Computerized slow-staged freezing of semen from men with testicular tumors or Hodgkin's disease preserves sperm better than standard vapor freezing.
Two methods of freezing semen taken from patients with testicular tumors or Hodgkin's disease before treatment were compared. Many patients already have semen abnormalities, so an optimal method is extremely important. Ejaculates from 8 patients with testicular tumors and 20 with Hodgkin's disease were frozen by fast-freezing or by slow-staged freezing. Effects of motility, viability, and swelling after thawing were significantly impaired with both methods. However, cryosurvival was better after slow- than fast-freezing: motility 24% +/- 12.4% versus 15% +/- 11.2%; viability 24.1% +/- 11.4% versus 17.3% +/- 10.4%, swelling 33.3% +/- 11% versus 27.6% +/- 12.8%. The effects were equal for normal and abnormal sperm. Sperm from tumor patients should be frozen by slow-staged freezing method in spite of the higher cost and longer time.
Ragni
G
G
University of Milan, Italy.
Caccamo
A M
AM
Dalla Serra
A
A
Guercilena
S
S
D003201:Computers; D015925:Cryopreservation / Q000009:adverse effects / Q000379:methods*; D006689:Hodgkin Disease; D006801:Humans; D007248:Infertility, Male / Q000209:etiology; D008297:Male; D008875:Middle Aged; D012661:Semen / Q000032:analysis; D012662:Semen Preservation / Q000379:methods*; D013081:Sperm Motility; D013736:Testicular Neoplasms