title: Erythrocytes and age. Disintegration of erythrocytes by brilliant cresyl blue in correlation to age.
abstract: The author describes changes in the disintegration of erythrocytes by brilliant cresyl blue in correlation to age, in rats aged 21, 42, 90-105, 340-360 and 690-720 days. The erythrocytes were incubated for 4 hours in an isotonic NaCl solution, in Krebs-Ringer solution and in each of these solutions plus brilliant cresyl blue. Disintegration in plain NaCl solution was found to be the greatest in the case of erythrocytes from 690- to 720-day-old rats. In the same solution plus brilliant cresyl blue, the rate of disintegration was very high in 21-day-old, 42-day-old and 690- to 720-day-old animals; at 90-105 days it was lower and at 340-360 days it was the lowest. Disintegration of erythrocytes in plain Krebs-Ringer solution was the lowest at 21 and 42 days; in the other age groups it was slightly higher. On adding brilliant cresyl blue, the rate of disintegration rose significantly in 21-, 42- and 690- to 720-day-old animals; at 90-105 days and 340-360 days it was no different from disintegration in plain Krebs-Ringer solution. It can be seen from the results that the rate of brilliant cresyl blue-induced erythrocyte disintegration is dependent on the age of the animals from which the erythrocytes are taken.
mesh_terms: D000375:Aging; D000818:Animals; D004912:Erythrocytes / Q000187:drug effects / Q000502:physiology*; D006461:Hemolysis / Q000187:drug effects; D007700:Kinetics; D010078:Oxazines / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling; D013997:Time Factors
chemical_list: D010078:Oxazines; C006797:Brilliant Cresyl Blue