[Cytotoxic properties of the splenocytes of C3HA mice following treatment with exogenous RNA].
The influence of fractions of exogenous RNA, isolated from spleens of C3HA mice and of rats, both intact (control--cRNA) and immunized with homogenate of normal syngenic, allogenic and xenogenic tissues (immune--immRNA), on the cytotoxic properties of splenocytes of C3HA intact mice was compared in the in vitro cytotoxic experiments. The splenocytes treated with different RNA fractions were used as effector-cells. In vitro cultivated MGXXIIa cells of strain specific C3HA mice hepatoma, and K562 cells of human erythroleukemia, both labeled with 3H-uridine, served as target cells. Thus, it is only the cytoplasmic fraction of immRNA isolated from the spleens of rats immunized with tissue antigens of C3HA mice that induced a more pronounced stimulation of cytotoxic activity of splenocytes.
Aksenova
N N
NN
Golosova
Iu V
IuV
Sleptsova
L A
LA
Fel'
V Ia
VIa
D000818:Animals; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D006801:Humans; D007114:Immunization; D004915:Leukemia, Erythroblastic, Acute / Q000276:immunology; D008114:Liver Neoplasms, Experimental / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H / Q000276:immunology*; D008810:Mice, Inbred C57BL; D012313:RNA / Q000276:immunology*; D051381:Rats; D013154:Spleen / Q000187:drug effects / Q000276:immunology*
D012313:RNA
Issledovanie tsitotoksicheskikh svoĭstv splenotsitov mysheĭ C3HA posle obrabotki ékzogennoĭ RNK.