Topography of N-CAM structural and functional determinants. II. Placement of monoclonal antibody epitopes.
The accompanying report (Watanabe, M., A. L. Frelinger III, and U. Rutishauser, 1986, J. Cell Biol., 103:1721-1727) describes a set of monoclonal antibodies (mAbs) directed against N-CAM epitopes representing the known major structural and functional domains of the molecule. In this study, we have generated and separated a variety of peptide fragments from N-CAM, and then used their size and reactivity with each antibody to position the antigenic sites along the peptide chain. This epitope map, together with the biological properties of the antibodies and previous studies on N-CAM, have been used to construct a topographical model for the molecule in the cell membrane.
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D001665:Binding Sites; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules; D002642:Chick Embryo; D003593:Cytoplasm / Q000276:immunology; D000939:Epitopes; D005110:Extracellular Space / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology*; D008565:Membrane Proteins / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology; D011487:Protein Conformation
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D000939:Epitopes; D006023:Glycoproteins; D008565:Membrane Proteins; D010446:Peptide Fragments