Complementary DNA sequence of 3-methylcholanthrene-inducible P-450 from the rat lung.
Cytochrome P-450MC was induced in pulmonary microsomes of 3-methylcholanthrene-treated rats and also at low level in that of isosafrole-treated rats. Cytochrome P-450d was not detected in the lungs of 3-methylcholanthrene- or isosafrole-treated rats by the method of Western blot analysis with a polyclonal antibody raised against cytochrome P-450c which is equally effective against P-450d, nor by the method of Northern hybridization probed with pcP450mc3 (P-450d probe). Complementary DNA of P-450MC was isolated from rat pulmonary cDNA library and the nucleotide sequence of pulmonary cDNA was compared with that of hepatic P-450c cDNA reported by Yabusaki et al. There was no gross change in nucleotide sequences of cDNA between pulmonary P-450MC and hepatic P-450c.
Kikuchi
H
H
Department of Cancer Chemotherapy and Prevention, Tohoku University, Sendai, Japan.
Sagami
I
I
Fujii
H
H
Ohmachi
T
T
Watanabe
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000032:analysis*; D008099:Liver / Q000201:enzymology*; D008168:Lung / Q000201:enzymology*; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D051381:Rats; D011915:Rats, Inbred BUF
D008748:Methylcholanthrene; D004247:DNA; D003577:Cytochrome P-450 Enzyme System