[Effect of a new positive inotropic agent (OPC-8212) on intracellular potential of isolated canine ventricular papillary muscle].
This study adopted intracellular microelectrode technic to observe the effect of OPC-8212 (Vesnarinone) on intracellular potential of isolated canine ventricular papillary muscle. The record and data processing were completed by computerized intellective instrument designed by ourselves. The results of intracellular record in 14 canine ventricular papillary muscle showed no significant change in the resting potential (RP), amplitude of action potential (APA) and maximum depolarize velocity of 0 phase (Vmax). But significantly increased whole duration of action potential (APT). The duration of repolarization to 1/3 (APD 1/3) and to 2/3 (APD 2/3) significantly increased too. The plateau phase raised. These results suggest that K+ permeability at resting myocardiac cell membrane may have not been effected by OPC-8212. Activation of the fast sodium channel may have not been effected too. The results of our studies also suggest that inward flow of Ca2+ increased. The delayed outward K+ (Ik) and the inward, rectifying K+ current (Ik1) possible decreased. Thus, the OPC-8212 might become a nonglucoside and noncatecholamine new positive inotropic drug.
Zha
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Lan
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Zhou
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Yang
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D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology*; D004285:Dogs; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D008839:Microelectrodes; D009200:Myocardial Contraction / Q000187:drug effects*; D010210:Papillary Muscles / Q000502:physiology; D011719:Pyrazines; D011804:Quinolines / Q000494:pharmacology*; D013268:Stimulation, Chemical
D002316:Cardiotonic Agents; D011719:Pyrazines; D011804:Quinolines; C041667:vesnarinone