Whole complement hemolytic activity (CH50) following infusion of stroma-free and polyhemoglobin solutions in rats.
We developed a method to determine whole complement hemolytic activity (CH50) in rat plasma using a modification of the method of Mayer(1). Plasma CH50 level in 30 male Sprague Dawley rats was 1000 +/- 200 units. Following infusions with human and rat stroma-free hemoglobin or polyhemoglobin solutions, CH50 did not change significantly in rats when compared to albumin infused rats. On the other hand, CH50 significantly decreased in the group receiving membrane stroma and bacterial endotoxins.
Ning
J
J
Artificial Cells and Organs Research Centre, McGill University, Montreal, Canada.
Chang
T M
TM
D000818:Animals; D001802:Blood Substitutes / Q000633:toxicity; D003167:Complement Activation / Q000187:drug effects; D015941:Complement Hemolytic Activity Assay / Q000379:methods*; D004731:Endotoxins / Q000633:toxicity; D006454:Hemoglobins; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012996:Solutions
D001802:Blood Substitutes; D004731:Endotoxins; D006454:Hemoglobins; D012996:Solutions; C037158:polyhemoglobin