title: [Studies on elimination of mycoplasmas from cell line cultures].
abstract: Mycoplasmas were effectively eliminated from contaminated LTK and CHO cell lines by means of the antibiotic Tiamutin (10 micrograms/ml), while Kanamycin and Gentamycin were less potential. An elimination procedure which involved the consecutive treatment of the cell cloning over a periods of 21-28 days was adopted. This procedure was effective when applied to cell lines contaminated with unidentified and partially non-cultivable strains of mycoplasmas which could be detected by electronic microscope.
authors.affiliation: Shanghai Institute of Biological Products, Ministry of Public Health.
authors.forename: H J
authors.initials: HJ
authors.lastname: Zhang
authors.forename: Q M
authors.initials: QM
authors.lastname: Shi
authors.forename: Q Q
authors.initials: QQ
authors.lastname: Hu
authors.forename: B G
authors.initials: BG
authors.lastname: He
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: X Y
authors.initials: XY
authors.lastname: Lu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002460:Cell Line; D002478:Cells, Cultured; D006224:Cricetinae; D004224:Diterpenes / Q000494:pharmacology; D005260:Female; D009174:Mycoplasma / Q000187:drug effects* / Q000302:isolation & purification; D010053:Ovary / Q000166:cytology
chemical_list: D000900:Anti-Bacterial Agents; D004224:Diterpenes; C014224:tiamulin