[Statement: alkaline phosphatases. Genetic aspects. Identification and clinical interpretation].
The human alkaline phosphatases constitute a family of several isoenzyme forms. They are coded by 3 different structural genes. The authors find respectively the placental isoenzymes, the intestinal isoenzymes, and the isoenzymes coded by a gene called aspecific tissular. These last isoenzymes coming from the liver and the bone tissue have very similar structures leading to a very difficult differentiation and quantification. In this study, a summary is made of the main procedures at the disposal of biologist, which allows to have a differentiation of liver and bone fractions. The selected technique proposes the reactivity by affinity with a lectin. Owing to its excellent resolution, this method must contribute to a better follow-up of bone and liver metastasis and to the detection of intestinal isoenzymes, allowing the research of a possible link with different pathologies.
Favre
G
G
Centre Claudius-Regaud, Toulouse.
Canal
P
P
Soula
G
G
Bagnoux
J P
JP
Jardillier
J C
JC
D000469:Alkaline Phosphatase / Q000235:genetics / Q000302:isolation & purification*; D001859:Bone Neoplasms / Q000175:diagnosis / Q000556:secondary; D004796:Clinical Enzyme Tests; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007527:Isoenzymes / Q000302:isolation & purification; D008113:Liver Neoplasms / Q000175:diagnosis / Q000556:secondary
D007527:Isoenzymes; D000469:Alkaline Phosphatase
Communiqué: les phosphatases alcalines. Aspect génétique. Identification et interprétation clinique.