An ELISA for quantitation of tetanus toxin.
A sandwich ELISA for quantitation of tetanus toxin was devised using commercially available horse antitetanus antiserum and its HRP-conjugate. With commercial toxoid as a standard the assay had a lower sensitivity of 100 ng/ml and was linear up to 100 micrograms/ml. In terms of Lf equivalents the ELISA assay was linear from 0.02 to 20 Lf/ml. The Lf activity in 20 preparations of tetanus toxin as determined by ELISA was comparable to the activity measured by conventional flocculation tests (r = 0.987).
Pal
A
A
Department of Microbiology, All India Institute of Medical Sciences, New Delhi.
Kumar
R
R
Jailkhani
B L
BL
D004797:Enzyme-Linked Immunosorbent Assay; D005427:Flocculation; D011237:Predictive Value of Tests; D013744:Tetanus Toxin / Q000032:analysis*
D013744:Tetanus Toxin