[Composition and properties of the outer cell wall layer in Bacillus brevis--the producer of gramicidin S].
Two membrane antigens were found by cross immunoelectrophoresis in the cell walls of Bacillus brevis var. G.-B., R form, which started to synthesize gramicidin S (20 mg per 1 ml of cultural broth). The cell wall contained no membrane components in cells at the beginning of the logarithmic growth phase. The protein with a molecular mass of 100 kDa is a component of the cell wall outer layer. The protein is not digested by trypsin or pronase when it comprises the cell walls of cells synthesizing gramicidin S. In the preparation of isolated cell walls, this protein becomes susceptible to the action of the above proteases only when the peptidoglycan layer is broken down by lysozyme. Electron microscopy of cells treated with proteases and shadowed with a metal revealed that many cells lacked the cytoplasm. Therefore, the outer layer of B. brevis R cell wall contains small regions susceptible to the action of protease along with regions composed of the 100 kDa protein and resistant to these enzymes. It is possible that the small regions contain membrane components.
Simakova
I M
IM
Kharat'ian
E F
EF
Vostroknutova
G N
GN
Bulgakova
V G
VG
Udalova
T P
TP
D000942:Antigens, Bacterial / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D001407:Bacillus / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D001426:Bacterial Proteins / Q000032:analysis; D002473:Cell Wall / Q000032:analysis / Q000187:drug effects / Q000276:immunology; D003378:Counterimmunoelectrophoresis / Q000379:methods; D006096:Gramicidin / Q000096:biosynthesis*; D008837:Micrococcus / Q000187:drug effects; D010447:Peptide Hydrolases / Q000494:pharmacology; D010740:Phospholipases / Q000302:isolation & purification / Q000494:pharmacology
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001426:Bacterial Proteins; D006096:Gramicidin; D010740:Phospholipases; D010447:Peptide Hydrolases
Sostav i svoĭstva naruzhnogo sloia kletochnoĭ stenki Bacillus brevis--produtsenta gramitsidina S.