Patterns of expression of tumor markers on non-transformed human mammary epithelial cells cultured in vitro.
We have examined the expression of 7 well defined tumor markers/tumor associated antigens (H type 2, X, Y, sialyl-Lea, CEA, MAM-6, and Tn) and a tumor associated antigen defined by a new own monoclonal antibody on non-transformed human epithelial cell lines derived from reduction mammoplasties by means of immunocytochemistry with monoclonal antibodies. Two cell types are discernible: slowly or non-proliferating, lumenal derived (I), and proliferating, stem cell-like, basal cell-derived cells (II). Five out of the 8 tumor markers were expressed on type I cells, and all 8 on type II cells. The number of positive cells varied considerably from a few to 100 per cent depending on the individual markers. The observed patterns proved to be characteristic and reproducible; they appear to reflect the developmental stage of the cells cultured in vitro rather than a direct influence of the culture conditions.
Karsten
U
U
Central Institute of Molecular Biology, Academy of Sciences of the GDR, Robert Rössle Institute, Berlin-Buch.
Papsdorf
G
G
Pauly
A
A
Stosiek
P
P
Kasper
M
M
Pasternak
G
G
D014408:Biomarkers, Tumor / Q000032:analysis*; D001940:Breast / Q000032:analysis* / Q000276:immunology; D002272:Carcinoembryonic Antigen / Q000032:analysis; D002460:Cell Line; D006801:Humans; D066298:In Vitro Techniques; D008562:Membrane Glycoproteins / Q000032:analysis; D018396:Mucin-1; D012016:Reference Values
D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D008562:Membrane Glycoproteins; D018396:Mucin-1