Fibroblast growth factor stabilizes ribonucleic acid and regulates differentiated functions in a multipeptide-secreting neuroendocrine cell line.
A clonal cell line (44-2C) which synthesizes and secretes somatostatin, neurotensin, calcitonin (CT), and CT gene-related peptide and transiently expresses c-fos was used to characterize the mechanism of action of basic fibroblast growth factor (bFGF). bFGF had two modes of action: 1) short term incubation of 44-2C cells with bFGF increased the cellular content of neurotensin, somatostatin, and CT; and 2) bFGF enhanced the response of the cells to rat hypothalamic GRF-mediated cAMP efflux. The long term action of bFGF was manifested by the permissive effect of the molecule. bFGF had a sustained effect on RNA synthesis, and pretreatment with bFGF for 24 h altered the time course of response of the cells to rat GRF. In this cell line the cellular action of bFGF was not mediated via protein kinase-C action. bFGF was not mitogenic in 44-2C cells. bFGF stimulated uridine incorporation without affecting thymidine incorporation. Results obtained with actinomycin-D and alpha-amanitin suggest that the above effects of bFGF can be correlated with increased RNA stability produced by bFGF.
D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D002116:Calcitonin / Q000378:metabolism; D002460:Cell Line; D002999:Clone Cells; D000242:Cyclic AMP / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D013007:Growth Hormone-Releasing Hormone / Q000494:pharmacology; D007700:Kinetics; D009490:Neurosecretory Systems / Q000378:metabolism*; D009496:Neurotensin / Q000378:metabolism; D015240:Phorbol 12,13-Dibutyrate; D010703:Phorbol Esters / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis*; D051381:Rats; D013004:Somatostatin / Q000378:metabolism
D000546:Amanitins; D010703:Phorbol Esters; D003609:Dactinomycin; D015240:Phorbol 12,13-Dibutyrate; D009496:Neurotensin; D013004:Somatostatin; D005346:Fibroblast Growth Factors; D012313:RNA; D002116:Calcitonin; D013007:Growth Hormone-Releasing Hormone; D000242:Cyclic AMP; D011493:Protein Kinase C