title: Functional measurements on carboxymethylated hemoglobin, a potential blood substitute: effects of sodium chloride on pH values that influence the alkaline Bohr effect.
abstract: The leakage of chloride from electrodes during measurements of the alkaline Bohr effect of hemoglobin (by the proton release method) amounted to 1-5 mM concentration of the anion depending on the type of electrode employed. This concentration, together with the amount of chloride found to be intrinsically bound to hemoglobin (0.2-0.6 mM), could mask the contribution of chloride to various hemoglobin functions. In addition, the concentration of chloride was found to affect the pH of buffers as measured either with a pH meter or with the dye, cresol red. Thus, for 20 mM phosphate buffer, the pH was lowered almost 0.4 pH units in the presence of 0.30 M chloride. For Tris-acetate buffer, the same concentration of chloride led to an increase in pH of about 0.05 units.
authors.affiliation: Rockefeller University, New York, NY 10021.
authors.forename: L R
authors.initials: LR
authors.lastname: Manning
authors.forename: W J
authors.initials: WJ
authors.lastname: Fantl
authors.forename: J M
authors.initials: JM
authors.lastname: Manning
mesh_terms: D001802:Blood Substitutes; D002021:Buffers; D004566:Electrodes; D006441:Hemoglobin A; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D012965:Sodium Chloride
chemical_list: D001802:Blood Substitutes; D002021:Buffers; C064928:hemoglobin A, carboxymethylated-; D012965:Sodium Chloride; D006441:Hemoglobin A