Translocation of chromosomes 16 and 18 in oxygen radical-transformed human lung fibroblasts.
Human lung fibroblasts (MRC5) were treated chronically with the oxygen radical-generating system hypoxanthine plus xanthine oxidase and assayed for malignant transformation in the soft agar colony assay. After a thrice weekly exposure to oxygen radicals for 4 and 5 weeks, there was a significant number of transformants compared to controls. In 4 separate experiments, karyotypes of the malignant transformants were examined. 22/75 metaphases exhibited karyotypic abnormalities and in 13/22 of the abnormal karyotypes, a t16:18 (p13.3,q21) translocation was observed. This genetic lesion may represent a marker for oxygen radical-induced malignant transformation in mammalian cells.
Weitberg
A B
AB
Division of Hematology/Oncology, Roger Williams Cancer Center, Providence, Rhode Island.
Corvese
D
D
D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced / Q000235:genetics*; D002885:Chromosomes, Human, Pair 16; D002887:Chromosomes, Human, Pair 18; D005347:Fibroblasts; D005609:Free Radicals; D005819:Genetic Markers; D006801:Humans; D007621:Karyotyping; D008168:Lung; D010100:Oxygen / Q000633:toxicity*; D014178:Translocation, Genetic
D005609:Free Radicals; D005819:Genetic Markers; D010100:Oxygen