Stimulation by interferon of the differentiation of human promyelocytic leukemia (HL-60) cells produced by retinoic acid and actinomycin D.
Interferons (IFNs) have been shown to enhance or induce differentiation in several model systems, either alone or in combination with differentiating agents. To further study these phenomena, the effects of the recombinant interferons rIFN-alpha A, rIFN-alpha D, rIFN-alpha A/D (Bgl II), rIFN-beta, and rIFN-gamma on the differentiation-producing capacities of the anticancer agents aclacinomycin A and actinomycin D, and the differentiating agent retinoic acid, were examined in the HL-60 promyelocytic leukemia cell line. The addition of IFNs to aclacinomycin A did not produce any enhancement of differentiating capacity. The differentiating capacity of actinomycin D was enhanced only by rIFN-alpha A/D (Bgl II). The differentiating capacity of retinoic acid was enhanced by all the IFNs-alpha tested, and by rIFN-beta, but not rIFN-gamma.
D015250:Aclarubicin; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003609:Dactinomycin / Q000494:pharmacology; D004357:Drug Synergism; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy* / Q000473:pathology; D009279:Naphthacenes / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology
D009279:Naphthacenes; D003609:Dactinomycin; D014212:Tretinoin; D015250:Aclarubicin; D007372:Interferons