Anti-actin antibodies. Chemical modification allows the selective production of antibodies to the N-terminal region.
The specific properties of sera elicited by various native, unfolded or chemically modified actins were compared to provide a means of obtaining high titres of antibodies directed against the N-terminal (1-39) or the central regions of the actin sequence. The antigenic structure of the N-terminal region of actin was analyzed. It has at least 2 discrete epitopes, one of which appears to be species-specific and is composed of the hydrophilic N-terminal heptapeptide sequence.
Y
Y
Benyamin
C
C
Roustan
M
M
Boyer
D000199:Actins / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D000939:Epitopes; D005561:Formates; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000276:immunology; D011487:Protein Conformation; D011817:Rabbits; D011863:Radioimmunoassay; D012995:Solubility
D000199:Actins; D000939:Epitopes; D005561:Formates; D010446:Peptide Fragments; C008817:peroxyformic acid