Immunochemical cross-reactivity between albumin and solid-phase adsorbed histamine.
For production of an antibody against histamine, this was coupled to human serum albumin (HSA) and used for immunization of rabbits. To test the antiserum, an immunoradiometric assay was developed comprising solid-phase bound histamine, antisera and radiolabelled protein A. Titration and inhibition experiments revealed that histamine adsorbed onto a solid-phase could bind the antiserum. However, neither free histamine nor histamine coupled to unrelated carriers could inhibit the binding of antiserum to the solid-phase histamine. Cross-reactivity was demonstrated between HSA and solid-phase bound histamine, as the immunoradiometric assay was inhibited by HSA. This unexpected cross-reactivity was established, as a commercially available antiserum with specificity to HSA without histamine also bound to the solid-phase bound histamine. It is suggested that preparations of antibodies against histamine are tested for this possible cross-reactivity.
Poulsen
L K
LK
Laboratory of Medical Allergology, State University Hospital, Copenhagen, Denmark.
Nolte
H
H
Søndergaard
I
I
Stahl Skov
P
P
D000327:Adsorption; D000818:Animals; D000918:Antibody Specificity; D003429:Cross Reactions / Q000276:immunology; D006632:Histamine / Q000276:immunology*; D006801:Humans; D007114:Immunization; D015592:Immunoradiometric Assay; D007164:Immunosorbents; D007457:Iodine Radioisotopes; D011817:Rabbits; D012709:Serum Albumin / Q000276:immunology*; D013205:Staphylococcal Protein A
D007164:Immunosorbents; D007457:Iodine Radioisotopes; D012709:Serum Albumin; D013205:Staphylococcal Protein A; D006632:Histamine