Autoradiographic localization of 2-[125I]iodomelatonin binding sites in the brains of C3H/HeN and C57BL/6J strains of mice.
The present study uses in vitro autoradiography to localize 2-[125I]iodomelatonin binding sites in the brains of two strains of mice, the C3H/HeN and the C57BL/6J, which have been shown to exhibit differences in pineal melatonin content. We found a differential pattern of distribution of 2-[125I]iodomelatonin binding sites between the two strains with the suprachiasmatic nucleus, paraventricular nucleus of the thalamus, and the median eminence/pars tuberalis regions labelled in both strains. These studies should help to interpret behavioral changes due to activation of melatonin receptors in these strains of mice.
Siuciak
J A
JA
Northwestern University Medical School, Department of Pharmacology, Chicago, IL 60611.
Fang
J M
JM
Dubocovich
M L
ML
D000818:Animals; D001345:Autoradiography; D001665:Binding Sites; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism; D007457:Iodine Radioisotopes; D008297:Male; D008550:Melatonin / Q000031:analogs & derivatives* / Q000378:metabolism / Q000493:pharmacokinetics; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D013045:Species Specificity
D007457:Iodine Radioisotopes; C044556:2-iodomelatonin; D008550:Melatonin