Entry into S phase is inhibited in human fibroblasts by rat liver poly(A)+RNA.
Initiation of DNA synthesis was inhibited in human fibroblasts following microinjection with poly(A)+RNA derived from normal rat liver. After sucrose gradient sedimentation of the RNA, the inhibitory activity was found to be limited to two adjacent fractions. Dilution experiments suggest a minimum abundance level of 0.015% for this mRNA(s). Studies on the kinetics of this inhibition indicate a reversible inhibition with a duration of approx. 10 h.
C K
CK
Lumpkin
J K
JK
McClung
J R
JR
Smith
D000818:Animals; D002499:Centrifugation, Density Gradient; D004261:DNA Replication / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D007399:Interphase / Q000187:drug effects*; D007700:Kinetics; D008099:Liver / Q000032:analysis*; D008845:Microinjections; D011061:Poly A / Q000494:pharmacology*; D012313:RNA / Q000494:pharmacology*; D012333:RNA, Messenger; D051381:Rats
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA