Isolation and characterization of TGF-beta 2 and TGF-beta 5 from medium conditioned by Xenopus XTC cells.
TGF-beta 2 and -beta 5 have been purified from medium conditioned by Xenopus cultured cells (XTC) and identified based on their N-terminal amino acid sequence analysis and biological activity. When applied in high concentrations, Xenopus TGF-beta 2, like porcine TGF-beta 2, induces expression of mesodermal markers from cultured Xenopus ectodermal explants, whereas TGF-beta 5 is inactive in this assay. However, the TGF-beta 's could be separated from the major mesoderm-inducing activity present in XTC medium. Xenopus TGF-beta 2 and -beta 5 are approximately equivalent to TGF-beta 1 in their abilities to inhibit the growth of mink lung CCL-64 cells, induce anchorage-independent growth of rat NRK cells, inhibit the proliferation and antibody secretion of human B-lymphocytes, and stimulate chemotaxis of human monocytes. These data establish the functional activity of TGF-beta 5 and suggest that more complex multicellular systems, in contrast to most isolated cells, discriminate between the different TGF-beta s.
Roberts
A B
AB
Laboratory of Chemoprevention, National Cancer Institute, National Institute of Child Health and Human Development, Bethesda, Maryland 20892.
Rosa
F
F
Roche
N S
NS
Coligan
J E
JE
Garfield
M
M
Rebbert
M L
ML
Kondaiah
P
P
Danielpour
D
D
Kehrl
J H
JH
Wahl
S M
SM
D000595:Amino Acid Sequence; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002633:Chemotaxis / Q000187:drug effects; D003429:Cross Reactions; D003470:Culture Media; D007120:Immunochemistry; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D015291:Transforming Growth Factors / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D014981:Xenopus
D003470:Culture Media; D015291:Transforming Growth Factors