title: Evaluation of pyrimido[5,4-d]pyrimidine derivatives as peroxyoxalate chemiluminescence reagents using a flow injection system.
abstract: Eighteen kinds of pyrimido[5,4-d]pyrimidines together with several commercially available fluorescent compounds such as perylene, Rhodamine B, etc., were evaluated as the reagents for a peroxyoxalate chemiluminescence (CL) detection system by using a flow injection method. The peroxyoxalate CL reaction employed consists of bis(2,4,6-trichlorophenyl)oxalate, hydrogen peroxide, triethylamine, and a fluorophore. Under the conditions used, 2,6-bis[di-(2-hydroxyethyl)amino]-4,8- dipiperidinopyrimido[5,4-d]pyrimidine (Dipyridamole) and 2,4,6,8-tetrathiomorpholinopyrimido[5,4-d]pyrimidine (1i) gave very intense chemiluminescence intensities which were larger than those of any other commercially available fluorescent compounds tested (e.g., 10 times larger than that of perylene).
authors.affiliation: Faculty of Pharmaceutical Sciences, Nagasaki University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nakashima
authors.forename: K
authors.initials: K
authors.lastname: Maki
authors.forename: S
authors.initials: S
authors.lastname: Akiyama
authors.forename: K
authors.initials: K
authors.lastname: Imai
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008163:Luminescent Measurements; D010070:Oxalates / Q000032:analysis* / Q000378:metabolism; D011743:Pyrimidines
chemical_list: D010070:Oxalates; D011743:Pyrimidines; C044622:peroxyoxalate