Chlordecone toxicity: effect of withdrawal of treatment on ATPase inhibition.
Adult male Sprague-Dawley rats were treated P.O with 10 mg/kg/day chlordecone for 10 days. Five rats from control group receiving corn oil and five rats from chlordecone group were sacrificed for tissue preparations. The remaining rats in chlordecone group were withdrawn from treatment and left in cages for 45 days. At 15, 30 and 45 days after withdrawal, 5 rats from each group with equal number of controls were sacrificed. Brain, liver and kidney were removed and subcellular fractions were prepared. Na+-K+, oligomycin-sensitive and oligomycin-insensitive Mg2+ ATPases were determined. Rats treated with chlordecone for 10 days showed a significant reduction of Na+-K+ ATPase activity in brain and kidney. The decreased enzyme activity in kidney but not in brain returned to normal within 15 days of treatment withdrawal. In brain the enzyme activity stayed at reduced level throughout the experimental period. Oligomycin-sensitive Mg2+ ATPase activity in all the tissues was decreased significantly in chlordecone treated rats. The enzyme activity returned to normal levels in all tissues gradually by 30 days of treatment withdrawal. Oligomycin-insensitive Mg2+ ATPase activity was not decreased in any tissue by chlordecone treatment. These results suggest that chlordecone effects on ATPase system are reversible except for Na+-K+ ATPase in brain.
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D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors*; D000818:Animals; D001921:Brain / Q000201:enzymology; D007631:Chlordecone / Q000633:toxicity*; D007306:Insecticides / Q000633:toxicity*; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D009840:Oligomycins / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors; D013347:Subcellular Fractions / Q000201:enzymology; D013997:Time Factors
D007306:Insecticides; D009840:Oligomycins; D000251:Adenosine Triphosphatases; D000254:Sodium-Potassium-Exchanging ATPase; D007631:Chlordecone