Effect of anterior hypothalamic deafferentation and continuous growth hormone infusion on the hepatic synthesis of alpha 2u-globulin in the male rat.
Anterior hypothalamic deafferentation and infusion of human GH (hGH) in the normal male rat caused a marked reduction in the hepatic concentration of alpha 2u-globulin, an androgen-dependent protein. Although s.c. injections of hGH (twice-daily) resulted in more than a 50% reduction in the hepatic level of alpha 2u-globulin, the same dose of hGH when administered continuously through osmotic minipumps caused a threefold greater inhibition. The decreased hepatic concentration of alpha 2u-globulin after hGH administration was associated with corresponding changes in the hepatic level of translatable alpha 2u-globulin messenger RNA. Continuous infusion of hGH through osmotic minipumps and removal of the anterior hypothalamic influence on GH secretion by deafferentation also caused a marked reduction in the cytoplasmic androgen-binding activity of the rat liver. These results suggest that alterations in the level and pattern of GH secretion may influence hepatic androgen-binding activity and alpha 2u-globulin synthesis.
B
B
Chatterjee
W F
WF
Demyan
J A
JA
Gustafsson
M W
MW
Harris
T
T
Hökfelt
G
G
Norstedt
A K
AK
Roy
D000344:Afferent Pathways / Q000502:physiology*; D000510:Alpha-Globulins / Q000032:analysis / Q000096:biosynthesis*; D000818:Animals; D003593:Cytoplasm / Q000378:metabolism; D004343:Drug Implants; D013006:Growth Hormone / Q000494:pharmacology*; D007032:Hypothalamus, Anterior / Q000502:physiology*; D008099:Liver / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains
D000510:Alpha-Globulins; D004343:Drug Implants; D012333:RNA, Messenger; C013803:alpha 2u globulin; D013006:Growth Hormone