Mechanisms involved in retinal pigment epithelial cell chemotaxis.
Retinal pigment epithelial (RPE) cell chemotaxis may play an important role in proliferative vitreoretinopathy (PVR). Fibronectin and platelet-derived growth factor are chemoattractants for RPE cells. In this study, we used these chemoattractants to examine mechanisms involved in RPE cell chemotaxis. Our results demonstrate that inhibition of RNA and protein synthesis, but not DNA synthesis, are associated with reduced chemotaxis. Microtubules and microfilaments are involved since cytochalasin B and colchicine are potent inhibitors of RPE cell migration. Dexamethasone, to which a beneficial effect has been attributed in an animal model of PVR, had no effect on RPE cell migration. Information concerning mechanisms involved in RPE cell migration may help to explore new avenues of treatment in PVR.
P A
PA
Campochiaro
B M
BM
Glaser
D001696:Biomechanical Phenomena; D002465:Cell Movement / Q000187:drug effects; D002633:Chemotaxis / Q000187:drug effects; D003078:Colchicine / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D003599:Cytoskeleton / Q000502:physiology; D004247:DNA / Q000502:physiology; D003907:Dexamethasone / Q000494:pharmacology; D005128:Eye Diseases / Q000209:etiology; D005136:Eye Proteins / Q000096:biosynthesis; D005472:Fluorouracil / Q000494:pharmacology; D006801:Humans; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000502:physiology*; D012313:RNA / Q000502:physiology; D012164:Retinal Diseases / Q000209:etiology; D014822:Vitreous Body
D005136:Eye Proteins; D003571:Cytochalasin B; D012313:RNA; D003907:Dexamethasone; D004247:DNA; D003078:Colchicine; D005472:Fluorouracil