Effects of plasmapheresis and autotransfusion on complement recovery in dogs.
To study selectively the dilutional aspects of severe blood loss on the serum complement system, we developed an animal model consisting of isovolumic phlebotomy and reinfusion of washed autologous erythrocytes. This model avoided hypoperfusion, ischemia, and transfusion of foreign antigen. We measured total serum protein, C3 antigen, total complement hemolytic activity, and alternative pathway hemolytic activity. Each of the first three parameters dropped to 55% of initial value (P less than 0.005) by the end of the phlebotomy/reinfusion procedure and returned to normal levels by Day 1 or 2. C3 antigen and total complement hemolytic activity then rose to 150% of normal by Day 4 and gradually returned to normal within 2 weeks. Alternative pathway activity, by contrast, fell by more than 80% (P less than 0.005) within the first 6 hr, recovered by Day 4, and gradually rose to about 140% of normal by Day 21. Trauma patients treated for heavy blood loss who suffer depletion of hemolytic complement during the first few hours may be at greater risk of infection due to immune deficiencies. The implication of the results presented here is that the alternative pathway may be particularly weakened during blood loss and transfusion by simple dilution in addition to the effects of processes omitted in this model. Knowledge of the kinetics of complement recovery, independent of other effects usually accompanying trauma, may be helpful in determining whether these patients might benefit from exogenous manipulation of the complement system.
Ross
M
M
Department of Surgery, Brooklyn Veterans Administration Medical Center, New York.
England
D
D
Webb
H
H
Hoover
E L
EL
D000818:Animals; D001804:Blood Transfusion, Autologous; D003167:Complement Activation; D015932:Complement C3c / Q000032:analysis; D003165:Complement System Proteins / Q000032:analysis*; D004285:Dogs; D008297:Male; D010956:Plasmapheresis
D015932:Complement C3c; D003165:Complement System Proteins