Inhibition of Rous sarcoma virus replication by antisense RNA.
Previous results have indicated that Rous sarcoma virus env gene expression is specifically inhibited by antisense RNA (L.-J. Chang and C. M. Stoltzfus, Mol. Cell. Biol. 5:2341-2348, 1985). In this study, we compare the extents of inhibition by antisense RNA derived from different parts of the Rous sarcoma virus genome, and we show that antisense constructs containing the 3'-end noncoding region inhibit env expression to a similar extent as those containing the 5'-end noncoding region or coding region. Furthermore, we show that antisense RNA inhibits virus replication at other levels in addition to translation.
D001358:Avian Sarcoma Viruses / Q000235:genetics*; D001483:Base Sequence; D004274:DNA, Recombinant; D005786:Gene Expression Regulation; D005814:Genes, Viral; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000235:genetics; D014779:Virus Replication
D004274:DNA, Recombinant; D012333:RNA, Messenger; D012367:RNA, Viral; D014759:Viral Envelope Proteins; D012313:RNA