A retinoic acid responsive gene, MK, produces a secreted protein with heparin binding activity.
MK is a gene whose expression increases transiently during retinoic acid-induced differentiation of embryonal carcinoma cells. MK polypeptide was secreted by differentiating HM-1 embryonal carcinoma cells and by L-cells transfected with an MK cDNA under the control of the beta-actin promoter and Rous sarcoma virus enhancer. MK polypeptide was found to have heparin binding activity. Conditioned medium of the transfected L-cells promoted growth of PC-12 pheochromocytoma cells. These findings support the view that MK polypeptide is a secreted factor involved in regulation of growth and differentiation.
Department of Biochemistry, Faculty of Medicine, Kagoshima University, Japan.
M
M
Tomomura
K
K
Kadomatsu
M
M
Nakamoto
H
H
Muramatsu
H
H
Kondoh
K
K
Imagawa
T
T
Muramatsu
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D002846:Chromatography, Affinity; D015870:Gene Expression / Q000187:drug effects*; D006493:Heparin / Q000378:metabolism*; D007700:Kinetics; D007739:L Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D013724:Teratoma; D014162:Transfection; D014212:Tretinoin / Q000494:pharmacology*
D002352:Carrier Proteins; D014212:Tretinoin; D006493:Heparin