title: Multiple tachykinin pools in sensory nerve fibres in the rabbit iris.
abstract: A population of sensory nerve fibres in the rabbit iris is known to contain calcitonin gene-related peptide and tachykinins, such as substance P and neurokinin A. In the presence of atropine and guanethidine, the isolated iris sphincter responded to electrical stimulation with a contraction that could be abolished by tachykinin antagonists. Capsaicin, known to release tachykinins from sensory fibres, evoked a long-lasting tachykinin-mediated contraction of the iris sphincter. Repeated application of capsaicin led to tachyphylaxis, possibly reflecting depletion of releasable neuronal stores of tachykinins. At this stage, electrical stimulation failed to elicit contraction. The capacity of capsaicin to release neuropeptides from sensory fibres was confirmed by determination of substance P- and calcitonin gene-related peptide-like immunoreactivity in the incubation medium and in the iris tissue. The concentrations of substance P and calcitonin gene-related peptide in the iris after capsaicin exposure were reduced by about 25%. Like capsaicin, bradykinin evoked a tachykinin-mediated contraction and tachyphylaxis. However, after development of tachyphylaxis to bradykinin, electrical stimulation or exposure to capsaicin still evoked tachykinin-mediated contraction, albeit a reduced one compared with the response before bradykinin. Hence, capsaicin completely depletes tachykinin stores releasable by prolonged electrical stimulation, whereas bradykinin exhausts only a sequestered pool. The possibility that tachykinins occur in several releasable pools in sensory nerves was investigated in yet another way: the iris sphincter muscle was stimulated electrically once every 2.5 min over several hours. The contractile response diminished gradually.(ABSTRACT TRUNCATED AT 250 WORDS)
mesh_terms: D000818:Animals; D001920:Bradykinin / Q000494:pharmacology*; D015740:Calcitonin Gene-Related Peptide; D002211:Capsaicin / Q000494:pharmacology*; D004558:Electric Stimulation; D005260:Female; D007498:Iris / Q000187:drug effects / Q000294:innervation*; D008297:Male; D009411:Nerve Endings / Q000187:drug effects*; D009475:Neurons, Afferent / Q000187:drug effects; D009479:Neuropeptides / Q000378:metabolism / Q000502:physiology*; D011817:Rabbits; D013373:Substance P / Q000378:metabolism; D015320:Tachykinins; D013618:Tachyphylaxis
chemical_list: D009479:Neuropeptides; D015320:Tachykinins; D013373:Substance P; D015740:Calcitonin Gene-Related Peptide; D002211:Capsaicin; D001920:Bradykinin