Biological and immunological properties of haemagglutinin and neuraminidase expressed from cloned cDNAs in prokaryotic and eukaryotic cells.
To study the biological and immunological properties of influenza virus surface glycoproteins, cDNA copies of the haemagglutinin (HA) and the neuraminidase (NA) genes of A/WSN/33 influenza virus were cloned and expressed in prokaryotic and eukaryotic cells. In Escherichia coli, maximum expression of HA is obtained only as a fusion protein in which the NH2-terminal portion is provided by a bacterial protein (i.e. beta gal or trpLE'). The HA expressed in bacteria (bacterial HA) is recognized by polyclonal anti-WSN antibodies but not by neutralizing monoclonal antibodies. The antibodies made against the bacterial HA bind to the detergent-treated viral HA, intact virus and live influenza infected cells, but fail to show either haemagglutination inhibition (HI) or virus neutralization. These results suggest that the three-dimensional structure as well as the antigenic epitopes of the bacterial HA are different from that of native viral HA. HA, expressed from cDNA in cultured animal cells, is shown to possess the structural features of the native viral HA. It is glycosylated, transported to the apical domain of the plasma membrane of polarized cells, causes haemadsorption and can induce cell to cell fusion at low pH after proteolytic cleavage. An attempt was made to define the structural features of HA required for sorting and directional transport by making chimeras with vesicular stomatitis virus G (VSV G) proteins either by switching the amino terminus or the carboxy terminus of HA with that of VSV G. These chimeric proteins were translocated across the rough endoplasmic reticulum (RER) but were blocked in transport between the RER and cell membrane.(ABSTRACT TRUNCATED AT 250 WORDS)
D P
DP
Nayak
A R
AR
Davis
N L
NL
McQueen
T J
TJ
Bos
M A
MA
Jabbar
N
N
Sivasubramanian
G
G
Lionelli
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology; D005057:Eukaryotic Cells / Q000201:enzymology / Q000276:immunology; D006389:Hemagglutinins, Viral / Q000235:genetics* / Q000276:immunology; D009980:Influenza A virus / Q000201:enzymology / Q000235:genetics / Q000276:immunology; D009439:Neuraminidase / Q000235:genetics* / Q000276:immunology; D011387:Prokaryotic Cells / Q000201:enzymology / Q000276:immunology; D011487:Protein Conformation; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000276:immunology; D013329:Structure-Activity Relationship
D000956:Antigens, Viral; D000939:Epitopes; D006389:Hemagglutinins, Viral; D004247:DNA; D009439:Neuraminidase