Three-dimensional computer-aided analysis of the intraganglionic topography of primary muscle afferent neurons in the rat.
A microcomputer system was used to reconstruct, in the L5 dorsal root ganglion (DRG) of the rat, the three-dimensional arrangement of primary neurons which had been labelled by application of horseradish peroxidase (HRP) and fluoro-gold (FG) to various muscle nerves of the leg. Analysis of the data and animation of the reconstructed images with commercially available software were instrumental in identifying the preferential intraganglionic locations of the neurons innervating muscles such as the soleus (SOL), the gastrocnemius lateralis (GL), and medialis (GM), or parts of the GM. These locations appeared to be somewhat related to the position of the muscles in the posterior compartment of the leg. Additionally, the study provided quantitative estimates of muscle afferent neuronal populations, allowed a comparison of the labelling performances of HRP and FG, and finally indicated that few DRG neurons project to two different muscles.
Peyronnard
J M
JM
Centre de Recherche en Sciences Neurologiques, Université de Montréal, Canada.
Messier
J P
JP
Dubreuil
M
M
Charron
L
L
Lebel
F
F
D000818:Animals; D005260:Female; D005456:Fluorescent Dyes; D005727:Ganglia, Spinal / Q000033:anatomy & histology / Q000166:cytology / Q000648:ultrastructure; D006735:Horseradish Peroxidase; D007091:Image Processing, Computer-Assisted; D009132:Muscles / Q000294:innervation*; D009475:Neurons, Afferent / Q000648:ultrastructure*; D051381:Rats; D011919:Rats, Inbred Strains; D013266:Stilbamidines
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D005456:Fluorescent Dyes; D013266:Stilbamidines; D006735:Horseradish Peroxidase