Ultrastructural localization of rRNA in HeLa cells, rat liver cells and Xenopus laevis oocytes by means of the monoclonal antibody--protein a--gold technique.
The postembedding localization of rRNA was investigated in ultrathin sections of HeLa cells, rat liver and Xenopus laevis oocytes by means of the monoclonal antibody to rRNA and protein A-gold technique. The incidence of gold particles was highest in nucleoli and cytoplasmic areas containing ribosomes. The chromosomes were labelled less than the surrounding cytoplasm in mitotic HeLa cells. In nucleoli of HeLa cells and rat hepatocytes, the labelling of areas containing ribonucleoprotein components was greater than the labelling of fibrillar centres. In segregated nucleoli of X. laevis oocytes, the labelling of the granular region substantially exceeded that of the fibrillar regions. The incidence of nucleoplasmic gold particles in interphasic HeLa cells was found to be slightly increased in the vicinity of nucleoli. The labelling of clusters of interchromatin granules in rat hepatocytes was not significantly different from that of the rest of the nucleophasmic interchromatin spaces.
I
I
Raska
P
P
Valouch
B L
BL
Armbruster
M
M
Hinterberger
A
A
Malý
J
J
Vorlícek
K
K
Smetana
E
E
Kellenberger
D000818:Animals; D000911:Antibodies, Monoclonal; D006046:Gold; D006367:HeLa Cells / Q000032:analysis; D006651:Histocytochemistry; D006801:Humans; D008099:Liver / Q000032:analysis* / Q000166:cytology; D008854:Microscopy, Electron; D009865:Oocytes / Q000032:analysis* / Q000166:cytology; D012335:RNA, Ribosomal / Q000032:analysis*; D051381:Rats; D013194:Staining and Labeling; D013205:Staphylococcal Protein A; D014982:Xenopus laevis
D000911:Antibodies, Monoclonal; D012335:RNA, Ribosomal; D013205:Staphylococcal Protein A; D006046:Gold