15-HETE "modulates" expression of C3b receptor (CR1) antigen on peripheral blood B-lymphocytes.
We have studied the effect of the lipoxygenase metabolite, 15-HETE, on the expression of the human C3b receptor (CR1) by a B-lymphocyte enriched population of human peripheral blood leukocytes. The number of CR1 antigenic sites expressed by B-lymphocytes isolated from HLA typed donors was determined by equilibrium binding studies using an 125 I-labelled mouse monoclonal anti CR1 antibody before and after 16 hrs incubation in RPMI alone or containing 10(-6)M, 10(-7)M or 10(-8)M final concentration of 15-HETE. In B44- subjects CR1 expression on B cells increased 63% after incubation in RPMI alone. This increase was inhibited in the presence of 10(-6)M and 10(-7)M 15-HETE (23% and 30% increase respectively). In contrast, B44+ individuals showed a smaller increase in CR1 numbers when incubated in RPMI alone. In the presence of 15-HETE CR1 antigenic sites continued to increase. When B44+ subjects were classified as A29+ or A29-, donors that were A29+ B44+ accounted for the augmentation observed while A29- B44+ individuals did not differ from individuals that were A29- B44-.
D000954:Antigens, Surface / Q000032:analysis; D001095:Arachidonic Acids / Q000276:immunology / Q000494:pharmacology; D001402:B-Lymphocytes / Q000276:immunology / Q000648:ultrastructure*; D001665:Binding Sites; D000939:Epitopes / Q000032:analysis*; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000276:immunology* / Q000494:pharmacology; D011951:Receptors, Complement / Q000187:drug effects* / Q000276:immunology; D017463:Receptors, Complement 3b
D000954:Antigens, Surface; D001095:Arachidonic Acids; D000939:Epitopes; D006680:HLA Antigens; D006893:Hydroxyeicosatetraenoic Acids; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid