Paracrine growth stimulation of androgen-responsive Shionogi Carcinoma 115 by its autonomous subline (Chiba Subline 2).
Shionogi Carcinoma 115 cells (SC 115 cells) and Chiba Subline 2 cells (CS 2 cells) are clones of an androgen-responsive mouse tumor cell line and its autonomous subline, respectively. Since it was reported that the growth of cells from SC 115 was regulated by an androgen-induced fibroblast growth factor (FGF)-like peptide (1), the present study was aimed at examining whether a similar growth factor was secreted by CS 2 cells in the absence of testosterone. Although SC 115 cells did not grow in the serum-free medium without androgens, SC 115 cells could proliferate in mixed culture with CS 2 cells, suggesting stimulation of SC 115 cells by CS 2 cells. It was shown that CS 2 cells secreted a growth factor without the influence of testosterone, and this factor promoted the growth of SC 115 and CS 2 cells, as well as that of BALB/3T3 cells. The factor was partially purified from serum-free conditioned medium obtained from cultures of CS 2 cells. It showed an affinity for heparin, stability to heat and acid treatments, a decrease in activity when anti-basic FGF antibody was added to cultures, and an estimated molecular weight of approximately 50,000. Therefore, the factor seemed to have the nature of an FGF-like peptide. It was concluded that, in the absence of testosterone, CS 2 cells produced an FGF-like growth factor which controlled the growth of both CS 2 cells and parent SC 115 cells, in autocrine and paracrine manners, respectively.
Furuya
Y
Y
Department of Urology, School of Medicine, Chiba University, Japan.
Sato
N
N
Akakura
K
K
Ichikawa
T
T
Suzuki
N
N
Sato
R
R
Shimazaki
J
J
D000818:Animals; D002450:Cell Communication; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002846:Chromatography, Affinity; D004261:DNA Replication; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D005346:Fibroblast Growth Factors / Q000276:immunology / Q000494:pharmacology*; D006133:Growth Substances / Q000302:isolation & purification / Q000502:physiology*; D051379:Mice; D008970:Molecular Weight; D013739:Testosterone / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000166:cytology* / Q000187:drug effects
D004273:DNA, Neoplasm; D006133:Growth Substances; D013739:Testosterone; D005346:Fibroblast Growth Factors; D013936:Thymidine