Tumor cells stimulate interleukin 1 (IL-1) production from enriched large granular lymphocytes.
When enriched large granular lymphocytes (LGLs) were activated by K562 cells substantial amounts of interleukin 1 (IL-1) could be detected in the supernatants as measured by the mouse thymocyte assay. IL-1 could also be produced by LGLs treated with other tumor cells according to their ability to be lysed by LGLs. Therefore PLC/PRF/5 hepatoma cells which were moderately sensitive to LGL attack stimulated moderate amounts of IL-1 from the LGLs. Yac-1 cells and human fibroblasts which are resistant to LGL cytolysis did not activate LGLs to produce significant amounts of IL-1. IL-1 was shown to be produced by LGLs and not by the stimulatory tumor cells. Interferon-alpha (IFN), which reduced the susceptibility of target cells to be lysed by LGLs, also inhibited their ability to stimulate IL-1 production from LGLs. The IL-1 stimulatory effect of tumor cells on LGLs could not be attributed to Mycoplasma or endotoxin contamination. It is suggested that LGLs release IL-1 when they encounter susceptible cells and that release of this cytokine is important in the subsequent lysis of target cells.
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Herman
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AR
Rabson
D000818:Animals; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007372:Interferons / Q000494:pharmacology; D007375:Interleukin-1 / Q000096:biosynthesis*; D007694:Killer Cells, Natural / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D009369:Neoplasms / Q000276:immunology / Q000378:metabolism*; D009439:Neuraminidase / Q000494:pharmacology; D011112:Polymyxin B / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology
D007375:Interleukin-1; D008070:Lipopolysaccharides; D007372:Interferons; D003513:Cycloheximide; D009439:Neuraminidase; D014357:Trypsin; D011112:Polymyxin B