title: [Synthesis of N-glycoproteins with a native type of carbohydrate-peptide bond].
abstract: Reaction of beta-oligoglycosylamines obtained from carbohydrate chains of N-glycoproteins (ovalbumin, ovomucoid, riboflavin-binding glycoprotein from hen egg white, and asialofetuin) with bovine serum albumin, lysozyme, and poly(L-Asp) in presence of water-soluble carbodiimide gave rise to a series of glycoconjugates, modelling natural N-glycoproteins. Carbohydrate-peptide bond was shown to be of N-glycosylamide type with participation of Asp and Glu residues. The method allows one to obtain synthetic N-glycoproteins from oligomannoside, complex and hybrid oligosaccharide chains, and may find application both in biochemistry and biotechnology for improvement of physico-chemical properties of unglycosylated proteins.
authors.forename: L M
authors.initials: LM
authors.lastname: Likhosherstov
authors.forename: V E
authors.initials: VE
authors.lastname: Piskarev
authors.forename: V A
authors.initials: VA
authors.lastname: Derevitskaia
authors.forename: N K
authors.initials: NK
authors.lastname: Kochetkov
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D006023:Glycoproteins / Q000138:chemical synthesis*; D009844:Oligosaccharides; D010455:Peptides
chemical_list: D006023:Glycoproteins; D009844:Oligosaccharides; D010455:Peptides
vernacular_title: Sintez N-glikoproteinov s prirodnym tipom uglevod-peptidnoĭ sviazi.