Gallbladder CCK receptors: species differences in glycosylation of similar protein cores.
Receptors for cholecystokinin (CCK) on gallbladder muscularis smooth muscle have different apparent sizes in man (Mr = 85,000-95,000) and cow (Mr = 70,000-85,000). In this work, these receptors were demonstrated to represent N-linked complex glycoproteins with Mr = 43,000 protein cores, based on lectin-affinity chromatography and the deglycosylation of bands affinity labeled with 125I-D-Tyr-Gly-[(Nle28,31, pNO2-Phe33)CCK-26-33] using neuraminidase, O-glycanase and endoglycosidases H and F. Similarities in the core proteins were further demonstrated by Staphylococcus aureus V8 protease peptide mapping, in which both proteins yielded similar fragment patterns. Thus, gallbladder CCK receptors present in man and cow are both N-linked complex glycoproteins, with different carbohydrate domains and similar protein cores.
Schjoldager
B
B
Gastroenterology Research Unit, Mayo Clinic, Rochester, MN 55905.
Molero
X
X
Miller
L J
LJ
D000345:Affinity Labels; D000818:Animals; D001345:Autoradiography; D002417:Cattle; D002462:Cell Membrane / Q000032:analysis; D002846:Chromatography, Affinity; D005704:Gallbladder / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis*; D006031:Glycosylation; D006801:Humans; D009130:Muscle, Smooth / Q000032:analysis*; D010449:Peptide Mapping; D011949:Receptors, Cholecystokinin / Q000032:analysis*; D012697:Serine Endopeptidases
D000345:Affinity Labels; D006023:Glycoproteins; D011949:Receptors, Cholecystokinin; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase