Sequences in the gag-pol-5'env region of avian leukosis viruses confer the ability to induce osteopetrosis.
DNA sequences encoding the genomes of three subgroup E avian leukosis viruses have been molecularly cloned. Virus recovered from one of these cloned DNAs (pRAV-0) caused no osteopetrosis while virus recovered from the second (lambda NY203) caused late onset osteopetrosis and virus recovered from the third (lambda NTRE-2) caused intermediate onset osteopetrosis. Restriction endonuclease fragments of the cloned viral DNAs were used to construct recombinant viruses that could be used to test for the role of gag-pol-5'env and 3'env-LTR sequences in the induction of osteopetrosis. The results of the pathogenicity tests indicate that gag-pol-5'env sequences confer the ability to induce osteopetrosis while 3'env-LTR sequences influence the time of onset and the severity of osteopetrosis.
Shank
P R
PR
Schatz
P J
PJ
Jensen
L M
LM
Tsichlis
P N
PN
Coffin
J M
JM
Robinson
H L
HL
D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D001483:Base Sequence; D002460:Cell Line; D002645:Chickens; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005260:Female; D005814:Genes, Viral; D008297:Male; D010022:Osteopetrosis / Q000382:microbiology*; D012194:RNA-Directed DNA Polymerase / Q000235:genetics; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics; D015678:Viral Structural Proteins
D014764:Viral Proteins; D015678:Viral Structural Proteins; D012194:RNA-Directed DNA Polymerase; D004262:DNA Restriction Enzymes