title: Detection of an antibody response in immunocompetent patients with systemic candidiasis or Candida albicans colonisation.
abstract: Tests to detect circulating antibodies to Candida albicans antigens were performed in sera from 27 immunocompetent patients, 15 of whom had deep-seated candidiasis and 12 of whom were colonised by Candida albicans. For the diagnosis of deep-seated candidiasis in patients with either deep-seated candidiasis or Candida albicans colonisation, counterimmunoelectrophoresis had a sensitivity of 87% and a specificity of 75%. Using immunoblotting it could be shown that antibodies to 35K, 47K, 68K and 88K antigens of Candida albicans occurred more frequently in patients with deep-seated candidiasis than in colonised patients. The presence of dense bands in immunoblots representing antibodies against the 47K and/or 68K antigen served to discriminate significantly between deep-seated and superficial candidiasis (p less than 0.05).
authors.affiliation: Department of Clinical Microbiology, Erasmus University, Rotterdam, The Netherlands.
authors.forename: J C
authors.initials: JC
authors.lastname: Porsius
authors.forename: H J
authors.initials: HJ
authors.lastname: van Vliet
authors.forename: J H
authors.initials: JH
authors.lastname: van Zeijl
authors.forename: W H
authors.initials: WH
authors.lastname: Goessens
authors.forename: M F
authors.initials: MF
authors.lastname: Michel
mesh_terms: D000908:Antibodies, Fungal / Q000097:blood*; D002177:Candidiasis / Q000175:diagnosis / Q000276:immunology*; D015169:Colony Count, Microbial; D003378:Counterimmunoelectrophoresis; D006386:Hemagglutination Tests; D006801:Humans; D015151:Immunoblotting; D007121:Immunocompetence / Q000276:immunology*; D007074:Immunoglobulin G / Q000032:analysis; D011237:Predictive Value of Tests
chemical_list: D000908:Antibodies, Fungal; D007074:Immunoglobulin G