Induction of apolipoprotein E gene expression in human and experimental atherosclerotic lesions.
Hypercholesterolemia and atherosclerosis were induced in New Zealand White rabbits by cholesterol feeding. Apolipoprotein E mRNA levels in livers were found to be slightly increased, as determined by Northern blots. Apolipoprotein E gene expression was dramatically induced in rabbit atherosclerotic aortas with respect to healthy aortas. However, apolipoprotein E mRNA levels in atherosclerotic aortas were low as compared with the hepatic mRNA levels of the same animals. Interestingly, we also found a significant increase in apolipoprotein E expression in human atheromata with respect to healthy aorta from the same individual. This is the first report on apo E gene induction in human atherosclerotic lesions.
Crespo
P
P
Departamento de Biología Molecular, Facultad de Medicina, Universidad de Cantabria, Santander, Spain.
González
C
C
Ordovás
J M
JM
Ortiz
J M
JM
Rodriguez
J C
JC
León
J
J
D000818:Animals; D001011:Aorta / Q000378:metabolism / Q000473:pathology; D001057:Apolipoproteins E / Q000096:biosynthesis / Q000235:genetics*; D001161:Arteriosclerosis / Q000235:genetics* / Q000473:pathology; D002791:Cholesterol, Dietary / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008099:Liver / Q000378:metabolism*; D011817:Rabbits
D001057:Apolipoproteins E; D002791:Cholesterol, Dietary