Purification and partial characterization of a monoclonal "second order" suppressor factor specific for L-glutamic acid60-L-alanine30-L-tyrosine10.
A GAT-specific "second order" suppressor T cell factor (TsF2) from the hybridoma 762 B3.7 has been purified and biochemically characterized. The protein has a m.w. of approximately 66,000, an isoelectric point of 6.8 to 6.9, and elutes from a reversed phase HPLC column in two peaks, one in 55% acetonitrile, the other in 70% propanol. Amino acid analysis of both forms gave similar molar ratios, suggesting that the two forms are closely related and may differ mainly in the degree of posttranslational modification. SDS-PAGE electrophoresis under reducing conditions gave two chains of the apparent m.w. of 42,000 and 35,000.
C W
CW
Turck
J A
JA
Kapp
D R
DR
Webb
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D006825:Hybridomas / Q000378:metabolism*; D007525:Isoelectric Focusing; D051379:Mice; D008970:Molecular Weight; D010455:Peptides / Q000276:immunology*; D011108:Polymers; D011233:Precipitin Tests; D013491:Suppressor Factors, Immunologic / Q000032:analysis / Q000302:isolation & purification* / Q000502:physiology; D013601:T-Lymphocytes / Q000378:metabolism*
D000939:Epitopes; D010455:Peptides; D011108:Polymers; D013491:Suppressor Factors, Immunologic; C020749:GAT