Inhibitory effects of 9-beta-D-xylofuranosyladenine 5'-triphosphate on DNA-dependent RNA polymerase I and II from cherry salmon (Oncorhynchus masou).
In order to determine the mode of action of cytostatic 9-beta-D-xylofuranosyladenine (xylo-A), the inhibitory effects of 9-beta-D-xylofuranosyladenine 5'-triphosphate (xylo-ATP) on DNA-dependent RNA polymerases I and II purified from cherry salmon (Oncorhynchus masou) liver nuclei were studied. This nucleotide showed strong inhibitory action on both RNA polymerases I and II. The K1 values are 14 microM for polymerase I and 5 microM for polymerase II (Km values of ATP are 37 microM for polymerase I and 40 microM for polymerase II). The mode of xylo-ATP was competitive with respect to the incorporation of AMP into RNA and non-competitive to UTP and CTP.
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Nakayama
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Saneyoshi
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D003838:Deoxyadenine Nucleotides / Q000494:pharmacology; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D012313:RNA / Q000096:biosynthesis; D012318:RNA Polymerase I / Q000037:antagonists & inhibitors*; D012319:RNA Polymerase II / Q000037:antagonists & inhibitors*; D012265:Ribonucleotides / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
D003838:Deoxyadenine Nucleotides; D012265:Ribonucleotides; C011746:3'-deoxyadenosine 5'-triphosphate; D012313:RNA; C046449:9-beta-xylofuranosyladenine 5'-triphosphate; D000255:Adenosine Triphosphate; D012319:RNA Polymerase II; D012318:RNA Polymerase I