Fractionation of macromolecules in an alternating transverse electric field: simulation of the method.
An electric field of alternating polarity applied in a direction transverse to the direction of solute transport is used as the basis of a method for the separation of biological macromolecules. The method derives directly from the ability of an electric field to induce movement of a charged macromolecule and from the physics of laminar fluid flow; no adsorptive immobile phase component is involved. The method is simulated by computer for the case of solute molecules in a solvent flowing through a narrow chamber of rectangular cross section. A voltage differential of periodically reversed polarity generates an electric field orthogonal to the direction of solvent flow. Solute molecules repetitively traverse the solvent channel at rates determined by their electrophoretic mobility. During the transit across the channel, solute molecules are transported in the direction of solvent flow; at the channel wall, solvent velocity is negligible and solute transport is limited to that provided by transient diffusion into a mobile solvent zone. Molecules of different intrinsic electrophoretic mobility are separated. The computer model was used to illustrate the process and to demonstrate the 'tunability' of the method as a function of the oscillation frequency and voltage wave form. Because of this tunability, a single instrument can function as the equivalent of several different chromatographic systems. Because fractionation is effected by direct physicochemical phenomena rather than via interaction with chromatographic sites, variations in fractionation results arising from formation of polymers for gel electrophoresis, packing of chromatography columns, or deterioration of columns with use are avoided. This method may be of particular use for the purification of nucleic acid fragments and for the analysis of protein: nucleic acid interactions.
Stevens
F J
FJ
Biological and Medical Research Division, Argonne National Laboratory, IL 60439-4833.
D005591:Chemical Fractionation / Q000379:methods; D003198:Computer Simulation; D004247:DNA / Q000302:isolation & purification*; D004560:Electricity; D004586:Electrophoresis; D008962:Models, Theoretical; D011506:Proteins / Q000302:isolation & purification*
D011506:Proteins; D004247:DNA