title: Development and survival after transfer of cow embryos cultured from 1-2-cells to morulae or blastocysts in rabbit oviducts or in a simple medium with bovine oviduct epithelial cells.
abstract: This study compares development of bovine 1-2-cell embryos in bovine oviduct epithelial cell co-culture (Group EC) with a glucose- and serum-free simple medium (CZB), or after surgical transfer to ligated oviducts of rabbits (Group RO). Embryos were surgically collected from superovulated donor cows 40-48 h after the beginning of oestrus and randomly distributed between the two groups. Embryos were cultured or incubated for 5 days. In Exp. 1, embryo quality scores and total numbers of cells in the two groups were compared. In Exp. 2, pairs of similarly treated morulae were transferred to each of 10 or 12 recipients in the Groups RO and EC, respectively. Total cell counts per embryo in both groups averaged 52 (P greater than 0.05), and the in-vitro culture system was equivalent to the rabbit oviducts in promoting embryo development for all characteristics measured. Embryo survival, as determined by ultrasound between Days 39 and 43 after oestrus, in 13 ideal recipients was 57% for embryos in Group EC and 58% for embryos Group RO. None of the 9 less desirable recipients was pregnant for either group. These results establish that cattle zygotes can develop to morulae in culture with bovine oviduct epithelial cells in a simple medium and can produce normal pregnancy rates.
authors.affiliation: Department of Animal Science, Cornell University, Ithaca, New York 14853-4801.
authors.forename: J E
authors.initials: JE
authors.lastname: Ellington
authors.forename: P B
authors.initials: PB
authors.lastname: Farrell
authors.forename: M E
authors.initials: ME
authors.lastname: Simkin
authors.forename: R H
authors.initials: RH
authors.lastname: Foote
authors.forename: E E
authors.initials: EE
authors.lastname: Goldman
authors.forename: A B
authors.initials: AB
authors.lastname: McGrath
mesh_terms: D000818:Animals; D001755:Blastocyst; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D004624:Embryo Transfer / Q000379:methods*; D005314:Embryonic and Fetal Development; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology; D005187:Fallopian Tubes / Q000502:physiology*; D005260:Female; D005313:Fetal Death; D009028:Morula; D011247:Pregnancy; D011817:Rabbits
chemical_list: D003470:Culture Media