Phosphorylation of carnitine palmitoyltransferase and activation by glucagon in isolated rat hepatocytes.
Effects of glucagon and forskolin on the phosphorylation and changes of activity of carnitine palmitoyltransferase (CPT) have been studied in isolated rat hepatocytes using anti-CPT immunoglobulin. When the activity was determined in lysed hepatocytes after glucagon or forskolin treatment, it was found to be stimulated 30-80% mainly through increased affinity for palmitoyl-CoA. By SDS electrophoresis of the immunoprecipitates, CPT subunit (Mr 69000) was noted to be phosphorylated 4-5-fold with glucagon (1.2 X 10(-7) M) and forskolin (0.1 mM) over control. These results indicate that hepatic ketogenesis is regulated with glucagon by phosphorylation of CPT through cAMP-dependent protein kinase.
Harano
Y
Y
Kashiwagi
A
A
Kojima
H
H
Suzuki
M
M
Hashimoto
T
T
Shigeta
Y
Y
D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography; D002334:Carnitine O-Palmitoyltransferase / Q000378:metabolism*; D005576:Colforsin; D004224:Diterpenes / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D005934:Glucagon / Q000494:pharmacology*; D007120:Immunochemistry; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D007657:Ketone Bodies / Q000096:biosynthesis; D008099:Liver / Q000201:enzymology*; D010455:Peptides / Q000032:analysis; D010766:Phosphorylation; D051381:Rats; D013194:Staining and Labeling
D004224:Diterpenes; D007328:Insulin; D007657:Ketone Bodies; D010455:Peptides; D005576:Colforsin; D005934:Glucagon; D000217:Acyltransferases; D002334:Carnitine O-Palmitoyltransferase