Plasma membrane fluidity studies by fluorescence polarization in rat mast cells stimulated by compound 48/80.
Plasma membrane fluidity measurements were performed on purified living mast cells using a novel non-permeant fluorescence polarization probe TMA-DPH, upon stimulation by compound 48/80. The fluorescence anisotropy increased rapidly after treatment by 48/80 in a dose-dependent way. The effect was found to be specific for mast cells; it was inhibited by the histamine release antagonist FR 7534 in a correlative manner. The role of calcium was examined. The results brought evidence for a plasma membrane fluidity decrease induced by 48/80; a biphasic mechanism was inferred for the histamine release process.
J G
JG
Kuhry
C
C
Bronner
M
M
Amellal
Y
Y
Landry
D000818:Animals; D002118:Calcium / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005454:Fluorescence Polarization; D006636:Histamine Release / Q000187:drug effects; D008297:Male; D008407:Mast Cells / Q000187:drug effects*; D008560:Membrane Fluidity / Q000187:drug effects*; D009543:Nifedipine / Q000031:analogs & derivatives / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology*
D003189:p-Methoxy-N-methylphenethylamine; C019137:FR 7534; D009543:Nifedipine; D002118:Calcium