Effect of alpha-fetoprotein on arachidonic acid metabolism in the preadipocyte cell line OB 17.
Previous work has shown that alpha-fetoprotein (AFP) is able to bind specifically polyunsaturated fatty acids, one of the major ligands being arachidonic acid (C20:4). In the present study, we demonstrate that AFP is able to reduce the metabolism of exogenous C20:4 by ob17 cells. Both prostaglandins and lipoxygenase products formation were reduced when cells were maintained in the presence of AFP. This decrease was counterbalanced by a higher release of C20:4 into the culture medium by the cells. The amount of C20:4 incorporated into cellular lipids was decreased but the distribution of C20:4 in the different lipid classes remained unchanged. The modification of C20:4 metabolism by AFP might be of primordial importance in developmental biology and may shed a new light on the physiological actions of AFP which have been described in the past years such as ovarian inhibition, cell growth control and immuno-suppressive activity.
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Aussel
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Negrel
D000273:Adipose Tissue / Q000187:drug effects; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D001692:Biological Transport; D002460:Cell Line; D003470:Culture Media; D007700:Kinetics; D011453:Prostaglandins / Q000096:biosynthesis; D051381:Rats; D014316:Tritium; D000509:alpha-Fetoproteins / Q000494:pharmacology*
D001095:Arachidonic Acids; D003470:Culture Media; D011453:Prostaglandins; D000509:alpha-Fetoproteins; D014316:Tritium; D016718:Arachidonic Acid