Evidence for probenecid-sensitive organic anion transporters on polarized thyroid cells in culture.
Epithelial thyroid cells in primary cultures loaded with BCECF/AM rapidly released the impermeant fluorescent dye BCECF (bis(carboxyethyl)carboxyfluorescein) in the incubation medium. Cells organized into follicles rapidly cleared BCECF (80% within 10 min) whereas fluorescence microscopy did not show any fluorescence in the follicular cavity. Cells organized into monolayers on plastic exported BCECF into the medium (70% within 40 min) whereas fluorescence microscopy showed intense fluorescence under the domes. BCECF efflux was blocked by probenecid, one of the known inhibitors of organic anion transporters, with similar efficiency in both structures. Maximal and half-maximal effects were respectively observed for 5 mM and 0.4 mM probenecid. The polarity of BCECF efflux was studied by using monolayers on collagen-coated Nuclepore filters: 85% of BCECF released was found in the basal compartment and 15% in the apical compartment. These findings suggested that thyroid cells in culture expressed a transport mechanism for the anionic form of BCECF. Furthermore, the observed activation of the Na+/H+ exchanger by probenecid suggested that the presence of this blocker did not overcome problems arising in the use of BCECF as intracellular pH indicator for thyroid cells.
Gérard
C
C
Faculté de Médecine Nord, INSERM, Unité U 270, Marseille, France.
Boudier
J A
JA
Mauchamp
J
J
Verrier
B
B
D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D027321:Anion Transport Proteins; D002352:Carrier Proteins / Q000378:metabolism*; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007700:Kinetics; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D011339:Probenecid / Q000494:pharmacology*; D012964:Sodium / Q000378:metabolism*; D013552:Swine; D013961:Thyroid Gland / Q000187:drug effects / Q000378:metabolism*
D027321:Anion Transport Proteins; D002352:Carrier Proteins; D005452:Fluoresceins; D005456:Fluorescent Dyes; D000643:Ammonium Chloride; C043829:2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein; D012964:Sodium; D011339:Probenecid