Adjuvant actions of polyclonal lymphocyte activators. V. Proliferation of macrophage colony-forming cells in the draining lymph node.
We investigated the action of various polyclonal lymphocyte activators (PLA) on the proliferation of macrophage colony-forming cells in vivo at the local site. As PLA, Klebsiella pneumoniae 03 lipopolysaccharide (K03 LPS), Escherichia coli 0111 lipopolysaccharide (E. coli LPS), dextran sulfate (DS), concanavalin A (Con A), phytohemaggulutinin (PHA), polyadenylic-polyuridylic acid (poly(A:U], polyinosinic-polycytidylic acid (poly(I:C], and pokeweed mitogen (PWM) were used. All PLA tested acted to proliferate macrophage colony-forming cells in the draining lymph node at a late stage after subcutaneous injection. The order of strength of this action of PLA was K03 LPS greater than E. coli LPS greater than Con A greater than DS greater than PHA, PWM, poly(I:C), and poly(A:U), which corresponded to the order of strength of their adjuvant action in initiating helper-T-cell response to subcutaneous injection of aggregate-free bovine gamma-globulin. The detailed relationship between the proliferation of macrophage colony-forming cells and the adjuvant action of PLA is discussed.
Yokochi
T
T
Nakashima
I
I
Kato
N
N
Miyadai
T
T
Yoshida
K
K
Kimura
Y
Y
D000276:Adjuvants, Immunologic; D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D005260:Female; D007279:Injections, Subcutaneous; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008198:Lymph Nodes / Q000166:cytology; D008264:Macrophages / Q000166:cytology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000166:cytology; D000511:alpha-Macroglobulins / Q000008:administration & dosage / Q000494:pharmacology*
D000276:Adjuvants, Immunologic; D008070:Lipopolysaccharides; D000511:alpha-Macroglobulins; C032306:polyclonal B cell activator