[The development of a radioimmunological method for determining autoantibodies to thyroglobulin].
A radioimmunoassay to determine a titer of autoantibodies to thyroglobulin in human blood serum was developed. The scheme of analysis included a reaction between 125I-thyroglobulin and autoantibodies to thyroglobulin with subsequent separation of the produced immune complex and free polyethylene glycol-labeled thyroglobulin. A high specificity of the radioimmunoassay is ensured by the use of highly purified thyroglobulin for obtaining the labeled agent. A possibility of the use of the method for the diagnosis of autoimmune thyroid diseases was shown.
A I
AI
Shcherban'
T S
TS
Cherkesova
A F
AF
Bogdanovich
V I
VI
Vasilevskiĭ
E P
EP
Demidchik
T I
TI
Minaĭlo
D001323:Autoantibodies / Q000032:analysis*; D002850:Chromatography, Gel; D005069:Evaluation Studies as Topic; D006111:Graves Disease / Q000276:immunology; D006801:Humans; D007457:Iodine Radioisotopes; D011863:Radioimmunoassay / Q000379:methods; D013954:Thyroglobulin / Q000276:immunology* / Q000302:isolation & purification; D013961:Thyroid Gland / Q000276:immunology; D013967:Thyroiditis, Autoimmune / Q000175:diagnosis
D001323:Autoantibodies; D007457:Iodine Radioisotopes; D013954:Thyroglobulin
Razrabotka radioimmunologicheskogo metoda dlia opredeleniia autoantitel k tireoglobulinu.