title: [Penetrating keratoplasty with long-term cryopreserved corneas].
abstract: Since March 1986, the authors studied the simplified corneal cryopreservation technique with their "QL Bio-Freezer". After successful animal experiments, 34 patients (35 eyes) were performed penetrating keratoplasty with human cadaver corneas cryopreserved 9 to 473 days, averaging 108 days. After following-ups of 1 1/2 to 28 months, 22 of the corneal grafts remained clear, 6 semitransparent, and 7 cloudy. A total of 25 eyes had their visual acuity improved significantly. The results show that the cornea cryopreservation technique with the simplified "two-step" method is a noteworthy clinical practice.
authors.affiliation: Department of Ophthalmology, Affiliated Hospital to Qingdao Medical College, Shandong Province.
authors.forename: C F
authors.initials: CF
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D003315:Cornea; D015925:Cryopreservation; D004728:Endothelium, Corneal / Q000648:ultrastructure; D005260:Female; D006801:Humans; D015948:Keratoplasty, Penetrating; D008297:Male; D008875:Middle Aged; D009926:Organ Preservation; D011184:Postoperative Period; D011817:Rabbits