[Reaction between monoclonal antibodies to porcine zona pellucida and human and porcine ovary in vitro].
Reaction between monoclonal antibodies to porcine zona pellucida (LPD8, LPC4) and human and porcine ovaries was studied by ABC immunohistochemistry technique in vitro. When slices of human and porcine ovaries were stained with ABC, not only the zona pellucida but also the ovum cytoplasm was stained. Other regions of the ovary, such as granulosa cell, theca cell and connective tissue, could not be stained. These results suggest that the monoclonal antibodies to porcine zona pellucida probably have no direct effect on the endocrine function of ovary, but they may combine with ovum cytoplasm and inhibit follicular growth, so that the number of follicles reduces and endocrine function of the ovary decreases. We do not think that the prospects of continual study on LPD8, LPC4 are good.
Wen
J
J
Liaoning Provincial Institute of Family Planning Research.
Jiang
Q
Q
Guan
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003593:Cytoplasm / Q000276:immunology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D010053:Ovary / Q000276:immunology*; D010063:Ovum / Q000276:immunology*; D013552:Swine; D015044:Zona Pellucida / Q000276:immunology*
D000911:Antibodies, Monoclonal