Biochemical consequences of follicle-stimulating hormone binding to testicular macrophages in culture.
The present studies demonstrate that testicular macrophages respond to follicle-stimulating hormone (FSH) by: 1) stimulating the rate of incorporation of amino acids into secreted proteins; 2) increasing the rate of incorporation of uridine into RNA; and 3) enhancing the accumulation of intracellular cyclic adenosine monophosphate (cAMP; which was potentiated by the addition of 1 mM 3-isobutyl-1-methylxanthine; MIX). In addition, dibutyryl cAMP (dbcAMP) enhanced the incorporation of amino acids into secreted proteins; however, this cAMP analog had no effect on the incorporation of uridine into RNA. Finally, it was demonstrated that testicular macrophages possess specific receptors with a high affinity for FSH.
J B
JB
Yee
J C
JC
Hutson
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D005640:Follicle Stimulating Hormone / Q000378:metabolism* / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D011956:Receptors, Cell Surface / Q000378:metabolism; D011962:Receptors, FSH; D013737:Testis / Q000166:cytology*; D014529:Uridine / Q000378:metabolism
D000596:Amino Acids; D011506:Proteins; D011956:Receptors, Cell Surface; D011962:Receptors, FSH; D012313:RNA; D003994:Bucladesine; D005640:Follicle Stimulating Hormone; D000242:Cyclic AMP; D014529:Uridine