Conditions affecting the differentiation of F9 teratocarcinoma cells: potentiation of response by cyclic AMP.
F9 cells maintained in culture were shown to have a reduced ability to differentiate. The cells produced decreased amounts of alphafetoprotein when induced with retinoic acid. We show that consistent responses can be recovered after passage of F9 cells as a tumor. In addition, optimal differentiation of F9 cells to visceral endoderm may be achieved by the addition of very low concentrations of dibutyryl cyclic AMP (dbcAMP) to the medium.
A
A
Grover
E D
ED
Adamson
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003470:Culture Media; D054278:Embryonal Carcinoma Stem Cells; D004707:Endoderm / Q000166:cytology; D007797:Laminin / Q000096:biosynthesis; D051379:Mice; D014411:Neoplastic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013724:Teratoma / Q000473:pathology; D014212:Tretinoin / Q000494:pharmacology; D000509:alpha-Fetoproteins / Q000096:biosynthesis
D003470:Culture Media; D007797:Laminin; D000509:alpha-Fetoproteins; D014212:Tretinoin; D003994:Bucladesine