title: Chemical synthesis and characteristics of a hybrid phage T5-lac promoter.
abstract: A new P1O1R9 inducible promoter (where P1 is a promoter sequence analogous to that of the phage T5 early promoter; O1 is lac-operator; and R9 is a ribosome binding site) was synthesized. We studied the efficiency of this promoter in controlling and inducible gene expression using two model genes: human calcitonin tetrameric (hCT[4]) and human interferon alpha 1 (hIFN alpha 1). The synthetic lac-operator O1 was found to repress P1 activity in media free of lac-operon inducers which was derepressed in the presence of IPTG. The levels of expression of both genes (evaluated by quantitating mRNA and protein) in the presence of lac-inducers were close to those obtained with the P1R9 constitutive promoter.
authors.affiliation: Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
authors.forename: I
authors.initials: I
authors.lastname: Ivanov
authors.forename: J
authors.initials: J
authors.lastname: Rommens
authors.forename: A
authors.initials: A
authors.lastname: Sarafova
authors.forename: V
authors.initials: V
authors.lastname: Maximova
authors.forename: A
authors.initials: A
authors.lastname: Usheva
authors.forename: S
authors.initials: S
authors.lastname: Bardarov
authors.forename: A
authors.initials: A
authors.lastname: Papageorgiou
authors.forename: E
authors.initials: E
authors.lastname: Jay
mesh_terms: D001483:Base Sequence; D002116:Calcitonin / Q000235:genetics*; D003001:Cloning, Molecular; D015967:Gene Expression Regulation, Viral; D006801:Humans; D007370:Interferon Type I / Q000235:genetics*; D007700:Kinetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D013604:T-Phages / Q000235:genetics*
chemical_list: D007370:Interferon Type I; D002116:Calcitonin