title: PCR-based site-directed mutagenesis using primers with mismatched 3'-ends.
authors.affiliation: Zentrum für Molekulare Biologie, Universität Heidelberg, FRG.
authors.forename: M
authors.initials: M
authors.lastname: Nassal
authors.forename: A
authors.initials: A
authors.lastname: Rieger
mesh_terms: D001483:Base Sequence; D008969:Molecular Sequence Data; D009154:Mutation; D021141:Nucleic Acid Amplification Techniques; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction / Q000379:methods*; D013698:Templates, Genetic
chemical_list: D009838:Oligodeoxyribonucleotides