Measurement of N tau-methylhistamine concentrations in plasma and urine as a parameter for histamine release during anaphylactoid reactions.
N tau-methylhistamine concentrations in plasma and urine were determined using a newly developed simultaneous determination for histamine and N tau-methylhistamine, based on isotope dilution mass fragmentography. Three groups of patients were investigated: patients receiving intravenously-administered iodamide for excretory urography, patients receiving a wasp-sting challenge, and patients treated with an intravenously-administered muscle relaxant. In all patients showing a distinct systemic anaphylactic or anaphylactoid reaction histamine and N tau-methylhistamine concentrations were found to be elevated. From the results of this study it can be concluded that N tau-methylhistamine in plasma and urine is a good parameter for histamine release, and that the determination of this histamine metabolite are less hampered by possible artefacts (due to basophil disrupture, a very short half-life time or bacterial production) than determinations of histamine itself.
Keyzer
J J
JJ
Breukelman
H
H
Wolthers
B G
BG
Richardson
F J
FJ
de Monchy
J G
JG
D000707:Anaphylaxis / Q000378:metabolism*; D001826:Body Fluids / Q000032:analysis*; D005260:Female; D006632:Histamine / Q000032:analysis; D006636:Histamine Release; D006801:Humans; D008297:Male; D008761:Methylhistamines / Q000032:analysis* / Q000097:blood / Q000652:urine
D008761:Methylhistamines; D006632:Histamine