Molecular cloning of a cDNA encoding the bombesin precursor in skin of Bombina variegata.
A cDNA library was constructed using poly(A)-rich RNA isolated from skin of the frog Bombina variegata. This library was screened with two oligo-nucleotides complementary to parts of the sequence of bombesin. The nucleotide sequence of one of the cloned cDNAs encoding a bombesin precursor is presented. The predicted polypeptide contains a single copy of the end-product. The bombesin sequence is preceded by a leucine residue suggesting an unusual type of precursor processing.
Richter
K
K
Institute of Molecular Biology, Austrian Academy of Sciences, Salzburg.
Egger
R
R
Kreil
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001001:Anura / Q000235:genetics*; D001483:Base Sequence; D001839:Bombesin / Q000096:biosynthesis / Q000235:genetics*; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000096:biosynthesis / Q000235:genetics; D012867:Skin / Q000378:metabolism*
D011498:Protein Precursors; D001839:Bombesin