Fibronectin distribution in normal and malignant urothelium.
Fibronectin is a glycoprotein that mediates the attachment of BCG to the murine bladder. To assess the potential role of fibronectin on bladder cancer cells as a specific substrate for BCG binding in man, a semi-quantitative method was employed to evaluate the presence of fibronectin on normal urothelium and bladder cancer. Monoclonal anti-fibronectin binding to normal and malignant urothelial tissues was evaluated by an immunoperoxidase assay. Human tumor cell lines were evaluated with mixed hemadsorption and immunoperoxidase assays. In both systems, immunoreactive fibronectin had low expression on unfixed normal and malignant urothelium. With fixation, immunoreactive fibronectin decreased on supporting stroma and increased in normal and malignant urothelium. Fibronectin distribution did not show tumor specificity either with fixed or unfixed specimens.
Department of Surgery, University of Michigan Medical Center, Ann Arbor 48109.
H B
HB
Grossman
M
M
Liebert
G
G
Wedemeyer
G
G
Wilson
A
A
Flint
D001500:BCG Vaccine / Q000627:therapeutic use; D001666:Binding Sites, Antibody; D004848:Epithelium / Q000032:analysis; D005353:Fibronectins / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000032:analysis* / Q000628:therapy
D001500:BCG Vaccine; D005353:Fibronectins