The effects of lipid composition on the rate and extent of heme binding to membranes.
The effects of membrane composition on heme binding to large unilamellar vesicles were examined using 30 separate phospholipid mixtures. Although there was some variation, most lecithins with Tm values less than or equal to 20 degrees C showed overall equilibrium partition constants equal to approximately 5 x 10(5) and association and dissociation partition rate constants equal to approximately 3 x 10(6) s-1 and 7 s-1, respectively, for CO-heme binding at 30 degrees C. A sharp decrease in the association rate for CO-heme uptake was observed as the lipid vesicles changed from liquid-crystalline to the gel phase. The addition of dicetyl phosphate or dimyristoylphosphatidylglycerol, which are negatively charged at neutral pH, decreased the affinity of the vesicles for CO-heme. The association rate and equilibrium partition constants for CO-heme uptake in unsaturated lecithins were unaffected by cholesterol content at levels up to 40%/mol. The affinity of saturated dimyristoylphosphatidylcholine (DMPC) vesicles for CO-heme decreased with increasing cholesterol content at 30 degrees C. This effect appears to be related to the influence of cholesterol on the DMPC phase transition temperature (Tm) since at low temperatures (less than or equal to 20 degrees C) little CO-heme binds to vesicles composed of DMPC even in the absence of cholesterol.
Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77251.
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Olson
D002248:Carbon Monoxide; D002784:Cholesterol; D004134:Dimyristoylphosphatidylcholine; D006418:Heme / Q000378:metabolism*; D007700:Kinetics; D008081:Liposomes; D008433:Mathematics; D008954:Models, Biological; D010755:Organophosphates; D010713:Phosphatidylcholines; D013329:Structure-Activity Relationship; D013816:Thermodynamics
D008081:Liposomes; D010755:Organophosphates; D010713:Phosphatidylcholines; C002290:dicetylphosphate; D006418:Heme; D002248:Carbon Monoxide; D002784:Cholesterol; D004134:Dimyristoylphosphatidylcholine