Hematopoietic factor production by a cell line (TC-1) derived from adherent murine marrow cells.
An adherent cell line, termed TC-1, has been isolated from long-term liquid culture of murine marrow cells by repeated exposure of the adherent cells to 0.1% trypsin. This is an alkaline phosphatase-positive cell line showing variable staining with acid phosphatase and alpha-naphthyl acetate esterase. On electron microscopy, the cells have moderate amounts of rough endoplasmic reticulum and variable numbers of polyribosomes. Some cells contain large clusters of laked glycogen particles. Intermediate junctions are present between some cells. Conditioned medium from this cell line produced from 384 to 638 units of CSF-1 per milliliter by radioimmunoassay and a CSF-1-dependent synergistic activity, which stimulates giant macrophage colony formation of marrow cells in soft agar. The conditioned medium also stimulates 3H-TdR incorporation by marrow cells in liquid culture and induces secondary adherent cell lines. The growth factor(s) produced by the TC-1 stromal cell line may be important in the regulation of early stages of hematopoietic differentiation. Two subclones, TC-1-C-11 and TC-1-C-3, have been isolated from passage 25 of the TC-1 cells by a penicylinder separation technique. The TC-1-C-11 is phenotypically like the parent TC-1 line and produces macrophage growth factors. The TC-1-C-3 grows as an epithelioid monolayer with visible junctions among adjacent cells under phase contrast microscopy. This subclone produces retrovirus and is capable of providing anchorage support for hematopoietic stem cells. The TC-1 cell line and its subclones may provide models for the control of early stem cell proliferation and differentiation.
Z X
ZX
Song
R K
RK
Shadduck
D J
DJ
Innes
A
A
Waheed
P J
PJ
Quesenberry
D000362:Agar; D000818:Animals; D001853:Bone Marrow / Q000378:metabolism / Q000528:radiation effects; D001854:Bone Marrow Cells; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000378:metabolism*; D003470:Culture Media / Q000378:metabolism; D006133:Growth Substances / Q000378:metabolism; D016298:Hematopoietic Cell Growth Factors; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000382:microbiology; D006651:Histocytochemistry; D008264:Macrophages / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D013194:Staining and Labeling; D013997:Time Factors
D003115:Colony-Stimulating Factors; D003470:Culture Media; D006133:Growth Substances; D016298:Hematopoietic Cell Growth Factors; D000362:Agar