Fetal liver transplantation in the dog. II. Repopulation of the granulocyte-macrophage progenitor cell compartment by fetal liver cells from DLA-identical siblings.
The restoration of the granulocyte-macrophage progenitor cell (CFU-GM) compartments in blood and bone marrow, and the recovery of blood monocytes were followed for up to one year in ten beagles that had been exposed to fractionated (3 X 6 Gy) total-body irradiation before being transfused with cryopreserved fetal liver cells (FLC) from sibling donors that were genotypically matched for dog leukocyte antigens. Grafts contained 0.2-1.6 X 10(8) mononuclear cells and 0.9-19.8 X 10(4) CFU-GM/kg body weight. Numbers of circulating monocytes rose parallel to granulocyte numbers after day 6 and became normal by day 18 posttransplant. In bone marrow aspirates, low numbers of CFU-GM were detected on day 3 and their incidence per 10(5) mononuclear cells was normal after day 14. Circulating CFU-GM were present in significant numbers by day 7 and their elevated concentration per milliliter of blood after day 14 continued for one year. Dextran sulfate injection mobilized normal numbers of CFU-GM into the blood early after transplantation, and spontaneously circulating CFU-GM in a later phase did not differ from blood progenitors of normal animals with respect to radiation sensitivity and sedimentation velocity. Thus, FLC transplantation effected a rapid restoration of granulopoiesis and monocytopoiesis, which was reflected at both the level of mature blood cells and the compartments of CFU-GM in blood and bone marrow, underlining the high repopulating capacity of fetal liver stem cells.
Prümmer
O
O
Werner
C
C
Raghavachar
A
A
Nothdurft
W
W
Calvo
W
W
Steinbach
K H
KH
Fliedner
T M
TM
D000818:Animals; D001772:Blood Cell Count; D001831:Body Temperature; D001854:Bone Marrow Cells; D003115:Colony-Stimulating Factors / Q000097:blood; D016264:Dextran Sulfate; D003911:Dextrans / Q000494:pharmacology; D004285:Dogs; D005260:Female; D005333:Fetus; D006098:Granulocytes / Q000166:cytology; D006410:Hematopoiesis; D016031:Liver Transplantation; D008264:Macrophages / Q000166:cytology; D008297:Male; D009000:Monocytes / Q000166:cytology; D013234:Stem Cells
D003115:Colony-Stimulating Factors; D003911:Dextrans; D016264:Dextran Sulfate