title: Increase of muscarinic receptor following kainic acid lesions of the nucleus basalis magnocellularis in rat brain: an autoradiographic study.
abstract: We observed changes in cholinergic markers in rat brain seven days after lesioning the nucleus basalis magnocellularis (nbm) with kainic acid. In histochemical preparations stained for acetylcholinesterase (AChE), there was a marked loss of large AChE reactive neurons within and beneath the nbm on the injected side, and the AChE positive fibers were greatly decreased particularly in the IV-VI layers of the frontal and parietal cortices ipsilateral to the kainate lesion. Using in vitro receptor autoradiography, we found a significant increase (about 25%) in 3H-QNB binding sites in the I-IV layers of the ipsilateral frontal and parietal cortices (p 0.05, Student's t-test). The area with decreased AChE activity and increased density in 3H-QNB binding sites corresponded to the innervation of the cholinergic system arising from the nbm. The increase of density in 3H-QNB binding sites was considered to reflect the postsynaptic denervation supersensitivity.
authors.affiliation: Third Department of Internal Medicine, Hiroshima University School of Medicine, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Katayama
authors.forename: S
authors.initials: S
authors.lastname: Kito
authors.forename: Y
authors.initials: Y
authors.lastname: Yamamura
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D001479:Basal Ganglia / Q000187:drug effects*; D001665:Binding Sites; D006651:Histocytochemistry; D007608:Kainic Acid / Q000633:toxicity*; D008297:Male; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011976:Receptors, Muscarinic / Q000187:drug effects* / Q000378:metabolism; D013377:Substantia Innominata / Q000187:drug effects* / Q000293:injuries / Q000378:metabolism
chemical_list: D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate; D000110:Acetylcholinesterase; D007608:Kainic Acid