Effects of supraphysiologic temperature and broth dilution on serum protein binding.
Pooled human serum, unheated (UU) and heated at 56 degrees C for 30 (HU-30) and 60 (HU-60) min, and these media diluted 1:1 with broth (UD, HD-30, and HD-60) were used to assess the effects of supraphysiologic temperature and broth dilution on the serum protein binding of ceftriaxone. Protein binding was determined by ultrafiltration and subsequent high-performance liquid chromatography analysis. Significant differences in protein binding between UU and HU-60 (P less than 0.05), UD and HD-60 (P less than 0.01), HD-30 and HD-60 (P less than 0.01), and all undiluted and diluted media (P less than 0.01) were found. These alterations in protein binding may influence the in vitro microbiologic testing of highly protein-bound antibiotics.
White
R L
RL
College of Pharmacy, Medical University of South Carolina, Charleston 29425-0810.
Kays
M B
MB
Armstrong
T A
TA
Friedrich
L V
LV
D000328:Adult; D000900:Anti-Bacterial Agents / Q000097:blood*; D001798:Blood Proteins / Q000378:metabolism*; D002443:Ceftriaxone / Q000097:blood; D003470:Culture Media; D006358:Hot Temperature; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D011485:Protein Binding
D000900:Anti-Bacterial Agents; D001798:Blood Proteins; D003470:Culture Media; D002443:Ceftriaxone