Binding sites for [3H]substance P on neurons of cultured rat spinal cord and brain stem: an autoradiographic study.
Binding of substance P (SP) was studied in organotypic cultures of rat central nervous system by means of autoradiography. In spinal cord cultures, binding sites for [3H]SP were observed on many interneurons located in the dorsal horn, whereas in the ventral horn, mainly large neurons, probably, motoneurons, were labelled. Almost no binding was detected on neurons of attached dorsal root ganglia. Binding of [3H]SP was also found on a relatively great number of brain stem neurons of various sizes. In contrast, glial cells did not reveal binding sites for [3H]SP. These binding studies together with electrophysiological investigations provide strong evidence for the existence of SP receptors on spinal and brain stem neurons but not on glial cells.
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D000818:Animals; D001345:Autoradiography; D001933:Brain Stem / Q000378:metabolism*; D046508:Culture Techniques; D005727:Ganglia, Spinal / Q000378:metabolism; D008125:Locus Coeruleus / Q000378:metabolism; D008526:Medulla Oblongata / Q000378:metabolism; D009928:Organ Specificity; D051381:Rats; D013116:Spinal Cord / Q000378:metabolism*; D013373:Substance P / Q000378:metabolism*
D013373:Substance P