The murine situs inversus viscerum (iv) gene responsible for visceral asymmetry is linked tightly to the Igh-C cluster on chromosome 12.
The iv gene controls left-right determination during murine organogenesis. To map this gene, we analyzed backcross progeny produced by mating (C57BL/6J X MEV/Ty)F1-iv/+heterozygotes to C57BL/6J-iv homozygotes. Hybridization of a murine ecotropic virus probe and several homeotic box gene probes coupled with analysis of dominant visible markers enabled us to exclude the iv locus from much of the mouse genome. Spurred by a recent report that mapped the iv gene to mouse chromosome 12 which was not excluded by our previous work, we used the polymerase chain reaction on our larger cohort to determine that the iv gene is indeed linked tightly to the Igh-C locus on this chromosome: we observed 0/156 recombinants between the iv and Igh-C loci. Combining data from the two studies demonstrates that the murine iv gene is close (1/201 recombinants) to the Igh-C cluster on chromosome 12.
Hanzlik
A J
AJ
Department of Pediatrics, Howard Hughes Medical Institute, University of Michigan School of Medicine, Ann Arbor 48109.
Binder
M
M
Layton
W M
WM
Rowe
L
L
Layton
M
M
Taylor
B A
BA
Osemlak
M M
MM
Richards
J E
JE
Kurnit
D M
DM
Stewart
G D
GD
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002875:Chromosomes; D003433:Crosses, Genetic; D005796:Genes; D008040:Genetic Linkage; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D012857:Situs Inversus / Q000235:genetics*