[Study of the receptor for black widow spider neurotoxin. II. Isolation and characteristics of the receptor from bovine brain membranes].
Biospecific sorbents for isolation of the latrotoxin receptor have been obtained and studied. Binding of the receptor components solubilized from the bovine cerebral cortex membrane to the immobilized toxin critically depends on the presence of calcium ions and the solution ionic strength. The procedure for semipreparative isolation of the highly active receptor preparation with Kd = 9 x 10(-10) M and Bmax = 0.9 nmol/mg has been developed. Binding activity of the isolated receptor is inhibited by heating as well as by proteases and denaturing agents. According to electrophoretic analysis in the presence of SDS the receptor complex contains protein components of molecular mass 200, 160, 79, 43 kDa, the former two being glycoproteins.
Petrenko
A G
AG
Surkova
I N
IN
Shamotienko
O G
OG
Kovalenko
V A
VA
Krasnoperov
V N
VN
Tret'iakov
L A
LA
Ushkarev
Iu A
IuA
Grishin
E V
EV
D000818:Animals; D001180:Arthropod Venoms / Q000378:metabolism*; D001923:Brain Chemistry; D002417:Cattle; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008566:Membranes / Q000378:metabolism; D008970:Molecular Weight; D009498:Neurotoxins / Q000378:metabolism*; D011950:Receptors, Cholinergic / Q000302:isolation & purification* / Q000378:metabolism; D018000:Receptors, Peptide; D013111:Spider Venoms / Q000378:metabolism*
D001180:Arthropod Venoms; D009498:Neurotoxins; D011950:Receptors, Cholinergic; D018000:Receptors, Peptide; D013111:Spider Venoms; C034800:alpha-latrotoxin receptor; C020763:alpha-latrotoxin; C038599:black widow spider venom
Issledovanie retseptora neĭrotoksina pauka karakurta. II. Vydelenie i kharakteristika reteseptora iz membran mozga byka.