Selective killing of malignant cells from leukemic patients by alkyl-lysophospholipid.
We studied the effects of the alkyl-lysophospholipid, 1-octadecyl-2-methyl-sn-glycerol-3-phosphocholine (ALP), on human leukemia cells from 56 patients with various leukemias and on normal bone marrow progenitors in order to assess the application of ALP as an in vitro marrow-purging agent. The tumoricidal activity was analyzed by the elimination of clonogenic leukemia cells (leukemic colony-forming cells), by the inhibition of the proliferative capacity [( 3H]thymidine incorporation) of leukemia cells, and by the elimination of viable leukemia cells measured with flow cytometry. The tumoricidal activity of ALP was dose and incubation time related, as, although to a lesser extent, held true for normal marrow progenitors. For some leukemias the ALP dose necessary for the elimination of 100% of the leukemic colony-forming cells is probably too toxic for normal marrow cells. The results of this study strongly support the possibility that ALP is a promising purging agent in the majority of patients with leukemias.
Verdonck
L F
LF
Department of Haematology, University Hospital Utrecht, The Netherlands.
Witteveen
E O
EO
van Heugten
H G
HG
Rozemuller
E
E
Rijksen
G
G
D000293:Adolescent; D000328:Adult; D000368:Aged; D001752:Blast Crisis; D001853:Bone Marrow / Q000187:drug effects; D016026:Bone Marrow Transplantation; D002648:Child; D003114:Colony-Forming Units Assay; D005260:Female; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000188:drug therapy* / Q000473:pathology; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010742:Phospholipid Ethers / Q000494:pharmacology*; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000188:drug therapy* / Q000473:pathology; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000188:drug therapy* / Q000473:pathology; D014182:Transplantation, Autologous; D014410:Tumor Stem Cell Assay
D010742:Phospholipid Ethers; C026659:edelfosine