Molecular cloning and functional expression of a potassium channel cDNA isolated from a rat cardiac library.
A full-length K+ channel cDNA (RHK1) was isolated from a rat cardiac library using the polymerase chain reaction (PCR) method and degenerate oligonucleotide primers derived from K+ channel sequences conserved between Drosophila Shaker H4 and mouse brain MBK1. Although RHK1 was isolated from heart, its expression was found in both heart and brain. The RHK1-encoded protein, when expressed in Xenopus oocytes, gated a 4-aminopyridine (4-AP)-sensitive transient outward current. This current is similar to the transient outward current measured in rat ventricular myocytes with respect to voltage-dependence of activation and inactivation, time course of activation and inactivation, and pharmacology.
Tseng-Crank
J C
JC
Division of Biology, California Institute of Technology, Pasadena 91125.
Tseng
G N
GN
Schwartz
A
A
Tanouye
M A
MA
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015870:Gene Expression; D015723:Gene Library; D008564:Membrane Potentials; D008969:Molecular Sequence Data; D009206:Myocardium; D015221:Potassium Channels / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D014982:Xenopus laevis
D015221:Potassium Channels; D012333:RNA, Messenger; D004247:DNA