Effects of phospholipases C and D on ordering of channel proteins in the mitochondrial outer membrane.
The effects of phospholipases C and D on the state of order of the channels in the outer membranes of Neurospora mitochondria have been investigated by negative-stain electron microscopy and optical diffraction. Unlike the situation with phospholipase A2, treatment of the isolated membranes with phospholipase C or D does not induce crystallization of the channels in the membrane plane. Furthermore, treatment of already-formed periodic arrays of outer membrane channels with either phospholipase C or D causes loss of long-range order in the arrays. The latter result suggests that zwitterionic phospholipids may play an important role in stabilizing the periodic arrays of the channel-forming protein in this membrane.
D003460:Crystallization; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism*; D007473:Ion Channels / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D008854:Microscopy, Electron; D008928:Mitochondria / Q000648:ultrastructure*; D009491:Neurospora / Q000648:ultrastructure; D010739:Phospholipase D / Q000378:metabolism*; D010740:Phospholipases / Q000378:metabolism*; D010738:Type C Phospholipases / Q000378:metabolism*
D007473:Ion Channels; D008565:Membrane Proteins; D010740:Phospholipases; D010738:Type C Phospholipases; D010739:Phospholipase D