Immunoblotting and ELISA analysis of HSV-1 and HSV-2-specified polypeptides by using an immunoblocking method.
Herpes Simplex Virus (HSV) specified polypeptide antigens were analyzed by immunoblotting and enzyme-linked immunosorbent assay (ELISA) methods. In general, the virus specified proteins showed marked cross-reactivity. However, the HSV-1 specified glycoprotein C could be detected by HSV-1 infected rabbit antiserum only. Two low molecular weight polypeptides showed considerable reactivity with HSV-2 induced immune sera as revealed by an immunoblocking modification of the immunoblotting method. These proteins may prove useful in the serological diagnosis of HSV Type 1 and Type 2 infections.
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Lehtinen
D000936:Antigen-Antibody Complex / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D005455:Fluorescent Antibody Technique; D007120:Immunochemistry; D008970:Molecular Weight; D018139:Simplexvirus / Q000032:analysis* / Q000276:immunology; D014764:Viral Proteins / Q000032:analysis*
D000936:Antigen-Antibody Complex; D000956:Antigens, Viral; D000939:Epitopes; D014764:Viral Proteins