Immunohistochemical demonstration of hyalinosis-associated 90 kD glycoprotein in amyloid deposits of lichen amyloidosus.
Immunohistochemical methods were used to study the nature of the amyloid deposits in lichen amyloidosus, in nodular amyloidosis, and in the cutaneous amyloid deposits found in Finnish-type systemic amyloidosis. In every case the anti-keratin serum stained the epidermis and sweat ducts but not the amyloid itself. None of the amyloids stained with anti-sera to prealbumin, to serum amyloid A protein, or to the free kappa or lambda light chains of immunoglobulins. In lichen amyloidosus but not in the other types of amyloidosis the amyloid substance stained intensely with the anti-serum to 90 kD glycoprotein. This glyco-protein, which is present in the basal cells of the hair follicles of normal skin, was first isolated from the extensive cutaneous deposits of a patient with a nonamyloid disease. The demonstration of this glycoprotein in lichen amyloidosus but not in nodular amyloidosis suggest a difference in pathogenesis between the two diseases. Tests for 90 kD glycoprotein may prove to be of value in the differential diagnosis of cutaneous amyloidosis.
D000328:Adult; D000368:Aged; D000686:Amyloidosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D003937:Diagnosis, Differential; D005260:Female; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D006818:Hyalin / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D012871:Skin Diseases / Q000378:metabolism* / Q000473:pathology; D013194:Staining and Labeling
D006023:Glycoproteins