title: Purification and partial characterization of CD9 antigen of human platelets.
abstract: CD9 antigen (p24) was purified from human platelets and partially characterized. The yield was 75 micrograms from 10 units of platelet concentrates. p24 (38,000 copies/platelet) has intramolecular disulfide bond(s) and, in SDS-PAGE, consists of major 24-kDa molecule and minor 26- to 31-kDa molecules. The N-terminal sequence of p24, PVKGGTKXIKYLLFGFNFIF, indicates that the protein has not previously been characterized and amino terminus (position 12-20) is hydrophobic.
authors.affiliation: First Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Higashihara
authors.forename: K
authors.initials: K
authors.lastname: Takahata
authors.forename: Y
authors.initials: Y
authors.lastname: Yatomi
authors.forename: K
authors.initials: K
authors.lastname: Nakahara
authors.forename: K
authors.initials: K
authors.lastname: Kurokawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D015703:Antigens, CD / Q000302:isolation & purification*; D000943:Antigens, Differentiation / Q000302:isolation & purification*; D001792:Blood Platelets / Q000276:immunology*; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D060245:Tetraspanin 29
chemical_list: D000906:Antibodies; D015703:Antigens, CD; D000943:Antigens, Differentiation; C554869:CD9 protein, human; D008562:Membrane Glycoproteins; D060245:Tetraspanin 29