Isolation of a neuropeptide corresponding to the N-terminal 27 residues of the pituitary adenylate cyclase activating polypeptide with 38 residues (PACAP38).
A novel neuropeptide with 38 residues (PACAP38) was isolated from ovine hypothalamic tissues using the pituitary adenylate cyclase activation in rat pituitary cell cultures as a parameter of the biological activity (Miyata et al, Biochem. Biophys. Res. Commun. 164, 567-574, 1989). From the side fractions obtained during the purification of PACAP38, a shorter form peptide with 27 residues corresponding to the N-terminal 27 amino acids of PACAP38 and amidated C-terminus was isolated and named as PACAP27. Synthetic PACAP27 showed a biological activity of adenylate cyclase stimulation comparable to PACAP38. Moreover PACAP27 which shows a considerable homology with vasoactive intestinal polypeptide (VIP) demonstrated a similar vasodepressor activity as VIP, but the adenylate cyclase stimulating activity was about 1000 times greater than VIP.
Miyata
A
A
U.S.-Japan Biomedical Research Laboratories, Tulane University Hebert Center, Belle Chasse, LA 70037.
Jiang
L
L
Dahl
R D
RD
Kitada
C
C
Kubo
K
K
Fujino
M
M
Minamino
N
N
Arimura
A
A
D000262:Adenylyl Cyclases / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D004789:Enzyme Activation; D007031:Hypothalamus / Q000032:analysis; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000302:isolation & purification* / Q000378:metabolism*; D010455:Peptides / Q000138:chemical synthesis / Q000378:metabolism*; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D010902:Pituitary Gland / Q000201:enzymology*; D012756:Sheep; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism
D009479:Neuropeptides; D010455:Peptides; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D014660:Vasoactive Intestinal Peptide; D000262:Adenylyl Cyclases