Mechanism controlling the genetic restrictions of an NP-specific suppressor factor that inhibits B cell responses.
The mechanism of B cell suppression by a T cell hybridoma-derived monoclonal effector suppressor factor (TsF3) was studied in the 4-hydroxy-3-nitrophenyl acetyl (NP) system. The NP-specific effector suppressor cells that produce TsF3 are Lyt-1-, 2+, I-J+, NP-binding T cells and are induced by immunization with NP conjugates. Monoclonal TsF3 inhibits both T cell activity as measured by suppression of contact sensitivity responses and B cell function as measured by suppression of antibody production to both T-independent and T-dependent antigens. The present studies were designed to specifically investigate the mechanisms and genetic restrictions that govern the interactions between TsF3 and its target cells in the plaque-forming cell (PFC) response. The results show that the target of TsF3 is a splenic adherent cell. Suppression will occur only if the restriction specificity of the TsF3 matches the H-2 genotype of the adherent population. Once this TsF3-adherent cell interaction has occurred, suppression of NP-specific B cells can occur across an H-2 barrier. The data also demonstrate that Igh-linked gene products do not appear to play a part in the TsF3-mediated suppression of in vitro PFC responses, which contrasts with the requirements for regulation of T cell-mediated contact sensitivity responses.
Hausman
P B
PB
Sherr
D H
DH
Dorf
M E
ME
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D000939:Epitopes / Q000235:genetics; D006183:H-2 Antigens / Q000235:genetics*; D006462:Hemolytic Plaque Technique; D006825:Hybridomas / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007700:Kinetics; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000145:classification / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009596:Nitrophenols / Q000276:immunology*; D010648:Phenylacetates; D013154:Spleen / Q000166:cytology; D013491:Suppressor Factors, Immunologic; D050378:T-Lymphocytes, Regulatory / Q000145:classification / Q000276:immunology
D000939:Epitopes; D006183:H-2 Antigens; D007143:Immunoglobulin Heavy Chains; D008222:Lymphokines; D009596:Nitrophenols; D010648:Phenylacetates; D013491:Suppressor Factors, Immunologic; C026242:4-hydroxy-5-nitrophenyl acetic acid