Neuropeptides from the sinus gland of the lobster Homarus americanus: Characterization of hyperglycemic peptides.
In order to characterize hyperglycemic peptides from the sinus gland of the lobster, Homarus americanus, a bioassay was developed with juvenile H. gammarus. This assay was used for determining the hyperglycemic activity of peptides perified by reversed-phase high-performance liquid-chromatography, from acidic extracts of sinus gland. The major peptides are eluted in three sets of two peptides. Among them, two pairs show hyperglycemic activity when assayed on lobster; when assayed on crayfish, three peptides are active. The less hydrophobic pair consists of basic peptides (pI: 8.7), with a MW of 8633 Da., determined by fast-atom bombardment mass spectrometry. The most hydrophobic pair consists of acid peptides (pI: 5.0), with a MW of 8577 Da. Amino acid composition of the hyperglycemic peptides shows strong homologies within each pair.
Soyez
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Laboratoire de Neuroendocrinologie des Crustacés, URA 555 CNRS, Université Pierre et Marie Curie, Paris, France.
Noel
P Y
PY
Van Deijnen
J E
JE
Martin
M
M
Morel
A
A
Payen
G G
GG
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D060829:Arthropod Proteins; D003400:Astacoidea / Q000187:drug effects / Q000378:metabolism; D001681:Biological Assay; D001786:Blood Glucose / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005123:Eye; D007447:Invertebrate Hormones; D007526:Isoelectric Point; D013058:Mass Spectrometry; D008970:Molecular Weight; D008121:Nephropidae / Q000187:drug effects / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D009479:Neuropeptides / Q000302:isolation & purification / Q000494:pharmacology*
D000596:Amino Acids; D060829:Arthropod Proteins; D001786:Blood Glucose; D007447:Invertebrate Hormones; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; C015947:hyperglycemic hormone, crustacean