title: An analysis of megakaryocytopoiesis in the C3H mouse: an animal model whose megakaryocytes have 32N as the modal DNA class.
abstract: The modal DNA content of normal marrow megakaryocytes from species so far examined usually has been reported to be 16N. In this report we describe an exception in the C3H mouse whose megakaryocytes have a modal DNA content of 32N. Female C3H/HEN mice had an average DNA content distribution of 14% 8N, 37% 16N, 43% 32N, and 6% 64N. Male C3H/HEN mice had somewhat higher proportions of 32N and 64N megakaryocytes (average DNA content distribution of 12% 8N, 29% 16N, 47% 32N, and 12% 64N) than females. All 11 other mouse strains examined had 16N as the modal megakaryocyte DNA content, although the proportions in the various polyploid DNA classes showed some strain variation. Megakaryocyte size was similar among all 12 strains evaluated, and mean platelet volume (MPV) of C3H/HEN mice differed from only 1 of the other 4 strains analyzed. Platelet counts of C3H/HEN mice were similar to those of six, and slightly but significantly lower than those of five other mouse strains examined. Compared with megakaryocyte concentrations of other mouse strains studied, that of C3H/HEN mice was similar to seven, somewhat higher than one, and slightly lower than three strains. Offspring from reciprocal matings of C57BL/6 and C3H/HEN mice had megakaryocyte DNA distributions intermediate between those of the parent strains, suggesting that a higher gene dosage of some component is responsible for the right-shifted megakaryocyte DNA content distribution phenotype of C3H mice. The proportions of 32N and 64N megakaryocytes increased in C3H/HEN mice in response to acute thrombocytopenia, as did those of CBA/CAJ mice used as a comparative strain. In summary, megakaryocytes of the C3H mouse have a higher average DNA content but similar platelet count, MPV, and megakaryocyte size and concentration as those of most other mouse strains. These results suggest that the number of platelets produced per unit of C3H megakaryocyte DNA is less than that for other mice.
authors.affiliation: Department of Hematology-Oncology, St. Jude Children's Research Hospital, Memphis 38101.
authors.forename: C W
authors.initials: CW
authors.lastname: Jackson
authors.forename: S A
authors.initials: SA
authors.lastname: Steward
authors.forename: P J
authors.initials: PJ
authors.lastname: Chenaille
authors.forename: R A
authors.initials: RA
authors.lastname: Ashmun
authors.forename: T P
authors.initials: TP
authors.lastname: McDonald
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D004247:DNA / Q000032:analysis; D004195:Disease Models, Animal; D005260:Female; D030342:Genetic Diseases, Inborn / Q000097:blood / Q000235:genetics; D008297:Male; D008533:Megakaryocytes / Q000032:analysis / Q000166:cytology*; D051379:Mice; D008809:Mice, Inbred C3H / Q000235:genetics*; D011003:Ploidies
chemical_list: D004247:DNA