Determination of streptomycin in serum by high-performance liquid chromatography.
A high-performance liquid chromatographic method was developed for monitoring the serum concentration of streptomycin. The method includes clean-up using a Sep-Pak C18 cartridge and quantitation using dihydrostreptomycin as an internal standard. Streptomycin and dihydrostreptomycin were separated by reversed-phase ion-pair chromatography on LiChrosorb RP-18 and detected by UV absorption (195 nm). The calibration graph of serum streptomycin concentration was linear over the range 5-50 micrograms/ml. Streptomycin was added to serum at the level of 20.0 micrograms/ml and its concentration was determined to be 18.9 micrograms/ml with a coefficient of variation of 2.07% (n = 5). The clinical application of this method was confirmed by comparison with fluorescence polarization immunoassay.
N
N
Kurosawa
S
S
Kuribayashi
E
E
Owada
K
K
Ito
M
M
Nioka
M
M
Arakawa
R
R
Fukuda
D002851:Chromatography, High Pressure Liquid; D004096:Dihydrostreptomycin Sulfate / Q000097:blood; D005454:Fluorescence Polarization; D005455:Fluorescent Antibody Technique; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007700:Kinetics; D013307:Streptomycin / Q000097:blood*
D007202:Indicators and Reagents; D004096:Dihydrostreptomycin Sulfate; D013307:Streptomycin