title: [Selective interaction of antipeptide antibodies to N-terminal site of lactate dehydrogenase-5 from swine muscle with various isoforms of human lactate dehydrogenase].
abstract: Antipeptide antibodies produced towards N-terminal site of lactate dehydrogenase-5 (LDH5) from pig muscular tissue exhibited specific reactions with N-terminal site of pig LDH5 and the whole enzyme molecule. An immunosorbent containing the antipeptide antibodies produced bound and precipitated all the LDH isoforms involving even a single M-subunit of the enzyme (LDH2, LDH5) but did not precipitate H4-isoform (LDH1) in extracts of human heart and skeletal muscle tissues and in blood serum of patients with myocardium infarction. The data obtained suggest that antipeptide antibodies against N-terminal site of pig muscular tissue LDH reacted selectively only with M-subunits of human LDH and did not interact with H4 isoform, which occurred due to dissimilarities in primary structure of N-terminal sites of M4 and H4 LDH isoenzymes.
authors.forename: A E
authors.initials: AE
authors.lastname: Alekseeva
authors.forename: T A
authors.initials: TA
authors.lastname: Potapkina
authors.forename: V N
authors.initials: VN
authors.lastname: Prozorovskiĭ
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D001667:Binding, Competitive; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000276:immunology*; D009132:Muscles / Q000201:enzymology*; D010455:Peptides / Q000276:immunology*; D013045:Species Specificity; D013552:Swine
chemical_list: D000906:Antibodies; D007527:Isoenzymes; D010455:Peptides; D007770:L-Lactate Dehydrogenase
vernacular_title: Izbiratel'noe vzaimodeĭstvie antipeptidnykh antitel k N-kontsevomu raĭonu laktatdegidrogenazy-5 myshechnoĭ tkani svin'i s razlichnymi izoformami laktatdegidrogenazy cheloveka.