beta-globin dominant control region interacts differently with distal and proximal promoter elements.
We have studied the interaction between the dominant control region (DCR) and the promoter of the human beta-globin gene. Expression analysis in MEL cells has revealed that the DCR contains a number of elements capable of replacing the upstream (-250 to -100) erythroid-specific region of the promoter. The DCR strongly stimulates expression from a promoter possessing only a TATA box. However, this basic level of transcription is not induced upon erythroid differentiation of the cells. Mutational analysis of the minimal (-100, noninducible) promoter shows that only the combination of the DCR and the CAC/CCAAT elements provides erythroid-specific transcription. These regions act synergistically to produce full regulated expression during erythroid differentiation.
Antoniou
M
M
Laboratory of Gene Structure and Expression, National Institute for Medical Research, Mill Hill, London, UK.
Grosveld
F
F
D001483:Base Sequence; D002454:Cell Differentiation; D004252:DNA Mutational Analysis; D004912:Erythrocytes / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation; D005914:Globins / Q000235:genetics*; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D005914:Globins