Acute phase plasma proteins in kininogen-deficient Brown Norway rats.
We examined whether Brown Norway rat plasma (BN/May Pfd f) contains alpha 1-cysteine proteinase inhibitor (alpha 1-CPI), also called major acute phase alpha 1-protein or T-kininogen. T-kininogen is a low molecular weight kininogen from which kinin can be released by trypsin but not by kallikreins. The BN plasma reacted with rabbit anti-alpha 1-CPI gamma globulins. Purified alpha 1-CPI released a kinin-like activity with trypsin and with homogenate of salivary glands, as Brown Norway rat plasma did. High concentration of added rat urine induced a small release (10%) of kinin from alpha 1-CPI. Preincubation of Brown Norway rat plasma with rabbit anti-rat alpha 1-CPI gamma-globulins nearly suppressed the kinin-forming substrate of trypsin in this plasma. These results indicated that plasma of our Brown Norway rats contains only alpha 1-CPI as kinin-forming substrate. This plasma contains low amount of alpha 2-macroglobulin, while its content in orosomucoid and haptoglobin was a little larger than that of Wistar rat plasma.
D000209:Acute-Phase Proteins / Q000097:blood*; D000818:Animals; D001798:Blood Proteins; D001920:Bradykinin / Q000378:metabolism; D015853:Cysteine Proteinase Inhibitors; D005260:Female; D007122:Immunoelectrophoresis; D007704:Kininogens / Q000172:deficiency*; D007705:Kinins / Q000097:blood; D008297:Male; D008970:Molecular Weight; D051381:Rats; D011914:Rats, Inbred BN; D011919:Rats, Inbred Strains; D012469:Salivary Glands / Q000378:metabolism; D014357:Trypsin / Q000494:pharmacology; D014425:Turpentine / Q000494:pharmacology; D014556:Urine
D000209:Acute-Phase Proteins; D001798:Blood Proteins; D015853:Cysteine Proteinase Inhibitors; D007704:Kininogens; D007705:Kinins; D014357:Trypsin; D001920:Bradykinin; D014425:Turpentine