A study of the mechanism of ocular irritation following YAG laser capsulotomy in rabbits.
The irritative response to Nd:YAG laser capsulotomy was studied in unanaesthetized rabbits. Posterior lens capsulotomy with a total energy of 100 mJ had no effect on the pupil size but increased the intraocular pressure by 5-10 mmHg and caused a breakdown of the blood-aqueous barrier. Anterior lens capsulotomy with a total energy of 20, 60 or 100 mJ caused constriction of the pupil, and an increase in intraocular pressure in a dose-dependent manner, and a breakdown of the blood-aqueous barrier. Indomethacin attenuated all the component parts of the irritative response and (D-arg1, D-pro2, D-trp7,9, leu11)-SP attenuated the miotic response. A combination of indomethacin and the substance P antagonist almost completely abolished the irritative response. This indicates that the acute YAG-laser-induced irritation in the rabbit eye is dependent both on a release of prostaglandins and on substance P, the former probably releasing the latter from sensory nerves.
D000818:Animals; D001082:Aqueous Humor / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005128:Eye Diseases / Q000209:etiology* / Q000517:prevention & control; D005136:Eye Proteins / Q000032:analysis; D005260:Female; D007213:Indomethacin / Q000627:therapeutic use; D007429:Intraocular Pressure / Q000187:drug effects; D053685:Laser Therapy / Q000009:adverse effects*; D007903:Lens Capsule, Crystalline / Q000601:surgery*; D007908:Lens, Crystalline / Q000601:surgery*; D008297:Male; D011680:Pupil; D011817:Rabbits; D011994:Recombinant Proteins; D013373:Substance P / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000494:pharmacology
D005136:Eye Proteins; D011994:Recombinant Proteins; D013373:Substance P; C050702:substance P, Arg(1)-Pro(2)-Trp(7,9)-Leu(11)-; D007213:Indomethacin