[Effects of remantadine on fusion of lipid membranes of influenza A virus with plasma and internal membranes of lymphoblastic cells].
The method of fluorescence dequenching was used to study the interaction between influenza virus A/Krasnodar/101/59 and Namalwa and Raji lymphoblastoid cells. Experiments with endocytosis inhibitors and fluorescence quenchers have shown that at pH = 5.0 the virus lipid envelopes are fused with the plasma membranes of the cells, and at pH = 7.4 the virus lipid envelopes are fused with the internal, presumably endosomal, membranes of the cells. Remantadine at a concentration of 50-1000 micrograms/ml did not influence the fusion of virus lipid envelopes with intracellular membranes at pH = 7.4 whereas at pH = 5.0 it inhibited, beginning from 25 micrograms/ml concentration, the fusion of virus lipid envelopes with the plasma membranes of cells.
Leneva
I A
IA
Gulak
P V
PV
Dubrov
Iu N
IuN
Sobolev
A S
AS
D000218:Adamantane / Q000031:analogs & derivatives*; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects*; D002642:Chick Embryo; D004705:Endocytosis; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D009980:Influenza A virus / Q000187:drug effects*; D008563:Membrane Lipids; D012299:Rimantadine / Q000494:pharmacology*
D005456:Fluorescent Dyes; D008563:Membrane Lipids; D012299:Rimantadine; D000218:Adamantane
Deĭstvie remantadina na sliianie lipidnoĭ obolochki virusa grippa A s plazmaticheskimi i vnutrennimi membranami v limfoblastoidnykh kletkakh.