Characterization of calcium and sodium currents in porcine pars intermedia cells.
The whole-cell configuration of the patch-clamp technique was applied to porcine pars intermedia cells. A tetrodotoxin-sensitive sodium current was recorded. Three types of calcium current were observed. Depolarizations from a holding potential of 100 mV elicited a transient current (ICaT), whereas depolarizations from a holding potential of -40 mV evoked a sustained current (ICaS). A third current (ICaN; N for neither) was activated by strong depolarization to +10 or +20 mV from holding potentials of - 100 mV. Increasing internal Ca2+ significantly reduced the amplitude of ICaS.
Taleb
O
O
Trouslard
J
J
Demeneix
B A
BA
Feltz
P
P
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D004056:Differential Threshold; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D008564:Membrane Potentials; D010902:Pituitary Gland / Q000166:cytology / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D013552:Swine; D013779:Tetrodotoxin / Q000494:pharmacology
D007473:Ion Channels; D013779:Tetrodotoxin; D012964:Sodium; D002118:Calcium