[The effect of endothelial interaction on the binding capacity of DNA acridine orange in hypertension].
Interaction of monocytes and endothelial cells were examined by acridine orange reaction in arteries of normotensive and hypertonic rats. The method is suitable for electron microscope study of DNA template activity. Activity of gen was compared in surface bound and subendothelial and in nonadhesive endothelial cells bound to monocyte. Acridine orange positivity, indicative of genic activity, was not found in few monocytes adhered in arteries of normotensive animals, while 62% of adhesive monocytes of hypertonic and 86% of ones accessed to endothelial space contained the products of acridine-orange-chromatin reaction in its nuclear, showing with it the gene derepression quickly ensuing in acute hypertensive vascular lesions. Only 2% of endothelial cells of pseudo-operated normotensive animals showed acridine orange positivity, while 17% of endothelial cells of hypertonic animals were positive. In the latter animals, 57% of endothelial cells showed acridine orange positivity, if monocytes adhered to their surface. In 84% of monocyte-endothelial pairs connected to each other, the nuclei have activity of same sign. Results show intensive activation of genes of monocytes in relation with adhesion and migration to vascular wall. Presumably, both hypertonia and monocyte adhesion have contributed to the increase of template activity of endothelial cells.
Semmelweis OTE II. Kórbonctani Intézete, Bochum.
T
T
Kerényi
R
R
Lehmann
B
B
Voss
H
H
Jellinek
D000165:Acridine Orange; D000818:Animals; D001665:Binding Sites; D004727:Endothelium / Q000502:physiology / Q000648:ultrastructure; D006973:Hypertension / Q000503:physiopathology*; D008638:Mesenteric Arteries / Q000648:ultrastructure; D009000:Monocytes / Q000502:physiology / Q000648:ultrastructure*; D051381:Rats; D011919:Rats, Inbred Strains
D000165:Acridine Orange
A monocyta endothel interakció hatása a DNS akridin-oranzs kötöképességére hypertoniában.