Unusually high rates of metabolism of DHT in cytosols of the quail uropygial gland.
The metabolism of DHT in the cytosol of the quail uropygial gland was found to be so high that the steroid was almost completely inactivated within 2 hours of incubation at 0 C. In these conditions, DHT cannot be used for the characterization of androgen receptors. By contrast, R 1881 and mibolerone, which are not metabolized, can be used as alternative ligands. Moreover, the extremely high metabolism of DHT questions the physiologic role of this steroid in the quail uropygial gland.
Amet
Y
Y
Laboratoire de Biochimie, UA 598 du CNRS, Faculté de Médecine, Brest, France.
di Stefano
S
S
Quemener
E
E
Abalain
J H
JH
Floch
H H
HH
Daniel
J Y
JY
D000818:Animals; D003600:Cytosol / Q000378:metabolism*; D013196:Dihydrotestosterone / Q000378:metabolism*; D008297:Male; D015741:Metribolone / Q000378:metabolism; D009277:Nandrolone / Q000031:analogs & derivatives / Q000378:metabolism; D011784:Quail / Q000378:metabolism*; D011944:Receptors, Androgen / Q000378:metabolism; D012627:Sebaceous Glands / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism; D045165:Testosterone Congeners / Q000378:metabolism
D011944:Receptors, Androgen; D045165:Testosterone Congeners; D013196:Dihydrotestosterone; D015741:Metribolone; D013739:Testosterone; D009277:Nandrolone; C100075:mibolerone