High sensitivity of Chinese hamster epithelial liver cells to toxic analogues of purines.
The resistance of Chinese hamster epithelial liver cells (CHEL) and Chinese hamster fibroblasts (V79) towards toxic purine analogues has been determined. The liver cells are more sensitive than fibroblasts to 6-thioguanine (6-TG), 8-azaguanine (8-AZ) and 2,6-diaminopurine (DAP). The hypoxanthine-guanine (HGPRT) and adenine phosphoribosyl transferase (APRT) activities of extracts of CHEL cells were lower than those of corresponding extracts of V79. The level of 5'-nucleotidase was about 5-fold higher in the epithelial cells. It appears that HGPRT and APRT activities of extracts of liver epithelial cells are masked or reduced by 5'-nucleotidase activity and other inhibitors. The significance of these findings is discussed.
Simili
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M
Institute of Mutagenesis and Differentiation, CNR, Pisa, Italy.
Tartaglia
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P
Turchi
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G
D015075:2-Aminopurine / Q000031:analogs & derivatives* / Q000633:toxicity; D000225:Adenine / Q000031:analogs & derivatives*; D000228:Adenine Phosphoribosyltransferase / Q000378:metabolism*; D000818:Animals; D001375:Azaguanine / Q000633:toxicity*; D002478:Cells, Cultured; D006224:Cricetinae; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000201:enzymology; D007041:Hypoxanthine Phosphoribosyltransferase / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D009152:Mutagenicity Tests; D010430:Pentosyltransferases / Q000378:metabolism*; D013866:Thioguanine / Q000633:toxicity*; D013942:Thymine Nucleotides / Q000378:metabolism
D013942:Thymine Nucleotides; D015075:2-Aminopurine; C007300:2,6-diaminopurine; D010430:Pentosyltransferases; D000228:Adenine Phosphoribosyltransferase; D007041:Hypoxanthine Phosphoribosyltransferase; D013866:Thioguanine; D000225:Adenine; D001375:Azaguanine; C024157:thymidine 5'-triphosphate