title: [Modification of H-phosphonate method of oligonucleotide synthesis on polymer support].
abstract: The mechanism of the internucleotide condensation and side-reactions in H-phosphonate approach has been investigated with the help of NMR-spectroscopy. On the basis of the results obtained a modification minimizing side-reactions in the course of the nucleotide component preactivation has been developed. It includes the use of acetonitrile--quinoline (4:1) mixture as the solvent in coupling reactions. The efficiency of the method is illustrated by the synthesis of oligodeoxynucleotides.
authors.forename: V A
authors.initials: VA
authors.lastname: Efimov
authors.forename: I Ia
authors.initials: IIa
authors.lastname: Dubeĭ
mesh_terms: D001483:Base Sequence; D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D008722:Methods; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000138:chemical synthesis*; D063065:Organophosphonates; D010766:Phosphorylation; D011804:Quinolines
chemical_list: D009841:Oligonucleotides; D063065:Organophosphonates; D011804:Quinolines; C037219:quinoline
vernacular_title: Modifikatsiia H-fosfonatnogo metoda sinteza oligonukleotidov na polimernykh nositeliakh.