title: Identification of DNA ligase I related polypeptides in three different human cells.
abstract: Partial purification of the DNA ligase from three human tissues (liver, thymus and lymphoblasts) revealed that each cell type contains several different polypeptides bearing a DNA ligase I activity. Their apparent molecular weights estimated after SDS PAGE, 130 kDa, 100 kDa and 80 kDa, are in agreement with previous reports. These polypeptides are related by proteolysis to a single higher molecular weight protein of 200 kDa which does not show DNA ligase activity but that could be a preprotein.
authors.affiliation: Laboratoire de Biologie et Génétique du Développement URA CNRS 256, Université de Rennes I, France.
authors.forename: C
authors.initials: C
authors.lastname: Prigent
authors.forename: S
authors.initials: S
authors.lastname: Aoufouchi
authors.forename: M
authors.initials: M
authors.lastname: Philippe
mesh_terms: D000249:Adenosine Monophosphate / Q000378:metabolism; D011088:DNA Ligases / Q000032:analysis*; D006801:Humans; D008099:Liver / Q000201:enzymology; D008214:Lymphocytes / Q000378:metabolism*; D008970:Molecular Weight; D010449:Peptide Mapping; D011118:Polynucleotide Ligases / Q000032:analysis*; D011506:Proteins / Q000378:metabolism*; D013950:Thymus Gland / Q000378:metabolism*
chemical_list: D011506:Proteins; D000249:Adenosine Monophosphate; D011088:DNA Ligases; D011118:Polynucleotide Ligases