Cloning of cDNA and genomic DNA for human cytochrome P-45011 beta.
A full-length cDNA clone encoding steroid 11 beta-hydroxylase (P-45011 beta) has been isolated from a cDNA library derived from human adrenal tumor. The insert of the clone contains an open reading frame encoding a protein of 503 amino acid residues together with a 4 bp 5'-untranslated region and a 576 bp 3'-untranslated region to which a poly(A) tract is attached. The promoter region of the P-450(11) beta gene has also been isolated from a genomic library derived from human pre-B cells. It contains a TATA box, a putative cAMP-responsive element, several repeated sequences and two sequence elements similar to the consensus sequence for binding of AP-1. A transient expression assay in Y-1 adrenal tumor cells demonstrates that the promoter activity is remarkably enhanced by treatment of the cells with cAMP. In addition, analysis using deletion mutants containing various lengths of the 5'-flanking region of the gene suggests that several cis-acting elements participate in transcriptional regulation of human P-450(11) beta gene.
Department of Medical Chemistry, Kochi Medical School, Japan.
T
T
Kawamoto
Y
Y
Mitsuuchi
K
K
Toda
K
K
Miyahara
Y
Y
Yokoyama
K
K
Nakao
K
K
Hosoda
Y
Y
Yamamoto
H
H
Imura
Y
Y
Shizuta
D000310:Adrenal Gland Neoplasms / Q000201:enzymology / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000235:genetics* / Q000302:isolation & purification; D015870:Gene Expression; D015723:Gene Library; D005796:Genes; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D015183:Restriction Mapping; D013252:Steroid 11-beta-Hydroxylase / Q000235:genetics*; D013250:Steroid Hydroxylases / Q000235:genetics*
D004273:DNA, Neoplasm; D015345:Oligonucleotide Probes; D013250:Steroid Hydroxylases; D013252:Steroid 11-beta-Hydroxylase