Histidine-94 is the only important histidine residue in the melibiose permease of Escherichia coli.
Oligonucleotide-directed, site-specific mutagenesis has been utilized to modify the melB gene of Escherichia coli such that each of the seven His residues in the melibiose permease has been replaced with Arg. Replacement of His-213, His-442, or His-456 has no significant effect on permease activity, while permease with Arg in place of His-198, His-318, or His-357 retains more than 70% of wild-type activity. In striking contrast, replacement of His-94 with Arg causes a complete loss of sugar binding and transport, although the cells contain a normal complement of permease molecules. Thus, as shown previously with lac permease, only a single His residue is important for activity, but, in the case of mel permease, the critical His residue is present in the 3rd putative transmembrane helix rather than the 10th.
Pourcher
T
T
Laboratoire J. Maetz, Département de Biologie du Commissariat à l'Energie Atomique, Villefranche sur mer, France.
Sarkar
H K
HK
Bassilana
M
M
Kaback
H R
HR
Leblanc
G
G
D001120:Arginine; D001483:Base Sequence; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D005798:Genes, Bacterial; D006639:Histidine; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D015345:Oligonucleotide Probes; D010957:Plasmids; D011487:Protein Conformation; D027981:Symporters
D026901:Membrane Transport Proteins; D015345:Oligonucleotide Probes; D027981:Symporters; D006639:Histidine; C021548:melibiose permease; D001120:Arginine