Dynamics in synthetic oligonucleotides. A solid-state deuterium NMR study.
Solid-state 2H NMR spectroscopy was used to investigate dynamics in the [methyl-2H]thymidine-labeled oligonucleotide [d(CGCGAAT*T*CGCG)]2. Quadrupole echo line shapes, spin-lattice relaxation, and quadrupolar echo decay rates were investigated as a function of hydration W (moles of water/moles of nucleotide) between 0 and approximately 30. The amplitude of the base motion, modeled as a fast four-site libration, or diffusion in a cone, increased slightly with higher levels of hydration. A slower component of motion about the helix axis appears at W approximately 10 and increases in rate and amplitude, leading to the intermediate rate line shape observed at W approximately 21.
Alam
T M
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Department of Chemistry, University of Washington, Seattle 98195.
Drobny
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D003903:Deuterium; D007553:Isotope Labeling / Q000379:methods; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008433:Mathematics; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis
D009838:Oligodeoxyribonucleotides; D003903:Deuterium