Isolation and functional expression of pituitary peptidylglycine alpha-amidating enzyme mRNA. A variant lacking the transmembrane domain.
We demonstrate that, in rat pituitary, peptidylglycine alpha-amidating enzyme was encoded by at least 5 distinct mRNAs. Southern blot and ribonuclease protection analyses revealed that the mRNAs arose through alternative splicing. A variant lacking the transmembrane domain-coding sequence was a major mRNA species for the enzyme in the pituitary. When the cDNAs were expressed in COS-7 cells, the variant was the most efficient in producing a secretory form (37 kDA) of the enzyme.
Kato
I
I
Department of Biochemistry, Tohoku University School of Medicine, Miyagi, Japan.
Yonekura
H
H
Yamamoto
H
H
Okamoto
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D015971:Gene Expression Regulation, Enzymologic; D015723:Gene Library; D014644:Genetic Variation; D007700:Kinetics; D006899:Mixed Function Oxygenases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes; D010902:Pituitary Gland / Q000201:enzymology*; D010957:Plasmids; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains; D015183:Restriction Mapping; D014162:Transfection
D009097:Multienzyme Complexes; D012333:RNA, Messenger; D004247:DNA; D006899:Mixed Function Oxygenases; C035189:peptidylglycine monooxygenase