Lymphocyte-mediated inhibition of platelet cytotoxic functions during Hymenoptera venom desensitization: characterization of a suppressive lymphokine.
Recently, it has been shown that platelets, through a receptor for the Fc fragment of IgE, could be specially triggered by venom allergens in hypersensitivity to hymenoptera, generating cytocidal mediators toward Schistosoma mansoni larvae, and oxygen metabolites measured by chemiluminescence. After rush immunotherapy, a depressed platelet response was demonstrated to be associated with the production of lymphokine(s). Here we report the characterization of a factor present in supernatants of antigen-stimulated T cells from patients after hymenoptera venom desensitization which is able to inhibit platelet cytotoxic functions in a dose-dependent manner. The optimal inhibition was observed with supernatants obtained after T lymphocyte stimulated with 10(-5) micrograms venom allergen/ml. Once specifically produced the platelet-suppressive effect of lymphocyte supernatants was not antigen specific. The producing T cell subpopulation was identified as CD8+. This lymphokine had an approximate molecular mass of 25 kDa and a pI of 4.8. It was heat and acid stable and sensitive to trypsin and proteinase K but not to neuraminidase. This platelet inhibitory activity was absorbed by platelet membrane suggesting its binding to a receptor. These properties were very similar to a previously described platelet activity suppressive lymphokine, suggesting the participation of this lymphokine in the mechanisms of rush desensitization.
Tsicopoulos
A
A
Centre d'Immunologie et de Biologie Parasitaire, INSERM 167-CNRS 624, Institut Pasteur, Lille, France.
Tonnel
A B
AB
Vorng
H
H
Joseph
M
M
Wallaert
B
B
Kusnierz
J P
JP
Pestel
J
J
Capron
A
A
D000818:Animals; D001792:Blood Platelets / Q000276:immunology*; D003470:Culture Media; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D003888:Desensitization, Immunologic; D006801:Humans; D006927:Hymenoptera / Q000276:immunology*; D066298:In Vitro Techniques; D015539:Platelet Activation / Q000276:immunology; D012550:Schistosoma mansoni / Q000276:immunology; D013491:Suppressor Factors, Immunologic / Q000302:isolation & purification*; D013601:T-Lymphocytes / Q000276:immunology
D003470:Culture Media; D013491:Suppressor Factors, Immunologic