title: The effects of feeding a saturated fat-rich diet on enzymes of cholesterol metabolism in the liver, intestine and aorta of the hamster.
abstract: The effects of two dietary treatments on parameters of cholesterol metabolism were studied. Hamsters were maintained on diets containing 2% (w/w) cholesterol or 20% (w/w) hydrogenated coconut oil for 4 weeks. Both diets induced a hypercholesterolaemia. The effects of the two treatments on hepatic and intestinal acyl-CoA:cholesterol acyltransferase activity and 3-hydroxy-3-methylglutaryl-CoA reductase activity were measured. As expected, cholesterol feeding stimulated cholesterol esterification and inhibited cholesterol synthesis. Saturated fat-feeding had no effect on cholesterol synthesis but markedly inhibited cholesterol esterification in both liver and intestine. The diet-induced hypercholesterolaemia was strongly correlated with an increase in acyl-CoA:cholesterol acyltransferase activity in the activity. In contrast, the hypercholesterolaemia induced by feeding either of the two diets tended to increase aortic uptake of cholesterol and hence acyl-CoA:cholesterol acyltransferase activity. We suggest that the changes in cholesterol esterification correlate well with the expected flux of cholesterol into each tissue.
authors.affiliation: Department of Cellular Pharmacology, Smith Kline & French Research Ltd., Welwyn, U.K.
authors.forename: B
authors.initials: B
authors.lastname: Jackson
authors.forename: A N
authors.initials: AN
authors.lastname: Gee
authors.forename: M
authors.initials: M
authors.lastname: Martinez-Cayuela
authors.forename: K E
authors.initials: KE
authors.lastname: Suckling
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects / Q000201:enzymology*; D002784:Cholesterol / Q000378:metabolism*; D002788:Cholesterol Esters / Q000378:metabolism; D002791:Cholesterol, Dietary / Q000494:pharmacology*; D000074263:Coconut Oil; D003059:Cocos; D006224:Cricetinae; D004041:Dietary Fats / Q000494:pharmacology*; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism*; D007082:Ileum / Q000187:drug effects / Q000201:enzymology*; D007700:Kinetics; D008074:Lipoproteins / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008647:Mesocricetus; D009130:Muscle, Smooth / Q000187:drug effects / Q000201:enzymology*; D010938:Plant Oils; D012016:Reference Values; D002785:Sterol O-Acyltransferase / Q000378:metabolism*
chemical_list: D002788:Cholesterol Esters; D002791:Cholesterol, Dietary; D004041:Dietary Fats; D008074:Lipoproteins; D010938:Plant Oils; D002784:Cholesterol; D006903:Hydroxymethylglutaryl CoA Reductases; D002785:Sterol O-Acyltransferase; D000074263:Coconut Oil