Synthetic fragments and analogues of elastin. I. The synthesis.
The synthesis of some repetitive sequences of elastin and their simplified analogues, all comprising the structural unit Gly-X-Gly (X = Val, Leu, Ala), is described. In particular, the following peptides and polypeptides were synthesized and characterized: Boc-Gly-Val-Gly-Gly-Leu-OMe, Boc-Gly-Leu-Gly-Gly-Val-OMe, Boc-(Gly-Val-Gly-Gly-Leu)2-OMe, Boc-(Gly-Val-Gly-Gly-Leu)3-OMe, Boc-Gly-Val-Gly-Gly-OEt, Boc-Leu-Gly-Gly-Leu-OMe, Boc-Val-Gly-Gly-Val-OMe, poly(Ala-Gly-Gly), poly(Val-Gly-Gly), and poly(Leu-Gly-Gly). In every case, the synthesis was accomplished by classical procedures in solution, by using the p-nitrophenyl ester method for the polycondensation step, and the mixed anhydride or the azide methods for the coupling steps.
Guantieri
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V
Department of Inorganic, Metallorganic and Analytical Chemistry, University of Padova, Italy.
Grando
S
S
Pandolfo
L
L
Tamburro
A M
AM
D000595:Amino Acid Sequence; D004549:Elastin / Q000031:analogs & derivatives*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis*
D010446:Peptide Fragments; D004549:Elastin