title: Effect of calcium citrate-malate on skeletal development in young, growing rats.
abstract: It has been previously demonstrated that calcium from calcium citrate-malate (CCM), a mixture of calcium, citric acid and malic acid, is better-absorbed than calcium from calcium carbonate (CaCO3) in humans and in rats. It was of interest to determine if this differential in absorption would result in differences in bone development under chronic feeding conditions. The present study was designed to compare CCM with CaCO3 for effects on bone development in weanling female C/D rats fed either CCM or CaCO3 at 0.3 or 0.6% dietary Ca for 4 or 12 wk. There was a nonsignificant trend for rats fed CCM to weigh more and have larger bones than rats fed CaCO3. Histologic evaluation of cortical and trabecular bone revealed normal bone formation in all rats. Trabecular bone was significantly affected by calcium level and source. The 0.3% Ca diets (either source) resulted in reduced trabecular bone volumes in tibias. After 4 wk, rats fed CCM had 23-25% more trabecular bone than rats fed CaCO3. By 12 wk, the difference was even greater; rats fed CCM had 44-47% more trabecular bone than rats fed CaCO3. Dietary calcium source did not affect cortical bone. It is concluded that because of its positive effects on bone, CCM is a more bioavailable calcium source than CaCO3.
authors.affiliation: Procter & Gamble Company, Miami Valley Laboratories, Cincinnati, OH 45239.
authors.forename: B A
authors.initials: BA
authors.lastname: Kochanowski
mesh_terms: D000818:Animals; D001682:Biological Availability; D001846:Bone Development / Q000187:drug effects*; D001842:Bone and Bones / Q000033:anatomy & histology; D002118:Calcium / Q000008:administration & dosage* / Q000493:pharmacokinetics; D002119:Calcium Carbonate / Q000494:pharmacology; D002951:Citrates / Q000494:pharmacology*; D019343:Citric Acid; D004032:Diet; D005260:Female; D008293:Malates / Q000494:pharmacology*; D051381:Rats
chemical_list: D002951:Citrates; D008293:Malates; D019343:Citric Acid; C030298:malic acid; D002119:Calcium Carbonate; D002118:Calcium