Endogenous type-C viral expression during lymphoma development in irradiated NFS mice.
The expression of the type-C retrovirus and the virus-related components in NFS mice were examined during preleukemic and leukemic phases after fractionated whole-body X irradiation. The NFS mice were highly susceptible to induction of thymoma by fractionated X irradiation. The leukemic tissues were negative for infectious type-C virus, as detected by both the XC-plaque test and mink S+ L- focus-inducing assays, but contained a substantially higher level of viral-specific RNA-dependent DNA polymerase activity and a major core protein p30 than the corresponding tissues from unirradiated age-control mice. In the preleukemic phase, the amount of p30-related antigen increased transiently in spleen. The leukemic cell lines established from radiation-induced lymphomas produced particulate entities with a buoyant density of about 1.15 g/ml. These virus-like particles lacked in vitro infectivity to mouse cells and mink lung cells and leukemogenicity in syngeneic mice. The p30-related antigens of these particles were immunologically similar to that of xenotropic virus derived from NZB mouse.
Okumoto
M
M
Nishikawa
R
R
Takamori
Y
Y
Iwai
Y
Y
Iwai
M
M
Tsubura
Y
Y
D000818:Animals; D005260:Female; D007953:Leukemia, Radiation-Induced / Q000209:etiology*; D008297:Male; D051379:Mice; D011289:Preleukemia / Q000209:etiology; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D012190:Retroviridae / Q000235:genetics*; D013945:Thymoma / Q000209:etiology; D013953:Thymus Neoplasms / Q000209:etiology; D014758:Viral Core Proteins / Q000032:analysis
D014758:Viral Core Proteins; D012194:RNA-Directed DNA Polymerase