title: tert-Butyl hydroperoxide-induced injury in isolated rat hepatocytes: a model for studying anti-hepatotoxic crude drugs.
abstract: Tert-butyl hydroperoxide induces in freshly isolated rat hepatocytes malonaldehyde formation and lacticodehydrogenase and aspartate amino-transferase leakage. This model, when adapted to crude extracts, permits the demonstration of both anti-lipoperoxidant and antihepatotoxic activity of reference products like quercetin and silymarin and plant extracts like Rosmarinus officinalis and Eschscholzia californica.
authors.affiliation: Centre des Sciences de l'Environnement, Université de Metz, France.
authors.forename: M
authors.initials: M
authors.lastname: Joyeux
authors.forename: A
authors.initials: A
authors.lastname: Rolland
authors.forename: J
authors.initials: J
authors.lastname: Fleurentin
authors.forename: F
authors.initials: F
authors.lastname: Mortier
authors.forename: P
authors.initials: P
authors.lastname: Dorfman
mesh_terms: D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D019684:Magnoliopsida / Q000032:analysis; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010545:Peroxides / Q000633:toxicity*; D010936:Plant Extracts / Q000494:pharmacology; D011398:Promethazine / Q000494:pharmacology; D011794:Quercetin / Q000494:pharmacology; D051381:Rats; D012838:Silymarin / Q000494:pharmacology; D020122:tert-Butylhydroperoxide
chemical_list: D010545:Peroxides; D010936:Plant Extracts; D012838:Silymarin; D008315:Malondialdehyde; D020122:tert-Butylhydroperoxide; D011794:Quercetin; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D011398:Promethazine