Strand specific transcription of satellite DNA I in rat ascites hepatoma cells.
Rat satellite DNA I was highly expressed in a rat ascites hepatoma cell line, AH60C. The transcripts were found predominantly in the non-polyadenylated fraction of the nuclear RNA. RNA dot blot assay using strand specific probes revealed a strand-selective transcription of the sequence. The structures of cDNA clones isolated on the basis of their cross-hybridization to satellite DNA I were mainly composed of a tandemly repeated array of this sequence.
M
M
Miyahara
H
H
Sumiyoshi
M
M
Yamamoto
H
H
Endo
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000032:analysis; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004276:DNA, Satellite / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D005786:Gene Expression Regulation; D008114:Liver Neoplasms, Experimental / Q000235:genetics*; D011061:Poly A / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic
D004276:DNA, Satellite; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA; D004262:DNA Restriction Enzymes