The assignment of the genes coding for human complement components C6 and C7 to chromosome 5.
A panel of 19 somatic cell hybrids was tested for the presence of human sequences coding for complement components C6 and C7 by restriction enzyme digestion and Southern blots probed with human C6 and C7 cDNA probes. C7 was also detected by amplifying part of the human gene in hybrid DNA using the polymerase chain reaction. Detection of human C6 and C7 was completely correlated with the presence of chromosome 5.
Jeremiah
S J
SJ
MRC Human Biochemical Genetics Unit, Galton Laboratory, University College London.
Abbott
C M
CM
Murad
Z
Z
Povey
S
S
Thomas
H J
HJ
Solomon
E
E
DiScipio
R G
RG
Fey
G H
GH
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5; D003183:Complement C6 / Q000235:genetics*; D003184:Complement C7 / Q000235:genetics*; D006224:Cricetinae; D015342:DNA Probes; D005796:Genes; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D003183:Complement C6; D003184:Complement C7; D015342:DNA Probes