Paper sections of macroenzymatically delineated myocardial necrosis.
Until now, the disadvantages of the nitroblue tetrazolium method of detecting myocardial necrosis have been that the colour reaction tended to fade and the wet heart slices were inconvenient for demonstration and storage. We have therefore modified the rapid technique of mounting sections of whole organs on paper to preserve the colour reaction. We can now show the extent of necrosis in permanent, readily portable, paper-mounted heart sections within 24 hours (although 48 hours is more convenient). Multiple preparations from multiple slices can be prepared for clinicopathological correlation, comparison with other cases, teaching, presentation at meetings, use in the case notes and distribution with the postmortem report.
D000328:Adult; D006801:Humans; D008297:Male; D008867:Microtomy / Q000379:methods*; D008875:Middle Aged; D009206:Myocardium / Q000473:pathology*; D009336:Necrosis; D009580:Nitroblue Tetrazolium; D010209:Paper; D013194:Staining and Labeling
D009580:Nitroblue Tetrazolium