Complementary deoxyribonucleic acid cloning of an mRNA encoding transforming growth factor-beta 2 from chicken embryo chondrocytes.
Using a simian transforming growth factor-beta 2 (TGF-beta 2) cDNA probe, we have isolated chicken cDNA clones for TGF-beta 2 from a chicken embryo chondrocyte cDNA library. The predicted precursor protein of chicken TGF-beta 2 is 412 amino acids long, two amino acids shorter than the human form to which it shows 90% identity. Cleavage of the chicken TGF-beta 2 precursor at a pentabasic Arg-Arg-Lys-Lys-Arg site would produce a 112-amino acid processed peptide differing by only one amino acid from the human TGF-beta 2 peptide. In contrast to TGF-beta 3 and 4 mRNAs, TGF-beta 2 mRNA is expressed in both cultured and non-cultured chicken embryo chondrocytes and at higher levels in chicken embryo fibroblasts. In chondrocytes and fibroblasts, the 3.9- and 4.3-kb TGF-beta 2 mRNAs are both expressed at higher levels than the 8-kb TGF-beta 2 mRNA; however, in developing chicken embryos, the level of expression of the 8-kb mRNA is higher than that of the 3.9- and 4.3-kb mRNAs.
Jakowlew
S B
SB
Laboratory of Chemoprevention, National Cancer Institute, NIH, Bethesda, Maryland 20892.
Dillard
P J
PJ
Sporn
M B
MB
Roberts
A B
AB
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002356:Cartilage / Q000378:metabolism*; D002642:Chick Embryo; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D015291:Transforming Growth Factors / Q000235:genetics*
D012333:RNA, Messenger; D015291:Transforming Growth Factors; D004247:DNA