Interleukin 1 increases the production of endothelin-1 by cultured endothelial cells.
We examined the effect of human recombinant interleukin 1 (IL-1) on the production of endothelin-1 by cultured porcine endothelial cells. The induction of endothelin-1 mRNA began within 1 hr of exposure to IL-1, showed twin peaks at 4 and 24 hr, and declined thereafter. Enzyme-linked immunosorbent assay revealed that the amount of endothelin-1 peptide in conditioned media was also increased by IL-1 in a dose- and time-dependent manner. Our results suggested that IL-1, a macrophage-derived cytokine, may affect the contraction and proliferation of vascular smooth muscle cells by stimulating the production of endothelin by endothelial cells.
Third Department of Internal Medicine, University of Tokyo, Japan.
M
M
Yoshizumi
H
H
Kurihara
T
T
Morita
T
T
Yamashita
Y
Y
Oh-hashi
T
T
Sugiyama
F
F
Takaku
M
M
Yanagisawa
T
T
Masaki
Y
Y
Yazaki
D000818:Animals; D001013:Aorta, Thoracic; D015152:Blotting, Northern; D002478:Cells, Cultured; D016232:Endothelins; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology*; D007700:Kinetics; D010452:Peptide Biosynthesis; D010455:Peptides / Q000235:genetics; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors; D011726:Pyridinium Compounds / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000494:pharmacology; D013552:Swine; D014158:Transcription, Genetic / Q000187:drug effects
D016232:Endothelins; D007375:Interleukin-1; D010455:Peptides; D010972:Platelet Activating Factor; D011726:Pyridinium Compounds; D012333:RNA, Messenger; D011994:Recombinant Proteins; C053321:CV 6209; D007213:Indomethacin