Inhibition of murine L cell interferon action by heparin.
Heparin added together with murine L cell interferon inhibited the development of antiviral activity in mouse L cells. When added after interferon treatment, heparin had no effect on antiviral activity. There was also no inhibition of interferon action in L cells treated with heparin before addition of interferon. On the other hand, heparin did not inhibit antiviral activities of human interferon alpha and beta. Since murine L cell interferon, but not human interferon alpha and beta, binds to a heparin affinity column and can be eluted with a solution of high salt, it is presumed that murine L cell interferon and heparin must interact with each other. The apparent interaction of heparin with murine L cell interferon was prevented by protamine, a drug that neutralizes heparin. Dextran sulfate inhibited murine L cell interferon action, but dextran and chondroitin sulfate A did not. These results suggests that heparin inhibited murine L cell interferon action by the binding via sulfate groups on its molecules. Heparin also inhibited antiviral activity of murine L cell interferon in mice infected with herpes simplex virus (+GC Miyama strain).
D000818:Animals; D002809:Chondroitin Sulfates / Q000494:pharmacology; D002846:Chromatography, Affinity; D016264:Dextran Sulfate; D003911:Dextrans / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology*; D006494:Heparin Antagonists / Q000494:pharmacology; D006561:Herpes Simplex / Q000188:drug therapy; D006801:Humans; D007370:Interferon Type I / Q000037:antagonists & inhibitors* / Q000627:therapeutic use; D007739:L Cells / Q000187:drug effects; D051379:Mice; D008809:Mice, Inbred C3H; D011479:Protamines / Q000494:pharmacology; D011971:Receptors, Immunologic / Q000187:drug effects; D017471:Receptors, Interferon; D014721:Vesicular stomatitis Indiana virus; D014779:Virus Replication / Q000187:drug effects
D003911:Dextrans; D006494:Heparin Antagonists; D007370:Interferon Type I; D011479:Protamines; D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D006493:Heparin; D002809:Chondroitin Sulfates; D016264:Dextran Sulfate