Hapten-specific unresponsiveness in mice. III. Study of the B-cell functions of tolerant mice.
The features of B-cell tolerance induced in mice with the chemically reactive hapten 2,4,6-trinitrobenzenesulphonic acid were investigated after various antigenic challenges. A complete abolition of the IgG response was observed after challenge with trinitrophenol (TNP)-coupled haemocyanin (TNP-KLH) associated or not with Escherichia coli lipopolysaccharide. Carrier immunization with KLH (3 micrograms) followed by challenge with TNP-KLH led to a normal IgG anti-TNP response. However, tolerant mice given 3 micrograms TNP-KLH (instead of KLH) and a repeat injection of TNP-KLH exhibited a depressed IgG anti-TNP response. These results are discussed within the framework of tolerance induction in adult mature B cells.
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D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology*; D002352:Carrier Proteins; D000939:Epitopes; D006241:Haptens / Q000276:immunology*; D006433:Hemocyanins; D007108:Immune Tolerance; D007114:Immunization; D007116:Immunization, Passive; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011828:Radiation Chimera; D013154:Spleen / Q000166:cytology; D014302:Trinitrobenzenesulfonic Acid / Q000276:immunology
D002352:Carrier Proteins; D000939:Epitopes; D006241:Haptens; D007074:Immunoglobulin G; D014302:Trinitrobenzenesulfonic Acid; D006433:Hemocyanins