High performance liquid chromatography methods for studying protein binding of aluminium in human serum in the absence and in the presence of desferrioxamine.
Ion-exchange high performance liquid chromatography of serum proteins was combined with aluminium determination by electrothermal-atomisation-atomic-absorption spectroscopy and fluorimetry for studying the distribution of aluminium in human serum in the absence and in the presence of desferrioxamine. Aluminium was eluted as a single peak in the same fraction as transferrin. However, following the addition of desferrioxamine most of the aluminium was liberated from transferrin and become attached to the chelator.
García Alonso
J I
JI
Department of Physical and Analytical Chemistry, Universidad de Oviedo, Spain.
López García
A
A
Pérez Parajón
J
J
Blanco González
E
E
Sanz Medel
A
A
Cannata
J B
JB
D000535:Aluminum / Q000097:blood*; D002614:Chelating Agents / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D003676:Deferoxamine / Q000494:pharmacology*; D005470:Fluorometry / Q000379:methods; D006801:Humans; D011485:Protein Binding; D012709:Serum Albumin / Q000032:analysis; D014168:Transferrin / Q000032:analysis
D002614:Chelating Agents; D012709:Serum Albumin; D014168:Transferrin; D000535:Aluminum; D003676:Deferoxamine