Bacterial conversion of glycerol to beta-hydroxypropionaldehyde.
beta-hydroxypropionaldehyde (3-HPA) can be oxidized to acrylic acid, an industrially important chemical used in the manufacture of synthetic plastics and other polymers. Of 19 genera and 55 strains tested, 3 Klebsiella and 2 Enterobacter strains produced 3-HPA. The most efficient strain was Klebsiella pneumoniae NRRL B-4011. Under optimum conditions (28 degrees C; 40 g of semicarbazide hydrochloride per liter, 70 g of glycerol per liter; and pH 6.0), 3.1 g of B-4011 cells per liter accumulated 22 g of 3-HPA per liter at a specific rate of 0.83 g/g per h; however, 14.5 g of cells per liter accumulated 46 g of 3-HPA per liter at a specific rate of 0.41 g/g per h.
Northern Regional Research Center, U.S. Department of Agriculture, Peoria, Illinois 61604.
J E
JE
Vancauwenberge
P J
PJ
Slininger
R J
RJ
Bothast
D000447:Aldehydes; D004754:Enterobacter / Q000378:metabolism*; D004755:Enterobacteriaceae / Q000378:metabolism*; D005985:Glyceraldehyde / Q000031:analogs & derivatives* / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D007709:Klebsiella / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000378:metabolism*; D011407:Propane; D013997:Time Factors
D000447:Aldehydes; C047158:3-hydroxypropionaldehyde; D005985:Glyceraldehyde; D005990:Glycerol; D011407:Propane