Ribonuclease activities in rat sympathetic ganglia: evidence for the presence of an endogenous inhibitor of alkaline ribonuclease.
Using 3H-labeled rat brain mature RNA as substrate, substantial ribonuclease activity was detected in homogenates of rat superior cervical ganglia with acidic (pH 5.5) and neutral (pH 7.0-7.5) optima. Very little activity could be measured at greater than pH 8. The acidic and neutral activities differed in the optimal conditions required for assay, and showed differential sensitivity to the sulfhydryl blocking agent, N-ethylmaleimide. Only the neutral activity was stimulated, optimally by 2 mM N-ethylmaleimide, and the magnitude of stimulation indicated that the contributing ribonucleases exist largely in a latent form in the ganglion. Ribonucleases in other tissues with neutral pH dependence, known usually as "alkaline" ribonucleases, are subject to an N-ethylmaleimide-sensitive endogenous inhibitor protein. The existence of a similar inhibitor in rat superior cervical ganglia was indicated by the latency of neutral ribonuclease activity and confirmed by observing the effect of a soluble fraction from the ganglia on the activity of pancreatic ribonuclease A.
D J
DJ
Bates
R T
RT
Good
L
L
Austin
D000818:Animals; D005033:Ethylmaleimide / Q000494:pharmacology; D005615:Freezing; D005728:Ganglia, Sympathetic / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000494:pharmacology; D012313:RNA / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012260:Ribonucleases / Q000037:antagonists & inhibitors* / Q000378:metabolism
D012313:RNA; D012260:Ribonucleases; D008274:Magnesium; D005033:Ethylmaleimide