Bovine T lymphocyte response to bovine herpesvirus-1: cell phenotypes, viral recognition and acid-labile interferon production.
The phenotype of bovine cells that proliferate to bovine herpesvirus (BHV-1) were identified by peanut agglutinin (PNA) and monoclonal antibodies with specificity for Pan T cells (B29), a T cell subset (B24), and an MHC class II (Ia-like) antigen (R-1). PNA+ cells but not PNA- cells separated by fluorescent activated cell sorting responded to BHV-1. Virally-activated T cells expressed MHC class II antigens, and possibly antigen specific receptors for virus. Binding of radiolabeled virus increased six-fold beyond the expected value for activated cells suggesting specificity of the lymphocytes for the virus. Finally, cells that responded to BHV-1 produced high levels of acid sensitive interferon. Taken together these results characterize the phenotypes of bovine lymphocytes that interact with BHV-1.
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002417:Cattle; D003602:Cytotoxicity, Immunologic; D007242:Herpesvirus 1, Bovine / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007372:Interferons / Q000096:biosynthesis*; D037102:Lectins / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D019887:Peanut Agglutinin; D010641:Phenotype; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; D037102:Lectins; D019887:Peanut Agglutinin; D007372:Interferons