Ethylnitrosourea treatment increases lectin binding to mouse germ cells.
Germ cell toxicity was assessed by investigating the binding of FITC-labeled lectins to mouse testis cells before and 18 days after treatment with ethylnitrosourea (ENU). Flow cytometry of testis cells dual-labeled with FITC-lectin plus the DNA stain, propidium iodide, allowed analysis of haploid (1C), diploid (2C), and dividing (4C) cell populations. Soybean agglutinin, wheat germ agglutinin, concanavalin A and Limax flavus agglutinin bound to normal mouse testis cells containing 1C, 2C or 4C DNA. Asparagus pea lectin and Bandeireae simplicifolia I isolectin B4 did not. ENU treatment reduced the number of testis cells and increased lectin binding, particularly of those lectins which bound to untreated cells.
D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003208:Concanavalin A / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D005038:Ethylnitrosourea / Q000633:toxicity*; D005434:Flow Cytometry; D037102:Lectins / Q000378:metabolism*; D008297:Male; D051379:Mice; D037121:Plant Lectins; D030262:Soybean Proteins; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling; D013737:Testis / Q000378:metabolism*; D014909:Wheat Germ Agglutinins / Q000378:metabolism; D010835:Phytohemagglutinins
D003208:Concanavalin A; D004247:DNA; D005038:Ethylnitrosourea; D037102:Lectins; D037121:Plant Lectins; D030262:Soybean Proteins; D014909:Wheat Germ Agglutinins; C028824:soybean lectin; D010835:Phytohemagglutinins