[Template-independent incorporation of nucleotides into low-molecular weight RNA in a cell-free system].
Small RNAs were studied in the cell-free system containing cytosol and nucleoside triphosphates. The specific incorporation of NMPs into small RNAs was shown. While AMP and CMP were incorporated predominantly into tRNA, UMP was incorporated into 4.5S, 5S, and U1 RNAs. GMP was not incorporated into small RNAs in this system. The data suggest that maturation of 4.5S, 5S, and U1 RNAs involves post-transcriptional addition of uridylate residues at their 3'-ends.
D000818:Animals; D002474:Cell-Free System; D008970:Molecular Weight; D009711:Nucleotides / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis*; D013698:Templates, Genetic; D014158:Transcription, Genetic
D009711:Nucleotides; D012313:RNA
Nezavisimoe ot matritsy vkliuchenie nukleotidov v nizkomolekuliarnye RNK v beskletochnoĭ sisteme.