Alteration of lipid methylation by oleic acid in rat heart sarcolemma.
Incubation of rat heart sarcolemma with the methyl donor S-adenosyl-L-[methyl-3H] methionine resulted in N-methylation of phosphatidylethanolamine and methylation of a heterogenous fraction of nonpolar lipids in the membrane. Oleic acid reduced the synthesis of N-methylated phospholipids and stimulated the methyl group incorporation into nonpolar lipids in a concentration-dependent manner. Both methylation reactions were not affected when oleic acid was substituted by methyl ester of oleic acid or by the detergents sodium deoxycholate or Triton X-100. This study suggests that the enzymatic biosynthesis of the N-methylated phospholipids may be altered by free fatty acids.
Vetter
R
R
Division of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, Winnipeg, Canada.
Dai
J
J
Panagia
V
V
Dhalla
N S
NS
D000818:Animals; D003840:Deoxycholic Acid / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006321:Heart / Q000187:drug effects; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism*; D008745:Methylation; D008780:Methyltransferases / Q000378:metabolism*; D009206:Myocardium / Q000201:enzymology*; D017830:Octoxynol; D019301:Oleic Acid; D009829:Oleic Acids / Q000008:administration & dosage / Q000494:pharmacology*; D010743:Phospholipids / Q000378:metabolism; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012436:S-Adenosylmethionine / Q000378:metabolism; D012508:Sarcolemma / Q000187:drug effects / Q000201:enzymology*
D003902:Detergents; D008563:Membrane Lipids; D009829:Oleic Acids; D010743:Phospholipids; D003840:Deoxycholic Acid; D019301:Oleic Acid; D011092:Polyethylene Glycols; D012436:S-Adenosylmethionine; D017830:Octoxynol; D008780:Methyltransferases