Typing and subtyping of herpes simplex isolates by monoclonal fluorescence.
The type specificity of 16 herpes simplex monoclonal antibodies was tested on 50 isolates previously typed by endonuclease restriction. A panel of antibodies was selected and used to type isolates from 50 primary and 50 recurrent genital infections. Sixty-four isolates from 18 patients followed up for six months were subtyped by using a larger panel of monoclonal antibodies and predominant HSV1 and HSV2 subtypes were demonstrated. In addition to confirming recurrences, four possible reinfections were identified. Type-specific differences in the location of viral antigens in infected cells were noted and a possible association of this finding with the type-specific differences in rates of recurrence of genital infections discussed.
Sutherland
S
S
Morgan
B
B
Mindel
A
A
Chan
W L
WL
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis / Q000276:immunology; D002478:Cells, Cultured; D004720:Endonucleases; D000939:Epitopes / Q000032:analysis*; D005347:Fibroblasts / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006558:Herpes Genitalis / Q000175:diagnosis / Q000453:epidemiology; D006801:Humans; D008168:Lung / Q000166:cytology / Q000196:embryology / Q000276:immunology; D012703:Serotyping; D018139:Simplexvirus / Q000032:analysis / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes; D004720:Endonucleases