The antifertility effect of passive immunization of mice against progesterone is influenced by genetic factors other than H-2 haplotype.
The anti-fertility effect of passive immunization against progesterone is influenced by genotype in mice. In order to quantify this finding we determined the effective dose that blocks pregnancy in 50% of treated mice (ED50) for two different anti-progesterone monoclonal antibodies (DB3 and 11/32) in four different strains of mice (BALB/cJ, CBA/Ca, Tuck's no. 1 and F1C). Efficacy was greatest in the two inbred strains (BALB/cJ and CBA/Ca) with ED50 values of 0.46-1.4 nmol. The F1C hybrid mice were more resistant to antibody treatment (ED50 2.2-3.0 nmol), while the outbred Tuck's no. 1 strain required much higher doses (ED50 7.0-8.2 nmol). There were no intrastrain differences between the two monoclonal antibodies. We have examined the possible role of the H-2 haplotype on antibody efficacy in different strains and crosses. The antibody was highly effective in blocking implantation in three congenic BALB strains of different H-2 haplotype, in another inbred strain CBA/Ca, and in reciprocal BALB/cJ x CBA/Ca crosses. The F1 hybrid crosses were somewhat resistant, but the C57BL/10Sn and B10.BR congenic strains were most resistant to treatment. The results show that the pregnancy-blocking effect of the anti-progesterone antibody was not influenced by the H-2 haplotype, but rather by background genes.
Nowak
R A
RA
AFRC Institute of Animal Physiology and Genetics Research, Cambridge Research Station, Babraham.
Wang
M W
MW
Heap
R B
RB
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage; D004305:Dose-Response Relationship, Drug; D005260:Female; D005298:Fertility / Q000276:immunology*; D005838:Genotype; D006239:Haplotypes / Q000276:immunology; D007116:Immunization, Passive; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000187:drug effects* / Q000276:immunology; D011374:Progesterone / Q000276:immunology*
D000911:Antibodies, Monoclonal; D011374:Progesterone