[Early effects of sodium iodate shown by detection of the destruction of anionic sites in outer blood-retinal barrier].
In order to demonstrate the early effect of sodium iodate (NaIO3) on retinal pigment epithelium (RPE), Bruch's membrane and choriocapillaries, we examined the alteration of their anionic sites using a cationic probe, polyethyleneimine (PEI). We injected 10 microliters of 3% NaIO3 solution into the vitreous of pigmented rabbits and after thirty minutes or one hour, administered 0.5% PEI intravenously. In the eyes injected with intravitreously saline solution or without injection, PEI-positive particles (diameter 15-20 nm) were located in a row on both sides of the basement membrane of the RPE and choriocapillaries and periodically at the collagen fiber of inner and outer collagenous zone of Bruch's membrane. However no particle was observed on the cell membrane of the basal infolding of the RPE. Thirty minutes after injection of NaIO3, PEI particle positive sites were reduced at the basement membrane of the RPE. One hour after injection they reduced in number but remained at the basement membrane of choriocapillaris and collagens in Bruch's membrane. We showed the early effect of NaIO3 to the RPE by examining the alterations of the anionic sites.
Kishimoto
N
N
Dept. of Ophthalmol., Kansai Medical Univ., Moriguchi, Japan.
Ohkuma
H
H
Yamagishi
K
K
Uyama
M
M
D000818:Animals; D000838:Anions / Q000378:metabolism*; D001485:Basement Membrane / Q000187:drug effects / Q000648:ultrastructure; D001813:Blood-Retinal Barrier / Q000187:drug effects*; D007452:Iodates / Q000009:adverse effects*; D007455:Iodine / Q000009:adverse effects*; D010857:Pigment Epithelium of Eye / Q000187:drug effects* / Q000378:metabolism; D011094:Polyethyleneimine; D011817:Rabbits
D000838:Anions; D007452:Iodates; D011094:Polyethyleneimine; D007455:Iodine; C032285:sodium iodate