Patch-clamp technique in renal physiology.
The patch-clamp technique of Neher and Sakmann and their colleagues has been widely used over the last 5 years to investigate ion channels in excitable tissues. More recently, it has become useful as a tool to study channels involved in transepithelial ion transport. In this review, I briefly cover the basic concepts behind the patch-clamp technique and the kinds of information that can be obtained with it. I then summarize the applications of the technique to renal tissues and describe some of the channel types that have been observed to date in epithelia.
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Palmer
D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D004553:Electric Conductivity; D004848:Epithelium / Q000502:physiology; D005069:Evaluation Studies as Topic; D007473:Ion Channels / Q000378:metabolism / Q000502:physiology; D007668:Kidney / Q000502:physiology*; D010827:Physiology / Q000379:methods* / Q000639:trends; D014018:Tissue Distribution
D007473:Ion Channels