title: Aluminum-induced neo-osteogenesis: a generalized process affecting trabecular networking in the axial skeleton.
abstract: To determine if aluminum-induced neo-osteogenesis occurs in the axial skeleton, we compared spinal bone density and vertebral histology of beagles treated with aluminum for 8 and 16 weeks to that of untreated normals. Administration of aluminum (1.25 mg/kg) did not alter serum calcium, phosphorus, or creatinine but did result in a significant elevation of vertebral bone density, measured by quantitative computed tomography, after both 8 (286.7 +/- 12.4 mg/ml) and 16 (361.7 +/- 46.5 mg/ml) weeks of treatment compared with controls (212.2 +/- 4.5 mg/ml). In accord with the increased bone density, biopsies from the spine displayed evidence of neo-osteogenesis, including the presence of woven bone, both mineralized and unmineralized, within the marrow space. The genesis of such woven bone units resulted after 16 weeks in a significant increase in trabecular bone volume, woven and lamellar (51.2 +/- 4.4 versus 32.4 +/- 1.2%; p less than 0.05), woven bone volume (9.1 +/- 3.6 versus 0 +/- 0%; p less than 0.05), and trabecular number (4.5 +/- 0.3 versus 3.5 +/- 0.2 per mm; p less than 0.05). In addition, scanning electron microscopic evaluation of the bone biopsies confirmed the existence of new trabecular plates that provided interconnections between existent units. These observations illustrate that aluminum-induced neo-osteogenesis positively influences trabecular networking in the axial skeleton. Such enhancement of bone histogenesis contrasts with the effects of other pharmacologic agents that solely alter the thickness of existing trabecular plates or rods within the vertebral spongiosa.
authors.affiliation: Department of Medicine, Duke University Medical Center, Durham, NC.
authors.forename: L D
authors.initials: LD
authors.lastname: Quarles
authors.forename: G
authors.initials: G
authors.lastname: Murphy
authors.forename: J B
authors.initials: JB
authors.lastname: Vogler
authors.forename: M K
authors.initials: MK
authors.lastname: Drezner
mesh_terms: D000535:Aluminum / Q000633:toxicity*; D000818:Animals; D015519:Bone Density / Q000187:drug effects; D002118:Calcium / Q000097:blood; D003401:Creatine / Q000097:blood; D004285:Dogs; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis / Q000187:drug effects*; D010758:Phosphorus / Q000097:blood; D013131:Spine / Q000187:drug effects* / Q000473:pathology; D014057:Tomography, X-Ray Computed
chemical_list: D010758:Phosphorus; D000535:Aluminum; D003401:Creatine; D002118:Calcium