Effect of immunotherapy on the production of IgE regulatory soluble factors. The response of normal lymphocytes.
IgE synthesis is controlled not only by antigen-specific mechanism but also by other factors which selectively affect the IgE isotype. Several groups demonstrated that factors released by lymphocytes were involved in this regulation. Our previous investigations were also directed in this field. Immunotherapy improves clinical symptoms in allergic respiratory diseases and produces cellular and humoral changes that affect IgE production. So we decided to investigate if immunotherapy had any influence in the production of soluble factors. Bidirectional mixed cultures were performed with lymphocytes from healthy controls and allergic patients with different time periods of IT, and the supernatants obtained from chromatography were tested to determine their effect on IgE synthesis of normal lymphocytes. IT time periods exerted influence on the production of enhancing factors. Suppressor factors derived from allergic patients had no effect on IgE synthesis of normal lymphocytes.
Tokushima
M
M
Departamento de Alergología, Facultad de Medicina, Universidad de Navarra, Pamplona, Spain.
Sanz
M L
ML
de las Marinas
M D
MD
Fernández
J F
JF
Oehling
A
A
D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D003888:Desensitization, Immunologic; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis* / Q000494:pharmacology; D008222:Lymphokines / Q000096:biosynthesis* / Q000494:pharmacology; D029584:Prostatic Secretory Proteins; D012130:Respiratory Hypersensitivity / Q000276:immunology / Q000473:pathology / Q000628:therapy; D013491:Suppressor Factors, Immunologic / Q000096:biosynthesis* / Q000494:pharmacology; D013601:T-Lymphocytes / Q000378:metabolism*
C037161:IGE class-specific suppressor factor; D008222:Lymphokines; D029584:Prostatic Secretory Proteins; D013491:Suppressor Factors, Immunologic; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors; D007073:Immunoglobulin E