Hepatocellular carcinoma after thorotrast exposure: establishment of a new cell line (Mz-Hep-1).
A human hepatoma cell line, associated with thorotrast exposure, from an hepatitis B marker-negative patient was established as a permanent cell line (Mz-Hep-1) in tissue culture. Histology of the primary tumor, as well as phase contrast, transmission and scanning electron microscopy of the cultured cells showed typical characteristics of liver cells. Mz-Hep-1 cells secreted complement components (C2, C3, C4), carcinoembryonic antigen, lactate dehydrogenase, chymotrypsin, haptoglobin and retinol-binding protein and expressed HLA-, transferrin-, blood group B-related determinants and complement component C5 and carcinoembryonic antigen on their cell surface. Mz-Hep-1 cells represent the first human hepatoma cell line, which is strongly associated with a carcinogen.
W G
WG
Dippold
H P
HP
Dienes
A
A
Knuth
W
W
Sachsse
W
W
Prellwitz
D
D
Bitter-Suermann
K H
KH
Meyer zum Büschenfelde
D000792:Angiography / Q000009:adverse effects; D002272:Carcinoembryonic Antigen / Q000032:analysis; D006528:Carcinoma, Hepatocellular / Q000209:etiology* / Q000276:immunology / Q000473:pathology / Q000648:ultrastructure; D002453:Cell Cycle; D002460:Cell Line; D003165:Complement System Proteins / Q000276:immunology; D005260:Female; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006513:Hepatitis B e Antigens / Q000032:analysis; D006801:Humans; D007621:Karyotyping; D008113:Liver Neoplasms / Q000209:etiology* / Q000276:immunology / Q000473:pathology / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D013911:Thorium Dioxide / Q000009:adverse effects*; D000509:alpha-Fetoproteins / Q000032:analysis
D002272:Carcinoembryonic Antigen; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D000509:alpha-Fetoproteins; D003165:Complement System Proteins; D013911:Thorium Dioxide