Preparation of fetal rat brains for light and electron microscopy.
To study cellular shapes, growth patterns, and fine structure during early stages of CNS development in rat embryos, preparative procedures were evaluated and modified to meet two criteria: 1) Coronal semithin sections should reveal undeformed telencephalic hemispheres that were symmetrically expanded on both sides of midline structures and were surrounded by contiguous mesenchyme. 2) In electron micrographs, cells should have intact, undistorted surface membranes, evenly distributed nucleoplasm and well preserved cytoplasmic organelles. To meet these criteria, 378 fetuses with a gestational age of 11-20 days (E11-E20) were used to test and modify procedures for anesthesia, embryo removal and handling, dissection, fixation, dehydration, and embedding of the embryonic CNS. Most specimens were in an early stage of development (E11-E13), which, in case of the neopallial wall, is the preneural period. The tests produced methods that met the above criteria and identified the most common artifacts and their causes. Deformities of the cerebral hemispheres and separations between the brain and its coverings were usually caused by trauma during embryo removal and during handling before fixation. Changes in cellular volumes, especially swelling during fixation and dehydration, were the most important causes of histological artifacts. The procedures and methods that consistently produced the best light and electron microscopic preservation of the E11-E13 rat CNS are described. Fixation was best when the brains were treated with glutaraldehyde and s-collidine buffer, followed by osmium tetroxide in s-collidine buffer. A surprisingly beneficial effect of sodium chloride in the dehydrating alcohol was noted.
Aström
K E
KE
Laboratory of Experimental Neuropathology, NINDS, National Institutes of Health, Bethesda, Maryland 20892.
Webster
H D
HD
D000818:Animals; D001921:Brain / Q000196:embryology*; D003890:Desiccation; D004210:Dissection; D005333:Fetus / Q000033:anatomy & histology / Q000648:ultrastructure; D005404:Fixatives; D008853:Microscopy / Q000379:methods; D008854:Microscopy, Electron / Q000379:methods; D009997:Osmotic Pressure; D051381:Rats; D011919:Rats, Inbred Strains; D015203:Reproducibility of Results; D013048:Specimen Handling / Q000379:methods*
D005404:Fixatives