In search of an in vitro index of viability during pancreatic preservation.
To determine an index of viability during pancreatic preservation, 15 dogs underwent segmental pancreatic autografting after 24 hr of pulsatile perfusion. These animals were divided into Group A (6) and Group B (9) on the basis of post-transplant normoglycemia or hyperglycemia. In each experiment, sequential perfusate amylase and arterial and venous blood gases were analyzed during preservation. In addition, sequential pancreatic tissue slices were obtained to determine in vitro insulin release. A comparison of perfusion parameters demonstrated no significant differences in pancreas weight (41 +/- 2.9 vs 38.3 +/- 1.5 mg), perfusate flow (0.24 +/- 0.2 vs 0.20 +/- 0.06 ml/min/g), diastolic (24.3 +/- 2.3 vs 26.9 +/- 1.6 mm Hg) or mean pressure (27.4 +/- 1.9 vs 30.1 +/- 1.5 mm Hg). Perfusate amylase levels (u/100 ml) and percentage change from baseline are as follows: (Table: see text). Perfusate amylase was significantly greater at 21 hr in Group A (P less than 0.005). In addition, a significantly greater rate of amylase release was evident in Group A at 1 hr (P less than 0.02), 3 hr (P less than 0.02), and 21 hr (P less than 0.01). Group B pancreata demonstrated significantly increased oxygen extraction at 1 hr (A-V O2 difference: Group A = -33, Group B = -68; P less than 0.05). In vitro insulin release of tissue slices obtained pre-harvest, post-flush, post-preservation, and 15 min post-transplantation was not significantly different in the two groups. In conclusion, sequential perfusate amylase and blood gas determinations may be useful in predicting pancreatic transplant function.
D000681:Amylases / Q000032:analysis / Q000097:blood; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000097:blood; D004285:Dogs; D005260:Female; D005951:Glucose Tolerance Test; D066298:In Vitro Techniques; D007328:Insulin / Q000032:analysis / Q000097:blood; D009926:Organ Preservation / Q000379:methods*; D010100:Oxygen / Q000097:blood; D016035:Pancreas Transplantation; D010477:Perfusion; D011379:Prognosis; D011863:Radioimmunoassay; D014022:Tissue Survival
D001786:Blood Glucose; D007328:Insulin; D000681:Amylases; D010100:Oxygen