Excess of factor X antigen versus clotting counterpart in coumarin treated patients as determined by an Elisa method.
Factor X antigen was assayed by means of an Elisa (sandwich) method in 36 patients on long-term anticoagulant therapy. The average value observed was 31.4% +/- 12.2. In almost every instance the antigen level was higher than the clotting counterpart (14.4% +/- 4.5). In a few instances no major difference was noted between factor X antigen and factor X activity. The method correlated fairly well with other immunological methods (electroimmunoassay and Laser Nephelometer). Therefore, factor X Elisa method appears to be a suitable method for factor X antigen evaluation.
Girolami
A
A
Vicarioto
M A
MA
Ruzza
G
G
Vergolani
A
A
Boeri
G
G
D000941:Antigens / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005170:Factor X / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D014859:Warfarin / Q000627:therapeutic use*
D000941:Antigens; D014859:Warfarin; D005170:Factor X