Modifications to commercial oxalate oxidase based determination of urinary oxalate: a method suitable for routine clinical analysis.
A commercially available assay (Sigma) for urinary oxalates based on the oxalate oxidase/peroxidase catalyzed reaction produced, in our hands, recoveries of added oxalate that varied from patient to patient. A modification of the assay is reported that involves the chromatographic separation of interfering substances, such as ascorbate and uric acid, from oxalate prior to analysis. With the modification recoveries of added oxalate were essentially complete and showed less patient-to-patient variability. Correlation with an alternate procedure was greatly improved.
Mazzuchin
A
A
Department of Pathology, Laurentian Hospital, Sudbury, Ontario, Canada.
Michelutti
L
L
Falter
H
H
D006801:Humans; D010070:Oxalates / Q000652:urine*; D010088:Oxidoreductases; D013997:Time Factors
D010070:Oxalates; D010088:Oxidoreductases; C020598:oxalate oxidase