title: Subcellular distribution of soluble and membrane-bound Leu-, Arg- and Asp-beta-naphthylamide-hydrolysing activities in rat brain.
abstract: Subcellular distribution of soluble and membrane-bound Leu-, Arg- and Asp-beta-naphthylamide hydrolysing activities (arylamidase activity) was studied from left and right rat brains, each including hemisphere, cerebellum and brain stem. Both soluble Leu- and Arg-beta-naphthylamide hydrolysing activities showed the highest levels in the synaptosomal fraction. However, the microsomal fraction presented the highest levels when membrane-bound activity was assayed. When we used Asp-beta-naphthylamide as substrate, there were no differences among fractions in the membrane-bound activity, and the highest soluble activity was present at the mitochondrial level. Two different patterns in the subcellular distribution of enzymatic activity were observed: One of them was the result of the use of Leu- or Arg-beta-naphthylamide as substrate and the other when Asp-beta-naphthylamide was employed. No differences between left and right brains in soluble or membrane-bound activities were found.
authors.affiliation: Department of Physiology, Medical School, University of the Basque Country, Bilbao, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Ramirez
authors.forename: G
authors.initials: G
authors.lastname: Arechaga
authors.forename: P
authors.initials: P
authors.lastname: Lardelli
authors.forename: D
authors.initials: D
authors.lastname: Venzon
authors.forename: J M
authors.initials: JM
authors.lastname: de Gandarias
mesh_terms: D000626:Aminopeptidases / Q000378:metabolism*; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000378:metabolism; D001224:Aspartic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D001921:Brain / Q000201:enzymology*; D002462:Cell Membrane / Q000378:metabolism; D007930:Leucine / Q000031:analogs & derivatives* / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012995:Solubility
chemical_list: D001224:Aspartic Acid; C044335:aspartic acid beta-naphthylamide; C029772:arginine beta-naphthylamide; C040135:leucine-beta-naphthylamide; D001120:Arginine; D000626:Aminopeptidases; D007930:Leucine