Uncoupling of DNA excision repair and nucleosomal unfolding in poly(ADP-ribose)-depleted mammalian cells.
The repair of DNA damage in eukaryotic cells is closely coupled with local changes of chromatin structure such that newly synthesized repair patches transiently appear in 'free' DNA domains with increased accessibility to enzymatic and chemical probes. We have isolated these domains from mammalian cells repairing bulky DNA adducts. During the first 3 h of repair, excision of adducts occurred exclusively in free DNA and was closely linked with the appearance of newly synthesized repair patches. Following depletion of chromatin-bound poly(ADP-ribose), the repositioning of repair patches into these domains was completely blocked, although overall repair patch synthesis was unaltered. Concomitantly, DNA adducts were no longer excised and tended to accumulate in free DNA domains. Our results suggest a tight coupling of the excision step with the formation of free DNA domains by a mechanism involving poly ADP-ribosylation of chromatin proteins.
Mathis
G
G
University of Zürich (Tierspital), Institute of Pharmacology and Biochemistry, Switzerland.
Althaus
F R
FR
D000099:Acetoxyacetylaminofluorene / Q000633:toxicity; D000818:Animals; D002843:Chromatin / Q000378:metabolism*; D004247:DNA / Q000187:drug effects / Q000378:metabolism*; D004249:DNA Damage; D004260:DNA Repair; D008730:Methoxsalen / Q000378:metabolism; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D011064:Poly Adenosine Diphosphate Ribose / Q000172:deficiency / Q000378:metabolism*; D051381:Rats; D013997:Time Factors
D002843:Chromatin; D009702:Nucleoside Diphosphate Sugars; D011064:Poly Adenosine Diphosphate Ribose; D000099:Acetoxyacetylaminofluorene; D004247:DNA; D008730:Methoxsalen