Irreversible in vivo and in vitro binding of thiabendazole to tissue protein of pregnant mice.
In vivo experiments showed that [14C]thiabendazole is irreversibly bound to protein of the liver and embryo in pregnant mice, but not to DNA and RNA in these tissues. In experiments carried out in vitro, the binding of [14C]thiabendazole to microsomal protein of the liver was proportional to time and protein concentration, was dependent upon the presence of oxygen and NADPH and was inhibited by carbon monoxide or SKF-525A, indicating that it was mediated by the cytochrome P-450-dependent monooxygenase system. The binding capacity of microsomes in various tissues was examined, and that of the liver microsomes was found to be more pronounced than that of other tissues.
D000818:Animals; D004247:DNA / Q000378:metabolism; D005260:Female; D005333:Fetus / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000378:metabolism; D010634:Phenobarbital / Q000494:pharmacology; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D011335:Proadifen / Q000494:pharmacology; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D013827:Thiabendazole / Q000378:metabolism*
D011506:Proteins; D012313:RNA; D004247:DNA; D011335:Proadifen; D013827:Thiabendazole; D010634:Phenobarbital