Platelet activating factor and one-kidney, one clip hypertension.
The reduction in blood pressure to normotensive levels within 3 hours of unclipping the one-kidney, one clip Goldblatt hypertensive rat has been attributed to the release of potent blood pressure-lowering lipids, one of which is thought to be identical to platelet activating factor. The specific platelet activating factor receptor antagonist WEB 2086 was infused intravenously into hypertensive one-kidney, one clip rats, and the mean arterial blood pressure changes after unclipping were examined. Before infusion, blocking doses of WEB 2086 were confirmed to effectively abolish the fall in blood pressure induced by exogenous platelet activating factor. Serotonin release in response to exogenous platelet activating factor was also inhibited in platelets preincubated with plasma from rats infused with the antagonist. Hypertensive rats were given a bolus blocking dose of WEB 2086 (5 mg/kg i.v.) and the same dose by infusion (5 mg/kg/hr i.v.) before they were unclipped. A control group was given a bolus volume of saline and infused with saline before unclipping. In WEB 2086-treated rats, blood pressure fell from a baseline mean of 181 +/- 13.0 to 125 +/- 23 mm Hg after 4 hours, a fall of 28%. Saline-treated rats fell from a mean of 194 +/- 23 to 127 +/- 25 mm Hg (33%). There was no significant difference in the blood pressure fall between the two groups. Therefore, platelet activating factor is unlikely to be responsible for the restoration of normal blood pressure after unclipping the Goldblatt hypertensive rat. We attribute the fall in blood pressure to other presently unidentified renomedullary lipids.
Cotter
J L
JL
Department of Medicine, University of Western Australia, Royal Perth Hospital, Perth.
Vandongen
R
R
Burton
D L
DL
Sturm
M J
MJ
D000818:Animals; D001381:Azepines / Q000494:pharmacology; D001792:Blood Platelets / Q000378:metabolism; D001794:Blood Pressure / Q000187:drug effects; D006977:Hypertension, Renal / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D010972:Platelet Activating Factor / Q000031:analogs & derivatives / Q000032:analysis / Q000037:antagonists & inhibitors / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D012077:Renal Artery / Q000601:surgery; D012701:Serotonin / Q000378:metabolism; D012964:Sodium / Q000652:urine; D014230:Triazoles / Q000494:pharmacology
D001381:Azepines; C029271:O-deacetyl platelet activating factor; D010972:Platelet Activating Factor; D014230:Triazoles; C052518:WEB 2086; D012701:Serotonin; D012964:Sodium