Electrophysiological actions of luteinizing hormone-releasing hormone: intracellular studies in the rat hippocampal slice preparation.
The electrophysiological effects of luteinizing hormone-releasing hormone (LHRH) on CA1 pyramidal cells were investigated utilizing intracellular recordings from the in vitro rat hippocampal slice preparation. Bath application of LHRH (10(-7) - 10(-12) M) resulted in several changes in the electrophysiological properties of CA1 neurons. LHRH induced a long-lasting depolarization associated with increased input resistance, a decrease in the afterhyperpolarization (AHP) following a train of action potentials, and a reduction in accommodation of repetitive cell discharge. These effects were blocked by the synthetic LHRH antagonist [Ac-delta-Pro1,pCl-D-Phe2,D-Trp3,6]-LHRH. These findings provide electrophysiological evidence for the role of LHRH as a neurotransmitter/neuromodulator in the hippocampus.
Wong
M
M
Department of Physiology, University of Texas Southwestern Medical Center, Dallas 75235.
Eaton
M J
MJ
Moss
R L
RL
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D051381:Rats; D011919:Rats, Inbred Strains
D007987:Gonadotropin-Releasing Hormone