[Preparation and characteristics of monoclonal antibodies against GTP-binding protein from the bovine retina].
Five monoclonal antibodies against the native GTP-binding protein (transducin) from bovine retina have been prepared. By immunoblotting and immunoenzymatic analysis of the isolated alpha- and gamma-subunits of transducin and the beta gamma-subunit complex it was determined that two monoclonal antibodies A3G7 and A3C10 recognize linear antigenic determinants on the alpha-subunit, two other, A3E4 and 3B3, bound specifically to the gamma-subunit, and monoclonal antibodies 1C3 interact only with native transducin. Both antibodies against the alpha-subunit inhibited transducin GTPase activity, whereas antibodies A3E4, 3B3 and 1C3 did not affect it.
Shamborant
O G
OG
Kolosov
M I
MI
Vasilov
R G
RG
Lipkin
V M
VM
Ovchinnikov
Iu A
IuA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D020558:GTP Phosphohydrolases / Q000037:antagonists & inhibitors; D019204:GTP-Binding Proteins / Q000032:analysis / Q000276:immunology*; D007124:Immunoenzyme Techniques; D007525:Isoelectric Focusing; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D012160:Retina / Q000032:analysis*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins
Poluchenie i kharakteristika monoklonal'nykh antitel k GTP-sviazyvaiushchemu belku iz setchatki glaz byka.