Histochemical detection of DNA strand scissions in mammalian cells by in situ nick translation.
A method to visualize in situ of single strand scissions of DNA in fixed mammalian cells has been developed. Using the nuclear nick translation with biotin-labeled dUTP followed by binding to avidin-biotin-peroxydase complex, the nuclei of HeLa cells which had been treated with a DNA-damaging antibiotic bleomycin were specifically stained, implicating that the histochemical detection of single strand scissions (nicks) of DNA in fixed cells was completed without destroying the morphology, and without using autoradiography.
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D001710:Biotin / Q000031:analogs & derivatives / Q000378:metabolism; D001761:Bleomycin / Q000494:pharmacology; D004256:DNA Polymerase I / Q000378:metabolism; D004260:DNA Repair; D004277:DNA, Single-Stranded / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism; D003856:Deoxyuracil Nucleotides / Q000378:metabolism; D006367:HeLa Cells / Q000187:drug effects / Q000378:metabolism*; D006651:Histocytochemistry; D006801:Humans; D014176:Protein Biosynthesis
D004277:DNA, Single-Stranded; D003856:Deoxyuracil Nucleotides; D001761:Bleomycin; D001710:Biotin; C045931:biotin-11-dUTP; D004256:DNA Polymerase I; D003850:Deoxyribonuclease I