A new human ovarian carcinoma cell line: establishment and analysis of tumor-associated markers.
In the present study we describe the establishment and characteristics of a new human tumor cell line (OV-1063) positive for carcinoembryonic antigen (CEA) originating from ovarian metastatic tumor cells. Analysis of the cultured cells during their in vitro adaptation period revealed while the primary culture exhibited a low proportion of CEA-positive cells, this proportion increased with culture passages and eventually more than 90% of the cells in the established line were CEA-positive. Thus, during the period of adaptation to in vitro growth, a selection for CEA-positive cells took place but the amount of CEA secreted per each positive cell seemed to be constant. Several tumor-associated characteristics were found positive on the established OV-1063 cell line. The in vitro growing cell line exhibited an abnormal chromosome pattern with a near-trisomy karyotype for some chromosomes, colony formation in soft agar as well as positive staining with a monoclonal antibody B38.1. Culture supernatants of the OV-1063 cells contained significant amounts of CEA as well as CA-125 antigen which is an ovarian-carcinoma-associated antigen.
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Horowitz
A J
AJ
Treves
R
R
Voss
E
E
Okon
Z
Z
Fuks
L
L
Davidson
S
S
Biran
D000362:Agar; D000951:Antigens, Neoplasm / Q000032:analysis; D015295:Antigens, Tumor-Associated, Carbohydrate; D001202:Ascitic Fluid / Q000473:pathology; D002272:Carcinoembryonic Antigen / Q000032:analysis; D002460:Cell Line; D003470:Culture Media / Q000032:analysis; D003536:Cystadenocarcinoma / Q000276:immunology / Q000473:pathology* / Q000648:ultrastructure; D005260:Female; D006801:Humans; D007621:Karyotyping; D007633:Keratins / Q000032:analysis; D008858:Microscopy, Phase-Contrast; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000276:immunology / Q000473:pathology* / Q000648:ultrastructure
D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D002272:Carcinoembryonic Antigen; D003470:Culture Media; D007633:Keratins; D000362:Agar