Stimulation of mitochondrial pyruvate transport in rat renal cortex by phenylephrine.
Phenylephrine effect on liver and kidney cortex mitochondrial pyruvate concentration was investigated. While in liver the alpha 1-adrenergic agent produced a decrease in pyruvate content, a significant increase was observed in kidney, even in the presence of 0.5 mM alpha-cyano-4-hydroxy-cinnamate. These changes were not observed when pyruvate was formed by intramitochondrial transamination of alanine, suggesting a role for the pyruvate transport across mitochondrial membranes in the regulation of mitochondrial pyruvate metabolism in kidney cortex. This was corroborated measuring the phenylephrine effect on pyruvate carboxylation.
Oliver
J
J
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Biológicas, Universidad de Granada, Spain.
Sola
M M
MM
Salto
R
R
Vargas
A M
AM
D000409:Alanine / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D003373:Coumaric Acids / Q000494:pharmacology; D005943:Gluconeogenesis / Q000187:drug effects; D007656:Ketoglutaric Acids / Q000494:pharmacology; D007672:Kidney Cortex / Q000187:drug effects* / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D010656:Phenylephrine / Q000494:pharmacology*; D011773:Pyruvates / Q000493:pharmacokinetics*; D019289:Pyruvic Acid; D051381:Rats; D011919:Rats, Inbred Strains
D003373:Coumaric Acids; D007656:Ketoglutaric Acids; D011773:Pyruvates; D010656:Phenylephrine; C018254:alpha-cyano-3-hydroxycinnamate; D019289:Pyruvic Acid; D000409:Alanine