title: Isolation and characterization of the 5S rRNA gene of Leptospira interrogans.
abstract: The gene encoding the 5S rRNA for Leptospira interrogans serovar canicola strain Moulton was isolated and sequenced. The 5S rRNA gene occurs as a single copy within the genome and encodes a 117-nucleotide-long RNA molecule. The 5S rRNA gene is flanked at both the 5' and 3' ends by regions of A + T-rich sequences, and the 5'-flanking region contains a promoter sequence. L. interrogans has a unique and remarkable organization of the 5S rRNA gene. The 5S rRNA molecule exhibits a strong similarity to typical eubacterial 5S rRNA in terms of overall secondary structure, while the primary sequence is conserved to a lesser degree. Restriction analysis of the 5S rRNA gene indicated that the DNA sequence including the 5S rRNA gene is highly conserved in the genomes of parasitic leptospires.
authors.affiliation: Faculty of Pharmacy and Pharmaceutical Science, University of Fukuyama, Hiroshima, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Fukunaga
authors.forename: I
authors.initials: I
authors.lastname: Horie
authors.forename: I
authors.initials: I
authors.lastname: Mifuchi
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis; D005798:Genes, Bacterial; D007921:Leptospira interrogans / Q000235:genetics*; D008969:Molecular Sequence Data; D012335:RNA, Ribosomal / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D012335:RNA, Ribosomal; D012341:RNA, Ribosomal, 5S