Virus-specific early antigen expressed in the nucleus of cowpox virus-infected cells.
Two monoclonal antibodies (MAbs) were obtained from BALB/c mice immunized with cowpox virus (CPV)-infected cells cultured in the presence of cytosine-1-beta-D-arabinofuranosyl-HCl (Ara C). In the immunofluorescence test, the specific antigen reacting with these MAbs was restricted almost entirely to the nucleus in CPV-infected cells in the presence of Ara C at any time. On the other hand, in CPV-infected cells in the absence of Ara C, the antigen was first detectable within the nucleus 2 h after infection and migrated to the cytoplasm as the infection proceeded. On immunoblotting, only one component with an Mr of 27K was detected 2 to 24 h after infection of cells with CPV in either the presence or absence of Ara C. The antigen was also detected in vaccinia virus-infected cells, but not in any mock-infected cells nor in any virions purified from infected cells. These results suggest that the antigen reacting with the MAbs is virus-specific and a non-structural early antigen.
Department of Microbiology, Wakayama Medical College, Japan.
N
N
Kitamoto
K
K
Hiroi
K
K
Miyamoto
T
T
Tanaka
H
H
Miyamoto
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis*; D015153:Blotting, Western; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology*; D015608:Cowpox virus / Q000276:immunology*; D003561:Cytarabine / Q000494:pharmacology; D003593:Cytoplasm / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D006825:Hybridomas; D007739:L Cells; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D003561:Cytarabine