Molecular hybridization studies with scrapie brain nucleic acids. II. Differential expression in scrapie hamster brain.
RNA and DNA fractions prepared from nucleic acids obtained from normal and scrapie-infected hamster brains following exhaustive treatment with nuclease were used as probes in a search for a specific scrapie nucleic acid. Employing RNA blot hybridization a 2.3 Kb RNA was detected in higher concentration in scrapie-infected hamster brains than in normal controls. Our data suggests that scrapie disease may be associated with the differential expression of a series of host genes.
Borrás
T
T
Merendino
J J
JJ
Gibbs
C J
CJ
D000818:Animals; D001921:Brain / Q000382:microbiology*; D006224:Cricetinae; D004247:DNA / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D008647:Mesocricetus; D009693:Nucleic Acid Hybridization; D011328:Prions / Q000235:genetics* / Q000472:pathogenicity; D012367:RNA, Viral / Q000032:analysis*; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid
D004279:DNA, Viral; D011328:Prions; D012367:RNA, Viral; D004247:DNA; D012194:RNA-Directed DNA Polymerase