Enzyme-linked immunosorbent assay of substance P and its metabolite SP 1-7. A comparison with RIA.
We have investigated enzyme-linked immunosorbent assay (ELISA) as a possible alternative to radioimmunoassay (RIA) for the detection and measurement of the neuropeptide substance P (SP) and its metabolite substance P1-7. The sensitivities were higher with ELISA than with RIA utilizing the same antisera. The intra-assay and inter-assay variation in ELISA was 1% and 13% respectively. The higher sensitivities are in part due to the standard curves having less steep slopes, and in part to lower IC50s in the ELISA. Since there was a good correlation between peptide levels in biological samples as determined by ELISA and RIA respectively, ELISA might be considered an attractive alternative to RIA.
Folkesson
R
R
Neil
A
A
Terenius
L
L
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D010446:Peptide Fragments / Q000032:analysis*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013373:Substance P / Q000032:analysis*
D010446:Peptide Fragments; D013373:Substance P; C036877:substance P (1-7)