Large-scale production of monoclonal antibodies in defined serum-free media in airlift bioreactors.
Forty- and ninety-liter airlift bioreactors have been used successfully to grow hybridoma cell lines in chemically defined serum-free media. In the airlift bioreactor, hybridoma cell growth and monoclonal antibody productivity are comparable to that obtained by conventional cell culture. At sparging rates of 0.60-1.20 vvh (volume of sparged gas per bioreactor volume per hour), the airlift bioreactor achieves rapid mixing and adequate oxygen mass transfer. Foaming is minimal and inconsequential for serum-free media and media supplemented with 5%-10% fetal bovine serum. The use of serum-free medium facilitates monoclonal antibody purification and enhances the purity of the final MAb product.
Petrossian
A
A
Techniclone International, Tustin, CA 92680.
Cortessis
G P
GP
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification; D002478:Cells, Cultured / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D015194:Diffusion Chambers, Culture / Q000295:instrumentation; D006825:Hybridomas / Q000378:metabolism; D051379:Mice; D010100:Oxygen / Q000378:metabolism
D000911:Antibodies, Monoclonal; D003470:Culture Media; D010100:Oxygen