25-Hydroxycholesterol promotes myelin basic protein-induced leakage of phospholipid vesicles.
Among several cholesterol oxidation products, 25-hydroxycholesterol is particularly potent in enhancing basic protein-induced carboxyfluorescence leakage from liposomes. Both myelin basic protein and poly(L-arginine) are effective at submicromolar concentrations in stimulating this vesicle rupture. Leakage is greatly augmented by the presence of only 1% of the 25-hydroxycholesterol. The possible relevance of these findings to myelin pathology is discussed.
D002784:Cholesterol; D005452:Fluoresceins; D006801:Humans; D006888:Hydroxycholesterols / Q000494:pharmacology*; D008081:Liposomes / Q000378:metabolism*; D004676:Myelin Basic Protein / Q000494:pharmacology*; D010455:Peptides; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D013050:Spectrometry, Fluorescence
D005452:Fluoresceins; D006888:Hydroxycholesterols; D008081:Liposomes; D004676:Myelin Basic Protein; D010455:Peptides; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; C015462:polyarginine; C024098:6-carboxyfluorescein; C020888:1,2-dielaidoylphosphatidylethanolamine; C007997:25-hydroxycholesterol; C045132:4-carboxyfluorescein; D002784:Cholesterol