Growth of Bacillus megaterium in phosphate-limited medium.
Batch cultures of Bacillus megaterium grown in phosphate-limited media were compared with control cultures grown in phosphate-sufficient media. The effects of phosphate limitation on growth were determined by viable cells counts. Intracellular levels of protein, RNA, poly-3-hydroxybutyrate, carbohydrate and oxygen uptake were significantly affected by phosphate limitation. Electron micrographs of sectioned cells revealed differences in the structure; in particular the thick, rigid cell wall was absent from cells grown in phosphate-limited media, and such cells were larger, pleomorphic, and after 2 d were insensitive to lysozyme.
T
T
de la Rubia
J
J
Gonzalez-Lopez
F
F
Ballesteros
A
A
Ramos-Cormenzana
D001410:Bacillus megaterium / Q000032:analysis / Q000254:growth & development* / Q000502:physiology / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000032:analysis; D002473:Cell Wall / Q000648:ultrastructure; D003470:Culture Media; D008854:Microscopy, Electron; D009113:Muramidase / Q000494:pharmacology; D010101:Oxygen Consumption; D010710:Phosphates / Q000378:metabolism / Q000494:pharmacology*; D012329:RNA, Bacterial / Q000032:analysis; D013171:Spores, Bacterial / Q000502:physiology; D013997:Time Factors
D001426:Bacterial Proteins; D003470:Culture Media; D010710:Phosphates; D012329:RNA, Bacterial; D009113:Muramidase