Ciliary muscle choline acetyltransferase and acetylcholinesterase after ciliary ganglionectomy.
Choline acetyltransferase (ChAT; EC 2.3.1.6) and acetylcholinesterase (AChE; EC 3.1.1.7) activities were measured in cynomolgus monkey ciliary muscle 1 month and 6 or more months after ciliary ganglionectomy (CG) or post-ganglionic ciliary neurectomy (PCN). ChAT activity was undetectable and AChE activity was elevated 1 month after CG or PCN, while both averaged about 30% of normal levels 6 or more months after denervation. Four out of six eyes reinnervated by functional criteria 6-12 months after CG or PCN. In one of the two remaining eyes permanently denervated, ChAT was absent from the ciliary muscle. In the other, ChAT activity was about 50% of normal, similar to the reinnervated eyes, but the regenerated cholinergic nerves were not approximated to the ciliary muscle fibers.
Howe Laboratory of Ophthalmology, Harvard Medical School, Boston, MA.
K A
KA
Erickson-Lamy
C D
CD
Johnson
B
B
True-Gabelt
P L
PL
Kaufman
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D002924:Ciliary Body / Q000201:enzymology* / Q000294:innervation; D005260:Female; D005726:Ganglia, Parasympathetic / Q000502:physiology; D015171:Ganglionectomy; D008252:Macaca fascicularis
D002795:Choline O-Acetyltransferase; D000110:Acetylcholinesterase