Antibody probing of western blots which have been stained with india ink.
India ink can be used to stain proteins bound to nitrocellulose. Subsequently, specific proteins can be identified with antibodies and 125I-protein A. In most cases, india ink did not significantly inhibit detection with antibody, nor was the ink washed off during the antibody incubation steps. Using this method, a direct comparison of antibody-reactive protein and total protein can be made with the same replica.
D000906:Antibodies; D002244:Carbon; D004396:Coloring Agents; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D011506:Proteins / Q000032:analysis*; D013194:Staining and Labeling
D000906:Antibodies; D004396:Coloring Agents; D011506:Proteins; C028433:chinese ink; D002244:Carbon