[Standardization of conditions for detecting the internal proteins of the influenza virus in studying their antigenic properties in solid-phase immunoenzyme analysis].
The influence of the conditions of adsorption and virion destruction by freezing-thawing and detergents on the detection of M1 and NP proteins of different influenza virus strains by solid-phase enzyme immunoassay with direct virion adsorption on polystyrene was studied. It was found that for the detection of M1 protein the optimal conditions included virion disruption with detergent and adsorption to polystyrene at 4 degrees C, and for NP protein disruption by freezing-thawing at adsorption to polystyrene at 37 degrees C. In the study of the antigenic properties of protein M1 of different influenza virus strains using monoclonal antibodies it was shown to be necessary, first, to achieve maximum detection of proteins and, second, to standardize the amount of the adsorbed antigen with polyclonal antibodies.
Schefer
J
J
Khristova
M L
ML
Busse
T L
TL
Sinnecker
R
R
Kharitonenkov
I G
IG
D000327:Adsorption; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D002213:Capsid / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards; D009980:Influenza A virus / Q000032:analysis* / Q000276:immunology; D013696:Temperature; D014758:Viral Core Proteins / Q000032:analysis* / Q000276:immunology; D014763:Viral Matrix Proteins / Q000032:analysis* / Q000276:immunology; D014771:Virion / Q000032:analysis / Q000276:immunology
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D014758:Viral Core Proteins; D014763:Viral Matrix Proteins
Standartizatsiia usloviĭ vyiavleniia vnutrennikh belkov virusa grippa pri issledovanii ikh antigennykh svoĭstv v tverdofaznom immunofermentnom analize.