False positive reaction for carcinoembryonic antigen in Paget cells. Immunohistochemical observation.
Paget cells from cases of mammary and extramammary Paget's disease were examined for carcinoembryonic antigen (CEA) and CEA-related antigens by the immunoperoxidase method. Paget cells showed a conspicuous positive reaction with antiserum to CEA, but were negative when nonspecific cross-reacting-antigen (NCA)-absorbed antiserum to CEA, or a monoclonal antibody to CEA was used as the detecting agents. Paget cells may contain large amounts of NCA antigen or CEA-related substances.
S
S
Sumitomo
Y
Y
Tatemoto
S
S
Fukui
T
T
Nakamura
S
S
Fukushima
N
N
Ito
M
M
Mori
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen / Q000032:analysis*; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000032:analysis*; D003429:Cross Reactions; D005189:False Positive Reactions; D006801:Humans; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D010145:Paget Disease, Extramammary / Q000032:analysis*; D010144:Paget's Disease, Mammary / Q000032:analysis*; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D007106:Immune Sera