An enzyme-amplified monoclonal immunoenzymometric assay for prostatic acid phosphatase.
An immunoassay for prostatic acid phosphatase is described in which a high degree of specificity for the prostatic isoenzyme, obtained by the use of monoclonal antibodies, is combined with great sensitivity, made possible by enzyme-amplified measurement of the combination of the isoenzyme with its antibody. The increase in sensitivity thus achieved is of the order of 170 times that of conventional methods of measurement. The advantages of the enzyme-amplified method have been shown to be particularly useful in detecting and monitoring small abnormalities of prostatic acid phosphatase levels in patients with prostatic cancer.
Moss
D W
DW
Self
C H
CH
Whitaker
K B
KB
Bailyes
E
E
Siddle
K
K
Johannsson
A
A
Stanley
C J
CJ
Cooper
E H
EH
D000135:Acid Phosphatase / Q000097:blood*; D000911:Antibodies, Monoclonal; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D007527:Isoenzymes / Q000097:blood*; D008297:Male; D008722:Methods; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D011467:Prostate / Q000201:enzymology*; D011471:Prostatic Neoplasms / Q000201:enzymology; D012016:Reference Values
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007527:Isoenzymes; D009243:NAD; D009249:NADP; D000135:Acid Phosphatase