Absence of correlations between radiosensitivities of human T-lymphocytes in G0 and skin fibroblasts in log phase.
Dose-survival curves were obtained for matched samples of peripheral T-lymphocytes and skin fibroblasts from a total of 22 patients who underwent various surgical procedures using loss of colony-forming ability as the end point. The results showed that the mean D10 (dose required to kill 90% of cells) +/- SD was 3.58 +/- 0.21 Gy for T-lymphocytes irradiated in G0 and 3.19 +/- 0.37 Gy for skin fibroblasts irradiated in log phase. The coefficients of variation were found to be 6 and 11%, respectively. Contrary to the expectation, regression analysis of D10 values for the two types of cells revealed no significant correlations. The absence of correlation most probably derives from the fact that the apparent interindividual variability of dose-survival curves is caused primarily by random experimental fluctuations at least in the case of lymphocytes. Possible reasons for the greater variability observed in the fibroblast assay are discussed.
Kushiro
J
J
Department of Radiobiology, Radiation Effects Research Foundation, Hiroshima, Japan.
Nakamura
N
N
Kyoizumi
S
S
Nishiki
M
M
Dohi
K
K
Akiyama
M
M
D000328:Adult; D000368:Aged; D002453:Cell Cycle; D002470:Cell Survival / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005347:Fibroblasts / Q000166:cytology* / Q000528:radiation effects; D006801:Humans; D007399:Interphase; D008297:Male; D008875:Middle Aged; D011836:Radiation Tolerance; D012867:Skin / Q000166:cytology; D013601:T-Lymphocytes / Q000166:cytology* / Q000528:radiation effects