A radioimmunoassay for antibodies to the Ro/SSA particle.
A radioimmunoassay (RIA) for antibodies to the Ro/SSA particle is described using Iodogen to radiolabel the antigenic protein moiety of Ro/SSA with 125I. RIA methods utilizing either 33% saturated ammonium sulfate (NH4)2SO4 or 3.5% polyethylene glycol to separate bound from free antigen are comparable. Either method is similar to ELISA in sensitivity and specificity. This RIA provides a fluid phase assay for the Ro/SSA-anti-Ro/SSA system not previously available.
Yoshida
S
S
Department of Medicine, Oklahoma University College of Medicine, Oklahoma Health Sciences Center, Oklahoma City.
Reichlin
M
M
D001323:Autoantibodies / Q000032:analysis*; D001324:Autoantigens / Q000276:immunology*; D001667:Binding, Competitive; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D008970:Molecular Weight; D020733:RNA, Small Cytoplasmic; D011863:Radioimmunoassay / Q000379:methods; D015531:Radioimmunoprecipitation Assay; D012261:Ribonucleoproteins; D014508:Urea / Q000031:analogs & derivatives; D000099207:SS-A Antigen
D001323:Autoantibodies; D001324:Autoantigens; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D014508:Urea; D000099207:SS-A Antigen; C116006:RO60 protein, human; C012385:1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenylglycoluril