Cloning, characterisation and expression of vitellogenin gene of Oreochromis aureus (Teleostei, Cichlidae).
cDNA synthesised from liver Poly A+ RNA of estradiol-stimulated male Oreochromis aureus was ligated to PTZ-19R and transformed into E. coli. TG-1 to yield 6 x 10(4) clones/ug DNA, of which 6% was vitellogenin positive. Restriction maps indicate 4 possible subgroups of vitellogenin cDNA, with homology, observed by Southern hybridisation, at a fragment flanked by Pst 1 sites. When the amino acid sequence derived from the DNA sequence of pOA Vg 62 was aligned with that of S. gairdneri, X. laevis, C. elegans and D. melanogaster, homologies of 63%, 22%, 21% and 15%, respectively, were obtained. Estradiol induced a vitellogenin gene transcript of 6500 nucleotides at 6 h and reached a maximum at 72 h after stimulation.
Ding
J L
JL
Department of Zoology, National University of Singapore, Kent Ridge.
Ho
B
B
Valotaire
Y
Y
LeGuellec
K
K
Lim
E H
EH
Tay
S P
SP
Lam
T J
TJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004958:Estradiol / Q000494:pharmacology; D005786:Gene Expression Regulation; D008297:Male; D008969:Molecular Sequence Data; D010471:Perches / Q000235:genetics*; D010473:Perciformes / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014170:Transformation, Genetic; D014819:Vitellogenins / Q000235:genetics*
D014819:Vitellogenins; D004958:Estradiol; D004247:DNA