Influence of hypoosmotic and ammonium chloride-mediated haemolysis on the integrity of human mononuclear blood cells.
Side effects of both hypoosmotic and ammonium chloride-mediated hemolysis were compared looking for cellular integrity of human peripheral blood mononuclear cells. Recovery and viability of mononuclear cells significantly declined only following water treatment. Cell loss by lysis of non-erythrocyte cells (monitored by 51Cr release) preferentially occurred in the lymphocyte population resulting in a relative enrichment of monocytes (identified by peroxidase and esterase staining as well as phagocytosis of fluorescent latex particles). Consequences of this shifted monocyte/lymphocyte ratio for immunological tests are obvious. Necessity of red cell lysis and disadvantages referred especially to NH4Cl-induced white cell functional losses are outlined.
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D000293:Adolescent; D000328:Adult; D000643:Ammonium Chloride / Q000494:pharmacology; D002469:Cell Separation; D002470:Cell Survival; D004950:Esterases / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008214:Lymphocytes / Q000502:physiology*; D008875:Middle Aged; D009000:Monocytes / Q000201:enzymology / Q000502:physiology*; D009996:Osmotic Fragility; D010544:Peroxidases / Q000378:metabolism; D010587:Phagocytosis; D013194:Staining and Labeling
D000643:Ammonium Chloride; D010544:Peroxidases; D004950:Esterases