Nuclear patterns of cyclin (PCNA) antigen distribution subdivide S-phase in cultured cells--some applications of PCNA antibodies.
Immunofluorescence studies using PCNA autoantibodies specific for the proliferation-sensitive protein cyclin have revealed dramatic changes in the nuclear distribution of this protein during the S-phase of normal and transformed cells. Patterns of cyclin antigen distribution subdivide S-phase and have provided new cell cycle landmarks. Some of these (nucleolar exclusion or staining), mimic topographical patterns of DNA synthesis thus arguing for a role of this protein in some specific aspect of DNA replication. Cells outside S-phase (G0 included) stain only weakly with PCNA antibodies, stressing the usefulness of this reagent for identifying proliferating cells (S-phase cells) of both normal and malignant origins.
J E
JE
Celis
P
P
Madsen
S
S
Nielsen
A
A
Celis
D000818:Animals; D001323:Autoantibodies / Q000276:immunology*; D002453:Cell Cycle; D002455:Cell Division; D002467:Cell Nucleus / Q000276:immunology*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication; D006801:Humans; D007399:Interphase; D007951:Leukemia, Myeloid / Q000473:pathology; D009698:Nucleoproteins / Q000032:analysis* / Q000276:immunology; D018809:Proliferating Cell Nuclear Antigen; D013194:Staining and Labeling
D001323:Autoantibodies; D009698:Nucleoproteins; D018809:Proliferating Cell Nuclear Antigen; D004247:DNA