Monoclonal antibodies against the presynaptic calcium channel antagonist omega-conotoxin GVI A from cone snail poison.
Monoclonal antibodies have been prepared against omega-conotoxin GVI A, a peptide isolated from marine snails of the genus Conus (Conus geographus and Conus magus). This toxin is a blocker of select presynaptic Ca2+ channels in the central nervous system. Antigenic omega-conotoxin GVI A was synthesized as a covalent conjugate with bovine serum albumin and injected s.c. An ELISA assay combined with a competitive inhibition assay was used to select and characterize monoclonal antibodies able to recognize and bind the free toxin. Several of the antibodies were found to block omega-conotoxin GVI A inhibition of 45Ca transport into rat brain synaptosomes and to block omega-conotoxin GVI A binding to membranes from the same preparation. The antibodies recognize native, synthetic toxin, and are useful for analysis of toxin in biological fluids.
Tombaccini
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Laboratory of Cell Biology and Genetics, NIDDK, National Institutes of Health, Bethesda, MD.
Adeyemo
O M
OM
Pollard
H B
HB
Feuerstein
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G
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000494:pharmacology; D000941:Antigens / Q000276:immunology; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism; D002121:Calcium Channel Blockers; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006054:Goldfish; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D008978:Mollusk Venoms / Q000032:analysis / Q000276:immunology* / Q000378:metabolism; D051381:Rats; D012710:Serum Albumin, Bovine / Q000276:immunology; D013574:Synaptosomes / Q000378:metabolism; D020866:omega-Conotoxin GVIA
D000911:Antibodies, Monoclonal; D000941:Antigens; D002121:Calcium Channel Blockers; D008978:Mollusk Venoms; D012710:Serum Albumin, Bovine; D020866:omega-Conotoxin GVIA