[The role of protein binding with single-stranded DNA (SSB-protein) in DNA replication in Ehrlich ascites carcinoma cells].
Possible involvement of the single-strand DNA-binding protein (SSB-protein) in DNA replication in Ehrlich ascite tumour (EAT) cells was studied. There was a direct correlation between the content of SSB-protein in chromatin and the intensity of replicative synthesis of DNA in various preparations of EAT in vitro and in vivo (the computed value of the correlation coefficient was equal to 0.9). It was shown that the addition of exogenous SSB-protein to permeable EAT cells increased the replicative synthesis. It was concluded that although eukaryotic SSB-proteins are not complete analogs of prokaryotic ones, they may participate in DNA replication in eukaryotic cells and, possibly, are intracellular regulators of proliferation.
Koterov
A N
AN
Novoradovskaia
N A
NA
Filippovich
I V
IV
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism; D004261:DNA Replication; D004273:DNA, Neoplasm / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D051379:Mice; D014407:Tumor Cells, Cultured / Q000378:metabolism
D002843:Chromatin; D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins
Uchastie belka, sviazyvaiushchegosia s odnonitevoĭ DNK (SSB-belka), v replikatsii DNK v kletkakh astsitnoĭ kartsinomy Erlikha.