Gangliosides in the human glioma cell line U-118 MG grown in culture or as xenografts in nude rats.
This study was undertaken to characterize gangliosides in the human glioma cell line U-118 MG. The cell line was grown both in cell culture and as xenografts in nude rats. A common finding in both culture and xenograft cells was the high proportion of the lactoseries ganglioside 3'-LM1, approximately one third of the total ganglioside sialic acid. Otherwise, there were marked differences between the two cell sources. The cells grown in culture had a more simple ganglioside pattern than those grown in xenografts. In the latter instance, more complex gangliosides of the lactoseries, including 3'8'-LD1, sialyllactonorhexaosylceramide and a branched structure with two terminal NeuAc alpha 2-3Gal beta 1- 4GlcNAc chains, and the gangliotetraose series were found. Another marked difference involved GM2, which in the cultured cells was a major fraction, indicating that the synthesis of the gangliotetraose series gangliosides in the former stopped at the level of GM2. These results show that the ganglioside composition of a glioma cell line is strongly influenced by environmental factors.
Fredman
P
P
Department of Psychiatry and Neurochemistry, Gothenburg University, St. Jörgen Hospital, Hisings Backa, Sweden.
Månsson
J E
JE
Bigner
S H
SH
Wikstrand
C J
CJ
Bigner
D D
DD
Svennerholm
L
L
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D005732:Gangliosides / Q000032:analysis*; D005910:Glioma / Q000378:metabolism*; D006801:Humans; D009368:Neoplasm Transplantation; D051381:Rats; D012794:Sialic Acids / Q000032:analysis; D014407:Tumor Cells, Cultured / Q000032:analysis
D005732:Gangliosides; D012794:Sialic Acids