Cytochalasin B induces cellular DNA fragmentation.
Cellular DNA fragmentation can be induced in many biological instances without plasma membrane damage. The fungal metabolite, cytochalasin B, is capable of modifying numerous cellular functions related to DNA synthesis. In this work it is demonstrated that cytochalasin B is capable of inducing DNA fragmentation in a number of cells lines. This DNA fragmentation occurs before plasma membrane lysis and over a period of hours. Cytochalasin E and villin, agents that act on the microfilaments, also induce DNA fragmentation. Phorbol dibutyrate, a diacylglyceral analog, is able to inhibit cytochalasin B-induced DNA fragmentation in a dose-dependent fashion. These findings support the interpretation that cytochalasin B is inducing DNA fragmentation via its effect on the actin filaments.
Department of Medicine, University of Miami School of Medicine, Florida 33101.
M A
MA
Kolber
K O
KO
Broschat
B
B
Landa-Gonzalez
D000199:Actins / Q000378:metabolism; D000818:Animals; D002352:Carrier Proteins / Q000633:toxicity; D002460:Cell Line; D002463:Cell Membrane Permeability; D002467:Cell Nucleus / Q000187:drug effects; D003571:Cytochalasin B / Q000037:antagonists & inhibitors / Q000633:toxicity*; D003572:Cytochalasins / Q000378:metabolism / Q000633:toxicity; D004247:DNA / Q000187:drug effects* / Q000378:metabolism; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004587:Electrophoresis, Agar Gel; D006801:Humans; D008840:Microfilament Proteins / Q000633:toxicity; D008970:Molecular Weight; D010703:Phorbol Esters / Q000494:pharmacology; D051381:Rats; D013997:Time Factors
D000199:Actins; D002352:Carrier Proteins; D003572:Cytochalasins; D008840:Microfilament Proteins; D010703:Phorbol Esters; C026615:villin; C007215:cytochalasin E; D003571:Cytochalasin B; D004247:DNA