[The physiologic significance of 2,3-diphosphoglycerate and changes in its erythrocyte levels in an experiment].
The regulatory effect of 2,3-DPG on oxygen transport and binding to hemoglobin was analyzed. Under conditions of substitution hemodilution by isooncotic 3.4% Rheodextran (Spofa), changes in the content of 2,3-DPG in arterial and venous blood were enzymatically determined over several days. Reference values of 2,3-DPG were obtained in the studied series of dogs (2.05 +/- 0.74 x 10(-6) mol.ml-1 in whole blood 4.69 +/- 1.52 x 10(-6) mol.g-1 of erythrocyte volume, and 13.39 +/- 2.82 x 10(-6) mol.ml-1 of hemoglobin). In anesthetized animals the content of 2,3-DPG in arterial blood was significantly higher (6.28 +/- 0.84 x 10(-6) mol.ml-1 of erythrocyte volume) than in venous blood (6.01 +/- 0.80 x 10(-6) mol.ml-1 of erythrocyte volume). At substitution hemodilution the 2,3-DPG content in erythrocytes of venous blood decreased from 5.46 +/- 0.67 to 4.97 +/- 1.31 x 10(-6) mol.ml-1 of erythrocyte volume. The subsequent increase to 6.04 +/- 0.71 x 10(-6) mol.ml-1 of erythrocyte volume was achieved by nonlinear increase over the following days, at persisting low hemoglobin content in blood.
Béder
I
I
Katedry fyziológie LFUK, Bratislava.
Orgonásová
M
M
Brozman
B
B
Horecký
J
J
Mataseje
A
A
D019794:2,3-Diphosphoglycerate; D000818:Animals; D001802:Blood Substitutes; D004163:Diphosphoglyceric Acids / Q000097:blood*; D004285:Dogs; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D006438:Hemodilution; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D008297:Male
D001802:Blood Substitutes; D004163:Diphosphoglyceric Acids; D006454:Hemoglobins; D019794:2,3-Diphosphoglycerate
Fyziologický význam 2,3-difosfoglycerátu a zmeny jeho obsahu v erytrocytoch pocas experimentu.