title: Time-variations of pretreatment peripheral blood S + G2/M-phase size determined by flow cytometry in adult acute myeloid leukaemia.
abstract: The circadian and seasonal variations of pretreatment proliferative activity of peripheral blood (PB) as PB S + G2/M-phase size was determined by flow cytometry in 61 adult patients with acute myeloid leukaemia (AML). Pretreatment PB S-phase (p less than 0.002), G2 + M-phase (p less than 0.008) and S + G2/M-phase size are statistically correlated to the time of sampling, with the highest phase size at the end of the day. Time-variations of the blast cell count are slightly significant (p = 0.049). Cytological diagnosis-related differences in S + G2/M-phase (p less than 0.003) and white blood cell count (p less than 0.04) time-variations are observed. For all patients, no seasonal variations can be drawn, but in AML 1 (p less than 0.029) and AML 4-5 patients (p less than 0.003), the circadian variations of S + G2/M are affected by the seasons. The present results suggest that time may be taken into account in the monitoring of chemotherapy in acute leukaemia.
authors.affiliation: Service de médecine A, CHU de Brabois, Nancy, France.
authors.forename: A
authors.initials: A
authors.lastname: Guerci
authors.forename: P
authors.initials: P
authors.lastname: Scheid
authors.forename: P
authors.initials: P
authors.lastname: Feugier
authors.forename: J
authors.initials: J
authors.lastname: Pierrez
authors.forename: N
authors.initials: N
authors.lastname: Frenkiel
authors.forename: O
authors.initials: O
authors.lastname: Guerci
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002940:Circadian Rhythm; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007399:Interphase; D015470:Leukemia, Myeloid, Acute / Q000097:blood*; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D008938:Mitosis; D010507:Periodicity; D012621:Seasons