title: The effects of retina-derived factors on the electrical and ultrastructural properties of the bovine pigment epithelium.
abstract: The transepithelial potential (TEP) and short circuit current (SCC) of the bovine retina pigment epithelium (RPE) were monitored throughout a 3hr period. Application of retina-derived factors to the apical side of the preparation resulted in a stabilization of the TEP and SCC, followed by a secondary rise in both electrical parameters. The late, secondary rise could be eliminated or reduced by the administration of cycloheximide suggesting a dependence upon protein synthesis. The active component(s) is not heat labile and has an apparent molecular weight between 1,000-10,000. After 3 hr of incubation, both control and factor-treated RPE had normal appearing nuclei and mitochondria; however, the factor-treated preparations were significantly thicker (apical to basal membrane) and had more apical processes than the control tissues.
authors.affiliation: Department of Physiology, Colorado State University, Fort Collins 80523.
authors.forename: E L
authors.initials: EL
authors.lastname: Pautler
authors.forename: D
authors.initials: D
authors.lastname: Beezley
mesh_terms: D000818:Animals; D001685:Biological Factors / Q000502:physiology*; D002417:Cattle; D046508:Culture Techniques; D003513:Cycloheximide / Q000494:pharmacology; D004594:Electrophysiology; D005346:Fibroblast Growth Factors; D006133:Growth Substances / Q000502:physiology; D010857:Pigment Epithelium of Eye / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D012160:Retina / Q000502:physiology*; D014020:Tissue Extracts / Q000502:physiology
chemical_list: D001685:Biological Factors; D006133:Growth Substances; D014020:Tissue Extracts; C039825:eye-derived growth factor; D005346:Fibroblast Growth Factors; D003513:Cycloheximide