Incorporation of the reovirus M cell attachment protein into small unilamellar vesicles: incorporation efficiency and binding capability to L929 cells in vitro.
Neutral phosphatidylcholine/cholesterol (10 : 3) liposomes with the reovirus M cell attachment protein sigma 1 were made by means of detergent dialysis method. An immunological evaluation method revealed an incorporation efficiency (pg protein/microgram lipid) of 314. Binding studies with mouse fibroblasts (L929 cells) yielded a 10 fold improvement of uptake of coated liposomes compared to uncoated liposomes. Competition studies with reovirus serotype 3 demonstrated that coated liposomes were capable of binding to the reovirus receptor. In vitro incubation of rat Peyer's patches with either coated or uncoated liposomes resulted in a 10-20 fold higher uptake of the coated liposomes. These results suggest that selective adherence of a carrier system to M cells may facilitate delivery of carrier contents to mucosal underlying lymphoid tissue, thereby enhancing immune response.
Rubas
W
W
Department of Pharmacy, Swiss Federal Institute of Technology ETH, Zürich.
Banerjea
A C
AC
Gallati
H
H
Speiser
P P
PP
Joklik
W K
WK
D000818:Animals; D036022:Capsid Proteins; D004337:Drug Carriers; D007739:L Cells; D008081:Liposomes; D012089:Mammalian orthoreovirus 3; D051379:Mice; D010581:Peyer's Patches / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011991:Receptors, Virus / Q000378:metabolism*; D012087:Reoviridae; D014764:Viral Proteins / Q000008:administration & dosage* / Q000378:metabolism
D036022:Capsid Proteins; D004337:Drug Carriers; D008081:Liposomes; D011991:Receptors, Virus; D014764:Viral Proteins; C046176:reovirus type 3 receptor; C031728:sigma 1 protein, reovirus