Simultaneous release of several tachykinins and calcitonin gene-related peptide from rat spinal cord slices.
Superfusion of slices of the dorsal half of rat spinal cord in vitro with 10 microM capsaicin or 60 mM potassium lead to the simultaneous release of substance P (SP)-, neurokinin A (NKA)- and calcitonin gene-related peptide (CGRP)-like immunoreactivities (LI). The ratio between capsaicin-stimulated and basal release was higher for CGRP-LI than for SP-LI, indicating that relatively more CGRP is released from sensory nerves, whereas SP is not only released from afferent neurons. High-performance liquid chromatography of NKA-LI revealed several immunoreactive components. One major peak had the retention time of synthetic NKA. A second peak eluted close to the position of synthetic eledoisin. In conclusion, capsaicin releases several bioactive peptides from sensory neurons which may mediate the acute algetic effect of chemical irritants.
Saria
A
A
Gamse
R
R
Petermann
J
J
Fischer
J A
JA
Theodorsson-Norheim
E
E
Lundberg
J M
JM
D000818:Animals; D015740:Calcitonin Gene-Related Peptide; D002211:Capsaicin / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D015288:Neurokinin A; D011188:Potassium / Q000494:pharmacology; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000378:metabolism*; D013373:Substance P / Q000378:metabolism*
D009419:Nerve Tissue Proteins; D013373:Substance P; D015288:Neurokinin A; D015740:Calcitonin Gene-Related Peptide; D011188:Potassium; D002211:Capsaicin