Effects of adenosine on inositol 1,4,5-trisphosphate formation and intracellular calcium changes in formyl-Met-Leu-Phe-stimulated human neutrophils.
In the presence of adenosine, formyl-Met-Leu-Phe-stimulated human neutrophils show a greatly diminished production of superoxide anion. Analysis of changes in levels of intracellular calcium revealed that the immediate increase (occurring within seconds) in intracellular calcium following addition of formyl-Met-Leu-Phe is not affected by the presence of adenosine, although there are significantly lower intracellular calcium levels during the late phase (occurring 1-4 min after addition of formyl-Met-Leu-Phe). Consistent with these findings is the fact that adenosine does not affect the production of inositol 1,4,5-triphosphate in formyl-Met-Leu-Phe-stimulated neutrophils. These data suggest that the profound inhibitory effects of adenosine on superoxide responses in formyl-Met-Leu-Phe-stimulated neutrophils may be related to an action of adenosine occurring late in the sequence of events of signal transduction.
Walker
B A
BA
Department of Pathology, University of Michigan Medical School, Ann Arbor 48109-0602.
Hagenlocker
B E
BE
Douglas
V K
VK
Ward
P A
PA
D000241:Adenosine / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*
D009240:N-Formylmethionine Leucyl-Phenylalanine; D015544:Inositol 1,4,5-Trisphosphate; D000241:Adenosine; D002118:Calcium