The use of vital dyes to assess embryonic viability in the hamster, Mesocricetus auratus.
Experiments were designed to assess the use of the vital dyes trypan blue and fluorescein diacetate as indicators of the viability of hamster ova and embryos. Exclusion of trypan blue and fluorescence with fluorescein diacetate showed high correlations with uptake of [3H]uridine by ova and further development of embryos in vitro. Ova killed by freezing and thawing incorporated [3H]uridine at background levels. Trypan blue exclusion and fluorescein diacetate uptake were highly correlated with each other (r = 0.99). Trypan blue and fluorescein diacetate serve as excellent indices of viability in ova and early embryos of hamsters.
R J
RJ
Hutz
F J
FJ
DeMayo
W R
WR
Dukelow
D000818:Animals; D002470:Cell Survival; D006224:Cricetinae; D004622:Embryo, Mammalian / Q000502:physiology*; D005260:Female; D005452:Fluoresceins; D008647:Mesocricetus; D010063:Ovum / Q000378:metabolism / Q000502:physiology*; D013194:Staining and Labeling; D014316:Tritium; D014343:Trypan Blue; D014529:Uridine
D005452:Fluoresceins; D014316:Tritium; D014343:Trypan Blue; D014529:Uridine; C018506:diacetylfluorescein