Suppression of spontaneous maturation of isolated cumulus-free mouse oocytes by a calmodulin antagonist.
Oocytes isolated from antral follicles undergo spontaneous maturation when cultured in vitro. W7, a calmodulin antagonist, at concentration of more than 50 microM blocked the occurrence of spontaneous germinal vesicle breakdown (GVBD) in isolated cumulus-free mouse oocytes. The inhibition of maturation was observed in more than 90% of oocytes when W7 was added within 15 min after the initiation of incubation of the oocytes. The block was partially reversible. Hypoxanthine, estradiol-17 beta, testosterone and progesterone did not influence the inhibition induced with W7. The present results suggest that calmodulin is involved in the early stage of mouse oocyte maturation.
Sato
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Department of Animal Science, Faculty of Agriculture, Kyoto University.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002147:Calmodulin / Q000037:antagonists & inhibitors*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005498:Follicular Phase / Q000187:drug effects; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000494:pharmacology; D066298:In Vitro Techniques; D051379:Mice; D009866:Oogenesis / Q000187:drug effects*; D011374:Progesterone / Q000494:pharmacology; D013449:Sulfonamides / Q000494:pharmacology*; D013739:Testosterone / Q000494:pharmacology
D002147:Calmodulin; D007042:Hypoxanthines; D013449:Sulfonamides; D019271:Hypoxanthine; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; C017967:W 7; C034700:N-(6-aminohexyl)-1-naphthalenesulfonamide; D002118:Calcium