[The use of filamentous phage M13 in protein engineering].
M13B1 vector based on the filamentous phage M13 has been constructed. M13B1 phage carries the gene of resistance to ampicillin and contains the unique site of recognition for BamHI restriction endonuclease in gene VIII coding for the major coat protein. BamHI restriction site has been inserted into the gene of the major coat protein by means of oligonucleotide directed mutagenesis. The synthetic DNA fragment coding for the model peptides has been inserted through BamHI site into the M13B1 DNA. The possibility of inserting foreign peptides into the N-terminus at maintaining the viability of hybrid phages has been shown. The differences in specificity of the recombinant phage maturation have been determined by analysing the amino acid sequence of B-protein.
Il'ichev
A A
AA
Minenkova
O O
OO
Tat'kov
S I
SI
Karpyshev
N N
NN
Eroshkin
A M
AM
Ofitserov
V I
VI
Akimenko
Z A
ZA
Petrenko
V A
VA
Sandakhchiev
L S
LS
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D003090:Coliphages / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D015202:Protein Engineering; D011995:Recombination, Genetic
D036022:Capsid Proteins; D004279:DNA, Viral; D008565:Membrane Proteins; C025668:coat protein, Bacteriophage M13
Ispol'zovanie nitchatogo faga M13 dlia belkovoĭ inzhenerii.