Axonal transport of substance P-hydrolyzing peptidase in rat sciatic nerves.
The axonal transport of substance P-hydrolyzing peptidase was studied both 2 and 10 days after the ligation of rat sciatic nerves. A peptidase(s) hydrolyzing substance P at the bonds of Phe7-Phe8 and Phe8-Gly9 was found to have accumulated to about 2 times the normal amount in the proximal segment 10 days after ligation. This enzyme activity was inhibited by ethylenediamine tetraacetate or dithiothreitol. These results suggest that this is a metalloendopeptidase which is slowly transported to inactivate neuropeptides in the nerve terminals.
Kato
T
T
Ishihara
H
H
Shimizu
A
A
D000818:Animals; D001370:Axonal Transport; D010450:Endopeptidases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008297:Male; D008666:Metalloendopeptidases; D009409:Nerve Crush; D051381:Rats; D011919:Rats, Inbred Strains; D012584:Sciatic Nerve / Q000201:enzymology* / Q000378:metabolism; D013373:Substance P / Q000378:metabolism*; D013997:Time Factors
D013373:Substance P; D010450:Endopeptidases; D008666:Metalloendopeptidases; C029610:substance P degrading enzyme