Isolation and characterization of the 32.5 kDa protein from the venom of an endoparasitic wasp.
The major venom proteins from the endoparasitic wasp were analyzed for distribution in the venom gland. A 32.5 kDa protein was purified from the venom gland of the Chelonus near curvimaculatus wasp. The protein accounts for about 25% of the total protein content of the venom and each gland contains 3-6 pmol of this component. The protein is acidic in nature and anion-exchange chromatography facilitated the purification of the protein to apparent homogeneity. On testing the purified protein by in vivo bioassay, it was found to elicit an effect comparable with the complete venom. The protein does not appear to have any disulfide bonds of major structural importance exposed under SDS-denaturing conditions. Products of chemical partial digest of the purified protein at the methionyl residues by cyanogen bromide were analyzed by SDS-PAGE. The 27.6 kDa fragment retained an epitope to an antibody raised against total Chelonus venom proteins, whereas no epitopes were detected for 4.9 and 0.6 kDa fragments.
Taylor
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Department of Entomology, University of Kentucky, Lexington 40546.
Jones
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D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001514:Bee Venoms / Q000302:isolation & purification*; D003488:Cyanogen Bromide; D004591:Electrophoresis, Polyacrylamide Gel; D006927:Hymenoptera / Q000032:analysis*; D015151:Immunoblotting; D007525:Isoelectric Focusing; D008623:Mercaptoethanol; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010449:Peptide Mapping; D014688:Venoms / Q000032:analysis*; D014862:Wasp Venoms / Q000302:isolation & purification*; D014863:Wasps / Q000032:analysis*
D000596:Amino Acids; D001514:Bee Venoms; D014688:Venoms; D014862:Wasp Venoms; D008623:Mercaptoethanol; D003488:Cyanogen Bromide