Immunoreactive substance P release from skin nerves in the rat by noxious thermal stimulation.
We have examined by radioimmunoassay the substance P (IR-SP) content of superfusate obtained from skin blisters in the anesthetized rat. The blisters were produced on the footpad using a vacuum pump, and the blister base was superfused with tyrode solution. Continuous release of IR-SP was approximately 100 pg/h from the innervated footpad but was considerably reduced when the footpad had been surgically denervated 10-14 days previously. The IR-SP content was markedly increased in the innervated footpad following a local noxious heat stimulus of 50 degrees C applied for 30 s at 5-min intervals for 1 h. The results support the proposal that SP is a mediator of neurogenic inflammation induced by noxious skin heating.
Helme
R D
RD
Koschorke
G M
GM
Zimmermann
M
M
D000818:Animals; D001768:Blister / Q000378:metabolism; D001798:Blood Proteins / Q000032:analysis; D006358:Hot Temperature; D008297:Male; D009420:Nervous System / Q000378:metabolism; D009619:Nociceptors / Q000502:physiology; D010812:Physical Stimulation; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000294:innervation*; D013373:Substance P / Q000378:metabolism*
D001798:Blood Proteins; D013373:Substance P