A new family of LTR-like sequences abundantly expressed in rat tumors.
We report the identification of two genome DNA fragments containing middle repetitive sequences abundantly expressed in various rat tumors but rarely in normal tissues. These fragments included homologous regions which belonged to a new family of long terminal repeat (LTR) like sequences, designated RAL elements; one displayed the solitary type and the other a provirus structure. The element was transcribed in a strand specific fashion and started from the presumptive cap site within the RAL element. The presumptive polyA addition site within the element was also utilized as evidence of the analysis of a cDNA clone containing the RAL element. This evidence suggested that transcriptional control signals within the element were functioning. Run on assay revealed that expression of the element was regulated at the transcriptional level.
D000818:Animals; D004247:DNA / Q000032:analysis; D004273:DNA, Neoplasm / Q000032:analysis*; D005786:Gene Expression Regulation; D009374:Neoplasms, Experimental / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000032:analysis; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger; D051381:Rats; D011919:Rats, Inbred Strains; D012091:Repetitive Sequences, Nucleic Acid; D012190:Retroviridae / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D004273:DNA, Neoplasm; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA