Light induced shift and binding of S-antigen in retinal rods.
S-antigen has been quantitated in bovine and rat retina by electroimmunoassay. The molar ratios S-antigen to rhodops in in photoreceptor cells were close to 1:1. Immunofluorescence studies show that light induces a shift of S-antigen towards the rod outer segments where it concentrates. Assays indicate that S-antigen becomes largely water insoluble but detergent soluble under these conditions. On basis of previous ultrastructural and present results, this has been interpreted as a light induced binding of S-antigen to the rod outer segment membranes. The data support evidence from literature that S-antigen interacts with (rhod) ops in and we conclude that S-antigen plays a major role in the phototransduction process.
R M
RM
Broekhuyse
E F
EF
Tolhuizen
A P
AP
Janssen
H J
HJ
Winkens
D000222:Adaptation, Physiological; D000818:Animals; D000941:Antigens / Q000032:analysis / Q000276:immunology*; D019393:Arrestin; D005455:Fluorescent Antibody Technique; D007122:Immunoelectrophoresis; D008027:Light; D010786:Photoreceptor Cells / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling
D000941:Antigens; D019393:Arrestin