Crystallization and preliminary x-ray investigation of recombinant human granulocyte-macrophage colony-stimulating factor.
Crystals of recombinant human granulocyte-macrophage colony-stimulating factor have been grown from solutions of polyethylene glycol 8000. The crystals are orthorhombic, space group P2(1)2(1)2(1); the axes are a = 45.5(1), b = 58.7(1) and c = 127.3(1) A. The crystals are stable to x-rays for at least 3 days and diffract beyond 2.8-A resolution. Although the molecule exists as a monomer in solution, it crystallizes with two or three molecules in the asymmetric unit.
Reichert
P
P
Schering Corporation, Bloomfield, New Jersey 07003.
Cook
W J
WJ
Ealick
S E
SE
Hammond
G S
GS
Le
H V
HV
Nagabhushan
T L
TL
Trotta
P P
PP
Bugg
C E
CE
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003115:Colony-Stimulating Factors; D003460:Crystallization; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances; D006801:Humans; D008970:Molecular Weight; D011994:Recombinant Proteins; D014961:X-Ray Diffraction
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor