Anionic and neutral glutathione S-transferase isoenzymes in the freshwater worm Tubifex tubifex (O.F.M.).
The anionic and the neutral glutathione S-transferase (GST) isoenzymes from the freshwater worm Tubifex tubifex (O.F.M.) were separated in one step by chromatofocusing on a Polybuffer exchanger 94 column, eluted with Polybuffer 74. Their pI values ranged between 4.12 and 6.98, and their molecular weight between 30 000 and 38 400. The apparent Km values towards glutathione and 1-chloro-2,4-dinitrobenzene were also determined. The high number of non-cationic GST isoenzymes is unusual. Tubifex worms seems well equipped for attacking environmental pollutants.
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PJ
Dierickx
D000818:Animals; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D005618:Fresh Water; D005982:Glutathione Transferase / Q000032:analysis*; D007527:Isoenzymes / Q000032:analysis*; D007700:Kinetics; D008970:Molecular Weight; D009835:Oligochaeta / Q000201:enzymology*
D007527:Isoenzymes; D005982:Glutathione Transferase