Evidence that the action of clonixin is non-dependent of ACh release.
1. The present study was designed to verify the ACh-mediated role in the action of ClX using cardiovascular and non-cardiovascular tissues taken from rats. 2. In the vasa deferentia preparations the muscular twitch induced by TNS was not changed by atropine, however in the presence of this drug, ClX induced a significant reduction of the height of the TNS-evoked twitch. 3. In the cardiorespiratory experiments, the administration of ClX produced a dose-dependent decrease in MAP, HR and RF. These effects were not changed by bilateral vagotomy and cervical sympathectomy. 4. The results obtained with atropine and with bilateral vagotomy and sympathectomy, attempt to delineate the possibility of a direct action of ClX. 5. All the evidence taken together does not support the hypothesis that ClX may be acting through an indirect action by releasing ACh.
Saavedra
H
H
Department of Pharmacology, School of Medicine, Faculty of Medicine, Universidad de Chile, Santiago.
Miranda
H F
HF
Bustamante
D
D
Pelissier
T
T
Paeile
C
C
D000109:Acetylcholine / Q000502:physiology*; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001794:Blood Pressure / Q000187:drug effects; D003002:Clonixin / Q000494:pharmacology*; D005260:Female; D006339:Heart Rate / Q000187:drug effects; D006439:Hemodynamics / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D009539:Nicotinic Acids / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012119:Respiration / Q000187:drug effects; D013562:Sympathectomy; D014628:Vagotomy; D014649:Vas Deferens / Q000187:drug effects
D009539:Nicotinic Acids; D001285:Atropine; D000109:Acetylcholine; D003002:Clonixin