Assessment of an enzyme-linked immunosorbent assay using a Taenia hydatigena fraction antigen in the diagnosis of cysticercosis in cattle.
Enzyme linked immunosorbent assay (ELISA) using a fraction of larval Taenia hydatigena cyst fluid antigen was carried out on 469 bovine sera collected at slaughter from feedlot cattle for the presence of anticysticercosis antibodies. Cysticerci, in low numbers, were found in the heart, tongue and/or masseter muscles of 84 of the 469 cattle at postmortem inspection. Only nine sera gave positive ELISA reactions and in only one of these nine animals were cysticerci found. Within the limitations of this study, the high rate of false negative and false positive reactions suggests that the ELISA with the antigen used is not a satisfactory procedure to diagnose cysticercosis in cattle, at least in animals with light infections.
Smith
H J
HJ
Agriculture Canada, Health of Animals Laboratory, Sackville, New Brunswick.
Snowdon
K E
KE
Gregory
D
D
Finley
G G
GG
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis*; D000947:Antigens, Helminth / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D003551:Cysticercosis / Q000175:diagnosis / Q000662:veterinary*; D003552:Cysticercus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005188:False Negative Reactions; D005189:False Positive Reactions; D006321:Heart / Q000469:parasitology; D008406:Masseter Muscle / Q000469:parasitology; D011237:Predictive Value of Tests; D013621:Taenia / Q000276:immunology*; D014059:Tongue / Q000469:parasitology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth