[Aperiodic oscillations of the lipid bilayer potential during enzymatic hydrolysis of the permeating substrate].
The experimental system contained a solution with substrate ATEE-ether, a planar bilayer lipid membrane doped with a protonophore and solution with proteolytic enzyme. Oscillations of the electrical potential on the membrane were shown. The magnitude of the potential was proportional to the rate of the reaction under these conditions. This system may prove useful for the study of the peculiarities of enzyme kinetics complicated by the membrane.
Antonenko
Iu N
IuN
Khakimov
S V
SV
D006868:Hydrolysis; D008051:Lipid Bilayers / Q000378:metabolism*; D008564:Membrane Potentials; D013379:Substrate Specificity; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
D008051:Lipid Bilayers; D014443:Tyrosine; C007651:ethyl N-alpha-acetyl-tyrosinate
Aperiodicheskie kolebaniia potentsiala na bisloĭnoĭ lipidnoĭ membrane pri fermentativnom gidrolize pronikaiushchego substrata.