[Use of immunospecific components labelled with beta-lactamase and horseradish peroxidase for immunoenzyme analysis].
A system for enzyme immunoassay with the use of beta-lactamase as an enzyme marker is described. Similar sensitivity of the enzyme immunoassays with the use of beta-lactamase and horse-raddish peroxidase as enzyme markers was shown. The data on decrease of the antigen binding capacity of the antibodies in the conjugates with beta-lactamase are presented. It is suggested that such a decrease may be associated with glutaric dialdehyde conjugation. The use of the schemes for enzyme immunoassay with simultaneous application of two independently recorded enzymes i. e. horse-raddish peroxidase and beta-lactamase provided confirmation of univalency of the conjugate of the melted capsular antigen of the plague causative agent with beta-lactamase. Identity of the antigenic determinants of the melted antigen and capsular antigen of the plague microbe was demonstrated.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D000942:Antigens, Bacterial / Q000032:analysis; D001667:Binding, Competitive; D000939:Epitopes / Q000032:analysis; D006735:Horseradish Peroxidase / Q000032:analysis* / Q000276:immunology; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D007202:Indicators and Reagents; D051379:Mice; D008807:Mice, Inbred BALB C; D010544:Peroxidases / Q000032:analysis*; D011817:Rabbits; D015010:Yersinia pestis / Q000276:immunology; D001618:beta-Lactamases / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes; D007202:Indicators and Reagents; D006735:Horseradish Peroxidase; D010544:Peroxidases; D001618:beta-Lactamases
Ispol'zovanie immunospetsificheskikh komponentov, mechennykh beta-laktamazoĭ i peroksidazoĭ khrena dlia immunofermentnogo analiza.