Interaction of endothelial cell growth factor with heparin: characterization by receptor and antibody recognition.
Endothelial cell growth factor (ECGF) binds specifically in vitro to membrane receptors present on the surface of several cell types, including murine and human endothelial cells and fibroblasts. Monoclonal antibodies prepared against ECGF that inhibit the mitogenic activity of the growth factor prevent receptor occupancy by the ligand. Heparin interacts structurally with ECGF [Maciag, T., Mehlman, T., Friesel, R. & Schreiber, A. B. (1984) Science 225, 932-935], potentiates the mitogenic activity of the polypeptide, restores the biological activity to inactivate ECGF, enhances the affinity of the ligand to cell surface receptors, and modifies antibody recognition of ECGF. These data suggest that the association between heparin and ECGF induces a conformational change in the polypeptide that increases or stabilizes the biological activity of the mitogen.
A B
AB
Schreiber
J
J
Kenney
W J
WJ
Kowalski
R
R
Friesel
T
T
Mehlman
T
T
Maciag
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016228:Endothelial Growth Factors; D004727:Endothelium / Q000378:metabolism*; D000939:Epitopes; D005347:Fibroblasts / Q000378:metabolism; D006133:Growth Substances / Q000276:immunology / Q000378:metabolism*; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D008938:Mitosis; D011485:Protein Binding / Q000187:drug effects; D011487:Protein Conformation / Q000187:drug effects; D011975:Receptors, Mitogen / Q000378:metabolism*; D040262:Receptors, Vascular Endothelial Growth Factor
D016228:Endothelial Growth Factors; D000939:Epitopes; D006133:Growth Substances; D011975:Receptors, Mitogen; D006493:Heparin; D040262:Receptors, Vascular Endothelial Growth Factor