Preliminary X-ray crystallographic analysis of holotoxin from Bordetella pertussis.
Pertussis (whooping cough) is a serious infectious disease caused by the bacterium Bordetella pertussis. One of the major virulence factors is a protein known as pertussis toxin, which is composed of six subunits, with a total molecular weight of 106,000. Enzymatic transfer of ADP-ribose from NAD to a family of GTP-binding proteins is effected by the largest subunit (S1 or the A monomer), while binding of host cells and entry of S1 to the interior is a function of the other subunits (the B oligomer). The holotoxin crystallizes in the orthorhombic space group P2(1)2(1)2(1), with unit cell dimensions a = 98.4 A, b = 164.2 A and c = 195.2 A. The crystals are suitable for high-resolution X-ray diffraction analysis.
Raghavan
M
M
Department of Chemistry, Princeton University, NJ 08544.
Gotto
J W
JW
Scott
J V
JV
Schutt
C E
CE
D001886:Bordetella pertussis / Q000032:analysis* / Q000254:growth & development; D006027:Glycosides / Q000032:analysis*; D037342:Pertussis Toxin; D010781:Photography; D011487:Protein Conformation; D014315:Triterpenes / Q000032:analysis*; D010566:Virulence Factors, Bordetella / Q000032:analysis; D014961:X-Ray Diffraction
D006027:Glycosides; D014315:Triterpenes; D010566:Virulence Factors, Bordetella; C001883:stichoposide; D037342:Pertussis Toxin