[Capillary isotachophoresis--a new method in drug analysis. 2. Analytical capillary isotachophoresis of the synthetic peptide substance P].
For the synthetic undecapeptide substance P being object of actual pharmacological research and a potential peptide-drug experiments were carried out for the assessment of cation analyses by means of capillary isotachophoresis for the purpose of the development of test methods for pharmaceutical judgments of quality. Best conditions exist at a pH range from 6.2 to 6.3 of the leading electrolyte of the discontinuous electrolyte system by using of the counter ion morpholinoethansulfonic acid. The results confirm the high separation efficiency and rate of information of such analyses in the field of peptides. They served the control of purification methods for substance P as well as the determination of the contents in preparations for this peptide including simultaneously in case of need the preservative benzalkonium chloride with sufficient reproducibility (rel. S.D. 0.7-2.7% for substance P and less than or equal to 3% for benzalkonium chloride). It is also possible to estimate impurities and products of decomposition respectively down to the level of 0.1-1% and following that to make statements about the stability.
Jannasch
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D001548:Benzalkonium Compounds; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D004586:Electrophoresis; D006863:Hydrogen-Ion Concentration; D013056:Spectrophotometry, Ultraviolet; D013373:Substance P / Q000032:analysis*
D001548:Benzalkonium Compounds; D013373:Substance P
Die Kapillarisotachophorese--eine neue Methode in der Arzneimittelanalytik. 2. Mitteilung: Analytische Kapillarisotachophorese des synthetischen Peptid-Wirkstoffes Substanz P.