1.5 kb mRNA abundantly expressed in rat tumors encodes a 37 kilodalton protein in vitro.
A cDNA clone, pAH34, corresponding to a 1.5 kb mRNA present abundantly in various rat tumors was examined for its protein coding capacity. Hybridization-selected RNAs from both poly(A)+ RNAs of a rat ascites hepatoma cell line, AH60C and of normal liver produced a polypeptide of 37 kilo daltons in vitro, but at much higher levels in the AH60C than in the normal rat liver. Two dimensional electrophoresis of the translation product revealed that the pI of this protein was 7.1. Nucleotide sequence analysis of pAH34 showed that the insert of the clone consisted of 462bp and contained the 3' portion of mRNA, including poly(A) stretch with AATAAA signal sequence centered 16 nucleotides upstream, a short untranslated region and an open reading frame corresponding to possibly 67 amino acids of the C-terminal portion.
Maehara
Y
Y
Fujiyoshi
T
T
Takahashi
K
K
Yamamoto
M
M
Endo
H
H
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000378:metabolism; D004274:DNA, Recombinant / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000235:genetics*; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000378:metabolism; D014176:Protein Biosynthesis; D021382:Protein Sorting Signals; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D051381:Rats
D004274:DNA, Recombinant; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA