High-performance liquid chromatographic determination of isepamicin in plasma, urine and dialysate.
A high-performance liquid chromatographic method for the measurement of isepamicin, a new aminoglycoside, in plasma, urine and dialysate is reported. The assay utilizes a simple extraction of isepamicin in plasma using commercially available Cyano solid-phase cartridges and dilution of urine and dialysate samples. The separation is performed on a Hypersil C18 column (15 cm X 4.6 mm I.D., 5 microns particle size) and utilizes a mobile phase consisting of 10% methanol and 90% buffer solution containing 0.01 M sodium hexanesulfonate, 0.1 M sodium sulfate and 17 mM acetic acid. The flow-rate is 1.1 ml/min. Dibekacin is used as the internal standard. Isepamicin is derivatized post-column with o-phthalaldehyde for spectrofluorometric detection. The method can also be used for the measurement of other aminoglycosides, i.e. tobramycin, kanamycin, netilmicin and gentamicin. The assay is fast, accurate and has a quantitation limit of 100 ng/ml isepamicin in plasma and 50 ng/ml in urine and dialysate.
Maloney
J A
JA
Drug Evaluation Unit, Hennepin County Medical Center, Minneapolis, MN 55415.
Awni
W M
WM
D000617:Aminoglycosides / Q000032:analysis; D000704:Analysis of Variance; D002021:Buffers; D002851:Chromatography, High Pressure Liquid; D015314:Dialysis Solutions / Q000032:analysis*; D005839:Gentamicins / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D013050:Spectrometry, Fluorescence
D000617:Aminoglycosides; D002021:Buffers; D015314:Dialysis Solutions; D005839:Gentamicins; C016927:isepamicin