Isolation and structure of the pectin lyase D-encoding gene from Aspergillus niger.
The filamentous fungus, Aspergillus niger, produces a number of extracellular pectin-degrading enzymes. We present here the isolation and the complete nucleotide sequence of the gene, pelD, coding for a pectin lyase D (PLD), which was previously described as pectin lyase I (Van Houdenhoven, Ph.D. Thesis, Wageningen, 1975). The deduced amino acid (aa) sequence corresponds to 373 aa residues including a signal peptide of 19 aa. The coding region is interrupted by four short introns (57-65 bp). The nucleotide sequence of the 5'- and 3'-flanking regions is also presented and shows no unusual features. By comparing the deduced aa sequences of the A. niger PLD and a number of bacterial pectate lyases, short regions of homology were found despite the different substrate specificities (high methoxyl-pectin versus low methoxyl-pectin or polygalacturonate) of these enzymes.
Gysler
C
C
Biotechnology Department Ciba Geigy Ltd., CH-4002 Basle, Switzerland.
Harmsen
J A
JA
Kester
H C
HC
Visser
J
J
Heim
J
J
D000595:Amino Acid Sequence; D001234:Aspergillus niger / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D003062:Codon / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D005800:Genes, Fungal; D007438:Introns; D008969:Molecular Sequence Data; D010957:Plasmids; D011133:Polysaccharide-Lyases / Q000235:genetics*; D021382:Protein Sorting Signals / Q000235:genetics; D012326:RNA Splicing; D012331:RNA, Fungal / Q000235:genetics; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D003062:Codon; D004271:DNA, Fungal; D021382:Protein Sorting Signals; D012331:RNA, Fungal; D011133:Polysaccharide-Lyases; C027349:pectin lyase; C023911:pectate lyase