Effects of various lysosphingolipids on cell growth, morphology and lipid composition in three neuroblastoma cell lines.
The cell numbers of three mouse neuroblastoma cell lines were decreased upon incubation with lysosphingolipids in the following order of effectiveness: lysosulfatide (lysoCS) greater than psychosine (Ps) greater than sphingosylphosphocholine (SPC). The different cell lines showed characteristic sensitivities to various concentrations of lysolipids less than 150 microM. Interestingly, only SPC induced neurite outgrowth and changed the lipid composition, modifying the amounts of cholesterol, sphingomyelin (SM) and ganglioside GM3 in all cell lines. The effect of SPC on these cell lines was comparable to the effect of N-acetyl SPC (NAcSPC) rather than that of SM.
Sugiyama
E
E
Department of Lipid Biochemistry, Shinshu University School of Medicine, Matsumoto, Japan.
Uemura
K
K
Hara
A
A
Taketomi
T
T
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002794:Choline / Q000031:analogs & derivatives*; D005732:Gangliosides / Q000378:metabolism; D007700:Kinetics; D050356:Lipid Metabolism; D051379:Mice; D009447:Neuroblastoma; D010743:Phospholipids / Q000378:metabolism; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000494:pharmacology; D011609:Psychosine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
D005732:Gangliosides; D010743:Phospholipids; C005356:sphingosine phosphorylcholine; D010767:Phosphorylcholine; D011609:Psychosine; C019064:psychosine-3'-sulfate ester; D002794:Choline; D013110:Sphingosine