Determination of tebufelone, a new anti-inflammatory drug, in plasma and tissue using capillary gas chromatography/stable isotope dilution mass spectrometry.
A capillary gas chromatographic/mass spectrometric method for the determination of tebufelone, a new anti-inflammatory drug, in plasma and rat paw tissue is described. Trideuteriated tubufelone was employed as an internal standard and the drug quantified by selected ion monitoring using a mass-selective detector following positive electron ionization (70 eV). Tebufelone was recovered from plasma (greater than 93%) and pulverized paw tissue (greater than 88%) by ethereal extraction. Standard curves were linear between 5 ng ml-1 and 5 micrograms ml-1 with a detection limit (S/N = 2) of 2 ng ml-1. The coefficient of variation for successive injections of standards (200 ng ml-1) or samples (10-1500 ng ml-1) was 7.3% and 8.5%, respectively.
Eichhold
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Procter & Gamble Co., Miami Valley Laboratories, Cincinnati, Ohio 45239-8708.
Doyle
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MJ
D000480:Alkynes / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D008401:Gas Chromatography-Mass Spectrometry; D066298:In Vitro Techniques; D010636:Phenols / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D051381:Rats; D014018:Tissue Distribution
D000480:Alkynes; D000894:Anti-Inflammatory Agents, Non-Steroidal; D010636:Phenols; C064268:tebufelone