Establishment of hybridomas secreting human monoclonal antibodies against tetanus toxin and hepatitis B virus surface antigen.
Mouse-human heterohybrids (M X H) were constructed and compared with other cell lines (human or mouse) as parental cells to obtain hybrids secreting human monoclonal antibody (MoAb). One of the M X H lines, HM-5, was far superior to the others and useful for establishing hybrids secreting human MoAb. Using HM-5 as a parental cell line, we have obtained 2 hybrids secreting human anti-tetanus toxoid MoAb with neutralizing activity and a hybrid secreting human anti-hepatitis B virus surface antigen (HBsAg) MoAb which recognizes the a-determinant of HBsAg.
Y
Y
Ichimori
K
K
Sasano
H
H
Itoh
S
S
Hitotsumachi
Y
Y
Kimura
K
K
Kaneko
M
M
Kida
K
K
Tsukamoto
D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology*; D000918:Antibody Specificity; D002460:Cell Line; D002875:Chromosomes / Q000032:analysis; D000939:Epitopes / Q000032:analysis; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007700:Kinetics; D051379:Mice; D013744:Tetanus Toxin / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006514:Hepatitis B Surface Antigens; D013744:Tetanus Toxin