Acetobacter cellulose pellicle as a temporary skin substitute.
A bacterial strain with morphological and biochemical properties close to Acetobacter xylinum has been cultured in nonagitated, inverted sucrose- and yeast water-based medium for the production of thick, smooth, and floating cellulosic pellicles. The cellulose content (greater than 90%, dry weight, depending on the efficiency of water washing) and the beta-D-homopolyglucan nature of these pellicles were assessed by physical, chemical, and enzymatic methods. The apyrogenic bacterial biomass, a minor component of the dried biofilm (BioFill), is inactivated by ethylene dioxide. Once applied on exudating or bloody tissues, this biofilm displays several advantages as a biological dressing, and hence, it is valuable as a temporary skin substitute in the treatment of skin wounds, such as burns, ulcers, grafts, and as an adjuvant in dermal abrasions.
Fontana
J D
JD
LQBB-Biomass Chemio/Biotechnology Lab, Department of Biochemistry, UFPR, Curitiba-PR, Brazil.
de Souza
A M
AM
Fontana
C K
CK
Torriani
I L
IL
Moreschi
J C
JC
Gallotti
B J
BJ
de Souza
S J
SJ
Narcisco
G P
GP
Bichara
J A
JA
Farah
L F
LF
D000091:Acetobacter / Q000378:metabolism; D001458:Bandages; D001684:Biological Dressings; D002482:Cellulose / Q000096:biosynthesis / Q000627:therapeutic use*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D012867:Skin; D014961:X-Ray Diffraction
D002482:Cellulose