Neonatal human foreskin keratinocytes produce 1,25-dihydroxyvitamin D3.
Primary cultures of neonatal human foreskin keratinocytes converted 25-hydroxyvitamin D in high yield to a metabolite with the chromatographic behavior of 1,25-dihydroxyvitamin D3. The identity of this metabolite as 1,25-dihydroxyvitamin D3 was confirmed both by its potency in displacing 1,25-dihydroxyvitamin D3 in the chick cytosol receptor assay and by mass spectral analysis. These results suggest that 1,25-dihydroxyvitamin D3 may be formed in the epidermis to regulate vitamin D production by the epidermis and to provide an alternative to 1,25-dihydroxyvitamin D3 production by the kidneys.
Bikle
D D
DD
Nemanic
M K
MK
Whitney
J O
JO
Elias
P W
PW
D000818:Animals; D002112:Calcifediol / Q000378:metabolism; D002117:Calcitriol / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D002478:Cells, Cultured; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003600:Cytosol / Q000378:metabolism; D004817:Epidermis / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn; D007413:Intestinal Mucosa / Q000378:metabolism; D007633:Keratins / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D018167:Receptors, Calcitriol; D011987:Receptors, Steroid / Q000378:metabolism; D012867:Skin / Q000378:metabolism*; D014316:Tritium
D018167:Receptors, Calcitriol; D011987:Receptors, Steroid; D014316:Tritium; D007633:Keratins; D002117:Calcitriol; D002112:Calcifediol