Peptide-specific antibodies identify the alpha 2 chain as the proteoglycan subunit of type IX collagen.
Type IX collagen is a recently characterized product of chondrocytes. The molecules of this collagen are heterotrimers of three genetically distinct polypeptide chains. One of the three chains contains chondroitin and/or dermatan sulfate glycosaminoglycan chains, giving the molecule a proteoglycan character. In fact, Type IX collagen has been identified with the proteoglycan Lt (PG-Lt), first isolated by Noro, A., Kimata, K., Oike, Y., Shinomura, T., Maeda, N., Yano, S., Takahashi, N., and Suzuki, S. (1983) J. Biol. Chem. 258, 9323-9331 from chick embryonic tibia and femur. Based on amino acid sequences predicted from the nucleotide sequences of cDNA and genomic clones specific for two of the chains of Type IX collagen, we have synthesized oligopeptides representing portions of the two chains. In addition, an oligopeptide has been made based on a partial amino acid sequence of the third chain. Antibodies against the synthetic peptides have been generated in rabbits, and the polyclonal sera have allowed identification of the three genetically distinct polypeptide subunits of Type IX collagen. In addition, labeling with [35S]sulfate and treatment with chondroitinase ABC demonstrates that glycosaminoglycan chains are present on the subunit that has been given the designation alpha 2(IX).
Konomi
H
H
Seyer
J M
JM
Ninomiya
Y
Y
Olsen
B R
BR
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D002356:Cartilage / Q000032:analysis; D002478:Cells, Cultured; D002642:Chick Embryo; D003094:Collagen / Q000032:analysis* / Q000276:immunology; D000939:Epitopes / Q000032:analysis*; D046911:Macromolecular Substances; D010455:Peptides / Q000032:analysis; D011509:Proteoglycans / Q000032:analysis* / Q000276:immunology
D000906:Antibodies; D000939:Epitopes; D046911:Macromolecular Substances; D010455:Peptides; D011509:Proteoglycans; D003094:Collagen