title: HELP (high efficiency liquid phase) new oligonucleotide synthesis on soluble polymeric support.
abstract: A simple, rapid and high-yielding method for the synthesis of oligonucleotides by the phosphotriesters approach is described. The use of polyethylene glycol (PEG) as soluble polymeric support preserves some convenient features of the solid-phase synthesis with new interesting advantages. Short oligonucleotides in hundred milligrams scale can be obtained from few grams of functionalized PEG.
authors.affiliation: Department of Organic Chemistry, University of Padova, Italy.
authors.forename: G M
authors.initials: GM
authors.lastname: Bonora
authors.forename: C L
authors.initials: CL
authors.lastname: Scremin
authors.forename: F P
authors.initials: FP
authors.lastname: Colonna
authors.forename: A
authors.initials: A
authors.lastname: Garbesi
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D009682:Magnetic Resonance Spectroscopy; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000302:isolation & purification; D011092:Polyethylene Glycols; D012995:Solubility
chemical_list: D009841:Oligonucleotides; D011092:Polyethylene Glycols