Multiple proteins bind to the P2 promoter region of the zein gene pMS1 of maize.
A 216 bp promoter fragment of the 19 kDa protein zein gene pMS1, containing the CCAAT and TATA boxes, was analysed by a variety of techniques for in vitro interactions with nuclear proteins from endosperm tissue. HMG proteins were found to form stable complexes with these A/T-rich promoter sequences and several specific DNA-binding proteins appear to be involved in the formation of DNA-protein complexes with this fragment. A 29 bp region spanning the two CCAAT boxes was protected from DNase I digestion in footprinting experiments.
Maier
U G
UG
Institute of Biology III, Freiburg, Federal Republic of Germany.
Grasser
K D
KD
Haass
M M
MM
Feix
G
G
D001483:Base Sequence; D002846:Chromatography, Affinity; D004268:DNA-Binding Proteins / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D017343:Genes, Plant; D006609:High Mobility Group Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D003313:Zea mays / Q000235:genetics*; D015028:Zein / Q000235:genetics*
D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; D015028:Zein; D003850:Deoxyribonuclease I