Interleukin 1, but not interleukin 1 inhibitor, is released from human monocytes by immune complexes.
Immune complexes (IC) are believed to play a role in the pathogenesis of some autoimmune diseases in which interleukin 1 (IL-1) and probably other cytokines also take part. This investigation shows that tetanus toxoid-human anti-tetanus toxoid IC induce human monocytes to release IL-1. The activity was identified as being mainly IL-1 beta by molecular size chromatography, isoelectric focusing, and anti-IL-1 beta affinity chromatography. Endotoxins were eliminated by repetitive washing of the IC suspension and by preincubation of IC with polymyxin B. The IL-1-inducing effect of IC was destroyed by heating at 80 degrees C, and it was not blocked by the cytoskeleton inhibitor cytochalasin B. IL-1 inhibitors were not detected in the supernatants.
Department of Medicine TTA, Rigshospitalet, University of Copenhagen, Denmark.
L
L
Remvig
B S
BS
Thomsen
L
L
Baek
M
M
Svenson
K
K
Bendtzen
D000936:Antigen-Antibody Complex / Q000502:physiology*; D002845:Chromatography; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007375:Interleukin-1 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D008970:Molecular Weight; D009000:Monocytes / Q000378:metabolism*
D000936:Antigen-Antibody Complex; D007375:Interleukin-1