Detection and differentiation by sandwich enzyme-linked immunosorbent assay of human T-cell lymphotropic virus type III/lymphadenopathy-associated virus- and acquired immunodeficiency syndrome-associated retroviruslike clinical isolates.
Monoclonal antibodies can be used in sandwich enzyme-linked immunosorbent assays to measure viral antigens. Such an assay was developed to detect the core protein, p24, of human T-cell lymphotropic virus type III and lymphadenopathy-associated virus, etiologic agents of the acquired immunodeficiency syndrome (AIDS). Another AIDS-associated virus, AIDS-associated retrovirus type 2 (ARV-2) could not be detected in this assay because of the low affinity of one of the monoclonal antibodies to ARV-2 p24. Detection of ARV-2 was accomplished with a monoclonal antibody-rabbit polyclonal antibody sandwich enzyme-linked immunosorbent assay. These two assays were used to efficiently detect AIDS-related viruses in lymphocyte cell cultures and to distinguish strains of the viruses.
D000386:AIDS-Related Complex / Q000382:microbiology; D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D006678:HIV / Q000276:immunology / Q000302:isolation & purification*; D015488:HIV Antigens; D006801:Humans; D008214:Lymphocytes / Q000276:immunology / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D012367:RNA, Viral / Q000032:analysis; D011817:Rabbits; D014758:Viral Core Proteins / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes; D015488:HIV Antigens; D012367:RNA, Viral; D014758:Viral Core Proteins