title: [Presence of flagellar antigenic subfactors in serotype 1 of Bacillus thuringiensis].
abstract: The strain G was isolated from a dead army worm (Leucania separata Walker) in corn field, at Hebei. It has the typical morphological characteristics of Bacillus thuringiensis. The serological analysis showed that the flagellar suspensions of the strain G was agglutinated only by the antisera against the H1 antigen and not by the antisera against the other 22 known H-antigens. The agglutination titre was 1:6,400. The flagellar suspension of the serotype 1, subsp. thuringiensis, was agglutinated by the antiserum against the strain G, with a titre of 1:12,800. However, the cross-saturation of antisera showed that there were different fractions in the H1 antigen. The H antiserum against subsp. thuringiensis still agglutinated with subsp. thuringiensis, having a titre of 1:6,400, even if it was fully saturated by strain G. When it was saturated by subsp. thuringiensis the H antiserum of strain G did not agglutinate by himself. Therefore, the H1 antigen (subsp. thuringiensis) must be divided into subfactors H1alb and strain G antigen is H1a. On the basis of this study, we propose the strain G represents the serotype H1a under the name of Bacillus thuringiensis subsp. hebeiensis.
authors.affiliation: Institute of Zoology, Academia Sinica, Beijing.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Wen
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Wang
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001413:Bacillus thuringiensis / Q000145:classification* / Q000302:isolation & purification; D007915:Lepidoptera / Q000382:microbiology*; D012703:Serotyping
chemical_list: D000942:Antigens, Bacterial; C022863:H antigen