title: Increased in vitro responses of tracheal smooth muscle from hyperresponsive guinea pigs.
abstract: Repeated aerosol antigen challenge of previously sensitized guinea pigs induces airway hyperresponsiveness to inhaled acetylcholine. To determine the mechanism producing these airway changes and assuming that changes in the trachealis muscle reflect changes in muscle of the entire tracheobronchial tree, we examined the in vitro smooth muscle mechanics and morphometric parameters of tracheae from guinea pigs demonstrating hyperresponsiveness in vivo vs. tracheae from control guinea pigs. No differences between these groups were found in luminal volume at zero transmural pressure, passive pressure-volume characteristics, or area of airway wall. Smooth muscle areas were slightly less in tracheae from hyperresponsive guinea pigs. Tracheae from hyperresponsive guinea pigs had both significantly increased isovolumetric force generation and isobaric shortening compared with tracheae from controls when evaluated over the range of transmural pressures from -40 to 40 cmH2O. We conclude that the in vivo airway hyperresponsiveness induced with repeated antigen challenge is associated with both increased force generation and shortening of tracheal smooth muscle without increased muscle mass, suggesting enhanced contractile activity.
authors.affiliation: University of British Columbia Pulmonary Research Laboratory, St. Paul's Hospital, Vancouver, Canada.
authors.forename: K
authors.initials: K
authors.lastname: Ishida
authors.forename: P D
authors.initials: PD
authors.lastname: Paré
authors.forename: R J
authors.initials: RJ
authors.lastname: Thomson
authors.forename: R R
authors.initials: RR
authors.lastname: Schellenberg
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology; D005260:Female; D006168:Guinea Pigs; D009119:Muscle Contraction; D009130:Muscle, Smooth / Q000473:pathology / Q000503:physiopathology*; D010047:Ovalbumin / Q000276:immunology; D012130:Respiratory Hypersensitivity / Q000276:immunology / Q000473:pathology / Q000503:physiopathology*; D015656:Respiratory Mechanics / Q000502:physiology; D014132:Trachea / Q000473:pathology / Q000503:physiopathology*
chemical_list: D000941:Antigens; D010047:Ovalbumin