Liquid competition radioimmunoassay for the detection and quantitation of the HIV p24.
Productive infection of permissive cell cultures by HIV has been detected by different assays of which the measurement of reverse transcriptase (RT) activity has been considered highly specific and sensitive. Here we describe the production and characterization of a mouse hybridoma cell line, MB12, secreting monoclonal antibodies to HIV p24, the major core protein, and the use of this monoclonal antibody to develop a type specific indirect liquid competitive radioimmunoassay (RIA) capable of providing earlier detection of the replicating virus than the RT assay. This assay also provides a quantitative analysis of HIV p24, which can be used to study the viral replication in permissive cell cultures. The ease of methodology and the adaptability of the competitive RIA to various assay conditions make this immunoassay suitable for the study of HIV expression in infected cell cultures.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D006678:HIV / Q000032:analysis* / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D012194:RNA-Directed DNA Polymerase / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods*; D014758:Viral Core Proteins / Q000032:analysis*
D000911:Antibodies, Monoclonal; D014758:Viral Core Proteins; D011092:Polyethylene Glycols; D017830:Octoxynol; D012194:RNA-Directed DNA Polymerase