title: Schistosoma mansoni: immunogenic glycoproteins of the cercarial glycocalyx.
abstract: Immunochemical studies at the level of the light and electron microscope showed that a monoclonal antibody, 128C3/3, was directed to an epitope in the glycocalyx of Schistosoma mansoni cercariae. Immunoprecipitation of surface labeled cercarial extracts with this monoclonal antibody demonstrated that the glycocalyx is composed of at least five components, including a very large molecular size polypeptide and polypeptides of 220, 180, 170, and 15 kDa. After transformation of cercariae to schistosomula, these polypeptides were shed from the surface and were therefore no longer accessible to surface labeling. Monoclonal antibody 128C3/3 was also reactive with a 38 kDa polypeptide from schistosomula; this polypeptide was weakly expressed on the surface of cercariae. Analysis of immunoprecipitates of radioiodinated protein extracts of cercariae, newly transformed schistosomula, and 36 hr in vitro cultured schistosomula showed that the 180 and 170 kDa polypeptides continued to be expressed within the organism following transformation, but were not accessible to surface labeling. Lectin binding studies revealed differences in the oligosaccharide composition of the six polypeptides. With the exception of the 15 kDa antigen, all the polypeptides reactive with 128C3/3 were highly immunogenic in infected mice and humans.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000947:Antigens, Helminth / Q000032:analysis / Q000276:immunology*; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology*; D010455:Peptides / Q000032:analysis / Q000276:immunology; D011134:Polysaccharides / Q000032:analysis / Q000276:immunology*; D012550:Schistosoma mansoni / Q000254:growth & development / Q000276:immunology* / Q000648:ultrastructure
chemical_list: D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D000939:Epitopes; D006023:Glycoproteins; D010455:Peptides; D011134:Polysaccharides