Steroid interactions with plasma membrane of decidual endometrium of the rat.
Plasma membranes were purified from deciduoma of pseudopregnant rats, rat liver and intestine, and calf uterus. Steroid binding evaluated with deciduoma plasma membranes showed competitive progestin binding, in contrast with estradiol binding which was nondisplaceable as measured by competition binding assay. When the photosensitive steroid [3H]-R5020 was photocrosslinked to plasma membrane, binding was reduced competitively by either progesterone or R5020. These results indicate that the decidual cell plasma membrane contains specific sites for interactions with progestins.
Department of Biology, State University of New York College at Fredonia 14063.
W N
WN
Yunghans
R N
RN
Day
S C
SC
Schiavi
T J
TJ
Desmond
D J
DJ
Morré
M W
MW
Bruner
D000818:Animals; D001667:Binding, Competitive; D002458:Cell Fractionation; D002462:Cell Membrane / Q000378:metabolism; D003656:Decidua / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism* / Q000648:ultrastructure; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D007413:Intestinal Mucosa / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D009649:Norpregnadienes / Q000378:metabolism*; D010777:Photochemistry; D011374:Progesterone / Q000378:metabolism*; D011397:Promegestone / Q000378:metabolism*; D011555:Pseudopregnancy; D051381:Rats; D014599:Uterus / Q000378:metabolism
D009649:Norpregnadienes; D011374:Progesterone; D004958:Estradiol; D011397:Promegestone