Mediation of anion transport in oocytes of Xenopus laevis by biosynthetically inserted band 3 protein from mouse spleen erythroid cells.
mRNA from the spleens of anemic mice was purified by oligo(dT)-cellulose chromatography and fractionated by density gradient centrifugation. After injection into oocytes of Xenopus laevis, two of the four fractions obtained led, after 16 h of incubation at 20 degrees C, to the expression of mouse band 3 protein, as demonstrated by immunoprecipitation with polyclonal antibodies against mouse band 3. Flux measurements showed an approximately 2- to 4-fold increment of 36Cl- uptake, which could be abolished by two different stilbene disulfonates, specific inhibitors of band 3-mediated anion transport in red blood cells.
M
M
Morgan
P
P
Hanke
R
R
Grygorczyk
A
A
Tintschl
H
H
Fasold
H
H
Passow
D000818:Animals; D001457:Anion Exchange Protein 1, Erythrocyte / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D009865:Oocytes / Q000378:metabolism*; D011061:Poly A / Q000235:genetics; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D013154:Spleen / Q000378:metabolism; D014981:Xenopus
D001457:Anion Exchange Protein 1, Erythrocyte; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA