The polymerase chain reaction for Mycoplasma pulmonis.
In vitro DNA amplification by polymerase chain reaction was examined to detect Mycoplasma pulmonis. A pair of synthetic oligonucleotide primers was constructed, and used to amplify a unique sequence of M. pulmonis DNA. Amplified products were detected by agarose gel electrophoresis and verified by blot hybridization with a synthetic oligonucleotide probe. This system detected cellular DNA of M. pulmonis but not M. arthritidis or M. neurolyticum, and thus appears to be useful for M. pulmonis diagnosis.
Harasawa
R
R
Division of Animal Research, Faculty of Medicine, University of Tokyo.
Koshimizu
K
K
Uemori
T
T
Takeda
O
O
Asada
K
K
Kato
I
I
D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis*; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction
D004269:DNA, Bacterial