Epidermolytic toxin from Staphylococcus aureus binds to filaggrins.
The affinity of epidermolytic toxin from Staphylococcus aureus for proteins from the target tissue has been tested by a Western blotting procedure. Particular proteins in a 1 M phosphate extract of epidermis reacted on nitrocellulose blots with a probe prepared by the conjugation of toxin with peroxidase. Protein extracted into 50 mM Tris-HCl did not react. The probe detected profilaggrin, filaggrin and a smaller unidentified polypeptide. It is suggested that the interaction is relevant to the mode of action of the toxin.
Smith
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Bailey
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D000818:Animals; D001427:Bacterial Toxins / Q000378:metabolism*; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D005083:Exfoliatins / Q000378:metabolism*; D000091344:Filaggrin Proteins; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D007633:Keratins / Q000378:metabolism*; D051379:Mice; D010209:Paper; D010750:Phosphoproteins / Q000378:metabolism*; D011485:Protein Binding; D011498:Protein Precursors / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000378:metabolism*
D001427:Bacterial Toxins; D005083:Exfoliatins; D000091344:Filaggrin Proteins; D007381:Intermediate Filament Proteins; D010750:Phosphoproteins; D011498:Protein Precursors; C001500:keratohyalin; D007633:Keratins; D003101:Collodion