Changes in the DNA ploidy patterns of human colorectal carcinomas, subsequent to culture or xenografting.
DNA ploidy patterns were measured by flow cytometry in 7 human carcinomas and compared with the DNA histograms of 3 cell lines and 25 xenografts and derived from them. Four of the carcinomas showed DNA ploidy changes on initial xenografting or early passaging, while 2 carcinomas showed stable ploidy patterns over several xenograft passages. One xenograft derived from a cell line showed a marked, but stable, increase in DNA content over the original human carcinoma. Two of the 3 cell lines were DNA aneuploid, and at least 1 line was apparently derived from a minor subpopulation in the original carcinoma. The possible mechanisms and implications of these changes are discussed.
Foster
H M
HM
Department of Surgical Oncology, Newcastle Mater Misericordiae Hospital, Waratah, NSW, Australia.
Tay
D L
DL
Whitehead
R H
RH
D000818:Animals; D015179:Colorectal Neoplasms / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics*; D005434:Flow Cytometry; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D011003:Ploidies; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm