Structural differences between plasma-membrane 5'-nucleotidase in different cell types as evidenced by antibodies.
Antibodies raised against bovine 5'-nucleotidase inhibit this enzyme as well as 5'-nucleotidase from other bovine tissues, showing common structure(s) between these proteins. However, an IgG fraction directed against the glucidic moiety of the liver enzyme did not cross-react with the enzyme from lymphocyte or caudate nuclei, a clear indication that within the same species the 5'-nucleotidase differs from one cell type to another. In addition, immunoblots after electrophoresis show that the previous antibodies recognize 5'-nucleotidase from human, mouse or chicken origin. However, only human 5'-nucleotidase activity can be inhibited by the antibodies. Thus at least three groups of antigenic determinants must exist on the 5'-nucleotidase: one related to the glucidic moiety of the glycoprotein whose binding inhibits the enzyme activity, another related to the catalytic site, as its binding also led to enzyme inhibition, and a last one of structural nature. It seems that the third group of determinant is common to many species, whereas the second one is more restricted.
J
J
Harb
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K
Meflah
S
S
Bernard
D015720:5'-Nucleotidase; D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex / Q000032:analysis; D002241:Carbohydrates / Q000494:pharmacology; D002417:Cattle; D002462:Cell Membrane / Q000201:enzymology; D002645:Chickens; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D051379:Mice; D009708:Nucleotidases / Q000276:immunology; D013045:Species Specificity; D014018:Tissue Distribution
D000906:Antibodies; D000936:Antigen-Antibody Complex; D002241:Carbohydrates; D000939:Epitopes; D007074:Immunoglobulin G; D009708:Nucleotidases; D015720:5'-Nucleotidase