Enhanced activity of immobilized dimethylmaleic anhydride-protected poly- and monoclonal antibodies.
The effect of reversible protection of the free amino groups of poly- and monoclonal antibodies by dimethylmaleic anhydride on their binding activity following immobilization onto various carriers was studied. The treatment with dimethylmaleic anhydride resulted in a 1.6-1.8-fold increase in the activity of immobilized goat anti-mouse immunoglobulin antibody immobilized onto different epoxy containing carriers and a 3-10.7-fold increase in the activity of immobilized monoclonal antibodies specific for carboxypeptidase A. The increase in activity was most pronounced at low antigen to carrier loads and over a wide range of modifier to protein ratios. The application of reversible protection of antibodies may permit the development of highly active immobilized antibody preparations for use in immunoaffinity purification.
Department of Biotechnology, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Israel.
E
E
Hadas
R
R
Koppel
F
F
Schwartz
O
O
Raviv
G
G
Fleminger
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D008024:Ligands; D008299:Maleic Anhydrides; D051379:Mice; D008815:Mice, Inbred Strains; D012685:Sepharose
D000906:Antibodies; D000911:Antibodies, Monoclonal; D008024:Ligands; D008299:Maleic Anhydrides; C007474:2,3-dimethylmaleic anhydride; D012685:Sepharose