Rapid detection of respiratory syncytial virus antigens in nasopharyngeal secretions.
The combined use of fluorescein-labelled monoclonal antibody and a cytocentrifuge for preparation of cell spots greatly reduced the time for rapid diagnosis, and improved the sensitivity and ease of detection of respiratory syncytial (RS) virus antigen in specimens of nasopharyngeal secretions.
Regional Virus Laboratory, City Hospital, Edinburgh, U.K.
H A
HA
Cubie
G F
GF
Winter
E E
EE
Leslie
J M
JM
Inglis
D000956:Antigens, Viral / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D009302:Nasopharyngeal Diseases / Q000175:diagnosis / Q000382:microbiology*; D012136:Respiratory Syncytial Viruses / Q000276:immunology*; D010253:Respirovirus Infections / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D013997:Time Factors
D000956:Antigens, Viral