Prediction of the resistance of human tumors to adriamycin by chemosensitivity tests and DNA analysis of the multidrug resistance gene.
In order to predict natural resistance to Adriamycin (ADM), the amplification of multidrug resistance gene 1 (MDR1) was investigated in 50 human cancer specimens using Southern blot analysis. Genomic DNA was extracted from both human solid tumors and adjacent normal tissues for the analysis. MDR1 gene amplification was not observed in any of the patients tested, including 5 patients in whom ADM was not clinically effective. On the other hand, chemosensitivity tests performed on the tumor cells of these 5 patients indicated resistance to ADM. Our results therefore indicate that MDR1 gene amplification is rarely seen among clinical samples and that conventional chemosensitivity tests might be more useful for the prediction of ADM resistance in cancer patients than the analysis of MDR1 gene amplification.
Kim
R
R
Department of Surgery, Hiroshima University, Japan.
Saeki
T
T
Takagami
S
S
Kirihara
Y
Y
Jinushi
K
K
Nishiyama
M
M
Niimoto
M
M
Hattori
T
T
Okada
K
K
D015139:Blotting, Southern; D002277:Carcinoma / Q000188:drug therapy* / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics; D004273:DNA, Neoplasm / Q000032:analysis*; D004317:Doxorubicin / Q000627:therapeutic use*; D004351:Drug Resistance / Q000235:genetics; D005784:Gene Amplification / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D009693:Nucleic Acid Hybridization; D009857:Oncogenes / Q000235:genetics*; D011336:Probability; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D004317:Doxorubicin