Evidence for two types of GABA-containing interneurons in the A-laminae of the cat lateral geniculate nucleus: a double-label HRP and GABA-immunocytochemical study.
Neurons containing GABA immunoreactivity were analyzed in the A-laminae of normal cat LGN and of LGN retrogradely labeled with HRP from the visual cortex. In contrast to retrograde labeling of relay cells, GABA+ cells were devoid of HRP label, providing additional evidence for the interneuronal nature of GABAergic cells in the cat LGN. Cell body area measurements showed that the population of GABA+ cells is composed of a large proportion of small (beta) cells and a smaller proportion of medium size (alpha) cells. The proportion of alpha GABA+ cells increases from medial to lateral parts of the A-laminae, resembling a similar medio-lateral increase of physiologically defined Y cells and of morphologically defined type 1 cells in these laminae. This suggests that the alpha and beta GABAergic cells are related to the Y and X geniculo-cortical relay cells, respectively.
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VM
Montero
J
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Zempel
D000818:Animals; D001369:Axons / Q000378:metabolism; D002415:Cats; D002452:Cell Count; D005829:Geniculate Bodies / Q000032:analysis* / Q000166:cytology; D006735:Horseradish Peroxidase; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D007395:Interneurons / Q000032:analysis / Q000145:classification*; D013194:Staining and Labeling; D014793:Visual Cortex / Q000032:analysis / Q000166:cytology; D014795:Visual Pathways / Q000032:analysis / Q000166:cytology; D005680:gamma-Aminobutyric Acid / Q000032:analysis* / Q000276:immunology
D007106:Immune Sera; D005680:gamma-Aminobutyric Acid; D006735:Horseradish Peroxidase