Counter-regulatory effects of phosphatidic acid on protein kinase C activity in the presence of calcium and diolein.
Phosphatidic acid can replace phosphatidylserine in the activation of protein kinase C. However, in the presence of diolein, the addition of Ca2+ results in the inhibition of the enzyme. This phenomenon could lead to a negative feedback regulation of protein kinase C activity as a result of stimulation of the cycling of phosphatidylinositol.
Epand
R M
RM
Department of Biochemistry, McMaster University, Health Sciences Centre, Hamilton, Ontario, Canada.
Stafford
A R
AR
D000818:Animals; D001921:Brain / Q000201:enzymology; D002118:Calcium / Q000494:pharmacology*; D004075:Diglycerides / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D005989:Glycerides / Q000494:pharmacology*; D066298:In Vitro Techniques; D008823:Micelles; D010712:Phosphatidic Acids / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats
D004075:Diglycerides; D005989:Glycerides; D008823:Micelles; D010712:Phosphatidic Acids; D011493:Protein Kinase C; D002118:Calcium; C013965:diolein