title: Budding process and maturation of human immunodeficiency virus examined by means of pre- and post-embedding immunocolloidal gold electron microscopy.
abstract: The techniques of pre- and post-embedding immunocolloidal gold electron microscopy were tested for their usefulness for analyzing the morphogenesis of human immunodeficiency virus (HIV) strain LAV employing U-937 cells persistently infected with the virus (U-937/LAV). By both techniques the following results were obtained. (1) Budding process was restricted to a localized area at the membrane adjacent to the Golgi apparatus. (2) The distribution of viral envelope antigens was restricted to the electron-dense crescent structures on the cell surface. (3) Virions released from the cells could be classified mainly into 2 categories: virions with doughnut-shaped cores or with conical cores at the center of the particles. And p24 proteins localized on the thick electron-dense cores of both types. These findings support the idea that pre- and post-embedding immunogold electron microscopy is very useful for studying the morphogenesis of viruses.
authors.affiliation: Department of Virology, Tottori University School of Medicine, Yonago, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Katsumoto
authors.forename: M
authors.initials: M
authors.lastname: Asanaka
authors.forename: S
authors.initials: S
authors.lastname: Kageyama
authors.forename: T
authors.initials: T
authors.lastname: Kurimura
authors.forename: K
authors.initials: K
authors.lastname: Nakajima
authors.forename: A
authors.initials: A
authors.lastname: Noto
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
authors.forename: R
authors.initials: R
authors.lastname: Sato
mesh_terms: D006678:HIV / Q000254:growth & development / Q000648:ultrastructure*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008854:Microscopy, Electron / Q000379:methods