The formylmethanofuran:tetrahydromethanopterin formyltransferase from Methanobacterium thermoautotrophicum delta H. Nucleotide sequence and functional expression of the cloned gene.
The formylmethanofuran:tetrahydromethanopterin formyltransferase (FTR) from Methanobacterium thermoautotrophicum delta H was cloned and its sequence was determined. The clone was contained on a 4.8-kilobase BamHI fragment of M. thermoautotrophicum DNA ligated into pBR329. When this fragment was subcloned into the phagemid pTZ18R, a functional enzyme was synthesized under control of the lac promoter. Sequence analysis revealed the presence of a ribosome binding site and a possible terminator structure. The absence of an identifiable promoter lends credibility to the open reading frame which is present 5' to ftr. The ftr gene encodes an acidic protein with a calculated molecular weight of 31,401. The sequence of FTR does not appear to be homologous to any other sequenced proteins, including proteins which use pterin substrates.
Department of Microbiology, University of Illinois, Urbana 61801.
A A
AA
DiMarco
K A
KA
Sment
J
J
Konisky
R S
RS
Wolfe
D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D015245:Deoxyribonuclease BamHI; D004926:Escherichia coli / Q000235:genetics; D019605:Euryarchaeota / Q000201:enzymology* / Q000235:genetics; D058973:Folate Receptors, GPI-Anchored; D015870:Gene Expression; D019877:Hydroxymethyl and Formyl Transferases; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D010957:Plasmids; D011956:Receptors, Cell Surface; D015183:Restriction Mapping; D014166:Transferases / Q000235:genetics*
D002352:Carrier Proteins; D004269:DNA, Bacterial; D058973:Folate Receptors, GPI-Anchored; D015345:Oligonucleotide Probes; D011956:Receptors, Cell Surface; D014166:Transferases; D019877:Hydroxymethyl and Formyl Transferases; C050840:formylmethanofuran-tetrahydromethanopterin formyltransferase; D015245:Deoxyribonuclease BamHI