Molecular analysis of no-on-transient A, a gene required for normal vision in Drosophila.
Mutation of the Drosophila melanogaster gene no-on-transient A (nonA) results in reduced visual acuity, behavior abnormalities, and an electrophysiological defect for which the mutant is named. We mapped the nonA gene genetically to a 20 kb interval within the 14C1,2 region of the X chromosome, isolated this chromosomal region, and used P element-mediated transformation to delimit the nonA gene to a 9 kb region. Analysis of cDNA clones indicates that this region encodes alternatively spliced transcripts encoding protein products of approximately 77 kd that differ only in their C-terminal 35 amino acids. Analysis of mutations generated in vitro in this transcription unit confirm that these transcripts are the products of the nonA gene.
Jones
K R
KR
Department of Molecular and Cell Biology, University of California-Berkeley 94720.
Rubin
G M
GM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis / Q000235:genetics; D004330:Drosophila / Q000235:genetics* / Q000502:physiology; D005796:Genes / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D009154:Mutation; D011506:Proteins / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D014158:Transcription, Genetic; D014170:Transformation, Genetic; D014785:Vision, Ocular / Q000502:physiology*; D014960:X Chromosome / Q000032:analysis / Q000648:ultrastructure
D011506:Proteins; D004247:DNA