title: The respective 27 kDa and 28 kDa protein kinase C substrates in vascular endothelial and MCF-7 cells are most probably heat shock proteins.
abstract: The phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) stimulated the phosphorylation of two distinct 27 kDa and 28 kDa proteins, respectively, in bovine vascular endothelial cells and in MCF-7 human breast cancer cells. These protein phosphorylation events were correlated to striking opposite cell growth responses to TPA, i.e., stimulation of vascular endothelial cell proliferation and inhibition of MCF-7 cell growth. Exposure of both vascular endothelial and MCF-7 cells to heat shock induced synthesis of the respective 27 kDa and 28 kDa proteins among a set of common and distinct other proteins as well as an increase in the degree of phosphorylation of the two 27 kDa and 28 kDa proteins. These results suggest that the two protein kinase C substrates very likely belong to the family of low molecular mass stress proteins.
authors.affiliation: Inserm U168, Department of Endocrinology, CHU Rangueil, Université Paul Sabatier, Toulouse, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Darbon
authors.forename: M
authors.initials: M
authors.lastname: Issandou
authors.forename: J F
authors.initials: JF
authors.lastname: Tournier
authors.forename: F
authors.initials: F
authors.lastname: Bayard
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000201:enzymology*; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D008970:Molecular Weight; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism*; D013379:Substrate Specificity; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D006360:Heat-Shock Proteins; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate