[Effect of caffeine on the Ca2+-transport function of sarcoplasmic reticulum vesicles in the rat myocardium].
The effects of caffeine on active transport of Ca2 by heavy and light fractions of rat myocardial microsomes were investigated with the use of a Ca2+-selective electrode and nephelometry. It was found that under the effect of caffeine (5 mM) the rate of Ca2 transport in the presence of oxalate decreased by 30 to 40%. The caffeine-induced inhibition was prevented by ruthenium and tetracaine, thus suggesting the inhibitor specificity. Since caffeine is a specific blocker of Ca2 transport to the terminal cisterns of the skeletal muscle sarcoplasmic reticulum, it is assumed that the microsomal fraction of rat myocardium contains terminal cistern fragments.
D S
DS
Benevolenskiĭ
E V
EV
Men'shikova
D O
DO
Levitskiĭ
V B
VB
Ritov
Iu P
IuP
Kozlov
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002110:Caffeine / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D066298:In Vitro Techniques; D008861:Microsomes / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D012430:Ruthenium Red / Q000494:pharmacology; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*; D013748:Tetracaine / Q000494:pharmacology
D013748:Tetracaine; D012430:Ruthenium Red; D002110:Caffeine; D002118:Calcium
Vliianie kofeina na Ca2+-transportiruiushchuiu funktsiiu puzyr'kov sarkoplazmaticheskogo retikuluma iz miokarda krysy.