Neutron radiation can activate K-ras via a point mutation in codon 146 and induces a different spectrum of ras mutations than does gamma radiation.
Neutron radiation is known to produce tumors in animals and cause cell transformation. We have developed a protocol to efficiently induce thymic lymphomas in RF/J mice by a single acute dose of neutron irradiation. Activated ras genes were detected in 17% (4 of 24) of the tumors analyzed. One of the tumors contained a K-ras gene activated by a point mutation in codon 146. Activating ras mutations at position 146 have not been previously detected in any known human or animal tumors. The spectrum of ras mutations detected in neutron radiation-induced thymic lymphomas was different from that seen in thymic lymphomas induced by gamma radiation in the same strain of mice. These results may have important implications for the mechanisms by which different types of radiation damage DNA.
Department of Pathology, New York University School of Medicine, New York 10016.
S R
SR
Sloan
E W
EW
Newcomb
A
A
Pellicer
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000528:radiation effects; D005720:Gamma Rays; D005784:Gene Amplification; D011905:Genes, ras; D008223:Lymphoma / Q000235:genetics; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D009154:Mutation; D009381:Neoplasms, Radiation-Induced / Q000235:genetics; D009502:Neutrons; D015689:Oncogene Protein p21(ras) / Q000235:genetics*; D011519:Proto-Oncogenes / Q000528:radiation effects*
D004247:DNA; D015689:Oncogene Protein p21(ras)