In vitro release of pregnancy-associated plasma protein-A (PAPP-A) by human endometrial cells.
Evidence has now accumulated to sustain the idea that pregnancy-associated plasma protein-A (PAPP-A) is not pregnancy specific: it can be localized immunohistochemically in nonpregnant human endometrium. Thus, it was of interest to see if a primary cell culture of human endometrial cells was capable of producing PAPP-A. Immunoreactive PAPP-A-like material was detected in the culture medium of glandular as well as stromal cells. The presence of PAPP-A in endometrial culture medium was specific and could not be attributed to a nonspecific protease effect on the tracer used in radioimmunoassay. The production rate of PAPP-A by stromal cells could be enhanced by the addition of medroxyprogesterone acetate (MPA). Estradiol (E2) alone or in combination with MPA did not modify the production rate of PAPP-A. The production rate of PAPP-A by glandular cells remained unchanged after treatment with MPA and/or E2. These results confirm that in nonpregnant women PAPP-A is a progesterone-dependent protein produced by the endometrium.
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Bischof
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Tseng
D002478:Cells, Cultured; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D006801:Humans; D008525:Medroxyprogesterone / Q000031:analogs & derivatives / Q000494:pharmacology; D017258:Medroxyprogesterone Acetate; D011257:Pregnancy Proteins / Q000378:metabolism*; D011266:Pregnancy-Associated Plasma Protein-A / Q000276:immunology / Q000378:metabolism*; D011863:Radioimmunoassay; D012636:Secretory Rate / Q000187:drug effects
D011257:Pregnancy Proteins; D004958:Estradiol; D017258:Medroxyprogesterone Acetate; D011266:Pregnancy-Associated Plasma Protein-A; D008525:Medroxyprogesterone