Effects of phorbol esters and secretin on pancreatic juice secretion in the anaesthetized rat.
1. An investigation was made of the effects of phorbol esters, 12-O-tetradecanoylphorbol acetate (TPA) and secretin on pancreatic juice secretion in the anaesthetized rat. TPA (10(-12)-10(-8) mol/kg body wt) evoked marked dose-dependent increases in secretory rate and total protein output. 2. An inactive phorbol ester (4 alpha-phorbol-12-13-didecanoate; 4 alpha PDD) had no effect on the secretory rate but increased total protein output compared to saline control animals. 3. When TPA was administered in combination with the protein kinase C inhibitor, Polymyxin B (10(-8) mol/kg body wt) both secretory rate and protein output were significantly reduced (P less than 0.001) compared to TPA alone. 4. Secretin (50-1600 pmol/kg body wt) increased both pancreatic juice flow and total protein output in a dose-dependent manner. 5. Simultaneous administration of secretin (50-1600 pmol/kg body wt) and TPA (10(-10) mol/kg body wt) resulted in a marked attenuation in the secretin-induced secretory rate while secretin-evoked protein output was unaffected. 6. The results indicate that protein kinase C activation is associated with pancreatic juice secretion and it may also modulate secretin-induced pancreatic juice flow in the anaesthetized rat.
Salido
G M
GM
Department of Physiology, Faculty of Veterinary Science, University of Extremadura, Caceres, Spain.
Francis
L P
LP
Camello
P J
PJ
Singh
J
J
Madrid
J A
JA
Pariente
J A
JA
D000758:Anesthesia; D000818:Animals; D005260:Female; D008297:Male; D010189:Pancreatic Juice / Q000378:metabolism*; D010703:Phorbol Esters / Q000494:pharmacology*; D011112:Polymyxin B / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012633:Secretin / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D010703:Phorbol Esters; D011506:Proteins; D012633:Secretin; D011112:Polymyxin B; D013755:Tetradecanoylphorbol Acetate