Determination of lipid hydroperoxides in low density lipoprotein from human plasma using high performance liquid chromatography with chemiluminescence detection.
A high performance liquid chromatographic system with chemiluminescence detection (HPLC-CL) was used for determining phospholipid hydroperoxides in human plasma low density lipoprotein (LDL). This system involved separation of phospholipids from LDL-total lipids with normal phase silica gel HPLC and post-column detection of hydroperoxide-dependent chemiluminescence produced by luminol oxidation during the reaction of hydroperoxide with cytochrome c-haeme. By using HPLC-CL, we could detect phosphatidylcholine hydroperoxide (PCOOH) in human plasma LDL, and the LDL-PCOOH concentration was significantly higher in patients with atherosclerosis and hyperlipidemia than that of healthy volunteers. The LDL-PCOOH level was proportional to the plasma total cholesterol concentration.
Miyazawa
T
T
Department of Food Chemistry, Tohoku University, Sendai, Japan.
Fujimoto
K
K
Oikawa
S
S
D000293:Adolescent; D000328:Adult; D000368:Aged; D001161:Arteriosclerosis / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood; D008054:Lipid Peroxides / Q000032:analysis / Q000097:blood*; D008077:Lipoproteins, LDL / Q000032:analysis / Q000097:blood*; D008163:Luminescent Measurements; D008875:Middle Aged
D008054:Lipid Peroxides; D008077:Lipoproteins, LDL