Further characterization of internal image-bearing anti-idiotypic antibodies: specific binding to immunoglobulin receptors on murine hybridoma cells secreting antibodies to hepatitis B surface antigen.
The further characterization of internal image anti-idiotypic antibodies (anti-Id) that represent a potential alternative vaccine candidate for type B viral hepatitis is described. The anti-Id preparation contains an internal image component or related epitope that mimics hepatitis B surface antigen (HBsAg) and binds to murine hybridoma cells that secrete antibodies to HBsAg (anti-HBs). This binding to anti-HBs-secreting hybridomas was partially inhibited by intact HBsAg particles and was associated with the expression of an interspecies idiotype. Immunoprecipitation studies demonstrated that the anti-Id bound to immunoglobulin molecules expressed on the surface of the hybridoma cells. These data suggest that internal image anti-Id, which induces an in vivo antibody response by antigenic mimicry in the absence of HBsAg, binds to anti-HBs molecules on the surface of cells actively secreting anti-HBs. The possible mechanism for internal image anti-Id-based antibody vaccines that mimic the overall conformation of antigens associated with infectious agents is discussed.
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RC
Kennedy
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Henkel
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GR
Dreesman
D000818:Animals; D001667:Binding, Competitive; D000939:Epitopes; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D051379:Mice; D011487:Protein Conformation; D011947:Receptors, Antigen, B-Cell / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology
D000939:Epitopes; D006514:Hepatitis B Surface Antigens; D007130:Immunoglobulin Idiotypes; D011947:Receptors, Antigen, B-Cell; D014765:Viral Vaccines