Expression of HIV-1 gp120 and human soluble CD4 by recombinant baculoviruses and their interaction in vitro.
The soluble domains of the envelope glycoprotein of HIV-1 (gp120) and human CD4 (sCD4) have been individually expressed in insect cells using recombinant baculoviruses. Each product is secreted from infected cells and accumulates in the surrounding media to levels of 1-2 mg/liter of 2 x 10(9) cells. Both molecules have full biological activity, and conditioned media from infected cells have been used to establish a simple assay for gp120-sCD4 interaction that is highly specific and amenable to mass screening. The crystallization of sCD4 purified from this source is reported.
Morikawa
Y
Y
NERC Institute of Virology, Oxford, England.
Overton
H A
HA
Moore
J P
JP
Wilkinson
A J
AJ
Brady
R L
RL
Lewis
S J
SJ
Jones
I M
IM
D000818:Animals; D001483:Base Sequence; D015704:CD4 Antigens / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D003001:Cloning, Molecular; D003460:Crystallization; D015342:DNA Probes; D015699:HIV Envelope Protein gp120 / Q000096:biosynthesis* / Q000235:genetics; D007304:Insect Viruses / Q000235:genetics*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
D015704:CD4 Antigens; D015342:DNA Probes; D015699:HIV Envelope Protein gp120; D011994:Recombinant Proteins