Human inter-alpha-trypsin-inhibitor: characterization and partial nucleotide sequencing of a light chain-encoding cDNA.
A synthetic Inter-alpha-Trypsin-Inhibitor (ITI) -specific oligonucleotide probe was used to isolate a clone from a human liver cDNA library. The amino-acid sequence deduced from partial nucleotide sequencing of the corresponding cDNA insert perfectly matched a known ITI sequence, apart from an as yet unreported C-terminal dipeptide. Hybridization on Northern blots evidenced that this cDNA insert originated from an ITI light chain-encoding mRNA whose size was estimated to be 1 300 bases.
Bourguignon
J
J
Diarra-Mehrpour
M
M
Sesboüé
R
R
Frain
M
M
Sala-Trepat
J M
JM
Martin
J P
JP
Salier
J P
JP
D000328:Adult; D000510:Alpha-Globulins / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D006801:Humans; D008099:Liver / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D010215:Papio; D010446:Peptide Fragments / Q000235:genetics; D012333:RNA, Messenger
D000510:Alpha-Globulins; D010446:Peptide Fragments; D012333:RNA, Messenger; C011877:inter-alpha-inhibitor; D004247:DNA