Presence of specific binding sites for [3H]sarcophytol-A in cultured human skin fibroblasts.
The presence of specific binding sites for [3H]sarcophytol-A in human skin fibroblasts was examined using biochemical and morphological methods. The displacement studies clearly revealed that high (KD = 31.0 nM) and low (KD = 6.05 microM) affinity sites were present in the intact cells. Moreover, autoradiographic studies using light microscopy revealed that the specific binding sites may exist in both the cytoplasm and the nuclei.
Nakagane
T
T
Group of Neuropharmacology, Josai University, Saitama, Japan.
Ishitani
R
R
D000970:Antineoplastic Agents / Q000378:metabolism*; D001345:Autoradiography; D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism; D055598:Chemical Phenomena; D002621:Chemistry; D003593:Cytoplasm / Q000378:metabolism; D004224:Diterpenes / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D011869:Radioligand Assay; D012867:Skin / Q000166:cytology / Q000378:metabolism*; D014316:Tritium
D000970:Antineoplastic Agents; D004224:Diterpenes; D014316:Tritium; C058761:sarcophytol A