Detection of a subgenomic mRNA for gene V, the putative reverse transcriptase gene of cauliflower mosaic virus.
Polypeptides synthesized in vitro in rabbit reticulocyte lysates, directed by poly(A)+ RNA isolated from turnip leaves infected with cauliflower mosaic virus (CaMV), were analysed by polyacrylamide gel electrophoresis. Following translation of virus-specific RNA purified by hybrid-selection using CaMV DNA immobilized on DBM papers, the polypeptides observed included the viral gene VI inclusion body protein P62, and a larger product, P75, together with several smaller polypeptides. By translating RNA hybrid-selected with restriction fragments encompassing the CaMV genome, a mRNA for P75 has been mapped to gene V. These results, together with sucrose gradient ultracentrifugation studies, suggest that a CaMV gene V mRNA is a sub-genomic transcript of approximately 2.5Kb and 22S. The expression strategy of the CaMV genome is discussed in the light of our findings.
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Plant
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Covey
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Grierson
D002874:Chromosome Mapping; D005796:Genes; D005814:Genes, Viral; D008970:Molecular Weight; D009029:Mosaic Viruses / Q000235:genetics*; D011061:Poly A / Q000235:genetics; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D012194:RNA-Directed DNA Polymerase / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics
D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D011061:Poly A; D012313:RNA; D012194:RNA-Directed DNA Polymerase