Presumptive identification of Clostridium difficile by detection of p-cresol in prepared peptone yeast glucose broth supplemented with p-hydroxyphenylacetic acid.
Prereduced, anaerobically sterilized peptone yeast glucose broth was supplemented with p-hydroxyphenylacetic acid and used for the presumptive identification of Clostridium difficile. Two hundred eighty-two organisms were grown in this medium for 18 h and tested for p-cresol production by gas-liquid chromatography. All 49 stock and reference strains of C. difficile and 19 organisms confirmed as C. difficile produced p-cresol. p-Cresol was not produced by 53 negative control or 161 test organisms. The system was convenient and effective.
Department of Laboratory Medicine, Calgary General Hospital, Alberta, Canada.
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Sivsammye
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D002849:Chromatography, Gas; D003013:Clostridium / Q000302:isolation & purification*; D003408:Cresols / Q000378:metabolism*; D003470:Culture Media; D006801:Humans; D010648:Phenylacetates
D003408:Cresols; D003470:Culture Media; D010648:Phenylacetates; C032538:4-cresol; C008070:4-hydroxyphenylacetic acid