Rapid cloning and characterization of new chromosome 10 DNA markers by Alu element-mediated PCR.
Alu element-mediated polymerase chain reaction is a strategy for rapidly cloning and mapping human DNA markers from mixed DNA sources. A novel primer homologous to the 3' end of the human Alu repeat element provides the basis for preferential synthesis of human DNA fragments from human/rodent somatic cell hybrid DNA template. This approach has been used to isolate a series of new markers from chromosome 10. The Alu element-mediated PCR probes were regionally assigned on chromosome 10 by hybridization to Southern blots of Alu PCR-synthesized DNA derived from somatic cell hybrid template DNA. Alu element-mediated PCR is generally applicable and makes possible the analysis of complex genomes with a speed and sensitivity that has not been previously possible.
Brooks-Wilson
A R
AR
Department of Medical Genetics, University of British Columbia, Vancouver, Canada.
Goodfellow
P N
PN
Povey
S
S
Nevanlinna
H A
HA
de Jong
P J
PJ
Goodfellow
P J
PJ
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002879:Chromosomes, Human, Pair 10; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015723:Gene Library; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D013698:Templates, Genetic
D005819:Genetic Markers; D004247:DNA