Corticotrophin-releasing factor-like immunoreactivity and mRNA in human leukocytes.
Unstimulated normal leukocytes were studied for their expression of corticotrophin-releasing factor (CRF)-like mRNA and peptide. In situ hybridization demonstrated CRF mRNA in T and B lymphocytes and in neutrophils. Northern blot analysis also detected the presence of a CRF mRNA species of approximately 1.7 Kb from lymphocytes and a peptide that reacted with anti-CRF antiserum was also detected by radioimmunoassay. This lymphocyte-derived peptide eluted earlier than standard CRF on high performance liquid chromatography. These findings suggest that regulatory mechanisms within the hypothalamus and the immune system may utilize closely related peptides.
Stephanou
A
A
Medical Unit, Charing Cross and Westminster Medical School, London, England.
Jessop
D S
DS
Knight
R A
RA
Lightman
S L
SL
D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D003346:Corticotropin-Releasing Hormone / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D011863:Radioimmunoassay; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*
D012333:RNA, Messenger; D003346:Corticotropin-Releasing Hormone