Detection and characterization of circulating antigens in acute experimental infections of mice with four different strains of Toxoplasma gondii.
Acute infections with four different strains of Toxoplasma gondii, all of them being highly pathogenic for mice, were provoked by intraperitoneal injection of 2.2 x 10(7) trophozoites. The times of the appearance of circulating antigens in the sera of the infected mice were determined and the amounts of these antigens were measured by an enzyme-linked immunosorbent assay. The molecular weight of the circulating antigens was determined by gel filtration and by Western blot following PAGE. The isoelectric points of these antigens were determined by immunoblotting after isoelectric focusing. Circulating antigens were detectable up to ng amounts/ml serum from the 1st day p.i. onwards. The circulating antigens consisted of a number of proteins with molecular weights of greater than 10(4), 300, 65, 25, and less than 5 kd. The isoelectric points of these proteins were situated between pH 3.5 and pH 7. Time of appearance and structure of the circulating antigens were very similar in all four Toxoplasma strains. Circulating antigens are apparently generated regularly in experimental acute Toxoplasma infections, and lysis of parasite cells appears to be the major mechanism of their formation.
Hassl
A
A
Abteilung für Medizinische Parasitologie, Universität Wien.
Aspöck
H
H
D000818:Animals; D000953:Antigens, Protozoan / Q000097:blood*; D015153:Blotting, Western; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007525:Isoelectric Focusing; D051379:Mice; D008970:Molecular Weight; D014122:Toxoplasma / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000276:immunology*
D000953:Antigens, Protozoan