[The effect of preparations of Hirudo medicinalis leeches on DNA methylation in the rat liver].
The effect of the salivary gland secretion and dialysable part of the homogenate of the leeches Hirudo medicinalis on the methylation of DNA in the rat liver after the intraperitoneal injection and perfusion of isolated liver has been analysed. The maximum concentration of 5-methylcytosine is observed 1 h later the injection of preparations: for the salivary gland secretion the increase is 39%, for the dialysate of leech homogenate is 28%. The 5-methylcytosine content increases on 28% after the perfusion of isolated liver with the leech saliva and after the dialysate of the leech homogenate--on 20%. No other changes in DNA content is observed. It is suggested that the DNA-methylation of the liver cells is due to the penetration of biologically active substances produced by the medical leech into the cell-targets accompanied by the forming of corresponding ligand-receptor complexes.
Nikonov
G I
GI
Romanenko
E B
EB
Vaniushin
B F
BF
Baskova
I P
IP
D044503:5-Methylcytosine; D000818:Animals; D003596:Cytosine / Q000031:analogs & derivatives / Q000032:analysis; D004247:DNA / Q000032:analysis / Q000187:drug effects* / Q000378:metabolism; D005215:Fasting; D007865:Leeches; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008745:Methylation; D051381:Rats; D012463:Saliva; D013997:Time Factors; D014020:Tissue Extracts / Q000494:pharmacology*
D014020:Tissue Extracts; D044503:5-Methylcytosine; D003596:Cytosine; D004247:DNA
Vliianie preparatov iz piiavok Hirudo medicinalis na metilirovanie DNK pecheni krys.