Regulation of the IgM and IgA anti-dextran B1355S response: synergy between IFN-gamma, BCGF II, and IL 2.
T cell help is required for the induction of the humoral antibody response to dextran B1355S, a type II thymus-independent bacterial polysaccharide antigen. In the present study we have identified three B cell growth and differentiation factors that can substitute for T cells in the induction of IgM and IgA antibody responses to alpha(1,3) glucan determinants on dextran B1355S. Dextran B1355S stimulated murine B cell cultures supplemented with a combination of murine recombinant interferon-gamma (IFN-gamma) and a late-acting B cell growth and differentiation factor, BCGF II, produced both IgA and IgM anti-alpha(1,3) dextran plaque-forming cells (PFC). Interleukin 2 (IL 2) was not required for those responses. In contrast, recombinant IFN-gamma and recombinant IL 2 in combination supported the induction of IgA but not IgM anti-alpha(1,3) dextran PFC. In all cases, depletion of surface IgA-bearing B cells significantly decreased IgA but not IgM anti-dextran responses, indicating that the B cells responding to those lymphokines already were committed to IgA expression. These studies indicate that B cell growth and differentiation factors can exhibit differential effects on the induction of IgA compared with IgM responses.
P D
PD
Murray
S L
SL
Swain
M P
MP
Kagnoff
D000818:Animals; D001402:B-Lymphocytes / Q000145:classification / Q000378:metabolism; D003911:Dextrans / Q000276:immunology* / Q000494:pharmacology; D004357:Drug Synergism; D006133:Growth Substances / Q000494:pharmacology*; D006462:Hemolytic Plaque Technique; D007070:Immunoglobulin A / Q000096:biosynthesis*; D007075:Immunoglobulin M / Q000096:biosynthesis*; D007371:Interferon-gamma / Q000494:pharmacology*; D007376:Interleukin-2 / Q000502:physiology*; D015847:Interleukin-4; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects; D008222:Lymphokines / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism
D003911:Dextrans; D006133:Growth Substances; D007070:Immunoglobulin A; D007075:Immunoglobulin M; D007376:Interleukin-2; D008222:Lymphokines; D011947:Receptors, Antigen, B-Cell; D015847:Interleukin-4; D007371:Interferon-gamma