The action of actinomycin D on the transcription of T7 coliphage DNA by Escherichia coli RNA polymerase.
An actinomycin D molecule bound to DNA sometimes stops the synthesis of RNA by Escherichia coli RNA polymerase. However, quite often, the bound antibiotic is released before the RNA polymerase detaches from the template DNA, so that the enzyme can resume, without interruption, the synthesis of the RNA chain.
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D004279:DNA, Viral / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D003609:Dactinomycin / Q000494:pharmacology*; D004926:Escherichia coli / Q000201:enzymology; D012313:RNA / Q000096:biosynthesis; D013604:T-Phages / Q000235:genetics*; D014158:Transcription, Genetic / Q000187:drug effects*
D004279:DNA, Viral; D003609:Dactinomycin; D012313:RNA; D012321:DNA-Directed RNA Polymerases