Inhibition of viral reverse transcriptase by 2',5'-oligoadenylates.
Viral reverse transcriptase activity was inhibited in a concentration dependent manner by 2',5'-oligoadenylate. Kinetically this inhibition was of a mixed type where 2',5'-oligoadenylate was not strictly competitive with dTTP. The potency of inhibition was more marked in the absence than in the presence of sulfhydryl agents. 2',5'-oligoadenylate had no effect on DNA-dependent E. coli DNA polymerase and was much less active against mammalian DNA polymerases. This is the first report of reverse transcriptase inhibition by an inducible constitutive natural ligand.
D000227:Adenine Nucleotides / Q000494:pharmacology*; D000818:Animals; D001354:Avian Leukosis Virus / Q000201:enzymology*; D009189:Avian Myeloblastosis Virus / Q000201:enzymology*; D001358:Avian Sarcoma Viruses / Q000201:enzymology*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D007700:Kinetics; D008979:Moloney murine leukemia virus / Q000201:enzymology*; D009843:Oligoribonucleotides / Q000494:pharmacology*; D018894:Reverse Transcriptase Inhibitors; D013045:Species Specificity
D000227:Adenine Nucleotides; D009843:Oligoribonucleotides; D018894:Reverse Transcriptase Inhibitors; C023505:2',5'-oligoadenylate; D004259:DNA-Directed DNA Polymerase