Quantitative studies on the Rh-antigen D. Effect of the C gene.
Using an automated antiglobulin consumption test and Scatchard's analysis, the authors measured the number of D antigen epitopes on each of 46 samples of washed red cells (RBC) that represented six different Rh phenotypes. A clear D dosage effect was observed only when C was missing from the Rh genome, which confirmed the previously recognized suppressive effect of C. Thus, RBC of the Rh phenotype R2R2 had almost twice as many D epitopes (mean, 30,300/cell) as RBC of the Rh phenotypes R2r and R0r (means, 17,200 and 16,500 respectively). The data indicated that C in trans and cis suppressed similar amounts of D epitopes.
D001331:Automation; D003298:Coombs Test / Q000295:instrumentation; D000939:Epitopes / Q000032:analysis; D004912:Erythrocytes / Q000276:immunology; D005069:Evaluation Studies as Topic; D006801:Humans; D007519:Isoantigens / Q000276:immunology; D010641:Phenotype; D012204:Rh-Hr Blood-Group System / Q000032:analysis* / Q000235:genetics*; D013489:Suppression, Genetic
D000939:Epitopes; D007519:Isoantigens; D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen