Determination of the distribution of fatty acids and diethylstilbestrol between serum albumin and alpha-fetoprotein by concanavalin A affinity chromatography.
The distribution of fatty acids and diethylstilbestrol between serum albumin and alpha-fetoprotein was measured in vitro by a new method based on the separation of the two proteins by virtue of the binding specificity of concanavalin A for the carbohydrate moiety of alpha-fetoprotein. Human and bovine proteins were investigated. It was found that palmitate and oleate were distributed almost equally between albumin and alpha-fetoprotein, while docosahexaenoate and diethylstilbestrol bound preferentially to alpha-fetoprotein even at an albumin: alpha-fetoprotein ratio of 10:1. The results confirm the binding specificity of alpha-fetoprotein for polyunsaturated fatty acids and also show that alpha-fetoprotein binds diethylstilbestrol much more strongly than albumin does. This suggests that alpha-fetoprotein may play a role in the fetal uptake of diethylstilbestrol.
J C
JC
Hsia
L T
LT
Wong
H F
HF
Deutsch
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002846:Chromatography, Affinity; D003208:Concanavalin A; D004054:Diethylstilbestrol / Q000097:blood*; D004281:Docosahexaenoic Acids; D005227:Fatty Acids / Q000097:blood*; D005231:Fatty Acids, Unsaturated / Q000097:blood; D006801:Humans; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000097:blood; D011485:Protein Binding; D012709:Serum Albumin / Q000032:analysis*; D000509:alpha-Fetoproteins / Q000032:analysis*
D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D010169:Palmitic Acids; D012709:Serum Albumin; D000509:alpha-Fetoproteins; D003208:Concanavalin A; D004281:Docosahexaenoic Acids; D019308:Palmitic Acid; D004054:Diethylstilbestrol