Excision of cytosine hydrates from Z-DNA.
Ultraviolet irradiation of DNA produces cytosine hydrate, released as a free base by E. coli endonuclease III. Cytosine hydrate excision was investigated by assaying photoproduct release from cytosine-radiolabeled, irradiated poly(dG-dC):poly(dG-dC). Conformational shifts between B-DNA and Z-DNA were affected by heating the polymer in either nickel chloride or cobaltous chloride, and were determined by circular dichroism. Rates of enzymic cytosine hydrate release did not differ between the different substrate conformations. Irradiation of left-handed poly(dG-dC):poly(dG-dC) resulted in cytosine hydrate formation. Therefore, neither formation nor enzymic excision of ultraviolet-induced cytosine hydrates are substantially affected by these DNA conformational states.
Duker
N J
NJ
Department of Pathology, Temple University School of Medicine, Philadelphia, PA 19140.
Weems
K M
KM
D002942:Circular Dichroism; D003596:Cytosine / Q000378:metabolism*; D004247:DNA / Q000378:metabolism / Q000528:radiation effects*; D043224:Deoxyribonuclease (Pyrimidine Dimer); D004706:Endodeoxyribonucleases / Q000378:metabolism*; D006868:Hydrolysis; D009690:Nucleic Acid Conformation; D014466:Ultraviolet Rays
D003596:Cytosine; D004247:DNA; D004706:Endodeoxyribonucleases; D043224:Deoxyribonuclease (Pyrimidine Dimer)