Antioxidant capacity of allopurinol in biological systems.
The antioxidant capacity of allopurinol was investigated in three biological systems by measurements of visible chemiluminescence, oxygen uptake and production of thiobarbituric acid reactive substances (TBARS). The addition of allopurinol to rat brain homogenates undergoing autoxidation and erythrocyte ghost membranes supplemented with 2,2'-azo-bis-(2-amidinopropane), in concentrations up to 2 mM, has a negligible effect on lipid peroxidation development. In erythrocyte ghost membranes exposed to gamma irradiation (9.5 Gy/min), allopurinol inhibits the radiation-induced lipid peroxidation with a Q(1/2) of 2.0 mM. It is suggested that allopurinol may have an alternative antioxidant pathway of action in biological systems, probably through a scavenging action upon hydroxyl radicals.
Fauré
M
M
Departamento de Química, Facultad de Ciencia, Universidad de Santiago de Chile.
Lissi
E A
EA
Videla
L A
LA
D000493:Allopurinol / Q000494:pharmacology*; D000578:Amidines / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D005720:Gamma Rays; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects*; D010101:Oxygen Consumption; D051381:Rats; D011919:Rats, Inbred Strains; D013858:Thiobarbiturates / Q000378:metabolism
D000578:Amidines; D000975:Antioxidants; D013858:Thiobarbiturates; D000493:Allopurinol; C046728:2,2'-azobis(2-amidinopropane)