[The study of nucleoside triphosphate-binding center of DNA-dependent RNA-polymerase of phage T7 using GTP analogs].
The NTP binding site of bacteriophage T7 DNA-dependent RNA polymerase was studied using GTP analogs. For four analogs the irreversible inhibition was demonstrated. The kinetic parameters for competitive (Ki) and irreversible (KI and k3) inhibition were determined. One of the analogs, 5'[2-hydroxy(4-iodoacetamido)benzoyl]guanosine, was shown to inactivate the enzyme rapidly due to the modification of SH-groups. Some suggestions on the structure of the RNA polymerase active site have been made.
Akbarov
A Kh
AKh
Tunitskaia
V L
VL
Baranova
L A
LA
Khropov
Iu V
IuV
Krasil'nikova
M M
MM
Kochetkov
S N
SN
D001665:Binding Sites; D001667:Binding, Competitive; D055598:Chemical Phenomena; D002621:Chemistry; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives* / Q000378:metabolism; D007202:Indicators and Reagents; D007700:Kinetics; D013604:T-Phages / Q000201:enzymology*
D007202:Indicators and Reagents; D006160:Guanosine Triphosphate; D012321:DNA-Directed RNA Polymerases
Issledovanie nukleozidtrifosfatsviazyvaiushchego tsentra DNK- zavisimoĭ RNK-polimerazy faga T7 s pomoshch'iu analogov GTP.