Monoclonal antibodies to Nocardia asteroides and Nocardia brasiliensis antigens.
Nocardia asteroides and Nocardia brasiliensis whole-cell extracts were used as antigens to generate monoclonal antibodies (MAbs). Six stable hybrid cell lines secreting anti-Nocardia spp. MAbs were obtained. These were characterized by enzyme-linked immunosorbent assay, Western blot (immunoblot), and immunofluorescence assay. Although all the MAbs exhibited different degrees of cross-reactivity with N. asteroides and N. brasiliensis antigens as well as with culture-filtrate antigens from Mycobacteria spp., they have the potential for use as reagents in the purification of Nocardia antigens.
Department of Microbiology and Medical Zoology, School of Medicine, University of Puerto Rico, San Juan 00936.
T
T
Jiménez
A M
AM
Díaz
H
H
Zlotnik
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D015153:Blotting, Western; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D009161:Mycobacterium / Q000276:immunology; D009615:Nocardia / Q000276:immunology*; D009617:Nocardia Infections / Q000175:diagnosis; D009616:Nocardia asteroides / Q000276:immunology
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial