Humoral autoimmune response to rat male accessory glands. Immunogenicity of different autoantigen moiety for male and female rats.
The efficiency of autoantigen fragments (Fa and Fc) to trigger a memory autoimmune response in rats primed with chemically modified rat male accessory glands (MRAG) was assayed by reimmunizing them with the fragments coupled to human serum albumin (Fa-HSA and Fc-HSA). The humoral immune response assay by ELISA and passive hemagglutination revealed that a high level of antibodies reactive with the autoantigen of accessory glands is triggered by Fa-HSA in male and by Fc-HSA in female rats, whereas Fc in male and Fa in female rats did not stimulate appreciable levels of antibodies. Furthermore, the specificities of male and female antibodies were directed mainly to Fa and Fc epitopes, respectively. Therefore, Fa behaved like a more immunogenic fragment for male rats and Fc for female rats.
D000818:Animals; D000918:Antibody Specificity; D001323:Autoantibodies / Q000096:biosynthesis* / Q000276:immunology; D001324:Autoantigens / Q000276:immunology*; D055598:Chemical Phenomena; D002621:Chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005260:Female; D005837:Genitalia, Male / Q000276:immunology*; D006386:Hemagglutination Tests; D007106:Immune Sera / Q000276:immunology; D008297:Male; D010446:Peptide Fragments / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D012737:Sex Factors
D001323:Autoantibodies; D001324:Autoantigens; D000939:Epitopes; D007106:Immune Sera; D010446:Peptide Fragments