On the hydrodynamic instability of hemoglobin solutions.
The formation of heterogeneous fibers well observable under a magnifying glass in stirred "stroma-free" hemolysates of outdated banked erythrocytes was found to be predominantly due to random surface denaturation of both hemoglobin and non-heme proteins at the air-liquid interface. Electrophoretic analysis revealed that the precipitated and washed fibers contain residual membrane proteins as well as a certain amount of hemoglobin. The latter, however, largely remains in solution.
Pristoupil
T I
TI
Institute of Hematology and Blood Transfusion, Prague, Czechoslovakia.
Marík
T
T
D001802:Blood Substitutes; D011232:Chemical Precipitation; D004355:Drug Stability; D004591:Electrophoresis, Polyacrylamide Gel; D006454:Hemoglobins; D006801:Humans; D066298:In Vitro Techniques; D010477:Perfusion; D011489:Protein Denaturation; D012996:Solutions
D001802:Blood Substitutes; D006454:Hemoglobins; D012996:Solutions