Immunological study of Pseudomonas aeruginosa extracellular slime.
Crude extracellular slime (C-ES) was isolated from 15 laboratory strains of Pseudomonas aeruginosa. Glycolypoprotein (GLP) was prepared from partially purified C-ES by the phenol treatment method. Lipopolysaccharides (LPS) were isolated from P. aeruginosa and Salmonella typhi-murium. Some correlation has been found between the quantity of the produced C-ES and virulence of P. aeruginosa for mice. Toxicity (LD50 value) and the yield of GLP did not correlate with the strain virulence. C-ES and GLP contained hexose, protein and hexosamine. Heptoses (characteristic LPS components) were present in C-ES and practically absent from GLP. Paper chromatographic analysis of GLP showed the presence of uronic acids, galactose, glucose and ribose in all strains; GLP of most strains contained rhamnose and a low amount of mannose and several strains xylose. Uronic acids, galactose and, probably, ribose and mannose were characteristic ES components. C-ES and GLP were studied in active and passive mouse protection. ES isolated from P. aeruginosa strains of different O serogroups or immunotypes induced a marked cross protective response.
E S
ES
Stanislavsky
N A
NA
Yushkova
L S
LS
Edvabnaya
N M
NM
Landsman
I G
IG
Zaidner
D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins; D003429:Cross Reactions; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology*; D006384:Hemagglutination; D007114:Immunization; D007116:Immunization, Passive; D008074:Lipoproteins / Q000032:analysis / Q000276:immunology*; D019081:O Antigens; D011552:Pseudomonas Infections / Q000276:immunology; D011550:Pseudomonas aeruginosa / Q000276:immunology* / Q000472:pathogenicity; D012486:Salmonella typhimurium / Q000276:immunology; D014574:Uronic Acids / Q000032:analysis; D014774:Virulence
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D006023:Glycoproteins; D008074:Lipoproteins; D019081:O Antigens; D014574:Uronic Acids; C025431:extracellular slime proteins, bacterial