title: Internal anion binding site and membrane potential dominate the regulation of proton pumping by the chromaffin granule ATPase.
abstract: Effects of anions and membrane potential on the reconstituted proton pump from chromaffin granules were investigated. When acetate was present inside of the vesicles, ATP-dependent proton uptake was absolutely dependent on external chloride. Without external chloride, however, substantial proton uptake was observed when chloride or sulfate was present inside of the vesicles. Inside negative membrane potential drove ATP-dependent proton uptake regardless of the anion species present inside or outside of the vesicles. It is concluded that the internal anion binding site and membrane potential regulate the proton pumping activity of the ATPase.
mesh_terms: D000085:Acetates / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D000838:Anions; D001665:Binding Sites; D002417:Cattle; D002712:Chlorides / Q000494:pharmacology; D002837:Chromaffin Granules / Q000201:enzymology*; D002838:Chromaffin System / Q000201:enzymology*; D007425:Intracellular Membranes / Q000201:enzymology; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D008564:Membrane Potentials; D010710:Phosphates / Q000494:pharmacology; D006180:Proton-Translocating ATPases / Q000378:metabolism*; D011522:Protons; D013431:Sulfates / Q000494:pharmacology
chemical_list: D000085:Acetates; D000838:Anions; D002712:Chlorides; D007473:Ion Channels; D010710:Phosphates; D011522:Protons; D013431:Sulfates; D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases