Complexes of polyoma virus medium T antigen and cellular proteins.
Antibodies against synthetic peptides corresponding to the carboxyl-terminal six amino acids, Lys-Arg-Ser-Arg-His-Phe (KF), and an internal region, Glu-Glu-Glu-Glu-Tyr-Met-Pro-Met-Glu (EE), of polyoma virus medium T antigen were used successively to purify medium T antigen by affinity chromatography. Medium T antigen from cell extracts was first bound to anti-KF antibodies and released from the immune complex with excess KF peptide; then it was bound to anti-EE antibodies and released with excess EE peptide. Two proteins, pp60c-src and a new protein of approximately equal to 61,000 Da (61-kDa protein), were copurified because they formed complexes with medium T antigen. The 61-kDa protein-medium T antigen complex was detected in extracts from wild-type-infected and transformed cells but not from cells infected with NG59 virus, which has a mutation in the medium T gene and is transformation defective. Instead, NG59 medium T antigen formed a complex with another cellular protein of approximately equal to 72,000 Da.
T
T
Grussenmeyer
K H
KH
Scheidtmann
M A
MA
Hutchinson
W
W
Eckhart
G
G
Walter
D000818:Animals; D000957:Antigens, Viral, Tumor; D007163:Immunosorbent Techniques; D046911:Macromolecular Substances; D051379:Mice; D008970:Molecular Weight; D011120:Polyomavirus / Q000276:immunology / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D016392:Proto-Oncogene Proteins pp60(c-src); D014764:Viral Proteins / Q000378:metabolism*
D000957:Antigens, Viral, Tumor; D046911:Macromolecular Substances; D011506:Proteins; D011518:Proto-Oncogene Proteins; D014764:Viral Proteins; D016392:Proto-Oncogene Proteins pp60(c-src)