Inhibition of the erythrocyte Na+, K+-pump by mammalian lignans.
Several mammalian lignans, particularly enterolactone, 3-oxy-methyl enterolactone and prestegane B are able to inhibit Na+, K+-pump activity in human red cells with IC50 of about 1 mM. The inhibition of Na+, K+-pump activity by mammalian lignans have the following properties: the IC50 for ouabain remains unchanged suggesting a noncompetitive inhibition. The apparent affinity for internal Na+ and maximal rate of cation translocation are both diminished. The above inhibition of the Na+, K+-pump was obtained at doses 2-3 orders of magnitude higher than those required for ouabain. However we cannot exclude that a glycosyl- (and/or butenolide)-derivative of enterolactone could be one endogenous ouabain-like factor.
D003429:Cross Reactions; D004077:Digoxin / Q000032:analysis; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects*; D017705:Lignans; D010042:Ouabain / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D011188:Potassium / Q000097:blood*; D011863:Radioimmunoassay; D012964:Sodium / Q000097:blood*
D007473:Ion Channels; D017705:Lignans; D010936:Plant Extracts; D010042:Ouabain; D004077:Digoxin; D012964:Sodium; D011188:Potassium