title: NADPH:O2 oxidoreductase of human eosinophils in the cell-free system.
abstract: The NADPH oxidase of human eosinophils, measured in the cell-free system, shows the same characteristics as the enzyme from human neutrophils. All proteins required for activity of the enzyme are expressed in eosinophils at a higher level than in neutrophils. Eosinophils isolated from patients with chronic granulomatous disease show the same molecular defects as the neutrophils from these patients.
authors.affiliation: Central Laboratory, Netherlands Red Cross Blood Transfusion Service, Amsterdam.
authors.forename: B G
authors.initials: BG
authors.lastname: Bolscher
authors.forename: L
authors.initials: L
authors.lastname: Koenderman
authors.forename: A T
authors.initials: AT
authors.lastname: Tool
authors.forename: P M
authors.initials: PM
authors.lastname: Stokman
authors.forename: D
authors.initials: D
authors.lastname: Roos
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D015153:Blotting, Western; D002462:Cell Membrane / Q000201:enzymology; D002474:Cell-Free System; D004789:Enzyme Activation / Q000187:drug effects; D004804:Eosinophils / Q000201:enzymology*; D006105:Granulomatous Disease, Chronic / Q000201:enzymology; D006801:Humans; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D019255:NADPH Oxidases; D009504:Neutrophils / Q000201:enzymology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
chemical_list: D012967:Sodium Dodecyl Sulfate; D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases; D000469:Alkaline Phosphatase