The receptor-destroying enzyme of influenza C virus is neuraminate-O-acetylesterase.
The nature of the receptor-destroying enzyme (RDE) of influenza C virus has been elucidated by analyzing its effect on the haemagglutination inhibitors rat alpha 1-macroglobulin (RMG) and bovine submandibulary mucin (BSM), respectively. The inhibitory activity of both compounds is abolished by incubation with influenza C virus. After inactivation, RMG and BSM were found to contain reduced amounts of N-acetyl-9-O-acetylneuraminic acid (Neu5,9Ac2) and increased amounts of N-acetylneuraminic acid (Neu5Ac). H.p.l.c. analysis revealed that purified Neu5,9Ac2 is converted to Neu5Ac by incubation with influenza C virus. These results demonstrate that RDE of influenza C virus is neuraminate-O-acetylesterase [N-acyl-9(4)-O-acetylneuraminate O-acetylhydrolase (EC 3.1.1.53)]. The data also indicate that haemagglutination-inhibition (HI) by RMG and BSM and most likely virus attachment to cell surfaces involves binding of influenza C virus to Neu5,9Ac2.
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G
Herrler
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R
Rott
H D
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Klenk
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Müller
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Shukla
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Schauer
D000115:Acetylesterase / Q000502:physiology*; D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000032:analysis*; D002417:Cattle; D009077:Mucins / Q000378:metabolism; D009438:Neuraminic Acids / Q000378:metabolism*; D009439:Neuraminidase / Q000502:physiology; D009975:Orthomyxoviridae / Q000201:enzymology*; D011991:Receptors, Virus / Q000378:metabolism*; D000511:alpha-Macroglobulins / Q000378:metabolism
D009077:Mucins; D009438:Neuraminic Acids; D011991:Receptors, Virus; D000511:alpha-Macroglobulins; D002265:Carboxylic Ester Hydrolases; D000115:Acetylesterase; C046298:sialate O-acetylesterase; D009439:Neuraminidase