Feprazone: an inducer of the P450 II B family of proteins in the rat.
The ability of feprazone to induce the hepatic microsomal mixed-function oxidases was investigated in the rat, with emphasis being placed on the nature of the cytochrome P-450 family induced. Treatment with feprazone enhanced the p-hydroxylation of aniline and the dealkylations of benzphetamine and pentoxyresorufin but had no effect on the O-deethylation of ethoxyresorufin. The same treatment had no major effect on total cytochrome P-450 levels but increased the spectral interaction of metyrapone with reduced cytochrome P-450. Immunoblots employing monospecific polyclonal antibodies revealed that feprazone induces the apoprotein levels of the P450 II B, but not of the P450 I, family. It is concluded that feprazone is an inducer of the rat hepatic mixed-function oxidase system showing selectivity toward the P450 II B family.
Department of Biochemistry, University of Surrey, Guildford, United Kingdom.
J N
JN
Smith
A D
AD
Ayrton
J
J
Chown
D F
DF
Lewis
C
C
Ioannides
D000818:Animals; D000906:Antibodies; D001835:Body Weight / Q000187:drug effects; D003198:Computer Simulation; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D003600:Cytosol / Q000201:enzymology; D004790:Enzyme Induction; D011298:Feprazone / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000378:metabolism; D015151:Immunoblotting; D008099:Liver / Q000033:anatomy & histology / Q000201:enzymology / Q000648:ultrastructure; D008297:Male; D006899:Mixed Function Oxygenases / Q000096:biosynthesis; D015394:Molecular Structure; D009249:NADP / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D010653:Phenylbutazone / Q000031:analogs & derivatives*; D051381:Rats; D011919:Rats, Inbred Strains
D000906:Antibodies; D009249:NADP; D011298:Feprazone; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D005982:Glutathione Transferase; D010653:Phenylbutazone