A phospho-oligosaccharide can reproduce the stimulatory effect of insulin on glycolytic flux in human fibroblasts.
It has been recently demonstrated that insulin promotes the hydrolysis of a glycosyl-phosphatidylinositol, stimulating the release of a phospho-oligosaccharide which displays several insulin-like effects. In the present study we have investigated whether the compound is able to mimic insulin action on glucose metabolism in human fibroblasts. Similarly to the hormone, the phospho-oligosaccharide elicited a dose dependent increase in lactate output and fructose 2,6-bisphosphate content. The effect of the compound was time dependent with a progressive increase starting from 2 hours of incubation. 1 microM phospho-oligosaccharide had half maximal effect on both parameters, increasing glycolytic flux by approximately 30% and fructose 2,6-bisphosphate content by 70%. Therefore the phospho-oligosaccharide appears to be able to strictly reproduce insulin action on glucose metabolism in human fibroblasts.
Dipartimento di Scienze Biochimiche, Università di Firenze, Italy.
P
P
Bruni
E
E
Meacci
M
M
Avila
V
V
Vasta
M
M
Farnararo
J M
JM
Mato
I
I
Varela
D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism*; D005636:Fructosephosphates / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects*; D017261:Glycosylphosphatidylinositols; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D007773:Lactates / Q000378:metabolism; D009844:Oligosaccharides / Q000494:pharmacology*; D010716:Phosphatidylinositols / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*
D005636:Fructosephosphates; D017261:Glycosylphosphatidylinositols; D007328:Insulin; D007773:Lactates; D009844:Oligosaccharides; D010716:Phosphatidylinositols; D011134:Polysaccharides