Electron microscopy of native and artificial methylreductase high-molecular-weight complexes in strain Gö 1 and Methanococcus voltae.
The preparation of inside-out vesicles from methanogenic bacteria with protein cell walls was improved with regard to the preservation of structure and localization of membrane-bound proteins. Complexes similar to the methanoreductosome in the methanogenic bacterium Gö 1 were also found attached to the inner aspect of the cytoplasmic membrane of Methanococcus voltae. Methanoreductosomes were purified from crude extracts of Gö 1-cells by affinity chromatography. Under specific conditions at high protein concentrations methyl-CoM-methylreductase molecules isolated from Gö 1-cells could be reassociated to spherical complexes of various sizes, with an appearance similar to that of methanoreductosomes isolated from strain Gö 1.
Hoppert
M
M
Institut für Mikrobiologie, Göttingen, FRG.
Mayer
F
F
D019605:Euryarchaeota / Q000201:enzymology* / Q000648:ultrastructure; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000032:analysis*; D010088:Oxidoreductases / Q000032:analysis*; D011523:Protoplasts / Q000201:enzymology / Q000648:ultrastructure
D009097:Multienzyme Complexes; D010088:Oxidoreductases; C023895:methyl coenzyme M methylreductase