Reduction of EGF receptor synthesis by antisense RNA vectors.
An association has been observed between squamous cell carcinomas and elevated EGF receptor levels. The approach taken here to analyze this problem was to construct vectors which express antisense RNA for the EGF receptor. The plasmid vector used was derived from pSV2-neo, which is readily selectable. Three EGF receptor cDNA fragments, a 5'-, a middle and a 3'-region were tested for differences in efficiency as antisense RNA. The results suggest that the 5'-fragment was the most efficient.
D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D002460:Cell Line; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004815:Epidermal Growth Factor / Q000378:metabolism; D066246:ErbB Receptors / Q000096:biosynthesis* / Q000235:genetics; D012313:RNA / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection
D004274:DNA, Recombinant; D012333:RNA, Messenger; D004815:Epidermal Growth Factor; D012313:RNA; D004247:DNA; D066246:ErbB Receptors; D004262:DNA Restriction Enzymes