Crosslinking of tRNA containing a long extra arm to elongation factor Tu by trans-diamminedichloroplatinum(II).
A tRNA containing a long extra arm, namely E. coli tRNA(Leu1) has been crosslinked to elongation factor Tu, with the crosslinking reagent trans-diamminedichloroplatinum(II). The nucleotide involved in the crosslinking was identified to be a guanosine in the variable region at position 47F or 47G.
Department of Biostructural Chemistry, University of Aarhus, Denmark.
N J
NJ
Rasmussen
F P
FP
Wikman
B F
BF
Clark
D001483:Base Sequence; D002855:Chromatography, Thin Layer; D002945:Cisplatin / Q000494:pharmacology*; D003432:Cross-Linking Reagents; D015180:Electrophoresis, Gel, Two-Dimensional; D006151:Guanosine / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010444:Peptide Elongation Factor Tu / Q000378:metabolism*; D012345:RNA, Transfer, Amino Acid-Specific / Q000378:metabolism*; D012356:RNA, Transfer, Leu / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism
D003432:Cross-Linking Reagents; D012345:RNA, Transfer, Amino Acid-Specific; D012356:RNA, Transfer, Leu; D006151:Guanosine; C034697:transplatin; D012260:Ribonucleases; D010444:Peptide Elongation Factor Tu; D002945:Cisplatin