Molecular cloning of cDNA for the human tumor-associated antigen CO-029 and identification of related transmembrane antigens.
The human tumor-associated antigen CO-029 is a monoclonal antibody-defined cell surface glycoprotein of 27-34 kDa. By using the high-efficiency COS cell expression system, a full-length cDNA clone for CO-029 was isolated. When transiently expressed in COS cells, the cDNA clone directed the synthesis of an antigen reactive to monoclonal antibody CO-029 in mixed hemadsorption and immunoblot assays. Sequence analysis revealed that CO-029 belongs to a family of cell surface antigens that includes the melanoma-associated antigen ME491, the leukocyte cell surface antigen CD37, and the Sm23 antigen of the parasitic helminth Schistosoma mansoni. CO-029 and ME491 antigen expression and the effect of their corresponding monoclonal antibodies on cell growth were compared in human tumor cell lines of various histologic origins.
Szala
S
S
Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
Kasai
Y
Y
Steplewski
Z
Z
Rodeck
U
U
Koprowski
H
H
Linnenbach
A J
AJ
D000595:Amino Acid Sequence; D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000235:genetics*; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics*; D008961:Models, Structural; D008969:Molecular Sequence Data; D011487:Protein Conformation; D012689:Sequence Homology, Nucleic Acid; D059470:Tetraspanins; D014162:Transfection
D000951:Antigens, Neoplasm; D004273:DNA, Neoplasm; D008562:Membrane Glycoproteins; C065609:TSPAN8 protein, human; D059470:Tetraspanins