Reactivity of mouse ascitic fluids containing monoclonal antibodies directed against adenovirus hexon.
Thirty-two mouse hybridoma ascites were produced, containing hexon specific monoclonal antibodies, using crystallized hexons of human adenovirus type 1 as immunizing and selecting antigen. The reactivity of the ascitic fluids was tested with hexon preparations of 11 different human adenovirus species (serological types) belonging to 4 different subgenera by ELISA and passive haemagglutination (HA) methods. By these methods the 32 hybridoma antibodies showed 4 and 12 distinct reactivity patterns (type of cross-reactions) respectively. In the positive cases significant differences could be detected in the titres of the hybridoma antibodies in both ELISA and HA. It can be assumed that adenovirus type 1 hexon-related epitopes are present on a number of heterologous hexons in characteristic (interspecies) combinations for the given heterologous hexon and that a gradient of relationship may exist among the antigenic structures of hexons of the different adenovirus species.
Adám
E
E
Erdei
J
J
Lengyel
A
A
Katona
A
A
Berencsi
G
G
Fachet
J
J
Nász
I
I
D000260:Adenoviruses, Human / Q000145:classification / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D001202:Ascitic Fluid / Q000276:immunology*; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D006386:Hemagglutination Tests; D006801:Humans; D051379:Mice; D012703:Serotyping; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D000939:Epitopes; C018315:hexon capsid protein, Adenovirus