Integration of human immunodeficiency virus type 1 DNA in vitro.
A highly efficient cell-free system for the integration of human immunodeficiency virus type 1 DNA is described. Linear viral DNA synthesis occurs in the cytoplasm of newly infected cells, reaching peak levels 4 hr after infection. The linear viral DNA molecules present in cytoplasmic extracts are capable of integrating into heterologous DNA targets in vitro. The viral DNA resides in a high molecular weight nucleoprotein structure that can be separated from the bulk of cellular protein and nucleic acid without a detectable decrease in the ability to integrate in vitro.
Farnet
C M
CM
Dana-Farber Cancer Institute, Department of Pathology, Harvard Medical School, Boston, MA 02115.
Haseltine
W A
WA
D015139:Blotting, Southern; D002460:Cell Line; D002474:Cell-Free System; D003593:Cytoplasm / Q000382:microbiology; D004247:DNA / Q000378:metabolism*; D004279:DNA, Viral / Q000096:biosynthesis / Q000378:metabolism*; D003852:Deoxyribonucleoproteins / Q000378:metabolism; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D066298:In Vitro Techniques; D011995:Recombination, Genetic; D013997:Time Factors; D014779:Virus Replication
D004279:DNA, Viral; D003852:Deoxyribonucleoproteins; D004247:DNA