Porphyrin-induced photodynamic cross-linking of hepatic heme-binding proteins.
Three types of hepatic proteins, a heme-binding Z protein, a mixture of the glutathione S-transferases and a cytochrome P450 isozyme, were shown to be susceptible to photodynamic cross-linking and loss in antigenicity by naturally occurring porphyrins. At 50 microM, uroporphyrin caused the most and protoporphyrin the least photodecomposition. Hemopexin, a specific serum heme carrier, was photodecomposed but no cross-linking was detected. Heme and scavengers of singlet oxygen partially prevented protein photodecomposition.
S H
SH
Vincent
B
B
Holeman
B C
BC
Cully
U
U
Muller-Eberhard
D000818:Animals; D002352:Carrier Proteins / Q000276:immunology / Q000528:radiation effects*; D003306:Coproporphyrins / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000528:radiation effects; D000939:Epitopes / Q000528:radiation effects; D005982:Glutathione Transferase / Q000528:radiation effects; D006418:Heme / Q000494:pharmacology; D000081062:Heme-Binding Proteins; D006420:Hemeproteins; D006466:Hemopexin / Q000528:radiation effects; D006639:Histidine / Q000494:pharmacology; D007122:Immunoelectrophoresis; D008027:Light; D008099:Liver / Q000032:analysis*; D010777:Photochemistry; D011166:Porphyrins / Q000494:pharmacology*; D011524:Protoporphyrins / Q000494:pharmacology; D051381:Rats; D013696:Temperature; D013997:Time Factors; D014466:Ultraviolet Rays; D014578:Uroporphyrins / Q000494:pharmacology
D002352:Carrier Proteins; D003306:Coproporphyrins; D000939:Epitopes; D000081062:Heme-Binding Proteins; D006420:Hemeproteins; D011166:Porphyrins; D011524:Protoporphyrins; D014578:Uroporphyrins; D006418:Heme; D006639:Histidine; D006466:Hemopexin; D003577:Cytochrome P-450 Enzyme System; D005982:Glutathione Transferase