The neuropeptides vasoactive intestinal polypeptide, peptide histidine isoleucine and neuropeptide Y modulate [3H]noradrenaline release from sympathetic nerves in the choroid plexus.
The neurotransmitter peptides vasoactive intestinal polypeptide (VIP), peptide histidine isoleucine (PHI) and neuropeptide Y (NPY) are located in nerve fibers supplying the pig choroid plexus, which receives an abundant sympathetic innervation. We characterized the release of [3H]noradrenaline ([3H]NA) from this tissue elicited by electrical field stimulation and studied the effects of the above-mentioned peptides on this release. The release of [3H]NA was found to be almost exclusively of neurogenic origin, despite there being a marked non-neuronal uptake of [3H]NA into the epithelium of the choroid plexus. NPY significantly decreased the release of [3H]NA by approximately 10% while VIP and PHI enhanced release by up to 25 and 35%, respectively, indicating a possible synergistic role of the two latter peptides and NA in the choroid plexus.
Nilsson
C
C
Department of Medical Cell Research, University of Lund, Sweden.
Kannisto
P
P
Lindvall-Axelsson
M
M
Owman
C
C
Rosengren
E
E
D000818:Animals; D002831:Choroid Plexus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004558:Electric Stimulation; D009478:Neuropeptide Y / Q000494:pharmacology*; D009638:Norepinephrine / Q000378:metabolism*; D010451:Peptide PHI / Q000494:pharmacology*; D011188:Potassium / Q000494:pharmacology; D013552:Swine; D013564:Sympathetic Nervous System / Q000378:metabolism*; D013779:Tetrodotoxin / Q000494:pharmacology; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
D009478:Neuropeptide Y; D010451:Peptide PHI; D014660:Vasoactive Intestinal Peptide; D013779:Tetrodotoxin; D011188:Potassium; D009638:Norepinephrine