Characterization of adrenoceptor subtypes in cat cutaneous vasculature.
Experiments were undertaken to characterize the relative contribution of adrenoceptor subtypes in mediating vasoconstriction to exogenous agonists in the digital cutaneous vascular bed of the anesthetized cat using laser-Doppler flowmetry. Intra-arterial administration of (-)-epinephrine and (-)-norepinephrine into the brachial artery caused a dose-related vasoconstriction (decreased flow) with ED50 values of 7 and 21 ng, respectively. Blockade of beta adrenoceptors with propranolol did not alter the response to (-)-epinephrine nor did i.a. isoproterenol produce a significant vasodilation. Vasoconstrictor responses elicited by (-)-epinephrine and (-)-norepinephrine were antagonized by treatment with phentolamine (2.5 mg/kg i.v.) and by yohimbine (0.5 mg/kg i.v.) but were only marginally blocked by prazosin (0.1 mg/kg i.v.). A dose-related depression of cutaneous blood flow was also caused by clonidine at doses virtually identical to those of (-)-norepinephrine. Clonidine-induced vasoconstriction was antagonized by rauwolscine (0.5 mg/kg i.v.) but not by prazosin (0.1 mg/kg i.v.). Dose-response curves to a variety of additional adrenoceptor stimulants were constructed with the potency rank order for all agonists being: (-)-epinephrine greater than B-HT 920 = (-)-norepinephrine = clonidine much greater than (-)-phenylephrine much greater than B-HT 933 greater than methoxamine. Treatment with prazosin (0.1 mg/kg i.v.) antagonized methoxamine induced cutaneous vasoconstriction but not the decreased blood flow caused by B-HT 933. In contrast, rauwolscine (0.5 mg/kg i.v.) blocked the responses to B-HT 933 but not methoxamine.(ABSTRACT TRUNCATED AT 250 WORDS)
Koss
M C
MC
Department of Pharmacology, University of Oklahoma Health Sciences Center, Oklahoma City.
D000818:Animals; D001381:Azepines / Q000494:pharmacology; D002415:Cats; D003000:Clonidine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004837:Epinephrine / Q000494:pharmacology; D005260:Female; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D011224:Prazosin / Q000494:pharmacology; D011941:Receptors, Adrenergic / Q000032:analysis* / Q000187:drug effects; D012039:Regional Blood Flow / Q000187:drug effects; D012867:Skin / Q000098:blood supply*; D014661:Vasoconstriction / Q000187:drug effects
D001381:Azepines; D011941:Receptors, Adrenergic; C015603:azepexole; D003000:Clonidine; D009638:Norepinephrine; D011224:Prazosin; D004837:Epinephrine