Effect of ornithine concentration on citrulline synthesis in mouse liver mitochondria.
The rate of citrulline synthesis in mitochondria from OTC-deficient spf-ash mice (15% of the normal activity) was found to be the same as that in mitochondria from control mice. The amount of NAG in their mitochondria varied markedly according to whether they had received a high- or low-protein diet, and the rate of citrulline synthesis was found to be affected by the level of NAG. These results indicate that the CPS stage, not the OTC stage, is rate-limiting in the citrulline synthesis process. Kinetic studies on the effect of ornithine concentration on citrulline synthesis in mitochondria showed that the Km for ornithine was very low in the mitochondria from the mice given a low-protein diet. Kinetic studies on the effect of ornithine concentration on mouse OTC at various concentrations of carbamylphosphate showed that OTC has a ping-pong mechanism, i.e., that the Km for ornithine and Vmax decrease with the reduction in carbamylphosphate concentration. This may explain the low Km value observed in citrulline synthesis in the mitochondria. We conclude that in mitochondrial citrulline synthesis the rate of carbamylphosphate synthesis by CPS in the presence of NAG plays a key role in determining the rate of citrulline synthesis and ornithine dependency.
Inoue
I
I
Department of Biochemistry, Faculty of Medicine, Kagoshima University.
Yoshimine
K
K
Saheki
T
T
D000818:Animals; D002956:Citrulline / Q000096:biosynthesis*; D005971:Glutamates / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008930:Mitochondria, Liver / Q000201:enzymology / Q000378:metabolism*; D009952:Ornithine / Q000378:metabolism*; D009954:Ornithine Carbamoyltransferase / Q000378:metabolism; D010101:Oxygen Consumption / Q000502:physiology
D005971:Glutamates; D002956:Citrulline; D009952:Ornithine; D009954:Ornithine Carbamoyltransferase; C016195:N-acetylglutamic acid