Counter-current chromatography of black tea infusions.
Counter-current chromatography using a multilayer coil planet centrifuge, with solvent system ethyl acetate-butanol-water, permits the separation of black tea infusions into fractions which include pure SII and a mixture of SI and SIa thearubigins. Good resolution of several components of the infusion may be achieved in elution times of 1 to 2 h. The appearance of chromatograms is altered on decaffeinating the infusion. The effect of stationary phase composition is considered. Resolution of the peaks improves with butanol content.
Wedzicha
B L
BL
Procter Department of Food Science, University of Leeds, U.K.
Lo
M F
MF
Donovan
T J
TJ
D002853:Chromatography, Liquid / Q000379:methods; D010936:Plant Extracts / Q000032:analysis; D013662:Tea / Q000032:analysis*
D010936:Plant Extracts; D013662:Tea