title: Influence of acute metabolic acidosis on the monomer and polymer forms of renal phosphate-dependent glutaminase.
abstract: Phosphate-dependent glutaminase (PDG) was measured in kidney cortex homogenates and mitochondria from control and acutely acidotic rats. The effect of plasma from acutely acidotic rats on PDG activity in homogenates from normal rats was also studied. Acidosis or incubation in acidotic plasma enhanced enzyme activity when measured at 1.0 mM but not at 20.0 mM glutamine. This effect was not due to increased mitochondrial permeability since similar results were obtained after solubilization of the enzyme with Triton X-100. Increased enzyme activity was observed with either the Tris (monomer) form or the borate (polymer) form of the enzyme, indicating that enhanced activity is not due to polymerization but probably to a conformational change in the enzyme such that the Km for glutamine is lowered.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Granada, España.
authors.forename: P
authors.initials: P
authors.lastname: Hortelano
authors.forename: L
authors.initials: L
authors.lastname: García-Salguero
authors.forename: J A
authors.initials: JA
authors.lastname: Lupiáñez
authors.forename: G A
authors.initials: GA
authors.lastname: Alleyne
mesh_terms: D000138:Acidosis / Q000201:enzymology*; D000208:Acute Disease; D000818:Animals; D004789:Enzyme Activation; D005972:Glutaminase / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism; D007672:Kidney Cortex / Q000201:enzymology*; D007700:Kinetics; D008297:Male; D008928:Mitochondria / Q000201:enzymology*; D011108:Polymers; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D011108:Polymers; D005973:Glutamine; D005972:Glutaminase