Differential production of interleukin 1 on the surface of biomaterials.
The production of cytokines on the surface of surgical biomaterials plays a major role in their biocompatibility. Membrane-associated interleukin 1 (mIL-1) is a cytokine found on the surface of macrophages activated by biomaterials. To better understand the host-foreign body interaction, we quantitated the production of mIL-1 on the surface of two materials commonly used in surgery, expanded polytef (ePTFE) and silicon elastomer (SE). The mean (+/- SD) level of mIL-1 produced by adherent cells to ePTFE significantly decreased from day 2 (13,746 +/- 3630 cpm per disk) compared with day 7 (2828 +/- 1304 cpm per disk). However, the level of mIL-1 produced by ePTFE-adherent cells was still markedly greater than the level of mIL-1 produced by cells adherent to SE (1877 +/- 1028 vs 1595 +/- 822 cpm per disk). These results indicate that ePTFE and SE elicit a differential host response in terms of cytokine production. This study may enhance our understanding of the cellular events on the surface of biomaterials that underlie clinical observations.
Krause
T J
TJ
Department of Surgery, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, New Brunswick 08903.
Robertson
F M
FM
Liesch
J B
JB
Wasserman
A J
AJ
Greco
R S
RS
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D007375:Interleukin-1 / Q000096:biosynthesis*; D007962:Leukocytes / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D011138:Polytetrafluoroethylene / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012826:Silicone Elastomers / Q000494:pharmacology*
D001672:Biocompatible Materials; D007375:Interleukin-1; D012826:Silicone Elastomers; D011138:Polytetrafluoroethylene