6-aminohexanoate and chloride ion in the activation by urokinase of porcine plasminogens.
The rate of activation by urokinase of porcine plasminogen is accelerated by 6-aminohexanoate, although the maximally enhanced rate is 10-fold less than that of human plasminogen without the amino acid. 6-Aminohexanoate facilitates only activation of native porcine plasminogen (asp-plasminogen), but has no effect on activation of des-kringle1-4-plasminogen. Sodium chloride, on the other hand, inhibits activation by urokinase of both porcine asp-plasminogen and des-kringle1-4-plasminogen. It is concluded that 6-aminohexanoate exerts its effect via kringle1-4 domains of plasminogen, whereas Cl- acts, at least in part, through effects on the kringle5 or proteinase domains.
Machovich
R
R
Section of Hematology Research, Mayo Clinic and Foundation, Rochester, MN 55905.
Owen
W G
WG
D000614:Aminocaproates / Q000494:pharmacology*; D015119:Aminocaproic Acid / Q000494:pharmacology*; D000818:Animals; D002712:Chlorides / Q000494:pharmacology*; D004789:Enzyme Activation; D006801:Humans; D007700:Kinetics; D009994:Osmolar Concentration; D010958:Plasminogen / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D013552:Swine; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism*
D000614:Aminocaproates; D002712:Chlorides; D012965:Sodium Chloride; D010958:Plasminogen; D014568:Urokinase-Type Plasminogen Activator; D015119:Aminocaproic Acid