The Rauscher-MuLV-induced leukemia, RBL-5, bears two tumor-associated transplantation antigens expressed on distinct molecules.
Tumor cells frequently express on their surface a new antigenic determinant which renders them immunogenic in the host animal. When immunity to this antigen results in rejection of a syngeneic tumor transplant, it is referred to as a tumor-associated transplantation antigen (TATA). RBL-5 is a Rauscher murine leukemia virus (MuLV)-induced leukemia of C57B1/6 origin that is potently immunogenic and shares a TATA with other tumors induced by the closely related Friend and Moloney-MuLVs (FMR-TATA). We have recently isolated a 175 kilodalton (kd) glycoprotein (gp175) which has all the properties expected of the FMR-TATA (Rogers et al., 1984). When this molecule was separated from a purified total glycoprotein fraction by DEAE chromatography, the remaining glycoproteins still contained a highly immunogenic TATA. Control experiments involving radioimmunoassay and immunoprecipitation with rabbit anti-gp175 indicated that this immunogenicity was not due to residual gp175 or breakdown products of gp175. We therefore conclude that RBL-5 expresses at least two distinct TATAs: gp175 and another glycoprotein distinguished from gp175 by its elution from a diethylaminoethyl-cellulose (DE52) column. These results, from a completely in vivo system, support data with other tumors obtained by in vitro methods and indicate that tumor cells may express several immunogenic molecules.
Galetto
G
G
Law
L W
LW
Rogers
M J
MJ
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D002460:Cell Line; D002848:Chromatography, DEAE-Cellulose; D003429:Cross Reactions; D000939:Epitopes / Q000235:genetics; D005622:Friend murine leukemia virus; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification; D006649:Histocompatibility Antigens / Q000032:analysis; D007942:Leukemia, Experimental / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008979:Moloney murine leukemia virus; D011925:Rauscher Virus; D014181:Transplantation Immunology
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D000939:Epitopes; D006023:Glycoproteins; D006649:Histocompatibility Antigens; C029237:tumor-associated transplantation antigen