Changes of lens protein particles during development and reversal of galactose cataracts. Study by laser scattering spectroscopy.
3-week-old Wistar rats weighing about 50 g were fed a 38% galactose diet which was subsequently replaced with a normal diet. We measured the protein particle diameters and the scattered light intensity in the transparent part of galactose cataractous lenses. The scattered light intensity of small particles with a diameter of approximately 0.02 microns, corresponding to alpha-crystallin, decreased with a galactose diet, whereas it increased with a normal diet.
Kaneda
M
M
Department of Ophthalmology, School of Medicine, Fujita-Gakuen Health University, Aichi, Japan.
Majima
Y
Y
Hattori
H
H
Torii
H
H
D000818:Animals; D002386:Cataract / Q000378:metabolism*; D003459:Crystallins / Q000378:metabolism*; D005690:Galactose; D007908:Lens, Crystalline / Q000378:metabolism*; D008027:Light; D008297:Male; D010316:Particle Size; D051381:Rats; D011919:Rats, Inbred Strains; D012542:Scattering, Radiation
D003459:Crystallins; D005690:Galactose