[Isolation of a biologically active poly(A)+RNA from tissues enriched with polysaccharides].
A special technique is developed for isolation of biologically functional poly(A)+RNA from higher plant cells to start with some procedures used to isolate RNA of the eucaryote cells. The technique includes cell disruption with a 4 M guanidine thiocyanate solution, hot phenol extraction of RNA following proteinase K digestion and methoxyethanol treatment to remove polysaccharides. Finally, poly(A)+RNA was purified by oligo(dT)-cellulose chromatography. Using this technique poly(A)+RNA preparations were obtained from French bean embryo axis, maize and sugar beet seedings, potato tubers and seedlings. It is shown that poly(A)+RNA isolated from the plant cells directs protein synthesis in the cell-free system. This RNA may be also used as a matrix for synthesis of cDNA in the reverse transcription reaction.
N N
NN
Domanskiĭ
A P
AP
Galkin
K M
KM
Sytnik
D000818:Animals; D004247:DNA / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D009693:Nucleic Acid Hybridization; D010936:Plant Extracts / Q000032:analysis; D011061:Poly A / Q000302:isolation & purification*; D011134:Polysaccharides / Q000302:isolation & purification*; D012313:RNA / Q000302:isolation & purification*; D011817:Rabbits; D012156:Reticulocytes / Q000032:analysis
D010936:Plant Extracts; D011134:Polysaccharides; D011061:Poly A; D012313:RNA; D004247:DNA
Poluchenie biologicheski aktivnoĭ poli(A)+RNK iz tkaneĭ, obogashchennykh polisakharidami.