Pregnancy suppression by a platelet activating factor antagonist, ONO-6240, in mice.
Mouse embryos produce an embryo-derived platelet activating factor (EDPAF) during their early developmental stages. The present study was aimed at investigating the physiological significance of EDPAF by using a specific PAF antagonist, ONO-6240. EDPAF-induced platelet decrease was antagonized by ONO-6240 in a dose-dependent manner, although this alone did not affect circulating platelet concentrations. ONO-6240, when administered to maternal mice, increased the proportion of mice conceiving small litter sizes, whereas it did not affect in vivo or in vitro development of embryos. EDPAF was suggested to play an important role in establishing pregnancy in mice by promoting implantation.
Ando
M
M
Department of Obstetrics and Gynecology, Ehime University School of Medicine, Japan.
Suginami
H
H
Matsuura
S
S
D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000378:metabolism; D005260:Female; D008098:Litter Size / Q000187:drug effects; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010972:Platelet Activating Factor / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors* / Q000378:metabolism / Q000494:pharmacology; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000187:drug effects*; D013844:Thiazoles
D010972:Platelet Activating Factor; D013844:Thiazoles; C048549:ONO 6240