[Role of microtubules and intermediate filaments in maintaining the shape of epithelial cells].
The role of microtubules and intermediate filaments in control of cell shape of cultured cells of hepatomas McA-RH-7777 and 27 was investigated. Indirect immunofluorescence with specific polyclonal antibodies against tubulin and monoclonal antibodies against prekeratin with molecular weight 49 kD and vimentin was used. Incubation of cells in colcemid, resulting in specific distribution of microtubules did not change either prekeratin or vimentin distribution in cells of both the hepatomas, but reversed polarization of elongated McA-RH-7777 cells. These data suggest that the effect of disruption of microtubular system on the cell shape is not mediated by alterations of intermediate filaments.
Karavanova
I D
ID
Vasil'ev
Iu M
IuM
Troianovskiĭ
S M
SM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000187:drug effects / Q000648:ultrastructure*; D003703:Demecolcine / Q000494:pharmacology; D004848:Epithelium / Q000187:drug effects / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D007381:Intermediate Filament Proteins / Q000276:immunology; D007633:Keratins / Q000276:immunology; D008114:Liver Neoplasms, Experimental / Q000648:ultrastructure*; D008870:Microtubules / Q000187:drug effects / Q000648:ultrastructure*; D009368:Neoplasm Transplantation; D011498:Protein Precursors / Q000276:immunology; D051381:Rats; D014404:Tubulin / Q000276:immunology; D014746:Vimentin / Q000276:immunology
D000911:Antibodies, Monoclonal; D007381:Intermediate Filament Proteins; D011498:Protein Precursors; D014404:Tubulin; D014746:Vimentin; C004818:prekeratin; D007633:Keratins; D003703:Demecolcine
Rol' mikrotrubochek i promezhutochnykh filamentov v podderzhanii formy épitelial'nykh kletok.