Complement activation before, during and after cardiopulmonary bypass.
Plasma levels of the complement parent molecules C3, C4, and factor B and their split products, C3d, C4d, and Ba were measured in 12 patients undergoing cardiopulmonary bypass for coronary artery surgery. Alternative and common complement pathway activation, demonstrated by statistically significant rising levels of Ba (P less than 0.05), and C3d (P less than 0.05) and by elevated Ba:B (P less than 0.05) and C3d:C3 (P less than 0.05) ratios were found before the institution of cardiopulmonary bypass but following heparin administration suggesting that heparin may itself initiate alternative pathway activation. In addition, significant depletion of parent complement components and elevation of split product concentrations was seen during bypass suggesting classical and alternate pathway activation (P less than 0.01). This study clarifies the pathways of complement activation during bypass and presents evidence that heparin administration may initially activate the complement cascade.
Bonser
R S
RS
Department of Surgery, Brompton Hospital, London, UK.
Dave
J R
JR
John
L
L
Gademsetty
M K
MK
Carter
P G
PG
Davies
E
E
Taylor
P
P
Gaya
H
H
Lennox
S C
SC
Vergani
D
D
D002315:Cardiopulmonary Bypass; D003167:Complement Activation / Q000187:drug effects / Q000276:immunology*; D003176:Complement C3 / Q000032:analysis; D015933:Complement C3d / Q000032:analysis; D003181:Complement C4 / Q000032:analysis; D015935:Complement C4b; D011415:Complement Factor B / Q000032:analysis; D001026:Coronary Artery Bypass; D006493:Heparin / Q000627:therapeutic use; D006801:Humans; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000032:analysis
D003176:Complement C3; D003181:Complement C4; D010446:Peptide Fragments; D015933:Complement C3d; D015935:Complement C4b; C032261:complement C4d; D006493:Heparin; D011415:Complement Factor B