[Determination of cerebrospinal fluid proteins using the Cobas Fara centrifugal analyser. Adaptation of the turbidimetric technic to benzethonium chloride].
The authors have adapted a turbidimetric assay on centrifugal Cobas Fara analyser for cerebrospinal fluid proteins determinations. The later method is proposed by SFBC Protein Analysis Committee using benzethonium chloride as monoreagent. This adaptation is more sensitive than manual method. The linearity range is greater (0.08-1.85 g/l instead of 0.2-1.2 g/l). Sample assay and time of analysis were reduced by 90 p. cent.
Guérin
J L
JL
Département de Biologie Clinique, Centre Claudius-Régaud, Toulouse.
Favre
G
G
Raynal
G
G
Soula
G
G
D001558:Benzethonium; D002498:Centrifugation / Q000295:instrumentation*; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D006801:Humans; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation* / Q000379:methods; D000644:Quaternary Ammonium Compounds
D002556:Cerebrospinal Fluid Proteins; D000644:Quaternary Ammonium Compounds; D001558:Benzethonium
Détermination de la protéinorachie sur analyseur centrifuge Cobas Fara. Adaptation de la technique turbidimétrique au chlorure de benzethonium.