Transcriptional regulation of an aldolase gene in the regenerating rat liver.
The relative abundance of rat aldolase A, B, C, alpha-fetoprotein, and albumin mRNAs was determined by Northern hybridization during liver regeneration after partial hepatectomy. Aldolase A mRNA increased more than 10-fold on the 3rd day after partial resection compared with that of normal adult rat liver. S1 analysis revealed that three species of aldolase A mRNAs (mRNA I, II & III) reappeared. However, transcriptional rate of aldolase A mRNA did not change at all during the regeneration. In contrast, aldolase B, aldolase C and albumin mRNAs did not change at all. These findings suggest that the differentiated hepatocyte maintains a differentiated state during the liver regeneration as seen in aldolase B, whereas "oncofetal" isozymes such as aldolase A resurge after partial hepatectomy under the control of post-transcriptional mechanism.
Motomura
M
M
Department of Biochemistry, Saga Medical School, Japan.
Mukai
T
T
Ozaki
I
I
Joh
K
K
Arai
Y
Y
Sakai
T
T
Hori
K
K
D000418:Albumins / Q000235:genetics; D000818:Animals; D015152:Blotting, Northern; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics*; D008115:Liver Regeneration / Q000235:genetics*; D008297:Male; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014158:Transcription, Genetic
D000418:Albumins; D012333:RNA, Messenger; D005634:Fructose-Bisphosphate Aldolase