Incompatibility repressor in a RepA-like replicon of the IncFI plasmid ColV2-K94.
The replication region Rep1 of the IncFI plasmid ColV2-K94 was cloned on self-replicating restriction fragments. Rep1 was structurally and functionally homologous to the RepA replicon of IncFII R plasmids. Despite this close relationship, these two replication systems were compatible with each other. The nucleotide sequence of the copA incompatibility-replication control gene of Rep1 was determined and compared with the copA sequence of RepA. Six base changes were found in a 24-base-pair span of the copA gene; these may result in the formation of a new, more stable, 49-base stem-loop structure in the potential CopA RNA repressor molecule. We postulate that these alterations weaken the interaction between RNA transcripts of the Rep1 and RepA replicons.
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PC
Weber
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Palchaudhuri
D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004265:DNA Helicases; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000235:genetics; D009690:Nucleic Acid Conformation; D010957:Plasmids; D011506:Proteins; D012329:RNA, Bacterial / Q000032:analysis; D012093:Replicon; D012097:Repressor Proteins / Q000235:genetics*; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics*
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D011506:Proteins; D012329:RNA, Bacterial; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C045264:replication initiator protein; D004262:DNA Restriction Enzymes; D004265:DNA Helicases