Methods for the isolation and detection of etheno adducts in nucleotide pools in vivo following exposure to ethyl carbamate.
Cellular extraction and high-performance liquid chromatographic methods were developed for the isolation of etheno adducts from nucleotide pools formed in vivo following exposure to the chemical carcinogen ethyl carbamate. These techniques were employed to detect etheno adduct formation using BDF1 mice and rainbow trout (Salmo gairdneri) as test species following inter-peritoneal injection of the chemical. Ethenoadenine was detected in splenocyte nucleotide pools of mice after acute (24 h) exposure and chronic (two weeks) exposure. Several etheno adducts (i.e. ethenoadenine, etheno-AMP, etheno-ADP and etheno-ATP) were also detected in total spleen cell nucleotide pools of trout following acute ethyl carbamate exposure.
Mayernik
J A
JA
Program in Toxicology, Massachusetts Institute of Technology, Cambridge 02139.
Conner
M K
MK
Giam
C S
CS
D000225:Adenine / Q000031:analogs & derivatives / Q000302:isolation & purification; D000244:Adenosine Diphosphate / Q000031:analogs & derivatives* / Q000302:isolation & purification; D000249:Adenosine Monophosphate / Q000031:analogs & derivatives / Q000302:isolation & purification; D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004985:Ethenoadenosine Triphosphate / Q000302:isolation & purification; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009705:Nucleosides / Q000302:isolation & purification*; D013154:Spleen / Q000187:drug effects / Q000378:metabolism; D014337:Trout; D014520:Urethane / Q000494:pharmacology*
D002273:Carcinogens; D004985:Ethenoadenosine Triphosphate; D009705:Nucleosides; C004981:1,N(6)-ethenoadenine; C030368:etheno-AMP; C015195:1,N(6)-ethenoadenosine diphosphate; D014520:Urethane; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000225:Adenine