title: Developmental changes in expression of contractile and cytoskeletal proteins in human aortic smooth muscle.
abstract: To describe phenotypic changes of human aortic smooth muscle cells (SMCs), proportion of smooth muscle and nonmuscle variants of actin, myosin heavy chains (MHCs), vinculin, and caldesmon, during prenatal and several months of postnatal development was determined. In aortic SMCs from 9-10-week-old fetus, both nonmuscle and smooth muscle-specific variants of all four proteins were present, however, the nonmuscle forms were more abundant. During development, a shift towards the expression of muscle-specific variants was observed, although the time course of changes in protein variant content was not similar for all the proteins studied. By the 24th week of gestation, fractional content of alpha-smooth muscle actin and smooth muscle MHCs was rather close to that in the mature SMCs, and comprised approximately 80 and 90%, respectively, of the levels characteristic of SMCs from adult aortic media. On the contrary, fractional ratio of meta-vinculin and 150-kDa caldesmon was still rather low in the aorta from the 24-week-old fetus, did not increase in a 2-month-old child aorta, and did not reach the level characteristic of mature SMCs even in the 6-month-old child aorta. Thus changes in alpha-smooth muscle actin and smooth muscle MHC fractional content occur mainly during the prenatal period of development, before the 24th week of gestation; while meta-vinculin and the 150-kDa caldesmon proportion increases mainly in the postnatal period, during several months after birth. In the "Discussion," phenotypes of SMCs from developing aorta were compared to those from different layers of the adult aortic wall.
authors.affiliation: Union of Soviet Socialist Republics Cardiology Research Center, Academy of Medical Sciences, Moscow.
authors.forename: M A
authors.initials: MA
authors.lastname: Glukhova
authors.forename: M G
authors.initials: MG
authors.lastname: Frid
authors.forename: V E
authors.initials: VE
authors.lastname: Koteliansky
mesh_terms: D000199:Actins / Q000378:metabolism; D000328:Adult; D000375:Aging; D001011:Aorta / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism; D003285:Contractile Proteins / Q000032:analysis / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000032:analysis / Q000378:metabolism*; D005314:Embryonic and Fetal Development; D005333:Fetus; D006801:Humans; D015151:Immunoblotting; D007223:Infant; D024510:Muscle Development; D009131:Muscle, Smooth, Vascular / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism
chemical_list: D000199:Actins; D003285:Contractile Proteins; D003598:Cytoskeletal Proteins