title: Histochemical study of matrical components in the mandibular condyle of the neonatal mouse.
abstract: In the mandibular condyle of the newborn mouse the chondroprogenitor (CP) zone is the only layer that incorporates 3H-thymidine thus serving the source for cells of the cartilage lineage. Ultrastructurally these cells have a mesenchymal appearance surrounded by collagen fibrils as well as by additional filaments that become apparent following fixation with ferrocyanide-reduced OsO4. In addition, electron-dense particles indicative of proteoglycans are scattered throughout the matrix in the CP zone as well as in the chondroblastic and hypertrophic zones. Following labeling with 35S-sulfate the CP zone as well as the other compartments revealed a substantial number of grains following processing for autoradiography. The number of grains per cell was highest in the hypertrophic zone. Indirect immunofluorescence indicated the presence of fibronectin in the articular surface, CP zone and in the hypertrophic zone. The immunogold method localized fibronectin intracellularly in CP cells and extracellularly in the hypertrophic zone. Therefore, in the mandibular condyle the CP cells which are capable for DNA synthesis are also involved in the synthesis of macromolecules of which some are specific for the cartilage phenotype, while others are associated with other functions of connective tissue cells.
authors.affiliation: Rappaport Family Institute for Research in the Medical Sciences, Faculty of Medicine, Technion-Israel Institute of Technology, Haifa.
authors.forename: E
authors.initials: E
authors.lastname: Livne
authors.forename: D
authors.initials: D
authors.lastname: Lewinson
authors.forename: G
authors.initials: G
authors.lastname: Dickson
authors.forename: M
authors.initials: M
authors.lastname: Silbermann
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000378:metabolism*; D001345:Autoradiography; D005109:Extracellular Matrix / Q000378:metabolism* / Q000648:ultrastructure; D007150:Immunohistochemistry; D008335:Mandibular Condyle / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D051379:Mice; D008813:Mice, Inbred ICR; D008854:Microscopy, Electron