[Heat resistance of Cladosporium isolated from laboratory animal facilities].
Heat resistance tests for the saprophyte, Cladosporium, isolated from laboratory animal facilities were carried out. In testing the effects of moderate and high temperature conditions, C. sphaerospermum (C. s) and C. cladosporioides (C. c) were found to grow on media in temperatures less than 32 degrees C, but did not in temperature of 35 degrees C and over. The colony diameter of Cladosporium became smaller as temperature increased. The death time of C. s treated with moist heat was within 12 min at 48 degrees C and that of C. c was within 26 min at 43 degrees C. Both Cladosporium species could not survive for more than 1 min at 55 degrees C. On the other hand, Cladosporium treated with dry heat could not survive more than 69-12 min (C. s) and 39-9.5 min (C. c) at 70-100 degrees C. From these results, it can be seen that Cladosporium was definitely sensitive to heat treatment, and the authors assume that heat is a means of prevention in laboratory animal facilities.
Hatano Research Institute, Food and Drug Safety Center, Kanagawa, Japan.
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Kawai
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Takatori
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Ohtaki
D000822:Animal Husbandry; D000818:Animals; D000830:Animals, Laboratory / Q000382:microbiology*; D002963:Cladosporium / Q000254:growth & development* / Q000302:isolation & purification; D006358:Hot Temperature; D003904:Mitosporic Fungi / Q000254:growth & development*