[NAD-dependent formate dehydrogenase from methylotrophic bacteria Pseudomonas sp. 101. I. Amino acid sequence].
The primary structure of NAD-dependent formate dehydrogenase from methylotrophic bacterium Pseudomonas sp. 101 is determined. The enzyme is composed of two identical subunits, each comprising 393 amino acid residues, and has a molecular weight of 43.1 kD. To elucidate the protein's amino acid sequence, four types of digestion were used: cyanogen bromide cleavage at methionine residues, endoproteinase Lys-C digestion at lysine residues, endoproteinase Glu-C cleavage at glutamic acid residues, and tryptic digestion. The peptides obtained were purified to homogeneity and characterized.
Popov
V O
VO
Shumilin
I A
IA
Ustinnikova
T B
TB
Lamzin
V S
VS
Egorov
Ts A
TsA
D000445:Aldehyde Oxidoreductases / Q000032:analysis*; D000595:Amino Acid Sequence; D005560:Formate Dehydrogenases / Q000032:analysis* / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011549:Pseudomonas / Q000201:enzymology*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D005560:Formate Dehydrogenases; D000445:Aldehyde Oxidoreductases
NAD-zavisimaia formiatdegidrogenaza metilotrofnykh bakteriĭ Pseudomonas sp. 101. I. Aminokislotnaia posledovatel'nost'.