Insulin-specific T cell hybridomas derived from (H-2b x H-2k)F1 mice preferably employ F1-unique restriction elements for antigen recognition.
T cell hybridomas of (B10 X B10.BR)F1 genotype with reactivity to bovine insulin (BI) were established to analyze the restriction and antigen fine specificity of (H-2b X H-2k)F1 T cells towards BI. Our data indicate a focusing of the response on two epitopes on the insulin molecule, the A chain loop determinant comprising amino acids A8 and A10, as well as the glutamic acid residue in position 4 of the A chain. Both were recognized either separately or in conjunction. Unexpectedly, the T cell hybridomas exhibited a marked preference for recognizing insulin in the context of F1-unique restriction elements of Ab alpha Ak beta type rather than parental high-responder I-Ab molecules. Analysis of the response of primed lymph node T cells of (B10 X B10.BR)F1 mice towards BI corroborated the finding of a preponderant corecognition of F1-unique I-A molecules.
A B
AB
Reske-Kunz
E
E
Rüde
D000818:Animals; D002478:Cells, Cultured; D000939:Epitopes; D006183:H-2 Antigens / Q000276:immunology; D006579:Heterozygote; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology*; D007328:Insulin / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008815:Mice, Inbred Strains; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000939:Epitopes; D006183:H-2 Antigens; D000949:Histocompatibility Antigens Class II; D007328:Insulin; D011948:Receptors, Antigen, T-Cell