[Comparative study of diffusion coefficients of alpha-lactalbumins and lysozyme using polarization interferometer].
The method of macroscopic diffusion with polarization optics has been applied to compare the translational diffusion coefficients for three proteins with similar three-dimensional structures in different conformational states. It has been shown that the values of diffusion coefficients obtained by the method are almost the same as those obtained by quasielastic light scattering. However this method is more available since it is less sensitive to aggregation and requires less amount of protein for investigation.
Bychkova
V E
VE
Bartoshevich
S F
SF
Klenin
S I
SI
D000818:Animals; D002417:Cattle; D004058:Diffusion; D006801:Humans; D007368:Interferometry; D007768:Lactalbumin / Q000032:analysis*; D008433:Mathematics; D009113:Muramidase / Q000032:analysis*; D011487:Protein Conformation
D007768:Lactalbumin; D009113:Muramidase
Sravnitel'noe issledovanie koéffitsientov diffuzii alpha-laktal'buminov i lizotsima s pomoshch'iu poliarizatsionnogo interferometra.