Immunohistochemical demonstration of T-lymphocyte subsets in tissue sections using monoclonal antibodies: a 3-layer PAP system and comparison of three immunoperoxidase methods.
Several immunohistochemical systems have been described to study T cells and their subsets in cryostat sections using monoclonal antibodies. In this report tonsils were studied by 2-layer and 4-layer immunoperoxidase techniques. In addition a 3-layer immunoperoxidase method using mouse PAP is proposed. The 2-layer technique proved unsatisfactory due to insufficient sensitivity. The other methods were characterized by comparably high sensitivity. The mouse PAP 3-layer system gave the most specific results due in particular to the complete absence of immunoglobulin staining in the germinal centers.
Jothy
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Boelcskei
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D000911:Antibodies, Monoclonal; D005069:Evaluation Studies as Topic; D006651:Histocytochemistry; D006801:Humans; D007120:Immunochemistry; D007124:Immunoenzyme Techniques / Q000592:standards; D014066:Palatine Tonsil / Q000473:pathology; D013194:Staining and Labeling; D013601:T-Lymphocytes / Q000145:classification*
D000911:Antibodies, Monoclonal