Optical quantification of caries-like lesions in vitro by use of a fluorescent dye.
An experimental method was developed for measurement of the fluorescence intensity of a dye that was introduced into caries-like lesions in vitro. A distinct pattern of change of fluorescence intensity with time appeared, displaying a plateau value and a peak value for each measurement. Both plateau and peak values showed a linear correlation with calcium loss, as measured with longitudinal microradiography. The correlation coefficients were r = 0.87 for plateau values and r = 0.89 for peak values. The difference in scattering by dry and wet caries lesions was also measured with the same equipment, which showed a linear correlation with calcium loss of r = -0.53.
Van de Rijke
J W
JW
Laboratory for Materia Technica, University of Groningen, The Netherlands.
Ten Bosch
J J
JJ
D000818:Animals; D002118:Calcium / Q000032:analysis; D002417:Cattle; D003731:Dental Caries / Q000473:pathology*; D003743:Dental Enamel / Q000032:analysis / Q000473:pathology*; D005336:Fiber Optic Technology; D005456:Fluorescent Dyes; D008852:Microradiography; D012542:Scattering, Radiation
D005456:Fluorescent Dyes; D002118:Calcium