Dimethyl sulfoxide-induced augmentation of adenosine-adenylate cyclase response of pig skin epidermis.
Adenosine-adenylate cyclase response in pig skin epidermis showed a specific increase after long-term (24 h) incubation in the presence of 0.5%-1% dimethyl sulfoxide (DMSO). There was no significant difference between control and DMSO-treated epidermis with regard to cyclic AMP (cAMP) phosphodiesterase activity. DMSO had no effect on the basal cAMP levels of epidermis; beta-adrenergic and histamine-adenylate cyclase responses were not affected. The direct addition of DMSO at the time of incubation with various adenylate cyclase stimulators (adenosine, epinephrine, and histamine) had no effect on agonist-induced cAMP accumulation effects. It was concluded that DMSO affected epidermal keratinocytes during long-term incubation, resulting in a specific increase in the adenosine-adenylate cyclase response. Although the biological significance of this DMSO effect remains to be determined, it should be kept in mind when using DMSO as a solvent for various chemicals in the experiments dealing with epidermal keratinocytes in vitro.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism; D007633:Keratins / Q000378:metabolism; D013268:Stimulation, Chemical; D013552:Swine
D007633:Keratins; D000242:Cyclic AMP; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D000262:Adenylyl Cyclases; D004121:Dimethyl Sulfoxide