High-performance liquid chromatographic analysis of alpha-keto acids produced from amino acid metabolism in oral Bacteroides.
Profiles of metabolic alpha-keto acids were determined by a high-performance liquid chromatographic method and applied to characterization of oral black-pigmented Bacteroides. Each bacterial strain was incubated with amino acids in a chemically defined medium. After production alpha-keto acids were purified by hydrazide gel column treatment and converted to u.v.-absorbing derivatives. They were analysed by reversed-phase ion-pair chromatography. Black-pigmented Bacteroides species were differentiated into two groups according to production of aromatic alpha-keto acids. Bacteroides gingivalis, B. endodontalis and B. loescheii produced both p-hydroxyphenylpyruvic and phenylpyruvic acids. However, no such alpha-keto acids were produced by B. levii, B. intermedius and B. denticola. In addition, production profiles of several aliphatic alpha-keto acids (alpha-ketoglutaric, pyruvic, alpha-ketobutyric, alpha-ketoisovaleric, alpha-ketoisocaproic, and alpha-keto-beta-methylvaleric acids) separated each individual species in such groups. The present study offers useful chemotaxonomic information on amino acid metabolic activity of oral black-pigmented Bacteroides species.
Department of Dental Pharmacology, Asahi University School of Dentistry, Gifu, Japan.
H
H
Tsuchiya
M
M
Sato
K
K
Yamamoto
M
M
Yamauchi
H
H
Tani
I
I
Namikawa
N
N
Takagi
D000596:Amino Acids / Q000378:metabolism*; D001439:Bacteroides / Q000145:classification / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007651:Keto Acids / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D009055:Mouth / Q000382:microbiology; D013045:Species Specificity
D000596:Amino Acids; D007651:Keto Acids