Comparison of non-collagenous type IV collagen subunits in human glomerular basement membrane, alveolar basement membrane, and placenta.
This study examines the similarities and differences in the noncollagenous domain (NC1) of type IV collagen from human glomerular basement membrane (hGBM), alveolar basement membrane (hABM), and placenta (hPBM). Following collagenase digestion, NC1 domain was isolated on Bio-Gel A-0.5m or by cation exchange chromatography on S-Sepharose. NC1 from each source was characterized by SDS PAGE, and two dimension NEPHGE/SDS PAGE. Immunoblotting and ELISA inhibition was performed using antibody probes specific for M28 , M28+, M26 and M24 monomer subunits of human NC1. It was observed that all NC1 subunits were present in hGBM and hABM derived material, however M28 and M28+ monomers were absent in hPBM NC1. These findings indicate that while alpha 1(IV) and alpha 2(IV) collagen chains are present in hGBM, hABM and hPBM, alpha 3(IV) and alpha 4(IV) collagen chains are only found in hGBM and hABM but are absent in hPBM. It can now be appreciated that heterogeneity of alpha (IV) chain composition exists in basement membranes from various organs.
Shen
G Q
GQ
Department of Pediatrics, University of Minnesota, Minneapolis 55455.
Butkowski
R
R
Cheng
T
T
Wieslander
J
J
Katz
A
A
Cass
J
J
Fish
A J
AJ
D001485:Basement Membrane / Q000032:analysis; D003094:Collagen / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D007678:Kidney Glomerulus / Q000032:analysis; D010920:Placenta / Q000032:analysis; D011247:Pregnancy; D011487:Protein Conformation; D011650:Pulmonary Alveoli / Q000032:analysis; D014018:Tissue Distribution
D003094:Collagen