Immunoreactive somatostatin in the rat retina: light microscopic immunocytochemistry and chromatographic characterization.
The retinas of adult, male Long-Evans rats contain somatostatin-like immunoreactive material (SLI) as detected by radioimmunoassay. The SLI co-chromatographs with synthetic somatostatin-14 on both gel permeation chromatography and reversed phase high performance liquid chromatography; no somatostatin-28-like material or higher molecular weight forms have been detected. Immunocytochemical methods detect SLI in at least two cell populations. The more abundant stained cells are at the inner margin of the inner nuclear layer and give off processes which form a dense meshwork of fine, varicose fibers at the outer border of the inner plexiform layer, as well as processes which pass into other sublaminas of the inner plexiform layer. Varicose immunoreactive fibers run vertically or obliquely through the inner nuclear layer and bifurcate at its outer margin, giving rise to horizontally running fibers in the outer plexiform layer. These observations are consistent with rat retinal SLI being contained within amacrine cells, at least some of which are interplexiform cells. With cholchicine pretreatment, a more sparse population of stained cells is detected in the ganglion cell layer. These cells give rise to processes which enter the inner plexiform layer. It is not known if these are ganglion cells or displaced amacrine cells.
Sagar
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Marshall
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Landis
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D000818:Animals; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D007124:Immunoenzyme Techniques; D008297:Male; D010455:Peptides / Q000032:analysis*; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D012160:Retina / Q000032:analysis* / Q000166:cytology; D013194:Staining and Labeling
D010455:Peptides; C029993:somatostatin-like peptides