Nitroreductase reactions of the NADPH: adrenodoxin reductase and the adrenodoxin complex.
NADPH: adrenodoxin reductase (E.C. 1.18.1.2) and its complex with adrenodoxin catalyze the aerobic oxidation of NADPH by a number of substituted 2-nitrofurans, 5-nitroimidazoles and p-derivatives of nitrobenzene. The nitrocompounds are reduced via an initial single-electron transfer. Under anaerobic conditions nitrofurans are reduced to the corresponding amines. The rate constants of adrenodoxin oxidation by nitrocompounds vary from 4 x 10(5) to 3 x 10(2) M-1 s-1. A linear correlation between the rate constant logarithm and the single-electron reduction potential at pH 7.0 (E7(1)) of nitrocompounds was observed. The relation between the reactivity and the polarographic half-wave potential (E7(1/2)) is distorted. The reactivity of adrenodoxin reductase is two orders of magnitude lower than that of adrenodoxin.
Institute of Biochemistry, Lithuanian Academy of Sciences, Vilnius, Mokslininku, USSR.
J
J
Marcinkeviciene
N
N
Cenas
J
J
Kulys
S A
SA
Usanov
N M
NM
Sukhova
I S
IS
Selezneva
V F
VF
Gryazev
D000325:Adrenodoxin / Q000378:metabolism*; D005287:Ferredoxin-NADP Reductase / Q000378:metabolism*; D007700:Kinetics; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D009249:NADP / Q000378:metabolism*; D009578:Nitrobenzenes / Q000378:metabolism*; D009581:Nitrofurans / Q000378:metabolism*; D009593:Nitroimidazoles / Q000378:metabolism*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism
D009578:Nitrobenzenes; D009581:Nitrofurans; D009593:Nitroimidazoles; D000325:Adrenodoxin; D009249:NADP; D005287:Ferredoxin-NADP Reductase; D009247:NADH, NADPH Oxidoreductases; D010100:Oxygen