Effective DNA cleavage by bleomycin-vanadium(IV) complex plus hydrogen peroxide.
It has been firstly found that the bleomycin-vanadyl(IV) complex is effectively capable of cleaving DNA in the presence of hydrogen peroxide. The 1:1 bleomycin-VO(IV) complex has been characterized by ESR and electronic absorption spectra, and its ESR parameters (go = 1.982 and Ao = 93.5 G) are indicative of VO(N5) coordination type for the metal-binding environment. The mode of nucleotide sequence cleavage induced by the present bleomycin-VO(IV)-H2O2 complex system was appreciably different from the corresponding Fe(III) complex system. Of special interest is the fact that the bleomycin-vanadium complex system more preferentially attacked G-A(5'----3') sequences than the bleomycin-iron complex system.
J
J
Kuwahara
T
T
Suzuki
Y
Y
Sugiura
D001483:Base Sequence; D001665:Binding Sites; D001761:Bleomycin / Q000494:pharmacology* / Q000031:analogs & derivatives; D004247:DNA / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D066298:In Vitro Techniques; D009942:Organometallic Compounds; D014639:Vanadium / Q000494:pharmacology*
D009942:Organometallic Compounds; C045703:bleomycin-vanadium(IV) complex; D014639:Vanadium; D001761:Bleomycin; D004247:DNA; D006861:Hydrogen Peroxide