Measurement of dimethylglycine in biological fluids.
The method of quantitating N,N-dimethylglycine involves cation-exchange high-performance liquid chromatography and detection of dimethylglycine with dimethylglycine dehydrogenase. Dimethylglycine was added to plasma and urine and samples were assayed for dimethylglycine. Plasma and urine to which no dimethylglycine was added were also assayed. Recoveries of added dimethylglycine were 99 to 104% with no endogenous dimethylglycine found in rat plasma or normal human urine. The human plasma used contained a small amount of endogenous dimethylglycine. The cation-exchange chromatography separates dimethylglycine from other compounds which can serve as substrates for dimethylglycine dehydrogenase. Repeatability of the assay is +/- 10%. Using this method we have identified dimethylglycine in the urine of a 1-month-old female human patient.
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D002250:Carbon Radioisotopes; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D050893:Dimethylglycine Dehydrogenase; D005998:Glycine / Q000031:analogs & derivatives*; D006801:Humans; D024101:Mitochondrial Proteins; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism; D011865:Radioisotope Dilution Technique; D012521:Sarcosine / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D013379:Substrate Specificity
D002250:Carbon Radioisotopes; D024101:Mitochondrial Proteins; C025138:dimethylglycine; D010089:Oxidoreductases, N-Demethylating; C494398:DMGDH protein, human; D050893:Dimethylglycine Dehydrogenase; D005998:Glycine; D012521:Sarcosine