Radial "high-performance" thin-layer chromatography used to assess fetal lung maturity.
A radial "high-performance" thin-layer chromatographic (HPTLC) method is described by which the percentages and ratios of phosphatidylinositol, sphingomyelin, lecithin, phosphatidylethanolamine, phosphatidylglycerol, and dimethyl phosphatidylethanolamine may be determined simultaneously. An additional method for radial HPTLC determination of saturated phosphatidylcholine is described. We report results of application of these methods to greater than 2000 specimens of amniotic fluid from both diabetic and nondiabetic cases.
Mackenzie
J R
JR
Clinical Chemistry Laboratory, Methodist Hospital of Indiana, Indianapolis 46202.
Truesdale
M
M
D000649:Amniocentesis; D000653:Amniotic Fluid / Q000032:analysis / Q000166:cytology; D002855:Chromatography, Thin Layer; D003720:Densitometry; D005260:Female; D005325:Fetal Organ Maturity; D006801:Humans; D008055:Lipids / Q000032:analysis; D008168:Lung / Q000166:cytology / Q000196:embryology*; D010713:Phosphatidylcholines / Q000302:isolation & purification; D010743:Phospholipids / Q000302:isolation & purification; D011247:Pregnancy; D011254:Pregnancy in Diabetics / Q000378:metabolism
D008055:Lipids; D010713:Phosphatidylcholines; D010743:Phospholipids