[Peripheral metabolism of Pseudomonal putida transconjugants degrading chloro- and methylaromatic compounds].
Peripheral metabolism was studied in the Pseudomonas putida 37cc transconjugant. In the strain grown on benzoate, pyrocatechase (PC) I with a low activity to chlorocatechols was induced, whereas PCII actively decomposing chlorocatechols was induced during its growth on 3-chlorobenzoic acid. The P. putida 37cc transconjugant grown on alpha-methylstyrene (MS) exerted the activity of both metapyrocatechase (MPC) and PC, whereas in the parent strain P. putida R-1 only MPC was involved in the degradation of alpha-MS. The substrate specificity of the enzymes involved in the ring cleavage by P. putida 37cc was compared to show that, apparently, MPC of the transconjugant was similar to this enzyme in the strain R-1 while PC decomposing chlorocatechols was similar to PC of the P. putida 87 donor. The regulation of the enzymes mediating the ring cleavage was studied in the parent strains and transconjugants.
Mal'tseva
O V
OV
Golovleva
L A
LA
D001673:Biodegradation, Environmental; D002723:Chlorobenzoates / Q000493:pharmacokinetics* / Q000494:pharmacology; D003227:Conjugation, Genetic; D004790:Enzyme Induction; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D013343:Styrenes / Q000493:pharmacokinetics* / Q000494:pharmacology; D015262:Xenobiotics / Q000493:pharmacokinetics*
D002723:Chlorobenzoates; D013343:Styrenes; D015262:Xenobiotics; C036427:3-chlorobenzoic acid; C027142:vinyltoluene
Periferiĭnyĭ metabolizm transkon''iugantov Pseudomonas putida, razlagaiushchikh khlor- i metilaromaticheskie soedineniia.