Characterization of matteuccin, the 2.2S storage protein of the ostrich fern. Evolutionary relationship to angiosperm seed storage proteins.
The 2.2S spore storage protein (matteuccin) of the ostrich fern, Matteuccia struthiopteris, has been isolated and characterized. It is a small basic protein consisting of two disulfide-linked polypeptides with approximate molecular masses of 3.0 kDa and 8.0 kDa. At least four different isoforms exist where two of the forms differ from the other by having a slightly smaller heavy chain. Amino acid analysis reveals that the 2.2S protein is rich in arginine. Almost complete amino acid sequence information was obtained for the light chain and a partial sequence for the heavy chain. Amino acid sequence comparison reveals that this protein shows a high similarity to seed storage proteins in different angiosperm species in spite of the fact that the common ancestor of ferns and angiosperms lived more than 300 million years ago.
Rödin
J
J
Department of Cell Research, Swedish University of Agricultural Sciences, Uppsala Biomedical Center.
Rask
L
L
D055516:2S Albumins, Plant; D000595:Amino Acid Sequence; D005075:Biological Evolution; D003429:Cross Reactions; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000276:immunology / Q000302:isolation & purification; D010940:Plant Proteins / Q000276:immunology / Q000302:isolation & purification; D010944:Plants / Q000032:analysis; D011233:Precipitin Tests; D012639:Seeds / Q000032:analysis
D055516:2S Albumins, Plant; D010455:Peptides; D010940:Plant Proteins; C065802:matteuccin protein, Matteuccia struthiopteris; C050363:napin protein, Brassica napus