title: Changes of several adrenal delta 4-steroids measured by HPLC-UV spectrometry in neonatal patients with congenital adrenal hyperplasia due to 21-hydroxylase deficiency.
abstract: We have developed an easy and rapid method of reverse-phase high-performance liquid chromatography (HPLC)-UV spectrometry for measuring adrenal delta 4-steroids. Three female neonates with adrenal 21-hydroxylase deficiency (2 salt-losers and 1 simple virilizer), two of whom were recalled by neonatal mass-screening for congenital adrenal hyperplasia (CAH), were diagnosed using this method. Changes of several adrenal steroids were examined in these patients before and after treatment with hydrocortisone. Before treatment, the cortisone and cortisol peaks were very low and those of 17 alpha-hydroxyprogesterone (17-OHP) and 21-deoxycortisol (21-DOF) were high in all 3 patients (17-OHP: 79.9-997 nmol/l, 21-DOF: 83.7-324 nmol/l). The androstenedione peak was also high in 2 of them. A peak produced by 21-deoxycortisone, which is a product of oxidation of 21-DOF at the C-11 position, was also detected in all cases (14.5-297 nmol/l). After treatment, all of these abnormally elevated delta 4-steroids decreased or disappeared. This new method is thought to be valuable for the rapid diagnosis of CAH, and especially for use in neonatal mass-screening for CAH.
authors.affiliation: Department of Pediatrics, Faculty of Medicine, Tokyo Medical and Dental University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Saisho
authors.forename: K
authors.initials: K
authors.lastname: Shimozawa
authors.forename: J
authors.initials: J
authors.lastname: Yata
mesh_terms: D015065:17-Hydroxycorticosteroids / Q000097:blood*; D019326:17-alpha-Hydroxyprogesterone; D000312:Adrenal Hyperplasia, Congenital / Q000209:etiology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003348:Cortisone / Q000031:analogs & derivatives / Q000097:blood; D003350:Cortodoxone / Q000097:blood*; D005260:Female; D006801:Humans; D006908:Hydroxyprogesterones / Q000097:blood*; D007231:Infant, Newborn; D008297:Male; D013250:Steroid Hydroxylases / Q000172:deficiency*; D013997:Time Factors
chemical_list: D015065:17-Hydroxycorticosteroids; D006908:Hydroxyprogesterones; C020882:21-deoxycortisone; C003556:21-deoxycortisol; D019326:17-alpha-Hydroxyprogesterone; D013250:Steroid Hydroxylases; D003348:Cortisone; D003350:Cortodoxone