[Immunochemical study of alpha and beta forms of NAD-kinase from the rabbit liver].
As shown in immunochemical experiments alpha- and beta-forms of NAD-kinase from rabbit liver tissue consisted of oligomers involving subunits with molecular mass of 33,000 and 62,000, respectively. Both oligomers of the alpha-type with Mr 180,000 and 150,000 and of the beta-type with Mr 280,000 precipitated with the antibodies produced against the alpha-enzyme of Mr 180,000. Use of immunosorbents produced after immobilization of the alpha- and beta-forms of NAD-kinase on odiphile enabled to estimate the ratio of antigenic determinants in oligomers of both NAD-kinase forms. Subunit of the enzyme beta-form carried 3-fold more determinants than the subunits of the alpha-form. The isozyme nature of the alpha- and beta-forms of NAD-kinase is discussed.
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Voronina
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Bulygina
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Telepneva
D000818:Animals; D002645:Chickens / Q000276:immunology; D000939:Epitopes / Q000032:analysis; D005779:Immunodiffusion; D007163:Immunosorbent Techniques; D008099:Liver / Q000201:enzymology*; D046911:Macromolecular Substances; D010770:Phosphotransferases / Q000032:analysis* / Q000276:immunology; D017853:Phosphotransferases (Alcohol Group Acceptor); D011817:Rabbits
D000939:Epitopes; D046911:Macromolecular Substances; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C021844:NAD kinase
Immunokhimicheskoe izuchenie alpha- i beta-form NAD-kinazy iz pecheni krolika.