The replication timing of the amplified dihydrofolate reductase genes in the Chinese hamster ovary cell line CHOC 400.
We have examined the timing of replication of the amplified dihydrofolate reductase genes in the methotrexate-resistant Chinese hamster ovary cell line CHOC 400 using two synchronization procedures. DNA replicated in the presence of 5-bromodeoxyuridine was collected from cells of various times during the DNA synthesis phase and the extent of replication for defined sequences was determined by Southern blotting analysis of CsCl density gradient fractions. We report that under these conditions the DHFR gene replicates throughout the course of S phase in a mode similar to the bulk of the replicated genomic DNA. This contrasts with previous data that shows the non-amplified DHFR gene replicates during the first quarter of S phase. Therefore, we conclude that gene amplification alters the replication timing of the DHFR gene in CHOC 400 cells.
Caddle
M S
MS
University of Vermont, College of Medicine, Department of Pathology, Burlington 05401.
Heintz
N H
NH
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004261:DNA Replication; D005784:Gene Amplification; D007399:Interphase; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*; D013997:Time Factors
D013762:Tetrahydrofolate Dehydrogenase