Template-dependent RNA polymerase from black beetle virus-infected Drosophila melanogaster cells.
Infection of cultured cells of Drosophila melanogaster with black beetle virus (BBV) induces an RNA polymerase that is bound to cellular particulate material in a complex with a template RNA. We have solubilized the polymerase by treatment of the relevant particulates with detergents such as dodecyl-beta-D-maltoside. The polymerase activity was made dependent upon exogenous RNA by destruction of the endogenous template RNA with micrococcal nuclease. Addition of BBV RNA1 or RNA2 induced synthesis of full-length negative-strand RNA isolated as a double-stranded complex with the added RNA. Newly synthesized plus strands were also detected in the RNA2 complexes. Certain other viral RNAs also induced synthesis of their negative strands.
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Saunders
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Kaesberg
D000818:Animals; D002478:Cells, Cultured; D001517:Coleoptera; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004331:Drosophila melanogaster; D007304:Insect Viruses / Q000201:enzymology*; D008836:Micrococcal Nuclease / Q000378:metabolism; D012313:RNA / Q000032:analysis / Q000096:biosynthesis; D018075:RNA, Complementary; D012330:RNA, Double-Stranded / Q000096:biosynthesis; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D013698:Templates, Genetic
D018075:RNA, Complementary; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D012313:RNA; D012321:DNA-Directed RNA Polymerases; D008836:Micrococcal Nuclease