Identification of goat allotypic determinants and generation of goat-mouse hybridomas secreting goat IgG2.
Five allotypic determinants controlled by independent genes have been identified in goat. Of these determinants, four have been detected with alloimmune antisera and one with monoclonal antibodies. The specificities A1, C1 and D1 are lipoproteins; B1 is possibly an alpha 2 macroglobulin and E1 and IgG2. The specificity B1 is not expressed until the age of 3-4 months. The gene controlling the specificity E1 is present at about the same frequency (0.38-0.41) in goat, sheep, cattle and water buffalo. Stable hybridomas secreting goat IgG2 have been obtained by the fusion of goat peripheral lymphocytes with mouse myeloma cells.
Capparelli
R
R
Department of Animal Production, University of Naples, Portici, Italy.
Del Sorbo
G
G
Iannelli
D
D
D000818:Animals; D000911:Antibodies, Monoclonal; D002459:Cell Fusion; D015870:Gene Expression; D006041:Goats / Q000235:genetics* / Q000276:immunology; D006825:Hybridomas / Q000378:metabolism*; D007126:Immunoglobulin Allotypes / Q000032:analysis / Q000235:genetics*; D007074:Immunoglobulin G / Q000096:biosynthesis*; D051379:Mice / Q000235:genetics*; D011995:Recombination, Genetic
D000911:Antibodies, Monoclonal; D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G