A 21 KDa protein resides in the nucleosomes of micrococcal nuclease sensitive liver chromatin of Atlantic salmon treated with estradiol.
Atlantic salmon was treated with 17-beta-estradiol to induce the process of vitellogenesis in liver. When the isolated liver nuclei were incubated with micrococcal nuclease at increasing enzyme/DNA ratios a 21 KDa protein appeared in the nuclease sensitive chromatin, the S-fraction. After HPLC gel filtration the 21 KDa protein resided with the oligo- and mononucleosomes. The S-fraction contained vitellogenin gene sequences at low nuclease/DNA ratios. The sequences were detectable also in the mononucleosomes derived from the S-fraction. After hormone treatment the vitellogenin gene exposed a higher sensitivity to micrococcal nuclease than the hormone-untreated controls. The results indicate that the 21 KDa protein took part in the hormone-mediated changes in gene expression by modulating the structure of estradiol responsive chromatin domains including the vitellogenin gene.
Waters
S
S
Wenner-Gren Institute for Experimental Biology, University of Stockholm, Sweden.
von der Decken
A
A
D000818:Animals; D002843:Chromatin / Q000032:analysis* / Q000187:drug effects / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression; D008099:Liver / Q000032:analysis*; D008836:Micrococcal Nuclease / Q000378:metabolism; D009687:Nuclear Proteins / Q000032:analysis*; D009707:Nucleosomes / Q000032:analysis*; D012474:Salmon / Q000378:metabolism*; D014819:Vitellogenins / Q000235:genetics
D002843:Chromatin; D009687:Nuclear Proteins; D009707:Nucleosomes; D014819:Vitellogenins; D004958:Estradiol; D008836:Micrococcal Nuclease