title: Identification and nucleotide sequence of the Acinetobacter calcoaceticus encoded trpE gene.
abstract: The trpE gene from Acinetobacter calcoaceticus encoding the anthranilate synthase component I was cloned, identified by deletion analysis and sequenced. It encodes a predicted polypeptide of 497 amino acids with a calculated molecular weight of 55,323. Its primary structure shows 49% identical amino acids with the enzyme from Clostridium thermocellum, 45% with that of Thermus thermophilus and only 35% with that of Escherichia coli. The codon usage of the trpE genes encoding the most homologous enzymes differs greatly indicating selection for amino acid maintainance. The homologies are clustered in the C-terminal 200 amino acids of the sequences indicating that this part is important for enzymic activity.
authors.affiliation: Lehrstuhl für Mikrobiologie, Friedrich-Alexander Universität Erlangen-Nürnberg, Federal Republic of Germany.
authors.forename: G
authors.initials: G
authors.lastname: Haspel
authors.forename: M
authors.initials: M
authors.lastname: Hunger
authors.forename: R
authors.initials: R
authors.lastname: Schmucker
authors.forename: W
authors.initials: W
authors.lastname: Hillen
mesh_terms: D000150:Acinetobacter / Q000201:enzymology / Q000235:genetics*; D000878:Anthranilate Synthase / Q000235:genetics*; D001483:Base Sequence; D004252:DNA Mutational Analysis; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012689:Sequence Homology, Nucleic Acid
chemical_list: D000878:Anthranilate Synthase