title: [Electroelution of protein fractions from a polyacrylamide gel].
abstract: A method for electroelution of protein fractions from polyacrylamide gel and device for performing such a process have been developed. The application of two tris-glycine buffers with the low and high ionic strength, pH 9.0-9.2 provides a concentration of protein simultaneously to extraction from the gel. The duration of elution is in the range of 1-3 hours and depends on the protein mobility. The effectiveness of the system is demonstrated for disc-electrophoretic separation and electrophoresis in slab gel in the presence of SDS. The maximal amount of pure protein fraction obtained is about 4.5-5.0 mg. The method may be useful especially for the fractionation of limited quantities of protein samples.
authors.forename: M D
authors.initials: MD
authors.lastname: Lutsyk
authors.forename: P S
authors.initials: PS
authors.lastname: Oleshko
authors.forename: A S
authors.initials: AS
authors.lastname: Vovkanych
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods*; D011506:Proteins / Q000302:isolation & purification*
chemical_list: D011506:Proteins
vernacular_title: Elektroeliuiuvannia bilkovykh fraktsiĭ z poliakrylamidnogo geliu.