Electrophoresis in an expanded stationary boundary.
A method of electrophoresis in an expanded stationary boundary is described, in which a pH gradient formed in a single buffer is used. The degrees of ionization and the concentrations of the electrolytes have values at which the polyions stack and resolve simultaneously in a polyacrylamide gel. To illustrate the method, the resolving of serum proteins in a pH gradient formed by Tris-glycinate buffer is considered.
Michov
B M
BM
Department of Biochemistry, Academy of Medicine, Sofia.
D001798:Blood Proteins / Q000032:analysis; D002021:Buffers; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005998:Glycine; D006863:Hydrogen-Ion Concentration; D012984:Software; D014325:Tromethamine
D001798:Blood Proteins; D002021:Buffers; D014325:Tromethamine; D005998:Glycine