The effect of sodium hyaluronate on the migration of rabbit corneal epithelium. I. An in vitro study.
The effect of sodium hyaluronate (Na-HA) on the migration of corneal epithelium was investigated in vitro using corneo-scleral sections of rabbits. The effect on migration was evaluated by the length of migrating epithelium on the cut surface of corneal stroma after incubation in media containing Na-HA of various molecular weights or concentrations. The medium concentration of Na-HA was 400 micrograms/ml, and the molecular weights were 0.4 x 10(4), 2 x 10(4), 10 x 10(4), 68 x 10(4), 102 x 10(4) and 216 x 10(4). Regarding Na-HA with a molecular weight of 102 x 10(4); media containing Na-HA at concentrations of 50, 100, 200, 400, 800, 2000 and 4000 micrograms/ml were also investigated. When the concentration of Na-HA was 400 micrograms/ml, Na-HA with molecular weights of over 68 x 10(4) accelerated significantly the epithelial migration. Regarding Na-HA with a molecular weight of 102 x 10(4), acceleration was observed at concentrations of 200, 400 and 800 micrograms/ml. Furthermore, the concentration of fibronectin (FN) in medium containing the effective Na-HA was significantly lower than that in the control medium after the incubation. These results suggest a possibility that the accelerative effect of Na-HA depends on the reduction of FN release from the cornea or the facilitation of FN deposition on the cornea.
Tokyo Research Institute, Seikagaku-Kogyo Co., Ltd., Japan.
S
S
Miyauchi
T
T
Sugiyama
A
A
Machida
T
T
Sekiguchi
K
K
Miyazaki
K
K
Tokuyasu
K
K
Nakazawa
D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D003315:Cornea / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D003319:Corneal Stroma / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D005353:Fibronectins / Q000378:metabolism / Q000494:pharmacology; D006820:Hyaluronic Acid / Q000494:pharmacology*; D066298:In Vitro Techniques; D008970:Molecular Weight; D011817:Rabbits
D005353:Fibronectins; D004815:Epidermal Growth Factor; D006820:Hyaluronic Acid