Addition of serum to electroporated cells enhances survival and transfection efficiency.
Optimal electroporation efficiency of many cell types is associated with poor survival. We show that serum rapidly reseals the membranes of electroporated cells and that timely addition of serum following electroporation can improve cell survival and transfection efficiency.
Bahnson
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Department of Radiation Oncology, University of Pittsburgh School of Medicine, PA 15261.
Boggs
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D000818:Animals; D001769:Blood; D002460:Cell Line; D002463:Cell Membrane Permeability; D002470:Cell Survival; D003470:Culture Media; D004558:Electric Stimulation; D005822:Genetic Vectors; D007700:Kinetics; D051379:Mice; D014162:Transfection
D003470:Culture Media