title: [Monoclonal antibodies IPO-4 recognizing antigen-activated human T and B lymphocytes].
abstract: Spleen cells from BALB/c mice previously immunized with B-lymphoblastoid cell line RPMI-1788 were fused with P3-X-63-Ag8.653 myeloma cells. Monoclonal antibodies (Ab) IPO-4 were screened on 18 cell lines by the indirect immuno-fluorescence method. Cryostat sections of tissues were stained according to the PAP technique. The Mab IPO-4 were tested for reactivity with blood cells of 17 healthy persons and 102 patients with chronic lymphocytic leukemia, acute lymphoblastic and myeloblastic leukemias, hairy cell leukemia, multiple myeloma, non-Hodgkin's lymphomas and Hodgkin's disease and mitogen stimulated lymphocytes. MAb IPO-4 were found to be directed against antigen expressed on activated T and B cells.
authors.forename: S P
authors.initials: SP
authors.lastname: Sidorenko
authors.forename: A G
authors.initials: AG
authors.lastname: Berdova
authors.forename: E P
authors.initials: EP
authors.lastname: Vetrova
authors.forename: L N
authors.initials: LN
authors.lastname: Shlapatskaia
authors.forename: O V
authors.initials: OV
authors.lastname: Iurchenko
authors.forename: A I
authors.initials: AI
authors.lastname: Evsev'eva
authors.forename: D F
authors.initials: DF
authors.lastname: Gluzman
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000944:Antigens, Differentiation, B-Lymphocyte / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D014408:Biomarkers, Tumor; D002460:Cell Line; D006801:Humans; D007938:Leukemia / Q000175:diagnosis / Q000276:immunology; D008213:Lymphocyte Activation; D008223:Lymphoma / Q000175:diagnosis / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009101:Multiple Myeloma / Q000175:diagnosis / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000944:Antigens, Differentiation, B-Lymphocyte; D000945:Antigens, Differentiation, T-Lymphocyte; D014408:Biomarkers, Tumor
vernacular_title: Monoklonal'nye antitela IPO-4, raspoznaiushchie antigen-aktirovannye T- i B-limfotsity cheloveka.