Angiotensin converting enzyme and substance P changes in blister fluid following afferent nerve stimulation in the rat.
We have examined the effect of electrical nerve stimulation on substance P and angiotensin converting enzyme activity in the interstitial fluid of rat skin using a blister model. Following sciatic nerve stimulation, blister fluid immunoreactive substance P (fmol/ml) was increased from 118 (unstimulated side, s.e.m. = 13, n = 15) to 197 (stimulated side, s.e.m. = 26, n = 15, P less than 0.0125, paired t-test, 14 d.f.). Angiotensin converting enzyme (ACE) activity (nmol HL/ml per h) was reduced in blister fluid from 26.5 (unstimulated side, s.e.m. = 2.4, n = 12) to 22.4 (stimulated side, s.e.m. = 1.4, P less than 0.05, paired t-test, 11 d.f.). Electrical stimulation of afferent nerves inhibits angiotensin converting enzyme activity in vivo. This may contribute to the process of neurogenic inflammation.
Jackson
B
B
White
D
D
Cubela
R
R
Helme
R
R
D000818:Animals; D001768:Blister / Q000378:metabolism*; D001826:Body Fluids / Q000378:metabolism; D004558:Electric Stimulation; D009475:Neurons, Afferent / Q000502:physiology*; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013373:Substance P / Q000378:metabolism*
D013373:Substance P; D007703:Peptidyl-Dipeptidase A