Isolation and characterization of bacteriophage PM2 from Aeromonas hydrophila.
PM2 is an Aeromonas-specific bacteriophage isolated on A. hydrophila strain AH-3. The bacteriophage receptor for this phage was found to be the lipopolysaccharide (LPS), specifically a low-molecular weight LPS fraction (LPS-core oligosaccharides). Mutants resistant to this phage were isolated and found to be devoid of LPS O-antigen and altered in the LPS-core. No other outer-membrane (OM) molecules appeared to be involved in phage binding.
Merino
S
S
Departamento de Microbiología, Universidad de Barcelona, Spain.
Camprubi
S
S
Tomás
J M
JM
D000333:Aeromonas / Q000378:metabolism; D001435:Bacteriophages / Q000302:isolation & purification* / Q000378:metabolism / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D008070:Lipopolysaccharides / Q000032:analysis / Q000378:metabolism*; D008854:Microscopy, Electron; D009154:Mutation; D011991:Receptors, Virus / Q000032:analysis / Q000378:metabolism*; D013696:Temperature; D010948:Viral Plaque Assay
D008070:Lipopolysaccharides; D011991:Receptors, Virus