title: Absorption and metabolism of vitamin K in Swiss 3T3 mouse fibroblasts--a model system for study of vitamin K absorption and metabolism.
abstract: The vitamin K cycle previously described in liver has been demonstrated in Swiss 3T3 mouse fibroblasts. Vitamin K epoxide and gamma-carboxyglutamic acid were isolated from the cells and chemically characterized. Menaquinone (MK4) is also metabolized to its epoxide and vitamin K epoxide is reduced to vitamin K in these cells. Thus Swiss 3T3 mouse fibroblasts provide a useful model system for the study of vitamin K metabolism. Possible functions of the vitamin K-dependent protein(s) in fibroblasts are discussed.
mesh_terms: D015055:1-Carboxyglutamic Acid / Q000378:metabolism; D000042:Absorption; D000818:Animals; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D001728:Dicumarol / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D013058:Mass Spectrometry; D051379:Mice; D008954:Models, Biological; D014812:Vitamin K / Q000378:metabolism*; D010837:Vitamin K 1 / Q000031:analogs & derivatives / Q000378:metabolism; D014859:Warfarin / Q000494:pharmacology
chemical_list: D014812:Vitamin K; C016186:vitamin K1 oxide; D015055:1-Carboxyglutamic Acid; D014859:Warfarin; D001728:Dicumarol; D010837:Vitamin K 1