31 phosphorous nuclear magnetic resonance spectroscopy of rat brain with temporary global cerebral ischemia--methodology.
31 P Magnetic Resonance Spectroscopy offers a technique for measuring intracellular pH and the high energy phosphates Phosphocreatine (PCr) and ATP in vivo. The 2-vessel occlusion model of temporary global cerebral ischaemia in the rat was adopted in this technique. This approach gave reliable results for intracellular pH and valuable information about PCr and ATP with an accurate time resolution for the changes during ischaemia and early reperfusion. The method may be a useful tool to evaluate possible benefits from different kinds of pre- and post ischaemic drug intervention.
Institute of Pharmacology and Toxicology, University of Trondheim, Norway.
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O
Haraldseth
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T
Southon
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T
Grønås
P
P
Jynge
S E
SE
Gisvold
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G
Unsgård
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D006863:Hydrogen-Ion Concentration; D002546:Ischemic Attack, Transient / Q000378:metabolism* / Q000503:physiopathology; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D010725:Phosphocreatine / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D010725:Phosphocreatine; D000255:Adenosine Triphosphate