Phosphorylation of ornithine decarboxylase in intact erythroleukemia cells.
32P-labeled ornithine decarboxylase was isolated by immunoprecipitation from murine erythroleukemia cells incubated in a medium containing [32P]ortophosphoric acid. Analysis of immunoprecipitate by SDS-polyacrylamide gel electrophoresis and autoradiography revealed a radiolabeled band, which corresponded to the position of mouse ornithine decarboxylase, phosphorylated in vitro by casein kinase-2. A preparation of casein kinase-2 purified from nuclei of erythroleukemia cells could also phosphorylate mouse ornithine decarboxylase.
Flamigni
F
F
Dipartimento di Biochimica, Università di Bologna, Italy.
Marmiroli
S
S
Meggio
F
F
Guarnieri
C
C
Pinna
L A
LA
D000818:Animals; D047388:Casein Kinases; D002467:Cell Nucleus / Q000201:enzymology; D004915:Leukemia, Erythroblastic, Acute / Q000201:enzymology*; D051379:Mice; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D010766:Phosphorylation; D011233:Precipitin Tests; D011494:Protein Kinases / Q000378:metabolism; D011817:Rabbits; D014407:Tumor Cells, Cultured
D011494:Protein Kinases; D047388:Casein Kinases; D009955:Ornithine Decarboxylase