Dual expression of the cell-surface antigens HLA-DR and CD1 (T6) by Langerhans cells in human buccal mucosa and skin.
The expression of HLA-DR and CD1 (T6) by Langerhans cells (LC) in human buccal mucosa and skin was investigated with the monoclonal antibodies YE2/36HLK (HLA-DR) and HTA1-C1 (CD1). A five-stage sequential double immunofluorescent-labelling technique, with rhodamine and fluorescein as the fluorochromes, was used to visualize the two surface antigens in the same microscope field. The majority of LC in cryostat sections of buccal mucosa and skin expressed both HLA-DR and CD1.
D000911:Antibodies, Monoclonal; D018949:Antigens, CD1; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology*; D002610:Cheek; D005455:Fluorescent Antibody Technique; D006681:HLA-D Antigens / Q000276:immunology*; D006684:HLA-DR Antigens / Q000276:immunology*; D006801:Humans; D007801:Langerhans Cells / Q000276:immunology*; D009061:Mouth Mucosa / Q000166:cytology* / Q000276:immunology; D012867:Skin / Q000276:immunology*
D000911:Antibodies, Monoclonal; D018949:Antigens, CD1; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D006681:HLA-D Antigens; D006684:HLA-DR Antigens