title: Purification and immunohistochemical study of actin in mitochondrial matrix.
abstract: Actin was purified to apparent homogeneity from the matrix of ultra-pure mitochondria of rat livers by DNase-I affinity chromatography and HPLC gel filtration. The mitochondrial actin was immunologically identified by an anti-actin antibody, and its apparent molecular weight was 43 KDa, as determined by SDS-polyacrylamide gel electrophoresis. The immunohistochemical study revealed the localization of the mitochondrial actin in the matrix space and on the internal surface of inner membrane. The actin fraction eluted from a DNase-I column by KCl-EGTA solution underwent polymerization and bundling in vitro.
authors.affiliation: Department of Physiology, Kagawa Medical School, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Etoh
authors.forename: H
authors.initials: H
authors.lastname: Matsui
authors.forename: M
authors.initials: M
authors.lastname: Tokuda
authors.forename: T
authors.initials: T
authors.lastname: Itano
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
authors.forename: O
authors.initials: O
authors.lastname: Hatase
mesh_terms: D000199:Actins / Q000032:analysis / Q000302:isolation & purification*; D000818:Animals; D001704:Biopolymers; D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D003850:Deoxyribonuclease I; D015151:Immunoblotting; D008854:Microscopy, Electron; D008930:Mitochondria, Liver / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013367:Submitochondrial Particles / Q000032:analysis*
chemical_list: D000199:Actins; D001704:Biopolymers; D003850:Deoxyribonuclease I