[Clinical experiences with in vitro testing of chemotherapeutic agents].
The response of tumour cells from 25 tumours (mainly squamous cell carcinomas) to Cisplatinum, Bleomycin and Methotrexate in the stem-cell cloning assay described by Hamburger and Salmon was compared to the response to combination therapy with Bleomycin, Methotrexate and Cisplatinum. In 90%, the result of the stem-cell assay yielded a correct prediction of the chemosensitivity or resistance of the tumour within 9 weeks. This test predicts with high probability the effectiveness of chemotherapeutic agents long before the clinical result becomes manifest. Thus, the application of pre-operative chemotherapy becomes easier. The possibility of predicting the ineffectiveness of cytostatic agents spares the patient the often considerable side effects to a treatment whose ineffectiveness until now only became apparent after a prolonged period.
Axhausen
M
M
Matthias
R
R
D000970:Antineoplastic Agents / Q000494:pharmacology*; D001761:Bleomycin / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects*; D002945:Cisplatin / Q000494:pharmacology; D003114:Colony-Forming Units Assay; D004351:Drug Resistance; D006801:Humans; D008727:Methotrexate / Q000494:pharmacology; D010039:Otorhinolaryngologic Neoplasms; D014410:Tumor Stem Cell Assay
D000970:Antineoplastic Agents; D001761:Bleomycin; D002945:Cisplatin; D008727:Methotrexate
Klinische Erfahrungen mit der in-vitro-Testung von Chemotherapeutika.