title: Type C Niemann-Pick disease: documentation of abnormal LDL processing in lymphocytes.
abstract: Type C Niemann-Pick disease (NPC) is an autosomal recessive neurovisceral storage disorder in which defective intracellular cholesterol processing has been demonstrated in fibroblasts from NPC patients and obligate heterozygotes. In the present paper, the ability to esterify LDL-cholesterol was examined in cultured lymphocytes from 8 NPC patients, 8 obligate heterozygotes and 8 controls. Cholesteryl ester synthesis was 8% (+/- 5%) and 45% (+/- 16%) of controls in homozygous and heterozygous cell lines, respectively. Histochemical and electron microscopic examinations confirmed that this biochemical lesion was associated with abnormal intracellular accumulation of unesterified cholesterol in mutant lymphocytes. These results demonstrate that measurement of cholesterol esterification in cultured lymphocytes offers a quick and reliable means of confirming the diagnosis of NPC and that these cells may be useful for probing the primary molecular lesion of NPC.
authors.affiliation: Developmental and Metabolic Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
authors.forename: C E
authors.initials: CE
authors.lastname: Argoff
authors.forename: C R
authors.initials: CR
authors.lastname: Kaneski
authors.forename: E J
authors.initials: EJ
authors.lastname: Blanchette-Mackie
authors.forename: M
authors.initials: M
authors.lastname: Comly
authors.forename: N K
authors.initials: NK
authors.lastname: Dwyer
authors.forename: A
authors.initials: A
authors.lastname: Brown
authors.forename: R O
authors.initials: RO
authors.lastname: Brady
authors.forename: P G
authors.initials: PG
authors.lastname: Pentchev
mesh_terms: D002451:Cell Compartmentation; D002788:Cholesterol Esters / Q000378:metabolism; D008078:Cholesterol, LDL / Q000378:metabolism*; D005372:Filipin / Q000032:analysis; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism*; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D009542:Niemann-Pick Diseases / Q000235:genetics / Q000378:metabolism*
chemical_list: D002788:Cholesterol Esters; D008078:Cholesterol, LDL; D008077:Lipoproteins, LDL; D005372:Filipin