A ubiquitous CCAAT factor is required for efficient in vitro transcription from the mouse albumin promoter.
Among the various factors binding to DNA elements within the mouse albumin promoter, NF-Y is the only one present at identical concentrations in the nuclei of all examined tissues. NF-Y binds to albumin promoter element C, which contains the sequence CCAAT. To determine whether this factor augments in vitro transcription from the albumin promoter, an extensive point-mutation analysis was performed within the promoter element C. In liver extracts, six out of the ten mutations result in a strong inhibition of NF-Y binding and in a concomitant decrease in promoter activity. Two mutations that increase the affinity of the C-element for NF-Y also augment the transcription efficiency from the albumin promoter. A similarly strong correlation of NF-Y-binding with transcription efficiency has also been observed in spleen nuclear extracts. The liver-enriched CCAAT and enhancer binding factor C/EBP also recognizes the C element. In contrast to NF-Y, no correlation between the affinity of mutant C-elements for C/EBP and transcriptional activity could be observed in liver nuclear extracts.
Wuarin
J
J
Department of Molecular Biology, University of Geneva, Switzerland.
Mueller
C
C
Schibler
U
U
D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D005786:Gene Expression Regulation; D008099:Liver / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D011401:Promoter Regions, Genetic; D012709:Serum Albumin / Q000235:genetics*; D013154:Spleen / Q000378:metabolism; D013698:Templates, Genetic; D014158:Transcription, Genetic
D015345:Oligonucleotide Probes; D012709:Serum Albumin