[Role of a carbohydrate component in the formation of immunochemical determinants of carcinoembryonic antigen].
Carcinoembryonic antigen (CEA) isolated from metastases of colon adenocarcinoma was subjected to deglycosylation with liquid hydrogen fluoride. The protein fraction obtained (PF CEA) was used for to prepare monospecific antiserum to PF CEA. Comparative studies of CEA, PF CEA, and non-specific cross-reacting antigen (NCA-1) have been carried out using monospecific antisera. Circular dichroism spectra of CEA and PF CEA have been studied. The data obtained suggest that some immunodominant regions of CEA are topographic, and their formation needs a specific conformation of the macromolecule, which is stabilized by the sugar moiety.
Pavlenko
A F
AF
Kurika
A V
AV
Belogortseva
N I
NI
Nabiullin
A A
AA
Ovodov
Iu S
IuS
D000230:Adenocarcinoma / Q000276:immunology; D000596:Amino Acids / Q000032:analysis; D002272:Carcinoembryonic Antigen / Q000276:immunology* / Q000302:isolation & purification; D055598:Chemical Phenomena; D002621:Chemistry; D002942:Circular Dichroism; D003110:Colonic Neoplasms / Q000276:immunology; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis*; D006601:Hexoses / Q000032:analysis / Q000276:immunology*; D006801:Humans; D005779:Immunodiffusion; D008113:Liver Neoplasms / Q000276:immunology / Q000556:secondary; D012795:Sialoglycoproteins / Q000032:analysis
D000596:Amino Acids; D002272:Carcinoembryonic Antigen; D000939:Epitopes; D006601:Hexoses; D012795:Sialoglycoproteins
Rol' uglevodnogo komponenta v formirovanii immunokhimicheskikh determinant rakovoembrional'nogo antigena.