Antibodies to spermatozoa and seminal plasma antigens detected by various enzyme-linked immunosorbent (ELISA) assays.
Various ELISA methods have been applied by different research centers to test the efficiency of this approach for the diagnosis of sperm-immune infertility cases. The antigens used were either whole spermatozoa or solubilized spermatozoal membrane preparations and were immobilized on microtiter plates, except in one case where plastic beads were employed. Polyvalent second antibodies or protein-A labelled with enzymes served as tracers. A high frequency of positive sera was found in all groups including fertile controls with tests using whole spermatozoa as antigen. The methods using solubilized antigen preparations showed fewer positives on the whole and correlated better with the various clinical categories of the WHO sera. Whilst there was some agreement in the results between the various laboratories on a few sera, most of the positive sera found by one laboratory were reported as negative by others. More investigative work is needed to improve reproducibility between different laboratories and to reduce non-specific reactions with normal controls. A more precise definition of the proper cut-off levels for positives and negatives is also needed. Despite these short-comings, the development of an ELISA for the diagnosis of sperm-immune infertility cases seems to be justified in the long term.
L
L
Mettler
A B
AB
Czuppon
N
N
Alexander
M
M
D'Almeida
G G
GG
Haas
T
T
Hjort
J
J
Møller Jensen
R
R
Ing
W R
WR
Jones
S X
SX
Wang
D000906:Antibodies / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes / Q000032:analysis; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000097:blood / Q000276:immunology; D007248:Infertility, Male / Q000097:blood / Q000276:immunology; D008297:Male; D012661:Semen / Q000276:immunology*; D013094:Spermatozoa / Q000276:immunology*; D014944:World Health Organization
D000906:Antibodies; D000939:Epitopes