Production of alpha 1-proteinase inhibitor (human).
A method for large scale isolation of alpha 1-proteinase inhibitor (alpha 1-PI) is described. This method employs waste Cohn Fraction IV-1 as the starting material and involves fractional precipitation with polyethylene glycol followed by ion exchange chromatography on diethylaminoethanol (DEAE)-Sepharose. The process also incorporates a ten hour, at 60 degrees C, heat-treatment step to reduce or eliminate the risk of transmission of viral disease. The final product, having a purity of approximately 60%, is freeze-dried. This preparation behaves almost identically to the alpha 1-PI in plasma and is suitable for replacement therapy in hereditary emphysema.
Hein
R H
RH
Plasma Manufacturing Technology, Cutter Biological, Miles Inc., Berkeley, California.
Van Beveren
S M
SM
Shearer
M A
MA
Coan
M H
MH
Brockway
W J
WJ
D002851:Chromatography, High Pressure Liquid; D004588:Electrophoresis, Cellulose Acetate; D006801:Humans; D008722:Methods; D000515:alpha 1-Antitrypsin / Q000032:analysis / Q000302:isolation & purification*
D000515:alpha 1-Antitrypsin