title: Measurement of free human leukocyte elastase and human leukocyte elastase/alpha 1 proteinase inhibitor complexes by an enzyme-linked immunosorbent assay.
abstract: We report on an ELISA procedure for the quantitative analysis of total human leukocyte (PMN)-elastase, i.e., the simultaneous determination of (i) free (non-complexed) PMN-elastase and of (ii) PMN-elastase that is complexed to alpha 1 proteinase inhibitor. Simultaneous detection of both forms of PMN-elastase was achieved using a monoclonal antibody that recognizes a PMN-elastase-specific epitope present on both the uncomplexed and the complexed form of the enzyme. The test system described is reliable, easy to perform and permits the determination of total PMN-elastase in complex biological fluids such as plasma or seminal fluid. It is to be expected that this test system will be useful for investigations of human PMN-elastase in biological specimens obtained from both normal and pathological conditions.
authors.affiliation: Universitäts-Hautklinik, Heidelberg, F.R.G.
authors.forename: M D
authors.initials: MD
authors.lastname: Kramer
authors.forename: M
authors.initials: M
authors.lastname: Müller-Bardorff
authors.forename: M M
authors.initials: MM
authors.lastname: Simon
authors.forename: W
authors.initials: W
authors.lastname: Tilgen
authors.forename: E
authors.initials: E
authors.lastname: Schickel
authors.forename: D
authors.initials: D
authors.lastname: Petzoldt
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007962:Leukocytes / Q000201:enzymology*; D046911:Macromolecular Substances; D010196:Pancreatic Elastase / Q000032:analysis* / Q000276:immunology; D012661:Semen / Q000201:enzymology; D000515:alpha 1-Antitrypsin / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D046911:Macromolecular Substances; D000515:alpha 1-Antitrypsin; D010196:Pancreatic Elastase