Induction of endogenous xenotropic retrovirus expression by the tumor promoter 12-O-tetradecanoylphorbol-13-acetate.
The tumor promotor 12-O-tetradecanoylphorbol-13-acetate (TPA) was examined for its ability to induce endogenous retrovirus from a high-passage clone of Kirsten sarcoma virus-transformed Balb/c (K-Balb) mouse cells. TPA activated virus in a concentration-dependent manner (0.0016 to 4.0 microM). Exposure to 1mM actinomycin D inhibited virus induction, suggesting that cellular RNA synthesis is required de novo by this inducer. A broad-spectrum neutralizing antibody to murine type C virus, gp70, was shown to neutralize the infectivity of the induced virus. The activated virus had the host range of the xenotropic Balb virus:2, and after removal of the inducer, the activated state decayed rapidly. TPA stimulated DNA, RNA, and protein synthesis in K-Balb cells, indicating that the mechanism of induction may be different from that of previously identified virus inducers. The effects observed using the well-defined K-Balb system offer an opportunity to study the modulation of retrovirus gene expression by TPA.
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D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D007708:Kirsten murine sarcoma virus / Q000502:physiology; D051379:Mice; D009500:Neutralization Tests; D010704:Phorbols / Q000494:pharmacology*; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D012190:Retroviridae / Q000254:growth & development* / Q000276:immunology; D012191:Retroviridae Proteins / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014775:Virus Activation / Q000187:drug effects*
D010704:Phorbols; D012191:Retroviridae Proteins; D012313:RNA; D004247:DNA; D013755:Tetradecanoylphorbol Acetate