[Effects of solute on growth and intracellular salt concentration of Pediococcus halophilus CCRC 12884 isolated from Inyu mash].
Optimal growth conditions for Pediococcus halophilus CCRC 12884, a halophilic strain of lactic acid bacterium isolated from Inyu (black bean sauce) mash, were MRS broth with 10% NaCl and 35 degrees C of incubation temperature. The NaCl in media could not be replaced by sucrose or glycerol of same concentrations, and under certain osmolarity, growth of P. halophilus CCRC 12884 only in NaCl was better than that in NaCl plus sucrose or glycerol. To determine effects of monovalent and divalent ions on P. halophilus CCRC 12884, it was found that close relations were present among Na+, K+ and PO4(-3). The intracellular concentration of Na+ in P. halophilus CCRC 12884 was not correspondent to its extracellular concentration of NaCl, but intracellular K+ and quaternary ammonium compounds significantly increased as extracellular NaCl increased. Adding 0-3.5 mM betaine to media with different contents of NaCl would not affect the growth of this halophilic bacterium. On feeding 14C-ethanolamine to the bacterium, no intracellular 14C-betaine was synthesized.
Culture Collection and Research Center, Food Industry, Research and Development Institute, Hsinchu, Taiwan, R.O.C.
L S
LS
Tsao
J J
JJ
Hua
D001622:Betaine / Q000494:pharmacology; D004573:Electrolytes / Q000032:analysis*; D010376:Pediococcus / Q000032:analysis / Q000187:drug effects / Q000254:growth & development*; D012965:Sodium Chloride / Q000494:pharmacology*; D013025:Glycine max
D004573:Electrolytes; D001622:Betaine; D012965:Sodium Chloride