An electrophysiological study of 9-amino-1,2,3,4-tetrahydroacridine (THA) on sino-atrial node preparations of rabbits.
1. The electrophysiological effect of 9-amino-1,2,3,4-tetrahydroacridine (THA) on the rabbit sino-atrial node was studied using double-microelectrode voltage clamp methods. 2. THA (above 10 microM) caused statistically significant decreases in the maximum rate of rise, the action potential amplitude, the rate of diastolic depolarization, and increases in the spontaneous cycle length, the action potential duration at 50% repolarization. 3. On the current systems, THA obviously depressed the time-dependent outward K+ current. The compound also decreased the slow inward Ca2+ current and the hyperpolarization-activated inward current. 4. These findings indicate that THA exerts an inhibitory action on the automaticity of sino-atrial node via effects on both outward and inward current systems.
Kotake
H
H
1st Department of Internal Medicine, Tottori University School of Medicine, Yonago, Japan.
Noguchi
N
N
Doi
T
T
Matsuoka
S
S
Hisatome
I
I
Hasegawa
J
J
Mashiba
H
H
D000200:Action Potentials / Q000187:drug effects; D000609:Aminoacridines / Q000494:pharmacology*; D000818:Animals; D004594:Electrophysiology; D005260:Female; D006339:Heart Rate / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D008839:Microelectrodes; D015221:Potassium Channels / Q000187:drug effects; D011817:Rabbits; D012849:Sinoatrial Node / Q000187:drug effects*; D013619:Tacrine / Q000494:pharmacology*
D000609:Aminoacridines; D015221:Potassium Channels; D013619:Tacrine