Lectin-binding affinities of the epithelium in the respiratory tract. A light microscopical study of ciliated epithelium in rat, guinea pig, and hamster.
Complex carbohydrate components of surface coat and secretory granules were investigated in the laryngo-tracheo-bronchial epithelium of 3 laboratory animals (rat, guinea pig, and Syrian hamster). 2 groups of epithelial cells were distinguished in the light microscope: ciliated cells and non-ciliated cells. The latter mainly represent secretory cells and are subdivided into serous and mucous secretory cells. Apical glycocalix: In the rat, ciliated cells possess a significant number of Con A, RCA I, and WGA receptors, and a smaller number of UEA I binding sites. In hamsters and in guinea pigs additional binding sites for HPA could be demonstrated. The apical glycocalix of the non-ciliated cells in the rat evince marked staining with RCA I, WGA, and HPA, and less intensive binding of UEA I. In guinea pigs and in hamsters, the presence of additional Con A receptors was noted. Basolateral glycocalix: The basolateral surface coat of ciliated and non-ciliated cells shows identical lectin binding affinities. In the rat, the basolateral glycocalix binds RCA I; in the guinea pig, in addition, positive staining with UEA I and HPA is observed; in the hamster, the basolateral surface coat is outlined by RCA I and HPA receptors. Secretory products: Secretory granules of mucous cells in the rat react with Con A, UEA I and HPA lectins. In guinea pigs, these substances also bind RCA I and WGA lectins. Mucous granules in the secretory cells of the hamster are positive for Con A, RCA I, and HPA lectins. Granules of non-ciliated serous cells of rats bind Con A, UEA I, and HPA lectins. In the guinea pig, this reaction is weaker for UEA I lectin but comparable for Con A and HPA binding. A positive reaction with RCA I lectin only is found in the serous secretory granules of the hamster.
Geleff
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Böck
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Stockinger
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D000423:Alcian Blue; D000818:Animals; D001980:Bronchi / Q000378:metabolism; D002923:Cilia / Q000378:metabolism; D003208:Concanavalin A / Q000378:metabolism; D006224:Cricetinae; D004848:Epithelium / Q000378:metabolism; D005260:Female; D005629:Frozen Sections; D006168:Guinea Pigs; D007830:Larynx / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D008297:Male; D008647:Mesocricetus; D008709:Methenamine; D009092:Mucous Membrane / Q000378:metabolism; D010503:Periodic Acid-Schiff Reaction; D051381:Rats; D011919:Rats, Inbred Strains; D011975:Receptors, Mitogen / Q000032:analysis*; D012137:Respiratory System / Q000378:metabolism*; D012704:Serous Membrane / Q000378:metabolism; D013045:Species Specificity; D013194:Staining and Labeling; D014132:Trachea / Q000378:metabolism; D014909:Wheat Germ Agglutinins
D037102:Lectins; D011975:Receptors, Mitogen; D014909:Wheat Germ Agglutinins; D003208:Concanavalin A; D008709:Methenamine; D000423:Alcian Blue