In vitro production of a human HLA alloantibody of restricted specificity (DQw2) via Epstein-Barr virus transformation.
This paper describes the in vitro production of the human alloantibody 454A.C5 with HLA-DQw2 specificity produced by a stable EBV-transformed cell line. Immune B cells for transformation were generated by planned immunization of a blood donor volunteer with repeated HLA-immunizations over the course of 9 yr. Reactivity of 454A.C5 was in exact concordance with the presence of the DQw2 antigen as defined by microcytotoxicity assay on a panel of 21 B lymphoblastoid cell lines with well-characterized HLA antigens. Family studies showed the segregation according to the HLA-DQw2 haplotype; thus we demonstrate by serological and formal genetics criteria, that the alloantibody is specifically directed against the DQw2. 454A.C5 antibody may be employed as a monospecific HLA typing reagent.
Pistillo
M P
MP
Hämmerling
U
U
Dupont
B
B
Ferrara
G B
GB
D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D002472:Cell Transformation, Viral; D000939:Epitopes / Q000032:analysis*; D006680:HLA Antigens / Q000276:immunology*; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D007117:Immunization, Secondary; D007518:Isoantibodies / Q000276:immunology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology
D000939:Epitopes; D006680:HLA Antigens; D007518:Isoantibodies