Structural analysis of xyloglucan oligosaccharides by 1H-n.m.r. spectroscopy and fast-atom-bombardment mass spectrometry.
A method to determine rapidly the identities and proportions of the oligosaccharide repeating-units in plant cell-wall xyloglucans by 1D 1H-n.m.r. spectroscopy was developed. Six of the most commonly found xyloglucan oligosaccharide subunits (including three subunits that had not been fully characterized previously) were prepared by endo-(1----4)-beta-D-glucanase digestion of xyloglucans from various plant species. The oligosaccharides were reduced to the corresponding oligoglycosyl-alditols, purified, and characterized by glycosyl composition and linkage analysis, 1H-n.m.r. spectroscopy, and f.a.b.-mass spectrometry. Correlations between the 1H-n.m.r. spectra and the structures of the oligoglycosyl-alditols can be used to identify oligoglycosyl-alditols derived from xyloglucans of unknown structure. The identities and relative amounts of the oligosaccharide subunits of xyloglucans isolated from tamarind seed and rapeseed hulls were determined on this basis.
York
W S
WS
Complex Carbohydrate Research Center, University of Georgia, Athens 30602.
van Halbeek
H
H
Darvill
A G
AG
Albersheim
P
P
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D005936:Glucans; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000145:classification; D010944:Plants / Q000032:analysis; D011134:Polysaccharides / Q000145:classification; D009626:Terminology as Topic; D014990:Xylans
D005936:Glucans; D009844:Oligosaccharides; D011134:Polysaccharides; D014990:Xylans; C029353:xyloglucan; D006859:Hydrogen