Hypermetabolic priming of canine neutrophils by 7-S nerve growth factor.
Canine circulating neutrophils, isolated by a blood lysing technique, were incubated with 7-S nerve growth factor (NGF), at final concentrations between 12.5 and 800 ng/ml, for 30 minutes at 37 C. Neutrophil cytosolic H2O2 production, measured by flow cytometry, after 7-S NGF incubation was not significantly different from that produced at 37 C (baseline temperature controls) alone. Phorbol myristate acetate (PMA; 100 ng/ml) stimulation of neutrophils produced cytosolic H2O2 concentrations almost 13 times that of baseline temperature control neutrophils. Preincubation of neutrophils with 7-S NGF (100 to 800 ng/ml, 30 minutes, 37 C) and subsequent stimulation by PMA resulted in augmented H2O2 production in excess of twice that of neutrophils treated with PMA alone, and almost 30 times that of baseline temperature controls.
Gruber
D F
DF
Experimental Hematology Department, Armed Forces Radiobiology Research Institute, Bethesda, MD 20814-5145.
O'Halloran
K P
KP
D'Alesandro
M M
MM
Farese
A M
AM
D000818:Animals; D002469:Cell Separation; D004285:Dogs; D005434:Flow Cytometry; D006861:Hydrogen Peroxide / Q000378:metabolism*; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009504:Neutrophils / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000378:metabolism; D013997:Time Factors; D015854:Up-Regulation
D009414:Nerve Growth Factors; D006861:Hydrogen Peroxide; D013755:Tetradecanoylphorbol Acetate