Increase of deleted mitochondrial DNA in the striatum in Parkinson's disease and senescence.
A mutant mitochondrial DNA (mtDNA) with a 4,977-bp deletion was detected in the parkinsonian brain by using the polymerase chain reaction. Although the deleted mtDNA was detectable even in the brain of aged controls, the proportion of deleted mtDNA to normal mtDNA in the striatum was higher in the parkinsonian patients than in the controls. In both the parkinsonian patients and the aged controls, the proportion was higher in the striatum than in the cerebral cortex. These results indicate that age-related accumulation of deleted mtDNA is accelerated in the parkinsonian striatum and suggest that the deletion contributes to pathophysiological processes underlying Parkinson's disease.
Ikebe
S
S
Department of Biomedical Chemistry, Faculty of Medicine, University of Nagoya, Japan.
Tanaka
M
M
Ohno
K
K
Sato
W
W
Hattori
K
K
Kondo
T
T
Mizuno
Y
Y
Ozawa
T
T
D000328:Adult; D000368:Aged; D000375:Aging / Q000235:genetics*; D001483:Base Sequence; D003342:Corpus Striatum / Q000032:analysis*; D004272:DNA, Mitochondrial / Q000032:analysis / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D010300:Parkinson Disease / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction
D004272:DNA, Mitochondrial