Basophil histamine release induced by a substance from stimulated human platelets.
Platelet activation may occur during immunoglobulin E antibody (IgE)-mediated reactions. In these studies, we confirm that platelet-derived supernatants (PDS) induce histamine release from human mixed leukocytes containing basophils, one of the initial target cells in IgE-mediated reactions. In extending this observation, we have shown that this PDS-induced histamine release is both temperature- and calcium-dependent. Kinetic studies of release induced by PDS indicate that release is more rapid than that associated with IgE-dependent mechanisms. This platelet-derived, histamine-releasing activity is produced by platelet stimulation with collagen (5 micrograms/ml) and acetylglyceryl ether phosphorylcholine (10(-7)), as well as thrombin (1 U/ml). Initial characterization has shown that it is stable to acid and to freeze-thawing but not to boiling for 10 min. In addition, although this histamine-releasing activity is nondialyzable (i.e., greater than 3500 m.w.), it cannot be attributed to platelet factor 4. Thus, platelets, once activated, can produce a soluble substance or substances which can initiate basophil-mediated reactions, further suggesting that platelet activation can enhance allergic and inflammatory reactions.
M A
MA
Orchard
A
A
Kagey-Sobotka
D
D
Proud
L M
LM
Lichtenstein
D000941:Antigens / Q000276:immunology; D001491:Basophils / Q000502:physiology*; D001792:Blood Platelets / Q000502:physiology*; D003094:Collagen / Q000494:pharmacology; D006636:Histamine Release; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D007700:Kinetics; D010972:Platelet Activating Factor / Q000494:pharmacology; D013917:Thrombin / Q000494:pharmacology
D000941:Antigens; D010972:Platelet Activating Factor; D007073:Immunoglobulin E; D003094:Collagen; D013917:Thrombin