An experimental model of death from anaphylactic shock with compound 48/80 and postmortem changes in levels of histamine in blood.
The present study was made on an experimental animal model of a death from anaphylaxis, in which postmortem changes in levels of histamine and 1-methylhistamine, in whole blood were measured. Instead of the usual immunological method administering compound 48/80, a degranulating agent of mast cell and the effect closely resembling the immuno-reaction, resulted in reliable death in a short time. The animals that died rapidly after the injection of compound 48/80, were found to have large increases in levels of histamine and 1-methylhistamine soon after the administration. These results were similar to the results of injecting histamine exogenously. On the other hand, the animals that died after a longer time showed no increases in levels of those amines within about 24 h, but 24 hours after death histamine levels were only increased tremendously without rise in 1-MHA levels. These phenomena closely resembled those in the control animals that were treated with overdoses of Nembutal.
Hieda
Y
Y
Department of Legal Medicine, Fukuoka University School of Medicine, Japan.
Kageura
M
M
Hara
K
K
Takamoto
M
M
Fukuma
Y
Y
Kashimura
S
S
D000328:Adult; D000707:Anaphylaxis / Q000097:blood / Q000473:pathology; D000818:Animals; D002423:Cause of Death; D004195:Disease Models, Animal; D004285:Dogs; D005260:Female; D006632:Histamine / Q000097:blood* / Q000494:pharmacology; D006801:Humans; D008297:Male; D008761:Methylhistamines / Q000097:blood; D008875:Middle Aged; D010424:Pentobarbital / Q000506:poisoning; D011180:Postmortem Changes; D003189:p-Methoxy-N-methylphenethylamine
D008761:Methylhistamines; D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine; D010424:Pentobarbital