[Isolation of modification-restriction enzymes HpaI and HpaII].
A method for simultaneous isolation of four enzymes of modification-restriction of DNA from Haemophilus parainfluenzae is proposed. The properties of HpaI and HpaII DNA-methylases were investigated.
Bogdarina
I G
IG
Zinkevich
V E
VE
Bur'ianov
Ia I
IaI
Baev
A A
AA
D044503:5-Methylcytosine; D002847:Chromatography, Agarose; D003596:Cytosine / Q000031:analogs & derivatives / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes / Q000302:isolation & purification*; D015257:DNA-Cytosine Methylases; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005697:Galactosides / Q000138:chemical synthesis; D006027:Glycosides; D006190:Haemophilus / Q000201:enzymology*; D006923:Hymecromone / Q000031:analogs & derivatives / Q000138:chemical synthesis; D007700:Kinetics; D008745:Methylation; D008780:Methyltransferases / Q000302:isolation & purification*; D012685:Sepharose / Q000031:analogs & derivatives; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D014468:Umbelliferones
D005697:Galactosides; D006027:Glycosides; D014468:Umbelliferones; C037405:heparin-sepharose; D006923:Hymecromone; D044503:5-Methylcytosine; D003596:Cytosine; D004247:DNA; D012685:Sepharose; C047480:6-hexadecanoylamino-4-methylumbelliferyl-galactopyranoside; C036341:DNA modification methylase HpaI; C029641:DNA modification methylase HpaII; D015257:DNA-Cytosine Methylases; D008780:Methyltransferases; D004248:DNA (Cytosine-5-)-Methyltransferases; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D004262:DNA Restriction Enzymes; D019003:Deoxyribonuclease HpaII; C024406:endodeoxyribonuclease HpaI; D015252:Deoxyribonucleases, Type II Site-Specific
Vydelenie fermentov modifikatsii-restriktsii HpaI i HpaII.