Ascorbate as a source of reducing equivalents for the synthesis of aldosterone.
The three steps in the synthesis of aldosterone (11 beta/18-hydroxylations and aldehyde synthetase) were examined in mitochondria from bovine glomerulosa and fasciculata to study the regulation of aldehyde synthetase. Ascorbate plus NADH shows synergism with malate in stimulating aldehyde synthetase without affecting 11 beta/-18-hydroxylations. The concentration of semidehydroascorbate reductase in mitochondria from glomerulosa is more than twice that from fasciculata. We propose that in glomerulosa, ascorbate provides a source of reducing equivalents that specifically support the last step in the synthesis of aldosterone.
Yanagibashi
K
K
Department of Pharmacology I, Jikei University School of Medicine, Tokyo, Japan.
Kobayashi
Y
Y
Hall
P F
PF
D000311:Adrenal Glands / Q000187:drug effects / Q000201:enzymology*; D000445:Aldehyde Oxidoreductases / Q000378:metabolism*; D000450:Aldosterone / Q000096:biosynthesis*; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism* / Q000494:pharmacology; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003900:Desoxycorticosterone / Q000378:metabolism; D004347:Drug Interactions; D008293:Malates / Q000494:pharmacology; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology*; D009243:NAD / Q000494:pharmacology; D009249:NADP / Q000494:pharmacology
D008293:Malates; D009243:NAD; D003900:Desoxycorticosterone; D000450:Aldosterone; D009249:NADP; C030298:malic acid; D003577:Cytochrome P-450 Enzyme System; D000445:Aldehyde Oxidoreductases; D001205:Ascorbic Acid