Effect of beta-naphthoflavone and MCPA on liver and kidney drug-metabolizing enzymes from the carp, Cyprinus carpio.
The effects of beta-naphthoflavone (beta-NF) and a chlorophenoxyacetic acid herbicide (MCPA) on hepatic and renal monooxygenase activities and conjugating enzymes from immature carp (Cyprinus carpio) were studied. beta-NF increased hepatic monooxygenase activities but the patterns of differential induction generally obtained in rat liver microsomes with two series of homologous substrates, alkoxycoumarins and alkylresorufins, were not found to be similar in carp liver microsomes. On the other hand, MCPA caused no changes in oxidative metabolism, with the exception of decreased aryl hydrocarbon hydroxylase activity. Renal activities were not modified by MCPA, while beta-NF treatment resulted in marked increases in monooxygenase activities with alkylresorufins as substrates. No changes were found in conjugation activities after treatment with MCPA or beta-NF. These results indicate that (a) the herbicide MCPA should have no effect on drug-metabolizing enzymes from carp, and (b) the hepatic and renal monoxygenase activities of carp are responsive to beta-NF, allowing their use in monitoring water pollution.
Riviere
J L
JL
Institut National de la Recherche Agronomique, Laboratoire d'Ecotoxicologie INRA-ENVL, Marcy l'Etoile, France.
Devaux
A
A
Gonin
O
O
Monod
G
G
D008456:2-Methyl-4-chlorophenoxyacetic Acid / Q000494:pharmacology*; D000818:Animals; D001571:Benzoflavones / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D002347:Carps / Q000378:metabolism*; D003530:Cyprinidae / Q000378:metabolism*; D005419:Flavonoids / Q000494:pharmacology*; D006016:Glycolates / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects / Q000201:enzymology*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D009929:Organ Size / Q000187:drug effects; D013347:Subcellular Fractions / Q000201:enzymology; D019324:beta-Naphthoflavone
D001571:Benzoflavones; D005419:Flavonoids; D006016:Glycolates; D019324:beta-Naphthoflavone; D008456:2-Methyl-4-chlorophenoxyacetic Acid