title: The identification of inhibitors in bromelin one-stage irregular antibody screening techniques.
abstract: Human sera contain bromelin inhibitors in irregular antibody screening techniques. In order to identify and purify these inhibitors, pooled human sera were precipitated with ammonium sulfate, followed by DEAE-cellulose and Sephadex G-150 column chromatography. Proteinase activity and inhibitory activity to bromelin were observed in two protein fractions, these were identified as alpha 2-macroglobulin and the S-2 thiol proteinase inhibitor.
mesh_terms: D000906:Antibodies / Q000032:analysis*; D001963:Bromelains / Q000037:antagonists & inhibitors*; D003546:Cysteine Endopeptidases; D010450:Endopeptidases; D004791:Enzyme Inhibitors / Q000097:blood*; D006386:Hemagglutination Tests; D006801:Humans; D008722:Methods; D011480:Protease Inhibitors; D000511:alpha-Macroglobulins / Q000032:analysis
chemical_list: D000906:Antibodies; D004791:Enzyme Inhibitors; D011480:Protease Inhibitors; D000511:alpha-Macroglobulins; D001963:Bromelains; D010450:Endopeptidases; D003546:Cysteine Endopeptidases