[Inhibition of fading in FITC-labelled coverslip preparations].
The influence of anti-fading-substances on the kinetic of fading FITC-labelled coverslip cultures was tested and a quantitative valuation theses compounds was performed. The half-life fluorescence intensity was detected with 2 different methods. The effect of 21 chemical substances was investigated whereof 7 compounds was determined as effective fading-inhibitors. The velocity constants of the 1st order (kapp) of photochemical reaction increase with rising hydrogen activity.
Senkpiel
K
K
Klagge
E
E
Körting
H J
HJ
D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins / Q000378:metabolism; D005456:Fluorescent Dyes; D006207:Half-Life; D008856:Microscopy, Fluorescence; D013194:Staining and Labeling / Q000379:methods*; D013861:Thiocyanates / Q000378:metabolism
D005452:Fluoresceins; D005456:Fluorescent Dyes; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate
Untersuchungen zur Inhibition des Ausbleichens von FITC-markierten Deckglaspräparaten.