Do the same hypothalamic neurons project to both amygdala and cerebellum?
The possibility that individual hypothalamic cells project to the cerebellum (as hypothalamo-cerebellar fibers) and amygdala (hypothalamo-amygdaloid fibers) by means of collaterals, was studied by means of retrograde transport of Fast blue (FB) and Rhodamine (R)-labeled latex microspheres. Our findings show that R-labeled latex microspheres are well-suited for double labeling studies in combination with other retrograde fluorescent tracers. Neurons retrogradely labeled from cerebellum or from amygdala were found in the same hypothalamic nuclei, and they were often located adjacent to each other. Some additional animals were injected with a third tracer, Nuclear yellow (NY), in the spinal cord. The spinal projecting neurons were located in the same regions as cells retrogradely labeled with FB and R. All projections are bilateral with an ipsilateral preponderance. Some neurons were double-labeled, and all combinations of double-labeling (FB/R, NY/R, FB/NY) were found. This shows that some hypothalamic neurons may project to both cerebellum and amygdala, some to the spinal cord and amygdala, and others to cerebellum and the spinal cord. No triple labeled neurons were observed.
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Dietrichs
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Haines
D000578:Amidines; D000679:Amygdala / Q000166:cytology*; D000818:Animals; D001562:Benzimidazoles; D002415:Cats; D002525:Cerebellar Cortex / Q000166:cytology*; D007031:Hypothalamus / Q000166:cytology*; D008863:Microspheres; D009434:Neural Pathways / Q000166:cytology; D012235:Rhodamines; D013116:Spinal Cord / Q000166:cytology; D013194:Staining and Labeling / Q000379:methods; D000096926:Benzenesulfonamides
D000578:Amidines; D001562:Benzimidazoles; D012235:Rhodamines; C031455:diamidino compound 253-50; C036574:nuclear yellow; D000096926:Benzenesulfonamides