Myelination by transplanted fetal and neonatal oligodendrocytes in a dysmyelinating mutant.
Demyelination is a major feature of CNS injury and disease, including multiple sclerosis. To examine the potential for myelination by transplanted oligodendrocytes, initially described by Gumpel et al., we have transplanted neonatal cortex of mice with normal myelin into a dysmyelinating mutant, the shiverer mouse. We have found that oligodendrocyte precursors mature and synthesize myelin following transplantation. Immunostaining with antibodies to myelin basic protein (MBP), neurofilament protein and glial fibrillary acidic protein, demonstrates myelination both within the graft and extending out into the host, axonal sprouting from the graft which parallels the MBP-reactivity, and minimal astrocytic proliferation in response to the transplant.
D000818:Animals; D000831:Animals, Newborn; D003711:Demyelinating Diseases / Q000378:metabolism / Q000628:therapy*; D005333:Fetus; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007381:Intermediate Filament Proteins / Q000378:metabolism; D051379:Mice; D008818:Mice, Neurologic Mutants; D004676:Myelin Basic Protein / Q000378:metabolism; D009186:Myelin Sheath / Q000502:physiology*; D016900:Neurofilament Proteins; D009457:Neuroglia / Q000637:transplantation*; D009836:Oligodendroglia / Q000378:metabolism / Q000637:transplantation*
D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D004676:Myelin Basic Protein; D016900:Neurofilament Proteins