Anti-immunoglobulins induce death by apoptosis in WEHI-231 B lymphoma cells.
Anti-membrane immunoglobulin (anti-mIg) antibodies exert inhibitory effects in immature B lymphocytes such as WEHI-231 cells. We show here that anti-mIg treatment causes DNA fragmentation (apoptosis) in these cells. We also report that co-treatment with the protein kinase C activator phorbol 12-myristate 13-acetate prevents apoptosis induced by anti-mIg. These results are in agreement with our initial proposal that sensitivity to the toxic effects of anti-mIg reflects, at least partially, altered signal transduction in immature B lymphocytes. Variations in signal transduction pathways during B lymphocyte ontogeny may, therefore, play a critical role in determining whether B cells should be activated or inhibited via their mIg.
Benhamou
L E
LE
Département d'Immunologie, Institut Pasteur, Paris, France.
Cazenave
P A
PA
Sarthou
P
P
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002455:Cell Division / Q000276:immunology; D002470:Cell Survival / Q000276:immunology; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D007108:Immune Tolerance / Q000502:physiology; D051379:Mice; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D000888:Antibodies, Anti-Idiotypic; D004247:DNA; D013755:Tetradecanoylphorbol Acetate