Regulation of gap junctional conductance.
Gap junctional conductance is regulated by the number of channels between coupled cells (the balance between formation and loss of these channels) and by the fraction of these channels that are open (gating mechanisms). A variety of treatments are known to affect junction formation. Adenosine 3',5'-cyclic monophosphate (cAMP) is involved in some cases, and protein synthesis may be required but precursor molecules can also exist. Junction removal occurs both by dispersion of particles and by internalization of junctional membrane. Factors promoting removal are not well understood. A variety of gating mechanisms exist. Coupling may be controlled by changes in conductance of nonjunctional membranes. Several kinds of voltage dependence of junctional conductance are known, but rat ventricular junctions at least are electrically linear. Cytoplasmic acidification decreases conductance of most gap junctions. Sensitivity in rat ventricular myocytes allows modulation of coupling by moderate changes near normal internal pH. Increasing intracellular Ca also decreases junctional conductance, but in the better studied cases sensitivity is much lower to Ca than H. A few data support low sensitivity to Ca in cardiac cells, but quantitative studies are lacking. Higher alcohols such as octanol block junctional conductance in a wide range of tissues including rat ventricular myocytes. An antibody to liver gap junctions blocks junctions between rat ventricular myocytes. Cross reactivity indicates at least partial homology between many gap junctions. Although differences among gap junctions are known, a general physiology is being developed, which may have considerable relevance to normal cardiac function and also to conduction disorders of that tissue.
D C
DC
Spray
R L
RL
White
F
F
Mazet
M V
MV
Bennett
D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology; D004553:Electric Conductivity; D005614:Freeze Fracturing; D005976:Glutaral / Q000494:pharmacology; D006329:Heart Conduction System / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000494:pharmacology; D007473:Ion Channels / Q000502:physiology; D008433:Mathematics; D008854:Microscopy, Electron; D009206:Myocardium / Q000648:ultrastructure; D009469:Neuromuscular Junction / Q000502:physiology*; D014212:Tretinoin / Q000494:pharmacology
D007328:Insulin; D007473:Ion Channels; D002110:Caffeine; D014212:Tretinoin; D000242:Cyclic AMP; D002118:Calcium; D005976:Glutaral