The gene for human acidic fibroblast growth factor encodes two upstream exons alternatively spliced to the first coding exon.
We have isolated two cDNA clones encoding human acidic fibroblast growth factor (aFGF) which represent the utilization of alternative upstream exons in aFGF mRNA. Isolation and sequence analysis of genomic clones spanning the first coding exon and each of the upstream sequences confirms that the divergent 5' sequences are separate exons, spliced alternatively to the first coding exon 34 nucleotides upstream of the initiator AUG codon. Restriction mapping of the genomic clones provides a minimum size estimate of 45 kilobase pairs for the aFGF locus.
Crumley
G
G
Rorer Central Research, King of Prussia, PA 19406.
Dionne
C A
CA
Jaye
M
M
D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005091:Exons; D005346:Fibroblast Growth Factors / Q000235:genetics*; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D012326:RNA Splicing; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping
D005346:Fibroblast Growth Factors; D004247:DNA