title: A rapid and sensitive gas chromatographic analysis of methadone and its primary metabolite.
abstract: A method for identification and quantification of methadone and its primary metabolite (1,5-dimethyl-3,3-diphenyl-2-2-ethylidene pyrrolidine) in plasma by GC/NPD is presented. The procedure employs SKF 525 A as the internal standard and requires no derivatization. After a single-step extraction, analysis is achieved in 10 min. The limit of detection was found to be 2 an 3 ng/ml for methadone and its metabolite, respectively. This method appears to be rapid, sensitive and applicable to forensic and clinical toxicological analyses.
authors.affiliation: Institut de Médecine Légale, Strasbourg.
authors.forename: P
authors.initials: P
authors.lastname: Kintz
authors.forename: P
authors.initials: P
authors.lastname: Mangin
authors.forename: A A
authors.initials: AA
authors.lastname: Lugnier
authors.forename: A J
authors.initials: AJ
authors.lastname: Chaumont
mesh_terms: D002849:Chromatography, Gas; D006801:Humans; D008691:Methadone / Q000097:blood*; D008832:Microchemistry; D011725:Pyridines; D011759:Pyrrolidines / Q000097:blood*; D011786:Quality Control
chemical_list: D011725:Pyridines; D011759:Pyrrolidines; C013109:metapyrone; D008691:Methadone; C010724:2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine