title: Chlamydial infection of subcutaneous conjunctival transplants in guinea pigs.
abstract: The development and testing of candidate vaccines for trachoma are constrained because only humans and nonhuman primates are susceptible to conjunctival infection with Chlamydia trachomatis. Guinea pig inclusion conjunctivitis (GPIC), an analogous disease of guinea pigs, provides a useful, less expensive model to study ocular chlamydial infections. GPIC is caused by a Chlamydia psittaci strain whose external constituents are very similar to those of C. trachomatis. To develop a better model for studying GPIC immunity, conjunctival pockets were established under the abdominal skin of guinea pigs by subcutaneous implantation. Up to six implants could be produced in each animal. The success rate of implantation was 79.0% (n = 148). These pockets were then infected with GPIC. The organism was recovered from the autografts indicating local replication, and tests for serum antibody by microimmunofluorescence showed production of GPIC-specific antibody of IgG and IgM classes after infection. There was minimal antibody response after moderate inoculating doses to the implants, and the titers increased more slowly than after eye infection with GPIC; with higher concentration of the inoculum, however, the antibody response increased to the same levels as with the ocular challenge but more slowly. Inoculation of pockets with GPIC also produced acute inflammatory changes in infected autografts (n = 101). Histologic examination of infected grafts showed chlamydial inclusions in epithelial cells and significant infiltration with lymphocytes and polymorphonuclear cells. Subcutaneous autografts may provide a useful model for chronologic studies of chlamydial infection. The delayed immunologic response, however, suggests that these pockets of implanted epithelium do not have full access to the immune system.
authors.affiliation: Francis I. Proctor Foundation, University of California, San Francisco 94143-0412.
authors.forename: R T
authors.initials: RT
authors.lastname: Pham
authors.forename: M
authors.initials: M
authors.lastname: Sung
authors.forename: C R
authors.initials: CR
authors.lastname: Dawson
authors.forename: J
authors.initials: J
authors.lastname: Schachter
mesh_terms: D000005:Abdomen; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D002691:Chlamydophila psittaci / Q000302:isolation & purification; D003228:Conjunctiva / Q000637:transplantation*; D003231:Conjunctivitis / Q000276:immunology / Q000473:pathology; D062109:Dermatologic Surgical Procedures; D004195:Disease Models, Animal; D006168:Guinea Pigs; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D009956:Psittacosis / Q000276:immunology / Q000473:pathology; D014182:Transplantation, Autologous; D016042:Transplantation, Heterotopic
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M