Complete amino acid sequence of arachin subunit of molecular weight 21,000.
A subunit of molecular weight 21,000 from arachin, the major peanut protein, was isolated in pure form and primary structure was determined. The subunit was fragmented with CNBr, trypsin, and NBS; the fragments were separated and isolated by PAGE, gel filtration, Dowex treatment, and paper electrophoresis, and Edman degradation on each fragment, including the intact subunit, was performed. The PTH-amino acids thus obtained were identified by UV spectroscopy and TLC. The complete sequence of 176 residues was established by overlapping technique.
Bhushan
R
R
Dept. of Chemistry, University of Roorkee, India.
Reddy
K R
KR
D000595:Amino Acid Sequence; D010367:Arachis / Q000032:analysis; D001974:Bromosuccinimide; D003488:Cyanogen Bromide; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments; D010940:Plant Proteins / Q000302:isolation & purification; D014357:Trypsin
D010446:Peptide Fragments; D010940:Plant Proteins; C000102:Arachin; D014357:Trypsin; D001974:Bromosuccinimide; D003488:Cyanogen Bromide