The interaction between ethanol and cysteine on the central depressant effects of ethanol in mice.
In this study male Swiss-Webster mice were used to examine the effects of cysteine (ICV), a precursor in the biosynthesis of taurine, on ethanol-induced loss of the righting reflex. The interaction of ethanol with gamma-aminobutyric acid (GABA) and isethionic acid, a metabolite of taurine, was also investigated on ethanol-induced central nervous system depression as measured by loss of the righting reflex experiments. Immediately after the animals regained the righting reflex following ethanol injection (IP) mice received an ICV injection of saline, cysteine (1, 15 or 25 mumol/kg), GABA (1, 15 or 25 mumol/kg) or isethionic acid (25 or 50 mumol/kg). Upon ICV administration of cysteine or GABA the mice again lost the righting reflex. This effect occurred immediately and in a dose-dependent manner. The compound, isethionic acid, failed to cause a second loss of the righting reflex following ethanol administration (IP). In the absence of ethanol cysteine or GABA (25 mumol/kg, ICV) did not produce a substantial loss of the righting reflex in mice. In another experiment mice were pretreated (IP) with L-2-oxothiazolide-4-carboxylate (OTC) 2 hr prior to ethanol administration (IP). OTC is a compound which can be converted to cysteine in the body. In the presence of ethanol OTC (15 mmol/kg) caused an enhancement of ethanol-induced central nervous system depression under certain conditions.(ABSTRACT TRUNCATED AT 250 WORDS)
Ferko
A P
AP
Hahnemann University, School of Medicine, Department of Pharmacology, Philadelphia, PA 19102.
D000818:Animals; D003545:Cysteine / Q000494:pharmacology*; D004357:Drug Synergism; D000431:Ethanol / Q000494:pharmacology*; D007276:Injections, Intraventricular; D007513:Isethionic Acid / Q000494:pharmacology; D008297:Male; D051379:Mice; D011187:Posture; D011761:Pyrrolidonecarboxylic Acid; D012018:Reflex / Q000187:drug effects*; D013844:Thiazoles / Q000494:pharmacology; D053778:Thiazolidines; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D013844:Thiazoles; D053778:Thiazolidines; D000431:Ethanol; D005680:gamma-Aminobutyric Acid; D007513:Isethionic Acid; D003545:Cysteine; D011761:Pyrrolidonecarboxylic Acid; C029536:2-oxothiazolidine-4-carboxylic acid