A monoclonal antibody reacting with human basophils.
Bsp-1 is an IgM murine monoclonal antibody raised against the human erythroblastic leukemia cell line (HEL) that reacts with basophils but not neutrophils or eosinophils. Western blotting techniques showed that Bsp-1 reacts with a 45-kilodalton surface antigen on HEL cells. The distribution of Bsp-1 antigen on leukemic cells is confined to a basophilic leukemia cell line, KU812, chronic myeloid leukemia with basophilia, and some cases of acute undifferentiated leukemia. Bsp-1 might therefore be a useful reagent for the study of basophil function and differentiation.
D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface / Q000032:analysis*; D001491:Basophils / Q000166:cytology / Q000276:immunology* / Q000502:physiology; D002454:Cell Differentiation; D002460:Cell Line; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006651:Histocytochemistry; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology; D007951:Leukemia, Myeloid / Q000473:pathology; D010641:Phenotype
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000939:Epitopes; D003101:Collodion