Rho-dependent transcription termination in the tryptophanase operon leader region of Escherichia coli K-12.
Recent studies have suggested that expression of the tryptophanase (tna) operon of Escherichia coli is subject to transcription termination-antitermination control (V. Stewart and C. Yanofsky, J. Bacteriol. 164:731-740, 1985). In vivo studies have indicated that the transcribed leader region, tnaL, contains a site or sites of rho-dependent transcription termination (rho is the polypeptide product of the gene rho). We now report direct in vitro evidence that tnaL contains rho-dependent termination sites. In vivo termination appeared to occur at the rho-dependent termination sites identified in vitro. Transcription pausing analyses correlated sites of pausing in tnaL with sites of rho-dependent termination.
V
V
Stewart
R
R
Landick
C
C
Yanofsky
D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics*; D008190:Lyases / Q000235:genetics*; D009154:Mutation; D009690:Nucleic Acid Conformation; D009876:Operon; D011401:Promoter Regions, Genetic; D012329:RNA, Bacterial / Q000032:analysis; D012234:Rho Factor / Q000502:physiology*; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic; D014368:Tryptophanase / Q000235:genetics*
D012329:RNA, Bacterial; D012234:Rho Factor; D014157:Transcription Factors; D008190:Lyases; D014368:Tryptophanase