Non-invasive quantitation of human liver metabolites using image-guided 31P magnetic resonance spectroscopy.
Phosphorus-containing metabolites in normal human liver have been quantitated non-invasively with 31P magnetic resonance spectroscopy using surface coils. The location of the volume of interest (VOI) was defined by 1H magnetic resonance imaging. Subsequently, a modified three-dimensional localization technique (ISIS) was used to acquire 31P magnetic resonance spectra from the VOI. To account for partial saturation produced by rapid signal averaging, the spin/lattice relaxation times (T1) of all hepatic phosphorus resonances were measured. The corrected resonance integrals were used to derive absolute molar concentrations for the following hepatic metabolites (mmol/kg wet weight): ATP, 2.0; inorganic phosphate, 2.1; phosphodiesters, 5.4; and phosphomonoesters, 0.9. These values are compared with previously reported values for humans using freeze-clamping techniques, and provide a basis for comparison with studies of hepatic disease in this laboratory.
Meyerhoff
D J
DJ
Magnetic Resonance Unit, Veterans Administration Medical Center, San Francisco, CA.
Karczmar
G S
GS
Matson
G B
GB
Boska
M D
MD
Weiner
M W
MW
D000255:Adenosine Triphosphate / Q000032:analysis; D000328:Adult; D004952:Esters / Q000032:analysis; D005260:Female; D006801:Humans; D008099:Liver / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D008875:Middle Aged; D010710:Phosphates / Q000032:analysis; D010758:Phosphorus
D004952:Esters; D010710:Phosphates; D010758:Phosphorus; D000255:Adenosine Triphosphate