Localization of an estrogen receptor binding site near the promoter of the uteroglobin gene.
By means of a DNA-cellulose competitive binding assay, we have studied the interaction of the estrogen receptor with genomic fragments of the estrogen responsive rabbit uteroglobin gene. The fragments spanned from 3255 bp upstream to 1754 bp downstream of the initiation site. Only a fragment (-396/+8) showed strong affinity for the receptor. Within this fragment a unique palindromic sequence (GGTCAccaTGCCC) was found which is very similar to the canonical consensus sequence for the estrogen receptor. A synthetic oligonucleotide of that structure specifically competed for the binding of the receptor to DNA-cellulose.
López de Haro
M S
MS
Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma, Canto Blanco, Madrid, Spain.
García
C
C
Nieto
A
A
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D001667:Binding, Competitive; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005260:Female; D005796:Genes; D006023:Glycoproteins / Q000235:genetics*; D007700:Kinetics; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D011401:Promoter Regions, Genetic; D011817:Rabbits; D011960:Receptors, Estrogen / Q000302:isolation & purification / Q000378:metabolism*; D015183:Restriction Mapping; D014598:Uteroglobin / Q000235:genetics*; D014599:Uterus / Q000378:metabolism*
D004268:DNA-Binding Proteins; D006023:Glycoproteins; D015345:Oligonucleotide Probes; D011960:Receptors, Estrogen; D004247:DNA; D014598:Uteroglobin