In vitro production of pregnancy-associated plasma protein-A (PAPP-A) by trophoblastic cells.
Pregnancy-associated plasma protein-A (PAPP-A) a high molecular weight glycoprotein, is found in high concentration in the maternal circulation during pregnancy. Immunohistochemically, PAPP-A can be localized in the trophoblast and in the decidua. Short term cultures of trophoblastic and decidual explants produce PAPP-A in vitro. It was thus of interest to see if long term cultures of primary monolayers of human trophoblast cells were capable of producing PAPP-A. Under our in vitro conditions, trophoblastic monolayers were producing both PAPP-A and beta hCG. During the first 3 days of culture PAPP-A levels increased in the medium whereas beta hCG levels decreased. The production of both proteins could be inhibited by cycloheximide. These results strongly suggest that the trophoblast is a source of PAPP-A in vivo.
Barnea
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ER
Sanyal
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MK
Brami
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C
Bischof
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P
D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000096:biosynthesis; D003513:Cycloheximide / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D011257:Pregnancy Proteins / Q000096:biosynthesis*; D011266:Pregnancy-Associated Plasma Protein-A / Q000096:biosynthesis*; D012636:Secretory Rate / Q000187:drug effects; D014327:Trophoblasts / Q000166:cytology / Q000378:metabolism*
D006063:Chorionic Gonadotropin; D011257:Pregnancy Proteins; D003513:Cycloheximide; D011266:Pregnancy-Associated Plasma Protein-A