A procedure to measure concanavalin-A binding with atomic spectroscopy and X-ray microanalysis.
The purpose of this study was to measure the binding of concanavalin A (conA) in minute regions of tissue by labelling the conA with iron dextran; then by measuring the bound iron per site by a procedure which uses atomic absorption spectrophotometry, X-ray microanalysis, and image analysis. The resulting data for a given region gamma are entered into the formula: (Formula: see text). The resulting quantity "iron at gamma" is directly proportional to conA binding in that region. For this study, three regions of rat renal cortex were compared: distal tubules, collecting ducts and blood vessels; glomeruli; and proximal tubules. Regional iron concentrations were: Combined region (distal tubules, etc.), 0.147 +/- 0.107 microgram/mg tissue; glomeruli, 0.199 +/- 0.087 microgram/mg tissue; and proximal tubules, 1.711 +/- 0.303 microgram/mg tissue.
Rosenquist
T H
TH
Huff
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TA
D000818:Animals; D003911:Dextrans; D004577:Electron Probe Microanalysis / Q000379:methods; D005260:Female; D007501:Iron; D007668:Kidney / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D011952:Receptors, Concanavalin A / Q000032:analysis*; D013054:Spectrophotometry, Atomic / Q000379:methods
D003911:Dextrans; D011952:Receptors, Concanavalin A; D007501:Iron