Interleukin 2 receptors are released by cells in vitro and in vivo. I. Detection of soluble IL 2 receptors in cell culture supernatants and in the serum of mice by an immunoradiometric assay.
An immunoradiometric assay (IRMA) detecting nanogram amounts of soluble mouse interleukin 2 receptors (IL 2R) is described. The IRMA is based on the finding that the two monoclonal antibodies AMT-13 and 7D4 react with two different epitopes of the mouse IL 2R molecule. As detected by IRMA, T lymphoblasts and IL 2R+ leukemic cells when incubated release IL 2R in soluble form into the culture medium. In the serum of mice inoculated with syngeneic IL 2R+ leukemic cells, growing as a solid tumor, IL 2R were detectable. The results raise the question whether circulating IL 2R are involved in down-regulation of immune responses.
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H
Osawa
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Josimovic-Alasevic
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T
Diamantstein
D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D000939:Epitopes; D007942:Leukemia, Experimental / Q000276:immunology / Q000378:metabolism; D008213:Lymphocyte Activation; D051379:Mice; D011863:Radioimmunoassay; D011971:Receptors, Immunologic / Q000378:metabolism*; D015375:Receptors, Interleukin-2; D012995:Solubility; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D011971:Receptors, Immunologic; D015375:Receptors, Interleukin-2