Translational or post-translational processes affect differentially the accumulation of isocitrate lyase and malate synthase proteins and enzyme activities in embryos and seedlings of Brassica napus.
We have analyzed the accumulation of the glyoxylate cycle enzymes isocitrate lyase and malate synthase in embryos and seedlings of Brassica napus L. The two enzyme activities and proteins begin to accumulate during late embryogeny, reach maximal levels in seedlings, and are not detected in young leaves of mature plants. We showed previously that mRNAs encoding the two enzymes exhibit similar qualitative patterns of accumulation during development and that the two mRNAs accumulate to different levels in both embryos and seedlings (L. Comai et al., 1989, Plant Cell 1, 293-300). In this report, we show that the relative accumulation of the proteins and activities do not correspond to these mRNA levels. In embryos and seedlings, the specific activities of isocitrate lyase and malate synthase are approximately constant. By contrast, the ratio of malate synthase protein to mRNA is 14-fold higher than that of isocitrate lyase. Differences in the translational efficiencies of the two mRNAs in vitro do not appear to account for the discrepancy between mRNA and protein levels. Our results suggest that translational and/or post-translational processes affect differentially the accumulation of the proteins.
Ettinger
W F
WF
Department of Botany, University of California, Davis 95616.
Harada
J J
JJ
D001937:Brassica / Q000201:enzymology* / Q000254:growth & development; D007522:Isocitrate Lyase / Q000378:metabolism*; D008292:Malate Synthase / Q000378:metabolism*; D007652:Oxo-Acid-Lyases / Q000378:metabolism*; D011499:Protein Processing, Post-Translational / Q000502:physiology*
D008292:Malate Synthase; D007652:Oxo-Acid-Lyases; D007522:Isocitrate Lyase