Allorecognition of purified major histocompatibility complex glycoproteins by cytotoxic T lymphocytes.
To investigate how T cells recognize allogeneic class I proteins encoded by the major histocompatibility complex (MHC), we examined the human cytotoxic T lymphocytes (CTL) elicited in a mixed lymphocyte reaction against a lymphoblastoid B-cell line (JY) whose MHC-class I proteins are HLA-A2 and -B7. By panning the responding T cells on plates that were coated with purified HLA-A2, an essentially pure population of CD8+ anti-HLA-A2 CTL was isolated in a single step and established as a cell line designated A2p. In addition to lysing HLA-A2+ target cells, the A2p cells lysed HLA-A2- cells, including mouse cells (P815), when purified native HLA-A2 was attached to them, but not when denatured HLA-A2 was attached. Thus, contrary to the general rule that T cells recognize sequential antigenic determinants in denatured protein antigens, the alloreactive CTLs appear to recognize determinants that depend upon the native configuration of HLA-A2; however, the possibility that these T cells recognize a peptide adduct persistently associated with purified, soluble HLA-A2 has not been ruled out.
D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis; D002448:Cell Adhesion; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D008285:Major Histocompatibility Complex; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D011489:Protein Denaturation; D013602:T-Lymphocytes, Cytotoxic / Q000145:classification / Q000276:immunology*
D000943:Antigens, Differentiation; D000939:Epitopes; D006680:HLA Antigens; D008562:Membrane Glycoproteins