Transcription initiation complexes and upstream activation with RNA polymerase II lacking the C-terminal domain of the largest subunit.
RNA polymerase II assembles with other factors on the adenovirus type 2 major late promoter to generate pairs of transcription initiation complexes resolvable by nondenaturing gel electrophoresis. The pairing of the complexes is caused by the presence or absence of the C-terminal domain of the largest subunit. This domain is not required for transcription stimulation by the major late transcription factor in vitro.
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
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Buratowski
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Sharp
D000595:Amino Acid Sequence; D002918:Chymotrypsin / Q000494:pharmacology; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D012319:RNA Polymerase II / Q000378:metabolism*; D013329:Structure-Activity Relationship; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
D046911:Macromolecular Substances; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D012319:RNA Polymerase II; D002918:Chymotrypsin