[Detection of trophoblastic beta 1-glycoprotein in tumor tissue and blood in ovarian neoplasms].
Using immunochemical analysis methods (the reaction of precipitation in agar, immunoenzymatic method, immunofluorescence), trophoblastic beta 1-glycoprotein (TBG) concentration in tumour tissue and in the blood serum of patients with ovarian cancer was studied. By rabbits immunization with glycoprotein fraction of ovarian adenocarcinoma, dissolved in 0.6 M sulfosalicylic acid, the authors obtained antibodies to TBG. Immunoenzymatic method showed, that TBG level is raised during ovarian cancer (more than 3 micrograms/l): in 18% of tumour extracts, in 12.5% of blood sera samples and in 41.6% of cases in ascites fluid. Utilizing indirect immunofluorescence method morphological structures of trophoblastic type were identified in paraffin sections of ovarian adenocarcinoma. The authors suppose, that such structures may be responsible for TBG biosynthesis in ovarian tumours.
Prokopenko
P G
PG
Tatarinov
Iu S
IuS
Borisenko
S A
SA
Reshetova
T I
TI
Fomina
M V
MV
D000230:Adenocarcinoma / Q000032:analysis* / Q000097:blood / Q000175:diagnosis; D000818:Animals; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D007120:Immunochemistry; D009363:Neoplasm Proteins / Q000032:analysis* / Q000097:blood; D010051:Ovarian Neoplasms / Q000032:analysis* / Q000097:blood / Q000175:diagnosis; D011257:Pregnancy Proteins / Q000032:analysis*; D011268:Pregnancy-Specific beta 1-Glycoproteins / Q000032:analysis*; D011817:Rabbits
D009363:Neoplasm Proteins; D011257:Pregnancy Proteins; D011268:Pregnancy-Specific beta 1-Glycoproteins
Opredelenie trofoblasticheskogo beta 1-glikoproteina v opukholevoĭ tkani i syvorotke krovi pri rake iaichnika.