[Arg3]substance P and neurokinin A from chicken small intestine.
Using antisera specific for NH2-terminal and COOH-terminal regions of substance P and for the COOH-terminal region of neurokinin A, peptides with tachykinin-like immunoreactivity were isolated from extracts of chicken small intestine. The peptide Arg-Pro-Arg-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 differs from human substance P by substitution of the lysyl residue by an arginyl residue at position 3. Synthetic [Arg3]substance P showed identical chromatographic and immunochemical properties to chicken substance P and was equipotent with substance P in contracting the guinea pig ileum. A second peptide His-Lys-Thr-Asp-Ser-Phe-Val-Gly-Leu-Met-NH2 isolated from the extracts is identical to human neurokinin A. A third peptide was immunoreactive towards the COOH-terminally directed anti-serum to substance P only but was not characterized structurally in this study.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002645:Chickens; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D007120:Immunochemistry; D007421:Intestine, Small / Q000032:analysis*; D015288:Neurokinin A; D009479:Neuropeptides / Q000276:immunology / Q000302:isolation & purification*; D013373:Substance P / Q000031:analogs & derivatives* / Q000276:immunology / Q000302:isolation & purification
D000596:Amino Acids; D009479:Neuropeptides; C055563:substance P, Arg(3)-; D013373:Substance P; D015288:Neurokinin A