Suppression of spontaneous generalized non-convulsive seizures in the rat by microinjection of GABA antagonists into the superior colliculus.
Intranigral injections of GABA agonists suppress spontaneous and chemically induced generalized non-convulsive seizures in the rat. In order to examine whether the GABAergic nigrotectal pathway could be involved in this suppression, bilateral injections of GABA antagonists were performed in the superior colliculus of rats with spontaneous generalized non-convulsive seizures. Bilateral microinjections into this structure of the GABA antagonists picrotoxin (20 and 40 ng/side) and bicuculline methiodide (5 ng/side) suppressed spike-and-wave discharges for 40 min and 20 min post injection, respectively. Unilateral injections of picrotoxin (40 ng) into the superior colliculus as well as bilateral injections of a GABA agonist (muscimol; 80 ng) did not induce significant modifications. These results show that blockade of the GABAergic transmission at the level of the superior colliculus results in a suppression of generalized non-convulsive seizures. These data support the hypothesis that the suppressive effect of intranigral injections of GABA agonists over generalized non-convulsive seizures involves, at least in part, the nigrotectal GABAergic pathway.
Depaulis
A
A
Département de Neurophysiologie et Biologie des Comportements, U.44 INSERM/Centre de Neurochimie du CNRS, Strasbourg, France.
Liu
Z
Z
Vergnes
M
M
Marescaux
C
C
Micheletti
G
G
Warter
J M
JM
D000818:Animals; D001640:Bicuculline / Q000494:pharmacology*; D018756:GABA Antagonists; D008297:Male; D008845:Microinjections; D010852:Picrotoxin / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012640:Seizures / Q000378:metabolism / Q000503:physiopathology*; D013477:Superior Colliculi / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism / Q000502:physiology*
D001640:Bicuculline; D018756:GABA Antagonists; D010852:Picrotoxin; D005680:gamma-Aminobutyric Acid