A monoclonal antibody specific for the duplex DNA poly[d(TC)].poly[d(GA)].
Although most duplex DNAs are not immunogenic some synthetic DNAs such as poly[d(Tm5C)].poly[d(GA)] are weakly immunogenic allowing the production of monoclonal antibodies. The specificity of one of these antibodies, Jel 172, was investigated in detail by a competitive solid-phase radioimmune assay. Jel 172 bound well to poly[d(TC)].poly[d(GA)] but not to other duplex DNAs such as poly[d(TTC)].poly[d(GAA)] and poly[d(TCC)].poly[d(GGA)]. The binding to poly[d(Br5UC)].poly[d(GA)] was enhanced while that to poly[d(TC)].poly[d(IA)] was decreased compared to poly[d(TC)].poly[D(GA)]. Thus, not only is the antibody very specific for a sequence of duplex DNA but it also appears to recognize functional groups in both grooves of the helix.
Lee
J S
JS
Latimer
L J
LJ
Woodsworth
M L
ML
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D004247:DNA / Q000276:immunology*; D000939:Epitopes; D051379:Mice; D008810:Mice, Inbred C57BL; D011089:Polydeoxyribonucleotides / Q000276:immunology*; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D000939:Epitopes; D011089:Polydeoxyribonucleotides; C046661:poly(d(TC)).poly(d(GA)); D004247:DNA