High susceptibility to ADP-induced thrombus formation in mast cell-deficient W/Wv mice.
We used mast cell-deficient W/Wv mice to clarify whether mast cell-derived heparin may play a role in inhibiting thrombus formation in the living organism. Small veins in the mesentery of W/Wv or congenic +/+ mice were stretched over an inverted microscope; a micropipette filled with varying concentrations of ADP was set close to the outside of a vein by using a micromanipulator. Thrombus formation was directly examined under the microscope. The concentration of ADP necessary for thrombus formation was significantly lower in the W/Wv mice than in the congenic +/+ mice. Furthermore, the concentration of ADP necessary for aggregation of platelets in platelet-rich plasma (PRP) was significantly lower in W/Wv mice than in +/+ mice. The higher sensitivity of PRP of W/Wv mice is not attributed to the platelets, but to the plasma, since platelets of +/+ mice suspended in platelet-poor plasma (PPP) of W/Wv mice were more sensitive to ADP than platelets of W/Wv mice suspended in PPP of +/+ mice. The present results suggest that plasma of W/Wv mice may lack any inhibitory factor(s) or contain promoting factor(s) for platelet aggregation.
K
K
Hatanaka
M
M
Minamiyama
K
K
Tanaka
T
T
Taguchi
S
S
Tajima
Y
Y
Kitamura
A
A
Yamamoto
D000244:Adenosine Diphosphate; D000818:Animals; D001777:Blood Coagulation; D000939:Epitopes / Q000032:analysis; D005340:Fibrinogen / Q000032:analysis / Q000276:immunology; D006801:Humans; D008297:Male; D008407:Mast Cells / Q000502:physiology*; D008642:Mesenteric Veins / Q000503:physiopathology*; D051379:Mice; D008817:Mice, Mutant Strains; D010974:Platelet Aggregation; D010976:Platelet Count; D013927:Thrombosis / Q000097:blood / Q000139:chemically induced / Q000503:physiopathology*
D000939:Epitopes; D000244:Adenosine Diphosphate; D005340:Fibrinogen