Ubiquity of the repetitive epitope of the CS protein in different isolates of human malaria parasites.
Sporozoites of the human malaria Plasmodium falciparum and Plasmodium vivax obtained from a large number of endemic areas were screened with species-specific monoclonal antibodies that recognize the repeated epitopes of the respective circumsporozoite (CS) proteins. By using a two-site immunoradiometric assay, it was determined that all the parasite isolates of a given species react with a single monoclonal antibody, indicating the presence of a common repeated epitope. Polyacrylamide gel electrophoresis, followed by Western blot, showed that the CS proteins of the various isolates differed in their apparent m.w.
Zavala
F
F
Masuda
A
A
Graves
P M
PM
Nussenzweig
V
V
Nussenzweig
R S
RS
D000818:Animals; D000852:Anopheles; D000953:Antigens, Protozoan / Q000032:analysis* / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis* / Q000276:immunology; D000939:Epitopes / Q000032:analysis* / Q000276:immunology; D006801:Humans; D008288:Malaria / Q000276:immunology; D010963:Plasmodium falciparum / Q000254:growth & development / Q000276:immunology*; D010966:Plasmodium vivax / Q000254:growth & development / Q000276:immunology*; D015800:Protozoan Proteins; D012469:Salivary Glands / Q000469:parasitology; D013045:Species Specificity
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D000939:Epitopes; D015800:Protozoan Proteins; C041595:circumsporozoite protein, Protozoan