Immunofluorescence demonstration of transdermal deposition of mucopolysaccharides into the skin.
The penetration into the skin of topically applied mucopolysaccharide polysulfate (MPS, Hirudoid) was demonstrated by an immunohistochemical technique. MPS-dependent fluorescence was detected in skin sections of nude mice and guinea pigs obtained by cryostat and synthetic resin histology. Deposition of MPS in corium and subcutis was shown by the fluorescence of fine granules in the ground substance and of cellular elements resembling fibrocytes. The specificity of the antigen-antibody reaction has also been demonstrated: The reactivity of the human hyperimmune antisera was completely abolished by pre-incubation with MPS; normal serum or goat-anti-human-FITC-IgG alone gave no fluorescent reaction.
D000279:Administration, Cutaneous; D000818:Animals; D005260:Female; D005455:Fluorescent Antibody Technique; D006025:Glycosaminoglycans / Q000032:analysis / Q000493:pharmacokinetics*; D006168:Guinea Pigs; D007120:Immunochemistry; D008297:Male; D051379:Mice; D008819:Mice, Nude; D012869:Skin Absorption; D013194:Staining and Labeling
D006025:Glycosaminoglycans