[Characteristics of the phenotypic manifestation of hpt and gpt mutations blocking 6-oxypurine utilization and their effect on the expression of catabolite-sensitive genes in the cells of an Escherichia coli K-12 purine auxotroph].
The Escherichia coli purine auxotrophs with the complete block of utilization of hypoxanthine, guanine and xanthine by means of phosphoribosyltransferases, as a result of the hpt and gpt mutations, have the Rel phenotype. In the purD hpt gpt bacteria, under conditions of amino acid starvation synthesis of RNA continues and accumulation of ppGpp is not found. Upon a study of expression of the deo-operon, uridine phosphorylase and beta-galactosidase genes, data were obtained showing that activity of catabolite sensitive promoters of these genes is inhibited in the purD hpt gpt cells. Inhibition of activity of the catabolite sensitive cytP promoter in the deo-operon seems to be accompanied by an increase in activity of the preceding deoP promoter.
Brikun
I A
IA
Sukhodolets
V V
VV
D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D006147:Guanine / Q000378:metabolism; D007042:Hypoxanthines / Q000378:metabolism; D009154:Mutation; D009876:Operon; D010430:Pentosyltransferases / Q000235:genetics; D010641:Phenotype; D011687:Purines / Q000378:metabolism*; D012329:RNA, Bacterial / Q000096:biosynthesis / Q000235:genetics; D014543:Uridine Phosphorylase / Q000235:genetics; D014970:Xanthines / Q000378:metabolism
D007042:Hypoxanthines; D011687:Purines; D012329:RNA, Bacterial; D014970:Xanthines; D006147:Guanine; D010430:Pentosyltransferases; D014543:Uridine Phosphorylase
Osobennosti fenotipicheskogo proiavleniia mutatsiĭ hpt i gpt, blokiruiushchikh utilizatsiiu 6-oksipurinov, i ikh vliianie na ékspressiiu katabolitchuvstvitel'nykh genov v kletkakh purinovogo auksotrofa Escherichia coli K-12.