Hepatocellular cancer development in spleen of syngenic rats after transfer of potential precursor hepatocytes.
An in vitro-in vivo approach was used to examine the effect of selected cell proliferation on the progression to cancer. Liver cells isolated from rat donors with nodular livers were kept for 3 h in vitro, transferred to spleens and livers of syngeneic recipients and assessed at 4 and 10 months for evidence of cancer. Ten months after transfer of one million nodular liver cells (40% were GST-P + hepatocytes) to each of 50 recipients, 13 rats developed 21 hepatocellular cancers in their spleens (11 cancers) and livers. The latency to cancer was shortened by exposure to 2-acetylaminofluorene of the recipient rats at the time of hepatocyte transfer.
Eriksson
L C
LC
Department of Pathology, University of Toronto, Ontario, Canada.
Lee
G
G
Cameron
R
R
D015073:2-Acetylaminofluorene; D000818:Animals; D002455:Cell Division; D004052:Diethylnitrosamine; D005982:Glutathione Transferase / Q000378:metabolism; D006498:Hepatectomy; D008113:Liver Neoplasms / Q000209:etiology / Q000473:pathology*; D008114:Liver Neoplasms, Experimental / Q000209:etiology*; D009368:Neoplasm Transplantation; D011230:Precancerous Conditions / Q000473:pathology*; D051381:Rats; D011916:Rats, Inbred F344; D013154:Spleen / Q000473:pathology; D013997:Time Factors
D004052:Diethylnitrosamine; D015073:2-Acetylaminofluorene; D005982:Glutathione Transferase