Increased levels of serum lipofuscin derived from 3-hydroxyanthranilic acid in patients with chronic renal failure.
Normal Caucasian male sera incubated with 3-hydroxyanthranilic acid to generate soluble lipofuscin were studied together with unincubated serum samples from uremic Caucasian males, using the methods of Schwertner & Hawthorne in order to identify a fluorescent substance found by them to increase in uremic sera. Ethanol extracts of uremic sera, of normal sera containing this soluble lipofuscin and of same normal serum blanks were prepared. Reversed-phase thin-layer chromatograms of the extracts developed with methanol-water (40:60, v./v.), displayed one significant spot per sample, with RF values of 0.89 +/- 0.02. The spots showed blue fluorescence in 366 nm ultraviolet light. Aqueous solutions of the spots from uremic sera and from 3-hydroxyanthranilic acid-incubated normal sera produced closely similar fluorescence excitation shoulders and maxima at approximately 321 nm and emission maxima at 402 +/- 3 nm with significantly higher intensities than the normal. Thin-layer chromatograms of the ethanol extracts were also prepared on silica gel G developed with ethanol. The uremic, the 3-hydroxyanthranilic acid-incubated normal sera and the normal blank sera showed identical patterns in 366 nm light. The findings demonstrate that serum lipofuscin derived from 3-hydroxyanthranilic acid either in vivo or in vitro yields the fluorescent substance or component separated by ethanol extraction and reversed-phase thin-layer chromatography and that this serum lipofuscin present at low concentration in normal sera increases in uremic sera.
D015095:3-Hydroxyanthranilic Acid / Q000097:blood / Q000378:metabolism*; D000328:Adult; D002855:Chromatography, Thin Layer; D005453:Fluorescence; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood*; D008062:Lipofuscin / Q000097:blood*; D008297:Male; D010860:Pigments, Biological / Q000097:blood*; D062367:ortho-Aminobenzoates / Q000378:metabolism*
D008062:Lipofuscin; D010860:Pigments, Biological; D062367:ortho-Aminobenzoates; D015095:3-Hydroxyanthranilic Acid