Sepharose gels as antigenic matrices for specific binding of anti-galactosyl antibodies.
From 100 ml of pooled normal serum taken from different species (man, cow, rabbit, chicken, duck, carp) 0.5 to 4.0 mg of high and low molecular weight anti-galactosyl antibodies could be isolated by affinity-chromatography on non-ligand-attached Sepharose 4B. The antibodies bound to the agarose matrix were eluted with 0.5 M D-galactose containing buffers. Using species-specific anti-immunoglobulin sera in immunoelectrophoresis, the immunoglobulin character of the protein fractions prepared was analyzed. In addition, the H and L chain structure of the anti-galactosyl antibodies was checked by SDS-PAGE.
D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000302:isolation & purification*; D001666:Binding Sites, Antibody; D002347:Carps / Q000276:immunology; D002417:Cattle; D002645:Chickens / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D007122:Immunoelectrophoresis; D007132:Immunoglobulin Isotypes / Q000032:analysis; D007163:Immunosorbent Techniques; D011817:Rabbits; D012685:Sepharose / Q000276:immunology*; D012967:Sodium Dodecyl Sulfate; D013045:Species Specificity
D000906:Antibodies; D000939:Epitopes; D006023:Glycoproteins; D007132:Immunoglobulin Isotypes; C017336:galactoproteins; D012967:Sodium Dodecyl Sulfate; D012685:Sepharose