Identification of antigenic proteins of setaria cervi by immunoblotting technique.
Identification and characterization of antigenic proteins of Setaria cervi (bovine filarial parasite) adults and microfilariae was done by immunoblotting technique using hyperimmune rabbit sera against S. cervi and Brugia malayi. The antigens recognized by these sera were detected by using 125I protein-A followed by autoradiography. Fifteen different antigens were observed to be common between adult and microfilarial stages of the parasite. Some stage specific antigens were also identified. Many antigens of S. cervi adults and microfilariae were also recognized by rabbit anti-B.malayi serum showing the existence of common antigenic determinants between the bovine and human filarial parasites.
D000818:Animals; D001345:Autoradiography; D002417:Cattle; D003101:Collodion; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis* / Q000276:immunology; D005370:Filarioidea / Q000254:growth & development / Q000276:immunology*; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D007457:Iodine Radioisotopes; D008842:Microfilariae / Q000276:immunology; D008970:Molecular Weight; D012719:Setariasis / Q000175:diagnosis / Q000276:immunology
D000939:Epitopes; D007457:Iodine Radioisotopes; D003101:Collodion