Enzyme immunoassay for elcatonin, a synthetic analogue of eel calcitonin, in plasma.
An enzyme immunoassay for the direct measurement of elcatonin, a synthetic analogue of eel calcitonin with hypocalcemic activity, in rat and dog plasma was developed. Elcatonin was conjugated to beta-galactosidase using an N-succinimidyl ester, and the product was used as the tracer. The antibody-bound tracer was precipitated using a second antibody. The nonequilibrated procedure gave better sensitivity and precision than the equilibrated procedure. The nonequilibrated method was sensitive at quantifiable concentrations as low as 0.2 ng/mL using 0.1 mL of plasma and was also reproducible, with RSD values generally less than +/- 10%. Plasma levels in rats and dogs were determined by this competitive enzyme immunoassay after intramuscular or intravenous injection of 40 U/kg (8 micrograms/kg) of elcatonin.
Aikawa
R
R
Ohyama
K
K
Mori
E
E
Hironaka
K
K
Miyake
M
M
Morishita
M
M
D000818:Animals; D002116:Calcitonin / Q000031:analogs & derivatives* / Q000097:blood*; D003432:Cross-Linking Reagents; D004524:Eels; D006041:Goats; D007124:Immunoenzyme Techniques; D007273:Injections, Intramuscular; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D001616:beta-Galactosidase / Q000302:isolation & purification; D005719:gamma-Globulins / Q000302:isolation & purification
D003432:Cross-Linking Reagents; D005719:gamma-Globulins; D002116:Calcitonin; D001616:beta-Galactosidase; C012755:elcatonin