Electrophysiological characteristics of cholinergic and non-cholinergic neurons in the rat medial septum-diagonal band complex.
We examined the electrophysiological properties of cholinergic and non-cholinergic neurons in the medial septum-diagonal band complex (MSDB) of the rat in the in vitro slice preparation. Cells were identified electrophysiologically, filled with Lucifer yellow, fixed and processed for immunohistochemistry with fluorescent labeled anti-choline acetyltransferase (ChAT) antibody. Cholinergic and non-cholinergic neurons differed in action potential parameters, spike afterpotentials and in current-voltage relationships. In addition, cholinergic neurons expressed a potent transient outward rectification in response to a depolarizing current pulse.
Markram
H
H
Center for Neuroscience, Weizmann Institute, Rehovot, Israel.
Segal
M
M
D000200:Action Potentials; D000818:Animals; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D002799:Cholinergic Fibers / Q000201:enzymology / Q000502:physiology*; D005625:Frontal Lobe / Q000502:physiology*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D051381:Rats; D012686:Septal Nuclei / Q000502:physiology*
D002795:Choline O-Acetyltransferase