Large scale preparation of homogeneous bacteriorhodopsin.
Homogeneous bacteriorhodopsin was obtained preparatively (100 mg batches) from purple membrane of Halobacterium halobium cells. The homogeneity of the protein was considerably affected by variations in the growth conditions of the bacteria. Fully matured bacteriorhodopsin having a blocked N-terminus and a homogeneous C-terminus, was reproducibly obtained when cells were grown in a sufficiently aerated medium.
Center for Macromolecular Crystallography, University of Alabama, Birmingham, 35294.
B
B
Lorber
L J
LJ
DeLucas
D000595:Amino Acid Sequence; D001431:Bacteriological Techniques; D001436:Bacteriorhodopsins / Q000302:isolation & purification*; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D006217:Halobacterium / Q000032:analysis* / Q000254:growth & development; D007525:Isoelectric Focusing; D008027:Light; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010100:Oxygen
D001436:Bacteriorhodopsins; D010100:Oxygen