Isolation and characterization of Ni-specific T cell clones from patients with Ni-contact dermatitis.
Ni-specific T lymphocyte clones (TLC) were isolated from two patients with Ni-contact dermatitis. All of the isolated TLC required both histocompatible antigen-presenting cells (APC) and Ni for induction of proliferation. By using a panel of HLA-typed Epstein Barr virus-transformed B cells (EBV-B cells) as APC and monoclonal anti-DR antibody, the clones were shown to recognize Ni in the context of HLA class II determinants. All of the clones that were isolated are OKT3+, OKT4+, OKT8-. In the presence of Ni, they polyclonally activate autologous B cells, and in the presence of Ni and autologous EBV-B cells, they produce IL 2 and very high levels of IFN-gamma. The Ni-specific clones should be helpful in the identification of the Ni-induced antigen which is recognized by T cells.
F
F
Sinigaglia
D
D
Scheidegger
G
G
Garotta
R
R
Scheper
M
M
Pletscher
A
A
Lanzavecchia
D000938:Antigen-Presenting Cells / Q000145:classification; D002469:Cell Separation / Q000379:methods*; D002999:Clone Cells / Q000145:classification / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D003877:Dermatitis, Contact / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D005260:Female; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D008222:Lymphokines / Q000096:biosynthesis; D009532:Nickel / Q000276:immunology*; D010641:Phenotype; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
D000939:Epitopes; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D008222:Lymphokines; D009532:Nickel