DNA amplification of HIV-1 in seropositive individuals and in seronegative at-risk individuals.
We assessed the HIV-1 status of seropositive and seronegative at-risk individuals by the polymerase chain reaction. Fifty-four out of 55 HIV-1-seropositive samples scored positive. However, HIV-1 proviral DNA was not detected in 16 seronegative homosexuals, 20 seronegative polytransfused haemophiliacs and 20 seronegative thalassaemic children, 20 individuals with isolated and persistent anti-core antibodies and 74 seronegative blood donors. These data indicate that positive HIV-1 DNA is likely to be an exceptional phenomenon in HIV-seronegative people.
Institut National de Transfusion Sanguine, Université Pierre et Marie Curie, Paris, France.
M
M
Mariotti
J J
JJ
Lefrère
B
B
Noel
F
F
Ferrer-Le-Coeur
D
D
Vittecoq
R
R
Girot
C
C
Bosser
A M
AM
Couroucé
C
C
Salmon
P
P
Rouger
D000328:Adult; D001483:Base Sequence; D002648:Child; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D005784:Gene Amplification; D006679:HIV Seropositivity / Q000235:genetics / Q000382:microbiology*; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012307:Risk Factors
D015342:DNA Probes; D004279:DNA, Viral