ras proteins can induce meiosis in Xenopus oocytes.
Injection of human H-ras protein induces maturation of Xenopus oocytes; that is, progression from prophase to metaphase of meiosis. The oncogenic protein encoded by H-rasval12 is nearly a 100-fold more potent than the protein encoded by the wild-type gene. We do not observe any measurable increase or decrease in cyclic AMP concentration in injected oocytes, and the effects of H-ras protein are only partially blocked by cholera toxin. Our results suggest that not all, if any, of the effects of H-rasval12 protein in this system are mediated by adenylate cyclase.
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Birchmeier
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Broek
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Wigler
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D002772:Cholera Toxin / Q000494:pharmacology; D000242:Cyclic AMP / Q000502:physiology; D005260:Female; D006160:Guanosine Triphosphate / Q000502:physiology; D006801:Humans; D008540:Meiosis; D009363:Neoplasm Proteins / Q000235:genetics / Q000502:physiology*; D009865:Oocytes / Q000201:enzymology / Q000502:physiology*; D009866:Oogenesis / Q000187:drug effects; D011374:Progesterone / Q000494:pharmacology; D016283:Proto-Oncogene Proteins p21(ras); D014982:Xenopus laevis
D009363:Neoplasm Proteins; D011374:Progesterone; D006160:Guanosine Triphosphate; D002772:Cholera Toxin; D000242:Cyclic AMP; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras); D000262:Adenylyl Cyclases; D015056:1-Methyl-3-isobutylxanthine