The cholecystokinin gene is abundantly co-expressed with gastrin-releasing peptide, enkephalin and neuropeptide Y genes in a clonal human neuroepithelioma cell line.
The cholinergic human neuroepithelioma cell line SK-N-MCIXC expressed mRNAs for the neuropeptides cholecystokinin (CCK), neuropeptide Y, gastrin-releasing peptide (GRP) and enkephalin. The CCK transcript of about 800 nt was present at very high levels and CCK-like peptides immunoreactive to a C-terminal CCK octapeptide antiserum were present in the cell line and its medium. This clonal neuronal cell line provides a unique model system to identify cis- and trans-acting factors responsible for neuron-specific expression and regulation of the CCK gene. Furthermore, the pluripotent properties of the undifferentiated cell line may open studies on neuronal differentiation at the level of co-expression of neuropeptides and transmitters.
Verbeeck
M A
MA
Rudolf Magnus Institute, Medical Faculty, University of Utrecht, The Netherlands.
Burbach
J P
JP
D015152:Blotting, Northern; D002766:Cholecystokinin / Q000235:genetics*; D004745:Enkephalins / Q000235:genetics*; D019886:Gastrin-Releasing Peptide; D015870:Gene Expression; D006801:Humans; D018241:Neuroectodermal Tumors, Primitive, Peripheral / Q000235:genetics* / Q000473:pathology; D009478:Neuropeptide Y / Q000235:genetics*; D009479:Neuropeptides / Q000235:genetics; D010455:Peptides / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D014407:Tumor Cells, Cultured
D004745:Enkephalins; D009478:Neuropeptide Y; D009479:Neuropeptides; D010455:Peptides; D012333:RNA, Messenger; D019886:Gastrin-Releasing Peptide; D002766:Cholecystokinin