A flow cytometric procedure for the isolation of antigenically responsive human basophils.
A flow cytometric procedure is described for the isolation of human basophils. The basophils are isolated by cell sorting after passive sensitization with mouse FITC-IgE anti-DNP, and the isolated basophils exhibit characteristic histamine release in response to incubation with DNP21-human serum albumin conjugates. Using this method, 1-4 X 10(5) basophils can be isolated at greater than or equal to 98% purity from approximately 30 ml whole blood in a 2 h sorting procedure.
D001491:Basophils / Q000166:cytology* / Q000276:immunology; D002469:Cell Separation / Q000379:methods; D005434:Flow Cytometry; D006636:Histamine Release; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007073:Immunoglobulin E / Q000276:immunology; D011961:Receptors, Fc / Q000378:metabolism
D011961:Receptors, Fc; D007073:Immunoglobulin E