[A mechanism of luminol-dependent chemiluminescence of human serum in the presence of hydrogen peroxide].
Comparative role of proteins of the human blood plasma in luminol-dependent induction of chemiluminescence in the presence of hydrogen peroxide was studied. It was found that the largest contribution into chemiluminescence was made by the plasma fraction with the molecular mass 250 kD. Besides the intensity of chemiluminescence proves to be proportional to hemoglobin concentration, which when added to the blood plasma entered the fractions with the molecular mass about 250 kD. It is suggested that hemoglobin producing luminescence in the blood plasma is related to haptoglobin.
Kozlov
A V
AV
Osipov
A N
AN
Vladimirov
Iu A
IuA
D001769:Blood; D001798:Blood Proteins / Q000378:metabolism; D005609:Free Radicals; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology*; D007700:Kinetics; D049449:Luminescence; D008165:Luminol / Q000494:pharmacology*; D011724:Pyridazines / Q000494:pharmacology*
D001798:Blood Proteins; D005609:Free Radicals; D006454:Hemoglobins; D011724:Pyridazines; D008165:Luminol; D006861:Hydrogen Peroxide
Mekhanizm liuminolzavisimoĭ khemiliuminestsentsii syvorotki krovi cheloveka v prisutstvii peroksida vodoroda.