Binding and immunological properties of a synthetic peptide corresponding to the phosphorylcholine-binding region of C-reactive protein.
A synthetic peptide corresponding to amino acid residues 47-63 of human C-reactive protein (CRP) was synthesized and evaluated for its ability to bind phosphorylcholine (PC) and to react with mAb specific for the PC-binding region of CRP. The PC-binding peptide displayed Ca2(+)-independent binding specific for PC and was able to compete against CRP for PC in the presence of Ca2+ ions. The synthetic peptide, like CRP, binds to the extracellular matrix protein fibronectin and the basement membrane protein laminin. The PC-binding peptide was recognized by those mAb generated against the intact CRP molecule that bind at, or near, the functional PC-binding region. In addition, several mAb to the T-15 idiotype present on mouse antibodies specific for PC, recognize an epitope(s) on the PC-binding peptide. Therefore, the 17 amino acid synthetic peptide shares both functional binding activity and antigenicity with the corresponding functional region within the CRP molecule.
Department of Microbiology, Ohio State University, Columbus.
S J
SJ
Swanson
R F
RF
Mortensen
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D001665:Binding Sites; D001667:Binding, Competitive; D002097:C-Reactive Protein / Q000302:isolation & purification / Q000378:metabolism*; D002794:Choline / Q000031:analogs & derivatives*; D006801:Humans; D007130:Immunoglobulin Idiotypes; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology / Q000378:metabolism*; D010767:Phosphorylcholine / Q000378:metabolism*; D012995:Solubility
D000911:Antibodies, Monoclonal; D007130:Immunoglobulin Idiotypes; D010455:Peptides; D010767:Phosphorylcholine; D002097:C-Reactive Protein; D002794:Choline