[Immunoglobulins acquire the ability to interact with DNA after chromatography on QAE-Sephadex].
The composition of IgG isolated from normal human serum includes a significant amount of antibodies interacting with native DNA. This ability of antibodies is revealed only after anion-exchange chromatography, resulting in the division of IgG into two fractions, i.e., acid and alkaline immunoglobulins. The peculiarities of interaction of the both fractions with DNA and the specificity of this reaction were investigated. The IgG tested were shown to interact with native and denaturated DNA, dextran sulfate, poly-G and poly-I. The possibility of interaction of antibodies with the charged structures of the cell and their significance under normal and pathological conditions are discussed.
Sulaeva
N I
NI
Lekakh
I V
IV
Poverennyĭ
A M
AM
D000974:Antibodies, Antinuclear / Q000032:analysis; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D002852:Chromatography, Ion Exchange; D004247:DNA / Q000276:immunology*; D003911:Dextrans; D006801:Humans; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology; D066298:In Vitro Techniques
D000974:Antibodies, Antinuclear; D000911:Antibodies, Monoclonal; D003911:Dextrans; D007074:Immunoglobulin G; D004247:DNA; C025614:sephadex
Immunoglobuliny priobretaiut sposobnost' vzaimodeĭstvovat' s DNK posle khromatografirovaniia na QAE-sefadekse.