HPLC analysis of diuron and metabolites in blood and urine.
A high-pressure liquid chromatographic method for the determination of diuron and its metabolites in human urine and blood is presented. The synthesis of different metabolites and of a suitable internal standard is described and the structure of the compounds is determined by mass spectrometry and nuclear magnetic resonance spectroscopy. The method is applied to an overdose case.
Catholic University of Louvain, Laboratory of Toxicology and Bromatology, Belgium.
M
M
Van Boven
L
L
Laruelle
P
P
Daenens
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D004237:Diuron / Q000378:metabolism* / Q000506:poisoning; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D012015:Reference Standards; D013055:Spectrophotometry, Infrared
D004237:Diuron