Cobalt stimulates carotid body chemoreceptors.
Because cobalt administration is known to elicit erythropoietin response, it is a reasonable hypothesis that cobalt would also stimulate the O2-sensing process in the peripheral chemoreceptors. We tested this hypothesis by measuring the effects of cobalt chloride on carotid chemosensory fibers in pentobarbital-anesthetized cats that were paralyzed and artificially ventilated. Responses of carotid chemoreceptor afferents to graded doses of cobalt given by intra-arterial injections (0.08-2.10 mumols) were measured at constant blood gases. Responses of the same chemoreceptor afferents to hypoxia, before and after a saturation dose of cobalt, were measured. In two experiments carotid body tissue PO2 was also simultaneously measured. The chemosensory fibers showed prolonged excitation after a brief period of inhibition subsequent to cobalt administration. The stimulatory effect showed a dose-dependent saturation response. Cobalt augmented rather than blocked carotid chemoreceptor response to hypoxia. The effect of cobalt was not mediated by tissue PO2. These results are consistent with the hypothesis that cobalt stimulates the O2-sensing mechanism, although a direct effect of cobalt on the excitability of the chemosensory terminal remains a possibility.
Di Giulio
C
C
Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia.
Huang
W X
WX
Lahiri
S
S
Mokashi
A
A
Buerk
D G
DG
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002344:Carotid Body / Q000187:drug effects* / Q000502:physiology; D002415:Cats; D002628:Chemoreceptor Cells / Q000187:drug effects* / Q000502:physiology; D003035:Cobalt / Q000494:pharmacology*; D004594:Electrophysiology; D004921:Erythropoietin / Q000096:biosynthesis; D000860:Hypoxia / Q000503:physiopathology; D010100:Oxygen / Q000378:metabolism
D004921:Erythropoietin; D003035:Cobalt; D010100:Oxygen; D002118:Calcium