Lack of DNA single-strand breaks in rat liver cells exposed to 4-acetylaminofluorene, in vivo and in vitro.
The induction of primary DNA damage by the non-carcinogen 4-AAF was reinvestigated in liver cells by comparison with the carcinogen 2-AAF. DNA alkaline elution showed the appearance of single-strand breaks in total liver DNA of rats 4 h after gavage with 200 mg/kg of 4-AAF. The decrease in hepatocyte viability and yield observed in these livers after collagenase perfusion indicated a cytotoxic effect of 4-AAF treatment. Viable hepatocytes isolated from 4-AAF-treated rats as well as hepatocytes from normal rats treated with 4-AAF in vitro did not present DNA single-strand breaks.
Nizard
C
C
Institut de Recherches Scientifiques sur le Cancer, Villejuif, France.
Decloître
F
F
D015073:2-Acetylaminofluorene / Q000494:pharmacology / Q000633:toxicity* / Q000031:analogs & derivatives; D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D002470:Cell Survival; D004277:DNA, Single-Stranded / Q000187:drug effects*; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D008297:Male; D051381:Rats
D002273:Carcinogens; D004277:DNA, Single-Stranded; D015073:2-Acetylaminofluorene; D007770:L-Lactate Dehydrogenase; C028676:4-acetylaminofluorene