Response of granulocyte-committed progenitors from patients with chronic myeloid leukemia to humoral regulators release by macrophages in agar diffusion chamber culture.
We investigated the responsiveness of granulocyte-committed progenitors (CFU-G/D) from patients with chronic myeloid leukemia (CML) and healthy subjects to stimulating and inhibiting activities released by murine macrophages in diffusion chamber culture. CFU-G/D from CML demonstrate a normal response to macrophage-derived stimulation. The responsiveness of CFU-G/D from patients with CML to indomethacin-sensitive inhibition was significantly suppressed. In this regard no difference between CFU-G/D from bone marrow and blood of patients with CML could be observed. Colonies formed both by CFU-G/D from healthy subjects and CML consisted exclusively of cells of granulocyte line: from myeloblasts up to polymorphonuclear granulocytes. Similar cellular composition of colonies could be noted during macrophage-derived stimulation and inhibition of CFU-G/D growth. In conclusion, we have demonstrated that CML CFU-G/D which proliferate and differentiate in diffusion chamber culture show a normal response to macrophage-derived stimulation but are less sensitive to indomethacin-dependent inhibition.
J
J
Hansz
K
K
Sawiński
D000328:Adult; D000818:Animals; D002454:Cell Differentiation; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006098:Granulocytes / Q000166:cytology*; D006131:Growth Inhibitors; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007781:Lactoferrin / Q000378:metabolism; D007951:Leukemia, Myeloid / Q000276:immunology*; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D008297:Male; D051379:Mice / Q000276:immunology; D008875:Middle Aged; D014411:Neoplastic Stem Cells / Q000276:immunology*
D006131:Growth Inhibitors; D007781:Lactoferrin; D007213:Indomethacin