title: The amino acid sequence of Limulus C-reactive protein. Evidence of polymorphism.
abstract: The amino acid sequence, the positions of the disulfide bonds, and the site of glycosylation for the three subunits of Limulus C-reactive proteins (CRPs) 1.1, 1.4, and 3.3 have been established. The three subunits were shown to exist approximately in equimolar amount and are tightly associated. The hexagonal structure of Limulus CRP, as revealed by electron microscopic studies of Fernandez-Moran et al. (Fernandez-Moran, H., Marchalonis, J., and Edelman, G. M. (1968) J. Mol. Biol. 32, 467-469) might consist of two each of the subunits. The three subunits share an identical amino-terminal sequence of 44 residues and a carboxyl-terminal sequence from residues 206 to 218. Microheterogeneity exists to the extent of 10 to 11% for the entire protein. The positions of 6 half-cystines that form the three disulfide bonds and the site of glycosylation are constant in all subunits. Sequence analyses of peptides derived from enzymatic and chemical cleavages of affinity purified Limulus CRP indicate that subunits other than the three mentioned above exist in the hemolymph. Limulus CRP is therefore polymorphic. Topological analyses of Limulus CRPs, human CRP, rabbit CRP, human amyloid P-component, and Syrian hamster female protein indicate that the seven proteins may originate from the same ancestral gene. Using the topological data generated from the amino acid sequences of the proteins, we calculate that human and Limulus CRPs diverged about 500 million years ago. This figure is in general agreement with the evolutionary distance postulated by anthropological estimation of 400-500 million years.
mesh_terms: D000510:Alpha-Globulins; D000595:Amino Acid Sequence; D000682:Amyloid; D000818:Animals; D005075:Biological Evolution; D002097:C-Reactive Protein / Q000235:genetics; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA / Q000235:genetics; D004220:Disulfides; D005944:Glucosamine / Q000378:metabolism; D006737:Horseshoe Crabs / Q000032:analysis*; D006801:Humans; D008647:Mesocricetus; D010446:Peptide Fragments; D011110:Polymorphism, Genetic; D011817:Rabbits
chemical_list: D000510:Alpha-Globulins; D000682:Amyloid; D004220:Disulfides; D010446:Peptide Fragments; C030974:female protein, hamster; D002097:C-Reactive Protein; D004247:DNA; D005944:Glucosamine