Endocytosis of galactose-terminated glycoproteins by isolated liver cells of the rainbow trout (Salmo gairdneri).
Intravenously injected 125I-labeled galactose-terminated glycoproteins were mainly recovered in the liver of the rainbow trout. After injection of [14C]sucrose-labeled asialofetuin, the liver cells were isolated and separated by differential centrifugation. The radioactivity was located in the parenchymal cells. Uptake of asialoglycoproteins in liver cells was inhibited by EGTA, lactose and excess unlabeled ligand. Degradation was inhibited by ammonium chloride, suggesting a lysosomal process. Internalization of 125I-asialoglycoproteins was demonstrated by removing receptor-bound ligand with EGTA at different time points during the incubation. The cellular uptake occurred even at 0 degree C.
B H
BH
Dannevig
T
T
Berg
D000641:Ammonia / Q000494:pharmacology; D000818:Animals; D001212:Asialoglycoproteins; D004705:Endocytosis; D060748:Fetuins; D005690:Galactose; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D009961:Orosomucoid / Q000031:analogs & derivatives* / Q000378:metabolism; D014337:Trout; D000509:alpha-Fetoproteins / Q000378:metabolism*
D001212:Asialoglycoproteins; D060748:Fetuins; D009961:Orosomucoid; D000509:alpha-Fetoproteins; C017029:asialofetuin; C017030:asialoorosomucoid; D000641:Ammonia; D005690:Galactose