[Multiple drug resistance and differentiation in the population of transformed dog kidney cells MDCK].
Sublines of the canine kidney epithelium cells (MDCK) resistant to various colchicine doses (0.3-5.0 mcg.ml-1) were obtained by multistep selection. The new lines were shown to possess multidrug-resistance (MDR) by means of Rhodamine 123 staining. The initial steps of resistance (2 mcg.ml-1, 300-fold increase in resistance) are evidently due to enhancement of mdr gene transcription. Approximately 4-fold gene amplification was revealed in the cells resistant to 3 mcg.ml-1 of colchicine. All the drug-resistant lines tested were found to be more prone to differentiation than the wild type cells (both spontaneous and induced by several drugs such as DMSO, cAMP and others). The data suggest that the selection for overexpression of mdr gene results in selection of variants that are more capable of differentiation than the rest of the population.
Stavrovskaia
A A
AA
Stromskaia
T P
TP
Chernova
O B
OB
Dzhuraeva
F Kh
FKh
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002461:Cell Line, Transformed; D003078:Colchicine / Q000494:pharmacology; D004247:DNA / Q000235:genetics; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004285:Dogs; D004351:Drug Resistance / Q000235:genetics*; D015870:Gene Expression; D007668:Kidney; D010641:Phenotype
D015124:8-Bromo Cyclic Adenosine Monophosphate; D004247:DNA; D003078:Colchicine; D004121:Dimethyl Sulfoxide
Mnozhestvennaia lekarstvennaia ustoĭchivost' i differentsirovka v populiatsii transformirovannykh kletok pochki sobaki MSCK.