Immunofluorescent flow cytometry in N dimensions. The multiplex labeling approach.
The problem of the simultaneous use in flow cytometry of N greater than 2 antibodies in conjunction with two fluorochromes was investigated. Theoretical analysis led to a labeling procedure and reconstruction formula that allow N-dimensional labeling distributions to be obtained from two-dimensional fluorescence distributions. The general problem of M greater than or equal to 2 fluorochromes and N greater than M antibodies was shown to be reducible to the case of two fluorochromes. The method was tested by a triple labeling analysis of murine thymocytes.
T N
TN
Buican
G W
GW
Hoffmann
D000818:Animals; D000911:Antibodies, Monoclonal; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D005455:Fluorescent Antibody Technique; D008433:Mathematics; D051379:Mice; D008808:Mice, Inbred CBA; D008954:Models, Biological; D013950:Thymus Gland / Q000166:cytology* / Q000276:immunology
D000911:Antibodies, Monoclonal