title: Novobiocin-resistance sequences from the novobiocin-producing strain Streptomyces niveus.
abstract: Two distinct DNA sequences expressing novobiocin resistance in Streptomyces lividans were cloned from the novobiocin-producing species Streptomyces niveus. Clone pGL101 (5kb) conferred resistance to 50 micrograms ml-1 novobiocin, whereas clones pGL102 and pGL103, which carry the same 6.5kb insert but in opposite orientations, expressed resistance to 150 micrograms ml-1. The cloned inserts from pGL101 and pGL103 failed to hybridize with each other or with the cloned novobiocin-resistant gyrB sequence from Streptomyces sphaeroides. Both probes hybridized strongly with DNA from the novobiocin-producing species S. niveus and S. sphaeroides but no hybridization (pGL103) or very weak hybridization (pGL101) was detected with DNA from the non-producing species S. lividans, Streptomyces griseus and Streptomyces antibioticus. S. niveus contains at least three novobiocin-resistance determinants with the pGL101 and pGL103 cloned sequences specific for novobiocin-producing strains of Streptomyces.
authors.affiliation: Department of Genetics and Microbiology, University of Liverpool, Donnan Laboratories, UK.
authors.forename: J I
authors.initials: JI
authors.lastname: Mitchell
authors.forename: P G
authors.initials: PG
authors.lastname: Logan
authors.forename: K E
authors.initials: KE
authors.lastname: Cushing
authors.forename: D A
authors.initials: DA
authors.lastname: Ritchie
mesh_terms: D015139:Blotting, Southern; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis; D004352:Drug Resistance, Microbial / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D009675:Novobiocin / Q000494:pharmacology*; D015183:Restriction Mapping; D013302:Streptomyces / Q000187:drug effects / Q000235:genetics*; D013329:Structure-Activity Relationship
chemical_list: D004269:DNA, Bacterial; D009675:Novobiocin