Separation and identification of methotrexate and its metabolites, 7-hydroxymethotrexate and polyglutamates, in human tissues by reversed-phase high-performance liquid chromatography coupled with radioimmunoassay.
D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008099:Liver / Q000032:analysis; D008727:Methotrexate / Q000031:analogs & derivatives* / Q000302:isolation & purification* / Q000652:urine; D010455:Peptides / Q000302:isolation & purification*; D011099:Polyglutamic Acid / Q000302:isolation & purification*; D011863:Radioimmunoassay; D013056:Spectrophotometry, Ultraviolet
D010455:Peptides; D011099:Polyglutamic Acid; C011864:7-hydroxymethotrexate; D008727:Methotrexate