Determination of cocaine concentrations in plasma by high-performance liquid chromatography.
We describe a procedure for measuring concentrations of cocaine in plasma by reversed-phase high-performance liquid chromatography, with ion-pairing. The procedure involves solvent extraction followed by back-extraction with dilute acid. The n-propyl ester of benzoylecgonine is used as an internal standard. An evaporation step is not required, and concentrations as low as 5 micrograms/L can be quantified. Plasma concentrations of cocaine determined by high-performance liquid chromatography correlated well with those determined by a previously reported gas-chromatographic procedure with nitrogen detection.
Jatlow
P
P
Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT 06510.
Nadim
H
H
D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D003042:Cocaine / Q000097:blood* / Q000592:standards; D004340:Drug Contamination; D006801:Humans; D007202:Indicators and Reagents
D007202:Indicators and Reagents; D003042:Cocaine