Microcalorimetric studies of the heats of solution of bovine myelin basic protein.
Heats of solution for myelin basic protein have been determined using microcalorimetry. All aqueous systems studied yielded negative heats of solution; in contrast, trifluoroethanol produced a small positive heat of solution, while reaction with dimethyl sulfoxide was strikingly exothermic. The heat of interaction for native myelin basic protein with 8 M urea at pH 4.0, 29 degrees C, was found to be -79 +/- 16 kcal/mol. The significance of these results in terms of the protein's structural organization is discussed.
C S
CS
Randall
R
R
Zand
D000818:Animals; D002151:Calorimetry / Q000379:methods; D002152:Calorimetry, Differential Scanning; D002417:Cattle; D008433:Mathematics; D004676:Myelin Basic Protein; D011487:Protein Conformation; D011489:Protein Denaturation / Q000187:drug effects; D013816:Thermodynamics; D014508:Urea / Q000494:pharmacology
D004676:Myelin Basic Protein; D014508:Urea