Phorbol myristate acetate stimulates RNA and casein synthesis in cultured mouse mammary gland tissues.
Prolactin and phorbol myristate acetate (TPA) stimulate the rate of [3H]uridine incorporation in cultured mouse mammary gland explants in a similar fashion. Both the time-courses and magnitude of responses were the same; in addition, maximum stimulatory concentrations of TPA and prolactin elicited a nonadditive response when tested together. Nordihydroguaiaretic acid (NDGA), a lipoxygenase inhibitor, abolished both the TPA and prolactin effects on [3H]uridine incorporation. TPA also effected an enhanced rate of [3H]leucine incorporation into a casein-rich phosphoprotein fraction, but only if the explants were also cultured with spermidine.
J A
JA
Rillema
S B
SB
Waters
D000818:Animals; D002364:Caseins / Q000096:biosynthesis*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D008321:Mammary Glands, Animal / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D009955:Ornithine Decarboxylase / Q000378:metabolism; D010704:Phorbols / Q000494:pharmacology*; D011247:Pregnancy; D011388:Prolactin / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis*; D013095:Spermidine / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D010738:Type C Phospholipases / Q000378:metabolism
D002364:Caseins; D010704:Phorbols; D012313:RNA; D011388:Prolactin; D011493:Protein Kinase C; D010738:Type C Phospholipases; D009955:Ornithine Decarboxylase; D013755:Tetradecanoylphorbol Acetate; D013095:Spermidine