[Comparative immunochemical analysis of various human leukocyte interferons].
Using radioimmunological methods based on the use of mono- and polyclonal antibodies raised against interferon alpha A, it was shown that polyclonal antibodies quantitatively reacted not only with this protein, but also with interferons alpha F and alpha N, whereas all the variants of monoclonal antibodies studied reacted only with interferons alpha A and alpha N. Monoclonal antibodies 5A6, 11E9, 19C10, 258 and 268 are directed against overlapping epitopes of the interferon alpha A molecule, which simultaneously binds not more than two molecules of antibodies with different specificity. The correlation between immunochemical and biological activities of interferon alpha A during temperature denaturation and proteolytic degradation and its ability to form oligomeric complexes were investigated.
Kostrov
S V
SV
Izotova
L S
LS
Eremashvili
M R
MR
Galaktionov
K I
KI
Iurin
V L
VL
D000911:Antibodies, Monoclonal / Q000276:immunology; D001667:Binding, Competitive; D000939:Epitopes / Q000032:analysis; D006358:Hot Temperature; D006801:Humans; D006868:Hydrolysis; D007370:Interferon Type I / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000276:immunology; D010447:Peptide Hydrolases; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007370:Interferon Type I; D010447:Peptide Hydrolases
Sravnitel'nyĭ immunokhimicheskiĭ analiz nekotorykh leĭkotsitarnykh interferonov cheloveka.