title: Hormonal control of proliferation in the Ishikawa endometrial adenocarcinoma cell line.
abstract: Steroid hormone regulation of proliferation of the Ishikawa human endometrial adenocarcinoma cell line was investigated in defined tissue culture medium. Oestradiol increased cell number following treatment for greater than 8 days; 4-OH tamoxifen, used alone, induced growth in a similar manner to oestradiol and was not antagonistic when used in combination with oestradiol. Progesterone decreased cell number 4 days after treatment but thereafter the effect was lost; the effect of progesterone was abolished in the presence of Phenol Red, consistent with the oestrogenic properties of this indicator. Oestradiol together with progesterone for greater than 8 days resulted in maximal growth and was preceded by an apparent increase in synthesis of a protein of molecular weight 36 kDa pI 8.
authors.affiliation: Royal Postgraduate Medical School, Institute of Obstetrics and Gynaecology, Hammersmith Hospital, London, England.
authors.forename: J D
authors.initials: JD
authors.lastname: Croxtall
authors.forename: M G
authors.initials: MG
authors.lastname: Elder
authors.forename: J O
authors.initials: JO
authors.lastname: White
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D004347:Drug Interactions; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D010637:Phenolsulfonphthalein / Q000494:pharmacology; D011374:Progesterone / Q000494:pharmacology*; D013629:Tamoxifen / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D014594:Uterine Neoplasms / Q000378:metabolism / Q000473:pathology*
chemical_list: D013629:Tamoxifen; D011374:Progesterone; D004958:Estradiol; D010637:Phenolsulfonphthalein