Preparation and properties of antibodies doubly labeled with tritium and fluorescein.
A method was developed to prepare doubly labeled antibodies whereby fluorescein-labeled antibodies were reacted with N-[ethyl-2-3H]ethylmaleimide. This tritiated conjugate exhibited the same immunoreactivity as the non-derivatized fluorescein-labeled antibody. This method provides a marker for immunocytochemical studies which takes advantage of the rapidity of fluorescent tracing combined with the stability and precision of the radioautography technique.
M C
MC
Léveillé
K D
KD
Roberts
A
A
Chapdelaine
S
S
Chevalier
G
G
Bleau
D000818:Animals; D000937:Antigen-Antibody Reactions; D001345:Autoradiography; D006224:Cricetinae; D005033:Ethylmaleimide; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G; D051379:Mice; D010063:Ovum / Q000276:immunology; D011233:Precipitin Tests; D013194:Staining and Labeling; D013861:Thiocyanates; D014316:Tritium
D005452:Fluoresceins; D007074:Immunoglobulin G; D013861:Thiocyanates; D014316:Tritium; D016650:Fluorescein-5-isothiocyanate; D005033:Ethylmaleimide