title: Transcription of the procyclic acidic repetitive protein genes of Trypanosoma brucei.
abstract: The procyclic acidic repetitive protein (parp) genes of Trypanosoma brucei encode a small family of abundant surface proteins whose expression is restricted to the procyclic form of the parasite. They are found at two unlinked loci, parpA and parpB; transcription of both loci is developmentally regulated. The region of homology upstream of the A and B parp genes is only 640 base pairs long and may contain sequences responsible for transcriptional initiation and regulation. Transcription upstream of this putative promoter region is not developmentally regulated and is much less active than that of the parp genes; the polymerase responsible is inhibited by alpha-amanitin, whereas that transcribing the parp genes is not. Transcription of the parp genes is strongly stimulated by low levels of UV irradiation. The putative parp promoter, when placed upstream of the chloramphenicol acetyltransferase gene, is sufficient to cause production of chloramphenicol acetyltransferase in a T. brucei DNA transformation assay. Taken together, these results suggest that a promoter for an alpha-amanitin-resistant RNA polymerase lies less than 600 nucleotides upstream of the parp genes.
authors.affiliation: Rockefeller University, New York, New York 10021-6399.
authors.forename: C E
authors.initials: CE
authors.lastname: Clayton
authors.forename: J P
authors.initials: JP
authors.lastname: Fueri
authors.forename: J E
authors.initials: JE
authors.lastname: Itzhaki
authors.forename: V
authors.initials: V
authors.lastname: Bellofatto
authors.forename: D R
authors.initials: DR
authors.lastname: Sherman
authors.forename: G S
authors.initials: GS
authors.lastname: Wisdom
authors.forename: S
authors.initials: S
authors.lastname: Vijayasarathy
authors.forename: M R
authors.initials: MR
authors.lastname: Mowatt
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D004307:Dose-Response Relationship, Radiation; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D015800:Protozoan Proteins; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic / Q000528:radiation effects; D014162:Transfection; D014346:Trypanosoma brucei brucei / Q000235:genetics*; D014466:Ultraviolet Rays; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000235:genetics*
chemical_list: D000953:Antigens, Protozoan; D008562:Membrane Glycoproteins; D015800:Protozoan Proteins; D014643:Variant Surface Glycoproteins, Trypanosoma; C053277:procyclic acidic repetitive protein, Trypanosoma