title: Effect of renal replacement therapy on cellular cytokine production in patients with renal disease.
abstract: To evaluate the effect of renal replacement therapy on cellular cytokine production in patients with renal disease, we studied interleukin 1 (IL-1) and interleukin-2 (IL-2) production by target cells stimulated with supernatants from cultured peripheral blood mononuclear cells from patients with end-stage renal disease, who were treated with hemodialysis and continuous ambulatory peritoneal dialysis (CAPD) before and after hemodialysis or a peritoneal dialysate exchange, compared to normal subjects used as time controls, and patients with chronic renal insufficiency. Initial cellular IL-2 production was increased in patients with hemodialysis, compared to normal controls. The ratio of pretreatment cellular IL-2 and IL-1 production was increased in all patient groups compared to normal subjects. Both hemodialysis and CAPD treatments resulted in increased cellular IL-1 (51.7%, P less than 0.007, and 42.8%, P less than 0.002, respectively) and IL-2 production (50.5%, P less than 0.015, and 33.3%, P less than 0.0008, respectively). The hemodialysis group had significantly higher cellular IL-2 production compared with normal controls' after treatment. After treatment, the IL-2/IL-1 ratio remained elevated in all groups with renal disease compared with normal subjects. We conclude patients with renal disease have an abnormal functional relationship between IL-1 and IL-2, characterized by increased IL-2 production per level of IL-1, unaffected by type or presence of renal replacement therapy.
authors.affiliation: Department of Medicine, Rita Gusack Laboratory, George Washington University Medical Center, Washington, D.C.
authors.forename: P L
authors.initials: PL
authors.lastname: Kimmel
authors.forename: T M
authors.initials: TM
authors.lastname: Phillips
authors.forename: E
authors.initials: E
authors.lastname: Phillips
authors.forename: J P
authors.initials: JP
authors.lastname: Bosch
mesh_terms: D001681:Biological Assay; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000096:biosynthesis*; D007676:Kidney Failure, Chronic / Q000378:metabolism / Q000628:therapy*; D008875:Middle Aged; D010531:Peritoneal Dialysis, Continuous Ambulatory; D006435:Renal Dialysis
chemical_list: D007375:Interleukin-1; D007376:Interleukin-2