The composition of liver chromatin proteins from embryos, chicks and adult chickens.
The chemical composition of chromatin from the livers of 12-, 15- and 19-day-old embryos, of 1-day-old chicks and of adult chickens was analysed. The process of embryonic development is accompanied by an increase in non-histone chromatin proteins and chromatin RNA, as well as in the phosphorus content of chromatin phosphoproteins. The amount of these components decreases in the livers of 1-day-old chicks and adults. Two-dimensional polyacrylamide gel electrophoresis of acid-soluble chromatin proteins showed an increase in the amount of the H1 histone in 19-day-old embryos and adult chickens. Non-histone proteins of embryo liver chromatin showed a high content of the fraction of Mr of about 40 000; this was not the case for adult chickens. The non-histone protein fraction of Mr of about 120 000, characteristic of adult chicken liver proteins, was not found in the livers of 12- and 15-day-old embryos. Non-histone chromatin proteins isolated from the livers of animals of different age exhibited also quantitative differences.
Luboń
H
H
Pałyga
J
J
Górnicka-Michalska
E
E
D000367:Age Factors; D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D002843:Chromatin / Q000032:analysis*; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis*; D004247:DNA / Q000032:analysis; D006657:Histones / Q000032:analysis; D008099:Liver / Q000032:analysis* / Q000648:ultrastructure; D010750:Phosphoproteins / Q000032:analysis; D012313:RNA / Q000032:analysis
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D010750:Phosphoproteins; D012313:RNA; D004247:DNA