Structure of gene coding for rat group II phospholipase A2.
The gene coding for rat group II phospholipase A2 was isolated from a rat genomic library by using the cDNA for rat platelet phospholipase A2 as a hybridization probe. The rat group II phospholipase A2 gene spanned about 3.5 kilobase pairs and consisted of five exons. Southern blot analysis revealed that a single copy of this gene exists in the rat haploid genome. A TATA-like sequence and two AP-2 binding site-like loci were found upstream from the tentatively identified transcription initiation site.
Komada
M
M
Faculty of Pharmaceutical Science, University of Tokyo, Japan.
Kudo
I
I
Inoue
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA / Q000302:isolation & purification; D005091:Exons; D015870:Gene Expression; D015723:Gene Library; D008969:Molecular Sequence Data; D010740:Phospholipases / Q000235:genetics*; D010741:Phospholipases A / Q000235:genetics*; D054467:Phospholipases A2; D051381:Rats; D015183:Restriction Mapping; D013154:Spleen / Q000201:enzymology
D004247:DNA; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2