Scanning electron microscope cytochemistry of blood cells.
The backscattered electron imaging (BEI) mode of scanning electron microscopy (SEM) has been applied to study various histo-cytochemical reactions in biological specimens since the early seventies. Due to numerous, recent technical improvements the BEI mode of SEM now belongs to the routine of many SEM laboratories. For cytochemistry, BEI has been mainly used to: visualize intracellular structures and organelles; recognize the different cell types in heterogeneous populations or tissues; study the correlations between enzymatic activities and cell surface features. We have evaluated the most relevant results obtained in the study of blood cells and the possible future applications of these techniques.
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D
Soligo
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de Harven
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Pozzoli
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MT
Nava
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Polli
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Lambertenghi-Deliliers
D001773:Blood Cells / Q000032:analysis* / Q000145:classification / Q000648:ultrastructure; D002462:Cell Membrane / Q000648:ultrastructure; D006402:Hematologic Diseases / Q000473:pathology; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D007948:Leukemia, Monocytic, Acute / Q000473:pathology; D007962:Leukocytes / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D013154:Spleen / Q000648:ultrastructure; D013194:Staining and Labeling / Q000379:methods