DNA sequences of frameshift and other mutations induced by ICR-170 in yeast.
ICR-170-induced mutations in the CYC1 gene of the yeast Saccharomyces cerevisiae were investigated by genetic and DNA sequence analyses. Genetic analysis of 33 cyc1 mutations induced by ICR-170 and sequence analysis of eight representatives demonstrated that over one-third were frameshift mutations that occurred at one site corresponding to amino acid positions 29-30, whereas the remaining mutations were distributed more-or-less randomly, and a few of these were not frameshift mutations. The sequence results indicate that ICR-170 primarily induces G.C additions at sites containing monotonous runs of three G.C base pairs. However, some (Formula: see text) sites within the CYC1 gene were not mutated by ICR-170. Thus, ICR-170 is a relatively specific mutagen that preferentially acts on certain sites with monotonous runs of G.C base pairs.
Ernst
J F
JF
Hampsey
D M
DM
Sherman
F
F
D000595:Amino Acid Sequence; D000609:Aminoacridines; D001483:Base Sequence; D003574:Cytochrome c Group / Q000235:genetics*; D045304:Cytochromes c; D004271:DNA, Fungal / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D009154:Mutation; D009588:Nitrogen Mustard Compounds / Q000494:pharmacology*; D009690:Nucleic Acid Conformation; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins
D000609:Aminoacridines; C014059:CYC1 protein, S cerevisiae; D003574:Cytochrome c Group; D004271:DNA, Fungal; D009588:Nitrogen Mustard Compounds; D029701:Saccharomyces cerevisiae Proteins; C091910:acridine mustard; D045304:Cytochromes c