Cultivation of macrophages derived from human malignant effusions.
Macrophages, isolated from malignant effusions, were cultured in vitro in media containing GCT/CM as a source of granulocyte-macrophage (GM) colony-stimulating factor (CSF). Cells were periodically examined for macrophage-specific cell surface antigens and functional activity. The number of macrophages increased sixfold during 15 days in culture. Autoradiographic studies confirmed that macrophages were incorporating 3H-Tdr. Macrophage-specific cell surface markers were retained up to five weeks in culture. Cells were also able to express macrophage-specific functions including phagocytosis and secretion of lysozyme. The results indicate that human tumor-derived macrophages may be expanded in short-term culture in the presence of GM CSF.
A W
AW
Hamburger
C P
CP
White
F E
FE
Dunn
D000954:Antigens, Surface / Q000032:analysis; D001202:Ascitic Fluid / Q000473:pathology*; D002455:Cell Division; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors; D003470:Culture Media; D000939:Epitopes; D005260:Female; D005870:Giant Cell Tumors / Q000473:pathology; D006801:Humans; D008264:Macrophages / Q000166:cytology* / Q000276:immunology; D009369:Neoplasms / Q000473:pathology*; D010996:Pleural Effusion / Q000473:pathology*
D000954:Antigens, Surface; D003115:Colony-Stimulating Factors; D003470:Culture Media; D000939:Epitopes