Ultrastructural localisation of oxytocin and neurophysin in the ovine corpus luteum.
Polyclonal rabbit antisera raised against oxytocin, bovine neurophysin I and vasopressin were used, together with an immunogold complex, to localise the peptides in ultrathin sections of ovine corpus luteum. The only organelle which consistently showed gold labelling was the secretory granule of the large luteal cell. In non-consecutive sections of the same large luteal cell all the granules showed a similar level of labelling after oxytocin or neurophysin I antisera: however no immunolabelling was detected for vasopressin. Oxytocin and neurophysin seem to be rapidly lost after secretion since exocytosed granule cores showed no labelling above background levels.
D T
DT
Theodosis
F B
FB
Wooding
E L
EL
Sheldrick
A P
AP
Flint
D000818:Animals; D003338:Corpus Luteum / Q000032:analysis* / Q000648:ultrastructure; D005260:Female; D006046:Gold; D008184:Luteal Cells / Q000032:analysis / Q000648:ultrastructure; D008854:Microscopy, Electron; D009481:Neurophysins / Q000032:analysis* / Q000276:immunology; D010121:Oxytocin / Q000032:analysis* / Q000276:immunology; D012756:Sheep; D013194:Staining and Labeling
D009481:Neurophysins; D010121:Oxytocin; D006046:Gold