A new approach to determine the stereospecificity in lipase catalysed hydrolysis using circular dichroism (CD): lipases produce optically active diglycerides from achiral triglycerides.
We describe a sensitive CD method for determining the stereospecificity in lipase (E.C.3.1.1.3) catalysed hydrolysis of triacyl glycerols into diacyl glycerols. The diglycerols were converted to chiral tert-butyldimethylsilylated 1,2- or 2,3-di-O-benzoyl-sn-glycerol (5 or 5'), and their CD was measured. This approach showed for the first time that lipases produce optically active diacyl glycerides from achiral tripalmitin and tribenzoyl glyceride with a variable extent of enantioselectivity depending on the acyl groups and the enzymes.
Uzawa
H
H
Department of Food Chemistry, Faculty of Agriculture, Tohoku University, Sendai, Japan.
Nishida
Y
Y
Ohrui
H
H
Meguro
H
H
D001592:Benzyl Alcohols; D002942:Circular Dichroism; D004075:Diglycerides / Q000378:metabolism*; D005989:Glycerides / Q000378:metabolism*; D006868:Hydrolysis; D008049:Lipase / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D014280:Triglycerides / Q000378:metabolism*
D001592:Benzyl Alcohols; D004075:Diglycerides; D005989:Glycerides; D014280:Triglycerides; D008049:Lipase