Brugia pahangi: stage-specific antigens on microfilariae detected by serum from infected jirds or by monoclonal antibodies.
Experiments were carried out to determine whether there are stage-specific antigens on microfilariae of Brugia pahangi, using sera from Mongolian jirds infected with B. pahangi and monoclonal antibodies against microfilariae of B. pahangi. These studies showed that microfilariae have both stage-specific and nonspecific antigens. The nonspecific antigens were also present on adult worms and on infective larvae. Among monoclonal antibodies, 6 out of 14 clones produced antibodies against the microfilarial stage-specific antigens, and 8 clones produced antibodies against nonspecific antigens. These monoclonal antibodies could not distinguish between adults, microfilariae, or infective larvae of B. malayi and B. pahangi.
M
M
Tomisato
H
H
Nariuchi
J
J
Ueno
S
S
Nogami
H
H
Tanaka
Y
Y
Aoki
Y
Y
Nakajima
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D000947:Antigens, Helminth / Q000032:analysis* / Q000276:immunology; D002009:Brugia / Q000254:growth & development / Q000276:immunology*; D003429:Cross Reactions; D004605:Elephantiasis, Filarial / Q000276:immunology / Q000469:parasitology; D000939:Epitopes / Q000276:immunology; D005260:Female; D005849:Gerbillinae; D007124:Immunoenzyme Techniques; D008297:Male; D013045:Species Specificity
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D000939:Epitopes