Antigenic assay for protein C determination in horses.
An antigenic assay was developed for determination of protein C in horses. Protein C, a natural, vitamin K-dependent anticoagulant component in blood, was isolated from equine plasma, a specific antibody was produced in goats, and a rocket electroimmunophoresis assay was established. Tests were performed to verify the identity of the isolated protein C and to determine the purity of the antibody. Protein C antigen was measured in plasma from 34 clinically normal horses, and values were compared with amidolytic function values. The mean (+/- SD) values for the 2 test methods were similar (antigen content, 104.5 +/- 13.8%; amidolytic activity, 104.6 +/- 17.5%), but the correlation coefficient was 0.1. Four horses given Na coumarin had markedly decreased plasma protein C amidolytic activity and minimal decrease in protein C antigen content.
Welles
E G
EG
Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens 30602.
Prasse
K W
KW
Duncan
A
A
Morris
M J
MJ
D000818:Animals; D000906:Antibodies; D000941:Antigens / Q000097:blood*; D006736:Horses / Q000097:blood*; D007122:Immunoelectrophoresis / Q000662:veterinary; D011486:Protein C / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification
D000906:Antibodies; D000941:Antigens; D011486:Protein C