Induction of host resistance to Rhipicephalus appendiculatus in rabbits: effects of immunizing with detergent-solubilized tick tissue proteins.
Resistance to the hard tick, Rhipicephalus appendiculatus, was induced in rabbits by immunizing them with tick tissue proteins extracted with a detergent, Triton X-100. There was 25% mortality in female ticks fed on immunized rabbits as compared with those fed on controls. Similarly, there was a 40% and 60% reduction in the engorged weight and the weight of egg batches, respectively, of ticks fed on immunized rabbits. Western blot analysis of detergent-solubilized tick tissue proteins, carried out using immune sera, recognized a complex pattern of proteins. A strong reaction was observed with proteins with apparent molecular weights of 94,000 and 40,000 daltons.
Dhadialla
T S
TS
International Centre of Insect Physiology and Ecology, Nairobi, Kenya.
Rutti
B
B
Brossard
M
M
D000818:Animals; D000941:Antigens / Q000032:analysis / Q000276:immunology; D001096:Arachnid Vectors / Q000276:immunology*; D005247:Feeding Behavior; D005260:Female; D007114:Immunization; D015151:Immunoblotting; D008297:Male; D010058:Oviposition; D011817:Rabbits; D013984:Tick Infestations / Q000276:immunology*; D013987:Ticks / Q000276:immunology* / Q000502:physiology
D000941:Antigens