DNA probe culture confirmation assay for identification of thermophilic Campylobacter species.
We studied the ability of a new DNA probe-based assay system to correctly identify isolates of the thermophilic campylobacters Campylobacter jejuni, C. coli, and C. laridis grown in vitro. We examined 424 organisms, including 214 Campylobacter isolates and 210 other aerobic and anaerobic isolates. The probe assay, which uses a new homogeneous system in which all reactions take place within a single tube, demonstrated 100% accuracy, producing neither false-positive nor false-negative results. The assay does not, however, distinguish among C. jejuni, C. coli, and C. laridis.
Tenover
F C
FC
Seattle Veterans Affairs Medical Center, Washington 98108.
Carlson
L
L
Barbagallo
S
S
Nachamkin
I
I
D002167:Campylobacter / Q000254:growth & development / Q000302:isolation & purification*; D003470:Culture Media; D015342:DNA Probes; D006801:Humans; D008163:Luminescent Measurements
D003470:Culture Media; D015342:DNA Probes