Novel pathways of oleic and cis-vaccenic acid biosynthesis by an enzymatic double-bond shifting reaction in higher plants.
The novel pathways of oleic acid formation from cis-vaccenic (cis-11-octadecenoic) acid and of cis-vaccenic acid formation from oleic acid by enzymatic positional isomerization have been proposed in higher plants, based on stable-isotope experiments using [2,2-2H2]cis-vaccenate or [2,2-2H2]oleate as an immediate precursor. A pulp homogenate and also pulp slices prepared from developing kaki (Diospyros kaki) fruit could catalyze these hitherto unknown isomerizations. This suggests the presence of a new type of isomerase responsible for the double-bond shifting reaction without cis-trans isomerization in the middle of fatty acid carbon chains.
Shibahara
A
A
Department of Nutrition, Kobe-Gakuin University, Japan.
Yamamoto
K
K
Takeoka
M
M
Kinoshita
A
A
Kajimoto
G
G
Nakayama
T
T
Noda
M
M
D055598:Chemical Phenomena; D002621:Chemistry; D007535:Isomerases / Q000378:metabolism; D007536:Isomerism; D019301:Oleic Acid; D009829:Oleic Acids / Q000096:biosynthesis*; D010944:Plants / Q000201:enzymology / Q000378:metabolism*
D009829:Oleic Acids; D019301:Oleic Acid; C065593:cis-vaccenic acid; D007535:Isomerases