Mediator release from basophilic cells derived from cultured cord blood cells.
Mononuclear cells from human umbilical cord blood were separately cultured either in the presence of phorbol ester-phytohemagglutin-leukocyte-conditioned medium (PE-PHA-LCM) or PHA-LCM. Cells cultured in PE-PHA-LCM released histamine following calcium ionophore and compound 48/80 challenge. However, there was a similar histamine release from cells cultured in the presence of PHA-LCM following calcium ionophore but not following compound 48/80 challenge. Neutrophil chemotactic activity (NCA), recognized to be one of the markers for mast cell activation, was detected following calcium ionophore and anti-IgE challenge from the supernatant of cells cultured in the presence of PHA-LCM, suggesting unique features in the cells studied.
D001491:Basophils / Q000187:drug effects / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D002630:Chemotactic Factors / Q000378:metabolism; D005089:Exocytosis / Q000187:drug effects*; D005312:Fetal Blood / Q000166:cytology*; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D007231:Infant, Newborn; D016209:Interleukin-8; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000494:pharmacology; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D002630:Chemotactic Factors; D016209:Interleukin-8; D008222:Lymphokines; D000001:Calcimycin; D003189:p-Methoxy-N-methylphenethylamine