HCl secretion and [Ca2+]i in cultured parietal cells.
Gastric parietal cells in primary culture have been tested to determine their utility as a model for the study of the role of intracellular calcium ([Ca2+]i) in the control of HCl secretion. Changes in [Ca2+]i were measured in single cells on a microscope stage using the cell-permeant form of the fluorescent calcium probe, fura-2. Simultaneous images of cell fluorescence and morphology were acquired using a digitized video image analysis system and two video cameras, one for low-light level fluorescence detection and one for high resolution DIC/transmitted light images. Both histamine and carbachol, which are known stimulants of HCl secretion, increased [Ca2+]i and stimulated dramatic changes in morphology in these cultured cells. Changes in morphology were accompanied by an increased uptake of the weak base, [14C]-aminopyrine (AP), and a shift from green to red fluorescence of another weak base, acridine orange. These results indicate that cultured parietal cells, maintained under controlled conditions on a microscope stage, retain viability and secretagogue responsiveness. Thus, this cellular model appears to be suitable for correlation of changes in [Ca2+]i and activation of HCl secretion.
Chew
C S
CS
Department of Physiology, Morehouse School of Medicine, Atlanta, GA.
Ljungström
M
M
D000818:Animals; D001572:Benzofurans; D002118:Calcium / Q000378:metabolism / Q000502:physiology*; D002478:Cells, Cultured; D005456:Fluorescent Dyes; D016257:Fura-2 / Q000031:analogs & derivatives*; D005744:Gastric Acid / Q000378:metabolism*; D006632:Histamine / Q000494:pharmacology; D007091:Image Processing, Computer-Assisted; D008297:Male; D009853:Omeprazole / Q000494:pharmacology; D010295:Parietal Cells, Gastric / Q000187:drug effects / Q000378:metabolism*; D011817:Rabbits; D015290:Second Messenger Systems / Q000502:physiology; D014741:Video Recording
D001572:Benzofurans; D005456:Fluorescent Dyes; C049925:fura-2-am; D006632:Histamine; D009853:Omeprazole; D002118:Calcium; D016257:Fura-2