Treatment of Brucella canis and Brucella abortus in vitro and in vivo by stable plurilamellar vesicle-encapsulated aminoglycosides.
Stable plurilamellar vesicles (SPLVs) entrapping aminoglycosides were used to treat infections due to Brucella species (Brucella canis and Brucella abortus). SPLV-entrapped antibiotics effectively eliminated internalized B. canis in cultures of resident murine peritoneal macrophages and internalized B. abortus in cultures of resident guinea pig peritoneal macrophages. In vivo studies demonstrated that SPLV-entrapped aminoglycosides administered to B. canis-infected mice and B. abortus-infected guinea pigs effectively eliminated bacteria from infected organs. The dosage schedule used involved two intraperitoneal administrations of SPLV-entrapped aminoglycosides at three-day intervals. The results demonstrate the superiority of SPLV-entrapped aminoglycosides to free aminoglycosides in effecting elimination of facultative intracellular bacteria in vitro and in vivo. The use of SPLVs as a drug carrier has broad application to treatment of infections due to other organisms.
Fountain
M W
MW
Weiss
S J
SJ
Fountain
A G
AG
Shen
A
A
Lenk
R P
RP
D000617:Aminoglycosides / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002002:Brucella / Q000187:drug effects*; D002003:Brucella abortus / Q000187:drug effects*; D002006:Brucellosis / Q000188:drug therapy* / Q000382:microbiology; D002478:Cells, Cultured; D004096:Dihydrostreptomycin Sulfate / Q000494:pharmacology / Q000627:therapeutic use; D005260:Female; D005839:Gentamicins / Q000494:pharmacology / Q000627:therapeutic use; D006168:Guinea Pigs; D007612:Kanamycin / Q000494:pharmacology / Q000627:therapeutic use; D008081:Liposomes / Q000008:administration & dosage*; D008264:Macrophages / Q000382:microbiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000382:microbiology; D013307:Streptomycin / Q000494:pharmacology / Q000627:therapeutic use
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D005839:Gentamicins; D008081:Liposomes; D007612:Kanamycin; D004096:Dihydrostreptomycin Sulfate; D013307:Streptomycin