Isolation and partial characterization of a monoclonal antibody specific for a naturally occurring double-stranded RNA.
A monoclonal antibody (Mab) against the double-stranded form of the cucumber mosaic virus-associated RNA 5, the sequence variant that induces the tomato necrosis disease (ds(n)CARNA 5), has been obtained. Specificity studies using a radioimmunoassay showed that the purified Mab recognizes neither DNA nor ssRNA of different sources including ss(n)CARNA 5, poly(I).poly(C), nor dsRNA of two other sequence variants of CARNA 5. Therefore, the Mab seems to recognize a conformational variation only present in the double helical structure of ds(n)CARNA 5.
D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D000939:Epitopes; D009029:Mosaic Viruses / Q000276:immunology*; D009690:Nucleic Acid Conformation; D012330:RNA, Double-Stranded / Q000276:immunology*; D012367:RNA, Viral / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D012330:RNA, Double-Stranded; D012367:RNA, Viral