[The quantitative determination of Bacillus thuringiensis beta-exotoxin in insecticidal biopreparations].
A method of quantitative determination of beta-exotoxin content in liquid and dry bioformulations has been developed. The method includes a thin-layer chromatography to isolate beta-exotoxin from accompanying nucleotides, the further desorption of a single beta-exotoxin spot by water and to carry out spectrophotometry at 259 and 330 nm. beta-exotoxin content in industrial formulations bitoxibacillin and turingin I has been determined. The results obtained correspond to the NMR 1H spectroscopy data within the experimental errors. The relative error is 1-2%. The method sensitivity of 0.05 mg/ml. beta-exotoxin content at biotechnological stages of bitoxibacillin production has been determined.
Efimtsev
E I
EI
Burov
G P
GP
Solomin
A A
AA
D001413:Bacillus thuringiensis; D002855:Chromatography, Thin Layer; D005098:Exotoxins / Q000032:analysis*; D007306:Insecticides / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D009930:Organic Chemicals; D013056:Spectrophotometry, Ultraviolet
D005098:Exotoxins; D007306:Insecticides; D009930:Organic Chemicals; C021633:Bitoxibacillin
Kolichestvennoe opredelenie beta-ékzotoksina Bacillus thuringiensis v insektitsidnykh biopreparatakh.