Isolation and characterization of a cDNA for rat liver cysteine dioxygenase.
Cysteine dioxygenase is a key enzyme of cysteine metabolism in mammals. The cDNA clones for rat liver cysteine dioxygenase were isolated by immunological screening and plaque hybridization from a rat liver cDNA library. The longest clone contained an insert of 1458 bp and encoded a polypeptide of 200 amino acids. The clone included the corresponding nucleotide sequence to amino acid sequences obtained from four lysyl endopeptidase-digested fragments of purified rat liver cysteine dioxygenase. The calculated molecular weight of rat liver cysteine dioxygenase was 23,025. Northern blot analysis revealed a single cysteine dioxygenase mRNA species of about 1.7 kb. A computer homology search indicated that this protein showed no homology with any known protein.
Hosokawa
Y
Y
National Institute of Health and Nutrition, Tokyo, Japan.
Matsumoto
A
A
Oka
J
J
Itakura
H
H
Yamaguchi
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003545:Cysteine / Q000378:metabolism; D050558:Cysteine Dioxygenase; D004247:DNA / Q000032:analysis*; D049308:Dioxygenases; D008099:Liver / Q000201:enzymology*; D008297:Male; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D015183:Restriction Mapping
D012333:RNA, Messenger; D004247:DNA; D010105:Oxygenases; D049308:Dioxygenases; D050558:Cysteine Dioxygenase; D003545:Cysteine