Development of ionic channels during mouse neuronal differentiation.
Using a mouse embryonal teratocarcinoma (E.C.) cell line, it was possible to follow the sequence of development of ionic channels during neuronal differentiation, with patch-clamp techniques. 1003 E.C. cells were induced to differentiate into neurons by culturing them in defined medium without foetal calf serum (DARMON et al., 1981). Non-differentiated cells were not excitable and presented mainly 2 types of K+ channels: a Ca2+ activated K+ channel (220 pS in symmetrical K+) and a delayed rectifier (30 pS in symmetrical K+). When the cells start to grow neurites, a low threshold calcium current can be recorded, only if the cell is held at hyperpolarized potentials (-70 to -80 mV). Fully differentiated cells with long neurites presented a complete repertoire of ionic channels: voltage dependent Na+ and Ca2+ channels, Ca2+ activated K+ channel and K+ delayed rectifier.
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M
Simonneau
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C
Distasi
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L
Tauc
C
C
Poujeol
D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000502:physiology; D002454:Cell Differentiation; D002999:Clone Cells; D007473:Ion Channels / Q000502:physiology*; D051379:Mice; D009474:Neurons / Q000502:physiology*; D010641:Phenotype; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D013724:Teratoma
D007473:Ion Channels; D012964:Sodium; D011188:Potassium; D002118:Calcium