Manifestation of allo H-2-restriction specificity by self H-2-restricted T cells.
Previous study has demonstrated that a considerable number of antigen-specific, allo H-2-restricted CTLPs could be recovered from normal lymphocytes. Experiments were conducted to examine whether antigen-specific, self H-2-restricted T cells could also manifest allo H-2-restriction specificity. Non-H-2-specific, self H-2-restricted CTLs were induced in the microcultures with a limited number of responder spleen cells from B10(H-2b) mice that had been primed with C3H.SW(H-2b) spleen cells. Two hundred thirteen non-H-2-specific CTLs generated in limiting dilution culture condition were assayed for cross-reactivity against TNP-modified and unmodified allogeneic targets of 10 different H-2 haplotypes. Of these CTLs, 29 (14%) were cross-reactive to a TNP-modified allogeneic target and seventeen (8%) to an unmodified allogeneic target. The quantitative analysis for cross-reactivity of non-H-2 specific, self H-2-restricted CTLs reveals a significant number of CTLs that were cross-reactive with either alloantigen or antigen (TNP) plus allo H-2. Furthermore, CTL clones with triple specificities including self H-2-restriction specificity, allo-reactivity, and allo H-2 restriction specificity were also found. These results indicate that antigen-specific, allo H-2-restricted T cells are distinct not only from alloreactive T cells but also from antigen-specific, self H-2-restricted T cells. Significance of the manifestation of allo H-2 restriction specificity by self H-2-restricted T cells is discussed in regard to the generation of T cell repertoire.
Aizawa
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S
Division of Physiology and Pathology, National Institute of Radiological Sciences, Chiba, Japan.
Sado
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T
D000818:Animals; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic; D006183:H-2 Antigens / Q000276:immunology*; D006239:Haplotypes; D007111:Immunity, Cellular; D051379:Mice; D008815:Mice, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014301:Trinitrobenzenes / Q000276:immunology
D006183:H-2 Antigens; D014301:Trinitrobenzenes