Maintenance of desmosomes in mouse hepatocytes after drug-induced rearrangement of cytokeratin filament material. Demonstration of independence of desmosomes and intermediate-sized filaments.
The distribution of desmosomes and cytokeratin filaments (tonofilaments) in hepatocytes of normal mice and those intoxicated with griseofulvin was studied by immunofluorescence microscopy. Treatment with griseofulvin over prolonged periods of time resulted in the dissociation of cytokeratin filaments from the plasma membrane and the inclusions of cytokeratin material in typical cytoplasmic aggregates, i.e. "Mallory bodies". However, such hepatocytes still displayed typical desmosomal arrays, including rather regularly spaced desmosomes along the bile canaliculi. These observations show that, in this tissue, desmosomes are able to maintain their characteristic positions along the plasma membrane after disconnection of the intermediate filament cytoskeleton. This indicates that maintenance of desmosomal integrity and position is independent of desmosome anchorage to tonofilaments. The results are discussed in relation to current concepts of desmosome formation and turnover.
H
H
Denk
E
E
Lackinger
P
P
Cowin
W W
WW
Franke
D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000032:analysis; D003598:Cytoskeletal Proteins; D003599:Cytoskeleton / Q000648:ultrastructure*; D051180:Desmoplakins; D003896:Desmosomes / Q000032:analysis / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D006118:Griseofulvin / Q000494:pharmacology*; D007382:Intermediate Filaments / Q000032:analysis / Q000187:drug effects / Q000648:ultrastructure*; D007633:Keratins / Q000032:analysis*; D008099:Liver / Q000032:analysis / Q000187:drug effects / Q000648:ultrastructure*; D008297:Male; D008565:Membrane Proteins / Q000032:analysis; D051379:Mice; D008856:Microscopy, Fluorescence
D003598:Cytoskeletal Proteins; D051180:Desmoplakins; D008565:Membrane Proteins; D006118:Griseofulvin; D007633:Keratins