A novel H-2s class I molecule expressed on a B-cell leukemia from SJL/J mice.
Anti-H-2.33 [(B10.D2 X A)F1 anti-B10.A(5R)], which predominantly contains antibodies recognizing H-2Kb and IAb molecules, was found to be cytotoxic against DMLM 1678, a B-cell leukemia of SJL/J (H-2s) origin. The antiserum precipitated a typical class I (H-2-like) molecule from labeled tumor cell preparations as judged by molecular mass, papain susceptibility and association with beta 2-microglobulin. Sequential immunoprecipitation studies revealed that it was distinct from either H-2Ks or H-2Ds, the 2 molecules expressing the private antigens of the H-2s haplotype. Absorption analysis using congenic mice mapped the gene controlling the expression of the novel molecule telomeric to the S-region within the major histocompatibility complex.
Siwarski
D F
DF
Prat
M
M
Rogers
M J
MJ
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D005802:Genes, MHC Class II; D006183:H-2 Antigens / Q000032:analysis*; D007106:Immune Sera / Q000276:immunology; D007942:Leukemia, Experimental / Q000139:chemically induced / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D001613:beta 2-Microglobulin / Q000378:metabolism
D000951:Antigens, Neoplasm; D000939:Epitopes; D006183:H-2 Antigens; D007106:Immune Sera; D001613:beta 2-Microglobulin; D015127:9,10-Dimethyl-1,2-benzanthracene