Protective immunity induced in mice by detoxified salmonella lipopolysaccharide.
C3H/HeNMTV mice were immunised intraperitoneally (i.p.) with lipopolysaccharide (LPS) or detoxified LPS (D-LPS) derived from Salmonella typhimurium strain SR-11. In both cases, effective protection was achieved against a challenge dose of greater than 2 x 10(2) LD50 of the same organism given by i.p. injection. However, by comparison with LPS, approximately 6- to 10-fold more of D-LPS by weight was needed to protect mice to an equivalent degree. Histopathological studies showed that the initial lesions in infected mice protected with either LPS or D-LPS were composed of self-limiting abscesses which transformed into granulomas as the animals recovered. It is suggested that D-LPS may be modified to become a highly effective, non-toxic salmonella vaccine.
Department of Microbiology and Immunology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
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D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D006099:Granuloma / Q000473:pathology; D008070:Lipopolysaccharides / Q000276:immunology*; D008099:Liver / Q000473:pathology; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D012481:Salmonella Infections, Animal / Q000382:microbiology / Q000517:prevention & control*; D012486:Salmonella typhimurium / Q000276:immunology / Q000472:pathogenicity*; D014774:Virulence
D001428:Bacterial Vaccines; D008070:Lipopolysaccharides