Interleukin 3 perfusion in W/Wv mice allows the development of macroscopic hematopoietic spleen colonies and restores cutaneous mast cell number.
The genetically anemic W/Wv mice are characterized by the inability of their bone marrow cells to form macroscopic pluripotent hematopoietic colonies in the spleen of irradiated recipients upon transfer (colony-forming units). Furthermore, they almost totally lack mast cells, notably in the skin. In the present study, we have tested the effect of recombinant murine interleukin 3 (rmIL-3) on W/Wv mice hematopoiesis. Transfer of W/Wv bone marrow cells into lethally irradiated recipients perfused with rmIL-3 is followed by the appearance of macroscopic spleen colonies. Moreover, perfusion of rmIL-3 in W/Wv mice: (a) restores almost normal total numbers of hematopoietic precursors (colony-forming cells), but without modification of anemia; and (b) leads to the appearance of a normal number of mastocytes in the skin.
Ody
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Department of Pathology, Centre Médical Universitaire, University of Geneva, Switzerland.
Kindler
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Vassalli
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D000740:Anemia / Q000188:drug therapy / Q000473:pathology; D000818:Animals; D016026:Bone Marrow Transplantation / Q000276:immunology; D002452:Cell Count; D002478:Cells, Cultured; D006410:Hematopoiesis / Q000187:drug effects* / Q000528:radiation effects; D007377:Interleukin-3 / Q000008:administration & dosage / Q000494:pharmacology*; D008407:Mast Cells / Q000187:drug effects* / Q000473:pathology; D051379:Mice; D008817:Mice, Mutant Strains; D010477:Perfusion; D011994:Recombinant Proteins; D013154:Spleen / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects
D007377:Interleukin-3; D011994:Recombinant Proteins