HELP (high efficiency liquid phase) new oligonucleotide synthesis on soluble polymeric support.
A simple, rapid and high-yielding method for the synthesis of oligonucleotides by the phosphotriesters approach is described. The use of polyethylene glycol (PEG) as soluble polymeric support preserves some convenient features of the solid-phase synthesis with new interesting advantages. Short oligonucleotides in hundred milligrams scale can be obtained from few grams of functionalized PEG.
Bonora
G M
GM
Department of Organic Chemistry, University of Padova, Italy.
Scremin
C L
CL
Colonna
F P
FP
Garbesi
A
A
D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D009682:Magnetic Resonance Spectroscopy; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000302:isolation & purification; D011092:Polyethylene Glycols; D012995:Solubility
D009841:Oligonucleotides; D011092:Polyethylene Glycols