Induction of fibronectin expression, actin cable formation, and entry into S phase following reexpression of T antigen in mouse macrophages transformed by the tsA640 mutant of SV40.
Mouse macrophages transformed by a temperature-sensitive mutant (tsA640) of simian virus 40 (SV40) were examined by immunofluorescence microscopy for fibronectin expression and actin distribution. Resting cultures of tsA640 transformants incubated at a temperature nonpermissive for SV40 large T antigen (39.0 degrees C) exhibited phagocytic activity and did not exhibit cellular fibronectin and actin cables, like primary cultures of resident macrophages. When the resting cultures were sparsely seeded and shifted down to the permissive temperature of 33.0 degrees C, expression of large T antigen in the nucleus, expression of fibronectin in the cytoplasm, and cellular entry into S phase occurred in that temporal order, followed by actin cable formation, cellular proliferation, and diminishment of phagocytic activity. The expression of T antigen and fibronectin was sensitive to actinomycin D and cycloheximide. The expression of fibronectin was insensitive to inhibitors of DNA synthesis, whereas the expression of actin cables was sensitive. These results suggest that SV40 T antigen leads macrophages to express fibronectin and actin cables, as well as resumption of cell proliferation, and that entry into S phase is not required for fibronectin expression but may be required for actin cable formation.
D000199:Actins / Q000378:metabolism*; D000818:Animals; D000957:Antigens, Viral, Tumor; D002460:Cell Line; D002472:Cell Transformation, Viral; D003599:Cytoskeleton / Q000648:ultrastructure; D004247:DNA / Q000096:biosynthesis; D005353:Fibronectins / Q000096:biosynthesis*; D007399:Interphase; D008264:Macrophages / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D009154:Mutation; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D013539:Simian virus 40 / Q000235:genetics / Q000276:immunology / Q000502:physiology; D013696:Temperature
D000199:Actins; D000957:Antigens, Viral, Tumor; D005353:Fibronectins; D012313:RNA; D004247:DNA