Molecular cloning of bovine viral diarrhea viral sequences.
Bovine viral diarrhea virus (BVDV) genomic RNA was identified as a 12.5-kb single-stranded RNA molecule in both infected bovine embryonic kidney cells (BEK-1) and partially purified virions. BVD virion RNA was partially purified and used as a template for cDNA synthesis. BVDV-specific cDNA sequences were molecularly cloned and shown to hybridize to infected cell RNA but not to uninfected cell RNA or DNA. A single RNA species of 12.5 kb, representing the viral RNA genome, was detected in infected cells. A preliminary map of the BVDV specific cDNA clones was constructed and five major, nonoverlapping families were observed, accounting for approximately one-half of the viral genome.
A
A
Renard
C
C
Guiot
D
D
Schmetz
L
L
Dagenais
P P
PP
Pastoret
D
D
Dina
J A
JA
Martial
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002478:Cells, Cultured; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D001908:Diarrhea Viruses, Bovine Viral / Q000235:genetics*; D005814:Genes, Viral; D010576:Pestivirus / Q000235:genetics*; D011061:Poly A / Q000235:genetics; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D012367:RNA, Viral / Q000096:biosynthesis / Q000235:genetics; D014776:Virus Cultivation
D012333:RNA, Messenger; D012367:RNA, Viral; D011061:Poly A; D012313:RNA; D004247:DNA