Shorter size of transmembrane glycoprotein of an HIV-1 isolate.
An HIV-1 strain carrying a shorter form of the transmembrane glycoprotein (TM) with a mobility of 32 kD, named KB-1, was isolated from a Japanese male hemophiliac by coculture of his peripheral blood mononuclear cells (PBMCs) with MT-2 cells and adaption to TALL-1 cells. Another HIV-1 strain, named KB-2, was isolated from his seropositive spouse by coculture of her PBMCs with MT-2 cells. The KB-2 strain carried a TM of ordinary size, with a mobility of 41 kD. The KB-1 strain carrying a truncated form of the TM could replicate in MT-2, MT-4, TALL-1 and MOLT-4 cells. The KB-1 strain is a useful HIV-1 isolate for investigating the function of the cytoplasmic domain of the TM and the significance of the presence of an in-frame stop codon in HIV env gene.
Shimizu
H
H
Division of AIDS Virus, National Institute of Health, Tokyo, Japan.
Morikawa
S
S
Yamaguchi
K
K
Tsuchie
H
H
Hachimori
K
K
Ushijima
H
H
Kitamura
T
T
D015153:Blotting, Western; D005260:Female; D006023:Glycoproteins / Q000032:analysis*; D015700:HIV Envelope Protein gp41 / Q000032:analysis*; D015497:HIV-1 / Q000302:isolation & purification / Q000378:metabolism*; D006467:Hemophilia A / Q000150:complications / Q000382:microbiology; D006801:Humans; D008297:Male; D008970:Molecular Weight; D014759:Viral Envelope Proteins / Q000032:analysis*
D006023:Glycoproteins; D015700:HIV Envelope Protein gp41; D014759:Viral Envelope Proteins