Cytotoxin and enterotoxin production as factors delineating enteropathogenicity of Aeromonas caviae.
Twenty-one stool and environmental Aeromonas caviae isolates were studied for cytopathogenicity for cultured HEp-2 cells. Cytotoxic activity was demonstrated by the 21 A. caviae strains and by A. caviae ATCC 15468 after challenging HEp-2 cells with filtrates from a 24-h-old broth shake culture composed of double-strength, glucose-free Trypticase soy broth, incubated at 35 degrees C. Heat-stable cytotoxicity was observed 5 h after the addition of 1/5 and 1/10 concentrations of filtrates and consisted of cell rounding and cell detachment. Enterotoxin activity was also demonstrated through the suckling mouse assay. These data advance the role of A. caviae as a gastrointestinal pathogen.
Namdari
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Clinical Microbiology Laboratories, Mount Sinai Hospital, New York, New York 10029-6574.
Bottone
E J
EJ
D000333:Aeromonas / Q000378:metabolism* / Q000472:pathogenicity; D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D003603:Cytotoxins / Q000096:biosynthesis*; D004756:Enterobacteriaceae Infections / Q000209:etiology; D004768:Enterotoxins / Q000096:biosynthesis*; D006801:Humans; D051379:Mice; D014774:Virulence
D003603:Cytotoxins; D004768:Enterotoxins