[Effects of intracellular Na+/K+ ratio on histone gene expression in ascitic cells of leukemia P-388 and Ehrlich cancer].
The intracellular Na+/K+ ratio was studied for its effect on histone genes expression in Ehrlich carcinoma and leukemia P-388 ascites cells. After equilibration with certain Na+/K+ ratio buffers at 0-4 degrees C the cells were treated during 1 h at 37 degrees C with ouabain added to the corresponding medium. Then total RNAs were extracted and analyzed by the reaction of blot-hybridization with histone genes cluster in plasmid p604. The maximal level of histone genes expression in leukemia P-388 cells was observed under conditions when intracellular Na+/K+ ratio corresponded to those at S-phase of the mitotic cycle. In Ehrlich carcinoma ascites cells the expression of histone genes was not dependent on the Na+/K+ ratio. A conclusion is drawn that the intracellular ratio of monovalent cations is one of the possible mechanisms which regulates the gene expression during the mitotic cycle.
Smirnova
N V
NV
Kolosov
E V
EV
Kaz'min
S D
SD
D000818:Animals; D001345:Autoradiography; D002286:Carcinoma, Ehrlich Tumor / Q000235:genetics / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005260:Female; D015973:Gene Expression Regulation, Leukemic; D015972:Gene Expression Regulation, Neoplastic; D006657:Histones / Q000235:genetics*; D006706:Homeostasis; D066298:In Vitro Techniques; D007941:Leukemia P388 / Q000235:genetics / Q000378:metabolism*; D007942:Leukemia, Experimental / Q000378:metabolism*; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D008938:Mitosis; D011188:Potassium / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*
D004273:DNA, Neoplasm; D006657:Histones; D012964:Sodium; D011188:Potassium
Vliianie vnutrikletochnogo sootnosheniia Na+/K+ na ékspressiiu gistonovykh genov v astsitnykh kletkakh leĭkoza P-388 i raka Erlikha.