NADPH:O2 oxidoreductase of human eosinophils in the cell-free system.
The NADPH oxidase of human eosinophils, measured in the cell-free system, shows the same characteristics as the enzyme from human neutrophils. All proteins required for activity of the enzyme are expressed in eosinophils at a higher level than in neutrophils. Eosinophils isolated from patients with chronic granulomatous disease show the same molecular defects as the neutrophils from these patients.
Bolscher
B G
BG
Central Laboratory, Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Koenderman
L
L
Tool
A T
AT
Stokman
P M
PM
Roos
D
D
D000469:Alkaline Phosphatase / Q000378:metabolism; D015153:Blotting, Western; D002462:Cell Membrane / Q000201:enzymology; D002474:Cell-Free System; D004789:Enzyme Activation / Q000187:drug effects; D004804:Eosinophils / Q000201:enzymology*; D006105:Granulomatous Disease, Chronic / Q000201:enzymology; D006801:Humans; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D019255:NADPH Oxidases; D009504:Neutrophils / Q000201:enzymology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
D012967:Sodium Dodecyl Sulfate; D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases; D000469:Alkaline Phosphatase