Mapping of monoclonal antibodies to the Sendai virus P protein and the location of its phosphates.
The epitopes of a panel of five monoclonal antibodies to the Sendai virus P protein were mapped by generating modified forms of the P mRNA via SP6 expression followed by in vitro translation. The epitopes were found to be clustered in the C-terminal region of the protein. Two epitopes were within the last 30 residues, two were within the next 65, and one was between residues 308 and 451 of this 568-residue-long protein. By a combination of partial proteolysis and Western immunoblotting with one of these antibodies, the sites at which phosphates are added in vitro by the virion-associated kinase were mapped to the second quarter of the molecule from the N terminus.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D003001:Cloning, Molecular; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000276:immunology; D000939:Epitopes; D008970:Molecular Weight; D010222:Parainfluenza Virus 1, Human / Q000276:immunology*; D010750:Phosphoproteins / Q000235:genetics / Q000276:immunology*; D010766:Phosphorylation; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; C055859:P protein, Sendai virus; D010750:Phosphoproteins; D014764:Viral Proteins; D012321:DNA-Directed RNA Polymerases