Influence of temperature on degradation kinetics of ceftriaxone in diluted and undiluted human serum.
The stability of ceftriaxone in undiluted human serum and in human serum diluted 1/20 in 0.1 M phosphate buffer (pH 6.0) was measured at -70, -40, -20, 4, and 37 degrees C. Ceftriaxone in diluted human serum contained at least 90% of the initial activity after 3 months of storage at -20, -40, and -70 degrees C; unbuffered human serum contained 82.41, 84.92, and 88.96% of the initial activity, respectively. Ceftriaxone in unbuffered human serum and in diluted human serum showed 80.33 and 86.25% of the initial activity, respectively, after 55 days at 4 degrees C. After 120 h at 37 degrees C, this antibiotic in unbuffered human serum contained 33.08% of the initial activity, whereas samples of diluted human serum contained more than 60%. Consequently, the stability of antibiotic in human serum diluted 1/20 in 0.1 M phosphate buffer (pH 6.0) is increased.
Esteban
M J
MJ
Unit of Experimental Bacteriology, Investigation Center, Hospital, La Fe, Valencia, Spain.
Cantón
E
E
Rius
F
F
D002021:Buffers; D002443:Ceftriaxone / Q000097:blood*; D004355:Drug Stability; D006801:Humans; D013048:Specimen Handling; D013696:Temperature
D002021:Buffers; D002443:Ceftriaxone