Mixed precipitation in gel: a new method of identification and characterization of monoclonal antibodies.
A mixed precipitation in the gel (MPG) technique is suggested for detection and characterization of monoclonal antibodies (MAbs). The MPG is based on the formation of a mixed precipitate composed of an antigen, the corresponding MAb and precipitating polyclonal antiserum. MAb incorporated into the precipitate is revealed by Fab'-peroxidase conjugate added to polyclonal antiserum. The MPG technique was applied to hybridoma screening as well as for the antigen and epitope specificity analysis of different MAbs. The MPG is a one-step, simple, inexpensive technique and valuable for the study of any antigen which could be revealed by immunodiffusion.
D000362:Agar; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D002272:Carcinoembryonic Antigen / Q000276:immunology; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D005779:Immunodiffusion / Q000379:methods*; D051379:Mice; D051381:Rats; D000509:alpha-Fetoproteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D000939:Epitopes; D007106:Immune Sera; D000509:alpha-Fetoproteins; D000362:Agar