Purification and characterization of two acidic phospholipase A2 enzymes from king cobra (Ophiophagus hannah) snake venom.
1. The two major phospholipase A2 enzymes (OHPLA-DE1 and OHPLA-DE2) of king cobra (Ophiophagus hannah) venom have been purified to electrophoretic homogeneity. 2. The isoelectric points of OHPLA-DE1 and OHPLA-DE2 were 3.81 and 3.89, respectively and the Mws were 14,000 and 15,000, respectively, as estimated by Sephadex G-75 gel filtration chromatography; and 14,000 as estimated by SDS-PAGE. 3. The enzymes were not lethal to mice at a dosage of 10 micrograms/g body wt by i.v. route. Both phospholipase A2 enzymes, however, exhibited moderate edema-inducing and anti-coagulant activities. 4. Bromophenacylation of the enzymes reduced the enzymatic activity drastically but did not affect the edema-inducing activity of the enzymes.
Tan
N H
NH
Department of Biochemistry, University of Malaya, Kuala Lumpur, Malaysia.
Saifuddin
M N
MN
D000098:Acetophenones; D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D004487:Edema / Q000139:chemically induced; D004546:Elapid Venoms / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D007526:Isoelectric Point; D051379:Mice; D008970:Molecular Weight; D010740:Phospholipases / Q000302:isolation & purification*; D010741:Phospholipases A / Q000302:isolation & purification* / Q000494:pharmacology / Q000633:toxicity; D054467:Phospholipases A2
D000098:Acetophenones; D004546:Elapid Venoms; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; C006821:4-bromophenacyl bromide