title: Expression of mRNAs for pore-forming protein and two serine esterases in murine primary and cloned effector lymphocytes.
abstract: The cDNAs encoding several proteins present in the granules of cytolytic effector lymphocytes have now been cloned. These include the cytolytic pore-forming protein (PFP) or perforin, and at least six serine esterases (SE), also called granzymes. The cDNA probes for PFP, SE-1, and SE-2 are used here to study the expression of these proteins in murine primary effector lymphocytes. Among the stimuli effective in inducing the expression of PFP, SE-1, and SE-2 were recombinant interleukin-2, the lectin concanavalin A in the presence of phorbol esters, and allogeneic cells in mixed lymphocyte cultures. Some correlation was seen between the levels of PFP and SE mRNAs and cytotoxicity measured in a standard 51Cr release assay. We also examined a panel of 13 cloned cytotoxic T lymphocyte (CTL) lines and found that mRNAs for PFP and SE-2 were expressed in all CTL lines, including some that were previously considered not to produce PFP. Twelve of the 13 CTL lines also proved to possess the mRNA for SE-1. One thymoma cell line, TIMI.4, did not express mRNA for PFP, although it expressed mRNA for SE-1 and SE-2.
authors.affiliation: Laboratory of Cellular Physiology and Immunology, Rockefeller University, New York, New York 10021.
authors.forename: S V
authors.initials: SV
authors.lastname: Joag
authors.forename: C C
authors.initials: CC
authors.lastname: Liu
authors.forename: B S
authors.initials: BS
authors.lastname: Kwon
authors.forename: W R
authors.initials: WR
authors.lastname: Clark
authors.forename: J D
authors.initials: JD
authors.lastname: Young
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D002478:Cells, Cultured; D002999:Clone Cells; D004950:Esterases / Q000235:genetics*; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D012333:RNA, Messenger / Q000096:biosynthesis*; D013602:T-Lymphocytes, Cytotoxic / Q000378:metabolism*
chemical_list: D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D052899:Pore Forming Cytotoxic Proteins; D012333:RNA, Messenger; D054353:Perforin; D004950:Esterases; C022635:serine esterase