Cryopreservation of unfertilized mouse oocytes from inbred strains by ultrarapid freezing.
Unfertilized mouse oocytes from inbred strains (BALB/c, C3H/He and C57BL/6) were frozen ultrarapidly by direct plunging into liquid nitrogen, immediately after exposure to a highly-concentrated solution (DAP 213: 2 M dimethyl sulphoxide, 1 M acetamide, and 3 M propylene glycol in PB 1), and were later thawed in a 37 degrees C waterbath. After thawing, 76.8-90.9% of recovered oocytes were morphologically normal. Following fertilization in vitro of cryopreserved oocytes, the proportion of 2-cell embryos 24 h after insemination ranged from 70.7% to 83.4%. Nearly all 2-cell embryos obtained from cryopreserved oocytes were transferred to the oviducts of pseudopregnant recipients and 31.0-43.0% of 2-cell embryos developed into normal young.
Nakagata
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Division of Pathology, Juntendo University School of Medicine, Tokyo, Japan.
D000818:Animals; D015925:Cryopreservation / Q000379:methods; D004624:Embryo Transfer; D005314:Embryonic and Fetal Development; D005260:Female; D005307:Fertilization in Vitro; D005615:Freezing; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009865:Oocytes; D011247:Pregnancy