title: Receptor-mediated endocytosis of low-density lipoprotein by cultured human glomerular cells.
abstract: Lipoproteins might be involved in the pathogenesis of glomerular damage. Uptake of low-density lipoprotein (LDL) by cultured human glomerular cells has been studied using LDL, labelled with the fluorescent probe 1,1'-dioctadecyl-3,3,3'3'-tetramethyl-indocarbocyanine perchlorate (diI). Cells have been characterised using phase-contrast microscopy, monoclonal antibodies and lectins. Differentiated glomerular epithelial cells, epithelial-like cells and mesangial cells all took up diI-LDL. Uptake was specific for LDL, of high affinity and inhibited by excess unlabelled LDL, heparin and preloading the cells with cholesterol. Binding of diI-LDL to the cell surface was restricted to discrete areas which were arranged in linear arrays on mesangial cells. Endocytosis of surface-bound diI-LDL occurred within 3 min and breakdown of internalised diI-LDL within 30 min. These results indicate that cultured human glomerular cells take up LDL by receptor-mediated endocytosis.
authors.affiliation: Department of Nephrology, Leicester General Hospital, UK.
authors.forename: H C
authors.initials: HC
authors.lastname: Rayner
authors.forename: T
authors.initials: T
authors.lastname: Horsburgh
authors.forename: S L
authors.initials: SL
authors.lastname: Brown
authors.forename: F L
authors.initials: FL
authors.lastname: Lavender
authors.forename: A F
authors.initials: AF
authors.lastname: Winder
authors.forename: J
authors.initials: J
authors.lastname: Walls
mesh_terms: D002478:Cells, Cultured; D004705:Endocytosis; D004848:Epithelium / Q000378:metabolism; D006801:Humans; D007678:Kidney Glomerulus / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D011973:Receptors, LDL / Q000502:physiology*
chemical_list: D008077:Lipoproteins, LDL; D011973:Receptors, LDL