Serological cross-reactions between Brucella abortus and Yersinia enterocolitica 0:9.
Yersinia enterocolitica (Ye) 0:9 is an organism of great significance in veterinary medicine largely as a result of its cross-reaction with Brucella abortus (Ba). Boty Ye 0:9 and Ba possess somatic antigens in common; as a result of which animals exposed to Ye 0:9 have an immune response which is distinguishable only with difficulty from that induced by Ba. Cattle were exposed to Ye 0:9 by the oral or intramammary routes. Oral exposure failed to generate significant serologic response. In contrast, intramammary inoculation produced a marked response. Serum antibodies provoked in this manner reacted strongly with Ba. The anti-Brucella response provoked by inoculation of Yersinia was sufficient to render milk and serum Brucella-seropositive as measured by the standard milk ring and serum agglutination tests. While both Ba and Ye 0:9 have 9 antigens in common, they differ significantly with respect to motility. Thus Ba is always non motile while Ye is motile when grown at room temperature. The presence of Yersinia H agglutinins in serum were shown to be evidence of previous exposure to Ye. The H agglutinins were not generated by Brucella infection. A rapid H agglutination test was shown to provide this differentiation without interference from cross-reacting O antigens. Results of Ba O and Ye O and OH antigens used in the agglutination test were found useful to differentiate antibodies against Ba from those induced by Ye 0:9 in cattle sera. The existence of enterobacterial common antigen (ECA) in Ye and its absence in Ba were utilized in an attempt to provide a method to distinguish Brucella infections from those with cross-reacting Yersinia.(ABSTRACT TRUNCATED AT 250 WORDS)
Mittal
K R
KR
Tizard
I R
IR
Barnum
D A
DA
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D002003:Brucella abortus / Q000276:immunology*; D002007:Brucellosis, Bovine / Q000175:diagnosis* / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000382:microbiology; D003429:Cross Reactions; D003937:Diagnosis, Differential; D005189:False Positive Reactions; D019081:O Antigens; D015009:Yersinia Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D015008:Yersinia enterocolitica / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D019081:O Antigens; C017286:enterobacterial common antigen