Studies on sperm capacitation using monoclonal anti-body--disappearance of an antigen from the anterior part of mouse sperm head.
C57BL/6 mice were immunized with cauda epididymal sperm from syngenic mice. A monoclonal antibody (TSC4) was obtained using a hybridization method with myeloma (P3U1) cells. The antibody was examined for its reactivity to sperm from different regions of the reproductive tract of male mice. The antigen was recognized only when the sperm reached the corpus epididymis. The area of antigen present on the sperm membrane was topographically restricted to the anterior part of sperm head. The antigen remained on the sperm after washing with phosphate buffered saline, disappearing, however, when sperm was capacitated.
M
M
Okabe
K
K
Takada
T
T
Adachi
Y
Y
Kohama
T
T
Mimura
S
S
Aonuma
D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis*; D002460:Cell Line; D005260:Female; D005307:Fertilization in Vitro; D005455:Fluorescent Antibody Technique; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013075:Sperm Capacitation; D013077:Sperm Head / Q000276:immunology*; D013094:Spermatozoa / Q000276:immunology*; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D000941:Antigens