Anal submucosal injection: a new route for drug administration in pelvic malignancies. III. Misonidazole distribution in serum, uterus and vagina: an experimental study.
The anal and oral administration routes were compared in 40 rats to study the distribution of misonidazole (MIS), a radiation sensitizer, in the serum, uterus and vagina. 14C-labelled MIS was administered in a dose of 0.2 ml water/100 g body weight containing 1 microCi MIS. The dose was given orally in 20 rats and was injected in the anal submucosa in another 20 rats. Animals were then sacrificed after 15, 30, 60 or 120 min or after 24 h. Serum samples was taken at the time of sacrifice; organs were dissected and radioactivity was determined in each by the internal standard method. The study has shown that the highest drug concentration in uterus and vagina relative to serum was achieved by the anal submucosal route. They showed a drug concentration of 10 and 8 times, respectively, of the serum level after 15 min in contrast to oral administration, which in the same period of time produced a drug concentration of 1/5 and 1/4 the serum level. The anal route thus offers an adequate channel for MIS administration to promote radiation responsiveness in cancer of uterus and vagina.
Shafik
A
A
Department of Surgery, Faculty of Medicine, Cairo University, Egypt.
el-Desouky
G
G
D000284:Administration, Oral; D000285:Administration, Rectal; D000818:Animals; D004333:Drug Administration Routes; D005260:Female; D007267:Injections / Q000379:methods*; D008920:Misonidazole / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics; D010386:Pelvic Neoplasms / Q000188:drug therapy*; D051381:Rats; D014018:Tissue Distribution; D014599:Uterus / Q000378:metabolism; D014621:Vagina / Q000378:metabolism
D008920:Misonidazole