title: Wheat germ agglutinin chromatography of GlcNac beta 1-3(GlcNAc beta 1-6)Gal and GlcNAc beta 1-3(GlcNAc beta 1-6)Gal beta 1-4GlcNAc, obtained by in vitro synthesis and by partial cleavage of teratocarcinoma poly-N-acetyllactosaminoglycans.
abstract: GlcNAc beta 1-3(GlcNAc beta 1-6) [14C(U)]Gal and GlcNAc beta 1-3(GlcNAc beta 1-6)[14C(U)]Gal beta 1-4GlcNAc were prepared by in vitro synthesis. They were characterized by enzymatic sequencing, by partial acid hydrolysis, and by periodate oxidation experiments. The two saccharides were isolated also from partial acid hydrolysates of metabolically labeled poly-N-acetyllactosaminoglycans of murine embryonal carcinoma cells (line PC 13). The tetrasaccharide was retarded in a column of agarose-linked wheat germ agglutinin; the trisaccharide was strongly bound. Chromatography in this column separated the trisaccharide into two distinct peaks, which represented interconvertible molecules. Together with our previous data on linear teratocarcinoma saccharides, these findings show that affinity chromatography with immobilized wheat germ agglutinin can be advantageously used in fractionating radiolabeled oligo-N-acetyllactosaminoglycans and saccharides related to them.
authors.affiliation: Department of Biochemistry, University of Helsinki, Finland.
authors.forename: A
authors.initials: A
authors.lastname: Seppo
authors.forename: L
authors.initials: L
authors.lastname: Penttilä
authors.forename: A
authors.initials: A
authors.lastname: Makkonen
authors.forename: A
authors.initials: A
authors.lastname: Leppänen
authors.forename: R
authors.initials: R
authors.lastname: Niemelä
authors.forename: J
authors.initials: J
authors.lastname: Jäntti
authors.forename: J
authors.initials: J
authors.lastname: Helin
authors.forename: O
authors.initials: O
authors.lastname: Renkonen
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity; D002854:Chromatography, Paper; D054278:Embryonal Carcinoma Stem Cells; D006025:Glycosaminoglycans; D008969:Molecular Sequence Data; D014411:Neoplastic Stem Cells; D009844:Oligosaccharides / Q000032:analysis* / Q000138:chemical synthesis; D011134:Polysaccharides; D013552:Swine; D013724:Teratoma; D014312:Trisaccharides / Q000032:analysis* / Q000138:chemical synthesis; D014407:Tumor Cells, Cultured; D014909:Wheat Germ Agglutinins
chemical_list: D006025:Glycosaminoglycans; D009844:Oligosaccharides; D011134:Polysaccharides; D014312:Trisaccharides; D014909:Wheat Germ Agglutinins; C064589:N-acetylglucosaminyl(beta1-3)-N-acetylglucosaminyl(1-6)-galactopyranosyl(1-4)-N-acetylglucosamine; C064588:N-acetylglucosaminyl(1-3)-N-acetylglucosaminyl(1-6)-galactopyranose; C037199:poly-N-acetyllactosamine