Expression in bacteria of gB-glycoprotein-coding sequences of Herpes simplex virus type 2.
A plasmid with an insert that encodes the glycoprotein B(gB) gene of Herpes simplex virus type 2 (HSV-2) has been isolated. DNA sequences coding for a portion of the HSV-2 gB peptide were cloned into a bacterial lacZ alpha expression vector and used to transform Escherichia coli. Upon induction of lacZpo-promoted transcription, some of the bacteria became filamentous and produced inclusion bodies containing a large amount of a 65-kDal peptide that was shown to be precipitated by broad-spectrum antibodies to HSV-2 and HSV-1. The HSV-2 insert of one of these clones specifies amino acid residues corresponding to 135 through 629 of the gB of HSV-1 [Bzik et al., Virology 133 (1984) 301-314].
Person
S
S
Warner
S C
SC
Bzik
D J
DJ
Debroy
C
C
Fox
B A
BA
D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000235:genetics*; D003001:Cloning, Molecular; D000939:Epitopes; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D006023:Glycoproteins / Q000235:genetics; D008970:Molecular Weight; D018139:Simplexvirus / Q000235:genetics* / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics*; D014765:Viral Vaccines / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D006023:Glycoproteins; D014764:Viral Proteins; D014765:Viral Vaccines