Allele-specific methylation of the human c-Ha-ras-1 gene.
The methylation status of individual c-Ha-ras-1 alleles in human cells was measured by Hpall/Mspl analysis of a polymorphic (VTR) region. The gene was extensively methylated in leukocytes and sperm, with the former exhibiting a highly specific methylation pattern. Individual ras alleles were differentially methylated at the VTR region in fetal fibroblasts and immortal cell lines. A new polymorphism in the 5'-flanking region of the gene was also detected. The presence or absence of an Xhol site showed a striking and complete concordance with the length of the VTR region and suggested that the site had been lost by a previous allele-specific mutation.
D000483:Alleles; D002460:Cell Line; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D019003:Deoxyribonuclease HpaII; D004848:Epithelium / Q000378:metabolism; D005333:Fetus; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D008745:Methylation; D009857:Oncogenes; D012516:Osteosarcoma / Q000378:metabolism; D011110:Polymorphism, Genetic; D013094:Spermatozoa / Q000378:metabolism
D004247:DNA; D004262:DNA Restriction Enzymes; D019003:Deoxyribonuclease HpaII