Cloning and sequencing of a human pancreatic tumor mucin cDNA.
A monospecific polyclonal antiserum against deglycosylated human pancreatic tumor mucin was used to select human pancreatic mucin cDNA clones from a lambda gt11 cDNA expression library developed from a human pancreatic tumor cell line. The full-length 4.4-kilobase mucin cDNA sequence included a 72-base pair 5'-untranslated region and a 307-base pair 3'-untranslated region. The predicted amino acid sequence for this cDNA revealed a protein of 122,071 daltons containing 1,255 amino acid residues of which greater than 60% were serine, threonine, proline, alanine, and glycine. Approximately two-thirds of the protein sequence consisted of identical 20-amino acid tandem repeats which were flanked by degenerate tandem repeats and nontandem repeat sequences on both the amino-terminal and carboxyl-terminal ends. The amino acid sequence also contained five putative N-linked glycosylation sites, a putative signal sequence and transmembrane domain, and numerous serine and threonine residues (potential O-linked glycosylation sites) outside and within the tandem repeat position. The cDNA and deduced amino acid sequence of the pancreatic mucin sequence was over 99% homologous with a mucin cDNA sequence derived from breast tumor mucin, even though the native forms of these molecules are quite distinct in size and degree of glycosylation.
Lan
M S
MS
Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina 27710.
Batra
S K
SK
Qi
W N
WN
Metzgar
R S
RS
Hollingsworth
M A
MA
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000235:genetics* / Q000302:isolation & purification; D015723:Gene Library; D006801:Humans; D007106:Immune Sera; D008969:Molecular Sequence Data; D009077:Mucins / Q000235:genetics*; D010190:Pancreatic Neoplasms / Q000235:genetics*; D014176:Protein Biosynthesis; D012334:RNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D012091:Repetitive Sequences, Nucleic Acid
D004273:DNA, Neoplasm; D007106:Immune Sera; D009077:Mucins; D012334:RNA, Neoplasm