In vivo splicing of the premRNAs from early region 3 of adenovirus-2: the products of cleavage at the 5' splice site of the common intron.
The nuclear transcripts of the early region 3 from adenovirus-2 were studied for the presence of the cleavage products of premRNA at the 5' splice site of the first intervening sequence. Two molecules, free exon 1 and intron-exon 2-poly(A) were characterized by complementary methods including Northern blotting, RNase and S1 nuclease mapping, hybrid-selection and primer extension. The intron of the intron-exon 2 molecule is at least partially in a lariat form. A study of the time course of appearance of the 2 molecules shows that their formation occurs after the synthesis of premRNA, the lag period being no more than 1-2 min and before the formation of mature mRNA which starts accumulating after 3-4 min. These data are compatible with the idea that the 5' cleavage products are splicing intermediates in vivo.
Sittler
A
A
Gallinaro
H
H
Jacob
M
M
D000260:Adenoviruses, Human / Q000235:genetics*; D002467:Cell Nucleus / Q000378:metabolism; D004720:Endonucleases; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D009694:Nucleic Acid Precursors / Q000235:genetics*; D011061:Poly A / Q000235:genetics; D012313:RNA / Q000235:genetics; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D012260:Ribonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014158:Transcription, Genetic
D009694:Nucleic Acid Precursors; D012333:RNA, Messenger; D012367:RNA, Viral; D011061:Poly A; D012313:RNA; D004720:Endonucleases; D012260:Ribonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases