title: In situ (6-4)photoproduct determination by laser cytometry and autoradiography.
abstract: The UV-induced (6-4)photoproducts and their repair in individual human cells were quantitatively determined by argon-laser imaging microspectrofluorometry or autoradiography with a well-characterized monoclonal antibody against (6-4)photoproducts. (6-4)Photoproduct induction curves were linear as a function of UV dose, using both methods. The formation of (6-4)photoproducts was detected in the cells irradiated with as low as 10 and 25 J/m2 of UV by autoradiography and laser cytometry, respectively. Normal cells repaired more than 80% of the initial damage within 4 h post irradiation. In contrast, almost no repair was observed in xeroderma pigmentosum cells (complementation group A) within 8 h.
authors.affiliation: Division of Radiation Biology, Faculty of Pharmaceutical Sciences, Kanazawa University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Mori
authors.forename: T
authors.initials: T
authors.lastname: Matsunaga
authors.forename: C C
authors.initials: CC
authors.lastname: Chang
authors.forename: J E
authors.initials: JE
authors.lastname: Trosko
authors.forename: O
authors.initials: O
authors.lastname: Nikaido
mesh_terms: D000911:Antibodies, Monoclonal; D001345:Autoradiography / Q000379:methods; D002478:Cells, Cultured; D004249:DNA Damage; D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000166:cytology / Q000528:radiation effects; D006801:Humans; D007700:Kinetics; D007834:Lasers; D008856:Microscopy, Fluorescence / Q000379:methods; D013997:Time Factors; D014466:Ultraviolet Rays; D014983:Xeroderma Pigmentosum
chemical_list: D000911:Antibodies, Monoclonal