Sensitivity of protein and RNA synthesis to emetine in axenic Entamoeba histolytica.
The action of emetine on the incorporation of (14C)1-DL-valine and (14C)-8-adenine in TCA insoluble fraction of axenic Entamoeba histolytica was investigated. These compounds were almost exclusively incorporated in the 'protein' and 'RNA' fractions, respectively. Incorporation of the purine showed a significantly higher sensitivity to emetine. While the percentage inhibition of valine incorporation by emetine decreased with increasing pH, the susceptibility of adenine incorporation was greater in the alkaline range.
D000225:Adenine / Q000378:metabolism; D000818:Animals; D003470:Culture Media; D004640:Emetine / Q000494:pharmacology*; D004748:Entamoeba histolytica / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*; D014238:Trichloroacetic Acid; D014633:Valine / Q000378:metabolism
D003470:Culture Media; D014238:Trichloroacetic Acid; D012313:RNA; D014633:Valine; D000225:Adenine; D004640:Emetine