[Effects of thrombin produced in blood prior to mixing with anticoagulant on the platelet function and blood coagulation tests].
The amount of thrombin produced in the withdrawn blood of healthy donors prior to mixing with anticoagulants was estimated with thrombin-antithrombin III complex (TAT) methods. No samples showed production of thrombin in the blood mixed with anticoagulants within 2 min. After 3 min or more incubation, four donors out of 14 showed elevation of TAT, and a technician could suspect trouble only in a case during taking blood. Although two samples with low thrombin production (5.8, 12 ng/ml, normal: less than 5 ng/ml) showed no changes of APTT or prothrombin time, samples with high thrombin production (74, 98 ng/ml) showed 1.4-2 sec shortening of APTT and 0.8-1 sec shortening of prothrombin time. Platelet counts and platelet volume showed no remarkable changes even when samples with large amount of thrombin were suspended in tubes containing EDTA-2 K. Plasma beta-thromboglobulin concentration was elevated in the blood containing high amount of thrombin and close correlation was observed amount of thrombin in the blood and the amount of plasma beta-thromboglobulin (r = 0.90, P value less than 0.001). Platelet aggregation induced by collagen was not influenced by amount of thrombin produced. ADP-induced aggregation was suppressed in 3 out of 4 cases with thrombin production although no close correlation was observed between maximal aggregation rate and the concentration of thrombin. As improper handling of blood samples including withdrawal of blood leads to misunderstanding of test results. So, blood samples only for these sensitive tests should be taken and mix with anticoagulants within 2 min.
Department of Hematologic Laboratory, Toranomon Hospital, Tokyo.
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Sato
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Tsukada
D000293:Adolescent; D000328:Adult; D000925:Anticoagulants; D001780:Blood Coagulation Tests; D001800:Blood Specimen Collection; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010974:Platelet Aggregation; D013917:Thrombin / Q000096:biosynthesis / Q000502:physiology*
D000925:Anticoagulants; D013917:Thrombin