Mutagenicity experiments on L-cysteine and D-penicillamine using V79 cells as indicators and for metabolic activation.
Previous studies have shown that cysteine and penicillamine induce gene mutations in Salmonella typhimurium, the effect being strongly potentiated in the presence of mammalian tissue preparations. It has now been demonstrated that homogenate of V79 Chinese hamster cells is an efficient activator of thiol amino acids as well. Nevertheless, L-cysteine and D-penicillamine did not induce gene mutations (acquisition of resistance towards 6-thioguanine) in V79 cells. This was true even in the presence of the most efficient activating system, kidney postmitochondrial fraction. The result suggests the existence of an effective protective system in mammalian cells against the natural amino acid L-cysteine and its therapeutically used derivative, D-penicillamine.
Department of Toxicology, University of Mainz, F.R.G.
H
H
Glatt
D
D
Utesch
M
M
Herbst
F
F
Oesch
D000603:Amino Acids, Sulfur / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D003545:Cysteine / Q000633:toxicity*; D004351:Drug Resistance / Q000235:genetics; D007668:Kidney / Q000201:enzymology; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D009152:Mutagenicity Tests; D009153:Mutagens; D010396:Penicillamine / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains; D012486:Salmonella typhimurium / Q000187:drug effects; D013866:Thioguanine / Q000494:pharmacology
D000603:Amino Acids, Sulfur; D009153:Mutagens; D013866:Thioguanine; D010396:Penicillamine; D003545:Cysteine