Osmotic regulation of beta(1-2) glucan synthesis in members of the family Rhizobiaceae.
High osmolarity in the culture medium of growing Agrobacterium tumefaciens strongly inhibited the accumulation of cellular beta(1-2) glucan. However, the enzymatic system required for the synthesis of this polysaccharide from UDP-glucose was not repressed by high osmolarity. Mutants of A. tumefaciens and Rhizobium meliloti affected in beta(1-2) glucan synthesis were unable to grow normally in low-osmolarity media.
Zorreguieta
A
A
Instituto de Investigaciones Bioquimicas, Fundación Campomar, CONICET, Buenos Aires, Argentina.
Cavaignac
S
S
Geremia
R A
RA
Ugalde
R A
RA
D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D005936:Glucans / Q000096:biosynthesis* / Q000302:isolation & purification; D007700:Kinetics; D008565:Membrane Proteins / Q000302:isolation & purification; D008970:Molecular Weight; D009994:Osmolar Concentration; D012230:Rhizobiaceae / Q000254:growth & development / Q000378:metabolism*; D012231:Rhizobium / Q000378:metabolism; D014532:Uridine Diphosphate Glucose / Q000378:metabolism; D047071:beta-Glucans
D003470:Culture Media; D005936:Glucans; D008565:Membrane Proteins; D047071:beta-Glucans; C033744:beta-1,2-glucan; D014532:Uridine Diphosphate Glucose