Antigen valence determines the binding of nominal antigen to cytolytic T cell clones.
We have shown that cytotoxic T cell clones specific for the nominal antigen FL will bind high molecular weight (600,000 to 2,000,000) polyacrylamide and Ficoll polymers conjugated with 200-600 FL groups per molecule. Low molecular weight polymers (40,000) with the same epitope density did not give stable binding. A high molecular weight polymer with a lower epitope density also failed to bind. Taken together, these results suggest that a substantial degree of multivalence is a necessary factor in the stable binding of nominal antigen to T cell clones.
Siliciano
R F
RF
Colello
R M
RM
Keegan
A D
AD
Dintzis
R Z
RZ
Dintzis
H M
HM
Shin
H S
HS
D000180:Acrylic Resins / Q000276:immunology; D000818:Animals; D000941:Antigens / Q000276:immunology*; D002999:Clone Cells / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D005362:Ficoll / Q000031:analogs & derivatives / Q000276:immunology; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives; D005452:Fluoresceins / Q000276:immunology; D051379:Mice; D008970:Molecular Weight; D009592:Nitrohydroxyiodophenylacetate / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D013861:Thiocyanates / Q000276:immunology; D000099166:Polyacrylamides
D000180:Acrylic Resins; D000941:Antigens; D000939:Epitopes; D005362:Ficoll; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D009592:Nitrohydroxyiodophenylacetate; D013861:Thiocyanates; D000099166:Polyacrylamides; C045540:fluorescein 5-isothiocyanate-Ficoll