Oligosaccharide Y specific monoclonal antibody and its isotype switch variants.
Isotype switch variants of the IgG3 secreting hybridoma BR55-2 were developed with gamma 1, gamma 2b and gamma 2a heavy chains. The variants have the same affinity for antigen and expected affinities for the Fc receptor. The Y antigen defined by MAb BR55-2 has a restricted distribution to breast, colorectal, pancreatic, gastric and small cell lung carcinomas. The MAb BR55-2 also bound to prostatic and laryngeal carcinomas. The BR55-2 defined epitope is expressed by gastrointestinal cancer cell lines as a glycolipid, while the breast carcinoma cells express the same epitope on glycoproteins. These differences may influence tumor cell susceptibility to lysis by MAb BR55-2 and its isotype switch variants.
Steplewski
Z
Z
Wistar Institute of Anatomy and Biology, Philadelphia, PA.
Blaszczyk-Thurin
M
M
Lubeck
M
M
Loibner
H
H
Scholz
D
D
Koprowski
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity; D000918:Antibody Specificity; D000941:Antigens / Q000032:analysis; D001943:Breast Neoplasms; D002240:Carbohydrate Sequence; D002460:Cell Line; D002478:Cells, Cultured; D014644:Genetic Variation; D006017:Glycolipids / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology; D006028:Glycosphingolipids / Q000032:analysis*; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007132:Immunoglobulin Isotypes / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D014407:Tumor Cells, Cultured / Q000032:analysis
D000911:Antibodies, Monoclonal; D000941:Antigens; D006017:Glycolipids; D006023:Glycoproteins; D006028:Glycosphingolipids; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; C055089:Y glycolipid