Expression of the glycoprotein gene from a fish rhabdovirus by using baculovirus vectors.
A cDNA fragment containing the gene encoding the glycoprotein of infectious hematopoietic necrosis virus was inserted into Autographa californica baculovirus vectors under the control of the polyhedrin promoter. A 66-kilodalton protein, identical in size to the glycosylated glycoprotein of infectious hematopoietic necrosis virus, was expressed at high levels in Spodoptera frugiperda cells infected with the recombinant viruses. The expressed protein reacted with antiserum to the glycoprotein on Western blots (immunoblots).
Department of Microbiology, Oregon State University, Corvallis 97331-3804.
J F
JF
Koener
J A
JA
Leong
D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D005399:Fishes / Q000382:microbiology*; D015870:Gene Expression; D005814:Genes, Viral; D006023:Glycoproteins / Q000235:genetics* / Q000302:isolation & purification; D007304:Insect Viruses / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D015183:Restriction Mapping; D012209:Rhabdoviridae / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics*
D006023:Glycoproteins; D015678:Viral Structural Proteins