Superior antiproliferative effects mediated by interferon-alpha entrapped in liposomes against a newly established human lung cancer cell line.
The purpose of this study was to characterize the antiproliferative activity of a recombinant interferon-alpha (IFN-alpha) against a newly established human adenocarcinoma cell line (DMS-4C) and to determine whether IFN-alpha entrapped in multilamellar liposomes had superior antitumor effects compared with free IFN-alpha. Treatment of DMS-4C cells with 100 U/ml of free IFN-alpha resulted in 34% cytostasis. IFN-alpha encapsulated in phosphatidylcholine/phosphatidylserine multilamellar vesicles produced growth inhibition of 67%, which was significantly greater than that produced by free IFN-alpha or by control liposomes containing only medium combined with free IFN-alpha. Moreover, kinetic analysis revealed that to produce significant cytolysis, free IFN-alpha had to be incubated with target cells for at least 24 h, whereas IFN-alpha encapsulated into liposomes required only 30 min of exposure.
Shin
D M
DM
Department of Medical Oncology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Fidler
I J
IJ
Bucana
C D
CD
Fan
D
D
Hong
W K
WK
Killion
J J
JJ
D000230:Adenocarcinoma / Q000188:drug therapy*; D000970:Antineoplastic Agents; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology / Q000627:therapeutic use*; D007700:Kinetics; D008081:Liposomes / Q000627:therapeutic use; D008175:Lung Neoplasms / Q000188:drug therapy*; D008875:Middle Aged; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D007370:Interferon Type I; D008081:Liposomes