Class IV Ca2+ antagonists do not affect lipid peroxidation in singlet oxygen challenged cardiomyocytes.
The effects of various Ca2+ antagonists on lipid peroxidation in singlet O2-challenged isolated cardiomyocytes from adult rat heart were investigated. Singlet O2-challenged untreated cells all hypercontracted as a consequence of Ca2+ overload and produced 463.6 +/- 143.6 nM malondialdehyde (MDA; mean +/- SD, n = 8). Protective Ca2+ antagonists reduced the amount of damaged cells, but did generally not affect MDA production. On the other hand, free radical scavengers and antioxidants displayed a good correlation between number of protected cells and MDA produced. It is concluded that flunarizine-like Ca2+ antagonists protect cells against Ca2+ overload without, however, interfering with peroxidative processes.
Ver Donck
L
L
Life Sciences Department, Janssen Research Foundation, Beerse, Belgium.
Verellen
G
G
Vandewiele
R
R
De Clerck
F
F
Borgers
M
M
D000818:Animals; D052160:Benzothiazoles; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002469:Cell Separation; D002936:Cinnarizine / Q000494:pharmacology; D004110:Diltiazem / Q000494:pharmacology; D005444:Flunarizine / Q000494:pharmacology; D008013:Lidoflazine / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects*; D008315:Malondialdehyde / Q000378:metabolism; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism; D009529:Nicardipine / Q000494:pharmacology; D010100:Oxygen / Q000494:pharmacology*; D010880:Piperidines / Q000494:pharmacology; D051381:Rats; D013844:Thiazoles / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
D052160:Benzothiazoles; D002121:Calcium Channel Blockers; D010880:Piperidines; D013844:Thiazoles; D002936:Cinnarizine; D008315:Malondialdehyde; C054551:R 56865; D014700:Verapamil; D009529:Nicardipine; D004110:Diltiazem; D008013:Lidoflazine; D005444:Flunarizine; D010100:Oxygen