title: In vitro characterization of two different Phycomyces blakesleeanus mutants with impaired phytoene desaturation.
abstract: In vitro phytoene desaturation was investigated in two Phycomyces blakesleeanus mutants, C5 and S442, in which phytoene is accumulated instead of beta-carotene. For strain C5 but not strain S442 the phenotypic block of phytoene conversion could be overcome in vitro by the addition of Tween 40. Immunodetection of phytoene desaturase revealed in all cases the presence of a 40-kilodalton protein.
authors.affiliation: Lehrstuhl für Physiologie und Biochemie der Pflanzen, Universität Konstanz, Federal Republic of Germany.
authors.forename: A
authors.initials: A
authors.lastname: Schmidt
authors.forename: G
authors.initials: G
authors.lastname: Sandmann
mesh_terms: D001711:Biotransformation; D002338:Carotenoids / Q000378:metabolism*; D007118:Immunoassay; D008970:Molecular Weight; D009090:Mucorales / Q000235:genetics*; D009154:Mutation; D010088:Oxidoreductases / Q000032:analysis; D010641:Phenotype; D010800:Phycomyces / Q000235:genetics* / Q000378:metabolism; D013045:Species Specificity
chemical_list: D002338:Carotenoids; C100185:(all-E) phytoene; D010088:Oxidoreductases; C022398:phytoene dehydrogenase