Digoxigenin-labeled probes can detect single-copy genes in human metaphase chromosomes.
A technique of in situ hybridization on metaphases of chromosomes by a digoxigenin-labeled probe is described. This technique was able to detect single DNA sequences of 2 and 7 kilobases. The results obtained were compared with those of a biotin streptavidin alkaline phosphatase-based detection system. The digoxigenin method was at least as efficient and sensitive as the biotin-streptavidin method.
Vernole
P
P
2nd University of Rome.
D000469:Alkaline Phosphatase; D001710:Biotin; D002871:Chromosome Banding; D002877:Chromosomes, Human / Q000032:analysis; D004247:DNA / Q000032:analysis; D004076:Digoxigenin; D004077:Digoxin / Q000031:analogs & derivatives; D005796:Genes; D005823:Genetics; D006801:Humans; D008677:Metaphase; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes
D015345:Oligonucleotide Probes; D001710:Biotin; D004077:Digoxin; D004247:DNA; D000469:Alkaline Phosphatase; D004076:Digoxigenin