Induction of cytochrome P450IA1 in rat hepatoma cell by polycyclic hydrocarbons and a dioxin.
In this study, the effects of benzo[a]pyrene (BP), 3-methylcholanthrene (3MC) and tetrachlorodibenzofuran (TCDBF) on the expression of the cytochrome P450IA1 gene in the rat hepatoma cell line H4IIE were examined. The initial rate of increase in the steady-state concentration of the mRNA for this gene was similar with each of these inducers; however, the elevated level of this mRNA was more sustained after TCDBF treatment. Nuclear run-off assays suggested that the elevated level of the mRNA was caused principally by an effect upon transcription.
Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, NH 03756.
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D000818:Animals; D001564:Benzo(a)pyrene / Q000494:pharmacology*; D001572:Benzofurans / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D004147:Dioxins / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D007527:Isoenzymes / Q000096:biosynthesis*; D008114:Liver Neoplasms, Experimental / Q000201:enzymology*; D008748:Methylcholanthrene / Q000494:pharmacology*; D000072317:Polychlorinated Dibenzodioxins / Q000494:pharmacology*; D051381:Rats; D014407:Tumor Cells, Cultured / Q000187:drug effects
D001572:Benzofurans; D004147:Dioxins; D007527:Isoenzymes; D000072317:Polychlorinated Dibenzodioxins; D001564:Benzo(a)pyrene; D008748:Methylcholanthrene; D003577:Cytochrome P-450 Enzyme System; C014211:2,3,7,8-tetrachlorodibenzofuran