Investigation into the use of graft copolymer-based cibacron blue F3GA columns for the purification of proteins.
The graft copolymers Nylon-co-hydroxyethylmethacrylate and poly(ethylene)-co-hydroxyethylmethacrylate coupled to Cibacron blue F3GA at wet volume levels similar to those obtained with Sepharose 4B. However, the graft copolymers removed protein from human serum to a far lesser extent than did Sepharose 4B. Further investigations involved the preparation of hydrolyzed poly(vinyl acetate) copolymers of nylon and polyethylene and of cellulose-co-hydroxyethylmethacrylate and study of the ability of the copolymers to remove human serum albumin and lactic dehydrogenase. Comparisons were made with Sepharose 4B-, Sephadex G15-, and G25-based Cibacron blue F3GA systems. The effectiveness of Sepharose 4B-Cibacron blue F3GA is thought to be due to the manner in which the dye is located within the pores of the gel.
D000327:Adsorption; D001798:Blood Proteins / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D003911:Dextrans; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000302:isolation & purification; D009757:Nylons; D011095:Polyethylenes; D012685:Sepharose; D012709:Serum Albumin / Q000302:isolation & purification; D014227:Triazines
D001798:Blood Proteins; D003911:Dextrans; D009757:Nylons; D011095:Polyethylenes; D012709:Serum Albumin; D014227:Triazines; C012842:Cibacron Blue F 3GA; D012685:Sepharose; C025614:sephadex; D007770:L-Lactate Dehydrogenase