Determination of glycated hemoglobins of cadaveric blood: a study involving the use of affinity chromatography with m-amino phenyl boronic acid.
For a postmortem diagnosis of diabetes mellitus (DM), the applicability of achieving a determination by examining the glycated hemoglobins (GHb) is discussed. Experimental studies by the use of affinity chromatography with m-amino phenyl boronic acid-agarose revealed that the GHb values could be determined stably and could be reproduced even for some postmortem models of both non-diabetic and diabetic human blood. A subsequent survey of autopsy cases with a proper past DM medical history has suggested that this method is useful, especially when used with customary medicolegal examinations conducted for DM.
Department of Legal Medicine, School of Medicine, Keio University, Tokyo, Japan.
N
N
Kuroda
D000293:Adolescent; D000328:Adult; D000368:Aged; D001897:Boronic Acids; D002102:Cadaver; D002846:Chromatography, Affinity / Q000379:methods; D003920:Diabetes Mellitus / Q000175:diagnosis*; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis*; D006801:Humans; D008297:Male; D008875:Middle Aged
D001897:Boronic Acids; D006442:Glycated Hemoglobin; C028592:3-aminobenzeneboronic acid