Synthesis of porcine brain natriuretic peptide-32 using silver tetrafluoroborate as a new deprotecting reagent of the S-trimethylacetamidomethyl group.
Silver tetrafluoroborate (AgBF4) in trifluoroacetic acid (TFA) has been found to cleave the S-trimethylacetamidomethyl (Tacm) group or the S-acetamidomethyl (Acm) group without affecting other functional groups in the peptide chain. Newly isolated porcine brain natriuretic peptide-32 (pBNP-32) was synthesized using AgBF4 and Cys(Tacm) derivatives. The synthetic pBNP-32 had the highest chick rectum relaxant activity among the ANP-BNP families.
Yoshida
M
M
Department of Medicinal Chemistry, Kyoto Pharmaceutical University, Japan.
Akaji
K
K
Tatsumi
T
T
Iinuma
S
S
Fujiwara
Y
Y
Kimura
T
T
Kiso
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001881:Borates; D001923:Brain Chemistry; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D020097:Natriuretic Peptide, Brain; D009419:Nerve Tissue Proteins / Q000138:chemical synthesis*; D012834:Silver; D018030:Silver Compounds; D013552:Swine
D001881:Borates; D007202:Indicators and Reagents; D009419:Nerve Tissue Proteins; D018030:Silver Compounds; D020097:Natriuretic Peptide, Brain; C064097:silver tetrafluoroborate; D012834:Silver