Assignment of the human gene for histidine-rich glycoprotein to chromosome 3.
Histidine-rich glycoprotein (HRG) is a monomeric plasma glycoprotein involved in the modulation of coagulation and fibrinolysis. Using Southern analysis of human-rodent somatic cell hybrid DNA with a human HRG-specific cDNA probe, the HRG gene was assigned to chromosome 3. One hybrid that was known to contain only a segment of chromosome 3 also reacted positively with the HRG probe. Hybridization analysis with a set of chromosome 3-specific probes showed that the segment of chromosome 3 present in this hybrid is missing the region pter-p14, which indicates that HRG is not located in this region. No restriction fragment length polymorphisms were detected for HRG with 10 commonly used restriction enzymes.
van den Berg
E A
EA
Gaubius Institute, Leiden, The Netherlands.
le Clercq
E
E
Kluft
C
C
Koide
T
T
van der Zee
A
A
Oldenburg
M
M
Wijnen
J T
JT
Meera Khan
P
P
D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D005796:Genes; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D011506:Proteins / Q000235:genetics*; D000099053:Histidine-Rich Glycoprotein
D006023:Glycoproteins; D011506:Proteins; D000099053:Histidine-Rich Glycoprotein