Transfection by DNA-nuclear protein HMG1 complexes: raising of efficiency and role of DNA topology.
We have developed a novel and efficient transfection method based on the introduction of foreign DNA into mammalian cells in form of complexes of vector DNA with the nuclear protein HMG1. In this study, it is shown that a stabilization of the complexes against dilution dissociation by addition of soluble CaCl2 or by excessive HMG1 enhances the transfection efficiency. Furthermore, there are no differences in the transfection abilities between the 3 topological DNA forms, viz., supercoiled, open relaxed and linear DNA, if delivered to cells as HMG1-DNA complexes. It is further shown that transfection-inactive complexes of the core histones with foreign DNA can be activated in transfection by the addition of HMG1.
Böttger
M
M
Central Institute of Molecular Biology, Academy of Sciences, G.D.R., Berlin-Buch.
Platzer
M
M
Kiessling
U
U
Strauss
M
M
D000818:Animals; D004247:DNA; D004278:DNA, Superhelical; D066298:In Vitro Techniques; D007739:L Cells; D051379:Mice; D015394:Molecular Structure; D009687:Nuclear Proteins; D010957:Plasmids; D014162:Transfection
D004278:DNA, Superhelical; D009687:Nuclear Proteins; D004247:DNA