Nature of the organic cofactor of pig plasma benzylamine oxidase.
The identification of the organic cofactor of pig plasma benzylamine oxidase is described. Acid hydrolysis of the enzyme in argon in the presence of phenylhydrazine (6 h at 115 degrees C in 0.027 M sulphuric acid) allowed the isolation of an adduct which was purified by HPLC and identified as phenylhydrazone of pyridoxal by spectrophotometry, spectrofluorimetry and mass spectrometry.
Buffoni
F
F
Department of Pharmacology, University of Florence, Italy.
D000818:Animals; D001595:Benzylamine Oxidase / Q000097:blood* / Q000302:isolation & purification; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006868:Hydrolysis; D013058:Mass Spectrometry; D008995:Monoamine Oxidase / Q000097:blood*; D010659:Phenylhydrazines; D011732:Pyridoxal Phosphate / Q000097:blood* / Q000302:isolation & purification; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013552:Swine
D010659:Phenylhydrazines; C030299:phenylhydrazine; D011732:Pyridoxal Phosphate; D001595:Benzylamine Oxidase; D008995:Monoamine Oxidase