title: Isolation and structure of a novel charged member of the red-pigment-concentrating hormone-adipokinetic hormone family of peptides isolated from the corpora cardiaca of the blowfly Phormia terraenovae (Diptera).
abstract: A hypertrehalosaemic neuropeptide from the corpora cardiaca of the blowfly Phormia terraenovae has been isolated by reversed-phase h.p.l.c., and its primary structure was determined by pulsed-liquid phase sequencing employing Edman chemistry after enzymically deblocking the N-terminal pyroglutamate residue. The C-terminus was also blocked, as indicated by the lack of digestion when the peptide was incubated with carboxypeptidase A. The octapeptide has the sequence pGlu-Leu-Thr-Phe-Ser-Pro-Asp-Trp-NH2 and is clearly defined as a novel member of the RPCH/AKH (red-pigment-concentrating hormone/adipokinetic hormone) family of peptides. It is the first charged member of this family to be found. The synthetic peptide causes an increase in the haemolymph carbohydrate concentration in a dose-dependent fashion in blowflies and therefore is named 'Phormia terraenovae hypertrehalosaemic hormone' (Pht-HrTH). In addition, receptors in the fat-body of the American cockroach (Periplaneta americana) recognize the peptide, resulting in carbohydrate elevation in the blood. However, fat-body receptors of the migratory locust (Locusta migratoria) do not recognize this charged molecule, and thus no lipid mobilization is observed in this species.
authors.affiliation: Institut für Zoologie, Heinrich-Heine-Universität, Düsseldorf, Federal Republic of Germany.
authors.forename: G
authors.initials: G
authors.lastname: Gäde
authors.forename: H
authors.initials: H
authors.lastname: Wilps
authors.forename: R
authors.initials: R
authors.lastname: Kellner
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004175:Diptera / Q000032:analysis*; D007301:Insect Hormones / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009490:Neurosecretory Systems / Q000032:analysis*; D009842:Oligopeptides / Q000302:isolation & purification* / Q000378:metabolism*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives
chemical_list: D000596:Amino Acids; D007301:Insect Hormones; D009842:Oligopeptides; C044833:adipokinetic hormone; C065368:DAKH peptide; C022059:red pigment-concentrating hormone; D011761:Pyrrolidonecarboxylic Acid