cDNA cloning of somatolactin, a pituitary protein related to growth hormone and prolactin.
From a flounder pituitary cDNA library, cDNA clones encoding a 28-kDa glycoprotein produced by the pars intermedia of the pituitary were isolated and characterized. Nucleotide sequencing demonstrated a precursor of the 28-kDa protein, which consisted of 231 amino acid residues, to be cleaved into a signal peptide (24 amino acids) and a mature protein (207 amino acids) containing one N-glycosylation site. By comparison of amino acid sequences, the 28-kDa protein was found to be distantly and similarly related to growth hormone and prolactin. Consequently, it was named somatolactin. Somatolactin mRNAs were specifically expressed as 1.2 and 1.8 kb poly(A)+ RNAs in flounder pituitary.
Ono
M
M
Department of Molecular Biology, School of Medicine, Kitasato University, Kanagawa, Japan.
Takayama
Y
Y
Rand-Weaver
M
M
Sakata
S
S
Yasunaga
T
T
Noso
T
T
Kawauchi
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D029941:Fish Proteins; D005432:Flounder; D015870:Gene Expression; D005796:Genes; D006023:Glycoproteins / Q000235:genetics*; D013006:Growth Hormone / Q000235:genetics; D008969:Molecular Sequence Data; D010907:Pituitary Hormones / Q000235:genetics*; D011388:Prolactin / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping
D029941:Fish Proteins; D006023:Glycoproteins; D010907:Pituitary Hormones; D012333:RNA, Messenger; C064448:somatolactin protein, fish; D011388:Prolactin; D013006:Growth Hormone; D004247:DNA