Structural alteration of viral homologue of receptor proto-oncogene fms at carboxyl terminus.
A role for proto-oncogenes in the regulation and modulation of cell proliferation has been suggested by the findings that the B-chain of platelet-derived growth factor (PDGF) is encoded by the proto-oncogene sis and that the erb-B oncogene product is a truncated form of the epidermal growth factor (EGF) receptor. Furthermore, the product of the proto-oncogene fms (c-fms) may be related or identical to the receptor for macrophage colony-stimulating factor (CSF-1). v-fms is the transforming gene of the McDonough strain of feline sarcoma virus (SM-FeSV) and belongs to the family of src-related oncogenes which have tyrosine-specific kinase activity. Furthermore, nucleotide sequence analysis of the v-fms gene product revealed topological properties of a cell-surface receptor protein. To elucidate the features involved in the conversion of a normal cell-surface receptor gene into an oncogenic one, we have now determined the complete nucleotide sequence of a human c-fms complementary DNA. The 972-amino-acid c-fms protein has an extracellular domain, a membrane-spanning region, and a cytoplasmic tyrosine protein kinase domain. Comparison of the feline v-fms and human c-fms sequences reveals that the proteins share extensive homology but have different carboxyl termini.
L
L
Coussens
C
C
Van Beveren
D
D
Smith
E
E
Chen
R L
RL
Mitchell
C M
CM
Isacke
I M
IM
Verma
A
A
Ullrich
D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA; D066246:ErbB Receptors; D005260:Female; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D010920:Placenta / Q000032:analysis; D011061:Poly A / Q000235:genetics; D011247:Pregnancy; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D011519:Proto-Oncogenes; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D011972:Receptor, Insulin / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics*; D011973:Receptors, LDL / Q000235:genetics; D012190:Retroviridae / Q000235:genetics*; D005253:Sarcoma Viruses, Feline / Q000235:genetics*
D006023:Glycoproteins; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D011973:Receptors, LDL; D011061:Poly A; D012313:RNA; D004247:DNA; D066246:ErbB Receptors; D011972:Receptor, Insulin