Experimental model of disc herniations in rats for study of nucleolytic drugs.
An experimental model of disc herniation in tail discs of rats is described. Constant result on nucleus hernia and intervertebral narrowing were obtained by an easy manipulation on numerous rats. Intradiscal injection of aprotinin produced a widening of the disc height. Trypsin, collagenase, chymopapain, and hyaluronidase induced a narrowing of disc height; trypsin induced macroscopic necrosis of the soft surrounding tissues; and collagenase had a destructive effect on nucleus pulposus, annulus fibrosus, and even on end-plates. Chymopapain and hyaluronidase acted mainly on nucleus pulposus. Hyaluronidase could be of interest as a nucleolytic drug and needs further studies on optimal dosage and lack of side effects in the surrounding tissues before injecting it into human discs.
D000818:Animals; D007611:Aprotinin / Q000627:therapeutic use; D002917:Chymopapain / Q000627:therapeutic use; D004195:Disease Models, Animal; D006821:Hyaluronoglucosaminidase / Q000627:therapeutic use; D006867:Hydrolases / Q000627:therapeutic use*; D007404:Intervertebral Disc Chemolysis; D007405:Intervertebral Disc Displacement / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D003012:Microbial Collagenase / Q000627:therapeutic use; D051381:Rats; D011917:Rats, Inbred Lew; D014357:Trypsin / Q000627:therapeutic use
D007611:Aprotinin; D006867:Hydrolases; D006821:Hyaluronoglucosaminidase; D014357:Trypsin; D002917:Chymopapain; D003012:Microbial Collagenase