Cytolytic T cell hybridomas. III. The antigen specificity and the restriction specificity of cytolytic T cells do not phenotypically mix.
We have obtained cytolytic T-T hybrids by fusing an H-2Kk restricted clone specific for the hapten 3-(p-sulfophenyldiazo)-4-hydroxyphenyl acetic acid (SP) with an H-2Dd-restricted clone specific for the hapten fluorescein (FL). Several hybrid clones express both parental specificities but fail to lyse SP-coupled H-2Dd and FL-coupled H-2Kk target cells. We also fused the H-2Kk-restricted, SP-specific clone with a clone which recognizes FL in the context of any class I major histocompatibility complex antigen. Again several hybrids show both parental specificities but fail to recognize SP coupled to target cells which are not recognized by the parental SP-specific clone. These findings indicate that the observed cytotoxic T lymphocyte specificities for haptens on the one hand and polymorphic as well as nonpolymorphic class I major histocompatibility complex antigenic determinants on the other hand are not carried by independent proteins.
Haas
W
W
Mathur-Rochat
J
J
Kisielow
P
P
von Boehmer
H
H
D000818:Animals; D002459:Cell Fusion; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000276:immunology*; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins / Q000276:immunology; D006183:H-2 Antigens / Q000276:immunology*; D006241:Haptens / Q000276:immunology; D006825:Hybridomas / Q000145:classification* / Q000276:immunology; D051379:Mice; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D010641:Phenotype; D010648:Phenylacetates / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000145:classification* / Q000276:immunology; D013861:Thiocyanates / Q000276:immunology
D000939:Epitopes; D005452:Fluoresceins; D006183:H-2 Antigens; D006241:Haptens; D010648:Phenylacetates; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate