Targeted DNA sequencing: rapid identification of DNA clones by sequencing DNA using mixed oligodeoxynucleotide probes as primers.
A rapid identification method involving targeted DNA sequencing of genomic or cDNA clones using mixed (degenerate) probes as primers is described. The strategy involves the use of the same mixed probes for sequencing the clone of interest as they are used for screening the DNA libraries. Probes containing up to 512 mixes do not interfere in priming and yield completely faithful replication of the template DNA.
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Department of Obstetrics & Gynecology, Albert Einstein College of Medicine, Bronx, NY 10461.
Joshi
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000032:analysis; D004274:DNA, Recombinant / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D004259:DNA-Directed DNA Polymerase; D015698:Genomic Library; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping / Q000379:methods*; D015345:Oligonucleotide Probes
D004274:DNA, Recombinant; D004279:DNA, Viral; D015345:Oligonucleotide Probes; D004247:DNA; C034970:bacteriophage T7 induced DNA polymerase; D004259:DNA-Directed DNA Polymerase