[Interaction of EcoRII restriction and modification enzymes with synthetic DNA fragments. Determination of the size of EcoRII binding site].
EcoRII restriction endonuclease binding site has been determined on the basis of comparison of the binding parameters of the enzyme with synthetic DNA-duplexes of concatemer type containing a different number of EcoRII recognition sites. It has been shown that it consists of 21 +/- 1 base pairs.
M V
MV
Vinogradova
E S
ES
Gromova
V G
VG
Kosykh
Ia I
IaI
Bur'ianov
Z A
ZA
Shabarova
D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes / Q000378:metabolism; D013379:Substrate Specificity
D009692:Nucleic Acid Heteroduplexes; D004247:DNA; C046545:CCWGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific
Vzaimodeĭstvie fermentov restriktsii i modifikatsii EcoRII s sinteticheskimi fragmentami DNK. XI. Opredelenie velichiny mesta posadki éndonukleazy EcoRII na substrat.