title: An ELISA for quantitation of tetanus toxin.
abstract: A sandwich ELISA for quantitation of tetanus toxin was devised using commercially available horse antitetanus antiserum and its HRP-conjugate. With commercial toxoid as a standard the assay had a lower sensitivity of 100 ng/ml and was linear up to 100 micrograms/ml. In terms of Lf equivalents the ELISA assay was linear from 0.02 to 20 Lf/ml. The Lf activity in 20 preparations of tetanus toxin as determined by ELISA was comparable to the activity measured by conventional flocculation tests (r = 0.987).
authors.affiliation: Department of Microbiology, All India Institute of Medical Sciences, New Delhi.
authors.forename: A
authors.initials: A
authors.lastname: Pal
authors.forename: R
authors.initials: R
authors.lastname: Kumar
authors.forename: B L
authors.initials: BL
authors.lastname: Jailkhani
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D005427:Flocculation; D011237:Predictive Value of Tests; D013744:Tetanus Toxin / Q000032:analysis*
chemical_list: D013744:Tetanus Toxin