[Comparison of various methods of cryopreservation of human pre-embryos].
A human egg and embryo freezing program for non-fertilized eggs and spare embryos has been established in our in vitro fertilization clinic. In the following we report on our first experience with different methods of human pre-embryo cryopreservation. In a simple evaluation we have compared different conventional freezing techniques using cryoprotectant substances such as glycerol, DMSO and 1.2 propanediol and an "ultrarapid" technique. So far we have achieved 6 normal ongoing and 2 biochemical pregnancies out of a total number of 61 embryo transfers. This equals 9.8% pregnancy rate per embryo transfer. Two methods seem preferable, i.e. sucrose and 1.2 propandiol which has the highest success rate and the ultrarapid method which gives a good rate of embryo survival on the one hand and a simple handling on the other.
Institut für Sterilitätsbetreuung, Wien.
M
M
Putz
F
F
Kunz
C
C
Hochfellner
U
U
Ferstl
P
P
Kemeter
W
W
Feichtinger
D004624:Embryo Transfer / Q000379:methods*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D005500:Follow-Up Studies; D005615:Freezing; D006801:Humans; D011247:Pregnancy; D014021:Tissue Preservation / Q000379:methods*
Vergleich verschiedener Methoden der Kryokonservierung humaner Prä-Embryonen.