Murine transfer factor. III. Specific interactions between transfer factor and antigen.
The interactions between dialyzable transfer factor and antigens have been studied. Incubation of transfer factor-containing dialysates from ferritin-sensitized mice or ferritin-coated plastic surfaces removed the antigen-sensitizing activity; incubations of the same preparations on cytochrome c-coated surfaces did not. Similar results were obtained when cytochrome c-transfer factor was studied. Incubation on cytochrome c-coated surfaces removed the activity, but incubation on ferritin-coated surfaces did not. Specific transfer factor activities could be recovered by elution with 8 M urea or acetonitrile. The finding of interactions between transfer factor and antigens provides evidence for a molecular basis of the specificity of the immunologic effects of transfer factor. This technique may also enable us to obtain amounts of specific material that are adequate for chemical analysis.
Kirkpatrick
C H
CH
Rozzo
S J
SJ
Mascali
J J
JJ
D000818:Animals; D000941:Antigens / Q000032:analysis / Q000276:immunology*; D002417:Cattle; D010856:Columbidae; D003574:Cytochrome c Group / Q000276:immunology / Q000378:metabolism; D000939:Epitopes; D005260:Female; D005293:Ferritins / Q000276:immunology / Q000378:metabolism; D006736:Horses; D006968:Hypersensitivity, Delayed / Q000378:metabolism; D007163:Immunosorbent Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D011485:Protein Binding; D013154:Spleen / Q000166:cytology; D014165:Transfer Factor / Q000032:analysis / Q000276:immunology* / Q000378:metabolism
D000941:Antigens; D003574:Cytochrome c Group; D000939:Epitopes; D014165:Transfer Factor; D005293:Ferritins