The elimination of mycoplasma from infected hybridomas by passaging in BALB/c mice.
Hybridomas, which were found to be infected with mycoplasma, were cleared of contamination by passaging in BALB/c mice. This procedure was successfully applied to four of five cultures examined. The procedure offers a simple and effective means of eliminating mycoplasma from valuable and sometimes irreplaceable hybridoma cell cultures.
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001202:Ascitic Fluid / Q000378:metabolism / Q000382:microbiology; D001431:Bacteriological Techniques; D006690:Bisbenzimidazole; D002455:Cell Division; D006825:Hybridomas / Q000032:analysis / Q000276:immunology / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009174:Mycoplasma / Q000032:analysis / Q000254:growth & development* / Q000276:immunology; D013194:Staining and Labeling
D000907:Antibodies, Bacterial; D006690:Bisbenzimidazole