Identification of Frankia strains in nodules by hybridization of polymerase chain reaction products with strain-specific oligonucleotide probes.
A set of oligonucleotides has been developed to study the competitivity of two Frankia strains in the nodulation of the roots of two host plant species: Alnus glutinosa and Alnus incana. Two 20 mer-oligonucleotides, complementary to highly conserved sequences inside the nifH gene, were used as primers for the polymerase chain reaction (PCR) system in order to amplify microsymbiont DNA extracted from actinorhizae. PCR products were analyzed using two strain-specific 15-mer oligonucleotides identified in the amplified region. Hybridization data indicate that strain ACoN24d is more competitive than strain ArI3 in the nodulation of both hosts.
Simonet
P
P
Laboratoire de Biologie des Sols, U.A. CNRS 697, Université Lyon 1, Villeurbanne, France.
Normand
P
P
Moiroud
A
A
Bardin
R
R
D000192:Actinomycetales / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D015139:Blotting, Southern; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D010944:Plants; D016133:Polymerase Chain Reaction; D013045:Species Specificity
D004269:DNA, Bacterial; D015345:Oligonucleotide Probes