Computer aided time-lapse video analysis of hepatocyte morphology during adhesion to cellulose membranes.
An investigation was performed to demonstrate that time-lapse cinematography and computer aided video analysis of cell morphology is suitable to study and compare the characteristics of hepatocytes during the adhesion process to membranes. We chose to compare ordinary cellulose Cuprophan membranes and membranes coated with collagen or fibronectin. Striking differences between uncoated cellulose and fibronectin or collagen coating were seen in the cell count per square millimeter and adhesion behaviour. On the investigated uncoated Cuprophan the hepatocytes were found to attach but not to spread whilst on collagen coated Cuprophan most of the cells spread spherically, and on fibronectin coated membranes most of the cells flattened spherically or polygonally. Time-lapse video microscopy seems to be a valuable technique for assessing the morphologic behaviour of cells in a detailed and quantitative manner in order to improve the hepatocyte culture technique in bioreactors for hybrid systems.
Gerlach
J
J
Chirurgische Klinik, Freie Universität Berlin, F.R.G.
Vienken
J
J
Walker
P
P
Affeld
K
K
D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion; D002482:Cellulose / Q000031:analogs & derivatives; D003094:Collagen; D005353:Fibronectins; D007091:Image Processing, Computer-Assisted; D008099:Liver / Q000166:cytology*; D008297:Male; D008567:Membranes, Artificial; D051381:Rats; D011919:Rats, Inbred Strains; D014743:Videotape Recording
D001672:Biocompatible Materials; D005353:Fibronectins; D008567:Membranes, Artificial; D002482:Cellulose; D003094:Collagen; C009865:cuprammonium cellulose