title: Argyrophilic nucleolar organizer region proteins (Ag-NORs) in human brain tumors: relations with grade of malignancy and proliferation indices.
abstract: Proliferation indices and mean number of silver-stained nucleolar organizer region-associated proteins (Ag-NORs) are compared in 65 brain tumors, including 34 gliomas, 8 meningiomas, 17 metastatic tumors, and 6 other tumors. Immunocytochemical investigations include labeling with the monoclonal antibody Ki-67 which identifies the whole growth fraction, and with a monoclonal antibody against bromodeoxyuridine (BrdUrd) which detects cells in the S phase of the cell cycle after in vitro incubation with BrdUrd. When all types of tumors are collectively considered, mean numbers of Ag-NORs did not correlate with Ki-67 and Brd-Urd labeling indices (LIs) and mitotic index. Among tumor subtypes, only meningiomas showed significant correlations between Ag-NOR counts, LIs, and malignancy. Mean number of Ag-NORs did not correlate with proliferation indices and tumor grade in low-grade and high-grade gliomas. However, recurrent high-grade gliomas showed a tendency to higher Ag-NOR counts than primary tumors. This study indicates that counting of Ag-NORs in paraffin sections is of limited value in tumor neuropathology. Correlations found in meningeal tumors should be substantiated in larger series.
authors.affiliation: Pathologisches Institut, Universität Innsbruck, Austria.
authors.forename: H
authors.initials: H
authors.lastname: Maier
authors.forename: T
authors.initials: T
authors.lastname: Morimura
authors.forename: D
authors.initials: D
authors.lastname: Ofner
authors.forename: C
authors.initials: C
authors.lastname: Hallbrucker
authors.forename: K
authors.initials: K
authors.lastname: Kitz
authors.forename: H
authors.initials: H
authors.lastname: Budka
mesh_terms: D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000473:pathology* / Q000556:secondary; D002453:Cell Cycle; D002455:Cell Division; D002466:Cell Nucleolus / Q000648:ultrastructure*; D002648:Child; D005260:Female; D005910:Glioma / Q000473:pathology; D006801:Humans; D008297:Male; D008577:Meningeal Neoplasms / Q000473:pathology; D008579:Meningioma / Q000473:pathology; D008875:Middle Aged; D008940:Mitotic Index; D009687:Nuclear Proteins / Q000032:analysis*
chemical_list: D009687:Nuclear Proteins