title: Diagnosis of cardiac allograft rejection by the detection of circulating plasma cardiac myosin light chains.
abstract: Twenty adult male Japanese monkeys of the species Macaca fuscata were randomly paired and subjected to heterotopic cardiac transplantation performed by the Ono-Lyndsey method. Without immunosuppression, graft survival ranged between 8 and 27 days, with a mean survival of 14 days. Plasma cardiac myosin light chains were measured by radioimmunoassay, which showed transient increases in myosin levels just following transplantation. Three hearts showed high values at this period and stopped beating when the myosin levels decreased (type 1). The other 7 hearts showed low myosin values after transient increases and 5 of them were rejected with a preceding reincrease in the myosin levels (type 2). Pathological study revealed myocardial necrosis, perivascular cuffing of mononuclear cells and/or neutrophils and/or plasma cells in the type 1 hearts. Measurement of the plasma myosin light chain level was therefore revealed to be of great value in the monitoring of cardiac allograft rejection.
authors.affiliation: Department of Thoracic Surgery, Faculty of Medicine, University of Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kawauchi
authors.forename: Y
authors.initials: Y
authors.lastname: Yazaki
authors.forename: T
authors.initials: T
authors.lastname: Oka
authors.forename: H
authors.initials: H
authors.lastname: Okabe
authors.forename: M
authors.initials: M
authors.lastname: Mathison
authors.forename: J
authors.initials: J
authors.lastname: Nakajima
authors.forename: O
authors.initials: O
authors.lastname: Morizuki
authors.forename: G
authors.initials: G
authors.lastname: Kawaguchi
authors.forename: K
authors.initials: K
authors.lastname: Koseni
authors.forename: M
authors.initials: M
authors.lastname: Takeda
mesh_terms: D000818:Animals; D006084:Graft Rejection; D006085:Graft Survival; D006323:Heart Arrest / Q000097:blood; D016027:Heart Transplantation / Q000379:methods / Q000473:pathology; D008251:Macaca; D008297:Male; D009206:Myocardium / Q000473:pathology; D009218:Myosins / Q000097:blood*; D009336:Necrosis; D011897:Random Allocation
chemical_list: D009218:Myosins