Inhibition of rabbit platelet phosphodiesterase activity and aggregation by calcium channel blockers.
Rabbit platelet cyclic AMP phosphodiesterase is inhibited by the three calcium channel blockers nifedipine, diltiazem, and verapamil with IC50's of 100 microM, 100 microM and 420 microM, respectively. Also, platelet aggregation induced by 4 microM ADP is inhibited by those compounds. Verapamil is the most potent aggregation inhibitor with an IC50 of 260 microM while diltiazem and nifedipine have IC50's of 630 microM and 840 microM, respectively. All three compounds display a maximum inhibition of 80-85%. Diltiazem and PGD2 potentiate the antiaggregatory activity of each other in that the inhibitions occurring in the presence of the combination of the two (at varying concentrations) exceed the calculated sums of the inhibitions produced by each alone. On the other hand, the antiaggregatory activities of verapamil or nifedipine, are additive with that of PGD2 in that no significant differences exist between the observed inhibitions produced by the combinations and the calculated summed values of the individual inhibitions. Our data suggest, therefore, that in addition to lowering intracellular calcium ions, which are required for platelet aggregation, the three calcium channel blockers inhibit the breakdown of cyclic AMP thereby promoting antiaggregation.
Moore
J B
JB
Fuller
B L
BL
Falotico
R
R
Tolman
E L
EL
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000037:antagonists & inhibitors / Q000097:blood*; D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001792:Blood Platelets / Q000201:enzymology*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D003600:Cytosol / Q000201:enzymology; D004110:Diltiazem / Q000494:pharmacology; D007700:Kinetics; D009543:Nifedipine / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects*; D011817:Rabbits; D013329:Structure-Activity Relationship; D014700:Verapamil / Q000494:pharmacology
D002121:Calcium Channel Blockers; D000244:Adenosine Diphosphate; D014700:Verapamil; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D004110:Diltiazem; D009543:Nifedipine; D015056:1-Methyl-3-isobutylxanthine