Characterization of monoclonal antibodies CBL3 and TT62 with differing antigenic determinants specific for isolated human islets.
Identification, separation and purification of human islets from acinar cells following collagenase digestion of the pancreas remains a difficult problem. We have identified and characterized two monoclonal antibodies, CBL3 and TT62, with specific binding activity to human islets but not acinar cells. The antigens these monoclonal antibodies recognize are lipid in nature and thus unaffected by collagenase digestion of the human pancreas. These properties allow isolated human islets to be identified by fluorescein isothiocyanate-labeled CBL3 and TT62, making these monoclonal antibodies potentially useful reagents in islet identification and purification.
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000276:immunology; D002469:Cell Separation / Q000379:methods; D002470:Cell Survival; D000939:Epitopes / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010179:Pancreas / Q000166:cytology / Q000276:immunology
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000939:Epitopes