title: Induction of antigen expression of follicular dendritic cells in a monoblastic cell line. A contribution to its cellular origin.
abstract: Experiments were carried out to elucidate the cellular origin of the dendritic reticulum cell (DRC). The monoblastic cell line THP-1, the histiocytic cell line U-937, and the mononuclear cell fraction from peripheral blood (PMC) were stimulated with supernatants from lectin-stimulated peripheral blood lymphocytes and from stimulated T- and B-cell lines. Differentiation towards DRC was assessed by immunocytochemical demonstration of the DRC-specific antigen Ki-M4. Supernatants from isolated peripheral T lymphocytes and from T- and B-cell lines were capable of stimulating THP-1 to Ki-M4 antigen expression, whereas U-937 and the PMC fraction remained negative for this antigen throughout the experiments. These results provide further evidence for a relationship of DRCs with the mononuclear-phagocytic cell system and hence for their bone marrow origin. Furthermore, the data suggest that soluble factors of T and/or B cells are involved in mediating the differentiation process of precursor cells to DRC.
authors.affiliation: Department of Pathology, University of Würzburg, F.R.G.
authors.forename: A
authors.initials: A
authors.lastname: Fliedner
authors.forename: M R
authors.initials: MR
authors.lastname: Parwaresch
authors.forename: A C
authors.initials: AC
authors.lastname: Feller
mesh_terms: D000954:Antigens, Surface / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D003713:Dendritic Cells / Q000276:immunology* / Q000473:pathology; D006801:Humans; D009000:Monocytes / Q000276:immunology* / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology; D014407:Tumor Cells, Cultured / Q000473:pathology
chemical_list: D000954:Antigens, Surface