[Use of automatic cytophotometric analysis for assessing the hepatotropic action of ethanol].
It has been shown in experimental chronic intoxication that there is a close correlation between the dose of ethanol, microscopic appearance of the liver, the content of DNA, RNA, protein, glycogen in hepatocytes and structural parameters of nuclear chromatin of these cells. It is concluded that automatic cytophotometric analysis of the structure of hepatocyte nuclei holds promise for an accurate quantitative evaluation of the hepatotropic action of chemical substances and drugs.
Zhukotskiĭ
A V
AV
Shchegolev
A I
AI
Iavorskiĭ
A N
AN
Kogan
E M
EM
D000437:Alcoholism / Q000378:metabolism; D000818:Animals; D003584:Cytological Techniques; D004247:DNA / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000633:toxicity*; D005470:Fluorometry / Q000379:methods; D006651:Histocytochemistry; D006801:Humans; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008112:Liver Glycogen / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D051381:Rats
D008112:Liver Glycogen; D011506:Proteins; D000431:Ethanol; D012313:RNA; D004247:DNA
Primenenie avtomaticheskogo tsitofotometricheskogo analiza dlia otsenki gepatotropnogo deĭstviia étanola.