Factors influencing the formation of methemoglobin in desferrioxamine loaded erythrocytes.
Desferrioxamine (DFO) in therapeutically useful amounts was incorporated into red blood cells (RBC) by means of a dialysis method. Entrapped DFO interacted with hemoglobin which was indicated by the formation of methemoglobin. This process was influenced to a great extent by the duration and temperature of the incubation steps. In contrast to added methylene blue the co-entrapment of ascorbic acid proved to be a very effective measure for protecting hemoglobin from oxidation and maintaining intracellular 2,3-diphosphoglycerate (2,3-DPG). Hypernormal values of 2,3-DPG could be obtained by the incubation in solutions containing phosphate, inosine, and pyruvate. Loaded RBC prepared in such a way should be characterized by a nearly normal oxygen-transport function and survival in vivo.
Hellwig
U
U
Blood Transfusion Centre, Berlin, DDR.
D019794:2,3-Diphosphoglycerate; D003676:Deferoxamine / Q000494:pharmacology*; D004163:Diphosphoglyceric Acids / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006461:Hemolysis; D006801:Humans; D007700:Kinetics; D008706:Methemoglobin / Q000378:metabolism*; D008751:Methylene Blue / Q000494:pharmacology
D004163:Diphosphoglyceric Acids; D019794:2,3-Diphosphoglycerate; D008706:Methemoglobin; D003676:Deferoxamine; D008751:Methylene Blue