Comparison of lectin binding patterns in salivary glands of mice and rats with special reference to different fixatives used.
Lectin binding patterns in the salivary glands of mice and rats were compared among specimens treated with 9 kinds of fixatives and then individually subjected to 10 kinds of lectin staining. Formalin-fixed sections showed positive lectin binding with fine granular materials in the GCT cells, and alcohol- or acetone-fixed sections revealed vacuolated patterns of irregular lectin binding with insufficient morphologic detail. Bouin's, Helly's, and Zamboni's fixatives displayed an adequate distribution of lectin binding which corresponded to the histological aspects. Different lectins gave different characteristic binding patterns in SMGs of mice; i.e., PNA and SBA binding was positive in female GCT cells, but absent in the male. On the contrary these lectins gave positive binding in the male acinar cells but negative in the female. These contradictory results were obtained for PNA and SBA binding between the GCT cells and acinar compartments of the mouse SMGs. The GCT and acinar cells in the SMGs of mice and rats also gave contradictory results; i.e., mice GCT cells displayed positive Con A staining but negative PA/Con A staining, whereas mice acinar cells were stained weakly by the Con A and staining strongly by the PA/Con A methods. Rats GCT cells indicated negative Con A, and strong PA/Con A staining; whereas rats acinar cells gave a positive Con A and negative PA/Con A reaction.
Takai
Y
Y
Murase
N
N
Hosaka
M
M
Sumitomo
S
S
Noda
Y
Y
Mori
M
M
D000818:Animals; D005260:Female; D005404:Fixatives; D037102:Lectins / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D019887:Peanut Agglutinin; D037121:Plant Lectins; D051381:Rats; D011919:Rats, Inbred Strains; D011952:Receptors, Concanavalin A / Q000032:analysis; D011975:Receptors, Mitogen / Q000032:analysis*; D012469:Salivary Glands / Q000032:analysis* / Q000166:cytology / Q000378:metabolism; D012727:Sex Characteristics; D030262:Soybean Proteins; D013194:Staining and Labeling / Q000379:methods; D014909:Wheat Germ Agglutinins; D010835:Phytohemagglutinins
D005404:Fixatives; D037102:Lectins; D019887:Peanut Agglutinin; D037121:Plant Lectins; D011952:Receptors, Concanavalin A; D011975:Receptors, Mitogen; D030262:Soybean Proteins; D014909:Wheat Germ Agglutinins; C036554:dolichos biflorus agglutinin; C036292:pea lectin; C028824:soybean lectin; D010835:Phytohemagglutinins