Basigin, a new, broadly distributed member of the immunoglobulin superfamily, has strong homology with both the immunoglobulin V domain and the beta-chain of major histocompatibility complex class II antigen.
Lotus tetragonolobus agglutinin (LTA) binds preferentially to early embryonic cells in the mouse. The affinity-purified antibody raised against LTA receptors from embryonal carcinoma cells were used to screen a lambda gt11 expression library of F9 embryonal carcinoma cells, resulting in detection of a cDNA clone specifying a new glycoprotein termed "basigin." The glycoprotein has been suggested to be a transmembrane one, and was found to be a new member of the immunoglobulin (Ig) superfamily. The molecular weight of basigin was largely in the range between 43,000 and 66,000, while that of the peptide portion with a putative signal sequence was inferred to be about 30,000. Significant levels of basigin mRNA were detected not only in embryonal carcinoma cells, but also in mouse embryos at 9-15 days of gestation and in various organs of the adult mouse. The Ig-like domain of basigin is unique, since it has strong homology to both the beta-chain of major histocompatibility class II antigen and the Ig V domain. The number of amino acids between the two conserved cysteine residues is intermediate between those of the Ig V and C domains. Therefore, basigin is an interesting protein in connection with the molecular evolution of the superfamily.
Miyauchi
T
T
Japan Immunoresearch Laboratories, Gumma.
Kanekura
T
T
Yamaoka
A
A
Ozawa
M
M
Miyazawa
S
S
Muramatsu
T
T
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D030161:Avian Proteins; D001483:Base Sequence; D051926:Basigin; D001665:Binding Sites; D001798:Blood Proteins; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005260:Female; D015870:Gene Expression; D005803:Genes, Immunoglobulin / Q000235:genetics*; D006023:Glycoproteins / Q000235:genetics*; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D037102:Lectins / Q000235:genetics; D008562:Membrane Glycoproteins; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D011247:Pregnancy; D011817:Rabbits; D012689:Sequence Homology, Nucleic Acid; D013724:Teratoma / Q000473:pathology; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D030161:Avian Proteins; D001798:Blood Proteins; C102624:Bsg protein, Gallus gallus; C497887:Bsg protein, mouse; C079432:Bsg protein, rat; D006023:Glycoproteins; D000949:Histocompatibility Antigens Class II; D007135:Immunoglobulin Variable Region; D037102:Lectins; D008562:Membrane Glycoproteins; C027112:fucose-binding lectin; D051926:Basigin; D004247:DNA