[Reproducibility of differential bronchoalveolar lavage cytology in sarcoidosis in relation to elapsed time between bronchoalveolar lavage and sample processing].
The physiological saline solution that is usually employed for BAL is not suitable as a storage medium for BAL cells for any longer period of time: even when cooled to 4 degrees C, the lymphocyte component decreases significantly after a latency period of 24 hours. With respect to the differential cytology performed on BAL fluid, the tissue culture medium RPMI 1640 + 5% AB serum can be employed as a storage medium. But even with this medium, a very clear reduction in the total cell count in BAl is seen at 24 hours.
Steppling
H
H
Universitätskliniken Mainz, III. Medizinische Klinik-Schwerpunkt Pneumologie.
Mühlburger
M
M
Müller-Quernheim
J
J
Hürter
T
T
D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology*; D006801:Humans; D007958:Leukocyte Count; D008171:Lung Diseases / Q000175:diagnosis*; D008264:Macrophages / Q000166:cytology; D011650:Pulmonary Alveoli / Q000166:cytology; D015203:Reproducibility of Results; D012507:Sarcoidosis / Q000175:diagnosis*; D013048:Specimen Handling / Q000379:methods*; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods
Untersuchungen über die Reproduzierbarkeit der BAL-Differentialzytologie bei Sarkoidose in Abhängigkeit vom Zeitabstand zwischen BAL und Materialaufarbeitung.