Expression of the cell proliferation-associated nuclear antigen reactive with the Ki-67 monoclonal antibody by cells of the skeletal system in humans and other species.
The cell proliferation-associated nuclear antigen recognized by the Ki-67 monoclonal antibody was detected immunohistochemically in cells from skeletal tissues of humans, sheep and rabbits. In each case the distribution of Ki-67-positive cells was identical and consistent with the known distribution of dividing cells in growing long bones. The antigen recognized by Ki-67 is detectable only after fixation in cold acetone. In the 5-day-old rabbit, labelling with tritiated thymidine [( 3H]TdR) in vivo, followed subsequently by a combination of Ki-67 immunohistochemistry and autoradiography for [3H]TdR, demonstrated that, in addition to the expression of Ki-67 by the majority of [3H]TdR labelled cells, other cells associated with proliferating populations also expressed the Ki-67 antigen. This suggests that the Ki-67 antibody identifies cycling cells in the skeleton in S-phase, as well as in other phases of the cell cycle. In Ki-67 reactive species, this method is a potentially useful tool for the study of cell proliferation in skeletal tissues in vivo and in vitro, having the advantage of not requiring prior labelling with DNA precursors.
Apte
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Nuffield Department of Orthopaedic Surgery, University of Oxford, Headington, England.
D000096:Acetone; D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis*; D001345:Autoradiography; D001846:Bone Development; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D001842:Bone and Bones / Q000166:cytology* / Q000196:embryology / Q000276:immunology; D002356:Cartilage / Q000276:immunology; D002455:Cell Division; D003080:Cold Temperature; D004247:DNA / Q000096:biosynthesis; D005404:Fixatives; D005865:Gestational Age; D006132:Growth Plate / Q000166:cytology / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen; D011817:Rabbits; D012756:Sheep
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D005404:Fixatives; D019394:Ki-67 Antigen; D000096:Acetone; D004247:DNA