Isolation of antibodies for phosphotyrosine by immunization with a v-abl oncogene-encoded protein.
Immunization of rabbits with a tyrosine-phosphorylated v-abl protein resulted in the production of antibodies for the v-abl protein and for phosphotyrosine. The antiphosphotyrosine antibodies could be purified by affinity chromatography with O-phosphotyramine coupled to Sepharose. These antibodies detected a variety of tyrosine-phosphorylated proteins, including receptors for peptide growth factors. The usefulness of these antibodies was demonstrated by the detection of previously unidentified tyrosine-phosphorylated proteins in v-src-, v-abl-, and v-erbB-transformed cell lines.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D002471:Cell Transformation, Neoplastic; D002846:Chromatography, Affinity; D007114:Immunization; D009856:Oncogene Proteins, Viral / Q000276:immunology*; D019000:Phosphotyrosine; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000276:immunology
D000906:Antibodies; D009856:Oncogene Proteins, Viral; D019000:Phosphotyrosine; D014443:Tyrosine