Activity of 1,2-dimethylhydrazine in the mouse bone marrow micronucleus assay using a triple- and a single-dosing protocol.
In the present study the ability of 1,2-dimethylhydrazine (DMH) to induce micronuclei in mouse bone marrow erythrocytes was investigated using 2 different dosing regimens. DMH caused an increase in micronuclei in both male and female mice following single administration and sampling after 24 h. The effect was more pronounced in female than in male animals. Triple administration of DMH at concentrations corresponding to 80, 40 and 20% of the median lethal dose (MLD) did not increase the incidence of micronuclei in either sex. Small increases in micronucleus incidence were observed after triple dosing at 100% of the MLD value. These results suggest that a future micronucleus protocol should include animals of both sexes and single and repeated administration of the test substance.
Meli
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C
LSR Roma Toxicology Centre, Italy.
Seeberg
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AH
D019813:1,2-Dimethylhydrazine; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects*; D001854:Bone Marrow Cells; D003520:Cyclophosphamide / Q000494:pharmacology; D004127:Dimethylhydrazines / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D004912:Erythrocytes / Q000187:drug effects* / Q000648:ultrastructure; D005260:Female; D008297:Male; D008763:Methylhydrazines / Q000494:pharmacology*; D051379:Mice; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects*; D015162:Micronucleus Tests / Q000379:methods; D009153:Mutagens / Q000494:pharmacology*; D012737:Sex Factors
D004127:Dimethylhydrazines; D008763:Methylhydrazines; D009153:Mutagens; D003520:Cyclophosphamide; D019813:1,2-Dimethylhydrazine