The subunit structure of the insulin receptor and molecular interactions with major histocompatibility complex antigens.
Insulin receptors were labeled with 125I-photoreactive insulin (specifically labeling alpha-subunits) and by insulin-stimulated autophosphorylation (specifically labeling beta-subunits). The results show that the insulin receptor exists under different free and disulfide-linked combinations of alpha and beta subunits. Moreover, the insulin receptor is closely associated to class I antigens of the major histocompatibility complex to form a high molecular weight multi-molecular membrane complex.
M
M
Fehlmann
Y
Y
Chvatchko
D
D
Brandenburg
E
E
Van Obberghen
N
N
Brossette
D000255:Adenosine Triphosphate / Q000378:metabolism; D000345:Affinity Labels; D000818:Animals; D001386:Azides / Q000378:metabolism; D002460:Cell Line; D003432:Cross-Linking Reagents; D004220:Disulfides; D000939:Epitopes / Q000276:immunology; D006680:HLA Antigens / Q000276:immunology*; D006649:Histocompatibility Antigens / Q000276:immunology; D006801:Humans; D007163:Immunosorbent Techniques; D007328:Insulin / Q000031:analogs & derivatives / Q000378:metabolism; D046911:Macromolecular Substances; D008970:Molecular Weight; D010766:Phosphorylation; D010777:Photochemistry; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011972:Receptor, Insulin / Q000276:immunology*
D000345:Affinity Labels; D001386:Azides; D003432:Cross-Linking Reagents; D004220:Disulfides; D000939:Epitopes; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D007328:Insulin; D046911:Macromolecular Substances; C035459:insulin, (2-nitro-4-azidophenylacetyl)(B2)-des-Phe(B1)-; C036769:insulin, (2-nitro-4-azidophenylacetyl) (B29)-; D000255:Adenosine Triphosphate; D011505:Protein-Tyrosine Kinases; D011972:Receptor, Insulin