[Sequential changes in the macrostructure and RNA-synthesizing activity of nucleolar and extranucleolar chromatin from rat liver cells during induction of DNA synthesis].
The dynamics of structural changes and RNA-polymerase activity in rat liver cell chromatin caused by drastic changes in the rates of protein synthesis was investigated. Inhibition of protein synthesis after a single injection of animals with cycloheximide (0.3 mg/100 g of body weight) increased the total condensibility of chromatin. Under these conditions, the stepwise activation of RNA-polymerases I and II correlated with decondensation of chromatin. By the 6-12th hour following cycloheximide injection, a chromatin fraction enriched with RNA-polymerase I and a RNA-polymerase II-rich fraction could be isolated from liver cell nuclei.
Shevchenko
N A
NA
Boĭkov
P Ia
PIa
Todorov
I N
IN
D000818:Animals; D002466:Cell Nucleolus / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000096:biosynthesis; D003513:Cycloheximide / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D009994:Osmolar Concentration; D011487:Protein Conformation; D012313:RNA / Q000096:biosynthesis*; D051381:Rats; D013997:Time Factors
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D012313:RNA; D004247:DNA; D003513:Cycloheximide; D012321:DNA-Directed RNA Polymerases
Posledovatel'nye izmeneniia makrostrukturnogo sostoianiia i RNK-sinteziruiushcheĭ aktivnosti iadryshkovogo i ékstraiadryshkovogo khromatina kletok pecheni krys v protsesse induktsii sinteza DNK.