Two stages of the differentiation of HL-60 cells induced by 1 alpha,25 dihydroxyvitamin D3; commitment by 1 alpha,25 dihydroxyvitamin D3 and promotion by DMSO.
The differentiation of HL-60 cells induced by 1 alpha,25 dihydroxyvitamin D3 was found to be separated into two stages, i.e. commitment and promotion. Most of the HL-60 cells were committed to monocyte/macrophage lineage by pretreatment with 1 alpha,25 dihydroxyvitamin D3 (5-50 ng/ml) for 18-24 hr. The promotion in the second stage was inducer and lineage independent; treatment with 1.25% DMSO for 2 or 3 days promoted the differentiation of the committed HL-60 cells by 1 alpha,25 dihydroxyvitamin D3 into monocyte/macrophage lineage, but not granulocyte lineage.
Momoi
T
T
Senoo
H
H
Yoshikura
H
H
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D002117:Calcitriol / Q000494:pharmacology* / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D004121:Dimethyl Sulfoxide / Q000494:pharmacology* / Q000502:physiology; D000939:Epitopes / Q000032:analysis; D004950:Esterases / Q000032:analysis; D006098:Granulocytes / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000276:immunology; D006801:Humans; D015470:Leukemia, Myeloid, Acute; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000276:immunology; D013997:Time Factors; D014212:Tretinoin / Q000494:pharmacology / Q000502:physiology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000939:Epitopes; D014212:Tretinoin; D004950:Esterases; D002117:Calcitriol; D004121:Dimethyl Sulfoxide