Iron-supplemented bovine serum as an alternative to fetal bovine serum in the CHO/HGPRT mutation assay.
Iron-supplemented bovine calf serum (ICS) was found to be a viable alternative to fetal bovine serum (FBS) in the growth promotion and cloning efficiency of Chinese hamster ovary (CHO) cells that are used in the HGPRT mutation assay. Suspension cultures of CHO cells had an average generation time of 11.5 h in ICS and 13.6 h for cells maintained in FBS. This slight difference was due to lot variability on the part of FBS and could be eliminated by routine quality control measures. The average cloning efficiencies for CHO cells cloned in either ICS or FBS were 107% and 88%, respectively, and these values were not statistically different. No appreciable difference was noted in the spontaneous mutation rates of cells cloned in either ICS or FBS. Furthermore, the use of ICS in mutagenicity studies with genotoxic agents shows the serum to be at least equal or superior to FBS in the detection of both direct-acting mutagens and promutagens. These data suggest that ICS is an appropriate serum to be used in the CHO/HGPRT test system. Since ICS is more readily available and considerably less costly than FBS, a substantial reduction in the cost of the assay can be realized.
Oberly
T J
TJ
Toxicology Division, Lilly Research Laboratories, Eli Lilly and Company, Greenfield, IN 46140.
Rexroat
M A
MA
Richardson
K K
KK
D000818:Animals; D002417:Cattle; D002455:Cell Division; D002478:Cells, Cultured; D002999:Clone Cells; D006224:Cricetinae; D003470:Culture Media; D005312:Fetal Blood; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D007501:Iron; D009152:Mutagenicity Tests
D003470:Culture Media; D007501:Iron; D007041:Hypoxanthine Phosphoribosyltransferase