Native and chemically modified porin channels from Salmonella typhi Ty2 in planar lipid bilayers.
Native porins, from Salmonella typhi Ty2 outer membrane, and porins alkylated with pyridoxal phosphate (Plp) were studied in planar lipid bilayers. The conductance of bilayers exposed to native or chemically modified porins increases in discrete jumps. Conductance histograms for native porins displayed two major peaks at 1.7 and 6.7 nS (in 0.5 M KCl). On the other hand, Plp-treated porins exhibited a single major peak at 1 nS. The relation between bilayer conductance and native porin concentration was linear. However, this relation became logarithmic in the presence of modified porins. The results support the notion that alkaline reduction of S. typhi Ty2 porins with Plp dissociates porin channel trimers in a reversible fashion.
Labarca
P
P
Lobos
S
S
Calderón
I
I
Mora
G
G
D000478:Alkylation; D001425:Bacterial Outer Membrane Proteins / Q000502:physiology*; D004553:Electric Conductivity; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D008051:Lipid Bilayers / Q000378:metabolism*; D018272:Porins; D011732:Pyridoxal Phosphate / Q000494:pharmacology; D012485:Salmonella typhi / Q000032:analysis*
D001425:Bacterial Outer Membrane Proteins; D007473:Ion Channels; D008051:Lipid Bilayers; D018272:Porins; D011732:Pyridoxal Phosphate