Human T-cell leukemia virus type-I-infected T-cell lines scarcely produce p56lck, whether or not they express lck mRNA.
We have previously reported that lck mRNA (a lymphocyte-specific protein tyrosine kinase gene) is absent in human T-cell leukemia virus type I (HTLV-I)-infected interleukin-2(IL-2)-independent T-cell lines, while HTLV-I-negative T-cell lines and HTLV-I-positive IL-2-dependent ones express a large amount of lck mRNA. To further investigate the levels of lck expression, we prepared rabbit anti-Lck antiserum directed against the synthetic oligopeptide of 32 amino acids corresponding to the carboxy terminus of this gene product, p56lck. Using this antiserum, we show that HTLV-I-positive T-cell lines, whether they are IL-2-dependent or not, scarcely express p56lck. In other words, IL-2-dependent HTLV-I-positive T-cell lines seldom produce p56lck in spite of high expression of lck mRNA. Absence of p56lck is suspected of playing an important role in malignant transformation of HTLV-I-infected T-cells.
Oh-Hori
N
N
Department of Immunology, Kyushu University, Fukuoka, Japan.
Koga
Y
Y
Yoshida
H
H
Morita
M
M
Kimura
G
G
Nomoto
K
K
D015152:Blotting, Northern; D002460:Cell Line; D002478:Cells, Cultured; D015967:Gene Expression Regulation, Viral / Q000235:genetics* / Q000276:immunology; D015490:HTLV-I Infections / Q000235:genetics* / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D009856:Oncogene Proteins, Viral / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D014176:Protein Biosynthesis; D011505:Protein-Tyrosine Kinases / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D009856:Oncogene Proteins, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; D011505:Protein-Tyrosine Kinases; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck)