Axonal transport of beta-receptors during the response to axonal injury and repair in locus coeruleus neurons.
Injections of the catecholamine neurotoxin, 6-hydroxydopamine, were placed in the ascending locus coeruleus (LC) pathway in the right cerebral cortex of rats partially destroying the noradrenergic projection to the somatosensory cortex. Norepinephrine (NE) levels fell to a nadir of 49% of control over the first 14 days, associated with a 40% increase in the number of beta-adrenoreceptor binding sites (labeled with [3H]dihydroalprenolol; [3H]DHA) in the denervated cortex. Both NE levels and cortical beta-receptor binding returned to control levels by 28 days. Similar changes, of lesser magnitude, also occurred in the unlesioned, left somatosensory cortex. Catecholamine histofluorescence studies supported these findings of denervation and reinnervation of the right cortex over a 3-month period. Anterograde axonal transport of beta-receptors was assessed by measuring the accumulation of beta-receptor binding sites ([3H]DHA) behind a second lesion placed in the more proximal portion of the ipsilateral LC pathway. Anterograde transport was completely blocked at 4 days, during the initial fall of NE levels, then was increased to 200% of control at 14-21 days, when recovery of cortical NE levels was beginning, and then returned towards control levels by 2-3 months when normal NE levels had been restored.(ABSTRACT TRUNCATED AT 250 WORDS)
B E
BE
Levin
W P
WP
Battisti
M
M
Murray
D000818:Animals; D001370:Axonal Transport; D001927:Brain Diseases / Q000139:chemically induced / Q000503:physiopathology; D002395:Catecholamines / Q000378:metabolism; D006892:Hydroxydopamines; D008125:Locus Coeruleus; D008297:Male; D009434:Neural Pathways; D009473:Neuronal Plasticity; D016627:Oxidopamine; D051381:Rats; D011919:Rats, Inbred Strains; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D013003:Somatosensory Cortex
D002395:Catecholamines; D006892:Hydroxydopamines; D011943:Receptors, Adrenergic, beta; D016627:Oxidopamine