Depolarization of mouse myeloma cell membranes during photodynamic action.
The effect of photodynamic action on plasma membranes was examined using a fluorescent potentiometric indicator [di-SBA-C2(3)] to measure alterations in the plasma membrane potential of mouse myeloma cells treated with zinc phthalocyanine sulfonate and light. Plasma membrane depolarization was observed to be an early event in photodynamic action, showing both photosensitizer concentration and light dose dependence. Depolarization occurred while membrane integrity was retained and appears to be an early event preceding cell death.
Specht
K G
KG
Center for Photochemical Sciences, Bowling Green State University, OH 43403.
Rodgers
M A
MA
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D007211:Indoles / Q000494:pharmacology*; D008027:Light; D008564:Membrane Potentials / Q000187:drug effects / Q000528:radiation effects; D051379:Mice; D009101:Multiple Myeloma; D009942:Organometallic Compounds / Q000494:pharmacology*; D011199:Potentiometry; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D015032:Zinc / Q000494:pharmacology*
D007211:Indoles; D009942:Organometallic Compounds; C064328:zinc phthalocyanine sulfonate; C064329:zinc phthalocyanine disulfonate; D015032:Zinc