title: Molecular cloning of guinea-pig aromatic-L-amino acid decarboxylase cDNA.
abstract: Guinea-pig aromatic-L-amino acid decarboxylase (DOPA decarboxylase, DDC), but not rat DDC, reacts with the antibody against rat histidine decarboxylase (HDC). For determination of the molecular reaction for this cross-reactivity, a cDNA clone of guinea-pig DDC was isolated. Guinea-pig DDC consists of 480 amino acids and its molecular weight is 54,148. The sequence identify of guinea-pig DDC with rat DDC is 86%. Guinea-pig DDC has a region showing 100% sequence identity with rat HDC, but only 67% sequence identity with rat DDC, suggesting that this region is related with the cross-reactivity of guinea-pig DDC and anti-rat HDC antibody.
authors.affiliation: Department of Pharmacology II, Osaka University, Faculty of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Taketoshi
authors.forename: Y
authors.initials: Y
authors.lastname: Horio
authors.forename: I
authors.initials: I
authors.lastname: Imamura
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: H
authors.initials: H
authors.lastname: Fukui
authors.forename: H
authors.initials: H
authors.lastname: Wada
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003429:Cross Reactions; D004247:DNA / Q000235:genetics*; D004274:DNA, Recombinant / Q000032:analysis*; D004296:Dopa Decarboxylase / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D006168:Guinea Pigs; D006640:Histidine Decarboxylase / Q000032:analysis / Q000276:immunology; D008969:Molecular Sequence Data; D051381:Rats; D015183:Restriction Mapping
chemical_list: D004274:DNA, Recombinant; D004247:DNA; D004296:Dopa Decarboxylase; D006640:Histidine Decarboxylase; D001142:Aromatic-L-Amino-Acid Decarboxylases