Excitation and inhibition of the heart of the snail, Lymnaea, by non-FMRFamidergic motoneurons.
1. The present paper extends the model of neuronal control of the Lymnaea heart by the use of intracellular recording techniques to identify further types of cardioactive neurons in the CNS that, like the previously described E heart excitor (Ehe) cells, influence the myogenic heartbeat. 2. Four new types of neuron that act on the heart are described. These are excitatory Hhe and She cells (H and S heart excitors) and the inhibitory Khi cell (K heart inhibitor). The fourth class, tonus pericardium excitor (Tpe), modulates the heart by action on pericardial tissue. 3. Pharmacologic, electrophysiological, and anatomic evidence is presented that shows that these cells are motoneurons, innervating heart muscle fibers directly: blocking central chemical synapses failed to prevent the actions of the neurons on the heart; simultaneous intracellular recordings showed unitary EJPs in heart muscle after 1:1 and with constant delay from evoked neuronal action potentials; intracellular injection of the dye Lucifer yellow showed all cells had axonal branches entering the intestinal nerve (which innervates the heart). 4. The use of selective antagonists to 5-hydroxytryptamine (5-HT) (cinanserin), dopamine (ergonovine), and acetylcholine (alpha-bungarotoxin) provided evidence that the actions of She and Hhe cells are mediated by 5-HT, whereas those of the Khi cell are mediated by acetylcholine. 5. A cyclically active network of three interneuronal inputs acting on the heart motoneurons is described. 6. One of these, input 3, is responsible for periodic excitation of the heart via its effects on the Hhe cells.
Buckett
K J
KJ
MRC Neurophysiology Research Group, School of Biological Sciences, University of Sussex, Falmer, Brighton, United Kingdom.
Peters
M
M
Benjamin
P R
PR
D000109:Acetylcholine / Q000502:physiology; D000818:Animals; D006329:Heart Conduction System / Q000502:physiology*; D008195:Lymnaea / Q000502:physiology*; D008465:Mechanoreceptors / Q000502:physiology; D009046:Motor Neurons / Q000502:physiology*; D009433:Neural Inhibition; D010496:Pericardium / Q000294:innervation; D010812:Physical Stimulation; D012701:Serotonin / Q000502:physiology; D013569:Synapses / Q000502:physiology
D012701:Serotonin; D000109:Acetylcholine