Nude mouse resists hepatic metastasis of the allogeneic tumor, colon-26.
The ability of the host-immune defense mechanism of nude mice and their immunocompetent littermates to prevent liver metastases from the murine colon carcinoma, colon-26, was assessed. Give thousand tumor cells suspended in 0.05 ml of Hank's balanced salt solution were inoculated into the spleens of BALB/c nu/+ and BALB/c nu/nu mice. On the 21st day after inoculation, all the mice were sacrificed, and the liver metastases counted and the livers weighed. All the BALB/c nu/+ mice were found to have developed hepatic metastases with a mean of 10 nodules, whereas no hepatic metastases were observed in any of the 10 BALB/c nude mice. On the other hand, 4 of 6 nude mice developed hepatic metastases after treatment with anti-asialo GM1 antibody. These results indicate that the BALB/c nude mouse has an excellent host-immune defense mechanism for preventing liver metastasis, with NK cells in the liver and/or blood circulation perhaps playing an important role.
Arisawa
Y
Y
Department of Surgery, School of Medicine, Keio University, Tokyo, Japan.
Kubota
T
T
Suto
A
A
Kodaira
S
S
Ishibiki
K
K
Abe
O
O
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage; D003110:Colonic Neoplasms / Q000601:surgery*; D005069:Evaluation Studies as Topic; D005677:G(M1) Ganglioside; D006028:Glycosphingolipids / Q000276:immunology; D007113:Immunity, Innate; D007694:Killer Cells, Natural / Q000032:analysis; D008113:Liver Neoplasms / Q000276:immunology / Q000517:prevention & control* / Q000556:secondary; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology*; D008819:Mice, Nude / Q000276:immunology*; D013997:Time Factors; D014184:Transplantation, Homologous
D000906:Antibodies; D006028:Glycosphingolipids; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside