Molecular regulation of ethanol-inducible cytochrome P450-IIEI in hamsters.
Liver polysomal poly(A)+ RNA, isolated from hamsters treated with ethanol or pyrazole, was translated in vitro to determine the effect of these compounds on specific mRNA encoding P450-IIEI, an ethanol-inducible P450 isozyme. As assessed by immunoprecipitation of translation products, ethanol and pyrazole increased hepatic P450-IIEI mRNA levels by 160% and 45%, respectively, when compared to controls. In liver microsomes from the same animals, ethanol and pyrazole caused a two-fold increase in microsomal P450-IIEI protein and a two- to three-fold enhancement of microsomal ethanol oxidation and p-nitrophenol hydroxylation. Our results show that the induction of P450-IIEI protein in hamsters by ethanol and pyrazole, an "ethanol-like" inducer, is accompanied by an increase in translatable P450-IIEI mRNA.
D000818:Animals; D006224:Cricetinae; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis* / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D000431:Ethanol / Q000494:pharmacology*; D007163:Immunosorbent Techniques; D007527:Isoenzymes / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D008647:Mesocricetus; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D011061:Poly A / Q000378:metabolism; D014176:Protein Biosynthesis; D011720:Pyrazoles / Q000494:pharmacology; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*
D007527:Isoenzymes; D011720:Pyrazoles; D012333:RNA, Messenger; D011061:Poly A; D000431:Ethanol; C031280:pyrazole; D012313:RNA; D003577:Cytochrome P-450 Enzyme System