Adhesion and the cell cycle in cultured L929 and CHO cells.
The adhesiveness of L929 and CHO-K1 cells was monitored throughout their respective cell cycles. The cell cycle stages were identified by a combination of measuring, histochemical and autoradiographic techniques. Adhesiveness was shown to be maximal during the S and late G2 phases, and minimal at mitosis. S cells adhered selectively to other S cells, while late G2 phase cells adhered selectively to other late G2 cells. Mixing of the two cell lines, L929 and CHO-K1, resulted in the S phase cells adhering to each other irrespective of the cell line to which they belonged. The phases of increased adhesiveness and selectivity coincided with a decrease in cell surface negative charge, corresponding to well documented changes in the morphology of the cells.
D000818:Animals; D002448:Cell Adhesion; D002453:Cell Cycle; D002460:Cell Line; D004261:DNA Replication; D007700:Kinetics; D007739:L Cells / Q000166:cytology; D051379:Mice; D011796:Quinacrine; D013194:Staining and Labeling
D011796:Quinacrine