title: Species tropism of HIV-1 infectivity of interspecific cell hybridomas implies non-CD4 structures are required for cell entry.
abstract: Studies have demonstrated that CD4 may be necessary but not sufficient for human immunodeficiency virus (HIV) infection. Murine cell lines constructed to express the human CD4 glycoprotein demonstrate a restriction in their ability to support infection by the HIV-1 retrovirus. Such restrictions may indicate that molecules in addition to CD4 may be necessary for HIV-1 infection. HIV-1 infectivity has been examined in a panel of murine and human cell lines. It has been demonstrated that CD4 expression allows infectivity on a wide range of human cell lines. Some interspecific hybridomas constructed between human and murine cell lines can, when expressing the human CD4 glycoprotein, become infectable in a manner similar to human cell lines. These studies imply that surface molecules, in addition to CD4, are necessary for HIV-1 entry and infectivity. Such molecules will be important for understanding the pathogenesis of HIV-1.
authors.affiliation: University of Pennsylvania, Philadelphia 19104-6082.
authors.forename: D B
authors.initials: DB
authors.lastname: Weiner
authors.forename: K
authors.initials: K
authors.lastname: Hubner
authors.forename: W V
authors.initials: WV
authors.lastname: Williams
authors.forename: M I
authors.initials: MI
authors.lastname: Greene
mesh_terms: D000818:Animals; D015704:CD4 Antigens / Q000378:metabolism*; D002460:Cell Line; D015699:HIV Envelope Protein gp120 / Q000378:metabolism; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D006825:Hybridomas / Q000378:metabolism / Q000382:microbiology*; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D011485:Protein Binding; D011991:Receptors, Virus / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D015704:CD4 Antigens; D015699:HIV Envelope Protein gp120; D008565:Membrane Proteins; D011991:Receptors, Virus