Fine specificity analysis of lactate dehydrogenase B-specific proliferating T cell clones: implications for the mechanism of alloreactivity.
T cell clones of C57BL/6 origin which recognize porcine lactate dehydrogenase B (LDH-BP) together with the Ab molecule were characterized in terms of fine specificity for both LDH-B and self-major histocompatibility complex determinants. Using antigen-presenting cells from the Ab-mutant strain B6.C-H-2bm12 (bm12), three clonotypes could be distinguished: the first responds to LDH-BP + bm12, the second fails to respond and the third is alloreactive to bm12. The last clone exhibits additional alloreactivities to A molecules expressed in strains of H-2 haplotypes f, r, s, u, w6, w7, w16, w17 and w23. All three clonotypes give identical response patterns to a panel of 17 different dehydrogenase enzymes, and react to the same tryptic peptide of LDH-BP. Thus, these clones appear to recognize the same LDH-B epitope together with at least 3 different determinants of the Ab molecule. The data suggest that alloreactivity is more closely related to T cell specificity for self-major histocompatibility complex than to specificity for foreign antigen.
Z A
ZA
Nagy
C
C
Servis
P
P
Walden
J
J
Klein
E
E
Goldberg
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D002417:Cattle; D002999:Clone Cells; D004285:Dogs; D000939:Epitopes; D007519:Isoantigens / Q000276:immunology; D007770:L-Lactate Dehydrogenase / Q000276:immunology; D008291:Malate Dehydrogenase / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D011817:Rabbits; D013154:Spleen / Q000166:cytology; D013552:Swine; D013601:T-Lymphocytes / Q000201:enzymology*; D014337:Trout
D000939:Epitopes; D007519:Isoantigens; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase