Determination of a new anti-allergenic agent, 1-[4-[3-[4-[bis-(4-fluorophenyl)hydroxymethyl]-1-piperidinyl] propoxy]-3-methoxyphenyl]ethanone, and its active acidic metabolite in plasma by high-performance liquid chromatography.
High-performance liquid chromatographic (HPLC) methods were developed for the analysis of two compounds in a series of new antiallergenic agents, 1-[4-[3-[4-[bis(4-fluorophenyl)hydroxymethyl]-1-piperidinyl] propoxy]-3-methoxyphenyl]ethanone and its active acidic metabolite in plasma. The methods utilize ultraviolet or fluorescence detection, liquid-liquid extraction or solid-phase extraction and reversed-phase HPLC. The drugs were quantitated in samples from bioavailability studies performed in dogs. Calibrations were in the ng/ml concentration range for both compounds in plasma.
Greene
S V
SV
Drug Metabolism Department, A.H. Robins Company, Richmond, VA 23220-6609.
Krebs
H A
HA
Lemieux
R E
RE
Cheng
L K
LK
D000818:Animals; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D004285:Dogs; D006801:Humans; D006967:Hypersensitivity / Q000188:drug therapy*; D008832:Microchemistry; D010880:Piperidines / Q000097:blood* / Q000493:pharmacokinetics; D011786:Quality Control
D010880:Piperidines; C055541:AHR 5333