[35S]Lanthionine ketimine binding to bovine brain membranes.
2H-1,4-Thiazine-5,6-dihydro-3,5-dicarboxylic acid (trivial name: lanthionine ketimine) is a cyclic sulfur-containing imino acid detected in bovine brain extracts. This compound has been synthesized in a heavily labeled form starting from L-[35S]cysteine and purified by high performance liquid chromatography. We demonstrate the existence of a saturable and reversible binding of [35S]lanthionine ketimine to bovine brain membranes. A single population of binding sites with a concentration of 260 +/- 12 fmol/mg protein and a dissociation constant of 58 +/- 14 nM is present. Specific binding is competitively inhibited by other structurally similar imino acids, namely S-aminoethyl-L-cysteine ketimine and cystathionine ketimine. These results suggest a possible functional role for these ketimines in nervous system.
Fontana
M
M
Dipartimento di Scienze Biochimiche, Università di Roma, La Sapienza, Italy.
Ricci
G
G
Solinas
S P
SP
Antonucci
A
A
Serao
I
I
Duprè
S
S
Cavallini
D
D
D000603:Amino Acids, Sulfur / Q000138:chemical synthesis / Q000378:metabolism*; D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D003540:Cystathionine / Q000031:analogs & derivatives / Q000378:metabolism; D066298:In Vitro Techniques
D000603:Amino Acids, Sulfur; D003540:Cystathionine; C035497:thialysine ketimine; C035499:lanthionine ketimine; C038681:cystathionine ketimine