title: Molecular cloning and nucleotide sequence of the cDNA for sperm-specific lactate dehydrogenase-C from mouse.
abstract: Mouse sperm-specific lactate dehydrogenase-C (LDH-C) cDNA was cloned and sequenced from lambda gt11 expression library. The LDH-C cDNA insert of 1236 bp consists of the protein-coding sequence (999 bp), the 5' (54 bp) and 3' (113 bp) non-coding regions, and the poly(A) tail (70 bp). The Northern blot analysis of poly(A)-containing RNAs from mouse testes and liver indicates that the LDH-C gene is expressed in testes but not in liver, and that its mRNA is approx. 1400 nucleotides in length. The nucleotide and amino acid sequences of the mouse LDH-C cDNA show 73% and 72% homologies, respectively, with those of the mouse LDH-A. The Southern blot analysis of genomic DNAs from mouse liver and human placenta indicates the presence of multiple LDH-C gene-related sequences.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D008099:Liver / Q000032:analysis; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D010920:Placenta / Q000032:analysis; D011061:Poly A / Q000032:analysis; D012313:RNA / Q000032:analysis; D013094:Spermatozoa / Q000201:enzymology*; D013737:Testis / Q000032:analysis
chemical_list: D007527:Isoenzymes; D011061:Poly A; D012313:RNA; D004247:DNA; D007770:L-Lactate Dehydrogenase