Optimized radiolabelling of pig granulocytes with indium-111 tropolonate.
Standard labelling methods using 111In-tropolonate in plasma resulted in lower labelling efficiency (LE) with pig granulocytes than with human cells. Addition of acid-citrate-dextrose (ACD) to cell-free plasma in a ratio of 1:10 tripled the LE to a value which was not significantly different from that obtained in saline. Under optimized conditions, LE was 67 +/- 2% (mean +/- SE) with pig granulocytes, which remained lower than that obtained with human cells but was adequate for nuclear imaging studies of granulocyte deposition in the pig.
Triandafillou
J M
JM
Division of Vascular Surgery, Ottawa Civic Hospital, Ontario, Canada.
Fair
B
B
Petrovic
V
V
Ballinger
J R
JR
Cole
C W
CW
D000818:Animals; D003508:Cycloheptanes; D006098:Granulocytes; D007205:Indium Radioisotopes; D007553:Isotope Labeling / Q000379:methods*; D009942:Organometallic Compounds; D013552:Swine; D014334:Tropolone / Q000031:analogs & derivatives
D003508:Cycloheptanes; D007205:Indium Radioisotopes; D009942:Organometallic Compounds; C043895:indium tris(tropolonate); D014334:Tropolone