The formation of asbestos bodies by mouse peritoneal macrophages. An in vitro study.
For studies on the mechanism of asbestos body formation, Union Internationale Contre Cancer (UICC) crocidolite asbestos fibers were added to a culture of mouse peritoneal macrophages. Small asbestos fibers were totally ingested by the macrophages, but fibers too long to be taken up completely remained as a consequence extracellular. These long asbestos fibers became the basis for asbestos body formation. The basic mechanism underlying asbestos body formation was found to be the exocytotic activity of macrophages. The number of iron-rich inclusion bodies was dependent on the availability of iron in the culture media, and the same holds for the amount of iron in the asbestos body coat. This means that asbestos body formation is a phenomenon that occurs accidentally when macrophages come into contact with long fibers in an iron-rich environment. A time-dependent increase in the number, average size, and rate of segmentation of the asbestos bodies was observed. The present report is the first to describe asbestos body formation in vitro.
Koerten
H K
HK
Laboratory for Electron Microscopy, University of Leiden, The Netherlands.
de Bruijn
J D
JD
Daems
W T
WT
D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D001194:Asbestos / Q000378:metabolism*; D002449:Cell Aggregation; D002478:Cells, Cultured; D004705:Endocytosis; D006046:Gold / Q000378:metabolism; D007501:Iron / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D051379:Mice
D001194:Asbestos; D006046:Gold; D007501:Iron; D000135:Acid Phosphatase