An improved method for identifying 8-O-acetyl-N-acetylneuraminic acid.
The periodic acid/thionin-Schiff/potassium hydroxide/periodic acid/fuchsin-Schiff sequence developed by Culling et al. frequently causes damage to sections and gives inconsistent results because of insufficient primary oxidation and difficulties in making the thionin-Schiff reagent. These disadvantages have been largely eliminated by more thorough primary oxidation and by replacing the original thionin-Schiff with a new cold thionin-Schiff. The effect of alkaline hydrolysis on thionin-aldehyde complexes was also studied and the reduction of color caused by this treatment was restored by a second thionin-Schiff reaction. The new sequence gives consistent results and imparts greater color to the thionin-Schiff reaction.
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M
Hayama
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K
Ono
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T
Katsuyama
D003110:Colonic Neoplasms / Q000032:analysis; D006651:Histocytochemistry; D006801:Humans; D010503:Periodic Acid-Schiff Reaction; D012794:Sialic Acids / Q000032:analysis*; D013194:Staining and Labeling / Q000379:methods*; D013274:Stomach Neoplasms / Q000032:analysis
D012794:Sialic Acids; C046053:8-O-acetyl-N-acetylneuraminic acid