Presence of endothelin-1-like immunoreactivity in human cerebrospinal fluid.
By use of radioimmunoassay (RIA) specific for endothelin-1 (ET-1), we have studied whether ET-1-like immunoreactivity (LI) exists in human cerebrospinal fluid (CSF). Human CSF from patients with old cerebrovascular disease contained far greater amounts of ET-1-LI (28 +/- 1.3 pg/ml, n = 9) than normal human plasma. Reverse-phase HPLC coupled with ET-1 RIA revealed a single major ET-1-LI component in human CSF extract coeluting with standard ET-1. These data suggest ET-1 is a neuropeptide secreted from neural tissues in humans.
Hirata
Y
Y
Department of Medicine, Tokyo Medical and Dental University, Japan.
Matsunaga
T
T
Ando
K
K
Furukawa
T
T
Tsukagoshi
H
H
Marumo
F
F
D000368:Aged; D002543:Cerebral Hemorrhage / Q000134:cerebrospinal fluid; D002544:Cerebral Infarction / Q000134:cerebrospinal fluid; D002851:Chromatography, High Pressure Liquid; D016232:Endothelins; D004730:Endothelium, Vascular; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000134:cerebrospinal fluid*; D011863:Radioimmunoassay
D016232:Endothelins; D010455:Peptides