A cell surface marker of thymus-dependent lymphocytes in Xenopus laevis is identifiable by mouse monoclonal antibody.
A mouse monoclonal antibody (mAb), named XT-1 (IgG2b,lambda), was prepared against J strain Xenopus laevis thymocytes. The determinant recognized by this mAb (XT-1 determinant) was expressed by 92-98% thymocytes and 22-37% splenic lymphocytes from intact J strain frogs and larvae, as analyzed by flow cytofluorometry. The XT-1 determinant was also expressed by 20-30% peripheral blood lymphocytes and 5-11% bone marrow lymphocytes from adult, and 11-17% liver lymphocytes from larval J strain Xenopus. Early larval thymectomy depleted XT-1-positive cells in the spleen to less than 2% of the total lymphocyte population. Double immunofluorescence experiments revealed that XT-1+ lymphocytes in the spleen are surface IgM-. The strain distribution study on the expression of XT-1 determinant suggests the allelic polymorphism of the XT-1 determinant. The surface antigen identified by the present XT-1 mAb may provide an important marker for lymphocytes in thymus-dependent lineage in Xenopus.
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D000939:Epitopes; D051379:Mice / Q000276:immunology; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*; D013934:Thymectomy; D014018:Tissue Distribution; D014982:Xenopus laevis / Q000097:blood*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000939:Epitopes