Optimization of magnetization transfer measurements: statistical analysis by stochastic simulation. Application to creatine kinase kinetics.
A systematic study was performed to optimize the accuracy of kinetic parameters derived from magnetization transfer measurements. Three techniques were investigated: time-dependent saturation transfer (TDST), saturation recovery (SRS), and inversion recovery (IRS). In the last two methods, one of the resonances undergoing exchange is saturated throughout the experiment. The three techniques were compared with respect to the accuracy of the kinetic parameters derived from experiments performed in a given, fixed, amount of time. Stochastic simulation of magnetization transfer experiments was performed to optimize experimental design. General formulas for the relative accuracies of the unidirectional rate constant (k) were derived for each of the three experimental methods. It was calculated that for k values between 0.1 and 1.0 s-1, T1 values between 1 and 10 s, and relaxation delays appropriate for the creatine kinase reaction, the SRS method yields more accurate values of k than does the IRS method. The TDST method is more accurate than the SRS method for reactions where T1 is long and k is large, within the range of k and T1 values examined. Experimental verification of the method was carried out on a solution in which the forward (PCr----ATP) rate constant (kf) of the creatine kinase reaction was measured.
Rydzy
M
M
Division of Biological Sciences, National Research Council of Canada, Ottawa, Ontario.
Deslauriers
R
R
Smith
I C
IC
Saunders
J K
JK
D000244:Adenosine Diphosphate / Q000493:pharmacokinetics; D003198:Computer Simulation; D003402:Creatine Kinase / Q000493:pharmacokinetics*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008433:Mathematics; D008954:Models, Biological; D010758:Phosphorus; D013269:Stochastic Processes; D013997:Time Factors
D010758:Phosphorus; D000244:Adenosine Diphosphate; D003402:Creatine Kinase