[Dependence of ion transport across the plasma membrane on the density of the cell culture. I. Ion flows and the potassium and sodium content in 3 Chinese hamster cell lines (CHO)].
Cation transport has been investigated in three lines of Chinese ovary cells CHO-K1 during the cell culture growth. With the increase in the cell density potassium and sodium contents decreased from 1.2 to 0.8-0.5 and from 0.5 to 0.15-0.1 mmole/g protein, respectively. The time courses of potassium and sodium changes were different, and the increase in intracellular K/Na ratio from 1.5-2.0 to 5-10 with the increase in cell density was revealed. The rubidium influx was found to decrease during the culture growth mainly due to the decrease in ouabain-inhibitable and (ouabain + furosemide)- non-inhibitable influxes. The changes in cation fluxes and cation contents were observed in transformed cells without contact inhibition of division and were considered as a manifestation of density-dependent alterations of plasma membrane.
Marakhova
I I
II
Pospelova
T V
TV
Vinogradova
T A
TA
Vereninov
A A
AA
Ignatova
T N
TN
D000818:Animals; D001692:Biological Transport; D002452:Cell Count; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D007473:Ion Channels / Q000378:metabolism*; D010053:Ovary; D011188:Potassium / Q000032:analysis / Q000378:metabolism*; D012413:Rubidium / Q000378:metabolism; D012964:Sodium / Q000032:analysis / Q000378:metabolism*
D007473:Ion Channels; D012964:Sodium; D012413:Rubidium; D011188:Potassium
Zavisimost' transporta ionov cherez plazmaticheskuiu membranu ot plotnosti kletochnoĭ kul'tury. I. Ionnye potoki i soderzhanie kaliia i natriia u trekh linií kletok kitaĭskogo khomiachka CHO.