Crystallization of a soluble form of the rat T-cell surface glycoprotein CD4 complexed with Fab from the W3/25 monoclonal antibody.
The structure of the T-lymphocyte cell surface glycoprotein CD4 is of considerable biological and medical interest. Recombinant rat CD4 expressed in soluble form in mammalian cells and complexed with W3/25 monoclonal Fab fragments formed crystals that diffract to 3.5 A and have the orthorhombic space group P2(1)2(1)2 or P2(1)2(1)2(1). The unit cell has dimensions a = 317 A, b = 161 A and c = 41.8 A and the asymmetric unit consists of two CD4:Fab complexes. These crystals are of suitable quality for X-ray diffraction analysis.
Davis
S J
SJ
MRC Cellular Immunology Research Unit, Sir William Dunn School of Pathology, Oxford, U.K.
Brady
R L
RL
Barclay
A N
AN
Harlos
K
K
Dodson
G G
GG
Williams
A F
AF
D000818:Animals; D000911:Antibodies, Monoclonal; D015704:CD4 Antigens / Q000235:genetics / Q000276:immunology; D003460:Crystallization; D007140:Immunoglobulin Fab Fragments; D051381:Rats; D011994:Recombinant Proteins; D012995:Solubility; D014961:X-Ray Diffraction
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D007140:Immunoglobulin Fab Fragments; D011994:Recombinant Proteins