Incompatible A antigen expressed in tumors of blood group O individuals: immunochemical, immunohistologic, and enzymatic characterization.
Monofucosyl type 1 chain A (type 1 Aa) and difucosyl type 1 chain A (ALeb), but not other types of A antigens, have been detected by application of carrier type-specific monoclonal anti-A antibodies (AH21 and HH3) in colonic tumors of blood group O individuals. An A-transferase activity (UDP-Gal-NAc:H-alpha-GalNAc transferase) was demonstrated in the extract of one of the O tumors expressing A antigen. The incidence of A antigen expression in O tumors was found to be two out of 15 cases, based on TLC immunostaining of glycolipid extracts, and five out of 50 cases, based on immunofluorescent staining of tumors with AH21 and HH3 antibodies.
Clausen
H
H
Hakomori
S
S
Graem
N
N
Dabelsteen
E
E
D000017:ABO Blood-Group System / Q000276:immunology; D000230:Adenocarcinoma / Q000097:blood* / Q000201:enzymology / Q000473:pathology; D000911:Antibodies, Monoclonal; D001787:Blood Group Incompatibility / Q000097:blood* / Q000201:enzymology / Q000473:pathology; D002855:Chromatography, Thin Layer; D005455:Fluorescent Antibody Technique; D005700:Galactosyltransferases / Q000276:immunology / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood* / Q000201:enzymology / Q000473:pathology; D017350:N-Acetylgalactosaminyltransferases; D013194:Staining and Labeling
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D005700:Galactosyltransferases; D017350:N-Acetylgalactosaminyltransferases; C003742:UDPgalactosamine-galactose acetylgalactosaminyltransferase