Beneficial effect of tan-shen, an extract from the root of Salvia, on post-hypoxic recovery of cardiac contractile force.
The present study was undertaken to elucidate the possible effects of tanshinone VI, one of the extracts from the root of Salvia, on post-hypoxic recovery of cardiac contractile force. For this purpose, rat hearts were perfused for 45 min under reoxygenated conditions following 20-min hypoxic perfusion, and changes in tissue high-energy phosphates and calcium contents, and release of ATP metabolites and creatine kinase were examined. Post-hypoxic recovery of cardiac contractile force was augmented when hearts were treated with 42 nM tanshinone VI during hypoxia. This beneficial recovery was accompanied by enhanced restoration of myocardial high-energy phosphates, depression of hypoxia- and reoxygenation-induced increase in tissue calcium content, and suppression of release of ATP metabolites such as adenosine, inosine and hypoxanthine from the perfused heart. The results suggest that tanshinone VI is beneficial for the recovery of cardiac contractility after a certain period of oxygen-deficiency, possibly through mechanisms involving improvement of myocardial energy production upon oxygen-replenishment and/or inhibition of calcium accumulation in the cardiac cell.
Takeo
S
S
Department of Pharmacology, Tokyo College of Pharmacy, Japan.
Tanonaka
K
K
Hirai
K
K
Kawaguchi
K
K
Ogawa
M
M
Yagi
A
A
Fujimoto
K
K
D045784:Abietanes; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002317:Cardiovascular Agents / Q000378:metabolism / Q000627:therapeutic use*; D003402:Creatine Kinase / Q000378:metabolism; D000860:Hypoxia / Q000188:drug therapy*; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects*; D015425:Myocardial Reperfusion; D010616:Phenanthrenes / Q000627:therapeutic use*; D010710:Phosphates / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D045784:Abietanes; D002317:Cardiovascular Agents; D010616:Phenanthrenes; D010710:Phosphates; C021751:tanshinone; D000255:Adenosine Triphosphate; D003402:Creatine Kinase; D002118:Calcium