Simultaneous determination of 2-deoxy-D-glucose and D-glucose in rat serum by high-performance liquid chromatography with post-column fluorescence derivatization.
A simple high-performance liquid chromatographic method for the determination of 2-deoxy-D-glucose and D-glucose in rat serum is described; this method is based on a post-column fluorescence derivatization. The sugars are automatically converted into fluorescent derivatives by reaction with meso-1,2-bis(4-methoxyphenyl)ethylenediamine in an alkaline medium after their separation on a strong anion exchanger column (TSK gel Sugar AXG). The detection limits (S/N = 3) for 2-deoxy-D-glucose and D-glucose in rat serum are 0.52 and 0.56 nmol/ml, respectively.
Umegae
Y
Y
Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Nohta
H
H
Ohkura
Y
Y
D000818:Animals; D001786:Blood Glucose / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003837:Deoxy Sugars / Q000097:blood*; D003847:Deoxyglucose / Q000097:blood*; D005260:Female; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence
D001786:Blood Glucose; D003837:Deoxy Sugars; D003847:Deoxyglucose