Similarities between metallothionein and low molecular weight testicular cadmium-binding protein.
The incorporation of 35S-cysteine and 3H-glutamic acid was studied in mouse hepatic and renal metallothionein and in testicular cadmium-binding protein of similar molecular weight. Preferential incorporation of 35S-cysteine over 3H-glutamic acid was observed not only in hepatic and renal metallothionein, but also in testicular cadmium-binding protein. When the antigenic reactivity of these proteins was compared, all three proteins reacted with the metallothionein antibody. These similarities suggest that the low molecular weight testicular cadmium-binding protein is apparently metallothionein.
Shaikh
Z A
ZA
Department of Pharmacology and Toxicology, University of Rhode Island, Kingston 02881.
Tewari
P C
PC
D000818:Animals; D002104:Cadmium / Q000378:metabolism; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis; D008297:Male; D008668:Metallothionein / Q000032:analysis*; D051379:Mice; D008811:Mice, Inbred DBA; D008970:Molecular Weight; D011863:Radioimmunoassay; D013737:Testis / Q000032:analysis*
C004203:cadmium-binding protein; D002104:Cadmium; D008668:Metallothionein