Stopped-flow studies of cytochrome oxidase reconstituted into liposomes: proton pumping and control of activity.
The transient kinetics of proton pumping and the electron transfer properties of cytochrome oxidase inserted into small unilamellar vesicles have been investigated by stopped-flow spectrophotometry. In the presence of valinomycin, proton pumping and cytochrome c oxidation by cytochrome oxidase are synchronous up to rate constants of approximately 9 sec-1. Moreover, the enzyme depleted of subunit III ("three-less oxidase") was also shown to pump protons, although with a significantly smaller stoichiometry. Thus, subunit III is not the only (or even the main) proton channel, although it may be involved in the regulation of activity. The kinetics of cytochrome c oxidation by COV in the absence and in the presence of ionophores have been investigated. Analysis of the time course of the process in the transient and steady state phases indicates that the onset of control by the electrochemical gradient follows the transfer of four electrons, i.e., one complete turnover of the oxidase. Two possible alternative interpretations for the control of the turnover phase are presented and discussed.
M
M
Brunori
G
G
Antonini
A
A
Colosimo
F
F
Malatesta
P
P
Sarti
M G
MG
Jones
M T
MT
Wilson
D000818:Animals; D002417:Cattle; D003574:Cytochrome c Group / Q000378:metabolism; D004579:Electron Transport; D003576:Electron Transport Complex IV / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008081:Liposomes / Q000378:metabolism*; D009206:Myocardium / Q000201:enzymology; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D011522:Protons; D014634:Valinomycin / Q000494:pharmacology
D003574:Cytochrome c Group; D007473:Ion Channels; D008081:Liposomes; D011522:Protons; D014634:Valinomycin; D003576:Electron Transport Complex IV