Cloning and sequencing of cDNA of Sarcophaga peregrina humoral lectin induced on injury of the body wall.
A previous paper described the purification of a lectin induced in the hemolymph of larvae of Sarcophaga peregrina (flesh-fly) on injury of their body wall (Komano, H., Mizuno, D., and Natori, S. (1980) J. Biol. Chem. 255, 2919-2924). This paper describes cDNA cloning and the complete nucleotide sequence of the gene for Sarcophaga lectin. Although active lectin consists of alpha and beta subunits in a molar ratio of 2:1, the fat body of injured larvae was found to contain only mRNA for the alpha subunit, suggesting that these two subunits are derived from a common gene and that the alpha subunit is converted to the beta subunit post-translationally. The alpha subunit was found to consist of 260 amino acid residues with an additional signal sequence of 19 or 23 amino acid residues.
H
H
Takahashi
H
H
Komano
N
N
Kawaguchi
N
N
Kitamura
S
S
Nakanishi
S
S
Natori
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D004175:Diptera / Q000276:immunology*; D006458:Hemolymph / Q000276:immunology; D019476:Insect Proteins; D007814:Larva / Q000276:immunology; D037102:Lectins / Q000235:genetics* / Q000302:isolation & purification; D037181:Lectins, C-Type; D046911:Macromolecular Substances; D008970:Molecular Weight; D011061:Poly A / Q000235:genetics; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D014158:Transcription, Genetic
C043030:20kDa lectin protein, Sarcophaga; D019476:Insect Proteins; D037102:Lectins; D037181:Lectins, C-Type; D046911:Macromolecular Substances; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA; D004262:DNA Restriction Enzymes