Collagen shield delivery of amphotericin B.
By using a high-pressure liquid chromatography assay, we investigated the ability of collagen shield therapeutic contact lenses to release amphotericin B and deliver it to the anterior segment of rabbit eyes. In vitro studies showed that presoaked collagen shields released most of the amphotericin B within the first hour of elution. We compared the corneal and aqueous humor amphotericin B levels produced by collagen shields soaked in amphotericin B and frequent-drop therapy at four time points over a six-hour period. The collagen shields soaked in amphotericin B produced corneal levels that were higher than those produced by frequent-drop therapy at one hour, equivalent to drop therapy at two and three hours, and lower than drop therapy at six hours. There were no differences in amphotericin B levels in aqueous humor at any time point between rabbits treated with collagen shield delivery and rabbits treated with frequent-drop delivery. The results of this study suggest that amphotericin B delivery to the cornea by collagen shields is comparable to frequent-drop delivery but has the potential benefit of added convenience and compliance.
Schwartz
S D
SD
Jules Stein Eye Institute, UCLA Center for Health Sciences 90024-7000.
Harrison
S A
SA
Engstrom
R E
RE
Bawdon
R E
RE
Lee
D A
DA
Mondino
B J
BJ
D000287:Administration, Topical; D000666:Amphotericin B / Q000008:administration & dosage* / Q000493:pharmacokinetics; D000818:Animals; D000867:Anterior Chamber / Q000378:metabolism; D001082:Aqueous Humor / Q000378:metabolism; D001458:Bandages; D001684:Biological Dressings; D003094:Collagen; D003261:Contact Lenses; D003315:Cornea / Q000378:metabolism; D005260:Female; D011817:Rabbits
D000666:Amphotericin B; D003094:Collagen