Fluorographic detection of [3H]thymidine-labeled deoxyribonucleic acid using agarose gels infused with 2,5-diphenyloxazole prior to electrophoresis.
A fluorographic procedure for the detection of [3H]thymidine-labeled deoxyribonucleic acids electrophoresed in agarose gels was developed. 2,5-Diphenyloxazole (PPO) was added to the agarose solution before pouring of the gel for electrophoresis. This procedure did not interfere with the electrophoretic mobility of the DNA molecules. The radioactive detection efficiency was found to be improved over an existing procedure whereby the agarose gel was infused with PPO after electrophoresis with the aid of acetic acid.
J W
JW
Ludlow
K C
KC
Yuen
R A
RA
Consigli
D004247:DNA / Q000032:analysis*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis; D019003:Deoxyribonuclease HpaII; D004587:Electrophoresis, Agar Gel / Q000379:methods; D010080:Oxazoles; D010779:Photofluorography; D011120:Polyomavirus / Q000235:genetics; D013936:Thymidine
D004279:DNA, Viral; D010080:Oxazoles; D004247:DNA; C009012:2,5-diphenyloxazole; D004262:DNA Restriction Enzymes; D019003:Deoxyribonuclease HpaII; D013936:Thymidine