title: Electron microscopic observation of calcitonin gene-related peptide-like immunoreactivity in the organ of Corti of the rat.
abstract: Calcitonin gene-related peptide (CGRP)-like immunoreactive (CGRP-IR) nerve terminals in the organ of Corti of rats were studied by light and electron microscopy. Surface preparation of the organ of Corti were immunostained using anti-CGRP antiserum for avidin-biotin immunohistochemistry. Dense CGRP-IR fiber bundles were observed by light microscopy in the inner spiral bundles, tunnel spiral bundles and outer spiral bundles. Electron microscopic analysis indicated that CGRP-IR fibers belong to efferent nerves. In the inner spiral bundles, the CGRP-IR fibers showed a direct contact mainly with non-immunoreactive afferent fibers. Some CGRP-IR nerve endings in the inner spiral bundles formed contacts directly with inner hair cells. In the outer spiral bundles, CGRP-IR fibers formed synaptic contacts exclusively with the outer hair cells. It should be noted that the number of synapses of CGRP nerve endings with outer hair cells varied depending upon the sub-row: a falling gradient in number occurred along the inner-outer axis. Our results suggest that CGRP acts as an efferent neuromodulator in the organ of Corti.
authors.affiliation: Department of Neuroanatomy, Osaka University Medical School, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kuriyama
authors.forename: S
authors.initials: S
authors.lastname: Shiosaka
authors.forename: M
authors.initials: M
authors.lastname: Sekitani
authors.forename: Y
authors.initials: Y
authors.lastname: Tohyama
authors.forename: M
authors.initials: M
authors.lastname: Kitajiri
authors.forename: T
authors.initials: T
authors.lastname: Yamashita
authors.forename: T
authors.initials: T
authors.lastname: Kumazawa
authors.forename: M
authors.initials: M
authors.lastname: Tohyama
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D008854:Microscopy, Electron; D009925:Organ of Corti / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D015740:Calcitonin Gene-Related Peptide