title: Effects of recombinant eel growth hormone on the uptake of [35S]sulfate by ceratobranchial cartilages of the Japanese eel, Anguilla japonica.
abstract: Effects of growth hormone (GH) on the synthesis of mucopolysaccharide by ceratobranchial cartilages of the Japanese eel, Anguilla japonica, were examined by monitoring the in vitro uptake of [35S]sulfate. The [35S]sulfate uptake decreased rapidly to one-third of the initial level during the first 3 days after hypophysectomy, and decreased gradually thereafter. When hypophysectomized eels were injected intramuscularly with recombinant eel GH (2 micrograms/g), the plasma GH concentrations increased maximally after 6 hr, and declined rapidly thereafter. On the other hand, the sulfate uptake increased significantly after 12 hr, and high levels were maintained until 48 hr. The stimulating effect of GH was dose dependent (0.02-2 micrograms/g). However, the addition of eel GH (0.05-5 micrograms/ml) to the culture medium did not affect the sulfate uptake by hypophysectomized eel cartilages, suggesting that the stimulative action of GH on the sulfate uptake by the cartilages is indirect.
authors.affiliation: Ocean Research Institute, University of Tokyo, Japan.
authors.forename: C M
authors.initials: CM
authors.lastname: Duan
authors.forename: Y
authors.initials: Y
authors.lastname: Inui
mesh_terms: D000811:Anguilla / Q000378:metabolism*; D000818:Animals; D002356:Cartilage / Q000187:drug effects / Q000378:metabolism*; D013006:Growth Hormone / Q000097:blood / Q000494:pharmacology*; D007016:Hypophysectomy; D007700:Kinetics; D011994:Recombinant Proteins / Q000494:pharmacology; D013431:Sulfates / Q000378:metabolism*; D013462:Sulfur Radioisotopes
chemical_list: D011994:Recombinant Proteins; D013431:Sulfates; D013462:Sulfur Radioisotopes; D013006:Growth Hormone