Nonfluorescent negative stain for alanopine dehydrogenase activity on starch gels.
A two-step procedure for negative staining NADH-dependent alanopine dehydrogenase (ALPDH) activity on starch gels is described, using nitroblue tetrasolium and phenasine methosulfate. This method gives a blue background with unreacted NADH, and white areas where ALPDH is located. Using this technique the first reliable evidence of the existence of true ALPDH isoenzymes is obtained.
Manchenko
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D000818:Animals; D004592:Electrophoresis, Starch Gel; D008773:Methylphenazonium Methosulfate; D009580:Nitroblue Tetrazolium; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000302:isolation & purification*; D011077:Polychaeta / Q000201:enzymology; D013194:Staining and Labeling
D009580:Nitroblue Tetrazolium; D008773:Methylphenazonium Methosulfate; D000587:Oxidoreductases Acting on CH-NH Group Donors; C030014:alanopine dehydrogenase