The effects of drugs on leucocyte changes following the injection of antigen into the peritoneal cavities of actively sensitised rats.
The injection of antigen into the peritoneal cavities of actively sensitised rats produced an immediate reaction characterised by an increase in concentrations in the peritoneal fluids, collected 5 min later, of extravasated dye labelled plasma proteins, histamine and slow reacting substance of anaphylaxis (SRS-A). Changes were also produced in the numbers of leucocytes in the blood and peritoneal cavity. 5 min after antigen challenge there was a reduction in the number of cells that could be washed from the peritoneal cavity. 4 h after antigen there was an increase in numbers of neutrophils both in the blood and peritoneal washings and these fell to the levels in control rats at 24 h. 24 h after antigen, and continuing for 72 h, there was an increase in numbers of eosinophils and mononuclear cells in the peritoneal washings. The rats were injected intravenously with sephadex particles to produce a blood eosinophilia at the time of antigen challenge, this increased the numbers of eosinophils migrating into the peritoneal cavity but had no effect on antibody levels, the numbers of other leucocytes or on the immediate reaction. An inhibitor of lipoxygenase and cyclo-oxygenase metabolism of arachidonic acid, phenidone, at 100 mg/kg p.o., inhibited SRS-A release to control levels, in the immediate reaction, but had no effect on the leucocyte changes. The glucocorticosteroid, dexamethasone, at doses of 0.1 and 1 mg/kg p.o., produced little inhibition of SRS-A release but significantly inhibited neutrophil, eosinophil and mononuclear cell infiltration into the peritoneal cavity.(ABSTRACT TRUNCATED AT 250 WORDS)
B A
BA
Spicer
S M
SM
Laycock
H
H
Smith
D000628:Aminophylline / Q000494:pharmacology; D000818:Animals; D000941:Antigens / Q000276:immunology*; D004205:Cromolyn Sodium / Q000494:pharmacology; D003533:Cyproheptadine / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D003911:Dextrans / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects; D006968:Hypersensitivity, Delayed; D006969:Hypersensitivity, Immediate; D007202:Indicators and Reagents / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D007958:Leukocyte Count; D007962:Leukocytes / Q000187:drug effects / Q000276:immunology*; D008297:Male; D011720:Pyrazoles / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013189:SRS-A / Q000096:biosynthesis
D000941:Antigens; D003911:Dextrans; D007202:Indicators and Reagents; D011720:Pyrazoles; D013189:SRS-A; D000628:Aminophylline; D003533:Cyproheptadine; D003907:Dexamethasone; C025614:sephadex; C015429:phenidone; D007545:Isoproterenol; D004205:Cromolyn Sodium