Determination of amoxicillin in plasma by high-performance liquid chromatography with fluorescence detection after online oxidation.
A simplified high-performance liquid chromatographic method for the determination of amoxicillin in plasma is described. Specific and sensitive fluorescence detection was achieved by on-line post-column electrochemical oxidation, using an electrochemical detector. Owing to the high specificity of the detection system, deproteinized plasma samples could be injected directly without prior treatment. This method permits a very fast and reproducible determination of amoxicillin in plasma on a routine basis at levels, down to 50 ng/ml. The absolute detection limit is about 10 pg injected.
Mascher
H
H
Pharm-Analyt Lab. GmbH, Traiskirchen, Vienna, Austria.
Kikuta
C
C
D000658:Amoxicillin / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D010084:Oxidation-Reduction
D000658:Amoxicillin