Immunogold labeling of metaphase cells.
A method is presented for the phenotypic identification of metaphase cells stained for chromosome aberration and SCE analysis. The cells are labeled in suspension with antibodies conjugated with colloidal gold, and then chromosome preparations are made using a cytocentrifuge. A silver development (IGSS) procedure is used to enhance the gold labeling for light microscopy. A variety of fixatives may be employed, permitting various cytogenetic and cytochemical staining procedures to be used.
P E
PE
Perry
E J
EJ
Thomson
D000906:Antibodies; D002869:Chromosome Aberrations; D002877:Chromosomes, Human / Q000648:ultrastructure*; D005404:Fixatives; D006046:Gold; D006801:Humans; D007158:Immunologic Techniques; D007621:Karyotyping / Q000379:methods*; D007773:Lactates; D019344:Lactic Acid; D008214:Lymphocytes / Q000648:ultrastructure; D008677:Metaphase; D012854:Sister Chromatid Exchange; D013048:Specimen Handling; D013194:Staining and Labeling
D000906:Antibodies; D005404:Fixatives; D007773:Lactates; D019344:Lactic Acid; D006046:Gold