Hypothermic preservation of rabbit kidneys for 48 hours using low ionic strength solutions.
Microwaves offer the prospect of rapid and uniform heating of frozen organs. This is significant in the context of cryopreservation, and particularly of vitrification, because microwave heating may help to avoid crystallization or recrystallization of ice during warming, minimize any effects of high cell density, and reduce thermal-mechanical stresses. Previous work has established a rationale for reducing the ionic strength of solutions used to prepare tissues for microwave heating, since this permits the use of lower frequencies, which makes heating more uniform, without increasing the risk of thermal runaway (T. P. Marsland, S. Evans, and D. E. Pegg, Cryobiology 24, 311-323, 1987). In this paper we report a study of two possible low ionic strength perfusates, in rabbit kidneys, using 48 hr of hypothermic storage and autotransplantation as the test system. This model was chosen because there is a great deal of basic information about it. Both a single-pass "flush" preservation solution and a solution designed for continuous perfusion gave excellent results. The continuous perfusion system, which would be the more suitable for introducing cryoprotectants, gave five of five surviving animals with peak serum creatinine levels of 353-555 mumol/liter normal histology in three cases, and only very minor damage in the other two. There would therefore seem to be no obstacle to the use of perfusates having a low ionic strength in renal cryopreservations studies.
Laboratory of Nephropathology, Odense University, Denmark.
I A
IA
Jacobsen
D E
DE
Pegg
H
H
Starklint
P
P
Barfort
M P
MP
Diaper
D000818:Animals; D003404:Creatinine / Q000097:blood; D015925:Cryopreservation / Q000379:methods*; D005069:Evaluation Studies as Topic; D006358:Hot Temperature; D066298:In Vitro Techniques; D007668:Kidney / Q000502:physiology; D016030:Kidney Transplantation; D008872:Microwaves; D009926:Organ Preservation / Q000379:methods*; D009994:Osmolar Concentration; D010477:Perfusion; D011817:Rabbits; D012996:Solutions; D014182:Transplantation, Autologous
D012996:Solutions; D003404:Creatinine