Human hepatoma (Hep G2) cultures contain salt-resistant triglyceridase ("liver lipase").
The culture fluid of Hep G2 human hepatoma cells contains triglyceridase activity resistant to high-salt concentrations. The lipase binds to Sepharose-heparin columns from which it can be eluted by 0.8 to 0.9 M NaCl. The nature of this lipase was studied using antibodies raised against "liver" lipases from human and rat origin. The anti-rat liver lipase inhibits both the postheparin human and rat plasma enzyme while the anti-human liver lipase has no effect on the rat enzyme. The lipase of the Hep G2 cultures showed affinity to the antibodies raised against rat as well as human "liver" lipase as shown by inhibition experiments. These results show that Hep G2 cells secrete "liver" lipase and that there seems to exist a structural homology between the lipases from rat and human origin.
Persoon
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NL
Sips
H J
HJ
Jansen
H
H
D000818:Animals; D000906:Antibodies / Q000276:immunology; D006528:Carcinoma, Hepatocellular / Q000201:enzymology*; D002460:Cell Line; D002846:Chromatography, Affinity; D000939:Epitopes / Q000276:immunology; D006801:Humans; D008049:Lipase / Q000276:immunology / Q000378:metabolism*; D008113:Liver Neoplasms / Q000201:enzymology*; D009994:Osmolar Concentration; D051381:Rats; D012965:Sodium Chloride / Q000494:pharmacology; D013045:Species Specificity
D000906:Antibodies; D000939:Epitopes; D012965:Sodium Chloride; D008049:Lipase