Expression of extracellular and intracellular bullous pemphigoid antigens at the dermal-epibolic junction in organ culture of human skin.
Recent papers have reported that there are at least two distinct binding sites of bullous pemphigoid (BP) antibodies; one is the extracellular (lamina lucida) BP antigen (E-BPA) and the other is the intracellular (basal cell cytomembrane-hemidesmosome-cytoskeleton) BP antigen (I-BPA). In attempting to characterize the properties of these two BP antigens (BPA), we have investigated the expression of E-BPA and I-BPA as well as other basement membrane zone (BMZ) components such as type IV collagen, laminin, and epidermolysis bullosa acquisita (EBA) antigen at the dermal-epibolic junction as a model system for BMZ neogenesis. I-BPA was found along the dermal-epidermal junction and throughout the whole length of the dermal-epibolic junction. However, E-BPA and other BMZ components were found along the dermal-epidermal junction and along only the most proximal portion of the dermal-epibolic junction. These results suggest 1) that emergence of E-BPA varies from that of I-BPA in such a model system of BMZ neogenesis, and 2) that the emerged E-BPA may attach to the newly formed basement membrane complex.
Department of Dermatology, Juntendo University, School of Medicine, Tokyo, Japan.
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Ikeda
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Yaguchi
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Ogawa
D000906:Antibodies / Q000276:immunology; D000941:Antigens / Q000276:immunology*; D001485:Basement Membrane / Q000378:metabolism; D001666:Binding Sites, Antibody; D005110:Extracellular Space / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007158:Immunologic Techniques; D007425:Intracellular Membranes / Q000276:immunology*; D008854:Microscopy, Electron; D009924:Organ Culture Techniques; D010391:Pemphigoid, Bullous / Q000276:immunology* / Q000378:metabolism; D012867:Skin / Q000276:immunology* / Q000378:metabolism / Q000648:ultrastructure; D012872:Skin Diseases, Vesiculobullous / Q000276:immunology*
D000906:Antibodies; D000941:Antigens