Protein kinase regulates tumor necrosis factor mRNA stability in virus-stimulated astrocytes.
Infection of astrocytes with Newcastle disease virus stimulated the production of 1,2-diacylglycerol, and resulted in the kinase-dependent expression of mRNAs encoding tumor necrosis factor (TNF), interferon alpha and beta, and interleukin 6. The half-life of TNF mRNA was significantly decreased in the presence of protein kinase inhibitors H-7 and staurosporine, but not in the presence of HA1004. In contrast to the decay of TNF mRNA, the half-lives of other cytokine mRNAs were only minimally affected by the kinase inhibitors. These data indicated that the stability of TNF mRNA was regulated through a novel, kinase-dependent pathway.
Lieberman
A P
AP
Department of Pathology, University of Maryland School of Medicine, Baltimore 21201.
Pitha
P M
PM
Shin
M L
ML
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000470:Alkaloids / Q000494:pharmacology; D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D015152:Blotting, Northern; D002467:Cell Nucleus / Q000378:metabolism; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004075:Diglycerides / Q000378:metabolism; D007546:Isoquinolines / Q000494:pharmacology; D009522:Newcastle disease virus / Q000235:genetics*; D010879:Piperazines / Q000494:pharmacology; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D019311:Staurosporine; D013449:Sulfonamides; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D000470:Alkaloids; D000546:Amanitins; D004075:Diglycerides; D007546:Isoquinolines; D010879:Piperazines; D047428:Protein Kinase Inhibitors; D012333:RNA, Messenger; D013449:Sulfonamides; D014409:Tumor Necrosis Factor-alpha; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; C043204:N-(2-guanidinoethyl)-5-isoquinolinesulfonamide; D011494:Protein Kinases; D019311:Staurosporine