Transfection and amplification of the chicken c-src proto-oncogene in Rat-1 cells.
The chicken cellular proto-oncogene c-src was cotransfected into normal Rat-1 cells with the mouse dhfr gene. Selection for amplification of dhfr sequences resulted in co-amplification of the chicken c-src gene. Cell clones expressing varying levels of c-src associated kinase activity were isolated, none of these had a transformed morphology. In contrast, expression of v-src in Rat-1 cells resulted in morphological transformation and the ability to grow in soft agar in an anchorage independent way.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D002645:Chickens; D005784:Gene Amplification; D005786:Gene Expression Regulation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016392:Proto-Oncogene Proteins pp60(c-src); D011519:Proto-Oncogenes; D051381:Rats; D014162:Transfection
D011518:Proto-Oncogene Proteins; D011505:Protein-Tyrosine Kinases; D016392:Proto-Oncogene Proteins pp60(c-src)