[Artificial peptide and carbohydrate antigens. Immobilization of haptens and adjuvant (MDP) on polyacrylamide].
To study the influence of the polyacrylamide carrier on immunogenic properties of the peptide and oligosaccharide haptens, we have prepared artificial antigens by conjugation of a synthetic hexapeptide (homologous to the fragment 95-100 of the murine H-2Db antigen heavy chain) or of an oligosaccharide (antigenic determinant of human blood groops, Lea) with polyacrylamide. In some cases the conjugates containing also a synthetic glycopeptide adjuvant, N-acetylmuramoyl-L-alanyl-D-isoglutamine (MDP), were used. Antisera against haptens were obtained by immunization of BALB/c mice with corresponding conjugates. By the enzyme-linked immunosorbent assay it was shown that these antisera had a high binding titer (up to 10 000) to corresponding hapten, and MDP immobilized on the same carrier as hapten possessed a considerable immunostimulating activity. Thus, usefulness of polyacrylamide for preparation of immunogenic artificial molecules carrying peptide and oligosaccharide haptens was demonstrated.
V V
VV
Iurovskiĭ
N V
NV
Bovin
N G
NG
Safonova
R G
RG
Vasilov
A Ia
AIa
Khorlin
D000119:Acetylmuramyl-Alanyl-Isoglutamine; D000180:Acrylic Resins; D000818:Animals; D002850:Chromatography, Gel; D000939:Epitopes / Q000032:analysis*; D006183:H-2 Antigens / Q000276:immunology*; D006241:Haptens; D006801:Humans; D007124:Immunoenzyme Techniques; D007983:Lewis Blood Group Antigens / Q000276:immunology*; D051379:Mice; D009844:Oligosaccharides / Q000276:immunology*; D000099166:Polyacrylamides
D000119:Acetylmuramyl-Alanyl-Isoglutamine; D000180:Acrylic Resins; D000939:Epitopes; D006183:H-2 Antigens; D006241:Haptens; D007983:Lewis Blood Group Antigens; D009844:Oligosaccharides; D000099166:Polyacrylamides
Iskusstvennye peptidnye i uglevodnye antigeny. Immobilizatsiia gaptenov i ad''iuvanta (MDP) na poliakrilamide.