Simultaneous measurements of endogenous and deuterium-labelled tracer variants of androstenedione and testosterone by capillary gas chromatography-mass spectrometry.
A capillary gas chromatographic-mass spectrometric method for the simultaneous determination of androstenedione and testosterone in human plasma using [19,19,19-2H3]androstenedione and [19,19,19-2H3]testosterone as internal standards is described. For calculation of plasma androstenedione and testosterone, peak heights were measured by selected-ion monitoring of the molecular ions of the heptafluorobutyryl derivatives of androstenedione and [2H3]androstenedione (m/z 482 and 485) and of testosterone and [2H3]testosterone (m/z 680 and 683). The isotope dilution method needed no complex corrections for contributions and provides a sensitive and reliable technique with good accuracy, precision and reproducibility.
Furuta
T
T
Tokyo College of Pharmacy, Japan.
Kusano
K
K
Kasuya
Y
Y
D000735:Androstenedione / Q000097:blood*; D002138:Calibration; D003903:Deuterium; D005466:Fluorocarbons / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods* / Q000592:standards; D006801:Humans; D013739:Testosterone / Q000097:blood*
D005466:Fluorocarbons; C007924:heptafluorobutyric anhydride; D013739:Testosterone; D000735:Androstenedione; D003903:Deuterium