Anion-exchange chromatography of normal and monoclonal serum immunoglobulins.
Anion-exchange chromatography was used for separations of the three main immunoglobulin classes in human sera. The material included normal sera as well as sera with monoclonal immunoglobulin components. The Mono Q anion-exchange column was eluted with a stepwise gradient formed by 0.05 and 0.3 M phosphate buffers. No indications of negative effects on the sample components were observed and the protein recovery was high. A fast, easy and reproducible separation of immunoglobulins G, A and M was obtained.
D000911:Antibodies, Monoclonal / Q000032:analysis*; D002021:Buffers; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis*; D007525:Isoelectric Focusing; D011506:Proteins / Q000032:analysis; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D002021:Buffers; D007136:Immunoglobulins; D011506:Proteins