Aneuploidy assay on diethylstilbestrol by means of in situ hybridization of radioactive and biotinylated DNA probes on interphase nuclei.
Aneuploidy tests by means of in situ hybridization with chromosome-specific DNA probes on interphase nuclei have been carried out on human lymphocytes treated with diethylstilbestrol (DES). A DNA probe specific for chromosome Y (Y97), either radioactive or biotinylated, was used for the assays. Autoradiography or FITC-conjugated antibiotin antibodies were employed to visualize the hybridization sites. A significant increase of hyperdiploid nuclei was obtained with both procedures and a dose-related effect was revealed using the biotinylated probe. The results obtained, while giving further support to the evidence that DES is able to induce aneuploidy in cultured human cells, also indicate that the sensitivity of the assay can be improved by using biotinylated probes coupled with fluorescent antibodies.
Dipartimento di Genetica e Microbiologia A. Buzzati-Traverso, Pavia, Italy.
A
A
De Sario
P
P
Vagnarelli
L
L
De Carli
D000328:Adult; D000782:Aneuploidy; D001345:Autoradiography; D001710:Biotin; D002478:Cells, Cultured; D015342:DNA Probes; D004054:Diethylstilbestrol / Q000633:toxicity*; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D006801:Humans; D007399:Interphase; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology; D009693:Nucleic Acid Hybridization; D014316:Tritium
D015342:DNA Probes; D009153:Mutagens; D014316:Tritium; D001710:Biotin; D004054:Diethylstilbestrol