title: Selective inhibitors of KCl cotransport in human red cells.
abstract: Two analogues of the loop diuretics furosemide and bumetanide have been identified as differential inhibitors of KCl and NaKCl cotransport systems, assayed by measuring K+ influx in 'young' human red cells. H25 inhibited both NaKCl and KCl cotransport, with I50% values of 0.03 and 30 microM respectively; H74 had no effect on NaKCl cotransport, even at 0.3 mM, but inhibited KCl cotransport with an I50% of 75 microM. These compounds are therefore useful for resolving the two transport systems.
authors.affiliation: Department of Physiology, University of Oxford, UK.
authors.forename: J C
authors.initials: JC
authors.lastname: Ellory
authors.forename: A C
authors.initials: AC
authors.lastname: Hall
authors.forename: S O
authors.initials: SO
authors.lastname: Ody
authors.forename: H C
authors.initials: HC
authors.lastname: Englert
authors.forename: D
authors.initials: D
authors.lastname: Mania
authors.forename: H J
authors.initials: HJ
authors.lastname: Lang
mesh_terms: D000755:Anemia, Sickle Cell / Q000150:complications / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D002034:Bumetanide / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D005665:Furosemide / Q000494:pharmacology; D006432:Hemochromatosis / Q000150:complications / Q000378:metabolism; D006801:Humans; D011189:Potassium Chloride / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism
chemical_list: D002034:Bumetanide; D011189:Potassium Chloride; D005665:Furosemide; D012964:Sodium