title: A spectroelectrochemical study of microperoxidase at bare and gold-plated RVC thin-layer electrodes.
abstract: Direct electrochemistry of microperoxidase (the heme-undecapeptide from cytochrome c) has been followed at a bare and a gold plated RVC thin-layer electrode, using the spectropotentiostatic method or voltabsorptometry. Both techniques yield 'clean' and undistorted signals; their analysis easily provides quantitative information for the electrochemical parameters of microperoxidase and shows that spectroelectrochemistry is a powerful method to study the redox behavior of metalloproteins or their active site fragments.
authors.affiliation: Department of Chemistry, University of Camerino, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Zamponi
authors.forename: R
authors.initials: R
authors.lastname: Santucci
authors.forename: M
authors.initials: M
authors.lastname: Brunori
authors.forename: R
authors.initials: R
authors.lastname: Marassi
mesh_terms: D002244:Carbon; D004563:Electrochemistry; D004566:Electrodes; D006046:Gold; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D010544:Peroxidases; D013053:Spectrophotometry
chemical_list: D002244:Carbon; D006046:Gold; D010544:Peroxidases; C021799:microperoxidase