title: Generation of a full-length human perinatal myosin heavy-chain-encoding cDNA.
abstract: Vertebrate sarcomeric myosin heavy chains (MHC) are encoded by multigene families whose members show tissue-specific and developmentally-regulated patterns of expression. Molecular genetic studies have allowed the cloning of a small number of complete genes or cDNAs encoding MHC isoforms [see Warrick and Spudich, Annu. Rev. Cell Biol. 3 (1987) 379-421]. Reported here is the isolation and sequence of a 2.6-kb cDNA that encodes the subfragment 1 or head of a human perinatal skeletal MHC. A cDNA sequence encoding the rod portion of this isoform has been previously reported [Feghali and Leinwand, J. Cell Biol. 108 (1989) 1791-1797]. Polymerase chain reaction with fetal skeletal muscle RNA was used to join the two nonoverlapping cDNA sequences to construct a full-length sequence. The gene encoding the perinatal skeletal MHC has been localized to the cluster of skeletal MHC-encoding genes on chromosome 17.
authors.affiliation: Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461.
authors.forename: I
authors.initials: I
authors.lastname: Karsch-Mizrachi
authors.forename: R
authors.initials: R
authors.lastname: Feghali
authors.forename: T B
authors.initials: TB
authors.lastname: Shows
authors.forename: L A
authors.initials: LA
authors.lastname: Leinwand
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D005333:Fetus; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D009132:Muscles / Q000378:metabolism; D015879:Myosin Subfragments / Q000235:genetics*; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D014178:Translocation, Genetic
chemical_list: D015879:Myosin Subfragments; D015345:Oligonucleotide Probes; D004247:DNA