A further characterization of acridine-photosensitized inhibition of mast cell degranulation.
The purpose of this study was to further characterize acridine-photosensitized inhibition of mast cell degranulation. Acridine plus UVA radiation (320-400 nm) inhibited degranulation in response to antigen in IgE-sensitized rat serosal mast cells and in response to concanavalin A, which acts by a mechanism similar to antigen-IgE challenge. Removing oxygen from the incubation medium prevented the acridine-photosensitized inhibition of mast cell degranulation in response to 48/80. Acridine plus UVA radiation did not decrease mast cell ATP content, thus excluding inhibition of ATP production as a mechanism for photosensitized inhibition of mast cell degranulation. Although the viability of mast cells, as determined by uptake of trypan blue, was not affected 3 h after treatment with acridine plus UVA radiation, viability decreased by 6 h, and by 22 h 44% of the cells were nonviable. These results indicate that degranulation of mast cells by a variety of agents is inhibited by UVA plus acridine treatment, and that photosensitization requires oxygen and occurs before cytotoxicity.
Gendimenico
G J
GJ
Wellman Laboratories, Department of Dermatology, Harvard Medical School, Massachusetts General Hospital, Boston.
Kochevar
I E
IE
D000166:Acridines / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects / Q000528:radiation effects*; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D007073:Immunoglobulin E / Q000276:immunology; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D014466:Ultraviolet Rays
D000166:Acridines; D003208:Concanavalin A; D007073:Immunoglobulin E; D000255:Adenosine Triphosphate