Effect of aging on calcium cytosolic concentration in rat cortical synaptosomes.
Cytosolic Ca2+ concentrations [( Ca2+]i) were measured in synaptosomes prepared from the cerebral cortex of 3, 16 and 24 month-old male Charles River Wistar rats. Electron microscopy examination demonstrated no morphological differences between the synaptosomes prepared from 3 and 24 month-old rats. Age did not modify [Ca2+]i, as measured by the QUIN 2 technique, both at rest and immediately after depolarization with 50 mM K+. The Ca2+ load following depolarization was cleared in about 13 min in the 3 month-old rats. The rate of clearance was significantly slower both in the (p less than 0.01) and in the 24 month-old (p less than 0.0001). A prolonged calcium influx may be responsible for the slower clearance of Ca2+ load in aged rats.
Pepeu
G
G
Dipartimento di Farmacologia Preclinica e Clinica, Università degli Studi, Florence, Italy.
Giovannelli
L
L
D000375:Aging / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D008297:Male; D051381:Rats; D012964:Sodium / Q000378:metabolism; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*; D014700:Verapamil / Q000494:pharmacology
D012964:Sodium; D014700:Verapamil; D002118:Calcium