Simplified solid-phase immunobead assay for detection of ciguatoxin and related polyethers.
This study examined the development of a highly simplified solid-phase colored latex immunobead assay for the detection of ciguatoxin and related polyethers. This procedure was compared with the stick enzyme immunoassay previously reported. Chi-square analysis of two separate experiments on 153 and 283 fish of various species gave chi 2 values of p less than 0.001 and p less than 0.005, respectively. Agreement between the two procedures with 26 fish implicated in ciguatera poisoning was 100%. A preliminary assessment in the field showed encouraging results. The procedure appears to be simple and applicable to field use. Furthermore, this procedure should be applicable to other antibody-antigen detections, especially low dalton determinations.
Hokama
Y
Y
Department of Pathology, John A. Burns School of Medicine, University of Hawaii, Honolulu 96822.
D000818:Animals; D002922:Ciguatoxins / Q000032:analysis*; D005399:Fishes; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008387:Marine Toxins / Q000032:analysis* / Q000302:isolation & purification; D008985:Monensin / Q000302:isolation & purification; D035964:Oxocins; D000095702:Polyether Toxins
D002922:Ciguatoxins; D008387:Marine Toxins; D008985:Monensin; D035964:Oxocins; C053342:brevetoxin; D000095702:Polyether Toxins; C020394:maitotoxin