Determination of the enantiomers of suprofen and [2H3]suprofen in plasma by capillary gas chromatography-mass spectrometry.
A method for the stereoselective assay of the (+)- and (-)-enantiomers of suprofen and [2H3]suprofen in human plasma was developed using gas chromatography-mass spectrometry-selected-ion monitoring. (+/-)-[2H7]Suprofen was used as an internal standard. The method involved diethyl ether extraction and chiral derivatization with S-(-)-1-(naphthyl)ethylamine to form diastereomeric amide. The diastereoisomers were separated on a capillary gas chromatograph-mass spectrometer. Quantitation was achieved by selected-ion monitoring of the quasi-molecular ions of the diastereoisomers. The sensitivity, specificity, accuracy and reproducibility of the method were demonstrated to be satisfactory for application to pharmacokinetic studies of suprofen enantiomers.
Shinohara
Y
Y
Tokyo College of Pharmacy, Japan.
Kirii
N
N
Tamaoki
H
H
Magara
H
H
Baba
S
S
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D013237:Stereoisomerism; D013496:Suprofen / Q000097:blood*
D013496:Suprofen