Cloning and heterospecific expression of the resistance determinant vanA encoding high-level resistance to glycopeptides in Enterococcus faecium BM4147.
Fragments of plasmid pIP816, which confers high-level glycopeptide resistance in Enterococcus faecium BM4147, were cloned into a conjugative gram-negative-gram-positive shuttle vector. The resulting hybrids were transferred by conjugation from Escherichia coli to Enterococcus faecalis and Bacillus thuringiensis. A 4-kilobase EcoRI fragment from pIP816 was found to confer vancomycin resistance in these hosts but not in E. coli or Bacillus subtilis.
Brisson-Noël
A
A
Unité des Agents Antibactériens, Unité Associée Centre National de la Recherche Scientifique 271, Institut Pasteur, Paris, France.
Dutka-Malen
S
S
Molinas
C
C
Leclercq
R
R
Courvalin
P
P
D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D004352:Drug Resistance, Microbial; D015964:Gene Expression Regulation, Bacterial; D006020:Glycopeptides / Q000494:pharmacology*; D010957:Plasmids; D013291:Streptococcus / Q000187:drug effects / Q000235:genetics*; D014640:Vancomycin / Q000494:pharmacology*
D006020:Glycopeptides; D014640:Vancomycin