title: Regulation of progesterone receptor gene expression.
abstract: The levels of progesterone receptor (PR) mRNA, PR protein, and [3H]R5020 binding activity were measured in parallel experiments conducted on a T47D subline expressing the estrogen receptor. A significant increase of PR mRNA levels could be detected within 6 h of exposure of the cells to estradiol (10(-8) M). The changes in mRNA, however, did not lead to any variation of PR protein levels of [3H]R5020 binding activity. A parallel analysis of PR mRNA and [3H]R5020 binding was then performed in a series of tumor biopsies. In estrogen receptor-positive and PR-positive tissues a correlation among the two values was found. It is postulated that the above mentioned data could reflect the existence of a difference in the mechanisms controlling the numerous steps of the PR synthesis in the various hormone-responsive tissues. This variability could allow an organ-specific response to the cyclic changes of circulating hormone.
authors.affiliation: Milano Molecular Pharmacology Laboratory, University of Milan, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Vegeto
authors.forename: M G
authors.initials: MG
authors.lastname: Cocciolo
authors.forename: F
authors.initials: F
authors.lastname: Raspagliesi
authors.forename: A
authors.initials: A
authors.lastname: Piffanelli
authors.forename: R
authors.initials: R
authors.lastname: Fontanelli
authors.forename: A
authors.initials: A
authors.lastname: Maggi
mesh_terms: D015152:Blotting, Northern; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007700:Kinetics; D009369:Neoplasms / Q000235:genetics* / Q000378:metabolism; D011397:Promegestone / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D011960:Receptors, Estrogen / Q000032:analysis; D011980:Receptors, Progesterone / Q000032:analysis / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D011397:Promegestone