Structure of the antigenic O-polysaccharide of the lipopolysaccharide produced by Salmonella eimsbuttel.
The smooth lipopolysaccharide produced by Salmonella eimsbuttel, which had the O:6, O:7, and O:14 antigenic factors defined in the Kauffmann-White classification, was shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis, composition analysis, methylation, periodate oxidation, deamination, and 1H and 13C nuclear magnetic resonance studies to contain a high molecular weight O-chain polysaccharide composed of D-mannose (four parts), D-glucose (one part), and 2-acetamido-2-deoxy-D-glucose (one part). It was a branched polymer of a repeating hexasaccharide unit having the structure (formula; see text).
D000942:Antigens, Bacterial / Q000032:analysis*; D002240:Carbohydrate Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D008070:Lipopolysaccharides / Q000032:analysis / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D019081:O Antigens; D012475:Salmonella / Q000276:immunology*
D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D019081:O Antigens