Purification of a glycoprotein excreted by Trypanosoma cruzi to increase the permeability of the host-cell membrane.
During invasion of the prospective host cell, metacyclic forms of Trypanosoma cruzi render the membrane of HeLa cells permeable to the alpha-sarcin toxin, by excreting a glycoprotein with N-acetyl-D-glucosamine residues. The molecular weight of the glycoprotein is 64,000 dalton and its isoelectric point is 4.8.
Department of Parasitology, Faculty of Science, University of Granada, Spain.
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S
Castanys
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F
Gamarro
L M
LM
Ruiz-Pérez
A
A
Osuna
D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects; D004722:Endoribonucleases; D005656:Fungal Proteins / Q000633:toxicity; D006023:Glycoproteins / Q000302:isolation & purification* / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D014349:Trypanosoma cruzi / Q000032:analysis*
D005656:Fungal Proteins; D006023:Glycoproteins; C014801:alpha-sarcin; D004722:Endoribonucleases