The nature of DT-diaphorase (EC 1.6.99.2) activity in plasma membrane of astrocytes in primary cultures.
This is the confirmation of an earlier indication (Mersel, M., Malviya, A.N., Hindelang, C. and Mandel, P. (1984) Biochim. Biophys. Acta 778, 144-154) that the plasma membrane of astrocytes in primary cultures is endowed with DT-diaphorase (EC 1.6.99.2) activity. It is observed that the NADPH-2,6-dichloroindophenol diaphorase activity found in the isolated plasma membrane is not inhibited by dicoumarol. DT-diaphorase-type activity is also observed on the cell surface employing dichloroindophenol as external electron acceptor and it is found to be a dicoumarol-sensitive NADH dehydrogenase.
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Malviya
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Mandel
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Mersel
D000818:Animals; D000968:Antimycin A / Q000031:analogs & derivatives / Q000494:pharmacology; D001253:Astrocytes / Q000201:enzymology*; D002462:Cell Membrane / Q000201:enzymology*; D002478:Cells, Cultured; D001728:Dicumarol / Q000494:pharmacology; D016660:NAD(P)H Dehydrogenase (Quinone); D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D011808:Quinone Reductases / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D012402:Rotenone / Q000494:pharmacology
D012402:Rotenone; C032456:antimycin; D000968:Antimycin A; D001728:Dicumarol; D009247:NADH, NADPH Oxidoreductases; D016660:NAD(P)H Dehydrogenase (Quinone); D011808:Quinone Reductases