title: [NAD-dependent formate dehydrogenase from methylotrophic bacteria Pseudomonas sp. 101. I. Amino acid sequence].
abstract: The primary structure of NAD-dependent formate dehydrogenase from methylotrophic bacterium Pseudomonas sp. 101 is determined. The enzyme is composed of two identical subunits, each comprising 393 amino acid residues, and has a molecular weight of 43.1 kD. To elucidate the protein's amino acid sequence, four types of digestion were used: cyanogen bromide cleavage at methionine residues, endoproteinase Lys-C digestion at lysine residues, endoproteinase Glu-C cleavage at glutamic acid residues, and tryptic digestion. The peptides obtained were purified to homogeneity and characterized.
authors.forename: V O
authors.initials: VO
authors.lastname: Popov
authors.forename: I A
authors.initials: IA
authors.lastname: Shumilin
authors.forename: T B
authors.initials: TB
authors.lastname: Ustinnikova
authors.forename: V S
authors.initials: VS
authors.lastname: Lamzin
authors.forename: Ts A
authors.initials: TsA
authors.lastname: Egorov
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000032:analysis*; D000595:Amino Acid Sequence; D005560:Formate Dehydrogenases / Q000032:analysis* / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011549:Pseudomonas / Q000201:enzymology*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D005560:Formate Dehydrogenases; D000445:Aldehyde Oxidoreductases
vernacular_title: NAD-zavisimaia formiatdegidrogenaza metilotrofnykh bakteriĭ Pseudomonas sp. 101. I. Aminokislotnaia posledovatel'nost'.