Applicability of fixed brain slice for ultrastructural study.
A new method is described to study the dendritic morphology and axonal collaterals from fixed slice preparations. Either rhodamine or fluorescein conjugated horseradish peroxidase was used to fill single projecting neurons intracellularly. The identification of projecting neurons was labelled first with rhodamine-coated latex microspheres. After the diaminobenzidine reaction, the filling of detailed morphological characteristics can be examined at both the light and electron microscopic levels. The advantages of this technique are discussed.
Lin
C S
CS
Department of Physiology and Biophysics, Hahnemann University, Philadelphia, PA 19102.
D000818:Animals; D001921:Brain / Q000648:ultrastructure*; D003712:Dendrites / Q000648:ultrastructure*; D005260:Female; D019793:Fluorescein; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006735:Horseradish Peroxidase; D066298:In Vitro Techniques; D008854:Microscopy, Electron / Q000379:methods*; D010544:Peroxidases; D051381:Rats; D012235:Rhodamines; D014966:Xanthenes
D005452:Fluoresceins; D005456:Fluorescent Dyes; D012235:Rhodamines; D014966:Xanthenes; D006735:Horseradish Peroxidase; D010544:Peroxidases; D019793:Fluorescein