Production of carrier-free 66Ga and labeling of antimyosin antibody for positron imaging of acute myocardial infarction.
A method for labeling monoclonal antimyosin antibodies with 66Ga is described. The radionuclide is a positron emitter (t1/2: 9.4h) produced by means of the 63Cu (4He, n) 66Ga reaction. Purification of the 66Ga from the copper target is described in detail. Monoclonal antimyosin antibodies are labeled with 66Ga at a high yield (99%) by transcomplexation from an acetate buffer with the diethylenetriamine pentaacetic acid (DTPA)-labeled antibody and preliminary evaluated in two dogs for imaging of myocardial necrosis by positron emission tomography.
Goethals
P
P
Laboratory of Analytical Chemistry, Faculty of Pharmaceutical Sciences, University Hospital, Gent, Belgium.
Coene
M
M
Slegers
G
G
Vogelaers
D
D
Everaert
J
J
Lemahieu
I
I
Colardyn
F
F
Heyndrickx
G R
GR
D000818:Animals; D000911:Antibodies, Monoclonal; D004285:Dogs; D005710:Gallium Radioisotopes; D007553:Isotope Labeling; D009203:Myocardial Infarction / Q000000981:diagnostic imaging*; D009218:Myosins / Q000276:immunology*; D004369:Pentetic Acid; D011876:Radionuclide Generators; D014055:Tomography, Emission-Computed
D000911:Antibodies, Monoclonal; D005710:Gallium Radioisotopes; D004369:Pentetic Acid; D009218:Myosins