title: [The determination of trace amounts of protein in solutions containing surface-active substances].
abstract: A rapid and sensitive method for protein determination (0.5-16 micrograms) in samples of any volume containing various surfactants in concentration up to 1% is suggested. The method includes the protein acid denaturation, the solution of acid insoluble precipitate of detergent in ethanol (25-30%), the protein determination on nitrocellulose filter, dyeing by aminoblack 10 B, elution of dyed complex and colorimetric determination at 630 nm.
authors.forename: N P
authors.initials: NP
authors.lastname: Sirota
authors.forename: T V
authors.initials: TV
authors.lastname: Sirota
mesh_terms: D000580:Amido Black; D002138:Calibration; D004305:Dose-Response Relationship, Drug; D005374:Filtration / Q000295:instrumentation / Q000379:methods; D011489:Protein Denaturation / Q000187:drug effects; D011506:Proteins / Q000032:analysis*; D012996:Solutions; D013501:Surface-Active Agents / Q000032:analysis*
chemical_list: D011506:Proteins; D012996:Solutions; D013501:Surface-Active Agents; D000580:Amido Black
vernacular_title: Opredelenie mikrokolichestv belka v rastvorakh, soderzhashchikh poverkhnostno-aktivnye veshchestva.