title: NMR studies of the lipid metabolism of T47D human breast cancer spheroids.
abstract: The in vivo 31P NMR spectrum of T47D human breast cancer cells grown as spheroids shows changes in the phosphomonoester lipid precursors as a function of spheroid size. The ratio of phosphorylethanolamine (PE) to phosphorylcholine (PC) was 1.0 +/- 0.3 for 3-day-old, 150 microns spheroids. This ratio increased to 2.4 +/- 0.4 for spheroids 7 days and older and which were at least 300 microns in diameter. To investigate the phosphatidylethanolamine to phosphatidylcholine (PdylE/PdylC) ratio in the membranes, chloroform/methanol extracts of spheroids were performed. The 31P spectrum of these extracts showed no change with spheroid size, namely the PdylE/PdylC ratio was 0.5 +/- 0.06 for spheroids of all ages suggesting that membrane composition is strongly regulated at the precursor level.
authors.affiliation: Isotope Department, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: S M
authors.initials: SM
authors.lastname: Ronen
authors.forename: A
authors.initials: A
authors.lastname: Stier
authors.forename: H
authors.initials: H
authors.lastname: Degani
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D008563:Membrane Lipids / Q000378:metabolism; D009940:Organoids; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D013997:Time Factors; D014407:Tumor Cells, Cultured
chemical_list: D008563:Membrane Lipids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids