Phospholipid synthesis by extracellular phospholipase A2 in organic solvents.
The catalytic activity of extracellular phospholipase A2 was studied in low polarity solvents where hydrolytic enzymes have been demonstrated to catalyze synthesis reactions. It was demonstrated that extracellular phospholipase A2 can catalyze the esterification of lysophosphatidylcholine with oleic acid. Up to 6.5% of lysophosphatidylcholine can be esterified into phosphatidylcholine. This activity requires a preincubation of the enzyme in a pH 9 aqueous solution containing calcium, before the incubation in the non-aqueous solvent. No transfer of fatty acid between a phospholipid and a lysophospholipid or between two phospholipids was observed. These results may be useful in understanding the function of the membrane phospholipase A2 which may catalyze acylation or deacylation depending on the local physico-chemical environment.
Pernas
P
P
Laboratoire de Biochimie CHU St-Antoine, URA CNRS 217, Paris, France.
Olivier
J L
JL
Legoy
M D
MD
Bereziat
G
G
D002118:Calcium; D004951:Esterification; D008244:Lysophosphatidylcholines / Q000096:biosynthesis; D008246:Lysophospholipids / Q000096:biosynthesis; D010713:Phosphatidylcholines / Q000096:biosynthesis; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D010743:Phospholipids / Q000096:biosynthesis*; D012997:Solvents
D008244:Lysophosphatidylcholines; D008246:Lysophospholipids; D010713:Phosphatidylcholines; D010743:Phospholipids; D012997:Solvents; C008301:lysophosphatidylethanolamine; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D002118:Calcium