Photo-oxidative damage to lenticular GAPDH and its relationship to aldehyde metabolism.
These studies show that GAPDH from the calf lens and human lens epithelium undergoes size and charge modifications in the presence of active oxygen species generated from methylene blue and light. The histidine content of the enzyme was reduced by one third. No evidence of increased proteolysis was observed suggesting a nonenzymatic cleavage of enzyme by the photo-oxidants.
Jedziniak
J
J
Harvard Medical School, Massachusetts Eye and Ear Infirmary, Howe Laboratory of Ophthalmology, Boston 02114.
D000447:Aldehydes / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000302:isolation & purification / Q000378:metabolism* / Q000528:radiation effects; D006801:Humans; D007908:Lens, Crystalline / Q000201:enzymology*; D008751:Methylene Blue; D010083:Oxidants, Photochemical; D010782:Photolysis
D000447:Aldehydes; D010083:Oxidants, Photochemical; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D008751:Methylene Blue