Diploid and tetraploid precursors of megakaryocytes in normal human bone marrow detected by immunofluorescence.
A sequential preparation method is described which allows immunological identification, morphological characterization, cytophotometric determination of relative DNA content of the megakaryocyte lineage as well as quantitation of megakaryocyte precursors in human bone marrow aspirates. We compared several monoclonal (anti-GP IIIa and HD 19) and polyclonal (A225, RAHPS) antiplatelet antibodies for immunofluorescent staining. Among the identified cells, a small number of cells showing a diploid and tetraploid DNA content were found which must be regarded as promegakaryoblasts, representing 2.5-4.7% of all megakaryocytes. The heterogenous morphology of these precursors in panoptically stained smears is described.
D001854:Bone Marrow Cells; D004247:DNA / Q000378:metabolism; D004171:Diploidy; D005455:Fluorescent Antibody Technique; D006801:Humans; D008533:Megakaryocytes / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D011003:Ploidies; D013194:Staining and Labeling
D004247:DNA