Proliferation of tartrate-resistant acid phosphatase positive multinucleate cells in ovariectomized animals.
In order to explore why ovarian hormone deficiency causes excessive osteoclastic bone resorption that results in osteoporosis in a large number of postmenopausal women, bone marrow cells from ovariectomized and sham-operated female mice were cultured for 8 days. The cells gave rise in culture to tartrate-resistant acid phosphatase-positive multinucleate cells. The formation of these osteoclast-like cells was enhanced by parathyroid hormone and 1,25(OH)2vitamin D3, with the latter being more effective. Cultures of cells from ovariectomized animals formed significantly more tartrate-resistant acid phosphatase-positive multinucleate cells than those from sham-operated controls. These findings support the hypothesis that ovarian hormone deficiency promotes the expansion of a pool of marrow-derived progenitor cells that differentiate into bone-resorbing osteoclasts under the influence of osteotropic hormones.
Department of Physiology, University of Texas Health Science Center, San Antonio 78284.
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D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells; D002117:Calcitriol / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D051379:Mice; D008813:Mice, Inbred ICR; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D010052:Ovariectomy; D010053:Ovary / Q000502:physiology*; D010281:Parathyroid Hormone / Q000494:pharmacology*; D013644:Tartrates / Q000494:pharmacology
D010281:Parathyroid Hormone; D013644:Tartrates; D000135:Acid Phosphatase; D002117:Calcitriol; C029768:tartaric acid