[Electroelution of protein fractions from a polyacrylamide gel].
A method for electroelution of protein fractions from polyacrylamide gel and device for performing such a process have been developed. The application of two tris-glycine buffers with the low and high ionic strength, pH 9.0-9.2 provides a concentration of protein simultaneously to extraction from the gel. The duration of elution is in the range of 1-3 hours and depends on the protein mobility. The effectiveness of the system is demonstrated for disc-electrophoretic separation and electrophoresis in slab gel in the presence of SDS. The maximal amount of pure protein fraction obtained is about 4.5-5.0 mg. The method may be useful especially for the fractionation of limited quantities of protein samples.
Lutsyk
M D
MD
Oleshko
P S
PS
Vovkanych
A S
AS
D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods*; D011506:Proteins / Q000302:isolation & purification*
D011506:Proteins
Elektroeliuiuvannia bilkovykh fraktsiĭ z poliakrylamidnogo geliu.