Molecular size characterization of bacterial capsular polysaccharide vaccines by high performance liquid chromatography.
Bacterial capsular polysaccharides are major virulence factors and some are used as vaccinal antigens. Their molecular size is an important physicochemical criterion which correlates with immunogenicity. This article describes a new application of high performance liquid chromatography (HPLC), based on molecular sieving, for such an evaluation. This HPLC method is rapid, accurate, reproducible, requires only very low amounts of product and presents good correlation with conventional gel permeation chromatography.
Bussat
B
B
Research Department, Institut Mérieux, Marcy l'Etoile, France.
Schulz
D
D
Arminjon
F
F
Valentin
C
C
Armand
J
J
D016667:Bacterial Capsules; D001428:Bacterial Vaccines / Q000302:isolation & purification*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005069:Evaluation Studies as Topic; D018073:Haemophilus Vaccines; D006193:Haemophilus influenzae / Q000032:analysis / Q000276:immunology; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification*; D013296:Streptococcus pneumoniae / Q000032:analysis / Q000276:immunology
D001428:Bacterial Vaccines; D018073:Haemophilus Vaccines; C044237:Haemophilus influenzae type b polysaccharide vaccine; D011135:Polysaccharides, Bacterial