Experimental model of immune-mediated hearing loss using cross-species immunization.
The presence of immune-mediated hearing loss was investigated in an animal model. Eight guinea pigs and four mice underwent immunizations with a preparation of chick or guinea pig cochlear tissue and Freund's adjuvant. Hearing thresholds were monitored by auditory brainstem response (ABR) testing over a 5-week period after immunization. The serum and temporal bones of test and control animals were then examined using an enzyme-linked immunosorbent assay (ELISA), immunocytochemical, and histological techniques. Hearing loss of 20 dB or greater occurred in eight animals. ELISA demonstrated antibodies to cochlear antigens in the sera of all test animals. Immunocytochemistry revealed immunostaining of hair cell stereocilia in the organ of Corti and saccule. Endolymphatic hydrops, and organ of Corti degeneration was observed in the temporal bones of three animals. This study provides evidence to suggest that cross-species immunization with cochlear antigens might produce a humoral response that can be associated with inner ear pathologic change and sensorineural hearing loss.
Department of Otolaryngology-Head and Neck Surgery, University of Michigan Hospitals, Ann Arbor.
C R
CR
Orozco
J K
JK
Niparko
B C
BC
Richardson
D F
DF
Dolan
M U
MU
Ptok
R A
RA
Altschuler
D000818:Animals; D000917:Antibody Formation; D001300:Audiometry, Evoked Response; D001327:Autoimmune Diseases / Q000150:complications* / Q000473:pathology / Q000503:physiopathology; D002645:Chickens; D003051:Cochlea / Q000276:immunology; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005620:Freund's Adjuvant / Q000276:immunology; D006168:Guinea Pigs; D006319:Hearing Loss, Sensorineural / Q000209:etiology* / Q000276:immunology / Q000473:pathology / Q000503:physiopathology; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D013045:Species Specificity; D013701:Temporal Bone / Q000473:pathology
D005620:Freund's Adjuvant