[Method for isolating single cardiac cells in the guinea pig using pronase. The role of calcium ions].
A method for isolation of guinea-pig cardiomyocytes with pronase has been developed. The method has been assessed in hearts perfused with solutions containing pronase (1 U/ml) and 200 microM Ca2+. Eighty per cent of the cells released were rod-shaped and 1.2 mM Ca2+ tolerant. Enriched medium 199 was used for all solutions. Sodium and slow inward currents recorded from cells dispersed with pronase were similar to those recorded from cells isolated after prolonged exposure to collagenase. Two principal factors are to be marked: (a) presence of high enough amounts of Ca2+ in enzyme solution (up to 200 microM); (b) use of the enriched medium in all the stages of the procedure.
Bendukidze
Z A
ZA
Lopatin
A N
AN
Ignat'eva
V B
VB
Sokolova
S N
SN
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002469:Cell Separation; D003584:Cytological Techniques; D006168:Guinea Pigs; D006321:Heart / Q000187:drug effects* / Q000502:physiology; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism; D011402:Pronase / Q000494:pharmacology*; D012964:Sodium / Q000378:metabolism
D007473:Ion Channels; D012964:Sodium; D011402:Pronase; D002118:Calcium
Metod vydeleniia odinochnyklh serdechnykh kletok morskoiĭ svinki s pomoshch'iu pronazy. Rol' ionov kal'tsiia.