Comparative distribution of marrow CFU-e and CFU-gm progenitors in different anatomic sites in the dog.
The interpretation of marrow cloning activity, particularly in serial cultures, is greatly influenced by the reproducibility of the collected marrow samples. In order to establish whether bone marrow cloning activities and precision of the cloning assays are influenced by the site of bone marrow collection in the dog, we studied the incidence of marrow erythroid (CFU-e) and granulocyte-macrophage (CFU-gm) progenitor cells in the iliac crest, sternum, vertebrae, femur, and humerus, using microplasma clot and soft agar culture systems. Marrow samples obtained from the femur and humerus revealed consistently higher cell concentrations than those from the iliac crest, vertebrae, or sternum. Those aspirated from the sternum and vertebrae had lower cell concentrations and were less reproducible. Statistical analysis revealed no significant differences in the incidence of marrow CFU-e and CFU-gm progenitor cells between the femur, humerus, iliac crest or vertebrae. With multiple sampling, the marrow cloning efficiency was consistent and reproducible within the individual dogs. We conclude that the distribution of CFU-e and CFU-gm is comparable throughout the active marrow in the dog and that these sites may be used interchangeably for multiple quantitative analysis of marrow hematopoietic progenitor cells.
Deldar
A
A
Toxicology Division, Lilly Research Laboratories, Eli Lilly and Co., Greenfield, Indiana 46140.
Stevens
C E
CE
Beineke
P J
PJ
D000362:Agar; D000818:Animals; D001853:Bone Marrow / Q000033:anatomy & histology; D001854:Bone Marrow Cells; D001856:Bone Marrow Examination; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay / Q000379:methods; D003115:Colony-Stimulating Factors / Q000302:isolation & purification; D003470:Culture Media; D004285:Dogs; D004912:Erythrocytes / Q000502:physiology; D005260:Female; D006098:Granulocytes / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D008264:Macrophages / Q000502:physiology; D008297:Male; D010949:Plasma; D013154:Spleen / Q000166:cytology
D003115:Colony-Stimulating Factors; D003470:Culture Media; D000362:Agar