Purification and characterization of inhibitor against the cysteine proteinase of Rana catesbeiana.
1. Inhibitors of cysteine proteinase were found in tadpole tail of metamorphosing bullfrog. 2. One of the inhibitors was purified by affinity chromatography with CM-papain agarose, gel filtration with Superose 12 and ion exchange chromatography with Mono S. 3. The molecular weight of the inhibitor was 130,000-140,000 and the isoelectric point was pH 9.6. 4. The inhibitor had inhibitory effects on ficin, papain and tadpole tail cysteine proteinase. 5. The inhibitor is possibly involved in the regulation of muscle degradation in tail regression of metamorphosing tadpole.
Fujita
S
S
Life Science Institute, Sophia University, Tokyo, Japan.
Horiuchi
S
S
D000818:Animals; D002845:Chromatography; D015853:Cysteine Proteinase Inhibitors; D007526:Isoelectric Point; D007814:Larva / Q000378:metabolism; D008970:Molecular Weight; D011480:Protease Inhibitors / Q000302:isolation & purification*; D011892:Rana catesbeiana / Q000378:metabolism*
D015853:Cysteine Proteinase Inhibitors; D011480:Protease Inhibitors