Sweat protein components tested by SDS-polyacrylamide gel electrophoresis followed by immunoblotting.
Sweat samples were collected in a sauna from 74 healthy volunteers (72 men and 2 women) and concentrated. SDS-polyacrylamide gel electrophoresis (SDS-PAGE) of the individual samples revealed, in general, five main proteins and four PAS positive components. In pooled sweat, a method of SDS-PAGE followed by immunoblotting with specific antisera or antibodies against 24 human serum components was applied, and three out of the five main proteins showed the same molecular weights and antigenicities corresponding to serum albumin (67,000 Da), Zn-alpha 2-glycoprotein (42,000 Da) and lysozyme (14,000 Da). Moreover, orosomucoid, transferrin, IgG and IgA were demonstrated in the pooled sweat. Although alpha 1-antitrypsin was probably in the pooled sweat, other serum components could not be detected. On the pooled and individual sweat samples, anti-carcinoembryonic antigen (CEA) formed three bands at 42,000, 19,000 and 18,000 Da, but the antibody did not react with normal serum. It might be considered from these molecular weights that those sweat components are CEA-related antigens.
Department of Legal Medicine, Iwate Medical University School of Medicine, Morioka.
N
N
Nakayashiki
D000328:Adult; D002272:Carcinoembryonic Antigen / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006023:Glycoproteins / Q000302:isolation & purification; D006801:Humans; D015151:Immunoblotting; D007136:Immunoglobulins / Q000302:isolation & purification; D008297:Male; D008970:Molecular Weight; D009113:Muramidase / Q000302:isolation & purification; D011506:Proteins / Q000276:immunology / Q000302:isolation & purification*; D029589:Seminal Plasma Proteins; D012709:Serum Albumin / Q000302:isolation & purification; D012967:Sodium Dodecyl Sulfate; D013542:Sweat / Q000032:analysis* / Q000276:immunology; D000090203:Zn-Alpha-2-Glycoprotein
D002272:Carcinoembryonic Antigen; D006023:Glycoproteins; D007136:Immunoglobulins; D011506:Proteins; D029589:Seminal Plasma Proteins; D012709:Serum Albumin; D000090203:Zn-Alpha-2-Glycoprotein; D012967:Sodium Dodecyl Sulfate; D009113:Muramidase