Structural basis for the species selectivity of a fibrin-specific monoclonal antibody.
The structural determinant underlying the species specificity of a monoclonal anti-fibrin antibody (59D8) is the leucyl residue at position 5 in beta-chains of human fibrin. Anti-fibrin antibody 59D8 which had been elicited by immunization with human beta(1-7) peptide, Gly-His-Arg-Pro-Leu-Asp-Lys, binds to human and canine fibrins but not to bovine, ovine, or porcine fibrins. A comparison of the available amino acid sequence data suggested that the ability of anti-fibrin antibody 59D8 to discriminate among various fibrin beta-chains might be due to the amino acid at position 5. This was confirmed by competitive inhibition studies using synthetic fibrin-like peptides and determination of the amino acid sequences of the N-termini of ovine and porcine fibrin beta-chains. Edman degradation employing o-phthalaldehyde blocking permitted use of fibrin monomer rather than its separated constituent polypeptide chains. The same sequencing strategy was used to obtain partial sequence data for the alpha-chains of bovine, ovine, and porcine fibrin.
G R
GR
Matsueda
M N
MN
Margolies
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D002417:Cattle; D004285:Dogs; D000939:Epitopes / Q000032:analysis; D005337:Fibrin / Q000032:analysis / Q000276:immunology*; D006801:Humans; D007202:Indicators and Reagents; D009842:Oligopeptides / Q000138:chemical synthesis; D012756:Sheep; D013045:Species Specificity; D013552:Swine
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000939:Epitopes; D007202:Indicators and Reagents; D009842:Oligopeptides; D005337:Fibrin