Damage to red blood cells by halocompounds.
Twenty-five halocompounds were screened as inducers of hemoglobin degradation and lipid peroxidation in rat red blood cells (RBC). RBC activated halocompounds, and oxyhemoglobin remaining depended on the halocompounds employed. Thiobarbituric-acid-reactive substances (TBARS), an index of lipid peroxidation, also depended on the halocompound. TBARS were inversely correlated and oxyhemoglobin percentage was directly correlated with the logarithm of oral LD50 of the halocompounds (r = 0.79, P less than 0.001, and r = 0.87, P less than 0.001, respectively). For 15 halocompounds, the oral LD50 calculated as log LD50 = 0.015 (% hemoglobin) -0.05 (TBARS) were correlated (r = 0.88, P less than 0.01) with the reported oral LD50.
Hidalgo
F J
FJ
Department of Food Science and Technology, University of California, Davis 95616.
Zamora
R
R
Tappel
A L
AL
D000818:Animals; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D006846:Hydrocarbons, Halogenated / Q000008:administration & dosage / Q000633:toxicity*; D007928:Lethal Dose 50; D008054:Lipid Peroxides / Q000096:biosynthesis; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D008433:Mathematics; D008706:Methemoglobin / Q000378:metabolism; D010108:Oxyhemoglobins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013858:Thiobarbiturates
D006846:Hydrocarbons, Halogenated; D008054:Lipid Peroxides; D010108:Oxyhemoglobins; D013858:Thiobarbiturates; D008315:Malondialdehyde; D008706:Methemoglobin; C029684:thiobarbituric acid