Characterization of extracellular phospholipase A2 (PLA2) activity in fluid and peritoneal cells from casein-treated rats.
Extracellular phospholipase A2 activity (PLA2) found in the fluid and cells of the peritoneal cavity of rats injected with casein is described. PLA2 activities from both the fluid and cells require Ca2+ and have pH optima of 7. Acid-extraction increased PLA2 activity in the polymorphonuclear leukocyte (PMN) homogenates 20-fold but not the PLA2 activity in the extracellular fluid. Acid extraction also increased the sensitivity of the PLA2 activities to standard inhibitors. Since the PLA2 activities described in this model have characteristics similar to other inflammatory PLA2s, including human synovial fluid PLA2, casein stimulation should prove useful for testing potential inhibitors.
Stevens
T M
TM
Medical Products Department, E.I. du Pont de Nemours & Co., Inc., Wilmington, Delaware 19880-0400.
McGowan
M
M
Giannaras
J
J
Kerr
J S
JS
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D002364:Caseins / Q000494:pharmacology*; D004195:Disease Models, Animal; D005110:Extracellular Space / Q000201:enzymology*; D005122:Exudates and Transudates / Q000201:enzymology; D007249:Inflammation / Q000201:enzymology; D008297:Male; D010529:Peritoneal Cavity / Q000166:cytology* / Q000502:physiology; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000378:metabolism*; D054467:Phospholipases A2; D051381:Rats; D011919:Rats, Inbred Strains
D000893:Anti-Inflammatory Agents; D002364:Caseins; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2