Enhancing effect of centrifugation on isolation of influenza virus from clinical specimens.
The use of centrifugation (700 x g, 60 min) in a plaque assay markedly increased (mean, 2.9-fold) the infectivity of all 42 influenza virus strains tested, compared with no centrifugation. Of 13 influenza virus strains isolated from 390 clinical specimens, 9 (69%) were efficiently isolated by the centrifugation assay compared with conventional culture methods. The centrifugation assay may be useful for isolating the influenza virus from clinical specimens.
Seno
M
M
Division of Microbiology, Hiroshima Prefectural Institute of Public Health, Japan.
Kanamoto
Y
Y
Takao
S
S
Takei
N
N
Fukuda
S
S
Umisa
H
H
D002498:Centrifugation; D005069:Evaluation Studies as Topic; D006801:Humans; D009975:Orthomyxoviridae / Q000302:isolation & purification*; D010948:Viral Plaque Assay; D014773:Virology / Q000379:methods*