A mechanism by which adenovirus virus-associated RNAI controls translation in a transient expression assay.
The mechanism by which adenovirus virus-associated RNAI stimulates translational efficiency in a transient-expression assay in 293 cells was investigated. We showed that DNA transfection leads to activation of a protein kinase that phosphorylates the alpha subunit of eucaryotic initiation factor 2 and, as a consequence, inhibition of polypeptide chain initiation. Cotransfection of a plasmid encoding adenovirus type 2 virus-associated RNAI recovered the translational capacity by preventing activation of the kinase.
D000260:Adenoviruses, Human / Q000235:genetics*; D002460:Cell Line; D014176:Protein Biosynthesis; D011494:Protein Kinases / Q000378:metabolism; D012313:RNA / Q000235:genetics*; D014162:Transfection; D019892:eIF-2 Kinase
D012313:RNA; D011494:Protein Kinases; D019892:eIF-2 Kinase