Glutamate and kainate are not directly toxic to developing amacrine cells: analysis in a Lucifer Yellow-labeled population.
Lucifer Yellow (LY)-labeled retinal amacrine cells were examined for toxin sensitivity to glutamate (Glu) or kainate (KA) and for high-affinity uptake (HAU) of Glu. In vitro, a 24-h exposure to either toxin caused a 50% loss of neurons with no loss of LY neurons. A 1-h exposure of intact retina to the toxins resulted in a two-fold increase in non-viable cells with no change in LY neurons. While histological damage within the retina was seen following toxin exposure, the LY population appeared unaffected. HAU in vitro was found in 5% of neurons but never colocalized with LY neurons. These studies show that Glu and KA do not directly affect the LY-labeled amacrine cells and support the hypothesis that their eventual loss may be an indirect consequence of toxin exposure.
Zeevalk
G D
GD
Hyndman
A G
AG
D000818:Animals; D002452:Cell Count / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D004351:Drug Resistance; D005456:Fluorescent Dyes; D005971:Glutamates / Q000633:toxicity*; D018698:Glutamic Acid; D007546:Isoquinolines; D007608:Kainic Acid / Q000633:toxicity*; D009474:Neurons / Q000145:classification / Q000187:drug effects* / Q000502:physiology; D012160:Retina / Q000166:cytology*; D013194:Staining and Labeling
D005456:Fluorescent Dyes; D005971:Glutamates; D007546:Isoquinolines; D018698:Glutamic Acid; C017475:lucifer yellow; D007608:Kainic Acid