The use of antibody immobilized on supports coated with polyglycidyl methacrylate in saturation analysis.
A new procedure for separation of free and bound ligand in saturation analysis (e.g., radioimmunoassay, competitive protein binding analysis) is presented. The antibody was immobilized on different carriers (glass rods, aluminium or polyethylene strips) covered with a thin layer of polyglycidyl methacrylate. The surface of the polymer had been activated by reaction with either ethylene diamine and glutaraldehyde or sulfuric acid and sodium periodate. The antibody was immobilized on this activated polymer by a covalent bond. The advantages of the presented separation methods are rapidity, simplicity, and conservation of the free and bound ligand equilibrium. A comparison with other separation techniques is carried out.
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Rauch
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Marek
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Matas
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Vodrázka
D000180:Acrylic Resins; D000906:Antibodies; D001798:Blood Proteins; D003488:Cyanogen Bromide; D003911:Dextrans; D007158:Immunologic Techniques; D008024:Ligands; D010206:Papain; D011109:Polymethacrylic Acids; D011485:Protein Binding; D011863:Radioimmunoassay
D000180:Acrylic Resins; D000906:Antibodies; D001798:Blood Proteins; D003911:Dextrans; D008024:Ligands; D011109:Polymethacrylic Acids; C016680:polyacrylamide gels; C042535:polyglycidyl methacrylate; C025614:sephadex; D010206:Papain; D003488:Cyanogen Bromide