Influence of complement on sperm motility.
Isolated sperm from normo-, oligo- and astheno-spermic men were incubated for 20 h in medium supplemented with 8% heat-inactivated or untreated human serum, and in medium with heated or untreated serum deficient in complement factor C3. Before and after incubation, sperm motility was assessed by means of a computer-assisted semen analyser. The results did not show significant differences between the motility of sperm incubated in heated or untreated serum. It is concluded that heating of homologous serum is not necessary for preserving sperm motility and in some cases may even be disadvantageous.
Vogelpoel
F R
FR
Department of Clinical Bacteriology, Academic Hospital of Utrecht, The Netherlands.
Van Kooij
R J
RJ
Te Velde
E R
ER
Verhoef
J
J
D001769:Blood; D003176:Complement C3 / Q000494:pharmacology*; D005260:Female; D005307:Fertilization in Vitro; D006358:Hot Temperature; D006801:Humans; D008297:Male; D013081:Sperm Motility / Q000187:drug effects*
D003176:Complement C3