Phosphatidylserine-mediated delivery of cholesterol to macrophages: a novel experimental method for the generation of foam cells.
P388D1 macrophages were incubated for 24 h with cholesterol-phosphatidylserine liposomes (50 micrograms cholesterol/ml) and the content of cellular cholesteryl esters increased to approx. 200 micrograms/mg cell protein. Similar results were not observed with cholesterol-phosphatidylcholine liposomes. These results demonstrate that specific phospholipid-cholesterol liposomes can be utilized for the experimental production of macrophage cholesterol-rich foam cells.
Greenspan
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Department of Pathology, University of South Carolina, School of Medicine, Columbia 29208.
D000818:Animals; D002460:Cell Line; D002784:Cholesterol / Q000008:administration & dosage / Q000378:metabolism*; D002788:Cholesterol Esters / Q000096:biosynthesis; D004337:Drug Carriers; D007700:Kinetics; D008081:Liposomes; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D010718:Phosphatidylserines
D002788:Cholesterol Esters; D004337:Drug Carriers; D008081:Liposomes; D010718:Phosphatidylserines; D002784:Cholesterol