Characteristics of the binding of aminoglycoside antibiotics to teichoic acids. A potential model system for interaction of aminoglycosides with polyanions.
The binding of the aminoglycoside antibiotic dihydrostreptomycin to defined cell-wall teichoic acids and to lipoteichoic acid isolated from various gram-positive eubacteria was followed by equilibrium dialysis. Dihydrostreptomycin was used at a wide range of concentration under different conditions of ionic strength, concentration of teichoic acid, presence of cationic molecules like Mg2+, spermidine, other aminoglycoside antibiotics (gentamicin, neomycin, paromomycin). Interaction of dihydrostreptomycin with teichoic acid was found to be a cooperative binding process. The binding characteristics seem to be dependent on structural features of teichoic acid and are influenced by cationic molecules. Mg2+, spermidine and other aminoglycosides antibiotics inhibit the binding of dihydrostreptomycin to teichoic acid competitively. The binding of aminoglycosides to teichoic acids is considered as a model system for the interaction of aminoglycoside antibiotics with cellular polyanions. Conclusions of physiological significance are drawn.
Kusser
W
W
Zimmer
K
K
Fiedler
F
F
D000617:Aminoglycosides / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001667:Binding, Competitive; D002412:Cations; D002473:Cell Wall / Q000378:metabolism; D003956:Dialysis / Q000379:methods; D004096:Dihydrostreptomycin Sulfate / Q000378:metabolism; D000071228:Polyelectrolytes; D011108:Polymers / Q000378:metabolism*; D013045:Species Specificity; D013305:Streptomyces griseus; D013682:Teichoic Acids / Q000378:metabolism*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D002412:Cations; D004096:Dihydrostreptomycin Sulfate; D000071228:Polyelectrolytes; D011108:Polymers; D013682:Teichoic Acids