[Monoclonal antibodies IPO-4 recognizing antigen-activated human T and B lymphocytes].
Spleen cells from BALB/c mice previously immunized with B-lymphoblastoid cell line RPMI-1788 were fused with P3-X-63-Ag8.653 myeloma cells. Monoclonal antibodies (Ab) IPO-4 were screened on 18 cell lines by the indirect immuno-fluorescence method. Cryostat sections of tissues were stained according to the PAP technique. The Mab IPO-4 were tested for reactivity with blood cells of 17 healthy persons and 102 patients with chronic lymphocytic leukemia, acute lymphoblastic and myeloblastic leukemias, hairy cell leukemia, multiple myeloma, non-Hodgkin's lymphomas and Hodgkin's disease and mitogen stimulated lymphocytes. MAb IPO-4 were found to be directed against antigen expressed on activated T and B cells.
Sidorenko
S P
SP
Berdova
A G
AG
Vetrova
E P
EP
Shlapatskaia
L N
LN
Iurchenko
O V
OV
Evsev'eva
A I
AI
Gluzman
D F
DF
D000818:Animals; D000911:Antibodies, Monoclonal; D000944:Antigens, Differentiation, B-Lymphocyte / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D014408:Biomarkers, Tumor; D002460:Cell Line; D006801:Humans; D007938:Leukemia / Q000175:diagnosis / Q000276:immunology; D008213:Lymphocyte Activation; D008223:Lymphoma / Q000175:diagnosis / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009101:Multiple Myeloma / Q000175:diagnosis / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000944:Antigens, Differentiation, B-Lymphocyte; D000945:Antigens, Differentiation, T-Lymphocyte; D014408:Biomarkers, Tumor
Monoklonal'nye antitela IPO-4, raspoznaiushchie antigen-aktirovannye T- i B-limfotsity cheloveka.