Activation of murine natural killer cells and macrophages by 8-bromoguanosine.
It has been shown that 8-bromoguanosine (8-BrGuo) activates T and B cells, but the underlying mechanism of this effect is not clear. We found that it also activates NK cells and macrophages by induction of IFN production. Culturing spleen cells with 8-BrGuo for 16 to 18 h induced cytotoxic activity to YAC cells. The cytotoxic cells expressed Qa-5, asialo-Gm-1, and NK-1.1 Ag. Similarly, preincubation of peptone-elicited peritoneal macrophages with 8-BrGuo induced macrophage cytolytic activity to P815 cells in an 18-h assay. Anti-IFN antibodies to IFN-alpha -beta and -gamma abolished these inductive events. Furthermore, elimination of asialo-Gm-1+ cells reduced the responses of NK and macrophages to 8-BrGuo, indicating that these cells possibly produce the IFN. We conclude from these observations that one biologic effect of 8-BrGuo is due to IFN production.
D000818:Animals; D001212:Asialoglycoproteins / Q000032:analysis; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D006151:Guanosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D007111:Immunity, Cellular / Q000187:drug effects; D007113:Immunity, Innate / Q000187:drug effects; D066298:In Vitro Techniques; D007372:Interferons / Q000096:biosynthesis / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000276:immunology*; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology; D011070:Poly I-C / Q000494:pharmacology; D013154:Spleen / Q000166:cytology
D001212:Asialoglycoproteins; D006151:Guanosine; C035169:8-bromoguanosine; D007372:Interferons; D011070:Poly I-C