Forskolin modulates acetylcholine release in the hippocampus independently of adenylate cyclase activation.
[3H]Acetylcholine release from slices of rabbit hippocampus was elicited by electrical field stimulation (360 pulses/3 Hz). Both forskolin, commonly used as a specific activator of adenylate cyclase, as well as 1,9-dideoxy-forskolin, which fails to activate adenylate cyclase, increased the evoked transmitter release in an almost identical manner. In addition, the phosphodiesterase inhibitor, rolipram, and the membrane-permeable analogue of cAMP, 8-Br-cAMP, did not influence acetylcholine release. These data show that forskolin is not specific to adenylate cyclase and that the increase in acetylcholine release in the rabbit hippocampus occurs through a mechanism other than activation of adenylate cyclase.
Allgaier
C
C
Institut für Pharmakologie und Toxikologie, Universität Freiburg, F.R.G.
Choi
B K
BK
Hertting
G
G
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000109:Acetylcholine / Q000378:metabolism*; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D005576:Colforsin / Q000494:pharmacology*; D004558:Electric Stimulation; D004789:Enzyme Activation / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D066298:In Vitro Techniques; D011760:Pyrrolidinones / Q000494:pharmacology; D011817:Rabbits; D020889:Rolipram
D011760:Pyrrolidinones; D005576:Colforsin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D000262:Adenylyl Cyclases; D020889:Rolipram; D000109:Acetylcholine