Reversibility of tributyltin-chloride-induced protein synthesis inhibition after ATP recovery in HEL-30 cells.
The effect of tributyltin chloride (TBT) on ATP levels and protein synthesis was investigated in a murine epidermal cell line (HEL-30). Five minutes after exposure to 10(-5) M TBT, cellular levels of ATP decreased by 57% and protein synthesis was significantly inhibited. The effect of ATP was stable up to 2 h incubation, whereas inhibition of protein synthesis increased with time of treatment. Partial (1 h) or complete (2 h) recovery of the two cell functions was observed when dithiothreitol (DTT) was added to the medium, 5 min after the damage and in the absence of TBT. The sequence of the observed effects suggests that TBT inhibition of protein synthesis is due to impairment of ATP production.
Marinovich
M
M
Institute of Pharmacological Sciences, University of Milan, Italy.
Viviani
B
B
Galli
C L
CL
D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000818:Animals; D002460:Cell Line / Q000187:drug effects; D004229:Dithiothreitol / Q000494:pharmacology; D007930:Leucine / Q000378:metabolism; D051379:Mice; D014176:Protein Biosynthesis; D014220:Trialkyltin Compounds / Q000037:antagonists & inhibitors / Q000633:toxicity*
D014220:Trialkyltin Compounds; C011559:tributyltin; D000255:Adenosine Triphosphate; D007930:Leucine; D004229:Dithiothreitol