Intermediate filaments of the midgestation rat trophoblast giant cell.
Trophectoderm (TE) of the rodent blastocyst, the preimplantation precursor of the trophoblast giant cell (TGC), is the first embryonic cell to exhibit intermediate filaments (IF). The two IF proteins of TE (54K and 46K) have been variously described as trophectoderm specific, noncytokeratin, or cytokeratin and have been identified with Endo A and Endo B, IF proteins extracted from extraembryonic endodermal cells. IF proteins of midgestation rat TGC, the postimplantation descendant of TE, were compared to IF proteins of various rat simple epithelial cells by two-dimensional gel electrophoresis, partial proteolytic digest, antibody recognition on electrophoretic transfer, and antibody recognition by indirect immunofluorescence. The two TE IF proteins at 54K and 46K were identified in TGC IF and recognized by anti-Endo A, anti-Endo B, respectively, and anticytokeratins. TGC were found to possess additional cytokeratins at 52K, 45K, 43K, and 40K. The profile of TGC cytokeratins was qualitatively identical to that of various rat simple epithelial cells. The results suggest that (a) TE and TGC IF proteins are cytokeratins, (b) TE and TGC cytokeratins are characteristic of a simple epithelial cell, and (c) the morphologic and functional differentiation of TE to TGC is accompanied by elaboration of the cytokeratin profile.
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Glasser
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J
Julian
D000818:Animals; D003599:Cytoskeleton / Q000648:ultrastructure*; D003893:Desmin / Q000032:analysis; D005455:Fluorescent Antibody Technique; D007381:Intermediate Filament Proteins / Q000032:analysis* / Q000276:immunology; D007382:Intermediate Filaments / Q000648:ultrastructure*; D007526:Isoelectric Point; D007633:Keratins / Q000032:analysis; D008099:Liver / Q000032:analysis; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D051381:Rats; D014018:Tissue Distribution; D014327:Trophoblasts / Q000032:analysis / Q000648:ultrastructure*; D014746:Vimentin / Q000032:analysis
D003893:Desmin; D007381:Intermediate Filament Proteins; D010446:Peptide Fragments; D014746:Vimentin; D007633:Keratins