Molecular cloning of the mouse cell adhesion molecule uvomorulin: cDNA contains a B1-related sequence.
A clone (F20) containing coding sequences for the cell adhesion molecule uvomorulin was isolated by immunological techniques from cDNA library in the expression vector lambda gt11. The beta-galactosidase-uvomorulin fusion protein was used to affinity purify anti-uvomorulin antibodies. Affinity-purified antibodies recognized uvomorulin from cell lysates of embryonal carcinoma cells and reacted with the cell surface of embryonal carcinoma cells. The 1.8-kilobase cDNA insert hybridized to a single 4.3-kilobase poly(A)+ RNA species found only in cells expressing uvomorulin. Part of the nontranslated 3' sequences of the cloned uvomorulin cDNA is homologous to the interspersed B1 repeat of the mouse genome.
R
R
Schuh
D
D
Vestweber
I
I
Riede
M
M
Ringwald
U B
UB
Rosenberg
H
H
Jäckle
R
R
Kemler
D000818:Animals; D001483:Base Sequence; D015820:Cadherins; D002448:Cell Adhesion; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005455:Fluorescent Antibody Technique; D005796:Genes; D006023:Glycoproteins / Q000235:genetics* / Q000276:immunology; D007163:Immunosorbent Techniques; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D000509:alpha-Fetoproteins / Q000235:genetics
D015820:Cadherins; D006023:Glycoproteins; D012333:RNA, Messenger; D000509:alpha-Fetoproteins; D004247:DNA