Analysis of the specificity of five murine anti-blood group A monoclonal antibodies, including one that identifies type 3 and type 4 A determinants.
The specificity of five mouse monoclonal anti-A blood group antibodies (Ab), four of which were produced by immunization with cultured human cancer cells and one with a synthetic antigen, has been determined by examining their reactivity with purified A glycolipids, erythrocyte glycolipids, oligosaccharides, ovarian cyst glycoproteins, and salivary glycoproteins. Two of the antibodies (HT29-36 and CB) reacted with all A variant structures tested and have a broad anti-A reactivity. Ab CLH6 did not agglutinate A erythrocytes and reacted preferentially with the type 1A structure. Ab S12 agglutinated all A1 erythrocytes and reacted best with simple, monofucosyl type 2 A structures, such as Aa-2, Ab-2, and A tetrasaccharide. Ab M2 has a novel, but complex, spectrum of reactivity. It reacts with type 3 and type 4 A chains and not with type 1 and type 2 A chains. It appears to recognize both an external A structure (formula; see text) (I) (found) in type 3 and type 4 chains) and also an internal structure (II) found in type 3 chains. Ab M2 agglutinates all A and AB erythrocytes but does not react with salivary glycoproteins.
K
K
Furukawa
H
H
Clausen
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S
Hakomori
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J
Sakamoto
K
K
Look
A
A
Lundblad
M J
MJ
Mattes
K O
KO
Lloyd
D000017:ABO Blood-Group System / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D002460:Cell Line; D000939:Epitopes / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D006017:Glycolipids / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology; D006384:Hemagglutination; D006801:Humans; D051379:Mice; D009369:Neoplasms / Q000276:immunology; D009844:Oligosaccharides / Q000276:immunology
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D000939:Epitopes; D006017:Glycolipids; D006023:Glycoproteins; D009844:Oligosaccharides