Blastogenesis and polyclonal immunoglobulin synthesis in murine spleen cells stimulated with lipopolysaccharide, lipid A and acid-degraded polysaccharide from Fusobacterium nucleatum.
Lipopolysaccharide of Fusobacterium nucleatum strain Fevl was split by acid hydrolysis. The split products, i.e. lipid A and degraded polysaccharide were mitogenic for murine spleen cells as measured by uptake of [3H]thymidine. The uptake of [3H]thymidine was dose-dependent. Incubation of spleen cells with stimulants for 3 days resulted in a polyclonal activation of immunoglobulin synthesis. Higher mitogenic response and immunoglobulin production were found in spleen cells of athymic mice compared to those of thymic mice. The activity of lipid A in stimulating immunoglobulin synthesis was comparable with the parent lipopolysaccharide-Fevl, the degraded polysaccharide being the less potent stimulator.
Sveen
K
K
Department of Microbiology and Immunology, Gade Institute, University of Bergen, Norway.
Hofstad
T
T
D000818:Animals; D004247:DNA / Q000096:biosynthesis; D005673:Fusobacterium / Q000276:immunology*; D007136:Immunoglobulins / Q000096:biosynthesis*; D007700:Kinetics; D008050:Lipid A / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011135:Polysaccharides, Bacterial / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology
D007136:Immunoglobulins; D008050:Lipid A; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D004247:DNA