Nucleic acid staining with the methyl green-pyronin method. A comparison of the use of pure dyes and commercially available dyes.
We compared the staining obtained using commercially available pyronin Y samples with that obtained using pure pyronin Y in a standardized methyl green-pyronin procedure. In addition, the importance of the dye content of the anhydrous dye was investigated by varying the dye content by the addition of pure pyronin Y to one of the commercially available pyronin Y samples. We found that, for routine histological work, commercially available pyronin Y samples may produce acceptable results provided the sample can be shown by spectrophotometry to contain at least 43% pyronin Y.
Andersen
A P
AP
Lyon
H
H
Jakobsen
P
P
Høyer
P E
PE
D000818:Animals; D005557:Formaldehyde; D008739:Methyl Green; D009696:Nucleic Acids / Q000032:analysis*; D011754:Pyronine; D051381:Rats; D012394:Rosaniline Dyes; D013194:Staining and Labeling; D014966:Xanthenes
D009696:Nucleic Acids; D012394:Rosaniline Dyes; D014966:Xanthenes; D005557:Formaldehyde; D008739:Methyl Green; D011754:Pyronine