Characterization of rat brain NCAM mRNA using DNA oligonucleotide probes.
A number of different isoforms of the neural cell adhesion molecule (NCAM) have been identified. The difference between these is due to alternative splicing of a single NCAM gene. In rat brain NCAM mRNAs with sizes of 7.4, 6.7, 5.2, 4.3 and 2.9 kb have been reported. We have synthesized six DNA oligonucleotides, that hybridize to different exons in the NCAM gene. Furthermore we have constructed three oligonucleotides, that exclusively hybridize to mRNAs lacking certain exons, by letting them consist of sequences adjacent to both sides of the splice sites. By means of these probes we have characterized the five NCAM mRNAs in rat brain.
Andersson
A M
AM
Research Center of Medical Biotechnology, University of Copenhagen, Denmark.
Gaardsvoll
H
H
Giladi
E
E
Dahl
B
B
Bock
E
E
D000818:Animals; D015152:Blotting, Northern; D001923:Brain Chemistry; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics*; D015342:DNA Probes; D005091:Exons; D008099:Liver / Q000032:analysis; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats
D015816:Cell Adhesion Molecules, Neuronal; D015342:DNA Probes; D015345:Oligonucleotide Probes; D012333:RNA, Messenger