Identification of a membrane glycoprotein overexpressed in murine lymphoma sublines resistant to cis-diamminedichloroplatinum(II).
A series of murine thymic lymphoma cell sublines was selected in vitro for resistance to cis-diamminedichloroplatinum(II) (CDDP). The level of CDDP resistance correlated with reduced drug accumulation in these cells. A rabbit antiserum was raised against the plasma membrane of a CDDP-resistant subline and used in Western blot analyses. Increased expression of a surface antigen of approximately 200 kDa was observed and found to correlate with the degree of resistance. Further biochemical and immunological studies demonstrated that this is a plasma membrane glycoprotein. However, it is different from the multidrug resistance-associated P-glycoprotein with a molecular weight of about 170,000. We have called this unique CDDP resistance-associated membrane protein CPR-200.
Kawai
K
K
Ontario Cancer Institute, Princess Margaret Hospital, Toronto, Canada.
Kamatani
N
N
Georges
E
E
Ling
V
V
D000818:Animals; D002460:Cell Line; D002945:Cisplatin / Q000494:pharmacology*; D004317:Doxorubicin / Q000494:pharmacology; D004351:Drug Resistance; D004591:Electrophoresis, Polyacrylamide Gel; D007118:Immunoassay; D007700:Kinetics; D008223:Lymphoma; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000302:isolation & purification; D051379:Mice; D008970:Molecular Weight; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000378:metabolism; D014747:Vinblastine / Q000494:pharmacology
D008562:Membrane Glycoproteins; D014747:Vinblastine; D004317:Doxorubicin; D002945:Cisplatin