A method for counting leukocytes in filtered components.
Leukocyte-depleted components are being used more frequently. Quantitation of residual WRCs is needed for quality control and for establishing the critical level required to prevent alloimmunization. Electronic counting is not accurate at WBC levels below 100/microL (1) and previously proposed techniques are either tedious or require sophisticated equipment. We describe a simple hemacytometer procedure using Türks solution that gives 87-93% recovery at WBC levels below 40/microL.
Greenwalt
T J
TJ
Hoxworth Blood Center, University of Cincinnati Medical Center, OH.
Allen
C M
CM
D005374:Filtration / Q000295:instrumentation; D006801:Humans; D007958:Leukocyte Count / Q000379:methods*