title: Phosphorylation of stathmin and other proteins related to nerve growth factor-induced regulation of PC12 cells.
abstract: We previously identified a set of soluble proteins whose phosphorylation could be originally related to the multihormonal regulations of anterior pituitary cells. Among these proteins, stathmin (proteins 7 and 8) was found to be ubiquitous and mostly abundant in neurons. Interestingly, stathmin and some other phosphoproteins of the same set could be identified also in PC12 cells in culture. Their phosphorylation was stimulated in these cells by nerve growth factor (NGF) in a way associated with its short term actions, probably corresponding to the early steps of its neuronal differentiating activity. In addition, the same proteins had their phosphorylation stimulated in the presence of fibroblast growth factor, known to stimulate PC12 cell differentiation in a way similar to NGF. A pharmacological analysis allowed us to distinguish three characteristic subsets of phosphoproteins, respectively, affected by cAMP-dependent agents, by cAMP-independent ones, or by both types of agents. Moreover, phosphorylation of stathmin and some other proteins was additive in the presence of NGF and of the cAMP-promoting agent forskolin. Altogether, the present results unravel some intracellular mechanisms related to the regulation of PC12 cells by extracellular effectors. They extend to the regulation of cell differentiation in our recent model for stathmin (Sobel, A., Boutterin, M-C., Beretta, L., Chneiweiss, H., Doye, V., and Peyro-Saint-Paul, H. (1989) J. Biol. Chem. 264, 3765-3772) as an ubiquitous intracellular relay possibly integrating the actions of diverse second messenger pathways involved in cell regulations.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale U 153, Paris, France.
authors.forename: V
authors.initials: V
authors.lastname: Doye
authors.forename: M C
authors.initials: MC
authors.lastname: Boutterin
authors.forename: A
authors.initials: A
authors.lastname: Sobel
mesh_terms: D000241:Adenosine / Q000494:pharmacology; D000310:Adrenal Gland Neoplasms; D000818:Animals; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional; D008868:Microtubule Proteins; D009363:Neoplasm Proteins / Q000378:metabolism*; D009414:Nerve Growth Factors / Q000494:pharmacology*; D010673:Pheochromocytoma; D010750:Phosphoproteins / Q000302:isolation & purification / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D050777:Stathmin; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism*
chemical_list: D008868:Microtubule Proteins; D009363:Neoplasm Proteins; D009414:Nerve Growth Factors; D010750:Phosphoproteins; D050777:Stathmin; D005576:Colforsin; D000241:Adenosine; D013755:Tetradecanoylphorbol Acetate