Regulation of the expression of the serine dehydratase gene in the kidney and liver of the rat.
Serine dehydratase was induced in the kidneys of normal rats by the administration of either glucagon or dexamethasone. The increase in enzyme activity was associated with an increase in both enzyme protein and its mRNA, which were determined respectively by Western blot and RNA blot analysis. No apparent differences were observed between kidney and liver in the molecular weights of serine dehydratase proteins and the sizes of their mRNAs. Although kidney serine dehydratase was dramatically induced by either glucagon or dexamethasone, the liver enzyme was induced by glucagon but not by dexamethasone alone in the intact rat. On the other hand, liver serine dehydratase was induced in starvation, diabetes mellitus, and a high-protein diet. The kidney enzyme could not be induced under any of these conditions.
Su
Y
Y
McArdle Laboratory for Cancer Research, Medical School, University of Wisconsin, Madison 53706.
Kanamoto
R
R
Miller
D A
DA
Ogawa
H
H
Pitot
H C
HC
D000818:Animals; D015153:Blotting, Western; D003907:Dexamethasone / Q000494:pharmacology; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology; D004790:Enzyme Induction; D015971:Gene Expression Regulation, Enzymologic; D005934:Glucagon / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects / Q000201:enzymology*; D012695:L-Serine Dehydratase / Q000096:biosynthesis / Q000235:genetics*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D012333:RNA, Messenger; D003907:Dexamethasone; D005934:Glucagon; D012695:L-Serine Dehydratase