Vinculin and 36 kDa protein are not tyrosine-phosphorylated in Rous sarcoma virus infected cells which have been treated with interferon.
The expression of membrane-associated transformation-specific parameters was analyzed in de novo Rous sarcoma virus (strain SR-RSV-D) infected chicken embryo fibroblasts pretreated with homologous interferon. Cellular morphology, hexose transport, microfilament organization, and tyrosine-phosphate content of two primary substrates of the transformation-generating viral kinase, pp60src, were found indistinguishable from non-infected controls. These observations support the hypothesis that vinculin and possibly 36 kDa protein are involved in microfilament organization and that tyrosine-phosphorylation of these structural proteins is a prerequisite for the rearrangement of microfilaments during transformation. In de novo infection, interferon pretreatment reduces viral protein synthesis and pp60src activity as compared to non-treated, SR-RSV-D infected cells. However, the phosphotyrosine content of total cellular proteins as measured under steady state conditions is as high in interferon-pretreated as in nontreated transformed cells.
Strube
W
W
Jungwirth
C
C
Ziemiecki
A
A
Jockusch
B M
BM
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000201:enzymology; D002472:Cell Transformation, Viral / Q000187:drug effects; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D007372:Interferons / Q000494:pharmacology*; D008970:Molecular Weight; D009124:Muscle Proteins / Q000378:metabolism*; D015688:Oncogene Protein pp60(v-src); D010766:Phosphorylation; D012191:Retroviridae Proteins / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism*; D016596:Vinculin; D014764:Viral Proteins / Q000378:metabolism*
D009124:Muscle Proteins; D012191:Retroviridae Proteins; D014764:Viral Proteins; D016596:Vinculin; D014443:Tyrosine; D007372:Interferons; D015688:Oncogene Protein pp60(v-src)