[Effect of chronic exposure to nitrogen dioxide on biogenic amine levels in the brain of guinea pigs].
The experiment was carried out on male guinea pigs exposed to NO2 at a constant concentration of 0.1 ppm 24 h daily for 1, 2.5 and 6 months, or NO2 concentration was increased to 0.5 ppm twice daily, between 7:00 and 8:00 AM and 4:00 and 5:00 PM. After the exposure to NO2 was over the animals were killed by decapitation. The cranium was opened and the brain removed, and afterwards at 0 degrees C the left hemisphere was dissected into discrete areas which were then frozen on dry ice and weighed. The tissues were kept for 2-3 days at -20 degrees C, and afterwards the content of noradrenaline, 5-hydroxytryptamine and 5-hydroxyindole-acetic acid was estimated spectrofluorometrically in examined parts of the brain (thalamus--hypothalamus pons + medulla oblongata hippocampus, cortex frontalis). It was shown that the applied NO2 concentrations brought about slight and nonspecific alterations in the content of the investigated amines in guinea pigs' brain.
W
W
Felińska
R
R
Szkilnik
B
B
Stanosek
M
M
Kamiński
K
K
Ludyga
R
R
Brus
D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D001679:Biogenic Amines / Q000378:metabolism*; D001923:Brain Chemistry / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D008297:Male; D009585:Nitrogen Dioxide / Q000633:toxicity*; D009638:Norepinephrine / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D013997:Time Factors
D000393:Air Pollutants; D001679:Biogenic Amines; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D009585:Nitrogen Dioxide; D009638:Norepinephrine
Wpływ przewlekłej ekspozycji dwutlenku azotu na zawartość amin biogennych w mózgu świnek morskich.