Oncofetal antigen in Xiphophorus detected by monoclonal antibodies directed against melanoma-associated antigens.
Monoclonal antibodies (MAbs) directed against Xiphophorus melanoma cells were developed and tested by indirect immunofluorescence and immunoperoxidase staining for reactivity with a panel of 15 allogeneic tissues and 12 allogeneic cell lines. The reactivity of such MAbs was restricted to melanoma cells from tumor biopsies and melanoma-derived cell lines. In addition, all embryonic cells of all histiotypes from developmental stages later than mid-organogenesis and from corresponding short term in vitro cultures reacted with these MAbs. In contrast, normal tissues and organs from adult fish displayed no reactivity, thus implying that the melanoma-associated antigens detected by the MAbs described are oncofetal antigens.
Genetisches Institut, Universität Giessen, FRG.
G
G
Clauss
C
C
Winkler
J
J
Lohmeyer
F
F
Anders
M
M
Schartl
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000302:isolation & purification; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D002460:Cell Line / Q000276:immunology; D003532:Cyprinodontiformes / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005393:Fish Diseases / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006825:Hybridomas / Q000276:immunology; D007124:Immunoenzyme Techniques; D008545:Melanoma / Q000276:immunology* / Q000662:veterinary; D051379:Mice; D008807:Mice, Inbred BALB C; D011233:Precipitin Tests; D014407:Tumor Cells, Cultured / Q000276:immunology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; C031192:oncofetal antigens