Calcium-dependent stimulation of acetylcholine release by substance P and vasoactive intestinal polypeptide.
In the guinea-pig myenteric plexus-longitudinal muscle preparation from the small intestine, the release of acetylcholine evoked by substance P and vasoactive intestinal polypeptide was examined in vitro. Both neuropeptides stimulated efflux of [3H]acetylcholine from myenteric neurons in a calcium-dependent manner. This observation is consistent with the view that neurotransmitter release elicited by neuropeptides requires the presence of extracellular calcium ions.
W M
WM
Yau
J A
JA
Dorsett
M L
ML
Youther
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000502:physiology*; D004558:Electric Stimulation; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007421:Intestine, Small / Q000187:drug effects; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D009197:Myenteric Plexus / Q000187:drug effects; D013373:Substance P / Q000494:pharmacology*; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*; D014702:Veratrine / Q000494:pharmacology
D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D014702:Veratrine; D000109:Acetylcholine; D002118:Calcium