The role of T cell accessory molecules in the generation of class II-specific xenogeneic cytolytic T cells.
In the generation of allogeneic, hapten-modified and virus-specific cytotoxic T cell (CTL) responses there is usually a requirement for T-T interaction between the T helper cell (TH) and the precursor CTL (CTL). We have investigated the role of a TH signal in the induction of a xenogeneic mouse antihuman CTL response by using membranes and liposomes bearing the xenogeneic antigen to stimulate primed responders. The TH signal can be achieved by either an Ia-restricted, L3T4+ DR-specific T cell or by the addition of nonspecific T helper factors(s). This signal is delivered to an Lyt-2+, L3T4-DR-specific CTL to generate active xenogeneic (xeno-) CTL. The roles of the T cell accessory molecules L3T4, Lyt-2, and LFA-1 in the generation of and target cell lysis by xeno-CTL are investigated.
J L
JL
Greenstein
J A
JA
Foran
J C
JC
Gorga
S J
SJ
Burakoff
D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte; D000950:Antigens, Ly / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D002450:Cell Communication; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D016169:Lymphocyte Function-Associated Antigen-1; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D051381:Rats; D011917:Rats, Inbred Lew; D013045:Species Specificity; D013602:T-Lymphocytes, Cytotoxic / Q000145:classification / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology* / Q000378:metabolism
D000945:Antigens, Differentiation, T-Lymphocyte; D000950:Antigens, Ly; D000954:Antigens, Surface; D000939:Epitopes; D000949:Histocompatibility Antigens Class II; D016169:Lymphocyte Function-Associated Antigen-1