Retinoidal benzoic acids (arotenoids) and other retinoids inhibit in vitro transformation of epithelial cells.
Five retinoids were evaluated for their ability to inhibit N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced transformation of primary rat tracheal epithelial (RTE) cells in culture. Two retinoidal benzoic acids (arotenoids), Ro 13-6298 and Ro 13-7410, suppressed the transformation frequency by 80-90% at 330 pM; 330 pM retinoic acid (RA) suppressed the transformation frequency by 50%. Retinol and retinyl acetate were tested at 33 nM and found to be much less effective than RA at this concentration. In studies with all retinoids, the amount of inhibition of spontaneously occurring transformed epithelial foci was greater than the amount of inhibition of foci that arose in response to carcinogen treatment. A biological difference between carcinogen-induced and spontaneously arising foci is suggested by this result.
Mass
M J
MJ
Respiratory Carcinogenesis Group, U.S. Environmental Protection Agency, Research Triangle Park, NC 27711.
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001565:Benzoates / Q000494:pharmacology*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002478:Cells, Cultured; D004224:Diterpenes; D004305:Dose-Response Relationship, Drug; D004848:Epithelium / Q000187:drug effects / Q000473:pathology; D008297:Male; D008769:Methylnitronitrosoguanidine; D051381:Rats; D011916:Rats, Inbred F344; D012176:Retinoids / Q000494:pharmacology*; D000084562:Retinyl Esters; D014132:Trachea / Q000187:drug effects / Q000473:pathology; D014801:Vitamin A / Q000031:analogs & derivatives / Q000494:pharmacology
D000970:Antineoplastic Agents; D001565:Benzoates; D004224:Diterpenes; D012176:Retinoids; D000084562:Retinyl Esters; D014801:Vitamin A; D008769:Methylnitronitrosoguanidine; C009166:retinol acetate; C034814:ethyl-p-((E)-2-(5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2-naphthyl)-1-propenyl)benzoic acid; C038864:4-(2-(5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2-naphthalenyl)-1-propenyl)benzoic acid