Tumor growth inhibitory activity of a lymphocyte blastogenesis inhibitory factor.
A lymphocyte blastogenesis inhibitory factor (LBIF) has been characterized as an immunoregulatory molecule, especially on the T-lymphocyte proliferation. Using fast protein liquid chromatography-purified LBIF, we examined the effect of LBIF on the proliferation of various 18 tumor cell lines in vitro in comparison with those of interferon-alpha, interferon-gamma, tumor necrosis factor-alpha, transforming growth factor-beta 1, or interleukin 1 alpha/beta. We showed here that LBIF strongly inhibited the proliferation of various tumor cell lines irrespective of cell lineage or species. LBIF was effective on a wider spectrum of tumor cell lines than other cytokines tested here. The inhibition resulted from cytotoxic or cytostatic effects, depending on individual characteristics of tumor cell lines. Five cell lines showed insensitivity against LBIF activity, suggesting a plausible involvement of LBIF receptor molecules to transduce LBIF signals. These results suggest that LBIF may play important roles in regulating cell growth.
Institute of Immunological Science, Hokkaido University, Sapporo, Japan.
K
K
Sugimura
T
T
Ohno
S
S
Fukuda
Y
Y
Wada
T
T
Kimura
I
I
Azuma
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D001685:Biological Factors / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D016207:Cytokines; D004305:Dose-Response Relationship, Drug; D006131:Growth Inhibitors / Q000494:pharmacology*; D006801:Humans; D008222:Lymphokines / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects*
D000276:Adjuvants, Immunologic; D001685:Biological Factors; D016207:Cytokines; D006131:Growth Inhibitors; D008222:Lymphokines