Assessment of electrophoretic mobility changes of human erythrocytes by free flow electrophoresis after in vitro and in vivo irradiation.
Single cell measurements of electrophoretic mobility showed a linear dose-response of human erythrocytes after exposure to ionizing radiation. To establish a biological indicator- or dosimeter-system for application to dose-estimations after accidental exposures, we tried to measure dose dependent EPM-changes by the free flow electrophoresis technique, a rapid and efficient method which supplies the capacity needed for application. The in vitro irradiated erythrocytes of most donors showed an oscillating dose-response but erythrocytes from several other individuals in a contradictory manner whereby the EPM of erythrocytes from radiotherapy patients increased linearly with the amount of administered dose. However, the wide interindividual EPM-range of unirradiated erythrocytes inhibits the application of this technique in biological dosimetry.
Schreiber
G A
GA
Institut für Strahlenhygiene des Bundesgesundheitsamtes, Neuherberg/München, F.R.G.
Spiegelberg
A
A
Butkowskyj-Walkiw
T
T
Bögl
K W
KW
D004307:Dose-Response Relationship, Radiation; D004586:Electrophoresis; D004912:Erythrocytes / Q000528:radiation effects*; D006801:Humans; D066298:In Vitro Techniques; D011874:Radiometry / Q000379:methods*