Expression of plasmid encoded Escherichia coli 5S ribosomal ribonucleic acid in Pseudomonas putida.
The recombinant plasmid pNRK 36, which represents the plasmids RSF 1010, a small multicopy plasmid of the incompatibility group IncQ that confers resistance to streptomycin and sulfoamide to its host cells, and pKK 223-3, which contains the try-lac (tac) promoter followed by a polylinker and a DNA segment containing the 5S rRNA (rrn B) with the ribosomal RNA transcription terminators, was employed to transform Pseudomonas putida 2440 cells. The plasmid encoded 5 S rRNA from Escherichia coli was transcribed and processed properly in P. putida cells thus demonstrating for the first time the expression of a plasmid encoded ribosomal RNA in a heterologous system. The fact that the E. coli 5 S rRNA was not incorporated into assembled ribosomes suggests that the in vivo incorporation of 5 S rRNA into the 50 S ribosomal subunit is closely linked to 23 S rRNA and/or ribosomal protein synthesis.
R K
RK
Hartmann
P P
PP
Henze
N
N
Ulbrich
V A
VA
Erdmann
D002499:Centrifugation, Density Gradient; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005815:Genetic Code; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics*; D012329:RNA, Bacterial / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D014158:Transcription, Genetic; D014169:Transformation, Bacterial
D012329:RNA, Bacterial; D012335:RNA, Ribosomal