Mechanism of interferon action--the discovery of the pppA2'p5'A2'p5'A receptor.
In this paper it is demonstrated that (3H)2'-5'P3A3 can bind to the peritoneal macrophages from Wistar rat. This binding is strongly inhibited by cold 2'-5'P3A3, while the inhibiting capacity of 2'-5'P3I3 and 2'-5'A3 is very small, showing that this binding is highly specific. And the binding of (3H)2'-5'P3A3 is reversible, saturable and with high affinity. A Scatchard analysis of the binding data gives a linear plot, an apparent dissociation constant (Kd) about 1.3 X 10(-7) M and binding sites per cell about 2.1 X 10(7). The above evidence shows the existence of the 2'-5'P3A3 receptor, a fact which, to our knowledge, has not been reported before.
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D015088:2',5'-Oligoadenylate Synthetase / Q000276:immunology; D000227:Adenine Nucleotides / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000276:immunology; D000818:Animals; D001665:Binding Sites; D007369:Interferon Inducers / Q000276:immunology; D007372:Interferons / Q000276:immunology / Q000494:pharmacology*; D008264:Macrophages / Q000276:immunology / Q000378:metabolism*; D008297:Male; D009843:Oligoribonucleotides / Q000378:metabolism*; D010587:Phagocytosis; D011869:Radioligand Assay; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000276:immunology*; D011955:Receptors, Drug / Q000378:metabolism*; D011983:Receptors, Purinergic
D000227:Adenine Nucleotides; D007369:Interferon Inducers; D009843:Oligoribonucleotides; D011956:Receptors, Cell Surface; D011955:Receptors, Drug; D011983:Receptors, Purinergic; C048575:pppA2'p5'A2'p5'A receptor; C016964:adenosine triphosphate adenosine monophosphate adenosine monophosphate; D000255:Adenosine Triphosphate; D007372:Interferons; D015088:2',5'-Oligoadenylate Synthetase