title: Anti-human factor IX monoclonal antibodies specific for calcium ion-induced conformations.
abstract: Four monoclonal antibodies have been produced, which are specific for the Ca2+ or Sr2(+)-induced conformation of human factor IX. Certain, but not all, gamma-carboxy-glutamic acid residues in factor IX are involved in the epitope expression together with the conformation stabilized by the adjacent region of Gla-domain and a disulfide bridge. All the antibodies interfered with the binding of factor IX to phospholipids and inhibited the procoagulant activity of factors IX and IXa beta.
authors.affiliation: Institute of Hematology, Jichi Medical School, Tochigi, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sugo
authors.forename: J
authors.initials: J
authors.lastname: Mizuguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Kamikubo
authors.forename: M
authors.initials: M
authors.lastname: Matsuda
mesh_terms: D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D001780:Blood Coagulation Tests; D002118:Calcium / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D005164:Factor IX / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D010743:Phospholipids / Q000378:metabolism; D011487:Protein Conformation
chemical_list: D000911:Antibodies, Monoclonal; D010743:Phospholipids; D005164:Factor IX; D002118:Calcium