Labeling of proteins using [105Rh]Rh-4-(p-aminobenzyl)-diethylenetriamine.
A bifunctional ligand 4-(p-aminobenzyl)-diethylenetriamine has been synthesized. 105Rh complexes with this ligand were prepared with an overall yield of 79% at pH 9.0 in bicarbonate buffer. The preformed complex was converted to the isothiocyanate derivative using thiophosgene. Conjugation yields of 75% with IgG and 85% with HSA could be obtained for a 4 h conjugation reaction. Affinity chromatography of human-IgG coupled to the rhodium complex in an anti-human IgG agarose gel indicated no denaturation of the labeled protein. The procedure reported here can be adapted for the preparation of 105Rh-labeled antibodies for therapeutic applications.
Pillai
M R
MR
Department of Chemistry, University of Missouri, Columbia 65211.
Lo
J M
JM
John
C S
CS
Troutner
D E
DE
D007074:Immunoglobulin G; D007553:Isotope Labeling / Q000379:methods; D009942:Organometallic Compounds; D011073:Polyamines / Q000138:chemical synthesis*; D011868:Radioisotopes; D012238:Rhodium; D012709:Serum Albumin
D007074:Immunoglobulin G; D009942:Organometallic Compounds; D011073:Polyamines; D011868:Radioisotopes; D012709:Serum Albumin; C065270:rhodium 4-(4-aminobenzyl)diethylenetriamine; D012238:Rhodium