Cultured vascular smooth muscle cells from porcine coronary artery possess A1 and A2 adenosine receptor activity.
This study tested the hypothesis that an A1 adenosine receptor capable of inhibiting adenylate cyclase activity is present in porcine coronary vascular smooth muscle cells. In the absence of blockade of the A2 adenosine receptor, the A1 adenosine receptor agonists phenylisopropyladenosine (PIA) and cyclopentyladenosine (CPA) (10(-9) M) failed to inhibit Gpp(NH)p stimulated adenylate cyclase activity. However, after blockade of the A2 adenosine receptor with 30 nM CGS 15943A, cyclopentyladenosine (10(-9) M) inhibited Gpp(NH)p stimulated adenylate cyclase activity by 27 +/- 3% (4.3 +/- 0.7, Mean +/- SEM; pmoles/min/mg vs 5.9 +/- 0.8, P less than .05). The data demonstrate that both A1 and A2 adenosine receptors are present in coronary vascular smooth muscle. The results indicate that adenosine may mediate both vasodilation and vasoconstriction in the coronary circulation via A2 and A1 adenosine receptors, respectively.
Mills
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Division of Cardiology, Rhode Island Hospital, Providence 02903.
Gewirtz
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H
D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D000067956:Adenylyl Cyclase Inhibitors; D000704:Analysis of Variance; D000818:Animals; D002478:Cells, Cultured; D003331:Coronary Vessels; D009131:Muscle, Smooth, Vascular / Q000201:enzymology / Q000378:metabolism*; D010660:Phenylisopropyladenosine / Q000494:pharmacology; D011799:Quinazolines / Q000494:pharmacology; D011983:Receptors, Purinergic / Q000378:metabolism*; D013552:Swine; D014230:Triazoles / Q000494:pharmacology
D000067956:Adenylyl Cyclase Inhibitors; D011799:Quinazolines; D011983:Receptors, Purinergic; D014230:Triazoles; D010660:Phenylisopropyladenosine; C048599:N(6)-cyclopentyladenosine; D000241:Adenosine; C052410:9-chloro-2-(2-furyl)-(1,2,4)triazolo(1,5-c)quinazolin-5-imine