Re-examination and further characterization of a monoclonal antibody to hepatitis B e antigen (anti-HBe).
A purification procedure for serum hepatitis B e antigen (HBeAg) was developed to immunize mice for monoclonal anti-HBe production. Two monoclonal anti-HBe secreting hybridomas were identified. Immunoglobulin G (IgG2a) was isolated from each hybridoma and labeled with either 125I or horseradish peroxidase. Each label was used as a probe in solid phase immunoassays for HBeAg and anti-HBe detection. Both monoclonal antibodies recognized the beta epitope on HBeAg, but one consistently performed better as a probe. When this monoclonal probe was compared to commercially available polyclonal assays, it showed equivalent sensitivity and specificity.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000939:Epitopes / Q000276:immunology; D005260:Female; D006510:Hepatitis B Antibodies / Q000032:analysis* / Q000276:immunology; D006511:Hepatitis B Antigens / Q000276:immunology*; D006513:Hepatitis B e Antigens / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006510:Hepatitis B Antibodies; D006511:Hepatitis B Antigens; D006513:Hepatitis B e Antigens; D007074:Immunoglobulin G