Bleomycin selectively elevates mRNA levels for procollagen and fibronectin following acute lung injury.
We employed the technique of dot blot hydridization of radiolabeled cDNA probes to examine the role of specific mRNA content in the control of extracellular matrix turnover in the remodeling rat lung. Following bleomycin instillation, total RNA content gradually doubled during the first 5 days following the initial lung injury, then rose much more rapidly during the ensuing 9 days. Individual mRNAs for procollagens I and III and for fibronectin were selectively enriched 2- to 4-fold above total RNA during the first week after bleomycin instillation. No comparable increases were observed in specific RNAs from liver, indicating that the response observed in the lung was not generalized to other organs. Moreover, the increases in mRNA species for procollagen types I or III in the lung could not be related to the influx of inflammatory cells which migrate into the lungs during acute injury, as cells obtained by alveolar lavage contained no mRNAs for procollagens.
J
J
Kelley
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L
Chrin
S
S
Shull
D W
DW
Rowe
K R
KR
Cutroneo
D000818:Animals; D001761:Bleomycin / Q000494:pharmacology*; D004274:DNA, Recombinant; D005109:Extracellular Matrix / Q000378:metabolism; D005353:Fibronectins / Q000235:genetics*; D007700:Kinetics; D008171:Lung Diseases / Q000139:chemically induced / Q000378:metabolism*; D008297:Male; D009693:Nucleic Acid Hybridization; D011347:Procollagen / Q000235:genetics*; D011658:Pulmonary Fibrosis / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344
D004274:DNA, Recombinant; D005353:Fibronectins; D011347:Procollagen; D012333:RNA, Messenger; D001761:Bleomycin; D012313:RNA