Gossypol modulation of mouse heart and liver lactate dehydrogenase isoenzymes as a function of gender.
Acute treatment with gossypol produced both tissue specific and sex-dependent alterations of M and H subunits of mouse lactate dehydrogenase. Gossypol induced female mouse heart cytoplasma H subunit and inhibited both enzyme subunits in the male mouse. Gossypol induced mouse liver mitochondrial lactate dehydrogenase subunit M in both sexes 50 hrs after acute drug administration while was ineffective on the testicular enzyme. Induction of hepatic lactate dehydrogenase may enhance NADH:NAD ratio, resulting in alteration of cellular redox status and reduction of NAD utilization by other dehydrogenases. The gossypol-mediated sex-dependent changes of heart lactate dehydrogenase isoenzymes may be of value in the prediction of certain aspects of gossypol toxicity.
Texas Tech University Health Sciences Center, School of Medicine, Lubbock 79430.
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D000818:Animals; D003593:Cytoplasm / Q000187:drug effects / Q000201:enzymology; D005260:Female; D006072:Gossypol / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D051379:Mice; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology*; D012737:Sex Factors; D013737:Testis / Q000187:drug effects / Q000201:enzymology
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D006072:Gossypol