High-performance liquid chromatographic determination of the binding of ceftriaxone to human serum albumin solution and albumin from diluted human serum.
The binding of ceftriaxone to human serum albumin has been studied by high-performance liquid chromatography. The gel permeation method of Hummel and Dreyer was used. Ceftriaxone was tested with two sources of albumin (aqueous solution and diluted serum). After internal calibration the binding parameters were determined for each albumin, and results compared. These data are in agreement with those from classical methods for the determination of protein binding of ceftriaxone.
Mohler
J
J
Laboratoire de Biophysique, Faculté de Medecine Xavier Bichat, Paris, France.
Meulemans
A
A
Vulpillat
M
M
D002021:Buffers; D002443:Ceftriaxone / Q000032:analysis*; D002850:Chromatography, Gel; D006801:Humans; D008433:Mathematics; D012709:Serum Albumin / Q000032:analysis*; D012996:Solutions
D002021:Buffers; D012709:Serum Albumin; D012996:Solutions; D002443:Ceftriaxone