Nerve growth factor induces mast cell degranulation without changing intracellular calcium levels.
Nerve growth factor (NGF) induces degranulation of rat peritoneal mast cells (RPMC) in a dose-dependent manner, providing direct evidence for its action on non-neuronal tissues. Activation of RPMC by NGF depends on lysophosphatidylserine and extracellular calcium. NGF-mediated RPMC degranulation is not coupled to a transient increase in intracellular free calcium ([Ca2+]i). It is suggested that NGF has a unique mode of action independent of [Ca2+]i and presumably also without involving protein kinase C activation as indicated by the effects of phorbol esters and NGF on antigen-evoked [Ca2+]i rise.
N
N
Mazurek
G
G
Weskamp
P
P
Erne
U
U
Otten
D000818:Animals; D002118:Calcium / Q000032:analysis*; D004305:Dose-Response Relationship, Drug; D006636:Histamine Release / Q000187:drug effects; D066298:In Vitro Techniques; D008246:Lysophospholipids; D008297:Male; D008407:Mast Cells / Q000032:analysis / Q000187:drug effects*; D009414:Nerve Growth Factors / Q000494:pharmacology*; D010718:Phosphatidylserines / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D008246:Lysophospholipids; D009414:Nerve Growth Factors; D010718:Phosphatidylserines; C025059:lysophosphatidylserine; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium