title: Enzyme-linked immunosorbent assays for determination of plasma aldosterone using highly specific polyclonal antibodies.
abstract: Two enzyme-linked immunosorbent assays were established and compared for the estimation of plasma aldosterone. In the first method immobilized aldosterone-protein complexes on the ELISA plates compete with aldosterone to be determined for the binding of certain amount of anti-aldosterone antibodies. The sensitivity of this method depends on the protein carrier used to conjugate with aldosterone. In the second method, anti-aldosterone antibodies adsorbed on ELISA plates compete for binding of known amount of the enzyme-labeled aldosterone and aldosterone to be determined. The highly specific rabbit anti-aldosterone antibodies were obtained by injection of aldosterone-oxime thyroglobulin. The detection limit of aldosterone in both methods ranged between 2-20 pg. The proposed assays are suitable for the determination of aldosterone in biological fluids compared with other reported ELISA assays, as well as with RIA.
authors.affiliation: Department of Biotechnology, Faculty of Life Sciences, Tel-Aviv University, Israel.
authors.forename: F
authors.initials: F
authors.lastname: Schwartz
authors.forename: E
authors.initials: E
authors.lastname: Hadas
authors.forename: M
authors.initials: M
authors.lastname: Harnik
authors.forename: B
authors.initials: B
authors.lastname: Solomon
mesh_terms: D000450:Aldosterone / Q000031:analogs & derivatives / Q000097:blood* / Q000276:immunology / Q000302:isolation & purification; D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity / Q000276:immunology; D001667:Binding, Competitive; D003429:Cross Reactions / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D008297:Male; D011817:Rabbits; D015203:Reproducibility of Results
chemical_list: D000450:Aldosterone