Radioactive gold cluster immunoconjugates: potential agents for cancer therapy.
An 11 gold atom (undecagold) cluster was covalently attached to specific sites on Fab', F(ab')2 and whole IgG molecules such that each carried 11-33 gold atoms without significant loss of native immunospecificity. Gold cluster labeled 17-1A monoclonal F(ab')2 antibody fragments showed 80% immunoreactivity compared to native antibody fragments in binding to human colon carcinoma cells in vitro. Radioactive gold in vivo biodistributions in nude mice with human tumors are also reported. By using clusters, potentially a larger destructive payload can be carried per antibody.
Hainfeld
J F
JF
Biology Department, Brookhaven National Laboratory, Upton, NY 11973.
Foley
C J
CJ
Srivastava
S C
SC
Mausner
L F
LF
Feng
N I
NI
Meinken
G E
GE
Steplewski
Z
Z
D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics / Q000627:therapeutic use*; D003110:Colonic Neoplasms / Q000378:metabolism; D006050:Gold Radioisotopes / Q000008:administration & dosage / Q000627:therapeutic use*; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000493:pharmacokinetics / Q000627:therapeutic use; D066298:In Vitro Techniques; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000532:radiotherapy*; D014018:Tissue Distribution; D014183:Transplantation, Heterologous
D000911:Antibodies, Monoclonal; D006050:Gold Radioisotopes; D007140:Immunoglobulin Fab Fragments