[Effect of dye quality and the method of preparing a Schiff reagent on the staining intensity and DNA-fuchsin absorption spectra in performing the Feulgen reaction].
Dependence of the staining and absorption spectra of DNA-fuchsin on the dye quality and modes of preparation of the Schiff-reagent was studied. It is shown that many dyes supplied by different producers do not suit the purposes of quantitative cytochemical investigations. Before use the absorption spectra of the DNA-fuchsin must be taken using the standard object. The use of dyes possessing a multi-peak absorption curve should be avoided.
Magakian
Iu A
IuA
Avetisian
A S
AS
Karalova
E M
EM
D000042:Absorption; D000818:Animals; D002645:Chickens; D004396:Coloring Agents / Q000378:metabolism*; D004247:DNA / Q000032:analysis / Q000378:metabolism*; D006651:Histocytochemistry; D007202:Indicators and Reagents / Q000494:pharmacology; D008297:Male; D011786:Quality Control; D051381:Rats; D012394:Rosaniline Dyes / Q000032:analysis / Q000378:metabolism*; D013053:Spectrophotometry; D013194:Staining and Labeling / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000494:pharmacology*
D004396:Coloring Agents; C007722:Feulgen stain; D007202:Indicators and Reagents; D012394:Rosaniline Dyes; D013438:Sulfhydryl Compounds; D004247:DNA; C005732:thioacetic acid
Vliianie kachestva krasitelia i sposoba prigotovleniia reaktiva Shiffa na intensivnost' okrashivaniia i spektry pogloshcheniia DNK-fuksina pri provedenii reaktsii Fël'gena.