Genetic analysis of mutants with a reduced Ca2+-dependent K+ current in Paramecium tetraurelia.
Two mutants of Paramecium tetraurelia with greatly reduced Ca2+-dependent K+ currents have been isolated and genetically analyzed. These mutants, designated pantophobiac, give much stronger behavioral responses to all stimuli than do wild-type cells. Under voltage clamp, the Ca2+-dependent K+ current is almost completely eliminated in these mutants, whereas the Ca2+ current is normal. The two mutants, pntA and pntB, are recessive and unlinked to each other. pntA is not allelic to several other ion-channel mutants of P. tetraurelia. The microinjection of a high-speed supernatant fraction of wild-type cytoplasm into either pantophobiac mutant caused a temporary restoration to the wild-type phenotype.
Hinrichsen
R D
RD
Amberger
E
E
Saimi
Y
Y
Burgess-Cassler
A
A
Kung
C
C
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D004553:Electric Conductivity; D007473:Ion Channels / Q000502:physiology*; D009154:Mutation; D010247:Paramecium / Q000235:genetics* / Q000502:physiology; D011188:Potassium / Q000378:metabolism*; D013045:Species Specificity
D007473:Ion Channels; D011188:Potassium; D002118:Calcium