Sensory and autonomic innervation of non-hairy and hairy human skin. An immunohistochemical study.
Non-hairy and hairy human skin were investigated with the use of the indirect immunohistochemical technique employing antisera to different neuronal and non-neuronal structural proteins and neurotransmitter candidates. Fibers immunoreactive to antisera against neurofilaments, neuron-specific enolase, myelin basic protein, protein S-100, substance P, neurokinin A, neuropeptide Y, tyrosine hydroxylase and vasoactive intestinal polypeptide hydroxylase and vasoactive intestinal polypeptide (VIP) were detected in the skin with specific distributional patterns. Neurofilament-, neuron-specific enolase-, myelin basic protein-, protein S-100, neuropeptide Y-, tyrosine hydroxylase- and vasoactive intestinal polypeptide (VIP)-like immunoreactivities were found in or in association with sensory nerves; moreover, neuron-specific enolase-, myelin basic protein-, protein S-100, neuropeptide Y-, tyrosine hydroxylase- and vasoactive intestinal polypeptide (VIP)-like immunoreactivities occurred in or in association with autonomic nerves. It was concluded that antiserum against neurofilaments labels sensory nerve fibers exclusively, whereas neuron-specific enolase-, myelin basic protein- and protein S-100-like immunoreactivities are found in or in association with both sensory and autonomic nerves. Substance P- and neurokinin A-like immunoreactivities were observed only in sensory nerve fibers, and neuropeptide Y- and tyrosine hydroxylase-like immunoreactivities occurred only in autonomic nerve fibers, whereas vasoactive intestinal polypeptide (VIP)-like immunoreactivities was seen predominantly in autonomic nerves, but also in some sensory nerve fibers.
Björklund
H
H
Dalsgaard
C J
CJ
Jonsson
C E
CE
Hermansson
A
A
D001338:Autonomic Fibers, Postganglionic / Q000032:analysis*; D006197:Hair / Q000294:innervation*; D006801:Humans; D007382:Intermediate Filaments / Q000032:analysis; D009411:Nerve Endings / Q000032:analysis; D009419:Nerve Tissue Proteins / Q000032:analysis; D015288:Neurokinin A; D009475:Neurons, Afferent / Q000032:analysis*; D009478:Neuropeptide Y; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D012867:Skin / Q000294:innervation*; D013373:Substance P / Q000032:analysis; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
D009419:Nerve Tissue Proteins; D009478:Neuropeptide Y; D013373:Substance P; D015288:Neurokinin A; D014446:Tyrosine 3-Monooxygenase; D010751:Phosphopyruvate Hydratase