New phase supports for liquid-liquid partition chromatography of biopolymers in aqueous poly(ethyleneglycol)-dextran systems. Synthesis and application for the fractionation of DNA restriction fragments.
New phase supports for liquid-liquid partition chromatography, using aqueous poly(ethyleneglycol)-dextran systems have been developed by grafting linear polyacrylamide chains on to premanufactured chromatographic supports carrying primary or secondary aliphatic hydroxyl functions on their surface. Columns prepared from such supports have a higher binding capacity for the dextran-rich stationary phase and much higher performances than columns prepared from cellulose, the previously used phase support for this system. Test separations of DNA restriction fragments, ranging from 11 base pairs to 3829 base pairs, document a high resolution for DNA fragments larger than 200 base pairs.
Müller
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D001482:Base Composition; D001704:Biopolymers; D002853:Chromatography, Liquid / Q000295:instrumentation*; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D003911:Dextrans; D004591:Electrophoresis, Polyacrylamide Gel; D008433:Mathematics; D011092:Polyethylene Glycols
D001704:Biopolymers; D003911:Dextrans; D011092:Polyethylene Glycols; D004247:DNA; D004262:DNA Restriction Enzymes