One amino acid change in rat SMR1 polypeptide induces a 1 kDa difference in its apparent molecular mass determined by electrophoretic analysis.
SMR1 is a male-specific, 19 kDa, in vitro translation product of Wistar rat submaxillary glands, which may be the precursor of a small hormone resembling the TRH. In Sprague-Dawley and Fischer rats, instead of SMR1, a male-specific 18 kDa polypeptide may be found. We have cloned the cDNA encoding the 18 kDa polypeptide. We show that the 19 and the 18 kDa polypeptides have the same sequence except for one amino and change.
Rosinski-Chupin
I
I
Département d'Immunologie, Institut Pasteur, Paris, France.
Rougeon
F
F
D000818:Animals; D003001:Cloning, Molecular; D004247:DNA; D004586:Electrophoresis; D005260:Female; D066298:In Vitro Techniques; D008970:Molecular Weight; D009154:Mutation; D009842:Oligopeptides; D010452:Peptide Biosynthesis; D010455:Peptides / Q000235:genetics*; D011110:Polymorphism, Genetic; D051381:Rats; D011919:Rats, Inbred Strains; D013363:Submandibular Gland / Q000378:metabolism*
D009842:Oligopeptides; D010455:Peptides; D004247:DNA