title: Calcium-permeable channel in sarcoplasmic reticulum of rabbit skeletal muscle.
abstract: Membrane vesicles from sarcoplasmic reticulum of rabbit skeletal muscle were incorporated into a bilayer lipid membrane. With this system, single current fluctuation was observed in the presence of 50 mM Ba-gluconate. This channel activity was observed only in vesicles from terminal cisternae. The single channel conductance was 14.1 pS, and the channel state was almost wholly open. The open-close transition of the channel obeyed simple two-state kinetics and was voltage-independent. The ionic selectivity was also studied, and the channel showed no selectivity among Ba, Ca, Mn, and Mg. On the other hand, it was less permeable to Cs than to Ba. Based on these results, the relation of the Ca channel to excitation-contraction coupling is discussed.
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004553:Electric Conductivity; D007473:Ion Channels / Q000502:physiology*; D008051:Lipid Bilayers; D008564:Membrane Potentials; D009132:Muscles / Q000502:physiology*; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000502:physiology*
chemical_list: D007473:Ion Channels; D008051:Lipid Bilayers; D002118:Calcium