Effects of K+ concentration on inhibition of protein synthesis by trimer and tetramer triphosphates of 2',5'-oligoadenylates in rabbit reticulocyte lysates.
The inhibition of protein synthesis by trimer or tetramer triphosphates of 2',5'-oligoadenylates (2-5A) in rabbit reticulocyte lysates is dependent on the concentration of K+ in the assay mixture. Based on studies using mRNA-dependent rabbit reticulocyte lysates and the degradation of [3H] polyadenylated mRNA, the greater inhibitory effects of trimer or tetramer triphosphates at the higher K+ is shown to be due to a more active 2-5A-dependent endoribonuclease (RNase L).
Wu
J M
JM
Konno
S
S
Eslami
B
B
D000227:Adenine Nucleotides / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D004722:Endoribonucleases / Q000378:metabolism; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics / Q000378:metabolism; D009841:Oligonucleotides / Q000494:pharmacology*; D009843:Oligoribonucleotides / Q000494:pharmacology*; D011061:Poly A / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger; D011817:Rabbits; D012156:Reticulocytes / Q000187:drug effects / Q000378:metabolism*
D000227:Adenine Nucleotides; D009841:Oligonucleotides; D009843:Oligoribonucleotides; D012333:RNA, Messenger; D011061:Poly A; C023505:2',5'-oligoadenylate; D012313:RNA; D004722:Endoribonucleases; C031956:2-5A-dependent ribonuclease; D011188:Potassium