Monoclonal antibodies against the presynaptic calcium channel antagonist omega-conotoxin GVI A from cone snail poison.
Monoclonal antibodies have been prepared against omega-conotoxin GVI A, a peptide isolated from marine snails of the genus Conus (Conus geographus and Conus magus). This toxin is a blocker of select presynaptic Ca2+ channels in the central nervous system. Antigenic omega-conotoxin GVI A was synthesized as a covalent conjugate with bovine serum albumin and injected s.c. An ELISA assay combined with a competitive inhibition assay was used to select and characterize monoclonal antibodies able to recognize and bind the free toxin. Several of the antibodies were found to block omega-conotoxin GVI A inhibition of 45Ca transport into rat brain synaptosomes and to block omega-conotoxin GVI A binding to membranes from the same preparation. The antibodies recognize native, synthetic toxin, and are useful for analysis of toxin in biological fluids.
Laboratory of Cell Biology and Genetics, NIDDK, National Institutes of Health, Bethesda, MD.
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D
Tombaccini
O M
OM
Adeyemo
H B
HB
Pollard
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G
Feuerstein
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000494:pharmacology; D000941:Antigens / Q000276:immunology; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism; D002121:Calcium Channel Blockers; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006054:Goldfish; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D008978:Mollusk Venoms / Q000032:analysis / Q000276:immunology* / Q000378:metabolism; D051381:Rats; D012710:Serum Albumin, Bovine / Q000276:immunology; D013574:Synaptosomes / Q000378:metabolism; D020866:omega-Conotoxin GVIA
D000911:Antibodies, Monoclonal; D000941:Antigens; D002121:Calcium Channel Blockers; D008978:Mollusk Venoms; D012710:Serum Albumin, Bovine; D020866:omega-Conotoxin GVIA