Proliferative characteristics of human haemopoietic cells in culture.
The proliferative characteristics of haemopoietic cells, either stimulated by PHA or cultured in the mixed colony assay, the CFU-GM assay or in the leukaemic blast cell assay, was studied using BrdU incorporation and sister chromatid differential staining. There were considerable differences between the various cell types in their proliferative characteristics. Cells cultured in the mixed colony assay showed the greatest proliferative activity with 18% of cells completing two cell cycles in 18 h. PHA stimulated lymphocytes and cells cultured in the leukaemic blast cell assay proliferated at a slower rate with 48 and 45% of cells, respectively, completing three cell cycles in 48 h. Bone marrow cells cultured in the CFU-GM assay showed the slowest proliferative activity with only 1% of third division metaphases at 48 h.
Crossen
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Bodger
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Trent
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D001854:Bone Marrow Cells; D002453:Cell Cycle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003470:Culture Media; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D013194:Staining and Labeling; D013997:Time Factors; D014410:Tumor Stem Cell Assay
D003470:Culture Media