Studies on the Vibrio cholerae mucinase complex. II. Specific neuraminidase activity measured histochemically in a goblet cell assay.
The activity of neuraminidase prepared from the mucinase complex of Vibrio cholerae was measured by a new, semi-quantitative goblet-cell assay. The counts of normal, alcianophilic, sialomucin-containing goblet cells (purple-stained) and neutral mucosubstance-containing goblet cells (magenta-stained) in serial sections of ileum were compared before and after neuraminidase treatment. The procedure provides a more natural assessment of the action of V. cholerae neuraminidase on the viscous intestinal mucus.
D000423:Alcian Blue; D000818:Animals; D006168:Guinea Pigs; D006651:Histocytochemistry; D007082:Ileum / Q000032:analysis; D009439:Neuraminidase / Q000032:analysis* / Q000494:pharmacology; D011133:Polysaccharide-Lyases / Q000032:analysis*; D011817:Rabbits; D051381:Rats; D013194:Staining and Labeling; D014734:Vibrio cholerae / Q000201:enzymology*
D009439:Neuraminidase; D011133:Polysaccharide-Lyases; C100002:hyaluronate lyase; D000423:Alcian Blue