Purification of two transcription factors required for initiation by mammalian RNA polymerase II.
We have purified two general transcription factors (FA and FE) necessary for specific transcription by mammalian RNA polymerase II to near homogeneity. Both activities are associated with peptides of approximately 33 kDa. FA and FE do not replace one another and show different kinetics of action in a sarkosyl block assay. In particular, FE participated in a rapid reaction after the formation of an initial complex with the other transcription factors. Furthermore, FE can associate with purified calf thymus RNA polymerase II.
Department of Human Genetics, Medical Research Institute, Tokyo Medical and Dental University, Japan.
T
T
Kawaguchi
S
S
Kitajima
Y
Y
Niho
T
T
Oda
J
J
Germino
S M
SM
Weissman
Y
Y
Yasukochi
D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000378:metabolism; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D008970:Molecular Weight; D010957:Plasmids; D012319:RNA Polymerase II / Q000378:metabolism*; D013698:Templates, Genetic; D013950:Thymus Gland / Q000201:enzymology; D014157:Transcription Factors / Q000302:isolation & purification* / Q000378:metabolism; D014158:Transcription, Genetic
D014157:Transcription Factors; D012319:RNA Polymerase II