The 83-kilodalton antigen of Borrelia burgdorferi which stimulates immunoglobulin M (IgM) and IgG responses in infected hosts is expressed by a chromosomal gene.
We report the cloning and characterization of a chromosomal gene from Borrelia burgdorferi expressing an 83-kilodalton protein antigen in Escherichia coli. The antigen reacted strongly with antisera from two human Lyme disease patients. The chromosomal gene was expressed in a 6.5-kilobase-pair ClaI fragment cloned into a variable-reading-frame plasmid vector.
Lefebvre
R B
RB
Department of Veterinary Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis 95616.
Perng
G C
GC
Johnson
R C
RC
D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000235:genetics*; D015748:Borrelia burgdorferi Group / Q000235:genetics / Q000276:immunology*; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D008193:Lyme Disease / Q000276:immunology*; D008970:Molecular Weight
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M