title: Distribution of B-6 vitamers in human milk during a 24-h period after oral supplementation with different amounts of pyridoxine.
abstract: The relative distribution of B-6 vitamers, separated by reverse-phase liquid chromatography, was examined in human milk during a 24-h period after supplementation with 2.5 or 15 mg pyridoxine hydrochloride. Consistently, pyridoxal (PL) was the predominate vitamer and the most responsive to vitamin B-6 intake. During 3-8 h after supplement ingestion, PL, pyridoxal phosphate, and pyridoxamine concentrations were significantly higher than at other times examined. In the first two periods after supplementation, PL as a percentage of total vitamin B-6 was slightly but significantly higher in milk from the group supplemented with 15 mg than from the group supplemented with 2.5 mg. With the exception of PL, the distribution of B-6 vitamers, expressed as percent of total vitamin B-6, was similar for the two supplemented groups at all times examined. Percentage PL of total vitamin B-6 in milk was approximately 25% lower in unsupplemented than in supplemented women.
authors.affiliation: Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907.
authors.forename: B R
authors.initials: BR
authors.lastname: Hamaker
authors.forename: A
authors.initials: A
authors.lastname: Kirksey
authors.forename: M W
authors.initials: MW
authors.lastname: Borschel
mesh_terms: D000284:Administration, Oral; D000328:Adult; D005260:Female; D006801:Humans; D007774:Lactation / Q000378:metabolism; D008895:Milk, Human / Q000032:analysis*; D011730:Pyridoxal / Q000378:metabolism; D011732:Pyridoxal Phosphate / Q000378:metabolism; D011733:Pyridoxamine / Q000031:analogs & derivatives / Q000378:metabolism; D011736:Pyridoxine / Q000008:administration & dosage / Q000378:metabolism*; D013997:Time Factors
chemical_list: D011730:Pyridoxal; D011732:Pyridoxal Phosphate; D011733:Pyridoxamine; D011736:Pyridoxine; C010627:pyridoxamine phosphate