Calmodulin-binding proteins of Saccharomyces cerevisiae.
The subcellular distribution of calmodulin-binding proteins in the soluble, plasma membrane, and nuclear fractions of Saccharomyces cerevisiae was analyzed with a gel binding assay using 125I-labeled calmodulin. Over 20 binding proteins were detected. The calmodulin-binding protein profiles were markedly different among the fractions. Calmodulin-binding proteins were most abundant in the nuclear fraction, followed by the membrane fraction and the soluble fraction in decreasing order. The amounts of certain calmodulin-binding proteins increased after treatment with alpha-mating factor.
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Japan.
Y S
YS
Liu
Y
Y
Yamashita
E
E
Tsuchiya
T
T
Miyakawa
D002147:Calmodulin / Q000378:metabolism*; D002148:Calmodulin-Binding Proteins / Q000378:metabolism*; D002451:Cell Compartmentation; D005656:Fungal Proteins / Q000378:metabolism; D000072235:Mating Factor; D008970:Molecular Weight; D010455:Peptides / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D002147:Calmodulin; D002148:Calmodulin-Binding Proteins; D005656:Fungal Proteins; D010455:Peptides; D000072235:Mating Factor