Interactions of soya-bean agglutinin with purified glycoconjugates and soya-bean seed components.
A radioaffinity assay for lectin binding to receptors was developed and characterized by using the interactions between soya-bean agglutinin and four glycoconjugates, namely thyroglobulin, galactomannan, fetuin and asialofetuin. On application of the assay to soya-bean extracts a wide range of seed components were found to have the capacity to interact with soya-bean agglutinin. These included both trichloroacetic acid-soluble and trichloroacetic acid-insoluble glycoconjugates and two classes of particulate matter distinguished by their differential solubility in Triton X-100.
Bond
H M
HM
Chaplin
M F
MF
Bowles
D J
DJ
D001212:Asialoglycoproteins; D060748:Fetuins; D005690:Galactose / Q000031:analogs & derivatives; D037102:Lectins; D008351:Mannans / Q000378:metabolism*; D017830:Octoxynol; D010936:Plant Extracts / Q000378:metabolism; D037121:Plant Lectins; D011092:Polyethylene Glycols; D011869:Radioligand Assay; D012639:Seeds / Q000378:metabolism; D030262:Soybean Proteins; D013025:Glycine max; D013954:Thyroglobulin / Q000378:metabolism*; D000509:alpha-Fetoproteins / Q000378:metabolism*; D010835:Phytohemagglutinins
D001212:Asialoglycoproteins; D060748:Fetuins; D005690:Galactose; D037102:Lectins; D008351:Mannans; D017830:Octoxynol; D010936:Plant Extracts; D037121:Plant Lectins; D011092:Polyethylene Glycols; D030262:Soybean Proteins; D013954:Thyroglobulin; D000509:alpha-Fetoproteins; C017029:asialofetuin; C028824:soybean lectin; D010835:Phytohemagglutinins; C012990:galactomannan