Determination of biological peptide leukotrienes C4 and D4 by fluorometric detection combined with high-performance liquid chromatography.
A fluorometric procedure combined with high-performance liquid chromatography (HPLC) has been developed to separate and quantitate peptide-leukotrienes (p-LTs) C4 and D4. The method is based on pre-column dansylation of the amino terminus of the p-LTs. Its application for the determination of the amounts of p-LTs C4 and D4 in human blood and sputum is also described. The lower limit of detection of p-LTs with this method is 1 pmol.
Yamashita
Y
Y
Institute of Clinical Medicine, University of Tsukuba, Ibaraki, Japan.
Uchida
Y
Y
Nomura
A
A
Hasegawa
S
S
Kimura
S
S
D002851:Chromatography, High Pressure Liquid; D003619:Dansyl Compounds; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D011863:Radioimmunoassay; D013189:SRS-A / Q000032:analysis* / Q000097:blood; D013183:Sputum / Q000032:analysis
D003619:Dansyl Compounds; D013189:SRS-A