R-banding and in situ hybridization localization of single copy genes on high resolution banded chromosomes.
A method to preserve high resolution R-bands on human lymphocyte chromosomes after in situ hybridization to 3H-labelled probes and autoradiography is presented. The technique was used to re-examine the location of the known oncogene N-myc. Due to the high resolution power of the present technique, this gene could be located to subband 2p24.1 in the human karyotype.
Rønne
M
M
Institute of Anatomy and Cytology, Odense University, Denmark.
Kirpekar
F
F
Shibasaki
Y
Y
Poulsen
B S
BS
Kristiansen
K
K
D002871:Chromosome Banding / Q000379:methods*; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D006801:Humans; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D014960:X Chromosome