Sodium pathway markers in normal and kindled frog brains.
The present report evaluates Na,K-ATPase activity as well as Na channel levels in the frog telencephalon after kindling, i.e. the acquisition of an epileptic focus through localized low-voltage electrical stimulation of one hemisphere. K-dependent phosphatase activity and binding of tritiated ouabain were measured, revealing no change in Na,K-ATPase activity 14 h after the last seizure. Na channels were measured by binding assays using a tritiated ethylenediamine tetrodotoxin derivative. Na channels were reduced in kindled brain as compared to controls.
M E
ME
Moneta
M
M
de la Fuente
J L
JL
Liberona
E
E
Jaimovich
D009597:4-Nitrophenylphosphatase / Q000378:metabolism; D000818:Animals; D001001:Anura; D002352:Carrier Proteins / Q000032:analysis; D004558:Electric Stimulation; D007839:Functional Laterality / Q000502:physiology; D007473:Ion Channels / Q000201:enzymology / Q000378:metabolism* / Q000502:physiology; D007696:Kindling, Neurologic; D010042:Ouabain / Q000378:metabolism; D012964:Sodium / Q000502:physiology*; D015222:Sodium Channels; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013687:Telencephalon / Q000201:enzymology / Q000378:metabolism* / Q000502:physiology; D013779:Tetrodotoxin / Q000378:metabolism
D002352:Carrier Proteins; D007473:Ion Channels; D015222:Sodium Channels; C023790:tetrodotoxin-binding protein; D013779:Tetrodotoxin; D010042:Ouabain; D012964:Sodium; D009597:4-Nitrophenylphosphatase; D000254:Sodium-Potassium-Exchanging ATPase