Biochemical characterisation of A blood group activity on human platelets.
A butanol extraction procedure has been used to isolate the blood group A antigen determinants from platelet membranes and to determine their biochemical structure-glycolipid and/or glycoprotein. The blood group A serological activity was found to reside entirely in the butanol (organic) fraction. When this fraction was analyzed by thin-layer chromatography, the A-inhibitory activity was found to comigrate with extracts of erythrocyte type Aa, Ab and Ac variant structures. The results of this study indicate that the ABO blood group A determinants on human platelets are glycolipid, similar in structure to glycolipid A determinants on erythrocytes.
R A
RA
Dunstan
C M
CM
Mansbach
D000017:ABO Blood-Group System / Q000276:immunology*; D001792:Blood Platelets / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology; D000939:Epitopes / Q000302:isolation & purification; D006017:Glycolipids / Q000097:blood / Q000276:immunology; D006801:Humans; D008563:Membrane Lipids / Q000097:blood / Q000276:immunology
D000017:ABO Blood-Group System; D000939:Epitopes; D006017:Glycolipids; D008563:Membrane Lipids