Effect of antibacterial plant flavanones on the intracellular calcium compartment involved in the first cleavage of sea urchin eggs.
Two plant flavanones, (Sigmoidin A and B) having noticeable antibacterial activity, were assayed using a preparation for the study of sea urchin egg cleavage. When added after insemination, both toxins inhibit egg division with a half maximal dose of 7.5 microM for Sigmoidin A and 12 microM for Sigmoidin B. The first Ca2+ signal following fertilization was not modified by the molecules, however, the intracellular storage of calcium in isolated non-mitochondrial compartments was reduced in a dose-dependent manner by Sigmoidin A and Sigmoidin B. Both trigger a complete discharge of the sequestered calcium. In vivo the flavanones dramatically reduced the capacity of storage of non-mitochondrial intracellular calcium compartments necessary to the cyclical elevation of cytosolic free calcium during the cell cycle.
Biyiti
L
L
U 303 INSERM, BP 3, Villefranche-sur-Mer, France.
Pesando
D
D
Puiseux-Dao
S
S
Girard
J P
JP
Payan
P
P
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D005419:Flavonoids / Q000494:pharmacology*; D010063:Ovum / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D012617:Sea Urchins
D000890:Anti-Infective Agents; D005419:Flavonoids; D010936:Plant Extracts; C064222:sigmoidin B; C064221:sigmoidin A; D002118:Calcium