Expression of a human insulin-like growth factor II cDNA in NIH-3T3 cells.
Recombinant human insulin-like growth factor II (IGF-II) was produced in NIH-3T3 cells transfected with a plasmid containing a construct encoding the signal peptide and the sequence of mature human IGF II. Successfully transfected clones secreted correctly processed recombinant human IGF II at rates of about 100ng per 24 hours per 10(6) cells. The biological activity of the purified recombinant human IGF II exhibited similar potencies as standard human IGF II isolated from serum in radio-receptorassays as well as in thymidine incorporation assays.
Buergisser
D M
DM
Department of Biochemistry, University of Zürich, Switzerland.
Roth
B V
BV
Luethi
C
C
Gerber
H P
HP
Honegger
A
A
Humbel
R E
RE
D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D066298:In Vitro Techniques; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D051379:Mice; D008970:Molecular Weight; D011869:Radioligand Assay; D013002:Somatomedins / Q000235:genetics*
D013002:Somatomedins; D007335:Insulin-Like Growth Factor II; D004247:DNA