Effects of 2,2'-O-cyclocytidine and acyclovir on latent herpes simplex virus in trigeminal ganglia of mice.
The effects of 2,2'-O-cyclocytidine (CC) and acyclovir (ACV) on latent herpes simplex virus (HSV) in trigeminal ganglia were studied in an in vitro model using reactivation of HSV type 1 (HSV-1) as a model. It was shown that both CC (10 micrograms/ml) and ACV (2.5 micrograms/ml) significantly inhibited the reactivation of the latent HSV-1 in infected ganglia. The effect of CC (25 micrograms/ml), which was as good as that of ACV (10 micrograms/ml), did not last as long as that of ACV after removal of the drugs. The latent state of HSV-1 in vitro was dependent on the continuous presence of either drug. Even though the latent HSV-1 could not be eliminated completely from the trigeminal ganglia by discontinuous administration of either drug, its titers were markedly reduced. The combination of CC and ACV had a synergistic effect on preventing the reactivation of the latent HSV-1 in vitro.
D000212:Acyclovir / Q000494:pharmacology / Q000627:therapeutic use*; D003504:Ancitabine / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D046508:Culture Techniques; D003561:Cytarabine / Q000031:analogs & derivatives*; D004359:Drug Therapy, Combination; D006561:Herpes Simplex / Q000188:drug therapy* / Q000382:microbiology; D051379:Mice; D008815:Mice, Inbred Strains; D018139:Simplexvirus / Q000187:drug effects* / Q000502:physiology; D013997:Time Factors; D012668:Trigeminal Ganglion / Q000382:microbiology*; D014276:Trigeminal Nerve / Q000382:microbiology*; D014779:Virus Replication / Q000187:drug effects
D003561:Cytarabine; D003504:Ancitabine; D000212:Acyclovir