A transient outward current in isolated cells from the crista terminalis of rabbit heart.
Voltage-clamp experiments were carried out with the objective of identifying and characterizing the time- and voltage-dependent properties of a transient outward current recorded in single myocytes from the crista terminalis region of the rabbit heart. A collagenase enzymic dispersion procedure similar to that described by Desilets & Horackova (1982) was used to obtain these viable individual myocytes. Transmembrane ionic currents were recorded using a single micro-electrode voltage-clamp technique. In experiments aimed at studying a tetrodotoxin-resistant transient inward current, (ICa); a transient outward current was consistently recorded following blockade of ICa with Cd2+ (5 X 10(-4) M). The time and voltage dependence of the activation and inactivation of this current were measured. Its steady-state inactivation curve spans the voltage range -70 to -10 mV, and it is activated between -20 and +10 mV. The reversal potential of this transient outward current is approximately -75 mV in [K+]O 5 mM, suggesting that it is carried mainly by K+. This transient outward current can be inhibited completely by external application of 4-aminopyridine (4-AP, 3 mM). The time- and voltage-dependent properties, the reversal potential, and the sensitivity to 4-AP of this transient outward current are all very similar to those of a transient outward current first identified in molluscan neurones. Hence, we have labelled it, IA. Selective inhibition of IA and knowledge of its voltage- and time-dependent properties yield specific predictions concerning its role in the action potential of isolated crista terminalis cells. Consistent with these predictions, a decrease in stimulus rate is found to decrease the duration of the action potential and vice versa; and application of effective doses of 4-AP results in a substantial lengthening of the action potential. These results are discussed in terms of the possible physiological role of IA in subsidiary or follower pace-maker tissue, and the anatomical and physiological heterogeneity of the sino-atrial node region of the rabbit heart.
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Giles
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van Ginneken
D015761:4-Aminopyridine; D000200:Action Potentials / Q000187:drug effects; D000631:Aminopyridines / Q000494:pharmacology; D000818:Animals; D006321:Heart / Q000502:physiology*; D066298:In Vitro Techniques; D007473:Ion Channels / Q000502:physiology*; D007700:Kinetics; D009206:Myocardium / Q000166:cytology; D011188:Potassium / Q000502:physiology; D011817:Rabbits; D013779:Tetrodotoxin / Q000494:pharmacology
D000631:Aminopyridines; D007473:Ion Channels; D013779:Tetrodotoxin; D015761:4-Aminopyridine; D011188:Potassium