Colorimetric assay of gramicidin A in the presence of surfactants and phospholipids.
Gramicidin A, a hydrophobic polypeptide containing 4 tryptophan residues/molecule, may be determined quantitatively after reaction with 4-(dimethylamino)benzaldehyde, a method previously proposed for tryptophan analysis. The assay may be carried out even in the presence of various surfactants and phospholipids at high concentrations.
M A
MA
Urbaneja
A
A
Alonso
F M
FM
Goñi
D001547:Benzaldehydes; D003124:Colorimetry; D006096:Gramicidin / Q000032:analysis*; D017830:Octoxynol; D010743:Phospholipids; D011092:Polyethylene Glycols; D013501:Surface-Active Agents; D014364:Tryptophan / Q000032:analysis
D001547:Benzaldehydes; D010743:Phospholipids; D013501:Surface-Active Agents; D006096:Gramicidin; D011092:Polyethylene Glycols; D014364:Tryptophan; D017830:Octoxynol; C510241:p-dimethylaminobenzaldehyde