[Creation of an experimental cell culture vaccine against distemper using cell line 4647].
Experiment in susceptible animals demonstrated the effectiveness and safety of several samples of a distemper vaccine prepared in cell line 4647. Preparations of the vaccine virus variants generated in line 4647 after 10 passages at 34 degrees C, 37 degrees C and 40 degrees C and 20 passages of rapidly multiplying virus (34 degrees C) possessed the protective activity when used for immunization in a dose of 1000 PFU/animal. The results obtained in observations of the animals and examinations of sera for antibodies did not differ from those of control tests of "Vakchum".
Grachev
V P
VP
Kurbatov
A V
AV
Mironova
L L
LL
Popova
V D
VD
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004216:Distemper / Q000517:prevention & control*; D004217:Distemper Virus, Canine / Q000276:immunology* / Q000472:pathogenicity; D012889:Mephitidae; D014611:Vaccination / Q000662:veterinary*; D014765:Viral Vaccines / Q000096:biosynthesis*; D014774:Virulence
D014765:Viral Vaccines
Sozdanie éksperimental'noĭ kul'tural'noĭ vaktsiny protiv chumy plotoiadnykh s ispol'zovaniem kletok 4647.