Haptoglobin typing of human bloodstains using a specific DNA probe.
The stability of DNA in human bloodstains and various post mortem tissues has been investigated. High molecular weight (HMW) DNA was usually recovered from dried bloodstains, even those up to a few years old, but very rapid degradation was found to occur post mortem in the liver, pancreas, spleen and kidney. Other tissues such as the heart, thyroid and skeletal muscle were found to give a reasonable yield of HMW DNA during the first few days after death. The feasibility of using DNA extracted from forensic bloodstain specimens for the detection of DNA polymorphisms was explored using a human haptoglobin (Hp) alpha chain specific probe. Using HindIII and XbaI digests the Hp genotypes Hp2, Hp1F and Hp1S were distinguished by Southern blot analysis in DNA prepared from 1 cm2 bloodstains up to 15-18 months old.
Yokoi
T
T
Department of Forensic Medicine, Tohoku University School of Medicine, Sendai, Japan.
Sagisaka
K
K
D001345:Autoradiography; D001793:Blood Preservation; D001801:Blood Stains; D015139:Blotting, Southern; D004247:DNA / Q000032:analysis*; D015342:DNA Probes; D004587:Electrophoresis, Agar Gel; D005838:Genotype; D006242:Haptoglobins / Q000032:analysis / Q000235:genetics*; D006801:Humans; D015183:Restriction Mapping
D015342:DNA Probes; D006242:Haptoglobins; D004247:DNA