Casein expression in cytotoxic T lymphocytes.
A cDNA that expresses a mRNA restricted to cytotoxic T lymphocytes (CTL) and mammary tissue has been isolated and characterized. The deduced amino acid sequence from this cDNA shows extensive homology with the previously reported amino acid sequence for rat alpha-casein. Indeed, the presence of a six-residue-repeated motif that is specific for rodent alpha-caseins strongly supports the identification of this cDNA as mouse alpha-casein. Northern (RNA) blot analysis of many hematopoietic cell types revealed that this gene is restricted to CTL, being expressed in four of six CTL lines examined. Furthermore, CTL that express this gene were also found to express other members of the casein gene family, such as beta- and kappa-casein. These results suggest that caseins may be important in CTL function, and their potential role in CTL-mediated lysis is discussed.
Grusby
M J
MJ
Department of Cancer Biology, Harvard School of Public Health, Boston, MA 02115.
Mitchell
S C
SC
Nabavi
N
N
Glimcher
L H
LH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002364:Caseins / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005260:Female; D015723:Gene Library; D008297:Male; D008321:Mammary Glands, Animal / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000378:metabolism*
D002364:Caseins; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D004247:DNA