Formycin 3' end modified tRNATrp. Recognition by avian myeloblastosis virus reverse transcriptase and primer function.
Primer tRNATrp has been modified at the 3' end by adenosine analogues: 2'deoxyadenosine, 3'deoxyadenosine, 3' amino-3' deoxyadenosine and formycin. Aminoacylation of modified tRNATrp with cognate aminoacyl-tRNA synthetase and primer function for DNA synthesis catalyzed by AMV reverse transcriptase have been studied. The tRNATrp was able to accept tryptophan but did not initiate the DNA synthesis directed by 35S AMV RNA. Recognition of modified tRNATrp by AMV reverse transcriptase was not affected as followed by enzyme-tRNA complex formation. The functional consequences of these effects are discussed.
L
L
Sarih
M
M
Garret
F
F
von der Haar
B
B
Labouesse
S
S
Litvak
D000241:Adenosine / Q000031:analogs & derivatives; D000818:Animals; D000903:Antibiotics, Antineoplastic; D001354:Avian Leukosis Virus / Q000201:enzymology*; D009189:Avian Myeloblastosis Virus / Q000201:enzymology*; D002417:Cattle; D002847:Chromatography, Agarose; D002850:Chromatography, Gel; D004247:DNA / Q000096:biosynthesis*; D005573:Formycins; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*
D000903:Antibiotics, Antineoplastic; D005573:Formycins; D012346:RNA, Transfer, Amino Acyl; C034550:formycin; D004247:DNA; D012194:RNA-Directed DNA Polymerase; D000241:Adenosine