Enumeration of lymphocyte subpopulations by immunofluorescent staining of whole blood smears.
A simple and rapid technique for the enumeration of lymphocyte subpopulations by immunofluorescent staining of blood smears is described. The extremely small volumes of blood required make the technique particularly suitable for samples from paediatric patients. Results compare closely with conventional staining of lymphocytes in suspension.
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006801:Humans; D007231:Infant, Newborn; D008214:Lymphocytes / Q000145:classification* / Q000166:cytology; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface