Insulin, via its own receptor, regulates growth and amylase synthesis in pancreatic acinar AR42J cells.
Previous in vivo studies have suggested a long-term regulatory role for insulin in the exocrine pancreas. To directly study the long-term effects of insulin on the pancreas in vitro, we have used cultured AR42J cells, a rat cell line that is derived from a transplantable tumor of the acinar pancreas. Hormone-binding experiments with 125I-labeled hormones indicated that AR42J cells have insulin receptors, relatively fewer receptors for insulin-like growth factor II (IGF-II), and no detectable receptors for insulin-like growth factor I (IGF-I). Insulin at concentrations as low as 1 nM stimulated the growth of these cells, as measured by an increase in DNA and protein content, and in cell number. At 100 nM, where insulin had a maximal effect, the growth of AR42J cells was stimulated by 46.1 +/- 10.9% (mean +/- SEM, N = 11). Insulin increased the amylase activity of AR42J cells over the same concentration range that it stimulated growth; at 100 nM, insulin increased amylase by 91.0 +/- 15.4% (mean +/- SEM, N = 23). Immunoprecipitation of [35S]methionine-labeled proteins revealed that insulin induced a selective increase of amylase synthesis over general protein synthesis. These studies indicate, therefore, that insulin stimulates both growth and amylase synthesis of AR42J cells.
Mössner
J
J
Logsdon
C D
CD
Williams
J A
JA
Goldfine
I D
ID
D000681:Amylases / Q000096:biosynthesis*; D000818:Animals; D002460:Cell Line; D004247:DNA / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D007328:Insulin / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D010179:Pancreas / Q000032:analysis / Q000166:cytology* / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology; D011506:Proteins / Q000032:analysis; D051381:Rats; D011972:Receptor, Insulin / Q000378:metabolism / Q000502:physiology*; D013002:Somatomedins / Q000494:pharmacology
D007328:Insulin; D010455:Peptides; D011506:Proteins; D013002:Somatomedins; D004247:DNA; D011972:Receptor, Insulin; D000681:Amylases