Establishment and characterization of a tumorigenic murine vascular endothelial cell line (F-2).
A new murine cell line, designated F-2, was established from an ultraviolet light-induced tumor which developed on the back skin of a BALB/c x C57BL/6 F1-nu/nu nude mouse. More than 1 x 10(5) F-2 cells injected into nude mouse skin produced rapidly developing hemangiomatous lesions. F-2 had a 14-h doubling time under 10% fetal calf serum-containing Dulbecco's modified Eagle's medium culture conditions without any cell growth factor supplementation, and it showed "cobblestone" appearance at confluency. F-2 was able to rapidly differentiate on Matrigel with resultant fine network structure and tubule formation. Ultrastructural observations of the tubule demonstrated that the F-2 cells were connected to each other by intermediate junctions and arranged to form spaces, but that no Weibel-Palade bodies were found in the cytoplasm. F-2 also showed the active uptake of fluorescent-labeled acetylated low-density lipoprotein at 37 degrees C but not at 4 degrees C, and it showed prominent binding to the lectin, Griffonia simplicifolia I agglutinin. The addition of fibroblast growth factor did not facilitate the growth of F-2 cells. These findings strongly suggest that F-2 is a transformed cell line with tumorigenicity and vascular endothelial cell properties, and it may be useful in the study of vascular tumor biology.
Toda
K
K
Department of Dermatology, Kyoto University, Japan.
Tsujioka
K
K
Maruguchi
Y
Y
Ishii
K
K
Miyachi
Y
Y
Kuribayashi
K
K
Imamura
S
S
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D046508:Culture Techniques / Q000379:methods; D004730:Endothelium, Vascular / Q000187:drug effects / Q000473:pathology* / Q000528:radiation effects; D005346:Fibroblast Growth Factors / Q000494:pharmacology; D006391:Hemangioma / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D009381:Neoplasms, Radiation-Induced / Q000473:pathology*; D012867:Skin / Q000528:radiation effects*; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects; D014466:Ultraviolet Rays
D005346:Fibroblast Growth Factors