Brain motilin-like peptides are localized within the cell nucleus.
The subcellular localization of motilin-like immunoreactive (MLIP) peptides in comparison to vasoactive intestinal polypeptide (VIP) and peptide histidine isoleucine amide-27-like peptide (PLP) was investigated in rat brain applying different subcellular fractionation techniques. Unlike VIP or PLP, motilin-like peptides were not located in synaptosomes, but in the cell nucleus. This is the first report of a non-vesicular localization of this neuropeptide and is suggestive of a possible non-neurotransmitter role of MLIP. Previous developmental studies point to a possible role for motilin-like peptides as trophic or developmental factors. These results open the possibility that brain motilin-like peptides may operate by binding to chromatin and regulating gene expression.
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DM
Korchak
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M
Laskowski
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MC
Beinfeld
D000818:Animals; D001923:Brain Chemistry; D002458:Cell Fractionation / Q000379:methods; D002467:Cell Nucleus / Q000032:analysis* / Q000648:ultrastructure; D004247:DNA / Q000032:analysis; D008854:Microscopy, Electron; D009037:Motilin / Q000032:analysis*; D009419:Nerve Tissue Proteins / Q000032:analysis; D010455:Peptides / Q000032:analysis; D012313:RNA / Q000032:analysis; D051381:Rats; D013347:Subcellular Fractions / Q000032:analysis
D009419:Nerve Tissue Proteins; D010455:Peptides; D009037:Motilin; D012313:RNA; D004247:DNA