title: Recovery of protein from tear fluid stored in cellulose sponges.
abstract: Human tear fluid collected in glass capillaries was absorbed in cellulose sponges. After various time intervals the recovery was measured for total protein, amylase (AMY), lysozyme (LZM), immunoglobulin A (IgA) and lactoferrin (LF). After one day storage at 4 degrees C of moistened sponges of at least 92% recovery was observed. Storage for longer periods at 4 degrees C up to seven days showed a decreasing recovery for total protein, AMY and IgA. Tear collection with sponges and storage of wet sponges for maximally one day at 4 degrees C followed by centrifugation or storage of the fluid at -20 degrees C are reliable ways of sample treatment.
mesh_terms: D000042:Absorption; D000681:Amylases / Q000378:metabolism; D002482:Cellulose; D005136:Eye Proteins / Q000378:metabolism*; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism; D007781:Lactoferrin / Q000378:metabolism; D009113:Muramidase / Q000378:metabolism; D011309:Preservation, Biological; D013666:Tears / Q000201:enzymology / Q000378:metabolism*
chemical_list: D005136:Eye Proteins; D007070:Immunoglobulin A; D002482:Cellulose; D000681:Amylases; D009113:Muramidase; D007781:Lactoferrin