Antibody against T lymphoblastic leukemia-associated antigen (3-40) identifies vimentin and keratin intermediate filaments in normal cells.
Antibody 3-40 defines a 35- to 40-kd surface antigen present on T lymphoblastic leukemia (T-ALL) cells that is absent on normal hematopoietic cells (Naito et al, Blood 62:852, 1983). Using immunoblot analysis of cytoskeletal proteins and indirect immunofluorescence of cell lines treated with various cytoskeletal inhibitors, in this report we have demonstrated that antibody 3-40 also identifies vimentin intermediate filaments (IMF) within the HSB-2 (T-ALL) cell line as well as in normal thymocytes, peripheral blood mononuclear cells, and human and rodent fibroblast cell lines. Cross-reactivity with several keratin subclasses was demonstrated in both human and rodent epithelial cell lines, human thymus, and skin. In addition, we have shown that antibody 3-40 defined a 39-kd intracellular IMF-associated protein in HSB-2 cells, epithelial and fibroblast cell lines. This IMF-associated protein may be selectively expressed on the surface of human T cells during malignant transformation.
Laster
A J
AJ
Palker
T J
TJ
Harden
E A
EA
Ho
W
W
Naito
K
K
DuPont
B
B
Haynes
B F
BF
D000818:Animals; D000912:Antibodies, Neoplasm; D000937:Antigen-Antibody Reactions; D000951:Antigens, Neoplasm / Q000276:immunology*; D002460:Cell Line; D003599:Cytoskeleton / Q000276:immunology*; D004848:Epithelium / Q000276:immunology; D005347:Fibroblasts / Q000276:immunology; D006801:Humans; D007633:Keratins / Q000276:immunology; D051381:Rats; D013347:Subcellular Fractions / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014746:Vimentin / Q000276:immunology
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D014746:Vimentin; D007633:Keratins