31P NMR of mammalian cells encapsulated in alginate gels utilizing a new phosphate-free perfusion medium.
A simple technique permitting long term 31P magnetic resonance spectroscopy of cultured mammalian cells is described. Cells are encapsulated in calcium alginate gels and perfused with a new phosphate-fre medium designed for maintenance of high cell viability. Intracellular pH and beta-NTP/Pi ratios, studied in three cell types, remained stable over a 16-20 h NMR monitoring period. The techniques described may be useful for monitoring cell metabolism with a variety of cell types.
Narayan
K S
KS
Huntington Medical Research Institutes, Pasadena, CA 91101.
Moress
E A
EA
Chatham
J C
JC
Barker
P B
PB
D000464:Alginates; D002118:Calcium; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media; D005782:Gels; D006801:Humans; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010710:Phosphates; D010758:Phosphorus
D000464:Alginates; D003470:Culture Media; D005782:Gels; D010710:Phosphates; D010758:Phosphorus; D002118:Calcium