Detection of herpes simplex virus proteins in cultured cells by monoclonal antibodies and the avidin-biotin-immunoperoxidase complex method.
The distribution of 5 proteins of herpes simplex virus Type 1 was observed in cells that had been infected for various periods. The cells were stained with monoclonal antibodies to ICP4, ICP5, ICP6, ICP8, and gB, using the avidin-biotin-peroxidase complex (ABC) method. Each protein had a characteristic pattern of time of appearance and translocation by which it could be distinguished from the others. The sensitivity of the ABC technique, its ease of use, and the permanence of the preparations make this method well suited for the study of viral proteins.
C B
CB
Hwang
J S
JS
Greenspan
E J
EJ
Shillitoe
D000818:Animals; D000911:Antibodies, Monoclonal; D001360:Avidin; D001710:Biotin; D002460:Cell Line; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D006801:Humans; D007124:Immunoenzyme Techniques; D007668:Kidney; D018139:Simplexvirus / Q000276:immunology*; D013194:Staining and Labeling; D013997:Time Factors; D014764:Viral Proteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D014764:Viral Proteins; D001360:Avidin; D001710:Biotin