title: Washout of the pig liver with Haemaccel after hypothermic preservation.
abstract: In hepatic preservation by simple perfusion and hypothermic storage, a portal and hepatic washout before revascularization would avoid receptor hyperkaliema. In this report we study the effectiveness of this washout with Haemaccel at room temperature. Large-White pigs were used and eight livers were perfused "in situ" via the portal wein with Hartmann's solution containing 10,000 IU of heparin at 4 degrees C, and afterwards, via portal and arterial routes with C2 solution at 4 degrees C. After a cold ischemia time of less than 31/2 hours a liver washout via the portal vein and hepatic artery with Haemaccel before portal revascularization was done. The high concentrations of glucose, K+, GOT, GPT and LDH in the effluents obtained during the washout are attributed to Haemaccel hyperosmolarity. A portal and arterial hepatic washout associated with free drainage of the first 50-100 ml of portal venous blood after hepatic portal revascularization through the infrahepatic inferior vena cava (IH-IVC), prevents hyperkaliemia from occurring after a portal and arterial revascularization in the orthotopic liver transplant (OLT) in pigs.
authors.affiliation: I Cátedra de Patología Quirúrgica, Hospital Universitario San Carlos, Universidad Complutense de Madrid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Arias
authors.forename: M A
authors.initials: MA
authors.lastname: Aller
authors.forename: L
authors.initials: L
authors.lastname: Lorente
authors.forename: J C
authors.initials: JC
authors.lastname: Rodriguez
authors.forename: X
authors.initials: X
authors.lastname: Fernández
authors.forename: D
authors.initials: D
authors.lastname: Brandau
authors.forename: H
authors.initials: H
authors.lastname: Durán
mesh_terms: D000818:Animals; D003080:Cold Temperature; D006982:Hypertonic Solutions / Q000008:administration & dosage; D007552:Isotonic Solutions / Q000008:administration & dosage; D008099:Liver / Q000098:blood supply*; D016031:Liver Transplantation; D009926:Organ Preservation / Q000379:methods*; D009994:Osmolar Concentration; D010477:Perfusion; D011097:Polygeline / Q000008:administration & dosage*; D011108:Polymers / Q000008:administration & dosage*; D000077325:Ringer's Lactate; D000077331:Ringer's Solution; D013552:Swine
chemical_list: C024102:Collins' solution; D006982:Hypertonic Solutions; D007552:Isotonic Solutions; D011108:Polymers; D000077325:Ringer's Lactate; D000077331:Ringer's Solution; D011097:Polygeline