Triple immunological staining with colloidal gold, fluorescein and rhodamine as labels.
Colloidal gold particles are detectable by light microscopy with polarized light in the same epi-illumination system as for fluorescence microscopy. Colloidal gold particles can thus be used in combination with a fluorochrome for the combined immunological detection of surface membrane and cytoplasmic markers. We analyzed human bone marrow cells by a triple immunological staining for 3 different markers, using colloidal gold, fluorescein and rhodamine as labels. Our results demonstrate that such a triple immunological staining provides a powerful tool for study of the heterogeneity of small cell populations.
J J
JJ
Van Dongen
H
H
Hooijkaas
W M
WM
Comans-Bitter
K
K
Benne
T M
TM
Van Os
J
J
De Josselin de Jong
D000328:Adult; D001854:Bone Marrow Cells; D002648:Child; D019793:Fluorescein; D005452:Fluoresceins; D006046:Gold; D006801:Humans; D007158:Immunologic Techniques; D008856:Microscopy, Fluorescence / Q000379:methods; D012235:Rhodamines; D013194:Staining and Labeling; D014966:Xanthenes
D005452:Fluoresceins; D012235:Rhodamines; D014966:Xanthenes; D006046:Gold; D019793:Fluorescein