Mycoplasma can enhance HIV replication in vitro: a possible cofactor responsible for the progression of AIDS.
Mycoplasma membrane protein (MMP) augmented HIV production from MOLT-4 cells chronically infected by HIV. A nearly 3-fold increase of HIV p24 antigen was detected in MMP-treated culture as compared to control culture at 100 micrograms/ml. MMP also augmented HIV production in different T cells chronically infected by HIV-1 and ARV-1. Kinetic experiment showed that HIV production was maximally elevated 24 hr after exposure to MMP. Similarly, MMP also augmented HIV-induced cell fusion and virus production in coculture. Hybridization experiment revealed that this augmentation was due to enhancement of HIV transcription.
Chowdhury
I H
IH
Department of Virology and Parasitology, Yamaguchi University School of Medicine, Japan.
Munakata
T
T
Koyanagi
Y
Y
Kobayashi
S
S
Arai
S
S
Yamamoto
N
N
D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D015488:HIV Antigens / Q000032:analysis; D015497:HIV-1 / Q000276:immunology / Q000502:physiology*; D006801:Humans; D008565:Membrane Proteins / Q000494:pharmacology*; D009174:Mycoplasma / Q000378:metabolism*; D013601:T-Lymphocytes; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology; D014779:Virus Replication / Q000187:drug effects*
D000911:Antibodies, Monoclonal; D015488:HIV Antigens; D008565:Membrane Proteins; D014409:Tumor Necrosis Factor-alpha