Comparison of the promoter regions of H-2Kb and H-2Kbm1 class I MHC genes.
The 2.0 kb-long nucleotide sequences of the promoter regions of two closely related class I genes of the mouse major histocompatibility complex (H-2Kb and H-2Kbm1) have been determined and compared. The promoter sequence of the H-2Kbm1 gene differs from that of the H-2Kb gene by a single deletion of a 'C' at position -456 in the upstream region of H-2Kbm1 gene. The actual existence of this deletion of a single base in genomic DNA has been verified by genomic DNA hybridization, using oligonucleotide probes specific for H-2Kbm1 or H-2Kb respectively. The effect on the enhancer activity of H-2Kbm1 promoter region of the difference at position -456 has been analyzed by the chloramphenicol acetyltransferase (CAT) assay, using appropriate DdeI fragments (-533 to -408 for H-2Kbm1; -534 to -408 for H-2Kb) cloned downstream of pH-2(367)CAT gene construct. The CAT activity determined by the H-2Kbm1 fragment was about 3-fold higher than that of H-2Kb, a result which probably accounts for the higher level of the H-2Kbm1 transcript and antigen in lymph node cells.
Ozawa
K
K
Gene Bank, Life Science Tsukuba Research Center, RIKEN, Institute of Physical and Chemical Research, Ibaraki, Japan.
Saka
F
F
Kitabayashi
I
I
Imai
T
T
Soeda
E
E
Israel
A
A
Gachelin
G
G
Yokoyama
K
K
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005805:Genes, MHC Class I; D006183:H-2 Antigens / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D004247:DNA