title: Human gingival Langerhans cells stimulate allogeneic lymphocytes: requirement for MHC class II antigens.
abstract: Langerhans cells (LC) are antigen-presenting cells which express high levels of Class II MHC antigens on their plasma membranes. While the expression of these antigens on gingival LC has been documented, their functional significance is unclear. In this study, the mixed epithelial cell-lymphocyte culture reaction (MECLR) between stimulator cells (LC) and allogenic lymphocytes was used as an in vitro model for investigating the role of the MHC Class II antigens HLA-DR, -DQ, and -DP in alloantigen presentation by gingival LC. In epithelial cell suspensions prepared from human gingiva, MHC Class II antigen expression (HLA-DR, -DP, -DQ) was confined to CD1a-positive LC. Depletion of Class II antigen-bearing LC from epithelial cells using monoclonal antibodies (L243, B7/21, and SK10) and complement inhibited the ability of epithelial cells to stimulate proliferation in the MECLR. Pre-treatment of epithelial cell suspensions with the same monoclonal antibodies suppressed proliferation in the MECLR, as did direct addition of these antibodies to co-cultures of epithelial cells and lymphocytes. These results indicate that HLA-DQ and -DP, together with DR antigens on gingival LC, are involved in LC-lymphocyte interactions. Since LC are potent antigen presenting cells, alterations in the expression of MHC Class II antigens on the surface of these cells will influence their ability to stimulate lymphocytes during the initiation of the cellular immune response to the accumulation of dental plaque.
authors.affiliation: Department of Social and Preventive Dentistry, University of Queensland Dental School, Brisbane Australia.
authors.forename: L J
authors.initials: LJ
authors.lastname: Walsh
authors.forename: G J
authors.initials: GJ
authors.lastname: Seymour
authors.forename: R N
authors.initials: RN
authors.lastname: Powell
mesh_terms: D000911:Antibodies, Monoclonal; D002455:Cell Division; D002478:Cells, Cultured; D004847:Epithelial Cells; D005434:Flow Cytometry; D005881:Gingiva / Q000166:cytology*; D006680:HLA Antigens / Q000032:analysis / Q000502:physiology*; D006682:HLA-DP Antigens / Q000032:analysis / Q000502:physiology; D006683:HLA-DQ Antigens / Q000032:analysis / Q000502:physiology; D006684:HLA-DR Antigens / Q000032:analysis / Q000502:physiology; D006801:Humans; D007801:Langerhans Cells / Q000276:immunology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D009000:Monocytes / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D006680:HLA Antigens; D006682:HLA-DP Antigens; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens