Cloning and sequencing of a human pancreatic tumor mucin cDNA.
A monospecific polyclonal antiserum against deglycosylated human pancreatic tumor mucin was used to select human pancreatic mucin cDNA clones from a lambda gt11 cDNA expression library developed from a human pancreatic tumor cell line. The full-length 4.4-kilobase mucin cDNA sequence included a 72-base pair 5'-untranslated region and a 307-base pair 3'-untranslated region. The predicted amino acid sequence for this cDNA revealed a protein of 122,071 daltons containing 1,255 amino acid residues of which greater than 60% were serine, threonine, proline, alanine, and glycine. Approximately two-thirds of the protein sequence consisted of identical 20-amino acid tandem repeats which were flanked by degenerate tandem repeats and nontandem repeat sequences on both the amino-terminal and carboxyl-terminal ends. The amino acid sequence also contained five putative N-linked glycosylation sites, a putative signal sequence and transmembrane domain, and numerous serine and threonine residues (potential O-linked glycosylation sites) outside and within the tandem repeat position. The cDNA and deduced amino acid sequence of the pancreatic mucin sequence was over 99% homologous with a mucin cDNA sequence derived from breast tumor mucin, even though the native forms of these molecules are quite distinct in size and degree of glycosylation.
Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina 27710.
M S
MS
Lan
S K
SK
Batra
W N
WN
Qi
R S
RS
Metzgar
M A
MA
Hollingsworth
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000235:genetics* / Q000302:isolation & purification; D015723:Gene Library; D006801:Humans; D007106:Immune Sera; D008969:Molecular Sequence Data; D009077:Mucins / Q000235:genetics*; D010190:Pancreatic Neoplasms / Q000235:genetics*; D014176:Protein Biosynthesis; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D012091:Repetitive Sequences, Nucleic Acid
D004273:DNA, Neoplasm; D007106:Immune Sera; D009077:Mucins; D012334:RNA, Neoplasm