Class II antigens on canine T lymphocytes.
A panel of crossreactive anti-human, -mouse and -rat MHC class II monoclonal antibodies (Mabs) was used to examine MHC class II antigen expression on canine T lymphocytes by cytofluorometry. The presence of MHC class II antigens was demonstrated on activated T lymphoblasts as well as on non-stimulated peripheral blood T lymphocytes. A number of anti-MHC class II Mabs reacted only with activated T lymphoblasts. Immunoprecipitation studies confirmed the Ia-like or MHC class II molecular character of the antigens on canine T cells. The expression of MHC class II antigens on peripheral blood T lymphocytes appeared to be not induced by stimulation of the T cells, as purified T lymphocytes of specific pathogen free dogs reacted with anti-MHC class II Mabs. Moreover, the study indicates that MHC class II antigen expression is present in the neonatal thymus. Lectin stimulated and allogeneically stimulated T lymphoblasts showed a stronger expression of MHC class II antigens in comparison with non-stimulated T cells.
Doveren
R F
RF
Buurman
W A
WA
Schutte
B
B
Groenewegen
G
G
van der Linden
C J
CJ
D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal / Q000276:immunology; D004285:Dogs / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D006649:Histocompatibility Antigens / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D051379:Mice; D010835:Phytohemagglutinins / Q000494:pharmacology; D013047:Specific Pathogen-Free Organisms; D013601:T-Lymphocytes / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006649:Histocompatibility Antigens; D010835:Phytohemagglutinins