Osteosarcomas in transgenic mice expressing an alpha-amylase-SV40 T-antigen hybrid gene.
Mice of a transgenic mouse lineage 501, produced by zygotic injection of the parotid alpha-amylase promoter-SV40 T-antigen hybrid gene, developed osteosarcomas at about 15 months of age. The tumors predominantly involved the axial skeleton, were sometimes multiple, and metastasized to the liver. A cell line derived from a primary tumor produced osteosarcomas on transfer to nude mice. The 501 transgenic lineage thus provides a valuable new model for studying the histogenesis of osteosarcomas.
Knowles
B B
BB
Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
McCarrick
J
J
Fox
N
N
Solter
D
D
Damjanov
I
I
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics* / Q000378:metabolism; D001859:Bone Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002460:Cell Line; D003001:Cloning, Molecular; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D012516:Osteosarcoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D011401:Promoter Regions, Genetic; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology; D000516:alpha-Amylases / Q000235:genetics* / Q000378:metabolism
D000952:Antigens, Polyomavirus Transforming; D000516:alpha-Amylases