Reduction-mediated technetium-99m labeling of monoclonal antibodies.
A simple and generally applicable method for labeling antibodies with technetium-99m (99mTc) is described. Following reduction of intrinsic disulphide bonds, the antibody is labeled with 99mTc in the presence of a weak competing ligand methylene diphosphonate. High labeling efficiencies (greater than 97%), in a final labeling step taking only a few minutes, can be routinely obtained with high in-vitro stability over 24 hr. No effect upon antibody reactivity is seen.
Mather
S J
SJ
Department of Nuclear Medicine, St. Bartholomews Hospital, London, United Kingdom.
Ellison
D
D
D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics; D007553:Isotope Labeling / Q000379:methods*; D051379:Mice; D013667:Technetium; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D013667:Technetium