Interferon affects nuclear proteins in cells of clinically sensitive chronic myelogenous leukemia patients.
Cytoplasmic protein extracts from chronic myelogenous leukemia (CML) cells contained an activity that altered the electrophoretic mobility of complexes formed between nuclear proteins and the transcriptional enhancers of interferon (IFN)-inducible genes. Exposure of CML cells to IFN-alpha diminished the effect of the CML cytoplasmic proteins on these nuclear protein-DNA complexes. The presence of clinical responsiveness to IFN-alpha correlated with the sensitivity to the IFN-induced change in the electrophoretic mobility of nuclear protein-DNA complexes. These data suggest that the action of IFN-alpha in CML may be linked to a pathway that can result in posttranslational modification of nuclear proteins.
Department of Hematology, University of Texas M.D. Anderson Cancer Center, Houston, TX.
O M
OM
Howard
M
M
Talpaz
H
H
Kantarjian
D
D
Seong
A
A
Wedrychowski
N
N
Paslidis
J
J
Hester
A
A
Cork
J
J
Turpin
G
G
Lopez-Berestein
D001483:Base Sequence; D001773:Blood Cells / Q000032:analysis / Q000166:cytology; D001853:Bone Marrow / Q000032:analysis; D001854:Bone Marrow Cells; D006801:Humans; D007279:Injections, Subcutaneous; D007370:Interferon Type I / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000473:pathology*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D009841:Oligonucleotides / Q000235:genetics; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics / Q000378:metabolism
D007370:Interferon Type I; D009687:Nuclear Proteins; D009841:Oligonucleotides; D014157:Transcription Factors