title: A 36-kDa mitochondrial protein is responsible for cyanide-resistant respiration in Hansenula anomala.
abstract: Antimycin A-dependent induction of cyanide-resistant respiration in Hansenula anomala was reversibly blocked by carbonylcyanide-m-chlorophenylhydrazone (CCCP). When the cells were pulse-labeled with [35S]methionine in the presence of both antimycin A and CCCP, the radioactivity was incorporated into a 39 kDa mitochondrial protein. Upon removal of CCCP, this protein was processed into a 36 kDa form. The increase in the 36 kDa protein completely paralleled that in cyanide-resistant respiration activity, suggesting that the 39 kDa protein is the precursor of the 36 kDa protein, which is responsible for cyanide-resistant respiration.
authors.affiliation: Department of Biochemistry, Niigata College of Pharmacy, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Minagawa
authors.forename: S
authors.initials: S
authors.lastname: Sakajo
authors.forename: T
authors.initials: T
authors.lastname: Komiyama
authors.forename: A
authors.initials: A
authors.lastname: Yoshimoto
mesh_terms: D000968:Antimycin A / Q000494:pharmacology; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology*; D005656:Fungal Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008715:Methionine / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D008970:Molecular Weight; D009570:Nitriles / Q000494:pharmacology*; D010101:Oxygen Consumption / Q000187:drug effects*; D010843:Pichia / Q000378:metabolism*; D004718:Saccharomycetales / Q000378:metabolism*; D013104:Spheroplasts / Q000187:drug effects / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D009570:Nitriles; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D000968:Antimycin A; D008715:Methionine