Mutagenicity evaluation of HC Blue No. 1 and HC Blue No. 2, I. Effect on the induction of micronuclei in mouse bone marrow cells.
Two hair-dye chemicals, HC Blue No. 1 and HC Blue No. 2, were assessed for the ability to produce chromosome breakage and/or spindle malformation in vivo by evaluating the capacity of these compounds to induce micronuclei in polychromatic erythrocytes of mouse bone marrow. Initial studies were conducted in ICR male and female mice given a single intraperitoneal dose of 1000, 500 or 250 mg/kg body weight and examined for micronucleus induction 24 or 48 h later. Activity was observed in female mice given 1000 mg/kg of HC Blue No. 1 at the 24-h harvest time. A questionable response was noted with HC Blue No. 2 in males at the 1000 mg/kg, 24-h time point. No activity was observed in either sex at the 48-h harvest time. In a second set of studies, mice from two strains, ICR and CD-1, were administered a single intraperitoneal dose of 1000 mg/kg of each chemical and the bone marrow was extracted 24 h later. In these experiments, HC Blue No. 1 again produced a statistically significant elevation of micronuclei in female ICR mice. No significant effect was observed in CD-1 mice of either sex. HC Blue No. 2 did not produce any significant elevation of micronuclei in either sex of ICR or CD-1 mice.
Parton
J W
JW
Toxicology Division, Lilly Research Laboratories, A Division of Eli Lilly and Company, Greenfield, IN 46140.
Beyers
J E
JE
Garriott
M L
ML
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000648:ultrastructure; D005260:Female; D006202:Hair Dyes / Q000633:toxicity*; D006203:Hair Preparations / Q000633:toxicity*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D015162:Micronucleus Tests; D009153:Mutagens; D010655:Phenylenediamines / Q000633:toxicity*; D013045:Species Specificity
D006202:Hair Dyes; D006203:Hair Preparations; D009153:Mutagens; D010655:Phenylenediamines; C007906:N-methylamino-2-nitro-4-N',N'-bis(2-hydroxyethyl)aminobenzene; C053869:HC Blue No. 2