Experimental glycerol myopathy: a histological study.
Histopathological changes induced by the intramuscular injection of glycerol were studied in the muscle fibers of rabbits. Fifteen minutes after the injection of 1 ml of 50% (v/v) glycerol, hypercontraction of fibers, disruption of the plasma membrane, and invasion of lanthanum into the sarcoplasm were observed. Between 12 and 24 h after the injection, more extensive pathological changes were seen which included: variation in fiber size; degeneration and necrosis of muscle fibers; hypercontraction of fibers by light microscopy; disruption of the plasma membrane by electron microscopy; vacuolar changes; hypercontraction of myofibrils, and selective loss of Z-bands. Between 7 and 14 days after glycerol injection, extensive regenerative changes were seen. The degenerative changes were similar to those seen in muscle in Duchenne muscular dystrophy, suggesting that a similar mechanism may be involved in the two conditions, so that experimental glycerol myopathy could be a good model for pathophysiological studies on Duchenne muscular dystrophy.
Kawai
H
H
First Department of Internal Medicine, School of Medicine, University of Tokushima, Japan.
Nishino
H
H
Kusaka
K
K
Naruo
T
T
Tamaki
Y
Y
Iwasa
M
M
D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D002118:Calcium / Q000032:analysis; D005260:Female; D005990:Glycerol / Q000633:toxicity*; D008854:Microscopy, Electron; D009132:Muscles / Q000187:drug effects / Q000473:pathology* / Q000648:ultrastructure; D009135:Muscular Diseases / Q000139:chemically induced / Q000473:pathology* / Q000503:physiopathology; D009336:Necrosis; D011817:Rabbits; D012038:Regeneration
D000135:Acid Phosphatase; D005990:Glycerol; D002118:Calcium