[A study on identification of atypical and R phase strains of Brucella].
This paper reports a comprehensive study on taxonomy of Brucella. In which 17 atypical and R phase strains were identified by using the routine methods, 6 groups of phages and oxidative metabolic tests. 4 McAb of Brucella were developed and applied for identification. Meanwhile OMP profiles of S phase of B. abortus, B. melitensis and B. suis were compared during identification of them. The results show that a comprehensive identification with routine methods, phage-typing and oxidative metabolic tests are able to determine these strains if they are species or biotypes. Several strains of Brucella McAb and OMP profiles of Brucella were firstly applied for identification of atypical and R strains, and provided some possibility in the application of identification of Brucella by using these two methods.
Shang
D Q
DQ
Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D001434:Bacteriophage Typing; D002002:Brucella / Q000032:analysis / Q000145:classification* / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans
D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins