A specific immunological probe for the major myelin proteolipid. Confirmation of a deletion in DM-20.
Major myelin proteolipid (MMPL, also called PLP) and DM-20 are the two major intrinsic membrane proteins of CNS myelin. A specific immunological probe was obtained for MMPL by raising antibodies against the synthetic tridecapeptide 117-129 of MMPL. Antibodies against this peptide reacted with the MMPL but did not cross react with DM-20, while both proteolipids had been shown previously to be recognized by antibodies directed against the C-terminal hexapeptide of MMPL. This is in accordance with previous findings showing that DM-20 differs only from MMPL by a deletion of residues 100-140 (+/- few units). Furthermore, this site-specific immunological probe also recognizes MMPL in its native form in oligodendrocytes in primary glial cell cultures.
E
E
Trifilieff
B
B
Luu
J L
JL
Nussbaum
G
G
Roussel
A
A
Espinosa de los Monteros
J M
JM
Sabatier
J
J
Van Rietschoten
D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D009185:Myelin Proteins / Q000032:analysis* / Q000276:immunology; D018991:Myelin Proteolipid Protein; D009419:Nerve Tissue Proteins; D009457:Neuroglia / Q000378:metabolism; D011510:Proteolipids / Q000032:analysis* / Q000276:immunology; D011817:Rabbits; D051381:Rats
D009185:Myelin Proteins; D018991:Myelin Proteolipid Protein; D009419:Nerve Tissue Proteins; C489071:Plp1 protein, rat; D011510:Proteolipids