Measurement of 5-aminolaevulinate synthase activity by gas-liquid chromatography with electron-capture detection.
A specific assay for 5-aminolaevulinate synthase activity is described, with a sensitivity comparable with that of radiochemical assays. It is based on measurement by g.l.c. with electron-capture detection of the pentafluorobenzyl ester of the ethyl acetoacetate pyrrole derivative of 5-aminolaevulinic acid, and of the corresponding compound from 6-amino-5-oxohexanoic acid used as internal standard. Enzyme activity has been measured in homogenates of rat liver, spleen, kidney and brain, and in human lymphocytes.
Gorchein
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Department of Clinical Pharmacology, St. Mary's Hospital Medical School, London, U.K.
D000624:5-Aminolevulinate Synthetase / Q000032:analysis*; D000818:Animals; D001921:Brain / Q000201:enzymology; D002849:Chromatography, Gas / Q000379:methods; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008214:Lymphocytes / Q000201:enzymology; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains; D013154:Spleen / Q000201:enzymology
D000624:5-Aminolevulinate Synthetase