Sensitivity of spontaneously epileptic rats to external stimuli that induce seizures.
The sensitivity of spontaneously epileptic rats (SER), double homozygotes of zitter and tremor mutations, to external stimuli that induce seizures was studied in comparison with tremor (tm/tm) and zitter (zi/zi) rats, and with normal Kyo: Wistar and F 344/N rats. Touching their body, a blinking light (1200 lux, 1 sec interval) or a big sound (8 and 12 kHz, 95 dB) induced tonic extension only in the SER. The response frequency was 22 to 44% at 9 weeks of age and 75 to 100% at 13 weeks of age. Electric stimulus at 30 mA induced tonic-clonic convulsions in all Kyo: Wistar and F 344/N rats. At 20 mA the incidence of seizures decreased with age, from 100% at 5 weeks of age to 33% at 13 weeks of age in Kyo: Wistar rats and from 100 to 71% in F344/N rats. In SER, 10-mA stimuli induced tonic extension at 9 and 13 weeks of age, and 20 and 30 mA induced tonic convulsions, generalized or partial convulsions, and wild jumping or running episodes at 5, 9 and 13 weeks of age. At 30 mA, the incidence of convulsive seizures decreased with age in both tremor (tm/tm) and zitter (zi/zi) rats. Apparently external stimuli acted as simple triggers in the induction of tonic extension, since characteristic tonic extension is induced in the SER by each of the stimuli used in the present study, and induced convulsions closely resembled spontaneously occurring convulsions. The threshold of external stimuli in the induction of tonic extension became lower with aging in the SER, indicating that they are appropriate models for evaluating anticonvulsant drugs, as reported previously.
Asano
Y
Y
Safety Research Laboratory, Tanabe Seiyaku Co., Ltd., Osaka, Japan.
Okaniwa
A
A
Serikawa
T
T
Yamada
J
J
D000161:Acoustic Stimulation; D000375:Aging / Q000502:physiology; D000818:Animals; D004558:Electric Stimulation; D004827:Epilepsy / Q000503:physiopathology*; D008297:Male; D010775:Photic Stimulation; D010812:Physical Stimulation; D051381:Rats; D011916:Rats, Inbred F344; D011921:Rats, Inbred WKY; D011922:Rats, Mutant Strains; D012640:Seizures / Q000503:physiopathology*