Rabbit antibodies with specificity for tyrosine phosphate are not reactive with tyrosine sulphate.
Tyrosine phosphate is a frequently observed modification in a limited number of protein molecules, principally the products of viral oncogenes and membrane receptors for mitogenic growth factors. Such a group is therefore an attractive candidate for affinity selection of such phosphoproteins by antibodies with specificity for tyrosine phosphate. This report demonstrates that antisera raised against 4-aminobenzylphosphonic acid have specificity for tyrosine phosphate, but are not inhibited by tyrosine sulphate. This observation has important ramifications for the detection and isolation of phosphotyrosine-containing proteins without copurification or detection of tyrosine sulphate-containing proteins, which are present in cells of all lineages. Furthermore, we demonstrate that antisera raised against the sulphanilate moiety are not reactive with tyrosine sulphate.
J A
JA
Jones
W
W
Cushley
D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D004797:Enzyme-Linked Immunosorbent Assay; D007106:Immune Sera; D007700:Kinetics; D019000:Phosphotyrosine; D011817:Rabbits / Q000276:immunology; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology
D000906:Antibodies; D000936:Antigen-Antibody Complex; D007106:Immune Sera; D019000:Phosphotyrosine; C005662:tyrosine O-sulfate; D014443:Tyrosine