Heat stress induced enhancement of heat shock protein gene activity in the honey bee (Apis mellifera).
We employed in vitro translation of mRNA and product separation using SDS-PAGE to examine the heat-shock response of the worker honey bee. Increases in the levels of 6 translatable RNA populations were observed following heat stress. The greatest response was observed among bees aged 9 days. Slight levels of induction of 70 and 82 kDa heat shock proteins were evident among bees taken directly from the colony.
Severson
D W
DW
Department of Veterinary Science, University of Wisconsin, Madison 53706.
Erickson
E H
EH
Williamson
J L
JL
Aiken
J M
JM
D000367:Age Factors; D000818:Animals; D001516:Bees / Q000235:genetics*; D015180:Electrophoresis, Gel, Two-Dimensional; D015870:Gene Expression; D006360:Heat-Shock Proteins / Q000235:genetics*; D006358:Hot Temperature; D008970:Molecular Weight; D012333:RNA, Messenger / Q000235:genetics
D006360:Heat-Shock Proteins; D012333:RNA, Messenger