Rapid induction of nucleoside-diphosphate kinase in HeLa S3 cells by human-type interferons.
Nucleoside-diphosphate (NDP)-kinase can be considered to be induced by human-type interferons (HuIFNs) rapidly, since an enzyme increase was detected within 2 h of incubation of HeLa S3 cells with HuIFNs, while incubation with heterologous mouse IFNs had no such effect. The enzyme increase induced by HuIFNs reached a plateau at 6 h after treatment. Actinomycin D (0.5 microgram/ml) significantly blocked the enzyme increase induced by HuIFNs in the cells. A possible biological role of the enzyme in the IFN-induced biochemical events is discussed.
K
K
Ohtsuki
M
M
Yokoyama
T
T
Ikeuchi
N
N
Ishida
K
K
Sugita
K
K
Satoh
D000244:Adenosine Diphosphate / Q000096:biosynthesis; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D006367:HeLa Cells / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D007739:L Cells / Q000187:drug effects / Q000201:enzymology; D051379:Mice; D009701:Nucleoside-Diphosphate Kinase / Q000096:biosynthesis*; D010766:Phosphorylation; D010770:Phosphotransferases / Q000096:biosynthesis*; D013045:Species Specificity
D003609:Dactinomycin; D000244:Adenosine Diphosphate; D007372:Interferons; D010770:Phosphotransferases; D009701:Nucleoside-Diphosphate Kinase; D002118:Calcium