Studies on subtype composition in natural leukocyte interferon preparations.
Antisera raised against partially purified human leukocyte interferon in goat and horse were exhaustively adsorbed and purified. Affinity chromatography using these antibodies gave 10,000-fold purification in one step without losing interferon activity. Then enzyme immunoassay was established with these antibodies and utilized for subtype analysis. Crude and affinity purified leukocyte interferons showed identical profiles of antiviral activity and immunoreactivity when fractionated by means of reversed phase high performance liquid chromatography. These results suggested the possibility of providing mixture of subtypes of leukocyte interferon occurring in natural host response, with comparable purity of recombinant ones.
Biotechnology Research Laboratories, JCR Pharmaceuticals Co. Ltd., Kobe, Japan.
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H
Shirono
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Ito
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Koga
D000818:Animals; D000906:Antibodies / Q000276:immunology; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007124:Immunoenzyme Techniques; D007370:Interferon Type I; D007962:Leukocytes / Q000032:analysis* / Q000276:immunology
D000906:Antibodies; D007370:Interferon Type I