Epidermal growth factor (EGF) stimulates EGF receptor synthesis.
Epidermal growth factor (EGF) binds to the extracellular domain of a specific 170,000-dalton transmembrane glycoprotein; this results in rapid removal of both ligand and receptor from the cell surface. In WB cells, a rat hepatic epithelial cell line, ligand-directed receptor internalization leads to receptor degradation. We tested whether the EGF receptor was replenished at a constitutive or enhanced rate following EGF binding by immunoprecipitating biosynthetically labeled EGF receptor from cells cultured with [35S]methionine. EGF stimulated receptor synthesis within 2 h in a dose-dependent manner; this was particularly evident when examining the nascent form of the receptor. To determine the site of EGF action, total WB cell RNA was transferred to nitrocellulose paper after electrophoresis and was hybridized to cDNA probes from both the external and cytoplasmic coding regions of the human EGF receptor. EGF increased receptor mRNA by 3-5-fold. Therefore, at least in some cells, the surface action of EGF that leads to EGF receptor degradation is counterbalanced by a positive effect on receptor synthesis.
Earp
H S
HS
Austin
K S
KS
Blaisdell
J
J
Rubin
R A
RA
Nelson
K G
KG
Lee
L W
LW
Grisham
J W
JW
D000818:Animals; D002460:Cell Line; D004247:DNA / Q000235:genetics; D004815:Epidermal Growth Factor / Q000378:metabolism / Q000494:pharmacology*; D004848:Epithelium / Q000378:metabolism; D066246:ErbB Receptors; D007163:Immunosorbent Techniques; D008099:Liver / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000235:genetics
D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D004815:Epidermal Growth Factor; D012313:RNA; D004247:DNA; D008715:Methionine; D066246:ErbB Receptors