[A simplified method for extracting and purifying bone morphogenetic protein].
Porcine bone morphogenetic protein was extracted and purified with general centrifugations and other biochemical techniques. Results showed that our methods were correct and reliable, which may cut down the costs of preparing BMP, simplify the procedure and improve the efficiency. Special polymerism by mesenchymal cells was observed during the early stage of inductive osteogenesis. The authors suggested this phenomenon probably be a sign which stood for the success of bone induction and a mark which the quality of inductive materials could be evaluated by.
Hu
X B
XB
People's Hospital of Hainan Province.
D000818:Animals; D019485:Bone Morphogenetic Proteins; D008722:Methods; D011506:Proteins / Q000302:isolation & purification*; D013552:Swine
D019485:Bone Morphogenetic Proteins; D011506:Proteins