[Obtaining of mutants of Streptomyces fradiae producing tylosin by action of nitrosomethylurea on synchronously germinating spores].
The method of localized mutagenesis was applied to obtain mutants of Streptomyces fradiae producing higher amounts of tylosin. The populations of the germinating spores were subjected to a short-term treatment with nitrosomethylurea during different periods of the first DNA replication cycle. The method reveals defines periods sensitive to mutation induction and isolates a mutant producing a 60% increase in the yield of tylosin as compared to that provided by the stock strain.
Panfilova
Z I
ZI
Dubrovina
N V
NV
Tomashevskaia
N A
NA
D004261:DNA Replication; D004269:DNA, Bacterial / Q000032:analysis; D008770:Methylnitrosourea / Q000494:pharmacology*; D009154:Mutation; D013171:Spores, Bacterial; D013302:Streptomyces / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D013997:Time Factors; D015645:Tylosin / Q000096:biosynthesis*
D004269:DNA, Bacterial; D008770:Methylnitrosourea; D015645:Tylosin
Poluchenie mutantov Streptomyces fradiae, produtsentov tilozina, vozdeĭstviem nitrozometilmocheviny na sinkhronno-prorastoiushchie spory.