A double antibody radioimmunoassay for human alpha 1-microglobulin.
Using purified alpha 1-microglobulin from urine and specific rabbit antiserum, a double antibody radioimmunoassay has been developed. The assay is sensitive to 3 ng/ml and covers a working range from 25-800 ng/ml. The recovery rates on the average were 91.8% in serum and 97.7% in urine. The coefficients of intra-assay variation ranged from 3.6-4.5% and those of inter-assay variation from 4.0-8.8%. Correlations in serum and urine between the present assay and single radial immunodiffusion (r = 0.972; r = 0.978) and enzyme-linked immunosorbent assay (r = 0.935; r = 0.971) were satisfactory.
Y
Y
Itoh
H
H
Nishino
H
H
Enomoto
T
T
Kawai
D000293:Adolescent; D000328:Adult; D000368:Aged; D000510:Alpha-Globulins / Q000032:analysis* / Q000276:immunology; D000818:Animals; D000906:Antibodies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D005779:Immunodiffusion / Q000379:methods; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods; D012016:Reference Values
D000510:Alpha-Globulins; D000906:Antibodies; C016001:alpha-1-microglobulin