A monoclonal antibody-based immunoassay for human lactoferrin.
Monoclonal antibodies against human lactoferrin define at least 3 and possibly as many as 6 different epitopes. A sandwich enzyme-linked immunoassay, using monoclonals against different epitopes, has been optimised for the measurement of serum lactoferrin. In 35 samples from healthy adults the mean lactoferrin content of serum from blood clotted overnight was 0.54 +/- 0.26 micrograms/ml.
Chung
S
S
Hayward
C
C
Brock
D J
DJ
Van Heyningen
V
V
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D000939:Epitopes / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D007781:Lactoferrin / Q000032:analysis* / Q000276:immunology; D007782:Lactoglobulins / Q000032:analysis*; D007951:Leukemia, Myeloid / Q000097:blood; D051379:Mice
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007782:Lactoglobulins; D007781:Lactoferrin