Absence of deoxyuridine and 5-hydroxymethyldeoxyuridine in the DNA from three tissues of mice of various ages.
Mutational damage to DNA may modulate the aging process as well as contribute to the high incidence of cancer in older animals. Uracil (Ura) is the deamination product of cytosine and hydroxymethyluracil (HMU) is an oxidation product of thymine. Ura, when generated from cytosine, induces mutations by mispairing with adenine. Both HMU and Ura are known to be excised from DNA by glycosylases that cleave the respective N-glycosidic bonds. This hydrolysis leaves apyrimidinic sites which are subsequently repaired by excision repair. In this report a sensitive method to detect these altered bases of HPLC separation of the components of DNA hydrolysates is described. Neither deoxyuridine (dU) nor 5-hydroxymethyldeoxyuridine (dHMU) were found in hydrolysates of DNA samples from brain, liver or small intestinal mucosa of mice of different ages.
D000375:Aging; D000818:Animals; D001921:Brain / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000378:metabolism*; D003857:Deoxyuridine / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012313:RNA / Q000378:metabolism; D013936:Thymidine / Q000031:analogs & derivatives* / Q000378:metabolism
C018080:5-hydroxymethyl-2'-deoxyuridine; D012313:RNA; D004247:DNA; D013936:Thymidine; D003857:Deoxyuridine