Partial purification of a boar sperm membrane protein inducing sperm agglutinating antibody.
For the development of immunological contraception, attention is being concentrated on the possibility of using a sperm membrane antigen. Boar sperm membrane was extracted with triton-X 100 and fractionated by Sephadex G-150 column chromatography. The glycosylated and nonglycosylated portions of protein peaks from the gel filtration were obtained by fractionating on concanavalin A-Sepharose and eluting the bound protein with 0.3 M methyl mannoside. A glycosylated fraction was found to induce sperm agglutinating antibodies in rabbit. The partially purified protein has a molecular weight of 30 kilodaltons, as determined by sodium dodecyl polyacrylamide gel electrophoresis. Further work is planned on the histochemical determination of the origin of this protein and species cross-activity of the antibody.
Adeyemo
O
O
Department of Veterinary Anatomy, University of Ibadan, Nigeria.
Okegbile
E O
EO
Olorunsogo
O O
OO
D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D008565:Membrane Proteins / Q000276:immunology / Q000302:isolation & purification*; D013073:Sperm Agglutination; D013094:Spermatozoa / Q000276:immunology*; D013552:Swine
D000941:Antigens; D008565:Membrane Proteins