Serologic and T cell recognition of truncated transplantation antigens encoded by in vitro deleted class I major histocompatibility genes.
Gene constructions encoding a leader sequence and the C2, transmembrane, and intracytoplasmic exons of both H-2Ld (pLd-C2) and H-2Dd (pDd-C2) were expressed as cell surface proteins when stably introduced into mouse L cells. Distinct proteins of 17.5 and 16 kd precipitated from pDd-C2 transformants reacted with a C2 domain-specific monoclonal antibody, whereas the pLd-C2 transformants expressed a single 19 kd glycoprotein. A xenoantiserum specific for the exon VI/VIII fusion peptide of H-2Dd precipitated only the 16 kd product, suggesting that this protein was derived from an alternately spliced transcript lacking exon VII of the native gene. CTL specifically recognized the pLd-C2 and pDd-C2 gene products, indicating the existence of CTL reactive against polymorphic determinants in the C2 domain of class I antigens.
McCluskey
J
J
Bluestone
J A
JA
Coligan
J E
JE
Maloy
W L
WL
Margulies
D H
DH
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis / Q000235:genetics; D002872:Chromosome Deletion; D004247:DNA / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005802:Genes, MHC Class II; D006183:H-2 Antigens / Q000032:analysis / Q000235:genetics*; D007739:L Cells / Q000378:metabolism; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D012313:RNA / Q000378:metabolism; D011995:Recombination, Genetic; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000378:metabolism; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D000954:Antigens, Surface; D006183:H-2 Antigens; C024755:H-2K(K) antigen; D008565:Membrane Proteins; D012313:RNA; D004247:DNA