Molecular cloning and nucleotide sequence of a cDNA clone coding for rat brain myelin proteolipid.
A cDNA library from rat brain was constructed in pBR322 and screened with a 14-mer mixed oligonucleotide probe based on residues 231-235 of bovine proteolipid (PLP). A positive clone was isolated: it contained a 1334-base-pair cDNA insert and was subjected to DNA sequence analysis. The cDNA encoded information for the 276 amino acids of rat PLP. Comparison with bovine PLP sequence showed a complete amino acid sequence homology except for 4 amino acid residues.
Dautigny
A
A
Alliel
P M
PM
d'Auriol
L
L
Pham Dinh
D
D
Nussbaum
J L
JL
Galibert
F
F
Jollès
P
P
D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004274:DNA, Recombinant; D009185:Myelin Proteins / Q000235:genetics*; D018991:Myelin Proteolipid Protein; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D051381:Rats
D004274:DNA, Recombinant; D009185:Myelin Proteins; D018991:Myelin Proteolipid Protein; D004247:DNA