The ultrastructural labelling of keratin proteins in normal human colonic mucosa by a low temperature embedding, protein A-gold technique.
A low temperature embedding technique using protein A-gold as a marker was used to define the distribution of keratin proteins at the ultrastructural level in normal colonic epithelial cells. In both goblet and absorptive cells, keratin proteins were found at the base and lateral borders of the cell and inserting into desmosomes. There was a network of keratin filaments which surrounded cell organelles. In goblet cells, bundles of keratin filaments surrounded mucin droplets. In absorptive cells, a fine network of keratin filaments interdigitated with the terminal web. These studies with an immunochemical technique defined the keratin cytoskeleton at the ultrastructural level.
M J
MJ
Warhol
J
J
Roth
D003106:Colon / Q000648:ultrastructure*; D006046:Gold; D006801:Humans; D007120:Immunochemistry; D007413:Intestinal Mucosa / Q000648:ultrastructure*; D007633:Keratins; D008854:Microscopy, Electron; D013205:Staphylococcal Protein A
D013205:Staphylococcal Protein A; D007633:Keratins; D006046:Gold