Effect of fibronectin on IgA-mediated uptake of type III group B streptococci by phagocytes.
Previous studies have shown that a type-specific IgA monoclonal antibody alone or in combination with fibronectin (Fn) enhances protective efficacy in two animal models of group B streptococcal infection. To investigate the mechanisms by which IgA mediates protection, the effects of Fn on phagocytosis of group B streptococci (GBS) opsonized with a type III-specific IgA monoclonal antibody were examined. Specific IgA alone or in combination with Fn did not promote the phagocytosis of GBS by polymorphonuclear leukocytes (PMNL). Fibronectin also had no significant effect on phagocytosis of IgA-opsonized GBS by monocytes. Specific IgA alone promoted phagocytosis of GBS by culture-derived macrophages in a dose-dependent fashion. Fibronectin enhanced macrophage uptake of the GBS opsonized in a suboptimal concentration of specific IgA (phagocytic index = 2.32 +/- 0.56 vs. 3.26 +/- 0.48 with Fn; P less than .05). These data suggest that protection against GBS in neonatal rats by a combination of Fn and specific IgA is mediated by macrophages rather than by PMNL or monocytes. Fibronectin may have a critical role in host defense at sites where IgA and macrophages predominate.
Department of Pathology, University of Utah School of Medicine, Salt Lake City 84132.
K D
KD
Yang
J F
JF
Bohnsack
M M
MM
Hawley
N H
NH
Augustine
W A
WA
Knape
M L
ML
Egan
D G
DG
Pritchard
H R
HR
Hill
D000911:Antibodies, Monoclonal / Q000276:immunology; D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D005353:Fibronectins / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D009000:Monocytes / Q000276:immunology; D009504:Neutrophils / Q000276:immunology; D009895:Opsonin Proteins; D010587:Phagocytosis; D013292:Streptococcus agalactiae / Q000276:immunology*
D000911:Antibodies, Monoclonal; D005353:Fibronectins; D007070:Immunoglobulin A; D009895:Opsonin Proteins; D002374:Catalase