Analysis of serum placental alkaline phosphatase activity in testicular cancer and cigarette smokers.
Serum PLAP/PLAP-like enzymes are elevated in testicular cancer patients and also in heavy smokers. Such elevations were measurable with anti-PLAP polyclonal and monoclonal antibodies, and an antigenic heterogeneity was demonstrated in the circulating enzymes. Thus polyclonal antibody revealed more of tumor PLAP-like antigen while a monoclonal antibody H7 detected PLAP of smokers better. PLAP of smokers and PLAP-like enzyme of cancer (seminoma) patients showed similar characteristics with respect to glycosylation pattern, charge and hydrophobicity. By these properties they were differentiated from PLAP of placenta.
Department of Virology, National Bacteriological Laboratory, Stockholm, Sweden.
K
K
Koshida
T
T
Stigbrand
E
E
Munck-Wikland
H
H
Hisazumi
B
B
Wahren
D000469:Alkaline Phosphatase / Q000097:blood*; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000097:blood*; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D004407:Dysgerminoma / Q000201:enzymology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D058851:GPI-Linked Proteins; D006801:Humans; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000097:blood*; D008297:Male; D012907:Smoking / Q000097:blood*; D013736:Testicular Neoplasms / Q000201:enzymology*
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D058851:GPI-Linked Proteins; D007527:Isoenzymes; D000469:Alkaline Phosphatase; C019839:alkaline phosphatase, placental