Cytogenetics of porcine sperm chromosomes following IVF of zona-free hamster oocytes.
Porcine sperm chromosome karyotypes were obtained from two boars following heterologous in vitro fertilization (IVF) of zona-free hamster ova with ionophore-capacitated porcine spermatozoa. The karyotype yield per inseminated ovum was 9.3% (n = 1658), and there was no significant deviation from the expected 1:1 sex ratio (p greater than 0.5). Cytogenetic analysis revealed hyperhaploid (2.5%) and hypohaploid spermatozoa (1.9%). Spermatozoa with structural chromosome aberrations constituted 7.1% of the analyzed karyotypes, and these were predominantly chromosomal breaks, although acentric fragments were also found (2.6%).
Bird
J M
JM
Department of Microbiology, University College, Galway, Ireland.
Houghton
J A
JA
D000818:Animals; D002869:Chromosome Aberrations / Q000235:genetics*; D025063:Chromosome Disorders; D006224:Cricetinae; D005260:Female; D005307:Fertilization in Vitro; D007621:Karyotyping; D008297:Male; D008647:Mesocricetus; D013084:Sperm-Ovum Interactions / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology / Q000648:ultrastructure*; D013552:Swine