[Study of the in vivo penetration of cotrimoxazole in alveolar macrophages].
Kinetic of cotrimoxazole was studied in serum, alveolar macrophages and BAL fluid from guinea pigs receiving sulfamethoxazole (SMX, 100 mg/kg) and trimethoprim (TMP, 20 mg/kg). Guinea pigs were killed by cervical dislocation 30 min, 1 h, 3 h, 6 h and 24 h after intraperitoneal injection. Lung lavage was performed to obtain alveolar macrophages and BAL fluid. TMP and SMX levels were assayed using high-performance-liquid chromatography. Highest SMX levels were obtained in serum at 30 min, in BAL fluid at 1 h and in alveolar macrophages at 3 h. Mean SMX/TMP ratios (30 min, 1 h, 3 h) was 26.5 +/- 0.8 in serum, 3.76 +/- 1.8 in BAL fluid and 1.15 +/- 0.02 in alveolar macrophages.
Lopez
I
I
Laboratoire de Pathologie Respiratoire Expérimentale et de Pollution Atmosphérique, Institut Pasteur, Lille, France.
Dubar
V
V
Aerts
C
C
Voisin
C
C
Wallaert
B
B
D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics*; D001992:Bronchoalveolar Lavage Fluid / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004338:Drug Combinations / Q000032:analysis / Q000493:pharmacokinetics; D006168:Guinea Pigs; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D011650:Pulmonary Alveoli / Q000166:cytology* / Q000378:metabolism; D013420:Sulfamethoxazole / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics*; D014295:Trimethoprim / Q000032:analysis / Q000097:blood / Q000493:pharmacokinetics*
D000890:Anti-Infective Agents; D004338:Drug Combinations; D014295:Trimethoprim; D013420:Sulfamethoxazole
Etude de la pénétration in vivo du cotrimoxazole dans les macrophages alvéolaires.