title: Interpretation of microscopic image analysis of cell nuclei.
abstract: This study shows that microscopic image analyses of nuclear DNA have common characteristics among fixation methods and tissue types. We find that microscopic imaging measurements require both nuclear area and DNA concentration to properly convey diagnostic information. Algorithms are developed which enable infiltrating lymphocytes to act as internal DNA controls for each sample. The DNA content and patterns measured by microscopic imaging were found to be related to patient survival and to cytologic diagnosis.
authors.affiliation: Department of Pathology, Medical College of Wisconsin, Milwaukee 53226.
authors.forename: L L
authors.initials: LL
authors.lastname: Hause
authors.forename: L J
authors.initials: LJ
authors.lastname: Clowry
authors.forename: D S
authors.initials: DS
authors.lastname: Cheng
authors.forename: G D
authors.initials: GD
authors.lastname: Geanon
authors.forename: P A
authors.initials: PA
authors.lastname: Megan
mesh_terms: D000465:Algorithms; D002467:Cell Nucleus / Q000032:analysis*; D004273:DNA, Neoplasm / Q000648:ultrastructure*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008214:Lymphocytes / Q000032:analysis; D008853:Microscopy / Q000379:methods*; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000401:mortality; D011003:Ploidies; D016019:Survival Analysis
chemical_list: D004273:DNA, Neoplasm