Radiation effect in mouse skin: dose fractionation and wound healing.
Radiation induced dermal injury was measured by the gain in the physical strength of healing wounds in mouse skin. A sigmoid dose response for the inhibition of wound healing 14 days after surgery was found for single doses of X rays. The sparing of dermal damage from fractionation of the X-ray dose was quantified in terms of the alpha/beta ratio in the linear-quadratic (LQ) model, at a wide range of doses per fraction reaching as low as about 1 Gy. The fit and the appropriateness of the LQ model for the skin wound healing assay was examined with the use of the Fe-plot in which inverse total dose is plotted versus dose per fraction for wound strength isoeffects. The alpha/beta ratio of the skin was about 2.5 Gy (95% confidence of less than +/- 1 Gy) and was appropriate over a dose range of 1 Gy to about 8 Gy. The low alpha/beta value is typical for a late responding tissue. This assay, therefore, has the advantage of measuring and forecasting late radiation responses of the dermis within a short time after irradiation.
Gorodetsky
R
R
Sharrett Institute of Oncology, Haddassah University Hospital, Jerusalem, Israel.
Mou
X D
XD
Fisher
D R
DR
Taylor
J M
JM
Withers
H R
HR
D000818:Animals; D004307:Dose-Response Relationship, Radiation; D005260:Female; D051379:Mice; D012867:Skin / Q000528:radiation effects*; D014945:Wound Healing / Q000528:radiation effects*