title: [Inheritance and expression of cholera toxin genes introduced into Vibrio cholerae el tor cells in a hybrid plasmid].
abstract: Two replicons, pOX38 (in F-factor derivative lacking all IS elements) and pCT105 (containing cholera toxin operon cloned in pBR322) have been fused to produce recombinant plasmid, pCO109, which was then introduced into Vibrio cholerae eltor by conjugation. Restriction analysis showed pCO109 to dissociate in V. cholerae cells at a higher frequency than in Escherichia coli strains, its pOX38 component being lost, while the pCT105 component demonstrated relative stability. V. cholerae eltor RV79 (pCT105) produced 4-5 micrograms/ml of cholera toxin. Occasional insertion of cloned vctA, B operon into RV79 chromosome was also observed.
authors.forename: N I
authors.initials: NI
authors.lastname: Smirnova
authors.forename: G A
authors.initials: GA
authors.lastname: Eroshenko
authors.forename: L F
authors.initials: LF
authors.lastname: Livanova
authors.forename: O T
authors.initials: OT
authors.lastname: Mozharov
authors.forename: S L
authors.initials: SL
authors.lastname: Fil'kova
authors.forename: T S
authors.initials: TS
authors.lastname: Il'ina
mesh_terms: D002772:Cholera Toxin / Q000096:biosynthesis / Q000235:genetics*; D003227:Conjugation, Genetic; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D010957:Plasmids; D011995:Recombination, Genetic; D014734:Vibrio cholerae / Q000235:genetics* / Q000378:metabolism
chemical_list: D002772:Cholera Toxin
vernacular_title: Osobennosti nasledovaniia i ékspressii genov kholernogo toksina, vvedennykh v kletki Vibrio cholerae eltor v sostave gibridnoĭ plazmidy.