Improvement of radioactive colloid binding by tumor cells.
Treatment of P388 leukemia cells with poly-DL-lysine (Poly-lys) considerably increases the binding of colloidal chromic phosphate (32P). This augmentation of the number of particles that are bound is in direct relationship with Poly-lys concentration, and very significantly with its degree of polymerization. Treatment with Poly-lys molecular weight 77,000 shows a 100% increase of binding with 200 micrograms and of 50% with 100 micrograms. Poly-lys M.W. 8,000 presents no significant increase, and for the other molecular weights the binding is intermediate.
Anghileri
L J
LJ
Laboratory of Biophysics, Faculty of Medicine, University of Nancy, France.
Marchal
C
C
Maincent
P
P
Robert
J
J
D000327:Adsorption; D000818:Animals; D002857:Chromium / Q000378:metabolism; D017608:Chromium Compounds; D003102:Colloids; D007941:Leukemia P388 / Q000378:metabolism*; D007942:Leukemia, Experimental / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D010710:Phosphates / Q000378:metabolism; D010761:Phosphorus Radioisotopes / Q000378:metabolism*; D011107:Polylysine / Q000494:pharmacology*
D017608:Chromium Compounds; D003102:Colloids; D010710:Phosphates; D010761:Phosphorus Radioisotopes; D002857:Chromium; D011107:Polylysine; C509369:chromic phosphate