K-sam, an amplified gene in stomach cancer, is a member of the heparin-binding growth factor receptor genes.
DNA fragments amplified in a stomach cancer-derived cell line, KATO-III, were previously identified by the in-gel DNA renaturation method, and a 0.2-kilobase-pair fragment of the amplified sequence was subsequently cloned. By genomic walking, a portion of the exon of the gene flanking this 0.2-kilobase-pair fragment was cloned, and the gene was designated as K-sam (KATO-III cell-derived stomach cancer amplified gene). The K-sam cDNAs, corresponding to the 3.5-kilobase K-sam mRNA, were cloned from the KATO-III cells. Sequence analysis revealed that this gene coded for 682 amino acid residues that satisfied the characteristics of the receptor tyrosine kinase. The K-sam gene had significant homologies with bek, FLG, and chicken basic fibroblast growth factor receptor gene. The K-sam gene was amplified in KATO-III cells with the major transcript of 3.5-kilobases in size. This gene was also expressed in some other stomach cancer cells, a small cell lung cancer, and germ cell tumors.
Hattori
Y
Y
Genetics Division, National Cancer Center Research Institute, Tokyo, Japan.
Odagiri
H
H
Nakatani
H
H
Miyagawa
K
K
Naito
K
K
Sakamoto
H
H
Katoh
O
O
Yoshida
T
T
Sugimura
T
T
Terada
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D004273:DNA, Neoplasm / Q000235:genetics; D000091344:Filaggrin Proteins; D005784:Gene Amplification; D006133:Growth Substances / Q000378:metabolism; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D005810:Multigene Family; D009368:Neoplasm Transplantation; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D011975:Receptors, Mitogen / Q000235:genetics*; D040262:Receptors, Vascular Endothelial Growth Factor; D012689:Sequence Homology, Nucleic Acid; D013274:Stomach Neoplasms / Q000235:genetics*; D014183:Transplantation, Heterologous
D004273:DNA, Neoplasm; C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D006133:Growth Substances; D012334:RNA, Neoplasm; D011975:Receptors, Mitogen; D040262:Receptors, Vascular Endothelial Growth Factor