[The detection of lipofuscin in a culture of hybridoma cells].
Lipofuscin granules (LG) are found in the cultured hybridoma cells producing monoclonal antibodies to phage. LG have been studied using light and electron microscopy. Luminescent spectra of LG clusters in hybridoma cells are presented. The increase of own luminescence intensity of LG in the course of excitation by ultraviolet (365/nm) is shown. The advantages of hybridoma cells culture for investigation of LG on the cell level are discussed.
Tatariunas
A B
AB
Popov
V I
VI
Mantsygin
Iu A
IuA
Karnaukhov
V N
VN
D000818:Animals; D006825:Hybridomas / Q000032:analysis* / Q000648:ultrastructure; D008062:Lipofuscin / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D015591:Microspectrophotometry; D010860:Pigments, Biological / Q000032:analysis*
D008062:Lipofuscin; D010860:Pigments, Biological
Obnaruzhenie lipofustsina v kul'ture gibridomnykh kletok.