title: Use of an Epstein-Barr virus episomal replicon for anti-sense RNA-mediated gene inhibition in a human cytotoxic T-cell clone.
abstract: A methodology was developed for stable gene transfer into cloned nontransformed human T lymphocytes. Stable high-level gene expression was achieved in cloned human T cells by using a self-replicating Epstein-Barr virus (EBV) episomal replicon. A comparison of five eukaryotic promoters established that the Rous sarcoma virus 3' long terminal repeat (RSV 3' LTR) and the lymphopapilloma virus (LPV) 5' LTR are optimal for episome-based expression in T cells. Effective (greater than 95%), selective, and reversible anti-sense RNA-mediated gene inhibition of a model T-cell-associated molecule (CD8) was achieved in a cytotoxic human T-cell clone by using an EBV episome-based, RSV 3' LTR-driven expression system. The linking of anti-sense RNA mutagenesis and T-cell cloning technologies should contribute significantly to studies of human T-cell function.
mesh_terms: D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics*; D016827:CD8 Antigens; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D004854:Herpesvirus 4, Human / Q000235:genetics*; D010957:Plasmids; D012313:RNA / Q000235:genetics; D018075:RNA, Complementary; D012093:Replicon; D013602:T-Lymphocytes, Cytotoxic / Q000502:physiology*; D014162:Transfection
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens; D018075:RNA, Complementary; D012313:RNA