Substance P modulates the release of locally synthesized nerve growth factor from rat saphenous nerve neuroma.
In saphenous nerve neuromata of adult rats a long-term increase of immunoreactive nerve growth factor (irNGF) was detected after nerve transection. The occurrence of messenger RNA encoding NGF (NGF mRNA) in these proximal nerve stumps indicates local biosynthesis of NGF. In situ superfusion of neuromata revealed a constant release of irNGF which was significantly reduced by substance P (SP) but not affected by calcitonin gene related peptide (CGRP). We therefore suggest that SP may modulate the availability of NGF in the microenvironment of the regenerating nerve fiber endings.
D000818:Animals; D015740:Calcitonin Gene-Related Peptide; D006614:Hindlimb / Q000294:innervation; D008297:Male; D009414:Nerve Growth Factors / Q000235:genetics / Q000378:metabolism*; D009463:Neuroma / Q000378:metabolism*; D009479:Neuropeptides / Q000494:pharmacology; D010525:Peripheral Nerves / Q000032:analysis / Q000601:surgery; D010524:Peripheral Nervous System Neoplasms / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013373:Substance P / Q000494:pharmacology*
D009414:Nerve Growth Factors; D009479:Neuropeptides; D012333:RNA, Messenger; D013373:Substance P; D015740:Calcitonin Gene-Related Peptide