Intestinal transmission of macromolecules (BSA and FITC-dextran) in the neonatal pig: enhancing effect of colostrum, proteins and proteinase inhibitors.
The effects of colostrum and constituents/factors in colostrum which may influence intestinal macromolecular transmission in the newborn preclosure pig were investigated. Unsuckled piglets were given, by use of a stomach tube, bovine serum albumin (BSA) and fluorescein-isothiocyanate (FITC)-labelled dextran 70,000 (FITC-D) as markers together with colostrum or the factors under study. The serum levels of BSA and FITC-D 4 h after feeding were then determined as a measure of the transfer. It was found that the two colostrums tested, bovine and especially porcine, markedly enhanced the transmission of both BSA and FITC-D. Furthermore, increasing amounts of the model proteins, BSA and bovine IgG (50-200 mg/ml), significantly increased the transfer of FITC-D, whereas unlabelled dextran 70,000 given in similar amounts did not. Proteinase inhibitors obtained from sow colostrum or soy bean also enhanced the transmission of both BSA and FITC-D while the inactive inhibitors, given as trypsin-inhibitor complexes, had no effect. On the other hand, addition of a proteinase, porcine trypsin, significantly decreased the transmission of FITC-D. These findings indicate that the intestinal transmission of macromolecules in the preclosure piglet is governed by the amount of protein available in the intestine. Therefore, feeding colostrum with a high protein content and proteinase inhibitors is likely to favour efficient intestinal transmission, although other colostrum factors may also be of importance.
Weström
B R
BR
Ohlsson
B G
BG
Svendsen
J
J
Tagesson
C
C
Karlsson
B W
BW
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D002417:Cattle; D003126:Colostrum / Q000502:physiology*; D003911:Dextrans / Q000378:metabolism* / Q000494:pharmacology; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives*; D005452:Fluoresceins / Q000378:metabolism*; D007074:Immunoglobulin G / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000378:metabolism*; D046911:Macromolecular Substances / Q000378:metabolism; D011480:Protease Inhibitors; D011506:Proteins / Q000494:pharmacology*; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013552:Swine; D014357:Trypsin / Q000494:pharmacology; D014358:Trypsin Inhibitor, Bowman-Birk Soybean / Q000494:pharmacology
D003911:Dextrans; D005452:Fluoresceins; D007074:Immunoglobulin G; D046911:Macromolecular Substances; D011480:Protease Inhibitors; D011506:Proteins; D014358:Trypsin Inhibitor, Bowman-Birk Soybean; C015219:fluorescein isothiocyanate dextran; D012710:Serum Albumin, Bovine; D014357:Trypsin; D016650:Fluorescein-5-isothiocyanate