[An immunohistochemical and in situ hybridization study of carbamyl phosphate synthetase I in altered liver cells during carcinogenesis].
In order to study the changes of phenotype and gene expression of carbamyl phosphate synthetase I (CPS 1) in preneoplastic altered liver cells in situ, serial cryostat sections of rat liver initiated by diethylnitrosamine (DENO) and promoted by phenobarbital (PB) were hybridized with 35S-GPS 1 cDNA probe by in situ hybridization, and immunohistochemical demonstration (PAP) of CPS 1 were made simultaneously. The results showed that abnormal expression of CPS 1 in the altered foci and nodules increased much more rapidly than that in normal ones, and consequently they might be more susceptable to develop into tumor.
Yang
Y P
YP
Department of Pathology, Faculty of Basic Medicine, Peking Union Medical College, Beijing.
D000818:Animals; D002222:Carbamoyl-Phosphate Synthase (Ammonia) / Q000235:genetics*; D004052:Diethylnitrosamine; D005260:Female; D015870:Gene Expression; D007150:Immunohistochemistry; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000473:pathology*; D008297:Male; D009693:Nucleic Acid Hybridization; D010634:Phenobarbital; D010641:Phenotype; D011230:Precancerous Conditions / Q000139:chemically induced / Q000473:pathology*; D051381:Rats; D011916:Rats, Inbred F344
D004052:Diethylnitrosamine; D002222:Carbamoyl-Phosphate Synthase (Ammonia); D010634:Phenobarbital