The ribonucleotide reductase induced by herpes simplex virus type 1 involves minimally a complex of two polypeptides (136K and 38K).
Herpes simplex virus type 1 (HSV-1) encodes a polypeptide of apparent mol. wt. 136 000 (Vmw136) known to be a component of the virus-specified ribonucleotide reductase. Monoclonal antibodies that precipitate this polypeptide also precipitate a polypeptide of mol. wt. 38 000 (Vmw38) from extracts of HSV-1-infected cells. The basis for this co-precipitation has been investigated using a monoclonal antibody directed against Vmw136 and an oligopeptide-induced antiserum directed against the carboxy terminus of Vmw38. We have also made use of a temperature-sensitive (ts) mutant of HSV-1 which maps within the sequences encoding Vmw136 and which induces a thermolabile ribonucleotide reductase. Our experiments show (i) Vmw136 and Vmw38 form a complex in infected cells and (ii) the mutation in the ts mutant results in the two polypeptides being unable to form the complex at the non-permissive temperature. We speculate that association of the two polypeptides is necessary for ribonucleotide reductase activity. No evidence was found for involvement of host proteins in the proposed virus-induced ribonucleotide reductase complex. The terms RR1 and RR2 are suggested for the large and small subunits of the HSV-induced enzyme.
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M C
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Marsden
H S
HS
Dutia
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BM
D000911:Antibodies, Monoclonal; D004790:Enzyme Induction; D000939:Epitopes; D008970:Molecular Weight; D009154:Mutation; D010455:Peptides; D011233:Precipitin Tests; D012264:Ribonucleotide Reductases / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D018139:Simplexvirus / Q000201:enzymology* / Q000235:genetics; D013696:Temperature
D000911:Antibodies, Monoclonal; D000939:Epitopes; D010455:Peptides; D012264:Ribonucleotide Reductases