Purification of pyruvate kinase from human erythrocytes by immunoaffinity chromatography.
An one-step-purification of native pyruvate kinase from human erythrocytes by immunoaffinity chromatography with monoclonal antibodies coupled on CNBr-activated Sepharose 4B is described. The recoveries of PK amounted up to 83%, with a 2400 fold purification of PK-R from human stroma free hemolysate.
Kuckelkorn
U
U
Institute of Biochemistry, Humboldt University, Berlin, GDR.
Jacobasch
G
G
D000911:Antibodies, Monoclonal / Q000276:immunology; D002846:Chromatography, Affinity; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007527:Isoenzymes / Q000276:immunology / Q000302:isolation & purification*; D011770:Pyruvate Kinase / Q000276:immunology / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D011770:Pyruvate Kinase