Modulation of Mn2+ accumulation in cultured rat neuronal and astroglial cells.
The effects of physiological concentrations of K+ on Mn2+ accumulation were compared in rat glial cells and neurons in culture. Increasing the K+ concentration in growth medium increased significantly the Mn2+ level of the cultivated cells, with glial cells more affected than neurons. Ethanol markedly increased the Mn2+ accumulation within glia but not within neurons while ouabain caused inhibition of Mn2+ uptake with neurons and glial cells. A modulation of the total protein synthesis by Mn2+ and ethanol level in the growth medium was observed with glial cells. These data suggest that the mechanisms involved in Mn2+ accumulation in glial cells are different from those present in neurons. Moreover, the results are consistent with the hypothesis that Mn2+ plays a regulatory role in glial cell metabolism.
Centre de Neurochimie du CNRS, Strasbourg, France.
G
G
Tholey
L
L
Megias-Megias
F C
FC
Wedler
M
M
Ledig
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D000431:Ethanol / Q000494:pharmacology; D008345:Manganese / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D051381:Rats
D009419:Nerve Tissue Proteins; D000431:Ethanol; D008345:Manganese; D010042:Ouabain; D011188:Potassium