Improved fragile site detection with trimethoprim.
The effect of trimethoprim, an inhibitor of dihydrofolate reductase, in demonstrating the presence of fragile sites on human chromosomes was investigated. Lymphocyte cultures with 20 mg/l trimethoprim added at the onset of incubation gave consistently higher frequencies of fragile sites than the other culture regimes tested.
McLean
D A
DA
Department of Pathology, University of Dundee, Ninewells Hospital, UK.
Faed
M J
MJ
D043283:Chromosome Fragile Sites; D002873:Chromosome Fragility; D005260:Female; D005600:Fragile X Syndrome / Q000175:diagnosis*; D006801:Humans; D008297:Male; D012729:Sex Chromosome Aberrations / Q000175:diagnosis*; D012737:Sex Factors; D014295:Trimethoprim
D014295:Trimethoprim