Stimulatory and inhibitory effects of serum and conditioned medium on cell spreading.
This work deals with the effects of coating culture dishes with chick embryo fibroblast conditioned medium (CM) and 1% fetal calf serum (FCS) on the rates of attachment and spreading of chick embryo fibroblasts. It appeared that a FCS coating over a CM precoating exerted a remarkable promoting cooperative effect on cell spreading whereas a CM coating over a FCS precoating had a very marked inhibitory effect. Precoating with cobalt-protoporphyrin IX (CoPP) was also performed to serve as a substrate for FCS or CM coating. This CoPP precoating was found to exert a promoting effect for FCS coating only.
Verger
C
C
C.N.R.S., UPR 303 I.R.S.C., Villejuif, France.
Imbenotte
J
J
D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002465:Cell Movement / Q000502:physiology*; D002642:Chick Embryo; D003470:Culture Media; D005347:Fibroblasts / Q000502:physiology; D066298:In Vitro Techniques; D010949:Plasma / Q000502:physiology; D011166:Porphyrins / Q000502:physiology*; D011524:Protoporphyrins / Q000502:physiology*
D003470:Culture Media; D011166:Porphyrins; D011524:Protoporphyrins; C028025:protoporphyrin IX