Human cytomegalovirus polypeptides stimulate neutralizing antibody in vivo.
At least three human cytomegalovirus polypeptides are targets for virus neutralizing antibody; a single protein of 86,000 molecular weight (p86) and two coimmunoprecipitating proteins of 130,000 and 55,000 molecular weight (p130/55). These polypeptides have been isolated by immunoaffinity chromatography and tested for immunogenicity in guinea pigs. Neutralizing antibody was detected after immunization with both p86 and p130/55. Hyperimmune sera to p130/55, but not p86, were dependent upon guinea pig complement for virus neutralization.
Rasmussen
L
L
Mullenax
J
J
Nelson
M
M
Merigan
T C
TC
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D003165:Complement System Proteins / Q000276:immunology; D003587:Cytomegalovirus / Q000276:immunology*; D000939:Epitopes; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D007114:Immunization; D008970:Molecular Weight; D009500:Neutralization Tests; D010455:Peptides / Q000276:immunology / Q000302:isolation & purification; D014764:Viral Proteins / Q000276:immunology* / Q000302:isolation & purification
D000914:Antibodies, Viral; D000939:Epitopes; D010455:Peptides; D014764:Viral Proteins; D003165:Complement System Proteins