A new "marker" protein for astrocytes.
A monoclonal antibody (Mab J1-31) has been produced by using human brain homogenate as immunogen in mouse. Double-label immunofluorescence microscopy on cryostat sections of human, rabbit and rat brain, reveals staining of cells that are also stained with antiserum to glial fibrillary acidic protein (GFAP, a commonly used marker protein for astrocytes). However, there is no decrease in staining due to Mab J1-31 in sections incubated in antiserum to GFAP prior to incubation with the J1-31 ascites fluid. Immunoprecipitation of aqueous and detergent extracts of brain homogenate gives a single band at 30K by SDS-PAGE followed by autoradiography. Immunoelectron microscopy shows that the J1-31 antigen is associated with the cytoskeleton. Thus, the Mab J1-31 recognizes a new protein present in GFAP positive cells (astrocytes) in the brain.
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Singh
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Singh
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Malhotra
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000951:Antigens, Neoplasm / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis*; D001253:Astrocytes / Q000276:immunology*; D001345:Autoradiography / Q000379:methods; D001921:Brain / Q000276:immunology; D002490:Central Nervous System / Q000276:immunology; D002531:Cerebellum / Q000276:immunology; D003599:Cytoskeleton / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000276:immunology; D006801:Humans; D006825:Hybridomas; D007381:Intermediate Filament Proteins / Q000276:immunology; D051379:Mice / Q000276:immunology; D008856:Microscopy, Fluorescence / Q000379:methods; D009103:Multiple Sclerosis / Q000276:immunology; D011817:Rabbits; D051381:Rats
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D000939:Epitopes; D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins