DNA demethylation induced by 5-azacytidine does not affect fragile X expression.
Experiments were performed to determine the role of DNA demethylation in fragile X expression. Fragile X positive lymphoblastoid cells were treated with 5-azacytidine and harvested for analysis of fragile X expression both directly following treatment and after a recovery period in the absence of the drug. The effectiveness of 5-azacytidine treatment in inducing DNA demethylation was concurrently monitored by analysis of methylation changes at random autosomal loci in isolated DNA from treated cells. Under conditions where 5-azacytidine was found to inhibit fragile X expression, no DNA demethylation was observed. At the time when demethylation did occur, fragile X expression was not affected. These results strongly indicate that DNA demethylation is not involved in fragile X expression.
Glover
T W
TW
Coyle-Morris
J
J
Pearce-Birge
L
L
Berger
C
C
Gemmill
R M
RM
D001374:Azacitidine / Q000494:pharmacology*; D002460:Cell Line; D002873:Chromosome Fragility; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D005600:Fragile X Syndrome / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D005819:Genetic Markers; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008745:Methylation; D014960:X Chromosome / Q000187:drug effects / Q000378:metabolism
D005819:Genetic Markers; D004247:DNA; D004262:DNA Restriction Enzymes; D001374:Azacitidine