[Modification of H-phosphonate method of oligonucleotide synthesis on polymer support].
The mechanism of the internucleotide condensation and side-reactions in H-phosphonate approach has been investigated with the help of NMR-spectroscopy. On the basis of the results obtained a modification minimizing side-reactions in the course of the nucleotide component preactivation has been developed. It includes the use of acetonitrile--quinoline (4:1) mixture as the solvent in coupling reactions. The efficiency of the method is illustrated by the synthesis of oligodeoxynucleotides.
Efimov
V A
VA
Dubeĭ
I Ia
IIa
D001483:Base Sequence; D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D008722:Methods; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000138:chemical synthesis*; D063065:Organophosphonates; D010766:Phosphorylation; D011804:Quinolines
D009841:Oligonucleotides; D063065:Organophosphonates; D011804:Quinolines; C037219:quinoline
Modifikatsiia H-fosfonatnogo metoda sinteza oligonukleotidov na polimernykh nositeliakh.