title: Tissue distribution of 131I radiolabeled transferrin in the athymic nude mouse: localization of a human colon adenocarcinoma HT-29 xenograft.
abstract: The tissue distribution of 131I-transferrin (131I-Tf) was studied in athymic nude mice having s.c. human colonic adenocarcinoma HT-29 xenografts. Four days after 131I-Tf injection, the 131I-specific activity measured in the HT-29 tumor, i.e. amount of radioactivity per gram of fresh tissue, represented 0.31 +/- 0.09% of the injected radioactivity and was 1.90 fold more than that measured in the murine colon (P less than 0.05). After correction for intravascular 131I-Tf as estimated by means of 99mTc-Sn in vivo labeling of red blood cells, the 131I specific activity observed in the HT-29 tumor was 7.21 fold more than that observed in the murine colon. This subtracting method enabled us to localize a HT-29 tumor xenograft by gamma scintigraphy of the entire animal and demonstrated that 131I-Tf could be a non-specific but potent marker for human colon cancer.
authors.affiliation: Service de Biophysique, C.H.U. La Pitié-Salpêtrière, Paris, France.
authors.forename: A C
authors.initials: AC
authors.lastname: Prost
authors.forename: M
authors.initials: M
authors.lastname: Anakök
authors.forename: A
authors.initials: A
authors.lastname: Aurengo
authors.forename: J C
authors.initials: JC
authors.lastname: Salomon
authors.forename: J C
authors.initials: JC
authors.lastname: Legrand
authors.forename: G
authors.initials: G
authors.lastname: Rosselin
mesh_terms: D000230:Adenocarcinoma / Q000000981:diagnostic imaging*; D000818:Animals; D003110:Colonic Neoplasms / Q000000981:diagnostic imaging*; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011877:Radionuclide Imaging; D014018:Tissue Distribution; D014168:Transferrin / Q000493:pharmacokinetics*; D014183:Transplantation, Heterologous
chemical_list: D007457:Iodine Radioisotopes; D014168:Transferrin