Temperature-dependence of desensitization induced by acetylcholine and histamine in guinea-pig ileal longitudinal muscle.
1. The effects of temperature on the time course of desensitization induced by acetylcholine and histamine, and on the recovery from desensitization were studied in the longitudinal muscle of the guinea-pig ileum. 2. Self- and cross-desensitization produced by acetylcholine (10(-5) M) occurred rapidly in the first 10 min of exposure to the agonist, with the same time course and the same degree of desensitization over the temperature range of 11 degrees C to 31 degrees C. 3. Self-desensitization produced by histamine (10(-5) M) also occurred rapidly in the first 10 min of exposure to the agonist, and showed great temperature-dependence, especially at 11 degrees C and 21 degrees C, but scarcely occurred at 6 degrees C. 4. Cross-desensitization produced by histamine developed gradually with time and showed a moderate temperature-dependence between 11 degrees C and 31 degrees C, but scarcely occurred at 6 degrees C. 5. The recovery processes from desensitization showed marked temperature-dependence. Recovery was halted completely at 11 degrees C. 6. These studies suggest that acetylcholine-induced desensitization may be attributed to a single non-specific mechanism. Histamine-induced desensitization may be due to at least two mechanisms: it occurs in both a specific and non-specific manner. Each of these desensitizations can be characterized by its unique temperature-dependence.
Horio
S
S
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
Shima
M
M
Ueda
H
H
Ishida
Y
Y
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D005260:Female; D006168:Guinea Pigs; D006207:Half-Life; D006632:Histamine / Q000494:pharmacology*; D007082:Ileum / Q000187:drug effects; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D009130:Muscle, Smooth / Q000187:drug effects*; D013696:Temperature
D006632:Histamine; D000109:Acetylcholine