[Isolation of hematopoietic stem cells from peripheral blood of patients with hemoblastoses].
To isolate peripheral blood stem cells, a fraction of the mononuclear blood cell suspension, 51 leukapheresis procedures were conducted in 8 patients with hemoblastosis using the continuous-flow blood cell separator Fenwal CS-3000. The blood volume processed in a procedure was 10 l at a flow rate of 40-60 ml/min, the centrifuge speed was 1600 rpm. The standard computer program of the Separator was modified depending on the whole blood rate and the patient's hematocrit. The mean yield of mononuclear cells per run was 6.3 X 10(9). Leukapheresis has not significantly affected the patient's blood cell concentration. The mean content of granulocytes in the leucocyte suspension comprised 1.89%. No complications or side effects were observed in the patients during leukapheresis procedures.
Aleksanian
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MZh
Ryzhko
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Selezneva
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OM
D000328:Adult; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000009:adverse effects / Q000627:therapeutic use; D002469:Cell Separation / Q000379:methods; D003131:Combined Modality Therapy; D005260:Female; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006801:Humans; D007937:Leukapheresis; D015470:Leukemia, Myeloid, Acute / Q000097:blood / Q000628:therapy*; D015479:Leukemia, Myelomonocytic, Acute / Q000097:blood / Q000628:therapy*; D008214:Lymphocytes / Q000187:drug effects / Q000473:pathology*; D008231:Lymphopenia / Q000139:chemically induced / Q000517:prevention & control; D008297:Male; D008875:Middle Aged
Poluchenie stvolovykh gemopoéticheskikh kletok iz perifericheskoĭ krovi bol'nykh gemoblastozami.