Differential expression of fucosyl GM1 and a disialoganglioside with a NeuAc alpha 2-6GalNAc linkage (GD1e) in various rat ascites hepatoma cells.
A distinct difference in ganglioside composition among various rat ascites hepatomas and Yoshida sarcoma was observed on TLC-immunostaining with anti-fucosyl GM1 antibody, and chemical and enzymatic analyses. Yoshida sarcoma and ascites hepatomas, AH13, AH66F and AH66, but not the other 9 tumor cell lines investigated, specifically contained a disialoganglioside, NeuAc alpha 2-3Gal beta 1-3(NeuAc alpha 2-6)GalNAc beta 1-4Gal beta 1-4Glc beta 1-1ceramide (GD1e), whereas the 9 ascites hepatoma cells without GD1e contained fucosyl GM1. The differential expression of fucosyl GM1 and GD1e in various tumor cell lines indicates that different cell lineages express distinct metabolic pathways for gangliosides, and that the gangliosides are useful markers for distinguishing tumor cell lines.
Iwamori
M
M
Sunada
S
S
Ishihara
E
E
Moki
M
M
Fujimoto
S
S
Nagai
Y
Y
D000818:Animals; D002460:Cell Line; D002855:Chromatography, Thin Layer; D005677:G(M1) Ganglioside / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology; D005732:Gangliosides / Q000032:analysis* / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000032:analysis*; D051381:Rats; D012517:Sarcoma, Yoshida / Q000032:analysis; D013194:Staining and Labeling
D005732:Gangliosides; D005677:G(M1) Ganglioside; C039123:fucosyl GM1 ganglioside