Measurement of intraocular IgA and IgM synthesis and filtration through the blood-aqueous barrier in cataract patients.
The authors have tested two formulas by means of which the filtrated and the intraocularly (intrathecally) synthesized fraction of particular protein can be distinguished. The laser nephelometric method was applied to determine the serum (IgG, IgA, IgM, C3, albumin), aqueous humor (IgA, IgM, albumin) and CSF (IgG, IgM, C3, albumin) protein values in 42 subjects to test two models in physiological conditions. It was confirmed that the best results were achieved using the formula after Stambuk. Computer simulation performed to compare the formulas after Stambuk and Reiber & Felgenhauer showed a strong correlation (r greater than 0.98) between the calculated filtrated fractions of both models in a wide range of different barrier permeabilities. Laser nephelometry proved to be a useful method to determine low IgA and IgM values in aqueous humor.
Stambuk
N
N
Institute for Medical Research and Occupational Health, Zagreb, Yugoslavia.
Curković
T
T
Trbojević-Cepe
M
M
Ozegović
J
J
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000418:Albumins / Q000096:biosynthesis / Q000134:cerebrospinal fluid; D001082:Aqueous Humor / Q000276:immunology*; D002386:Cataract / Q000276:immunology*; D003176:Complement C3 / Q000096:biosynthesis / Q000134:cerebrospinal fluid; D003198:Computer Simulation; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000096:biosynthesis* / Q000134:cerebrospinal fluid; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000134:cerebrospinal fluid; D007075:Immunoglobulin M / Q000096:biosynthesis* / Q000134:cerebrospinal fluid; D008297:Male; D008433:Mathematics; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry
D000418:Albumins; D003176:Complement C3; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M