Effect of patulin on the kinetic properties of the enzyme aldolase studied in rat liver.
The toxic nature of the secondary metabolite has been studied in rats. Changes in the concentration of a few key enzymes in carbohydrate metabolism have also been studied. In this, liver aldolase concentration was found to be significantly lowered. Since aldolase is one of the important bifunctional enzymes of glycolysis, it has been isolated and purified and studied on its kinetic properties were made. The kinetic studies did not show any significant variations in the properties of liver aldolase of normal and patulin treated animals. These results suggest that most probably, patulin toxicosis inhibits the biosynthesis of liver aldolase.
Sakthisekaran
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Department of Biochemistry, University of Madras, India.
Shanmugasundaram
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ER
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000249:Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D002850:Chromatography, Gel; D005260:Female; D005634:Fructose-Bisphosphate Aldolase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007422:Intestines / Q000201:enzymology; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008970:Molecular Weight; D010365:Patulin / Q000494:pharmacology / Q000633:toxicity*; D011714:Pyrans / Q000633:toxicity*; D051381:Rats; D013696:Temperature; D014508:Urea / Q000494:pharmacology
D011714:Pyrans; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D014508:Urea; D010365:Patulin; D005634:Fructose-Bisphosphate Aldolase