Effect of oxygenation level of virus-infected cell monolayer culture on the rate of viral multiplication.
The dependence of the virus multiplication rate on the oxygenation level of a virus-infected cell culture was quantitatively evaluated by the influence of agar overlay thickness on the viral plaque diameter. Although the experimental curve was found to fit the exponential function it reflects an interaction of at least two processes: gas diffusion through the agar medium and active 'pumping' of respiring cells determining the oxygen influx. Therefore, it has been deduced that the dependence of the virus multiplication rate on the oxygenation level fits the linear function, which is not the case for the most metabolic (enzymatic) reactions. The concept of intracellular unbalanced molecular constellations is suggested as an explanation of the linear dependence.
Lipkind
M
M
Division of Avian Diseases, Kimron Veterinary Institute, Beit Dagan, Israel.
D000818:Animals; D002478:Cells, Cultured; D009522:Newcastle disease virus / Q000254:growth & development*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D010948:Viral Plaque Assay
D010100:Oxygen