Differential limb regeneration in diploid and triploid Rana pipiens larvae with reference to spinal motor neuron development.
Developmental manipulations that can alter nerve-limb relationships can assist in understanding the neural control of limb regeneration. Pressure-induced triploidy in Rana pipiens tadpoles results in alterations of the quantitative characteristics of the spinal motor neurons that innervate the limbs, whereas the limbs appear unaltered. Unilateral midthigh amputations at larval stages IX, XI, and XIII of diploid and triploid animals resulted in complete regeneration for only stage IX animals regardless of ploidy. Nevertheless, triploid limbs regenerated much faster than did diploids, an event that can be related to the differential dynamics of nerve fiber extension and/or the altered numbers and sizes of triploid spinal motor neurons. Although normal limb development from stage IX to the endpoint at stage XVIII was the same in diploids and triploids, the rate of regeneration in triploids was nearly twice that of diploids. The data of this noninvasive means of altering the quantitative relationship of nerve-to-peripheral target suggest a unique means of studying nerve-dependent limb regeneration in an animal that progressively loses its regenerative capability during development.
Pollack
E D
ED
Institute for the Study of Developmental Disabilities, University of Illinois, Illinois 60680.
Maheras-Rarick
H M
HM
D000818:Animals; D002452:Cell Count; D004171:Diploidy; D006614:Hindlimb; D007814:Larva; D009046:Motor Neurons / Q000502:physiology*; D011003:Ploidies; D011312:Pressure; D011894:Rana pipiens; D012038:Regeneration / Q000235:genetics* / Q000502:physiology; D013116:Spinal Cord / Q000166:cytology / Q000196:embryology* / Q000502:physiology