Inducible cytosolic enzyme activity for the production of nitrogen oxides from L-arginine in hepatocytes.
The in vivo conditions needed for the induction of nitrogen oxide synthesis by hepatocytes were determined. Hepatocytes obtained from rats injected with killed Corynebacterium parvum spontaneously produced NO2(-)+NO3- in culture and were found to contain cytosolic enzyme activity for nitrogen oxide synthesis. The enzyme activity required both L-arginine and NADPH, and was not found in hepatocytes obtained from normal rats or rats injected with lipopolysaccharide (LPS) alone. In contrast, nonparenchymal cells were stimulated to synthesize NO2(-)+NO3- by LPS. These results show the presence of inducible cytosolic enzyme activity for nitrogen oxide synthesis in hepatocytes, which is distinct from nonparenchymal cell NO. synthesis.
Billiar
T R
TR
Department of Neurobiology, Anatomy, and Cell Science, University of Pittsburgh, PA 15261.
Curran
R D
RD
Stuehr
D J
DJ
Stadler
J
J
Simmons
R L
RL
Murray
S A
SA
D000818:Animals; D001120:Arginine / Q000378:metabolism*; D002478:Cells, Cultured; D003600:Cytosol / Q000201:enzymology*; D004790:Enzyme Induction / Q000187:drug effects; D008025:Ligases / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D009249:NADP / Q000494:pharmacology; D009589:Nitrogen Oxides / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D008070:Lipopolysaccharides; D009589:Nitrogen Oxides; D009249:NADP; D001120:Arginine; D008025:Ligases