Binding and immunological properties of a synthetic peptide corresponding to the phosphorylcholine-binding region of C-reactive protein.
A synthetic peptide corresponding to amino acid residues 47-63 of human C-reactive protein (CRP) was synthesized and evaluated for its ability to bind phosphorylcholine (PC) and to react with mAb specific for the PC-binding region of CRP. The PC-binding peptide displayed Ca2(+)-independent binding specific for PC and was able to compete against CRP for PC in the presence of Ca2+ ions. The synthetic peptide, like CRP, binds to the extracellular matrix protein fibronectin and the basement membrane protein laminin. The PC-binding peptide was recognized by those mAb generated against the intact CRP molecule that bind at, or near, the functional PC-binding region. In addition, several mAb to the T-15 idiotype present on mouse antibodies specific for PC, recognize an epitope(s) on the PC-binding peptide. Therefore, the 17 amino acid synthetic peptide shares both functional binding activity and antigenicity with the corresponding functional region within the CRP molecule.
Swanson
S J
SJ
Department of Microbiology, Ohio State University, Columbus.
Mortensen
R F
RF
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D001665:Binding Sites; D001667:Binding, Competitive; D002097:C-Reactive Protein / Q000302:isolation & purification / Q000378:metabolism*; D002794:Choline / Q000031:analogs & derivatives*; D006801:Humans; D007130:Immunoglobulin Idiotypes; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology / Q000378:metabolism*; D010767:Phosphorylcholine / Q000378:metabolism*; D012995:Solubility
D000911:Antibodies, Monoclonal; D007130:Immunoglobulin Idiotypes; D010455:Peptides; D010767:Phosphorylcholine; D002097:C-Reactive Protein; D002794:Choline