title: Disulfide bridges of bovine spleen cathepsin B.
abstract: Bovine spleen cathepsin B contains 7 disulfide bridges. Cleavage of the enzyme with cyanogen bromide gives rise to a large and a small fragment. The former contains all disulfide bridges. Their arrangement was determined by analysis of amino-acid sequences and compositions of subfragments prepared by cleavage of the large cyanogen-bromide fragment with trypsin, chymotrypsin and the staphylococcal proteinase using specific methods for the detection of S-S-bonds. Disulfide bridges link together Cys14-Cys43, Cys26-Cys71, Cys62-Cys128, Cys63-Cys67, Cys100-Cys132, Cys108-Cys119 and Cys148-Cys252.
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Prague, CSFR.
authors.forename: M
authors.initials: M
authors.lastname: Baudys
authors.forename: B
authors.initials: B
authors.lastname: Meloun
authors.forename: T
authors.initials: T
authors.lastname: Gan-Erdene
authors.forename: J
authors.initials: J
authors.lastname: Pohl
authors.forename: V
authors.initials: V
authors.lastname: Kostka
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002401:Cathepsin B / Q000032:analysis*; D002417:Cattle; D002850:Chromatography, Gel; D003432:Cross-Linking Reagents / Q000032:analysis*; D003545:Cysteine / Q000032:analysis; D004220:Disulfides / Q000032:analysis*; D008969:Molecular Sequence Data; D013154:Spleen / Q000201:enzymology*
chemical_list: D003432:Cross-Linking Reagents; D004220:Disulfides; D002401:Cathepsin B; D003545:Cysteine