title: The relative roles of the glutathione redox cycle and catalase in the detoxification of H2O2 by cultured rabbit lens epithelial cells.
abstract: The relative roles of the glutathione redox cycle and catalase in the detoxification of H2O2 were investigated in cultured rabbit lens epithelial cells. Exposure of cells to H2O2 was carried out following inhibition of either of the two antioxidant systems. Two different procedures were used to expose the cells to extracellular H2O2, one in which a low, steady state level of 0.025 mM H2O2 was maintained in the culture medium with the use of glucose oxidase and the other in which H2O2 was added to the medium as a single pulse at levels ranging from 0.03 to 0.5 mM. When lens cells were treated with a low, steady state level of H2O2, the glutathione redox cycle was the primary means of defense against oxidative damage. Cells with fully active catalase but with inhibited glutathione reductase were not able to resist the cytotoxic effects of a 0.025 mM level of extracellular H2O2. Under these conditions the cells were nearly completely depleted of reduced glutathione within 15 min. The cellular damage observed after 1.5 hr of culture included loss of cell-to-cell contact, rounding up of the cells and formation of numerous blebs. In contrast, cells with completely inhibited catalase but with an unimpaired glutathione redox cycle suffered few damaging effects from a 3-hr exposure to 0.025 mM H2O2. When lens cells were pulsed with a single challenge of 0.5 mM H2O2, both the glutathione redox cycle and catalase were found to be essential for survival of the cells. While control cells were able to withstand the pulse of H2O2, cells with impaired activities of either the glutathione redox cycle or catalase were killed. Control cells treated with 0.5 mM H2O2 may have been protected from damage by the fact that the cellular level of GSH never dropped below 35% of normal. The cause of cell death following inhibition of catalase appeared to be related to an inability of the cells to remove peroxide from the culture medium, at a rapid rate, following the H2O2-pulse. Although cells with impaired glutathione reductase activity removed H2O2 from the medium at a rate comparable to that of control cells (due to uninhibited catalase activity), they did not survive the challenge.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Eye Research Institute, Oakland University, Rochester, MI 48309-4401.
authors.forename: F J
authors.initials: FJ
authors.lastname: Giblin
authors.forename: J R
authors.initials: JR
authors.lastname: Reddan
authors.forename: L
authors.initials: L
authors.lastname: Schrimscher
authors.forename: D C
authors.initials: DC
authors.lastname: Dziedzic
authors.forename: V N
authors.initials: VN
authors.lastname: Reddy
mesh_terms: D000818:Animals; D002374:Catalase / Q000502:physiology*; D002478:Cells, Cultured; D004848:Epithelium / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000493:pharmacokinetics*; D008658:Inactivation, Metabolic; D007908:Lens, Crystalline / Q000378:metabolism*; D010084:Oxidation-Reduction; D011817:Rabbits
chemical_list: D006861:Hydrogen Peroxide; D002374:Catalase; D005978:Glutathione