Virion proteins and the perspectives of gene manipulations in vaccine preparation.
The achievements and perspectives of genetic manipulations are described aiming at preparation of first generation subunit vaccines based on the synthesis in bacterial and eukaryotic cells of full-sized virion proteins expressing the main antigenic determinants. The preparation of such vaccines in bacterial cells seems out of perspective in the case of influenza, human hepatitis B, foot- and - mouth disease and some other viruses due to the peculiarities of structure and synthesis as well as low immunogenicity of the monomeric form of virion polypeptides. However, biotechnological procedures using eukaryotic cells and higher eukaryotic vectors and in part also yeast cells allowed to obtain full-sized virion proteins in a highly immunogenic state with good effects.
Tickhonenko
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D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D005537:Aphthovirus / Q000276:immunology; D004274:DNA, Recombinant; D000939:Epitopes; D005818:Genetic Engineering; D006023:Glycoproteins / Q000276:immunology; D006515:Hepatitis B virus / Q000276:immunology; D006801:Humans; D009980:Influenza A virus / Q000276:immunology; D046911:Macromolecular Substances; D011499:Protein Processing, Post-Translational; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013329:Structure-Activity Relationship; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*; D014771:Virion / Q000276:immunology
D000956:Antigens, Viral; D004274:DNA, Recombinant; D000939:Epitopes; D006023:Glycoproteins; D046911:Macromolecular Substances; D014764:Viral Proteins; D014765:Viral Vaccines