Isolation and characterization of DNA sequences from flow-sorted human chromosome 22 libraries.
Two flow-sorted chromosome 22 libraries were used to isolate DNA sequences specific for chromosome 22. 45-phage DNAs were probed against human genomic DNA. 12 of them showed unique or low-copy character. Using digested DNA from rodent-human hybrid cell lines, 3 of the 12 recombinants were assigned unique to chromosome 22 and regionally mapped. 1 clone mapped to 22pter-q11, 1 clone to 22q12-qter and 1 clone, for which in situ hybridization was performed, to 22q13.1. 2 low-copy probes, 1 of them displaying polymorphisms in MspI and TaqI digests of individual DNAs, must have similar sequences on 22 and additional chromosomes. Furthermore, a highly repetitive DNA representing a compound locus of some hundred kilobases on chromosome 22 was isolated. These 6 probes may provide useful tools for studying the structure and function of this small chromosome involved in a relatively high number of inherited and acquired diseases.
Metzdorf
R
R
Department of Human Genetics, Saar University, Homburg, FRG.
Herzog
R
R
Göttert
E
E
Blin
N
N
D002892:Chromosomes, Human, Pair 22; D015342:DNA Probes / Q000302:isolation & purification*; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D005434:Flow Cytometry; D015723:Gene Library; D006801:Humans; D010375:Pedigree; D011110:Polymorphism, Genetic
D015342:DNA Probes; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII