A bovine homolog to the human myogenic determination factor myf-5: sequence conservation and 3' processing of transcripts.
A bovine cDNA library from fetal skeletal muscle myoblasts. was screened with a 274 bp probe to a conserved region of the mouse MyoD1 cDNA. One positive recombinant, designated bmyf, was found to contain a 1931 bp insert with an open reading frame encoding a predicted protein highly related to the human myogenic factor myf-5 Human and bovine factors are 96% homologous in their predicted amino acid sequences. At the nucleotide level, bmyf and myf-5 are 92% identical in the coding region and 74 and 80% homologous in their 5'- and 3'-untranslated regions, respectively. The bmyf cDNA, nevertheless, extends 475 nucleotides beyond a polyadenylation signal common to both cDNAs. Bmyf transcripts are expressed exclusively in skeletal muscle where three transcripts of 1.5, 2 and 3 kb were detected. While the 1.5 kb transcript lacks sequences 3' to the polyadenylation signal at nt 1415 in the bmyf cDNA, both the 2 and 3 kb RNAs contain these sequences suggesting that bmyf transcripts are alternatively polyadenylated. Bmyf cDNA can activate the expression of the myogenic program in C3H10T1/2 fibroblasts as assayed by stable and transient transfection experiments.
Clark
T G
TG
Department of Genetics, University of Georgia, Athens 30602.
Morris
J
J
Akamatsu
M
M
McGraw
R
R
Ivarie
R
R
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002417:Cattle; D002460:Cell Line; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004268:DNA-Binding Proteins; D006801:Humans; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics*; D051787:Myogenic Regulatory Factor 5; D012323:RNA Processing, Post-Transcriptional; D012689:Sequence Homology, Nucleic Acid; D015534:Trans-Activators; D014158:Transcription, Genetic; D014162:Transfection
D004268:DNA-Binding Proteins; C497417:MYF5 protein, human; D009124:Muscle Proteins; D051787:Myogenic Regulatory Factor 5; D015534:Trans-Activators; D004247:DNA