Use of a synthetic oligonucleotide probe to detect strains of non-serovar O1 Vibrio cholerae carrying the gene for heat-stable enterotoxin (NAG-ST).
A synthetic oligonucleotide probe was developed to identify the gene for the heat-stable enterotoxin (NAG-ST) of non-serovar O1 Vibrio cholerae. Of 103 non-O1 V. cholerae isolates from Thailand, 31 isolates from Mexico, and 47 isolates from patients in the United States, only 7 (all from Thailand) hybridized with the probe. Probe-positive strains produced significantly higher fluid accumulations in infant mice than probe-negative strains.
Hoge
C W
CW
Department of Medicine, University of Maryland School of Medicine, Baltimore 21201.
Sethabutr
O
O
Bodhidatta
L
L
Echeverria
P
P
Robertson
D C
DC
Morris
J G
JG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D004768:Enterotoxins / Q000235:genetics*; D005798:Genes, Bacterial; D006801:Humans; D007223:Infant; D051379:Mice; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D014734:Vibrio cholerae / Q000235:genetics* / Q000302:isolation & purification
D004768:Enterotoxins; D015345:Oligonucleotide Probes; C037420:stN protein, Vibrio cholerae