Effect of pH on the recovery of human alpha-fetoprotein from immunosorbent columns. An improved elution procedure.
A slow decrease in pH was shown to be more efficient in eluting human alpha-fetoprotein bound to Sepharose-immobilized antibodies than a pH shock. With this method, nearly 50% of the antigen absorbed onto the column was recovered in a single peak whereas the yield was 3 times lower in the case of a single elution at pH 2.6. Our result suggests that dissociation of the antigen-antibody complex occurred only within a narrow pH range.
Ferrua
B
B
Masseyeff
R
R
D000937:Antigen-Antibody Reactions; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007122:Immunoelectrophoresis; D007163:Immunosorbent Techniques; D011487:Protein Conformation; D011489:Protein Denaturation; D000509:alpha-Fetoproteins / Q000302:isolation & purification*
D000509:alpha-Fetoproteins