CD4 immunoadhesin, but not recombinant soluble CD4, blocks syncytium formation by human immunodeficiency virus type 2-infected lymphoid cells.
Recombinant soluble CD4 (rCD4) has been shown to be an effective inhibitor of human immunodeficiency virus type 1 (HIV-1) and HIV-2 infection of lymphoid cells in vitro. In this report, we characterized the effects of rCD4, the V1V2 fragment of CD4, and the immunoadhesin CD4-immunoglobulin G on syncytium formation between lymphoid cells infected by HIV-1 or HIV-2 and uninfected cells. All three molecules blocked HIV-1-mediated syncytium formation, but only CD4-immunoglobulin G blocked HIV-2-mediated syncytium formation. rCD4 and the V1V2 fragment of CD4 enhanced HIV-2-mediated syncytium formation. These results suggest that the process of cell fusion is significantly different between HIV-1- and HIV-2-infected cells.
Department of Medicine, New England Deaconess Hospital, Harvrd Medical School, Boston, Massachusetts 02215.
I
I
Sekigawa
S M
SM
Chamow
J E
JE
Groopman
R A
RA
Byrn
D015704:CD4 Antigens / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D002470:Cell Survival; D015726:Giant Cells / Q000166:cytology / Q000276:immunology*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology; D015498:HIV-2 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G; D011994:Recombinant Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology
D015704:CD4 Antigens; D007074:Immunoglobulin G; D011994:Recombinant Proteins