Use of benzyl 2-acetamido-2-deoxy-3-O-(2-O-methyl-beta-D-galactosyl)-beta- D-glucopyranoside [2'-O-methyllacto-N-biose I beta Bn] as a specific acceptor for GDP-fucose: N-acetylglucosaminide alpha(1----4)-L-fucosyltransferase.
A synthetic substrate, benzyl 2-acetamido-2-deoxy-3-O-(2-O-methyl-beta-D- galactopyranosyl)-beta-D-glucopyranoside, was demonstrated to be a specific acceptor for the Lewis blood group-specified alpha(1----4)-L-fucosyltransferase from human saliva and stomach mucosa. The fucosyl linkage of the product resulting from the use of this substrate isolated by paper chromatography was characterized by hydrolysis with specific alpha(1----3)/(1----4)-L- fucosidase. The product can be separated by adsorption onto the reverse-phase cartridge and recovered by one-step elution with methanol. The enzymatic properties of alpha(1----4)-L-fucosyltransferase from saliva and stomach mucosa have also been examined using this substrate.
Yazawa
S
S
Department of Legal Medicine, School of Medicine, Gunma University, Maebashi, Japan.
Madiyalakan
R
R
Jain
R K
RK
Shimoda
N
N
Matta
K L
KL
D055598:Chemical Phenomena; D002621:Chemistry; D004187:Disaccharides; D005647:Fucosyltransferases / Q000302:isolation & purification; D006154:Guanosine Diphosphate Fucose / Q000378:metabolism*; D006602:Hexosyltransferases / Q000302:isolation & purification; D006801:Humans; D008861:Microsomes / Q000032:analysis; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D012463:Saliva / Q000032:analysis; D013270:Stomach / Q000032:analysis
D004187:Disaccharides; D009702:Nucleoside Diphosphate Sugars; C065133:benzyl 2-acetamido-2-deoxy-3-O-(2-O-methyl-beta-galactosyl)-beta-glucopyranoside; D006154:Guanosine Diphosphate Fucose; D005647:Fucosyltransferases; D006602:Hexosyltransferases; C019818:3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase