High-resolution proton nuclear magnetic resonance characterization of seminolipid from bovine spermatozoa.
The high-resolution one- and two-dimensional proton nuclear magnetic resonance (1H-NMR) characterization of seminolipid from bovine spermatozoa is presented. The 1H-NMR data was confirmed by gas-liquid chromatography-mass spectrometric analysis of the partially methylated alditol acetates of the sugar unit, mild alkaline methanolysis of the glyceryl ester, mobility on normal phase and diphasic thin-layer chromatography (HPTLC), and fast atom bombardment mass spectrometry (FAB-MS). The structure of the molecule corresponds to 1-O-hexadecyl-2-O-hexadecanoyl-3-O-beta-D-(3'-sulfo)-galactopyranosyl- sn-glycerol.
Alvarez
J G
JG
Department of Physiology, University of Pennsylvania, School of Medicine, Philadelphia 19104.
Storey
B T
BT
Hemling
M L
ML
Grob
R L
RL
D000818:Animals; D002417:Cattle; D006017:Glycolipids / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008968:Molecular Conformation; D013094:Spermatozoa / Q000032:analysis*
D006017:Glycolipids; C009233:seminolipid