Stimulation of collagen synthesis in fibroblast cultures by a triterpene extracted from Centella asiatica.
The drug "Titrated Extract from Centella asiatica" (TECA), used for its stimulating properties on the healing of wounds, is a mixture of 3 terpenes extracted from a tropical plant: asiatic acid (30%, w/w), madecassic acid (30%, w/w) and asiaticoside (40%, w/w). The effects of TECA and its individual components were checked on human foreskin fibroblast monolayer cultures. TECA increased the collagen synthesis in a dose-dependent fashion whereas a simultaneous decrease in the specific activity of neosynthesized collagen was observed. Asiatic acid was found to be the only component responsible for collagen synthesis stimulation. TECA and all three terpenes increased the intracellular free proline pool. This effect was independent of the stimulation of collagen synthesis.
Maquart
F X
FX
Laboratory of Biochemistry, CNRS URA, Faculty of Medicine, Reims, France.
Bellon
G
G
Gillery
P
P
Wegrowski
Y
Y
Borel
J P
JP
D002452:Cell Count; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D006909:Hydroxyproline / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D008297:Male; D053978:Pentacyclic Triterpenes; D011347:Procollagen / Q000378:metabolism; D011392:Proline / Q000378:metabolism; D012361:RNA, Transfer, Pro / Q000378:metabolism; D013729:Terpenes / Q000494:pharmacology*; D014315:Triterpenes / Q000494:pharmacology*
D053978:Pentacyclic Triterpenes; D011347:Procollagen; D012361:RNA, Transfer, Pro; D013729:Terpenes; D014315:Triterpenes; D003094:Collagen; D011392:Proline; C017032:asiatic acid; D007930:Leucine; C001669:madecassic acid; C004446:asiaticoside; D006909:Hydroxyproline