Subcellular localization of tissue polypeptide antigen and cytokeratins in epithelial cells (salivary ad mammary glands). Combined use of the cryoultramicrotomy and the protein A-gold technique.
Epithelial cells from various sites and at various stages of differentiation reveal distinct cytokeratin polypeptide patterns. WE have localized these heterogeneous elements at the subcellular level in human salivary glands and in a solid tumor of the breast using a monoclonal and a polyclonal antibody against cytokeratin, and an antibody against tissue polypeptide antigen (TPA) which seems to be related to some cytokeratins. Labeling by the cytokeratin antibodies was more intense in squamous and duct cells than in acinar cells. The TPA:B1 antibody reacted predominantly with duct cells and to a lesser extent with acinar and squamous cells. A precise evaluation of the labeling pattern and a well-preserved cell structure appeared to be important factors in obtaining more detailed information about intermediate filament proteins. The cryoultramicrotomy and the protein A-gold technique are suitable for these studies.
K P
KP
Zimmer
J
J
Caselitz
G
G
Seifert
D001940:Breast / Q000378:metabolism*; D003896:Desmosomes / Q000378:metabolism; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005629:Frozen Sections; D006046:Gold; D006652:Histological Techniques; D006801:Humans; D007382:Intermediate Filaments / Q000378:metabolism; D007633:Keratins / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D012469:Salivary Glands / Q000166:cytology / Q000378:metabolism*; D013205:Staphylococcal Protein A; D014018:Tissue Distribution; D019396:Tissue Polypeptide Antigen
D010455:Peptides; D013205:Staphylococcal Protein A; D019396:Tissue Polypeptide Antigen; D007633:Keratins; D006046:Gold