Effect of diet and substrate on the in vitro measurement of cholesterol and fatty-acid synthesis in hepatic tissue of Japanese quail.
A comparison of acetate, glucose, mevalonate, or water as radioactive substrates for the hepatic synthesis of cholesterol, fatty acids, and glyceride-glycerol was made in Japanese quail fed diets containing either beef fat or tuna oil. The quail fed a diet containing beef fat were fatter and had a significantly higher (P less than .01) concentration of serum cholesterol (5.6 mM per L) than that measured in the serum of quail given tuna oil (4.1 mM per L). Both in vitro cholesterol and fatty-acid synthesis were greater in the quail fed a diet of beef fat than in those fed a diet containing tuna oil. The results showed that mevalonate was the most-suitable radioactive substrate for measuring cholesterol synthesis, whereas glucose was the most-suitable radioactive substrate for measuring fatty-acid and glyceride-glycerol synthesis.
Hood
R L
RL
CSIRO, Australia, Division of Food Processing, North Ryde, New South Wales, Australia.
D000818:Animals; D002417:Cattle; D002784:Cholesterol / Q000096:biosynthesis*; D003370:Coturnix / Q000378:metabolism*; D004041:Dietary Fats / Q000378:metabolism*; D005227:Fatty Acids / Q000096:biosynthesis* / Q000378:metabolism; D005395:Fish Oils / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D011784:Quail / Q000378:metabolism*; D014413:Tuna
D004041:Dietary Fats; D005227:Fatty Acids; D005395:Fish Oils; D002784:Cholesterol