Quantification of proteins in the low nanogram range by staining with the colloidal gold stain AuroDye.
A simple assay for proteins in the low nanogram range is described. Proteins are bound to nitrocellulose paper in a band, stained with the commercially available colloidal gold stain AuroDye (a registered trademark of Jansen Life Sciences Products), and quantified by scanning densitometry: 1.25 to 20 ng of protein can be consistently measured. Variation in the staining intensity of different proteins is discussed. DNA, RNA, polyvinylpyrrolidone, Ficoll, and 6 M urea do not affect the assays, and samples from cellular material in a simple lysis buffer containing 0.1% of sodium dodecyl sulfate can be assayed.
D000818:Animals; D003101:Collodion; D003720:Densitometry; D006046:Gold; D008832:Microchemistry; D010047:Ovalbumin / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis; D013194:Staining and Labeling / Q000379:methods*; D005719:gamma-Globulins / Q000032:analysis
D011506:Proteins; D005719:gamma-Globulins; D012710:Serum Albumin, Bovine; D006046:Gold; D003101:Collodion; D010047:Ovalbumin