Isolation and microsequence analysis of a novel form of neuromedin U from guinea pig small intestine.
A multidimensional chromatographic regimen has been used to isolate and purify a peptide showing immunoreactivity for neuromedin U from guinea pig small intestine. Microsequence Edman N-terminal analysis and C-terminal analysis by enzymatic digestion showed this peptide to be a nonapeptide with the following sequence: H-Gly-Tyr-Phe-Leu-Phe-Arg-Pro-Arg-Asn-NH2. The C-terminal octapeptide of this sequence is the same as porcine NMU-8, and the C-terminal heptapeptide is identical to rat NMU(17-23).
Murphy
R
R
Department of Anatomy and Histology, School of Medicine, Flinders University, Bedford Park, S.A., Australia.
Turner
C A
CA
Furness
J B
JB
Parker
L
L
Giraud
A
A
D000595:Amino Acid Sequence; D000818:Animals; D002850:Chromatography, Gel; D006168:Guinea Pigs; D007421:Intestine, Small / Q000032:analysis*; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000302:isolation & purification; D011863:Radioimmunoassay; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013552:Swine
D009479:Neuropeptides; C056900:neuromedin U