title: Transformation of the rice blast fungus Magnaporthe grisea to hygromycin B resistance.
abstract: Low frequency, integrative transformation of three fertile hermaphroditic strains of Magnaporthe grisea has been achieved using plasmid pAN7-1 and cosmid pAN7-2, which contain an Escherichia coli hygromycin B phosphotransferase gene linked to Aspergillus nidulans regulatory sequences.
authors.affiliation: Department of Plant Pathology, University of Wisconsin, Madison 53706.
authors.forename: H
authors.initials: H
authors.lastname: Leung
authors.forename: U
authors.initials: U
authors.lastname: Lehtinen
authors.forename: R
authors.initials: R
authors.lastname: Karjalainen
authors.forename: D
authors.initials: D
authors.lastname: Skinner
authors.forename: P
authors.initials: P
authors.lastname: Tooley
authors.forename: S
authors.initials: S
authors.lastname: Leong
authors.forename: A
authors.initials: A
authors.lastname: Ellingboe
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001203:Ascomycota / Q000187:drug effects / Q000235:genetics*; D015139:Blotting, Southern; D003360:Cosmids; D004352:Drug Resistance, Microbial / Q000235:genetics; D006921:Hygromycin B / Q000494:pharmacology*; D010957:Plasmids; D014170:Transformation, Genetic
chemical_list: D000900:Anti-Bacterial Agents; D006921:Hygromycin B