Murine monoclonal antibodies associated with Rh17, Rh29, and Rh46 antigens.
After immunization with native human red cells and red cells infected with Plasmodium falciparum, mouse splenocytes were fused with a myeloma cell line to obtain hybridomas. Among the antibodies specific for blood group antigens, four antibodies (iB3C4, F12, MR432, and iB4) directed against epitopes related to the Rh antigen were selected for characterization. It is suggested that two monoclonal antibodies (iB3C4 and F12) recognized an epitope associated with the Rh29 antigen. The specificity of the monoclonal antibody iB4 seemed related to the CcEe series of antigens. MR432 did not react with red cells of people who are homozygous for the RN gene, and seemed to identify an epitope associated with the Rh46 antigen (recently numbered).
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000939:Epitopes / Q000032:analysis; D006384:Hemagglutination; D006801:Humans; D051379:Mice; D012204:Rh-Hr Blood-Group System / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D012204:Rh-Hr Blood-Group System