Cellular fatty acid compositions of an unidentified organism and a bacterium associated with cat scratch disease.
The cellular fatty acid composition of a gram-negative bacterium associated with cat scratch disease was determined by capillary gas chromatography-mass spectrometry (GC-MS). The fatty acid profile of this organism was distinct from those of other bacteria we have tested and was characterized by an unknown acid which was identified as 11-methyloctadec-12-enoic acid. The position of the branched methyl group in this acid was established by GC-MS of the reduced acid, and the location of the double bond was confirmed by GC-MS analysis of dimethyl disulfide derivatives. Another clinical isolate with no known relationship to cat scratch disease but with similar morphological and biochemical features had a similar fatty acid profile, including 11-methyloctadec-12-enoic acid.
Moss
C W
CW
Analytical Chemistry Laboratory, Division of Bacterial Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
Holzer
G
G
Wallace
P L
PL
Hollis
D G
DG
D002372:Cat-Scratch Disease / Q000382:microbiology*; D005227:Fatty Acids / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D006090:Gram-Negative Bacteria / Q000032:analysis* / Q000302:isolation & purification; D006801:Humans; D009829:Oleic Acids / Q000032:analysis
D005227:Fatty Acids; D009829:Oleic Acids; C064523:11-methyloctadeca-12-enoic acid