Separation of Pneumocystis carinii from the lung of the steroid-suppressed rat.
Pneumocystis carinii pneumonia was induced in rats by chronic corticosteroid immunosuppression. The parasite was separated from virtually all contaminating host cells using the technique of unit gravity sedimentation. Cellular contamination was routinely below 0.02%. The same technique allowed partial separation of the cyst from the trophozoite stage.
Department of Medical Microbiology, St. Mary's Hospital Medical School, London, U.K.
M B
MB
Taylor
C S
CS
Easmon
D000305:Adrenal Cortex Hormones; D000818:Animals; D002499:Centrifugation, Density Gradient; D005260:Female; D007108:Immune Tolerance; D008168:Lung / Q000382:microbiology*; D011010:Pneumocystis / Q000302:isolation & purification*; D011020:Pneumonia, Pneumocystis / Q000382:microbiology*; D051381:Rats; D011919:Rats, Inbred Strains
D000305:Adrenal Cortex Hormones