Changes in hepatic RNA, poly(A)+RNA, and poly(A)-RNA during the acute phase response to inflammation.
The steady state changes in total rat hepatic cytoplasmic RNA, poly(A)+ RNA and poly(A)-RNA were assessed in response to turpentine induced inflammation. From 18 to 24 h after injury, cytoplasmic RNA doubled, while poly(A)+ RNA peaked at 24 h, 3.5 times over control animals. Cell-free translation showed significant increases in messenger RNA levels beginning at 18 h. Gel electrophoresis of translation products revealed significant increases in several polypeptides and a decrease in others. Poly(A)-RNA from control and injured rats translated to an insignificant level and the electrophoretic gel patterns of their proteins were similar. Furthermore, no change had occurred in the 3' poly(A)-sequences during the course of inflammation.
Demczuk
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Chandler
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D000208:Acute Disease; D000818:Animals; D002474:Cell-Free System; D007249:Inflammation / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D011061:Poly A / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger; D051381:Rats; D011919:Rats, Inbred Strains
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA