title: Quantifying the effect of ischemia on epiphyseal growth in an extremity replant model.
abstract: Warm ischemia (21 degrees C) of 0, 2, 4, 6, or 8 hours was produced in a modified hindlimb preparation of 35 10-week-old Lewis rats by amputation. Subsequent microvascular anastomoses of each hindlimb to a syngeneic animal was done after which fluorochrome bone labels were administered 5 minutes after operation and on day 14 after operation. Epiphyseal plate growth (that between bone labels) was analyzed histomorphometrically and statistically. Epiphyseal plate growth was found to have a linear inverse relationship to ischemia time with a correlation coefficient (r) of -0.653 and high statistical significance (p less than 0.001). Overgrowth occurred at all ischemic periods except 8 hours, and vascular pedicle patency decreased as ischemia time progressed.
authors.affiliation: Department of Orthopaedic Surgery, Johns Hopkins University School of Medicine, Baltimore, Md.
authors.forename: L A
authors.initials: LA
authors.lastname: Carey
authors.forename: A P
authors.initials: AP
authors.lastname: Weiss
authors.forename: A J
authors.initials: AJ
authors.lastname: Weiland
mesh_terms: D000714:Anastomosis, Surgical / Q000379:methods; D000818:Animals; D006132:Growth Plate / Q000098:blood supply* / Q000254:growth & development; D006358:Hot Temperature; D007511:Ischemia / Q000503:physiopathology*; D051381:Rats; D011917:Rats, Inbred Lew; D012044:Regression Analysis; D013997:Time Factors; D014654:Vascular Patency