Sequencing studies of ICR-170 mutagenic specificity in the am (NADP-specific glutamate dehydrogenase) gene of Neurospora crassa.
The acridine half-mustard ICR-170-induced reversion of the mutant am15, which has a single base-pair deletion, at a frequency of between 9 and 28 X 10(-6). In each of three classes of revertants, the mutagen had induced the insertion of a -G- -C- base pair at a -G-G- -C-C- site. The mutant am6, which has a single base pair insertion, is known to be revertible, with UV light, by deletion of a -G- -C- base pair at a -G-G-G- -C-C-C- site. This mutant reverted with ICR-170 at a frequency of 0.1 X 10(-6). These results show that ICR-170 is able to induce addition frameshifts in Neurospora crassa within short, monotonous runs of G:C base pairs, but indicate a lack of deletion activity at such sequences.
P A
PA
Burns
J H
JH
Kinnaird
B J
BJ
Kilbey
J R
JR
Fincham
D000595:Amino Acid Sequence; D000609:Aminoacridines; D001482:Base Composition; D001483:Base Sequence; D005796:Genes; D005800:Genes, Fungal; D005969:Glutamate Dehydrogenase / Q000235:genetics*; D009154:Mutation; D009491:Neurospora / Q000235:genetics*; D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics*; D009588:Nitrogen Mustard Compounds / Q000494:pharmacology*
D000609:Aminoacridines; D009588:Nitrogen Mustard Compounds; C091910:acridine mustard; D005969:Glutamate Dehydrogenase