Measurement of 5-aminolaevulinate synthase activity by gas-liquid chromatography with electron-capture detection.
A specific assay for 5-aminolaevulinate synthase activity is described, with a sensitivity comparable with that of radiochemical assays. It is based on measurement by g.l.c. with electron-capture detection of the pentafluorobenzyl ester of the ethyl acetoacetate pyrrole derivative of 5-aminolaevulinic acid, and of the corresponding compound from 6-amino-5-oxohexanoic acid used as internal standard. Enzyme activity has been measured in homogenates of rat liver, spleen, kidney and brain, and in human lymphocytes.
Department of Clinical Pharmacology, St. Mary's Hospital Medical School, London, U.K.
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Gorchein
D000624:5-Aminolevulinate Synthetase / Q000032:analysis*; D000818:Animals; D001921:Brain / Q000201:enzymology; D002849:Chromatography, Gas / Q000379:methods; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008214:Lymphocytes / Q000201:enzymology; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains; D013154:Spleen / Q000201:enzymology
D000624:5-Aminolevulinate Synthetase