Gossypol modulation of mouse heart and liver lactate dehydrogenase isoenzymes as a function of gender.
Acute treatment with gossypol produced both tissue specific and sex-dependent alterations of M and H subunits of mouse lactate dehydrogenase. Gossypol induced female mouse heart cytoplasma H subunit and inhibited both enzyme subunits in the male mouse. Gossypol induced mouse liver mitochondrial lactate dehydrogenase subunit M in both sexes 50 hrs after acute drug administration while was ineffective on the testicular enzyme. Induction of hepatic lactate dehydrogenase may enhance NADH:NAD ratio, resulting in alteration of cellular redox status and reduction of NAD utilization by other dehydrogenases. The gossypol-mediated sex-dependent changes of heart lactate dehydrogenase isoenzymes may be of value in the prediction of certain aspects of gossypol toxicity.
Messiha
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Texas Tech University Health Sciences Center, School of Medicine, Lubbock 79430.
D000818:Animals; D003593:Cytoplasm / Q000187:drug effects / Q000201:enzymology; D005260:Female; D006072:Gossypol / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D051379:Mice; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology*; D012737:Sex Factors; D013737:Testis / Q000187:drug effects / Q000201:enzymology
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D006072:Gossypol