Biochemical characterization of the adhesion-related differentiation antigen XT-1.
The XT-1-molecule, an adhesion-related differentiation antigen of male mouse germ cells, is a 34 000 Mr glycoprotein with major charge isomer at pI 5.1 and is an integral component of the cell membrane. On large late pachytene spermatocyte, the molecule is present at a concentration of 2.5 X 10(3) molecules micron-2, which approximates HLA/ABC concentration on lymphocytes. By comparing the reactivity of four anti-XT-1 monoclonal antibodies, three of which elicit germ cell-germ cell adhesion, we have defined two distinct surface regions of the XT-1-molecule. The relationship of the XT-1-molecule with other known adhesion-related molecules and testicular antigens is discussed.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis*; D002448:Cell Adhesion; D002454:Cell Differentiation; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D005854:Germ Cells / Q000276:immunology; D008297:Male; D051379:Mice; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000939:Epitopes