title: [The quantitative determination of Bacillus thuringiensis beta-exotoxin in insecticidal biopreparations].
abstract: A method of quantitative determination of beta-exotoxin content in liquid and dry bioformulations has been developed. The method includes a thin-layer chromatography to isolate beta-exotoxin from accompanying nucleotides, the further desorption of a single beta-exotoxin spot by water and to carry out spectrophotometry at 259 and 330 nm. beta-exotoxin content in industrial formulations bitoxibacillin and turingin I has been determined. The results obtained correspond to the NMR 1H spectroscopy data within the experimental errors. The relative error is 1-2%. The method sensitivity of 0.05 mg/ml. beta-exotoxin content at biotechnological stages of bitoxibacillin production has been determined.
authors.forename: E I
authors.initials: EI
authors.lastname: Efimtsev
authors.forename: G P
authors.initials: GP
authors.lastname: Burov
authors.forename: A A
authors.initials: AA
authors.lastname: Solomin
mesh_terms: D001413:Bacillus thuringiensis; D002855:Chromatography, Thin Layer; D005098:Exotoxins / Q000032:analysis*; D007306:Insecticides / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D009930:Organic Chemicals; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005098:Exotoxins; D007306:Insecticides; D009930:Organic Chemicals; C021633:Bitoxibacillin
vernacular_title: Kolichestvennoe opredelenie beta-ékzotoksina Bacillus thuringiensis v insektitsidnykh biopreparatakh.