Highly specific and sensitive hybridoma antibodies against the alpha-subunit of human glycoprotein hormones.
The free alpha-subunit of human CG (hCG-alpha) has been detected in pregnancy as well as in several endocrine and nonendocrine tumors. In order to facilitate the detection of these tumors, we have developed, by hybridoma technology, very sensitive and highly specific antibodies to hCG-alpha, A2D4 and A2-58. Employing these antibodies, assays were developed to detect picogram levels of the free alpha-subunit. These antibodies have negligible cross-reactivity with intact hCG (A2D4, 0.17%; and A2-58, 1.75%). Pituitary hormone standards of human LH, human FSH, and human TSH from NIH showed cross-reactivity with these antibodies. However, the cross-reactivity was found to be due to the contamination of the hormone preparations with alpha-subunit. The antibodies have no effect on the binding of hCG to its receptor. They cross-react to the same extent with the deglycosylated hCG-alpha and alpha-subunits of all other human glycoprotein hormones but do not react with alpha-subunits of glycoprotein hormones from other species. We have purified these antibodies to homogeneity by DEAE Affi-Gel Blue and affinity chromatography. Both these antibodies belong to immunoglobulin M subclass.
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Thotakura
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Bahl
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D000918:Antibody Specificity; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D015292:Glycoprotein Hormones, alpha Subunit; D006801:Humans; D006825:Hybridomas / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D010446:Peptide Fragments / Q000276:immunology*; D010908:Pituitary Hormones, Anterior / Q000276:immunology*; D011863:Radioimmunoassay; D012756:Sheep; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D015292:Glycoprotein Hormones, alpha Subunit; D010446:Peptide Fragments; D010908:Pituitary Hormones, Anterior