Taenia taeniaeformis: inactivation of metacestodes by gossypol in vitro.
Gossypol, a natural biphenyl compound inhibits Taenia taeniaeformis metacestode development in vivo. In this paper, the direct effect of gossypol on metacestodes was examined. Within 24 hr of incubation at 37 degrees C in greater than or equal to 10(-5) M gossypol, shedding of the tegument from the surface of the metacestodes was observed. There was a significant decrease in [3H]thymidine uptake by T. taeniaeformis in greater than or equal to 10(-5)M gossypol. In addition, NADH lactate dehydrogenase activity of metacestodes was significantly inhibited in greater than or equal to 10(-5) M gossypol. Thus, gossypol has a direct inhibitory effect on T. taeniaeformis metacestodes in vitro.
Rikihisa
Y
Y
Department of Veterinary Pathobiology, College of Veterinary Medicine, Ohio State University, Columbus 43210.
Lin
Y C
YC
Garber
P L
PL
Gu
Y
Y
D000818:Animals; D006072:Gossypol / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000037:antagonists & inhibitors; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D013621:Taenia / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development / Q000648:ultrastructure
D007770:L-Lactate Dehydrogenase; D006072:Gossypol