[Effects of enzymatic deglycosylation of human goiter thyroglobulin on its immunochemical properties].
Thyroglobulin (Tg), isolated from soluble iodoproteins by ammonium sulphate fractionation, was enzymatically deglycosylated in vitro and analyzed by polyacrylamide gel electrophoresis, double immunodiffusion and non-commercial RIA. Carbohydrate and iodine content was chemically determined. By PAAGE deglycosylated Tg (dTg) showed the appearance of a major band in the 12S region and three slower migrating bands corresponding to higher aggregates than 19S Tg. In immunodiffusion by testing native and deglycosylated Tg against anti-native Tg antiserum it was shown the appearance of a spur of native on deglycosylated Tg. By RIA of native and deglycosylated Tg against anti-deglycosylated Tg antiserum it was shown a minor binding capacity of the anti-deglycosylated antibody against native Tg at high dilutions. The results demonstrate that the enzymatic deglycosylation release almost all the carbohydrates of goiter Tg and that the removal of the carbohydrates of Tg produces a loss of antigenic determinants of the molecule.
D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D005690:Galactose / Q000276:immunology; D005944:Glucosamine / Q000276:immunology; D006026:Glycoside Hydrolases; D006042:Goiter / Q000276:immunology*; D006801:Humans; D005779:Immunodiffusion; D008358:Mannose / Q000276:immunology; D011863:Radioimmunoassay; D012794:Sialic Acids / Q000276:immunology; D013954:Thyroglobulin / Q000276:immunology*
D000939:Epitopes; D012794:Sialic Acids; D013954:Thyroglobulin; D006026:Glycoside Hydrolases; D005944:Glucosamine; D008358:Mannose; D005690:Galactose
Les effets de la déglycosylation enzymatique de la thyroglobuline du goitre humain sur ses propriétés immunochimiques.