Persistence of added retinoids in organ culture media during induction of mucous metaplasia and glandular morphogenesis in hamster cheek pouches.
The retinoid concentration (determined colorimetrically) did not change significantly in retinyl acetate-supplemented (6 micrograms/ml) Eagle's Minimal Essential Medium containing 10% fetal calf serum when stored at -20 or 4 degrees C over 7 days. After the medium was incubated at 37 degrees C for 48 h, 37-49% of the retinoid remained, whether or not tissue (neonatal Syrian hamster cheek pouch) was present, and irrespective of explant age. The normal retinoid level in the tissue was approximately 0.25 micrograms per gram. Therefore, neonatal hamster cheek pouches, incubated in medium with the addition of 6 micrograms of retinyl acetate per ml of medium and undergoing mucous metaplasia and some mucous gland morphogenesis, were continually being exposed to retinoid levels which, though gradually decreasing, remained well above their normal physiological level.
Robinson
M E
ME
Department of Biomedical Sciences, University of Guelph, Ontario, Canada.
Gibbins
A M
AM
Hardy
M H
MH
D000818:Animals; D000831:Animals, Newborn; D002610:Cheek / Q000473:pathology; D003124:Colorimetry; D006224:Cricetinae; D003470:Culture Media; D004224:Diterpenes; D005088:Exocrine Glands / Q000187:drug effects / Q000254:growth & development*; D008647:Mesocricetus; D008679:Metaplasia; D009024:Morphogenesis / Q000187:drug effects; D009061:Mouth Mucosa / Q000187:drug effects / Q000473:pathology*; D009092:Mucous Membrane / Q000187:drug effects / Q000473:pathology; D009924:Organ Culture Techniques; D012176:Retinoids / Q000378:metabolism*; D000084562:Retinyl Esters; D014801:Vitamin A / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology
D003470:Culture Media; D004224:Diterpenes; D012176:Retinoids; D000084562:Retinyl Esters; D014801:Vitamin A; C009166:retinol acetate