Detection of cell-mediated lympholysis determinants encoded by the I-A and I-E genes on ultraviolet-irradiated spleen cells.
We have analysed the effects of ultraviolet (UV) irradiation on the expression, by normal splenic cell populations, of cell-mediated lympholysis determinants encoded by the I-A and I-E genes of the H-2 complex. Cytotoxic T lymphocytes specific for class II antigens could be generated from unprimed responder mice when stimulated with UV-treated cells from I-A-and/or I-E-congenic mice in the presence of interleukin 2-containing medium. A cytotoxic T-cell line specific for I-Ek antigens was obtained and maintained upon stimulation with UV-irradiated spleen cells. Secondary Ia-specific responses were obtained with UV-irradiated stimulators in the absence of added soluble factors. The results show that the failure of UV-irradiated cells to stimulate primary cytotoxic T-cell responses is not due to a selective destruction of class II antigens but rather to the inactivation of a still undefined function of Ia-positive cells, other than interleukin-1 production, involved in the establishment of high-affinity interactions between the responding T helper cells and the stimulator cells.
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Beretta
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Larsson
D000818:Animals; D002478:Cells, Cultured; D000939:Epitopes / Q000235:genetics*; D005802:Genes, MHC Class II; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D007111:Immunity, Cellular; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000166:cytology* / Q000528:radiation effects; D013602:T-Lymphocytes, Cytotoxic / Q000528:radiation effects*; D014466:Ultraviolet Rays
D000939:Epitopes; D000949:Histocompatibility Antigens Class II