[Structural rearrangements induced by glycerol increase the permeability of bilayer lipid membranes for amphotericin].
It has been shown that structural rearrangements induced by glycerol in bilayer lipid membranes (BLM) containing cholesterol facilitate the transmembrane transport of amphotericin B molecules in the direction of glycerol gradient. The addition of amphotericin B to the same side with glycerol results in a change in bilayer selectivity from the cation to the anion one. Besides, the final conductivity is blocked by tetraethylammonium from the solution with no amphotericin B added. It testifies to the transport of amphotericin molecules to the opposite side of the membrane. The transport effect depends on the cholesterol content in bilayer, ionic strength of the medium and slightly depends on temperature. It is concluded that transport of amphotericin B in such conditions differs from the diffusive one and is due to the formation of intermediate lipid phases in the course of structural rearrangements of bilayers.
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Rudenko
D000666:Amphotericin B / Q000378:metabolism*; D002784:Cholesterol; D005990:Glycerol / Q000494:pharmacology*; D007473:Ion Channels / Q000187:drug effects; D008051:Lipid Bilayers; D008564:Membrane Potentials / Q000187:drug effects; D010539:Permeability
D007473:Ion Channels; D008051:Lipid Bilayers; D000666:Amphotericin B; D002784:Cholesterol; D005990:Glycerol
Pri strukturnykh perestroĭkakh, indutsirovannykh glitserinom, uvelichivaetsia pronitsaemost' bisloĭnykh lipidnykh membran dlia amfoteritsina.