Restoration of the responsiveness to growth factors in senescent cells by an embryonic cell extract.
In senescent fibroblast cell cultures which have approached a postmitotic stage in vitro, responsiveness to growth factors is restored upon exposure to an embryonic sheep cell extract. The extract contains molecules below a molecular weight of 1 x 10(5) Da in aqueous solution. Following a transient exposure to the extract, mitotic activity is resumed, and the cells keep dividing over several passages. The target cells which respond to the treatment were identified in a single-cell assay as those that still had the capacity to undergo at least several mitotic divisions before entering the final stage of senescence.
Amtmann
E
E
Institute for Virus Research, German Cancer Research Center, Heidelberg.
Eddé
E
E
Sauer
G
G
Westphal
O
O
D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D006224:Cricetinae; D003470:Culture Media; D004622:Embryo, Mammalian / Q000502:physiology*; D006133:Growth Substances / Q000494:pharmacology; D007700:Kinetics; D012756:Sheep; D014020:Tissue Extracts / Q000494:pharmacology*
D003470:Culture Media; D006133:Growth Substances; D014020:Tissue Extracts