Compartmentation of 4,6-difluoro-5HT studied by nuclear magnetic resonance in normal and CHS bovine platelets.
A platelet storage pool deficiency (SPD) is present in platelets from cattle with the Chediak-Higashi syndrome (CHS). The most plausible hypothesis for the SPD is that dense granule precursors are simply not formed in CHS megakaryocytes. There is, however, evidence that some recently acquired 5-hydroxytryptamine (5HT) is located in granules and that the granules have an acidic interior. To obtain a greater understanding of the processing of 5HT by SPD platelets, normal and CHS platelets were incubated with 4,6-difluoro-5HT and studied by 19F NMR at 188 mHz. Normal platelets contained 2 compartments for 4,6-difluoro-5HT as indicated by 2 well-developed resonances for each 19F. The resonances were unequal in magnitude. The predominant resonance broadened with lower temperatures and was absent in CHS bovine platelets; it was, therefore, the dense granule compartment. There was only 1 resonance for each 19F in CHS platelets. The chemical shift was identical to the minor resonance, or non-dense granule resonance, found in normal bovine platelets but the resonance width was increased, indicating that some non-dense granule 4,6-difluoro-5HT was in a more restricted environment within CHS platelets than it was in normal platelets.
Meyers
K M
KM
Department of Veterinary and Comparative Anatomy, College of Veterinary Medicine, Washington State University, Pullman.
Costa
J L
JL
Magnuson
J
J
D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood; D002451:Cell Compartmentation / Q000502:physiology*; D002609:Chediak-Higashi Syndrome / Q000097:blood* / Q000150:complications / Q000662:veterinary; D009682:Magnetic Resonance Spectroscopy; D010981:Platelet Storage Pool Deficiency / Q000097:blood / Q000209:etiology / Q000662:veterinary; D012016:Reference Values; D012701:Serotonin / Q000031:analogs & derivatives* / Q000097:blood; D013696:Temperature
D012701:Serotonin; C016378:4,6-difluoroserotonin