title: Crystallization of a soluble form of the rat T-cell surface glycoprotein CD4 complexed with Fab from the W3/25 monoclonal antibody.
abstract: The structure of the T-lymphocyte cell surface glycoprotein CD4 is of considerable biological and medical interest. Recombinant rat CD4 expressed in soluble form in mammalian cells and complexed with W3/25 monoclonal Fab fragments formed crystals that diffract to 3.5 A and have the orthorhombic space group P2(1)2(1)2 or P2(1)2(1)2(1). The unit cell has dimensions a = 317 A, b = 161 A and c = 41.8 A and the asymmetric unit consists of two CD4:Fab complexes. These crystals are of suitable quality for X-ray diffraction analysis.
authors.affiliation: MRC Cellular Immunology Research Unit, Sir William Dunn School of Pathology, Oxford, U.K.
authors.forename: S J
authors.initials: SJ
authors.lastname: Davis
authors.forename: R L
authors.initials: RL
authors.lastname: Brady
authors.forename: A N
authors.initials: AN
authors.lastname: Barclay
authors.forename: K
authors.initials: K
authors.lastname: Harlos
authors.forename: G G
authors.initials: GG
authors.lastname: Dodson
authors.forename: A F
authors.initials: AF
authors.lastname: Williams
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D015704:CD4 Antigens / Q000235:genetics / Q000276:immunology; D003460:Crystallization; D007140:Immunoglobulin Fab Fragments; D051381:Rats; D011994:Recombinant Proteins; D012995:Solubility; D014961:X-Ray Diffraction
chemical_list: D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D007140:Immunoglobulin Fab Fragments; D011994:Recombinant Proteins