title: Increased collagen-linked fluorescence in skin of young patients with type I diabetes mellitus.
abstract: Our objective was to determine whether the fluorescence of skin collagen, which may reflect the accumulation of advanced glycosylation end products, is increased in young patients with type I (insulin-dependent) diabetes. Our study design was a cross-sectional case-control study in a referral-based diabetic clinic in an academic hospital. Study subjects comprised a convenience sample of 18 type I diabetic patients aged 17-30 yr and 8 age-matched healthy control subjects. The fluorescence of collagen was measured in skin biopsy material. Collagen-linked fluorescence (CLF) was increased in diabetic patients (mean 10.5 [range 5.8-15.8] U/mg) compared with control subjects (7.6 [5.6-10.1] U/mg, P less than 0.02). In diabetic patients, CLF was related to age (r = 0.581) and duration of diabetes (r = 0.697) but not concentration of glycosylated hemoglobin (r = 0.082). Partial correlation analysis demonstrated that duration of diabetes is the main factor determining the fluorescence of collagen in these patients. There was a relationship between CLF and presence of diabetic retinopathy after the data were adjusted for patient age and duration of diabetes (P = 0.023). Increased fluorescence of skin collagen can be detected in young type I diabetic patients and is primarily related to duration of diabetes.
authors.affiliation: Department of Pathological Biochemistry, Western Infirmary/Gartnavel General Hospital, Glasgow, Scotland.
authors.forename: M H
authors.initials: MH
authors.lastname: Dominiczak
authors.forename: J
authors.initials: J
authors.lastname: Bell
authors.forename: N H
authors.initials: NH
authors.lastname: Cox
authors.forename: D C
authors.initials: DC
authors.lastname: McCruden
authors.forename: S K
authors.initials: SK
authors.lastname: Jones
authors.forename: A Y
authors.initials: AY
authors.lastname: Finlay
authors.forename: I W
authors.initials: IW
authors.lastname: Percy-Robb
authors.forename: B M
authors.initials: BM
authors.lastname: Frier
mesh_terms: D000328:Adult; D001706:Biopsy; D003094:Collagen / Q000032:analysis*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000473:pathology*; D005260:Female; D005453:Fluorescence; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D008297:Male; D012016:Reference Values; D012867:Skin / Q000166:cytology / Q000473:pathology*
chemical_list: D003094:Collagen; D006442:Glycated Hemoglobin