Analogue inhibitor of 2-5A action: effect on the interferon-mediated inhibition of encephalomyocarditis virus replication.
Chemically synthesised CH3Sp(A2'p)2A2'pp3'OCH3 has been used to assess the importance of the ppp(A2'p)nA (n greater than or equal to 2: 2-5A) system in the antiviral action of interferon against encephalomyocarditis virus (EMC). It inhibits activation of the 2-5A-dependent RNase by 2-5A in intact mouse L929 cells and cell-free systems. In interferon-treated, EMC-infected L929 cells it inhibits 2-5A-mediated rRNA cleavage and partially restores EMC RNA synthesis and virus yield. Activation of the 2-5A-dependent RNase must, therefore, play some part in interferon action against the growth of EMC virus in such cells.
Watling
D
D
Serafinowska
H T
HT
Reese
C B
CB
Kerr
I M
IM
D000227:Adenine Nucleotides / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D000818:Animals; D002474:Cell-Free System; D004686:Encephalomyocarditis virus / Q000254:growth & development*; D004789:Enzyme Activation / Q000187:drug effects; D007372:Interferons / Q000037:antagonists & inhibitors*; D007739:L Cells; D051379:Mice; D009841:Oligonucleotides / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D009843:Oligoribonucleotides / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D014176:Protein Biosynthesis; D012335:RNA, Ribosomal / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism; D013873:Thionucleotides / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*
D000227:Adenine Nucleotides; D009841:Oligonucleotides; D009843:Oligoribonucleotides; D012335:RNA, Ribosomal; D013873:Thionucleotides; C023505:2',5'-oligoadenylate; D007372:Interferons; D012260:Ribonucleases