Enhanced activity of immobilized dimethylmaleic anhydride-protected poly- and monoclonal antibodies.
The effect of reversible protection of the free amino groups of poly- and monoclonal antibodies by dimethylmaleic anhydride on their binding activity following immobilization onto various carriers was studied. The treatment with dimethylmaleic anhydride resulted in a 1.6-1.8-fold increase in the activity of immobilized goat anti-mouse immunoglobulin antibody immobilized onto different epoxy containing carriers and a 3-10.7-fold increase in the activity of immobilized monoclonal antibodies specific for carboxypeptidase A. The increase in activity was most pronounced at low antigen to carrier loads and over a wide range of modifier to protein ratios. The application of reversible protection of antibodies may permit the development of highly active immobilized antibody preparations for use in immunoaffinity purification.
Hadas
E
E
Department of Biotechnology, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Israel.
Koppel
R
R
Schwartz
F
F
Raviv
O
O
Fleminger
G
G
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D008024:Ligands; D008299:Maleic Anhydrides; D051379:Mice; D008815:Mice, Inbred Strains; D012685:Sepharose
D000906:Antibodies; D000911:Antibodies, Monoclonal; D008024:Ligands; D008299:Maleic Anhydrides; C007474:2,3-dimethylmaleic anhydride; D012685:Sepharose