Esterases in Folsomia candida (Collembola: Isotomidae). Characterization of enzymes among parthenogenic strains.
Esterase enzymes from four strains of Folsomia candida were investigated using polyacrylamide gel electrophoresis. Up to 12 bands of enzymatic activity were present in each strain. Esterase bands were classified as choline esterases or as one of two groups of carboxyl esterases, based on mobility, on substrate specificity and on activity remaining after inhibition by class-specific chemicals. One strain-specific choline esterase was discovered which resisted the effects of many organophosphate inhibitors. Organophosphate inhibitor concentrations had to be 10 to 100 times greater to reduce the staining activity of this resistant choline esterase to the level of comparable esterases in other strains.
Grimnes
K A
KA
D000818:Animals; D002219:Carbamates; D004591:Electrophoresis, Polyacrylamide Gel; D004950:Esterases / Q000378:metabolism*; D006868:Hydrolysis; D007313:Insecta / Q000201:enzymology* / Q000502:physiology; D007306:Insecticides / Q000494:pharmacology; D009943:Organophosphorus Compounds; D010312:Parthenogenesis; D013194:Staining and Labeling
D002219:Carbamates; D007306:Insecticides; D009943:Organophosphorus Compounds; D004950:Esterases