Interaction of monoclonal antibodies to electroplaque acetylcholine receptors with the alpha-bungarotoxin binding site of goldfish brain.
Polyclonal and monoclonal antibodies raised against acetylcholine receptors from Torpedo californica and Electrophorus electricus electroplaque were tested for interaction with the [125I]alpha-bungarotoxin binding protein of goldfish brain. A subset of monoclonal antibodies which recognize the main immunogenic region of the alpha subunit of the Electrophorus acetylcholine receptor interacted at high affinity with the [125I]alpha-bungarotoxin binding protein. Using immunofluorescence, these antibodies were shown to label the same layers of the optic tectum as [125I]alpha-bungarotoxin.
Henley
J M
JM
Mynlieff
M
M
Lindstrom
J M
JM
Oswald
R E
RE
D000818:Animals; D000911:Antibodies, Monoclonal; D003429:Cross Reactions; D004557:Electric Organ / Q000276:immunology*; D004593:Electrophorus; D000939:Epitopes / Q000032:analysis; D006054:Goldfish; D011950:Receptors, Cholinergic / Q000276:immunology*; D011978:Receptors, Nicotinic; D013477:Superior Colliculi / Q000276:immunology*; D014101:Torpedo; D064569:alpha7 Nicotinic Acetylcholine Receptor
D000911:Antibodies, Monoclonal; D000939:Epitopes; D011950:Receptors, Cholinergic; D011978:Receptors, Nicotinic; D064569:alpha7 Nicotinic Acetylcholine Receptor