Increased mRNA for low density lipoprotein receptor in livers of rabbits treated with 17 alpha-ethinyl estradiol.
Pharmacologic doses of 17 alpha-ethinyl estradiol are known to increase the number of low density lipoprotein (LDL) receptors in livers of rats, thereby producing a profound fall in plasma cholesterol levels. We now report that ethinyl estradiol exerts the same effect in livers of male and female rabbits and that the increase in receptor number is correlated with a 6- to 8-fold increase in the levels of receptor mRNA. Receptor protein was measured by ligand blotting, and mRNA levels were measured by a quantitative solution hybridization/S1 nuclease protection assay using uniformly 32P-labeled single-stranded cDNA probes. These experiments demonstrate that pharmacologic induction of the mRNA for the LDL receptor in liver can lead to increased LDL receptor levels and a fall in plasma cholesterol in experimental animals.
Ma
P T
PT
Yamamoto
T
T
Goldstein
J L
JL
Brown
M S
MS
D000818:Animals; D002784:Cholesterol / Q000097:blood; D004247:DNA / Q000378:metabolism; D004997:Ethinyl Estradiol / Q000494:pharmacology*; D005260:Female; D008099:Liver / Q000032:analysis / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis*; D011817:Rabbits; D011973:Receptors, LDL / Q000032:analysis / Q000235:genetics* / Q000378:metabolism
D012333:RNA, Messenger; D011973:Receptors, LDL; D011061:Poly A; D004997:Ethinyl Estradiol; D012313:RNA; D004247:DNA; D002784:Cholesterol