A general method of optimizing automated DNA synthesis to decrease chemical consumption to less than half.
An automated DNA synthesis protocol (FC3) has been optimized to decrease the cost of chemical consumption by two- to threefold. Anion-exchange chromatography at pH 12.5 in a Mono Q (Pharmacia) column was used to analyze the oligonucleotides we synthesized. From these analyses, we formulated the FC3 program and a procedure by which a DNA synthesizer designer or operator can use 18-base-long homopolymers of A, G, C, and T to optimize the reaction times, reagent concentrations, solvent wash conditions, and the many steps in the synthesis cycle.
Yeung
A T
AT
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
Miller
C G
CG
D001331:Automation / Q000191:economics / Q000379:methods; D001483:Base Sequence; D002852:Chromatography, Ion Exchange; D004247:DNA / Q000138:chemical synthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000302:isolation & purification
D009841:Oligonucleotides; D004247:DNA