Neurohypophyseal hormone regulation of endothelin secretion from rabbit endometrial cells in primary culture.
Immunoreactive endothelin-1 (IR-ET-1) was detected in the cultured medium from endometrial but not myometrial cells of rabbits in primary culture using a specific radioimmuno assay (RIA). Similar results were obtained with a radioreceptor assay using myometrial membranes. In a reverse-phase HPLC synthetic ET-1 and IR-ET-1 of the extract medium from endometrial cells revealed essentially the same elution profiles, as determined by RIA. Two selective agonists of oxytocin (OT) or V1 vasopressin (VP) receptors produced, respectively, a 6- and 2-fold increase of IR-ET-1 release from endometrial cells. These effects were completely reversed by the addition of two specific antagonists of OT and V1 VP receptors. Our results indicate that ET-1 is produced and released in the culture medium of rabbit endometrial cells in primary culture. The release of ET-1 is under receptor-specific control by neurohypophyseal hormones.
Department of Clinical Physiopathology, University of Florence, Italy.
C
C
Orlando
M L
ML
Brandi
A
A
Peri
S
S
Giannini
G
G
Fantoni
E
E
Calabresi
M
M
Serio
M
M
Maggi
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D004717:Endometrium / Q000166:cytology / Q000378:metabolism*; D016232:Endothelins; D004730:Endothelium, Vascular; D005260:Female; D010455:Peptides / Q000378:metabolism*; D010908:Pituitary Hormones, Anterior / Q000502:physiology*; D011817:Rabbits; D011863:Radioimmunoassay; D013997:Time Factors
D003470:Culture Media; D016232:Endothelins; D010455:Peptides; D010908:Pituitary Hormones, Anterior