Gene transfer, expression and inheritance of pRSV-rainbow trout-GH cDNA in the common carp, Cyprinus carpio (Linnaeus).
A recombinant plasmid containing the Rous sarcoma virus-long terminal repeat (RSV-LTR) promoter linked to rainbow trout (Salmo gairdneri) growth hormone (GH) cDNA was microinjected into fertilized carp eggs. Genomic DNA extracted from pectoral fin of individual presumptive transgenic fish was analyzed by dot blot and Southern blot hybridization, using the RSV-LTR and/or the GH cDNA sequences as probes. Out of 365 presumptive transgenic fish analyzed, 20 individuals were found to contain pRSV-rtGH-cDNA sequence in the genomic DNA. Expression of the trout GH polypeptide was detected by immunobinding assay in the red blood cells of nine transgenic fish tested. The level of expression, however, varied among the transgenics and could not be correlated with exogenous DNA copy number. Although there was considerable variation in the sizes of the transgenic fish, those microinjected during the one-cell stage were (P less than 0.05) 22% larger, on the average, than their sibling controls. A randomly selected fraction of the progeny derived from crosses between transgenic males and non-transgenic females inherited the foreign DNA. These transgenic progeny grew faster (P less than 0.05) than their non-transgenic siblings.
Zhang
P J
PJ
Hopkins Marine Station, Stanford University, Pacific Grove, California.
Hayat
M
M
Joyce
C
C
Gonzalez-Villaseñor
L I
LI
Lin
C M
CM
Dunham
R A
RA
Chen
T T
TT
Powers
D A
DA
D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D001835:Body Weight; D002347:Carps / Q000196:embryology* / Q000235:genetics; D003530:Cyprinidae / Q000196:embryology*; D004247:DNA / Q000032:analysis; D004625:Embryo, Nonmammalian / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D015870:Gene Expression; D006062:Gonadotropins / Q000235:genetics*; D008845:Microinjections; D010957:Plasmids; D012487:Salmonidae / Q000235:genetics*; D014162:Transfection; D014337:Trout / Q000235:genetics*
D006062:Gonadotropins; D004247:DNA