Observation of tyrosine-O-phosphate in Drosophila melanogaster larvae by 31P-NMR spectroscopy.
31P-NMR spectra of intact larvae and pupae of Drosophila melanogaster have been obtained at 109.3 MHz. A major resonance in these samples has been identified as tyrosine-O-phosphate. Its chemical shift reflects the hemolymph plasma pH. Upon disruption of the organisms (necessary for chemical analyses of tyrosine-O-phosphate), phosphatases rapidly hydrolyze this phosphate ester, generating inorganic phosphate and free tyrosine.
R R
RR
Moore
C T
CT
Burt
M F
MF
Roberts
D000818:Animals; D004331:Drosophila melanogaster / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007814:Larva / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010710:Phosphates / Q000032:analysis; D019000:Phosphotyrosine; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis
D010710:Phosphates; D019000:Phosphotyrosine; D014443:Tyrosine