Substance P and 5-hydroxytryptamine immunoreactive presynaptic boutons on presumed alpha-motoneurons in the chicken ventral horn.
Substance P and 5-hydroxytryptamine containing presynaptic boutons on presumed alpha-motoneurons were studied in the chicken ventral horn using the indirect antibody peroxidase-antiperoxidase technique in conjunction with monoclonal antibodies to 5-hydroxytryptamine and substance P both at light and electron microscopic levels. At the light microscopic level, substance P immunoreactivity was observed as large dot-like structures in close apposition to the soma and dendrites of presumed alpha-motoneurons. Individual immunoreactive structures were separated from each other. On the other hand, 5-hydroxytryptamine immunoreactivity was observed as many small dot- or fiber-like structures outlining the soma and dendrites of large neurons in the ventral horn. Electron microscopically, 5-hydroxytryptamine immunoreactive presynaptic boutons were identified as the type which contained elongated dense cored vesicles concomitant with flattened clear vesicles, while the substance P immunoreactivity was observed in presynaptic boutons which contained spherical dense cored vesicles (diameter range 60-110 nm) together with spherical clear vesicles. Both types of dense cored vesicles were often adjacent to the presynaptic membrane of synaptic specialization. These immunocytochemical results suggest that alpha-motoneurons may be directly modulated by substance P and 5-hydroxytryptamine in the chicken.
Atsumi
S
S
Sakamoto
H
H
Yokota
S
S
Fujiwara
T
T
D000818:Animals; D002645:Chickens; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D009046:Motor Neurons / Q000032:analysis* / Q000648:ultrastructure; D012701:Serotonin / Q000032:analysis*; D013116:Spinal Cord / Q000032:analysis* / Q000648:ultrastructure; D013373:Substance P / Q000032:analysis*; D013569:Synapses / Q000032:analysis*
D012701:Serotonin; D013373:Substance P