Purification of neurocatin, a neuroregulatory factor from brain.
A neuroregulatory factor (neurocatin) has been isolated from bovine brain. Neurocatin is a powerful affector of catecholamine metabolism in synaptosomes isolated from rat brain, causing increased formation of norepinephrine (NE) and decreased formation of 3,4-dihydroxyphenylacetic acid (DOPAC). The ratio NE/DOPAC in synaptosomes has been used to measure the neurocatin content of samples during isolation. Neurocatin has now been purified to a single chromatographic peak by high-resolution HPLC and a preliminary amino acid content measured. It appears to be a small peptide with a molecular weight in the range of 2,000-2,500 Da.
Yee
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DK
Department of Biochemistry and Biophysics, Medical School, University of Pennsylvania, Philadelphia 19104.
Pastuszko
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P
Pastuszko
A
A
Wilson
D F
DF
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D002417:Cattle; D002850:Chromatography, Gel; D008297:Male; D009419:Nerve Tissue Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D009479:Neuropeptides / Q000302:isolation & purification*; D009638:Norepinephrine / Q000096:biosynthesis*; D010648:Phenylacetates / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*
D000596:Amino Acids; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D010648:Phenylacetates; D015102:3,4-Dihydroxyphenylacetic Acid; D009638:Norepinephrine