Development of a genetic transformation system for Histoplasma capsulatum: complementation of uracil auxotrophy.
We have developed a simple and efficient transformation system for the dimorphic fungus Histoplasma capsulatum. Mutants of H. capsulatum defective in orotidine-5'-monophosphate pyrophosphorylase were transformed to prototrophy by the cloned URA5 gene of the filamentous fungus Podospora anserina. Abortive and mitotically stable transformants were obtained. The stable transformants had integrated copies of the plasmid, some in tandem head-to-tail orientation. Free plasmid identical to the transforming plasmid was present in some of the transformants. We obtained a transformation efficiency of up to 30 transformants/micrograms DNA for plasmid pPAura5-1 (9.2 kb). pPW2001, a smaller plasmid (4.7 kb) derived from pPAura5-1, transformed H. capsulatum more efficiently (up to 155 transformants/micrograms DNA).
Worsham
P L
PL
Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Mo 63110.
Goldman
W E
WE
D004261:DNA Replication; D004271:DNA, Fungal / Q000235:genetics*; D005816:Genetic Complementation Test; D006658:Histoplasma / Q000201:enzymology / Q000235:genetics*; D008938:Mitosis; D008970:Molecular Weight; D009962:Orotate Phosphoribosyltransferase / Q000378:metabolism; D010957:Plasmids; D014170:Transformation, Genetic; D014498:Uracil
D004271:DNA, Fungal; D014498:Uracil; D009962:Orotate Phosphoribosyltransferase