Inhibition of human pancreatic elastase II activity on human aortic elastin by human alpha 2-macroglobulin.
Human alpha 2-macroglobulin-human pancreatic elastase II binding were investigated using a homologous substrate, human aortic elastin, in order to test the enzymatic activity. We demonstrated that two moles of alpha 2-M are required to inhibit one mole of HPEII when the enzyme is added to a mixture of elastin and alpha 2-M. In addition, when the elastase-alpha 2-M complex is prepared under some circumstances, it exhibits an elastinolytic activity.
D001011:Aorta / Q000032:analysis*; D004549:Elastin / Q000378:metabolism*; D006801:Humans; D010179:Pancreas / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000511:alpha-Macroglobulins / Q000378:metabolism / Q000494:pharmacology*
D000511:alpha-Macroglobulins; D004549:Elastin; D010196:Pancreatic Elastase