Iron deficiency in growing male rats: a cause of development of cardiomyopathy.
Weanling male rats were fed a purified iron-adequate, a purified iron-deficient or a commercial diet for 12 weeks. At that time the rats were sacrificed, their hearts and livers were excised, and blood samples were taken. Heart and liver weights were recorded; organ tissue and serum samples were analyzed for Zn, Cu and Fe. Hemoglobin and hematocrit values were also obtained. The iron-deficient rats grew much more slowly than controls on the iron-adequate or commercial diets. The iron-deficient rats were severely anemic and had enlarged hearts (cardiomegaly). A histopathologic examination of the hearts of all animals showed that each heart of the iron-deficient rats had lesions characteristic of cardiomyopathy by dilatation, whereas none of the hearts of the iron-adequate group or the chow controls showed any lesions at all. The iron-deficient animals had only about 25% of the hepatic iron found in the iron-adequate animals but about 5 times the hepatic copper of the latter group or the chow controls. Heart iron of the iron-deficient group was 27% of the concentration found in hearts of the iron-adequate rats; heart copper was similar in all groups. Animals on the commercial stock diet accumulated significantly more iron in their hearts than did those on the purified iron-adequate diet but not in the livers. There was also a direct correlation of heart iron or heart zinc with log concentrations of dietary iron and consequently a direct correlation between heart iron and zinc concentrations.
Department of Chemistry, University of Wisconsin-Milwaukee.
D H
DH
Petering
K L
KL
Stemmer
S
S
Lyman
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S
Krezoski
H G
HG
Petering
D000818:Animals; D009202:Cardiomyopathies / Q000209:etiology* / Q000473:pathology; D003300:Copper / Q000097:blood; D004032:Diet; D007501:Iron / Q000097:blood; D000090463:Iron Deficiencies; D008099:Liver / Q000032:analysis; D008297:Male; D009206:Myocardium / Q000032:analysis / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D013054:Spectrophotometry, Atomic; D015032:Zinc / Q000097:blood
D003300:Copper; D007501:Iron; D015032:Zinc