Estimation of the analgesic muscle relaxants chlorzoxazone and diazepam in human plasma by reversed-phase liquid chromatography.
A rapid and sensitive method of extraction of human plasma containing acetaminophen, chlorzoxazone, oxyphenbutazone and diazepam along with their active metabolites was developed. The plasma samples were extracted by a solid-phase extraction procedure with theophylline as an internal standard for accurate quantitation. The reversed-phase liquid chromatographic method provided a linear ultraviolet detector response in the range 100.0-700.0 ng/ml. Recoveries greater than 95% were achieved from human plasma samples. Limits of detection of 100, 200, 150 and 250 ng/ml for acetaminophen, chlorzoxazone, oxyphenbutazone and diazepam, respectively, were obtained.
Gaitonde
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Applications and Methods Development Laboratory, Anamed Instruments (Pvt.) Ltd., New Bombay, India.
Pathak
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D000082:Acetaminophen / Q000097:blood; D000700:Analgesics / Q000097:blood*; D001583:Benzoxazoles / Q000097:blood*; D002753:Chlorzoxazone / Q000097:blood*; D002845:Chromatography / Q000379:methods; D003975:Diazepam / Q000097:blood*; D006801:Humans; D009125:Muscle Relaxants, Central / Q000097:blood*; D010113:Oxyphenbutazone / Q000097:blood; D013806:Theophylline / Q000097:blood
D000700:Analgesics; D001583:Benzoxazoles; D009125:Muscle Relaxants, Central; D000082:Acetaminophen; D013806:Theophylline; D002753:Chlorzoxazone; D010113:Oxyphenbutazone; D003975:Diazepam