Sperm morphological analysis: comparison of two methods in human semen.
Sperm morphological analysis was performed by 2 methods: light microscopy on Papanicolaou stained material and scanning electron microscopy. Both methods were found to have acceptably low levels of observer error and a positive correlation existed between them. Sperm morphology was analysed by both methods in 2 groups: (1) 104 proven "fertile" men, husbands of women who had just given birth and whose paternity had been proven by HLA studies with greater than 95.5% certainty; (2) 53 infertile men from primary infertile marriages in whom no female cause could be found and excluding those with azoospermia. There was no statistically significant difference in medians between the 2 groups when sperm morphology was analysed by light microscopy, but analysis by scanning electron microscopy did show a significant difference.
Badenoch
D F
DF
Department of Urology, London Hospital.
Evans
P R
PR
Gray
A
A
Evans
S J
SJ
D006801:Humans; D007248:Infertility, Male / Q000473:pathology; D008297:Male; D008853:Microscopy / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods; D013094:Spermatozoa / Q000166:cytology* / Q000648:ultrastructure