The mouse ribosomal DNA promoter has more stringent requirements in vivo than in vitro.
Using mouse ribosomal DNA templates bearing polymerase I terminators to prevent transcriptional interference (S. L. Henderson, K. Ryan, and B. Sollner-Webb, Genes Dev. 3:212-223, 1989) and facilitate promoter analysis in intact cells, we demonstrate that a -140 promoter domain (as well as the core region) is essential for appreciable levels of initiation in vivo. This in vivo polymerase I promoter can also be detected in vitro but only under very stringent conditions.
Henderson
S L
SL
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Sollner-Webb
B
B
D000818:Animals; D002460:Cell Line; D002872:Chromosome Deletion; D004256:DNA Polymerase I / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D051379:Mice; D009154:Mutation; D011401:Promoter Regions, Genetic; D013698:Templates, Genetic; D013728:Terminator Regions, Genetic; D014162:Transfection
D004275:DNA, Ribosomal; D004256:DNA Polymerase I