TGF alpha-anti-Tac(Fv)-PE40: a bifunctional toxin cytotoxic for cells with EGF or IL2 receptors.
Conventional immunotoxins and chimeric toxins made in bacteria are directed to only one receptor or antigen on target cells. In this report we describe the construction of a chimeric molecule TGF alpha-anti Tac(Fv)-PE40 which is composed of human transforming growth factor type alpha attached to anti-Tac(Fv) which is in turn attached to PE40, a form of pseudomonas exotoxin, devoid of its cell recognition domain. TGF alpha-anti-Tac(Fv)-PE40 is a bifunctional toxin that is produced in E. coli and is active on cells bearing either IL2 or EGF receptors.
Batra
J K
JK
Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
Chaudhary
V K
VK
FitzGerald
D
D
Pastan
I
I
D036002:ADP Ribose Transferases; D000595:Amino Acid Sequence; D001427:Bacterial Toxins; D001483:Base Sequence; D002460:Cell Line; D066246:ErbB Receptors / Q000378:metabolism*; D005098:Exotoxins / Q000008:administration & dosage* / Q000235:genetics; D005822:Genetic Vectors; D006801:Humans; D000922:Immunotoxins / Q000235:genetics*; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D015375:Receptors, Interleukin-2 / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000633:toxicity*; D015291:Transforming Growth Factors / Q000008:administration & dosage / Q000235:genetics*; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D066246:ErbB Receptors; D005098:Exotoxins; D000922:Immunotoxins; D015375:Receptors, Interleukin-2; D011993:Recombinant Fusion Proteins; D015291:Transforming Growth Factors; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A