Adjuvant actions of linear mannan-possessing lipopolysaccharide (LPS) in LPS-resistant C3H/HeJ mice.
Immunopotentiation has been demonstrated when Klebsiella O3 lipopolysaccharide (KO3 LPS), which possesses a linear mannan as the O-specific side chain, was injected subcutaneously into endotoxin resistant C3H/HeJ mice together with soluble protein antigens. The LPS exhibited significantly positive adjuvant effects on antibody responses in vivo after secondary antigen challenge and on delayed-type hypersensitivity (DTH) reactions against protein antigens. However, KO3 LPS was not a polyclonal B cell activator (PBA) in C3H/HeJ mice nor mitogenic in cultures of spleen cells of C3H/HeJ. Thus, the activity of the LPS in C3H/HeJ mice is confined to the potentiation of T-dependent immune responses. The contribution of the mannan O side chain to the adjuvant action of KO3 LPS was suggested.
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Ohta
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Kido
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Nakashima
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Kato
D000276:Adjuvants, Immunologic; D000818:Animals; D000917:Antibody Formation; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007709:Klebsiella; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D008934:Mitogens; D013154:Spleen / Q000276:immunology; D000511:alpha-Macroglobulins / Q000276:immunology
D000276:Adjuvants, Immunologic; D008070:Lipopolysaccharides; D008934:Mitogens; D000511:alpha-Macroglobulins; C032306:polyclonal B cell activator