Human monoclonal IgM autoantibodies with restricted antigenic specificity for myelin express unrelated idiotypes.
Idiotype-specific polyclonal antisera were prepared against myelin-binding human IgM paraproteins with specificity for the myelin-associated glycoprotein (MAG). Eight anti-idiotypic sera (6 against one monoclonal IgM and 2 against another) were tested by gel precipitation, immunoradiometric and ELISA assays for binding to the myelin-binding IgM paraproteins from 12 patients with demyelinating peripheral neuropathy. Anti-idiotypic antibodies bound only to the MAG-specific IgM used for immunization; there was no evidence of idiotypic cross-reactivity between the different IgM paraproteins.
Kahn
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D001323:Autoantibodies / Q000276:immunology*; D003711:Demyelinating Diseases / Q000150:complications* / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000032:analysis*; D007075:Immunoglobulin M / Q000276:immunology*; D009186:Myelin Sheath / Q000276:immunology*; D010265:Paraproteinemias / Q000150:complications* / Q000276:immunology; D010523:Peripheral Nervous System Diseases / Q000150:complications* / Q000276:immunology
D001323:Autoantibodies; D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D007075:Immunoglobulin M