Collagen gel cultures for detection of spared hematopoietic progenitors in Asta Z 7557 purged human marrows.
Asta-Z 7557, an in vitro active metabolite of cyclophosphamide, is used for human bone marrow purging in acute leukemias and solid tumors since it is more highly toxic to tumor than normal hematopoietic clonogenic cells. However, doses higher than 80 microM totally inhibit the growth of hematopoietic progenitors in semisolid assays, despite preservation of marrow repopulating ability in vivo. This discrepancy complicates assessment of the functional value of purged grafts. In 11 patients undergoing autologous bone marrow transplantation with 100 microM Asta-Z purged marrow, we investigated the advantages of the collagen gel culture system for detection of spared hematopoietic progenitors. This technique provides a suitable and convenient assay as compared with results with agar. In nine of 11 samples after 14 or 21 days of incubation, up to 100 colonies were observed, (64% monocytic, 27% granulocytic). Day-14 granulocytic colonies were mostly immature, suggesting that they derived from early CFU-GM or pre-CFU-GM. This development of early progenitors could account for the better sensitivity of this technique compared with agar or methylcellulose systems. The mechanism involved as well as the correlation between colony growth in collagen and clinical hematopoietic recovery is also considered.
Praloran
V
V
Laboratoire d'Hématologie, Centre Hospitalier Régional Universitaire de Nantes, France.
Dobo
I
I
Garand
R
R
Klausman
M
M
Naud
M F
MF
Milpied
N
N
Harousseau
J L
JL
D000293:Adolescent; D000328:Adult; D000362:Agar; D001853:Bone Marrow / Q000187:drug effects*; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation / Q000502:physiology*; D002449:Cell Aggregation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002648:Child; D003094:Collagen; D003114:Colony-Forming Units Assay / Q000379:methods*; D003520:Cyclophosphamide / Q000031:analogs & derivatives* / Q000494:pharmacology; D005782:Gels; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000502:physiology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000502:physiology; D013997:Time Factors
D005782:Gels; C037933:Asta Z 7557; D003520:Cyclophosphamide; D000362:Agar; D003094:Collagen