[Purification, quaternary structure and immunological properties of phosphorylase kinase from chicken skeletal muscle].
Phosphorylase kinase was isolated from red and white chicken skeletal muscle in a nearly homogeneous state as judged by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate. The molecular weight of the native enzyme as determined by gel filtration on Sepharose 4B is close to that of rabbit skeletal muscle phosphorylase kinase (i. e., approximately 1300 000). The molecular weights of the subunits determined by SDS gel electrophoresis are: alpha', 140 000 beta, 129 000; gamma', 44 000; delta, 17 000 (cf. the Mr values of the alpha- and gamma-subunits of the rabbit muscle isoenzyme are 146 000 and 42 000). The four subunits, alpha', beta, gamma' and delta, were found to exist in equimolar amounts as shown by a densitometric analysis of acrylamide gels; hence, the subunit formula of the chicken skeletal muscle isoenzyme is (alpha' beta gamma' delta)4. Rabbit antisera against a mixture of alpha'- and beta-subunits of chicken phosphorylase kinase yield a single precipitin line with this enzyme, do not show cross reactions of identity with the rabbit muscle enzyme but strongly inhibit the activity of the chicken enzyme and partially inhibit the activity of the rabbit muscle isoenzyme.
Andreeva
I E
IE
Silonova
G V
GV
Livanova
N B
NB
Eronina
T B
TB
Morozov
V E
VE
D000818:Animals; D002645:Chickens; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D005779:Immunodiffusion; D046911:Macromolecular Substances; D008970:Molecular Weight; D009132:Muscles / Q000201:enzymology*; D010764:Phosphorylase Kinase / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D011817:Rabbits; D013045:Species Specificity
D000939:Epitopes; D046911:Macromolecular Substances; D010764:Phosphorylase Kinase
Ochistka, chetvertichnaia struktura i nekotorye immunologicheskie svoĭstva kinazy fosforilazy iz skeletnykh myshts kuritsy.