Investigations on the fluorescence concentration quenching of flavomononucleotide in glycerine-water solutions.
We measured concentration changes of the fluorescence quantum yield eta/eta 0 of flavomononucleotide (FMN) in glycerine-water solutions of various viscosities. The results obtained show that FMN dimers are traps for the exciting light energy as well as the energy transferred non-radiatively. Very good agreement between the experimental and theoretical eta/eta 0 results was obtained. It has been shown that in the investigated solutions non-radiative energy transfer from monomers to dimers takes place, preceded by the migration of energy. It has been stated that no monomer quenching occurs in these solutions. The contribution of the individual fluorescence concentration quenching mechanisms has been determined.
Grajek
H
H
Institute of Physics and Food Chemistry, Academy of Agriculture and Technology, Olsztyn, Poland.
Zurkowska
G
G
Bojarski
C
C
Drabent
R
R
D005486:Flavin Mononucleotide; D005990:Glycerol; D008433:Mathematics; D012996:Solutions; D013050:Spectrometry, Fluorescence; D014867:Water
D012996:Solutions; D014867:Water; D005486:Flavin Mononucleotide; D005990:Glycerol