title: Characterization of cDNA clones for the human c-yes gene.
abstract: Three c-yes cDNA clones were obtained from poly(A)+ RNA of human embryo fibroblasts. Sequence analysis of the clones showed that they contained inserts corresponding to nearly full-length human c-yes mRNA, which could encode a polypeptide of 543 amino acids with a relative molecular weight (Mr) of 60,801. The predicted amino acid sequence of the protein has no apparent membrane-spanning region or suspected ligand binding domain and closely resembles pp60c-src. Comparison of the sequences of c-yes and v-yes revealed that the v-yes gene contains most of the c-yes coding sequence except the region encoding its extreme carboxyl terminus. The region missing from the v-yes protein is the part that is highly conserved in cellular gene products of the protein-tyrosine kinase family.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D004622:Embryo, Mammalian; D004625:Embryo, Nonmammalian; D005796:Genes; D006801:Humans; D008970:Molecular Weight; D011061:Poly A / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000302:isolation & purification; D051575:Proto-Oncogene Proteins c-yes; D011519:Proto-Oncogenes; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D013045:Species Specificity; D019061:src-Family Kinases
chemical_list: D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA; D051575:Proto-Oncogene Proteins c-yes; D019061:src-Family Kinases