[The heterogeneity of sorbitol dehydrogenase from the cytoplasm of mammalian brain cells].
The multiple molecular forms of sorbitoldehydrogenase in cytoplasm of brain cells of bull, ground squirrel, guinea-pig, rat, hamster and mouse have been found using the method of electrophoresis in polyacrylamide gel and the subsequent specific dyeing for the fermentative activity. All revealed zones of activity are related to the slowly migrating ones. A set of multiple molecular forms from different sources is various. A form with relative electrophoretic activity 0.385 is found in all analyzed animals. The conditions for obtaining of distinct zones of activity on zymograms are chosen.
Sudovtsov
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Aslanidi
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D000818:Animals; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D006224:Cricetinae; D003593:Cytoplasm / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006168:Guinea Pigs; D007527:Isoenzymes / Q000032:analysis*; D007064:L-Iditol 2-Dehydrogenase / Q000032:analysis*; D008297:Male; D051379:Mice; D051381:Rats; D011919:Rats, Inbred Strains; D012589:Sciuridae; D013045:Species Specificity; D013401:Sugar Alcohol Dehydrogenases / Q000032:analysis*
D007527:Isoenzymes; D013401:Sugar Alcohol Dehydrogenases; D007064:L-Iditol 2-Dehydrogenase
Geterogennost' sorbitoldegidrogenazy iz tsitoplazmy kletok mozga mlekopitaiushchikh.