A non-curved chicken lysozyme 5' matrix attachment site is 3' followed by a strongly curved DNA sequence.
Matrix attachment regions (MARs) partition the genome into functional and structural loop-domains. Here, we determined the relative matrix affinity of cloned fragments of the chicken lysozyme 5' MAR. We show that this region contains a non-curved high-affinity binding site, which is 3' followed by a strongly curved DNA sequence that exhibits weak matrix binding. DNA curvature is not a physical property required for strong matrix binding. Possible biological functions of this sequence arrangement, particularly of the strongly curved DNA, are discussed.
von Kries
J P
JP
Institut für Physiologische Chemie, Universitäts-Krankenhaus Eppendorf, Hamburg, FRG.
Phi-Van
L
L
Diekmann
S
S
Strätling
W H
WH
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002645:Chickens; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000378:metabolism; D005260:Female; D008969:Molecular Sequence Data; D009113:Muramidase / Q000378:metabolism*; D015530:Nuclear Matrix / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D015183:Restriction Mapping
D004247:DNA; D009113:Muramidase