title: Repetition structure of mammalian nuclear DNA.
abstract: We have used Fragmentation Sequencing logic to analyse the repetition structure of several large human genomic genes. The method, based on a proposed laboratory scheme for DNA sequencing, detects short sequences which are repeated near, but not necessarily adjacent, to each other (cryptically simple DNA). We find a low frequency of such repeats. There is a slight excess of such repeats in introns over exons, and a slight but significant excess in genomic DNA over random DNA, confirming that cryptically simple sequences are over-represented in the genome. The analysis suggests that Fragmentation Sequencing will be a suitable method for sequencing large mammalian genes.
authors.affiliation: Department of Biochemistry, University of Bath, Claverton Down, U.K.
authors.forename: F
authors.initials: F
authors.lastname: Smillie
authors.forename: W
authors.initials: W
authors.lastname: Bains
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA; D005340:Fibrinogen / Q000235:genetics; D015894:Genome, Human; D006801:Humans; D008322:Mammals / Q000235:genetics; D008957:Models, Genetic; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*
chemical_list: D005340:Fibrinogen; D004247:DNA