Characterization of a functional domain of human calpastatin.
Expression plasmids were constructed from the cDNA of human calpastatin to examine the contribution to the inhibition of calpain of highly conserved sequences in each of four repetitive domains. A series of deletion derivatives of domain 1 proteins, truncated at either the amino or carboxy terminus, were produced in E. coli. Deletion from the amino terminus past the amino terminal conserved sequence decreased the inhibition. When the middle conserved sequence, the M-sequence, was further deleted, no inhibition was detected, but deletion from the carboxy terminus past the carboxy terminal conserved sequence did not decrease the inhibition until the M-sequence was reached. Nuclear magnetic resonance and circular dichroism spectra showed that domain 1 has an unfolded structure. Peptides that contained the M-sequence and some neighboring sequences were synthesized to measure the minimum size of the inhibitory peptide, which was the M-sequence with the next six residues on the amino terminal side.
Biotechnology Research Laboratories, Takara Shuzo Co, Ltd, Kyoto, Japan.
T
T
Uemori
T
T
Shimojo
K
K
Asada
T
T
Asano
F
F
Kimizuka
I
I
Kato
M
M
Maki
M
M
Hatanaka
T
T
Murachi
H
H
Hanzawa
D000595:Amino Acid Sequence; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D002154:Calpain / Q000037:antagonists & inhibitors*; D002942:Circular Dichroism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D007700:Kinetics; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis; D010957:Plasmids; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000494:pharmacology; D015183:Restriction Mapping; D013728:Terminator Regions, Genetic
D002135:Calcium-Binding Proteins; D010455:Peptides; D011994:Recombinant Proteins; C033668:calpastatin; D002154:Calpain