[Cryopreservation of mouse strains by ultrarapid freezing].
Two-cell mouse embryos from four different inbred strains (BALB/c, C3H/He, C57BL/6 and DBA/2) and one closed colony (Slc:ICR) were frozen by direct placement into liquid nitrogen after a 10-15 sec exposure to a highly concentrated solution (DAP 213: 2 M dimethyl sulphoxide, 1 M acetamide, 3 M propylene glycol in PB 1), and later thawed in a 37 degrees C waterbath. The percentages of morphologically normal embryos were 80.7-92.6% on thawing. Morphologically normal embryos were then transferred to the oviducts of pseudopregnant recipients, and 7.4-60.0% of the embryos developed into normal young (BALB/c; 34.3%, C3H/He; 30.6%, C57BL/6; 60.0%, DBA/2; 7.4%, and Slc:ICR; 24.3%).
Nakagata
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Division of Pathology, Juntendo University School of Medicine, Tokyo, Japan.
D000818:Animals; D015925:Cryopreservation / Q000379:methods; D004624:Embryo Transfer; D004622:Embryo, Mammalian; D005260:Female; D005328:Fetal Viability; D005615:Freezing; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008813:Mice, Inbred ICR; D011247:Pregnancy