[Use of fluorescent carbocyanine dyes in the study of the organization of the pathways in autopsy material of the human brain].
Our report provides evidence that fluorescent carbocyanine dyes (diI and diO) can be used in experimental anatomical studies of the fixed autopsy human brain. The dyes transported in both anterograde and retrograde directions, providing labeling of axons with collaterals and neurons including dendrites. To study the retrograde labeling of pyramidal neurons and anterogradely labeling of afferent fibers in human motor cortex, we applied diI and diO to the white matter, I and III layers of cortex. During 2 months there was no evidence of passive diffusion from labeled fibers and neurons to other neurons or glia. This method will be useful for identifying alterations of neuronal connections associated with neurological and psychiatric disorders.
Belichenko
P V
PV
D000368:Aged; D001344:Autopsy; D001369:Axons / Q000473:pathology; D001921:Brain / Q000473:pathology*; D002232:Carbocyanines; D003712:Dendrites / Q000473:pathology; D005456:Fluorescent Dyes; D006801:Humans; D008297:Male; D009044:Motor Cortex / Q000473:pathology; D009474:Neurons / Q000473:pathology; D011804:Quinolines
D002232:Carbocyanines; D005456:Fluorescent Dyes; D011804:Quinolines
Ispol'zovanie fliuorestsentnykh karbotsianinovyk krasiteleĭ dlia izucheniia organizatsii provodiashchikh puteĭ autopsiĭnogo materiala mozga cheloveka.