Selection of a rat glutamine synthetase cDNA clone.
We have selected a glutamine synthetase clone (pGSRK-1) from a rat kidney cDNA library. A partial restriction map has been constructed for the 1.65 kilobase pair (kbp) glutamine synthetase cDNA. Northern hybridization analysis indicates that 1) GS-specific RNA increases many-fold during adipocyte differentiation and 2) dexamethasone increases and insulin decreases GS-specific RNA in 3T3-L1 adipocytes.
D M
DM
Burns
B
B
Bhandari
J M
JM
Short
P G
PG
Sanders
R H
RH
Wilson
R E
RE
Miller
D000273:Adipose Tissue; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology; D005974:Glutamate-Ammonia Ligase / Q000235:genetics*; D007328:Insulin / Q000494:pharmacology; D007668:Kidney / Q000201:enzymology; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000378:metabolism; D051381:Rats
D007328:Insulin; D012313:RNA; D003907:Dexamethasone; D004247:DNA; D004262:DNA Restriction Enzymes; D005974:Glutamate-Ammonia Ligase