Site-directed mutagenesis of ricin A chain Trp 211 to Phe.
Oligonucleotide-directed site-specific mutagenesis has been used to make a conservative change in the putative active site of the catalytic subunit of the toxin ricin. The substitution of phenylalanine for tryptophan at position 211 of the A chain reduces but does not abolish catalytic activity, as assayed by inhibition of cell-free protein synthesis.
Bradley
J L
JL
Department of Biochemistry and Molecular Biology, University of Florida, Gainesville.
McGuire
P M
PM
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D008969:Molecular Sequence Data; D009154:Mutation; D010649:Phenylalanine; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012276:Ricin / Q000235:genetics* / Q000378:metabolism; D014364:Tryptophan
D011994:Recombinant Proteins; D010649:Phenylalanine; D014364:Tryptophan; D012276:Ricin