Oxygen as an antibiotic. The effect of inspired oxygen on bacterial clearance.
Previous experimentation with the guinea pig skin injection model showed that altering the fraction of inspired oxygen had a significant effect on infectious necrosis. Using the same model, we performed quantitative bacterial cultures to determine the number of viable injected bacteria 24 and 48 hours after injection. Animals were randomized to receive 12%, 21%, and 45% inspired oxygen. A significant decrease in bacterial number was seen at 45% inspired oxygen between 24 and 48 hours, and a significant decrease occurred at 48 hours between 12% and 45% inspired oxygen. These results demonstrated a prominent role for oxygen in bacterial clearance and host defense.
Department of Surgery, University of Minnesota, Minneapolis 55455.
D R
DR
Knighton
V D
VD
Fiegel
T
T
Halverson
S
S
Schneider
T
T
Brown
C L
CL
Wells
D000280:Administration, Inhalation; D000818:Animals; D000900:Anti-Bacterial Agents; D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology; D005260:Female; D006168:Guinea Pigs; D009336:Necrosis; D010100:Oxygen / Q000008:administration & dosage / Q000627:therapeutic use*; D012874:Skin Diseases, Infectious / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology
D000900:Anti-Bacterial Agents; D010100:Oxygen