Determination of glycated hemoglobins of cadaveric blood: a study involving the use of affinity chromatography with m-amino phenyl boronic acid.
For a postmortem diagnosis of diabetes mellitus (DM), the applicability of achieving a determination by examining the glycated hemoglobins (GHb) is discussed. Experimental studies by the use of affinity chromatography with m-amino phenyl boronic acid-agarose revealed that the GHb values could be determined stably and could be reproduced even for some postmortem models of both non-diabetic and diabetic human blood. A subsequent survey of autopsy cases with a proper past DM medical history has suggested that this method is useful, especially when used with customary medicolegal examinations conducted for DM.
Kuroda
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Department of Legal Medicine, School of Medicine, Keio University, Tokyo, Japan.
D000293:Adolescent; D000328:Adult; D000368:Aged; D001897:Boronic Acids; D002102:Cadaver; D002846:Chromatography, Affinity / Q000379:methods; D003920:Diabetes Mellitus / Q000175:diagnosis*; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis*; D006801:Humans; D008297:Male; D008875:Middle Aged
D001897:Boronic Acids; D006442:Glycated Hemoglobin; C028592:3-aminobenzeneboronic acid