Synthesis of new steroid haptens for radioimmunoassay--VIII. Development and validation of a specific radioimmunoassay for serum 5 alpha-androstane-3 alpha, 17 beta-diol 17-glucuronide.
5 alpha-Androstane-3 alpha, 17 beta-diol glucuronide (androstanediol-G) is a dihydrotestosterone metabolite whose serum levels are elevated in hirsute women. Current assay methods do not distinguish between the two androstanediol-G isomers, androstanediol 3-G and androstanediol 17-G. Since the production of these isomers may be influenced by different factors, we have developed a specific radioimmunoassay for androstanediol 17G. The antibody was raised against 5 alpha-androstane-3 alpha, 17 beta-diol 17-G conjugated to bovine serum albumin (BSA). [9,11(-3H]5 alpha-Androstane-3 alpha, 17 beta-diol 17-G was used for determination of procedural losses and as the labeled ligand in the assay. Unlabeled androstanediol 17-G was used as assay standard. Serum levels of total androstanediol-G, androstanediol 3-G and androstanediol 17-G were measured in 8 normal men. Total androstanediol-G levels were 16.5 +/- 5.2 nmol/l, androstanediol 17-G levels were 12.9 +/- 5 nmol/l, and androstanediol 3-G levels were 3.3 +/- 1.8 nmol/l. 77 +/- 13% of total androstanediol-G was androstanediol 17-G. These results confirm our previous findings that androstanediol 17-G is the predominant androstanediol-G isomer in human serum and suggests that 5 alpha-dihydrotestosterone (DHT) is preferentially metabolized to androstanediol 17-G.
Thompson
D L
DL
Department of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada.
Rittmaster
R S
RS
Rodriguez
A M
AM
Moore
P H
PH
Rao
P N
PN
D015113:Androstane-3,17-diol / Q000031:analogs & derivatives / Q000097:blood* / Q000276:immunology; D000732:Androstanols / Q000097:blood*; D000918:Antibody Specificity; D006241:Haptens; D006801:Humans; D007106:Immune Sera / Q000032:analysis / Q000276:immunology; D007114:Immunization; D008297:Male; D011786:Quality Control; D011863:Radioimmunoassay / Q000379:methods*; D012016:Reference Values; D012710:Serum Albumin, Bovine / Q000276:immunology; D014316:Tritium
D000732:Androstanols; D006241:Haptens; D007106:Immune Sera; D014316:Tritium; D015113:Androstane-3,17-diol; C028895:androstane-3,17-diol glucuronide; D012710:Serum Albumin, Bovine