Neuronal pp60c-src contains a six-amino acid insertion relative to its non-neuronal counterpart.
Neuronal cells express a pp60c-src variant that displays an altered electrophoretic mobility and a different V8 peptide pattern relative to pp60c-src expressed in tissues of non-neuronal origin. To determine whether the neuronal form of pp60c-src is encoded by a brain-specific messenger RNA, a mouse brain complementary DNA (cDNA) library was screened with a chicken c-src probe and a 3.8-kilobase c-src cDNA clone was isolated. This clone encodes a 60-kilodalton protein that differs from chicken or human pp60c-src primarily in having six extra amino acids (Arg-Lys-Val-Asp-Val-Arg) within the NH2-terminal 16 kilodaltons of the molecule. S1 nuclease protection analysis confirmed that brain c-src RNA contains an 18-nucleotide insertion at the position of the extra six amino acids. This insertion occurs at a position that corresponds to a splice junction in the chicken and human c-src genes. The isolated c-src cDNA clone encodes a protein that displays an identical V8 peptide pattern to that observed in pp60c-src isolated from tissues of neuronal origin.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology; D002645:Chickens; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D051379:Mice; D009474:Neurons / Q000201:enzymology*; D011494:Protein Kinases / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016392:Proto-Oncogene Proteins pp60(c-src); D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D004251:DNA Transposable Elements; D007527:Isoenzymes; D011518:Proto-Oncogene Proteins; D004247:DNA; D011494:Protein Kinases; D016392:Proto-Oncogene Proteins pp60(c-src); D004262:DNA Restriction Enzymes