Ia restriction of murine encephalitogenic T-cell lines in vitro and in vivo.
To clarify the functional role of the I region-associated (Ia) antigen in autoimmune central nervous system disorders, we generated long-term cultured lines of encephalitogenic T cells responsive to myelin basic protein from SJL strain mice (H-2s) and investigated genetic restriction in proliferative and encephalitogenic activities of the lines. These cell lines bear a Lyt 1+,2- phenotype, and show antigen-specific and I-As restricted proliferative responses in vitro. These lines induced full-blown experimental allergic encephalomyelitis (EAE) in immuno-compromised recipients carrying the I-As genotype. These data demonstrate that encephalitogenic T lymphocytes recognize the antigen in combination with the Ia antigen to induce EAE.
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Sakai
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Namikawa
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Kunishita
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Yoshimura
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Tabira
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D001921:Brain / Q000276:immunology; D002417:Cattle; D002455:Cell Division; D002460:Cell Line; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D000949:Histocompatibility Antigens Class II / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D004676:Myelin Basic Protein / Q000494:pharmacology; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology
D000949:Histocompatibility Antigens Class II; D004676:Myelin Basic Protein