Recognition of the product of a novel MHC TL region gene (27b) by a mouse gamma delta T cell receptor.
The gamma delta T cell receptor (TCR) derived from the mouse KN6 T cell hybridoma recognizes an autologous determinant encoded by a broadly expressed gene mapping in the TL region of the major histocompatibility complex (MHC). We have cloned the gene and demonstrated that it is a novel class I gene (designated 27b) belonging to a hitherto undescribed TL region gene cluster in strain C57BL/6. The BALB/c allele of 27b, gene T17c, is defective because it lacks an appropriate splice acceptor site, which explains the lack of recognition of BALB/c stimulator cells by the KN6 cells. We propose that gamma delta TCR and nonclassical MHC and MHC-related class I molecules have coevolved to recognize a conserved set of endogenous and foreign determinants.
Ito
K
K
Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge 02139.
Van Kaer
L
L
Bonneville
M
M
Hsu
S
S
Murphy
D B
DB
Tonegawa
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015342:DNA Probes; D005091:Exons; D005805:Genes, MHC Class I; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000276:immunology*; D007438:Introns; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D012326:RNA Splicing; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014162:Transfection
D015342:DNA Probes; D015395:Histocompatibility Antigens Class I; D011948:Receptors, Antigen, T-Cell