title: Reconstitution of rapeseed high molecular weight protein from isolated subunits.
abstract: The high molecular weight protein was isolated from rapeseed and characterised. Six subunits were isolated in SDS (0.01%) solution on polyacrylamide-gel electrophoresis and by gel filtration on Sephadex G-100. Reassociation by removing SDS by co-dialysis, against 10 mM sodium phosphate buffer (pH 7.9) was done and the yield was about 90%. The reconstituted protein was indistinguishable from the intact protein in all respects. The subunits isolated from the native protein and the reconstituted protein were found to have identical molecular weights and N-terminal amino acids. No disulphide bonds were observed in the subunit association. Amino acid analysis of the proteins and the six subunits was performed and the number of each amino acid residue calculated.
authors.affiliation: Department of Chemistry, University of Roorkee, India.
authors.forename: R
authors.initials: R
authors.lastname: Bhushan
authors.forename: V K
authors.initials: VK
authors.lastname: Mahesh
authors.forename: P V
authors.initials: PV
authors.lastname: Mallikharjun
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D001937:Brassica; D046911:Macromolecular Substances; D010940:Plant Proteins / Q000032:analysis / Q000302:isolation & purification*
chemical_list: D000596:Amino Acids; D046911:Macromolecular Substances; D010940:Plant Proteins