Characterization of a ribonuclease-sensitive nucleoside triphosphatase activity from HeLa nuclei.
Approximately one-third of the total ATP-hydrolysis activity in isolated HeLa nuclei is sensitive to RNAase (ribonuclease). This activity is selectively extracted with pulse-labelled RNA. In the extracts it co-sediments with various particles with sedimentation coefficients from 10S to 50S, but especially with 24S and 40S particles. ATP hydrolysis by the isolated particles was inhibited extensively (greater than 80%) by RNAase A, heparin and 0.2 M-NaCl. The activity of RNAase-treated particles was recovered when poly(A) was added, but not when DNA was added. The isolated particles exhibited RNAase-sensitive hydrolysis activities for dATP, GTP, CTP and UTP as well as for ATP, and the UTPase activity in the extracts showed nearly the same sedimentation distribution as the ATPase activity. When samples of isolated particles were irradiated with u.v. light in the presence of [alpha-32P]ATP, a 39 kDa polypeptide with a broad distribution from 10S to 50S like that of the ATPase and a 55 kDa polypeptide with a sharp distribution at 24S were photolabelled. Taken together, the data suggest that ATP-hydrolysis activity found in nuclear ribonucleoprotein subfractions appears to be the result of one or two RNA-dependent NTPases that are normally associated with endogenous RNA in a wide variety of particles.
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D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors; D000255:Adenosine Triphosphate / Q000378:metabolism; D002467:Cell Nucleus / Q000201:enzymology; D002499:Centrifugation, Density Gradient; D006367:HeLa Cells / Q000201:enzymology; D006801:Humans; D007700:Kinetics; D043583:Nucleoside-Triphosphatase; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D012260:Ribonucleases / Q000494:pharmacology*; D012261:Ribonucleoproteins / Q000378:metabolism; D014466:Ultraviolet Rays
D012261:Ribonucleoproteins; D012313:RNA; D000255:Adenosine Triphosphate; D012260:Ribonucleases; D010744:Phosphoric Monoester Hydrolases; D000251:Adenosine Triphosphatases; D043583:Nucleoside-Triphosphatase