Secretory differentiation of human fetal bronchial epithelial cells in culture. A study by histochemistry and electron microscopy.
Human fetal bronchial epithelial (HFBE) cells at 6-8 passages were cultivated on a collagen gel for 10 days. A basal differentiative medium (BDM), consisting of RPMI 1640 supplemented with hormones and growth factors, was employed. Histochemistry, scanning electron microscopy and transmission electron microscopy revealed that HFBE cells developed secretory granules when cultivated on collagen gel in BDM. They were electron-dense and stained positive for PAS but negative for alcian blue. On additional treatment with 8 micrograms/ml vitamin A (VA), the number of secretory granules was increased. Moreover, the HFBE cells lost their surface microvilli, and dilation of rough endoplasmic reticulum was more marked than in culture without VA.
Institut für Experimentelle Pathologie, Medizinische Hochschule Hannover, FRG.
C
C
Brockmeyer
M
M
Emura
A
A
Ochiai
M
M
Riebe
U
U
Mohr
J
J
Hilfrich
D001980:Bronchi / Q000166:cytology / Q000196:embryology*; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology; D003594:Cytoplasmic Granules / Q000648:ultrastructure*; D004847:Epithelial Cells; D004848:Epithelium / Q000196:embryology; D005333:Fetus / Q000166:cytology; D005782:Gels; D006651:Histocytochemistry; D006801:Humans; D008854:Microscopy, Electron
D005782:Gels; D003094:Collagen