Expression of the cell proliferation-associated nuclear antigen reactive with the Ki-67 monoclonal antibody by cells of the skeletal system in humans and other species.
The cell proliferation-associated nuclear antigen recognized by the Ki-67 monoclonal antibody was detected immunohistochemically in cells from skeletal tissues of humans, sheep and rabbits. In each case the distribution of Ki-67-positive cells was identical and consistent with the known distribution of dividing cells in growing long bones. The antigen recognized by Ki-67 is detectable only after fixation in cold acetone. In the 5-day-old rabbit, labelling with tritiated thymidine [( 3H]TdR) in vivo, followed subsequently by a combination of Ki-67 immunohistochemistry and autoradiography for [3H]TdR, demonstrated that, in addition to the expression of Ki-67 by the majority of [3H]TdR labelled cells, other cells associated with proliferating populations also expressed the Ki-67 antigen. This suggests that the Ki-67 antibody identifies cycling cells in the skeleton in S-phase, as well as in other phases of the cell cycle. In Ki-67 reactive species, this method is a potentially useful tool for the study of cell proliferation in skeletal tissues in vivo and in vitro, having the advantage of not requiring prior labelling with DNA precursors.
Nuffield Department of Orthopaedic Surgery, University of Oxford, Headington, England.
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D000096:Acetone; D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis*; D001345:Autoradiography; D001846:Bone Development; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D001842:Bone and Bones / Q000166:cytology* / Q000196:embryology / Q000276:immunology; D002356:Cartilage / Q000276:immunology; D002455:Cell Division; D003080:Cold Temperature; D004247:DNA / Q000096:biosynthesis; D005404:Fixatives; D005865:Gestational Age; D006132:Growth Plate / Q000166:cytology / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen; D011817:Rabbits; D012756:Sheep
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D005404:Fixatives; D019394:Ki-67 Antigen; D000096:Acetone; D004247:DNA