Fixation of a highly reactive form of alpha-chymotrypsin by micellar matrix.
Using reversed micelles of surfactants solvated by water-organic cosolvent mixtures as a matrix for enzyme entrapping, it is possible to fix the highly reactive alpha-chymotrypsin form. The reactivity of alpha-chymotrypsin towards nonspecific substrates increases to the extent comparable with that observed in reactions involving specific substrates.
Levashov
A V
AV
Department of Chemistry, M.V. Lomonosov Moscow State University, USSR.
Klyachko
N L
NL
Bogdanova
N G
NG
Martinek
K
K
D000818:Animals; D002417:Cattle; D002918:Chymotrypsin / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008823:Micelles; D009038:Motion; D010179:Pancreas / Q000201:enzymology; D011487:Protein Conformation; D012997:Solvents; D013329:Structure-Activity Relationship
D008823:Micelles; D012997:Solvents; D002918:Chymotrypsin