Microtubule-associated protein 1A is the fibroblast HMW MAP undergoing mitogen-stimulated serine phosphorylation.
A variety of antibodies to microtubule-associated protein (MAP) have been used to demonstrate that phosphorylation of a 350 kDa microtubule-associated protein is stimulated 2-3 fold by epidermal growth factor or serum in quiescent 3T3-L1 fibroblasts and by insulin in 3T3-L1 adipocytes. Phosphorylation occurs on serine residues, and is maximal by 15-20 min. The phosphoprotein has been identified as MAP1A by specific immunoprecipitation with a well-characterized monoclonal antibody.
Erickson
A K
AK
Department of Medicine, University of Virginia School of Medicine, Charlottesville 22908.
Ray
L B
LB
Sturgill
T W
TW
D000818:Animals; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005347:Fibroblasts; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008970:Molecular Weight; D010750:Phosphoproteins / Q000378:metabolism*; D010768:Phosphoserine / Q000378:metabolism; D011233:Precipitin Tests; D011494:Protein Kinases / Q000378:metabolism
D007328:Insulin; D008869:Microtubule-Associated Proteins; D010750:Phosphoproteins; D010768:Phosphoserine; D004815:Epidermal Growth Factor; D011494:Protein Kinases