Photo-induced nicking of deoxyribonucleic acid by ruthenium(II)-bleomycin in the presence of air.
A new metallobleomycin, ruthenium(II)-bleomycin A2 was prepared. When irradiated at 366 nm the ruthenium complex (1-10 microM) was found to break a 121-base pair DNA restriction fragment at specific sites (usually the pyrimidine residues located at the 3'-side of guanine). Care was taken to rule out complications due to the presence of trace amounts of iron. Photo-induced damage by cobalt-bleomycin in the presence of light, and redox-mediated damage by bleomycin and iron produced DNA cleavage at sites similar to ruthenium-bleomycin. In contrast to cobalt-bleomycin, ruthenium-bleomycin required oxygen as well as light to cause significant damage to DNA.
Subramanian
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Meares
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D000388:Air; D001483:Base Sequence; D001761:Bleomycin / Q000494:pharmacology* / Q000031:analogs & derivatives; D004247:DNA / Q000378:metabolism* / Q000528:radiation effects; D008027:Light; D009942:Organometallic Compounds; D010777:Photochemistry; D012428:Ruthenium / Q000494:pharmacology*
D009942:Organometallic Compounds; C047121:ruthenium(II)-bleomycin; D001761:Bleomycin; D012428:Ruthenium; D004247:DNA