title: Studies on glycosphingolipids in larvae of the green-bottle fly, Lucilia caesar: two neutral glycosphingolipids having large straight oligosaccharide chains with eight and nine sugars.
abstract: Two neutral glycosphingolipids having large straight oligosaccharide chains with eight and nine sugars, provisionally named COS and CNS, were isolated and purified from larvae of the green-bottle fly, Lucilia caesar, as the only two remaining unidentified significant neutral glycolipids in this organism. From the results of sugar analysis, permethylation, negative-ion fast atom bombardment mass spectroscopy (FAB-MS), and 1H-NMR studies, the structures of the two glycolipids are proposed to be: COS, GalNAc beta 1-3GlcNAc beta 1-3Gal beta 1-3GalNAc alpha 1-4GalNAc beta 1-4GlcNAc beta 1-3Man beta 1-4Glc beta 1-Cer; and CNS, Gal beta 1-3GalNAc beta 1-3GlcNAc beta 1-3Gal beta 1-3GalNAc alpha 1-4GalNAc beta 1-4GlcNAc beta 1-3Man beta 1-4Glc beta 1-Cer. The fatty acid and long-chain base compositions of the above glycolipids were very similar, and were dominated by arachidic acid, and tetradeca- and hexadeca-4-sphingenines. The great similarity between the compositions of their ceramide moieties suggests that COS may be a precursor in the glycosylation reaction yielding CNS.
authors.affiliation: Department of Chemistry, Faculty of Liberal Arts and Education, Shiga University.
authors.forename: M
authors.initials: M
authors.lastname: Sugita
authors.forename: F
authors.initials: F
authors.lastname: Inagaki
authors.forename: H
authors.initials: H
authors.lastname: Naito
authors.forename: T
authors.initials: T
authors.lastname: Hori
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D006028:Glycosphingolipids / Q000032:analysis*; D007814:Larva / Q000032:analysis; D008969:Molecular Sequence Data; D009117:Muscidae / Q000032:analysis*; D020383:Neutral Glycosphingolipids; D009844:Oligosaccharides / Q000032:analysis*
chemical_list: D006028:Glycosphingolipids; D020383:Neutral Glycosphingolipids; D009844:Oligosaccharides; C065513:glycosphingolipid COS; C065514:glycosphingolipid CNS