Induction of IgG1 and IgE responses to protein-conjugated and unconjugated beta-lactam antibiotics in the mouse--efficacy of Freund's complete adjuvant.
One or two injections two weeks apart of protein-conjugated penicillin G, cephalothin or cefmetazole emulsified with Freund's complete adjuvant were quite effective in producing anti-antibiotic antibodies of the IgE as well as of the IgG1 class in mice. Long-lasting and boostable production of both antibody classes was also obtained against unconjugated cephalothin or cefmetazole, though the positivity depended on the mouse strain.
Harada
M
M
Watanabe
A
A
Matsumoto
M
M
Takeuchi
M
M
D000818:Animals; D000900:Anti-Bacterial Agents / Q000276:immunology*; D002417:Cattle; D005620:Freund's Adjuvant / Q000494:pharmacology*; D006168:Guinea Pigs; D006241:Haptens / Q000276:immunology; D007114:Immunization; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis*; D051379:Mice; D008809:Mice, Inbred C3H / Q000276:immunology; D008810:Mice, Inbred C57BL / Q000276:immunology; D012709:Serum Albumin / Q000276:immunology; D047090:beta-Lactams; D005719:gamma-Globulins / Q000276:immunology
D000900:Anti-Bacterial Agents; D006241:Haptens; D007074:Immunoglobulin G; D012709:Serum Albumin; D047090:beta-Lactams; D005719:gamma-Globulins; D007073:Immunoglobulin E; D005620:Freund's Adjuvant