Plasma separator for encapsulating fluorescent probe in erythrocytes.
Using a plasma separator, it is possible to wash large volumes of whole blood free of plasma proteins in short periods. The novelty, however, is that the same apparatus can be used to reversibly hemolyze and reseal the red blood cells (RBCs) using hypotonic and hypertonic dialysates, respectively. This technique was assessed using the fluorescent exogenous agent uranin (fluorescein sodium). Results indicate that the encapsulation of the RBCs can be completed in under 2 h. The encapsulation efficiency of the RBCs was approximately 80% with a RBC recovery rate of 75%.
el-Kalay
M A
MA
Applied Immune Sciences, Inc., Menlo Park, California 94025-1109.
Abdel-Kader
M H
MH
Hamzah
R Y
RY
al-Shirbini
S A
SA
D002469:Cell Separation / Q000295:instrumentation*; D004337:Drug Carriers; D004910:Erythrocyte Membrane; D004912:Erythrocytes; D019793:Fluorescein; D005452:Fluoresceins; D006801:Humans
D004337:Drug Carriers; D005452:Fluoresceins; D019793:Fluorescein