Versatile stable isotope technique for the measurement of amino acids and keto acids: comparison with radioactive isotope and its use in measuring in vivo disposal rates.
Tracer methods using both carbon-13 and -14 have been utilized for determination of ovine fetal amino acid disposal and the results compared in seven animals. We infused [1-13C]leucine simultaneously with [1-14C]leucine into the fetal circulation of pregnant sheep chronically catheterized during late gestation. Radioactive and stable isotope enrichments of leucine (Leu) and stable isotope enrichments of ketoisocaproic acid (KIC) in the umbilical artery and vein and the maternal artery and uterine vein were measured. Stable isotope enrichments and concentrations of both Leu and KIC were determined from a single 0.2-ml sample by the use of internal standards and electron ionization GC/MS analysis after a simple isolation and derivatization procedure. The KIC/Leu enrichment ratio was measured for the first time in fetal arterial plasma and was 0.66 +/- 0.05 (SE). Fetal leucine disposal rate was 9.0 +/- 0.5 (SE) micron/min/kg. Disposal rates determined by stable isotopes were not different from those determined by radioactive isotopes. The GC/MS stable isotope method provided higher precision in both leucine concentration and enrichment measurements and has been shown to be a general method for the determination of concentration and isotopic enrichment of other amino acids and their corresponding keto acids. Furthermore, this method is ideally suited to clinical studies where large numbers of samples of rather small volume can easily be studied with a short turnaround time.
Loy
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GL
Department of Obstetrics/Gynecology, University of Colorado Health Sciences Center, Denver 80262.
Quick
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Teng
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Hay
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Fennessey
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D000596:Amino Acids / Q000097:blood*; D000818:Animals; D002208:Caproates / Q000097:blood; D002247:Carbon Isotopes; D002852:Chromatography, Ion Exchange; D005260:Female; D005333:Fetus; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation; D007651:Keto Acids / Q000097:blood*; D007930:Leucine / Q000097:blood; D008722:Methods; D011247:Pregnancy; D012756:Sheep
D000596:Amino Acids; D002208:Caproates; D002247:Carbon Isotopes; D007651:Keto Acids; C001916:alpha-ketocaproic acid; D007930:Leucine