[Isolation of Ca 2+-activated photoprotein obelin from Obelia longissima and its use for the recording the outflow of Ca 2+ from fragmented sarcoplasmic reticulum of skeletal muscles].
A high-active stable preparation of obelin has been obtained from the luminescent hydroid Obelia longissima. The preparation is appropriate for determining free Ca2+ in the physiological range of its concentrations Obelin is shown possible to be used to record the processes of Ca2+ release from vesicles of the sarcoplasmic reticulum. In this case a rapid initial phase of Ca2+ outflux replaced by a slower one has been registered. A sharp increase of luminescence caused by the appearance of free Ca2+ in the medium has been registered under the effect of agents either increasing permeability of sarcoplasmic reticulum membranes for Ca2+ (A23187) or destroying the membrane (ethanol, triton X-100). The observed effects are confirmed, a radioactive label being used.
Gitel'zon
G I
GI
Tugaĭ
V A
VA
Zakharchenko
A N
AN
D000818:Animals; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D002463:Cell Membrane Permeability; D049449:Luminescence; D008164:Luminescent Proteins / Q000302:isolation & purification; D009132:Muscles / Q000378:metabolism*; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*
D008164:Luminescent Proteins; C010497:obelin; D002118:Calcium
Poluchenie Ca 2+-aktiviruemogo fotoproteina obelina iz Obelia longissima i ego primenenie dlia registratsii vykhoda Ca 2+ iz fragmentirovannogo sarkoplazmaticheskogo retikuluma skeletnykh myshts.