[Growth limitations and biosynthesis of gramicidin in Bacillus brevis var. G.-B].
Gramicidin S biosynthesis was studied in Bacillus brevis var. G.-B. during its batch and continuous cultivation when the culture growth was limited with nutrient sources (glycerol, ammonium nitrogen, phosphate), oxygen deficiency and the action of a physical factor (a low temperature). The antibiotic biosynthesis was shown to be induced by a change in the growth rate caused by the action of any factor decelerating the growth. The authors propose a mathematical model for the antibiotic synthesis, biomass accumulation and the utilization of a substrate limiting the growth. The model is based on the age separation of cells. The model is analyzed in terms of optimizing the one-stage continuous cultivation process. The model allows one to calculate optimal conditions of the antibiotic synthesis in the process of one-stage continuous cultivation.
Lykov
V P
VP
Bodnar
I V
IV
Khovrychev
M P
MP
Polin
A N
AN
Fokht
A S
AS
D001407:Bacillus / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D003080:Cold Temperature; D005990:Glycerol / Q000494:pharmacology; D006096:Gramicidin / Q000096:biosynthesis*; D008954:Models, Biological; D009566:Nitrates / Q000494:pharmacology; D010100:Oxygen; D010710:Phosphates / Q000494:pharmacology
D009566:Nitrates; D010710:Phosphates; D006096:Gramicidin; D005990:Glycerol; D010100:Oxygen; C006568:ammonium nitrate
Limitatsiia rosta i biosintez gramitsidina s Bacillus brevis var. G.-B.