Inhibitory effect of lysophosphatidylcholine on the histamine release from rat peritoneal mast cells.
Histamine release from isolated rat peritoneal mast cells induced by compound 48/80 (0.5 microgram/ml) or antigen-antibody reaction was inhibited by lysophosphatidylcholine in a dose-dependent fashion at concentrations up to 4 microM. Within the same range of concentration, lysophosphatidylcholine exhibited a membrane-stabilizing action on the model membrane systems decreasing the permeability of lipid bilayer and the fluidity of liposomal membrane in the liquid crystalline state. At concentrations higher than 8 microM, lysophosphatidylcholine damaged the cell membrane and subsequently histamine was released. It was assumed that lysophosphatidylcholine may act as an endogenous membrane stabilizer inhibiting histamine release in normal mast cells.
Mio
M
M
Ikeda
A
A
Akagi
M
M
Tasaka
K
K
D000818:Animals; D001201:Ascites; D002118:Calcium / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006461:Hemolysis / Q000187:drug effects; D006636:Histamine Release / Q000187:drug effects*; D066298:In Vitro Techniques; D008051:Lipid Bilayers; D008081:Liposomes; D008244:Lysophosphatidylcholines / Q000494:pharmacology*; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D008051:Lipid Bilayers; D008081:Liposomes; D008244:Lysophosphatidylcholines; D003189:p-Methoxy-N-methylphenethylamine; D002118:Calcium