title: Transferrin polymorphism detected in human urine using isoelectric focusing followed by immunoblotting.
abstract: Genetic polymorphism of transferrin (TF) was revealed in human urine by isoelectric focusing and immunoblotting on thin-layer polyacrylamide gels. Using this technique more than 300 urine samples were examined, and correct TF typing from a small volume of urine (approx. 0.5 ml) was achieved, in comparison with the results of direct grouping for plasma. Three common phenotypes, TF C1, C2-1 and C2, were differentiated. In addition, the rare types TF C1D, C2D, and C1B were observed. The frequencies of the TF alleles in our samples were found to be: TF*C1 = 0.7265, TF*C2 = 0.2624, TF*D = 0.0083 and TF*B = 0.0028.
authors.affiliation: Department of Legal Medicine, Fukui Medical School, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kishi
authors.forename: Y
authors.initials: Y
authors.lastname: Ikehara
authors.forename: T
authors.initials: T
authors.lastname: Yasuda
authors.forename: K
authors.initials: K
authors.lastname: Mizuta
authors.forename: W
authors.initials: W
authors.lastname: Sato
mesh_terms: D000483:Alleles; D015925:Cryopreservation; D006801:Humans; D015151:Immunoblotting; D007525:Isoelectric Focusing; D007564:Japan; D010641:Phenotype; D011110:Polymorphism, Genetic; D014168:Transferrin / Q000235:genetics / Q000652:urine*
chemical_list: D014168:Transferrin