Hypotonic treatment leads to chromosomal aberrations but not to sister-chromatid exchanges in human lymphocytes.
Human peripheral lymphocytes were isolated from whole blood and exposed to culture medium of reduced osmolality. This hypotonic treatment led to a significant increase in the frequencies of chromosomal aberrations when the osmolality was reduced to 60 mOsm/kg H2O and below. Maximum damage occurred when the hypotonic treatment was done 27 or 30 h after starting the cultures. We also looked for the induction of sister-chromatid exchanges (SCE) by hypotonic culture conditions, but the SCE frequencies were not influenced.
Kalweit
S
S
Freie Universität Berlin, Institut für Genetik, Germany.
Nowak
C
C
Obe
G
G
D002453:Cell Cycle; D002869:Chromosome Aberrations / Q000235:genetics*; D003470:Culture Media; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008214:Lymphocytes / Q000648:ultrastructure*; D008940:Mitotic Index; D009994:Osmolar Concentration; D012854:Sister Chromatid Exchange / Q000235:genetics*
D003470:Culture Media