Mitochondrial adenosine triphosphatase in mit- mutants of Saccharomyces cerevisiase with defective subunit 6 of the enzyme complex.
mit- Mutants carrying genetically defined mutations in the oli2 region of the mitochondrial DNA were analysed. Most of these mutants demonstrated either the absence of subunit 6 or its replacement by shorter mitochondrial translation products which could be shown to be structurally related to subunit 6 by using a rabbit anti F1F0-antiserum, and by limited proteolytic mapping of the new mitochondrial translation products. Three representative oli2 mit- strains were analysed for the effects of a grossly altered subunit 6 or of a complete absence of this subunit on the activity and assembly of the H+-ATPase. Our results suggest that this subunit is not required for the assembly of the proton channel of the enzyme complex. Thus, in the absence of subunit 6, the mitochondrial respiratory activities in the oli2 mutants were found to be still sensitive to oligomycin, a specific inhibitor of the H+-ATPase proton channel. Immunoprecipitation of the assembled H+-ATPase subunits from these mutant strains using a monoclonal anti-beta-subunit antibody indicates that subunit 6 is also not essential for the assembly of most F1 subunits to components of the F0 sector.
W M
WM
Choo
R G
RG
Hadikusumo
S
S
Marzuki
D004271:DNA, Fungal / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D007425:Intracellular Membranes / Q000201:enzymology; D007473:Ion Channels / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology*; D009097:Multienzyme Complexes / Q000235:genetics; D009154:Mutation; D009840:Oligomycins / Q000494:pharmacology; D010085:Oxidative Phosphorylation; D006180:Proton-Translocating ATPases / Q000096:biosynthesis / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics
D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D007473:Ion Channels; D009097:Multienzyme Complexes; D009840:Oligomycins; D006180:Proton-Translocating ATPases