title: Freezing rabbit semen by the use of BF5 diluent.
abstract: Three experiments were carried out to find the optimal concentration of DMSO and glycerol in BF5 diluent for freezing rabbit spermatozoa. Semen was diluted 1:1 with diluent A (BF5 + DMSO) at 25 degrees C and diluted further 1:1 with diluent B (diluent A + glycerol) after cooling down to 5 degrees C. Diluted semen was frozen immediately and stored in liquid nitrogen. Maximum percentages for motility and normal acrosomes were obtained in the presence of 12% DMSO (as expressed in diluent A) and 3% glycerol (final concentration) after thawing.
authors.affiliation: AFRC, Institute of Animal Physiology, Cambridge, UK.
authors.forename: K
authors.initials: K
authors.lastname: Bamba
authors.forename: C E
authors.initials: CE
authors.lastname: Adams
mesh_terms: D000177:Acrosome / Q000187:drug effects; D000818:Animals; D015925:Cryopreservation; D003982:Dibekacin / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004338:Drug Combinations / Q000494:pharmacology; D005260:Female; D005990:Glycerol / Q000494:pharmacology; D008297:Male; D011817:Rabbits; D012662:Semen Preservation; D013081:Sperm Motility / Q000187:drug effects; D013451:Sulfonic Acids / Q000494:pharmacology; D014325:Tromethamine / Q000494:pharmacology
chemical_list: C064927:BF5 diluent; D004338:Drug Combinations; D013451:Sulfonic Acids; D014325:Tromethamine; D003982:Dibekacin; D005990:Glycerol; D004121:Dimethyl Sulfoxide