title: Detection of auto-anti-idiotypic antibodies to Lol p I (rye I) IgE antibodies in human sera by the use of murine idiotypes: levels in atopic and non-atopic subjects and effects of immunotherapy.
abstract: Anti-idiotypic antibodies (anti-Id Abs) are involved in the regulation of a number of immune responses including the IgE antibody production. In atopic patients, the increased synthesis of IgE antibodies could be related to a defective production of regulatory anti-Id Abs. In the present study, we first developed a sensitive assay for measuring the levels of anti-Id Abs directed against antibodies specific for Lol p I, the major allergenic determinant of Lolium perenne (rye grass). In this assay, we used previously described murine monoclonal anti-Lol p I antibodies that were shown to share epitopic specificities with human anti-Lol p I IgE and IgG antibodies, thus short-cutting the need for purification of F(ab')2 fragments of human IgG Abs and insuring optimal specificity and sensitivity. Levels of anti-Id Abs against two anti-Lol p I monoclonal antibodies (290A-167, 348A-6) were higher in normal volunteers than in untreated atopic patients. Specific immunotherapy increased the levels of anti-Id Abs to those of normal volunteers. These observations suggest a role for the Id-anti-Id network in the regulation of IgE antibody production.
authors.affiliation: Unité de Recherche Inflammation et Immunologie-Rhumatologie Centre Hospitalier de l'Université Laval, Québec City, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Hébert
authors.forename: D
authors.initials: D
authors.lastname: Bernier
authors.forename: W
authors.initials: W
authors.lastname: Mourad
mesh_terms: D000485:Allergens / Q000627:therapeutic use; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity / Q000276:immunology; D052179:Antigens, Plant; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis / Q000276:immunology*; D007074:Immunoglobulin G / Q000032:analysis; D010940:Plant Proteins; D011058:Pollen / Q000276:immunology*; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology* / Q000628:therapy
chemical_list: D000485:Allergens; D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D052179:Antigens, Plant; D007074:Immunoglobulin G; C042814:Lol p I protein, Lolium perenne; D010940:Plant Proteins; D007073:Immunoglobulin E