Monocyte subpopulations in human gliomas: expression of Fc and complement receptors and correlation with tumor proliferation.
Cryostat sections of 12 gliomas and of 3 peritumoral brain tissue samples were investigated for mononuclear cell infiltration by immunohistochemistry, concentrating on cells expressing monocyte/macrophage markers. Only low numbers of T cells were detected in the tumors, whereas in average 20%-30% of all cells present in the samples were recognized by various macrophage markers. These cells carried surface epitopes with known function, like Fc-gamma (Fcg) and complement receptors. Microglial cells, in comparison to typical debris laden macrophages, were only recognized by a restricted panel of macrophages markers (anti-Fcg receptors 1, 2, 3, complement receptor CR3, HLA DR, common leucocyte antigen CD45 and the monocyte marker RM3/1). In peritumoral tissue mainly dendritic, microglia-like cells were present, which revealed decreased expression of antigens CD4, RM3/1 and Fcg receptors in comparison to those in gliomas. A significant positive correlation was found between the number of RM3/1 or CR3 (CD11b)-positive cells and the proliferation rate of the tumors as documented by the number of bromodeoxyuridine-positive or Ki-67+ cells.
Morimura
T
T
Neurologisches Institut, Universität Wien, Austria.
Neuchrist
C
C
Kitz
K
K
Budka
H
H
Scheiner
O
O
Kraft
D
D
Lassmann
H
H
D015703:Antigens, CD / Q000032:analysis; D001932:Brain Neoplasms / Q000276:immunology / Q000473:pathology*; D002455:Cell Division; D005910:Glioma / Q000276:immunology / Q000473:pathology*; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D011951:Receptors, Complement / Q000032:analysis; D011961:Receptors, Fc / Q000032:analysis
D015703:Antigens, CD; D006684:HLA-DR Antigens; D011951:Receptors, Complement; D011961:Receptors, Fc