Human prostate tissue antigens defined by murine monoclonal antibodies.
BALB/c mice were hyperimmunized against a membrane preparation derived from a pool of transurethral resection specimens which included three benign prostatic hyperplasia and one prostate adenocarcinoma tissue samples. The activated lymphocytes were fused with the NS-1 mouse myeloma cell line, and supernatants from immunogen-reactive hybridomas were screened for antibody binding activity using a solid-phase radioimmunoassay against the Calu-1 human lung adenocarcinoma cell line and several membrane preparations derived from various normal human tissues. Hybridoma cultures secreting antibodies which did not appear cross-reactive were doubly cloned by limiting dilution and screened against a large panel of membrane preparations derived from normal prostate, benign prostatic hyperplasia, and prostate adenocarcinoma tissues as well as samples obtained from a variety of normal human tissues. The monoclonal antibodies were also evaluated against 24 normal, virally transformed, and malignant human cell lines. Two monoclonal antibodies were isolated which demonstrated a restricted binding activity to prostate antigens and were not widely cross-reactive with nonprostate normal tissues or cell lines. These antibodies were designated TURP-27 (IgG3, k) and TURP-73 (IgG2a, k). Both of these monoclonal antibodies were reactive against formalin-fixed, paraffin-embedded tissues in the immunoperoxidase assay and were subsequently tested against a variety of normal, hyperplastic, and malignant human tissues. These studies indicated that TURP-27 may be directed against a new prostate organ-associated marker and that TURP-73 is directed against an antigen expressed on prostate and a limited number of other tissues.
Starling
J J
JJ
Sieg
S M
SM
Beckett
M L
ML
Wirth
P R
PR
Wahab
Z
Z
Schellhammer
P F
PF
Ladaga
L E
LE
Poleskic
S
S
Wright
G L
GL
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001940:Breast / Q000276:immunology; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D003106:Colon / Q000276:immunology; D003429:Cross Reactions; D006801:Humans; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000276:immunology; D008297:Male; D011467:Prostate / Q000276:immunology*; D011470:Prostatic Hyperplasia / Q000276:immunology*; D011471:Prostatic Neoplasms / Q000276:immunology*; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal