Localization of the mRNA for the 5-HT2 receptor by in situ hybridization histochemistry. Correlation with the distribution of receptor sites.
32P-labelled oligonucleotides complementary to rat 5-HT2 receptor mRNA were used as probes to study the distribution of cells in rat brain containing the mRNA coding for this receptor by in situ hybridization histochemistry. 5-HT2 receptor binding sites were visualized by autoradiography using [125I]DOI as ligand. Both distributions were comparable, demonstrating that 5-HT2 receptors are expressed by cells intrinsic to the neocortex (lamina Va), claustrum, olfactory bulb and several nuclei of the brainstem.
Preclinical Research, Sandoz Pharma Ltd., Basel, Switzerland.
G
G
Mengod
M
M
Pompeiano
M I
MI
Martínez-Mir
J M
JM
Palacios
D000818:Animals; D001345:Autoradiography; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism*; D007457:Iodine Radioisotopes; D008297:Male; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D011985:Receptors, Serotonin / Q000032:analysis / Q000235:genetics* / Q000378:metabolism
D007457:Iodine Radioisotopes; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D011985:Receptors, Serotonin