Analysis of human antibody response to cholera vaccination by Epstein Barr virus transformation and human-human hybridoma production.
The response of Epstein Barr virus transformable B lymphocytes to V. cholerae serotypes Inaba and Ogawa was assessed at various periods of time after primary and secondary immunization. In both cases, 100% of cells laid down at 10(4)/well gave a very strong positive reaction with either serotype one week after immunization. Further analysis indicated that general polyclonal activation by the bacterial LPS was not a major feature since the reactivity with other bacterial antigens was weak. Human monoclonal antibodies generated from the primary wells by backfusion with the KR4 cell line had low affinity in comparison to rat monoclonal antibodies reactive with the same antigen but were serotype specific and able to fix guinea pig complement.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007117:Immunization, Secondary; D007118:Immunoassay / Q000379:methods; D008213:Lymphocyte Activation; D051381:Rats; D013997:Time Factors; D014611:Vaccination; D014734:Vibrio cholerae / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes