[A comparative study of the karyotypes of fetuses of spontaneous early abortions obtained by direct method and by long-term culture].
The direct method of chromosomal preparations on chorionic villi biopsies has been applied to the cytogenetic study of 34 early spontaneous abortion products. These results were compared with those obtained through long term cultures performed simultaneously from the same samples. The success rate obtained through the direct method (86.2%) is superior to that of the long term culture (76.4%). However, the combined use of both techniques further improves the success rate (94.1%) and the reliability of the results. Among the 32 informative cases, 15 chromosomal aberrations (46.8%) have been detected. These data demonstrate the value of the direct method of karyotyping chorionic villi from spontaneous abortion products. However, considering the specific advantages of either method respectively, the authors recommend that both be used simultaneously for optimal diagnostic efficiency.
Ogur
G
G
Laboratoire de Cytogénétique, Hôpital Universitaire Brugmann, Université Libre de Bruxelles, Belgique.
Debusscher
C
C
Vamos
E
E
D000022:Abortion, Spontaneous / Q000235:genetics*; D000328:Adult; D002824:Chorionic Villi / Q000648:ultrastructure; D015193:Chorionic Villi Sampling; D002869:Chromosome Aberrations / Q000235:genetics; D025063:Chromosome Disorders; D002875:Chromosomes / Q000032:analysis / Q000648:ultrastructure*; D046508:Culture Techniques; D005260:Female; D006801:Humans; D007621:Karyotyping; D011247:Pregnancy; D013997:Time Factors
Etude comparée des caryotypes de produits d'avortements spontanés précoces obtenus par une méthode directe et par culture à long terme.