Cytochemical characterization of leukemic cells from 20 dogs.
The hematopoietic cells in blood and/or bone marrow from 20 leukemic dogs and 22 control dogs were characterized using a battery of cytochemical stains. The results of cytochemical staining led to modification of the diagnoses based on clinical, hematologic and histologic findings in seven (35%) of the leukemias. Sudan black B and chloroacetate esterase served as granulocytic markers in both the control and leukemic groups. Peroxidase activity was present in the granulocytes and monocytes of control animals but not the blasts of leukemic dogs. Alkaline phosphatase-positive staining of granulocytic precursors was a consistent finding in granulocytic and myelomonocytic leukemia, and alkaline phosphatase-positive lymphoblasts were seen in 38% of lymphocytic leukemias. Diffuse alpha naphthyl butyrate esterase-positive staining marked monocytes in both control and leukemic dogs. Cytochemical staining was found to be a valuable diagnostic aid in the classification of leukemias in the dog.
Facklam
N R
NR
Kociba
G J
GJ
D000135:Acid Phosphatase; D000469:Alkaline Phosphatase; D000818:Animals; D001391:Azo Compounds; D001853:Bone Marrow / Q000473:pathology; D002265:Carboxylic Ester Hydrolases; D004283:Dog Diseases / Q000175:diagnosis / Q000473:pathology*; D004285:Dogs; D006412:Hematopoietic Stem Cells / Q000032:analysis; D006651:Histocytochemistry; D007527:Isoenzymes; D007938:Leukemia / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D009281:Naphthalenes; D010503:Periodic Acid-Schiff Reaction; D009195:Peroxidase; D010544:Peroxidases; D013194:Staining and Labeling / Q000379:methods
D001391:Azo Compounds; D007527:Isoenzymes; D009281:Naphthalenes; C016118:Sudan Black B; D010544:Peroxidases; D009195:Peroxidase; D002265:Carboxylic Ester Hydrolases; C019873:chloroacetate esterase; C021855:naphthylbutyrate esterase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase