The characterisation of equine interleukin-1.
Equine interleukin-1 has been produced from peripheral blood monocytes by stimulation with E. coli lipopolysaccharide. Sephacryl S200 gel filtration revealed a molecular weight of 17-18 kD. Chromatofocusing of the 17-18 kD peak identified four active fractions. Two major peaks were detected at pH 6.7 and pH 7, with smaller peaks at pH 6.3 and pH 5.9. The pI 7 molecule is probably the equine form of IL-1 beta.
May
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University of Liverpool Veterinary Field Station, Neston, Wirral, Great Britain.
Hooke
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Lees
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D000818:Animals; D002850:Chromatography, Gel / Q000662:veterinary; D006736:Horses / Q000276:immunology*; D006863:Hydrogen-Ion Concentration; D007375:Interleukin-1 / Q000302:isolation & purification*; D007525:Isoelectric Focusing / Q000662:veterinary; D007526:Isoelectric Point; D008070:Lipopolysaccharides / Q000494:pharmacology; D008970:Molecular Weight; D009000:Monocytes / Q000378:metabolism
D007375:Interleukin-1; D008070:Lipopolysaccharides