Studies of monoclonal antibodies specific for major histocompatibility complex products of the rat. III. Production and characterization of syngeneic and allogeneic monoclonal anti-idiotypic antibodies to monoclonal alloantibodies.
Sixteen monoclonal anti-idiotypic antibodies were prepared against RT1 antigen-specific monoclonal antibodies by means of an inhibition assay. Three (110-3, 112-89, and 133-4) were produced against antibody 3-8C2 (19), three (178-60, 181-14, and 184-38) against antibody 11/23/5, and three (144-3, 144-33, and 146-31) against antibody 1-5A4. One monoclonal anti-idiotypic antibody (21-39) was directed against antibody 1-8D2 and six (CE1-3, CE2-1, CE7-2, CE8-46, CE9-35, and CE10-2) against antibody 3-12A1. The anti-idiotypic antibodies were all highly specific for the respective monoclonal antibody used for the induction of the anti-idiotype and did not cross-react with other monoclonal antibodies.
Hemmi
S
S
Fenner
M
M
Binz
H
H
Winterhalter
K
K
Wigzell
H
H
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis / Q000096:biosynthesis*; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000032:analysis / Q000096:biosynthesis*; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002459:Cell Fusion; D000939:Epitopes / Q000276:immunology; D006649:Histocompatibility Antigens / Q000276:immunology*; D007130:Immunoglobulin Idiotypes / Q000276:immunology* / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000939:Epitopes; D006649:Histocompatibility Antigens; D007130:Immunoglobulin Idiotypes; C038353:histocompatibility antigens RT, rat