Analysis of the monohexosylceramide fraction of Echinococcus multilocularis metacestodes.
Monohexosylceramides of Echinococcus multilocularis metacestodes have been isolated and analyzed by thin-layer chromatography, gas-liquid chromatography and mass spectrometry. 90.9% of the parasite fraction was galactosylceramide; glucosylceramide was present at only 9.1%. The most important fatty acids were normal C16:0 and C26:0 fatty acids. The hydroxylated fatty acids of the ceramide part constituted 20.1% of the total, their major constituents were C18:0 and C26:0. The sphinganine accounted for 70.4% of long-chain bases, phytosphingosine and sphingosine were also detected. The importance of the long chain fatty acids and the presence of sphinganine in the monohexosylceramide fraction were discussed.
Persat
F
F
Département de Parasitologie et Pathologie Exotique, Université Claude Bernard, Lyon, France.
Bouhours
J F
JF
Mojon
M
M
Petavy
A F
AF
D000818:Animals; D002554:Cerebrosides / Q000032:analysis*; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D004446:Echinococcus / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis; D013058:Mass Spectrometry
D002554:Cerebrosides; D005227:Fatty Acids; C013870:ceramide monohexoside