Comparison of in vivo and in vitro methods for assessing the effects of bromobenzene on the hepatic-metabolizing enzyme system.
The effect of daily i.p. injections of 0, 0.05, 0.5 and 5.0 mmol/kg bromobenzene for 1 week on the activity of the hepatic drug-metabolizing enzyme system was measured in the rat by a model substrate assay employing lindane (gamma-hexachlorocyclohexane) and by a battery of in vitro enzyme assays. The data in this study indicated that repeated pretreatment with bromobenzene for 1 week stimulated a dose-related increase in phase I reactions while inducing phase II reactions at the high dose (5 mmol/kg bromobenzene). The in vivo and in vitro assays showed good agreement.
D000818:Animals; D001711:Biotransformation; D001969:Bromobenzenes / Q000008:administration & dosage / Q000633:toxicity*; D002250:Carbon Radioisotopes; D005260:Female; D001556:Hexachlorocyclohexane / Q000378:metabolism*; D007274:Injections, Intraperitoneal; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D051381:Rats; D011916:Rats, Inbred F344
D001969:Bromobenzenes; D002250:Carbon Radioisotopes; D001556:Hexachlorocyclohexane; C032036:bromobenzene