title: Use of nonmotile mutants to identify a set of membrane proteins related to gliding motility in Cytophaga johnsonae.
abstract: Nonmotile mutants of the gliding bacterium Cytophaga johnsonae were examined to identify proteins that might be involved in gliding motility. Wild-type and mutant cell proteins were solubilized and fractionated by using Triton X-114, and the proteins that partitioned into the aqueous phase or the detergent phase were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis for proteins that differed between wild-type and mutant cells. Seventeen proteins, ranging in size from 16 to 150 kilodaltons, were implicated by this technique as having some relationship to gliding and were designated Gld-1 through Gld-17. All Gld proteins behaved as integral membrane proteins, partitioning into the detergent phase. All 56 mutants examined exhibited changes in 1 or more of the Gld proteins, with the number of proteins altered in any mutant varying from 1 to 11. Several lines of evidence suggested that proteins Gld-12 through Gld-15 are glycoproteins. Analysis of banding patterns of detergent-fraction proteins of motile revertants supported the idea that the Gld proteins have a role in gliding motility.
authors.affiliation: Department of Bacteriology, University of Wisconsin, Madison 53706.
authors.forename: J L
authors.initials: JL
authors.lastname: Pate
authors.forename: D M
authors.initials: DM
authors.lastname: De Jong
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D003589:Cytophaga / Q000032:analysis* / Q000502:physiology; D003902:Detergents / Q000494:pharmacology; D006031:Glycosylation; D008565:Membrane Proteins / Q000032:analysis*; D008970:Molecular Weight; D009068:Movement; D009154:Mutation; D012394:Rosaniline Dyes
chemical_list: D001426:Bacterial Proteins; D003902:Detergents; D008565:Membrane Proteins; D012394:Rosaniline Dyes; C048139:Coomassie blue