Retinal S-antigen epitopes in vertebrate and invertebrate photoreceptors.
Monoclonal antibodies specific for the retinal S-antigen were obtained by hybridization of spleen cells from a BALB/c mouse immunized with bovine S-antigen and NS-1 myeloma cells. Five selected antibodies specifically labeled the photoreceptor cells of the retina by immunofluorescence. Whereas antibody S9E2 only reacted with bovine S-antigen, the other antibodies showed interspecies cross-reactivity. They were used for the characterization of specific epitopes of S-antigen in photoreceptors from a wide range of species representative of various classes of vertebrates and invertebrates. The presence of S-antigen in distant species (vertebrates, Amphioxus, nemerteans, annelids, molluscs) indicates a high phylogenetic stability and suggests an important role for this protein in photoreceptor function.
Mirshahi
M
M
Boucheix
C
C
Collenot
G
G
Thillaye
B
B
Faure
J P
JP
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D000918:Antibody Specificity; D000941:Antigens / Q000032:analysis*; D019393:Arrestin; D000939:Epitopes / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007448:Invertebrates; D010786:Photoreceptor Cells / Q000276:immunology*; D013045:Species Specificity; D014714:Vertebrates
D000911:Antibodies, Monoclonal; D000941:Antigens; D019393:Arrestin; D000939:Epitopes