The preparation and use of a carrier-bound acceptor for the determination of sialyl transferase activity in serum.
Agarose-bound asialofetuin functions as an insoluble sialyl group acceptor in a simplified assay for sialyl transferase (CMP-neuraminate: D-galactosyl-glycoprotein N-acetylneuraminyl transferase; EC 2.4.99.1) in serum. Since sialyl transferase levels in serum are elevated in a large number of malignant conditions, the simplified assay is of use for clinical monitoring in tumour therapeutic programmes.
Ganzinger
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Unger
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D001212:Asialoglycoproteins; D004796:Clinical Enzyme Tests; D060748:Fetuins; D006801:Humans; D007700:Kinetics; D009369:Neoplasms / Q000175:diagnosis* / Q000201:enzymology; D012016:Reference Values; D012685:Sepharose; D012799:Sialyltransferases / Q000097:blood*; D014166:Transferases / Q000097:blood*; D000509:alpha-Fetoproteins
D001212:Asialoglycoproteins; D060748:Fetuins; D000509:alpha-Fetoproteins; C017029:asialofetuin; D012685:Sepharose; D014166:Transferases; D012799:Sialyltransferases