Somatic mutations in the variable region of the lambda 2 chain of M315 suppression of antibodies to the idiotype of M315.
BALB/c mice immunized with purified BALB/c myeloma protein M315 (alpha, lambda 2) produce anti-idiotypic antibody directed predominantly to a combinational (VH-315 + VL-315) determinant(s) of the M315 paratope (Sirisinha and Eisen, 1971; Tungkanak and Sirisinha, 1976). We examined whether the unique B cell response is influenced by pretreatment of mice with fragments or chains derived from M315 before immunization with M315. Intravenous (i.v.) injection of the Fv-315 fragment (VH-315 + VL-315) into normal BALB/c mice seven days before immunization with M315 resulted in marked suppression of anti-M315 idiotype antibodies. Studies on the structural requirement for suppression indicated that VL-315, but not VH-315, is involved. Structural comparison with a defined lambda 2 light (L) chain suggested that three contiguous amino acid residues in the third hypervariable loop of the variable (V) domain of the L chain of M315 are important for down-regulation of production of antibodies to the M315 idiotype.
Sakato
N
N
Azuma
T
T
Fujio
H
H
D000818:Animals; D000917:Antibody Formation; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D005260:Female; D007114:Immunization; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007147:Immunoglobulin Light Chains / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007146:Immunoglobulin lambda-Chains / Q000276:immunology*; D007274:Injections, Intraperitoneal; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008815:Mice, Inbred Strains; D009154:Mutation; D009194:Myeloma Proteins / Q000008:administration & dosage / Q000276:immunology*; D013491:Suppressor Factors, Immunologic
D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D007146:Immunoglobulin lambda-Chains; D009194:Myeloma Proteins; D013491:Suppressor Factors, Immunologic