Covalently linked peptides for enzyme-linked immunosorbent assay.
A general method is described, by which synthetic peptides are covalently linked via their carboxyl group to microtiter plates (CovaLink) for enzyme-linked immunosorbent assay (ELISA). Plates were prepared by this method with an angiotensin II peptide and with an HIV-2 peptide and attachment detected by rabbit anti-angiotensin serum and with a positive serum from an HIV-2-infected patient, respectively, using the common ELISA procedure in the last steps. The method is simple to perform, it constitutes an alternative to the common ELISA method, and eliminates the risk of inadvertent loss of peptide during the procedure. The method is highly reproducible and has a high sensitivity. It may be used for either antigen or antibody detection.
Søndergård-Andersen
J
J
Research Center for Medical Biotechnology, Statens Seruminstitut, Copenhagen, Denmark.
Lauritzen
E
E
Lind
K
K
Holm
A
A
D000804:Angiotensin II / Q000032:analysis; D002264:Carboxylic Acids; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D015488:HIV Antigens / Q000032:analysis; D015658:HIV Infections / Q000276:immunology; D015498:HIV-2 / Q000276:immunology; D006801:Humans; D010455:Peptides / Q000032:analysis* / Q000276:immunology; D010969:Plastics; D012191:Retroviridae Proteins / Q000032:analysis
D002264:Carboxylic Acids; D015488:HIV Antigens; D010455:Peptides; D010969:Plastics; D012191:Retroviridae Proteins; D000804:Angiotensin II