Ethanol inhibition of thrombus formation on collagen-coated glass.
Epi-fluorescent video microscopy was used to evaluate the effect of ethanol on platelet mural thrombus formation. Whole blood, treated with ethanol, was perfused over collagen coated glass in a parallel-plate flow chamber at a shear rate of 1,000/s. Digital image processing and photodiode measurements were used to analyze the dynamics of thrombus growth on this surface. Ethanol concentrations as low as 0.02% v/v were found to inhibit 45 +/- 23% (+/- S.D.) of normal platelet accumulation on the slide while 0.2% v/v ethanol effected an 82 +/- 15% inhibition of mural thrombus formation. While platelet adhesion to the collagen surface appeared unaffected by ethanol concentrations up to 0.1% v/v, 0.2% v/v ethanol had an effect on adhesion as well as aggregation. These results imply that low ethanol concentrations inhibit the formation of mural thrombi in a model of a damaged blood vessel at physiological shear rates. This inhibition would not be detected in systems which measure bulk aggregation, e. g. in aggregometric determinations.
Owens
C K
CK
Department of Chemical Engineering, Rice University, Houston, Texas 77251.
McIntire
L V
LV
Lasslo
A
A
D003094:Collagen; D000431:Ethanol / Q000494:pharmacology*; D005898:Glass; D006801:Humans; D066298:In Vitro Techniques; D010973:Platelet Adhesiveness / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors; D010976:Platelet Count / Q000187:drug effects; D013927:Thrombosis / Q000517:prevention & control*; D014741:Video Recording
D010975:Platelet Aggregation Inhibitors; D000431:Ethanol; D003094:Collagen