Steroid binding alterations in tissue compartments of the vagina of control and neonatally diethylstilbestrol-treated adult mice.
Steroid binding in both the vaginal epithelium and the vaginal fibromuscular wall (FMW) was compared in control and neonatally estrogen-treated mice. Neonatal treatment with a low dose of the estrogen diethylstilbestrol (DES) had no significant effect on adult estrogen binding within the assayed vaginal compartments; however, this treatment caused a 2-fold increase in the level of cytosolic progestin binding in the vaginal FMW over that in vehicle-treated mice. This low neonatal dose did not affect the level of progestin binding in the vaginal epithelium. In contrast, neonatal treatment with a larger dose of DES caused marked increases in cytosolic progestin binding, decreases in cytosolic estrogen binding, and increases in nuclear estrogen binding within the FMW. Furthermore, as a result of the changes in specific binding induced by the neonatal DES treatment, the degree of the estrogen binding within in each tissue shifted from a predominantly cytosolic site to a nuclear one.
Eiger
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S
Department of Integrative Biology, University of California, Berkeley 94720.
Mills
K T
KT
Bern
H A
HA
D000818:Animals; D000831:Animals, Newborn; D002451:Cell Compartmentation; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004054:Diethylstilbestrol / Q000494:pharmacology*; D004967:Estrogens / Q000378:metabolism*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D011372:Progestins / Q000378:metabolism; D011960:Receptors, Estrogen / Q000187:drug effects / Q000378:metabolism*; D014621:Vagina / Q000187:drug effects / Q000378:metabolism*
D004967:Estrogens; D011372:Progestins; D011960:Receptors, Estrogen; D004054:Diethylstilbestrol