Local base dynamics and local structural features in RNA and DNA duplexes.
Local base motion and local structural base information are derived with a simple motional model from site specifically spin-labeled polyribo- and polydeoxyribonucleotides. The model was developed earlier for some nucleic acids and has now been applied to analyze 22 different nucleic acid systems. We conclude that the base motion of the spin-labeled nucleotide in single-stranded RNA, DNA, or non-base-paired bases in duplexes is of the order of 1 ns and that its base mobility decreases by about a factor of 4 upon base pairing. Also, the tether motion of the probe is slower in an RNA than in a DNA duplex.
Kao
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Bobst
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D004247:DNA; D004578:Electron Spin Resonance Spectroscopy; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D011089:Polydeoxyribonucleotides; D011131:Polyribonucleotides; D012313:RNA; D013113:Spin Labels; D013329:Structure-Activity Relationship
D011089:Polydeoxyribonucleotides; D011131:Polyribonucleotides; D013113:Spin Labels; D012313:RNA; D004247:DNA