Effect of somatostatin and its analogs on histamine-stimulated cAMP production in isolated guinea pig gastric glands.
The effect of somatostatin-14 (ss-14), somatostatin-28 (ss-28), and [D-trp8]somatostatin-14 ([ D-trp8]ss-14) on both histamine-stimulated cellular cAMP production and [3H]-cimetidine binding on plasma membranes in isolated guinea pig gastric glands was investigated. These three peptides partially inhibited (approximately 50% maximally) the increase of cAMP production stimulated by histamine. There was no inhibition of [3H]-cimetidine binding on plasma membranes from these isolated gastric glands. These results indicate that somatostatin acts directly on parietal cells and inhibits histamine-stimulated cAMP production with no influence on H2 receptor in histamine stimulated gastric secretion. Inhibitory potency of somatostatin and its analogs against histamine-stimulation may be equal at the cellular level.
S
S
Nishihara
A
A
Tanaka
K
K
Yoshida
Y
Y
Imazono
T
T
Misawa
A
A
Funakoshi
H
H
Ibayashi
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002927:Cimetidine / Q000378:metabolism; D000242:Cyclic AMP / Q000096:biosynthesis*; D005753:Gastric Mucosa / Q000378:metabolism*; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D011970:Receptors, Histamine H2 / Q000187:drug effects; D013004:Somatostatin / Q000378:metabolism / Q000494:pharmacology*; D053561:Somatostatin-28
D011970:Receptors, Histamine H2; D013004:Somatostatin; D053561:Somatostatin-28; D002927:Cimetidine; D006632:Histamine; D000242:Cyclic AMP; D015056:1-Methyl-3-isobutylxanthine