[Reactivation of phosphorylated cholinesterase immobilized in a gelatin membrane].
The soluble and immobilized cholinesterases (acetyl cholinesterase of human blood erythrocytes (EC 3.1.1.7) and butyryl cholinesterase of equine blood serum, (EC 3.1.1.8] were inactivated by such irreversible inhibitors as diisopropyl fluorophosphate (DFP), O,O-dimethyl-O-(2,2)-dichlorovinyl) phosphate (DDVP), paraoxone, armine. The inactivated enzymes were reactivated under the effect of TMB-4 (1,1'-trimethylene-bis)-4-formyl-pyridine bromide (dioxime). The values of the reactivation rate constants proved to be equal both for the soluble and immobilized cholinesterases inactivated by the same irreversible inhibitor. The immobilized enzyme is simpler and more correct to study the reactivating action than the soluble one.
Kugusheva
L I
LI
Nikol'skaia
E B
EB
D000110:Acetylcholinesterase / Q000097:blood / Q000378:metabolism*; D000818:Animals; D002091:Butyrylcholinesterase / Q000097:blood / Q000378:metabolism*; D002800:Cholinesterase Inhibitors; D002801:Cholinesterase Reactivators; D002802:Cholinesterases / Q000378:metabolism*; D004800:Enzymes, Immobilized; D005780:Gelatin; D006736:Horses; D006801:Humans; D007700:Kinetics; D008567:Membranes, Artificial
D002800:Cholinesterase Inhibitors; D002801:Cholinesterase Reactivators; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D005780:Gelatin; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D002802:Cholinesterases
Reaktivatsiia fosforilirovannoĭ kholinésterazy, immobilizovannoĭ v zhelatinovoĭ membrane.