A study of the NB/7OK and CA 125 monoclonal antibody radioimmunoassays for measuring serum antigen levels in ovarian cancer patients.
Serum levels of tumor antigens NB/7OK and CA 125 were determined in 101 samples by radioimmunoassays with the use of murine monoclonal antibodies. Among serum samples from ovarian cancer patients, elevated NB/7OK levels were found in 87% of samples that contained elevated CA 125 levels. With either assay, only 2% of patients with nonmalignant diseases had elevated antigen levels. No quantitative correlation was found, however, between levels of NB/7OK and CA 125. When antigen present in serum was purified by gel filtration on Sephacryl S-200, CA 125 eluted with an apparent molecular weight in excess of 100 kilodaltons whereas NB/7OK eluted with an apparent molecular weight of 70 kilodaltons. Thus the NB/7OK and CA 125 radioimmunoassays appear to measure two different populations of antigenic material.
S
S
Knauf
D J
DJ
Anderson
R C
RC
Knapp
R C
RC
Bast
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D015295:Antigens, Tumor-Associated, Carbohydrate; D000939:Epitopes / Q000032:analysis*; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000276:immunology*; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D015295:Antigens, Tumor-Associated, Carbohydrate; D000939:Epitopes