title: Optimal conditions for directly sequencing double-stranded PCR products with sequenase.
authors.affiliation: Biologie Moléculaire du Gène, INSERM U277, CNRS UA535, Institut Pasteur, Paris, France.
authors.forename: J L
authors.initials: JL
authors.lastname: Casanova
authors.forename: C
authors.initials: C
authors.lastname: Pannetier
authors.forename: C
authors.initials: C
authors.lastname: Jaulin
authors.forename: P
authors.initials: P
authors.lastname: Kourilsky
mesh_terms: D001483:Base Sequence; D004247:DNA; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D013696:Temperature; D013997:Time Factors
chemical_list: D004247:DNA; C034970:bacteriophage T7 induced DNA polymerase; D004259:DNA-Directed DNA Polymerase