Identification of a putative cell adhesion domain of uvomorulin.
A rat monoclonal antibody (DECMA-1) selected against the murine cell adhesion molecule uvomorulin blocks both the aggregation of mouse embryonal carcinoma cells and the compaction of pre-implantation embryos. However, decompacted embryos eventually become recompacted in the presence of DECMA-1 and form blastocysts composed of both trophectoderm and inner cell mass. DECMA-1 also disrupts confluent monolayers of Madin-Darby canine kidney (MDCK) epithelial cells. DECMA-1 recognizes uvomorulin in extracts from mouse and dog tissues. Protease digestion of mouse and dog uvomorulin generated core fragments including one of 26 kd which reacted with DECMA-1. The same 26-kd fragment is recognized by anti-uvomorulin monoclonal antibodies which have been obtained from other laboratories and which dissociate MDCK cell monolayers and block the formation of the epithelial occluding barrier. This 26-kd fragment therefore seems to be involved in the adhesive function of uvomorulin.
Vestweber
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Kemler
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D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000276:immunology / Q000502:physiology*; D015820:Cadherins; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules; D002478:Cells, Cultured; D002970:Cleavage Stage, Ovum / Q000166:cytology*; D004285:Dogs; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000276:immunology / Q000502:physiology*; D051379:Mice; D009028:Morula / Q000166:cytology*; D010446:Peptide Fragments / Q000276:immunology / Q000502:physiology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D015820:Cadherins; D015815:Cell Adhesion Molecules; D000939:Epitopes; D006023:Glycoproteins; D010446:Peptide Fragments