Ultrastructure of spinal efferents to the lateral reticular nucleus: an EM study using anterograde transport of WGA-HRP complex.
Anterograde transport of lectin-conjugated horseradish peroxidase and subsequent incubation with tetramethylbenzidine were employed to label the spinal terminals within the feline lateral reticular nucleus (NRL) for ultrastructural identification. Quantitative studies demonstrated that compared to the unlabelled terminals the spinal boutons were more than twice as large and contained fewer synaptic vesicles. Most of the synaptic contacts of the labelled terminals were located on dendritic shafts but contacts on dendritic spines as well as perikarya were also present. In four cases (all with cervical injections), the postsynaptic cells could be studied in the transversal sections of the medulla in the nuclear plane. The neurons were large and elongated with longest and shortest diameters of about 60 X 30 microns, belonging to the largest category of cells within the NRL. The observations were discussed and related to the findings made in other studies of the NRL.
Westman
J
J
Danckwardt-Lillieström
N
N
Dietrichs
E
E
Svensson
B A
BA
Walberg
F
F
D000818:Animals; D001692:Biological Transport; D001933:Brain Stem / Q000033:anatomy & histology* / Q000648:ultrastructure; D002415:Cats; D006735:Horseradish Peroxidase; D037102:Lectins; D008854:Microscopy, Electron; D009413:Nerve Fibers, Myelinated / Q000648:ultrastructure; D009475:Neurons, Afferent / Q000648:ultrastructure*; D013116:Spinal Cord / Q000033:anatomy & histology* / Q000648:ultrastructure; D013569:Synapses / Q000648:ultrastructure; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate
D037102:Lectins; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D006735:Horseradish Peroxidase