Immunohistochemical colocalization of glucagon, serotonin, and aromatic L-amino acid decarboxylase in islet A cells of chicken pancreas.
The identity of monoamine-emitted, formaldehyde-induced fluorescence in some pancreatic islet cells was studied in pancreatic tissue of male chickens by fluorescence and immunohistochemistry either on the same tissue section or on serial tissue sections. Pancreatic islet cells emitting intense formaldehyde-induced fluorescence also react immunohistochemically with antisera directed against glucagon, serotonin and aromatic L-amino acid decarboxylase. These results show that chicken pancreatic islet A cells contain glucagon, serotonin, and aromatic L-amino acid decarboxylase, an enzyme involved in the synthesis of serotonin. The islet B cells identified with anti-insulin immunoreactivity, which displayed a very weak formaldehyde-induced fluorescence, did not react with anti-serotonin serum.
Watanabe
T
T
Department of Veterinary Anatomy, Faculty of Agriculture, Nagoya University, Japan.
Morikami
T
T
Nagatsu
I
I
D000818:Animals; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000378:metabolism*; D002645:Chickens / Q000378:metabolism*; D005934:Glucagon / Q000378:metabolism*; D007150:Immunohistochemistry; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism*; D008297:Male; D012701:Serotonin / Q000378:metabolism*
D012701:Serotonin; D005934:Glucagon; D001142:Aromatic-L-Amino-Acid Decarboxylases