title: Changes in lipid composition and desaturase activities of duodenal mucosa induced by dietary fat.
abstract: In the present work we have studied the effects of feeding either olive or sunflower oil on lipid composition and desaturase activities of duodenal mucosa microsomes. Duodenal microsomes prepared from dogs fed the sunflower oil diet showed higher percentages of saturated, of linoleic and of n - 3 polyunsaturated fatty acids as well as lower levels of oleic, dihomo-gamma-linolenic and arachidonic acids in phosphatidylcholine and phosphatidylethanolamine than those prepared from animals fed the olive oil diet. In sphingomyelin, the dietary supplementation did not produce significant differences between the two groups. The cholesterol/phospholipid molar ratio was higher in the sunflower oil group than in the olive oil group. The in vitro delta 9-desaturase activity was higher in microsomes from the olive oil dogs. The delta 6-desaturase activity was similar in microsomes from the two groups and lower than that found for delta 9-desaturase activity. Desaturase activities were higher in duodenal microsomes than those previously found for liver microsomes.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, University of Granada.
authors.forename: M D
authors.initials: MD
authors.lastname: Girón
authors.forename: F J
authors.initials: FJ
authors.lastname: Mataix
authors.forename: M D
authors.initials: MD
authors.lastname: Suárez
mesh_terms: D000818:Animals; D004041:Dietary Fats / Q000494:pharmacology*; D004285:Dogs; D004386:Duodenum / Q000378:metabolism; D044943:Fatty Acid Desaturases / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism; D050356:Lipid Metabolism; D008297:Male; D000069463:Olive Oil; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D010743:Phospholipids / Q000096:biosynthesis / Q000302:isolation & purification; D010938:Plant Oils / Q000494:pharmacology; D000074242:Sunflower Oil
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids; D008041:Linoleic Acids; D000069463:Olive Oil; D010169:Palmitic Acids; D010743:Phospholipids; D010938:Plant Oils; D000074242:Sunflower Oil; D019308:Palmitic Acid; D019787:Linoleic Acid; D044943:Fatty Acid Desaturases