UvrABC nuclease complex repairs thymine glycol, an oxidative DNA base damage.
The UvrABC nuclease complex recognizes a wide spectrum of DNA lesions including pyrimidine dimers, bulky chemical adducts and O6-methylguanine. In this study we have demonstrated that the UvrABC complex is also able to incise PM2 DNA containing the oxidative DNA lesion, thymine glycol. However, DNA containing dihydrothymine, a lesion with a similar structure to thymine glycol, was not incised. The UvrABC complex was also able to incise DNA containing reduced apurinic sites or apurinic sites modified with O-alkyl hydroxylamines, but not DNA containing apurinic sites or urea residues. In vivo, in the absence of base-excision repair, nucleotide excision repair was operable on phi X-174 RF transfecting DNA containing thymine glycols. The level of the repair was found to be directly related to the level of the UvrABC complex. Thus, UvrABC-mediated nucleotide excision repair appears to play a role in the repair of thymine glycol, an oxidative DNA-base lesion that is produced by ionizing radiation or formed during oxidative respiration.
Department of Microbiology and Molecular Genetics, University of Vermont, Burlington 05405.
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Kow
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Wallace
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Van Houten
D001080:Apurinic Acid / Q000378:metabolism; D001435:Bacteriophages / Q000235:genetics / Q000378:metabolism; D004260:DNA Repair; D004279:DNA, Viral / Q000378:metabolism; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D005470:Fluorometry; D006088:Gram-Negative Aerobic Bacteria; D006898:Hydroxylamines / Q000378:metabolism; D010084:Oxidation-Reduction; D013379:Substrate Specificity; D013941:Thymine / Q000031:analogs & derivatives* / Q000378:metabolism; D014161:Transduction, Genetic; D014162:Transfection
D004279:DNA, Viral; D029968:Escherichia coli Proteins; D006898:Hydroxylamines; D001080:Apurinic Acid; C029389:thymine glycol; D004706:Endodeoxyribonucleases; C036385:endodeoxyribonuclease uvrABC; D013941:Thymine