A new method using a modified Mayer's hemalum at pH 6 for demonstrating mucous neck cells.
The mucous neck cells of gastric glands were stained with a modified Mayer's hemalum adjusted to pH 6 with saturated aqueous lithium carbonate. One gram of hematoxylin was dissolved in 1000 ml distilled water and 200 mg sodium iodate, 3 g potassium alum, 50 g chloral hydrate and 1 g citric acid were added to the solution. Prior to staining, the solution was adjusted to pH 6 with saturated aqueous lithium carbonate. Bromine oxidation and urea abolished the alum hematoxylin reactivity of the mucous neck cells.
M
M
Akita
E
E
Murata
K
K
Fujita
K
K
Kaneko
D000818:Animals; D001578:Benzopyrans; D005753:Gastric Mucosa / Q000166:cytology*; D006416:Hematoxylin; D006863:Hydrogen-Ion Concentration; D051381:Rats; D013194:Staining and Labeling / Q000379:methods*
D001578:Benzopyrans; D006416:Hematoxylin