On the relationship between human interferon alpha 1 and beta 1 genes.
The human interferon alpha 1 and beta 1 (IFN-alpha 1 and -beta 1) genes are approximately 56% identical in the nucleotide sequence across the regions coding for the mature proteins. An IFN-alpha 1 cDNA insert containing such sequences can be used to isolate 15 IFN-beta 1 cDNA colonies present in a 2600-strong library in Escherichia coli prepared from polyadenylated RNA extracted from human diploid fibroblasts (FS-4) induced with poly(I).poly(C). Conversely, a clone containing an IFN-alpha 1 cDNA exhibited detectable hybridization with an IFN-beta 1 probe. One of the IFN-beta 1 cDNA clones isolated in these experiments had lost the internal PstI and PvuII sites due to a change at nucleotide 277 from a C to an A. As a consequence, this allele codes for Met-47 instead of Leu-47. Suggestive evidence has been obtained for the presence of this allele in human genomic DNA (IFN-beta 1 Met-47).
L T
LT
May
P B
PB
Sehgal
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000378:metabolism; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D005796:Genes / Q000187:drug effects; D006801:Humans; D007370:Interferon Type I / Q000235:genetics*; D011061:Poly A / Q000235:genetics; D011070:Poly I-C / Q000494:pharmacology; D021382:Protein Sorting Signals / Q000378:metabolism; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger
D004251:DNA Transposable Elements; D007370:Interferon Type I; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA; D011070:Poly I-C