Localization of a phytochrome-responsive element within the upstream region of pea rbcS-3A.
The pea rbcS-3A promoter with a 5' deletion to -166 (-166 rbcS-3A) contains two GT-1-binding sites. Mutational analyses demonstrated that a decrease in affinity for GT-1 correlates with reduced promoter activity. Transcription of -166 rbcS-3A in transgenic etiolated seedlings is induced by red light and suppressed by far-red light, indicating that it contains a phytochrome-responsive element.
Laboratory of Plant Molecular Biology, Rockefeller University, New York, New York 10021-6399.
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Gilmartin
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Chua
D001483:Base Sequence; D001665:Binding Sites; D004274:DNA, Recombinant; D007887:Fabaceae / Q000235:genetics*; D017343:Genes, Plant; D008027:Light; D008969:Molecular Sequence Data; D010834:Phytochrome / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D010946:Plants, Medicinal; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D010940:Plant Proteins; D010834:Phytochrome