Blast colonies containing hemopoietic progenitor cells can give rise to Abelson virus (A-MuLV)-transformed cell lines.
We have established an in vitro system with which to examine the ability of Abelson murine leukemia virus (A-MuLV) to infect early hemopoietic progenitor cells. Blast cell colonies containing less than 100 cells were shown to contain up to 85% of cells with secondary hemopoietic colony-forming ability. Infection of cells from these blast colonies resulted in generation of transformed mast cell lines when a feeder was provided. Morphological examination of cells taken from infected cultures at various times postinfection indicated a progression of cellular differentiation to the mast cell lineage. Southern analysis on early subclones of transformed cells from two wells, using a v-abl specific probe, indicated a unique pattern of viral integration amongst subclones, suggesting that all subclones had derived from a single cell in each well. Similar results were observed with helper-free Abelson virus obtained by transfecting psi 2 cells with P160 A-MuLV proviral DNA. These data indicate that hemopoietic progenitor cells can be infected by A-MuLV and subsequently in our in vitro culture condition give rise to transformed mast cell lines.
D000011:Abelson murine leukemia virus / Q000254:growth & development*; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D002472:Cell Transformation, Viral; D006378:Helper Viruses / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000382:microbiology*; D009052:Leukemia Virus, Murine / Q000254:growth & development*; D008407:Mast Cells / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013997:Time Factors
D012194:RNA-Directed DNA Polymerase