Hyperthermia and human leukocyte functions: DNA, RNA, and total protein synthesis after exposure to less than 41 degrees or greater than 42.5 degrees hyperthermia.
Hyperthermia is being used clinically in the treatment of neoplasms, and mononuclear leukocytes are commonly present in a heated tumor or in surrounding tissues. Therefore, we examined the effects of exposure of human mononuclear leukocytes to less than 41 degrees or greater than 42.5 degrees hyperthermia for 2 h. The ability of the cells to exclude dyes, used as a measurement of leukocyte viability, was not altered by such exposures. Exposure of the cells to less than 41 degrees hyperthermia generally did not alter unstimulated or mitogen-stimulated DNA, RNA, or total protein synthesis. In contrast, exposure to greater than 42.5 degrees hyperthermia resulted in decreased unstimulated RNA and total protein synthesis, as well as delayed synthesis of DNA, RNA, and total protein in response to stimulation with the optimal concentration of mitogen and decreased synthesis in response to suboptimal concentrations of mitogen.
Roberts
N J
NJ
Lu
S T
ST
Michaelson
S M
SM
D000328:Adult; D002470:Cell Survival; D004247:DNA / Q000096:biosynthesis*; D005260:Female; D006358:Hot Temperature; D006801:Humans; D066298:In Vitro Techniques; D007962:Leukocytes / Q000378:metabolism*; D008213:Lymphocyte Activation; D008297:Male; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*
D012313:RNA; D004247:DNA