Biosynthesis of lipid-linked oligosaccharides in embryonic liver. Formation of mannose containing derivatives.
In the presence of exogenous dolichyl phosphate mannosyl transferase activity towards dolichyl phosphate was nearly 3-fold higher in microsomes from pig embryonic liver compared to that from adult liver. After incubation of microsomes from embryonic liver with UDP-N-acetylglucosamine and GDP-[14C]mannose lipid-linked tri- to undecasaccharides were discovered in CHCl3-CH3OH (2:1, v/v) and CHCl3-CH3OH-H2O (1:1:0.3, by vol) extracts. The main proportion of the radioactivity was incorporated into penta-, sexta and undecasaccharides. Amphomycin at concentration 500 micrograms/ml inhibited almost completely dolichyl phosphate mannose synthesis in embryonic liver microsomes without inhibition the formation of lipid-linked penta- and sextasaccharides. It was suggested that mannose transferred to lipid-linked tetra- to heptasaccharides comes from GDP-mannose but not from dolichyl phosphate mannose.
S V
SV
Kyosseva
V I
VI
Zhivkov
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002250:Carbon Radioisotopes; D006017:Glycolipids / Q000302:isolation & purification; D006155:Guanosine Diphosphate Mannose / Q000378:metabolism; D055666:Lipopeptides; D008099:Liver / Q000196:embryology; D008364:Mannosyltransferases / Q000378:metabolism; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D009842:Oligopeptides / Q000494:pharmacology; D011104:Polyisoprenyl Phosphate Oligosaccharides / Q000096:biosynthesis*; D011105:Polyisoprenyl Phosphate Sugars / Q000096:biosynthesis*; D013552:Swine
D000900:Anti-Bacterial Agents; D002250:Carbon Radioisotopes; D006017:Glycolipids; D055666:Lipopeptides; D009842:Oligopeptides; D011104:Polyisoprenyl Phosphate Oligosaccharides; D011105:Polyisoprenyl Phosphate Sugars; C024962:dolichyl diphosphate oligosaccharides; D006155:Guanosine Diphosphate Mannose; C004423:amphomycin; D008364:Mannosyltransferases; C035777:chitobiosyldiphosphodolichol beta-mannosyltransferase