Ultrastructural effects of recombinant gamma-interferon on cultured human keratinocytes.
To extend our initial observations that recombinant gamma-interferon (r gamma IFN) influences the growth and differentiation of normal cultured human keratinocytes, we studied the electron microscopic changes induced by r gamma IFN. Treatment of cultured human keratinocytes with 10(3) units/ml (7.1 nM) of r gamma IFN produced a shift toward an increasing percentage of attached cells that had a mature-type, differentiated appearance rather than a basal type of proliferating cell, as observed in control, untreated cultures. This report extends the number of cell types that can be influenced by r gamma IFN at the ultrastructural level and supports the notion that r gamma IFN can influence both growth and maturation of normal cultured human keratinocytes.
B J
BJ
Nickoloff
G
G
Mahrle
V
V
Morhenn
D002478:Cells, Cultured; D000078404:Epidermal Cells; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D007633:Keratins / Q000276:immunology / Q000494:pharmacology*; D008854:Microscopy, Electron; D011994:Recombinant Proteins / Q000494:pharmacology*
D011994:Recombinant Proteins; D007633:Keratins; D007371:Interferon-gamma