title: Optimized radiolabelling of pig granulocytes with indium-111 tropolonate.
abstract: Standard labelling methods using 111In-tropolonate in plasma resulted in lower labelling efficiency (LE) with pig granulocytes than with human cells. Addition of acid-citrate-dextrose (ACD) to cell-free plasma in a ratio of 1:10 tripled the LE to a value which was not significantly different from that obtained in saline. Under optimized conditions, LE was 67 +/- 2% (mean +/- SE) with pig granulocytes, which remained lower than that obtained with human cells but was adequate for nuclear imaging studies of granulocyte deposition in the pig.
authors.affiliation: Division of Vascular Surgery, Ottawa Civic Hospital, Ontario, Canada.
authors.forename: J M
authors.initials: JM
authors.lastname: Triandafillou
authors.forename: B
authors.initials: B
authors.lastname: Fair
authors.forename: V
authors.initials: V
authors.lastname: Petrovic
authors.forename: J R
authors.initials: JR
authors.lastname: Ballinger
authors.forename: C W
authors.initials: CW
authors.lastname: Cole
mesh_terms: D000818:Animals; D003508:Cycloheptanes; D006098:Granulocytes; D007205:Indium Radioisotopes; D007553:Isotope Labeling / Q000379:methods*; D009942:Organometallic Compounds; D013552:Swine; D014334:Tropolone / Q000031:analogs & derivatives
chemical_list: D003508:Cycloheptanes; D007205:Indium Radioisotopes; D009942:Organometallic Compounds; C043895:indium tris(tropolonate); D014334:Tropolone