Generation of competent virus in the REV helper cell line C3.
We have studied the generation of replication-competent virus in cultures of the helper cell line C3, which harbors a packaging-defective provirus derived from reticuloendotheliosis virus. We transfected the C3 line with a defective provirus encoding the chicken growth hormone gene and carrying the HSV-1 tk gene. The appearance of competent virus was assayed by infection of Spafas C/E CEF, monitoring reverse transcriptase activity, and rescue of the TKTU assayed on BRLtk- cells. Our results indicate that cultures of C3 producing TKTU can release viruses which have variable growth characteristics and which can remain latent in culture for extended periods of time.
D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D002999:Clone Cells / Q000382:microbiology*; D003673:Defective Viruses / Q000235:genetics / Q000502:physiology*; D005822:Genetic Vectors; D006378:Helper Viruses / Q000235:genetics / Q000502:physiology*; D006801:Humans; D012367:RNA, Viral / Q000032:analysis; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D011994:Recombinant Proteins / Q000032:analysis; D011995:Recombination, Genetic; D001356:Reticuloendotheliosis virus / Q000235:genetics / Q000502:physiology*; D012190:Retroviridae / Q000502:physiology*; D014764:Viral Proteins / Q000032:analysis; D014776:Virus Cultivation; D014779:Virus Replication
D012367:RNA, Viral; D011994:Recombinant Proteins; D014764:Viral Proteins; D012194:RNA-Directed DNA Polymerase