[Structural-functional characteristics of argiopine--the ion channel blockers from the spider Argiope lobata venom].
Argiopine, a compound capable of blocking the glutamate-activated ion channels in experiments with glutamatergic synapses of blowfly larvae, was isolated from the venom of spider Argiope lobata. Argiopine-receptor complex has KD 6,7 X 10(-7) M. The venom solution was treated with 60% ethanol, centrifuged and supernatant was subjected to reversed-phase HPLC in the acetonitrile concentration gradient. Argiopine structure was established using 1H- and 13C-NMR spectroscopy, mass spectrometry, elemental and amino acid analyses. Argiopine (molecular mass 636) consists of arginine (free alpha-NH2) linke with polyamine--NH (CH2)3NH(CH2)3NH(CH2)5NH--through a peptide bond. The polyamine is connected to the alpha-carboxyl group of asparagine whose alpha-amino group is linked to 2,4-dihydroxyphenylacetic acid.
D000818:Animals; D001180:Arthropod Venoms / Q000494:pharmacology*; D055598:Chemical Phenomena; D002621:Chemistry; D004175:Diptera; D005971:Glutamates / Q000378:metabolism*; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects*; D008970:Molecular Weight; D009842:Oligopeptides / Q000032:analysis / Q000494:pharmacology*; D011073:Polyamines / Q000032:analysis / Q000494:pharmacology*; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter / Q000187:drug effects*; D013111:Spider Venoms / Q000032:analysis / Q000494:pharmacology*
D001180:Arthropod Venoms; D005971:Glutamates; D007473:Ion Channels; D009842:Oligopeptides; D011073:Polyamines; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter; D013111:Spider Venoms; C050570:argiopine
Strukturno-funktsional'naia kharakteristika argiopina--blokatora ionnykh kanalov iz iada pauka Argiope lobata.