Control of synthesis and expression of H-2 heavy chain and beta-2 microglobulin in AKR leukemias.
In a series of newly isolated AKR leukemias, some tumors expressed large amounts of both H-2K and H-2D molecules, while others had reduced levels of both antigens. The number of H-2 antigens expressed in steady state showed a consistent correlation with the rate of synthesis of the H-2 class I heavy chain and the beta-2 microglobulin (beta 2m) light chain, and with the amount of H-2- and beta 2m-specific mRNA present in the tumors. Stimulation of leukemic cells with interferon induced an increased transcription of both H-2 and beta 2m mRNA. These results suggest that there is a mechanism that regulates, at the transcriptional level, the coordinate expression of H-2K and H-2D heavy chains, and the beta 2m light chains encoded by genes on chromosomes 17 and 2, respectively.
Schmidt
W
W
Henseling
U
U
Bevec
D
D
Alonzo
A D
AD
Festenstein
H
H
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002460:Cell Line; D006183:H-2 Antigens / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D007143:Immunoglobulin Heavy Chains / Q000032:analysis / Q000096:biosynthesis*; D007372:Interferons / Q000494:pharmacology; D007942:Leukemia, Experimental / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008806:Mice, Inbred AKR; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic; D001613:beta 2-Microglobulin / Q000032:analysis / Q000096:biosynthesis*
D000954:Antigens, Surface; D006183:H-2 Antigens; C024755:H-2K(K) antigen; D007143:Immunoglobulin Heavy Chains; D012333:RNA, Messenger; D001613:beta 2-Microglobulin; D007372:Interferons