Quantitation of free sulfate and total sulfoesters in human breast milk by ion chromatography.
A method for the assay of free sulfate and total sulfoesters in human breast milk by ion chromatography is described. After incubation in 1 M hydrochloric acid at 95 degrees C for 90 min, hydrolytic cleavage of sulfoester standards was essentially complete. The increase in free sulfate after hydrolysis was used as a measure of total acid-labile sulfoesters. We found that this fraction [222 +/- 16 mumol/l (mean +/- standard error), n = 29] comprised 87% of the total sulfate in mature milk. Free sulfate (35 +/- 3 mumol/l) therefore makes only a small contribution to the total sulfate pool available to human infants.
McPhee
M D
MD
I.W.K. Children's Hospital, Halifax, Nova Scotia, Canada.
Atkinson
S A
SA
Cole
D E
DE
D002852:Chromatography, Ion Exchange; D004952:Esters; D005260:Female; D006801:Humans; D008895:Milk, Human / Q000032:analysis*; D013431:Sulfates / Q000032:analysis*
D004952:Esters; D013431:Sulfates