[Synthesis and immunochemical study of the antigenic determinant of the H-2Db molecule of the murine major histocompatibility complex].
Primary structure of murine class I histocompatibility antigens has been analysed to select possible antigenic determinant. Hexapeptide Leu-Gln-Gln-Leu-Ser-Gly, homologous to the region 95-100 of the H-2Db antigen heavy chain, was synthesised by stepwise elongation of peptide chain beginning from the COOH-terminal Gly. Rabbit anti-hexapeptide antibodies were obtained and shown to interact specifically with purified H-2Db antigen as well as with the native antigen on cell surface. These antibodies bind to lymphocytes of H-2b haplotype (C57BL/6 mice) but not H-2d (BALB/c) or H-2k (CBA). These data suggest that the region 95-100 is responsible for serologic differences between the alleles of H-2 antigens, i.e. it may be a xenotypic as well as an allotypic antigenic determinant. The latter was confirmed by study of interaction of the hexapeptide with allogeneic monoclonal antibodies specific to H-2Db antigen.
V V
VV
Iurovskiĭ
N G
NG
Safonova
S V
SV
Khaĭdukov
R G
RG
Vasilov
V V
VV
Demin
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D055598:Chemical Phenomena; D002621:Chemistry; D000939:Epitopes / Q000032:analysis*; D006183:H-2 Antigens / Q000032:analysis* / Q000276:immunology; D007124:Immunoenzyme Techniques; D051379:Mice; D008815:Mice, Inbred Strains; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000276:immunology; D011817:Rabbits
D000906:Antibodies; D000939:Epitopes; D006183:H-2 Antigens; D009842:Oligopeptides
Sintez i immunokhimicheskoe izuchenie antigennoĭ determinanty H-2Db-molekuly glavnogo kompleksa gistosovmestimosti myshi.