Use of superparamagnetic particles for isolation of cells.
This report describes the preparation and characterization of synthetic ferritin-like particles produced by precipitation of magnetite from a mixture of ferrous and ferric ions in the presence of dextran. The 3-nm diameter particles, containing magnetite cores surrounded by chemisorbed dextran, had a magnetization of 46.7 emu/g of iron with Mössbauer quadrupole splitting of 2 delta = 0.76 mm/s. The application of these particles as a laboratory reagent for isolation of Legionella from other water bacteria was successfully tested. A 400-fold enrichment for Legionella was obtained.
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Kronick
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Gilpin
D002469:Cell Separation / Q000379:methods*; D003911:Dextrans; D005293:Ferritins; D052203:Ferrosoferric Oxide; D005417:Flavobacterium / Q000302:isolation & purification; D007501:Iron; D007505:Iron-Dextran Complex; D007875:Legionella / Q000302:isolation & purification*; D008280:Magnetics; D008854:Microscopy, Electron; D010087:Oxides
D003911:Dextrans; D010087:Oxides; D007505:Iron-Dextran Complex; D005293:Ferritins; D007501:Iron; D052203:Ferrosoferric Oxide