Haptoglobin subtyping with anti-haptoglobin alpha chain antibodies.
Specific antibodies against the human haptoglobin (HP) alpha chain were raised and used for immunoblotting after isoelectric focusing to determine Hp alpha subtypes in a reproducible and simplified manner. By eliminating the staining of the HP beta chain, HP alpha subtypes were visualized more precisely and simply than by previously reported methods. Subtypes of a total of 1211 sera, obtained from two different populations in Japan, were examined by the new method. Four HP alpha subtypes (HP 1S-1S,2FS-1S, 2FS-2FS and 2FS-2SS) and five subtypes combined with a new variant (HP 2FS-1V1, 2V1-1S, 2FS-2V1, 2FS-2V2 and 2FS-2V3) were observed. HP 1V1 belonged to HP alpha 1, and HP 2V1, 2V2 and 2V3 belonged to HP alpha 2, and their alleles were designated HP A*1V1, HP A*2V1, HP A*2V2 and HP A*2V3, respectively. The allele frequencies were calculated to be as follows: HP A*1S, 0.2597; HP A*1V1, 0.0004; HP A*2FS, 0.7333; HP A*2SS, 0.0008; HP A*2V1, 0.0045; HP A*2V2, 0.0008; and HP A*2V3, 0.0004. The allele frequency of HP A*2SS, which is common in the European population, is less frequent than HP A*2V1 in the Japanese population.
Department of Forensic Medicine, Akita University School of Medicine, Japan.
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Shindo
D000483:Alleles; D000918:Antibody Specificity / Q000276:immunology*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D006242:Haptoglobins / Q000235:genetics / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007525:Isoelectric Focusing; D007564:Japan; D008297:Male; D010375:Pedigree
D006242:Haptoglobins