Covalent change of major intrinsic polypeptide (MIP26K) of lens membrane during human senile cataractogenesis.
Polyclonal antisera have been made against synthetic peptides corresponding to the C-terminal octapeptide and N-terminal nonapeptide of bovine MIP26K. Western blot analysis demonstrated significant binding of the C-terminal antiserum to MIP26K of both normal and cataractous human lens. In contrast, the N-terminal antiserum bound to MIP26K of normal lenses, but failed to bind to MIP26K of 7 out of 10 cataractous lenses studied. These results demonstrate for the first time, a covalent change in MIP26K during human cataractogenesis, and strongly suggest that the location of this change is in the N-terminal region of the polypeptide.
Takemoto
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Takehana
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D000368:Aged; D000595:Amino Acid Sequence; D020346:Aquaporins; D002386:Cataract / Q000378:metabolism*; D000939:Epitopes; D005136:Eye Proteins / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007163:Immunosorbent Techniques; D007365:Intercellular Junctions / Q000276:immunology / Q000378:metabolism*; D007908:Lens, Crystalline / Q000276:immunology / Q000378:metabolism*; D008562:Membrane Glycoproteins
D020346:Aquaporins; D000939:Epitopes; D005136:Eye Proteins; D008562:Membrane Glycoproteins; C030328:aquaporin 0