Characterization of an endogenous quail intestinal lectin.
Soluble extracts of quail intestine scrapings contain a lectin activity specific for chicken and rabbit trypsinized, glutaraldehyde-fixed erythrocytes. The lectin displayed a specificity for the simple sugar haptens lactose and galactose and for mucin. Quail lectin was purified by affinity chromatography on either asialofetuin- or mucin-Sepharose, followed by DEAE-Sepharose chromatography, and demonstrated an apparent molecular weight of 14,500 on sodium dodecyl sulfate - polyacrylamide gel electrophoresis and a pI of 6.2 upon isoelectric focusing. Immunohistochemical localization of this lectin in the intestine was carried out using polyclonal antibody raised in rabbits and tested for specificity in Western blots. Immunoperoxidase staining for quail lectin showed the lectin to be prominent in secretions at the mucosal surface and in goblet cells.
Fang
R X
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Department of Biological Sciences, University of Calgary, Alta., Canada.
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D000818:Animals; D002846:Chromatography, Affinity; D003370:Coturnix; D007150:Immunohistochemistry; D007422:Intestines / Q000032:analysis*; D007526:Isoelectric Point; D037102:Lectins / Q000302:isolation & purification*; D008970:Molecular Weight
D037102:Lectins