Purification of Art v I, a relevant allergen of Artemisia vulgaris pollen.
An allergenic protein from Artemisia vulgaris pollen has been purified to homogeneity. Its molecular weight in native conditions is 47,000. The purified allergen, hereafter denominated Art v I, is a monomeric protein. It is a clinically relevant allergen since, at least, 70% of the individuals allergic to Artemisia vulgaris pollen have specific IgE in serum and in mast cells, demonstrated by ELISA and skin prick tests, respectively.
Alergia e Inmunología Abelló S.A., Madrid, Spain.
F
F
de la Hoz
F
F
Polo
J
J
Moscoso del Prado
J G
JG
Sellés
M
M
Lombardero
J
J
Carreira
D000485:Allergens / Q000276:immunology / Q000302:isolation & purification*; D015153:Blotting, Western; D011232:Chemical Precipitation; D006801:Humans; D015151:Immunoblotting; D007122:Immunoelectrophoresis; D007123:Immunoelectrophoresis, Two-Dimensional; D007073:Immunoglobulin E / Q000276:immunology; D007526:Isoelectric Point; D008970:Molecular Weight; D010940:Plant Proteins / Q000276:immunology* / Q000302:isolation & purification; D010944:Plants / Q000276:immunology*; D011058:Pollen / Q000276:immunology*
D000485:Allergens; D010940:Plant Proteins; D007073:Immunoglobulin E