title: Antigen specific human T cell lines specific for cobalt chloride.
abstract: T lymphocyte lines specific for cobalt (CoCl2) were established from one cobalt and nickel sensitive donor. The lines were generated by stimulating peripheral blood mononuclear cells (PBL) with CoCl2 for 7 days. The activated blasts were maintained in interleukin 2 containing media and every 7-10 day restimulated with antigen (CoCl2) and fresh irradiated PBL. The antigen specificity of the lines was attested by their capacity to proliferate and release of IL 2 under restimulation by CoCl2 and not by an antigen like NiSO4, towards which the donor was also sensitized. The cell lines were of the helper phenotype, T3/T4 positive subset, as determined by monoclonal antibodies. Analysis of the HLA class II restriction by using allogeneic PBL as antigen presenting cells and the capacity of anti HLA-D antibodies to effectively inhibit the specific response indicated that the antigen specific T cell response to contact allergens like cobalt chloride is restricted to HLA class II antigens.
mesh_terms: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000276:immunology; D002455:Cell Division; D002460:Cell Line; D003035:Cobalt / Q000276:immunology*; D003877:Dermatitis, Contact / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000276:immunology; D009532:Nickel / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000939:Epitopes; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D003035:Cobalt; C029938:nickel sulfate; D009532:Nickel; C018021:cobaltous chloride