Experimental study on size-limitation of myocardial infarct in swine produced by a coronary balloon-catheterization. I. Preliminary report; morphometry of infarct size.
Experimental myocardial infarction was produced in 15 subject swine using a balloon catheter to occlude the left anterior descending coronary artery (LAD). After 30 minutes obstruction, the LAD was reperfused, and then the animals were sacrificed at 3 hours, 6 hours, 24 hours, and 48 hours after the onset of coronary occlusion, respectively. During and after the experiment they were investigated physiologically and pathomorphologically to measure the size of myocardial infarct, and the obtained results are as follows; Experimentally produced myocardial infarcts could be detected histopathologically by routine gross and light-microscopical means in the group which had suffered from ischemia for over 24 hours till sacrifice. Ischemic areas could not be distinguished from normal areas of the myocardium by routine techniques, but could be identified enzyme-histochemically in the groups observed for less than 6 hours. The mean weight ratio of infarcted myocardium to total ventricular myocardium was 25 +/- 3.6%. This method was useful for measuring infarct size.
D000818:Animals; D001391:Azo Compounds; D006328:Cardiac Catheterization / Q000379:methods / Q000401:mortality; D004195:Disease Models, Animal; D004562:Electrocardiography; D005260:Female; D006416:Hematoxylin; D008297:Male; D009203:Myocardial Infarction / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D009929:Organ Size; D013194:Staining and Labeling; D013552:Swine; D013997:Time Factors
D001391:Azo Compounds; C011049:oil red O; D006416:Hematoxylin