Effect of ferric nitrilotriacetate on predominantly cortical glial cell cultures.
Cultured glial cells were exposed to ferric nitrilotriacetate (Fe-NTA) at varying concentrations. Studies of the exposed glial cells were performed at days 29 and 36 post-conceptional age (culture days 8 and 15). In addition to morphologic studies, biochemical assays including [3H]-flunitrazepam (FLU) specific binding, Ro5-4864-displaceable 3H-FLU binding, and protein determinations were performed. At day 29 post-conceptional age, significant decreases in 3H-FLU specific binding, Ro5-4864-displaceable 3H-FLU binding, and protein determinations were discernible only in the presence of 100 microM Fe-NTA. At day 36 post-conceptional age 3H-FLU specific binding was significantly decreased at 20, 60, and 100 microM Fe-NTA concentrations, while Ro5-4864-displaceable 3H-FLU binding and protein determinations were significantly reduced at 60 and 100 microM Fe-NTA concentrations. The effects of Fe-NTA exposure appear to be both concentration and duration-of-exposure related. When compared to previously reported neuronal cell culture studies utilizing 3H-FLU specific binding, Ro5-4864-displaceable 3H-FLU binding, and protein determinations, glial cells appear to be significantly more resistant to chelated iron exposure.
Swaiman
K F
KF
Division of Pediatric Neurology, University of Minnesota Medical School, Minneapolis.
Machen
V L
VL
D000085:Acetates / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D001570:Benzodiazepinones / Q000378:metabolism; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D005290:Ferric Compounds / Q000494:pharmacology*; D005445:Flunitrazepam / Q000378:metabolism; D051379:Mice; D009457:Neuroglia / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism; D013997:Time Factors
D000085:Acetates; D001570:Benzodiazepinones; D005290:Ferric Compounds; D011506:Proteins; C028513:4'-chlorodiazepam; D005445:Flunitrazepam; D009571:Nitrilotriacetic Acid; C020326:ferric nitrilotriacetate