[Effect of anti-ulcer drugs and PGE2 on the proliferation of rat gastric cultured cells].
The effects of anti-ulcer drugs and PGE2 on the proliferation of rat gastric cultured cells were investigated. To study the cell proliferation, the rate of incorporation of 3H-thymidine into the cultured cells was measured. ULTROSER G, corresponding to fetal bovine serum, was found to dose-dependently increase the incorporation of 3H-thymidine into the cultured cells at a dose range of 1-4%. The incorporation of 3H-thymidine was highest at 36 hr after the inoculation of cells. Therefore, the effect of the drugs on the proliferation was performed under the condition of 2% ULTROSER G and evaluated at 36 hr. Aldioxa, cetraxate HCl, cimetidine (10(-7)-10(-5) M) and PGE2 (10(-8)-10(-6) M) had no effect on the cell proliferation, while indomethacin (0.35-1.41 x 10(-3) M) inhibited the proliferation in a dose-dependent manner. Aldioxa, cetraxate HCl (10(-5) M) and PGE2 (10(-6), 10(-8) M) antagonized the inhibitory effect by indomethacin at a concentration of 0.5 x 10(-3) M, but cimetidine (10(-7)-10(-5) M) did not. From the above results, it was concluded that this method was useful for investigating the effect of drugs on the proliferation of gastric cultured cells.
Uchida
M
M
Research and Development Division, Grelan Pharmaceutical Co., Ltd., Tokyo, Japan.
Nishimura
N
N
Misaki
N
N
Ikarashi
Y
Y
Kobayashi
M
M
Kawano
O
O
D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D001802:Blood Substitutes / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002927:Cimetidine / Q000494:pharmacology*; D015232:Dinoprostone / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005753:Gastric Mucosa / Q000166:cytology* / Q000378:metabolism; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D009930:Organic Chemicals; D051381:Rats; D011919:Rats, Inbred Strains; D013936:Thymidine / Q000378:metabolism; D014148:Tranexamic Acid / Q000031:analogs & derivatives / Q000494:pharmacology
D000897:Anti-Ulcer Agents; D001802:Blood Substitutes; D009930:Organic Chemicals; C044609:Ultroser G; C000665:cetraxate; D014148:Tranexamic Acid; D002927:Cimetidine; D015232:Dinoprostone; D013936:Thymidine; D007213:Indomethacin