Biochemical and epitope analysis of the 17-1A membrane antigen.
The antigen recognized by the monoclonal anti-colon carcinoma antibody 17-1A was found to be a 37 kD glycoprotein in three human colon carcinoma cell lines. The 17-1A antigen (17-1A Ag) contains asparagine-linked carbohydrates as determined by inhibition of glycosylation with tunicamycin. After reduction of 17-1A precipitates with 2-mercaptoethanol two bands of 33 and 40 kD were obtained from the cell lines DLD-1 and HT-29, but only one band from cell line WiDr. Four new MAbs generated against fresh colon carcinoma tissue were shown to be directed against the 17-1A Ag by immunoprecipitation and Western blotting. Two of these MAbs appear to define a novel epitope on the 17-1A Ag as judged from cross-blocking experiments and idiotype expression. Immunohistochemical analysis revealed that the 17-1A Ag is distinctly expressed on a variety of normal and malignant epithelial tissues.
D000936:Antigen-Antibody Complex / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000276:immunology*; D000954:Antigens, Surface / Q000032:analysis*; D002460:Cell Line; D003110:Colonic Neoplasms / Q000276:immunology*; D000939:Epitopes / Q000032:analysis*; D005434:Flow Cytometry; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D007413:Intestinal Mucosa / Q000276:immunology; D007668:Kidney / Q000276:immunology; D014018:Tissue Distribution
D000936:Antigen-Antibody Complex; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D000939:Epitopes; D006023:Glycoproteins