Resolution and identification of O-phosphoserine, O-phosphothreonine, O-phosphotyrosine, and gamma-carboxyglutamic acid as their fluorescent o-phthalaldehyde derivatives by high performance liquid chromatography.
High performance liquid chromatography was used to resolve O-phosphoserine, O-phosphothreonine, and O-phosphotyrosine as their fluorescent o-phthalaldehyde derivatives. By adjusting the buffer system, very small amounts of O-phosphothreonine could be detected and quantitated in the presence of very large amounts of O-phosphoserine. In addition, gamma-carboxyglutamic acid and glutamic acid were also separated and quantitated. Depending on the buffer used, various combinations of these amino acids could be resolved in a single run.
D015055:1-Carboxyglutamic Acid / Q000302:isolation & purification*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005456:Fluorescent Dyes; D010768:Phosphoserine / Q000302:isolation & purification*; D010769:Phosphothreonine / Q000302:isolation & purification*; D019000:Phosphotyrosine; D012694:Serine / Q000031:analogs & derivatives*; D013912:Threonine / Q000031:analogs & derivatives*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000302:isolation & purification; D009764:o-Phthalaldehyde
D005456:Fluorescent Dyes; D010769:Phosphothreonine; D010768:Phosphoserine; D019000:Phosphotyrosine; D013912:Threonine; D014443:Tyrosine; D012694:Serine; D015055:1-Carboxyglutamic Acid; D009764:o-Phthalaldehyde