Effect of polyethylene glycol on the non-specific adsorption of proteins to Eupergit C and agarose.
Non-specific adsorption of serum proteins to Eupergit C (EC) and agarose during the process of immunoaffinity chromatography often leads to contamination of the specifically eluted antigens to be purified. This effect was studied by application of serum samples to a beta-mercaptoethanol-blocked EC (EC-beta ME) column followed by analysis of proteins eluted with various elution buffers. Inclusion of polyethylene glycol (PEG 400 or 1500) in the loading buffer reduced the non-specific adsorption of proteins to EC but had an adverse effect on agarose. Covalent attachment of amino-PEG to EC and to epoxy-activated Sepharose mimicked the effect of PEG in solution with EC and resulted in a marked reduction in non-specific adsorption of serum proteins. Inclusion of PEG in the loading buffer during immunopurification of a serum protein (immunoglobulin G) or seminal plasma protein (human decidua protein hDP71) resulted in a marked improvement in the purity of these proteins eluted from the respective columns by ammonium acetate (pH 10).
Fleminger
G
G
Department of Biotechnology, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Israel.
Solomon
B
B
Wolf
T
T
Hadas
E
E
D000327:Adsorption; D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D006801:Humans; D007120:Immunochemistry; D007074:Immunoglobulin G / Q000302:isolation & purification; D011092:Polyethylene Glycols; D012685:Sepharose
D001798:Blood Proteins; D007074:Immunoglobulin G; D011092:Polyethylene Glycols; D012685:Sepharose