title: Characterization of Coxiella burnetii pyrB.
abstract: The C. burnetii pyrB gene was cloned on a 7-kbp EcoR I fragment. DNA sequence analysis, enzyme assays, and amino acid homologies with E. coli and B. subtilis pyrB gene products suggest that (i) C. burnetii ATCase exists as a trimer, (ii) the microorganism may not synthesize a regulatory polypeptide, and (iii) pyrB may be part of an operon whose expression is under the control of an upstream promoter. The high degree of homology of the active site further suggests that a common mechanism of catalysis for ATCase exists between such diverse organisms as C. burnetii, E. coli, and B. subtilis.
authors.affiliation: United States Army Medical Research Institute of Infectious Diseases, Department of Intracellular Pathogens, Fort Detrick, Frederick, Maryland 21701-5011.
authors.forename: T A
authors.initials: TA
authors.lastname: Hoover
authors.forename: J C
authors.initials: JC
authors.lastname: Williams
mesh_terms: D000595:Amino Acid Sequence; D001221:Aspartate Carbamoyltransferase / Q000032:analysis / Q000235:genetics*; D003001:Cloning, Molecular; D003381:Coxiella / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data
chemical_list: D001221:Aspartate Carbamoyltransferase