Azidocytidine is incorporated into RNA of 3T6 mouse fibroblasts.
Earlier work has shown that azidocytidine inhibits the growth and DNA synthesis of 3T6 mouse fibroblasts by inactivation of the enzyme ribonucleotide reductase. RNA synthesis, as measured by incorporation of [3H]cytidine was not affected. Here I show that azidocytidine is incorporated into RNA, but not into DNA. Incorporation of the analogue into RNA may under special circumstances contribute to the biological effect of the nucleoside.
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Akerblom
D000818:Animals; D002499:Centrifugation, Density Gradient; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003562:Cytidine / Q000031:analogs & derivatives* / Q000378:metabolism; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects; D005347:Fibroblasts / Q000378:metabolism; D051379:Mice; D012313:RNA / Q000302:isolation & purification / Q000378:metabolism*; D012264:Ribonucleotide Reductases / Q000037:antagonists & inhibitors
C015010:2'-azidocytidine; D003562:Cytidine; D012313:RNA; D004247:DNA; D012264:Ribonucleotide Reductases