Platelet-activating factor-induced aggregation of rat alveolar macrophages.
Isolated rat alveolar macrophages aggregate in the presence of PAF in a dose- and cell-dependent manner. Saturation was achieved at 80 microM PAF. The response was increased linearly with cell number up to a concentration of 3 x 10(6) cells/ml, but decreased at higher cell concentrations. The stereoisomer, enantio-PAF, and the C2-acetyl hydrolyzed product, lyso-PAF, each caused aggregation of isolated rat alveolar macrophages in a manner similar to that for PAF. The PAF-induced aggregation of alveolar macrophages may be mediated through non-specific binding sites and may represent a toxic response to relatively high levels of PAF that released at localized sites.
National Institute for Occupational Safety and Health, Division of Respiratory Disease Studies, Morgantown, WV 26505.
J
J
Rabovsky
M
M
Goddard
W H
WH
Pailes
D J
DJ
Judy
V
V
Castranova
D000818:Animals; D002449:Cell Aggregation / Q000187:drug effects*; D066298:In Vitro Techniques; D008264:Macrophages / Q000187:drug effects*; D008297:Male; D010972:Platelet Activating Factor / Q000031:analogs & derivatives / Q000494:pharmacology*; D011650:Pulmonary Alveoli / Q000166:cytology / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D013237:Stereoisomerism
C029271:O-deacetyl platelet activating factor; D010972:Platelet Activating Factor