Chromium reduction in Pseudomonas putida.
Reduction of hexavalent chromium (chromate) to less-toxic trivalent chromium was studied by using cell suspensions and cell-free supernatant fluids from Pseudomonas putida PRS2000. Chromate reductase activity was associated with soluble protein and not with the membrane fraction. The crude enzyme activity was heat labile and showed a Km of 40 microM CrO4(2-). Neither sulfate nor nitrate affected chromate reduction either in vitro or with intact cells.
Ishibashi
Y
Y
Department of Microbiology and Immunology, University of Illinois, College of Medicine, Chicago 60680.
Cervantes
C
C
Silver
S
S
D002840:Chromates / Q000378:metabolism*; D007700:Kinetics; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D011549:Pseudomonas / Q000378:metabolism*
D002840:Chromates; D009243:NAD