[The role of the beta-subunit of RNA-polymerase in specific recognition of promoters].
The complex formation of T7 DNA with RNA polymerase from E. coli B/r WU-36-10-11-12 (E. coli W12) and its rifampicin resistant mutant with highly pleiotropic effect--rpoB409 was studied. As shown earlier rpoB409 RNA polymerase differs from the normal enzyme by the selection of RNA synthesis from early promoters of DNA from T7 and T4 phages. The change in the RNA specificity synthesis due to rpoB409 mutation was shown to occur at the stage of RNA polymerase interaction with DNA before open promoter complex formation. The data obtained together with the fact of highly pleiotropic effect of the rpoB409 mutation indicate that RNA polymerase beta-subunit takes part in specific recognition of promoters.
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D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D015246:Deoxyribonuclease EcoRI; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D009154:Mutation; D011401:Promoter Regions, Genetic; D011487:Protein Conformation; D012329:RNA, Bacterial / Q000096:biosynthesis; D013604:T-Phages / Q000235:genetics
D004279:DNA, Viral; D012329:RNA, Bacterial; D012321:DNA-Directed RNA Polymerases; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI
Rol' beta-sub''edinitsy RNK-polimerazy v spetsificheskom uznavanii promotorov.