Reactive oxygen intermediates metabolizing enzymes in Ancylostoma ceylanicum and Nippostrongylus brasiliensis.
Adult worms of Ancylostoma ceylanicum and Nippostronglyus brasiliensis were found to possess an active system for the detoxification of reactive oxygen intermediates. Xanthine oxidase, which is known to produce superoxide anion, was detected in both the nematode parasites in significant activities. Superoxide anion, thus produced, may quickly be eliminated by superoxide dismutase. Both parasites also exhibited the presence of catalase, peroxidase, and glutathione peroxidase for efficient removal of hydrogen peroxide. Glutathione reductase and glucose-6-phosphate dehydrogenase were, however, detected in low levels of activities. Endowment of A. ceylanicum and N. brasiliensis with these antioxidant enzymes, therefore, enables them to evade the host's effector mechanism for their survival. Superoxide dismutase of both these nematodes showed marked inhibition by KCN and, hence, the enzyme appears to be of copper-zinc type.
Batra
S
S
Division of Biochemistry, Central Drug Research Institute, Lucknow, India.
Singh
S P
SP
Gupta
S
S
Katiyar
J C
JC
Srivastava
V M
VM
D000722:Ancylostoma / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D006224:Cricetinae; D004798:Enzymes / Q000378:metabolism*; D005609:Free Radicals; D007422:Intestines / Q000469:parasitology*; D008297:Male; D009559:Nippostrongylus / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D051381:Rats
D000975:Antioxidants; D004798:Enzymes; D005609:Free Radicals; D010100:Oxygen