Time-series analysis of myoelectric cycling of sphincter of Oddi: evidence of cycling during fed state.
The sphincter of Oddi (SO) in the fasted opossum exhibits spontaneous cyclic spikeburst activity that is temporally related to the interdigestive migrating myoelectric complex (MMC) of the small intestine. After feeding, the cyclic activity is apparently disrupted. Our aim was to use time-series analysis to mathematically describe the periodic behavior of the fasted state and to determine whether this periodicity is present after feeding. Five opossums were chronically prepared with bipolar electrodes secured to the SO. With the animals fasted and conscious, myoelectric activity was recorded for at least two MMC cycles after which they were fed 170 kcal of cream. Spikeburst frequency data were analyzed by fast Fourier transform (FFT) and by digital band-pass filtering. Results from FFT analysis showed an intense low-frequency peak during the fasted state which persisted but was markedly depressed during the fed state. Analysis of the data after filtering also showed persistent sinusoidal activity at the MMC frequency after feeding, although the waveform was significantly depressed by both peak-to-peak amplitude and root mean square amplitude analysis. Recovery to normal fasting cyclic behavior with recovery of both FFT and filtered data was observed. We conclude that in the opossum the cyclic nature of the SO myoelectric activity observed during the fasted state persists after feeding, but is inhibited and masked by an increase in overall spikeburst activity.
Department of Surgery, Washington University School of Medicine, St. Louis, Missouri 63110.
M E
ME
Zenilman
J
J
Parodi
E
E
Spitznagel
J M
JM
Becker
D014670:Ampulla of Vater / Q000502:physiology*; D000818:Animals; D004435:Eating; D004576:Electromyography / Q000379:methods; D005583:Fourier Analysis; D009130:Muscle, Smooth / Q000502:physiology; D009893:Opossums; D010507:Periodicity; D009803:Sphincter of Oddi / Q000502:physiology*; D013997:Time Factors