Evidence for androgen receptors in sexually dimorphic perineal muscles of neonatal male rats. Absence of androgen accumulation by the perineal motoneurons.
During development, survival of the sexually dimorphic spinal nucleus of the bulbocavernosus (SNB) and its target perineal muscles, the bulbocavernosus (BC) and the levator ani (LA) is androgen-dependent. To define androgen's site of action in masculinizing SNB system structures, we examined whether or not androgen receptors are present in SNB motoneurons and/or BC/LA muscles of neonatal male rats. Using a receptor binding assay, we have identified androgen-binding factors in the neonatal BC/LA (Bmax = 13.5 fmol/mg protein; Kd = 4.69 nM) for the first time. In contrast, androgen autoradiography provided no evidence that neonatal spinal motoneurons accumulate androgens. These results support the hypothesis that BC/LA muscles are a primary site of androgen action for masculinizing SNB system structures, and that androgen need not interact with SNB motoneurons directly to sexually differentiate them.
Fishman
R B
RB
Department of Psychology, University of California, Berkeley 94720.
Chism
L
L
Firestone
G L
GL
Breedlove
S M
SM
D000375:Aging / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D001345:Autoradiography; D008297:Male; D009046:Motor Neurons / Q000378:metabolism*; D010502:Perineum / Q000294:innervation*; D051381:Rats; D011919:Rats, Inbred Strains; D011944:Receptors, Androgen / Q000378:metabolism*; D012727:Sex Characteristics; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism
D011944:Receptors, Androgen