Cat-2 gene expression. Developmental control of translatable CAT-2 mRNA levels in maize scutellum.
Poly(A)+ RNA was isolated from maize scutella of different stages of post-germinative development and translated in vitro in a rabbit reticulocyte translation system. Immunoprecipitation of the translation products with CAT-2-specific antibody was used to quantitate the relative levels of translatable CAT-2 mRNA at each stage. The results show a close correlation between the developmental profile of Cat2 gene expression and the profile of CAT-2 mRNA levels. Evidence that the levels of CAT-2 mRNA are regulated by a temporal regulatory gene (Car1) is presented and the possible mechanism(s) of this regulation discussed.
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Kopczynski
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JG
Scandalios
D000818:Animals; D002374:Catalase / Q000235:genetics*; D005786:Gene Expression Regulation; D010944:Plants / Q000235:genetics*; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D003313:Zea mays / Q000235:genetics / Q000254:growth & development
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D002374:Catalase