[Structure of the chromatin with long linker DNA].
The method of velocity sedimentation have been used to investigate ionic-strength-induced compaction of sea urchin sperm chromatin characterized by extremely long linker DNA (100 b.p.). The dependence of sedimentation coefficients of oligonucleosomes on the number of nucleosomes in the chain have been studied in the range of ionic strength from 0.005 to 0.085. Analysis of these data indicates that such structural parameters of sea urchin sperm chromatin fibre as the diameter of the chain and the length of the chain per nucleosome are quite similar to those of chromatin with shorter linker DNA, but the DNA packing ratio is higher. The structure of sea urchin sperm oligonucleosomes agrees well with the model of three-dimensional zig-zag-shaped chain with linker DNA forming a loop. The possible role of alpha-helical regions of the C-terminal domain of sea urchin sperm histone H1 in the long linker DNA folding is discussed.
Osipova
T N
TN
Karpova
E V
EV
Konditerov
S V
SV
Vorob'ev
V I
VI
D000818:Animals; D002843:Chromatin / Q000032:analysis* / Q000648:ultrastructure; D004247:DNA / Q000032:analysis* / Q000648:ultrastructure; D004587:Electrophoresis, Agar Gel; D008297:Male; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000032:analysis / Q000648:ultrastructure; D009994:Osmolar Concentration; D012617:Sea Urchins; D013094:Spermatozoa / Q000032:analysis
D002843:Chromatin; D009707:Nucleosomes; D004247:DNA
Struktura khromatina s dlinnoĭ linkernoĭ DNK.