Sodium current in single myocardial mouse cells.
Morphologically intact single myocardial cells of the adult mouse show a length of 132 +/- 20 microns, a width of 21 +/- 5 microns, and a height of 10 +/- 4 microns (all mean +/- SD) and are brick-like in shape. A one suction pipette method is used for voltage clamp of those single cells. The determined time constant of capacitive current tau = 35 +/- 14 microseconds is very short. Series resistance rs, membrane resistance rm, and membrane capacity Cm are calculated to be 192 +/- 48 k omega, 6.1 +/- 1.1 M omega, and 186 +/- 92 pF (all mean +/- SD), respectively. Assuming the specific unit membrane capacitance of 1 microF/cm2, a total membrane area of 1.86 X 10(-4) cm2 is determined yielding a specific membrane resistance Rm of 1,134 omega cm2. Settling time of voltage clamp is 30 microseconds. TTX-block of sodium current is described by 1:1 binding with a KD value of 1.4 X 10(-6) M. Using a reduced extracellular sodium concentration the maximum Na current is between 25 and 40 nA at voltages between -40 and -30 mV. Currents of between +20 and +30 mV reverse in an outward direction. Inward currents are approximated by a m3h model. The time constant of activation decreases from 0.7 ms at -60 mV to 0.12 ms at +20 mV. The time constant of inactivation falls from 9.1 ms at -60 mV to 0.6 ms at + 20 mV.(ABSTRACT TRUNCATED AT 250 WORDS)
K
K
Benndorf
W
W
Boldt
B
B
Nilius
D000818:Animals; D004553:Electric Conductivity; D004594:Electrophysiology; D006706:Homeostasis; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D008815:Mice, Inbred Strains; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D010477:Perfusion; D012964:Sodium / Q000378:metabolism*; D013779:Tetrodotoxin / Q000494:pharmacology
D007473:Ion Channels; D013779:Tetrodotoxin; D012964:Sodium