Skeletal muscle extracts promote the survival of neurofilament-positive mammalian sensory neurons.
Cultured neurons of the mammalian dorsal root ganglia (DRG) can be divided, as in intact ganglia, into two classes: 'large light' (neurofilament-positive) and 'small dark' (neurofilament-negative) neurons. While 'small dark' neuron survival depends on NGF during ontogenesis, little is known about the neurotrophic factor requirement of the 'large light' sub-population. This study demonstrates that the in vitro survival of neonatal mammalian neurofilament-positive DRG neurons requires the presence of a neurotrophic factor present in skeletal muscle extract.
Valmier
J
J
Laboratoire de Médecine Expérimentale, INSERM U249, Institut de Biologie, Montpellier, France.
Tafti
M
M
Baldy-Moulinier
M
M
D000818:Animals; D000831:Animals, Newborn; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000648:ultrastructure*; D005727:Ganglia, Spinal / Q000166:cytology; D007382:Intermediate Filaments / Q000648:ultrastructure*; D051379:Mice; D009132:Muscles / Q000502:physiology*; D009414:Nerve Growth Factors / Q000494:pharmacology; D009475:Neurons, Afferent / Q000166:cytology* / Q000187:drug effects; D014020:Tissue Extracts / Q000494:pharmacology*
D009414:Nerve Growth Factors; D014020:Tissue Extracts