Effect of the secretions from the IUD-bearing uterus on peri-implantation mouse embryos.
This study was performed to evaluate the effect of IUD-bearing uterine secretions on peri-implantation mouse embryo. 83% of blastocysts degenerated by 96 hr after co-culture with uterine fluids from the IUD-bearing uterus. This degeneration rate was contrasted to 78% after heat treatment (56 degrees C for 30 min) of the IUD-bearing uterine secretions. These results suggest that embryotoxic agents are present in the fluid of the IUD-bearing uterus, especially in the supernatant component and that these agents may be related to the presence of macrophage or a chemical mediator produced by macrophage.
Takeuchi
K
K
Dept. of Obstet. & Gynec., Faculty of Medicine, Kagoshima Univ., Japan.
Mori
A
A
Yamamoto
S
S
Sonoda
T
T
Nagata
Y
Y
D000818:Animals; D001755:Blastocyst / Q000187:drug effects*; D015237:Dinoprost / Q000096:biosynthesis; D015232:Dinoprostone / Q000096:biosynthesis; D047108:Embryonic Development; D005260:Female; D006358:Hot Temperature / Q000009:adverse effects; D007434:Intrauterine Devices; D007958:Leukocyte Count; D051379:Mice; D008813:Mice, Inbred ICR; D009924:Organ Culture Techniques; D011247:Pregnancy; D014327:Trophoblasts / Q000187:drug effects; D014599:Uterus / Q000378:metabolism*
D015237:Dinoprost; D015232:Dinoprostone