Determination of thiamazole in serum by high-performance liquid chromatography with electrochemical detection.
A simple and sensitive method for the determination of thiamazole in serum by high-performance liquid chromatography with electrochemical detection is described. Thiamazole in serum was quantified without an extraction procedure at concentrations down to 10 ng/ml. This method was applied to determine the serum concentration of the drug in two healthy volunteers given a single oral dose of 10 mg of thiamazole. The concentration of the drug reached a maximum at 3-4 h after the oral dose and two elimination phases were observed.
Tatsuhara
T
T
Tabuchi
F
F
Unate
M
M
Okamura
Y
Y
Shigemasa
C
C
Abe
K
K
Mashiba
H
H
D000284:Administration, Oral; D013956:Antithyroid Agents / Q000008:administration & dosage / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D006801:Humans; D007700:Kinetics; D008297:Male; D008713:Methimazole / Q000008:administration & dosage / Q000097:blood*
D013956:Antithyroid Agents; D008713:Methimazole