Monoclonal antibodies distinguish between the head portions of two myosin isozymes from chicken breast muscle.
Monoclonal antibodies against chicken breast myosin and its subfragment-1(S-1) were produced. One antibody, 2G41, reacted with S-1 containing a light chain 3 (LC3), but not with another S-1 containing a light chain 1 (LC1) or a mixture of the light chains. A structural difference can be assumed to exist between the head portions of the two myosin isozymes. Antigenicity of S-1 toward 2G41 could not be detected after tryptic digestion into three fragments of 50K, 27K, and 20K daltons. Another monoclonal antibody, M68, was obtained from mice immunized with myosin. M68 preferably recognized the heavy chain from S-1 containing LC3 rather than that from that containing LC1 or S-1. M68 reacted with the 27K fragment among the three.
N
N
Kikuchi
M
M
Shibata
M
M
Yoshida
K
K
Yagi
I
I
Azuma
M
M
Naiki
S
S
Kubo
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification*; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D008321:Mammary Glands, Animal; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D009132:Muscles / Q000201:enzymology*; D009218:Myosins / Q000276:immunology*; D011809:Quinones / Q000096:biosynthesis* / Q000378:metabolism; D014357:Trypsin / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D011809:Quinones; D014357:Trypsin; D009218:Myosins