RNA binding fragments from nucleolin contain the ribonucleoprotein consensus sequence.
Nucleolin (C23 or 100 kDa) is a major nucleolar phosphoprotein whose primary structure has recently been determined (Lapeyre, B., Bourbon, H., and Amalric, F. (1987) Proc. Natl. Acad. Sci. U. S. A. 84, 1472-1476) and found to be associated with preribosomal RNA (Herrera, A. H., and Olson, M. O. J. (1986) Biochemistry 25, 6258-6263). To identify the RNA binding region of the molecule, cyanogen bromide fragments were tested for binding of 18 S and 28 S ribosomal RNA by a "Western blotting" technique. Fragments with apparent molecular masses of 13, 33, and 47 kDa bound RNA with no preference for either 18 S or 28 S RNA. By protein sequencing, these fragments were localized in the carboxyl-terminal two-thirds of the molecule. The nucleolin sequence was searched for the ribonucleoprotein consensus sequence found in other RNA binding proteins. Four copies of a closely related 11-residue sequence were found within 80-90 residue repeats in the RNA binding region between residues 285 and 629. These results suggest that a highly conserved structure for the binding of different classes of RNA is utilized by several proteins.
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003488:Cyanogen Bromide; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D009698:Nucleoproteins / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D011061:Poly A / Q000378:metabolism; D012313:RNA / Q000378:metabolism*; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; D000097796:Nucleolin
D003488:Cyanogen Bromide; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D009698:Nucleoproteins; D010446:Peptide Fragments; D010750:Phosphoproteins; D011061:Poly A; D012313:RNA; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; D000097796:Nucleolin