[The effect of benzene on the activity of the hepatic mono-oxygenase system of female rats as a function of age].
The aim of the work was an attempt to estimate the changes taking place in the hepatic microsomes of a rat, in the early phase of newborn life, maturation and growing old after peritoneal benzene administration. The observations were led on a smooth and rough endoplasmic reticulum fractions. The object of experiment were P-450-dependent monooxygenases cytochrome. In the experiment the female Wistar rats at age: 5-, 10-, 15-, 30-, 60 days old and 18 months old were used. 6 hours before decapitation the rats were given benzene in peritoneal way, in dose 250 mg per kg of the body weight. In a smooth and rough microsomes were indicated: protein contents, cytochromes P-450 and b5 contents and also the activity of NADPH-cytochrome c and NADH-cytochrome b5 reductases, aniline hydroxylase and 4-aminopyrine demethylase. Benzene revealed a changing influence on protein contents. In the rough fraction the negative influence was observed, but in the smooth fraction a clear stimulation was observed in certain age intervals. The influence of the discussed hydrocarbon on the cytochrome P-450 contents was similar in both subfractions. In animals entering puberty benzene was found to reduce P-450 contents. The activity of NADPH-cytochrome c reductase, 4-aminopyrine demethylase, and aniline hydroxylase was modified to different degrees depending on age of the animals, the inhibitory effect being prevailing. The contents of cytochrome b5 in SER and RER didn't change in extreme age groups but it was remarkably reduced during puberty. A significantly changing influence of benzene on the activity of NADH-cytochrome b5 reductase was observed both in smooth and rough fractions.
Z II Katedry i Zakładu Histologii i Embriologii Sl. AM w Katowicach-Ligocie.
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D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D001554:Benzene / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000187:drug effects; D005260:Female; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D009245:NADH Dehydrogenase / Q000378:metabolism; D010105:Oxygenases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D003577:Cytochrome P-450 Enzyme System; D010105:Oxygenases; D009245:NADH Dehydrogenase; D001554:Benzene
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