Persistence of parvovirus H-1 DNA in human B- and T-lymphoma cells.
Persisting DNA of parvovirus H-1 could be demonstrated in cells of two human lymphoma cell lines, the Burkitt lymphoma cell line BL2 and the T-cell leukemia cell line Jurkat which survived infection with parvovirus H-1. Persistence of H-1 DNA rendered the cells resistant to a second H-1 infection. This resistance to H-1 superinfection persisted even after loss of H-1 DNA occurring after approximately 150-200 cell generations. Resistance to H-1 superinfection was accompanied by reduced uptake of infectious particles and by a block of H-1 DNA replication. This suggests that persistent H-1 infection leads to modifications of cellular functions involved in the permissivity for H-1.
Faisst
S
S
Institut für Virusforschung, Deutsches Krebsforschungszentrum, Heidelberg, F.R.G.
Bartnitzke
S
S
Schlehofer
J R
JR
zur Hausen
H
H
D002051:Burkitt Lymphoma / Q000235:genetics / Q000382:microbiology*; D002472:Cell Transformation, Viral; D004261:DNA Replication; D004279:DNA, Viral / Q000378:metabolism*; D005784:Gene Amplification; D006801:Humans; D007700:Kinetics; D015448:Leukemia, B-Cell / Q000235:genetics / Q000382:microbiology*; D015458:Leukemia, T-Cell / Q000235:genetics / Q000382:microbiology*; D010321:Parvoviridae / Q000235:genetics* / Q000254:growth & development; D015163:Superinfection / Q000235:genetics*; D014407:Tumor Cells, Cultured; D014779:Virus Replication / Q000235:genetics
D004279:DNA, Viral