Solid-phase adsorption of antigens for efficient production of antibodies reactive with native and fixed tissue antigens.
A study has been made of the efficacy of different immunization protocols using low antigen levels for the generation of monoclonal antibodies capable of detecting antigens (ADCP) in processed tissues. Protocols using unprocessed native antigen immobilized on nitrocellulose were more efficient than soluble antigen in generating serum antibodies reactive with both native antigen and processed tissues. The derived monoclonal antibodies reacted with native but not processed antigen. The use of antigen immobilized on polyvinylidene (PVDF) and subsequently processed as for histochemistry was successful in generating monoclonal antibodies reactive with processed antigen.
Dinjens
W N
WN
Department of Pathology, University of Limburg, Maastricht, The Netherlands.
Van der Linden
E P
EP
Signet
C M
CM
Wijnen
J T
JT
Meera Khan
P
P
Ten Kate
J
J
Bosman
F T
FT
D000243:Adenosine Deaminase / Q000276:immunology*; D000327:Adsorption; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D018819:Dipeptidyl Peptidase 4; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D007114:Immunization; D007150:Immunohistochemistry; D007158:Immunologic Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D009700:Nucleoside Deaminases / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; C042807:DPP4 protein, human; D018819:Dipeptidyl Peptidase 4; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase