title: Heparin inhibits EcoRI endonuclease cleavage of DNA at certain EcoRI sites.
abstract: Studies presented here demonstrate that heparin inhibits EcoRI endonuclease cleavage of DNA whereas related proteoglycans show no effect. The inhibition occurs at particular EcoRI sites that are near or overlap with palindromic sequences in the murine lambda 5 and Lyt-2 genes. Endogenous heparin from peritoneal mast cells co-isolates with DNA and inhibits digestion of peritoneal cell DNA at the inhibitable sites. Digestion of spleen DNA is inhibited at the same sites when commercial heparin is added prior to digestion. In both cases, the inhibition is abolished by pre-treating the DNA with heparinase. Thus, potential artifacts in restriction fragment length analyses could occur with DNA isolated either from cells that are naturally rich in heparin or from cells to which heparin has been added, e.g., as an anticoagulant.
authors.affiliation: Department of Genetics, Stanford University, CA 94305.
authors.forename: J Z
authors.initials: JZ
authors.lastname: Chen
authors.forename: L A
authors.initials: LA
authors.lastname: Herzenberg
authors.forename: L A
authors.initials: LA
authors.lastname: Herzenberg
mesh_terms: D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism*; D015246:Deoxyribonuclease EcoRI / Q000378:metabolism*; D005091:Exons; D005796:Genes; D006493:Heparin / Q000494:pharmacology*; D008407:Mast Cells / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D010537:Peritoneum; D012091:Repetitive Sequences, Nucleic Acid; D013154:Spleen / Q000378:metabolism
chemical_list: D006493:Heparin; D004247:DNA; D015246:Deoxyribonuclease EcoRI