Mucosubstance histochemistry of the normal mucosa and epithelial neoplasms of the large intestine.
Histochemical properties of mucosubstances in normal mucosa and neoplasms of 100 operated cases with colonic carcinoma were investigated by using several new methods, such as paradoxical concanavalin A (con A) staining and modified PAS reactions for sialic acids. Acidity of goblet cell-type mucin (GCM) of the normal mucosa varied with the depth of the crypt, as well as with different segments of the large intestine, whereas surface coat-type mucin (SCM) mainly consisted of sulfomucin throughout the large intestine. In addition, the PAS reactivity revealing the presence of O-acetylated sialic acid and the labile class III con A reactivity were demonstrated as hallmarks characterizing the colonic GCM. In carcinoma tissues, sialomucin was more abundant than in the normal mucosa. Goblet-type tumor cells were found in 59 cases. Moreover, O-acetylated sialic acid and the labile class III con A reactivity persisted in GCM of the goblet-type tumor cells. GCM of the adjacent mucosa of the neoplasms showed a decrease in sulfomucin in 95 cases and a marked increase in the labile class III con A reactivity in 97 cases, while the histochemical properties of SCM in this region remained unchanged.
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D000328:Adult; D000818:Animals; D003106:Colon / Q000032:analysis*; D003110:Colonic Neoplasms / Q000032:analysis*; D003111:Colonic Polyps / Q000032:analysis; D004848:Epithelium / Q000032:analysis; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D009077:Mucins / Q000032:analysis*; D010503:Periodic Acid-Schiff Reaction; D051381:Rats; D012794:Sialic Acids / Q000032:analysis; D013194:Staining and Labeling
D009077:Mucins; D012794:Sialic Acids; C012564:sulfomucin