The modulation of gap junctional communication by gossypol in various mammalian cell lines in vitro.
The effects of gossypol were examined in several cell lines including hamster V79 lung fibroblasts, WB-344 rat liver oval cells, human osteosarcoma cells and LC540 rat Leydig cells. Gossypol had little cytotoxic effects in these cell lines except at high concentrations. Plasma membrane integrity was maintained in LC540 except at high concentrations of gossypol. Gossypol did not increase mutational frequency as examined by the hypoxanthine guanine phosphoribosyl transferase and Na+,K(+)-ATPase loci in V79 cells. Gossypol inhibited gap junctional intercellular communication in some but not all of the cell lines. This selectivity might be the basis for the sensitivity of certain tissues or organs to gossypol. For example, the Leydig cell, which is the component of a target organ system for toxicity, was sensitive to gossypol. Modulation of gap junctional functions might play a significant role in both pharmacological and toxicological effects of gossypol.
Ye
Y X
YX
Department of Pharmacology and Toxicology, Michigan State University, East Lansing 48824.
Bombick
D
D
Hirst
K
K
Zhang
G X
GX
Chang
C C
CC
Trosko
J E
JE
Akera
T
T
D000818:Animals; D002450:Cell Communication / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006072:Gossypol / Q000494:pharmacology* / Q000633:toxicity; D006801:Humans; D007365:Intercellular Junctions / Q000187:drug effects*; D009152:Mutagenicity Tests; D051381:Rats; D013997:Time Factors
D006072:Gossypol