Ethanol-induced component of 45Ca2+ uptake in PC12 cells is sensitive to Ca2+ channel modulating drugs.
We compared the properties of depolarization-dependent 45Ca2+ uptake in PC12 cells grown with and without 200 mM ethanol for 6 days. Ethanol exposure increased 45Ca2+ uptake by 54%, but the ethanol-induced component of uptake retained properties of Ca2+ flux through voltage-dependent Ca2+ channels, including sensitivity to Ca2+ channel modulating drugs. Such drugs may therefore have a role in counteracting ionic events underlying ethanol dependence and withdrawal.
D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002460:Cell Line; D004110:Diltiazem / Q000494:pharmacology; D004347:Drug Interactions; D000431:Ethanol / Q000037:antagonists & inhibitors*; D005444:Flunarizine / Q000494:pharmacology; D007473:Ion Channels / Q000187:drug effects*; D009543:Nifedipine / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
D002121:Calcium Channel Blockers; D007473:Ion Channels; D000431:Ethanol; D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; D014700:Verapamil; D004110:Diltiazem; D009543:Nifedipine; D005444:Flunarizine; D002118:Calcium