Analysis of serum placental alkaline phosphatase activity in testicular cancer and cigarette smokers.
Serum PLAP/PLAP-like enzymes are elevated in testicular cancer patients and also in heavy smokers. Such elevations were measurable with anti-PLAP polyclonal and monoclonal antibodies, and an antigenic heterogeneity was demonstrated in the circulating enzymes. Thus polyclonal antibody revealed more of tumor PLAP-like antigen while a monoclonal antibody H7 detected PLAP of smokers better. PLAP of smokers and PLAP-like enzyme of cancer (seminoma) patients showed similar characteristics with respect to glycosylation pattern, charge and hydrophobicity. By these properties they were differentiated from PLAP of placenta.
Koshida
K
K
Department of Virology, National Bacteriological Laboratory, Stockholm, Sweden.
Stigbrand
T
T
Munck-Wikland
E
E
Hisazumi
H
H
Wahren
B
B
D000469:Alkaline Phosphatase / Q000097:blood*; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000097:blood*; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D004407:Dysgerminoma / Q000201:enzymology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D058851:GPI-Linked Proteins; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000097:blood*; D008297:Male; D012907:Smoking / Q000097:blood*; D013736:Testicular Neoplasms / Q000201:enzymology*
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D058851:GPI-Linked Proteins; D007527:Isoenzymes; D000469:Alkaline Phosphatase; C019839:alkaline phosphatase, placental