Determination of mutagenic activities in different fractions of automobile exhaust condensate by the Salmonella/oxygenase mutagenicity test system.
Automobile exhaust condensate of a passenger car (gasoline engine) was separated into fractions of 2-3 rings containing -, 4-7 rings containing polycyclic aromatic hydrocarbons (PAHs) and PAH-free fractions. All fractions were tested for mutagenicity by the Ames system. The highest dose-dependent increase in revertant colonies was found for the 4-7 ring PAH-fraction when tested with Salmonella typhimurium TA 98 and TA 100. These results are compatible with data obtained in in-vivo tests by previous investigations. The mutagenicity of these fractions in the absence of the oxygenase was negligible.
K
K
Norpoth
J
J
Jacob
G
G
Grimmer
E
E
Mohtashamipur
D000818:Animals; D001564:Benzo(a)pyrene / Q000009:adverse effects; D002849:Chromatography, Gas; D008297:Male; D051379:Mice; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens; D009154:Mutation; D010105:Oxygenases / Q000378:metabolism; D011083:Polycyclic Compounds / Q000009:adverse effects*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D001335:Vehicle Emissions / Q000633:toxicity*
D009153:Mutagens; D011083:Polycyclic Compounds; D001335:Vehicle Emissions; D001564:Benzo(a)pyrene; D010105:Oxygenases