Crystallization and preliminary x-ray diffraction study of neurotoxin-I from Naja naja oxiana venom.
Crystals of the neurotoxin-I (NTX-I) from the venom of the middle Asian cobra Naja naja oxiana have been grown by vapour diffusion and dialysis methods. The crystals belong to space group P2(1)2(1)2 with dimension of a = 25.19 A, b = 75.59 A, c = 36.09 A and diffract to 1.9 A resolution. The asymmetric unit contains one molecule (Vm = 2.2 A/Da). Using the molecule of alpha-cobratoxin (CTX) as a starting model for NTX-I structure determination coordinates of C alpha atoms of the NTX-I molecule were obtained and the position of NTX-I in the unit cell was derived.
Mikhailov
A M
AM
Institute of Crystallography of the USSR Academy of Sciences, Moscow.
Nickitenko
A V
AV
Trakhanov
S D
SD
Vainshtein
B K
BK
Chetverina
E V
EV
D000595:Amino Acid Sequence; D000818:Animals; D003039:Cobra Neurotoxin Proteins; D003461:Crystallography; D004546:Elapid Venoms; D008969:Molecular Sequence Data; D011487:Protein Conformation; D014961:X-Ray Diffraction
D003039:Cobra Neurotoxin Proteins; D004546:Elapid Venoms