[Differences in the expression of prekeratin and vimentin in organ and monolayer cultures of rat hepatocytes].
Results obtained by the indirect immunofluorescence method employing specific monoclonal antibodies show that during the first 24 hours of cultivation in a monolayer there appears another protein of intermediate filaments--vimentin, which is a characteristic of most mesenchymal cells. At the same time, in the organ liver culture maintained in the same culture medium, no expression of vimentin was observed up to 5-7 days of cultivation. Vimentin was revealed only in cells that migrated from a tissuepiece to collagen. Besides the vimentin expression in these migrating cells and monolayer cultures of hepatocytes, a redistribution of prekeratin filaments took place: the cytoplasmic network appeared instead of thick fibers underlying membranes. The results of the present work suggest that the vimentin expression and the prekeratin filament redistribution in epithelial liver cells in vitro do not depend on the changes of natural humoral factors for the components of culture medium but are due to damages of the intact liver tissue structure.
I D
ID
Karavanova
G A
GA
Bannikov
S M
SM
Troianovskiĭ
D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal / Q000032:analysis; D002478:Cells, Cultured; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D007381:Intermediate Filament Proteins / Q000032:analysis / Q000378:metabolism*; D007633:Keratins / Q000032:analysis / Q000378:metabolism*; D008099:Liver / Q000276:immunology / Q000378:metabolism*; D009924:Organ Culture Techniques; D011498:Protein Precursors / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D013997:Time Factors; D014746:Vimentin / Q000032:analysis / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D007381:Intermediate Filament Proteins; D011498:Protein Precursors; D014746:Vimentin; C004818:prekeratin; D007633:Keratins
Razlichiia ékspressii prekeratina i vimentina v organnykh i monosloĭnykh kul'turakh gepatotsitov krys.