Microdissection of the fragile X region.
We have microdissected and cloned the region around the fragile site at Xq27.3 on the human X chromosome. All of the clones tested map to the Xq27-Xq28 region, and detailed mapping on a panel of somatic cell hybrids indicates that the microdissected library contains sequences derived from both sides of the fragile X mutation. Some of these clones give signals in rodent DNA. This library demonstrates the power of microdissection for the identification of potential coding sequences near a disease locus and provides a promising resource for the identification of the fragile X mutation.
MacKinnon
R N
RN
Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, England.
Hirst
M C
MC
Bell
M V
MV
Watson
J E
JE
Claussen
U
U
Ludecke
H J
HJ
Senger
G
G
Horsthemke
B
B
Davies
K E
KE
D000818:Animals; D015139:Blotting, Southern; D002871:Chromosome Banding; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D015342:DNA Probes; D005600:Fragile X Syndrome / Q000235:genetics*; D005784:Gene Amplification; D006801:Humans; D006822:Hybrid Cells; D007621:Karyotyping; D015183:Restriction Mapping; D012729:Sex Chromosome Aberrations / Q000235:genetics*; D014960:X Chromosome
D015342:DNA Probes