Diadenosine tetraphosphate (Ap4A) levels in HL-60 cells during differentiation into granulocytes and monocytes.
1. Diadenosine tetraphosphate (Ap4A) levels were determined in HL-60 cells differentiating into granulocytes or monocytes after treatment for 0-7 days with retinoic acid (RA) or 4-beta-phorbol-12-myristate-13-acetate (PMA) respectively. 2. The levels increased significantly compared to untreated control cells within 2 days and then declined again. 3. In RA treated cells the levels finally decreased far below those of untreated HL-60 cells and became equal to those found in human granulocytes. 4. PMA treatment had no effect on Ap4A levels in human granulocytes. 5. A possible interaction between Ap4A and ADP-ribosyl transferase is discussed.
Andersson
M
M
Department of Zoophysiology, University of Lund, Sweden.
Oredsson
S M
SM
Olsson
H
H
Bergstrand
H
H
D002454:Cell Differentiation; D015226:Dinucleoside Phosphates / Q000378:metabolism*; D006098:Granulocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism* / Q000473:pathology; D009000:Monocytes / Q000166:cytology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
D015226:Dinucleoside Phosphates; C020733:diadenosine tetraphosphate; D014212:Tretinoin; D013755:Tetradecanoylphorbol Acetate