[Microcin R51 and a plasmid determining its synthesis].
A new microcin produced by an Citrobacter R51 strain has been detected. This antibiotic has been shown to inhibit the growth of a number of Gram-negative as well as Gram-positive strains of bacteria on the minimal medium plates. The properties of partially purified microcin were characterized. Constitutive synthesis of microcin is determined by a conjugative plasmid. The genes of microcin synthesis and immunity were cloned on a plasmid and plasmid vehicles. A physical map of the 12 kb fragment coding for the production of microcin R51 and immunity to this antibiotic is presented.
Kurepina
N E
NE
Khmel'
I A
IA
Lipasova
V A
VA
Zaĭtsev
D A
DA
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D001430:Bacteriocins / Q000096:biosynthesis* / Q000302:isolation & purification; D002954:Citrobacter / Q000235:genetics / Q000378:metabolism*; D005243:Feces / Q000382:microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests; D010957:Plasmids; D015183:Restriction Mapping; D013045:Species Specificity
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; C020411:microcin
Mikrotsin R51 i plazmida, opredeliaiushchaia ego sintez.