Vitamin K-dependent carboxylase: structural requirements for propeptide activation.
The activity of the rat liver vitamin K-dependent gamma-glutamyl carboxylase has been shown to be stimulated by the presence of the free amino terminal 'propeptide' region of the primary gene product of its normal protein substrates. A series of analogs of the human factor X propeptide have been utilized to demonstrate the structure/function relationships important in these interactions.
Cheung
A
A
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison 53706.
Suttie
J W
JW
Bernatowicz
M
M
D000595:Amino Acid Sequence; D000818:Animals; D019735:Carbon-Carbon Ligases; D004789:Enzyme Activation / Q000187:drug effects; D005170:Factor X / Q000494:pharmacology*; D008025:Ligases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008862:Microsomes, Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000494:pharmacology*; D011498:Protein Precursors / Q000494:pharmacology*; D051381:Rats; D013329:Structure-Activity Relationship
D010446:Peptide Fragments; D011498:Protein Precursors; D005170:Factor X; D008025:Ligases; D019735:Carbon-Carbon Ligases; C020660:glutamyl carboxylase