Regional and subcellular distribution of cytochrome P-450-dependent drug metabolism in monkey brain: the olfactory bulb and the mitochondrial fraction have high levels of activity.
In brain of female monkey (M. fascicularis) the content of cytochrome P-450 and benzo(a)pyrene hydroxylase activity in the mitochondrial fraction exceeded that of the microsomes by more than 4-fold. The mitochondrial drug metabolism activity exhibited substrate specificity and, unlike the microsomes, did not catalyze 7-ethoxyresorufin O-deethylase reaction. Moreover, the rate of benzo(a)pyrene hydroxylase activity by both the mitochondrial and the microsomal fractions displayed regional variation with the olfactory bulb displaying the highest hydroxylase activity. In contrast, the microsomal 7-ethoxyresorufin O-deethylase activity was uniformally distributed in all brain regions.
Iscan
M
M
Department of Biophysics and Environmental Health Sciences Center, University of Rochester, NY 14642.
Reuhl
K
K
Weiss
B
B
Maines
M D
MD
D000818:Animals; D001579:Benzopyrene Hydroxylase / Q000378:metabolism; D001921:Brain / Q000378:metabolism* / Q000648:ultrastructure; D002451:Cell Compartmentation; D019363:Cytochrome P-450 CYP1A1; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D008252:Macaca fascicularis; D008861:Microsomes / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D009830:Olfactory Bulb / Q000201:enzymology*; D010088:Oxidoreductases / Q000378:metabolism
D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D010088:Oxidoreductases; D001579:Benzopyrene Hydroxylase; D019363:Cytochrome P-450 CYP1A1