Immunovirological studies on human respiratory syncytial virus structural proteins.
Immunovirological studies suggest that human respiratory syncytial virus may well be composed of five structural proteins as are other members of the Paramyxoviridae family: the two external membrane glycoproteins H (90 000) and Fo (F1, 49 000; F2, 20 000; disulfide linked), the internal membrane protein M (34 000), the nucleoprotein N (42 000), and a protein (78 000) designated P that could be the equivalent of the polymerase of the morbillivirus and paramyxovirus genus. Neutralizing monoclonal antibodies showed, by immunoprecipitation and immunoblotting, that the fusion protein carries neutralizing epitopes. One monoclonal antibody, which shows a high neutralizing titer, immunoblotted directly with the F1 fragment (49 000) of the fusion protein. Analysis in mice of the immunogenicity of the structural proteins separated on sodium dodecyl sulphate gels indicated that, under our conditions, only the fusion protein dimer Fo and its F1 fragment were capable of inducing neutralizing antibodies.
M
M
Trudel
F
F
Nadon
C
C
Séguin
S
S
Ghoubril
P
P
Payment
P
P
Trépanier
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000918:Antibody Specificity; D002213:Capsid / Q000032:analysis / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D006801:Humans; D007122:Immunoelectrophoresis; D007163:Immunosorbent Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D012136:Respiratory Syncytial Viruses / Q000032:analysis* / Q000276:immunology; D014758:Viral Core Proteins / Q000032:analysis / Q000276:immunology; D014759:Viral Envelope Proteins / Q000032:analysis / Q000276:immunology; D014760:Viral Fusion Proteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000939:Epitopes; D014758:Viral Core Proteins; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins