Changes in RNA titers and polyadenylation during oogenesis and oocyte maturation in Xenopus laevis.
The titers of over 90 sequences isolated by cDNA cloning of oocyte poly(A)+RNA were examined during oogenesis in Xenopus laevis. The relative titers of most sequences in unfertilized eggs are established in pre-lamp brush oocytes and persist throughout oogenesis. We have identified several sequences whose titers decrease significantly during the growth phase of oogenesis as well as a few sequences whose titers increase slightly during this period. Among 21 sequences analyzed by RNA gel blots, all remained unchanged in titer during oocyte maturation. A significant fraction of early oocyte RNA does not bind to oligo(dT)cellulose, but by the end of oogenesis transcripts for many RNA species examined are detected exclusively in the poly(A)+RNA fraction. During oocyte maturation a slight size shift or a broadening of the hybridizing band can be seen for many sequences, indicative of poly(A) elongation or degradation.
Dworkin
M B
MB
Dworkin-Rastl
E
E
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000096:biosynthesis; D005260:Female; D009693:Nucleic Acid Hybridization; D009865:Oocytes / Q000254:growth & development* / Q000378:metabolism; D009866:Oogenesis; D011061:Poly A / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*; D012333:RNA, Messenger; D014158:Transcription, Genetic; D014982:Xenopus laevis
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA