Cellular retinoic acid-binding protein and its relationship to the biological activity of four synthetic retinoids in hamster tracheal organ culture.
The mechanism of action of retinoid in reversing keratinization in hamster trachea is yet unknown. The purpose of this study was to determine if cellular retinoic acid binding protein (CRABP) is present in tracheal epithelium following incubation in serum-free, vitamin A-deficient culture medium for 10 days, and if the effectiveness of a retinoid in reversing keratinization in organ culture is correlated with its ability to compete for CRABP sites. The cytosol prepared from tracheal cultures contained CRABP at a concentration of 2.61 pmoles per mg protein. Of the four retinoids with carboxyl end group selected for the study, two of the biological active retinoids competed for the CRABP sites. However, no correlation was observed between the biological activity of the inactive retinoids and their ability to associate with the CRABP sites. These results indicate that even though the action of retinoid may be mediated by retinoid binding protein, it cannot be used as a sole predicator of retinoid response in hamster trachea.
Mehta
R G
RG
Schiff
L J
LJ
Moore
S J
SJ
Buckley
A M
AM
Dawson
M I
MI
D000818:Animals; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D006224:Cricetinae; D003600:Cytosol / Q000378:metabolism; D007633:Keratins / Q000378:metabolism; D009924:Organ Culture Techniques; D018168:Receptors, Retinoic Acid; D012176:Retinoids / Q000378:metabolism / Q000494:pharmacology*; D014132:Trachea / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
D002352:Carrier Proteins; D018168:Receptors, Retinoic Acid; D012176:Retinoids; D007633:Keratins