Antibodies directed against guanylic acid and guanosine are nucleolus specific probes.
Purified antibodies against guanylic acid and guanosine binding to RNA at guanosine residues were used to probe human lymphocyte preparations by indirect immunofluorescence. Neither antibody gave any banding pattern with metaphase chromosomes but both showed binding to specific sites in the interphase nuclei. Evidence presented indicates that these sites are guanosine residues on rDNA transcripts at the nucleolar organizer regions.
Iswari
S
S
Krishnamurthy
D S
DS
Jacob
T M
TM
D000906:Antibodies / Q000276:immunology*; D002871:Chromosome Banding; D004275:DNA, Ribosomal / Q000032:analysis; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D006150:Guanine Nucleotides / Q000276:immunology*; D006151:Guanosine / Q000276:immunology*; D006157:Guanosine Monophosphate / Q000276:immunology*; D006801:Humans; D008214:Lymphocytes / Q000032:analysis; D008677:Metaphase; D012313:RNA / Q000378:metabolism; D013861:Thiocyanates; D014158:Transcription, Genetic
D000906:Antibodies; D004275:DNA, Ribosomal; D005452:Fluoresceins; D006150:Guanine Nucleotides; D013861:Thiocyanates; D006151:Guanosine; D012313:RNA; D006157:Guanosine Monophosphate; D016650:Fluorescein-5-isothiocyanate