Microscopic and flow cytophotometric analysis of parasitemia in cultures of Plasmodium falciparum vitally stained with Hoechst 33342--application to studies of antimalarial agents.
Conditions for rapid vital staining of Plasmodium falciparum infected human erythrocytes were 1 microgram/ml of the dye Hoechst 33342 for 15 min in the standard culture medium at 37 degrees C. Fixed and stained cultures were analyzed by fluorescence microscopy and flow cytophotometry. The usefulness of this type of analysis for in vitro studies of antimalarial agents was demonstrated using three such agents--cyclosporin A, chloroquine, and pyrimethamine.
Franklin
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RM
Brun
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Grieder
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A
D000962:Antimalarials / Q000494:pharmacology*; D001562:Benzimidazoles; D002738:Chloroquine / Q000494:pharmacology; D003524:Cyclosporins / Q000494:pharmacology; D004247:DNA / Q000032:analysis; D004912:Erythrocytes / Q000469:parasitology*; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006801:Humans; D008288:Malaria / Q000188:drug therapy / Q000469:parasitology*; D008856:Microscopy, Fluorescence; D010963:Plasmodium falciparum / Q000032:analysis / Q000187:drug effects* / Q000254:growth & development; D011739:Pyrimethamine / Q000494:pharmacology; D013194:Staining and Labeling
D000962:Antimalarials; D001562:Benzimidazoles; D003524:Cyclosporins; D005456:Fluorescent Dyes; D002738:Chloroquine; D004247:DNA; C017807:bisbenzimide ethoxide trihydrochloride; D011739:Pyrimethamine