title: The polymerase chain reaction for Mycoplasma pulmonis.
abstract: In vitro DNA amplification by polymerase chain reaction was examined to detect Mycoplasma pulmonis. A pair of synthetic oligonucleotide primers was constructed, and used to amplify a unique sequence of M. pulmonis DNA. Amplified products were detected by agarose gel electrophoresis and verified by blot hybridization with a synthetic oligonucleotide probe. This system detected cellular DNA of M. pulmonis but not M. arthritidis or M. neurolyticum, and thus appears to be useful for M. pulmonis diagnosis.
authors.affiliation: Division of Animal Research, Faculty of Medicine, University of Tokyo.
authors.forename: R
authors.initials: R
authors.lastname: Harasawa
authors.forename: K
authors.initials: K
authors.lastname: Koshimizu
authors.forename: T
authors.initials: T
authors.lastname: Uemori
authors.forename: O
authors.initials: O
authors.lastname: Takeda
authors.forename: K
authors.initials: K
authors.lastname: Asada
authors.forename: I
authors.initials: I
authors.lastname: Kato
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis*; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction
chemical_list: D004269:DNA, Bacterial