A single bone marrow-derived stromal cell type supports the in vitro growth of early lymphoid and myeloid cells.
A clonal cell line (ALC) derived from murine bone marrow stroma is capable of supporting the continuous, in vitro growth of early lymphoid and myeloid cell populations. The growth-promoting effects of ALC are in part mediated through M-CSF and a pre-B cell growth factor, both of which accumulate in ALC-culture supernatant. To analyze the lymphoid growth factor produced by ALC cells, we derived a pre-B cell indicator line that is dependent on ALC-growth-conditioned medium. Using a combination of biological and biochemical analyses, we have established that the pre-B cell growth factor produced by ALC cells is distinct from IL-1, IL-2, IL-3, and IL-4 (BSF-1), suggesting that the early stages of B-cell development are regulated by a unique stroma-derived growth factor.
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology*; D001854:Bone Marrow Cells; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002999:Clone Cells; D003094:Collagen / Q000096:biosynthesis; D003470:Culture Media; D004247:DNA / Q000032:analysis; D006133:Growth Substances / Q000494:pharmacology; D006854:Hydrocortisone / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D012313:RNA / Q000032:analysis; D011994:Recombinant Proteins / Q000494:pharmacology
D003470:Culture Media; D006133:Growth Substances; D011994:Recombinant Proteins; D012313:RNA; D003094:Collagen; D004247:DNA; D006854:Hydrocortisone