Demonstration of pregnancy-associated plasma protein-A (PAPP-A)-like material in the fallopian tube.
The distribution and concentration of pregnancy-associated plasma protein-A (PAPP-A) in the human fallopian tube were examined by the immunoperoxidase staining technique and radioimmunoassay as part of a detailed study of PAPP-A in the nonpregnant state. PAPP-A-like material was identified in the epithelial cells of the mucosa in all fallopian tube specimens examined (n = 21). The intensity of the staining for PAPP-A was unrelated to the phase of the menstrual cycle. PAPP-A-like material was detected in saline extracts from all tubal tissues (n = 14) but not in any of the sera obtained from the same patients. The tissue concentration (mean +/- standard error of the mean) of immunoreactive PAPP-A varied from 15.2 +/- 1.1 to 30.1 +/- 4.2 micrograms/g protein in different parts of the tubes. No difference in the PAPP-A concentration was found between isthmic, ampullar, and fimbrial part of the tube, but proliferative phase tube seems to contain more PAPP-A than secretory phase tube. The PAPP-A measured in the fallopian tube appears to be similar in molecular size and antigenicity to that of pregnancy.
J
J
Sjöberg
T
T
Wahlström
J G
JG
Grudzinskas
M J
MJ
Sinosich
D000328:Adult; D002850:Chromatography, Gel; D004848:Epithelium / Q000032:analysis; D005187:Fallopian Tubes / Q000032:analysis* / Q000601:surgery; D005260:Female; D005831:Genital Diseases, Female / Q000601:surgery; D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D007889:Leiomyoma / Q000601:surgery; D008597:Menstrual Cycle; D008875:Middle Aged; D009092:Mucous Membrane / Q000032:analysis; D011257:Pregnancy Proteins / Q000032:analysis*; D011266:Pregnancy-Associated Plasma Protein-A / Q000032:analysis*; D011863:Radioimmunoassay; D014594:Uterine Neoplasms / Q000601:surgery
D011257:Pregnancy Proteins; D011266:Pregnancy-Associated Plasma Protein-A