Immunoreactive growth hormone (GH) secretion by human lymphocytes: augmented release by exogenous GH.
Peripheral blood mononuclear cells (PBMCs) from normal adults secreted small amounts of human growth hormone (GH; 0.2-0.6 pg/10(5) cells/7 days culture) as measured by a highly sensitive enzyme immunoassay. Stimulation of PBMCs with phytohemagglutinin (PHA) consistently showed a 4-6 fold increase in GH secretion. Transformed B-lymphocytes by Epstein-Barr virus also secreted GH (0.8-4.8 pg/5 x 10(4) cells/7 days culture). GH secreted by lymphocytes comigrated with pituitary GH on an Ultrogel AcA44 column. Addition of GH during the culture augmented endogenous GH secretion from PHA-stimulated PBMCs. GH-releasing hormone and a somatostatin analogue, SMS 201-995, did not affect GH secretion from non-stimulated and PHA-stimulated PBMCs. These findings suggest that both T and B lymphocytes secrete immunoreactive GH in a different manner from that in the anterior pituitary.
Hattori
N
N
Department of Internal Medicine, Kyoto University Faculty of Medicine, Japan.
Shimatsu
A
A
Sugita
M
M
Kumagai
S
S
Imura
H
H
D000328:Adult; D001402:B-Lymphocytes / Q000378:metabolism; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D013006:Growth Hormone / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000378:metabolism
D010835:Phytohemagglutinins; D013006:Growth Hormone