A simple and improved method for direct and indirect immunofluorescent staining.
Several technics for immunofluorescent staining have been described in the literature and are presently used by laboratories performing direct and indirect tissue immunofluorescence. The purpose of this communication is to describe a simple immunofluorescent technic that uses a five-slide polypropylene slide mailer containing the diluted conjugated antisera. This technic eliminates the need for an incubation chamber, decreases background staining, and uses less antiserum.
Grimwood
R E
RE
Huff
J C
JC
Weston
W
W
D005455:Fluorescent Antibody Technique; D006801:Humans; D008722:Methods; D012867:Skin / Q000276:immunology; D013194:Staining and Labeling