Secondary structure of the murine histocompatibility alloantigen H-2Kb: relationship between heavy chain, beta 2-microglobulin, and antigenic reactivity.
The far-ultraviolet circular dichroism (CD) spectra of the extracellular portion (papain-cleaved fragment) of the histocompatibility antigen H-2Kb and its noncovalently associated components, heavy chain and beta 2-microglobulin (beta 2m), indicate that the antigen is highly structured, containing about 30% alpha-helix, 41% beta-sheet, and 29% random coil. Separation of beta 2m from the heavy chain produced a decrease in heavy chain alpha-helix and beta-sheet structure which correlated with a loss of alloantigenic reactivity. Reconstitution of the heavy chain-beta 2m complex resulted in an increase in secondary structure which was greater than the sum of the free chains and the recovery of considerable alloantigenic reactivity. This suggests that some of the secondary structure and much of the alloantigenic reactivity may depend on conformation associated with the binding of beta 2m to heavy chain. A prediction of heavy chain secondary structure based on Chou-Fasman analysis of the primary amino acid sequence agreed with results from CD measurements and suggested that the segments of alpha-helix and beta-sheet structure are distributed throughout the molecule.
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Yokoyama
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Geier
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Uehara
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Nathenson
D000818:Animals; D002942:Circular Dichroism; D000939:Epitopes / Q000032:analysis; D006183:H-2 Antigens / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000276:immunology; D007519:Isoantigens; D051379:Mice; D008958:Models, Molecular; D010206:Papain; D010446:Peptide Fragments / Q000032:analysis; D011487:Protein Conformation; D001613:beta 2-Microglobulin / Q000276:immunology
D000939:Epitopes; D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D007143:Immunoglobulin Heavy Chains; D007519:Isoantigens; D010446:Peptide Fragments; D001613:beta 2-Microglobulin; D010206:Papain