Continuous production of a cytopathic human T-lymphotropic virus in a permissive neoplastic T-cell line.
We developed cloned populations from the commonly available, well-characterized cell line HUT-78. These cloned cells grow permanently after infection with isolates of human T-lymphotropic virus type III, also called lymphadenopathy virus (HTLV-III/LAV), from patients with acquired immune deficiency syndrome and related syndromes. In contrast, activated human T cells are lysed after HTLV-III/LAV infection. The infected cloned cells have been in culture continuously for 6 months and have produced high levels of extracellular reverse transcriptase (400,000 cpm/ml). This level is comparable to that of similarly infected normal human T cells. Three weeks after infection with HTLV-III/LAV, more than 90% of the cloned HUT-78 cells lysed; the remaining cells continued to grow. Approximately 80% of these cells expressed HTLV-III/LAV antigens by immunofluorescence. The extracellular virus of the chronically infected cell line was shown to be similar to other HTLV-III/LAV isolates by competition radioimmunoassay, by reactivity with human serum, and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This HTLV-III/LAV-infected immortalized cell line enables the continuous production of large amounts of virus.
Getchell
J P
JP
Heath
J L
JL
Hicks
D R
DR
Sporborg
C
C
McGrath
C R
CR
Kalyanaraman
V S
VS
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology; D000956:Antigens, Viral / Q000276:immunology; D002460:Cell Line; D002999:Clone Cells; D017977:Deltaretrovirus / Q000254:growth & development* / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D015488:HIV Antigens; D006801:Humans; D008297:Male; D012194:RNA-Directed DNA Polymerase / Q000096:biosynthesis; D011863:Radioimmunoassay; D013601:T-Lymphocytes / Q000382:microbiology*; D014776:Virus Cultivation
D000956:Antigens, Viral; D015488:HIV Antigens; D012194:RNA-Directed DNA Polymerase