Cloning and expression of a human voltage-gated potassium channel. A novel member of the RCK potassium channel family.
We have isolated and characterized a human cDNA (HBK2) that is homologous to novel member (RCK2) of the K+ channel RCK gene family expressed in rat brain. RCK2 mRNA was detected predominantly in midbrain areas and brainstem. The primary sequences of the HBK2/RCK2 K+ channel proteins exhibit major differences to other members of the RCK gene family. The bend region between segments S1 and S2 is unusually long and does not contain the N-glycosylation site commonly found in this region. They might be O-glycosylated instead. Functional characterization of the HBK2/RCK2 K+ channels in Xenopus laevis oocytes following micro-injection in in vitro transcribed HBK2 or RCK2 cRNA showed that the HBK2/RCK2 proteins form voltage-gated K+ channels with novel functional and pharmacological properties. These channels are different to RCK1, RCK3, RCK4 and RCK5 K+ channels.
Grupe
A
A
Ruhr-Universität Bochum, Lehrstuhl für Biochemie, FRG.
Schröter
K H
KH
Ruppersberg
J P
JP
Stocker
M
M
Drewes
T
T
Beckh
S
S
Pongs
O
O
D000818:Animals; D001483:Base Sequence; D001693:Biological Transport, Active; D001921:Brain / Q000378:metabolism*; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D015870:Gene Expression; D006801:Humans; D066298:In Vitro Techniques; D051667:Kv1.6 Potassium Channel; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D015183:Restriction Mapping; D051637:Shaker Superfamily of Potassium Channels; D014982:Xenopus laevis / Q000235:genetics*
C064389:KCNA6 protein, human; C064390:Kcna6 protein, rat; D051667:Kv1.6 Potassium Channel; D008565:Membrane Proteins; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D012333:RNA, Messenger; D051637:Shaker Superfamily of Potassium Channels; D004247:DNA