Eukaryotic tRNAs(Pro): primary structure of the anticodon loop; presence of 5-carbamoylmethyluridine or inosine as the first nucleoside of the anticodon.
The modified nucleoside U*, located in the first position of the anticodon of yeast, chicken liver and bovine liver tRNA(Pro) (anticodon U*GG), has been determined by means of TLC, HPLC, ultraviolet spectrum and gas chromatography-mass spectrometry. The structure was established as 5-carbamoylmethyluridine (ncm5U). In addition, we report on the primary structures of the above-mentioned tRNAs as well as those which have the IGG anticodon. In yeast, the two tRNA(Pro) (anticodons U*GG and IGG) differ by eight nucleotides, whereas in chicken and in bovine liver, both anticodons are carried by the same 'body tRNA' with one posttranscriptional exception at position 32, where pseudouridine is associated with ncm5U (position 34) in tRNA(Pro) (U*GG) and 2'-O-methylpseudouridine is associated with inosine (position 34) in tRNA(Pro) (IGG).
Keith
G
G
Institut de Biologie Moléculaire et Cellulaire du C.N.R.S. et de l'Université Louis Pasteur, Strasbourg, France.
Desgrès
J
J
Pochart
P
P
Heyman
T
T
Kuo
K C
KC
Gehrke
C W
CW
D000818:Animals; D000926:Anticodon / Q000032:analysis; D001483:Base Sequence; D002417:Cattle; D002645:Chickens; D002845:Chromatography; D007288:Inosine / Q000032:analysis*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D012331:RNA, Fungal / Q000235:genetics; D012343:RNA, Transfer / Q000032:analysis; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*; D012361:RNA, Transfer, Pro / Q000235:genetics*; D013056:Spectrophotometry, Ultraviolet; D014529:Uridine / Q000031:analogs & derivatives* / Q000032:analysis; D015003:Yeasts / Q000235:genetics
D000926:Anticodon; D012331:RNA, Fungal; D012345:RNA, Transfer, Amino Acid-Specific; D012361:RNA, Transfer, Pro; C030690:5-carbamoylmethyluridine; D007288:Inosine; D012343:RNA, Transfer; D014529:Uridine