[Experimental study of cellular response on the intraocular lens surface in terms of the presence and distribution of fibronectin].
An immunohistochemical study was performed to observe cellular proliferation on the surface of implanted intraocular lenses (IOLs) in rabbit eyes. Rabbits were killed 3-28 days postoperatively. The removed IOLs were examined by an immunoperoxidase staining method using anti-fibronectin antibodies. The immunoreactivity for fibronectin was detected in macrophages and multinucleated giant cells attached to the IOL surface. Prominent stains were observed in these cells 1 week after the operation, and staining for fibronectin was less intense in the specimens 2 and 4 weeks after implantation. Fibronectin is produced by macrophages and multinucleated giant cells on the IOL surface, and might play important role in cellular adhesion and motility. Fibronectin immunoreactivity decreased with time and it may be related to cellular activity.
Department of Ophthalmology, Wakayama Medical College, Japan.
R
R
Kanagawa
S
S
Saika
K
K
Miyamoto
S
S
Ohmi
M
M
Tamura
T
T
Nakao
D000818:Animals; D005353:Fibronectins / Q000378:metabolism*; D007150:Immunohistochemistry; D007910:Lenses, Intraocular; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism; D008297:Male; D011817:Rabbits
D005353:Fibronectins