title: Chromosome heteromorphisms in the gorilla karyotype. Analyses with distamycin A/DAPI, quinacrine and 5-azacytidine.
abstract: The chromosomes of one male and three female gorillas were extensively studied with various regional banding methods. The chromosomes were stained with the fluorescent dyes quinacrine mustard and distamycin A/DAPI (DA/DAPI), which label different subsets of heterochromatin in the chromosome complement. Furthermore, lymphocyte cultures were treated with the cytidine analog 5-azacytidine (5-azaC). The 5-azaC-induced undercondensations were found in most of the DA/DAPI-bands as well as in many telomeric C-bands. The karyotype of the gorilla exhibits a considerable number of heterochromatin variants. Of the different types of heteromorphisms noted, the most striking is that involving the short arm regions of chromosomes 12 to 16 and 23 (satellite stalk regions) and the paracentromeric heterochromatin of chromosomes 17 and 18. There also are numerous heteromorphic C-bands localized in the telomeric regions of homologous chromosome arms. In comparison, only few heteromorphisms occur between C-bands in the centromeric and pericentromeric regions of homologs. Finally, a variability in the fluorescence intensity of quinacrine-bright satellites in the short arms of chromosomes 12 to 16, 22, and 23 is observed.
mesh_terms: D000818:Animals; D001374:Azacitidine / Q000494:pharmacology; D002478:Cells, Cultured; D002871:Chromosome Banding; D002875:Chromosomes / Q000187:drug effects / Q000648:ultrastructure*; D005260:Female; D006071:Gorilla gorilla / Q000235:genetics*; D006570:Heterochromatin / Q000648:ultrastructure*; D007621:Karyotyping; D008214:Lymphocytes / Q000166:cytology; D008297:Male; D013194:Staining and Labeling
chemical_list: D006570:Heterochromatin; D001374:Azacitidine