title: Collagen shield delivery of amphotericin B.
abstract: By using a high-pressure liquid chromatography assay, we investigated the ability of collagen shield therapeutic contact lenses to release amphotericin B and deliver it to the anterior segment of rabbit eyes. In vitro studies showed that presoaked collagen shields released most of the amphotericin B within the first hour of elution. We compared the corneal and aqueous humor amphotericin B levels produced by collagen shields soaked in amphotericin B and frequent-drop therapy at four time points over a six-hour period. The collagen shields soaked in amphotericin B produced corneal levels that were higher than those produced by frequent-drop therapy at one hour, equivalent to drop therapy at two and three hours, and lower than drop therapy at six hours. There were no differences in amphotericin B levels in aqueous humor at any time point between rabbits treated with collagen shield delivery and rabbits treated with frequent-drop delivery. The results of this study suggest that amphotericin B delivery to the cornea by collagen shields is comparable to frequent-drop delivery but has the potential benefit of added convenience and compliance.
authors.affiliation: Jules Stein Eye Institute, UCLA Center for Health Sciences 90024-7000.
authors.forename: S D
authors.initials: SD
authors.lastname: Schwartz
authors.forename: S A
authors.initials: SA
authors.lastname: Harrison
authors.forename: R E
authors.initials: RE
authors.lastname: Engstrom
authors.forename: R E
authors.initials: RE
authors.lastname: Bawdon
authors.forename: D A
authors.initials: DA
authors.lastname: Lee
authors.forename: B J
authors.initials: BJ
authors.lastname: Mondino
mesh_terms: D000287:Administration, Topical; D000666:Amphotericin B / Q000008:administration & dosage* / Q000493:pharmacokinetics; D000818:Animals; D000867:Anterior Chamber / Q000378:metabolism; D001082:Aqueous Humor / Q000378:metabolism; D001458:Bandages; D001684:Biological Dressings; D003094:Collagen; D003261:Contact Lenses; D003315:Cornea / Q000378:metabolism; D005260:Female; D011817:Rabbits
chemical_list: D000666:Amphotericin B; D003094:Collagen