A chymotrypsin-type serine protease in rat basophilic leukemia cells: evidence for its immunologic identity with atypical mast cell protease.
Activity of a chymotrypsin-type serine protease was found in a subline of rat basophilic leukemia (RBL-2H3) cells. The protease was immunologically cross-reactive with anti-atypical mast cell protease immunoglobulin (Ig) G, and its activity was inhibited in a dose-dependent manner by the antibody. The apparent m.w. of the protease that reacted with the antibody was 25,000, which was identical with that of atypical mast cell protease in rat mucosal mast cells. These results show that the chymotrypsin type serine protease in RBL-2H3 cells is immunologically identical with atypical mast cell protease, which was first purified from rat small intestine. Immunohistochemical studies showed that the protease was located not only in intracytoplasmic granules but also in organelles synthesizing protein, such as cisternae of the rough endoplasmic reticulum, perinuclear spaces, and the Golgi apparatus. However, no immunoreactivity was demonstrated in rat basophils. The activity of the protease increased in the exponential phase of growth of RBL-2H3 cells in which some activity was also detected in the medium, and it decreased in the late stationary phase.
H
H
Kido
K
K
Izumi
H
H
Otsuka
N
N
Fukusen
Y
Y
Kato
N
N
Katunuma
D000818:Animals; D001491:Basophils / Q000201:enzymology / Q000648:ultrastructure; D002460:Cell Line; D053818:Chymases; D002918:Chymotrypsin / Q000276:immunology*; D003429:Cross Reactions; D010450:Endopeptidases / Q000276:immunology*; D006651:Histocytochemistry; D007074:Immunoglobulin G; D007938:Leukemia / Q000201:enzymology* / Q000473:pathology; D008297:Male; D008407:Mast Cells / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D012697:Serine Endopeptidases; D013194:Staining and Labeling
D007074:Immunoglobulin G; D010450:Endopeptidases; D012697:Serine Endopeptidases; D002918:Chymotrypsin; D053818:Chymases