Differences between muscarinic-receptor- and Ca2(+)-induced inositol polyphosphate isomer accumulation in rat cerebral-cortex slices.
Muscarinic-receptor stimulation or depolarization by elevated K+ leads to increased accumulation of [3H]Ins(1,4,5)P3, [3H]Ins(1,3,4,5)P4 and several degradation products of these polyphosphates separated by h.p.l.c. On the other hand, agents such as ionomycin and maitotoxin, which increase intracellular Ca2+ directly, produce a small accumulation of [3H]Ins(1,4,5)P3 and markedly increase [3H]Ins(1,4)P2, but [3H]Ins(1,3,4,5)P4, [3H]Ins(1,3,4)P3 and [3H]Ins(1,3)P2 are virtually unaffected. Ca2(+)-dependent [3H]inositol polyphosphate metabolism may involve different pools of lipids and/or phosphoinositidases.
Baird
J G
JG
Department of Pharmacology and Therapeutics, University of Leicester, U.K.
Nahorski
S R
SR
D000818:Animals; D002118:Calcium / Q000502:physiology*; D002540:Cerebral Cortex / Q000378:metabolism*; D066298:In Vitro Techniques; D007295:Inositol Phosphates / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D011976:Receptors, Muscarinic / Q000502:physiology*
D007295:Inositol Phosphates; D011976:Receptors, Muscarinic; D002118:Calcium