HIV-1 expression by T8 lymphocytes after transfection.
Acquired immune deficiency syndrome (AIDS) is an immunosuppressive disease associated with the depletion of T4 lymphocytes. Recently, an HIV-1 genome was molecularly cloned and shown to be fully infectious in vitro by transfection experiments. In this study, we show that HIV-1 can be transfected into T4 and T8 lymphocyte subpopulations and viral proteins and infectious virus particles are observed in both short- and long-term cultures. In addition, transfected T8 cells can be maintained in culture for long periods without apparent cytopathic effect.
D000956:Antigens, Viral / Q000235:genetics; D002478:Cells, Cultured; D005786:Gene Expression Regulation; D005814:Genes, Viral; D006678:HIV / Q000201:enzymology / Q000235:genetics*; D015488:HIV Antigens; D006801:Humans; D010957:Plasmids; D012194:RNA-Directed DNA Polymerase / Q000235:genetics / Q000378:metabolism; D013601:T-Lymphocytes / Q000382:microbiology*; D014162:Transfection
D000956:Antigens, Viral; D015488:HIV Antigens; D012194:RNA-Directed DNA Polymerase