A protein of neutrophil granules interferes with activation of NADPH oxidase in a cell-free system.
A soluble extract of neutrophil granules interfered with activation of the NADPH oxidase in a cell-free system. The extract had no effect on superoxide production by preactivated enzyme. The inhibitory activity was retained during dialysis and was lost upon exposure to proteinase K indicating that the active substance was a protein. The inhibitor exhibited a high stability at elevated temperatures. Chromatography of granules extract on ion exchangers implied that the inhibitor was a positively charged protein eluting from S Sepharose cation exchanger above 0.4M concentration of NaCl.
Aviram
I
I
Department of Biochemistry, Faculty of Life Sciences, Tel-Aviv University, Israel.
Faber
A
A
D002457:Cell Extracts / Q000032:analysis / Q000494:pharmacology*; D002474:Cell-Free System; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D009247:NADH, NADPH Oxidoreductases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D019255:NADPH Oxidases; D009504:Neutrophils; D011506:Proteins / Q000032:analysis; D014020:Tissue Extracts / Q000494:pharmacology*
D002457:Cell Extracts; D011506:Proteins; D014020:Tissue Extracts; D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases