[Conditioned medium of human nasopharyngeal carcinoma epithelioid cell line CNE 1 contained the activities of transformed growth-inhibiting factor(s)].
The hormone defined serum free conditioned medium (SFCM) of human nasopharyngeal carcinoma epithelioid cell line (CNE 1) was assaied by both the [3H] thymidine incorporation test and the soft agar test. It was found that the SFCM can stimulate the growth of long-term serum free cultured CNE 1 cells in accordence with the fact that the growth rate of long-term serum free cultured CNE 1 cells was directly proportional to the plating density. Alternatively 5% SFCM can inhibit the growth of short-term serum free cultured CNE 1 cells by 51% in which the indicator cell may remained the responsive state of growing in the serum supplemented medium to the effector of interest. Furthermore, SFCM resulted in inhibition of anchorage-independent growth of CNE 1 cells and A431 cells. Also in soft agar test, SFCM can reduce the colony formation of NRK-49F cells in the presence of EGF or EGF plus TGF-beta. These finding suggested that CNE 1 can autocrine growth stimulating factor(s) and growth inhibiting factor(s) in the serum free medium, the latter can strongly reverse malignant phenotypies of CNE 1 and A431 cells in serum supplemented surrounding.
Lu
Y L
YL
Shanghai Institute of Cell Biology, Academia Sinica.
Xu
Y H
YH
D001685:Biological Factors / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D003470:Culture Media; D004273:DNA, Neoplasm / Q000096:biosynthesis*; D004848:Epithelium / Q000378:metabolism; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000378:metabolism
D001685:Biological Factors; D003470:Culture Media; D004273:DNA, Neoplasm