[Synthesis of ovalbumin and globin during simultaneous translation of their mRNA in the presence of tRNA of different origin in the cell-free protein-synthesizing system from wheat germ].
Competition among ovalbumin and globin mRNA under their simultaneous translation in the tRNA-dependent cell-free system from wheat germ was studied. One of the mRNAs was added to samples in a constant amount, that provided 50% protein synthesis level of the maximum, other--in increasing amount (from 0 to the maximum). The ration of ovalbumin and globin synthesis rates has been shown to depend essentially (1.5-2 fold) on the nature of tRNA, being added to the system. The obtained data suggest that functional adaptation of tRNA is a part of the mechanism of protein biosynthesis regulation and it is possible to modulate some mRNAs translation selectivity on the elongation by different sets of tRNA.
Rachkov
A E
AE
Shul'ga
N I
NI
Starodub
N F
NF
El'skaia
A V
AV
D000818:Animals; D002474:Cell-Free System; D002645:Chickens; D005914:Globins / Q000096:biosynthesis* / Q000235:genetics; D010047:Ovalbumin / Q000096:biosynthesis* / Q000235:genetics; D010057:Oviducts / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D014908:Triticum / Q000378:metabolism
D012333:RNA, Messenger; D005914:Globins; D010047:Ovalbumin; D012343:RNA, Transfer
Sintez oval'bumina i globina pri odnovremennoĭ transliatsii ikh mRNK v prisutstvii tRNK razlichnogo proiskhozhdeniia v beskletochnoĭ beloksinteziruiushcheĭ sisteme iz zarodysheĭ pshenitsy