Autocrine growth factor and spontaneous transformation of rat liver epithelial cells.
Liver epithelial cells from a young rat were cultured and passaged for a long period until they showed a malignant transformation (tumorigenicity in athymic mice), to know when an autocrine growth factor is produced in the course of a spontaneous malignant transformation of normal cells in vitro. The cells initially showed a cellular transformation and an increased growth rate, then produced an autocrine growth factor after Passage 55, and finally the cells after Passage 105 showed a malignant transformation (tumorigenicity in vivo). It is suggested that in addition to an autocrine growth factor, some other factors might be related to a malignant transformation (tumorigenicity in vivo).
Narita
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Department of Pathology, Hirosaki University School of Medicine, Japan.
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D006133:Growth Substances / Q000378:metabolism*; D008099:Liver / Q000166:cytology* / Q000378:metabolism / Q000473:pathology; D051381:Rats
D003470:Culture Media; D006133:Growth Substances