The expression of the sIgD isotype in wild-derived mice.
IgD and IgM are concomitantly expressed on the surface of most mouse B lymphocytes and both molecules serve as receptor for antigen. In this communication we report that in contrast to IgM, which is expressed in a constant manner on the surface of spleen B lymphocytes of different laboratory and wild-derived mice, IgD expression is variable among the spleen cells of wild-derived mice. SPE, SEI, and SFM mice belonging to the Mus 3 subgroup show a fluorescence profile characterized by a marked diminution in the population of B lymphocytes expressing the IgD isotype; in addition, these cells have a low sIgD density on their membranes. These findings were confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of the iodinated membrane proteins. Polyclonal in vitro activation with lipopolysaccharide increases the frequency of surface IgD (sIgD)-bearing spleen cells and sIgD density in the SPE strain but decreases both the frequency and the density of IgD bearing cells in the BALB/c strain. This result suggests that delta gene expression is regulated differently in SPE and BALB/c mice. In addition, genetic analysis of sIgD expression in (BALB/c X SPE)F1 hybrids suggests that the proportion of sIgD-bearing cells and sIgD density are independently regulated.
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D000828:Animal Population Groups / Q000276:immunology*; D000818:Animals; D000835:Animals, Wild / Q000276:immunology*; D000939:Epitopes / Q000032:analysis; D005362:Ficoll / Q000031:analogs & derivatives / Q000276:immunology; D007126:Immunoglobulin Allotypes / Q000032:analysis* / Q000235:genetics; D007072:Immunoglobulin D / Q000032:analysis* / Q000235:genetics; D007958:Leukocyte Count; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D011947:Receptors, Antigen, B-Cell / Q000032:analysis* / Q000235:genetics; D013045:Species Specificity; D013154:Spleen; D014301:Trinitrobenzenes / Q000276:immunology
D000939:Epitopes; D007126:Immunoglobulin Allotypes; D007072:Immunoglobulin D; D008070:Lipopolysaccharides; D011947:Receptors, Antigen, B-Cell; C020529:TNP-ficoll; D014301:Trinitrobenzenes; D005362:Ficoll