Effect of temperature on calcium exchange in digitonin-treated rat ventricular myocytes.
The effect of a change in temperature on net mitochondrial Ca2+ exchange has been investigated in a suspension of adult rat ventricular myocytes. Temperature was varied between 42 degrees C and 15 degrees C. Hypothermia reduced the initial rate of respiration-dependent Ca2+ uptake and reduced the Na+-sensitivity of Ca2+ efflux. The net result of these alterations is that at low temperatures, the Ca2+ level at which a steady-state between mitochondria and sarcoplasm is maintained, will be raised.
D
D
Harding
C
C
Fry
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003080:Cold Temperature; D004072:Digitonin / Q000494:pharmacology*; D006352:Heart Ventricles / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D008929:Mitochondria, Heart / Q000187:drug effects / Q000378:metabolism*; D010101:Oxygen Consumption; D051381:Rats; D012430:Ruthenium Red / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology
D012430:Ruthenium Red; D012964:Sodium; D004072:Digitonin; D002118:Calcium