[Kaposi's sarcoma: comparative immunohistochemical study and histogenic significance of endothelial markers].
The origin of spindle-shaped cells in Kaposi's sarcoma (KS) remains controversial. Non-specific histochemical reactions, electron microscopic examinations and immunostainings using antibody against factor VIII-related antigen (F VIII-RAG) and Ulex europaeus agglutinin I (UEAI) lectin as endothelial markers have given contradictory results. Immunohistochemical techniques were applied to 7 frozen skin biopsy specimen of KS from 5 elderly Mediterranean people and 1 renal allograft recipient, and a group of 27 other frozen cutaneous tumours including haemangio and lymphangiosarcomas, benign vascular lesions and various epithelial, melanocytic, fibrohistiocytic, fibrosarcomatous and muscular tumours. Using UEAI and antibodies against F VIII-RAG, HLA-DR and vimentin, a large proportion of positive KS spindle cells was found in all cases whereas cells were negative for keratin. Among the various immunoreactivity patterns observed in this study, a unique immunohistochemical profile was demonstrated for KS, angiosarcoma and endothelial cell, which strongly supports the endothelial origin of spindle cells in KS. Whereas F VIII-RAG, HLA-DR, vimentin and UEAI were sensitive endothelial markers, only F VIII-RAG appeared specific for endothelial cells since UEAI stained 2 squamous cell carcinomas and HLA-DR and vimentin were present in various mesenchymal and melanocytic tumours.
A
A
Bendelac
J
J
Kanitakis
B
B
Chouvet
J
J
Viac
J
J
Thivolet
D000328:Adult; D000368:Aged; D000941:Antigens / Q000032:analysis; D004727:Endothelium / Q000032:analysis*; D005169:Factor VIII / Q000032:analysis / Q000276:immunology; D006684:HLA-DR Antigens; D006394:Hemangiosarcoma / Q000032:analysis; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D007633:Keratins / Q000032:analysis; D037102:Lectins; D008875:Middle Aged; D037121:Plant Lectins; D012514:Sarcoma, Kaposi / Q000032:analysis* / Q000276:immunology / Q000473:pathology; D012878:Skin Neoplasms / Q000032:analysis; D014746:Vimentin / Q000032:analysis; D014841:von Willebrand Factor
D000941:Antigens; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D037102:Lectins; D037121:Plant Lectins; C036850:Ulex europaeus lectins; D014746:Vimentin; D014841:von Willebrand Factor; D007633:Keratins; D005169:Factor VIII
Sarcome de Kaposi: étude immunohistochimique comparative et intérêt histogénétique des marqueurs endothéliaux.