[Association of transcription-active DNA fraction with the nuclear skeleton is altered during incubation of nuclei in solutions of low ionic strength].
The experimental conditions ensuring the possibility of isolation of nuclear matrices enriched and non-enriched in transcribed DNA sequences were investigated. It is demonstrated that extraction of nuclease treated nuclei with very low ionic strength solution executed before the high salt extraction destroys the apparent attachment of transcriptionally active DNA to the nuclear skeleton elements. On the contrary RNase treatment of nuclei was found to have no effect on distribution of active genes versus points of DNA attachment to the nuclear skeleton.
D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000032:analysis; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D006825:Hybridomas; D051379:Mice; D009693:Nucleic Acid Hybridization; D009994:Osmolar Concentration; D012313:RNA / Q000302:isolation & purification; D012995:Solubility; D014158:Transcription, Genetic
D012313:RNA; D004247:DNA
Assotsiatsiia transkriptsionno aktivnoĭ fraktsii DNK s iadernym skeletom narushaetsia pri inkubatsii iader v rastvorakh s nizkoĭ ionnoĭ siloĭ.