title: T cell depletion from human bone marrow using magnetic beads.
abstract: We have studied a recently described method to purge human marrow of T cells using magnetic beads conjugated to various anti-T cell monoclonal antibodies. Using anti-CD2 and anti-T cell receptor/CD3 monoclonal antibodies, we show that up to 2.76 log T cell purge can be achieved while recovery of hemopoietic activity is 82%. As a control, our clinically established T cell depletion protocol, namely soybean agglutination combined with E-rosetting, provided 3.39 log T cell purge and 59% recovery of hemopoietic activity. The impact of these results on T cell depletion strategies in HLA-nonidentical bone marrow transplantation is discussed.
authors.affiliation: Department of Pediatrics II, University of Ulm, FRG.
authors.forename: C
authors.initials: C
authors.lastname: Knobloch
authors.forename: U
authors.initials: U
authors.lastname: Spadinger
authors.forename: E
authors.initials: E
authors.lastname: Rueber
authors.forename: W
authors.initials: W
authors.lastname: Friedrich
mesh_terms: D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation / Q000276:immunology / Q000379:methods*; D005069:Evaluation Studies as Topic; D006086:Graft vs Host Disease / Q000517:prevention & control; D006680:HLA Antigens; D006239:Haplotypes; D006801:Humans; D037102:Lectins; D008212:Lymphocyte Depletion; D008280:Magnetics; D037121:Plant Lectins; D012397:Rosette Formation; D030262:Soybean Proteins; D013601:T-Lymphocytes / Q000276:immunology; D010835:Phytohemagglutinins
chemical_list: D006680:HLA Antigens; D037102:Lectins; D037121:Plant Lectins; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins