[Sensitivity of various study technics in blood stains].
Quadratic pieces of fleece measuring 16 mm2 were soaked with 10 different blood-samples in the dilution steps of 1:1, 1:10, 1:100, 1:1000, respectively, and were tested in blood group typing and identification tests of forensic serology. The above spezified dilutions correspond with 5 microliters, 0.5 microliter, 0.05 microliter and 0.005 microliter of blood, respectively. The detection limit of the microspectrometric test for blood was the dilution 1:10, of the porphyrine test a dilution above 1:100, whereas the preliminary test for blood (peroxidase) succeeded always up to a dilution of 1:1000 and the species determination by the radial immunodiffusion test in agar gels succeeded in most cases op to a dilution of 1:1000. The detection limit of the anti-human globulin inhibition test was between the dilution steps 1:10 and 1:100 when non-titrated and undiluted anti-human globulin serum was used. Gc- and ABO-grouping were possible up to a dilution of 1:100 and were thus the most sensitive grouping systems. Phenotyping of the enzyme-systems and the Gm/Km-system usually required stains with considerably higher blood concentrations i.e. stains of undiluted blood.
Förster
R
R
Kriminaltechnischen Institut, Landeskriminalamtes, Baden-Württemberg, Stuttgart.
Bruder
W
W
D000017:ABO Blood-Group System / Q000235:genetics; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D001798:Blood Proteins / Q000235:genetics*; D001801:Blood Stains; D006801:Humans; D007126:Immunoglobulin Allotypes / Q000235:genetics; D007129:Immunoglobulin Gm Allotypes / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics; D011110:Polymorphism, Genetic / Q000235:genetics; D014809:Vitamin D-Binding Protein / Q000235:genetics
D000017:ABO Blood-Group System; D001798:Blood Proteins; D007126:Immunoglobulin Allotypes; D007129:Immunoglobulin Gm Allotypes; D007527:Isoenzymes; D014809:Vitamin D-Binding Protein
Sensitivität verschiedener Untersuchungstechniken bei Blutspuren.