Transport of benzenoid amino acids by system T and four broad scope systems in preimplantation mouse conceptuses.
We have studied transport of L-tryptophan, L-tyrosine and L-phenylalanine as factors contributing to homeostasis of these amino acids in preimplantation mouse conceptuses. Benzenoid amino acids were transported by the Na(+)-independent systems L and b0,+ in 1-cell conceptuses, and by these systems plus the Na(+)-dependent systems B0,+ and B in blastocysts. In addition, a component of Na(+)-independent tryptophan, tyrosine and phenylalanine transport in 1-cell and 2-cell conceptuses and in blastocysts resisted inhibition by L-leucine. The latter component of transport not only preferred benzenoid amino acids and in particular tryptophan as substrates, but it also was inhibited strongly and competitively by alpha-N-methyl-L-tryptophan. The leucine-resistant component of tryptophan transport also was inhibited strongly by N-ethylmaleimide and D-tryptophan, and it appeared to be inhibited weakly by 3-amino-endo-bicyclo[3.2.1]octane-3-carboxylic acid (BCO) but not by other amino acids tested as inhibitors. By these criteria, the leucine-resistant component of transport of benzenoid amino acids resembled system T in human red blood cells and rat hepatocytes. It is not entirely clear why preimplantation blastocysts have five good systems for transport of tryptophan. It is possible, however, that tryptophan homeostasis is particularly important during preimplantation development since it has been shown elsewhere that tryptophan availability in blood increases within one day after rat eggs are fertilized.
Department of Biochemistry, Chicago College of Osteopathic Medicine, Downers Grove, IL 60515.
L J
LJ
Van Winkle
D F
DF
Mann
A L
AL
Campione
B H
BH
Farrington
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001555:Benzene Derivatives / Q000378:metabolism*; D001692:Biological Transport; D001755:Blastocyst / Q000187:drug effects / Q000378:metabolism*; D004351:Drug Resistance; D047108:Embryonic Development; D005033:Ethylmaleimide / Q000494:pharmacology; D005260:Female; D006706:Homeostasis; D006801:Humans; D051379:Mice; D008815:Mice, Inbred Strains; D011247:Pregnancy; D051381:Rats; D012964:Sodium / Q000378:metabolism
D000596:Amino Acids; D001555:Benzene Derivatives; D012964:Sodium; D005033:Ethylmaleimide