Reversible osmotic opening of the blood-brain barrier in mice.
Unilateral reversible osmotic opening of the blood-brain barrier can be produced in mice. Infusion of 1.8 molal arabinose in water at a rate of 0.64 ml/min for 30 seconds into the internal carotid artery consistently results in ipsilateral brain staining by intravascular Evans blue dye. Osmotic opening is concentration-dependent (threshold, 1.6 molal arabinose) and reversible within 4 hours. No long-term neurologic deficit occurs. These results suggest that reversible osmotic blood-brain barrier opening can be applied to disease models in mice.
D000818:Animals; D001089:Arabinose / Q000493:pharmacokinetics*; D001812:Blood-Brain Barrier / Q000187:drug effects*; D001921:Brain / Q000033:anatomy & histology; D005070:Evans Blue; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D009995:Osmosis / Q000187:drug effects; D013194:Staining and Labeling; D013997:Time Factors
D005070:Evans Blue; D001089:Arabinose