Mn2+ dependent transformation of Bacillus subtilis.
The present study shows that transformation of Bacillus subtilis can proceed in the presence of Mn2+ instead of Mg2+ with equal efficiency. The crucial condition seems to be the Mn2+ concentration which should not exceed 0.025 mM. Binding, uptake and breakdown of donor DNA as well as its physicochemical fate in Mn2+ dependent transformation was investigated. No changes as compared to the standard procedure with Mg2+ were noticed. The possible source of errors in this kind of experiments is discussed.
Popowski
J
J
Bednarska
M
M
D001412:Bacillus subtilis / Q000235:genetics*; D004269:DNA, Bacterial / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology*; D014169:Transformation, Bacterial / Q000187:drug effects*
D004269:DNA, Bacterial; D008345:Manganese; D008274:Magnesium