Structure of an interferon-alpha 2 gene expressed in the bovine conceptus early in gestation.
Genomic and cDNA clones for bovine trophoblast interferon (IFN) have been isolated by probing a bovine genomic library and a bovine embryonic (day-18 post coitus) cDNA library respectively with the ovine trophoblast IFN cDNA. The two DNA sequences were identical; sequence analysis demonstrated 80% identity between the amino acid sequence of bovine trophoblast IFN and ovine trophoblast IFN, and 70% identity with a previously identified bovine IFN-alpha 2. Southern blotting of bovine genomic DNA indicated the presence of a minimum of three trophoblast IFN genes. Primer extension analysis identified the transcription start site in the 5' flanking region of the bovine IFN gene. Computer-aided analysis of the 5' flanking sequence demonstrated a similarity with that of bovine IFN-alpha 2 and the existence of a possible viral induction sequence.
Stewart
H J
HJ
AFRC Research Group on Hormones and Farm Animal Reproduction, Institute of Animal Physiology and Genetics Research, Cambridge.
McCann
S H
SH
Flint
A P
AP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002417:Cattle; D004622:Embryo, Mammalian; D015870:Gene Expression; D005865:Gestational Age; D007370:Interferon Type I / Q000235:genetics*; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D014327:Trophoblasts / Q000378:metabolism*
D007370:Interferon Type I