title: The outgrowth of parietal endoderm from mouse teratocarcinoma stem-cell embryoid bodies.
abstract: Teratocarcinoma stem cells can be used to study certain events occurring during early mouse embryogenesis. We report that the outgrowth of parietal endoderm from teratocarcinoma stem-cell embryoid bodies in vitro is analogous to the same process in vivo in terms of the spatial distribution of endoderm types: only parietal endoderm migrates away from the aggregate, whereas visceral endoderm remains associated with the embryoid body. The outgrowths generated on a substrate of type-I collagen from PSA-1 and retinoic-acid-treated F 9 embryoid bodies were found to be comparable, even though these aggregates express different endoderm types. We demonstrated that retinoic-acid-treated F 9 embryoid bodies that contain essentially only visceral endoderm in suspension culture can nonetheless generate parietal-endoderm outgrowth when plated on type-I collagen, suggesting that substrate interaction plays an important role in inducing parietal-endoderm differentiation. These data indicate the usefulness and relevance of studying endoderm differentiation and outgrowth in vitro employing the teratocarcinoma model system.
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D003094:Collagen / Q000378:metabolism; D054278:Embryonal Carcinoma Stem Cells; D004707:Endoderm / Q000032:analysis / Q000166:cytology* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006017:Glycolipids / Q000032:analysis; D007797:Laminin / Q000032:analysis; D016256:Lewis X Antigen; D051379:Mice; D014411:Neoplastic Stem Cells / Q000032:analysis / Q000166:cytology* / Q000378:metabolism; D013724:Teratoma; D000509:alpha-Fetoproteins / Q000032:analysis
chemical_list: D006017:Glycolipids; D007797:Laminin; D016256:Lewis X Antigen; D000509:alpha-Fetoproteins; D003094:Collagen