[Control of melanogenesis by glycosylation inhibitors and the inhibitory effect of interferon on melanoma metastasis].
The Glycosylation inhibitors, glucosamine or tunicamycin induced a marked loss of pigment within melanoma cells in addition to their reduced metastatic ability. Electrophoresis of tyrosinase demonstrated the disappearance of or a marked decrease in membrane-bound tyrosinase, T3 in the small and large-granule fractions. Glycoprotein synthesis in the melanogenic subcellular compartments of pigment cells seems to play an integral role in melanogenesis which is principally enhanced in their carcinogenic status. The effect of interferon (IFN) on melanoma metastasis was investigated using B16-F10 melanoma cells. The inhibitory effect was maximal when given 3 h prior to tumor cell inoculation. IFN given 12 and 24 h prior to, as well as simultaneously with, tumor cell inoculation, also reduced metastases, but to a lesser extent. When given 2 h after the inoculation, no effect was shown. The salutary effect of IFN was abolished by anti-asialo GMI, but NK activity was enhanced equally throughout 3 to 24 hrs. This indicates that the effect is substantially dependent on NK cell activity, although the implication of other factors is not excluded.
Mishima
Y
Y
Ueda
M
M
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D005944:Glucosamine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D007372:Interferons / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000276:immunology; D008175:Lung Neoplasms / Q000473:pathology / Q000556:secondary*; D008543:Melanins / Q000096:biosynthesis; D008545:Melanoma / Q000276:immunology / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D014442:Monophenol Monooxygenase / Q000378:metabolism; D009368:Neoplasm Transplantation; D013347:Subcellular Fractions / Q000201:enzymology; D014415:Tunicamycin / Q000494:pharmacology*
D008543:Melanins; D014415:Tunicamycin; D007372:Interferons; D014442:Monophenol Monooxygenase; D005944:Glucosamine