title: Localization of aldolase C mRNA in brain cells.
abstract: The expression of aldolase C and aldolase A mRNA was assessed by Northern blot hybridization using RNAs purified from cultured rat and mouse brain neurons and astroglial cells. Neurons were found to contain about 4-fold more aldolase C mRNA and about twice as much aldolase A mRNA than astroglia. Analysis of the cellular localization of aldolase C mRNA by in situ hybridization to brain slices showed a predominantly neuronal labeling with an irregular distribution. A strong signal was observed in Purkinje cell somata and a weaker signal in subpopulations of neurons in cerebral cortex, striatum, hippocampus, hypothalamic nuclei and primary olfactory cortex.
authors.affiliation: INSERM U129, Paris, France.
authors.forename: T
authors.initials: T
authors.lastname: Popovici
authors.forename: Y
authors.initials: Y
authors.lastname: Berwald-Netter
authors.forename: M
authors.initials: M
authors.lastname: Vibert
authors.forename: A
authors.initials: A
authors.lastname: Kahn
authors.forename: H
authors.initials: H
authors.lastname: Skala
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000201:enzymology; D015152:Blotting, Northern; D001921:Brain / Q000166:cytology / Q000201:enzymology*; D001931:Brain Mapping; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D009474:Neurons / Q000201:enzymology; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D012333:RNA, Messenger; D005634:Fructose-Bisphosphate Aldolase