The effects of trifluoperazine on fast and slow axonal transport in the rabbit vagus nerve.
The effects of trifluoperazine (TFP) on fast and slow axonal transport (AXT) of labeled proteins were examined in the rabbit vagus nerve. Cuffs soaked in a 10 mM, but not 0.1 mM or 1 mM, concentration of TFP applied locally around the vagus nerve in vivo blocked both fast and slow AXT, as measured by the accumulation of 3H-labeled proteins. In vitro, fast AXT was affected by 0.1 mM TFP. The TFP cuff treatment caused a reduction in the number of axonal microtubules (MT) whereas cuffs soaked in saline had no effect. The levels of ATP, ADP, and AMP were not significantly lowered by the TFP treatment. The results suggest that both fast and slow AXT are sensitive to TFP treatment, and that the axonal MT-system may be the main target of the drug.
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D001370:Axonal Transport / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D008854:Microscopy, Electron; D011506:Proteins / Q000378:metabolism; D011817:Rabbits; D013997:Time Factors; D014268:Trifluoperazine / Q000494:pharmacology*; D014630:Vagus Nerve / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure
D000227:Adenine Nucleotides; D011506:Proteins; D014268:Trifluoperazine