title: Glass ionomer and composite resin cements: effects on oral cells.
abstract: Because the cement interfaces of restorations can approximate the periodontium, it is critical to determine the biocompatibility of cements. In this study, the cytotoxic potential of resin luting agents on cultures of gingival fibroblasts and oral epithelial cells was evaluated for direct microscopic cytotoxicity, cell morbidity, impaired adherence, and inhibition of macromolecular synthesis. Visible effects ranged from severe toxicity with inadequately polymerized composite resin to no detectable morphologic cell damage by a glass ionomer cement, but inhibition of protein and RNA synthesis varied with the material and cell type. The glass ionomer cement demonstrated no morphologic damage, but exhibited inhibition of macromolecular synthesis in gingival fibroblasts. These results confirmed that in vitro metabolic assays are appropriate for examining the biologic effects of materials.
authors.affiliation: Medical College of Georgia, School of Dentistry, Augusta.
authors.forename: W F
authors.initials: WF
authors.lastname: Caughman
authors.forename: G B
authors.initials: GB
authors.lastname: Caughman
authors.forename: W T
authors.initials: WT
authors.lastname: Dominy
authors.forename: G S
authors.initials: GS
authors.lastname: Schuster
mesh_terms: D002478:Cells, Cultured; D003188:Composite Resins / Q000633:toxicity*; D003738:Dental Cements / Q000633:toxicity*; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D005881:Gingiva / Q000166:cytology / Q000187:drug effects*; D005899:Glass Ionomer Cements / Q000633:toxicity*; D006801:Humans; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis
chemical_list: D003188:Composite Resins; D003738:Dental Cements; D005899:Glass Ionomer Cements; D012333:RNA, Messenger