Repeated determination of cerebrospinal fluid amine metabolites by automated direct sampling from an implanted cannula in freely moving rats.
A method is described for the automatic sampling of CSF from conscious freely moving rats and the simultaneous determination of 5-HIAA and HVA by high-pressure liquid chromatography with electrochemical detection (HPLC-EC). Timed samples (30 microliters) were taken directly from a chronically implanted cannula in the cisterna magna of adult female rats by the HPLC autoinjector. The low volume of sample permitted withdrawal of repeated samples without cross-contamination. The cannula could be used the day after implantation and remained patent for at least two weeks. Treatment with probenecid (200 mg/kg i.p.) caused an increase of both metabolites in the CSF. Pargyline (50 mg/kg i.p.) affected both the 5-HT and dopaminergic systems, reducing their metabolite levels. alpha-Methyl-p-tyrosine (200 mg/kg i.p.) acted selectively on the catecholaminergic system lowering HVA levels. The method accurately measured amine metabolite changes in the CSF due to pharmacological manipulations.
C F
CF
De La Riva
J A
JA
Yeo
D000818:Animals; D001679:Biogenic Amines / Q000134:cerebrospinal fluid*; D002408:Catheters, Indwelling; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006719:Homovanillic Acid / Q000134:cerebrospinal fluid; D006897:Hydroxyindoleacetic Acid / Q000134:cerebrospinal fluid; D008781:Methyltyrosines / Q000494:pharmacology; D010293:Pargyline / Q000494:pharmacology; D011339:Probenecid / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013129:Spinal Puncture / Q000379:methods*; D019805:alpha-Methyltyrosine
D001679:Biogenic Amines; D008781:Methyltyrosines; D006897:Hydroxyindoleacetic Acid; D019805:alpha-Methyltyrosine; D010293:Pargyline; D011339:Probenecid; D006719:Homovanillic Acid