Lipopolysaccharide (LPS) alters phosphatidylcholine metabolism in elicited peritoneal macrophages.
We investigated the effects of LPS on mouse peritoneal macrophage phospholipids using radiolabeled precursors. LPS (200 ng/ml) stimulated incorporation of [32P] into all classes of phospholipids within 0.5 hr, and after 2 hr the increase was 60% greater than controls. Separation of the phospholipid classes by thin-layer chromatography revealed a selective increase in incorporation of label into phosphatidylcholine (PC) (90% increase compared to approximately 50% in the other phospholipids). In macrophages labeled with [3H]-choline, LPS stimulated both the incorporation of label into PC and the release of incorporated label into the medium. The time dependencies of stimulated [3H] release and [32P] incorporation were similar. These data are consistent with the hypothesis that LPS activates macrophages via a PC-specific phospholipase-dependent mechanism.
Grove
R I
RI
Bristol-Myers Company, PRDD, Wallingford, Connecticut 06492.
Allegretto
N J
NJ
Kiener
P A
PA
Warr
G A
GA
D000818:Animals; D002478:Cells, Cultured; D002794:Choline / Q000378:metabolism; D005260:Female; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology*; D010713:Phosphatidylcholines / Q000378:metabolism*; D010740:Phospholipases / Q000502:physiology; D010761:Phosphorus Radioisotopes; D013997:Time Factors; D014316:Tritium
D008070:Lipopolysaccharides; D010713:Phosphatidylcholines; D010761:Phosphorus Radioisotopes; D014316:Tritium; D010740:Phospholipases; D002794:Choline