Characterization of the negative regulatory element of the 5' noncoding region of Moloney murine leukemia virus in mouse embryonal carcinoma cells.
An intragenic untranslated region of Moloney murine leukemia virus suppressed gene expression from a long terminal repeat about 30-fold in F9 cells, an embryonal carcinoma (EC) cell line. In another EC line, PCC4 cells and their subline, EA2 cells, and in a fibroblast line, NIH3T3 cells, the suppression by this region was about 4- and 3-fold, respectively. Deletion analysis indicated that the upstream of the previously defined region is required for the suppression. Although not as efficient, this region exhibited suppressive activity even when placed upstream of the transcriptional start site of the long terminal repeat. These results suggest that the suppression is cell type specific and operates partly at the level of the initiation of transcription.
Tsukiyama
T
T
Department of Pathology, Hiroshima University, Japan.
Niwa
O
O
Yokoro
K
K
D000818:Animals; D002460:Cell Line; D002872:Chromosome Deletion; D005814:Genes, Viral; D007438:Introns; D051379:Mice; D008979:Moloney murine leukemia virus / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D013489:Suppression, Genetic; D013724:Teratoma