Effect of gramicidin A and thallium ions on cation effluxes in frog sartorius muscle.
The effluxes of potassium, rubidium, sodium and lithium from the sartorius muscle of Rana temporaria in magnesium-Ringer solution free of sodium and potassium have been studied with the flame-emission technique. The channel-forming antibiotic gramicidin A (2.5 X X10(-7)-1 X 10(-6) mol/l) enhanced the efflux of potassium and rubidium and increased the rate constants of these effluxes. Gramicidin had small if any effect on sodium and lithium effluxes and rate constants. After 60-100 min in a gramicidin-containing medium, the potassium efflux and the corresponding rate constant reached a steady-state level. This steady-state value depended on gramicidin concentration. Effect of gramicidin on both the potassium efflux and the rate constant was partially reversible. Thallium ions (2.5 X 10(-3) and 5 X 10(-3) mol/l) in sodium- and potassium- free magnesium Ringer solution caused a large increase in effluxes of all the cations examined (K+, Rb+ and Na+) both in presence and absence of gramicidin. Possible mechanisms of gramicidin and thallium effects are discussed.
N E
NE
Shvinka
F V
FV
Toropova
D000818:Animals; D002414:Cations, Monovalent; D002463:Cell Membrane Permeability / Q000187:drug effects; D006096:Gramicidin / Q000494:pharmacology*; D009132:Muscles / Q000187:drug effects*; D011896:Rana temporaria; D013793:Thallium / Q000494:pharmacology*
D002414:Cations, Monovalent; D006096:Gramicidin; D013793:Thallium