Characterization of chromosome replication during S-phase with bromodeoxyuridine labelling in Chinese hamster ovary and HeLa cells.
Chinese hamster ovary (CHO) and HeLa cells were successively pulse labelled at 1-h intervals after the cultures were synchronized at the end of G1 (monitored by flow cytometry). The metaphases analysed afterwards showed R-type replication patterns after 1-h pulses during the early S-phase (SE; from h 1-5 after release) and replication of G- and C-bands in late S-phase (SL: from h 6-8 after release). The transition from SE to SL is abrupt, constituting a sudden switch of replication between different types of chromatin as has been described for human lymphocytes. The differences between these two cell lines and earlier results reported on a V79 Chinese hamster cell line and on normal diploid human and Chinese hamster fibroblasts are discussed.
W
W
Vogel
S
S
Boldin
A
A
Reisacher
G
G
Speit
D000818:Animals; D001973:Bromodeoxyuridine; D002460:Cell Line; D002875:Chromosomes / Q000648:ultrastructure*; D002877:Chromosomes, Human / Q000648:ultrastructure*; D006224:Cricetinae; D003412:Cricetulus; D004261:DNA Replication; D005260:Female; D006367:HeLa Cells / Q000166:cytology; D006801:Humans; D007399:Interphase; D008677:Metaphase; D010053:Ovary; D013194:Staining and Labeling
D001973:Bromodeoxyuridine