The major protein of bull seminal plasma is a secretory product of seminal vesicle.
We isolated the major protein with apparent molecular weight, Mr, 15,000-16,000 from seminal plasma as well as from seminal vesicle secretion of bull and proved by amino acid analysis and tryptic peptide mapping that the two proteins were identical. An antiserum against this major protein was employed to quantitate and identify the major protein in seminal plasma as well as in seminal vesicle secretion. The antiserum did not cross-react with proteins from bovine or human plasma or follicular fluid, respectively. Cell-free translation of poly(A+)RNA isolated from seminal vesicle tissue resulted in formation of one major species with apparent Mr 18,000. Using the anti-major protein antiserum, this major species was specifically immuno absorbed. We thus provided evidence that the major protein component of bull seminal plasma is a secretory protein of seminal vesicles. Furthermore, it appeared that the isolated major protein may be closely related to the protein PDC109, purified from bull seminal plasma and sequenced by Esch et al. (Biochem. Biophys. Res. Commun. 113, 861-867 (1983).
D000818:Animals; D002417:Cattle; D002474:Cell-Free System; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D007120:Immunochemistry; D008297:Male; D011061:Poly A / Q000302:isolation & purification; D029584:Prostatic Secretory Proteins; D014176:Protein Biosynthesis; D011506:Proteins / Q000302:isolation & purification*; D012313:RNA / Q000302:isolation & purification; D012333:RNA, Messenger; D012661:Semen / Q000032:analysis; D029589:Seminal Plasma Proteins; D012669:Seminal Vesicles / Q000378:metabolism*
D029584:Prostatic Secretory Proteins; D011506:Proteins; D012333:RNA, Messenger; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein; D011061:Poly A; D012313:RNA