title: Two-dimensional spectra of intact tissue: homonuclear Hartmann-Hahn spectroscopy provides increased sensitivity and information content as compared to COSY.
abstract: Hartmann-Hahn (HOHAHA) spectroscopy led to good definition of proton spin systems of metabolites such as lactate, (phospho) creatine, and carnosine from a 3-g sample of excised frog gastrocnemius muscle in a 17-min experiment. The sign of the HOHAHA cross-peak intensity appears to provide additional information about interactions between metabolites and their protein binding sites.
authors.affiliation: Department of Biooquímica i Biologia Molecular, Facultat de Ciències, Universitat Autònoma de Barcelona, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Alonso
authors.forename: C
authors.initials: C
authors.lastname: Arús
authors.forename: W M
authors.initials: WM
authors.lastname: Westler
authors.forename: J L
authors.initials: JL
authors.lastname: Markley
mesh_terms: D000818:Animals; D002336:Carnosine / Q000032:analysis; D007773:Lactates / Q000032:analysis; D019344:Lactic Acid; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009132:Muscles / Q000032:analysis*; D010725:Phosphocreatine / Q000032:analysis; D011894:Rana pipiens
chemical_list: D007773:Lactates; D010725:Phosphocreatine; D019344:Lactic Acid; D002336:Carnosine