Immunologic heterogeneity of dihydrofolate reductase from methotrexate sensitive and resistant L1210 leukemia cells.
Dihydrofolate reductase from L1210 leukemia cells which are sensitive and resistant to methotrexate has the same physical and kinetic properties and immunoreactivity with a guinea pig antiserum raised to the enzyme purified from the methotrexate resistant strain. However, a chicken antiserum to dihydrofolate reductase from methotrexate sensitive L1210 cells has greater affinity for the homologous enzyme than for the enzyme from the MTX resistant cells indicating that there is some antigenic difference in these molecules.
M P
MP
Iqbal
S P
SP
Rothenberg
C
C
Larosa
D000818:Animals; D003600:Cytosol / Q000201:enzymology; D004351:Drug Resistance; D000939:Epitopes / Q000032:analysis; D007700:Kinetics; D007939:Leukemia L1210 / Q000188:drug therapy / Q000201:enzymology*; D008727:Methotrexate / Q000627:therapeutic use*; D051379:Mice; D011863:Radioimmunoassay; D013762:Tetrahydrofolate Dehydrogenase / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism
D000939:Epitopes; D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate