title: Quantitative determination of terbutaline and orciprenaline in human plasma by gas chromatography/negative ion chemical ionization/mass spectrometry.
abstract: A method for the determination of unconjugated terbutaline and orciprenaline in human plasma is described. The assay is based on stable isotope dilution gas chromatography/negative ion chemical ionization/mass spectrometry. An inexpensive and rapid method for preparation of stable isotope labelled analogues as well as their use in quantitative gas chromatography/mass spectrometry is shown. A highly efficient sample work-up procedure with product recoveries of more than 95% is presented. The method developed permits quantitative measurement of terbutaline and orciprenaline in human plasma down to 100 pg ml-1, using 1 ml of sample. Plasma levels of terbutaline after oral administration of 5 mg of terbutaline sulphate were estimated.
authors.affiliation: Univ. Kinderklinik, Dept. Mass Spectrometry, Graz, Austria.
authors.forename: H J
authors.initials: HJ
authors.lastname: Leis
authors.forename: H
authors.initials: H
authors.lastname: Gleispach
authors.forename: V
authors.initials: V
authors.lastname: Nitsche
authors.forename: E
authors.initials: E
authors.lastname: Malle
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D066298:In Vitro Techniques; D009921:Metaproterenol / Q000097:blood*; D013726:Terbutaline / Q000097:blood*
chemical_list: D009921:Metaproterenol; D013726:Terbutaline