The pyrimidine/purine-biased region of the epidermal growth factor receptor gene is sensitive to S1 nuclease and may form an intramolecular triplex.
The pyrimidine/purine-biased region located upstream of the EGF (epidermal growth factor) receptor gene transcription initiation sites was sensitive to S1 nuclease when under superhelical tension. The structural basis of this specific reactivity to S1 nuclease was probed by the use of diethyl pyrocarbonate. The patterns of modification suggested that the H-form proposed by Mirkin, Lyamichev, Drushlyak, Dobrynin, Filippov & Frank-Kamenetskii [Nature (London) (1987) 330, 495-497], which includes an intramolecular triplex and a single-stranded region, was the most plausible model for the sequence tested. The results of dimethyl sulphate modification also supported this model.
Kato
M
M
Department of Molecular Biology, Keio University School of Medicine, Tokyo, Japan.
Kudoh
J
J
Shimizu
N
N
D001483:Base Sequence; D004278:DNA, Superhelical / Q000378:metabolism*; D066246:ErbB Receptors / Q000235:genetics*; D008745:Methylation; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011685:Purine Nucleotides; D011742:Pyrimidine Nucleotides; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism*; D013379:Substrate Specificity; D013463:Sulfuric Acid Esters / Q000494:pharmacology
D004278:DNA, Superhelical; D011685:Purine Nucleotides; D011742:Pyrimidine Nucleotides; D013463:Sulfuric Acid Esters; D066246:ErbB Receptors; D015719:Single-Strand Specific DNA and RNA Endonucleases; C007482:dimethyl sulfate