Two apparently nonrepeated epitopes on gametes of Plasmodium falciparum are targets of transmission-blocking antibodies.
One-site and two-site immunoradiometric assays have been developed against an antigen on gametocytes of Plasmodium falciparum, using monoclonal antibodies (Mabs) which block transmission of the parasites to mosquitoes. Three such Mabs have been studied, each of which immunoprecipitates a complex of three gamete surface proteins of apparent Mr 260,000, 59,000, and 53,000 from Triton X-100 extracts of the parasites. The assays showed that the Mabs recognized one or the other of two distinct, nonrepeated epitopes on the target antigen(s). In the one-site assay certain combinations of two Mabs interacted at appropriate concentrations to enhance binding of the Mabs to the antigen. The same combinations of Mabs synergize to suppress infectivity of gametocytes to mosquitoes.
R
R
Carter
G
G
Bushell
A
A
Saul
P M
PM
Graves
C
C
Kidson
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology*; D001667:Binding, Competitive; D000939:Epitopes; D046911:Macromolecular Substances; D010963:Plasmodium falciparum / Q000166:cytology / Q000276:immunology*; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D000939:Epitopes; D046911:Macromolecular Substances