title: cDNA cloning of rat liver 2,4-dienoyl-CoA reductase.
abstract: cDNA clones of 2,4-dienoyl-CoA reductase were isolated from rat liver cDNA libraries constructed in phages lambda gt11 and lambda gt10. Hybrid selected translation analysis revealed that 2,4-dienoyl-CoA reductase was translated as a polypeptide with a molecular weight of about 36,000, which was about 3,000 molecular weight units larger than mature reductase. Sequencing analysis revealed that the open reading frame encoded a polypeptide consisting of 335 amino acid residues (predicted molecular weight = 36,132), which contained an N-terminal extension peptide of 34 amino acid residues (presequence) in addition to the mature enzyme. Thus, 2,4-dienoyl-CoA reductase is synthesized as a larger precursor polypeptide, and the N-terminal extension peptide may be acting as the mitochondrial import signal.
authors.affiliation: Department of Pharmaceutical Sciences, Tohoku University Hospital, Miyagi, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Hirose
authors.forename: K
authors.initials: K
authors.lastname: Kamijo
authors.forename: T
authors.initials: T
authors.lastname: Osumi
authors.forename: T
authors.initials: T
authors.lastname: Hashimoto
authors.forename: M
authors.initials: M
authors.lastname: Mizugaki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D044943:Fatty Acid Desaturases / Q000235:genetics*; D008830:Microbodies / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D044925:Oxidoreductases Acting on CH-CH Group Donors; D011233:Precipitin Tests; D051381:Rats; D015183:Restriction Mapping
chemical_list: D004247:DNA; D044943:Fatty Acid Desaturases; D044925:Oxidoreductases Acting on CH-CH Group Donors; C100153:2,4-dienoyl-CoA reductase