[Production and characterization of monoclonal antibody to uterine cervical cancer cell line SKG-IIIa].
By means of an immunization procedure using intact living cells, the established uterine cervical epidermoid cancer cell line SKG-IIIa, a monoclonal antibody (Mab) which reacts with uterine cervical epidermoid cancer cells was obtained. The Mab was of IgG2a subclass. Immunohistochemically the Mab reacted with uterine cervical epidermoid cancer cells (17 out of 25). At the same time, it reacted with a basal layer of normal squamous epithelium, surface mucus secreting cells and parietal cells in stomach and kidney uriniferous tubules. The Mab reacted with the cell surface epitope on HeLa cells to the same extent as on SKG-IIIa cells, but on CCD18-Lu cells (normal diploid) to about one-half as much. The results of an inhibition test using a competitive reaction between Mab and conventional polyclonal antibody indicated that the Mab didn't react with 39 kinds of human serum proteins such as albumin, transferrin, IgG, fibrinogen, lysozyme and some tumor markers. Immunohistochemical staining also revealed that the Mab didn't recognize human blood group substances (A,B,H type, Lewis's a,b type). Treatment of SKG-IIIa cells with a mixture of glycosidases neuraminidase or periodate didn't change the reactivity to the Mab, suggesting that the epitope didn't reside in sugar chains.
S
S
Nozawa
S
S
Narisawa
Y
Y
Takayama
S
S
Kurihara
M
M
Mukai
D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000915:Antibody Affinity; D001667:Binding, Competitive; D001789:Blood Group Antigens / Q000276:immunology; D001798:Blood Proteins / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000276:immunology*; D002459:Cell Fusion; D002460:Cell Line; D000939:Epitopes; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007124:Immunoenzyme Techniques; D002583:Uterine Cervical Neoplasms / Q000276:immunology*
D000911:Antibodies, Monoclonal; D001789:Blood Group Antigens; D001798:Blood Proteins; D000939:Epitopes