A method for the study of fungal growth inhibition by plant proteins.
A bioassay is described for the study of inhibitory activity of plant proteins on fungal growth. Fungal spores were germinated in liquid growth medium and pipetted into wells of a microtitre plate. Fungal growth was followed spectrophotometrically. The bioassay was tested using crude protein extracts from plant tissues known to have high activities of chitinase and beta-1,3-glucanase, and with purified enzymes. Crude protein preparations and combinations of the purified enzymes produced a temporary reduction of growth but no permanent growth inhibition.
Ludwig
A
A
Botanisches Institut der Universität Basel, Switzerland.
Boller
T
T
D005658:Fungi / Q000254:growth & development* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests; D010940:Plant Proteins / Q000187:drug effects / Q000494:pharmacology*; D013057:Spectrum Analysis; D013172:Spores, Fungal; D013696:Temperature
D010940:Plant Proteins