The importance of the C-terminal amide structure of rat pancreastatin to inhibit pancreatic exocrine secretion.
A C-terminal fragment of rat pancreatatin, a 26 residue peptide amide and a fragment without a C-terminal amide were synthesized by Fmoc-based solid phase methods and their biological activities were compared. The rat C-terminal fragment inhibited pancreatic exocrine secretions produced by the intravenous injection of 2-deoxy-D-glucose (a central vagal nerve stimulation), whereas the fragment without a C-terminal amide showed no effect on pancreas. These results indicate that the C-terminal amide of this peptide is necessary to reveal its biological activity.
Miyasaka
K
K
First Laboratory of Clinical Physiology, Tokyo Metropolitan Institute of Gerontology, Japan.
Funakoshi
A
A
Kitani
K
K
Tamamura
H
H
Fujii
N
N
Funakoshi
S
S
D000818:Animals; D053379:Chromogranin A; D003847:Deoxyglucose / Q000494:pharmacology; D008297:Male; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism*; D010187:Pancreatic Hormones / Q000502:physiology*; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013329:Structure-Activity Relationship
D053379:Chromogranin A; D010187:Pancreatic Hormones; D010446:Peptide Fragments; C050836:pancreastatin; D003847:Deoxyglucose