[Value of immunofixation for the typing of serum and urine monoclonal proteins].
Typing of monoclonal proteins can be performed, after screening by cellulose acetate electrophoresis, by immunoelectrophoresis (IEP) or immunofixation (IF). For sera, IF must be preferred to IEP because this methods is more sensitive and more easy to interpretate, particularly for small monoclonal components and biclonal abnormalities. For urine, patterns obtained by IF must be interpretated cautionaly because of the frequent polycondensed aspect of the non monoclonal light chains from tubular renal failures, and must be verified by a polyacrylamide agarose electrophoresis.
Boucaud-Maitre
Y
Y
Service de Biologie Médicale, Hôpital Saint-Joseph, Lyon.
Cloppet
H
H
Richard
M
M
D000911:Antibodies, Monoclonal / Q000302:isolation & purification* / Q000652:urine; D001788:Blood Grouping and Crossmatching; D003168:Complement Fixation Tests / Q000379:methods*; D004588:Electrophoresis, Cellulose Acetate / Q000379:methods; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods
D000911:Antibodies, Monoclonal
Valeur de l'immunofixation pour le typage des protéines monoclonales sériques et urinaires.