Identification of a surface epitope of human erythropoietin with anti-peptide antibodies.
Antibodies reactive with human erythropoietin were isolated from the serum of rabbits immunized with a twenty-six amino acid synthetic polypeptide corresponding to a proposed NH2-terminal sequence of the hormone. As shown by inhibition with peptide fragments, those antibodies that bound to erythropoietin recognized the (8-15) domain, strongly suggesting tht this region is exposed on the hormone's surface. This was confirmed by affinity purification of these antibodies on immobilized fragment (8-15). These results provide insight into the tertiary structure of human erythropoietin and suggest uses for the sequence-specific antibodies in labeling the hormone.
A J
AJ
Sytkowski
D M
DM
Wojchowski
D000595:Amino Acid Sequence; D001665:Binding Sites; D000939:Epitopes / Q000032:analysis*; D004921:Erythropoietin / Q000032:analysis / Q000276:immunology*; D006801:Humans; D007700:Kinetics; D010446:Peptide Fragments / Q000276:immunology*; D011487:Protein Conformation
D000939:Epitopes; D010446:Peptide Fragments; D004921:Erythropoietin