The effects of the main body cations on the peptidase(s) activity against pyroGlu6[125I-Tyr8]SP6-11 in the nuclear and synaptosomal fraction of the cortex and hippocampus of rat brain.
1. Peptidase(s) activity of nuclear and synaptosomal fraction from cortex and hippocampus of rat brain against pyroGlu6[125I-Tyr8]SP6-11 was evaluated in different concentration of Ca2+, Mg2+, K+ and Na+ in about "isotonic" conditions. 2. The effects of studied ions on the peptidase activities forming N-terminal and C-terminal fragments are different especially in synaptosomes of both areas. 3. The differences of ionic requirements for N- and C-forming activities are particularly relevant for Ca2+ at the cortex and K+ at the hippocampus. 4. Ca2+ activate forming of N-terminal fragments in the nuclear fraction whereas inhibit it in synaptosomes from both areas. 5. The ionic requirements for C-terminal fragments' formation in synaptosomes of both areas are contradictory.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002412:Cations / Q000494:pharmacology*; D002467:Cell Nucleus / Q000201:enzymology; D002540:Cerebral Cortex / Q000201:enzymology*; D005260:Female; D006624:Hippocampus / Q000201:enzymology*; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D009994:Osmolar Concentration; D010446:Peptide Fragments / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000494:pharmacology; D013373:Substance P / Q000031:analogs & derivatives* / Q000378:metabolism; D013574:Synaptosomes / Q000201:enzymology
D002412:Cations; D007457:Iodine Radioisotopes; D010446:Peptide Fragments; D013373:Substance P; C026238:substance P (6-11), pGlu(6)-; D012964:Sodium; D010447:Peptide Hydrolases; D008274:Magnesium; D011188:Potassium; D002118:Calcium; D011761:Pyrrolidonecarboxylic Acid