Purification of 45 KDa estramustine binding protein (EMBP) and preparation of its antibody.
The purification of 45 KDa EMBP and the production of monospecific anti-serum is described. 45 KDa EMBP was purified by relatively simple methods using ion exchange HPLC (TSK-GEL DEAE-5PW column) and size exclusion HPLC (TSK-GEL G3000SW column). The results clearly demonstrated the speed and simplicity of the method using these columns, compared to previously-described methods for purification of 45 KDa EMBP.
Yuasa
H
H
Department of Urology, Gunma University School of Medicine.
Yamanaka
H
H
Imai
K
K
Mashimo
T
T
Kohno
H
H
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000906:Antibodies / Q000032:analysis*; D002352:Carrier Proteins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D029584:Prostatic Secretory Proteins; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains
D000596:Amino Acids; D000906:Antibodies; D002352:Carrier Proteins; D029584:Prostatic Secretory Proteins; C028321:estramustine-binding protein