title: A54145, a new lipopeptide antibiotic complex: discovery, taxonomy, fermentation and HPLC.
abstract: A54145 is a complex of new lipopeptide antibiotics that inhibits Gram-positive bacteria and acts as a growth promotant for broiler chicks. Eight factors; A, B, C, D, E, F, A1 and B1; have been isolated and characterized. They contain four similar peptide nuclei, each of which is acylated with either an 2-decanoyl, n-decanoyl, or undecanoyl side chain. Taxonomic studies ascertained that the producing microorganism was a strain of Streptomyces fradiae. Fermentation studies determined that superior antibiotic yields were obtained in stirred bioreactors in a soybean flour-molasses medium employing a continuous glucose feed. These findings, interwoven with the selection of hyper-productive mutants, increased fermentation yields from less than 50 micrograms/ml to more than 1 mg/ml. An analytical HPLC system was developed for the identification and subsequent quantitation of each factor of the A54145 complex.
authors.affiliation: Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, IN 46285.
authors.forename: L D
authors.initials: LD
authors.lastname: Boeck
authors.forename: H R
authors.initials: HR
authors.lastname: Papiska
authors.forename: R W
authors.initials: RW
authors.lastname: Wetzel
authors.forename: J S
authors.initials: JS
authors.lastname: Mynderse
authors.forename: D S
authors.initials: DS
authors.lastname: Fukuda
authors.forename: F P
authors.initials: FP
authors.lastname: Mertz
authors.forename: D M
authors.initials: DM
authors.lastname: Berry
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000096:biosynthesis*; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D008074:Lipoproteins / Q000032:analysis / Q000096:biosynthesis; D008969:Molecular Sequence Data; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification / Q000378:metabolism*
chemical_list: C065208:A54145; D000900:Anti-Bacterial Agents; D008074:Lipoproteins