[Isolation and regeneration of the protoplasts of the streptomycete producers of actinomycins C and X].
Protoplasts of S. michiganensis, S. chrysomallus and Streptomyces sp. 26-115, organisms producing actinomycins C and X form in hypertonic salt solution under the action of 3-4,5 mg/ml of lysozyme on the mycelium suspension. For protoplasting, the streptomycetes were grown on the soybean medium in the presence of 0.2-0.8 per cent of glycine. The mycelium of the streptomycete exponential growth phase was more favourable for protoplast formation. Protoplast regeneration was studied on the medium described by Okanishi et al. The quantitative composition of this medium was not optimal for regeneration of protoplasts of the above streptomycetes. The level of their regeneration depended to various extents on concentration of phosphate, magnesium and calcium ions and sucrose in the regeneration medium.
D003470:Culture Media; D003609:Dactinomycin / Q000031:analogs & derivatives* / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D011523:Protoplasts / Q000187:drug effects / Q000502:physiology*; D013302:Streptomyces / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*
D003470:Culture Media; C030013:cactinomycin; D003609:Dactinomycin; C041783:actinomycin X
Poluchenie i regeneratsiia protoplastov streptomitsetov--produtsentov aktinomitsinov C i X.