Simultaneous determination of ginsenosides and saikosaponins by high-performance liquid chromatography.
Octadecyl porous glass was used as the packing for reversed-phase high-performance liquid chromatography. A mixture of ginsenosides and saikosaponins (saponins of ginseng and bupleurum root, respectively) were analysed with detection at 203 nm. A well resolved chromatogram of ginsenoside Rb1, Rc, Rb2 and Rd and saikosaponin a, b2 and c was obtained with acetonitrile-water (25.5:74.5) as the mobile phase. The whole separation was achieved in 20 min with a flow-rate of 1.5 ml/min. Calibration graphs for ginsenoside Rb1, Rc, Rb2 and Rd and saikosaponin a and c were linear up to 5 micrograms. Rapid and accurate simultaneous determinations of the saponins are possible by the described method.
Kanazawa
H
H
Kyoritsu College of Pharmacy, Tokyo, Japan.
Nagata
Y
Y
Matsushima
Y
Y
Tomoda
M
M
Takai
N
N
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D036145:Ginsenosides; D006035:Glycyrrhiza / Q000032:analysis; D007166:Immunosuppressive Agents / Q000032:analysis*; D009828:Oleanolic Acid / Q000031:analogs & derivatives*; D005894:Panax / Q000032:analysis; D010944:Plants / Q000032:analysis*; D010946:Plants, Medicinal; D012502:Sapogenins / Q000032:analysis*; D012503:Saponins / Q000032:analysis*
D036145:Ginsenosides; D007166:Immunosuppressive Agents; D012502:Sapogenins; D012503:Saponins; D009828:Oleanolic Acid; C025759:saikosaponin D