Assignment of the gene for human DNA polymerase alpha to the X chromosome.
We have applied an assay based on a monoclonal antibody that discriminates the activity of human DNA polymerase alpha in rodent-human somatic cell hybrid clones to identify a single genetic locus that is both necessary and sufficient for the expression of DNA polymerase alpha. We have mapped this locus to the short arm of the human X chromosome, near the junction of bands Xp21.3 and Xp22.1, and demonstrated that it is not expressed from an inactive X chromosome.
T S
TS
Wang
B E
BE
Pearson
H A
HA
Suomalainen
T
T
Mohandas
L J
LJ
Shapiro
J
J
Schröder
D
D
Korn
D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D002874:Chromosome Mapping; D002999:Clone Cells; D004257:DNA Polymerase II / Q000235:genetics*; D000939:Epitopes / Q000032:analysis; D005260:Female; D005796:Genes; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology; D007041:Hypoxanthine Phosphoribosyltransferase / Q000172:deficiency; D008214:Lymphocytes / Q000201:enzymology; D051379:Mice; D013045:Species Specificity; D014960:X Chromosome
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007041:Hypoxanthine Phosphoribosyltransferase; D004257:DNA Polymerase II