Inhibition of carnitine palmitoyltransferase leads to induction of 3-hydroxymethylglutaryl coenzyme A reductase activity in rat liver.
The relation between carnitine palmitoyltransferase (CPT) activity and 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase activity was investigated. Rats were treated with aminocarnitine or 1-carnitine overnight. In rats, in which CPT activity was inhibited by aminocarnitine, plasma and hepatic triacylglycerol contents were increased 5- to 6-fold. The plasma cholesterol concentration was unchanged, while the hepatic cholesterol content was lowered (-16%). Hepatic cholesterol synthesis, determined by following the incorporation of 14C-acetate and 3H2O into digitonin-precipitable sterols, in liver slices was increased 5- to 7-fold. HMG-CoA reductase activity in liver microsomes was increased to the same extent.
Jansen
H
H
Department of Biochemistry, Medical Faculty, Erasmus University Rotterdam, The Netherlands.
Hoogerbrugge Van der Linden
N
N
Hülsmann
W C
WC
D000217:Acyltransferases / Q000037:antagonists & inhibitors*; D000818:Animals; D001622:Betaine / Q000031:analogs & derivatives / Q000494:pharmacology; D002331:Carnitine / Q000494:pharmacology; D002334:Carnitine O-Palmitoyltransferase / Q000037:antagonists & inhibitors*; D002784:Cholesterol / Q000097:blood / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D006903:Hydroxymethylglutaryl CoA Reductases / Q000096:biosynthesis*; D008099:Liver / Q000201:enzymology*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D014280:Triglycerides / Q000097:blood / Q000378:metabolism
D014280:Triglycerides; D001622:Betaine; D002784:Cholesterol; C045761:emeriamine; D006903:Hydroxymethylglutaryl CoA Reductases; D000217:Acyltransferases; D002334:Carnitine O-Palmitoyltransferase; D002331:Carnitine