A common ancestor for Candida tropicalis and dehydrogenases that synthesize antibiotics and steroids.
Candida tropicalis peroxisomes contain a 905-residue trifunctional enzyme with hydratase-dehydrogenase-epimerase activity that is important in fatty acid beta-oxidation. At its amino terminus are two tandem copies of an approximately 280 residue domain of unknown function. We provide evidence that this domain is homologous to oxidoreductases used for metabolizing sugars and synthesizing antibiotics and steroids such as estradiol, androstenedione, corticosterone, and hydrocortisone. The trifunctional enzyme shows no sequence similarity to the bifunctional hydratase-dehydrogenase found in animal peroxisomes and plant glyoxysomes, which are homologs of each other. We suggest that the C. tropicalis trifunctional enzyme and the animal and plant bifunctional enzymes have different ancestors.
Department of Medicine, University of California, San Diego, La Jolla 92093-0623.
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Baker
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000032:analysis / Q000145:classification / Q000235:genetics; D000595:Amino Acid Sequence; D002175:Candida / Q000201:enzymology*; D004746:Enoyl-CoA Hydratase / Q000032:analysis / Q000145:classification* / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D042843:Glucose 1-Dehydrogenase; D005948:Glucose Dehydrogenases / Q000032:analysis; D006836:Hydro-Lyases / Q000145:classification*; D007535:Isomerases / Q000145:classification*; D008830:Microbodies / Q000201:enzymology*; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000032:analysis / Q000145:classification* / Q000235:genetics; D063994:Peroxisomal Bifunctional Enzyme; D010802:Phylogeny; D019998:Racemases and Epimerases / Q000032:analysis / Q000145:classification* / Q000235:genetics
D009097:Multienzyme Complexes; D015094:3-Hydroxyacyl CoA Dehydrogenases; D005948:Glucose Dehydrogenases; D042843:Glucose 1-Dehydrogenase; D006836:Hydro-Lyases; D004746:Enoyl-CoA Hydratase; D063994:Peroxisomal Bifunctional Enzyme; D007535:Isomerases; D019998:Racemases and Epimerases