[A new method of DNA and RNA transfer onto nitrocellulose filters during blot hybridization].
A quick (1-2 hour) method of DNA and RNA transfer onto nitrocellulose filters for subsequent blot-hybridization was elaborated. The main features of the method proposed are, firstly, almost complete exclusion of the mechanical impact on the gel and, secondly, addition to the transfer medium (20 X SSC) of a chaotropic agent, 0.5 M NaClO4. The latter results in a slight dissolution of the gel matrix and, on the other hand, somewhat increases the binding of the nucleic acid to the nitrocellulose. The method shortens significantly the time of DNA or RNA transfer at equal, or even higher, quality of hybridization.
D000818:Animals; D010582:Bacteriophage lambda / Q000032:analysis; D003101:Collodion; D004247:DNA / Q000032:analysis*; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D004587:Electrophoresis, Agar Gel; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000032:analysis*; D051381:Rats
D012313:RNA; D003101:Collodion; D004247:DNA; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI
Novyĭ sposob perenosa DNK i RNK na nitrotselliuloznye fil'try v metode blot-gibridizatsii.