Effect of H2O2 concentration and exposure time on stromal swelling: an epithelial perfusion model.
The corneal epithelium can be affected adversely by some preservatives used in contact lens solutions. To test the effect of H2O2 at the epithelial surface, the rabbit cornea was perfused with various concentrations of H2O2, and the effect on stromal and Anterior Bright Band (ABB) thickness measured for 150 min. The study compares stromal swelling and ABB swelling for a single 10-min pulse with that for a 150-min sustained dose. With the 10-min pulse dose there was no significant swelling up to 235 ppm H2O2, the highest dose tested. With the sustained dose, significant stromal swelling began between 72 and 153 ppm H2O2. These results indicate that residual H2O2 present at the epithelial surface after neutralization in current contact lens disinfection systems does not have a high enough concentration to affect corneal function adversely.
Wilson
G S
GS
University of Alabama, School of Optometry, Birmingham.
Chalmers
R L
RL
D000818:Animals; D002387:Cataract Extraction; D003261:Contact Lenses; D003315:Cornea / Q000187:drug effects*; D003319:Corneal Stroma / Q000187:drug effects*; D004202:Disinfectants / Q000009:adverse effects*; D004305:Dose-Response Relationship, Drug; D004848:Epithelium / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000008:administration & dosage / Q000009:adverse effects*; D008954:Models, Biological; D010477:Perfusion; D011817:Rabbits; D013997:Time Factors
D004202:Disinfectants; D006861:Hydrogen Peroxide