title: Synthesis of porcine brain natriuretic peptide-32 using silver tetrafluoroborate as a new deprotecting reagent of the S-trimethylacetamidomethyl group.
abstract: Silver tetrafluoroborate (AgBF4) in trifluoroacetic acid (TFA) has been found to cleave the S-trimethylacetamidomethyl (Tacm) group or the S-acetamidomethyl (Acm) group without affecting other functional groups in the peptide chain. Newly isolated porcine brain natriuretic peptide-32 (pBNP-32) was synthesized using AgBF4 and Cys(Tacm) derivatives. The synthetic pBNP-32 had the highest chick rectum relaxant activity among the ANP-BNP families.
authors.affiliation: Department of Medicinal Chemistry, Kyoto Pharmaceutical University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yoshida
authors.forename: K
authors.initials: K
authors.lastname: Akaji
authors.forename: T
authors.initials: T
authors.lastname: Tatsumi
authors.forename: S
authors.initials: S
authors.lastname: Iinuma
authors.forename: Y
authors.initials: Y
authors.lastname: Fujiwara
authors.forename: T
authors.initials: T
authors.lastname: Kimura
authors.forename: Y
authors.initials: Y
authors.lastname: Kiso
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001881:Borates; D001923:Brain Chemistry; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D020097:Natriuretic Peptide, Brain; D009419:Nerve Tissue Proteins / Q000138:chemical synthesis*; D012834:Silver; D018030:Silver Compounds; D013552:Swine
chemical_list: D001881:Borates; D007202:Indicators and Reagents; D009419:Nerve Tissue Proteins; D018030:Silver Compounds; D020097:Natriuretic Peptide, Brain; C064097:silver tetrafluoroborate; D012834:Silver