Detection of glycocalyx of the pancreatic acinar cells in rats by intraductal injections of different markers into the pancreatic duct system.
The glycocalyx of the pancreatic acinar cells might play a role in the pathogenesis of ductally induced pancreatitis. The detection of luminar glycocalyx of pancreatic acinar and ductular cells succeeded by injections of different markers, such as ruthenium red, lanthanum hydroxide, tannic acid, colloidal iron or ferritin in the pancreatic duct system. The markers enter also the interstitium by local ruptures in the junctional complexes and cause a contrastation of basolateral glycocalyx as well as the basal lamina and of collagen fibres. There were differences between the apical and basolateral glycocalyx that are discussed with supposed differences of physicochemical properties and of different composition of carbohydrates as well as of specialized functions of membrane compartments.
D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure*; D006023:Glycoproteins / Q000032:analysis*; D008297:Male; D008854:Microscopy, Electron; D010179:Pancreas / Q000648:ultrastructure*; D011134:Polysaccharides / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling
D006023:Glycoproteins; D011134:Polysaccharides