The membrane-anchoring systems of vertebrate acetylcholinesterase and variant surface glycoproteins of African trypanosomes share a common antigenic determinant.
Amphiphilic detergent-soluble acetylcholinesterase (AChE) from Torpedo is converted to a hydrophilic form by digestion with phospholipase C from Trypanosoma brucei or from Bacillus cereus. This lipase digestion uncovers an immunological determinant which crossreacts with a complex carbohydrate structure present in the hydrophilic form of all variant surface glycoproteins (VSG) of T. brucei. This crossreacting determinant is also detected in human erythrocyte AChE after digestion with T. brucei lipase. From these results we conclude that the glycophospholipid anchors of protozoan VSG and of AChE of the two vertebrates share common structural features, suggesting that this novel type of membrane anchor has been conserved during evolution.
A
A
Stieger
M L
ML
Cardoso de Almeida
M C
MC
Blatter
U
U
Brodbeck
C
C
Bordier
D000110:Acetylcholinesterase / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D001409:Bacillus cereus / Q000201:enzymology; D002462:Cell Membrane / Q000201:enzymology; D004557:Electric Organ / Q000201:enzymology; D000939:Epitopes / Q000032:analysis*; D004910:Erythrocyte Membrane / Q000201:enzymology*; D006023:Glycoproteins / Q000276:immunology*; D054800:Glycosylphosphatidylinositol Diacylglycerol-Lyase; D006801:Humans; D007700:Kinetics; D010740:Phospholipases / Q000378:metabolism; D014101:Torpedo; D014346:Trypanosoma brucei brucei / Q000201:enzymology / Q000276:immunology*; D010738:Type C Phospholipases; D014643:Variant Surface Glycoproteins, Trypanosoma
D000953:Antigens, Protozoan; D000939:Epitopes; D006023:Glycoproteins; D014643:Variant Surface Glycoproteins, Trypanosoma; D010740:Phospholipases; D000110:Acetylcholinesterase; D010738:Type C Phospholipases; D054800:Glycosylphosphatidylinositol Diacylglycerol-Lyase