Small megakaryocytes--an identification problem.
The number of bone marrow megakaryocytes (MK) was estimated in May Grunwald-Giemsa-stained (MGG) and immunoenzymatically stained smears. The latter was performed after incubation with a monoclonal antibody (ITI-PL1) directed against blood platelets and megakaryocytes (MK). The MK were grouped according to their size (diameter above or below 30 micron, respectively). Altogether 7.9% more MK were recognized in ITI-PL1 stained smears as compared to MGG-stained smears, mainly because comparatively few small MK were recognized in MGG-stained smears.
D000911:Antibodies, Monoclonal; D001854:Bone Marrow Cells; D000939:Epitopes / Q000032:analysis; D006801:Humans; D008533:Megakaryocytes / Q000166:cytology*; D010980:Platelet Membrane Glycoproteins / Q000032:analysis; D012016:Reference Values; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D000939:Epitopes; D010980:Platelet Membrane Glycoproteins