title: The in vitro effects of cyclic nucleotides, cyanoketone, and cycloheximide on the production of estradiol-17 beta by vitellogenic ovarian follicles of goldfish (Carassius auratus).
abstract: The effects of human chorionic gonadotropin (HCG), forskolin, cyclic nucleotides, the phosphodiesterase inhibitors IBMX and theophylline, cyanoketone, and cycloheximide on the production of estradiol-17 beta by isolated ovarian follicles of vitellogenic goldfish (Carassius auratus) were examined using 18-hr incubations. HCG and all test agents which are known to increase intracellular concentrations of cAMP significantly stimulated the production of estradiol-17 beta. However, dibutyryl cGMP was unable to stimulate estradiol-17 beta production at any concentration used (1-10 mM). Cyanoketone at a concentration of 1 micrograms/ml completely blocked forskolin-induced estradiol-17 beta production. Even in the presence of cyanoketone, however, forskolin stimulated conversion of exogenous testosterone to estradiol-17 beta in a dose-dependent manner, suggesting the involvement of an adenylate-cyclase system in the induction of aromatase activation by vitellogenic follicles of goldfish. Cycloheximide also completely abolished HCG-induced estradiol-17 beta production when this inhibitor was added within the first 1 hr after the addition of HCG. These results provide evidence that the stimulation of estradiol-17 beta by goldfish vitellogenic follicles in response to HCG is dependent upon the synthesis of new protein.
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000737:Androstenols / Q000494:pharmacology*; D000818:Animals; D001141:Aromatase / Q000378:metabolism; D003994:Bucladesine / Q000494:pharmacology; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D003489:Cyanoketone / Q000494:pharmacology*; D000242:Cyclic AMP / Q000494:pharmacology; D006152:Cyclic GMP / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology*; D003530:Cyprinidae / Q000378:metabolism*; D003995:Dibutyryl Cyclic GMP / Q000494:pharmacology; D004958:Estradiol / Q000096:biosynthesis*; D005260:Female; D006054:Goldfish / Q000378:metabolism*; D009712:Nucleotides, Cyclic / Q000494:pharmacology*; D006080:Ovarian Follicle / Q000378:metabolism*; D013806:Theophylline / Q000494:pharmacology; D013997:Time Factors; D014818:Vitellogenesis
chemical_list: D000737:Androstenols; D006063:Chorionic Gonadotropin; D009712:Nucleotides, Cyclic; D003995:Dibutyryl Cyclic GMP; D003489:Cyanoketone; D004958:Estradiol; D003994:Bucladesine; D003513:Cycloheximide; D013806:Theophylline; D000242:Cyclic AMP; D001141:Aromatase; D006152:Cyclic GMP; D015056:1-Methyl-3-isobutylxanthine