Monobiotinylated relaxins. Preparation and chemical properties of the mono(biotinyl-epsilon-aminohexanoyl) porcine relaxin.
Biotinylated porcine relaxins were synthesized and purified to homogeneity. Native porcine relaxin was reacted with biotinyl-epsilon-aminohexanoic acid-N-hydroxysuccinimide ester and the resulting mixture was separated by either ion exchange chromatography on CM-cellulose at pH 5.5 or reversed-phase high performance liquid chromatography. All four possible monosubstituted relaxin derivatives, with substitutions in N alpha A1, N epsilon A7, N epsilon A16, and N epsilon B8, were obtained. All derivatives were fully biologically active and comparative circular dichroism spectroscopy showed no significant differences in their secondary structure.
Büllesbach
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Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston.
Schwabe
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D000595:Amino Acid Sequence; D000614:Aminocaproates; D000818:Animals; D001681:Biological Assay; D001710:Biotin / Q000031:analogs & derivatives; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005260:Female; D066298:In Vitro Techniques; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D012065:Relaxin / Q000031:analogs & derivatives* / Q000032:analysis / Q000138:chemical synthesis; D013552:Swine; D014590:Uterine Contraction
D000614:Aminocaproates; D001710:Biotin; C048592:biotinyl-6-aminocaproic acid N-hydroxysuccinimide ester; D012065:Relaxin