Cerulein-induced pancreatitis in rats: increased lysosomal enzyme activity and autophagocytosis.
The involvement of lysosomes in the normal secretory process of the exocrine pancreas and in the onset of acute, hormone-induced pancreatitis was studied. The enzymatic activities of cathepsin B and beta-galactosidase were determined in the pancreas of rats that had been stimulated by either maximal (0.25 microgram X kg-1 h-1) or supramaximal (5 micrograms X kg-1 h-1) concentrations of cerulein. Maximal stimulation led to a moderate increase in cathepsin B activity and the ultrastructural appearance of multivesicular bodies. Supramaximal stimulation resulted in formation of large cytoplasmic vacuoles and progressive destruction of acinar cells which was paralleled by a marked increase of lysosomal enzyme activity.
G
G
Adler
C
C
Hahn
H F
HF
Kern
K N
KN
Rao
D000681:Amylases / Q000097:blood; D000818:Animals; D001343:Autophagy; D002401:Cathepsin B; D002403:Cathepsins / Q000378:metabolism*; D002108:Ceruletide; D006868:Hydrolysis; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D010195:Pancreatitis / Q000139:chemically induced* / Q000201:enzymology; D010587:Phagocytosis; D051381:Rats; D011919:Rats, Inbred Strains; D001616:beta-Galactosidase / Q000378:metabolism
D002108:Ceruletide; D000681:Amylases; D001616:beta-Galactosidase; D002403:Cathepsins; D002401:Cathepsin B