Characterization of highly immunogenic p66/p51 as the reverse transcriptase of HTLV-III/LAV.
Approximately 80 percent of all human sera that react with antigens of HTLV-III, the etiologic agent of the acquired immune deficiency syndrome (AIDS), recognize protein bands at 66 and 51 kilodaltons. A mouse hybridoma was produced that was specific to these proteins. Repeated cloning of the hybridoma did not separate the two reactivities. The p66/p51 was purified from HTLV-III lysates by immunoaffinity chromatography and subjected to NH2-terminal Edman degradation. Single amino acid residues were obtained in 17 successive degradation cycles. The sequence determined was a perfect translation of the nucleotide sequence of a portion of the HTLV-III pol gene. The purified p66/51 had reverse transcriptase activity and the monoclonal immunoglobulin G specifically removed the enzyme activity from crude viral extract as well as purified enzyme.
di Marzo Veronese
F
F
Copeland
T D
TD
DeVico
A L
AL
Rahman
R
R
Oroszlan
S
S
Gallo
R C
RC
Sarngadharan
M G
MG
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D001483:Base Sequence; D002846:Chromatography, Affinity; D017977:Deltaretrovirus / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D005814:Genes, Viral; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012194:RNA-Directed DNA Polymerase / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D012194:RNA-Directed DNA Polymerase