Reverse transcriptase of rat liver associated with the endogenous retrovirus related to the mouse intracisternal A-particles.
Reverse transcriptase activity was found in rat liver enclosed in virus-like particles. Through hybridization with DNA probes of A- and C-type retroviruses and with the help of electron microscopy the virus-like particles have been identified as endogenous retroviruses related to the mouse intracisternal A-particles. Blot hybridization revealed the provirus DNA in the rat genome. The enzyme was isolated from the virus-like particles, purified and characterized. The main properties of the enzyme resemble those of the mammalian retrovirus reverse transcriptase.
Salganik
R I
RI
Tomsons
V P
VP
Pyrinova
G B
GB
Korokhov
N P
NP
Kiseleva
E V
EV
Khristolyubova
N B
NB
D000818:Animals; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004247:DNA / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D008099:Liver / Q000201:enzymology* / Q000382:microbiology; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000235:genetics; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D012367:RNA, Viral / Q000235:genetics; D012194:RNA-Directed DNA Polymerase / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012190:Retroviridae / Q000201:enzymology* / Q000235:genetics; D013379:Substrate Specificity
D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; D011061:Poly A; D012313:RNA; D004247:DNA; D012194:RNA-Directed DNA Polymerase