Somatic antigens of Pseudomonas aeruginosa. The structure of the O-specific polysaccharide chains of P. aeruginosa O11 (Lányi) lipopolysaccharides.
Lipopolysaccharides were isolated from the phenol layer on aqueous phenol extraction of cells of Pseudomonas aeruginosa O11 (Lányi classification), strains 170021 and 170040. On mild acid degradation of the lipopolysaccharides, with the subsequent gel-filtration on Sephadex G-50, neutral O-specific polysaccharides made up of 6-deoxysugars alone were obtained. Two 2-acetamido-2,6-dideoxy-L-galactose (LFucNAc), 2-acetamido-2,6-dideoxy-D-glucose (DQuiNAc) and L-rhamnose (LRha) residues were found to be the components of the strain 170021 polysaccharide repeating units; those of strain 170040 contained the same monosaccharides, but, instead of 2-acetamido-2,6-dideoxy-D-glucose residue, that of 2-acetamido-2,6-dideoxy-D-galactose (DFucNAc) was present. On the basis of the 13C nuclear magnetic resonance data, methylation analysis and three successive Smith degradations the following structures were determined for the polysaccharide repeating units: strain 170021----2) LRha(alpha 1----3)LFucNAc(alpha 1----3)LFucNAc(alpha 1----3)DQuiNAc(beta 1----; strain 170040,----2)LRha(alpha 1----3)LFucNAc-(alpha 1----3)LFucNAc(alpha 1----3)DFucNAc(beta 1----; differing from one another by configuration of C-4 of 2-acetamido-2,6-dideoxy-D-hexopyranose only.
Y A
YA
Knirel
I M
IM
Skvortsov
A S
AS
Shashkov
B A
BA
Dmitriev
N K
NK
Kochetkov
E S
ES
Stanislavsky
G M
GM
Mashilova
D000942:Antigens, Bacterial / Q000032:analysis*; D002845:Chromatography; D006027:Glycosides / Q000032:analysis; D007120:Immunochemistry; D008070:Lipopolysaccharides / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D009005:Monosaccharides / Q000032:analysis; D019081:O Antigens; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D012703:Serotyping
D000942:Antigens, Bacterial; D006027:Glycosides; D008070:Lipopolysaccharides; D009005:Monosaccharides; D019081:O Antigens