title: The protective effects of cell-free fluid exudate on cartilage degradation in vitro.
abstract: In a short-term culture system, polymorphonuclear neutrophils (PMNs) were shown to cause significant loss of glycosaminoglycan (GAG) from cartilage. In addition, a lysate of these cells, and in particular cells stimulated with a phorbol ester, greatly enhanced this loss of GAG. This loss could be partially or completely inhibited by the addition of cell-free fluid exudate to the system. In other long-term culture experiments, IL-1a was shown to enhance fibroblast-induced GAG loss from cartilage. In this system too the breakdown was inhibited by cell-free fluid exudate. Initial characterization of the protective agent or agents shows them to be heat-stable at 56 degrees C and to be non-dialysable. It is proposed that in the chronic inflammatory joint disease, rheumatoid arthritis, the fluid phase of an inflammatory response may have a protective effect against the underlying pathological processes.
authors.affiliation: Department of Experimental Pathology, St. Bartholomew's Hospital Medical College, London, England.
authors.forename: A R
authors.initials: AR
authors.lastname: Moore
authors.forename: F M
authors.initials: FM
authors.lastname: Desa
authors.forename: T H
authors.initials: TH
authors.lastname: Hanahoe
authors.forename: P R
authors.initials: PR
authors.lastname: Colville-Nash
authors.forename: C L
authors.initials: CL
authors.lastname: Chander
authors.forename: D W
authors.initials: DW
authors.lastname: Howat
authors.forename: D A
authors.initials: DA
authors.lastname: Willoughby
mesh_terms: D000818:Animals; D001172:Arthritis, Rheumatoid / Q000378:metabolism; D002356:Cartilage / Q000378:metabolism* / Q000473:pathology; D002474:Cell-Free System / Q000378:metabolism; D005122:Exudates and Transudates / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism*; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000494:pharmacology; D008297:Male; D009504:Neutrophils / Q000378:metabolism*; D051381:Rats
chemical_list: D006025:Glycosaminoglycans; D007375:Interleukin-1