The western immunoblot procedure for HIV antibodies and its interpretation.
The Western blot is frequently used to confirm human immunodeficiency virus type 1 (HIV-1) screening tests as it permits the identification of antibodies to individual component polypeptides of HIV-1. The procedure is described and different criteria for evaluation of HIV-1 Western blots are compared. The significance and management of indeterminate blot patterns are discussed.
Jerome H. Holland Laboratory, American Red Cross, Rockville, MD 20855.
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D015153:Blotting, Western / Q000379:methods; D005189:False Positive Reactions; D015483:HIV Antibodies / Q000032:analysis*; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans
D015483:HIV Antibodies