[Immunomorphological study of the distribution of prekeratin with a molecular weight of 49 kilodaltons in various epithelial cells in rats].
Cryostat sections of various tissues of rat were stained using an indirect immunofluorescent method with monoclonal antibody against individual prekeratin with the molecular mass of 49 kilodalton (PK-49). Connective tissue endothelial cells, neurons, glia, haematopoetic tissue and smooth muscles were completely negative in this test. 46 morphological variants of epithelial structures were investigated. PK-49 was absent from all the stratified epithelia (epidermis, hair folliculi, oesophagus) but was expressed in virtually all simple epithelia of endodermal origin (exceptions: squamous lung alveolar epithelium and germinative epithelium of testis). There were negative (kidney tubules) as well as positive (bladder, mammary, glands) cell elements among mesodermal and ectodermal simple epithelia. High specificity of individual PK in respect to morphological variants of epithelia points out to the important role played by prekeratin-type intermediate filaments in morphogenesis.
Gel'shteĭn
V I
VI
Chipysheva
T A
TA
Troianovskiĭ
S M
SM
Bannikov
G A
GA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D004848:Epithelium / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D007381:Intermediate Filament Proteins / Q000032:analysis / Q000378:metabolism*; D007633:Keratins / Q000032:analysis / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D011498:Protein Precursors / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D007381:Intermediate Filament Proteins; D011498:Protein Precursors; C004818:prekeratin; D007633:Keratins
Immunomorfologicheskoe izuchenie raspredeleniia prekeratina s molekuliarnoĭ massoĭ 49 kilodal'ton v kletkakh razlichnykh épiteliev krys.