[Proteins of bee honey. VI. Isoelectric focusing of amylase in various kinds of honey].
The isolated honey amylase was characterized by preparative and analytical isoelectric focusing. An interesting aspect of the preparative isoelectric focusing was the reduction of iodine by Ampholines which were used for building up a pH-gradient, giving a false increase in apparent amylase activity as determined by the iodine/starch reaction. Dextran gels were not suited for the matrix, because interactions with the enzyme disturb the focusing. From the analytical isoelectric focusing of isolated honey amylase in ultrathin layer Polyacrylamide gels 10 protein bands resulted. A newly developed zymogram technique was positive for amylase activity in all 10 protein bands.
Stadelmeier
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Bergner
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D000681:Amylases / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D006722:Honey / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007525:Isoelectric Focusing
D007202:Indicators and Reagents; D000681:Amylases
Proteine des Bienenhonigs. VI. Isoelektrische Focussierung der Amylase verschiedener Honigsorten.