Incorporation of labeled ribonucleic acid precursors into maternal and fetal rat tissues during pregnancy.
Tritium-labeled ribonucleic acid precursors, including cytidine, uridine, and orotic acid, were injected into rats with dated pregnancies (14 to 21 days) and virgin rats. The acid-insoluble counts indicating incorporation into fetal and placental tissues showed that the highest incorporation occurred with cytidine, particularly earlier in pregnancy. In contrast, uridine demonstrated a minor degree of incorporation but displayed facile and enhanced transplacental passage with duration of pregnancy as represented by acid-soluble counts. Orotic acid was minimally used by both fetal and placental tissues. The incorporation of labeled precursors into maternal liver, heart, and kidney demonstrated varying responses during the course of pregnancy.
Dorko
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ME
Hayashi
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D000818:Animals; D003562:Cytidine / Q000378:metabolism; D005260:Female; D005333:Fetus / Q000378:metabolism*; D007275:Injections, Intravenous; D008431:Maternal-Fetal Exchange; D009694:Nucleic Acid Precursors / Q000378:metabolism*; D009963:Orotic Acid / Q000378:metabolism; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D011270:Pregnancy, Animal; D012313:RNA / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D009694:Nucleic Acid Precursors; D014316:Tritium; D003562:Cytidine; D009963:Orotic Acid; D012313:RNA; D014529:Uridine