Structure and biological activity of polysomes stained with Coomassie blue.
Polysomes prestained with Coomassie blue were fractionated on sucrose density gradients giving rise to visible bands corresponding to different size classes of aggregates. Coomassie blue staining enhanced the capacities of brain and liver polysomes to synthesize proteins in vitro, including the synthesis of neuron-specific enolase. This positive action of the dye was restricted to polysomes and was not manifested when mRNAs isolated from prestained polysomes were tested in in vitro translation or reverse transcription, indicating that the action of the dye consists in stabilization of polysomal structure.
M R
MR
Murthy
N
N
Hanna
A D
AD
Bharucha
R
R
Charbonneau
J L
JL
Viallard
B
B
Dastugue
D000818:Animals; D002499:Centrifugation, Density Gradient; D066298:In Vitro Techniques; D008297:Male; D010751:Phosphopyruvate Hydratase / Q000096:biosynthesis; D011132:Polyribosomes / Q000032:analysis* / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D012394:Rosaniline Dyes; D013194:Staining and Labeling
D012333:RNA, Messenger; D012394:Rosaniline Dyes; D010751:Phosphopyruvate Hydratase; C004692:coomassie Brilliant Blue