Improved detection of helix-turn-helix DNA-binding motifs in protein sequences.
We present an update of our method for systematic detection and evaluation of potential helix-turn-helix DNA-binding motifs in protein sequences [Dodd, I. and Egan, J. B. (1987) J. Mol. Biol. 194, 557-564]. The new method is considerably more powerful, detecting approximately 50% more likely helix-turn-helix sequences without an increase in false predictions. This improvement is due almost entirely to the use of a much larger reference set of 91 presumed helix-turn-helix sequences. The scoring matrix derived from this reference set has been calibrated against a large protein sequence database so that the score obtained by a sequence can be used to give a practical estimation of the probability that the sequence is a helix-turn-helix motif.
Dodd
I B
IB
Department of Biochemistry, University of Adelaide, Australia.
Egan
J B
JB
D000595:Amino Acid Sequence; D000818:Animals; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D007256:Information Systems; D008969:Molecular Sequence Data; D011336:Probability; D011487:Protein Conformation
D004268:DNA-Binding Proteins; D004247:DNA