Demonstration of immunochemical identity between the nerve growth factor-inducible large external (NILE) glycoprotein and the cell adhesion molecule L1.
The nerve growth factor-inducible large external (NILE) glycoprotein and the neural cell adhesion molecule L1 were shown to be immunochemically identical. Immunoprecipitation with L1 and NILE antibodies of [3H]fucose-labeled material from culture supernatants and detergent extracts of NGF-treated rat PC12 pheochromocytoma cells yielded comigrating bands by SDS-PAGE. NILE antibodies reacted with immunopurified L1 antigen, but not with N-CAM and other L2 epitope-bearing glycoproteins from adult mouse brain. Finally, by sequential immunoprecipitation from detergent extracts of [35S]methionine-labeled early post-natal cerebellar cell cultures or [3H]fucose-labeled NGF-treated PC12 cells, all immunoreactivity for NILE antibody could be removed by pre-clearing with L1 antibody and vice versa.
Bock
E
E
Richter-Landsberg
C
C
Faissner
A
A
Schachner
M
M
D000310:Adrenal Gland Neoplasms / Q000032:analysis*; D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D000954:Antigens, Surface / Q000032:analysis*; D015815:Cell Adhesion Molecules; D002460:Cell Line; D002478:Cells, Cultured; D002531:Cerebellum / Q000032:analysis*; D000939:Epitopes / Q000032:analysis; D005643:Fucose / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis*; D007118:Immunoassay; D008715:Methionine / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D009414:Nerve Growth Factors / Q000494:pharmacology*; D039842:Neural Cell Adhesion Molecule L1; D010673:Pheochromocytoma / Q000032:analysis*; D051381:Rats; D013462:Sulfur Radioisotopes; D014316:Tritium
D000906:Antibodies; D000936:Antigen-Antibody Complex; D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D000939:Epitopes; D006023:Glycoproteins; D009414:Nerve Growth Factors; D039842:Neural Cell Adhesion Molecule L1; D013462:Sulfur Radioisotopes; D014316:Tritium; D005643:Fucose; D008715:Methionine