Aqueous humour analysis in Fuchs' heterochromic cyclitis.
Aqueous humor from 23 patients with Fuchs' heterochromic cyclitis (FHC) was analysed by a number of immunological methods. Intraocular IgG synthesis was found in 65% of patients and oligoclonal IgG bands, mainly of the IgG1 subclass, identified in 57%. There was a relative increase in IgG1 (P less than 0.01) as compared to patients with senile cataract. Local production of the cytokine Interleukin-6 was demonstrated in 63% of patients (P less than 0.01). Analysis of aqueous by HPLC and SDS-PAGE failed to reveal any abnormalities specific for FHC. These findings add further evidence to the theory of immune dysregulation in this condition.
Murray
P I
PI
Department Ophthalmo-Immunology, The Netherlands Ophthalmic Research Institute, Amsterdam.
Hoekzema
R
R
van Haren
M A
MA
Luyendijk
L
L
Kijlstra
A
A
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000418:Albumins / Q000378:metabolism; D001082:Aqueous Humor / Q000276:immunology / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000378:metabolism; D007132:Immunoglobulin Isotypes; D015850:Interleukin-6 / Q000378:metabolism; D015863:Iridocyclitis / Q000276:immunology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012709:Serum Albumin / Q000378:metabolism
D000418:Albumins; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D015850:Interleukin-6; D012709:Serum Albumin