Developmental changes in testicular inhibin and androgen-binding protein during sexual maturation in the cynomolgus monkey, Macaca fascicularis.
Inhibin was measured by RIA in testicular extracts and plasma of cynomolgus monkeys during four stages of sexual maturation. Immunoactive inhibin levels were compared to those of another Sertoli cell secreted protein, androgen-binding protein (ABP). ABP steroid-binding (bioactive) activity was measured in testes and epididymal segments using the radiolabeled ligand [3H]dihydrotestosterone (DHT). Testicular immunoreactive inhibin concentrations were maximal in late prepubertal monkeys, 2.5-3.5 yr old, while the total testicular content of inhibin progressively increased with age into adulthood. Bioactive testicular ABP concentrations were maximal during the pubertal period of the cynomolgus monkey (3.5-4.0 yr old), while the total ABP content of the testes also increased with sexual maturation. Mean (+/- SE) plasma concentrations of inhibin and testosterone (T) in adults, 6-8 yr old (17.72 +/- 3.5 microliters inhibin equivalents/ml and 7.07 +/- 2.45 ng/ml T, respectively), were significantly higher (P less than 0.05 and P less than 0.001, respectively) than those in early prepubertal, juvenile monkeys, aged 1.5-2.5 yr (5.85 +/- 2.1 microliters inhibin equivalents/ml and 0.27 +/- 0.02 ng/ml T). The increased plasma levels of inhibin and T in adults were associated, respectively, with the increased inhibin and androgen contents of the testes in these same animals. The developmental changes in testicular steady state mRNA concentrations for the inhibin alpha-, beta A-, and beta B-subunits as well as ABP were examined during sexual maturation by Northern blot analysis using heterologous human cDNA probes. Densitometric analysis of the autoradiograms revealed that the inhibin alpha-subunit mRNA concentrations were higher than those of inhibin-beta A and -beta B and ABP mRNA during all stages of pubertal development. Although the relative concentrations of each inhibin subunit mRNA were decreased in the adult animals relative to those in the juvenile monkeys, the total amount of steady state mRNA for the subunits was greater than that in the immature animals. A similar situation existed for the ABP mRNA.(ABSTRACT TRUNCATED AT 400 WORDS)
Keeping
H S
HS
Department of Medicine, Montefiore Hospital, Pittsburgh, Pennsylvania.
Winters
S J
SJ
Attardi
B
B
Troen
P
P
D000375:Aging / Q000378:metabolism; D000727:Androgen-Binding Protein / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015342:DNA Probes; D013196:Dihydrotestosterone / Q000378:metabolism; D004822:Epididymis / Q000378:metabolism; D007265:Inhibins / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D008252:Macaca fascicularis; D008297:Male; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism; D011863:Radioimmunoassay; D012741:Sexual Maturation / Q000502:physiology*; D013737:Testis / Q000378:metabolism*; D013739:Testosterone / Q000097:blood
D000727:Androgen-Binding Protein; D015342:DNA Probes; D012333:RNA, Messenger; D013196:Dihydrotestosterone; D013739:Testosterone; D007265:Inhibins