title: Effects induced in neutrophils by a precursor of triplet acetone.
abstract: The addition of a precursor of the enol form of isobutanal to neutrophils results in formation of triplet acetone, as attested to by emission from appropriate acceptors and cell damage (Nascimento et al., 1986 Biochim. Biophys. Acta 888, 337-342). The present study confirms the formation of triplet acetone by detection of the direct emission (lambda max 430 nm) and differentiates between effects produced by triplet acetone and by the enol substrate itself. Thus, triplet acetone: (1) enhances the release of ribonucleic acid; (2) promotes lipid peroxidation (N3(-)-inhibitable formation of thiobarbituric acid reactive products and concomitant light emission peaking at 480-500 nm); (3) increases myeloperoxidase activity, presumable as a result of damage and consequent increased exposure of the enzyme. On the other hand, the enol greatly enhances the release of protein(s) into the medium. These results confirm the utility of the neutrophil as a model system for the study of chemiexcitation processes induced at the cellular level. They also provide the first demonstration that an excited species formed at the cellular level may induce release of nucleic acids, thus reflecting the occurrence of deleterious processes in situ.
authors.affiliation: Departamento de Bioquímica, Universidade de São Paulo, Brazil.
authors.forename: J A
authors.initials: JA
authors.lastname: Escobar
authors.forename: G
authors.initials: G
authors.lastname: Cilento
authors.forename: A L
authors.initials: AL
authors.lastname: Nascimento
mesh_terms: D000433:1-Propanol / Q000378:metabolism / Q000494:pharmacology; D000096:Acetone / Q000031:analogs & derivatives*; D000447:Aldehydes / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D003500:Cyclization; D006861:Hydrogen Peroxide / Q000494:pharmacology; D049449:Luminescence; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D020005:Propanols; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000447:Aldehydes; D020005:Propanols; C037881:triplet acetone; D000096:Acetone; D000433:1-Propanol; D006861:Hydrogen Peroxide; C017439:isobutyraldehyde