Induction of Mn2+/H+ antiport in chicken erythrocytes by intracellular Mg2+ and Mn2+.
Chicken erythrocytes preloaded with Mg2+ exchange one extracellular Mn2+ for two intracellular H+. Chicken erythrocytes preloaded with Mn2+ alone or with Mg2+ plus Mn2+ performed efflux of Mn2+, which was higher at pH 6 than at pH 7.4, indicating reversibility of Mn2+/H+ antiport. Mn2+/H+ antiport was not inhibited by 1 mM KCN plus 1 mM iodoacetic acid or 1 mM amiloride. Mn2+ influx was activated by anions. Mn2+ efflux via Mn2+/H+ antiport was inhibited by competition between H+ and K+.
Günther
T
T
Institute of Molecular Biology and Biochemistry, Free University of Berlin, FRG.
Vormann
J
J
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002645:Chickens; D004912:Erythrocytes / Q000378:metabolism*; D006859:Hydrogen / Q000097:blood; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D008274:Magnesium / Q000097:blood* / Q000494:pharmacology; D008345:Manganese / Q000097:blood* / Q000494:pharmacology
D008345:Manganese; D006859:Hydrogen; D008274:Magnesium