Glycine antagonists block the induction of long-term potentiation in CA1 of rat hippocampal slices.
The effects of the strychnine-insensitive glycine receptor antagonists, cycloleucine and 7-chlorokynurenic acid, on the induction of long-term potentiation (LTP) in CA1 of rat hippocampal slices were examined. A 5 min administration of cycloleucine (20-100 microM) or 7-chlorokynurenic acid (1-5 microM) during the delivery of high-frequency stimulation blocked the induction of LTP without affecting baseline synaptic transmission. Coapplication of 100 microM glycine with cycloleucine or 7-chlorokynurenic acid masked the inhibitory effect on the induction of LTP, supporting the hypothesis that these compounds act as glycine antagonists. These results indicate that glycine is a necessary factor for the induction of LTP in CA1 of the rat hippocampus.
Izumi
Y
Y
Department of Psychiatry, Washington University School of Medicine, St. Louis, MO 63110.
Clifford
D B
DB
Zorumski
C F
CF
D000200:Action Potentials / Q000187:drug effects; D000222:Adaptation, Physiological / Q000187:drug effects*; D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D003515:Cycloleucine / Q000494:pharmacology*; D005998:Glycine / Q000037:antagonists & inhibitors / Q000502:physiology*; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D007736:Kynurenic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats
D000596:Amino Acids; D003515:Cycloleucine; D007736:Kynurenic Acid; C057013:7-chlorokynurenic acid; D005998:Glycine