title: Differential effect of isoprinosine on lymphocyte proliferation: comparison of two tissue culture media.
abstract: Isoprinosine (ISP) has been reported to stimulate a variety of immunological responses. This study showed a differential effect of this drug on the proliferative response of human peripheral blood mononuclear cells to phytohemagglutinin (PHA). ISP was found to increase this proliferative response when Eagle's Minimum Essential Medium (MEM) was used. When Medium-199 (M-199) was used to culture the cells, no increase in the proliferative response was observed. This inhibition by M-199 of the ISP effect on PHA-stimulated cells has been traced to the presence of the purines adenine and hypoxanthine in M-199.
mesh_terms: D000225:Adenine / Q000494:pharmacology; D000328:Adult; D003470:Culture Media; D006801:Humans; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000494:pharmacology; D066298:In Vitro Techniques; D007288:Inosine / Q000031:analogs & derivatives*; D007542:Inosine Pranobex / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008875:Middle Aged; D010835:Phytohemagglutinins / Q000494:pharmacology
chemical_list: D003470:Culture Media; D007042:Hypoxanthines; D010835:Phytohemagglutinins; D019271:Hypoxanthine; D007288:Inosine; D000225:Adenine; D007542:Inosine Pranobex