Cobalamin inactivation induces formyltetrahydrofolate synthetase.
Loss of cobalamin function produces profound changes in the metabolism of formate. There is impaired synthesis of formyltetrahydropteroylglutamate synthetase (CHO-H4PteGlu), accumulation of endogenous formate and impaired utilization of [14C]formate. There are contradictory reports on the effect of cobalamin inactivation on CHO-H4PteGlu synthetase. This study confirms a significant increase in synthetase activity following cobalamin inactivation.
Deacon
R
R
MRC Clinical Research Centre, Northwick Park Hospital, Harrow, UK.
Perry
J
J
Lumb
M
M
Chanarin
I
I
D000704:Analysis of Variance; D000818:Animals; D004790:Enzyme Induction / Q000187:drug effects; D005574:Formate-Tetrahydrofolate Ligase / Q000096:biosynthesis*; D008025:Ligases / Q000096:biosynthesis*; D008099:Liver / Q000201:enzymology*; D008297:Male; D009609:Nitrous Oxide / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D014805:Vitamin B 12 / Q000037:antagonists & inhibitors*
D008025:Ligases; D005574:Formate-Tetrahydrofolate Ligase; D009609:Nitrous Oxide; D014805:Vitamin B 12