Chromatographic evidence for high-molecular-mass galanin immunoreactivity in pig and cat adrenal glands.
Galanin was measured by radioimmunoassay in extracts of pig, cat and rat adrenals using non-C- and mid to C-terminally directed antibodies. The extracts were fractioned by gel chromatography and HPLC. The non-C-terminal galanin immunoreactivity in pig was 92.8 +/- 11.7 pmol/g, in cat 9.1 +/- 0.9 pmol/g and in rat less than 1 pmol/g. Two higher molecular forms of galanin have been identified in both pig and cat adrenal. One major large form behaves as if it was N-terminally extended (Kav pig 0.58, cat 0.48) and the other, a very high-molecular-mass form (Kav pig 0.10, 0.24, cat 0.10), as if it had both N- and C-terminal extensions.
F E
FE
Bauer
T E
TE
Adrian
N
N
Yanaihara
J M
JM
Polak
S R
SR
Bloom
D000311:Adrenal Glands / Q000032:analysis*; D000818:Animals; D002415:Cats; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D019004:Galanin; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis*; D011863:Radioimmunoassay; D051381:Rats; D013552:Swine
D010455:Peptides; D019004:Galanin