[Modeling of alpha-superhelical proteins. Selection of sequence, synthesis, and properties].
An analysis of the alpha-tropomyosin primary structure suggests a periodic sequence able to form an alpha-superhelical conformation. A repeating unit (the heptapeptide monomer) was obtained by sequential synthesis in solution using pentafluorophenyl esters of the N-protected amino acids. The polyheptapeptide (Glu-Lys-Lys-Leu-Glu-Glu-Ala)n with an average molecular weight 6500 has been synthesized by polymerization of the heptamer. It is shown that the polymer product forms a stable alpha-superhelix at acidic pH in water solution.
Potekhin
S A
SA
Iatsuk
O I
OI
Medvedkin
V N
VN
D000595:Amino Acid Sequence; D002855:Chromatography, Thin Layer; D002942:Circular Dichroism; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000032:analysis / Q000138:chemical synthesis; D011487:Protein Conformation; D014335:Tropomyosin / Q000032:analysis* / Q000138:chemical synthesis
D009842:Oligopeptides; D014335:Tropomyosin
Modelirovanie alpha-superspiral'nykh belkov. Vybor posledovatel'nosti, sintez i svoĭstva.