Modified methods for preparation of cryostat sections of skeletal muscle.
We developed two modified methods for the preparation of cryostat sections of skeletal muscle: one using solid carbon dioxide and the other using the ultra-low freezer to prepare 2-methylbutane (isopentane) for freezing of skeletal muscle. The results were quite satisfactory, with good preservation of cellular details and histochemical properties. Freezing artifacts were minimal when compared with the standard liquid nitrogen method.
Wu
J S
JS
Hogan
G R
GR
Morris
J D
JD
D000251:Adenosine Triphosphatases / Q000032:analysis; D000328:Adult; D000818:Animals; D005615:Freezing; D006652:Histological Techniques; D006801:Humans; D009132:Muscles / Q000166:cytology* / Q000201:enzymology / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling; D014021:Tissue Preservation
D000251:Adenosine Triphosphatases