Immunohistochemical localization of keratin in the taste buds of rat vallate papillae.
Keratin detected with anti-human whole keratin serum, raised in rabbits by injection of the isolated human whole keratin, was used as a histologic marker to study the origin of the cells in taste buds. Rat vallate papillae, including surrounding tissue, were processed for indirect immunofluorescent staining of ketatin. In the taste buds, most basal cells and some of the elongated cells were immunoreactive with anti-keratin serum, showing that most taste-bud cells, if not all, originate from cells of epithelial origin.
D000818:Animals; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D007633:Keratins / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013650:Taste Buds / Q000032:analysis*
D007633:Keratins