Synchronization of human lymphocyte cultures by fluorodeoxyuridine.
A simple technique for synchronization of human lymphocyte cultures with fluorodeoxyuridine (FudR) is presented. The S-phase block induced by the FudR is released by simultaneous exposure to 5-bromodeoxyuridine (BrdU) and Hoechst 33258 or by thymidine and Hoechst 33258. This method provides a high mitotic index with high percentage of prometaphase chromosomes. This simple method is highly advantageous and easy to utilize in clinical cytogenetics.
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De Braekeleer
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Keushnig
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Lin
D006690:Bisbenzimidazole / Q000494:pharmacology; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D002478:Cells, Cultured; D002871:Chromosome Banding; D005467:Floxuridine / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects; D008938:Mitosis / Q000187:drug effects; D013936:Thymidine / Q000494:pharmacology
D005467:Floxuridine; D001973:Bromodeoxyuridine; D006690:Bisbenzimidazole; D013936:Thymidine