Simultaneous detection of indoleamines and dopamine in rat dorsal raphe nuclei using specific antibodies.
Using a monoclonal antibody against dopamine and a rabbit antiserum against serotonin, 5-methoxytryptamine or tryptamine, we were able to achieve the simultaneous localization of two amines in glutaraldehyde-fixed sections of rat dorsal raphe nuclei. In this staining procedure, the first antigen was localized using 3,3'-diaminobenzidine (DAB), while the second antigen was stained using the 1-naphthol basic dye (2-NBD) method. The two antigens were localized in different cells or structures. No overlap of the staining was observed, thus indicating that dopamine is not localized with serotonin, 5-methoxytryptamine or tryptamine.
D015100:3,3'-Diaminobenzidine; D008735:5-Methoxytryptamine / Q000032:analysis; D000588:Amines / Q000032:analysis*; D000818:Animals; D000911:Antibodies, Monoclonal; D004396:Coloring Agents; D004298:Dopamine / Q000032:analysis*; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D009284:Naphthols; D011903:Raphe Nuclei / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000032:analysis; D013194:Staining and Labeling; D014363:Tryptamines / Q000032:analysis
D000588:Amines; D000911:Antibodies, Monoclonal; D004396:Coloring Agents; D009284:Naphthols; D014363:Tryptamines; D015100:3,3'-Diaminobenzidine; D012701:Serotonin; D008735:5-Methoxytryptamine; C030820:tryptamine; D004298:Dopamine