A simple, sensitive liquid chromatographic method for quantitation of D-galactose and D-glucose in blood plasma.
The concentrations of D-glucose and D-galactose in plasma of galactosemic rats were quantitatively measured by a liquid chromatographic method, based on retention of the weakly ionized monosaccharides by an anion exchange column under alkaline conditions, elution with 9 mmol/L NaOH, and electrochemical detection. This method is both simple and sensitive, since very dilute plasma samples can be directly analysed.
Griffin
B W
BW
Alcon Laboratories, Inc., Fort Worth, TX 76134.
Williams
G W
GW
D000818:Animals; D001786:Blood Glucose / Q000032:analysis*; D002853:Chromatography, Liquid; D004032:Diet; D005690:Galactose / Q000008:administration & dosage / Q000032:analysis; D005693:Galactosemias / Q000097:blood*; D008297:Male; D011786:Quality Control; D051381:Rats; D011919:Rats, Inbred Strains
D001786:Blood Glucose; D005690:Galactose