[I-125 labeling of human, rat, and bovine fibrinogen].
Human, rat and bovine fibrinogen were radioiodinated using a modified chloramine-T method under mild labelling conditions and with a satisfactory labelling efficiency. The 125I-human, rat and bovine fibrinogen preparations obtained met the requirements with respect to clottability, low content of 125Iodide as well as stability in vitro and in vivo. SDS-PAGE mobility demonstrated that the integrity of the fibrinogen molecule was not affected by the labelling procedure.
Richter
M
M
Institut für Pharmakologie und Toxikologie, Medizinischen Akademie Erfurt.
Cyranka
U
U
Markwardt
F
F
D000818:Animals; D002417:Cattle; D002855:Chromatography, Thin Layer; D004591:Electrophoresis, Polyacrylamide Gel; D005340:Fibrinogen / Q000032:analysis*; D006801:Humans; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D051381:Rats; D011919:Rats, Inbred Strains
D007457:Iodine Radioisotopes; D005340:Fibrinogen
125I-Markierung von Human-, Ratten- und Rinderfibrinogen.