Comparative ultrastructure of cancerous and normal human breast cells: demonstration of cytoskeleton by TEM.
A technique for the study of the cytoskeleton by transmission electron microscopy is described. It preserves cytoplasmic and nuclear ultrastructural details as well as cytoskeletal elements enabling a correlated study. There are no significant differences in organization and distribution of intermediate filaments between cancerous and normal breast cells. Close association between cytokeratin filaments and cell structures has been established from stereotilted images. Cytokeratin filaments in contact with cell structures are in the form of bundles; in the free 'space' of cytoplasm meshworks are organized. The intermediate filament system could possibly be organized for participation in diverse functions in a cell.
K B
KB
Ahluwalia
L K
LK
Sharma
D001940:Breast / Q000648:ultrastructure*; D001943:Breast Neoplasms / Q000648:ultrastructure*; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000648:ultrastructure*; D003599:Cytoskeleton / Q000648:ultrastructure*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007382:Intermediate Filaments / Q000648:ultrastructure*; D007633:Keratins / Q000032:analysis; D008854:Microscopy, Electron; D013696:Temperature
D007633:Keratins