alpha-L-fucosidase from aspergillus niger: demonstration of a novel alpha-L-(1----6)-fucosidase acting on glycopeptides.
An alpha-L-fucosidase which hydrolyzes fucose from alpha-(1----6)-linkage to N-acetylglucosamine was found in Aspergillus niger. The enzyme was purified by affinity chromatography with bovine IgG glycopeptide-Sepharose 4B. The enzyme preparation released fucose from bovine IgG glycopeptide and fucosylated asialoagalactofetuin, but failed to cleave 1----2, 1----3 or 1----4 linkages of alpha-L-fucosides.
Yazawa
S
S
Madiyalakan
R
R
Chawda
R P
RP
Matta
K L
KL
D000117:Acetylglucosamine / Q000378:metabolism; D001212:Asialoglycoproteins; D001234:Aspergillus niger / Q000201:enzymology*; D002846:Chromatography, Affinity; D060748:Fetuins; D005643:Fucose / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D006020:Glycopeptides / Q000378:metabolism*; D007700:Kinetics; D046911:Macromolecular Substances; D008970:Molecular Weight; D013379:Substrate Specificity; D000509:alpha-Fetoproteins / Q000378:metabolism; D005644:alpha-L-Fucosidase / Q000302:isolation & purification / Q000378:metabolism*
D001212:Asialoglycoproteins; D060748:Fetuins; D006020:Glycopeptides; D046911:Macromolecular Substances; D000509:alpha-Fetoproteins; C017029:asialofetuin; D005643:Fucose; D005644:alpha-L-Fucosidase; D000117:Acetylglucosamine; D005690:Galactose