Effects of OK-432 activation on the sialidase activities of rat peritoneal macrophages.
Intraperitoneal treatment of rat peritoneal macrophages with OK-432 results in more than 9-fold increase in the activity of ganglioside sialidase, which seems to coincide with the appearance of a cell surface antigen, asialo-GM1. The results of subsequent studies suggest that the ganglioside sialidase is located in the plasma membrane, where the enzyme may be responsible for the formation of asialo-GM1 from GM1. In the macrophages activated with OK-432, sialidase activity toward 4-methylumbelliferyl-N-acetylneuraminic acid (4MU-NeuAc) is also increased. It appears that the 4MU-NeuAc sialidase is intralysosomal and is increased together with other acid hydrolases present in the lysosomes.
Sagawa
J
J
Research Institute for Tuberculosis and Cancer, Tohoku University, Sendai.
Miyagi
T
T
Tsuiki
S
S
D000818:Animals; D001688:Biological Products / Q000494:pharmacology*; D005732:Gangliosides / Q000378:metabolism*; D066298:In Vitro Techniques; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology*; D009439:Neuraminidase / Q000378:metabolism*; D010529:Peritoneal Cavity / Q000166:cytology*; D010844:Picibanil / Q000494:pharmacology*; D051381:Rats
D001688:Biological Products; D005732:Gangliosides; D010844:Picibanil; D009439:Neuraminidase