RNAs containing B2 repeated sequences are transcribed in the early stages of mouse embryogenesis.
An in situ hybridization technique was used to detect RNAs containing B2 sequences in the early mouse embryo. Accumulation of B2 sequences occurs early from the one cell stage. The level of B2 RNA decreases in the late two cell embryo, and then increases at the moment of second cleavage. In the blastocyst, inner cell mass cells contain more B2 transcripts than trophectoderm cells. In 7.5-day embryos the expression of B2 sequences is restricted to ectoderm and mesoderm. At all stages, transcription of the B2+ strand is greater than B2- strand. We detected B2+ RNAs in the nucleus and cytoplasm, whereas B2- RNAs were present only in the nucleus.
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M
Vasseur
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H
Condamine
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P
Duprey
D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D003433:Crosses, Genetic; D004247:DNA / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D005260:Female; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009693:Nucleic Acid Hybridization; D009865:Oocytes / Q000502:physiology*; D011247:Pregnancy; D012313:RNA / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic
D012313:RNA; D004247:DNA; D004262:DNA Restriction Enzymes