Demonstration of separate phosphotyrosyl- and phosphoseryl- histone phosphatase activities in the plasma membranes of a human astrocytoma.
A plasma membrane preparation from a human astrocytoma contained p-nitrophenyl phosphate (pNPP), phosphotyrosyl histone, and phosphoseryl histone hydrolysis activities. The pNPPase and phosphotyrosyl histone phosphatase activities were inhibited by vanadate, whereas the phosphoseryl histone phosphatase activity was not; the latter activity was inhibited by pyrophosphate and nucleoside di- and triphosphates. When the membranes were solubilized by Triton X-100 and the solubilized proteins were subjected to column chromatography on DEAE-Sephadex, Sepharose 6B-C1, and wheat germ agglutinin-Sepharose 4B columns, the pNPPase activity from the phosphoseryl histone phosphatase activity. The results from column chromatography also indicated that there may be multiple phosphotyrosyl and phosphoseryl protein phosphatases in the plasma membranes.
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D009597:4-Nitrophenylphosphatase / Q000378:metabolism*; D001254:Astrocytoma / Q000201:enzymology*; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D011756:Diphosphates / Q000494:pharmacology; D006657:Histones / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D066298:In Vitro Techniques; D009596:Nitrophenols / Q000378:metabolism; D009711:Nucleotides / Q000494:pharmacology; D009943:Organophosphorus Compounds / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D010768:Phosphoserine / Q000378:metabolism; D019000:Phosphotyrosine; D014018:Tissue Distribution; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism; D014638:Vanadates; D014639:Vanadium / Q000494:pharmacology
D011756:Diphosphates; D006657:Histones; D009596:Nitrophenols; D009711:Nucleotides; D009943:Organophosphorus Compounds; D014639:Vanadium; D010768:Phosphoserine; D019000:Phosphotyrosine; C008644:nitrophenylphosphate; D014638:Vanadates; D014443:Tyrosine; D010749:Phosphoprotein Phosphatases; D010744:Phosphoric Monoester Hydrolases; C022394:phosphoserine phosphatase; D009597:4-Nitrophenylphosphatase