Use of an enzyme linked immunosorbent assay (ELISA) for quantification of proteins on the surface of materials.
This study demonstrates the usefulness of an enzyme linked immunosorbent assay (ELISA) for detection and quantification of protein on the surface of materials. Bovine serum albumin (BSA) and bovine gamma globulin (BGG) were the proteins used. Titanium and stainless steel were the materials tested. The proteins were detected with the use of rabbit antiserum specific for BSA and for BGG. This reaction was quantitated by the use of horseradish peroxidase conjugated goat anti-rabbit gamma globulin. The technique is described in detail. The technique was demonstrated to be suitable for quantitation of protein from 0.01 mg/mL to 0.1 mg/mL on the surface of 3 mm X 10 mm materials. The technique was also demonstrated to be suitable for determining the surface area of solid materials. It is a simple technique and suitable for most biomaterials laboratories.
D000327:Adsorption; D000818:Animals; D001667:Binding, Competitive; D001672:Biocompatible Materials; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D011485:Protein Binding; D012044:Regression Analysis; D012710:Serum Albumin, Bovine / Q000032:analysis*; D013193:Stainless Steel; D014025:Titanium; D005719:gamma-Globulins / Q000032:analysis*
D001672:Biocompatible Materials; D005719:gamma-Globulins; D013193:Stainless Steel; D012710:Serum Albumin, Bovine; D014025:Titanium