High-performance liquid chromatographic determination of the mucoregulatory drug CO/1408 in rat plasma and urine.
A sensitive and selective high-performance liquid chromatographic (HPLC) method was developed for the determination of the mucoregulatory drug CO/1408 in plasma and urine. Samples containing an internal standard were prepared for analysis using a simple clean-up procedure based on Extrelut solid-phase extraction and chromatographed using a reversed-phase analytical column. Isocratic elution with a mobile phase consisting of 25 mM phosphate buffer (pH 2.5)-acetonitrile-methanol [85:10:5 or 87:9:4 (v/v) for plasma or urine analysis, respectively] was effected at a flow-rate of 0.8 ml/min. The eluate was monitored with an ultraviolet-visible variable-wavelength detector at 200 nm. The limit of quantification for the assay of CO/1408 was 80 ng/ml for plasma and 1 per 0.1 ml for urine samples. In spite of the high solubility of CO/1408 in water, the recovery from plasma and urine was very good and reproducible. The method was found to be applicable to pharmacokinetic studies of the drug in the rat.
Girometta
M A
MA
Department of Biochemistry and Pharmacokinetics, Camillo Corvi SpA, Piacenza, Italy.
Loschi
L
L
Ventura
P
P
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003511:Cyclohexanols / Q000097:blood*; D053138:Cyclohexenes; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D003511:Cyclohexanols; D053138:Cyclohexenes; C065237:6-hydroxy-4-(1-hydroxy-1-methylethyl)-1-cyclohexene-1-ethanol