[An immunohistochemical study on regional immunological response of uterine cervical cancer].
An immunohistochemical analysis was performed to detect MHC class I (HLA-ABC) and class II (HLA-DR) antigens expression on cancer cells from 20 patients with carcinoma of the cervix using monoclonal antibodies to HLA-ABC and DR antigens. Locally infiltrating lymphocytes were also examined with anti-Leu 1,-Leu 2a,-Leu 3a, and -Leu 12. Class I and class II antigens expression on cervical cancer cell lines OMC-1 and OMC-4 were examined in the same way, and the effects of Interferon (IFN)-gamma treatment were evaluated with Cell-EIA tests. The results were as follows. 1) Class I antigens were preserved in many cases, but lost in some. 2) Class II antigens were newly expressed in some cases. 3) Among the many infiltrating T cells, Leu 2a-positive cells were predominant in surrounding the cancer nests which preserved class I antigens, and Leu 3a-positive cells were predominant in surrounding the cancer nests which expressed class II antigens. 4) Both OMC-1 and OMC-4 cervical cancer cell lines expressed class I and class II antigens. 5) Cell-EIA tests showed that IFN-gamma treatment enhanced the expression of class I and class II antigens in OMC-1 and OMC-4. These results suggested that the preservation of class I antigens and the expression of class II antigens on cervical cancer cells markedly increase the local immune response in cervical cancer, and IFN-gamma treatment enhances the immune response in vivo.
Okamoto
Y
Y
Department of Obstetrics and Gynecology, Osaka Medical College.
D000230:Adenocarcinoma / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000276:immunology; D005260:Female; D015395:Histocompatibility Antigens Class I / Q000032:analysis; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D007370:Interferon Type I / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology; D014407:Tumor Cells, Cultured / Q000276:immunology; D002583:Uterine Cervical Neoplasms / Q000276:immunology*
D000945:Antigens, Differentiation, T-Lymphocyte; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D007370:Interferon Type I