title: Complete cDNA sequence of a South American isolate of potato virus X.
abstract: The complete cDNA sequence corresponding to the genomic RNA of a South American strain of potato virus X (PVXc) is reported. The sequence (6432 nucleotides) contains five open reading frames coding for polypeptides with molecular weights of 165.3, 24.3, 12.3, 7.6 and 25.0 and displays an overall homology of 77.4% with those previously reported for two European isolates. Comparison of amino acid sequences shows an average homology of 87%. Two major domains of variability, located between amino acids 476-615 of ORF 1 and 64-100 of ORF 5, are identified. Sequence similarities between RNA stretches lying upstream of ORFs 2, 4 and 5, and at the 3'-non coding regions of PVX and other plus-strand RNA viruses are described.
authors.affiliation: Instituto de Investigaciones en Ingenieria Genética y Biologia Molecular, CONICET, Buenos Aires, Argentina.
authors.forename: B E
authors.initials: BE
authors.lastname: Orman
authors.forename: R M
authors.initials: RM
authors.lastname: Celnik
authors.forename: A M
authors.initials: AM
authors.lastname: Mandel
authors.forename: H N
authors.initials: HN
authors.lastname: Torres
authors.forename: A N
authors.initials: AN
authors.lastname: Mentaberry
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral; D005060:Europe; D005814:Genes, Viral; D008969:Molecular Sequence Data; D010942:Plant Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D011198:Solanum tuberosum / Q000382:microbiology; D013020:South America
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral