Effect of cytosine arabinoside on differentiation of normal human bone marrow cells.
Human normal bone marrow cells were evaluated for alteration of differentiation after exposure for seven days to 10(-12)-10(-9) M cytosine arabinoside (ARA-C) in liquid culture. An increased number of induced cells had the morphologic appearance of mature monocytes-macrophages; they adhered to petri dishes, reacted positively to fluoride-sensitive naphthyl acetate esterase, and specifically bound My4 monoclonal antibody (MCAb). Assessment of phagocytosis and killing of Candida albicans (CA) by cultured monocytes-macrophages exposed to ARA-C demonstrated that treated cells had the same capacity to phagocytose and kill CA as did untreated cells. In semisolid culture, low doses of ARA-C did not affect myeloid colony growth. These studies indicate that ARA-C enhances monocytic differentiation of normal human bone marrow cells in liquid culture.
A
A
Nagler
Y
Y
Kletter
I
I
Ricklis
E
E
Gazit
I
I
Tatarsky
I
I
Fabian
D001853:Bone Marrow / Q000187:drug effects* / Q000276:immunology; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003561:Cytarabine / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D006801:Humans; D008264:Macrophages / Q000187:drug effects; D009000:Monocytes / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects; D013194:Staining and Labeling
D003561:Cytarabine