Measurement of lysergic acid diethylamide (LSD) in human plasma by gas chromatography/negative ion chemical ionization mass spectrometry.
A previously reported procedure for quantification of LSD in urine was modified to permit measurement of the drug in plasma. After addition of deuterium-labelled LSD, the plasma is extracted and the extract is treated with trifluoroacetylimidazole to convert the LSD to its N-trifluoroacetyl derivative. The derivatized LSD is analyzed by capillary column gas chromatography/negative ion chemical ionization. Plasma fortified with known concentrations of LSD gave linear responses from 0.1 to 3.0 ng/mL with this assay. The method was used to determine pharmacokinetic parameters for LSD after oral administration (1 microgram/kg) to a male volunteer. The apparent plasma half-life was determined to be 5.1 h. The peak plasma concentration of 1.9 ng/mL occurred 3 h after administration.
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Center for Human Toxicology, University of Utah, Salt Lake City 84108.
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D000284:Administration, Oral; D002138:Calibration; D002849:Chromatography, Gas / Q000379:methods; D006801:Humans; D008055:Lipids / Q000097:blood; D008238:Lysergic Acid Diethylamide / Q000008:administration & dosage / Q000097:blood*; D013058:Mass Spectrometry / Q000379:methods
D008055:Lipids; D008238:Lysergic Acid Diethylamide