MHC class II gene products specific autoreactive T cell clones provide inducer as well as amplifier function for B cell Ig production.
Autoreactive T lymphocytes were generated by culturing human peripheral blood mononuclear cells with an antigen-specific/MHC restricted autologous inducer T cell, termed RW17C and subsequently cloned in soft agar. The majority of such clones expressed the T3+T4+T8-T11+Ia+ phenotype and were directed at autologous class II MHC gene products found on B cells, macrophages and B lymphoblastoid cells as judged by their proliferative response to the latter. For this recognition, the clones employed a T3-Ti molecular complex and a T4 structure analogous to those found on allospecific T cells. Perhaps more importantly, it was observed that the same AC (autoreactive clone) induced autologous B cells to produce high levels of immunoglobulin in the absence of exogenous antigen and could synergize with the RW17C clone to effect maximal B cell Ig production. In addition, supernatant from T3-Ti triggering of AC clone induced both polyclonal proliferation and differentiation of small B lymphocytes. These results support the notion that such autoreactive cells can function in a physiologic amplifier role by facilitating induction via an internal set of signals (i.e. autologous MHC).
Bensussan
A
A
Milanese
C
C
Meuer
S C
SC
Reinherz
E L
EL
D000911:Antibodies, Monoclonal; D000917:Antibody Formation; D000938:Antigen-Presenting Cells / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology*; D000939:Epitopes; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006801:Humans; D007376:Interleukin-2 / Q000276:immunology; D008213:Lymphocyte Activation; D008565:Membrane Proteins / Q000276:immunology; D008970:Molecular Weight; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D000939:Epitopes; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D008565:Membrane Proteins; D011948:Receptors, Antigen, T-Cell