Direct DNA sequencing of PCR amplified genomic DNA by the Maxam-Gilbert method.
We present a method to directly sequence PCR amplification products utilizing the chemical method of Maxam and Gilbert. This procedure yields clearly readable DNA sequences of 100-400 base pairs in length derived from human genomic DNA in four days' time.
Tahara
T
T
Yale University School of Medicine, Department of Human Genetics, New Haven, CT 06510.
Kraus
J P
JP
Rosenberg
L E
LE
D001483:Base Sequence; D004247:DNA / Q000302:isolation & purification*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction
D004247:DNA