Antigenic properties and protective capacity of a cyclic peptide corresponding to site A of influenza virus haemagglutinin.
Two cyclic peptide analogues corresponding to residues 139-146 (site A) of influenza A virus haemagglutinin (strain X31) were synthesized. The ability of these peptides to react with anti-influenza virus antibodies was found to depend on the conformation of the loop and on the orientation in which the peptide was presented to antibodies. Antibodies raised to the peptides were able to bind in ELISA with influenza virus antigen that had been allowed to dry on the microtitre plate. When OF1 mice were immunized with cyclic peptides, approximately 80% of the animals were protected against an intranasal challenge with influenza virus.
Muller
S
S
Laboratoire d'Immunochimie, Institut de Biologie Moléculaire et Cellulaire, Strasbourg, France.
Plaué
S
S
Samama
J P
JP
Valette
M
M
Briand
J P
JP
Van Regenmortel
M H
MH
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral / Q000276:immunology* / Q000627:therapeutic use; D007106:Immune Sera / Q000276:immunology; D007114:Immunization; D009980:Influenza A virus / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009976:Orthomyxoviridae Infections / Q000517:prevention & control; D010456:Peptides, Cyclic / Q000138:chemical synthesis / Q000276:immunology* / Q000627:therapeutic use; D011487:Protein Conformation; D013329:Structure-Activity Relationship
D000914:Antibodies, Viral; D000956:Antigens, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral; D007106:Immune Sera; D010456:Peptides, Cyclic