Effect of cytochalasin D on the adhesion of a neuroblastoma x glioma cell line (NG108-15) to laminin and plastic substrates.
Adhesion of the neuronal cell surface to its underlying substrate plays an important role in neurite outgrowth in vitro. I have investigated the adhesive basis for neurite outgrowth in the presence of cytochalasin D, a disruptor of actin-containing microfilaments, and in the presence of vinblastine, a depolymerizer of microtubules. Scanning electron microscopy shows that cytochalasin D does not alter the branching configuration of filopodia on a laminin substrate, although processes are shorter and tapered distally in the presence of the drug. Using a standard attachment assay for the neuroblastoma x glioma cell line (NG108-15) I show that vinblastine does not influence attachment of NG108-15 cells to either plastic or laminin. Cytochalasin D-treated cells normally attach to high concentrations of a laminin substrate (20 micrograms/ml). However, when cell are seeded on a laminin substrate at lower concentrations (0.001-10 micrograms/ml), or on YIGSR, a fragment of laminin, cytochalasin D increases cell attachment. Cytochalasin D increases attachment in a dose-dependent manner when cells are seeded on plain polystyrene plastic, so that the number of cells attached to plastic in 1 microM cytochalasin D is similar to the number attached to laminin (20 micrograms/ml). Combining low concentrations of cytochalasin D and laminin results in greater attachment than with either agent alone. Mild trypsinization of the cell surface reduces the CD-enhanced attachment to plastic, indicating that a protein on the cell surface may be involved. The effect of cytochalasin D appears to be cell specific since cytochalasin D does not affect the attachment of a fibroblast cell line (NIH 3T3) to laminin and plastic.(ABSTRACT TRUNCATED AT 250 WORDS)
Luckenbill-Edds
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Department of Zoological and Biomedical Sciences, Ohio University, Athens 45701.
D000818:Animals; D006632:Histamine / Q000378:metabolism*; D007150:Immunohistochemistry; D009420:Nervous System / Q000166:cytology / Q000378:metabolism*; D010986:Platyhelminths / Q000166:cytology / Q000378:metabolism*; D013032:Sparganum / Q000166:cytology / Q000378:metabolism*; D013045:Species Specificity
D006632:Histamine