title: Exon 2 DNA sequence of the HLA-DRw13b allele obtained from genomes of five different individuals.
abstract: Exon 2 nucleotide sequence of the DRB1 gene encoding the HLA-DRw13b allele defined by DNA-RFLP (Restriction Fragment Length Polymorphism) typing, has been obtained by using five heterozygous individuals genomic DNA and a non isotopic automated "dideoxi" methodology. Its comparison with other known homologous DRB1 sequences suggests that two different mechanisms which generate HLA allele variability may have occurred in this particular exon 2: a gene conversion between DRw11 or DRw13 as acceptors and DR4-Dw15 or DRw8.1 as donors and in addition, a non-conservative point mutation at base 221. The relationship between this HLA sequence characteristics and certain diseases susceptibility is discussed.
authors.affiliation: Department of Immunology, Hospital Doce de Octubre, Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Corell
authors.forename: J M
authors.initials: JM
authors.lastname: Martin-Villa
authors.forename: P
authors.initials: P
authors.lastname: Varela
authors.forename: J L
authors.initials: JL
authors.lastname: Vicario
authors.forename: J
authors.initials: J
authors.lastname: Martinez-Laso
authors.forename: A
authors.initials: A
authors.lastname: Arnaiz-Villena
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D005091:Exons / Q000502:physiology*; D015894:Genome, Human; D006684:HLA-DR Antigens / Q000235:genetics*; D059810:HLA-DR Serological Subtypes; D006801:Humans; D008969:Molecular Sequence Data
chemical_list: D006684:HLA-DR Antigens; D059810:HLA-DR Serological Subtypes; C097337:HLA-DR13 antigen; D004247:DNA