title: Oligomycin inhibition of Na,K,ATPase. Analysis of half-of-sites moderator interaction with a dimeric enzyme.
abstract: It is shown that the incomplete, uncompetitive inhibition pattern exhibited by oligomycin toward Na,K,ATPase cannot be explained by a single-cycle enzyme model. In contrast, the experimental data are easily explained in terms of a dimeric enzyme, only one subunit of which can bind oligomycin at a time, and that subunit is then rendered inactive. In a brief analysis of the model thus obtained by way of numerical examples it is shown that it may show activation at small concentrations of moderator, which disappears at higher concentrations, a property observed for the hydrolysis of p-nitro-phenylphosphate, which is also catalyzed by Na,K,ATPase.
mesh_terms: D000818:Animals; D046911:Macromolecular Substances; D008433:Mathematics; D008962:Models, Theoretical; D009840:Oligomycins / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*; D013329:Structure-Activity Relationship
chemical_list: D046911:Macromolecular Substances; D009840:Oligomycins; D000254:Sodium-Potassium-Exchanging ATPase