Molecular forms of cholecystokinin in rat intestine.
The predominant immunoreactive cholecystokinin (CCK) forms in acid extracts of rat intestine eluted from reverse-phase high-performance liquid chromatography columns in the positions of CCK-8, CCK-33/39, and CCK-58. Control experiments indicated that smaller CCK forms did not arise from artifactual degradation of large CCK forms. Less than 10% of CCK-8 added to and extracted from intestine was recovered in acid; CCK-8 could not be recovered by subsequent alkaline extraction, but subsequent urea extraction yielded approximately 25% of the added peptide. This suggests that CCK binds to proteins during acid extraction and that the preponderance of large CCK forms in acid extracts is not due to inhibition of CCK degradation but results from poor extraction of small CCK forms. No evidence for a CCK-22-like peptide was found in acid or subsequent urea extracts of rat intestine, suggesting CCK posttranslational processing in adult rats is like that in humans and dogs.
Research Service, Veterans Administration Medical Center, Kansas City, Missouri 64128.
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Turkelson
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Solomon
D000818:Animals; D002766:Cholecystokinin / Q000031:analogs & derivatives* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D005755:Gastrins / Q000302:isolation & purification; D007106:Immune Sera; D007422:Intestines / Q000032:analysis*; D008297:Male; D009130:Muscle, Smooth / Q000032:analysis; D011499:Protein Processing, Post-Translational; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D005755:Gastrins; D007106:Immune Sera; D002766:Cholecystokinin