Differences in PCR reactivity between HIV proviruses from individuals in Ethiopia and Sweden.
The polymerase chain reaction (PCR), using primer pairs in the gag, pol, and env regions, was used in a comparative study of HIV-1 DNA in peripheral mononuclear blood cells from HIV-1-seropositive individuals in Ethiopia and Sweden. Although all Swedish samples were positive by PCR, the reactivity was more pronounced in samples from late stages than in those from early stages of infection. Six of nine Ethiopian samples from HIV-1-seropositive patients were positive by PCR, but the reactions were much weaker than those observed for Swedish samples, and in most cases seen with one primer pair only. These results suggest that the burden of HIV-1 DNA in peripheral mononuclear blood cells increases with advancing disease. PCR using primer pairs designed to detect HIV-1 infection in Europe and North America is not always suitable for the detection of HIV-1 infection in Ethiopia. The differences in PCR reactivity could possibly be a consequence of differences regarding host responses to the virus in the two countries, but more likely due to genomic differences between HIV-1 strains prevalent in Ethiopia and Sweden.
Ayehunie
S
S
Department of Biology, Addis Ababa University, Ethiopia.
Sönnerborg
A
A
Johansson
B
B
Fehniger
T E
TE
Zewdie
D W
DW
Yeamane-Berhan
T
T
Petros
B
B
Abens
J
J
Britton
S
S
Strannegård
O
O
D000386:AIDS-Related Complex / Q000382:microbiology; D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology; D004279:DNA, Viral / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D005002:Ethiopia; D005784:Gene Amplification; D015658:HIV Infections / Q000382:microbiology*; D006679:HIV Seropositivity / Q000382:microbiology; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D015151:Immunoblotting; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics*; D013548:Sweden
D004279:DNA, Viral