title: Superior myocardial preservation with modified UW solution after prolonged ischemia in the rat heart.
abstract: Cardiac transplantation remains constrained by poor graft tolerance of prolonged cold ischemia. University of Wisconsin solution has remarkably extended ischemic preservation in pancreas, kidney, and liver transplantation. To assess its efficacy in cardiac preservation, modified University of Wisconsin solution flush and storage were tested against St. Thomas' cardioplegia flush and normal saline solution storage after six hours of ischemia at 0 degrees C in 46 isolated rat hearts. After ischemia, groups were compared before and after reperfusion. After ischemia but before reperfusion, University of Wisconsin solution hearts had significantly less tissue water (3.8%), superior tissue sodium, potassium, calcium, and magnesium profiles, and elevated adenosine and inosine levels, and tended toward better histological preservation. After reperfusion, University of Wisconsin solution more effectively preserved left ventricular compliance (75% versus 35% of baseline), developed pressure (71% versus 45% of baseline), histological integrity, and tissue potassium and calcium profiles than St. Thomas' solution. The University of Wisconsin solution provided superior preservation of systolic and diastolic ventricular function, tissue histology, tissue water, and tissue electrolytes than did St. Thomas' cardioplegia and normal saline solution storage in this experimental model, and might result in improved graft tolerance of ischemia in clinical cardiac transplantation.
authors.affiliation: Department of Surgery, Medical College of Virginia, Richmond.
authors.forename: T
authors.initials: T
authors.lastname: Yeh
authors.forename: S A
authors.initials: SA
authors.lastname: Hanan
authors.forename: D E
authors.initials: DE
authors.lastname: Johnson
authors.forename: I M
authors.initials: IM
authors.lastname: Rebeyka
authors.forename: A S
authors.initials: AS
authors.lastname: Abd-Elfattah
authors.forename: K F
authors.initials: KF
authors.lastname: Lee
authors.forename: A S
authors.initials: AS
authors.lastname: Wechsler
mesh_terms: D000227:Adenine Nucleotides / Q000032:analysis / Q000378:metabolism; D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D001639:Bicarbonates / Q000627:therapeutic use; D001794:Blood Pressure / Q000502:physiology; D001834:Body Water / Q000032:analysis; D002118:Calcium / Q000032:analysis; D002122:Calcium Chloride / Q000627:therapeutic use; D002314:Cardioplegic Solutions / Q000627:therapeutic use; D015925:Cryopreservation; D005978:Glutathione; D006321:Heart / Q000502:physiology; D016027:Heart Transplantation; D007328:Insulin; D007511:Ischemia; D008274:Magnesium / Q000627:therapeutic use; D009200:Myocardial Contraction / Q000502:physiology; D009206:Myocardium / Q000032:analysis / Q000473:pathology; D019188:Organ Preservation Solutions; D011188:Potassium / Q000032:analysis; D011189:Potassium Chloride / Q000627:therapeutic use; D011887:Raffinose; D051381:Rats; D011919:Rats, Inbred Strains; D012965:Sodium Chloride / Q000627:therapeutic use; D012996:Solutions / Q000627:therapeutic use*; D013054:Spectrophotometry, Atomic; D014021:Tissue Preservation
chemical_list: D000227:Adenine Nucleotides; D001639:Bicarbonates; D002314:Cardioplegic Solutions; D007328:Insulin; D019188:Organ Preservation Solutions; D012996:Solutions; C041711:St. Thomas' Hospital cardioplegic solution; C055206:University of Wisconsin-lactobionate solution; D012965:Sodium Chloride; D000493:Allopurinol; D011189:Potassium Chloride; D005978:Glutathione; D008274:Magnesium; D000241:Adenosine; D002122:Calcium Chloride; D011887:Raffinose; D011188:Potassium; D002118:Calcium