title: Further studies on murine macrophage synthesis of nitrite and nitrate.
abstract: Further studies on macrophage synthesis of nitrite and nitrate showed lipopolysaccharide (LPS) and interferon (IFN) to be potent stimuli. Kinetic experiments showed a time lag of 6 h for LPS and 10-12 h for IFN. The protein synthesis inhibitor cycloheximide completely inhibited nitrite and nitrate synthesis when present in the media at time 0 but had no effect if added at times after the lag period. A number of experiments were carried out to test the involvement of reactive oxygen species (the 'oxygen burst') in stimulated macrophage synthesis. All results were consistent with a lack of involvement of the oxygen burst in this process.
mesh_terms: D000818:Animals; D001711:Biotransformation; D007372:Interferons / Q000494:pharmacology; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D009566:Nitrates / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism*; D010101:Oxygen Consumption; D013997:Time Factors
chemical_list: D008070:Lipopolysaccharides; D009566:Nitrates; D009573:Nitrites; D007372:Interferons