Chromatofocusing of aminoacyl-tRNA synthetases, extracted from NMRI mouse liver.
Chromatofocusing of 17 aminoacyl-tRNA synthetases extracted from NMRI mouse liver is described and the apparent isoelectric points of these enzymes are presented. Each of 15 aminoacyl-tRNA synthetases was present in two peaks. Isoleucyl-tRNA synthetase showed only one peak and arginyl-tRNA synthetase was present in three peaks. Phosphorylation/dephosphorylation experiments with arginyl-tRNA synthetase indicate that the peaks represent phosphorylated and unphosphorylated synthetase protein. One example of detection of increased protein phosphorylation during a biological experiment is presented.
Berg
B H
BH
Department of Biochemistry, University of Bergen, Norway.
D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification*; D000818:Animals; D002845:Chromatography / Q000379:methods; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000201:enzymology*; D051379:Mice; D008815:Mice, Inbred Strains; D010766:Phosphorylation
D000604:Amino Acyl-tRNA Synthetases