Murine monoclonal antibodies to human A erythrocytes: differential reactivity with N-acetyl-D-galactosamine.
Six different hybridoma cell lines were obtained producing IgM monoclonal antibodies (MAbs) against human blood group A antigen. All these MAbs agglutinate the A1, A1B and A2 erythrocytes, while only one of them agglutinates the A2B erythrocytes. The agglutination activity of 3 of these MAbs is inhibited by N-acetyl-D-galactosamine (D-GalNAc). Dissociation constants of complexes of these MAbs with D-GalNAc are approximately 2.2 X 10(-3) mol/l.
D000017:ABO Blood-Group System / Q000276:immunology*; D000116:Acetylgalactosamine / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000939:Epitopes / Q000276:immunology; D005688:Galactosamine / Q000031:analogs & derivatives*; D006386:Hemagglutination Tests; D006801:Humans; D051379:Mice
D000017:ABO Blood-Group System; D000911:Antibodies, Monoclonal; D000939:Epitopes; D005688:Galactosamine; D000116:Acetylgalactosamine