Variation of the poly(A) size classes in the rat mammary gland during the lactation cycle.
The sizes of the poly(A) tracts associated with rat mammary RNA were determined at several time points in the lactation cycle. The poly(A) tracts in the lactating gland displayed two predominant size class peaks at 80-85 and 45-47 residues. The 9S whey protein mRNA and the 15S casein mRNA purified from the 12 day lactating mammary gland both contained poly(A) tracts displaying a similar size distribution. The 45 residue tracts were a characteristic of lactation; they were not found at 8 days of pregnancy and only small amounts of these shorter poly(A) tracts were found in the 16 day pregnant gland. The poly(A) tracts of the involuted gland displayed the same size characteristics as those of late pregnancy. At all the developmental stages that were examined, the fraction of 45 residue poly(A) tracts was always proportional to the total poly(A) content of the mammary cells.
S A
SA
Nadin-Davis
V A
VA
Mezl
D000818:Animals; D002364:Caseins / Q000235:genetics; D005260:Female; D007774:Lactation; D008321:Mammary Glands, Animal / Q000032:analysis*; D008894:Milk Proteins / Q000235:genetics; D011061:Poly A / Q000032:analysis*; D011247:Pregnancy; D012313:RNA / Q000032:analysis*; D012333:RNA, Messenger; D051381:Rats; D000067816:Whey Proteins
D002364:Caseins; D008894:Milk Proteins; D012333:RNA, Messenger; D000067816:Whey Proteins; D011061:Poly A; D012313:RNA