Evaluation of isolator system and large-volume centrifugation method for culturing body fluids.
The Isolator system was compared with the large-volume centrifugation method for processing and recovering organisms from body fluids other than blood, cerebrospinal fluid, and urine. A total of 155 body fluid samples were processed for the recovery of clinically significant organisms. Of the 55 positive cultures, Isolator detected 94% and the large-volume centrifugation method detected 64%. The time necessary to indicate positivity was not significantly different in the two methods; however, in five cases, the Isolator system yielded clinically significant organisms 24 h sooner than the conventional method. The Isolator system was found to be a more sensitive alternative than the conventional large-volume centrifugation method.
Clinical Microbiology Laboratory, Veterans Administration Hospital, Loma Linda, California 92357.
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Elston
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Wang
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Philip
D001419:Bacteria / Q000302:isolation & purification; D001826:Body Fluids / Q000382:microbiology*; D002498:Centrifugation; D005069:Evaluation Studies as Topic; D006801:Humans; D008828:Microbiological Techniques