Conditioned enhancement of skin allografts in mice.
Healthy A/J mice grafted with either BALB/c or C57BL/6 tail skin routinely reject these grafts with a mean survival time (MST) of 12-14 days. Low dose cyclophosphamide, Cy (50 mg/kg) on the day of engraftment can enhance survival of both grafts (MST 17-20 days). If mice are given three weekly intravenous injections of BALB/c peripheral blood prior to grafting, specific enhancement of BALB/c but not C57BL/6 grafts results (MST 18 and 12 days, respectively). Mice given several ip treatments with Cy in association with a novel taste (saccharin, Sacc) in their drinking water also show a conditioned immunosuppression if subsequently exposed to Sacc alone. Such mice given BALB/c or C57BL/6 skin grafts and re-exposed to Sacc also show prolonged survival of these skin allografts (MST 16-17 days). If conditioned mice are also treated, by pretransplant donor-specific transfusion, to develop a state of specific suppression of allograft immunity, then subsequent grafting with BALB/c or C57BL/6 grafts coupled with re-exposure to Sacc lead to a further prolonged survival of grafts specifically in the BALB/c mice (MST 29 days).
Gorczynski
R M
RM
Department of Surgery, University of Toronto, Ontario, Canada.
D000818:Animals; D001803:Blood Transfusion; D003213:Conditioning, Psychological / Q000502:physiology*; D003520:Cyclophosphamide / Q000494:pharmacology; D005260:Female; D006085:Graft Survival / Q000187:drug effects / Q000276:immunology*; D007116:Immunization, Passive; D007165:Immunosuppression Therapy; D051379:Mice; D008815:Mice, Inbred Strains; D011598:Psychoneuroimmunology; D012439:Saccharin; D016038:Skin Transplantation / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013649:Taste; D014184:Transplantation, Homologous
D003520:Cyclophosphamide; D012439:Saccharin