Inhibitory effects of isoquinoline-type alkaloids on leukemic cell growth and macromolecule biosynthesis.
A number of benzylisoquinoline alkaloids of plants indigenous to Taiwan were isolated and purified and their chemical structures were determined. These compounds, namely benzyltetrahydroisoquinolines or their N-oxides, aporphines, oxoaporphines and 1-N, N-dimethylaminoethylphenanthrenes or their N-oxides, were studied for their potency in inhibiting precursor incorporation into DNA, RNA and protein. Inhibition of murine leukemic L1210 and human leukemic CCRF-CEM and HL-60 cell growth was also examined. Of the compounds evaluated for cell growth inhibition, (+-)-N-methylcoclaurine(3), (-)-norannuradhapurine HBr(19), oxoglaucine (23), dicentrine methine (27) and 1-(N-methyl-N-hydroxyaminoethyl)-3, 4-methylenedioxy-7-methoxyphenanthrene (28) showed an IC50 less than 10 microM.
Tzeng
C C
CC
School of Pharmacy, Kaohsiung Medical College, Taiwan, Republic of China.
Wu
Y C
YC
Su
T L
TL
Watanabe
K A
KA
Lu
S T
ST
Chou
T C
TC
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic; D002455:Cell Division / Q000187:drug effects; D055598:Chemical Phenomena; D002621:Chemistry; D004273:DNA, Neoplasm / Q000096:biosynthesis; D006801:Humans; D007546:Isoquinolines / Q000494:pharmacology*; D007938:Leukemia / Q000378:metabolism / Q000473:pathology*; D007939:Leukemia L1210 / Q000378:metabolism / Q000473:pathology; D051379:Mice; D009363:Neoplasm Proteins / Q000096:biosynthesis; D012334:RNA, Neoplasm / Q000096:biosynthesis; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology
D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D004273:DNA, Neoplasm; D007546:Isoquinolines; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm