Mitogenic effect of prolactin on chicken lymphocytes in vitro.
Lymphocytes obtained from thymus and spleens of 1-6 week old White Leghorn cockerels, untreated or immunized twice with sheep red blood cells (SRBC), were cultured with common T-cell mitogens in serial dilutions and/or different concentrations of bovine prolactin (PRL). [3H]Thymidine incorporation in newly synthesized DNA was used as a measure of lymphocyte mitogenic stimulation. Lymphocytes were stimulated by mitogens, as well as by PRL alone, in a dose-dependent way. Cell cultures prepared from immunized and non-immunized donors differed in their response to mitogens or PRL. The present results demonstrate direct PRL action on avian lymphoid cells and resemble those found in the mammalian immune system.
Skwarło-Sońta
K
K
Department of Vertebrate Animal Physiology, University of Warsaw, Poland.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002645:Chickens; D004261:DNA Replication / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D007114:Immunization; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D008934:Mitogens / Q000494:pharmacology; D008938:Mitosis / Q000187:drug effects*; D011388:Prolactin / Q000494:pharmacology*; D013045:Species Specificity
D008934:Mitogens; D011388:Prolactin