[Development of passive hemagglutination assay of measles and utilization in serological diagnosis].
The passive hemagglutination assay (PHA) was developed. Preparation of antigen and its stability, sensitivity and specificity was studied. The result showed that the yield of ultrasonic treated antigen is high and its titer has no change in 4 degrees C storage at least 1 month. The sensitized sheep erythrocyte with this antigen have a high specificity and sensitivity. The lysophilized blood cells could stored for a long time in 4 degrees C. A comparison of PHA with hemagglutination inhibition test was carried out by examination of 354 samples of sera from healthy children. The GMT of titer of antibodies of measles were 26.20 and 13.92 respectively. The result showed that the two methods were highly related.
Zhang
R
R
Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing.
D000956:Antigens, Viral / Q000302:isolation & purification; D006386:Hemagglutination Tests / Q000379:methods; D006801:Humans; D008457:Measles / Q000175:diagnosis*; D008459:Measles virus / Q000276:immunology
D000956:Antigens, Viral