Histamine-producing cell stimulating factor (HCSF) and interleukin 3 (IL 3): evidence for two distinct molecular entities.
Because homogeneously purified interleukin 3 (IL 3) can induce an increase in histamine synthesis by normal bone marrow cells like histamine-producing cell stimulating Factor (HCSF), it has been suggested that HCSF and IL 3 might be identical. In this paper, we provide evidence that HCSF activity can be obtained without IL 3 activity (determined by the proliferation of an IL 3-dependent cell line). This distinction between HCSF and IL 3 has been achieved in three different ways: a) biochemical separation of HCSF from IL 3 in crude secondary MLC supernatants with description of different physicochemical characteristics of the two molecules; b) demonstration of an HCSF activity devoid of IL 3 activity in P388D1 conditioned medium, with the same characteristics as HCSF produced during secondary MLC but different from WEHI-3-derived HCSF activity resulting from IL 3; c) demonstration of a lack of inhibition of HCSF-induced histamine synthesis by anti-IL 3 immunoglobulins that clearly diminish IL 3-induced histamine production. These results demonstrate that two distinct factors (IL 3 and HCSF) promote histamine synthesis, but that IL 3 has more general effects on hemopoietic cells, whereas HCSF seems to be restricted to histamine-producing cells.
Dy
M
M
Lebel
B
B
Schneider
E
E
D000818:Animals; D001853:Bone Marrow / Q000378:metabolism; D001854:Bone Marrow Cells; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003470:Culture Media; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D006636:Histamine Release; D007136:Immunoglobulins / Q000502:physiology; D007377:Interleukin-3; D007941:Leukemia P388 / Q000378:metabolism; D007951:Leukemia, Myeloid / Q000378:metabolism; D007959:Lymphocyte Culture Test, Mixed; D008222:Lymphokines / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D008407:Mast Cells / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA
D003470:Culture Media; D007136:Immunoglobulins; D007377:Interleukin-3; D008222:Lymphokines