Removal of apoprotein-B-containing lipoproteins by plasmapheresis using immobilized phosphorylcholine-binding protein affinity adsorbent.
Rat serum phosphorylcholine binding protein was earlier shown to bind lipoproteins containing apoproteins B and E from human very low and low density lipoproteins. The present studies were undertaken to show the effectiveness of rat serum phosphorylcholine-binding protein immobilized on Sepharose affinity column to remove apoprotein-B-containing lipoproteins from normal and hypercholesterolemic rabbit plasma, when used in a plasmapheresis system. The maximum in vitro binding of very low and low density lipoproteins from hypercholesterolemic rabbit plasma to the affinity adsorbent was Ca2+ dependent, and the cholesterol bound to the column at the optimum calcium concentration (2.5 mM) was 21% of the total plasma cholesterol applied. The in vivo binding of total cholesterol from normal and hypercholesterolemic rabbit plasma during plasmapheresis ranged from 0.22 to 7.7%. Total mass of cholesterol bound ranged from 3.86 and 27.52 mg at plasma cholesterol concentrations 13.8 and 282 mg/dL, respectively. Most (greater than 95%) of the bound cholesterol was associated with very low and low density lipoproteins. These studies show the ability of immobilized rat serum phosphorylcholine-binding protein to lower the atherogenic apoprotein-B-containing lipoproteins from plasma of hypercholesterolemic rabbits.
Saxena
U
U
Department of Biochemistry, Memorial University, St. John's, Nfld., Canada.
Francis-Collins
J
J
Hall
J
J
Legal
Y
Y
Barrowman
J
J
Nagpurkar
A
A
Mookerjea
S
S
D000327:Adsorption; D000818:Animals; D001055:Apolipoproteins B / Q000097:blood / Q000302:isolation & purification*; D002118:Calcium / Q000494:pharmacology; D002352:Carrier Proteins / Q000378:metabolism; D006937:Hypercholesterolemia / Q000097:blood / Q000628:therapy; D008297:Male; D010767:Phosphorylcholine; D010956:Plasmapheresis / Q000379:methods*; D011817:Rabbits; D051381:Rats; D012685:Sepharose
D001055:Apolipoproteins B; D002352:Carrier Proteins; C030929:Crp protein, rat; D010767:Phosphorylcholine; D012685:Sepharose; D002118:Calcium