31P nuclear magnetic resonance studies of growth inhibition and dexamethasone resistance in human leukemic cells.
31P Nuclear magnetic resonance spectra of perchloric acid extracts from wild-type human leukemic CEM-C7 cells and the dexamethasone-resistant CEM-C1 mutant reveal significant differences in concentrations of phospholipid precursors and ATP+, which indicate metabolic differences between these two cell lines. At high cell concentrations the CEM-C7 cells are growth inhibited, which is reflected by low phospholipid precursor levels, indicative of low phospholipid turnover. The CEM-C1 mutant does not exhibit this growth inhibition and has constant phospholipid precursor levels over the same cell concentration range. Dexamethasone causes phospholipid precursor and ATP levels in CEM-C7 to drop after 48 h, but spectra obtained for CEM-C1 cells continue to show high cell viability up to 72 h.
Post
J F
JF
Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston 77550.
Baum
E
E
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002455:Cell Division; D003907:Dexamethasone / Q000494:pharmacology*; D004351:Drug Resistance; D006801:Humans; D007399:Interphase; D007938:Leukemia / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009154:Mutation; D010755:Organophosphates / Q000378:metabolism; D010758:Phosphorus; D014407:Tumor Cells, Cultured
D010755:Organophosphates; D010758:Phosphorus; D003907:Dexamethasone; D000255:Adenosine Triphosphate