Isolation and partial characterization of a human amelogenin from a single fetal dentition using HPLC techniques.
A strategy based on high pressure liquid chromatography (HPLC) techniques for the isolation or a principal amelogenin molecule from a single human dentition is described. A partial sequence (33 residues) for this 24 kDa amelogenin is presented and related to earlier studies of human 5 kDa tyrosine-rich amelogenin polypeptides (TRAPs). A failure to identify amino acid residue #25 (tryptophan in other amelogenins) suggests that this 24 kDa amelogenin is the progenitor of the human TRAP-2 molecule and provides further support for the possibility of several human amelogenin gene products, generated by splice-junction selection, from the single amelogenin gene in the same individual. Alternatively, multiple amelogenins may arise by expression of both the AMELX and AMELY loci.
Fincham
A G
AG
Department of Basic Sciences (Biochemistry), University of Southern California, Los Angeles 90033.
Hu
Y Y
YY
Lau
E
E
Pavlova
Z
Z
Slavkin
H C
HC
Snead
M L
ML
D053523:Amelogenin; D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D003746:Dental Enamel Proteins / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification*; D003817:Dentition; D004591:Electrophoresis, Polyacrylamide Gel; D005333:Fetus / Q000032:analysis; D006801:Humans; D008969:Molecular Sequence Data
C507199:AMELX protein, human; C507202:AMELY protein, human; D053523:Amelogenin; D003746:Dental Enamel Proteins