Expression of cell-cycle-dependent genes in phytohemagglutinin-stimulated human lymphocytes.
We have investigated the expression of certain cell-cycle-dependent genes in human peripheral blood mononuclear cells (PBMC) stimulated by phytohemagglutinin (PHA). The genes studied had been previously identified as cell-cycle dependent in other cell types from different species and were induced by different mitogens. One of these genes (2F1) and the gene for the interleukin 2 receptor were induced by PHA even in cultures partially depleted of accessory cells where the lymphocytes grew in size but failed to enter S phase. The other genes (c-myc, 4F1, JE-3, and KC-1) were induced only in complete cultures of PBMC stimulated by PHA. These results confirm the dissociation between growth in size and cell DNA replication that can occur during cell-cycle progression. Moreover, the time course of appearance of detectable levels of RNA for these genes suggests that they may be used as markers of cell-cycle progression in the transition of lymphocytes from G0 to S phase.
L
L
Kaczmarek
B
B
Calabretta
R
R
Baserga
D002453:Cell Cycle; D005796:Genes; D006801:Humans; D007700:Kinetics; D008213:Lymphocyte Activation; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D010835:Phytohemagglutinins; D010957:Plasmids; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D014158:Transcription, Genetic
D010835:Phytohemagglutinins; D012313:RNA