Androgen-repressed messages in the rat ventral prostate.
Poly (A)+ RNA from the prostates of both intact and castrated rats was translated in a message-dependent reticulocyte lysate, and the translation products were electrophoresed on SDS/polyacrylamide gels. Fluorography of these gels showed the expected disappearance, after castration, of the prostate steroid-binding proteins as well as a number of other androgen-dependent proteins. Two major (Mr 40,000 and 45,000) and several minor proteins appeared in the translation products of the castrated rat prostate RNA. Criss-cross liquid hybridization analysis between prostate poly (A+) RNA from intact and castrated rats also showed the disappearance of the abundant prostate steroid-binding protein sequences after castration and the synthesis of several new low to medium abundance sequences. Northern hybridization experiments demonstrated the presence of at least two, and possibly four androgen-repressed poly (A)+ RNA sequences. The most prominent of these, an RNA of 2,000 nucleotides, appeared within 2 days of castration, reaching a maximum on day 4 at a level approximately 400 times greater than the normal level. The other major sequence (a sequence of 1,000 nucleotides) appears after 4 days, reaching a peak between days 8 and 11. Sequences similar to these new RNAs could play an important role in the long-term resistance of prostatic cancer to hormone therapy in humans.
M L
ML
Montpetit
K R
KR
Lawless
M
M
Tenniswood
D000728:Androgens / Q000502:physiology*; D000818:Animals; D002474:Cell-Free System; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D009693:Nucleic Acid Hybridization; D009919:Orchiectomy; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D011467:Prostate / Q000378:metabolism* / Q000502:physiology; D011471:Prostatic Neoplasms / Q000378:metabolism; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D000728:Androgens; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA