Unmasking antigen determinants in amyloid.
Rehydrated paraffin sections of formalin-fixed, amyloid-containing tissues were treated with denaturing agents (guanidine and urea) and reducing agents (DDT and mercaptoethanol) before immunostaining, in an attempt to expose antigenic determinants hidden in the rigid structure of amyloid fibrils. Pre-treatment overnight with 6 M guanidine or urea was found beneficial, especially in specimens from familial amyloid polyneuropathy of the Portuguese type. Addition of reducing agents had no major effect. Modifications of this method may be useful in unmasking other antigens that are polymerized or considered destroyed by fixation and paraffin embedding.
D000682:Amyloid / Q000276:immunology*; D000911:Antibodies, Monoclonal; D003634:DDT; D000939:Epitopes / Q000032:analysis*; D019791:Guanidine; D006146:Guanidines; D006651:Histocytochemistry; D006801:Humans; D007668:Kidney / Q000166:cytology; D008623:Mercaptoethanol; D008722:Methods; D011228:Prealbumin / Q000032:analysis / Q000276:immunology; D014508:Urea
D000682:Amyloid; D000911:Antibodies, Monoclonal; D000939:Epitopes; D006146:Guanidines; D011228:Prealbumin; D008623:Mercaptoethanol; D014508:Urea; D003634:DDT; D019791:Guanidine