Isolation of a highly enriched plasma membrane fraction of Trypanosoma brucei by free-flow electrophoresis.
A procedure is described whereby a highly enriched plasma membrane fraction was isolated from Trypanosoma brucei by the technique of preparative free-flow electrophoresis. The purity of the plasma membrane fraction was monitored by electron microscopy and by marker enzymology, and is compared to those obtained by previous methods. Proteins associated with plasma membrane fraction were analyzed by SDS-PAGE and phase separated in Triton X-114.
Siddiqui
A A
AA
Department of Zoology, University of Western Ontario, London, Canada.
Zhou
Y
Y
Podesta
R B
RB
Clarke
M W
MW
D000818:Animals; D002458:Cell Fractionation; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure*; D004586:Electrophoresis; D008854:Microscopy, Electron; D014346:Trypanosoma brucei brucei / Q000648:ultrastructure*