Relation between Ca2+ uptake and ATPase activity in the particulate fractions of the eggshell gland mucosa of the domestic fowl and duck.
The relation between the rates of ATP-dependent Ca2+ uptake and ATP hydrolysis was studied in homogenates of eggshell gland mucosa and its subcellular fractions from the domestic fowl and duck. The Ca2+-Mg2+-ATPase activity was 5-10% of that of the "basal" Mg2+-ATPase at an optimal Ca2+ concentration in the subfractions. The presence of K+ and/or Na+ increased the rate of Ca2+ uptake and the Ca2+-Mg2+-ATPase activity; the effects of K+-Na+ were not inhibited by ouabain. The Ca/P ratio varied with the experimental conditions. At 10(-4) M Ca2+ and in the absence of K+ Na+ it was 0.8, and in their presence 0.4.
Lundholm
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D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D001693:Biological Transport, Active; D017301:Ca(2+) Mg(2+)-ATPase; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002645:Chickens; D004372:Ducks; D004528:Egg Shell; D005088:Exocrine Glands / Q000378:metabolism*; D005260:Female; D007700:Kinetics; D009092:Mucous Membrane / Q000378:metabolism; D012430:Ruthenium Red / Q000494:pharmacology; D013045:Species Specificity; D014268:Trifluoperazine / Q000494:pharmacology
D012430:Ruthenium Red; D014268:Trifluoperazine; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases; D002118:Calcium