[The results of using the molecular hybridization of nucleic acids in the complex study of nasopharyngeal smears from patients with influenza and other acute respiratory diseases].
A complex study of samples obtained from patients with influenza and other acute respiratory diseases has revealed that the laboratory methods used in this study can be rated in the following order according to their sensitivity: isolation of the virus in chick embryos, analysis of seroconversions in the hemagglutination inhibition test, immunofluorescent determination of viral antigens, determination of viral antigens by enzyme immunoassay (EIA), detection of RNA-containing viral structures by means of molecular hybridization. From the point of view of the possibility of documenting influenza A in patients, the best results are achieved by the combination of molecular hybridization and EIA techniques: 90% and more of all cases. A rational scheme for the examination of samples obtained from patients with a view to epidemiological study, including both traditional and new rapid diagnostic methods, is proposed.
Pliusnin
A Z
AZ
Nolandt
O V
OV
Lipina
N V
NV
Somnina
A A
AA
Zibina
E A
EA
Zhamsrangiĭn
M
M
Luzianina
T Ia
TIa
Noskov
F S
FS
D000208:Acute Disease; D000328:Adult; D002648:Child; D004196:Disease Outbreaks; D005069:Evaluation Studies as Topic; D006801:Humans; D009980:Influenza A virus / Q000235:genetics* / Q000302:isolation & purification; D007251:Influenza, Human / Q000175:diagnosis* / Q000235:genetics; D009305:Nasopharynx / Q000382:microbiology*; D009693:Nucleic Acid Hybridization / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics*; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000235:genetics; D012698:Serologic Tests / Q000379:methods
D012367:RNA, Viral
Rezul'taty ispol'zovaniia molekuliarnoĭ gibridizatsii nukleinovykh kislot v kompleksnom issledovanii nosoglotochnykh smyvov ot bol'nykh grippom i drugimi ostrymi respiratornymi zabolevaniiami.