Developmental incompatibility between cell nucleus and cytoplasm as revealed by nuclear transplantation experiments in teleost of different families and orders.
Teleosts from different families and orders were used as materials for nuclear transplantation experiments. (1) The nuclei of goldfish (Carassius auratus, family Cyprinidae, order Cypriniformes) were transplanted into the enucleated egg cytoplasm of loach (Paramisgurnus dabryanus, family Cobitidae, order Cypriniformes) and vice-versa. (2) The nuclei of Tilapia (oreochromis nilotica, order Perciformes) were transplanted into the enucleated egg cytoplasm of goldfish (Carassius auratus, order Cypriniformes). The chromosome number of the nucleus donor fish is different from that of the cytoplasmic recipient fish in each of the two combinations. In the first case, only a few early nucleo-cytoplasmic hybrid (NCH) larval fish were obtained in each combination. In second case, even though a high percentage of NCH blastulas were also obtained, the majority of them died at the same developmental stage, except a few which survived until early gastrula stage. The examination of the metaphase chromosome figures of the NCH blastulas or embryos obtained in all three combinations indicated that they were of nucleus-donor type. The developmental rates of all the NCH eggs were similar to those of cytoplasmic-recipient type. Scanning electronmicroscopy examination showed that the morphology of NCH blastula cells, which were obtained from the combination of Tilapia nucleus and goldfish cytoplasm, manifested obviously abnormal features and the cells were arrested at different stages of cell disintegration. Two-dimension polyacrylamide gel electrophoretograms of the homogenates of Tilapia, goldfish and their NCH blastula cells showed that the protein synthetic pattern of NCH blastula was similar to that of Tilapia nucleus type. The results of experiments which failed to obtain NCH adult fish in all three combinations can be explained as a result of developmental incompatibility between the donor nucleus and the enucleated recipient egg cytoplasm, which were from distantly related fish species. And the chromosome numbers of all the component fish of the three combinations which were examined in the experiment and shown to be quite different from each other in the tested fish, should not be overlooked as one of the essential factors causing the developmental incompatibility in NCH fish in this experiment.
Yan
S Y
SY
Institute of Developmental Biology, Academia Sinica, Beijing, China.
Tu
M
M
Yang
H Y
HY
Mao
Z G
ZG
Zhao
Z Y
ZY
Fu
L J
LJ
Li
G S
GS
Huang
G P
GP
Li
S H
SH
Jin
G Q
GQ
D000818:Animals; D001755:Blastocyst / Q000502:physiology / Q000648:ultrastructure; D002467:Cell Nucleus / Q000502:physiology / Q000648:ultrastructure; D003531:Cypriniformes / Q000033:anatomy & histology / Q000196:embryology; D003593:Cytoplasm / Q000502:physiology* / Q000648:ultrastructure; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005306:Fertilization; D005399:Fishes / Q000033:anatomy & histology / Q000196:embryology*; D006054:Goldfish / Q000033:anatomy & histology / Q000196:embryology; D008297:Male; D008855:Microscopy, Electron, Scanning; D053652:Nuclear Transfer Techniques; D010063:Ovum / Q000648:ultrastructure*; D010473:Perciformes / Q000033:anatomy & histology / Q000196:embryology