Adenosine activation of a nuclear pool of protein kinase C in rat splenocytes.
The stimulatory effects of adenosine analogues on a nuclear pool of protein kinase C (PKC) were examined using isolated rat splenocyte nuclei. Nuclear receptors met pharmacological criteria of A1 adenosine receptors including a potency profile in which cyclopentyladenosine (CPA), a selective A1 agonist, was more potent than 2-phenylaminoadenosine (2PAA), a selective A2 agonist. The selective A1 receptor agonist N6-1-(phenyl-2R-propyl) adenosine (R-PIA) activated PKC whereas the S diastereomer did not. The adenosine-induced PKC response could be attenuated using a monoclonal antibody to PKC, an A1 receptor antagonist, three known PKC inhibitors and pertussis toxin (PTX). The results suggest that adenosine may exert immunomodulatory effects through the activation of nuclear PKC.
Sauro
M D
MD
Department of Pharmacology and Therapeutics, University of South Florida College of Medicine, Tampa.
Zorn
N E
NE
Olsson
R A
RA
Russell
D H
DH
D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology*; D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology; D002478:Cells, Cultured; D004789:Enzyme Activation; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011983:Receptors, Purinergic / Q000032:analysis*; D013154:Spleen / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure
D011983:Receptors, Purinergic; D011493:Protein Kinase C; D000241:Adenosine