A method for silver impregnation of mammary myoepithelia.
Histochemical methods for microscopic visualization of mammary myoepithelial cells all yielded considerable variation in completeness of myoepithelial cell staining. Although extremely variable, silver impregnation occasionally gave tissue sections containing myoepithelia having excellent microanatomical detail and contrast with other tissue elements. Consequently, sources of variation in the silver technique were considered. Composition of the tissue fixative and pH of the silver impregnating solution were most critical. A final method is presented which gives consistent, complete silver impregnation of myoepithelia, where both the cell body and cell processes are clearly evident. The staining procedure is not light sensitive, nor is acid cleaning of glassware necessary. Tissue sections from lactating mouse, rat, hamster and goat are presented; tissue from other species should stain as well. The procedure should greatly facilitate the study of the function of myoepithelial cells and the visualization of these cells in mammary pathology.
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EV
Caruolo
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P
Minchew
D000818:Animals; D006224:Cricetinae; D004847:Epithelial Cells; D005260:Female; D006652:Histological Techniques; D006863:Hydrogen-Ion Concentration; D008321:Mammary Glands, Animal / Q000166:cytology*; D051379:Mice; D051381:Rats; D012834:Silver; D013194:Staining and Labeling / Q000379:methods*
D012834:Silver