[Method of automatic recording and analysis of leukocyte migration based on the image analysis system].
The method of automatic registration and of the analysis of neutrophils' mobility with the use of the Image Analysis System is described in this paper. The authors developed an algorithm of cells identification which makes it possible to carry out cadre-by-cadre observing the shift of each cell's centre of gravity with the time interval between cadres more than 15 seconds. This algorithm also allows to measure areas, perimeters and orientation of cells and their changes. Looking after the shifts of the cells' centres of gravity allows to describe the cells' paths in the form of the following dependences: path-by-time, shift-by-time, angle of vector of shift alteration. Observing a great number of cells at the same time allows to get true statistical picture of neutrophils' activity and distribution of cells' activity in population of neutrophils during only some minutes of registration. It is shown that each cell in the population has its own constant velocity.
Tumanov
E A
EA
Galkin
A A
AA
Filippov
M M
MM
Sakovich
V V
VV
Karelin
A A
AA
D000465:Algorithms; D002465:Cell Movement; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007962:Leukocytes / Q000166:cytology*; D009504:Neutrophils / Q000166:cytology
Metod avtomaticheskoĭ registratsii i analiza migratsii leĭkotsitov na baze sistemy obrabotki izobrazheniĭ.