Labeling of cutaneous sensory nerve endings with axonally transported horseradish peroxidase and wheat germ agglutinin-horseradish peroxidase conjugate: a methodological study in the rat.
Practical aspects on the use of horseradish peroxidase (HRP) and wheat germ agglutinin-horseradish peroxidase conjugate (WGA-HRP) to trace peripheral cutaneous nerve endings have been studied. The parameters studied included application site of the tracer, post-application survival time, tracer concentration and tracer volume. These parameters were examined in the glabrous skin of the rat hindpaw. The best results were obtained with injections of 1 microliter WGA-HRP (20 micrograms/microliter) in dorsal root ganglia innervating the examined cutaneous region and a postinjection survival time of 18-36 h. With this approach extensive and heavy labeling was achieved of epidermal nerve endings, nerve endings in Merkel cell-neurite complexes and Meissner corpuscles. Useful, but less extensive labeling of these types of peripheral nerve endings, was obtained with injections of HRP in the lumbar spinal cord dorsal horn.
H
H
Aldskogius
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E
Kinnman
J
J
Persson
D000818:Animals; D001370:Axonal Transport; D002470:Cell Survival; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology; D006735:Horseradish Peroxidase; D037102:Lectins; D009475:Neurons, Afferent / Q000032:analysis* / Q000502:physiology; D010544:Peroxidases; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000294:innervation*; D013116:Spinal Cord / Q000032:analysis; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate
D037102:Lectins; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D006735:Horseradish Peroxidase; D010544:Peroxidases