Effect of Nocardia rubra cell wall skeleton on murine interferon production in vitro.
Nocardia rubra cell wall skeleton (N-CWS) induced alpha- plus beta-interferons (IFN-alpha/beta) in resident peritoneal cells and bone marrow cells from normal mice. N-CWS also induced IFN-alpha/beta and IFN-gamma in the cultures of thioglycollate-elicited peritoneal cells. When induced with N-CWS, the mixed cultures of thioglycollate-elicited macrophages and spleen cells produced high titered IFN-gamma. Both macrophages and lymphocytes present in spleen cells (non-T, non-B, asialo-GM1-positive) were essential for the production. Immunofluorescence staining with anti-IFN-gamma antiserum showed the presence of IFN-gamma in lymphocytes but not in macrophages, suggesting that lymphocytes were producer cells and macrophages were accessory cells. Because IFN-gamma is an important lymphokine in immune response, the ability of N-CWS to stimulate IFN-gamma production may account for a significant portion of the antitumor activity of this agent.
Izumi
S
S
Ueda
H
H
Okuhara
M
M
Aoki
H
H
Yamamura
Y
Y
D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D017358:Cell Wall Skeleton; D002478:Cells, Cultured; D005260:Female; D007372:Interferons / Q000096:biosynthesis* / Q000276:immunology; D007694:Killer Cells, Natural / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D009088:Mucoproteins / Q000494:pharmacology*; D010529:Peritoneal Cavity / Q000166:cytology; D013154:Spleen / Q000378:metabolism; D013864:Thioglycolates / Q000494:pharmacology
D017358:Cell Wall Skeleton; D009088:Mucoproteins; D013864:Thioglycolates; C044553:cell wall skeleton, Nocardia; D007372:Interferons