Differential staining of dead and dying embryonic cells with a simple new technique.
A new staining technique with toluidine blue and safranin is described which yields satisfactory results in histological sections of chick embryo tissue fixed in a mixture of glutaraldehyde and formaldehyde and embedded in Spurr's resin. In zones of embryonic cell death, this technique allows the blue-staining of dying cell pyknotic nuclei to be clearly distinguished from red-staining healthy cells. The possible factors underlying this differential staining of pyknotic nuclei are discussed.
G
G
Martín-Partido
I S
IS
Alvarez
L
L
Rodríguez-Gallardo
J
J
Navascués
D000818:Animals; D002470:Cell Survival; D002642:Chick Embryo / Q000166:cytology*; D004900:Erythroblasts / Q000502:physiology; D004905:Erythrocyte Aging; D008853:Microscopy / Q000379:methods*; D010619:Phenazines; D013194:Staining and Labeling / Q000379:methods*; D014048:Tolonium Chloride
D010619:Phenazines; D014048:Tolonium Chloride; C009195:safranine T