A sensitive high performance liquid chromatography microassay for bemoradan, a novel cardiotonic agent, in plasma/serum using a semi-automatic liquid/solid extraction sample preparation system--AASP.
A high performance liquid chromatographic method for the measurement of bemoradan levels in plasma/serum is described. This method uses Varian's AASP (Varian Associates, Sunnyvale, CA, USA), a semi-automatic liquid/solid extraction sample preparation system. It requires only small volumes of plasma/serum samples (0.2-1 mL) and needs no organic solvent for sample preparation. The mean recovery of bemoradan at plasma or serum concentrations of 0.5-100 ng/mL is 82%. The assay has a detection limit of 0.5 ng/mL (when 1 mL of plasma/serum is used) and is linear in the concentration range 0.5-500 ng/mL.
Huang
S M
SM
Drug Metabolism Division, R.W. Johnson Pharmaceutical Research Institute, Ortho Pharmaceutical Corporation, Raritan, NJ 08869.
Chien
S C
SC
Flor
S C
SC
D000818:Animals; D001322:Autoanalysis; D048588:Benzoxazines; D002316:Cardiotonic Agents; D002851:Chromatography, High Pressure Liquid / Q000706:statistics & numerical data; D004285:Dogs; D006801:Humans; D000432:Methanol; D008832:Microchemistry; D010078:Oxazines / Q000097:blood*; D010710:Phosphates; D011724:Pyridazines / Q000097:blood*; D011786:Quality Control; D012997:Solvents
D048588:Benzoxazines; D002316:Cardiotonic Agents; D010078:Oxazines; D010710:Phosphates; D011724:Pyridazines; D012997:Solvents; C062457:bemoradan; C018279:sodium phosphate; D000432:Methanol