Interactions in red blood cells between fatty acids and either snake venom cardiotoxin or halothane.
Phospholipase A2 (PLA2) activity enhances snake venom cardiotoxin (CTX)-induced and general anesthetic (halothane)-induced hemolysis of red blood cells. In the case of halothane-induced hemolysis, this effect appears to be related primarily to free fatty acids. In the present study, the interaction between CTXs and halothane and the effects of different free fatty acids on cardiotoxin and halothane-induced hemolysis were examined. The hemolytic actions of halothane and a CTX from Naja naja kaouthia venom were examined in erythrocytes with different phospholipid and free fatty acid composition from five species. The extent of hemolysis by CTX or halothane was dependent upon the species examined and appeared to be inversely related to the amount of saturated free fatty acid in the membrane. The order of susceptibility of red blood cells from five species to hemolysis was similar for halothane- and N. n. kaouthia CTX-induced hemolysis, but very different for osmotic fragility. The slope of the hemolysis dose-response curve was considerably steeper for halothane than for CTX. Hemolysis due to N. n. kaouthia CTX was greatly increased by halothane in erythrocytes from humans and horses and to a lesser extent in erythrocytes from sheep, goats and cows. Hemolysis induced by halothane and the N. n. kaouthia or Naja naja atra CTXs was enhanced by unsaturated fatty acids. In contrast, hemolysis induced by halothane was decreased and that caused by the two CTXs was relatively unaffected by saturated fatty acids. Halothane and CTXs differ in their exact mechanisms, but appear to act upon similar fatty acid-sensitive processes.
Fletcher
J E
JE
Department of Anesthesiology, Hahnemann University, Philadelphia, PA 19102-1192.
Jiang
M S
MS
Tripolitis
L
L
Smith
L A
LA
Beech
J
J
D000818:Animals; D002784:Cholesterol / Q000097:blood; D004179:Cobra Cardiotoxin Proteins / Q000494:pharmacology*; D004546:Elapid Venoms / Q000494:pharmacology*; D004912:Erythrocytes / Q000032:analysis / Q000187:drug effects*; D005230:Fatty Acids, Nonesterified / Q000097:blood* / Q000494:pharmacology; D006221:Halothane / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D010743:Phospholipids / Q000097:blood; D012910:Snake Venoms / Q000494:pharmacology*
D004179:Cobra Cardiotoxin Proteins; D004546:Elapid Venoms; D005230:Fatty Acids, Nonesterified; D010743:Phospholipids; D012910:Snake Venoms; D002784:Cholesterol; D006221:Halothane