Cloning and expression of a cDNA encoding a novel human neurotrophic factor.
A cDNA encoding a novel human neurotrophic factor (designated nerve growth factor-2; NGF-2) was cloned from a human glioma cDNA library using a synthetic DNA corresponding to human nerve growth factor (NGF). The cloned cDNA encodes a polypeptide composed of 257 amino acid residues including a prepro-sequence of 138 residues and a mature region of 119 residues. The amino acid sequence of human NGF-2 exhibits 58% similarity with that of human NGF. Conditioned medium of COS-7 cells transfected with an expression plasmid for human NGF-2 cDNA supported the survival of sensory neurons isolated from dorsal root ganglia of embryonic chicks. A 1.5 kb of NGF-2 mRNA can be detected from an early development stage in rat brain, by Northern blotting analysis.
Kaisho
Y
Y
Biotechnology Research Laboratories, Takeda Chemical Industries Ltd, Osaka, Japan.
Yoshimura
K
K
Nakahama
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001681:Biological Assay; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D005910:Glioma; D006801:Humans; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000235:genetics*; D051381:Rats; D015183:Restriction Mapping
D009414:Nerve Growth Factors; D004247:DNA