An endogenous inhibitor of protein kinase C in the pseudopregnant rat ovary.
Removal of contaminating proteins from rat ovarian cytosol by DEAE chromatography results in a 358% recovery of protein kinase C activity. The data suggest that rat ovaries contain an endogenous inhibitor of protein kinase C whose activity dominates that of protein kinase C in the cytosol. The inhibitor is specific for protein kinase C and does not activate the enzyme through proteolysis. This endogenous inhibitor may be important in the hormonal control of protein kinase C in the rat ovary, and may become an important tool in the study of the role of protein kinase C in other cell functions.
Eyster
K M
KM
Department of Physiology and Pharmacology, University of South Dakota, Vermillion 57069.
D000818:Animals; D001921:Brain / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D006706:Homeostasis; D010053:Ovary / Q000201:enzymology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D011555:Pseudopregnancy / Q000201:enzymology*; D051381:Rats
D011493:Protein Kinase C