Use of a monoclonal antibody (VM-2) plus the immunogold-silver technique to stain basal cell carcinoma cells.
A newly described murine monoclonal antibody, VM-2, stains both basal cell carcinoma cells and squamous cell carcinoma cells of the skin. We have combined this monoclonal antibody with a newly described technique, the immunogold-silver stain, to produce a method for rapid characterization of basal cell carcinoma cells. This method does not require the use of a fluorescence microscope and gives a very intense stain of the tumor cells. Tumor-containing tissue from 12 patients was stained and included 2 cases of morphealike basal cell carcinoma. All the tumors stained with VM-2 gold. Thus, staining with this technique may be helpful in diagnosing tumors of epithelial origin and may also find application in delineating residual tumor cells in microscopically controlled excision.
D000911:Antibodies, Monoclonal; D002280:Basal Cell Carcinoma / Q000175:diagnosis* / Q000473:pathology / Q000601:surgery; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D012878:Skin Neoplasms / Q000175:diagnosis* / Q000473:pathology / Q000601:surgery; D013194:Staining and Labeling / Q000379:methods
D000911:Antibodies, Monoclonal