Different ionic current patterns of acutely isolated neurons from the terminal ganglion of the cockroach Periplaneta americana L.
Ionic currents of somata, acutely isolated from the terminal ganglion of the cockroach Periplaneta americana, were investigated by the intracellular perfusion technique. By the ratios of the amplitudes of the fast inward current to the stationary outward current all isolated neurons are classified into three groups. State 1 neurons are characterized by a large amplitude of the fast Na current. At its maximum (-20 mV) the stationary outward current is small. In state 3 neurons, an inward current could not be observed at any potential, but the stationary component of the outward current at -20 mV is already large. State 2 neurons represent an intermediate type. In state 3 neurons, no fast Na current could be observed even after elimination of the outward currents electrochemically (substitution of K ions by Tris ions) or pharmacologically (application of 2 mmol/l 4-aminopyridine and 50 mmol/l TEA-Cl). Instead of this, a Ca current was recorded although fluoride was used as the intracellular anion.
Gündel
J
J
Institute for Neurobiology and Brain Research, Academy of Sciences of GDR, Magdeburg.
Felsmann
A
A
Matthies
H
H
D000818:Animals; D003058:Cockroaches / Q000502:physiology*; D005724:Ganglia / Q000378:metabolism*; D066298:In Vitro Techniques; D008564:Membrane Potentials; D009474:Neurons / Q000378:metabolism*; D015203:Reproducibility of Results; D012996:Solutions
D012996:Solutions