FMRF-NH2-like peptide is deficient in the pituitary gland of the Brattleboro rat.
Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe-NH2 (F-8-F-NH2), isolated from bovine brain, is an FMRF-NH2-like peptide with morphine-modulating activity. In the rat, F-8-F-NH2 immunoreactivity (IR) is highly localized in the neurohypophysis. In this study, F-8-F-NH2-IR was studied in the hypothalamo-neurohypophyseal system of an Arg8-vasopressin (AVP)-deficient animal, the Brattleboro (DI) rat, and the normal control Long-Evans (LE) strain. F-8-F-NH2-IR in the DI pituitary is below the level of detection in contrast to that in the LE (0.50 +/- 0.04 pmol/gland). Neuropeptide Y (NPY) levels are increased two-fold in the DI pituitary while AVP levels are below detection. The content of F-8-F-NH2-IR in the hypothalami and spinal cords of DI and LE rats is not statistically different, suggesting that the absence of F-8-F-NH2-IR in the Brattleboro pituitary is not due to a genetic defect in F-8-F-NH2 biosynthesis. The results of this study raise the question whether AVP could be involved in the regulation of F-8-F-NH2 immunoreactivity in the neurohypophysis.
Majane
E A
EA
Laboratory of Biochemical Genetics, NIMH Neuroscience Center, St. Elizabeths, Washington, DC 20032.
Yang
H Y
HY
D000704:Analysis of Variance; D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D007031:Hypothalamus / Q000378:metabolism; D008297:Male; D009478:Neuropeptide Y / Q000378:metabolism; D009842:Oligopeptides / Q000378:metabolism* / Q000502:physiology; D010902:Pituitary Gland / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats; D011910:Rats, Brattleboro; D013116:Spinal Cord / Q000378:metabolism
D009478:Neuropeptide Y; D009842:Oligopeptides; D001127:Arginine Vasopressin; C053651:phenylalanyl-leucyl-phenylalanyl-glutaminyl-prolyl-glutaminyl-arginyl-phenylalaninamide