Molecular mapping of idiotopes of anti-arsonate antibodies.
As part of understanding molecular function in structural terms, we have been attempting to map the idiotypic topography of specific anti-arsonate (Ars) antibodies. A panel of anti-Ars hybridomas of which the complete primary sequences are known were used. These molecules show a varied reactivity profile with a panel of monoclonal anti-idiotypic antibodies. By judicious chain recombination experiments and chemical modifications that altered this reactivity profile, we were able to identify particular amino acid residues or discreet regions of anti-Ars antibodies as having crucial roles in the expression of idiotypic determinants. Idiotopes were mapped to the heavy chain second hypervariable region and D segment, and to the light chain first and third hypervariable regions.
Jeske
D
D
Milner
E C
EC
Leo
O
O
Moser
M
M
Marvel
J
J
Urbain
J
J
Capra
J D
JD
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D001391:Azo Compounds / Q000032:analysis*; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000032:analysis; D007130:Immunoglobulin Idiotypes / Q000032:analysis* / Q000276:immunology; D007147:Immunoglobulin Light Chains / Q000032:analysis; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D011193:Potassium Iodide; D013387:Succinic Anhydrides; D010132:p-Azobenzenearsonate / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D001391:Azo Compounds; D000939:Epitopes; D007143:Immunoglobulin Heavy Chains; D007130:Immunoglobulin Idiotypes; D007147:Immunoglobulin Light Chains; D013387:Succinic Anhydrides; D011193:Potassium Iodide; C031801:succinic anhydride; D010132:p-Azobenzenearsonate