The T-cell-independent immune response to the hapten NP uses a large repertoire of heavy chain genes.
Hybridomas generated from C57BL/6 mice immunized with the hapten NP coupled to ficoll, a T-cell-independent carrier, produce monoclonal antibodies that use a large repertoire of VH regions and light chains. This contrasts with the homogeneity of the strain-specific response to NP observed with T-cell-dependent carriers, where most of the antibodies use a single VH region, V186.2, in combination with the lambda-1 light chain. There is no evidence for somatic mutation in any of the sequenced regions of the antibodies generated by NP-ficoll. Thus T cell participation is required for the homogeneity of the strain-specific hapten response, and probably for somatic mutation as well.
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Maizels
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Bothwell
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D001483:Base Sequence; D002645:Chickens / Q000276:immunology; D005362:Ficoll / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL / Q000276:immunology; D009596:Nitrophenols / Q000276:immunology*; D010648:Phenylacetates; D013601:T-Lymphocytes; D005719:gamma-Globulins / Q000276:immunology
D000911:Antibodies, Monoclonal; D007143:Immunoglobulin Heavy Chains; D009596:Nitrophenols; D010648:Phenylacetates; D005719:gamma-Globulins; C026242:4-hydroxy-5-nitrophenyl acetic acid; D005362:Ficoll