title: Effect of modification of sulfhydryl groups in soybean beta-amylase on the interaction with substrate and inhibitors.
abstract: Methyl 2,4-dinitrophenyl disulfide (MDPS) is shown to be an effective methanethiolating reagent for sulfhydryl groups in proteins via thiol-disulfide exchange reaction. It reacts with the two reactive sulfhydryl groups (SH1 and SH2) in soybean beta-amylase. A decrease of the enzymatic activity accompanies the methanethiolation of SH2. After complete methanethiolation of SH2, the modified enzyme still has 9% of the initial activity. Modification of SH2 with cyanide and iodoacetamide reduces the enzymatic activity to 65 and 2% of the initial activity, respectively. Apparently, the residual activity depends upon the size of the substituent at SH2. The modified enzymes still have the almost same Km values for amylopectin and Kd values for enzyme-maltose and enzyme-cyclohexaamylose complexes as the native enzyme. In contrast to maltose and cyclohexaamylose, the Kd value of the enzyme-glucose complex increases in the order of cyanide-, MDPS-, and iodoacetamide-modified enzymes, indicating that SH2 is located near the binding site of glucose. It is proposed from the subsite structure of soybean beta-amylase that the position of SH2 and the glucose binding site is around subsite 1, where the nonreducing ends of the substrate bind productively.
mesh_terms: D000681:Amylases / Q000037:antagonists & inhibitors*; D003486:Cyanides / Q000494:pharmacology; D007460:Iodoacetamide / Q000494:pharmacology; D007700:Kinetics; D010944:Plants / Q000201:enzymology*; D013025:Glycine max; D013053:Spectrophotometry; D013438:Sulfhydryl Compounds; D001614:beta-Amylase / Q000037:antagonists & inhibitors*
chemical_list: D003486:Cyanides; D013438:Sulfhydryl Compounds; D000681:Amylases; D001614:beta-Amylase; D007460:Iodoacetamide