title: Soluble and membrane-bound forms of cytochrome b5 are the products of a single gene in chicken.
abstract: In order to determine the relationship of the soluble cytochrome b5 found in erythrocytes to the membrane-bound form found in other tissues, a cDNA clone encoding cytochrome b5 in chicken erythrocytes was isolated by using mixed oligonucleotides based on a partial amino acid sequence of the protein. Complete nucleotide sequence identity between the erythrocyte cDNA and the sequence of a cDNA clone of the liver protein suggests that they are transcribed from the same gene. The isolation and structural analysis of genomic clones was also consistent with the presence of only one cytochrome b5 gene in chicken. These results suggest that the formation of soluble erythrocyte cytochrome b5 occurs by proteolytic processing of the membrane-bound form. Thus, previous reports indicating that the carboxyl terminal amino acid residue of the erythrocyte form differs from the corresponding residue of the membrane-bound form may suggest the existence of a novel post-translational modification.
authors.affiliation: DOE Plant Research Laboratory, Michigan State University, East Lansing 48824.
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.forename: C
authors.initials: C
authors.lastname: Somerville
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002462:Cell Membrane / Q000201:enzymology; D002645:Chickens; D003001:Cloning, Molecular; D015786:Cytochromes b5 / Q000032:analysis / Q000235:genetics*; D004274:DNA, Recombinant; D004912:Erythrocytes / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D012995:Solubility
chemical_list: D004274:DNA, Recombinant; D015786:Cytochromes b5