Physical demonstration of a high molecular weight bacteriocin plasmid in Vibrio cholerae by genetic transformation process.
Bacteriocinogeny was transferred at high frequencies from bacteriocinogenic (Bac+) V. cholerae strains to non-bacteriocinogenic (Bac-) recipients in the in situ genetic transformation system on agar surface. DNA extracted from samples of growth of bacteria transformed to Bac+ were obtained at 2 h intervals following contact with the sterile agar surface where the donor had grown previously. This showed acquisition of a high molecular weight plasmid which could be physically demonstrated best in the 6 h sample of the Bac+ transformants; their 4 h samples failed to show this specific plasmid, while it was demonstrable only as a faint band in the 8 h samples.
Basu
S
S
Department of Medical Microbiology & Parasitology, University College of Medicine, Calcutta University.
Chakrabarty
A N
AN
Ganguli
M
M
Dastidar
S G
SG
D001430:Bacteriocins / Q000096:biosynthesis*; D004269:DNA, Bacterial / Q000032:analysis*; D010957:Plasmids; D014169:Transformation, Bacterial; D014734:Vibrio cholerae / Q000235:genetics*
D001430:Bacteriocins; D004269:DNA, Bacterial