title: Seasonally occurring lectins from the bryozoan Bugula neritina.
abstract: Two different lectins (termed BnA-I and BnA-II) with distinct carbohydrate specificities were identified and subsequently isolated from the marine bryozoan Bugula neritina. BnA-I hemagglutinating activity was inhibited by N-acetylated hexosamines, their polymers, and glycoproteins rich in these moieties. BnA-II-induced hemagglutination was not blocked by any simple sugars but could be inhibited by several complex glycoproteins (e.g., thyroglobulin and orosomucoid). Both lectins required the presence of Ca(+)+ for reactivity and were purified by affinity chromatographic procedures. Purified BnA-I was determined to have a native molecular weight of 240 Kd and appeared to be a hexameric homopolymer while BnA-II was shown to be a 65-70 Kd monomer. Both lectins showed seasonality in expression, BnA-I appearing in animal extracts prepared in the spring and fall while BnA-II was expressed only during the summer and winter.
authors.affiliation: Biological Sciences Program, S.U.N.Y. College at Old Westbury 11568.
authors.forename: R
authors.initials: R
authors.lastname: Colon-Urban
authors.forename: J D
authors.initials: JD
authors.lastname: Oppenheim
mesh_terms: D000818:Animals; D002013:Bryozoa / Q000032:analysis* / Q000502:physiology; D002241:Carbohydrates / Q000494:pharmacology; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000494:pharmacology; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006595:Hexosamines / Q000494:pharmacology; D037102:Lectins / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000502:physiology; D008970:Molecular Weight
chemical_list: D002241:Carbohydrates; D006023:Glycoproteins; D006595:Hexosamines; D037102:Lectins