Cross-linking of immunoglobulin E-receptor complexes induces their interaction with the cytoskeleton of rat basophilic leukemia cells.
Bridging of immunoglobulin E (IgE)-receptor complexes on rat basophilic leukemia cells by polyclonal anti-IgE antibodies induces a detergent-resistant association of these complexes with the cellular cytoskeleton. In dose-response curves the extent of the cytoskeletal association appears to follow the extent of bridging, continuing to increase beyond where stimulated degranulation is maximal. This stable association is maintained after the aggregated IgE-receptor complexes have been internalized by the cell. Multivalent antigen and trimeric IgE cause less extensive receptor cross-linking than anti-IgE and stimulate degranulation; they also induce receptor association with the cytoskeleton that is revealed only after stabilization by addition of a chemical cross-linking reagent. The ability of a membrane impermeant chemical cross-linker to stabilize this association suggests that the receptor-cytoskeletal interaction may be mediated by a transmembrane protein that is exposed at the cell surface. Monomeric and dimeric IgE bound to receptors fail to induce a stable interaction with the cytoskeleton even in the presence of chemical cross-linkers and are poor (dimers) or insignificant (monomers) stimulators of cellular degranulation. These findings are consistent with a possible relationship between receptor attachment to the cytoskeleton, receptor immobilization as measured by fluorescence photobleaching recovery, and the triggering of cellular degranulation.
Robertson
D
D
Holowka
D
D
Baird
B
B
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D001491:Basophils / Q000276:immunology / Q000378:metabolism*; D003432:Cross-Linking Reagents; D003599:Cytoskeleton / Q000276:immunology / Q000378:metabolism*; D004136:Dinitrobenzenes / Q000276:immunology; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D006636:Histamine Release; D007073:Immunoglobulin E / Q000276:immunology / Q000378:metabolism*; D007942:Leukemia, Experimental / Q000276:immunology / Q000378:metabolism*; D017830:Octoxynol; D011092:Polyethylene Glycols; D051381:Rats; D011961:Receptors, Fc / Q000378:metabolism*; D017455:Receptors, IgE; D011971:Receptors, Immunologic / Q000378:metabolism*; D012995:Solubility; D013388:Succinimides; D013861:Thiocyanates
D000888:Antibodies, Anti-Idiotypic; D003432:Cross-Linking Reagents; D004136:Dinitrobenzenes; D005452:Fluoresceins; D011961:Receptors, Fc; D017455:Receptors, IgE; D011971:Receptors, Immunologic; D013388:Succinimides; D013861:Thiocyanates; D007073:Immunoglobulin E; D011092:Polyethylene Glycols; D017830:Octoxynol; C035760:bis(sulfosuccinimidyl)suberate; C011240:dithiobis(succinimidylpropionate); D016650:Fluorescein-5-isothiocyanate