title: Simultaneous determination of plasma noradrenaline and adrenaline kinetics. Responses to nitroprusside-induced hypotension and 2-deoxyglucose-induced glucopenia in the rabbit.
abstract: Liquid chromatographic fractionation and detection of exogenous radiolabelled and endogenous catechols was used to examine simultaneously the plasma kinetics of noradrenaline and adrenaline in the conscious rabbit. Plasma clearances and release of noradrenaline and adrenaline into plasma were compared before and during nitroprusside-induced hypotension and 2-deoxyglucose-induced glucopenia, stimuli purported to differentially affect catecholamine release from sympathetic neurons and the adrenal medulla. Plasma concentrations of dihydroxyphenylglycol (DHPG) were also measured to assess presynaptic sympathetic function. Plasma clearances of adrenaline correlated with, but were significantly less than those of noradrenaline. Plasma clearances of both catecholamines showed significant decreases during nitroprusside-induced hypotension and 2-deoxyglucose-induced glucopenia. Glucopenia and hypotension increased the release into plasma of noradrenaline and adrenaline, but the adrenaline response relative to the noradrenaline response was greater during glucopenia than during hypotension. Plasma DHPG concentrations increased during glucopenia and hypotension, consistent with increased neuronal reuptake of noradrenaline and therefore a neuronal source--as opposed to an adrenal source--of most of the noradrenaline appearing in plasma during the stimuli. The increase in plasma DHPG relative to that of noradrenaline was greater after 2-deoxyglucose than after nitroprusside suggesting that the presynaptic handling of noradrenaline during glucopenia was different from that during hypotension or that the two stimuli released DHPG from regionally distinct sources.
authors.affiliation: Baker Medical Research Institute, Prahran, Victoria, Australia.
authors.forename: O S
authors.initials: OS
authors.lastname: Medvedev
authors.forename: M D
authors.initials: MD
authors.lastname: Esler
authors.forename: J A
authors.initials: JA
authors.lastname: Angus
authors.forename: H S
authors.initials: HS
authors.lastname: Cox
authors.forename: G
authors.initials: G
authors.lastname: Eisenhofer
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001794:Blood Pressure / Q000187:drug effects*; D002853:Chromatography, Liquid; D003837:Deoxy Sugars / Q000494:pharmacology*; D003847:Deoxyglucose / Q000494:pharmacology*; D004837:Epinephrine / Q000097:blood* / Q000493:pharmacokinetics; D005260:Female; D005292:Ferricyanides / Q000494:pharmacology*; D006339:Heart Rate / Q000187:drug effects; D008297:Male; D008734:Methoxyhydroxyphenylglycol / Q000031:analogs & derivatives / Q000097:blood; D009599:Nitroprusside / Q000494:pharmacology*; D009638:Norepinephrine / Q000097:blood* / Q000493:pharmacokinetics; D011817:Rabbits
chemical_list: D001786:Blood Glucose; D003837:Deoxy Sugars; D005292:Ferricyanides; D009599:Nitroprusside; D008734:Methoxyhydroxyphenylglycol; D003847:Deoxyglucose; C010117:3,4-dihydroxyphenylglycol; D009638:Norepinephrine; D004837:Epinephrine