Quantitative amino acid analysis of individual snail neurons by open tubular liquid chromatography.
A method is described for the determination of amino acids in individual cells. The amino acids are derivatized with naphthalene-2,3-dicarboxaldehyde and then analyzed by open tubular liquid chromatography with amperometric detection. The total volume present after derivatization is approximately 25 nL. It was possible to quantitatively determine 17 amino acids in three different neurons of the land snail Helix aspersa. Quantitation was accomplished through the use of two internal standards and a calibration curve. Alanine was found to be the most abundant amino acid by about a factor of 2 over glutamine in all three types of neurons. This method has the advantages of sensitivity in the attomole range (5 X 10(-9) M) and selectivity for a specific class of compounds and is at least as reliable as other methods used for single-cell analysis.
Department of Chemistry, University of North Carolina, Chapel Hill 27599-3290.
M D
MD
Oates
B R
BR
Cooper
J W
JW
Jorgenson
D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D002853:Chromatography, Liquid; D006372:Helix, Snails / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*
D000596:Amino Acids