Studies of the GTPase domain of archaebacterial ribosomes.
Ribosomes from the methanogens Methanococcus vannielii and Methanobacterium formicicum catalyse uncoupled hydrolysis of GTP in the presence of factor EF-2 from rat liver (but not factor EF-G from Escherichia coli). In this assay, and in poly(U)-dependent protein synthesis, they were sensitive to thiostrepton. In contrast, ribosomes from Sulfolobus solfataricus did not respond to factor EF-2 (or factor EF-G) but possessed endogenous GTPase activity, which was also sensitive to thiostrepton. Ribosomes from the methanogens did not support (p)ppGpp production, but did appear to possess the equivalent of protein L11, which in E. coli is normally required for guanosine polyphosphate synthesis. Protein L11 from E. coli bound well to 23S rRNA from all three archaebacteria (as did thiostrepton) and oligonucleotides protected by the protein were sequenced and compared with rRNA sequences from other sources.
A A
AA
Beauclerk
H
H
Hummel
D J
DJ
Holmes
A
A
Böck
E
E
Cundliffe
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001105:Archaea / Q000201:enzymology*; D001419:Bacteria / Q000201:enzymology*; D001430:Bacteriocins; D005227:Fatty Acids / Q000494:pharmacology; D020558:GTP Phosphohydrolases / Q000502:physiology*; D006868:Hydrolysis; D016712:Mupirocin; D010455:Peptides / Q000494:pharmacology; D010744:Phosphoric Monoester Hydrolases / Q000502:physiology*; D011485:Protein Binding; D012329:RNA, Bacterial / Q000096:biosynthesis; D012335:RNA, Ribosomal / Q000096:biosynthesis; D012269:Ribosomal Proteins / Q000096:biosynthesis / Q000378:metabolism; D012270:Ribosomes / Q000201:enzymology*; D013045:Species Specificity; D013883:Thiostrepton / Q000378:metabolism / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D005227:Fatty Acids; D010455:Peptides; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; C027624:ribosomal protein L11; C009779:micrococcin; D016712:Mupirocin; D010744:Phosphoric Monoester Hydrolases; D020558:GTP Phosphohydrolases; D013883:Thiostrepton