Human tumor cells synthesize and secrete alpha-2-macroglobulin in vitro.
In previous studies we showed that human sarcoma and melanoma cell lines synthesize and secrete into culture medium a glycoprotein, migrating in urea sodium dodecyl sulfate-polyacrylamide gel electrophoresis at Mr 140,000. It is not detected in cultures of the corresponding normal cells. Conditioned medium of the melanoma cell line HMB-2, producing among the cell lines tested the largest amounts of this glycoprotein, has now been used as a source for purification of the protein. NH2-terminal amino-acid sequence determination of the purified glycoprotein showed that it is identical to human alpha 2-macroglobulin (alpha 2M). Rabbit antibodies raised against the glycoprotein specifically reacted in immunoblotting and immunodiffusion tests with alpha 2M present in human plasma. Likewise, these antibodies immunoprecipitated from the conditioned media of 35S-methionine-labelled melanoma and osteosarcoma cell lines the protein which had a molecular weight corresponding to alpha 2M. alpha 2M was also synthesized and secreted by 2 strains of fetal lung fibroblasts but not by fetal skin fibroblasts or adult skin fibroblasts autologous to the osteosarcoma cell line.
J
J
Bízik
A
A
Vaheri
O
O
Saksela
N
N
Kalkkinen
S
S
Meri
M
M
Grófóva
D002478:Cells, Cultured; D006023:Glycoproteins / Q000302:isolation & purification; D006801:Humans; D008545:Melanoma / Q000378:metabolism; D008970:Molecular Weight; D009369:Neoplasms / Q000378:metabolism*; D012509:Sarcoma / Q000378:metabolism; D000511:alpha-Macroglobulins / Q000276:immunology / Q000378:metabolism*
D006023:Glycoproteins; D000511:alpha-Macroglobulins