[Lysosomotropic agents inhibit the penetration of arenaviruses into a culture of BHK-21 and Vero cells].
Lysosomotropic agents (NH4Cl, amantadine, chloroquine, monensin) which prevent acidification of intracellular vacuoles, when introduced into the culture medium before or during inoculation of cells (BHK-21, Vero) with arenaviruses inhibit reproduction of these viruses completely or significantly. Mozambique virus proved to be 10 times more sensitive to the effect of lysosomotropic agents than Pichinde and Lassa viruses. Thus, arenaviruses have a pH-dependent stage at the beginning of the reproduction cycle which is indirectly indicative of their penetration into cells by receptor endocytosis.
Glushakova
S E
SE
Iakuba
A I
AI
Vasiuchkov
A D
AD
Mar'iankova
R F
RF
Kukareko
T M
TM
Stel'makh
T A
TA
Kurash
T P
TP
Lukashevich
I S
IS
D000327:Adsorption; D000547:Amantadine / Q000494:pharmacology; D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D001116:Arenaviridae / Q000187:drug effects* / Q000472:pathogenicity / Q000502:physiology; D002478:Cells, Cultured / Q000187:drug effects / Q000382:microbiology; D002738:Chloroquine / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007836:Lassa virus / Q000187:drug effects / Q000472:pathogenicity / Q000502:physiology; D008247:Lysosomes / Q000187:drug effects*; D008985:Monensin / Q000494:pharmacology; D013997:Time Factors; D014709:Vero Cells / Q000187:drug effects / Q000382:microbiology; D014779:Virus Replication / Q000187:drug effects
D000643:Ammonium Chloride; D002738:Chloroquine; D008985:Monensin; D000547:Amantadine
Lizosomotropnye agenty ingibiruiut proniknovenie arenavirusov v kul'turu kletok BHK-21 i Vero.