title: Ligandin binding phthalein complexone complex of technetium for hepatic function studies.
abstract: In our pursuit for liver functional diagnosis, development of bifunctional radiopharmaceutical containing iminodiacetic acid (IDA), as the technetium chelating site along with phthalein or fluorescein structure, the skeleton of BSP and Rose Bengal, long used for the assessment of liver function is considered. Among the various PC, PPC, TPC and calcein IDA derivatives commercially available, 99mTc-PC (PC: 3,3'-bis(N, N-dicarboxymethylaminomethyl)o-cresolphthalein) showed the highest hepatobiliary excretion. The functionality of the various technetium labeled phthalein and fluorescein IDA derivatives was evaluated by competitive BSP binding studies and by comparative binding with the hepatocyte specific protein, ligandin.
authors.affiliation: Department of Radiopharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, School of Medicine, Kyoto University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Horiuchi
authors.forename: H
authors.initials: H
authors.lastname: Saji
authors.forename: Y
authors.initials: Y
authors.lastname: Arano
authors.forename: A
authors.initials: A
authors.lastname: Yokoyama
mesh_terms: D000818:Animals; D004396:Coloring Agents / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D005452:Fluoresceins / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D005982:Glutathione Transferase / Q000378:metabolism*; D007098:Imino Acids / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D008099:Liver / Q000000981:diagnostic imaging* / Q000378:metabolism; D008111:Liver Function Tests; D008297:Male; D051379:Mice; D015609:Organotechnetium Compounds / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D011485:Protein Binding; D011877:Radionuclide Imaging; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
chemical_list: D004396:Coloring Agents; D005452:Fluoresceins; D007098:Imino Acids; D015609:Organotechnetium Compounds; D005982:Glutathione Transferase; C008109:iminodiacetic acid