The formylmethanofuran:tetrahydromethanopterin formyltransferase from Methanobacterium thermoautotrophicum delta H. Nucleotide sequence and functional expression of the cloned gene.
The formylmethanofuran:tetrahydromethanopterin formyltransferase (FTR) from Methanobacterium thermoautotrophicum delta H was cloned and its sequence was determined. The clone was contained on a 4.8-kilobase BamHI fragment of M. thermoautotrophicum DNA ligated into pBR329. When this fragment was subcloned into the phagemid pTZ18R, a functional enzyme was synthesized under control of the lac promoter. Sequence analysis revealed the presence of a ribosome binding site and a possible terminator structure. The absence of an identifiable promoter lends credibility to the open reading frame which is present 5' to ftr. The ftr gene encodes an acidic protein with a calculated molecular weight of 31,401. The sequence of FTR does not appear to be homologous to any other sequenced proteins, including proteins which use pterin substrates.
DiMarco
A A
AA
Department of Microbiology, University of Illinois, Urbana 61801.
Sment
K A
KA
Konisky
J
J
Wolfe
R S
RS
D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D015245:Deoxyribonuclease BamHI; D004926:Escherichia coli / Q000235:genetics; D019605:Euryarchaeota / Q000201:enzymology* / Q000235:genetics; D058973:Folate Receptors, GPI-Anchored; D015870:Gene Expression; D019877:Hydroxymethyl and Formyl Transferases; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D010957:Plasmids; D011956:Receptors, Cell Surface; D015183:Restriction Mapping; D014166:Transferases / Q000235:genetics*
D002352:Carrier Proteins; D004269:DNA, Bacterial; D058973:Folate Receptors, GPI-Anchored; D015345:Oligonucleotide Probes; D011956:Receptors, Cell Surface; D014166:Transferases; D019877:Hydroxymethyl and Formyl Transferases; C050840:formylmethanofuran-tetrahydromethanopterin formyltransferase; D015245:Deoxyribonuclease BamHI