Specific activity staining for prostaglandin metabolizing enzymes on polyacrylamide gel.
A simple activity staining technique on polyacrylamide gel for 15-hydroxyprostaglandin metabolizing enzymes was developed in which production or digestion of 15-keto-PGE was stained. The rapidity and specificity of this staining was shown with a preparation of NAD-linked 15-hydroxyprostaglandin dehydrogenase isolated from human term placenta.
D004796:Clinical Enzyme Tests / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005260:Female; D006801:Humans; D006911:Hydroxyprostaglandin Dehydrogenases / Q000302:isolation & purification / Q000378:metabolism; D010920:Placenta / Q000201:enzymology; D011453:Prostaglandins / Q000378:metabolism*; D013194:Staining and Labeling / Q000379:methods
D011453:Prostaglandins; D006911:Hydroxyprostaglandin Dehydrogenases; C035859:15-hydroxyprostaglandin dehydrogenase