A novel leukemia T-cell line (PF-382) with phenotypic and functional features of suppressor lymphocytes.
A human leukemia T-cell line (PF-382) spontaneously derived from the pleural effusion of a child with T-cell acute lymphoblastic leukemia is described. The cell line, which has been maintained in culture for over 10 months, has a modal number of 46 chromosomes and is characterized by a chromosomal abnormality, present in most of the cells, consisting of a translocation between chromosome X and chromosome 15 (46X,Xq-,15p+). The cells are not recognized by the OKT3 and OKT11 monoclonal antibodies (MoAb), nor do they form rosettes with sheep erythrocytes. By contrast, they react with the OKT6, Leu-1, and Leu-9 MoAb, which detect early T-lymphocytes, and express the more mature OKT8 antigen. The presence of the OKT8 marker is associated with suppressor activity on the pokeweed mitogen-induced proliferation and differentiation of normal B-cells, both by the PF-382 cells and by their supernatant. However, no cytotoxic activity against natural killer (NK)-sensitive target cells (K562) was found, indicating that the proliferating cells do not correspond to the subset of NK cells expressing the OKT8 antigen. Furthermore, the cells are incapable of both spontaneous and mitogen-induced interleukin-2 and interferon production. The ability of the PF-382 cell line to release a soluble factor(s) capable of modulating the differentiation of the B-cell compartment suggests that this new cell line represents a valuable model for the investigation of the interrelationships between T-cell subsets and other hematopoietic cell lineages.
Pegoraro
L
L
Fierro
M T
MT
Lusso
P
P
Giovinazzo
B
B
Lanino
E
E
Giovarelli
M
M
Matera
L
L
Foa
R
R
D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002648:Child; D002871:Chromosome Banding; D003601:Cytotoxicity Tests, Immunologic; D019309:Epstein-Barr Virus Nuclear Antigens; D005260:Female; D006801:Humans; D007372:Interferons / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D007945:Leukemia, Lymphoid / Q000235:genetics / Q000276:immunology*; D010641:Phenotype; D010996:Pleural Effusion / Q000276:immunology; D013601:T-Lymphocytes; D050378:T-Lymphocytes, Regulatory / Q000502:physiology* / Q000648:ultrastructure; D013997:Time Factors
D000956:Antigens, Viral; D019309:Epstein-Barr Virus Nuclear Antigens; D007376:Interleukin-2; D007372:Interferons