title: [Degranulation of human eosinophilic granulocytes by the platelet activating factor].
abstract: Incubation of purified human eosinophil granulocytes (greater than 93%) with the platelet activating factor (PAF), led at concentrations of 1 pM to 10 microM, to a non-cytotoxic and dose-dependent liberation of proteins both from the "small" and the "specific" granules. The mean EC50 for this PAF-induced effect was 1.47 nM (n = 9), with a maximum at 100 nM PAF. The degranulating effect of PAF can be inhibited competitively by the specific PAF-receptor antagonist, WEB 2086 (KB between 12 and 13 nM), and is Ca2(+)-dependent.
authors.affiliation: Department of Thoracic Medicine, Brompton Hospital, University of London, United Kingdom.
authors.forename: C
authors.initials: C
authors.lastname: Kroegel
authors.forename: P
authors.initials: P
authors.lastname: Venge
authors.forename: H
authors.initials: H
authors.lastname: Matthys
authors.forename: P J
authors.initials: PJ
authors.lastname: Barnes
mesh_terms: D015550:Cell Degranulation / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004802:Eosinophilia / Q000276:immunology; D004804:Eosinophils / Q000187:drug effects*; D006801:Humans; D010972:Platelet Activating Factor / Q000494:pharmacology*
chemical_list: D010972:Platelet Activating Factor
vernacular_title: Degranulation humaner eosinophiler Granulozyten durch den Platelet Activating Factor (PAF).