Effect of sodium butyrate on the complexity and the translation activity of hepatoma tissue-cultured cell RNAs.
We have studied the effect of sodium butyrate on RNA populations and on the in vitro translation pattern of RNA from Hepatoma Tissue-Cultured (HTC) cells. Since sodium butyrate inhibits cell growth we have used stationary cells as control. Molecular hybridization study of cDNA with polyadenylated RNAs shows that sodium butyrate induces the formation of additional 15% new RNA sequences, essentially in the class of abundant sequences and of sequences of intermediary abundance. This drug also decreases 5-10 times the frequency of the sequences of intermediary abundance. One- and two-dimensional polyacrylamide gel electrophoreses of the translational products of RNAs show that sodium butyrate induces or strongly increases the synthesis of 13 polypeptides and decreases the synthesis of 6 polypeptides, which is consistent with the effect of sodium butyrate on RNA populations and could result from the effect of this drug at both transcriptional and early post-transcriptional steps. Electrophoretic analysis of the in vitro translation products of RNAs from cells submitted to sodium butyrate and transferred to a normal medium for various lengths of time shows that most of the polypeptides return to a normal pattern after a certain length of time which varies according to the polypeptide.
Raymondjean
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M
Tichonicky
L
L
Kruh
J
J
D000818:Animals; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects*; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger; D012334:RNA, Neoplasm / Q000378:metabolism*
D002087:Butyrates; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D020148:Butyric Acid; D011061:Poly A; D012313:RNA