Effects of transforming growth factor-beta and epidermal growth factor on clonal rat pulp cells.
These factors influence proliferation and differentiation in various cell types. Their effects on a clonal cell line (RPC-C2A) having high ALPase activity were examined by assay of [3H]-thymidine incorporation and ALPase activity. Neither factor (at a dose of 0.5 ng/ml) altered the shape of the pulp cells. DNA synthesis was not affected by transforming growth factor-beta either in growing cells or in those nearly confluent, but epidermal growth factor, in doses ranging from 0.5 to 10 ng/ml, stimulated the incorporation of [3H]-thymidine in nearly confluent cells. Both factors inhibited ALPase activity in a dose-dependent manner. Indomethacin did not affect this inhibition, suggesting that this effect of growth factors may not be mediated by prostaglandin synthesis. Inhibitory effects of ALPase antagonists (L-phenylalanine, L-homoarginine, levamisole) were not affected by transforming growth factor-beta. Thus epidermal growth factor stimulates DNA synthesis and both transforming growth factor-beta and epidermal growth factor inhibit ALPase activity of clonal rat pulp cells, suggesting that both factors may act as regulators of biological function, including cell differentiation, in pulp cells.
Liang
R F
RF
Department of Pharmacology, School of Dentistry, Meikai University, Saitama, Japan.
Nishimura
S
S
Maruyama
S
S
Hanazawa
S
S
Kitano
S
S
Sato
S
S
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000378:metabolism; D000818:Animals; D002999:Clone Cells; D004247:DNA / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D015291:Transforming Growth Factors / Q000494:pharmacology*
D004815:Epidermal Growth Factor; D015291:Transforming Growth Factors; D004247:DNA; D000469:Alkaline Phosphatase