[Fluorophotometry as a method for the detection of permeability changes in the blood and aqueous humor barrier].
The increase in permeability of the blood-aqueous barrier after Nd-YAG laser iridotomy and the protective effect of locally applied indomethacin were investigated in an experimental animal study using a computerized fluorophotometer. The permeability of the blood-aqueous barrier was evaluated by three different molecules: fluorescein-sodium (molecular weight 330), fluorescein-labeled dextran 70 000 (molecular weight 70 000) and fluorescein-labeled dextran 150 000 (molecular weight 150 000). The fluorescein concentration of the anterior chamber was measured in 36 eyes (18 rabbits) as well as in a control group of 9 eyes (9 rabbits) at fixed times after intravenous dye injection. Fluorescein leakage occurred, but no leakage of fluorescein-labeled dextrans was visible in the control group. Laser iridotomy induced a time-dependent increase in anterior segment permeability to fluorescein and fluorescein-labeled dextrans. Disturbance of the blood-aqueous barrier was effectively reduced by topical pretreatment with indomethacin, which mainly inhibited the leakage of the fluorescein-labeled dextrans. The clinical relevance of the laser-induced breakdown of the blood-aqueous barrier and the protective effect of a prophylactic pharmacotherapy are discussed.
W
W
Schrems
P
P
Grosskopf
D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism*; D002199:Capillary Permeability / Q000187:drug effects; D003911:Dextrans / Q000378:metabolism; D019793:Fluorescein; D005452:Fluoresceins / Q000378:metabolism*; D007213:Indomethacin / Q000494:pharmacology; D007498:Iris / Q000601:surgery*; D053685:Laser Therapy; D011183:Postoperative Complications / Q000097:blood*; D011817:Rabbits
D003911:Dextrans; D005452:Fluoresceins; D019793:Fluorescein; D007213:Indomethacin
Fluorophotometrie als Methode zum Nachweis von Permeabilitätsänderungen der Blut-Kammerwasser-Schranke.