A calcium-activated potassium current in motor nerve terminals of the mouse.
Local circuit currents involving presynaptic terminals were recorded by micro-electrodes inserted into the perineurium of nerves from the triangularis sterni muscle of the mouse. A transient outward current component was isolated by blocking the voltage-activated (delayed rectifier) K current by 3,4-diaminopyridine (3,4-DAP). The amplitude of this component depended on external K concentration and fell to zero at [K]o = 15 mM. Since it also depended on [Ca]o, it was identified as a Ca-activated K current (IK(Ca)). Tetraethylammonium (TEA) (2 mM), Ba (2 mM), Co (10 mM) and Mn (2.5 mM) blocked IK(Ca). IK(Ca) decayed to zero in approximately 12 ms and recovered from inactivation in about 100 ms. Ca current was enhanced in inverse proportion to the degree of IK(Ca) depression. The possible role of IK(Ca) in the process of neuromuscular facilitation is briefly discussed.
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Mallart
D015761:4-Aminopyridine / Q000031:analogs & derivatives*; D000200:Action Potentials / Q000187:drug effects; D000077770:Amifampridine; D000631:Aminopyridines / Q000494:pharmacology; D000818:Animals; D001464:Barium / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology*; D003035:Cobalt / Q000494:pharmacology; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects; D008345:Manganese / Q000494:pharmacology; D051379:Mice; D009045:Motor Endplate / Q000502:physiology; D009469:Neuromuscular Junction / Q000502:physiology*; D011188:Potassium / Q000502:physiology*; D009435:Synaptic Transmission; D019789:Tetraethylammonium; D013757:Tetraethylammonium Compounds / Q000494:pharmacology
D000631:Aminopyridines; D007473:Ion Channels; D013757:Tetraethylammonium Compounds; D001464:Barium; D003035:Cobalt; D008345:Manganese; D019789:Tetraethylammonium; D015761:4-Aminopyridine; D000077770:Amifampridine; D011188:Potassium; D002118:Calcium