Identification of recurrent idiotypes within the unrestricted anti-fluorescein immune response.
The BALB/cV immune response to fluorescein (F1) was analyzed for expression of serologically defined idiotypes (Id) present on two anti-F1 hybridoma proteins, 3-13 and 3-17. After a I degree, II degree, or III degree immunization with F1-KLH, Id 3-13 and 3-17 were expressed in 15 of 28 and 22 of 28 mice, respectively. Solid-phase Id inhibition assays, performed by using pooled or individual anti-F1 antibody, indicated Id 3-13 and 3-17 represented less than 1.0% of the I degree, II degree, or III degree serum anti-F1 repertoire. Hybridoma proteins 3-13 and 3-17 had similar but distinct active sites as indicated by similar patterns of Id inhibition, fine specificity of binding, and affinity for F1. Analyses of 11 anti-F1-AECM-Ficoll hybridoma proteins (predominantly mu, lambda) indicated 4 of 11 clones were weakly cross-reactive with 3-13 and 3-17. Anti-F1-KLH II degree antibody from mice of various Igh allotypes was also screened and indicated these Id were expressed in each strain but at lower levels than in BALB/cV mice and were not strictly allotype associated. Id 3-13 and 3-17 represent the first demonstration of repertoire-specific recurrent Id expressed during a T-dependent immune response lacking a dominant Id.
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D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis / Q000096:biosynthesis*; D000915:Antibody Affinity; D002999:Clone Cells / Q000378:metabolism; D000939:Epitopes / Q000276:immunology; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins / Q000008:administration & dosage / Q000276:immunology*; D006433:Hemocyanins / Q000276:immunology; D007117:Immunization, Secondary; D007126:Immunoglobulin Allotypes / Q000032:analysis; D007130:Immunoglobulin Idiotypes / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008814:Mice, Inbred NZB; D011817:Rabbits; D013045:Species Specificity; D013861:Thiocyanates / Q000008:administration & dosage / Q000276:immunology*; D000338:beta-Aminoethyl Isothiourea / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D005452:Fluoresceins; D007126:Immunoglobulin Allotypes; D007130:Immunoglobulin Idiotypes; D013861:Thiocyanates; D000338:beta-Aminoethyl Isothiourea; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin; D016650:Fluorescein-5-isothiocyanate