Functional and phenotypic characteristics of bovine natural cytotoxic cells.
Bovine natural cytotoxic (NC) cell activity of peripheral blood leukocytes (PBL) was studied in a chromium release assay, using xenogeneic tumor cells (YAC-1, P815) and herpes virus-infected bovine fibroblasts. The activity pattern resembled that of murine natural killer cells, in that YAC-1 cells were readily lysed, whereas P815 cells were resistant. However, 10-16 h of incubation were usually required to give appreciable cell lysis. Low, but consistent NC-activity was expressed against virus-infected fibroblasts. Using various biophysical and biochemical cell-separation methods, it was found that the effector cell active against both the xenogeneic tumor cells and virus-infected fibroblast belonged to the mononuclear phagocyte system. The indications are that at least two subpopulations of the blood monocytes, differing perhaps in maturation or clonal derivation, express NC-activity.
H
H
Bielefeldt Ohmann
W C
WC
Davis
L A
LA
Babiuk
D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle / Q000276:immunology*; D002448:Cell Adhesion; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D004950:Esterases / Q000032:analysis; D007113:Immunity, Innate; D007372:Interferons / Q000096:biosynthesis; D007694:Killer Cells, Natural / Q000145:classification / Q000276:immunology*; D008223:Lymphoma / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D051379:Mice; D009000:Monocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D007372:Interferons; D004950:Esterases