Phorbol ester enhances deoxynucleoside incorporation while inhibiting proliferation of K-562 cells.
Recently a new technique has been developed to study bromodeoxyuridine incorporation into DNA as a function of the cell cycle phase distribution. We have made the observation that treatment of the hematopoietic cell line K-562 results in a complete and immediate cessation of cell growth, although tritiated thymidine incorporation is markedly elevated for as long as 5 days. We have made use of that newly described technique to study this phenomenon.
Colamonici
O R
OR
Trepel
J B
JB
Neckers
L M
LM
D001973:Bromodeoxyuridine; D002453:Cell Cycle / Q000187:drug effects*; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis*; D003853:Deoxyribonucleosides / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D007399:Interphase; D010704:Phorbols / Q000494:pharmacology*; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D003853:Deoxyribonucleosides; D010704:Phorbols; D012313:RNA; D004247:DNA; D001973:Bromodeoxyuridine; D013755:Tetradecanoylphorbol Acetate