Development of a defined medium and two-step culturing method for improved exotoxin A yields from Pseudomonas aeruginosa.
A two-step method is described for the production of exotoxin A by Pseudomonas aeruginosa in which a defined growth medium is modified for the toxin production phase. As a result, specific exotoxin A yields comparable to those obtained with complex media were achieved. In the development of this two-step process, several divalent metallic cations (Ca2+, Cu2+, and Mn2+), in addition to iron, were found to inhibit the yield of exotoxin A while Ca2+ and glycerol were found to increase yields. Northern blot analysis of total RNA isolates suggests that these effects on exotoxin A yields are based on events at the transcription level.
D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D002118:Calcium / Q000494:pharmacology; D002413:Cations, Divalent / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology; D003470:Culture Media; D005098:Exotoxins / Q000096:biosynthesis* / Q000235:genetics; D005798:Genes, Bacterial; D005990:Glycerol / Q000378:metabolism; D007118:Immunoassay; D007501:Iron / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D012329:RNA, Bacterial / Q000032:analysis; D014158:Transcription, Genetic / Q000187:drug effects; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D002118:Calcium; D002413:Cations, Divalent; D003300:Copper; D003470:Culture Media; D005098:Exotoxins; D005990:Glycerol; D007501:Iron; D008345:Manganese; D012329:RNA, Bacterial; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A