title: Characterization of phosphate uptake in primary cultures of rat hepatocytes.
abstract: Phosphate uptake across the plasma membrane of rat hepatocytes was characterized in primary cultures of rat hepatocytes. Phosphate uptake by cultured rat hepatocytes consisted of a Na(+)-dependent and Na(+)-independent component. A major portion of the uptake was Na(+)-dependent, which was saturable and exhibited Michaelis-Menten kinetics. Kinetic analysis showed that the apparent Km value for phosphate at 137 mM Na+ was 0.25 mM and Vmax was 13.3 pmol/microgram protein for 30 min. Hill coefficient was 1.76. This study is the first to demonstrate the presence of a phosphate carrier on the plasma membrane of hepatocytes.
authors.affiliation: Department of Biochemistry, Michigan State University, East Lansing 48824.
authors.forename: H
authors.initials: H
authors.lastname: Arai
authors.forename: W W
authors.initials: WW
authors.lastname: Wells
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000502:physiology; D002478:Cells, Cultured; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D010710:Phosphates / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000378:metabolism
chemical_list: D010710:Phosphates; D012964:Sodium