Periodate-lysine-paraformaldehyde as fixative for the study of duodenal mucosa. Morphologic and immunohistochemical results at light and electron microscopic levels.
Methods are described which utilize periodate-lysine-paraformaldehyde (PLP) as the only fixative for the investigation of nonfrozen small-bowel biopsy specimens by morphologic and immunohistochemical studies at both light and electron microscopic levels. Small-bowel biopsy specimens were obtained from children suspected of coeliac disease. In light microscopy, there was good morphology and haematoxylineosin staining of the mucosa. Immunofluorescence and immunoperoxidase staining of paraffin-embedded specimens enabled an accurate demonstration of immunoglobulins (IgA, IgM, IgG) in the duodenal mucosa. In conventional electron microscopy, PLP gave a reasonable preservation of ultrastructure. In immunoelectron microscopy, a pre-embedding immunoperoxidase technique provided exact ultrastructural localization of immunoglobulins. The methods are easy to adapt and they give reliable light and electron microscopic results.
I
I
Rantala
M
M
Mäki
A
A
Laasonen
J K
JK
Visakorpi
D002446:Celiac Disease / Q000473:pathology*; D002648:Child; D003176:Complement C3; D004386:Duodenum / Q000473:pathology* / Q000648:ultrastructure; D005404:Fixatives; D005557:Formaldehyde; D006801:Humans; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007158:Immunologic Techniques; D007413:Intestinal Mucosa / Q000473:pathology* / Q000648:ultrastructure; D008239:Lysine; D010504:Periodic Acid; D013194:Staining and Labeling / Q000379:methods
D003176:Complement C3; D005404:Fixatives; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C046311:periodate-lysine-paraformaldehyde; D010504:Periodic Acid; D005557:Formaldehyde; D008239:Lysine