Transcript length heterogeneity at the small heat shock protein genes of Drosophila.
Expression of the small heat shock protein (hsp) genes can be induced in cultured Drosophila cells by high temperature shock and by exposure to physiological doses of the insect molting hormone ecdysterone. Northern blot analysis was performed in order to compare the size of small hsp transcripts synthesized in response to these two stimuli. Transcripts from several other genes were also examined. Two types of length heterogeneity were observed for the small hsp gene transcripts. One involved the synthesis of what are designated as long form transcripts during heat shock; small hsp messenger RNAs extended at the 3' end by some 1.5 X 10(3) base-pairs. The second type of size heterogeneity observed is based on differences in the length of the poly(A) tail. The results of S1 nuclease protection analysis provided evidence that different initiation sites are not used for hsp 22 mRNA transcription in response to the two stimuli.
E M
EM
Berger
M P
MP
Vitek
C M
CM
Morganelli
D000818:Animals; D001483:Base Sequence; D003513:Cycloheximide / Q000494:pharmacology; D004330:Drosophila; D004441:Ecdysterone / Q000494:pharmacology; D005796:Genes; D006360:Heat-Shock Proteins / Q000235:genetics*; D006358:Hot Temperature; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D014158:Transcription, Genetic / Q000187:drug effects*
D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D004441:Ecdysterone; D012313:RNA; D003513:Cycloheximide