title: Isolation and characterization of TGF-beta 2 and TGF-beta 5 from medium conditioned by Xenopus XTC cells.
abstract: TGF-beta 2 and -beta 5 have been purified from medium conditioned by Xenopus cultured cells (XTC) and identified based on their N-terminal amino acid sequence analysis and biological activity. When applied in high concentrations, Xenopus TGF-beta 2, like porcine TGF-beta 2, induces expression of mesodermal markers from cultured Xenopus ectodermal explants, whereas TGF-beta 5 is inactive in this assay. However, the TGF-beta 's could be separated from the major mesoderm-inducing activity present in XTC medium. Xenopus TGF-beta 2 and -beta 5 are approximately equivalent to TGF-beta 1 in their abilities to inhibit the growth of mink lung CCL-64 cells, induce anchorage-independent growth of rat NRK cells, inhibit the proliferation and antibody secretion of human B-lymphocytes, and stimulate chemotaxis of human monocytes. These data establish the functional activity of TGF-beta 5 and suggest that more complex multicellular systems, in contrast to most isolated cells, discriminate between the different TGF-beta s.
authors.affiliation: Laboratory of Chemoprevention, National Cancer Institute, National Institute of Child Health and Human Development, Bethesda, Maryland 20892.
authors.forename: A B
authors.initials: AB
authors.lastname: Roberts
authors.forename: F
authors.initials: F
authors.lastname: Rosa
authors.forename: N S
authors.initials: NS
authors.lastname: Roche
authors.forename: J E
authors.initials: JE
authors.lastname: Coligan
authors.forename: M
authors.initials: M
authors.lastname: Garfield
authors.forename: M L
authors.initials: ML
authors.lastname: Rebbert
authors.forename: P
authors.initials: P
authors.lastname: Kondaiah
authors.forename: D
authors.initials: D
authors.lastname: Danielpour
authors.forename: J H
authors.initials: JH
authors.lastname: Kehrl
authors.forename: S M
authors.initials: SM
authors.lastname: Wahl
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002633:Chemotaxis / Q000187:drug effects; D003429:Cross Reactions; D003470:Culture Media; D007120:Immunochemistry; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D015291:Transforming Growth Factors / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D014981:Xenopus
chemical_list: D003470:Culture Media; D015291:Transforming Growth Factors