Exon 2 DNA sequence of the HLA-DRw13b allele obtained from genomes of five different individuals.
Exon 2 nucleotide sequence of the DRB1 gene encoding the HLA-DRw13b allele defined by DNA-RFLP (Restriction Fragment Length Polymorphism) typing, has been obtained by using five heterozygous individuals genomic DNA and a non isotopic automated "dideoxi" methodology. Its comparison with other known homologous DRB1 sequences suggests that two different mechanisms which generate HLA allele variability may have occurred in this particular exon 2: a gene conversion between DRw11 or DRw13 as acceptors and DR4-Dw15 or DRw8.1 as donors and in addition, a non-conservative point mutation at base 221. The relationship between this HLA sequence characteristics and certain diseases susceptibility is discussed.
Corell
A
A
Department of Immunology, Hospital Doce de Octubre, Madrid, Spain.
Martin-Villa
J M
JM
Varela
P
P
Vicario
J L
JL
Martinez-Laso
J
J
Arnaiz-Villena
A
A
D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D005091:Exons / Q000502:physiology*; D015894:Genome, Human; D006684:HLA-DR Antigens / Q000235:genetics*; D059810:HLA-DR Serological Subtypes; D006801:Humans; D008969:Molecular Sequence Data
D006684:HLA-DR Antigens; D059810:HLA-DR Serological Subtypes; C097337:HLA-DR13 antigen; D004247:DNA