Trypanosoma cruzi: cell type dependent distribution of a tubulin domain in mammalian stages.
The distribution of tubulin domains in the mammalian stages of Trypanosoma cruzi was investigated by using monoclonal antibodies elicited against bovine brain tubulin. Western blotting performed on T. brucei trypomastigotes and T. cruzi epimastigotes showed that the monoclonal antibodies 16D3 and 24E3 reacted only with tubulin in these cell types. Indirect immunofluorescence revealed that, whereas 16D3 stained all microtubules, including subpellicular microtubules, the epitope defined by 24E3 was found in only a part of the tubulin pool of amastigotes and intermediate stages infecting murine fibroblasts and of broad trypomastigotes; the staining was limited to the basal bodies and the distal region of the flagellar adhesion zone in these developmental forms. By contrast, slender trypomastigotes did not exhibit any reaction with 24E3. These results are consistent with a transformation of broad trypomastigotes into slender trypomastigotes during which the tubulin domain recognized by 24E3 would undergo modifications leading to its complete masking in slender forms. The morphogenesis of the mammalian stages of T. cruzi would involve modifications of the tubulin molecule.
D000818:Animals; D000911:Antibodies, Monoclonal; D002454:Cell Differentiation; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D008870:Microtubules / Q000276:immunology / Q000502:physiology*; D009024:Morphogenesis; D014349:Trypanosoma cruzi / Q000648:ultrastructure*; D014404:Tubulin / Q000276:immunology / Q000502:physiology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D014404:Tubulin