DNA synthesis by L929 cells following doxorubicin exposure.
Doxorubicin does not kill L929 cells at concentrations that profoundly reduce clonogenic survival. Instead, the cell and nuclear volume progressively increase for at least 1 week following drug exposure leading to the production of characteristic giant cells. The increase in nuclear volume is due to continued DNA synthesis and increase in chromosome number without entry into mitosis. The implications of this finding for in vitro chemosensitivity assays and for the mechanism of doxorubicin cytotoxicity are discussed.
Lanks
K W
KW
Department of Pathology, State University of New York, Health Science Center, Brooklyn 11203-2098.
Lehman
J M
JM
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects*; D004261:DNA Replication / Q000187:drug effects*; D004317:Doxorubicin / Q000494:pharmacology*; D005434:Flow Cytometry; D007621:Karyotyping; D007700:Kinetics; D007739:L Cells / Q000166:cytology / Q000187:drug effects; D051379:Mice
D004317:Doxorubicin; D004247:DNA