Modulation of muscarinic-receptor expression in human embryonic lung fibroblasts by platelet-derived growth factor.
Platelet-derived growth factor (PDGF) is known to have regulatory control of a large number of cellular components, including various receptors. We show that muscarinic acetylcholine receptors of the m2 subtype on CCL 137 human fibroblasts in culture are affected by PDGF treatment. A time-dependent down-regulation is observed in steady-state RNA levels, followed by a decrease in ligand-binding capacity. Minimum RNA levels are attained at 11 h; minimum binding capacity is observed after 24 h of treatment. To our knowledge, this is the first example of negative gene control by PDGF.
Department of Biotechnology, Pasteur Institute, Paris, France.
A
A
Koman
O
O
Durieu-Trautmann
P O
PO
Couraud
A D
AD
Strosberg
B B
BB
Weksler
D002460:Cell Line; D015536:Down-Regulation; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007700:Kinetics; D008168:Lung; D009693:Nucleic Acid Hybridization; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D011813:Quinuclidinyl Benzilate / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011976:Receptors, Muscarinic / Q000235:genetics*
D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate