Somatostatin, substance P and [Lys]-vasopressin interfere with the binding of [3H][D-Ala2, Met5]enkephalinamide to rat brain membranes.
Somatostatin and substance P (10(-6)-3 X 10(-5) M) inhibited dose-dependently the specific binding of [3H] [D-Ala2, Met5]enkephalinamide [( 3H]-DALAMID) to rat brain membranes, by decreasing the number of binding sites. [Lys]-Vasopressin also inhibited the binding, by affecting both the number of binding sites and the affinity of receptors to [3H]DALAMID. The results indicate that somatostatin and substance P may interfere with the endorphin system by an apparently competitive manner.
Maruyama
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M
D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D001921:Brain / Q000378:metabolism*; D004744:Enkephalin, Methionine / Q000031:analogs & derivatives* / Q000378:metabolism; D066298:In Vitro Techniques; D008236:Lypressin / Q000494:pharmacology*; D008297:Male; D008566:Membranes / Q000378:metabolism; D051381:Rats; D013004:Somatostatin / Q000494:pharmacology*; D013373:Substance P / Q000494:pharmacology*
D013373:Substance P; D008236:Lypressin; D013004:Somatostatin; D004744:Enkephalin, Methionine; C022118:enkephalinamide-Met, Ala(2)-