Isolation and characterization of four forms of trehalase from rabbit kidney cortex.
Four forms of renal trehalase were isolated and purified to homogeneity. Hydrophobic interaction chromatography separated two forms; A-form and B-form. Both forms were subdivided further on Con A-Sepharose and were stained with periodic acid-Schiff reagent, indicating that they are glycoproteins. The four forms of renal trehalase showed no significant difference in Km values for trehalose and K1 values for various inhibitors. The optimum pH of the four forms was pH 6.0 in phosphate buffer. Apparent molecular weights on gel filtration of the four forms were the same, 175,000. Furthermore, the four forms showed the same antigenicity on double immunodiffusion. However, isoelectric point (pI), susceptibility to HgCl2, stability at -80 degrees C and Na+ activation behavior were different. Glycoprotein forms were more susceptible to HgCl2 and showed lower Na+ activation than nonglycoprotein forms. The pI of less hydrophobic forms (A1, A2) was more acidic than that of more hydrophobic forms (B1, B2). On the basis of these results, it is likely that four forms of renal trehalase are "isozymes."
Nakano
M
M
Sacktor
B
B
D000818:Animals; D002384:Catalysis; D003080:Cold Temperature; D004789:Enzyme Activation / Q000187:drug effects; D000939:Epitopes; D005260:Female; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D007672:Kidney Cortex / Q000201:enzymology*; D008297:Male; D008627:Mercuric Chloride / Q000494:pharmacology; D008970:Molecular Weight; D011817:Rabbits; D012964:Sodium / Q000494:pharmacology; D014198:Trehalase / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism
D000939:Epitopes; D008627:Mercuric Chloride; D012964:Sodium; D014198:Trehalase