title: Lysozyme induced fusion of negatively charged phospholipid vesicles.
abstract: Lysozyme promotes fusion of negatively charged phospholipid vesicles prepared by ethanolic injection. Vesicle fusion was a leaky process as revealed by the release of encapsulated carboxyfluorescein or Tb-DPA complex. Extensive proteolysis of lysozyme inhibited the fusion process. The fusion process was critically dependent on the medium ionic strength; 100 mM of any salt was sufficient to inhibit totally the fusion activity of the protein. The high efficiency of lysozyme (80% RET) was almost constant in the pH range from 4.0 to 9.0, but it was sharply diminished when the pH of the medium was at the isoelectric point of the protein (pI 11.0). Fusion induced by chemically modified lysozyme, showed that the pH profile changed according to the isoelectric point of the protein derivative. These observations stress the importance of electrostatic interactions in the process of fusion induced by lysozyme.
authors.affiliation: Departamento de Bioquimica de la Nutrición, Instituto de Química Biológica Dr. Bernabé Bloj CONICET-UNT, Tucumàn, Argentina.
authors.forename: E
authors.initials: E
authors.lastname: Posse
authors.forename: A
authors.initials: A
authors.lastname: López Viñals
authors.forename: B F
authors.initials: BF
authors.lastname: de Arcuri
authors.forename: R N
authors.initials: RN
authors.lastname: Farías
authors.forename: R D
authors.initials: RD
authors.lastname: Morero
mesh_terms: D002462:Cell Membrane / Q000648:ultrastructure; D007700:Kinetics; D008051:Lipid Bilayers / Q000378:metabolism; D008561:Membrane Fusion / Q000187:drug effects*; D009113:Muramidase / Q000494:pharmacology*; D010743:Phospholipids / Q000378:metabolism*
chemical_list: D008051:Lipid Bilayers; D010743:Phospholipids; D009113:Muramidase