title: Evidence in vitro for an enzymatic synthesis of phosphocitrate.
abstract: A biological synthesis of phosphocitrate is described from precursors, citrate and adenosinetriphosphate reacting in the presence of rat liver homogenate. Identity of the newly formed product was examined by enzymatic digestion of reactions mixtures, HPLC chromatography and 1H-NMR spectra. Authenticity of product was established by comparison to chemically synthesized phosphocitrate. Recognition of the existence of a biologically synthetic pathway adds credence to the known presence of phosphocitrate in mitochondria and a postulated role to control calcium phosphate deposition in that organelle.
authors.affiliation: Dipartimento di Biochimica, Università degli Studi di Trieste, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Moro
authors.forename: N
authors.initials: N
authors.lastname: Stagni
authors.forename: E
authors.initials: E
authors.lastname: Luxich
authors.forename: J D
authors.initials: JD
authors.lastname: Sallis
authors.forename: B
authors.initials: B
authors.lastname: de Bernard
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002951:Citrates / Q000378:metabolism*; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D051381:Rats; D014020:Tissue Extracts
chemical_list: D002951:Citrates; D014020:Tissue Extracts; C014148:phosphocitrate