Demonstration of Epstein-Barr virus in malignant non-Hodgkin's lymphomas.
Lymph nodes or tumor biopsies of 60 persons suspected of having a malignant lymphoma were examined for the presence of Epstein-Barr virus nuclear antigen (EBNA) by anticomplement immunofluorescence. In 8 cases the tissue specimens were also assayed for Epstein-Barr virus (EBV) DNA by nucleic acid hybridization. Serum samples of patients and controls were tested for EBV-related antibodies. The histological tests in 37 cases showed a malignant non-Hodgkin lymphoma, and in 23 cases a reactive lymphadenopathy. A Burkitt lymphoma of a European boy and a polymorphic centroblastoma contained EBNA and approximately 27 or 30 genome equivalents EBV DNA per cell, respectively. EBNA was also demonstrated in about 20% of the cells of a lymph node from a patient with recurrent reactive lymphadenopathy.
D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D002051:Burkitt Lymphoma / Q000382:microbiology; D002648:Child; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005455:Fluorescent Antibody Technique; D006258:Head and Neck Neoplasms / Q000382:microbiology; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology*; D006651:Histocytochemistry; D006801:Humans; D008198:Lymph Nodes / Q000378:metabolism; D008223:Lymphoma / Q000382:microbiology* / Q000401:mortality; D008297:Male; D008875:Middle Aged; D013194:Staining and Labeling; D014412:Tumor Virus Infections
D000914:Antibodies, Viral; D000956:Antigens, Viral; D004279:DNA, Viral