DNA damage induced by N-nitrosodibenzylamine and N-nitroso-alpha-acetoxybenzyl-benzylamine in mammalian cell systems and in vivo.
N-nitrosodibenzylamine (NDBzA) and N-nitroso-alpha-acetoxybenzyl-benzylamine (alpha-acetoxy-NDBzA) were tested for induction of DNA single-strand breaks (SSBs) in V 79 Chinese hamster cells (V 79 cells) and isolated rat hepatocytes. The alkaline elution assay was used for the detection of DNA strand breaks. Treatment of V 79 cells with alpha-acetoxy-NDBzA effectively increased the rate of DNA SSBs, while with NDBzA, no DNA-damaging activity was detectable. Both substances produced significant DNA damage in rat hepatocytes. Interestingly, NDBzA was able to induce SSBs at significantly lower concentrations than alpha-acetoxy-NDBzA. The possible reasons for these findings are discussed. In contrast to these in vitro results, NDBzA exhibited very weak in vivo activity.
Appel
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Max von Pettenkofer Institute, Federal Health Office, Berlin, F.R.G.
Görsdorf
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S
Menden
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Lauterbach
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D000284:Administration, Oral; D000818:Animals; D002460:Cell Line / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage; D004277:DNA, Single-Stranded / Q000032:analysis / Q000187:drug effects*; D005260:Female; D007274:Injections, Intraperitoneal; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D009602:Nitrosamines / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains
D004277:DNA, Single-Stranded; D009602:Nitrosamines; C015395:N-nitrosodibenzylamine; C019232:N-(alpha-acetoxybenzyl)-N-benzylnitrosamine