title: Sodium channels in cardiac Purkinje cells.
abstract: Sodium (Na+) currents are responsible for excitation and conduction in most cardiac cells, but their study has been hampered by the lack of a satisfactory method for voltage clamp. We report a new method for low resistance access to single freshly isolated canine cardiac Purkinje cells that permits good control of voltage and intracellular ionic solutions. The series resistance was usually less than 3 omega cm2, similar to that of the squid giant axon. Cardiac Na+ currents resemble those of nerve. However, Na+ current decay is multiexponential. The basis for this was further studied with cell-attached patch clamp recording of single Na+ channel properties. A prominent characteristic of the single channels was their ability to reopen after closure. There was also a long opening state that may be the basis for a small very slowly decaying Na+ current. This rare long opening state may contribute to the Na+ current during the action potential plateau.
mesh_terms: D000200:Action Potentials; D000818:Animals; D004285:Dogs; D004553:Electric Conductivity; D004594:Electrophysiology; D006329:Heart Conduction System / Q000502:physiology*; D007473:Ion Channels / Q000502:physiology*; D010477:Perfusion; D011690:Purkinje Fibers / Q000502:physiology*; D012964:Sodium / Q000378:metabolism*
chemical_list: D007473:Ion Channels; D012964:Sodium