Human leukocyte elastase inhibitors: designed variants of human pancreatic secretory trypsin inhibitor (hPSTI).
Variants of human secretory trypsin inhibitor were constructed with the aim of producing inhibitors specific for human leukocyte elastase. Models of the hPSTI/HLE and hPSTI/chymotrypsin complexes were generated by computer aided protein design and used to plan better HLE inhibitors. This resulted in the production of the strongest and most specific inhibitors of HLE known.
GBF-Gesellschaft für Biotechnologische Forschung mbH, Braunschweig.
J
J
Collins
M
M
Szardenings
F
F
Maywald
H
H
Blöcker
R
R
Frank
H J
HJ
Hecht
B
B
Vasel
D
D
Schomburg
E
E
Fink
H
H
Fritz
D000818:Animals; D001120:Arginine / Q000494:pharmacology; D002417:Cattle; D002918:Chymotrypsin / Q000037:antagonists & inhibitors; D003198:Computer Simulation; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D007962:Leukocytes / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors*; D014359:Trypsin Inhibitor, Kazal Pancreatic / Q000097:blood* / Q000235:genetics; D014361:Trypsin Inhibitors / Q000097:blood*
D014361:Trypsin Inhibitors; D014359:Trypsin Inhibitor, Kazal Pancreatic; D001120:Arginine; D002918:Chymotrypsin; D010196:Pancreatic Elastase