Preparative scale separation of functionally distinct murine B cell subpopulations.
Mouse splenic B cells were found to be rapidly and reproducibly fractionated by adherence at 4 degrees C to plastic dishes pretreated with an alkaline buffer. The cell subsets obtained exhibited marked differences in size, surface immunoglobulins (sIg), and responses to B mitogens. Adherent B lymphocytes, bearing high densities of sIg, responded particularly to LPS whereas non-adherent B lymphocytes, with low densities of sIg, responded especially to dextran sulfate. The correlations between the separated populations and subsets at distinct maturation stages are discussed.
Chaby
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Girard
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D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000145:classification* / Q000276:immunology; D002448:Cell Adhesion; D002469:Cell Separation / Q000379:methods*; D016264:Dextran Sulfate; D003911:Dextrans / Q000494:pharmacology; D005434:Flow Cytometry; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D008264:Macrophages; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008808:Mice, Inbred CBA; D013154:Spleen / Q000166:cytology; D013234:Stem Cells
D000954:Antigens, Surface; D003911:Dextrans; D008070:Lipopolysaccharides; D016264:Dextran Sulfate