Cell spreading on laminin substrate involves Con A-binding proteins.
Concanavalin A (Con A), a tetravalent lectin, was shown to impair 8 chick embryo fibroblast (8 d CEF) spreading on a laminin (LM) substrate but not on a fibronectin substrate (FN), suggesting that cell surface Con A binding proteins could be involved in 8 d CEF spreading on a LM substrate. The interaction of Con A-binding proteins with Con A is dependent upon the carbohydrate moieties of the isolated glycoproteins; since they interact strongly with Con A-Sepharose and are eluted with 0.3 Mol/l alpha-methylmannopyranoside, the isolated Con A binding-proteins inhibit 8 d CEF adhesion to a Con A substrate to the same extent as alpha-methylmannopyranoside. Furthermore, the isolated Con A binding proteins specifically inhibit in a dose-dependent manner 8 d CEF spreading on LM but not on FN.
INSERM U. 180, UFR Biomédicale des Saints-Péres, Paris, France.
R
R
Moutsita
J
J
Botti
M A
MA
Doyennette-Moyne
M
M
Aubery
P
P
Codogno
D000818:Animals; D001665:Binding Sites; D002448:Cell Adhesion / Q000187:drug effects; D002642:Chick Embryo; D003208:Concanavalin A / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D006023:Glycoproteins / Q000502:physiology; D007797:Laminin; D008766:Methylmannosides / Q000494:pharmacology; D011952:Receptors, Concanavalin A / Q000494:pharmacology / Q000502:physiology*
D006023:Glycoproteins; D007797:Laminin; D008766:Methylmannosides; D011952:Receptors, Concanavalin A; C027774:concanavalin A-binding glycoproteins; D003208:Concanavalin A; C008466:methylmannoside