Chemotherapy of subcutaneous and intracranial human medulloblastoma xenografts in athymic nude mice.
The continuous human medulloblastoma cell line TE-671 was grown as s.c. and intracranial xenografts in athymic nude mice. Tumor-bearing animals were treated with chemotherapeutic agents at the 10% lethal dose; s.c. xenografts were sensitive to melphalan, 1-(2-chloroethyl)-3-(2,6-dioxo-1-piperidyl)-1-nitrosourea, and 5-azacytidine. No consistent response could be demonstrated to 9-beta-D-arabinofuranosyl-2-fluoroadenine 5'-monophosphate, and no response to methylglyoxal bis(guanyl hydrazone), N-trifluoroacetyl adriamycin-14-valerate, or to 1-beta-D-arabinofuranosylcytosine was observed. Melphalan produced a significant (P = less than or equal to 0.007) increase in the median survival of mice bearing intracranial xenografts, whereas no response was seen to 1-(2-chloroethyl)-3-(2,6-dioxo-1-piperidyl)-1-nitrosourea or 5-azacytidine. This model will allow analysis of the chemotherapeutic profile of human medulloblastoma, and provides a means to differentiate cellular sensitivity and resistance from drug access to the intracranial site.
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Friedman
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Schold
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Bigner
D000818:Animals; D001374:Azacitidine / Q000627:therapeutic use / Q000633:toxicity; D001932:Brain Neoplasms / Q000188:drug therapy*; D002460:Cell Line; D003561:Cytarabine / Q000627:therapeutic use / Q000633:toxicity; D004317:Doxorubicin / Q000031:analogs & derivatives / Q000627:therapeutic use / Q000633:toxicity; D006801:Humans; D008527:Medulloblastoma / Q000188:drug therapy*; D008558:Melphalan / Q000627:therapeutic use / Q000633:toxicity; D051379:Mice; D008819:Mice, Nude; D008935:Mitoguazone / Q000627:therapeutic use / Q000633:toxicity; D009368:Neoplasm Transplantation; D009607:Nitrosourea Compounds / Q000627:therapeutic use / Q000633:toxicity; D012878:Skin Neoplasms / Q000188:drug therapy*; D001084:Vidarabine Phosphate / Q000031:analogs & derivatives / Q000627:therapeutic use / Q000633:toxicity
D009607:Nitrosourea Compounds; D003561:Cytarabine; D001084:Vidarabine Phosphate; C042382:fludarabine phosphate; C016163:valrubicin; D004317:Doxorubicin; C018627:1-(2-chloroethyl)-3-(2,6-dioxo-3-piperidinyl)-1-nitrosourea; D001374:Azacitidine; D008935:Mitoguazone; D008558:Melphalan