Implantation potential and fetal viability of mouse embryos cultured in media supplemented with platelet-activating factor.
Supplementation of culture media with PAF failed to increase the proportion of 2-cell mouse embryos which developed through to the expanded blastocyst stage after 72 h of culture in vitro. Culture of embryos with 1.86 microM-PAF, however, significantly increased the number of cells in expanded blastocysts. Culture in vitro with 0.186 or 1.86 microM-PAF significantly increased the ability of embryos to implant upon transfer to pseudopregnant recipients. The specificity of this effect was demonstrated by its inhibition by the specific PAF-receptor antagonist, SRI 63-441. Of the embryos which implanted, there was no difference in the proportions of PAF-treated or control embryos which resorbed nor was there any effect of PAF on fetal weight or crown-rump length measurements. Embryos cultured with 1.86 microM-PAF did have slightly heavier placentae than their respective controls. These results demonstrate the ability of exogenous PAF to enhance the viability of preimplantation mouse embryos cultured in vitro and support the hypothesis that a major site of the action of PAF during early pregnancy is the embryo itself.
Ryan
J P
JP
Human Reproduction Unit, Royal North Shore Hospital of Sydney, St Leonards, NSW, Australia.
Spinks
N R
NR
O'Neill
C
C
Wales
R G
RG
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D010064:Embryo Implantation; D004622:Embryo, Mammalian; D005314:Embryonic and Fetal Development / Q000187:drug effects; D005260:Female; D005328:Fetal Viability / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D010972:Platelet Activating Factor / Q000494:pharmacology / Q000502:physiology*
D003470:Culture Media; D010972:Platelet Activating Factor