title: Genetic and physiological characterization of the purine salvage pathway in the archaebacterium Methanobacterium thermoautotrophicum Marburg.
abstract: The enzymes involved in the purine interconversion pathway of wild-type and purine analog-resistant strains of Methanobacterium thermoautotrophicum Marburg were assayed by radiometric and spectrophotometric methods. Wild-type cells incorporated labeled adenine, guanine, and hypoxanthine, whereas mutant strains varied in their ability to incorporate these bases. Adenine, guanine, hypoxanthine, and xanthine were activated by phosphoribosyltransferase activities present in wild-type cell extracts. Some mutant strains simultaneously lost the ability to convert both guanine and hypoxanthine to the respective nucleotide, suggesting that the same enzyme activates both bases. Adenosine, guanosine, and inosine phosphorylase activities were detected for the conversion of base to nucleoside. Adenine deaminase activity was detected at low levels. Guanine deaminase activity was not detected. Nucleoside kinase activities for the conversion of adenosine, guanosine, and inosine to the respective nucleotides were detected by a new assay. The nucleotide-interconverting enzymes AMP deaminase, succinyl-AMP synthetase, succinyl-AMP lyase, IMP dehydrogenase, and GMP synthetase were present in extracts; GMP reductase was not detected. The results indicate that this autotrophic methanogen has a complex system for the utilization of exogenous purines.
authors.affiliation: Department of Botany and Microbiology, University of Oklahoma, Norman 73019.
authors.forename: V E
authors.initials: VE
authors.lastname: Worrell
authors.forename: D P
authors.initials: DP
authors.lastname: Nagle
mesh_terms: D019605:Euryarchaeota / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D007041:Hypoxanthine Phosphoribosyltransferase / Q000032:analysis; D009154:Mutation; D010430:Pentosyltransferases / Q000032:analysis; D011687:Purines / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D011687:Purines; D010430:Pentosyltransferases; C022484:purine phosphoribosyltransferase; D007041:Hypoxanthine Phosphoribosyltransferase