Protein and nucleotide contamination of bovine liver catalase used in culture medium explains growth of Trypanosoma cruzi.
Commercially available bovine liver catalase has been used to supplement chemically defined medium for growth of Trypanosoma cruzi. The protein extract was found to be contaminated with 25 to 30 protein bands as well as DNA and RNA polymers.
D000818:Animals; D002374:Catalase / Q000378:metabolism*; D002417:Cattle; D003470:Culture Media / Q000032:analysis*; D004247:DNA / Q000378:metabolism; D004586:Electrophoresis; D008099:Liver / Q000201:enzymology; D011687:Purines / Q000096:biosynthesis; D012313:RNA / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000378:metabolism*
D003470:Culture Media; D011687:Purines; D012313:RNA; D004247:DNA; D002374:Catalase