[Detection of anti-sperm antibodies on the surface of live spermatozoa by the method of flow cytometry].
Detection of antisperm antibodies is an important step of the diagnosis of infertility. In present study we conducted comparative studies with the detection of antisperm antibodies in IgG, IgA and IgM classes on the surface of living spermatozoa by flow cytometry and with Mixed Agglutination Reaction and Sperm immobilization. Our data show that flow cytometry may be used to verify that immunoglobulins are on the surface of living spermatozoa, either retrieved directly from an ejaculate or following exposure to cervical mucus or serum and to determine their isotype, proportion of sperm which bound antibody and quantity of immunoglobulins bound to cell surface.
Nikolaeva
M A
MA
Ter-Avanesov
G V
GV
Terekhina
L N
LN
Ishenichnikova
T Ia
TIa
Sukhikh
G T
GT
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis* / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology; D007075:Immunoglobulin M / Q000032:analysis* / Q000276:immunology; D007248:Infertility, Male / Q000175:diagnosis* / Q000276:immunology; D008297:Male; D051379:Mice; D013094:Spermatozoa / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M
Vyiavlenie antispermal'nykh antitel na poverkhnosti zhivykh spermatozoidov metodom protochnoĭ tsitofluorometrii.