Anaphylatoxin and terminal complement complexes in red cell salvage.
Thirteen patients undergoing elective orthopedic surgery were studied regarding anaphylatoxin (C3a and C5a) and terminal complement complex (TCC) formation in association with red cell salvage. The auto-transfusion equipment gave a centrifuged and washed erythrocyte fraction. The concentrations of C5a and TCC were not increased but elevated C3a levels were found in the suspension. After infusion of the erythrocyte fraction to the patient, no signs of systemic complement activation were observed. Thus, plasma levels of C3a, C5a and TCC were within the normal range in all the patients before and after autotransfusion. This study indicates that the complement system is activated in the cellsaver equipment. The washing procedure, however, seems to eliminate most of the anaphylatoxins and terminal complement complexes. No extensive systemic activation of complement seems to occur in association with autotransfusion to patients undergoing elective surgery.
Bengtsson
A
A
Department of Anesthesiology and Intensive Care, Sahlgrens Hospital, Göteborg, Sweden.
Lisander
B
B
D000368:Aged; D000369:Aged, 80 and over; D000706:Anaphylatoxins / Q000032:analysis*; D001804:Blood Transfusion, Autologous / Q000295:instrumentation*; D002469:Cell Separation / Q000295:instrumentation*; D015926:Complement C3a / Q000032:analysis; D015936:Complement C5a / Q000032:analysis; D015938:Complement Membrane Attack Complex / Q000032:analysis*; D017707:Erythrocyte Transfusion; D005260:Female; D006801:Humans; D008875:Middle Aged; D009985:Orthopedics
D000706:Anaphylatoxins; D015938:Complement Membrane Attack Complex; D015926:Complement C3a; D015936:Complement C5a