1H NMR studies of oxidized high-potential iron protein from Chromatium vinosum. Nuclear Overhauser effect measurements.
1H nuclear Overhauser effect experiments on the isotropically shifted signals of oxidized Chromatium vinosum HiPIP have been used to identify the four beta-CH2 geminal couples of the cysteine ligands. A partial assignment to individual residues has been proposed from a computer graphics analysis of the X-ray structure. Tentative assignments of other resonances are discussed.
Cowan
J A
JA
Evans Laboratory of Chemistry, Ohio State University, Columbus 43210.
Sola
M
M
D001426:Bacterial Proteins; D002844:Chromatium; D003545:Cysteine; D007506:Iron-Sulfur Proteins; D009682:Magnetic Resonance Spectroscopy; D008667:Metalloproteins; D015394:Molecular Structure; D010084:Oxidation-Reduction; D045322:Photosynthetic Reaction Center Complex Proteins; D014961:X-Ray Diffraction
D001426:Bacterial Proteins; D007506:Iron-Sulfur Proteins; D008667:Metalloproteins; D045322:Photosynthetic Reaction Center Complex Proteins; C007943:high potential iron-sulfur protein; D003545:Cysteine