Investigation of antigen cross-reactivity of Mycobacterium leprae-reactive murine T-cell lines and clones.
Inguinal lymph node lymphocytes from BALB/c mice immunized intradermally with 10(8) 60Co-irradiated Mycobacterium leprae were cloned by limiting dilution culture. In general, cloned T-cell lines exhibited helper type activity producing interleukin-2, macrophage activation factor and gamma-interferon and lines were further characterized in terms of their cross-reactivities with other species of mycobacteria. M. leprae clones derived after a period of in vitro restimulation were found to cross-react with other species of mycobacteria probably recognizing non-specific or closely related common cell wall associated mycobacterial determinants. On the other hand, lines established by cloning directly from immune mice appeared more M. leprae-specific, exhibiting antigen-dependent lymphokine production and proliferation in vitro.
A E
AE
Kingston
A J
AJ
Stagg
M J
MJ
Colston
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D002460:Cell Line; D002999:Clone Cells; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2; D008222:Lymphokines; D016215:Macrophage-Activating Factors; D051379:Mice; D008807:Mice, Inbred BALB C; D009166:Mycobacterium leprae / Q000276:immunology*; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology*
D000942:Antigens, Bacterial; D000939:Epitopes; D007376:Interleukin-2; D008222:Lymphokines; D016215:Macrophage-Activating Factors; D007371:Interferon-gamma