Complementation of N-hydroxyethyl-N-chloroethylnitrosourea hypersensitivity of Mex- cells by microinjection of mRNA from Mex+ cells.
Chinese hamster ovary (CHO-9) and HeLa MR cells lack detectable amounts of O6-alkylguanine DNA alkyltransferase (phenotypically Mex-) and are hypersensitive to the toxic effect of N-hydroxyethyl-N-chloroethylnitrosourea (HeCNU), as compared to Mex+ derivatives. Microinjection of size-fractionated polyA+ mRNA extracted from HeLa S3 (Mex+) into CHO-9 and HeLa MR cells, or from ataxia telangiectasia (Mex+) into HeLa MR cells, gave rise to an increase in survival following treatment with toxic doses of HeCNU. Transient complementation of the Mex- phenotype was achieved with an RNA population 0.8-1 kb in size.
Fritz
G
G
Kernforschungszentrum Karlsruhe, Institut für Genetik und für Toxikologie von Spaltstoffen, F.R.G.
Rahmsdorf
H J
HJ
Kaina
B
B
D000818:Animals; D006224:Cricetinae; D004587:Electrophoresis, Agar Gel; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008780:Methyltransferases / Q000494:pharmacology*; D008845:Microinjections; D009607:Nitrosourea Compounds / Q000633:toxicity*; D019853:O(6)-Methylguanine-DNA Methyltransferase; D012333:RNA, Messenger / Q000235:genetics
D009607:Nitrosourea Compounds; D012333:RNA, Messenger; D008780:Methyltransferases; D019853:O(6)-Methylguanine-DNA Methyltransferase; C013876:elmustine