title: Pyruvate uptake induced by a pH jump in mesophyll chloroplasts of maize and sorghum, NADP-malic enzyme type C4 species.
abstract: A sudden pH decrease (pH jump) of the medium enhanced pyruvate uptake in the dark in mesophyll chloroplasts (MCp) of Zea mays and Sorghum bicolor, NADP-malic enzyme type C4 plants, while it was reported that a Na+ jump enhanced pyruvate uptake in MCp of P. miliaceum, a NAD-malic enzyme type [(1987) FEBS Lett. 219, 347]. The enhancement effect of the pH jump decayed completely in 5 min and the decay was accelerated by proton gradient-collapsing reagents. The results suggest that active pyruvate uptake into MCp of NADP-malic enzyme type C4 species is primarily driven by the proton gradient across the envelope.
authors.affiliation: Department of Biochemistry, Faculty of Science, Saitama University, Urawa, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Ohnishi
authors.forename: R
authors.initials: R
authors.lastname: Kanai
mesh_terms: D001693:Biological Transport, Active; D002736:Chloroplasts / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008027:Light; D008291:Malate Dehydrogenase / Q000378:metabolism; D008564:Membrane Potentials; D009249:NADP / Q000378:metabolism; D009944:Organoplatinum Compounds; D011773:Pyruvates / Q000378:metabolism*; D013997:Time Factors; D003313:Zea mays
chemical_list: D009944:Organoplatinum Compounds; D011773:Pyruvates; D009249:NADP; C052443:di-(2-amino-5-nitrothiazole)dichloroplatinum(II); D008291:Malate Dehydrogenase