[Antigenicity of the auricular cartilage and the influence of vital preservation. 2: Identification of antigens of the auricular cartilage: animal experiment studies in rats].
We could demonstrate the existence of class I and class II antigens on the chondrocytes of the auricular cartilage in inbred strain rats (Louvain/M/Him, Fischer 344). Indirect fluorescence and related techniques were used. The cartilage was stored in Ham's nutrient medium F 12 or in the tissue culture for 8-10 days before allotransplantation. A complete disappearance of the antigens could not be achieved by means of the vital conservation. Grafting did not modify further the distribution of the antigens; in 2 experiments only renewed strong coloration of the chondrocyte-membranes and the surrounding matrix could be observed.
D000818:Animals; D046508:Culture Techniques; D004425:Ear Cartilage / Q000276:immunology / Q000637:transplantation*; D004431:Ear, External / Q000637:transplantation*; D000939:Epitopes; D005260:Female; D005455:Fluorescent Antibody Technique; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D008285:Major Histocompatibility Complex; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D011919:Rats, Inbred Strains; D014021:Tissue Preservation / Q000379:methods*
D000939:Epitopes; D000949:Histocompatibility Antigens Class II
Die Antigenität des Ohrknorpels und die Beeinflussung durch vitale Konservierung. Teil 2: Nachweis von Antigenen des Ohrknorpels: Tierexperimentelle Untersuchung an Ratten.