An attempt to measure glomerular plasma flow by the cationic dyes alcian blue and ruthenium red.
Autoradiography showed that 3H-labelled alcian blue injected into the rat renal artery was concentrated in the glomeruli. To test if the uptake could be used as a measure of local glomerular plasma flow, the one-pass extraction was measured in 19 rats. The average extraction was 31.9% (SD 8.0%). Impurities and heterogeneity of the tracer as well as reversible binding to erythrocytes may have contributed to the low estimated extraction. The average extraction of 103Ru-ruthenium red was 26.3%. We conclude that neither of these dyes are suitable for measurement of glomerular plasma flow.
O J
OJ
Lavik
K
K
Aukland
D000423:Alcian Blue; D000818:Animals; D005260:Female; D007211:Indoles; D007677:Kidney Function Tests / Q000379:methods; D007678:Kidney Glomerulus / Q000098:blood supply*; D008297:Male; D051381:Rats; D012039:Regional Blood Flow; D012428:Ruthenium; D012430:Ruthenium Red
D007211:Indoles; D012430:Ruthenium Red; D012428:Ruthenium; D000423:Alcian Blue