[Threonyl-tRNA synthase from rabbit reticulocytes: a reversible transition from the RNA-non-binding to the RNA-binding form].
A simple three-step procedure was used to isolate threonyl-tRNA synthetase of rabbit reticulocytes which is in a ribosome-free extract in the RNA-non-binding form. According to SDS electrophoresis, the enzyme has a molecular weight of 86 000 Da and is heterogeneous by isoelectric point; pI of the major component is near 6.2. Threonyl-tRNA synthetase is capable of interacting with a high molecular weight RNA (E. coli rRNA). Thus, in the course of purification threonyl-tRNA synthetase passes from the RNA-non-binding to the RNA-binding form. This transition was shown to be reversible.
D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D012329:RNA, Bacterial / Q000378:metabolism; D012335:RNA, Ribosomal / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes / Q000201:enzymology*; D013914:Threonine-tRNA Ligase / Q000302:isolation & purification / Q000378:metabolism*
D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D000604:Amino Acyl-tRNA Synthetases; D013914:Threonine-tRNA Ligase
Treonil-tRNK-sintetaza retikulotsitov krolika: obratimyĭ perekhod iz RNK-nesviazyvaiushcheĭ v RNK-sviazyvaiushchuiu formu.