Localization of cerium-based reaction products by scanning laser reflectance confocal microscopy.
Scanning laser confocal microscopy was utilized to visualize sites of hydrogen peroxide release from stimulated neutrophils and lysosomal acid phosphatase in these and other cells using cerium in the detection systems. Imaging of the cerium-containing reactions was achieved by employing the reflectance mode of this instrument. Localization of these products at the light microscope level was direct and did not require other reactions to generate a visible product. This new approach to cerium cytochemistry should prove useful for many applications.
Department of Anatomy, Ohio State University, Columbus 43210.
J M
JM
Robinson
B E
BE
Batten
D000135:Acid Phosphatase / Q000032:analysis*; D000818:Animals; D002563:Cerium; D005260:Female; D006107:Granulosa Cells / Q000032:analysis; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis*; D007089:Image Enhancement; D007834:Lasers; D051379:Mice; D008858:Microscopy, Phase-Contrast; D009504:Neutrophils / Q000032:analysis* / Q000187:drug effects / Q000648:ultrastructure; D010587:Phagocytosis
D002563:Cerium; D006861:Hydrogen Peroxide; D000135:Acid Phosphatase; C026690:cerous chloride