Isolation and analysis of the human MEKA gene encoding a retina-specific protein.
We have isolated and sequenced a human gene encoding photoreceptor cell-specific MEKA protein. The protein coding region was separated into three exons, which encoded 246 amino acid residues with a molecular weight of 28,311. The coding region of the human gene exhibited the homologies of 90.7% nucleotides and 88.5% amino acids with those of the bovine cDNA. Southern blot analysis revealed that the human MEKA gene has a single copy number. However, the anti-bovine MEKA stained both rod and cone photoreceptor cells in the human retina.
Watanabe
Y
Y
Department of Pharmacology, Kanazawa University, Japan.
Kawasaki
K
K
Miki
N
N
Kuo
C H
CH
D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D004247:DNA / Q000378:metabolism; D005136:Eye Proteins / Q000235:genetics*; D020555:GTP-Binding Protein Regulators; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010750:Phosphoproteins; D015183:Restriction Mapping; D012160:Retina / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid
C020932:11-cis-retinal-binding protein; D002352:Carrier Proteins; D005136:Eye Proteins; D020555:GTP-Binding Protein Regulators; D010750:Phosphoproteins; C065631:phosducin; D004247:DNA