title: Deletion mapping of 39 random isolated Y-chromosome DNA fragments.
abstract: Thirty-nine recombinants isolated from a Y chromosome-specific library were deletion mapped. Seven deletion intervals were defined by hybridization of probes to DNA of eight individuals with aberrant Y chromosomes. Extreme cytogenetic limits of the deletion intervals were determined by in situ hybridization of one probe per deletion interval. Five intervals, with a total of twenty-five probes, were allocated to the long-arm euchromatic region. The probes described will be useful for characterization of aberrant Y chromosomes, in searching for expressed sequences on the Y chromosome, and for further study of the evolutionary relationship between the Y chromosome and other chromosomes.
authors.affiliation: Department of Human Genetics, Tygerberg Hospital, Republic of South Africa.
authors.forename: C J
authors.initials: CJ
authors.lastname: Oosthuizen
authors.forename: J S
authors.initials: JS
authors.lastname: Herbert
authors.forename: L K
authors.initials: LK
authors.lastname: Vermaak
authors.forename: J
authors.initials: J
authors.lastname: Brusnicky
authors.forename: J
authors.initials: J
authors.lastname: Fricke
authors.forename: L
authors.initials: L
authors.lastname: du Plessis
authors.forename: A E
authors.initials: AE
authors.lastname: Retief
mesh_terms: D002872:Chromosome Deletion; D002874:Chromosome Mapping; D015342:DNA Probes; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D012303:Ring Chromosomes; D014998:Y Chromosome / Q000648:ultrastructure*
chemical_list: D015342:DNA Probes