Molecular biology for nonmolecular biologists.
Molecular biology offers various powerful tools for understanding the etiology of diabetes mellitus. These methods can be understood by nonmolecular biologists. Topics briefly introduced include 1) control of gene expression, 2) cloning of genes, 3) how gene promoters work, 4) transgenic mice, 5) mutagenesis in vitro, 6) human molecular genetics, and 7) the new powerful polymerase chain-reaction technique, which greatly simplifies many of the other methods.
Chirgwin
J M
JM
Department of Medicine, University of Texas Health Science Center, San Antonio 78284.
D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D003920:Diabetes Mellitus / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D008967:Molecular Biology / Q000379:methods; D009154:Mutation; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic
D004247:DNA