title: Direct in vitro isolation of Trypanosoma brucei brucei and T. b. evansi from disease hosts with low parasitaemia.
abstract: Three stocks of Trypansoma brucei brucei and two stocks of T. b. evansi were isolated directly from host animals with a low parasitaemia. Cultures were initiated under axenic conditions and/or in co-cultivation with different feeder layer cells using a modified Eagle's MEM. Under axenic conditions, HAM's F-12, Iscove's modified Dulbecco medium and SFRE 199-2 medium were also suitable, whereas RPMI 1640, and Leibovitz L-15 medium were not. The method described was sensitive and detected parasites which were not evident in some cases on wet blood film or haematocrit centrifuge techniques.
authors.affiliation: Kenya Trypanosomiasis Research Institute, Kikuyu.
authors.forename: E
authors.initials: E
authors.lastname: Zweygarth
authors.forename: R
authors.initials: R
authors.lastname: Kaminsky
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D013552:Swine; D014346:Trypanosoma brucei brucei / Q000302:isolation & purification*; D014353:Trypanosomiasis, African / Q000469:parasitology / Q000662:veterinary*
chemical_list: D003470:Culture Media