A wasp venom mastoparan-induced polyphosphoinositide breakdown in rat peritoneal mast cells.
The phospholipid metabolism of rat peritoneal mast cells stimulated with mastoparan, a secretagogue purified from wasp venom, was investigated. Mastoparan at 20 micrograms/ml caused a rapid secretion of histamine. Mastoparan induced a transient decrease of phosphatidylinositol 4,5-biphosphate on 32P labeling and generation of a water-soluble degradation product, inositol trisphosphate on [3H]inositol labeling, suggesting the activation of phospholipase C upon stimulation.
Y
Y
Okano
H
H
Takagi
T
T
Tohmatsu
S
S
Nakashima
Y
Y
Kuroda
K
K
Saito
Y
Y
Nozawa
D000818:Animals; D001514:Bee Venoms / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects; D066298:In Vitro Techniques; D007295:Inositol Phosphates / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D010455:Peptides; D010529:Peritoneal Cavity; D010716:Phosphatidylinositols / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014862:Wasp Venoms / Q000494:pharmacology*
D001514:Bee Venoms; D007295:Inositol Phosphates; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D010716:Phosphatidylinositols; D014862:Wasp Venoms; C037500:mastoparan