Plasma and tissue-free amino acids in the developing chick.
The free amino acids of the plasma and pectoral muscle, liver and brain cytosolic S105 supernatant obtained from 2- and 3-week-old fasted chicks, were analyzed by HPLC after deproteinization by ultrafiltration. Protein-bound amino acids were analyzed in parallel studies. A clear relationship between the free and bound tissue amino acids was apparent. Only for the gluconeogenic amino acids a close correlation was evident between the plasma and the muscle free amino acids. A relatively steady composition of the free amino acids is established early in the tissues, but in the plasma it changes significantly with age.
Laboratoire de Physiologie Métabolique et Nutrition, Université Pierre-et-Marie-Curie, Paris, France.
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D000375:Aging / Q000378:metabolism; D000596:Amino Acids / Q000097:blood / Q000378:metabolism*; D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D003600:Cytosol / Q000378:metabolism; D014462:Ultrafiltration
D000596:Amino Acids