Kidney and adrenal mitochondria contain two forms of NADPH-adrenodoxin reductase-dependent iron-sulfur proteins. Isolation of the two porcine renal ferredoxins.
Two NADPH-adrenodoxin reductase-dependent iron-sulfur proteins were detected in both porcine kidney and bovine adrenal mitochondria by using high resolution polyacrylamide electrophoresis. Adrenodoxin (Mr = 12,000) constituted the major ferredoxin activity in adrenal mitochondria and a similarly sized protein (Mr = 11,500) was isolated as the major renal ferredoxin activity. A second, higher molecular weight ferredoxin was observed in both adrenal (Mr = 13,300) and kidney (Mr = 13,000) mitochondria. The two renal ferredoxins were isolated by the use of ion exchange, gel exclusion, and preparative electrophoretic techniques. An absorption spectrum typical of [2Fe-2S] ferredoxins was obtained for each protein; however, the larger renal molecule had an unusually high 276 nm absorbance. Immunologic studies revealed a significant degree of antigenic commonality between the two renal proteins as well as specific cross-reactivity of adrenodoxin with antiserum raised against the renal proteins. A possible precursor-product relationship between the paired renal and adrenal ferredoxins is discussed.
W J
WJ
Driscoll
J L
JL
Omdahl
D000311:Adrenal Glands / Q000648:ultrastructure*; D000325:Adrenodoxin / Q000032:analysis; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D005287:Ferredoxin-NADP Reductase / Q000378:metabolism*; D005288:Ferredoxins / Q000302:isolation & purification*; D007506:Iron-Sulfur Proteins / Q000302:isolation & purification*; D007668:Kidney / Q000648:ultrastructure*; D008667:Metalloproteins / Q000302:isolation & purification*; D008928:Mitochondria / Q000032:analysis*; D008970:Molecular Weight; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D013552:Swine
D000939:Epitopes; D005288:Ferredoxins; D007506:Iron-Sulfur Proteins; D008667:Metalloproteins; D000325:Adrenodoxin; D005287:Ferredoxin-NADP Reductase; D009247:NADH, NADPH Oxidoreductases