Inhibitory effect of tannins on reverse transcriptase from RNA tumor virus.
Twenty-four tannins and related compounds were examined for inhibition against reverse transcriptase from RNA tumor virus. Hydrolyzable tannins showed a potent inhibitory effect comparable to nitidine, in the presence of polyadenylic acid-oligothymidylic acid as a template-primer. A lesser inhibitory activity was observed for the monomeric ellagitannins, gallotannins, and nitidine using polycytidylic acid-oligodeoxyguanylic acid as a template-primer, whereas the inhibitory activity of dimeric ellagitannins was almost as potent as that observed for these compounds in the polyadenylic acid-oligothymidylic acid directed reaction. Inhibition by tannins was reversed by the addition of either template-primer or enzyme, suggesting that the inhibition is due to the interaction of tannins with both of them.
N
N
Kakiuchi
M
M
Hattori
T
T
Namba
M
M
Nishizawa
T
T
Yamagishi
T
T
Okuda
D000818:Animals; D001354:Avian Leukosis Virus / Q000201:enzymology*; D009189:Avian Myeloblastosis Virus / Q000201:enzymology*; D002417:Cattle; D004257:DNA Polymerase II / Q000378:metabolism; D007700:Kinetics; D019684:Magnoliopsida / Q000032:analysis; D018894:Reverse Transcriptase Inhibitors; D013634:Tannins / Q000494:pharmacology*; D013950:Thymus Gland / Q000201:enzymology
D018894:Reverse Transcriptase Inhibitors; D013634:Tannins; D004257:DNA Polymerase II