Immunohistochemistry of beta-neoendorphin and dynorphin in the endocrine pancreas of rat and man.
Serial sections from araldite-embedded rat and man pancreata were investigated immunohistochemically for the presence of prodynorphin-related peptides and alpha-endorphin. Immunoreactivities were visualized by the avidin/biotin-peroxidase complex (ABC) technique. In the human pancreas, none of the endocrine cells could be immunostained for prodynorphin-, proopiomelanocortin-related peptides and enkephalins. In the rat pancreas, however, all glucagon cells exhibited immunoreactivities for both beta-neoendorphin and dynorphin A. In addition, these cells contain alpha-endorphin-like immunoreactivity but no immunoreactivities for corticotropin, melanotropin, 16 K-fragment, alpha-N-acetyl-alpha-endorphin and enkephalins. All specificity controls confirmed that the rat endocrine pancreas might be an other source of dynorphin and endorphin with a biosynthetic pathway different from that in the pituitary or in other locations. However, concerning synthesis or degradation of peptide precursor substances interspecies differences may exist.
Cetin
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D000324:Adrenocorticotropic Hormone / Q000032:analysis; D000818:Animals; D004399:Dynorphins / Q000032:analysis*; D004745:Enkephalins / Q000032:analysis*; D005629:Frozen Sections; D006801:Humans; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D007515:Islets of Langerhans / Q000032:analysis* / Q000166:cytology; D010446:Peptide Fragments / Q000032:analysis; D011498:Protein Precursors / Q000032:analysis*; D051381:Rats; D013045:Species Specificity; D013194:Staining and Labeling
D004745:Enkephalins; D007106:Immune Sera; D010446:Peptide Fragments; D011498:Protein Precursors; D004399:Dynorphins; D000324:Adrenocorticotropic Hormone; C033660:preproenkephalin