Typing of cytopathic and noncytopathic bovine viral diarrhea virus reference and Canadian field strains using a neutralizing monoclonal antibody.
Cytopathic and noncytopathic reference strains as well as Canadian field isolates of bovine viral diarrhea virus were analyzed by neutralization and immunofluorescence tests using a bovine viral diarrhea virus-specific neutralizing monoclonal antibody. Results on reference strains indicated three major antigenic groups: I) NADL-like, II) New York 1-like and III) Oregon C24V-like. Field isolates could be segregated into groups I and II and none could be typed into the group III. It appears that most bovine viral diarrhea virus strains share a common antigen which carries a major neutralization epitope. These characteristics would make this monoclonal antibody a useful reagent for taxonomic and epizootiological studies.
D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis*; D002417:Cattle; D003588:Cytopathogenic Effect, Viral; D001908:Diarrhea Viruses, Bovine Viral / Q000145:classification* / Q000276:immunology; D000939:Epitopes / Q000032:analysis; D005455:Fluorescent Antibody Technique; D009500:Neutralization Tests; D010576:Pestivirus / Q000145:classification*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes