Deletions in the cytoplasmic domain of the polymeric immunoglobulin receptor differentially affect endocytotic rate and postendocytotic traffic.
We have examined the function of the cytoplasmic domain of the polymeric immunoglobulin receptor (pIg-R) by producing two separate deletions in the cytoplasmic domain of the pIg-R, expressing the mutant receptors in polarized MDCK cells, and analyzing each for their effects on receptor and ligand traffic. Deletion of the C-terminal 30 amino acids (726-755) reduces the rate of internalization of receptor-bound ligand from the basolateral surface. However, this mutation has no effect on delivery of receptor from the Golgi to the basolateral surface or the post-endocytotic traffic of receptor and ligand. Mutation of a tyrosine at position 734 to serine produces a receptor with a similar phenotype. If residues 670-707 are deleted from the middle of the cytoplasmic domain, both basolateral delivery and internalization are unaffected. However, unlike wild type, after endocytosis from the basolateral surface, both receptor and ligand are largely degraded. We reported previously that deletion of the entire cytoplasmic domain prevents the basolateral delivery of newly synthesized receptor (Mostov, K.E., de Bruyn Kops, A., and Deitcher, D.L. (1986) Cell 47, 359-364). In contrast, the mutants reported here are delivered to the basolateral surface, suggesting that only residues 653-669 and/or 708-725 are necessary for basolateral delivery. Thus, different deletions in the cytoplasmic domain of the pIg-R can produce mutant receptors which alter different aspects of receptor traffic.
Breitfeld
P P
PP
Department of Pediatrics (Hematology), University of Massachusetts Medical School, Worcester 01655.
Casanova
J E
JE
McKinnon
W C
WC
Mostov
K E
KE
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000276:immunology; D004274:DNA, Recombinant / Q000378:metabolism; D004705:Endocytosis; D007140:Immunoglobulin Fab Fragments / Q000378:metabolism; D007700:Kinetics; D009154:Mutation; D011971:Receptors, Immunologic; D012635:Secretory Component / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism
D004274:DNA, Recombinant; D007140:Immunoglobulin Fab Fragments; D011971:Receptors, Immunologic; D012635:Secretory Component