title: Neuropeptides from the sinus gland of the lobster Homarus americanus: Characterization of hyperglycemic peptides.
abstract: In order to characterize hyperglycemic peptides from the sinus gland of the lobster, Homarus americanus, a bioassay was developed with juvenile H. gammarus. This assay was used for determining the hyperglycemic activity of peptides perified by reversed-phase high-performance liquid-chromatography, from acidic extracts of sinus gland. The major peptides are eluted in three sets of two peptides. Among them, two pairs show hyperglycemic activity when assayed on lobster; when assayed on crayfish, three peptides are active. The less hydrophobic pair consists of basic peptides (pI: 8.7), with a MW of 8633 Da., determined by fast-atom bombardment mass spectrometry. The most hydrophobic pair consists of acid peptides (pI: 5.0), with a MW of 8577 Da. Amino acid composition of the hyperglycemic peptides shows strong homologies within each pair.
authors.affiliation: Laboratoire de Neuroendocrinologie des Crustacés, URA 555 CNRS, Université Pierre et Marie Curie, Paris, France.
authors.forename: D
authors.initials: D
authors.lastname: Soyez
authors.forename: P Y
authors.initials: PY
authors.lastname: Noel
authors.forename: J E
authors.initials: JE
authors.lastname: Van Deijnen
authors.forename: M
authors.initials: M
authors.lastname: Martin
authors.forename: A
authors.initials: A
authors.lastname: Morel
authors.forename: G G
authors.initials: GG
authors.lastname: Payen
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D060829:Arthropod Proteins; D003400:Astacoidea / Q000187:drug effects / Q000378:metabolism; D001681:Biological Assay; D001786:Blood Glucose / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005123:Eye; D007447:Invertebrate Hormones; D007526:Isoelectric Point; D013058:Mass Spectrometry; D008970:Molecular Weight; D008121:Nephropidae / Q000187:drug effects / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D009479:Neuropeptides / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000596:Amino Acids; D060829:Arthropod Proteins; D001786:Blood Glucose; D007447:Invertebrate Hormones; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; C015947:hyperglycemic hormone, crustacean