Decreased mitogenic activity in hypercholesterolaemic plasma.
The mitogenic activity on rat aortic smooth muscle cells of plasma from patients with hypercholesterolaemia was compared to that of plasma from a normocholesterolaemic control group and to that obtained after lowering plasma cholesterol levels in the hypercholesterolaemic groups with simvastatin. Mean plasma total and low density lipoprotein cholesterol and apolipoprotein B concentrations in the two groups were 8.62 +/- 0.62 mmol l-1, 7.03 +/- 0.56 mmol l-1 and 1.96 +/- 0.14 g l-1, respectively, and 5.05 +/- 0.20 mmol l-1, 3.42 +/- 0.18 mmol l-1 and 0.80 +/- 0.04 g l-1, respectively, all differences significant at P less than 0.001. During simvastatin treatment these parameters decreased to 5.70 +/- 0.35, 4.15 +/- 0.33 mmol l-1 and 1.33 +/- 0.08 g l-1, all changes being significantly (P less than 0.001) lower than before treatment. Hyperlipidaemic plasma in untreated patients had a significantly lower mitogenic activity than normolipidaemic control plasma as measured by autoradiographic labelling of DNA after 3H-thymidine incorporation, 25.7 +/- 1.8% vs. 32.0 +/- 1.9%, P less than 0.05. Lowering plasma cholesterol levels in the hyperlipidaemic patients 'normalized' the mitogenic activity to 32.3 +/- 1.6%, P less than 0.05. The reason for this unexpected finding is not known.
Mölgaard
J
J
Department of Internal Medicine, Faculty of Health Sciences, University Hospital, Linköping, Sweden.
Carlsson
M
M
Nilsson
J
J
Olsson
A G
AG
D000328:Adult; D000368:Aged; D000818:Animals; D000924:Anticholesteremic Agents / Q000494:pharmacology; D001055:Apolipoproteins B / Q000097:blood; D002478:Cells, Cultured; D002784:Cholesterol / Q000097:blood; D004247:DNA / Q000096:biosynthesis; D005260:Female; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood*; D008074:Lipoproteins / Q000097:blood; D008148:Lovastatin / Q000031:analogs & derivatives / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D008934:Mitogens / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D051381:Rats; D019821:Simvastatin
D000924:Anticholesteremic Agents; D001055:Apolipoproteins B; D008074:Lipoproteins; D008934:Mitogens; D004247:DNA; D002784:Cholesterol; D008148:Lovastatin; D019821:Simvastatin