[Lactate dehydrogenase and malate dehydrogenase activity in the glial and neuronal fractions of the brain tissue in rats of various ages].
The authors investigated in Wistar strain rats of both sexes aged 10 days and in adult rats (aged 90 days) the activity of lactic dehydrogenase (E.C.1.1.1.27) and malic dehydrogenase (E.C.1.1.1.37) in the neuronal and glial fraction of the cerebral cortex. They found that during maturation the LDH activity declines in the neuronal fraction in a significant way (expressed per mg protein). The LDH activity in the neuronal fraction is always significantly higher than in the glial fraction. The G/N index declines slightly during development (from 0.30 to 0.23). In malic dehydrogenase the authors found also a decline of this activity in neuronal tissue during maturation, while in the glial fraction the activity of this enzyme does not change significantly. The G/N index increased as a result of the described developmental changes from 0.33 in 10-day-old rats to 0.55 in adult ones. The authors used for the isolation of the neuronal and glial fraction the method described by Lisý. LDH and MDH were estimated by previously described methods.
Hrachovina
V
V
Fyziologický ústav fakulty vseobecného lékarství Univerzity Karlovy, Praha.
Mourek
J
J
D000375:Aging / Q000378:metabolism*; D000818:Animals; D002540:Cerebral Cortex / Q000166:cytology / Q000201:enzymology*; D005260:Female; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008291:Malate Dehydrogenase / Q000378:metabolism*; D008297:Male; D009457:Neuroglia / Q000201:enzymology; D009474:Neurons / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains
D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase
Aktivita laktát a malátdehydrogenázy v gliální a neuronální frakci mozkové tkánĕ různĕ starých krys.