Thyroid hormones stimulate the release of hepatic lipase from cultured rat hepatocytes.
We have investigated the effects of triiodothyronine (T3) and thyroxine (T4) on the heparin-stimulated release of hepatic lipase (HL) activity from cultured rat hepatocytes. Addition of T4 (1-10 nmol/l) to the culture medium for 24 h stimulated HL release from cells derived from normal and hypothyroid rats, whereas T3 (0.1-10 nmol/l) was active (at the highest concentration) only in hepatocytes from hypothyroid animals. The effects of T4 could largely be abolished by 5-iodo-2-thiouracil (0.1 mmol/l), an inhibitor of T4-5'-deiodinase. This indicates that the effects of T4 treatment are exerted by T3, formed by deiodination in the hepatocytes.
P
P
Hansson
E
E
Jensen
C H
CH
Florén
P
P
Nilsson-Ehle
D000818:Animals; D013956:Antithyroid Agents / Q000494:pharmacology; D002478:Cells, Cultured; D006493:Heparin / Q000494:pharmacology; D007037:Hypothyroidism / Q000378:metabolism; D008049:Lipase / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013268:Stimulation, Chemical; D013963:Thyroid Hormones / Q000494:pharmacology*; D013974:Thyroxine / Q000494:pharmacology; D014284:Triiodothyronine / Q000494:pharmacology
D013956:Antithyroid Agents; D013963:Thyroid Hormones; D014284:Triiodothyronine; D006493:Heparin; D008049:Lipase; D013974:Thyroxine