title: Localization of pyruvate carboxylase in organic acid-producing Aspergillus strains.
abstract: The localization of pyruvate carboxylase (cytosolic or mitochondrial) was studied in nine different Aspergillus species (14 strains). In some species (A. aculeatus, A. flavus, A. foetidus, A. nidulans, A. ochraceus, and A. sojae), the pyruvate carboxylase activity could be detected only in the cytosolic fraction of the cells. Pyruvate carboxylase has been found only in the mitochondrial fraction of two strains of Aspergillus wentii. In Aspergillus oryzae and in five strains of Aspergillus niger, pyruvate carboxylase activity was detected both in the mitochondrial fraction and in the cytosol. There was no quantitative or qualitative correlation between the activities of pyruvate carboxylase in the mitochondrial and cytosolic fractions of the cells and the ability of the various Aspergillus strains to accumulate different organic acids.
authors.affiliation: Department of Applied Microbiology, Hebrew University, Jerusalem, Israel.
authors.forename: A
authors.initials: A
authors.lastname: Bercovitz
authors.forename: Y
authors.initials: Y
authors.lastname: Peleg
authors.forename: E
authors.initials: E
authors.lastname: Battat
authors.forename: J S
authors.initials: JS
authors.lastname: Rokem
authors.forename: I
authors.initials: I
authors.lastname: Goldberg
mesh_terms: D001230:Aspergillus / Q000201:enzymology*; D002950:Citrate (si)-Synthase / Q000378:metabolism; D002951:Citrates / Q000378:metabolism*; D019343:Citric Acid; D003600:Cytosol / Q000201:enzymology; D005942:Gluconates / Q000378:metabolism*; D008291:Malate Dehydrogenase / Q000378:metabolism; D008293:Malates / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology; D011766:Pyruvate Carboxylase / Q000378:metabolism*
chemical_list: D002951:Citrates; D005942:Gluconates; D008293:Malates; D019343:Citric Acid; C030298:malic acid; D008291:Malate Dehydrogenase; D002950:Citrate (si)-Synthase; D011766:Pyruvate Carboxylase; C030691:gluconic acid