L428 cells derived from Hodgkin's disease produce E rosette-inhibiting factor.
Diminished rosetting capacity of T cells is a well-known phenomenon in Hodgkin's disease, and inhibitors of E rosette formation have been reported to be present in the plasma of patients with Hodgkin's disease. The cell line L428, representing an in vitro counterpart of Hodgkin and Sternberg-Reed cells, could be shown to release a factor capable of suppressing the binding of sheep red blood cells (SRBC) to normal peripheral-blood T lymphocytes or to a T-cell line (L735). At maximally effective concentrations, RIF (rosette inhibiting factor) inhibited T lymphocyte rosetting by approximately 40% (mean from 184 healthy controls). The diminished E rosetting of T lymphocytes from Hodgkin's patients was not further suppressed by added RIF. This factor inhibited binding of SRBC to their target cells at 37 degrees C but not at 4 degrees C. The factor could be stored lyophilized at -20 degrees C and was stable at 56 degrees C (30 minutes). RIF was inactive below pH 6 and above pH 9 or after trypsin digestion. Purification by affinity, ion exchange, and molecular sieve chromatography showed activity peaks at 12.5 Kd, 25 Kd, 50 Kd, and 100 Kd.
Katay
I
I
Medizinische Klinik I, der Universität zu Köln, West Germany.
Wirnitzer
U
U
Burrichter
H
H
von Kalle
C
C
Schell-Frederick
E
E
Diehl
V
V
Schaadt
M
M
D002460:Cell Line; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004912:Erythrocytes / Q000473:pathology; D006689:Hodgkin Disease / Q000097:blood / Q000473:pathology*; D006801:Humans; D008077:Lipoproteins, LDL / Q000302:isolation & purification / Q000378:metabolism* / Q000502:physiology
D008077:Lipoproteins, LDL; C022060:rosette-inhibiting factor