Effects of analogues of substance P fragments on the MAO activity in rat brain.
The influence in vitro of analogues of Sp5-11 and SP6-11 substance P fragments on the activity of monoamine oxidase (MAO) in homogenates and crude mitochondrial fractions of rat brain was examined. The rat brain was divided into: I--cerebral cortex, II--hippocampus, III--midbrain, IV--thalamus with hypothalamus, V--cerebellum and VI--medulla oblongata. The obtained results proved that the analogues of SP fragments inhibit selectively the activity of the enzyme in the homogenates of cerebral cortex, hippocampus, midbrain and cerebellum. In the crude mitochondrial fractions the applied analogues of SP fragments caused a slight increase of the enzyme activity. The most significant changes in the activity of MAO were observed in hippocampus homogenate fraction.
E
E
Turska
L
L
Lachowicz
W
W
Koziołkiewicz
T
T
Wasiak
D000818:Animals; D001921:Brain / Q000201:enzymology*; D066298:In Vitro Techniques; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D008995:Monoamine Oxidase / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D010446:Peptide Fragments / Q000494:pharmacology*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D051381:Rats; D011919:Rats, Inbred Strains; D013373:Substance P / Q000494:pharmacology*
D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D013373:Substance P; C032292:substance P (6-11); C031661:substance P (5-11); D008995:Monoamine Oxidase; D011761:Pyrrolidonecarboxylic Acid