Cloning and characterization of chloroplast ribosomal protein-encoding genes, rpl16 and rps3, of the marine macro-algae, Gracilaria tenuistipitata.
In Gracilaria tenuistipitata, a highly differentiated multicellular member of the marine red algae, Rhodophyta, chloroplast (cp) DNA can be separated as a satellite band from the nuclear DNA in a CsCl gradient. Using a heterologous probe from Chlamydomonas, the ribosomal protein-encoding gene, rpl16, was located on a 4.5-kb EcoRI fragment of cp DNA. The fragment was cloned and a 1365-bp region around rpl16 was sequenced. The gene order around rpl16, 5' rpl22-rps3-rpl16, is identical to that detected in the chloroplast DNA of liverwort, tobacco and maize. Both the nucleotide sequence and the amino-acid sequence of rpl16 are more conserved than that of rps3. The rpl16 gene contains no intron, a feature which shows more similarity to the unicellular green algae, Chlamydomonas, than to other land plants. Sequences that may form a stable stem-loop structure were detected within the coding sequence of rpl16.
Institute of Molecular Biology, Academia Sinica, Nankang, Taipei, Taiwan.
J S
JS
Kao
M
M
Wu
Y M
YM
Chiang
D000595:Amino Acid Sequence; D001483:Base Sequence; D002696:Chlamydomonas / Q000235:genetics; D002736:Chloroplasts; D003001:Cloning, Molecular; D004276:DNA, Satellite / Q000032:analysis; D015246:Deoxyribonuclease EcoRI; D005800:Genes, Fungal; D007438:Introns; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010944:Plants / Q000235:genetics*; D015183:Restriction Mapping; D000461:Rhodophyta / Q000235:genetics* / Q000254:growth & development; D012269:Ribosomal Proteins / Q000096:biosynthesis / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D004276:DNA, Satellite; D012269:Ribosomal Proteins; D015246:Deoxyribonuclease EcoRI