Application of luminol-dependent chemiluminescence to assay opsonizing antibodies to procyclic forms of Trypanosoma congolense in the sera of dogs experimentally infected with heterologous stocks.
Luminol-dependent chemiluminescence (LCL) responses of dog granulocytes were used to assay opsonizing antibodies to procyclic culture forms of T. congolense. A high degree of sensitivity was demonstrated and LCL levels were high, when the phagocytic cells were incubated with the sera of infected dogs even at dilutions as high as 1:400 as compared to pre-infection or negative sera. The levels of opsonizing antibodies were elevated in all the dogs throughout the time of observation. The technique was sensitive, could be automated and, therefore, allows a rapid evaluation of large numbers of serum samples for Trypanosoma specific antibodies.
Olaho-Mukani
W
W
Institut für Parasitologie und Tropenveterinärmedizin, Freie Universität Berlin.
Winter
P
P
Dörflinger
W
W
Hörchner
F
F
Ahmed
J S
JS
D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis*; D004285:Dogs; D006098:Granulocytes / Q000276:immunology*; D008163:Luminescent Measurements; D008165:Luminol; D009895:Opsonin Proteins / Q000032:analysis; D014348:Trypanosoma congolense / Q000276:immunology*; D014353:Trypanosomiasis, African / Q000276:immunology*
D000913:Antibodies, Protozoan; D009895:Opsonin Proteins; D008165:Luminol