Reliable preparative procedures for the cytological study of yolk-laden insect eggs and embryos.
Yolk-laden insect eggs and embryos are easy to fix and section by the following procedure: Fix with non- or slightly coagulant solutions after opening the envelopes for large eggs, or after superficial fixation and removal of the vitelline membrane for small ones. Carefully embed fixed and washed specimens in a thick agar gel. Dehydrate trimmed agar blocks, first with 70% ethanol, then with 2-ethoxy-ethanol. After optional immersion in butanol-1, embed in Steedman's "Ester wax 1960." Section, mount and stain like paraffin sections. Results are compared to the cryosectioning method recently described by Hartmann.
Pétavy
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D000362:Agar; D000818:Animals; D004331:Drosophila melanogaster; D004952:Esters; D005404:Fixatives; D006110:Grasshoppers; D006651:Histocytochemistry; D008867:Microtomy; D010063:Ovum / Q000032:analysis / Q000166:cytology*; D013194:Staining and Labeling / Q000379:methods; D014885:Waxes; D015017:Yolk Sac / Q000032:analysis / Q000166:cytology*
D004952:Esters; D005404:Fixatives; D014885:Waxes; D000362:Agar