A chemiluminogenic substrate for N-acetyl-beta-D-glucosaminidase, o-aminophthalylhydrazido-N-acetyl-beta-D-glucosaminide.
A new type of chemiluminogenic substrate for N-acetyl-beta-D-glucosaminidase, o-aminophthalylhydrazido-N-acetyl-beta-D-glucosaminide, was prepared by incorporating an enzyme-removable substituent, N-acetyl-D-glucosaminide group, into the hydrazide moiety of luminol. This substrate releases luminol upon enzymatic hydrolysis. The enzyme was detected chemiluminescently using this substrate.
Sasamoto
K
K
Dojindo Laboratories, Kumamoto, Japan.
Ohkura
Y
Y
D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000378:metabolism; D000118:Acetylglucosaminidase / Q000378:metabolism*; D005944:Glucosamine / Q000031:analogs & derivatives*; D006596:Hexosaminidases / Q000378:metabolism*; D008163:Luminescent Measurements; D008165:Luminol / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism*; D011724:Pyridazines / Q000378:metabolism*
D011724:Pyridazines; C065472:3-aminophthalylhydrazido-N-acetyl-beta-glucosaminide; D008165:Luminol; D006596:Hexosaminidases; D000118:Acetylglucosaminidase; D005944:Glucosamine; D000117:Acetylglucosamine