title: The maturation-dependent conformational change of the major capsid protein of bacteriophage T4 involves a substantial change in secondary structure.
abstract: We have investigated the conformational basis of the expansion transformation that occurs upon maturation of the bacteriophage T4 prohead, by using laser Raman spectroscopy to determine the secondary structure of the major capsid protein in both the precursor and the mature states of the surface lattice. This transformation involves major changes in the physical, chemical, and immunological properties of the capsid and is preceded in vivo by processing of its major protein, gp23 (56 kDa), to gp23* (49 kDa), by proteolysis of its N-terminal gp23-delta domain. The respective secondary structures of gp23 in the unexpanded state, and of gp23* in the expanded state, were determined from the laser Raman spectra of polyheads, tubular polymorphic variants of the capsid. Similar measurements were also made on uncleaved polyheads that had been expanded in vitro and, for reference, on thermally denatured polyheads. We find that, with or without cleavage of gp23, expansion is accompanied by substantial changes in secondary structure, involving a major reduction in alpha-helix content and an increase in beta-sheet. The beta-sheet contents of gp23* or gp23 in the expanded state of the surface lattice, and even of gp23 in the unexpanded state, are sufficient for a domain with the "jellyroll" fold of antiparallel beta-sheets, previously detected in the capsid proteins of other icosahedral viruses.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Laboratory of Physical Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, Bethesda, Maryland 20892.
authors.forename: A C
authors.initials: AC
authors.lastname: Steven
authors.forename: H
authors.initials: H
authors.lastname: Greenstone
authors.forename: A C
authors.initials: AC
authors.lastname: Bauer
authors.forename: R W
authors.initials: RW
authors.lastname: Williams
mesh_terms: D002213:Capsid / Q000648:ultrastructure*; D008854:Microscopy, Electron; D010942:Plant Viruses / Q000648:ultrastructure; D011487:Protein Conformation; D011489:Protein Denaturation; D013059:Spectrum Analysis, Raman; D013604:T-Phages / Q000254:growth & development / Q000648:ultrastructure*