Cloning of the human brain GABA transporter.
A cDNA clone encoding a transporter for the neurotransmitter gamma-aminobutyric acid in human brain was cloned and sequenced. The cDNA contains an open reading frame encoding a hydrophobic protein of 599 amino acids with a calculated molecular weight of 67022 Da. Hydropathy analysis revealed twelve potential transmembrane segments. The human protein is highly homologous to the protein from rat brain. Northern hybridization demonstrated a ubiquitous distribution of the transporter in various parts of the brain.
Nelson
H
H
Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110.
Mandiyan
S
S
Nelson
N
N
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000502:physiology; D002352:Carrier Proteins / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D050485:GABA Plasma Membrane Transport Proteins; D006801:Humans; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D027361:Organic Anion Transporters; D015183:Restriction Mapping; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D002352:Carrier Proteins; D050485:GABA Plasma Membrane Transport Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D027361:Organic Anion Transporters; D005680:gamma-Aminobutyric Acid; D004247:DNA