Action of spermidine, N1-acetylspermidine, and N8-acetylspermidine at apurinic sites in DNA.
The cleavage efficiency of spermidine and its acetyl derivatives (N1-acetylspermidine and N8-acetylspermidine) at apurinic sites in DNA were examined by PAGE-urea analysis. The three polyamines induced different rates of cleavage when compared at 1 mM concentrations. The order of effectiveness were: spermidine greater than N8-acetylspermidine greater than N1-acetylspermidine. Thus a decrease in efficiency was observed when the first order amino-groups of spermidine were blocked. The N-8amino-group of spermidine was less effective in inducing cleavage at AP-sites than the N1-amino-group. Among several proposed models of polyamine-DNA interactions, our results can best be explained by the model postulated by Liquori et al.
Haukanes
B I
BI
Laboratory of Biotechnology, University of Bergen, Norway.
Szajko
K
K
Helland
D E
DE
D001080:Apurinic Acid; D001483:Base Sequence; D004247:DNA; D006868:Hydrolysis; D008961:Models, Structural; D008969:Molecular Sequence Data; D010957:Plasmids; D011119:Polynucleotides; D013095:Spermidine / Q000031:analogs & derivatives*
D011119:Polynucleotides; D001080:Apurinic Acid; C030223:N(8)-acetylspermidine; C017988:N(1)-acetylspermidine; D004247:DNA; D013095:Spermidine