Characterization of an idiotype-binding helper cell required for the formation of timothy grass pollen antigen-B-specific IgE.
Experiments were conducted using an excess of biosynthetically labeled timothy grass pollen antigen-B-specific (AgB-specific) T helper factor (THF) with enriched T cells, enriched B cells, or normal spleen cells to determine the site of the THF action. These studies indicated that the labeled THF bound preferentially to T cells, and analysis of the cell surface characteristics suggested that these T cells were Lyt 123+ cells. A panning technique using partially purified AgB-specific THF-coated Petri dishes depleted a population of idiotype-binding T cells that appears to be required for the formation of AgB-specific IgE antibody.
D000485:Allergens / Q000276:immunology; D000818:Animals; D001666:Binding Sites, Antibody; D000939:Epitopes; D007130:Immunoglobulin Idiotypes / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D000485:Allergens; D000939:Epitopes; D007130:Immunoglobulin Idiotypes