Clindamycin effect on glycocalyx production in experimental viridans streptococcal endocarditis.
Abundant glycocalyx production by viridans streptococci in the rabbit model of endocarditis has been associated with delayed antimicrobial sterilization. Enzymatic digestion of the glycocalyx with dextranase enhances antibiotic activity. The effect of clindamycin (30 mg/kg, subcutaneous, three times daily) was studied in rabbits with experimental aortic valve endocarditis caused by high glycocalyx-producing viridans streptococci. Animals receiving clindamycin had smaller vegetations that were sterilized more quickly than did controls or animals receiving penicillin or dextranase alone (P less than .001). Penicillin plus dextranase treatment allowed greater bacterial killing than penicillin alone and did not differ significantly from clindamycin treatment. Electron micrographs revealed markedly less cell-adherent glycocalyx on organisms grown in vitro treated with clindamycin versus penicillin and controls. It is hypothesized that clindamycin inhibits glycocalyx production in vivo, allowing better antimicrobial penetration in the infected cardiac vegetation.
Dall
L
L
Department of Medicine, University of Missouri-Kansas City, School of Medicine 64108.
Keilhofner
M
M
Herndon
B
B
Barnes
W
W
Lane
J
J
D000818:Animals; D002981:Clindamycin / Q000494:pharmacology* / Q000627:therapeutic use; D003910:Dextranase / Q000494:pharmacology / Q000627:therapeutic use; D004359:Drug Therapy, Combination; D004697:Endocarditis, Bacterial / Q000188:drug therapy* / Q000382:microbiology; D005260:Female; D006023:Glycoproteins / Q000096:biosynthesis*; D008854:Microscopy, Electron; D010406:Penicillins / Q000494:pharmacology / Q000627:therapeutic use; D011134:Polysaccharides / Q000096:biosynthesis*; D011817:Rabbits; D013290:Streptococcal Infections / Q000188:drug therapy* / Q000382:microbiology; D013291:Streptococcus / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure
D006023:Glycoproteins; D010406:Penicillins; D011134:Polysaccharides; D002981:Clindamycin; D003910:Dextranase