Differences between the venoms of two sub-species of Russell's viper: Vipera russelli pulchella and Vipera russelli siamensis.
Ion exchange chromatography was carried out using venoms obtained from two sub-species of Russell's viper; V. russelli siamensis from Burma and V. russelli pulchella from Sri Lanka. Differences were observed in the elution position of venom components having haemolytic and procoagulant activity but not those causing fibrinolysis. Only the V. russelli siamensis venom exhibited any platelet aggregating activity. The Indian (Haffkine) polyspecific and the Burmese (Burma Pharmaceutical Industries) monospecific antivenoms, when used in cross immunoelectrophoresis against the two venoms, revealed differences in the number and/or intensity of the precipitin bands present. An important functional consequence of this was that the Burmese antivenom did not neutralize the haemolytic activity of the V. russelli pulchella venom in vitro and would thus probably not be effective in treating this consequence of envenoming by Russell's viper in Sri Lanka. Differences in the composition and the clinical effects of the two venoms emphasizes the importance of using venom from the local snake for antivenom production if optimal clinical efficacy is to be achieved.
Woodhams
B J
BJ
Department of Haematology, Royal Free Hospital, London, U.K.
Wilson
S E
SE
Xin
B C
BC
Hutton
R A
RA
D002852:Chromatography, Ion Exchange; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D007122:Immunoelectrophoresis; D010974:Platelet Aggregation / Q000187:drug effects; D013045:Species Specificity; D014757:Viper Venoms / Q000032:analysis* / Q000633:toxicity
D014757:Viper Venoms