title: [Chemical modification of cholesterol-hydroxylating cytochrome P-450 from adrenal cortex mitochondria with diethylpyrocarbonate].
abstract: Chemical modification of adrenocortical cytochrome P-450scc with diethyl pyrocarbonate has been carried out. The histidine residues and the protein amino groups were shown to undergo modification. Carbethoxylation was accompanied by the hemoprotein inactivation and the loss of enzymatic activity. Neither of the high spin effectors (i.e., substrate and adrenodoxin) protected cytochrome P-450scc either from inactivation or from the loss of enzymatic activity. The data obtained are discussed in terms of the functional role of histidine residues in the cytochrome P-450scc molecule.
authors.forename: I A
authors.initials: IA
authors.lastname: Pikuleva
authors.forename: S A
authors.initials: SA
authors.lastname: Usanov
authors.forename: V L
authors.initials: VL
authors.lastname: Chashchin
mesh_terms: D000302:Adrenal Cortex / Q000201:enzymology*; D000325:Adrenodoxin / Q000378:metabolism; D000818:Animals; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004047:Diethyl Pyrocarbonate / Q000494:pharmacology*; D005561:Formates / Q000494:pharmacology*; D006639:Histidine / Q000032:analysis; D066298:In Vitro Techniques; D007700:Kinetics; D008928:Mitochondria / Q000201:enzymology*; D010084:Oxidation-Reduction
chemical_list: D005561:Formates; D000325:Adrenodoxin; D006639:Histidine; D002786:Cholesterol Side-Chain Cleavage Enzyme; D004047:Diethyl Pyrocarbonate
vernacular_title: Khimicheskaia modifikatsiia kholesteringidroksiliruiushchego tsitokhroma P-450 mitokhondriĭ kory nadpochechnikov diétilpirokarbonatom.