Effect of radiation and other cytotoxic agents on the growth of cells cultured from normal and tumor tissues from the female genital tract.
A technique is presented which allows the response of human gynecological tissue to radiation and cytotoxic drugs to be assessed using a tissue culture explant system. The technique is simple to use and gives results in line with those obtained for human tissues by more complex culture methods. Data are presented showing how the explant technique developed by the group for other tissues can be adapted to yield acceptable results for normal tissue response to radiation. The potential of the technique for use in predictive testing of individual tumor response is then assessed in five cases of gynecological malignancy. It is clear that variations in sensitivity to different radio- and chemotherapy agents and combinations can be detected. The results obtained require clinical validation and it is hoped that this will come over the next few years from evaluation of patient response to treatment using individually optimized, rather empirical therapy.
Mothersill
C
C
Saint Luke's Hospital, Rathgar, Dublin, Ireland.
Seymour
C B
CB
Bonnar
J
J
D000230:Adenocarcinoma / Q000473:pathology; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D046508:Culture Techniques / Q000379:methods; D005260:Female; D005833:Genital Neoplasms, Female / Q000473:pathology*; D005836:Genitalia, Female / Q000473:pathology*; D006801:Humans; D010051:Ovarian Neoplasms / Q000473:pathology; D011553:Pseudomyxoma Peritonei / Q000473:pathology; D012016:Reference Values; D012509:Sarcoma / Q000473:pathology; D002583:Uterine Cervical Neoplasms / Q000473:pathology; D014594:Uterine Neoplasms / Q000473:pathology
D000970:Antineoplastic Agents