Selective killing of smooth muscle cells in culture by the ricin A-chain conjugated with monoclonal antibodies to a cell surface antigen via a dextran bridge.
Monoclonal antibodies to a surface antigen of the modulated smooth muscle cells originally isolated from the rat aorta media were conjugated with ricin A-chain via an oxidized dextran bridge. The interaction of cultured cells with the conjugates obtained and with control substances was monitored following incorporation of 14C-leucine radioactivity. It was found that 14C-leucine incorporation was suppressed by 80-90% at a conjugate concentration of 10(-6)-10(-7) M. Antigen-negative cells (line IAR; rat hepatocytes) were insensitive to the conjugate at any concentration used. Control use of purified ricin A-chain, native or oxidized dextran, specific and nonspecific IgG did not affect normal 14C-leucine incorporation. The data obtained may be useful for designing targeted drug transport systems and for selective screening of modulated smooth cells in vascular pathology models in vivo.
Printseva OYu
A I
AI
Faerman
A V
AV
Maksimenko
A G
AG
Tonevitsky
O B
OB
Ilynsky
V P
VP
Torchilin
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000276:immunology; D001011:Aorta / Q000166:cytology; D002470:Cell Survival / Q000187:drug effects*; D002850:Chromatography, Gel; D003911:Dextrans; D007930:Leucine / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D012276:Ricin / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D003911:Dextrans; D012276:Ricin; D007930:Leucine