Establishment of a nucleoside-specific suppressor T cell line from SLE prone (NZB/NZW)F1 mice.
A long-term cultured suppressor T cell line (GTS-124) was established from an autoimmune mouse strain, (NZB X NZW)F1, by a two-part procedure: a) B/W F1 mice were made tolerant to guanosine (G) by administration of a tolerogen, the G-modified copolymer of D-glutamic acid and D-lysine (G-D-GL); and b) the spleen cells obtained from tolerant mice were repeatedly stimulated with mitomycin C-treated G-modified syngeneic spleen cells. The GTS-124 cells suppressed the secondary in vitro response to G-keyhole limpet hemocyanin (G-KLH) but did not suppress the response to unrelated antigens, sheep erythrocytes (SRBC), or trinitrophenyl-KLH (TNP-KLH). The expression of Thy-1 antigen on the cell surface of GTS-124 was demonstrated by flow cytometry. Growth of GTS-124 cells was dependent on IL 2. To determine whether GTS-124 cells could suppress the response to nucleosides other than G, KLH coupled with four nucleosides (adenosine [A], G, cytidine [C], and thymine riboside [T]) collectively (AGCT-KLH) was first used as the antigen in the assay system. The PFC response to the individual nucleosides (anti-A, -G, -C, and -T PFC) were effectively inhibited by GTS-124 cells, suggesting that the GTS-124 cells mediated cross-suppression toward all four nucleosides. A more stringent cross-suppression test was conducted by using only the T moiety bound to KLH (T-KLH) as antigen. The results showed that GTS-124 cells were capable of suppressing the T-specific response. The cross-suppression could be seen after repeated selection on a G-BSA-coated dish. These results provide direct evidence that the suppressor T cells induced by in vitro stimulation with G-modified self can indeed suppress the response to nucleosides other than G.
M
M
Naiki
Y
Y
Imai
T
T
Osawa
D000241:Adenosine / Q000276:immunology; D000818:Animals; D000917:Antibody Formation; D002460:Cell Line; D003429:Cross Reactions; D003433:Crosses, Genetic; D003562:Cytidine / Q000276:immunology; D000939:Epitopes; D005260:Female; D006151:Guanosine / Q000276:immunology*; D007108:Immune Tolerance; D008180:Lupus Erythematosus, Systemic / Q000235:genetics / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008814:Mice, Inbred NZB; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D013936:Thymidine / Q000276:immunology; D013997:Time Factors
D000939:Epitopes; D006151:Guanosine; D003562:Cytidine; D000241:Adenosine; D013936:Thymidine