Molecular analysis of structural protein genes of the Yamagata-1 strain of defective subacute sclerosing panencephalitis virus. III. Nucleotide sequence of the hemagglutinin gene.
The full-length cDNA corresponding to the mRNA for the hemagglutinin (H) protein of the Yamagata-1 strain of the subacute sclerosing panencephalitis (SSPE) virus was cloned and the nucleotide sequence was determined. The mRNA corresponding to the H protein was composed of 1952 nucleotides and contained a single large open reading frame, which encoded 620 amino acids with a predicted molecular weight of 69,723. This cDNA clone expressed the H protein in Cos 7 cells, and the transfected cells showed hemadsorption. The nucleotide and amino-acid sequence homology with the Edmonston strain of MV were 98.0% and 96.6%, respectively. The deduced amino acid sequence had a single hydrophobic domain near the N-terminus that was long enough to serve as an anchor in the membrane. Five potential glycosylation sites were found on the H protein at identical positions as in the H protein of MV. Cysteine and proline were located at almost identical positions as those of the H protein of MV. In addition, monoclonal antibody study revealed that three epitopes, including the domains that were involved in the biological activities of the H protein of MV, were conserved on the Yamagata-1 strain. These results suggested that the H protein of the Yamagata-1 strain of defective SSPE virus is structurally and functionally similar to that of the Edmonston strain of MV.
Laboratory Animal Research Center, University of Tokyo, Japan.
K
K
Komase
T
T
Haga
Y
Y
Yoshikawa
T A
TA
Sato
K
K
Yamanouchi
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D000940:Antigenic Variation / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000032:analysis; D005814:Genes, Viral; D015698:Genomic Library; D006389:Hemagglutinins, Viral / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008459:Measles virus / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis; D013190:SSPE Virus / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D015678:Viral Structural Proteins / Q000235:genetics* / Q000276:immunology
D000911:Antibodies, Monoclonal; D004279:DNA, Viral; D006389:Hemagglutinins, Viral; D012333:RNA, Messenger; D015678:Viral Structural Proteins