title: [Fluorescent method of determination of serum albumin in hyperbilirubinemia].
abstract: A procedure is developed for estimation of human blood serum albumin in hyperbilirubinemia using a fluorescent probe 9,10-dianiline-2-sulfoanthracene (K-33). Use of the probe N-phenyl-I-amino-8-sulfonaphthalene (ANS) led to underestimation of albumin in hyperbilirubinemia as bilirubin and other inhibitors prevented the ANS binding with blood serum albumin. Substitution of ANS for K-33 enabled to estimate the albumin concentration under conditions of the pathology accompanied by an increase of endogenous ligands content in blood serum. The rate of K-33 (15 mM) fluorescence in blood serum (diluted 400-fold, at pH 4.0) correlated with albumin concentration (r-0.955).
authors.forename: Iu I
authors.initials: IuI
authors.lastname: Miller
authors.forename: E G
authors.initials: EG
authors.lastname: Iushko
authors.forename: G E
authors.initials: GE
authors.lastname: Dobretsov
authors.forename: B M
authors.initials: BM
authors.lastname: Krasovitskiĭ
authors.forename: R K
authors.initials: RK
authors.lastname: Aĭdyraliev
mesh_terms: D005456:Fluorescent Dyes; D006801:Humans; D006932:Hyperbilirubinemia / Q000097:blood*; D012709:Serum Albumin / Q000032:analysis*; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D012709:Serum Albumin
vernacular_title: Fliuorestsentnyĭ metod opredeleniia al'bumina v syvorotke krovi pri giperbilirubinemii.