Expression of the cytokeratin endo A gene during early mouse embryogenesis.
Expression of cytokeratin endo A has been analyzed during mouse blastocyst formation and embryonal carcinoma cell differentiation. To study the regulation of endo A expression, nuclease S1 mapping experiments have been performed on RNA extracted from two-cell to 7.5-day embryos. Low levels of endo A mRNA begin to be detectable in eight-cell embryos. The amount of this mRNA increases at the blastocyst stage, suggesting that endo A expression is regulated at the mRNA level during blastocyst formation. At this stage, in situ hybridization studies show that endo A mRNA is present in the trophectoderm but not in the inner cell mass. In 7.5-day embryos, endo A mRNAs are also detectable in the endoderm layer and in the amnion.
P
P
Duprey
D
D
Morello
M
M
Vasseur
C
C
Babinet
H
H
Condamine
P
P
Brûlet
F
F
Jacob
D000818:Animals; D001755:Blastocyst / Q000502:physiology / Q000648:ultrastructure; D047108:Embryonic Development; D005260:Female; D005786:Gene Expression Regulation; D007633:Keratins / Q000235:genetics* / Q000378:metabolism; D051379:Mice / Q000196:embryology*; D008815:Mice, Inbred Strains; D009024:Morphogenesis; D009028:Morula / Q000502:physiology / Q000648:ultrastructure; D009693:Nucleic Acid Hybridization; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics
D012333:RNA, Messenger; D007633:Keratins