Description of a simple method to study some aspects of the pathogenesis of arteriosclerosis by perfusion of arterial segments.
A perfusion apparatus is described which permits the perfusion of arterial segments of about 5 cm in length. It works under physiological conditions. During the perfusion, temperature and pressure constancy or pressure variation, respectively, pulsation, sufficient oxygen supply, and high sterility are guaranteed. Hitherto, the duration of the perfusion has been extended to 10 h. After this time the endothelium was well preserved. The method shall be used for the study of some problems in arteriosclerosis research.
Krautschick
I
I
Institute of Pathological Anatomy, Karl Marx University Leipzig, GDR.
Künnert
B
B
Meier
T
T
D000818:Animals; D001012:Aorta, Abdominal; D001161:Arteriosclerosis / Q000209:etiology* / Q000503:physiopathology; D004730:Endothelium, Vascular; D006801:Humans; D007260:Infusion Pumps; D010477:Perfusion / Q000379:methods*; D012039:Regional Blood Flow; D013552:Swine