[The anchoring of newly synthesized adenovirus DNA to the nuclear matrix].
After adenovirus infected HeLa cells were pulse labeled and pulse-chase labeled with 3H-thymidine, the nuclear matrix and DNA remaining tightly bound to the matrix were obtained by sequential cell fractionation. Measuring the radioactivity of labeled DNA indicated that newly synthesized viral DNA specifically attach to the nuclear matrix and the amount of binding DNA is in direct proportion to the viral DNA replication activity: then the DNA gradually detach from the matrix and are involved in viral assembly. Electron microscopic autoradiography of the extracted cells showed the virion and viral DNA associated with the nuclear matrix, and thus further confirmed the anchoring of newly synthesized viral DNA to the nuclear matrix.
Chen
F
F
Department of Biology, Peking University, Beijing.
Xu
L Z
LZ
Ding
M X
MX
Zhai
Z H
ZH
D000260:Adenoviruses, Human / Q000378:metabolism*; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D015530:Nuclear Matrix / Q000382:microbiology
D004279:DNA, Viral