Immunoenzymatic staining of viral and chlamydial antigens in cell culture.
Methods are described for the conjugation of antibodies with biotin and for the use of these reagents in an immunoperoxidase staining procedure for infected cell cultures. This technique provides a simple, rapid, and specific approach to the identification and characterization of a number of viral and chlamydial isolates in the diagnostic laboratory.
Richman
D D
DD
Cleveland
P H
PH
Wopschall
L J
LJ
D000256:Adenoviridae / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology; D001710:Biotin; D002460:Cell Line; D002692:Chlamydia trachomatis / Q000276:immunology*; D003587:Cytomegalovirus / Q000276:immunology; D004285:Dogs; D000939:Epitopes; D014645:Herpesvirus 3, Human / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D009980:Influenza A virus / Q000276:immunology; D009981:Influenza B virus / Q000276:immunology; D018139:Simplexvirus / Q000276:immunology; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000956:Antigens, Viral; D000939:Epitopes; D001710:Biotin