[H-phosphonate method in the synthesis of structural gene of human interleukin-4].
A modified H-phosphonate method was used to synthesize 32 oligodeoxyribonucleotides ranging in length from 23 to 28, which were enzymatically joined together to give the human interleukin 4 gene. The high degree of the oligonucleotide purity, achieved through the application of anion-exchange and reverse phase HPLC, ensures the high percentage of the desired sequence (about 75%) in the cloned DNA.
Kulagina
M A
MA
Skaptsova
N V
NV
Batchikova
N V
NV
Kurkin
A N
AN
Azhaev
A V
AV
D000595:Amino Acid Sequence; D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D005796:Genes; D005813:Genes, Synthetic; D006801:Humans; D015847:Interleukin-4 / Q000235:genetics*; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D063065:Organophosphonates
D015345:Oligonucleotide Probes; D063065:Organophosphonates; D015847:Interleukin-4
H-fosfonatnyĭ metod v sinteze strukturnogo gena interleĭkina-4 cheloveka.