Receptor for the cell binding site of discoidin I.
Discoidin I, a developmentally regulated lectin in Dictyostelium discoideum, has been implicated in cell-substratum adhesion and ordered cell migration during aggregation. This depends on the cell binding site of discoidin I, which is distinct from its carbohydrate binding site. We have isolated a receptor for the cell binding site by affinity chromatography. The receptor binds immobilized discoidin I in the presence of 0.3 M galactose and can be eluted with gly-arg-gly-asp-his-asp, a synthetic peptide the sequence of which is found in discoidin I, and which blocks cell migration into aggregates. The receptor is a developmentally regulated cell-surface glycoprotein of apparent Mr approximately 67,000. Univalent antibodies specific for this glycoprotein block aggregation.
H J
HJ
Gabius
W R
WR
Springer
S H
SH
Barondes
D000268:Adhesiveness; D000908:Antibodies, Fungal / Q000276:immunology; D000946:Antigens, Fungal / Q000276:immunology; D001665:Binding Sites; D002846:Chromatography, Affinity; D004023:Dictyostelium / Q000032:analysis* / Q000254:growth & development / Q000502:physiology; D000070818:Discoidin Domain Receptors; D000070821:Discoidins; D000939:Epitopes / Q000276:immunology; D005656:Fungal Proteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D007140:Immunoglobulin Fab Fragments; D007152:Immunologic Capping; D037102:Lectins; D008970:Molecular Weight; D009068:Movement; D015800:Protozoan Proteins; D020794:Receptor Protein-Tyrosine Kinases; D011975:Receptors, Mitogen / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D000070821:Discoidins; D000939:Epitopes; D005656:Fungal Proteins; D006023:Glycoproteins; D007140:Immunoglobulin Fab Fragments; D037102:Lectins; D015800:Protozoan Proteins; D011975:Receptors, Mitogen; D000070818:Discoidin Domain Receptors; D020794:Receptor Protein-Tyrosine Kinases