L-2,4-diaminobutyric acid decarboxylase activity responsible for the formation of 1,3-diaminopropane in Enterobacter aerogenes.
High content of 1,3-diaminopropane (DAP), a normally minor derivative of polyamine metabolism, have been observed in cells of Enterobacter aerogenes. Supplementation of the growth medium with L-2,4-diaminobutyric acid (L-DABA) resulted in increased production of DAP, but not if supplemented with spermidine. On the basis of these observations, the biosynthetic route for DAP was evaluated. It has appeared that this bacterium possesses a novel enzyme activity catalysing the formation of DAP from L-DABA. Lack of the activity for oxidative cleavage of spermidine yielding DAP suggests that the enzyme termed DABA decarboxylase is responsible for the formation of DAP in this bacterium. The enzyme was partially purified 360-fold and some properties were examined. The pH optimum for the activity was 7.75-8.0, and the enzyme showed an absolute requirement for pyridoxal 5'-phosphate with the Km value of 41 microM. The Km value for L-DABA was 0.32 mM, and neither L-2,3-diaminopropionic acid, L-ornithine nor L-lysine showed detectable substrate activity towards the partially purified enzyme. Mg2+ and dithiothreitol greatly activated the enzyme.
Nakao
H
H
Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Takeuchi
K
K
Shinoda
S
S
Yamamoto
S
S
D002262:Carboxy-Lyases / Q000302:isolation & purification / Q000378:metabolism*; D002849:Chromatography, Gas; D003959:Diamines / Q000378:metabolism*; D004229:Dithiothreitol / Q000494:pharmacology; D004754:Enterobacter / Q000201:enzymology*; D004755:Enterobacteriaceae / Q000201:enzymology*; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D011732:Pyridoxal Phosphate / Q000378:metabolism; D013379:Substrate Specificity
D003959:Diamines; D011732:Pyridoxal Phosphate; C009475:trimethylenediamine; C062274:2,4-diaminobutyric acid decarboxylase; D002262:Carboxy-Lyases; D008274:Magnesium; D004229:Dithiothreitol