[Synthetic anabolic androgens in the fecal material of cattle: radioimmunological determination after purification by high performance liquid chromatography].
In answer to the mandatory control of the illegal use of anabolizing agents in meat-producing animals imposed by the EEC in farms, a method of analysis of faeces involving high performance liquid chromatography (HPLC) and radioimmunoassay (RIA) has been described. Four HPLC fractions were collected and submitted to corresponding RIA: 17 beta- and 17 alpha-trenbolone, 17 beta-nortestosterone, 17 alpha-nortestosterone and 17 alpha-methyltestosterone. The mean extraction and purification yield was estimated at 44 +/- 7% using tritiated 17 alpha-methyltestosterone as internal standard. Detection limits of the 3 hormones were estimated at 0.2-0.3 ng/g of faeces. About 50 samples can be analysed per week by this method.
Delahaut
P
P
Laboratoire d'hormonologie animale, Centre d'économie rurale, Marloie.
Dubois
M
M
Maghuin-Rogister
G
G
D045930:Anabolic Agents / Q000032:analysis* / Q000302:isolation & purification; D000818:Animals; D002417:Cattle / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005243:Feces / Q000032:analysis*; D008777:Methyltestosterone / Q000032:analysis / Q000302:isolation & purification; D009277:Nandrolone / Q000032:analysis / Q000302:isolation & purification; D011237:Predictive Value of Tests; D011863:Radioimmunoassay; D015203:Reproducibility of Results; D014204:Trenbolone Acetate / Q000032:analysis / Q000302:isolation & purification
D045930:Anabolic Agents; D009277:Nandrolone; D014204:Trenbolone Acetate; D008777:Methyltestosterone
Androgènes anabolisants de synthèse dans les matières fécales des bovins: dosage radio-immunologique après purification par chromatographie liquide à haute performance.