Antibodies of predetermined specificity to apolipoprotein C-II elicited with a synthetic peptide. Characterization and use for immunoassays.
Antibodies of predetermined specificity raised against a synthetic peptide corresponding to the C-terminal region of apolipoprotein C-II (Apo C-II) 63-79 were shown to be specific for the apolipoprotein by Western blot. The recognition by these antibodies of Apo C-II containing lipoprotein particles (both isolated and in plasma) was studied in a fluid-phase radioimmunoassay and the affinity constant for plasma was determined. The role of lipids in the expression of epitopes was studied by comparing the antigenicity of intact and delipidated Apo C-II containing fractions. The antibodies proved to be as suitable as conventional anti-protein antibodies in an immunoenzymometric assay and, moreover, were able to develop 'rockets' in an electroimmunoassay.
D000906:Antibodies / Q000302:isolation & purification*; D000915:Antibody Affinity; D000918:Antibody Specificity; D053304:Apolipoprotein C-II; D001056:Apolipoproteins C / Q000097:blood / Q000276:immunology*; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods; D007124:Immunoenzyme Techniques; D008075:Lipoproteins, HDL / Q000502:physiology; D008079:Lipoproteins, VLDL / Q000502:physiology; D010446:Peptide Fragments / Q000276:immunology*
D000906:Antibodies; D053304:Apolipoprotein C-II; D001056:Apolipoproteins C; D000939:Epitopes; D008075:Lipoproteins, HDL; D008079:Lipoproteins, VLDL; D010446:Peptide Fragments