Interferon-mediated regulation of myc and Ki-ras oncogene expression in long-term-treated murine viral transformed cells.
Long-term treatment of a murine retroviral-transformed cell line (Ki-Balb) with 50 units/ml of interferon (IFN) resulted in a morphological reversion. The effects of IFN on myc and Ki-ras oncogene expression were examined after 6 months of treatment. mRNA dot and Northern blots hybridization analysis reveal that the expression of c-myc at the RNA level decreases by about fourfold. This reduction in the c-myc mRNA appears to be selective since in the same cells v-Ki-ras and an endogenous retroviral gene, intracisternal A particles (IAP), are increased four- and threefold, respectively. No significant inhibition of cellular growth and cell-cycle distribution was observed in IFN-Ki-Balb-treated cells.
R
R
Emanoil-Ravier
F
F
Pochart
M
M
Canivet
M
M
Garcette
J
J
Tobaly-Tapiero
J
J
Peries
D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D005786:Gene Expression Regulation / Q000187:drug effects*; D007181:Inclusion Bodies, Viral; D007372:Interferons / Q000494:pharmacology*; D007708:Kirsten murine sarcoma virus; D051379:Mice; D009857:Oncogenes / Q000187:drug effects*; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D012333:RNA, Messenger; D007372:Interferons