Inhibition of neutrophil and eosinophil chemotactic responses to PAF by the PAF-antagonists WEB-2086, L-652,731, and SRI-63441.
The influence of the three PAF-antagonists WEB-2086, L-652,731, and SRI-63441 on the chemotactic response of neutrophil and eosinophil granulocytes to PAF was investigated. When the PAF-antagonists were added to the cell suspension that was exposed to a gradient of PAF, WEB-2086 and SRI-63441 at the concentration of 10(-6) mol/litre inhibited (P less than .01) the neutrophil and eosinophil chemotactic response to 10(-8) and 10(-9) mol PAF per litre; at the concentration of 5 x 10(-6) mol/litre, WEB-2086 and SRI-63441 also inhibited (P less than .02) the response to 10(-7) mol PAF per litre. Under the same conditions L-652,731 at the concentration of 5 x 10(-6) mol/litre inhibited (P less than .01) the eosinophil chemotactic response to 10(-8) and 10(-9) mol PAF per litre. The inhibition of the chemotactic response to PAF by the three PAF-antagonists was specific, since the chemotactic response to C5f, f-MLP, and LTB4 was not affected by WEB-2086, L-652,731, or SRI-63441, neither was the chemokinetic migration induced by albumin.
Håkansson
L
L
Department of Clinical Chemistry, University Hospital, Uppsala, Sweden.
Venge
P
P
D001381:Azepines / Q000494:pharmacology*; D002633:Chemotaxis / Q000187:drug effects* / Q000502:physiology; D003182:Complement C5 / Q000494:pharmacology; D004804:Eosinophils / Q000187:drug effects / Q000502:physiology*; D005663:Furans / Q000494:pharmacology*; D006801:Humans; D015289:Leukotrienes / Q000494:pharmacology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D011806:Quinolinium Compounds / Q000494:pharmacology*; D014230:Triazoles / Q000494:pharmacology*
D001381:Azepines; D003182:Complement C5; D005663:Furans; D015289:Leukotrienes; D010972:Platelet Activating Factor; D011806:Quinolinium Compounds; D014230:Triazoles; C050531:SRI 63-441; C052518:WEB 2086; D009240:N-Formylmethionine Leucyl-Phenylalanine; C047504:2,5-bis(3,4,5-trimethoxyphenyl)tetrahydrofuran