Characterization of cDNAs of the human pregnancy-specific beta 1-glycoprotein family, a new subfamily of the immunoglobulin gene superfamily.
Three highly homologous cDNAs encoding human pregnancy-specific beta 1-glycoprotein (SP1) were isolated from a human placental cDNA library. These cDNAs share greater than 90% nucleotide homology in their coding sequences, and greater than 79% of the encoded amino acids are homologous. Proteins encoded by these cDNAs are very similar to members of the carcinoembryonic antigen family and contain repeating domains, conserved disulfide bridges, and beta-sheet structure typical of the immunoglobulin gene superfamily. However, the high degree of sequence homology and relatively lesser degree of glycosylation among the SP1 proteins suggest that they exist as a unique family instead of being members of the CEA family. Both soluble and potentially membrane-bound forms of SP1 proteins were present in the placenta. Northern blot analysis using specific probes confirmed the expression of multiple mRNA species in human term placenta.
Zheng
Q X
QX
Department of Pediatrics, Georgetown University Medical Center, Washington, D.C. 20007.
Tease
L A
LA
Shupert
W L
WL
Chan
W Y
WY
D000595:Amino Acid Sequence; D001483:Base Sequence; D002272:Carcinoembryonic Antigen / Q000235:genetics; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D003429:Cross Reactions; D004247:DNA / Q000032:analysis*; D005803:Genes, Immunoglobulin; D006801:Humans; D008969:Molecular Sequence Data; D010920:Placenta / Q000032:analysis*; D011257:Pregnancy Proteins / Q000235:genetics*; D011268:Pregnancy-Specific beta 1-Glycoproteins / Q000235:genetics*; D012333:RNA, Messenger
D002272:Carcinoembryonic Antigen; D011257:Pregnancy Proteins; D011268:Pregnancy-Specific beta 1-Glycoproteins; D012333:RNA, Messenger; D004247:DNA