Localization of aldolase C mRNA in brain cells.
The expression of aldolase C and aldolase A mRNA was assessed by Northern blot hybridization using RNAs purified from cultured rat and mouse brain neurons and astroglial cells. Neurons were found to contain about 4-fold more aldolase C mRNA and about twice as much aldolase A mRNA than astroglia. Analysis of the cellular localization of aldolase C mRNA by in situ hybridization to brain slices showed a predominantly neuronal labeling with an irregular distribution. A strong signal was observed in Purkinje cell somata and a weaker signal in subpopulations of neurons in cerebral cortex, striatum, hippocampus, hypothalamic nuclei and primary olfactory cortex.
Popovici
T
T
INSERM U129, Paris, France.
Berwald-Netter
Y
Y
Vibert
M
M
Kahn
A
A
Skala
H
H
D000818:Animals; D001253:Astrocytes / Q000201:enzymology; D015152:Blotting, Northern; D001921:Brain / Q000166:cytology / Q000201:enzymology*; D001931:Brain Mapping; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D009474:Neurons / Q000201:enzymology; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D012333:RNA, Messenger; D005634:Fructose-Bisphosphate Aldolase