alpha-Fetoprotein synthesis in transformed fetal rat liver cells.
We have reported that transformed fetal liver cells produced a variant alpha-fetoprotein of 65K that differed from the mature alpha-fetoprotein of 69K and 73K in the polypeptide backbone. In the present study, we demonstrated that the biosynthetic pathway of the variant alpha-fetoprotein differed from that of the mature alpha-fetoprotein. The 65K variant was synthesized first as a preprotein of 49.5K which was processed to a polypeptide of 59K in the presence of microsomal membranes. The latter was the precursor of the variant alpha-fetoprotein found in cells and medium of transformed fetal liver cells. The 65K alpha-fetoprotein was encoded by a mRNA of 16S while mature AFP was encoded by a mRNA of 20S.
Chou
J Y
JY
Savitz
A J
AJ
D000818:Animals; D002472:Cell Transformation, Viral; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000096:biosynthesis; D008970:Molecular Weight; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D013539:Simian virus 40; D014158:Transcription, Genetic; D000509:alpha-Fetoproteins / Q000096:biosynthesis* / Q000235:genetics
D006023:Glycoproteins; D012333:RNA, Messenger; D000509:alpha-Fetoproteins