tert-Butyl hydroperoxide-induced injury in isolated rat hepatocytes: a model for studying anti-hepatotoxic crude drugs.
Tert-butyl hydroperoxide induces in freshly isolated rat hepatocytes malonaldehyde formation and lacticodehydrogenase and aspartate amino-transferase leakage. This model, when adapted to crude extracts, permits the demonstration of both anti-lipoperoxidant and antihepatotoxic activity of reference products like quercetin and silymarin and plant extracts like Rosmarinus officinalis and Eschscholzia californica.
Joyeux
M
M
Centre des Sciences de l'Environnement, Université de Metz, France.
Rolland
A
A
Fleurentin
J
J
Mortier
F
F
Dorfman
P
P
D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D019684:Magnoliopsida / Q000032:analysis; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010545:Peroxides / Q000633:toxicity*; D010936:Plant Extracts / Q000494:pharmacology; D011398:Promethazine / Q000494:pharmacology; D011794:Quercetin / Q000494:pharmacology; D051381:Rats; D012838:Silymarin / Q000494:pharmacology; D020122:tert-Butylhydroperoxide
D010545:Peroxides; D010936:Plant Extracts; D012838:Silymarin; D008315:Malondialdehyde; D020122:tert-Butylhydroperoxide; D011794:Quercetin; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D011398:Promethazine