Stimulation of myeloid colony development elaborated by colony forming cells--fibroblasts from rat developing ossicles.
Subcutaneous implantation of demineralized bone matrix in rats results in endochondral bone formation and bone-marrow development. The cascade of events leading to this process occurs following the accumulation of mesenchymal cells with colony forming cell fibroblasts (CFC-F) potential in the implanted area, a process which commences already 72 h post implantation. It is demonstrated herein that CFC-F from various stages of ossicle development (days, 3, 7, 10, 14 or 18) stimulate hemopoiesis to the same extent as judged by the number of granuloid-macrophage-progenitors (CFU-GM), developed as hemopoietic colonies, and by the ratio of granuloid to macrophagic colonies. High concentrations of CFC-F, however, tend to diminish the stimulatory capacity. Prostaglandin E, CFU-GM, CFC-F, ossicle, growth factors, microenvironment, hemopoiesis, development.
Department of Histology and Cell Biology, Sackler Faculty of Medicine, Tel-Aviv University, Ramat-Aviv, Israel.
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Bleiberg
D000818:Animals; D001846:Bone Development; D001854:Bone Marrow Cells; D001857:Bone Matrix / Q000637:transplantation; D001842:Bone and Bones / Q000166:cytology*; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006098:Granulocytes / Q000166:cytology; D006133:Growth Substances / Q000378:metabolism; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology; D007213:Indomethacin / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology; D008297:Male; D051381:Rats; D011921:Rats, Inbred WKY; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D007213:Indomethacin