Effects of some autacoids on breathing and perfusion flow in the isolated perfused rat lung.
The effects of three autacoids [5-hydroxytryptamine (5-HT), bradykinin and acetylcholine (Ach)) on breathing and pulmonary circulation were studied in the isolated perfused and ventilated rat lung after intravascular injection or intrabronchial instillation. 5-HT, bradykinin and Ach all caused a dose related reduction in breathing, after injection into the pulmonary artery, with Ach being the most potent. With regard to effects on the pulmonary circulation, a dose-related reduction in perfusate flow was noted with bradykinin and 5-HT with bradykinin being about 10 times more potent than 5-HT. Ach did not reduce or increase perfusate flow. After intrabronchial instillation only 5-HT caused reduced perfusate flow. Effects on breathing were difficult to evaluate with this route of administration. The isolated perfused and ventilated lung seems to be a usable model to simultaneously study effects on respiration and pulmonary circulation.
Ryrfeldt
A
A
Department of Toxicology, Karolinska Institutet, Stockholm, Sweden.
Olafsdottir
K
K
Berggren
M
M
Moldéus
P
P
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D001920:Bradykinin / Q000494:pharmacology*; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D008168:Lung / Q000502:physiology*; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D012119:Respiration / Q000187:drug effects*; D012701:Serotonin / Q000494:pharmacology*
D012701:Serotonin; D000109:Acetylcholine; D001920:Bradykinin; D009638:Norepinephrine