Antigenic competition in IgE antibody production. III. Suppressive effect on Th and B cells.
An adoptive cell transfer system was utilized to evaluate the site of action of the suppressive mechanism involved in antigenic competition in IgE antibody production. Carrier-primed (OA) and hapten-primed (DNP-KLH) spleen cells were transferred to syngeneic irradiated recipients that were challenged with a heterologous conjugate (DNP-OA). To study the effect of antigenic competition on T and B cells, donor mice of one or the other cell type received in addition the competitor antigen (Asc) at immunization. The adoptive secondary IgE anti-DNP antibody response was suppressed in both situations. This effect could not be attributed to transfer of Asc-primed cells. Irradiation of donor mice before immunization with the two antigens abrogated the suppressive effect. These results indicate that both Th and B cells primed to the test antigen were affected by antigenic competition.
Soares
M F
MF
de Macedo
M S
MS
Perini
A
A
Mota
I
I
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology; D000939:Epitopes; D005260:Female; D006241:Haptens / Q000494:pharmacology; D007108:Immune Tolerance; D007116:Immunization, Passive; D007073:Immunoglobulin E / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D013154:Spleen / Q000166:cytology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D014916:Whole-Body Irradiation
D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D006241:Haptens; D007073:Immunoglobulin E