title: High-performance liquid chromatography of sulphadimethoxine and its N1-glucuronide, N4-acetyl and N4-acetyl-N1-glucuronide metabolites in human plasma and urine.
abstract: Sulphadimethoxine is metabolized in humans by N1-glucuronidation and by N4-acetylation. Sulphadimethoxine-N1-glucuronide can be measured by the direct high-performance liquid chromatographic analysis and without enzymic deglucuronidation. The N1-glucuronide can be measured by an isocratic as well as by a gradient mobile phase. The group contribution of the N1-glucuronide moiety to the capacity factor is a reduction of 0.24 in the isocratic system and 0.55 in the gradient system. N4-Acetylation increases the capacity factor by a factor 1.4 in the isocratic system and by 1.06 in the gradient system.
authors.affiliation: Department of Clinical Pharmacy, Sint Radboud University Hospital, Nijmegen, The Netherlands.
authors.forename: T B
authors.initials: TB
authors.lastname: Vree
authors.forename: E W
authors.initials: EW
authors.lastname: Kolmer
authors.forename: M
authors.initials: M
authors.lastname: Martea
authors.forename: R
authors.initials: R
authors.lastname: Bosch
authors.forename: M
authors.initials: M
authors.lastname: Shimoda
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005965:Glucuronates / Q000097:blood* / Q000378:metabolism / Q000652:urine; D006801:Humans; D013412:Sulfadimethoxine / Q000097:blood* / Q000378:metabolism / Q000652:urine
chemical_list: D005965:Glucuronates; D013412:Sulfadimethoxine