Effect of 1-p-chlorobenzyl-1H-indazol-3-carboxylic acid (AF 1312/Ts) on the sex organs of the male laboratory mouse.
Oral administration (500 mg/kg body weight/day, for 28 days) of 1-p-chlorobenzyl-1H-indazol-3-carboxylic acid (AF 1312/TS) induced suppression of spermatogenesis without adversely affecting the Leydig cells. The seminiferous tubules were almost depopulated and contained only Sertoli cells and spermatogonia. The drug also induced significant decrease in the levels of testicular RNA and protein; DNA level, however, remained unaltered. Regressive histological changes were also noted in the epididymides of drug-treated mice. Cauda epididymidal spermatozoa from drug-treated mice were immotile and fragmented. AF 1312/TS caused no alteration in the level of fructose in the seminal vesicle. The drug-induced alterations were reversible, and 56 days after drug withdrawal the reproductive organs returned to their pretreatment state. The results suggest that AF 1312/TS selectively suppresses spermatogenesis without interfering with the endocrine functions of the testis.
Singh
S K
SK
Dominic
C J
CJ
D000818:Animals; D000988:Antispermatogenic Agents / Q000494:pharmacology*; D004822:Epididymis / Q000033:anatomy & histology / Q000187:drug effects; D005837:Genitalia, Male / Q000033:anatomy & histology / Q000187:drug effects*; D007191:Indazoles / Q000494:pharmacology*; D007985:Leydig Cells / Q000187:drug effects; D008297:Male; D051379:Mice; D011506:Proteins / Q000378:metabolism; D011720:Pyrazoles / Q000494:pharmacology*; D012313:RNA / Q000378:metabolism; D013091:Spermatogenesis / Q000187:drug effects; D013737:Testis / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
D000988:Antispermatogenic Agents; D007191:Indazoles; D011506:Proteins; D011720:Pyrazoles; C007762:1-p-chlorobenzyl-1H-indazole-3-carboxylic acid; D012313:RNA