Olfactory-specific cytochrome P-450 (P-450olf1; IIG1). Gene structure and developmental regulation.
The olfactory neuroepithelium is the principal site of interaction for airborne molecules, mainly odorants, in the organism. The presence of an active cytochrome P-450-dependent oxidative metabolism in this tissue has not yet been studied as well as the hepatic cytochrome P-450-dependent oxidations. In this report, we describe cytochrome P-450olf1 (IIG1), a P-450 gene expressed at high levels uniquely in the olfactory epithelium. By Southern analysis and genomic DNA cloning, we demonstrate that a single copy of the P-450olf1 gene is present in the rat genome and contains 9 exons. We conclude that rat P-450IIG1 is a single gene subfamily. P-450olf1 gene expression was activated after birth in both male and female Sprague-Dawley rats and remained active in adult olfactory epithelium. A first maximum level of expression was reached around postnatal day 21. The coincidence between the temporal gene activation of P-450olf1 and the postnatal increase in the sensitivity of olfactory response to odorants is consistent with a potential role of this enzyme in olfactory function.
Department of Pharmacology, Biocenter of the University of Basel, Switzerland.
P
P
Nef
T M
TM
Larabee
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K
Kagimoto
U A
UA
Meyer
D000375:Aging; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D015342:DNA Probes; D004848:Epithelium / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005091:Exons; D005260:Female; D005786:Gene Expression Regulation; D005796:Genes; D015698:Genomic Library; D008099:Liver / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D005810:Multigene Family; D009831:Olfactory Mucosa / Q000254:growth & development*; D051381:Rats; D011919:Rats, Inbred Strains; D015183:Restriction Mapping; D014158:Transcription, Genetic; D015533:Transcriptional Activation
D003062:Codon; D015342:DNA Probes; D003577:Cytochrome P-450 Enzyme System