Factors affecting the induction of 11 alpha-hydroxylase of progesterone in the filamentous fungus Rhizopus nigricans.
The 11 alpha-hydroxylase of progesterone was induced in the filamentous fungus Rhizopus nigricans ATCC 6227b with different steroids as inducers and the induction process was optimized in regard to the age of the mycelium, to the concentration of the inducer and to the time of induction. Deoxycorticosterone and testosterone, steroids with higher polarity of the side-chain than progesterone, although poorer substrates for in vivo hydroxylation than progesterone, induced more enzyme compared to progesterone. Other alterations in the steroidal ring system examined diminished the induction capability of the inducing steroid to different extent. The highest 11 alpha-hydroxylating activity, if expressed on the basis of mycelial wet weight, was achieved with 18 h old mycelium which was induced for 2 h with 0.30 mM deoxycorticosterone.
Hudnik-Plevnik
T
T
Institute of Biochemistry, Medical Faculty, University of Ljubljana, Yugoslavia.
Cresnar
B
B
D003577:Cytochrome P-450 Enzyme System; D004790:Enzyme Induction / Q000187:drug effects; D012739:Gonadal Steroid Hormones / Q000494:pharmacology*; D011278:Pregnanes / Q000494:pharmacology*; D012233:Rhizopus / Q000187:drug effects / Q000201:enzymology*; D013045:Species Specificity; D013250:Steroid Hydroxylases / Q000096:biosynthesis*; D013997:Time Factors
D012739:Gonadal Steroid Hormones; D011278:Pregnanes; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; C074514:steroid 11-alpha-hydroxylase