Selective and rapid solubilization of the microbial membrane enzyme aldehyde dehydrogenase.
An improved solubilization procedure for the membrane-bound quinoprotein aldehyde dehydrogenase from Acetobacter rancens CCM 1774 was established. After the first solubilization of membrane enzymes by Brij 35 which provided important extraction of membrane proteins other than aldehyde dehydrogenase, the application of Trition X-100 resulted in an almost 20-fold purification of quinoprotein aldehyde dehydrogenase. The optimal solubilization was closely connected with definite detergent/protein ratios.
Hommel
R
R
Bereich Biochemie der Sektion Biowissenschaften der Karl-Marx-Universität Leipzig, DDR.
Kleber
H P
HP
D000091:Acetobacter / Q000201:enzymology*; D000444:Aldehyde Dehydrogenase / Q000302:isolation & purification*; D002462:Cell Membrane / Q000201:enzymology; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology
D011092:Polyethylene Glycols; D017830:Octoxynol; D000444:Aldehyde Dehydrogenase