Production of highly specific anti-testosterone antiserum by an immunotolerance method.
Anti-testosterone antisera were produced by pretreatment of rabbits with 15 alpha-carboxymethyl-5 alpha-dihydrotestosterone linked to a copolymer of D-glutamic acid and D-lysine (D-GL) before immunization with the bovine serum albumin conjugate of 15 alpha- and 15 beta-carboxymethyltestosterone. The specificity for 5 alpha-dihydrotestosterone of the antitestosterone antisera was considerably improved.
Miyake
Y
Y
Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Shigetoshi
M
M
Kojima
M
M
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000918:Antibody Specificity; D007108:Immune Tolerance; D066298:In Vitro Techniques; D011817:Rabbits; D013739:Testosterone / Q000276:immunology*
D000906:Antibodies; D013739:Testosterone