title: A spectrophotometric method for detection of 5'-fluorouracil in plasma.
abstract: A quantitative method using spectrophotometry was devised for the detection of 5'-Fluorouracil (FUra) in plasma. Plasma samples obtained at different time intervals from drug treated rabbits were deproteinised using phenol. The aqueous phase was lyophilised and dissolved in 0.01 N NaOH and absorption was monitored at 305 nm. Levels of FUra in the plasma were calculated by comparing with a standard graph generated from known concentrations of FUra. As low as 1 microgram of the drug per ml of plasma could be detected by this method.
authors.affiliation: Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore.
authors.forename: A M
authors.initials: AM
authors.lastname: Kumar
authors.forename: R
authors.initials: R
authors.lastname: Nayak
mesh_terms: D000818:Animals; D002855:Chromatography, Thin Layer; D005472:Fluorouracil / Q000097:blood*; D008297:Male; D011817:Rabbits; D013057:Spectrum Analysis / Q000379:methods*
chemical_list: D005472:Fluorouracil