[Synthesis of N-glycoproteins with a native type of carbohydrate-peptide bond].
Reaction of beta-oligoglycosylamines obtained from carbohydrate chains of N-glycoproteins (ovalbumin, ovomucoid, riboflavin-binding glycoprotein from hen egg white, and asialofetuin) with bovine serum albumin, lysozyme, and poly(L-Asp) in presence of water-soluble carbodiimide gave rise to a series of glycoconjugates, modelling natural N-glycoproteins. Carbohydrate-peptide bond was shown to be of N-glycosylamide type with participation of Asp and Glu residues. The method allows one to obtain synthetic N-glycoproteins from oligomannoside, complex and hybrid oligosaccharide chains, and may find application both in biochemistry and biotechnology for improvement of physico-chemical properties of unglycosylated proteins.
Likhosherstov
L M
LM
Piskarev
V E
VE
Derevitskaia
V A
VA
Kochetkov
N K
NK
D055598:Chemical Phenomena; D002621:Chemistry; D006023:Glycoproteins / Q000138:chemical synthesis*; D009844:Oligosaccharides; D010455:Peptides
D006023:Glycoproteins; D009844:Oligosaccharides; D010455:Peptides
Sintez N-glikoproteinov s prirodnym tipom uglevod-peptidnoĭ sviazi.