Effect of chitin heparinoids on the activation of peritoneal macrophages and on the production of monokines in mice.
Sulfonated derivatives of chitin which showed anticoagulant activity (chitin heparinoids) were studied with regard to the activation of mouse peritoneal macrophages and the production of monokines. In comparison with 70% deacetylated chitin (DAC-70), which was the most adjuvant-active derivative of chitin, all chitin heparinoids were less effective for the augmentation of cytolytic activity of peritoneal macrophages. The number of macrophages was hardly increased or decreased by intraperitoneal injection of chitin heparinoids, and the activity of circulating colony-stimulating factor was not changed by their treatment. Only N-sulfonated DAC-70 stimulated the production of interleukin-1 by thioglycolate-induced peritoneal macrophages in vitro. However, its effect was weaker than that of DAC-70. Chitin heparinoids showed no or weak mitogenic activity on normal mouse spleen cells.
Nishimura
K
K
Institute of Immunological Science, Faculty of Science, Hokkaido University, Sapporo, Japan.
Nishimura
S
S
Nishi
N
N
Tokura
S
S
Azuma
I
I
D000276:Adjuvants, Immunologic; D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D002478:Cells, Cultured; D002686:Chitin / Q000031:analogs & derivatives* / Q000494:pharmacology; D003115:Colony-Stimulating Factors / Q000096:biosynthesis; D005260:Female; D006493:Heparin / Q000494:pharmacology; D007375:Interleukin-1 / Q000096:biosynthesis; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008934:Mitogens; D015846:Monokines / Q000096:biosynthesis*; D013154:Spleen / Q000166:cytology
D000276:Adjuvants, Immunologic; D000925:Anticoagulants; D003115:Colony-Stimulating Factors; D007375:Interleukin-1; D008934:Mitogens; D015846:Monokines; D002686:Chitin; D006493:Heparin