[Preparation and characterization of specific antisera directed against different polypeptide domains encoded by the c-myc oncogene for studying the expression of this gene introduced into quail or rat cells].
By using bacterial expression vectors, we have prepared antisera directed against two polypeptidic domains encoded by exons 2 and 3 of the human c-myc oncogene. These antisera which detect specifically the human c-myc proteins allow us to analyse the expression of human c-myc gene activated by retroviral sequences and introduced in quail embryo cells (QEC) or in established rat embryo fibroblastic cell line (208 F). Although human myc mRNA are expressed in the two cell types, the p64/p67 human c-myc proteins are only detected in the QEC.
D000818:Animals; D002471:Cell Transformation, Neoplastic; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism; D000939:Epitopes / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D006801:Humans; D007106:Immune Sera; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes; D011784:Quail; D051381:Rats
D004274:DNA, Recombinant; D000939:Epitopes; D007106:Immune Sera; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D004262:DNA Restriction Enzymes
Préparation et caractérisation d'antisera spécifiques dirigés contre différents domaines polypeptidiques codés par l'oncogène c-myc humain pour étudier l'expression de ce gène introduit dans des cellules de caille ou de rat.