Inhibition of mitochondrial palmitate oxidation by calmodulin antagonists.
1. The effect of calmodulin antagonists on the rate of palmitate oxidation by isolated rat liver mitochondria was studied. 2. In the presence of 100 microM amitriptyline, chlorpromazine, prenylamine, N-(4-aminobutyl)-5-chloro-2-naphthalenesulfonamide or N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide, palmitate oxidation was inhibited by 17, 34, 49, 31 and 37%, respectively. 3. The degree of inhibition of palmitate oxidation exerted by these chemical compounds did not appear to correlate appreciably with changes in mitochondrial membrane fluidity.
Loo
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Lipid Nutrition Laboratory, Beltsville Human Nutrition Research Center, MD 20705.
Berlin
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Smith
J T
JT
D000818:Animals; D002147:Calmodulin / Q000037:antagonists & inhibitors*; D066298:In Vitro Techniques; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects*; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction; D010168:Palmitates / Q000378:metabolism*; D010169:Palmitic Acids / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D002147:Calmodulin; D010168:Palmitates; D010169:Palmitic Acids