Red cell trapping after ischemia and long-term kidney damage. Influence of hematocrit.
The influence of the hematocrit (Hct) on the trapping of red blood cells (RBC) in the renal microvasculature and its effect on the long-term outcome following unilateral ischemia were investigated in the rat. The results showed that an increase in the duration of ischemia increased the RBC trapping, as measured by 51Cr-labeled erythrocytes, in a dose-dependent manner. At normal Hct (46%) the period of ischemia producing half-maximum RBC trapping was 45 minutes, whereas after hemodilution (Hct = 31%) or hemoconcentration (Hct = 60%) the corresponding periods were 80 and 25 minutes, respectively. Regarding the long-term outcome, 45 minutes of ischemia with a normal Hct was associated with a marked decrease in kidney weight, GFR and urine osmolarity after four weeks of recovery, which could be prevented to a large extent by hemodilution. Conversely, with hemoconcentration there was severe damage after only 25 minutes of ischemia. It is suggested that these long-term effects are attributable to RBC trapping in the microvasculature of the outer medulla, which may cause added ischemia in this area of the kidney. It is also suggested that cortical atrophy is secondary to the medullary injury, and is brought about to avoid extensive water and salt losses.
Hellberg
P O
PO
Department of Physiology, University of Uppsala, Sweden.
Bayati
A
A
Källskog
O
O
Wolgast
M
M
D000818:Animals; D001284:Atrophy; D004912:Erythrocytes / Q000502:physiology*; D006400:Hematocrit; D006438:Hemodilution; D007511:Ischemia / Q000097:blood* / Q000473:pathology; D007668:Kidney / Q000098:blood supply* / Q000648:ultrastructure; D008297:Male; D008833:Microcirculation / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D051381:Rats; D011919:Rats, Inbred Strains; D015424:Reperfusion