title: High-performance liquid chromatographic analysis of imidazolium and pyridinium oximes in plasma and urine.
abstract: Imidazolium oximes are useful in the treatment of organophosphorus agent poisoning. However, the extant methods for analyzing oximes in plasma and urine samples are not adequate. A unique high-performance liquid chromatographic assay method was developed for quantitating either imidazolium or pyridinium oximes. Plasma or urine samples can be injected directly onto the column after a centrifugation filtration step. This method demonstrates a different approach in the method development for quaternary ammonium compounds using non-end-capped reversed-phase columns and a mobile phase containing competing cations. In addition, a preliminary pharmacokinetic study of the imidazolium oxime in rabbits was carried out using this method.
authors.affiliation: U.S. Army Medical Research Institute of Chemical Defense, Aberdeen Proving Ground, MD 21010-5425.
authors.forename: M L
authors.initials: ML
authors.lastname: Shih
authors.forename: J R
authors.initials: JR
authors.lastname: Smith
authors.forename: G W
authors.initials: GW
authors.lastname: Salem
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006207:Half-Life; D007093:Imidazoles / Q000032:analysis* / Q000097:blood / Q000652:urine; D008832:Microchemistry; D010091:Oximes / Q000032:analysis* / Q000097:blood / Q000652:urine; D011726:Pyridinium Compounds / Q000032:analysis* / Q000097:blood / Q000652:urine; D011786:Quality Control; D011817:Rabbits
chemical_list: D007093:Imidazoles; D010091:Oximes; D011726:Pyridinium Compounds; C065159:WR 254416; C065160:WR 255737; C005514:N,N'-monomethylenebis(pyridiniumaldoxime); C022870:asoxime chloride