title: Three types of GABA-immunoreactive cells in the lamprey spinal cord.
abstract: Polyclonal antisera raised against conjugated GABA were used to study the distribution of GABAergic neurons in the spinal cords of lampreys (Lampetra fluviatilis and Ichtyomyzon unicuspis) using immunofluorescence and peroxidase-antiperoxidase techniques. Three morphologically distinct types of GABA-immunoreactive (GABA-ir) cell bodies were observed, multipolar neurons in the lateral grey cell column, apparently bipolar cells in the ventral aspect of the dorsal horn, and small liquor-contacting cells surrounding the central canal. A high density of immunoreactive fibers of spinal origin were present in the lateral and ventral funiculi, whereas the dorsal column had a relatively low density. Dense GABA-ir plexuses were situated in the lateral spinal margin, and in the dorsal part of the dorsal horn. A chronic lesion of the rostral spinal cord did not result in any observable loss of GABA-ir fibers below or above the lesion, suggesting that the 3 types of segmental GABA-ir neurons are the main sources of the GABAergic innervation of the lamprey spinal cord.
authors.affiliation: Nobel Institute for Neurophysiology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Brodin
authors.forename: N
authors.initials: N
authors.lastname: Dale
authors.forename: J
authors.initials: J
authors.lastname: Christenson
authors.forename: J
authors.initials: J
authors.lastname: Storm-Mathisen
authors.forename: T
authors.initials: T
authors.lastname: Hökfelt
authors.forename: S
authors.initials: S
authors.lastname: Grillner
mesh_terms: D000818:Animals; D005399:Fishes / Q000378:metabolism*; D007150:Immunohistochemistry; D007798:Lampreys / Q000378:metabolism*; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D005680:gamma-Aminobutyric Acid