title: The primary structure of lupin seed 5.8 S ribosomal RNA.
abstract: The lack of colinearity between nucleotide sequence of the lupin 5.8 S rDNA gene (Rafalski, A.J., Wiewiórowski, M. and Soll, D. (1983) FEBS Lett. 152, 241-246) and 5.8 S rRNA of other plants (Erdmann, V.A. and Wolters, J. (1986) Nucleic Acids Res. 14, r1-r59.) prompted us to clarify this point by sequencing the native lupin 5.8 S rRNA. The sequence analysis was carried out using enzymatic and chemical methods. Lupin seed 5.8 S rRNA contains 164 nucleotides, including four modified ones: two residues of 2'-O-methylguanosine, one pseudouridine and one 2'-O-methyladenosine. The nucleotide sequence homology with the other plant 5.8 S rRNAs is approx. 88-96%.
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań.
authors.forename: M Z
authors.initials: MZ
authors.lastname: Barciszewska
authors.forename: T D
authors.initials: TD
authors.lastname: Mashkova
authors.forename: J
authors.initials: J
authors.lastname: Barciszewski
mesh_terms: D001483:Base Sequence; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010944:Plants / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D012335:RNA, Ribosomal; D012340:RNA, Ribosomal, 5.8S