Anti-MAG antibody and antibody complexes: detection by radioimmunoassay.
A radioimmunoassay (RIA) for measuring isotype-specific antibodies to the myelin-associated glycoprotein (MAG) was developed using radiolabeled CNS MAG in a double-antibody precipitation system. Anti-MAG activity was detected by RIA only in patients with neuropathy and anti-MAG M proteins. Anti-MAG IgM or IgG antibodies were not detected in serum of patients with Guillain-Barré syndrome, chronic relapsing polyneuritis, or multiple sclerosis (MS). Some patients with anti-MAG IgM M proteins also had complexes of IgG or IgA bound to the M protein. In one patient, anti-CNS MAG activity was detected by RIA, but not by ELISA or immunoblot. Anti-MAG antibody activity in patients with neuropathy seems to be isotypically restricted, and there is no evidence for antibody reactivity to MAG in other demyelinating diseases.
Nobile-Orazio
E
E
McIntosh
C
C
Latov
N
N
D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D002493:Central Nervous System Diseases / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007163:Immunosorbent Techniques; D009185:Myelin Proteins / Q000032:analysis* / Q000276:immunology; D018992:Myelin-Associated Glycoprotein; D010523:Peripheral Nervous System Diseases / Q000276:immunology; D011863:Radioimmunoassay
D000888:Antibodies, Anti-Idiotypic; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein