Numerical analysis of electrophoretic periplasmic protein patterns, a possible marker system for epidemiologic studies.
The whole-cell and periplasmic protein (PP) compositions of 22 Serratia marcescens isolates were examined. Numerical analysis of whole-cell protein patterns was not a useful procedure for measuring relationships between organisms at the subspecific level. However, there was a very good correlation between electrophoretic PP pattern results and those obtained previously from multilocus enzyme electrophoresis (electrophoretic type) and biotype (D. Gargallo-Viola, J. Clin. Microbiol. 27:860-868, 1989). Clustering of isolates by using PP patterns compared by coefficients based on peak position (Dice coefficient) gave more precise information than that obtained by correlation coefficients. PP patterns appeared to be a useful tool that may be of value for epidemiologic studies.
Gargallo-Viola
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Departament de Microbiologia i Parasitologia Sanitàries, Facultat de Farmàcia, Universitat de Barcelona, Spain.
López
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D001426:Bacterial Proteins / Q000302:isolation & purification*; D015415:Biomarkers; D004591:Electrophoresis, Polyacrylamide Gel; D004756:Enterobacteriaceae Infections / Q000453:epidemiology; D004812:Epidemiologic Methods; D006801:Humans; D008565:Membrane Proteins / Q000302:isolation & purification; D009716:Numerical Analysis, Computer-Assisted; D012706:Serratia marcescens / Q000032:analysis*
D001426:Bacterial Proteins; D015415:Biomarkers; D008565:Membrane Proteins