Demonstration of lipid A-binding proteins on murine lymphoma cells using R-mutant gram-negative bacteria as a detector.
Some R-mutant Escherichia coli and Salmonella heavily adhered to murine lymphoma cells of B cell and T cell lineages. This adhesion was primarily mediated by membrane-localized proteins on tumor cells, which bind the polymyxin B-reactive hydrophilic structure of lipis A on bacteria. SDS-PAGE analysis of tumor cell membranes showed that proteins or glycoproteins of MW = around 45Kd, 25-35Kd and around 15Kd preferentially bind lipid A. Various lymphoma cell lines binding the bacteria at different levels possessed lipid A-binding proteins of slightly different compositions. We conclude that lymphoma cells carry not a single but a group of lipid A-binding proteins in their membranes.
Nakashima
I
I
Department of Immunology, Nagoya University School of Medicine, Japan.
Kubota
T
T
Zhang
Y
Y
Yoshida
T
T
Inagi
R
R
Isobe
K
K
Nagase
F
F
Ohta
M
M
Kato
N
N
Yokochi
T
T
D000818:Animals; D001422:Bacterial Adhesion; D002352:Carrier Proteins / Q000032:analysis*; D002460:Cell Line; D002462:Cell Membrane / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000502:physiology; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000502:physiology*; D007711:Klebsiella pneumoniae / Q000502:physiology; D008050:Lipid A; D008223:Lymphoma / Q000382:microbiology*; D051379:Mice; D009154:Mutation; D012475:Salmonella / Q000502:physiology
D002352:Carrier Proteins; D008050:Lipid A