Improved high-speed counter-current chromatograph with three multilayer coils connected in series. II. Separation of various biological samples with a semi-preparative column.
The semipreparative capability of the newly developed high-speed counter-current chromatograph equipped with a set of three multilayer coils has been demonstrated in separations of a variety of biological samples including triterpenoic acids, indole auxins, bacitracin, flavonoids and tetracycline derivatives, each with a suitable two-phase solvent system. The sample quantities ranging from 50 to 500 mg were efficiently separated within a few hours. The separation of tetracycline derivatives was remarkably improved by adding ammonium acetate to the solvent system.
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Laboratory of Technical Development, National Heart, Lung, and Blood Institute, Bethesda, MD 20892.
Oka
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Lee
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D001414:Bacitracin / Q000302:isolation & purification; D002845:Chromatography / Q000295:instrumentation* / Q000379:methods; D007210:Indoleacetic Acids / Q000302:isolation & purification; D010946:Plants, Medicinal; D010947:Plants, Toxic; D031955:Rhamnus / Q000378:metabolism; D013754:Tetracyclines / Q000302:isolation & purification
D007210:Indoleacetic Acids; D013754:Tetracyclines; D001414:Bacitracin