title: Mutagenic and lethal action of polychromatic near-ultraviolet (325-400 nm) on Haemophilus influenzae in the presence of nitrogen.
abstract: The lethal effect of polychromatic near-UV light (325-400 nm) on Haemophilus influenzae was 8 times higher under aerobic than anaerobic irradiation. This light increased the frequency of mutation to novobiocin resistance and ability to utilize protoporphyrin IX. The slope of mutagenic effect at low doses appeared greater for the aerobic than for the anaerobic group. We concluded that polychromatic near-UV mutation of H. influenzae under anaerobic irradiation was caused by direct oxygen-independent action on DNA.
authors.affiliation: Departamento de Bioquímica, Escuela Nacional de Ciencias Biológicas, I.P.N., México City, México.
authors.forename: E
authors.initials: E
authors.lastname: Cabrera-Juárez
authors.forename: M
authors.initials: M
authors.lastname: Espinosa-Lara
mesh_terms: D004352:Drug Resistance, Microbial; D006193:Haemophilus influenzae / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D009152:Mutagenicity Tests; D009675:Novobiocin / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D009675:Novobiocin