Cyclic AMP and c-myc gene expression in PY815 mouse mastocytoma cells.
The possibility was examined that inhibition of growth of PY815 mouse mastocytoma cells by N6,O2'-dibutyryladenosine 3',5'-cyclic monophosphate (DB cyclic AMP) results from inhibition of c-myc gene expression. Temporary increases in c-myc RNA which occurred soon after DB cyclic AMP treatment and upon removal of the drug were not consistent with direct inhibition of c-myc gene expression by DB cyclic AMP. The increases in c-myc RNA coincided with the passage through, or accumulation of cells in late G1-early S phase. It is proposed that cyclic AMP may stimulate c-myc gene expression which normally occurs only in late G1-early S phase in PY815 cells and that cyclic AMP prevents c-myc expression in cells at other phases of the cell cycle by inhibiting their progression past a cyclic AMP-sensitive restriction point in early G1 phase.
Le Gros
J
J
De Feyter
R
R
Ralph
R K
RK
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D000242:Cyclic AMP / Q000502:physiology*; D004274:DNA, Recombinant; D005786:Gene Expression Regulation / Q000187:drug effects; D007399:Interphase; D012515:Mast-Cell Sarcoma / Q000235:genetics*; D051379:Mice; D009857:Oncogenes; D012313:RNA / Q000378:metabolism; D013806:Theophylline / Q000494:pharmacology
D004274:DNA, Recombinant; D012313:RNA; D003994:Bucladesine; D013806:Theophylline; D000242:Cyclic AMP