Polymorphism and mapping of the IGF1 gene, and absence of association with stature among African Pygmies.
Probes detecting restriction fragment length polymorphisms (RFLPs) in the insulinlike growth factor (IGF1) gene were isolated and allele frequencies in different human populations determined. No difference was detected between the distribution of IGF1 alleles in Pygmies versus non-Pygmy black Africans, despite the proposal that a defect in the IGF1 gene might be responsible for Pygmy short stature. This was supported by the absence of a correlation of IGF1 genotype with height in the C.A.R. Pygmies. Polymerase chain reaction (PCR) and direct sequencing failed to demonstrate an alteration in the region upstream the IGF1 start site in Pygmies. Linkage analysis demonstrated that IGF1 is tightly linked to the phenylalanine hydroxylase gene on chromosome 12q22-24.1.
Department of Genetics, Stanford University, CA 94305.
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Bowcock
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Sartorelli
D000349:Africa; D000483:Alleles; D001483:Base Sequence; D001827:Body Height / Q000208:ethnology / Q000235:genetics*; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D005787:Gene Frequency; D015723:Gene Library; D008040:Genetic Linkage; D005838:Genotype; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D013002:Somatomedins / Q000235:genetics*
D013002:Somatomedins; D007334:Insulin-Like Growth Factor I