DNA analysis using polymerase chain reactions in the families of children with cystic fibrosis.
The results of our experience in DNA analysis in cystic fibrosis families using polymerase chain reaction (PCR) is reported. Of the first 10 families studied, seven were fully informative using the probe KM19 and PCR. In the remaining three families, Southern blotting analysis and the probe pXV2c were required before full information was obtained in two and partial information in the other family. The methods used, the current place of PCR and the clinical relevance of DNA analysis are discussed.
Sullivan
M J
MJ
Department of Paediatrics, Christchurch Hospital.
Myles
T
T
Murphy
J
J
George
P
P
Dawson
K P
KP
D015139:Blotting, Southern; D002648:Child; D003550:Cystic Fibrosis / Q000235:genetics*; D004247:DNA / Q000032:analysis*; D015342:DNA Probes; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D010375:Pedigree; D016133:Polymerase Chain Reaction
D015342:DNA Probes; D004247:DNA