Analysis of T-cell receptor gene rearrangement and expression in human natural killer clones.
A series of clones of human natural killer (NK) cells was characterized with respect to expression of the Ti alpha and Ti beta genes of the T-cell receptor. T11+T3+ NK clones contained Ti alpha and Ti beta RNA transcripts and expressed disulfide-linked heterodimers, demonstrating the presence of a functional T-cell receptor. In contrast, T11+T3- NK clones expressed only 1.0-kilobase truncated Ti beta transcripts, without a Ti alpha transcript and no detectable surface Ti protein. Since previous studies demonstrated that Ti beta gene activation precedes Ti alpha gene activation in thymic ontogeny, the T11+T3- NK cells appear to be derived from T-lineage precursors.
Ritz
J
J
Campen
T J
TJ
Schmidt
R E
RE
Royer
H D
HD
Hercend
T
T
Hussey
R E
RE
Reinherz
E L
EL
D002999:Clone Cells / Q000032:analysis; D003602:Cytotoxicity, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D006801:Humans; D007694:Killer Cells, Natural / Q000032:analysis*; D010641:Phenotype; D012313:RNA / Q000032:analysis; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D014158:Transcription, Genetic; D015533:Transcriptional Activation
D011948:Receptors, Antigen, T-Cell; D012313:RNA