Monoclonal antibodies to rat C apolipoproteins: production and characterization of a unique antibody whose binding to apoC-I is inhibited by nonionic detergents.
Four monoclonal antibodies to rat apo (apolipoproteins) C were produced. Three of the monoclonals reacted to apoC-I and one to apoC-III. The IgG monoclonals LRB 21 and LRB 45 recognized a spatially close together or identical apoC-I epitope. The monoclonal LRB 80, however, recognized an epitope that is close to, but distinct from, that recognized by LRB 45 and LRB 21. The antibody LRB 45 recognized an apoC-I epitope that is specific for rat apoC-I, and the antibody did not cross-react with dog or human lipoproteins. Rat apoC-I could be detected in all lipoprotein density fractions, but not in the d greater than 1.21 g/ml fraction. Freezing and thawing of serum did not alter the antibody antigen binding. However, lipolysis of whole serum resulted in a 30% increase in antigenic epitope expression. Antibody antigen reaction could be inhibited by subcritical micellar concentration of nonionic detergents. The inhibition was specific but could be partially reversed if lipid-containing serum was used as a dilution buffer. On feeding animals a diet of olive oil and cholesterol for 2 weeks, apoC-I levels decreased.
Wong
L
L
Anderson
P D
PD
Gallaher
W R
WR
Roheim
P S
PS
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000937:Antigen-Antibody Reactions / Q000187:drug effects; D053302:Apolipoprotein C-I; D001056:Apolipoproteins C / Q000097:blood / Q000276:immunology*; D001666:Binding Sites, Antibody / Q000187:drug effects; D055598:Chemical Phenomena; D002621:Chemistry; D003101:Collodion; D003429:Cross Reactions; D003902:Detergents / Q000494:pharmacology*; D004032:Diet; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D006825:Hybridomas; D008049:Lipase / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D013501:Surface-Active Agents / Q000494:pharmacology*
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D053302:Apolipoprotein C-I; D001056:Apolipoproteins C; D003902:Detergents; D000939:Epitopes; D013501:Surface-Active Agents; D003101:Collodion; D008049:Lipase