A sensitive and rapid enzyme-linked immunosorbent assay using monoclonal antibodies for simultaneous quantitation of free and IgA-complexed protein HC.
A sensitive and rapid enzyme-linked immunosorbent assay for simultaneous quantitation of free and IgA-complexed human protein HC was developed with monoclonal murine antibodies. The total amount of protein HC (free plus IgA-complexed) was measured by a competitive procedure while the protein HC-IgA complex was quantitated by a sandwich enzyme immunoassay. The amount of free protein HC was then obtained as the difference between the 2 measured values. The sensitivity of the procedure was 70 micrograms/1 for the total amount of protein HC and 80 micrograms/1 for the protein HC-IgA complex. At ordinary human serum levels of free protein HC and protein HC-IgA complexes the coefficient of variation for the procedure was about 5%. One worker could determine the concentrations of free and IgA-complexed protein HC in up to 400 serum samples in a working day.
J L
JL
Fernandez-Luna
I
I
Moneo
A
A
Grubb
E
E
Méndez
D000510:Alpha-Globulins / Q000032:analysis* / Q000097:blood / Q000276:immunology / Q000652:urine; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007070:Immunoglobulin A
D000510:Alpha-Globulins; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D007070:Immunoglobulin A; C016001:alpha-1-microglobulin