Further studies on the action of baclofen on neurons of the dorsolateral septal nucleus of the rat, in vitro.
Baclofen hyperpolarizes rat dorsolateral septal neurons in a concentration-dependent manner. The apparent dissociation constant for this action is 200 nM. The baclofen-induced hyperpolarization is due to an increase in potassium conductance with a reversal potential of -88 mV. Of the various potassium and calcium channel blockers we tested, only 4-aminopyridine blocked baclofen-induced hyperpolarizations. Our results suggest that baclofen does not activate a calcium-dependent potassium conductance.
Stevens
D R
DR
Gallagher
J P
JP
Shinnick-Gallagher
P
P
D015761:4-Aminopyridine; D000631:Aminopyridines / Q000494:pharmacology; D000818:Animals; D001418:Baclofen / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D004553:Electric Conductivity; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects; D011188:Potassium / Q000378:metabolism; D051381:Rats; D012686:Septal Nuclei / Q000187:drug effects*; D019789:Tetraethylammonium; D013757:Tetraethylammonium Compounds / Q000494:pharmacology
D000631:Aminopyridines; D007473:Ion Channels; D013757:Tetraethylammonium Compounds; D019789:Tetraethylammonium; D015761:4-Aminopyridine; D001418:Baclofen; D011188:Potassium