Immunohistochemical co-localization of S-100b and the glial fibrillary acidic protein in rat brain.
A rabbit antiserum against purified bovine brain S-100b protein was produced and characterized by immunoassay and immunoblot analysis of electrophoretically resolved soluble brain proteins. Fluorescent immunohistochemistry was conducted in order to determine the cellular localization of the S-100b immunoreactivity. Double immunohistofluorescent experiments on adult rat brain tissue sections with the rabbit antiserum to S-100b and a rat monoclonal antibody to the glial fibrillary acidic protein resulted in immunolabelling of the same cells. This finding determines a strict astroglial localization of the S-100b immunoreactivity. In addition, the immunolabelling of astrocyte perikarya and processes by the S-100b immunohistochemistry is consistent with a cytoplasmic location of S-100b. In contrast, the glial fibrillary acidic protein immunohistochemistry predominantly labeled the fine fibrillary processes of the cells. The present report suggests that S-100b immunohistochemistry is of use for the specific identification and morphological description of astrocytes in the rat brain.
B E
BE
Boyes
S U
SU
Kim
V
V
Lee
S C
SC
Sung
D000818:Animals; D000911:Antibodies, Monoclonal; D001253:Astrocytes / Q000032:analysis; D001923:Brain Chemistry; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis*; D007106:Immune Sera / Q000032:analysis; D008297:Male; D009474:Neurons / Q000032:analysis; D011817:Rabbits; D051381:Rats; D009418:S100 Proteins / Q000032:analysis*; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D005904:Glial Fibrillary Acidic Protein; D007106:Immune Sera; D009418:S100 Proteins