Introns in histone genes alter the distribution of 3' ends.
Chimeric genes were constructed which contained either a histone or globin promoter, a human alpha-globin coding region as a cDNA or containing one or both intervening sequences, and the 3' end of a mouse histone H2a gene. The genes were introduced into mouse L cells or Chinese Hamster Ovary cells. The genes containing at least one intervening sequence produced two mRNAs in about equal amounts, one which ended at a cryptic polyadenylation site 33 nucleotides 3' to the normal histone mRNA 3' end and one which ended at the normal histone 3' end. In contrast, the same construct containing a globin cDNA yielded mRNA ending only at the correct histone 3' end. Similar proportions of polyadenylated and non-polyadenylated mRNA were obtained when the cryptic polyadenylation signal was replaced with the globin polyadenylation signal. More than 90% of the transcripts were accurately spliced. All of the unspliced transcripts had histone 3' ends.
Pandey
N B
NB
Department of Chemistry, Florida State University, Tallahassee 32306.
Chodchoy
N
N
Liu
T J
TJ
Marzluff
W F
WF
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002678:Chimera / Q000235:genetics; D003001:Cloning, Molecular; D006224:Cricetinae; D005796:Genes; D005914:Globins / Q000235:genetics; D006657:Histones / Q000235:genetics*; D006801:Humans; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D011061:Poly A; D011401:Promoter Regions, Genetic; D012326:RNA Splicing; D014158:Transcription, Genetic
D006657:Histones; D011061:Poly A; D005914:Globins