Dust from carpeted and smooth floors--III. Trials on denaturation of allergenic proteins by household cleaning solutions and chemical detergents.
The effects of detergents on antigens and allergens of birch and timothy grass pollen extracts, codfish, hen egg-white, cat dander and house dust mite were investigated by crossed immunoelectrophoresis (CIE), crossed radio-immunoelectrophoresis (CRIE), radio-allergosorbent test (RAST) inhibition and quantitative precipitation test using laser nephelometry. Nine household cleaning solutions and five chemical detergents were tested. Higher concentrations than recommended for regular cleaning purposes were used as no influence on the antigenic and allergenic activities could be detected using the usual concentrations. Soft soap, guanidine hydrochloride and sodium lauryl sulphate induced most modifications of the antigens and allergens tested. None of the detergents totally destroyed the antigenic and allergenic activities of the selected material, even when used in concentrations up to 10 times that recommended. The materials used for carpet cleaning would not be able to impose any denaturing effects on allergens left on carpets after cleaning.
Allergy Research Group, Laboratory of Clinical Biochemistry, University Hospital, Bergen, Norway.
T
T
Dybendal
H
H
Vik
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S
Elsayed
D000485:Allergens; D003902:Detergents / Q000494:pharmacology*; D004391:Dust; D005160:Facility Design and Construction; D005430:Floors and Floorcoverings; D011489:Protein Denaturation / Q000187:drug effects*; D013501:Surface-Active Agents / Q000494:pharmacology*
D000485:Allergens; D003902:Detergents; D004391:Dust; D013501:Surface-Active Agents