title: Quantitative analysis of the effect of freezing on donor sperm motion kinetics.
abstract: One hundred ninety-two semen specimens from 14 donors were analyzed on a CellSoft Semen Analyzer (CRYO Resources, New York, NY) before and after freezing. Mean post-thaw motility decreased by 52%. Correlation of the percent decrease in post-thaw versus prefreeze motility was significant but of poor predictive value. Comparisons of the percent change in post-thaw motility and sperm motion kinetics between four discrete ranges of prefreeze motility (32% to 66%, 67% to 76%, 77% to 84%, 85% to 94%) revealed that the effect of freezing on sperm cell survival was equivalent between all ranges. However, significant differences occurred between these ranges for curvilinear velocity, straight line velocity, and mean amplitude of lateral head displacement but not for linearity nor beat cross frequency. All correlations between prefreeze and post-thaw motion variables were significant but closest for curvilinear velocity and straight line velocity. Furthermore, correlations of prefreeze versus post-thaw velocity measurements were strongest for those cells within the 65% to 85% range of prefreeze motility. We suggest that sperm survival is independent of prefreeze motility. However, velocity kinetics appear stable after freezing for those specimens that had an initial motility of 65% to 85%.
authors.affiliation: Department of Obstetrics, Gynecology, and Reproductive Sciences, University of Manitoba, Winnipeg, Canada.
authors.forename: P A
authors.initials: PA
authors.lastname: Fernandes
authors.forename: J A
authors.initials: JA
authors.lastname: McCoshen
authors.forename: M
authors.initials: M
authors.lastname: Cheang
authors.forename: J V
authors.initials: JV
authors.lastname: Kredentser
authors.forename: A M
authors.initials: AM
authors.lastname: Wodzicki
mesh_terms: D000704:Analysis of Variance; D005615:Freezing; D006801:Humans; D007700:Kinetics; D008297:Male; D013081:Sperm Motility