Platelet functions in recombinant hirudin-anticoagulated blood.
The influence of genetically engineered recombinant hirudin (r-hirudin) on platelet functions was studied. Depending on the concentration, r-hirudin inhibits the thrombin-induced aggregation and 14C-serotonin secretion to the same extent as native hirudin. Comparative studies in blood anticoagulated by r-hirudin, heparin or citrate show a significantly lower spontaneous platelet aggregation in r-hirudinized blood. The extent of the ADP-induced aggregation is nearly the same in r-hirudinized, heparinized or citrated plasma. In r-hirudinized plasma, however, aggregation is reversible. In contrast to heparinized or citrated plasma, adrenaline causes only a very slight aggregation in r-hirudinized plasma. ADP- or adrenaline-induced secretion of 14C-serotonin do not occur in r-hirudinized plasma. The collagen- as well as the PAF-induced aggregation and 14C-serotonin release in hirudinized plasma do not differ significantly from those in heparinized or citrated plasma. r-Hirudin is a suitable anticoagulant for studying platelet functions because it does not produce any alterations in platelet reactions and does not provoke any changes in the ionized calcium concentration in blood.
Glusa
E
E
Institute of Pharmacology and Toxicology, Medical Academy, Erfurt, GDR.
Markwardt
F
F
D000925:Anticoagulants / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects*; D002951:Citrates / Q000494:pharmacology; D019343:Citric Acid; D006493:Heparin / Q000494:pharmacology; D006629:Hirudins / Q000494:pharmacology*; D006801:Humans; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D012701:Serotonin / Q000378:metabolism; D013917:Thrombin / Q000502:physiology
D000925:Anticoagulants; D002951:Citrates; D006629:Hirudins; D010975:Platelet Aggregation Inhibitors; D011994:Recombinant Proteins; D019343:Citric Acid; D012701:Serotonin; D006493:Heparin; D013917:Thrombin