[Changes in subunit conformation and their reciprocal configuration in the transition from the pretranslocated to the posttranslocated state].
RNA-protein contacts in pretranslocated and posttranslocated states of E. coli ribosomes have been determined by means of UV-induced cross-linking. In the two functional states as well as in free 70C ribosome, the same proteins are involved in RNA-protein intersubunit contacts, located in the region of L1 protuberance (left side of 70S ribosome). The transition from pre- to posttranslocated state is accompanied by disappearance of RNA-protein contacts in the region of L7/L12 stalk. This favours the locking-unlocking model of the translating ribosome.
G G
GG
Abdurashidova
E A
EA
Tsvetkova
E I
EI
Budovskiĭ
D004926:Escherichia coli / Q000378:metabolism* / Q000528:radiation effects; D011485:Protein Binding / Q000528:radiation effects; D012329:RNA, Bacterial / Q000378:metabolism* / Q000528:radiation effects; D012269:Ribosomal Proteins / Q000378:metabolism* / Q000528:radiation effects; D014466:Ultraviolet Rays
D012329:RNA, Bacterial; D012269:Ribosomal Proteins
Izmenenie konformatsii subchastits i ikh vzaimnogo raspolozheniia pri perekhode ot pretranslotsirovannogo sostoianiia k posttranslotsirovannomu.