Reversible inhibition of normal human prokeratinocyte proliferation by type beta transforming growth factor-growth inhibitor in serum-free medium.
Type beta transforming growth factor-growth inhibitor (TGF beta/GI) causes normal human prokeratinocytes to arrest growth predominantly in the G1 phase of the cell cycle within 48 h after log phase cultures are exposed to the factor in serum-free medium. The growth arrest induced by TGF beta/GI is reversible because the cells from treated cultures can be replated into fresh medium and grown into large colonies. Normal prokeratinocytes are demonstrated to secrete TGF beta/GI-like molecules into the culture medium and to have specific cell surface receptors for this molecule. In contrast, a human squamous cell carcinoma, SCC-25, does not arrest growth when exposed to TGF beta/GI. These cells, unlike the normal prokeratinocytes, do not exhibit detectable cell surface receptors for the factor.
Shipley
G D
GD
Pittelkow
M R
MR
Wille
J J
JJ
Scott
R E
RE
Moses
H L
HL
D001769:Blood; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media; D000078404:Epidermal Cells; D004817:Epidermis / Q000187:drug effects; D006131:Growth Inhibitors / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D007399:Interphase / Q000187:drug effects; D007633:Keratins; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology*; D015291:Transforming Growth Factors
D003470:Culture Media; D006131:Growth Inhibitors; D010455:Peptides; D007633:Keratins; D015291:Transforming Growth Factors