Type II collagen arthritis: identification of arthritogenic epitopes using fractionated anticollagen IgG.
Affinity-purified anticollagen IgG was fractionated on purified cyanogen bromide-derived collagen peptide Sepharose. The antibody fraction bound to the peptides was eluted and tested for its ability to induce passive arthritis in recipients. Anticollagen IgG bound to peptide 5 (alpha 1(II)-CB8-10 and alpha 1(II)CB11-8) and to peptide 6 (alpha 1(II)CB11) were active in inducing passive arthritis. Other peptide bound fractions were inactive. These observations suggest that the arthritogenic domain in Type II collagen is restricted to alpha 1(II)CB11.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000906:Antibodies / Q000008:administration & dosage / Q000276:immunology / Q000302:isolation & purification; D001168:Arthritis / Q000276:immunology*; D001169:Arthritis, Experimental / Q000139:chemically induced / Q000276:immunology*; D002356:Cartilage / Q000276:immunology; D002846:Chromatography, Affinity / Q000379:methods; D003094:Collagen / Q000276:immunology*; D003488:Cyanogen Bromide / Q000494:pharmacology; D000939:Epitopes / Q000032:analysis* / Q000276:immunology; D005455:Fluorescent Antibody Technique; D007074:Immunoglobulin G / Q000008:administration & dosage; D008297:Male; D010455:Peptides / Q000276:immunology / Q000302:isolation & purification; D011487:Protein Conformation; D051381:Rats; D011919:Rats, Inbred Strains
D000596:Amino Acids; D000906:Antibodies; D000939:Epitopes; D007074:Immunoglobulin G; D010455:Peptides; D003094:Collagen; D003488:Cyanogen Bromide