Rates of cholesterol, ubiquinone, dolichol and dolichyl-P biosynthesis in rat brain slices.
Slices from the brain and liver of rats were prepared and upon incubation exhibited a continuous and high capacity for incorporation of radioactive precursors into proteins and lipids. Using [3H]mevalonate as precursor, the rates of biosynthesis of cholesterol, ubiquinone, dolichol and dolichyl-P in brain slices were determined and found to be 5.5, 0.25, 0.0093 and 0.0091 nmol/h/g, respectively. Dolichol and dolichyl-P accumulate to a limited extent, but almost all of these lipids in the brain originate from de novo synthesis. The calculated half-lives for cholesterol, ubiquinone, dolichol and dolichyl-P were 4076, 90, 1006 and 171 h, respectively. The results indicate that lipids formed via the mevalonate pathway in the brain have an active and independently regulated biosynthesis.
Andersson
M
M
Department of Cellular and Neuropathology, Huddinge Hospital, Karolinska Institutet, Sweden.
Elmberger
P G
PG
Edlund
C
C
Kristensson
K
K
Dallner
G
G
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002784:Cholesterol / Q000096:biosynthesis*; D004288:Dolichol Phosphates / Q000378:metabolism*; D004286:Dolichols / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D010743:Phospholipids / Q000096:biosynthesis; D011106:Polyisoprenyl Phosphates / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014451:Ubiquinone / Q000378:metabolism*
D004288:Dolichol Phosphates; D004286:Dolichols; D009419:Nerve Tissue Proteins; D010743:Phospholipids; D011106:Polyisoprenyl Phosphates; D014451:Ubiquinone; D002784:Cholesterol