Production of T suppressor factor specific for the hapten picryl chloride requires both T suppressor cells and an antigen-specific, genetically restricted second signal.
We investigated the requirement for activation of T suppressor cells specific for the hapten picryl chloride and the release of hapten-specific T suppressor factor. Using an in vivo experimental system, we report that activation of T suppressor cells and the consequent release of T suppressor factor required two signals: one was provided by primed T suppressor cells, i.e. spleen cells from mice injected with the tolerogen picrylsulphonic acid, and the other was provided by the specific antigen in the context of H-2 gene products. Mechanisms by which the interaction between these two signals led to activation of T suppressor cells and the production of T suppressor factor, as well as a comparison with other antigen-specific suppressor systems, are discussed.
D000818:Animals; D002478:Cells, Cultured; D004220:Disulfides / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D005260:Female; D006183:H-2 Antigens / Q000276:immunology*; D006241:Haptens / Q000276:immunology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010084:Oxidation-Reduction; D010853:Picryl Chloride / Q000276:immunology*; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D013491:Suppressor Factors, Immunologic / Q000096:biosynthesis* / Q000235:genetics; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D004220:Disulfides; D000939:Epitopes; D006183:H-2 Antigens; D006241:Haptens; D013491:Suppressor Factors, Immunologic; D010853:Picryl Chloride