Transmission and scanning electron microscopic study of chlordecone (Kepone) induced changes in the male mouse choroid plexus.
To investigate the effects of the estrogenic insecticide chlordecone on the morphology of mouse choroid plexus, 4 different doses (100.0, 250.0, 500.0 and 1000.0 micrograms) of the chemical were administered intraperitoneally to groups of adult males. Concurrent with the chlordecone treatments, another group of males received 10.0 micrograms estradiol-17 beta; the control group was treated with sesame oil vehicle. After 15 days of daily chemical injections, the mice were terminated and the choroid plexus from fourth ventricle examined morphologically. Scanning (SEM) and transmission (TEM) electron microscopic examination of control choroid plexus showed the cuboidal epithelium profusely covered with microvilli. SEM examinations of choroid plexus epithelium after chlordecone treatments revealed dose-dependent, cell alterations that effected the microvilli and cell surfaces. After the highest chlordecone dose, microvilli were no longer visible, the choroidal cell membrane appeared either smooth or pitted and there was evidence of increased luminal debris. TEM observations of the same choroid plexus cells revealed vacuolated cytoplasm, dilated endoplasmic reticulum, vacuolated mitochondria with disrupted cristae and cellular degeneration. SEM and TEM examination of choroid plexus after estradiol treatments revealed similar cellular alterations to those recorded after chlordecone treatments. The possible significance of these data are discussed.
J M
JM
Schumacher
V P
VP
Eroschenko
D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D007631:Chlordecone / Q000633:toxicity*; D002831:Choroid Plexus / Q000187:drug effects / Q000648:ultrastructure*; D004848:Epithelium / Q000648:ultrastructure; D004958:Estradiol / Q000494:pharmacology; D007306:Insecticides / Q000633:toxicity*; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D013499:Surface Properties; D014202:Tremor / Q000139:chemically induced
D007306:Insecticides; D004958:Estradiol; D007631:Chlordecone