Antibody and inhibitor of chymase inhibit histamine release in immunoglobulin E-activated mast cells.
The low-molecular-weight inhibitor of chymase, chymostatin, and F(ab')2 fragments of anti-chymase markedly inhibited histamine release induced by anti-rat immunoglobulin E (IgE) but not that induced by compound 48/80. Inhibitors with molecular weights of more than 6,000, such as alpha 1-antichymotrypsin and aprotinin, and non-immunized F(ab')2 had no effect on histamine release. These results suggest that chymase in mast cell granules plays an essential role in the process of IgE-mediated degranulation. After degranulation, released chymase was associated with the cell surface while released tryptase was present in the extracellular milieu as a complex with a protein associated with tryptase (trypstatin).
H
H
Kido
N
N
Fukusen
N
N
Katunuma
D000818:Animals; D007611:Aprotinin / Q000494:pharmacology; D002918:Chymotrypsin / Q000037:antagonists & inhibitors / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects*; D007073:Immunoglobulin E / Q000276:immunology*; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007930:Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D007976:Leupeptins / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects* / Q000502:physiology; D009842:Oligopeptides / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D000514:alpha 1-Antichymotrypsin; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D007140:Immunoglobulin Fab Fragments; D007976:Leupeptins; D009842:Oligopeptides; D000514:alpha 1-Antichymotrypsin; D007073:Immunoglobulin E; D003189:p-Methoxy-N-methylphenethylamine; C002101:chymostatin; D007611:Aprotinin; D002918:Chymotrypsin; D007930:Leucine; C024974:E 64