title: Replacement of threonine residues by serine and alanine in a phosphorylatable heavy chain fragment of Dictyostelium myosin II.
abstract: The target sites of soluble myosin heavy chain kinases partially purified from growth phase or aggregation competent cells of Dictyostelium discoideum were identified by the use of normal and mutated fragments of the myosin heavy chain. The kinases from both developmental stages phosphorylated two previously established threonine residues, as well as an additional one. The newly identified site is located within the putative core region of the coiled-coil formed by the myosin tail. A lysine following the phosphorylated threonine residue is the only common feature of the sequences around these sites. The kinases, which specifically phosphorylate threonine residues in wild-type myosin, did accept serine if it was in the right structural context.
authors.affiliation: Max-Planck-Institut für Biochemie, Martinsried, FRG.
authors.forename: D
authors.initials: D
authors.lastname: Lück-Vielmetter
authors.forename: M
authors.initials: M
authors.lastname: Schleicher
authors.forename: B
authors.initials: B
authors.lastname: Grabatin
authors.forename: J
authors.initials: J
authors.lastname: Wippler
authors.forename: G
authors.initials: G
authors.lastname: Gerisch
mesh_terms: D000409:Alanine / Q000378:metabolism; D000595:Amino Acid Sequence; D004252:DNA Mutational Analysis; D004023:Dictyostelium / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism*; D009218:Myosins / Q000235:genetics / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism; D010766:Phosphorylation; D010768:Phosphoserine / Q000378:metabolism; D010769:Phosphothreonine / Q000378:metabolism; D015183:Restriction Mapping; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
chemical_list: D010446:Peptide Fragments; D010769:Phosphothreonine; D010768:Phosphoserine; D009219:Myosin-Light-Chain Kinase; D009218:Myosins; D000409:Alanine