Combined immunoreaction and Papanicolaou's stain on cytological smears.
The method described below combines an immunoreaction with Papanicolaou's stain on cytological smears. For the immunoreaction, the avidin-biotin-complex (ABC) method was used. The method was tested on various cytological material with the monoclonal antibody lu-5 and two polyclonal antibodies (anti-keratin and anti-chymotrypsin). Wet fixation of the smears with a modified Delaunay's solution is recommended. Drying of the material impairs immunoreactivity. The main advantages of the technique are the clear-cut permanent immunostaining and the preservation of the nuclear structure, permitting a combined immuncytological characterization of cellular products and conventional cyto-diagnosis.
P
P
Dalquen
D
D
Bittel
F
F
Gudat
J
J
von Overbeck
P U
PU
Heitz
D000911:Antibodies, Monoclonal; D001360:Avidin; D001710:Biotin; D002918:Chymotrypsin / Q000276:immunology; D003581:Cytodiagnosis; D003584:Cytological Techniques; D004848:Epithelium / Q000473:pathology; D006651:Histocytochemistry; D006801:Humans; D007158:Immunologic Techniques; D007633:Keratins / Q000276:immunology; D009369:Neoplasms / Q000473:pathology*; D010996:Pleural Effusion / Q000473:pathology; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D001360:Avidin; D007633:Keratins; D001710:Biotin; D002918:Chymotrypsin