A new approach for studying semithin sections of human pathological material: intermicroscopic correlation between light microscopy and scanning electron microscopy.
In order to obtain useful and complete information on the study of pathological material, we observed by scanning electron microscopy (SEM) the same semithin sections observed by light microscopy (LM). For this purpose, the specimen must have, at the same time, chromatic and electron dense characteristics. We thus developed different specimen preparation methods, subjecting the semithin sections to specific polychromatic staining with high atomic number (Z) elements, to monochromatic staining followed by routine contrasting with uranyl acetate and lead citrate, and to specific cytochemical and immunocytochemical procedures. The specimens were examined in sequence by LM, by SEM equipped with secondary electron, backscattered electron, transmitted electron detectors and by scanning transmission electron microscopy (S(T)EM).
Pasquinelli
G
G
Scala
C
C
Borsetti
G P
GP
Martegani
F
F
Laschi
R
R
D000199:Actins / Q000032:analysis; D001853:Bone Marrow / Q000648:ultrastructure; D006046:Gold; D006801:Humans; D007158:Immunologic Techniques; D007421:Intestine, Small / Q000648:ultrastructure; D007668:Kidney / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation / Q000379:methods*; D008867:Microtomy / Q000379:methods*; D010338:Pathology, Clinical / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods; D013270:Stomach / Q000648:ultrastructure
D000199:Actins; D006046:Gold