A simple non-isotopic method for the quantitation of red cell-bound immunoglobulin.
A method was developed for the estimation of the number of molecules of immunoglobulin bound to red cells. Solubilization of sensitized, washed red cells was followed by an enzyme-linked immunosorbent assay of immunoglobulin concentration (SOL-ELISA). This assay gave results that were comparable to isotopic methods, was reproducible, utilised inexpensive reagents and was relatively quick and simple to perform.
UK Transplant Service, Bristol.
B M
BM
Kumpel
D000911:Antibodies, Monoclonal; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G