Recovery and measurement of specific RNA species from postmortem brain tissue: a general reduction in Alzheimer's disease detected by molecular hybridization.
The recovery of RNA from postmortem (PM) brain tissues was quantified by molecular hybridization. RNA degradation rates postmortem were faster in mice with herpes encephalitis than with uninfected mice, but clear ribosomal peaks could be seen up to 72 hr after death. In a comparison between frontal cortex samples from neurologically normal and Alzheimer's disease cases a reduction in ribosomal, poly A, preproenkephalin, and preprosomatostatin RNA levels was observed in the Alzheimer's disease group. This general reduction may be influenced by the cause of death as well as the pathology.
G R
GR
Taylor
G I
GI
Carter
T J
TJ
Crow
J A
JA
Johnson
A F
AF
Fairbairn
E K
EK
Perry
R H
RH
Perry
D000544:Alzheimer Disease / Q000378:metabolism*; D000818:Animals; D001923:Brain Chemistry; D002540:Cerebral Cortex / Q000032:analysis; D004247:DNA; D004660:Encephalitis / Q000378:metabolism; D006561:Herpes Simplex / Q000378:metabolism; D006801:Humans; D051379:Mice; D009693:Nucleic Acid Hybridization; D011180:Postmortem Changes; D012313:RNA / Q000032:analysis*; D012335:RNA, Ribosomal / Q000032:analysis; D013997:Time Factors
D012335:RNA, Ribosomal; D012313:RNA; D004247:DNA