Purification of staphylococcal exfoliative toxin by high pressure liquid chromatography.
Exfoliative toxin (ET) isolated from a clinical strain of Staphylococcus aureus was purified to homogeneity, using a 3-step HPLC system. NH2-terminal 20 amino residues of purified ET was found to be identical with ETA of S. aureus TA (7), S. aureus TC16 (9) and S. aureus ZM (10), but stability of purified ET was completely different from that of ETA. This purification system gave a high yield of pure ET, which exhibited higher purity than specimens purified by more complicated and time-consuming procedures. It is useful for small-scale purification for the comparative study of ET and easy to scale up for preparative purification.
Sugai
M
M
Department of Microbiology, Hiroshima University, School of Dentistry, Japan.
Inoue
S
S
Hino
T
T
Kuwabara
M
M
Hong
Y M
YM
Miyake
Y
Y
Suginaka
H
H
D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D001427:Bacterial Toxins / Q000302:isolation & purification*; D001681:Biological Assay; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005083:Exfoliatins / Q000032:analysis / Q000302:isolation & purification* / Q000633:toxicity; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D013211:Staphylococcus aureus
D001427:Bacterial Toxins; D005083:Exfoliatins