Localization of the cellular-fibronectin-specific epitope recognized by the monoclonal antibody IST-9 using fusion proteins expressed in E. coli.
Here we report on a monoclonal antibody (IST-9) which distinguishes between human cellular and plasma fibronectin. Using beta-galactosidase-fibronectin fusion proteins expressed in E. coli we have demonstrated that this monoclonal antibody is specific for a fibronectin segment (ED) which can be included or omitted from the molecule depending on the pattern of splicing of the mRNA precursors. Furthermore, using the same fusion proteins we have been able to localize precisely the epitopes of two other monoclonal antibodies (IST-1 and IST-2), specific for the heparin-binding domain 5 of fibronectin.
D000911:Antibodies, Monoclonal; D000939:Epitopes / Q000032:analysis*; D004926:Escherichia coli / Q000378:metabolism; D005353:Fibronectins / Q000032:analysis* / Q000276:immunology; D006801:Humans; D012326:RNA Splicing; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000939:Epitopes; D005353:Fibronectins; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D001616:beta-Galactosidase