title: Optimization of multidimensional high-performance liquid chromatography for the determination of drugs in plasma by direct injection, micellar cleanup and photodiode array detection.
abstract: Improvements in a multidimensional liquid chromatography system for the direct determination of drug substances in blood plasma are reported. The system employs an on-line micellar chromatographic cleanup followed by a reversed-phase analytical separation. The limit of detection of propranolol is improved by a factor 10 compared to previously reported work. The technique is applied towards the determination of a multicomponent mixture of tricyclic anti-depressants in blood plasma. A protocol for optimization is described.
authors.affiliation: Applied Biosystems, Inc., Ramsey, NJ 07446.
authors.forename: J V
authors.initials: JV
authors.lastname: Posluszny
authors.forename: R
authors.initials: R
authors.lastname: Weinberger
authors.forename: E
authors.initials: E
authors.lastname: Woolf
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D004364:Pharmaceutical Preparations / Q000032:analysis*; D010949:Plasma / Q000032:analysis*; D011433:Propranolol / Q000097:blood
chemical_list: D004364:Pharmaceutical Preparations; D011433:Propranolol