A micromethod for measuring the molar concentration of polyadenylated RNA in the presence of ribosomal RNA.
A method has been developed for measuring the molar concentration of RNA and the mole fraction of polyadenylated RNA. Using known mixtures of globin mRNA and rRNA composed of 20 to 85% rRNA, the molar concentration of globin mRNA, a polyadenylated species, was determined in 45 min, with the consumption of less than 100 ng of total RNA. The technique is particularly well suited for determining the molar concentration of poly(A)+ RNA after chromatographic enrichment in columns of oligo(dT)-cellulose or poly(U)-Sepharose. The method makes possible the adoption of a molar standard.
Krug
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Berger
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D000818:Animals; D002417:Cattle; D004722:Endoribonucleases; D005914:Globins; D007700:Kinetics; D008832:Microchemistry / Q000379:methods*; D010761:Phosphorus Radioisotopes; D011061:Poly A / Q000032:analysis*; D011062:Polynucleotide Adenylyltransferase; D012313:RNA / Q000032:analysis*; D012333:RNA, Messenger; D012335:RNA, Ribosomal / Q000032:analysis*; D011817:Rabbits; D016914:Ribonuclease H; D013056:Spectrophotometry, Ultraviolet
D010761:Phosphorus Radioisotopes; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D011061:Poly A; D012313:RNA; D005914:Globins; D011062:Polynucleotide Adenylyltransferase; D004722:Endoribonucleases; D016914:Ribonuclease H