title: Specific recognition of staphylococcal enterotoxin A by human T cells bearing receptors with the V gamma 9 region.
abstract: T cells bearing the alpha beta receptor can specifically react with target cells coated with staphylococcal enterotoxin and expressing major histocompatibility complex class II molecules; these responses depend on which variable region (V) of the receptor's beta-subunit is used. We have now examined whether a similar situation exists for human T cells bearing the gamma delta receptor. We found that reactivity to staphylococcal enterotoxin A is strictly dependent on the presence of the V gamma 9 variable region in the gamma delta T-cell receptor (TCR). These cytotoxic responses required the expression of HLA class II molecules by the target cell and could be inhibited by anti-gamma delta TCR and by anti-HLA-class-II monoclonal antibodies. In contrast to alpha beta TCR+ cell clones, no proliferative response of V gamma 9+ T-cell clones towards stimulator cells coated with enterotoxin A was observed in vitro. These results indicate that the gamma delta TCR repertoire might be influenced by enterotoxin A produced during staphylococcal infections in vivo. This could provide a molecular basis for the observation that V gamma 9+ T cells form the large majority of peripheral gamma delta TCR+ cells but only a small proportion of thymic gamma delta TCR+ cells.
authors.affiliation: Department of Immunohematology and Bloodbank, University Hospital, Leiden, The Netherlands.
authors.forename: C J
authors.initials: CJ
authors.lastname: Rust
authors.forename: F
authors.initials: F
authors.lastname: Verreck
authors.forename: H
authors.initials: H
authors.lastname: Vietor
authors.forename: F
authors.initials: F
authors.lastname: Koning
mesh_terms: D002460:Cell Line; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D004768:Enterotoxins / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D011948:Receptors, Antigen, T-Cell / Q000187:drug effects / Q000276:immunology*; D013211:Staphylococcus aureus; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D004768:Enterotoxins; D011948:Receptors, Antigen, T-Cell; C026066:enterotoxin A, Staphylococcal