Antibacterial activity of seminalplasmin, a basic protein from bovine seminal plasma.
Seminalplasmin, a 6,000 dalton antimicrobial protein present in bovine seminal plasma, is shown to inhibit growth and/or RNA synthesis in several bacterial species. In only one strain out of twenty one belonging to fourteen species, did both RNA synthesis and growth appear to be resistant to seminalplasmin. The antibacterial activity of seminalplasmin, in the case of E. coli, was also studied as a function of its concentration and of time; the minimal concentration of the protein required for 100% bactericidal activity was only about twice that required for 100% bacteriostatic activity. The killing of E. coli cells proceeded in two phases, a slow phase and then a rapid one, and required several hours for completion. Several bacterial species tested secreted proteases into the medium that destroyed seminalplasmin.
K H
KH
Scheit
M
M
Zimmer
N S
NS
Rao
K S
KS
Prasad
P M
PM
Bhargava
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects; D001419:Bacteria / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D002417:Cattle; D004755:Enterobacteriaceae / Q000187:drug effects; D013293:Enterococcus faecalis / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D007700:Kinetics; D008297:Male; D010447:Peptide Hydrolases / Q000378:metabolism; D011506:Proteins / Q000378:metabolism / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012329:RNA, Bacterial / Q000096:biosynthesis; D012661:Semen; D029607:Seminal Vesicle Secretory Proteins; D013211:Staphylococcus aureus / Q000187:drug effects
D000900:Anti-Bacterial Agents; D011506:Proteins; D012329:RNA, Bacterial; D029607:Seminal Vesicle Secretory Proteins; D010447:Peptide Hydrolases