title: Characterization of the cellular antigens of Paracoccidioides brasiliensis yeast form.
abstract: Antigenic components of the yeast extract of Paracoccidioides brasiliensis Linder 2511 cultured for 3, 8, 20, 30, and 60 days were examined by the Western blot (immunoblot) technique. The 3-day extract was chosen for characterization of the antigenic components because its stability did not vary with time and it contained all antigens identified by patient sera. Antibodies to cross-reacting antigens of P. brasiliensis extracts were detected in sera from patients with histoplasmosis, candidiasis, and aspergillosis. The 58-, 57-, 21-, and 16-kilodalton (kDa) antigens were specific for P. brasiliensis, while the 48- and 45-kDa antigens were specific for paracoccidioidomycosis. The Western blot technique is a useful tool for the diagnosis of disease and revealed heterogeneity in the responses of patient sera. The combination of the 58-, 57-, and 45-kDa proteins confirmed a diagnosis of paracoccidioidomycosis (87% of the cases).
authors.affiliation: Unité de Mycologie, Institut Pasteur, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Casotto
mesh_terms: D000946:Antigens, Fungal / Q000032:analysis*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007526:Isoelectric Point; D007700:Kinetics; D003904:Mitosporic Fungi / Q000276:immunology*; D009181:Mycoses / Q000276:immunology*; D010228:Paracoccidioides / Q000276:immunology*
chemical_list: D000946:Antigens, Fungal; D007074:Immunoglobulin G