[Dependence of the redistribution of ligand-receptor complexes in cultured cells on the type of growth and on the cell cycle phase].
The process of active redistribution of ligand-receptor complexes on the surface of both suspension (LS) and adapted for growing in monolayer (LMS) sublines of mouse fibroblasts L was studied. The binding of ligands to its specific receptors on the surface of the LS cells induced an accumulation of ligand-receptor complexes on one pole of a cell with the formation of a typical cap. Under the same conditions in the LSM cells the cleaning of processes and lamello-plasma surfaces from the ligand-receptor complexes was registered. The binding of ligands to the surface of the LSM cells, detached with EDTA, induced the same capping process, as in the case of the LS cells. No differences were found in the redistribution capacity of the ligand-receptor complexes in synchronized cultures of the LS and LSM cells in the G1 and S phase of the cycle. But such a redistribution was not registered on the surface of cells in metaphase and anaphase. The accumulation of ligand-receptor complexes was found in the region of cleavage between daughter cells in telophase. These results are in a good agreement with the well-known data on the changes in the cytoskeleton organization during transition from a monolayer to a suspension state and during mitosis.
D000818:Animals; D002453:Cell Cycle; D007399:Interphase; D007739:L Cells / Q000648:ultrastructure*; D008024:Ligands; D051379:Mice; D011940:Receptor Aggregation; D013194:Staining and Labeling / Q000379:methods; D013499:Surface Properties
D008024:Ligands
Zavisimost' pereraspredeleniia ligand-retseptornykh kompleksov u kul'tiviruemykh kletok ot tipa rosta i fazy kletochnogo tsikla.