Relationship between post-heparin plasma lipases, triglycerides and high density lipoproteins in normal subjects.
The relationship between plasma lipids and lipoproteins and the lipolytic activities of post-heparin plasma lipoprotein lipase (LpL) and hepatic-triglyceride lipase (H-TGL) was examined in normal subjects. Seven males and six females were given a high fat diet [15% carbohydrate (CARB), 65% fat, 20% protein] for 2 weeks followed by 4 weeks of a high CARB diet (65% CARB, 15% fat, 20% protein). Changes in plasma triglyceride concentrations associated with diet were negatively correlated with changes in HDL-C (r = -0.533, P less than 0.001) and the HDL subfraction HDL2b (r = -0.308, P less than 0.001). The activity of LpL in post-heparin plasma was positively correlated with changes in plasma HDL-C (r = 0.668, P less than 0.001) and HDL2b (r = 0.457, P less than 0.001), and negatively with plasma triglycerides (r = -0.546, P less than 0.001). Changes in H-TGL activity were negatively correlated with changes in HDL2b (r = -231, P less than 0.05) and positively correlated with HDL-C (r = 0.326, P less than 0.01). These results in normal subjects provide further evidence that LpL and H-TGL are important enzymes in the metabolism of plasma lipoproteins and that changes in their activities contribute to plasma lipid and lipoprotein concentrations.
Jackson
R L
RL
Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, Ohio.
Yates
M T
MT
McNerney
C A
CA
Kashyap
M L
ML
D000328:Adult; D008076:Cholesterol, HDL / Q000097:blood; D004032:Diet; D004040:Dietary Carbohydrates / Q000008:administration & dosage; D004041:Dietary Fats / Q000008:administration & dosage; D005260:Female; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D008049:Lipase / Q000097:blood*; D008066:Lipolysis / Q000187:drug effects; D008071:Lipoprotein Lipase / Q000097:blood; D008075:Lipoproteins, HDL / Q000097:blood*; D008297:Male; D014280:Triglycerides / Q000097:blood*
D008076:Cholesterol, HDL; D004040:Dietary Carbohydrates; D004041:Dietary Fats; D008075:Lipoproteins, HDL; D014280:Triglycerides; D006493:Heparin; D008049:Lipase; D008071:Lipoprotein Lipase