Identification of spinothalamic tract cells in fresh, unfixed rat spinal cord.
Diamidino yellow dihydrochloride (DY . 2HCl) can reliably label spinothalamic tract cells both in the perfused, fixed and in the fresh, unfixed rat spinal cord. The major advantages of DY . 2HCl as a retrograde marker are that it leaks from the cells very slowly and can be conveniently identified in unfixed neurons by fluorescence microscopy. DY . 2HCl will be a useful marker for identification of spinothalamic tract cells that are to be studied in vitro.
Huang
L Y
LY
Carlton
S M
SM
Willis
W D
WD
D000578:Amidines; D000818:Animals; D008856:Microscopy, Fluorescence; D051381:Rats; D011919:Rats, Inbred Strains; D013133:Spinothalamic Tracts / Q000033:anatomy & histology* / Q000166:cytology; D013194:Staining and Labeling / Q000379:methods
D000578:Amidines; C037945:diamidino yellow