title: Determination of the enantiomers of suprofen and [2H3]suprofen in plasma by capillary gas chromatography-mass spectrometry.
abstract: A method for the stereoselective assay of the (+)- and (-)-enantiomers of suprofen and [2H3]suprofen in human plasma was developed using gas chromatography-mass spectrometry-selected-ion monitoring. (+/-)-[2H7]Suprofen was used as an internal standard. The method involved diethyl ether extraction and chiral derivatization with S-(-)-1-(naphthyl)ethylamine to form diastereomeric amide. The diastereoisomers were separated on a capillary gas chromatograph-mass spectrometer. Quantitation was achieved by selected-ion monitoring of the quasi-molecular ions of the diastereoisomers. The sensitivity, specificity, accuracy and reproducibility of the method were demonstrated to be satisfactory for application to pharmacokinetic studies of suprofen enantiomers.
authors.affiliation: Tokyo College of Pharmacy, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Shinohara
authors.forename: N
authors.initials: N
authors.lastname: Kirii
authors.forename: H
authors.initials: H
authors.lastname: Tamaoki
authors.forename: H
authors.initials: H
authors.lastname: Magara
authors.forename: S
authors.initials: S
authors.lastname: Baba
mesh_terms: D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D013237:Stereoisomerism; D013496:Suprofen / Q000097:blood*
chemical_list: D013496:Suprofen