Immunochemical approaches to gene probe assays.
Antibodies specific for helical nucleic acids can be applied to assays for hybridized DNA and/or RNA. The assays can use either radioactive or nonradioactive detection systems. Antibodies specific for RNA-DNA hybrids are applicable to assays for measuring hybrid helices that are immobilized on plastic or nitrocellulose, whether the helices are preformed in solution or are formed on the solid-phase support. Alternatively, anti-RNA-DNA hybrid antibodies can be immobilized and used to capture hybrids formed in solution, resulting in an assay with a high signal-to-noise ratio. It has been applied to a test for the presence of ribosomal RNA of Campylobacter jejuni in biological samples.
D002167:Campylobacter / Q000032:analysis; D004247:DNA / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000276:immunology; D011071:Poly T / Q000276:immunology; D012313:RNA / Q000032:analysis*; D012329:RNA, Bacterial / Q000032:analysis
D012329:RNA, Bacterial; D011061:Poly A; D011071:Poly T; C008950:poly A-T; D012313:RNA; D004247:DNA