title: Activities of (-)-carbovir and 3'-azido-3'-deoxythymidine against human immunodeficiency virus in vitro.
abstract: (-)-Carbovir, the minus optical isomer of carbocyclic-2',3'-didehydro-2',3'-dideoxyguanosine, has been shown to be the biologically active form for the inhibition of human immunodeficiency virus type 1 replication. The concentration of (-)-carbovir required to reduce reverse transcriptase activity by 50% compared with the control was 0.8 microM in H9 cells infected with the HTLV-IIIb strain; the 50% inhibitory concentration for cytotoxicity was greater than 2 mM in these cells. The effect of the timing of drug addition, pre- and postinfection, and the effect of increasing amounts of virus on the antiviral activities of (-)-carbovir and 3'-azido-3'-deoxythymidine were determined.
authors.affiliation: Division of Chemotherapy, Glaxo Research Laboratories, Glaxo Inc., Research Triangle Park, North Carolina 27709.
authors.forename: S G
authors.initials: SG
authors.lastname: Carter
authors.forename: J A
authors.initials: JA
authors.lastname: Kessler
authors.forename: C D
authors.initials: CD
authors.lastname: Rankin
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D015224:Dideoxynucleosides / Q000494:pharmacology*; D006678:HIV / Q000187:drug effects*; D006801:Humans; D014779:Virus Replication / Q000187:drug effects; D015215:Zidovudine / Q000494:pharmacology*
chemical_list: D000998:Antiviral Agents; D015224:Dideoxynucleosides; C057503:carbovir; D015215:Zidovudine