Neuropeptides in the gastrointestinal canal of Necturus maculosus. Distribution and effects on motility.
The presence and distribution of regulatory peptides in nerves and endocrine cells of the stomach, intestine and rectum of a urodele amphibian, the mudpuppy, Necturus maculosus, was studied immunohistochemically in sections or whole-mount preparations of the gut wall. The effect of the occurring peptides on gut motility was studied in isolated strip preparations of circular and longitudinal smooth muscle from different parts of the gut. Bombesin-, neurotensin-, substance P- and VIP-like immunoreactivity was present in abundant nerve fibres in the myenteric plexus of both stomach, intestine and rectum. Single fibres or bundles were present in the circular muscle layer and in a well-developed deep muscular plexus in the intestine and rectum. Immunoreactive nerve cells were found in the myenteric plexus of the stomach, intestine (neurotensin only) and rectum. Gastrin/CCK-like immunoreactivity was observed only in a few fibres in stomach and rectum. Endocrine cells containing bombesin-, met-enkephalin-, gastrin/CCK-, neurotensin-, somatostatin- or substance P- like immunoreactivity were present in the mucosa. The effect of bombesin was an inhibition of the rhythmic activity in circular muscle preparations and in longitudinal muscle from the rectum, while longitudinal muscle from the stomach usually responded with a weak increase in tonus. Neurotensin, like-bombesin, was inhibitory on the spontaneous rhythmic activity of circular muscle throughout the gut, while the effect on longitudinal muscle was an increase in tonus. Met-enkephalin and substance P increased the tonus of all types of preparations, and often, in addition, initiated a rhythmic activity superimposed on this maintained tonus. VIP had a general inhibitory effect on the preparations, decreasing tonus and/or abolishing rhythmic activity. It is concluded that bombesin-, neurotensin-, substance P- and VIP-like peptides are present in nerves throughout the urodele gut and may have physiological functions in regulating the motility of the gut. The gastrin/CCK-like peptide present in nerves of the stomach and rectum may affect the function of these parts of the gut. The regulatory peptides present in endocrine cells may, perhaps with the exception of the somatostatin-like peptide, affect the motility humorally.
Holmgren
S
S
Jensen
J
J
Jönsson
A C
AC
Lundin
K
K
Nilsson
S
S
D000818:Animals; D001839:Bombesin / Q000032:analysis; D002766:Cholecystokinin / Q000032:analysis; D004064:Digestive System / Q000166:cytology* / Q000648:ultrastructure; D004068:Digestive System Physiological Phenomena; D004744:Enkephalin, Methionine / Q000032:analysis; D005455:Fluorescent Antibody Technique; D005755:Gastrins / Q000032:analysis; D005769:Gastrointestinal Motility; D007106:Immune Sera; D008854:Microscopy, Electron; D009337:Necturus; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009496:Neurotensin / Q000032:analysis; D013004:Somatostatin / Q000032:analysis; D013373:Substance P / Q000032:analysis; D014660:Vasoactive Intestinal Peptide / Q000032:analysis
D005755:Gastrins; D007106:Immune Sera; D009419:Nerve Tissue Proteins; D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D009496:Neurotensin; D013004:Somatostatin; D004744:Enkephalin, Methionine; D002766:Cholecystokinin; D001839:Bombesin