A cholecystokinin-metabolizing enzyme in rat intestine.
Acid extracts of rat intestine contain a material which metabolizes cholecystokinin-33 (CCK-33) to CCK-12. Soybean trypsin inhibitor had little effect on CCK metabolism by the intestinal material. The molecular weight of the CCK-metabolizing activity, estimated by gel filtration, was 34,000. These data suggest rat intestine contains a nontrypsin CCK-metabolizing enzyme. Results from gel filtration also suggest that large CCK forms can be artifactually degraded to smaller ones during chromatography.
Turkelson
C M
CM
Veterans Administration Medical Center, Kansas City, MO 64128.
Solomon
T E
TE
Hamilton
J
J
D000595:Amino Acid Sequence; D000818:Animals; D002766:Cholecystokinin / Q000096:biosynthesis* / Q000378:metabolism; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D007421:Intestine, Small / Q000032:analysis / Q000201:enzymology*; D008297:Male; D008970:Molecular Weight; D011499:Protein Processing, Post-Translational; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D002766:Cholecystokinin