Repeat unit sequence variation in minisatellites: a novel source of DNA polymorphism for studying variation and mutation by single molecule analysis.
Variation in internal minisatellite structure can be analyzed by mapping variant repeat units within amplified alleles. A system capable of distinguishing greater than 10(70) allelic states at the human hypervariable locus D1S8 has been developed. Population surveys of internal allelic structure indicate that D1S8 alleles evolve rapidly along haploid chromosome lineages. Internal mapping of deletion mutant alleles physically selected from genomic DNA provides further evidence that germline and somatic mutations altering the number of allelic repeat units seldom if ever arise by unequal exchange between alleles. The existence of low level germline mosaicism for new mutants further indicates that many germline mutation events are premeiotic. Physical selection of new mutants also allows minisatellite mutation rates to be estimated directly in human DNA.
Department of Genetics, University of Leicester, England.
A J
AJ
Jeffreys
R
R
Neumann
V
V
Wilson
D000483:Alleles; D001483:Base Sequence; D002872:Chromosome Deletion; D004276:DNA, Satellite / Q000097:blood / Q000235:genetics* / Q000302:isolation & purification; D005821:Genetic Techniques; D014644:Genetic Variation; D006239:Haplotypes; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D015345:Oligonucleotide Probes; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D013094:Spermatozoa
D004276:DNA, Satellite; D015345:Oligonucleotide Probes