title: Pestivirus glycoprotein which induces neutralizing antibodies forms part of a disulfide-linked heterodimer.
abstract: Neutralizing monoclonal antibodies directed against hog cholera virus (HCV) precipitated two HCV-encoded glycoproteins, HCV gp55 and HCV gp33. Immunoassay with bacterial fusion proteins and Western immunoblotting with extracts from infected cells revealed that the antibodies recognized only HCV gp55. Coprecipitation of HCV gp33 was shown to be due to intermolecular disulfide bridges. One of the antibodies also reacted with the major glycoprotein of another pestivirus, bovine viral diarrhea virus (BVDV). The analogous BVDV glycoproteins exhibited a distribution of cysteine residues which was almost identical to that of HCV gp55 and gp33. The two BVDV glycoproteins were also linked by disulfide bridges.
authors.affiliation: Federal Research Centre for Virus Diseases of Animals, Tübingen, Federal Republic of Germany.
authors.forename: E
authors.initials: E
authors.lastname: Weiland
authors.forename: R
authors.initials: R
authors.lastname: Stark
authors.forename: B
authors.initials: B
authors.lastname: Haas
authors.forename: T
authors.initials: T
authors.lastname: Rümenapf
authors.forename: G
authors.initials: G
authors.lastname: Meyers
authors.forename: H J
authors.initials: HJ
authors.lastname: Thiel
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western; D002460:Cell Line; D006692:Classical Swine Fever Virus / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D003545:Cysteine / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005944:Glucosamine / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology*; D008715:Methionine / Q000378:metabolism; D009500:Neutralization Tests; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000032:analysis; D013462:Sulfur Radioisotopes; D014316:Tritium; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D011993:Recombinant Fusion Proteins; D013462:Sulfur Radioisotopes; D014764:Viral Proteins; D014316:Tritium; D008715:Methionine; D003545:Cysteine; D005944:Glucosamine