Complete nucleotide sequence of a gene coding for heat- and pH-stable alpha-amylase of Bacillus licheniformis: comparison of the amino acid sequences of three bacterial liquefying alpha-amylases deduced from the DNA sequences.
The gene coding for the heat-stable and pH-stable alpha-amylase of Bacillus licheniformis 584 (ATCC 27811) was cloned in Escherichia coli and the nucleotide sequence of a DNA fragment of 1,948 base pairs containing the entire amylase gene was determined. As inferred from the DNA sequence, the B. licheniformis alpha-amylase had a signal peptide of 29 amino acid residues and the mature enzyme comprised 483 amino acid residues, giving a molecular weight of 55,200. The amino acid sequence of B. licheniformis alpha-amylase showed 65.4% and 80.3% homology with those of heat-stable Bacillus stearothermophilus alpha-amylase and relatively heat-unstable Bacillus amyloliquefaciens alpha-amylase, respectively. Nevertheless, several regions of the alpha-amylases appeared to be clearly distinct from one another when their hydropathy profiles were compared.
T
T
Yuuki
T
T
Nomura
H
H
Tezuka
A
A
Tsuboi
H
H
Yamagata
N
N
Tsukagoshi
S
S
Udaka
D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D006863:Hydrogen-Ion Concentration; D010957:Plasmids; D012329:RNA, Bacterial / Q000235:genetics; D013696:Temperature; D000516:alpha-Amylases / Q000235:genetics*
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D004262:DNA Restriction Enzymes; D000516:alpha-Amylases