Regional [14C]palmitate incorporation into the hippocampus after transient cerebral ischemia in awake gerbils.
Incorporation of plasma [14C]palmitate into the hippocampus was measured using quantitative autoradiography and a ratio method of analysis between 10 min and 7 days after 5 min of bilateral occlusion of the common carotid arteries in awake gerbils. One day after carotid occlusion, relative palmitate incorporation was elevated by 16% and 20%, respectively (p less than 0.01), in the CA4 pyramidal cell layer and dentate gyrus of the hippocampus as compared with sham-operated animals. At 3 days, significant elevations of this magnitude were found in the CA3 and CA4 cell layers, and relative incorporation was reduced by 26% in the CA1 pyramidal cell layer. At 7 days, the only significant difference from control was a 15% elevated incorporation in the CA3 pyramidal cell layer. The results suggest that the turnover of palmitate-containing lipids is retained in the CA1 pyramidal layer of the gerbil hippocampus for 1 day and then reduced at 3 days after 5-min ischemia and that lipid synthesis is stimulated in hippocampal regions affected by but recovering from an ischemic insult (CA3, CA4, dentate gyrus).
Department of Neurosurgery, Tokyo Medical and Dental University, School of Medicine, Japan.
O
O
Tone
Y
Y
Matsushima
Y
Y
Inaba
S I
SI
Rapoport
D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology; D002470:Cell Survival; D005849:Gerbillinae; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D008297:Male; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism*; D013997:Time Factors; D014851:Wakefulness
D010169:Palmitic Acids; D019308:Palmitic Acid