A low molecular weight proteinase inhibitor produced by T lymphocytes.
A low molecular weight (MW) proteinase inhibitor, between 6500 and 21,500 MW, appeared in the supernatant of rabbit spleen cells cultured at high density for 24 hr. The inhibitor inhibited the enzymatic activity of trypsin for both a high MW natural substrate, fibrinogen, and for a low MW artificial substrate, Chromozym TRY. The low MW proteinase inhibitor is protein in nature and is different, in terms of specificity for enzymes, MW and sensitivity to different physical or chemical treatments, from aprotinin, a low MW proteinase inhibitor (6500 MW) of bovine origin, and from the soybean trypsin inhibitor, a relatively high MW proteinase inhibitor (21,500 MW). The inhibitor was found in the supernatant of purified T cells but not B cells, and its production was increased in the presence of an optimal concentration of Con A. The possibility that this proteinase inhibitor has a role in the regulation of trypsin-like proteinases involved to the immune response remains to be investigated.
Ganea
D
D
Teodorescu
M
M
Dray
S
S
D000818:Animals; D007611:Aprotinin / Q000494:pharmacology; D002470:Cell Survival; D002478:Cells, Cultured; D002863:Chromogenic Compounds / Q000378:metabolism; D003208:Concanavalin A / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005340:Fibrinogen / Q000378:metabolism; D007958:Leukocyte Count; D008970:Molecular Weight; D011480:Protease Inhibitors / Q000378:metabolism*; D011817:Rabbits; D013154:Spleen / Q000201:enzymology; D013601:T-Lymphocytes / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000378:metabolism
D002863:Chromogenic Compounds; D011480:Protease Inhibitors; D014361:Trypsin Inhibitors; D003208:Concanavalin A; D005340:Fibrinogen; D007611:Aprotinin; D014357:Trypsin