Isolation of cellulolytic mutants of thermotolerant fungus Chaetomium cellulolyticum ATCC 32319.
Spores of Chaetomium cellulolyticum were treated with 200 micrograms/ml of N-methyl-N'-nitro-N-nitrosoguanidine and seven mutants producing clear zones around their colonies on modified Vogels medium were isolated. Mutant NG7 showed altered morphological characteristics and produced more cellulases (CMCase--15 units, FPA--6.5 units, CDA--0.80 units and cellobiase--4.7 units/ml) than its parental strain (CMCase--10 units, FPA--4.5 units, CDA--0.36 units and cellobiase--2.7 units/ml). Cellulase preparation was used to saccharify rice straw, wheat straw, bagasse and sawdust, pretreated with 1% sodium hydroxide.
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Kotia
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Dhillon
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Singh
D001203:Ascomycota / Q000201:enzymology*; D002480:Cellulase / Q000096:biosynthesis* / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism; D002597:Chaetomium / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D006026:Glycoside Hydrolases / Q000378:metabolism; D003368:Gossypium; D009154:Mutation; D013172:Spores, Fungal; D001617:beta-Glucosidase / Q000378:metabolism
D002482:Cellulose; D006026:Glycoside Hydrolases; D001617:beta-Glucosidase; D002480:Cellulase; C019826:carboxymethylcellulase