title: Cytosolic calcium in T lymphocytes from the spontaneously hypertensive rat.
abstract: To gain further insight into the possible generalized nature of intracellular calcium-related alterations in hypertension we measured free cytosolic calcium (iCa2+) in lymphocytes obtained from the spleen and the blood of the spontaneously hypertensive rat (SHR) and age-matched normotensive Wistar-Kyoto (WKY) rats. By monitoring Fura-2 fluorescence at an excitation wavelength of 340 nm and an emission wavelength of 510 nm we estimated; Ca2+. In peripheral lymphocytes from the SHR iCa2+ was increased as compared to those from WKY rats (146 +/- 14 and 112 +/- 6.0 nm, respectively, P less than .025). In contrast, in lymphocytes obtained from the spleen, iCa2+ was not different between SHR and WKY rats (93 +/- 7.0 and 96 +/- 8.0 nm, respectively). When spleen cells were subjected to selective B lymphocyte depletion to generate a preparation of predominantly T lymphocytes, iCa2+ was found to be higher in SHR than in WKY rats (149 +/- 23 and 89 +/- 12 nm, respectively, P less than .05). The finding that iCa2+ is elevated in peripheral lymphocytes and spleen cells subjected to B cell depletion but not in untreated spleen cells indicates that T, but not B, lymphocytes have increased iCa2+ in the SHR. This observation may be related to existing evidence that T lymphocyte function is impaired in the SHR. An increase in iCa2+ is not a phenomenon generalized to all cells of this model of genetic hypertension.
authors.affiliation: Department of Medicine, Northwestern University Medical School, Chicago, Illinois 60611.
authors.forename: D C
authors.initials: DC
authors.lastname: Batlle
authors.forename: G
authors.initials: G
authors.lastname: Janss
authors.forename: M
authors.initials: M
authors.lastname: LaPointe
authors.forename: J
authors.initials: J
authors.lastname: Llibre
authors.forename: A
authors.initials: A
authors.lastname: Saleh
mesh_terms: D000818:Animals; D001572:Benzofurans; D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism*; D016257:Fura-2; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012044:Regression Analysis; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D001572:Benzofurans; D002118:Calcium; D016257:Fura-2