Interaction of the pyruvate dehydrogenase complex from human heart with thiamine pyrophosphate.
Preparations of a highly purified pyruvate dehydrogenase complex (PDC) from human heart contain endogenous thiamine pyrophosphate (TPP) in an amount accounting for about 10% of the maximum activity. At pH values of 7.5 and 8.0, the effective Michaelis constants with respect to exogenous TPP for the PDC apoenzyme form were 0.22 microM and 1.8 microM, respectively.
Institute of Biochemistry, Byelorussian SSR Academy of Sciences, Grodno, USSR.
Kiselevsky YuV
S A
SA
Ostrovtsova
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Strumilo
D004789:Enzyme Activation; D006801:Humans; D007700:Kinetics; D008929:Mitochondria, Heart / Q000201:enzymology*; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism*; D013835:Thiamine Pyrophosphate / Q000378:metabolism*
D011768:Pyruvate Dehydrogenase Complex; D013835:Thiamine Pyrophosphate