The microchromatographic measurement of fetal hemoglobin levels in hemoglobin C-associated conditions.
We report the microchromatographic estimation of fetal hemoglobin (Hb F) in 3 conditions associated with hemoglobin C: hemoglobin C-hereditary persistence of fetal hemoglobin (Hb C-HPFH), homozygous hemoglobin C disease, and hemoglobin SC disease. In the case of Hb C-HPFH (Case 1) the Hb F level by alkali denaturation was 32.5% while the Hb F level by microchromatography was 35.3%. In both cases of homozygous Hb C disease and Hb SC disease the Hb F level by alkali denaturation was less than 1% and the microchromatographic Hb F level was 0.7%. We conclude that microchromatographic methods for determination of Hb F levels can be employed in the Hb C-HPFH, homozygous Hb C, and Hb SC conditions. To our knowledge, the microchromatographic determination of Hb F levels has not been applied previously to the Hb C-HPFH condition or Hb C disease.
J S
JS
Krauss
M H
MH
Jonah
C R
CR
Baisden
D Y
DY
Parnell
A E
AE
Neely
P
P
Mohorn
D000293:Adolescent; D000328:Adult; D002852:Chromatography, Ion Exchange / Q000379:methods; D004588:Electrophoresis, Cellulose Acetate / Q000379:methods; D005319:Fetal Hemoglobin / Q000032:analysis*; D006444:Hemoglobin C / Q000032:analysis*; D006801:Humans; D005779:Immunodiffusion; D008297:Male; D013053:Spectrophotometry
D006444:Hemoglobin C; D005319:Fetal Hemoglobin