Effects of phospholipid on the structure of human apolipoprotein A-IV.
We have used fluorescence and circular dichroism spectroscopy to investigate the effect of phospholipid on the structure and molecular stability of human apolipoprotein A-IV (apo-A-IV). Binding of apo-A-IV to egg phosphatidylcholine vesicles was rapid and did not cause release of encapsulated 6-carboxyfluorescein. Fluorometric titration established that apo-A-IV bound to the vesicles with an association constant of 1.36 x 10(6) liters/mol and a binding maximum of 2 molecules per vesicle. Binding of apo-A-IV to the vesicle surface caused a progressive increase in alpha helicity from 43% at baseline to 83% at saturation; denaturation studies showed that the free energy of stabilization of binding was 6.31 kcal/mol. Fluorescence quenching studies revealed that binding of apo-A-IV to the vesicles was associated with a dramatic decrease in the fractional exposure of tyrosine to iodide, and a decrease in the efficiency of intramolecular tyrosine-tryptophan energy transfer. These findings suggest that the binding of apo-A-IV to the vesicle surface may involve a relaxation of the globular protein conformation in which the tyrosine containing alpha-helical domains surrounding the tryptophan "unfold" and reorient their hydrophobic faces toward the phospholipid monolayer, with a consequent induction of additional alpha-helical structure. However, our data also suggest that apo-A-IV does not penetrate deeply into the region of the phospholipid fatty acyl chains, but rather sits higher in the monolayer, intercalated between the charged phospholipid head groups. This characteristic may determine the labile interaction of apo-A-IV with high density lipoproteins.
Weinberg
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RB
Department of Medicine, University of Texas Health Science Center, Houston 77225.
Jordan
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MK
D001054:Apolipoproteins A / Q000378:metabolism; D002942:Circular Dichroism; D004735:Energy Transfer; D005452:Fluoresceins / Q000378:metabolism; D006801:Humans; D008081:Liposomes / Q000378:metabolism; D015394:Molecular Structure; D010713:Phosphatidylcholines / Q000378:metabolism; D010743:Phospholipids / Q000494:pharmacology*; D011487:Protein Conformation; D011489:Protein Denaturation; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics; D014364:Tryptophan; D014443:Tyrosine
D001054:Apolipoproteins A; D005452:Fluoresceins; D008081:Liposomes; D010713:Phosphatidylcholines; D010743:Phospholipids; C022205:apolipoprotein A-IV; C024098:6-carboxyfluorescein; D014443:Tyrosine; D014364:Tryptophan