Selective cloning of hybridoma cells for enhanced immunoglobulin production using flow cytometric cell sorting and automated laser nephelometry.
Techniques for selective cloning of murine hybridoma cells by flow cytometric cell sorting and use of automated laser nephelometry to determine the resultant clones' immunoglobulin secretion levels are described. Using a commercially available attachment to a fluorescence-activated cell sorter, individual hybridoma cells were successfully distributed into microtiter wells in an automated manner based on their forward angle light scatter properties and their reaction to fluorescein-conjugated anti-mouse-IgG. The techniques were used to estimate successfully the frequency of immunoglobulin-secreting cells in established cultures. In addition, heterogeneity of cell surface immunoglobulin expression was observed and utilized as a criterion for flow sorting of new hybridoma variants. In these studies, clones derived from high (anti-IgG) intensity sorting regions yielded cultures with enhanced immunoglobulin secretion levels, as determined by automated laser nephelometry. Furthermore, the surface immunoglobulin phenotype of the derived clones was conserved in subsequent progeny. Finally, it was established that inclusion of propidium iodide in the hybridoma cell sorting mixtures improved cloning efficiency by facilitating enhanced discrimination and elimination of nonviable cells. Our results indicate that flow cytometric-assisted single cell deposition provides positive attributes of several traditional hybridoma cloning techniques and, in addition, furnishes a tool for steering the cloning process toward selection of enhanced immunoglobulin producing cultures.
Marder
P
P
Lilly Research Laboratories, Indianapolis, Indiana 46285.
Maciak
R S
RS
Fouts
R L
RL
Baker
R S
RS
Starling
J J
JJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D002469:Cell Separation / Q000379:methods*; D002999:Clone Cells; D005434:Flow Cytometry / Q000379:methods*; D006825:Hybridomas / Q000276:immunology*; D007834:Lasers; D051379:Mice; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D011419:Propidium; D011947:Receptors, Antigen, B-Cell / Q000032:analysis
D000911:Antibodies, Monoclonal; D011947:Receptors, Antigen, B-Cell; D011419:Propidium