Fibronectin induces migration of bovine trabecular meshwork cells in vitro.
The migration of cultured bovine trabecular meshwork cells in response to fibronectin was studied using a 48-well migration chamber. Fibronectin was shown to be a potent chemoattractant for these cells, with a dose optimum of 30 micrograms ml-1. At this dose, the number of migrated cells was approximately 12 times that of the control, which was serum-free medium. Analysis of the migratory response revealed it to be mainly a chemokinesis, but chemotaxis was a substantial component. Electron microscopy demonstrated that adhesion and spreading of cells on the surface of the pore-containing membrane are necessary prerequisites to their migration through a pore. Microspikes are abundant during the movement of cellular processes into and through the pores. The migrated cells are extremely thin with a large surface area, but despite this phenotypic alteration the ultrastructure of migrated cells is unchanged.
Calthorpe
C M
CM
Institute of Ophthalmology, London, U.K.
Grierson
I
I
D000818:Animals; D002417:Cattle; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D005353:Fibronectins / Q000494:pharmacology*; D008854:Microscopy, Electron; D014129:Trabecular Meshwork / Q000187:drug effects* / Q000648:ultrastructure
D005353:Fibronectins