Expression of OKM5 antigen on human keratinocytes in vitro upon stimulation with gamma-interferon.
Using murine monoclonal antibodies against human OKM5, OKM1 and HLA-DR antigens antigenic characteristics of freshly separated human epidermal cells (EC) and those of EC cultured in the presence of Interferon-gamma (IFN-gamma) were studied. After 8-12 days of culture, primarily OKM1- OKM5- HLA-DR- keratinocytes displayed OKM5 and HLA-DR antigens when exposed to IFN-gamma. Our data support the concept, that human keratinocytes may possess accessory cell functions.
D000954:Antigens, Surface / Q000032:analysis*; D002478:Cells, Cultured; D000078404:Epidermal Cells; D004817:Epidermis / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D007633:Keratins
D000954:Antigens, Surface; D006684:HLA-DR Antigens; D007633:Keratins; D007371:Interferon-gamma