Ultrastructural, immunocytochemical, and cytogenetic characterization of a human epithelioid sarcoma cell line (RM-HS1).
A cell line (RM-HS1) derived from a human epithelioid sarcoma was established in tissue culture. Ultrastructurally, the cells show features of those found within the primary tumor. A mixed mesenchymal-epithelial phenotype, defined by reactivity with antibodies to epithelial membrane antigen and to vimentin and keratin intermediate filaments, was found in the tumor, and a similar phenotype persisted in the cultured cells. Cytogenetic analysis revealed a mode of 66 chromosomes. With the use of a variety of banding techniques together with in situ hybridization of a 3H-labeled molecular probe for 18s and 28s ribosomal RNA genes (pX1r101), the karyotypes were shown to contain extensive numerical and structural rearrangements, with up to 24 marker chromosomes.
D000328:Adult; D000782:Aneuploidy; D000951:Antigens, Neoplasm / Q000032:analysis; D002460:Cell Line; D002869:Chromosome Aberrations; D066246:ErbB Receptors / Q000032:analysis; D005534:Foot Diseases / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007633:Keratins / Q000032:analysis; D008297:Male; D008565:Membrane Proteins / Q000032:analysis; D018396:Mucin-1; D009363:Neoplasm Proteins / Q000032:analysis; D012509:Sarcoma / Q000032:analysis / Q000235:genetics / Q000648:ultrastructure*; D014746:Vimentin / Q000032:analysis
D000951:Antigens, Neoplasm; D008565:Membrane Proteins; D018396:Mucin-1; D009363:Neoplasm Proteins; D014746:Vimentin; D007633:Keratins; D066246:ErbB Receptors