[A simple and sensitive method of determination of superoxide dismutase activity based on the reaction of quercetin oxidation].
An indirect method for determination of superoxide dismutase (SOD) activity, based on inhibition of quercetin autooxidation, was developed. The optimal conditions of reaction ensuring high sensitivity of measurement (I50 = 1.5 ng/ml) were selected. NaN3, catalase and physiological concentrations of ascorbic acid did not interfere with the SOD estimation in blood and tissue homogenates.
Kostiuk
V A
VA
Potapovich
A I
AI
Kovaleva
Zh V
ZhV
D000818:Animals; D005260:Female; D005419:Flavonoids; D006801:Humans; D008297:Male; D010084:Oxidation-Reduction; D011794:Quercetin / Q000378:metabolism; D051381:Rats; D013482:Superoxide Dismutase / Q000032:analysis* / Q000097:blood; D014018:Tissue Distribution
D005419:Flavonoids; D011794:Quercetin; D013482:Superoxide Dismutase
Prostoĭ i chuvstvitel'nyĭ metod opredeleniia aktivnosti superoksid- dismutazy, osnovannyĭ na reaktsii okisleniia kvertsetina.