Effect of temperature on long-term survival of anucleate giant axons in crayfish and goldfish.
The effect of temperature on the electrophysiology and morphology of anucleate axons was examined following severance of crayfish medial giant axons and goldfish Mauthner axons from their respective cell bodies. Although anucleate segments of each giant axon exhibited long-term survival for weeks to months at 5-25 degrees C in crayfish and 10-30 degrees C in goldfish, the two axons differed in their survival characteristics. All measures of long-term survival in crayfish medial giant axons were independent of animal holding temperature, whereas all measures in Mauthner axons were dependent on holding temperature. Medial giant axons survived for at least 90 days in crayfish maintained at 5-25 degrees C in this and previous studies. Mauthner axons survived for over 5 months in goldfish maintained at 10 degrees C but survived for 1 month at 30 degrees C. Postoperative time had different effects on many single measures of long-term survival (axonal diameter, amplitude of action or resting potentials) in medial giant axons compared to Mauthner axons. For example, resting and action potentials in crayfish medial giant axons remained remarkably constant at all holding temperatures for 0-90 postoperative days. In contrast, resting and action potentials in goldfish Mauthner axons declined abruptly in the first 10-20 postoperative days followed by a slower decline at each holding temperature. We suggest that the mechanism of long-term survival is not necessarily the same in all anucleate axons.
Blundon
J A
JA
Department of Zoology, University of Texas, Austin 78712.
Sheller
R A
RA
Moehlenbruck
J W
JW
Bittner
G D
GD
D000818:Animals; D003400:Astacoidea / Q000502:physiology*; D001369:Axons / Q000502:physiology* / Q000648:ultrastructure; D001831:Body Temperature; D002470:Cell Survival; D003530:Cyprinidae / Q000502:physiology*; D004594:Electrophysiology; D006054:Goldfish / Q000502:physiology*; D009410:Nerve Degeneration; D013045:Species Specificity; D013119:Spinal Cord Injuries / Q000503:physiopathology; D013696:Temperature