Presence of immunoreactive endothelin in human milk.
Endothelin-like immunoreactivity was detected in human milk at a concentration of 6.8 +/- 1.6 pmol/l (mean +/- SEM; n = 16) using a highly sensitive radioimmunoassay. Gel filtration and fast protein liquid chromatography (FPLC) verified the identity of the endothelin. FPLC revealed 4 peaks, one eluting just after the void volume, and the other three in the positions of endothelin-1, -2, and -3, respectively.
Lam
H C
HC
Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, England.
Takahashi
K
K
Ghatei
M A
MA
Bloom
S R
SR
D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D016232:Endothelins; D004730:Endothelium, Vascular; D005260:Female; D006801:Humans; D008895:Milk, Human / Q000032:analysis*; D010455:Peptides / Q000032:analysis*; D011863:Radioimmunoassay; D012016:Reference Values
D016232:Endothelins; D010455:Peptides