title: A general method of optimizing automated DNA synthesis to decrease chemical consumption to less than half.
abstract: An automated DNA synthesis protocol (FC3) has been optimized to decrease the cost of chemical consumption by two- to threefold. Anion-exchange chromatography at pH 12.5 in a Mono Q (Pharmacia) column was used to analyze the oligonucleotides we synthesized. From these analyses, we formulated the FC3 program and a procedure by which a DNA synthesizer designer or operator can use 18-base-long homopolymers of A, G, C, and T to optimize the reaction times, reagent concentrations, solvent wash conditions, and the many steps in the synthesis cycle.
authors.affiliation: Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
authors.forename: A T
authors.initials: AT
authors.lastname: Yeung
authors.forename: C G
authors.initials: CG
authors.lastname: Miller
mesh_terms: D001331:Automation / Q000191:economics / Q000379:methods; D001483:Base Sequence; D002852:Chromatography, Ion Exchange; D004247:DNA / Q000138:chemical synthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000302:isolation & purification
chemical_list: D009841:Oligonucleotides; D004247:DNA