Resistance of human follicular oocytes to parthenogenetic activation: DNA distribution and content in oocytes maintained in vitro.
Sixty-nine human oocytes were subjected to various parthenogenetic stimuli but no activation was observed. Analysis of Feulgen-stained DNA (F-DNA) distribution and content showed anomalies of chromosomal material in 36% of oocytes at 24-75 h after retrieval. A significant loss of F-DNA was noticed in apparently normal metaphase II oocytes remaining in culture for 2-3 days.
Royal Women's Hospital, Carlton, Australia.
J W
JW
Abramczuk
A
A
Lopata
D002478:Cells, Cultured; D003080:Cold Temperature; D004247:DNA / Q000032:analysis*; D000431:Ethanol / Q000494:pharmacology; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology; D008297:Male; D008540:Meiosis; D009865:Oocytes / Q000032:analysis / Q000502:physiology*; D010312:Parthenogenesis; D013094:Spermatozoa / Q000502:physiology
D000431:Ethanol; D004247:DNA; D006821:Hyaluronoglucosaminidase