Characterization of the major sialidases of various types of rat blood cells: their comparison with rat liver sialidases.
The substrate specificity and subcellular location of the major sialidases of three types of rat blood cells were characterized and compared with those of the known three types of rat liver sialidase, which have been designated intralysosomal, cytosolic, and plasma membrane-associated sialidases. Platelets and leucocytes contain mainly an acid sialidase, which is highly active towards oligosaccharides and 4MU-NeuAc, and erythrocytes possess a high level of a sialidase acting on gangliosides. A Percoll gradient centrifugation study showed that the former is located in lysosomes and the latter in plasma membrane. When the sialidase was solubilized and partially purified from erythrocyte ghosts, the enzyme was found to hydrolyze actively gangliosides but only poorly other substrates such as 4MU-NeuAc, oligosaccharides, and glycoproteins. The sialidase partially purified from rat liver membrane fraction exhibited the same substrate specificity. It is concluded that the major sialidase of platelets and leucocytes corresponds to hepatic intralysosomal sialidase while erythrocytes contain almost exclusively a ganglioside sialidase which corresponds to hepatic plasma membrane sialidase.
Sagawa
J
J
Biochemistry Laboratory, Tohoku University, Miyagi.
Miyagi
T
T
Tsuiki
S
S
D000818:Animals; D001792:Blood Platelets / Q000201:enzymology; D002462:Cell Membrane / Q000201:enzymology; D002499:Centrifugation, Density Gradient; D004910:Erythrocyte Membrane / Q000201:enzymology; D066298:In Vitro Techniques; D007962:Leukocytes / Q000201:enzymology; D008099:Liver / Q000201:enzymology*; D008297:Male; D009439:Neuraminidase / Q000032:analysis / Q000097:blood*; D009844:Oligosaccharides / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000201:enzymology; D013379:Substrate Specificity
D009844:Oligosaccharides; D009439:Neuraminidase