A monoclonal antibody against altered LFA-1 induces proliferation and lymphokine release of cloned T cells.
A murine monoclonal antibody (I-17, IgM) has the following functional effects on murine long-term T cell clones: inhibition of cell-mediated lysis, induction of proliferation, release of lymphokines and change of the cell morphology. The determinant detected by I-17 is expressed on long-term T lines but not on thymocytes, lymph node cells and spleen cells. I-17 precipitated proteins with apparent molecular mass of 220 kDa, 170 kDa, 150 kDa and 100 kDa. Biochemical studies indicate that the determinant recognized by I-17 is tunicamycin sensitive and that I-17 binds to the alpha chain of the lymphocyte function-associated antigen (LFA-1).
H
H
Pircher
P
P
Groscurth
S
S
Baumhütter
M
M
Aguet
R M
RM
Zinkernagel
H
H
Hengartner
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology*; D002448:Cell Adhesion; D011232:Chemical Precipitation; D002999:Clone Cells; D003208:Concanavalin A / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000032:analysis; D005260:Female; D008213:Lymphocyte Activation; D016169:Lymphocyte Function-Associated Antigen-1; D008222:Lymphokines / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011487:Protein Conformation; D013601:T-Lymphocytes / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000648:ultrastructure; D014415:Tunicamycin / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000939:Epitopes; D016169:Lymphocyte Function-Associated Antigen-1; D008222:Lymphokines; D003208:Concanavalin A; D014415:Tunicamycin