The isolation of high-molecular-weight DNA from plants.
A procedure to isolate high-molecular-weight DNA from plant materials has been devised. With this procedure, high-molecular-weight DNA suitable for Southern transfer experiments has been isolated from over 30 plant species including angiosperms (both dicots and monocots), a gymnosperm, members of other divisions, and two microorganisms.
D002021:Buffers; D002848:Chromatography, DEAE-Cellulose; D004247:DNA / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D010944:Plants / Q000032:analysis*; D012313:RNA / Q000302:isolation & purification; D013045:Species Specificity
D002021:Buffers; D012313:RNA; D004247:DNA