title: Microdissection of the fragile X region.
abstract: We have microdissected and cloned the region around the fragile site at Xq27.3 on the human X chromosome. All of the clones tested map to the Xq27-Xq28 region, and detailed mapping on a panel of somatic cell hybrids indicates that the microdissected library contains sequences derived from both sides of the fragile X mutation. Some of these clones give signals in rodent DNA. This library demonstrates the power of microdissection for the identification of potential coding sequences near a disease locus and provides a promising resource for the identification of the fragile X mutation.
authors.affiliation: Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, England.
authors.forename: R N
authors.initials: RN
authors.lastname: MacKinnon
authors.forename: M C
authors.initials: MC
authors.lastname: Hirst
authors.forename: M V
authors.initials: MV
authors.lastname: Bell
authors.forename: J E
authors.initials: JE
authors.lastname: Watson
authors.forename: U
authors.initials: U
authors.lastname: Claussen
authors.forename: H J
authors.initials: HJ
authors.lastname: Ludecke
authors.forename: G
authors.initials: G
authors.lastname: Senger
authors.forename: B
authors.initials: B
authors.lastname: Horsthemke
authors.forename: K E
authors.initials: KE
authors.lastname: Davies
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D002871:Chromosome Banding; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D015342:DNA Probes; D005600:Fragile X Syndrome / Q000235:genetics*; D005784:Gene Amplification; D006801:Humans; D006822:Hybrid Cells; D007621:Karyotyping; D015183:Restriction Mapping; D012729:Sex Chromosome Aberrations / Q000235:genetics*; D014960:X Chromosome
chemical_list: D015342:DNA Probes