Neuronotoxicity of axonally transported toxic lectins, abrin, modeccin and volkensin in rat peripheral nervous system.
In an attempt to find new and more useful suicide transport agents, the cytotoxic lectins abrin, modeccin and volkensin were pressure microinjected into peripheral nerves (vagus, hypoglossal and sciatic) of adult rats. After 33 h-5 days survival, the brainstems, spinal cords and corresponding sensory ganglia were examined histologically. All three lectins produced profound chromatolysis, and destruction of sensory and motor neurons projecting axons through the injected nerves. Volkensin and modeccin were significantly more potent than any previously reported suicide transport agent. It is concluded that abrin, modeccin and volkensin are effective, unselective suicide transport agents in the rat peripheral nervous system but none is clearly superior to ricin for making restricted sensory and motor neuron ablations. However, modeccin and volkensin are fundamentally different from any previously reported suicide transport agents with respect to spread within the CNS which destroyed neurons adjacent to those initially taking up and transporting the toxin. Possibly this is due to the different oligosaccharide binding specificity of modeccin and volkensin compared to other suicide transport agents. Modeccin and/or volkensin may prove useful in making lesions of CNS interneurons using the suicide transport strategy.
D000036:Abrin / Q000633:toxicity*; D000818:Animals; D001370:Axonal Transport; D001933:Brain Stem / Q000187:drug effects / Q000473:pathology; D002490:Central Nervous System / Q000187:drug effects* / Q000473:pathology; D005727:Ganglia, Spinal / Q000187:drug effects / Q000378:metabolism; D006023:Glycoproteins; D037102:Lectins / Q000633:toxicity*; D008297:Male; D008845:Microinjections / Q000379:methods; D009699:N-Glycosyl Hydrolases; D010525:Peripheral Nerves / Q000187:drug effects* / Q000473:pathology; D037121:Plant Lectins; D010940:Plant Proteins / Q000633:toxicity*; D051381:Rats; D011919:Rats, Inbred Strains; D054790:Ribosome Inactivating Proteins, Type 2; D013116:Spinal Cord / Q000187:drug effects / Q000473:pathology
D006023:Glycoproteins; D037102:Lectins; D037121:Plant Lectins; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D000036:Abrin; C016004:modeccin; C049134:volkensin; D009699:N-Glycosyl Hydrolases