High hemoglobin mixed disulfide content in hemolysates from stressed shark.
1. Hemolysate from heavily stressed smooth hammerhead shark, Sphyrna zygaena, shows three electrophoretic components, SZ I, SZ II and SZ III, whose relative concentrations are 36.4 +/- 6.8, 36.4 +/- 5.0 and 20.8 +/- 5.7%, respectively. After reduction with DTE only SZ I remained. 2. SZ I reacted with glutathione disulfide reconstitute SZ II and SZ III. 3. Non-reduced, DTE-reduced, and denatured hemoglobin were found to have 2.0 +/- 0.4, 3.7 +/- 0.6, and 9.4 +/- 0.7-SH groups, respectively. 4. Erythrocyte non-protein--SH (NPSH), including glutathione present as mixed disulfide with SZ II and SZ III, is 1.7 NPSH/Hb.
Dafré
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Departamento Fisiologia, Farmacologia e Biofisica, UFRGS, Porto Alegre, Brasil.
Reischl
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D000293:Adolescent; D000818:Animals; D002850:Chromatography, Gel; D004220:Disulfides / Q000032:analysis; D004229:Dithiothreitol / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004592:Electrophoresis, Starch Gel; D006454:Hemoglobins / Q000302:isolation & purification*; D006461:Hemolysis; D006801:Humans; D046911:Macromolecular Substances; D012754:Sharks; D013312:Stress, Physiological / Q000097:blood*
D004220:Disulfides; D006454:Hemoglobins; D046911:Macromolecular Substances; D004229:Dithiothreitol