Determination of size and molecular weight distributions of lipoproteins using automatic image analysis and density gradient ultracentrifugation.
A Leitz-Tas automatic image analysis system has been used to investigate the size distributions of human lipoprotein particles isolated by density gradient ultracentrifugation. Computerized image analysis enabled us to obtain more precise determinations of modal diameter and overall range in diameter for each lipoprotein subspecies than those determined by classical methods. In addition, lipoprotein molecular weight distributions were calculated from measurements of flotation density and particle size distributions. This method offers certain advantages over other procedures used to characterize mean molecular weight with regard to the ability to define lipoprotein polydispersity. The method and its limitations are discussed and illustrated with results obtained with human lipoproteins.
Le Goff
D
D
Laboratoire de Biochimie, Université de Caen, URA CNRS 609, Caen, France.
Nouvelot
A
A
Chermant
J L
JL
D000328:Adult; D000818:Animals; D002417:Cattle; D002499:Centrifugation, Density Gradient; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008074:Lipoproteins / Q000302:isolation & purification; D008075:Lipoproteins, HDL / Q000302:isolation & purification; D008077:Lipoproteins, LDL / Q000302:isolation & purification; D008297:Male; D008970:Molecular Weight
D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL