N-(9-acridinyl)-bromoacetamide--a powerful reagent for phase-transfer-catalyzed fluorescence labeling of carboxylic acids for liquid chromatography.
Phase-transfer-catalyzed esterification of various carboxylic acids has been carried out on a microanalytical scale with a new reagent, N-(9-acridinyl)-bromoacetamide, in aqueous/organic two-phase systems. The procedure gives esters showing very high fluorescence intensity in acid solution. The large Stokes' shift (greater than 120 nm) means that a low background can be achieved even when using a large emission bandwidth. These derivatives show very good chromatographic behavior on reversed-phase liquid chromatographic columns with the use of aqueous acetonitrile, containing 0.2% of phosphoric acid, as the mobile phase. Detection limits (S/N = 2) as low as 10 fmol can be obtained under optimal conditions.
Allenmark
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Laboratory of Microbiological Chemistry, University of Gothenburg, Sweden.
Chelminska-Bertilsson
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Thompson
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D000609:Aminoacridines; D001647:Bile Acids and Salts / Q000032:analysis; D002264:Carboxylic Acids / Q000032:analysis*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002853:Chromatography, Liquid / Q000379:methods; D004951:Esterification; D004988:Ethers, Cyclic / Q000032:analysis; D019319:Okadaic Acid; D013050:Spectrometry, Fluorescence
D000609:Aminoacridines; D001647:Bile Acids and Salts; D002264:Carboxylic Acids; D004988:Ethers, Cyclic; C064158:N-(9-acridinyl)bromoacetamide; D019319:Okadaic Acid