The rat isolated mast cell as a model for the study of calcium antagonists.
EDTA-treated rat isolated mast cells were used to study the influence of Ca2+-antagonists on stimulus-secretion coupling. The concentration of Ca2+-effect curves for histamine release were parallelly shifted to the right when the cells were in the presence of Mg2+, La3+, verapamil or aminoglycoside antibiotics such as streptomycin, neomycin or gentamicin. The relative potency among the aminoglycoside was similar to that described in the literature for their action on the excitation-contraction coupling. The employed isolated mast cells preparation presented basic properties required for the study of coupled stimulus-secretion and represent an optimal model for the study of drugs which interfere with Ca2+ passage across cell membranes.
A M
AM
Wenzel
A P
AP
Corrado
W A
WA
Prado
D000617:Aminoglycosides / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D005260:Female; D006636:Histamine Release / Q000187:drug effects; D066298:In Vitro Techniques; D007811:Lanthanum / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000187:drug effects*; D008954:Models, Biological; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence; D014700:Verapamil / Q000494:pharmacology
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D002121:Calcium Channel Blockers; D000001:Calcimycin; D007811:Lanthanum; D004492:Edetic Acid; D014700:Verapamil; D008274:Magnesium