Quantitative differences in host cell reactivation of ultraviolet-damaged virus in human skin fibroblasts and epidermal keratinocytes cultured from the same foreskin biopsy.
Repair efficiency of cultured cells may be estimated by measuring the ability of a particular cell type to support virus damaged by an appropriate agent. In this study we have compared the inactivation of ultraviolet (254 nm)-damaged herpes simplex virus in human fibroblast and epidermal keratinocyte cell lines derived from the same foreskin biopsy and found the epithelial cells to be a factor of 3 times less efficient in supporting the damaged virus. The two different cell types show comparable ultraviolet inactivation of clone-forming ability, indicating that the difference is specific to viral host cell reactivation. This study required the development of a quantitative infectious centers assay for the measurement of viral titer in human epithelial cells, a system which may be of more general application in studies of potential human carcinogens.
Tyrrell
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Pidoux
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D000328:Adult; D001706:Biopsy; D002478:Cells, Cultured; D004260:DNA Repair; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000382:microbiology; D006801:Humans; D007633:Keratins; D008297:Male; D008875:Middle Aged; D018139:Simplexvirus / Q000254:growth & development / Q000528:radiation effects*; D012867:Skin / Q000378:metabolism / Q000382:microbiology* / Q000528:radiation effects; D014466:Ultraviolet Rays; D014775:Virus Activation
D007633:Keratins