[Detection and purification of a liver microsomal enzyme inducing the release of a glycopeptide inhibitor of hepatocyte proliferation from human alpha 2 macroglobulin].
The interaction rat liver microsomes/human alpha 2 macroglobulin releases in vivo an inhibitory peptide of the hepatocyte proliferation. Treatment of Triton X 100 on adult rat liver microsomes enables the solubilisation of a proteolytic enzyme. Its partial purification was obtained by Ultrogel AcA 44 filtration followed by a DEAE Sephacel chromatography. This enzyme shows a proteolytic activity in presence of calcium on synthetic substrates including Phe, Tyr and Trp. A whole enzyme inhibition is got after treatment by DFP or benzamidine. In presence of highly purified human alpha 2 macroglobulin this enzyme releases a glycopeptide of low molecular weight, which inhibits the hepatocyte proliferation during the G1-S transition in baby rat.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003902:Detergents; D006020:Glycopeptides / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008099:Liver / Q000166:cytology* / Q000187:drug effects; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000302:isolation & purification* / Q000378:metabolism; D051381:Rats; D000511:alpha-Macroglobulins / Q000378:metabolism*
D003902:Detergents; D006020:Glycopeptides; D000511:alpha-Macroglobulins; D010447:Peptide Hydrolases
Détection et purification d'une enzyme microsomiale hépatique induisant la libération du glycopeptide inhibiteur de la prolifération des hépatocytes à partir de l'alpha 2 macroglobuline humaine.