Macromolecular prodrugs. V. Simultaneous determination of high-molecular-weight dextran metronidazole monosuccinate ester prodrugs and the hydrolysis products metronidazole and the corresponding monosuccinate ester on nucleosil diol.
A high-performance size-exclusion chromatography procedure using Nucleosil Diol has been developed which provides the simultaneous determination of macromolecular dextran metronidazole monosuccinate ester prodrugs and the hydrolysis products metronidazole and metronidazole monosuccinate. Various factors influencing the chromatographic behaviour of the compounds are discussed. Baseline separation of the three substances was achieved within 8 min by using a 0.05 M phosphate buffer pH 7.5 eluent at a flow-rate of 2 ml min-1. The detection limit at 320 nm for a conjugate with a degree of substitution of 4.61 was found to be 3.5 micrograms ml-1.
D002850:Chromatography, Gel; D003911:Dextrans / Q000032:analysis*; D006868:Hydrolysis; D007700:Kinetics; D008795:Metronidazole / Q000031:analogs & derivatives* / Q000032:analysis*; D012822:Silicon Dioxide / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D003911:Dextrans; C051965:metronidazole monosuccinate-dextran conjugate; D008795:Metronidazole; D012822:Silicon Dioxide