Synthesis of a myelin-like membrane by oligodendrocytes in culture.
We have prepared highly purified cultures of rat oligodendrocytes by a modification of the procedure of McCarthy and de Vellis [1980]. By utilizing a substratum derived from lysed glia and a calf serum-containing medium with a high concentration of transferrin, the oligodendrocyte cultures display a high degree of purity, the ability to survive several months of culture, and a striking ability to produce a myelin-like membrane. We have examined the production of this myelin-like membrane using immunocytochemical and biochemical probes as well as an extensive morphological examination at the electron microscopic level. The membrane appears to be produced in a similar developmental pattern to that observed in vivo and it has the structural characteristics of loosely packed central nervous system myelin.
Rome
L H
LH
Bullock
P N
PN
Chiappelli
F
F
Cardwell
M
M
Adinolfi
A M
AM
Swanson
D
D
D000818:Animals; D001921:Brain / Q000378:metabolism; D002458:Cell Fractionation; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D002478:Cells, Cultured; D005699:Galactosylceramides / Q000032:analysis; D006017:Glycolipids / Q000378:metabolism; D008854:Microscopy, Electron; D004676:Myelin Basic Protein / Q000032:analysis; D009186:Myelin Sheath / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D009457:Neuroglia / Q000378:metabolism*; D009836:Oligodendroglia / Q000378:metabolism*; D010168:Palmitates / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling
D005699:Galactosylceramides; D006017:Glycolipids; D004676:Myelin Basic Protein; D010168:Palmitates