Regulation of lipogenesis in mitogen-stimulated human lymphocytes by thyroid hormones.
The hormonal regulation of precursor incorporation into cellular lipids has been investigated in human lymphocytes stimulated with phytohemeagglutinine. Addition of thyroxine (5 micrograms/ml) for 72 h increased incorporation of [14C]acetate into the triacylglycerol fraction to 290% above the hormone-free control values. Incorporation into the cholesterol fraction was elevated up to 188% under the same conditions. Triiodothyronine was less effective than thyroxine: maximal effects were 153% of the control for triacylglycerols and 142% for cholesterol. Similar results were obtained when [14C]palmitic acid was used as a precursor for triacylglycerol synthesis. Effects of insulin on the parameters described were less pronounced than those obtained with thyroid hormones. Cellular triacylglycerol and protein contents were not elevated significantly by thyroid hormone addition. Further, incorporation of labelled thymidine, uridine, and leucine into acid-precipitable products was not elevated by triiodothyronine above mitogen-stimulated levels. It is concluded, that rapidly dividing lymphocytes provide a suitable system for studies concerning human lipid metabolism.
D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D002250:Carbon Radioisotopes; D004247:DNA / Q000096:biosynthesis; D007328:Insulin / Q000494:pharmacology / Q000502:physiology; D007930:Leucine / Q000378:metabolism; D050356:Lipid Metabolism; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D010835:Phytohemagglutinins / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis; D013936:Thymidine / Q000378:metabolism; D013963:Thyroid Hormones / Q000494:pharmacology* / Q000502:physiology; D014284:Triiodothyronine / Q000494:pharmacology; D014498:Uracil / Q000378:metabolism
D000085:Acetates; D002250:Carbon Radioisotopes; D007328:Insulin; D010169:Palmitic Acids; D010835:Phytohemagglutinins; D013963:Thyroid Hormones; D014284:Triiodothyronine; D019308:Palmitic Acid; D014498:Uracil; D012313:RNA; D004247:DNA; D007930:Leucine; D019342:Acetic Acid; D013936:Thymidine