A 13C NMR study of the application of [U-13C]succinate for metabolic investigations in rabbit renal proximal convoluted tubular cells.
The fate of the 13C label arising from [U-13C]succinate in rabbit renal proximal convoluted tubular (PCT) cells, incubated with [U-13C]succinate, was investigated by 13C NMR spectroscopy of the perchloric acid extracts of the cell pellets. The 13C label of [U-13C]succinate was predominantly metabolized into glutamate, glutamine, and into glucose, lactate, and alanine, which is the consequence of a carbon efflux of the label from the Krebs cycle at the stage of alpha-ketoglutarate and oxaloacetate, respectively.
Jans
A W
AW
Max-Planck-Institut für Systemphysiologie, Dortmund 1, Federal Republic of Germany.
Willem
R
R
D000818:Animals; D002247:Carbon Isotopes; D002478:Cells, Cultured; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D011817:Rabbits; D013386:Succinates / Q000378:metabolism*
D002247:Carbon Isotopes; D013386:Succinates