Proliferation and metabolic significance of peroxisomes in Candida boidinii during growth on D-alanine or oleic acid as the sole carbon source.
We have studied the induction of peroxisomes in the methylotrophic yeast Candida boidinii by D-alanine and oleic acid. The organism was able to utilize each of these compounds as the sole carbon source and grew with growth rates of mu = 0.20 h-1 (on D-alanine) or mu = 0.43 h-1 (on oleic acid). Growth was associated with the development of many peroxisomes in the cells. On D-alanine a cluster of tightly interwoven organelles was observed which made up 6.3% of the cytoplasmic volume and were characterized by the presence of D-amino acid oxidase and catalase. On oleic acid rounded to elongated peroxisomes were dominant which were scattered throughout the cytoplasm. These organelles contained increased levels of beta-oxidation enzymes; their relative volume fraction amounted 12.8% of the cytoplasmic volume.
Sulter
G J
GJ
Department of Microbiology, University of Groningen, Haren, The Netherlands.
Waterham
H R
HR
Goodman
J M
JM
Veenhuis
M
M
D000409:Alanine / Q000378:metabolism*; D002175:Candida / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure*; D002458:Cell Fractionation; D003470:Culture Media; D008830:Microbodies / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism
D003470:Culture Media; D009829:Oleic Acids; D019301:Oleic Acid; D010088:Oxidoreductases; D000409:Alanine