A radioimmunoassay for thymosin alpha 1 that detects the native polypeptide, prothymosin alpha.
A radioimmunoassay, developed for thymosin alpha 1, can also be utilized for the quantitation of the intact native polypeptide, prothymosin alpha, which contains the thymosin alpha 1 sequence at its NH2-terminus (Haritos et al., 1984a). The major epitope was characterized and found to include residues 1-10 at the NH2-terminus of thymosin alpha 1. As little as 5 pmol of prothymosin alpha can be detected in tissue extracts with this radioimmunoassay.
A A
AA
Haritos
B L
BL
Horecker
D000595:Amino Acid Sequence; D003429:Cross Reactions; D000939:Epitopes; D011498:Protein Precursors / Q000032:analysis*; D011863:Radioimmunoassay; D013947:Thymosin / Q000031:analogs & derivatives* / Q000032:analysis*
D000939:Epitopes; D011498:Protein Precursors; C040782:prothymosin alpha; D013947:Thymosin