Na+,K+-activated adenosine triphosphatase of isolated Müller cells from the rabbit retina shows a K+ dependence similar to that of brain astrocytes.
Müller (glial) cells from the rabbit retina were isolated by means of papain and mechanical dissociation. Their Na+,K+-adenosine triphosphatase (ATPase) activity was measured using a radiochemical method, and its K+ dependence was determined. In contrast to that of photoreceptors (data from the literature), the Na+,K+-ATPase of Müller cells could be shown to increase its activity greatly when the [K+] was enhanced up to 10 mM. The functional implications of this behaviour for the K+ clearance in the retina are discussed.
Reichenbach
A
A
Dettmer
D
D
Reichelt
W
W
Eberhardt
W
W
D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D001921:Brain / Q000166:cytology* / Q000378:metabolism; D002469:Cell Separation; D007473:Ion Channels / Q000378:metabolism; D009457:Neuroglia / Q000201:enzymology*; D010786:Photoreceptor Cells / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology*; D011817:Rabbits; D012160:Retina / Q000166:cytology* / Q000201:enzymology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D007473:Ion Channels; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium