Preparation of the nematode-trapping fungus, Arthrobotrys oligospora, for scanning electron microscopy by freeze substitution.
A freeze-substitution technique for preparing fungal specimens for scanning electron microscopy is described. This involves cryofixation in liquid nitrogen, freeze substitution in methanol at -20 degrees C and critical-point drying. The trapping complexes and conidiophores of the nematophagous fungus Arthrobotrys oligospora are well preserved and retain their normal three-dimensional arrangement.
Wharton
D A
DA
Department of Zoology, University of Otago, Dunedin, New Zealand.
Murray
D S
DS
D000818:Animals; D005615:Freezing; D008855:Microscopy, Electron, Scanning; D003904:Mitosporic Fungi / Q000502:physiology / Q000648:ultrastructure*; D012756:Sheep; D014254:Trichostrongylus / Q000502:physiology*