Prevalence of tri- and tetraantennary glycans of human alpha 1-acid glycoprotein in release of macrophage inhibitor of interleukin-1 activity.
Based on the affinity for concanavalin A (Con A), human alpha 1-acid glycoprotein (AGP) can be separated by chromatography on Con A-Sepharose gel into three variants: Con A unreactive AGP, Con A weakly reactive AGP, and Con A strongly reactive AGP. When exposed to native AGP or to its glycan variants, murine peritoneal macrophages released a factor that inhibited the interleukin-1 (IL-1) proliferative activity as measured in terms of the thymocyte comitogenic assay. Con A unreactive AGP, which contains tri- and tetraantennary glycans and no biantennae, proved to be more effective than Con A weakly and Con A strongly reactive variants, which contain one and two diantennary glycans, respectively. The inhibitory effect was not a function of the negative charge related to the sialyl residues and was not mediated by the mannosyl-fucosyl receptor.
Bories
P N
PN
Laboratoire de Biochimie, Université Paris-Sud, Châtenay-Malabry, France.
Feger
J
J
Benbernou
N
N
Rouzeau
J D
JD
Agneray
J
J
Durand
G
G
D000818:Animals; D003208:Concanavalin A / Q000378:metabolism; D005643:Fucose / Q000302:isolation & purification / Q000502:physiology; D006031:Glycosylation; D006801:Humans; D007375:Interleukin-1 / Q000037:antagonists & inhibitors*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D019158:N-Acetylneuraminic Acid; D009961:Orosomucoid / Q000032:analysis* / Q000494:pharmacology; D011134:Polysaccharides / Q000302:isolation & purification*; D011499:Protein Processing, Post-Translational; D012794:Sialic Acids / Q000302:isolation & purification / Q000502:physiology; D013601:T-Lymphocytes / Q000187:drug effects
D007375:Interleukin-1; D009961:Orosomucoid; D011134:Polysaccharides; D012794:Sialic Acids; D003208:Concanavalin A; D005643:Fucose; D019158:N-Acetylneuraminic Acid