A new combined quantitative cytophotometric method for estimating sulphydryl groups or disulphide bonds, and total protein in liver sections; applied to rats chronically exposed to cadmium.
A detailed procedure is described for a new combined quantitative cytophotometric method for estimating sulphydryl groups or (and) disulphide bonds as well as total protein. When applied to livers of cadmium treated rats and appropriate controls a dose response effect was demonstrated for the quantity disulphide bonds per unit protein.
A F
AF
Morselt
J H
JH
Peereboom-Stegeman
E J
EJ
Jongstra-Spaapen
D000818:Animals; D002104:Cadmium / Q000008:administration & dosage*; D003592:Cytophotometry / Q000379:methods*; D004220:Disulfides / Q000032:analysis*; D004305:Dose-Response Relationship, Drug; D005260:Female; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008668:Metallothionein / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling / Q000379:methods; D013438:Sulfhydryl Compounds / Q000032:analysis*; D013997:Time Factors
D004220:Disulfides; D013438:Sulfhydryl Compounds; D002104:Cadmium; D008668:Metallothionein