Identification of the second major allergen of Japanese cedar pollen.
We isolated and characterized the second major allergen (Cry j II) from Japanese cedar pollen. We found that most patients with this pollinosis had IgE antibody to this protein in addition to IgE antibody to Cry j I; however, some sera reacted only with Cry j I or Cry j II. IgE-ELISA inhibition studies revealed that Cry j I and Cry j II had no cross-allergenicity. Cry j II did not react with anti-Cry j I monoclonal antibodies. In SDS-PAGE under a non-reducing condition, Cry j II showed a band at the 37 kDa position, compared with the 45-50 kDa bands of Cry j I. N-terminal amino acid sequence of Cry j II was completely different from that of Cry j I.
Sakaguchi
M
M
Department of Microbiology, Institute of Public Health, Tokyo, Japan.
Inouye
S
S
Taniai
M
M
Ando
S
S
Usui
M
M
Matuhasi
T
T
D000485:Allergens; D000595:Amino Acid Sequence; D052179:Antigens, Plant; D002845:Chromatography; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006967:Hypersensitivity / Q000453:epidemiology / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology; D007564:Japan; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000276:immunology / Q000302:isolation & purification*; D011058:Pollen / Q000032:analysis* / Q000276:immunology*; D014197:Trees
D000485:Allergens; D052179:Antigens, Plant; C065290:Cry j I protein, Cryptomeria japonica; C065291:Cry j II protein, Cryptomeria japonica; D010940:Plant Proteins; D007073:Immunoglobulin E