Recognition of peptide orientation: studies with angiotensin II in the guinea-pig.
Antipeptide sera of defined subspecificities were obtained when angiotensin II was unidirectionally conjugated to its carrier protein. Antisera with predominantly amino terminal specificity were produced when guinea-pigs were immunized with angiotensin II conjugated via its carboxy terminus to thyroglobulin. When angiotensin II was coupled via its amino terminus, carboxy terminal-specific antisera were obtained. The same conjugates failed to provide antisera with the corresponding specificities when rabbits were used instead of guinea-pigs. This work demonstrates that the peptide-protein coupling strategy may affect the specificity of the resulting antiserum.
J
J
Nussberger
G
G
Matsueda
R N
RN
Re
E
E
Haber
D000804:Angiotensin II / Q000276:immunology*; D000818:Animals; D000918:Antibody Specificity; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D007106:Immune Sera / Q000276:immunology; D013954:Thyroglobulin / Q000276:immunology
D000939:Epitopes; D007106:Immune Sera; D000804:Angiotensin II; D013954:Thyroglobulin