Detection by polymerase chain reaction amplification of human herpesvirus 6 DNA in peripheral blood of patients with exanthem subitum.
The polymerase chain reaction was used to detect human herpesvirus 6 (HHV-6) DNA in peripheral blood mononuclear cells from patients with exanthem subitum. Amplified products were detected by agarose gel electrophoresis and dot blot hybridization with a cloned DNA probe. No cross-hybridization with DNAs of five other human herpesviruses was observed in this system, and all HHV-6 strains gave positive reactions when the primer pairs were used. Immunoglobulin M antibody appeared about 5 days after the onset of disease, reaching a maximum after about 2 to 3 weeks and then decreasing to less than 1:10 1 month after the onset of disease in almost all patients. Mononuclear cells from seven patients with exanthem subitum all gave positive reactions in this system, and viral DNA was found in samples even during the convalescent phase of the disease. We conclude that this test, using polymerase chain reaction amplification, which takes only 1 to 2 days, is useful for the diagnosis of HHV-6 infection.
Kondo
K
K
Department of Virology, Osaka University, Japan.
Hayakawa
Y
Y
Mori
H
H
Sato
S
S
Kondo
T
T
Takahashi
K
K
Minamishima
Y
Y
Takahashi
M
M
Yamanishi
K
K
D000914:Antibodies, Viral / Q000097:blood; D004279:DNA, Viral / Q000097:blood*; D005077:Exanthema Subitum / Q000097:blood / Q000276:immunology / Q000382:microbiology*; D005784:Gene Amplification; D006564:Herpesviridae / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000382:microbiology; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D013997:Time Factors
D000914:Antibodies, Viral; D004279:DNA, Viral