Antigenic determinants of pregnancy-associated alpha 2-glycoprotein and alpha 2-macroglobulin defined by poly- and monoclonal antibodies.
Human alpha 2-macroglobulin and pregnancy-associated alpha 2-glycoprotein (PA alpha 2G) share several physicochemical characteristics. By the use of unabsorbed or absorbed polyclonal antibodies to these antigens, the existence of common epitopes in these molecules were demonstrated in crossed immunoelectrophoresis. Two monoclonal antibodies out of 9 raised against purified PA alpha 2G were demonstrated to react with both antigens, indicating close immunochemical relatedness between these macroglobulins. The findings might have functional implications.
L
L
Carlsson
J
J
Folkersen
T
T
Stigbrand
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D000939:Epitopes; D005260:Female; D006801:Humans; D005779:Immunodiffusion; D007123:Immunoelectrophoresis, Two-Dimensional; D008253:Macaca mulatta; D008297:Male; D051379:Mice; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000276:immunology*; D011817:Rabbits; D051381:Rats; D000511:alpha-Macroglobulins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D011257:Pregnancy Proteins; D000511:alpha-Macroglobulins