Mechanism of interferon action--structural requirement of ligand and cellular specificity.
We had reported the existence of 2'-5'P3A3 receptor on the peritoneal macrophages from Wistar rat. In this paper data are given to demonstrate that the ligand, for binding with 2'-5'P3A3 receptor and enhancing the phagocytosis of macrophages, must have a three-phosphate radical on its 5'-terminus and at least three adenylate residues in 2'-5'oligoadenylate. (3H)-2'-5'P3A3 can also bind on macrophages from other animals; and the binding of (3H)-2'-5'P3A3 on Hepatoma cell line 7402 is lower than that on macrophages; whereas it is very low (binding ratio less than 1%) on erythrocytes (from rabbit, rat and mouse) or human embryo lung fibroblast cells. The binding conditions of the ligand with 2'-5'P3A3 receptor have also been investigated.
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D000227:Adenine Nucleotides / Q000494:pharmacology*; D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D002460:Cell Line; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D008024:Ligands; D008113:Liver Neoplasms; D008264:Macrophages / Q000276:immunology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009843:Oligoribonucleotides / Q000494:pharmacology*; D010587:Phagocytosis / Q000187:drug effects*; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D011955:Receptors, Drug / Q000276:immunology; D013045:Species Specificity
D000227:Adenine Nucleotides; D008024:Ligands; D009843:Oligoribonucleotides; D011955:Receptors, Drug; C016964:adenosine triphosphate adenosine monophosphate adenosine monophosphate; D007372:Interferons