5-Hydroxyindoleacetic acid and 5-hydroxytryptophol levels in rat brain: effects of ethanol, pyrazole, cyanamide and disulfiram treatment.
The two serotonin metabolites 5-hydroxyindoleacetic acid (5HIAA) and 5-hydroxytryptophol (5HTOL) were measured in two regions of rat brain (pons medulla and diencephalon) using a gas chromatographic-mass spectrometric (GC-MS) method. Acute ethanol intoxication effected an elevation of 5-hydroxytryptophol levels, while 1 week of treatment with ethanol appeared to have no effect on either metabolite when measured 24 h after the last dose. Disulfiram and cyanamide treatment produced an approximately 2-fold increase in 5-hydroxytryptophol and a slight reduction in 5-hydroxyindole-acetic acid. Pyrazole treatment produced an increase in both metabolites. This effect was, however, counteracted by the simultaneous administration of ethanol.
O
O
Beck
C J
CJ
Eriksson
K
K
Kiianmaa
A
A
Lundman
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D003484:Cyanamide / Q000494:pharmacology*; D003486:Cyanides / Q000494:pharmacology*; D004027:Diencephalon / Q000187:drug effects; D004221:Disulfiram / Q000494:pharmacology*; D000431:Ethanol / Q000494:pharmacology*; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism*; D006917:Hydroxytryptophol / Q000378:metabolism*; D007211:Indoles / Q000378:metabolism*; D008297:Male; D008526:Medulla Oblongata / Q000187:drug effects; D011149:Pons / Q000187:drug effects; D011720:Pyrazoles / Q000494:pharmacology*; D051381:Rats; D012701:Serotonin / Q000378:metabolism
D003486:Cyanides; D007211:Indoles; D011720:Pyrazoles; D006917:Hydroxytryptophol; D012701:Serotonin; D000431:Ethanol; C031280:pyrazole; D003484:Cyanamide; D006897:Hydroxyindoleacetic Acid; D004221:Disulfiram