title: Terminal deoxynucleotidyltransferase containing megadalton complex from young rat thymus nuclei: identification and characterization.
abstract: Nuclear matrix prepared from 2-3 week old rat thymuses contains tightly bound TdT activity which has been quantitatively solubilized with nonionic detergent and sonication. TdT is contained in a discrete complex with a sedimentation value of 23 S. The complex is retained on an anti-TdT antibody column and contains DNA ligase and 3'-5' exonuclease activities as well as DNA and several other proteins but is devoid of replicative DNA polymerases. Such a type of multienzyme complex is absent from the nuclear extracts of thymus prepared from older rats and also from liver and spleen extracts of young and old rats.
authors.affiliation: Biochemistry Division, Bhabha Atomic Research Centre, Bombay, India.
authors.forename: V N
authors.initials: VN
authors.lastname: Pandey
authors.forename: V P
authors.initials: VP
authors.lastname: Dave
authors.forename: M S
authors.initials: MS
authors.lastname: Patil
authors.forename: D S
authors.initials: DS
authors.lastname: Pradhan
authors.forename: S B
authors.initials: SB
authors.lastname: Amrute
authors.forename: M J
authors.initials: MJ
authors.lastname: Modak
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D002467:Cell Nucleus / Q000201:enzymology; D002499:Centrifugation, Density Gradient; D004254:DNA Nucleotidyltransferases / Q000276:immunology / Q000378:metabolism*; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains; D012995:Solubility; D013379:Substrate Specificity; D013950:Thymus Gland / Q000201:enzymology*
chemical_list: D000906:Antibodies; D004254:DNA Nucleotidyltransferases