067:Poly dA-dT / Q000378:metabolism; D011073:Polyamines / Q000494:pharmacology*; D011089:Polydeoxyribonucleotides / Q000378:metabolism; D011700:Putrescine / Q000494:pharmacology; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology; D013698:Templates, Genetic
chemical_list: D011073:Polyamines; D011089:Polydeoxyribonucleotides; D015636:Magnesium Chloride; D011067:Poly dA-dT; C019530:poly(dC-dG); D013096:Spermine; D006632:Histamine; D004247:DNA; D004256:DNA Polymerase I; D013095:Spermidine; D011700:Putrescine