Large bowel carcinoma-specific antigens detected by the lectin, Griffonia simplicifolia agglutinin-II.
A lectin reactivity specific to human bowel carcinoma is reported. Twenty-six cases of carcinoma of the large intestine were examined. Normal as well as transitional mucosa and carcinoma tissues were removed from surgical specimens, and paraffin sections were stained with a battery of histochemical methods to characterize glycoconjugates, including high iron diamine-Alcian blue pH 2.5, modified PAS reaction to detect various sialic acids, paradoxical concanavalin A (Con A) staining, and stainings with 10 species of lectins labeled with horseradish peroxidase (HRP). Among the techniques employed, only Griffonia simplicifolia agglutinin-II (GS-II, specific to glucosamine)-HRP staining revealed highly selective affinity to the carcinoma tissues; the apical surface of the carcinoma cells stained most intensely. GS-II reactivity of the cells persisted after prior periodate oxidation, but was significantly enhanced by neuraminidase digestion. Comparison with two other lectin stainings with the same sugar specificity, viz. paradoxical concanavalin A staining and wheat germ agglutinin (WGA)-HRP staining, showed that the GS-II reactive sites lacked class III Con A reactivity but were possibly included in WGA reactive sites. The GS-II-HRP staining should be helpful in the identification of carcinoma tissue and for analysis of carcinoma-associated antigens.
Nakayama
J
J
Katsuyama
T
T
Ono
K
K
Honda
T
T
Akamatsu
T
T
Hattori
H
H
D000951:Antigens, Neoplasm / Q000032:analysis*; D002277:Carcinoma / Q000276:immunology*; D003110:Colonic Neoplasms / Q000276:immunology*; D003208:Concanavalin A; D006801:Humans; D007413:Intestinal Mucosa / Q000032:analysis / Q000276:immunology; D037102:Lectins / Q000378:metabolism; D009077:Mucins / Q000032:analysis*; D009439:Neuraminidase / Q000378:metabolism; D010084:Oxidation-Reduction; D010504:Periodic Acid; D037121:Plant Lectins; D013194:Staining and Labeling; D014909:Wheat Germ Agglutinins
D000951:Antigens, Neoplasm; C045475:Griffonia simplicifolia lectins; D037102:Lectins; D009077:Mucins; D037121:Plant Lectins; D014909:Wheat Germ Agglutinins; D010504:Periodic Acid; D003208:Concanavalin A; D009439:Neuraminidase