Peptidergic and aminergic innervation of the facial nucleus of the rat with special reference to ontogenetic development.
The distribution and ontogenetic development of several neuropeptides such as enkephalin, substance P, somatostatin, neuropeptide Y, and of monoamines such as serotonin and catecholamines in the facial nucleus of the rat were investigated with immunocytochemistry. The neuropeptides were concentrated in certain subnuclei. Enkephalin-immunoreactive fibers were distributed in the medial and dorsal subnuclei, substance P in the intermediate and dorsal subnuclei, somatostatin in the intermediate subnucleus, and neuropeptide Y in the dorsal subnucleus. The amines were distributed evenly throughout the nucleus. These distribution patterns suggest that peptidergic fibers are closely related to the functions of different subnuclei, while fibers containing monoamines are more basic--not specific to individual muscles. Few of these fibers were observed in the prenatal stage of the rat, but they increased markedly in number during the first postnatal week, and had established their innervation pattern by the tenth postnatal day, which coincides with the establishment of nerve-muscle innervation. The present study further showed that fibers containing serotonin are supplied mainly from the raphe nucleus, that catecholamine fibers are from neurons containing catecholamine surrounding the facial nucleus, and that fibers containing neuropeptide Y are from the lateral part of the caudal medullary reticular formation. These findings suggest that catecholamine and neuropeptide Y are not both present in the single neurons projecting to the facial nucleus.
Senba
E
E
Tohyama
M
M
Shiotani
Y
Y
Kawasaki
Y
Y
Kubo
T
T
Matsunaga
T
T
Emson
P C
PC
Steinbusch
H W
HW
D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development; D001679:Biogenic Amines / Q000032:analysis*; D001923:Brain Chemistry; D002766:Cholecystokinin / Q000032:analysis; D004622:Embryo, Mammalian; D004745:Enkephalins / Q000032:analysis; D005154:Facial Nerve / Q000032:analysis / Q000254:growth & development* / Q000601:surgery; D005260:Female; D005455:Fluorescent Antibody Technique; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009478:Neuropeptide Y; D009496:Neurotensin / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000032:analysis; D013004:Somatostatin / Q000032:analysis; D013373:Substance P / Q000032:analysis; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
D001679:Biogenic Amines; D004745:Enkephalins; D009419:Nerve Tissue Proteins; D009478:Neuropeptide Y; D012701:Serotonin; D013373:Substance P; D009496:Neurotensin; D013004:Somatostatin; D002766:Cholecystokinin; D014446:Tyrosine 3-Monooxygenase