[RNA analysis of the measles virus in a human cell culture of the chronic infection].
Synthesis of virus-specific RNAs in human HEP-2 and L-41 cells chronically infected with measles virus was studied in comparison with synthesis of viral RNA in acutely infected L-41 cells. The RNA, a component of RNP isolated from chronically infected cells, was shown to be represented mainly by "minus" chains and to contain 23-25% "plus"-RNA. It was demonstrated by blotting hybridization that 1 species of genomic RNA with a molecular weight of 5 megadaltons was synthesized in acute infection whereas in chronically infected cells a small amount of subgenomic RNAs was additionally detected in RNP. The level of virus genome transcription in chronically infected cells was 7-8 fold lower than that in acute infection. The RNA-transcriptase activity of RNP isolated from chronically infected HEP-2 and L-41 cells was also lower than RNP activity from acutely infected L-41 cells. The observed features of virus-specific RNA synthesis in chronically infected cells seem to be likely to play a role in the maintenance of virus persistence.
D002460:Cell Line; D002908:Chronic Disease; D004247:DNA / Q000032:analysis / Q000302:isolation & purification; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D005814:Genes, Viral; D006801:Humans; D008457:Measles / Q000382:microbiology*; D008459:Measles virus / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D008722:Methods; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis* / Q000096:biosynthesis / Q000302:isolation & purification; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012261:Ribonucleoproteins / Q000032:analysis / Q000302:isolation & purification; D014776:Virus Cultivation
D004279:DNA, Viral; D012367:RNA, Viral; D012261:Ribonucleoproteins; D004247:DNA; D012194:RNA-Directed DNA Polymerase
Analiz RNK virusa kori v kul'ture kletok cheloveka pri khronicheskoĭ infektsii.