A DNA-binding protein is required for termination of transcription by RNA polymerase I in Xenopus laevis.
We describe a partially fractionated in vitro transcription system from Xenopus laevis for the assay of transcription termination by RNA polymerase I. Termination in vitro was found to require a specific terminator sequence in the DNA and a DNA-binding protein fraction that produces a footprint over the terminator sequence.
McStay
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B
Basic Sciences Division, Hutchinson Cancer Research Center, Seattle, Washington 98104.
Reeder
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RH
D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D003850:Deoxyribonuclease I; D005786:Gene Expression Regulation; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010957:Plasmids; D012318:RNA Polymerase I / Q000378:metabolism*; D015183:Restriction Mapping; D013698:Templates, Genetic; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic; D014982:Xenopus laevis
D004268:DNA-Binding Proteins; D015345:Oligonucleotide Probes; D012318:RNA Polymerase I; D003850:Deoxyribonuclease I