Flow cytometric enumeration of reticulocyte in the peripheral blood from canine infected with Babesia gibsoni.
Peripheral blood samples from dogs infected with Babesia gibsoni were analyzed by a flow cytometer for the percentage of reticulocytes after staining with a membrane-permeable fluorochrome, thiazole orange. Though thiazole orange has been reported to stain human reticulocytes and Plasmodium-infected erythrocytes, number of positive cells determined by the flow cytometry did not include that of erythrocytes infected with Babesia gibsoni. Analysis of 51 samples revealed a correlation coefficient of 0.96 as compared to the conventional determination by light microscopy. Separation of reticulocytes from Babesia gibsoni-infected erythrocytes by flow cytometry with or without the stain remained unresolved.
Department of Veterinary Science, College of Agriculture, University of Osaka Prefecture, Japan.
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Uemura
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Suzuki
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Ohnishi
D000818:Animals; D001404:Babesiosis / Q000097:blood*; D004283:Dog Diseases / Q000097:blood*; D004285:Dogs; D004906:Erythrocyte Count / Q000662:veterinary; D005434:Flow Cytometry; D012156:Reticulocytes