title: Crystal structure of plakalbumin, a proteolytically nicked form of ovalbumin. Its relationship to the structure of cleaved alpha-1-proteinase inhibitor.
abstract: The crystal structure of plakalbumin, a proteolytically nicked form of ovalbumin, has been determined to a resolution of 2.8 A by the isomorphous replacement method and preliminary refinement. The structure closely resembles that of the cleaved form of alpha-1-proteinase inhibitor, with some important exceptions. The disposition of the new carboxyl chain terminus liberated by proteolysis is different with respect to the central beta-sheet A in the structures of these two molecules. In alpha-1-proteinase inhibitor, the new chain terminus inserts in beta-sheet A to add a middle strand to the sheet. In plakalbumin, this strand remains free near the site at which the cleavage occurs. A structural basis for this difference in behavior is proposed from the structures and sequences of these two molecules and other members of the serpin family. The structures and positions of the putative signal peptide of ovalbumin, the several post-translational modifications, and the relationship of the intron-exon patterns of plakalbumin and alpha-1-proteinase inhibitor to their protein structures are also described.
authors.affiliation: Department of Biochemistry and Molecular Biophysics, Virginia Commonwealth University, Richmond 23298.
authors.forename: H T
authors.initials: HT
authors.lastname: Wright
authors.forename: H X
authors.initials: HX
authors.lastname: Qian
authors.forename: R
authors.initials: R
authors.lastname: Huber
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D004583:Electrons; D008954:Models, Biological; D008969:Molecular Sequence Data; D010047:Ovalbumin / Q000032:analysis* / Q000235:genetics; D010446:Peptide Fragments / Q000032:analysis*; D010449:Peptide Mapping; D011487:Protein Conformation; D011499:Protein Processing, Post-Translational; D012984:Software; D013237:Stereoisomerism; D014961:X-Ray Diffraction; D000515:alpha 1-Antitrypsin / Q000235:genetics
chemical_list: D010446:Peptide Fragments; D000515:alpha 1-Antitrypsin; C038141:plakalbumin; D010047:Ovalbumin