The valency state of absorbed iron appearing in the portal blood and ceruloplasmin substitution.
(1) Attempts to determine the redox-state of the absorbed iron, which appeared in the portal blood when the free iron-binding capacity was previously saturated, indicate that about 30-90% of this iron was in the ferrous state. This effect was particularly prominent after luminal administration of ferrous iron, but was also seen when iron was given in the ferric state. (2) Total iron absorption is significantly higher in ceruloplasmin-substituted copper-deficient animals as compared to copper-deficient controls. (3) The appearance rate of absorbed iron in the portal blood of copper-deficient animals increased several times immediately after the intravenous infusion of ceruloplasmin. (5) The distribution of absorbed iron was changed due to the ceruloplasmin substitution: it was increased in the reticulocytes (+66%), plasma (+400%) and the body (+112%), whereas in the liver it was decreased by about 78%. (5) In iron-deficient rats intravenously injected ceruloplasmin did not increase iron absorption. (6) The conclusion was drawn that, as for the entrance into the mucosa from the luminal side, also for the release at the contraluminal side into the portal blood, the ferrous state of iron is favoured and that ceruloplasmin accelerates the release into the portal blood by catalyzing the oxidation of ferrous iron due to its high Fe(II): oxygen oxidoreductase (EC 1.16.3.1) activity.
Institut für Pharmakologie und Toxikologie, Universität des Saarlandes, Homburg/Saar, Federal Republic of Germany.
P
P
Wollenberg
R
R
Mahlberg
W
W
Rummel
D000818:Animals; D002570:Ceruloplasmin / Q000502:physiology*; D003300:Copper / Q000172:deficiency; D005260:Female; D005290:Ferric Compounds / Q000097:blood*; D005296:Ferrous Compounds / Q000097:blood*; D007408:Intestinal Absorption; D007421:Intestine, Small / Q000098:blood supply; D000090463:Iron Deficiencies; D007504:Iron Radioisotopes; D007700:Kinetics; D008643:Mesentery / Q000098:blood supply; D011169:Portal Vein / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains
D005290:Ferric Compounds; D005296:Ferrous Compounds; D007504:Iron Radioisotopes; D003300:Copper; D002570:Ceruloplasmin