title: Restoration of the responsiveness to growth factors in senescent cells by an embryonic cell extract.
abstract: In senescent fibroblast cell cultures which have approached a postmitotic stage in vitro, responsiveness to growth factors is restored upon exposure to an embryonic sheep cell extract. The extract contains molecules below a molecular weight of 1 x 10(5) Da in aqueous solution. Following a transient exposure to the extract, mitotic activity is resumed, and the cells keep dividing over several passages. The target cells which respond to the treatment were identified in a single-cell assay as those that still had the capacity to undergo at least several mitotic divisions before entering the final stage of senescence.
authors.affiliation: Institute for Virus Research, German Cancer Research Center, Heidelberg.
authors.forename: E
authors.initials: E
authors.lastname: Amtmann
authors.forename: E
authors.initials: E
authors.lastname: Eddé
authors.forename: G
authors.initials: G
authors.lastname: Sauer
authors.forename: O
authors.initials: O
authors.lastname: Westphal
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D006224:Cricetinae; D003470:Culture Media; D004622:Embryo, Mammalian / Q000502:physiology*; D006133:Growth Substances / Q000494:pharmacology; D007700:Kinetics; D012756:Sheep; D014020:Tissue Extracts / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D006133:Growth Substances; D014020:Tissue Extracts