Studies of the endothelial origin of cells in systemic angioendotheliomatosis and other vascular lesions of the brain and meninges using ulex europaeus lectin stains.
Ulex europaeus agglutinin I (UEA-I) is a plant lectin which binds specifically to alpha-L-fucose moieties on the surface glycoproteins of human endothelial cells. The binding is completely inhibited by preincubation of the lectin with fucose. UEA-I can be conjugated directly to fluorescein or peroxidase and can be used to stain endothelium of paraffin embedded tissues. UEA-I staining was evaluated on normal and infarcted brain, systemic angioendotheliomatosis, metastatic epidural angiosarcoma, hemangioendothelioma, hemangioblastoma, angioblastic meningioma of both the hemangioblastic and hemangiopericytic types, and vascular meningioma. The endothelium, but not neuropil of normal and infarcted brain was positive for UEA-I. The tumor cells of hemangioendothelioma and angiosarcoma also stained. However, no staining was seen in malignant intravascular cells of angioendotheliomatosis, the stromal cells of hemangioblastoma, or pericytes of angioblastic meningioma. It is concluded that the malignant cells in angioendotheliomatosis, the stromal cells of hemangioblastoma and the pericytes of angioblastic meningioma do not produce surface glycoproteins characteristic of endothelial cells.
D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D002196:Capillaries / Q000166:cytology; D004727:Endothelium / Q000378:metabolism; D005643:Fucose / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D006390:Hemangioendothelioma / Q000378:metabolism / Q000473:pathology*; D006393:Hemangiopericytoma / Q000378:metabolism / Q000473:pathology; D006394:Hemangiosarcoma / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D008579:Meningioma / Q000378:metabolism / Q000473:pathology*; D037121:Plant Lectins; D013194:Staining and Labeling
D006023:Glycoproteins; D037102:Lectins; D037121:Plant Lectins; C036850:Ulex europaeus lectins; D005643:Fucose