[In vitro use of a model for studying the effects of tumor promoters in food].
Two antioxidants, butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA), which are widely used as food additives, have been proved in vivo to act as tumor promoters in rodent species. In order to study their mechanisms of action, BHT, BHA and phenobarbital (PB) were tested in vitro on liver epithelial cells isolated from 2-acetylaminofluorene-initiated rats. Interactions of BHT 3 x 10(-6) to 3 x 10(-5) M, BHA 10(-5) to 10(-4) M and of PB 10(-4) to 10(-3) M with cell growth on plastic dishes or in agarose, and with the expression of different proteins (gamma-glutamyltranspeptidase, cytoskeletal proteins, fibronectin) were followed.
Laboratoire de nutrition et de sécurité alimentaire, INRA-CRJ, Jouy-en-Josas, France.
P
P
Martel
C
C
Chaumontet
V
V
François
G
G
Pascal
D000818:Animals; D002083:Butylated Hydroxyanisole / Q000633:toxicity*; D002084:Butylated Hydroxytoluene / Q000633:toxicity*; D002273:Carcinogens / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology; D008954:Models, Biological; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D002273:Carcinogens; D002084:Butylated Hydroxytoluene; D002083:Butylated Hydroxyanisole; D010634:Phenobarbital
Utilisation d'un modèle in vitro pour l'étude des effets de promoteurs de tumeurs de l'alimentation.