A54145, a new lipopeptide antibiotic complex: isolation and characterization.
A54145 is a complex of acidic lipopeptide antibiotics which are produced by Streptomyces fradiae and are active against Gram-positive bacteria. The A54145 complex was isolated by adsorption on Diaion HP-20 nonfunctionalized macroreticular resin and/or ion exchange on Amberlite IRA-68 anion exchange resin. Antibacterial factors A, A1, B, B1, C, D, E, and F were obtained in purified form by repeated preparative reverse phase HPLC on C8 and/or C18 bonded-phase supports. The molecular formulae of the factors are C72H109N17O27 (factors A and A1), C73H111N17O27 (factors B, B1, C, and D), C74H113N17O27 (factor E), and C71H107N17O27 (factor F). The identities of the acyl side chains were established as 8-methylnonanoyl (factors F, A, and B1), n-decanoyl (factors A1 and B), and 8-methyldecanoyl (factors C, D, and E).
Fukuda
D S
DS
Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285.
Du Bus
R H
RH
Baker
P J
PJ
Berry
D M
DM
Mynderse
J S
JS
D000327:Adsorption; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D008074:Lipoproteins / Q000032:analysis / Q000302:isolation & purification; D008969:Molecular Sequence Data; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet; D013302:Streptomyces / Q000378:metabolism
C065208:A54145; D000596:Amino Acids; D000900:Anti-Bacterial Agents; D008074:Lipoproteins