title: [Study of the structural characteristics of the regulatory subunit of cAMP-dependent protein kinase type II using monoclonal antibodies].
abstract: Monoclonal antibodies to the regulatory subunit of cAMP-dependent protein kinase type II from porcine brain were used to study the antigenic properties of the enzyme regulatory subunit (RII). The monoclonal antibodies were bound to linear antigenic determinants on the protein molecule surface. The cAMP binding to RII interfered with the interaction between monoclonal antibodies and the protein. The use of different proteolytic fragments of RII allowed for the localization of antigenic determinants in the N-terminal moiety of RII.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001665:Binding Sites; D001921:Brain / Q000201:enzymology; D000242:Cyclic AMP / Q000378:metabolism; D000939:Epitopes / Q000032:analysis; D007527:Isoenzymes / Q000032:analysis* / Q000276:immunology; D008970:Molecular Weight; D011494:Protein Kinases / Q000032:analysis* / Q000276:immunology; D013552:Swine
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D007527:Isoenzymes; D000242:Cyclic AMP; D011494:Protein Kinases
vernacular_title: Izuchenie strukturnykh osobennosteĭ reguliatornoĭ sub''edinitsy cAMP-zavisimoĭ proteinkinazy tipa II s pomoshch'iu monoklonal'nykh antitel.