c-myc oncogene expression inhibits the initiation of myogenic differentiation.
The role of c-myc oncogene expression in myogenic differentiation has been established by transfecting rat myoblasts of the L6 cell line with plasmid pMT-myc, in which the c-myc coding sequences were under the control of the metallothionein I promoter. We observed that the constitutive expression of the exogenous c-myc gene inhibits muscular differentiation. A diminution of the endogenous c-myc gene expression occurs within the first 24 h after the transfer of the cells to a differentiating medium. This early decrease of c-myc expression is required for cell differentiation to occur. We have also observed that exogenous myc gene expression has no effect on endogenous myc expression.
D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D004274:DNA, Recombinant; D009132:Muscles / Q000166:cytology*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011519:Proto-Oncogenes; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D051381:Rats; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D012313:RNA