Corneal storage in MK medium and K-Sol. Effect on ionic and non-ionic fluxes.
Rabbit corneas were stored at 4 degrees C for 3, 7 or 14 days in either modified MK medium or K-Sol. Corneal endothelial permeability to inulin following storage in modified MK was significantly less at each time examined than that found in corneas stored for either 3, 7 or 14 days in K-Sol. Inulin permeability after storage in K-Sol was increased at all times relative to unstored control corneal tissue, but only at 7 and 14 days in MK medium. Dextran permeability was similar following 3 days of storage in either solution, but dextran permeability following storage in modified MK was significantly less than the values found in corneas stored for 7 and 14 days in K-Sol. Dextran permeability was not significantly increased relative to control, at any storage time in MK medium but was increased at 7 and 14 days in K-Sol. Inulin and dextran permeabilities after storage in MK medium were maintained more closely to values found in fresh tissue than corneas stored in K-Sol. Net endothelial sodium fluxes following storage in modified MK medium were markedly less than those found in corneas stored for 3, 7 and 14 days in K-Sol. Net sodium fluxes are maintained better in K-Sol than in MK medium relative to control values. Net bicarbonate fluxes following storage in modified MK medium were significantly less than the 3-day values in K-Sol, but similar to the values after 7 and 14 days of K-Sol storage.(ABSTRACT TRUNCATED AT 250 WORDS)
D000818:Animals; D001639:Bicarbonates / Q000493:pharmacokinetics; D002807:Chondroitin / Q000031:analogs & derivatives; D002809:Chondroitin Sulfates / Q000494:pharmacology; D003315:Cornea / Q000502:physiology*; D003470:Culture Media / Q000494:pharmacology; D003911:Dextrans / Q000493:pharmacokinetics; D004728:Endothelium, Corneal / Q000187:drug effects; D006531:HEPES / Q000494:pharmacology; D007444:Inulin / Q000493:pharmacokinetics; D009926:Organ Preservation; D010879:Piperazines / Q000494:pharmacology; D011817:Rabbits; D012964:Sodium / Q000493:pharmacokinetics
D001639:Bicarbonates; D003470:Culture Media; D003911:Dextrans; C051174:K-Sol preserving medium; D010879:Piperazines; D007444:Inulin; D002807:Chondroitin; D002809:Chondroitin Sulfates; D012964:Sodium; D006531:HEPES