Effects of long-chain fatty-acyl esters of coenzyme A and carnitine on cell-free rat heart preparations.
The purpose of this study was to investigate the effects of fatty acyl CoA and carnitine esters on the glycolytic system of the rat heart. Using a respiring incubation mixture containing a whole-heart homogenate it was observed that oleoyl-CoA slowed down the glucose disappearance whereas lactate accumulation did not change. Experiments were also performed by means of an incubation mixture prepared with a soluble heart extract, considered to contain all glycolytic enzymes present in heart fibres. Palmitoyl-CoA or oleoyl-CoA as well as palmitoyl carnitine, added separately or together, were unable to alter the glucose disappearance and lactate accumulation in this mixture. These data suggest that long chain acyl-esters have not direct inhibitory actions on the heart glycolytic activity. However, CoA esters seem to exert indirect inhibitory effects which may be relevant to the myocardium under oxygen restriction situations.
D000214:Acyl Coenzyme A / Q000031:analogs & derivatives / Q000494:pharmacology*; D000818:Animals; D002331:Carnitine / Q000031:analogs & derivatives / Q000494:pharmacology*; D005260:Female; D006019:Glycolysis / Q000187:drug effects*; D006321:Heart / Q000187:drug effects; D066298:In Vitro Techniques; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D010171:Palmitoyl Coenzyme A / Q000494:pharmacology; D051381:Rats; D014020:Tissue Extracts / Q000378:metabolism
D000214:Acyl Coenzyme A; D007773:Lactates; D014020:Tissue Extracts; C017585:oleoyl-coenzyme A; D010171:Palmitoyl Coenzyme A; D019344:Lactic Acid; D002331:Carnitine