High-performance liquid chromatography of biogenic amines and metabolites in brain, cerebrospinal fluid, urine and plasma.
A method for high-performance liquid chromatographic separation and electrochemical detection of biogenic amines and metabolites in a variety of biological matrices is described. The method employs either homogenization, precipitation or dilution followed by direct injection of the samples and permits the chromatographic resolution of dopamine, norepinephrine, epinephrine, serotonin (5-HT), 3,4-dihydroxyphenylacetic acid (DOPAC), 5-hydroxyindoleacetic acid (5-HIAA) and homovanillic acid (HVA) in brain; 3-methoxy-4-hydroxyphenylglycol, DOPAC, 5-HIAA and HVA in cerebrospinal fluid; 5-HIAA, HVA and 5-HT in plasma; and 5-HIAA and HVA in urine. Alterations in chromatographic conditions, voltammetry and in vivo pharmacological manipulations are employed to verify the identity of the putative neurotransmitter and metabolite peaks in the biological samples.
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D000818:Animals; D001679:Biogenic Amines / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid / Q000652:urine; D001923:Brain Chemistry; D002421:Caudate Nucleus / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D006719:Homovanillic Acid / Q000097:blood; D006897:Hydroxyindoleacetic Acid / Q000097:blood; D007031:Hypothalamus / Q000032:analysis; D008254:Macaca nemestrina; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000097:blood
D001679:Biogenic Amines; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D006719:Homovanillic Acid