A method for the microanalysis of hexoses in glycoproteins.
A procedure has been developed by which less than micrograms quantities of hexoses in glycoproteins can be determined by g.l.c.-m.s. with selected-ion monitoring of the alditol acetates derived from the sugars released by acid hydrolysis. The effectiveness of the method was demonstrated by determination of the hexose and hexosamine composition of 5-micrograms samples of antithrombin III and von Willebrand factor, respectively.
Kenne
L
L
Department of Organic Chemistry, Arrhenius Laboratory, University of Stockholm, Sweden.
Strömberg
S
S
D000990:Antithrombin III; D002240:Carbohydrate Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D008401:Gas Chromatography-Mass Spectrometry; D006023:Glycoproteins; D006595:Hexosamines / Q000032:analysis; D006601:Hexoses / Q000032:analysis*; D008969:Molecular Sequence Data; D013402:Sugar Alcohols / Q000032:analysis; D014841:von Willebrand Factor
D006023:Glycoproteins; D006595:Hexosamines; D006601:Hexoses; D013402:Sugar Alcohols; D014841:von Willebrand Factor; D000990:Antithrombin III