In vitro translation of chicken type X collagen in the presence of pancreas microsomes.
Total RNA from epiphysis of 17-day-old chick embryo tibiae was used to direct protein synthesis in a wheat germ cell free system. The type X collagen chain, identified on the basis of its electrophoretic migration and of peptides obtained by S. aureus V8 protease digestion, was the major translation product. The newly synthesized chain included a signal sequence that was removed when dog pancreas membranes were added at the time of the protein synthesis.
P
P
Castagnola
F
F
Descalzi Cancedda
R
R
Cancedda
D000818:Animals; D002474:Cell-Free System; D002642:Chick Embryo; D003094:Collagen / Q000235:genetics*; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism; D004838:Epiphyses / Q000032:analysis; D008861:Microsomes / Q000378:metabolism*; D010179:Pancreas / Q000648:ultrastructure*; D014176:Protein Biosynthesis; D012313:RNA / Q000032:analysis; D012697:Serine Endopeptidases
D012313:RNA; D003094:Collagen; D010450:Endopeptidases; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase