In vivo covalent binding of trans-4-hydroxy-2-hexenal to rat liver macromolecules.
Trans-4-hydroxy-2-hexenal (t-4HH), a metabolite isolated from the macrocyclic pyrrolizidine alkaloid senecionine, was tritiated to perform in vivo covalent binding studies. The in vivo binding of [3H]t-4HH to rat liver DNA, RNA and protein was investigated. No significant binding to hepatic DNA or RNA could be detected, but levels of 429 +/- 139 pmol/mg were found covalently bound to hepatic proteins 16 h after exposure. Levels as high as 916 pmol/mg protein were detected 2 h after administration of [3H]t-4HH via the hepatic portal.
Grasse
L D
LD
Lamé
M W
MW
Segall
H J
HJ
D000447:Aldehydes / Q000138:chemical synthesis / Q000378:metabolism*; D000818:Animals; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D011485:Protein Binding; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D012313:RNA / Q000302:isolation & purification / Q000378:metabolism*; D011865:Radioisotope Dilution Technique; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium
D000447:Aldehydes; D011506:Proteins; D014316:Tritium; C045934:4-hydroxy-2-hexenal; D012313:RNA; D004247:DNA