title: Idiotypic characteristics of lambda 1-bearing anti-alpha(1----3)dextran response in wild mice: high frequency of responder strains and co-expression of IdI 558 and IdI 104 on the same molecule.
abstract: In laboratory mouse strains, only mice with the IghCa phenotype are able to mount an immune response against the alpha(1----3) glycosidic linkage of the B1355 dextran. The response is highly restricted with respect to utilization of only V lambda 1 and few related members of the VH558 family, and characterized by the production of cross-reacting (IdX), and individual (IdI) 104 and 558 idiotopes. We report in this study data showing a large degree of polymorphism in the anti-alpha(1----3)Dex response among 35 wild-derived mouse strains belonging to 4 different species. In contrast to the anti-alpha(1----3)Dex response of common laboratory strains, neither this response nor expression of the IdX, and IdI 104 and 558 idiotopes are linked to the IghCa haplotype. We also demonstrate co-expression on the same molecule of IdI 104 and IdI 558 idiotopes in wild mouse-derived hybridomas. These data challenge the idea of the exclusive expression at the molecular level of IdI 104 or IdI 558.
mesh_terms: D000818:Animals; D000835:Animals, Wild / Q000276:immunology; D001666:Binding Sites, Antibody; D003911:Dextrans / Q000276:immunology*; D006825:Hybridomas; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007146:Immunoglobulin lambda-Chains / Q000276:immunology*; D051379:Mice / Q000276:immunology*; D009115:Muridae / Q000276:immunology*
chemical_list: D003911:Dextrans; D007130:Immunoglobulin Idiotypes; D007146:Immunoglobulin lambda-Chains