Computer prediction of potential immunogenic determinants from protein amino acid sequence.
In proteins, immunogenic determinants that can induce protein-reactive antipeptide antibodies reside mostly in those parts of the molecule that have a high tendency to form beta-turns. A program for an IBM personal computer which predicts protein immunogenic determinants is described. The program predicts potential immunogenic determinants from protein amino acid sequences according to a Chou-Fasman-based probability of a beta-turn occurrence, p greater than 1.5 X 10(-4)(P. Y. Chou and G. D. Fasman, 1978, Adv. Enzymol. 47, 46-148). Oncopeptides (whose efficacy in generating protein-reactive antipeptide antibodies has been described) with a beta-turn probability of p greater than 1.5 X 10(-4) elicited antipeptide antibodies that reacted with the parent oncoprotein at a rate of 96%, thus showing a surprisingly good correlation between the tendency to form a beta-turn and the protein reactivity of antipeptide antibodies. Potential immunogenic determinants were predicted on myohemerythrin and myoglobin.
D000595:Amino Acid Sequence; D003198:Computer Simulation; D000939:Epitopes / Q000032:analysis*; D006422:Hemerythrin / Q000031:analogs & derivatives / Q000032:analysis; D009211:Myoglobin / Q000032:analysis; D011506:Proteins / Q000032:analysis* / Q000276:immunology
D000939:Epitopes; D006422:Hemerythrin; D009211:Myoglobin; D011506:Proteins; C008571:myohemerythrin