Combined effect in vitro of chemotherapy with agents acting on the cell membrane of Lewis lung carcinoma.
The treatment of cancer is often impeded by the emergence of drug-resistant clones. Drug resistance is in many cases due to a decreased drug accumulation in the tumor cell. Membrane-acting agents, by increasing cell permeability, might counteract the loss of sensitivity to drugs. In the present study, the effect of two membrane-acting agents, hyperthermia and the calcium channel blocker verapamil, on the action of adriamycin (ADR) on Lewis lung carcinoma (3LL) was examined. Hyperthermia increased drastically the antitumoral effect of ADR. The effectiveness of the combined ADR-hyperthermia treatment was proportional to the ADR dose and to the degree of hyperthermia. Verapamil had a similar effect but at higher ADR doses. Treatment modalities designed for the circumvention of drug resistance could be one approach in the attempt to find a way to control cancer.
Siegal
A
A
Department of Pathology, Sackler Faculty of Medicine, Tel Aviv University, Israel.
Hoenig
S
S
Leibovici
J
J
D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D002277:Carcinoma / Q000188:drug therapy* / Q000628:therapy; D002462:Cell Membrane; D002463:Cell Membrane Permeability; D004317:Doxorubicin / Q000494:pharmacology*; D004351:Drug Resistance; D006801:Humans; D006979:Hyperthermia, Induced; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000628:therapy; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013696:Temperature; D014700:Verapamil / Q000494:pharmacology*
D004317:Doxorubicin; D014700:Verapamil