Lysosomal depletion in macrophages from spleen and foot lesions of Leishmania-infected hamster.
Analysis of lysosomes through acid phosphatase cytochemistry at the electron microscopy level has been performed in spleen and foot lesions from Leishmania-infected hamsters. The results showed that there is lysosomal depletion in macrophages from Leishmania donovani chagasi-infected hamster spleen and similar findings were obtained from foot lesions of Leishmania mexicana amazonensis-infected hamsters. The distribution of acid phosphatase and thiamine pyrophosphatase was also examined in the Golgi apparatus. It was possible to demonstrate that the activity of ACP is absent in infected macrophages from spleen and foot lesions of Leishmania-infected hamster while the distribution of TPP was very similar in control and infected macrophages from both systems. These results provide evidence that the lysosomal depletion can occur at the ACP synthesis and/or glycosylation level.
Barbiéri
C L
CL
Department of Microbiology, Immunology and Parasitology, Escola Paulista de Medicina, São Paulo, Brasil.
Doine
A I
AI
Freymuller
E
E
D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D006056:Golgi Apparatus / Q000201:enzymology; D006651:Histocytochemistry; D007893:Leishmania donovani / Q000201:enzymology / Q000648:ultrastructure; D007894:Leishmania mexicana / Q000201:enzymology / Q000648:ultrastructure; D007896:Leishmaniasis / Q000201:enzymology*; D007898:Leishmaniasis, Visceral / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology* / Q000648:ultrastructure; D008264:Macrophages / Q000201:enzymology* / Q000648:ultrastructure; D008647:Mesocricetus; D008854:Microscopy, Electron; D013154:Spleen / Q000201:enzymology / Q000469:parasitology / Q000648:ultrastructure; D013834:Thiamine Pyrophosphatase / Q000032:analysis
D000135:Acid Phosphatase; D013834:Thiamine Pyrophosphatase