Escherichia coli tryptophan operon directs the in vivo synthesis of a leader peptide.
Here we report the identification of the Escherichia coli trp leader peptide synthesized in vivo. We identified the peptide in UV-irradiated maxicells by selective labeling with radioactive amino acids which are included in the predicted sequence of this peptide. Our results support the hypothesis that translation of the peptide-coding region of the leader RNA has a role in the mechanism of attenuation of biosynthetic operons in general and in the E. coli trp operon in particular.
L
L
Dekel-Gorodetsky
R
R
Schoulaker-Schwarz
H
H
Engelberg-Kulka
D000595:Amino Acid Sequence; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D009876:Operon; D014176:Protein Biosynthesis; D021382:Protein Sorting Signals / Q000096:biosynthesis* / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D014364:Tryptophan / Q000096:biosynthesis*
D021382:Protein Sorting Signals; D012329:RNA, Bacterial; D014364:Tryptophan