Effects of phosphorylcholine-lipopolysaccharide conjugates on the induction of anti-idiotype-mediated B-cell tolerance.
Preincubation of BALB/c spleen cell cultures for 24 hr with phosphorylcholine (PC)-containing antigen together with antibody against the major idiotype (id) of anti-PC antibody renders them irreversibly unresponsive to subsequent stimulation with the antigen alone. In contrast, cultures preincubated for 24 hr with anti-id antibody, either alone or together with lipopolysaccharide (LPS), resulted in an anti-PC response comparable to that induced in control cultures incubated with mock anti-id antibody. After such a 24-hr preincubation with anti-id antibody and various PC-LPS conjugates possessing intact activity for polyclonal B-cell activation, the anti-PC response was inversely proportional to the epitope (PC) density on the LPS conjugates. In addition, similar preincubation of cultures with a non-mitogenic low dose of PC-LPS in the presence of anti-id antibody induced suppression of the anti-PC response as observed with a specific antigen. These results suggest that specific epitope delivers an additional tolerogenic signal during induction of B-cell suppression by anti-id antibody. This epitope effect cannot be replaced by, and is antagonistic to, the mitogenic signal of LPS in the course of B-cell inactivation.
P B
PB
Massey
B S
BS
Kim
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D002794:Choline / Q000031:analogs & derivatives*; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes / Q000276:immunology; D007108:Immune Tolerance; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007700:Kinetics; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010767:Phosphorylcholine / Q000276:immunology*; D013154:Spleen / Q000276:immunology
D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D008070:Lipopolysaccharides; D010767:Phosphorylcholine; D002794:Choline