Improved high-speed counter-current chromatograph with three multilayer coils connected in series. II. Separation of various biological samples with a semi-preparative column.
The semipreparative capability of the newly developed high-speed counter-current chromatograph equipped with a set of three multilayer coils has been demonstrated in separations of a variety of biological samples including triterpenoic acids, indole auxins, bacitracin, flavonoids and tetracycline derivatives, each with a suitable two-phase solvent system. The sample quantities ranging from 50 to 500 mg were efficiently separated within a few hours. The separation of tetracycline derivatives was remarkably improved by adding ammonium acetate to the solvent system.
Laboratory of Technical Development, National Heart, Lung, and Blood Institute, Bethesda, MD 20892.
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D001414:Bacitracin / Q000302:isolation & purification; D002845:Chromatography / Q000295:instrumentation* / Q000379:methods; D007210:Indoleacetic Acids / Q000302:isolation & purification; D010946:Plants, Medicinal; D010947:Plants, Toxic; D031955:Rhamnus / Q000378:metabolism; D013754:Tetracyclines / Q000302:isolation & purification
D007210:Indoleacetic Acids; D013754:Tetracyclines; D001414:Bacitracin