Enhanced production of interleukin-1 and tumor necrosis factor alpha by cultured peripheral blood monocytes from patients with scleroderma.
Cultured peripheral blood mononuclear cells from 20 patients with scleroderma were shown to produce high levels of interleukin-1 alpha, interleukin-1 beta, and tumor necrosis factor alpha. There were positive correlations between the production of these 3 molecules. Purified monocytes from these patients produced much more tumor necrosis factor alpha than did those from normal subjects, even without lipopolysaccharide stimulation. These results show the hyperactivity of monocytes from scleroderma patients.
Umehara
H
H
Second Department of Internal Medicine, Kyoto University Medical School, Japan.
Kumagai
S
S
Murakami
M
M
Suginoshita
T
T
Tanaka
K
K
Hashida
S
S
Ishikawa
E
E
Imura
H
H
D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015592:Immunoradiometric Assay; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000097:blood; D008070:Lipopolysaccharides / Q000494:pharmacology; D009000:Monocytes / Q000378:metabolism*; D012595:Scleroderma, Systemic / Q000097:blood*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D007375:Interleukin-1; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha