Quantitative detection of orthophosphate in polyacrylamide gels.
A one-step procedure for the detection of Pi-producing enzymes in polyacrylamide and agarose gels was developed using PPi hydrolysis by inorganic pyrophosphatase as a model reaction. The color reagent consists of acid molybdate, methyl green, and Triton X-305 and produces sharp greenish-blue bands in places of Pi accumulation. The color is stable and its intensity is linearily related to enzyme amounts in the gel.
A
A
Baykov
S
S
Volk
D000180:Acrylic Resins; D043564:Inorganic Pyrophosphatase; D008739:Methyl Green; D008982:Molybdenum; D017830:Octoxynol; D010710:Phosphates / Q000032:analysis*; D011092:Polyethylene Glycols; D011755:Pyrophosphatases; D013053:Spectrophotometry; D013194:Staining and Labeling
D000180:Acrylic Resins; D010710:Phosphates; C016680:polyacrylamide gels; C044659:molybdate; D011092:Polyethylene Glycols; D008982:Molybdenum; D008739:Methyl Green; D017830:Octoxynol; D011755:Pyrophosphatases; D043564:Inorganic Pyrophosphatase