The monoclonal antibody AC1.59 defines a new polymorphic determinant on HLA-DR molecules.
2-dimensional gel electrophoresis analysis and sequential immunoprecipitation studies have shown that the monoclonal antibody (MoAb) AC1.59 recognizes a gene product of the HLA-DR locus. The determinant defined by the MoAb AC-1.59 has an unusual distribution on HLA-DR allospecificities, since it is expressed by HLA-DR1, DRw8, DRw9 antigens and by subtypes of HLA-DRw6 and HLA-DR4 antigens. In the latter allospecificity the expression of the MoAb AC1.59 defined determinant correlates with the cellularly defined HLA-Dw4 alloantigen. The present investigation provides evidence of serological heterogeneity within HLA-DR alloantigens. Furthermore these results suggest that monoclonal antibodies can sharpen the discriminatory power of serological assays to dissect the heterogeneity and complexity of HLA-DR alloantigens.
T
T
Crepaldi
P
P
Richiardi
F
F
Malavasi
A O
AO
Carbonara
M
M
Igarashi
S
S
Ferrone
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000276:immunology / Q000302:isolation & purification; D006684:HLA-DR Antigens; D015804:HLA-DR4 Antigen; D015808:HLA-DR6 Antigen; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006684:HLA-DR Antigens; D015804:HLA-DR4 Antigen; D015808:HLA-DR6 Antigen; D000949:Histocompatibility Antigens Class II