Presence and ontogeny of enkephalin and substance P in the chick ciliary ganglion.
The avian ciliary ganglion has been reported to contain both enkephalin and substance P in preganglionic terminals. However, extensive biochemical characterization of these antigens has not been completed. Using radioimmunoassays specific for Met5- and for Leu5-enkephalin and for substance P we identified immunoreactive substances in ganglionic extracts that comigrate on HPLC columns with standard Met5- and Leu5-enkephalin and with substance P. The ontogeny of Met5-enkephalin and substance P during embryogenesis was determined in ganglionic extracts and we found that the content of Met5-enkephalin in the ganglion reached a peak at embryonic stage 37 whereas the content of substance P in the ganglion reached its maximum in the adult.
J D
JD
White
J E
JE
Krause
H J
HJ
Karten
J F
JF
McKelvy
D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D004743:Enkephalin, Leucine / Q000032:analysis; D004744:Enkephalin, Methionine / Q000032:analysis; D004745:Enkephalins / Q000032:analysis*; D005726:Ganglia, Parasympathetic / Q000032:analysis / Q000254:growth & development*; D011863:Radioimmunoassay; D013373:Substance P / Q000032:analysis*
D004745:Enkephalins; D013373:Substance P; D004744:Enkephalin, Methionine; D004743:Enkephalin, Leucine