Crosslinking of ribosomal protein S18 to 16 S RNA in E.coli ribosomal 30 S subunits by the use of a reversible crosslinking agent: trans-diamminedichloroplatinum(II).
We have previously developed [(1987) Biochemistry 26, 5200-5208] the use of trans-diamminedichloroplatinum(II) to induce reversible RNA-protein crosslinks in the ribosomal 30 S subunit. Protein S18 and, to a lesser extent, proteins S13/S14, S11, S4 and S3 could be crosslinked to the 16 S rRNA. The aim of the present work was to identify the crosslinking sites of protein S18. Three sites could be detected: a major one located in region 825-858, and two others located in regions 434-500 and 233-297. This result is discussed in the light of current knowledge of the topographical localization of S18 in the 30 S subunit and of its relation with function.
D001483:Base Sequence; D001665:Binding Sites; D002352:Carrier Proteins / Q000032:analysis; D002945:Cisplatin; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000032:analysis*; D009841:Oligonucleotides / Q000032:analysis; D012329:RNA, Bacterial / Q000032:analysis; D012269:Ribosomal Proteins / Q000032:analysis
D002352:Carrier Proteins; D003432:Cross-Linking Reagents; D009841:Oligonucleotides; D012329:RNA, Bacterial; D012269:Ribosomal Proteins; C023509:ribosomal protein S16; C041184:ribosomal protein S18; C040469:ribosomal protein S30; C034697:transplatin; D002945:Cisplatin