Binding of substrates to cytochrome P450 enzymes: mathematical artifacts in the application of difference spectrophotometry.
Investigation of the difference absorption spectrum has been a common method for studying many aspects of hemoproteins such as cytochrome P450. The difference spectrophotometric technique, as applied in studies on binding of substrates to cytochrome P450, has resulted in certain mathematical artifacts which have been mistaken for real changes in binding equilibria. The purpose of this paper is to point out these artifacts.
Narasimhulu
S
S
Harrison Department of Surgical Research, University of Pennsylvania, Philadelphia 19104.
D001665:Binding Sites; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D008433:Mathematics; D008956:Models, Chemical; D013053:Spectrophotometry; D013379:Substrate Specificity
D003577:Cytochrome P-450 Enzyme System