Direct analysis of RNA in whole cell and cytoplasmic extracts by gel electrophoresis.
Procedures are presented by which whole cell, cytoplasmic, or nuclear extracts can be subjected to gel electrophoresis for the separation of the various RNA species, which are then analyzed by conventional blotting and hybridization techniques. Since the methods for preparing the extracts do not involve precipitation or two-phase extraction steps, the minimum number of cells that can be processed is limited only by the sensitivity of detection for specific RNA species. Multiple small or large aliquots of tissue culture cells can be quickly prepared. Cell preparations with high RNase levels, such as resting human lymphocytes or HL60, can be processed reliably with these procedures.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D002477:Cells / Q000032:analysis*; D002478:Cells, Cultured; D003593:Cytoplasm / Q000032:analysis*; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000032:analysis*
D012313:RNA