Free fatty-acid uptake by isolated rat hepatocytes.
In isolated rat hepatocytes, the rate of palmitic acid binding and uptake is directly related to the concentration of free fatty acid (FFA) in the medium. After their entry into the cell, FFA are immediately incorporated into cellular phospholipids and triglycerides and no accumulation of free fatty acids can be demonstrated inside the cell. The rate of free fatty-acid uptake remains unchanged after incubation in a 2 mM KCN containing medium, indicating that in the range of fatty-acid concentrations used in this study, this phenomenon does not require energy.
Renaud
G
G
Bouma
M E
ME
Foliot
A
A
Infante
R
R
D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D011190:Potassium Cyanide / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D005230:Fatty Acids, Nonesterified; D010169:Palmitic Acids; D010743:Phospholipids; D019308:Palmitic Acid; D002784:Cholesterol; D011190:Potassium Cyanide