Properties of voltage-sensitive sodium channels in neuroblastoma cells grown in chemically defined and serum-supplemented media.
The saxitoxin binding properties and sodium currents were compared in N18 neuroblastoma cells grown in serum-supplemented and chemically defined serum-free media. There was no alteration in either maximal binding capacity or receptor affinity and the kinetics or voltage dependency of sodium channel activation and inactivation. It is concluded that sodium channels expressed by neuroblastoma cells grown in the chemically defined media were functionally identical to those in cells grown in serum-supplemented medium.
Willow
M
M
Gonoi
T
T
D000818:Animals; D002999:Clone Cells; D003470:Culture Media; D007106:Immune Sera / Q000494:pharmacology; D007473:Ion Channels / Q000502:physiology*; D008564:Membrane Potentials; D051379:Mice; D009447:Neuroblastoma; D012530:Saxitoxin / Q000378:metabolism; D012964:Sodium / Q000378:metabolism*
D003470:Culture Media; D007106:Immune Sera; D007473:Ion Channels; D012530:Saxitoxin; D012964:Sodium