Decreased nucleic acid synthesis in low-dose-irradiated lymphocytes--effect of TCGF addition.
Flow cytometry was used to evaluate nucleic acid synthesis in irradiated mixed lymphocyte cultures (MLC) compared to nonirradiated control cultures. Two staining methods were used (propidium iodide and acridine orange). We showed that RNA and DNA synthesis are retarded in MLC receiving 0.2 Gy. This effect was reversed by lymphocyte growth factor.
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects / Q000528:radiation effects; D004247:DNA / Q000096:biosynthesis*; D005260:Female; D007376:Interleukin-2 / Q000494:pharmacology*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D012313:RNA / Q000096:biosynthesis*
D007376:Interleukin-2; D012313:RNA; D004247:DNA