Detection of benzoylecgonine (cocaine metabolite) in urine: a cost-effective low risk immunoassay procedure.
Using Syva EMIT reagents and a Cobas-Bio centrifugal analyser we have developed a cost-effective assay for the detection in urine of benzoylecgonine, one of the main metabolites of cocaine. With this method up to 2470 samples can be assayed with a single 100-test EMIT Kit while maintaining acceptable precision. A mean CV of 7.0% was obtained for the concentration range 240-620 micrograms/l. Comparison of the method with the Abbott TDx system showed good correlation. The Cobas-Bio method is suitable for high-risk urines since heat treatment may be performed prior to analysis.
Moore
F M
FM
Department of Clinical Chemistry, Royal Infirmary, Edinburgh, Scotland, UK.
Simpson
D
D
D003042:Cocaine / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D007118:Immunoassay / Q000191:economics / Q000379:methods* / Q000706:statistics & numerical data; D019966:Substance-Related Disorders / Q000652:urine
C005618:benzoylecgonine; D003042:Cocaine