title: [Ion-paired high performance liquid chromatographic determination of mitoxantrone hydrochloride injection].
abstract: Mitoxantrone (DHAQ) is a new anti-cancer agent. The ion-pair HPLC method to determine mitoxantrone hydrochloride injection was proposed. Mitoxantrone can be separated on Zorbax-ODS column with methanol-water-formic acid (450:50:1.5) containing 3.605g SDS as mobile phase. The pH of mobile phase was adjusted to 2.7. The flow rate was 1.0 ml/min and detection was at wavelength of 254 nm, providing a detection limit of 1ng (r/n, 3:1) of DHAQ. DHAQ was determined by peak area measurement with ametantrone as internal standard. The rectilinear calibration graph in the range of 1-10 micrograms of DHAQ was obtained (r = 0.999 2). The average recovery was 99.62 +/- 2.36% (n = 10), and the results were not interfered by the other ingredients in the injection.
authors.forename: C
authors.initials: C
authors.lastname: Wu
authors.forename: P
authors.initials: P
authors.lastname: Guo
authors.forename: H
authors.initials: H
authors.lastname: Song
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007267:Injections; D008942:Mitoxantrone / Q000032:analysis*
chemical_list: D008942:Mitoxantrone