Use of nonfat dry milk to block nonspecific nuclear and membrane staining by avidin conjugates.
The use of fluorescein-avidin or rhodamine-avidin conjugates in conjunction with biotinylated secondary antibodies for indirect immunohistology frequently results in pronounced nonspecific nuclear staining in kidney sections. This nonspecific nuclear staining can be effectively blocked by using 5% (w/v) nonfat dry milk in buffered saline as the diluent for the avidin conjugates. In contrast, 5% (w/v) bovine serum albumin, a commonly used blocking agent, has only a modest effect. Nonfat dry milk is also effective as a blocking agent and carrier when used in fluorescence-activated cell sorter (FACS) analysis. These results emphasize the broad usefulness of milk-based blocking reagents.
R C
RC
Duhamel
D A
DA
Johnson
D000818:Animals; D001360:Avidin; D002462:Cell Membrane / Q000648:ultrastructure*; D002467:Cell Nucleus / Q000648:ultrastructure*; D005434:Flow Cytometry; D019793:Fluorescein; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D005526:Food, Formulated; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D007668:Kidney / Q000648:ultrastructure; D051379:Mice; D008892:Milk; D012235:Rhodamines; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013194:Staining and Labeling; D013950:Thymus Gland / Q000166:cytology
D005452:Fluoresceins; D012235:Rhodamines; D001360:Avidin; D012710:Serum Albumin, Bovine; D019793:Fluorescein