Morphological and biochemical changes of the pancreas in rats treated with acetaldehyde.
Daily intraperitoneal injections of acetaldehyde for 10 days to adult rats produced distinct morphological and biochemical changes in the exocrine pancreas, without affecting the body weight, pancreatic weight, and DNA, RNA, and protein content of the pancreas. By electronmicroscopy, although the number and size of the acinar zymogen granules appeared to be the same between the saline- and acetaldehyde-treated rats, the zymogen granules of the latter group showed decreased osmiophilia. Acinar mitochondria of the acetaldehyde-treated rats were found to be slightly swollen with dense granules, and plasma membrane fragments were often seen in the acinar lumen. Administration of acetaldehyde significantly decreased immunoreactive cationic trypsin (ogen) and total amylase activity in the pancreas, but not in the serum, where amylase activity was markedly increased. Basal secretion of amylase, trypsinogen, and chymotrypsinogen from isolated dispersed pancreatic acini of acetaldehyde-treated rats was increased by 40-50%. Nicotine (5-25 mM) induced a profound increase in secretion of the same enzymes from isolated acini of both saline- and acetaldehyde-treated rats, but in the latter group there was a concomitant rise in LDH release. Furthermore, CCK-8 (1 nM), secretin (1 microM), and carbachol (10 microM) either alone or in combination with nicotine (12.5 mM) produced a profound stimulation in amylase, trypsinogen, and chymotrypsinogen secretion from acini of both groups of rats. On the other hand, secretion of 3H-pulse-labeled proteins from isolated acini of acetaldehyde-treated rats by nicotine was decreased by approximately 50% compared with the controls.(ABSTRACT TRUNCATED AT 250 WORDS)
A P
AP
Majumdar
G D
GD
Vesenka
M A
MA
Dubick
G S
GS
Yu
J M
JM
DeMorrow
M C
MC
Geokas
D000079:Acetaldehyde / Q000494:pharmacology*; D000681:Amylases / Q000378:metabolism; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects; D002919:Chymotrypsinogen / Q000378:metabolism; D003594:Cytoplasmic Granules / Q000187:drug effects; D005260:Female; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008854:Microscopy, Electron; D008928:Mitochondria / Q000187:drug effects; D009538:Nicotine / Q000494:pharmacology; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D012633:Secretin / Q000494:pharmacology; D012844:Sincalide / Q000494:pharmacology; D014362:Trypsinogen / Q000378:metabolism
D012633:Secretin; D009538:Nicotine; D002217:Carbachol; D014362:Trypsinogen; D002919:Chymotrypsinogen; D007770:L-Lactate Dehydrogenase; D000681:Amylases; D000079:Acetaldehyde; D012844:Sincalide