title: The role of fibrinogen in staphylococcal adherence to catheters in vitro.
abstract: A study was conducted to examine the factors involved in adherence of Staphylococcus aureus to various catheter materials. Staphylococcal adherence to catheters coated with fibrinogen was specific and significantly increased compared with that to control catheters (preincubated in phosphate-buffered saline). The adherence of S. aureus to fibrinogen-coated catheters was blocked by goat anti-human fibrinogen antibody, but not fibronectin, vitronectin, or nonimmune goat IgG. Adherence to catheter tubings coated with fibrin did not differ markedly from that to those coated with fibrinogen alone, suggesting the conservation of the staphylococcal binding domain during the conversion of fibrinogen to fibrin. Plasma proteins such as fibronectin or factor XIII that interacted with fibrin did not modify staphylococcal adherence to fibrin. Adherence studies with staphylococcal cells treated with proteinases revealed that staphylococcal surface protein(s) were responsible for fibrinogen binding. These data indicate that fibrinogen or fibrin is the dominant plasma mediator of staphylococcal adherence to intravascular catheters.
authors.affiliation: Laboratory of Bacteriology and Immunology, Rockefeller University, New York, NY 10021.
authors.forename: A L
authors.initials: AL
authors.lastname: Cheung
authors.forename: V A
authors.initials: VA
authors.lastname: Fischetti
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology*; D001426:Bacterial Proteins / Q000032:analysis; D001798:Blood Proteins / Q000502:physiology; D002408:Catheters, Indwelling; D004797:Enzyme-Linked Immunosorbent Assay; D005337:Fibrin / Q000502:physiology; D005340:Fibrinogen / Q000502:physiology*; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D011095:Polyethylenes; D011140:Polyurethanes; D013211:Staphylococcus aureus / Q000378:metabolism* / Q000648:ultrastructure; D013997:Time Factors; D014520:Urethane
chemical_list: D001426:Bacterial Proteins; D001798:Blood Proteins; D008565:Membrane Proteins; D011095:Polyethylenes; D011140:Polyurethanes; D014520:Urethane; D005337:Fibrin; D005340:Fibrinogen