title: ADH, renal, and circulatory responses to adrenergic stimulation in anterior third ventricle.
abstract: The anterodorsal part of the third ventricle of conscious ducks was perfused intracerebroventricularly (icv) for 10 min with norepinephrine (NE) or with its agonists phenylephrine (alpha 1, Phe), isoproterenol (beta, Iso), and clonidine (alpha 2, Clo) in artificial CSF (aCSF). Their effects on the plasma level of antidiuretic hormone (AVT, arginine vasotocin in birds), urine excretion, heart rate (HR), and mean arterial pressure (MAP) were investigated in steady-state water diuresis. The correct position of the icv cannula was confirmed by enhanced AVT release and antidiuresis in response to icv perfusion of aCSF made hypertonic (400 mosmol/kgH2O) by adding NaCl. Icv perfusion with hypertonic aCSF and 750 ng/min NE had comparable effects on AVT release and urine excretion, but hypertonic aCSF caused small increases in MAP and HR, whereas NE depressed both MAP and HR. Antidiuresis and circulatory depression caused by NE icv perfusion was dose dependent. Among the adrenergic agonists perfused at similar doses (188 ng/min), only Iso stimulated AVT release. Iso and Phe had small depressive effects on MAP and HR (less than 10%). Clo depressed circulation by greater than 20% for longer than 60 min, and AVT release became significantly reduced 30 min after the start of icv perfusion. The consistent results in ducks contrast with the equivocal data hitherto reported for central stimulations with NE or its agonists in mammals and may be due to the concentric perfusion system used in our study for localized stimulations in the vicinity of the paraventricular nucleus.
authors.affiliation: Max-Planck-Institut für physiologische und klinische Forschung, Bad Nauheim, Federal Republic of Germany.
authors.forename: C
authors.initials: C
authors.lastname: Simon-Oppermann
authors.forename: O
authors.initials: O
authors.lastname: Günther
mesh_terms: D000818:Animals; D001775:Blood Circulation / Q000187:drug effects; D002552:Cerebral Ventricles / Q000502:physiology*; D004231:Diuresis / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004372:Ducks; D005260:Female; D007276:Injections, Intraventricular; D007668:Kidney / Q000378:metabolism*; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D010477:Perfusion; D012462:Saline Solution, Hypertonic / Q000494:pharmacology; D013566:Sympathomimetics / Q000494:pharmacology*; D014668:Vasotocin / Q000097:blood*
chemical_list: D012462:Saline Solution, Hypertonic; D013566:Sympathomimetics; D014668:Vasotocin; D009638:Norepinephrine