A simple and rapid method of quantitative analysis of phosphoamino acids by high-performance liquid chromatography.
A high-performance liquid chromatographic system for the separation of nonradiolabeled phosphoamino acids and orthophosphate by ion-pair reverse-phase chromatography has been developed. By the use of low-ionic-strength phthalate buffers at pH 6.3, the phosphoamino acids can be visualized by virtue of this uv-active eluant. The technique is sensitive to 200 pmol of phosphoamino acid and has been shown to be directly applicable to the analysis of isolated phosphoproteins.
N
N
Morrice
A
A
Aitken
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D010710:Phosphates / Q000032:analysis; D010750:Phosphoproteins / Q000032:analysis; D010766:Phosphorylation; D010768:Phosphoserine / Q000032:analysis*; D010769:Phosphothreonine / Q000032:analysis*; D019000:Phosphotyrosine; D012694:Serine / Q000031:analogs & derivatives*; D013912:Threonine / Q000031:analogs & derivatives*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis
D010710:Phosphates; D010750:Phosphoproteins; D010769:Phosphothreonine; D010768:Phosphoserine; D019000:Phosphotyrosine; D013912:Threonine; D014443:Tyrosine; D012694:Serine