Human IgG subclass-specific rabbit antisera suitable for immunoprecipitation in gel, ELISA and multilayer haemagglutination techniques.
Antisera specific for human IgG subclasses were raised in rabbits by tolerance induction at birth followed by active immunization at 8 weeks of age. This procedure gave better antisera compared to tolerance/immunization of adult grown animals. Many animals gave antisera with subclass-specific precipitating activity without the need to use absorption procedures. However, for sensitive techniques such as ELISA and multilayer haemagglutination it was necessary to absorb the antisera to make them subclass-specific. Subclass specificity in ELISA and haemagglutination was verified by inhibition. We believe this is the first description of a procedure to prepare antisera specific for human IgG subclasses suitable for use in immunoprecipitation, ELISA and haemagglutination techniques.
Michaelsen
T E
TE
Haug
E
E
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D006386:Hemagglutination Tests; D006801:Humans; D007108:Immune Tolerance; D007114:Immunization / Q000379:methods; D005779:Immunodiffusion; D007128:Immunoglobulin Fragments / Q000276:immunology; D007074:Immunoglobulin G / Q000145:classification / Q000276:immunology*; D007147:Immunoglobulin Light Chains / Q000276:immunology; D007163:Immunosorbent Techniques; D009194:Myeloma Proteins / Q000276:immunology; D011817:Rabbits / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G; D007147:Immunoglobulin Light Chains; D009194:Myeloma Proteins