Use of a monoclonal antibody recognizing a cell surface determinant to distinguish prestalk and prespore cells of Dictyostelium discoideum slugs.
Double labelling experiments on Dictyostelium discoideum cells at different developmental stages were carried out using monoclonal antibodies MUD1 (prespore specific), MUD9 (strong label on prestalk and anterior-like cells) and a fluorescence-activated cell sorter. The monoclonal antibody MUD9, which recognizes the surface of prestalk and anterior-like cells strongly and prespore cells weakly, is also present on the surface of vegetative amoebae and on mature stalk cells but not on the spore surface. Sharing of an antigenic determinant between vegetative, prestalk and anterior-like cells is consistent with these cells being 'less differentiated' than prespore cells.
M
M
Krefft
L
L
Voet
J H
JH
Gregg
K L
KL
Williams
D000911:Antibodies, Monoclonal / Q000276:immunology*; D002454:Cell Differentiation; D002469:Cell Separation; D004023:Dictyostelium / Q000166:cytology / Q000254:growth & development* / Q000276:immunology; D000939:Epitopes / Q000276:immunology*; D005434:Flow Cytometry
D000911:Antibodies, Monoclonal; D000939:Epitopes