Substance P action on inositol phospholipids in rat adrenal medulla slices.
The effect of SP (1.10(-6) M) on the 32P incorporation into inositol phospholipids from rat adrenal medulla slices has been examined. After 20 s, a decreased 32P incorporation into all inositol phospholipids was found (most expressed for phosphatidyl-inositol-4,5-bisphosphate) without changes in phosphatidylcholine/phospatidylserine fraction. During 5 min exposure to SP the 32P incorporation into phosphatidylinositol-4,5-bisphosphate returned up to the control level, whereas that into phosphatidylcholine/phosphatidylserine fraction strongly decreased. A within-one-sample comparison of the radioactivity of each phospholipid against that in the phosphatidylcholine/phosphatidylserine fraction reveals a "phosphatidylinositol effect". A possible regulation mechanism of catecholamine secretion by SP in relation to changes in inositol phospholipids is discussed.
D000313:Adrenal Medulla / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D066298:In Vitro Techniques; D008297:Male; D010716:Phosphatidylinositols / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013373:Substance P / Q000494:pharmacology*
D010716:Phosphatidylinositols; D010743:Phospholipids; D013373:Substance P; D010727:Phosphoric Diester Hydrolases