Rapid detection of Vibrio cholerae O:1 by motility inhibition and immunofluorescence with monoclonal antibodies.
Monoclonal antibodies against the group and type specific antigens of Vibrio cholerae O:1 lipopolysaccharide were used for the rapid detection of Vibrio cholerae strains by motility inhibition and immunofluorescence. Motility inhibition of live Vibrio cholerae O:1 was obtained with group specific monoclonal antibodies. Monoclonal antibodies against the type specific antigens B (Ogawa) and C (Inaba) inhibited motility of strains of homologous serotypes only. Indirect immunofluorescence of heatfixed bacteria with monoclonal antibodies and fluorescein-isothiocyanate conjugated rabbit anti-mouse immunoglobulin was also shown to be suitable for the rapid detection of Vibrio cholerae O:1. Both tests were highly specific and no cross-reactions were observed with strains of non-O:1 vibrios, Escherichia coli or Salmonella spp. tested. However, a weak fluorescence of some Ogawa strains was observed when high concentrations of Inaba specific monoclonal antibodies were used.
B
B
Gustafsson
T
T
Holme
D000818:Animals; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000276:immunology*; D002771:Cholera; D003429:Cross Reactions; D004926:Escherichia coli / Q000276:immunology; D005243:Feces / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009068:Movement; D019081:O Antigens; D012475:Salmonella / Q000276:immunology; D012703:Serotyping; D013045:Species Specificity; D014734:Vibrio cholerae / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D019081:O Antigens