alpha-Dihydrodecaprenyl phosphate as a sugar carrier in the membrane of AH 70Btc hepatoma cells.
The effect of alpha-dihydrodecaprenyl phosphate, dolichyl phosphate and solanesyl phosphate on the lipid intermediate pathway for protein glycosylation was studied with crude membrane fraction prepared from AH 70Btc hepatoma cells. alpha-Dihydrodecaprenyl phosphate increased the incorporations of [14C]mannose from GDP-[14C]mannose into CHCl3-CH3OH (2:1, v/v) extract, oligosaccharide-lipid and proteins. The above and the other data showed that alpha-dihydrodecaprenyl phosphate may function as a mannose carrier in the lipid intermediate pathway.
Y
Y
Okamoto
K
K
Murakami
Y
Y
Tahara
H
H
Fukawa
N
N
Akamatsu
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004288:Dolichol Phosphates / Q000494:pharmacology; D006023:Glycoproteins / Q000096:biosynthesis; D006155:Guanosine Diphosphate Mannose / Q000378:metabolism; D007700:Kinetics; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D011106:Polyisoprenyl Phosphates / Q000494:pharmacology*; D051381:Rats; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000378:metabolism
D004288:Dolichol Phosphates; D006023:Glycoproteins; D008565:Membrane Proteins; D011106:Polyisoprenyl Phosphates; D006155:Guanosine Diphosphate Mannose; D014537:Uridine Diphosphate N-Acetylglucosamine; C048154:solanesyl phosphate; C048155:dihydrodecaprenyl phosphate