Maturation of cholecystokinin receptors in pancreatic acini of rats.
Amylase secretion by fetal and newborn rat pancreata was shown not to respond to cholecystokinin (CCK) stimulation. Binding of 125I-Bolton-Hunter (125I-BH)-CCK-8 to dispersed pancreatic acini from rats at various perinatal ages was measured and compared with adults. Binding occurred maximally at 30 min at 37 degrees C at all ages. Maximal binding increased with age. The capacity and affinity of the CCK-receptor binding system was analyzed by Scatchard's method. The results revealed that the capacity of the high-affinity component gradually increases with age, whereas that of the low-affinity component remains relatively constant. Amylase secretion due to CCK stimulation also increases with age, suggesting that the lack of responsiveness of pancreata of pups to secretagogue is due to a low binding capacity of the high-affinity component of the receptors.
Y K
YK
Leung
P C
PC
Lee
E
E
Lebenthal
D000375:Aging; D000681:Amylases / Q000378:metabolism; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D007700:Kinetics; D010179:Pancreas / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011949:Receptors, Cholecystokinin; D012844:Sincalide / Q000378:metabolism / Q000494:pharmacology
D011956:Receptors, Cell Surface; D011949:Receptors, Cholecystokinin; D000001:Calcimycin; D003994:Bucladesine; D002217:Carbachol; D000681:Amylases; D012844:Sincalide