Smooth muscle actin expression during P19 embryonal carcinoma differentiation in cell culture.
P19 embryonal carcinoma (EC) cells can be induced in vitro to differentiate into cells resembling those normally formed in the embryo. Among these cell types is one whose morphology is fibroblast-like. Using indirect immunofluorescence and Western blot analysis with antibodies directed against various isoforms of actin, many of these fibroblast-like cells were found to express smooth muscle actin isoforms. Northern blot analysis of RNA indicated the presence of a smooth muscle-specific isoform of myosin heavy-chain mRNA in immortal lines of these fibroblast-like cells. These results suggest that these fibroblast-like cells resemble fetal myofibroblastic or myoepithelial cells, which have a wide distribution during embryonic development.
Department of Medicine, University of Ottawa, Ontario, Canada.
M A
MA
Rudnicki
N M
NM
Sawtell
K R
KR
Reuhl
R
R
Berg
J C
JC
Craig
K
K
Jardine
J L
JL
Lessard
M W
MW
McBurney
D000199:Actins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000378:metabolism* / Q000473:pathology; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D009130:Muscle, Smooth / Q000032:analysis; D009374:Neoplasms, Experimental / Q000378:metabolism / Q000473:pathology*; D013724:Teratoma / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology
D000199:Actins