Analysis of 6-phosphogluconate dehydrogenase isozymes by polyacrylamide gel isoelectric focusing.
Three common phenotypes of 6-phosphogluconate dehydrogenase (6-PGD) were identified by enzyme visualization following isoelectric focusing in photochemically polymerized polyacrylamide gels whereas in chemically polymerized gels no activity could be detected. Gene frequencies calculated from 429 individuals were 0.915 and 0.085 for 6-PGD*A and 6-PGD*C, respectively, similar to the gene frequencies of Japanese samples previously reported.
Akiyama
K
K
Department of Legal Medicine, Tokyo Women's Medical College, Japan.
D000180:Acrylic Resins; D000665:Ampholyte Mixtures; D005787:Gene Frequency; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods; D007527:Isoenzymes / Q000097:blood* / Q000235:genetics; D007564:Japan; D010641:Phenotype; D010734:Phosphogluconate Dehydrogenase / Q000097:blood* / Q000235:genetics
D000180:Acrylic Resins; D000665:Ampholyte Mixtures; D007527:Isoenzymes; C016680:polyacrylamide gels; D010734:Phosphogluconate Dehydrogenase