[The chromatin structure of transcriptionally active and inactive repetitive nucleotide sequences].
The chromatin structure of a transcriptionally active gene coding for 18S-ribosomal RNA in rat cells differing in transcriptional activity (cerebellum neurons and thymocytes) was studied. In the presence of 3 mM MgCl2 nuclease digestion with DNAase I or DNAase II and subsequent blot hybridization analysis revealed that this gene has a completely decondensed chromatin structure in neuronal nuclei. Under the same conditions the 18S ribosomal gene has a partially unfolded chromatin structure in thymocyte nuclei according to the earlier described "nuclease criterion". At 3 mM MgCl2 inactive chromatin of the repeating genomic DNA element has a compact structure which is characterized by a dinucleosomal periodicity of fragmentation with the DNAases and has the same pattern of decompactization after MgCl2 concentration reduction as total chromatin of pigeon erythrocyte nuclei does.
Kukushkin
A N
AN
Pospelov
V A
VA
D000818:Animals; D002843:Chromatin / Q000378:metabolism* / Q000648:ultrastructure; D010856:Columbidae; D004247:DNA / Q000235:genetics; D003851:Deoxyribonucleases / Q000378:metabolism; D009690:Nucleic Acid Conformation; D011487:Protein Conformation; D012335:RNA, Ribosomal / Q000235:genetics*; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic
D002843:Chromatin; D012335:RNA, Ribosomal; D012337:RNA, Ribosomal, 18S; D004247:DNA; D003851:Deoxyribonucleases
Struktura khromatina transkriptsionno aktivnykh i neaktivnykh povtoriaiushchikhsia nukleotidnykh posledovatel'nosteĭ.