Electron-impact ionization detection of scopolamine by gas chromatography-mass spectrometry in rat plasma and brain.
Scopolamine, a muscarinic receptor antagonist, was measured in rat plasma and brain using gas chromatography-mass spectrometry with electron-impact ionization detection. Extracted scopolamine was either directly derivatized or first hydrolyzed to scopine, then derivatized with heptafluorobutyric anhydride, separated on a capillary column and quantified by mass fragmentography. Electron-impact ionization produced a common fragment peak at m/z 138 that was monitored along with trideuterated scopolamine, an internal standard (m/z 141). The method can be used to measure scopolamine concentrations of 2 ng/ml in rat plasma and 20 ng/g in rat brain.
Deutsch
J
J
Laboratory of Neurosciences, National Institute on Aging, National Institutes of Health, Bethesda, MD 20892.
Soncrant
T T
TT
Greig
N H
NH
Rapoport
S I
SI
D000818:Animals; D001923:Brain Chemistry; D008401:Gas Chromatography-Mass Spectrometry; D051381:Rats; D012601:Scopolamine / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics
D012601:Scopolamine