[2 methodological approaches to the problem of the tissue specificity of transcription].
The determination of genome transcription level in different tissues or structures of organism is carried out at present by the method of DNA--RNA-hybridization in solutions using labeled unique DNA sequences and significant RNA excess. Similar results may be probably obtained by a more simple method--numerously repeated recycles of RNA passage through the columns with denaturated DNA polymerized in agarose gel. Hybridization of single-stranded DNAs with each other is excluded and it is possible to separate RNA into fractions specific for different tissues and organs.
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Vitvitskiĭ
D001921:Brain / Q000378:metabolism; D004247:DNA / Q000235:genetics; D004622:Embryo, Mammalian; D005796:Genes; D005821:Genetic Techniques; D006801:Humans; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D009928:Organ Specificity; D012313:RNA / Q000235:genetics; D013154:Spleen / Q000378:metabolism; D014158:Transcription, Genetic
D012313:RNA; D004247:DNA
Dva metodicheskikh podkhoda k probleme tkanevoĭ spetsifichnosti transkriptsii.