[Are the regulatory cation-binding sites of opiate receptors a part of potential-dependent calcium channels of neuromembranes?].
Characteristics of 45Ca2+ transport into rat brain synaptosomes have been studied. The value of an equilibrium constant of the Ca2+ binding at a specific site of neuromembrane's potential-stimulated calcium channel was determined (KM = 0,27 +/- 0,04 mM). Stability constants of several metal-ion complexes with the cation-binding regulatory site at high- and low-affinity receptors for [D-Ala2, D-Leu5]enkephaline and morphine high-affinity receptors of rat brain membranes were estimated. Comparative analysis of the constants calculated along with the results of investigation of a calcium antagonist, Verapamil, and Zn2+ ions influence on various types of opiate receptors allow to conclude that regulatory cation-binding sites of enkephaline and morphine receptors are not identical to Ca2+-binding centres of potential-stimulated calcium channels of the neuromembrane.
Porodenko
N V
NV
Zaĭtsev
S V
SV
Varfolomeev
S D
SD
D000818:Animals; D001665:Binding Sites; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D066298:In Vitro Techniques; D007473:Ion Channels / Q000378:metabolism*; D007700:Kinetics; D008670:Metals / Q000378:metabolism; D051381:Rats; D011957:Receptors, Opioid / Q000378:metabolism*; D013570:Synaptic Membranes / Q000378:metabolism*
D007473:Ion Channels; D008670:Metals; D011957:Receptors, Opioid; D002118:Calcium
Iavliaiutsia li reguliatornye kationsviazyvaiushchie tsentry opiatnykh retseptorov chast'iu potentsialzavisimykh kal'tsievykh kanalov neĭromembrany?