[Suitability of specific staining methods for the estimation of beta cell volume in rat pancreas with normal and reduced insulin content].
Investigations were carried out on streptozotocin (SZ)-treated female Wistar rats (single i.v. injection of 30 mg/kg body weight) and control animals with normal pancreatic insulin content. After staining of the pancreatic slices with aldehyde fuchsin (AF), Victoria Blue (Ivic 1959), and FITC-labelled antiserum (indirect), the beta-cell volume was determined with a point sampling method. For control animals, all 3 selected beta-cell specific staining methods are equivalent. After reduction of the pancreatic and the beta-cell insulin content by SZ-treatment the sensitivity of the common histochemical methods is diminished in comparison to the immunofluorescence method. In normoglycemia SZ-treated animals (pancreatic insulin content about 30% of control values) 0.22% AF-stained beta-cells are demonstrable, after IVIC-staining 0.14% beta-cell volume is visible. In hyperglycemic SZ-treated rats (about 6% of pancreatic insulin content of controls) with AF-staining only 0.08% and with IVIC-staining 0.03% beta-cell volume could be measured whereas with fluorescence technique in both SZ treated groups 0.34% beta-cell volume is measurable. The reduction of pancreatic insulin content after the injection of 30 mg/kg SZ is caused by a reduction of the beta-cell volume and a diminution of the insulin content in the remaining beta-cells.
V S
VS
Lucke
B
B
Ziegler
J M
JM
Diaz-Alonso
H J
HJ
Hahn
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000473:pathology; D005260:Female; D005455:Fluorescent Antibody Technique; D007328:Insulin / Q000378:metabolism*; D007515:Islets of Langerhans / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling
D007328:Insulin
Eignung spezifischer Färbemethoden für die Bestimmung des beta-Zellvolumens im Rattenpankreas mit normalem und reduziertem Insulingehalt.