Sialoglycoproteins with a high amount of O-glycosidically linked carbohydrate moieties stain yellow with silver in sodium dodecyl sulfate-polyacrylamide gels.
Several sialoglycoproteins and human salivary proteins were analyzed in sodium dodecyl sulfate-polyacrylamide gels using the silver/Coomassie-staining protocol (J. K. Dzandu, M. E. Deh, D. L. Barratt, and G. E. Wise, 1984, Proc. Natl. Acad. Sci. USA 81, 1733-1737) to determine the extent to which yellow Ag staining originally reported for human red blood cell glycophorins can be applied to other sialoglycoproteins. Results showed that not all sialoglycoproteins elicit a positive yellow color in the silver stain reaction. Some of the sialoglycoproteins stained as brown or negative images in the Ag-staining cycle. Alkaline beta elimination of O-glycosidically linked carbohydrate chains of glycophorin resulted in the loss of yellow color development in the Ag-staining protocol. Analysis of acidic salivary proteins showed several yellow Ag-stained bands at Mr X 10(-3) = 150, 82, 70, 51, 46, and 42. These results suggest that the carbohydrate moieties of glycophorin removable by alkaline beta elimination are responsible for the characteristic yellow color in the Ag stain reaction. In addition, under our staining conditions sialoglycoproteins with a high amount of O-glycosidically linked carbohydrate chains give a characteristic yellow silver stain.
M E
ME
Deh
J K
JK
Dzandu
G E
GE
Wise
D002241:Carbohydrates / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D003116:Color; D004591:Electrophoresis, Polyacrylamide Gel; D006027:Glycosides / Q000032:analysis; D006801:Humans; D012471:Salivary Proteins and Peptides / Q000032:analysis; D012795:Sialoglycoproteins / Q000032:analysis*; D012834:Silver; D013194:Staining and Labeling
D002241:Carbohydrates; D006027:Glycosides; D012471:Salivary Proteins and Peptides; D012795:Sialoglycoproteins; D012834:Silver