Aqueous flare measurement with laser flare-cell meter.
The laser flare-cell meter is a newly developed instrument designed to measure anterior chamber protein concentration in vivo. There was a linear relationship between photon count measured with this instrument and the concentration of bovine serum albumin. Measurements were taken in 29 eyes of normal subjects divided into two groups. Group 1 (15 eyes) had brown or dark irises and Group 2 (14 eyes) had blue or green irises. Photon counts obtained from Groups 1 and 2 were not significantly different. Aqueous protein concentrations estimated from standard curves were 15.7 and 18.6 mg/100 ml for Groups 1 and 2, respectively. On the other hand, the background value obtained from Group 2 was about twice the value from Group 1. These results indicate that background scattering has little effect on photon count. There was a good correlation between photon counts measured with the laser flare-cell meter and the evaluation of flare made by conventional microscopic observations. It was concluded that this instrument will be useful for clinical research studies.
Yoshitomi
T
T
Department of Ophthalmology and Visual Science, Yale University School of Medicine, New Haven, CT 06510.
Wong
A S
AS
Daher
E
E
Sears
M L
ML
D000328:Adult; D000867:Anterior Chamber / Q000378:metabolism*; D001082:Aqueous Humor / Q000378:metabolism*; D002452:Cell Count; D005069:Evaluation Studies as Topic; D005127:Eye Color / Q000502:physiology; D005136:Eye Proteins / Q000378:metabolism*; D006801:Humans; D007834:Lasers; D008875:Middle Aged; D012542:Scattering, Radiation; D012710:Serum Albumin, Bovine / Q000378:metabolism
D005136:Eye Proteins; D012710:Serum Albumin, Bovine