title: Use of highly specific DNA probes and the polymerase chain reaction to detect Mycobacterium paratuberculosis in Johne's disease.
abstract: DNA probes that hybridize to a mycobacterial insertion sequence, IS900, present in multiple copies in the genome of Mycobacterium paratuberculosis were found to be highly specific for M. paratuberculosis. DNA sequences derived from IS900 were used to prepare DNA primers for detection and identification of M. paratuberculosis by the polymerase chain reaction. Highly specific direct detection of M. paratuberculosis DNA in feces from cattle with Johne's disease was obtained. The polymerase chain reaction test had a sensitivity equal to or greater than that obtained by standard culture techniques and was much more rapid, taking only hours compared with 6 to 12 weeks for culture.
authors.affiliation: Idexx Corporation, Portland, Maine 04101.
authors.forename: P H
authors.initials: PH
authors.lastname: Vary
authors.forename: P R
authors.initials: PR
authors.lastname: Andersen
authors.forename: E
authors.initials: E
authors.lastname: Green
authors.forename: J
authors.initials: J
authors.lastname: Hermon-Taylor
authors.forename: J J
authors.initials: JJ
authors.lastname: McFadden
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D005784:Gene Amplification; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000235:genetics* / Q000302:isolation & purification; D010283:Paratuberculosis / Q000175:diagnosis* / Q000382:microbiology; D016133:Polymerase Chain Reaction
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial