Determination of malondialdehyde with chemical derivatization into the pyrimidine compound and HPLC.
A new HPLC method to determine malondialdehyde (MDA) was developed. Malondialdehyde was reacted with alpha-N-benzoyl-L-arginine ethyl ester and converted into alpha-N-benzoyl-delta-N-(2-pyrimidinyl)-L-ornithine ethyl ester, which is sufficiently hydrophobic to allow us its specific determination utilizing a reversed-phase C18 column at the level of 1 pmol.
Kishida
E
E
Department of Life and Health Sciences, Hyogo University of Teacher Education, Japan.
Oribe
M
M
Mochizuki
K
K
Kojo
S
S
Iguchi
H
H
D001120:Arginine / Q000031:analogs & derivatives; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D008314:Malonates / Q000032:analysis*; D008315:Malondialdehyde / Q000032:analysis*; D011743:Pyrimidines
D008314:Malonates; D011743:Pyrimidines; D008315:Malondialdehyde; D001120:Arginine; C009727:benzoylarginine ethyl ester