Chronic carbon tetrachloride and phospholipase D hepatotoxicity in rat: in vivo 1H magnetic resonance, total lipid analysis, and histology.
Magnetic resonance (MR) imaging, localized in vivo proton spectroscopy, and T1 relaxation measurements were obtained from the livers of rats treated chronically with carbon tetrachloride and phospholipase D. The MR data correlated well with lipid changes measured biochemically and histologically. MR images appeared generally hyperintense during fatty infiltration, changing to hypointense mottling during cirrhosis. Water T1 relaxation times showed no statistically significant change at any time during the experiments from the control value of 908 ms (SE = 42 ms). Minor changes in lipid T1 values with time were noted. The average lipid T1 curve demonstrated a linear relation with time (r = 0.81), increasing from the control value of 283 ms (+/- 16 ms) to 365 ms (+/- 53 ms) at the end of the third week and decreasing slightly through the end of the experiment. Water-suppressed in vivo spectra showed quantitative changes in liver lipids which correlated well with the biochemical and histologic analysis. From the MR images and spectroscopy results it was possible to distinguish early fatty liver from more advanced cirrhosis.
Hazle
J D
JD
Department of Radiology, University of Texas Medical School, Houston 77030.
Narayana
P A
PA
Dunsford
H A
HA
D000818:Animals; D002252:Carbon Tetrachloride Poisoning / Q000378:metabolism / Q000473:pathology*; D005234:Fatty Liver / Q000378:metabolism / Q000473:pathology; D006859:Hydrogen; D008055:Lipids / Q000032:analysis*; D008099:Liver / Q000032:analysis / Q000187:drug effects* / Q000473:pathology; D008106:Liver Cirrhosis, Experimental / Q000378:metabolism / Q000473:pathology; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D010739:Phospholipase D / Q000633:toxicity*; D010740:Phospholipases / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains
D008055:Lipids; D006859:Hydrogen; D010740:Phospholipases; D010739:Phospholipase D