An X-ray diffraction analysis of oriented lipid multilayers containing basic proteins.
X-ray diffraction techniques have been used to study the structures of lipid bilayers containing basic proteins. Highly ordered multilayer specimens have been formed by using the Langmuir-Blodgett method in which a solid support is passed through a lipid monolayer held at constant surface pressure at an air/water interface. If the lipid monolayer contains acidic lipids then basic proteins in the aqueous subphase are transferred with the monolayer and incorporated into the multi-membrane stack. X-ray diffraction patterns have been recorded from multilayers of cerebroside sulphate and 40% (molar) cholesterol both with and without polylysine, cytochrome c and the basic protein from central nervous system myelin. Electron density profiles across the membranes have been derived at between 6 A and 12 A resolution. All of the membrane profiles have been placed on an absolute scale of electron density by the isomorphous exchange of cholesterol with a brominated cholesterol analog. The distributions and conformations of the various basic proteins incorporated within the cerebroside sulphate/cholesterol bilayer are very different. Polylysine attaches to the surface of the lipid bilayer as a fully extended chain while cytochrome c maintains its native structure and attaches to the bilayer surface with its short axis approximately perpendicular to the membrane plane. The myelin basic protein associates intimately with the lipid headgroups in the form of an extended molecule, yet its dimension perpendicular to the plane of the membrane of approx. 15 A is consistent with the considerable degree of secondary structure found in solution. In the membrane plane, the myelin basic protein extends to cover an area of about 2500 A2. There is no significant penetration of the protein into the hydrocarbon region of the bilayer or, indeed, beyond the position of the sulphate group of the cerebroside sulphate molecule.
MacNaughtan
W
W
Snook
K A
KA
Caspi
E
E
Franks
N P
NP
D015502:Absorptiometry, Photon; D000818:Animals; D001923:Brain Chemistry; D002554:Cerebrosides / Q000032:analysis; D002784:Cholesterol / Q000032:analysis; D003574:Cytochrome c Group / Q000032:analysis; D008051:Lipid Bilayers / Q000032:analysis*; D008433:Mathematics; D008565:Membrane Proteins / Q000032:analysis*; D004676:Myelin Basic Protein / Q000032:analysis*; D011312:Pressure; D011817:Rabbits; D014961:X-Ray Diffraction
D002554:Cerebrosides; D003574:Cytochrome c Group; D008051:Lipid Bilayers; D008565:Membrane Proteins; D004676:Myelin Basic Protein; D002784:Cholesterol