A radioimmunoassay for protein C.
A simple and dependable radioimmunoassay has been devised to measure protein C in human plasma. From a rabbit antiserum against human protein C, the antibodies recognizing the activation peptide region (residues 1-15) in the heavy chain of protein C were affinity purified for use in the radioimmunoassay. The radiolabelled peptide was used as a tracer. The protein C concentration in healthy subjects was 2.5 +/- 0.9 mg/l (2 SD). In warfarin treated persons the protein C level was reduced to approximately 40% of the normal level. Patients with hereditary protein C deficiency had protein C levels ranging from 0.5 mg/l to 1.6 mg/l.
K
K
Ikeda
J
J
Stenflo
D000595:Amino Acid Sequence; D000939:Epitopes / Q000276:immunology; D006023:Glycoproteins / Q000097:blood* / Q000172:deficiency / Q000276:immunology; D006801:Humans; D010455:Peptides / Q000276:immunology; D011486:Protein C; D011863:Radioimmunoassay / Q000379:methods*; D012016:Reference Values; D014859:Warfarin / Q000494:pharmacology
D000939:Epitopes; D006023:Glycoproteins; D010455:Peptides; D011486:Protein C; D014859:Warfarin