Localization of axonally transported [125I]wheat germ agglutinin in rat abducens motoneuron axons and terminals after intracisternal injection.
Iodinated wheat germ agglutinin (WGA) was taken up and transported by rat abducens motoneurons to nerve terminals in the lateral rectus muscle. Five days after intracisternal injection of lectin, axon terminals wee found to be the most radioactive source, based on the density of labeling. In axons the radioactive label was concentrated in an annular region about 1.26 microns wide beneath the plasma membrane. We hypothesize that the WGA was associated with smooth vesicular and tubular structures in the axoplasm but not limited to organelles immediately beneath the axon plasma membrane. No evidence of intercellular transfer to muscle cells was found.
J H
JH
LaVail
D000010:Abducens Nerve / Q000378:metabolism* / Q000648:ultrastructure; D000818:Animals; D001370:Axonal Transport; D001369:Axons / Q000648:ultrastructure; D005260:Female; D037102:Lectins; D008297:Male; D008854:Microscopy, Electron; D009046:Motor Neurons / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D014909:Wheat Germ Agglutinins
D037102:Lectins; D014909:Wheat Germ Agglutinins