title: In vitro transformation induces abnormal protein thiol levels in rat liver cells.
abstract: Cells from an established line of normal rat hepatocytes (IAR 20) were irradiated with 3 Gy gamma-radiation from a cobalt source to generate transformed clones. Cells from four transformed colonies were compared with the parent cell line by flow cytometry following staining with ortho-phthalaldehyde and propidium iodide to quantitate protein thiols and DNA respectively. Transformed cells exhibited an increased variability in cellular protein thiols which was most evident in G1 and S phase. The altered pattern of macromolecular thiol distribution implies early changes in redox state and/or modification of the amounts or types of sulphur-containing proteins in transformed cells.
authors.affiliation: Department of Chemical Pathology, University College and Middlesex School of Medicine, London, UK.
authors.forename: P
authors.initials: P
authors.lastname: Principe
authors.forename: G D
authors.initials: GD
authors.lastname: Wilson
authors.forename: P A
authors.initials: PA
authors.lastname: Riley
authors.forename: T F
authors.initials: TF
authors.lastname: Slater
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D005434:Flow Cytometry; D005453:Fluorescence; D007399:Interphase; D008099:Liver / Q000032:analysis / Q000528:radiation effects*; D011506:Proteins / Q000032:analysis*; D051381:Rats; D013438:Sulfhydryl Compounds / Q000032:analysis*
chemical_list: D011506:Proteins; D013438:Sulfhydryl Compounds