Prolactin stimulates milk protein promoter in CHO cells cotransfected with prolactin receptor cDNA.
A functional biological system was developed by cotransfecting mammalian cell lines with the cDNA of the prolactin receptor (PRL-R) and a fusion gene containing the promoter of the milk protein, ovine beta-lactoglobulin linked to the coding sequence of the chloramphenicol acetyltransferase (CAT) gene. Surprisingly, this system is effective even if a non-mammary cell line is used, since Chinese hamster ovary (CHO) cells transfected both transiently and stably with PRL-R cDNA respond to PRL, as observed by stimulation of the reporter gene. This newly developed system should help precisely define the functional domains of both the PRL-R molecule and of the regulatory elements of a PRL target gene.
Lesueur
L
L
Unité d'Endocrinologie Moléculaire, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
Edery
M
M
Paly
J
J
Clark
J
J
Kelly
P A
PA
Djiane
J
J
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004247:DNA / Q000235:genetics*; D015870:Gene Expression; D007782:Lactoglobulins / Q000235:genetics*; D011388:Prolactin / Q000494:pharmacology*; D011401:Promoter Regions, Genetic; D011981:Receptors, Prolactin / Q000235:genetics*; D014162:Transfection
D007782:Lactoglobulins; D011981:Receptors, Prolactin; D011388:Prolactin; D004247:DNA