Evaluation of a monoclonal antibody to the K99 fimbrial adhesin produced by Escherichia coli enterotoxigenic for calves, lambs and piglets.
All the K99+ Escherichia coli grown at 37 degrees C stained strongly with a peroxidase labelled K99 monoclonal antibody using a direct immunoperoxidase staining procedure. There was no reaction when these bacteria were cultured at 18 degrees C or when K99- E coli were grown at either temperature. The binding of the monoclonal antibody to K99 antigen was inhibited by OK antisera to heterologous K99+ E coli but OK antisera to E coli producing adhesins other than K99 were without effect. Using the slide agglutination test the reactions of the monoclonal antibody were identical to those of a polyclonal antiserum to K99 when both were used in parallel to examine 100 K99+ E coli from at least 10 somatic O groups and 1308 K99+ E coli from at least 82 different somatic O groups submitted for routine serological typing in England or the, USA. The monoclonal antibody reacted with K99+ E coli in cryostat sections of the ileum from a piglet infected with E coli strain B44 (O9: K30, K99, F41) but there was no reaction with similar material from piglets infected by E coli strains 1751 (O101: F41), X177/81 (O9: K103, 987P) or Abbotstown (O149: K91, K88ac).
J A
JA
Morris
C J
CJ
Thorns
C
C
Boarer
R A
RA
Wilson
D018830:Adhesins, Escherichia coli; D000372:Agglutination Tests / Q000662:veterinary; D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D001427:Bacterial Toxins; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D003967:Diarrhea / Q000175:diagnosis / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D004927:Escherichia coli Infections / Q000175:diagnosis / Q000662:veterinary*; D007124:Immunoenzyme Techniques; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*
D018830:Adhesins, Escherichia coli; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D000939:Epitopes; C031429:K99 antigen