Sequence homology analysis of proteins by chemical cleavages: using a mono and two dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
The examination of possible sequence homology in proteins using SDS-PAGE systems after chemical cleavage is described. After SDS-PAGE, the establishment of amino acid compositions, the techniques of staining gel and five different methods of chemical cleavages (cyanogen bromide, BNPS-skatole, hydroxylamine, formic acid and nitrothiocyano benzoic acid) have been used for peptide mapping studies. Potential applications of this technique are discussed from both the biochemical and immunochemical point of view.
D000595:Amino Acid Sequence; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D011506:Proteins; D012689:Sequence Homology, Nucleic Acid; D012967:Sodium Dodecyl Sulfate; D013194:Staining and Labeling
D011506:Proteins; D012967:Sodium Dodecyl Sulfate