A simplified competition data analysis for radioligand specific activity determination.
Non-linear regression and two-step linear fit methods were developed to determine the actual specific activity of 125I-ovine prolactin by radioreceptor self-displacement analysis. The experimental results obtained by the different methods are superposable. The non-linear regression method is considered to be the most adequate procedure to calculate the specific activity, but if its software is not available, the other described methods are also suitable.
Venturino
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Cátedra de Física, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
Rivera
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Bergoc
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RM
Caro
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D000818:Animals; D005260:Female; D007457:Iodine Radioisotopes; D011388:Prolactin / Q000032:analysis; D011869:Radioligand Assay / Q000379:methods*; D051381:Rats; D011919:Rats, Inbred Strains; D012044:Regression Analysis; D012756:Sheep
D007457:Iodine Radioisotopes; D011388:Prolactin