The effect of IgA immune complexes on the proliferation of cultured human mesangial cells.
To investigate the role of IgA-containing circulating immune complexes (IgA-CIC) in mesangial cell proliferation, human mesangial cells were cultured with sera or IgA isolates from IgA-CIC-positive IgA nephropathy patients and negative controls. A solid phase anti-C3d enzyme-linked immunosorbent assay (ELISA) was used for the detection of IgA-CIC. Sera stimulated the proliferation of the mesangial cells at both 24 and 72 hours' incubation. However, there was no difference in the proliferation of cells incubated with sera from IgA-CIC-positive patients and negative controls. IgA-CIC-positive and negative IgA isolates both stimulated proliferation of mesangial cells at 24 hours' incubation. Our data do not support the hypothesis that IgA-CIC are directly responsible for mesangial cell proliferation.
Fujii
K
K
Renal Unit, Royal Adelaide Hospital, South Australia.
Muller
K D
KD
Clarkson
A R
AR
Woodroffe
A J
AJ
D000936:Antigen-Antibody Complex / Q000502:physiology*; D002455:Cell Division / Q000276:immunology; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005920:Glomerular Mesangium / Q000166:cytology*; D005922:Glomerulonephritis, IGA / Q000276:immunology / Q000473:pathology*; D006801:Humans; D007070:Immunoglobulin A / Q000502:physiology*
D000936:Antigen-Antibody Complex; D007070:Immunoglobulin A