title: Inducible cytosolic enzyme activity for the production of nitrogen oxides from L-arginine in hepatocytes.
abstract: The in vivo conditions needed for the induction of nitrogen oxide synthesis by hepatocytes were determined. Hepatocytes obtained from rats injected with killed Corynebacterium parvum spontaneously produced NO2(-)+NO3- in culture and were found to contain cytosolic enzyme activity for nitrogen oxide synthesis. The enzyme activity required both L-arginine and NADPH, and was not found in hepatocytes obtained from normal rats or rats injected with lipopolysaccharide (LPS) alone. In contrast, nonparenchymal cells were stimulated to synthesize NO2(-)+NO3- by LPS. These results show the presence of inducible cytosolic enzyme activity for nitrogen oxide synthesis in hepatocytes, which is distinct from nonparenchymal cell NO. synthesis.
authors.affiliation: Department of Neurobiology, Anatomy, and Cell Science, University of Pittsburgh, PA 15261.
authors.forename: T R
authors.initials: TR
authors.lastname: Billiar
authors.forename: R D
authors.initials: RD
authors.lastname: Curran
authors.forename: D J
authors.initials: DJ
authors.lastname: Stuehr
authors.forename: J
authors.initials: J
authors.lastname: Stadler
authors.forename: R L
authors.initials: RL
authors.lastname: Simmons
authors.forename: S A
authors.initials: SA
authors.lastname: Murray
mesh_terms: D000818:Animals; D001120:Arginine / Q000378:metabolism*; D002478:Cells, Cultured; D003600:Cytosol / Q000201:enzymology*; D004790:Enzyme Induction / Q000187:drug effects; D008025:Ligases / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D009249:NADP / Q000494:pharmacology; D009589:Nitrogen Oxides / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D008070:Lipopolysaccharides; D009589:Nitrogen Oxides; D009249:NADP; D001120:Arginine; D008025:Ligases