title: Measurement of retinal adhesive force in the in vivo rabbit eye.
abstract: A new method to evaluate the retinal adhesive force, which was defined as the force needed to achieve adhesive failure per unit length, in the in vivo rabbit eye was tested. A small dome-shaped retinal detachment (bleb) was made in the posterior pole by injecting balanced salt solution into the subretinal space. A separate measuring micropipette with a tip diameter of 5 microns was inserted into the subretinal space. The subretinal pressure was measured directly with the resistance servonulling method as the solution was injected repeatedly into the subretinal space. According to Laplace's law, the retinal adhesive force per unit length in normal Dutch rabbits was calculated to be (1.8 +/- 0.2) X 10(2) dyn/cm (n = 87). This value was about five times larger than that reported previously with in vitro peeling methods. The new method will resolve several problems encountered in the previous methods and will allow for the measurement of the retinal adhesive force more physiologically and precisely.
authors.affiliation: Department of Ophthalmology, Faculty of Medicine, Kyoto University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kita
authors.forename: A
authors.initials: A
authors.lastname: Negi
authors.forename: S
authors.initials: S
authors.lastname: Kawano
authors.forename: Y
authors.initials: Y
authors.lastname: Honda
authors.forename: S
authors.initials: S
authors.lastname: Maegawa
mesh_terms: D000268:Adhesiveness; D000818:Animals; D007267:Injections; D008433:Mathematics; D009885:Ophthalmology / Q000379:methods*; D011312:Pressure; D011817:Rabbits; D012160:Retina / Q000502:physiology*; D012965:Sodium Chloride
chemical_list: D012965:Sodium Chloride