Synthesis of the growth hormone-regulated rat liver anti-protease GHR-P63 is inhibited by acute inflammation.
Synthesis of the growth hormone-regulated anti-protease GHR-P63 by rat hepatocytes was strongly reduced during acute inflammation. This decrease was detected 8 h after the onset of inflammation and reached a maximum after 24 h. A decrease in the GHR-P63 mRNA level measured by in vitro translation and by hybridization mainly accounted for the alteration of GHR-P63 synthesis. Besides this major pretranslational mechanism, inflammation also interfered with GHR-P63 synthesis at a posttranslational level. This was indicated by the production of abnormal immunoprecipitable species at early stages of the acute-phase response.
D000208:Acute Disease; D000209:Acute-Phase Proteins / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D013006:Growth Hormone / Q000502:physiology*; D066298:In Vitro Techniques; D007249:Inflammation; D007700:Kinetics; D008099:Liver / Q000378:metabolism / Q000503:physiopathology*; D008297:Male; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D015843:Serpins; D014425:Turpentine
D000209:Acute-Phase Proteins; D012333:RNA, Messenger; C050936:Serpina3c protein, rat; D015843:Serpins; D013006:Growth Hormone; D014425:Turpentine