Induction of albumin gene transcription in hepatocytes by extracellular matrix proteins.
Transcriptional activity of the albumin gene was induced in primary cultures of hepatocytes by adding dilute concentrations of basement membrane-like proteins derived from the EHS mouse sarcoma tumor to established type I collagen cultures. By immunofluorescence microscopy with antialbumin antibody, the population of cells responded uniformly to dilute EHS. Of the three major components of EHS, purified laminin was as effective as unfractionated EHS at inducing an increase in albumin mRNA levels and albumin secretion; type IV collagen and heparan sulfate proteoglycan were ineffective.
Caron
J M
JM
Liver Core Center, University of California, San Francisco 94143.
D000818:Animals; D011508:Chondroitin Sulfate Proteoglycans / Q000494:pharmacology; D003094:Collagen / Q000494:pharmacology; D005109:Extracellular Matrix / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate / Q000494:pharmacology; D066298:In Vitro Techniques; D007797:Laminin / Q000494:pharmacology; D008099:Liver / Q000502:physiology*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D012709:Serum Albumin / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
D011508:Chondroitin Sulfate Proteoglycans; D003094:Collagen; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D007797:Laminin; D012333:RNA, Messenger; D012709:Serum Albumin