Endothelin: a novel peptide in the posterior pituitary system.
Endothelin (ET), originally characterized as a 21-residue vasoconstrictor peptide from endothelial cells, is present in the porcine spinal cord and may act as a neuropeptide. Endothelin-like immunoreactivity has now been demonstrated by immunohistochemistry in the paraventricular and supraoptic nuclear neurons and their terminals in the posterior pituitary of the pig and the rat. The presence of ET in the porcine hypothalamus was confirmed by reversed-phase high-pressure liquid chromatography and radioimmunoassay. Moreover, in situ hybridization demonstrated ET messenger RNA in porcine paraventricular nuclear neurons. Endothelin-like immunoreactive products in the posterior pituitary of the rat were depleted by water deprivation, suggesting a release of ET under physiological conditions. These findings indicate that ET is synthesized in the posterior pituitary system and may be involved in neurosecretory functions.
Department of Neurology, University of Tsukuba, Japan.
T
T
Yoshizawa
O
O
Shinmi
A
A
Giaid
M
M
Yanagisawa
S J
SJ
Gibson
S
S
Kimura
Y
Y
Uchiyama
J M
JM
Polak
T
T
Masaki
I
I
Kanazawa
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D016232:Endothelins; D004730:Endothelium, Vascular; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000032:analysis; D009693:Nucleic Acid Hybridization; D010286:Paraventricular Hypothalamic Nucleus / Q000032:analysis; D010455:Peptides / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D010902:Pituitary Gland / Q000032:analysis* / Q000378:metabolism; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013495:Supraoptic Nucleus / Q000032:analysis; D013552:Swine; D014018:Tissue Distribution; D014868:Water Deprivation
D016232:Endothelins; D010455:Peptides; D015347:RNA Probes; D012333:RNA, Messenger