Isolation and biochemical characterization of the microsomal fraction from the digestive gland of mussel Mytilus galloprovincialis Lam.
A procedure to prepare microsomes from the mussel digestive gland is proposed. The data concerning the biochemical characterization of this subcellular fraction shows a typical RNA:protein ratio, but the presence of hydrolytic enzymes was also found; therefore a mixture of hydrolase inhibitors to study the different biochemical characteristics was used. The biochemical data demonstrate that glucose-6-phosphatase activity (G6Pase), a typical microsomal marker in mammalian cells, is not present in mussel digestive gland microsomes but a high non-specific phosphatase activity was detected. Benzo[a]pyrene hydroxylase activity was found to be present although in a minimal amount. The evaluation of the molecular weight of the rRNA demonstrates that the larger ribosomal subunit contains RNA of Mr 1.40 X 10(-6) (approximately 26S) and the smaller subunit is composed of RNA of Mr 0.65 X 10(-6) (18S). The data from mussel digestive gland microsomes was compared with that experimentally obtained from rat liver microsomes and discussed from a functional or an evolutionary point of view.
A
A
Viarengo
M
M
Pertica
G
G
Mancinelli
S
S
Palmero
M
M
Orunesu
D000818:Animals; D001491:Basophils / Q000378:metabolism; D001579:Benzopyrene Hydroxylase / Q000378:metabolism; D049872:Bivalvia / Q000378:metabolism*; D004064:Digestive System / Q000378:metabolism* / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D005088:Exocrine Glands / Q000378:metabolism* / Q000648:ultrastructure; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology; D008861:Microsomes / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D012313:RNA / Q000378:metabolism
D011506:Proteins; D012313:RNA; D001579:Benzopyrene Hydroxylase; D005952:Glucose-6-Phosphatase