Evidence that large synaptic vesicles containing substance P and small synaptic vesicles have a surface antigen in common in rat.
Synaptic vesicles were purified from rat brain synaptosomes by osmotic lysis and chromatography on CPG-3000 controlled-pore glass beads. Large (approximately 100 nm) synaptic vesicles containing the peptide substance P (SP) were shown to be immunoprecipitated by a monoclonal antibody previously shown by W.D. Matthew, L. Tsavaler and L.F. Reichardt (J. Cell Biol., 91 (1981) 257-269) to selectively immunoprecipitate small (approximately 50 nm) synaptic vesicles from brain. Precipitation of SP-containing vesicles showed a saturable dependence on antibody concentration. These findings constitute direct, immunochemical evidence that large, peptidergic synaptic vesicles and small synaptic vesicles from brain have a surface antigen in common.
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D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D001921:Brain / Q000276:immunology*; D051381:Rats; D013373:Substance P / Q000032:analysis*; D013572:Synaptic Vesicles / Q000032:analysis / Q000276:immunology* / Q000648:ultrastructure
D000954:Antigens, Surface; D013373:Substance P