Multiplication in liquid medium of Treponema sp. isolated from intestinal contents of swine.
Treponema hyodysenteriae and Treponema innocens were multiplied by a simple culture method in liquid medium. TSB medium was prepared by the PRAS method in plasma bottles containing glass beads. Spirochaetes were injected through the rubber stopper and the bottles were incubated while revolving round their axes. The most abundant growth of spirochaetes in rotary culture was observed after 72 h incubation at 40 degrees C. whereas the highest number of viable cells in stationary culture was observed after 120 h. However, in the latter case the number of cells was lower than introduced at inoculation. Growth of the bacteria was stimulated by equine serum and 5% addition of rumen fluid. Optimal growth temperature was 40 degrees C.
Binek
M
M
Szynkiewicz
Z
Z
D000818:Animals; D001431:Bacteriological Techniques; D003470:Culture Media; D005766:Gastrointestinal Contents; D007422:Intestines / Q000382:microbiology; D013045:Species Specificity; D013552:Swine / Q000382:microbiology; D013696:Temperature; D013997:Time Factors; D014208:Treponema / Q000254:growth & development* / Q000302:isolation & purification
D003470:Culture Media