title: Placental CSF-like activity.
abstract: We have examined amniotic fluid and cultured supernatants of a variety of placental cells for the presence of colony-stimulating factor (CSF) activity. The supernatants of cultured human choriocarcinoma cell lines, Jeg and Jar, contained CSF activity that induced the formation of granulocyte-macrophage colony-forming unit (CFU-GM) and multipotential colony-forming unit (CFU-GEMM) colonies. Cultured supernatants of nontransformed rat placental cells, chorionic villous cells, and amniotic fluid also induced CFU-GM colonies.
authors.affiliation: Department of Developmental Hematology, Cleveland Clinic Foundation.
authors.forename: R
authors.initials: R
authors.lastname: Meagher
authors.forename: A
authors.initials: A
authors.lastname: Amsden
authors.forename: J
authors.initials: J
authors.lastname: Hunt
authors.forename: M
authors.initials: M
authors.lastname: Soares
authors.forename: L
authors.initials: L
authors.lastname: Sogor
authors.forename: R N
authors.initials: RN
authors.lastname: Smith
mesh_terms: D000653:Amniotic Fluid / Q000032:analysis; D000818:Animals; D002822:Choriocarcinoma / Q000378:metabolism; D002824:Chorionic Villi / Q000378:metabolism; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D003470:Culture Media / Q000032:analysis; D004912:Erythrocytes; D005260:Female; D006098:Granulocytes; D006801:Humans; D008264:Macrophages; D008533:Megakaryocytes; D051379:Mice; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D014327:Trophoblasts / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms / Q000378:metabolism
chemical_list: D003115:Colony-Stimulating Factors; D003470:Culture Media