Laser desorption electron impact: application to a study of the mechanism of conjugation of glutathione and cyclophosphamide.
Toward the objective of producing ion radical species from involatile and thermally labile samples, we have combined laser desorption of neutral molecules with electron impact ionization on a time-of-flight mass analyzer with a delayed draw-out pulse. The analytical capabilities of this method are tested in the analysis of isotope labels in the involatile product in a mechanistic study of both the chemical and the enzyme catalyzed reactions of cyclophosphamide with glutathione.
Yuan
Z M
ZM
Department of Chemistry and Biochemistry, University of Maryland Baltimore County 21228.
Fenselau
C
C
Dulik
D M
DM
Martin
W
W
Emary
W B
WB
Brundrett
R B
RB
Colvin
O M
OM
Cotter
R J
RJ
D000818:Animals; D003520:Cyclophosphamide / Q000378:metabolism; D003903:Deuterium; D004583:Electrons; D005978:Glutathione / Q000378:metabolism; D007834:Lasers; D013058:Mass Spectrometry / Q000379:methods; D011817:Rabbits; D013057:Spectrum Analysis / Q000379:methods
D003520:Cyclophosphamide; D003903:Deuterium; D005978:Glutathione