title: Herpes simplex virus type I infection of the CNS induces major histocompatibility complex antigen expression on rat microglia.
abstract: Rats were infected with herpes simplex virus type I (HSV-1) by corneal scarification. The spread of virus in the brain, the infiltration of leucocytes into infected areas, and the expression of major histocompatibility complex (MHC) glycoproteins by brain cells were assessed as a function of time by immunohistochemistry. Virus moved along neuronal pathways, achieving widespread distribution in the brain by days 8-10 when the illness appeared most severe. Granulocytes, T-lymphocytes, and monocytes infiltrated the tissue matrix at sites of infection. Microglial cells were induced to express MHC class I and class II glycoproteins. Reactive microglia near the sites of infection most vigorously expressed such glycoproteins. At the peak of the infection they were detectable on microglia throughout the brain, including areas apparently separated from active infection. Evidence of viral antigens, as well as microglial MHC expression, had largely disappeared by day 30. Neurons, astrocytes, and oligodendroglial cells failed to express MHC antigens.
authors.affiliation: Kinsmen Laboratory of Neurological Research, Department of Psychiatry, University of British Columbia, Vancouver, Canada.
authors.forename: D L
authors.initials: DL
authors.lastname: Weinstein
authors.forename: D G
authors.initials: DG
authors.lastname: Walker
authors.forename: H
authors.initials: H
authors.lastname: Akiyama
authors.forename: P L
authors.initials: PL
authors.lastname: McGeer
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000378:metabolism*; D001927:Brain Diseases / Q000276:immunology* / Q000473:pathology; D006561:Herpes Simplex / Q000276:immunology* / Q000503:physiopathology; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D008297:Male; D009457:Neuroglia / Q000276:immunology* / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000914:Antibodies, Viral; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II