Sugar competition assays reveal high affinity receptors for Erythrina cristigalli lectin on feline monocytes.
An examination of fluorescein-labelled Erythrina cristigalli lectin (FITC-ECL) staining on feline mononuclear cells (MNC) using fluorescent microscopy and a novel sugar titration competition assay revealed that monocytes (MO) were stained brighter by FITC-ECL than were lymphocytes (LYM). When MNC were stained with FITC-ECL in the presence of 400 mM or greater D-galactose, analysis by flow cytometry revealed continued MO staining while LYM were negative. MO expressed a larger quantity of carbohydrate receptors (CHO-R) for ECL than did LYM. The CHO-R expressed on MO were mostly protease-insensitive and uncapped by sialic acid residues. All of the CHO-R on LYM were protease-sensitive and many were capped by sialic acid residues. A combined labelling of MNC for non-specific esterase staining, latex bead ingestion and FITC-ECL staining in the presence of 400 mM D-galactose confirmed that FITC-ECL specifically stains MO in the presence of high sugar competitor concentrations.
Whitehurst
C E
CE
Department of Pediatrics, All Children's Hospital, University of South Florida, St. Petersburg 33701.
Day
N K
NK
Gengozian
N
N
D000818:Animals; D050260:Carbohydrate Metabolism; D002415:Cats; D004895:Erythrina; D005434:Flow Cytometry; D037102:Lectins / Q000378:metabolism; D009000:Monocytes / Q000378:metabolism*; D009439:Neuraminidase / Q000494:pharmacology; D037121:Plant Lectins; D010946:Plants, Medicinal; D011402:Pronase / Q000494:pharmacology; D011975:Receptors, Mitogen / Q000378:metabolism*
D037102:Lectins; D037121:Plant Lectins; D011975:Receptors, Mitogen; C045629:erythrina lectin; D009439:Neuraminidase; D011402:Pronase