Induction of abundant antibody formation with a protein-cellulose complex in mice.
A method is described for immunizing mice with a protein-cellulose complex obtained by covalent coupling of antigenic protein molecules to suspended cellulose particles. Primary immunization of the animals with the complex led to a pronounced immune response persisting for 20-30 days. Subsequent administration of the same antigen in a soluble form resulted in extremely active antibody formation equal to or better than that induced with Freund's complete adjuvant. In BALB/c mice the antibody response was about ten times greater than in the C57BL/6 strain.
Gurvich
A E
AE
Korukova
A
A
D000818:Animals; D000917:Antibody Formation; D000921:Antibody-Producing Cells / Q000276:immunology; D002417:Cattle; D002482:Cellulose / Q000276:immunology; D005620:Freund's Adjuvant / Q000276:immunology; D006736:Horses; D007114:Immunization / Q000379:methods; D007958:Leukocyte Count; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008810:Mice, Inbred C57BL / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology; D013154:Spleen / Q000276:immunology; D005719:gamma-Globulins / Q000276:immunology*
D005719:gamma-Globulins; D002482:Cellulose; D010047:Ovalbumin; D005620:Freund's Adjuvant