Enzyme-linked immunosorbent assays for determination of plasma aldosterone using highly specific polyclonal antibodies.
Two enzyme-linked immunosorbent assays were established and compared for the estimation of plasma aldosterone. In the first method immobilized aldosterone-protein complexes on the ELISA plates compete with aldosterone to be determined for the binding of certain amount of anti-aldosterone antibodies. The sensitivity of this method depends on the protein carrier used to conjugate with aldosterone. In the second method, anti-aldosterone antibodies adsorbed on ELISA plates compete for binding of known amount of the enzyme-labeled aldosterone and aldosterone to be determined. The highly specific rabbit anti-aldosterone antibodies were obtained by injection of aldosterone-oxime thyroglobulin. The detection limit of aldosterone in both methods ranged between 2-20 pg. The proposed assays are suitable for the determination of aldosterone in biological fluids compared with other reported ELISA assays, as well as with RIA.
Schwartz
F
F
Department of Biotechnology, Faculty of Life Sciences, Tel-Aviv University, Israel.
Hadas
E
E
Harnik
M
M
Solomon
B
B
D000450:Aldosterone / Q000031:analogs & derivatives / Q000097:blood* / Q000276:immunology / Q000302:isolation & purification; D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity / Q000276:immunology; D001667:Binding, Competitive; D003429:Cross Reactions / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D008297:Male; D011817:Rabbits; D015203:Reproducibility of Results
D000450:Aldosterone