Characterization of interferon induced in murine macrophage cultures by 10-carboxymethyl-9-acridanone.
Pure murine macrophages were induced by 10-carboxymethyl-9-acridanone to produce interferon. The supernatants were partially purified by a three-column procedure including a DEAE-Biogel A, a CM-Biogel A, and a CH-Sepharose 4B column. The specific activity achieved was about 10(5) IU/mg. Two different activities were detected after the third step and designated activity 1 and activity 2. The determination of the molecular weight was in the range of 24,000-27,000 dalton for activity 1 and in the range of 32,000-34,000 dalton for activity 2. Both were neutralized by antibodies against mouse interferon-beta, indicating that two different moieties were produced both representing interferon-beta. When macrophages were induced in the presence of tunicamycin, only one activity of a molecular weight of about 19,000 dalton was found which again was neutralized by anti-interferon-beta.
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Brehm
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Storch
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Kirchner
D000166:Acridines / Q000494:pharmacology*; D000818:Animals; D000906:Antibodies / Q000276:immunology; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D007370:Interferon Type I / Q000276:immunology; D007372:Interferons / Q000096:biosynthesis / Q000302:isolation & purification*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D009500:Neutralization Tests; D014415:Tunicamycin / Q000494:pharmacology
D000166:Acridines; D000906:Antibodies; D007370:Interferon Type I; D014415:Tunicamycin; D007372:Interferons; C026430:10-carboxymethyl-9-acridanone