Effects of environmental conditions on xylose fermentation by recombinant Escherichia coli.
In batch fermentations, optimal conversion of xylose to ethanol by recombinant Escherichia coli was obtained under the following conditions: 30 to 37 degrees C, pH 6.4 to 6.8, 0.1 to 0.2 M potassium phosphate buffer, and xylose concentrations of 8% or less. A yield of 39.2 g of ethanol per liter (4.9% ethanol by volume) was observed with 80 g of xylose per liter, equivalent to 96% of the maximum theoretical yield. Maximal volumetric productivity was 0.7 g of ethanol per liter per h in batch fermentations and 30 g of ethanol per liter per h in concentrated cell suspensions (analogous to cell recycling).
Department of Microbiology and Cell Science, University of Florida, Gainesville 32611.
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D002122:Calcium Chloride / Q000494:pharmacology; D003470:Culture Media; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005296:Ferrous Compounds / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008278:Magnesium Sulfate / Q000494:pharmacology; D013696:Temperature; D014994:Xylose / Q000378:metabolism*
D003470:Culture Media; D005296:Ferrous Compounds; C020748:ferrous sulfate; D000431:Ethanol; D008278:Magnesium Sulfate; D014994:Xylose; D002122:Calcium Chloride