Intracellular processing of the gp160 HIV-1 envelope precursor. Endoproteolytic cleavage occurs in a cis or medial compartment of the Golgi complex.
The intracellular processing of the gp160 HIV-1 envelope precursor was characterized in acutely infected CD4+ T cells. Our data show that gp160 undergoes endoproteolytic cleavage by a nonacid dependent protease(s) in the rough endoplasmic reticulum-Golgi complex, within cis or medial cisternae, and is not transported to the cell surface. Two-dimensional electrophoretic pulse-chase analysis indicates that it takes greater than 2 h for gp160 to be transported from the rough endoplasmic reticulum to the site of action of sialyltransferases in the trans Golgi. Evidence is presented that gp160 is subject to mannose trimming in the Golgi complex, which is inhibited by 1-deoxymannojirimycin (a specific Golgi alpha-mannosidase I inhibitor). Preliminary data also suggest that gp120 is post-translationally modified by sialylated O-linked oligosaccharides.
Department of Pathology, Stanford University School of Medicine, California 94305.
B S
BS
Stein
E G
EG
Engleman
D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D015686:Gene Products, env / Q000235:genetics* / Q000302:isolation & purification; D006026:Glycoside Hydrolases; D006056:Golgi Apparatus / Q000378:metabolism*; D019302:HIV Envelope Protein gp160; D015497:HIV-1 / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008970:Molecular Weight; D009439:Neuraminidase; D011498:Protein Precursors / Q000235:genetics* / Q000302:isolation & purification; D011499:Protein Processing, Post-Translational; D013601:T-Lymphocytes
D015686:Gene Products, env; D019302:HIV Envelope Protein gp160; D011498:Protein Precursors; D006026:Glycoside Hydrolases; D009439:Neuraminidase