Determination of erythromycin base and 2'-acetylerythromycin in human plasma using high-performance liquid chromatography with electrochemical detection.
A high-performance liquid chromatographic method for the determination of plasma concentrations of erythromycin base and 2'-acetylerythromycin, an ester prodrug of erythromycin, is described. tert.-Butyl methyl ether extracts of 1-ml plasma samples (pH 10) were chromatographed on a C18 reversed-phase column. A three-electrode coulometric detector (oxidation potentials +0.65 and +0.85 V) was used for quantitation. Oleandomycin was used as an internal standard. The method has good precision and accuracy, is linear in the range 0.25-7.5 mg/l and has proved to be suitable for pharmacokinetic studies in humans. Correlation with a microbiological assay was satisfactory (r greater than or equal to 0.95), but the chromatographic method gave ca. 30% higher values.
Laakso
S
S
Research Centre, Farmos Group Ltd., Turku, Finland.
Scheinin
M
M
Anttila
M
M
D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D004566:Electrodes; D004917:Erythromycin / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009827:Oleandomycin / Q000097:blood; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D013696:Temperature
D004917:Erythromycin; C056625:erythromycin 2'-acetate; D009827:Oleandomycin