Contrary effect of eye enucleation on VIP-immunoreactive neurons in the suprachiasmatic nucleus and the superior colliculus of the rat.
The topographic alternation of vasoactive intestinal peptide (VIP) immunoreactivity after long-term bilateral eye enucleation (80 days) was investigated in the suprachiasmatic nucleus (SCN) and the superior colliculus of the adult rat with the peroxidase-antiperoxidase method. In the SCN, the immunostaining of VIP-immunoreactive (IR) cell bodies increased, and the intensity and density of VIP-IR fibers and terminals were markedly enhanced after eye enucleation. On the other hand, after eye enucleation, no VIP-IR cell bodies and fibers were visible in the stratum griseum superficiale of the superior colliculus, although fusiform-shaped VIP-IR neurons were distributed in the layer of unoperated control rats. The present results, therefore, have revealed that the long-term blockage of retinal input induces changes of VIP immunoreactivity in different manners, according to the target brain areas.
Okamoto
S
S
Department of Ophthalmology, Kyoto Prefectural University of Medicine, Japan.
Okamura
H
H
Takahashi
Y
Y
Akagi
Y
Y
Yanaihara
N
N
Ibata
Y
Y
D000818:Animals; D007150:Immunohistochemistry; D008297:Male; D009799:Ocular Physiological Phenomena; D051381:Rats; D011919:Rats, Inbred Strains; D012683:Sensory Deprivation; D013477:Superior Colliculi / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D013493:Suprachiasmatic Nucleus / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*; D014795:Visual Pathways / Q000033:anatomy & histology / Q000378:metabolism* / Q000502:physiology
D014660:Vasoactive Intestinal Peptide