Serodiagnosis of human paragonimiasis caused by Paragonimus heterotremus.
Enzyme-linked immunosorbent assay (ELISA) and indirect hemagglutination tests (IHA) were evaluated for serodiagnosis of human paragonimiasis caused by Paragonimus heterotremus using homologous adult worm extract as antigen. IgG-ELISA was the most sensitive, being positive in all paragonimiasis sera tested while IHA and IgA-ELISA gave 88% and 59% positive rates respectively. Cross reactivity in IgG-ELISA was detected with fascioliasis sera, producing overall assay specificity of 97%. It is suggested that IgG-ELISA is a reliable serodiagnostic test for human paragonimiasis caused by Paragonimus heterotremus.
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Thailand.
S
S
Pariyanonda
W
W
Maleewong
V
V
Pipitgool
C
C
Wongkham
N
N
Morakote
P
P
Intapan
B
B
Thirakharn
N
N
Boonphadung
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis; D002415:Cats; D004797:Enzyme-Linked Immunosorbent Assay; D006386:Hemagglutination Tests; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D010237:Paragonimiasis / Q000175:diagnosis*; D010238:Paragonimus / Q000302:isolation & purification
D000909:Antibodies, Helminth; D007070:Immunoglobulin A; D007074:Immunoglobulin G