[Relation between DNA replication and neutral Mn 2+-dependent DNAse activity in chromatin].
In cultures of primary murine fibroblasts the 10% serum stimulates the replicative synthesis of DNA inhibited by aphidicolin and araC (cytosine arabinoside). Using direct immunofluorescence analysis, it was shown that antibodies penetrate inside the cells and after 4 hours are pooled in the nuclei, where they remain for another 20 hours. The substitution of antibodies against chromatin DNAase by bovine serum albumin of normal serum gamma-globulins does not interfere with the DNA synthesis induction.
Vinter
V G
VG
Askarova
A N
AN
Zotkina
N L
NL
Kulikov
V V
VV
Drize
O B
OB
Shliankevich
M A
MA
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002478:Cells, Cultured; D002843:Chromatin / Q000201:enzymology* / Q000378:metabolism; D004261:DNA Replication; D003851:Deoxyribonucleases / Q000276:immunology / Q000378:metabolism*; D005347:Fibroblasts / Q000201:enzymology / Q000378:metabolism; D007114:Immunization; D008099:Liver / Q000201:enzymology; D008345:Manganese / Q000378:metabolism*; D051379:Mice
D000906:Antibodies; D002843:Chromatin; D008345:Manganese; D003851:Deoxyribonucleases
Zavisimost' replikatsii DNK ot aktivnosti neĭtral'noĭ Mn 2+-zavisimoĭ DNKazy khromatina.