A physical map of the human regulator of complement activation gene cluster linking the complement genes CR1, CR2, DAF, and C4BP.
We report the organization of the human genes encoding the complement components C4-binding protein (C4BP), C3b/C4b receptor (CR1), decay accelerating factor (DAF), and C3dg receptor (CR2) within the regulator of complement activation (RCA) gene cluster. Using pulsed field gel electrophoresis analysis these genes have been physically linked and aligned as CR1-CR2-DAF-C4BP in an 800-kb DNA segment. The very tight linkage between the CR1 and the C4BP loci, contrasted with the relative long DNA distance between these genes, suggests the existence of mechanisms interfering with recombination within the RCA gene cluster.
D018958:CD55 Antigens; D002874:Chromosome Mapping; D003167:Complement Activation; D003181:Complement C4 / Q000235:genetics* / Q000302:isolation & purification; D015935:Complement C4b; D004587:Electrophoresis, Agar Gel; D008040:Genetic Linkage; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics* / Q000302:isolation & purification; D005810:Multigene Family; D009693:Nucleic Acid Hybridization; D011951:Receptors, Complement / Q000235:genetics* / Q000302:isolation & purification; D017463:Receptors, Complement 3b; D017464:Receptors, Complement 3d
D018958:CD55 Antigens; D003181:Complement C4; D008565:Membrane Proteins; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D017464:Receptors, Complement 3d; C044257:factor H receptors; D015935:Complement C4b