Restriction site mapping of adenovirus prototypes of subgenus D.
Restriction site maps with the endonucleases HindIII, BglII, and Bam HI were elaborated for the majority of 23 adenovirus prototypes of subgenus D (AV8 to AV39). On the basis of four or five types which have been mapped by biochemical methods, the physical maps for other types were constructed by adapting restriction fragments of defined molecular weight, as the homology among types of the same subgenus is considerable. Prototypes of subgenus D shared several common restriction sites; different sites, however, were distributed at random over the genome. The gene loci for hexon and fiber did not show either higher or lower variability.
Wigand
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R
Abteilung für Virologie, Institut für Medizinische Mikrobiologie und Hygiene, Universitätskliniken, Homburg (Saar), Federal Republic of Germany.
Adrian
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T
D000256:Adenoviridae / Q000235:genetics*; D001426:Bacterial Proteins; D004279:DNA, Viral / Q000032:analysis*; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D014644:Genetic Variation; D006801:Humans; D008970:Molecular Weight; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D001426:Bacterial Proteins; D004279:DNA, Viral; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific