An improved method of antigen detection on nitrocellulose: in situ staining of alkaline phosphatase conjugated antibody.
A biological stain for alkaline phosphatase was applied to detect immune complexes immobilized on nitrocellulose. This method was sensitive and useful for in situ staining of antigens, in dot-immunobinding assays and immunoblots. In addition to its sensitivity, the staining of alkaline phosphatase conjugated antibody was also highly stable, and allowed a permanent record of original immunochemical data. A rapid and simple method is also described using this technique for screening translation products of recombinant clones.
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D000469:Alkaline Phosphatase / Q000032:analysis*; D000907:Antibodies, Bacterial / Q000032:analysis; D000936:Antigen-Antibody Complex / Q000032:analysis*; D001426:Bacterial Proteins / Q000276:immunology; D003101:Collodion; D005069:Evaluation Studies as Topic; D007124:Immunoenzyme Techniques; D011994:Recombinant Proteins / Q000276:immunology; D013194:Staining and Labeling / Q000379:methods*; D013295:Streptococcus mutans / Q000276:immunology
D000907:Antibodies, Bacterial; D000936:Antigen-Antibody Complex; D001426:Bacterial Proteins; D011994:Recombinant Proteins; D003101:Collodion; D000469:Alkaline Phosphatase