Use of a cloned gene involved in candicidin production to discover new polyene producer Streptomyces strains.
A p-aminobenzoic synthase gene (pabS) from Streptomyces griseus IMRU 3570 involved in candicidin production was used as probe to find new aromatic polyene producing Streptomyces strains. The pab gene hybridizes with 6 out of 16 Streptomyces strains, and those strains which hybridize turned out to be polyene producers. Such strains were never before described as polyene producers.
Gil
J A
JA
Area de Microbiologia, Facultad de Biología, Universidad de León, Spain.
Criado
L M
LM
Alegre
T
T
Martín
J F
JF
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D015139:Blotting, Southern; D002174:Candicidin / Q000096:biosynthesis*; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D005798:Genes, Bacterial; D010957:Plasmids; D015183:Restriction Mapping; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism; D000637:Transaminases / Q000235:genetics*; D014169:Transformation, Bacterial
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D002174:Candicidin; C022309:PABA synthase; D000637:Transaminases