Molecular cloning and characterization of human pyruvate dehydrogenase beta subunit gene.
A genomic clone encompassing the entire gene for the human pyruvate dehydrogenase beta subunit (PDH beta) has been isolated by screening a leukocyte genomic library with a nick-translated human foreskin fibroblast PDH beta cDNA probe. The 18-kilobase clone was characterized by restriction enzyme analysis, extensive DNA sequencing, and primer-extension analysis. The PDH beta structural gene is composed of 10 exons and 9 introns. All intron-exon splice junctions follow the GT/AG rule. The Alu family was found in introns 2 and 8. The 5' flanking region of the PDH beta gene contains a "CAAT" consensus promoter sequence but no "TATA" sequence. Primer-extension analysis indicated that the PDH beta gene transcription start site is an adenine residue located 132 bases upstream from the initiation codon in exon 1.
Koike
K
K
Department of Pathological Biochemistry, Nagasaki University School of Medicine, Japan.
Urata
Y
Y
Koike
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D005091:Exons; D005822:Genetic Vectors; D015698:Genomic Library; D006801:Humans; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D011768:Pyruvate Dehydrogenase Complex / Q000235:genetics*; D015183:Restriction Mapping
D046911:Macromolecular Substances; D015345:Oligonucleotide Probes; D011768:Pyruvate Dehydrogenase Complex