Inhibitory effects of protein kinase inhibitors and cytoskeletal inhibitors on Ca2(+)-free contraction of rat uterus.
In rat uterine smooth muscle, sustained Ca2(+)-free contraction was observed by oxytocin in Ca2(+)-free solution. This Ca2(+)-free contraction was effectively inhibited by protein kinase inhibitors and cytoskeletal inhibitors but myosin-light chain kinase (MLCK) inhibitors were not so effective. Simultaneous addition of a protein kinase inhibitor and a cytoskeletal inhibitor caused synergistic inhibition. These results suggest that the mechanism for Ca2(+)-free contraction involves some protein kinase and cytoskeletal elements rather than MLCK.
Karibe
H
H
Department of Molecular Pharmacology, Meiji College of Pharmacy, Tokyo, Japan.
Matsuo
K
K
Gokita
T
T
Uchida
M K
MK
D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000502:physiology*; D002384:Catalysis; D003571:Cytochalasin B / Q000494:pharmacology; D015638:Cytochalasin D / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D009219:Myosin-Light-Chain Kinase / Q000037:antagonists & inhibitors; D010052:Ovariectomy; D047428:Protein Kinase Inhibitors; D051381:Rats; D011919:Rats, Inbred Strains; D019311:Staurosporine; D014590:Uterine Contraction / Q000187:drug effects*
D000470:Alkaloids; D047428:Protein Kinase Inhibitors; D015638:Cytochalasin D; D003571:Cytochalasin B; D009219:Myosin-Light-Chain Kinase; D019311:Staurosporine; D002118:Calcium