[Effect of non-enzymatic desamidation of gamma-globulin on its properties and biological activity].
During incubation of gamma-globulin preparations under conditions similar to physiological ones within 28 days (0.9% NaCl, pH 7.0, 37 degrees) as well as during storage at 3-10 degrees within 36 months gradual nonenzymatic deamidation of the protein occurred mainly due to hydrolysis of readily accessible amide groups (asparagine). Fluorescence of the preparations decreased gradually in the course of deamidation; it constituted for 82% of the initial value after incubation during 28 days. Immunological activity of the gamma-globulin, which lost 19.6% of amide groups, was decreased by 34%; alteration in the amidation by 45.1% was accompanied by a decrease in active antibodies concentration by 92.3%. Nonenzymatic deamidation may be responsible for conformational alterations and inactivation of gamma-globulin.
Lukash
A I
AI
Shepelev
A P
AP
Pushkina
N V
NV
Tsybul'skiĭ
I E
IE
Al'perovich
D V
DV
D000577:Amides / Q000032:analysis; D000641:Ammonia / Q000032:analysis; D003641:Deamination; D004355:Drug Stability; D004356:Drug Storage; D006801:Humans; D007116:Immunization, Passive; D008457:Measles / Q000517:prevention & control; D011233:Precipitin Tests; D011487:Protein Conformation; D005719:gamma-Globulins / Q000032:analysis* / Q000276:immunology
D000577:Amides; D005719:gamma-Globulins; D000641:Ammonia
Vliianie nefermentativnogo dezamidirovaniia gamma-globulina na ego svoĭstva i biologicheskuiu aktivnost'.