Improved separation of fetal hemoglobin by mini-column chromatography.
We present chromatographic conditions for separating and measuring fetal hemoglobin on a 5-cm column of Sephadex CM-50. A distinct fetal band is evident, even when present at less than 1% of total hemoglobin. Precision (CV) of better than 5% is achievable.
D002845:Chromatography; D003920:Diabetes Mellitus / Q000097:blood; D005319:Fetal Hemoglobin / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011489:Protein Denaturation; D013223:Statistics as Topic
D005319:Fetal Hemoglobin