Detection of cell surface sodium channels by monoclonal antibodies--could the channels become exposed to the external surface and 'down regulated' by binding to antibodies?
Different domains of the sodium channel were characterized in rat sciatic nerve according to the binding of antibodies to their antigenic determinants. An extracellular domain, accessible to antibodies, modulates channel conductance. Another domain, which is not involved in the physiological activity, becomes accessible to externally applied antibodies only after prolonged exposure. This study also revealed mobile antigenic determinants whose internalization can be detected by immunofluorescence.
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D000818:Animals; D000911:Antibodies, Monoclonal; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism; D000939:Epitopes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D007473:Ion Channels / Q000276:immunology / Q000378:metabolism*; D010525:Peripheral Nerves / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D012964:Sodium / Q000378:metabolism*; D015222:Sodium Channels
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D000939:Epitopes; D007473:Ion Channels; D015222:Sodium Channels; C023790:tetrodotoxin-binding protein; D012964:Sodium