Silver staining of proteins: standardized procedure for two-dimensional gels bound to polyester sheets.
A system for the simultaneous silver staining of numerous two-dimensional gels bound to polyester sheets is proposed. Under controlled conditions, a very good reproducibility of staining can be obtained, even between different experiments. Procedures to avoid artifacts due to polyester sheets (background, silver mirror, and vertical streaking) are described.
D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D010940:Plant Proteins / Q000032:analysis; D011091:Polyesters; D011506:Proteins / Q000032:analysis*; D012834:Silver; D013194:Staining and Labeling / Q000295:instrumentation; D014908:Triticum / Q000032:analysis
D010940:Plant Proteins; D011091:Polyesters; D011506:Proteins; D012834:Silver