[Immunochemical characteristics of the subunits of cholera enterotoxin and of thermolabile Escherichia coli enterotoxins of various origins].
Antigenic determinants of subunits A and B of cholera enterotoxin (CT), heat-labile enterotoxin from the human E. coli strain (hLT) and heat-labile enterotoxin from the porcine E. coli strain (pLT) were analysed by Ouchterlony double gel-diffusion test against antisera to B subunits of three toxins and antisera to three holotoxins. The results have shown the existence of the following antigenic determinants: in subunits B-1. antigenic determinants, common for B subunits of all three enterotoxins-B(chp); 2. group antigenic determinants, common for B subunits of two toxins in the pair-B(ch), B(hp); 3. antigenic determinants, unique for B subunits of each CT, hLT, pLT-(B(c), B(h), B(p); in subunits A.-1. antigenic determinants, common for A subunits of all three enterotoxins-A.(chp); 2. group antigenic determinants, common for A subunits of two enterotoxins (hLT and pLT/-A(hp); 3. antigenic determinants, unique for A subunit of CT-A(c). On the basis of these results antigenic formulas for subunits of CT, hLT, pLT were proposed.
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D001427:Bacterial Toxins / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D002772:Cholera Toxin / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D000939:Epitopes / Q000032:analysis; D004926:Escherichia coli / Q000302:isolation & purification; D029968:Escherichia coli Proteins; D006801:Humans; D007120:Immunochemistry; D005779:Immunodiffusion; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D013552:Swine / Q000382:microbiology
D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D004768:Enterotoxins; D000939:Epitopes; D029968:Escherichia coli Proteins; D010446:Peptide Fragments; D002772:Cholera Toxin; C027538:heat-labile enterotoxin, E coli
Immunokhimicheskie osobennosti sub''edinits kholernogo énterotoksina i termolabil'nykh énterotoksinov kishechnoĭ palochki razlichnogo proiskhozhdeniia.