Salivary histatin 5: dependence of sequence, chain length, and helical conformation for candidacidal activity.
Histatin 5 (Asp1-Ser-His-Ala4-Lys-Arg-His-His8-Gly-Tyr-Lys-Arg12-Lys-Ph e-His-Glu16-Lys-His - His-Ser20-His-Arg-Gly-Tyr24), one of the basic histidine-rich peptides present in human parotid saliva and several of its fragments, 1-16 (N16), 9-24 (C16), 11-24 (C14), 13-24 (C12), 15-24 (C10), and 7-16 (M10), were synthesized by solid-phase procedures. Native histatin 5 from human parotid saliva was also purified. Their antifungal activities on two strains of Candida albicans have been studied and their conformational preferences both in aqueous and non-aqueous solutions examined by circular dichroism. The synthetic histatin 5, C16, and C14 peptides were highly active and inhibited the growth of C. albicans. The candidacidal activity data of synthetic histatin 5 were comparable to the values of the native histatin 5 isolated from parotid saliva and those reported previously, although the assay system used and the strains examined were different. The C16 fragment was as active as the whole peptide itself, whereas the N16 fragment was far less active than C14, suggesting that the sequence at the C-terminal is important for its fungicidal activity. An increase in the chain length of the C-terminal sequence from 12 to 16 residues increased the candidacidal activity, thereby indicating that a peptide chain length of at least 12 residues is necessary to elicit optimum biological activity. The CD spectra of these linear peptides showed that they are structurally more flexible, and they adopt different conformations depending on the solvent environment. CD studies provided evidence that histatin 5 and the longer fragments, C16, N16, and C14 preferred alpha-helical conformations in non-aqueous solvents such as trifluoroethanol and methanol, while in water and pH 7.4 phosphate buffers, they favored random coil structures. The shorter sequences seemed to adopt either turn structures or unordered structures both in aqueous and non-aqueous solutions. It appears that the sequence at the C-terminal of histatin 5 with a minimum chain length of 14 residues and alpha-helical conformation are the important structural requirements for appreciable candidacidal activity.
Department of Oral Biology, School of Dental Medicine, State University of New York, Buffalo 14214.
P A
PA
Raj
M
M
Edgerton
M J
MJ
Levine
D000595:Amino Acid Sequence; D000935:Antifungal Agents; D002176:Candida albicans / Q000187:drug effects; D002942:Circular Dichroism; D054808:Histatins; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010306:Parotid Gland / Q000378:metabolism; D010446:Peptide Fragments; D010455:Peptides / Q000138:chemical synthesis; D011487:Protein Conformation; D012463:Saliva / Q000378:metabolism*; D012471:Salivary Proteins and Peptides / Q000235:genetics* / Q000494:pharmacology; D013329:Structure-Activity Relationship
D000935:Antifungal Agents; C068075:HTN3 protein, human; D054808:Histatins; D010446:Peptide Fragments; D010455:Peptides; D012471:Salivary Proteins and Peptides