Vascular permeability to macromolecules changes qualitatively in inflammation?
The time courses of vascular permeability to native bovine serum albumin (BSA), cationized BSA, dextran (mol wt. 40,000) and bovine immunoglobulin G (IgG) in carrageenin-induced inflammation in rats were determined by the fluorometric method. The vascular permeability to BSA increased gradually until about 5 hr after carrageenin injection. The vascular permeabilities to dextran and IgG reached a maximum at 1 hr after carrageenin injection and then decreased. In the early-stage, 0-1 hr after carrageenin injection, dextran was the most permeating of the three. However, in the later-stage, 3-5 hr after carrageenin injection, BSA became the most permeating. Furthermore, cationized BSA was more permeating than native BSA having a negative charge in the early-stage, but the difference between the permeability to cationized BSA and native BSA was decreased at the later-stage. These data suggest that vascular permeability changes qualitatively in carrageenin-induced inflammation in rats, and it is unlikely that the increased vascular permeability is caused by the ultrafiltration through gaps formed between endothelial cells.
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D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D002199:Capillary Permeability; D002417:Cattle; D003911:Dextrans / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D007249:Inflammation / Q000503:physiopathology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D001798:Blood Proteins; D003911:Dextrans; D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine