[The effect of pH on in vitro deproteinization in orthomyxo- and paramyxoviruses].
Deproteinization effect of nonionic detergent NP-40 on orthomyxoviruses and paramyxoviruses depends on the ionic strength and pH of the medium. Solubilization of the M1 protein is considerably more efficient at pH 6.0-5.0 than at pH 6.5-9.0 for influenza types A, B, C viruses. In contrast, the extraction of matrix protein M from the virions of paramyxoviruses is increased at pH about 9.0. The phenomenon of pH-dependent solubilization of the virions matrix protein is analyzed in connection with the mechanisms of viral invasion of the target cell. Polypeptide M1 is found to be more tightly bound with the nucleocapside of influenza virus with the cleaved hemagglutinine HA1/2 than with the nucleocapside of the virus with the intact HA0.
Zhirnov
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OP
D003902:Detergents / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D017830:Octoxynol; D009975:Orthomyxoviridae / Q000378:metabolism*; D010252:Paramyxoviridae / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D011092:Polyethylene Glycols / Q000494:pharmacology; D014759:Viral Envelope Proteins / Q000378:metabolism*
D003902:Detergents; D010455:Peptides; D014759:Viral Envelope Proteins; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40
Vliianie pH na deproteinizatsiiu in vitro u ortomikso- i paramiksovirusov.