Plasma and tissue-free amino acids in the developing chick.
The free amino acids of the plasma and pectoral muscle, liver and brain cytosolic S105 supernatant obtained from 2- and 3-week-old fasted chicks, were analyzed by HPLC after deproteinization by ultrafiltration. Protein-bound amino acids were analyzed in parallel studies. A clear relationship between the free and bound tissue amino acids was apparent. Only for the gluconeogenic amino acids a close correlation was evident between the plasma and the muscle free amino acids. A relatively steady composition of the free amino acids is established early in the tissues, but in the plasma it changes significantly with age.
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Laboratoire de Physiologie Métabolique et Nutrition, Université Pierre-et-Marie-Curie, Paris, France.
Pascaud
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D000375:Aging / Q000378:metabolism; D000596:Amino Acids / Q000097:blood / Q000378:metabolism*; D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D003600:Cytosol / Q000378:metabolism; D014462:Ultrafiltration
D000596:Amino Acids