Interleukin 2 receptor beta chain expressed in an oligodendroglioma line binds interleukin 2 and delivers growth signal.
Interleukin 2 (IL-2) is a potent growth factor for T lymphocytes, playing a crucial role in the immune response. In view of the considerable evidence that the immunoregulatory cytokines (or lymphokines) also play a role in the growth and differentiation of cells in the central nervous system (CNS), we examined the operation of the IL-2 system in a cell line of CNS origin by expressing a cDNA encoding the beta chain of the human IL-2 receptor (IL-2R beta, a 75-kDa protein). When the cDNA was expressed in a human oligodendroglioma cell line, ONS-21, the IL-2R beta bound IL-2 with an affinity similar to that in lymphoid cells (Kd, approximately 2 nM). Furthermore, cell proliferation ([3H]thymidine incorporation) was stimulated by IL-2. These results demonstrate that the same cytokine receptor is functional in cells of the immune system and CNS and point to a molecular mechanism that is similar for growth-signal transduction between lymphoid and neural cells but that may be different in other cells, such as fibroblasts.
Institute for Molecular and Cellular Biology, Osaka University, Japan.
Y
Y
Okamoto
S
S
Minamoto
K
K
Shimizu
H
H
Mogami
T
T
Taniguchi
D000906:Antibodies; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002999:Clone Cells; D004261:DNA Replication / Q000187:drug effects; D006801:Humans; D007376:Interleukin-2 / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology; D007700:Kinetics; D046911:Macromolecular Substances; D009837:Oligodendroglioma; D015375:Receptors, Interleukin-2 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014162:Transfection
D000906:Antibodies; D007376:Interleukin-2; D046911:Macromolecular Substances; D015375:Receptors, Interleukin-2