Determination of membrane lipid phase transition temperature from 13C-NMR intensities.
A new and simple approach for the determination of the temperature of gel-to-liquid crystalline phase transitions (Tc) of biological (chloroplast) membrane lipids from 13C-NMR resonance intensities is proposed. The variation of intensity of a temperature-sensitive NMR resonance is monitored by recording the spectra of the sample at a range of temperatures. From such a series of spectra recorded at different temperatures, a temperature-insensitive resonance is located. Then the ratio of the intensity of the temperature-sensitive to the intensity of the temperature-insensitive resonance is calculated from each spectrum to even out the procedural error, if any. The values of this ratio at different temperatures, when plotted against sample temperature, shows a break at Tc as confirmed by spin label ESR studies.
YashRoy
R C
RC
Biology Department, Carleton University and N.R.C., Ottawa, Canada.
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002736:Chloroplasts; D009682:Magnetic Resonance Spectroscopy; D008560:Membrane Fluidity; D008563:Membrane Lipids; D013696:Temperature
D008563:Membrane Lipids