Comparison of subcellular proteins of normal prostate, benign prostatic hypertrophy, and prostatic cancer: presence of BPH-associated nonhistone proteins.
Proteins in the cytosol, postnuclear particulate, and nuclear fractions from seven specimens of normal prostate from bladder cancer patients, 14 specimens of benign hypertrophic prostate (BPH), and three specimens of cancerous prostate were analyzed and compared by SDS-polyacrylamide slab gel electrophoresis. Abundant protein species in the cytosol fractions were 60K (species having a molecular weight of about 60,000) and 42K; their relative contents were about 35% for 60K and about 12% for 42K. In the postnuclear particulate fraction, 42K was the most abundant (about 10% of the total). The contents of these major protein species were similar in specimens of normal and diseased prostates. In addition, there are marked similarities in the electrophoretic patterns for all the protein (24-29 species) in the cytosol and postnuclear particulate fractions of the human prostate, except for four minor species in the cytosol fraction. Of the nuclear proteins, the content of core histones (H2A, H2B, H3, and H4) was fundamentally similar among all the specimens, whereas the content of H1 histone was different from one specimen to another. The most remarkable and significant difference was that the 42K-NHP (nonhistone protein having a molecular weight of about 42,000), 55K-NHP, and 190K-NHP concentrations were significantly higher in BPH than in normal and cancerous prostates.
F
F
Wada
N
N
Nishi
Y
Y
Tanaka
Y
Y
Muguruma
K
K
Tanaka
M
M
Usami
T
T
Kotake
S
S
Matsui
A A
AA
Sandberg
Y
Y
Matuo
D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis*; D003600:Cytosol / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008297:Male; D011467:Prostate / Q000032:analysis* / Q000648:ultrastructure; D011470:Prostatic Hyperplasia / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate; D013347:Subcellular Fractions / Q000032:analysis
D002868:Chromosomal Proteins, Non-Histone; D012967:Sodium Dodecyl Sulfate