Use of gold IgG complexes and human antisera for electron microscopic identification of hepatitis A virus and polioviruses.
An indirect immunogold staining technique was used for identification of hepatitis A virus and poliovirus types 1,2 and 3 by electron microscopy. Human convalescent antisera against hepatitis A virus and the three poliovirus types were used as primary antibodies and goat anti-human IgG gold complexes, with an approximate gold granule size of 15 nm, as secondary antibody. Hepatitis A virus was specifically labelled with the homologous antiserum. No labelling was observed when antiserum against poliovirus or other negative control sera were used as primary antibody. Poliovirus types 1, 2 and 3 were specifically labelled with a human serum with high titres of antibody against all three virus types. The use of type-specific antisera made it possible to distinguish types 1, 2 and 3 from each other, although slight cross-reactions occurred. The results indicate that gold IgG complexes in conjunction with human antisera may be used for identification of small spherical viruses.
D000914:Antibodies, Viral / Q000032:analysis; D003102:Colloids; D003429:Cross Reactions; D006046:Gold; D006507:Hepatovirus / Q000302:isolation & purification*; D006801:Humans; D007106:Immune Sera; D007074:Immunoglobulin G; D007158:Immunologic Techniques; D008854:Microscopy, Electron; D017955:Poliovirus / Q000302:isolation & purification*; D013194:Staining and Labeling
D000914:Antibodies, Viral; D003102:Colloids; D007106:Immune Sera; D007074:Immunoglobulin G; D006046:Gold