Sensitive and rapid colorimetric immunoenzymometric assay of ferritin in biological samples.
We describe a rapid and sensitive enzyme-linked immunosorbent assay (ELISA) for quantifying ferritin in human and rat biological fluids. We used chlorophenol red beta-D-galactopyranoside as the colorimetric substrate of beta-galactosidase (EC 3.2.1.23), which is coupled to specific antibodies to either human or rat liver ferritin. The assay is sensitive (detection limit for human assay = 0.58 micrograms/L and for rat assay = 0.37 micrograms/L), accurate (average recovery for human assay = 93% and for rat assay = 92%), and precise (total CVs for human assay = 2.3-12.2% and for rat assay = 5.6-11.3%). The results correlated well with those of an established immunoradiometric technique (r = 0.99691). This assay has a prolonged shelf-life, is inexpensive, and utilizes a stable colorimetric substrate that requires relatively short incubation.
Ramm
G A
GA
Department of Medicine, Royal Brisbane Hospital, Australia.
Duplock
L R
LR
Powell
L W
LW
Halliday
J W
JW
D000818:Animals; D001322:Autoanalysis; D001646:Bile / Q000032:analysis*; D003124:Colorimetry; D004797:Enzyme-Linked Immunosorbent Assay; D005293:Ferritins / Q000032:analysis* / Q000097:blood; D006801:Humans; D015592:Immunoradiometric Assay; D051381:Rats; D015203:Reproducibility of Results
D005293:Ferritins