[G-banding and high resolution chromosome study of a human lung adenocarcinoma cell line LGC-7910].
G-banding and high resolution chromosome banding techniques were used in studying a human lung adenocarcinoma cell line LGC-7910. One hundred cells were counted at Passage 150. Ten, twelve and ten cells were analyzed at Passages 150, 173 and 183, respectively. The model number of chromosome in this cell line was 67-69. A number of marker chromosomes related to complex chromosomal rearrangement was observed. The analysis of chromosomes at the different passages showed a stability during subculture of the cell line. It was found that the cell line had partial deletion of the short arm on chromosome 7 (with breakpoint at 7p15.3-p13), rearrangement of the arm on chromosome 1 (with breakpoint at 1p13.1) and increase in chromosome 7 number. The results suggested that such chromosome alteration be related to the expression of activated oncogenes during carcinogenesis.
Guo
L R
LR
Haerbin Medical College.
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D002871:Chromosome Banding; D002872:Chromosome Deletion; D002897:Chromosomes, Human, Pair 7; D005819:Genetic Markers; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D014407:Tumor Cells, Cultured
D005819:Genetic Markers