[Principles of selective inactivation of the virus genome. IV. The effect of UV-irradiation of phage MS2 on its binding with anti-MS2-immunoglobulins].
Ultraviolet (254 nm) irradiation of the bacteriophage MS2 results in the decrease of the number of antigenic determinants exposed on the virion surface. The cross-section of the decrease, as measured by the number of anti-MS2 IgG molecules bound per virion, is 10(-16) mm2 per photon. The decrease of the phage-antibody binding proceeds after irradiation with a rate constant of about 5 x 10(-3) min-1. Since the antigenic determinants of the phage MS2 coat protein does not contain photoreactive amino acid residues, the irradiation-induced decrease of the phage antibody binding is determined, most probably, by the shielding of the antigenic determinants. Such shielding could be caused by rearrangement of coat protein molecules and/or of the capsid induced by photomodification of non-antigenic fragments of coat protein and/or of intraphage RNA.
E I
EI
Budovskiĭ
G V
GV
Kostiuk
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology* / Q000528:radiation effects; D001435:Bacteriophages / Q000235:genetics / Q000276:immunology* / Q000528:radiation effects; D002850:Chromatography, Gel; D000939:Epitopes / Q000276:immunology / Q000528:radiation effects; D005814:Genes, Viral; D007074:Immunoglobulin G / Q000276:immunology*; D011817:Rabbits; D014466:Ultraviolet Rays; D014759:Viral Envelope Proteins / Q000276:immunology / Q000528:radiation effects; D014771:Virion / Q000276:immunology / Q000528:radiation effects
D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D007074:Immunoglobulin G; D014759:Viral Envelope Proteins
Printsipy selektivnoĭ inaktivatsii virusnogo genoma. IV. Vliianie UF-oblucheniia faga MS2 na ego sviazyvanie s anti-MS2-immunoglobulinami.