Immunological characterization of microtubule-associated proteins specific for the immature brain.
Immunoblotting analysis was used to detect the microtubule-associated proteins present at different stages of rat brain development. Polyclonal antibodies were raised against the two main adult brain microtubule-associated proteins: MAP-2 (300 kDa) and TAU (60-70 kDa). Whatever the stage of development, anti-MAP-2 serum detected high molecular mass proteins and at immature stages a protein of 62 kDa. This protein which has previously been referred to as 'young TAU slow' is, therefore, immunologically related to MAP-2. The anti-TAU serum (but not the anti-MAP-2 serum) detected at immature stages of development a 48 kDa protein which also disappears at adulthood. This 48 kDa entity which has been referred to as 'young TAU fast' is progressively replaced by the closely spaced bands (60-70 kDa) of adult TAU proteins. The 62 and 48 kDa proteins appear therefore to be immunologically distinct and represent two microtubule-associated proteins specific to the immature brain.
Couchie
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Nunez
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D000818:Animals; D001921:Brain / Q000254:growth & development; D001923:Brain Chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D007120:Immunochemistry; D008869:Microtubule-Associated Proteins / Q000032:analysis*; D051381:Rats; D013194:Staining and Labeling
D008869:Microtubule-Associated Proteins