Hydrocortisone inhibits phorbol ester stimulated release of histamine and arachidonic acid from rat mast cells.
Purified rat peritoneal mast cells were incubated overnight with or without hydrocortisone (3 X 10(-6) M) and then stimulated with anti-IgE, somatostatin or a phorbol ester-ionophore combination, i.e., 12-O-tetradecanoyl-phorbol-13-acetate and A23187. The release of both histamine and [1-14C]arachidonic acid and its metabolites was determined. Hydrocortisone treatment markedly inhibited both anti-IgE and TPA-A23187 stimulated release, but not release stimulated by somatostatin. These results suggest that anti-inflammatory steroids may alter histamine release through an action involving the activation of the phosphatidylserine-calcium dependent protein kinase or its substrates.
Heiman
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D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects*; D006854:Hydrocortisone / Q000494:pharmacology*; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D010704:Phorbols / Q000494:pharmacology*; D051381:Rats; D013004:Somatostatin / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D001095:Arachidonic Acids; D010704:Phorbols; D016718:Arachidonic Acid; D000001:Calcimycin; D013004:Somatostatin; D013755:Tetradecanoylphorbol Acetate; D006854:Hydrocortisone