Binding in vitro of vasoactive intestinal peptide on isolated acini of rat parotid glands.
Binding of 125I-labelled vasoactive intestinal peptide (VIP) to rat parotid acini was saturable, temperature-dependent and reversible, and reflected interaction with a single class of binding sites. Parotid glands possessed approx. 400 fmol binding sites per mg protein and binding of the tracer to these sites could be inhibited by VIP [concentration for half-maximal effect (KD), 24 nM], by the peptide histidine isoleucine (KD, 140 nM), by secretin (KD, 470 nM) and by the human pancreatic growth hormone-releasing factor (hpGRF; KD, 3200 nM). In the same acini preparation, 10 microM VIP also stimulated amylase release 4-fold and increased cyclic AMP 11-fold. Thus, VIP might be a neurotransmitter in the rat parotid gland.
J P
JP
Dehaye
J
J
Christophe
F
F
Ernst
P
P
Poloczek
P
P
Van Bogaert
D000681:Amylases / Q000378:metabolism; D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D000242:Cyclic AMP / Q000378:metabolism; D013007:Growth Hormone-Releasing Hormone / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D010306:Parotid Gland / Q000378:metabolism*; D010446:Peptide Fragments / Q000494:pharmacology; D010451:Peptide PHI; D010455:Peptides / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012633:Secretin / Q000494:pharmacology; D013997:Time Factors; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism* / Q000494:pharmacology
D010446:Peptide Fragments; D010451:Peptide PHI; D010455:Peptides; D012633:Secretin; D014660:Vasoactive Intestinal Peptide; D013007:Growth Hormone-Releasing Hormone; D000242:Cyclic AMP; D000681:Amylases