[Apurinic-apyrimidinic DNA-endonuclease activity of cytochrome c and pancreatic RNAse].
Cationic proteins--cytochrome c and pancreatic RNAase--possess the apurinic-apyrimidinic DNA-endonuclease activity. The affinity of these proteins for DNA-apurinic sites does not differ from that of specific apurinic DNA-endonucleases described in literature. The main features of the apurinic activity of cationic proteins are as follows: low specific activity, high temperature optimum of the reaction, absence of primer-stimulated activity. The feasibility of participation of cationic proteins and some other nucleophilic compounds in single-stranded breaks production in apurinic DNA is discussed.
O K
OK
Kaboev
L A
LA
Luchkina
M L
ML
Bekker
D000818:Animals; D001080:Apurinic Acid / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism*; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D043223:Deoxyribonuclease IV (Phage T4-Induced); D004591:Electrophoresis, Polyacrylamide Gel; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D006736:Horses; D006868:Hydrolysis; D066298:In Vitro Techniques; D010179:Pancreas / Q000201:enzymology; D010957:Plasmids; D011119:Polynucleotides / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism*; D013379:Substrate Specificity
D004277:DNA, Single-Stranded; D011119:Polynucleotides; C001790:apyrimidinic acid; D001080:Apurinic Acid; D004706:Endodeoxyribonucleases; D012260:Ribonucleases; D043223:Deoxyribonuclease IV (Phage T4-Induced); D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase
Apurin-apirimidinovaia DNK-éndonukleaznaia aktivnost' tsitokhroma c i pankreaticheskoĭ RNKazy.