title: In vitro evaluation of fibroplasia in a porous polymer.
abstract: We evaluated a polybutylene/polypropylene blend in a blown microfiber configuration in vitro for potential use as the peripheral area of a keratoprosthetic device. Material properties such as ultimate tensile strength and ultimate elongation were measured. Stromal fibroblasts were seeded onto the material in vitro, and cell proliferation between uncoated and Type I collagen coated discs did not differ significantly. Fibroblasts could be seen migrating along the fibers and also traversing the fibers. The synthesis of connective proteins was examined. Laminin, fibronectin, and Type I collagen were detected by day 8. The experiments demonstrate that stromal fibroblasts can adhere onto the fibers, proliferate, and synthesize connective tissue proteins. Experiments are now being conducted to further evaluate the material in vivo.
authors.affiliation: Boston University School of Medicine, School of Medicine, Ophthalmology Department, MA 02118.
authors.forename: V
authors.initials: V
authors.lastname: Trinkaus-Randall
authors.forename: J
authors.initials: J
authors.lastname: Capecchi
authors.forename: L
authors.initials: L
authors.lastname: Sammon
authors.forename: D
authors.initials: D
authors.lastname: Gibbons
authors.forename: H M
authors.initials: HM
authors.lastname: Leibowitz
authors.forename: C
authors.initials: C
authors.lastname: Franzblau
mesh_terms: D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D003319:Corneal Stroma / Q000166:cytology; D016039:Corneal Transplantation; D005347:Fibroblasts / Q000166:cytology* / Q000648:ultrastructure; D005353:Fibronectins / Q000096:biosynthesis; D066298:In Vitro Techniques; D007797:Laminin / Q000096:biosynthesis; D008422:Materials Testing; D011108:Polymers; D011126:Polypropylenes; D011817:Rabbits
chemical_list: D005353:Fibronectins; D007797:Laminin; D011108:Polymers; D011126:Polypropylenes; D003094:Collagen