Glycoprotein glycans that inhibit adhesion of Escherichia coli mediated by K99 fimbriae: treatment of experimental colibacillosis.
Calf diarrhea due to infection by enterotoxigenic Escherichia coli was treated by administration of glycoprotein glycans derived from bovine plasma. The glycan moieties of the nonimmunoglobulin fraction of plasma mimicked the oligosaccharide moiety of intestinal receptors recognized by K99 pili. These glycoprotein glycans inhibited adhesion of E. coli K99+ ST+ to erythrocyte glycoconjugates in vitro, and they protected colostrum-deprived newborn calves against lethal doses of enterotoxigenic E. coli (10(10) bacteria). Adhesion of bacteria to the intestines (duodenum, jejunum, and ileum) was significantly reduced (by 2 orders of magnitude) in treated calves.
Faculté des Sciences, Université de Limoges, France.
M
M
Mouricout
J M
JM
Petit
J R
JR
Carias
R
R
Julien
D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects; D002240:Carbohydrate Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000628:therapy; D003967:Diarrhea / Q000628:therapy / Q000662:veterinary; D004927:Escherichia coli Infections / Q000628:therapy*; D005243:Feces / Q000382:microbiology; D006020:Glycopeptides / Q000008:administration & dosage*; D006384:Hemagglutination / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007413:Intestinal Mucosa / Q000382:microbiology; D008969:Molecular Sequence Data; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D013329:Structure-Activity Relationship
D006020:Glycopeptides; D012964:Sodium; D011188:Potassium