A Drosophila ribosomal protein functions in mammalian cells.
A cDNA expression vector encoding Drosophila ribosomal protein S14 was transfected into cultured Chinese hamster ovary (CHO) cells that harbor a recessive RPS14 emetine resistance mutation. Transformants synthesized the insect mRNA and polypeptide and consequently displayed an emetine-sensitive phenotype. These observations indicate that the insect protein was accurately expressed and correctly assembled into functional mammalian 40S ribosomal subunits.
Maki
C G
CG
Division of Biology, Kansas State University, Manhattan 66506.
Rhoads
D D
DD
Diaz
J J
JJ
Roufa
D J
DJ
D000818:Animals; D002460:Cell Line; D004330:Drosophila / Q000235:genetics*; D015183:Restriction Mapping; D012269:Ribosomal Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection
D012269:Ribosomal Proteins; C039747:ribosomal protein S14