Ursodeoxycholic acid, chenodeoxycholic acid, and 7-ketolithocholic acid are primary bile acids of the guinea pig.
Guinea pig gallbladder bile contains chenodeoxycholic acid (62 +/- 5%), ursodeoxycholic acid (8 +/- 5%), and 7-ketolithocholic acid (30 +/- 5%). All three bile acids became labeled to the same specific activity within 30 min after [3H]cholesterol was injected into bile fistula guinea pigs. When a mixture of [3H]ursodeoxycholic acid and [14C]chenodeoxycholic acid was infused into another bile fistula guinea pig, little 3H could be detected in either chenodeoxycholic acid or 7-ketolithocholic acid. But, 14C was efficiently incorporated into ursodeoxycholic and 7-ketolithocholic acids. Monohydroxylated bile acids make up 51% and ursodeoxycholic acid 38% of fecal bile acids. After 3 weeks of antibiotic therapy, lithocholic acid was reduced to 6% of the total, but ursodeoxycholic acid (5-11%) and 7-ketolithocholic (15-21%) acid persisted in bile. Lathosterol constituted 19% of skin sterols and was detected in the feces of an antibiotic-fed animal. After one bile fistula guinea pig suffered a partial biliary obstruction, ursodeoxycholic and 7-ketolithocholic acids increased to 46% and 22% of total bile acids, respectively. These results demonstrate that chenodeoxycholic acid, ursodeoxycholic acid, and 7-ketolithocholic acid can all be made in the liver of the guinea pig.
Tint
G S
GS
Department of Medicine, VA Medical Center, East Orange, NJ 07019.
Xu
G R
GR
Batta
A K
AK
Shefer
S
S
Niemann
W
W
Salen
G
G
D000818:Animals; D001647:Bile Acids and Salts / Q000032:analysis* / Q000378:metabolism; D002635:Chenodeoxycholic Acid / Q000032:analysis* / Q000378:metabolism; D002784:Cholesterol / Q000032:analysis; D003840:Deoxycholic Acid / Q000031:analogs & derivatives*; D005243:Feces / Q000032:analysis; D006168:Guinea Pigs; D007536:Isomerism; D007700:Kinetics; D008095:Lithocholic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D008297:Male; D014580:Ursodeoxycholic Acid / Q000032:analysis* / Q000378:metabolism
D001647:Bile Acids and Salts; D003840:Deoxycholic Acid; D002635:Chenodeoxycholic Acid; C023020:7-ketolithocholic acid; D008095:Lithocholic Acid; D014580:Ursodeoxycholic Acid; C001521:lathosterol; D002784:Cholesterol