DNA adducts in human environmentally exposed to aromatic compounds in an industrial area of Poland.
The effect of environmental pollution on DNA adducts in humans was analysed in a highly industrialized area of Poland. Coded samples of white blood cell DNA were analysed by 32P-postlabelling and immunoassay from three populations: coke workers, exposed occupationally to high levels of polycyclic aromatic hydrocarbons (PAHs); residents of the towns around cokeries (local controls); and residents from rural Poland (countryside controls). Local controls exhibited adduct levels and patterns similar to those of coke workers, while the levels in rural controls were 2-3 times lower. The results, based on coded samples and two different assays, suggest that environmental pollution is likely to contribute to the adduct levels in local controls. Furthermore, the results show that the levels of aromatic adducts in white blood cell DNA do not linearily relate to ambient air levels of PAHs but other sources such as food may be important contributors.
Hemminki
K
K
Institute of Occupational Health, Helsinki, Finland.
Grzybowska
E
E
Chorazy
M
M
Twardowska-Saucha
K
K
Sroczynski
J W
JW
Putman
K L
KL
Randerath
K
K
Phillips
D H
DH
Hewer
A
A
Santella
R M
RM
D000393:Air Pollutants; D000395:Air Pollutants, Occupational; D003031:Coal; D003077:Coke; D004247:DNA / Q000097:blood / Q000378:metabolism*; D006801:Humans; D007962:Leukocytes / Q000032:analysis*; D011044:Poland; D011083:Polycyclic Compounds / Q000097:blood / Q000378:metabolism*
D000393:Air Pollutants; D000395:Air Pollutants, Occupational; D003031:Coal; D003077:Coke; D011083:Polycyclic Compounds; D004247:DNA