Regional and subcellular distribution of Thy-1 in human brain assayed by a solid-phase radioimmunoassay.
A solid-phase radioimmunoassay, specific for the monomeric form of human Thy-1, was developed and used for quantitation of the Thy-1 antigen in human brain tissue. Determination of Thy-1 in homogenates of 12 anatomically defined brain regions showed that Thy-1 is present throughout the human brain. However, significant variation was found in the expression of the glycoprotein in different regions. Thy-1 appears to be generally enriched within gray matter: caudate nucleus, cerebral cortex, and putamen were found to contain the highest Thy-1 concentration (approximately 2.5 micrograms Thy-1/mg protein). Interestingly, the cerebellar cortex contained only 25% of the Thy-1 concentration of cerebral gray matter. Cerebral subcortical white matter contained half the amount of Thy-1 compared to cerebral cortex. Determination of Thy-1 in subcellular fractions prepared from human brain biopsy tissue indicated that the highest relative concentration of Thy-1 is associated with synaptosomal membranes and myelin/axonal membrane fractions.
P
P
Almqvist
S R
SR
Carlsson
J A
JA
Hardy
B
B
Winblad
D000328:Adult; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000032:analysis* / Q000276:immunology; D001369:Axons / Q000276:immunology; D001921:Brain / Q000276:immunology*; D002421:Caudate Nucleus / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology; D002540:Cerebral Cortex / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D009186:Myelin Sheath / Q000276:immunology; D011699:Putamen / Q000276:immunology; D011863:Radioimmunoassay; D013574:Synaptosomes / Q000276:immunology; D018800:Thy-1 Antigens; D014018:Tissue Distribution
D000954:Antigens, Surface; D000939:Epitopes; D007106:Immune Sera; D018800:Thy-1 Antigens