Effect of forskolin and phosphodiesterase inhibition on prostacyclin-stimulated steroid production in goat ovarian cell types.
The effect of alteration of cyclic AMP pathway on prostacyclin (PGI2)-stimulated steroid production in goat granulosa (G), theca (TH) and corpus luteum (CL) cells was studied in vitro. Isobutyl methyl xanthine (IBMX, 10(-3) M), a phosphodiesterase inhibitor, stimulated steroid production in all the cell types. IBMX enhanced the effects of PGI2 (1 ug/ml). Forskolin (F, 10(-7) -10(-4) M), an adenyl cyclase stimulator produced dose-dependent increase in progesterone (P4) and estradiol (E2) production in all the three cell types. When combined with IBMX, F2 produced further increase in P4 and E2 production. When F was combined with PGI2 (1 ug/ml) the two produced highly significant (p less than 0.001) synergistic effect on P4 and E2 production in all the three cell types. These findings suggest that the stimulatory action of PGI2 on ovarian steroid production is mediated through stimulation of adenyl cyclase.
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D005576:Colforsin / Q000494:pharmacology*; D003338:Corpus Luteum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D011464:Epoprostenol / Q000494:pharmacology*; D004958:Estradiol / Q000096:biosynthesis*; D005260:Female; D006041:Goats; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism; D010053:Ovary / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D011374:Progesterone / Q000096:biosynthesis*; D013799:Theca Cells / Q000187:drug effects / Q000378:metabolism
D010726:Phosphodiesterase Inhibitors; D005576:Colforsin; D011374:Progesterone; D004958:Estradiol; D011464:Epoprostenol; D015056:1-Methyl-3-isobutylxanthine