title: Transcription of a mutant collagen I gene is a cell type and stage-specific marker for odontoblast and osteoblast differentiation.
abstract: The Mov13 allele of the mouse alpha 1(I) collagen gene carries a retroviral insert in its first intron and had been reported to be transcriptionally silent. We have recently shown, however, that this mutant gene is expressed in odontoblasts of transplanted teeth derived from homozygous and heterozygous carrier embryos. The expression of the Mov13 allele has now been followed throughout in vivo development of mandibular teeth and bone in heterozygous animals, by in situ hybridization with a probe that specifically recognizes transcripts of the mutant gene. We show that the onset of its transcription precisely coincides with the final differentiation of odontoblasts and the onset of dentinogenesis, i.e. on day E16 for the incisor and at birth for the first molar. The mutant allele is also transcribed in osteoblasts of mandibular bone, again starting precisely with the onset of osteogenesis (day E13/14). No other cells were seen to transcribe the mutant gene. By these criteria, transcription of the Mov13 allele constitutes a true differentiation marker for odontoblasts and osteoblasts. Expression of the mutant allele in these two specialized cell types, in contrast to its transcriptional block in all other mesodermal cells ('fibroblasts'), suggests tissue-specific differences in the regulation of the alpha 1(I) collagen gene.
authors.affiliation: Institute of Molecular Biology, Austrian Academy of Sciences, Salzburg.
authors.forename: M
authors.initials: M
authors.lastname: Schwarz
authors.forename: K
authors.initials: K
authors.lastname: Harbers
authors.forename: K
authors.initials: K
authors.lastname: Kratochwil
mesh_terms: D000483:Alleles; D000818:Animals; D002454:Cell Differentiation; D003094:Collagen / Q000096:biosynthesis / Q000235:genetics*; D005819:Genetic Markers; D008334:Mandible / Q000166:cytology / Q000196:embryology; D051379:Mice; D008979:Moloney murine leukemia virus / Q000235:genetics; D009154:Mutation; D009693:Nucleic Acid Hybridization; D009804:Odontoblasts / Q000166:cytology*; D010006:Osteoblasts / Q000166:cytology*; D011533:Proviruses / Q000235:genetics; D015347:RNA Probes; D015183:Restriction Mapping; D014070:Tooth / Q000166:cytology / Q000196:embryology; D014158:Transcription, Genetic
chemical_list: D005819:Genetic Markers; D015347:RNA Probes; D003094:Collagen