title: N-(9-acridinyl)-bromoacetamide--a powerful reagent for phase-transfer-catalyzed fluorescence labeling of carboxylic acids for liquid chromatography.
abstract: Phase-transfer-catalyzed esterification of various carboxylic acids has been carried out on a microanalytical scale with a new reagent, N-(9-acridinyl)-bromoacetamide, in aqueous/organic two-phase systems. The procedure gives esters showing very high fluorescence intensity in acid solution. The large Stokes' shift (greater than 120 nm) means that a low background can be achieved even when using a large emission bandwidth. These derivatives show very good chromatographic behavior on reversed-phase liquid chromatographic columns with the use of aqueous acetonitrile, containing 0.2% of phosphoric acid, as the mobile phase. Detection limits (S/N = 2) as low as 10 fmol can be obtained under optimal conditions.
authors.affiliation: Laboratory of Microbiological Chemistry, University of Gothenburg, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Allenmark
authors.forename: M
authors.initials: M
authors.lastname: Chelminska-Bertilsson
authors.forename: R A
authors.initials: RA
authors.lastname: Thompson
mesh_terms: D000609:Aminoacridines; D001647:Bile Acids and Salts / Q000032:analysis; D002264:Carboxylic Acids / Q000032:analysis*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002853:Chromatography, Liquid / Q000379:methods; D004951:Esterification; D004988:Ethers, Cyclic / Q000032:analysis; D019319:Okadaic Acid; D013050:Spectrometry, Fluorescence
chemical_list: D000609:Aminoacridines; D001647:Bile Acids and Salts; D002264:Carboxylic Acids; D004988:Ethers, Cyclic; C064158:N-(9-acridinyl)bromoacetamide; D019319:Okadaic Acid