Monitoring of circadian fluctuations of N-acetylserotonin in the rat pineal body by differential pulse voltammetry.
Circadian fluctuations of the electrochemical signal appearing at +270 mV (peak 3) recorded from the pineal body of freely moving rats were first monitored for 24 hours using the in vivo voltammetry technique. The peak 3 height increased after injection of pargyline and S-adenosyl-L-homocysteine but decreased after injection of NSD-1015, while probenecid did not cause any change. Under a 12/12 hours light-dark cycle, the peak 3 height represented circadian fluctuations, similar to those of N-acetyltransferase activity, which were higher in the dark than in the light period. These data suggest that the compound responsible for peak 3 in the pineal body is essentially due to extracellular N-acetylserotonin.
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Ikeda
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Nagatsu
D000818:Animals; D002940:Circadian Rhythm; D004563:Electrochemistry; D006834:Hydrazines / Q000494:pharmacology; D006897:Hydroxyindoleacetic Acid / Q000032:analysis; D008027:Light; D008297:Male; D010293:Pargyline / Q000494:pharmacology; D010870:Pineal Gland / Q000032:analysis*; D011339:Probenecid / Q000494:pharmacology; D051381:Rats; D012435:S-Adenosylhomocysteine / Q000494:pharmacology; D012701:Serotonin / Q000031:analogs & derivatives* / Q000032:analysis
D006834:Hydrazines; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D012435:S-Adenosylhomocysteine; D010293:Pargyline; C004297:3-hydroxybenzylhydrazine; C006389:N-acetylserotonin; D011339:Probenecid