In vitro and in vivo studies of heparinized-collageno-elastic tubes.
Heparin was covalently coupled to collageno-elastic grafts (CET) derived from lamb carotid arteries, by using the crosslinking agent 1-ethyl-3 (3-dimethyl-aminopropyl) carbodiimide (EDC). The collagenous grafts were pretreated with various aminating agents in order to enhance the number of available binding sites on the collagen surface. By varying the EDC/heparin weight ratio, the pH of the immobilization media, and the pretreatment agent, a global search pattern maximized heparin loading at 3.90 +/- 0.36 USP heparin/cm2 collagenous graft surface when the EDC/heparin ratio was 2:1 at a pH of 1.5 with 1 M hydroxylamine sulfate as the pretreatment agent. Heparinized CETs were superior to nonheparinized CETs by exhibiting both enhanced antiplatelet activity in using an in vitro differential recirculation reactor with chromium-51 tagged platelets and enhanced patency when interposed in canine carotid arteries. Both antiplatelet activity and patency duration for heparinized CETs were independent of heparin loading.
Senatore
F
F
Department of Chemical Engineering, Texas Tech University, Lubbock 79409.
Shankar
H
H
Chen
J H
JH
Avantsa
S
S
Feola
M
M
Posteraro
R
R
Blackwell
E
E
D000818:Animals; D001705:Bioprosthesis; D001807:Blood Vessel Prosthesis; D002339:Carotid Arteries; D055598:Chemical Phenomena; D002621:Chemistry; D003094:Collagen / Q000378:metabolism*; D004285:Dogs; D005260:Female; D006493:Heparin / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D010975:Platelet Aggregation Inhibitors; D012756:Sheep
D010975:Platelet Aggregation Inhibitors; D006493:Heparin; D003094:Collagen