Complexes of nuclear matrix DNA with proteins tightly bound to DNA contain a specific small-size RNA of a novel type.
Analysis of DNA-protein structures composed of nuclear matrix attached DNA and the most tightly bound proteins was performed. Although the previously described non-histone proteins (1) were present the buoyant density of the complex was the same as that of pure DNA. RNA inaccessible to RNase in 0.4 M NaCl but digestible in low ionic strength buffer was detected. This RNA is not a nascent one. It turned out to be homogeneous and represent a novel type of small nuclear RNA. Partial sequence of this RNA is presented.
Chernokhvostov
V V
VV
Department of Nucleic Acids Biosynthesis, USSR Academy of Sciences, Moscow.
Georgiev
G P
GP
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis; D004268:DNA-Binding Proteins / Q000032:analysis*; D051379:Mice; D008969:Molecular Sequence Data; D015530:Nuclear Matrix / Q000032:analysis*; D010954:Plasmacytoma / Q000032:analysis; D012342:RNA, Small Nuclear / Q000032:analysis*; D014407:Tumor Cells, Cultured / Q000032:analysis
D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; D012342:RNA, Small Nuclear; D004247:DNA