title: Autoantibodies against different histone H1 subtypes in systemic lupus erythematosus sera.
abstract: The H1 histones represent the most heterogenous class of histone proteins. In this study, we analyzed the specificity of human antibodies against 6 H1 subtypes. H1 histones from rat organs were separated by reverse-phase high performance liquid chromatography and used as antigens in immunoblotting experiments. Sera containing anti-histone H1 antibodies were obtained from patients with systemic lupus erythematosus. Of the 9 sera tested, 2 reacted with only 1 H1 subtype. The other sera recognized different combinations of H1 subtypes. Only 1 serum reacted with all 6 H1 subtypes. Histones H1.5 and H1.1 were the subtypes most frequently recognized by the human autoantibodies. Our data indicate that human anti-H1 antibodies represent a heterogenous population, directed mainly against epitopes localized in the variable region of the H1 molecule.
authors.affiliation: Institute of Tumor Biology-Cancer Research, University of Vienna, Austria.
authors.forename: J
authors.initials: J
authors.lastname: Wesierska-Gadek
authors.forename: E
authors.initials: E
authors.lastname: Penner
authors.forename: H
authors.initials: H
authors.lastname: Lindner
authors.forename: E
authors.initials: E
authors.lastname: Hitchman
authors.forename: G
authors.initials: G
authors.lastname: Sauermann
mesh_terms: D000918:Antibody Specificity; D001323:Autoantibodies / Q000276:immunology*; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D008180:Lupus Erythematosus, Systemic / Q000097:blood / Q000276:immunology*
chemical_list: D001323:Autoantibodies; D006657:Histones