The protective effect of the 4-5S immune RNA against Trypanosoma cruzi infection in mice.
The immune RNA (iRNA) was extracted from the spleens of mice infected with Trypanosoma cruzi. The 4-5S iRNA, obtained by sucrose gradient centrifugation, was effective in reducing the parasitemia and mortality of mice challenged with a virulent strain of T. cruzi. No such activity was found in the RNA fraction obtained from the spleens of normal (4-5S nRNA) animals. Mouse peritoneal macrophages exposed to the 4-5S iRNA were able to inhibit the growth of T. cruzi. These results suggest that the protective effect of the 4-5S iRNA against T. cruzi infection could be due to its ability in enhancing macrophage resistance to this parasite.
M C
MC
Bertolini
F L
FL
De Lucca
D000818:Animals; D014355:Chagas Disease / Q000276:immunology* / Q000469:parasitology; D008264:Macrophages / Q000276:immunology / Q000469:parasitology; D008297:Male; D051379:Mice; D012313:RNA / Q000276:immunology*; D014349:Trypanosoma cruzi / Q000254:growth & development
C013674:RNA, immune; D012313:RNA