Formation of a cytosolic ganglioside-protein complex following administration of photoreactive ganglioside GM1 to human fibroblasts in culture.
A GM 1 ganglioside derivative bearing a photoreactive nitrophenyl azide group and tritium labeled at the acetyl group of N-acetylneuraminic acid, has been administered to cultured human fibroblasts. With photolabeling experiments we found that a portion of the ganglioside in the cell cytosol was associated with a soluble protein of about 30 kDa.
Chigorno
V
V
Department of Medical Chemistry and Biochemistry, Medical School, University of Milan, Italy.
Valsecchi
M
M
Acquotti
D
D
Sonnino
S
S
Tettamanti
G
G
D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000378:metabolism*; D005677:G(M1) Ganglioside / Q000378:metabolism*; D006801:Humans; D008970:Molecular Weight; D010777:Photochemistry; D011485:Protein Binding
D005677:G(M1) Ganglioside