Endothelial repair of posterior corneal wounds in rabbits. Influence of foetal calf serum in the culture medium.
The endothelial covering of experimental wounds on the posterior corneal surface has been studied in organ culture experiments. Defects involving the Descemet's membrane were produced, in addition to pure endothelial defects. The influence of foetal calf serum (FCS) on the healing process was examined. Wounds including the Descemet's membrane, about 1-2 X 3 mm, were not covered by cells after 12 days in FCS free medium. Such wounds were covered by endothelium after 6 days when cultivated in medium containing 10% FCS, and partly covered in 1% FCS. Pure endothelial defects, 4 mm in diameter, were cell-covered within 3-4 days irrespective of the presence of FCS.


CONCLUSIONS
the presence of FCS is required for the healing of defects involving the Descemet's membrane, and not required for the healing of pure endothelial defects.
Medin
H
H
University Eye Department, Rikshospitalet, University of Oslo, Norway.
Davanger
M
M
D000818:Animals; D002417:Cattle; D003470:Culture Media / Q000494:pharmacology; D003886:Descemet Membrane / Q000187:drug effects / Q000293:injuries*; D004728:Endothelium, Corneal / Q000187:drug effects / Q000293:injuries*; D005312:Fetal Blood; D009924:Organ Culture Techniques; D011817:Rabbits; D014945:Wound Healing / Q000187:drug effects
D003470:Culture Media