Identification of Igh-C-linked determinants on suppressor T cell hybrids and factors specific for L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT).
Hyperimmunization of BALB/c mice with concanavalin A-stimulated blasts from the Ig allotype-congenic strain, C.B20, results in the production of antibodies reactive with T cells in an allotype-restricted manner. Spleen cells from these hyperimmune BALB/c mice were used to generate a panel of hybridomas that secrete monoclonal antibodies, reactive, in an allotype-restricted manner, exclusively with T cells subpopulations, and in particular, reactive with suppressor T cell hybridomas and their secreted soluble factors. Two functional classes of antibodies were identified: those that react with single polypeptide-chain suppressor T cell factors (TsF1) and the suppressor T cell hybridomas that produce such factors, and those that react with two polypeptide-chain suppressor T cell factors (TsF2) and their corresponding suppressor T cell hybridomas. These two classes of antibody were used to isolate molecules from the membranes of the respective suppressor T cell hybrids that are functionally and structurally related to the secreted suppressor T cell factors, suggesting a receptor function for these molecules.
Sorensen
C M
CM
Hayashi
R J
RJ
Pierce
C W
CW
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002874:Chromosome Mapping; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000276:immunology; D008222:Lymphokines / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains / Q000276:immunology; D010455:Peptides / Q000276:immunology*; D011108:Polymers; D011948:Receptors, Antigen, T-Cell / Q000276:immunology / Q000302:isolation & purification*; D013491:Suppressor Factors, Immunologic; D050378:T-Lymphocytes, Regulatory / Q000032:analysis / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007143:Immunoglobulin Heavy Chains; D008222:Lymphokines; D010455:Peptides; D011108:Polymers; D011948:Receptors, Antigen, T-Cell; D013491:Suppressor Factors, Immunologic; C020749:GAT