Direct enantiomeric separation of saterinone by high-performance liquid chromatography.
A new method is described for the direct liquid chromatographic separation and determination of the enantiomers of saterinone, 1-[(4-cyano-1,2-dihydro-6-methyl-2-oxopyridinyl-5)-phenoxy]-3-[4-( 2- methoxyphenyl)piperazinyl-1]-propan-2-ol, using a Chiralcel OD column with methanol as eluent and a temperature set to 10 degrees C. Several lots of synthesized enantiomers were analysed for their enantiomeric purity of more than 99%, because of the excellent resolution (Rs = 2.2). Furthermore the enantiomeric ratio was determined in plasma samples after oral and intravenous administration of racemic saterinone.
Rudolph
M
M
Beiersdorf AG, R&D Pharma, Biochemical Department, Hamburg, F.R.G.
D000284:Administration, Oral; D002316:Cardiotonic Agents / Q000008:administration & dosage / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D006801:Humans; D007275:Injections, Intravenous; D010879:Piperazines / Q000008:administration & dosage / Q000097:blood*; D011728:Pyridones / Q000008:administration & dosage / Q000097:blood*
D002316:Cardiotonic Agents; D010879:Piperazines; D011728:Pyridones; C058572:saterinone