Occurrence of neutral endopeptidase activity in the cat carotid body and its significance in chemoreception.
The carotid body contains both tachykinins and enkephalins. Neutral endopeptidase (NEP, E.C. 3.4.24.11), has been suggested to involve in the metabolism of these neuropeptides in several organs. In the present study we determined neutral endopeptidase activity of the cat carotid body and assessed its significance in chemoreception. The cytosolic and membrane fractions of the carotid body contained NEP-like activity whereas it occurred only in the membrane fractions of the superior cervical and the nodose ganglia. Phosphoramidon, thiorphan and metal ion chelators inhibited NEP-like activity of all the 3 tissues studied; other protease inhibitors, however, were ineffective. Close carotid body administration of phosphoramidon significantly potentiated the carotid body response to low PO2 but not to hypercapnia. The enhanced response to hypoxia following phosphoramidon was further augmented by naloxone, an enkephalin antagonist. These results demonstrate that the glomus tissue contains detectable amounts of NEP-like activity and its inhibition selectively affects the hypoxic response of the carotid body.
Kumar
G K
GK
Department of Medicine, Case Western Reserve University, Cleveland, OH 44106.
Runold
M
M
Ghai
R D
RD
Cherniack
N S
NS
Prabhakar
N R
NR
D000818:Animals; D002344:Carotid Body / Q000201:enzymology* / Q000502:physiology; D002415:Cats; D002628:Chemoreceptor Cells / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006020:Glycopeptides / Q000494:pharmacology*; D015260:Neprilysin / Q000378:metabolism*
D004791:Enzyme Inhibitors; D006020:Glycopeptides; D015260:Neprilysin; C008890:phosphoramidon