Elimination of multicopy plasmid R6K by bleomycin.
Bleomycin eliminated multicopy plasmid R6K from growing cells of Escherichia coli AB1157 but failed to cure either of the low-copy plasmids R1 or R46. Measurements of R6K-encoded beta-lactamase and of covalently closed plasmid DNA indicated that the drug causes a progressive reduction in plasmid copy number.
P V
PV
Attfield
R J
RJ
Pinney
D001761:Bleomycin / Q000494:pharmacology*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000302:isolation & purification; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D011815:R Factors; D001618:beta-Lactamases / Q000378:metabolism
D004269:DNA, Bacterial; D001761:Bleomycin; D001618:beta-Lactamases