The influence of a water-in-oil emulsion on humoral immunity.
A strategy for research of immunostimulants is best served choosing a way by which the immune response can be studied without being complicated by a combination of effects originating both in adjuvant and antigen. This means that adjuvant and antigen preferably are applied with an interval and by different routes. In our model an adjuvant (a W/O emulsion) was applied intraperitoneally, whereas the antigen was injected intravenously. The stimulatory effect on the splenic plaque forming B-cell response depended on the dose of antigen, on the interval between adjuvant and antigen application, on the mouse strain used, and on the quality of the antigen with respect to the intrinsic adjuvanticity of the antigens.
D000276:Adjuvants, Immunologic / Q000008:administration & dosage* / Q000494:pharmacology; D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology*; D004140:Dinitrophenols; D004655:Emulsions; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006241:Haptens; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D005719:gamma-Globulins / Q000276:immunology
D000276:Adjuvants, Immunologic; D000941:Antigens; D004140:Dinitrophenols; D004655:Emulsions; D006241:Haptens; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D005719:gamma-Globulins