The hemagglutinating glycoproteins of influenza B and C viruses are acylated with different fatty acids.
We present evidence that the hemagglutinin (HA) of influenza B virus and the glycoprotein of influenza C virus (HEF) are acylated. The fatty acid linkage is sensitive to treatment with hydroxylamine and mercaptoethanol, which points to a labile thioester-type linkage. The HA of influenza B virus contains mainly palmitic acid, whereas the HEF glycoprotein of influenza C virus is acylated with stearic acid which has not been observed before as the prevailing fatty acid in viral or cellular acyl proteins.
Veit
M
M
Institut für Virologie, Philipps-Universität Marburg, Federal Republic of Germany.
Herrler
G
G
Schmidt
M F
MF
Rott
R
R
Klenk
H D
HD
D000215:Acylation; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D005227:Fatty Acids / Q000032:analysis* / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral / Q000302:isolation & purification*; D019811:Hydroxylamine; D006898:Hydroxylamines; D009981:Influenza B virus / Q000032:analysis* / Q000378:metabolism; D009982:Gammainfluenzavirus / Q000032:analysis* / Q000378:metabolism; D008623:Mercaptoethanol; D019814:Myristic Acid; D009227:Myristic Acids / Q000032:analysis; D009975:Orthomyxoviridae / Q000032:analysis*; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000032:analysis; D013229:Stearic Acids / Q000032:analysis; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000302:isolation & purification*
D000596:Amino Acids; D005227:Fatty Acids; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral; D006898:Hydroxylamines; D009227:Myristic Acids; D010169:Palmitic Acids; D013229:Stearic Acids; D014759:Viral Envelope Proteins; D019814:Myristic Acid; D019811:Hydroxylamine; D019308:Palmitic Acid; C031183:stearic acid; D008623:Mercaptoethanol; D005944:Glucosamine