Effect of glycerol on cell kinetics and tumorigenesis in mouse lung following urethan administration.
Extending previous studies, we hypothesized that glycerol modulates pulmonary tumorigenesis in mice through metabolic activation in bronchiolar cells. To test this, we examined the effects of glycerol on pulmonary cell kinetics and tumorigenesis induced by urethan. Male ddY mice were given 1 mg/g urethan and/or 5% glycerol solution for a fixed period. [3H]Thymidine autoradiography revealed that glycerol administration significantly suppressed the urethan-induced facilitation of cell kinetics of the bronchiolar cells (p less than 0.05), but not that of the alveolar wall cells. Furthermore, glycerol did not affect urethan-induced pulmonary tumorigenesis. These findings suggest that glycerol modifies the metabolism of carcinogens in the bronchiolar epithelium.
Nagahara
N
N
Department of Pathology, Yokohama City University School of Medicine, Japan.
Kitamura
H
H
Inoue
T
T
Ogawa
T
T
Ito
T
T
Kanisawa
M
M
D000818:Animals; D001980:Bronchi / Q000187:drug effects / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D004247:DNA / Q000378:metabolism; D004848:Epithelium / Q000187:drug effects / Q000473:pathology; D005990:Glycerol / Q000494:pharmacology*; D007399:Interphase; D008175:Lung Neoplasms / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D011650:Pulmonary Alveoli / Q000187:drug effects / Q000473:pathology; D013936:Thymidine / Q000378:metabolism; D014316:Tritium / Q000378:metabolism; D014520:Urethane / Q000008:administration & dosage / Q000633:toxicity*
D014316:Tritium; D014520:Urethane; D004247:DNA; D005990:Glycerol; D013936:Thymidine