[Isolation of tyrosyl-tRNA-synthetase from Thermus thermophilus HB-27].
A method for isolating tyrosyl-tRNA synthetase from Thermus thermophilus is described, including ammonium sulfate fractionation, chromatography on DEAE-sepharose, hydroxyapatite, heparin-sepharose and hydrophobic chromatography on Toyopearl HW-65. The yield of the purified enzyme was 1.6 mg per 1 kg of T. thermophilus cells. The enzyme is a dimer protein of the alpha 2 type with molecular weight of 100 kDa.
Iaremchuk
A D
AD
Tukalo
M A
MA
Egorova
S P
SP
Konovalenko
A V
AV
Matsuka
G Kh
GKh
D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification*; D002384:Catalysis; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D013696:Temperature; D013824:Thermus / Q000201:enzymology*; D014448:Tyrosine-tRNA Ligase / Q000302:isolation & purification*
D000604:Amino Acyl-tRNA Synthetases; D014448:Tyrosine-tRNA Ligase
Vydelenie tirozil-tRNK-sintetazy iz Thermus thermophilus HB-27.