Immunocytochemical evidence for synaptic regulation of paraventricular vasopressin-containing neurons by substance P.
Employing a combination of pre-embedding peroxidase-antiperoxidase-labeling for substance P (SP) and postembedding immunogold labeling with protein A-colloidal gold-anti-arginine vasopressin (AVP) complex, we demonstrated immunoreactive SP containing nerve fibers, which terminate synaptically on the perikarya, contained gold-labeled secretory granules in the magnocellular paraventricular nucleus of rats. The perikarya were also synapsed with unlabeled nerve fibers. It is concluded that SP plays a role as an axosomatic neurotransmitter in diverse synaptic controls of vasopressinergic neurons.
Heike
Y
Y
Hisano
S
S
Tsuruo
Y
Y
Katoh
S
S
Daikoku
S
S
D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D008297:Male; D008854:Microscopy, Electron; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013373:Substance P / Q000378:metabolism*; D013569:Synapses / Q000378:metabolism
D001127:Arginine Vasopressin; D013373:Substance P