Isolation and characterization of histamine-releasing peptides from human parotid saliva.
Peptides responsible for releasing histamine were purified from human parotid saliva. The amino acid composition of the peptides showed a high proportion of histidine, lysine and arginine. Molecular weights of these peptides were between 3000 and 5000 as determined by SDS-acrylamide gel electrophoresis. These peptides induced histamine release from rat-isolated mast cells accompanied with degranulation in a dose-dependent manner over the concentration range 5-50 micrograms/ml.
K
K
Sugiyama
Y
Y
Suzuki
H
H
Furuta
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001120:Arginine / Q000032:analysis; D002852:Chromatography, Ion Exchange; D004305:Dose-Response Relationship, Drug; D006636:Histamine Release / Q000187:drug effects*; D006639:Histidine / Q000032:analysis; D006801:Humans; D008239:Lysine / Q000032:analysis; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D008970:Molecular Weight; D010455:Peptides / Q000302:isolation & purification* / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012463:Saliva / Q000032:analysis*
D000596:Amino Acids; D010455:Peptides; D006639:Histidine; D001120:Arginine; D008239:Lysine