Use of the IDEIA ELISA to detect Chlamydia psittaci (ovis) in material from aborted fetal membranes and milk from ewes affected by ovine enzootic abortion.
The IDEIA ELISA was used to detect Chlamydia psittaci (ovis) antigen in ewes' milk to which were added serial dilutions of chlamydiae titrated as inclusion forming units (ifus) in McCoy cell tissue culture. The test was able to detect as few as 35 ifus/ml of the organism. The ELISA was then used to detect chlamydial antigen in fetal membranes and milk from ewes clinically affected with ovine enzootic abortion (OEA). The results were compared with results of isolation of chlamydiae in McCoy cell tissue culture from the same material. The fetal membranes of 17 of 19 ewes were positive for chlamydia when tested with the ELISA but chlamydia could be cultured from only 15 of them. Milk samples from 26 ewes which had aborted between 1 and 34 days previously were tested: chlamydiae could not be cultured from any of them and only one was positive when tested by the ELISA. The results show that the IDEIA ELISA is a sensitive test for the detection of C. psittaci (ovis) antigens. The positive results to this test for the three samples from which chlamydiae could not be cultured suggest that the test is not as specific as culture or that it detected dead organisms. Chlamydiae do not appear to be excreted in the milk of ewes affected with OEA.
Thomas
R
R
Department of Veterinary Medicine and Animal Husbandry, Royal Veterinary College, Boltons Park, Potters Bar, Herts.
Davison
H C
HC
Wilsmore
A J
AJ
D000034:Abortion, Veterinary / Q000382:microbiology*; D000818:Animals; D002691:Chlamydophila psittaci / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D005321:Extraembryonic Membranes / Q000382:microbiology; D005260:Female; D008892:Milk / Q000382:microbiology; D011247:Pregnancy; D009956:Psittacosis / Q000382:microbiology / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*