[Expression in Chinese hamster transformant TK+-cells of the bacterial gene for beta-lactamase].
Using monospecific immune antiserum the expression of beta-lactamase bacterial gene (from the plasmid pBR322) in transformed TK+ Chinese hamster cells was studied. It is shown that in some TK+-clones the beta-lactamase gene is expressed and this expression is stimulated by an inhibitor of DNA methylation-5-azacytidine. Two physically linked genes (the Herpes simplex thymidine kinase gene, and the beta-lactamase gene) introduced into the Chinese hamster cells on the same plasmid are found to be expressed independently.
Tomilin
N V
NV
Glebov
O K
OK
Mikhaĭlov
V M
VM
Sasina
L K
LK
Abramian
D S
DS
D000818:Animals; D001374:Azacitidine / Q000494:pharmacology; D002999:Clone Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D006224:Cricetinae; D003412:Cricetulus; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D005798:Genes, Bacterial / Q000187:drug effects; D010641:Phenotype; D010957:Plasmids / Q000187:drug effects; D018139:Simplexvirus / Q000235:genetics; D013268:Stimulation, Chemical; D013937:Thymidine Kinase / Q000235:genetics*; D014170:Transformation, Genetic / Q000187:drug effects; D001618:beta-Lactamases / Q000235:genetics*
D013937:Thymidine Kinase; D001618:beta-Lactamases; D001374:Azacitidine
Ekspressiia v transformantnykh TK-kletkakh kitaĭskogo khomiachka bakterial'nogo gena beta-laktamazy.