Changes in dietary lipid saturation modify fatty acid composition and high-density-lipoprotein binding of adipocyte plasma membrane.
Binding of rat high-density lipoprotein (HDL) to adipocyte plasma membranes appears to be mediated by specific protein-recognition sites, but membrane lipids may also affect these interactions. To study the effect of dietary fatty acid composition on adipocyte-membrane phospholipid composition and HDL binding, male Wistar rats (250 +/- 10 g) were fed diets high in polyunsaturated (20% wt:wt sunflowerseed oil) or saturated and monounsaturated fatty acids (20% lard, by weight). Sunflower-oil vs lard feeding significantly increased the polyunsaturated fatty acid content of membrane diacylglycerophospholipids and the (22:0 and 24:0) content of long-chain fatty acids of sphingomyelin (p less than 0.05) from epididymal and perirenal adipocyte plasma membranes. Concomitant with these changes in membrane phospholipid composition, sunflower-oil vs lard treatment significantly increased the maximum binding capacity (Bmax) of 125I-labeled HDL2 (p less than 0.05). These results show that feeding polyunsaturated fatty acids enhanced HDL2 Bmax of adipocyte plasma membranes, possibly as a consequence of altering the fatty acid composition of membrane phospholipids.
Zsigmond
E
E
Department of Medicine, Faculty of Medicine, University of Toronto, Ontario, Canada.
Parrish
C
C
Fong
B
B
Angel
A
A
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D004041:Dietary Fats / Q000008:administration & dosage / Q000494:pharmacology*; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage / Q000494:pharmacology*; D008075:Lipoproteins, HDL / Q000032:analysis / Q000378:metabolism*; D008297:Male; D008563:Membrane Lipids / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains
D004041:Dietary Fats; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D008075:Lipoproteins, HDL; D008563:Membrane Lipids; D010743:Phospholipids