Nuclear actin and transport of RNA.
The role of nuclear actin filaments in the RNA transport was investigated. Mouse lymphoma cells, L5178Y, were labeled for 20 min with 3H-uridine, and the isolated nuclei were incubated in a medium consisting of 0.25 M sucrose, 10 mM Tris-HCl (pH 7.5), 2 mM CaCl2, 1 mM ATP and 1mM PMSF. Release of the rapidly labeled RNA from the nuclei was temperature-dependent and was stimulated by ATP. Phalloidin, an inhibitor of actin filament depolymerization, had no effect on the system at 10 or 100 micrograms/ml. Therefore, actin filament depolymerization may not be involved in the transport of RNA.
D000199:Actins / Q000502:physiology*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002467:Cell Nucleus / Q000378:metabolism*; D007700:Kinetics; D007940:Leukemia L5178 / Q000378:metabolism; D051379:Mice; D010590:Phalloidine / Q000494:pharmacology; D012313:RNA / Q000378:metabolism / Q000493:pharmacokinetics*; D014407:Tumor Cells, Cultured
D000199:Actins; D010590:Phalloidine; D012313:RNA