Characterization of two azurphil granule proteases with active-site homology to neutrophil elastase.
Much of the tissue damage associated with emphysema and other inflammatory diseases has been attributed to the proteolytic activity of neutrophil elastase, a major component of the azurophil granule. Recently, two additional azurophil granule proteins with NH2-terminal sequence homology to elastase were isolated (Gabay, J. E., Scott, R. W., Campanelli, D., Griffith, J., Wilde, C., Marra, M. N., Seeger, M., and Nathan, C. F. (1989) Proc. Natl. Acad. Sci. U.S.A. 86, 5610-5614) and designated azurophil granule protein 7 (AGP7) and azurocidin. Azurocidin and AGP7 represent significant protein components of the azurophil granule, together comprising approximately 15% of the acid-extractable protein as judged by reverse-phase high performance liquid chromatography analysis. AGP7 migrates on sodium dodecyl sulfate-polyacrylamide gel electrophoresis as four distinct glycoforms of molecular mass 28-34 kDa, whereas azurocidin exhibits three predominant bands with molecular mass of 28-30 kDa. Treatment of intact azurophil granules with [3H]diisopropyl fluorophosphate resulted in labeling of elastase, cathepsin G, and AGP7, whereas azurocidin was not labeled. Tryptic mapping of 3H-labeled AGP7 allowed us to identify and sequence the active-site polypeptide that has 70% identity to elastase over 20 residues. The active site peptide of azurocidin was also identified by sequence analysis of tryptic fragments and showed 65% identity to the active site of elastase. Surprisingly, the catalytic serine of azurocidin is replaced by glycine, explaining its inability to label with [3H]diisopropyl fluorophosphate. Thus, we have identified two azurophil proteins closely related to neutrophil elastase, one of which has apparently lost its proteolytic activity due to mutation of the catalytic serine.
Wilde
C G
CG
Invitron Corporation, Redwood City, California 94063.
Snable
J L
JL
Griffith
J E
JE
Scott
R W
RW
D000595:Amino Acid Sequence; D001665:Binding Sites; D002458:Cell Fractionation; D002851:Chromatography, High Pressure Liquid; D003594:Cytoplasmic Granules / Q000201:enzymology*; D006098:Granulocytes / Q000201:enzymology*; D006801:Humans; D007531:Isoflurophate / Q000378:metabolism; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000097:blood* / Q000235:genetics; D010446:Peptide Fragments / Q000302:isolation & purification; D010447:Peptide Hydrolases / Q000097:blood* / Q000235:genetics; D010449:Peptide Mapping; D011485:Protein Binding; D012689:Sequence Homology, Nucleic Acid; D013347:Subcellular Fractions / Q000201:enzymology; D013379:Substrate Specificity; D014357:Trypsin
D010446:Peptide Fragments; D007531:Isoflurophate; D010447:Peptide Hydrolases; D010196:Pancreatic Elastase; D014357:Trypsin