[The detection of the surface antigens of Trichomonas vaginalis by using the scanning electron microscope].
The method for the detection of surface antigens, based on antibody aggregation phenomenon, was used in scanning microscopy of T. vaginalis. After Trichomonas treatment with immune blood serum diluted 1:5000--1:10,000 aggregates were detected, consisting of subunits 30-80 nm in diameter, localized on the protozoal cilia, projections, and, less frequently, body. Analysis of the results following the material treatment with colloid gold-conjugated anti-IgG permits a conclusion that the detected aggregates are antibodies to immunogenic surface antigens of T. vaginalis.
Bednova
V N
VN
Bragina
E E
EE
Kaptseva
G S
GS
D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis; D000953:Antigens, Protozoan / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis; D006801:Humans; D007120:Immunochemistry; D007074:Immunoglobulin G / Q000032:analysis; D007150:Immunohistochemistry; D008855:Microscopy, Electron, Scanning; D014245:Trichomonas Infections / Q000175:diagnosis; D014246:Trichomonas vaginalis / Q000276:immunology* / Q000648:ultrastructure
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D007074:Immunoglobulin G
Vyiavlenie antigenov poverkhnosti vlagalishchnykh trikhomonad s pomoshch'iu skaniruiushchego élektronnogo mikroskopa.