The effect of 2,5-hexanedione on the induction of ornithine decarboxylase in the dorsal root ganglion of the rat.
Rat dorsal root ganglia respond to sciatic nerve injury with an increase in the activity of the enzyme ornithine decarboxylase (ODC). The increase is impaired under certain conditions (e.g. diabetes, Vinca alkaloid treatment) where retrograde axonal transport is reduced. The purpose of the experiments was to determine if the neurotoxin 2,5-hexanedione, also known to interfere with retrograde axonal transport, similarly affected ODC induction. Rats were treated with 2,5-hexanedione i.p. to a cumulative dose of 6 and 8 g/kg. One sciatic nerve was crushed under anaesthesia and 24 h later the dorsal root ganglia were removed and assayed for ODC activity by a radioenzymatic method. The ratio of ODC activity of 1.57 +/- 0.58 (crushed side over control side) was reduced to 1.02 +/- 0.41 1.08 +/- 0.39 after 2,5-hexanedione at 6 g and 8 g/kg, respectively. The enzyme was not inhibited by addition of 2,5-hexanedione in vitro. The results confirm the role of retrograde axonal transport in nerve cell responses to injury and are consistent with the effects of 2,5-hexanedione on nerve regeneration.
Department of Pharmacology and Therapeutics, University of Liverpool, U.K.
O T
OT
Myall
S L
SL
Allen
W G
WG
McLean
D000818:Animals; D001369:Axons / Q000378:metabolism; D005260:Female; D005727:Ganglia, Spinal / Q000378:metabolism*; D006588:Hexanones / Q000494:pharmacology*; D007659:Ketones / Q000494:pharmacology*; D009409:Nerve Crush; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D006588:Hexanones; D007659:Ketones; C011269:2,5-hexanedione; D009955:Ornithine Decarboxylase