title: IPGMAKER: a program for IBM-compatible personal computers to create and test recipes for immobilized pH gradients.
abstract: The program "IPGMAKER" is a computational aid for creating and testing recipes for near-linear immobilized pH gradients. It was written for fast IBM personal computers (with a Type 80386 processor and 80387 coprocessor) and compatibles equipped with a VGA, EGA or Hercules (mono) graphics card. The program is limited to the use of up to 10 acids and/or bases, and to ranges spanning between pH 2 and 12. The resulting recipes are presented either as final concentrations in the 2 chambers of a mixing device for linear gradients or as volumes from 0.2 moles/L stock solutions adjusted to a user-defined average buffering power. One of the subroutines determines the pH, gradient slope and buffering capacity at any location of the gradient and includes a facility to estimate the pI of proteins from the composition of their primary structure.
authors.affiliation: University of Giessen, Federal Republic of Germany.
authors.forename: K
authors.initials: K
authors.lastname: Altland
mesh_terms: D000178:Acrylamides; D002021:Buffers; D003201:Computers; D004586:Electrophoresis / Q000379:methods*; D005782:Gels; D006863:Hydrogen-Ion Concentration; D012984:Software
chemical_list: D000178:Acrylamides; D002021:Buffers; D005782:Gels; C059413:Immobiline