title: Structure and sequence determination of the gene encoding human salivary statherin.
abstract: Human statherin (STT) is a low-Mr (43 amino acids) acidic phosphoprotein secreted mainly by salivary glands. It acts as an inhibitor of precipitation of Ca.phosphate salts in the oral cavity. DNA (12.2 kb) was isolated from human genomic phage lambda libraries as a series of overlapping clones, and the nucleotide sequence of the STT-encoding gene (STT) was determined. The transcribed region spans 6.5 kb and contains six exons and five introns. Upstream DNA (1.6 kb) was also sequenced and a number of possible regulatory elements were identified. The exon-intron boundaries of the STT gene roughly coincide with the protein-coding regions of the mRNA and with the functional domains of STT. This pattern of organization has been seen in a variety of eukaryotic genes and is consistent with the domain theory of gene evolution.
authors.affiliation: Department of Medical Genetics, University of Wisconsin, Madison 53706.
authors.forename: L M
authors.initials: LM
authors.lastname: Sabatini
authors.forename: Y Z
authors.initials: YZ
authors.lastname: He
authors.forename: E A
authors.initials: EA
authors.lastname: Azen
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004622:Embryo, Mammalian; D005091:Exons; D015723:Gene Library; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012469:Salivary Glands / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
chemical_list: C018149:STATH protein, human; D012471:Salivary Proteins and Peptides