Lectin-binding sites in clear cell acanthoma.
Lectin-binding sites in clear cell acanthoma (CCA) were studied using an avidin-biotin complex (ABC) with 9 lectins. Formaldehyde-fixed, paraffin-embedded sections of 7 CCA lesions were employed. Positive stainings, similar to those seen in normal epidermis, were observed on the cell surface in CCA with Ricinus communis agglutinin I (RCA-I), Ricinus communis agglutinin II (RCA-II), and wheat germ agglutinin (WGA). Reduced reactivities were observed with Concanavalin A (ConA) and peanut agglutinin (PNA) in CCA. In some areas of CCA lesions, faint stainings were seen with Ulex europaeus agglutinin I (UEA-I). Capability of staining with soybean agglutinin (SBA) was completely lost in the lesions. With Bandeiraea simplicifolia agglutinin II (BSA-II), cytoplasmic stain was seen in a part of upper and spinous layers in CCA lesions. Dolichos biflorus agglutinin (DBA) did not bind to either CCA or normal epidermis. These results indicate that the lectin-binding sites of proliferating cells of CCA resemble those of epidermal keratinocytes and suggest that CCA is a tumor of epidermal origin.
Department of Dermatology, Keio University School of Medicine, Tokyo, Japan.
M
M
Akiyama
K
K
Hayakawa
Y
Y
Watanabe
T
T
Nishikawa
D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure*; D003208:Concanavalin A / Q000378:metabolism; D005260:Female; D006001:Glycoconjugates / Q000378:metabolism; D006651:Histocytochemistry; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D019887:Peanut Agglutinin; D037121:Plant Lectins; D011975:Receptors, Mitogen / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D012878:Skin Neoplasms / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure*; D030262:Soybean Proteins; D014909:Wheat Germ Agglutinins / Q000378:metabolism; D010835:Phytohemagglutinins
D003208:Concanavalin A; D006001:Glycoconjugates; D037102:Lectins; D019887:Peanut Agglutinin; D037121:Plant Lectins; D011975:Receptors, Mitogen; D012710:Serum Albumin, Bovine; D030262:Soybean Proteins; D014909:Wheat Germ Agglutinins; C017707:Ricinus communis agglutinin-1; C036850:Ulex europaeus lectins; C036554:dolichos biflorus agglutinin; C028824:soybean lectin; D010835:Phytohemagglutinins