Comparison of mutagenicity and theoretical reactivity of 2,4-dinitrobenzaldehyde and 2,6-dinitrobenzaldehyde in bacterial mutation assay and molecular orbital method.
The mutagenicities and theoretical reactivity indices of 2,4-dinitrobenzaldehyde (2,4-DNBAl) and 2,6-dinitrobenzaldehyde (2,6-DNBAl) were investigated using Salmonella typhimurium strains TA98, TA98NR, TA98/1,8-DNP6, and TA100, TA100NR and TA100/1,8-DNP6, by means of the modified intermediate neglect of differential overlap/3 (MINDO)/3) method. The mutagenic activities of 2,4-DNBAl in TA98NR and TA98/1,8-DNP6 were lower than in TA98, whereas the activity in TA100NR was higher than in TA100 and TA100/1,8-DNP6. The mutagenic activity of 2,6-DNBAl in TA100 and that in TA100 and TA100/1,8-DNP6 decreased. These results suggest that the mutagenicities of 2,4-DNBAl and 2,6-DNBAl are dependent either on the microbial nitroreduction and subsequent acetylation or the presence of an aldehyde group. Among the reactivity indices examined, the frontier electron density values were correlated to the mutagenicities of 2,4-DNBAl and 2,6-DNBAl in TA100, TA100NR and TA100/1,8-DNP6 and the values of energy of the lowest unoccupied molecular orbit were correlated to the mutagenicities of several substituted dinitrobenzenes.
Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
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Sayama
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Mori
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Shinoda
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Kozuka
D000107:Acetylation; D000818:Animals; D001547:Benzaldehydes / Q000378:metabolism / Q000633:toxicity*; D001711:Biotransformation; D008862:Microsomes, Liver / Q000378:metabolism; D015394:Molecular Structure; D009152:Mutagenicity Tests; D009153:Mutagens; D009601:Nitroreductases / Q000378:metabolism; D051381:Rats; D012486:Salmonella typhimurium / Q000235:genetics / Q000378:metabolism; D013329:Structure-Activity Relationship
D001547:Benzaldehydes; D009153:Mutagens; C060875:2,6-dinitrobenzaldehyde; D009601:Nitroreductases; C053732:2,4-dinitrobenzaldehyde