Single step enrichment of human peripheral blood basophils by Ficoll-Paque centrifugation.
A simple, rapid and economical method for the enrichment of basophils from peripheral blood with high recovery is described. Peripheral blood was layered over a Ficoll-Paque cushion and centrifuged. The Ficoll-Paque layer was aspirated taking care not to disturb the interphase and the buffy coat. The Ficoll-Paque layer contained most of the basophils. A recovery of 82% (65-95%) and a 23.5-fold enrichment of basophils with their morphology and biological activity preserved was achieved.
A A
AA
Miroli
B M
BM
James
M
M
Spitz
D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D001491:Basophils / Q000276:immunology; D002469:Cell Separation / Q000379:methods; D002498:Centrifugation / Q000379:methods; D005362:Ficoll; D006636:Histamine Release; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D007958:Leukocyte Count
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D005362:Ficoll; D007073:Immunoglobulin E