Isolation of specific antigens from Angiostrongylus cantonensis. 1. Preparative flatbed isoelectric focusing.
Electrophoresis on SDS gel and analytical isoelectric focusing showed that a crude extract of Angiostrongylus cantonensis consisted of at least 40 protein components with molecular weights ranging from 13 000-70 000 and isoelectric points of pI values ranging from 3.7-10.0. Crossed-immunoelectrophoresis with a hyperimmune antiserum to A. cantonensis showed at least 40 different antigenic components in the crude worm extract which were cross-reactive with those of Ascaris suum, Metastrongylus apri and Toxocara canis. Using preparative isoelectric focusing, the somatic worm preparation was divided into 13 equal fractions, of which 3, 4 and 5, with pI values of 3.7, 4.0 and 4.45 respectively, were later shown by immunoelectrophoretic techniques and enzyme-linked immunosorbent assay to contain antigens specific to A. cantonensis.
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D000801:Angiostrongylus / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000032:analysis; D000947:Antigens, Helminth / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D001200:Ascaris / Q000276:immunology; D003378:Counterimmunoelectrophoresis; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D005260:Female; D005455:Fluorescent Antibody Technique; D007114:Immunization; D007122:Immunoelectrophoresis; D007525:Isoelectric Focusing / Q000379:methods; D008297:Male; D008681:Metastrongyloidea / Q000276:immunology*; D010124:Oxyuroidea / Q000276:immunology; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D014119:Toxocara / Q000276:immunology
D000906:Antibodies; D000947:Antigens, Helminth; D000939:Epitopes