Characterisation of the European seal morbillivirus.
The ELISA test originally developed for the detection of serum antibodies to rinderpest virus has been shown to detect cross-reacting antibodies in sera of diseased common and grey seals. Analysis of sera collected from various seal populations is in progress to establish the correlation between different morbillivirus neutralisation tests, ELISA tests and the disease status of the animals. RNA purified from post-mortem tissues removed from diseased seals has been analysed by hybridisation with cloned cDNAs made to various genes of canine distemper, peste des petits ruminants, rinderpest and measles viruses. This study has confirmed the presence of RNA sequences characteristic of a morbillivirus, but shown that the virus is not identical with any known morbillivirus. Work is in progress to determine the nucleotide sequences of clones carrying inserts homologous to morbillivirus genes isolated from cDNA made on a template of infected seal tissue RNA.
A.F.R.C. Institute for Animal Health, Pirbright Laboratory, Nr. Woking, Surrey, Great Britain.
C J
CJ
Bostock
T
T
Barrett
J R
JR
Crowther
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis* / Q000276:immunology; D010872:Caniformia; D003429:Cross Reactions; D015342:DNA Probes; D004797:Enzyme-Linked Immunosorbent Assay; D005060:Europe; D007106:Immune Sera / Q000276:immunology; D009693:Nucleic Acid Hybridization; D010252:Paramyxoviridae / Q000145:classification* / Q000235:genetics / Q000276:immunology; D011233:Precipitin Tests; D012367:RNA, Viral / Q000032:analysis*; D010253:Respirovirus Infections / Q000382:microbiology / Q000662:veterinary*; D046048:Seals, Earless; D012689:Sequence Homology, Nucleic Acid
D000914:Antibodies, Viral; D015342:DNA Probes; D007106:Immune Sera; D012367:RNA, Viral