Influence of interleukin-1 beta on the secretion of oxytocin and vasopressin from the isolated rat neurohypophysis.
The effect of the cytokine interleukin-1 beta on the secretion of oxytocin and vasopressin from electrically stimulated rat neurohypophysis was examined in vitro. The release of oxytocin and vasopressin was concentration-dependently increased by interleukin-1 beta in the concentration range from 4.4 pM to 440 pM. The effect of interleukin-1 beta on oxytocin secretion was less intense as compared to vasopressin. After 440 pM interleukin-1 beta the electrically evoked release of oxytocin was increased about 22% and had not reached its maximum. The vasopressin response was maximal after 44 pM interleukin-1 beta, the response being increased 43% compared to control. No trace of interleukin-1 beta was found in the posterior pituitary (less than 350 pmol/lobe, radioimmunoassay). The results indicate that interleukin-1 beta might be involved the regulation of oxytocin and vasopressin at the pituitary level.
Department of Biological Sciences, Royal Danish School of Pharmacy, Copenhagen.
J D
JD
Christensen
E W
EW
Hansen
B
B
Fjalland
D000818:Animals; D004558:Electric Stimulation; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000032:analysis / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D010121:Oxytocin / Q000032:analysis / Q000378:metabolism*; D010904:Pituitary Gland, Posterior / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D014667:Vasopressins / Q000032:analysis / Q000378:metabolism*
D007375:Interleukin-1; D014667:Vasopressins; D010121:Oxytocin