Stimulation by the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine of rat hepatic polyamine biosynthesis in vivo.
Intraperitoneal injection of the phosphodiesterase inhibitor, 3-isobutyl-1-methylxanthine (IBMX), resulted in a rapid and transient induction of rat hepatic ornithine decarboxylase (ODC) activity. Maximal activity was found about 5 hr after application. The levels of putrescine and spermidine increased accordingly, reaching a maximum at 7 and 12 hr following injection, respectively, while the concentration of spermine remained almost constant. The implications of these findings are discussed in relation to the mechanism of induction of ornithine decarboxylase and concomitant polyamine biosynthesis.
L A
LA
van Rooijen
H J
HJ
Derks
R
R
van Wijk
A
A
Bisschop
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology*; D000818:Animals; D005260:Female; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D065108:Ornithine Decarboxylase Inhibitors; D011073:Polyamines / Q000096:biosynthesis*; D011700:Putrescine / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D013095:Spermidine / Q000096:biosynthesis; D013096:Spermine / Q000096:biosynthesis; D013806:Theophylline / Q000031:analogs & derivatives*
D065108:Ornithine Decarboxylase Inhibitors; D011073:Polyamines; D013096:Spermine; D013806:Theophylline; D015056:1-Methyl-3-isobutylxanthine; D013095:Spermidine; D011700:Putrescine