Calcium efflux changes in neutrophils from patients with severe atopic dermatitis.
In seven patients with severe atopic dermatitis (AD) we investigated the 45Ca-efflux responses from prelabelled neutrophils stimulated with the chemotactic peptide formyl-methionyl-leucyl-phenylalanine (F-Met-Leu-Phe) and catecholamines. We found no difference between the burst in 45Ca-efflux induced by F-Met-leu-Phe in AD patients when comparing with controls. Alpha 1-receptor responses are believed to be mediated by a calcium mechanism and like normal cells the 45Ca-efflux rate in AD neutrophils was unchanged both after stimulation with alpha- and beta-receptor agonists. Whereas the cAMP-hyporesponsiveness of beta-adrenergic receptors is known in atopic disease our present study might indicate a normal alpha-adrenergic response in AD neutrophils.
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T
Herlin
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Ternowitz
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Kragballe
D000293:Adolescent; D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000328:Adult; D002118:Calcium / Q000378:metabolism*; D002395:Catecholamines / Q000494:pharmacology; D003876:Dermatitis, Atopic / Q000097:blood / Q000378:metabolism*; D005260:Female; D006801:Humans; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000378:metabolism*; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism; D013268:Stimulation, Chemical
D000316:Adrenergic alpha-Agonists; D000318:Adrenergic beta-Agonists; D002395:Catecholamines; D007473:Ion Channels; D011942:Receptors, Adrenergic, alpha; D009240:N-Formylmethionine Leucyl-Phenylalanine; D002118:Calcium