Determination of cerebrospinal fluid production rate using a push-pull perfusion procedure in the conscious rat.
The inulin dilution technique was used to determine the cerebrospinal fluid (CSF) production rate by means of a push-pull cannula implanted into a lateral ventricle. Artificial CSF containing trace amount of 3H-inulin was perfused for 2 h in conscious rats. 3H-inulin in the effluent reached a plateau level depending on the CSF production rate. The control lateroventricular CSF production was 0.98 microliters/min. Production was reduced to 0.34 microliters/min during a perfusion with acetazolamide (1 mM), a carbonic anhydrase inhibitor.
Baudrie
V
V
Laboratoire de Pharmacologie, CNRS I 61670, Faculté de Médecine Necker-Enfants Malades, Paris, France.
Roullet
J B
JB
Goureau
Y
Y
Chaouloff
F
F
Elghozi
J L
JL
D000086:Acetazolamide / Q000494:pharmacology; D000818:Animals; D002404:Catheterization; D002552:Cerebral Ventricles / Q000378:metabolism; D002555:Cerebrospinal Fluid / Q000502:physiology*; D007444:Inulin / Q000493:pharmacokinetics; D008297:Male; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014316:Tritium
D014316:Tritium; D007444:Inulin; D000086:Acetazolamide