title: Isolation and microsequence analysis of a novel form of neuromedin U from guinea pig small intestine.
abstract: A multidimensional chromatographic regimen has been used to isolate and purify a peptide showing immunoreactivity for neuromedin U from guinea pig small intestine. Microsequence Edman N-terminal analysis and C-terminal analysis by enzymatic digestion showed this peptide to be a nonapeptide with the following sequence: H-Gly-Tyr-Phe-Leu-Phe-Arg-Pro-Arg-Asn-NH2. The C-terminal octapeptide of this sequence is the same as porcine NMU-8, and the C-terminal heptapeptide is identical to rat NMU(17-23).
authors.affiliation: Department of Anatomy and Histology, School of Medicine, Flinders University, Bedford Park, S.A., Australia.
authors.forename: R
authors.initials: R
authors.lastname: Murphy
authors.forename: C A
authors.initials: CA
authors.lastname: Turner
authors.forename: J B
authors.initials: JB
authors.lastname: Furness
authors.forename: L
authors.initials: L
authors.lastname: Parker
authors.forename: A
authors.initials: A
authors.lastname: Giraud
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002850:Chromatography, Gel; D006168:Guinea Pigs; D007421:Intestine, Small / Q000032:analysis*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000302:isolation & purification; D011863:Radioimmunoassay; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013552:Swine
chemical_list: D009479:Neuropeptides; C056900:neuromedin U