title: [35S]Lanthionine ketimine binding to bovine brain membranes.
abstract: 2H-1,4-Thiazine-5,6-dihydro-3,5-dicarboxylic acid (trivial name: lanthionine ketimine) is a cyclic sulfur-containing imino acid detected in bovine brain extracts. This compound has been synthesized in a heavily labeled form starting from L-[35S]cysteine and purified by high performance liquid chromatography. We demonstrate the existence of a saturable and reversible binding of [35S]lanthionine ketimine to bovine brain membranes. A single population of binding sites with a concentration of 260 +/- 12 fmol/mg protein and a dissociation constant of 58 +/- 14 nM is present. Specific binding is competitively inhibited by other structurally similar imino acids, namely S-aminoethyl-L-cysteine ketimine and cystathionine ketimine. These results suggest a possible functional role for these ketimines in nervous system.
authors.affiliation: Dipartimento di Scienze Biochimiche, Università di Roma, La Sapienza, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Fontana
authors.forename: G
authors.initials: G
authors.lastname: Ricci
authors.forename: S P
authors.initials: SP
authors.lastname: Solinas
authors.forename: A
authors.initials: A
authors.lastname: Antonucci
authors.forename: I
authors.initials: I
authors.lastname: Serao
authors.forename: S
authors.initials: S
authors.lastname: Duprè
authors.forename: D
authors.initials: D
authors.lastname: Cavallini
mesh_terms: D000603:Amino Acids, Sulfur / Q000138:chemical synthesis / Q000378:metabolism*; D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D003540:Cystathionine / Q000031:analogs & derivatives / Q000378:metabolism; D066298:In Vitro Techniques
chemical_list: D000603:Amino Acids, Sulfur; D003540:Cystathionine; C035497:thialysine ketimine; C035499:lanthionine ketimine; C038681:cystathionine ketimine