Studies of the immunohistochemical and biochemical localization of the cytochrome P-450scc-linked monooxygenase system in the adult rat brain.
Immunohistochemical and biochemical studies were performed on the brains of adult female and male rats using a specific antibody against bovine adrenocortical cytochrome P-450scc. The results showed that in both male and female rats, the myelinated regions of the white matter are selectively immunostained throughout the brain and that even in rats pretreated with colchicine, there is never positive staining of neuronal cell bodies and their dendrites in any brain region. Western immunoblotting with the P-450scc antibody and enzymatic assays revealed that P-450scc and cholesterol side-chain cleavage activity were present in a homogenate derived from the cortical white matter, but not detectable in that from the cerebral cortex. Furthermore, quantitation of the P-450scc protein in the immunoblots indicated that the concentration of P-450scc in the cortical white matter of both female and male rat brains is approx. 3-4 pmol per mg tissue protein. Thus it could be concluded that in the adult rat brain, P-450scc and cholesterol side-chain cleavage activity are selectively localized only in the myelinated region of the white matter.
Iwahashi
K
K
Department of Biochemistry, Faculty of Medicine, Kagawa Medical School, Japan.
Ozaki
H S
HS
Tsubaki
M
M
Ohnishi
J
J
Takeuchi
Y
Y
Ichikawa
Y
Y
D000302:Adrenal Cortex / Q000201:enzymology; D000818:Animals; D000936:Antigen-Antibody Complex; D015153:Blotting, Western; D001921:Brain / Q000166:cytology / Q000201:enzymology*; D002417:Cattle; D002540:Cerebral Cortex / Q000201:enzymology; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000032:analysis* / Q000378:metabolism; D005260:Female; D007150:Immunohistochemistry; D007700:Kinetics; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains
D000936:Antigen-Antibody Complex; D002786:Cholesterol Side-Chain Cleavage Enzyme