title: Iron acquisition in Pasteurella haemolytica: expression and identification of a bovine-specific transferrin receptor.
abstract: Seven type 1 field isolates of Pasteurella haemolytica were screened for their ability to use different transferrins as a source of iron for growth. All seven strains were capable of using bovine but not human, porcine, avian, or equine transferrin. A screening assay failed to detect siderophore production in any of the strains tested. Iron-deficient cells from these strains expressed a binding activity, specific for bovine transferrin, that was regulated by the level of iron in the medium. Inhibition of expression by translation and transcription inhibitors suggested that iron regulation was occurring at the gene level. Affinity isolation of receptor proteins from all seven strains with biotinylated bovine transferrin identified a 100-kilodalton iron-regulated outer membrane protein as the bovine transferrin receptor. Iron-regulated outer membrane proteins of 71 and 77 kilodaltons were isolated along with the 100-kilodalton protein when less stringent washing procedures were employed in the affinity isolation procedure.
authors.affiliation: Department of Microbiology and Infectious Diseases, University of Calgary, Alberta, Canada.
authors.forename: J A
authors.initials: JA
authors.lastname: Ogunnariwo
authors.forename: A B
authors.initials: AB
authors.lastname: Schryvers
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000302:isolation & purification; D002417:Cattle; D002455:Cell Division; D002846:Chromatography, Affinity; D015964:Gene Expression Regulation, Bacterial; D007501:Iron / Q000378:metabolism*; D010325:Pasteurella / Q000378:metabolism*; D011990:Receptors, Transferrin / Q000096:biosynthesis* / Q000302:isolation & purification; D013045:Species Specificity; D014158:Transcription, Genetic; D014168:Transferrin / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D011990:Receptors, Transferrin; D014168:Transferrin; D007501:Iron