Novobiocin-resistance sequences from the novobiocin-producing strain Streptomyces niveus.
Two distinct DNA sequences expressing novobiocin resistance in Streptomyces lividans were cloned from the novobiocin-producing species Streptomyces niveus. Clone pGL101 (5kb) conferred resistance to 50 micrograms ml-1 novobiocin, whereas clones pGL102 and pGL103, which carry the same 6.5kb insert but in opposite orientations, expressed resistance to 150 micrograms ml-1. The cloned inserts from pGL101 and pGL103 failed to hybridize with each other or with the cloned novobiocin-resistant gyrB sequence from Streptomyces sphaeroides. Both probes hybridized strongly with DNA from the novobiocin-producing species S. niveus and S. sphaeroides but no hybridization (pGL103) or very weak hybridization (pGL101) was detected with DNA from the non-producing species S. lividans, Streptomyces griseus and Streptomyces antibioticus. S. niveus contains at least three novobiocin-resistance determinants with the pGL101 and pGL103 cloned sequences specific for novobiocin-producing strains of Streptomyces.
Mitchell
J I
JI
Department of Genetics and Microbiology, University of Liverpool, Donnan Laboratories, UK.
Logan
P G
PG
Cushing
K E
KE
Ritchie
D A
DA
D015139:Blotting, Southern; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis; D004352:Drug Resistance, Microbial / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D009675:Novobiocin / Q000494:pharmacology*; D015183:Restriction Mapping; D013302:Streptomyces / Q000187:drug effects / Q000235:genetics*; D013329:Structure-Activity Relationship
D004269:DNA, Bacterial; D009675:Novobiocin