Quantifying the MB isoenzyme of creatine kinase with the Abbott "IMx" immunoassay analyzer.
In this two-step automated assay of the MB isoenzyme of creatine kinase (CK-MB), developed for the Abbott "IMx" immunoassay analyzer, monoclonal anti-CK-MB antibody immobilized onto latex microparticles and polyclonal anti-CK-MM antibody coupled to alkaline phosphatase are used. Within-run CVs ranged from 3.9% to 9.0%, between-run CVs from 0.0% to 5.6%, and the sensitivity was 0.2 microgram/L. Twenty-four results can be obtained in about 37 min. Analytical recovery of CK-MB added to human serum or plasma ranged from 89% to 109%. Icteric, lipemic, or hemolyzed samples did not interfere with CK-MB recovery. Cross-reactivity with CK-MM and CK-BB was 0.012% and 0.001%, respectively. The normal reference interval was 0-5 micrograms/L. IMx CK-MB results correlated well with CK-MB enzyme activity as determined by electrophoresis (n = 203; r = 0.97; slope = 0.90; y-intercept = -4.29) and with commercial immunoassays. We think that this assay will be useful for confirmation of acute myocardial infarction, both in critical-care units and in the clinical laboratory.
Department of Physiological Diagnostics, Abbott Diagnostics Division, Abbott Laboratories, IL 60064.
D R
DR
Brandt
R C
RC
Gates
K K
KK
Eng
C M
CM
Forsythe
G K
GK
Korom
A S
AS
Nitro
P A
PA
Koffler
E A
EA
Ogunro
D000911:Antibodies, Monoclonal / Q000032:analysis; D001322:Autoanalysis / Q000295:instrumentation; D003402:Creatine Kinase / Q000032:analysis* / Q000276:immunology / Q000592:standards; D003429:Cross Reactions; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D007124:Immunoenzyme Techniques; D007527:Isoenzymes; D008297:Male; D009203:Myocardial Infarction / Q000175:diagnosis / Q000201:enzymology*; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D012016:Reference Values
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D011933:Reagent Kits, Diagnostic; D003402:Creatine Kinase