[Use of the methods of blood protein fractionation for increasing the activity of natural anti-A and anti-B antibodies].
Effectiveness has been shown of human blood serum fractionation with polyethylene glycol for isolation of anti-A and anti-B isohemagglutinins from stocks with low antibody activity, inadequate for the production of AB0-typing standards. An optimal diluent of fractions containing antibodies of the AB0 system has been selected. The reagents prepared did not differ, by their activity and specificity, from the standard ones produced from the whole sera.
K N
KN
Klimova
M A
MA
Matveeva
N V
NV
Mineeva
D000017:ABO Blood-Group System / Q000276:immunology*; D001798:Blood Proteins / Q000032:analysis*; D006388:Hemagglutinins / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology / Q000302:isolation & purification*; D007202:Indicators and Reagents; D007518:Isoantibodies / Q000276:immunology / Q000302:isolation & purification*; D011092:Polyethylene Glycols / Q000494:pharmacology; D012699:Serology / Q000379:methods*
D000017:ABO Blood-Group System; D001798:Blood Proteins; D006388:Hemagglutinins; D007075:Immunoglobulin M; D007202:Indicators and Reagents; D007518:Isoantibodies; D011092:Polyethylene Glycols
Ispol'zovanie metodov fraktsionirovaniia belkov krovi dlia povysheniia aktivnosti estestvennykh anti-A, anti-B antitel.