Detection of Chlamydia trachomatis in formalin-fixed endometrial tissue.
Formalin-fixed tissue biopsies from 29 women with the histological diagnosis of severe endometritis were examined for chlamydial antigen by fluorescein-conjugated monoclonal chlamydial antibodies (SYVA) technique; 15 (52%) were positive. In nine patients also samples for isolation of Chlamydia trachomatis by cell-culture technique were obtained peroperatively; seven were culture-positive. In all these patients fluorescent chlamydial antigen was detected, whereas the two culture-negative patients had no such antigen. The technique presented makes it possible to examine series of formalin-fixed endometrical biopsies to evaluate the significance of C. trachomatis being etiologic agent in endometritis in females.
Ersbak
V
V
Department of Gynecology, University Hospital, Arhus, Denmark.
Søgaard
H
H
Møller
B R
BR
D000293:Adolescent; D000328:Adult; D000942:Antigens, Bacterial / Q000032:analysis; D001706:Biopsy; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000276:immunology; D002692:Chlamydia trachomatis / Q000276:immunology; D004717:Endometrium / Q000382:microbiology / Q000473:pathology; D005260:Female; D005557:Formaldehyde; D006801:Humans; D008875:Middle Aged; D014021:Tissue Preservation / Q000379:methods
D000942:Antigens, Bacterial; D005557:Formaldehyde