Contribution of monoamine oxidase(MAO) to the binding of tertiary basic drugs in isolated perfused rat lung.
The effect of tertiary basic drugs on mitochondrial MAO activity and the effect of MAO inhibitors (MAOIs) on basic drug accumulation in the isolated perfused rat lung were studied to clarify the role of MAO in drug binding to lung tissue. In the perfused lung preparation, the inhibition of MAO by basic drugs correlated well with their lipid solubilities and followed competitive kinetics. The inhibitory rank order (imipramine not equal to diphenhydramine greater than quinine greater than metoclopramide greater than procainamide) also correlated with their accumulation in the perfused lung. Moreover, MAOI treatment decreased the accumulation of basic drugs in the lung, and the potency of MAOIs to inhibit drug accumulation in the lung correlated with their MAO inhibitory activity. These results indicate that lung MAO has specific binding sites for basic drugs and may function as a drug reservoir.
Yoshida
H
H
Department of Transfusion Medicine, Kyoto University Hospital, Faculty of Medicine, Japan.
Okumura
K
K
Hori
R
R
D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D066298:In Vitro Techniques; D007700:Kinetics; D008168:Lung / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology; D008995:Monoamine Oxidase / Q000378:metabolism*; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D010627:Phenethylamines / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012995:Solubility
D008996:Monoamine Oxidase Inhibitors; D004364:Pharmaceutical Preparations; D010627:Phenethylamines; D008995:Monoamine Oxidase