[Detection and differentiation of autoantibodies to extractable nuclear antigens with immunoenzyme tests].
Nuclear antigens were extracted from calf thymus with phosphate-buffered saline The 60-80% ammonium sulfate fraction (ASF) of thymus extract contained SS-B-, the 30-60% ASF Sm- and U1-RNP-, and the chromatographically purified 30-60% ASF only Sm antigen. With these fractions enzyme immunoassays were developed for quantitative determination of Sm-, U1-RNP-, and SS-B autoantibodies. Out of 144 sera with antinuclear antibodies 85% were positive in the enzyme immunoassays, 41% in immunofluorescence tests on liver sections (12% speckled, 29% homogeneous immunofluorescence pattern), and 10% in Ouchterlony tests. 26% of the sera were positive in enzyme immunoassays and immunofluorescence tests. All antibody specificities detected by immunodiffusion could be confirmed by enzyme immunoassay. The enzyme immunoassay is far more sensitive then immunodiffusion and in contrast to immunofluorescence allows antibody specificities to be determined. Enzyme immunoassays are recommended for the diagnosis of rheumatic diseases.
D000974:Antibodies, Antinuclear / Q000032:analysis*; D000918:Antibody Specificity; D034961:Antigens, Nuclear; D001323:Autoantibodies / Q000032:analysis*; D001324:Autoantigens / Q000276:immunology; D003095:Collagen Diseases / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D009687:Nuclear Proteins / Q000276:immunology*; D012261:Ribonucleoproteins / Q000276:immunology; D017411:Ribonucleoproteins, Small Nuclear; D055517:snRNP Core Proteins; D000097585:SS-B Antigen
D000974:Antibodies, Antinuclear; D034961:Antigens, Nuclear; D001323:Autoantibodies; D001324:Autoantigens; D000939:Epitopes; D009687:Nuclear Proteins; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear; D055517:snRNP Core Proteins; D000097585:SS-B Antigen
Untersuchungen zum Nachweis und zur Differenzierung von Autoantikörpern gegen extrahierbare Kernantigene mit Enzymimmuntesten.