title: Direct DNA sequencing of PCR amplified genomic DNA by the Maxam-Gilbert method.
abstract: We present a method to directly sequence PCR amplification products utilizing the chemical method of Maxam and Gilbert. This procedure yields clearly readable DNA sequences of 100-400 base pairs in length derived from human genomic DNA in four days' time.
authors.affiliation: Yale University School of Medicine, Department of Human Genetics, New Haven, CT 06510.
authors.forename: T
authors.initials: T
authors.lastname: Tahara
authors.forename: J P
authors.initials: JP
authors.lastname: Kraus
authors.forename: L E
authors.initials: LE
authors.lastname: Rosenberg
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000302:isolation & purification*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction
chemical_list: D004247:DNA