Intragranular colocalization of CRF and Met-Enk-8 in nerve terminals in the rat median eminence.
The ultrastructural localizations of rat corticoliberin (rCRF) and methionine-enkephalin octapeptide (Met-Enk-8) in the external layer of the rat median eminence were examined by double-immunogold labeling with anti-rCRF and anti-Met-Enk-8 sera labeled with small-sized (Gs) and medium-sized (Gm) gold particles, respectively. Two types of immunolabeled terminals were distinguished: one type with small granules (70 nm) labeled with Gs, and the other with large granules (100 nm) labeled with Gm. In both types, however, some granules were labeled with both Gs and Gm.
Hisano
S
S
Daikoku
S
S
Yanaihara
N
N
Shibasaki
T
T
D000818:Animals; D000936:Antigen-Antibody Complex; D003346:Corticotropin-Releasing Hormone / Q000032:analysis*; D003594:Cytoplasmic Granules / Q000648:ultrastructure*; D004744:Enkephalin, Methionine / Q000031:analogs & derivatives* / Q000032:analysis; D007106:Immune Sera; D008297:Male; D008473:Median Eminence / Q000166:cytology / Q000648:ultrastructure*; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D004744:Enkephalin, Methionine; C033453:enkephalin-Met, Arg(6)-Gly(7)-Leu(8)-; D003346:Corticotropin-Releasing Hormone