Rapid analysis of mouse-hamster hybrid cell lines by in situ hybridization.
In situ hybridization techniques for analyzing the murine DNA complement of mouse-hamster hybrid cells are described. Total genomic mouse DNA is labeled with biotin and hybridized without suppression to metaphase spreads from a mouse-hamster hybrid line containing the mouse fusion chromosome X12. Detection via fluorochrome-conjugated avidin reveals mouse chromosomal DNA with high sensitivity and permits the identification of both normal and aberrant murine chromosomes. Conversely, biotinylated total genomic DNA from a hybrid line can be used as a probe on normal mouse metaphase spreads if suppression techniques are employed, facilitating the analysis of mouse chromosomes present in the hybrid line.
Boyle
A L
AL
Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06510.
Lichter
P
P
Ward
D C
DC
D000818:Animals; D002875:Chromosomes; D006224:Cricetinae; D004247:DNA / Q000032:analysis; D006822:Hybrid Cells; D007621:Karyotyping; D051379:Mice; D009693:Nucleic Acid Hybridization; D012091:Repetitive Sequences, Nucleic Acid; D014960:X Chromosome
D004247:DNA