Calmodulin in interferon preparations: effect of interferon on calmodulin bioactivity.
Heat-stable calmodulin immunoreactivity and bioactivity were detected in crude preparations of various types of human, murine and chicken interferons (IFNs). Calmodulin containing HuIFN-alpha was retained on a trifluorophenothiazine-Sepharose column. The two activities were separated by serial elutions with 50 microM Ca2+ (HuIFN-alpha) followed by 2 mM EGTA (calmodulin). While maintaining its full antiviral activity, calmodulin free HuIFN-alpha inhibited enhancement of Ca2+-ATPase activity in vitro by authentic purified eukaryote calmodulin. These results indicate that IFNs are calmodulin-binding proteins and that the secretion of both IFNs and calmodulin occurs from IFN-induced cells.
Davis
P J
PJ
Thacore
H R
HR
Chadha
K C
KC
Lawrence
W D
WD
Kite
J H
JH
D000818:Animals; D002147:Calmodulin / Q000032:analysis* / Q000235:genetics; D002642:Chick Embryo; D002846:Chromatography, Affinity; D006801:Humans; D007372:Interferons / Q000032:analysis* / Q000235:genetics / Q000494:pharmacology; D051379:Mice; D010640:Phenothiazines
D002147:Calmodulin; D010640:Phenothiazines; D007372:Interferons