Fluorescence study of renal cell carcinoma with antibodies to renal tubular antigens, intermediate filaments, and lectins.
A fluorescence study was performed in 16 renal cell carcinomas using antibodies to renal tubular antigens (RTA), two intermediate filaments, cytokeratin and vimentin, and two lectins, soybean agglutinin (SBA) and peanut agglutinin (PNA). We observed the presence of RTA, cytokeratin, and vimentin in all of our specimens. The expression of vimentin, the cytoskeletal protein of mesenchymal cells, was considered to be very interesting feature of the tumor. Binding sites of SBA, normally present in glomeruli, proximal and distal tubules, were detectable in the neoplastic cells in only 37.5% of our specimens. PNA did not react with the tumor except for the small area of 2 specimens. Lectins may be useful for estimating the characteristics or renal cell carcinoma including its malignant potentials, and antibodies to RTA and intermediate filaments seem to be available for the diagnosis of the tumor in metastatic lesions.
Iizumi
T
T
Yazaki
T
T
Kanoh
S
S
Koiso
K
K
Koyama
A
A
Tojo
S
S
D000906:Antibodies / Q000276:immunology*; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000276:immunology; D002292:Carcinoma, Renal Cell / Q000276:immunology*; D003599:Cytoskeleton / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007382:Intermediate Filaments / Q000276:immunology*; D007633:Keratins / Q000276:immunology; D007680:Kidney Neoplasms / Q000276:immunology*; D007684:Kidney Tubules / Q000276:immunology*; D037102:Lectins / Q000276:immunology*; D019887:Peanut Agglutinin; D037121:Plant Lectins; D030262:Soybean Proteins; D014746:Vimentin / Q000276:immunology; D010835:Phytohemagglutinins
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000941:Antigens; D000951:Antigens, Neoplasm; D007633:Keratins; D037102:Lectins; D019887:Peanut Agglutinin; D037121:Plant Lectins; D030262:Soybean Proteins; D014746:Vimentin; C035812:renal tubular antigen; C028824:soybean lectin; D010835:Phytohemagglutinins