[Isolation of monospecific Salmonella anti-O-4 antibodies by affinity chromatography and their use in immunoenzyme reactions].
An immunosorbent with Salmonella typhimurium 1021 O-antigen has been obtained on the basis of polymer-modified porous glass. Binding has been effected due to the presence of NH2-groups on the carrier and aldehyde groupings on O-antigen previously oxidized with sodium periodate. Specific anti-O-4 antibodies have been isolated with the use of this immunosorbent, and on the basis of these antibodies peroxidase conjugates have been obtained. This preparation has made it possible to establish the parameters of EIA for the detection of Salmonella O-4 antigens in the assay involving the use of the sandwich technique. The method has proved to be sufficiently simple, sensitive and specific. It is recommended for the detection of Salmonella O-4 antigen in biological fluids.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology*; D002846:Chromatography, Affinity / Q000379:methods; D007124:Immunoenzyme Techniques; D007163:Immunosorbent Techniques; D019081:O Antigens; D011817:Rabbits; D012486:Salmonella typhimurium / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D019081:O Antigens
Poluchenie monospetsificheskikh sal'monelleznykh anti-O-4 antitel metodom affinnoĭ khromatografii i ikh ispol'zovanie v immunofermentnykh reaktsiiakh.