title: [Creation of an experimental cell culture vaccine against distemper using cell line 4647].
abstract: Experiment in susceptible animals demonstrated the effectiveness and safety of several samples of a distemper vaccine prepared in cell line 4647. Preparations of the vaccine virus variants generated in line 4647 after 10 passages at 34 degrees C, 37 degrees C and 40 degrees C and 20 passages of rapidly multiplying virus (34 degrees C) possessed the protective activity when used for immunization in a dose of 1000 PFU/animal. The results obtained in observations of the animals and examinations of sera for antibodies did not differ from those of control tests of "Vakchum".
authors.forename: V P
authors.initials: VP
authors.lastname: Grachev
authors.forename: A V
authors.initials: AV
authors.lastname: Kurbatov
authors.forename: L L
authors.initials: LL
authors.lastname: Mironova
authors.forename: V D
authors.initials: VD
authors.lastname: Popova
mesh_terms: D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004216:Distemper / Q000517:prevention & control*; D004217:Distemper Virus, Canine / Q000276:immunology* / Q000472:pathogenicity; D012889:Mephitidae; D014611:Vaccination / Q000662:veterinary*; D014765:Viral Vaccines / Q000096:biosynthesis*; D014774:Virulence
chemical_list: D014765:Viral Vaccines
vernacular_title: Sozdanie éksperimental'noĭ kul'tural'noĭ vaktsiny protiv chumy plotoiadnykh s ispol'zovaniem kletok 4647.