title: Fibroblast behavior at aqueous interfaces with perfluorocarbon, silicone, and fluorosilicone liquids.
abstract: Perfluorocarbon, silicone, and fluorosilicone liquids with potential for use as vitreous substitutes in the management of complex retinal detachment were evaluated for surface reactivity by assessing the behavior of anchorage-dependent fibroblasts plated at the phase boundary between these compounds and culture medium. Low-viscosity perfluorcarbons were alumina-treated to remove polar impurities. On perfluorodecalin, perfluorodimethylcyclohexane, perfluorotrimethylcyclohexane, perfluoroethylcyclohexane, perfluorooctane, perfluoroperhydrophenanthrene, perfluoromethyladamantane, perfluorodimethyladamantane, the highly viscous perfluoropolyether liquids Krytox TLF7067 and 6354, and dimethylsiloxane liquids of a variety of viscosities, most cells did not attach; the few that did attach exhibited minimal spreading behavior and did not achieve the flattened spindle-shape morphology which is a prerequisite to normal proliferative activity. Conversely, on perfluoromethyldecaline, perfluorofluorene, perfluorotributylamine, the perfluoropolyether K-6 hexamer, trifluoropropylmethylsiloxane (fluorosilicone), and diphenyldimethylsiloxane, some cells became fusiform-shaped and exhibited proliferation, the extent of which varied with the compound. The association of alumina treatment of perfluorocarbon liquids with a reduction in cell growth was indicative of a relationship between the presence of residual hydrogen-containing impurities and the capacity for cellular attachment and growth. This correlation was demonstrated also in experiments in which cell attachment and growth was facilitated by the addition of hydrogen-rich monohydroperfluorooctane to alumina-treated perfluorooctane. In conclusion, evidence for the presence of surface active impurities in liquid vitreous substitute materials can be obtained by observing the behavior of attachment-dependent cells plated at the boundary between these compounds and culture medium.
authors.affiliation: Department of Ophthalmology, New York Hospital, Cornell University Medical Center, NY 10021.
authors.forename: J R
authors.initials: JR
authors.lastname: Sparrow
authors.forename: R
authors.initials: R
authors.lastname: Ortiz
authors.forename: P R
authors.initials: PR
authors.lastname: MacLeish
authors.forename: S
authors.initials: S
authors.lastname: Chang
mesh_terms: D000537:Aluminum Oxide / Q000494:pharmacology; D000818:Animals; D001082:Aqueous Humor; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D055598:Chemical Phenomena; D002621:Chemistry; D004129:Dimethylpolysiloxanes / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology*; D005466:Fluorocarbons / Q000494:pharmacology*; D012828:Silicones / Q000494:pharmacology*
chemical_list: D004129:Dimethylpolysiloxanes; D005466:Fluorocarbons; D012828:Silicones; C049525:polymethyl-3,3,3-trifluoropropylsiloxane; D000537:Aluminum Oxide