Analysis of xanthene dyes by reversed-phase high-performance liquid chromatography on a polymeric column followed by characterization with a diode array detector.
A high-performance liquid chromatographic method on a polymeric column was developed for the analysis of xanthene dyes. The rigid polystyrene-divinylbenzene column was connected to a photodiode array detector to verify the identity and the purity of the dyes. For eosin Y a within-day precision of 1-2% was obtained, and on a day-to-day basis the coefficient of variation was 4.2%. The purity of commercial xanthene dyes was investigated, and the results show the divergence between the actual dye contents and the dye contents indicated on the label.
Van Liedekerke
B M
BM
Laboratoria voor Medische Biochemie en voor Klinische Analyse, Ghent, Belgium.
De Leenheer
A P
AP
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004396:Coloring Agents / Q000032:analysis* / Q000302:isolation & purification; D015394:Molecular Structure; D013329:Structure-Activity Relationship; D014966:Xanthenes / Q000032:analysis* / Q000302:isolation & purification
D004396:Coloring Agents; D014966:Xanthenes