title: [A method for more accurate determination of the properties of antinuclear antibodies].
abstract: The aim of the study was to evaluate statistically the result of 18 accessory reactions performed histochemically on cryostat tissue sections with patient sera exhibiting positive immunofluorescent reaction with antigens of the cell nucleus in the dilution of 1:10. The following antisera were used: antisera to immunoglobulin classes, A, D, E, G, and M, to complement components C3, C4, and B1AC, to immunoglobulin fragments Fc, Fc + Fc', and Fab, as well as species-nonspecific antisera SWAR and RASw. The immunofluorescent reaction to antinuclear antibodies was performed on sections pretreated with physiological saline, HCl, trypsin, DNase and RNase. A total of 152 sera was thus examined. Further detailed analysis is presumed to provide a basis for developing a system of extended diagnostic possibilities in the field of autoaggressive diseases.
authors.affiliation: Oddelenie patológie NsP, OUNZ, Presov.
authors.forename: A
authors.initials: A
authors.lastname: Bayer
authors.forename: D
authors.initials: D
authors.lastname: Palcáková
mesh_terms: D000818:Animals; D000974:Antibodies, Antinuclear / Q000032:analysis* / Q000276:immunology; D006168:Guinea Pigs; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D013045:Species Specificity
chemical_list: D000974:Antibodies, Antinuclear
vernacular_title: Príspevok k metodike blizsieho urcenia vlastností antinukleárnych protilátok.