Simple method for the analysis of glycerol enol ethers derived from plasmalogens in complex lipid mixtures and subsequent determination of the aldehydic components by gas chromatography-mass spectrometry.
Glycerol enol ethers, obtained by the reduction of plasmalogens with lithium aluminum hydride, can be converted into glycerol alk-(1)-enyl ether bismethyl ethers with diazomethane in the presence of silica gel. Their mass spectra allow the position of the enol ether group in glycerol unit to be deduced. Branches in the aldehydic components of the glycerol alk-(1)-enyl ether bismethyl ethers can be identified unequivocally by preparation of the 2-alkyl-1,3-dithiolanes, desulphurization with Raney nickel and deuterium to hydrocarbons and subsequent analysis by gas chromatography-mass spectrometry.
Knörr
W
W
Lehrstuhl für Organische Chemie I, Universität Bayreuth, F.R.G.
Spiteller
G
G
D000447:Aldehydes / Q000032:analysis*; D000818:Animals; D002417:Cattle; D003978:Diazomethane; D008401:Gas Chromatography-Mass Spectrometry; D005995:Glyceryl Ethers / Q000138:chemical synthesis*; D008099:Liver / Q000032:analysis; D008738:Methyl Ethers / Q000138:chemical synthesis*; D010084:Oxidation-Reduction; D010742:Phospholipid Ethers / Q000032:analysis; D010955:Plasmalogens
D000447:Aldehydes; D005995:Glyceryl Ethers; D008738:Methyl Ethers; D010742:Phospholipid Ethers; D010955:Plasmalogens; D003978:Diazomethane