title: Improved fragile site detection with trimethoprim.
abstract: The effect of trimethoprim, an inhibitor of dihydrofolate reductase, in demonstrating the presence of fragile sites on human chromosomes was investigated. Lymphocyte cultures with 20 mg/l trimethoprim added at the onset of incubation gave consistently higher frequencies of fragile sites than the other culture regimes tested.
authors.affiliation: Department of Pathology, University of Dundee, Ninewells Hospital, UK.
authors.forename: D A
authors.initials: DA
authors.lastname: McLean
authors.forename: M J
authors.initials: MJ
authors.lastname: Faed
mesh_terms: D043283:Chromosome Fragile Sites; D002873:Chromosome Fragility; D005260:Female; D005600:Fragile X Syndrome / Q000175:diagnosis*; D006801:Humans; D008297:Male; D012729:Sex Chromosome Aberrations / Q000175:diagnosis*; D012737:Sex Factors; D014295:Trimethoprim
chemical_list: D014295:Trimethoprim