A study of insulin and glucagon secretion from adult rat pancreatic monolayer islets.
The present study confirms and extends previous observations that whole pancreatic islets form a monolayer culture in vitro. Our technique, using a medium containing 3-isobutyl-1-methylxanthine (IBMX), clearly demonstrated enzymatic disruption of the islets and cellular organization of isolated pancreatic islets. Insulin or glucagon secretion of monolayer culture was measured during incubation in a medium containing 5.5 mM D-glucose, then in 16.7 mM D-glucose, and finally in a combination of 16.7 mM D-glucose and IBMX, or of low glucose and 20 mM L-arginine. Clearly, such a technique might permit the recovery of collagenase-isolated pancreatic islets during the culture period and also an increase in glucose-induced insulin secretion and arginine stimulated glucagon secretion.
Ohgawara
H
H
Tasaka
Y
Y
Machiyama
E
E
Sakurai
Y
Y
Hirata
Y
Y
D015056:1-Methyl-3-isobutylxanthine; D000818:Animals; D001120:Arginine / Q000494:pharmacology; D003470:Culture Media; D046508:Culture Techniques; D005934:Glucagon / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D003012:Microbial Collagenase; D051381:Rats; D011919:Rats, Inbred Strains
D003470:Culture Media; D007328:Insulin; D005934:Glucagon; D001120:Arginine; D003012:Microbial Collagenase; D005947:Glucose; D015056:1-Methyl-3-isobutylxanthine