Distribution of negative charges of rat brain synaptosomes established by means of protamine-ferritin conjugate.
The negative electric charges on the synaptosomes from rat cerebral cortex were studied by means of protamine-ferritin conjugate. The synaptic vesicles in some synaptosomes were heavily labelled with the positively charged conjugate. The synaptosomal membranes including presynaptic and postsynaptic membranes were also stained but in a lesser degree. It was established that the major dense line and the intraperiod line of myelin contaminants were labelled, too.
K
K
Ichev
W
W
Ovtscharoff
U
U
Pfüller
P
P
Bergmann
H
H
Franz
L
L
Venkov
D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism* / Q000648:ultrastructure; D004563:Electrochemistry; D005293:Ferritins; D006651:Histocytochemistry; D008854:Microscopy, Electron; D011479:Protamines; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling; D013574:Synaptosomes / Q000378:metabolism* / Q000648:ultrastructure
D011479:Protamines; C049117:protamine-ferritin conjugate; D005293:Ferritins