An alternative expeditious analysis of phospholipid composition in human blood plasma by 31P NMR spectroscopy.
31P nuclear magnetic resonance spectroscopy has been applied to quantitate phospholipids in human blood plasma and in separate lipoprotein fractions. The addition of suitable detergents to samples produces an excellent chemical shift dispersion and allows the identification and integration of the peaks of the most important phospholipids. Results are in agreement with those obtained with enzymatic colorimetric and TLC methods: our method is characterized by good accuracy and reproducibility.
Bradamante
S
S
C.N.R. e Dipartimemto di Chimica Organica e Industriale, Università di Milano, Italy.
Barchiesi
E
E
Barenghi
L
L
Zoppi
F
F
D000293:Adolescent; D000328:Adult; D000368:Aged; D003902:Detergents / Q000494:pharmacology; D004164:Diphosphonates; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008563:Membrane Lipids / Q000097:blood; D008875:Middle Aged; D010743:Phospholipids / Q000097:blood*; D010758:Phosphorus; D012016:Reference Values
D003902:Detergents; D004164:Diphosphonates; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D008563:Membrane Lipids; D010743:Phospholipids; D010758:Phosphorus; C027474:methylene diphosphonate