title: Production of highly specific anti-testosterone antiserum by an immunotolerance method.
abstract: Anti-testosterone antisera were produced by pretreatment of rabbits with 15 alpha-carboxymethyl-5 alpha-dihydrotestosterone linked to a copolymer of D-glutamic acid and D-lysine (D-GL) before immunization with the bovine serum albumin conjugate of 15 alpha- and 15 beta-carboxymethyltestosterone. The specificity for 5 alpha-dihydrotestosterone of the antitestosterone antisera was considerably improved.
authors.affiliation: Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Miyake
authors.forename: M
authors.initials: M
authors.lastname: Shigetoshi
authors.forename: M
authors.initials: M
authors.lastname: Kojima
mesh_terms: D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000918:Antibody Specificity; D007108:Immune Tolerance; D066298:In Vitro Techniques; D011817:Rabbits; D013739:Testosterone / Q000276:immunology*
chemical_list: D000906:Antibodies; D013739:Testosterone