Detection of the exercise-associated changes in serum proteins by two-dimensional electrophoresis under non-denaturing conditions.
The changes in serum proteins caused by physical exercise (10-km run) were examined using two-dimensional electrophoresis under non-denaturing conditions. Two specific proteins were found to increase remarkably in amount. Both proteins had a slightly higher molecular mass than albumin, which suggests that they are albumin-bound with large amounts of sugars or lipids. However, these proteins were not adsorbed on an anti-albumin affinity column, and they were not stained by either periodic acid-Schiff base or Sudan Black. The molecular mass was determined to be 25,000 by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The changes of the specific proteins in competitors in a triathlon race were also examined.
Hiro
T
T
Laboratory of Physical Education, School of Dental Medicine, Tsurumi University, Yokohama, Japan.
Kadofuku
T
T
Makino
Y
Y
Sato
T
T
D000328:Adult; D001798:Blood Proteins / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional; D015444:Exercise; D005260:Female; D006801:Humans; D008297:Male; D011489:Protein Denaturation; D013177:Sports
D001798:Blood Proteins