title: [Purification of a soluble tetrodotoxin-sensitive protein from the cytoplasmic fraction of bovine brain tissue].
abstract: Highly purified protein inducing tetrodotoxin-dependent Na fluxes in liposomal membrane was obtained from the cytoplasmic fraction of the bovine brain. The protein was purified by anion-exchange chromatography on DEAE-Servacell and wheat germ agglutinin sepharose (WGA) followed by gel filtration on sepharose 4B. It is a high-molecular weight acidic glycoprotein; during denaturation under reducing conditions it forms 55 kD subunits. It is suggested that the tetrodotoxin-sensitive protein could be a soluble intracellular precursor of the voltage-dependent sodium channels.
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects; D001923:Brain Chemistry; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D006023:Glycoproteins / Q000302:isolation & purification*; D007473:Ion Channels / Q000032:analysis* / Q000187:drug effects; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D012964:Sodium / Q000378:metabolism*; D013779:Tetrodotoxin / Q000494:pharmacology*
chemical_list: D006023:Glycoproteins; D007473:Ion Channels; D009419:Nerve Tissue Proteins; D013779:Tetrodotoxin; D012964:Sodium
vernacular_title: Ochistka rastvorimogo tetrodotoksinchuvstvitel'nogo belka iz tsitoplazmicheskoĭ fraktsii tkani golovnogo mozga krupnogo rogatogo skota.