[Levels of fraction I and VW antigens in cultures of the plague microbe grown on yeast-casein, yeast-Hottinger broth and yeast-sunflower oil cake media].
The content of fraction 1 and VW-antigens in Y. pestis cultures grown in different media (yeast-casein medium, yeast medium with Hottinger digest, and yeast medium with sunflower-seed protein) was studied over the course of their growth by means of the antibody neutralization and microprecipitation in agar tests. The media under study were not inferior to the casein sulfuric hydrolysate-based medium used for control in their capacity for ensuring the synthesis of VW-antigens. The maximum accumulation of fraction 1 was observed in yeast medium with sunflower-seed protein. In all media the maximum content of fraction 1 was registered on day 3 of cultivation, and the maximum accumulation of VW-antigens on days 8-9 of incubation at 37 degrees C. The data obtained in this study make it possible to regard fraction 1 and VW-antigens as the secondary metabolites of Y. pestis.
D000942:Antigens, Bacterial / Q000032:analysis*; D002364:Caseins; D003470:Culture Media / Q000378:metabolism*; D000939:Epitopes / Q000032:analysis; D006368:Helianthus; D052899:Pore Forming Cytotoxic Proteins; D011492:Protein Hydrolysates; D012639:Seeds; D013696:Temperature; D013997:Time Factors; D014774:Virulence; D015002:Yeast, Dried; D015010:Yersinia pestis / Q000254:growth & development / Q000276:immunology* / Q000472:pathogenicity
D000942:Antigens, Bacterial; D002364:Caseins; D003470:Culture Media; D000939:Epitopes; C043908:LcrV protein, Yersinia; D052899:Pore Forming Cytotoxic Proteins; D011492:Protein Hydrolysates
Soderzhanie fraktsii I i VW-antigenov v kul'turakh chumnogo mikroba, vyrashchennykh na sredakh DK, DKh i DP.