title: Bromodeoxyuridine incorporation into DNA of human leukemia cells is not concentration dependent.
abstract: Leukemia blasts isolated from bone marrow aspirates of 44 adults with acute leukemia were incubated for 1 h with 0.008-32 microM bromodeoxyuridine (Brd-Urd). After dual labeling with monoclonal anti-BrdUrd antibodies and propidium iodide, the cells were analyzed by flow cytometry. Percent labeled cells and intensity of labeling were similar over concentrations of BrdUrd ranging from 0.8-32 microM--a 40-fold range. Therefore, despite potential interpatient variability in nucleoside pharmacokinetics, commonly used doses of BrdUrd which are intended to achieve steady-state plasma concentrations in the 8.0 microM range can be expected to provide a reliable estimate of the S-phase fraction.
authors.affiliation: Cancer Center, Wake Forest University, Winston-Salem, North Carolina 27103.
authors.forename: B L
authors.initials: BL
authors.lastname: Powell
authors.forename: B W
authors.initials: BW
authors.lastname: Gregory
authors.forename: T E
authors.initials: TE
authors.lastname: Kute
authors.forename: T M
authors.initials: TM
authors.lastname: Morgan
authors.forename: E S
authors.initials: ES
authors.lastname: Lyerly
authors.forename: R L
authors.initials: RL
authors.lastname: Capizzi
mesh_terms: D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine / Q000493:pharmacokinetics*; D004273:DNA, Neoplasm / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000378:metabolism; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D004273:DNA, Neoplasm; D001973:Bromodeoxyuridine