Monoclonal T cell responses to two epitopes on a single immunogen controlled by two distinct genes.
The fine specificities of immune T cells were studied in a system in which the response to the antigen can involve two immune response (Ir) genes and two epitopes on a single synthetic polypeptide immunogen. The (BALB/c X SJL)F1 (H-2d X H-2s) mice can respond to the random terpolymer poly(Glu55, Lys36, Phe9) (GLPhe) through the H-2d-linked Ir gene (Ir-d) or through the complementing Ir gene (Ir-dxs), which controls the immune response to poly(Glu, Phe), epitopes that are present in GLPhe. Nine groups of monoclonal T cells were obtained from (H-2d X H-2s)F1 mice immunized with GLPhe. These groups were delineated by the differences in major histocompatibility (MHC) restriction on antigen-presenting cells (APC) and the cross-reactions with GPhe or GLT. A unique T cell line was discovered that can react to the three polymers (GLPhe, GLT, GPhe) even though GLT and GPhe immune T cells do not normally show reciprocal cross-reactions. The monoclonal T cells retain helper activities in the Mishell-Dutton culture. Although the activation of T cells is antigen specific and MHC restricted, the subsequent B cell response is nonspecific.
C H
CH
Lai
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Babu
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HA
Turchin
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PH
Maurer
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D002999:Clone Cells / Q000276:immunology; D000939:Epitopes; D005802:Genes, MHC Class II; D006241:Haptens / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D008285:Major Histocompatibility Complex; D051379:Mice; D010455:Peptides / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D000939:Epitopes; D006241:Haptens; D010455:Peptides; C027143:poly(Glu(56)-Lys(35)-Phe(9))n