Analysis of sugars in glycoproteins by high-pressure liquid chromatography.
A method for analyzing the carbohydrate composition of glycoproteins and similar glycoconjugates by methanolysis followed by reverse-phase high-pressure liquid chromatography of the perbenzoylated methyl glycosides has been developed. As described, the method is capable of quantifying sugars in the 1- to 10-nmol range while further optimization of procedures may increase the usable sensitivity by a factor of 10 or greater. Improved yields of the sugar derivatives have been achieved by incorporating several modifications of the original methanolysis procedure. This, together with the use of high-pressure liquid chromatography rather than gas chromatography for separating the sugar derivatives, eliminates the need for empirically determined molar response ratios.
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D000818:Animals; D002241:Carbohydrates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D006023:Glycoproteins / Q000032:analysis*; D006027:Glycosides / Q000032:analysis; D006868:Hydrolysis; D007785:Lactose / Q000031:analogs & derivatives / Q000032:analysis; D009077:Mucins / Q000032:analysis; D009844:Oligosaccharides / Q000032:analysis; D012794:Sialic Acids / Q000032:analysis; D013552:Swine; D000509:alpha-Fetoproteins / Q000032:analysis
D002241:Carbohydrates; D006023:Glycoproteins; D006027:Glycosides; D009077:Mucins; D009844:Oligosaccharides; D012794:Sialic Acids; D000509:alpha-Fetoproteins; D007785:Lactose