Mechanism of RNA redistribution of the 17,000g postmitochondrial supernatant after incubation at 37 degrees C and its impact on other related biochemical investigations.
Evidence for stripping of ribosomes from RER in the 17,000g PMS of the liver of the cancer patient was obtained in the 1.35 M region which is the region between the SER and RER. RNA/protein ratios for the SER, 1.35 M region, and RER of 0.001, 0.083, and 0.235, respectively, for this liver are consistent with the degranulation of RER compared with RNA/protein ratios for SER and RER from normal livers of 0.025 +/- 0.003, and 0.35 + 0.030, respectively. A RNA/protein ratio of 0.235 was obtained for the RER region of the cancer patient. The EM revealed that the region of the RER in the cancer liver was ribosomal and not at all RER. This ribosomal material is reminiscent of the ribosomes banding in the 1.35- to 2 M domain of the RER isolated from the reconstitution experiments (SER + polyribosomes + supernatant). It was suggested that the ribosomal material isolated from the cancer liver could therefore be indicative of polyribosomal lesion or degradation. The 0.25-1.35 M interface (SER), 1.35 M region, and 1.35-2 M interface (RER) characteristics are therefore exploitable for diagnostic potential and further understanding of the molecular basis of cancer.
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D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D002458:Cell Fractionation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000379:methods; D004721:Endoplasmic Reticulum / Q000032:analysis / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D006801:Humans; D007668:Kidney / Q000032:analysis / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D008099:Liver / Q000032:analysis / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D008113:Liver Neoplasms / Q000032:analysis* / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D009152:Mutagenicity Tests; D011132:Polyribosomes / Q000378:metabolism; D012313:RNA / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000032:analysis / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013223:Statistics as Topic; D013696:Temperature; D013997:Time Factors
D012334:RNA, Neoplasm; D012335:RNA, Ribosomal; D012313:RNA