Regulation by serine esterase of histamine release from human leukocytes--I. Direct release of histamine by the serine esterase inhibitors diisopropyl fluorophosphate (DFP) and soman (GD).
The serine esterase inhibitor diisopropyl fluorophosphate (DFP) had been reported previously to inhibit IgE-dependent histamine release. Recently, it has been demonstrated that lower concentrations of DFP enhance IgE-dependent histamine release and inhibit desensitization. This manuscript describes the abilities of several esterase inhibitors to cause release of histamine from human leukocytes (basophils), by a process that is IgE-independent. This esterase inhibitor-induced histamine release appears to be by a non-cytotoxic mechanism that requires calcium and is temperature dependent. These histamine release processes occurred over a longer period of time than IgE-dependent release. Direct release of histamine by these small molecular weight inhibitors and inhibition of desensitization both suggest that one or more serine esterases are involved in the regulation of histamine release from human basophils.
D004492:Edetic Acid / Q000494:pharmacology; D004950:Esterases / Q000037:antagonists & inhibitors / Q000502:physiology*; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D066298:In Vitro Techniques; D007531:Isoflurophate / Q000494:pharmacology*; D007700:Kinetics; D007962:Leukocytes / Q000378:metabolism*; D009243:NAD / Q000032:analysis; D012999:Soman / Q000494:pharmacology*
D009243:NAD; D007531:Isoflurophate; D007073:Immunoglobulin E; D012999:Soman; D004492:Edetic Acid; D004950:Esterases; C022635:serine esterase