A method for analysis of cell morphology, banded karyotype, and immunoperoxidase identification of lymphocyte subset on the same cell.
The authors describe a method that allows three staining procedures to be applied to the same mitotic cell, namely Giemsa staining for morphology, immunoperoxidase staining for surface markers, and banding of the chromosomes for karyotype. After culturing of the blood lymphocytes, colchicine was added to arrest the cells in mitosis. After mild hypotonic treatment, the cells were cytocentrifuged to glass slides. The mitotic cells of different lymphatic cell subsets were identified with monoclonal anti-sera against T-helper-, T-suppressor-, and B-cells using the immunoperoxidase technic. The cells were counterstained with Giemsa for morphologic identification. After removal of Giemsa stain and peroxidase, the chromosomes were G-banded.
L
L
Teerenhovi
V
V
Wasenius
K
K
Franssila
M
M
Keinanen
S
S
Knuutila
D001399:Azure Stains; D002871:Chromosome Banding; D003584:Cytological Techniques; D007124:Immunoenzyme Techniques; D007621:Karyotyping; D008214:Lymphocytes / Q000145:classification*; D013194:Staining and Labeling
D001399:Azure Stains