The histones of the insect trypanosomatid, Crithidia fasciculata.
The histone-like proteins of the monogenetic parasite Crithidia fasciculata were extracted with 0.2 M sulfuric acid either from purified nuclei, or from purified chromatin, in both cases in the presence of 1 mM tosyl lysylchloromethylketone and 2 mM phenyl methyl sulfonyl fluoride as proteinase inhibitors. The presence of histones in the flagellate, nonidentical with those from calf thymus used as controls, was shown by their electrophoretic patterns in three different polyacrylamide gel systems; their staining with Alkaline fast green, specific for basic proteins; their global amino acid composition and absorption spectrum and their molecular weights. The protein showing the slower mobility in SDS gels and the fastest mobility in the urea-acetic acid-Triton gels, seems to be an H1 histone, because of its metachromatic staining with Coomassie brilliant blue, solubility characteristics, differential destaining properties and amino acid composition. Band 5 in Triton-urea-acetic acid gels is probably an HMG protein. We conclude that C. fasciculata has a complete set of histones and that the lack of chromosome condensation during mitosis is not due to lack of histone H1.
Instituto de Investigaciones Bioquímicas Fundación Campomar, Universidad de Buenos Aires, CONICET, Argentina.
V G
VG
Duschak
J J
JJ
Cazzulo
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000032:analysis / Q000648:ultrastructure; D002843:Chromatin / Q000032:analysis / Q000648:ultrastructure; D003421:Crithidia / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000302:isolation & purification*; D007202:Indicators and Reagents; D008970:Molecular Weight; D013045:Species Specificity; D013950:Thymus Gland / Q000032:analysis
D000596:Amino Acids; D002843:Chromatin; D006657:Histones; D007202:Indicators and Reagents