Characterization of allergenic components in sap extract from the weeping fig (Ficus benjamina).
The allergen composition of crude extract from sap (latex) of the weeping fig (Ficus benjamina) was investigated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and immunoblotting. The allergenic components were detected by sera from 11 occupationally exposed plant keepers, of whom 7 were non-atopic, and from 9 non-occupationally exposed atopic patients with a positive radio-allergosorbent test to weeping fig. The allergen-antibody complexes were visualized by rabbit anti-IgE and beta-galactosidase-labelled sheep anti-rabbit IgG, using a chromogenic insoluble substrate. A total of 11 allergenic components were identified. Three of them were found to be major allergenic components, identified by more than 50% of the investigated sera. These 3 IgE-binding components had molecular weights of approximately 29,000, 28,000 and 25,000 daltons, respectively. The major allergenic components were denatured by heat in the temperature range of 60-90 degrees C.
Axelsson
I G
IG
Department of Clinical Immunology, Karolinska Hospital, Stockholm, Sweden.
Johansson
S G
SG
Larsson
P H
PH
Zetterström
O
O
D000328:Adult; D000485:Allergens / Q000276:immunology / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000209:etiology; D015151:Immunoblotting; D007073:Immunoglobulin E / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D010936:Plant Extracts / Q000032:analysis* / Q000276:immunology
D000485:Allergens; D010936:Plant Extracts; D007073:Immunoglobulin E