[Somatic antigens of the Brucella genus. The structure of the O-specific polysaccharide chain of Brucella melitensis lipopolysaccharide].
The phenol-phase soluble antigenic lipopolysaccharide was isolated from Brucella melitensis, strain 565, by the routine phenol/water procedure followed by chromatography on Sepharose 4B. After mild acid hydrolysis and chromatography on Sephadex G-50, the lipopolysaccharide yielded a linear O-specific polysaccharide built up from 1,2-linked 4,6-dideoxy-4-formamido-alpha-D-mannopyranosyl units. The structure of the polysaccharide was deduced mainly from the nuclear magnetic resonance and methylation analyses. The phenol-soluble lipopolysaccharide, isolated from commercial vaccine strain B. abortus 19-BA, on mild hydrolysis afforded material, 13C and 1H-NMR spectra of which were identical to those of the O-specific polysaccharide from B. melitensis 565.
V L
VL
L'vov
V E
VE
Malikov
A S
AS
Shashkov
E A
EA
Dranovskaia
B A
BA
Dmitriev
D000942:Antigens, Bacterial / Q000032:analysis*; D002002:Brucella / Q000145:classification*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D008070:Lipopolysaccharides / Q000032:analysis*; D019081:O Antigens; D012703:Serotyping
D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D019081:O Antigens
Somaticheskie antigeny roda Brucella. Stroenie O-spetsificheskoĭ polisakharidnoĭ tsepi lipopolisakharida Brucella melitensis.