Pausing, switching and speed fluctuation of the bacterial flagellar motor and their relation to motility and chemotaxis.
Wild-type Escherichia coli and Salmonella typhimurium cells, tethered to glass by their flagella, rotate with brief intermittent pauses, the prevalence of which is decreased by attractants and increased by repellents. By attaching latex beads to filaments of a S. typhimurium mutant having straight rather than helical flagella, it was established that the flagella on free cells also pause intermittently. Pausing is therefore an intrinsic feature of the motor and not an artifact associated with tethering. In tethered cells of wild-type strains and non-chemotactic mutants defective in transducers, chemotaxis proteins, or the flagellar switch, both the classical response to chemotactic stimuli (change in direction of rotation from counterclockwise to clockwise or vice versa), and the pausing response to such stimuli, were linked together. No separate signal for pausing was found. In comparing different strains under different stimulation conditions, it was found that cells that never reversed seldom if ever paused, while cells that reversed frequently paused frequently. It is suggested that pausing is the result of futile switching events. A modified description of tumbling and chemotaxis is provided in which pausing, as well as reversal, has a role. Suppression of reversals and pauses by attractant stimuli commonly resulted in an increase in the speed of counterclockwise rotation; this may be because of suppression of pauses or reversals that are too brief to be detected. The clockwise rotation rate of unstimulated cells, which commonly was faster than their counterclockwise rate, was not further increased by repellent stimuli. The rotation rate of any given cell under any given condition was found to fluctuate on all time-scales measured. The study also revealed that some of the common repellents of E. coli and S. typhimurium slow down or stop the motor; these effects are not mediated by the chemotaxis machinery or intracellular pH.
Department of Membrane Research, Weizmann Institute of Science, Rehovot, Israel.
M
M
Eisenbach
A
A
Wolf
M
M
Welch
S R
SR
Caplan
I R
IR
Lapidus
R M
RM
Macnab
H
H
Aloni
O
O
Asher
D002474:Cell-Free System; D002633:Chemotaxis; D004926:Escherichia coli / Q000502:physiology*; D005407:Flagella / Q000502:physiology*; D005798:Genes, Bacterial; D066298:In Vitro Techniques; D009154:Mutation; D012486:Salmonella typhimurium / Q000235:genetics*