A human cell-surface antigen defined by a monoclonal antibody and controlled by a gene on human chromosome 1.
An antigen expressed by most human cells, but not erythrocytes, has been defined by a murine monoclonal antibody, TRA-2-10. This antigen is expressed on the surface of human-mouse somatic cell hybrids, and segregation analysis indicates that it is controlled by a gene located on human chromosome 1. From lysates of most human cells, surface-labelled with 125I, TRA-2-10 immunoprecipitates two polypeptides with molecular weights in the range of about 55 000 to 73 000 depending upon the cell line. Since the TRA-2-10 polypeptides from a fibroblast cell strain and a hepatoma cell line from one individual differ, we conclude that the observed difference in molecular weight has an epigenetic origin.
P W
PW
Andrews
B B
BB
Knowles
M
M
Parkar
B
B
Pym
K
K
Stanley
P N
PN
Goodfellow
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000235:genetics*; D002460:Cell Line; D002469:Cell Separation; D002874:Chromosome Mapping; D002900:Chromosomes, Human, 1-3; D000939:Epitopes / Q000235:genetics; D005434:Flow Cytometry; D006801:Humans; D006825:Hybridomas; D051379:Mice; D011233:Precipitin Tests; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000939:Epitopes