Cytoskeletal alterations leading to Mallory body formation in livers of mice fed 3,5-diethoxycarbonyl-1,4-dihydrocollidine.
Cytoskeletal alterations of hepatocytes were studied in mice fed 3,5-diethoxycarbonyl-1,4-dihydrocollidine from the onset of the experiment to the time when Mallory bodies were induced, using immunofluorescent microscopy and a method which we devised in order to visualize the cytoskeletal framework of hepatocytes. The results showed that the cytoskeletal framework of the normal murine hepatocyte is composed of a network of uniformly distributed, interconnecting bundles of filaments. In the drug-fed mice, the cytoskeletal network of the hepatocytes became coarser and distorted. The Mallory body-containing hepatocytes were mostly devoid of cytoskeletal filaments, containing only a few thick bundles of filaments, which appeared partly smudgy and electron-dense. These results suggest that the formation of Mallory bodies in this animal model is associated with a marked derangement of the cytoskeletal framework, which appears to result from the progressive loss of normal filaments and the aggregation of abnormal ones.
D000818:Animals; D003599:Cytoskeleton / Q000187:drug effects* / Q000648:ultrastructure; D003997:Dicarbethoxydihydrocollidine / Q000494:pharmacology*; D004095:Dihydropyridines / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D007382:Intermediate Filaments / Q000648:ultrastructure; D007633:Keratins / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008854:Microscopy, Electron / Q000379:methods
D004095:Dihydropyridines; D003997:Dicarbethoxydihydrocollidine; D007633:Keratins