Invasion-related antigens of Campylobacter jejuni.
A HEp-2 cell culture model was used to investigate the antigens required for epithelial cell penetration by Campylobacter jejuni. Penetration of HEp-2 epithelial cells by C. jejuni was significantly inhibited (P less than .05) with C. jejuni lysate and a monoclonal antibody (MAb 1B4) in competitive inhibition assays. Immunogold electron microscopy revealed that MAb 1B4 bound to the flagella and cell surface of low-passage (invasive) C. jejuni M 96, whereas only the flagella of high-passage (noninvasive) C. jejuni M 96 were labeled. Western blot analysis revealed that MAb 1B4 identified an epitope on antigens of 64-44 kDa in lysates prepared from invasive and noninvasive isolates. In addition, antigens of 42-38 kDa were recognized in lysates prepared from only invasive C. jejuni strains. Proteinase K and sodium meta-periodate chemical treatment of C. jejuni M 96 lysate changed the mobility of antigens recognized by MAb 1B4. The increase in mobility demonstrated a decrease in size of molecules and suggested that antigens required for HEp-2 cell invasion by Campylobacter species may be glycoprotein in nature.
Konkel
M E
ME
Department of Veterinary Science, University of Arizona, Tucson 85721.
Babakhani
F
F
Joens
L A
LA
D000942:Antigens, Bacterial / Q000032:analysis*; D001422:Bacterial Adhesion; D015153:Blotting, Western; D002168:Campylobacter fetus / Q000276:immunology* / Q000472:pathogenicity / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms; D008854:Microscopy, Electron; D014407:Tumor Cells, Cultured; D014774:Virulence
D000942:Antigens, Bacterial