In vitro diffusion in polyacrylamide embedded agarose microbeads.
125I Sodium iodide, 125I insulin, 125I albumin, and 111indium IGG were employed to investigate release from, and penetration of different sized molecules into agarose/polyacrylamide microcapsules. The microcapsules were formed by photopolymerization of an acrylamide solution round agarose beads. The indium-chelated antibody gave a particular low background count. The different release times were explained in terms of differences in diffusion coefficient. By retarding in vitro penetration of antibodies, these microcapsules could be of value for the encapsulation of living cells in bioartificial organs.
INSERM U 306, Bordeaux, France.
H
H
Gin
B
B
Dupuy
J
J
Caix
C
C
Baquey
D
D
Ducassou
D000180:Acrylic Resins; D000418:Albumins; D000911:Antibodies, Monoclonal; D002214:Capsules; D004058:Diffusion; D007328:Insulin; D010539:Permeability; D012685:Sepharose; D012974:Sodium Iodide; D000099166:Polyacrylamides
D000180:Acrylic Resins; D000418:Albumins; D000911:Antibodies, Monoclonal; D002214:Capsules; D007328:Insulin; D012685:Sepharose; D012974:Sodium Iodide; D000099166:Polyacrylamides