Purification and characterization of the major envelope glycoprotein of simian foamy virus type 1.
Simian foamy virus type 1 (SFV-1), the prototype of the Spumavirinae, was subjected to disruption and serial purification procedures. Separation of SFV-1 envelope components from viral cores was verified by electron microscopy, density gradient centrifugation and polyacrylamide gel electrophoresis. After affinity chromatography of the envelope polypeptides on a concanavalin A-Sepharose column, a highly purified 70 000 mol. wt. protein was recovered. Glycosylation of this gp70 was confirmed by glucosamine labelling. Immunological studies with anti-SFV antisera confirmed the type-specificity of this envelope gp70.
A B
AB
Benzair
A
A
Rhodes-Feuillette
J
J
Lasneret
R
R
Emanoil-Ravier
J
J
Périès
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D002460:Cell Line; D002500:Centrifugation, Isopycnic; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D005944:Glucosamine / Q000378:metabolism; D000882:Haplorhini / Q000382:microbiology; D005779:Immunodiffusion; D008970:Molecular Weight; D012190:Retroviridae / Q000032:analysis*; D016092:Spumavirus / Q000032:analysis* / Q000276:immunology / Q000648:ultrastructure; D014759:Viral Envelope Proteins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*
D000956:Antigens, Viral; D000939:Epitopes; D014759:Viral Envelope Proteins; D005944:Glucosamine