[Technical note on the isolation of HIV from peripheral lymphomonocytes: use of PHA].
As a part of ongoing study about the role that different technical factors may play in influencing the sensibility of HIV isolation procedures, the authors have evaluated the effects of PHA stimulation of infected cells on HIV replication in cell cultures. Data presented demonstrate that the use of PHA in cell cultures for HIV isolation causes a slower viral replication and, in some cases, inhibits HIV growth.
Fiore
J R
JR
Clinica di Malattie Infettive, Università di Bari.
Angarano
G
G
Fico
C
C
Fracasso
C
C
Carbonara
S
S
Pastore
G
G
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006679:HIV Seropositivity / Q000382:microbiology*; D015497:HIV-1 / Q000302:isolation & purification* / Q000502:physiology; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000382:microbiology*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D013997:Time Factors; D014773:Virology / Q000379:methods; D014779:Virus Replication / Q000187:drug effects*
D010835:Phytohemagglutinins
Note tecniche sull'isolamento dell'HIV da linfomonociti periferici: impiego della PHA.