The increased release of prostaglandin E2 by Kupffer cells from burned guinea pigs.
Prostaglandin E2 (PGE2) is a very important immunosuppressive substance that is synthesized and released by all macrophages including Kupffer cells (KCs). In this study the changes of PGE2 released by KCs were evaluated in a burned guinea pig model. Prostaglandin E2 was released by KCs from burned guinea pigs at a consistently and significantly high level when stimulated with endotoxin. On postburn day 8, KCs that were cocultured with hepatocytes released significantly less PGE2. There was also a significant decrease in antibody-dependent cell-mediated cytotoxicity on postburn day 1. Kupffer cells can mediate immune suppression in burn injury by a prolonged increased production of PGE2 that has immunosuppressive effects on other cells and by a decreased cytotoxic effect soon after burn injury.
Ogle
C K
CK
Shriners Burns Institute, Cincinnati, OH 45219.
Wu
J Z
JZ
Wood
S
S
Ogle
J D
JD
Alexander
J W
JW
Warden
G D
GD
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity / Q000276:immunology*; D002056:Burns / Q000276:immunology / Q000378:metabolism*; D015232:Dinoprostone / Q000378:metabolism*; D004731:Endotoxins / Q000494:pharmacology; D006168:Guinea Pigs; D007108:Immune Tolerance / Q000276:immunology*; D007728:Kupffer Cells / Q000378:metabolism*; D008297:Male; D013997:Time Factors
D004731:Endotoxins; D015232:Dinoprostone