title: Calcitonin gene-related peptide-positive neurons and fibers in the cat dorsal column nuclei.
abstract: An antiserum raised against the C-terminal region of rat alpha-calcitonin gene-related peptide has been used to investigate the morphology and topographical distribution of neurons and terminals containing calcitonin gene-related peptide in the cat dorsal column nuclear complex. Calcitonin gene-related peptide-positive fibers and axon terminals were denser in the cuneate nucleus than in the other dorsal column nuclei subdivisions and were observed throughout all rostrocaudal levels. They were densest in the dorsal and ventrolateral portions of the middle cuneate. Immunoreactive neurons were observed only in animals pre-treated with colchicine. In these cases, some calcitonin gene-related peptide-positive neurons were present in the cuneate and in the external cuneate. In double-labeling experiments, visualization of calcitonin gene-related peptide immunoreactivity in dorsal root ganglia neurons was combined with the retrograde transport of colloidal gold-labeled wheat germ agglutinin conjugated to inactive horseradish peroxidase injected in the cuneate nucleus. These experiments show that calcitonin gene-related peptide-positive fibers in the cuneate nucleus originate mostly from C3-C6 medium sized dorsal root ganglia neurons but also from some small and large neurons. These results suggest that calcitonin gene-related peptide-positive fibers may convey sensory information from a wide range of peripheral receptors.
authors.affiliation: Institute of Human Physiology, University of Ancona, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Fabri
authors.forename: F
authors.initials: F
authors.lastname: Conti
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D002415:Cats; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*
chemical_list: D015740:Calcitonin Gene-Related Peptide