Very fast purification of ornithine decarboxylase with high yield from mouse kidney and generation of a monoclonal antibody.
Based on methods for ornithine-decarboxylase purification published previously we developed an improved procedure for purification of the enzyme from the kidneys of testosterone-treated NMRI mice. Advantages of the new procedure are, that inactivation of the enzyme during purification is largely reduced by fast methods for purification and by the use of proteinase inhibitors. That way we got pure ornithine decarboxylase within 60 h with a yield of about 70%. A part of the highly purified ornithine decarboxylase was used for the generation of monoclonal antibodies.
Kopitz
J
J
Physiologisch-Chemisches Institut, Universität Tübingen.
Adam
G
G
Bohley
P
P
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D007668:Kidney / Q000201:enzymology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009955:Ornithine Decarboxylase / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D051381:Rats; D013739:Testosterone / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D013739:Testosterone; D009955:Ornithine Decarboxylase