A phenyl-beta-galactoside-specific monoclonal antibody reactive with murine and rat NK cells.
The phenyl-beta-galactoside (phi-beta-gal)-specific monoclonal antibody (mAb) 49H.8 cross-reacts with the terminal disaccharide structure of the asialo GM1 (AGM1) molecule. It was found to react with phi-beta-gal determinants on murine and rat splenic natural killer (NK) cells, as measured by complement depletion studies. Flow cytometric analysis identified the antigen on two IL 2-dependent cloned murine NK cell lines and the rat large granular lymphocyte leukemia RNK. We have compared the 49H.8 reactivity to that of anti-AGM1 antisera (alpha-AGM1) on NK cells and a panel of NK related killer cells, including bone marrow-derived killer cells, lymphokine-activated killer cells (LAK), and anomalous killer cells (AK). We found that the 49H.8 specificity closely paralleled that of alpha-AGM1. When tested against Con A-reactive T cells, the 49H.8 mAb was less reactive than the alpha-AGM1, indicating that it may be a more specific marker for splenic NK populations than the alpha-AGM1.
Miller
V E
VE
Lagarde
A E
AE
Longenecker
B M
BM
Greenberg
A H
AH
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000502:physiology; D000937:Antigen-Antibody Reactions; D000961:Antilymphocyte Serum / Q000276:immunology; D002999:Clone Cells / Q000276:immunology; D003208:Concanavalin A / Q000494:pharmacology; D000939:Epitopes / Q000276:immunology; D005677:G(M1) Ganglioside; D005697:Galactosides / Q000276:immunology*; D006027:Glycosides / Q000276:immunology*; D006028:Glycosphingolipids / Q000276:immunology; D007376:Interleukin-2 / Q000502:physiology; D007694:Killer Cells, Natural / Q000276:immunology*; D007942:Leukemia, Experimental / Q000276:immunology; D008212:Lymphocyte Depletion; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D010529:Peritoneal Cavity; D051381:Rats; D011916:Rats, Inbred F344; D011919:Rats, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D000961:Antilymphocyte Serum; D000939:Epitopes; D005697:Galactosides; D006027:Glycosides; D006028:Glycosphingolipids; D007376:Interleukin-2; C007816:beta-galactoside; D003208:Concanavalin A; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside