[Experimental research following intratumor bleomycin use in the nude mouse model of oral mucosa cancer and the clinical pilot study].
Experiments concerning the kinetics and bio-distribution of 57Co-Bleomycin in three different lines of the squamous cell carcinoma of the oral cavity in a nude-mouse model yielded a more than tenfold higher concentration after a single intratumoral (i.t.) application of an aqueous solution of 57Co-labelled Bleomycin in spite of a remarkable radioactivity-wash in the tumor up to 48 hours post applicationem, compared to the intravenous (i.v.) injection. For the investigation of the radiopharmacon within the tumor we used the macro-autoradiographic method. The xenografts have been removed and cut (5 micrograms) 1;5; 24, and 48 hours after the Bleomycin administration, respectively. These preparations have been covered with an autoradiographic film and exposed for about three weeks. After this an inhomogenous distribution of the radioactive nuclide was produced within the tumor, and a particularly high activity-concentration could be demonstrated in the necrotic tumor areas. The results obtained from the animal experiments have been corroborated by a pilot-study consisting of i.t. Bleomycin application in nine patients with a carcinoma of the oral mucosa.
Fröhlich
M
M
Henke
E
E
Arnold
W
W
Naundorf
H
H
Gens
J
J
D000818:Animals; D001761:Bleomycin / Q000008:administration & dosage / Q000378:metabolism / Q000627:therapeutic use*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000378:metabolism; D004195:Disease Models, Animal; D004341:Drug Evaluation; D004353:Drug Evaluation, Preclinical; D006801:Humans; D007700:Kinetics; D008207:Lymphatic Metastasis; D051379:Mice; D008819:Mice, Nude; D009062:Mouth Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D009368:Neoplasm Transplantation; D011300:Preoperative Care; D013997:Time Factors; D014062:Tongue Neoplasms / Q000188:drug therapy / Q000378:metabolism
D001761:Bleomycin
Experimentelle Untersuchungen nach intratumoraler Bleomycin-Applikation am nude-Maus-Modell des Mundschleimhautkarzinoms und klinische Pilotstudie.