Colicin cleavage by OmpT protease during both entry into and release from Escherichia coli cells.
Proteolysis of colicins A, E1, E2, and E3 was observed after they were added to whole cells carrying a functional ompT gene. Recombinant plasmid pML19 containing the ompT gene enabled two mutant strains to cleave the added colicins. On the other hand, two colicin A recombinants were split after release from the wild-type bacteria that produced them but not from ompT mutant cells.
Centre de Biochimie et de Biologie Moléculaire, Centre National de la Recherche Scientifique, Marseille, France.
D
D
Cavard
C
C
Lazdunski
D003087:Colicins / Q000302:isolation & purification / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology*; D015151:Immunoblotting; D012697:Serine Endopeptidases / Q000378:metabolism*
D003087:Colicins; D012697:Serine Endopeptidases; C057840:omptin outer membrane protease