[Heterogeneity of polyadenylation site of mRNA coding for human serum albumin].
Human fetal liver cDNA was cloned in pBR322 vector by dG:dC-tailing method. The cDNA library was screened for liver-specific clones by means of differential hybridization. Human fetal liver and human kidney cDNAs were used as hybridization probes. Application of this procedure revealed twenty five liver-specific clones among about one thousand recombinants analysed. These clones represent cDNAs corresponding abundant mRNAs. Eighteen clones were identified as encoding serum albumin. Two different mRNA polyadenylation sites were found in four sequenced plasmids. Cleavage/polyadenylation site in two plasmids, pHA1 and pHA12, is situated fifteen nucleotides downstream the AATAAA signal; in two other plasmids, pHA8 and pHA25, this site is ten nucleotides downstream the same signal.
Dmitrenko
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Kavsan
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D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D015723:Gene Library; D006801:Humans; D008099:Liver / Q000196:embryology / Q000378:metabolism; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011061:Poly A / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012709:Serum Albumin / Q000235:genetics*
D012333:RNA, Messenger; D012709:Serum Albumin; D011061:Poly A; D004247:DNA
Geterogennost' saĭta poliadenilirovaniia mRNK, kodiruiushcheĭ chelovecheskiĭ syvorotochnyĭ al'bumin.