Covalent linkage of phosphoinositides to myelin basic protein: in vivo occurrence and in vitro studies with experimental allergic encephalomyelitis.
We have previously reported the covalent attachment of phosphoinositides to myelin basic protein (MBP) in vitro. In this study, in vivo phosphoinositidation of MBP was observed to occur after intracerebral injection with [32p]HPO4(2-). In the in vitro study of experimental allergic encephalomyelitis, a four-fold increase in phosphoinositidation of MBP was observed in the myelin from the guinea pigs sacrificed during the development of motor dysfunction, as compared to the control group. A decrease (40%) was observed among most of the animals sacrificed before the onset of motor dysfunction.
K C
KC
Chiu
F
F
Westall
R A
RA
Smith
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000378:metabolism*; D005260:Female; D006168:Guinea Pigs; D007276:Injections, Intraventricular; D004676:Myelin Basic Protein / Q000378:metabolism*; D010710:Phosphates / Q000008:administration & dosage / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D011817:Rabbits
D004676:Myelin Basic Protein; D010710:Phosphates; D010716:Phosphatidylinositols; D000255:Adenosine Triphosphate