Use of recombinant hepatitis delta antigen in diagnostic assays for HDV antibody.
The gene encoding the hepatitis delta virus (HDV) structural antigen (HD Ag) was inserted into a Rous sarcoma virus expression vector and the recombinant plasmid used to direct the synthesis of recombinant HD Ag (rHD Ag) in a continuous hepatoma cell line. A competitive radioimmunoassay for serum antibody to HDV using rHD Ag was developed and was found to be equally suitable for diagnostic purposes to a radioimmunoassay using infected liver-derived HD Ag. Similarly, rHD Ag was shown to be serologically equivalent to liver-derived HD Ag within the limit of the blocking titrations performed. The rHD Ag-positive cell line was also used in an indirect immunofluorescence assay to detect anti-HD. Similar titres of anti-HD were detected by both radioimmunoassay and immunofluorescence and identical samples were positive for anti-HD by either assay. In a sample of prison inmates with high prevalence of both HBV and HDV, anti-HD was confined almost exclusively to those with persistent HBV infection and not to those in whom HBV infection had cleared. The availability of rHD Ag will permit wider development of diagnostic anti-HD assays, and the use of two such assays is presented in this study.
Division of Medical Virology, Institute of Medical and Veterinary Science, Adelaide, Australia.
E J
EJ
Gowans
T B
TB
Macnaughton
L
L
Mickan
A R
AR
Jilbert
C J
CJ
Burrell
D000956:Antigens, Viral / Q000276:immunology*; D001667:Binding, Competitive; D005455:Fluorescent Antibody Technique; D006508:Hepatitis Antibodies / Q000032:analysis; D003699:Hepatitis D / Q000175:diagnosis*; D003698:Hepatitis Delta Virus / Q000276:immunology*; D035923:Hepatitis delta Antigens; D006801:Humans; D015995:Prevalence; D011863:Radioimmunoassay; D011994:Recombinant Proteins / Q000276:immunology; D012307:Risk Factors; D012680:Sensitivity and Specificity
D000956:Antigens, Viral; D006508:Hepatitis Antibodies; D035923:Hepatitis delta Antigens; D011994:Recombinant Proteins; C442244:hepatitis delta virus large antigen