Structural characterization of canine PYY.
PYY was purified from canine colonic mucosa by sequential steps of reverse phase HPLC and ion-exchange FPLC. Microsequence, amino acid and mass spectral analyses of the purified peptide and its tryptic fragments were consistent with the structure: YPAKPEAPGEDASPEELSRYYASLRHYLNLVTRQRY-amide. Canine PYY(1-36) has the identical sequence as porcine and rat PYY but differs from human PYY at position 3, with Ala instead of Ile, and position 18, with Ser instead of Asn. A smaller form, PYY(3-36), was also purified and characterized. It may differ in its biological activity from the intact peptide and could act as a partial antagonist or agonist of PYY(1-36).
Eysselein
V E
VE
Harbor-UCLA Medical Center, Department of Gastroenterology, Torrance 90509.
Eberlein
G A
GA
Grandt
D
D
Schaeffer
M
M
Zehres
B
B
Behn
U
U
Schaefer
D
D
Goebell
H
H
Davis
M
M
Lee
T D
TD
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003106:Colon / Q000032:analysis; D004285:Dogs; D005768:Gastrointestinal Hormones / Q000302:isolation & purification; D006801:Humans; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010446:Peptide Fragments; D019894:Peptide YY; D010455:Peptides / Q000302:isolation & purification; D011863:Radioimmunoassay; D051381:Rats; D013045:Species Specificity; D013552:Swine; D014357:Trypsin
D000596:Amino Acids; D005768:Gastrointestinal Hormones; D010446:Peptide Fragments; D010455:Peptides; D019894:Peptide YY; C061785:peptide YY (3-36); D014357:Trypsin