Isolation of a genomic clone encoding the rat histone variant, H1d.
Mammals contain a family of five closely related H1 histone variants (H1a-e) as well as two less closely related forms, H10 and H1t. We have sequenced a rat genomic clone that encodes one of the standard H1 variants. An RNA transcript of the gene was made with bacteriophage SP6 RNA polymerase and translated in a cell-free system. The protein synthesized in vitro was identified as variant H1d by its electrophoretic mobility.
Cole
K D
KD
Department of Chemistry, University of South Carolina, Columbia 29208.
Kandala
J C
JC
Kremer
E
E
Kistler
W S
WS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003001:Cloning, Molecular; D005796:Genes; D014644:Genetic Variation; D006657:Histones / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D051381:Rats; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014337:Trout
D006657:Histones