Oligoclonal banding in cerebrospinal fluid assessed by electrophoresis on agarose after centrifugal sample concentration through a microconcentrator membrane.
We describe use of a microconcentrator membrane with a 30 000-Da cutoff for treatment of cerebrospinal fluid (CSF) specimens before detection of oligoclonal bands by electrophoresis on agarose. After centrifugation at 2000 X g for 25 min, 0.5-, 1.0-, and 2.0-mL aliquots of CSF were concentrated 15-, 25-, and 40-fold. Analytical recovery of immunoglobulins G and A from the microconcentrators was about 90% (CV 5-8%). We found good correlation between results by this method and by a silver-stain procedure in a study comparing oligoclonal banding in CSF from multiple sclerosis and control patients. After 40-fold concentration of 2 mL of CSF, 2 mg of immunoglobulin G per liter can be detected, because the analytical sensitivity of the electrophoresis is 80 mg/L.
R H
RH
Christenson
M E
ME
Russell
K T
KT
Gubar
L M
LM
Silverman
G C
GC
Ebers
D002498:Centrifugation; D004587:Electrophoresis, Agar Gel; D005374:Filtration; D006801:Humans; D007136:Immunoglobulins / Q000134:cerebrospinal fluid*; D009101:Multiple Myeloma / Q000097:blood / Q000134:cerebrospinal fluid; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid / Q000276:immunology; D043025:Oligoclonal Bands; D012834:Silver; D013194:Staining and Labeling
D007136:Immunoglobulins; D043025:Oligoclonal Bands; D012834:Silver