Molecular cloning of cDNA for the human tumor-associated antigen CO-029 and identification of related transmembrane antigens.
The human tumor-associated antigen CO-029 is a monoclonal antibody-defined cell surface glycoprotein of 27-34 kDa. By using the high-efficiency COS cell expression system, a full-length cDNA clone for CO-029 was isolated. When transiently expressed in COS cells, the cDNA clone directed the synthesis of an antigen reactive to monoclonal antibody CO-029 in mixed hemadsorption and immunoblot assays. Sequence analysis revealed that CO-029 belongs to a family of cell surface antigens that includes the melanoma-associated antigen ME491, the leukocyte cell surface antigen CD37, and the Sm23 antigen of the parasitic helminth Schistosoma mansoni. CO-029 and ME491 antigen expression and the effect of their corresponding monoclonal antibodies on cell growth were compared in human tumor cell lines of various histologic origins.
Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
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Szala
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Kasai
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Steplewski
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Rodeck
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Koprowski
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Linnenbach
D000595:Amino Acid Sequence; D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000235:genetics*; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics*; D008961:Models, Structural; D008969:Molecular Sequence Data; D011487:Protein Conformation; D012689:Sequence Homology, Nucleic Acid; D059470:Tetraspanins; D014162:Transfection
D000951:Antigens, Neoplasm; D004273:DNA, Neoplasm; D008562:Membrane Glycoproteins; C065609:TSPAN8 protein, human; D059470:Tetraspanins