Biodistribution of p-iodobenzoyl (PIP) labeled antibodies in a murine lymphoma model.
A monoclonal antibody against the murine T-cell antigen Thy 1.1 was radioiodinated using N-succinimidyl p-iodobenzoate (PIP) in an attempt to decrease deiodination of the labeled antibody. The biodistribution of the PIP labeled antibody was compared to Iodogen labeled antibody in Thy 1.1+ lymphoma bearing AKR/Cum mice, where the antibody was tumor specific, and AKR/J mice where the antibody reacted with both tumor and normal T-cells. PIP labeling resulted in decreased iodine concentrations in stomach and salivary gland as compared to Iodogen labeling. There was little difference in radioiodine concentrations between the two preparations in tumor, lymphoid tissues or other organs. These results suggest deiodination of intact antibody plays little role in the clearance of radioiodinated anti-Thy 1.1 antibody from tissues.
Badger
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CC
Division of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
Wilbur
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DS
Hadley
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Fritzberg
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AR
Bernstein
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ID
D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics*; D007457:Iodine Radioisotopes; D007463:Iodobenzoates; D007553:Isotope Labeling / Q000379:methods; D008223:Lymphoma / Q000378:metabolism*; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D009368:Neoplasm Transplantation; D014018:Tissue Distribution; D014508:Urea / Q000031:analogs & derivatives
D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes; D007463:Iodobenzoates; C012385:1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenylglycoluril; D014508:Urea; C062337:N-succinimidyl 3-iodobenzoate