Osteoarthrosis induced by intra-articular hydrogen peroxide injection and running load.
Osteoarthrosis of the rat knee joint was induced successfully by intra-articular injection of 2% hydrogen peroxide (H2O2) and running load. In the group receiving two injections of physiological saline with running load, no histological osteoarthrotic knees were developed up to 8 weeks after the injection. In the group receiving two 2% H2O2 injections and running load, advanced osteoarthrosis occurred in all knees 8 weeks after the first injection, and the Mankin's score of osteoarthrotic knees appeared to be higher than the group receiving two 2% H2O2 injections without running load (p less than 0.004, Kruskal-Wallis H test). In the rats receiving a single 2% H2O2 injection with running load and a vitamin E-enriched diet, the inhibitory effect of vitamin E on the development of osteoarthrosis was statistically significant compared to the regular diet group (p less than 0.05, Wilcoxon rank test).
Kaiki
G
G
Department of Orthopaedic Surgery, Toyama Medical and Pharmaceutical University, Japan.
Tsuji
H
H
Yonezawa
T
T
Sekido
H
H
Takano
T
T
Yamashita
S
S
Hirano
N
N
Sano
A
A
D000818:Animals; D004032:Diet; D006861:Hydrogen Peroxide; D007270:Injections, Intra-Articular; D008297:Male; D009994:Osmolar Concentration; D010003:Osteoarthritis / Q000139:chemically induced* / Q000000981:diagnostic imaging / Q000473:pathology; D011859:Radiography; D051381:Rats; D011919:Rats, Inbred Strains; D012420:Running; D012965:Sodium Chloride / Q000494:pharmacology; D014810:Vitamin E / Q000008:administration & dosage / Q000494:pharmacology
D014810:Vitamin E; D012965:Sodium Chloride; D006861:Hydrogen Peroxide