A self-Ia reactive T cell clone directly stimulates every hundredth B cell and helps antigen-specific B cell responses.
A self-I-A reactive T cell clone has been isolated that proliferates in the presence of irradiated syngeneic spleen cells in mouse serum. The cells directly stimulate approximately every hundredth B cell, which includes small B cells, into both proliferation and antibody formation in the absence of added antigen. There is evidence to suggest that cells of this type are generally present in murine T cell populations. The cells may recognize self-I-A as such. We failed to obtain evidence, but cannot exclude that they recognize an antibody idiotype in addition. The clone also augments antigen-specific antibody responses in vivo and in vitro in the presence of antigen. In the in vitro response to a T-independent TI-2 antigen, the T cells in co-culture with B cells induced specifically the production of IgG1 antibodies, thus mimicking the function of major histocompatibility complex-restricted, antigen-specific helper T cells. However, IgG1 production was not observed when splenic T cells were added to this system. Thus, self-I-A reactive T cells, generally inactive in the system, may locally help B cell responses to antigens which the T cells do not themselves recognize. In such instances one might attribute to the T cells antigen or idiotype-binding specificities which the cells in reality do not display.
Saito
T
T
Rajewsky
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K
D000818:Animals; D000955:Antigens, T-Independent / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D002999:Clone Cells / Q000276:immunology; D000939:Epitopes / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D007074:Immunoglobulin G / Q000096:biosynthesis; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology* / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000955:Antigens, T-Independent; D000939:Epitopes; D000949:Histocompatibility Antigens Class II; D007074:Immunoglobulin G