title: The chicken oviduct and embryonic red blood cell transferrin receptors are distinct molecules.
abstract: We recently described an estrogen-inducible transferrin receptor from the chicken oviduct. We now report on the comparison of the oviduct transferrin receptor with the transferrin receptor obtained from chick embryo red blood cells. Western blot analysis reveals that rabbit polyclonal antibodies raised against one receptor do not cross react with the heterologous receptor. Furthermore, peptide map analyses of either affinity purified, native [125I]-labelled transferrin receptors (dimers) or dissociated, and repurified monomers obtained from oviducts and embryonic red blood cells yield distinct patterns. Therefore, the estrogen-modulated oviduct transferrin receptor appears to be structurally distinct from the iron-modulated red cell transferrin receptor.
authors.affiliation: Department of Biochemistry, University of Vermont College of Medicine, Burlington 05405.
authors.forename: I
authors.initials: I
authors.lastname: Poola
authors.forename: A B
authors.initials: AB
authors.lastname: Mason
authors.forename: J J
authors.initials: JJ
authors.lastname: Lucas
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002642:Chick Embryo; D002645:Chickens; D003429:Cross Reactions; D004910:Erythrocyte Membrane / Q000032:analysis*; D004967:Estrogens / Q000494:pharmacology; D008970:Molecular Weight; D010057:Oviducts / Q000032:analysis*; D010449:Peptide Mapping; D011990:Receptors, Transferrin / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D004967:Estrogens; D011990:Receptors, Transferrin