Adenosine triphosphate compartmentation in the rat heart: a 31P spin-lattice relaxation study.
Longitudinal relaxation times (T1) of phosphorus compounds in the perfused rat heart and erythrocytes were measured using the 31P Driven-Equilibrium Single-Pulse Observation of T1 relaxation (DESPOT) method at 33 degrees C. Both creatine phosphate in the heart and the three phosphate groups of adenosine triphosphate (ATP) in erythrocytes showed single-exponential relaxation. The three phosphate groups of ATP in the heart, however, had two T1 components. The T1 values of the short and the long T1 components of the beta-phosphate of ATP were ca. 0.4 and 14 s, respectively. The fraction with the long T1 represented ca. 30% of the total ATP content. These results suggested that there were two major pools of intracellular ATP in the rat heart which could be determined by 31P NMR spectroscopy.
Suzuki
E
E
Department of Molecular Physiology, National Institute for Physiological Sciences, Aichi.
Maeda
M
M
Kuki
S
S
Steward
M C
MC
Takami
H
H
Seo
Y
Y
Murakami
M
M
Watari
H
H
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002451:Cell Compartmentation; D004912:Erythrocytes / Q000378:metabolism*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D010759:Phosphorus Isotopes; D051381:Rats; D011919:Rats, Inbred Strains
D010759:Phosphorus Isotopes; D000255:Adenosine Triphosphate