Investigation of T and natural killer cell function with monoclonal antibodies.
The relationship of T cell and natural killer (NK) cell antigens was examined using two monoclonal anti-T cell antibodies. The first--anti-HuLy-m1 (identical to OKTlla), reactive with all E-RFC+ cells and 50% null cells including NK cells--could partially block (greater than 50%) NK activity. The second--anti-HuLy-m2, reactive with T cells, some B cells, and null cells--could stimulate and not block NK activity. The enhancement of NK cell function by anti-HuLy-m2 was not related to interferon stimulation, and neither antibody altered effector:target cell binding. When used together neither augmentation nor blocking effects were observed, indicating different sites of reaction with NK cells. To further explore a possible association between T cell and NK antigens, anti-HuLy-m1 and -m2 were used to investigate certain T cell functions. Neither antibody was mitogenic for peripheral blood lymphocytes (PBLs) or blocked PHA stimulation of PBLs. In addition anti-HuLy-m1 did not inhibit a mixed lymphocyte culture (MLC) but could inhibit cytotoxic T lymphocytes (CTLs) almost as effectively as OKT3. However, anti-HuLy-m2 had no effect in an MLC and showed only minimal inhibition of a CTL assay. These studies demonstrate a relationship of the membrane structure of T cells and NK cells at an antigenic and functional level.
P J
PJ
Thurlow
G
G
McArthur
I F
IF
McKenzie
D000911:Antibodies, Monoclonal / Q000276:immunology*; D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic; D006801:Humans; D007372:Interferons / Q000032:analysis; D007694:Killer Cells, Natural / Q000276:immunology*; D004915:Leukemia, Erythroblastic, Acute / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects; D010835:Phytohemagglutinins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D010835:Phytohemagglutinins; D007372:Interferons