Glucose-evoked recovery of hepatic thyroxine 5'-deiodinase independent of de novo protein synthesis in fasted rat.
The glucose-evoked recovery of Type I thyroxine 5'-deiodinase activity in the hepatic microsomes of fasted rat was not inhibited by either cycloheximide, puromycin or actinomycin D during 3 h after glucose feeding; however, [3H]-leucine uptake by the liver or the hepatic microsomal fraction was significantly inhibited by cycloheximide and puromycin but not by actinomycin D. These results indicate that the glucose-evoked recovery of deiodinase activity may be independent of de novo protein synthesis.
Birmingham V.A. Medical Center, Alabama.
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Nishida
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Pittman
D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D005215:Fasting; D005502:Food; D005947:Glucose / Q000494:pharmacology*; D007328:Insulin / Q000097:blood; D007453:Iodide Peroxidase / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D014176:Protein Biosynthesis; D011691:Puromycin / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013974:Thyroxine / Q000097:blood
D007328:Insulin; D003609:Dactinomycin; D011691:Puromycin; D003513:Cycloheximide; D007453:Iodide Peroxidase; D005947:Glucose; D013974:Thyroxine