Sequences of three VSG mRNAs expressed in a mixed population of Trypanosoma brucei rhodesiense.
A cDNA library was constructed from a mixed population of bloodstream Trypanosoma brucei rhodesiense expressing at least three different VSGs, one of which is immunologically similar to a VSG present during the metacyclic stage. Complete sequence determinations of three full length VSG cDNAs showed that the 5' spliced leader sequence is located 20-26 nucleotides upstream of the start codons of each of the three VSG mRNAs. Two of the VSGs (472 and 487 aa) are members of one C-terminal isotype group while the other, metacyclic-like, VSG (517 aa) is a member of the other C-terminal group. Since VSGs of different sequences have similar three-dimensional structures, comparison of many different VSGs sequences should lead to a more detailed understanding of the relationship between primary and tertiary structures of proteins in general. GenBank accession numbers: M33823, M33824, and M33825.
Reddy
L V
LV
Department of Biochemistry, University of Iowa, Iowa City 52242.
Hall
T
T
Donelson
J E
JE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003545:Cysteine; D015870:Gene Expression; D015723:Gene Library; D006031:Glycosylation; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000235:genetics*; D014346:Trypanosoma brucei brucei / Q000235:genetics* / Q000276:immunology; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000235:genetics*
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D014643:Variant Surface Glycoproteins, Trypanosoma; D003545:Cysteine