title: [Isolation of tyrosyl-tRNA-synthetase from Thermus thermophilus HB-27].
abstract: A method for isolating tyrosyl-tRNA synthetase from Thermus thermophilus is described, including ammonium sulfate fractionation, chromatography on DEAE-sepharose, hydroxyapatite, heparin-sepharose and hydrophobic chromatography on Toyopearl HW-65. The yield of the purified enzyme was 1.6 mg per 1 kg of T. thermophilus cells. The enzyme is a dimer protein of the alpha 2 type with molecular weight of 100 kDa.
authors.forename: A D
authors.initials: AD
authors.lastname: Iaremchuk
authors.forename: M A
authors.initials: MA
authors.lastname: Tukalo
authors.forename: S P
authors.initials: SP
authors.lastname: Egorova
authors.forename: A V
authors.initials: AV
authors.lastname: Konovalenko
authors.forename: G Kh
authors.initials: GKh
authors.lastname: Matsuka
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification*; D002384:Catalysis; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D013696:Temperature; D013824:Thermus / Q000201:enzymology*; D014448:Tyrosine-tRNA Ligase / Q000302:isolation & purification*
chemical_list: D000604:Amino Acyl-tRNA Synthetases; D014448:Tyrosine-tRNA Ligase
vernacular_title: Vydelenie tirozil-tRNK-sintetazy iz Thermus thermophilus HB-27.