Effects of FeSO4 on B16 melanoma cells differentiation and proliferation.
The effects exerted by FeSO4, in the presence or absence of vitamin C, on melanogenesis and proliferation in mouse B16 melanoma cells in culture were analysed either in serum-free (MEM-N2) or in serum-supplemented media. These cellular parameters can be either stimulated or on the contrary inhibited, depending on the metal concentration, the presence or the absence of vitamin C and serum, and on the type of culture (subconfluent or clonal). Vitamin C toxicity for B16 cells was decreased in the presence of FeSO4.
De Pauw-Gillet
M C
MC
Department of Histology and Cytology, Faculty of Medicine, Université de Liège, Belgium.
Siwek
B
B
Pozzi
G
G
Sabbioni
E
E
Bassleer
R J
RJ
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D011232:Chemical Precipitation; D003470:Culture Media; D005296:Ferrous Compounds / Q000378:metabolism / Q000494:pharmacology*; D008543:Melanins / Q000032:analysis; D008546:Melanoma, Experimental / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL
D003470:Culture Media; D005296:Ferrous Compounds; D008543:Melanins; C020748:ferrous sulfate; D001205:Ascorbic Acid