Application of adsorptive voltammetry to assess the stability of modified alanine aminotransferases.
Alanine aminotransferase has been stabilized by using chemical modification with both bis(imidates) (of varying length) and succinic anhydride. The voltammetric behavior of the native enzyme and its various modified forms has been studied by using both cyclic voltammetry and differential pulse adsorptive voltammetry. A distinctive accumulation pattern was found for each of the stabilized enzymes at the static mercury drop electrode with respect to the native alanine aminotransferase. Adsorptive voltammetry was demonstrated to be a useful technique to assess the extent of chemical modification of this enzyme, which is indirectly related to their stability for use in biotechnological processes. The sue of differential pulse adsorptive voltammetry, after a preconcentration of the enzyme for 300 s at the electrode surface, has yielded a detection limit of 1.0 x 10(-9) M.
Fernández Alvarez
J M
JM
School of Chemical Sciences, Dublin City University, Ireland.
O'Fagain
C
C
O'Kennedy
R
R
Kilty
C G
CG
Smyth
M R
MR
D000410:Alanine Transaminase / Q000032:analysis*; D004563:Electrochemistry; D004795:Enzyme Stability; D006801:Humans; D013056:Spectrophotometry, Ultraviolet
D000410:Alanine Transaminase