Differential response of muscle and gastric histidine decarboxylase to compound 48/80 and dietary calcium.
Previous work from this laboratory had indicated that in vivo, histidine decarboxylase (HDC) activity was stimulated by compound 48/80 in rat leg muscle, and that high dietary calcium had a stimulating effect on gastric HDC activity. In the present investigations the 48/80 effect was also observed in vitro in leg muscle extracts from rats, chicks, and guinea pigs. Compound 48/80 had no effect in vitro on histamine metabolism of gastric tissue homogenates in any of the animal species studied. A dietary effect of high calcium intake was noted in rat gastric tissue but not in rat leg muscle. In vitro addition of 48/80 and/or calcium had no stimulatory effect on bacterial HDC or on muscle carnosinase activity. These findings, in conjunction with a comparison of stomach and leg muscle mast cell populations, confirm an HDC stimulatory role for 48/80 in muscle, in addition to its histamine-releasing function from mast cells.
S M
SM
Greene
H
H
Fisher
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D002136:Calcium, Dietary / Q000494:pharmacology*; D002262:Carboxy-Lyases / Q000032:analysis*; D002645:Chickens; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology; D006168:Guinea Pigs; D006636:Histamine Release / Q000187:drug effects; D006640:Histidine Decarboxylase / Q000032:analysis*; D007527:Isoenzymes / Q000032:analysis*; D008297:Male; D051379:Mice; D009132:Muscles / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D013045:Species Specificity; D013270:Stomach / Q000187:drug effects* / Q000201:enzymology; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology*
D001426:Bacterial Proteins; D002136:Calcium, Dietary; D007527:Isoenzymes; D003189:p-Methoxy-N-methylphenethylamine; D002262:Carboxy-Lyases; D006640:Histidine Decarboxylase