Single cell analysis of calcium mobilization in anti-immunoglobulin-stimulated B lymphocytes.
A rapid increase in the concentration of intracellular free calcium ([Ca++i]) in B cells after mIg crosslinking has been documented previously by fluorimetric analysis of cell populations loaded with the fluorescent Ca++ indicator Quin 2. Although providing a valuable indication of Ca++ mobilization in the population as a whole, it has not been possible to determine whether only a subpopulation of the cells or the entire population exhibits this response. In this report, we describe the marriage of flow cytometry and Quin 2 technology, which permits discrimination of Ca++ mobilization by subpopulations of cells, as well as in whole populations. We have determined that the entire mIg+ population exhibits a synchronous increase in [Ca++i] rapidly after stimulation. Furthermore, all members of the population appear to undergo an approximately equal response.
Ransom
J T
JT
DiGiusto
D L
DL
Cambier
J C
JC
D000634:Aminoquinolines / Q000494:pharmacology; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000502:physiology*; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002453:Cell Cycle / Q000187:drug effects; D005434:Flow Cytometry / Q000379:methods; D007473:Ion Channels / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism; D012503:Saponins / Q000494:pharmacology
D000634:Aminoquinolines; D000888:Antibodies, Anti-Idiotypic; D007473:Ion Channels; D011947:Receptors, Antigen, B-Cell; D012503:Saponins; D002118:Calcium