Localization of two epitopes of protein L7/L12 to both the body and stalk of the large ribosomal subunit. Immune electron microscopy using monoclonal antibodies.
Four molecules of ribosomal protein L7/L12 are found as two dimers on the Escherichia coli 50 S ribosomal subunit. Immune electron microscopy using monoclonal antibodies directed against two epitopes of protein L7/L12 has allowed placement of elements of each dimer. One monoclonal antibody, directed against a determinant in the COOH-terminal domain, allows localization of two identical determinants at or near the end of the subunit stalk. The same antibody was used to place two additional determinants at the periphery of stalkless subunits, in an area from which a stalk might be expected to project. A second antibody, directed against an epitope in the amino-terminal portion of L7/L12, caused loss of stalks from the 50 S subunits. The micrographs showed symmetrical oligometric complexes of the dissociated dimeric protein with bivalent antibody. Antibodies were also seen to bind to the body of stalkless subunits, in a region near the COOH-terminal sites. The results are explained by a model in which one dimer of protein L7/L12 exists in a folded conformation on the subunit body and the second dimer occurs in an extended conformation in the subunit stalk.
H M
HM
Olson
A
A
Sommer
D S
DS
Tewari
R R
RR
Traut
D G
DG
Glitz
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins / Q000032:analysis*; D000939:Epitopes / Q000032:analysis*; D004926:Escherichia coli / Q000648:ultrastructure*; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D012269:Ribosomal Proteins / Q000032:analysis* / Q000276:immunology; D012270:Ribosomes / Q000648:ultrastructure*
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D000939:Epitopes; D046911:Macromolecular Substances; D012269:Ribosomal Proteins; C016700:ribosomal protein L7-L12