[Fluid storage of thrombocytes. In vitro viability of thrombocytes at 12 degrees C storage temperature].
Platelet concentrates from ACD-blood were stored with and without agitation at +12 degrees C for 3 days. pH-values, hypotonic shock response, serotonin uptake and adenine nucleotides were investigated. In addition platelet shape was morphologically differentiated in discs with and without pseudopods, in spheres and irregular platelets. In accordance with platelet storage at +22 degrees C an optimum platelet number and gentle agitation were essential to maintain in vitro vitality at +12 degrees C for 3 days. The number of discoid platelets declined from 76% in fresh platelet concentrates to 25% after 3 days at +12 degrees C although pH-values did not fall below 6.8 in the agitated concentrates. A diminished post-transfusion survival could result from this, unless the shape change reverses in the circulation.
Berndsen
G
G
D000227:Adenine Nucleotides / Q000097:blood; D001792:Blood Platelets / Q000378:metabolism / Q000502:physiology*; D001793:Blood Preservation / Q000379:methods; D002470:Cell Survival; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D012701:Serotonin / Q000378:metabolism; D013696:Temperature
D000227:Adenine Nucleotides; D012701:Serotonin
Zur Thrombozytenflüssiglagerung In-Vitro-Vitalität von Thrombozyten bei +12 grade C Lagerungstemperatur.