Fasting prevents acute pancreatitis induced by cerulein in rats.
We examined the effect of fasting on the course of experimental acute pancreatitis induced in rats by four subcutaneous injections of 20 micrograms/kg body weight of cerulein at hourly intervals. Rats were either fasted from 24 hr before to 9 hr after the first cerulein injection or fed ad libitum throughout the experiment. Twenty-four hours of fasting reduced cerulein-induced increases in serum levels of amylase and anionic trypsin(ogen) to 50 and 70% of those in fed rats, respectively. Increases in pancreatic wet weight after cerulein injections were also less in fasted rats than in fed rats. Pancreatic content of trypsin was significantly decreased after a 24-hr fast, and no further changes were induced by cerulein injections. The histological signs of acute pancreatitis were greatly alleviated by fasting. However, 24 hr of fasting did not alter the sensitivity and responsiveness of the exocrine pancreas to cerulein in both in vivo and in vitro. Plasma CCK bioactivity and immunoreactive secretin concentration in 24-hr-fasted rats were significantly lower than those in fed rats. Administration of CCK receptor antagonist, loxiglumide, 12 hr prior to the induction of acute pancreatitis reduced the increase in serum amylase activity in fed rats to nearly the same levels as that in fasted rats and alleviated histological signs of pancreatitis to some extent. These present observations suggest that fasting lessens the severity of cerulein-induced acute pancreatitis by reducing endogenous CCK release.
Otsuki
M
M
Second Department of Internal Medicine, Kobe University School of Medicine, Japan.
Tani
S
S
Okabayashi
Y
Y
Fujii
M
M
Nakamura
T
T
Fujisawa
T
T
Koide
M
M
Itoh
H
H
D000208:Acute Disease; D000818:Animals; D002108:Ceruletide / Q000633:toxicity*; D002766:Cholecystokinin / Q000037:antagonists & inhibitors / Q000097:blood; D005215:Fasting; D008297:Male; D010179:Pancreas / Q000473:pathology; D010195:Pancreatitis / Q000139:chemically induced* / Q000473:pathology; D011377:Proglumide / Q000031:analogs & derivatives / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012633:Secretin / Q000097:blood
D012633:Secretin; C053737:loxiglumide; D002108:Ceruletide; D002766:Cholecystokinin; D011377:Proglumide