Comparative studies on the expression of gastrointestinal-cancer-associated antigen, PA8-15, CA19-9 and the blood-group antigens in non-malignant and malignant human pancreatic tissues.
The expression of PA8-15 antigen and the blood-group-related antigens A, B, O, Le(a), Le(b), Le(x), and Le(y), as well as CA19-9, were examined in the normal pancreas and in specimens from benign and malignant pancreatic tissue by the avidin-biotin-immunoperoxidase technique. A correlation was found between the expression of PA8-15, Le(a), and CA19-9 in some cases. However, in the cancer tissues in which neither Le(a) nor CA19-9 could be demonstrated, strong expression of PA8-15 was observed. The reactivity of monoclonal antibody (mAb) PA8-15 with pancreatic cancer tissue was not inhibited by the preincubation of the sections with the mAb anti-Le(a) (CO514) and mAb CA19-9 (CO19-9) indicating that the epitope recognized by PA8-15 is different from that detected by the other two antibodies. Moreover, unlike Le(a) and CA19-9, PA8-15 was also expressed in cancer cells of patients of the Le(a-b-) type. The results suggest that mAb PA8-15 recognizes a sialylated molecule related to Le(a) but different from CA19-9, and seems to be an additional useful marker for pancreatic cancer.
Egami
H
H
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha.
Sakamoto
K
K
Yoshimura
R
R
Arai
M
M
Takiyama
Y
Y
Pour
P M
PM
D000230:Adenocarcinoma / Q000276:immunology*; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis*; D001789:Blood Group Antigens / Q000276:immunology*; D006801:Humans; D009439:Neuraminidase / Q000494:pharmacology; D010179:Pancreas / Q000276:immunology*; D010190:Pancreatic Neoplasms / Q000276:immunology*; D010195:Pancreatitis / Q000276:immunology*
D015295:Antigens, Tumor-Associated, Carbohydrate; D001789:Blood Group Antigens; D009439:Neuraminidase