Inhibition of phytohemagglutinin-stimulated bovine mononuclear cell proliferation, interleukin-2 production and protein kinase C activity by a protein kinase C inhibitor, H-7.
1-(5-Isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride (H-7), a potent and selective inhibitor of protein kinase C (PKC), inhibited PHA-stimulated bovine peripheral blood mononuclear cell (PBMC) proliferation, interleukin-2 (IL-2) production, and cytosolic PKC activity without affecting the cell viabilities. Presence of exogenous cytokines, such as purified human IL-2 or recombinant bovine IL-2 (rbovIL-2), reversed the H-7 inhibitory effects on PHA-stimulated PBMC proliferation. We conclude that the PKC enzyme plays an important role as a second messenger in bovine PBMC proliferation in the early stages of cell activation.
Atluru
D
D
Department of Anatomy, Kansas State University, Manhattan 66506.
Polam
S
S
Sarraju
L
L
Blecha
F
F
Minocha
H C
HC
Atluru
S
S
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000704:Analysis of Variance; D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003600:Cytosol / Q000201:enzymology; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000494:pharmacology; D007546:Isoquinolines / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology; D008213:Lymphocyte Activation; D010835:Phytohemagglutinins / Q000276:immunology; D010879:Piperazines / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D011994:Recombinant Proteins; D013449:Sulfonamides
D007376:Interleukin-2; D007546:Isoquinolines; D010835:Phytohemagglutinins; D010879:Piperazines; D011994:Recombinant Proteins; D013449:Sulfonamides; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; C043204:N-(2-guanidinoethyl)-5-isoquinolinesulfonamide; D011493:Protein Kinase C