A new streptomycin-resistance plasmid from Staphylococcus hyicus and its structural relationship to other staphylococcal resistance plasmids.
A small plasmid of 4.4 kb encoding resistance to streptomycin (Smr) was detected in a multiresistant Staphylococcus hyicus culture from a piglet with exudative epidermitis. The plasmid-encoded properties were determined by interspecies protoplast transformation experiments. This plasmid was further characterised by restriction endonuclease analysis and a preliminary restriction map was constructed. The plasmid from S. hyicus that conferred streptomycin resistance was designated as pSAI-1. It showed some structural homology with the streptomycin-chloramphenicol resistance plasmid pSK68 from S. aureus of human origin. The MIC of streptomycin in resistance mediated by pSAI-1 was about 10 times higher than the MICs in resistance mediated by Smr plasmids from human S. aureus strains.
Schwarz
S
S
Institut für Bakteriologie und Immunologie, Justus Liebig-Universität, Giessen, FRG.
Blobel
H
H
D000818:Animals; D004352:Drug Resistance, Microbial / Q000235:genetics; D005819:Genetic Markers; D006801:Humans; D008826:Microbial Sensitivity Tests; D010957:Plasmids; D015183:Restriction Mapping; D013203:Staphylococcal Infections / Q000188:drug therapy; D013210:Staphylococcus / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D013307:Streptomycin / Q000494:pharmacology*; D013552:Swine
D005819:Genetic Markers; D013307:Streptomycin