title: [The effect of the cell separation procedure on neutrophilic granulocyte function. II. Research on the bactericidal capacity and the NBT reduction ability of isolated granulocytes].
abstract: The investigations were aimed at examining the impact of methodically simple separating and enriching techniques for gaining granulocytes on the bactericidal capacity and NBT reduction in isolated granulocytes. For this purpose, granulocytes were gained by the technique of sedimentation and flotation. Granulocytes isolated by the cell separator Haemonetics model 30 were used as reference material. The findings of the investigations together with those of previous studies revealed no indication of the granulocyte function and morphology being damaged by the separating techniques used.
mesh_terms: D001770:Blood Bactericidal Activity; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods; D004926:Escherichia coli; D006098:Granulocytes / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D009580:Nitroblue Tetrazolium / Q000097:blood*; D010084:Oxidation-Reduction; D013778:Tetrazolium Salts / Q000097:blood*; D013997:Time Factors
chemical_list: D013778:Tetrazolium Salts; D009580:Nitroblue Tetrazolium
vernacular_title: Der Einfluss von Zelltrennverfahren auf die Funktion der neutrophilen Granulozyten. II. Untersuchungen zur Bakterizidiekapazität und zum NBT-Reduktionsvermögen von isolierten Granulozyten.