Haplotype-specific suppression of antibody responses in vitro. III. Haplotype-specific suppressor factor (TsF-H) binds to but fails to suppress responses by the I-A mutant strain B6.C-H-2bm12.
Spleen cells from C57BL/6 and B6.C-H-2bm12 mice, both responder strains to GAT, differ in their ability to be suppressed by the monoclonal I-A-restricted, nonantigen-specific, but haplotype-specific suppressor factor, TsF-H, from the hybridoma 266A4.5. Whereas GAT-specific responses by C57BL/6 spleen cells are susceptible to TsF-H-mediated suppression, responses by bm12 spleen cells are nonsuppressible under the same conditions. Responses of both C57BL/6 and bm12 spleen cells are suppressed by monoclonal GAT-specific suppressor factors. The inability of TsF-H to suppress responses by the bm12 spleen cells presumably reflects the effects of the mutation in the beta-chain of the I-A antigen in this strain on the required I-A restriction between TsF-H and target cell for manifestation of suppressive activity. The data are discussed in terms of involvement of I-A or recognition of I-A in mediating suppression.
C M
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Sorensen
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Pierce
D000042:Absorption; D000818:Animals; D000917:Antibody Formation; D003433:Crosses, Genetic; D000939:Epitopes / Q000235:genetics* / Q000276:immunology; D005838:Genotype; D006183:H-2 Antigens / Q000235:genetics*; D006238:Haploidy; D006462:Hemolytic Plaque Technique; D006649:Histocompatibility Antigens / Q000235:genetics; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D007113:Immunity, Innate; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D013045:Species Specificity; D013154:Spleen / Q000166:cytology; D013491:Suppressor Factors, Immunologic / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D013997:Time Factors
D000939:Epitopes; D006183:H-2 Antigens; D006649:Histocompatibility Antigens; D000949:Histocompatibility Antigens Class II; C024138:I-E-antigen; D013491:Suppressor Factors, Immunologic