title: DNA damage induced by N-nitrosodibenzylamine and N-nitroso-alpha-acetoxybenzyl-benzylamine in mammalian cell systems and in vivo.
abstract: N-nitrosodibenzylamine (NDBzA) and N-nitroso-alpha-acetoxybenzyl-benzylamine (alpha-acetoxy-NDBzA) were tested for induction of DNA single-strand breaks (SSBs) in V 79 Chinese hamster cells (V 79 cells) and isolated rat hepatocytes. The alkaline elution assay was used for the detection of DNA strand breaks. Treatment of V 79 cells with alpha-acetoxy-NDBzA effectively increased the rate of DNA SSBs, while with NDBzA, no DNA-damaging activity was detectable. Both substances produced significant DNA damage in rat hepatocytes. Interestingly, NDBzA was able to induce SSBs at significantly lower concentrations than alpha-acetoxy-NDBzA. The possible reasons for these findings are discussed. In contrast to these in vitro results, NDBzA exhibited very weak in vivo activity.
authors.affiliation: Max von Pettenkofer Institute, Federal Health Office, Berlin, F.R.G.
authors.forename: K E
authors.initials: KE
authors.lastname: Appel
authors.forename: S
authors.initials: S
authors.lastname: Görsdorf
authors.forename: M
authors.initials: M
authors.lastname: Menden
authors.forename: M
authors.initials: M
authors.lastname: Lauterbach
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002460:Cell Line / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000032:analysis / Q000187:drug effects*; D005260:Female; D007274:Injections, Intraperitoneal; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D009602:Nitrosamines / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D004277:DNA, Single-Stranded; D009602:Nitrosamines; C015395:N-nitrosodibenzylamine; C019232:N-(alpha-acetoxybenzyl)-N-benzylnitrosamine