Identification of an ascorbic acid metabolite among "uremic middle molecules".
Among uremic toxins in the middle molecular mass range, 1H, 13C-nuclear magnetic resonance, ultraviolet spectrometry, and chromatographic analyses allow identification of the main component of the so-called "2-5-3 fraction" as ascorbic acid 2-sulfate, a conjugated metabolite of ascorbic acid. We previously (Clin Nephrol 1986;25:212-8) showed an inhibitory effect of the 2-5-3 fraction on microtubule formation. Therefore, we tested the action of ascorbic acid 2-sulfate and its synthetized enantiomers on tubulin polymerization. Because these molecules did not exert any inhibitory effect, we hypothesize that the 2-5-3 fraction is a mixture of compounds in which only a very low quantity of the inhibitory factor is present.
Gallice
P M
PM
Laboratoire de Biophysique, Faculté de Pharmacie, Marseille, France.
Monti
J P
JP
Braguer
D L
DL
Monti
H M
HM
Elsen
R E
RE
Berland
Y F
YF
Crevat
A D
AD
D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D004735:Energy Transfer; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008870:Microtubules / Q000378:metabolism; D015394:Molecular Structure; D013056:Spectrophotometry, Ultraviolet; D014404:Tubulin / Q000187:drug effects / Q000502:physiology; D014511:Uremia / Q000378:metabolism*
D014404:Tubulin; C001956:ascorbic acid 2-sulfate; D001205:Ascorbic Acid