Expression of human chromosome 11-encoded cell-surface antigens by DNA-mediated transfectants.
DNA-mediated transfectants were isolated that expressed two of the cell-surface antigens encoded by human chromosome 11. These tranfectants were used to analyze monoclonal antibodies selected to recognize human cell-surface antigens expressed by a somatic cell hybrid containing 11 as its only human chromosome. Analysis of the transfectants, deletion hybrids, and mutants showed that the monoclonal antibodies recognized at least five different antigens, one of which we had not identified previously. A majority of the monoclonal antibodies recognized the a1 antigen. The use of cells from higher primates demonstrated that the a1-specific monoclonal antibodies recognize at least two epitopes.
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000235:genetics*; D002460:Cell Line; D002906:Chromosomes, Human, 6-12 and X; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics*; D000939:Epitopes / Q000032:analysis; D005260:Female; D015186:Hominidae; D006801:Humans; D006822:Hybrid Cells; D010053:Ovary; D013045:Species Specificity; D014162:Transfection
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000939:Epitopes; D004247:DNA