Effects of phenylhydrazine hydrochloride on energy metabolism in rabbit erythrocytes and reticulocytes.
The effects of 1, 5 and 20 mmol/l phenylhydrazine hydrochloride on energy metabolism of rabbit erythrocyte and reticulocyte were studied. Significant depression of glycolysis, accompanied by loss of adenine nucleotides, mainly due to an extensive decline of ATP, was found in erythrocytes. Energy metabolism of reticulocytes appears to be more sensitive to deleterious effects of this drug. The declines of ATP and sum of all adenine nucleotides, as well as the accumulation of hypoxanthine were twofold in reticulocyte-rich red cell suspensions compared with suspensions of mature erythrocytes. It can be concluded that these changes are the consequence of lower energy production due to phenylhydrazine hydrochloride-induced inhibition of oxidative phosphorylation.
Zivkovic
R V
RV
Institute of Biochemistry, Faculty of Medicine, University Belgrade, Yugoslavia.
Kostic
M M
MM
Siems
W
W
Werner
A
A
Mojsilovic
L P
LP
Gerber
G
G
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D004734:Energy Metabolism / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D010085:Oxidative Phosphorylation; D010659:Phenylhydrazines / Q000494:pharmacology*; D011817:Rabbits; D012156:Reticulocytes / Q000187:drug effects / Q000378:metabolism*
D000227:Adenine Nucleotides; D007773:Lactates; D010659:Phenylhydrazines; C030299:phenylhydrazine; D005947:Glucose