Effects of subunit cross-linking on the properties of crotoxin.
Crotoxin was cross-linked using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride. Cross-linked crotoxin had the expected amino-terminal amino acids, amino acid composition, behavior on SDS-PAGE and an 80% reduction of reactable lysine residues. It was also non-toxic, had reduced immunological cross-reactivity toward both poly- and monoclonal antibodies raised to the basic subunit of crotoxin and had lost greater than 95% of its phospholipase activity. Loss of toxicity is due to either subunit cross-linking or the modification of essential residues.
Department of Molecular Biology, University of Wyoming, Laramie 82071.
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Lennon
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Plummer
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Kaiser
D000596:Amino Acids / Q000032:analysis; D003432:Cross-Linking Reagents / Q000494:pharmacology; D003435:Crotalid Venoms / Q000633:toxicity*; D003439:Crotoxin / Q000032:analysis / Q000276:immunology / Q000633:toxicity*; D019811:Hydroxylamine; D006898:Hydroxylamines / Q000494:pharmacology
D000596:Amino Acids; D003432:Cross-Linking Reagents; D003435:Crotalid Venoms; D006898:Hydroxylamines; D019811:Hydroxylamine; D003439:Crotoxin