title: Immunocytochemistry of cytoskeletal proteins in adult Schistosoma mansoni.
abstract: Antisera to vertebrate actin and actin-binding proteins were used to characterize the cytoskeleton of adult Schistosoma mansoni. Actin, alpha-actinin and tropomyosin immunoreactivities were detected in the cytoplasm of the apical tegument. Antiserum to alpha-actinin bound to the tegumental spines and this protein may be involved in cross-linking of spine actin filaments. Actin, alpha-actinin and tropomyosin antisera bound to the musculature. Strongest immunoreactivity was seen in the parenchyma. Antisera to actin, alpha-actinin, tropomyosin and spectrin bound to parenchyma cells including those of the tubercles, suggesting that these proteins are located in muscle cell bodies. The distribution of cytoskeletal proteins is discussed in relation to tegumental repair processes.
authors.affiliation: Department of Pure and Applied Biology, University of Leeds, U.K.
authors.forename: A N
authors.initials: AN
authors.lastname: MacGregor
authors.forename: S J
authors.initials: SJ
authors.lastname: Shore
mesh_terms: D000818:Animals; D003598:Cytoskeletal Proteins / Q000032:analysis*; D015801:Helminth Proteins / Q000032:analysis*; D007150:Immunohistochemistry; D008297:Male; D012550:Schistosoma mansoni / Q000032:analysis*
chemical_list: D003598:Cytoskeletal Proteins; D015801:Helminth Proteins