Recent advances in laser microprobe mass analysis (LAMMA) of inner ear tissue.
Maintenance of ionic gradients within the various fluids compartments of the inner ear requires transport active cellular systems at different locations. LAMMA analysis is ideally suited for detection of ions in microquantity on cellular levels overcoming many technical difficulties. The present paper summarizes the results of microprobe analysis obtained with laser induced mass spectrometry (LAMMA) supplemented by X-ray microprobe analysis of epithelial cell layers adjacent to the endolymphatic space in the cochlear duct, in the vestibular organ and in the endolymphatic sac. The possible role of inner ear as well as ocular melanin in the mechanisms of active ion transport is discussed.
Meyer zum Gottesberge-Orsulakova
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Kaufmann
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D000818:Animals; D001693:Biological Transport, Active; D003053:Cochlear Duct / Q000032:analysis; D007758:Ear, Inner / Q000032:analysis*; D004577:Electron Probe Microanalysis; D004712:Endolymphatic Sac / Q000032:analysis; D006168:Guinea Pigs; D007473:Ion Channels / Q000032:analysis; D007761:Labyrinthine Fluids / Q000032:analysis*; D007834:Lasers; D013058:Mass Spectrometry / Q000379:methods*; D010860:Pigments, Biological / Q000032:analysis; D014722:Vestibule, Labyrinth / Q000032:analysis; D014882:Water-Electrolyte Balance
D007473:Ion Channels; D010860:Pigments, Biological