title: Lipid diet and enterocyte microsomal membrane fluidity in rats.
abstract: Rats were fed on diets more or less enriched with n-3 and n-6 unsaturated fatty acids, before removal of the small intestine. The global protein, cholesterol and phospholipid contents of enterocyte microsomes were measured. Fatty acids of the total lipid extracts were determined. Acyl coenzyme A: cholesterol acyl transferase (ACAT) was chosen as the enzyme whose activity reflects metabolic changes induced by lipid diets. Fluorescence measurements using diphenylhexatriene as the membrane probe were performed. As dietary fat may change the fatty acid composition of membranes, the order parameter S calculated from fluorescence measurements was studied with regard to dietary fatty acid composition. The S values, distributed over a large range, were not different between rat groups. They were positively correlated with the ratios of cholesterol and proteins to phospholipids and the molar percentage of saturated fatty acids. ACAT activity was negatively correlated with S. Variations in S values among rats, whatever the diet, could in part be attributed to individual factors.
authors.affiliation: Unité INSERM U 130, Marseille, France.
authors.forename: M
authors.initials: M
authors.lastname: Chautan
authors.forename: M
authors.initials: M
authors.lastname: Dell'Amico
authors.forename: M
authors.initials: M
authors.lastname: Bourdeaux
authors.forename: J
authors.initials: J
authors.lastname: Leonardi
authors.forename: M
authors.initials: M
authors.lastname: Charbonnier
authors.forename: H
authors.initials: H
authors.lastname: Lafont
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D004041:Dietary Fats / Q000378:metabolism*; D004847:Epithelial Cells; D005227:Fatty Acids / Q000378:metabolism; D007422:Intestines / Q000166:cytology / Q000648:ultrastructure*; D007425:Intracellular Membranes / Q000201:enzymology; D008055:Lipids / Q000494:pharmacology*; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects*; D008861:Microsomes / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D002785:Sterol O-Acyltransferase / Q000378:metabolism
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids; D008055:Lipids; D011506:Proteins; D002784:Cholesterol; D002785:Sterol O-Acyltransferase