Blot-immunobinding test for the detection of anti-sperm antibodies.
A blot-immunobinding test was used to detect anti-sperm antibodies in human sera and to identify the corresponding auto- or iso-antigens on human sperm. A high proportion of sera at a 1:100 dilution from fertile persons, as well as infertile patients, contains antibodies reactive with sperm. This phenomenon might be physiological. At 1:2,000 dilution, a higher binding capacity was detected in the sera from infertile groups, but a few fertile persons were also positive. Antibodies to a single antigenic determinant with Mr of approximately 14,000 were found in a significantly higher proportion among males with unexplained infertility.
Lehmann
D
D
Temminck
B
B
Da Rugna
D
D
Leibundgut
B
B
Müller
H
H
D000906:Antibodies / Q000032:analysis*; D000911:Antibodies, Monoclonal; D001324:Autoantigens / Q000032:analysis; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D005260:Female; D006801:Humans; D007246:Infertility / Q000097:blood; D007519:Isoantigens / Q000032:analysis; D008297:Male; D008970:Molecular Weight; D010209:Paper; D013094:Spermatozoa / Q000276:immunology*
D000906:Antibodies; D000911:Antibodies, Monoclonal; D001324:Autoantigens; D000939:Epitopes; D007519:Isoantigens; D003101:Collodion