title: Antigenic cross-reactivity of venoms obtained from snakes of genus Bothrops.
abstract: Antigenic cross-reactivity was studied among the components of venoms from nine species of the genus Bothrops using species-specific antivenoms. Sera titration by DOT-ELISA detected similar levels of antibody when either homologous or heterologous antigens were used. Transblotted antigens, after SDS-PAGE fractionation, were also revealed by homologous and heterologous antivenoms. Antigens with mol. wt greater than 30,000 seemed to be the most cross-reactive. Antigens of about 24,000 mol. wt were poorly immunogenic. Antigens between 14-18,000 mol. wt cross-reacted only with B. moojeni, B. jararacussu, B. neuwiedi and B. pradoi venoms. Neutralization of the lethality of B. jararaca venom was observed by homologous and heterologous antivenoms.
authors.affiliation: Centro de Imunologia, Instituto Butantan, São Paulo, Brazil.
authors.forename: A M
authors.initials: AM
authors.lastname: da Silva
authors.forename: M R
authors.initials: MR
authors.lastname: Lima
authors.forename: A K
authors.initials: AK
authors.lastname: Nishikawa
authors.forename: C I
authors.initials: CI
authors.lastname: Brodskyn
authors.forename: M C
authors.initials: MC
authors.lastname: dos Santos
authors.forename: M F
authors.initials: MF
authors.lastname: Furtado
authors.forename: W D
authors.initials: WD
authors.lastname: da Silva
authors.forename: I
authors.initials: I
authors.lastname: Mota
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D000941:Antigens / Q000032:analysis*; D015153:Blotting, Western; D003429:Cross Reactions; D003435:Crotalid Venoms / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D051379:Mice; D008805:Mice, Inbred A; D008970:Molecular Weight; D009500:Neutralization Tests
chemical_list: D000941:Antigens; D003435:Crotalid Venoms