Local and systemic effects of organ hypoxia detected by chemiluminescence and photochemiluminescence.
Perfusate, blood plasma and tissue homogenate were investigated by H2O2-stimulated chemiluminescence (CL) and photochemiluminescence (PCL) during experimental liver and kidney transplantation. The canine kidneys were perfused prior to reimplantation for 68 h at 4 degrees C. The increase of the H2O2 CL of the perfusate correlated positively with the LDH activity. After ischemic load of the liver, the antioxidative capacity (AC) of the tissue as determined by PCL decreases. After transplantation of the stored liver (1 h, 4 degrees C) an increased AC is observed in peripheral blood. This increase is lower after addition of ascorbate to the preservation solution. The results allow to conclude that both methods are suitable for the characterization of the organ status after hypoxic injury during conservation.
Popov
I
I
Institute of Medical Immunology, Faculty of Medicine (Charité), Humboldt University, Berlin, GDR.
Lewin
G
G
Gäbel
W
W
von Baehr
R
R
D000818:Animals; D015687:Cell Hypoxia; D003404:Creatinine / Q000032:analysis; D004285:Dogs; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007668:Kidney / Q000378:metabolism*; D016030:Kidney Transplantation; D015227:Lipid Peroxidation; D008099:Liver / Q000378:metabolism*; D016031:Liver Transplantation; D008163:Luminescent Measurements; D008165:Luminol / Q000528:radiation effects; D009924:Organ Culture Techniques; D009926:Organ Preservation; D010084:Oxidation-Reduction; D013552:Swine; D013556:Swine, Miniature; D014182:Transplantation, Autologous
D005609:Free Radicals; D008165:Luminol; D003404:Creatinine; D006861:Hydrogen Peroxide