Deoxyribonucleic acid analysis of the zinc finger Y gene in 45,X/46,XY subjects.
The deoxyribonucleic acid from nine subjects with a 45,X/46,XY karyotype with a cytogenetically intact Y chromosome and phenotypically presenting with bilateral streak gonads, streak and testis, or bilateral scrotal testes along with a control male and female were analyzed for the presence of the zinc finger Y sequence through the molecular probe pDP1007. This particular probe is thought to constitute part of the putative testicular-determining factor gene. All the study subjects demonstrated the presence of zinc finger Y. Laser densitometry studies confirmed a correlation between the intensity of the zinc finger Y band and the percentage of Y cell lines. This study supports the fact that individuals with mixed gonadal dysgenesis and cytogenetically intact Y chromosomes will tend to have intact zinc finger Y sequences.
Plouffe
L
L
Department of Obstetrics and Gynecology, Medical College of Georgia, Augusta 30912.
Behzadian
A
A
Tho
S P
SP
McDonough
P G
PG
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D004247:DNA / Q000032:analysis*; D005260:Female; D005796:Genes; D005837:Genitalia, Male / Q000002:abnormalities*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007621:Karyotyping; D008297:Male; D015335:Molecular Probes; D009030:Mosaicism; D009693:Nucleic Acid Hybridization; D014998:Y Chromosome
D015335:Molecular Probes; D004247:DNA