Thermostable ethanol-insoluble antigens of human heart muscle.
Organ-specific reactions were observed between thermostable ethanol-insoluble (BE) fraction of human heart muscle and its corresponding rabbit antiserum by means of tanned cell hemagglutination test, enzyme immunoassay, and gel precipitation test. Two heart-specific components could be identified using gel precipitation and immunoblotting procedures. Antibodies to human heart muscle BE combined also with heart BE preparations from mammals other than man; these interspecies cross-reactions included also rabbit heart BE pointing to the 'auto' nature of some of the antibodies under investigation. The heart antigens under study were distinct from the organ-specific antigen demonstrated by Espinosa and Kaplan in acid extract of human heart muscle.
D000372:Agglutination Tests; D000818:Animals; D000941:Antigens / Q000276:immunology / Q000302:isolation & purification*; D002417:Cattle; D003101:Collodion; D003429:Cross Reactions; D004355:Drug Stability; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000302:isolation & purification; D000431:Ethanol / Q000494:pharmacology*; D006358:Hot Temperature; D006801:Humans; D007124:Immunoenzyme Techniques; D009206:Myocardium / Q000276:immunology*; D009928:Organ Specificity; D011817:Rabbits; D012995:Solubility; D013552:Swine
D000941:Antigens; D000939:Epitopes; D000431:Ethanol; D003101:Collodion