Isolation of HIV from Israeli patients and asymptomatic carriers.
Human immunodeficiency virus (HIV 1) strains were isolated from peripheral blood lymphocytes of eight homosexual men (two asymptomatic and six with lymphadenopathy syndrome) and of the wife of an AIDS patient with AIDS-related complex. All except one had antibodies to HIV in their serum. Virus isolation was carried out by cocultivation of the donors' peripheral lymphocytes with HUT-78 cells or with phytohemagglutinin-stimulated normal cord blood lymphocytes. Identification of the virus was based on detection of specific cytopathogenic effects, determination of reverse transcriptase activity, specific immunofluorescence and Southern blot analysis of HIV proviral DNA in the infected cell cultures.
D000386:AIDS-Related Complex / Q000382:microbiology*; D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D000914:Antibodies, Viral / Q000032:analysis; D002353:Carrier State / Q000382:microbiology*; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D005260:Female; D006678:HIV / Q000201:enzymology / Q000276:immunology / Q000302:isolation & purification*; D015483:HIV Antibodies; D006679:HIV Seropositivity; D006716:Homosexuality; D006801:Humans; D007118:Immunoassay; D007557:Israel; D008297:Male; D009693:Nucleic Acid Hybridization; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism
D000914:Antibodies, Viral; D015483:HIV Antibodies; D012194:RNA-Directed DNA Polymerase