N-alkanes induce the synthesis of cytochrome P-450 mRNA in Candida maltosa.
In the yeast Candida maltosa the level of cytochrome P-450 was 100-300-fold higher in alkane-grown cells than in glucose-grown ones (detected by a radioimmunoassay). It was shown immunochemically (1) that this was not the result of an assembly of preexisting apoenzyme with the prosthetic heme group. By cell-free translation of total poly(A)RNA in a wheat germ system and subsequent immunoprecipitation it was shown that the amount of mRNA coding for cytochrome P-450 paralleled its concentration in the cell.
D000473:Alkanes / Q000494:pharmacology*; D002175:Candida / Q000201:enzymology / Q000235:genetics*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D005947:Glucose / Q000494:pharmacology; D011061:Poly A / Q000378:metabolism; D014176:Protein Biosynthesis; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D011863:Radioimmunoassay
D000473:Alkanes; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D003577:Cytochrome P-450 Enzyme System; D005947:Glucose