The gene for Klebsiella bacteriophage K11 RNA polymerase: sequence and comparison with the homologous genes of phages T7, T3, and SP6.
We determined the nucleotide sequence of gene 1 of Klebsiella phage K11, which is a member of the T7 group of phages. The largest open reading frame corresponds to a polypeptide with 906 amino acids and a molecular weight of 100,383 daltons. The deduced amino acid sequence of this polypeptide shows 71% homology to the T7 RNA polymerase (the product of T7 gene 1), 72% homology to the T3 RNA polymerase and 27% homology to the SP6 RNA polymerase. Divergent evolution was clearly most pronounced in the amino-terminal portion.
Dietz
A
A
Institut für Biologie III der Universität Freiburg, Federal Republic of Germany.
Weisser
H J
HJ
Kössel
H
H
Hausmann
R
R
D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D005814:Genes, Viral; D007709:Klebsiella; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D013604:T-Phages / Q000201:enzymology / Q000235:genetics*
D012321:DNA-Directed RNA Polymerases