Calibration by ellipsometry of the enzyme-linked immunosorbent assay.
The enzyme-linked immunosorbent assay (ELISA) was analyzed with regard to possible diffusion limitations of the binding reaction. The absorbance values of the assay were found to follow the time and concentration relations that would occur when diffusion of antibody to the surface is the rate limiting step. This relationship was used in order to calibrate the absorbance values of the ELISA with antibody concentration by ellipsometry, which itself allows direct measurement of the amount of antibody bound to the solid phase.
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Nygren
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Stenberg
D000818:Animals; D000918:Antibody Specificity; D002417:Cattle; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D007700:Kinetics; D011817:Rabbits
D000939:Epitopes