Isolation of the nuclear gene encoding a subunit of the yeast mitochondrial RNA polymerase.
Antisera directed against the purified 145,000-dalton subunit of the Saccharomyces cerevisiae mitochondrial RNA polymerase have been used to immuno-screen a library of yeast genomic inserts constructed in the fusion protein expression vector, lambda gt11. A 4-kilobase pair yeast DNA fragment inserted into one of the recombinant bacteriophages appears to contain most or all of the gene that encodes the 145,000-dalton subunit.
D010582:Bacteriophage lambda / Q000235:genetics; D003101:Collodion; D004271:DNA, Fungal / Q000302:isolation & purification*; D004274:DNA, Recombinant / Q000302:isolation & purification; D012321:DNA-Directed RNA Polymerases / Q000235:genetics* / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D007106:Immune Sera; D007158:Immunologic Techniques; D008928:Mitochondria / Q000201:enzymology*; D009693:Nucleic Acid Hybridization; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics
D004271:DNA, Fungal; D004274:DNA, Recombinant; D000939:Epitopes; D007106:Immune Sera; D003101:Collodion; D012321:DNA-Directed RNA Polymerases