Production of fumonisin B1 by Fusarium moniliforme NRRL 13616 in submerged culture.
Fumonisin B1, a recently discovered mycotoxin, was synthesized by submerged cultures of Fusarium moniliforme NRRL 13616 grown for 29 days at 28 degrees C and 220 rpm in a basal salts medium (pH 5.0) supplemented with 90 g of glucose per liter and 3.5 g of ammonium sulfate per liter. Under these culture conditions, 74 +/- 23 micrograms of fumonisin B1 per ml was produced by 29-day-old F. moniliforme NRRL 13616 cultures. Fumonisin B1 was detected in liquid culture extracts by high-performance thin-layer chromatography. Fumonisin B1 was confirmed and quantitated by gas chromatography and gas chromatography-mass spectral analysis of the trimethylsilyl derivative. The use of a defined medium for producing fumonisin B1 in a submerged culture facilitates its isolation and provides an excellent method for conducting biosynthetic studies.
Fermentation Biochemistry Research, U.S. Department of Agriculture, Peoria, Illinois 61604.
M A
MA
Jackson
G A
GA
Bennett
D002274:Carcinogens, Environmental / Q000378:metabolism; D003470:Culture Media; D037341:Fumonisins; D005670:Fusarium / Q000254:growth & development / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D009183:Mycotoxins / Q000096:biosynthesis*
D002274:Carcinogens, Environmental; D003470:Culture Media; D037341:Fumonisins; D009183:Mycotoxins; C056933:fumonisin B1