Fine-granular cationic iron colloid. Its preparation, physicochemical characteristics and histochemical use for the detection of ionized anionic groups.
In order to obtain distinct and reliable information concerning the localization of ionized anionic groups in tissues, fine-granular cationic ferric hydroxide colloid solution (Fe-Cac-f) was newly devised. This can be obtained by boiling a mixture of ferric chloride and ammonium cacodylate solutions. The colloid particles of Fe-Cac-f are about 1.0 nm in size, i.e., one-fifth of the size of ferric cacodylate colloid (Fe-Cac; Seno et al. 1983a). As with Fe-Cac, Fe-Cac-f particles in the pH range of 1.6-7.6 carry a positive electric charge, but the latter show a better permeation of tissues. Using the Prussian blue reaction, Fe-Cac-f gives a distinct deep-blue color and can be used for the detection of anionic groups of acid mucopolysaccharides and proteins by light microscopy. It is also useful for detecting the exact sites of ionized anionic groups in deep tissue areas using electron microscopy.
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D000818:Animals; D001152:Arsenicals; D002101:Cacodylic Acid; D002356:Cartilage / Q000032:analysis; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002712:Chlorides; D002809:Chondroitin Sulfates; D003102:Colloids; D004347:Drug Interactions; D005290:Ferric Compounds / Q000138:chemical synthesis*; D006025:Glycosaminoglycans / Q000032:analysis; D007475:Ion Exchange Resins; D007501:Iron / Q000138:chemical synthesis*; D007678:Kidney Glomerulus / Q000032:analysis; D008297:Male; D011134:Polysaccharides; D011145:Polyvinyls; D051381:Rats; D011919:Rats, Inbred Strains; D012710:Serum Albumin, Bovine; D013194:Staining and Labeling / Q000379:methods*
D001152:Arsenicals; D002712:Chlorides; D003102:Colloids; D005290:Ferric Compounds; D006025:Glycosaminoglycans; D007475:Ion Exchange Resins; D011134:Polysaccharides; D011145:Polyvinyls; C019050:polyvinyl sulfate; D012710:Serum Albumin, Bovine; D002809:Chondroitin Sulfates; C014420:colominic acid; D002101:Cacodylic Acid; D007501:Iron; C024555:ferric chloride