Recognition of the alpha-1 and alpha-2 domains of H-2 molecules by allospecific cloned T cells.
Previously we had shown that allospecific bulk cultures of cytolytic T lymphocytes lysed the products of cloned class I major histocompatibility genes expressed after DNA-mediated gene transfer. In these experiments, performed by using cloned allospecific T cell effectors, a T cell hybridoma, and recombinant DNA technology, we have been able to map determinants recognized by these T cell clones to the alpha-1 domain of H-2Dd and the alpha-2 domain of H-2Ld (four of eight clones). Target cells used were L cells (H-2k), expressing wild type or hybrid H-2 antigens of H-2d origin. Thus, for the first time determinants recognized by cloned T cells are found in the recombined alpha-1 and alpha-2 domains.
Reiss
C S
CS
Greenstein
J L
JL
Crimmins
M A
MA
Liu
L L
LL
Rao
A
A
Maziaraz
R T
RT
Murre
C
C
Burakoff
S J
SJ
D000595:Amino Acid Sequence; D000818:Animals; D002999:Clone Cells; D000939:Epitopes; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology*; D051379:Mice; D011110:Polymorphism, Genetic; D013601:T-Lymphocytes / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D000939:Epitopes; D006183:H-2 Antigens