title: Intragastric feeding differentially affects apparent rate of gastric emptying of phenol red and carbohydrate.
abstract: The effects of intragastric feeding on subsequent gastric emptying of glucose and glucose polymer solutions were investigated in rats having chronic gastric catheters. Rats were tested by a modification of the serial test meal method of Hunt. Six ml of solution was fed by mouth or gastric catheter and stomach contents were drained 15 to 60 minutes later. Dilution of stomach contents by gastric secretions and the fluid used to rinse the stomach was assessed by measurement of phenol red and direct assay of the carbohydrate. These two indicators gave very different results. Intragastric feeding of severely food deprived rats appeared to accelerate gastric emptying 30 to 60 minutes after feeding when phenol red was used but not when carbohydrate was used as a marker. Intragastric feeding did not significantly affect the apparent rate of gastric emptying in this same 30-60 minute period in mildly food deprived rats. Even though phenol red and glucose usually give similar results, it is probably inappropriate to estimate the emptying of glucose solely on the basis of measurements of phenol red. When carbohydrate was used as a marker, the chief effect of intragastric feeding is to produce some dumping of gastric contents within 15 minutes after the beginning of intragastric infusion.
mesh_terms: D000818:Animals; D004040:Dietary Carbohydrates; D002149:Energy Intake; D005746:Gastric Emptying; D005766:Gastrointestinal Contents; D005936:Glucans; D005947:Glucose; D008297:Male; D010635:Phenolphthaleins; D010637:Phenolsulfonphthalein; D051381:Rats; D013194:Staining and Labeling
chemical_list: D004040:Dietary Carbohydrates; D005936:Glucans; D010635:Phenolphthaleins; D010637:Phenolsulfonphthalein; D005947:Glucose