Primary syngeneic sensitization on monolayers of thyroid epithelial cells. X. Inhibition of T-cell proliferative response by thyroglobulin-specific monoclonal antibodies.
We studied the biochemical properties and the reactivity of 13 monoclonal anti-thyroglobulin antibodies, obtained by fusing P3 X 63 (8653) myeloma cells with spleen cells from CBA and C57BL/6 mice previously immunized with syngeneic thyroglobulin. We demonstrated that among the 13 anti-thyroglobulin monoclonal antibodies that we studied, each of them was specific for only syngeneic thyroglobulin, as each of them was able to cross-react with heterologous or xenogeneic thyroglobulin. Two major kinds of monoclonal antibodies were obtained: the first ones are able to block in vitro primary syngeneic sensitization and, moreover, they mutually compete for thyroglobulin binding; the second ones are not involved in this in vitro PSS and do not compete for binding to thyroglobulin. These results suggest that a defined particular epitope, borne by thyroglobulin and expressed on the thyroid epithelial cells, is responsible for in vitro primary syngeneic sensitization.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001323:Autoantibodies / Q000276:immunology; D001667:Binding, Competitive; D003429:Cross Reactions; D004847:Epithelial Cells; D000939:Epitopes; D008213:Lymphocyte Activation; D051379:Mice; D008815:Mice, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology*; D013954:Thyroglobulin / Q000276:immunology; D013961:Thyroid Gland / Q000166:cytology*
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D000939:Epitopes; D013954:Thyroglobulin