title: The importance of mast cells for the neutrophil influx in immune complex-induced peritonitis in mice.
abstract: The role of mast cells in polymorphonuclear leukocyte (PMN) influx in Ag-antibody complex-induced peritonitis was evaluated in mast cell-deficient WBB6F1-W/Wv (W/Wv) mice and their normal littermates, WBB6F1-+/+ (+/+). Peritoneal cell influx was evaluated after i.p. injection of preformed immune complexes. The first significant elevation in the PMN count over PBS-treated controls in +/+ mice was observed 2 h after stimulation. During the period of maximum leukocyte concentrations (6 to 10 h), the increase in total cell count was 5-fold and in PMN 25-fold. In W/Wv mice the PMN influx started 2 h later than in the +/+ mice, and the maximum response (8 to 10 h) was only 50% of that in controls. Reconstitution of mast cells in W/Wv mice for 2 wk or more restored the PMN response to immune complexes. Mast cell release due to AG-antibody complexes was evaluated by measuring fluorescence intensity after berberine sulfate staining for heparin in mast cells from unstimulated as well as stimulated +/+ mice. There was a significant decrease in fluorescence intensity as early as 15 min after stimulation. By 30 min the fluorescence intensity had declined by 65%. This indicates extensive mast cell release that started before PMN mobilization. These experiments demonstrate that mast cells make a significant contribution to immune complex-induced inflammation.
authors.affiliation: Department of Pharmacology, Washington University School of Medicine, St. Louis, MO 63110.
authors.forename: B F
authors.initials: BF
authors.lastname: Ramos
authors.forename: R
authors.initials: R
authors.lastname: Qureshi
authors.forename: K M
authors.initials: KM
authors.lastname: Olsen
authors.forename: B A
authors.initials: BA
authors.lastname: Jakschik
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex; D015550:Cell Degranulation; D002465:Cell Movement; D004306:Dose-Response Relationship, Immunologic; D006493:Heparin / Q000032:analysis; D007116:Immunization, Passive; D007249:Inflammation / Q000276:immunology; D008407:Mast Cells / Q000502:physiology*; D051379:Mice; D008856:Microscopy, Fluorescence; D009504:Neutrophils / Q000276:immunology*; D010538:Peritonitis / Q000276:immunology*
chemical_list: D000936:Antigen-Antibody Complex; D006493:Heparin