Excision-repair capacity in Streptococcus pneumoniae: cloning and expression of a uvr-like gene.
Although deficient in photoreactivation and some SOS-like functions, Streptococcus pneumoniae has the capacity to carry out excision repair when exposed to UV light. The repair ability and sensitivity to UV irradiation or treatment with chemical agents in the wild type and a UV-sensitive mutant strain indicate that UV-induced pyrimidine dimers might be repaired in pneumococcus by a system similar to the uvr-dependent system in Escherichia coli. A gene complementing the mutation conferring UV sensitivity of the mutant strain has been cloned. The coding region directs the synthesis of a polypeptide with a molecular weight of 78 kDa. The relationship with uvr-like protein in E. coli is discussed.
Sicard
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Centre de Recherche de Biochimie et de Génétique Cellulaires du CNRS, Toulouse, France.
Estevenon
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D001426:Bacterial Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004260:DNA Repair; D004269:DNA, Bacterial / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D015870:Gene Expression; D009154:Mutation; D011740:Pyrimidine Dimers / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D015183:Restriction Mapping; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D011740:Pyrimidine Dimers; D011994:Recombinant Proteins