title: cDNA cloning and sequence analysis of the 3'-terminal region of zucchini yellow mosaic virus RNA.
abstract: The 3' half of the RNA of the cucurbit potyvirus zucchini yellow mosaic virus (ZYMV) was genetically cloned and the cDNA sequence of a portion of the putative RNA polymerase gene, the complete coat protein gene and the 3' untranslated region was determined. The coat protein and putative polymerase genes are both part of a continuous open reading frame as is the case for other potyviruses whose genomes are expressed as polyproteins. The Q/S protease cleavage site for the N terminus of the coat protein was deduced by alignment of the coat protein and polymerase genes with other potyviral sequences. The resulting protein has 279 amino acids and a calculated Mr of 31,214. The predicted amino acid sequence indicates a ZYMV-unique N-terminal region and potyvirus-characteristic central and C-terminal regions. These data also verify that ZYMV is distinct from the cucurbit potyvirus watermelon mosaic virus 2.
authors.affiliation: Department of Horticulture, Michigan State University, East Lansing 48824.
authors.forename: R
authors.initials: R
authors.lastname: Grumet
authors.forename: G W
authors.initials: GW
authors.lastname: Fang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000235:genetics; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral; D012321:DNA-Directed RNA Polymerases