A spectroelectrochemical study of microperoxidase at bare and gold-plated RVC thin-layer electrodes.
Direct electrochemistry of microperoxidase (the heme-undecapeptide from cytochrome c) has been followed at a bare and a gold plated RVC thin-layer electrode, using the spectropotentiostatic method or voltabsorptometry. Both techniques yield 'clean' and undistorted signals; their analysis easily provides quantitative information for the electrochemical parameters of microperoxidase and shows that spectroelectrochemistry is a powerful method to study the redox behavior of metalloproteins or their active site fragments.
Zamponi
S
S
Department of Chemistry, University of Camerino, Italy.
Santucci
R
R
Brunori
M
M
Marassi
R
R
D002244:Carbon; D004563:Electrochemistry; D004566:Electrodes; D006046:Gold; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D010544:Peroxidases; D013053:Spectrophotometry
D002244:Carbon; D006046:Gold; D010544:Peroxidases; C021799:microperoxidase