title: High sensitivity of Chinese hamster epithelial liver cells to toxic analogues of purines.
abstract: The resistance of Chinese hamster epithelial liver cells (CHEL) and Chinese hamster fibroblasts (V79) towards toxic purine analogues has been determined. The liver cells are more sensitive than fibroblasts to 6-thioguanine (6-TG), 8-azaguanine (8-AZ) and 2,6-diaminopurine (DAP). The hypoxanthine-guanine (HGPRT) and adenine phosphoribosyl transferase (APRT) activities of extracts of CHEL cells were lower than those of corresponding extracts of V79. The level of 5'-nucleotidase was about 5-fold higher in the epithelial cells. It appears that HGPRT and APRT activities of extracts of liver epithelial cells are masked or reduced by 5'-nucleotidase activity and other inhibitors. The significance of these findings is discussed.
authors.affiliation: Institute of Mutagenesis and Differentiation, CNR, Pisa, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Simili
authors.forename: P
authors.initials: P
authors.lastname: Tartaglia
authors.forename: G
authors.initials: G
authors.lastname: Turchi
mesh_terms: D015075:2-Aminopurine / Q000031:analogs & derivatives* / Q000633:toxicity; D000225:Adenine / Q000031:analogs & derivatives*; D000228:Adenine Phosphoribosyltransferase / Q000378:metabolism*; D000818:Animals; D001375:Azaguanine / Q000633:toxicity*; D002478:Cells, Cultured; D006224:Cricetinae; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000201:enzymology; D007041:Hypoxanthine Phosphoribosyltransferase / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D009152:Mutagenicity Tests; D010430:Pentosyltransferases / Q000378:metabolism*; D013866:Thioguanine / Q000633:toxicity*; D013942:Thymine Nucleotides / Q000378:metabolism
chemical_list: D013942:Thymine Nucleotides; D015075:2-Aminopurine; C007300:2,6-diaminopurine; D010430:Pentosyltransferases; D000228:Adenine Phosphoribosyltransferase; D007041:Hypoxanthine Phosphoribosyltransferase; D013866:Thioguanine; D000225:Adenine; D001375:Azaguanine; C024157:thymidine 5'-triphosphate