Multinuclear magnetic resonance studies of the 2Fe.2S* ferredoxin from Anabaena species strain PCC 7120. 2. Sequence-specific carbon-13 and nitrogen-15 resonance assignments of the oxidized form.
Multinuclear two-dimensional NMR techniques were used to assign nearly all diamagnetic 13C and 15N resonances of the plant-type 2Fe.2S* ferredoxin from Anabaena sp. strain PCC 7120. Since a 13C spin system directed strategy had been used to identify the 1H spin systems [Oh, B.-H., Westler, W. M., & Markley, J. L. (1989) J. Am. Chem. Soc. 111, 3083-3085], the sequence-specific 1H assignments [Oh, B.-H., & Markley, J. L. (1990) Biochemistry (first paper of three in this issue)] also provided sequence-specific 13C assignments. Several resonances from 1H-13C groups were assigned independently of the 1H assignments by considering the distances between these nuclei and the paramagnetic 2Fe.2S* center. A 13C-15N correlation data set was used to assign additional carbonyl carbons and to analyze overlapping regions of the 13C-13C correlation spectrum. Sequence-specific assignments of backbone and side-chain nitrogens were based on 1H-15N and 13C-15N correlations obtained from various two-dimensional NMR experiments.
Oh
B H
BH
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison 53706.
Mooberry
E S
ES
Markley
J L
JL
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D002247:Carbon Isotopes; D056890:Eukaryota / Q000032:analysis*; D005288:Ferredoxins / Q000032:analysis*; D007501:Iron / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes; D010449:Peptide Mapping; D011487:Protein Conformation
D001426:Bacterial Proteins; D002247:Carbon Isotopes; D005288:Ferredoxins; D009587:Nitrogen Isotopes; C064537:petF protein, Anabaena; D007501:Iron