[Formation of mixed spheroids by a coculture of human cancerous and fibroblast cells].
The coculture of fibroblasts with cancerous cells under the conditions which lead to spheroid formation, allowed the obtention of spheroids composed of a fibroblastic core surrounded by cancerous cells. The fibroblast core was labelled by hyaluronic acid and hyaluronectin. Hyaluronic acid concentration was also measured by enzymoimmunological assay in culture medium where it was found to accumulate during spheroid growth. The composite spheroid technique is a good model system for analysis of cancer cells-fibroblasts interaction in vitro.
C
C
Chauzy
B
B
Delpech
N
N
Girard
A
A
Olivier
D002352:Carrier Proteins / Q000032:analysis; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology*; D005910:Glioma / Q000473:pathology*; D006801:Humans; D018960:Hyaluronan Receptors; D006820:Hyaluronic Acid / Q000032:analysis; D007700:Kinetics; D013997:Time Factors
D002352:Carrier Proteins; D018960:Hyaluronan Receptors; D006820:Hyaluronic Acid
Formation de sphéroïdes mixtes par une coculture de cellules cancéreuses et de fibroblastes humains.