[Electrophoresis of urinary proteins on SDS-polyacrylamide gel, without preliminary concentration].
We described a rapid and sensitive method for urinary proteins disc-electrophoresis on unconcentrated urine, by using polyacrylamide-SDS gel in discontinuous gradient. Correlation with a conventional polyacrylamide agarose slab electrophoresis on concentrated urine is good, our technique being more sensitive when proteinuria is low. It had proved to be useful for routine detection of renal failures in clinical laboratories.
Coquelin
H
H
Cloppet
H
H
Boucaud-Maitre
Y
Y
Francina
A
A
Hutinel
P
P
D000180:Acrylic Resins; D000419:Albuminuria / Q000652:urine; D000510:Alpha-Globulins / Q000652:urine; D004589:Electrophoresis, Disc / Q000379:methods; D006801:Humans; D008970:Molecular Weight; D009194:Myeloma Proteins / Q000652:urine; D011507:Proteinuria / Q000652:urine*; D012967:Sodium Dodecyl Sulfate; D014168:Transferrin / Q000652:urine; D014556:Urine / Q000032:analysis*; D001613:beta 2-Microglobulin / Q000652:urine; D000099166:Polyacrylamides
D000180:Acrylic Resins; D000510:Alpha-Globulins; D009194:Myeloma Proteins; D012967:Sodium Dodecyl Sulfate; D014168:Transferrin; D001613:beta 2-Microglobulin; D000099166:Polyacrylamides
Electrophorèse des protéines urinaires en gel de polyacrylamide-SDS sans concentration préalable.