Monoclonal 'internal image' anti-idiotypic antibodies of hepatitis B surface antigen.
The hypervariable regions of the immunoglobulin molecule which function as the antigen-combining site are, themselves, capable of provoking an antibody response. These antigenic determinants on the immunoglobulin are termed the 'idiotype', and antibodies directed against them 'anti-idiotype'. In circumstances where there is a close complementarity of shape between antigen and idiotype, and subsequently between idiotype and anti-idiotype, it would be predicted that anti-idiotype would be like an 'internal image' of the antigen. Starting with a monoclonal antibody (idiotype) to the protective a determinant of the hepatitis B surface antigen (HBsAg), we have succeeded in raising two monoclonal anti-idiotypes which mimic HBsAg in their ability to bind polyclonal antibodies to HBsAg produced in a variety of species. These internal image anti-idiotypes may provide a strategy for immunization without the need for antigen.
Y M
YM
Thanavala
A
A
Bond
R
R
Tedder
F C
FC
Hay
I M
IM
Roitt
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis*; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001667:Binding, Competitive; D000939:Epitopes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000939:Epitopes; D006514:Hepatitis B Surface Antigens; D007106:Immune Sera; D007130:Immunoglobulin Idiotypes