title: Sensitive and rapid colorimetric immunoenzymometric assay of ferritin in biological samples.
abstract: We describe a rapid and sensitive enzyme-linked immunosorbent assay (ELISA) for quantifying ferritin in human and rat biological fluids. We used chlorophenol red beta-D-galactopyranoside as the colorimetric substrate of beta-galactosidase (EC 3.2.1.23), which is coupled to specific antibodies to either human or rat liver ferritin. The assay is sensitive (detection limit for human assay = 0.58 micrograms/L and for rat assay = 0.37 micrograms/L), accurate (average recovery for human assay = 93% and for rat assay = 92%), and precise (total CVs for human assay = 2.3-12.2% and for rat assay = 5.6-11.3%). The results correlated well with those of an established immunoradiometric technique (r = 0.99691). This assay has a prolonged shelf-life, is inexpensive, and utilizes a stable colorimetric substrate that requires relatively short incubation.
authors.affiliation: Department of Medicine, Royal Brisbane Hospital, Australia.
authors.forename: G A
authors.initials: GA
authors.lastname: Ramm
authors.forename: L R
authors.initials: LR
authors.lastname: Duplock
authors.forename: L W
authors.initials: LW
authors.lastname: Powell
authors.forename: J W
authors.initials: JW
authors.lastname: Halliday
mesh_terms: D000818:Animals; D001322:Autoanalysis; D001646:Bile / Q000032:analysis*; D003124:Colorimetry; D004797:Enzyme-Linked Immunosorbent Assay; D005293:Ferritins / Q000032:analysis* / Q000097:blood; D006801:Humans; D015592:Immunoradiometric Assay; D051381:Rats; D015203:Reproducibility of Results
chemical_list: D005293:Ferritins