Pradimicin A inhibition of human immunodeficiency virus: attenuation by mannan.
The mechanism of action of a novel anti-HIV antibiotic, pradimicin A, has been studied using cell-free (MT-4 cells) and cell to cell (MOLT-4 and MOLT-4/HIVHTLV-3B cells) HIV infection systems. The data indicate that (1) preincubation of the cells with HIV at 0 degrees for 1 hr, followed by the addition of pradimicin A and further incubation at 37 degrees, resulted in a complete inhibition of infection while preincubation at 37 degrees did not. Similar data were obtained with the coculture between MOLT-4 and MOLT-4/HIV cells. (2) Virus treated with pradimicin A followed by washing did not show any inhibition on subsequent HIV binding to cells. (3) The inhibitory effect of pradimicin A on HIV infection was prevented by mannan but not by other sugars tested. Mannan, however, did not interfere with the anti-HIV activity of other known inhibitors. (4) Use of EGTA suggested that pradimicin A required Ca ions to exert its anti-HIV activity. These data imply that pradimicin A inhibits an early step in HIV infection, probably through its binding to mannose residues of HIV glycoprotein.
Tanabe-Tochikura
A
A
Department of Virology and Parasitology, Yamaguchi University School of Medicine, Japan.
Tochikura
T S
TS
Yoshida
O
O
Oki
T
T
Yamamoto
N
N
D018943:Anthracyclines; D000903:Antibiotics, Antineoplastic / Q000378:metabolism / Q000494:pharmacology*; D000998:Antiviral Agents / Q000378:metabolism / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D003080:Cold Temperature; D003588:Cytopathogenic Effect, Viral; D006678:HIV / Q000187:drug effects*; D006801:Humans; D008351:Mannans / Q000378:metabolism / Q000494:pharmacology*; D000095643:Pradimicins and Benanomicins
D018943:Anthracyclines; D000903:Antibiotics, Antineoplastic; D000998:Antiviral Agents; D002118:Calcium; D008351:Mannans; C058036:pradimicin A; D000095643:Pradimicins and Benanomicins