[Effect of the synthetic heparin antagonist, conidine oligomer 25, on the synthesis of nucleic acids in regenerating liver cells].
Localization of 14C-conidine oligomer (the synthetic antagonist of heparin) and of polyelectrolyte complex heparin-conidine oligomer 25 was studied in cells of regenerating liver tissue within 20 hrs after partial hepatectomy in rats. Effects of heparin, conidine oligomer 25 and of their polyelectrolyte complex on synthesis of nucleic acids were studied. Conidine oligomer 25 penetrated from circulation into hepatocytes and subcellular organelles of regenerating liver cells and was localized mainly in cytosol (75-88%). In nuclei and mitochondria of the hepatocytes 14C-conidine oligomer 25 was mostly found in the fraction of low density of the organelles. Polyanion heparin, polycation conidine oligomer 25 and their polymeric complex impaired replication and transcription processes.
Silaeva
S A
SA
Khatsernova
B Ia
BIa
Nikolaev
A Ia
AIa
Efimov
V S
VS
Rozkin
M Ia
MIa
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis*; D006493:Heparin / Q000494:pharmacology; D006494:Heparin Antagonists / Q000494:pharmacology*; D006498:Hepatectomy; D008099:Liver / Q000378:metabolism*; D008115:Liver Regeneration / Q000187:drug effects*; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism; D011108:Polymers / Q000494:pharmacology*; D012313:RNA / Q000096:biosynthesis*; D051381:Rats; D013154:Spleen / Q000378:metabolism
D006494:Heparin Antagonists; D011108:Polymers; D012313:RNA; C039164:conidine; D006493:Heparin; D004247:DNA
Vliianie sinteticheskogo antagonista geparina oligomera-25 konidina na sintez nukleinovykh kislot v kletkakh regeneriruiushcheĭ pecheni.