The effect of 12-O-tetradecanoylphorbol-13-acetate (TPA) on phospholipid metabolism of human epidermal keratinocytes in culture.
The tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) stimulated the release of [3H]choline from prelabelled membrane phospholipids of cultured keratinocytes obtained from normal human skin. In contrast, TPA in the concentration range of 10(-12) to 10(-6) g/ml failed to induce deacylation of [3H]arachidonic acid or stimulate [3H]prostaglandin production in prelabelled keratinocytes. In addition, TPA did not induce [3H]choline incorporation into the membrane phospholipids of these cells. The previously reported inability of TPA to stimulate a proliferative response in these cell cultures may be related to the resistance of these cells to TPA-induced alterations of arachidonate metabolism.
V A
VA
DeLeo
S
S
Scheide
H
H
Horlich
D
D
Hanson
W
W
Southern
L C
LC
Harber
I B
IB
Weinstein
D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D002794:Choline / Q000378:metabolism; D004789:Enzyme Activation; D000078404:Epidermal Cells; D004817:Epidermis / Q000187:drug effects / Q000378:metabolism*; D005312:Fetal Blood / Q000502:physiology; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D007633:Keratins / Q000378:metabolism; D010740:Phospholipases / Q000032:analysis; D010743:Phospholipids / Q000378:metabolism*; D011453:Prostaglandins / Q000096:biosynthesis; D012878:Skin Neoplasms / Q000139:chemically induced; D013755:Tetradecanoylphorbol Acetate; D014316:Tritium
D001095:Arachidonic Acids; D010743:Phospholipids; D011453:Prostaglandins; D014316:Tritium; D016718:Arachidonic Acid; D007633:Keratins; D010740:Phospholipases; D002794:Choline; D013755:Tetradecanoylphorbol Acetate; D006854:Hydrocortisone