An improved procedure for the cultivation and in situ chromosome preparation of monolayer cell cultures.
A method for the cultivation of monolayer cell cultures on microslides in quadruple culture dishes together with a simple procedure for in situ chromosome preparation are described. The cells fixed to the slide can be stained according to standard procedures and analysed microscopically. The method is simple, rapid and reliable and provides many advantages especially for cytogenetic diagnostics with fibroblasts and amniotic fluid cells. It simplifies the performance of cytogenetic mutagenicity testing with primary cultures and permanent cell lines, e.g. the analysis of chromosome aberrations, sister chromatid exchanges (SCEs) and induced aneuploidy, as well as large-scale cytogenetic experiments.
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Abteilung Klinische Genetik, Universität Ulm, Federal Republic of Germany.
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D000818:Animals; D002478:Cells, Cultured; D002875:Chromosomes / Q000032:analysis*; D006224:Cricetinae; D046508:Culture Techniques / Q000379:methods*; D003582:Cytogenetics / Q000379:methods*