An alkaline-phosphatase staining method in avidin-biotin immunohistochemistry.
An avidin-biotin alkaline-phosphatase (ABAP) staining method has been developed for the labeling of tissue sections and cell smears. The introduction of alkaline phosphatase as a marker enzyme through an avidin bridge results in excellent immunocytochemical labeling of different antigens using poly- and monoclonal antibodies. This technique avoids problems with endogenous peroxidase activity that sometimes occur using peroxidase staining procedures. The introduction of a preformed avidin-biotin alkaline-phosphatase complex (ABAPC) makes the presented technique as simple to handle as the widely used avidin biotin-peroxidase complex method (ABC). The ABAPC technique could be combined with other enzymatic labelings for double immunoenzymatic staining.
Ormanns
W
W
Schäffer
R
R
D000469:Alkaline Phosphatase; D001360:Avidin; D001710:Biotin; D005069:Evaluation Studies as Topic; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D007120:Immunochemistry / Q000379:methods*; D010047:Ovalbumin / Q000031:analogs & derivatives*; D013194:Staining and Labeling / Q000379:methods* / Q000592:standards
D001360:Avidin; D001710:Biotin; D010047:Ovalbumin; D000469:Alkaline Phosphatase