title: Visible-light system for detecting doxorubicin contamination on skin and surfaces.
abstract: A portable system that uses fluorescence stimulated by visible light to identify doxorubicin contamination on skin and surfaces was studied. When activated by violet-blue light in the 465-nm range, doxorubicin fluoresces, emitting orange-red light in the 580-nm range. The light source to stimulate fluorescence was a slide projector with a filter to selectively pass short-wave (blue) visible light. Fluorescence was both observed visually with viewing spectacles and photographed. Solutions of doxorubicin in sterile 0.9% sodium chloride injection were prepared in nine standard concentrations ranging from 2 to 0.001 mg/mL. Droplets of each admixture were placed on stainless steel, laboratory coat cloth, pieces of latex examination glove, bench-top absorbent padding, and other materials on which antineoplastics might spill or leak. These materials then were stored for up to eight weeks and photographed weekly. The relative ability of water, household bleach, hydrogen peroxide solution, and soap solution to deactivate doxorubicin was also measured. Finally, this system was used to inspect the antineoplastic-drug preparation and administration areas of three outpatient cancer clinics for doxorubicin contamination. Doxorubicin fluorescence was easily detectable with viewing spectacles when a slide projector was used as the light source. The photographic method was sensitive for doxorubicin concentrations from 2.0 to 0.001 mg/mL. Immersion of study materials in bleach for one minute eliminated detectable fluorescence. Doxorubicin contamination is detectable for at least eight weeks in the ambient environment. Probable doxorubicin contamination was detected in two of the three clinics surveyed. A safe, portable system that uses fluorescence stimulated by visible light is a sensitive method for detecting doxorubicin on skin and surfaces.
authors.affiliation: Department of Environmental Health, University of Cincinnati, OH 45267-0056.
authors.forename: J
authors.initials: J
authors.lastname: Van Raalte
authors.forename: C
authors.initials: C
authors.lastname: Rice
authors.forename: C E
authors.initials: CE
authors.lastname: Moss
mesh_terms: D004317:Doxorubicin / Q000032:analysis* / Q000528:radiation effects; D005453:Fluorescence; D006801:Humans; D008027:Light; D010783:Photometry; D012867:Skin / Q000032:analysis*; D014466:Ultraviolet Rays
chemical_list: D004317:Doxorubicin