Receptor-stimulated actin polymerization requires cytoplasmic acidification in human PMNs.
Cytoplasmic alkalinization has received considerable attention as a regulatory event In cell growth, transformation and signal transduction (Busa, W.B. (1986) Annu. Rev. Physiol. 48, 389-402 and Moolenar, W.H. (1986) Annu. Rev. Physiol. 48, 363-376). In contrast the current paper presents evidence for the role of an early cytoplasmic acidification, during signal transduction in the polymorphonuclear leukocyte (PMN). Following PMN stimulation with a chemotactic peptide, there is a significant decrease in cytoplasmic pH concomitant with a dramatic increase in cytoskeletal actin. The data indicate that this drop in pHi is necessary, but not sufficient, for signal transduction leading to cytoskeletal reorganization in these cells.
Tonetti
M
M
Harvard School of Dental Medicine, Forsyth Dental Center, Boston, MA 02115.
Budnick
M
M
Niederman
R
R
D000199:Actins / Q000378:metabolism*; D002465:Cell Movement; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D015398:Signal Transduction
D000199:Actins; D009240:N-Formylmethionine Leucyl-Phenylalanine