[Time requirements for positive blood cultures with reference to different methods of determining bacteria in blood cultures].
The time required for the first detection of bacteria in 926 blood-cultures has been evaluated for different methods. The microscopical examination of samples after staining with acridine-orange has been found to be a rapid and simple method, which may reduce in 88% of the samples the time required for the processing and thus increase the value of blood-cultures for the treatment of patients with bacteremia significantly. The results demonstrate that a rapid detection of bacteria in blood-cultures by chemical or microscopical methods is an indispensable part of the processing of blood-cultures, although it has been used only in few bacteriological laboratories until now.
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Küchler
D000165:Acridine Orange; D001419:Bacteria / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D001769:Blood / Q000382:microbiology*; D001800:Blood Specimen Collection / Q000379:methods; D006801:Humans; D008856:Microscopy, Fluorescence; D013045:Species Specificity; D013194:Staining and Labeling; D013997:Time Factors
D000165:Acridine Orange
Die Bearbeitungsdauer von positiven Blutkulturen unter Berücksichtigung verschiedener Methoden des Bakteriennachweises in Blutkulturen.