The role of 5-lipoxygenase pathway activation in basophil histamine release.
This study was performed to evaluate the role of products of the 5-lipoxygenase pathway in histamine release from human basophils. AA-861, a specific inhibitor of 5-lipoxygenase, did not inhibit anti-IgE-induced histamine release from human basophils with 6 +/- 11% inhibition at 10(-6)M. Neither was there a marked inhibition of histamine release induced by ionophore A23187 and formyl-L-methionyl-L-leucyl-L-phenylalanine with 16 +/- 10 and 11 +/- 6% inhibition respectively at 10(-6)M of AA-861. In contrast, AA-861 enhanced 12-0-tetradecanoyl-phorbol-13-acetate-induced histamine release with 20 +/- 3% at 10(-6)M. These results suggest that 5-lipoxygenase activation may not play a role in basophil histamine release.
Morita
Y
Y
Suzuki
S
S
Miyamoto
T
T
D001491:Basophils / Q000201:enzymology / Q000276:immunology*; D016227:Benzoquinones; D004789:Enzyme Activation; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism; D008084:Lipoxygenase / Q000378:metabolism*; D011809:Quinones / Q000494:pharmacology; D013189:SRS-A / Q000096:biosynthesis
D016227:Benzoquinones; D011809:Quinones; D013189:SRS-A; C036837:2,3,5-trimethyl-6-(12-hydroxy-5,10-dodecadiynyl)-1,4-benzoquinone; D008084:Lipoxygenase