title: The specificity of the antibody response to internal antigens of Ascaris: heterogeneity in infected humans, and MHC (H-2) control of the repertoire in mice.
abstract: Children from an area of Africa endemic for the large roundworm of humans, Ascaris lumbricoides, were found to vary considerably in the specificity of their serum IgG response to the internal antigens of the parasite. This was particularly noticeable for responses to a 14-kD protein (ABA-1) of the parasite that has previously been shown to be the subject of a strong IgE antibody response in infected animals. The possibility that this heterogeneity in immune repertoire has a genetic basis was explored in inbred mice infected with Ascaris suum. This showed that no strain responded to all the potential antigens, that the recognition profiles of strains bearing independent haplotypes were unique, and only H-2-identical strains had responses of similar specificities. Major histocompatability complex (MHC) restriction was confirmed using H-2-congenic animals on BALB and B10 backgrounds, which responded according to their H-2 haplotype. It is likely, therefore, that it is the MHC which controls the repertoire to Ascaris antigens in infected people. If this is so, then there will be implications for immunopathology associated with ascariasis, and possibly also for resistance and susceptibility to infection.
authors.affiliation: Wellcome Laboratories for Experimental Parasitology, University of Glasgow, Bearsden, UK.
authors.forename: M W
authors.initials: MW
authors.lastname: Kennedy
authors.forename: L A
authors.initials: LA
authors.lastname: Tomlinson
authors.forename: E M
authors.initials: EM
authors.lastname: Fraser
authors.forename: J F
authors.initials: JF
authors.lastname: Christie
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis*; D000918:Antibody Specificity / Q000235:genetics*; D000947:Antigens, Helminth / Q000276:immunology*; D001196:Ascariasis / Q000276:immunology; D001200:Ascaris / Q000276:immunology*; D006183:H-2 Antigens / Q000502:physiology*; D006801:Humans; D051379:Mice; D008815:Mice, Inbred Strains
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D006183:H-2 Antigens