The order of hepatic cytotoxicity of bile salts in vitro does not agree with that examined in vivo in rats.
Using an enzyme release from isolated rat hepatocytes incubated with a bile salt as a marker, the cytotoxic order of bile salts was found to be taurochenodeoxycholate, glycochenodeoxycholate greater than tauroursodeoxycholate, glycoursodeoxycholate, cholate greater than taurocholate, glycocholate. Thus, the cytotoxicity of conjugates of ursodeoxycholate was greater than that of conjugates of cholate. However, these data do not agree with the order of cytotoxicity of these bile salts previously studied in vivo by the authors which demonstrated the least cytotoxic nature of conjugates of ursodeoxycholate.
Ohta
M
M
First Laboratory of Clinical Physiology, Tokyo Metropolitan Institute of Gerontology, Japan.
Kanai
S
S
Kitani
K
K
D015720:5'-Nucleotidase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D001646:Bile / Q000201:enzymology; D001647:Bile Acids and Salts / Q000633:toxicity*; D001711:Biotransformation; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014166:Transferases / Q000097:blood
D001647:Bile Acids and Salts; D007770:L-Lactate Dehydrogenase; D014166:Transferases; D000469:Alkaline Phosphatase; D015720:5'-Nucleotidase