In situ hybridization analysis of isodicentric X-chromosomes with short arm fusion.
We present here an alternative approach to the study of mosaic cell lines containing dicentric chromosomes. The approach is based on chromosome-specific non-radioactive in situ hybridization with centromere (alpha satellite DNA) probes. The hybridization analysis may be used as an alternative to the C-band analysis, while at the same time to some extent replacing the Q-band analysis as well. The advantage of using in situ hybridization is mainly that it allows the very fast screening of a large number of metaphases. We illustrate this new application of the technique by using it for the analysis of two cases of isodicentric X-chromosomes. The approach is expected to be generally applicable, so that it may be applied to the scoring of other types of chromosomal mosaicism as well.
Koch
J E
JE
Institute of Human Genetics, University of Aarhus, Denmark.
Kølvraa
S
S
Hertz
J M
JM
Rasmussen
K
K
Gregersen
N
N
Fly
G F
GF
Bolund
L A
LA
D002648:Child; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002874:Chromosome Mapping; D003582:Cytogenetics / Q000379:methods* / Q000592:standards; D015342:DNA Probes; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D009030:Mosaicism / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D014960:X Chromosome / Q000648:ultrastructure*
D015342:DNA Probes