[Surface properties of L cells studied by countercurrent distribution in a 2-phase polymer system before and after cyochalasin B and colcemid exposure].
A study was made of the effect of cytochalazin B and cocemid on the line L cells of monolayer and suspension culture studied by the method of counter-current distribution in two-phase polymer system dextran-500 - polyethylenglycol-6000. It has been shown that cytochalazin B caused a decrease in the partition coefficient of both the subline cells irrespective of the growth phase of cell population. Colcemid decreases the partition coefficient in the log-phase of cell culture but increases it in the stationary phase. Effect of colcemid is not associated with the accumulation of metaphase cells in the cell population.
Iu P
IuP
Petrov
E V
EV
Andreeva
D000818:Animals; D002478:Cells, Cultured; D003377:Countercurrent Distribution; D003571:Cytochalasin B / Q000494:pharmacology*; D003599:Cytoskeleton / Q000187:drug effects / Q000648:ultrastructure; D003703:Demecolcine / Q000494:pharmacology*; D003911:Dextrans / Q000494:pharmacology; D007739:L Cells / Q000187:drug effects* / Q000648:ultrastructure; D008677:Metaphase / Q000187:drug effects; D051379:Mice; D011092:Polyethylene Glycols / Q000494:pharmacology; D013499:Surface Properties
D003911:Dextrans; D003571:Cytochalasin B; D011092:Polyethylene Glycols; D003703:Demecolcine
Issledovanie poverkhnostnykh svoĭstv L-kletok metodom protivotochnogo raspredeleniia v dvukhfaznoĭ polimernoĭ sisteme do i posle vozdeĭstviia na nikh tsitokhalazina B i koltsemida.