Fast and sensitive detection of protein and DNA bands by treatment with potassium permanganate.
A silver stain technique using treatment with potassium permanganate for visualisation of proteins and DNA separated by gel electrophoresis was developed and applied both to the very thin (thickness 0.1-0.2 mm) and to the usual 1-2 mm thick gels. The technique is reproducible, only stable chemicals are used and it is shortened to about 1 h even for the 1 mm gel (30 min for the 0.2 mm gel). It is applicable to gels thicker than 2 mm with somewhat longer washes. Amounts as low as 2 X 10(-10) g of protein per band have been detected. Protein bands of different colours are obtained. The technique has been used with success in continuous, discontinuous and 2D-gel systems. Bands have been detected which were not observed when the other silver staining techniques were used.
Ansorge
W
W
D004247:DNA / Q000032:analysis*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D011196:Potassium Permanganate; D011506:Proteins / Q000032:analysis*; D012834:Silver; D013194:Staining and Labeling
D011506:Proteins; D011196:Potassium Permanganate; D012834:Silver; D004247:DNA