Analysis of RNA structure by ultraviolet crosslinking and denaturation gel electrophoresis.
Electrophoresis in polyacrylamide gels containing both formamide and urea is a high-resolution technique for the analysis of crosslinked RNA species. Combined with a specific crosslinking agent like uv irradiation, it allows a rapid fingerprint of structural differences between RNA forms. The technique reveals significant differences in the pattern of uv crosslinking of free Escherichia coli 16 S ribosomal RNA compared with the RNA in active or inactive 30 S subunits. Ultraviolet photocrosslinks seen only in the 30 S particle are likely to be tertiary structure contacts.
S A
SA
Quarless
C R
CR
Cantor
D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D011564:Furocoumarins; D009691:Nucleic Acid Denaturation; D012313:RNA / Q000032:analysis* / Q000528:radiation effects; D012335:RNA, Ribosomal / Q000032:analysis; D014466:Ultraviolet Rays
D003432:Cross-Linking Reagents; D011564:Furocoumarins; D012335:RNA, Ribosomal; D012313:RNA