Changes in cardiac output, hepatic and renal functions in rats with isoprenaline-induced heart damage.
A model of acute rat heart damage by a large single dose of isoprenaline (ISO) (100 mg.kg-1 s.c.) was assessed for its suitability to evaluate changes in drug kinetics in acute heart failure. Within 24h after the administration of the above dose the colorimetric dilution method revealed a decrease in cardiac output by more than 20% and mild increase in total peripheral resistance in anaesthetized female Wistar rats. At the same time, marked hypotension developed with a decrease in mean arterial blood pressure by nearly 20%. An acceleration of both depolarization and repolarization of the atrial and ventricular musculatures and a surprising bradycardia were demonstrated electrocardiographically. ECG failed to demonstrate any signs of myocardial ischaemia. Determination of bromsulphalein and inulin clearance values revealed decreased liver and kidney function. Mild anaesthesia-induced respiratory and metabolic acidosis was improved by ISO administration. The described model at least partially corresponds to the manifestations of acute heart failure with hypotension in man.
Hrdina
R
R
Institute of Experimental Biopharmaceutics, Czechoslovak Academy of Sciences, Hradec Králové.
Kvĕtina
J
J
D000818:Animals; D002302:Cardiac Output; D005260:Female; D006207:Half-Life; D006333:Heart Failure / Q000139:chemically induced / Q000503:physiopathology*; D007444:Inulin; D007545:Isoproterenol; D007668:Kidney / Q000503:physiopathology*; D008099:Liver / Q000503:physiopathology*; D051381:Rats; D011919:Rats, Inbred Strains; D013448:Sulfobromophthalein
D013448:Sulfobromophthalein; D007444:Inulin; D007545:Isoproterenol