Separation and characterisation of glycoproteins from normal, pregnancy, and acute inflammatory sera.
A system of multiple chromatography combining anion-exchange, gel filtration, and affinity chromatography has been devised to separate several acute phase proteins from serum, including alpha 1 acid glycoprotein, transferrin, alpha 1-antichymotrypsin, alpha 1-antitrypsin and haemopexin. Only 5-15 ml of blood is required to provide milligram quantities of the purified proteins for further biochemical analysis. The system has been applied in a study of pregnancy and some diseases in order to investigate the changes in the serum protein profile and in individual proteins.
D000209:Acute-Phase Proteins / Q000097:blood* / Q000302:isolation & purification; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006023:Glycoproteins / Q000097:blood* / Q000302:isolation & purification; D006801:Humans; D005779:Immunodiffusion; D008970:Molecular Weight; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000097:blood* / Q000302:isolation & purification
D000209:Acute-Phase Proteins; D006023:Glycoproteins; D011257:Pregnancy Proteins