Synthetic peptide assays to detect human immunodeficiency virus types 1 and 2 in seropositive individuals.
Detection of antibodies to human immunodeficiency virus type 1 by enzyme-linked immunosorbent assay is the standard method to screen blood products. With the description of human immunodeficiency virus type 2 as a second retrovirus involved in the pathogenesis of acquired immunodeficiency syndrome, additional laboratory testing is required to screen blood products. However, owing to the serological cross-reactivity of human immunodeficiency virus types 1 and 2 at the molecular level, it is impossible to distinguish human immunodeficiency virus type 2-infected blood with enzyme-linked immunosorbent assays formatted with human immunodeficiency virus type 1 viral lysates. Synthetic peptides from the conserved envelope region of the human immunodeficiency virus transmembrane protein offer a new approach to develop site-specific diagnostic assays that will identify and discriminate between human immunodeficiency virus types 1 and 2.
Smith
R S
RS
Johnson & Johnson Biotechnology Center, San Diego, CA 92121.
Parks
E
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D000595:Amino Acid Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D015686:Gene Products, env / Q000276:immunology; D015658:HIV Infections / Q000175:diagnosis*; D006679:HIV Seropositivity / Q000175:diagnosis; D015497:HIV-1 / Q000276:immunology / Q000302:isolation & purification*; D015498:HIV-2 / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007118:Immunoassay; D008969:Molecular Sequence Data
D015686:Gene Products, env