rUBF, an RNA polymerase I transcription factor from rats, produces DNase I footprints identical to those produced by xUBF, its homolog from frogs.
Rat cells contain a DNA-binding polymerase I transcription factor, rUBF, with properties similar to UBF homologs that have been purified from both human (hUBF) and frog (xUBF) cells. In this note we report the affinity purification of rUBF to apparent homogeneity and show that UBFs from both rat and frog have identical footprinting characteristics on templates from either species. Furthermore, xUBF was able to stimulate transcription from rat RNA polymerase I promoters in a partially fractionated rat extract that was UBF dependent. These results strengthen the conclusion that all vertebrate cells contain a UBF homolog whose DNA-binding specificity and function have been strongly conserved.
Pikaard
C S
CS
Hutchinson Cancer Research Center, Seattle, Washington 98104.
Smith
S D
SD
Reeder
R H
RH
Rothblum
L
L
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism*; D009710:Nucleotide Mapping; D035463:Pol1 Transcription Initiation Complex Proteins; D011401:Promoter Regions, Genetic; D012318:RNA Polymerase I / Q000235:genetics*; D051381:Rats; D014157:Transcription Factors / Q000302:isolation & purification / Q000378:metabolism*; D014982:Xenopus laevis
D004268:DNA-Binding Proteins; D035463:Pol1 Transcription Initiation Complex Proteins; D014157:Transcription Factors; C063844:transcription factor UBF; D012318:RNA Polymerase I; D003850:Deoxyribonuclease I