Role of protein synthesis in the accumulation of ferritin mRNA during exposure of cells to iron.
The iron-induced biosynthesis of ferritin is regulated at the translational level via multiple mechanism. A prolonged exposure of cells to iron leads to a marked increase in ferritin mRNA levels caused by stabilization of the message. Here we show that this stabilization requires the synthesis de novo of an iron-inducible protein factor.
Mattia
E
E
E.C. Slater Institute for Biochemical Research, University of Amsterdam, The Netherlands.
den Blaauwen
J
J
van Renswoude
J
J
D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D005293:Ferritins / Q000096:biosynthesis / Q000235:genetics*; D006418:Heme / Q000494:pharmacology; D006801:Humans; D007501:Iron / Q000494:pharmacology*; D007700:Kinetics; D010142:Pactamycin / Q000494:pharmacology; D011132:Polyribosomes / Q000187:drug effects / Q000378:metabolism*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D011691:Puromycin / Q000494:pharmacology; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D014158:Transcription, Genetic / Q000187:drug effects
D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D003609:Dactinomycin; D010142:Pactamycin; D006418:Heme; D011691:Puromycin; D005293:Ferritins; D003513:Cycloheximide; D007501:Iron