Transport of a low molecular weight extracellular esterase into membrane vesicles of Candida lipolytica.
The low mol. wt extracellular esterase of Candida lipolytica is actively transported into membrane vesicles. In the absence of metabolic energy, a proton gradient can drive the transport process. The transport system does not accumulate the enzyme at peak levels due to the presence of a leak pathway.
G I
GI
Adoga
M
M
Mattey
D000255:Adenosine Triphosphate / Q000494:pharmacology; D001693:Biological Transport, Active / Q000187:drug effects; D002175:Candida / Q000201:enzymology*; D002462:Cell Membrane / Q000201:enzymology; D055598:Chemical Phenomena; D002621:Chemistry; D004492:Edetic Acid / Q000494:pharmacology; D004950:Esterases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007473:Ion Channels / Q000378:metabolism; D007700:Kinetics; D008623:Mercaptoethanol / Q000494:pharmacology; D008970:Molecular Weight; D011522:Protons; D011523:Protoplasts / Q000201:enzymology; D013329:Structure-Activity Relationship
D007473:Ion Channels; D011522:Protons; D008623:Mercaptoethanol; D000255:Adenosine Triphosphate; D004492:Edetic Acid; D004950:Esterases