Protein tyrosine phosphorylation is induced in murine B lymphocytes in response to stimulation with anti-immunoglobulin.
Activation of both T and B lymphocytes through their membrane receptors for antigen is known to induce breakdown of inositol phospholipids. In addition, T cell activation by antigen is accompanied by increased protein tyrosine phosphorylation of components of the T cell antigen receptor. We now provide evidence that B cell activation through membrane immunoglobulin is also coupled to stimulation of protein tyrosine kinase activity. One potential candidate for a B lymphocyte protein tyrosine kinase is an 80 kd molecule that is itself phosphorylated at tyrosine residues in response to stimulation with anti-immunoglobulin antibodies.
Campbell
M A
MA
Molecular Biology and Virology Laboratory, Salk Institute, San Diego, CA 92138.
Sefton
B M
BM
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000201:enzymology / Q000276:immunology*; D002460:Cell Line; D002846:Chromatography, Affinity; D004261:DNA Replication; D005260:Female; D007136:Immunoglobulins / Q000276:immunology*; D015847:Interleukin-4 / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D010750:Phosphoproteins / Q000302:isolation & purification; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D013154:Spleen / Q000276:immunology; D014443:Tyrosine
D000906:Antibodies; D000911:Antibodies, Monoclonal; D007136:Immunoglobulins; D008070:Lipopolysaccharides; D010750:Phosphoproteins; D015847:Interleukin-4; D014443:Tyrosine; D011505:Protein-Tyrosine Kinases