A visible DNA-protein stain: Feulgen-Pararosanilin(SO2) Light Green.
Feulgen-Pararosanilin(SO2) Light Green, a DNA-protein stain, is described that is suited both for visual analysis and quantitative cytochemical measurement. The stain has been applied on cervical cells and quantitative aspects have been studied on chicken erythrocyte and rat liver nuclei. In comparison to single staining, the Feulgen-Pararosanilin(SO2) DNA content of the nuclei remains unaltered after application of the combined staining. The Light Green protein content is reduced considerably, however, dependent on the degree of chromatin condensation in the nucleus.
Oud
P S
PS
Pahlplatz
M M
MM
Hermkens
H G
HG
Tas
J
J
James
J
J
Vooijs
G P
GP
D000818:Animals; D002584:Cervix Uteri / Q000166:cytology; D002645:Chickens; D004396:Coloring Agents; D004247:DNA / Q000032:analysis*; D004912:Erythrocytes / Q000032:analysis; D005260:Female; D008099:Liver / Q000166:cytology; D009698:Nucleoproteins / Q000032:analysis*; D051381:Rats; D012394:Rosaniline Dyes; D013194:Staining and Labeling / Q000379:methods*; D014052:Toluidines
D004396:Coloring Agents; C007722:Feulgen stain; D009698:Nucleoproteins; D012394:Rosaniline Dyes; D014052:Toluidines; C005409:pararosaniline; D004247:DNA