Serum PAF acetylhydrolase increases during neonatal maturation.
Acetylhydrolase is an acid-labile, 43 kd protein that catalyzes the degradation of platelet activating factor (PAF), a potent phospholipid inflammatory mediator, to its biologically inactive metabolite lysoPAF. PAF has a short half-life, thus acetylhydrolase plays an important role in its regulation. Since previous work suggests that PAF may be involved in certain neonatal diseases such as necrotizing enterocolitis, we studied the effect of age on acetylhydrolase activity. Serum acetylhydrolase activity was quantified using radio-labelled PAF and measuring reaction products. Serum samples were obtained prospectively from 70 subjects ranging in age from 4 hr to 48 yr. Acetylhydrolase activity was lower for newborns (less than 3 wk) than all other age ranges (8.2 +/- 1.4 nmole/ml/min vs 30.0 +/- 1.6 nmole/ml/min, p less than .01). Furthermore, enzyme activity increased linearly with respect to the natural logarithm of age from 0 days to 6 weeks (r = 0.65, p less than .001). By 6 weeks of life acetylhydrolase activity approached values of older children and adults. Newborn acetylhydrolase activity was similar between term and preterm infants (8.6 +/- 1.9 nmole/ml/min vs 7.2 +/- 2.4 nmole/ml/min, p = NS). We conclude that acetylhydrolase activity is low in human neonates and increases during the first 6 weeks of life. These results suggest that newborn infants may be at increased risk for pathophysiologic processes mediated by PAF.
Caplan
M
M
Department of Pediatrics, Evanston Hospital, IL 60201.
Hsueh
W
W
Kelly
A
A
Donovan
M
M
D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase; D000328:Adult; D000375:Aging / Q000097:blood*; D002648:Child; D006330:Heart Defects, Congenital / Q000097:blood / Q000201:enzymology; D006801:Humans; D006933:Hyperbilirubinemia, Hereditary / Q000097:blood / Q000201:enzymology; D007223:Infant; D007231:Infant, Newborn; D007234:Infant, Premature; D010547:Persistent Fetal Circulation Syndrome / Q000097:blood / Q000201:enzymology; D010740:Phospholipases / Q000097:blood*; D010741:Phospholipases A / Q000097:blood*; D012016:Reference Values; D012044:Regression Analysis; D012127:Respiratory Distress Syndrome, Newborn / Q000097:blood / Q000201:enzymology
D010740:Phospholipases; D010741:Phospholipases A; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase