Carbohydrate determinants associated with carcinoembryonic antigen (CEA).
The reactivities of eight purified preparations of carcinoembryonic antigen with monoclonal antibodies directed to tumor-associated carbohydrate determinants have been studied. All eight preparations showed strong reactivities with AH6, which defines Y structure (Fuc alpha 1----2Gal beta 1----4[Fuc alpha 1----3] GlcNAc beta 1----R), whereas only a few preparations showed reactivity with FH4-defining dimeric X determinants, (Gal beta 1----4 [Fuc alpha 1----3]GlcNAc beta 1----3Gal beta 1----4 [Fuc alpha 1----3]GlcNA beta 1----3Gal beta 1----R). No other antibodies tested showed any reactivity with these preparations. These carbohydrate markers associated with carcinoembryonic antigen will be useful to enhance the diagnostic value of the antigen.
E J
EJ
Nichols
R
R
Kannagi
S I
SI
Hakomori
M J
MJ
Krantz
A
A
Fuks
D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000276:immunology; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D000939:Epitopes; D006801:Humans
D002241:Carbohydrates; D002272:Carcinoembryonic Antigen; D000939:Epitopes