Stimulation of mitochondrial pyruvate transport in rat renal cortex by phenylephrine.
Phenylephrine effect on liver and kidney cortex mitochondrial pyruvate concentration was investigated. While in liver the alpha 1-adrenergic agent produced a decrease in pyruvate content, a significant increase was observed in kidney, even in the presence of 0.5 mM alpha-cyano-4-hydroxy-cinnamate. These changes were not observed when pyruvate was formed by intramitochondrial transamination of alanine, suggesting a role for the pyruvate transport across mitochondrial membranes in the regulation of mitochondrial pyruvate metabolism in kidney cortex. This was corroborated measuring the phenylephrine effect on pyruvate carboxylation.
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Granada, Spain.
J
J
Oliver
M M
MM
Sola
R
R
Salto
A M
AM
Vargas
D000409:Alanine / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D003373:Coumaric Acids / Q000494:pharmacology; D005943:Gluconeogenesis / Q000187:drug effects; D007656:Ketoglutaric Acids / Q000494:pharmacology; D007672:Kidney Cortex / Q000187:drug effects* / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D010656:Phenylephrine / Q000494:pharmacology*; D011773:Pyruvates / Q000493:pharmacokinetics*; D019289:Pyruvic Acid; D051381:Rats; D011919:Rats, Inbred Strains
D003373:Coumaric Acids; D007656:Ketoglutaric Acids; D011773:Pyruvates; D010656:Phenylephrine; C018254:alpha-cyano-3-hydroxycinnamate; D019289:Pyruvic Acid; D000409:Alanine