An improved procedure for derivatization of controlled-pore glass beads for solid-phase oligonucleotide synthesis.
A simplified and economical method for the attachment of 2'-deoxyribo, ribo and arabinonucleosides onto long-chain alkylamidopropanoic acid controlled-pore glass (LCAAP-CPG, P-3) is described. In this procedure, 5'-O-tritylated nucleosides are coupled directly to LCAAP-CPG in excellent yields using 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (DEC) as coupling reagent. The conventional and time-consuming preparation of nucleoside-3'-O-succinates is no longer required.
Damha
M J
MJ
Department of Chemistry, Erindale College, University of Toronto, Mississauga, Ontario, Canada.
Giannaris
P A
PA
Zabarylo
S V
SV
D000089:Acetic Anhydrides; D001087:Arabinonucleosides; D055598:Chemical Phenomena; D002621:Chemistry; D003853:Deoxyribonucleosides; D015226:Dinucleoside Phosphates; D005898:Glass; D007700:Kinetics; D008722:Methods; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis*; D010416:Pentachlorophenol; D012263:Ribonucleosides
D000089:Acetic Anhydrides; D001087:Arabinonucleosides; D003853:Deoxyribonucleosides; D015226:Dinucleoside Phosphates; D009841:Oligonucleotides; D010416:Pentachlorophenol; D012263:Ribonucleosides; C015772:CpG dinucleotide; C031800:acetic anhydride