Developmental expression of neuronal calmodulin-binding proteins in rat brain.
The developmental patterns of calmodulin-binding proteins (CaM-BPS) in rat brain were examined using biotinylated calmodulin overlays of one- and two-dimensional gels. Hippocampus showed the earliest onset of CaM-BP expression (postnatal day 5; PND5), followed by cerebral cortex and striatum, both of which had detectable levels of CaM-BPs by PND7. Cerebellum had the latest onset of CaM-BP expression; CaM-BPs were not detectable until PND9. Very few CaM-BPs were present in brain before PND5 and all regions reached near adult levels by PND20. However, several unique CaM-BPs were seen in embryonic brain and these proteins may have an important role in developing neurons. These data suggest an orderly, complex expression of CaM-BPs which increases during times of synaptogenesis and synaptic maturation.
Polli
J W
JW
Department of Pharmacology, Milton S. Hershey Medical Center, Pennsylvania State University College of Medicine, Hershey 17033.
Patanow
C M
CM
Billingsley
M L
ML
D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D002148:Calmodulin-Binding Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D002148:Calmodulin-Binding Proteins