Correction of the N-terminal sequences of the human plastin isoforms by using anchored polymerase chain reaction: identification of a potential calcium-binding domain.
Plastins are a family of at least three cytoplasmic protein isoforms that are expressed differentially between cells of the hematopoietic lineages and cells of solid tissues. Expression of the L-plastin isoform appears to be restricted to replicating blood cells, and the two T-plastin isoforms appear to be restricted to replicating cells of solid tissues. However, L-plastin is induced in many human solid tumor-derived cells. We used the anchored polymerase chain reaction technique to amplify and clone the missing 5' ends of plastin mRNAs. We found that both plastin isoforms contain a potential calcium binding site near the N terminus.
Lin
C S
CS
California Institute for Medical Research, San Jose 95128.
Aebersold
R H
RH
Leavitt
J
J
D000595:Amino Acid Sequence; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism*; D002147:Calmodulin / Q000235:genetics; D004247:DNA / Q000235:genetics; D014644:Genetic Variation; D006801:Humans; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010750:Phosphoproteins; D016133:Polymerase Chain Reaction; D011485:Protein Binding; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D002147:Calmodulin; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; D015345:Oligonucleotide Probes; D010750:Phosphoproteins; D011506:Proteins; D012333:RNA, Messenger; C026742:plastin; D004247:DNA; D002118:Calcium