Interactions of micromolar concentrations of fluoride with Streptococcus rattus FA-1.
Inhibition of the metabolic activities of bacteria by trace amounts of fluoride is manifested phenomenologically as changes in the pH gradient and/or the electrical potential between the cellular interior and the surrounding medium. These data were obtained from the intracellular/extracellular distribution of radioactivity labelled fluoride (18F), 5,5-dimethyloxazolidine-2,4-dione (14C), and tetraphenylphosphonium chloride (14C). When taken up from acidic media, trace concentrations of fluoride (1-100 microM) reduce the intracellular/extracellular pH gradient and affect the electrical potential across the cell membrane. The chromatographic fractionation of fluoride-charged bacterial homogenates showed that fluoride is attached to many proteins of the cytoplasm, the cell membrane, and to nonproteinaceous components of the cell wall. Lysozyme treatment synergistically affects the vulnerability of the bacteria to micromolar concentrations of fluoride.
Psarros
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Fachbereich Physikalische Chemie, Kernchemie, Philipps-Universität, Marburg, FRG.
Feige
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Duschner
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D001426:Bacterial Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004114:Dimethadione / Q000378:metabolism; D005459:Fluorides / Q000494:pharmacology*; D008564:Membrane Potentials; D010751:Phosphopyruvate Hydratase / Q000037:antagonists & inhibitors; D012044:Regression Analysis; D013291:Streptococcus / Q000187:drug effects* / Q000201:enzymology
D001426:Bacterial Proteins; D004114:Dimethadione; D010751:Phosphopyruvate Hydratase; D005459:Fluorides