[The effect of structural-chemical characteristics of water-soluble polyelectrolyte complexes of ovalbumin on their immunological properties].
Immunogenicity of soluble protein antigens in the complexes with synthetic polyions may be regarded as depending both on the nature of polymer carrier and the structure of the protein-polyelectrolyte complex. The immunogenicity of stable soluble complexes of ovalbumin (OA) with polycation - quaternized poly-4-vinylpyridine (C-1) and copolymer of acrylic acid and 2-methyl-5-vinylpyridine (C-2) have been evaluated. Immunization of mice by C-1 have induced a vigorous formation of the anti-OA IgG antibodies and IgE homocytotropic antibodies, while immunogenicity of OA in C-2 was comparable with that of OA alone. The analysis of the structural-chemical features of the complexes investigated has shown that enhanced immunogenicity of C-1 may be due to (1) the non-homogeneous distribution of protein globulae among polycation macromolecules and to (2) the formation of complex with an asymmetrical structure, to (3) the high ability of C-1 to adsorb on a surface of the lymphoid cells and to induce a formation of intercellular aggregates. An enhancing of a stability and a size of C-2 in the presence of Cu2+ shows no influence on a immunogenicity of OA. An immunogenicity of both types of complexes does not depend upon the access of determinants of OA to antibodies so far as it has been shown that complex formation in both cases are not accompanied by an alteration of antigenicity and allergenicity of OA.
Mustafaev
M I
MI
Babakhin
A A
AA
Popov
A N
AN
Litvinov
I S
IS
Merkushov
A V
AV
Gushchin
I S
IS
D000180:Acrylic Resins; D000818:Animals; D000917:Antibody Formation; D004573:Electrolytes; D007114:Immunization; D046911:Macromolecular Substances; D051379:Mice; D010047:Ovalbumin / Q000276:immunology*; D011108:Polymers; D011145:Polyvinyls; D011205:Povidone / Q000031:analogs & derivatives; D012995:Solubility; D014612:Vaccines / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*
D000180:Acrylic Resins; D004573:Electrolytes; D046911:Macromolecular Substances; D011108:Polymers; D011145:Polyvinyls; D014612:Vaccines; D014614:Vaccines, Synthetic; C019535:poly(4-vinylpyridine); C043348:acrylic acid-N-vinylpyrrolidone copolymer; D010047:Ovalbumin; D011205:Povidone
O vliianii strukturno-khimicheskikh osobennosteĭ vodorastvorimykh poliélektrolitnykh kompleksov oval'bumina na ikh immunologicheskie svoĭstva.