title: cDNA cloning and characterization of ret activated in a human papillary thyroid carcinoma cell line.
abstract: We obtained activated ret cDNAs (retTPC) from a human papillary thyroid carcinoma cell line, TPC-1, and characterized its structure. The nucleotide sequence indicated that the recombination had occurred just upstream of the kinase domain of ret proto-oncogene and that the position, where the conserved sequence of ret proto-oncogene starts in retTPC transcripts, was exactly the same as that of ret-II which we have previously analyzed. Furthermore, a unique 13-glycine stretch, which is also present in a small subunit of the calcium dependent protease, calpain, was detected in the replaced sequence of retTPC. The aberrant tyrosine kinase activity induced by the rearrangement of ret proto-oncogene could be involved in the development of papillary thyroid carcinoma.
authors.affiliation: Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Ishizaka
authors.forename: T
authors.initials: T
authors.lastname: Ushijima
authors.forename: T
authors.initials: T
authors.lastname: Sugimura
authors.forename: M
authors.initials: M
authors.lastname: Nagao
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002291:Carcinoma, Papillary / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D006801:Humans; D008969:Molecular Sequence Data; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes; D015183:Restriction Mapping; D013964:Thyroid Neoplasms / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D004247:DNA