Cytogenetic analyses of mice exposed to dichloromethane.
Chromosome damage was studied in female B6C3F1 mice exposed to dichloromethane (DCM) by subcutaneous or inhalation treatments. No increase in the frequency of either sister chromatid exchanges (SCEs) or chromosome aberrations (CAs) in bone marrow cells was observed after a single subcutaneous injection of 2,500 or 5,000 mg/kg DCM. Inhalation exposure to DCM for 10 days at concentrations of 4,000 or 8,000 ppm resulted in significant increases in frequencies of SCEs in lung cells and peripheral blood lymphocytes, CAs in lung and bone marrow cells, and micronuclei (MN) in peripheral blood erythrocytes. Lung cell CAs and blood erythrocyte MN reached frequencies of approximately two times control levels. Following a 3-month inhalation exposure to 2,000 ppm DCM, mice showed small but significant increases in lung cell SCEs and peripheral blood erythrocyte MN. These findings suggest that genotoxicity may play a role in the carcinogenicity of DCM in the lungs of B6C3F1 female mice.
Allen
J
J
Genetic Toxicology Division, U.S. Environmental Protection Agency, Research Research Triangle Park, NC 27711.
Kligerman
A
A
Campbell
J
J
Westbrook-Collins
B
B
Erexson
G
G
Kari
F
F
Zeiger
E
E
D000280:Administration, Inhalation; D000818:Animals; D001854:Bone Marrow Cells; D002455:Cell Division / Q000187:drug effects; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects*; D005260:Female; D006843:Hydrocarbons, Chlorinated / Q000633:toxicity*; D007279:Injections, Subcutaneous; D007700:Kinetics; D008168:Lung / Q000166:cytology / Q000187:drug effects*; D008752:Methylene Chloride / Q000008:administration & dosage / Q000633:toxicity*; D051379:Mice; D015162:Micronucleus Tests; D012854:Sister Chromatid Exchange / Q000187:drug effects
D006843:Hydrocarbons, Chlorinated; D008752:Methylene Chloride