Prolactin stimulates transcription of growth-related genes in Nb2 T lymphoma cells.
The pituitary peptide hormone prolactin exerts a profound effect on various physiological processes involving both cellular proliferation and differentiation. The rat Nb2 T lymphoma cell line has been used as a model system for studying prolactin regulation of cell proliferation. Several genes associated with cell growth (c-myc, ornithine decarboxylase (ODC), heat shock protein 70 (hsp 70)-homologue, and beta-actin) are induced rapidly within 4 h after prolactin addition. Nuclear run-on transcription assays indicate that prolactin induction of these growth-related genes occurs primarily at the transcriptional level. According to the different kinetics of transcriptional response to prolactin, these growth-related genes can be divided into immediate-early (actin, c-myc), early (ODC) and mid-G1 (hsp 70-homologue) genes. Thus, prolactin may regulate Nb2 T cell-proliferative responses by modulating the transcriptional induction of various growth-related genes. These studies also represent a first report of a transcriptional cascade set off in rapid response to prolactin in cultured T cells.
Department of Medicine, Baylor College of Medicine, Houston, TX 77030.
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Yu-Lee
D000199:Actins / Q000096:biosynthesis / Q000235:genetics*; D000818:Animals; D015152:Blotting, Northern; D002455:Cell Division / Q000187:drug effects*; D005786:Gene Expression Regulation; D005796:Genes / Q000187:drug effects*; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D008223:Lymphoma; D051379:Mice; D009955:Ornithine Decarboxylase / Q000096:biosynthesis / Q000235:genetics*; D011388:Prolactin / Q000494:pharmacology*; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc; D051381:Rats; D014158:Transcription, Genetic / Q000187:drug effects*; D014407:Tumor Cells, Cultured
D000199:Actins; D006360:Heat-Shock Proteins; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D011388:Prolactin; D009955:Ornithine Decarboxylase