title: Sympathetic reactivity of cerebral arteries in developing fetal lamb and adult sheep.
abstract: The response of cerebral arteries to norepinephrine was examined in vivo in six dated preterm fetal (94-121 days gestation), eight term fetal (127-141 days gestation), five newborn (7-14 days), and five adult sheep, anesthetized and equipped with a closed cranial window. Norepinephrine (10(-8)-10(-4) M in cerebrospinal fluid) caused a dose-dependent decrease in pial arteriolar diameter in fetal and newborn lambs; however, preterm fetuses were 7- and 14-fold more sensitive to norepinephrine than term fetuses and newborn lambs, respectively. The effective concentration of norepinephrine inducing a 15% decrease in diameter (EC15) was 4.6 +/- 1.8, 33 +/- 11, and 64 +/- 23 microM, for the respective ages. Adult cerebral arterioles did not contract to norepinephrine. In preterm and term fetuses, the contractile response to norepinephrine was blocked by alpha 1-antagonist, prazosin (3 mg iv), and was enhanced by cocaine (10(-5) M; EC15 = 0.086 +/- 0.04 and 1.84 +/- 1.20 microM, respectively) indicating that alpha 1-adrenoceptors mediate the response and that the decrease in sensitivity is not caused by development of neuronal uptake processes. In seven fetuses (111-141 days; mean 123 days gestation), electrical stimulation of the superior cervical sympathetic ganglion constricted pial arterioles by 21 +/- 5%; this contractile response was also blocked by prazosin. The cerebral arterioles of the fetus in utero possess a functional sympathetic innervation capable of influencing cerebrovascular resistance. There is a loss of responsiveness of cerebral arterioles to norepinephrine during fetal and postnatal development, suggesting that the contribution of neuroadrenergic mechanisms to cerebrovascular regulation may be relatively unique to the immature brain.
authors.affiliation: Department of Physiology, University of Pennsylvania School of Medicine, Philadelphia 19104.
authors.forename: L C
authors.initials: LC
authors.lastname: Wagerle
authors.forename: C D
authors.initials: CD
authors.lastname: Kurth
authors.forename: R A
authors.initials: RA
authors.lastname: Roth
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn; D001160:Arterioles / Q000502:physiology; D002536:Cerebral Arteries / Q000294:innervation*; D003042:Cocaine / Q000494:pharmacology; D004558:Electric Stimulation; D005333:Fetus / Q000502:physiology*; D009638:Norepinephrine / Q000494:pharmacology; D010841:Pia Mater / Q000098:blood supply; D011224:Prazosin / Q000494:pharmacology; D012756:Sheep / Q000196:embryology; D013564:Sympathetic Nervous System / Q000502:physiology*
chemical_list: D003042:Cocaine; D009638:Norepinephrine; D011224:Prazosin