title: [Distribution and transcription of determinants homologous to the alpha-hemolysin (hlY) gene in the genomes of HLY- and HLY+ staphylococci].
abstract: The molecular organization of the alpha-hemolysin gene from Staphylococcus aureus strain O15 has been studied. Hybridization of the DNA from this strain with the [32P]-labeled cloned fragment containing alpha-hemolysin gene has shown the studied gene to be unique in the genome of the strain. Sequences homologous to the gene were not found to be dispersed along the genome of Staphylococcus aureus O15. The presence of alpha-hemolysin gene in some Staphylococcus epidermidis strains has been monitored. The strains 1413, 303 have been demonstrated to contain no gene for alpha-hemolysin. The hemolysin genes were found in the genomes of the strains 159, 169, 180 by DNA hybridization technique. In the genome of the strain 180 the long region including the right end of alpha-hemolysin gene is deleted. Hybridization with the net RNA of these strains shows the absence of transcription of intact as well as deleted alpha-hemolysin genes.
authors.forename: D K
authors.initials: DK
authors.lastname: Meshveliani
authors.forename: R Sh
authors.initials: RSh
authors.lastname: Adamiia
authors.forename: L I
authors.initials: LI
authors.lastname: Kvachadze
authors.forename: R P
authors.initials: RP
authors.lastname: Vashakidze
authors.forename: N A
authors.initials: NA
authors.lastname: Mamulashvili
mesh_terms: D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D006459:Hemolysin Factors / Q000235:genetics*; D006460:Hemolysin Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013212:Staphylococcus epidermidis / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D004269:DNA, Bacterial; D006460:Hemolysin Proteins
vernacular_title: Raspredelenie i transkriptsiia determinant, gomologichnykh genu alpha-gemolizina (hlY), v genomakh HLY- i HLY+ stafilokokkov.