Clonal growth of bladder cancer cells in a double layer soft agar assay: needles addition of multiple culture supplements.
The necessity of multiple culture supplements, characteristic of Hamburger and Salmon (H&S) soft agar assay (1977), for the clonal growth of bladder cancer cells was systematically investigated. One murine and 6 human cell lines were cultivated in different culture supplemented conditions based on the H&S method. All cell lines successfully formed colonies in the absence of supplements. The inclusion of 2-mercaptoethanol to supplement-free medium stimulated the clonal growth of murine (MBT-2) cells, whereas it did not affect the growth of human cell lines. DEAE-dextran suppressed the clonal growth of 5 out of 7 lines. Tryptic soy broth stimulated the growth of 2 human cell lines, whereas it suppressed the other 2 cell lines. The chemosensitivity of MBT-2 cells was not affected by these supplements.
University of Rochester School of Medicine, Department of Urology, NY.
K
K
Lee
P
P
Smith
G H
GH
Weiss
A T
AT
Cockett
D000818:Animals; D003114:Colony-Forming Units Assay / Q000379:methods*; D003470:Culture Media; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D014407:Tumor Cells, Cultured / Q000166:cytology; D014410:Tumor Stem Cell Assay / Q000379:methods*; D001749:Urinary Bladder Neoplasms
D003470:Culture Media