Actin participates in the structure of liver intermediate filaments.
A dominating protein fraction (p45) having molecular weight of 45000 and pI 5.45 was found in the intermediate filaments pellet obtained from rat liver besides the present cytokeratins. Peptide mapping and radioimmunological assays with antibodies against this protein and muscle actin proved that the p45 protein belongs to the actin group. Immunoelectron microscopy revealed that this protein is located on the liver intermediate filaments. By melting of the cytokeratin complexes in urea it was established that p45 protein is complexed with the low molecular weight cytokeratin.
R G
RG
Pankov
A A
AA
Uschewa
B T
BT
Tasheva
P T
PT
Petrov
G G
GG
Markov
D000199:Actins / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D003599:Cytoskeleton / Q000648:ultrastructure*; D007382:Intermediate Filaments / Q000378:metabolism / Q000648:ultrastructure*; D007633:Keratins / Q000302:isolation & purification; D008099:Liver / Q000378:metabolism / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D011863:Radioimmunoassay; D051381:Rats
D000199:Actins; D010446:Peptide Fragments; D007633:Keratins