title: Computerized three-dimensional reconstructions of serial sections in electron microscopy.
abstract: To improve reconstructive 3D electron microscopy novel methods are discussed to represent and process serial section images in a cuberille environment. This includes the analysis of the transfer characteristics of the image detection system, the use of laser-induced fiducials for deformation correction and alignment, the control of section thickness by EELS and the use of ESI to image thick sections.
authors.affiliation: Institute of Anatomy and Cytobiology, Justus-Liebig-University, Giessen, Fed. Rep. of Germany.
authors.forename: A
authors.initials: A
authors.lastname: Kriete
authors.forename: G
authors.initials: G
authors.lastname: Magdowski
mesh_terms: D000465:Algorithms; D000818:Animals; D003196:Computer Graphics; D005583:Fourier Analysis; D007091:Image Processing, Computer-Assisted; D007668:Kidney / Q000648:ultrastructure*; D008168:Lung / Q000648:ultrastructure*; D051379:Mice; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D051381:Rats; D013057:Spectrum Analysis