Rhodopsin phosphorylation inhibited by adenosine in frog rods: lack of effects on excitation.
The rod photocurrent was studied by recording the transretinal voltage from the aspartate-treated isolated frog retina before and after perfusion with 2 mM adenosine, which inhibited 60-80% of the light-induced rhodopsin phosphorylation. Adenosine did not affect the time courses of the flash photoresponses or the OFF responses after a steady light. The introduction of adenosine while the retina was illuminated by a steady background did not enhance the effect of light. Instead, the opposite change, due to PDE inhibition, was observed. The results indicate that rhodopsin phosphorylation does not determine the time course of the decay of excitation.
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D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000241:Adenosine / Q000494:pharmacology*; D000818:Animals; D003624:Darkness; D007700:Kinetics; D010477:Perfusion; D010766:Phosphorylation; D010775:Photic Stimulation; D010786:Photoreceptor Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D011896:Rana temporaria; D012168:Retinal Pigments / Q000378:metabolism*; D012243:Rhodopsin / Q000378:metabolism*
D012168:Retinal Pigments; D012243:Rhodopsin; D000241:Adenosine; D015056:1-Methyl-3-isobutylxanthine