title: Proline uptake in Streptomyces clavuligerus.
abstract: Streptomyces clavuligerus was able to accumulate proline intracellularly throughout a wide range of external proline concentrations. Kinetic analysis of proline uptake indicated that this phenomenon is mediated by two saturable systems. One of them was a high-affinity system (Km = 11 microM), with low uptake capacity and specific for proline. The second system had lower affinity for proline (Km = 10.2 mM), higher uptake capacity, and was inhibited by several amino acids. Proline was not required as an inducer of the systems, which were neither repressed nor inhibited by ammonium.
authors.affiliation: Laboratorio de Microbiología, Departamento de Biología Funcional, Facultad de Medicina, Universidad de Oviedo, Spain.
authors.forename: V
authors.initials: V
authors.lastname: Bascarán
authors.forename: C
authors.initials: C
authors.lastname: Hardisson
authors.forename: A F
authors.initials: AF
authors.lastname: Braña
mesh_terms: D007700:Kinetics; D009584:Nitrogen / Q000494:pharmacology; D011392:Proline / Q000378:metabolism*; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D011392:Proline; D009584:Nitrogen