OP-1 cDNA encodes an osteogenic protein in the TGF-beta family.
Amino acid sequences of two tryptic peptides derived from enriched bovine osteogenic protein preparations revealed considerable homology to two members of the TGF-beta (transforming growth factor beta) supergene family, DPP (decapentaplegic protein) of Drosophila and Vg-1 (vegetal protein) of Xenopus. Building upon this information we constructed a synthetic consensus gene to use as a probe to screen human genomic libraries. This resulted in the isolation of three interrelated genes. Among these were BMP-2b and BMP-3 which have recently been described by others. The third gene, termed OP-1 (osteogenic protein one), is new and was subsequently shown to encode the human homolog of a major component of bovine osteogenic protein. The genomic clones were used to isolate the corresponding complementary DNA (cDNA) clones. Sequence analysis indicates that OP-1 is a relative of the murine Vgr-1 (Vg-1 related gene). This report describes the cDNA structure and putative amino acid sequence of OP-1.
Ozkaynak
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E
Creative BioMolecules, Inc., Hopkinton, MA 01748.
Rueger
D C
DC
Drier
E A
EA
Corbett
C
C
Ridge
R J
RJ
Sampath
T K
TK
Oppermann
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015342:DNA Probes; D015723:Gene Library; D015698:Genomic Library; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D010012:Osteogenesis; D011498:Protein Precursors / Q000235:genetics; D011506:Proteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D016212:Transforming Growth Factor beta; D015291:Transforming Growth Factors / Q000235:genetics*; D029867:Xenopus Proteins
C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D015342:DNA Probes; D011498:Protein Precursors; D011506:Proteins; D016212:Transforming Growth Factor beta; D029867:Xenopus Proteins; C525822:bmp7.1 protein, Xenopus; D015291:Transforming Growth Factors; D004247:DNA