The protective effects of cell-free fluid exudate on cartilage degradation in vitro.
In a short-term culture system, polymorphonuclear neutrophils (PMNs) were shown to cause significant loss of glycosaminoglycan (GAG) from cartilage. In addition, a lysate of these cells, and in particular cells stimulated with a phorbol ester, greatly enhanced this loss of GAG. This loss could be partially or completely inhibited by the addition of cell-free fluid exudate to the system. In other long-term culture experiments, IL-1a was shown to enhance fibroblast-induced GAG loss from cartilage. In this system too the breakdown was inhibited by cell-free fluid exudate. Initial characterization of the protective agent or agents shows them to be heat-stable at 56 degrees C and to be non-dialysable. It is proposed that in the chronic inflammatory joint disease, rheumatoid arthritis, the fluid phase of an inflammatory response may have a protective effect against the underlying pathological processes.
Moore
A R
AR
Department of Experimental Pathology, St. Bartholomew's Hospital Medical College, London, England.
Desa
F M
FM
Hanahoe
T H
TH
Colville-Nash
P R
PR
Chander
C L
CL
Howat
D W
DW
Willoughby
D A
DA
D000818:Animals; D001172:Arthritis, Rheumatoid / Q000378:metabolism; D002356:Cartilage / Q000378:metabolism* / Q000473:pathology; D002474:Cell-Free System / Q000378:metabolism; D005122:Exudates and Transudates / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism*; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000494:pharmacology; D008297:Male; D009504:Neutrophils / Q000378:metabolism*; D051381:Rats
D006025:Glycosaminoglycans; D007375:Interleukin-1