title: A Drosophila ribosomal protein functions in mammalian cells.
abstract: A cDNA expression vector encoding Drosophila ribosomal protein S14 was transfected into cultured Chinese hamster ovary (CHO) cells that harbor a recessive RPS14 emetine resistance mutation. Transformants synthesized the insect mRNA and polypeptide and consequently displayed an emetine-sensitive phenotype. These observations indicate that the insect protein was accurately expressed and correctly assembled into functional mammalian 40S ribosomal subunits.
authors.affiliation: Division of Biology, Kansas State University, Manhattan 66506.
authors.forename: C G
authors.initials: CG
authors.lastname: Maki
authors.forename: D D
authors.initials: DD
authors.lastname: Rhoads
authors.forename: J J
authors.initials: JJ
authors.lastname: Diaz
authors.forename: D J
authors.initials: DJ
authors.lastname: Roufa
mesh_terms: D000818:Animals; D002460:Cell Line; D004330:Drosophila / Q000235:genetics*; D015183:Restriction Mapping; D012269:Ribosomal Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D012269:Ribosomal Proteins; C039747:ribosomal protein S14