title: The stereospecificities of seven dehydrogenases from Acholeplasma laidlawii. The simplest historical model that explains dehydrogenase stereospecificity.
abstract: Stereospecificities are reported for seven dehydrogenases from Acholeplasma laidlawii, an organism from an evolutionarily distinct branch of life which has not previously been studied from a stereochemical point of view. Three of the activities examined (alcohol dehydrogenase, lactate dehydrogenase, and alanine dehydrogenase) catalyze the transfer of the pro-R (A) hydrogen from NADH. Four other activities (3-hydroxy-3-methylglutaryl-CoA reductase, glyceraldehyde-3-phosphate dehydrogenase, glucose-6-phosphate dehydrogenase, and NADH oxidase) catalyze the transfer of the pro-S (B) hydrogen from NAD(P)H. The stereospecificity of hydroxymethylglutaryl-CoA reductase is notable because it is the opposite of that of hydroxymethylglutaryl-CoA reductases from yeast and rat. These data are used to derive the simplest historical model capable of explaining available experimental facts.
authors.affiliation: Laboratory for Organic Chemistry, Swiss Federal Institute of Technology, Zurich.
authors.forename: A
authors.initials: A
authors.lastname: Glasfeld
authors.forename: G F
authors.initials: GF
authors.lastname: Leanz
authors.forename: S A
authors.initials: SA
authors.lastname: Benner
mesh_terms: D000128:Acholeplasma laidlawii / Q000201:enzymology*; D050788:Alanine Dehydrogenase; D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000594:Amino Acid Oxidoreductases / Q000378:metabolism; D005075:Biological Evolution; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008954:Models, Biological; D009097:Multienzyme Complexes / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D009097:Multienzyme Complexes; D010088:Oxidoreductases; D006903:Hydroxymethylglutaryl CoA Reductases; D000426:Alcohol Dehydrogenase; D007770:L-Lactate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D000594:Amino Acid Oxidoreductases; D050788:Alanine Dehydrogenase; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases