Presentation of viral antigen controlled by a gene in the major histocompatibility complex.
We describe a mutant human cell line (LBL 721.174) that has lost a function required for presentation of intracellular viral antigens with class I molecules of the major histocompatibility complex (MHC), but retains the capacity to present defined epitopes as extracellular peptides. The cell also has a defect in the assembly and expression of class I MHC molecules, which we show can be restored by exposure of the cells to a peptide epitope. This phenotype suggests a defect in the association of intracellular antigen with class I molecules similar to that described for the murine mutant RMA-S (ref. 5), but in the present case the genetic defect can be mapped within the MHC locus on human chromosome 6.
Cerundolo
V
V
Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, UK.
Alexander
J
J
Anderson
K
K
Lamb
C
C
Cresswell
P
P
McMichael
A
A
Gotch
F
F
Townsend
A
A
D000938:Antigen-Presenting Cells / Q000502:physiology*; D000956:Antigens, Viral / Q000276:immunology*; D002460:Cell Line; D002896:Chromosomes, Human, Pair 6; D015395:Histocompatibility Antigens Class I / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D009980:Influenza A virus / Q000276:immunology; D008285:Major Histocompatibility Complex; D009842:Oligopeptides / Q000378:metabolism; D011233:Precipitin Tests; D014763:Viral Matrix Proteins / Q000276:immunology
D000956:Antigens, Viral; D015395:Histocompatibility Antigens Class I; D009842:Oligopeptides; D014763:Viral Matrix Proteins