A human prostatic growth factor (hPGF): partial purification and characterization.
A growth factor capable of stimulating DNA synthesis of BALB/3T3 cells was purified about 1,000-fold from the cytosol of human benign hypertrophic prostates by heparin-Sepharose chromatography; the growth factor bound to the column in the presence of 0.5 M NaCl was eluted with 1.5-1.7 M NaCl. Its molecular weight and isoelectric point were estimated to be 11,000-13,000 and 10.5, respectively. It was sensitive to heat- and acid-treatments but resistant to disulfide-reducing agent. The final preparation was able to stimulate DNA synthesis at 10 ng/ml. The degree of stimulation was dependent on serum concentration in the assay system; the degree of maximum stimulation increased about 5 times as serum concentration increased from 0.2 to 2%.
Nishi
N
N
Matuo
Y
Y
Muguruma
Y
Y
Yoshitake
Y
Y
Nishikawa
K
K
Wada
F
F
D003600:Cytosol / Q000032:analysis; D004815:Epidermal Growth Factor / Q000032:analysis; D006133:Growth Substances / Q000302:isolation & purification*; D006801:Humans; D007526:Isoelectric Point; D008297:Male; D008970:Molecular Weight; D011467:Prostate / Q000032:analysis*; D011470:Prostatic Hyperplasia / Q000378:metabolism
D006133:Growth Substances; D004815:Epidermal Growth Factor