A comparison of silver staining methods for detecting proteins in ultrathin polyacrylamide gels on support film after isoelectric focusing.
The application of silver staining methods to the detection of proteins on ultrathin isoelectric focusing gel systems requires the optimization of many steps in the procedure in order to obtain reproducible staining of proteins with acceptable levels of background. Three different methods which have been reported for detecting proteins by silver staining in sodium dodecyl sulfate-polyacrylamide gel systems were investigated. A major problem with staining ultrathin isoelectric focusing gels was found to be surface staining that was associated with gels cast on support films. A modification of the method of Poehling and Neuhoff (H.-M. Poehling and V. Neuhoff, 1981, Electrophoresis 2, 141-147) was found to give the best results.
Butcher
L A
LA
Tomkins
J K
JK
D004396:Coloring Agents; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D011506:Proteins / Q000032:analysis*; D012394:Rosaniline Dyes; D012834:Silver; D013194:Staining and Labeling
D004396:Coloring Agents; D011506:Proteins; D012394:Rosaniline Dyes; D012834:Silver; C024757:Coomassie brilliant blue R