Sandwich ELISA assay for quantitative measurement of SP-40,40 in seminal plasma and serum.
SP-40,40 was purified from human plasma by PEG fractionation, DEAE-Sephacel, Phenyl-Toyopearl 650M, Bio-Gel A-0.5m and hydroxylapatite chromatographies. Three monoclonal antibodies (IF12, IID9 and IVF4) to this protein were prepared: IF12 and IID9 were specific for the beta subunit and IVF4 for the alpha subunit. The concentrations of SP-40,40 in seminal plasmas and sera were determined using a sandwich ELISA method. The results showed that the average concentrations of SP-40,40 were 438 +/- 285 micrograms/ml in seminal plasmas and 111 +/- 50 micrograms/ml in sera of normal donors. SP-40,40 concentrations in seminal plasmas of Klinefelter and excretory azoospermia patients were similar to those of normal donors. However, those of oligozoospermia and idiopathic azoospermia patients were about half the normal value.
Choi
N H
NH
Department of Physiological Chemistry, School of Pharmaceutical Sciences, Tokyo, Japan.
Tobe
T
T
Hara
K
K
Yoshida
H
H
Tomita
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D001798:Blood Proteins / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D051152:Clusterin; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018832:Molecular Chaperones; D009845:Oligospermia / Q000378:metabolism; D012661:Semen / Q000032:analysis*
D000911:Antibodies, Monoclonal; D001798:Blood Proteins; C495242:CLU protein, human; C495243:Clu protein, mouse; D051152:Clusterin; D006023:Glycoproteins; D018832:Molecular Chaperones