Detection in non-erythroid cells of a factor with the binding characteristics of the erythroid cell transcription factor EF1.
The erythroid transcription factor erythroid factor-1 (EF1) plays a critical role in the transcription of erythroid-specific genes. Here we report the presence of a factor with the mobility and sequence-specific DNA-binding characteristics of EF1 at low abundance in a wide variety of non-erythroid cell types. This is the first report of an EF1-like activity in non-erythroid cells and indicates that this factor may play a role in the regulation of genes expressed in such cells.
Perkins
N D
ND
Institute of Cancer Research, Chester Beatty Laboratories, London, U.K.
Orchard
K H
KH
Collins
M L
ML
Latchman
D S
DS
Goodwin
G H
GH
D000818:Animals; D001667:Binding, Competitive; D002460:Cell Line; D002467:Cell Nucleus / Q000032:analysis; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D050981:Erythroid-Specific DNA-Binding Factors; D006367:HeLa Cells; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute; D051379:Mice; D013601:T-Lymphocytes / Q000032:analysis; D013724:Teratoma / Q000032:analysis; D014157:Transcription Factors / Q000032:analysis*; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D014157:Transcription Factors; D004247:DNA