Characterization and incompatibility properties of ROM- derivatives of pBR322-based plasmids.
We have characterized a copy number mutant of the pBR322-based plasmid pWT111. A single nucleotide transversion in loop II' of RNAI results in an eightfold increase in plasmid copy number. Removal of the rom coding region from pWT111 cop results in a further sixfold increase in copy number. We present evidence that ROM is involved in the strong incompatibility effect seen between pMB1 and ColE1 type plasmids.
D001483:Base Sequence; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000235:genetics*; D009154:Mutation; D009690:Nucleic Acid Conformation; D010957:Plasmids; D012329:RNA, Bacterial
D004269:DNA, Bacterial; D012329:RNA, Bacterial