[Characteristics of the ribonucleoprotein of the measles virus persisting in a human cell culture].
The properties of virus-specific RNPs recovered from human HEp-2 and L-41 cells chronically infected with measles virus were studied in comparison with those of RNPs formed in acute infection of L-41 cells. The persisting RNP was shown to contain nucleoprotein not differing in the electrophoretic mobility from the same protein of measles virus virions, and RNA in the persisting RNP was found to be insensitive to the action of RN-ase. RNP from chronically infected cells had a changed ultrastructure and conformation as compared with RNP of the original virus and, unlike the latter had no infectivity upon transfection of the sensitive cells by calcium-phosphate precipitation. No differences in relationships of RNP with the cytoskeleton of the infected cells in the acute and chronic infection were observed.
D000956:Antigens, Viral / Q000032:analysis; D002213:Capsid / Q000032:analysis / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006801:Humans; D008457:Measles / Q000382:microbiology; D008459:Measles virus / Q000032:analysis / Q000235:genetics / Q000472:pathogenicity*; D008854:Microscopy, Electron; D012261:Ribonucleoproteins / Q000032:analysis* / Q000302:isolation & purification; D014162:Transfection; D014758:Viral Core Proteins / Q000032:analysis / Q000302:isolation & purification; D014764:Viral Proteins / Q000032:analysis* / Q000302:isolation & purification; D014776:Virus Cultivation
D000956:Antigens, Viral; D000939:Epitopes; D012261:Ribonucleoproteins; D014758:Viral Core Proteins; D014764:Viral Proteins
Kharakteristika ribonukleoproteida virusa kori, persistiruiushchego v kul'ture kletok cheloveka.