[Evaluation of catalytic activity of multiple forms of cytochrome P-450 in liver microsomes of Wistar rats by androstenedione metabolism].
The synthesis of androstenedione (AD) metabolites in liver microsomes of control and phenobarbital (PB)-induced male and female Wistar rats was studied. In liver microsomes of non-induced male rats the predominant formation of 16 alpha-hydroxyandrostenedione was observed, whereas in females the predominant form was the 7 alpha-metabolite. Induction of animals of both sexes with PB was accompanied by an increase in the 16 beta-hydroxylase activity. The dynamics of synthesis of other AD metabolites specifically produced by such cytochrome P-450 forms as P-450a, P-450h and P-450p was investigated. The specificity of metabolism of androstenedione by multiple forms of cytochrome P-450 is postulated.
Khatsenko
O G
OG
Guliaeva
L F
LF
Mishin
V M
VM
Liakhovich
V V
VV
D000735:Androstenedione / Q000378:metabolism*; D000818:Animals; D002384:Catalysis; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000378:metabolism*; D004790:Enzyme Induction; D005260:Female; D006900:Hydroxylation; D007527:Isoenzymes / Q000096:biosynthesis / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D007527:Isoenzymes; D000735:Androstenedione; D003577:Cytochrome P-450 Enzyme System; D010634:Phenobarbital
Otsenka kataliticheskoĭ aktivnosti mnozhestvennykh form tsitokhroma P-450 v mikrosomakh pecheni krys linii Vistar po metabolizmu androstendiona.