Localization of lysozyme in the frozen section of nasal mucosa by protein A-gold technique.
The location of lysozyme was studied in frozen sections of the nasal gland by protein A-gold technique, modified for the frozen section. Serous glands were enriched with lysozyme. Within the glands, the apical portion of the serous cells was most heavily stained. The epithelial layer was virtually unstained for lysozyme. The result was essentially the same as that obtained by the peroxidase-antiperoxidase method. Electronmicroscopic examination of the stained sections supports the possible application of this method for pre-embedding immunostaining, although further improvement of the technique is necessary.
M
M
Tachibana
H
H
Morioka
T
T
Tsuruoka
M
M
Machino
O
O
Mizukoshi
D003594:Cytoplasmic Granules / Q000201:enzymology; D006046:Gold; D006801:Humans; D008854:Microscopy, Electron; D009113:Muramidase / Q000378:metabolism*; D009297:Nasal Mucosa / Q000201:enzymology*; D013194:Staining and Labeling; D013205:Staphylococcal Protein A
D013205:Staphylococcal Protein A; D006046:Gold; D009113:Muramidase