Analysis of antibody microprobe autoradiographs by computerized image processing.
A computerized digitizing method to analyze the autoradiographs derived from the antibody microprobe technique is described. The method enables the averaging of groups of microprobes that have been placed in similar locations in the central nervous system during the same physiological stimulation. Control and experimental groups of microprobes can be compared, and a level of significance for the release of a neuropeptide obtained. In addition estimates can be made of the concentration of peptide present in regions of release.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D001345:Autoradiography / Q000379:methods*; D004594:Electrophysiology / Q000295:instrumentation* / Q000379:methods; D007091:Image Processing, Computer-Assisted; D008839:Microelectrodes; D009479:Neuropeptides / Q000276:immunology* / Q000378:metabolism; D013116:Spinal Cord / Q000378:metabolism; D013373:Substance P / Q000276:immunology / Q000378:metabolism
D000906:Antibodies; D009479:Neuropeptides; D013373:Substance P