Problems with the use of whole blood as a sample material in novel direct glucose analysers.
We report a marked shift in whole-blood glucose measurements when a widely available glucose analyser is used. We compared a conventional glucose dehydrogenase (GDHG) method after protein precipitation and a direct polarographic method in conjunction with immobilized glucose oxidase. The polarographic method gave mean whole blood glucose levels from 79% to 95% of those measured by the GDHG method. No such difference was found in either plasma or serum samples or in water-based control samples. The stability of glucose in fluoride oxalate tubes appears to be poorer than is usually assumed. We observed a 5-10% decrease in whole-blood glucose concentrations during the first hour after sampling under routine conditions.
Savolainen
K
K
Department of Clinical Chemistry, Kuopio University Central Hospital, Finland.
Viitala
A
A
Puhakainen
E
E
Väisänen
M
M
D001786:Blood Glucose / Q000032:analysis*; D001793:Blood Preservation; D004800:Enzymes, Immobilized; D005188:False Negative Reactions; D042843:Glucose 1-Dehydrogenase; D005948:Glucose Dehydrogenases; D005949:Glucose Oxidase; D006801:Humans; D010070:Oxalates; D011048:Polarography; D012969:Sodium Fluoride
D001786:Blood Glucose; D004800:Enzymes, Immobilized; D010070:Oxalates; D012969:Sodium Fluoride; D005948:Glucose Dehydrogenases; D042843:Glucose 1-Dehydrogenase; D005949:Glucose Oxidase