DNA-sequence and metal-ion specificity of the formation of *H-DNA.
The homopyrimidine-homopurine sequence d(CT/GA)22 undergoes, in the presence of zinc ions, transition to an altered DNA conformation (*H-DNA) which is neither H-DNA nor B-DNA. *H-DNA is characterized by a peculiar chemical reactivity pattern in which most of the polypyrimidine strand is hyperreactive to osmium tetroxide and the central part of the polypurine strand is sensitive to diethylpyrocarbonate. Formation of *H-DNA is specific of metal-ion. *H-DNA is detected in the presence of Zn++, Cd++ and Mn++. The efficiency on promoting the transition is in the order of Zn++ greater than Cd++ much greater than Mn++. Formation of *H-DNA is also specific of nucleotide sequence. From all the different homopolymeric sequences tested only the d(CT/GA)22 sequence showed the zinc-induced transition to *H-DNA. These results suggest that stabilization of *H-DNA involves the formation of a specific complex between the metal-ion and the nucleotide sequence. The biological relevance of these results is discussed in view of the important role that zinc ions play on many nucleic acids processes.
Bernués
J
J
Grupo de Química Macromolecular, Centro de Investigación y Desarrollo CSIC, ETSEIB, Barcelona, Spain.
Beltrán
R
R
Casasnovas
J M
JM
Azorín
F
F
D001483:Base Sequence; D002104:Cadmium; D002118:Calcium; D003035:Cobalt; D003300:Copper; D004247:DNA; D008274:Magnesium; D008345:Manganese; D008969:Molecular Sequence Data; D009532:Nickel; D009690:Nucleic Acid Conformation; D015032:Zinc
D002104:Cadmium; D003035:Cobalt; D008345:Manganese; D003300:Copper; D009532:Nickel; D004247:DNA; D008274:Magnesium; D015032:Zinc; D002118:Calcium