Perfusing pipettes.
A simple modification of a patch-clamp set-up allows the fluid in a patch pipette to be changed. The time course of the solution exchange is estimated from the time course of change of the reversal potential of the current through an open Ca2+ activated K+ channel: solution exchange takes less than 1 min. Measurements of the power spectrum of noise show that the modified set-up introduces little excess noise below 1 kHz.
Tang
J M
JM
Department of Physiology, Rush Medical College, Chicago, IL 60612.
Wang
J
J
Quandt
F N
FN
Eisenberg
R S
RS
D004594:Electrophysiology / Q000295:instrumentation* / Q000379:methods; D010477:Perfusion / Q000295:instrumentation* / Q000379:methods