A quick method for obtaining lymph-borne dendritic macrophages from rats.
After conventional cannulation of the thoracic duct (cisterna chyli) of rats, the mesenteric nodes were frozen solid by the application of solid carbon dioxide and a metal probe that had been cooled to the temperature of liquid nitrogen. This procedure destroyed the functional integrity of the node so that peripheral intestinal lymph, rich in dendritic macrophages flowed, unaltered, into the thoracic duct. In this way peripheral intestinal lymph could be collected immediately and the time-consuming and expensive two-stage procedure of conventional surgical lymphadenectomy was avoided.
Gyure
L A
LA
Experimental Unit, Institute of Cancer Research, Sutton, U.K.
Hall
J G
JG
D000818:Animals; D002469:Cell Separation / Q000379:methods; D003713:Dendritic Cells; D008196:Lymph / Q000166:cytology*; D008198:Lymph Nodes / Q000166:cytology; D008264:Macrophages; D008643:Mesentery / Q000166:cytology; D051381:Rats