A sandwich ELISA with monoclonal anti-IgG allotype antibodies permitting the deduction of the G1m genotype.
With murine monoclonal antibodies specific for the human IgG1 allotypes G1m(z), G1m(a), G1m(f) and the IgG1 subclass, sandwich enzyme-linked immunosorbent assays (ELISAs) were developed to determine whether different allotypic determinants are located on the same molecule. With the conventional haemagglutination-inhibition test, it is only possible to determine the phenotype. With this ELISA system, it is now possible to deduce the G1m genotype.
van Leeuwen
A M
AM
Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, University of Amsterdam.
de Lange
G G
GG
D000483:Alleles; D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007129:Immunoglobulin Gm Allotypes / Q000235:genetics*; D007214:Indonesia; D010375:Pedigree; D010641:Phenotype; D013785:Thailand
D000911:Antibodies, Monoclonal; D007129:Immunoglobulin Gm Allotypes