title: An easy method for impaling cells using the micromanipulation technique.
abstract: In the method described, the head of a fixed-stage microscope and the attached micromanipulator are simultaneously displaced during focussing movements of the microscope. This design allows axial displacements of the microscope towards the cell to be injected. This arrangement makes the filling of neurons in brain slices by intracellular iontophoresis injection of Lucifer yellow considerably easier.
authors.affiliation: Department of Neurosciences, Universidad del País Vasco, Leioa, Spain.
authors.forename: A L
authors.initials: AL
authors.lastname: Perez-Samartin
authors.forename: L
authors.initials: L
authors.lastname: Martinez-Millan
authors.forename: F
authors.initials: F
authors.lastname: Doñate-Oliver
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology*; D002415:Cats; D005456:Fluorescent Dyes; D007478:Iontophoresis / Q000295:instrumentation* / Q000379:methods; D007546:Isoquinolines; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D007546:Isoquinolines; C017475:lucifer yellow