Flow cytometric discrimination of human semen cells.
An improved method for differential staining and high resolution flow cytometric measurement of human semen cells is presented. Using a mild pretreatment with citric acid/detergent and staining with DAPI, the new procedure provides excellent preservation and good discrimination of all cells which are present in normal and pathological semen samples.
Otto
F J
FJ
Fachklinik Hornheide, Experimentelle Onkologie, Münster, FRG.
Hettwer
H
H
D005434:Flow Cytometry; D006801:Humans; D008297:Male; D012661:Semen / Q000166:cytology*; D013094:Spermatozoa / Q000166:cytology*