[Cholesterol-hydroxylating cytochrome P-450 from bovine adrenal cortex mitochondria and human placenta: immunochemical properties and structural characteristics].
An immunochemical comparison of components of cholesterol side chain cleavage system from bovine adrenocortical and human placental mitochondria has been carried out. Antibodies against cytochrome P-450scc, adrenodoxin reductase and adrenodoxin from bovine adrenocortical mitochondria were shown to cross-react with corresponding antigens of human placental mitochondria. A highly sensitive immunochemical method for cytochrome P-450scc determination has been developed. Limited proteolysis of cytochrome P-450scc of human placental mitochondria was studied, and the products of trypsinolysis were identified using antibodies against cytochrome P-450scc and fragments of its polypeptide chain: F1, F2 and F3. Immunochemical relatedness of ferredoxins from bovine adrenocortical and human placental mitochondria allowed one to develop a fast and efficient method for cytochrome P-450scc purification from human placental mitochondria by affinity chromatography on adrenodoxin-Sepharose.
Usanov
S A
SA
Chernogolov
A A
AA
Honkakoski
P
P
Lang
M
M
Passanen
M
M
Raunio
H
H
Pelkonen
O
O
D000302:Adrenal Cortex / Q000201:enzymology*; D000818:Animals; D002417:Cattle; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000032:analysis* / Q000302:isolation & purification; D002846:Chromatography, Affinity; D005260:Female; D006801:Humans; D006868:Hydrolysis; D015151:Immunoblotting; D007150:Immunohistochemistry; D008928:Mitochondria / Q000201:enzymology*; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy
D002786:Cholesterol Side-Chain Cleavage Enzyme
Kholesteringidroksiliruiushchiĭ tsitokhrom P-450 mitokhondriĭ kory nadpochechnikov byka i platsenty cheloveka: immunokhimicheskie svoĭstva i strukturnaia kharakteristika.