The induction and characterization of rat liver stearyl-CoA desaturase mRNA.
Poly(A+) RNA isolated from livers of rats induced for stearyl-CoA desaturase contains elevated levels of mRNA for this enzyme which is translated in a rabbit reticulocyte system. The protein is immunologically and by peptide fingerprinting following Staphylococcus aureus V8 protease digestion identical to the isolated enzyme and, therefore, not synthesized in a detectable larger precursor form. The desaturase mRNA is selectively translated on free cytoplasmic polysomes from rat liver and represents at least a 40-fold increase in translatable mRNA in livers of induced animals. Northern blot analysis, using a cDNA probe complementary to rat liver desaturase mRNA, demonstrated that the desaturase is encoded by a 4900-base mRNA which is elevated approximately 50-fold in induced liver.
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D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004435:Eating; D004790:Enzyme Induction; D004926:Escherichia coli / Q000235:genetics; D044943:Fatty Acid Desaturases / Q000235:genetics*; D008099:Liver / Q000201:enzymology*; D008297:Male; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000235:genetics; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D013217:Starvation; D013230:Stearoyl-CoA Desaturase / Q000096:biosynthesis / Q000235:genetics*
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA; D044943:Fatty Acid Desaturases; D013230:Stearoyl-CoA Desaturase