Double-staining techniques allows electrophysiological identification of monoamine-containing neurons.
Electrophysiological recording provides important evidence for positive identification of many neurons in gastropods. We describe a technique which combines intracellular recording and injection of a persistent, non-fluorescent dye (Fast Green) with subsequent histofluorescence treatment using a modification of the wholemount glyoxylic acid procedure developed by Barber (1983) to establish the presence or absence of monoamine transmitters in positively identified single gastropod neurons.
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D000818:Animals; D001679:Biogenic Amines / Q000032:analysis*; D001923:Brain Chemistry; D004298:Dopamine / Q000032:analysis; D004594:Electrophysiology / Q000379:methods*; D006038:Glyoxylates; D006651:Histocytochemistry / Q000379:methods*; D008195:Lymnaea; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000032:analysis*; D012394:Rosaniline Dyes; D012701:Serotonin / Q000032:analysis; D013194:Staining and Labeling / Q000379:methods*
D001679:Biogenic Amines; D006038:Glyoxylates; D012394:Rosaniline Dyes; D012701:Serotonin; C035906:Fast Green; C031150:glyoxylic acid; D004298:Dopamine