Effect of nifedipine on 67k calcimedin in cultured macrophages and smooth muscle cells.
The calcium signal in regulation is mediated by specific calcium-binding proteins. The calcimedins, a newly described set of proteins, bind hydrophobic domains in a calcium-dependent manner and may play a role in calcium signal mediation. We previously showed that mouse peritoneal macrophages contain the 67k calcimedin and that thioglycolate elicited inflammatory macrophages appeared to have elevated levels of the 67k calcimedin. We now report that the cultured macrophage-like cell line J774A.1, as well as the cultured smooth muscle cell line DDT1MF-2, contain the 67k calcimedin as determined by indirect immunofluorescence. We further report that the level of fluorescence is increased in these cell lines after treatment with the calcium channel blocker nifedipine.
D000818:Animals; D017302:Annexins; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005455:Fluorescent Antibody Technique; D008264:Macrophages / Q000187:drug effects*; D008970:Molecular Weight; D009130:Muscle, Smooth / Q000187:drug effects*; D009543:Nifedipine / Q000494:pharmacology*; D013194:Staining and Labeling
D017302:Annexins; D002135:Calcium-Binding Proteins; D009543:Nifedipine