[Possibilities to elevate the factor VIII yield].
By referring initially to remarks about the structure and function of the coagulation factor VIII and about the manufacture and demand for preparations for the substitution treatment in patients affected with haemophilia A, possibilities are presented how to increase the collection of factor VIII by applying intensive measures. These involve the impact on the basic material (including donors) as well as process variables within the range of plasma collection and process technique. On the basis of own research results and data from literature the following measures can be introduced and evaluated as far as their effect on the collection of factor VIII is concerned: Donor testing, selection: increased by 25% approximately Plasmapheresis, blood bags: (prerequisite for certain technological measures) Thawing technique: increase by 20-30% approximately (thaw siphon) Citrate-free anticoagulant: increase by 30% approximately (e.g. heparin) Donor conditioning: increased by 200-400% approximately (DDAVP) The establishment of possible and reasonable combinations of measures can contribute to intensify the collection of factor VIII. The advantages to be expected are mentioned. The level of gene-technological collection of factor VIII is dealt with prospectively.
D001782:Blood Donors; D001793:Blood Preservation / Q000379:methods; D002999:Clone Cells; D004356:Drug Storage; D005169:Factor VIII / Q000302:isolation & purification* / Q000627:therapeutic use; D005615:Freezing; D006467:Hemophilia A / Q000188:drug therapy; D006801:Humans; D008722:Methods; D010956:Plasmapheresis; D014842:von Willebrand Diseases / Q000188:drug therapy
D005169:Factor VIII
Möglichkeiten zur Steigerung der Faktor-VIII-Gewinnung.