High-performance liquid chromatographic determination of 4-amino-5-chloro-N-[(1-ethylimidazolin-2-yl)methyl]-2-methoxybenzamide and its metabolite in biological fluids. Applications in pharmacokinetic studies.
A high-performance liquid chromatographic method with spectrofluorometric detection has been developed for the analysis of a new substituted benzamide and its metabolite in body fluids. The detection was performed at 270 nm for excitation, and 370 nm for emission. The proposed technique is selective, reliable, and sensitive. The detection limit is 1 ng/mL for the parent drug and 2 ng/mL for the imidazole derivative. The reproducibility of the analytical method through statistical coefficients is approximately 5%. The accuracy of the method is good; that is, the relative error is less than 2%. To validate this method, the stability of these two drugs was tested at the time of conservation of urine and plasma samples and during all steps of the analytical method.
Groupe de Recherche en Pharmacocinétique, Laboratoire de Chimie Analytique, Faculté de Pharmacie, Montpellier, France.
F
F
Bressolle
J
J
Bres
A
A
Moulin
D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D004355:Drug Stability; D005764:Gastroesophageal Reflux / Q000188:drug therapy*; D006207:Half-Life; D006801:Humans; D007093:Imidazoles / Q000032:analysis* / Q000493:pharmacokinetics; D007275:Injections, Intravenous; D008253:Macaca mulatta; D051381:Rats; D013045:Species Specificity
D007093:Imidazoles; C065607:lintopride