Reduced thrombogenicity of polymers having phospholipid polar groups.
The thrombogenicity of polymers having a phospholipid polar group, poly(2-methacryloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (BMA)), was evaluated by a microsphere-column method with attention to the activation and adhesion of platelets on the polymer surface. When citrated platelet-rich plasma (PRP) contacted with the polymers, a large number of platelets adhered and aggregated on poly(BMA). The number of adherent platelets decreased and deformation and aggregation were suppressed with increasing MPC composition. The same tendency was noted when Ca2(+)-re-added PRP came in contact with the polymers. In the case of poly(MPC-co-BMA) with 0.320 mole fraction of MPC, activation of platelets and formation of fibrin were completely suppressed. Therefore, MPC moieties in the polymer play an important role in the reduction of thrombogenicity of the polymer.
Institute for Medical and Dental Engineering, Tokyo Medical and Dental University, Japan.
K
K
Ishihara
R
R
Aragaki
T
T
Ueda
A
A
Watenabe
N
N
Nakabayashi
D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D066298:In Vitro Techniques; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010743:Phospholipids; D010973:Platelet Adhesiveness; D011092:Polyethylene Glycols; D011108:Polymers / Q000009:adverse effects*; D011109:Polymethacrylic Acids / Q000009:adverse effects; D011817:Rabbits; D013499:Surface Properties; D013927:Thrombosis / Q000209:etiology* / Q000517:prevention & control
D001672:Biocompatible Materials; D010743:Phospholipids; D011108:Polymers; D011109:Polymethacrylic Acids; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols