Scyliorhinin I and II: two novel tachykinins from dogfish gut.
Two peptides with tachykinin-like ability to contract longitudinal muscle from the guinea pig ileum were isolated from the intestine of the common dogfish, Scyliorhinus caniculus. The amino acid sequence of scyliorhinin I was established as Ala-Lys-Phe-Asp-Lys-Phe-Tyr-Gly-Leu-Met-NH2 and this peptide cross-reacted with antisera directed against the C-terminal region fo substance P. The amino acid sequence of scyliorhinin II was established as Ser-Pro-Ser-Asn-Ser-Lys-Cys-Pro-Asp-Gly-Pro-Asp-Cys-Phe-Val-Gly-Leu-Met- NH2 and this peptide cross-reacted with antisera directed against the C-terminal region of neurokinin A. The mammalian peptides substance P and neurokinin A were absent from the dogfish intestinal tissue.
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JM
Conlon
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Deacon
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O'Toole
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Thim
D000595:Amino Acid Sequence; D000818:Animals; D000936:Antigen-Antibody Complex; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D004284:Dogfish; D007106:Immune Sera; D007422:Intestines / Q000032:analysis*; D009842:Oligopeptides / Q000302:isolation & purification*; D010455:Peptides / Q000302:isolation & purification*; D011863:Radioimmunoassay; D013045:Species Specificity; D013329:Structure-Activity Relationship; D013373:Substance P / Q000032:analysis; D015320:Tachykinins
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D009842:Oligopeptides; D010455:Peptides; D015320:Tachykinins; C048918:scyliorhinin I; C048919:scyliorhinin II; D013373:Substance P