Neutrophil depletion suppresses 111In-labeled platelet accumulation in infarcted myocardium.
Platelets and neutrophils accumulate rapidly in infarcted myocardium. Although antineutrophil agents reduce the size of the infarcted area, this is not observed with antiplatelet drugs. The possibility that myocardial ischemia-induced platelet deposition was secondary to a neutrophil-mediated event was assessed by injecting prostacyclin-washed autologous 111In-labeled platelets and measuring the amount of radioactivity in different regions of the heart following 90-min occlusion of the left anterior descending coronary artery followed by reperfusion for periods up to 5 h. Platelet deposition during the reperfusion phase was linear with time and similar to the time course of neutrophil accumulation. There was a transmural distribution of radioactivity across the myocardium where the "zone" between infarcted and risk regions, called the "interface," greater than infarct greater than risk greater than normal. Neutropenia (21 +/- 2% control levels), induced with specific sheep anti-dog neutrophil antiserum, had minimal effects on platelet aggregation ex vivo, but significantly reduced platelet accumulation in the ischemic myocardium following 5-h reperfusion and abolished the transmural platelet distribution. These results suggest that myocardial platelet deposition is secondary to a neutrophil-mediated event in this occlusion-reperfusion model of myocardial injury. Interactions between platelets and neutrophils at the site of tissue damage may influence the process of myocardial ischemic injury.
M
M
Bednar
B
B
Smith
A
A
Pinto
K M
KM
Mullane
D000818:Animals; D001792:Blood Platelets / Q000473:pathology*; D004285:Dogs; D007204:Indium; D007958:Leukocyte Count; D008297:Male; D009203:Myocardial Infarction / Q000097:blood* / Q000473:pathology; D009504:Neutrophils / Q000473:pathology*; D010976:Platelet Count; D011868:Radioisotopes; D013194:Staining and Labeling; D013997:Time Factors
D011868:Radioisotopes; D007204:Indium