Characterization of immune complexes by isoelectric focusing in agarose gels.
A method is described for the characterization of immune complexes on thin-layer agarose isoelectric focusing (IEF) gels. This method involves dissociating immune complexes and then maintaining this dissociation during IEF in agarose gels containing 9 M urea. After IEF, the immune complex components can be quantitatively transferred to nitrocellulose in less than 15 min, and a variety of immunostaining procedures can be used to probe these blotted components. No loss of biological activity was detected in any of the blotted components.
Hoffman
W L
WL
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas.
Kelly
P J
PJ
Larrumbide
M
M
D000818:Animals; D000936:Antigen-Antibody Complex / Q000032:analysis*; D006801:Humans; D015151:Immunoblotting; D007525:Isoelectric Focusing / Q000379:methods*; D011817:Rabbits; D014508:Urea
D000936:Antigen-Antibody Complex; D014508:Urea