A study on the vital reaction in wounded skin: simultaneous determination of histamine and polyamines in injured rat skin by high-performance liquid chromatography.
This paper describes a modified method of simultaneous quantitative determination of histamine and polyamines (putrescine, cadaverine, spermidine and spermine) in incised skin wounds of rats using high-performance liquid chromatography (HPLC) with a strong-cation exchange column, shim-pack ISC-05/S0504P, employing a linear gradient of 0.7-2.5 M sodium chloride. There was scarcely any change in the histamine content of the vital wounds throughout the one week experimental period, although content decreased slightly in 1-h-old wounds. On the other hand, concentrations of putrescine and spermidine increased suddenly 12 h after wounding, and spermine content increased slightly in 4-day-old wounds. There was no post-mortem change in the histamine, spermidine and spermine contents of vital wounds allowed to stand at 25 degrees C up to 24 h after death. Based on these results, this HPLC method may be useful for the determination of wound age during the period of healing.
Maeno
Y
Y
Department of Legal Medicine, Nagoya City University Medical School, Japan.
Takabe
F
F
Inoue
H
H
Iwasa
M
M
D000818:Animals; D015317:Biogenic Polyamines / Q000378:metabolism*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006632:Histamine / Q000378:metabolism*; D008297:Male; D011180:Postmortem Changes; D011700:Putrescine / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000293:injuries / Q000378:metabolism*; D013095:Spermidine / Q000378:metabolism; D013096:Spermine / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology; D014950:Wounds, Penetrating / Q000378:metabolism*
D015317:Biogenic Polyamines; D013096:Spermine; D006632:Histamine; D013095:Spermidine; D011700:Putrescine