mRNA of bovine tissue inhibitor of metalloproteinase: sequence and expression in bovine ovarian tissue.
A cDNA library derived from poly(A+)RNA of bovine ovary was screened with a PCR fragment comprising the coding region of human tissue inhibitor of metalloproteinase inhibitor (TIMP). From a number of positive clones, pBGR19, containing a 747 bp insert, was identified and sequenced. The derived amino acid sequence represents that of the precursor of bovine TIMP. Northern analysis reveals a TIMP specific mRNA of 800 bp. Southern analysis indicates that one gene appears to specify bovine TIMP. TIMP mRNA is only weakly expressed in follicular granulosa- and theca cells, whereas luteinization of the follicle is associated with an increase of expression. Expression varies with the stage of the luteal phase; it was highest in stages I and III, but low in stages II and IV of the oestrous cycle.
Freudenstein
J
J
Max-Planck-Institut für Biophysikalische Chemie, Abt. Molekulare Biologie, Göttingen, W. Germany.
Wagner
S
S
Luck
R M
RM
Einspanier
R
R
Scheit
K H
KH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002417:Cattle; D003001:Cloning, Molecular; D005260:Female; D006023:Glycoproteins / Q000235:genetics*; D006107:Granulosa Cells / Q000502:physiology; D008969:Molecular Sequence Data; D010053:Ovary / Q000033:anatomy & histology / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D019714:Tissue Inhibitor of Metalloproteinases
D006023:Glycoproteins; D012333:RNA, Messenger; D019714:Tissue Inhibitor of Metalloproteinases