title: Seminal fluid from men with agenesis of the Wolffian ducts: zinc-binding properties and effects on sperm chromatin stability.
abstract: Zinc-binding properties were studied in 'prostatic fluid', i.e. in seminal plasma from patients with agenesis of the Wolffian ducts, and in split-ejaculate fractions dominated by seminal vesicular fluid. The effect of seminal fluid, with different zinc-binding properties, on the stability of zinc-dependent sperm chromatin was assessed by exposing sperm to 1% sodium dodecyl sulphate (SDS) for 60 min. Citrate was the only zinc ligand in 'prostatic fluid', as revealed by gel chromatography. Zinc in this fluid enhanced the stability of sperm chromatin. In contrast, the stability of sperm chromatin was decreased in seminal plasma dominated by vesicular fluid. These results are in accordance with the concept that prostatic fluid ensures the appropriate zinc content and stability of sperm chromatin, whereas abundance of vesicular fluid may jeopardize chromatin stability by reducing chromatin zinc content.
authors.affiliation: Department of Clinical Chemistry, Karolinska Hospital, Stockholm, Sweden.
authors.forename: U
authors.initials: U
authors.lastname: Kvist
authors.forename: S
authors.initials: S
authors.lastname: Kjellberg
authors.forename: L
authors.initials: L
authors.lastname: Björndahl
authors.forename: J C
authors.initials: JC
authors.lastname: Soufir
authors.forename: S
authors.initials: S
authors.lastname: Arver
mesh_terms: D002843:Chromatin / Q000032:analysis / Q000187:drug effects*; D002850:Chromatography, Gel; D002951:Citrates / Q000032:analysis; D019343:Citric Acid; D006801:Humans; D008297:Male; D011467:Prostate / Q000378:metabolism*; D011485:Protein Binding; D012661:Semen / Q000032:analysis / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors; D014928:Wolffian Ducts / Q000002:abnormalities*; D015032:Zinc / Q000032:analysis / Q000378:metabolism*
chemical_list: D002843:Chromatin; D002951:Citrates; D019343:Citric Acid; D012967:Sodium Dodecyl Sulfate; D015032:Zinc