Homogeneous latex immunoassay for thyroid hormone testing. Determination of thyroxine and triiodothyronine.
We describe here two latex immunoassays for total thyroxine (T4) and total triiodothyronine (T3). These homogeneous 60 min assays are quantified by optically counting the monomeric particles remaining after agglutination. When precision is assessed, both methods display coefficients of variation of 3-7% for within-run assays and 4-10% for between-run assays. The accuracy of the methods, as tested by dilution and spike recovery experiments, was found to be satisfactory. Two correlation studies were carried out to compare the present method with leading commercial methods. The coefficients obtained were: r = 0.92 and r = 0.93 with 150 sera for T3, and r = 0.95 (150 sera) and r = 0.93 (108 sera) for T4.
Mareschal
J C
JC
Unit of Experimental Medicine, Faculty of Medicine, Catholic University of Louvain, Brussels, Belgium.
Cambiaso
C L
CL
Janssens
J
J
Limet
J N
JN
Tasiaux
N
N
Collet-Cassart
D
D
D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007841:Latex Fixation Tests; D013974:Thyroxine / Q000032:analysis*; D014284:Triiodothyronine / Q000032:analysis*
D014284:Triiodothyronine; D013974:Thyroxine