Electrophoretic comparison of the Leishmania vectors Phlebotomus papatasi and P. langeroni (Diptera: Psychodidae).
Cellulose acetate electrophoresis was employed to detect 22 different enzyme systems in laboratory-reared populations of the sympatric Leishmania vectors, Phlebotomus papatasi (Scopoli) and P. langeroni Nitzulescu. Electrophoretic conditions sensitive enough to permit as many as eight separate enzyme assays to be performed on individual specimens were developed. Under these conditions, 18 enzymes were detected with high resolution and regularity. Evidence was obtained which suggested that a number of enzymes in both species are under multilocus genetic control. Polymorphism was observed in 14 of 25 (56%) loci detected in P. papatasi and in eight of 24 (33%) loci detected in P. langeroni. Differences in electrophoretic profiles of malic enzyme, 6-phosphogluconate dehydrogenase, and fumarate hydratase were considered to be genetically fixed in both sexes of P. papatasi and P. langeroni. Their detection may permit an accurate and more rapid separation of these vectors in field collections.
Kassem
H A
HA
Medical Zoology Division, U.S. Naval Medical Research Unit, FPO, N.Y. 09527.
Fryauff
D J
DJ
el Sawaf
B M
BM
Shehata
M G
MG
Shoumar
N F
NF
D000818:Animals; D004588:Electrophoresis, Cellulose Acetate; D004798:Enzymes / Q000032:analysis*; D005260:Female; D007303:Insect Vectors / Q000201:enzymology*; D007896:Leishmaniasis / Q000635:transmission; D008297:Male; D010691:Phlebotomus / Q000201:enzymology*
D004798:Enzymes