Effect of prostaglandins on interferon synthesis in murine macrophage-like cell lines.
Murine macrophage-like cell lines were used to determine whether exogenously added prostaglandins and endogenous prostaglandins suppress interferon (IFN) synthesis in macrophages. The amount of IFN produced by J774A.1 cells induced with bacterial lipopolysaccharide (LPS) was reduced by 0.1 and 1 microM PGE1 or PGE2. These prostaglandins also inhibited Newcastle disease virus (NDV) induced IFN production, but only at a concentration of 1 microM. Thromboxane B2 at 0.01 to 1 microM had no effect on IFN production. Cells treated before, during, or before and during IFN synthesis with 0.15 to 4.8 microM indomethacin to inhibit prostaglandin synthesis did not increase IFN yields. Indomethacin also had no effect on NDV-induced IFN production by P388D1 and PU5-1.8 cells, and these cells remained nonresponsive to LPS for IFN production. These results indicate that endogenous levels of cyclooxygenase-dependent metabolites of arachidonic acid do not regulate IFN synthesis in macrophages.
Salo
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RJ
Maddux
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NL
Bleam
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DK
D000527:Alprostadil / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D015232:Dinoprostone; D007213:Indomethacin / Q000494:pharmacology; D007372:Interferons / Q000096:biosynthesis* / Q000494:pharmacology; D007739:L Cells / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D009522:Newcastle disease virus; D011458:Prostaglandins E / Q000494:pharmacology*; D013929:Thromboxane B2 / Q000494:pharmacology*; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects
D008070:Lipopolysaccharides; D011458:Prostaglandins E; D013929:Thromboxane B2; D007372:Interferons; D000527:Alprostadil; D015232:Dinoprostone; D007213:Indomethacin