In vitro transformation induces abnormal protein thiol levels in rat liver cells.
Cells from an established line of normal rat hepatocytes (IAR 20) were irradiated with 3 Gy gamma-radiation from a cobalt source to generate transformed clones. Cells from four transformed colonies were compared with the parent cell line by flow cytometry following staining with ortho-phthalaldehyde and propidium iodide to quantitate protein thiols and DNA respectively. Transformed cells exhibited an increased variability in cellular protein thiols which was most evident in G1 and S phase. The altered pattern of macromolecular thiol distribution implies early changes in redox state and/or modification of the amounts or types of sulphur-containing proteins in transformed cells.
Principe
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P
Department of Chemical Pathology, University College and Middlesex School of Medicine, London, UK.
Wilson
G D
GD
Riley
P A
PA
Slater
T F
TF
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D005434:Flow Cytometry; D005453:Fluorescence; D007399:Interphase; D008099:Liver / Q000032:analysis / Q000528:radiation effects*; D011506:Proteins / Q000032:analysis*; D051381:Rats; D013438:Sulfhydryl Compounds / Q000032:analysis*
D011506:Proteins; D013438:Sulfhydryl Compounds