title: Antibody to the capsular polysaccharide/adhesin protects rabbits against catheter-related bacteremia due to coagulase-negative staphylococci.
abstract: A rabbit model of catheter-related bacteremia was developed to study immunity to the capsular polysaccharide/adhesin (PS/A) of coagulase-negative staphylococci. Catheters colonized by coagulase-negative staphylococci were inserted into the right jugular vein and attached to a subcutaneous osmotic pump, and blood cultures were obtained over 14 days. Nonimmune rabbits were bacteremic for 6-8 days after infection, hypoglycemic, and hyperlipidemic and had strong immune responses to teichoic acid but not to PS/A. PS/A immunization, but not teichoic acid immunization, reduced the number of bacteremic days by approximately 60%, diminished the hypoglycemia and hyperlipidemia, and ablated the immune responses to teichoic acid. Passive infusion of PS/A-specific polyclonal and monoclonal antibodies using a separate, noninfected catheter-pump combination implanted in the left jugular protected against both bacteremia and hematogenous colonization of this contralateral catheter.
authors.affiliation: Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts.
authors.forename: Y
authors.initials: Y
authors.lastname: Kojima
authors.forename: M
authors.initials: M
authors.lastname: Tojo
authors.forename: D A
authors.initials: DA
authors.lastname: Goldmann
authors.forename: T D
authors.initials: TD
authors.lastname: Tosteson
authors.forename: G B
authors.initials: GB
authors.lastname: Pier
mesh_terms: D000818:Animals; D001774:Blood Chemical Analysis; D001786:Blood Glucose / Q000032:analysis; D002408:Catheters, Indwelling; D004195:Disease Models, Animal; D007116:Immunization, Passive; D015918:Infusion Pumps, Implantable; D008055:Lipids / Q000097:blood; D010587:Phagocytosis; D011135:Polysaccharides, Bacterial / Q000008:administration & dosage / Q000276:immunology*; D011817:Rabbits; D018805:Sepsis / Q000276:immunology* / Q000517:prevention & control; D013203:Staphylococcal Infections / Q000276:immunology* / Q000517:prevention & control; D013212:Staphylococcus epidermidis / Q000276:immunology*; D013682:Teichoic Acids / Q000276:immunology
chemical_list: D001786:Blood Glucose; D008055:Lipids; D011135:Polysaccharides, Bacterial; D013682:Teichoic Acids