Structure of the gene encoding concanavalin A from Canavalia gladiata and its expression in Escherichia coli cells.
We have cloned and sequenced the gene encoding concanavalin A (Con A) from Canavalia gladiata. The sequence covers the whole transcribed region as well as the 5'- and 3'-untranscribed sequences. The coding sequence lacks introns. Con A expressed in Escherichia coli cells was purified by Sephadex G-50 affinity chromatography. The precursor of Con A expressed in E. coli undergoes a peptide cleavage and ligation in the same way as that synthesized during seed maturation.
Yamauchi
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D
Department of Biology, Tokyo Metropolitan University, Japan.
Minamikawa
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T
D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D003208:Concanavalin A / Q000235:genetics* / Q000302:isolation & purification*; D004247:DNA / Q000032:analysis; D004926:Escherichia coli / Q000378:metabolism*; D007887:Fabaceae / Q000235:genetics* / Q000378:metabolism; D017343:Genes, Plant; D005815:Genetic Code; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D037121:Plant Lectins; D010946:Plants, Medicinal; D014158:Transcription, Genetic
D037121:Plant Lectins; D003208:Concanavalin A; D004247:DNA