Embryo dissociation, cell isolation, and cell reassociation.
In resolving the role of cell recognition events in the process of morphogenesis it is necessary to focus on single events against a background of many complex interactions. This article presents a series of approaches that are designed to do just that. It should be noted that with simplification there is a danger of oversight. Nevertheless, in learning about mechanisms of cellular movement at gastrulation we have found that cell separation techniques, simplified adhesion assays, and predictable antibody activities are helpful for approaching the complex mechanisms of morphogenesis.
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McClay
D000818:Animals; D000911:Antibodies, Monoclonal; D001666:Binding Sites, Antibody; D002449:Cell Aggregation; D002450:Cell Communication; D002452:Cell Count; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D004475:Ectoderm / Q000166:cytology / Q000502:physiology; D004707:Endoderm / Q000166:cytology / Q000502:physiology; D005855:Germ Layers / Q000166:cytology / Q000502:physiology; D006818:Hyalin / Q000166:cytology; D008648:Mesoderm / Q000166:cytology / Q000502:physiology; D008846:Micromanipulation / Q000295:instrumentation / Q000379:methods*; D012617:Sea Urchins / Q000166:cytology / Q000196:embryology*
D000911:Antibodies, Monoclonal