Analysis of hen egg white lysozyme (HEL)-specific delayed type hypersensitivity hybridomas.
Hen egg white lysozyme (HEL) specific delayed type hypersensitivity hybridomas (TDTH hybridomas) were established by fusing HEL specific C3H/HeN (H-2k haplotype) TDTH with BW5147 thymoma cells. TDTH hybridomas, which had Lyt-1+2- and Thy-1.2 antigens on their surfaces, were injected into mouse footpads with HEL antigens, and foot pad swellings were measured 24 h later. Mice with I-Ak haplotype showed strong responses, and their swellings were reduced by anti-I-Ak specific monoclonal antibodies, which suggests that the activity of the hybridomas is restricted by I-Ak. However, TDTH hybridomas did not modulate HEL specific T-cell proliferation responses.
Ishii
N
N
Aoki
I
I
Aihara
Y
Y
Hikawa
N
N
Takahashi
T
T
Okuda
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000032:analysis; D000939:Epitopes; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D006825:Hybridomas / Q000276:immunology*; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007700:Kinetics; D008213:Lymphocyte Activation; D051379:Mice; D008815:Mice, Inbred Strains / Q000276:immunology; D009113:Muramidase / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D000939:Epitopes; D000949:Histocompatibility Antigens Class II; D009113:Muramidase