Hormonal activation of the adenylyl cyclases of the rat and human prostate gland.
The rat ventral prostate and the human hyperplastic prostate contain adenylyl cyclases which can be activated by a variety of neurotransmittors, including vasoactive intestinal peptide (VIP), beta 2 adrenergic agonists, and dopamine. In both species the response to VIP was predominantly localized to the epithelial fraction. In the human tissue activation of the enzyme could also be achieved with prostaglandin E1 (PGE1) and an alpha 2 adrenergic agonist both associated with the stromal compartment. Castration in the rat caused a marked reduction in the basal activity of the enzyme and the maximal level of the hormone-stimulated response per cell (per mg DNA), but had only minor effects on the pattern of activation when expressed per mg membrane protein. Androgen treatment (dihydrotestosterone propionate, 2.5 mg/day) prevented the castration effects. Estrogen treatment (estradiol benzoate, 125 micrograms/day) could not prevent the castrational changes but maintained enzyme activity at a level above that of the castrate. There were no major qualitative differences in the pattern of activation of the cyclase between the different lobes of the rat prostate and the seminal vesicle.
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Gordeladze
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Attramadal
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000527:Alprostadil / Q000494:pharmacology; D000818:Animals; D003000:Clonidine / Q000494:pharmacology; D004298:Dopamine / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D006632:Histamine / Q000494:pharmacology; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D009919:Orchiectomy; D011412:Propanolamines / Q000494:pharmacology; D011467:Prostate / Q000201:enzymology*; D011470:Prostatic Hyperplasia / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D012669:Seminal Vesicles / Q000201:enzymology; D013726:Terbutaline / Q000494:pharmacology; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
D011412:Propanolamines; D014660:Vasoactive Intestinal Peptide; C026777:ICI 118551; D006632:Histamine; D000262:Adenylyl Cyclases; D000527:Alprostadil; D007545:Isoproterenol; D003000:Clonidine; D013726:Terbutaline; D004298:Dopamine