Characterization of a monoclonal antibody that recognizes a determinant unique to human haemoglobin beta-chain.
Methodologies are described for the production and characterization of monoclonal antibodies to human haemoglobin. Three monoclonal antibodies are described, two of which recognize distinct determinants on the alpha-chain subunit. A third monoclonal antibody, Hb-2d, recognizes a determinant expressed on human beta-chain. The Hb-2d determinant is shared by human and baboon haemoglobins, but is not expressed by haemoglobins from beef, goose, pig, rabbit, sheep, dog, rat or mouse. Monoclonal antibody Hb-2d will bind to haemoglobin A2 but not to foetal haemoglobin suggesting that delta-but not gamma-chain also expresses the Hb-2d determinant. The results of testing a limited panel of human haemoglobin variants is presented.
Krco
C J
CJ
Gorzynski
T J
TJ
Beito
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D000918:Antibody Specificity; D002417:Cattle; D002645:Chickens / Q000097:blood; D010856:Columbidae / Q000097:blood; D003429:Cross Reactions; D004285:Dogs; D000939:Epitopes / Q000032:analysis*; D005777:Geese / Q000097:blood; D006041:Goats / Q000097:blood; D006454:Hemoglobins / Q000032:analysis* / Q000276:immunology; D006455:Hemoglobins, Abnormal / Q000032:analysis; D006801:Humans; D051379:Mice; D010215:Papio / Q000097:blood; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats; D013552:Swine / Q000097:blood
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006454:Hemoglobins; D006455:Hemoglobins, Abnormal