title: Comparison of total serum lipids measured by two methods.
abstract: To accurately assess vitamin E status, the ratio of serum vitamin E to total serum lipids is required. We compared two methods of measuring total serum lipids: (a) the calculated sum of measured cholesterol, triglycerides, and phospholipids, and (b) the colorimetric method. Over the entire range of total lipid concentrations (151-1,728 mg/dl), there was an excellent correlation between methods (r = 0.930; p less than 0.001). No significant differences between measurements by these methods were found over the entire range and between 0 and 1,000 mg/dl; however, between 1,000 and 1,728 mg/dl, the measured total lipid concentration was higher (p = 0.023) than the added total lipid measurements. Despite this discrepancy, the methods appear comparable for clinical use in assessing vitamin E/total lipid status; the measured total lipids would be the preferred method because of the low cost and ease of performance of the test.
authors.affiliation: Division of Pediatric Gastroenterology and Nutrition, Children's Hospital Research Foundation, Cincinnati, Ohio.
authors.forename: J E
authors.initials: JE
authors.lastname: Heubi
authors.forename: R J
authors.initials: RJ
authors.lastname: Sokol
authors.forename: C A
authors.initials: CA
authors.lastname: McGraw
mesh_terms: D000328:Adult; D001774:Blood Chemical Analysis / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D002779:Cholestasis / Q000097:blood; D002784:Cholesterol / Q000097:blood; D003124:Colorimetry; D006801:Humans; D008055:Lipids / Q000097:blood*; D009752:Nutritional Status; D010743:Phospholipids / Q000097:blood; D014280:Triglycerides / Q000097:blood; D014810:Vitamin E / Q000097:blood; D014811:Vitamin E Deficiency / Q000097:blood
chemical_list: D008055:Lipids; D010743:Phospholipids; D014280:Triglycerides; D014810:Vitamin E; D002784:Cholesterol