Incorporation of [3H]glucosamine into keratin-related polypeptides in pig epidermis.
Metabolic labelling studies have provided evidence for glycosylated keratins in cultured pig epidermis. [3H]Glucosamine was incorporated into five major particulate polypeptides of Mr 68 000, 61 000, 57 000, 53,000 and 48,000. Radioactivity was present in protein-bound carbohydrate. Non-enzymic glycation was excluded. Labelling was largely unaffected by tunicamycin indicating that radioactivity was incorporated mainly into O-linked oligosaccharides. These [3H]glucosamine-labelled components were closely related to keratins since they had a similar electrophoretic mobility to polypeptides of purified pig prekeratin, they were immunoprecipitated by anti-prekeratin serum and they were incorporated into reconstituted, intermediate-sized, keratin filaments.
I A
IA
King
D000818:Animals; D002850:Chromatography, Gel; D004817:Epidermis / Q000378:metabolism*; D005944:Glucosamine / Q000378:metabolism*; D007163:Immunosorbent Techniques; D007633:Keratins / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism; D008970:Molecular Weight; D010455:Peptides / Q000378:metabolism*; D011498:Protein Precursors / Q000378:metabolism; D013552:Swine
D010455:Peptides; D011498:Protein Precursors; C004818:prekeratin; D007633:Keratins; D007930:Leucine; D005944:Glucosamine