Demonstration and distribution of kassinin-like material (substance K) in the rat central nervous system.
Antiserum was raised against kassinin in rabbits. Cross-reactivity with other tachykinins was determined; these included substance K (100%) and substance P (0.1%). Peptides extracted from rat brain and synthetic tachykinins were chromatographed by reverse-phase HPLC. The major peak of kassinin-like material eluted at a time different from that of synthetic kassinin, eledoisin, physalaemin, neurokinin beta, and substance P but coeluted with substance K. Measurement of kassinin-like material in macrodissected and microdissected brain regions indicated that the distribution of kassinin-like material was similar to that of substance P.
Shults
C W
CW
Yajima
H
H
Gullner
H G
HG
Chase
T N
TN
O'Donohue
T L
TL
D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000502:physiology*; D002851:Chromatography, High Pressure Liquid; D007106:Immune Sera / Q000276:immunology; D015286:Kassinin; D008297:Male; D009419:Nerve Tissue Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000502:physiology; D015288:Neurokinin A; D009842:Oligopeptides / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D013373:Substance P / Q000276:immunology
D007106:Immune Sera; D009419:Nerve Tissue Proteins; D009842:Oligopeptides; D013373:Substance P; D015286:Kassinin; D015288:Neurokinin A