Subcellular distribution of 2',5'-oligoadenylate synthetase in differentiating Friend leukemia cells.
The occurrence of distinct (2'-5')(A)n-synthetase activities has recently been documented in cytoplasmic and nuclear extracts of several interferon (IFN)-treated cell lines. Since a role has been proposed for (2'-5')(A)n synthetase in the control of cell growth and differentiation, we examined the subcellular distribution of (2'-5')(A)n-synthetase activity both in IFN-treated undifferentiated Friend leukemia cells (FLCs) and during dimethyl-sulfoxide (DMSO)-induced erythroid differentiation of FLCs. Both the nuclear and cytoplasmic (2'-5')(A)n activities were modulated to the same extent by IFNs and DMSO. No evidence for a causal relationship between enzyme activation and FLC differentiation was found.
N
N
Mechti
E
E
Affabris
G
G
Romeo
B
B
Lebleu
D015088:2',5'-Oligoadenylate Synthetase / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D002467:Cell Nucleus / Q000201:enzymology; D003593:Cytoplasm / Q000201:enzymology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004920:Erythropoiesis; D005622:Friend murine leukemia virus; D007372:Interferons / Q000494:pharmacology*; D004915:Leukemia, Erythroblastic, Acute / Q000201:enzymology* / Q000473:pathology; D007942:Leukemia, Experimental / Q000201:enzymology* / Q000473:pathology; D011863:Radioimmunoassay; D013347:Subcellular Fractions / Q000201:enzymology
D007372:Interferons; D015088:2',5'-Oligoadenylate Synthetase; D004121:Dimethyl Sulfoxide