Effects of methotrexate on transfected DNA stability in mammalian cells.
The chromosomal locations, amounts, and level of expression of transfected, amplified c-myc and dihydrofolate reductase sequences were measured in cells cultured in the presence and absence of methotrexate. These studies show that the location and amount of transfected sequences, as well as the level of expression, were more variable when the cells were cultured in methotrexate.
Biomedical Sciences Division, Lawrence Livermore National Laboratory, Livermore, California 94550.
M G
MG
Pallavicini
P S
PS
DeTeresa
C
C
Rosette
J W
JW
Gray
F M
FM
Wurm
D000818:Animals; D015342:DNA Probes; D004274:DNA, Recombinant / Q000235:genetics; D005784:Gene Amplification / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D008727:Methotrexate / Q000494:pharmacology*; D051379:Mice; D009693:Nucleic Acid Hybridization; D011518:Proto-Oncogene Proteins / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics; D014162:Transfection
D015342:DNA Probes; D004274:DNA, Recombinant; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate