title: Conjugation of a monoclonal antibody with a DTPA modified random copolymer of hydroxyethyl methylacrylate and methyl methacrylate.
abstract: A new approach for covalent coupling diethylenetriaminepentaacetic acid (DTPA) molecules to a partially reduced monoclonal antibody utilizes a malemide modified copolymer of hydroxyethyl methylacrylate and methyl methacrylate (DTPA copolymer) prepared by the group transfer polymerization (GTP) method. An average of 6 DTPA molecules were incorporated per mol maleimeide DTPA copolymer and 1.5 mol maleimide DTPA copolymer per mol antibody. Maleimide DTPA copolymer modified antibody was intramolecularly cross-linked, reduced immunoactivity and had a high in vivo liver uptake.
authors.affiliation: Seragen Inc., Hopkinton, MA 01748.
authors.forename: R D
authors.initials: RD
authors.lastname: Comeau
authors.forename: F A
authors.initials: FA
authors.lastname: Liberatore
authors.forename: A J
authors.initials: AJ
authors.lastname: Cocuzza
authors.forename: G Y
authors.initials: GY
authors.lastname: Emond
authors.forename: I B
authors.initials: IB
authors.lastname: Dicker
mesh_terms: D000179:Acrylates / Q000138:chemical synthesis*; D000818:Animals; D000911:Antibodies, Monoclonal; D007205:Indium Radioisotopes; D007553:Isotope Labeling / Q000379:methods; D008689:Methacrylates / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D051379:Mice; D004369:Pentetic Acid / Q000138:chemical synthesis* / Q000493:pharmacokinetics / Q000031:analogs & derivatives; D014018:Tissue Distribution
chemical_list: D000179:Acrylates; D000911:Antibodies, Monoclonal; C064249:DTPA copolymer; D007205:Indium Radioisotopes; D008689:Methacrylates; D004369:Pentetic Acid