DNA content in nerve-cell nucleus. A biochemical and cytophotometric study of the rat cerebrum.
The cerebral nuclei of 30-day-old rats were separated by a two-step gradient centrifugation into the fractions of large and small nuclei. The DNA content per nucleus was determined biochemically and cytophotometrically in these fractions as well as in the non-separated cerebellar and liver-cell nuclei used as reference cells. The DNA content of large and/or mainly neuronal, and the small and/or glial cell-enriched nuclei were 6.74 +/- 0.51 and 6.21 +/- 0.30 pg, respectively, and in the cerebellum 6.10 +/- 0.28 pg per nucleus. The DNA-content values determined cytophotometrically in Feulgen-stained samples of large and small bulk isolated cortical nuclei ranged within the diploid limits, indicated by a part of a liver-cell population measured in parallel. No evidence for the large scale existence of an "extra-DNA" reported earlier [Bregnard, Knuesel and Kuenzle (1975) Histochemie 43, 59-61; Bregnard, Kuenzle and Ruch (1977) Expl Cell Res. 107, 151-157; Kuenzle, Bregnard, Hübschner and Ruch (1978) Expl Cell Res. 113, 151-160] in cortical neurons has thus been obtained.
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Mares
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Crkovská
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Marsak
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Stípek
D000818:Animals; D001923:Brain Chemistry; D002467:Cell Nucleus / Q000032:analysis*; D002531:Cerebellum / Q000032:analysis; D004396:Coloring Agents; D003592:Cytophotometry; D004247:DNA / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D012394:Rosaniline Dyes; D013194:Staining and Labeling
D004396:Coloring Agents; C007722:Feulgen stain; D012394:Rosaniline Dyes; D004247:DNA