Measurement of human embryo-derived platelet-activating factor (PAF) using a quantitative bioassay of platelet aggregation.
A quantitative bioassay was used to measure the concentration of platelet activating factor (PAF) in medium in which human embryos produced by IVF had been cultured and in various other biological fluids. Following extraction and partial purification, 121 of 228 (53%) media samples in which single human embryos were cultured for 24 h had PAF levels greater than found in corresponding control media. This was assigned as embryo-derived PAF and the corresponding embryos termed 'PAF-positive'. Medium from those PAF-positive embryos transferred to patients who achieved an ongoing pregnancy had a mean PAF concentration of 295 +/- 107 nM (mean +/- SEM, n = 55), which was significantly greater (P less than 0.03) than media of PAF-positive embryos transferred to patients who failed to become pregnant (75 +/- 27 nM, n = 66, t-test). The embryos with the faster cleavage rates tended to secrete more PAF (P less than 0.01). Although a greater proportion of culture media derived from embryos transferred to patients who achieved a pregnancy were PAF-positive (66 out of 121, 54.5%) compared with those transferred to patients who failed to achieve a pregnancy (55 out of 121, 45.4%), this was not significant (P greater than 0.05). It was observed that 13% of women who achieved a pregnancy had embryos transferred which did not produce significant amounts of PAF in vitro. This occurred in 26% of women not achieving pregnancy.(ABSTRACT TRUNCATED AT 250 WORDS)
Collier
M
M
Human Reproduction Unit, Royal North Shore Hospital, Sydney, St Leonards, Australia.
O'Neill
C
C
Ammit
A J
AJ
Saunders
D M
DM
D001681:Biological Assay; D002478:Cells, Cultured; D003470:Culture Media / Q000032:analysis; D006801:Humans; D010972:Platelet Activating Factor / Q000032:analysis*; D010974:Platelet Aggregation
D003470:Culture Media; D010972:Platelet Activating Factor