The development of identified neurosecretory cells in the tobacco hornworm, Manduca sexta.
The prothoracicotropic hormone (PTTH) is a principal neuropeptide regulator of insect postembryonic molting and metamorphosis. In the tobacco hornworm, Manduca sexta, PTTH is produced by two neurosecretory cells (NSC) located in each protocerebral lobe of the brain. The development of these neurons, the L-NSC III, has been investigated immunocytologically to establish the time course of their morphological differentiation. PTTH may be one of the earliest neuropeptides expressed in insect embryos. PTTH-immunoreactivity was initially detected in the somata at 24 to 30% of embryonic development. Neurites sprouted shortly thereafter and began to grow medially through the brain anlage. By 42% embryonic development, the neurites had decussated to the contralateral brain lobe. As development progressed, the L-NSC III neurites grew along specific tracts through the contralateral brain lobe reaching the ventrolateral regions of the brain by approximately 60% development. The axons exited the brain through a retrocerebral nerve, the nervi corporis cardiaci I + II. At approximately 63% development, the axons innervated the corpus allatum and began branching to form neurohemal terminals for PTTH release. At 60% development, short collaterals began extending in the protocerebral neuropil. During the remainder of embryogenesis, both the dendritic collaterals and the terminal neurohemal varicosities continued to elongate and arborize. By 85% embryonic development, the basic architecture of the L-NSC III was established.
Westbrook
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Department of Biology, University of North Carolina, Chapel Hill 27599-3280.
Bollenbacher
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D000818:Animals; D012831:Bombyx / Q000032:analysis*; D001921:Brain / Q000166:cytology / Q000196:embryology; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000502:physiology*; D007814:Larva; D007915:Lepidoptera / Q000196:embryology*; D009036:Moths / Q000196:embryology*; D009479:Neuropeptides / Q000032:analysis*; D009490:Neurosecretory Systems / Q000166:cytology / Q000196:embryology
D009479:Neuropeptides