title: Transcriptional regulation of an aldolase gene in the regenerating rat liver.
abstract: The relative abundance of rat aldolase A, B, C, alpha-fetoprotein, and albumin mRNAs was determined by Northern hybridization during liver regeneration after partial hepatectomy. Aldolase A mRNA increased more than 10-fold on the 3rd day after partial resection compared with that of normal adult rat liver. S1 analysis revealed that three species of aldolase A mRNAs (mRNA I, II & III) reappeared. However, transcriptional rate of aldolase A mRNA did not change at all during the regeneration. In contrast, aldolase B, aldolase C and albumin mRNAs did not change at all. These findings suggest that the differentiated hepatocyte maintains a differentiated state during the liver regeneration as seen in aldolase B, whereas "oncofetal" isozymes such as aldolase A resurge after partial hepatectomy under the control of post-transcriptional mechanism.
authors.affiliation: Department of Biochemistry, Saga Medical School, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Motomura
authors.forename: T
authors.initials: T
authors.lastname: Mukai
authors.forename: I
authors.initials: I
authors.lastname: Ozaki
authors.forename: K
authors.initials: K
authors.lastname: Joh
authors.forename: Y
authors.initials: Y
authors.lastname: Arai
authors.forename: T
authors.initials: T
authors.lastname: Sakai
authors.forename: K
authors.initials: K
authors.lastname: Hori
mesh_terms: D000418:Albumins / Q000235:genetics; D000818:Animals; D015152:Blotting, Northern; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics*; D008115:Liver Regeneration / Q000235:genetics*; D008297:Male; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014158:Transcription, Genetic
chemical_list: D000418:Albumins; D012333:RNA, Messenger; D005634:Fructose-Bisphosphate Aldolase