Stable expression of cytolytic activity by influenza virus-specific cytotoxic T lymphocytes.
Influenza-specific immune cytotoxic T lymphocyte (CTL) populations maintain a constant level of in vitro cytolytic activity. This is demonstrable with both heterogeneous populations of anti-viral CTL from immune donors and long-term CTL clones derived from primed CTL precursors. Cytolytic machinery is stably expressed by these CTL populations under a variety of in vitro cultivation conditions. This finding is in contrast to results with alloreactive CTL generated by stimulation of primed CTL precursors that lose cytolytic activity on a per cell basis with time after stimulation. The results indicate that virus-specific, cloned CTL that stably express cytolytic activity are representative of the heterogeneous populations from which they are derived and further suggest a qualitative difference in the regulation and expression of cytolytic machinery between heterogeneous populations of influenza-specific CTL and alloreactive CTL.
Andrew
M E
ME
Braciale
V L
VL
Henkel
T J
TJ
Hatch
J A
JA
Braciale
T J
TJ
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D002999:Clone Cells / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000276:immunology; D009980:Influenza A virus / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D051381:Rats; D013154:Spleen / Q000166:cytology; D013602:T-Lymphocytes, Cytotoxic / Q000145:classification / Q000276:immunology*
D000956:Antigens, Viral; D000939:Epitopes