AMLR and MLR stimulating activity of human T lymphocytes activated in vitro by soluble HLA-DR antigens.
We have examined the allogeneic mixed lymphocyte reaction (MLR) and autologous mixed lymphocyte reaction (AMLR) stimulating activities of T cells precultured in vitro with soluble allogeneic or autologous HLA-DR antigens. These cells (Ts) are known to suppress the human MLR: this suppression is specific in that it occurs only when stimulator cells have the same HLA-DR antigen as that used to induce differentiation of suppressor cells. Ts cells express new membrane specificities; they can be separated by immunoabsorption into two populations: Ts enriched (Tx+; with suppressive activity) and Ts depleted (Ts-; with helper function). In the present study, we have demonstrated that both Ts cell subsets activated by soluble HLA-DR alloantigens are able to stimulate both MLR and AMLR. Ts cells activated by soluble autologous HLA-DR antigens are able to stimulate MLR, but not AMLR.
M
M
Vanoli
R
R
Scorza Smeraldi
G
G
Fabio
P
P
Bonara
M G
MG
Sabbadini
C
C
Zanussi
D000328:Adult; D002478:Cells, Cultured; D000939:Epitopes / Q000276:immunology; D006684:HLA-DR Antigens; D015805:HLA-DR5 Antigen; D015809:HLA-DR7 Antigen; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D012995:Solubility; D013601:T-Lymphocytes / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D000939:Epitopes; D006684:HLA-DR Antigens; D015805:HLA-DR5 Antigen; D015809:HLA-DR7 Antigen; D000949:Histocompatibility Antigens Class II