A sensitive enzyme-linked immunosorbent assay used to differentiate type AB specimen from a mixture of type A and type B specimens.
A sensitive enzyme-linked immunosorbent assay (ELISA) for detection of ABO blood substances in human body fluids was devised. The ELISA plates coated with purified human anti-A or anti-B serum effectively captured the blood substances, and these were then analysed by the combination of rabbit anti-A and goat anti-B. This capture ELISA could differentiate the type AB specimen from a mixture of the type A and the type B specimens, and the method was applied to rape cases to make the ABO typing of the criminal.
Sato
M
M
Department of Legal Medicine, Nagoya University School of Medicine, Japan.
Higashide
K
K
Katsumata
Y
Y
D000017:ABO Blood-Group System; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D001826:Body Fluids / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D008297:Male; D012661:Semen / Q000032:analysis; D014621:Vagina / Q000378:metabolism
D000017:ABO Blood-Group System