Endopolyploidy in follicle epithelial cells around the nurse chamber during oogenesis in Chrysomya putoria (Diptera, Calliphoridae).
Feulgen-DNA values and nuclear areas were evaluated microspectrophotometrically for epithelial cells of the ovarian follicle during oogenesis in Chrysomya putoria. The aim was to investigate whether polyploidization occurred in the cells surrounding the nurse chamber and/or in those around the oocyte as well as whether different DNA amounts were found regarding the cell types considered. Four DNA endoreduplicative cycles could be demonstrated for the epithelial cells regardless of their localization on the follicle, during oogenesis. A small percentage of epithelial cells reaches a 32C degree. The nuclear area, however, did not increase at the same rate in cells covering the oocyte as in those covering the nurse chamber, in some of the oogenesis stages. The meaning of endopolyploidy for these cells is discussed, considering reports on relevance of secretory activities and their maintenance in polyploid cell systems.
Avancini
R M
RM
Department of Parasitology, Universidade Estadual de Campinas, Brazil.
Mello
M L
ML
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D004396:Coloring Agents; D004247:DNA / Q000378:metabolism*; D004175:Diptera / Q000235:genetics*; D004848:Epithelium / Q000648:ultrastructure; D005260:Female; D009866:Oogenesis / Q000235:genetics*; D006080:Ovarian Follicle / Q000166:cytology; D011123:Polyploidy; D012394:Rosaniline Dyes
D004396:Coloring Agents; C007722:Feulgen stain; D012394:Rosaniline Dyes; D004247:DNA