Increased expression of alkaline phosphatase activity in stimulated B lymphocytes by muramyl dipeptide.
We have recently shown that the synthetic immunomodulator muramyl dipeptide (MDP) acts on murine B lymphocytes. It synergizes with interleukin 2 and interleukin 4 to stimulate, respectively, the differentiation and the proliferation of B cells. In the present study, MDP was shown to increase the proliferation of B cells stimulated by lipopolysaccharide (LPS). Moreover, the expression of alkaline phosphatase activity induced by LPS was markedly enhanced by MDP. These effects were time- and dose-dependent. The present report suggests that the biochemical mechanism by which MDP exerts its effects may involve protein phosphorylation-dephosphorylation pathways.
Souvannavong
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CNRS URA 1116, Institut de Biochimie, Université Paris-Sud, Orsay, France.
Adam
A
A
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000494:pharmacology*; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004357:Drug Synergism; D005260:Female; D007376:Interleukin-2 / Q000494:pharmacology; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D011994:Recombinant Proteins / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D000119:Acetylmuramyl-Alanyl-Isoglutamine; D000469:Alkaline Phosphatase