title: Simple and rapid purification of brevin.
abstract: Brevin or plasma gelsolin, a calcium dependent actin-binding and actin-severing protein, was purified from bovine plasma by a very rapid and simple procedure; ammonium sulfate fractionation and only one step of anion exchange column chromatography by a convenient use. It takes only 24 hrs to complete all the procedure. The purity of brevin prepared by this method was more than 95% on SDS-PAGE and total recovery was much better than previous preparation methods. This brevin preparation has about 8 isomers on 2-D PAGE and strong severing activity on F-actin under electron microscopic observation.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, University of Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kurokawa
authors.forename: W
authors.initials: W
authors.lastname: Fujii
authors.forename: K
authors.initials: K
authors.lastname: Ohmi
authors.forename: T
authors.initials: T
authors.lastname: Sakurai
authors.forename: Y
authors.initials: Y
authors.lastname: Nonomura
mesh_terms: D000199:Actins / Q000648:ultrastructure; D000645:Ammonium Sulfate; D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange; D005069:Evaluation Studies as Topic; D018260:Gelsolin; D007526:Isoelectric Point; D007536:Isomerism; D008840:Microfilament Proteins / Q000097:blood / Q000302:isolation & purification* / Q000648:ultrastructure
chemical_list: D000199:Actins; D018260:Gelsolin; D008840:Microfilament Proteins; C016953:brevin; D000645:Ammonium Sulfate