Analysis of a CR1 (chicken repeat) sequence flanking the 5' end of the gene encoding alpha-skeletal actin.
Genomic Southern blots of chicken DNA, probed with radiolabeled DNA fragments that flank the 5'-end of the asa gene encoding chicken alpha-skeletal actin, indicate the presence of repetitive nucleotide sequences. Sequence analysis of this region reveals a member of the CR1 family of middle-repetitive elements. A 186-bp restriction fragment carrying the 3'-end of this CR1 element binds factor(s) present in nuclear extracts, as assayed by band-shift electrophoresis. However, the CR1 repeat has limited influence on transcription from the alpha-skeletal actin promoter, as assessed by CAT assays of transfected chicken myoblasts and fibroblasts.
French
B A
BA
Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030.
Bergsma
D J
DJ
Schwartz
R J
RJ
D000199:Actins / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002645:Chickens / Q000235:genetics*; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D005796:Genes / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D014157:Transcription Factors; D014162:Transfection
D000199:Actins; D004274:DNA, Recombinant; D009687:Nuclear Proteins; D014157:Transcription Factors; D015500:Chloramphenicol O-Acetyltransferase