Transcellular transport of angiotensin II through a cultured arterial endothelial monolayer.
We have studied the mechanisms of angiotensin II (A-II) transport through a cultured arterial endothelial cell monolayer. The transport of 125I-labeled A-II was inhibited by excess unlabeled A-II (50 microM) and [Sar1, Ile8]-A-II (50 microM), but was not inhibited by bradykinin (50 microM). The transport process was shown to be temperature dependent and was inhibited by 10 mM NaN3 plus 50 mM 2-deoxyglucose. Monensin (50 microM), an inhibitor of endocytotic trafficking, reduced the rate of transport of 125I-A-II. It is also shown that the specific pathway for A-II transport was unidirectional from the apical to the basolateral surface of the endothelial cell monolayer.
Mineo
C
C
Department of Microbiology and Molecular Pathology, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Yagyu
Y
Y
Imanaka
T
T
Takano
T
T
D000255:Adenosine Triphosphate / Q000502:physiology; D000643:Ammonium Chloride / Q000494:pharmacology; D000804:Angiotensin II / Q000378:metabolism*; D000818:Animals; D001152:Arsenicals / Q000494:pharmacology; D001667:Binding, Competitive; D001692:Biological Transport / Q000187:drug effects; D002850:Chromatography, Gel; D004730:Endothelium, Vascular / Q000378:metabolism*; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D008985:Monensin / Q000494:pharmacology; D013696:Temperature
D001152:Arsenicals; D007457:Iodine Radioisotopes; D000643:Ammonium Chloride; C029341:oxophenylarsine; D000804:Angiotensin II; D000255:Adenosine Triphosphate; D008985:Monensin