T15-specific helper T cells: analysis of idiotype specificity by competitive inhibition analysis.
Optimal activation of T15 idiotype-bearing B cells has been shown previously to be influenced by two subsets of Thy-1+, Ly-1+,2-sIg- helper T cells. One of the helper T cell sets appears to be T15 specific in that its presence results in a selective augmentation of T15-bearing anti-phosphorylcholine (PC) plaque-forming cell responses. To determine the precise specificity of the idiotype-specific helper T cell (ThId), Ly-1 T cells were tested in an in vitro anti-PC response for their ability to bind directly to T15 myeloma protein-coated plastic plates. Specificity of this binding was ascertained by competitive inhibition of plate binding using idiotypically related myeloma or hybridoma proteins. These data suggest that the Ly-1 T cells which augment T15-bearing plaque-forming cell responses can bind to T15-coated plates and are T15 idiotype specific. This approach is being used currently to attempt to clone ThId cells to further analyze their activation requirements and specificities.
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Becker Dunn
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D000818:Animals; D000917:Antibody Formation; D000950:Antigens, Ly; D001667:Binding, Competitive; D002448:Cell Adhesion; D002794:Choline / Q000031:analogs & derivatives*; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D006241:Haptens / Q000032:analysis / Q000276:immunology; D006462:Hemolytic Plaque Technique; D007130:Immunoglobulin Idiotypes / Q000032:analysis*; D008211:Lymphocyte Cooperation; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D010767:Phosphorylcholine / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000145:classification* / Q000276:immunology
D000950:Antigens, Ly; D000939:Epitopes; D006241:Haptens; D007130:Immunoglobulin Idiotypes; D010767:Phosphorylcholine; D010047:Ovalbumin; D002794:Choline