Proline uptake in Streptomyces clavuligerus.
Streptomyces clavuligerus was able to accumulate proline intracellularly throughout a wide range of external proline concentrations. Kinetic analysis of proline uptake indicated that this phenomenon is mediated by two saturable systems. One of them was a high-affinity system (Km = 11 microM), with low uptake capacity and specific for proline. The second system had lower affinity for proline (Km = 10.2 mM), higher uptake capacity, and was inhibited by several amino acids. Proline was not required as an inducer of the systems, which were neither repressed nor inhibited by ammonium.
Bascarán
V
V
Laboratorio de Microbiología, Departamento de Biología Funcional, Facultad de Medicina, Universidad de Oviedo, Spain.
Hardisson
C
C
Braña
A F
AF
D007700:Kinetics; D009584:Nitrogen / Q000494:pharmacology; D011392:Proline / Q000378:metabolism*; D013302:Streptomyces / Q000378:metabolism*
D011392:Proline; D009584:Nitrogen