Calcium-ion binding by the potential calcium-ion-binding protein, p9Ka.
p9Ka is a polypeptide of apparent molecular mass 9 kDa, present in cultured rat mammary myoepithelial-like cells, but virtually absent in their parental epithelial cells. mRNA for p9Ka is present in normal rodent tissues. The amino acid sequence of a protein of molecular mass 12 KDa, derived from the nucleotide sequence of the p9Ka gene, is related to that of S-100 protein, a calcium-ion-binding protein. p9Ka, isolated from cultured rat mammary myoepithelial-like cells is now shown to bind calcium ions in vitro suggesting that the derived amino acid sequence is correct, and that an apparent discrepancy between the molecular masses of the predicted and isolated p9Ka does not affect this activity.
Barraclough
R
R
Department of Biochemistry, University of Liverpool, United Kingdom.
Gibbs
F
F
Smith
J A
JA
Haynes
G A
GA
Rudland
P S
PS
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D004848:Epithelium; D005260:Female; D008321:Mammary Glands, Animal; D008970:Molecular Weight; D011485:Protein Binding; D051381:Rats
D002135:Calcium-Binding Proteins; D002118:Calcium