title: Cultivation of sexually mature Brugia malayi in vitro.
abstract: Sexually mature male and female Brugia malayi were developed from third stage larvae after 60 days in the in vitro culture system described by Franke and others in 1987 (Am J Trop Med Hyg 37: 370-375). Between 75 and 100 days in culture, many worms produced living microfilariae. Each gravid female produced 200-1,500 microfilariae/day.
authors.affiliation: U.S. Naval Medical Research Unit No. 2, Jakarta Detachment, Indonesia.
authors.forename: W A
authors.initials: WA
authors.lastname: Riberu
authors.forename: S
authors.initials: S
authors.lastname: Atmosoedjono
authors.lastname: Purnomo
authors.forename: S
authors.initials: S
authors.lastname: Tirtokusumo
authors.forename: M J
authors.initials: MJ
authors.lastname: Bangs
authors.forename: J K
authors.initials: JK
authors.lastname: Baird
mesh_terms: D000818:Animals; D002009:Brugia / Q000254:growth & development* / Q000502:physiology; D003470:Culture Media; D005260:Female; D005298:Fertility; D006801:Humans; D008297:Male
chemical_list: D003470:Culture Media