X-ray film digestion: a semi-quantitative assay for proteolytic enzymes used in immunohaematology.
A simple semi-quantitative assay method for proteolytic enzyme solutions used in blood group serology is described. The method is an adaptation of a technique formerly used for assay of faecal trypsin and is based on the digestion of the gelatin coating of unexposed X-ray film. A Spearman rank correlation coefficient of 0.8 was obtained when the X-ray film technique was compared with a spectrophotometric method using azoalbumin as substrate. The method is not suitable as a reference technique or as a substitute for serological control, but is useful for monitoring or comparing enzyme preparations in some circumstances or situations.
Department of Pathology, Basingstoke District Hospital, UK.
J M
JM
Giles
D J
DJ
Rogers
D001788:Blood Grouping and Crossmatching; D004796:Clinical Enzyme Tests / Q000379:methods*; D003546:Cysteine Endopeptidases / Q000032:analysis*; D006801:Humans; D014962:X-Ray Film
D003546:Cysteine Endopeptidases