[A new primer for sequencing DNA fragments cloned in M13mp vectors].
Tridecadeoxyribonucleotide d(CCAGGGTTTTCCC) was prepared by solid phase crown-ether-catalyzed phosphotriester method and proposed a new sequencing primer. This primer expands capacities of the Sanger dideoxy-chain-terminating method to solve various sequencing problems as compared to well-known universal primers. Criteria of the choice of an oligonucleotide primer without computer analysis of nucleotide sequence are described.
D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000096:biosynthesis* / Q000235:genetics; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; D005822:Genetic Vectors; D009841:Oligonucleotides / Q000235:genetics; D012313:RNA
D009841:Oligonucleotides; C034477:RNA primers; D012313:RNA; D004247:DNA; D004262:DNA Restriction Enzymes; C046545:CCWGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific
Novyĭ praĭmer dlia sekvenirovaniia fragmentov DNK, klonirovannykh v M13mp-vektorakh.