title: Evidence for a factor protective against hypoxic liver parenchymal cell injury in a protein-free blood extract.
abstract: The effect of a protein-free extract from calf blood on hypoxic cell injury was studied in experiments with primary cultures of isolated hepatocytes. At concentrations above 1 mg/ml the extract produced marked protection. At 4 h of anaerobic incubation at 37 degrees C only 20% of cells were damaged as compared to 50% of the anaerobic control incubation. These data demonstrate the presence of a factor in the blood extract which protects against hypoxic cell injury.
authors.affiliation: Klinische Forschergruppe Leberschädigung, Institut für Physiologische Chemie I, Heinrich-Heine-Universität Düsseldorf, West Germany.
authors.forename: H
authors.initials: H
authors.lastname: de Groot
authors.forename: M
authors.initials: M
authors.lastname: Brecht
authors.forename: F
authors.initials: F
authors.lastname: Machicao
mesh_terms: D000818:Animals; D015687:Cell Hypoxia / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006418:Heme / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014343:Trypan Blue
chemical_list: D006418:Heme; C015646:Actovegin; D007770:L-Lactate Dehydrogenase; D014343:Trypan Blue