[Effect of macrophage-derived growth factor on the cell cycle kinetics of cultured arterial smooth muscle cells].
Effects of macrophage-derived growth factor on the progression of the cell cycle of cultured aortic smooth muscle cells were investigated by using rabbit peritoneal macrophage conditioned-medium (M phi-CM) as the source of the growth factors. DNA content of individual SMC in the cell population which had been exposed either to platelet-poor plasma serum (PPPS) or to M phi-CM was also measured by flow cytometry and microspectrophotometry, as well as by phase contrast microscopy. The results showed that the cells quiescent in PPPS medium had a high population not only in G0/G1 but also in G2 phase. This indicated that the cell cycle process was blocked both in G0/G1 and G2 phase. The cell cycle distribution of SMCs exposed to M phi-CM for 48 hours was very similar to that cultured in 10% fetal calf serum medium. It is suggested that M phi-CM can promote the cell growth-arrested in ppps medium passing through G0/G1 into S phase as well as through G2 into M phase.
Qiu
H M
HM
Department of Pathology, Tongji Medical University.
D000818:Animals; D001011:Aorta; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D005434:Flow Cytometry; D006133:Growth Substances / Q000494:pharmacology*; D008264:Macrophages; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000378:metabolism; D011817:Rabbits
D006133:Growth Substances; D004247:DNA