Affinity of fibroblast growth factors for beta-cyclodextrin tetradecasulfate.
beta-Cyclodextrin tetradecasulfate was found to have a very strong affinity for fibroblast growth factor (FGF) and could substitute for heparin in FGF purification. Basic FGF was purified about 200,000-fold from a rat chondrosarcoma using a method of biaffinity chromatography in which the beta-cyclodextrin tetradecasulfate polymer was mixed with copper-Sepharose. This method takes advantage of the strong affinity of FGF for both beta-cyclodextrin tetradecasulfate and copper.
Yuen
S N
SN
Department of Surgery, Children's Hospital, Boston, Massachusetts.
Folkman
J
J
Weisz
P B
PB
Joullie
M M
MM
Ewing
W R
WR
D000818:Animals; D002478:Cells, Cultured; D002846:Chromatography, Affinity / Q000379:methods*; D003505:Cyclodextrins; D003912:Dextrins; D004591:Electrophoresis, Polyacrylamide Gel; D005346:Fibroblast Growth Factors / Q000302:isolation & purification*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D011485:Protein Binding; D051381:Rats; D011994:Recombinant Proteins; D013213:Starch; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D003912:Dextrins; D011994:Recombinant Proteins; D047392:beta-Cyclodextrins; C064089:beta-cyclodextrin tetradecasulfate; D005346:Fibroblast Growth Factors; D013213:Starch