The influence of pergonal on in vitro production of placental protein 5 (PP5) by ovarian tumour cells.
A radioimmunoassay for the measurement of placental protein 5 (PP5) has been developed using a second antibody-polyethyleneglycol method to separate free from bound ligand. PP5 immunoreactivity was detected in culture media from a cell line derived from a small cell carcinoma of the ovary (SCCWm2). The culture-derived PP5 shares immunological identity with pregnancy and ovarian follicular PP5. Affinity interactions between PP5 and matrices such as heparin or thrombin-Sepharose were similar and independent of the origin of PP5. PP5 was heterogeneous with two forms, a monomer (18k) and dimer (36k) being detected in all biological fluids examined. The dimer was predominant in ovarian follicles and the SCCWm2 cell line, while the monomer predominated in pregnancy serum. The basal rate of PP5 secretion by the SCCWm2 cell line was 0.61AU/24h/10(5) cells. Incubation of cells in the presence of 150mIU Pergonal stimulated PP5 production 20.6 fold and following removal of Pergonal the production rate was maintained at 18.0 times the basal rate. No choriconic gonadotrophin, pregnancy-specific beta 1-glycoprotein, pregnancy associated plasma protein-A or alpha fetoprotein was detected in the culture medium of the SCCWm2 cell line.
Sinosich
M J
MJ
Dodd
J
J
Hudson
C N
CN
Tyler
J R
JR
Seppala
M
M
Grudzinskas
J G
JG
Saunders
D M
DM
D018288:Carcinoma, Small Cell / Q000378:metabolism*; D002460:Cell Line; D006063:Chorionic Gonadotropin / Q000032:analysis; D002846:Chromatography, Affinity; D003470:Culture Media; D005260:Female; D006023:Glycoproteins; D006801:Humans; D007122:Immunoelectrophoresis; D008596:Menotropins / Q000378:metabolism*; D006080:Ovarian Follicle / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000378:metabolism*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000378:metabolism*; D011266:Pregnancy-Associated Plasma Protein-A / Q000032:analysis; D011268:Pregnancy-Specific beta 1-Glycoproteins / Q000032:analysis; D011863:Radioimmunoassay; D000509:alpha-Fetoproteins / Q000032:analysis
D006063:Chorionic Gonadotropin; D003470:Culture Media; D006023:Glycoproteins; D011257:Pregnancy Proteins; D011268:Pregnancy-Specific beta 1-Glycoproteins; D000509:alpha-Fetoproteins; C021240:tissue-factor-pathway inhibitor 2; D008596:Menotropins; D011266:Pregnancy-Associated Plasma Protein-A