Influence of D-galactosamine on the kinetics of metabolic processes for two intermediate metabolites, 9-hydroxybenzo(a)pyrene and 3-hydroxybenzo(a)pyrene, in 3T3 and RTG2 cells.
PAH metabolism is known to proceed in two successive steps, the first step resulting in the production of activated metabolites which are subsequently transformed by the different pathways involved in the second step. Microspectrofluorometry enables the study of the kinetics of these steps in living intact cells into which no imbalance has been artificially introduced. We used this technique to check the influence of pre-incubation with D-galactosamine on the kinetics of the detoxification step. 9- and 3-hydroxybenzo(a)pyrene (OH-B(a)P) were selected as fluorescent substrates because they are potential substrates for the different pathways of the second step. The physiological cell status was controlled at the level of the intrinsic cellular fluorescence. Pre-incubation with D-galactosamine results in a strong decrease of the experimental rate constants characteristic of the metabolism of 9- and 3-OH-B(a)P in both RTG2 and 3T3 cells. Moreover, such pre-incubation leads to a strong decrease of the transitory intracellular accumulation of 3-O-glucuronide when 3-OH-B(a)P is used as substrate for 3T3 cells. Nevertheless, it cannot be said that both phenols cannot be used as substrates by MFOs and STase, at least in rigorous experimental conditions.
Lautier
D
D
URA CNRS 1289 Quantitative Microfluorometry and Cellular Pharmacokinetics, University of Perpignan, France.
Anthelme
B
B
Salmon
J M
JM
Vigo
J
J
Viallet
P
P
D000818:Animals; D001580:Benzopyrenes / Q000378:metabolism*; D002460:Cell Line; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D005688:Galactosamine / Q000502:physiology*; D006651:Histocytochemistry / Q000379:methods; D051379:Mice; D013050:Spectrometry, Fluorescence
D001580:Benzopyrenes; C021337:9-hydroxybenzo(a)pyrene; C021338:3-hydroxybenzo(a)pyrene; D005688:Galactosamine