Two-dimensional polyacrylamide gel electrophoresis of human hair matrix proteins using isoelectric focusing electrophoresis as the first dimension.
S-carboxymethylated (SCM) matrix proteins from fifteen normal human scalp hair specimens were analyzed using two-dimensional polyacrylamide gel electrophoresis (PAGE) with usual isoelectric focusing electrophoresis as the first dimension and then with usual sodium dodecyl sulfate-PAGE as the second dimension. On the electrophoregrams obtained, one low-mobility smear of SCM proteins embedding one SCM protein band was always seen. Many higher-mobility SCM protein spots were also always seen. However, some variations were seen in these SCM protein compositions; in type I, there were eighteen SCM protein components and, in type II, seventeen components.
Dekio
S
S
Department of Dermatology, Shimane Medical University, Izumo, Japan.
Jidoi
J
J
D000328:Adult; D000596:Amino Acids / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006197:Hair / Q000032:analysis*; D006801:Humans; D007525:Isoelectric Focusing; D008840:Microfilament Proteins / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate
D000596:Amino Acids; D008840:Microfilament Proteins; D012967:Sodium Dodecyl Sulfate