Lectin inhibition system for determination of concanavalin A glycoprotein complexes dissociation constants in agarose affinity electrophoresis.
A new system for lectin-glycoprotein complexes dissociation constants (K) determination is presented. The system is based on agarose affinity electrophoresis where equal lectin (Con A) concentrations are inhibited by variable specific sugar (alpha-methyl-mannoside) amounts. Moreover, the system allows lectin-sugar inhibition constants (Ki) studies. For determination of mechanisms as well as K and Ki values calculations mathematical equations are developed. Values of K for two variants of alpha 1 acid-glycoprotein, two variants of alpha 1-antitrypsin, one variant of alphafetoprotein and Ki for Con A-alpha-MM are calculated according to the introduced equations and compared in a computed system. Moreover, the influence of sugar on lectin-glycoproteins interaction is demonstrated and discussed.
Mackiewicz
A
A
Wiktorowicz
K
K
Mackiewicz
S
S
D001172:Arthritis, Rheumatoid / Q000097:blood; D003208:Concanavalin A / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D037102:Lectins; D046911:Macromolecular Substances; D009961:Orosomucoid / Q000378:metabolism; D011485:Protein Binding; D000515:alpha 1-Antitrypsin / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000378:metabolism
D006023:Glycoproteins; D037102:Lectins; D046911:Macromolecular Substances; D009961:Orosomucoid; D000515:alpha 1-Antitrypsin; D000509:alpha-Fetoproteins; D003208:Concanavalin A