The role of RNA molecules in transduction of the proto-oncogene c-fps.
Transduction of cellular genes by retroviruses requires two recombinations: one to form the left-hand junction between cellular gene and viral genome, the other to form the right-hand junction. Previous findings raised the possibility that the right-hand recombination might use RNA molecules as intermediates. We now provide direct experimental support for this view by showing that the right-hand end of v-fps in the avian sarcoma virus PRCII was formed by recombination within the poly(A) tract at the 3' end of the mRNA for the proto-oncogene c-fps. Recombination of this sort may be mediated by "copy-choice" during reverse transcription, acting on either homologous or non-homologous nucleotide sequences.
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CC
Huang
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Hay
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JM
Bishop
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D004247:DNA / Q000032:analysis; D005814:Genes, Viral; D005822:Genetic Vectors; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000378:metabolism / Q000502:physiology; D011519:Proto-Oncogenes; D012313:RNA / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism / Q000502:physiology; D011995:Recombination, Genetic; D012190:Retroviridae / Q000235:genetics; D014161:Transduction, Genetic
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA