Conjugation of a monoclonal antibody with a DTPA modified random copolymer of hydroxyethyl methylacrylate and methyl methacrylate.
A new approach for covalent coupling diethylenetriaminepentaacetic acid (DTPA) molecules to a partially reduced monoclonal antibody utilizes a malemide modified copolymer of hydroxyethyl methylacrylate and methyl methacrylate (DTPA copolymer) prepared by the group transfer polymerization (GTP) method. An average of 6 DTPA molecules were incorporated per mol maleimeide DTPA copolymer and 1.5 mol maleimide DTPA copolymer per mol antibody. Maleimide DTPA copolymer modified antibody was intramolecularly cross-linked, reduced immunoactivity and had a high in vivo liver uptake.
Comeau
R D
RD
Seragen Inc., Hopkinton, MA 01748.
Liberatore
F A
FA
Cocuzza
A J
AJ
Emond
G Y
GY
Dicker
I B
IB
D000179:Acrylates / Q000138:chemical synthesis*; D000818:Animals; D000911:Antibodies, Monoclonal; D007205:Indium Radioisotopes; D007553:Isotope Labeling / Q000379:methods; D008689:Methacrylates / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D051379:Mice; D004369:Pentetic Acid / Q000138:chemical synthesis* / Q000493:pharmacokinetics / Q000031:analogs & derivatives; D014018:Tissue Distribution
D000179:Acrylates; D000911:Antibodies, Monoclonal; C064249:DTPA copolymer; D007205:Indium Radioisotopes; D008689:Methacrylates; D004369:Pentetic Acid