Activation of rat glutathione transferases in class mu by active oxygen species.
The activities of rat glutathione transferases (GSTs) 3-3, 3-4, 4-4 in Class mu towards 1-chloro-2,4-dinitrobenzene (CDNB) but not 1,2-dichloro-4-nitrobenzene were increased up to 5-fold during preincubation with 0.4 mM xanthine and xanthine oxidase in 50 mM potassium phosphate, pH 7.8, containing 0.1 mM EDTA. The activated GST 3-4, purified by S-hexylglutathione affinity chromatography after the treatment, had a higher specific activity (130 units/mg) than that of the nontreated (35 units/mg), the Km and Vmax values for glutathione or CDNB also were increased. Other rat GSTs in Class alpha and pi were inactivated by the same treatment. In the presence of superoxide dismutase, the activation of GST 3-4 did not occur.
Second Department of Biochemistry, Hirosaki University School of Medicine, Japan.
T
T
Murata
I
I
Hatayama
K
K
Satoh
S
S
Tsuchida
K
K
Sato
D000818:Animals; D004137:Dinitrochlorobenzene / Q000494:pharmacology; D004789:Enzyme Activation; D005609:Free Radicals; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008024:Ligands; D008099:Liver / Q000201:enzymology*; D008297:Male; D010100:Oxygen; D051381:Rats; D011919:Rats, Inbred Strains; D013482:Superoxide Dismutase / Q000494:pharmacology; D019820:Xanthine; D014969:Xanthine Oxidase / Q000494:pharmacology; D014970:Xanthines / Q000494:pharmacology
D004137:Dinitrochlorobenzene; D005609:Free Radicals; D007527:Isoenzymes; D008024:Ligands; D014970:Xanthines; D019820:Xanthine; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase; D010100:Oxygen