Sheep lymphocyte antigens (OLA). I. Major histocompatibility complex class I molecules.
Three monoclonal antibodies, SBU.I 41-17, 41-19 and 41-28, have been produced which recognize sheep Class I major histocompatibility complex antigens (OLA). All three antibodies are able to precipitate a heavy chain of 44,000 MW and a smaller beta 2-microglobulin of 12,000 MW from 125I-surface labelled lymphocytes. The antibodies have been used to localize OLA Class I antigens in lymphoid and non-lymphoid tissues using indirect immunoperoxidase histological staining and cytofluorograph analyses. Evidence suggests that the three antibodies are directed against monomorphic determinants but that they recognize different epitopes.
Gogolin-Ewens
K J
KJ
Mackay
C R
CR
Mercer
W R
WR
Brandon
M R
MR
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000032:analysis; D006649:Histocompatibility Antigens / Q000032:analysis*; D007124:Immunoenzyme Techniques; D008214:Lymphocytes / Q000276:immunology*; D008221:Lymphoid Tissue / Q000276:immunology; D008285:Major Histocompatibility Complex; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D012756:Sheep / Q000276:immunology*; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006649:Histocompatibility Antigens