Partial copurification of scrapie-associated fibrils and scrapie infectivity.
The association between scrapie infectivity and scrapie-associated fibrils (SAF) during a partial purification procedure for infectivity was investigated. Scrapie infectivity and SAF can be separated from most membrane components by subcellular fractionation of infected mouse brain to obtain a synaptosomal fraction, followed by detergent treatment and density gradient centrifugation. After different detergent treatments, with either octyl glucoside or sodium N-lauroyl sarcosinate, SAF showed differing sedimentation characteristics but nevertheless cosedimented with scrapie infectivity in both cases. Copurification under two different conditions provides more evidence that SAF may be a form of the infectious agent of scrapie.
Somerville
R A
RA
Merz
P A
PA
Carp
R I
RI
D000818:Animals; D001921:Brain / Q000382:microbiology; D001923:Brain Chemistry; D003902:Detergents / Q000494:pharmacology; D005960:Glucosides / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000302:isolation & purification*; D018622:PrP 27-30 Protein; D011328:Prions / Q000472:pathogenicity*; D012521:Sarcosine / Q000031:analogs & derivatives / Q000494:pharmacology; D013570:Synaptic Membranes / Q000032:analysis; D013574:Synaptosomes / Q000032:analysis / Q000187:drug effects / Q000382:microbiology
D003902:Detergents; D005960:Glucosides; D009419:Nerve Tissue Proteins; D011328:Prions; D018622:PrP 27-30 Protein; C018619:octyl-beta-D-glucoside; C025231:sarkosyl; D012521:Sarcosine