Rapid analysis of non-esterified fatty acids as methyl esters from different biological specimens by gas chromatography after one-step esterification.
A rapid gas chromatographic method for the determination of medium-chain and long-chain free fatty acids (C14:0 to C24:0 fatty acids) from different biological specimens is presented. After a rapid one-step transesterification method in methanol-acetyl chloride (50:1, v/v), fatty acid methyl esters were extracted into n-hexane and analysed on a 15-m Durabond-Wax column within a 12-min chromatographic run. The detection limit is 500 pg per injection.
Welz
W
W
Institute of Pediatrics, Department of Mass Spectrometry, Graz, Austria.
Sattler
W
W
Leis
H J
HJ
Malle
E
E
D000818:Animals; D001322:Autoanalysis; D002849:Chromatography, Gas; D004951:Esterification; D005230:Fatty Acids, Nonesterified / Q000032:analysis* / Q000097:blood; D006801:Humans; D008745:Methylation; D008862:Microsomes, Liver / Q000032:analysis; D051381:Rats; D012997:Solvents
D005230:Fatty Acids, Nonesterified; D012997:Solvents