High activity neurons in the reticular formation of the medulla oblongata: a high-resolution autoradiographic 2-deoxyglucose study.
Accumulation of radioactivity in the medulla oblongata of the rat was assessed with high resolution autoradiography after injection of [3H]2-deoxyglucose or [14C]2-deoxyglucose. The autoradiographic approach employed allowed analysis at the cellular level. A salient feature of autoradiograms was the presence of foci of very high silver grain density in the reticular formation. These heavily labeled foci were shown to be associated with neurons by combined Acridine Orange staining and autoradiography. The high activity neurons in the ventral lateral medulla were predominantly located in the caudal portion of the paragigantocellular reticular nucleus. In addition, a group of high activity neurons was present in the dorsal reticular formation. The potential involvement of the high activity neurons in cardiovascular and respiratory regulation is discussed. In dry-mount autoradiograms produced after injection of [3H]2-deoxyglucose, certain small cells, presumably glial cells, were observed throughout the brainstem to accumulate radioactivity to a greater extent than the surrounding tissue. High activity neurons and high activity glial cells were observed for 5 min and 45 min survival intervals after intravenous injection of [3H]2-deoxyglucose. The similar appearance of autoradiograms at 5 min and 45 min after injection of [3H]2-deoxyglucose indicates that the utility of a 5-min survival period deserves further evaluation for assessment of functional activity patterns in brain.
Duncan
G E
GE
Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill 27599.
Kaldas
R G
RG
Mitra
K E
KE
Breese
G R
GR
Stumpf
W E
WE
D000818:Animals; D001345:Autoradiography / Q000379:methods; D001933:Brain Stem / Q000033:anatomy & histology / Q000502:physiology; D002250:Carbon Radioisotopes; D003837:Deoxy Sugars / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008526:Medulla Oblongata / Q000033:anatomy & histology / Q000378:metabolism / Q000502:physiology*; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D012154:Reticular Formation / Q000033:anatomy & histology / Q000378:metabolism / Q000502:physiology*; D013997:Time Factors; D014316:Tritium
D002250:Carbon Radioisotopes; D003837:Deoxy Sugars; D014316:Tritium; D003847:Deoxyglucose