Phospholipase C isozymes: structural and functional similarities.
Phospholipase C (PLC) is shown to comprise at least nine isoforms. These isoforms can be separated into three structurally related classes. Within a class the isozymes have similar enzymological properties. In the case of the PLC gamma class, both isoforms may be regulated by tyrosine phosphorylation. For PLC gamma 1 we show that the tyrosine phosphorylation sites are contained within the SH2/SH3 region or 'modulatory domain'. The overexpression of PLC gamma 1 in Rat-2 cells results in increased phosphatidylinositol breakdown in response to PDGF treatment, demonstrating that PLC gamma 1 mediates this response. We note that thrombin activates PLC gamma 1 in addition to other PLC isoforms.
Kriz
R
R
Genetics Institute, Cambridge, MA 02140.
Lin
L L
LL
Sultzman
L
L
Ellis
C
C
Heldin
C H
CH
Pawson
T
T
Knopf
J
J
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002460:Cell Line; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015723:Gene Library; D006801:Humans; D007295:Inositol Phosphates / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection; D010738:Type C Phospholipases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
D007295:Inositol Phosphates; D007527:Isoenzymes; D010982:Platelet-Derived Growth Factor; D004247:DNA; D010738:Type C Phospholipases; D002118:Calcium