Direct high-performance liquid chromatographic determination of the enantiomers of alfuzosin in plasma on a second-generation alpha 1-acid glycoprotein chiral stationary phase.
A direct liquid chromatographic method was developed for the determination of the enantiomers of alfuzosin in human plasma, without derivatization, on a chiral alpha 1-acid glycoprotein column. The influence of pH, of uncharged organic solvents and of a cationic modifier (tetrabutylammonium) of the mobile phase on retention and enantioselectivity was evaluated. The enantiomers and an internal standard, structurally related to alfuzosin, were extracted from plasma with dichloromethane-diethyl ether from alkaline solution, then separated with a mobile phase of 0.025 M phosphate buffer (pH 7.4) containing 0.025 M tetrabutylammonium bromide-acetonitrile (94:6, v/v). The limit of quantification for each isomer was 1 ng/ml. The method has been applied to the determination of the pharmacokinetic profile of alfuzosin enantiomers in healthy volunteers after intravenous administration of the racemate.
Rouchouse
A
A
Synthélabo Recherche, L.E.R.S., Paris, France.
Manoha
M
M
Durand
A
A
Thenot
J P
JP
D002851:Chromatography, High Pressure Liquid; D006207:Half-Life; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009961:Orosomucoid; D011799:Quinazolines / Q000097:blood* / Q000493:pharmacokinetics; D013237:Stereoisomerism
D009961:Orosomucoid; D011799:Quinazolines; C047638:alfuzosin