The differential effects produced by daunomycin and adriamycin on RNA, polynucleotides, single stranded, supercoiled DNA, and nucleosomes.
Terbium, a sensitive probe whose fluorescence is strongly enhanced when bound to unpaired guanine and xanthine bases, has been employed to study the effects of adriamycin and daunomycin on a variety of nucleotide substrates. After treatment with either drug at concentrations of less than or equal to 1:500, the fluorescence of the probe was substantially abrogated. Daunomycin, however, produced a markedly greater effect than adriamycin with rRNA, linear calf thymus DNA, and polyriboguanylic acid. The difference between the drugs was experimentally significant, suggesting that changing the C9 side group from a methyl (daunomycin) to an alcohol (adriamycin) may result in a changed base sequence specificity. The distinction was also evident when changes in electrophoretic mobility of supercoiled and nucleosomal DNA was monitored, but only at much higher (1:25) drug:DNA ratios.
Pearlman
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Simpkins
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D000818:Animals; D002417:Cattle; D004247:DNA / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D004278:DNA, Superhelical / Q000378:metabolism; D003630:Daunorubicin / Q000378:metabolism / Q000494:pharmacology*; D004317:Doxorubicin / Q000378:metabolism / Q000494:pharmacology*; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli; D006147:Guanine / Q000378:metabolism; D009707:Nucleosomes / Q000187:drug effects*; D009711:Nucleotides / Q000378:metabolism; D012329:RNA, Bacterial / Q000378:metabolism; D012335:RNA, Ribosomal / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013725:Terbium / Q000378:metabolism
D004269:DNA, Bacterial; D004278:DNA, Superhelical; D009707:Nucleosomes; D009711:Nucleotides; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D013725:Terbium; D006147:Guanine; D004317:Doxorubicin; D004247:DNA; D003630:Daunorubicin