The importance of the pathogenic 16/6 idiotype in the induction of SLE in naive mice.
We have previously demonstrated the pathogenicity of the common anti-DNA idiotype designated 16/6 Id. Immunization of naive mice with the 16/6 Id induced SLE-like disease characterized by serological (e.g. anti-dsDNA and anti-Sm auto-antibodies), clinical (increased ESR, leucopenia and proteinuria), and pathological (16/6 Id deposition in kidneys) parameters. To elucidate further the role of the 16/6 Id in SLE induction the following studies were carried out: BALB/c mice were immunized with SA-1, a human anti-DNA monoclonal antibody carrying the 16/6 Id; TB-68, a mouse monoclonal anti-tuberculosis (TB) glycolipid, which binds dsDNA and carries the 16/6 Id; TB-72, a mouse monoclonal anti-TB glycolipid that binds DNA and does not harbour the 16/6 Id; and 4B4, a human anti-Sm antibody that carries the 16/6 Id. SLE was induced in BALB/c mice only when immunized with SA-1, TB-68, and 4B4, namely antibodies with diverse binding capacities albeit having the 16/6 Id. Our studies further support previous evidence on the pathogenic role attributed to the 16/6 Id in SLE, and suggest that SLE is most probably an idiotype-induced disease.
Department of Medicine B, Sheba Medical Center, Israel.
M
M
Blank
M
M
Krup
S
S
Mendlovic
H
H
Fricke
E
E
Mozes
N
N
Talal
A R
AR
Coates
Y
Y
Shoenfeld
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage / Q000276:immunology; D001323:Autoantibodies / Q000276:immunology*; D015153:Blotting, Western; D004247:DNA / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006439:Hemodynamics; D007124:Immunoenzyme Techniques; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007668:Kidney / Q000473:pathology; D008180:Lupus Erythematosus, Systemic / Q000209:etiology* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D007130:Immunoglobulin Idiotypes; D004247:DNA