Lipid A mutants of Vibrio cholerae: isolation and partial characterization.
Vibrio cholerae mutants resistant to common antibiotics and neutral and anionic detergents were isolated. Analysis of isolated outer membranes revealed a significant deficiency in the acylation of lipid A in the resistant strains. The content of amide-linked and ester-bound fatty acids in the lipid A of the mutant strains compared to that of the wild type was about 50-56% and 29-37% respectively. This defect was specific for lipid A as there was no change in the acylation of phospholipids. The reduction in fatty acid content of lipid A was reflected in the altered endotoxic properties in the mutant strains.
Paul
S
S
Biophysics Division, Indian Institute of Chemical Biology, Calcutta, India.
Sen
A K
AK
Banerjee
N
N
Chatterjee
A N
AN
Das
J
J
D004352:Drug Resistance, Microbial; D005227:Fatty Acids / Q000032:analysis; D008050:Lipid A / Q000235:genetics / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000633:toxicity; D009154:Mutation; D014734:Vibrio cholerae / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity
D005227:Fatty Acids; D008050:Lipid A; D008070:Lipopolysaccharides