[In vitro translation of RNA from lizard epididymis and identification of poly A RNA coding for a major androgen-dependent protein].
During the reproductive period (spring) the lizard epididymis secretes a soluble protein of an apparent molecular weight (MW) of 19,000, the protein L. This androgen dependent protein disappears during post-nuptial atrophy of the epididymis (summer). At two time intervals (spring and summer) total RNA were extracted and poly (A) RNA were prepared. The RNA were translated in a cell-free system (rabbit reticulocyte lysate) in the presence of 35S methionine. Labelled translation products were analyzed by polyacrylamide gel electrophoresis under denaturing conditions. Electrophoresis were preceded or not by immunoprecipitation with an antiserum raised against protein L. RNA extracted during spring coded for several unique bands including five immunoprecipitated proteins with close-related MW (21,000 to 25,000). When RNA were translated in the presence of dog microsomes, the five previously detected protein bands disappear although a 19,000 MW immunoprecipitated protein was clearly demonstrated. These proteins were not detected when RNA extracted in summer were used. The protein L appears to be synthesized as preprotein(s). Its (unique or several?) messenger is of poly A type; it is present in spring and absent or undetectable in these experimental conditions in summer.
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Courty
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Morel
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Dufaure
D000728:Androgens / Q000235:genetics* / Q000302:isolation & purification; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004822:Epididymis / Q000378:metabolism*; D008116:Lizards; D008297:Male; D008970:Molecular Weight; D011061:Poly A / Q000235:genetics*; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics* / Q000302:isolation & purification; D012313:RNA / Q000235:genetics* / Q000302:isolation & purification; D012333:RNA, Messenger; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism
D000728:Androgens; D011506:Proteins; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA
Traduction in vitro d'ARN d'épididyme de lézard et identification d'un ARN poly A codant pour une protéine majeure androgéno-dépendante.