Monoclonal antibodies to digoxin: comparison of in vitro and in vivo immunization.
We compared the primary response in vitro with the secondary response in vivo of A/J inbred and CD-1 outbred mice to digoxin-HSA. The frequencies of hybrid formation and growth, and of the hybridomas that secreted antibody to digoxin were similar in both strains regardless of the immunization procedure. The patterns of cross-reactivity of the monoclonal antibodies to two compounds structurally related to digoxin (ouabain and digitoxin) were likewise similar for both strains and immunization procedures.
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Buchman
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Miller
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Koch
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002301:Cardiac Glycosides / Q000276:immunology; D002478:Cells, Cultured; D003429:Cross Reactions; D004077:Digoxin / Q000276:immunology*; D000939:Epitopes; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D007156:Immunologic Memory; D051379:Mice
D000911:Antibodies, Monoclonal; D002301:Cardiac Glycosides; D000939:Epitopes; D004077:Digoxin