Autocrine growth function of human interleukin 1 molecules on ROHA-9, an EBV-transformed human B cell line.
In this study we report that the ROHA-9 cell line, an IL 1-secreting EBV-transformed human B cell line, exhibits an autocrine pathway of growth. In fact, ROHA-9 cells spontaneously secreted an autoregulatory growth factor that co-purified with the constitutively secreted IL 1-like molecules. Accordingly, monocyte-derived human IL 1, free of other known biological activities, also stimulated the growth of ROHA-9 cells in a dose-dependent way. Human recombinant interleukin 2, recombinant IFN-alpha or IFN-gamma and purified IFN-beta were ineffective when used at concentrations up to 1 X 10(3) U/ml. Furthermore, mouse recombinant IL 1, HPLC-purified multi-colony stimulating factor and partially purified preparations of BCGF were ineffective when assayed for growth-promoting activity on ROHA-9 cells. Moreover, a rabbit polyclonal antibody and a mouse monoclonal antibody to human IL 1 molecules blocked the growth of ROHA-9 cells induced by the autologous growth factor and by human IL 1. Lastly, purified human IL 1 increased the clonal efficiency of ROHA-9 cells seeded at a low cell concentration, allowing the isolation of the ROHA-9MC3 subclone, which showed similar growth response specificity and was particularly sensitive to the mitogenic activity of human IL 1.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002472:Cell Transformation, Viral; D005260:Female; D006133:Growth Substances / Q000276:immunology / Q000378:metabolism*; D004854:Herpesvirus 4, Human; D006801:Humans; D007372:Interferons / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology*; D008222:Lymphokines / Q000494:pharmacology; D051379:Mice; D008809:Mice, Inbred C3H
D000911:Antibodies, Monoclonal; D006133:Growth Substances; D007375:Interleukin-1; D008222:Lymphokines; D007372:Interferons