title: Demonstrating immune-related antigens in Alzheimer's disease brain tissue.
abstract: Immune system associated markers have recently been reported in Alzheimer's disease (AD) brain. However, previous studies have not consistently revealed these antigens in the central nervous system. Immunohistochemistry of HLA-DR and other immune antigens (e.g., CD4+, IL-2R) in AD cortical tissue was compared using paraformaldehyde-fixed versus snap-frozen unfixed tissue. In addition, a free-floating immunohistochemistry method was contrasted with an on slide procedure. Even with an HLA-DR antibody known to work well on fixed tissue, unfixed tissue gave superior results. Free-floating immunohistochemistry also increased sensitivity. Where morphologic considerations are paramount, brief fixation with free-floating methods provides a reasonable alternative.
authors.affiliation: L. J. Roberts Center, Institute for Biogerontology Research, Sun City, AZ 85351.
authors.forename: J
authors.initials: J
authors.lastname: Rogers
authors.forename: E J
authors.initials: EJ
authors.lastname: Mufson
mesh_terms: D000544:Alzheimer Disease / Q000276:immunology* / Q000473:pathology; D001921:Brain / Q000276:immunology* / Q000473:pathology; D006684:HLA-DR Antigens / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods
chemical_list: D006684:HLA-DR Antigens