Cultivation of Clinostomum marginatum (Digenea: Clinostomatidae) metacercariae in vitro, in chick embryo and in mouse coelom.
Metacercariae of Clinostomum marginatum obtained from naturally infected Perca flavescens were cultured by 5 methods. In vitro cultivation at 37 C in twice daily changes of Tyrode's, Na-poor Tyrode's, and Eagle's media failed to produce ovigerous adults after 4-5 days. Metacercariae placed on the chorioallantois of week-old chick embryos at 37 C migrated to the albumen. Only 8 of 123 worms recovered were ovigerous after 4-7 days in ovo. Best success occurred with metacercariae injected in groups of 4 or 6 into the abdominal cavities of male A/J mice. Despite liver lesions and strong inflammatory responses in the host, all 174 worms recovered were ovigerous after 4-8 days. None of 41 mice died prematurely from the procedure or the parasites.
Larson
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OR
Department of Biology, University of North Dakota, Grand Forks 58202.
Uglem
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GL
D000818:Animals; D002642:Chick Embryo; D003470:Culture Media; D005393:Fish Diseases / Q000469:parasitology*; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D010471:Perches / Q000469:parasitology*; D010473:Perciformes / Q000469:parasitology*; D014200:Trematoda / Q000254:growth & development*; D014201:Trematode Infections / Q000469:parasitology / Q000662:veterinary*
D003470:Culture Media