Induction of spermidine/spermine N1-acetyltransferase activity in Chinese-hamster ovary cells by N1N11-bis(ethyl)norspermine (corrected) and related compounds.
Treatment of Chinese-hamster ovary (CHO) cells with N1N11-bis(ethyl)norspermine (BENSM) led to a very large increase in the activity of spermidine/spermine N1-acetyltransferase (SAT), which rose by about 600-fold within 48 h. Smaller, but still very large increases, were also produced in decreasing order of potency by 3,7,11,15,19-penta-azaheneicosane, N1N12-bis(ethyl)spermine and by N1N14-bis(ethyl)homospermine. The rise in acetyltransferase activity was due to an increase in enzyme protein, as indicated by immunoblotting using antibodies directed against rat liver SAT. There was an increase in the content of mRNA for SAT, indicating that BENSM regulates the level of enzyme protein partly by means of a change in transcription or stability of the mRNA. There was also a decreased rate of degradation of the protein in CHO cells trated with the drug. This may be due to the binding of BENSM, which is a competitive inhibitor of the enzyme with a Ki of 120 microM. Exposure to BENSM led to an increased conversion of spermidine into N1-acetylspermidine and putrescine, a rapid fall in the content of intracellular polyamines and the excretion from the cell of putrescine, N1-acetylspermidine and spermidine. When polyamine oxidase activity in the treated cells was blocked, increases in N1-acetylspermidine and N1-acetylspermine were much greater, and the formation of putrescine was prevented. These results indicate that the induction of SAT facilities the degradation of spermine and spermidine to putrescine and the subsequent excretion of putrescine from the cell. When the degradation of the N1-acetyl derivatives by polyamine oxidase is blocked, the cells excrete N1-acetylspermidine instead of putrescine. CHO cells also contained and excreted N8-acetylspermidine, but its synthesis was not increased in cells treated with BENSM, confirming data obtained in vitro that SAT does not produce this derivative.
Pegg
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AE
Department of Cell and Molecular Physiology, Milton S. Hershey Medical Center, Pennsylvania State University College of Medicine, Hershey 17033.
Pakala
R
R
Bergeron
R J
RJ
D000123:Acetyltransferases / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004790:Enzyme Induction; D015870:Gene Expression / Q000187:drug effects; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D011073:Polyamines / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D051381:Rats; D013096:Spermine / Q000031:analogs & derivatives* / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013997:Time Factors; D000099228:Diamine N-Acetyltransferase
D000123:Acetyltransferases; D003513:Cycloheximide; D003609:Dactinomycin; D011073:Polyamines; D012333:RNA, Messenger; D013096:Spermine; D000099228:Diamine N-Acetyltransferase; C059685:N(1),N(11)-diethylnorspermine