Detection of benzo[a]pyrene diol epoxide-DNA adducts in peripheral blood lymphocytes and antibodies to the adducts in serum from coke oven workers.
Coke oven workers are exposed to high levels of carcinogenic polycyclic aromatic hydrocarbons, including benzo[a]pyrene (B[a]P), and are at increased risk of lung cancer. Since B[a]P is enzymatically activated to 7 beta,8 alpha-dihydroxy(9 alpha, 10 alpha)epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (B[a]PDE) that forms adducts with DNA, the presence of these adducts was measured in DNA from peripheral blood lymphocytes by synchronous fluorescence spectrophotometry and enzyme radioimmunoassay. Approximately two-thirds of the workers had detectable levels of B[a]PDE-DNA adducts. Antibodies to the DNA adducts were also found in the serum of 27% of the workers. B[a]PDE-DNA adducts were not detectable in lymphocytes and antibodies to the adducts were not detected in sera from a control group of nonsmoking laboratory workers. DNA adducts and/or antibodies to the adducts indicate exposure to B[a]P and its metabolic activation to the carcinogenic metabolite that covalently binds to and damages DNA. Detection of adducts and antibodies to them may also be useful as internal dosimeters of the pathobiological effective doses of chemical carcinogens.
Harris
C C
CC
Vahakangas
K
K
Newman
M J
MJ
Trivers
G E
GE
Shamsuddin
A
A
Sinopoli
N
N
Mann
D L
DL
Wright
W E
WE
D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; D000906:Antibodies / Q000032:analysis*; D001580:Benzopyrenes / Q000378:metabolism*; D002274:Carcinogens, Environmental / Q000009:adverse effects / Q000032:analysis*; D003031:Coal; D003077:Coke; D004247:DNA / Q000378:metabolism*; D004784:Environmental Monitoring; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D008175:Lung Neoplasms / Q000139:chemically induced / Q000517:prevention & control; D008214:Lymphocytes / Q000032:analysis*; D009784:Occupational Diseases / Q000139:chemically induced / Q000517:prevention & control; D009790:Occupations; D011083:Polycyclic Compounds / Q000009:adverse effects / Q000032:analysis; D011863:Radioimmunoassay; D013050:Spectrometry, Fluorescence
D000906:Antibodies; D001580:Benzopyrenes; D002274:Carcinogens, Environmental; D003031:Coal; D003077:Coke; D000939:Epitopes; D011083:Polycyclic Compounds; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; D004247:DNA