title: Isolation and characterization of DNA sequences from flow-sorted human chromosome 22 libraries.
abstract: Two flow-sorted chromosome 22 libraries were used to isolate DNA sequences specific for chromosome 22. 45-phage DNAs were probed against human genomic DNA. 12 of them showed unique or low-copy character. Using digested DNA from rodent-human hybrid cell lines, 3 of the 12 recombinants were assigned unique to chromosome 22 and regionally mapped. 1 clone mapped to 22pter-q11, 1 clone to 22q12-qter and 1 clone, for which in situ hybridization was performed, to 22q13.1. 2 low-copy probes, 1 of them displaying polymorphisms in MspI and TaqI digests of individual DNAs, must have similar sequences on 22 and additional chromosomes. Furthermore, a highly repetitive DNA representing a compound locus of some hundred kilobases on chromosome 22 was isolated. These 6 probes may provide useful tools for studying the structure and function of this small chromosome involved in a relatively high number of inherited and acquired diseases.
authors.affiliation: Department of Human Genetics, Saar University, Homburg, FRG.
authors.forename: R
authors.initials: R
authors.lastname: Metzdorf
authors.forename: R
authors.initials: R
authors.lastname: Herzog
authors.forename: E
authors.initials: E
authors.lastname: Göttert
authors.forename: N
authors.initials: N
authors.lastname: Blin
mesh_terms: D002892:Chromosomes, Human, Pair 22; D015342:DNA Probes / Q000302:isolation & purification*; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D005434:Flow Cytometry; D015723:Gene Library; D006801:Humans; D010375:Pedigree; D011110:Polymorphism, Genetic
chemical_list: D015342:DNA Probes; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII