Purification of alpha-L-fucosidase by C-glycosylic affinity chromatography, and the enzymic synthesis of alpha-L-fucosyl disaccharides.
An alpha-L-fucosidase from porcine liver was purified using the new C-glycosylic affinity adsorbent, Sepharose-epsilon-aminocaproyl-3-(alpha-L-fucopyranosyl)propylamine. The C-fucosylic linkage was synthesized by a radical reaction of 2,3,4-tri-O-acetyl-alpha-L-fucopyranosyl bromide with acrylonitrile. In transglycosylation reactions with p-nitrophenyl alpha-L-fucopyranoside or alpha-L-fucopyranosyl fluoride as donor and methyl beta-D-galactopyranoside as acceptor, the enzyme mediated the formation of (1----2)- and (1----6)-linked alpha-L-fucosyl derivatives (6.5 and 10.0%, respectively).
Svensson
S C
SC
Organisch-Chemisches Institut, Universität Münster, F.R.G.
Thiem
J
J
D000818:Animals; D002240:Carbohydrate Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D002846:Chromatography, Affinity; D004187:Disaccharides / Q000096:biosynthesis; D006031:Glycosylation; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D005644:alpha-L-Fucosidase / Q000302:isolation & purification*
D004187:Disaccharides; D005644:alpha-L-Fucosidase