Heterogeneity of p15(E)-related polypeptides expressed by MuLV-infected cells.
The p15(E)-related polypeptides of radiation leukemia virus (RadLV)-derived viruses and of cells infected with prototype MuLV were analyzed by immunoprecipitation, SDS-PAGE, and immunofluorescence analyses. It was found that the p15(E)-related molecules of ecotropic and xenotropic viruses derived from RadLV lymphoma cell lines were distinguishable by reactivity with monoclonal anti-p15(E) antibodies and by SDS-PAGE profile. Ecotropic MuLV of RadLV origin encoded the p15(E)a antigen and produced a Pr15(E) of 20K MW. In contrast, xenotropic virus derived from RadLV did not express the p15(E)a antigen and by SDS-PAGE its Pr15(E) migrated at 21K. A previously undescribed, p15(E)-related molecule of 18.5K MW was associated with xenotropic RadLV. These differences were also reproduced by the prototype ecotropic, xenotropic, and dualtropic viruses.
Bach
R G
RG
Meruelo
D
D
D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D009052:Leukemia Virus, Murine / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D008223:Lymphoma; D051379:Mice; D008907:Mink; D008970:Molecular Weight; D010641:Phenotype; D014759:Viral Envelope Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D000939:Epitopes; D014759:Viral Envelope Proteins