Structural analysis of the major human erythrocyte membrane sialoglycoprotein from Miltenberger class VII cells.
The major human erythrocyte membrane sialoglycoprotein (glycophorin A or MN glycoprotein) was purified from the red blood cells of an individual, homozygous for the Mi-VII gene in the Miltenberger subsystem of the MNSs blood-group system. The complete structure of a tryptic peptide comprising the residues 40-61 of glycophorin A was deduced from manual sequence analyses. The Mi-VII-specific glycophorin A was shown to exhibit an arginine----threonine and a tyrosine----serine exchange at the positions 49 and 52 respectively. The threonine-49 residue was found to be glycosylated. Inhibition assays demonstrated that one of the Mi-VII-specific antigen determinants (Anek) is located within the residues 40-61 of glycophorin A and comprises sialic acid residue(s) attached to O-glycosidically linked oligosaccharide(s). Our data contribute to an understanding of the Miltenberger system and provide an explanation at the molecular level for the previous finding that the erythrocytes from the Mi-VII homozygote lack a high-frequency antigen (EnaKT), located within the residues 46-56 of normal glycophorin A.
D000596:Amino Acids / Q000097:blood; D002241:Carbohydrates / Q000097:blood; D000939:Epitopes / Q000032:analysis; D004910:Erythrocyte Membrane / Q000032:analysis*; D006021:Glycophorins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D006720:Homozygote; D006801:Humans; D008951:MNSs Blood-Group System / Q000235:genetics / Q000276:immunology; D008565:Membrane Proteins / Q000097:blood* / Q000235:genetics / Q000276:immunology; D012795:Sialoglycoproteins / Q000032:analysis*
D000596:Amino Acids; D002241:Carbohydrates; D000939:Epitopes; D006021:Glycophorins; D008951:MNSs Blood-Group System; D008565:Membrane Proteins; D012795:Sialoglycoproteins