Structural studies on O-specific polysaccharides of lipopolysaccharides from Yersinia enterocolitica serovars O:5 and O:5,27.
Lipopolysaccharides of Yersinia enterocolitica serovars O:5 and O:5,27 were shown to have a similar sugar composition, consisting of L-rhamnose, D-glucose, D-galactose, D- and L-glycero-D-manno-heptose, 2-acetamido-2-deoxy-D-glucose, 2-acetamido-2-deoxy-D-galactose, 3-deoxy-D-manno-octulosonate and D-threo-pent-2-ulose (D-xylulose). Partial hydrolysis of lipopolysaccharides with acetic acid produced rhamnans with the following repeating unit: ----3)-L-Rha rho(alpha 1----3)-L-Rha rho(alpha 1----3)-L-Rha rho(beta 1----. 13C-NMR and methylation studies of the lipopolysaccharides gave the following structure for the repeating unit of the two O-specific polysaccharides: ----3)-L-Rha rho(alpha 1----3)-L-Rha rho(alpha 1----3)-L-Rha rho(beta 1----. (formula; see text)
R P
RP
Gorshkova
E N
EN
Kalmykova
V V
VV
Isakov
Y S
YS
Ovodov
D000942:Antigens, Bacterial / Q000276:immunology*; D002241:Carbohydrates / Q000032:analysis; D006385:Hemagglutination Inhibition Tests; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D019081:O Antigens; D012703:Serotyping; D015008:Yersinia enterocolitica / Q000145:classification / Q000276:immunology*
D000942:Antigens, Bacterial; D002241:Carbohydrates; D008070:Lipopolysaccharides; D019081:O Antigens