Micellar enzymology.
Experimental approaches to modelling the enzymatic function of biological membranes are discussed. Emphasis is given to pseudohomogeneous systems such as proteolipid complexes and enzymes in organic solvents; the latter are solubilized with phospholipids or synthetic surfactants. Methods for producing and studying such micellar systems are considered. The key research problems of micellar enzymology are formulated and its relation to enzyme membranology is discussed. Finally, the new potentialities are noted of applied enzymology (biotechnology) offered by application of a colloidal solution of water in organic solvents as a microheterogeneous medium for enzymatic reactions.
K
K
Martinek
A V
AV
Levashov
N
N
Klyachko
Y L
YL
Khmelnitski
I V
IV
Berezin
D002384:Catalysis; D003102:Colloids; D004143:Dioctyl Sulfosuccinic Acid; D004798:Enzymes / Q000378:metabolism*; D006838:Hydrocarbons; D007700:Kinetics; D008051:Lipid Bilayers; D008066:Lipolysis; D008566:Membranes / Q000201:enzymology; D008823:Micelles; D008956:Models, Chemical; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010743:Phospholipids; D012243:Rhodopsin; D012995:Solubility; D012997:Solvents; D013379:Substrate Specificity; D013501:Surface-Active Agents; D014867:Water
D003102:Colloids; D004798:Enzymes; D006838:Hydrocarbons; D008051:Lipid Bilayers; D008823:Micelles; D010743:Phospholipids; D012997:Solvents; D013501:Surface-Active Agents; D014867:Water; D004143:Dioctyl Sulfosuccinic Acid; D012243:Rhodopsin