Studies of an oral iron chelator: 1,2-dimethyl-3-hydroxy-pyrid-4-one. I. Iron excretion in rats: development of a new rapid microwave method for iron analysis in faeces.
We have developed a simple, rapid method for analysing faecal iron using a microwave oven for digestion followed by atomic absorption spectrometry. The chelating effect of 1,2-dimethyl-3-hydroxy-pyrid-4-one (DMHP) has been tested in rats with experimental iron overloading and three routes of DHMP administration, oral, subcutaneous, and intraperitoneal, have been compared. Regardless of the route of administration, we have found that DMHP promotes iron excretion via the urine. We have not observed a difference in the amount of iron excreted in the faeces before and after DMHP administration by any route. The subcutaneous route of administration is the most effective in promoting iron excretion, followed by the intraperitoneal route. Although most convenient for clinical use, oral administration promotes the excretion of only a small fraction of that by the subcutaneous route.
Venkataram
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Department of Pharmaceutics, College of Pharmacy, University of Minnesota, Minneapolis 55455.
Rahman
Y E
YE
D000818:Animals; D015913:Chelation Therapy; D000077543:Deferiprone; D004334:Drug Administration Schedule; D005243:Feces / Q000032:analysis*; D007501:Iron / Q000378:metabolism / Q000652:urine; D008297:Male; D008872:Microwaves; D011728:Pyridones / Q000008:administration & dosage / Q000627:therapeutic use*; D051381:Rats; D011919:Rats, Inbred Strains
D011728:Pyridones; D000077543:Deferiprone; D007501:Iron