A polymorphic microsatellite in the tumor necrosis factor alpha promoter identifies an allele unique to the NZW mouse strain.
We have amplified a (CA)n:(GT)n microsatellite from the TNF promoters of a panel of mouse strains using the polymerase chain reaction. The length of the microsatellites was polymorphic, with eight alleles observed among 15 inbred strains bearing seven distinct H-2 haplotypes, and four outbred strains. In B10 congenic strains, the TNF allele detected by microsatellite polymorphism segregated with the MHC, and in recombinant haplotypes (NOD, NZW), it segregated with H-2D. The TNF allele found in the NZW strain (H-2z) was distinct from those of all other haplotypes, consistent with the hypothesis that this strain may carry a genetic defect in TNF production.
Jongeneel
C V
CV
Ludwig Institute for Cancer Research, Epalinges, Switzerland.
Acha-Orbea
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H
Blankenstein
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T
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004276:DNA, Satellite / Q000235:genetics*; D005819:Genetic Markers; D006239:Haplotypes; D008099:Liver / Q000166:cytology; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013045:Species Specificity; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D004276:DNA, Satellite; D005819:Genetic Markers; D014409:Tumor Necrosis Factor-alpha; D004247:DNA