[Sequences containing the Alu elements from a human fibroblast cDNA clone library: nucleotide sequence and expression in various tissues].
Alu containing cDNA clones were isolated from a human fibroblast cDNA library. The nucleotide sequences of two clones were determined. In both cases Alu repeats appeared to be situated in the regions presumably corresponding to the 3'-end of mRNA. The comparison of the unique sequences of those clones did not show any homology between them. Both cDNA clones contained large open reading frames, extending into the Alu regions. DNA of one clone was used as a probe for Northern blot-hybridization analysis of poly(A)+ cytoplasmic RNA from some normal and tumor human tissues. All RNA samples contained Alu-homologous transcripts, mainly in 7-12S fractions and only in one (canonical) orientation. This orientation contained functional regions including promotor region for RNA-polymerase III.
D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004273:DNA, Neoplasm / Q000235:genetics; D005347:Fibroblasts; D005786:Gene Expression Regulation; D006801:Humans; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012313:RNA / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D014018:Tissue Distribution
D004273:DNA, Neoplasm; D012313:RNA; D004247:DNA
Posledovatel'nosti, soderzhashchie élementy Alu, iz banka klonov kDNK fibroblastov cheloveka: opredelenie nukleotidnoĭ posledovatel'nosti i ékspressiia v raznykh tkaniakh.