Glomerular basement membrane: evidence for collagenous domain of the alpha 3 and alpha 4 chains of collagen IV.
A collagenous component(s) of Mr = 60K was extracted from glomerular basement membrane with urea and was purified. Upon digestion, it yielded a collagenase-resistant fragment(s) of Mr = 23.5K. Both component and fragment showed immunochemical identity with the noncollagenous domains of the new alpha 3 & alpha 4 chains of collagen IV. The component is characterized by a collagenous domain of about 280 residues and a noncollagenous domain of about 250 residues. These findings further establish these new chains as distinct entities of collagen IV.
Fagg
W R
WR
Department of Biochemistry, University of Kansas Medical Center, Kansas City.
Timoneda
J
J
Schwartz
C E
CE
Langeveld
J P
JP
Noelken
M E
ME
Hudson
B G
BG
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001485:Basement Membrane / Q000032:analysis; D002417:Cattle; D003094:Collagen / Q000032:analysis*; D007678:Kidney Glomerulus / Q000032:analysis*; D046911:Macromolecular Substances; D011487:Protein Conformation
D000596:Amino Acids; D046911:Macromolecular Substances; D003094:Collagen