Detection and determination of factor C--a regulatory protein--in Streptomyces strains by antiserum and monoclonal antibody.
Rabbit antisera and monoclonal antibodies were raised against factor C, a regulatory protein of Streptomyces griseus. ELISA and immunoblotting techniques suitable to determine and characterize factor C antigen was detected in all the 23 Streptomyces strains and variants examined thus far and in one Bacillus subtilis too. Depending on the strain analysed it has a molecular mass of 34,000 or 70,000 in mycelial homogenates. Most of factor C was found excreted into the cultivation medium. The quantity of factor C antigen in different Streptomyces strains showed great variation. Amy+ strains were usually good producers of factor C while Amy- were not. This was consistent with our assumption that factor C was an inducer of reproductive phase in Streptomyces.
Institute of Biology, University Medical School, Debrecen, Hungary.
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Szeszák
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Vitális
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Tóth
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Valu
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Fachet
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Szabó
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000942:Antigens, Bacterial / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D007106:Immune Sera / Q000276:immunology*; D015151:Immunoblotting; D011817:Rabbits; D013302:Streptomyces / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D007106:Immune Sera