Alkaline phosphatase and 5'-nucleotide phosphodiesterase from bovine intestine are cross-reactive.
Polyclonal antibodies to native alkaline phosphatase and to native 5'-nucleotide phosphodiesterase were found to strongly cross-react with both enzymes. The antibodies also cross-react with both denatured enzymes, with glycopeptides from 5'-nucleotide phosphodiesterase, and with the oligosaccharides remaining after Pronase E digestion of the phosphodiesterase. They do not cross-react with either enzyme after their oligosaccharides have been modified or removed by periodate or trifluoromethanesulfonic acid treatment. Antibodies to denatured 5'-nucleotide phosphodiesterase do not bind to the native phosphodiesterase or alkaline phosphatase but do cross-react with denatured alkaline phosphatase even after removal or modification of the carbohydrate moieties. These results suggest that antibodies to denatured 5'-nucleotide phosphodiesterase may recognize amino acid sequence homology between alkaline phosphatase and 5'-nucleotide phosphodiesterase. However, antibodies to native enzymes apparently recognize cross-reactive determinants of the native enzymes which are carbohydrate in nature. This is the first report of antimammalian alkaline phosphatase antibodies which recognize the carbohydrate moieties of the enzyme.
J S
JS
Culp
L G
LG
Butler
D000469:Alkaline Phosphatase / Q000276:immunology*; D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D002241:Carbohydrates / Q000032:analysis; D002417:Cattle; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis; D005779:Immunodiffusion; D007422:Intestines / Q000201:enzymology*; D007700:Kinetics; D011755:Pyrophosphatases / Q000276:immunology*
D000906:Antibodies; D000936:Antigen-Antibody Complex; D002241:Carbohydrates; D000939:Epitopes; D000469:Alkaline Phosphatase; D011755:Pyrophosphatases; C021899:nucleotide pyrophosphatase