The pH dependence of the hemolytic potency of bile salts.
The membrane damaging potential of dilute solutions of bile salts was evaluated by monitoring continuously the hemolysis of a small sample of red blood cells (RBC) introduced into a defined media containing the bile salts at various pH values. The strength of the hemolytic bile salt was characterized by the rate of the induced hemolysis and by the time that elapsed between the introduction of the RBC sample into the bile salt containing solution and the onset of hemolysis. The potency of the unconjugated bile acids was extremely sensitive to pH, e.g. the rate of hemolysis caused by a 7.5 mM cholate was 1.5%, 20% and 64% per min when the pH of the solution was 7.65, 7.3 and 6.85, respectively. At low pH values the membrane damaging effects of deoxycholate was clearly discerned at micromolar concentration range. The hemolytic potency of glycodeoxycholate was also enhanced significantly by lowering the pH. The taurine-conjugated cholate and deoxycholate were only slightly sensitive to variations in pH. Taurocholate at concentrations that were not hemolytic greatly enhanced the injurious potency of deoxycholate. These results imply that in acidic solutions the presence of bile acids can cause damage to cell membranes. It is suggested that the acidic environment in the proximal duodenum and acidosis developed during hypoxia in the liver are two situations in which the bile salts may constitute a pathogenic factor.
Department of Physiology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
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Ilani
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Granoth
D000818:Animals; D001647:Bile Acids and Salts / Q000494:pharmacology*; D002635:Chenodeoxycholic Acid / Q000494:pharmacology; D019826:Cholic Acid; D002793:Cholic Acids / Q000494:pharmacology; D003840:Deoxycholic Acid / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects / Q000502:physiology; D006461:Hemolysis / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D051381:Rats; D013329:Structure-Activity Relationship; D014580:Ursodeoxycholic Acid / Q000494:pharmacology
D001647:Bile Acids and Salts; D002793:Cholic Acids; D003840:Deoxycholic Acid; D002635:Chenodeoxycholic Acid; D014580:Ursodeoxycholic Acid; D019826:Cholic Acid