Antilens antibodies in cataract and inflammatory eye disease: an evaluation of a new technique.
A new technique measuring serum antoantibodies to lens proteins, employing antigen linked to magnetisable cellulose particles and fluorescent end-point detection, was used to examine patients with senile cataract and inflammatory eye disease. 40% of patients with senile cataract had antibodies to lens proteins, as did 28% of patients with heterochromic cyclitis and 20% of patients with uveitis, while there were no positive sera from patients with scleritis. The single patient with lens-induced uveitis had a high titre, but so did some patients with senile cataract. With the incorporation of a standard curve this technique offers a reliable quantitative assay for lens autoantibodies. It may provide a valuable tool for the investigation of pathogenetic mechanisms in cataract and inflammatory eye disease.
Patel
M
M
Department of Pathology, Institute of Ophthalmology, London, United Kingdom.
Shine
B
B
Murray
P I
PI
D001323:Autoantibodies / Q000032:analysis* / Q000276:immunology; D002386:Cataract / Q000276:immunology*; D002482:Cellulose; D003459:Crystallins / Q000276:immunology; D005069:Evaluation Studies as Topic; D005128:Eye Diseases / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D015863:Iridocyclitis / Q000276:immunology; D007908:Lens, Crystalline / Q000276:immunology*; D012016:Reference Values; D015423:Scleritis / Q000276:immunology; D014605:Uveitis / Q000276:immunology
D001323:Autoantibodies; D003459:Crystallins; D002482:Cellulose