Photosensitizing dyes and fluorochromes as substitutes for 33258 Hoechst in the fluorescence-plus-Giemsa (FPG) chromosome technique.
Using Allium cepa chromosomes after 5-bromo, 2'-deoxyuridine (BrdU) incorporation, we studied several acid and basic dyes and fluorochromes for their potential as substitutes for 33258 Hoechst in the fluorescence-plus-Giemsa (FPG) technique. All of the dyes and fluorochromes investigated showed a photosensitizing capacity which was slightly lower than 33258 Hoechst in the cases of daunomycin, phloxin, fluorescein, thioflavine T and nuclear fast red, and somewhat higher in the case of eosin Y. Observation and cytophotometric analysis of differentially Giemsa-stained sister chromatids when eosin Y was used as the photosensitizing agent revealed the unsubstituted chromatid to be reddish violet in colour (absorption maximum, 550 nm), while the BrdU-substituted chromatid was blue or pale violet blue (absorption maximum, 580 nm). These results indicate that eosin Y is a useful photosensitizing dye which could be used as a substitute for 33258 Hoechst in the FPG staining technique.
Hazen
M J
MJ
Villanueva
A
A
Juarranz
A
A
Cañete
M
M
Stockert
J C
JC
D006690:Bisbenzimidazole; D001973:Bromodeoxyuridine; D002875:Chromosomes / Q000648:ultrastructure*; D005456:Fluorescent Dyes; D059828:Plant Cells; D013194:Staining and Labeling; D013329:Structure-Activity Relationship
D005456:Fluorescent Dyes; D001973:Bromodeoxyuridine; D006690:Bisbenzimidazole