[Protamine precursors in human spermatozoa].
Basic proteins isolated from human sperm nuclei are highly heterogeneous. Three groups of nuclear basic proteins have been characterized: somatic-type as well as testis-specific histones, protamines and basic proteins with an electrophoretic mobility which is intermediate between that of histones and that of protamines. Human protamines can be separated into 2 protein families with different amino acid composition and amino-acid sequence. Protamines HP1 differ in their degree of phosphorylation. Protamines HP2, 3 and 4 differ by their amino-terminal sequence. Intermediate basic proteins (HPI1, HPI2, HPS1, HPS2) share a common C-terminal sequence of 54 residues identical to the amino-acid sequence of protamine HP3; only their N-terminal regions are different. Taking into account these structural homologies, the intermediate basic protein HPI1 appears as a precursor of protamines HP2 and HP3.
Université Paris-Val de Marn, laboratoire de biologie cellulaire, Créteil, France.
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Arkhis
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Sautière
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011479:Protamines / Q000032:analysis*; D011498:Protein Precursors / Q000032:analysis*; D013094:Spermatozoa / Q000032:analysis*
D011479:Protamines; D011498:Protein Precursors; C046533:protamine P1; C048529:protamine P2; C052196:protamine P3
Les précurseurs des protamines dans le spermatozoïde humain.