A monoclonal antibody against mouse oocyte cytoskeleton recognizing cytokeratin-type filaments.
Monoclonal antibodies were raised to detergent-extracted cytoskeleton preparations of mouse oocytes. In immunofluorescence microscopy, one of the antibodies, OCS-1, localizes exclusively to epithelial cells in frozen tissue sections, including various simple and stratified epithelia. The antibody decorates a keratin-type of fibrillar, vinblastine-resistant network in various cultured, epithelial-type cells, but not in myoid or fibroblastoid cells. In mouse oocytes and cleavage-stage embryos, the OCS-1 antibody gives a diffuse, spotty staining pattern. In blastocyst-stage embryos, the antibody reveals a keratin-type filamentous organization in the trophectoderm cells. In immunoelectron microscopy, the OCS-1 antibody decorates 10 nm-thick filaments, often identifiable as desmosome-attached tonofilaments, in detergent-treated trophectoderm cells. The antigen(s) recognized by the OCS-1 antibody is apparently present in, or closely associated with, cytokeratin filaments. In addition to mouse oocytes and early embryos, a wide variety of epithelial cells in various species seem to share this antigen(s). The present results suggest that at the early stages, the cytokeratin-related antigen(s) defined by the OCS-1 antibody are stored in a non-fibrillar form which is then converted into a fibrillar network at the blastocyst stage. A pre-existing supply of cytokeratin-related protein may be essential for the development of the blastocyst.
Lehtonen
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D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis; D001755:Blastocyst / Q000648:ultrastructure; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006825:Hybridomas / Q000276:immunology; D007633:Keratins / Q000276:immunology; D051379:Mice; D008808:Mice, Inbred CBA; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D009865:Oocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000941:Antigens; D007633:Keratins