Characterization of filamentous bacteriophage phi Lf from Xanthomonas campestris pv. campestris.
A filamentous phage, phi Lf, which specifically infects Xanthomonas campestris pv. campestris was isolated. The phage particle measured 1,000 (+/- 200) x 8 nm. It formed turbid plaques of about 1 mm in diameter. During multiplication, the progeny virions extruded into the medium without retarding host cell growth. Stocks were stable for 6 months at 4 degrees C and survived treatment at 80 degrees C for 10 min. Treatment with chloroform, ethanol or acetone completely destroyed infectivity; ethyl ether and methanol inactivated 98 to 99% of the phage. SDS-polyacrylamide gel electrophoresis showed a major coat protein band of approximate Mr 4000 whereas an immunoprecipitation test detected the existence of two coat protein species. The phage genome was shown to be a single-stranded DNA molecule. A physical map was constructed and the DNA size was calculated to be 5.9 kb. Cells treated with Tris-HCl containing CaCl2 and polyethylene glycol 6000 were transfected by replicative form DNA at a frequency of 3.4 x 10(3) p.f.u./micrograms.
Tseng
Y H
YH
Department of Botany, National Chung Hsing University, Taichung, Taiwan, Republic of China.
Lo
M C
MC
Lin
K C
KC
Pan
C C
CC
Chang
R Y
RY
D001435:Bacteriophages / Q000032:analysis* / Q000302:isolation & purification / Q000502:physiology / Q000648:ultrastructure; D002213:Capsid / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D008854:Microscopy, Electron; D011233:Precipitin Tests; D015183:Restriction Mapping; D014974:Xanthomonas
D004279:DNA, Viral