title: MHC and non-MHC-restricted recognition of filarial surface antigens in mice transplanted with adult Brugia malayi parasites.
abstract: We describe here the genetic control of humoral responses to filarial nematode Ag elicited by live adult Brugia malayi parasites in mice. Inbred and congenic mice of two different MHC haplotypes, H-2k and H-2d, were examined. Serologic analysis showed that the humoral responses to the major surface 29-kDa glycoprotein of adult parasites and a 40-kDa Ag from the surface of the microfilarial stage were restricted to mice with H-2k alleles (B10.BR, CBA/Ca, and CBA/N), whereas mice of the H-2d haplotype (B10.D2/n and BALB/c) were nonresponsive to these Ag. Conversely, internal adult Ag of molecular mass of 24 and 66 kDa were recognized only by animals with the H-2d haplotype. Apart from MHC-restricted recognition, the level of responses to phosphorylcholine and to a 15-kDa adult surface molecule were found to be influenced by non-MHC genes. A sharp restriction was also observed to an adult surface Ag complex of 17 to 200 kDa, which was recognized only by BALB/c mice. Thus, multiple examples of both H-2 and background genetic effects on the immune response to distinct filarial Ag can be found.
authors.affiliation: Wellcome Research Centre for Parasitic Infections, Department of Biology, Imperial College of Science, London, United Kingdom.
authors.forename: G E
authors.initials: GE
authors.lastname: Kwan-Lim
authors.forename: R M
authors.initials: RM
authors.lastname: Maizels
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D000917:Antibody Formation; D000947:Antigens, Helminth / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D015153:Blotting, Western; D002009:Brugia / Q000276:immunology*; D004605:Elephantiasis, Filarial / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D007153:Immunologic Deficiency Syndromes / Q000276:immunology; D008285:Major Histocompatibility Complex; D051379:Mice; D008970:Molecular Weight; D011233:Precipitin Tests
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D000954:Antigens, Surface