title: Chemical composition and immunological characterization of polysaccharide antigen from Eubacterium saburreum T18.
abstract: Two types of polysaccharide were obtained from the oral microorganism Eubacterium saburreum T18 by formamide extraction and subsequent gel filtration and ion-exchange chromatography. One polysaccharide, which was composed of D-glycero-D-galacto-heptose, had antigenic activity in an immunoprecipitation reaction with rabbit anti-T18 serum due to immunoglobulin M antibodies. The second polysaccharide was composed of D-glycero-D-manno-heptose and L-rhamnose, but it did not have immunoprecipitation activity. These polysaccharide antigens were not alkali labile and differed from E. saburreum L44 and T27 antigens, which were composed of D-glycero-D-galacto-heptose.
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis; D011232:Chemical Precipitation; D003773:Dental Plaque / Q000382:microbiology*; D000939:Epitopes; D005051:Eubacterium / Q000032:analysis*; D006539:Heptoses / Q000032:analysis / Q000276:immunology; D005779:Immunodiffusion; D011135:Polysaccharides, Bacterial / Q000032:analysis* / Q000276:immunology; D012210:Rhamnose / Q000032:analysis / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D000939:Epitopes; D006539:Heptoses; D011135:Polysaccharides, Bacterial; C026312:glycero-manno-heptose; C051913:glycero-galacto-heptose; D012210:Rhamnose