The organisation of the mouse chromogranin B (secretogranin I) gene.
A cosmid clone containing the gene for mouse chromogranin B (secretogranin I), a secretory protein found in secretory granules of most endocrine cells and neurons, was isolated and sequenced. The chromogranin B protein was found to be encoded by 5 exons which correspond to the cleaved signal peptide, the short N-terminal sequence preceding the disulfide-bonded loop structure, the disulfide-bonded loop structure itself, the large, variable region comprising approximately 90% of the protein, and the conserved C-terminal sequence. The promoter region of the chromogranin B gene is very GC-rich and contains a CATAA motif, a cAMP-responsive element and an Sp1 binding site.
Pohl
T M
TM
Differentiation Programmes, European Molecular Biology Laboratory, Heidelberg, FRG.
Phillips
E
E
Song
K Y
KY
Gerdes
H H
HH
Huttner
W B
WB
Rüther
U
U
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D053378:Chromogranin B; D002864:Chromogranins / Q000235:genetics*; D003001:Cloning, Molecular; D005091:Exons; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics; D051381:Rats; D015183:Restriction Mapping; D013045:Species Specificity
C506134:CHGB protein, human; D053378:Chromogranin B; D002864:Chromogranins; D009419:Nerve Tissue Proteins; D011506:Proteins; C506137:chromogranin B, mouse; C506140:chromogranin B, rat