[The recording of human fibroblast metabolism in vitro by the chromatographic-mass spectrometric determination of the metabolites excreted].
Metabolites excreted into culture medium by human skin fibroblasts have been studied by high resolution gas chromatography and mass spectrometry. Parameters for 29 metabolites have been obtained and 11 of them have been identified. Excreted metabolites reflect activity of certain metabolic processes in fibroblasts. Comparison of chromatographic and mass spectrometric parameters of cellular metabolites with the metabolites excreted with urine revealed that most metabolites excreted from fibroblasts differ from urine metabolites. The possibility for secondary transformation of cell metabolites in organism and specificity of metabolism in different tissues has been discussed.
Antoshechkin
A G
AG
Tatur
V Iu
VIu
Perevezentseva
O M
OM
Maksimova
L A
LA
D000328:Adult; D002478:Cells, Cultured / Q000032:analysis / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D002849:Chromatography, Gas; D004171:Diploidy; D005260:Female; D005347:Fibroblasts / Q000032:analysis / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008970:Molecular Weight; D012867:Skin / Q000032:analysis / Q000378:metabolism*
Registratsiia metabolizma fibroblastov cheloveka in vitro putem khromato-mass-spektrometricheskogo opredeleniia ékskretiruemykh imi metabolitov.