title: A contiguous network of dendritic antigen-presenting cells within the respiratory epithelium.
abstract: This study utilises a simple technique to section airway epithelium in a plane parallel to the basement membrane, thus providing a unique plan view of the intra-epithelial cell populations. Immunoperoxidase staining of these tissue sections for class II major histocompatibility complex Ia antigen reveals a virtually contiguous network of Ia-positive dendritic cells (DC) within the epithelium. These DC are shown to be capable of binding inhaled antigens in vivo in a form suitable for presentation to T cells. The strategic location of these cells and the fact that they account for virtually all staining in the airway epithelium during the steady state is convincing evidence that the DC network functions as the 'first line of defence' in surveillance for inhaled antigens and further suggests a major role for the intra-epithelial DC in allergic and infectious disease(s) in the respiratory tract.
authors.affiliation: Clinical Immunology Research Unit, Children's Medical Research Foundation, Princess Margaret Hospital, Perth, Australia.
authors.forename: P G
authors.initials: PG
authors.lastname: Holt
authors.forename: M A
authors.initials: MA
authors.lastname: Schon-Hegrad
authors.forename: J
authors.initials: J
authors.lastname: Oliver
authors.forename: B J
authors.initials: BJ
authors.lastname: Holt
authors.forename: P G
authors.initials: PG
authors.lastname: McMenamin
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000166:cytology*; D003713:Dendritic Cells / Q000166:cytology*; D004848:Epithelium / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D051381:Rats; D011914:Rats, Inbred BN; D014132:Trachea / Q000276:immunology*
chemical_list: D000949:Histocompatibility Antigens Class II