[Cytophotometric analysis of the protein and nucleic acid content of the nucleus and cytoplasm of resting and proliferation-activated hepatocytes in the mouse].
Mouse hepatocytes, quiescent and activated to proliferation after hepatectomy, were investigated using cytophotometry in the ultraviolet region of selective absorbtion of nucleic acids, and then were examined in the visible region of spectrum after cytochemical reaction for total protein. Some features of RNA and protein accumulation were revealed in the nucleus and cytoplasm of activated cells of different ploidy. The activation resulted in the reduction of histone content and in the increase in the nucleus: cytoplasm ratio in cells of all the ploidy classes.
D000818:Animals; D002455:Cell Division; D002467:Cell Nucleus / Q000032:analysis / Q000378:metabolism; D003592:Cytophotometry; D003593:Cytoplasm / Q000032:analysis / Q000378:metabolism; D004247:DNA / Q000032:analysis* / Q000378:metabolism; D006498:Hepatectomy; D006651:Histocytochemistry; D006657:Histones / Q000032:analysis / Q000378:metabolism; D008099:Liver / Q000378:metabolism / Q000648:ultrastructure*; D051379:Mice; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D012313:RNA / Q000032:analysis* / Q000378:metabolism; D051381:Rats; D013997:Time Factors
D006657:Histones; D011506:Proteins; D012313:RNA; D004247:DNA
Tsitofotometricheskiĭ analiz soderzhaniia belkov i nukleinovykh kislot v iadre i tsitoplazme pokoiashchikhsia i aktivirovannykh k proliferatsii gepatotsitov myshi.