[Interaction of prostaglandins and fatty acids with native and acetaldehyde-alkylated proteins].
Using intrinsic and probe fluorescence, microcalorimetry and isotopic methods, the interactions of prostaglandins (PG) E2 and F2 alpha and some fatty acids with native and alkylated proteins (human serum albumin (HSA) and rat liver plasma membrane PG receptors), were studied. The fatty acid and PG interactions with human serum albumin (HSA) resulted in effective quenching of fluorescence of the probe, 1.8-anilinonaphthalene sulfonate (ANS), bound to the protein. Fatty acids competed with ANS for the binding sites; the efficiency of this process increased with an increase in the number of double bonds in the fatty acid molecule. PG induced a weaker fluorescence quenching of HSA-bound ANS and stabilized the protein molecule in a lesser degree compared to fatty acids. The sites of PG E2 and F2 alpha binding did not overlap with the sites of fatty acid binding on the HSA molecule. Nonenzymatic alkylation of HSA by acetaldehyde resulted in the abnormalities of binding sites for fatty acids and PG. Modification of the plasma membrane proteins with acetaldehyde sharply diminished the density of PG E2 binding sites without changing the association constants. Alkylation did not interfere with the parameters of PG F2 alpha binding to liver membrane proteins.
Buko
V U
VU
Zavodnik
I B
IB
Lapshina
E A
EA
D000079:Acetaldehyde / Q000378:metabolism*; D000478:Alkylation; D000818:Animals; D015237:Dinoprost / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D005456:Fluorescent Dyes; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism; D011453:Prostaglandins / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D012709:Serum Albumin / Q000378:metabolism
D005227:Fatty Acids; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D008565:Membrane Proteins; D011453:Prostaglandins; D011506:Proteins; D012709:Serum Albumin; D015237:Dinoprost; D000079:Acetaldehyde; D015232:Dinoprostone
Vzaimodeĭstvie prostaglandinov i zhirnykh kislot s nativnymi i alkilirovannymi atsetal'degidom belkami.