Determination of belfosdil, a new calcium channel blocker, in human plasma by capillary gas chromatography with nitrogen-phosphorus detection.
Belfosdil and the internal standard were extracted from human plasma by a double liquid-liquid extraction. After a concentration step, gas chromatographic analysis of the sample was performed using a capillary fused-silica column and a nitrogen-phosphorus detector. The limit of detection of belfosdil was 0.025 ng/ml and the standard curve was linear over the range 0.05-100 ng/ml. The intra-assay and inter-assay precisions were within 7% (relative standard deviation) and the intra-assay and inter-assay accuracy values deviated by less than 5%. The extractability of belfosdil was 79%. The assay method was successfully used for the analysis of plasma samples from clinical studies with dose ranges of 5-100 mg of belfosdil.
Mulvana
D E
DE
Department of Metabolism and Pharmacokinetics, Bristol-Myers Company, Syracuse, NY 13221.
Kaul
S
S
Dandekar
K A
KA
Pittman
K A
KA
D002121:Calcium Channel Blockers / Q000097:blood* / Q000652:urine; D002849:Chromatography, Gas / Q000379:methods*; D004164:Diphosphonates / Q000097:blood* / Q000652:urine; D004355:Drug Stability; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D009584:Nitrogen; D010758:Phosphorus; D011786:Quality Control; D012997:Solvents
D002121:Calcium Channel Blockers; D004164:Diphosphonates; D012997:Solvents; C061154:belfosdil; D010758:Phosphorus; D009584:Nitrogen