A specific enzyme-linked immunosorbent assay (ELISA) procedure for detection of heterophile Hanganutziu and Deicher (HD) antibodies.
A specific, relatively sensitive, quantitative and standardized enzyme-linked immunosorbent assay (ELISA) procedure was developed for the detection of heterophile Hanganutziu and Deicher (HD) antibodies which are occasionally elevated in pathologic human sera. The HD antigen-active molecule used for the assay was a ganglioside (N-glycolylneuraminyllactosylceramide, abbreviated as NeuGc-LacCer) previously purified from horse erythrocyte membranes. The test used antigen-coated plastic microtiter plates and anti-human immunoglobulin G (IgG, Fab fragment) conjugated with alkaline phosphatase. Fifty-four normal human sera gave ELISA values ranging from -2 to 2%. Random sera from hospitalized patients were first screened by the horse erythrocyte hemagglutination (HA) test, whereby 5.7% (76 cases) gave abnormal HA titers of 128-4096 compared to titers in normal sera equal to or less than 64. Ninety-seven % of the patients' sera gave abnormal ELISA values (3-200%). They were classified into 3 groups: cancer (42 cases), infection (10 cases), and others (24 cases). The potential value of this ELISA method is discussed.
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JC
Mukuria
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M
Naiki
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M
Hashimoto
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S
Kato
D000910:Antibodies, Heterophile / Q000302:isolation & purification*; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D004910:Erythrocyte Membrane / Q000276:immunology*; D005732:Gangliosides / Q000097:blood / Q000276:immunology; D006023:Glycoproteins / Q000097:blood / Q000276:immunology; D006801:Humans; D007239:Infections / Q000276:immunology; D009369:Neoplasms / Q000276:immunology; D009438:Neuraminic Acids / Q000276:immunology*
D000910:Antibodies, Heterophile; D000939:Epitopes; D005732:Gangliosides; D006023:Glycoproteins; D009438:Neuraminic Acids; C032592:N-glycolylneuraminic acid