Hybridoma cell lines secreting monoclonal antibodies against equine infectious anemia virus.
A monoclonal anti-equine infectious anemia virus (anti-EIAV) antibody (1B15) has been generated by fusion of X63 Ag 8.653 myeloma cells and spleen cells from mice hypersensitized with viral antigen p29. Ouchterlony double-diffusion analysis indicated that antibody 1B15 is of the IgG class. The specificity of the immune reaction for p29 was confirmed by cross-over immunoelectrophoresis and disc-gel electrophoresis. MAb 1B15 was used to devise a solid-phase 'capture' RIA for EIAV-p29 antigen. The antigen, bound by 1B15 adsorbed onto wells of flexible microtitre plates, was detected using a rabbit anti-p29 serum followed by a 125I-labelled tracer. The assay was applied to detected using a of virus in horse serum and infected cell culture fluids.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D003378:Counterimmunoelectrophoresis; D004589:Electrophoresis, Disc; D000939:Epitopes; D004859:Equine Infectious Anemia / Q000175:diagnosis; D006736:Horses; D006825:Hybridomas; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology; D004860:Infectious Anemia Virus, Equine / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011863:Radioimmunoassay; D014764:Viral Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D007074:Immunoglobulin G; D014764:Viral Proteins