Quantitative determination of terbutaline and orciprenaline in human plasma by gas chromatography/negative ion chemical ionization/mass spectrometry.
A method for the determination of unconjugated terbutaline and orciprenaline in human plasma is described. The assay is based on stable isotope dilution gas chromatography/negative ion chemical ionization/mass spectrometry. An inexpensive and rapid method for preparation of stable isotope labelled analogues as well as their use in quantitative gas chromatography/mass spectrometry is shown. A highly efficient sample work-up procedure with product recoveries of more than 95% is presented. The method developed permits quantitative measurement of terbutaline and orciprenaline in human plasma down to 100 pg ml-1, using 1 ml of sample. Plasma levels of terbutaline after oral administration of 5 mg of terbutaline sulphate were estimated.
Leis
H J
HJ
Univ. Kinderklinik, Dept. Mass Spectrometry, Graz, Austria.
Gleispach
H
H
Nitsche
V
V
Malle
E
E
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D066298:In Vitro Techniques; D009921:Metaproterenol / Q000097:blood*; D013726:Terbutaline / Q000097:blood*
D009921:Metaproterenol; D013726:Terbutaline