title: Regulation of single sodium channels in renal tissue: a role in sodium homeostasis.
abstract: The kidney is responsible for the maintenance of an organism's body solute and water balance (i.e., Na+ homeostasis). The distal nephron and the cortical collecting duct (CCD) (an example of a tight epithelium) are important sites of regulatory control over the rate of Na+ reabsorption. The Na+ channel, a specialized protein located in the apical membrane of CCD cells, is the specific site of transepithelial Na+ movement. Na+ entry into the cell across the apical membrane occurs by passive diffusion of Na+ down an electrochemical gradient. We have used the patch-voltage clamp method to examine single-channel conductance events of the amiloride-sensitive apical Na+ channel in A6 cells, a model of CCD. Two types of Na+ channel were identified. One type was characterized by low selectivity (Na+ to K+) and high conductance, the other by high selectivity and low conductance. The type and frequency of channel observed depended on the transporting state of the epithelium. In a tissue with poor transport rates, the low-selectivity type of channel was prevalent (the other type of channel was present, but in a very low density). Therefore, the poorly transporting tissue had an overall low apical Na+ conductance. In a tissue with high transport rates, the highly selective channel appeared to be predominant. In this case the net result was a highly Na+ conductive apical membrane.
mesh_terms: D001692:Biological Transport; D002460:Cell Line; D004848:Epithelium / Q000378:metabolism; D006706:Homeostasis; D007473:Ion Channels / Q000502:physiology*; D007668:Kidney / Q000502:physiology*; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism*
chemical_list: D007473:Ion Channels; D012964:Sodium; D011188:Potassium