Affinity chromatography of fibroblast growth factors on substituted polystyrene.
The heparin-binding growth factors aFGF and bFGF (acidic and basic fibroblast growth factor) from crude bovine brain extract were co-eluted with purified [125I]aFGF and/or [125I]bFGF as tracers from heparin-Sepharose and from several insoluble substituted polystyrenes used as stationary phases in low-pressure affinity chromatography. The ability of the resins to isolate FGFs was determined by measuring the eluted radioactivity. It was demonstrated that the various substituted polystyrene resins retain [125I]aFGF and [125I]bFGF with different specificities according to the chemical nature of the substituted groups bound to the polystyrene support. Bifunctional resins substituted with sulphonate and phenylalanine sulphamide groups adsorbed both [125I]aFGF and [125I]bFGF whereas bifunctional resins substituted with sulphonate and sulphamide serine adsorbed only [125I]bFGF. These stationary phases could be adapted to high-performance affinity chromatography and used to isolate growth factors of the FGF family.
Dourges
M A
MA
Laboratoire de Recherches sur les Macromolécules, URA O502 CNRS, Université Paris-Nord, Villetaneuse, France.
Gulino
D
D
Courty
J
J
Badet
J
J
Barritault
D
D
Jozefonvicz
J
J
D000818:Animals; D001921:Brain / Q000166:cytology; D001923:Brain Chemistry; D002417:Cattle; D002457:Cell Extracts / Q000032:analysis; D002846:Chromatography, Affinity / Q000379:methods*; D005346:Fibroblast Growth Factors / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D011137:Polystyrenes / Q000378:metabolism
D002457:Cell Extracts; D011137:Polystyrenes; D005346:Fibroblast Growth Factors