The T cell receptor: the alpha and beta chains define idiotype, and antigen and MHC specificity.
Three independent T cell hybridomas were isolated that have identical specificities for antigen and products of the major histocompatibility complex (MHC). All three react with the same clone-specific antireceptor antibody, and Southern blots show all three contain the same rearranged alpha and beta genes. Variants of one of these hybridomas, DO-11.10, were isolated that had lost the ability to respond to antigen plus MHC. These proved to have lost the DO-11.10-specific alpha or beta genes or both. Fusion of alpha-loss variants to beta-loss variants restored reactivity. These results indicate that the specific recognition of antigen plus MHC is determined solely by the alpha/beta-containing T cell receptor.
J
J
Yagüe
J
J
White
C
C
Coleclough
J
J
Kappler
E
E
Palmer
P
P
Marrack
D000818:Animals; D000939:Epitopes; D005796:Genes; D005816:Genetic Complementation Test; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006825:Hybridomas; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D008285:Major Histocompatibility Complex; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000939:Epitopes; D000949:Histocompatibility Antigens Class II; D007130:Immunoglobulin Idiotypes; D011948:Receptors, Antigen, T-Cell; D010047:Ovalbumin