Hydroxyapatite high-performance liquid chromatography: column performance for proteins.
Hydroxyapatite columns for high-performance liquid chromatography that are reusable for a long time were developed; performance tests were carried out by using several types of protein. Using a high flow rate, a sharp chromatographic peak can be obtained for a homogeneous molecule. A very high level of chromatographic separation can be achieved by decreasing the slope of the gradient and increasing the total column length at the same time.
Kawasaki
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T
Takahashi
S
S
Ikeda
K
K
D000327:Adsorption; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003094:Collagen / Q000032:analysis; D003461:Crystallography; D003574:Cytochrome c Group / Q000032:analysis; D003851:Deoxyribonucleases / Q000032:analysis; D017886:Durapatite; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D006882:Hydroxyapatites; D007782:Lactoglobulins / Q000032:analysis; D009113:Muramidase / Q000032:analysis; D009211:Myoglobin / Q000032:analysis; D009218:Myosins / Q000032:analysis; D010047:Ovalbumin / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D013499:Surface Properties; D014362:Trypsinogen / Q000032:analysis; D005719:gamma-Globulins / Q000032:analysis
D003574:Cytochrome c Group; D006454:Hemoglobins; D006882:Hydroxyapatites; D007782:Lactoglobulins; D009211:Myoglobin; D011506:Proteins; D005719:gamma-Globulins; D014362:Trypsinogen; D010047:Ovalbumin; D003094:Collagen; D017886:Durapatite; D003851:Deoxyribonucleases; D009113:Muramidase; D009218:Myosins