Stimulation of phospholipase C-gamma 1 membrane association by epidermal growth factor.
Epidermal growth factor (EGF) treatment of A-431 epidermoid carcinoma cells elicited a redistribution of phospholipase C-gamma 1 (PLC-gamma 1) from a predominantly cytosolic localization to membrane fractions. The temporal coincidence of this redistribution with EGF stimulation of inositol phosphate formation and EGF increased phosphorylation of PLC-gamma 1 suggests that the membrane association of PLC-gamma 1 is a significant event in second messenger transduction.
Todderud
G
G
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232-0146.
Wahl
M I
MI
Rhee
S G
SG
Carpenter
G
G
D002294:Carcinoma, Squamous Cell; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D007700:Kinetics; D010748:Phosphopeptides / Q000302:isolation & purification; D011485:Protein Binding; D014357:Trypsin; D010738:Type C Phospholipases / Q000378:metabolism*
D007527:Isoenzymes; D010748:Phosphopeptides; D004815:Epidermal Growth Factor; D010738:Type C Phospholipases; D014357:Trypsin