[A comparison of methods for the determination of A2 hemoglobin].
Three different methods to assess haemoglobin A2 concentration (microchromatography and cellulose acetate electrophoresis with further elution of the bands or measured by densitometry) were compared among them. A statistical significant difference (P less than 0.05) was found between densitometry and the other two methods. Microchromatography and the elution method showed no statistical difference between them. The most important technical aspects of each method as well as some possible error factors were also discussed.
Mora
L A
LA
Laboratorio de Investigación, Hospital Nacional de Niños Dr. Carlos Sáenz Herrera, C.C.S.S. San José, Costa Rica.
Ramírez
M E
ME
Jiménez
R
R
D002675:Child, Preschool; D002848:Chromatography, DEAE-Cellulose / Q000379:methods*; D003720:Densitometry; D004586:Electrophoresis / Q000379:methods*; D004588:Electrophoresis, Cellulose Acetate / Q000379:methods*; D005260:Female; D006441:Hemoglobin A / Q000032:analysis*; D006443:Hemoglobin A2 / Q000032:analysis*; D006801:Humans; D007223:Infant; D008297:Male
D006441:Hemoglobin A; D006443:Hemoglobin A2
Comparación de métodos para la cuantificación de hemoglobina A2.