Further characterization of urinary aldosterone metabolities in the rabbit by fast atom bombardment mass spectrometry.
After the subcutaneous injection of a large amount of aldosterone into a male rabbit, urine was collected for 24 h. The preliminary separation of urinary aldosterone metabolites was carried out by means of DEAE-Sephadex A-25 column chromatography. Each fraction obtained was further purified by reversed phase high pressure liquid chromatography. Purified materials were then analyzed by fast atom bombardment mass spectrometry and infrared spectroscopy. Thus, tetrahydroaldosterone glucuronide and tetrahydroaldosterone sulfate were detected as urinary aldosterone metabolites. These results confirmed our previously published data, where the nature of conjugating groups was determined indirectly. Furthermore, hydroxyaldosterone was identified as a urinary aldosterone metabolite.
Miyazaki
T
T
First Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Matsuzaki
K
K
D000450:Aldosterone / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D008297:Male; D011817:Rabbits; D013055:Spectrophotometry, Infrared; D014556:Urine / Q000032:analysis*
C065339:3,5-tetrahydroaldosterone sulfate; C013283:tetrahydroaldosterone 3 beta-glucosiduronic acid; D000450:Aldosterone