title: Labeling of proteins using [105Rh]Rh-4-(p-aminobenzyl)-diethylenetriamine.
abstract: A bifunctional ligand 4-(p-aminobenzyl)-diethylenetriamine has been synthesized. 105Rh complexes with this ligand were prepared with an overall yield of 79% at pH 9.0 in bicarbonate buffer. The preformed complex was converted to the isothiocyanate derivative using thiophosgene. Conjugation yields of 75% with IgG and 85% with HSA could be obtained for a 4 h conjugation reaction. Affinity chromatography of human-IgG coupled to the rhodium complex in an anti-human IgG agarose gel indicated no denaturation of the labeled protein. The procedure reported here can be adapted for the preparation of 105Rh-labeled antibodies for therapeutic applications.
authors.affiliation: Department of Chemistry, University of Missouri, Columbia 65211.
authors.forename: M R
authors.initials: MR
authors.lastname: Pillai
authors.forename: J M
authors.initials: JM
authors.lastname: Lo
authors.forename: C S
authors.initials: CS
authors.lastname: John
authors.forename: D E
authors.initials: DE
authors.lastname: Troutner
mesh_terms: D007074:Immunoglobulin G; D007553:Isotope Labeling / Q000379:methods; D009942:Organometallic Compounds; D011073:Polyamines / Q000138:chemical synthesis*; D011868:Radioisotopes; D012238:Rhodium; D012709:Serum Albumin
chemical_list: D007074:Immunoglobulin G; D009942:Organometallic Compounds; D011073:Polyamines; D011868:Radioisotopes; D012709:Serum Albumin; C065270:rhodium 4-(4-aminobenzyl)diethylenetriamine; D012238:Rhodium