Beta-adrenergic stimulation of mouse parotid gland: amylase activity and secretory granules during isoproterenol-induced mitosis.
This paper examines the mitotic activity of parotid glands of groups of mice sacrificed at 1 hr-intervals between 26 and 36 hr after being injected with dl-isoproterenol (IPR, 1 mumol/g, single dose, i.p.). Maximal mitotic activity occurs 35 hr after IPR injection; there is a concomitant rise of amylase activity (64% over control). Mitotic cells showed increased number of secretory granules (N) with regard to interphasic cells. However, the increment of N is paralleled by an increment of the cell section area (A); the N/A ratio is thus maintained around 0.45 throughout. These findings suggest that during IPR-induced mitosis of mice parotid cells there is not a blockade of the biosynthetic pathways which lead to the appearance of secretory granules in the cell cytoplasm.
Chapola
A
A
Villa
N
N
Valle
L B
LB
Oliveira-Filho
R M
RM
Minetti
C A
CA
D000681:Amylases / Q000378:metabolism*; D000818:Animals; D003594:Cytoplasmic Granules / Q000187:drug effects / Q000648:ultrastructure*; D007399:Interphase; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008938:Mitosis / Q000187:drug effects*; D010306:Parotid Gland / Q000201:enzymology* / Q000648:ultrastructure
D000681:Amylases; D007545:Isoproterenol