Determination of gangliosides in six human primary medulloblastomas.
The ganglioside composition of six human medulloblastomas was analyzed. The characterization was performed by thin-layer chromatography, sialidase hydrolysis, and immunological staining with a panel of characterized antiganglioside monoclonal antibodies. The total ganglioside content ranged from 60 to 1,130 nmol of ganglioside sialic acid/g wet weight. Neuronal gangliosides (gangliotetraose series) were found in varying amounts in all medulloblastomas. Gangliosides of the neolactotetraose series (3'-LM1 and LD1) were present in all specimens, and the lactotetraose series ganglioside 3'-isoLM1 was found in all cases showing astrocytic differentiation. This supports our previous findings that 3'-isoLM1 is a marker for proliferating astroglial cells.
Department of Psychiatry and Neurochemistry, St. Jörgen Hospital, Hisings Backa, Sweden.
J
J
Gottfries
P
P
Fredman
J E
JE
Månsson
V P
VP
Collins
H
H
von Holst
D D
DD
Armstrong
A K
AK
Percy
C J
CJ
Wikstrand
D D
DD
Bigner
L
L
Svennerholm
D000293:Adolescent; D002528:Cerebellar Neoplasms / Q000032:analysis* / Q000601:surgery; D002648:Child; D002675:Child, Preschool; D002855:Chromatography, Thin Layer; D005260:Female; D005732:Gangliosides / Q000032:analysis* / Q000302:isolation & purification; D006801:Humans; D007223:Infant; D008297:Male; D008527:Medulloblastoma / Q000032:analysis* / Q000601:surgery; D012794:Sialic Acids / Q000032:analysis
D005732:Gangliosides; D012794:Sialic Acids