Low light level in vitro monitoring of cellular and antigen-antibody reactions using a photon detection camera system--new perspectives for clinical diagnosis and research.
This article briefly describes the use of a photon counting system (ARGUS-100) in the detection of low levels of light. The ARGUS-100 was used in determining ATP in cell sections from tumor tissues and in measuring a luminescence-enhanced immunoluminometric assay, using ferritin as the analyte, based on the luminol-peroxide-4-iodophenol reaction with peroxidase as the enzyme. The aim is not so much the presentation of data, but rather to show the potentials of the photon counting camera in increasing our knowledge of the cellular and subcellular levels, as well as lowering the detection limits in already sensitive systems, such as immunoassays.
Klinik für Innere Medizin, Medizinische Universität zu Lübeck.
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Wiedemann
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Müller-Klieser
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Walenta
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Schleinkofer
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Wood
D000230:Adenocarcinoma; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D000937:Antigen-Antibody Reactions / Q000276:immunology*; D001786:Blood Glucose / Q000378:metabolism*; D002460:Cell Line; D003199:Computer Systems; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation*; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008163:Luminescent Measurements; D008838:Microcomputers; D051381:Rats; D012208:Rhabdomyosarcoma; D012815:Signal Processing, Computer-Assisted; D014407:Tumor Cells, Cultured / Q000473:pathology*; D014741:Video Recording / Q000295:instrumentation*
D001786:Blood Glucose; D007773:Lactates; D019344:Lactic Acid; D000255:Adenosine Triphosphate