Human natural killer cell activity is reduced by treatment of anti-myelin-associated glycoprotein (MAG) monoclonal mouse IgM antibody and complement.
Human mononuclear cells could be stained by anti-myelin-associated glycoprotein (MAG) monoclonal mouse IgM antibody. The remaining human natural killer (NK) cell activity examined by using K-562 cells at 20:1 as effector:target ratio after treatment of anti-MAG monoclonal mouse or anti-Leu-7 (HNK-1) antibody and complement revealed 13.4% and 15.1%, respectively (untreated NK activity was 40.8%). However, human NK activity could be abrogated by anti-Leu-11 and complement. The remaining NK activity shown as lytic units after treatment with anti-MAG, anti-Leu-7 or anti-Leu-11 and complement was 6.1, 5.3 and below 1.0, respectively (untreated NK cells showed 15.4). When NK activity was examined in another target cell, MOLT-4, the remaining activity shown as lytic units was also decreased with anti-MAG antibody (4.3) or with anti-Leu-7 (3.0) (untreated NK activity was 8.3). Our findings suggest that NK cells may be influenced by anti-MAG antibody if it is found in the sera as anti-lymphocytotoxic antibody.
M
M
Tanaka
M
M
Nishizawa
T
T
Inuzuka
H
H
Baba
S
S
Sato
T
T
Miyatake
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000276:immunology; D003165:Complement System Proteins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009185:Myelin Proteins / Q000276:immunology*; D018992:Myelin-Associated Glycoprotein
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D000939:Epitopes; D007075:Immunoglobulin M; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D003165:Complement System Proteins