Distortions induced in double-stranded oligonucleotides by the binding of cis- or trans-diammine-dichloroplatinum(II) to the d(GTG) sequence.
Conformational changes induced in double-stranded oligonucleotides by the binding of trans- or cis-diamminedichloro platinum(II) to the d(GTG) sequence have been characterized by means of melting temperatures, electrophoretic migrations in non-denaturing polyacrylamide gels, reactivities with the artificial nuclease Phenanthroline-copper and with chemical probes. The cis-platinum adduct behaves more as a centre of directed bend than as a hinge joint, the induced bend angle being of the order of 25-30 degrees. The double helix is locally denatured over 2 base pairs (corresponding to the platinated 5'G residue and the central T residue) and is distorted over 4-5 base pairs. The trans-platinum adduct behaves also more as a centre of directed bend than as a hinge joint, the induced bend angle being of the order of 60 degrees. The double helix is locally denatured over 4 base pairs (corresponding to the immediately 5'T residue adjacent to the adduct and to the three base residues of the adduct). Both the cis- and trans-platinum adducts decrease the thermal stability of the double helix.
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Centre de Biophysique Moléculaire, CNRS, Orléans, France.
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D000079:Acetaldehyde / Q000031:analogs & derivatives; D001483:Base Sequence; D001665:Binding Sites; D002945:Cisplatin / Q000378:metabolism*; D003300:Copper; D004047:Diethyl Pyrocarbonate; D015180:Electrophoresis, Gel, Two-Dimensional; D007536:Isomerism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides; D009993:Osmium Tetroxide; D010618:Phenanthrolines; D012966:Sodium Cyanide; D013696:Temperature
D009838:Oligodeoxyribonucleotides; D010618:Phenanthrolines; C034697:transplatin; D003300:Copper; C004656:chloroacetaldehyde; D000079:Acetaldehyde; D004047:Diethyl Pyrocarbonate; D012966:Sodium Cyanide; D009993:Osmium Tetroxide; D002945:Cisplatin