Ribonucleotides are channeled into a mixed DNA-RNA polymer by permeabilized hamster cells.
Permeabilized CHEF/18 hamster cells incorporate label from [3H] cytidine diphosphate (CDP) into material that we had designated as DNA. Spyrou and Reichard reported this label was incorporated only into RNA. To resolve this discrepancy the studies reported here were performed. We demonstrate incorporation into a DNA polymer with major interspersed RNA sequences. Incorporation into deoxycytidine isolated from this product was little diluted by a great excess of unlabeled dCTP, confirming channeling under these conditions of CDP into polymerized deoxyribonucleotides.
G P
GP
Reddy
E M
EM
Klinge
A B
AB
Pardee
D000818:Animals; D002460:Cell Line; D002463:Cell Membrane Permeability; D006224:Cricetinae; D003565:Cytidine Diphosphate / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D012313:RNA / Q000096:biosynthesis*; D012265:Ribonucleotides / Q000378:metabolism*
D012265:Ribonucleotides; D003565:Cytidine Diphosphate; D012313:RNA; D004247:DNA