Rapid purification and characterization of histatins (histidine-rich polypeptides) from human whole saliva.
Three different polypeptides capable of stimulating histamine release from mast cells were isolated from human whole saliva, using heparin-gel chromatography followed by reversed-phase HPLC. The amino acid sequences of these peptides were shown to be identical to those of histatins 1, 3 and 5.
Sugiyama
K
K
Department of Pharmacology, Okayama University Dental School, Japan.
Ogino
T
T
Ogata
K
K
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006023:Glycoproteins / Q000032:analysis / Q000302:isolation & purification*; D006632:Histamine / Q000032:analysis; D006801:Humans; D008297:Male; D008407:Mast Cells / Q000032:analysis; D008969:Molecular Sequence Data; D011506:Proteins / Q000032:analysis / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains; D012463:Saliva / Q000032:analysis*; D012471:Salivary Proteins and Peptides / Q000032:analysis / Q000302:isolation & purification*; D000099053:Histidine-Rich Glycoprotein
D000596:Amino Acids; D006023:Glycoproteins; D011506:Proteins; D012471:Salivary Proteins and Peptides; D000099053:Histidine-Rich Glycoprotein; D006632:Histamine