Heterogeneity of presynaptic calcium channels revealed by species differences in the sensitivity of synaptosomal 45Ca entry to omega-conotoxin.
The inhibition of K+-depolarization dependent Ca influx by omega-conotoxin GVIA was compared in the frog, chick, and rat brain synaptosomes. The toxin at concentrations greater than or equal to 0.3 microM completely inhibited Ca entry in the frog and chick preparations, but was only partly effective in blocking Ca influx in the rat brain synaptosomes. In chick synaptosomes the toxin's effect was biphasic: a small component (approximately equal to 15%) of total Ca influx was inhibited by the toxin with high affinity (I50 less than 0.002 microM); a major component (approximately equal to 80%) of Ca influx was inhibited with a moderate affinity (I50 approximately equal to 0.05 microM). In rat brain synaptosomes 40% of Ca influx was inhibited by the toxin with low affinity (I50 approximately equal to 0.3 microM), and 60% of Ca influx was unaffected by the toxin concentration of up to 10 microM. These data suggest a heterogeneity of voltage-sensitive Ca channels in vertebrate brain synaptosomes.
D000818:Animals; D001921:Brain / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002132:Calcium Radioisotopes; D002645:Chickens; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008978:Mollusk Venoms / Q000494:pharmacology*; D011188:Potassium / Q000494:pharmacology; D011894:Rana pipiens; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D013569:Synapses / Q000187:drug effects / Q000378:metabolism*; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*; D020866:omega-Conotoxin GVIA
D002132:Calcium Radioisotopes; D007473:Ion Channels; D008978:Mollusk Venoms; D020866:omega-Conotoxin GVIA; D011188:Potassium; D002118:Calcium