Cysteamine effects on somatostatin, catecholamines, pineal NAT and melatonin in rats.
The thiol reagent cysteamine was administered to adult male rats with the aim of investigating its effect on different neural and pineal components. As expected, immunoreactive somatostatin decreased in the median eminence (ME) (p less than 0.05) and gastric antrum (p less than 0.05) after cysteamine; however, no significant change was observed in the pineal IRS content after drug treatment. A decrease in norepinephrine was observed in the ME (p less than 0.001), hypothalamus (p less than 0.001) and pineal gland (p less than 0.05), together with a rise in ME (p less than 0.005) and hypothalamic dopamine (p less than 0.005) content; these results are consistent with a dopamine-beta-hydroxylase inhibiting effect of cysteamine. No effect was observed on hypothalamic serotonin and 5-hydroxyindole-acetic acid content. Pineal N-acetyltransferase (NAT) activity was significantly higher (p less than 0.05) after cysteamine than after saline, but no statistically significant effect was observed on pineal melatonin content. The mechanism involved in the NAT rise is presumably not related to the known stimulatory effect of norepinephrine, which fell after cysteamine. It is suggested that cysteamine may act at an intracellular level, inhibiting NAT degradation, an effect demonstrated in vitro and thought to be related to a thiol:disulfide exchange mechanism.
S M
SM
Webb
T H
TH
Champney
R W
RW
Steger
M K
MK
Vaughan
R J
RJ
Reiter
D000123:Acetyltransferases / Q000378:metabolism*; D000818:Animals; D002395:Catecholamines / Q000378:metabolism*; D003543:Cysteamine / Q000494:pharmacology*; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism; D008297:Male; D008473:Median Eminence / Q000378:metabolism; D008550:Melatonin / Q000378:metabolism*; D009994:Osmolar Concentration; D010870:Pineal Gland / Q000378:metabolism*; D011706:Pyloric Antrum / Q000378:metabolism; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000378:metabolism; D013004:Somatostatin / Q000378:metabolism*
D002395:Catecholamines; D012701:Serotonin; D013004:Somatostatin; D006897:Hydroxyindoleacetic Acid; D003543:Cysteamine; D000123:Acetyltransferases; D008550:Melatonin