Mutagenicity of diesel exhaust particles and oil shale particles dispersed in lecithin surfactant.
Diesel exhaust particulate material from exhaust pipe scrapings of two trucks, diluted automobile diesel exhaust particulate material collected on filters, and two oil shale ores were prepared for the Ames mutagenicity assay by dichloromethane (DCM) extraction, by dispersion into 0.85% saline, or by dispersion into dipalmitoyl lecithin (DPL) emulsion in saline. Salmonella typhimurium TA98 was used to detect frameshift mutagens in the samples. Samples of diesel soot gave positive mutagenic responses with both DCM extraction and DPL dispersion, with the DPL dispersion giving higher results in some cases. The results suggest that possible mutagens associated with inhaled particles may be dispersed or solubilized into the phospholipid component of pulmonary surfactant and become active in such a phase.
D004134:Dimyristoylphosphatidylcholine; D005648:Fuel Oils; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology*; D009154:Mutation; D010578:Petroleum; D012486:Salmonella typhimurium / Q000187:drug effects; D012822:Silicon Dioxide / Q000494:pharmacology*; D001335:Vehicle Emissions
D005648:Fuel Oils; D009153:Mutagens; D010578:Petroleum; D001335:Vehicle Emissions; D012822:Silicon Dioxide; D004134:Dimyristoylphosphatidylcholine