Rapid identification of Klebsiella pneumoniae in the lung tissue by fluorescence microscopy.
Klebsiella pneumoniae is stained weekly with hematoxylin and eosin, moderately with periodic acid Schiff (PAS), and strongly by the Warthin-Starry method. Staining by the Warthin-Starry method, however, takes more than one hour. Therefore, we developed a new procedure for rapid identification of K. pneumoniae in paraffin sections of the lung under a fluorescence microscope.
D000818:Animals; D007710:Klebsiella Infections / Q000175:diagnosis*; D007711:Klebsiella pneumoniae / Q000302:isolation & purification*; D051379:Mice; D008856:Microscopy, Fluorescence; D011014:Pneumonia / Q000175:diagnosis*; D013194:Staining and Labeling