Biochemical effects of luxabendazole on Trichinella spiralis.
Biochemical changes produced by luxabendazole in muscle-stage Trichinella spiralis larvae consisted of a decrease in free glucose and glycogen levels (46.71% and 35.66%, respectively) after in vivo treatment, slight in vitro inhibition of fumarate reductase activity (24.15%) and, finally, inhibition of [3H]-colchicine-tubulin binding, which was found to be of a competitive nature, with an inhibition constant (Ki) of 0.9 x 10(-7) M. In a parallel study, luxabendazole did not appear to be inhibitory to [3H]-colchicine binding to pig-brain tubulin.
Criado-Fornelio
A
A
Parasitology and Microbiology Department, Faculty of Pharmacy, University of Alcalá de Henares, Madrid, Spain.
de Armas-Serra
C
C
Jimenez-Gonzalez
A
A
Casado-Escribano
N
N
Rodriguez-Caabeiro
F
F
D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D001562:Benzimidazoles / Q000494:pharmacology*; D001667:Binding, Competitive; D002219:Carbamates / Q000494:pharmacology*; D003078:Colchicine / Q000378:metabolism; D014234:Trichinella / Q000187:drug effects* / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism
D000871:Anthelmintics; D001562:Benzimidazoles; D002219:Carbamates; D014404:Tubulin; C058092:luxabendazole; D003078:Colchicine