Biochemical and immunological characterization of Suncus murinus mammary tumor virus DNA polymerase.
Enzymological properties of the RNA-directed DNA polymerase associated with the Suncus murinus mammary tumor virus (Sm-MTV) was investigated and its antigenic relatedness to other retroviral DNA polymerases was examined. The enzyme exhibited higher activity in the presence of Mg2+ than in the presence of Mn2+ with endogenous RNA as well as with almost all of the synthetic template X primers tested. Mg2+ was also effective with poly(2'-O-methylcytidylate) X oligodeoxyguanylate which was known to be specific for Mn2+. To examine the immunological relatedness of this enzyme with other retroviral DNA polymerases, remaining Sm-MTV DNA polymerase activity was measured after treatment of this enzyme with various antisera prepared against each of the reverse transcriptases of Mason-Pfizer monkey virus (MPMV), murine mammary tumor virus (MuMTV), simian sarcoma virus-simian sarcoma associated virus (SSV/SSAV), and Rauscher murine leukemia virus (RLV). No inhibition of the Sm-MTV enzyme activity was observed when treated with the latter three antisera with which the DNA polymerase activities of the corresponding retroviruses were fully inhibited. Only the antiserum against MPMV-enzyme, however, was found to slightly inhibit the Sm-MTV enzyme activity. These results indicate that Sm-MTV DNA polymerase has similar enzymological properties to those of MPMV and MuMTV and shares some common antigenic determinant group(s) with MPMV DNA polymerase.
T
T
Yokoi
H
H
Nakane
S
S
Hayakawa
S
S
Saga
S
S
Nagayoshi
Y
Y
Tsutsui
A
A
Aoyama
M
M
Hoshino
K
K
Ono
D000818:Animals; D002460:Cell Line; D008274:Magnesium / Q000378:metabolism; D008321:Mammary Glands, Animal; D008345:Manganese / Q000378:metabolism; D009369:Neoplasms / Q000382:microbiology / Q000662:veterinary; D012194:RNA-Directed DNA Polymerase / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D012190:Retroviridae / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification; D012788:Shrews / Q000382:microbiology; D013379:Substrate Specificity; D014764:Viral Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism
D014764:Viral Proteins; D008345:Manganese; D012194:RNA-Directed DNA Polymerase; D008274:Magnesium