Appearance of M-band attached MM-creatine kinase in differentiating chicken heart cells after injection of M-type isoprotein or poly (A+)-RNA enriched for M-type creatine kinase message.
Myofibrils of chicken heart cells do not contain the electron-dense material of the so-called m-bridges which transverse the sarcomere in the M-region. It has been shown that the M-isoform of creatine kinase (MM-CK) which is mainly responsible for the m-bridge material is not expressed during differentiation of chicken heart cells. No transition from the embryonic BB-CK to the muscle-specific MM-CK takes place in chicken heart, thus no MM-CK is available for m-bridge formation. Here we report on chicken heart cells microinjected with either MM-CK protein or with poly(A+)RNA enriched for M-CK message. In both cases appearance of MM-CK within the M-band of heart myofibrils could be observed by immunofluorescence, indicating translation of the injected message as well as specific binding of the translation product to the M-band of myofibrils. The M-band protein myomesin which is regularly found in heart myofibrils served as specific marker for assembled myofibrils in double immunofluorescence experiments.
B
B
Schäfer
J C
JC
Perriard
H M
HM
Eppenberger
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D002642:Chick Embryo; D003402:Creatine Kinase / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D007527:Isoenzymes; D008845:Microinjections; D009206:Myocardium / Q000166:cytology* / Q000201:enzymology; D009210:Myofibrils / Q000378:metabolism*; D011061:Poly A / Q000378:metabolism*; D014176:Protein Biosynthesis; D012313:RNA / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism
D007527:Isoenzymes; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D003402:Creatine Kinase