A high performance liquid chromatographic assay for tocainide with alternate application for the determination of lidocaine, procainamide, and N-acetylprocainamide.
We describe a simple, isocratic high-performance liquid chromatographic method for measuring the oral antiarrhythmic agent tocainide in serum. The extraction requires 200 microliters of serum, is performed on a reverse-phase column, and utilizes the drug mepivacaine as the internal standard. This analytical method can also be used to assay procainamide, n-acetylprocainamide, or lidocaine with minor modifications to either the extraction protocol or the wavelength used to monitor column eluant.
Annesley
T
T
Matz
K
K
Davenport
R
R
Giacherio
D
D
D000120:Acecainide / Q000097:blood; D000284:Administration, Oral; D000889:Anti-Arrhythmia Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008012:Lidocaine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood; D011342:Procainamide / Q000097:blood; D016677:Tocainide
D000889:Anti-Arrhythmia Agents; D016677:Tocainide; D000120:Acecainide; D008012:Lidocaine; D011342:Procainamide