title: Immunogenicity and allergenicity of Culicoides imicola (Diptera: Ceratopogonidae) extracts.
abstract: Summer seasonal recurrent dermatitis (SSRD) or "sweet itch" is a seasonally occurring allergic dermatitis of horses provoked by biting midges. The allergic skin reactions have been attributed to allergens present in various Culicoides species. C. imicola is the suspected etiological agent of SSRD in Israel. Whole body extracts of this midge induced hypersensitivity reactions upon injection into susceptible horses and in this study attempts were made to define components of C. imicola which have immunogenic and allergenic properties. Immunogenic potency was evaluated by raising antisera to whole body extracts of C. imicola in rabbits and examining their reactivity towards fractionated extracts. Allergenic potency was examined by reacting fractionated extracts with horse sera. Humoral reactivity of susceptible and non susceptible horses was assayed by specific IgE and IgG ELISAs. Although there are many antigenic components in whole body extracts of C.imicola capable of eliciting an immune response, no conclusive evidence was obtained indicating that allergic reactivity was associated with increased IgE levels of defined specificity.
authors.affiliation: Kimron Veterinary Institute, Beit Dagan, Israel.
authors.forename: H
authors.initials: H
authors.lastname: Ungar-Waron
authors.forename: Y
authors.initials: Y
authors.lastname: Braverman
authors.forename: A
authors.initials: A
authors.lastname: Gluckman
authors.forename: Z
authors.initials: Z
authors.lastname: Trainin
mesh_terms: D000485:Allergens / Q000276:immunology*; D000818:Animals; D015153:Blotting, Western; D002519:Ceratopogonidae / Q000276:immunology*; D002850:Chromatography, Gel; D004478:Ectoparasitic Infestations / Q000276:immunology / Q000662:veterinary*; D006734:Horse Diseases / Q000276:immunology*; D006736:Horses / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology
chemical_list: D000485:Allergens; D007106:Immune Sera