[The surface charge of NKLy ascitic tumor cells differing in their proliferative activity, in their place in the cell cycle and in their ploidy].
The NKLy ascitic tumor cells in the stationary phase of growth were fractionated by velocity sedimentation method. Cells from the obtained fractions were characterized by measurements of DNA contents and 3H-thymidine incorporation. The surface properties of the cells from five fractions, differing in proliferative capacity, stage of the cell cycle and ploidy were considered using cell electrophoresis, two polymer aqueous phase system and Alcian blue sorption. A correlation between electrophoretic mobility and cell partition constant for these fractions has been obtained. No correlation was found between these parameters and dye absorption. The surface charge of the cells from G0/G1 and S fractions was higher than that of other cells. The polyploid NKLy cells demonstrated a lower surface charge. The surface properties of the tumor cells differing in proliferative capacity, stage of the cell cycle and ploidy are discussed.
Slivinskiĭ
G G
GG
Magda
I N
IN
D000423:Alcian Blue; D000818:Animals; D002453:Cell Cycle; D004273:DNA, Neoplasm / Q000032:analysis; D004586:Electrophoresis; D008223:Lymphoma / Q000032:analysis / Q000473:pathology*; D008564:Membrane Potentials; D051379:Mice; D011003:Ploidies; D013499:Surface Properties; D014407:Tumor Cells, Cultured / Q000032:analysis / Q000473:pathology
D004273:DNA, Neoplasm; D000423:Alcian Blue
Poverkhnostnyĭ zariad kletok astsitnoĭ opukholi NKLy, razlichaiushchikhsia proliferativnoĭ aktivnost'iu, polozheniem v kletochnom tsikle i ploidnost'iu.