Inhibitory action of phorbol myristate acetate on histamine secretion and polyphosphoinositide turnover induced by compound 48/80 in mast cells.
Rat peritoneal mast cells which had been preincubated with phorbol myristate acetate (PMA, 10 - 100 ng/ml) for 5 min did not elicit the full histamine secretion induced by a potent secretagogue, compound 48/80. Furthermore, this PMA-treatment was found to inhibit the agonist-mediated hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) in [32P]labeled cells. However, it was also observed that the level of [32P]PIP2 was markedly reduced by 5 min-incubation with PMA. This suggests the enhanced hydrolysis of PIP2 by PMA which was reflected in a greater formation of inositol trisphosphate (IP3). These observations indicate that the formation of IP3 may not be profoundly related to secretory response in mast cells.
Y
Y
Okano
H
H
Takagi
S
S
Nakashima
T
T
Tohmatsu
Y
Y
Nozawa
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000818:Animals; D002118:Calcium / Q000378:metabolism; D006636:Histamine Release / Q000187:drug effects*; D007294:Inositol / Q000378:metabolism; D007546:Isoquinolines / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D010704:Phorbols / Q000494:pharmacology*; D010712:Phosphatidic Acids / Q000378:metabolism; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols / Q000378:metabolism*; D010879:Piperazines / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D007546:Isoquinolines; D010704:Phorbols; D010712:Phosphatidic Acids; D018129:Phosphatidylinositol Phosphates; D010716:Phosphatidylinositols; D010879:Piperazines; C037178:phosphatidylinositol 4-phosphate; D003189:p-Methoxy-N-methylphenethylamine; D007294:Inositol; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium