High affinity receptors for bombesin/GRP-like peptides on human small cell lung cancer.
The binding of a radiolabeled bombesin analogue to human small cell lung cancer (SCLC) cell lines was investigated. (125I-Tyr4)bombesin bound with high affinity (Kd = 0.5 nM) to a single class of sites (2,000/cell) using SCLC line NCI-H446. Binding was reversible, saturable and specific. The pharmacology of binding was investigated using NCI-H466 and SCLC line NCI-H345. Bombesin and structurally related peptides, such as gastrin releasing peptide (GRP), but not other peptides, such as substance P or vasopressin, inhibited high affinity (125I-Tyr4)BN binding activity. Finally, the putative receptor, a 78,000 dalton polypeptide, was identified by purifying radiolabeled cell lysates on bombesin or GRP affinity resins and then displaying the bound polypeptides on sodium dodecylsulfate polyacrylamide gels. Because SCLC both produces bombesin/GRP-like peptides and contains high affinity receptors for these peptides, they may function as important autocrine regulatory factors for human SCLC.
T W
TW
Moody
D N
DN
Carney
F
F
Cuttitta
K
K
Quattrocchi
J D
JD
Minna
D001839:Bombesin / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D018288:Carcinoma, Small Cell / Q000378:metabolism*; D002460:Cell Line; D002846:Chromatography, Affinity; D019886:Gastrin-Releasing Peptide; D006801:Humans; D007700:Kinetics; D008175:Lung Neoplasms / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism* / Q000494:pharmacology*; D018004:Receptors, Bombesin; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000378:metabolism*; D013373:Substance P / Q000494:pharmacology; D014667:Vasopressins / Q000494:pharmacology
D010455:Peptides; D018004:Receptors, Bombesin; D011956:Receptors, Cell Surface; D014667:Vasopressins; D013373:Substance P; C035891:bombesin, Tyr(4)-; D019886:Gastrin-Releasing Peptide; D001839:Bombesin