Regulation of glutathione peroxidase mRNA level by dietary selenium manipulation.
Glutathione peroxidase (GSH-Px) contains selenium at its active site as a selenocysteine moiety. We have shown that feeding mice a selenium-deficient diet for a long period caused a large decrease in the GSH-Px mRNA level as well as in GSH-Px activity both in the liver and kidneys (Toyoda, H., Himeno, S. and Imura, N. (1989) Biochim. Biophys. Acta 1008, 301-308). In the present study, the transcription rate of the GSH-Px gene was determined by a nuclear run-on assay using liver nuclei of mice fed a selenium-deficient or selenium-adequate diet. The results clearly demonstrate that the transcription rate of the GSH-Px gene was not changed by dietary selenium manipulation, indicating that dietary selenium regulates the level of GSH-Px mRNA in the post-transcriptional step.
Toyoda
H
H
Department of Public Health, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
Himeno
S
S
Imura
N
N
D000199:Actins / Q000235:genetics; D000818:Animals; D015152:Blotting, Northern; D004032:Diet; D015971:Gene Expression Regulation, Enzymologic; D005979:Glutathione Peroxidase / Q000235:genetics*; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000096:biosynthesis*; D012643:Selenium / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects
D000199:Actins; D012333:RNA, Messenger; D005979:Glutathione Peroxidase; D012643:Selenium