Evidence for a factor protective against hypoxic liver parenchymal cell injury in a protein-free blood extract.
The effect of a protein-free extract from calf blood on hypoxic cell injury was studied in experiments with primary cultures of isolated hepatocytes. At concentrations above 1 mg/ml the extract produced marked protection. At 4 h of anaerobic incubation at 37 degrees C only 20% of cells were damaged as compared to 50% of the anaerobic control incubation. These data demonstrate the presence of a factor in the blood extract which protects against hypoxic cell injury.
de Groot
H
H
Klinische Forschergruppe Leberschädigung, Institut für Physiologische Chemie I, Heinrich-Heine-Universität Düsseldorf, West Germany.
Brecht
M
M
Machicao
F
F
D000818:Animals; D015687:Cell Hypoxia / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006418:Heme / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014343:Trypan Blue
D006418:Heme; C015646:Actovegin; D007770:L-Lactate Dehydrogenase; D014343:Trypan Blue