[Use of avidin for speedy clearance of biotinylated immunoglobulins from the blood flow].
The phenomenon of a hundredfold more rapid blood clearance of biotinylated immunoglobulins after post-injection of an equiponderate dose of avidin is described. The concentration of 125I-labeled biotinylated IgG in rat circulation slowly decreased to 20% of the initial level in 24 hours. Avidin injection at any moment of this period induced a 90-95% reduction of blood radioactivity in 15 minutes. Up to 70% of the radioactivity was recovered in the liver. The technique of enhanced blood clearance developed in rats was checked in dogs using biotinylated monoclonal anti-human fibrinogen antibodies, capable of concentrating in dog thrombus. The results obtained offer the possibility of thrombus/blood contrast increase in radioimmunoimaging.
V V
VV
Sinitsyn
A G
AG
Mamontova
E E
EE
Chekneva
A A
AA
Shnyra
S P
SP
Domogatskiĭ
D000818:Animals; D000911:Antibodies, Monoclonal; D001360:Avidin; D001710:Biotin; D004285:Dogs; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D007457:Iodine Radioisotopes; D008024:Ligands; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013927:Thrombosis / Q000175:diagnosis
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D008024:Ligands; D001360:Avidin; D001710:Biotin
Ispol'zovanie avidina dlia uskorennogo vyvedeniia biotinilirovannykh immunoglobulinov iz krovotoka.