title: Antibody to cytotoxin in infection by Helicobacter pylori.
abstract: Gastrointestinal disease and colonization by Helicobacter pylori were determined in 36 asymptomatic volunteers and 30 symptomatic individuals undergoing endoscopy and biopsy. Serum antibody immunoglobulin G (IgG) and IgA to H. pylori were measured by enzyme-linked immunosorbent assay. Serum antibody to a cytotoxin produced by H. pylori was detected with a neutralization assay. Serum IgG was 95% predictive of infection by H. pylori, and serum IgA was 88% predictive. Antibody to the cytotoxin was detected in 12 of 18 infected individuals. Antibody to the cytotoxin was a highly specific (96%), but not a very sensitive (67%), indicator of infection by H. pylori. The neutralization assay was 87% predictive of infection. These data confirm the diagnostic value of serum antibody to H. pylori for the detection of infection. The toxin-neutralizing activity of sera from individuals infected with H. pylori suggests that the cytotoxin is produced in vivo. It may therefore contribute to disease associated with H. pylori.
authors.affiliation: Miami Valley Laboratories, Procter & Gamble Company, Cincinnati, Ohio 45239.
authors.forename: R D
authors.initials: RD
authors.lastname: Leunk
authors.forename: M A
authors.initials: MA
authors.lastname: Ferguson
authors.forename: D R
authors.initials: DR
authors.lastname: Morgan
authors.forename: D E
authors.initials: DE
authors.lastname: Low
authors.forename: A E
authors.initials: AE
authors.lastname: Simor
mesh_terms: D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000032:analysis*; D002167:Campylobacter / Q000276:immunology; D002169:Campylobacter Infections / Q000276:immunology*; D003603:Cytotoxins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005767:Gastrointestinal Diseases / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009500:Neutralization Tests
chemical_list: D000907:Antibodies, Bacterial; D003603:Cytotoxins; D007074:Immunoglobulin G