[Effect of the culture technic on results following corneal preservation].
Organ culture of human donor material extends post mortem storage times up to 4 weeks. If dextrane is omitted from the medium, as in the authors' current technique, a stromal swelling occurs which can be reserved preoperatively. The authors also report on their experience with organ culture of donor material in a culture medium containing dextrane. Short-term incubation of donor tissue in culture medium at 32 degrees C containing dextrane may be possible as an alternative to the MK technique, in which the donor tissue is kept at 4 degrees C. All methods have specific advantages and disadvantages, not only in laboratory handling but also in the postoperative development of the graft.
M
M
Böhnke
J
J
Draeger
D
D
Bornemann
U
U
Steinhorst
D003315:Cornea / Q000473:pathology; D003316:Corneal Diseases / Q000601:surgery*; D016039:Corneal Transplantation; D003470:Culture Media; D003911:Dextrans; D004727:Endothelium / Q000473:pathology; D006801:Humans; D009926:Organ Preservation / Q000379:methods*; D011183:Postoperative Complications / Q000209:etiology
D003470:Culture Media; D003911:Dextrans
Einfluss der Kulturtechnik auf die Ergebnisse nach Hornhautkonservierung.