Immunity to herpes simplex virus: virus reactivation modulates lymphokine activity.
Cell-mediated immunity to herpes simplex virus (HSV) was studied in patients with recurrent disease and in seropositive controls. Supernatants from cultures of peripheral blood mononuclear cells (PBLs) obtained during recrudescence (zero to three days after onset of lesions), convalescence (four to 14 days), and quiescence (greater than 14 days) were assayed for their ability to augment natural killer activity of normal PBLs against K562 target cells. Supernatants obtained during recrudescence, but not those obtained during convalescence, quiescence, or from seropositive controls, failed to augment natural killer activity but contained interferon and interleukin-2. Dialysis restored the ability of the supernatants to augment natural killer activity, and herpesvirus [UV-HSV-2(G)] stimulation of recrudescent PBLs in the presence of 2 X 10(6) M indomethacin abrogated suppression of natural killer-enhancing activity. However, similar levels of prostaglandin E were observed in supernatants of PBLs collected throughout the disease cycle from individuals with recurrent disease or from seropositive controls. The data suggest that reactivation of latent HSV is associated with the induction of a dialyzable factor(s) that interferes with interferon or interleukin-2-mediated natural killer enhancement or both. Prostaglandin E is necessary but insufficient for suppression of natural killer-enhancing activity.
J F
JF
Sheridan
M
M
Beck
L
L
Aurelian
M
M
Radowsky
D000328:Adult; D002478:Cells, Cultured; D005260:Female; D006558:Herpes Genitalis / Q000276:immunology* / Q000382:microbiology; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007372:Interferons / Q000502:physiology*; D007376:Interleukin-2 / Q000502:physiology*; D007694:Killer Cells, Natural / Q000276:immunology*; D008222:Lymphokines / Q000502:physiology*; D008297:Male; D011458:Prostaglandins E / Q000502:physiology*; D012008:Recurrence; D018139:Simplexvirus / Q000276:immunology / Q000502:physiology
D007376:Interleukin-2; D008222:Lymphokines; D011458:Prostaglandins E; D007372:Interferons; D007213:Indomethacin