Colonial morphology of tumour cells and susceptibility to cytolysis by tumour necrosis factor. The role of cellular fibronectin deposition in the extracellular matrix.
The tumour cell lines U937A and L929 form large, loosely packed colonies in vitro and can be killed by the cytokine tumour necrosis factor (TNF). In contrast, their TNF-resistant mutants U937A/R and L929/R form tightly packed colonies. Since cells which form loose colonies have increased metastatic potential it is important to understand the factors governing colonial morphology. To this end, we have compared the extracellular matrices (ECMs) of the 'loose' lines, U937A and L929 with their 'tight' mutants. By immunofluorescence, a polyvalent anti-U937A serum revealed a fibrillar network in the ECMs of the 'loose' lines which was absent in the 'tight'. On Western blotting of ECMs the antiserum detected an additional 300 kDa protein in the 'loose' lines which was subsequently shown to be cellular fibronectin. The four lines secreted comparable amounts of fibronectin and this was qualitatively indistinguishable between 'loose' and 'tight' cells by peptide mapping or lectin binding. It is concluded that the differences in colonial morphology are due to the 'tight' mutants' inability to incorporate fibronectin into the ECM.
Neale
M L
ML
Department of Medical Microbiology, University of Wales College of Medicine, Heath Park, Cardiff, UK.
Matthews
N
N
D001345:Autoradiography; D015153:Blotting, Western; D004351:Drug Resistance / Q000235:genetics; D005109:Extracellular Matrix / Q000032:analysis / Q000648:ultrastructure*; D005353:Fibronectins / Q000032:analysis*; D007797:Laminin / Q000032:analysis; D008970:Molecular Weight; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D005353:Fibronectins; D007797:Laminin; D014409:Tumor Necrosis Factor-alpha