The amino acid sequence of the acidic subunit B-chain of crotoxin.
The B-chain of the acidic subunit of crotoxin proved refractory to Edman degradation. When subjected to sequence analysis using tandem mass spectrometry, pyroglutamate was found at the amino-terminal end, even though earlier attempts to de-block with pyroglutamate aminopeptidase were unsuccessful. The B-chain contained 35 amino acids and showed 91% amino acid identity with the corresponding segment from Mojave toxin, a homologous neurotoxin from Crotalus scutulatus scutulatus. The sequence of the last 24 residues of the B-chain is consistent with that previously published (Aird, S.D., Kaiser, I.I., Lewis, R.V. and Kruggel, W.G. (1985) Biochemistry 24, 7054-7058), except at position 20, where Edman degradation gave glycine and mass spectrometry gave glutamic acid.
Aird
S D
SD
Department of Molecular Biology, University of Wyoming, Laramie 82071.
Yates
J R
JR
Martino
P A
PA
Shabanowitz
J
J
Hunt
D F
DF
Kaiser
I I
II
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003435:Crotalid Venoms; D003439:Crotoxin; D005583:Fourier Analysis; D046911:Macromolecular Substances; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification; D010741:Phospholipases A; D012689:Sequence Homology, Nucleic Acid; D012911:Snakes
D003435:Crotalid Venoms; D046911:Macromolecular Substances; D010446:Peptide Fragments; D003439:Crotoxin; D010741:Phospholipases A