Immunochemistry, physical chemistry and biology of 2',5'-oligoadenylates.
Monoclonal antibodies directed against 2-5A were developed and characterized. 2',5'-Oligoadenylate-protein complexes possess at least three distinct antigenic surfaces defined primarily by the ribose-phosphate backbone. A schematic model for the three epitopes is presented. Antibodies directed against 2-5A, in conjunction with other techniques, were employed to quantify 2-5A in various tissues of pathogen-free mice. Levels of 2-5A were in the range of 400-800 fmole/gm. Mice injected with poly(I).poly(c) or encephalomyocarditis virus (EMCV) showed elevated levels of 2-5A. Administration of poly(I).poly(C) or EMCV increased the level of 2-5A in different tissues to different extents. Raman spectroscopy was employed to explore the conformation of 2-5A. Distinct differences were observed in bands arising from the backbone portion of 2-5A relative to those of 3-5A. The most striking finding was the appearance of a strong, sharp band at 1460 cm-1 in spectra of 5'-monophosphorylated 2-5A's. This band was barely detectable in core and triphosphorylated 2-5A. Its presence suggests that 5'-monophosphorylated 2-5A's possess a unique conformational feature that distinguish them from cores and 5'-triphosphorylated forms.
M I
MI
Johnston
W G
WG
Hearl
J C
JC
White
J
J
Imai
P F
PF
Torrence
R W
RW
Williams
D000227:Adenine Nucleotides / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D004686:Encephalomyocarditis virus; D004769:Enterovirus Infections / Q000378:metabolism; D000939:Epitopes / Q000276:immunology; D007120:Immunochemistry; D051379:Mice; D009843:Oligoribonucleotides / Q000276:immunology* / Q000378:metabolism; D011070:Poly I-C / Q000494:pharmacology; D013059:Spectrum Analysis, Raman; D014018:Tissue Distribution
D000227:Adenine Nucleotides; D000911:Antibodies, Monoclonal; D000939:Epitopes; D009843:Oligoribonucleotides; C023505:2',5'-oligoadenylate; D011070:Poly I-C