title: The MURF3 gene of T. brucei contains multiple domains of extensive editing and is homologous to a subunit of NADH dehydrogenase.
abstract: Mitochondrial MURF3 transcripts of T. brucei are extensively edited by the addition and deletion of uridines. The editing creates potential initiation and termination codons and a continuous open reading frame. The predicted amino acid sequence has homology to a subunit of NADH dehydrogenase (ND7). ND7 is independently edited in two distinct domains, suggesting two editing initiation sites. Editing in the two domains is differentially regulated: the 5' domain is edited in both bloodstream and procyclic forms but the 3' domain is completely edited only in the bloodstream form. Two potential guide RNA (gRNA) coding sequences were identified in the same minicircle. One is complementary to edited sequence in the 5' domain, the other to edited sequence in the 3' domain.
authors.affiliation: Seattle Biomedical Research Institute, Seattle, Washington 98109-1651.
authors.forename: D J
authors.initials: DJ
authors.lastname: Koslowsky
authors.forename: G J
authors.initials: GJ
authors.lastname: Bhat
authors.forename: A L
authors.initials: AL
authors.lastname: Perrollaz
authors.forename: J E
authors.initials: JE
authors.lastname: Feagin
authors.forename: K
authors.initials: K
authors.lastname: Stuart
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003579:Cytochrome Reductases / Q000235:genetics*; D004270:DNA, Circular / Q000235:genetics; D015723:Gene Library; D005796:Genes; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics*; D015345:Oligonucleotide Probes; D012689:Sequence Homology, Nucleic Acid; D012984:Software; D014158:Transcription, Genetic; D014346:Trypanosoma brucei brucei / Q000235:genetics*
chemical_list: D004270:DNA, Circular; D046911:Macromolecular Substances; D015345:Oligonucleotide Probes; D003579:Cytochrome Reductases; D009245:NADH Dehydrogenase