[Electrostatic method of determination of phospholipase A activity].
An electrostatic assay of phospholipase A activity has been developed. This assay is based on the monitoring of changes in the boundary potential of the planar lipid bilayer. The initial rate of the potential changes induced by phospholipase hydrolysis is proportional to the enzyme activity. The sensitivity of the method is up to 0.0002 Units/ml; the effect of impurities is ignorable. The method allows the use of natural phospholipase substrates and is rapid, i.e., the phospholipase activity assay takes approximately 5 minutes.
Chernyĭ
V V
VV
Mirskiĭ
V M
VM
Sokolov
V S
VS
Markin
V S
VS
D004560:Electricity; D008051:Lipid Bilayers / Q000032:analysis; D008564:Membrane Potentials; D008722:Methods; D010740:Phospholipases / Q000032:analysis*; D010741:Phospholipases A / Q000032:analysis*
D008051:Lipid Bilayers; D010740:Phospholipases; D010741:Phospholipases A
Elektrostaticheskiĭ metod opredeleniia aktivnosti fosfolipazy A.