title: Is the subunit the minimal function unit of creatine kinase?
abstract: The dimeric rabbit muscle isozyme of creatine kinase (MM) is modified by iodoacetamide to produce the inactive dimer (M'M') and then hybridized with native dimeric brain isozyme (BB). The hybrid enzyme (M'B), as isolated by PAGE, has the same Km for both ATP and creatine but half the specific activity of the brain isozyme (BB). Likewise, the hybrid of the modified brain with the native muscle isozyme (MB') has half the activity of the native muscle enzyme. The M'B, MB' and MB hybrid dimers all have essentially the same electrophoretic properties, and their intrinsic fluorescence and CD spectra in the far-ultraviolet region are very similar to those of the homodimers MM and BB. Similar results were obtained for the hybrid (M"B) containing the muscle enzyme subunit modified at both the thiol group with iodoacetamide and the Trp residue with dimethyl(2-hydroxy-5-nitrobenzyl)sulfonium bromide and the native brain enzyme submit. The above results suggest strongly the independent catalytic function of the subunit of creatine kinase.
authors.affiliation: Department of Biological Science and Biotechnology, Tsinghua University, Beijing, China.
authors.forename: X C
authors.initials: XC
authors.lastname: Wang
authors.forename: H M
authors.initials: HM
authors.lastname: Zhou
authors.forename: Z X
authors.initials: ZX
authors.lastname: Wang
authors.forename: C L
authors.initials: CL
authors.lastname: Tsou
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology*; D002942:Circular Dichroism; D003402:Creatine Kinase / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D007527:Isoenzymes; D007700:Kinetics; D046911:Macromolecular Substances; D009132:Muscles / Q000201:enzymology*; D011487:Protein Conformation; D011817:Rabbits
chemical_list: D007527:Isoenzymes; D046911:Macromolecular Substances; D003402:Creatine Kinase