The 3' end of large ribosomal subunit RNA from mosquito mitochondria: homogeneity of transcribed moieties.
3' Terminal sequences of mosquito mitochondrial 16 S ribosomal RNA, which is post-transcriptionally polyadenylated, have been examined with the aim of determining the degree of homogeneity of transcribed moieties. 3' End-labeled samples were subjected to oligonucleotide fingerprint analysis and to ladder gel analysis after primary and secondary nuclease digestion; and products of reverse transcriptase reactions were characterized using 16 S RNA as template and selected oligodeoxynucleotides as primers. The results indicated a remarkable degree of homogeneity compared to homologous mammalian mitochondrial systems, and suggested differences in modes of expression of insect, versus mammalian, mitochondrial genomes.
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Dubin
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HsuChen
D000330:Aedes / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000032:analysis / Q000235:genetics; D008928:Mitochondria / Q000032:analysis*; D009838:Oligodeoxyribonucleotides / Q000032:analysis / Q000235:genetics; D012335:RNA, Ribosomal / Q000032:analysis* / Q000235:genetics; D012194:RNA-Directed DNA Polymerase; D013698:Templates, Genetic; D014158:Transcription, Genetic
D009838:Oligodeoxyribonucleotides; D012335:RNA, Ribosomal; D004247:DNA; D012194:RNA-Directed DNA Polymerase