Partial purification of an adenine-dependent serum factor which retards the reactivation of lens epithelial cells in primary culture by inhibiting protein synthesis and cell spreading.
A low molecular weight factor has been partially purified from bovine serum which, in combination with adenine and, to a lesser degree, also with hypoxanthine and guanine, retards the reactivation of eye lens epithelial cells in primary culture. While the factor as well as adenine alone are without effect, in combination they strongly inhibit protein synthesis and spreading of the cells. It is concluded that the partially purified factor is identical with a previously described serum factor which mediates remarkable inhibiting effects of C6-substituted purines on protein-, RNA- and DNA-synthesis of lens epithelial cells in primary culture.
Institute of Biochemistry, Martin Luther University, GDR, Halle, Saale.
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Glässer
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Iwig
D000225:Adenine / Q000494:pharmacology*; D000818:Animals; D002417:Cattle / Q000097:blood; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D004357:Drug Synergism; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D005136:Eye Proteins / Q000096:biosynthesis; D006131:Growth Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D006147:Guanine / Q000494:pharmacology; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000494:pharmacology; D007908:Lens, Crystalline / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011500:Protein Synthesis Inhibitors / Q000302:isolation & purification / Q000494:pharmacology
D005136:Eye Proteins; D006131:Growth Inhibitors; D007042:Hypoxanthines; D011500:Protein Synthesis Inhibitors; D019271:Hypoxanthine; D006147:Guanine; D000225:Adenine