Mitogen-activated lymphocytes of normals and glioma patients produce factors with anti-glioblastoma activity in vitro.
Human glioblastoma cell lines showed profound suppression of both DNA and RNA synthesis when exposed to supernatants (SNs) of mitogen-activated blood mononuclear cells. Cloning efficiency of these glioma cells also decreased 10- to 500-fold. In monolayer cultures, growth inhibition was evident within 12 h of adding SN and peaked at 24 h. A decrease in absolute cell number was evident by 72 h. The inhibitory effect of SNs, however, was not permanent as more cells entered S-phase when SN-treated cultures were refed with fresh medium (without SN). The factor(s) responsible for this inhibitory activity was a product of lymphocytes and was produced in comparable amounts by cells of normal blood donors and patients with glioma. The compromised immunological status of glioblastoma patients did not influence their capacity to produce cytostatic lymphokines.
Merchant
R E
RE
Fontana
A
A
Nelson
A T
AT
Young
H F
HF
D000328:Adult; D000368:Aged; D000818:Animals; D002460:Cell Line; D003124:Colorimetry; D003208:Concanavalin A / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D005260:Female; D005909:Glioblastoma / Q000503:physiopathology*; D005910:Glioma / Q000503:physiopathology; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology*; D008222:Lymphokines / Q000096:biosynthesis / Q000302:isolation & purification; D008297:Male; D008875:Middle Aged; D010835:Phytohemagglutinins / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis; D051381:Rats
D008222:Lymphokines; D010835:Phytohemagglutinins; C044162:leukoregulin; C028784:phytohemagglutinin-P; D003208:Concanavalin A; D012313:RNA; D004247:DNA