An easy method for impaling cells using the micromanipulation technique.
In the method described, the head of a fixed-stage microscope and the attached micromanipulator are simultaneously displaced during focussing movements of the microscope. This design allows axial displacements of the microscope towards the cell to be injected. This arrangement makes the filling of neurons in brain slices by intracellular iontophoresis injection of Lucifer yellow considerably easier.
Perez-Samartin
A L
AL
Department of Neurosciences, Universidad del País Vasco, Leioa, Spain.
Martinez-Millan
L
L
Doñate-Oliver
F
F
D000818:Animals; D001921:Brain / Q000166:cytology*; D002415:Cats; D005456:Fluorescent Dyes; D007478:Iontophoresis / Q000295:instrumentation* / Q000379:methods; D007546:Isoquinolines; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods
D005456:Fluorescent Dyes; D007546:Isoquinolines; C017475:lucifer yellow