Centrifugal affinity chromatography.
A new technique termed centrifugal affinity chromatography (CAC) is presented in this paper. CAC combines a high flow-rate, created by centrifugal force, with the specificity of affinity chromatography. This technique has been used for the purification of human immunoglobulin G. Furthermore this technique has been used to remove human albumin from serum and the effect of centrifugal force, ionic strength and pH has been studied. A test for determining the percentage of glycosylated hemoglobin in hemolysates has also been developed. This test, employing centrifugal chromatography, is more than three times faster than commonly used gravity flow methods.
Agricultural University, Wageningen, The Netherlands.
R J
RJ
Slingerland
V
V
Adámek
W H
WH
Scouten
D002498:Centrifugation; D002846:Chromatography, Affinity / Q000295:instrumentation*; D006442:Glycated Hemoglobin / Q000302:isolation & purification; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000302:isolation & purification*; D012709:Serum Albumin / Q000302:isolation & purification; D013205:Staphylococcal Protein A
D006442:Glycated Hemoglobin; D007074:Immunoglobulin G; D012709:Serum Albumin; D013205:Staphylococcal Protein A