The interferon-induced enzyme 2-5A synthetase adenylates tRNA.
The interferon-induced enzyme 2-5A synthetase is shown to adenylate tRNA. Yeast tRNAPhe was incubated with the enzyme in the presence of double stranded RNA (in this case polyI-polyC) and ATP or deoxyATP. The reaction products were analyzed by ribonuclease T1 digestion of the tRNA, polyacrylamide gel electrophoresis and autoradiography. Using ATP, the 2-5A synthetase adds one, two or three AMP residues to the 3'-end of the tRNA whereas when dATP is replacing ATP, only one nucleotide unit is added. It is concluded that one of the mechanisms of the interferon-induced antiviral effect may be an inhibition of the translation process caused by an inactivation of tRNA molecules by a 2-5A synthetase catalyzed 2'-adenylation of the 3'-end.
J
J
Justesen
H
H
Worm-Leonhard
D
D
Ferbus
H U
HU
Petersen
D015088:2',5'-Oligoadenylate Synthetase / Q000096:biosynthesis / Q000378:metabolism*; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D003838:Deoxyadenine Nucleotides / Q000378:metabolism; D007372:Interferons / Q000494:pharmacology*; D007668:Kidney / Q000201:enzymology; D011070:Poly I-C / Q000494:pharmacology; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism*; D006163:Ribonuclease T1 / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000032:analysis
D003838:Deoxyadenine Nucleotides; D012346:RNA, Transfer, Amino Acyl; D000249:Adenosine Monophosphate; D000255:Adenosine Triphosphate; D007372:Interferons; D015088:2',5'-Oligoadenylate Synthetase; D006163:Ribonuclease T1; C026600:2'-deoxyadenosine triphosphate; D011070:Poly I-C