title: Laser desorption electron impact: application to a study of the mechanism of conjugation of glutathione and cyclophosphamide.
abstract: Toward the objective of producing ion radical species from involatile and thermally labile samples, we have combined laser desorption of neutral molecules with electron impact ionization on a time-of-flight mass analyzer with a delayed draw-out pulse. The analytical capabilities of this method are tested in the analysis of isotope labels in the involatile product in a mechanistic study of both the chemical and the enzyme catalyzed reactions of cyclophosphamide with glutathione.
authors.affiliation: Department of Chemistry and Biochemistry, University of Maryland Baltimore County 21228.
authors.forename: Z M
authors.initials: ZM
authors.lastname: Yuan
authors.forename: C
authors.initials: C
authors.lastname: Fenselau
authors.forename: D M
authors.initials: DM
authors.lastname: Dulik
authors.forename: W
authors.initials: W
authors.lastname: Martin
authors.forename: W B
authors.initials: WB
authors.lastname: Emary
authors.forename: R B
authors.initials: RB
authors.lastname: Brundrett
authors.forename: O M
authors.initials: OM
authors.lastname: Colvin
authors.forename: R J
authors.initials: RJ
authors.lastname: Cotter
mesh_terms: D000818:Animals; D003520:Cyclophosphamide / Q000378:metabolism; D003903:Deuterium; D004583:Electrons; D005978:Glutathione / Q000378:metabolism; D007834:Lasers; D013058:Mass Spectrometry / Q000379:methods; D011817:Rabbits; D013057:Spectrum Analysis / Q000379:methods
chemical_list: D003520:Cyclophosphamide; D003903:Deuterium; D005978:Glutathione