Direct localization and quantitation of aminoacyl-tRNA synthetase activity in polyacrylamide gel.
A simple procedure for the direct localization and quantitation of aminoacyl-tRNA synthetase activity in polyacrylamide gel is described. The method is based on the white precipitation of Ca-PPi. The white bands of the precipitated calcium salt are clearly visible when viewed against a dark background and can be photographed or scanned.
Chang
G G
GG
Denq
R Y
RY
Pan
F
F
D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification*; D000818:Animals; D002131:Calcium Pyrophosphate; D011232:Chemical Precipitation; D004589:Electrophoresis, Disc; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000201:enzymology; D011817:Rabbits; D013194:Staining and Labeling; D014637:Valine-tRNA Ligase / Q000302:isolation & purification
D000604:Amino Acyl-tRNA Synthetases; D014637:Valine-tRNA Ligase; D002131:Calcium Pyrophosphate