Separation by carrier-free electrophoresis of subpopulations of bovine alveolar macrophages.
Carrier-free electrophoresis was used to separate subpopulations of bovine alveolar macrophages according to their electrical surface charges. The electrophoretic mobility of the macrophages changed significantly as a function of the time of cultivation in vitro. After 20 h, three subgroups were identified which differed in their specific contents of lysosomal hydrolases, alpha-naphthyl acetate esterase, and alkaline phosphodiesterase. By comparing the findings with results from the literature it was concluded that the subgroups correspond to different stages of maturation and that the fraction moving fastest may be the most mature fraction. The results were not influenced by phagocytosis and the presence or absence of Ca2+.
Seidel
A
A
Kernforschungszentrum Karlsruhe, Institut für Genetik und Toxikologie, F.R.G.
Hammer
G
G
Polzer
G
G
D000818:Animals; D002417:Cattle; D002469:Cell Separation / Q000379:methods / Q000662:veterinary*; D004553:Electric Conductivity; D004586:Electrophoresis; D005260:Female; D008264:Macrophages / Q000201:enzymology; D008297:Male; D011650:Pulmonary Alveoli / Q000166:cytology*; D013499:Surface Properties