Isolation and characterisation of human T-cell lines from a patient with Chagas' disease.
Short-term T-cell lines reactive to different Trypanosoma cruzi antigens were isolated from a patient with Chagas' disease. These T-cell lines were analysed phenotypically with monoclonal antibodies defining pan-T (T11), helper (T4), or cytotoxic/suppressor epitopes by the use of a continuous-flow microfluorimeter. One cell line, 5C3, was obtained from T-cell blasts reactive to formaldehyde-fixed amastigotes (the intracellular stage of the parasite) plated at limiting dilution (3.3 cells/well). This line was shown to be T11 and T4 positive, to respond to specific antigen in an HLA-DR restricted manner, and to produce interleukin-2 under similar growth conditions.
Britten
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Hudson
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D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000276:immunology; D002460:Cell Line; D014355:Chagas Disease / Q000276:immunology*; D000939:Epitopes; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D013601:T-Lymphocytes / Q000145:classification* / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000145:classification / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000145:classification / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000145:classification / Q000276:immunology; D014349:Trypanosoma cruzi / Q000276:immunology
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D000939:Epitopes; D007376:Interleukin-2