title: Electric field mediated transfer of enzymes into human oocytes.
abstract: In an attempt to develop a simple, atraumatic technique of introducing macromolecules and enzymes into human oocytes, we have investigated the use of electric field mediated transfer. Eighty-seven percent of human oocytes showed cytochemical evidence for the transfer of the enzyme horseradish peroxidase (HRP). The cell viability after this procedure was 89% as determined by the vital dye rhodamine-123. This study demonstrates that the enzyme HRP can be transferred into human oocytes using the electric field mediated method. Functional activity of the enzyme was maintained after this transfer.
authors.affiliation: Ohio State University College of Medicine, Columbus.
authors.forename: J W
authors.initials: JW
authors.lastname: Douglas
authors.forename: M H
authors.initials: MH
authors.lastname: Kim
authors.forename: B E
authors.initials: BE
authors.lastname: Batten
mesh_terms: D015100:3,3'-Diaminobenzidine / Q000008:administration & dosage; D004560:Electricity; D004798:Enzymes / Q000008:administration & dosage*; D005260:Female; D006735:Horseradish Peroxidase / Q000008:administration & dosage; D006801:Humans; D006861:Hydrogen Peroxide / Q000008:administration & dosage; D066298:In Vitro Techniques; D009865:Oocytes / Q000201:enzymology*
chemical_list: D004798:Enzymes; D015100:3,3'-Diaminobenzidine; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase