title: Separation of methylated free bile acids from their taurine and methyl glycine conjugates by thin-layer chromatography.
abstract: Class separation of methylated free bile acids from bile acids conjugated with taurine and methylglycine was accomplished using a solvent system of 2,2,4-trimethylpentane-absolute ethanol 10:1 (v/v). By developing a silica thin-layer plate two times with solvent in a Brinkmann sandwich tank, the difficult resolution between methyl cholate and methyl glycolithocholate was achieved. Evidence is presented that this separation system may be useful as a preparative step in the analysis of bile acids by gas-liquid chromatography or high pressure liquid chromatography.--Bolt, M. J. G. Separation of methylated free bile acids from their taurine and methyl glycine conjugates by thin-layer chromatography.
mesh_terms: D001647:Bile Acids and Salts / Q000302:isolation & purification*; D002855:Chromatography, Thin Layer / Q000379:methods*; D008745:Methylation; D012521:Sarcosine / Q000302:isolation & purification; D013654:Taurine / Q000302:isolation & purification
chemical_list: D001647:Bile Acids and Salts; D013654:Taurine; D012521:Sarcosine