Neurofilament gene expression in transgenic mice.
1. DNA fragments that include the human neurofilament NF-L gene was found to be correctly expressed in the majority of neurons in transgenic mice. 2. The NF-L transgene product, which is detectable in situ with a species-specific monoclonal antibody, provides a powerful genotype marking system applicable to developmental and regeneration studies of the mammalian nervous system. 3. The proximal 5'-flanking region of the NF-L gene is sufficient to direct expression of a heterologous gene in the mouse nervous system.
Julien
J P
JP
Institut du Cancer de Montreal, Centre Hospitalier Notre-Dame, Canada.
Beaudet
L
L
Tretjakoff
I
I
Peterson
A
A
D000818:Animals; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D015870:Gene Expression; D005796:Genes; D006801:Humans; D007381:Intermediate Filament Proteins / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons / Q000502:physiology; D012045:Regulatory Sequences, Nucleic Acid / Q000502:physiology; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D007381:Intermediate Filament Proteins; D015500:Chloramphenicol O-Acetyltransferase