Disparate gene expression of chemotactic cytokines by human mononuclear phagocytes.
Chemotactic cytokines are becoming increasingly recognized as important participants in the coordinate recruitment of specific inflammatory cells. In this manuscript we present data demonstrating that LPS challenged human mononuclear phagocytic cells can express mRNA for neutrophil chemotactic factor/interleukin-8 (NCF/IL-8), but do not express mRNA for monocyte chemotactic protein (MCP). The expression of NCF/IL-8 mRNA was time and dose dependent. This identical stimulus response was also found in peripheral blood neutrophils. These studies demonstrate a disparate production of chemotactic cytokines by macrophages and exemplify the dynamic nature of the chemotactic response.
Department of Internal Medicine, University of Michigan Medical School, Ann Arbor.
R M
RM
Strieter
S W
SW
Chensue
T J
TJ
Standiford
M A
MA
Basha
H J
HJ
Showell
S L
SL
Kunkel
D001483:Base Sequence; D015152:Blotting, Northern; D018932:Chemokine CCL2; D002630:Chemotactic Factors / Q000235:genetics*; D006801:Humans; D016209:Interleukin-8; D007378:Interleukins / Q000235:genetics*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008214:Lymphocytes / Q000502:physiology; D008264:Macrophages / Q000502:physiology*; D008969:Molecular Sequence Data; D009000:Monocytes / Q000502:physiology*; D009504:Neutrophils / Q000502:physiology; D015345:Oligonucleotide Probes; D011650:Pulmonary Alveoli / Q000166:cytology; D012333:RNA, Messenger / Q000235:genetics
D018932:Chemokine CCL2; D002630:Chemotactic Factors; D016209:Interleukin-8; D007378:Interleukins; D008070:Lipopolysaccharides; D015345:Oligonucleotide Probes; D012333:RNA, Messenger