Inhibition of human T-cell lymphotropic virus type III in vitro by phosphonoformate.
Phosphonoformate, an inhibitor of reverse transcriptase in a number of retroviruses, was shown to have a dose-related inhibitory effect on human T-cell lymphotropic virus type III (HTLV-III) replication in the H9 cell line in vitro. HTLV-III replication was eliminated at a concentration of 680 mumol, a non-cytotoxic dose. A lower dose of 132 mumol inhibited HTLV-III replication by more than 98%, as measured by reverse transcriptase activity, compared with untreated infected cultures. Reverse transcriptase activity in HTLV-III particles was completely inhibited by 5.0 mumol phosphonoformate.
Sandstrom
E G
EG
Kaplan
J C
JC
Byington
R E
RE
Hirsch
M S
MS
D002460:Cell Line; D017977:Deltaretrovirus / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D004305:Dose-Response Relationship, Drug; D017245:Foscarnet; D006801:Humans; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D010746:Phosphonoacetic Acid / Q000031:analogs & derivatives / Q000494:pharmacology*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013997:Time Factors; D014779:Virus Replication / Q000187:drug effects*
D009943:Organophosphorus Compounds; D017245:Foscarnet; D012194:RNA-Directed DNA Polymerase; D010746:Phosphonoacetic Acid