Morphological and functional studies during aging at mitochondrial level. Action of drugs.
1. Mitochondria show morphological changes on aging: the volume density decreases and the total surface area or volume increase. 2. Biochemical studies indicated a decrease in the rate of oxygen consumption and ATP levels for increasing age. Measurements of calcium transport across the mitochondrial membrane revealed a decrease in accumulated calcium and an alteration in the uptake kinetics of the ion in older animals. 3. Theophylline and calcitonin action were also studied. In both age groups (young and old) theophylline shows an inhibitory effect on all the parameters studied (both morphological and biochemical). 4. Calcitonin showed no effect on morphological and respiratory parameters, although it increased calcium uptake into the mitochondrion to a lesser extent in the 24 month old animals.
de la Cruz
J
J
Departamento de Bioquïmica, Facultad de Medicina, Universidad Complutense, Madrid, España.
Burón
I
I
Roncero
I
I
D000255:Adenosine Triphosphate / Q000378:metabolism; D000375:Aging / Q000502:physiology*; D000818:Animals; D002116:Calcitonin / Q000494:pharmacology; D002118:Calcium / Q000493:pharmacokinetics; D005260:Female; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D010101:Oxygen Consumption; D051381:Rats; D011919:Rats, Inbred Strains; D013806:Theophylline / Q000494:pharmacology
D000255:Adenosine Triphosphate; D002116:Calcitonin; D013806:Theophylline; D002118:Calcium