Structure of protein-deficient 50 S ribosomal subunits. Particles without 5 S RNA-protein complex retain the L7/L12 stalk and associate with 30 S subunits.
50 S ribosomal subunit derivatives without the 5 S RNA-protein complex obtained either by splitting with EDTA or by reconstitution from the 23 S RNA and proteins have been studied by electron microscopy. Removal of the 5 S RNA-protein complex is shown to affect neither the overall morphology of the larger ribosomal subunit nor the mode of its association with the small subunit.
Selivanova
O M
OM
Gongadze
G M
GM
Gudkov
A T
AT
Vasiliev
V D
VD
D002499:Centrifugation, Density Gradient; D004492:Edetic Acid / Q000494:pharmacology; D004926:Escherichia coli / Q000032:analysis*; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012261:Ribonucleoproteins
D046911:Macromolecular Substances; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012261:Ribonucleoproteins; D004492:Edetic Acid