title: Production of carrier-free 66Ga and labeling of antimyosin antibody for positron imaging of acute myocardial infarction.
abstract: A method for labeling monoclonal antimyosin antibodies with 66Ga is described. The radionuclide is a positron emitter (t1/2: 9.4h) produced by means of the 63Cu (4He, n) 66Ga reaction. Purification of the 66Ga from the copper target is described in detail. Monoclonal antimyosin antibodies are labeled with 66Ga at a high yield (99%) by transcomplexation from an acetate buffer with the diethylenetriamine pentaacetic acid (DTPA)-labeled antibody and preliminary evaluated in two dogs for imaging of myocardial necrosis by positron emission tomography.
authors.affiliation: Laboratory of Analytical Chemistry, Faculty of Pharmaceutical Sciences, University Hospital, Gent, Belgium.
authors.forename: P
authors.initials: P
authors.lastname: Goethals
authors.forename: M
authors.initials: M
authors.lastname: Coene
authors.forename: G
authors.initials: G
authors.lastname: Slegers
authors.forename: D
authors.initials: D
authors.lastname: Vogelaers
authors.forename: J
authors.initials: J
authors.lastname: Everaert
authors.forename: I
authors.initials: I
authors.lastname: Lemahieu
authors.forename: F
authors.initials: F
authors.lastname: Colardyn
authors.forename: G R
authors.initials: GR
authors.lastname: Heyndrickx
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D004285:Dogs; D005710:Gallium Radioisotopes; D007553:Isotope Labeling; D009203:Myocardial Infarction / Q000000981:diagnostic imaging*; D009218:Myosins / Q000276:immunology*; D004369:Pentetic Acid; D011876:Radionuclide Generators; D014055:Tomography, Emission-Computed
chemical_list: D000911:Antibodies, Monoclonal; D005710:Gallium Radioisotopes; D004369:Pentetic Acid; D009218:Myosins