The use of Stokes Raman scattering in time correlated single photon counting: application to the fluorescence lifetime of tyrosinate.
The decay kinetics of the tyrosinate form of tyrosine in aqueous solution, pH 11, were investigated by time correlated single photon counting using laser excitation. The lifetime of the single exponential decay was 30.5 +/- 0.2 ps. Stokes Raman scattering was used to determine the instrument response function and the advantages of this approach over the conventional Rayleigh scattering method are discussed.
Willis
K J
KJ
Division of Biological Sciences, National Research Council of Canada, Ottawa, Ontario.
Szabo
A G
AG
Krajcarski
D T
DT
D007700:Kinetics; D011827:Radiation; D012972:Sodium Hydroxide; D012996:Solutions; D013050:Spectrometry, Fluorescence / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014443:Tyrosine
D012996:Solutions; D014443:Tyrosine; D012972:Sodium Hydroxide