The redox state of activated bleomycin.
Activated bleomycin appears to have two more oxidizing equivalents than the Fe(III).bleomycin to which it spontaneously decays. Activated bleomycin reacts with NADH and thio-NADH, two-electron reductants, and with KI, a one-electron reductant, to yield Fe(III).bleomycin. The observed stoichiometries were 0.85 +/- 0.07 eq of thio-NADH oxidized or 1.5 +/- 0.25 eq of KI oxidized per mole of activated bleomycin. None of these reactions requires the presence of a redox mediator, as does the reduction of Fe(III).bleomycin by NADH or thio-NADH. The oxidations of both pyridine nucleotide coenzymes and of KI are inhibited by DNA, the usual bleomycin target.
R M
RM
Burger
J S
JS
Blanchard
S B
SB
Horwitz
J
J
Peisach
D001761:Bleomycin; D055598:Chemical Phenomena; D002621:Chemistry; D004578:Electron Spin Resonance Spectroscopy; D008773:Methylphenazonium Methosulfate / Q000494:pharmacology; D009243:NAD / Q000031:analogs & derivatives / Q000378:metabolism; D010084:Oxidation-Reduction; D013997:Time Factors
D009243:NAD; D001761:Bleomycin; D008773:Methylphenazonium Methosulfate; C003451:thionicotinamide adenine dinucleotide