Comparative study of colorimetric DNA hybridization method and conventional culture procedure for detection of Salmonella in foods.
A second generation nucleic acid hybridization assay has been developed and evaluated against the conventional culture method for detection of salmonellae in foods. The assay involves a liquid hybridization with Salmonella-specific oligonucleotide probes, capture of probe:target hybrids onto a solid support (plastic dipstick), and a colorimetric end point detection. The assay can be completed in 2.5 h, following approximately 44 h of culture enrichment. One thousand samples representing 20 food types were analyzed in parallel by both methods. Samples included uninoculated test product, and product inoculated with Salmonella at 2 levels. Eighteen Salmonella serotypes were used as inocula. The data demonstrate that the colorimetric hybridization method and the conventional culture method are equivalent in their ability to detect Salmonella contamination of foods.
Chan
S W
SW
Gene-Trak Systems, Framingham, MA 01701.
Wilson
S G
SG
Vera-Garcia
M
M
Whippie
K
K
Ottaviani
M
M
Whilby
A
A
Shah
A
A
Johnson
A
A
Mozola
M A
MA
Halbert
D N
DN
D000818:Animals; D003124:Colorimetry; D004269:DNA, Bacterial / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D009693:Nucleic Acid Hybridization; D012475:Salmonella / Q000302:isolation & purification*
D004269:DNA, Bacterial