Quantification of fluid changes in rat leg joints with adjuvant arthritis by a one-dimensional magnetic resonance imaging experiment.
A simple and reliable method for quantifying the fluid content and characterizing the tissue T2 relaxation properties of animal extremities has been devised. This one-dimensional magnetic resonance imaging experiment, applied to the adjuvant arthritis model in rats, provides a useful, noninvasive monitor of the course of the disease in vivo. Tissue is characterized through the analysis of the biexponential T2 decay of the NMR-active hydrogen in the rat leg joint tissue. Long and short T2 components are identified, both of which are sensitive to the arthritic process. The long component is suggested to come from edema and increases by a factor of ca. 9 during the course of the disease, whereas the short T2 component may represent cellular influx into the joint and increases by a factor of ca. 3. These changes correlate with the severity of the disease and can be used to monitor therapeutic response. The main advantage of the technique over the more traditional two-dimensional imaging approach is that only one spatial variable has to be resolved, resulting in shorter imaging time.
Borah
B
B
Norwich Eaton Pharmaceuticals, Inc., New York 13815.
Szeverenyi
N M
NM
D000818:Animals; D000843:Ankle Joint / Q000473:pathology*; D001168:Arthritis / Q000473:pathology*; D001169:Arthritis, Experimental / Q000473:pathology*; D002111:Calcaneus / Q000473:pathology; D005620:Freund's Adjuvant / Q000008:administration & dosage; D006859:Hydrogen; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008682:Metatarsal Bones / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D013582:Synovial Fluid / Q000032:analysis*; D013585:Synovitis / Q000473:pathology; D013643:Tarsus, Animal / Q000473:pathology; D013977:Tibia / Q000473:pathology
D006859:Hydrogen; D005620:Freund's Adjuvant