A novel mast cell growth factor (MCGF-3) produced by marrow-adherent cells that synergizes with interleukin 3 and interleukin 4.
A clonal marrow-adherent stromal cell line, +/+-1 LDA11, was derived and found to produce hemopoietic stimulatory activity for an interleukin 3 (IL-3)-dependent mast cell line, NFS/N1. This factor-dependent mast cell line displayed restricted growth factor responsiveness to only IL-3, interleukin 4 (IL-4), and the stromal cell-produced factor. The factor produced by stromal cells was distinguished from IL-3 and IL-4 and was characterized biochemically. This factor appears to be a novel mast cell growth factor (MCGF-3) capable of synergizing with IL-3 and IL-4. It may have broader reactivity in hemopoiesis than simply IL-3-dependent mast cells, and it may prove relevant to stromal cell-mediated hemopoiesis.
Boswell
H S
HS
Hematology/Oncology Division, Veterans Administration Medical Center, Indianapolis, Indiana.
Mochizuki
D Y
DY
Burgess
G S
GS
Gillis
S
S
Walker
E B
EB
Anderson
D
D
Williams
D E
DE
D000818:Animals; D001853:Bone Marrow / Q000502:physiology*; D001854:Bone Marrow Cells; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line; D004357:Drug Synergism; D006133:Growth Substances / Q000494:pharmacology*; D006410:Hematopoiesis; D007377:Interleukin-3 / Q000494:pharmacology*; D015847:Interleukin-4 / Q000494:pharmacology*; D008407:Mast Cells / Q000166:cytology*; D051379:Mice
D006133:Growth Substances; D007377:Interleukin-3; D015847:Interleukin-4