Determination of collagen band positions on polyacrylamide gel without staining.
Protein band positions on a slab polyacrylamide gel were determined without staining, by blotting the bands onto a nitrocellulose membrane which was then stained to visualize the electrophoretic pattern. The unfixed, unstained bands were cut from the gel according to the copy pattern placed underneath the slab gel. Collagen alpha 1 and alpha 2 chains were separately extracted from the gel using this procedure.
Y
Y
Takehana
T
T
Hayashi
H
H
Konomi
M
M
Arima
D000596:Amino Acids / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D003094:Collagen / Q000032:analysis*; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D013194:Staining and Labeling
D000596:Amino Acids; D003101:Collodion; D003094:Collagen