Use of a cloned gene involved in candicidin production to discover new polyene producer Streptomyces strains.
A p-aminobenzoic synthase gene (pabS) from Streptomyces griseus IMRU 3570 involved in candicidin production was used as probe to find new aromatic polyene producing Streptomyces strains. The pab gene hybridizes with 6 out of 16 Streptomyces strains, and those strains which hybridize turned out to be polyene producers. Such strains were never before described as polyene producers.
Area de Microbiologia, Facultad de Biología, Universidad de León, Spain.
J A
JA
Gil
L M
LM
Criado
T
T
Alegre
J F
JF
Martín
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D015139:Blotting, Southern; D002174:Candicidin / Q000096:biosynthesis*; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D010957:Plasmids; D015183:Restriction Mapping; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism; D000637:Transaminases / Q000235:genetics*; D014169:Transformation, Bacterial
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D002174:Candicidin; C022309:PABA synthase; D000637:Transaminases