Chemical and immunochemical characterisation of substance P-like immunoreactivity and physalaemin-like immunoreactivity in a carcinoid tumour.
Extracts of a carcinoid tumour, resected from the mid-portion of the ileum of a patient with no symptoms of endocrine disorder, were associated with a high concentration of substance P-like immunoreactivity. Using reverse-phase high performance liquid chromatography and antisera specifically directed against the C-terminal and N-terminal regions of substance P and against the N-terminal region of physalaemin, the following components were isolated and identified: substance P and its oxidised form, [pGlu5]substance P-(5-11) peptide and its oxidised form, and the oxidised form of physalaemin. The identity of tumour substance P with the undecapeptide was confirmed by amino acid analysis and high performance ion-exchange chromatography. In vitro incubation of tumour tissue from a lymph node metastasis from the same patient with [3H]leucine resulted in incorporation of radioactivity into newly synthesised substance P.
J M
JM
Conlon
G
G
Schäfer
W E
WE
Schmidt
L H
LH
Lazarus
H D
HD
Becker
W
W
Creutzfeldt
D002276:Carcinoid Tumor / Q000032:analysis / Q000097:blood / Q000473:pathology*; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D006801:Humans; D007078:Ileal Neoplasms / Q000032:analysis / Q000097:blood / Q000473:pathology*; D007705:Kinins / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D010803:Physalaemin / Q000032:analysis*; D013373:Substance P / Q000032:analysis* / Q000096:biosynthesis / Q000097:blood
D007705:Kinins; D010803:Physalaemin; D013373:Substance P