A 90,000-dalton binding protein common to both steroid receptors and the Rous sarcoma virus transforming protein, pp60v-src.
Previous studies have shown that the avian progesterone receptor, when in the nontransformed 8 S state, is complexed to another cellular protein having a molecular weight of 90,000. In this report, we show that this receptor-binding protein is indistinguishable from the 90,000-dalton protein which associates in a complex with the Rous sarcoma virus transforming protein, pp60v-src. This identity was established by the following criteria. 1) Monoclonal antibodies directed against the pp60v-src-associated 90-kDa protein recognized the 90-kDa progesterone receptor binding protein in an immunoblot assay. Conversely, monoclonal antibodies that recognize the progesterone receptor binding protein bind to the 90-kDa protein which complexes with pp60v-src. 2) Peptide maps prepared from the 90-kDa proteins immunoprecipitated from chicken cells with monoclonal antibodies directed against either the 90-kDa receptor binding protein or the 90-kDa pp60v-src-associated protein were indistinguishable. 3) Preincubation of the progesterone receptor complex with monoclonal antibodies prepared against the pp60v-src-associated protein caused a shift in the sedimentation of the progesterone receptor. Previous studies have established that the pp60v-src-associated protein is indistinguishable from one of the major heat shock proteins which are induced under a variety of stress conditions in eukaryotic cells. These present studies implicate a new role for this 90-kDa protein in the action of steroid hormones.
S
S
Schuh
W
W
Yonemoto
J
J
Brugge
V J
VJ
Bauer
R M
RM
Riehl
W P
WP
Sullivan
D O
DO
Toft
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism*; D002642:Chick Embryo; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D005260:Female; D006360:Heat-Shock Proteins / Q000378:metabolism; D007158:Immunologic Techniques; D007163:Immunosorbent Techniques; D008970:Molecular Weight; D015688:Oncogene Protein pp60(v-src); D010057:Oviducts / Q000032:analysis; D011980:Receptors, Progesterone / Q000378:metabolism*; D012191:Retroviridae Proteins / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D000939:Epitopes; D006360:Heat-Shock Proteins; D011980:Receptors, Progesterone; D012191:Retroviridae Proteins; D015688:Oncogene Protein pp60(v-src)