Chemical factors that influence nucleocytoplasmic transport: a fluorescence photobleaching study.
The technique of fluorescence redistribution after photobleaching was used to measure the translocation rate of fluorescein-labeled dextrans across the nuclear pore complex in isolated rat liver nuclei. A transport assay system was established that could monitor the effect of biologically active molecules, e.g., ATP, GTP, cAMP on the translocation process. The results show that ATP, phosphoinositides, RNA, and insulin can enhance transport rates from 195 to 432%. It was further demonstrated that concanavalin A, but not wheat germ or soybean agglutinin, can block dextran transport completely. The effectors of dextran transport are similar to substances demonstrated to effect the efflux of RNA from isolated nuclei. A model for translocation through the nuclear pore is now presented that incorporates data from protein influx and RNA efflux experiments into a single pathway controlled by ATP.
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Jiang
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Schindler
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001693:Biological Transport, Active / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology; D003911:Dextrans / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000494:pharmacology; D037102:Lectins / Q000494:pharmacology; D008099:Liver / Q000378:metabolism; D009685:Nuclear Envelope / Q000187:drug effects / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000494:pharmacology; D010777:Photochemistry; D037121:Plant Lectins; D012313:RNA / Q000494:pharmacology; D051381:Rats; D030262:Soybean Proteins; D014909:Wheat Germ Agglutinins; D010835:Phytohemagglutinins
D000255:Adenosine Triphosphate; D003208:Concanavalin A; D000242:Cyclic AMP; D003911:Dextrans; D005456:Fluorescent Dyes; D006160:Guanosine Triphosphate; D037102:Lectins; D010716:Phosphatidylinositols; D037121:Plant Lectins; D012313:RNA; D030262:Soybean Proteins; D014909:Wheat Germ Agglutinins; C028824:soybean lectin; D010835:Phytohemagglutinins