Retrograde axonal transport of asialoglycoproteins in mouse trigeminal neurons in vivo and in rat dorsal root ganglia neurons in vitro.
Different terminal sugars of the glycoprotein orosomucoid were exposed by sequential glycosidase digestions. The orosomucoid and its different derivatives were conjugated to horseradish peroxidase by a two-step glutaraldehyde coupling procedure, injected into the snout of 12-day-old mice or exposed to dorsal root ganglia neurons from embryonic rats, cultivated in a two chamber system. A marked increase in transport of the conjugates in the trigeminal and dorsal root ganglia neurons was observed histochemically after removal of sialic acid, exposing galactose as the terminal sugar. Quantitative hydrolysis of galactose residues resulted in reduced uptake. The data suggest the presence of a galactose-recognition molecule in the axon-terminal membrane, involved in retrograde axonal transport.
M
M
Gustafsson
G
G
Andersson
A
A
Hjerpe
K
K
Kristensson
D000818:Animals; D001212:Asialoglycoproteins; D001370:Axonal Transport; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002621:Chemistry; D004705:Endocytosis; D005727:Ganglia, Spinal / Q000378:metabolism*; D006651:Histocytochemistry; D051379:Mice; D009961:Orosomucoid / Q000031:analogs & derivatives / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012668:Trigeminal Ganglion / Q000378:metabolism*; D014276:Trigeminal Nerve / Q000378:metabolism*
D001212:Asialoglycoproteins; D009961:Orosomucoid; C017030:asialoorosomucoid