Inactivation of human synovial fluid phospholipase A2 by the marine natural product, manoalide.
The marine natural product, manoalide (MLD), was investigated to determine if this drug inhibited purified human synovial fluid phospholipase A2 (HSF-PLA2). Utilizing classical Michaelis-Menten kinetics, apparent Km and Vmax values for HSF-PLA2 of 1.34 mM and 0.47 mumol [3H]palmitic acid released/min/mg protein were obtained using dipalmitoylphosphatidylcholine (DPPC) as the substrate, and 38.0 microM and 18.8 mumol [3H]arachidonic acid released/min/mg protein with Escherichia coli as a natural substrate. These kinetic parameters were utilized subsequently to evaluate the inhibitory effects of manoalide on HSF-PLA2. Inhibition of HSF-PLA2 by MLD was concentration and time dependent with IC50 values of 0.2 and 0.02 microM for DPPC and E. coli respectively. Dialysis studies and examination of DPPC or E. coli hydrolysis versus enzyme concentration indicate that MLD is an irreversible inhibitor of HSF-PLA2. Substrate specificity was also examined in the absence and presence of MLD using dipalmitoylphosphatidylethanolamine (DPPE) as a substrate. MLD inhibited the hydrolysis of DPPE (greater than 90% inhibition at 2 microM), and preliminary results indicate that DPPC was more readily hydrolyzed than DPPE under the substrate conditions of the assay. While the cellular source of secreted HSF-PLA2 is unknown, these studies indicate that MLD can inactivate secreted phospholipase A2 isolated from patients with inflammatory joint disease.
Jacobson
P B
PB
Department of Biological Sciences, University of California, Santa Barbara 93106.
Marshall
L A
LA
Sung
A
A
Jacobs
R S
RS
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000378:metabolism; D003956:Dialysis; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D006801:Humans; D007592:Joint Diseases / Q000201:enzymology; D007700:Kinetics; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010740:Phospholipases / Q000037:antagonists & inhibitors*; D010741:Phospholipases A / Q000037:antagonists & inhibitors*; D054467:Phospholipases A2; D013582:Synovial Fluid / Q000201:enzymology*; D013729:Terpenes / Q000494:pharmacology*
D005230:Fatty Acids, Nonesterified; D010714:Phosphatidylethanolamines; D013729:Terpenes; D015060:1,2-Dipalmitoylphosphatidylcholine; C007510:1,2-dipalmitoyl-3-phosphatidylethanolamine; C045673:manoalide; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2