Biochemical and physical analysis of subcellular membranes in rat parotid gland enlarged by chronic isoproterenol administration.
1. Chronic administration of isoproterenol caused similar alterations of membrane lipid profile in at least two rat parotid subcellular fractions, secretory granular and microsomal. 2. Typical changes in phospholipid classes and fatty-acyl chain groups were an increase of phosphatidylcholine and a decrease of sphingomyelin, and an increase of octadecadienoyl chain and a decrease of eicosatetraenoyl chain, respectively. 3. Electron spin resonance study showed that the isoproterenol-treatment also affected a membrane physical property, which may be through these compositional changes in membrane constituents.
Mizuno
M
M
Department of Oral Biochemistry, Asahi University School of Dentistry, Gifu, Japan.
Yashiro
K
K
Kameyama
Y
Y
Hayashi
S
S
Sakashita
Y
Y
Yokota
Y
Y
D000818:Animals; D003594:Cytoplasmic Granules / Q000187:drug effects / Q000378:metabolism; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism; D007545:Isoproterenol / Q000633:toxicity*; D008051:Lipid Bilayers / Q000378:metabolism; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D008563:Membrane Lipids / Q000378:metabolism; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D010306:Parotid Gland / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D008051:Lipid Bilayers; D008563:Membrane Lipids; D010743:Phospholipids; D007545:Isoproterenol