Preparation procedure for cerebrospinal fluid that yields cytologic samples suitable for all types of staining, including immunologic and enzymatic methods.
A new method for the cytologic investigation of the cerebrospinal fluid is described. Preparation of the cells is done by successive centrifugation, resuspension of the cells in a protein-enriched medium and cytocentrifugation. The method provided both good cell yields and morphologic preservation. All stainings, including enzymecytochemical and immunocytochemical, could be performed on the specimens. The cell-free fluid from the first centrifugation is also available for use in other studies.
D002469:Cell Separation / Q000379:methods*; D002555:Cerebrospinal Fluid / Q000166:cytology*; D006651:Histocytochemistry; D006801:Humans; D007124:Immunoenzyme Techniques; D007159:Immunologic Tests; D013194:Staining and Labeling