Suppression of interleukin-2 mediated T-lymphocyte blastogenesis by bovine uterine secretions.
Uterine luminal protein (ULP) secretions collected on day 17 of bovine pregnancy contain high (greater than or equal to 248 kDa)- and low (7, 21.0, and greater than or equal to 72 kDa)-molecular weight (Mr) components that suppressed incorporation of thymidine into both phytohemagglutinin (PHA)-stimulated and interleukin-2 (IL-2)-stimulated bovine lymphocytes. The pattern of suppressor activity for ULP was similar for both PHA and IL-2 cultures. For IL-2-treated lymphocytes (2 X 10(4)/culture well), mean percentage of control (no test protein) values for 8 and 32 micrograms/ml of high Mr ULP were 94.6% and 4.5%, respectively; whereas, mean values for 8 and 64 micrograms/ml of combined low Mr ULP components were 51.2% and 5.5%, respectively. The data indicate that specific bovine ULP components may locally affect T-lymphocyte function by altering some facet of the IL-2 activation system.
Segerson
E C
EC
Department of Animal Science, North Carolina A&T State University, Greensboro 27411.
Gunsett
F C
FC
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D002850:Chromatography, Gel; D005260:Female; D006023:Glycoproteins; D007376:Interleukin-2 / Q000037:antagonists & inhibitors*; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000276:immunology*; D011506:Proteins / Q000302:isolation & purification / Q000502:physiology*; D011994:Recombinant Proteins; D015843:Serpins; D013601:T-Lymphocytes / Q000276:immunology*
D006023:Glycoproteins; D007376:Interleukin-2; D010835:Phytohemagglutinins; D011506:Proteins; D011994:Recombinant Proteins; D015843:Serpins; C029708:uterine luminal fluid proteins