[The effect of the method of detecting indocyanine green (HPLC, spectrophotometry) on the determination of pharmacokinetic parameters].
The method of determination of indocyanine green (ICG) in plasma by means of high-performance liquid chromatography (HPLC) is compared with the method of spectrophotometry. A sample for HPLC is purified by deproteination with acetonitrile and the supernatant is directly spread on the column. The internal standard is diazepam. The mobile phase consists of 50% of phosphate buffer pH 6 prepared according to PhBs 4, 47% of acetonitrile and 3% of methanol. Detection takes place at 260 nm. Spectrophotometric analysis consists in the measurement of plasma absorbance at 805 nm. Correlation of both methods is linear r = 0.989, n = 20, p less than 0.05. From the curves of elimination of the dye from the blood bed the principal pharmacokinetic parameters were calculated by means of a one-compartmental model after single intravascular administration. In spectrophotometric examination, elimination of ICG seems to be slower as compared with HPLC.
Aubrecht
J
J
Institut pro dalsí vzdĕlávání lékarů a farmaceutů, Praha.
Perlík
F
F
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007208:Indocyanine Green / Q000493:pharmacokinetics*; D008103:Liver Cirrhosis / Q000097:blood; D013053:Spectrophotometry
D007208:Indocyanine Green
Vliv metody detekce indocyaninové zelenĕ (HPLC, spektrofotometrie) na odhad farmakokinetických parametrů.