In vitro radiation responses of human intracranial meningiomas & their modifications by 2-deoxy-D-glucose.
Radiation responses of 16 human intracranial meningiomas [hemangiopericyte, 4; fibrous, 4; transitional, 4; vascular, 3; and meningiothelial, 1] and their modifications by 2-deoxy-D-glucose (2-DG) have been studied in vitro using organ cultures derived from post-operative tumour specimens. Treatment induced cytogenetic damage viz., micronuclei formation and DNA content dispersion, were analysed as indices of the radiation damage. Results showed that the 60Co gamma ray induced micronuclei frequencies in meningiomas varied over a large range (0.7-6.2%). Presence of 2-DG (at equimolar concentration with glucose) for 4 h after irradiation, increased the radiation damage by a factor of up to 2.8 in nearly 70 per cent of the cases, although a decrease was observed in 20 per cent cases. These observations can be explained on the basis of the energy linked modulations of the competitive processes of repair and fixation of radiation induced DNA lesions. The results of the study suggest that the therapy of meningiomas could be improved by combining radiotherapy with administration of 2-DG.
Dwarakanath
B S
BS
Department of Biophysics, National Institute of Mental Health & Neuro Sciences, Bangalore.
Jain
V
V
D001932:Brain Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000532:radiotherapy*; D003131:Combined Modality Therapy; D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects; D003837:Deoxy Sugars / Q000627:therapeutic use*; D003847:Deoxyglucose / Q000627:therapeutic use*; D006801:Humans; D066298:In Vitro Techniques; D008579:Meningioma / Q000188:drug therapy / Q000378:metabolism / Q000532:radiotherapy*; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects / Q000528:radiation effects
D003837:Deoxy Sugars; D003847:Deoxyglucose