In vitro translation of mRNA from the developing sciatic nerve.
Poly(A+)RNA from sciatic nerves of embryos and chicks was translated in a rabbit reticulocyte cell-free system. The 30-K mol. wt. PO protein which is the major and specific protein constituent of peripheral nervous system myelin was used as a marker of myelin synthesis in the preparations. Immunoprecipitation of total translation products with rabbit anti-PO protein antibody showed the presence of a 29K protein band on the autoradiograms. The intensity of this specific immunoprecipitated band increased significantly from very low levels with RNA from the 15-day embryonic nerve to a maximum with extracts from 1-day-old chicks. This increase parallels that of the rapid deposition of the PO protein in the developing sciatic nerve in vivo. The experiments demonstrate for the first time, that it is possible to show synthesis of PO protein in vitro using exogenous RNA as the template.
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Leblanc
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Mezei
D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D018993:Myelin P0 Protein; D009185:Myelin Proteins / Q000096:biosynthesis / Q000235:genetics*; D010525:Peripheral Nerves / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism
D018993:Myelin P0 Protein; D009185:Myelin Proteins; D012333:RNA, Messenger