Immuno gold staining (IGS) and immuno gold silver staining (IGSS) for the identification of the plant pathogenic bacterium Erwinia amylovora (Burrill) Winslow et al.
For the identification of the plant pathogenic bacterium Erwinia amylovora, the immuno gold staining (IGS) and immuno gold silver staining (IGSS) techniques are tested. The IGS and IGSS methods are at least as sensitive an indirect immunofluorescence and require less primary antiserum. Moreover they have the advantage that the preparations can be conserved permanently and unchanged. The preparation of the IGS can be observed with transmitted light or--with considerable better result--using epipolarization microscopy. The IGSS method deserves special attention because of its high contrast in normal brigth field microscopy with transmitted light.
Van Laere
O
O
De Wael
L
L
De Mey
J
J
D000818:Animals; D004885:Erwinia / Q000302:isolation & purification*; D006046:Gold / Q000276:immunology; D008859:Microscopy, Polarization; D011817:Rabbits; D012834:Silver; D013194:Staining and Labeling
D012834:Silver; D006046:Gold