Complement-mediated lysis of K562 human leukemic cells by antibodies to phosphotyrosine and identification of cell surface proteins phosphorylated on tyrosine.
Intact K562 human leukemic cells showed bright membrane immunofluorescence after staining with monoclonal antibody to O-phosphotyrosine (PTyr). Up to 60% of the cells were lysed with mouse, rabbit, or human antibodies to PTyr by a complement-mediated mechanism. A new method has been developed for identifying proteins that have PTyr residues on the outside of cell membrane, and at least two species of PTyr-containing proteins with the molecular weights of 45,000 and 36,000 were identified as the most probable candidates of the antigens responsible for the membrane fluorescence and cell lysis.
R
R
Ogawa
M
M
Ohtsuka
Y
Y
Watanabe
D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D003165:Complement System Proteins / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D005455:Fluorescent Antibody Technique; D006801:Humans; D007938:Leukemia / Q000276:immunology*; D008565:Membrane Proteins / Q000276:immunology; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000276:immunology*; D010750:Phosphoproteins / Q000276:immunology*; D019000:Phosphotyrosine; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000276:immunology
D000911:Antibodies, Monoclonal; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D010750:Phosphoproteins; D019000:Phosphotyrosine; D014443:Tyrosine; D003165:Complement System Proteins