Freezing rabbit semen by the use of BF5 diluent.
Three experiments were carried out to find the optimal concentration of DMSO and glycerol in BF5 diluent for freezing rabbit spermatozoa. Semen was diluted 1:1 with diluent A (BF5 + DMSO) at 25 degrees C and diluted further 1:1 with diluent B (diluent A + glycerol) after cooling down to 5 degrees C. Diluted semen was frozen immediately and stored in liquid nitrogen. Maximum percentages for motility and normal acrosomes were obtained in the presence of 12% DMSO (as expressed in diluent A) and 3% glycerol (final concentration) after thawing.
Bamba
K
K
AFRC, Institute of Animal Physiology, Cambridge, UK.
Adams
C E
CE
D000177:Acrosome / Q000187:drug effects; D000818:Animals; D015925:Cryopreservation; D003982:Dibekacin / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004338:Drug Combinations / Q000494:pharmacology; D005260:Female; D005990:Glycerol / Q000494:pharmacology; D008297:Male; D011817:Rabbits; D012662:Semen Preservation; D013081:Sperm Motility / Q000187:drug effects; D013451:Sulfonic Acids / Q000494:pharmacology; D014325:Tromethamine / Q000494:pharmacology
C064927:BF5 diluent; D004338:Drug Combinations; D013451:Sulfonic Acids; D014325:Tromethamine; D003982:Dibekacin; D005990:Glycerol; D004121:Dimethyl Sulfoxide