[The structuro-functional heterogeneity of an artificial oxygen carrier based on hemoglobin].
Pyridoxylated polymerized hemoglobin (PP-Hb) was fractionated by the method of high pressure liquid chromatography, Molecular weight distribution, oxygen carrying capacity and antigenic characteristics of separated fractions have been investigated. It was demonstrated that a fraction may be separated from the crude PP-Hb, which is greatly homogeneous if structural and functional characteristics are concerned, and also optimal according to the parameters investigated. So, by a corresponding fractionation method it is possible to overcome the main limitation for use of PP-Hb as an artificial oxygen carrier--its heterogeneity.
Viazova
E P
EP
Azhigirova
M A
MA
Fetisova
L V
LV
Shuvalova
A L
AL
Zeĭnalov
A I
AI
D001802:Blood Substitutes / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D006454:Hemoglobins / Q000032:analysis* / Q000302:isolation & purification; D006801:Humans; D007120:Immunochemistry; D007122:Immunoelectrophoresis; D066298:In Vitro Techniques; D008422:Materials Testing; D008970:Molecular Weight; D010100:Oxygen / Q000097:blood*; D011732:Pyridoxal Phosphate / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D013329:Structure-Activity Relationship
D001802:Blood Substitutes; D006454:Hemoglobins; C030939:polyhemoglobin-pyridoxal-5-phosphate; D011732:Pyridoxal Phosphate; D010100:Oxygen
Strukturno-funktsional'naia geterogennost' iskusstvennogo perenoschika kisloroda na osnove gemoglobina.