Forskolin stimulation of 3, 5, 3'-triiodothyronine release from perifused rat thyroids.
In a perifusion system in the presence of 3-isobutyl-1-methylxanthine, forskolin stimulated secretion of not only cAMP but also 3, 5, 3'-triiodothyronine (T3) from rat thyroid glands. The increases in both cAMP and T3 were dose-dependent at forskolin concentrations of 2.0 X 10(-7)M to 2.0 X 10(-5)M. After perifusion for 4 h, tissue concentrations of cAMP also increased as a result of forskolin treatment. Since forskolin is regarded as a specific activator of the cAMP generating system, this observed forskolin stimulation of T3 secretion from perifused rat thyroid glands indicates that cAMP is involved in regulating thyroid hormone secretion.
Mashita
K
K
Moriwaki
K
K
Shimizu
M
M
Tajima
K
K
Tarui
S
S
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D005576:Colforsin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D013961:Thyroid Gland / Q000187:drug effects* / Q000378:metabolism; D014284:Triiodothyronine / Q000378:metabolism*
D014284:Triiodothyronine; D005576:Colforsin; D000242:Cyclic AMP; D015056:1-Methyl-3-isobutylxanthine