Concanavalin A affects glycosaminoglycans cellular and extracellular accumulation in cultured embryonic fibroblasts.
Incorporation of 3H glucosamine and 35SO4 into glycosaminoglycans and proteoglycans produced and secreted by 7, 11 and 14 day chick embryo fibroblasts in vitro after concanavalin A treatment has been determined. Lectin differently affects 3H and 35SO4 incorporation. It enhances 3H labelled GAG accumulation in both cellular and extracellular compartments. Total incorporation of 35SO4 remains unchanged whereas the intracellular one is stimulated and the extracellular is reduced. All the effects are more relevant in the early stages of development. HA and PG cellular and extracellular accumulation seems to be independently regulated.
Locci
P
P
Dipartimento di Medicina sperimentale, Università di Perugia, Italy.
Evangelisti
R
R
Lilli
C
C
Becchetti
E
E
Carinci
P
P
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D003208:Concanavalin A / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism; D013462:Sulfur Radioisotopes; D014316:Tritium / Q000378:metabolism
D006025:Glycosaminoglycans; D011509:Proteoglycans; D013462:Sulfur Radioisotopes; D014316:Tritium; D003208:Concanavalin A