The potentiation of "incomplete" mouse monoclonal anti-Lea agglutination with colloids.
Mouse monoclonal anti-Lea produced in this center was found to agglutinate only Le(a+) red cells which had been modified with proteases. In order to provide a stable medium in which the monoclonal anti-Lea would directly agglutinate unmodified red cells, the potentiating effect of a variety of colloids was assessed. Satisfactory results were obtained when 10 percent (wt/vol) dextran and 2 percent (wt/vol) bovine serum albumin were added to the culture supernatant containing anti-Lea. It is suggested that direct hemagglutination mediated by these colloids resulted from a combination of increased antibody uptake by Le(a+) red cells and change in the shape of these red cells induced by dextran.
G
G
Inglis
R H
RH
Fraser
M A
MA
Durosinmi
E K
EK
Allan
A
A
Mackie
R
R
Mitchell
D000911:Antibodies, Monoclonal / Q000276:immunology*; D001666:Binding Sites, Antibody; D003102:Colloids; D003911:Dextrans / Q000494:pharmacology*; D004906:Erythrocyte Count; D004912:Erythrocytes; D006384:Hemagglutination / Q000187:drug effects*; D006801:Humans; D007518:Isoantibodies / Q000276:immunology*; D007983:Lewis Blood Group Antigens; D012709:Serum Albumin / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D003102:Colloids; D003911:Dextrans; D007518:Isoantibodies; D007983:Lewis Blood Group Antigens; D012709:Serum Albumin