Identification of a distal regulatory element in the 5' flanking region of the bovine prolactin gene.
The 5'-flanking region of the bovine prolactin gene was cloned and sequenced. The expression of chimeric gene constructs containing 5'-flanking DNA fragments from the prolactin gene joined to a reporter gene encoding human growth hormone (hGH) was examined using transiently transfected rat pituitary cells. Prolactin nucleotide sequences located at position -1213 to -925 enhance the basal level of expression of growth hormone by 5-fold and function in a position- and orientation-independent fashion. In addition to increasing the basal level of growth hormone expression, this enhancer element also responds to induction by epidermal growth factor. The nucleotide sequence of the bovine prolactin gene enhancer element is highly similar to an enhancer element located approximately -1.5 kb from the rat prolactin transcription initiation site. Deletion analysis of the enhancer region shows that sequences -1124 to -985 are necessary and sufficient for enhancer activity.
BioMolecular Research Department, W.R. Grace and Co-Conn., Washington Research Center, Columbia, MD 21044.
J B
JB
Wolf
V A
VA
David
A H
AH
Deutch
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002478:Cells, Cultured; D002678:Chimera / Q000235:genetics; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D005796:Genes; D013006:Growth Hormone / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D010902:Pituitary Gland / Q000166:cytology; D011388:Prolactin / Q000235:genetics*; D011401:Promoter Regions, Genetic; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D011388:Prolactin; D013006:Growth Hormone