Use of repetitive DNA probes as physical mapping strategy in Caenorhabditis elegans.
A method for linking genomic sequences cloned in yeast artificial chromosomes (YACs) has been tested using Caenorhabditis elegans as a model system. Yeast clones carrying YACs with repeated sequences were selected from a C. elegans genomic library, total DNA was digested with restriction enzymes, transferred to nylon membranes and probed with a variety of repetitive DNA probes. YAC clones that overlap share common bands with one or more repetitive DNA probes. In 159 YAC clones tested with one restriction enzyme and six probes 28 overlapping clones were detected. The advantages and limitations of this method for construction of YAC physical maps is discussed.
International Institute of Genetics and Biophysics, CNR, Naples, Italy.
G
G
Cangiano
H
H
Ameer
R
R
Waterston
A
A
La Volpe
D000818:Animals; D015139:Blotting, Southern; D002107:Caenorhabditis / Q000235:genetics*; D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D015342:DNA Probes; D015698:Genomic Library; D012091:Repetitive Sequences, Nucleic Acid
D015342:DNA Probes