Immunoelectron microscopical labelling of a glycolipid in the envelopes of brain cell-derived budding viruses, Semliki Forest, influenza and measles, using a monoclonal antibody directed chiefly against galactocerebroside resulting from Semliki Forest virus infection.
Neurotropic RNA budding viruses such as Semliki Forest virus (SFV), influenza and measles were each grown in identical mouse brain cell cultures. Positive immunoelectron microscopical labelling with gold was seen in the envelope of these viruses using an anti-SFV derived glycolipid monoclonal antibody (MAb), 373 shown to be directed chiefly against galactocerebroside. The results indicate that each enveloped virus grown from the same cell type contains the same glycolipid in its envelope. The presence of common glycolipids derived from the host cell in the envelopes of various enveloped budding viruses may play a significant role in the pathogenesis of virus induced, immune mediated CNS autoimmunity and demyelination, particularly in multiple sclerosis (MS).
Pathak
S
S
Department of Neurovirology, United Medical School of Guy's Hospital, London, U.K.
Illavia
S J
SJ
Khalili-Shirazi
A
A
Webb
H E
HE
D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D002554:Cerebrosides / Q000378:metabolism*; D005699:Galactosylceramides / Q000378:metabolism*; D006017:Glycolipids / Q000378:metabolism*; D007150:Immunohistochemistry; D008459:Measles virus / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron; D009975:Orthomyxoviridae / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D012672:Semliki forest virus / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D014759:Viral Envelope Proteins / Q000378:metabolism*; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D002554:Cerebrosides; D005699:Galactosylceramides; D006017:Glycolipids; D014759:Viral Envelope Proteins