X-ray microanalysis of rotavirus-infected mouse intestine: a new concept of diarrhoeal secretion.
Neonatal mice were infected at 7 days of age with rotavirus [epizootic diarrhea of infant mice (EDIM) virus] and killed at 24-h intervals postinfection (PI). Cytoplasmic concentrations of Na, Mg, P, S, Cl, K, and Ca intestinal epithelial cells from infected and age-matched control animals were measured by x-ray microanalysis. In villus tip cells, Ca concentration increased at 24-96 h PI; Na concentration increased at 24-72 h PI; Ca and Na concentrations were near normal by 168 h PI. K concentration decreased 24-72 h PI, and Cl concentration decreased 48-96 h PI. In crypt cells, changes were observed without a discernible pattern: at 96 h PI, Na, Mg, S, and Cl concentrations increased and K concentration decreased; at 120 h PI, the concentrations of all elements except Na and Ca increased. In villus base cells, the mean concentrations of all elements except Ca peaked at 48-72 h PI and at 120 h PI. Na and Cl concentrations increased dramatically in some cells from 48 h PI onward. All the above concentration values were obtained from freeze-dried specimens and expressed in millimoles per kilogram of dry weight. Conversion of a limited number of data, pertaining to villus base cells, from dry weight to wet weight was possible. This conversion revealed that villus base cells in infected animals were more hydrated than corresponding cells from control animals. Also, the Na and Cl concentrations in mmol/kg H2O were significantly higher in villus base cells from infected animals than in those from corresponding controls: 137 +/- 7 versus 38 +/- 4 (Na) and 121 +/- 5 versus 89 +/- 6 (Cl). Wet weight concentrations of other elements were either the same (Mg) or lower (P, S, and K) after infection with virus. From these studies, a new concept of the pathophysiology of rotavirus-induced diarrhoeal secretion is proposed: stimulation of villus base cells to rapid cell division is accompanied by transient accumulation of Na and Cl; excess NaCl is secreted into the lumen, which is the driving force for fluid loss.
Spencer
A J
AJ
Department of Physiology, University of Birmingham, England.
Osborne
M P
MP
Haddon
S J
SJ
Collins
J
J
Starkey
W G
WG
Candy
D C
DC
Stephen
J
J
D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000378:metabolism; D002455:Cell Division; D002712:Chlorides / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D003967:Diarrhea / Q000378:metabolism / Q000382:microbiology / Q000503:physiopathology*; D004577:Electron Probe Microanalysis; D066298:In Vitro Techniques; D007419:Intestinal Secretions / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism / Q000382:microbiology / Q000503:physiopathology*; D008274:Magnesium / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D011188:Potassium / Q000378:metabolism; D012400:Rotavirus Infections / Q000378:metabolism / Q000503:physiopathology*; D012964:Sodium / Q000378:metabolism
D002712:Chlorides; D012964:Sodium; D008274:Magnesium; D011188:Potassium; D002118:Calcium