Neutralizing activity of monoclonal antibodies to heat-sensitive and heat-resistant epitopes of Rickettsia rickettsii surface proteins.
Antiprotein monoclonal antibodies derived from mice inoculated with Rickettsia rickettsii heated at 56 degrees C for 15 min are of two types: one is type specific for epitopes denatured by moderate temperatures, and the other is specific for epitopes resistant to 100 degrees C for 5 min. The heat-resistant epitopes are found by immunoblotting on multiple polypeptides after solubilization of the rickettsiae at temperatures of 56 degrees C or higher. Most, but not all, antibodies to the heat-sensitive epitopes passively protected mice against two 50% lethal doses of R. rickettsii, whereas none of the antibodies to heat-resistant epitopes did.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D006358:Hot Temperature; D007116:Immunization, Passive; D051379:Mice; D009500:Neutralization Tests; D011489:Protein Denaturation; D012284:Rickettsia rickettsii / Q000276:immunology*; D012373:Rocky Mountain Spotted Fever / Q000517:prevention & control
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D000939:Epitopes