Isolation of P2 protein--reactive T-cell lines from human blood.
Human T-cell lines reactive with the peripheral nerve myelin protein, P2 protein, were isolated from the peripheral blood of 4 normal persons and 1 patient with Guillain-Barré syndrome. These predominantly helper phenotype T-cell lines were isolated and maintained in vitro by antigen stimulation followed by culture with interleukin 2. Myelin basic protein-reactive T cells were also isolated in parallel from the same subjects as antigen specificity controls. T cells recognizing myelin basic protein did not respond to P2 protein, nor did P2-reactive cells respond to myelin basic protein. These findings suggest that a potential for autoimmune reactivity with peripheral nervous system myelin antigens may exist for both normal persons and some patients with neurological disease.
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J
Burns
L J
LJ
Krasner
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A
Rostami
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D
Pleasure
D000328:Adult; D001773:Blood Cells / Q000276:immunology*; D002460:Cell Line; D046508:Culture Techniques / Q000379:methods; D000939:Epitopes; D006801:Humans; D008297:Male; D004676:Myelin Basic Protein / Q000276:immunology*; D018996:Myelin P2 Protein; D009444:Neuritis, Autoimmune, Experimental / Q000139:chemically induced; D010641:Phenotype; D011129:Polyradiculoneuropathy / Q000097:blood; D013601:T-Lymphocytes / Q000276:immunology*
D000939:Epitopes; D004676:Myelin Basic Protein; D018996:Myelin P2 Protein