Cell-cell interaction between rat Sertoli cells and mouse germ cells in vitro.
An antiserum against rat germ cell membranes was prepared, and after absorption with protein extracts of rat liver and kidney and mouse testis, this antiserum reacted only with rat germ cell membranes and juxtanuclear vesicles in rat Sertoli cells. Germ cell-free rat Sertoli cell monolayers were cultured in vitro. Freshly isolated mouse germ cells adhered to these monolayers within 1 h. After a minimum of 3 days of such a co-culture, immunofluorescence and immunoblotting revealed that the mouse germ cells had obtained rat-antigenic determinants in their membranes. Our results indicate that this appearance of rat-specific antigens on mouse germ cells is specific and inducible.
J A
JA
van der Donk
A L
AL
de Ruiter-Bootsma
A M
AM
Ultee-van Gessel
P J
PJ
Wauben-Penris
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002448:Cell Adhesion; D002450:Cell Communication; D002478:Cells, Cultured; D000939:Epitopes / Q000032:analysis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D011919:Rats, Inbred Strains; D012708:Sertoli Cells / Q000276:immunology / Q000502:physiology*; D013087:Spermatids / Q000276:immunology / Q000502:physiology*; D013090:Spermatocytes / Q000276:immunology / Q000502:physiology*; D013997:Time Factors
D000954:Antigens, Surface; D000939:Epitopes