Class I (H-2Kb) gene transfection reduces susceptibility of YAC-1 lymphoma targets to natural killer cells.
A "hybrid gene" (MTKb) comprised of the human metallothionein IIA promoter ligated to the genomic sequence of the major histocompatibility complex class I (H-2Kb) gene was subcloned into the expression vector pSV2neo and transfected into the natural killer (NK) cell-sensitive YAC-1 lymphoma. The Kb gene product was readily detectable on the cell surface of G418-resistant transfectants using both Kb-specific monoclonal antibodies and H-2b-specific cytolytic T cells. Unlike control pSV2neo transfectants, MTKb-pSV2neo transfectants were relatively resistant to lysis by NK cells from H-2a, H-2b, H-2k or H-2 (a x b)F1 haplotype mice. These data strongly suggest that the effects of MHC expression on susceptibility to NK cells can be mediated by a single and well-defined class I molecule, Kb.
Carlow
D A
DA
Mount Sinai Hospital Research Institute, University of Toronto, Ontario, Canada.
Payne
U
U
Hozumi
N
N
Roder
J C
JC
Czitrom
A A
AA
D000818:Animals; D002999:Clone Cells; D005260:Female; D006183:H-2 Antigens / Q000235:genetics*; D007694:Killer Cells, Natural / Q000276:immunology*; D008223:Lymphoma / Q000276:immunology*; D008668:Metallothionein / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014162:Transfection; D015032:Zinc / Q000494:pharmacology
D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D008668:Metallothionein; D015032:Zinc