Serum haemolytic complement activity and C3 levels in bovine trypanosomosis under natural conditions of challenge--early indications of individual susceptibility to disease.
Twenty-five Baoule (Bos taurus) and 12 Zebu (Bos indicus) cattle, which were part of an experiment aimed at characterizing cattle for resistance to trypanosomosis under natural challenge in Burkina Faso, were monitored for complement levels. Total haemolytic activity of the alternative complement pathway and C3 in sera taken weekly were estimated. The results were analysed in relation to the course of the disease, parasitological data, packed red cell volume (PCV) and body weight. All the animals became infected with Trypanosoma vivax and/or T. congolense. The Zebu had to be treated with Berenil (Diminazene aceturate, Hoechst, W. Germany) after a mean period of 5 weeks of infection, whereas 7 of the 25 Baoule remained in good condition throughout the experiment. The remaining 18 Baoule required treatment after a variable period of infection. There was a decrease in haemolytic complement activity (HC') as well as in C3 levels, which coincided with the first detection of parasites in the blood. The titres in the Zebu fell to 10-20% of pre-infection level within 2-3 weeks and they showed no tendency towards regaining normal levels. The drop in complement in the Baoule was less pronounced and was in most cases followed by an increase approaching normal values. In these animals, the complement level in early infection was found to depend on the intensity of parasite load and on the control potential of each individual. There was a significant correlation between minimum complement activity (min. HC'), minimum C3 (min C3) and minimum PCV (min.PCV) in early infection. These three parameters correlated with individual resistance and might, therefore, be useful criteria for the identification of the most resistant individuals within a trypanotolerant breed.
Authié
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C.R.T.A.B.P. 454-Bobo-Dioulasso, Burkina Faso, West Africa.
Pobel
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D000818:Animals; D001835:Body Weight; D002417:Cattle; D003176:Complement C3 / Q000096:biosynthesis*; D015941:Complement Hemolytic Activity Assay; D003170:Complement Pathway, Alternative; D003165:Complement System Proteins / Q000096:biosynthesis*; D004198:Disease Susceptibility; D005260:Female; D006400:Hematocrit / Q000662:veterinary; D007700:Kinetics; D008297:Male; D014348:Trypanosoma congolense / Q000276:immunology; D014353:Trypanosomiasis, African / Q000097:blood / Q000276:immunology / Q000662:veterinary; D014354:Trypanosomiasis, Bovine / Q000097:blood / Q000276:immunology*
D003176:Complement C3; D003165:Complement System Proteins