High-performance liquid chromatographic analysis of imidazolium and pyridinium oximes in plasma and urine.
Imidazolium oximes are useful in the treatment of organophosphorus agent poisoning. However, the extant methods for analyzing oximes in plasma and urine samples are not adequate. A unique high-performance liquid chromatographic assay method was developed for quantitating either imidazolium or pyridinium oximes. Plasma or urine samples can be injected directly onto the column after a centrifugation filtration step. This method demonstrates a different approach in the method development for quaternary ammonium compounds using non-end-capped reversed-phase columns and a mobile phase containing competing cations. In addition, a preliminary pharmacokinetic study of the imidazolium oxime in rabbits was carried out using this method.
Shih
M L
ML
U.S. Army Medical Research Institute of Chemical Defense, Aberdeen Proving Ground, MD 21010-5425.
Smith
J R
JR
Salem
G W
GW
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006207:Half-Life; D007093:Imidazoles / Q000032:analysis* / Q000097:blood / Q000652:urine; D008832:Microchemistry; D010091:Oximes / Q000032:analysis* / Q000097:blood / Q000652:urine; D011726:Pyridinium Compounds / Q000032:analysis* / Q000097:blood / Q000652:urine; D011786:Quality Control; D011817:Rabbits
D007093:Imidazoles; D010091:Oximes; D011726:Pyridinium Compounds; C065159:WR 254416; C065160:WR 255737; C005514:N,N'-monomethylenebis(pyridiniumaldoxime); C022870:asoxime chloride