Fluorescence in the tandem scanning microscope.
Our studies have shown that the fluorescence mode can be used to good effect in both tandem scanning microscopes (TSM: direct view confocal microscopes) as well as confocal scanning laser microscopes (CSLM). Applications are presented which show that the two great advantages of TSM are real-time viewing and real colour, which allow faster use and interpretation. CSLM are complementary, not competitive, being currently more sophisticated for low-level fluorescence work. This is equally possible with available TSM, but requires further development using CCD cameras and image-processing systems.
Department of Anatomy and Developmental Biology, University College London, U.K.
A
A
Boyde
S J
SJ
Jones
M L
ML
Taylor
L A
LA
Wolfe
T F
TF
Watson
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