Development and characterization of antisera specific for amino- and carboxy-terminal regions of pp60src.
Antisera to the Rous sarcoma virus transforming protein pp60src were produced in rabbits immunized with p60src purified from recombinant Escherichia coli. Immunization with isolated V8 proteolytic fragments of p60src resulted in antisera specific for the amino- and carboxy-terminal domains. The reactions of these antisera with pp60v-src and the normal cell protein p60c-src were characterized.
M D
MD
Resh
R L
RL
Erikson
D000595:Amino Acid Sequence; D000918:Antibody Specificity; D001358:Avian Sarcoma Viruses; D000939:Epitopes; D007163:Immunosorbent Techniques; D015688:Oncogene Protein pp60(v-src); D009857:Oncogenes; D010446:Peptide Fragments / Q000276:immunology; D010750:Phosphoproteins / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology*
D000939:Epitopes; D010446:Peptide Fragments; D010750:Phosphoproteins; D014764:Viral Proteins; D015688:Oncogene Protein pp60(v-src)