Fluorescence in the tandem scanning microscope.
Our studies have shown that the fluorescence mode can be used to good effect in both tandem scanning microscopes (TSM: direct view confocal microscopes) as well as confocal scanning laser microscopes (CSLM). Applications are presented which show that the two great advantages of TSM are real-time viewing and real colour, which allow faster use and interpretation. CSLM are complementary, not competitive, being currently more sophisticated for low-level fluorescence work. This is equally possible with available TSM, but requires further development using CCD cameras and image-processing systems.
Boyde
A
A
Department of Anatomy and Developmental Biology, University College London, U.K.
Jones
S J
SJ
Taylor
M L
ML
Wolfe
L A
LA
Watson
T F
TF
D000818:Animals; D001846:Bone Development; D016025:Bone Transplantation / Q000502:physiology*; D001842:Bone and Bones / Q000378:metabolism*; D003731:Dental Caries / Q000473:pathology*; D003764:Dental Materials; D003793:Dental Restoration, Permanent; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence / Q000379:methods*; D010010:Osteoclasts / Q000648:ultrastructure; D011817:Rabbits; D051381:Rats; D012235:Rhodamines; D013752:Tetracycline
D003764:Dental Materials; D012235:Rhodamines; D013752:Tetracycline