Determination of a platelet activating factor antagonist (CV-3988): methodology and clinical application.
A high-performance liquid chromatographic method was developed for determination of the platelet activating factor antagonist CV-3988 in human plasma and urine. After development of a column extraction procedure without an internal standard, a more satisfactory organic extraction procedure was set up with amiodarone as internal standard. Linearity of the calibration curves was found in the range 0.0625-10 micrograms/ml CV-3988. Reproducibility was higher than 10% for the column extraction and lower than 10% for the organic extraction procedure. Recovery of CV-3988 from plasma averaged 81.7% for the column procedure and 40% for the organic extraction. Urine samples could be extracted only by the organic extraction procedure. The organic extraction procedure was applied to the determination of CV-3988 in plasma and urine samples after intravenous administration to normal volunteers.
Verbesselt
R
R
Department of Pharmacology, Katholieke Universiteit Leuven, Belgium.
Tjandra-Maga
T B
TB
De Schepper
P J
PJ
Itakura
K
K
Sugiro
T
T
D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007275:Injections, Intravenous; D010742:Phospholipid Ethers / Q000032:analysis* / Q000097:blood / Q000592:standards / Q000652:urine; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D010742:Phospholipid Ethers; D010972:Platelet Activating Factor; C037913:CV 3988