Interleukin 2-dependent release of interleukin 3 activity by T4+ human T-cell clones.
We have isolated and studied two alloreactive, T4+, human lymphocyte clones that release interleukin 2 (IL-2) and interleukin 3 (IL-3) bioactivities upon stimulation with IL-2, alloantigen, or Sepharose-conjugated antibodies directed against the T3 protein. Anti-IL-2 receptor monoclonal antibodies block IL-2-, alloantigen-, or anti-T3-stimulated IL-3 release. Hence, release of IL-3 activity in each circumstance is rigorously dependent upon activation of the IL-2 receptor. Even low, nonmitogenic concentrations of recombinant IL-2 stimulated IL-3 release.
A A
AA
Ythier
M
M
Abbud-Filho
J M
JM
Williams
R
R
Loertscher
M W
MW
Schuster
A
A
Nowill
J A
JA
Hansen
D
D
Maltezos
T B
TB
Strom
D002478:Cells, Cultured; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic; D004261:DNA Replication; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D007376:Interleukin-2 / Q000276:immunology*; D007377:Interleukin-3; D008222:Lymphokines / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D007376:Interleukin-2; D007377:Interleukin-3; D008222:Lymphokines