Allergenicity and cross-reactivity of rye grass pollen extracts revealed by monoclonal antibodies.
Using monoclonal antibodies the immunogenic and allergenic characteristics of rye Group I were redefined by SDS-PAGE analysis and immunoblotting. The purified rye Group I from NIH possesses a major component of approximately 34000 Da against which most of the sera from grass-sensitive patients and none from non-atopic volunteers contain specific IgE antibodies. Monoclonal antibodies to rye Group I were raised and used to purify the antigen and to verify the cross-reactivity between grass extracts. The 3 monoclonal antibodies studied recognized different components of timothy grass and 2 of them recognized kentucky june grass but none recognized components of ragweed extracts.
Mourad
W
W
Pelletier
G
G
Boulet
A
A
Islam
N
N
Valet
J P
JP
Hébert
J
J
D000485:Allergens / Q000276:immunology*; D000911:Antibodies, Monoclonal; D003429:Cross Reactions; D002523:Edible Grain / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D007073:Immunoglobulin E / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007163:Immunosorbent Techniques; D008970:Molecular Weight; D006109:Poaceae / Q000276:immunology*; D011058:Pollen / Q000276:immunology*; D012434:Secale / Q000276:immunology*
D000485:Allergens; D000911:Antibodies, Monoclonal; D000939:Epitopes; D007074:Immunoglobulin G; D007073:Immunoglobulin E