Influence of storage conditions on aluminum concentrations in serum, dialysis fluid, urine, and tap water.
The influence of storage temperature, vessel type, and treatment on alterations of aluminum (Al) concentrations in serum, urine, and dialysis fluid samples was studied at three different concentrations for each sample over an 18-month period. Furthermore, the influence of acidification on Al levels in tap water, urine, and dialysis fluid samples was studied over a four-month period. Al was measured by atomic absorption spectrometry. Sample storage in glass vessels was unsuitable, whereas only minor alterations of Al levels were observed with storage in polypropylene tubes, polystyrene tubes, and Monovettes. By using appropriate plastic containers, acid washing of the vessels showed no improvement. Frozen storage was superior compared with 4 degrees C, whereas storage at -80 degrees C offered no advantage compared with storage at -20 degrees C. Acidification of tap water samples was necessary to stabilize Al levels during storage. No striking effect of acidification on Al levels in urine and dialysis fluid samples was found. It is concluded that longterm storage of serum, urine, tap water, and dialysis fluid samples is possible if appropriate conditions are used.
Institute of Toxicology, Heinrich-Heine University of Düsseldorf, FRG.
M
M
Wilhelm
F K
FK
Ohnesorge
D000535:Aluminum / Q000032:analysis* / Q000097:blood / Q000652:urine; D015314:Dialysis Solutions / Q000032:analysis*; D005898:Glass; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010969:Plastics; D015203:Reproducibility of Results; D013048:Specimen Handling / Q000379:methods*; D013696:Temperature; D014873:Water Pollutants / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*
D015314:Dialysis Solutions; D010969:Plastics; D014873:Water Pollutants; D014874:Water Pollutants, Chemical; D000535:Aluminum