Recognition of alloantigens and induction of experimental allergic encephalomyelitis by a murine encephalitogenic T cell clone.
In this communication we report a SJL/J (H-2s, Mlsc)-derived encephalitogenic T cell clone 4b.14a which has dual specificities for myelin basic protein/I-As and allogeneic H-2Ik gene products. Monoclonal antibodies specific for public class II major histocompatibility complex (MHC) determinants (Ia.17, I-Ak, r and Ia.7) and anti-L3T4 antibody inhibited the response of the clone 4b.14a to alloantigens, but a monoclonal antibody specific for a private determinant on I-Ak (Ia.2) did not inhibit the response. Although this clone proliferated in response to allogeneic spleen cells expressing H-2Ik determinants regardless of disparate Mlsa, b, c, d alleles, CBA/N cells (H-2k, Mlsnull) failed to stimulate the clone 4b.14a. These results suggest that recognition of allogeneic class II MHC molecules by this clone requires recognition with non-MHC gene products such as Mls. In addition, the clone 4b.14a stimulated by alloantigens could mediate clinical signs of experimental allergic encephalomyelitis in syngeneic recipients. However, interleukin 2 of rat spleen cell origin alone failed to activate the clone cells to make them encephalitogenic, though it could make them proliferate. The significance of these findings for T cell recognition and activation is discussed.
D000818:Animals; D002999:Clone Cells; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000209:etiology / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology; D006651:Histocytochemistry; D007116:Immunization, Passive; D007376:Interleukin-2 / Q000378:metabolism; D007519:Isoantigens / Q000032:analysis* / Q000276:immunology; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D008285:Major Histocompatibility Complex; D051379:Mice; D008815:Mice, Inbred Strains / Q000276:immunology; D004676:Myelin Basic Protein / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology* / Q000637:transplantation; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D007519:Isoantigens; D004676:Myelin Basic Protein