Type of lymphocytes affected by the islet-activating protein (IAP).
By flow cytometric analysis, we identified the subclass of lymphocytes that proliferates in response to islet-activating protein (IAP), both in vitro (human peripheral blood mononuclear cells, MNC, cultured with IAP) and in vivo (peripheral blood MNC derived from A/J mice treated with IAP). IAP caused a preferential proliferation of CD8+ T cells. These cells expressed the IL-2 receptors on their surface. CD4+ CD8+ T cells could also be detected in these cultures, IAP caused human MNC to produce IL-1 and to induce expression of HLA-DR antigen. These effects may play an important role in the T-cell proliferation induced by IAP, although IAP by itself suppressed the proliferative action of IL-1 in mouse thymocytes. IAP induced proliferation of the purified CD4+ cells but had a smaller effect on the purified CD8+ cells. This suggests that the proliferation of CD8+ cells in IAP-treated MNC depends on the function of other types of cell, e.g. CD4+ cell and macrophage.
Research and Development Division, Takeda Chemical Industries, Ltd., Osaka, Japan.
Y
Y
Ohta
Y
Y
Nakagawa
T
T
Saijo
D000818:Animals; D015703:Antigens, CD; D006684:HLA-DR Antigens; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000096:biosynthesis; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D037342:Pertussis Toxin; D015375:Receptors, Interleukin-2 / Q000187:drug effects / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D015703:Antigens, CD; D006684:HLA-DR Antigens; D007375:Interleukin-1; D015375:Receptors, Interleukin-2; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin