Immunochemical detection of photoaffinity-labelled capsaicin-binding proteins from sensory neurons.
Capsaicin is a plant neurotoxin which depolarises a subset of mammalian sensory neurons. A photoaffinity probe (4-azidophenylpropionamide) with capsaicin-like agonist activity (EC50 5 microM) has been used to covalently label rat and chick sensory neurons in culture, as well as membrane preparations from both neurons and other tissues. Dorsal root ganglion cell specific capsaicin-binding proteins, including a major band of apparent molecular mass 58,000, have been identified by means of Western blotting, using a specific anti-capsaicin antiserum characterised by radioimmunoassay with a large range of capsaicin congeners. Using the same radioimmunoassay, no endogenous capsaicin-like immunoreactive material in normal or inflamed tissue has, however, been detected.
Sandoz Institute for Medical Research, London, UK.
J N
JN
Wood
C
C
Walpole
I F
IF
James
A
A
Dray
P R
PR
Coote
D000345:Affinity Labels / Q000378:metabolism*; D000818:Animals; D001386:Azides / Q000378:metabolism; D015153:Blotting, Western; D002211:Capsaicin / Q000378:metabolism*; D002352:Carrier Proteins / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D002531:Cerebellum / Q000378:metabolism; D002645:Chickens; D005727:Ganglia, Spinal / Q000378:metabolism; D007106:Immune Sera; D008099:Liver / Q000378:metabolism; D008970:Molecular Weight; D009475:Neurons, Afferent / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats
D000345:Affinity Labels; D001386:Azides; D002352:Carrier Proteins; D007106:Immune Sera; C065795:4-azidophenylpropionamide; D002211:Capsaicin