Increased content of mRNA for a precursor of S-adenosylmethionine decarboxylase in rat prostate after treatment with 2-difluoromethylornithine.
Total poly(A)-containing mRNA was isolated from rat ventral prostate and translated in a reticulocyte lysate system. The proteins corresponding to S-adenosylmethionine decarboxylase were precipitated with a specific antiserum to this protein. Two proteins were found; one having an Mr of 32,000, which corresponded to the subunit of this enzyme, and a larger protein of Mr 37,000. Immunopurification of polysomes with the antiserum to S-adenosylmethionine decarboxylase followed by mRNA extraction yielded an mRNA preparation which was 10-30% pure mRNA for S-adenosylmethionine decarboxylase. The translation of this mRNA showed clearly that the protein of Mr 37,000 was a precursor of the Mr 32,000 S-adenosylmethionine decarboxylase subunit. Treatment with 2-difluoromethylornithine, which depletes cellular spermidine and is known to increase the content of S-adenosylmethionine decarboxylase protein, led to a 9-fold increase in the content of its mRNA, but treatment with methylglyoxal bis(guanylhydrazone) did not change the mRNA content.
D012437:Adenosylmethionine Decarboxylase / Q000235:genetics*; D000818:Animals; D002262:Carboxy-Lyases / Q000235:genetics*; D003513:Cycloheximide / Q000494:pharmacology; D000518:Eflornithine / Q000494:pharmacology*; D007527:Isoenzymes / Q000235:genetics; D007700:Kinetics; D008297:Male; D008970:Molecular Weight; D011061:Poly A / Q000235:genetics; D011467:Prostate / Q000187:drug effects / Q000201:enzymology*; D014176:Protein Biosynthesis / Q000187:drug effects*; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats
D007527:Isoenzymes; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D003513:Cycloheximide; D002262:Carboxy-Lyases; D012437:Adenosylmethionine Decarboxylase; D000518:Eflornithine