title: Determination of tauromustine and its demethylated metabolites in plasma and urine.
abstract: A sensitive, selective and precise high-performance liquid chromatographic method for simultaneous determination of tauromustine and its demethylated metabolites in plasma and urine has been developed. It is based on solid-phase extraction on C18 sorbent and separation on a semipolar column. The analytical procedure is described in detail. The method has been validated with respect to linearity, recovery, selectivity, precision and detection limit. The stability of the determined substances in various media has also been studied.
authors.affiliation: Pharmacia LEO Therapeutics AB, Helsingborg, Sweden.
authors.forename: J
authors.initials: J
authors.lastname: Polacek
authors.forename: B
authors.initials: B
authors.lastname: Gustafsson
authors.forename: S
authors.initials: S
authors.lastname: Brandin
authors.forename: A
authors.initials: A
authors.lastname: Ottersgård-Brorsson
mesh_terms: D000970:Antineoplastic Agents / Q000097:blood* / Q000493:pharmacokinetics / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D009607:Nitrosourea Compounds / Q000097:blood* / Q000493:pharmacokinetics / Q000652:urine; D013654:Taurine / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics / Q000652:urine
chemical_list: D000970:Antineoplastic Agents; D009607:Nitrosourea Compounds; D013654:Taurine; C052991:1-(2-chloroethyl)-3-(2-(dimethylaminosulfonyl)ethyl)-1-nitrosourea