A reliable technique for marking the location of extracellular recording sites using glass micropipettes.
A simple and highly reliable technique is described for marking the locations of extracellularly recorded neurons using double-barreled glass micropipettes. One barrel contains 3 M NaCl for recording; the other contains horseradish peroxidase (HRP) that is iontophoretically ejected using low currents. With appropriate processing of the tissue, small well-localized spots are produced that contain a small number of HRP-filled neurons. The technique is effective in marking sequential recording sites within individual electrode tracks and in multiple penetrations using the same microelectrode.
D000200:Action Potentials; D000818:Animals; D001370:Axonal Transport; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods; D005898:Glass; D006735:Horseradish Peroxidase; D008839:Microelectrodes; D009474:Neurons / Q000166:cytology / Q000502:physiology*
D006735:Horseradish Peroxidase