[Interaction of the Latrodectus tredecimguttatus spider venom with liposomes].
It is established that interaction of Latrodectus tredecimguttatus venom with bilayer phospholipid membranes of liposomes results in formation of ion channels. Liposome permeability variations are estimated by input and output of 86Rb+ and 45Ca2+ isotopes. It is shown that LaCl3 in 0.5 mM concentration decreases considerably both input and output of Rb+ in proteoliposomes and it may be used as an inhibitor of latrotoxin channels in experiments with phospholipid vesicles. When studying electrophoretic spectrum of proteins bound with liposomes it is shown that latrotoxin (a polypeptide) with molecular mass 130 kDa predominates in proteoliposomes.
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Trikash
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Lishko
D000818:Animals; D001180:Arthropod Venoms / Q000494:pharmacology*; D001740:Black Widow Spider; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects*; D008081:Liposomes / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism; D010539:Permeability; D013111:Spider Venoms / Q000378:metabolism / Q000494:pharmacology*; D013112:Spiders
D001180:Arthropod Venoms; D007473:Ion Channels; D008081:Liposomes; D008563:Membrane Lipids; D013111:Spider Venoms; C038599:black widow spider venom
Vzaimodeĭstvie iadapauka karakurta Latrodectus tredecimguttatus s liposomami.