Lymphocyte response to IgE regulatory factors in allergic patients during the course of immunotherapy.
In previous studies we demonstrated isotype-specific but antigen non-restricted soluble factors produced by human lymphocytes of allergic patients and normal controls. In a subsequent investigation we isolated these soluble factors from allergic patients classified in various groups according to their immunotherapy (IT) time periods and from controls, and we tested them on lymphocytes of healthy controls. Then, we decided to amplify the study to include the effects on allergic patients' lymphocytes. Bidirectional mixed cultures were grown with lymphocytes from healthy controls and allergic patients with different IT time periods and supernatants obtained from chromatography were tested on lymphocytes of twenty-one allergic patients. None of the enhancing factors showed statistical significant effects on IgE synthesis of allergic lymphocytes. There clearly existed a change in the production of suppressor factors during the course of IT, but the long time period of IT did not increase this production.
Tokushima
M
M
Departamento de Alergología, Facultad de Medicina, Universidad de Navarra, Pamplona, Spain.
Sanz
M L
ML
de las Marinas
M D
MD
Oehling
A
A
D001402:B-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D003888:Desensitization, Immunologic; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis* / Q000494:pharmacology*; D008222:Lymphokines / Q000494:pharmacology*; D029584:Prostatic Secretory Proteins; D012130:Respiratory Hypersensitivity / Q000276:immunology* / Q000473:pathology / Q000628:therapy; D013491:Suppressor Factors, Immunologic / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000378:metabolism
C037161:IGE class-specific suppressor factor; D008222:Lymphokines; D029584:Prostatic Secretory Proteins; D013491:Suppressor Factors, Immunologic; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors; D007073:Immunoglobulin E