Antiproliferative action of retinoic acid in cultured human brain tumour cells Gl-As-14(S).
We have employed human Gl-As-14(S) brain tumour cell line to study antiproliferative action of retinoic acid (RA). RA (20 microM) caused a time-dependent, dose-dependent, cell seeding density-dependent reduction in cell proliferation in liquid medium and inhibited growth in agar. Growth inhibitory effects of RA were also affected by the concentration of fetal bovine serum (PBS) in the medium. All these effects could be reversed within 48 h after removal of RA from the growth medium. RA-treated cells also displayed reduced concanavalin A (Con A) binding ability by microhemadsorption technique. The results demonstrated that RA can suppress in this tumour cell line the expression of some properties frequently associated with transformed cells.
Mukherjee
P
P
Department of Tissue Culture, Chittaranjan National Cancer Institute, Calcutta, India.
Das
S K
SK
D000970:Antineoplastic Agents; D001932:Brain Neoplasms / Q000473:pathology; D002449:Cell Aggregation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D006382:Hemadsorption / Q000187:drug effects; D006801:Humans; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000378:metabolism
D000970:Antineoplastic Agents; D003470:Culture Media; D003208:Concanavalin A; D014212:Tretinoin