Substrate specificity of fucosyltransferase purified from human parotid saliva.
The purified fucosyltransferase from human parotid saliva was shown to transfer fucose from GDP-fucose onto the oligosaccharide chains containing the Gal beta 1----3GlcNAc or Gal beta 1----4GlcNAc/Glc sequences. Competition studies between asialotransferrin and either lacto-N-fucopentaose 1 or 2'-fucosyllactose provided evidence that both the substrates competed for a common enzyme active site. These results suggest that the fucosyltransferase activities for the three acceptors may be catalyzed by the same enzyme.
D000117:Acetylglucosamine / Q000378:metabolism; D001212:Asialoglycoproteins; D060748:Fetuins; D005647:Fucosyltransferases / Q000378:metabolism*; D006602:Hexosyltransferases / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D009844:Oligosaccharides / Q000378:metabolism; D010306:Parotid Gland / Q000201:enzymology*; D010455:Peptides / Q000378:metabolism; D055232:Proline-Rich Protein Domains; D012463:Saliva / Q000201:enzymology*; D012471:Salivary Proteins and Peptides / Q000378:metabolism; D013379:Substrate Specificity; D014168:Transferrin / Q000031:analogs & derivatives / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000378:metabolism
D001212:Asialoglycoproteins; D060748:Fetuins; D009844:Oligosaccharides; D010455:Peptides; D012471:Salivary Proteins and Peptides; D014168:Transferrin; D000509:alpha-Fetoproteins; C017029:asialofetuin; C017031:asialotransferrins; D005647:Fucosyltransferases; D006602:Hexosyltransferases; D000117:Acetylglucosamine