Vascular and adrenal receptors for atrial natriuretic factor in the rat.
Previous studies have shown that atrial natriuretic factor, a powerful vasorelaxant of precontracted vessels, inhibits the secretion of aldosterone stimulated by angiotensin II, adrenocorticotropic hormone, and potassium. We now report the presence of specific binding sites for atrial natriuretic factor in rat blood vessels (mesenteric and renal arteries) and adrenal capsules. Radioiodinated synthetic atrial natriuretic factor bound to a single class of high-affinity (KD = 0.1 nM) low-capacity receptors in a particulate fraction from blood vessels and adrenals. Unrelated peptides did not displace atrial natriuretic factor. Fragments of atrial natriuretic factor displaced the labeled ligand with decreasing potency after cleavage at the N-terminal. The cleavage of the C-terminal tyrosine did not decrease the potency of atrial natriuretic factor, but further cleavage at the C-terminal dramatically reduced the affinity of the resulting peptides. The potency of the atrial natriuretic factor fragments in the radioligand assay was in proportion to their potency to inhibit aldosterone secretion by isolated rat glomerulosa cells. Our results suggest that these binding sites mediate the biological actions of atrial natriuretic factor in blood vessels and the adrenal, and that both receptors have similar specificities.
Schiffrin
E L
EL
Chartier
L
L
Thibault
G
G
St-Louis
J
J
Cantin
M
M
Genest
J
J
D000311:Adrenal Glands / Q000378:metabolism*; D000450:Aldosterone / Q000096:biosynthesis; D000595:Amino Acid Sequence; D000818:Animals; D009320:Atrial Natriuretic Factor; D001667:Binding, Competitive; D001808:Blood Vessels / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000378:metabolism; D007473:Ion Channels / Q000378:metabolism*; D007700:Kinetics; D008566:Membranes / Q000378:metabolism; D009124:Muscle Proteins / Q000378:metabolism*; D009318:Natriuresis; D051381:Rats; D017461:Receptors, Atrial Natriuretic Factor; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013329:Structure-Activity Relationship
D002121:Calcium Channel Blockers; D007473:Ion Channels; D009124:Muscle Proteins; D011956:Receptors, Cell Surface; C472610:atrial natriuretic peptide, rat; D000450:Aldosterone; D009320:Atrial Natriuretic Factor; D017461:Receptors, Atrial Natriuretic Factor