The effects of 2,3-butanedione monoxime on initial heat, tension, and aequorin light output of ferret papillary muscles.
At low concentrations (up to 5 mM) the compound 2,3-butanedione monoxime (BDM) was found to reduce twitch tension and initial heat production in isolated papillary muscles without significantly affecting the size of the intracellular Ca2+ transient measured with aequorin luminescence. Higher concentrations of BDM caused further inhibition of twitch tension and heat production with a fall in the size of the Ca2+ transient. The size of the aequorin transient was 50% of the control value at 15 mM BDM while twitch tension was negligible. These results suggest that BDM selectively inhibits Ca2+ activated force in cardiac muscle at low concentrations with additional effects on intra-cellular calcium at concentrations above 5 mM.
Blanchard
E M
EM
Department of Physiology, University of Vermont, Burlington 05405.
Smith
G L
GL
Allen
D G
DG
Alpert
N R
NR
D000331:Aequorin / Q000378:metabolism; D000818:Animals; D001833:Body Temperature Regulation / Q000187:drug effects; D002074:Butanones / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002801:Cholinesterase Reactivators / Q000494:pharmacology*; D003931:Diacetyl / Q000031:analogs & derivatives / Q000494:pharmacology*; D005289:Ferrets; D008027:Light; D009200:Myocardial Contraction / Q000187:drug effects*; D010210:Papillary Muscles / Q000378:metabolism*
D002074:Butanones; D002801:Cholinesterase Reactivators; C004717:diacetylmonoxime; D000331:Aequorin; D003931:Diacetyl; D002118:Calcium