Separation of oligomannose-type sugar chains having one to five mannose residues by high-performance liquid chromatography as their pyridylamino derivatives.
Ten oligomannose-type sugar chains (ManGlcNAc2-Man5GlcNAc2) were prepared from various glycoproteins and fluorescence labeled with 2-aminopyridine. The fluorescent pyridylamino (PA)-sugar chains were first separated into five fractions according to their molecular sizes by HPLC on a TSK gel Amide-80 column. Each fraction was then separated into the component PA-sugar chains by reversed-phase HPLC on a Capcell Pak C18 column according to their chemical structures. The method is useful for studying the substrate specificities of alpha-mannosidases with Man5GlcNAc2-PA as a substrate.
Oku
H
H
Department of Chemistry, Osaka University College of Science, Japan.
Hase
S
S
Ikenaka
T
T
D000631:Aminopyridines / Q000378:metabolism; D000818:Animals; D002240:Carbohydrate Sequence; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D007887:Fabaceae; D008358:Mannose / Q000302:isolation & purification*; D008361:Mannosidases / Q000378:metabolism; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000302:isolation & purification*; D010946:Plants, Medicinal; D011784:Quail; D013379:Substrate Specificity
D000631:Aminopyridines; D009844:Oligosaccharides; D008361:Mannosidases; D008358:Mannose; C032439:alpha-aminopyridine