Analysis of taurine in feline plasma and whole blood by liquid chromatography with fluorimetric detection and confirmation by thermospray mass spectrometry.
A liquid chromatographic method with fluorimetric detection was developed to measure taurine (2-aminoethanesulfonic acid) in feline plasma and whole blood. Plasma or lysed whole blood was diluted with a mixture of acetonitrile-methanol-triethylamine-water (25:22:3:50, v/v), filtered through a 10,000 dalton exclusion filter and derivatized with dansyl chloride for 30 min at room temperature. Dansyl taurine was separated from other compounds by reversed-phase liquid chromatography using an octadecyl column and a methanol-acetic acid-triethylamine (30:0.5:0.025, v/v) aqueous mobile phase. The effluent was monitored fluorimetrically at an excitation wavelength of 329 nm and an emission wavelength of 530 nm. The presence of mono-dansylated taurine in feline plasma was confirmed by thermospray mass spectrometry. The limit of detection was 16 nmol/ml and the detector response was linear from 40 to 4000 nmol/ml taurine.
Amiss
T J
TJ
Department of Anatomy, College of Veterinary Medicine, North Carolina State University, Raleigh 27606.
Tyczkowska
K L
KL
Aucoin
D P
DP
D000818:Animals; D002415:Cats; D002853:Chromatography, Liquid; D003619:Dansyl Compounds / Q000032:analysis; D013058:Mass Spectrometry / Q000295:instrumentation; D010949:Plasma / Q000032:analysis; D013050:Spectrometry, Fluorescence; D013654:Taurine / Q000097:blood*
D003619:Dansyl Compounds; D013654:Taurine