Multiple labeling of cellular constituents by combining surface reflection interference and fluorescence microscopy.
In this paper the technique for visualizing cytoskeleton in detergent-extracted cultured cells by surface reflection interference (SRI) microscopy after staining with the protein dye Coomassie Brilliant Blue (SRI-CooB technique) is used in conjunction with fluorescently-labeled antibodies or with other fluorescent probes to detect a number of constituents in the same cultured cell. Because SRI-CooB technique preferentially visualizes microfilament bundles along the ventral aspect of cells adhering to a glass substratum, we feel that this simple and rapid technique has great potential in studies of cell-substratum adhesiveness and of adhesion-related cytoskeletal organization.
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D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D002645:Chickens; D003584:Cytological Techniques; D003599:Cytoskeleton / Q000032:analysis*; D005353:Fibronectins / Q000032:analysis; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D006168:Guinea Pigs; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods; D008857:Microscopy, Interference / Q000379:methods; D012394:Rosaniline Dyes; D013194:Staining and Labeling
D005353:Fibronectins; D005456:Fluorescent Dyes; D012394:Rosaniline Dyes; C004692:coomassie Brilliant Blue