Effects of biologically active tumour-promoting and non-promoting phorbol esters on in vitro growth of melanocytic cells.
Sapintoxin A (SAP A), a naturally occurring biologically active but non-promoting phorbol ester, acts as an effective in vitro mitogen for freshly derived human melanocytes. Seven days after addition of 50 nM SAP A there was a four to fivefold increase in melanocyte number over that observed in untreated control cultures comparable to that achieved with a 50 nM concentration of 12-0-tetradecanoylphorbol 13-acetate (TPA). The fluorescent stage 2 promoter sapintoxin D (SAP D) also supported the growth of these cells, with a 50 nM dose producing an increase in cell number comparable to that observed with 200 nM TPA. Similar results were obtained with an established, but non-tumorigenic, line of murine melanocytes. The same compounds exerted a potent anti-proliferative effect against transformed melanocyte lines of murine and human origin associated with morphological alterations and an increase in melanin production consistent with induced cytodifferentiation.
Brooks
G
G
Biology of Metastasis Laboratory, Imperial Cancer Research Fund, London, England.
Birch
M
M
Hart
I R
IR
D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000473:pathology; D002478:Cells, Cultured; D006801:Humans; D008297:Male; D008544:Melanocytes / Q000166:cytology* / Q000473:pathology; D010703:Phorbol Esters / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D010703:Phorbol Esters; C032016:sapintoxin A; C065374:sapintoxin D; D013755:Tetradecanoylphorbol Acetate