title: Solid-surface room-temperature phosphorescence detection for high-performance liquid chromatography.
abstract: In the present study, we have optimized the use of a two nebulizer system for solid-surface room-temperature phosphorescence (SSRTP) as a selective, permanent record detector for high-performance liquid chromatography (HPLC). The chromatographic parameters and the analytical figures of merit of five well known phosphorescent compounds were compared to those obtained by ultraviolet detection. Calibration curves with satisfactory linear dynamic ranges and limits of detection in the nanogram and subnanogram level showed the feasibility of the SSRTP detector for HPLC. In addition, overlapped compounds were individually identified demonstrating that the selectivity of the proposed detector can be a useful feature in case of incomplete chromatographic separations of complex mixtures.
authors.affiliation: Department of Chemistry, University of Florida, Gainesville 32611.
authors.forename: A D
authors.initials: AD
authors.lastname: Campiglia
authors.forename: A
authors.initials: A
authors.lastname: Berthod
authors.forename: J D
authors.initials: JD
authors.lastname: Winefordner
mesh_terms: D001580:Benzopyrenes; D002227:Carbazoles; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005449:Fluorenes; D008163:Luminescent Measurements; D010616:Phenanthrenes; D011721:Pyrenes; D013696:Temperature; D014466:Ultraviolet Rays
chemical_list: D001580:Benzopyrenes; D002227:Carbazoles; D005449:Fluorenes; D010616:Phenanthrenes; D011721:Pyrenes; C007738:fluoranthene; C026487:benzo(e)pyrene