A novel in vitro assay for mouse IgE.
A simple and sensitive assay for screening mouse IgE-producing hybridomas is described. Rat basophil leukemia (RBL) cells (either live or glutaraldehyde fixed) were sensitized by mouse monoclonal IgE and then rosetted with antigen-coated sheep red blood cells. The assay was established using a mouse monoclonal IgE anti-DNP and was able to detect as little as 5 ng/ml IgE. The rosette assay is also a useful technique for studying the binding of mouse IgG monoclonal (MC) antibodies to RBL cells.
T J
TJ
Hall
M B
MB
Rittenberg
D000818:Animals; D001491:Basophils / Q000276:immunology; D000939:Epitopes; D006825:Hybridomas / Q000032:analysis; D007073:Immunoglobulin E / Q000032:analysis*; D007942:Leukemia, Experimental / Q000276:immunology; D051379:Mice / Q000276:immunology*; D051381:Rats; D012397:Rosette Formation; D013997:Time Factors
D000939:Epitopes; D007073:Immunoglobulin E