Independent induction of interleukin 6 and prostaglandin E2 by interleukin 1 in human articular chondrocytes.
Interleukin 6 is a cytokine with growth and differentiation activities on a number of cell types. Human articular chondrocytes produce interleukin 6 and this production appears to be constitutive but can be stimulated in a dose-dependent manner by interleukin 1. Other stimulators of interleukin 6 production in chondrocytes include tumour necrosis factor-alpha, polyriboinosinic: polyribocytidylic acid and bacterial lipopolysaccharide. Interleukin 6 production is not inhibited by prostaglandin E2 but may be partially dependent on prostaglandin E2 production. Using an antiserum to interleukin 6 we have demonstrated that the production of prostaglandin E2 under basal conditions and in response to interleukin 1 is probably not mediated by interleukin 6.
Department of Human Metabolism and Clinical Biochemistry, University of Sheffield Medical School, UK.
R A
RA
Bunning
R G
RG
Russell
J
J
Van Damme
D002358:Cartilage, Articular / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D015232:Dinoprostone / Q000096:biosynthesis*; D004926:Escherichia coli; D006801:Humans; D007106:Immune Sera; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D009500:Neutralization Tests; D011070:Poly I-C / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D007106:Immune Sera; D007375:Interleukin-1; D015850:Interleukin-6; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D003208:Concanavalin A; D015232:Dinoprostone; D011070:Poly I-C; D007213:Indomethacin