The pR plasmid: a tool for discriminating between DNA lesions induced by different types of cytotoxic agents in cultured mammalian cells.
The LA-D cells, obtained by cotransformation of LTA mouse cells (tk- aprt-) with pR plasmid and with tk gene as selective marker, are significantly more resistant to UV light and 4-nitroquinoline-N-1-oxide than LTA control cells. In this work, we report that the LA-D cells exhibit different degrees of response to various DNA-damaging agents: wild-type survival to mitomycin, increased sensitivity to bleomycin, cis-diamminedichloroplatinum and N-methyl-N'-nitro-N-nitrosoguanidine. The pR plasmid could, therefore, play an important role in the DNA-repair mechanisms that modulate the cytotoxic effect of the DNA-inhibitory agents. The possible interactions between pR plasmid products and the different repair enzymes involved are discussed.
D000818:Animals; D001761:Bleomycin / Q000633:toxicity*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002945:Cisplatin / Q000633:toxicity*; D004249:DNA Damage; D008769:Methylnitronitrosoguanidine / Q000633:toxicity*; D016685:Mitomycin; D008937:Mitomycins / Q000633:toxicity*; D010957:Plasmids; D013937:Thymidine Kinase / Q000235:genetics; D014170:Transformation, Genetic; D014466:Ultraviolet Rays
D008937:Mitomycins; D001761:Bleomycin; D008769:Methylnitronitrosoguanidine; D016685:Mitomycin; D013937:Thymidine Kinase; D002945:Cisplatin