Flow-cytometric determination of glutathione alterations in vital cells by o-phthaldialdehyde (OPT) staining.
A flow-cytometric method for the detection of changes of cellular glutathione content in vital cells is described. The reaction is based on formation of a fluorescent product between o-phthaldialdehyde (OPT) and reduced glutathione (GSH). OPT is a more GSH-specific dye than other thiol-specific dyes (e.g., bromobimanes), because it forms a cyclic compound with GSH. Changes of GSH induced by oxidation or thiol-blocking agents are visualized in vital cells after a 5-min staining at room temperature.
Treumer
J
J
Valet
G
G
D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000473:pathology; D002851:Chromatography, High Pressure Liquid; D005434:Flow Cytometry / Q000379:methods; D005978:Glutathione / Q000378:metabolism*; D051379:Mice; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling; D009764:o-Phthalaldehyde / Q000494:pharmacology*
D000447:Aldehydes; D009764:o-Phthalaldehyde; D005978:Glutathione