[Studies on elimination of mycoplasmas from cell line cultures].
Mycoplasmas were effectively eliminated from contaminated LTK and CHO cell lines by means of the antibiotic Tiamutin (10 micrograms/ml), while Kanamycin and Gentamycin were less potential. An elimination procedure which involved the consecutive treatment of the cell cloning over a periods of 21-28 days was adopted. This procedure was effective when applied to cell lines contaminated with unidentified and partially non-cultivable strains of mycoplasmas which could be detected by electronic microscope.
Zhang
H J
HJ
Shanghai Institute of Biological Products, Ministry of Public Health.
Shi
Q M
QM
Hu
Q Q
QQ
He
B G
BG
Wang
Y
Y
Lu
X Y
XY
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002460:Cell Line; D002478:Cells, Cultured; D006224:Cricetinae; D004224:Diterpenes / Q000494:pharmacology; D005260:Female; D009174:Mycoplasma / Q000187:drug effects* / Q000302:isolation & purification; D010053:Ovary / Q000166:cytology
D000900:Anti-Bacterial Agents; D004224:Diterpenes; C014224:tiamulin