title: Induction of an ATP-polymerizing enzyme in TMV-infected tobacco and its homology to the human 2'-5' A synthetase.
abstract: Several reports have indicated that tobacco carries an enzyme (APE) that, in the presence of poly (rI):(rC), polymerizes ATP to oligoadenylates. This paper demonstrates that the tobacco APE system comprises several proteins (estimated sizes: 32, 42, 67, and 84 +/- 10% kD). Only one of these proteins (the "67-kD" form) binds to poly (rI):(rC). This APE form has been purified by affinity chromatography on a synthetic ds-RNA column. Four tobacco proteins, including the purified one, crossreact with antibodies against the human enzyme, 2'-5' A synthetase. The ATP-binding capacity of some of these proteins has also been demonstrated. The amount of plant oligoadenylates obtained by polymerizing ATP with the purified APE form allows, for the first time, their direct analysis by TLC. The TLC analysis indicated that the oligomer produced by APE is not identical to the 2'-5' oligoadenylate. The appearance of the 2'-5' A-related proteins correlates with the build up of TMV infection, and the pattern of their stimulation and turnover was established. Nucleic acid hybridization indicates homology of tobacco DNA and RNA sequences with cloned cDNA of the human 2'-5' A synthetase gene. The stimulation in tobacco, upon TMV infection, of mRNA species homologous to the above human cDNA has been demonstrated. The analogy between the plant and the human system is discussed.
authors.affiliation: Virus Laboratory, Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel.
authors.forename: N
authors.initials: N
authors.lastname: Sher
authors.forename: O
authors.initials: O
authors.lastname: Edelbaum
authors.forename: Z
authors.initials: Z
authors.lastname: Barak
authors.forename: G
authors.initials: G
authors.lastname: Grafi
authors.forename: Y
authors.initials: Y
authors.lastname: Stram
authors.forename: J
authors.initials: J
authors.lastname: Raber
authors.forename: I
authors.initials: I
authors.lastname: Sela
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D002855:Chromatography, Thin Layer; D003429:Cross Reactions; D004279:DNA, Viral / Q000276:immunology*; D004790:Enzyme Induction; D006801:Humans; D008025:Ligases / Q000235:genetics* / Q000276:immunology; D009843:Oligoribonucleotides / Q000378:metabolism; D010944:Plants / Q000382:microbiology*; D011500:Protein Synthesis Inhibitors / Q000378:metabolism; D012333:RNA, Messenger / Q000276:immunology*; D012689:Sequence Homology, Nucleic Acid; D014027:Tobacco Mosaic Virus / Q000201:enzymology / Q000235:genetics*; D014777:Virus Diseases / Q000235:genetics / Q000276:immunology
chemical_list: D000227:Adenine Nucleotides; D004279:DNA, Viral; D009843:Oligoribonucleotides; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; C023505:2',5'-oligoadenylate; D000255:Adenosine Triphosphate; D008025:Ligases