Monoclonal antibodies to Nocardia asteroides and Nocardia brasiliensis antigens.
Nocardia asteroides and Nocardia brasiliensis whole-cell extracts were used as antigens to generate monoclonal antibodies (MAbs). Six stable hybrid cell lines secreting anti-Nocardia spp. MAbs were obtained. These were characterized by enzyme-linked immunosorbent assay, Western blot (immunoblot), and immunofluorescence assay. Although all the MAbs exhibited different degrees of cross-reactivity with N. asteroides and N. brasiliensis antigens as well as with culture-filtrate antigens from Mycobacteria spp., they have the potential for use as reagents in the purification of Nocardia antigens.
Jiménez
T
T
Department of Microbiology and Medical Zoology, School of Medicine, University of Puerto Rico, San Juan 00936.
Díaz
A M
AM
Zlotnik
H
H
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D015153:Blotting, Western; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D009161:Mycobacterium / Q000276:immunology; D009615:Nocardia / Q000276:immunology*; D009617:Nocardia Infections / Q000175:diagnosis; D009616:Nocardia asteroides / Q000276:immunology
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial