Donor sensitivity to basophil activation by eosinophil granule major basic protein.
Leukocytes of 22 individuals were incubated with a suboptimal concentration of eosinophil granule major basic protein and the amount of histamine release was determined. Cells from 20 of the 22 donors had a histamine release of greater than or equal to 15%. The use of selected donors on repeated occasions revealed significant variability in individual donor responsiveness. The findings support a role for major basic protein activation of human basophils in allergic reactions.
L M
LM
Zheutlin
S J
SJ
Ackerman
G J
GJ
Gleich
L L
LL
Thomas
D001491:Basophils / Q000276:immunology*; D001798:Blood Proteins / Q000276:immunology*; D002478:Cells, Cultured; D047091:Eosinophil Granule Proteins; D004804:Eosinophils / Q000276:immunology*; D006636:Histamine Release; D006801:Humans; D012260:Ribonucleases
D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D012260:Ribonucleases