Comparison of multiple HLA-A alleles at the DNA level by using Southern blotting and HLA-A-specific probes.
Numerous alleles of the HLA-A gene have been serologically identified. In this report we present a rapid and straightforward means to assess HLA-A polymorphism at the genomic level. Using 5' and 3' HLA-A-specific DNA probes and Southern blotting, we have placed the recognition sequences for five endonucleases relative to the coding regions of 15 HLA-A alleles. These data permit two interesting conclusions: four of the HLA-A alleles studied are associated with unique restriction fragments, and HLA-A alleles of a cross-reactive group are more closely related at the DNA level than are noncross-reactive alleles.
B H
BH
Koller
F E
FE
Ward
R
R
DeMars
H T
HT
Orr
D000483:Alleles; D003429:Cross Reactions; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D006680:HLA Antigens / Q000235:genetics* / Q000276:immunology; D015234:HLA-A Antigens; D006801:Humans; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis
D000939:Epitopes; D006680:HLA Antigens; D015234:HLA-A Antigens; D004247:DNA; D004262:DNA Restriction Enzymes