Forssman antigen expressed on lymph node cells of MRL/MpJ-lpr/lpr mice is of a glycoprotein nature.
This paper reports the nature of abnormally expressed Forssman (F) antigen in the lymph node cells of MRL/MpJ-lpr/lpr, autoimmune mice, and also reports its autoantibody in sera. By acetylation study of the F antigen with [14C]acetic anhydride, we concluded that the F antigen was not a glycolipid but a glycoprotein. Several bands of F-active glycoproteins were identified on a nitrocellulose sheet after purification by an anti-F antibody affinity column. Hemolysis of SRBC by some sera from MRL/MpJ/lpr/lpr was inhibited by purified F glycoprotein and also by F glycolipid. The antibody in the serum, however, seemed to be more specific for F glycoproteins than F glycolipid, but the opposite was the case for rabbit anti-F glycolipid antibody. No significant difference of the SRBC hemolysis levels was observed between the sera from MRL/MpJ-lpr/lpr and its congenic MRL/MpJ-+/+ mice.
Kijimoto-Ochiai
S
S
Institute of Immunological Science, Hokkaido University.
Tashiro
A
A
Katagiri
Y U
YU
Hatae
T
T
Kobayashi
S
S
Okuyama
H
H
D000089:Acetic Anhydrides / Q000378:metabolism; D000107:Acetylation; D000818:Animals; D015478:Antigens, Heterophile / Q000032:analysis*; D001323:Autoantibodies / Q000276:immunology; D002247:Carbon Isotopes; D002478:Cells, Cultured; D005577:Forssman Antigen / Q000032:analysis*; D005915:Globosides / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis*; D006028:Glycosphingolipids / Q000032:analysis*; D006461:Hemolysis; D006801:Humans; D008198:Lymph Nodes / Q000032:analysis / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H
D000089:Acetic Anhydrides; D015478:Antigens, Heterophile; D001323:Autoantibodies; D002247:Carbon Isotopes; D005915:Globosides; D006023:Glycoproteins; D006028:Glycosphingolipids; C010163:Forssman glycolipid; D005577:Forssman Antigen