Change of alpha-fetoprotein content during cell cycle of human hepatoma cells in vitro: flow cytometric analysis.
The relationship between alpha-fetoprotein (AFP) content and phases of the cell cycle was investigated by simultaneous flow cytometric analysis of cellular DNA and AFP, using an AFP producing human hepatoma cell line (HuH-7). AFP content was evaluated by measuring fluorescence intensity of FITC after staining the cells with an indirect immunofluorescent method, and DNA content by fluorescence intensity of propidium iodide. The amount of intracellular AFP was highly variable but flow cytometric analysis produced a distinct profile. AFP content increased linearly with progression of the cell cycle, except for a modest dip in the early S phase. This suggests that AFP production and secretion are closely linked to the cell cycle.
Sasaki
K
K
Murakami
T
T
Kawasaki
S
S
Okita
K
K
Takemoto
T
T
Takahashi
M
M
D006528:Carcinoma, Hepatocellular / Q000032:analysis* / Q000473:pathology; D002453:Cell Cycle; D002460:Cell Line; D004273:DNA, Neoplasm / Q000032:analysis; D005434:Flow Cytometry; D006801:Humans; D008113:Liver Neoplasms / Q000032:analysis* / Q000473:pathology; D000509:alpha-Fetoproteins / Q000032:analysis*
D004273:DNA, Neoplasm; D000509:alpha-Fetoproteins