Fluorometry of poultry semen: its application in the determination of viability, enzyme leakage, and fertility.
The accuracy of fluorometry for estimating percentages of dead chicken spermatozoa was investigated by comparing this technique with the eosin-nigrosin differential staining procedure and with glutamic oxaloacetic transaminase (GOT) concentration in seminal plasma. The relationship between percent dead sperm measured by fluorometry and fertility was also examined. The correlation coefficient of percentage of dead spermatozoa determined by fluorometry with eosin-nigrosin counts was highly significant (r = .99; P less than .001). Similarly, the correlation coefficient of GOT activity with percentage of dead spermatozoa was .99 (P less than .001). Percent fertility, fertile egg production, and duration of fertility were negatively correlated with percent dead spermatozoa; 4 = -.55, -.51, and -.44 (P less than .001), respectively.
S F
SF
Bilgili
J A
JA
Renden
K J
KJ
Sexton
D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism*; D002645:Chickens / Q000502:physiology*; D005298:Fertility; D005470:Fluorometry; D008297:Male; D012661:Semen / Q000502:physiology*; D013094:Spermatozoa / Q000201:enzymology / Q000502:physiology*; D013194:Staining and Labeling / Q000379:methods
D001219:Aspartate Aminotransferases