[The study of complex-formation of DNA with the antimicrobial drug decamethoxine].
The interaction of effective antibacterial drug decametoxyn with natural DNA was studied by UV-spectroscopy. Decametoxyn shows a specificity to nucleotides: it decreases the cooperativity of melting and the thermal stability of DNA parts enriched by AT pairs. The characteristics of the helix-coil transition on the DNA parts enriched by GC-pairs are invariable. Interaction with AT-pairs results in their partial or complete melting at room temperature, followed by intermolecule aggregation. Interacting with phosphates decametoxyn manifests itself not as a dication but as two single-charged ions.
Sorokin
V A
VA
Blagoĭ
Iu P
IuP
Valeev
V A
VA
Gladchenko
G O
GO
Sukhodub
L F
LF
Volianskiĭ
Iu L
IuL
D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D001482:Base Composition; D002417:Cattle; D004247:DNA / Q000187:drug effects* / Q000378:metabolism; D003650:Decamethonium Compounds / Q000378:metabolism / Q000494:pharmacology; D066298:In Vitro Techniques; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D013056:Spectrophotometry, Ultraviolet
D000900:Anti-Bacterial Agents; D003650:Decamethonium Compounds; C000858:decamethoxine; D004247:DNA
Izuchenie kompleksoobrazovaniia DNK s antimikrobnym preparatom dekametoksinom.