Nuclease S1-sensitive sites in multigene families: human U2 small nuclear RNA genes.
We show here that human U2 small nuclear RNA genes contain a 'strong nuclease S1 cleavage site' (SNS1 site), a sequence that is very sensitive to digestion by nuclease S1. This site is located 0.50-0.65 kb downstream of the U2 RNA coding region. It comprises a 0.15-kb region in which (dC-dT)n:(dA-dG)n co-polymeric stretches represent greater than 90% of the sequence. Nuclease S1 is able to excise unit length repeats of the human U2 RNA genes both from cloned fragments and total human genomic DNA. The precise locations of the cleavage sites are dependent on the superhelicity of the substrate DNA. In negatively supercoiled substrates, cleavages are distributed over the entire 0.15-kb region, but in linearized substrates, they occur within a more limited region, mainly at the boundary of the SNS1 site closest to the human U2 RNA coding region. Nuclease S1 cleavage of negatively supercoiled substrates occurs at pHs as high as 7.0; in contrast, cleavage of linearized substrates requires a pH less than 5.0, indicating that supercoiling contributes to the sensitivity of this site. Mung bean nuclease gives results similar to that observed with nuclease S1.
Htun
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Lund
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Westin
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Pettersson
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Dahlberg
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D001483:Base Sequence; D003001:Cloning, Molecular; D004720:Endonucleases / Q000378:metabolism*; D005796:Genes; D006801:Humans; D010944:Plants / Q000201:enzymology; D010957:Plasmids; D012313:RNA / Q000235:genetics*; D012342:RNA, Small Nuclear; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013379:Substrate Specificity
D012342:RNA, Small Nuclear; D012313:RNA; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases