title: Isolation and characterization of simian immunodeficiency viruses from two subspecies of African green monkeys.
abstract: Cercopithecus aethiops (African Green monkey), a nonhuman primate species distributed throughout subsaharan Africa, has been shown to have high seroprevalence rates of antibodies to simian immunodeficiency virus (SIV), and therefore, has been proposed as a natural reservoir for immunodeficiency viruses. Our laboratories have isolated SIV-like viruses from two East African subspecies of C. aethiops designated grivet and vervet monkeys. Analysis of the structural proteins based on the molecular weights and immunologic cross-reactivity to the prototypic SIV(MAC), HIV-1, and HIV-2 isolates suggests that these viruses are distinctly different. Heterogeneity was observed in the molecular weights of the gag, pol, and env gene products between SIV isolates from vervets [SIV(AGM(VER))] and grivets [SIV(AGM(GRI))]. Phenotypically, SIV(AGM(VER)) isolates were distinguishable from SIV(AGM(GRI)) isolates by the apparent size difference of the major core antigen p24. All SIV(AGM(GRI)) and SIV(AGM(VER)) isolates were found to encode a transmembrane protein of approximately 40 kD (gp40) in contrast to gp32 of SIV(MAC). Furthermore, the transmembrane protein was shown to be encoded by the entire env open reading frame, unlike gp32 of SIC(MAC) or gp36 of SIV(AGM(TYO-1)). These data indicate that viruses from C. aethiops share common features with SIV(MAC) and HIV-1, but represent diverse SIV-like viruses which may vary according to subspecies and geographic location.
authors.affiliation: Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, TX 78284.
authors.forename: J S
authors.initials: JS
authors.lastname: Allan
authors.forename: P
authors.initials: P
authors.lastname: Kanda
authors.forename: R C
authors.initials: RC
authors.lastname: Kennedy
authors.forename: E K
authors.initials: EK
authors.lastname: Cobb
authors.forename: M
authors.initials: M
authors.lastname: Anthony
authors.forename: J W
authors.initials: JW
authors.lastname: Eichberg
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002521:Cercopithecus / Q000382:microbiology*; D002522:Chlorocebus aethiops / Q000382:microbiology*; D003429:Cross Reactions; D015497:HIV-1 / Q000032:analysis / Q000276:immunology; D015498:HIV-2 / Q000032:analysis / Q000276:immunology; D008969:Molecular Sequence Data; D015302:Simian Immunodeficiency Virus / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D014764:Viral Proteins / Q000032:analysis
chemical_list: D014764:Viral Proteins