pH standardization for phosphorus-31 magnetic resonance heart spectroscopy at different temperatures.
Intracellular pH typically is measured by NMR using the calibrated chemical shift of the inorganic phosphate peak in phosphorus-31 spectra. Heart spectroscopy experiments often require measurements of intracellular pH at temperatures from 5 to 37 degrees C. This paper provides NMR pH calibrations for this range of temperatures, a summary of calibration data reported to date, and a discussion of the factors influencing pH standardization.
Kost
G J
GJ
Clinical Chemistry, School of Medicine, University of California, Davis 95616.
D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D009206:Myocardium / Q000032:analysis*; D010758:Phosphorus / Q000032:analysis*; D013696:Temperature
D010758:Phosphorus