Characteristics of a T lymphocyte-enhancing Ab-specific monoclonal antibody. I. Genetic specificity and preferential enhancement of allogeneic reactions.
We recently described the production and characterization of an Ab-specific monoclonal antibody (mAb), designated K14.83-11, which specifically enhanced the alloreactivity of normal T lymphocytes, as well as highly selected Ab-specific T-cell lines and clones against the appropriate Ab molecule. In the present report, we have studied both the binding specificity and immunoenhancing activity of K14.83-11 in a number of genetic combinations to better characterize the possible modes through which this mAb achieves its T cell-enhancing activity. Results reported here (1) confirm and extend previous findings revealing the possible importance of the immunoglobulin G3 isotype of K14.83-11 in its immunoenhancing activity, and (2) show a distinction in the determinant binding and subsequent ability to elicit an enhancing effect. Surprisingly, K14.83-11 proved ineffective in enhancing autologous or Ab-restricted reactions. This system, then, may provide the means of comparing the functional importance of Ab expression in self-restricted and allogeneic reactions.
A B
AB
Peck
A K
AK
Kimura
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000939:Epitopes; D006133:Growth Substances / Q000096:biosynthesis; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology*; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007141:Immunoglobulin Fc Fragments / Q000276:immunology; D007519:Isoantigens / Q000276:immunology; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000096:biosynthesis; D051379:Mice; D008815:Mice, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006133:Growth Substances; D006183:H-2 Antigens; D007140:Immunoglobulin Fab Fragments; D007141:Immunoglobulin Fc Fragments; D007519:Isoantigens; D008222:Lymphokines