Inhibition of phytohemagglutinin-induced DNA synthesis and of adenosine deamination by 5-azacytidine and 5-aza-2'-deoxycytidine in intact human cells.
5-Azacytosine nucleosides block phytohemagglutinin-induced thymidine incorporation into DNA in intact human blood lymphocytes in a dose-dependent manner at 1-100 micrograms/ml. Maximal inhibition was observed when mitogen and 5-azacytidine or 5-aza-2'-deoxycytidine were added to the culture in admixture. Both analogues also inhibit deamination of exogenous adenosine in stimulated cells without affecting the synthesis of nucleotides from adenosine, adenine or hypoxanthine. Similar inhibitory effect of 5-aza-2'-deoxycytidine on the metabolism of adenosine was observed in normal (WI-38) and SV40 virus-transformed (VA-13) human fibroblasts.
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D000241:Adenosine / Q000378:metabolism*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001374:Azacitidine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D003641:Deamination; D000077209:Decitabine; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D010835:Phytohemagglutinins / Q000494:pharmacology*
D000970:Antineoplastic Agents; D010835:Phytohemagglutinins; D000077209:Decitabine; D004247:DNA; D000241:Adenosine; D001374:Azacitidine