Electron microscopic demonstration of sialic acid residues in the organ of Corti with lectin from Limax flavus.
The localization of sialic acid (N-acetyl- and N-glyconylneuraminic acid) in the organ of Corti of the mongolian gerbil was examined with electron microscopy by postembedding labeling using Limax flavus agglutinin and feutin-gold. Gold labeling was observed on the fibrous structure of the tectorial membrane, the basilar membrane and the spiral limbus. The labeling was also observed on the cuticular plate of the hair cells, the head and cone of the pillar cells and the head plate of Deiters' cells and other supporting cells. Collagen or cytoskeletal glycoproteins are suggested to be the source of the sialic acid in these structures.
Tachibana
M
M
Department of Otolaryngology, Meiji College of Oriental Medicine, Kyoto, Japan.
Morioka
H
H
Sjöbäck
D B
DB
Wersäll
J
J
D000818:Animals; D005260:Female; D005849:Gerbillinae; D006046:Gold; D037102:Lectins; D008297:Male; D008854:Microscopy, Electron; D019158:N-Acetylneuraminic Acid; D009925:Organ of Corti / Q000378:metabolism*; D037121:Plant Lectins; D012794:Sialic Acids / Q000378:metabolism*
D037102:Lectins; C042962:Limax flavus agglutinin; D037121:Plant Lectins; D012794:Sialic Acids; D006046:Gold; D019158:N-Acetylneuraminic Acid