Synthesis of peptide-immunogens corresponding to amino acid sequences from human histocompatibility class II membrane glycoproteins.
Six peptides with amino acid sequences of human histocompatibility Class II membrane glycoproteins were synthesized by conventional solution methods. Five peptides were prepared by stepwise procedures from the carboxyterminus. The sixth was synthesized by fragment condensation (5 + 10 coupling). Antibodies to synthetic peptides were then used to locate exposed and buried regions in the membrane glycoproteins.
Chillemi
F
F
Department of Organic and Industrial Chemistry, University of Milan, Italy.
Cappelletti
S
S
Francescato
P
P
Chersi
A
A
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004024:Dicyclohexylcarbodiimide; D006023:Glycoproteins / Q000276:immunology; D000949:Histocompatibility Antigens Class II; D007106:Immune Sera / Q000276:immunology; D007114:Immunization; D008969:Molecular Sequence Data; D010047:Ovalbumin / Q000276:immunology; D010455:Peptides / Q000138:chemical synthesis* / Q000276:immunology; D011817:Rabbits; D014230:Triazoles
D000941:Antigens; D006023:Glycoproteins; D000949:Histocompatibility Antigens Class II; D007106:Immune Sera; D010455:Peptides; D014230:Triazoles; D004024:Dicyclohexylcarbodiimide; D010047:Ovalbumin; C011852:1-hydroxybenzotriazole