Isolation of a novel cell-attachment and spreading-promoting protein from human serum.
A protein with potent cell-attachment and spreading-promoting activity was isolated from fibronectin-free human serum. The purification steps included affinity chromatography on heparin-agarose and preparative isoelectric focusing. The purified protein was homogeneous as judged from dodecyl sulphate/polyacrylamide-gel electrophoresis. It had an isoelectric point of 5.0 and an Mr of 52 000. The protein promoted the spreading of Chinese-hamster ovary cells to plastic in a manner similar to that observed with fibronectin.
Vuento
M
M
Korkolainen
M
M
Kuusela
P
P
Hölttä
E
E
D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification*; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D002846:Chromatography, Affinity; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006023:Glycoproteins / Q000302:isolation & purification* / Q000494:pharmacology; D007525:Isoelectric Focusing; D010053:Ovary / Q000166:cytology; D019096:Vitronectin
D001798:Blood Proteins; D006023:Glycoproteins; D019096:Vitronectin