Induction of error-free DNA repair in Escherichia coli by thiamine deprivation.
Incubation of Escherichia coli AB1157 in a thiamine-deficient medium causes a large, time-dependent increase in resistance to UV-radiation (254 nm) and a fall in its UV-induced mutation frequency to histidine prototrophy which are abolished in its uvrA mutant, but only delayed in lon- and recA- cells. The response of the lexA3 mutant resembles that of the parental cells. These effects are very similar to those we have shown to be induced by heat shock and are clearly due to an error-free, DNA-excision repair-dependent process. They may represent a general response to non-mutagenic stress in these cells.
Department of Biochemistry, University of Ottawa, Ont., Canada.
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Fitt
D001426:Bacterial Proteins / Q000235:genetics; D004260:DNA Repair; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects; D005798:Genes, Bacterial; D006358:Hot Temperature; D009154:Mutation; D013014:SOS Response, Genetics; D013831:Thiamine / Q000378:metabolism*; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D013831:Thiamine