Identification of histamine releasing factor(s) in the late phase of cutaneous IgE-mediated reactions.
We have shown that fluids collected from antigen-challenged skin blisters during the late phase reaction cause the release of substantial amounts of histamine (means = 42%, n = 14) from human basophils in vitro. Control fluids collected either during the immediate phase or from an unchallenged blister released less than or equal to 10% histamine from both basophils and lung mast cells. Late phase blister fluids induced low levels of histamine release from human lung cells (means = 11%, n = 4) that were slightly but not significantly greater than levels induced by control blister fluids. The characteristics of basophil release were similar to IgE-mediated stimuli in dose dependence, calcium and temperature requirements, and kinetics. The IgE dependence of the late phase blister fluid was demonstrated by desensitization of the basophils to anti-IgE, which obviated the response to anti-IgE and blister fluid but did not affect a non-IgE-mediated stimulus. Removal of the cell surface IgE with lactic acid also abolished the response to both anti-IgE and late phase blister fluid. Incubation of the "stripped" cells with serum containing IgE myeloma restored the response to anti-IgE but failed to affect response to late phase blister fluid. The characteristics of release obtained with this factor closely resemble those of an IgE-dependent histamine releasing factor from cultured macrophages previously described by our group.
J A
JA
Warner
M M
MM
Pienkowski
M
M
Plaut
P S
PS
Norman
L M
LM
Lichtenstein
D000485:Allergens / Q000008:administration & dosage; D000888:Antibodies, Anti-Idiotypic / Q000502:physiology; D001491:Basophils / Q000276:immunology / Q000378:metabolism; D001768:Blister / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D006636:Histamine Release; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology* / Q000378:metabolism; D007073:Immunoglobulin E / Q000276:immunology / Q000502:physiology*; D008222:Lymphokines / Q000502:physiology*; D029584:Prostatic Secretory Proteins; D012882:Skin Tests; D013997:Time Factors
D000485:Allergens; D000888:Antibodies, Anti-Idiotypic; D008222:Lymphokines; D029584:Prostatic Secretory Proteins; C026408:anti-IgE antibodies; C043076:beta-microseminoprotein; C013059:immunoglobulin-binding factors; D007073:Immunoglobulin E