title: Phosphorylation of ornithine decarboxylase in intact erythroleukemia cells.
abstract: 32P-labeled ornithine decarboxylase was isolated by immunoprecipitation from murine erythroleukemia cells incubated in a medium containing [32P]ortophosphoric acid. Analysis of immunoprecipitate by SDS-polyacrylamide gel electrophoresis and autoradiography revealed a radiolabeled band, which corresponded to the position of mouse ornithine decarboxylase, phosphorylated in vitro by casein kinase-2. A preparation of casein kinase-2 purified from nuclei of erythroleukemia cells could also phosphorylate mouse ornithine decarboxylase.
authors.affiliation: Dipartimento di Biochimica, Università di Bologna, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Flamigni
authors.forename: S
authors.initials: S
authors.lastname: Marmiroli
authors.forename: F
authors.initials: F
authors.lastname: Meggio
authors.forename: C
authors.initials: C
authors.lastname: Guarnieri
authors.forename: L A
authors.initials: LA
authors.lastname: Pinna
mesh_terms: D000818:Animals; D047388:Casein Kinases; D002467:Cell Nucleus / Q000201:enzymology; D004915:Leukemia, Erythroblastic, Acute / Q000201:enzymology*; D051379:Mice; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D010766:Phosphorylation; D011233:Precipitin Tests; D011494:Protein Kinases / Q000378:metabolism; D011817:Rabbits; D014407:Tumor Cells, Cultured
chemical_list: D011494:Protein Kinases; D047388:Casein Kinases; D009955:Ornithine Decarboxylase