The production of peste des petits ruminants hyperimmune sera in rabbits and their application in virus diagnosis.
Hyperimmune sera were produced by serial inoculation of rabbits with Vero cell-adapted, sucrose gradient-purified Nigerian peste des petits ruminants virus (PPRV) isolate. Two antisera produced, neutralized the homologous PPRV but not the heterologous rinderpest Kabette "O" virus. The antisera gave strong precipitin lines with purified PPRV antigens and were used to detect PPRV and rinderpest virus antigens from ante-mortem secretions and post-mortem tissue homogenates from PPR and rinderpest virus infected goats and cattle by the agar gel precipitation tests (AGPT). The hyperimmune sera gave good titration curves with both purified Nigerian goat and the United Arab Emirate wildlife PPRV isolates in the indirect enzyme linked immunosorbent assay (ELISA). Results of indirect ELISA showed that although there were some cross reactions with the rinderpest, canine-distemper and measles viruses, at 1:100 dilution, the antisera would give a positive signal with only the homologous PPR virus.
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Institute for Animal Disease Research, Pirbright Laboratory, Woking Surrey, U.K.
McCullough
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Taylor
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WP
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D007106:Immune Sera / Q000276:immunology; D011817:Rabbits; D012301:Rinderpest / Q000175:diagnosis*; D012302:Rinderpest virus / Q000276:immunology*; D014709:Vero Cells
D000914:Antibodies, Viral; D007106:Immune Sera