Differential effect of interferon-gamma and interleukin-2 on the induction of IgA and IgM anti-dextran responses.
Supernatants from T-cell lines and T-cell hybridomas can substitute for T cells in the induction of the anti-alpha(1,3) Dextran B1355 plaque-forming cell response in culture. The present study sought to define the lymphokines required for the induction of IgA and IgM anti-alpha (1,3) dextran responses. Recombinant Interferon-gamma (IFN-gamma) supported the induction of low levels of IgA anti-alpha(1,3) dextran plaque-forming cells in splenic B-cell cultures. IgA responses were substantially increased when cultures containing IFN-gamma were supplemented with an interleukin 2 (IL-2)-containing supernatant from the murine T-cell hybridoma BW.Mls, purified murine IL-2, or recombinant human IL-2. In striking contrast, IgM anti-alpha(1,3) dextran plaque-forming cells were not produced in cultures containing IFN-gamma alone or in combination with purified or recombinant IL-2. However, substantial IgM responses could be produced in cultures containing IFN-gamma and BW.Mls supernatant. This data indicates that there may be different lymphokine requirements for the induction of IgA and IgM anti-alpha(1,3) dextran B cells, or alternatively, such B cells may be in different stages of differentiation and therefore, not respond to the same lymphokines.
P D
PD
Murray
M F
MF
Kagnoff
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D003911:Dextrans / Q000276:immunology*; D004357:Drug Synergism; D006462:Hemolytic Plaque Technique; D007070:Immunoglobulin A / Q000096:biosynthesis*; D007075:Immunoglobulin M / Q000096:biosynthesis*; D007371:Interferon-gamma / Q000494:pharmacology*; D007376:Interleukin-2 / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000494:pharmacology
D003911:Dextrans; D007070:Immunoglobulin A; D007075:Immunoglobulin M; D007376:Interleukin-2; D011994:Recombinant Proteins; D007371:Interferon-gamma