title: Basal prostaglandin synthesis by the isolated perfused rat kidney.
abstract: In order to assess the main characteristics of the prostaglandin (PG) biosynthesis by the isolated perfused rat kidney, the urinary and venous outputs of PGE2, PGF2alpha, 6-keto-PGF1alpha and of thromboxane (Tx)B2 were followed during 120 min after an equilibration period of 30 min. Single pass kidneys were perfused with a Krebs-Henseleit solution added with Polygeline at a constant flow rate providing a perfusion pressure about 90 mm Hg. From the beginning of the study, major differences could be observed in the renal biosynthetic rate of the 4 PG studied which were mainly excreted into the venous effluent. During the perfusion, urinary and venous outputs of PGE2, PGF2alpha and of TxB2 remained stable whereas those of 6-keto-PGF1alpha sharply increased and were found inversely related to the glomerular filtration rate (r = -0.95; p n 0.001). Finally, the urinary and venous outputs of each of the four PGs studied were found positively related. It is concluded that the isolated perfused rat kidney is a valuable preparation for studying the biosynthesis of PGs and that, at least in thi model, the urinary excretion of PGs is a good index of their renal synthesis.
authors.affiliation: Department of Physiology and Clincal Pharmacology, UA CNRS 606, Faculty of Pharmacy, Lyon, France.
authors.forename: K L
authors.initials: KL
authors.lastname: Liu
authors.forename: A
authors.initials: A
authors.lastname: Hadj Aissa
authors.forename: M C
authors.initials: MC
authors.lastname: Lareal
authors.forename: D
authors.initials: D
authors.lastname: Benzoni
authors.forename: J
authors.initials: J
authors.lastname: Sassard
authors.forename: P
authors.initials: P
authors.lastname: Zech
mesh_terms: D000818:Animals; D015237:Dinoprost / Q000096:biosynthesis / Q000097:blood / Q000652:urine; D015232:Dinoprostone / Q000096:biosynthesis / Q000097:blood / Q000652:urine; D066298:In Vitro Techniques; D007668:Kidney / Q000378:metabolism*; D008297:Male; D010477:Perfusion; D011453:Prostaglandins / Q000096:biosynthesis*; D011460:Prostaglandins F / Q000096:biosynthesis / Q000097:blood / Q000652:urine; D051381:Rats; D011919:Rats, Inbred Strains; D013929:Thromboxane B2 / Q000096:biosynthesis / Q000097:blood / Q000652:urine
chemical_list: D011453:Prostaglandins; D011460:Prostaglandins F; D013929:Thromboxane B2; D015237:Dinoprost; D015232:Dinoprostone; C100006:prostaglandin F1