Lipid profile of rat myelin subfractions.
Myelin from adult rat brains was separated on a discontinuous sucrose gradient into three subfractions. Analysis of "light", "heavy" and "membrane fraction" lipid classes was performed by HPTLC and densitometry while fatty acid composition was determinated by GLC. The more interesting results observed are: i) the "membrane fraction" resembles in its lipid and fatty acid composition other cell membranes (particularly oligodentrocytes); ii) "light" and "heavy" myelin are quite similar between them but the former has a higher content of sphingomyelin, a lower hydroxy/nonhydroxy cerebrosides ratio and a lower content of monoenoic fatty acids than the "heavy" subfraction. The results obtained could explain the different structures observed in each myelin subfraction since fatty acid composition, hydroxy fatty acids, sphingomyelin and cholesterol play a key role in the stability and structure of membranes.
Di Biase
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A
Department of Metabolism and Pathological Biochemistry, Istituto Superiore di Sanità, Rome, Italy.
Salvati
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S
Serlupi Crescenzi
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G
D000818:Animals; D001923:Brain Chemistry; D002784:Cholesterol / Q000032:analysis; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D003720:Densitometry; D005227:Fatty Acids / Q000032:analysis; D008055:Lipids / Q000032:analysis*; D008297:Male; D009186:Myelin Sheath / Q000032:analysis*; D019301:Oleic Acid; D009829:Oleic Acids / Q000032:analysis; D051381:Rats; D011917:Rats, Inbred Lew; D013109:Sphingomyelins / Q000032:analysis
D005227:Fatty Acids; D008055:Lipids; D009829:Oleic Acids; D013109:Sphingomyelins; D019301:Oleic Acid; D002784:Cholesterol