Detection of rubella antibody using an optical immunosensor.
Since the first biosensor was reported in 1962 different sensors have been developed in different areas; their attraction being that they hold out the possibility of a rapid test which can specifically detect the analyte, without the necessity for additional reagents. Immunosensors make use of antibodies and can be extremely specific if the appropriate monoclonal antibodies are used. We report on the development of an optical immunosensor used for the detection of rubella antibody in serum, plasma and whole blood. The assay time is less than 10 min and requires no accurate measurement of the sample or any additional reagents. The sensitivity is in the region of 30 IU/ml; with a correlation of 94% with conventional assays.
Serono Diagnostics, Woking, Surrey, U.K.
R P
RP
Parry
C
C
Love
G A
GA
Robinson
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology; D001667:Binding, Competitive; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D012409:Rubella / Q000175:diagnosis; D012412:Rubella virus / Q000254:growth & development / Q000276:immunology*; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral