Simple measurement of captopril in plasma by high-performance liquid chromatography with ultraviolet detection.
Captopril is an orally active inhibitor of angiotensin-converting enzyme. A rapid, accurate, and sensitive high-performance chromatography (HPLC) method is described for measuring plasma concentrations of captopril. Captopril was stabilized by forming an adduct with p-bromophenacyl bromide. This adduct was measured by HPLC using a C-18 reverse-phase column and monitoring the column effluent by ultraviolet absorption at 260 nm. The method proved to be linear in the clinical range of 10-1,000 ng/ml. The plasma levels of captopril in a young patient given a small oral dose were determined by this method.
Department of Pediatrics, Hospital for Sick Children, Toronto, Ontario, Canada.
J
J
Klein
P
P
Colin
E
E
Scherer
M
M
Levy
G
G
Koren
D000098:Acetophenones; D001726:Bisacodyl / Q000031:analogs & derivatives; D002216:Captopril / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006801:Humans; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
D000098:Acetophenones; D007202:Indicators and Reagents; D012997:Solvents; D001726:Bisacodyl; D002216:Captopril; C006821:4-bromophenacyl bromide; C001659:dihydroxydiphenyl-pyridyl methane