title: Design of DNA-binding peptides based on the leucine zipper motif.
abstract: A class of transcriptional regulator proteins bind to DNA at dyad-symmetric sites through a motif consisting of (i) a "leucine zipper" sequence that associates into noncovalent, parallel, alpha-helical dimers and (ii) a covalently connected basic region necessary for binding DNA. The basic regions are predicted to be disordered in the absence of DNA and to form alpha helices when bound to DNA. These helices bind in the major groove forming multiple hydrogen-bonded and van der Waals contacts with the nucleotide bases. To test this model, two peptides were designed that were identical to natural leucine zipper proteins only at positions hypothesized to be critical for dimerization and DNA recognition. The peptides form dimers that bind specifically to DNA with their basic regions in alpha-helical conformations.
authors.affiliation: Central Research and Development Department, E.I. du Pont de Nemours & Co., Wilmington, DE 19880-0328.
authors.forename: K T
authors.initials: KT
authors.lastname: O'Neil
authors.forename: R H
authors.initials: RH
authors.lastname: Hoess
authors.forename: W F
authors.initials: WF
authors.lastname: DeGrado
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002942:Circular Dichroism; D003198:Computer Simulation; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006860:Hydrogen Bonding; D007930:Leucine; D046911:Macromolecular Substances; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation
chemical_list: D004268:DNA-Binding Proteins; D046911:Macromolecular Substances; D004247:DNA; D007930:Leucine