Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion.
Hybridomas producing mouse monoclonal antibodies to antigens of the human mammary carcinoma cell line, MCF-7, have been generated by electric field-mediated fusion at a frequency ten times higher than by polyethylene glycol. One of the monoclonal antibodies obtained recognizes a cytoskeletal structure restricted to epithelial cells and carcinomas with a distribution pattern resembling cytokeratin 19.
Karsten
U
U
Papsdorf
G
G
Roloff
G
G
Stolley
P
P
Abel
H
H
Walther
I
I
Weiss
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000951:Antigens, Neoplasm / Q000276:immunology; D002459:Cell Fusion; D002460:Cell Line; D004560:Electricity; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007633:Keratins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010954:Plasmacytoma / Q000276:immunology; D011092:Polyethylene Glycols; D013154:Spleen / Q000276:immunology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D011092:Polyethylene Glycols; D007633:Keratins