Persistent effect of TGF-beta 1 on extracellular matrix gene expression in human dermal fibroblasts.
TGF-beta, a potent inducer of the extracellular matrix, is also known to stimulate its own synthesis. In this report we have analyzed long term effects of TGF-beta 1 on its own expression and on the expression of extracellular matrix genes. We demonstrated that 24 hours of incubation of human dermal fibroblasts with TGF-beta 1 (1 ng/ml) under serum free conditions resulted in an elevated expression of TGF-beta 1, collagen alpha 2(I) and fibronectin mRNAs that persisted at least 96 hours after removal of TGF-beta 1. These data suggest the possibility of persistent in vivo activation of target cells following exposure to TGF-beta 1.
Ishikawa
O
O
Department of Medicine, Medical University of South Carolina, Charleston.
Yamakage
A
A
LeRoy
E C
EC
Trojanowska
M
M
D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism*; D005347:Fibroblasts; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis*; D012867:Skin / Q000187:drug effects / Q000378:metabolism*; D015291:Transforming Growth Factors / Q000235:genetics / Q000494:pharmacology*
D012333:RNA, Messenger; D015291:Transforming Growth Factors