Detection of papillomaviruses in cutaneous fibromas of white-tailed and mule deer.
Naturally occurring cutaneous fibromas affecting white-tailed deer (Odocoileus virginianus) and mule deer (O hemionus), and cutaneous fibropapillomas of domestic cattle were tested for papillomavirus using indirect immunofluorescence (IF), peroxidase-antiperoxidase (PAP), and negative-stain electron microscopic techniques. Papillomavirus was consistently detected using rabbit antiserum against papillomavirus group-specific antigen in all mule deer fibromas and bovine fibropapillomas; only 16 of 28 white-tailed deer fibromas tested by IF and 9 of 15 tested by PAP were detected. Normal skin from white-tailed deer or cattle was consistently negative for virus. Similar results were obtained by negative-stain electron microscopic examination of partially purified tumor homogenates. Using deer fibroma virus or bovine papillomavirus type 1-specific antisera, viruses were typed by IF, PAP, and immunoelectron microscopy.
J P
JP
Sundberg
E S
ES
Williams
D
D
Hill
W D
WD
Lancaster
S W
SW
Nielsen
D000818:Animals; D003670:Deer; D000939:Epitopes; D005350:Fibroma / Q000382:microbiology / Q000662:veterinary*; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D027383:Papillomaviridae / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure; D012878:Skin Neoplasms / Q000382:microbiology / Q000662:veterinary*
D000939:Epitopes