Localization of a novel v-erbB-related gene, c-erbB-2, on human chromosome 17 and its amplification in a gastric cancer cell line.
The c-erbB-2 gene is a v-erbB-related proto-oncogene which is distinct from the gene encoding the epidermal growth factor receptor. By using two independent methods, hybridization of both sorted chromosomes and metaphase spreads with cloned c-erbB-2 DNA, we mapped the c-erbB-2 locus on human chromosome 17 at q21, a specific breakpoint observed in a translocation associated with acute promyelocytic leukemia. Furthermore, we observed amplification and elevated expression of the c-erbB-2 gene in the MKN-7 gastric cancer cell line. These data suggest possible involvement of the c-erbB-2 gene in human cancer.
D002460:Cell Line; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D005784:Gene Amplification; D006801:Humans; D007621:Karyotyping; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000235:genetics; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D013274:Stomach Neoplasms / Q000235:genetics*; D014158:Transcription, Genetic
C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA