The sequence specificity of bleomycin-induced DNA damage in intact cells.
Bleomycin causes lesions to DNA in intact cells and in purified DNA under appropriate conditions. Using a middle repetitive DNA sequence called alpha-DNA as a target sequence, we have compared the sequence specificity of bleomycin-induced DNA cleavage in intact human cells and in purified human DNA. Bleomycin induces numerous cleavage sites in alpha-DNA which vary widely in intensity and give rise to a complex pattern of bands on a DNA sequencing gel. Unexpectedly, the intensity and position of bleomycin-induced DNA cleavage sites are very similar in intact human cells and in purified human DNA.
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D001483:Base Sequence; D001761:Bleomycin / Q000633:toxicity*; D002460:Cell Line; D004273:DNA, Neoplasm / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D007951:Leukemia, Myeloid; D010761:Phosphorus Radioisotopes
D004273:DNA, Neoplasm; D010761:Phosphorus Radioisotopes; D001761:Bleomycin