title: [Successful use in horses of deep-frozen semen specimens stored for 18 years].
abstract: In 1970 semen from a Haflinger-stallion was frozen by the pellet method. 18 years later semen samples were used to inseminate 4 mares. Inseminations were performed shortly after ovulation with a total number of motile spermatozoa between 150 and 636 x 10(6), the percentage of motile spermatozoa being 20% to 40%. Three mares conceived after a single insemination, one mare got pregnant after 4 inseminations during 3 oestrous periods. Meanwhile, 3 foals were born and one of the mares is still pregnant. The results demonstrate that long-term storage of frozen semen in liquid nitrogen does not impair its fertilizing capacity.
authors.affiliation: Gynäkologischen und Ambulatorischen Tierklinik der Universität München.
authors.forename: J
authors.initials: J
authors.lastname: Braun
authors.forename: W
authors.initials: W
authors.lastname: Schefels
authors.forename: R
authors.initials: R
authors.lastname: Stolla
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000662:veterinary*; D005260:Female; D007315:Insemination, Artificial / Q000662:veterinary*; D008297:Male; D011247:Pregnancy; D012661:Semen / Q000502:physiology*; D013081:Sperm Motility; D013997:Time Factors
vernacular_title: Erfolgreicher Einsatz von 18 Jahre gelagerten Tiefgefrier-Samenproben beim Pferd.