Uptake and deoxyribonuclease 1 susceptibility of foreign deoxyribonucleic acid by chicken sperm cells.
Association of foreign DNA with chicken sperm cells was investigated using a 32P-oligolabelled plasmid preparation. Significant, although low, levels of trichloroacetic acid precipitable radioactivity became rapidly associated with viable sperm cells, in contrast to the situation with formaldehyde-fixed cells. However, analysis by Southern blotting and hybridization of sperm cells incubated with an unlabelled plasmid preparation followed by deoxyribonuclease 1 treatment, demonstrated associated foreign DNA was completely susceptible to nuclease degradation, whereas chromosomal DNA was not affected.
Cook
R F
RF
Department of Dairy Science, Ohio State University, Wooster 44691.
Cook
S J
SJ
Hodgson
C P
CP
D000818:Animals; D002645:Chickens; D004247:DNA / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism*; D008297:Male; D010957:Plasmids; D013094:Spermatozoa / Q000378:metabolism*
D004247:DNA; D003850:Deoxyribonuclease I