Idiotypic analysis of five xenogeneic antisera to anti-human thyroglobulin monoclonal antibodies.
Five xenogeneic anti-idiotypic antisera (anti-id) were produced against individual BALB/c-derived monoclonal antibodies (mAb) which bound to different peptidic determinants on the human thyroglobulin molecule (Tg). Idiotypic analysis performed using sensitive radioimmunoassays revealed that: (1) the anti-id highly precipitated their homologous ligands; (2) two anti-id displayed minor cross-reactivities with one or two heterologous mAb; (3) each unlabelled homologous mAb was able to inhibit the idiotype binding of the corresponding anti-id; (4) no significant inhibition of homologous idiotype binding was observed with large excess of heterologous mAb; (5) efficient inhibition of mAb binding to Tg was observed only when homologous anti-id served as inhibitor. The data support the conclusion that xenogeneic anti-id may detect on their corresponding ligands individual idiotypic specificities that can be located at the mAb-combining site. Such reagents may constitute appropriate probes for further studies on anti-Tg autoimmunity.
D000818:Animals; D000910:Antibodies, Heterophile / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001323:Autoantibodies / Q000032:analysis / Q000276:immunology*; D001665:Binding Sites; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000032:analysis* / Q000276:immunology; D007135:Immunoglobulin Variable Region / Q000032:analysis / Q000276:immunology; D007457:Iodine Radioisotopes; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D011817:Rabbits; D011863:Radioimmunoassay
D000910:Antibodies, Heterophile; D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D007135:Immunoglobulin Variable Region; D007457:Iodine Radioisotopes; C006606:anti-thyroglobulin