[The thrombocyte function of bronchial asthma patients].
The functional characteristics of platelets were estimated in patients with different asthma forms (atopic, aspirin-sensitive and mixed ones). It was shown that platelets of asthmatics with the atopic and aspirin-sensitive disease forms are more sensitive to the stimulating action of platelet activating factor (PAF) in aggregation response and intracellular Ca2+ influx induction than platelets of patients suffering mostly from the recurrent bronchopulmonary infections with atopic component (so-called "mixed" asthma) and more activated than platelets of healthy donors. Intracellular Ca2+ influx was measured using the fluorescent probe Fura-2 in platelet suspension. Platelet aggregation was measured on aggregometer by two methods (Born's method and correlational photometric method) in platelet-rich plasma. PAF was used as inductor of platelet aggregation and fluorescence response. The antiallergic drugs ketotifen and sodium cromoglycate (intal) caused a 30-40% inhibition of PAF-induced aggregation of asthmatics' and healthy donors' platelets. It may be concluded that platelets of atopic and aspirin asthmatics are more active, their "hyper-reaction" to PAF is expressed by higher aggregability and the lower PAF concentration threshold.
Emirova
A S
AS
Tatarskiĭ
A R
AR
Chuchalin
A G
AG
D001249:Asthma / Q000097:blood*; D001572:Benzofurans; D001792:Blood Platelets / Q000502:physiology*; D002118:Calcium / Q000097:blood; D005456:Fluorescent Dyes; D016257:Fura-2; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors
D001572:Benzofurans; D005456:Fluorescent Dyes; D010975:Platelet Aggregation Inhibitors; D007073:Immunoglobulin E; D002118:Calcium; D016257:Fura-2
Issledovanie funktsional'nogo sostoianiia trombotsitov u bol'nykh bronkhial'noĭ astmoĭ.