title: High hemoglobin mixed disulfide content in hemolysates from stressed shark.
abstract: 1. Hemolysate from heavily stressed smooth hammerhead shark, Sphyrna zygaena, shows three electrophoretic components, SZ I, SZ II and SZ III, whose relative concentrations are 36.4 +/- 6.8, 36.4 +/- 5.0 and 20.8 +/- 5.7%, respectively. After reduction with DTE only SZ I remained. 2. SZ I reacted with glutathione disulfide reconstitute SZ II and SZ III. 3. Non-reduced, DTE-reduced, and denatured hemoglobin were found to have 2.0 +/- 0.4, 3.7 +/- 0.6, and 9.4 +/- 0.7-SH groups, respectively. 4. Erythrocyte non-protein--SH (NPSH), including glutathione present as mixed disulfide with SZ II and SZ III, is 1.7 NPSH/Hb.
authors.affiliation: Departamento Fisiologia, Farmacologia e Biofisica, UFRGS, Porto Alegre, Brasil.
authors.forename: A L
authors.initials: AL
authors.lastname: Dafré
authors.forename: E
authors.initials: E
authors.lastname: Reischl
mesh_terms: D000293:Adolescent; D000818:Animals; D002850:Chromatography, Gel; D004220:Disulfides / Q000032:analysis; D004229:Dithiothreitol / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004592:Electrophoresis, Starch Gel; D006454:Hemoglobins / Q000302:isolation & purification*; D006461:Hemolysis; D006801:Humans; D046911:Macromolecular Substances; D012754:Sharks; D013312:Stress, Physiological / Q000097:blood*
chemical_list: D004220:Disulfides; D006454:Hemoglobins; D046911:Macromolecular Substances; D004229:Dithiothreitol