Novel antigenic specificity involving the blood group antigen, Lea, in combination with onco-developmental antigen, SSEA-1, recognized by two monoclonal antibodies to human milk-fat globule membranes.
Two monoclonal antibodies to human milk-fat globule membranes, which recognize an epithelial antigen designated MAM-3c, were found to bind strongly to epithelial glycoproteins derived from non-secretors. Further investigations, using purified glycoproteins and structurally defined oligosaccharides, established that the optimal antigenic structure for both antibodies involves the Type 1 based blood group antigen, Lea, in combination with the Type 2 based onco-developmental antigen, SSEA-1, (Formula: see text) as in lacto-N-difucohexaose II. The antibodies may also react with the corresponding monofucosyl structures lacking the 3- or 4- linked fucose residues and to a lesser extent with the afucosyl tetrasaccharide sequence as in lacto-N-tetraose. The Lea and SSEA-1 antigens are known to occur on human epithelial glycoproteins. However, this is the first report of an antigenic specificity involving a combination of the Type 1 and Type 2 based fuco-oligosaccharides and occurring on epithelial glycoproteins.
H C
HC
Gooi
N J
NJ
Jones
E F
EF
Hounsell
P
P
Scudder
J
J
Hilkens
J
J
Hilgers
T
T
Feizi
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000276:immunology*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004064:Digestive System / Q000276:immunology; D004848:Epithelium / Q000276:immunology; D000939:Epitopes / Q000276:immunology*; D005260:Female; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000276:immunology; D007983:Lewis Blood Group Antigens / Q000276:immunology*; D008470:Meconium / Q000276:immunology; D008895:Milk, Human / Q000276:immunology*; D009844:Oligosaccharides / Q000276:immunology; D010048:Ovarian Cysts / Q000276:immunology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000939:Epitopes; D006023:Glycoproteins; D007071:Immunoglobulin A, Secretory; D007983:Lewis Blood Group Antigens; D009844:Oligosaccharides