The distribution of oleic acid between chylomicron-like emulsions, phospholipid bilayers, and serum albumin. A model for fatty acid distribution between lipoproteins, membranes, and albumin.
In the process of lipoprotein lipolysis, masses of fatty acid are generated at the surface of the lipoprotein. The newly generated fatty acid may at least partly redistribute from the site of lipolysis to phospholipid-rich membranes and to albumin. We have studied the distribution of [1-13C]oleic acid in model systems consisting of chylomicron-like triacylglycerol-rich emulsions, unilamellar phosphatidylcholine vesicles, and bovine serum albumin. By using high resolution 13C NMR spectroscopy it was possible to distinguish fatty acid in each compartment (emulsion, vesicle, albumin) and quantitate the fatty acid distribution under various conditions of lipid compartment concentration and aqueous pH. When emulsions and vesicles were present in equivalent mass amounts, fatty acid exhibited a profound preference for the lipid bilayers. The release of oleic acid to phospholipid bilayers was presumably also a function of its high molar stoichiometry (5:1) with the albumin present. More equitable distributions of fatty acid between vesicles and emulsions were seen when higher concentrations of emulsion were used. The distribution of fatty acid between compartments was in good agreement with predictions made using the apparent ionization constant, expressed as pKapp, of 7.5 and the surface to core (phospholipid to triacylglycerol) distribution coefficient of 7.0, measured for unionized oleic acid in chylomicron particles (Spooner, P. J. R., Bennett Clark, S., Gantz, D. L., Hamilton, J. A., and Small, D. M. (1988) J. Biol. Chem. 263, 1444-1455). These results indicate that the affinities of fatty acid for phospholipid bilayer and chylomicron-like emulsion surfaces are equivalent. Redistribution of lipolytically generated fatty acid from chylomicron surface to cell membrane may simply be driven by the predominant quantity of the cell membrane surfaces.
Spooner
P J
PJ
Department of Biophysics, Boston University School of Medicine, Housman Medical Research Center, Massachusetts 02118-2394.
Gantz
D L
DL
Hamilton
J A
JA
Small
D M
DM
D002462:Cell Membrane / Q000378:metabolism*; D002914:Chylomicrons; D004655:Emulsions; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008051:Lipid Bilayers; D008074:Lipoproteins / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008854:Microscopy, Electron; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism*; D010743:Phospholipids; D012709:Serum Albumin / Q000378:metabolism*; D012995:Solubility
D002914:Chylomicrons; D004655:Emulsions; D008051:Lipid Bilayers; D008074:Lipoproteins; D009829:Oleic Acids; D010743:Phospholipids; D012709:Serum Albumin; D019301:Oleic Acid