Molecular detrapping and band narrowing with high frequency modulation of pulsed field electrophoresis.
In high electric fields, megabase DNA fragments are found to be trapped, i.e. to enter or migrate in the gel only very slowly, if at all, leading to very broad electrophoretic bands and loss of separation. As a consequence, low electric fields are usually used to separate these molecules by pulsed field electrophoretic methods. We report here that high-frequency pulses eliminate the molecular trapping found in continuous fields. When high frequency pulses are used to modulate the longer pulses used in pulsed field electrophoresis, narrower bands result, and higher fields can be used. We suggest that this is due to effects that occur on the length scale of a single pore.
Turmel
C
C
Xerox Research Centre of Canada, Mississauga, Ontario.
Brassard
E
E
Slater
G W
GW
Noolandi
J
J
D002175:Candida / Q000235:genetics; D004271:DNA, Fungal / Q000302:isolation & purification*; D004560:Electricity; D004586:Electrophoresis / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics
D004271:DNA, Fungal