Isolation of a cDNA clone for a catalytic subunit of Torpedo marmorata acetylcholinesterase.
We have constructed a cDNA library from Torpedo marmorata electric organ poly(A+) RNA in the lambda phage expression vector lambda gt11. This library has been screened with polyclonal anti-acetylcholinesterase antibodies. One clone, lambda AChE1, produced a fusion protein which was recognized by the antibodies and which prevented the binding of native acetylcholinesterase in an enzymatic immune assay. These results indicate that lambda AChE1 contains a cDNA insert coding for a part of a catalytic subunit of Torpedo acetylcholinesterase. The 200-base-pair cDNA insert hybridized to three mRNAs (14.5, 10.5 and 5.5 kb) from Torpedo electric organs. These mRNAs were also detected in Torpedo electric lobes.
J L
JL
Sikorav
F
F
Vallette
J
J
Grassi
J
J
Massoulié
D000110:Acetylcholinesterase / Q000235:genetics*; D000818:Animals; D002384:Catalysis; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D007120:Immunochemistry; D009693:Nucleic Acid Hybridization; D011061:Poly A; D012313:RNA; D012333:RNA, Messenger; D014101:Torpedo
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA; D000110:Acetylcholinesterase