A simple method for estimating association constants between monoclonal antibodies and derivatized or native antigens.
We describe a simple method for estimating the association constant between an antibody and an antigen, based on a theoretical treatment of experimental dose-response data. We used this method to calculate the association constants of an anti-progesterone monoclonal antibody for native progesterone and for progesterone 11 alpha-hemisuccinate. These association constants provide a quantitative measure of the bridging-group recognition by the antibody.
Schramm
W
W
University of Michigan, Ann Arbor 48109-2007.
Yang
T
T
Meyerhoff
M E
ME
Bachas
L G
LG
D000911:Antibodies, Monoclonal / Q000032:analysis*; D000936:Antigen-Antibody Complex / Q000032:analysis; D000941:Antigens / Q000032:analysis*; D001666:Binding Sites, Antibody; D003198:Computer Simulation; D008954:Models, Biological; D011374:Progesterone / Q000031:analogs & derivatives / Q000276:immunology
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000941:Antigens; D011374:Progesterone