Human synovial cells secrete a 39 kDa protein similar to a bovine mammary protein expressed during the non-lactating period.
By SDS/PAGE analysis we have observed that human synovial cell monolayers secrete a prominent 39 kDa protein which could not be detected in skin and lung fibroblasts. This protein was purified to homogeneity by heparin-Sepharose chromatography and reverse-phase h.p.l.c. The N-terminal sequence was found to be almost identical to that of a recently described bovine protein detected in the mammary secretions during the involutionary phase of the lactational cycle. Characterization of this 39 kDa protein may provide a useful marker for classification of connective tissue cells.
Nyirkos
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Shriners Hospital, Joint Diseases Laboratory, Montreal, Quebec, Canada.
Golds
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D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011506:Proteins / Q000378:metabolism*; D013583:Synovial Membrane / Q000378:metabolism*
D000596:Amino Acids; D011506:Proteins