Flow cytometric analysis of cellular DNA content in ovarian cancer.
A total of 169 paraffin-embedded tissue sections from 42 patients with epithelial ovarian cancer were subjected to analysis of cellular DNA content by flow cytometry. Twenty-three cases were found to be homogenously diploid, whereas 19 cases were aneuploid. A "mosaic" type containing both aneuploid and diploid cell populations was found in 11 of 19 aneuploid cases. Clinical features showed significant correlation with tumor ploidy of FIGO stage and bulky disease. In evaluation of the prognostic value of tumor ploidy, "mosaic" tumors were frequently observed in women who died of disease (DOD), whereas women with diploid tumors survived longer than those with "mosaic" tumors. The present results suggest that determination of DNA heterogeneity may be a valuable parameter in the prognosis of epithelial ovarian cancer.
Hamaguchi
K
K
Department of Obstetrics and Gynecology, Kurume University School of Medicine, Kurume, Japan.
Nishimura
H
H
Miyoshi
T
T
Miyahara
K
K
Tateno
N
N
Yakushiji
M
M
Yokoyama
M M
MM
D004247:DNA / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D009030:Mosaicism; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D011003:Ploidies
D004247:DNA