title: Enhancing effect of centrifugation on isolation of influenza virus from clinical specimens.
abstract: The use of centrifugation (700 x g, 60 min) in a plaque assay markedly increased (mean, 2.9-fold) the infectivity of all 42 influenza virus strains tested, compared with no centrifugation. Of 13 influenza virus strains isolated from 390 clinical specimens, 9 (69%) were efficiently isolated by the centrifugation assay compared with conventional culture methods. The centrifugation assay may be useful for isolating the influenza virus from clinical specimens.
authors.affiliation: Division of Microbiology, Hiroshima Prefectural Institute of Public Health, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Seno
authors.forename: Y
authors.initials: Y
authors.lastname: Kanamoto
authors.forename: S
authors.initials: S
authors.lastname: Takao
authors.forename: N
authors.initials: N
authors.lastname: Takei
authors.forename: S
authors.initials: S
authors.lastname: Fukuda
authors.forename: H
authors.initials: H
authors.lastname: Umisa
mesh_terms: D002498:Centrifugation; D005069:Evaluation Studies as Topic; D006801:Humans; D009975:Orthomyxoviridae / Q000302:isolation & purification*; D010948:Viral Plaque Assay; D014773:Virology / Q000379:methods*