A new technique for retarding fading of fluorescence: DPX-BME.
The antioxidant beta-mercaptoethanol (BME) used in conjunction with the permanent mountant DPX (DPX-BME) retarded fluorescent fading of mithramycin, acridine orange and Hoechst 33258 stained chicken erythrocytes, each to a varying degree. The initial fluorescence of all dyes examined was more intense with DPX-BME than with DPX alone. Specimens mounted in DPX-BME showed strong fluorescence and excellent morphology; if kept in the dark, they could be stored indefinitely without deterioration. Retarding fading of fluorescence with DPX-BME faciliated quantitation of DNA using fluorescence cytophotometry.
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Franklin
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Filion
D000165:Acridine Orange; D000818:Animals; D006690:Bisbenzimidazole; D002645:Chickens; D004247:DNA / Q000032:analysis; D004912:Erythrocytes; D005453:Fluorescence; D005456:Fluorescent Dyes; D008623:Mercaptoethanol; D008926:Plicamycin; D013194:Staining and Labeling / Q000379:methods*
D005456:Fluorescent Dyes; D008623:Mercaptoethanol; D004247:DNA; D000165:Acridine Orange; D006690:Bisbenzimidazole; D008926:Plicamycin