Dissociation between allogeneic T cell stimulation and interleukin-1 or tumor necrosis factor production by human lung dendritic cells.
A small portion of human lung mononuclear cells are very potent stimulators of allogeneic resting T cells. Although several-fold more effective than phagocytic alveolar macrophages (AM) and blood monocytes (Mo), they do not produce more of the lymphocyte co-stimulators interleukin-1-alpha (IL-1 alpha), interleukin-1-beta (IL-1 beta), or tumor necrosis factor-alpha (TNF-alpha) than did Mo. Blocking antibodies against IL-1 alpha, IL-1 beta, TNF-alpha, and IL-6 did not reduce T cell proliferation. These potent antigen-presenting cells (APC) are loosely adherent and do not have phagocytic inclusions. Most of them have the marker RFD1 of dendritic cells (DC) rarely present on Mo or AM and have a strong tendency to form clusters with T cells like murine DC. Thus, we demonstrate an example in the human system of a dissociation between T cell activation and IL-1 or TNF-alpha production by DC or Mo, implying a major role for other "co-stimulating signals" by lung APC with dendritic features. The presence of different APC with various co-stimulating signals may be of importance for T cell subsets modulation.
Nicod
L P
LP
Pulmonary Division, University Hospital, Geneva, Switzerland.
Galve-de Rochemonteix
B
B
Dayer
J M
JM
D000906:Antibodies / Q000276:immunology; D000938:Antigen-Presenting Cells / Q000187:drug effects / Q000276:immunology; D001667:Binding, Competitive; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008168:Lung / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology*; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D000906:Antibodies; D007375:Interleukin-1; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha