The influence of oligonucleotide-effector on the selectivity of sequence specific modification of 16 S rRNA.
The influence of duplex stabilizing oligonucleotide-effector (oligonucleotide, carrying N-(2-hydroxyethyl)phenazinium residues on both ends), on selectivity of site-directed modification of E. coli 16 S rRNA (1542 nucleotides in length) under the conditions of its secondary structure stability was studied. The constant of cooperative binding of the reagent and the oligonucleotide-effector with 16 S rRNA was determined. The accuracy of modification was shown to double in the presence of 50 microM effector at 5 microM concentration of the reagent.
Zenkova
M A
MA
Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of USSR Academy of Sciences.
Fedorova
O S
OS
Levina
A S
AS
Mamaev
S V
SV
Karpova
G G
GG
D000477:Alkylating Agents; D001483:Base Sequence; D006860:Hydrogen Bonding; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D012335:RNA, Ribosomal / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
D000477:Alkylating Agents; D015345:Oligonucleotide Probes; D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S