T cell clones to two major T cell epitopes of myoglobin: effect of I-A/I-E restriction on epitope dominance.
The murine T cell response to sperm whale myoglobin was analyzed for polyclonal and monoclonal T cells. For polyclonal T cells, the immunodominant epitope included residue Glu 109 when the antigen-presenting cells expressed I-Ad, whereas a Lys 140-containing epitope was immunodominant when the antigen-presenting cells expressed I-Ed only. Monoclonal T cells specific for each epitope were derived from a polyclonal line. T cell clones specific for the Glu 109 epitope were restricted to I-Ad, whereas the clones specific for the Lys 140 epitope were restricted to I-Ed. Thus, for an antigen that can be presented in association with either I-Ad or I-Ed, the immunodominance of particular epitopes depends strongly on the restriction element used. The immunodominance of each epitope-Ia combination may be due to a limited repertoire of T cells or selective presentation of epitope and Ia by accessory cells.
Berkower
I
I
Kawamura
H
H
Matis
L A
LA
Berzofsky
J A
JA
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D002999:Clone Cells / Q000145:classification / Q000276:immunology; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D005260:Female; D005799:Genes, Dominant; D005802:Genes, MHC Class II; D005816:Genetic Complementation Test; D006649:Histocompatibility Antigens / Q000235:genetics; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009211:Myoglobin / Q000276:immunology*; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*; D014907:Whales / Q000276:immunology
D000939:Epitopes; D006649:Histocompatibility Antigens; D000949:Histocompatibility Antigens Class II; C024138:I-E-antigen; D009211:Myoglobin