TGF-beta 1 binding protein: a component of the large latent complex of TGF-beta 1 with multiple repeat sequences.
TGF-beta occurs in a latent complex of high Mr. We report the cDNA cloning and an initial structural and functional characterization of a component of the large latent TGF-beta 1 complex, denoted TGF-beta 1 binding protein (TGF-beta 1-BP). Most of the sequence of fibroblast TGF-beta 1-BP is made up of cysteine-rich repeats of two different kinds; there are 16 EGF-like repeats and three repeats with a distant resemblance to EGF, but of a distinct type hitherto not found in any other protein. beta-hydroxylated asparagine residues were identified in two of the EGF-like repeats. TGF-beta 1-BP purified from human platelets is considerably smaller than the fibroblast form (125-160 kd vs. 170-190 kd), suggesting that there is alternative splicing of the TGF-beta 1-BP gene or that TGF-beta 1-BP undergoes cell-specific proteolysis. TGF-beta 1-BP was found not to bind and inactive TGF-beta 1; its role in the latent complex is discussed.
Kanzaki
T
T
Ludwig Institute for Cancer Research, Biomedical Center, Uppsala, Sweden.
Olofsson
A
A
Morén
A
A
Wernstedt
C
C
Hellman
U
U
Miyazono
K
K
Claesson-Welsh
L
L
Heldin
C H
CH
D000595:Amino Acid Sequence; D000818:Animals; D001216:Asparagine / Q000031:analogs & derivatives / Q000032:analysis; D001483:Base Sequence; D001792:Blood Platelets / Q000378:metabolism; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics* / Q000302:isolation & purification; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D015870:Gene Expression; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D053779:Latent TGF-beta Binding Proteins; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010446:Peptide Fragments / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D012091:Repetitive Sequences, Nucleic Acid; D015291:Transforming Growth Factors / Q000235:genetics*
D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D053779:Latent TGF-beta Binding Proteins; D010446:Peptide Fragments; D012333:RNA, Messenger; C050931:3-hydroxyasparagine; D001216:Asparagine; D015291:Transforming Growth Factors; D004247:DNA