Plasma and brain kinetics of large neutral amino acids and of striatum monoamines in rats given aspartame.
Two doses (250 and 1000 mg/kg body weight) of aspartame were administered orally to male rats, and plasma and brain phenylalanine and tyrosine kinetic profiles were studied. In both plasma and brain the maximum increase in phenylalanine and tyrosine levels was reached 60 min after treatment. The changes in brain levels of phenylalanine or tyrosine 0, 60, 120 or 180 min after treatment with 1000 mg AMP/kg were directly correlated with the ratio of the plasma concentration of phenylalanine or tyrosine to the overall plasma concentration of the other large neutral amino acids. The time course of monoamine and metabolite concentrations, in the corpora striatum of the brain, was studied after an oral dose of 500 mg phenylalanine/kg. No significant modifications of monoamine levels were found at any of the times studied, up to 5 hr after dosing.
Romano
M
M
Istituto di Ricerche Farmacologiche Mario Negri, Milano, Italy.
Diomede
L
L
Guiso
G
G
Caccia
S
S
Perego
C
C
Salmona
M
M
D000818:Animals; D001218:Aspartame / Q000008:administration & dosage / Q000493:pharmacokinetics*; D015306:Biogenic Monoamines / Q000032:analysis*; D003342:Corpus Striatum / Q000032:analysis*; D004151:Dipeptides / Q000493:pharmacokinetics*; D010649:Phenylalanine / Q000032:analysis* / Q000097:blood; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014443:Tyrosine / Q000032:analysis* / Q000097:blood
D015306:Biogenic Monoamines; D004151:Dipeptides; D014443:Tyrosine; D010649:Phenylalanine; D001218:Aspartame