Effect of transforming growth factor beta on fibroblasts in three-dimensional lattice cultures.
Human skin fibroblasts were cultivated in three-dimensional fibrin or collagen lattices, under retracting or non-retracting conditions, and the influence of transforming growth factor beta (TGF beta) was tested. TGF beta stimulated the synthesis of non-collagen protein and of collagen in all the systems. However, only in non-retracting fibrin lattices did it restore a level of protein synthesis comparable to that found in monolayers. The effects of TGF beta greatly depended on the type of substratum and on the presence or absence of retraction.
Coustry
F
F
Laboratory of Biochemistry, CNRS URA 610, University of Reims, France.
Gillery
P
P
Maquart
F X
FX
Borel
J P
JP
D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D011392:Proline / Q000378:metabolism; D011487:Protein Conformation; D015291:Transforming Growth Factors / Q000494:pharmacology*
D015291:Transforming Growth Factors; D003094:Collagen; D011392:Proline