Inhibition of connective tissue formation in dermal wounds covered with synthetic, moisture vapor-permeable dressings and its reversal by transforming growth factor-beta.
An investigation of synthetic, adherent, moisture vapor-permeable dressings (SAM) on dermal wounds healing by secondary intent has yielded the novel observation that SAM dressings severely inhibited the deposition of granulation tissue and subsequent collagenous tissue when compared with air-exposed wounds in mouse and guinea pig systems. Repair tissue was quantitated histomorphometrically in full-thickness wounds covered with SAM or left air-exposed for periods up to 3 weeks. Early in healing, mouse wounds left open to the atmosphere formed a scab which overlay a large volume of granulation tissue derived from two sources, one lateral, and the other deep and centrally located. In contrast, SAM-covered wounds contained only a small amount of granulation tissue which was derived solely from lateral sources. Granulation tissue was replaced by fibrous connective tissue over time, and this was always less in SAM-covered wounds. Deposition of large amounts of connective tissue in air-exposed wounds was associated with significant polymorphonuclear and mononuclear cell infiltrates, while the lack of granulation tissue formation in SAM-covered sites was associated with reduced inflammation. Dressing-induced inhibition of connective tissue could be partially reversed by treatment with transforming growth factor-beta form 1 or 2. Deposition of granulation tissue in large lenticular wounds in guinea pig skin, but not in 6-mm punch wounds, was also inhibited when the wounds were covered with SAM, and the morphology of air-exposed and SAM-covered wounds was similar to that in mice. SAM-covered wounds in mice and guinea pigs may be useful as models of chronic non-healing wounds.
Ksander
G A
GA
Celtrix Laboratories, Collagen Corp., Palo Alto, CA 94303.
Pratt
B M
BM
Desilets-Avis
P
P
Gerhardt
C O
CO
McPherson
J M
JM
D000818:Animals; D001458:Bandages; D002460:Cell Line; D003238:Connective Tissue / Q000187:drug effects / Q000473:pathology* / Q000503:physiopathology; D005260:Female; D006168:Guinea Pigs; D008297:Male; D051379:Mice; D009779:Occlusive Dressings; D012867:Skin / Q000187:drug effects / Q000473:pathology*; D015291:Transforming Growth Factors / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects; D014947:Wounds and Injuries / Q000473:pathology* / Q000503:physiopathology
D015291:Transforming Growth Factors