Immunoblot analysis of Salmonella typhi lipopolysaccharide (LPS) using typhoid sera.
Lipopolysaccharide (LPS) of Salmonella typhi has been analyzed by immunoblotting with pooled sera from typhoid patients. Pooled typhoid sera have recognized all the antigenic determinants of S. typhi LPS, giving a strong reaction with the repeating units on the O-side chains as well as with the core region. Cross-reacting antigens have been observed with the LPS of S. typhimurium and S. enteritidis, while no heterologous reactions were seen with the LPS of E. coli strains.
D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D004926:Escherichia coli / Q000276:immunology; D006801:Humans; D007158:Immunologic Techniques; D008070:Lipopolysaccharides / Q000032:analysis*; D012477:Salmonella enteritidis / Q000276:immunology; D012485:Salmonella typhi / Q000032:analysis* / Q000276:immunology; D014435:Typhoid Fever / Q000382:microbiology*
D000939:Epitopes; D008070:Lipopolysaccharides