Accelerated regenerative neurite formation by a neuronal surface epitope reactive with the monoclonal antibody, Leu 7.
A family of glycoproteins sharing an epitope with myelin associated glycoprotein as recognized by the monoclonal antibody Leu 7 (HNK-1) has been found to be present on neurons grown in culture from embryonic chicks and rats. Immunofluorescent staining demonstrates that, in vitro, 100% of the neurons from dorsal root ganglia and spinal cord from 7-8 day chick embryos react with Leu 7. Analysis of in vitro regenerative neurite formation by neurons on substrates enriched with Leu 7 showed accelerated regenerative process formation under limiting conditions. These results indicate that the Leu 7 epitope on neurons is appropriate for substrate adhesion and promotes rapid process extension.
McGarry
R C
RC
Riopelle
R J
RJ
Roder
J C
JC
D000818:Animals; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface; D002478:Cells, Cultured; D002642:Chick Embryo; D000939:Epitopes / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D009185:Myelin Proteins / Q000276:immunology*; D018992:Myelin-Associated Glycoprotein; D009474:Neurons / Q000276:immunology* / Q000502:physiology; D013116:Spinal Cord
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000939:Epitopes; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein