title: Ontogeny of growth hormone (GH) binding in the domestic turkey: evidence of sexual dimorphism and developmental changes in relationship to plasma GH.
abstract: The post-hatch ontogeny of hepatic GH binding and its relationship to GH plasma profile characteristics in male and female turkeys of slow- (RBC-2) and fast-growing (F; selected from RBC-2) genetic lines were determined. Specific binding of 125I-labelled recombinant chicken GH to crude hepatic membrane preparations (100,000 g pellet) was determined at 2, 4, 8, 14 and 24 weeks of age for both total (occupied plus free; 4 mol MgCl2/l pretreatment) and free (without MgCl2 pretreatment) binding sites. Characteristics of the plasma GH profile were measured at each age by serial blood sampling through indwelling jugular vein catheters. When specific binding to either free or total sites was expressed on a whole organ basis (i.e. hepatic GH-binding capacity/bird), binding increased dramatically (P less than 0.0001) with increasing age over both lines and sexes. Total binding capacity (free plus occupied sites) per bird was greater for females than for males at 24 weeks of age (P less than 0.04), as birds reached sexual maturity, but did not differ between fast- and slow-growing lines at any age. Available binding capacity (free sites) per bird was greater for the faster growing F than RBC-2 line at the older ages when body size was most divergent (14 and 24 weeks of age; P less than 0.01, P less than 0.06 respectively), but did not differ between sexes. Correlation analysis at individual ages revealed a progressive change in the nature of the relationship between hepatic GH binding, plasma GH and somatic growth.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Poultry Science, Pennsylvania State University, University Park 16802.
authors.forename: R
authors.initials: R
authors.lastname: Vasilatos-Younken
authors.forename: K S
authors.initials: KS
authors.lastname: Gray
authors.forename: W L
authors.initials: WL
authors.lastname: Bacon
authors.forename: K E
authors.initials: KE
authors.lastname: Nestor
authors.forename: D W
authors.initials: DW
authors.lastname: Long
authors.forename: J L
authors.initials: JL
authors.lastname: Rosenberger
mesh_terms: D000375:Aging / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D005260:Female; D013006:Growth Hormone / Q000097:blood / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism; D008099:Liver / Q000033:anatomy & histology; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D009929:Organ Size; D012737:Sex Factors; D012741:Sexual Maturation; D014422:Turkeys / Q000235:genetics*; D015430:Weight Gain
chemical_list: D013006:Growth Hormone