Isolation of specific antigens from Angiostrongylus cantonensis. 2. By affinity chromatography.
The crude adult worm extract of Angiostrongylus cantonensis was subjected to a series of affinity chromatography for selective removal of host antigens as well as cross-reactive components with other helminths. The purified fraction designated as AC(p) with molecular weights ranging from 10,000-42,000 was found to contain antigenic components specific to A. cantonensis as determined by immunoelectrophoresis and enzyme-linked immunosorbent assay.
D000801:Angiostrongylus / Q000276:immunology*; D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D007122:Immunoelectrophoresis; D008681:Metastrongyloidea / Q000276:immunology*; D008970:Molecular Weight
D000947:Antigens, Helminth; D000939:Epitopes