Circulating cortisol & related steroids following ketoconazole challenge in Cushing's disease.
Circulating levels of cortisol, 17 alpha hydroxy progesterone and delta 4 androstenedione were analysed by specific radioimmunoassays in 11 patients with Cushing's disease before and at 2, 4, 6, 12 and 24# h following an oral dose of 400 mg ketoconazole. A significant fall in cortisol (26 - 79.6%, 59.8 +/- 18.7 SEM), delta 4 androstenedione (21.5 - 63.3%, 47.8 +/- 4.87) with a concomitant rise in 17 alpha hydroxy progesterone (113 - 218%, 116 +/- 11.43) were noted, suggesting inhibition of 17, 20 desmolase and 11 beta-hydroxylase enzymes in cortisol biosynthetic pathway.
Dash
R J
RJ
Department of Endocrinology, Postgraduate Institute of Medical Education & Research, Chandigarh.
Khandekar
S
S
Lata
V
V
Bhansali
A
A
D019326:17-alpha-Hydroxyprogesterone; D000446:Aldehyde-Lyases / Q000037:antagonists & inhibitors; D000735:Androstenedione / Q000097:blood; D003480:Cushing Syndrome / Q000097:blood / Q000188:drug therapy*; D065607:Cytochrome P-450 Enzyme Inhibitors; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D006908:Hydroxyprogesterones / Q000097:blood; D007654:Ketoconazole / Q000494:pharmacology*; D008297:Male; D013252:Steroid 11-beta-Hydroxylase / Q000037:antagonists & inhibitors; D013254:Steroid 17-alpha-Hydroxylase
D065607:Cytochrome P-450 Enzyme Inhibitors; D006908:Hydroxyprogesterones; D000735:Androstenedione; D019326:17-alpha-Hydroxyprogesterone; D013254:Steroid 17-alpha-Hydroxylase; D013252:Steroid 11-beta-Hydroxylase; D000446:Aldehyde-Lyases; D007654:Ketoconazole; D006854:Hydrocortisone