Co-localization of calcitonin gene-related peptide-like immunoreactivity with substance P in cutaneous, vascular and visceral sensory neurons of guinea pigs.
Calcitonin gene-related peptide (CGRP) has been immunohistochemically co-localized with substance P (SP) in capsaicin-sensitive, varicose axons supplying the skin, viscera and cardiovascular system of the guinea pig. After treatment with colchicine in vitro, 82% of SP neurons in the dorsal root ganglia contained CGRP-like immunoreactivity while 96% of CGRP neurons were immunoreactive for SP. Both CGRP- and SP-like immunoreactive material are transported peripherally and centrally from dorsal root ganglia. Thus, in tissues such as the gut where there are intrinsic nerves containing SP but lacking CGRP, CGRP-like immunoreactivity is a useful means of specifically labelling axons of most sensory neurons containing SP.
I L
IL
Gibbins
J B
JB
Furness
M
M
Costa
I
I
MacIntyre
C J
CJ
Hillyard
S
S
Girgis
D000818:Animals; D015740:Calcitonin Gene-Related Peptide; D002319:Cardiovascular System / Q000294:innervation; D005123:Eye / Q000294:innervation; D005260:Female; D005455:Fluorescent Antibody Technique; D005727:Ganglia, Spinal / Q000378:metabolism; D006168:Guinea Pigs; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009475:Neurons, Afferent / Q000378:metabolism; D010525:Peripheral Nerves / Q000378:metabolism*; D012867:Skin / Q000294:innervation; D013373:Substance P / Q000378:metabolism*; D012668:Trigeminal Ganglion / Q000378:metabolism; D014566:Urogenital System / Q000294:innervation; D014599:Uterus / Q000294:innervation
D009419:Nerve Tissue Proteins; D013373:Substance P; D015740:Calcitonin Gene-Related Peptide