Degradation of substance P by the neuroblastoma cells and their membrane.
Cells of the N-18 line of mouse neuroblastoma and their membrane degrade substance P added exogenously. The degradation by the cells and their membrane, examined by high-performance liquid chromatography, is strongly inhibited by EDTA but scarcely inhibited by captopril and phosphoramidon. Gly-Leu-Met-NH2 is the major cleavage product among C-terminal fragments of substance P in both cases. Thus, the degradation of substance P by the neuroblastoma cells and their membrane seems to take place mainly through the hydrolysis between Phe8-Gly9 by EDTA-sensitive protease(s).
S
S
Endo
H
H
Yokosawa
S
S
Ishii
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002216:Captopril / Q000494:pharmacology; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004492:Edetic Acid / Q000494:pharmacology; D006020:Glycopeptides / Q000494:pharmacology; D051379:Mice; D009447:Neuroblastoma / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D010446:Peptide Fragments / Q000032:analysis; D013373:Substance P / Q000378:metabolism*; D013570:Synaptic Membranes / Q000378:metabolism
D000596:Amino Acids; D006020:Glycopeptides; D010446:Peptide Fragments; D013373:Substance P; D004492:Edetic Acid; D002216:Captopril; C008890:phosphoramidon