Immunohistochemical methods for the visualization of Mycobacterium paratuberculosis in bovine tissues.
The peroxidase-antiperoxidase (PAP), streptavidin-biotin (SB), and avidin-biotin-complex (ABC) techniques have been evaluated for the visualization of Mycobacterium paratuberculosis (Mp) in formalin-fixed, paraffin-embedded bovine tissues. The used immunoperoxidase techniques were comparatively better than the Ziehl-Neelsen stain, specially for the demonstration of small number of mycobacteria in tissue sections.
Massone
A R
AR
Institute of Pathology, Faculty of Veterinary Sciences, La Plata, Argentina.
Martín
A A
AA
Ibargoyen
G S
GS
Gimeno
E J
EJ
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D005260:Female; D007150:Immunohistochemistry; D007422:Intestines / Q000382:microbiology; D008198:Lymph Nodes / Q000382:microbiology; D009161:Mycobacterium / Q000302:isolation & purification*; D010283:Paratuberculosis / Q000382:microbiology*; D012189:Retrospective Studies