The inhibition mechanism of histamine release by N-(3,4-dimethoxycinnamoyl) anthranilic acid.
N-(3,4-Dimethoxycinnamoyl) anthranilic acid (N-5') is an inhibitor of IgE-mediated histamine release from mast cells. To elucidate inhibition mechanism, effects of N-5' were examined under various conditions using peritoneal exudate cells and isolated mast cells of rats. N-5' inhibited histamine release induced by antigen, ionophore A23187, ATP, dextran and phospholipase A2. But the release induced by compound 48/80 or ionophore X537A was not inhibited. Influx of Ca++ into mast cells and ATP consumption were inhibited. Based on these results, it is presumed that N-5' interferes with the energy-requiring system and/or Ca++ influx resulting in the inhibition of histamine release.
A
A
Koda
Y
Y
Kurashina
M
M
Nakazawa
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D001202:Ascitic Fluid / Q000473:pathology; D002118:Calcium / Q000378:metabolism; D006636:Histamine Release / Q000187:drug effects*; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D062367:ortho-Aminobenzoates / Q000494:pharmacology*
D000227:Adenine Nucleotides; D062367:ortho-Aminobenzoates; C012293:tranilast; D002118:Calcium