cDNA cloning and characterization of ret activated in a human papillary thyroid carcinoma cell line.
We obtained activated ret cDNAs (retTPC) from a human papillary thyroid carcinoma cell line, TPC-1, and characterized its structure. The nucleotide sequence indicated that the recombination had occurred just upstream of the kinase domain of ret proto-oncogene and that the position, where the conserved sequence of ret proto-oncogene starts in retTPC transcripts, was exactly the same as that of ret-II which we have previously analyzed. Furthermore, a unique 13-glycine stretch, which is also present in a small subunit of the calcium dependent protease, calpain, was detected in the replaced sequence of retTPC. The aberrant tyrosine kinase activity induced by the rearrangement of ret proto-oncogene could be involved in the development of papillary thyroid carcinoma.
Ishizaka
Y
Y
Carcinogenesis Division, National Cancer Center Research Institute, Tokyo, Japan.
Ushijima
T
T
Sugimura
T
T
Nagao
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D002291:Carcinoma, Papillary / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D006801:Humans; D008969:Molecular Sequence Data; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes; D015183:Restriction Mapping; D013964:Thyroid Neoplasms / Q000235:genetics*; D014407:Tumor Cells, Cultured
C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D004247:DNA