[Effect of treatment with clindamycin, erythromycin, rifamycin or gentamicin on the ingestion capacity of peritoneal macrophages in mice].
The effect of treatment with clindamycin, erythromycin, rifamycin and gentamicin on the ingestion capacity of the mouse peritoneal macrophage was studied. Female six-eight week old OF1 mice were treated with minimum and maximum doses clinically used for the different antibiotics (15 and 40 mg/kg/day of clindamycin, 15 and 57.5 mg/kg/day of erythromycin, 10 and 30 mg/kg/day of rifamycin, 3 and 6 mg/kg/day of gentamicin). Two treatment periods of 72 hours and one week were assayed for each antibiotic-dose combination. Antibiotic was administered twice daily, every twelve hours. Twelve hours after the last dose was given, macrophages were obtained through peritoneal lavage and a kinetic study of Candida albicans blastospore ingestion was made. One week treatment with 15 mg/kg/day and 72 hours treatment with 57.5 mg/kg/day of erythromycin produced a significant ingestion enhancement whereas one week treatment with 57.5 mg/kg/day of erythromycin and all treatments with gentamicin gave rise to an ingestion depression. 72 hours treatment with 15 and 40 mg/kg/day and one week treatment with 15 mg/kg/day of clindamycin, 15 mg/kg/day of erythromycin and 10 and 30 mg/kg/day of rifamycin did not modify macrophage ingestion capacity. One week treatment with 40 mg/kg/day of clindamycin and 10 and 30 mg/kg/day of rifamycin gave rise to an acceleration of the ingestion process.
Amurrio
C
C
Département de Microbiologie et Immunologie, Faculté de Médecine, Université du Pays Basque, Lejona, Espagne.
Lewden
S
S
Nicolas
R
R
Gonzalez
P
P
Cañavate
M L
ML
Cisterna
R
R
D000818:Animals; D002176:Candida albicans; D002981:Clindamycin / Q000008:administration & dosage / Q000494:pharmacology*; D004334:Drug Administration Schedule; D004917:Erythromycin / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D005839:Gentamicins / Q000008:administration & dosage / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects*; D051379:Mice; D008815:Mice, Inbred Strains; D010529:Peritoneal Cavity / Q000166:cytology / Q000382:microbiology; D010587:Phagocytosis / Q000187:drug effects; D012294:Rifamycins / Q000008:administration & dosage / Q000494:pharmacology*
D005839:Gentamicins; D012294:Rifamycins; D002981:Clindamycin; D004917:Erythromycin
Effet du traitement par la clindamycine, l'érythromycine, la rifamycine ou la gentamicine sur la capacité d'ingestion du macrophage péritonéal de souris.