A novel peroxisomal nonspecific lipid-transfer protein from Candida tropicalis. Gene structure, purification and possible role in beta-oxidation.
We have sequenced the nucleotides of the gene POX18 that encodes PXP-18, a major peroxisomal polypeptide inducible by oleic acid in the yeast Candida tropicalis. POX18 had a single open reading frame of 127 amino acids. Some 33% of the amino acid sequence of the predicted basic polypeptide (13,805 Da), was identical to that of the nonspecific lipid-transfer protein (sterol carrier protein 2) from rat liver. PXP-18, purified to near homogeneity from isolated peroxisomes, had an amino-terminal sequence identical to that of the predicted polypeptide except for the initiator methionine, and had nonspecific lipid-transfer activity comparable to that of its mammalian equivalents. Unexpectedly, PXP-18 lacked the cysteine residue thought to be essential for the activity of this protein in mammals. RNA blot analysis showed that the POX18 gene was expressed exclusively in cells grown on oleic acid, suggesting that PXP-18 has a role in the beta-oxidation of long-chain fatty acids. PXP-18 modulated acyl-coenzyme A oxidase activity at low pH.
Tan
H
H
Faculty of Integrated Arts and Sciences, Hiroshima University, Japan.
Okazaki
K
K
Kubota
I
I
Kamiryo
T
T
Utiyama
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002175:Candida / Q000032:analysis* / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D003545:Cysteine / Q000032:analysis / Q000502:physiology; D005800:Genes, Fungal; D008099:Liver / Q000032:analysis; D008830:Microbodies / Q000032:analysis*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010455:Peptides / Q000032:analysis / Q000235:genetics; D010940:Plant Proteins; D051381:Rats; D015183:Restriction Mapping
D002352:Carrier Proteins; D010455:Peptides; D010940:Plant Proteins; C072087:sterol carrier proteins; D003545:Cysteine