Morphological transformation and catalase activity of Syrian hamster embryo cells treated with hepatic peroxisome proliferators, TPA and nickel sulphate.
The abilities of the hepatic peroxisome proliferators (HPPs) clofibrate, di(2-ethylhexyl)phthalate (DEHP), mono(2-ethylhexyl)-phthalate (MEHP), 2,4-dichlorophenoxy acetic acid (2,4-D), 2,4,5-trichlorophenoxy acetic acid (2,4,5-T) and tiadenol to induce morphological transformation and to increase the catalase activity of Syrian hamster embryo (SHE) cells were studied. DEHP, MEHP, clofibrate and tiadenol induced morphological transformation of SHE cells and increased the catalase activity. DEHP was more potent than clofibrate and tiadenol in both inducing catalase and morphological transformation, while MEHP seemed more potent than DEHP in inducing catalase, but not morphological transformation, 2,4,5-T and 2,4-D did not induce morphological transformation, but 2,4,5-T was more potent than clofibrate in increasing the catalase activity. These results show that several HPPs induce morphological transformation of SHE cells and an increase in the catalase activity. There is, however, no direct connection between these two parameters, as seen from the results of 2,4,5-T. The tumor promoter TPA, and the metal salt nickel sulphate, induced morphological transformation of SHE cells without any appreciable increase in the catalase activity. These results further corroborate the dissociation between induction of morphological transformation and the increase in catalase activity.
Mikalsen
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Institute for Cancer Research, Norwegian Radium Hospital, Oslo.
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D015085:2,4,5-Trichlorophenoxyacetic Acid / Q000494:pharmacology*; D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology*; D000818:Animals; D002374:Catalase / Q000378:metabolism*; D002478:Cells, Cultured; D002994:Clofibrate / Q000494:pharmacology*; D006224:Cricetinae; D004051:Diethylhexyl Phthalate / Q000494:pharmacology* / Q000031:analogs & derivatives; D004622:Embryo, Mammalian / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D005233:Fatty Alcohols / Q000494:pharmacology*; D000960:Hypolipidemic Agents / Q000494:pharmacology*; D009532:Nickel / Q000494:pharmacology*; D010795:Phthalic Acids / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D005233:Fatty Alcohols; D000960:Hypolipidemic Agents; D010795:Phthalic Acids; C004568:tiadenol; D015084:2,4-Dichlorophenoxyacetic Acid; C029938:nickel sulfate; D009532:Nickel; D015085:2,4,5-Trichlorophenoxyacetic Acid; D004051:Diethylhexyl Phthalate; D002374:Catalase; C016599:mono-(2-ethylhexyl)phthalate; D002994:Clofibrate; D013755:Tetradecanoylphorbol Acetate