Synthesis and radiobiological applications of [35S]L-homocysteine thiolactone.
[35S]L-Homocysteine thiolactone ([35S]L-HCTL) was synthesized and its biodistribution evaluated as a potential brain radioprotective agent and as a tissue hypoxia marker. Drug uptake in mouse brain exceeded that in s.c. tumor 3 h post injection only. Multiple indicator dilution experiments in the rabbit heart indicate that membrane permeability of [35S]L-HCTL does not limit its usefulness as a hypoxia marker. In addition, a positive correlation was observed between regional coronary blood flow and myocardial content of [35S]adenosylhomocysteine formed from [35S]homocysteine and adenosine.
Grunbaum
Z
Z
Department of Radiology, University of Washington Medical Center, Seattle 98195.
Kroll
K
K
Greene
J L
JL
Rasey
J S
JS
Krohn
K A
KA
D000818:Animals; D001921:Brain / Q000378:metabolism; D005289:Ferrets; D006710:Homocysteine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000493:pharmacokinetics; D007553:Isotope Labeling; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009206:Myocardium / Q000378:metabolism; D009374:Neoplasms, Experimental / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D011817:Rabbits; D011837:Radiation-Protective Agents / Q000138:chemical synthesis / Q000493:pharmacokinetics*; D013462:Sulfur Radioisotopes; D014018:Tissue Distribution
D011837:Radiation-Protective Agents; D013462:Sulfur Radioisotopes; D006710:Homocysteine; C007957:homocysteine thiolactone; D010100:Oxygen