Metabolic control analysis. The effects of high enzyme concentrations.
Differing views have been given in the literature as to whether the presence in a pathway of an enzyme at a concentration comparable to that of its substrate affects the values of control coefficients and the theorems of metabolic control analysis. Here we argue in favour of one of those views: that there is no effect unless the enzyme sequesters a substrate that contains a conserved moiety. In this particular case, we derive both a general criterion for estimating whether such an effect will be of a significant magnitude, and equations for determining the changes in the flux control coefficients. The nature of the phenomenom and the application of the equations are illustrated with a numerical simulation.
School of Biological and Molecular Sciences, Oxford Polytechnic, England.
D A
DA
Fell
H M
HM
Sauro
D004798:Enzymes / Q000378:metabolism*; D007700:Kinetics; D008432:Mathematical Computing; D008954:Models, Biological
D004798:Enzymes