title: Strongyloides stercoralis: antigenic analysis of infective larvae and adult worms.
abstract: The protein composition of Strongyloides stercoralis infective larvae and adult worms solubilized sequentially in water, sodium deoxycholate and sodium dodecyl sulphate (SDS) and their excretory/secretory products were analysed by one- and two-dimensional SDS-polyacrylamide electrophoresis. These extracts were demonstrated to be complex mixtures containing many proteins, some of which were common and others which were stage-specific. Western blot analysis of these antigens with infected human sera showed most sero-reactivity against larval antigens, whilst normal human sera were unreactive. These data identify immunogenic antigens which may be available for detection in an antigen assay.
authors.affiliation: Department of Medicine, University of Western Australia, Nedlands.
authors.forename: C
authors.initials: C
authors.lastname: Northern
authors.forename: D I
authors.initials: DI
authors.lastname: Grove
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007814:Larva / Q000276:immunology; D008970:Molecular Weight; D013321:Strongyloides / Q000276:immunology*
chemical_list: D000947:Antigens, Helminth