Chlamydial heat shock proteins and trachoma.
Two chlamydial proteins (HSP-60 and HSP-70) have marked homology with bacterial and mammalian heat shock proteins. Previous studies have indicated that when inoculated into the eyes of immune animals, a Triton X-100 extract of chlamydia containing HSP-60 induces an ocular delayed-type hypersensitivity reaction. The potential for HSP-70 to induce a similar reaction was tested in six cynomolgus monkeys that had been sensitized to both antigens by previous ocular chlamydial infection. Whereas the chlamydial extract containing HSP-60 induced a marked clinical response within 24 h of inoculation, no response followed inoculation of HSP-70 in the contralateral eye. The lack of a response to HSP-70 suggests that further assessment of its potential as a trachoma vaccine is warranted.
Taylor
H R
HR
Dana Center for Preventive Ophthalmology, Johns Hopkins University, Baltimore, Maryland 21205.
Maclean
I W
IW
Brunham
R C
RC
Pal
S
S
Whittum-Hudson
J
J
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology; D002692:Chlamydia trachomatis; D015818:Eye Infections, Bacterial / Q000139:chemically induced* / Q000150:complications; D006360:Heat-Shock Proteins / Q000633:toxicity*; D006968:Hypersensitivity, Delayed / Q000139:chemically induced* / Q000150:complications; D008252:Macaca fascicularis; D014141:Trachoma / Q000139:chemically induced* / Q000150:complications
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D006360:Heat-Shock Proteins