title: In situ hybridization analysis of isodicentric X-chromosomes with short arm fusion.
abstract: We present here an alternative approach to the study of mosaic cell lines containing dicentric chromosomes. The approach is based on chromosome-specific non-radioactive in situ hybridization with centromere (alpha satellite DNA) probes. The hybridization analysis may be used as an alternative to the C-band analysis, while at the same time to some extent replacing the Q-band analysis as well. The advantage of using in situ hybridization is mainly that it allows the very fast screening of a large number of metaphases. We illustrate this new application of the technique by using it for the analysis of two cases of isodicentric X-chromosomes. The approach is expected to be generally applicable, so that it may be applied to the scoring of other types of chromosomal mosaicism as well.
authors.affiliation: Institute of Human Genetics, University of Aarhus, Denmark.
authors.forename: J E
authors.initials: JE
authors.lastname: Koch
authors.forename: S
authors.initials: S
authors.lastname: Kølvraa
authors.forename: J M
authors.initials: JM
authors.lastname: Hertz
authors.forename: K
authors.initials: K
authors.lastname: Rasmussen
authors.forename: N
authors.initials: N
authors.lastname: Gregersen
authors.forename: G F
authors.initials: GF
authors.lastname: Fly
authors.forename: L A
authors.initials: LA
authors.lastname: Bolund
mesh_terms: D002648:Child; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002874:Chromosome Mapping; D003582:Cytogenetics / Q000379:methods* / Q000592:standards; D015342:DNA Probes; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D009030:Mosaicism / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D014960:X Chromosome / Q000648:ultrastructure*
chemical_list: D015342:DNA Probes