title: Peroxidative modification of phospholipids in myocardial membranes.
abstract: Rat heart myocardial membranes exposed to the free radical generating system, Fe2+/ascorbate, undergo lipid peroxidation as evidenced by the accumulation of thiobarbituric acid-reactive substances, loss of polyunsaturated fatty acids from phospholipids, and formation of conjugated dienes and fluorescent substances. In addition, the treated membranes exhibit a dramatic decrease in extractable phospholipids. This decrease is even more pronounced in individual phospholipid classes isolated by high-performance liquid chromatography. The decrease in lipid phosphorus under oxidant stress is accompanied by an increase in the phosphorus content of the aqueous phase after Folch extraction and by an even greater increase of phosphorus in the protein residue. In addition, increased amounts of saturated and monounsaturated fatty acyl groups are found in the protein residue of Fe2+/ascorbate-treated membranes. Extraction of the oxidant-treated membranes with acidic solvents does not enhance the recovery of phospholipids and neither does treatment with detergents, trypsin, and chymotrypsin prior to lipid extraction. However, treatment with the bacterial protease, Pronase, markedly enhances the recovery of phospholipids from the peroxidized membranes. These results indicate that membrane phospholipids undergoing free radical-induced peroxidation may form lipid-protein adducts, which renders them inextractable with lipid solvents.
authors.affiliation: Hormel Institute, University of Minnesota, Austin 55912.
authors.forename: N L
authors.initials: NL
authors.lastname: Parinandi
authors.forename: B K
authors.initials: BK
authors.lastname: Weis
authors.forename: V
authors.initials: V
authors.lastname: Natarajan
authors.forename: H H
authors.initials: HH
authors.lastname: Schmid
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D005296:Ferrous Compounds / Q000494:pharmacology; D005609:Free Radicals; D007700:Kinetics; D015227:Lipid Peroxidation; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D010743:Phospholipids / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D005296:Ferrous Compounds; D005609:Free Radicals; D008563:Membrane Lipids; D010743:Phospholipids; D001205:Ascorbic Acid; C029451:ferrous chloride