Studies on suppressor factors produced by T-cell hybridomas. I. Characterization of antigen-specific suppressor factors.
Antigen-specific and antigen-nonspecific suppressor T cells were generated when spleen cells prepared from C57BL/6J (H-2b) were incubated with trinitrophenylated polyacrylamide beads (TNP-PAA) in vitro. T hybridomas were prepared by fusion of spleen cells cultured with TNP-PAA for 4 days and the thymoma cell line BW5147. More than 100 hybridomas were generated, and 15 of them suppressed the anti-TNP PFC response of fresh spleen cells cultured with TNP-PAA. The suppression was antigen specific with three of these five hybridoma supernatants tested. Hybridomas that caused antigen-specific suppression secrete factors which bring about suppression of the anti-TNP PFC response by spleen cells cultured with TNP-PAA. These hybridoma supernatants which cause antigen-specific suppression typically depressed the anti-TNP PFC response by 60% while depressing anti-SRBC PFC response by only 10%. The antigen-specific suppressor factors were bound to a TNP-BGG column but not to a BGG column. The suppressor factors, purified by affinity chromatography on a TNP-BGG column, were bound to anti-I-Jb antibody.
D000180:Acrylic Resins; D000818:Animals; D002474:Cell-Free System; D002478:Cells, Cultured; D000939:Epitopes / Q000276:immunology*; D006241:Haptens / Q000276:immunology; D006825:Hybridomas / Q000276:immunology / Q000378:metabolism*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013491:Suppressor Factors, Immunologic / Q000032:analysis / Q000096:biosynthesis*; D050378:T-Lymphocytes, Regulatory / Q000378:metabolism*; D014301:Trinitrobenzenes / Q000276:immunology; D005719:gamma-Globulins / Q000276:immunology; D000099166:Polyacrylamides
D000180:Acrylic Resins; D000939:Epitopes; D006241:Haptens; D013491:Suppressor Factors, Immunologic; D014301:Trinitrobenzenes; D005719:gamma-Globulins; D000099166:Polyacrylamides