Interleukin 1 increases the production of endothelin-1 by cultured endothelial cells.
We examined the effect of human recombinant interleukin 1 (IL-1) on the production of endothelin-1 by cultured porcine endothelial cells. The induction of endothelin-1 mRNA began within 1 hr of exposure to IL-1, showed twin peaks at 4 and 24 hr, and declined thereafter. Enzyme-linked immunosorbent assay revealed that the amount of endothelin-1 peptide in conditioned media was also increased by IL-1 in a dose- and time-dependent manner. Our results suggested that IL-1, a macrophage-derived cytokine, may affect the contraction and proliferation of vascular smooth muscle cells by stimulating the production of endothelin by endothelial cells.
Yoshizumi
M
M
Third Department of Internal Medicine, University of Tokyo, Japan.
Kurihara
H
H
Morita
T
T
Yamashita
T
T
Oh-hashi
Y
Y
Sugiyama
T
T
Takaku
F
F
Yanagisawa
M
M
Masaki
T
T
Yazaki
Y
Y
D000818:Animals; D001013:Aorta, Thoracic; D015152:Blotting, Northern; D002478:Cells, Cultured; D016232:Endothelins; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology*; D007700:Kinetics; D010452:Peptide Biosynthesis; D010455:Peptides / Q000235:genetics; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors; D011726:Pyridinium Compounds / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000494:pharmacology; D013552:Swine; D014158:Transcription, Genetic / Q000187:drug effects
D016232:Endothelins; D007375:Interleukin-1; D010455:Peptides; D010972:Platelet Activating Factor; D011726:Pyridinium Compounds; D012333:RNA, Messenger; D011994:Recombinant Proteins; C053321:CV 6209; D007213:Indomethacin