title: Evaluation of a commercial microtiter system (MicroScan) using both frozen and freeze-dried panels for detection of high-level aminoglycoside resistance in Enterococcus spp.
abstract: The MicroScan system was compared with agar dilution screen plates for the detection of high-level aminoglycoside resistance in 182 enterococcal isolates. Both the frozen Gram-Positive Combo Type 2 and the freeze-dried Type 5 panels were evaluated. The specificity of both panels for the detection of streptomycin and gentamicin resistance was 100%. However, the sensitivities for the detection of gentamicin and streptomycin resistance were 84 and 31%, respectively, for the Type 2 panels and 90 and 41%, respectively, for the Type 5 panels. The sensitivities of these panels for the detection of enterococcal high-level aminoglycoside resistance are inadequate for routine use.
authors.affiliation: Department of Microbiology, University of Toronto, Ontario, Canada.
authors.forename: S A
authors.initials: SA
authors.lastname: Fuller
authors.forename: D E
authors.initials: DE
authors.lastname: Low
authors.forename: A E
authors.initials: AE
authors.lastname: Simor
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D005069:Evaluation Studies as Topic; D005612:Freeze Drying; D005615:Freezing; D005839:Gentamicins / Q000494:pharmacology; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D013291:Streptococcus / Q000187:drug effects* / Q000302:isolation & purification; D013307:Streptomycin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; D013307:Streptomycin