[Gene 26 of bacteriophage T4 basal plate. I. Two expression products of the cloned gene].
We have cloned the 1.9 kb EcoRV-BglII DNA fragment with T4 genes 51, 26, and 25 into the expression plasmid pT7-5 carrying a T7 promoter. The resulting recombinant plasmid, pRR5-3, contained T4 genes 26 and 25 in the correct orientation for expression. We expressed these genes using the T7 RNA polymerase/promoter system and the synthesis of three polypeptides with the molecular masses of approximately 24, 15, and 8-9 kDa was observed. Expression of genes from the subcloned DNA fragments and from the fragments carrying deletions was studied as well and the 15 kDa protein appeared to be the product of gene 25, while 24 kDa and 8-9 kDa proteins were identified as products of gene 26. The 8-9 kDa protein was shown to be expressed from the end region of gene 26. Having analysed the proteins expressed from the fragments carrying fusion of genes 26 and 25 we supposed two products of gene 26 to be encoded by the same open reading frame.
Vaĭshkunaĭte
R I
RI
Nivinskas
R G
RG
D003001:Cloning, Molecular; D004587:Electrophoresis, Agar Gel; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D010957:Plasmids; D011995:Recombination, Genetic; D015183:Restriction Mapping; D013604:T-Phages / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics* / Q000302:isolation & purification
D014764:Viral Proteins
Gen 26 bazal'noĭ plastinki bakteriofaga T4. I. Dva produkta ékspressii klonirovannogo gena.