Comparison of in vivo and in vitro methods for assessing the effects of phenobarbital on the hepatic drug-metabolizing enzyme system.
The effect of daily i.p. injections of 0, 1, 10 and 80 mg/kg phenobarbital for 1 week on the activity of the hepatic drug-metabolizing enzyme system was measured in the rat by a model substrate assay employing lindane (gamma-HCH) and by a battery of in vitro enzyme assays. Comparison of the dose-response curves of the in vivo and in vitro assays indicated that urinary metabolites of lindane provided a good index of phenobarbital-induced change in both phase I and phase II reactions.
Chadwick
R W
RW
Copeland
M F
MF
Carlson
G P
GP
Trela
B A
BA
Most
B M
BM
D000818:Animals; D002733:Chlorophenols / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D014453:Glucuronosyltransferase / Q000032:analysis; D005982:Glutathione Transferase / Q000032:analysis; D001556:Hexachlorocyclohexane / Q000378:metabolism; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344
D002733:Chlorophenols; D004364:Pharmaceutical Preparations; D001556:Hexachlorocyclohexane; D014453:Glucuronosyltransferase; D005982:Glutathione Transferase; D010634:Phenobarbital