Lymphoblastic crisis of chronic myelogenous leukemia. Hand mirror variant.
We present, to our knowledge, the first extensively studied case of lymphoid L2 blast crisis of chronic myelogenous leukemia with a hand mirror cell (HMC) variant. Special stains revealed the leukemic cells to be terminal deoxynucleotidyl transferase positive by immunofluorescence and cytochemically positive for alpha-naphthyl acetate esterase and acid phosphatase (diffuse granular). Immunophenotyping identified the major leukemic cell population as B-cells that expressed CD10+, CD19+, and HLA-DR+. It was not possible to separate the HMC and the non-HMC leukemic population by gating various cell populations, dual staining, cytochemistry, or by terminal deoxynucleotidyl transferase. Gene rearrangements were observed in both Ig heavy-chain alleles and one T-cell antigen receptor gamma-subunit allele. The rearrangements occupied all of the cells, indicating that the HMC and non-HMC were of a common clonal origin. The patient had a mosaic karyotype, with 90% of the cells having t(9;22), t(8;14), and t(9;15) translocations, an additional chromosome 8, and deleted chromosomes 9 and 15. Antibodies to simian sarcoma-associated virus and baboon endogenous virus were isolated in the patient's peripheral blood plasma.
Kowal-Vern
A
A
Department of Pathology, Loyola University Medical Center, Maywood, Ill 60153.
Birdsong
B A
BA
Dizikes
G
G
Radvany
R
R
Desai
S N
SN
Schumacher
H
H
D000951:Antigens, Neoplasm / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology; D001752:Blast Crisis / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D015153:Blotting, Western; D001853:Bone Marrow / Q000473:pathology; D002875:Chromosomes / Q000032:analysis; D004900:Erythroblasts / Q000276:immunology / Q000473:pathology; D015321:Gene Rearrangement; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D010641:Phenotype
D000951:Antigens, Neoplasm