title: Bovine osteogenic protein is composed of dimers of OP-1 and BMP-2A, two members of the transforming growth factor-beta superfamily.
abstract: A bone-inductive protein has been purified from bovine bone and designated as osteogenic protein (OP). The purified OP induces new bone at less than 5 ng with half-maximal bone differentiation activity at about 20 ng/25 mg of matrix implant in a subcutaneous bone induction assay. The purified osteogenic protein is composed of disulfide-linked dimers that migrate on sodium dodecyl sulfate gels as a diffuse band with an apparent molecular weight of 30,000. Upon reduction, the dimers yield two subunits that migrate with molecular weights of 18,000 and 16,000. Both subunits are glycosylated. After chemical or enzymatic deglycosylation, the dimers migrate as a diffuse 27-kDa band that upon reduction yields two polypeptides that migrate at 16 kDa and 14 kDa, respectively. The carbohydrate moiety does not appear to be essential for biological activity since the deglycosylated proteins are capable of inducing bone formation in vivo. Amino acid sequences of peptides generated by proteolytic digestion show that the subunits are distinct but related members of the transforming growth factor-beta super-family. The 18-kDa subunit is the protein product of the bovine equivalent of the human OP-1 gene and the 16-kDa subunit is the protein product of the bovine equivalent of the human BMP-2A gene.
authors.affiliation: Creative BioMolecules, Inc., Hopkinton, Massachusetts 01748.
authors.forename: T K
authors.initials: TK
authors.lastname: Sampath
authors.forename: J E
authors.initials: JE
authors.lastname: Coughlin
authors.forename: R M
authors.initials: RM
authors.lastname: Whetstone
authors.forename: D
authors.initials: D
authors.lastname: Banach
authors.forename: C
authors.initials: C
authors.lastname: Corbett
authors.forename: R J
authors.initials: RJ
authors.lastname: Ridge
authors.forename: E
authors.initials: E
authors.lastname: Ozkaynak
authors.forename: H
authors.initials: H
authors.lastname: Oppermann
authors.forename: D C
authors.initials: DC
authors.lastname: Rueger
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001857:Bone Matrix / Q000378:metabolism / Q000637:transplantation; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D001842:Bone and Bones / Q000378:metabolism; D002417:Cattle; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006031:Glycosylation; D006133:Growth Substances / Q000235:genetics; D046911:Macromolecular Substances; D008970:Molecular Weight; D005810:Multigene Family; D010012:Osteogenesis; D011506:Proteins / Q000235:genetics* / Q000302:isolation & purification; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D016212:Transforming Growth Factor beta; D015291:Transforming Growth Factors / Q000235:genetics*
chemical_list: C525819:BMP7 protein, human; C525821:Bmp7 protein, rat; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D006133:Growth Substances; D046911:Macromolecular Substances; D011506:Proteins; D016212:Transforming Growth Factor beta; D015291:Transforming Growth Factors; D000469:Alkaline Phosphatase