Enhancement of interleukin-2 activity by liposomes.
The present report demonstrates that liposomes increase the interleukin-2 (IL-2) dependent proliferation of cytotoxic T-lymphocyte line (CTLL) cells used for the measurement of IL-2 activity. This effect was better observed with suboptimal doses of IL-2 and low concentrations of lipids. The increased IL-2 dependent proliferation is not due to a direct effect of liposomes on CTLL cells but rather to an interaction between IL-2 and liposomes. An interaction between IL-2 and components of fetal calf serum is also demonstrated. The results indicate that liposomes may interfere with IL-2 bioassay but also show the possibility of potentiating IL-2 activity for therapeutic purposes.
Unité de la Rage, Institut Pasteur, Paris, France.
M L
ML
Joffret
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S
Morgeaux
C
C
Laclerc
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D
Oth
C
C
Zanetti
P
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Sureau
P
P
Perrin
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D002417:Cattle; D002850:Chromatography, Gel; D004347:Drug Interactions; D005260:Female; D007376:Interleukin-2 / Q000276:immunology* / Q000494:pharmacology; D008081:Liposomes / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D011820:Rabies virus / Q000276:immunology; D011994:Recombinant Proteins / Q000494:pharmacology; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology
D000956:Antigens, Viral; D007376:Interleukin-2; D008081:Liposomes; D011994:Recombinant Proteins