Bradykinin inhibits potassium (M) currents in N1E-115 neuroblastoma cells. Responses resemble those in NG108-15 neuroblastoma x glioma hybrid cells.
Application of bradykinin to voltage-clamped N1E-115 mouse neuroblastoma cells evoked sequential outward and inward membrane currents, accompanied by an increase and decrease of membrane conductance, respectively. Methacholine produced an inward current with a decreased conductance. The outward current response to bradykinin was imitated by intracellular inositol 1,4,5-trisphosphate (IP3). Bath application of phorbol dibutyrate induced an inward current and potentiated the response to IP3. We conclude that the response of these cells to bradykinin is identical to that of NG108-15 hybrid cells, and therefore may be attributed to the dual effects of inositol trisphosphate and diacylglycerol formed by hydrolysis of phosphatidylinositide.
D000818:Animals; D001920:Bradykinin / Q000494:pharmacology*; D002460:Cell Line; D004553:Electric Conductivity; D005910:Glioma / Q000503:physiopathology; D006822:Hybrid Cells; D015544:Inositol 1,4,5-Trisphosphate; D007295:Inositol Phosphates / Q000494:pharmacology; D007473:Ion Channels / Q000187:drug effects*; D008564:Membrane Potentials; D051379:Mice; D009447:Neuroblastoma / Q000503:physiopathology*; D010703:Phorbol Esters / Q000494:pharmacology; D011188:Potassium / Q000037:antagonists & inhibitors*
D007295:Inositol Phosphates; D007473:Ion Channels; D010703:Phorbol Esters; D015544:Inositol 1,4,5-Trisphosphate; D011188:Potassium; D001920:Bradykinin