Translation of type IV procollagen messenger RNA from cultured cat retinal pigment epithelial cells.
The activity of type IV procollagen mRNA was detected in the total cytoplasmic RNA prepared from normal feline retinal pigment epithelial cells in culture. The translation products contained two distinct bands in the pro alpha chain region on SDS-PAGE, which were sensitive to collagenase digestion. Corresponding bands were identified in the immunoprecipitate of the translation products after reaction with an anti-type IV collagen antiserum and separation by protein A-Sepharose column chromatography.
Shen
S
S
Stramm
L E
LE
Li
W Y
WY
Robertson
G A
GA
Aguirre
G D
GD
Rockey
J H
JH
D000818:Animals; D002415:Cats; D002478:Cells, Cultured; D003094:Collagen / Q000145:classification / Q000276:immunology; D004587:Electrophoresis, Agar Gel; D007106:Immune Sera / Q000276:immunology; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000502:physiology*; D011347:Procollagen / Q000145:classification / Q000235:genetics*; D014176:Protein Biosynthesis; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger / Q000235:genetics*
D007106:Immune Sera; D011347:Procollagen; D012333:RNA, Messenger; D012313:RNA; D003094:Collagen