Radiolabeling of monoclonal antibody against carcinoembryonic antigen with 88Y and biodistribution studies.
The biodistribution of the 202 monoclonal antibody against CEA labeled with 88Y by the bicyclic DTPA anhydride method was studied in normal Balb/c mice. The in vitro binding to 1 X 10(7) CO112, LS174T and WiDR colon cancer cells was 21.0, 27.3 and 18.8%, respectively. The binding to an equal number of KM-3 leukemia cells and normal human lymphocytes was 8.9 and 3.2%, respectively. Liver, spleen, kidney and blood were the tissues that showed the highest uptake of radiolabeled antibody in vivo.
D J
DJ
Buchsbaum
D E
DE
Hanna
B C
BC
Randall
F
F
Buchegger
J P
JP
Mach
D000818:Animals; D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen / Q000032:analysis* / Q000276:immunology; D002460:Cell Line; D003110:Colonic Neoplasms; D000939:Epitopes / Q000032:analysis; D007118:Immunoassay; D007700:Kinetics; D007938:Leukemia; D051379:Mice; D008807:Mice, Inbred BALB C; D014018:Tissue Distribution; D015021:Yttrium Radioisotopes
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D000939:Epitopes; D015021:Yttrium Radioisotopes