Cloning of a developmentally regulated tegument antigen of Schistosoma mansoni.
We have cloned a gene encoding a 22.6 kDa antigen from a Schistosoma mansoni cDNA library. Northern blots indicate that transcription of this antigen occurs in adults and sporocysts but not in cercariae, eggs or in newly-transformed schistosomula. Immunoprecipitation and Western blotting with specific antisera indicate that the antigen is not detectable in the newly transformed schistosomulum but appears within 24 h of schistosomulum transformation. Indirect immunofluorescence of adult worms shows this protein to be located in the tegument.
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D007158:Immunologic Techniques; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012550:Schistosoma mansoni / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D014158:Transcription, Genetic
D000947:Antigens, Helminth; D011993:Recombinant Fusion Proteins; D012313:RNA; D004247:DNA