title: Leukoagglutinins in patients with hemophilia.
abstract: Anti-leukocyte antibodies may occur in hemophilic patients as a consequence of replacement therapy with blood derivates. In this report we describe the presence of leukoagglutinins (LA) in serum of HIV-infected hemophiliacs (HIV + He) and their absence in HIV-negative patients (HIV-He). LA activity was recovered in IgG fractions from HIV + He, in the polyethylene glycol (PEG) precipitates from these sera, and in some cases in the supernatant fractions of PEG precipitates. Although the amount of PEG precipitates corresponding to circulating immune complexes (CIC) was higher in HIV + He than in HIV-He and normals, there was no direct relationship between CIC levels and LA. LA reacted both with autologous and with allogeneic polymorphonuclear leukocytes (PMN). In contrast to PMN isolated from HIV-He, HIV + He PMN had membrane associated IgG. In HIV + He, LA activity was more frequently observed in patients with more advanced stages of HIV infection than in asymptomatic individuals. Our results suggest that LA activity could be one of the manifestations of autoreactivity associated with progressing HIV infection in patients with hemophilia.
authors.affiliation: Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina, Buenos Aires, Argentina.
authors.forename: N E
authors.initials: NE
authors.lastname: Riera
authors.forename: N
authors.initials: N
authors.lastname: Galassi
authors.forename: A
authors.initials: A
authors.lastname: Anselmo
authors.forename: M
authors.initials: M
authors.lastname: Aixala
authors.forename: M B
authors.initials: MB
authors.lastname: Bouza
authors.forename: M M
authors.initials: MM
authors.lastname: de Bracco
mesh_terms: D000293:Adolescent; D000328:Adult; D000936:Antigen-Antibody Complex / Q000032:analysis; D001323:Autoantibodies / Q000096:biosynthesis*; D015551:Autoimmunity; D002648:Child; D006679:HIV Seropositivity / Q000150:complications / Q000276:immunology*; D006467:Hemophilia A / Q000150:complications / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000276:immunology; D007962:Leukocytes / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology
chemical_list: D000936:Antigen-Antibody Complex; D001323:Autoantibodies; D007074:Immunoglobulin G