[Heterogeneity of binding proteins of proteoglycan aggregates from human hyaline cartilage under normal conditions and in systemic bone dysplasias].
Using SDS electrophoresis and subsequent densitometry, the link proteins (LP) of proteoglycan aggregates of the knee joint hyaline cartilage, rib and/or the iliac crest cartilage were investigated. Both the control and experimental samples (n = 9 and n = 16, respectively) contained three LP with Mr 48.0 (LP-1), 44.0 (LP-2) and 41.5 KD (LP-3); however, their ratio varied within very broad limits. Low molecular weight forms of LP were also observed in the infundibulum-like deformation of the thorax. The considerable decrease of LP-3 and the elevated content of LP-2 were observed in lethal osteochondrodysplasias, which probably reflects the genetically determined disorder of limb morphogenesis, eventually resulting in the maintenance of embryonic ratio of LP. Almost all the preparations contained a protein with Mr 52 KD that was previously unknown for the LP system. The content of this protein was the highest in the exostose cartilage and in newborns. Possible mechanisms of LP heterogeneity and the significance of this parameter for the regulation of chondrogenesis and realization of certain physical properties of cartilages from different parts of the skeleton are discussed.
S P
SP
Feshchenko
K D
KD
Krasnopol'skaia
S S
SS
Shishkin
I R
IR
Erber
D001848:Bone Diseases, Developmental / Q000378:metabolism*; D002352:Carrier Proteins / Q000032:analysis*; D002356:Cartilage / Q000378:metabolism*; D002358:Cartilage, Articular / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006818:Hyalin / Q000378:metabolism*; D007231:Infant, Newborn; D008970:Molecular Weight; D011509:Proteoglycans / Q000032:analysis*
D002352:Carrier Proteins; D011509:Proteoglycans
Geterogennost' sviazyvaiushchikh belkov proteoglikanovykh agregatov gialinovogo khriashcha cheloveka v norme i pri sistemnykh kostnykh displaziiakh.