Murine transfer factor. III. Specific interactions between transfer factor and antigen.
The interactions between dialyzable transfer factor and antigens have been studied. Incubation of transfer factor-containing dialysates from ferritin-sensitized mice or ferritin-coated plastic surfaces removed the antigen-sensitizing activity; incubations of the same preparations on cytochrome c-coated surfaces did not. Similar results were obtained when cytochrome c-transfer factor was studied. Incubation on cytochrome c-coated surfaces removed the activity, but incubation on ferritin-coated surfaces did not. Specific transfer factor activities could be recovered by elution with 8 M urea or acetonitrile. The finding of interactions between transfer factor and antigens provides evidence for a molecular basis of the specificity of the immunologic effects of transfer factor. This technique may also enable us to obtain amounts of specific material that are adequate for chemical analysis.
C H
CH
Kirkpatrick
S J
SJ
Rozzo
J J
JJ
Mascali
D000818:Animals; D000941:Antigens / Q000032:analysis / Q000276:immunology*; D002417:Cattle; D010856:Columbidae; D003574:Cytochrome c Group / Q000276:immunology / Q000378:metabolism; D000939:Epitopes; D005260:Female; D005293:Ferritins / Q000276:immunology / Q000378:metabolism; D006736:Horses; D006968:Hypersensitivity, Delayed / Q000378:metabolism; D007163:Immunosorbent Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D011485:Protein Binding; D013154:Spleen / Q000166:cytology; D014165:Transfer Factor / Q000032:analysis / Q000276:immunology* / Q000378:metabolism
D000941:Antigens; D003574:Cytochrome c Group; D000939:Epitopes; D014165:Transfer Factor; D005293:Ferritins