Identification of a G1-S-phase-regulated region in the human thymidine kinase gene promoter.
We have identified a regulatory region in the human thymidine kinase gene promoter. A set of promoter deletion mutants was constructed, linked to the bacterial neomycin resistance gene, and stably transfected into Rat3 cells. It was shown that the region between 135 and 67 base pairs upstream of the cap site is required for conveying G1-S-phase regulation to the linked neo gene. In addition, primer extension assays demonstrated that the same transcriptional start sites were used in G1- and S-phase cells and in the various deletion mutants tested.
Roehl
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HH
Department of Microbiology and Public Health, Michigan State University, East Lansing 48824-1101.
Conrad
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D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002872:Chromosome Deletion; D015971:Gene Expression Regulation, Enzymologic; D005796:Genes; D006801:Humans; D007399:Interphase; D008969:Molecular Sequence Data; D009154:Mutation; D015345:Oligonucleotide Probes; D011401:Promoter Regions, Genetic; D051381:Rats; D013937:Thymidine Kinase / Q000235:genetics*; D014162:Transfection
D015345:Oligonucleotide Probes; D013937:Thymidine Kinase