Spectrophotometric correction for bile pigments in the thiobarbituric acid test for malondialdehyde-like substances in plasma.
A simple method is proposed to improve specificity when estimating malondialdehyde-like components of plasma thiobarbituric acid-reactivity, especially with regard to the considerable contribution of bilirubin. The spectrophotometric method comprises measurement of A485, A532 and A560 instead of only A532. A correction formula is then applied to calculate the portion of the A532 which originates from malondialdehyde-like substances. Using this method, normal values for malondialdehyde average 0.84 mumol/l.
Hendriks
T
T
Department of General Surgery, St Radboud University Hospital, Nijmegen, The Netherlands.
Assmann
R F
RF
D001654:Bile Pigments; D006801:Humans; D008314:Malonates / Q000097:blood*; D008315:Malondialdehyde / Q000097:blood*; D010949:Plasma; D013054:Spectrophotometry, Atomic / Q000379:methods; D013858:Thiobarbiturates
D001654:Bile Pigments; D008314:Malonates; D013858:Thiobarbiturates; D008315:Malondialdehyde; C029684:thiobarbituric acid