title: Monoclonal antibodies and characterization of epitopes of smooth Brucella lipopolysaccharides.
abstract: Four mouse monoclonal antibodies generated against Brucella melitensis 16M, and three generated against B. suis 1330 were analysed. An ELISA (enzyme-linked-immunosorbent assay) with whole cells as antigens was used to determine cross-reactivities with other Gram-negative bacteria. Two antibodies showed cross-reactivity with smooth Brucella strains only. Five antibodies also reacted with Yersinia enterocolitica O:9, but not with other bacteria. Two of these antibodies had significantly higher titres with A greater than M serotype Brucella strains, indicating that these epitopes are related to the antigenic A complex. The antigenic determinants recognized by the monoclonal antibodies showed varying degrees of susceptibility towards oxidation. They were shown by immunoblotting to be located on the polysaccharide moiety of the O-side chain.
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D002002:Brucella / Q000276:immunology*; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D006090:Gram-Negative Bacteria / Q000276:immunology; D006825:Hybridomas; D007118:Immunoassay; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D015008:Yersinia enterocolitica / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000939:Epitopes; D008070:Lipopolysaccharides