Lack of correlation of methylation and alphafetoprotein and albumin gene expression during liver growth, in hepatocellular carcinomas, and during hepatocarcinogenesis.
Analysis of AFP and albumin genes fails to demonstrate a correlation of gene activity with the degree of gene methylation as determined by restriction endonuclease fragments obtained using the isoschizomer pair, Hpa II and Msp I. There is no difference in the methylation patterns of DNA from high and low producing tissues, such as fetal and adult liver for AFP, hepatoma 7777 and 9098 for AFP; adult lung, brain, kidney and liver for albumin; fetal liver and brain or heart for AFP, etc. Minor selective differences in gene methylation that correlate with AFP or albumin gene expression cannot be ruled out.
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S
Sell
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Longley
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Boulter
D000418:Albumins / Q000235:genetics*; D000818:Animals; D004247:DNA / Q000378:metabolism; D005786:Gene Expression Regulation; D008099:Liver / Q000254:growth & development; D008114:Liver Neoplasms, Experimental / Q000235:genetics*; D008297:Male; D008745:Methylation; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011912:Rats, Inbred ACI; D011916:Rats, Inbred F344; D011919:Rats, Inbred Strains; D000509:alpha-Fetoproteins / Q000235:genetics*
D000418:Albumins; D012333:RNA, Messenger; D000509:alpha-Fetoproteins; D004247:DNA