High-performance liquid chromatographic analysis of lung phospholipids and their precursors in the offspring of diabetic rats.
Pulmonary phospholipids and their precursors and metabolites were assayed in the offspring of streptozotocin-induced diabetic rats at 19 and 21 days gestation and at 2 days after birth by two unique methods that employ high performance liquid chromatography combined with automated phosphorus analysis. In general, lung phospholipids were not different between offspring of control versus diabetic mothers. Levels of phosphatidylglycerol, however, were decreased in the offspring of diabetics. Lysophosphatidylcholine appears to be increased in the lungs of offspring of diabetic mothers, suggesting that maternal diabetes is associated with alterations in the remodeling of phosphatidylcholine.
Boehme
D S
DS
Department of Pharmacology and Nutrition, University of Southern California School of Medicine, Los Angeles 90033.
Roberts
C M
CM
Bessman
S P
SP
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005260:Female; D005333:Fetus / Q000378:metabolism; D005865:Gestational Age; D008168:Lung / Q000032:analysis* / Q000378:metabolism; D008244:Lysophosphatidylcholines / Q000378:metabolism; D010715:Phosphatidylglycerols / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis* / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains
D008244:Lysophosphatidylcholines; D010715:Phosphatidylglycerols; D010743:Phospholipids