Structure and regulation of rat long-chain acyl-CoA synthetase.
Complementary DNAs encoding rat long-chain acyl-CoA synthetase have been isolated. The cDNAs were identified using synthetic oligonucleotide probes based on partial amino acid sequences of lysyl endopeptidase peptides of the purified enzyme. Rat long-chain acyl-CoA synthetase is predicted to contain 699 amino acid residues and to have a calculated molecular weight of 78,177. Significant sequence similarity was found between parts of long-chain acyl-CoA synthetase and firefly luciferase. Based on the similarity of the reaction mechanisms of the two enzymes, we propose a function for the similar region. The long-chain acyl-CoA synthetase mRNA is expressed in liver, heart, and epididymal adipose tissues and, to a much lesser extent, in brain, small intestine, and lung. The level of long-chain acyl-CoA synthetase mRNA is increased 7-8-fold in rat liver by feeding a diet high in carbohydrate or fat, consistent with the physiological significance of the enzyme in fatty acid metabolism.
Suzuki
H
H
Tohoku University Gene Research Center, Sendai, Japan.
Kawarabayasi
Y
Y
Kondo
J
J
Abe
T
T
Nishikawa
K
K
Kimura
S
S
Hashimoto
T
T
Yamamoto
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003066:Coenzyme A Ligases / Q000235:genetics* / Q000302:isolation & purification; D001517:Coleoptera / Q000235:genetics; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005786:Gene Expression Regulation; D015723:Gene Library; D008156:Luciferases / Q000235:genetics; D008862:Microsomes, Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D009928:Organ Specificity; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D012097:Repressor Proteins; D015183:Restriction Mapping; D029701:Saccharomyces cerevisiae Proteins; D012689:Sequence Homology, Nucleic Acid; D000098859:Long-Chain-Fatty-Acid-CoA Ligase
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D004247:DNA; D008156:Luciferases; D003066:Coenzyme A Ligases; C090799:FAA2 protein, S cerevisiae; D000098859:Long-Chain-Fatty-Acid-CoA Ligase