Determination of pentamidine in whole blood, plasma, and urine by high-performance liquid chromatography.
The analysis of pentamidine in whole blood, plasma, and urine by liquid chromatography is described. Extraction was made with a mixture of acetonitrile and chloroform followed by back-extraction into phosphate buffer. A reversed-phase chromatographic system with fluorescence detection was used. The precision of the method was 5-7% at the lower limit of determination (16 nmol/L in plasma and hemolyzed whole blood, 27.7 nmol/L in urine).
Ericsson
O
O
Department of Clinical Pharmacology, Huddinge University Hospital, Sweden.
Rais
M
M
D000097:Acetonitriles; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002725:Chloroform; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D005453:Fluorescence; D006801:Humans; D010419:Pentamidine / Q000032:analysis* / Q000097:blood / Q000652:urine; D012997:Solvents
D000097:Acetonitriles; D012997:Solvents; D010419:Pentamidine; D002725:Chloroform; C032159:acetonitrile