[Usefulness of the Coomassie brilliant blue method for quantitation of total protein in the urine].
Determination of urine total protein concentration employing Macart and Gerbaut method, with rapid assessment of urine protein with Ames strip method was performed and the results were compared. Studied material consisted of a set urines from healthy persons and urines with proteinuria obtained from Outpatient Clinic of Nephrology and Clinic of Metabolic Diseases. In each urine protein was determined using two compared methods. The obtained mean protein concentration in urines with negative deep-stick test was in CBB-SDS method 0.186 +/- 0.112 g/l, whereas for with "trace" protein the CBB-SDS method yields 0.315 +/- 0.159 g/l. For urines with protein concentration assessed as (+), CBB-SDS method yielded 0.558 +/- 0.257 g/l, and for (++), ( ) and ( +) the CBB-SDS method yielded 1.204 +/- 0.454 g/l, 1.647 +/- 0.293 g/l and 2.298 +/- 1.316 g/l respectively. Correlation between protein concentration determined by the CBB-SDS method and deep-stick test Ames method was characterized by r = 0.87 (significant at p less than 0.001). Moreover it was shown correlation between the urine protein concentration determined using both compared methods and number of casts detected in sediment r = 0.33, p less than 0.001 and r = 0.32, p less than 0.001 respectively. On the other hand the Ames Labstix method showed correlation with erythrocytes number, what was not shown using the CBB-SDS method.
Krzemień
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Katedry Diagnostyki Biochemicznej AM w Krakowie.
Klimek
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D004906:Erythrocyte Count; D006801:Humans; D011507:Proteinuria / Q000175:diagnosis*; D012394:Rosaniline Dyes
D012394:Rosaniline Dyes; C004692:coomassie Brilliant Blue
Ocena przydatności metody z błekitem Coomassie Briliant Blue do ilościowego oznaczenia białka w moczu.