The detection of oligoclonal IgG bands in unconcentrated cerebrospinal fluid by isoelectric focusing on thin-layer agarose gels and silver staining.
Isoelectric focusing on thin-layer agarose gels with silver staining was used to detect oligoclonal bands of IgG in unconcentrated cerebrospinal fluid (CSF). Comparison of this procedure with Coomassie blue staining for the detection of oligoclonal bands in CSF yielded concordant results; CSF samples were either positive or negative for oligoclonal bands by both techniques. The silver-staining procedure was capable of detecting oligoclonal bands in CSF samples with IgG levels as low as 2 mg/dL, which was 200 ng/10-microliter sample. False-positive results were not detected in CSF samples with either a high initial IgG level or a low initial IgG level that required concentration before analysis, even by silver staining. This procedure has several advantages over conventional assays: it is fast, needing only five hours; it uses only 10 to 15 microliter of sample; it is easy to perform; and it can be done routinely in the clinical laboratory.
J R
JR
Lane
K J
KJ
Bowles
D E
DE
Normansell
D005782:Gels; D006801:Humans; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid*; D007525:Isoelectric Focusing / Q000379:methods*; D012394:Rosaniline Dyes; D012685:Sepharose; D012834:Silver; D013194:Staining and Labeling
D005782:Gels; D007074:Immunoglobulin G; D012394:Rosaniline Dyes; D012834:Silver; D012685:Sepharose; C004692:coomassie Brilliant Blue