title: Determination of triethylenetetramine in plasma of patients by high-performance liquid chromatography.
abstract: A sensitive and simple fluorometric method for the determination of N,N'-bis(2-aminoethyl)-1,2-ethanediamine dihydrochloride (triethylenetetramine) in human plasma by high-performance liquid chromatography is described. Free triethylenetetramine (TETA) obtained by passing the TETA-copper chelate compound through a solid-phase cation exchange resin was converted to its fluorescamine derivative in the presence of ethylenediaminetetraacetic acid to mask the interfering metal ions in the reaction solution, and the derivatives were separated on a nitrile high-performance liquid chromatograph column (Nucleosil 5-CN) using isocratic elution. The plasma levels of TETA were measured in eight patients receiving treatment for excess copper. Absorption rates of TETA were relatively slow and the peak levels were significantly different among patients. The bioavailability of TETA in the rat was also examined and the ratio of intestinal absorption was extremely low.
authors.affiliation: Department of Pharmacy, Hokkaido University Hospital, School of Medicine, Sappora, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Miyazaki
authors.forename: S
authors.initials: S
authors.lastname: Kishino
authors.forename: M
authors.initials: M
authors.lastname: Kobayashi
authors.forename: S
authors.initials: S
authors.lastname: Arashima
authors.forename: S
authors.initials: S
authors.lastname: Matsumoto
authors.forename: T
authors.initials: T
authors.lastname: Arita
mesh_terms: D000818:Animals; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid; D005029:Ethylenediamines / Q000097:blood*; D006801:Humans; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D014266:Trientine / Q000097:blood*
chemical_list: D005029:Ethylenediamines; D014266:Trientine