title: The consequences of asanguineous versus sanguineous reperfusion after long-term preservation of the heart.
abstract: We have used the heterotopically transplanted rat heart to investigate whether the nature (sanguineous or asanguineous) of the initial period of reperfusion after hypothermic cardioplegic storage influences the postischaemic recovery of the heart. Excised rat hearts were arrested by infusion (1 min at 25 degrees C followed by 2 min at 7.5 degrees C) with the St Thomas' Hospital cardioplegic solution, subjected to 4 h of storage at 7.5 degrees C and heterotopically transplanted over a fixed period of 45 min. Reperfusion was then carried out for 80 min according to one of the following protocols: 60 or 20 min of blood perfusion in situ followed by excision, and 20 or 60 min of in vitro perfusion with crystalloid solution (Groups I and II, respectively) or immediate excision and 80 min of crystalloid perfusion (Group III). Intraventricular balloons were used to define pressure-volume relationships at the end of the 80 min period of reperfusion. Tissue samples were then taken for assessment of water content, adenosine triphosphate (ATP) and creatine phosphate (CP) content. Mean left ventricular developed pressure (at a loading volume of 110 microliters) was 92 +/- 6, 79 +/- 6 and 51 +/- 6 mmHg in Groups I, II and III, respectively. Left ventricular end-diastolic pressure was lower in the initial blood reperfusion groups (25 +/- 4 and 21 +/- 3 mmHg in Group I and II, respectively, compared with 37 +/- 5 mmHg in Group III).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Cardiovascular Research, Rayne Institute, St. Thomas' Hospital, London, UK.
authors.forename: M
authors.initials: M
authors.lastname: Galinanes
authors.forename: D J
authors.initials: DJ
authors.lastname: Hearse
mesh_terms: D000818:Animals; D001769:Blood; D006321:Heart / Q000502:physiology*; D006334:Heart Function Tests; D016027:Heart Transplantation; D009200:Myocardial Contraction; D015425:Myocardial Reperfusion / Q000379:methods*; D015428:Myocardial Reperfusion Injury / Q000209:etiology; D009206:Myocardium / Q000378:metabolism; D009926:Organ Preservation; D051381:Rats; D011917:Rats, Inbred Lew; D012996:Solutions
chemical_list: D012996:Solutions