Oxidant stress leads to transcriptional activation of the human heme oxygenase gene in cultured skin fibroblasts.
Treatment of cultured human skin fibroblasts with near-UV radiation, hydrogen peroxide, and sodium arsenite induces accumulation of heme oxygenase mRNA and protein. In this study, these treatments led to a dramatic increase in the rate of RNA transcription from the heme oxygenase gene but had no effect on mRNA stability. Transcriptional activation, therefore, appears to be the major mechanism of stimulation of expression of this gene by either oxidative stress or sulfydryl reagents.
Keyse
S M
SM
Swiss Institute for Experimental Cancer Research, Epalinges, Switzerland.
Applegate
L A
LA
Tromvoukis
Y
Y
Tyrrell
R M
RM
D000199:Actins / Q000235:genetics; D001151:Arsenic / Q000494:pharmacology*; D018053:Arsenites; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects; D015971:Gene Expression Regulation, Enzymologic; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007700:Kinetics; D006899:Mixed Function Oxygenases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012867:Skin / Q000201:enzymology*; D017670:Sodium Compounds; D014158:Transcription, Genetic / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays
D000199:Actins; D018053:Arsenites; D012333:RNA, Messenger; D017670:Sodium Compounds; C017947:sodium arsenite; D006861:Hydrogen Peroxide; D006899:Mixed Function Oxygenases; D006419:Heme Oxygenase (Decyclizing); D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D001151:Arsenic