Chemical synthesis and characteristics of a hybrid phage T5-lac promoter.
A new P1O1R9 inducible promoter (where P1 is a promoter sequence analogous to that of the phage T5 early promoter; O1 is lac-operator; and R9 is a ribosome binding site) was synthesized. We studied the efficiency of this promoter in controlling and inducible gene expression using two model genes: human calcitonin tetrameric (hCT[4]) and human interferon alpha 1 (hIFN alpha 1). The synthetic lac-operator O1 was found to repress P1 activity in media free of lac-operon inducers which was derepressed in the presence of IPTG. The levels of expression of both genes (evaluated by quantitating mRNA and protein) in the presence of lac-inducers were close to those obtained with the P1R9 constitutive promoter.
Ivanov
I
I
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
Rommens
J
J
Sarafova
A
A
Maximova
V
V
Usheva
A
A
Bardarov
S
S
Papageorgiou
A
A
Jay
E
E
D001483:Base Sequence; D002116:Calcitonin / Q000235:genetics*; D003001:Cloning, Molecular; D015967:Gene Expression Regulation, Viral; D006801:Humans; D007370:Interferon Type I / Q000235:genetics*; D007700:Kinetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D013604:T-Phages / Q000235:genetics*
D007370:Interferon Type I; D002116:Calcitonin