[Determination of estrogen receptors in 160 breast tumors using monoclonal antibodies: comparison of Abbott's immunoenzymatic method with the carbon dextran method].
Estrogen receptor determinations were performed on 241 cytosols from 160 breast cancer tumors, using both dextran coated charcoal method (D.C.C.) and monoclonal antibodies (Abbott's ER-EIA kit) in order to compare the two methods, and to evaluate the clinical usefulness of this new immunological simplified assay. Intra- and inter-assay reproducibility were studied during a six month period. Intra-assay of both methods were lower than 5%. Inter-assay variation coefficients of ER-EIA studied on 35 standard curves varied from 12.5% (standard: 0) to 6.7% (standard: 250 fmoles/ml). ER determination of 80 human breast cancers were performed both by EIA and Scatchard analysis. The regression curve obtained was (EIA) = 1.04 (Scatchard) + 21 fmoles/mg of proteins (r = 0.963). With 153 breast cancer cytosols whose volume was too small for multipoint Scatchard analysis, EIA results were compared to results obtained by a "near saturating" concentration of tritiated ligand (5 nM). The regression curve obtained was (EIA) = 1.34 (5nM) + 5 fmoles/mg proteins (r = 0.978). These results can be compared with those obtained between Scatchard and 5 nM: (Scatchard) = 1.29 (5nM) - 9 fmoles/mg proteins (r = 0.985). Reproducibility was studied on clinical specimens assayed at two different periods during the clinical evaluation. Regression curves obtained were (2nd assay) = 1.05 (1st assay) - 5.5 fmoles/mg proteins for EIA, and (2nd assay) = 0.96 (1st assay) + 9 fmoles/mg proteins for DCC method. EIA assay presented an high stability for protein concentrations very low, up to 0.2 mg/ml. Finally, a very good correlation was obtained between ER-EIA and DCC method, and ER-EIA seemed specially well fitted to small tumors.
Goussard
J
J
Lechevrel
C
C
Martin
P M
PM
Roussel
G
G
D000328:Adult; D000911:Antibodies, Monoclonal; D001943:Breast Neoplasms / Q000032:analysis*; D002244:Carbon; D003429:Cross Reactions; D003911:Dextrans; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D011869:Radioligand Assay; D011960:Receptors, Estrogen / Q000032:analysis*; D011980:Receptors, Progesterone / Q000032:analysis
D000911:Antibodies, Monoclonal; D003911:Dextrans; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D002244:Carbon
Détermination des récepteurs d'oestrogènes par anticorps monoclonaux dans 160 tumeurs mammaires: comparaison de la méthode immunoenzymatique Abbott avec la méthode au charbon dextran.