Relationship between the acceptor/donor radioactivity ratio and cross-linking in bacterial peptidoglycan: application to surface synthesis during the division cycle.
The relationship between the experimental measurement of the cross-linking of bacterial peptidoglycan and the mode of its insertion is analyzed. The cross-linking value, in practice and in theory, is independent of the pattern of strand insertion. Since the measure of the mode or pattern of insertion is the acceptor/donor radioactivity ratio (ADRR), no correction need be made for the ADRR. The measurement of cross-linking using radioactivity is independent of the labeling time, the specific activity of the label, and the mode of strand insertion. It is not concluded, however, that cross-linking does increase during the division cycle.
Max-Planck Institut fur Entwicklungsbiologie, Tubingen, Federal Republic of Germany.
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Cooper
D001419:Bacteria / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D002455:Cell Division; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D046911:Macromolecular Substances; D008954:Models, Biological; D010457:Peptidoglycan / Q000096:biosynthesis*
D046911:Macromolecular Substances; D010457:Peptidoglycan