Identification of fox rabies by a monoclonal antibody directed against nucleocapsid of a street rabies virus.
Hybridomas were prepared by fusion of spleen cells from BALB/c mice immunized with dog rabies isolates from Nigeria with P3x63Ag8 myeloma cells. More than 69 hybridomas secreted antinucleocapsid (antiNC) antibodies when tested with homologous viruses by indirect immunofluorescence. One hybridoma (Z144-88) was found which secreted antiNC antibody that reacted negatively with fox rabies isolates from the Federal Republic of Germany, Switzerland and France and with rabies-related viruses and European bat isolates. It reacted positively with other strains/isolates of rabies virus. It is possible to use this antiNC monoclonal antibody (mab) for the investigation of fox rabies outbreaks in Europe.
Umoh
J U
JU
Department of Veterinary Public Health and Preventive Medicine, Ahmadu Bello University, Zaria, Nigeria.
Cox
J H
JH
Schneider
L G
LG
Meyer
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002213:Capsid / Q000276:immunology*; D004285:Dogs; D005589:Foxes; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D011818:Rabies / Q000175:diagnosis / Q000662:veterinary*; D011820:Rabies virus / Q000276:immunology*; D014758:Viral Core Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D014758:Viral Core Proteins