Physical characterization of histidine-rich protein from Plasmodium lophurae.
The size and shape of Plasmodium lophurae histidine-rich protein have been determined by analytical centrifugation and electron microscopy. From the partial specific volume of 0.72 cc/g, the molecular weight was determined to be 43,000. The sedimentation velocity studies indicated a coefficient of 1.32 S in 0.9 M acetic acid (pH 3.5), monodispersity and significant asymmetry. Darkfield electron microscopy revealed the major species to be compact oblate spheroids 12 nm in width and extended filamentous particles of average length 35 nm by 1.5 nm. Analysis of the sequence of the protein by the method of Garnier et al. (J. Mol. Biol. (1978) 120, 97-120) predicted that 82% of its residues would be found in three long alpha-helices. The protein's CD spectrum has a strong resemblance to that of poly(L-histidine) at pH 4-5, where the homopolymer is thought to be in a right-handed alpha-helical form. A single helix containing 300 residues would be 45 nm long, the largest length found by electron microscopy. From the electron-microscopic data, sedimentation coefficients of 1.6 and 1.95 S, respectively, were calculated for flexible-coil and extended-rod models, in closer agreement with the measured value of 1.3 S than the value calculated for a spherical model. Thus, the major species in acetic acid is probably an incompletely extended rod which, as the pH is increased to neutrality, condenses to form spherical molecular aggregates seen in the malaria parasite.
Margossian
S S
SS
Department of Biochemistry, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, NY.
McPhie
P
P
Howard
R J
RJ
Coligan
J E
JE
Slayter
H S
HS
D000818:Animals; D002498:Centrifugation; D002942:Circular Dichroism; D001330:Electronic Data Processing; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010961:Plasmodium / Q000032:analysis* / Q000528:radiation effects / Q000648:ultrastructure; D011487:Protein Conformation; D011506:Proteins / Q000032:analysis* / Q000648:ultrastructure; D015800:Protozoan Proteins / Q000032:analysis* / Q000648:ultrastructure; D014414:Tungsten; D014466:Ultraviolet Rays; D000099053:Histidine-Rich Glycoprotein
D011506:Proteins; D015800:Protozoan Proteins; D000099053:Histidine-Rich Glycoprotein; D014414:Tungsten