[Comparison of organic dentin matrix composition between bovine permanent and deciduous teeth].
Minor differences in chemical composition of permanent and deciduous teeth have been suggested, however, clear data on the difference in organic composition have not yet been established. In the present study, organic dentin matrix components of bovine permanent and deciduous teeth were investigated. Bovine permanent and deciduous dentin were obtained from respective incisors and divided into crown and root. They were crushed into powder and extracted with 4M guanidine-HCl (G-extract), and then with 0.5 M EDTA, 4M guanidine-HCl (E-extract). E-extracts from permanent crown and root dentin and also deciduous crown and root dentin were separately applied to DEAE-cellulose column and each peak was examined precisely by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). A quantitative difference in organic matrix composition was recognized between permanent and deciduous dentin. Furthermore, a non-collagenous protein fraction, which existed in permanent teeth but not in deciduous ones, was discovered. This protein was purified as a single band in SDS-PAGE, which demonstrated an apparent molecular weight of approximately 30 Kd. Its amino acid composition was analyzed and the nature of this protein was discussed.
Nagai
H
H
Department of Pedodontics, Faculty of Dentistry, Tokyo Medical and Dental University.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D003804:Dentin / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D010750:Phosphoproteins / Q000032:analysis; D014070:Tooth / Q000032:analysis*; D014094:Tooth, Deciduous / Q000032:analysis*
D000596:Amino Acids; D010750:Phosphoproteins; C017630:phosphophoryn