Plasmid profiles of six species of Campylobacter from human beings, swine, and sheep.
Twenty-four isolates representing 6 species of Campylobacter were screened for plasmids. A large plasmid with an approximate molecular weight of 38 Mdal was detected in 5 C jejuni isolates originally recovered from diarrheic human beings, in one isolate of C coli recovered from diarrheic pigs, and in 1 isolate of C sputorum ssp mucosalis and 2 isolates of C hyointestinalis recovered from pigs with proliferative enteritis. One isolate of C coli and 1 isolate of C hyointestinalis contained an additional smaller plasmid with an approximate molecular weight of 1.6 Mdal; this plasmid was partially mapped by restriction endonuclease digestion. Fifteen Campylobacter isolates contained no detectable plasmids: 2 C coli, 2 C sputorum ssp mucosalis, 2 C fecalis, 1 C fetus ssp fetus, and 8 C hyointestinalis isolates. In summary, 37.5% of the Campylobacter isolates contained a 38-Mdal plasmid, with 8% having both 38 Mdal and 1.6-Mdal plasmids; 62.5% contained no detectable plasmids.
Boosinger
T R
TR
Department of Pathobiology, College of Veterinary Medicine, Auburn University, AL 36849.
Blevins
W T
WT
Heron
J V
JV
Sunter
J L
JL
D000818:Animals; D002167:Campylobacter / Q000235:genetics* / Q000302:isolation & purification; D003470:Culture Media; D004269:DNA, Bacterial / Q000302:isolation & purification; D006801:Humans; D008970:Molecular Weight; D010957:Plasmids / Q000235:genetics*; D012756:Sheep / Q000382:microbiology*; D013045:Species Specificity; D013552:Swine / Q000382:microbiology*
D003470:Culture Media; D004269:DNA, Bacterial