Measurement of the metabolic interconversion of deuterium-labeled fatty acids by gas chromatography/mass spectrometry.
An analytical method that was developed to analyze deuterium-labeled fatty acids in human blood has been extended to identify labeled fatty acids from C14 to C24 chain length which are formed by metabolic processes such as desaturation, elongation, or shortening of the labeled fatty acids fed. A new computer and a hardware adder have been utilized to assure reliable data acquisition. Relative standard deviations for the analysis of labeled fatty acids were measured at 0.02, 0.03, and 0.04 at the 5%, 1%, and 0.2% levels of the labeled fatty acid methyl esters, respectively. The method makes extensive use of standards and computer processing for accuracy and high productivity. Data from a chylomicron triacylglycerol fraction are included to demonstrate the sensitivity of detection of metabolites formed by desaturation and elongation.
Vegetable Oil Research, U.S. Department of Agriculture, Peoria, Illinois 61604.
W K
WK
Rohwedder
S M
SM
Duval
D J
DJ
Wolf
E A
EA
Emken
D003903:Deuterium; D001330:Electronic Data Processing; D005227:Fatty Acids / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans
D005227:Fatty Acids; D003903:Deuterium