Expression of HLA class I alpha chain determinants by human X mouse hybrid T cells is correlated with HLA-beta 2m but not with H-2.
The expression of HLA class I alpha chains by clones derived from a human X mouse T-cell line containing human chromosomes 6 and 15 was analysed by immunofluorescence. Evidence is presented showing that the expression of HLA class I alpha chains is associated with HLA-beta 2m. This is based on the phenotypic and quantitative fluorescence flow cytometric analysis of a series of cloned hybrids. In contrast, HLA and H-2 class I determinants show no evidence of such correlation. Results of cell-sorting indicate that the loss of HLA class I alpha chain expression is due to phenotypic modulation, in addition to gene segregation.
Durrant
L
L
Taylor
G M
GM
Parkar
M
M
Elles
R
R
Harris
R
R
D000818:Animals; D000911:Antibodies, Monoclonal; D002469:Cell Separation; D000939:Epitopes; D005434:Flow Cytometry; D006183:H-2 Antigens / Q000276:immunology*; D006680:HLA Antigens / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology*; D046911:Macromolecular Substances; D051379:Mice; D013045:Species Specificity; D001613:beta 2-Microglobulin / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006183:H-2 Antigens; D006680:HLA Antigens; D000949:Histocompatibility Antigens Class II; D046911:Macromolecular Substances; D001613:beta 2-Microglobulin