Cloning and characterization of the beta-amylase gene from Bacillus polymyxa.
The gene for beta-amylase was isolated from Bacillus polymyxa by molecular cloning in B. subtilis. B. subtilis cells containing this gene express and secrete an amylase which resembles the B. polymyxa beta-amylase and barley beta-amylase in terms of the products it generates during carbohydrate hydrolysis. Starch hydrolysis with this beta-amylase produces maltose, not glucose, whereas maltotriose and cycloheptaose are resistant to the action of this beta-amylase. The enzyme has a molecular weight of approximately 68,000. Restriction endonuclease mapping demonstrated that the DNA inserted in pBD64 and containing the gene is approximately 3 kilobases in length.
Friedberg
F
F
Rhodes
C
C
D000681:Amylases / Q000235:genetics*; D001407:Bacillus / Q000201:enzymology / Q000235:genetics*; D001412:Bacillus subtilis / Q000235:genetics; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005796:Genes; D005798:Genes, Bacterial; D008320:Maltose / Q000378:metabolism; D008970:Molecular Weight; D013213:Starch / Q000378:metabolism; D001614:beta-Amylase / Q000235:genetics* / Q000378:metabolism
D008320:Maltose; D013213:Starch; D004262:DNA Restriction Enzymes; D000681:Amylases; D001614:beta-Amylase