Selective killing of smooth muscle cells in culture by the ricin A-chain conjugated with monoclonal antibodies to a cell surface antigen via a dextran bridge.
Monoclonal antibodies to a surface antigen of the modulated smooth muscle cells originally isolated from the rat aorta media were conjugated with ricin A-chain via an oxidized dextran bridge. The interaction of cultured cells with the conjugates obtained and with control substances was monitored following incorporation of 14C-leucine radioactivity. It was found that 14C-leucine incorporation was suppressed by 80-90% at a conjugate concentration of 10(-6)-10(-7) M. Antigen-negative cells (line IAR; rat hepatocytes) were insensitive to the conjugate at any concentration used. Control use of purified ricin A-chain, native or oxidized dextran, specific and nonspecific IgG did not affect normal 14C-leucine incorporation. The data obtained may be useful for designing targeted drug transport systems and for selective screening of modulated smooth cells in vascular pathology models in vivo.
Printseva OYu
Faerman
A I
AI
Maksimenko
A V
AV
Tonevitsky
A G
AG
Ilynsky
O B
OB
Torchilin
V P
VP
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000276:immunology; D001011:Aorta / Q000166:cytology; D002470:Cell Survival / Q000187:drug effects*; D002850:Chromatography, Gel; D003911:Dextrans; D007930:Leucine / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D012276:Ricin / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D003911:Dextrans; D012276:Ricin; D007930:Leucine