Abrogation of simian virus 40 DNA-mediated transformation of primary C57BL/6 mouse embryo fibroblasts by exposure to a simian virus 40-specific cytotoxic T-lymphocyte clone.
Primary mouse embryo fibroblasts of C57BL/6 origin (B6/MEF) were transformed in vitro by transfection with simian virus 40 (SV40) DNA. The transformation frequency was markedly reduced if the SV40 DNA-transfected cultures were briefly exposed to K11 cells, an SV40-specific clone of cytotoxic T lymphocytes. This abrogation of SV40 transformation in vitro by cytotoxic T-lymphocyte clone K11 was specific, since transformation of B6/MEF cells by adenovirus type 5 DNA was not affected. The approach described here should serve as an ideal model of dissecting immunological events during in vivo tumorigenesis.
Karjalainen
H E
HE
Tevethia
M J
MJ
Tevethia
S S
SS
D000260:Adenoviruses, Human / Q000276:immunology; D000818:Animals; D000957:Antigens, Viral, Tumor / Q000276:immunology*; D002472:Cell Transformation, Viral; D002999:Clone Cells / Q000276:immunology; D004279:DNA, Viral / Q000235:genetics; D000939:Epitopes; D051379:Mice; D013539:Simian virus 40 / Q000235:genetics / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000957:Antigens, Viral, Tumor; D004279:DNA, Viral; D000939:Epitopes