Dependence between 5'-nucleotidase activity and phosphatidylcholine content in pig adipocyte plasma membrane. Absence of relationship with membrane physical state.
1. 5'-Nucleotidase activity has been examined in pig adipocyte plasma membrane, in two different adipose sites. 2. Membrane fluidity has been investigated using 1,6-diphenylhexatriene (DPH). 3. In this work, evidence was found for a tissue specificity of 5'-nucleotidase (E.C. 3.1.3.5) activity. 4. The enzyme activity was highly correlated to the membrane phosphatidylcholine content. 5. The latter parameters remained unmodified when large alterations in membrane composition and physical state were produced by means of the dietary n-6 PUFA content manipulations.
Nicolas
C
C
Laboratoire de Nutrition et Sécurité Alimentaire, INRA-CRJ, Jouy-en-Josas, France.
Demarne
Y
Y
Lecourtier
M J
MJ
Lhuillery
C
C
D015720:5'-Nucleotidase / Q000378:metabolism*; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage; D066298:In Vitro Techniques; D008297:Male; D008560:Membrane Fluidity; D010713:Phosphatidylcholines / Q000378:metabolism*; D013552:Swine
D004042:Dietary Fats, Unsaturated; D010713:Phosphatidylcholines; D015720:5'-Nucleotidase