Thermochemotherapy for B16 melanoma: combination therapy of hyperthermia, melphalan, and CCNU in mice.
We studied, by means of quantitative histopathological methods, the changes that took place in B16 melanoma implanted into C57BL/6J mice after combination treatment of hyperthermia, melphalan, and 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea (CCNU). The studies were made on animals sacrificed on days 11, 18, and 25 after implantation. Tumors in treated animals showed a progressive delay in growth, a noticeably reduced number of metastases, and diminution of proliferative capacity. However, this treatment did not affect necrosis, stroma, capsule, or cell infiltration. Ultrastructurally, signs of cell damage were constantly present.
Vicente
V
V
Department of Pathology, Faculty of Medicine, University of Murcia, Spain.
Gomez
M
M
Ochotorena
M M
MM
Cremades
A
A
Canteras
M
M
D000818:Animals; D003131:Combined Modality Therapy; D006979:Hyperthermia, Induced; D008130:Lomustine / Q000627:therapeutic use*; D008546:Melanoma, Experimental / Q000188:drug therapy / Q000628:therapy* / Q000648:ultrastructure; D008558:Melphalan / Q000627:therapeutic use*; D051379:Mice; D008810:Mice, Inbred C57BL; D008940:Mitotic Index; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D012044:Regression Analysis
D008130:Lomustine; D008558:Melphalan