Wavelength dependence for UV-induced pyrimidine dimer formation in DNA of human peripheral blood lymphocytes.
An action spectrum for the induction of pyrimidine dimers in human peripheral lymphocytes was determined between 254 and 405 nm. The presence of pyrimidine dimers was determined as UV-induced lesions that were sensitive to the dimer-specific endonuclease from Micrococcus luteus in conjunction with agarose gel electrophoresis. The rate of induction of pyrimidine dimers was maximal at 254 nm. These values can be compared with action spectra for UV-induced in vitro responses of lymphocytes.
Freeman
S E
SE
Lovelace Medical Foundation, Albuquerque, NM 87108.
Ryan
S L
SL
D002470:Cell Survival; D004247:DNA / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004587:Electrophoresis, Agar Gel; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism* / Q000528:radiation effects; D009154:Mutation; D011740:Pyrimidine Dimers / Q000378:metabolism*; D014466:Ultraviolet Rays
D011740:Pyrimidine Dimers; D004247:DNA