Effect of hypothyroidism on pituitary cytoplasmic concentrations of messenger RNA encoding thyrotrophin beta and alpha subunits, prolactin and growth hormone.
Thyroid hormones may directly regulate gene expression in the anterior pituitary. In order to examine this possibility we have studied the effect of hypothyroidism in the rat on pituitary cytoplasmic concentrations of messenger RNA (mRNA) encoding thyrotrophin (TSH) beta and alpha subunits, prolactin and GH. We demonstrated a marked increase in TSH beta and alpha subunit mRNA, accompanied by a decrease in GH mRNA, in the hypothyroid state, changes largely reversed by thyroid hormone replacement. We have thus shown a direct influence of thyroid status on the pretranslational events occurring in pituitary hormone synthesis. The simultaneous rise in cytoplasmic TSH beta and alpha mRNA levels and fall in GH mRNA in hypothyroidism suggests that thyroid status exerts a differential effect on the expression of these genes.
J A
JA
Franklyn
T
T
Lynam
K
K
Docherty
D B
DB
Ramsden
M C
MC
Sheppard
D000818:Animals; D003593:Cytoplasm / Q000032:analysis; D005260:Female; D005786:Gene Expression Regulation; D015292:Glycoprotein Hormones, alpha Subunit; D013006:Growth Hormone / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010446:Peptide Fragments / Q000235:genetics; D010903:Pituitary Gland, Anterior / Q000032:analysis*; D010908:Pituitary Hormones, Anterior / Q000235:genetics; D011388:Prolactin / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013963:Thyroid Hormones / Q000235:genetics*; D013972:Thyrotropin / Q000235:genetics
D015292:Glycoprotein Hormones, alpha Subunit; D010446:Peptide Fragments; D010908:Pituitary Hormones, Anterior; D012333:RNA, Messenger; D013963:Thyroid Hormones; D011388:Prolactin; D013972:Thyrotropin; D013006:Growth Hormone