[Measurement of abundance ratio of 15N in amino acids by capillary gas chromatography-mass spectrometry].
A capillary gas chromatography-mass spectrometry for analysis of 15N-labeled amino acids and amides is described. The method is based on direct silylation of amino acids and amides with MTBSTFA and the formation of the TBDMS derivatives. The method was possible simultaneously to measure the 15N abundance ratio of amino-N and amide-N of amides, as to analysis of amino acids.
Samukawa
K
K
College of Agriculture, University of Osaka Prefecture, Sakai-shi, Japan.
Itano
M
M
Takino
M
M
Komai
Y
Y
D000596:Amino Acids / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D005973:Glutamine / Q000032:analysis; D009587:Nitrogen Isotopes
D000596:Amino Acids; D009587:Nitrogen Isotopes; D005973:Glutamine