Inhibition by forskolin of cytosolic calcium rise, shape change and aggregation in quin2-loaded human platelets.
The adenylate cyclase stimulator forskolin was used to study the inhibitory effects of elevated cAMP on the activation of washed human platelets loaded with the fluorescent Ca2+ indicator quin2. In the presence of 10 microM isobutylmethylxanthine forskolin inhibited rises in [Ca2+]i evoked by thrombin and platelet-activating factor (PAF) due to both Ca2+ influx and release from internal stores with similar potency. Aggregation evoked by thrombin and PAF was suppressed whilst partial shape-change persisted, even in the absence of a measurable rise in [Ca2+]i. Forskolin did not affect the rise in [Ca2+]i evoked by Ca2+ ionophore; aggregation was suppressed but shape-change persisted.
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D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000634:Aminoquinolines; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D002118:Calcium / Q000097:blood*; D005576:Colforsin; D000242:Cyclic AMP / Q000097:blood; D003600:Cytosol / Q000378:metabolism; D004224:Diterpenes / Q000494:pharmacology*; D005456:Fluorescent Dyes; D006801:Humans; D010972:Platelet Activating Factor / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects*; D013917:Thrombin / Q000494:pharmacology
D000634:Aminoquinolines; D004224:Diterpenes; D005456:Fluorescent Dyes; D010972:Platelet Activating Factor; D005576:Colforsin; D000242:Cyclic AMP; D013917:Thrombin; C035739:Quin2; D002118:Calcium; D015056:1-Methyl-3-isobutylxanthine