[Antigenicity study of buspirone hydrochloride in guinea pigs and mice].
Buspirone hydrochloride(buspirone) and buspirone-ovalbumin mixture were examined for their antigenicity in guinea pigs and mice in comparison with ovalbumin (OVA) and 2, 4-dinitrochlorobenzene (DNCB)-OVA conjugate. The results obtained were as follows: 1. When guinea pigs were sensitized with buspirone or buspirone-OVA emulsified with Freund's complete adjuvant (FCA), these animals showed negative reactions in active systemic anaphylaxis (ASA), active cutaneous anaphylaxis (ACA), passive cutaneous anaphylaxis (PCA), passive hemagglutination (PHA) and Schultz-Dale test. 2. When mice were sensitized with buspirone or buspirone-OVA adsorbed to alum, these animals revealed a negative reaction in PCA using rats. 3. As positive controls, guinea pigs were sensitized with OVA or DNCB-OVA emulsified with FCA, and mice with OVA or DNCB-OVA adsorbed to alum. As a result, these animals disclosed positive reactions in ASA, ACA, PCA, PHA and Schultz-Dale test. As shown above, buspirone was considered to possess neither antigenic nor haptenic properties.
Kawano
S
S
Drug Safety Research Department, Bristol-Myers Research Institute, Ltd., Aichi, Japan.
Kohmura
H
H
Ohta
S
S
Takahashi
N
N
D000707:Anaphylaxis / Q000139:chemically induced; D000818:Animals; D000941:Antigens / Q000276:immunology*; D002065:Buspirone / Q000276:immunology*; D004137:Dinitrochlorobenzene; D006168:Guinea Pigs; D006384:Hemagglutination / Q000187:drug effects; D007428:Intradermal Tests; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D010047:Ovalbumin / Q000276:immunology; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects
D000941:Antigens; D004137:Dinitrochlorobenzene; D010047:Ovalbumin; D002065:Buspirone