2-Chloro-[3H]-adenosine binding in isolated rat kidney membranes.
Binding sites for 2-chloro-[3H]-adenosine were found to be present in rat kidney membranes. Binding was inhibited by adenosine receptor agonists (5'-N-ethylcarboxamide adenosine, NECA; L-phenylisopropyl adenosine, L-PIA) and antagonists (theophylline; 3-isobutyl-1-methylxanthine, IBMX) but unaffected by an adenosine uptake inhibitor (papaverine). The binding sites exhibited the properties of an adenosine A2 receptor: micromolar affinity for 2-chloroadenosine; nearly identical potencies of theophylline and IBMX for inhibition of binding; NECA more potent than L-PIA for inhibition of binding.
P H
PH
Wu
P C
PC
Churchill
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D015762:2-Chloroadenosine; D000241:Adenosine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D001667:Binding, Competitive; D066298:In Vitro Techniques; D007668:Kidney / Q000378:metabolism*; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011983:Receptors, Purinergic; D013806:Theophylline / Q000494:pharmacology
D011956:Receptors, Cell Surface; D011983:Receptors, Purinergic; D015762:2-Chloroadenosine; D013806:Theophylline; D000241:Adenosine; D015056:1-Methyl-3-isobutylxanthine