Cloning and DNA sequence analysis of a polygalacturonase cDNA from Aspergillus niger RH5344.
A 1319 bp long cDNA encoding for a polygalacturonase (EC 3.2.1.15) from Aspergillus niger RH5344 comprises a single open reading frame of 1089 bp which includes the mature protein of 362 amino acids and an NH2-terminal signal peptide of 27 amino acids. The directly determined peptides of the mature polygalacturonase confirmed the sequence information deduced from the cDNA.
Ruttkowski
E
E
Institut für Biochemie, TH Darmstadt, F.R.G.
Labitzke
R
R
Khanh
N Q
NQ
Löffler
F
F
Gottschalk
M
M
Jany
K D
KD
D000595:Amino Acid Sequence; D001234:Aspergillus niger / Q000201:enzymology / Q000235:genetics*; D001322:Autoanalysis; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D004271:DNA, Fungal / Q000032:analysis*; D006026:Glycoside Hydrolases / Q000235:genetics*; D008969:Molecular Sequence Data; D011096:Polygalacturonase / Q000096:biosynthesis / Q000235:genetics*
D003062:Codon; D004271:DNA, Fungal; D006026:Glycoside Hydrolases; D011096:Polygalacturonase