Production, purification and some properties of cholesterol oxidase from a Streptomyces Sp.
A Streptomyces Sp strain producing appreciable levels of cholesterol oxidase was selected. In the culture medium, cholesterol oxidase activity can reach 13,000 UI/l. Cholesterol oxidase was purified by means of centrifugation, tangential ultrafiltration, ion-exchange chromatography and gel filtration with a yield of 42% to a specific activity of 54 UI/mg. Its molecular weight determined by SDS-PAGE was 55,000. Its optimum activity pH is about 7.
Lartillot
S
S
Department of Applied Biology, Institut Universitaire de Technologie, Dijon, France.
Kedziora
P
P
D015096:3-Hydroxysteroid Dehydrogenases / Q000302:isolation & purification*; D002498:Centrifugation; D002789:Cholesterol Oxidase / Q000302:isolation & purification* / Q000378:metabolism; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D013302:Streptomyces / Q000201:enzymology*; D014461:Ultracentrifugation
D015096:3-Hydroxysteroid Dehydrogenases; D002789:Cholesterol Oxidase