title: Reactivity of mast-cell-bound IgE idiotypes with anti-idiotypic antibody: mediator release or inhibition of antigen-induced mediator release?
abstract: When anti-idiotypic antibodies specific for idiotopes on IgE antibodies react with mast-cell-bound IgE antibody, the reaction may be expected to lead either to mediator release or to inhibition of allergen-induced release. This study was carried out to determine which would occur. Anti-idiotype antiserum (anti-Ids) was raised in syngeneic mice by immunisation with affinity-purified DNP-specific mouse monoclonal IgE. Sera from these immunised mice and from a similarly immunised rabbit inhibited the binding of the monoclonal IgE to radio-iodinated dinitrophenylated (DNP) ovalbumin, whereas a high concentration of affinity-purified rabbit antimouse epsilon-chain-specific antibody was unable to inhibit even though it was shown to bind to the monoclonal IgE. The mouse anti-Ids did not give a positive passive cutaneous anaphylactic reaction in rats sensitised with high-titre grass-pollen-specific IgE-containing antiserum, whereas it gave a positive result in rats sensitised with the DNP-specific monoclonal IgE. Rabbit antimonoclonal antiserum and rabbit anti-epsilon-chain antibody both gave positive results in these two assays. The results indicated that anti-idiotypic antibodies specific for idiotopes on IgE antibody can react and release mediators from IgE-sensitised mast cells. The significance of this in view of the increased levels of anti-idiotypes that can occur during immunotherapy is discussed.
authors.affiliation: Institutionen för Klinisk Immunologi, Karolinska Sjukhuset, Stockholm, Sweden.
authors.forename: A W
authors.initials: AW
authors.lastname: Wheeler
authors.forename: M J
authors.initials: MJ
authors.lastname: Deards
authors.forename: B E
authors.initials: BE
authors.lastname: Hickman
authors.forename: V M
authors.initials: VM
authors.lastname: Spackman
authors.forename: S G
authors.initials: SG
authors.lastname: Johansson
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D004136:Dinitrobenzenes / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology*; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D008407:Mast Cells / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010323:Passive Cutaneous Anaphylaxis; D011817:Rabbits
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D004136:Dinitrobenzenes; D007130:Immunoglobulin Idiotypes; D007073:Immunoglobulin E