title: Human bone marrow processing using Cobe 2991 and CS 3000 blood cell separators for further ex vivo manipulation.
abstract: Several automated procedures are now available to enrich stem cells from large bone marrow (BM) volumes prior to ex vivo treatment or cryopreservation. This report details our experience using a ficoll-hypaque (F/H) gradient on Cobe 2991 cell washer and a CS 3000 continuous flow separator, on 90 BM processed for allogeneic and autologous transplantation. In the Cobe series, from 70 BM aspirates, 89 +/- 5% of the original mononuclear cells (MNC) was found in the light density fraction with a erythrocytes (RBC) and granulocytes (PMN) removal of 98 +/- 1 and 97 +/- 4.5%, respectively. Over 80% of the initial myeloid precursors (CFU-GM) were recovered in a small final volume. Twenty BM processing were performed with a CS 3000 separator using program "3" and granulo chamber. This technique yielded 86 +/- 9% of the initial MNC while 85 +/- 10% of RBC and 90 +/- 1.1% of PMN was removed. Over 75% of the original CFU-GM was recovered in the final product. Both techniques are effective to large-scale purification of progenitor cells and readily available as routine procedures for marrow processing.
authors.affiliation: Divisione di Ematologia e Centro Trasfusionale, Ospeda-le Civile, Pescara, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Angelini
authors.forename: A
authors.initials: A
authors.lastname: Dragani
authors.forename: A
authors.initials: A
authors.lastname: Iacone
authors.forename: D
authors.initials: D
authors.lastname: D'Antonio
authors.forename: P
authors.initials: P
authors.lastname: Accorsi
authors.forename: A M
authors.initials: AM
authors.lastname: Quaglietta
authors.forename: A
authors.initials: A
authors.lastname: Berardi
authors.forename: G
authors.initials: G
authors.lastname: Fioritoni
authors.forename: P
authors.initials: P
authors.lastname: Di Bartolomeo
authors.forename: G
authors.initials: G
authors.lastname: D'Emilio
mesh_terms: D000293:Adolescent; D000328:Adult; D001773:Blood Cells / Q000166:cytology*; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D006801:Humans; D008875:Middle Aged; D013048:Specimen Handling / Q000379:methods