title: Effect of neural activity on skeletal muscle phosphoproteins.
abstract: A number of phosphoproteins were found in the soluble fraction of rat skeletal muscle by two-dimensional polyacrylamide gel electrophoresis (PAGE). The pattern of some of these phosphoproteins differed in fast (extensor digitorum longus, EDL) or slow (soleus) muscles, was dependent on normal innervation, and was altered with denervation. In order to determine if the pattern was maintained by electrical activity or trophic factors, we compared the effect of electrical block by local neural application of tetrodotoxin with the effect of complete nerve section. Both methods produced similar alterations in phosphoproteins, indicating that the pattern is dependent on nerve activity, not trophic factors. Such phosphoproteins are possible mediators of neural activity on gene expression.
authors.affiliation: Department of Medicine, University of Sydney, Australia.
authors.forename: G A
authors.initials: GA
authors.lastname: Nicholson
authors.forename: S
authors.initials: S
authors.lastname: Hawkins
authors.forename: J G
authors.initials: JG
authors.lastname: McLeod
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D009121:Muscle Denervation; D009124:Muscle Proteins / Q000032:analysis / Q000502:physiology*; D009132:Muscles / Q000032:analysis / Q000294:innervation* / Q000502:physiology; D009431:Neural Conduction / Q000502:physiology*; D010750:Phosphoproteins / Q000032:analysis / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D009124:Muscle Proteins; D010750:Phosphoproteins