Antigenic determinants of lipid A analyzed with synthetic models and monoclonal antibodies.
Three monoclonal antibodies directed against the hydrophobic region (lipid A backbone) of Bordetella pertussis endotoxin were used to analyze cross reactivities of lipid A and synthetic glycolipids structurally related to it. Reactivity of mAb with synthetic compounds was measured by a radioimmunoassay inhibition test. Our data indicate that 3-acyloxyacyl groups do not play a significant role, and suggest that a phosphate and a hydroxyl group, at two carbon distance from each other, are part of a single antigenic determinant recognized by the monoclonal antibodies.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity; D000918:Antibody Specificity; D001667:Binding, Competitive; D001886:Bordetella pertussis / Q000276:immunology*; D000939:Epitopes; D006017:Glycolipids / Q000138:chemical synthesis / Q000276:immunology; D008050:Lipid A / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006017:Glycolipids; D008050:Lipid A