Enhanced transcription in a pre-B-like cell line transformed with cloned antibody genes after antigen-specific stimulation.
The transducing vector, pSV2-neo, carrying the rearranged immunoglobulin (Ig) heavy (mu) and light (kappa) chain genes specific for the hapten 2,4,6-trinitrophenyl (TNP) was introduced into a pre-B cell line. The transformants expressed the TNP-specific IgM receptor on the surface. Furthermore, the addition of TNP-bovine serum albumin (hapten-carrier conjugate) to the culture media activated the expression of the transferred Ig genes and several endogenous genes such as v-abl and beta-tubulin. However, expression of the beta2-microglobulin gene was not affected. The results presented in this paper show that transfection of cloned Ig genes into B cells is a useful system for establishing monoclonal B cell lines expressing functional Ig receptor molecules with defined hapten specificity.
Ochi
A
A
Hozumi
N
N
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002460:Cell Line; D003001:Cloning, Molecular; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D005786:Gene Expression Regulation; D007075:Immunoglobulin M / Q000032:analysis / Q000235:genetics; D008213:Lymphocyte Activation; D051379:Mice; D009578:Nitrobenzenes / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell / Q000032:analysis / Q000235:genetics; D013234:Stem Cells / Q000276:immunology* / Q000378:metabolism; D014158:Transcription, Genetic; D014170:Transformation, Genetic; D014301:Trinitrobenzenes / Q000032:analysis / Q000276:immunology*
D000939:Epitopes; D007075:Immunoglobulin M; D009578:Nitrobenzenes; D011947:Receptors, Antigen, B-Cell; D014301:Trinitrobenzenes