G3M T typing by dot immunobinding with monoclonal anti-G3M T antibody.
Dilutions of 100 serum samples of various GM phenotypes were dotted onto a nitrocellulose membrane. The serum dot-blots were detected with peroxidase-labeled anti-G3M T monoclonal antibody (anti-G3M T MAb). Up to a 1 : 256 dilution could be G3M T-typed correctly. By use of anti-G3M T MAb and peroxidase-labeled anti-mouse IgG or the biotin-avidin system instead of use of labeled anti-G3M T MAb, up to a 1 : 512 or 1 : 1024, respectively, dilution was typable. As with previous work with G3M G MAb, the dot immunobinding (DIB) method for G3M T typing was found very simple and practical.
Fukuda
M
M
Department of Forensic Medicine, Medical College of Oita, Japan.
Kishida
T
T
Tamaki
Y
Y
D000911:Antibodies, Monoclonal / Q000276:immunology*; D005819:Genetic Markers; D006801:Humans; D015151:Immunoblotting; D007129:Immunoglobulin Gm Allotypes / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D005819:Genetic Markers; D007129:Immunoglobulin Gm Allotypes