In situ detection of enzymes in yeast.
A very simple method that allows for the rapid in situ assay of enzyme activity in yeast is described. Single colonies are collected on sticks (or glass micropipets), or multiple colonies are collected on sandpaper, and crushed onto nitrocellulose filters. The filters in turn are stained for the enzyme of interest using a histochemical assay. The method is quantitative and was found to work well for four enzymes in yeast.
D000135:Acid Phosphatase / Q000032:analysis*; D000469:Alkaline Phosphatase / Q000032:analysis*; D003101:Collodion; D005696:Galactosidases / Q000032:analysis*; D006651:Histocytochemistry; D011312:Pressure; D013194:Staining and Labeling; D014667:Vasopressins / Q000032:analysis*; D015003:Yeasts / Q000201:enzymology*; D001616:beta-Galactosidase / Q000032:analysis*
D014667:Vasopressins; D003101:Collodion; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D005696:Galactosidases; D001616:beta-Galactosidase