A54145, a new lipopeptide antibiotic complex: discovery, taxonomy, fermentation and HPLC.
A54145 is a complex of new lipopeptide antibiotics that inhibits Gram-positive bacteria and acts as a growth promotant for broiler chicks. Eight factors; A, B, C, D, E, F, A1 and B1; have been isolated and characterized. They contain four similar peptide nuclei, each of which is acylated with either an 2-decanoyl, n-decanoyl, or undecanoyl side chain. Taxonomic studies ascertained that the producing microorganism was a strain of Streptomyces fradiae. Fermentation studies determined that superior antibiotic yields were obtained in stirred bioreactors in a soybean flour-molasses medium employing a continuous glucose feed. These findings, interwoven with the selection of hyper-productive mutants, increased fermentation yields from less than 50 micrograms/ml to more than 1 mg/ml. An analytical HPLC system was developed for the identification and subsequent quantitation of each factor of the A54145 complex.
Boeck
L D
LD
Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, IN 46285.
Papiska
H R
HR
Wetzel
R W
RW
Mynderse
J S
JS
Fukuda
D S
DS
Mertz
F P
FP
Berry
D M
DM
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000096:biosynthesis*; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D008074:Lipoproteins / Q000032:analysis / Q000096:biosynthesis; D008969:Molecular Sequence Data; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification / Q000378:metabolism*
C065208:A54145; D000900:Anti-Bacterial Agents; D008074:Lipoproteins