[The trypsin cleavage of hemagglutinin enhances the infectivity of the influenza virus in mice].
Mouse-pathogenic influenza A/Aichi 2/68 (H3N2) grown in cell culture and having uncleaved hemagglutinin HA after treatment with trypsin underwent proteolytic shearing of HA (m.w. 75 kD) into two fragments: NAL (60 kD) and HA2 (15 kD); its lethal effect on mice inoculated intranasally increased more than 200-fold. The virus treated with chemotrypsin underwent similar shearing of HA into HAL and HA2; however, its lethal effect on mice was weak, analogous to that of intact virus with uncleaved HA.
Zhirnov
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D000818:Animals; D002918:Chymotrypsin / Q000494:pharmacology; D006389:Hemagglutinins, Viral / Q000502:physiology*; D009980:Influenza A virus / Q000187:drug effects* / Q000472:pathogenicity; D007928:Lethal Dose 50; D051379:Mice; D009976:Orthomyxoviridae Infections / Q000382:microbiology; D014357:Trypsin / Q000494:pharmacology*; D014774:Virulence / Q000187:drug effects; D014775:Virus Activation / Q000187:drug effects; D014776:Virus Cultivation
D006389:Hemagglutinins, Viral; D002918:Chymotrypsin; D014357:Trypsin
Rasshcheplenie gemagglutinina tripsinom povyshaet infektsionnost' virusa grippa dlia mysheĭ.