title: Application of coomassie brilliant blue staining to cultured hepatocytes.
abstract: Coomassie brilliant blue staining developed by Pena (1980) was applied to cultured hepatocytes of adult rats with some modifications. Many of organelles in the cytoplasms were clearly visible as blue granules by this method. Various cytoskeletal elements were also visualized clearly. Because of its simplicity, Coomassie blue staining proved to be a very powerful tool for study of morphological changes of cell organelles and cytoskeletal systems of cultured hepatocytes.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000648:ultrastructure; D008099:Liver / Q000166:cytology*; D009940:Organoids / Q000648:ultrastructure; D051381:Rats; D012394:Rosaniline Dyes; D013194:Staining and Labeling; D013997:Time Factors
chemical_list: D012394:Rosaniline Dyes; C004692:coomassie Brilliant Blue