Reductive dechlorination of 3-chlorobenzoate is coupled to ATP production and growth in an anaerobic bacterium, strain DCB-1.
Thermodynamic data that the reductive dechlorination of 3-chlorobenzoate is exergonic have led to the hypothesis that this reaction yields biologically useful energy. This hypothesis was tested with strain DCB-1, a dehalogenating bacterium. The organism was grown under strictly anaerobic conditions in vitamin-amended mineral medium with formate plus acetate as electron donor and 3-chlorobenzoate as electron acceptor. The cell yield increased stoichiometrically to the amount of 3-chlorobenzoate dechlorinated. No growth was observed in the absence of 3-chlorobenzoate, or when 3-chlorobenzoate was replaced by benzoate. To obtain further evidence on that energy is derived from dechlorination, 3-chlorobenzoate was added to starved cells. This amendment resulted in an increase in the ATP level of the cells at 10 nmol per mg protein versus 3 nmol per mg protein in non-amended controls. These data indicate that the reductive dehalogenation of chlorinated aromatic compounds can be coupled to a novel type of chemotrophy.
Dolfing
J
J
Department of Biochemistry, University of Groningen, The Netherlands.
D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D001421:Bacteria, Anaerobic / Q000254:growth & development / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002723:Chlorobenzoates / Q000378:metabolism*; D010084:Oxidation-Reduction
D002723:Chlorobenzoates; C036427:3-chlorobenzoic acid; D000255:Adenosine Triphosphate