One pot solution synthesis of cyclic oligodeoxyribonucleotides.
Several cyclic oligodeoxynucleotides with different base composition and size have been prepared from 5',3'-unprotected linear precursors, using a bifunctional phosphorylating reagent. The final deprotected oligomers have been characterized by 1H- and 31P-NMR. The present procedure is particularly useful for millimolar scale syntheses.
Capobianco
M L
ML
ICoCEA-CNR, Bologna, Italy.
Carcuro
A
A
Tondelli
L
L
Garbesi
A
A
Bonora
G M
GM
D001483:Base Sequence; D009682:Magnetic Resonance Spectroscopy; D008722:Methods; D015394:Molecular Structure; D009712:Nucleotides, Cyclic / Q000138:chemical synthesis* / Q000302:isolation & purification; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000302:isolation & purification; D010766:Phosphorylation
D009712:Nucleotides, Cyclic; D009838:Oligodeoxyribonucleotides