Neurotransmitter receptor autoradiography in immunohistochemically identified neurons.
A method for the simultaneous visualization of neurotransmitter receptor binding sites and immunohistochemically characterized cells is described using in vitro sections of rat striatum. The striatum provides a rich neurochemical environment in which to examine muscarinic acetylcholine receptor relations to peptide-reactive somata, or to assess the cellular locale of the dopamine receptor linked to adenylate cyclase activation. Different modifications of the procedure are employed to determine the localization pattern of 3H-radioligands with slow or rapid receptor dissociation times. The present technique is compatible for use with any combination of immunohistochemical antibody/radioligand probe that can be studied on fresh-frozen, slide-mounted tissue sections.
Ariano
M A
MA
Kenny
S L
SL
D000818:Animals; D001345:Autoradiography; D003342:Corpus Striatum / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D007124:Immunoenzyme Techniques; D051381:Rats; D011919:Rats, Inbred Strains; D011954:Receptors, Dopamine / Q000378:metabolism; D017447:Receptors, Dopamine D1; D011976:Receptors, Muscarinic / Q000378:metabolism; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D013373:Substance P / Q000378:metabolism
D011954:Receptors, Dopamine; D017447:Receptors, Dopamine D1; D011976:Receptors, Muscarinic; D017981:Receptors, Neurotransmitter; D013373:Substance P; D000242:Cyclic AMP