A rapid method for purification of myelin basic protein.
A rapid procedure for purification of myelin basic protein has been developed. White matter is delipidated with 2-butanol, and the residue is extracted at pH 7.5 and 8.5. Myelin basic protein is solubilized by extraction in acetate buffer, pH 4.5. The entire procedure requires less than 4 h, and gives homogeneous protein essentially free of protease activity. This procedure can be scaled down to process milligram amounts of white matter; thus it can be very useful for purification of myelin basic protein from very limited amounts of human white matter obtained during surgery.
Bellini
T
T
Rippa
M
M
Matteuzzi
M
M
Dallocchio
F
F
D000085:Acetates; D019342:Acetic Acid; D000818:Animals; D001923:Brain Chemistry; D002021:Buffers; D000440:Butanols; D002417:Cattle; D002845:Chromatography; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D004676:Myelin Basic Protein / Q000302:isolation & purification*; D010447:Peptide Hydrolases / Q000302:isolation & purification
D000085:Acetates; D002021:Buffers; D000440:Butanols; D004676:Myelin Basic Protein; C043958:2-butanol; D010447:Peptide Hydrolases; D019342:Acetic Acid