Enhanced releasability of prostaglandin E2 and leukotrienes B4 and C4 from leukocytes of patients with atopic eczema.
The releasability of arachidonic acid-derived inflammatory mediators (eicosanoids) from peripheral blood leukocytes has been tested in patients with atopic eczema and healthy, non-atopic controls. Spontaneous and stimulated release of prostaglandin E2 (PGE2), leukotriene B4 (LTB4) and leukotriene C4 (LTC4) has been measured after challenge of cells with various concentrations of anti-IgE, Ca-ionophore A23187 and C5a. The maximal stimulation of cells with Ca-ionophore resulted in the generation of high amounts of all eicosanoids, which was essentially equal in both atopic and control groups. On the other hand, enhanced spontaneous and stimulated eicosanoid release was noted after immunological challenge using C5a and anti-IgE in the atopic eczema group. Thus, our data support the hypothesis of enhanced releasability of inflammatory mediators in atopic eczema.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology; D001095:Arachidonic Acids / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002648:Child; D003182:Complement C5 / Q000494:pharmacology; D015936:Complement C5a; D003876:Dermatitis, Atopic / Q000276:immunology*; D015232:Dinoprostone; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D007962:Leukocytes / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D007975:Leukotriene B4 / Q000096:biosynthesis; D008297:Male; D008875:Middle Aged; D011458:Prostaglandins E / Q000096:biosynthesis; D013189:SRS-A / Q000096:biosynthesis
D000888:Antibodies, Anti-Idiotypic; D001095:Arachidonic Acids; D003182:Complement C5; D011458:Prostaglandins E; D013189:SRS-A; D007975:Leukotriene B4; D007073:Immunoglobulin E; D000001:Calcimycin; D015936:Complement C5a; D015232:Dinoprostone