title: Saccharopolyspora hirsuta strain 367 releases JHJ-1, a bacteriophage capable of propagation on old mycelium.
abstract: Bacteriophage JHJ-1 was isolated from Saccharopolyspora hirsuta strain 367 NRRL 12045 as an endogenous but virulent phage. The plaque size was not self-limiting, since a few p.f.u. could completely lyse a lawn. Electron microscopy showed that this phage belonged to group B of Bradley's morphological classification. The JHJ-1 genome is a linear DNA molecule of 41.1 kbp with cohesive ends and a G + C content of 68.8-70.0 mol%. The DNA cleavage map was established for 12 restriction endonucleases. The host range is apparently very narrow, being limited to two strains of S. hirsuta (NRRL 12045 and NRRL B-5792). However, JHJ-1 did not lytically infect S. hirsuta strain 367 UC 8106. Phage JHJ-1 was shown, by Southern blot analysis, to lysogenize both S. hirsuta NRRL 12045 and UC 8106. It thus appears to behave as a virulent mutant of a temperate phage on one, but not on the other, JHJ-1 lysogen.
authors.affiliation: Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Québec, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Haket
authors.forename: D
authors.initials: D
authors.lastname: Desmarais
authors.forename: K
authors.initials: K
authors.lastname: Mehindate
authors.forename: C V
authors.initials: CV
authors.lastname: Déry
mesh_terms: D000190:Actinomyces / Q000254:growth & development* / Q000648:ultrastructure; D001435:Bacteriophages / Q000235:genetics / Q000254:growth & development* / Q000648:ultrastructure; D001482:Base Composition; D004279:DNA, Viral / Q000032:analysis; D006790:Host-Parasite Interactions; D008242:Lysogeny; D009154:Mutation; D010641:Phenotype; D015183:Restriction Mapping; D010948:Viral Plaque Assay
chemical_list: D004279:DNA, Viral