Determination of the active metabolite of molsidomine in human plasma by reversed-phase high-performance liquid chromatography.
A reversed-phase high-performance liquid chromatographic method, with ultraviolet detection, is proposed for the plasma determination of SIN-1, the active metabolite of molsidomine, which involves propoxycarbonyl derivatization. The internal standard is the ethoxycarbonyl derivative of SIN-1 (i.e. molsidomine). Derivatization and extraction are each performed in one step (2 min) with 70% yield. The nature of a by-product is discussed. The method provides rapid elution (less than 15 min), linearity over the range 0.4-200 ng/ml, day-to-day precision between 2.5 and 11.3% and a limit of determination of 0.5 ng/ml. This method is also suitable for the simultaneous determination of molsidomine and SIN-1. In this case the internal standard is an ethoxycarbonyl derivative of a piperazino-3-sydnonimine, a SIN-1 analogue.
Dutot
C
C
Département de Biochimie et de Pharmacologie, Centre Hospitalier de Versailles, Le Chesnay, France.
Moreau
J
J
Cordonnier
P
P
Spreux-Varoquaux
O
O
Klein
C
C
Ostrowski
J
J
Advenier
C
C
Gärtner
W
W
Pays
M
M
D000959:Antihypertensive Agents / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008875:Middle Aged; D008981:Molsidomine / Q000031:analogs & derivatives* / Q000097:blood* / Q000493:pharmacokinetics
D000959:Antihypertensive Agents; C002385:linsidomine; D008981:Molsidomine