title: Quantitation of endogenous liver apolipoprotein B mRNA editing.
abstract: The mRNA for apolipoprotein B is translated into either a high molecular weight (apo BH) or low molecular weight (apo BL) form of the protein depending on a novel form of RNA processing known as RNA editing. Apo BH mRNA editing is both tissue-specific and hormonally regulated and involves transition of cytidine to uridine at codon 2153 thereby converting a glutamine codon (CAA) to a translational stop codon (UAA). Three methods for quantitating the endogenous levels of liver apo B mRNA editing were compared: (1) Southern blot hybridization with discriminative thermal washes, (2) competimer-hybridization with discriminative thermal washes and (3) competimer-polymerase chain reaction (competimer-PCR). The data suggest that hybridization and PCR can yield similar quantitation when competing oligonucleotides are used. Based on competimer-PCR it is proposed that 40% and 85% of normal rat liver and small intestine apo B mRNA (respectively) are edited.
authors.affiliation: Department of Biochemistry, University of Rochester, NY 14642.
authors.forename: J W
authors.initials: JW
authors.lastname: Backus
authors.forename: M J
authors.initials: MJ
authors.lastname: Eagleton
authors.forename: S G
authors.initials: SG
authors.lastname: Harris
authors.forename: C E
authors.initials: CE
authors.lastname: Sparks
authors.forename: J D
authors.initials: JD
authors.lastname: Sparks
authors.forename: H C
authors.initials: HC
authors.lastname: Smith
mesh_terms: D000818:Animals; D001055:Apolipoproteins B / Q000235:genetics*; D001483:Base Sequence; D007421:Intestine, Small / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats
chemical_list: D001055:Apolipoproteins B; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger