Extraction with methyl tert-butyl ether overcomes erratic elution patterns of 6-keto-prostaglandin F1 alpha on high pressure liquid chromatography.
Solvent extraction of 6-keto-PGF1 alpha from aqueous solutions with ethyl acetate was found to result in variable and irreproducible elution patterns, when the extracts were subjected to high pressure liquid chromatography. These problems could not be resolved satisfactorily by using ethyl acetate from different suppliers, nor by changing acids or pH for acidification. After a number of unsuccessful attempts to resolve this problem, we found that variable and irreproducible elution patterns could be avoided by using methyl t-butyl ether as extraction solvent.
Noort
W A
WA
Department of Obstetrics and Gynecology, Leiden University Hospital, The Netherlands.
de Zwart
F A
FA
Keirse
M J
MJ
D015121:6-Ketoprostaglandin F1 alpha / Q000032:analysis* / Q000652:urine; D000085:Acetates; D000653:Amniotic Fluid / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004987:Ethers; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008738:Methyl Ethers; D011247:Pregnancy; D012997:Solvents; D014867:Water
D000085:Acetates; D004987:Ethers; D008738:Methyl Ethers; D012997:Solvents; D014867:Water; C043243:methyl tert-butyl ether; D015121:6-Ketoprostaglandin F1 alpha; C007650:ethyl acetate