A procedure for enrichment and isolation of mutants of the salt-tolerant yeast Debaryomyces hansenii having altered glycerol metabolism.
The salt-tolerant yeast Debaryomyces hansenii produces and accumulates glycerol when subjected to salt stress, whereby the buoyant density of the cells is changed. This property allows for enrichment of mutants with altered glycerol metabolism by density gradient centrifugation. Colonies derived from cells with rapidly changing density following an osmotic shock were screened for increased glycerol production by observing their ability to support growth of a glycerol-requiring strain of Escherichia coli. The glycerol overproducting phenotype of two isolates was confirmed by chemical analysis.
Morales
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Department of General and Marine Microbiology, University of Göteborg, Sweden.
André
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Adler
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D002499:Centrifugation, Density Gradient; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005800:Genes, Fungal; D005990:Glycerol / Q000378:metabolism*; D007621:Karyotyping; D009154:Mutation; D004718:Saccharomycetales / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*
D004271:DNA, Fungal; D012965:Sodium Chloride; D005990:Glycerol