Meal-stimulated absorption of water and electrolytes in canine jejunum.
After a meal, the absorption of water and electrolytes from the jejunal lumen increases. This meal-induced jejunal absorption occurs in jejunal segments out of normal gastrointestinal continuity. The experimental model used 25-cm proximal jejunal Thiry-Vella loops in awake dogs (n = 72 observations) to evaluate the mechanisms involved in meal-induced jejunal absorption, seeking to define the source or sources of the proabsorptive signal. Specifically, we evaluated the jejunal absorptive response to a standard meal, a standard meal plus cholinergic blockage using atropine, a sham-fed meal, a gavage-fed meal, and gastric distension with balloon and gavage water. Both the standard meal and the gavage-fed meal induced a prompt, sustained, and significant (P less than 0.0001) increase in the absorption of H2O, Na+, and Cl-. Atropine significantly reduced the magnitude of the postmeal absorptive response (P less than 0.05) compared with the standard meal alone. The sham-fed meal, gastric balloon distension, and gavage water did not alter jejunal absorption. Vagal nerve integrity after cervical esophageal manipulation was verified by gastric acid output and gastrin response to stimuli. These data support a role for cholinergic modulation of meal-stimulated jejunal absorption via a cephalic-phase-independent and gastric-distension-independent mechanism.
Yeo
C J
CJ
Department of Surgery, Johns Hopkins Medical Institutions, Baltimore, Maryland 21205.
Bastidas
J A
JA
Schmieg
R E
RE
Zinner
M J
MJ
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D004285:Dogs; D004435:Eating; D004573:Electrolytes / Q000378:metabolism*; D004947:Esophagus / Q000502:physiology; D005260:Female; D005744:Gastric Acid / Q000378:metabolism; D005755:Gastrins / Q000097:blood; D007408:Intestinal Absorption / Q000187:drug effects; D007583:Jejunum / Q000502:physiology*; D009130:Muscle, Smooth / Q000502:physiology; D013270:Stomach / Q000502:physiology*; D007507:Therapeutic Irrigation; D013997:Time Factors; D014867:Water
D004573:Electrolytes; D005755:Gastrins; D014867:Water; D001285:Atropine