Survival of maternal mRNA in anucleate and unfertilized mouse eggs.
Unfertilized mouse eggs were parthenogenetically activated in vitro and then bisected. Anucleate fragments were aged in vitro, and their protein synthesis was analyzed by two-dimensional polyacrylamide gel electrophoresis. Proteins were compared with those which were synthesized by aging unfertilized eggs and those which were translated in vitro from mRNA extracted from the unfertilized eggs. Normally cleaving parthenogenetic eggs served as controls. Cytoplasts and unfertilized eggs synthesized considerable quantities of protein after 2 days in culture. The protein patterns of cytoplasts and unfertilized eggs shifted in this time mainly within a group of proteins with a molecular mass of about 35 kDa. This shift was also seen in controls between day 1 and 2 but was delayed in unfertilized eggs. There was no clear appearance of new proteins in aging cytoplasts, which might have indicated a selective activation of maternal mRNA at a certain time after the activation stimulus, nor was such a change apparent in unfertilized eggs. The survival of maternal allozymes of glucose phosphate isomerase was tested in cytoplasts derived from fertilized eggs. The allozymes remained active during 4 days of aging and did not change their quantitative correlation.
Petzoldt
U
U
Department of Biology, Philipps University, Marburg, Federal Republic of Germany.
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005956:Glucose-6-Phosphate Isomerase / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010063:Ovum / Q000378:metabolism*; D010312:Parthenogenesis; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D015053:Zygote / Q000378:metabolism*
D012333:RNA, Messenger; D005956:Glucose-6-Phosphate Isomerase