Induction of peroxisome proliferation in rainbow trout exposed to ciprofibrate.
Rainbow trout (Salmo gairdneri), average body weight of 450 g, were treated with 15, 25, or 35 mg/kg of ciprofibrate via intraperitoneal injection every other day for 2 to 3 weeks. The effects on hepatic peroxisomal acyl-CoA oxidase, polypeptide PPA-80, catalase, and liver weight were measured. The treatment of trout with ciprofibrate showed significant dose-related increases in peroxisomal acyl-CoA activity, polypeptide PPA-80, and catalase after 3 weeks of exposure. Peroxisomal oxidase activity showed a significant (p = 0.0008) increase (78%) at 35 mg/kg and a marginal (p = 0.1) increase (27%) at 25 mg/kg after 3 weeks of exposure. Densitometric analysis of polypeptide PPA-80 and catalase showed increases up to 48 and 236% at 35 mg/kg, respectively. Morphometric analysis on livers of trout administered 35 mg/kg for 3 weeks showed a 2.3-fold increase of peroxisomal volume density, as compared to control. This study demonstrates the induction of peroxisome proliferation in rainbow trout administered ciprofibrate, a known peroxisome proliferator in rodents.
Yang
J H
JH
School of Public Health, University of Massachusetts, Amherst 01003.
Kostecki
P T
PT
Calabrese
E J
EJ
Baldwin
L A
LA
D042962:Acyl-CoA Oxidase; D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D001835:Body Weight / Q000187:drug effects; D002374:Catalase / Q000096:biosynthesis; D002994:Clofibrate / Q000031:analogs & derivatives*; D002995:Clofibric Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D058607:Fibric Acids; D000960:Hypolipidemic Agents / Q000494:pharmacology*; D008099:Liver / Q000033:anatomy & histology / Q000187:drug effects; D008830:Microbodies / Q000187:drug effects* / Q000648:ultrastructure; D009929:Organ Size / Q000187:drug effects; D010088:Oxidoreductases / Q000096:biosynthesis; D010452:Peptide Biosynthesis; D010455:Peptides; D013997:Time Factors; D014337:Trout
D058607:Fibric Acids; D000960:Hypolipidemic Agents; D010455:Peptides; C033176:polypeptide PPA-80; D002995:Clofibric Acid; D010088:Oxidoreductases; D002374:Catalase; D042962:Acyl-CoA Oxidase; C019304:ciprofibrate; D002994:Clofibrate