title: High mobility group proteins 1 and 2 bind preferentially to brominated poly(dG-dC).poly(dG-dC) in the Z-DNA conformation but not to other types of Z-DNA.
abstract: Three proteins from bull testis, previously thought to be Z-DNA-binding proteins but recently found to recognize brominated poly(dG-dC). poly(dG-dC) by criteria different from the Z-conformation, were partially sequenced. Of these, the 31 kDa protein was identified as a member of the high mobility group 2 protein family, and the 33 kDa protein as a member of the high mobility group 1 protein family. Both proteins had molecular weights approximately 30% higher than expected, indicating considerable posttranslational modification. In contrast, the 58 kDa protein remained unidentified for lack of any significant homology with known protein sequences.
authors.affiliation: Institut für Pharmakologie und biochemie, Universität Zürich-Irchel, Switzerland.
authors.forename: T
authors.initials: T
authors.lastname: Christen
authors.forename: M
authors.initials: M
authors.lastname: Bischoff
authors.forename: R
authors.initials: R
authors.lastname: Hobi
authors.forename: C C
authors.initials: CC
authors.lastname: Kuenzle
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001965:Bromides / Q000378:metabolism*; D002417:Cattle; D004247:DNA; D006609:High Mobility Group Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D011089:Polydeoxyribonucleotides / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D013737:Testis / Q000378:metabolism*
chemical_list: D001965:Bromides; D006609:High Mobility Group Proteins; D011089:Polydeoxyribonucleotides; C019530:poly(dC-dG); D004247:DNA