title: Comparison of blood-based and asanguineous cardioplegic solutions administered at 4 degrees C. An ultrastructural morphometric study in the dog.
abstract: Although several studies have shown better myocardial preservation with blood-based than asanguineous cardioplegic solutions at myocardial temperatures above 15 degrees C, one might suspect that blood would become unsafe at lower temperatures because of increased oxygen-hemoglobin affinity and viscosity. We compared myocardial preservation in dogs subjected to 6 hours of aortic crossclamping and treated with modified Roe's asanguineous cardioplegic solution at 4 degrees C (group CA), blood cardioplegic solution at 4 degrees C (CB), or blood cardioplegic solution at 27 degrees C (WB, four dogs per group). Myocardial preservation was assessed by triphenyltetrazolium staining of whole hearts, and by analysis of ultrastructure and morphometric analysis of mitochondria in myocardial biopsies from three sites in each heart (left ventricle subepicardium and subendocardium and right ventricle). Tetrazolium staining showed no difference in preservation among the three treatment groups (no necrosis in any heart). For two of the three biopsy sites (left ventricular subepicardium and right ventricle), ultrastructural and morphometric analyses demonstrated signs of more severe subcellular injury in group CA than in CB (p = 0.013 to 0.004), whereas equivalent preservation with all treatments was observed in the left ventricular endocardial site. Functional recovery also appeared to be equivalent between treatments, to the extent that all dogs were successfully weaned from bypass after 20 minutes of reperfusion. We conclude that the safety and effectiveness of blood cardioplegia is not compromised by infusion at 4 degrees C compared with 27 degrees C and that myocardial preservation is not improved by using asanguineous cardioplegia instead of blood cardioplegia at 4 degrees C.
authors.affiliation: Department of Pathology, University of Toronto, Ontario, Canada.
authors.forename: R A
authors.initials: RA
authors.lastname: Axford-Gatley
authors.forename: G J
authors.initials: GJ
authors.lastname: Wilson
authors.forename: C M
authors.initials: CM
authors.lastname: Feindel
mesh_terms: D000818:Animals; D001011:Aorta / Q000601:surgery; D001769:Blood; D002314:Cardioplegic Solutions / Q000008:administration & dosage*; D003080:Cold Temperature; D003250:Constriction; D003327:Coronary Disease / Q000473:pathology; D004285:Dogs; D005947:Glucose / Q000008:administration & dosage; D006321:Heart / Q000187:drug effects*; D015636:Magnesium Chloride / Q000008:administration & dosage; D009206:Myocardium / Q000166:cytology; D009926:Organ Preservation; D011189:Potassium Chloride / Q000008:administration & dosage; D012965:Sodium Chloride / Q000008:administration & dosage
chemical_list: D002314:Cardioplegic Solutions; C059940:Roe cardioplegic solution; D015636:Magnesium Chloride; D012965:Sodium Chloride; D011189:Potassium Chloride; D005947:Glucose