[A new strategy of neonatal screening for cystic fibrosis. The association of immunoreactive trypsin and molecular biology in dried blood].
Cystic fibrosis (CF) screening by means of immunoreactive trypsin (IRT) lacks specificity: only 1 out of 12 hypertrypsinemic neonates has cystic fibrosis. We propose here to analyse the KM.19 polymorphic site in the dried blood spots as an additional test in hypertrypsinemic neonates. A blind retrospective study of 114 hypertrypsinemic samples has been performed after polymerase chain reaction. Twenty-seven of 37 CF (74%) were homozygous for allele 2 (2-2) and could have been diagnosed on the 15th day of life. Fifty-five percent of the infants tested were homozygous for allele 1 (1-1), a very rare feature in CF, conferring them a probability of being normal of 99.8%. At the moment, this test could be of great help in the CF screening, even better than the search for the delta F508 mutation for which 45.9% of CF patients are homozygous.
Laroche
D
D
Service de Biophysique Médicale, CHRU de Caen.
Peres
O
O
Briard
M L
ML
Lemonnier
F
F
Pasquet-Ferre
C
C
Blandin
C
C
Travert
G
G
Fernandez
Y
Y
D003550:Cystic Fibrosis / Q000097:blood / Q000235:genetics / Q000517:prevention & control*; D006720:Homozygote; D006801:Humans; D007231:Infant, Newborn; D008967:Molecular Biology; D015997:Neonatal Screening / Q000639:trends*; D016133:Polymerase Chain Reaction; D014357:Trypsin / Q000097:blood*
D014357:Trypsin
Vers une nouvelle stratégie de dépistage néonatal de la mucoviscidose. Association du dosage de la trypsine immunoréactive et de la biologie moléculaire dans le sang séché.