Ruthenium red-insensitive Ca2+ uptake and release by mitochondria.
Calcium uptake by rat liver mitochondria driven by an artificial pH gradient is ruthenium red insensitive, electrically neutral, and inhibited by the local anesthetic, nupercaine. This pH-driven Ca2+ transport is also inhibited by NH3, Pi, and acetate. Direct measurements of Pi indicate it is not translocated with Ca2+ during pH-driven Ca2+ uptake. Calcium is therefore not transported by a Ca2+-Pi symport mechanism. Ruthenium red-insensitive Ca2+ efflux is similar in its inhibition by nupercaine and its kinetics, and is also electroneutral. This suggests that the Ca2+ uptake described here occurs via reversal of the principal pathway of mitochondrial Ca2+ release. From the available data, pH-driven Ca2+ uptake (and presumably Ca2+ efflux) is hypothesized to occur by Ca2+ symport with unidentified anions. Protons may move counter to Ca2+ or reversibly dissociate from cotransported anions, which therefore couples Ca2+ transport to the pH gradient.
Cockrell
R S
RS
D000617:Aminoglycosides; D000818:Animals; D000900:Anti-Bacterial Agents; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism; D003992:Dibucaine / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D006027:Glycosides / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008274:Magnesium / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects; D011188:Potassium / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012428:Ruthenium / Q000494:pharmacology*; D012430:Ruthenium Red / Q000494:pharmacology*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D002712:Chlorides; D006027:Glycosides; D012430:Ruthenium Red; C000904:dianemycin; D012428:Ruthenium; D008274:Magnesium; D003992:Dibucaine; D005033:Ethylmaleimide; D011188:Potassium; D002118:Calcium