Hypoglycaemic activity of dioscoretine from tubers of Dioscorea dumetorum in normal and alloxan diabetic rabbits.
Dioscoretine isolated from the aqueous fraction of the methanol extract of Dioscorea dumetorum tubers when administered intra-peritoneally to normal and alloxan diabetic rabbits produces significant hypoglycaemic effects at a dose of 20 mg/kg. The fasting blood sugar in normoglycaemic rabbits was reduced from 112 mg/100 ml to 55 mg/100 ml after 4 hours. In alloxan diabetic rabbits, the blood sugar was lowered from 520 mg/100 ml to 286 mg/100 ml at the same time interval. The aqueous fraction of the methanol extract produced comparable effects at 100 mg/kg. The chloroform fraction of the same extract raised the fasting blood sugar of normal rabbits to 196 mg/100 ml after 6 h. The acute toxicity studies gave LD50 values of 1.4 g/kg for the aqueous fraction and 0.58 g/kg for dioscoretine when tested on mice. The hypoglycaemic effects were compared to those of tolbutamide.
Iwu
M M
MM
Phytotherapy Research Laboratory, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka.
Okunji
C O
CO
Ohiaeri
G O
GO
Akah
P
P
Corley
D
D
Tempesta
M S
MS
D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use / Q000633:toxicity; D000496:Alloxan; D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D007004:Hypoglycemic Agents / Q000302:isolation & purification / Q000627:therapeutic use*; D010946:Plants, Medicinal / Q000032:analysis; D011817:Rabbits
D000470:Alkaloids; D019086:Bridged Bicyclo Compounds, Heterocyclic; D007004:Hypoglycemic Agents; C064385:dioscoretine; D000496:Alloxan