Inhibition by sakyomicin A of avian myeloblastosis virus reverse transcriptase and proliferation of AIDS-associated virus (HTLV-III/LAV).
In the course of screening for inhibitors of reverse transcriptase, we have isolated an inhibitor from a strain of Nocardia and identified it as sakyomicin A. The antibiotic blocks avian myeloblastosis virus reverse transcriptase reaction: IC50 was ca. 30 micrograms/ml by the method employed. The drug affects proliferation of HTLV-III/LAV in HTLV-I-carrying MT-4 cells: ca. 60% inhibition was observed at an antibiotic concentration of 1.0 microgram/ml and ca. 20% inhibition at 0.1 microgram/ml, and there was no significant cytotoxicity.
Tanaka
N
N
Okabe
T
T
Tanaka
N
N
Take
Y
Y
Inouye
Y
Y
Nakamura
S
S
Nakashima
H
H
Yamamoto
N
N
D001354:Avian Leukosis Virus / Q000201:enzymology*; D009189:Avian Myeloblastosis Virus / Q000201:enzymology*; D016227:Benzoquinones; D002460:Cell Line; D017977:Deltaretrovirus / Q000254:growth & development*; D004353:Drug Evaluation, Preclinical; D006801:Humans; D011809:Quinones / Q000494:pharmacology; D018894:Reverse Transcriptase Inhibitors; D014779:Virus Replication / Q000187:drug effects*
D016227:Benzoquinones; D011809:Quinones; D018894:Reverse Transcriptase Inhibitors; C042536:sakyomicin A