title: Growth responses of prostatic epithelial cells from male mice neonatally exposed to diethylstilbestrol in serum-free collagen gel culture.
abstract: Growth of anterior and ventral prostatic epithelial cells from mice neonatally exposed to diethylstilbestrol (DES) and from unexposed control mice was compared at different time points in serum-free collagen gel culture. A longer maintenance of the initial plating density (lag in growth) was observed in cultured DES-exposed ventral prostatic cells. Neonatal DES exposure resulted in two colony types: one similar to colonies arising from unexposed cells and one which appears to be non-growing. Keratinization was observed in some DES-exposed anterior prostatic cell colonies. Removal of epidermal growth factor from the serum-free medium significantly decreased growth in 3 of the 4 groups compared with their growth in the complete serum-free medium.
authors.affiliation: Cancer Research Laboratory, University of California, Berkeley 94720.
authors.forename: T
authors.initials: T
authors.lastname: Turner
authors.forename: H A
authors.initials: HA
authors.lastname: Bern
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002455:Cell Division / Q000187:drug effects; D003094:Collagen; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods; D004054:Diethylstilbestrol / Q000494:pharmacology*; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D007700:Kinetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011467:Prostate / Q000166:cytology* / Q000187:drug effects; D012016:Reference Values
chemical_list: D003470:Culture Media; D004054:Diethylstilbestrol; D003094:Collagen