Effects of flow on the synthesis and release of fibronectin by endothelial cells.
Human umbilical vein endothelial cells at confluence were subjected to steady shear flow. The effect of flow on the synthesis of fibronectin, its release into the medium, and incorporation into the extracellular matrix were investigated. The total content of fibronectin in endothelial cells exposed to flow was found to be lower than that in static controls after periods of 12 to 48 h. In the presence of cycloheximide there was no difference in the fibronectin content of sheared and unsheared cells. Our results suggest that the synthesis of fibronectin is inhibited by the flow-induced perturbation of endothelial cells.
Gupte
A
A
Department of Chemical Engineering, Pennsylvania State University, University Park 16802.
Frangos
J A
JA
D003513:Cycloheximide / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D012212:Rheology; D013314:Stress, Mechanical; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013917:Thrombin / Q000494:pharmacology; D014471:Umbilical Veins
D005353:Fibronectins; D003513:Cycloheximide; D013917:Thrombin; D013755:Tetradecanoylphorbol Acetate