title: Surface proteins of the gliding bacterium Cytophaga sp. strain U67 and its mutants defective in adhesion and motility.
abstract: Surface proteins of the gliding bacterium Cytophaga sp. strain U67 that make contact with glass substrata were radioiodinated, using a substratum-immobilized catalyst (Iodo-Gen). At least 15 polypeptides were iodinated, fewer than the number labeled by surface biotinylation of whole cells; these polypeptides define the set of possible candidates for the surface protein(s) that mediates gliding-associated substratum adhesion. The labeling of three adhesion-defective mutants exhibited two characteristic patterns of surface iodination which involved addition, loss, or alteration of several polypeptides of high molecular weight. An adhesion-competent revertant of mutant Adh3 and one of Adh2 exhibited the wild-type labeling pattern. Two other Adh2 revertants resembled their adhesion-defective parent. The labeling pattern of surface polypeptides of a nongliding but adhesive cell strain was similar to that of the wild type.
authors.affiliation: Department of Biological Sciences, University of Maryland Baltimore County 21228.
authors.forename: R P
authors.initials: RP
authors.lastname: Burchard
authors.forename: R A
authors.initials: RA
authors.lastname: Bloodgood
mesh_terms: D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000032:analysis*; D001710:Biotin; D003589:Cytophaga / Q000032:analysis*; D007457:Iodine Radioisotopes; D008970:Molecular Weight; D009068:Movement; D009154:Mutation; D012394:Rosaniline Dyes
chemical_list: D001426:Bacterial Proteins; D007457:Iodine Radioisotopes; D012394:Rosaniline Dyes; D001710:Biotin; C048139:Coomassie blue