Identification of novel gangliosides containing lactosaminyl-GM1 structure from rat spleen.
Two novel monosialogangliosides were isolated from rat spleen. The structures of the gangliosides (shown below, where NeuNGc is N-glycolylneuraminic acid) were characterized by compositional analysis, methylation analysis, hydrolyses with exoglycosidases, direct probe fast atom bombardment mass spectrometry, and proton nuclear magnetic resonance spectrometry. A novel structure common to both gangliosides was N-acetyllactosaminyl-GM1 LacN-GM1; where GM1 is II3NeuAc-GgOse4Cer), and this is the first paper to report the occurrence of a new group of gangliosides. [formula: see text] Furthermore, in a monosialoganglioside fraction of rat spleen, the occurrence of a ganglioside having two lactosamine units (Gal alpha 1-3(Gal beta 1-4GlcNAc beta 1-3)2-GM1(NeuAc) (alpha Gal-(LacN)2-GM1] was suggested. These gangliosides have a unique structure, which includes the ganglio series ganglioside core and the extended modification characteristic of the lacto series.
Nohara
K
K
National Institute for Environmental Studies, Tsukuba, Ibaraki, Japan.
Suzuki
M
M
Inagaki
F
F
Ito
H
H
Kaya
K
K
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D005677:G(M1) Ganglioside / Q000031:analogs & derivatives* / Q000032:analysis; D005732:Gangliosides / Q000302:isolation & purification*; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000032:analysis*
D005227:Fatty Acids; D005732:Gangliosides; C065421:lactosaminyl-G(M1); D005677:G(M1) Ganglioside