Histamine release induced from mast cells by active components of compound 48/80.
Compound 48/80 and 14C-labeled compound 48/80 were synthesized, and fractionated by thin-layer chromatography into 14 components (A-N) with various histamine releasing activities and different Ca2+ requirements for their actions. The histamine release induced from rat mast cells in vitro by the most active component, fraction D (molecular weight = 2280, a tridecamer composed of 13 monomer units), was greatly enhanced by extracellular Ca2+, and was partially reduced by pretreatment of the cells with dinitrophenylated ascaris antiserum, an IgE. In contrast, the histamine release induced by fraction H (molecular weight = 1580, a nonamer composed of 9 monomer units), was higher in Ca2+ -free medium than in Ca2+-containing medium, and was partially reduced by pretreatment of mast cells with neurotensin or substance P, Ca2+-independent releasers. Apparently both fractions D and H are useful reagents for investigating the role of Ca2+ in histamine release and releaser binding in mast cells.
Y
Y
Koibuchi
A
A
Ichikawa
M
M
Nakagawa
K
K
Tomita
D000818:Animals; D000947:Antigens, Helminth / Q000008:administration & dosage; D001200:Ascaris / Q000276:immunology; D001345:Autoradiography; D002118:Calcium / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects*; D007114:Immunization; D007073:Immunoglobulin E / Q000276:immunology; D066298:In Vitro Techniques; D007536:Isomerism; D008297:Male; D008407:Mast Cells / Q000187:drug effects*; D008970:Molecular Weight; D009496:Neurotensin / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013373:Substance P / Q000494:pharmacology; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology*
D000947:Antigens, Helminth; D013373:Substance P; D007073:Immunoglobulin E; D009496:Neurotensin; D003189:p-Methoxy-N-methylphenethylamine; D002118:Calcium