Changes of chromatin conformation around mouse interferon-beta gene associated with induction of interferon synthesis.
Changes of chromatin structure around the mouse interferon-beta gene associated with induction were examined by the use of sensitivity to digestion with DNaseI. Upon induction, the DNaseI-sensitivity of mouse interferon-beta gene was increased and seven DNaseI-hypersensitive sites were mapped. One of them, which was located at approximately 100bp upstream and was insensitive in the uninduced state, showed a marked sensitivity upon induction. Four others, located at 580bp and 430bp upstream, and 1.9kb and 2.2kb downstream, whose levels of DNaseI-sensitivity were very low in the uninduced state, showed significant sensitivity in the induced state. Two remaining sites, located at 7.5kb upstream and 7.6kb downstream, had relatively high DNaseI-sensitivities even in the uninduced state and slightly increased their sensitivities upon induction.
Higashi
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D000818:Animals; D001426:Bacterial Proteins; D002460:Cell Line; D002843:Chromatin / Q000032:analysis*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015245:Deoxyribonuclease BamHI; D003850:Deoxyribonuclease I / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific; D007370:Interferon Type I / Q000235:genetics*; D007372:Interferons / Q000096:biosynthesis*; D007700:Kinetics; D051379:Mice; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000096:biosynthesis; D013329:Structure-Activity Relationship
D001426:Bacterial Proteins; D002843:Chromatin; D007370:Interferon Type I; D012333:RNA, Messenger; D007372:Interferons; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D003850:Deoxyribonuclease I; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific