A tissue-specific transcription enhancer from the Drosophila yolk protein 1 gene.
The yolk protein 1 gene (yp1) of Drosophila melanogaster is expressed only in the ovarian follicle cells and the fat bodies of adult females. We have previously shown that a different cis-acting DNA region is required for each of these tissue specificities. In this paper we use germ line transformation to localize and characterized one of these tissue-specific regulatory regions. We demonstrate that a 125 bp segment of DNA located 196 bp upstream of the yp1 cap site is sufficient to determine the sex-, stage-, and fat body-specific expression of the yp1 gene. We also find that this region can confer yp1-specific expression on a heterologous Drosophila promoter. This specificity is retained when the region is in different orientations and at different distances from the heterologous promoter. Thus a small regulatory region acts in vivo as a positive enhancer to determine the fat body expression pattern of yp1.
M J
MJ
Garabedian
B M
BM
Shepherd
P C
PC
Wensink
D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D002872:Chromosome Deletion; D004331:Drosophila melanogaster / Q000235:genetics*; D004527:Egg Proteins / Q000235:genetics*; D004530:Egg Yolk; D004742:Enhancer Elements, Genetic; D005260:Female; D005796:Genes; D005809:Genes, Regulator; D008297:Male; D009154:Mutation; D011401:Promoter Regions, Genetic; D012313:RNA / Q000235:genetics; D014158:Transcription, Genetic
D004527:Egg Proteins; D012313:RNA