Xenotropic and amphotropic pseudotyped recombinant retrovirus to transfer genes into cells from various species.
The ability to transfer genes into cells from different species with murine recombinant retroviruses was evaluated with the SVnls LacZ reporter gene. Mouse and cat packaging cell lines can be used to transfer amphotropic pseudotype, in human, mouse, cat, rabbit, sheep, horse and beef cells and with a very low efficiency in pig and avian cells. Xenotropic pseudotype recombinant retroviruses, produced in cat and rabbit packaging cell lines, transferred genes with the same efficiency as amphotropic retroviruses in human, cat, rabbit and sheep cells. In contrast to amphotropic retroviruses, xenotropic retroviruses infect beef, pig and horse cells with a high efficiency. These results emphasize the need to determine carefully the producer cell line (the type of helper virus and the species origin of the cell) for efficient transfer of genes in cells and embryos.
Delouis
C
C
INRA, Station de Physiologie animale, Jouy-en-Josas, France.
Milan
D
D
L'Haridon
R
R
Gianquinto
L
L
Bonnerot
C
C
Nicolas
J F
JF
D000818:Animals; D002460:Cell Line; D006378:Helper Viruses / Q000502:physiology; D006801:Humans; D011995:Recombination, Genetic; D012190:Retroviridae / Q000235:genetics* / Q000502:physiology; D013045:Species Specificity; D014162:Transfection / Q000235:genetics*; D014779:Virus Replication