title: gp140v-fms molecules expressed at the surface of cells transformed by the McDonough strain of feline sarcoma virus are phosphorylated in tyrosine and serine.
abstract: Cells transformed by the McDonough strain of feline sarcoma virus express at their surface a v-fms-specific transmembrane glycoprotein designated gp140v-fms. By labeling with 32Pi, gp140v-fms was shown to be phosphorylated 30-fold more in serine residues than were the cytosolic v-fms polypeptides gp180gag-fms and gp120v-fms. By using the phosphotyrosine phosphatase-specific inhibitor sodium orthovanadate, an additional tyrosine phosphorylation was observed in vivo, again involving predominantly gp140v-fms. In vitro studies showed that the v-fms proteins were phosphorylated by protein kinase C in a calcium- and phosphatidylserine-dependent manner.
mesh_terms: D000818:Animals; D002415:Cats; D002471:Cell Transformation, Neoplastic; D005796:Genes; D005814:Genes, Viral; D016281:Oncogene Protein gp140(v-fms); D010766:Phosphorylation; D010768:Phosphoserine / Q000032:analysis*; D019000:Phosphotyrosine; D011493:Protein Kinase C / Q000378:metabolism; D012190:Retroviridae / Q000187:drug effects / Q000235:genetics*; D012191:Retroviridae Proteins / Q000235:genetics* / Q000302:isolation & purification; D012694:Serine / Q000031:analogs & derivatives*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis; D014638:Vanadates; D014639:Vanadium / Q000494:pharmacology
chemical_list: D016281:Oncogene Protein gp140(v-fms); D012191:Retroviridae Proteins; D014639:Vanadium; D010768:Phosphoserine; D019000:Phosphotyrosine; D014638:Vanadates; D014443:Tyrosine; D012694:Serine; D011493:Protein Kinase C