title: Taxonomic studies on methylotrophic bacteria by 5S ribosomal RNA sequencing.
abstract: Nucleotide sequences of 5S ribosomal RNA (rRNA) isolated from 19 strains of Gram-negative methylotrophic bacteria were determined. Comparison of these sequences allowed construction of a tentative phylogenetic tree and showed that the bacteria analysed belong to the Proteobacteria and fell into several clusters, including obligate methanotrophs, obligate methylotrophs and several groups of facultative methylotrophs. Taxonomic relations between methylotrophic and non-methylotrophic bacteria are discussed, and the polyphyletic nature of methylotrophy as a taxonomic feature is highlighted.
authors.affiliation: Institute of Microbiology, Academy of Sciences, Moscow, USSR.
authors.forename: E S
authors.initials: ES
authors.lastname: Bulygina
authors.forename: V F
authors.initials: VF
authors.lastname: Galchenko
authors.forename: N I
authors.initials: NI
authors.lastname: Govorukhina
authors.forename: A I
authors.initials: AI
authors.lastname: Netrusov
authors.forename: D I
authors.initials: DI
authors.lastname: Nikitin
authors.forename: Y A
authors.initials: YA
authors.lastname: Trotsenko
authors.forename: K M
authors.initials: KM
authors.lastname: Chumakov
mesh_terms: D001483:Base Sequence; D008772:Methylococcaceae / Q000145:classification* / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000032:analysis*; D012341:RNA, Ribosomal, 5S / Q000032:analysis*; D009626:Terminology as Topic
chemical_list: D012335:RNA, Ribosomal; D012341:RNA, Ribosomal, 5S