Deletion mapping of 39 random isolated Y-chromosome DNA fragments.
Thirty-nine recombinants isolated from a Y chromosome-specific library were deletion mapped. Seven deletion intervals were defined by hybridization of probes to DNA of eight individuals with aberrant Y chromosomes. Extreme cytogenetic limits of the deletion intervals were determined by in situ hybridization of one probe per deletion interval. Five intervals, with a total of twenty-five probes, were allocated to the long-arm euchromatic region. The probes described will be useful for characterization of aberrant Y chromosomes, in searching for expressed sequences on the Y chromosome, and for further study of the evolutionary relationship between the Y chromosome and other chromosomes.
Oosthuizen
C J
CJ
Department of Human Genetics, Tygerberg Hospital, Republic of South Africa.
Herbert
J S
JS
Vermaak
L K
LK
Brusnicky
J
J
Fricke
J
J
du Plessis
L
L
Retief
A E
AE
D002872:Chromosome Deletion; D002874:Chromosome Mapping; D015342:DNA Probes; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D012303:Ring Chromosomes; D014998:Y Chromosome / Q000648:ultrastructure*
D015342:DNA Probes