A cell surface ELISA for detection of blood group antibodies- on anti-ABO and -MN blood group antibodies.
A cell surface ELISA method was employed for detection of polyclonal anti-A, -B, -M and -N, and monoclonal anti-M and -N antibodies. Native erythrocyte membranes or those fixed with glutaraldehyde (GA) were used as solid phase antigen in the ELISA method. In detection of anti-A and -B, and of monoclonal anti-M and -N antibodies, the method used here on the plates with erythrocyte membranes without fixation of GA had approximately 20-40 fold higher sensitivity than the hemagglutination test, but polyclonal anti-M and -N antibodies almost equally bound on the plates coated with unfixed group-M erythrocyte membranes. Anti-A, -B, and monoclonal anti-M antibodies were detected to the same extent in the plates with fixed erythrocyte membranes as in the plates coated with unfixed erythrocyte membranes, but binding of monoclonal anti-N antibody to group-N fixed erythrocyte membranes was not observed. In regard to the eluted antibody, the present cell surface ELISA method had an advantage over the hemagglutination test in detection of anti-A, -B, and monoclonal anti-M antibodies. Binding of monoclonal anti-N antibody to group-N antigen on the group-N or -MN bloodstains was not observed in both the present ELISA and the hemagglutination test. The results obtained indicate that present ELISA method is very useful to medicolegal practice.
Department of Forensic Science, Saga Medical School, Japan.
K
K
Sodesaki
D000017:ABO Blood-Group System / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D001801:Blood Stains; D004797:Enzyme-Linked Immunosorbent Assay; D004910:Erythrocyte Membrane / Q000276:immunology*; D006801:Humans; D007518:Isoantibodies / Q000032:analysis*; D008951:MNSs Blood-Group System / Q000276:immunology*; D011817:Rabbits
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D007518:Isoantibodies; D008951:MNSs Blood-Group System