The antigenicity of synthetic peptide fragments of lactate dehydrogenase C4.
Lactate dehydrogenase C4 (LDH-C4) is an antigenic protein found only in spermatozoa and the mature testis. Synthetic peptides containing the amino acid sequences of the C-subunit, designated MC5-15, MC97-110 and MC211-220, were each conjugated to the carrier proteins diphtheria toxoid or bovine serum albumin. Rabbits immunized with these peptide-carrier conjugates produced antibodies to the peptide that cross-reacted with native LDH-C4. These data support our map that identifies antigenic domains of LDH-C4. Such synthetic peptides will be useful in the design of a contraceptive vaccine.
Wheat
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Shelton
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JA
Gonzales-Prevatt
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Goldberg
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D000818:Animals; D000917:Antibody Formation; D003429:Cross Reactions; D000939:Epitopes; D005260:Female; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology*; D011817:Rabbits; D011863:Radioimmunoassay
D000939:Epitopes; D007527:Isoenzymes; D010446:Peptide Fragments; D007770:L-Lactate Dehydrogenase