title: Unusual stability properties of a reptilian ceruloplasmin.
abstract: Ceruloplasmin from the turtle Caretta caretta was isolated to purity by using the single-step procedure recently developed by us to purify sheep and chicken ceruloplasmin. It has a Mr of ca. 145,000 and a total copper content of 5.1 +/- 0.2 atoms of copper per molecule, 50% of which are detectable by EPR. The spectroscopic features include an absorption maximum at 603 nm in the electronic spectrum and the total absence of any resonance attributable to Type 2 copper in the EPR spectrum. Turtle ceruloplasmin was found to be unusually resistant to aging and proteolysis, when compared to ceruloplasmins isolated from other species. p-Phenyl-endiamine oxidase activity measurements revealed an unusually low catalytic efficiency, while the kinetic parameters of Fe(II) oxidation were consistent with those reported for other species of ceruloplasmin.
authors.affiliation: Center of Molecular Biology of C.N.R., University of Rome, La Sapienza, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Musci
authors.forename: M
authors.initials: M
authors.lastname: Carbonaro
authors.forename: A
authors.initials: A
authors.lastname: Adriani
authors.forename: A
authors.initials: A
authors.lastname: Lania
authors.forename: A
authors.initials: A
authors.lastname: Galtieri
authors.forename: L
authors.initials: L
authors.lastname: Calabrese
mesh_terms: D000818:Animals; D002570:Ceruloplasmin / Q000302:isolation & purification*; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D012756:Sheep; D014426:Turtles / Q000502:physiology*
chemical_list: D002570:Ceruloplasmin