title: Presence of interleukin-2 in urine of superficial bladder cancer patients after intravesical treatment with bacillus Calmette-Guérin.
abstract: Urine samples were obtained from patients with superficial bladder cancer after immunotherapy with bacillus Calmette-Guérin (BCG). The patients were repeatedly (once a week for 6 consecutive weeks) treated with intravesical administration of approximately 5 X 10(8) culturable particles of BCG. Some patients received more than six BCG instillations. The urine samples were investigated for the presence of interleukin-2 (IL-2) in an in vitro bioassay using a murine cytotoxic T cell line (CTTL-16) that shows IL-2-dependent growth. Preliminary experiments indicated the presence of inhibitory factors in the urine. This inhibitory activity was abolished after 24 h dialysis. In a neutralization assay with both polyvalent and monoclonal anti-(human IL-2) antibody it was demonstrated that there was indeed IL-2 in the urine samples. In 8 of 11 patients the presence of IL-2 in the urine was demonstrated. The IL-2 production was directly related to the BCG administration as samples obtained just before the BCG instillation were always negative. In IL-2-positive samples a maximum level of IL-2 was observed between 2 h and 6 h after the BCG instillation. In urine samples obtained 24 h after the BCG IL-2 was not detected. In most patients the urine became positive after the third or fourth PCG instillation.
authors.affiliation: Laboratory for Pathology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
authors.forename: W H
authors.initials: WH
authors.lastname: De Jong
authors.forename: E C
authors.initials: EC
authors.lastname: De Boer
authors.forename: A P
authors.initials: AP
authors.lastname: Van der Meijden
authors.forename: P
authors.initials: P
authors.lastname: Vegt
authors.forename: P A
authors.initials: PA
authors.lastname: Steerenberg
authors.forename: F M
authors.initials: FM
authors.lastname: Debruyne
authors.forename: E J
authors.initials: EJ
authors.lastname: Ruitenberg
mesh_terms: D001500:BCG Vaccine / Q000627:therapeutic use*; D002295:Carcinoma, Transitional Cell / Q000628:therapy*; D006801:Humans; D007118:Immunoassay; D007376:Interleukin-2 / Q000652:urine*; D001743:Urinary Bladder / Q000276:immunology; D001749:Urinary Bladder Neoplasms / Q000628:therapy*
chemical_list: D001500:BCG Vaccine; D007376:Interleukin-2