title: Rapid detection of chromosome 16 inversion in acute nonlymphocytic leukemia, subtype M4: regional localization of the breakpoint in 16p.
abstract: The pericentric inversion of chromosome 16 characteristic for acute nonlymphocytic leukemia, subtype M4, was detected in five patients by means of nonradioactive in situ hybridization of complete cosmids. First, five cosmids situated along the short arm of chromosome 16 were used to map the breakpoint of the inversion distal to the rare folate-sensitive fragile site FRA16A. Then, the use of two cosmids on either side of the breakpoint, combined with a probe specific for the centromeric region of chromosome 16, readily detected the inversion, even in poor metaphase spreads.
authors.affiliation: Department of Human Genetics, State University of Leiden, The Netherlands.
authors.forename: J G
authors.initials: JG
authors.lastname: Dauwerse
authors.forename: T
authors.initials: T
authors.lastname: Kievits
authors.forename: G C
authors.initials: GC
authors.lastname: Beverstock
authors.forename: D
authors.initials: D
authors.lastname: van der Keur
authors.forename: E
authors.initials: E
authors.lastname: Smit
authors.forename: H W
authors.initials: HW
authors.lastname: Wessels
authors.forename: A
authors.initials: A
authors.lastname: Hagemeijer
authors.forename: P L
authors.initials: PL
authors.lastname: Pearson
authors.forename: G J
authors.initials: GJ
authors.lastname: van Ommen
authors.forename: M H
authors.initials: MH
authors.lastname: Breuning
mesh_terms: D007446:Chromosome Inversion; D002874:Chromosome Mapping; D002885:Chromosomes, Human, Pair 16; D003360:Cosmids; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D009693:Nucleic Acid Hybridization