Effects of iron-binding proteins on in vitro uptake of 67Ga-citrate by tumor cells.
A comparative study of carrier-free 67Ga-citrate uptake by Ehrlich ascites tumor cells in the presence of lactoferrin, transferrin and ferritin has demonstrated that lactoferrin considerably increases the uptake of 67Ga, and that this increase seems to be determined by its iron-load. The other iron-binding proteins assayed have a null or negative effect. Their behavior in the presence of sodium citrate supports the concept of lactoferrin-binding by the cells as responsible for the uptake. The different behavior of 67Ga-citrate iron-binding protein complexes appears to support this hypothesis.
Anghileri
L J
LJ
Nuclear Medicine Service, Faculty of Medicine, University of Nancy, France.
Thouvenot
P
P
Brunotte
F
F
Crone Escanye
M C
MC
Robert
J
J
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000000981:diagnostic imaging / Q000378:metabolism*; D002951:Citrates / Q000493:pharmacokinetics*; D019343:Citric Acid; D005293:Ferritins / Q000494:pharmacology*; D005710:Gallium Radioisotopes; D066298:In Vitro Techniques; D007781:Lactoferrin / Q000494:pharmacology*; D007782:Lactoglobulins / Q000494:pharmacology*; D011877:Radionuclide Imaging; D014168:Transferrin / Q000494:pharmacology*
D002951:Citrates; D005710:Gallium Radioisotopes; D007782:Lactoglobulins; D014168:Transferrin; D019343:Citric Acid; D005293:Ferritins; D007781:Lactoferrin