Reversal of creatine kinase translational repression by 3' untranslated sequences.
A subline of U937 cells (U937D) was obtained in which creatine kinase B (CK-B) messenger RNA was present and bound to ribosomes, but CK activity was undetectable. Transformation of U937D cells with retrovirus vectors that contain the 3' untranslated region (3' UTR) of CK-B messenger RNA exhibited CK activity with no change in abundance of CK-B mRNA. The 3' UTR formed a complex in vitro with a component of S100 extracts from wild-type cells. This binding activity was not detectable in S100 extracts from cells that expressed CK activity after transformation with the 3' UTR-containing vector. These results suggest that translation of CK-B is repressed by binding of a soluble factor or factors to the 3' UTR.
Ch'ng
J L
JL
Department of Medicine, Duke University, Durham, NC 27710.
Shoemaker
D L
DL
Schimmel
P
P
Holmes
E W
EW
D002460:Cell Line; D003001:Cloning, Molecular; D003402:Creatine Kinase / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*
D012333:RNA, Messenger; D007041:Hypoxanthine Phosphoribosyltransferase; D003402:Creatine Kinase