The localization and characterization of substance P and substance K in striatonigral neurons.
Specific substance P and substance K radioimmunoassays coupled to high-performance liquid chromatography were used to characterize striatal and nigral tachykinin immunoreactivity. Using these assays, authentic substance P and substance K accounted for nearly all substance P and substance K immunoreactivity, respectively. A series of coronal knife cuts of the striatum caused parallel depletions in nigral substance P and substance K, consistent with the possible colocalization of these tachykinins in striatonigral neurons.
Lee
J M
JM
McLean
S
S
Maggio
J E
JE
Zamir
N
N
Roth
R H
RH
Eskay
R L
RL
Bannon
M J
MJ
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003342:Corpus Striatum / Q000032:analysis*; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis*; D015288:Neurokinin A; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013373:Substance P / Q000032:analysis*; D013378:Substantia Nigra / Q000032:analysis*
D009419:Nerve Tissue Proteins; D013373:Substance P; D015288:Neurokinin A