title: Abnormal calcium handling by platelets of spontaneously hypertensive rats.
abstract: There is controversy as to whether platelet intracellular free calcium ([Ca2+]i) is increased in spontaneously hypertensive rats (SHR) as compared with Wistar-Kyoto (WKY) rats. Discrepant results may be due to methodological problems including platelet activation during the collection process and leakage of intracellular dye used for [Ca2+]i measurement. To provide further insight into this problem, [Ca2+]i was estimated in fura-2-loaded platelets isolated from eight SHR and seven WKY rats at 12-14 weeks of age by using a two-syringe blood collection method and a correction method for fura-2 leakage. Basal [Ca2+]i was higher in SHR than in WKY rats (61.6 +/- 5.6 vs. 54.0 +/- 3.9 nM, p less than 0.02). However, the difference disappeared when a correction for fura-2 leakage was not used (109.7 +/- 18.4 vs. 94.9 +/- 9.2 nM, p less than 0.1). Thus, differences in [Ca2+]i between SHR and WKY rats may be obscured if dye leakage from platelets is not taken into account. Thrombin (0.1 units/ml) induced a rise in [Ca2+]i that was greater in SHR than WKY rats, both in the presence (491.4 +/- 31.6 vs. 377.5 +/- 21.7 nM, p less than 0.002) and absence (264.9 +/- 33.6 vs. 228.2 +/- 30.1 nM, p less than 0.05) of calcium in the media. These results indicate that thrombin-stimulated calcium influx as well as discharge of calcium from intracellular stores is increased in SHR platelets. Thus, under both basal and stimulated conditions, platelet calcium handling is abnormal in the SHR.
authors.affiliation: Division of Nephrology and Hypertension, Oregon Health Sciences University, Portland 97201.
authors.forename: T
authors.initials: T
authors.lastname: Oshima
authors.forename: E W
authors.initials: EW
authors.lastname: Young
authors.forename: R D
authors.initials: RD
authors.lastname: Bukoski
authors.forename: D A
authors.initials: DA
authors.lastname: McCarron
mesh_terms: D000634:Aminoquinolines; D000818:Animals; D001572:Benzofurans; D001792:Blood Platelets / Q000378:metabolism*; D001794:Blood Pressure; D002118:Calcium / Q000378:metabolism*; D005456:Fluorescent Dyes; D016257:Fura-2; D006973:Hypertension / Q000097:blood* / Q000503:physiopathology; D008297:Male; D015539:Platelet Activation; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D013917:Thrombin / Q000494:pharmacology
chemical_list: D000634:Aminoquinolines; D001572:Benzofurans; D005456:Fluorescent Dyes; D013917:Thrombin; C035739:Quin2; D002118:Calcium; D016257:Fura-2