In vitro systems for inducers of cartilage and bone development.
Murine condylar cartilages of perinatal mice were cultured in the form of organ or tissue cultures on top of collagen sponges in medium containing 10% fetal calf serum. Cultures were treated with increasing concentrations of recombinant human growth hormone, recombinant IGF-I, insulin, TGF beta-1, FGF and recombinant fragments of human parathyroid hormone. All the above agents [except for the fragments rPTH (28-48) and (53-84)] were found to be mitogenic as they accelerated the proliferative activity of the condylar stem cells. IGF-I and insulin also stimulated the synthesis of cartilage proteoglycans. Growth hormone also supported de novo osteogenesis in vitro.
Rappaport Family Institute for Research in the Medical Sciences, Faculty of Medicine, Technion-Israel Institute of Technology, Haifa.
M
M
Silbermann
D000818:Animals; D002356:Cartilage / Q000187:drug effects / Q000196:embryology / Q000254:growth & development*; D046508:Culture Techniques; D006133:Growth Substances / Q000494:pharmacology*; D051379:Mice; D010012:Osteogenesis / Q000187:drug effects* / Q000502:physiology; D011509:Proteoglycans / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000494:pharmacology
D006133:Growth Substances; D011509:Proteoglycans; D011994:Recombinant Proteins