Murine endodermal cytokeratins Endo A and Endo B are localized in the same intermediate filament.
The intracellular distribution of extra-embryonic endodermal, cytoskeletal proteins A (Endo A) and B (Endo B) was investigated by double-label immunofluorescent microscopy and double-label immunoelectron microscopy. In parietal endodermal cells, the immunofluorescent distribution of Endo B was always coincident with that of Endo A and could be distinguished from vimentin, particularly at the periphery of the cell. At the electron microscopic level, antibodies against both Endo A and Endo B recognized both bundles and individual intermediate filaments. Double-label immunoelectron microscopy was achieved by use of two sizes of colloidal gold particles (5 nm and 20 nm) that were stabilized with secondary antibodies. These results show that Endo A and B are found in the same intermediate filament and probably co-polymerize to form such structures.
W E
WE
Howe
F G
FG
Klier
R G
RG
Oshima
D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D003599:Cytoskeleton / Q000032:analysis*; D004707:Endoderm / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D006046:Gold; D007382:Intermediate Filaments / Q000032:analysis*; D007633:Keratins / Q000032:analysis*; D051379:Mice; D008854:Microscopy, Electron; D051381:Rats; D014746:Vimentin / Q000032:analysis
D000911:Antibodies, Monoclonal; D014746:Vimentin; D007633:Keratins; D006046:Gold