A trypsin-like proteinase appearing at 17 h and 17 min in the cell cycle time of HeLa cells correlates with the onset of DNA synthesis.
A trypsin-like proteinase appearing sharply at 17 h 17 min (17:17) in the cell cycle time of the synchronized and growing HeLa cells correlates with the onset of DNA synthesis, and the inhibition of the proteinase activity by trans-4-guanidinomethylcyclohexanecarboxylic acid 4-tert-butylphenyl ester (GMCHA-OPhBut) results in a 3 h retardation of the onset of the DNA synthesis. The proteinase activity is cell density dependent and completely inhibited by 100 microM GMCHA-OPhBut. The proteinase was named HeLa tryptase 17:17. The fact that the DNA synthesis occurred after the 3 h retardation in the presence of GMCHA-OPhBut strongly suggests the involvement of the alternative trigger for DNA synthesis in HeLa cells.
Muramatu
M
M
Faculty of Pharmacy, Tokushima Bunri University, Japan.
Kozaki
Y
Y
D002453:Cell Cycle / Q000187:drug effects*; D003509:Cyclohexanecarboxylic Acids / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis*; D005786:Gene Expression Regulation; D006367:HeLa Cells / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D014357:Trypsin / Q000378:metabolism*
D003509:Cyclohexanecarboxylic Acids; C038303:NCO 650; D004247:DNA; D014357:Trypsin