[Effect of chronic exposure to nitrogen dioxide on biogenic amine levels in the brain of guinea pigs].
The experiment was carried out on male guinea pigs exposed to NO2 at a constant concentration of 0.1 ppm 24 h daily for 1, 2.5 and 6 months, or NO2 concentration was increased to 0.5 ppm twice daily, between 7:00 and 8:00 AM and 4:00 and 5:00 PM. After the exposure to NO2 was over the animals were killed by decapitation. The cranium was opened and the brain removed, and afterwards at 0 degrees C the left hemisphere was dissected into discrete areas which were then frozen on dry ice and weighed. The tissues were kept for 2-3 days at -20 degrees C, and afterwards the content of noradrenaline, 5-hydroxytryptamine and 5-hydroxyindole-acetic acid was estimated spectrofluorometrically in examined parts of the brain (thalamus--hypothalamus pons + medulla oblongata hippocampus, cortex frontalis). It was shown that the applied NO2 concentrations brought about slight and nonspecific alterations in the content of the investigated amines in guinea pigs' brain.
Felińska
W
W
Szkilnik
R
R
Stanosek
B
B
Kamiński
M
M
Ludyga
K
K
Brus
R
R
D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D001679:Biogenic Amines / Q000378:metabolism*; D001923:Brain Chemistry / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D008297:Male; D009585:Nitrogen Dioxide / Q000633:toxicity*; D009638:Norepinephrine / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D013997:Time Factors
D000393:Air Pollutants; D001679:Biogenic Amines; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D009585:Nitrogen Dioxide; D009638:Norepinephrine
Wpływ przewlekłej ekspozycji dwutlenku azotu na zawartość amin biogennych w mózgu świnek morskich.