Sustained stimulation of aldosterone production by angiotensin II is potentiated by nickel.
Angiotensin-induced aldosterone production by superfused adrenal glomerulosa cells was potentiated by Ni2+ (0.1 mM), added either at the onset of stimulation with angiotensin II or 1 h later. Nickel did not influence the effect of adrenocorticotropic hormone or potassium on aldosterone production. Nickel failed to modify angiotensin-induced changes in phospholipid metabolism or the formation of inositol phosphates and slightly reduced the enhancement of 45Ca influx. Uptake of Ni2+ into glomerulosa cells was increased by depolarization in a dihydropyridine-insensitive manner. Because nickel selectively potentiates the sustained phase of the response to a calcium-mobilizing hormone, it may serve as a suitable tool in elucidating the signal transduction process during the sustained phase of stimulation.
Spät
A
A
Department of Physiology, Semmelweis University Medical School, Budapest, Hungary.
Balla
I
I
Balla
T
T
Enyedi
P
P
Hajnóczky
G
G
Rohács
T
T
D000450:Aldosterone / Q000096:biosynthesis*; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D003035:Cobalt / Q000494:pharmacology; D005260:Female; D066298:In Vitro Techniques; D007295:Inositol Phosphates / Q000378:metabolism; D007700:Kinetics; D008297:Male; D009532:Nickel / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D015384:Zona Glomerulosa / Q000187:drug effects / Q000378:metabolism*
D007295:Inositol Phosphates; D000804:Angiotensin II; D003035:Cobalt; D000450:Aldosterone; C022838:nickel chloride; D009532:Nickel; C018021:cobaltous chloride; D002118:Calcium