Leakage of thyroid hormone-inducible monoamine oxidase inhibitor from rat liver cytosol.
T4-induced endogenous monoamine oxidase (MAO) inhibitor in rat liver perfusate by perfusion with sucrose buffer and lipid peroxide (LPO) and superoxide dismutase (SOD) in rat liver tissues were investigated after pretreatment with total hepatic ischemia. When the gel filtration of these rats liver perfusate were carried out using Sephadex G-25, fraction 18-20 were found to inhibit MAO activity. LPO of these rats were increased, but SOD activity was decreased. The accumulation of lipid peroxides would partly result from decreased activity of SOD as a consequence of membrane disorders. These results suggest that T4-induced MAO inhibitor in rat cytosol was released into plasma by membrane disorder.
Obata
T
T
Department of Pharmacology, Medical College of Oita, Japan.
Yamanaka
Y
Y
D000818:Animals; D003600:Cytosol / Q000201:enzymology / Q000378:metabolism*; D007511:Ischemia; D008054:Lipid Peroxides / Q000378:metabolism; D008099:Liver / Q000098:blood supply / Q000201:enzymology* / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology; D008995:Monoamine Oxidase / Q000378:metabolism; D008996:Monoamine Oxidase Inhibitors / Q000097:blood / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity; D013482:Superoxide Dismutase / Q000378:metabolism; D013974:Thyroxine / Q000494:pharmacology*; D013997:Time Factors
D008054:Lipid Peroxides; D008996:Monoamine Oxidase Inhibitors; D013482:Superoxide Dismutase; D008995:Monoamine Oxidase; D013974:Thyroxine