[The determination of trace amounts of protein in solutions containing surface-active substances].
A rapid and sensitive method for protein determination (0.5-16 micrograms) in samples of any volume containing various surfactants in concentration up to 1% is suggested. The method includes the protein acid denaturation, the solution of acid insoluble precipitate of detergent in ethanol (25-30%), the protein determination on nitrocellulose filter, dyeing by aminoblack 10 B, elution of dyed complex and colorimetric determination at 630 nm.
Sirota
N P
NP
Sirota
T V
TV
D000580:Amido Black; D002138:Calibration; D004305:Dose-Response Relationship, Drug; D005374:Filtration / Q000295:instrumentation / Q000379:methods; D011489:Protein Denaturation / Q000187:drug effects; D011506:Proteins / Q000032:analysis*; D012996:Solutions; D013501:Surface-Active Agents / Q000032:analysis*
D011506:Proteins; D012996:Solutions; D013501:Surface-Active Agents; D000580:Amido Black
Opredelenie mikrokolichestv belka v rastvorakh, soderzhashchikh poverkhnostno-aktivnye veshchestva.