title: A novel T-cell protein which recognizes a palindromic sequence in the negative regulatory element of the human immunodeficiency virus long terminal repeat.
abstract: Two major protein-binding sites within the negative regulatory element of the human immunodeficiency virus type 1 long terminal repeat have been identified. One (site B) contained a palindromic sequence with homology to steroid/thyroid hormone response elements but was distinct from previously described binding sites of this class. A novel T-cell protein recognized the palindromic sequence within site B and also bound estrogen- or thyroid hormone-response elements with lower affinity. A 7-base-pair mutation in the site B palindrome, which destroyed protein binding, resulted in increased expression from the human immunodeficiency virus type 1 long terminal repeat in T cells.
authors.affiliation: Institute of Cancer Research, Chester Beatty Laboratories, London, England.
authors.forename: K
authors.initials: K
authors.lastname: Orchard
authors.forename: N
authors.initials: N
authors.lastname: Perkins
authors.forename: C
authors.initials: C
authors.lastname: Chapman
authors.forename: J
authors.initials: J
authors.lastname: Harris
authors.forename: V
authors.initials: V
authors.lastname: Emery
authors.forename: G
authors.initials: G
authors.lastname: Goodwin
authors.forename: D
authors.initials: D
authors.lastname: Latchman
authors.forename: M
authors.initials: M
authors.lastname: Collins
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000502:physiology*; D015967:Gene Expression Regulation, Viral; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D066298:In Vitro Techniques; D008745:Methylation; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000502:physiology*; D012045:Regulatory Sequences, Nucleic Acid; D012091:Repetitive Sequences, Nucleic Acid; D012097:Repressor Proteins / Q000502:physiology; D013601:T-Lymphocytes / Q000382:microbiology*
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D012097:Repressor Proteins