Modulation of specific protein expression in teratocarcinoma cell aggregates by antibodies affecting cell-cell interactions.
Decompacting Fab were used as tools to modify cell interactions during embryoid body formation and differentiation of the embryonal carcinoma (EC) PSMB. Two kinds of Fab were used: anti-uvomorulin Fab which recognize only the adhesion glycoprotein uvomorulin and Fab from an anti-EC serum which recognizes a series of molecules including uvomorulin. Both types of Fab led to decompaction and formation of loose aggregates with abnormal deposition of extracellular matrix (ECM) components. Anti-uvomorulin Fab did not affect the pattern of embryoid body differentiation. In contrast Fab anti-EC induced a differentiation of most of the cells in the aggregate into an endoderm-like phenotype. This effect was observed only when anti-EC Fab were added to aggregates and not to monolayer cultures.
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D000818:Animals; D000911:Antibodies, Monoclonal; D015820:Cadherins; D002448:Cell Adhesion; D002449:Cell Aggregation; D002450:Cell Communication; D002454:Cell Differentiation; D002460:Cell Line; D003094:Collagen / Q000378:metabolism; D054278:Embryonal Carcinoma Stem Cells; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000276:immunology / Q000502:physiology; D007140:Immunoglobulin Fab Fragments; D007797:Laminin / Q000378:metabolism; D051379:Mice; D014411:Neoplastic Stem Cells / Q000166:cytology* / Q000276:immunology / Q000502:physiology; D013234:Stem Cells / Q000166:cytology*; D000509:alpha-Fetoproteins / Q000378:metabolism
D000911:Antibodies, Monoclonal; D015820:Cadherins; D006023:Glycoproteins; D007140:Immunoglobulin Fab Fragments; D007797:Laminin; D000509:alpha-Fetoproteins; D003094:Collagen