Disassociation of bactericidal and fungistatic activities from the oxidative burst of avian macrophages.
Avian peritoneal exudate macrophages, when exposed to phagocytic stimuli, produced an appreciable oxidative burst as measured by production of chemiluminescence, superoxide anion, and hydrogen peroxide. Metabolic inhibitors of the oxidative burst and scavengers of oxygen radicals clearly inhibited macrophage chemiluminescence, but had no significant effect on macrophage bactericidal activity against Escherichia coli or fungistatic activity against Candida tropicalis. Therefore, avian macrophages were capable of oxygen-independent bactericidal and fungistatic activities.
Department of Veterinary Pathology, College of Veterinary Medicine, University of Georgia, Athens 30602.
B G
BG
Harmon
J R
JR
Glisson
D000818:Animals; D002175:Candida; D002645:Chickens; D004926:Escherichia coli / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000032:analysis; D008163:Luminescent Measurements; D008264:Macrophages / Q000378:metabolism* / Q000502:physiology; D010084:Oxidation-Reduction; D010537:Peritoneum; D010587:Phagocytosis; D013482:Superoxide Dismutase
D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase