title: Gastrointestinal tissue diagnosis by laser-induced fluorescence spectroscopy at endoscopy.
abstract: An endoscope-compatible, optical fiber system has been developed which can be used to obtain laser-induced fluorescence spectra of mucosal abnormalities during endoscopy in real time. The results of our previous in vitro studies have suggested that laser-induced fluorescence tissue spectra are sufficiently unique that they can be used to accurately diagnose mucosal abnormalities in some systems. To test this hypothesis in vivo, laser-induced fluorescence spectra were obtained during colonoscopy from 31 colonic adenomas, 4 hyperplastic polyps, and 32 examples of normal mucosa in 20 patients. The resulting spectra could be used to correctly differentiate adenomas from normal colonic mucosa and hyperplastic polyps in 97% of the specimens studied with the resulting sensitivity, specificity, and positive predictive value of 100%, 97%, and 94%, respectively. These results, although preliminary in nature, suggest that laser-induced fluorescence spectra can be used in the recognition and differential diagnosis of mucosal abnormalities at endoscopy.
authors.affiliation: Division of Research, Cleveland Clinic Foundation, Ohio 44195.
authors.forename: R M
authors.initials: RM
authors.lastname: Cothren
authors.forename: R
authors.initials: R
authors.lastname: Richards-Kortum
authors.forename: M V
authors.initials: MV
authors.lastname: Sivak
authors.forename: M
authors.initials: M
authors.lastname: Fitzmaurice
authors.forename: R P
authors.initials: RP
authors.lastname: Rava
authors.forename: G A
authors.initials: GA
authors.lastname: Boyce
authors.forename: M
authors.initials: M
authors.lastname: Doxtader
authors.forename: R
authors.initials: R
authors.lastname: Blackman
authors.forename: T B
authors.initials: TB
authors.lastname: Ivanc
authors.forename: G B
authors.initials: GB
authors.lastname: Hayes
mesh_terms: D000236:Adenoma / Q000175:diagnosis*; D003113:Colonoscopy; D003937:Diagnosis, Differential; D006801:Humans; D007414:Intestinal Neoplasms / Q000175:diagnosis*; D007417:Intestinal Polyps / Q000175:diagnosis*; D007422:Intestines / Q000473:pathology*; D007834:Lasers; D013050:Spectrometry, Fluorescence / Q000379:methods*