Treatment of baboon with vinblastine: insights into the mechanisms of pharmacologic stimulation of Hb F in the adult.
Vinblastine was administered to anemic baboons to test whether stimulation of Hb F takes place following distortion of erythropoiesis by an M-stage-specific compound. The treatments elicited erythroid cell cytoreduction followed by regeneration. During the phase of reticulocyte reduction, gamma/gamma + beta biosynthetic ratios increased without increment in F reticulocytes, suggesting that there was increased production of Hb F per F cell. The phase of reticulocyte regeneration was associated with sharp increments in relative (percentage) and absolute F reticulocytes. These data suggest that perturbations of erythropoiesis underlie the stimulation of Hb F synthesis by vinblastine. Accelerated or abnormal precursor maturation may account for the release of shift F reticulocytes with higher Hb F content, during the reduction phase. Accelerated total erythroid differentiation/maturation may account for the increment in F reticulocyte numbers during the phase of regeneneration.
R
R
Veith
T
T
Papayannopoulou
S
S
Kurachi
G
G
Stamatoyannopoulos
D000740:Anemia / Q000097:blood / Q000503:physiopathology*; D000818:Animals; D002478:Cells, Cultured; D004906:Erythrocyte Count; D004920:Erythropoiesis / Q000187:drug effects; D005319:Fetal Hemoglobin / Q000096:biosynthesis*; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D010215:Papio; D012156:Reticulocytes / Q000187:drug effects / Q000378:metabolism; D014747:Vinblastine / Q000494:pharmacology*
D014747:Vinblastine; D005319:Fetal Hemoglobin