Effects of pH on antipyrine transfer across the human placenta ex vivo.
The influence of pH variations on transplacental transfer of antipyrine was studied using a human placental cotyledon perfused ex vivo. The antipyrine transfer rate is positively correlated with the pH in the fetal circulation and negatively correlated with the pH in the maternal circulation. Thus, the transfer rate is negatively correlated with the difference between pH values in maternal and fetal circulations. The antipyrine transfer rate is also positively correlated with the flows in maternal and fetal circulations. The above parameters allowed to explain 50% of the variance on the transfer rates obtained in various experimental conditions. In a final series of experiments where these parameters for each placenta were fixed at identical values, a good reproducibility in the results was obtained, the variation coefficient being 17%. Thus, establishing the effect of variations in pH allowed a good standardization of the perfused cotyledon model. This effect cannot be explained by modifications in the ionized fraction of the antipyrine molecular and is probably due to physiological mechanisms.
Giroux
M
M
Groupe de Recherche en Physiologie Obstétricale et Pharmacologie Périnatale, Inserm U-168, CHU la Grave, Toulouse, France.
Campistron
G
G
Faure
F
F
Dumas
J C
JC
Coulais
Y
Y
Desprats
R
R
Grandjean
H
H
Houin
G
G
Pontonnier
G
G
D000328:Adult; D000983:Antipyrine / Q000493:pharmacokinetics*; D005260:Female; D005333:Fetus / Q000098:blood supply; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008954:Models, Biological; D010477:Perfusion; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D012039:Regional Blood Flow / Q000187:drug effects
D000983:Antipyrine