A nuclear factor which interacts with an AT-cluster in the first intron of rat alpha 2-macroglobulin gene.
We have identified a nuclear protein which binds specifically to the first intron of rat alpha 2-macroglobulin gene. The protein became insoluble at low salt concentration retaining the binding specificity. Its molecular weight was estimated to be 22 kilodalton by a protein blotting procedure. The binding site of the protein determined by DNase I footprinting was an AT-strech which shared 80% homology with the cleavage consensus of Drosophila topoisomerase II.
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002467:Cell Nucleus / Q000032:analysis*; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes; D004268:DNA-Binding Proteins / Q000378:metabolism*; D003850:Deoxyribonuclease I; D007438:Introns; D008099:Liver / Q000648:ultrastructure*; D008970:Molecular Weight; D010957:Plasmids; D011401:Promoter Regions, Genetic; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D012995:Solubility; D000511:alpha-Macroglobulins / Q000235:genetics*
D004268:DNA-Binding Proteins; D000511:alpha-Macroglobulins; D004247:DNA; D004262:DNA Restriction Enzymes; D003850:Deoxyribonuclease I