Transfection of murine LMTK- cells with purified HLA class I genes. VII. Association of allele- and locus-specific serological reactivities with respectively the first and second domains of the HLA-B7 molecule.
The individual contributions of the first two external domains of the HLA-B7 heavy chain to the expression of allele-specific (B7) and locus-specific (B and C) antigenic determinants were investigated using hybrid class I genes. Hybrid genes were constructed in vitro by exon shuffling between the parent genes HLA-B7, HLA-Cw3, HLA-A3, and H-2Kd, and their expression was monitored following transfection into mouse L cells. The results show that most allele-specific antigenic determinants are associated with the first external domain of the B7 heavy chain, whereas all the locus-specific antigenic determinants tested map to the second external domain.
Sodoyer
R
R
Kahn-Perles
B
B
Strachan
T
T
Sire
J
J
Santoni
M J
MJ
Layet
C
C
Ferrier
P
P
Jordan
B R
BR
Lemonnier
F A
FA
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000939:Epitopes; D005796:Genes; D006680:HLA Antigens / Q000235:genetics* / Q000276:immunology; D015793:HLA-B7 Antigen; D006801:Humans; D007739:L Cells; D051379:Mice; D011110:Polymorphism, Genetic; D011863:Radioimmunoassay; D014162:Transfection
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006680:HLA Antigens; D015793:HLA-B7 Antigen