Accelerated healing of ulcer wounds in the rabbit ear by recombinant human transforming growth factor-beta 1.
A dermal ulcer wound-healing model was established in rabbit ear to examine the effects of recombinant human transforming growth factor-beta 1 (rhTGF-beta 1) in wound healing. Histomorphometric examination of the wounds indicate a biphasic healing response 7 days after a single application of rhTGF-beta 1 at the time of wounding. Statistically significant healing occurred at 5-100 ng but not at higher doses of 500 or 1000 ng rhTGF-beta 1/wound. Enhanced collagen synthesis as determined by [3H]proline incorporation occurred at 15 and 25 ng and was significantly depressed at 500 ng rhTGF-beta 1/wound. Multiple doses of 100 ng rhTGF-beta 1 applied to the wound at the time of wounding and for 3 days after wounding provided results comparable to the single application of growth factor. Delaying treatment 24 hr after wounding did not enhance wound healing compared with vehicle. Our findings suggest that rhTGF-beta 1 can be a valuable growth factor to improve the healing of ulcer wounds.
Beck
L S
LS
Department of Pharmacological Sciences, Genentech Inc., South San Francisco, California 94080.
Chen
T L
TL
Hirabayashi
S E
SE
Deguzman
L
L
Lee
W P
WP
McFatridge
L L
LL
Xu
Y
Y
Bates
R L
RL
Ammann
A J
AJ
D000818:Animals; D003094:Collagen / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D004431:Ear, External / Q000293:injuries; D004848:Epithelium / Q000187:drug effects; D008297:Male; D008938:Mitosis / Q000187:drug effects; D011817:Rabbits; D011994:Recombinant Proteins / Q000627:therapeutic use; D013223:Statistics as Topic; D015291:Transforming Growth Factors / Q000627:therapeutic use*; D014456:Ulcer / Q000188:drug therapy / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects*
D011994:Recombinant Proteins; D015291:Transforming Growth Factors; D003094:Collagen