Possible relationship between chromosome alterations and in vitro cellular radiosensitivity of human malignant melanoma.
In the present study, in vitro radiation survival analysis was performed on 8 human malignant melanoma cell lines with defined karyotypes. Exponentially growing cells were irradiated to doses of 0 to 8 Gy and examined for soft agar clonogenicity. Two groups emerged from this analysis: 4 cell lines with a small shoulder (extrapolation number, n less than 2) and 4 cell lines with a large shoulder (n greater than 2). Of possible significance, 4/4 cell lines with extrapolation numbers greater than 2 demonstrated clonal structural abnormalities of chromosome 7. In contrast, 3/4 cell lines with extrapolation numbers less than 2 had no structural abnormalities of chromosome 7. These very preliminary results suggest that structural alterations of chromosome 7 may be associated with melanoma tumors with high extrapolation numbers.
Dept. of Radiation Oncology, University of Arizona, Arizona Cancer Center, Tucson 85724.
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H
McBride
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Stea
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Hill
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Trent
D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D002869:Chromosome Aberrations; D002875:Chromosomes; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D066298:In Vitro Techniques; D008545:Melanoma / Q000235:genetics / Q000473:pathology / Q000503:physiopathology*; D011836:Radiation Tolerance