A rapid method for determining the relationship between cDNA clones.
We describe a technique for rapidly screening the inserts of plasmids for homology to each other by using DNA fragments isolated in agarose gels to probe Southern blots of DNA prepared by the "miniprep" alkaline lysis method. The procedure includes a technique for labeling DNA fragments in agarose gel slices without further purification. The protocol results in a significant savings in time and expense and a considerable increase in fragment yield over methods involving fragment purification from polyacrylamide or agarose gels.
Walker
J A
JA
Pittsburgh Cancer Institute, Basic Research Facility, PA 15213.
Cairns
J S
JS
D001483:Base Sequence; D015139:Blotting, Southern; D004247:DNA / Q000302:isolation & purification*; D015342:DNA Probes; D008722:Methods; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D004247:DNA