A novel TIMP-insensitive type IV collagen-degrading metalloproteinase from murine metastatic sarcoma cells.
A novel type IV collagen-degrading metalloproteinase was purified from the conditioned media of a murine metastatic sarcoma cell line. The molecular weight of the purified enzyme was determined to be 100 kDa by SDS-PAGE, while 700 kDa by gel filtration suggesting that the enzyme has a multimer structure. This enzyme degrades type IV collagen, but neither type I collagen nor casein. The failure of trypsin treatment to enhance the enzyme activity suggested that the purified enzyme did not require activation. Although the enzyme seems to be classified as a matrix metalloproteinase, it was inhibited by neither tissue inhibitor of metalloproteinases (TIMP) nor TIMP-2 and thus represents a novel type IV collagen-degrading metalloproteinase.
Kato
Y
Y
Bio-Science Laboratory, Asahi Chemical Industry Co. Ltd., Shizuoka, Japan.
Ogawa
K
K
Yamamoto
S
S
Abe
S
S
Kishi
J
J
Hayakawa
T
T
D000818:Animals; D003094:Collagen / Q000378:metabolism*; D006023:Glycoproteins / Q000494:pharmacology; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000494:pharmacology; D012513:Sarcoma, Experimental / Q000201:enzymology*; D019716:Tissue Inhibitor of Metalloproteinase-2; D019714:Tissue Inhibitor of Metalloproteinases
D006023:Glycoproteins; D009363:Neoplasm Proteins; D019714:Tissue Inhibitor of Metalloproteinases; D019716:Tissue Inhibitor of Metalloproteinase-2; D003094:Collagen; D008666:Metalloendopeptidases