Water-insoluble high-molecular-weight and alpha-crystallins as the source of the Scheimpflug light scattering pattern in the rat lens.
Lenses of 14-week-old rats were separated into 10 layers or fractions by a frozen-sectioning technique. The biochemical characteristics of these layers were assigned to corresponding parts of the densitometric reading obtained from the Scheimpflug negative, which enables a correlation of light scattering values recorded in vivo to protein patterns in the same area. Calculated as percentage of lens dry weight, all water-soluble crystallins show minima and the water-insoluble crystallins show maxima in the lens nucleus. This demonstrates that the nucleus contains the bulk of the water-insoluble high-molecular-weight and alpha-crystallins, being the source for the Scheimpflug light scattering pattern.
Bours
J
J
Department of Experimental Ophthalmology, University of Bonn, FRG.
Ahrend
M H
MH
Wegener
A
A
Hockwin
O
O
D000818:Animals; D003459:Crystallins / Q000032:analysis* / Q000378:metabolism; D003720:Densitometry; D007908:Lens, Crystalline / Q000032:analysis*; D008027:Light; D008970:Molecular Weight; D010781:Photography; D051381:Rats; D012542:Scattering, Radiation; D012995:Solubility
D003459:Crystallins