title: Dimethylsulphoxide affects the organisation of microfilaments in the mouse oocyte.
abstract: The effect of dimethylsulphoxide (DMSO) on microfilament organisation has been studied in the mouse oocyte after staining with (NBD)-phallacidin. The cortical actin meshwork was disrupted by exposure of oocytes to 1.5 M DMSO at 37 degrees C, and this disruption was associated with changes in the cell surface, especially microvilli length and distribution, as observed by scanning electron microscopy. The irregular distribution of actin filaments observed also appears to lead to an irregular expansion of the cell after DMSO removal. However, when exposure to DMSO was combined with cooling, the effects on the microfilament system were much reduced. The reversibility of DMSO action is considered and the potential implications of microfilament disruption on the viability and functions of the oocyte discussed.
authors.affiliation: Department of Anatomy, University of Cambridge, England.
authors.forename: C
authors.initials: C
authors.lastname: Vincent
authors.forename: S J
authors.initials: SJ
authors.lastname: Pickering
authors.forename: M H
authors.initials: MH
authors.lastname: Johnson
authors.forename: S J
authors.initials: SJ
authors.lastname: Quick
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D003451:Cryoprotective Agents / Q000494:pharmacology*; D003599:Cytoskeleton / Q000502:physiology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005260:Female; D005307:Fertilization in Vitro; D005615:Freezing; D051379:Mice; D008815:Mice, Inbred Strains; D008855:Microscopy, Electron, Scanning; D009865:Oocytes / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide