A monoclonal antibody recognizing a determinant shared by HLA-A2 and HLA-Aw69 (A28* variant).
A cytotoxic murine monoclonal antibody, designated P5.1, was tested against 613 unrelated donors and found to react with 401 who were positive for HLA-A2 (sensitivity = 100%) and with 8 of 82 positive for HLA-A28. The latter split of A28 corresponds to the "A28* variant" that in the Ninth International Histocompatibility Workshop (9WS) was designated Aw69(28*). The epitope recognized by antibody P5.1 is distinct from the alloantisera-defined determinants that characterize HLA-A2 and A28. Immunoprecipitation of specific antigens with selected monoclonal antibodies and isoelectric focusing gel electrophoresis demonstrated that A2, Aw68(28) and Aw69(28*) are distinct polypeptides. Thus, the A2-A28 antigen family consists of at least three different alleles definable using alloantiserums specific for A2 and A28, and monoclonal antibodies such as P5.1 recognizing the A2,Aw-69(28*)-epitope.
P
P
Antonelli
S Y
SY
Choo
B
B
Nisperos
J A
JA
Hansen
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000939:Epitopes; D006680:HLA Antigens / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D015234:HLA-A Antigens; D015789:HLA-A2 Antigen; D006801:Humans; D007526:Isoelectric Point; D010375:Pedigree
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006680:HLA Antigens; D015234:HLA-A Antigens; D015789:HLA-A2 Antigen; C037164:HLA-A28 antigen