A peptide that inhibits the mitogenic stimulation of Swiss 3T3 cells by bombesin or vasopressin.
The synthetic peptide [D-Arg1,D-Pro2,D-Trp7,9,Leu1]substance P inhibits the stimulation of DNA synthesis induced in Swiss 3T3 cells by bombesin or vasopressin, but not that induced by a wide range of other growth factors and mitogens. The stimulation induced by 10 pM-3 nM-bombesin is inhibited by 1-30 microM-antagonist in a manner consistent with competition at the bombesin receptor. The inhibition by the antagonist of the stimulation induced by vasopressin suggests a previously unrecognized interaction of the antagonist with vasopressin receptors. The antagonist should be useful in studies of cell proliferation both in vivo and in vitro.
Corps
A N
AN
Rees
L H
LH
Brown
K D
KD
D000818:Animals; D001839:Bombesin / Q000494:pharmacology*; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D008934:Mitogens / Q000494:pharmacology*; D013373:Substance P / Q000031:analogs & derivatives* / Q000494:pharmacology; D014667:Vasopressins / Q000494:pharmacology*
D008934:Mitogens; D014667:Vasopressins; D013373:Substance P; C038073:substance P, Arg(1)-Pro(2)-Trp(7),(9)-LeuNH(2)(11)-; D004247:DNA; D001839:Bombesin