Isolated Coxiella burnetii synthesizes DNA during acid activation in the absence of host cells.
Two populations of Coxiella burnetii were isolated from fibroblast tissue cultures and examined for their ability to synthesize DNA when incubated in a defined medium. Both the populations released by mechanical lysis of heavily infected host cells, as well as those recovered from the tissue culture medium, incorporated H3 32PO4 into DNA. Incorporation occurred at pH 4.5 but not at pH 7.0, and proceeded for 12-15 h. When incorporation of [3H]thymidine was studied, only the organisms obtained by mechanical lysis of host cells were active. Those which had been released by natural means into the tissue culture medium, and then recovered for study, did not incorporate precursor thymidine but were extremely active in protein biosynthesis. In mechanically released organisms, thymidine incorporation was inhibited immediately by rifamycin (40 microM) and hydroxyurea (10 mM), but it was not affected by chloramphenicol (310 microM) until 4 h after addition of the drug. Incorporation of H3 32PO4 by both populations of organisms was also inhibited by rifamycin, chloramphenicol and hydroxyurea, but the time sequence of inhibition differed. Southern hybridization utilizing 32P-labelled DNA suggested that both populations synthesized authentic chromosomal DNA sequences, as well as QpH1 plasmid DNA, during acid activation of metabolism.
Chen
S Y
SY
Department of Microbiology and Immunology, School of Medicine, West Virginia University, Morgantown 26506.
Vodkin
M
M
Thompson
H A
HA
Williams
J C
JC
D015139:Blotting, Southern; D002701:Chloramphenicol / Q000494:pharmacology; D003381:Coxiella / Q000187:drug effects / Q000378:metabolism*; D003470:Culture Media; D004269:DNA, Bacterial / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D006918:Hydroxyurea / Q000494:pharmacology; D012294:Rifamycins / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism
D003470:Culture Media; D004269:DNA, Bacterial; D012294:Rifamycins; D002701:Chloramphenicol; D013936:Thymidine; D006918:Hydroxyurea