[A new method for determination of nicotinic acid in plasma].
A new and sensitive method for the detection of nicotinic acid in plasma is described. Human or rat plasma-samples from pharmacological or pharmacokinetic studies are lyophilized and extracted with methanol. After removal of the solvent the residue is converted with N-Methyl-N-trimethylsilyltrifluoracetamide to the trimethylsilylester of nicotinic acid, separated by vpc and detected with a mass selective detector via the fragment 180 amu. 6-Methyl-nicotinic acid is used as internal standard (fragment 194 amu). With this method a detection limit of 100 ng/ml plasma is reached.
Becker
B
B
Pharmakokinetische Abteilung der Fa. Merz + Co. GmbH & Co., Frankfurt/Main.
Hummel
K
K
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D009525:Niacin / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D014297:Trimethylsilyl Compounds / Q000032:analysis
D014297:Trimethylsilyl Compounds; D009525:Niacin
Ein neues Verfahren zur Bestimmung von Nikotinsäure im Plasma.