Characterization of lignin peroxidase-encoding genes from lignin-degrading basidiomycetes.
Two closely linked lignin peroxidase (LPO)-encoding genes (lpo) from Phanerochaete chrysosporium were isolated. Nucleotide sequence studies indicated that the two genes are separated by 1.3 kb of flanking DNA and transcribed in opposite directions. Cloned P. chrysosporium lpo gene probes have been shown to hybridize to multiple sequences present in the DNAs of the white-rot fungi, Bjerkandera adusta, Coriolus versicolor and Fomes lignosus, but no hybridization was detected with DNA from Pleurotus ostreatus. Thus, lpo gene families appear to be common in a number of lignin-degrading basidiomycetes, some of which have not yet been shown to produce LPO proteins.
Huoponen
K
K
Department of Biochemistry, University of Turku, Finland.
Ollikka
P
P
Kälin
M
M
Walther
I
I
Mäntsälä
P
P
Reiser
J
J
D000595:Amino Acid Sequence; D001483:Base Sequence; D001487:Basidiomycota / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D005800:Genes, Fungal; D008040:Genetic Linkage; D007438:Introns; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010544:Peroxidases / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D004271:DNA, Fungal; D010544:Peroxidases; C042858:lignin peroxidase