Identification of phosphotyrosine in yeast proteins and of a protein tyrosine kinase associated with the plasma membrane.
[32P]Phosphotyrosine was detected in a hydrolysate of yeast proteins after in vivo labeling with [32P]phosphoric acid. The phosphoamino acid was present in cells exponentially growing on glucose as well as in cells that had reached the stationary phase of growth. Also, a plasma membrane preparation was shown to phosphorylate casein on tyrosine residues.
Castellanos
R M
RM
Mazón
M J
MJ
D002364:Caseins / Q000378:metabolism; D002462:Cell Membrane / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008565:Membrane Proteins / Q000032:analysis; D019000:Phosphotyrosine; D011494:Protein Kinases / Q000032:analysis*; D011505:Protein-Tyrosine Kinases; D012441:Saccharomyces cerevisiae / Q000032:analysis* / Q000254:growth & development; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis
D002364:Caseins; D005656:Fungal Proteins; D008565:Membrane Proteins; D019000:Phosphotyrosine; D014443:Tyrosine; D011494:Protein Kinases; D011505:Protein-Tyrosine Kinases