Inhibition of lipolysis by hydrocarbons and fatty alcohols.
The hydrolysis of long-chain triglyceride by pancreatic lipase (EC 3.1.1.3) is inhibited by hydrophobic solutes that are dissolved in the fat. Solutes tested included n-alkanes (C10-C16), aromatic and chlorinated aromatic hydrocarbons (including a PCB and DDT), n-alcohols (C10-C16), and cholesterol. Except for cholesterol, which stimulated lipolysis at low concentrations, all compounds produced roughly similar inhibition curves that followed the pattern of a typical Langmuir adsorption isotherm (Mattson, F. H., R. A. Volpenhein, and L. Benjamin, 1970. J. Biol. Chem. 245: 5335-5340). According to this interpretation, hydrophobic solutes dissolved within fat droplets partition between the interior oil phase and the surface monolayer where lipolysis occurs. Although the aromatic and chlorinated aromatic hydrocarbons were approximately 25% more inhibitory than the long-chain aliphatic hydrocarbons, as a single class, hydrocarbons were 7-10 times weaker inhibitors of lipolysis than fatty alcohols. In contrast to the alcohols whose inhibitory action may involve several mechanisms, the hydrocarbons behaved like simple dilution inhibitors; i.e., at 50% inhibition the mass ratio of hexadecane to triglyceride was 0.42. The lack of a chain length effect indicates that the hydrocarbons are not adsorbed at the interface but interdigitate the triglyceride molecules and align parallel to the lipid acyl chains. Inhibition by hydrophobic solutes was not reversed by the presence of 4 mM taurodeoxycholate and pancreatic procolipase or colipase.
Ferreira
G C
GC
Department of Biological Chemistry, School of Medicine, Johns Hopkins University, Baltimore, MD 21205.
Patton
J S
JS
D000818:Animals; D004655:Emulsions; D005233:Fatty Alcohols / Q000494:pharmacology*; D006838:Hydrocarbons / Q000494:pharmacology*; D066298:In Vitro Techniques; D008049:Lipase / Q000378:metabolism; D008066:Lipolysis / Q000187:drug effects*; D010179:Pancreas / Q000201:enzymology; D013552:Swine
D004655:Emulsions; D005233:Fatty Alcohols; D006838:Hydrocarbons; D008049:Lipase