Measurement of free human leukocyte elastase and human leukocyte elastase/alpha 1 proteinase inhibitor complexes by an enzyme-linked immunosorbent assay.
We report on an ELISA procedure for the quantitative analysis of total human leukocyte (PMN)-elastase, i.e., the simultaneous determination of (i) free (non-complexed) PMN-elastase and of (ii) PMN-elastase that is complexed to alpha 1 proteinase inhibitor. Simultaneous detection of both forms of PMN-elastase was achieved using a monoclonal antibody that recognizes a PMN-elastase-specific epitope present on both the uncomplexed and the complexed form of the enzyme. The test system described is reliable, easy to perform and permits the determination of total PMN-elastase in complex biological fluids such as plasma or seminal fluid. It is to be expected that this test system will be useful for investigations of human PMN-elastase in biological specimens obtained from both normal and pathological conditions.
Kramer
M D
MD
Universitäts-Hautklinik, Heidelberg, F.R.G.
Müller-Bardorff
M
M
Simon
M M
MM
Tilgen
W
W
Schickel
E
E
Petzoldt
D
D
D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007962:Leukocytes / Q000201:enzymology*; D046911:Macromolecular Substances; D010196:Pancreatic Elastase / Q000032:analysis* / Q000276:immunology; D012661:Semen / Q000201:enzymology; D000515:alpha 1-Antitrypsin / Q000032:analysis*
D000911:Antibodies, Monoclonal; D046911:Macromolecular Substances; D000515:alpha 1-Antitrypsin; D010196:Pancreatic Elastase