Binding of 3H-iloprost to rat gastric mucosa: a pitfall in performing radioligand binding assays.
Binding of 3H-iloprost was studied in a 20,000 x g sediment of the rat gastric mucosa. When pH in both test tubes for total and non-specific binding was kept identical, no displaceable binding of iloprost could be detected. When no care was taken to keep the pH identical in corresponding test tubes of the binding assay, changes in pH simulated specific and displaceable binding of iloprost. Therefore it is concluded that - in contrast to earlier reports - it is not possible to demonstrate specific iloprost binding using the given method.
Beinborn
M
M
Kromer
W
W
Staar
U
U
Sewing
K F
KF
D000818:Animals; D001665:Binding Sites; D011464:Epoprostenol / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D016285:Iloprost; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D011869:Radioligand Assay; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium
D014316:Tritium; D011464:Epoprostenol; D016285:Iloprost