Expression of the gag-pol fusion protein of Moloney murine leukemia virus without gag protein does not induce virion formation or proteolytic processing.
A mutant of Moloney murine leukemia virus was generated in which the UAG termination codon at the 3' end of the gag gene was changed to a CAG codon encoding glutamine. Cells carrying the mutant provirus constitutively express the gag-pol fusion protein and no detectable gag protein. The precursor is stable, is not processed by the protease domain within the precursor, and does not induce assembly and release of virion particles.
D004252:DNA Mutational Analysis; D015683:Gene Products, gag; D005816:Genetic Complementation Test; D008979:Moloney murine leukemia virus / Q000502:physiology*; D011499:Protein Processing, Post-Translational; D012194:RNA-Directed DNA Polymerase / Q000235:genetics; D012191:Retroviridae Proteins / Q000235:genetics*; D013329:Structure-Activity Relationship; D014760:Viral Fusion Proteins / Q000235:genetics*; D014779:Virus Replication
D015683:Gene Products, gag; D012191:Retroviridae Proteins; D014760:Viral Fusion Proteins; D012194:RNA-Directed DNA Polymerase