Oxygen transport by a modified haemoglobin solution (PPSFH) in a dog model of acute anaemia following hypovolaemia.
Polymerised pyridoxylated stroma free haemoglobin (PPSFH) is a potential red cell substitute. We compared PPSFH to Succinylated Gelatin (SG) and autologous fresh blood (AFB) in a dog model. Three groups of dogs were subjected to a period of fluctuating hypovolaemia. Eight received AFB and had a final haematocrit (Hct) of 54% and total active haemoglobin level (WBod Hb) of 16.5 g/dl. Eight received SG, had a final Hct of 20% and WBod Hb of 5.6 g/dl. Eight received PPSFH, had a final Hct of 20% and a WBod Hb of 10.4 g/dl. Those dogs receiving AFB responded to the hypovolaemic stress by raising oxygen consumption by 27%, whereas in both those receiving SG and PPSFH, oxygen consumption fell by 15% (p = 0.007). In the PPSFH group the PPSFH carried 43% of oxygen consumed. Thus, although PPSFH provided a higher circulating haemoglobin level than SG and carried a high proportion of oxygen consumed, it did not confer any advantage in terms of bulk oxygen transport.
Gilroy
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D
Department of Surgery, Queen's University of Belfast, Ireland.
Odling-Smee
W
W
D000740:Anemia / Q000097:blood / Q000209:etiology / Q000628:therapy*; D000818:Animals; D001804:Blood Transfusion, Autologous; D004195:Disease Models, Animal; D004285:Dogs; D005780:Gelatin / Q000494:pharmacology / Q000627:therapeutic use*; D006400:Hematocrit; D006454:Hemoglobins / Q000032:analysis / Q000494:pharmacology / Q000627:therapeutic use*; D006470:Hemorrhage / Q000150:complications*; D010101:Oxygen Consumption / Q000187:drug effects*; D010952:Plasma Substitutes / Q000494:pharmacology / Q000627:therapeutic use*; D013386:Succinates / Q000494:pharmacology / Q000627:therapeutic use*
D006454:Hemoglobins; D010952:Plasma Substitutes; D013386:Succinates; C016484:hemoglobin, stroma free; C060085:succinylated gelatin; D005780:Gelatin