Characterization of a cDNA encoding rat sterol carrier protein2.
Sterol carrier protein2 (SCP2) is a 13.2-kD protein that is thought to be involved in the intracellular transport of cholesterol. Using synthetic oligonucleotides based on the protein sequence of SCP2, a clone (SP43) was isolated from a rat liver cDNA library. The DNA sequence revealed that the cDNA could encode a polypeptide of 273 amino acids (28.9 kD) or 143 amino acids (15.3 kD) in which the carboxy-terminal 123 amino acids are identical to the SCP2 protein. RNA blot hybridization revealed that a variety of rat tissues contain a homologous RNA of a size similar to SP43 (approximately 1.5 kb). Levels of SCP2 mRNA increased in parallel with cytochrome P450scc mRNA in the immature gonadotropin-primed rat ovary. The isolation of a cDNA clone encoding SCP2 will facilitate studies on its role in cholesterol metabolism.
Billheimer
J T
JT
Medical Products Department, E.I. DuPont de Nemours & Co. Inc., Wilmington, DE 19880.
Strehl
L L
LL
Davis
G L
GL
Strauss
J F
JF
Davis
L G
LG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002352:Carrier Proteins / Q000235:genetics*; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D015342:DNA Probes; D005260:Female; D006064:Gonadotropins, Equine / Q000494:pharmacology; D008099:Liver / Q000032:analysis; D008969:Molecular Sequence Data; D010053:Ovary / Q000378:metabolism; D010940:Plant Proteins; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D013261:Sterols / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects
D002352:Carrier Proteins; D006063:Chorionic Gonadotropin; D015342:DNA Probes; D006064:Gonadotropins, Equine; D010940:Plant Proteins; D012333:RNA, Messenger; D013261:Sterols; C072087:sterol carrier proteins; D004247:DNA