Protection of Toxoplasma gondii-infected mice by stearylamine-bearing liposomes.
The cytotoxic activity of stearylamine-bearing liposomes against Toxoplasma gondii (RH strain) was examined. When tachyzoites were treated in vitro with liposomes consisting of 20 mol% stearylamine and 80 mol% phosphatidylcholine (130 micrograms/ml total lipids), more than 95% of the parasites were killed within 90 min. Intraperitoneal injection of 10 mg of 30 mol% stearylamine/70 mol% phosphatidylcholine-liposomes in mice shortly before or after T. gondii challenge afforded protection from death for more than 30 days to 70-80% of the treated mice, whereas all untreated mice succumbed within 9 days. The liposome-injected mice that survived remained symptom-free and behaved normally.
Tachibana
H
H
Department of Parasitology, Tokai University School of Medicine, Kanagawa, Japan.
Yoshihara
E
E
Kaneda
Y
Y
Nakae
T
T
D000588:Amines / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D004337:Drug Carriers; D005260:Female; D008081:Liposomes; D051379:Mice; D008813:Mice, Inbred ICR; D010713:Phosphatidylcholines / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D014122:Toxoplasma / Q000187:drug effects; D014124:Toxoplasmosis, Animal / Q000188:drug therapy* / Q000517:prevention & control
D000588:Amines; D004337:Drug Carriers; D008081:Liposomes; D010713:Phosphatidylcholines; C009317:stearylamine