[A computerized method for optimization of radioimmunoassays].
The authors have developed a one step quantitative method for radioimmunoassay optimization. The method is rapid and necessitates only to perform a series of saturation curves with different titres of the antiserum. After calculating the saturation point at several antiserum titres using the Scatchard plot, the authors have produced a table that predicts the main characteristics of the standard curve (Bo/T, Bo and T) that will prevail for any combination of antiserum titre and percentage of sites saturation. The authors have developed a microcomputer program able to interpolate all the data needed to produce such a table from the results of the saturation curves. This computer program permits also to predict the sensitivity of the assay at any experimental conditions if the antibody does not discriminate between the labeled and the non labeled antigen. The authors have tested the accuracy of this optimization table with two in house RIA systems: 17-beta-estradiol, and hLH. The results obtained experimentally, including sensitivity determinations, were concordant with those predicted from the optimization table. This method accelerates and improves greatly the process of optimization of radioimmunoassays.
Rousseau
F
F
Service de Biochimie et Centre de Recherche de l'Hôpital Saint-François-d'Assise, Québec, Canada.
Forest
J C
JC
D006801:Humans; D007106:Immune Sera; D008838:Microcomputers; D011863:Radioimmunoassay; D012984:Software; D012986:Software Validation
D007106:Immune Sera
Informatisation de la mise au point des radio-immunodosages.