Survival of pig embryos flushed from the reproductive tract immediately or two hours after slaughter of donors.
Four- to eight-cell embryos were collected from genital tracts of slaughtered gilts. Prepuberal donors were synchronized and superovulated by PMSG and hCG, inseminated and eventually slaughtered approximately 78 h after the expected time of ovulation. Genital tracts were flushed either immediately after slaughter (group A) or after 2 h storage at either 37 degrees C (group B) or 20 degrees C). Best results were achieved in group A, followed by group B and, by a wide margin, group C. Differences in viability of embryos became more evident with longer culture times.
Wollenberg
C
C
University of Göttingen, Federal Republic of Germany.
Wentz
I
I
Blum
B
B
Holtz
W
W
D000818:Animals; D004624:Embryo Transfer / Q000662:veterinary; D005314:Embryonic and Fetal Development; D066298:In Vitro Techniques; D013552:Swine / Q000196:embryology*; D013997:Time Factors