Sister chromatid exchange and proliferation pattern in lymphocytes from newborns, elderly subjects and in premature aging syndromes.
Sister chromatid exchange (SCE) frequency and cell proliferation were examined in lymphocyte cultures from a group of newborns, a group of elderly subjects and from patients with syndromes who exhibit progeriform characteristics (progeria, Cockayne syndrome, Rothmund-Thomson syndrome and Christ-Siemens-Touraine syndrome) by using the bromodeoxyuridine incorporation differential staining technique. We observed a significantly increase in basal SCE frequency and a less intensive cell proliferation in cultures from elderly subjects than from newborns, as shown by the significant increase in percentage of cells in first generation simultaneous with a reduction of cells in more advanced generations. Lymphocyte cultures from each one of the patients studied also showed a decreased cell proliferation in relation to their respective control and to newborn cultures. Each of these syndromes showed higher baseline SCE levels than the control and than the newborn and elderly groups. Only the patient with progeria showed values similar to those for the elderly group. Thus, in addition to showing clinical characteristics similar to those observed during the normal aging process, these progeriform syndromes also show cytogenetic characteristics similar to those of older individuals.
Melaragno
M I
MI
Disciplina de Genetica, Escola Paulista de Medicina, Sao Paulo SP, Brazil.
Smith
M de A
Mde A
D000368:Aged; D000375:Aging / Q000502:physiology*; D002455:Cell Division; D003057:Cockayne Syndrome / Q000235:genetics / Q000473:pathology; D004476:Ectodermal Dysplasia / Q000235:genetics / Q000473:pathology; D006801:Humans; D007231:Infant, Newborn / Q000502:physiology*; D008214:Lymphocytes / Q000166:cytology / Q000502:physiology*; D011371:Progeria / Q000235:genetics* / Q000473:pathology; D012854:Sister Chromatid Exchange