T cell depletion from human bone marrow using magnetic beads.
We have studied a recently described method to purge human marrow of T cells using magnetic beads conjugated to various anti-T cell monoclonal antibodies. Using anti-CD2 and anti-T cell receptor/CD3 monoclonal antibodies, we show that up to 2.76 log T cell purge can be achieved while recovery of hemopoietic activity is 82%. As a control, our clinically established T cell depletion protocol, namely soybean agglutination combined with E-rosetting, provided 3.39 log T cell purge and 59% recovery of hemopoietic activity. The impact of these results on T cell depletion strategies in HLA-nonidentical bone marrow transplantation is discussed.
Knobloch
C
C
Department of Pediatrics II, University of Ulm, FRG.
Spadinger
U
U
Rueber
E
E
Friedrich
W
W
D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation / Q000276:immunology / Q000379:methods*; D005069:Evaluation Studies as Topic; D006086:Graft vs Host Disease / Q000517:prevention & control; D006680:HLA Antigens; D006239:Haplotypes; D006801:Humans; D037102:Lectins; D008212:Lymphocyte Depletion; D008280:Magnetics; D037121:Plant Lectins; D012397:Rosette Formation; D030262:Soybean Proteins; D013601:T-Lymphocytes / Q000276:immunology; D010835:Phytohemagglutinins
D006680:HLA Antigens; D037102:Lectins; D037121:Plant Lectins; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins