[Isolation and immunochemical characteristics of soluble membrane proteins from the cell wall of Corynebacterium diphtheria].
A method for isolation of immunochemically active proteins from Corynebacterium diphtheria membranes was elaborated. The proteins were solubilized with the nonionic detergent NP-40 and gel-filtered through an Ultrogel AcA-34 column under denaturating conditions. The purified proteins (Mr = 64 kD) were antigenically active in a solid phase radioimmunoassay with human antidiphtheria antibodies.
D000596:Amino Acids / Q000032:analysis; D000942:Antigens, Bacterial / Q000032:analysis; D002473:Cell Wall / Q000032:analysis; D002850:Chromatography, Gel; D003353:Corynebacterium diphtheriae / Q000032:analysis* / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D008565:Membrane Proteins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D012995:Solubility
D000596:Amino Acids; D000942:Antigens, Bacterial; D000939:Epitopes; D008565:Membrane Proteins; D009005:Monosaccharides
Vydelenie i immunokhimicheskaia kharakteristika rastvorimykh membrannykh belkov kletochnykh stenok korinebakteriĭ difterii.