[The determination of total coliform and fecal coliform bacteria for quality control of bathing waters using the EC guideline 76/160 most probable number and BRILA-MUG broth].
In the monitoring of bathing waters according to EC Guideline 76/160 EWG, total coliform and faecal coliform bacteria have to be regularly determined by a quantitative method. This requirement can be fulfilled with sufficient reliability by the MPN method. Therefore, a procedure had to be developed which on the one hand is practical and not laborious, and on the other hand warrants a sufficiently specific, quantitative bacteriological diagnostic. The commercially available BRILA-MUG-broth appeared suitable for this purpose as it allows a direct determination of total as well as faecal coliform bacteria in the same assay. By this method, total coliform bacteria are recognized by gas production from lactose whereas faecal coliform bacteria additionally produce indole and liberate fluorochrome from MUG. Theoretical considerations and practical experiences showed that the rate of false-positive as well as false-negative results was within a range of 10% if compared with reactions described in the literature. Some factors which may influence the results are discussed.
Müller
H E
HE
Staatliches Medizinaluntersuchungsamt Braunschweig.
Aleksic
S
S
Bockemühl
J
J
Havemeister
G
G
Heinemeyer
E A
EA
Von Pritzbuer
E
E
D015169:Colony Count, Microbial; D003470:Culture Media; D004755:Enterobacteriaceae / Q000254:growth & development*; D005243:Feces / Q000382:microbiology; D013550:Swimming; D014871:Water Microbiology
D003470:Culture Media
Die Bestimmung der gesamtcoliformen und faekalcoliformen Bakterien zur Qualitätsüberwachung von Badegewässern nach der EG-Richtlinie 76/160 EWG mit Hilfe der BRILA-MUG-Bouillon.