title: C1q receptor on murine cells.
abstract: Different cells and cell lines of murine origin were tested for their capacity to bind the C subcomponent C1q by using biotinylated human C1q and streptavidin-FITC. Cytofluorometric analysis of splenocytes and thymocytes shows that the majority of C1q-reactive cells reside in the population of B cells and macrophages. There is a significant difference in the C1q-binding capacity of in vitro activated cells; although more than half of the B cell blasts bind the C subcomponent, T cell blasts are virtually negative. It is shown that pre-B lymphomas and cell lines of myeloid origin bind C1q strongly (90 to 98%), whereas in the case of mature B cell lymphomas, plasmocytomas, and the tested T cell lines, the percentage of C1q binding cells varies from 0 to 56. C1q affinity chromatography of the detergent extracts from P388D1 and WEHI-3 cells followed by SDS-PAGE of the eluted proteins under reducing conditions reveals a band at approximately 80 kDa. Analysis of splenocytes shows two additional minor C1q-binding molecules with apparent molecular masses of 50 and 45 kDa, whereas in the case of B cell blasts three bands of similar density are seen at approximately 95, 50 and 45 kDa. C1q-receptors of murine cells are shown to be antigenically related to their human counterpart, because a polyclonal antibody (266A) raised against the human C1q receptor reacts with them.
authors.affiliation: Basel Institute for Immunology, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Erdei
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D002846:Chromatography, Affinity; D003429:Cross Reactions; D005434:Flow Cytometry; D018960:Hyaluronan Receptors; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D008562:Membrane Glycoproteins; D051379:Mice; D008815:Mice, Inbred Strains; D024101:Mitochondrial Proteins; D008970:Molecular Weight; D009000:Monocytes / Q000378:metabolism; D011233:Precipitin Tests; D011951:Receptors, Complement / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology
chemical_list: C490193:C1qbp protein, mouse; D018960:Hyaluronan Receptors; D008562:Membrane Glycoproteins; D024101:Mitochondrial Proteins; D011951:Receptors, Complement; C032402:complement 1q receptor