Vasoactive intestinal peptide (VIP) induces a K+ current in the Xenopus oocyte membrane.
Evidence, based on voltage-clamp and current-clamp recording, is presented for endogenous receptors for vasoactive intestinal polypeptide (VIP) on the cell membrane of the Xenopus oocyte. At normal resting potential a hyperpolarisation is produced by VIP, associated with an increase in K+ conductance. The response is dose-dependent, with a threshold near 10(-9)M VIP. The effect is potentiated by forskolin. Of several other types of neuropeptide tested, only avian pancreatic polypeptide gives a response: this is similar to that of VIP, but this peptide can also potentiate the response to VIP.
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism; D005576:Colforsin / Q000494:pharmacology; D004789:Enzyme Activation; D007473:Ion Channels / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects; D009865:Oocytes / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D011188:Potassium / Q000378:metabolism*; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*; D014982:Xenopus laevis
D007473:Ion Channels; D005576:Colforsin; D014660:Vasoactive Intestinal Peptide; D000262:Adenylyl Cyclases; D011188:Potassium