Relationship between T200 antigen expression and stages of B cell differentiation in resurgent hyperplasia of bone marrow.
Using monoclonal antibodies (MoAbs) and dual-parameter flow cytometric techniques, bone marrow mononuclear cells (MMC) from patients with resurgent hyperplasia were analyzed for their coexpression of HLe-1 (T200) and antigens normally associated with particular stages of B cell differentiation. The marrow from those with resurgent hyperplasia contained increased numbers of B cell precursors in multiple stages of differentiation compared to controls, thus providing a useful model system for studies of B cell differentiation. These studies indicate that the quantitative expression of T200 is differentiation-related on normal and malignant B cells and B cell precursors. Immature cells express low amounts of T200, while increasing levels of maturity correlated with increasing amounts of the antigen. This study increases the understanding of relationships between B cell surface antigens and T200 and further demonstrates that B cell hyperplasia occurs commonly in association with bone marrow reactive or resurgent processes. The quantitative, rather than only the qualitative, expression of T200 is therefore a useful marker of B cell differentiation in reactive hyperplasia and in further investigation of B cell malignancy.
D000293:Adolescent; D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology*; D001853:Bone Marrow / Q000473:pathology*; D002454:Cell Differentiation; D002648:Child; D002675:Child, Preschool; D005453:Fluorescence; D006649:Histocompatibility Antigens / Q000276:immunology*; D006801:Humans; D006965:Hyperplasia / Q000276:immunology / Q000473:pathology; D017493:Leukocyte Common Antigens; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D006649:Histocompatibility Antigens; D017493:Leukocyte Common Antigens