Monoclonal antibodies against a beta-galactoside-binding lectin of chick embryo.
Monoclonal antibodies against an endogenous beta-galactoside-binding lectin (monomer molecular weight 14,000, 14K lectin) of chick embryo were prepared and characterized. The inhibitory activities against hemagglutination, antigenic determinants and binding specificities were examined. Monoclonal antibody S1A4-5 strongly inhibited the hemagglutination activity of this lectin. This antibody did not bind to any cyanogen bromide (BrCN) fragment of the lectin. Another monoclonal antibody, S1A4-3, bound to one of the BrCN fragments (residues 34-66). However, this antibody inhibited hemagglutination only weakly. The bindings to isolectins of beta-galactoside-binding lectin, namely 14K lectin (monomer molecular weight 16,000) and a third species which is assumed to be a hybrid molecule consisting of 14K and 16K lectin subunits, were examined. The antibody SIA4-5 bound to 14K lectin but not to 16K lectin. In the case of the third species, intermediate binding was observed.
Oda
Y
Y
Hirabayashi
J
J
Kasai
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000302:isolation & purification; D000918:Antibody Specificity; D001667:Binding, Competitive; D002642:Chick Embryo; D003488:Cyanogen Bromide / Q000378:metabolism / Q000494:pharmacology; D000939:Epitopes / Q000032:analysis; D037161:Galectins; D006386:Hemagglutination Tests; D006388:Hemagglutinins / Q000276:immunology* / Q000302:isolation & purification; D006825:Hybridomas / Q000378:metabolism; D007163:Immunosorbent Techniques; D037102:Lectins / Q000032:analysis; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D000939:Epitopes; D037161:Galectins; D006388:Hemagglutinins; D037102:Lectins; D003488:Cyanogen Bromide