Experimental considerations on the measurement of prostaglandins during long-time incubations of neonatal mouse calvaria.
The influence of experimental conditions during long-time (72 h) incubations of neonatal mouse calvaria on the measurement of prostaglandins was investigated. Incubations of the cultured calvaria were carried out in the presence and absence of stimulating agents of bone resorption, such as thrombin and parathyroid hormone. It was found that during the first 24 h prostaglandin levels, estimated by gas chromatography negative ion chemical ionization mass spectrometry, did not correlate with calcium liberation, but were merely an artefact resulting from surgery by preparing the calvaria.
Leis
H J
HJ
University Children's Hospital, Dept. of Mass Spectrometry, University of Graz, Austria.
Malle
E
E
Hoffmann
O
O
Klaushofer
K
K
Fratzl-Zelman
N
N
Koller
K
K
Peterlik
M
M
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis; D000818:Animals; D000831:Animals, Newborn; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism*; D015232:Dinoprostone / Q000096:biosynthesis; D007700:Kinetics; D051379:Mice; D008815:Mice, Inbred Strains; D009924:Organ Culture Techniques; D010281:Parathyroid Hormone / Q000494:pharmacology; D011453:Prostaglandins / Q000378:metabolism*; D013917:Thrombin / Q000494:pharmacology
D010281:Parathyroid Hormone; D011453:Prostaglandins; D015121:6-Ketoprostaglandin F1 alpha; D013917:Thrombin; D015232:Dinoprostone