Measurement of myelin basic protein by radioimmunoassay in closed head trauma, multiple sclerosis and other neurological diseases.
A double antibody sequential radioimmunoassay for human myelin basic protein (MBP) has been developed. The assay utilizes a rabbit antibody to human MBP and purified rabbit MBP as the radiolabelled antigen. This assay was used to analyze cerebrospinal fluid (CSF) from 22 patients with severe head injury, 61 other cases of various neurological disorders, and 106 normal controls. The results showed that closed head trauma caused moderate to severe elevations in CSF MBP, and elevated CSF MBP was detectable in several diseases which involve CNS myelin.
Mukherjee
A
A
Vogt
R F
RF
Linthicum
D S
DS
D006259:Craniocerebral Trauma / Q000134:cerebrospinal fluid*; D006801:Humans; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid*; D004676:Myelin Basic Protein / Q000134:cerebrospinal fluid*; D009422:Nervous System Diseases / Q000134:cerebrospinal fluid*; D011863:Radioimmunoassay
D004676:Myelin Basic Protein