Rapid and simple method for the determination of nimustine hydrochloride in human blood and brain by high-performance liquid chromatography.
A simple method for the determination of nimustine hydrochloride in blood and brain by high-performance liquid chromatography was developed. A pH 4.52 buffer was used in the extraction from blood and a pH 5.0 buffer was used for brain. A pre-packed Extrelut column was used to make the extraction procedure uncomplicated. At room temperature light-resistant test-tubes were unnecessary. The lower limit of detection was 50 ng/ml for blood and 100 ng/g for brain. This method may be useful for the determination of nimustine hydrochloride in blood and brain samples from patients.
Tatsuhara
T
T
Department of Hospital Pharmacy, Tottori University, School of Medicine, Japan.
Tabuchi
F
F
Yamane
M
M
Hori
T
T
D000818:Animals; D001322:Autoanalysis; D001923:Brain Chemistry; D002021:Buffers; D002851:Chromatography, High Pressure Liquid; D006207:Half-Life; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015376:Nimustine / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats
D002021:Buffers; D015376:Nimustine