Inactive X chromosome gene reactivation induced by 5-azacytidine, is not correlated with the modification of the pattern of replication.
The replication pattern of a human inactive X chromosome reactivated for one to four genes by 5-azacytidine has been studied by the BrdU-33258-Hoechst-Giemsa technique in four subclones of a somatic hamster-human hybrid. In one of them the pattern was clearly modified. In the three others the changes were not significant. No correlation was found between expression and replication.
D000818:Animals; D001374:Azacitidine / Q000494:pharmacology*; D002460:Cell Line; D006224:Cricetinae; D004261:DNA Replication; D005786:Gene Expression Regulation; D006801:Humans; D006822:Hybrid Cells / Q000187:drug effects*; D014960:X Chromosome / Q000187:drug effects*
D001374:Azacitidine