The localization of human keratin proteins at cytological and histological levels in carcinomatous and sarcomatous lesions.
Immunoperoxidase staining for human keratin proteins was performed cytologically on samples from 90 patients with malignant tumors, and histologically on samples from 164 patients with malignant tumors. At the cytological level, almost all tumor cells not only in squamous cell carcinoma but also in nonsquamous cell carcinoma were positive for keratin proteins, in contrast with the apparent abscence of keratin proteins in sarcoma. At the histological level, almost all neoplastic cells of squamous cell carcinoma were positive for keratin proteins, the same as at the cytological level. In contrast, among cases of nonsquamous cell carcinoma, the frequency of appearance of keratin proteins varied according to the organ; it tended to be low in tumors with relatively good prognosis, such as carcinomas in the digestive system or thyroid cancer, and to be high in tumor with poor prognosis, such as pulmonary cancer, gallbladder cancer and endometrial cancer. However, there was a marked difference between the frequency of appearance of keratin proteins at the cytological level and that at the histological level, particularly in the cases of gastric cancer.
Y
Y
Yagi
Y
Y
Imamura
T
T
Saku
T
T
Shimotsu
M
M
Hatae
S
S
Yagi
D000906:Antibodies; D001943:Breast Neoplasms / Q000032:analysis; D003110:Colonic Neoplasms / Q000032:analysis; D005260:Female; D005706:Gallbladder Neoplasms / Q000032:analysis; D006651:Histocytochemistry; D006801:Humans; D007633:Keratins / Q000032:analysis*; D008175:Lung Neoplasms / Q000032:analysis; D009369:Neoplasms / Q000032:analysis*; D012509:Sarcoma / Q000032:analysis; D013274:Stomach Neoplasms / Q000032:analysis; D013964:Thyroid Neoplasms / Q000032:analysis; D014594:Uterine Neoplasms / Q000032:analysis
D000906:Antibodies; D007633:Keratins