Viral determinants of human immunodeficiency virus type 1 T-cell or macrophage tropism, cytopathogenicity, and CD4 antigen modulation.
The genome of the human immunodeficiency virus type 1 (HIV-1) is highly heterogeneous. Some of this genomic variability is reflected in the biologic and serologic differences observed among various strains of HIV-1. To map the viral determinants that correlate with pathogenicity of the virus, recombinant viruses were generated between biologically active molecular clones of HIV-1 strains that show differences in T-cell or macrophage tropism, cytopathogenicity, CD4 antigen modulation, and susceptibility to serum neutralization. The results of these studies indicate that the envelope region contains the major determinants of these viral features. Further studies with sequence exchanges within this region should help identify specific domains that contribute to HIV pathogenesis.
Cheng-Mayer
C
C
Cancer Research Institute, University of California, San Francisco 94143.
Quiroga
M
M
Tung
J W
JW
Dina
D
D
Levy
J A
JA
D000595:Amino Acid Sequence; D015703:Antigens, CD / Q000276:immunology; D001483:Base Sequence; D015139:Blotting, Southern; D015704:CD4 Antigens / Q000276:immunology*; D002460:Cell Line; D002472:Cell Transformation, Viral; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005814:Genes, Viral; D015497:HIV-1 / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008264:Macrophages / Q000276:immunology / Q000382:microbiology*; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013601:T-Lymphocytes / Q000276:immunology / Q000382:microbiology*; D014759:Viral Envelope Proteins / Q000235:genetics
D015703:Antigens, CD; D015704:CD4 Antigens; D004279:DNA, Viral; D014759:Viral Envelope Proteins