title: Genomic organization of the mouse T cell receptor V alpha family.
abstract: Based on the analysis of V alpha gene segment deletions in a panel of T lymphomas, we have constructed a map of the mouse T cell receptor alpha/delta region and assigned the relative position of 72 distinct V gene segments. Three major observations have emerged from such studies. First, members of a given V alpha subfamily are not organized in discrete units along the chromosome but largely interspersed with members of other V alpha subfamilies. Second, analysis of the deletion map suggests the existence of repetitive patterns (V alpha clusters) in the chromosomal distribution of the V alpha gene segments. Third, the present-day organization of the V alpha/delta region may be readily explained by a series of sequential duplications involving three ancestral V alpha clusters. Direct evidence for the existence of these unique structural features has been gained by cloning approximately 370 kb of DNA and positioning 26 distinct V alpha gene segments belonging to six different subfamilies. Finally, the relationships existing between the V alpha/delta gene segment organization and usage are discussed in terms of position-dependent models.
authors.affiliation: Département d'Immunologie, Institut Pasteur (CNRS LA 359), Paris, France.
authors.forename: E
authors.initials: E
authors.lastname: Jouvin-Marche
authors.forename: I
authors.initials: I
authors.lastname: Hue
authors.forename: P N
authors.initials: PN
authors.lastname: Marche
authors.forename: C
authors.initials: C
authors.lastname: Liebe-Gris
authors.forename: J P
authors.initials: JP
authors.lastname: Marolleau
authors.forename: B
authors.initials: B
authors.lastname: Malissen
authors.forename: P A
authors.initials: PA
authors.lastname: Cazenave
authors.forename: M
authors.initials: M
authors.lastname: Malissen
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002460:Cell Line; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D015329:Gene Rearrangement, T-Lymphocyte; D008223:Lymphoma / Q000276:immunology; D051379:Mice; D005810:Multigene Family; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D004273:DNA, Neoplasm; D011948:Receptors, Antigen, T-Cell