The prosomal protein of 27 kDa and a nuclear 38 kDa protein are immunologically related.
A monoclonal antibody, recognizing the prosomal 27 kDa protein, was used to detect prosomal proteins in nuclear fractions. Protein gel electrophoresis and immunoblotting revealed large amounts of a 38 kDa protein and in very low amount a 27 kDa protein reacting with the monoclonal antibody. Immunoblot analysis of V8 proteolytic fragments demonstrated that both proteins are intimately related and suggest that the nuclear 38 kDa protein could be a precursor of the 27 kDa protein (pros P 27) which is a structural component of cytoplasmic prosomes.
Kreutzer-Schmid
C
C
Biologisches Institut, Universität Stuttgart, FRG.
Schmid
H P
HP
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000941:Antigens / Q000276:immunology; D015153:Blotting, Western; D003593:Cytoplasm / Q000276:immunology*; D006367:HeLa Cells; D006801:Humans; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000276:immunology*; D010449:Peptide Mapping; D012261:Ribonucleoproteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000941:Antigens; D009687:Nuclear Proteins; D012261:Ribonucleoproteins