Characterization of the Renibacterium salmoninarum haemagglutinin.
Water-extracted proteins from nine geographically diverse strains of Renibacterium salmoninarum, all of which agglutinated rabbit erythrocytes and rainbow trout spermatozoa, were compared by SDS-PAGE. Extracts from eight strains, including the type strain, ATCC 33209, were similar, containing a major protein of 57 kDa and a minor protein of 58 kDa. The SDS-PAGE protein profile of the Char strain did not contain the 58 kDa protein. A non-agglutinating strain, MT-239, which was also non-hydrophobic, did not produce any water-extractable protein. Immunoblot reactions with rabbit antiserum prepared against whole cells of the type strain demonstrated that the water-extracted haemagglutinins from the various strains were antigenically related. When purified by polyacrylamide gel zone electrophoresis, the haemagglutinin from R. salmoninarum ATCC 33209 formed a doublet band with molecular masses of 57 and 58 kDa, similar to the previously described F antigen. The water-extracted haemagglutinin agglutinated salmonid spermatozoa, was degraded by protease K and trypsin, and was shown to self-assemble onto the cell surface.
Daly
J G
JG
Department of Microbiology, College of Biological Sciences, University of Guelph, Ontario, Canada.
Stevenson
R M
RM
D000818:Animals; D000942:Antigens, Bacterial / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005393:Fish Diseases / Q000382:microbiology; D006094:Gram-Positive Bacteria / Q000032:analysis* / Q000276:immunology; D006388:Hemagglutinins / Q000302:isolation & purification*; D015151:Immunoblotting; D007674:Kidney Diseases / Q000382:microbiology / Q000662:veterinary; D008970:Molecular Weight; D012487:Salmonidae
D000942:Antigens, Bacterial; D006388:Hemagglutinins