Pituitary alpha-subunit messenger ribonucleic acid remains elevated during the latter stages of the preovulatory luteinizing hormone surge.
In this study, pituitary alpha-subunit mRNA amounts were assessed during the latter stages of the preovulatory LH surge in the normal cycling ewe. The times chosen were 15 and 25 h after the onset of behavioral estrus, designated E+15 and E+25. These mRNA values were correlated with pituitary and serum LH concentrations to assess the possible role of subunit synthesis during these times of the cycle, and then compared to values obtained from hybridizations of RNAs from animals in other groups representing defined stages of the cycle. One of these groups included animals during the midluteal phase (day 12), whose amounts were assigned a value of 1. It was observed that the amounts of alpha-subunit mRNA in E+15 and E+25 groups, as measured by translations and cDNA hybridizations, were approximately 10-fold higher than the day 12 values and were roughly similar to those observed in a group examined 5 h after estrus. Thus, it is evident that the alpha mRNA levels are not only elevated during the LH surge, but also remain high at a time when both serum and pituitary LH concentrations have fallen dramatically, suggesting the involvement of changing mRNA amounts in the feedback systems associated with the maintenance of pituitary and serum gonadotropin amounts.
Landefeld
T
T
Kaynard
A
A
Kepa
J
J
D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis; D004971:Estrus; D005260:Female; D005498:Follicular Phase; D015292:Glycoprotein Hormones, alpha Subunit; D007986:Luteinizing Hormone / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D009929:Organ Size; D010446:Peptide Fragments / Q000235:genetics*; D010902:Pituitary Gland / Q000032:analysis; D010908:Pituitary Hormones, Anterior / Q000235:genetics*; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis*; D012756:Sheep
D015292:Glycoprotein Hormones, alpha Subunit; D010446:Peptide Fragments; D010908:Pituitary Hormones, Anterior; D012333:RNA, Messenger; D007986:Luteinizing Hormone; D004247:DNA