title: On the measurement of particle number and mobility in nonideal solutions by fluorescence correlation spectroscopy.
abstract: Interparticle interactions are incorporated into the theoretical description of the initial amplitude, G(0), of the normalized fluorescence correlation spectroscopy autocorrelation function. Measurements of particle number, aggregate size, and interaction-dependent diffusion are then analyzed in the context of this generalized theory. It is shown that the neglect of interactions can introduce order-of-magnitude errors into estimates of particle number and aggregate size. It is also shown that measurement of G(0) provides an essentially unique method for testing the validity of theories of interaction-dependent membrane protein diffusion.
authors.affiliation: Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill 27599-7090.
authors.forename: J R
authors.initials: JR
authors.lastname: Abney
authors.forename: B A
authors.initials: BA
authors.lastname: Scalettar
authors.forename: C R
authors.initials: CR
authors.lastname: Hackenbrock
mesh_terms: D008433:Mathematics; D008565:Membrane Proteins; D008566:Membranes; D008954:Models, Biological; D009010:Monte Carlo Method; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D008565:Membrane Proteins