Prolactin directly stimulates citrate production and mitochondrial aspartate aminotransferase of prostate epithelial cells.
Prolactin, in vitro, significantly increased citrate production, mAAT (mitochondrial aspartate aminotransferase) and pmAAT (precursor form of mAAT) activity of prostate epithelial cells derived from rat lateral prostate (LP) and pig prostate cultures. In contrast, prolactin had no effect on the cytosolic isozyme, cAAT. This prolactin effect appeared to be independent of testosterone. The phorbol ester TPA (12-O-tetradecanoyl-phorbol-13-acetate) induced the same effects as prolactin thereby indicating the involvement of protein kinase C. This report demonstrates that prolactin directly regulates citrate production of prostate epithelial cells and the availability of an in vitro model to elucidate the mechanism of action of prolactin.
Franklin
R B
RB
Department of Physiology, Dental School, University of Maryland, Baltimore 21201.
Costello
L C
LC
D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism*; D002478:Cells, Cultured; D002951:Citrates / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D004848:Epithelium / Q000187:drug effects / Q000201:enzymology; D008297:Male; D008928:Mitochondria / Q000187:drug effects* / Q000201:enzymology; D010703:Phorbol Esters / Q000494:pharmacology; D011388:Prolactin / Q000494:pharmacology*; D011467:Prostate / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D013552:Swine
D002951:Citrates; D010703:Phorbol Esters; D011388:Prolactin; D001219:Aspartate Aminotransferases; D011493:Protein Kinase C