Epoxy-slide embedment of cytochemically stained tissues and cultured cells for light and electron microscopy.
A new method is described for embedding stained tissue sections, cells, cultured cells or organ cultures in a special polyethylene mold to form epoxy microscope slides (cast-a-slides). Cast-a-slides in which biological specimens are embedded may be examined by light microscopy and individual optimally stained cells or tissue areas selected for examination by various modes of electron microscopy or X-ray microanalysis. Cultured cells or organs can be grown, fixed, stained and embedded in epoxy in the same cast-a-slide mold. The cast-a-slides can be stored conveniently in the same manner as glass microscopy slides.
B L
BL
Giammara
J S
JS
Hanker
D000818:Animals; D002478:Cells, Cultured; D004852:Epoxy Compounds; D006652:Histological Techniques; D006801:Humans; D051379:Mice; D008853:Microscopy / Q000379:methods*; D008854:Microscopy, Electron / Q000379:methods*; D051381:Rats; D013194:Staining and Labeling
D004852:Epoxy Compounds