[Mechanism of the photodamage of eye structures. Changes in the lens crystalline charges after ultraviolet irradiation].
UV photodamaging action on individual soluble proteins of the cattle eye crystalline lens were studied by electrofocusing. The most quantitative and qualitative changes were found in gamma-crystallines: a decrease of original polypeptide stripes and appearance of additional components in pI 5.1-6.4 region. The illumination of alpha- and beta H-crystallines resulted in qualitative changes. The appearance of aggregates with the molecular mass above 10(6)D were noticed in all the protein fractions, except beta H-crystalline.
El'chaninov
V V
VV
Fedorovich
I B
IB
D000818:Animals; D002417:Cattle; D003459:Crystallins / Q000032:analysis / Q000528:radiation effects*; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D007525:Isoelectric Focusing; D007908:Lens, Crystalline / Q000528:radiation effects*; D010455:Peptides / Q000032:analysis / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects*
D003459:Crystallins; D010455:Peptides
Mekhanizm fotopovrezhdeniia struktur glaza. Izmenenie zariadov kristallinov khrustalika pri ul'trafioletovom obluchenii.