Effect of thimerosal in leukemia, in leukemic cell lines, and on normal hematopoiesis.
Anti-thymocyte globulin (ATG), a horse antiserum to human thymus tissue, has been shown to induce granulocytic differentiation of the HL-60 human leukemia cell line. In this paper we describe the effect of ATG on leukemic blasts and its effect on other human leukemia cell lines in vitro. The in vitro differentiation effect of ATG was observed in blasts from two patients with leukemia and the human leukemia cell line K562. The differentiation effect of ATG was attributable to its preservative, thimerosal, separable from ATG by high pressure liquid chromatography or dialysis. Subsequent studies with thimerosal alone showed it to induce differentiation in leukemic blasts from three patients and the human leukemia cell lines U937, K562, and KG-1. The differentiation effect of thimerosal is blocked by a sulfhydryl-protective agent, dithiothreitol, suggesting that the mechanism of differentiation may be mediated via a sulfhydryl group-dependent process.
Panella
T J
TJ
Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710.
Huang
A T
AT
D000961:Antilymphocyte Serum / Q000302:isolation & purification / Q000494:pharmacology*; D001752:Blast Crisis / Q000473:pathology; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D004261:DNA Replication / Q000187:drug effects; D005035:Ethylmercury Compounds / Q000494:pharmacology*; D006410:Hematopoiesis / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006454:Hemoglobins / Q000096:biosynthesis; D006801:Humans; D007938:Leukemia / Q000473:pathology; D013849:Thimerosal / Q000302:isolation & purification / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000166:cytology* / Q000187:drug effects
D000961:Antilymphocyte Serum; D005035:Ethylmercury Compounds; D006454:Hemoglobins; D013849:Thimerosal; D013936:Thymidine