title: Characterization and description of "Campylobacter upsaliensis" isolated from human feces.
abstract: During a 3-year period, "Campylobacter upsaliensis" was isolated from 99 patients. Phenotypic characterization and numerical analysis of protein electrophoregrams showed evidence that "C. upsaliensis" is a distinct Campylobacter species with unique characteristics. The MBCs of 13 antibiotics were determined. In general, these organisms were highly susceptible to drugs that were present in the selective isolation media, making none of the available selective media suitable for the isolation of "C. upsaliensis." Ten strains were found to be resistant to erythromycin (MBCs, greater than or equal to 12.50 mg/liter). Plasmid DNA was detectable in 89 of the 99 strains; 16 plasmid profiles could be identified. Plasmid pattern 16, containing four plasmids of 52, 32, 5.5, and 2.6 megadaltons, represented 60.7% of the plasmid-containing strains. None of the "C. upsaliensis" strains could be agglutinated with antisera against heat-labile antigens from C. jejuni, C. coli, or C. laridis. "C. upsaliensis" was found to be susceptible to serum killing and was readily phagocytized by human polymorphonuclear cells.
authors.affiliation: World Health Organization Collaborating Centre for Enteric Campylobacter, St. Pieters University Hospital, Brussels, Belgium.
authors.forename: H
authors.initials: H
authors.lastname: Goossens
authors.forename: B
authors.initials: B
authors.lastname: Pot
authors.forename: L
authors.initials: L
authors.lastname: Vlaes
authors.forename: C
authors.initials: C
authors.lastname: Van den Borre
authors.forename: R
authors.initials: R
authors.lastname: Van den Abbeele
authors.forename: C
authors.initials: C
authors.lastname: Van Naelten
authors.forename: J
authors.initials: J
authors.lastname: Levy
authors.forename: H
authors.initials: H
authors.lastname: Cogniau
authors.forename: P
authors.initials: P
authors.lastname: Marbehant
authors.forename: J
authors.initials: J
authors.lastname: Verhoef
mesh_terms: D001426:Bacterial Proteins / Q000302:isolation & purification; D001770:Blood Bactericidal Activity; D002167:Campylobacter / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D002169:Campylobacter Infections / Q000382:microbiology; D003967:Diarrhea / Q000382:microbiology; D004352:Drug Resistance, Microbial; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000382:microbiology*; D006801:Humans; D010587:Phagocytosis; D010641:Phenotype; D010957:Plasmids; D012703:Serotyping; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins