title: Cyclobutane-pyrimidine dimer excision in UV-sensitive CHO mutants and the effect of the human ERCC2 repair gene.
abstract: Using a radiochromatographic assay, we have examined cis-syn cyclobutane-pyrimidine dimer removal after ultraviolet irradiation in cell lines representative of the first 6 complementation groups of Chinese hamster ovary DNA nucleotide excision repair mutants. AA8, the CHO cell line from which these mutants were derived, consistently showed normal dimer excision for a rodent cell. The mutants uniformly exhibited no significant dimer excision within the limits of determination. Additionally, V-H1, a mutant belonging to complementation group 2 and derived from V79 hamster cells, exhibited no dimer excision. Two UV5 derived transformants that carry the complementing human ERCC2 repair gene showed a capacity for dimer excision comparable to the AA8 wild-type cells.
authors.affiliation: Biology Division, Oak Ridge National Laboratory, TN 37831.
authors.forename: J D
authors.initials: JD
authors.lastname: Regan
authors.forename: L H
authors.initials: LH
authors.lastname: Thompson
authors.forename: W L
authors.initials: WL
authors.lastname: Carrier
authors.forename: C A
authors.initials: CA
authors.lastname: Weber
authors.forename: A A
authors.initials: AA
authors.lastname: Francis
authors.forename: M Z
authors.initials: MZ
authors.lastname: Zdzienicka
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004260:DNA Repair; D004269:DNA, Bacterial / Q000528:radiation effects*; D005796:Genes; D006801:Humans; D009154:Mutation; D011740:Pyrimidine Dimers / Q000235:genetics*; D014170:Transformation, Genetic; D014466:Ultraviolet Rays
chemical_list: D004269:DNA, Bacterial; D011740:Pyrimidine Dimers