Detection and identification of HLA antigens in dried spleens by means of enzyme linked immunosorbent assay (ELISA).
HLA class-I and class-II antigens were detected in extracts of dry spleen tissue allowed to age from 1 to 17 months, using monomorphic anti HLA monoclonal antibodies and the ELISA technique. Using polymorphic HLA alloantisera samples of spleen tissue could be characterized and differentiated. The potential use of dried tissues and blood stains is discussed.
Bishara
A
A
Department of Clinical Microbiology, Hadassah University Hospital, Jerusalem, Israel.
Brautbar
C
C
D003890:Desiccation; D004797:Enzyme-Linked Immunosorbent Assay; D006680:HLA Antigens / Q000032:analysis*; D006801:Humans; D009926:Organ Preservation; D013154:Spleen / Q000276:immunology*
D006680:HLA Antigens