Multiparametric analysis of peripheral blood in the normal pediatric population by flow cytometry.
Lymphocyte subset analysis was performed on 114 healthy children and 84 healthy adults. Samples were prepared by a whole blood lysis technique and analyzed by flow cytometry. The percentage and total number of CD3+, CD4+, CD8+, and CD19+ lymphocytes were calculated for each of six age groups. A direct correlation with age was seen in the percentages of CD3+, CD4+, and CD8+ lymphocytes. The absolute number and percentage of total lymphocytes, the percentage and absolute number of CD19+ lymphocytes, and the absolute number of CD3+ lymphocytes decreased with age. No significant correlation with age was observed for white blood cells, the absolute number of CD4+ and CD8+ lymphocytes, and the CD4+/CD8+ ratio.
Wiener
D
D
Department of Pathology, University of South Florida College of Medicine, Tampa.
Shah
S
S
Malone
J
J
Lowell
N
N
Lowitt
S
S
Rowlands
D T
DT
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D015703:Antigens, CD / Q000032:analysis*; D015415:Biomarkers; D002648:Child; D002675:Child, Preschool; D005434:Flow Cytometry; D006801:Humans; D007958:Leukocyte Count; D008214:Lymphocytes / Q000032:analysis* / Q000276:immunology; D008875:Middle Aged; D012016:Reference Values
D015703:Antigens, CD; D015415:Biomarkers