Electrochemical detection of the 3,5-dinitrobenzoyl derivative of digoxin by high-performance liquid chromatography.
Electrochemical detection of 3,5-dinitrobenzoyl derivatives of digoxin and its metabolites following high-performance liquid chromatography is reported. Partial resolution of derivatized digoxin and dihydrodigoxin was obtained using a Spherisorb ODS II analytical column. Both single- and dual-electrode detection were investigated and a maximum sensitivity equivalent to 0.39 ng of digoxin was found with the dual-electrode method. This system has the necessary sensitivity and selectivity for development into a therapeutic monitoring assay method.
Embree
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L
Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, Canada.
McErlane
K M
KM
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D004077:Digoxin / Q000031:analogs & derivatives* / Q000097:blood*; D004136:Dinitrobenzenes; D004563:Electrochemistry; D004566:Electrodes; D006801:Humans; D008991:Monitoring, Physiologic; D013056:Spectrophotometry, Ultraviolet
D004136:Dinitrobenzenes; C000967:dihydrodigoxin; D004077:Digoxin