Peanut agglutinin. A useful marker for histiocytosis X and interdigitating reticulum cells.
We studied the peanut agglutinin (PNA) staining patterns of histiocytosis X (H-X) (three cases, including one case of Letterer-Siwe disease) and well-characterized cases of malignant histiocytosis (two cases) and monoblastic leukemia (one case) in paraffin-embedded specimens. H-X cells showed staining identical to that of interdigitating reticulum cells, with unique paranuclear and cell surface deposits of reaction products. Thus, H-X cells were easily distinguished from benign macrophage-histiocytes, which show diffuse cytoplasmic staining, and malignant cells of malignant histiocytosis and monoblastic leukemia, which did not stain with PNA, PNA staining proved more useful than Leu-M1 antibody for this purpose, since neither interdigitating reticulum cells in ten reactive lymph nodes nor Langerhans'-type cells in three cases of H-X were stained with Leu-M1 antibody. These observations support a close relationship between H-X cells and interdigitating reticulum cells, and demonstrate the usefulness of PNA for the identification of these cells in paraffin-embedded material.
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HJ
Ree
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Kadin
D000328:Adult; D005260:Female; D006644:Histiocytes / Q000473:pathology; D006646:Histiocytosis, Langerhans-Cell / Q000473:pathology*; D006801:Humans; D007223:Infant; D037102:Lectins; D007938:Leukemia / Q000473:pathology; D008206:Lymphatic Diseases / Q000473:pathology; D008264:Macrophages / Q000473:pathology; D008297:Male; D008875:Middle Aged; D019887:Peanut Agglutinin; D009418:S100 Proteins / Q000032:analysis; D013194:Staining and Labeling
D037102:Lectins; D019887:Peanut Agglutinin; D009418:S100 Proteins