Basally located epithelial cell surface component identified by a novel monoclonal antibody technique.
Tubular aggregates of glandular epithelial cells (gland fragments) were isolated from human endometrium by collagenase digestion of surrounding stroma, thus exposing the basal surfaces of the cells. Using these aggregates as immunogen, monoclonal antibodies could be derived that recognized basally located antigens. One such antibody, G71, is described, that binds to a basal epithelial cell antigen present in a variety of human epithelia. Epitope-bearing molecules in the range Mr 60 000-180 000 are present in two of the tissues studied, amnion and endometrium. The epitope is associated with areas of epithelial cell-extracellular matrix contact.
Aplin
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D000650:Amnion / Q000166:cytology; D000818:Animals; D000911:Antibodies, Monoclonal; D004591:Electrophoresis, Polyacrylamide Gel; D004717:Endometrium / Q000166:cytology*; D004847:Epithelial Cells; D000939:Epitopes / Q000032:analysis; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007163:Immunosorbent Techniques; D008597:Menstrual Cycle; D051379:Mice; D008807:Mice, Inbred BALB C; D003012:Microbial Collagenase / Q000378:metabolism; D008858:Microscopy, Phase-Contrast; D008970:Molecular Weight; D013499:Surface Properties
D000911:Antibodies, Monoclonal; D000939:Epitopes; D003012:Microbial Collagenase