title: Nature of the organic cofactor of pig plasma benzylamine oxidase.
abstract: The identification of the organic cofactor of pig plasma benzylamine oxidase is described. Acid hydrolysis of the enzyme in argon in the presence of phenylhydrazine (6 h at 115 degrees C in 0.027 M sulphuric acid) allowed the isolation of an adduct which was purified by HPLC and identified as phenylhydrazone of pyridoxal by spectrophotometry, spectrofluorimetry and mass spectrometry.
authors.affiliation: Department of Pharmacology, University of Florence, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Buffoni
mesh_terms: D000818:Animals; D001595:Benzylamine Oxidase / Q000097:blood* / Q000302:isolation & purification; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006868:Hydrolysis; D013058:Mass Spectrometry; D008995:Monoamine Oxidase / Q000097:blood*; D010659:Phenylhydrazines; D011732:Pyridoxal Phosphate / Q000097:blood* / Q000302:isolation & purification; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013552:Swine
chemical_list: D010659:Phenylhydrazines; C030299:phenylhydrazine; D011732:Pyridoxal Phosphate; D001595:Benzylamine Oxidase; D008995:Monoamine Oxidase