Detection and characterization of mumps virus V protein.
By cDNA cloning, DNA sequencing, and RNAse mapping analyses two distinct mRNA species were shown to be transcribed from the mumps virus P gene; one faithful and the other edited copies. The former was found to direct the synthesis of the V protein (25K), while the latter, after the addition of two nontemplated G residues, was found to direct the synthesis of the P protein (40K-42K). The carboxy terminal of the V protein contained a cysteine-rich region which was similar but not identical to the metal binding domain motif found in several nucleic acid binding proteins. The V protein was detected not only in mumps virus-infected cells but also in the virions by antiserum raised against a synthetic peptide.
Takeuchi
K
K
Department of Measles Virus, National Institute of Health, Tokyo, Japan.
Tanabayashi
K
K
Hishiyama
M
M
Yamada
Y K
YK
Yamada
A
A
Sugiura
A
A
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D001483:Base Sequence; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009109:Mumps virus / Q000235:genetics*; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000096:biosynthesis*; D012260:Ribonucleases; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology; D014771:Virion / Q000235:genetics
D000914:Antibodies, Viral; D004279:DNA, Viral; D012333:RNA, Messenger; D014764:Viral Proteins; D012260:Ribonucleases