title: An improved method of analysing 2,5-hexanedione in urine.
abstract: A short gas-chromatographic method for analysing urinary concentrations of 2,5-hexanedione is based on acid hydrolysis of urine at pH below 0.1 and "purification" of the urine samples by microcolumns containing an octadecyl-silane phase. A 5% acetonitrile solution allows a fairly selective elution of 2,5-hexanedione from the microcolumns. Recovery of 2,5-hexanedione from urine is as great as 79.9%. The variation coefficient of the measurements is 2.8%. The results obtained from different working conditions and using packed or wide bore or capillary gas-chromatographic columns are reported.
authors.affiliation: Institute of Occupation Medicine, University of Verona, Policlinico Borgo Roma, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Perbellini
authors.forename: D M
authors.initials: DM
authors.lastname: Marhuenda Amoros
authors.forename: A C
authors.initials: AC
authors.lastname: Cardona Llorens
authors.forename: C
authors.initials: C
authors.lastname: Giuliari
authors.forename: F
authors.initials: F
authors.lastname: Brugnone
mesh_terms: D002138:Calibration; D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods*; D006588:Hexanones / Q000652:urine*; D006801:Humans; D007659:Ketones / Q000652:urine*
chemical_list: D006588:Hexanones; D007659:Ketones; C011269:2,5-hexanedione