Cellular proliferation in the anterior pituitary of the rat during the postnatal period.
Cellular proliferation in the anterior pituitary of 2-, 8-, 15- and 30-day-old rats was examined by injection of bromodeoxyuridine 1 h before autopsy. Bromodeoxyuridine incorporated into DNA was detected immunohistochemically by use of a monoclonal antibody. The highest rate of cell proliferation was found in 2-day-old animals; it decreased thereafter during the postnatal period. Possible toxic effects of colchicine on cellular proliferation were examined. Colchicine treatment (10 mg/kg in 8- and 30-day-old animals) significantly decreased the number of bromodeoxyuridine-labelled cells/mm2 in 8-day-old rats. Some sections were doubly immunostained for bromodeoxyuridine and various pituitary hormones. The proportion of doubly-immunostained cells to all proliferating cells was generally low, ranging from 23% at 2 days to 32% at 30 days of age.
Institute of Biology, Faculty of Science, Niigata University, Japan.
E
E
Carbajo-Pérez
Y G
YG
Watanabe
D000818:Animals; D001973:Bromodeoxyuridine; D002455:Cell Division / Q000187:drug effects; D003078:Colchicine / Q000494:pharmacology; D004247:DNA / Q000032:analysis; D004261:DNA Replication; D005260:Female; D008297:Male; D008940:Mitotic Index; D010903:Pituitary Gland, Anterior / Q000166:cytology* / Q000254:growth & development; D010908:Pituitary Hormones, Anterior / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D009418:S100 Proteins / Q000032:analysis
D010908:Pituitary Hormones, Anterior; D009418:S100 Proteins; D004247:DNA; D001973:Bromodeoxyuridine; D003078:Colchicine