[Changes in the content of cytochrome P-450 and the activity of microsomal and cytosolic glutathione transferases in the liver of guinea pigs undergoing cutaneous application of various doses of mineral oil made from petroleum containing polycyclic aromatic hydrocarbons].
Guinea pigs received skin application of petroleum-derived mineral oil distillate, containing about 10% of polycyclic aromatic hydrocarbons with the 4 hours exposure time everyday during 20 days. 100% distillate preparation and it's 50%, 3% or 0.5% solutions in furfurol and ethanol are used. It resulted in the distillate-dose-dependent cytochrome P-450 induction (1.38-2.23-fold) in liver of the all exposed groups of guinea pigs and in 20-60% decrease in microsomal and cytosol glutathione transferase activities in groups which received 50% and 3% mineral oil distillate solutions. Ratio values of cytochrome P-450 content level to glutathiontransferase activity level depended linearly on the distillate doses, and it increased 2.7-4.4-fold with the distillate concentration increasing in the preparation from 0.5% to 50%. Conclusion was made that with increasing distillate doses the process of polycyclic aromatic hydrocarbon activation with the genotoxic metabolite formation predominated over the process of those metabolite detoxication.
Kravchenko
M N
MN
Ausheva
L Kh
LKh
Bendikov
E A
EA
D000279:Administration, Cutaneous; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis* / Q000096:biosynthesis; D003600:Cytosol / Q000187:drug effects* / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction / Q000187:drug effects; D005982:Glutathione Transferase / Q000032:analysis* / Q000096:biosynthesis; D006168:Guinea Pigs; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology; D008899:Mineral Oil / Q000008:administration & dosage*; D011083:Polycyclic Compounds / Q000008:administration & dosage*; D013997:Time Factors
D011083:Polycyclic Compounds; D008899:Mineral Oil; D003577:Cytochrome P-450 Enzyme System; D005982:Glutathione Transferase
Izmeneniia soderzhaniia tsitokhroma P-450 i aktivnosti mikrosomal'noĭ i tsitozol'noĭ glutationtransferaz v pecheni morskikh svinok pri nakozhnoĭ applikatsii razlichnykh doz mineral'nogo masla iz nefti, soderzhashchego politsiklicheskie aromaticheskie uglevodorody.