[Effects of triptolide on T lymphocyte functions in mice].
Triptolide (Tri) is an active principle of Tripterygium hypoglaucum Hutch, which has been used to treat cancers and autoimmune diseases in folk medicine. The lymphocyte proliferation (LP) induced by Con A or Lipopolysaccharide (LPS) was suppressed by Tri at 50 and 500 ng/ml. At 5 ng/ml the suppressive effect on LP induced by Con A was much stronger than that by LPS. The humoral immune reaction monitored by quantitative hemolysis spectrophotometry (QHS) was suppressed by Tri 0.75 and 1 mg/kg ip. IL-2 production by mouse spleen cells were suppressed by Tri 5, 50,500 ng/ml in vitro and 0.5, 0.75 and 1 mg/kg ip. IL-2 activity was assayed by assessing its ability to support proliferation of selected IL-2-dependent T cell line, measured by colorimetric method which is based on the ability of viable cells to cleave MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide). Ts cells induced by supraoptimal immune (SOI) were suppressed by Tri 0.5 and 0.75 mg/kg ip. These results suggest T or its subpopulations and B lymphocytes may be the target cells of Tri.
Pu
L X
LX
He-nan Institute of Medical Sciences, Zhengzhou, China.
Zhang
T M
TM
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004224:Diterpenes / Q000494:pharmacology*; D004852:Epoxy Compounds; D005260:Female; D006461:Hemolysis; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D010616:Phenanthrenes; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
D004224:Diterpenes; D004852:Epoxy Compounds; D007376:Interleukin-2; D010616:Phenanthrenes; C001899:triptolide