Purification and characterization of human skin mast cells. Evidence for human mast cell heterogeneity.
Our previous studies of human lung and intestinal mast cells failed to show the heterogeneity found among mast cells in murine species. Recently, we and others have developed techniques for the enzymatic dispersion of human neonatal skin mast cells. In addition, we are now able to make single cell suspensions of mast cells from adult skin and to purify these cells to near homogeneity. Comparative studies of mast cells from these several sources have uncovered several major differences among them. Adult and neonatal skin mast cells themselves differ in that the former are 10-fold less sensitive to goat anti-human IgE, with maximal release occurring at 3.0 and 0.3 microgram/ml, respectively. Skin mast cells also differ in optimal temperature for release: adult mast cells respond maximally at 23 to 30 degrees C and neonatal cells at 37 degrees C. Skin mast cells from both sources are dramatically different from lung and intestinal mast cells in two aspects. First, skin mast cells are quite responsive to several stimuli--morphine sulfate (10(-4) to 10(-6) M), substance P (10(-5) to 10(-7) M), compound 48/80 (10 to 0.1 microgram/ml), f-Met peptide (10(-6) M), and C5a (10(-8) M)--to which the other mast cells fail to respond. Second, although stimulated skin mast cells produce prostaglandin D2, little leikotriene C4, if any, is generated, unlike lung or intestinal mast cells. These differences in inflammatory potential among human mast cells from various sites have important implications for the management of allergic and inflammatory responses.
D002118:Calcium / Q000502:physiology; D002469:Cell Separation; D003182:Complement C5 / Q000494:pharmacology; D015936:Complement C5a; D006636:Histamine Release; D006801:Humans; D007073:Immunoglobulin E / Q000502:physiology; D007231:Infant, Newborn; D008168:Lung / Q000166:cytology; D008407:Mast Cells / Q000166:cytology* / Q000502:physiology; D009239:N-Formylmethionine / Q000494:pharmacology; D015230:Prostaglandin D2; D011457:Prostaglandins D / Q000096:biosynthesis; D013189:SRS-A / Q000096:biosynthesis; D012867:Skin / Q000166:cytology*; D013373:Substance P / Q000502:physiology
D003182:Complement C5; D011457:Prostaglandins D; D013189:SRS-A; D013373:Substance P; D007073:Immunoglobulin E; D009239:N-Formylmethionine; D015936:Complement C5a; D015230:Prostaglandin D2; D002118:Calcium