title: Experimental glycerol myopathy: a histological study.
abstract: Histopathological changes induced by the intramuscular injection of glycerol were studied in the muscle fibers of rabbits. Fifteen minutes after the injection of 1 ml of 50% (v/v) glycerol, hypercontraction of fibers, disruption of the plasma membrane, and invasion of lanthanum into the sarcoplasm were observed. Between 12 and 24 h after the injection, more extensive pathological changes were seen which included: variation in fiber size; degeneration and necrosis of muscle fibers; hypercontraction of fibers by light microscopy; disruption of the plasma membrane by electron microscopy; vacuolar changes; hypercontraction of myofibrils, and selective loss of Z-bands. Between 7 and 14 days after glycerol injection, extensive regenerative changes were seen. The degenerative changes were similar to those seen in muscle in Duchenne muscular dystrophy, suggesting that a similar mechanism may be involved in the two conditions, so that experimental glycerol myopathy could be a good model for pathophysiological studies on Duchenne muscular dystrophy.
authors.affiliation: First Department of Internal Medicine, School of Medicine, University of Tokushima, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kawai
authors.forename: H
authors.initials: H
authors.lastname: Nishino
authors.forename: K
authors.initials: K
authors.lastname: Kusaka
authors.forename: T
authors.initials: T
authors.lastname: Naruo
authors.forename: Y
authors.initials: Y
authors.lastname: Tamaki
authors.forename: M
authors.initials: M
authors.lastname: Iwasa
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D002118:Calcium / Q000032:analysis; D005260:Female; D005990:Glycerol / Q000633:toxicity*; D008854:Microscopy, Electron; D009132:Muscles / Q000187:drug effects / Q000473:pathology* / Q000648:ultrastructure; D009135:Muscular Diseases / Q000139:chemically induced / Q000473:pathology* / Q000503:physiopathology; D009336:Necrosis; D011817:Rabbits; D012038:Regeneration
chemical_list: D000135:Acid Phosphatase; D005990:Glycerol; D002118:Calcium