Acceleration of nucleic acid hybridization rate by polyethylene glycol.
The addition of polyethylene glycol to filter-bound nucleic acid hybridization greatly increases the hybridization rate. With single-stranded probes, the increase obtained with polyethylene glycol is significantly greater than that obtained with dextran sulfate. Additionally, polyethylene glycol is easier to manipulate and less expensive than dextran sulfate.
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Amasino
D004269:DNA, Bacterial; D016264:Dextran Sulfate; D003911:Dextrans; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization / Q000187:drug effects*; D010957:Plasmids; D011092:Polyethylene Glycols / Q000494:pharmacology*; D012313:RNA
D004269:DNA, Bacterial; D003911:Dextrans; D011092:Polyethylene Glycols; D012313:RNA; D016264:Dextran Sulfate