Visual tests for urinary amylase investigated in the routine laboratory.
Ninety-seven fresh urine specimens were tested in the routine laboratory with the Rapignost Amylase test strip, and the results were compared to those obtained using Phadebas Amylase test tablets to investigate the transferability of the results obtained by the Rapignost method to those of the Phadebas method under routine conditions. The fraction of conflicting negative results of Phadebas-positive specimens was 9% and the corresponding fraction of conflicting positive results was 13%. An attempt to improve the transferability by changing the comparison scale slightly did not succeed. However, a visual binary test based on 180 s incubation at 37 degrees C of 400 microliters urine in a suspension of a Phadebas Amylase test tablet seemed more suitable in selection of specimens with Phadebas amylase activity less than 2000 U/l (upper limit of the reference interval). These specimens amounted to approximately 75% of all amylase specimens in our laboratory.
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Uldall
D000681:Amylases / Q000652:urine*; D006801:Humans; D011933:Reagent Kits, Diagnostic; D011934:Reagent Strips
D011933:Reagent Kits, Diagnostic; D011934:Reagent Strips; D000681:Amylases