Plasminogen activator inhibitors from placenta and fibrosarcoma cells are antigenically different as evaluated with monoclonal and polyclonal antibodies.
Plasminogen activator inhibitor (PAI) purified from human placenta was compared to PAI purified from conditioned cell culture fluid of the human fibrosarcoma cell line HT-1080. The two inhibitors had a similar mobility (Mr approximately 50,000) in sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Purified placental inhibitor revealed 2 major and 1 minor Coomassie blue stainable bands, while the fibrosarcoma inhibitor appeared as one band. By immunoblotting analysis both monoclonal and polyclonal antibodies against each of the inhibitors showed reaction with the inhibitor against which they were raised, but not cross reaction with the other inhibitor. Similar results were obtained, when antibody binding was tested by ELISA with the inhibitors coated on the solid phase. HPLC fingerprint patterns of cyanogen bromide fragments of the two inhibitors were different. The inhibitory activity of the placental PAI was decreased by a factor of 3 after incubation with SDS, while that of the fibrosarcoma PAI was increased by a factor of 30. It is concluded that the two inhibitors show no detectable common antigenic determinants and most likely are products of different genes.
D000906:Antibodies; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D001666:Binding Sites, Antibody; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D005260:Female; D005354:Fibrosarcoma / Q000032:analysis* / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D010209:Paper; D010920:Placenta / Q000032:analysis* / Q000276:immunology; D015849:Plasminogen Inactivators; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000939:Epitopes; D006023:Glycoproteins; D015849:Plasminogen Inactivators; D012967:Sodium Dodecyl Sulfate; D003101:Collodion