Intranasal immunization of mice with recombinant protein antigen of serotype c Streptococcus mutans and cholera toxin B subunit.
The cholera toxin subunit and the recombinant cell-surface antigen (molecular mass of 190,000 Da) were administered intranasally to BALB/c mice. After 30 days, the mice were immunized intranasally with the recombinant protein antigen alone. High serum IgG and salivary IgA responses to the protein antigen were induced by the intranasal immunization.
Takahashi
I
I
Department of Dental Research, National Institute of Health, Tokyo, Japan.
Okahashi
N
N
Kanamoto
T
T
Asakawa
H
H
Koga
T
T
D000281:Administration, Intranasal; D000818:Animals; D000942:Antigens, Bacterial / Q000008:administration & dosage*; D002772:Cholera Toxin / Q000008:administration & dosage*; D005260:Female; D007114:Immunization; D007071:Immunoglobulin A, Secretory / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000008:administration & dosage; D012703:Serotyping; D013295:Streptococcus mutans / Q000145:classification / Q000276:immunology*
D000942:Antigens, Bacterial; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D002772:Cholera Toxin