[Contents of calcium, phosphorus and aluminum in central nervous system, liver and kidney of rabbits with experimental atherosclerosis--scavenger effects of vinpocetine on the deposition of elements].
The aims in this study were designed to clarify the contents of calcium (Ca), phosphorus (P) and aluminum (Al) in central nervous system (CNS), liver and kidney of rabbits with atherosclerosis experimentally induced by cholesterol-rich diet, and investigate scavenger effect of 14-ethoxycarbonyl-(3 alpha, 16 alpha-ethyl)-14,15-eburnamenine (vinpocetine) on the deposition of these elements in CNS and soft tissues of experimental atherosclerosis. Sixteen male rabbits were divided into 4 groups. Each group was fed with standard diet (Group A), standard diet containing 1.5% cholesterol (Group B), standard diet containing 1.5% cholesterol plus oral administration of 3 mg/kg/day vinpocetine (Group C), and standard diet containing 1.5% cholesterol plus administration of 10 mg/kg/day vinpocetine (Group D). After 3 months' feeding, experimental atherosclerosis was produced with a modified method of Kritchevsky et al in rabbits of Groups B, C and D. Blood was collected by cardiocentesis under the anesthesia of ether and then rabbits sacrificed to remove CNS and other tissues. The blood was stood for 1 hour at room temperature and separated by centrifugation at 3000 rpm for 10 min to determine serum total cholesterol, phospholipids, HDL-cholesterol, peroxide lipid, NEFA and calcium levels. Ca, P and Al contents in the frontal lobe, pons, cerebellum, spinal cord, liver and kidney were determined by neutron activation analysis. Ca contents of CNS, liver and kidney in Group B significantly increased than those of Group A (p less than 0.01), and significantly decreased in Groups C and D compared with those of Group B (p less than 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
Yasui
M
M
Department of Laboratory Medicine, Wakayama Medical College, Japan.
Yano
I
I
Ota
K
K
Oshima
A
A
D000535:Aluminum / Q000378:metabolism*; D000818:Animals; D001161:Arteriosclerosis / Q000097:blood / Q000378:metabolism* / Q000517:prevention & control; D002118:Calcium / Q000378:metabolism*; D002490:Central Nervous System / Q000378:metabolism*; D008076:Cholesterol, HDL / Q000097:blood; D007668:Kidney / Q000378:metabolism*; D008054:Lipid Peroxides / Q000097:blood; D008099:Liver / Q000378:metabolism*; D008297:Male; D010726:Phosphodiesterase Inhibitors; D010758:Phosphorus / Q000378:metabolism*; D011817:Rabbits; D014748:Vinca Alkaloids / Q000494:pharmacology*
D008076:Cholesterol, HDL; D008054:Lipid Peroxides; D010726:Phosphodiesterase Inhibitors; D014748:Vinca Alkaloids; D010758:Phosphorus; C013983:vinpocetine; D000535:Aluminum; D002118:Calcium