Removal of 2',3'-dideoxynucleotide residues from injected DNA in Xenopus laevis oocytes.
Dideoxynucleotides have proved to be potent differential inhibitors of DNA polymerases in vitro and in vivo. Used extensively in studies of DNA repair and replication, they have more recently been used as antiviral agents particularly in treating patients for acquired immunodeficiency syndrome (AIDS). Once incorporated, these sugar-modified analogues prevent the further extension of the polynucleotide chain because of the absence of a 3'-hydroxyl group. We demonstrated that, upon injection into Xenopus laevis oocytes, 2',3'-dideoxynucleotides are efficiently removed from plasmid DNA preterminated with these analogues allowing subsequent closure by ligation. The removal process is not sensitive to aphidicolin but is quantitatively inhibited by novobiocin.
Legerski
R J
RJ
Department of Molecular Genetics, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Penkala
J E
JE
Peterson
C A
CA
Wright
D A
DA
D000818:Animals; D001345:Autoradiography; D004260:DNA Repair; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D003838:Deoxyadenine Nucleotides / Q000378:metabolism; D015224:Dideoxynucleosides / Q000378:metabolism*; D054306:Dideoxynucleotides; D005260:Female; D007700:Kinetics; D009865:Oocytes / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D010957:Plasmids; D014176:Protein Biosynthesis; D014982:Xenopus laevis
D003838:Deoxyadenine Nucleotides; D015224:Dideoxynucleosides; D054306:Dideoxynucleotides; D010761:Phosphorus Radioisotopes; C037773:2',3'-dideoxyadenosine triphosphate; D004259:DNA-Directed DNA Polymerase