Preferential suppression of low molecular weight natural tumor suppressor of human fetal liver origin on the growth of leukemic cells in vitro.
A natural tumor suppressor of human fetal liver origin with low molecular weight (LMW-NTS) was studied. The methanol extract of human fetal liver supernatant (FLS-MeOH), a crude preparation of LMW-NTS, showed preferential suppression on the growth of leukemic cell lines HL-60, L833, WEHI-3, and L1210, but less effect on the growth of normal bone marrow granulocyte-macrophage colony-forming units (CFU-GM) in vitro. Preliminary experiments showed that FLS-MeOH also exhibited preferential suppression on the growth of leukemic colony-forming units (L-CFU) from six patients suffering from acute myeloid leukemia (AML). In combination with a long-term in vitro liquid culture system, FLS-MeOH could be adopted as an effective purging agent in the autologous bone marrow transplantation treatment of myeloid leukemia.
Pei
X T
XT
Department of Experimental Hematology, Beijing Institute of Radiation Medicine, China.
Wu
C T
CT
D000328:Adult; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002455:Cell Division / Q000187:drug effects; D003114:Colony-Forming Units Assay; D006098:Granulocytes / Q000166:cytology / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D007939:Leukemia L1210 / Q000473:pathology; D007941:Leukemia P388 / Q000473:pathology; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D008099:Liver / Q000196:embryology* / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D000432:Methanol; D051379:Mice; D008875:Middle Aged; D008970:Molecular Weight; D014411:Neoplastic Stem Cells / Q000473:pathology; D014182:Transplantation, Autologous; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D000432:Methanol