On the identity of the xenobiotic-metabolizing form(s) of cytochrome P-450 in endocrine organs.
The adrenal cortex, the testes and the ovary metabolize polycyclic aromatic hydrocarbons (PAHs), e.g., 7,12-dimethylbenz[a]anthracene (DMBA). These activities have previously been shown to involve the cytochrome P-450 monooxygenase system [18-20]. In attempt to identify the form(s) of cytochrome P-450 involved, microsomes from these endocrine organs were subjected to SDS-gel electrophoresis, followed by immunochemical analysis using the Western blot technique. Antisera raised against the purified rat hepatic cytochrome P-450 isozymes a, b + e, c, d and PB-PCNE were tested. It was concluded that the electrophoretic mobilities of the immunoreactive bands obtained were not identical to the mobilities of the purified isozymes cytochromes P-450 a, b, c, d, e or PB-PCNE. These results indicate that the PAH-metabolizing monooxygenase(s) in these endocrine organs may involve a novel form(s) of cytochrome P-450.
Bengtsson
M
M
Department of Biochemistry, Wallenberg Laboratory, University of Stockholm, Sweden.
Hallberg
E
E
Georgellis
A
A
Rydstrom
J
J
D000302:Adrenal Cortex / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D003577:Cytochrome P-450 Enzyme System / Q000302:isolation & purification / Q000378:metabolism*; D005260:Female; D007106:Immune Sera; D007074:Immunoglobulin G; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D008297:Male; D008861:Microsomes / Q000378:metabolism*; D010053:Ovary / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013737:Testis / Q000378:metabolism*; D015262:Xenobiotics / Q000378:metabolism*
D007106:Immune Sera; D007074:Immunoglobulin G; D007527:Isoenzymes; D015262:Xenobiotics; D003577:Cytochrome P-450 Enzyme System