title: Ratio of fetal to maternal DNA is less than 1 in 5000 at different gestational ages in maternal blood.
abstract: Using Southern hybridization with the DNA probe pY3.4, we were not able to detect fetal DNA in blood of 36 pregnant women carrying male fetuses. Gestational ages ranged from 8-40 weeks of pregnancy. Using the same DNA probe, we were able to detect the male-specific signal in experimental dilution series down to 1/5000 on autoradiograms. We conclude that the ratio of fetal DNA in maternal circulation, in contrast to previous estimations, must be lower than 1/5000.
authors.affiliation: Frauenklinik der Westfälischen Wilhelms-Universität Münster, FRG.
authors.forename: D
authors.initials: D
authors.lastname: Gänshirt-Ahlert
authors.forename: M
authors.initials: M
authors.lastname: Pohlschmidt
authors.forename: A
authors.initials: A
authors.lastname: Gal
authors.forename: P
authors.initials: P
authors.lastname: Miny
authors.forename: J
authors.initials: J
authors.lastname: Horst
authors.forename: W
authors.initials: W
authors.lastname: Holzgreve
mesh_terms: D001345:Autoradiography; D015139:Blotting, Southern; D004247:DNA / Q000097:blood*; D015342:DNA Probes; D005314:Embryonic and Fetal Development; D005260:Female; D005865:Gestational Age; D006801:Humans; D006824:Hybridization, Genetic; D008297:Male; D008967:Molecular Biology; D016133:Polymerase Chain Reaction; D011247:Pregnancy / Q000097:blood* / Q000235:genetics; D011296:Prenatal Diagnosis / Q000379:methods; D012737:Sex Factors
chemical_list: D015342:DNA Probes; D004247:DNA