Intraperitoneal administration of carrier erythrocytes in dogs: an improved method for delivery of L-asparaginase.
Carrier erythrocytes were prepared to encapsulate L-asparaginase by a hypotonic dialysis process. Dogs received either intravenous or intraperitoneal injections of cells containing both L-asparaginase and the marker [3H]inulin. The route of administration had no effect on the circulating survival of carrier erythrocytes. For enzyme therapy, intraperitoneal injection of a large volume of cells is technically feasible. Cells circulate with a maximum of 30% of the cells reaching circulation 24 h after injection. Those cells reaching circulation have a 7-day half-life for the encapsulated enzyme L-asparaginase.
DeLoach
J R
JR
U.S. Department of Agriculture, Veterinary Toxicology and Entomology Research Laboratory, College Station, Texas 77841.
Andrews
K
K
Satterfield
W
W
Keeling
M
M
D000818:Animals; D001215:Asparaginase / Q000008:administration & dosage*; D001709:Biotechnology; D004285:Dogs; D004905:Erythrocyte Aging; D004912:Erythrocytes; D006207:Half-Life; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D014677:Pharmaceutical Vehicles
D014677:Pharmaceutical Vehicles; D001215:Asparaginase