Anti-human factor IX monoclonal antibodies specific for calcium ion-induced conformations.
Four monoclonal antibodies have been produced, which are specific for the Ca2+ or Sr2(+)-induced conformation of human factor IX. Certain, but not all, gamma-carboxy-glutamic acid residues in factor IX are involved in the epitope expression together with the conformation stabilized by the adjacent region of Gla-domain and a disulfide bridge. All the antibodies interfered with the binding of factor IX to phospholipids and inhibited the procoagulant activity of factors IX and IXa beta.
Sugo
T
T
Institute of Hematology, Jichi Medical School, Tochigi, Japan.
Mizuguchi
J
J
Kamikubo
Y
Y
Matsuda
M
M
D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D001780:Blood Coagulation Tests; D002118:Calcium / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D005164:Factor IX / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D010743:Phospholipids / Q000378:metabolism; D011487:Protein Conformation
D000911:Antibodies, Monoclonal; D010743:Phospholipids; D005164:Factor IX; D002118:Calcium