Inflammatory response induced by intrapleural injection of antiserum to IgE in rat. An evaluation of the role of histamine.
Intrapleural injection of antiserum to rat IgE (anti-IgE) into rats resulted in release of histamine from mast cells and rapid effusion of fluid and plasma proteins into the pleural cavity. By 4 hr this was followed by infiltration of neutrophils. These responses were dependent on the amount of anti-IgE injected, and maximal responses were greater than those obtained with compound 48/80. The effusion of fluid and protein, but not the infiltration of cells, was partially suppressed by prior treatment with the H1 histamine receptor antagonist mepyramine (5 mg/kg, s.c.) or the H2 antagonist metiamide (100 mg/kg, s.c.) and was almost totally suppressed (85-88%) when both drugs were administered simultaneously. Neither methysergide (1 and 4 mg/kg, s.c.) nor indomethacin (5 and 10 mg/kg, i.v.) had an effect on the responses to anti-IgE. Although it seemed likely that histamine was a primary mediator of increased vascular permeability, the intrapleural injection of histamine agonists or histamine in large amounts (50 micrograms) provoked a much less intense response than did anti-IgE. The effects of injected histamine may not, therefore, mimic those induced by histamine released from mast cells in situ. The intrapleural injection of histamine releasers such as anti-IgE may serve as a useful model to test the therapeutic efficacy of antihistamine drugs. The present results also confirm previous reports that localized neutrophil infiltration occurs after mast cell degranulation.
Lo
T N
TN
WoldeMussie
E
E
Wilson
N
N
Wu
D
D
Beaven
M A
MA
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D006632:Histamine / Q000502:physiology*; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D006636:Histamine Release; D007106:Immune Sera / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology*; D007249:Inflammation / Q000209:etiology* / Q000473:pathology; D008297:Male; D009504:Neutrophils / Q000473:pathology; D010994:Pleura; D051381:Rats; D011919:Rats, Inbred Strains
D000893:Anti-Inflammatory Agents; D006634:Histamine H1 Antagonists; D007106:Immune Sera; D007073:Immunoglobulin E; D006632:Histamine