title: Intraocular irrigating solutions and permeability of the blood-aqueous barrier.
abstract: The effects of intraocular irrigating solutions on the blood-aqueous barrier were studied using rabbits injected with fluorescein isothiocyanate-labeled rabbit albumin. Two days after the injection, the anterior chamber was perfused for 121 minutes with one of the following solutions: normal saline, a commercially available citrate-acetate bicarbonate solution, or a glucose glutathione bicarbonate solution; the amount of fluorescein isothiocyanate-labeled rabbit albumin collected in the outflowing perfusate standardized to its plasma concentration was adopted as a blood-aqueous barrier permeability index. Of the three solutions, normal saline showed the highest permeability index and glucose glutathione bicarbonate solution showed the lowest. The effects of oxidized glutathione on the blood-aqueous barrier were also studied, using solutions containing various concentrations of oxidized glutathione. The permeability index for the solution containing 0.3 mmol/L of oxidized glutathione was significantly smaller than that for the solution without oxidized glutathione, suggesting that oxidized glutathione has a beneficial effect on the integrity of the blood-aqueous barrier.
authors.affiliation: Department of Ophthalmology, University of Tokyo, School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Araie
authors.forename: E
authors.initials: E
authors.lastname: Shirasawa
authors.forename: T
authors.initials: T
authors.lastname: Ohashi
mesh_terms: D000085:Acetates / Q000378:metabolism; D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism / Q000502:physiology*; D001639:Bicarbonates / Q000378:metabolism; D001693:Biological Transport, Active; D002951:Citrates / Q000378:metabolism; D005456:Fluorescent Dyes; D005978:Glutathione / Q000031:analogs & derivatives / Q000378:metabolism; D019803:Glutathione Disulfide; D009883:Ophthalmic Solutions; D010539:Permeability; D011817:Rabbits; D012965:Sodium Chloride / Q000378:metabolism; D007507:Therapeutic Irrigation
chemical_list: D000085:Acetates; D001639:Bicarbonates; D002951:Citrates; D005456:Fluorescent Dyes; D009883:Ophthalmic Solutions; D012965:Sodium Chloride; D005978:Glutathione; D019803:Glutathione Disulfide