Time-dependent inhibition of porcine kidney trehalase by aminosugars.
The inhibitory effects of various nitrogen-containing sugars on porcine kidney trehalase were studied. Validamycin A, validoxylamine A and MDL 25,637 were found to be potent, time-dependent inhibitors of the enzyme in vitro. The validoxylamine A-inhibited enzyme showed slow but reversible reactivation over time (t1/2 = 1.2 h). To our knowledge, this is the first report of time-dependent inhibition exhibited by either these particular aminosugars or a trehalase.
Salleh
H M
HM
Guelph-Waterloo Centre for Graduate Work in Chemistry, University of Waterloo, Ont. Canada.
Honek
J F
JF
D000606:Amino Sugars / Q000494:pharmacology*; D000818:Animals; D066298:In Vitro Techniques; D007294:Inositol / Q000031:analogs & derivatives / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects / Q000201:enzymology*; D007700:Kinetics; D013379:Substrate Specificity; D013402:Sugar Alcohols / Q000494:pharmacology; D013552:Swine; D014198:Trehalase / Q000037:antagonists & inhibitors*; D014199:Trehalose / Q000031:analogs & derivatives / Q000494:pharmacology
D000606:Amino Sugars; D013402:Sugar Alcohols; C052772:MDL 25637; C052534:validoxylamine A; D007294:Inositol; C003749:validamycins; D014199:Trehalose; D014198:Trehalase