[Suppression of the proteolytic activation of myxoviruses in infected chick embryos by using aprotinin].
The mandatory step in reproduction of myxoviruses (influenza viruses and paramyxoviruses) is proteolytic shearing of viral glycoproteins activating the infectivity of virions. Such activation of myxoviruses is realized by trypsin-like proteases of the host. This study demonstrated that proteolytic activation of virions could be inhibited by a physiological inhibitor of proteases, aprotinine. A single injection of aprotinine (preparations Gordox or Contrycal) into chick embryos infected with various influenza viruses (WSN/34, Udorn/72) and paramyxoviruses (Sendai/960, NDV/La Sota, NDV/Queensland) blocked shearing of viral glycoproteins, HA, FO, HNO as a result of which noninfectious virions with unsheared glycoproteins were predominantly synthesized. In aprotinine-treated embryos, multicycle virus infection was markedly decreased which led to the 10(4)-fold or greater reduction of the virus yield. The antiviral effect of protease inhibitors and possibilities of their practical use in viral diseases are discussed.
Zhirnov
O P
OP
Ovcharenko
A V
AV
Bukrinskaia
A G
AG
D000818:Animals; D007611:Aprotinin / Q000494:pharmacology*; D002642:Chick Embryo; D004789:Enzyme Activation / Q000187:drug effects; D006023:Glycoproteins / Q000378:metabolism; D009975:Orthomyxoviridae / Q000187:drug effects* / Q000201:enzymology / Q000472:pathogenicity; D009976:Orthomyxoviridae Infections / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism; D014771:Virion / Q000187:drug effects / Q000201:enzymology / Q000472:pathogenicity; D014779:Virus Replication / Q000187:drug effects
D006023:Glycoproteins; D014764:Viral Proteins; D007611:Aprotinin; D010447:Peptide Hydrolases
Podavlenie proteoliticheskoi aktivastsii miksovirusov v zarazhennykh kurinykh 'embrionakh s pomoshch'iu aprotinina.