Cloning of a lincosamide resistance determinant from Streptomyces caelestis, the producer of celesticetin, and characterization of the resistance mechanism.
Self-resistance has been investigated in Streptomyces caelestis (producer of the lincosamide antibiotic celesticetin), from which a lincosamide resistance determinant (clr) has been isolated on a 1-kilobase DNA fragment and cloned in Streptomyces lividans. The clr product is a specific methylase which produces a single residue of N6-monomethyladenine in 23S rRNA at position 2058, thereby rendering the 50S ribosmal subunit resistant to the action of lincosamides.
Calcutt
M J
MJ
Department of Biochemistry, University of Leicester, England.
Cundliffe
E
E
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000494:pharmacology*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics*; D005798:Genes, Bacterial; D007700:Kinetics; D008034:Lincomycin / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000494:pharmacology; D055231:Lincosamides; D018942:Macrolides; D008826:Microbial Sensitivity Tests; D012338:RNA, Ribosomal, 23S / Q000235:genetics / Q000302:isolation & purification; D015183:Restriction Mapping; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism; D013302:Streptomyces / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D055231:Lincosamides; D018942:Macrolides; D012338:RNA, Ribosomal, 23S; C004650:celesticetin A; D008034:Lincomycin