Detection of bromodeoxyuridine-incorporation in mammalian chromosomes by a bromodeoxyuridine-antibody. I. Demonstration of replication patterns.
We have shown that a commercially available antibody allows the demonstration of bromodeoxyuridine (BrdU) incorporation into mammalian chromosomes by the typical immunologic staining techniques. The replication patterns obtained are comparable to those after BrdU-incorporation and FPG-staining, though with reversed staining specificity. The quality of the patterns equals that of commonly used differential staining but the sensitivity of BrdU-detection is superior. Thus the new technique may be very promising for all cytogenetic studies concerning any kind of chromosome replication.
Vogel
W
W
Autenrieth
M
M
Speit
G
G
D000818:Animals; D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine / Q000276:immunology / Q000378:metabolism*; D002478:Cells, Cultured; D002875:Chromosomes / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D004261:DNA Replication; D007621:Karyotyping; D008677:Metaphase; D013194:Staining and Labeling / Q000379:methods
D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine