Enhanced synthesis of heat shock proteins and augmented thermotolerance after induction of differentiation in HL-60 human leukemia cells.
The effects of the induction of differentiation were investigated on the expression of heat shock proteins (hsps) and thermotolerance. The synthesis of the major hsps in response to heat stress was markedly enhanced in HL-60 human leukemia cells after differentiation. An increased amount of mRNA transcripts for hsp 70 was also noted. In addition, induction of differentiation resulted in acquisition of greater resistance to heat, which may be advantageous since cells in the peripheral blood must survive many stresses.
Yufu
Y
Y
Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Nishimura
J
J
Ideguchi
H
H
Nawata
H
H
D015152:Blotting, Northern; D002454:Cell Differentiation; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006358:Hot Temperature; D006801:Humans; D066298:In Vitro Techniques; D015470:Leukemia, Myeloid, Acute; D008970:Molecular Weight; D012333:RNA, Messenger / Q000235:genetics; D014407:Tumor Cells, Cultured
D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D004121:Dimethyl Sulfoxide