Simultaneous determination of tranilast and metabolites in plasma and urine using high-performance liquid chromatography.
A method has been developed for the simultaneous determination of Tranilast, N-(3',4'-dimethoxycinnamoyl)anthranilic acid (N-5'), and metabolites in plasma and urine from humans, dogs and rodents administered N-5'. Total N-5' and metabolite N-3 conjugates were determined in human urine. Detection limits in plasma were 0.2 micrograms/ml for metabolite N-3-S and N-5' and 0.1 micrograms/ml for metabolites N-3 and N-4. In urine, detection limits were 2 micrograms/ml for metabolite N-3-S and N-5' and 1 micrograms/ml for metabolites N-3 and N-4. Metabolite N-4 was not identified in any sample assayed.
D K
DK
Slobodzian
J Y
JY
Hsieh
W F
WF
Bayne
D000818:Animals; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006868:Hydrolysis; D051379:Mice; D011786:Quality Control; D051381:Rats; D013045:Species Specificity; D062367:ortho-Aminobenzoates / Q000032:analysis* / Q000097:blood / Q000652:urine
D062367:ortho-Aminobenzoates; C012293:tranilast