[Antipeptide antibodies specific for the N-terminal site of lactate dehydrogenase from swine muscle].
Antipeptide antisera were obtained after immunization of rabbits with N-terminal peptide of pig muscle lactate dehydrogenase-5 containing the enzyme antigen determinant. The N-terminal peptide (1-32 amino acids) was conjugated with bovine blood serum albumin. Specific antipeptide antibodies were isolated and purified using immunosorbent involving the antigen--lactate dehydrogenase-5. Immunodiffusion procedure and immunoenzymatic assay showed that the antipeptide antibodies obtained were specific towards conjugate, N-terminal peptide and the native pig muscle lactate dehydrogenase-5. The antipeptide antibodies towards N-terminal fragment of lactate dehydrogenase did not affect the enzymatic activity.
Alekseeva
A E
AE
Potapkina
T A
TA
Prozorovskiĭ
V N
VN
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000276:immunology*; D009132:Muscles / Q000201:enzymology*; D010455:Peptides / Q000276:immunology*; D013552:Swine
D000906:Antibodies; D007527:Isoenzymes; D010455:Peptides; D007770:L-Lactate Dehydrogenase
Antipeptidnye antitela, spetsifichnye k N-kontsevomu raĭonu LDG myshtsy svin'i.