Preparative high-performance liquid chromatography of proteins on an anion exchanger using unfractionated carboxymethyldextran displacers.
It has been previously demonstrated that carboxymethyldextran (CMD) displacers fractionated on an ion-exchange column can be used for the resolution and high capacity displacement chromatography of proteins on high-performance ion-exchange columns. It is shown, in this manuscript, that high resolution and high capacity are obtainable using unfractionated CMD displacers. Up to 25 mg of a mixture of ovalbumin, alpha-lactalbumin, and soy-bean trypsin inhibitor along with many impurities were separated on a 3.3-ml Altex DEAE-5PW column using three unfractionated CMD displacers and a final CMD displacer. A method for removing the displacers from proteins, on a hydrophobic interaction column, is also described.
D000837:Anion Exchange Resins; D002848:Chromatography, DEAE-Cellulose; D002851:Chromatography, High Pressure Liquid; D003911:Dextrans; D004591:Electrophoresis, Polyacrylamide Gel; D007768:Lactalbumin / Q000032:analysis; D010047:Ovalbumin / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000032:analysis
D000837:Anion Exchange Resins; D003911:Dextrans; D011506:Proteins; D010047:Ovalbumin; D007768:Lactalbumin; C014392:carboxymethyl dextran; D014360:Trypsin Inhibitor, Kunitz Soybean