[The isolation of the ribosomal fraction of Clostridium perfringens type A and an evaluation of its protective activity].
A method for isolation of the ribosomal fraction (RF) from the cytoplasm of type-A C. perfringens strain BP6K was developed and its chemical and antigenic properties characterized. RF has been found to possess protective properties: two subcutaneous immunizations of mice with RF preparations adsorbed on Al(OH)3 in doses of 250 and 500 micrograms (dry weight) has ensured, on the average, the protection of 41.9% of the immunized animals from 1 DCL of type-A C. perfringens strain BP6K culture.
Bakulin
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D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D003016:Clostridium perfringens / Q000276:immunology / Q000302:isolation & purification*; D004306:Dose-Response Relationship, Immunologic; D004353:Drug Evaluation, Preclinical; D005738:Gas Gangrene / Q000276:immunology / Q000628:therapy; D007114:Immunization; D051379:Mice; D012269:Ribosomal Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000627:therapeutic use
D012269:Ribosomal Proteins
Poluchenie ribosomal'noĭ fraktsii Clostridium perfringens tipa A i otsenka ee protektivnoĭ aktivnosti.