Preparation of pancreatin and purification of lipase from hog pancreas.
Pancreatin containing high activities of proteolytic enzymes, amylase and lipase was prepared from optimally autolyzed hog pancreas. About one hundred grams of pancreatin were obtained from one kilogram of hog pancreas. Lipase was purified from the pancreatin preparation through steps of mild alkaline solution extraction, removing proteolytic enzymes by affinity adsorption, first ammonium sulfate fractionation, Sephacryl S-200 gel filtration, and secondary ammonium sulfate fractionation. By these steps, the purity of the enzyme increased 14 fold and the recovery of the enzyme activity was 33%. The purified lipase was not homogeneous and contained several contaminating proteins when examined by disc polyacrylamide gel electrophoresis.
Chang
C T
CT
Cheng
S W
SW
Sung
H Y
HY
Su
J C
JC
D000681:Amylases / Q000302:isolation & purification; D000818:Animals; D004386:Duodenum / Q000201:enzymology; D008049:Lipase / Q000302:isolation & purification*; D010179:Pancreas / Q000201:enzymology*; D010194:Pancreatin / Q000302:isolation & purification*; D013552:Swine
D010194:Pancreatin; D008049:Lipase; D000681:Amylases