Properties of BGP1, a poly(dG)-binding protein from chicken erythrocytes.
The chicken beta A-globin gene contains in the neighborhood of its 5' promoter a (dG)-homopolymer sequence 16 base pairs long. The 66 kD protein BGP1 (beta globin protein 1), isolated from chicken erythrocytes, has been shown to bind specifically to this sequence. We describe further purification of BGP1, measure its affinity for the beta A-globin promoter binding site, and analyze its binding properties. The minimal binding sequence is seven dG residues; methylation interference studies show that each of these residues contacts BGP1. Binding competition experiments employing (dG).(dC) oligomers of varying lengths also consistent with (dG)7 as a minimum recognition sequence. All of the data can be explained by a model in which BGP1 binds to any contiguous set of seven (dG) residues, so that the effective constant for binding to (dG)n is proportional to n minus 6. This behavior may be typical of proteins that bind specifically to repeated sequences.
Laboratory of Molecular Biology, National Institute of Diabetes, and Digestive and Kidney Diseases, Methesda, MD 20892.
S P
SP
Clark
C D
CD
Lewis
G
G
Felsenfeld
D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000378:metabolism*; D005914:Globins / Q000302:isolation & purification / Q000378:metabolism*; D008745:Methylation; D008969:Molecular Sequence Data; D011068:Poly G / Q000235:genetics / Q000378:metabolism*; D011131:Polyribonucleotides / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid
D011131:Polyribonucleotides; D011068:Poly G; C026628:poly(dG); D005914:Globins