Studies on the metabolism of gomisin A (TJN-101). II. Structure determination of biliary and urinary metabolites in rat.
After oral administration of gomisin A (1) to rats, the bile and urine were collected and treated with beta-glucuronidase and arylsulfatase. Seven metabolites, met B (2), met A-III (3), met E (4), met D (5), met F (6), met G (7), and met H (8) were isolated from the bile treated with the enzymes. Eight metabolites 2-8, and met A-II (9) were isolated from the urine treated with the enzymes. A major metabolite 2, and two minor metabolites 3 and 9 were identified as met B, met A-III, and met A-II, respectively, which are oxidative products of 1 formed by rat liver S9 mix. The structures of five new metabolites 4-7, and 8 were determined on the basis of chemical and spectral studies.
Ikeya
Y
Y
Research Institute for Biology & Chemistry, Tsumura & Co., Ibaraki, Japan.
Mitsuhashi
H
H
Sasaki
H
H
Matsuzaki
Y
Y
Matsuzaki
T
T
Hosoya
E
E
D000818:Animals; D001646:Bile / Q000378:metabolism*; D034242:Cyclooctanes; D004149:Dioxoles; D017705:Lignans; D008297:Male; D011083:Polycyclic Compounds / Q000378:metabolism* / Q000652:urine; D051381:Rats; D011919:Rats, Inbred Strains
D034242:Cyclooctanes; D004149:Dioxoles; D017705:Lignans; D011083:Polycyclic Compounds; C033585:schizandrol B