Amnion cell prostaglandin E2 stimulatory activity in chorion laeve-conditioned medium.
In this study we demonstrate the presence of a stimulant(s) to amnion cell prostaglandin (PG) E2 production in chorion-conditioned medium (CCM). The CCM induced a dose-dependent increase in amnion cell PGE2 production. This stimulatory activity was eliminated by heat and protease treatment. Maximal stimulation of amnion PGE2 by CCM did not occur until after 2 h of incubation, and treatment with cycloheximide (1 microgram/ml) effectively eliminated the ability of the amnion cells to respond to CCM. Additionally, CCM and arachidonic acid (2-40 microM) were synergistic in their stimulatory actions on amnion PGE2 production. CCM-treated amnion cells recover more quickly from acetylsalicylic acid pretreatment as compared to control. It is concluded that CCM contains a heat-labile protein which stimulates amnion cell PGE2 production by induction of prostaglandin endoperoxide synthase activity.
Lundin-Schiller
S
S
Department of Obstetrics and Gynecology, University of Utah, Salt Lake City 84132.
Gibb
W
W
Mitchell
M D
MD
D000650:Amnion / Q000187:drug effects / Q000378:metabolism*; D001095:Arachidonic Acids / Q000008:administration & dosage / Q000494:pharmacology; D001241:Aspirin / Q000494:pharmacology; D002478:Cells, Cultured; D002823:Chorion / Q000187:drug effects / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology; D015232:Dinoprostone / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005260:Female; D006801:Humans; D007700:Kinetics; D011247:Pregnancy
D001095:Arachidonic Acids; D003513:Cycloheximide; D015232:Dinoprostone; D001241:Aspirin