Reversible changes in rat spermatogenesis induced by an antifertility substance (Gossypol). A histochemical report.
Male rats received Gossypol 30 mg/kg body mass daily through intubation for 30 and 60 d. The testes were processed for histological and histochemical lipid staining, thiamine pyrophosphatase (TPPase), and alpha-glycerophosphate dehydrogenase (alpha-GPDH)--examinations. Gossypol induced effects were: various types of deformations of elongated spermatids, and their loss of orientation towards the lamina propria during the maturation phase; displacement of germ cells and occasionally also of Sertoli cells towards the lumen of the seminiferous tubule in form of a "puff". TPPase was observed in the supranuclear region of dislocated round spermatids and pachytene spermatocytes. alpha-GPDH was observed in the split pieces of mid- and tail pieces of elongated spermatids enclosed in the "puff". The induced effects were reversible.
Haider
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Passia
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Chen
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D000818:Animals; D003594:Cytoplasmic Granules / Q000032:analysis; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D006072:Gossypol / Q000494:pharmacology*; D008055:Lipids / Q000032:analysis; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012671:Seminiferous Tubules / Q000032:analysis; D012708:Sertoli Cells / Q000032:analysis; D013091:Spermatogenesis / Q000187:drug effects*; D013194:Staining and Labeling / Q000379:methods; D013737:Testis / Q000032:analysis* / Q000187:drug effects / Q000473:pathology; D013834:Thiamine Pyrophosphatase / Q000378:metabolism
D008055:Lipids; D005993:Glycerolphosphate Dehydrogenase; D013834:Thiamine Pyrophosphatase; D006072:Gossypol