HLA-DP beta and susceptibility to multiple sclerosis: an analysis of caucasoid and Japanese patient populations.
Nonradioactive sequence-specific oligonucleotide (SSO) probes specific for the HLA-DP beta locus have been used in a simple dot-blot assay to DP beta-type samples amplified by the polymerase chain reaction (pcr) from Caucasoid (n = 24) and Japanese (n = 23) patients with multiple sclerosis (ms) as well as ethnically matched controls. In contrast to previous reports, no DP beta allele was found to be increased in either patient population. However, the results do show a dramatic difference in the allele frequencies between the two control populations, further emphasizing the need for ethnically matched controls in studies of HLA and disease.
Begovich
A B
AB
Department of Human Genetics, Cetus Corporation, Emeryville, California 94608.
Helmuth
R C
RC
Oksenberg
J R
JR
Sakai
K
K
Tabira
T
T
Sasazuki
T
T
Steinman
L
L
Erlich
H A
HA
D000483:Alleles; D000595:Amino Acid Sequence; D044466:Asian People / Q000235:genetics; D001483:Base Sequence; D015343:DNA Probes, HLA; D005787:Gene Frequency; D008040:Genetic Linkage; D006682:HLA-DP Antigens / Q000235:genetics*; D006801:Humans; D007564:Japan; D008969:Molecular Sequence Data; D009103:Multiple Sclerosis / Q000235:genetics / Q000276:immunology*; D044465:White People / Q000235:genetics
D015343:DNA Probes, HLA; D006682:HLA-DP Antigens