[Kinetic properties of 5'-nucleotidase in the adipose tissue, liver and blood of rats of different ages].
The kinetic characteristics (Vmax and Km) of membrane-bound and serum 5'-nucleotidases from rats of different age groups are studied. The age-related increase of Km parameter in plasma membranes of fatty tissue and liver demonstrated a lower enzyme affinity to the substrate in ageing membranes. The specific activity of 5'-nucleotidase in blood serum of aged animals was below that in young ones, while Km value remained stable. Tissue differentiation of the catalytic properties of the enzyme and their aged-related characteristics may be to a considerable extent determined by the physicochemical state of cell membranes.
Egutkin
G G
GG
Iakubovskiĭ
S M
SM
Gatsko
G G
GG
D015720:5'-Nucleotidase / Q000097:blood / Q000378:metabolism*; D000273:Adipose Tissue / Q000201:enzymology*; D000375:Aging / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D015720:5'-Nucleotidase
Kineticheskie svoĭstva 5'-nukleotidazy v zhirovoĭ tkani, pecheni i krovi krys raznogo vozrasta.