title: [Obtaining of mutants of Streptomyces fradiae producing tylosin by action of nitrosomethylurea on synchronously germinating spores].
abstract: The method of localized mutagenesis was applied to obtain mutants of Streptomyces fradiae producing higher amounts of tylosin. The populations of the germinating spores were subjected to a short-term treatment with nitrosomethylurea during different periods of the first DNA replication cycle. The method reveals defines periods sensitive to mutation induction and isolates a mutant producing a 60% increase in the yield of tylosin as compared to that provided by the stock strain.
authors.forename: Z I
authors.initials: ZI
authors.lastname: Panfilova
authors.forename: N V
authors.initials: NV
authors.lastname: Dubrovina
authors.forename: N A
authors.initials: NA
authors.lastname: Tomashevskaia
mesh_terms: D004261:DNA Replication; D004269:DNA, Bacterial / Q000032:analysis; D008770:Methylnitrosourea / Q000494:pharmacology*; D009154:Mutation; D013171:Spores, Bacterial; D013302:Streptomyces / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D013997:Time Factors; D015645:Tylosin / Q000096:biosynthesis*
chemical_list: D004269:DNA, Bacterial; D008770:Methylnitrosourea; D015645:Tylosin
vernacular_title: Poluchenie mutantov Streptomyces fradiae, produtsentov tilozina, vozdeĭstviem nitrozometilmocheviny na sinkhronno-prorastoiushchie spory.