title: Prep-A-Gene: a superior matrix for the purification of DNA and DNA fragments.
abstract: A new chromatographic matrix, Prep-A-Gene, is described for the isolation and purification of high quality DNA suitable for restriction analysis, ligation, transformation and sequencing protocols. This matrix selectively binds DNA greater than approximately 200 base pairs in length, while RNA, proteins, cellular components, agarose and other contaminants are washed free in minutes. This eliminates the need for time-consuming and laborious RNase treatments, gel extractions and phenol extractions. The DNA that is desorbed from the matrix is available immediately as a substrate for subsequent protocols. DNA purified in this manner exhibits no detectable shearing, even with more fragile chromosomal DNA.
authors.affiliation: Bio-Rad Laboratories, Richmond, CA 94804.
authors.forename: E H
authors.initials: EH
authors.lastname: Willis
authors.forename: E R
authors.initials: ER
authors.lastname: Mardis
authors.forename: W L
authors.initials: WL
authors.lastname: Jones
authors.forename: M C
authors.initials: MC
authors.lastname: Little
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D002853:Chromatography, Liquid / Q000379:methods*; D004247:DNA / Q000302:isolation & purification*; D004274:DNA, Recombinant / Q000302:isolation & purification; D004277:DNA, Single-Stranded / Q000302:isolation & purification; D004279:DNA, Viral / Q000302:isolation & purification; D051379:Mice; D008969:Molecular Sequence Data
chemical_list: D004274:DNA, Recombinant; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004247:DNA