Effect of calcium channel agonist Bay K 8644 on calcitonin secretion from a rat C-cell line.
Bay K 8644, a novel dihydropyridine, stimulates calcitonin secretion in a dose-dependent manner from a rat medullary thyroid carcinoma cell line, rMTC 6-23, and causes an increase in cytosolic free calcium concentration, as measured by quin-2. These effects are competitively inhibited by nifedipine, and completely abolished in the absence of extracellular calcium. These data suggest that calcium influx via voltage-dependent calcium channels plays a crucial role in the regulation of cytosolic free calcium concentration and calcitonin secretion.
R
R
Hishikawa
M
M
Fukase
M
M
Takenaka
T
T
Fujita
D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; D000634:Aminoquinolines; D000818:Animals; D002116:Calcitonin / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D003593:Cytoplasm / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D007473:Ion Channels / Q000187:drug effects*; D009543:Nifedipine / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D012636:Secretory Rate / Q000187:drug effects; D013961:Thyroid Gland / Q000378:metabolism*
D000634:Aminoquinolines; D007473:Ion Channels; D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; C035738:Quin2-acetoxymethyl ester; D002116:Calcitonin; D009543:Nifedipine; D002118:Calcium