A monoclonal antibody specific for snRNPs U1 and U2.
A monoclonal antibody (D-5) is described which selectively precipitates snRNPs U1 and U2. The antibody was derived from a mouse immunized with extracts from chick embryonic nuclei. By immunoblotting on either total proteins from purified snRNPs U1-U6, U2-U6 or U1 only, we could demonstrate that the monoclonal antibody cross-reacts with the U1 RNP specific polypeptide A and the U2 RNP specific polypeptide B", thereby establishing that the two snRNP proteins share at least one epitope. D-5 precipitates snRNPs U1 and U2 from a variety of species, including man, chicken, mouse, rat kangaroo and Xenopus laevis. It will thus be a useful tool for studying structure function relationships of the two snRNP species in different cell systems.
Reuter
R
R
Lehner
C F
CF
Nigg
E A
EA
Lührmann
R
R
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001667:Binding, Competitive; D002467:Cell Nucleus / Q000276:immunology; D002642:Chick Embryo; D004173:Dipodomys; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D006367:HeLa Cells / Q000032:analysis; D006801:Humans; D007158:Immunologic Techniques; D007163:Immunosorbent Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D012261:Ribonucleoproteins / Q000276:immunology*; D017411:Ribonucleoproteins, Small Nuclear; D014982:Xenopus laevis
D000911:Antibodies, Monoclonal; D000939:Epitopes; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear