Molecular cloning of two species of cDNAs for human alpha-N-acetylgalactosaminidase and expression in mammalian cells.
Two species of cDNAs for human alpha-N-acetylgalactosaminidase were isolated from a human fibroblast cDNA library. The two species differ each other by a 70 bp insertion in the coding region. Transient expression study in COS cells demonstrated that only the cDNA without the 70 bp insertion expressed alpha-N-acetylgalactosaminidase activity. Analysis of mRNA species utilizing polymerase chain reaction revealed that the majority of the mRNA does not contain the 70 bp insertion, and the mRNA containing the 70 bp insertion is present only in a minor amount in human brain.
Yamauchi
T
T
Department of Neurology, Niigata University, Japan.
Hiraiwa
M
M
Kobayashi
H
H
Uda
Y
Y
Miyatake
T
T
Tsuji
S
S
D000118:Acetylglucosaminidase / Q000096:biosynthesis / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000032:analysis*; D006596:Hexosaminidases / Q000235:genetics*; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis*; D011994:Recombinant Proteins / Q000096:biosynthesis; D015183:Restriction Mapping
D004274:DNA, Recombinant; D007527:Isoenzymes; D012333:RNA, Messenger; D011994:Recombinant Proteins; D006596:Hexosaminidases; D000118:Acetylglucosaminidase