Crystallization and preliminary X-ray analysis of gamma-aminobutyric acid transaminase.
gamma-Aminobutyric acid transaminase from pig liver, an alpha 2 dimeric enzyme of Mr 110,100, has been crystallized by the vapour diffusion method with polyethylene glycol as precipitant. The crystals are monoclinic, space group P2(1), unit cell dimensions a = 82.1 A, b = 230.0 A, c = 70.3 A, beta = 123.9 degrees and diffract to 2.5 A resolution. There are two dimers per asymmetric unit.
Marković-Housley
Z
Z
Department of Structural Biology, University of Basel, Switzerland.
Schirmer
T
T
Fol
B
B
Jansonius
J N
JN
De Biase
D
D
John
R A
RA
D000612:4-Aminobutyrate Transaminase / Q000302:isolation & purification*; D000818:Animals; D003460:Crystallization; D008099:Liver / Q000201:enzymology*; D011092:Polyethylene Glycols; D011487:Protein Conformation; D013552:Swine; D014961:X-Ray Diffraction
D011092:Polyethylene Glycols; D000612:4-Aminobutyrate Transaminase