Mast cell and neutrophil interactions: a role for superoxide anion and histamine.
Histamine inhibits superoxide anion (O-2) production from human neutrophils stimulated by N-formylmethionyl-leucyl-phenylalanine (FMLP). The effects of histamine are dose-dependent and competitively antagonized by cimetidine. When passively sensitized rat serosal mast cells and human neutrophils are mixed together, O-2 production from FMLP-activated granulocytes is significantly reduced, following mast cell degranulation by acetylcholine. These inhibitory effects can be counteracted by cimetidine. Exposure of non-sensitized rat mast cells to FMLP-stimulated human neutrophils causes histamine release. These results suggest bidirectional control mechanisms between mast cells and neutrophils, that further stress the role of histamine in regulating inflammatory processes.
R
R
Fantozzi
S
S
Brunelleschi
L
L
Giuliattini
P
P
Blandina
E
E
Masini
G
G
Cavallo
P F
PF
Mannaioni
D000818:Animals; D002927:Cimetidine / Q000494:pharmacology; D006632:Histamine / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013481:Superoxides / Q000378:metabolism*
D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D002927:Cimetidine; D006632:Histamine