Macrophage invasion and phagocytic activity during lens regeneration from the iris epithelium in newts.
Following removal of the lens through the cornea, early stages of lens regeneration from the dorsal iris of the adult newt, Notophthalmus viridescens, were studied using light and electron microscopic observations on sectioned, plastic-embedded irises. Specimens were fixed in Karnovsky's fixative every 2 days from 0 to 12 and 15 days after lentectomy. Infiltration of the iris epithelium by macrophages and their phagocytosis of melanosomes and small fragments of iris epithelial cells were observed. These macrophages were characterized by coarse nuclear chromatin, numerous mitochondria, free ribosomes, granular endoplasmic reticulum, Golgi complexes, vesicles, lysosomes, and phagosomes containing ingested melanosomes. Lamellipodia of varying length projected from their surface. Most of the cells lying on or close to the posterior surface of the iris could be identified as macrophages by these criteria. During this period, there was enlargement of the intercellular spaces within the iris epithelium. The iris epithelial cells near the margin of the pupil elongated, lost their melanin pigment and some associated cytoplasm, and acquired abundant free polyribosomes to form a lens vesicle of depigmented cells.
Reyer
R W
RW
Department of Anatomy, School of Medicine, West Virginia University, Morgantown 26506.
D000818:Animals; D002454:Cell Differentiation; D002465:Cell Movement; D004848:Epithelium / Q000502:physiology / Q000648:ultrastructure; D007498:Iris / Q000502:physiology / Q000648:ultrastructure; D007908:Lens, Crystalline / Q000502:physiology* / Q000648:ultrastructure; D008264:Macrophages / Q000502:physiology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D010586:Phagocytes / Q000502:physiology / Q000648:ultrastructure; D010858:Pigmentation; D012038:Regeneration / Q000502:physiology*; D012455:Salamandridae; D013997:Time Factors