FMRF-NH2-like mammalian peptide precipitates opiate-withdrawal syndrome in the rat.
Yang et al. (14) have isolated from mammalian brain an octapeptide FLFQPQRF-NH2 (F-8-F-NH2) with certain antiopiate properties. Third ventricular injection of 2 micrograms of this peptide together with the aminopeptidase inhibitor bestatin precipitated an opiate-withdrawal syndrome in morphine-dependent but not in nondependent rats. Third ventricular injection in nondependent rats of 15 micrograms of the peptide together with bestatin induced a morphine-withdrawal-like behavioral syndrome. This syndrome was not produced by injection of bestatin or saline vehicle alone and was preventable by injection of 3.5 mg/kg morphine sulphate SC.
Malin
D H
DH
University of Houston-Clear Lake, TX 77058.
Lake
J R
JR
Fowler
D E
DE
Hammond
M V
MV
Brown
S L
SL
Leyva
J E
JE
Prasco
P E
PE
Dougherty
T M
TM
D000704:Analysis of Variance; D000818:Animals; D004311:Double-Blind Method; D007276:Injections, Intraventricular; D007930:Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D008297:Male; D009020:Morphine / Q000009:adverse effects* / Q000494:pharmacology; D009479:Neuropeptides / Q000008:administration & dosage / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D009842:Oligopeptides / Q000008:administration & dosage / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D011919:Rats, Inbred Strains; D013375:Substance Withdrawal Syndrome / Q000209:etiology*
D009479:Neuropeptides; D009842:Oligopeptides; D009020:Morphine; C053651:phenylalanyl-leucyl-phenylalanyl-glutaminyl-prolyl-glutaminyl-arginyl-phenylalaninamide; D007930:Leucine; C012211:ubenimex