title: Determination of biological peptide leukotrienes C4 and D4 by fluorometric detection combined with high-performance liquid chromatography.
abstract: A fluorometric procedure combined with high-performance liquid chromatography (HPLC) has been developed to separate and quantitate peptide-leukotrienes (p-LTs) C4 and D4. The method is based on pre-column dansylation of the amino terminus of the p-LTs. Its application for the determination of the amounts of p-LTs C4 and D4 in human blood and sputum is also described. The lower limit of detection of p-LTs with this method is 1 pmol.
authors.affiliation: Institute of Clinical Medicine, University of Tsukuba, Ibaraki, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yamashita
authors.forename: Y
authors.initials: Y
authors.lastname: Uchida
authors.forename: A
authors.initials: A
authors.lastname: Nomura
authors.forename: S
authors.initials: S
authors.lastname: Hasegawa
authors.forename: S
authors.initials: S
authors.lastname: Kimura
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003619:Dansyl Compounds; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D011863:Radioimmunoassay; D013189:SRS-A / Q000032:analysis* / Q000097:blood; D013183:Sputum / Q000032:analysis
chemical_list: D003619:Dansyl Compounds; D013189:SRS-A