The survival of subgingival plaque bacteria in an amine fluoride-containing gel.
Subgingival plaque samples from 20 patients with chronic inflammatory periodontal disease were exposed to a commercial gel formulation containing 2 amine fluorides. The MIC of the gel for these samples ranged from 33 to 260 micrograms/ml with a modal value of 260 micrograms/ml. In each sample, the most resistant organisms (i.e., those organisms surviving at one doubling dilution below the MIC) were identified. 33 such organisms were isolated, of which 22 (67%) were strict anaerobes, and 25 (75%) were Gram negative. The 2 most frequent isolates were Bacteroides ruminicola ss. brevis and a Selenomonas species, neither of which is a recognised periodontopathogen. The gel had a rapid effect on the viability of the bacteria in the plaque samples, the 90% kill time being 17 min or less for 90% of the samples (range less than 5 to 71 min).
Bansal
G S
GS
Department of Clinical Pathology, Institute of Dental Surgery, London.
Newman
H N
HN
Wilson
M
M
D000328:Adult; D000588:Amines / Q000008:administration & dosage / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D001439:Bacteroides / Q000187:drug effects; D002908:Chronic Disease; D015169:Colony Count, Microbial; D003773:Dental Plaque / Q000382:microbiology*; D003959:Diamines; D004305:Dose-Response Relationship, Drug; D004352:Drug Resistance, Microbial; D005260:Female; D005459:Fluorides / Q000008:administration & dosage / Q000494:pharmacology*; D005782:Gels; D006801:Humans; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000382:microbiology*; D013997:Time Factors
D000588:Amines; D003959:Diamines; D005782:Gels; C495399:Olaflur; D005459:Fluorides