Solid-phase synthesis of oligodeoxynucleotides containing O6-alkylguanine.
Practical methods for the routine solid-phase synthesis of oligodeoxynucleotides containing O6-alkylguanine are described. It is shown that if the 2-amino group of the alkylated base is protected with a phenylacetyl group, and a mixture of nitrobenzaldoximate ions and ammonia used to remove the protecting groups from the oligomer at the end of the synthesis, there is negligible formation of 2,6-diaminopurine and that after chromatography pure oligomers are obtained.
Smith
C A
CA
Department of Biochemistry, University College and Middlesex School of Medicine, London, UK.
Xu
Y Z
YZ
Swann
P F
PF
D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D002845:Chromatography; D006147:Guanine / Q000031:analogs & derivatives*; D008722:Methods; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000138:chemical synthesis*
D009841:Oligonucleotides; D006147:Guanine; C008449:O-(6)-methylguanine