Multiple forms of angiotensin-converting enzyme in human tissues and fluids.
Angiotensin-converting enzyme (EC 3.4.15.1) exhibits distinctive tissue differences in electrophoretic behaviour. Human tissue homogenates and biological fluids were subjected to agar gel and polyacrylamide gel electrophoresis. The gels were cut into slices and enzymatic activity detected by a specific colorimetric reaction. The technique allows localization of the enzyme in the electrophoretogram and gives an estimate of the total enzymatic activity of a tissue or biological fluid. Several tissues were found to show multiple forms of angiotensin-converting enzyme. The specificity of the assay was determined by running the samples after the addition of captopril and by adding proteinase inhibitors to the incubation mixture.
van Sande
M
M
Scharpé
S L
SL
Neels
H M
HM
Kasahara
Y
Y
D001826:Body Fluids / Q000201:enzymology*; D004064:Digestive System / Q000201:enzymology; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007202:Indicators and Reagents; D007527:Isoenzymes / Q000032:analysis*; D008297:Male; D007703:Peptidyl-Dipeptidase A / Q000032:analysis*; D011470:Prostatic Hyperplasia / Q000201:enzymology; D011471:Prostatic Neoplasms / Q000201:enzymology
D007202:Indicators and Reagents; D007527:Isoenzymes; D007703:Peptidyl-Dipeptidase A