Sequencing reactions in microtiter plates.
We describe a simple and inexpensive method of performing sequencing reactions for 24 single-strand M13 DNA clones in microtiter plates. To simplify elevated temperature incubations during sequencing reactions, two heating blocks were designed to accommodate microtiter plates and fit within common laboratory heating modules. With only slight modification of standard fluorescent and radioisotopic sequencing methods, the sequencing reactions for 24 clones can be done in as little as 40 minutes.
Koop
B F
BF
Division of Biology, California Institute of Technology, Pasadena 91125.
Wilson
R K
RK
Chen
C
C
Halloran
N
N
Sciammis
R
R
Hood
L
L
Lindelien
J W
JW
D001435:Bacteriophages / Q000032:analysis; D004274:DNA, Recombinant / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D008832:Microchemistry / Q000295:instrumentation; D009710:Nucleotide Mapping / Q000295:instrumentation / Q000379:methods*; D013696:Temperature
D004274:DNA, Recombinant; D004279:DNA, Viral