Biological activity of insulin-like growth factor-I purified from chicken serum.
This study describes a rapid purification of insulin-like growth factor-I from chicken serum and the immunological, biological and receptor binding activity of the peptide. It was purified after initial extraction, by cation exchange chromatography, hydrophobic interaction chromatography and reverse phase chromatography up to 1.4 x 10(6)-fold with an overall yield ranging from 10-30%. The N-terminal amino acid sequence was the same as predicted from the nucleotide sequence of a chicken IGF-I cDNA and the partial sequence obtained from a previously reported purification. The material was both immunologically and biologically active. It had a 50% potency compared to human IGF-I in a radioimmunoassay using an antiserum raised against human IGF-I, stimulated the incorporation of [3H]-thymidine into DNA in cultured chick embryo myoblasts with a half-maximum effective dose of 5 ng/ml and displaced [125I]-labelled human IGF-I and IGF-II from binding sites in microsomal membranes prepared from both the chicken liver and the lactating rabbit mammary gland in a dose dependent manner.
Armstrong
D G
DG
Department of Molecular Endocrinology, AFRC Institute of Animal Physiology and Genetics Research, Roslin, Midlothian, U.K.
Duclos
M J
MJ
Goddard
C
C
D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D005260:Female; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D008861:Microsomes / Q000187:drug effects; D008862:Microsomes, Liver / Q000187:drug effects; D008969:Molecular Sequence Data; D011817:Rabbits; D013002:Somatomedins / Q000494:pharmacology*
D013002:Somatomedins; D007334:Insulin-Like Growth Factor I