Use of commercially available rapid chloramphenicol acetyltransferase test to detect resistance in Salmonella species.
Chloramphenicol resistance among Salmonella spp. has important public health and clinical implications, especially in areas of the world where these strains are endemic. The availability of rapid and sensitive screening methods for detection of antibiotic resistance is important. Therefore, we tested 33 strains of Salmonella for chloramphenicol acetyltransferase (CAT) activity using two rapid techniques. Evaluation of a 1-h tube method and a 30-min commercial disk procedure demonstrated that they are as accurate as standardized susceptibility techniques. Both the 1-h tube and 30-min disk methods detected CAT enzymatic activity produced by one CAT gene copy per cell.
Francis Blake Bacteriology Laboratories, Massachusetts General Hospital, Boston 02114.
L
L
de la Maza
S I
SI
Miller
M J
MJ
Ferraro
D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics / Q000378:metabolism*; D002702:Chloramphenicol Resistance / Q000235:genetics; D006801:Humans; D008826:Microbial Sensitivity Tests; D012475:Salmonella / Q000201:enzymology* / Q000235:genetics
D015500:Chloramphenicol O-Acetyltransferase