[Physico-chemical characteristics of pregnancy-specific protein in the rat].
Molecular weight of PSP, determined by gel-filtration on Sephadex G-200, is 130 000 +/- 4 000 D and, according to the Laemmli method with SDS, 85 000 +/- 2 000 D. The protein was detected in the zone of beta 1-globulins, its relative electrophoretic mobility is 0.45 +/- 0,2. PSP is heterogeneous by electric charge; at electrofocusing this protein was revealed in the following isoelectric points (pI); 5.85, 6.11, and 6.57. PSP was precipitated with ammonium sulfate (20-50% of saturation). Iron, carbohydrates, lipids were not found histochemically in PSP and it did not manifest the esterase and phosphatase activity.
S K
SK
Krivonosov
Tatarinov Ius
L A
LA
Ivkova
D000818:Animals; D001609:Beta-Globulins / Q000032:analysis*; D005260:Female; D008970:Molecular Weight; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000032:analysis*; D011268:Pregnancy-Specific beta 1-Glycoproteins / Q000032:analysis*; D051381:Rats
D001609:Beta-Globulins; D011257:Pregnancy Proteins; D011268:Pregnancy-Specific beta 1-Glycoproteins
Fiziko-khimicheskaia kharakteristika spetsificheskogo belka beremennosti krys.