Control of PTH secretion is mediated through calcium channels and is blocked by pertussis toxin treatment of parathyroid cells.
Parathyroid hormone secretion is negatively regulated by calcium. We utilized calcium channel agents: +202-791, a calcium channel agonist and -202-791, a calcium channel antagonist, to evaluate the role of calcium channels in PTH secretion. +202-791 inhibited PTH release from bovine parathyroid cells and the antagonist stimulated release. Incubation with pertussis toxin which ADP-ribosylates and inactivates a guanine nucleotide regulatory protein (G-protein) releases the inhibition by the calcium channel agonist. These findings indicate that a G-protein is interposed between the calcium channel and a putative intracellular site controlling PTH secretion.
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D009539:Nicotinic Acids / Q000494:pharmacology; D010069:Oxadiazoles; D010280:Parathyroid Glands / Q000187:drug effects / Q000378:metabolism*; D010281:Parathyroid Hormone / Q000378:metabolism*; D037342:Pertussis Toxin; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D007473:Ion Channels; D009539:Nicotinic Acids; D010069:Oxadiazoles; D010281:Parathyroid Hormone; D010566:Virulence Factors, Bordetella; C049834:PN 202-791; D037342:Pertussis Toxin; D002118:Calcium