(3H/14C)beta-methylheptadecanoic acid subcellular distribution and lipid incorporation in mouse heart.
Beta-methylfatty acids are transported into myocardial cells as other fatty acids would be, but are incapable, for example, of undergoing complete catabolism. Our previous studies have established the structure-location relationship and the imaging characteristics of these analogs. In the present study in mouse myocardium, microautoradiography and electron microscopy were used to show the distribution of [7,8-3H] beta-methylheptadecanoic acid [( 3H]BMHA) in mitochondria and lipid droplets. Thin-layer chromatography demonstrated the presence of [1-14C] beta-methylheptadecanoic acid [( 14C]BMHA) and its metabolites in various lipid pools. These studies complement our earlier findings which showed that similarities exist in the initial metabolic fate of BMHA and physiological fatty acids.
Livni
E
E
Massachusetts General Hospital, Department of Radiology, Boston 02114.
Ito
S
S
Kassis
A I
AI
Elmaleh
D R
DR
D000818:Animals; D001345:Autoradiography; D005227:Fatty Acids / Q000378:metabolism*; D050356:Lipid Metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D008929:Mitochondria, Heart / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism* / Q000648:ultrastructure; D013347:Subcellular Fractions / Q000378:metabolism
D005227:Fatty Acids; C033637:3-methylheptadecanoic acid