A recognition site on synthetic helical oligonucleotides for monoclonal anti-native DNA autoantibody.
The binding site in native DNA for a murine monoclonal anti-DNA autoantibody was investigated by measurements of competitive binding of a series of synthetic helical oligonucleotides. The antibody bound to a (dG-dC)3 or (dG-dC)4 core in the center of a base-paired octadecanucleotide. Reactions of analogues containing modifications or substitutions at specific sites indicated that the antibody bound to portions of cytosine and guanine in the major groove, a limited region of the backbone, and the 2-amino group of one guanine in the minor groove. For these interactions to occur, the antibody combining site would straddle the backbone of one of the helical strands of DNA.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001323:Autoantibodies / Q000276:immunology*; D001483:Base Sequence; D004247:DNA / Q000276:immunology*; D000939:Epitopes; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D051379:Mice; D008958:Models, Molecular; D009690:Nucleic Acid Conformation
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D000939:Epitopes; D004247:DNA