Persistence of mumps virus in mouse L929 cells.
The characteristics of a persistent infection of L929 cells with mumps virus (MuV) is presented. The persistent infection (L-MuV cells) was regulated by interferon (IFN) produced endogenously and almost all the properties showed that the carrier culture was maintained by horizontal transmission of the virus. Small-plaque mutants, but not temperature-sensitive variants, were selected during the persistent infection. MuV released from L-MuV cells (MuV-pi) replicated efficiently in L929 cells, while infection of L929 cells with the original MuV-o resulted in an abortive infection. The efficient replication of MuV-pi in L929 cells can be explained by the findings that MuV-pi induced IFN more slowly and had lower susceptibility to IFN in L929 cells than MuV-o did. M protein was synthesized to a considerable degree in MuV-pi-infected cells, while it could not be detected in MuV-o-infected cells. By contrast, MuV-pi formed small plaques in Vero cell monolayers and the yield of MuV-pi in Vero cells was lower than that of MuV-o. M protein induced by MuV-pi decayed easily in Vero cells. M protein was considered to be a limiting factor for MuV replication in both cell lines.
Y
Y
Ito
M
M
Tsurudome
M
M
Hishiyama
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D007372:Interferons / Q000032:analysis; D007739:L Cells / Q000382:microbiology*; D051379:Mice; D008970:Molecular Weight; D009109:Mumps virus / Q000254:growth & development* / Q000276:immunology; D013696:Temperature; D013997:Time Factors; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
D000956:Antigens, Viral; D014764:Viral Proteins; D007372:Interferons