The 8-hydroxyguanine content of isolated mitochondria increases with lipid peroxidation.
When lipid peroxidation was induced in isolated mitochondria there was a marked increase in the 8-hydroxyguanine content of the nucleic acids extracted from these mitochondria. The elevation of 8-hydroxyguanine levels was associated with an extensive alteration of normal electrophoretic mobility of mitochondrial DNA. However, suppression of lipid peroxidation with alpha-tocopherol proportionally attenuated 8-hydroxyguanine production and limited the electrophoretic mobility change of mitochondrial DNA.
Hruszkewycz
A M
AM
Department of Pathology, George Washington University, Medical Center, Washington, DC 20037.
Bergtold
D S
DS
D000818:Animals; D004249:DNA Damage; D004272:DNA, Mitochondrial / Q000032:analysis / Q000187:drug effects* / Q000378:metabolism; D006147:Guanine / Q000031:analogs & derivatives* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D004272:DNA, Mitochondrial; D008563:Membrane Lipids; C024829:8-hydroxyguanine; D006147:Guanine