An infrared heat source for temperature control of a small microscope flow bath.
A simple, inexpensive, and easily installed infrared heat source with temperature controller for small superfusion baths used in electrophysiologic experiments is described. The output of a thermal sensor in the bath is amplified and sent to a feedback circuit which in turn controls the voltage supplied to a tungsten/halogen bulb focused on the bath. Fluctuation from the set point is no more than 0.2 degrees C with good fluid level control. Judging from the lack of change in the cardiac action potential duration, heart cells do not preferentially absorb infrared radiation.
Hewett
K W
KW
Division of Pediatric Cardiology, Medical University of South Carolina, Charleston 29425.
Francis
W W
WW
D000200:Action Potentials / Q000502:physiology; D000818:Animals; D001494:Baths; D004285:Dogs; D004867:Equipment Design; D006321:Heart / Q000502:physiology; D006361:Heating / Q000295:instrumentation*; D066298:In Vitro Techniques; D007259:Infrared Rays; D008853:Microscopy / Q000379:methods*; D013696:Temperature