Effect of cyclosporine A on procoagulant activity in mononuclear blood cells and monocytes in vitro.
Monocytes and macrophages synthesize thromboplastin when induced by a number of compounds. Our experiments show that cyclosporine A (CyA) enhances the synthesis of procoagulant activity in monocytes and in whole mononuclear cells (MNC) about two-fold when phytohaemagglutinin (PHA) is the stimulant. The enhancement is inhibited by actinomycin D and cycloheximide. CyA alone had no effect, but in the presence of PHA or phorbol ester CyA induces or enhances the synthesis of a vitamin K-dependent procoagulant in the MNC cultures. This procoagulant acts synergistically with thromboplastin synthesized in the same cultures.
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E
Carlsen
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AC
Mallet
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H
Prydz
D001798:Blood Proteins / Q000096:biosynthesis; D002478:Cells, Cultured; D003524:Cyclosporins / Q000494:pharmacology*; D006801:Humans; D007962:Leukocytes / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D010835:Phytohemagglutinins / Q000494:pharmacology; D011516:Prothrombin / Q000096:biosynthesis*; D012313:RNA / Q000096:biosynthesis; D013268:Stimulation, Chemical; D013997:Time Factors; D014859:Warfarin / Q000494:pharmacology
D001798:Blood Proteins; D003524:Cyclosporins; D010835:Phytohemagglutinins; D014859:Warfarin; D012313:RNA; D011516:Prothrombin