title: Prolactin stimulates milk protein promoter in CHO cells cotransfected with prolactin receptor cDNA.
abstract: A functional biological system was developed by cotransfecting mammalian cell lines with the cDNA of the prolactin receptor (PRL-R) and a fusion gene containing the promoter of the milk protein, ovine beta-lactoglobulin linked to the coding sequence of the chloramphenicol acetyltransferase (CAT) gene. Surprisingly, this system is effective even if a non-mammary cell line is used, since Chinese hamster ovary (CHO) cells transfected both transiently and stably with PRL-R cDNA respond to PRL, as observed by stimulation of the reporter gene. This newly developed system should help precisely define the functional domains of both the PRL-R molecule and of the regulatory elements of a PRL target gene.
authors.affiliation: Unité d'Endocrinologie Moléculaire, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
authors.forename: L
authors.initials: L
authors.lastname: Lesueur
authors.forename: M
authors.initials: M
authors.lastname: Edery
authors.forename: J
authors.initials: J
authors.lastname: Paly
authors.forename: J
authors.initials: J
authors.lastname: Clark
authors.forename: P A
authors.initials: PA
authors.lastname: Kelly
authors.forename: J
authors.initials: J
authors.lastname: Djiane
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004247:DNA / Q000235:genetics*; D015870:Gene Expression; D007782:Lactoglobulins / Q000235:genetics*; D011388:Prolactin / Q000494:pharmacology*; D011401:Promoter Regions, Genetic; D011981:Receptors, Prolactin / Q000235:genetics*; D014162:Transfection
chemical_list: D007782:Lactoglobulins; D011981:Receptors, Prolactin; D011388:Prolactin; D004247:DNA