title: Highly sensitive method for the determination of 1-methyl-1,2,3,4-tetrahydro-beta-carboline using combined capillary gas chromatography and negative-ion chemical ionization mass spectrometry.
abstract: A sensitive method was developed for the determination of deuterated and non-deuterated 1-methyl-1,2,3,4-tetrahydro-beta-carboline by combined capillary gas chromatography and negative-ion chemical ionization mass spectrometry. 1-Methyl-1,2,3,4-tetrahydro-beta-carboline was converted into a trifluoroacetyl derivative after pretreatment with fluorescamine and extraction with ethyl acetate. The derivative was separated by capillary gas chromatography and determined by selected-ion monitoring. In the determination, [3,3,4,4-2H4]-1-methyl-1,2,3,4-tetrahydro-beta-carboline was used as an internal standard. The method developed in this work was used for the determination of deuterated and non-deuterated 1-methyl-1,2,3,4-tetrahydro-beta-carboline in human urine samples collected before and after administration of [3,3-2H2]-L-tryptophan.
authors.affiliation: National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Hayashi
authors.forename: H
authors.initials: H
authors.lastname: Todoriki
authors.forename: Y
authors.initials: Y
authors.lastname: Iida
mesh_terms: D000328:Adult; D002243:Carbolines / Q000652:urine*; D003903:Deuterium; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D008297:Male; D012016:Reference Values; D014363:Tryptamines / Q000652:urine; D014364:Tryptophan / Q000378:metabolism*
chemical_list: D002243:Carbolines; D014363:Tryptamines; C009777:2-methyltryptoline; C030820:tryptamine; D014364:Tryptophan; D003903:Deuterium