title: Genetic and fine structure analysis of unc-26(IV) and adjacent regions in Caenorhabditis elegans.
abstract: The genetic organization of unc-26(IV) and adjacent regions was studied in Caenorhabditis elegans. We constructed a fine structure genetic map of unc-26(IV), a gene that affects locomotion and pharyngeal muscle movement but not muscle structure. Eleven alleles were positioned relative to each other recombinationally and were classified according to phenotypic severity. The unc-26 gene spans at least 0.026 map units, which is exceptionally large for a C. elegans gene. All but one allele, e205, are amorphic alleles. Interestingly, e205 is hypomorphic but also suppressible by the amber suppressor sup-7. Nineteen lethal mutations in the unc-26 region were isolated and characterized. The unc-26 region is subdivided into four zones by five deficiency breakpoints. These mutations fall into 15 complementation groups. The stages of development affected by these mutations were determined.
authors.affiliation: Department of Biological Sciences, Simon Fraser University, Burnaby, B.C., Canada.
authors.forename: D L
authors.initials: DL
authors.lastname: Charest
authors.forename: D V
authors.initials: DV
authors.lastname: Clark
authors.forename: M E
authors.initials: ME
authors.lastname: Green
authors.forename: D L
authors.initials: DL
authors.lastname: Baillie
mesh_terms: D000483:Alleles; D000818:Animals; D002107:Caenorhabditis / Q000235:genetics*; D002874:Chromosome Mapping; D005796:Genes; D005804:Genes, Lethal; D005816:Genetic Complementation Test; D008124:Locomotion; D009154:Mutation; D010641:Phenotype; D011995:Recombination, Genetic