title: A novel mast cell growth factor (MCGF-3) produced by marrow-adherent cells that synergizes with interleukin 3 and interleukin 4.
abstract: A clonal marrow-adherent stromal cell line, +/+-1 LDA11, was derived and found to produce hemopoietic stimulatory activity for an interleukin 3 (IL-3)-dependent mast cell line, NFS/N1. This factor-dependent mast cell line displayed restricted growth factor responsiveness to only IL-3, interleukin 4 (IL-4), and the stromal cell-produced factor. The factor produced by stromal cells was distinguished from IL-3 and IL-4 and was characterized biochemically. This factor appears to be a novel mast cell growth factor (MCGF-3) capable of synergizing with IL-3 and IL-4. It may have broader reactivity in hemopoiesis than simply IL-3-dependent mast cells, and it may prove relevant to stromal cell-mediated hemopoiesis.
authors.affiliation: Hematology/Oncology Division, Veterans Administration Medical Center, Indianapolis, Indiana.
authors.forename: H S
authors.initials: HS
authors.lastname: Boswell
authors.forename: D Y
authors.initials: DY
authors.lastname: Mochizuki
authors.forename: G S
authors.initials: GS
authors.lastname: Burgess
authors.forename: S
authors.initials: S
authors.lastname: Gillis
authors.forename: E B
authors.initials: EB
authors.lastname: Walker
authors.forename: D
authors.initials: D
authors.lastname: Anderson
authors.forename: D E
authors.initials: DE
authors.lastname: Williams
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000502:physiology*; D001854:Bone Marrow Cells; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line; D004357:Drug Synergism; D006133:Growth Substances / Q000494:pharmacology*; D006410:Hematopoiesis; D007377:Interleukin-3 / Q000494:pharmacology*; D015847:Interleukin-4 / Q000494:pharmacology*; D008407:Mast Cells / Q000166:cytology*; D051379:Mice
chemical_list: D006133:Growth Substances; D007377:Interleukin-3; D015847:Interleukin-4