Effects of lysostaphin on Staphylococcus aureus infections of the mouse mammary gland.
A 5 to 6 log10 reduction in the viable count of Staphylococcus aureus was produced in vitro with 10 micrograms lysostaphin ml-1 milk. Infusion of the lactating murine mammary gland with 10 micrograms lysostaphin, immediately following inoculation with 10(8) colony forming units of S aureus, resulted in a significant 2 to 3 log10 reduction in viable S aureus (P less than 0.02) within 30 minutes. Pre-infusion with 10 micrograms lysostaphin either immediately before or one hour before staphylococcal challenge reduced the recovery of S aureus by more than 6 log10 and greatly reduced pathological changes typical of S aureus mastitis. This clearly demonstrates that lysostaphin has considerable potential for the therapeutic or prophylactic control of staphylococcal mastitis.
Bramley
A J
AJ
AFRC Institute for Animal Health, Compton Laboratory, Newbury, Berkshire.
Foster
R
R
D000818:Animals; D002417:Cattle; D004195:Disease Models, Animal; D008248:Lysostaphin / Q000627:therapeutic use*; D008321:Mammary Glands, Animal / Q000382:microbiology; D008414:Mastitis, Bovine / Q000188:drug therapy*; D051379:Mice; D013203:Staphylococcal Infections / Q000188:drug therapy*; D013211:Staphylococcus aureus / Q000187:drug effects
D008248:Lysostaphin