Gene transfer into brain tumor cell lines: reporter gene expression using various cellular and viral promoters.
In the present study we determined the optimal conditions for transferring DNA into rat and human brain tumor cell lines of glial and neuronal origin using electroporation as the transfection method. Gene transfer efficiency was measured in terms of transient chloramphenicol acetyltransferase (CAT) activity and stable neomycin expression. Moreover, the activity of a variety of cellular and viral promoters in brain tumor cell lines of distinct origin was characterized. The results revealed various expression patterns, including glial as well as neuronal specific promoter activity.
Oellig
C
C
Ludwig Institute for Cancer Research, Stockholm, Sweden.
Seliger
B
B
D000818:Animals; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004560:Electricity; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral / Q000235:genetics; D006801:Humans; D011401:Promoter Regions, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured
D015500:Chloramphenicol O-Acetyltransferase