Toxic response of hamster embryo cells on exposure to mixtures of Ni2+ and benzo(a)pyrene.
Exposure of hamster embryo cells (HEC) to mixtures of benzo(a) pyrene and soluble Ni2+ neutral salts elicits additive responses in the enhanced nucleoside excretion assay. Ni2+ shows no significant excretion response until concentrations near 10 microM are reached. Addition of 0.4 micromolar benzo(a)pyrene and 30 microM, or 325 microM Ni2+ to HEC cultures results in total excretion equal to the sum of excretions induced by the individual chemicals. These observations suggest that excretion of pyrimidines may be a useful measure of biological dosimetry.
D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D002478:Cells, Cultured; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian; D008647:Mesocricetus; D009532:Nickel / Q000633:toxicity*; D011743:Pyrimidines / Q000378:metabolism; D012313:RNA / Q000378:metabolism
D011743:Pyrimidines; D001564:Benzo(a)pyrene; D012313:RNA; D009532:Nickel