In situ (6-4)photoproduct determination by laser cytometry and autoradiography.
The UV-induced (6-4)photoproducts and their repair in individual human cells were quantitatively determined by argon-laser imaging microspectrofluorometry or autoradiography with a well-characterized monoclonal antibody against (6-4)photoproducts. (6-4)Photoproduct induction curves were linear as a function of UV dose, using both methods. The formation of (6-4)photoproducts was detected in the cells irradiated with as low as 10 and 25 J/m2 of UV by autoradiography and laser cytometry, respectively. Normal cells repaired more than 80% of the initial damage within 4 h post irradiation. In contrast, almost no repair was observed in xeroderma pigmentosum cells (complementation group A) within 8 h.
Mori
T
T
Division of Radiation Biology, Faculty of Pharmaceutical Sciences, Kanazawa University, Japan.
Matsunaga
T
T
Chang
C C
CC
Trosko
J E
JE
Nikaido
O
O
D000911:Antibodies, Monoclonal; D001345:Autoradiography / Q000379:methods; D002478:Cells, Cultured; D004249:DNA Damage; D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts / Q000166:cytology / Q000528:radiation effects; D006801:Humans; D007700:Kinetics; D007834:Lasers; D008856:Microscopy, Fluorescence / Q000379:methods; D013997:Time Factors; D014466:Ultraviolet Rays; D014983:Xeroderma Pigmentosum
D000911:Antibodies, Monoclonal