title: Histologic diagnosis of Hirschsprung's disease. The value of concurrent hematoxylin and eosin and cholinesterase staining of rectal biopsies.
abstract: False positive and negative results can complicate the diagnosis of Hirschsprung's disease (HD) with the acetylcholinesterase (AChE) stain. To improve the diagnostic value of this test, the authors evaluated the concurrent hematoxylin and eosin (H and E) staining of extra sections after the AChE procedure. Flash-frozen (FF), cryostat-cut (CC) sections of rectal suction biopsies from 96 patients with constipation were evaluated by AChE together with H and E staining of additional unstained sections. In 13 of 15 cases of HD with a diagnostic (positive-A) AChE pattern, the H and E sections confirmed the diagnosis. In five cases with other AChE patterns, the H and E sections were instrumental when the diagnosis was made. Of the 76 non-HD subjects with positive-B (n = 8), equivocal (n = 6), and negative (n = 62) AChE patterns, the H and E sections eliminated the diagnosis in 62 (81%). Neuronal and nerve fiber morphologic characteristics were excellent. Rebiopsies were needed in 14 subjects (19%) when there was failure in finding neurons. Simplicity, quickness, and the high quality of the histologic preparations make this procedure a useful adjunct to the AChE stain.
mesh_terms: D000110:Acetylcholinesterase / Q000032:analysis; D000293:Adolescent; D001706:Biopsy; D002648:Child; D002675:Child, Preschool; D003248:Constipation / Q000175:diagnosis / Q000209:etiology; D004801:Eosine Yellowish-(YS); D005629:Frozen Sections; D006416:Hematoxylin; D006627:Hirschsprung Disease / Q000175:diagnosis* / Q000473:pathology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007413:Intestinal Mucosa / Q000294:innervation / Q000473:pathology; D012007:Rectum / Q000294:innervation / Q000473:pathology*; D013194:Staining and Labeling
chemical_list: D000110:Acetylcholinesterase; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin