Comparison of different markers on blood lymphocytes of chronic lymphocytic leukemia.
Mononuclear cells (MNC) of 17 patients suffering from B chronic lymphocytic leukemia (B-CLL) were analysed by various immunological methods. The B cell nature of CLL cell was determined by classical tests (MRBC-rosette-test, immunofluorescence test for detection of membrane bound immunoglobulins). The cytochemical detection of the new T-cell marker dipeptidyl peptidase IV (DP IV) was found to be suitable for the characterization of B-CLL. The B-CLL cells showed granular pattern of alpha-naphthylacetate esterase (ANAE) reaction and binding of the monoclonal pan T antibody BL-T2. These non typical reactions for normal B lymphocytes can be used for differential diagnosis of B-CLL in combination with other reliable T cell markers. Avoiding the separation of T cells, the mixed rosette assay was used to enumerate Fc-IgG-receptor bearing T(TG) and non T cells. Both cell populations were found to be significantly elevated in MNC of B-CLL.
Götze
D
D
Fiebig
H
H
Mansfeld
H W
HW
Mey
U
U
Schön
E
E
Uhle
R
R
Ansorge
S
S
D000368:Aged; D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D006801:Humans; D007945:Leukemia, Lymphoid / Q000276:immunology*; D008875:Middle Aged; D009283:Naphthol AS D Esterase / Q000378:metabolism; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000145:classification / Q000201:enzymology / Q000276:immunology
D000911:Antibodies, Monoclonal; D009283:Naphthol AS D Esterase