Voltage dependence of the actions of nifedipine and BAY K 8644 on barium currents recorded from single smooth muscle cells from the rabbit ear artery.
The voltage dependence of the actions of nifedipine and BAY K 8644 were studied on whole-cell barium currents recorded from single, collagenase- and elastase- dispersed, smooth muscle cells from the rabbit ear artery using standard patch clamp techniques (Hamill et al., 1981). The results showed that nifedipine (3 x 10(-7)M) reduced the inward current in response to depolarizations positive of -10mV but in the threshold range, -40mV to -20mV, that it increased the amplitude of the peak barium current. Subsequent application of BAY K 8644 (1.5 x 10(-7)M) enhanced this latter effect but decreased the inhibition induced by nifedipine at more positive potentials.
D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / Q000494:pharmacology*; D000818:Animals; D001464:Barium / Q000378:metabolism*; D005071:Evoked Potentials / Q000187:drug effects; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000187:drug effects* / Q000378:metabolism; D009543:Nifedipine / Q000494:pharmacology*; D011817:Rabbits
D007473:Ion Channels; D001464:Barium; D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; D009543:Nifedipine