title: High-performance liquid chromatographic determination of zinc pyrithione in antidandruff preparations based on copper chelate formation.
abstract: A simple and rapid method for the determination of zinc pyrithione (ZPT) in antidandruff preparations by high-performance liquid chromatography (HPLC) has been developed. ZPT in samples was converted into a stable copper(II) complex by mixing with cupric sulphate solution followed by extraction with chloroform. HPLC was carried out on a Nucleosil 5 C18 column (15 cm X 4.6 mm I.D.) using methanol-water (3:2) as the mobile phase with UV detection at 320 nm. The calibration graph was linear from 0.1-0.5 micrograms for ZPT. The recoveries from four shampoos were 98.0-100.6% with high accuracy.
authors.affiliation: Public Health Laboratory of Chiba Prefecture, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nakajima
authors.forename: T
authors.initials: T
authors.lastname: Yasuda
authors.forename: H
authors.initials: H
authors.lastname: Nakazawa
mesh_terms: D002614:Chelating Agents; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D003300:Copper; D006203:Hair Preparations / Q000032:analysis*; D006801:Humans; D009942:Organometallic Compounds; D011725:Pyridines / Q000032:analysis*; D015032:Zinc / Q000032:analysis*
chemical_list: D002614:Chelating Agents; D006203:Hair Preparations; D009942:Organometallic Compounds; D011725:Pyridines; D003300:Copper; D015032:Zinc; C010423:pyrithione zinc