An immunoblot technique for identification of allergens of the Australian paralysis tick Ixodes holocyclus.
The blocking conditions appropriate for Western blot identification of allergens of the Australian paralysis tick, Ixodes holocyclus, have been investigated. The results suggest that human serum is a potent blocking agent which may have wide application in the immunodetection of antigens.
CSIRO, Division of Tropical Animal Production, Long Pocket Laboratories, Indooroopilly, Queensland, Australia.
M
M
Gauci
B F
BF
Stone
Y H
YH
Thong
D000485:Allergens / Q000032:analysis*; D000818:Animals; D005260:Female; D006863:Hydrogen-Ion Concentration; D015151:Immunoblotting / Q000379:methods*; D007124:Immunoenzyme Techniques; D008970:Molecular Weight; D013987:Ticks / Q000276:immunology*
D000485:Allergens