A procedure for long-term culture of oligodendrocytes.
A procedure for long-term culture of oligodendrocytes is described, the starting material being 20-day-old primary mixed cultures of newborn rat brain. Cells were first incubated in a serum-free medium for 48 h before they were subcultured on poly-L-lysine coated plastic dishes. After this treatment, the oligodendrocytes developed well in Waymouth medium containing 10% (v/v) calf serum, while most of the astrocytes died. At 13 days in subculture more than 90% of the cells were identified as oligodendrocytes; the criteria for oligodendrocytes were based on their immunoreactivity to antisera against W1 Wolfgram protein, myelin basic proteins and the synthetic C-terminal hexapeptide of the major myelin proteolipid. At 13 and 19 days astrocytes were present, 7% and 20% respectively. The culture system described here may be useful to study the biochemical and immunological aspects of the oligodendrocytes.
A
A
Espinosa de los Monteros
G
G
Roussel
J L
JL
Nussbaum
D000818:Animals; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods*; D007106:Immune Sera; D004676:Myelin Basic Protein / Q000032:analysis / Q000276:immunology; D009185:Myelin Proteins / Q000032:analysis / Q000276:immunology; D009457:Neuroglia / Q000166:cytology*; D009836:Oligodendroglia / Q000032:analysis / Q000145:classification / Q000166:cytology*; D051381:Rats; D013194:Staining and Labeling; D013997:Time Factors
D007106:Immune Sera; D004676:Myelin Basic Protein; D009185:Myelin Proteins; C028312:Wolfgram proteins