title: The impact of peritoneal trauma on intra-abdominal fibrinolytic activity, adhesion formation and early embryonic development in a rabbit longitudinal model.
abstract: Intra-abdominal adhesions may interfere with fertility following gynaecological surgery and injury to the peritoneum plays a central role in the pathogenesis. Tissue plasminogen activator and its antagonists play a pivotal role in the intra-abdominal balance between fibrinolysis and adhesion formation. This process may be cycle-dependent in women. In order to establish the impact of the fibrinolytic activity on adhesion formation after a standardized trauma, a rabbit longitudinal model was developed, which allowed the study of possible differences between the periods before and after ovulation. The influence of extra-genital adhesions on early embryonic development was investigated. No cycle-dependent changes in fibrinolytic activity of the peritoneal fluid (PF) or of the serum could be demonstrated. No correlation was found between post-operative adhesion formation and the fibrinolytic activity during surgery. Three weeks after surgery, a significant increase in fibrinolytic activity of the PF was observed. The rank order of sampling is suggested to account for these differences. Extra-genital adhesions did not markedly influence ovulation, ovum pick-up and fertilization in this hormonally controlled rabbit model.
authors.affiliation: Department of Obstetrics and Gynaecology, University Hospital, Maastricht, The Netherlands.
authors.forename: P X
authors.initials: PX
authors.lastname: Bouckaert
authors.forename: J A
authors.initials: JA
authors.lastname: Land
authors.forename: E J
authors.initials: EJ
authors.lastname: Brommer
authors.forename: J J
authors.initials: JJ
authors.lastname: Emeis
authors.forename: J L
authors.initials: JL
authors.lastname: Evers
mesh_terms: D000818:Animals; D001202:Ascitic Fluid / Q000032:analysis; D005314:Embryonic and Fetal Development; D005260:Female; D005306:Fertilization; D005342:Fibrinolysis; D008137:Longitudinal Studies; D010060:Ovulation; D010532:Peritoneal Diseases / Q000209:etiology*; D010537:Peritoneum / Q000293:injuries*; D011817:Rabbits; D000267:Tissue Adhesions / Q000209:etiology