Migration of host astrocytes into superior cervical sympathetic ganglia autografted into the septal nuclei or choroid fissure of adult rats.
Adult astrocytes and their processes, identified by glial fibrillary acidic protein immunohistochemistry and by electron microscopy, migrate into superior cervical ganglia auto-transplanted into the choroid fissure or septal nuclei of adult rats. Migration routes were along the blood vessels, and along the Schwann cell bundles of the transplant. Ultrastructurally, astrocytic processes could be seen to lie in direct contact with Schwann cell processes within the basal lamina enwrapping the Schwann cell and its associated axons. Around the region of the host/transplant interface, the astrocytes were transformed into flattened cells with many short, irregular, fringe-like processes, but within the depths of the transplant mass they resumed a more stellate configuration. Glial fibrillary acidic protein immunoreactivity was present within the intrinsic satellite and Schwann cells of the grafted ganglia, but at a much lower level than in the host astrocytes. It is concluded that reactive astrocytes from adult host central nervous system migrate into peripheral ganglionic transplants, where they differentiate and establish organized arrangements with the ganglionic elements.
C F
CF
Zhou
J M
JM
Lawrence
R J
RJ
Morris
G
G
Raisman
D000818:Animals; D001253:Astrocytes / Q000502:physiology*; D002465:Cell Movement; D005260:Female; D005728:Ganglia, Sympathetic / Q000502:physiology / Q000637:transplantation*; D006085:Graft Survival; D008854:Microscopy, Electron; D009474:Neurons / Q000502:physiology / Q000637:transplantation* / Q000648:ultrastructure; D011184:Postoperative Period; D051381:Rats; D011919:Rats, Inbred Strains; D012686:Septal Nuclei / Q000502:physiology* / Q000648:ultrastructure; D013194:Staining and Labeling; D014182:Transplantation, Autologous