Characterization of normal and regenerating bone marrow cells with a panel of monoclonal antibodies.
To obtain baseline data for the diagnosis of residual leukaemia or lymphoma in bone marrow (BM), BM samples of 12 normal adults, 6 patients treated with chemotherapy for solid tumours and 6 patients treated with chemotherapy for leukaemia or lymphoma during remission were assessed with a panel of monoclonal antibodies by fluorescence microscopy immediately following chemotherapy. A large variability between normal individuals was found concerning the percentages of T lymphocytes and T cell subsets, B lymphocytes, HLA-DR+ and OKT10+ cells, due to variation in BM composition and, presumably, peripheral blood contamination. The regenerating BM cells also showed a large variability and, in addition, sometimes very high percentages (up to 19%) of CALLA+, c mu + s mu-, c mu + kappa lambda- and TdT+ cells were found. The interpretation of data on individual BM samples of patients assessed for leukaemia or lymphoma should therefore be related to: the amount of contaminating peripheral blood cells; the cellular composition of the BM samples as determined by May-Grünwald Giemsa staining; baseline data of age-matched normal controls and of regenerating BM of patients without haematological diseases.
H C
HC
Kluin-Nelemans
R L
RL
Bolhuis
B
B
Löwenberg
D
D
Campana
W
W
Sizoo
D000293:Adolescent; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000473:pathology; D001853:Bone Marrow / Q000473:pathology*; D005455:Fluorescent Antibody Technique; D006098:Granulocytes / Q000473:pathology; D006410:Hematopoiesis; D006651:Histocytochemistry; D006801:Humans; D007694:Killer Cells, Natural / Q000473:pathology; D007938:Leukemia / Q000473:pathology*; D008223:Lymphoma / Q000473:pathology*; D008875:Middle Aged; D009369:Neoplasms / Q000473:pathology; D013194:Staining and Labeling; D013601:T-Lymphocytes / Q000473:pathology
D000911:Antibodies, Monoclonal