The positive chronotropic effects of Bay K-8644 and calcium as influenced by temperature.
The positive chronotropic effects of the dihydropyridine calcium agonist BAY K-8644 and calcium were studied in spontaneously beating right guinea-pig atria. Within the temperature range from 22 to 37 degrees C, the following observations were made: (1) The basal pacemaker frequency increased linearly with the ambient temperature; (2) the increase in pacemaker rate produced by a maximally effective concentration of BAY K-8644 was of the same magnitude as that produced by calcium; (3) the increases in pacemaker rate produced by BAY K-8644 and by calcium were directly proportional to the increase in temperature above 22 degrees C; and (4) at 37 degrees C, the apparent ED50 for the positive chronotropic effect of calcium was 2.3 X 10(-3) M and of BAY K-8644 was 0.7 X 10(-7) M. At temperatures below 22 degrees C, irregular pacemaking activity or complete arrest of pacemaking activity frequently occurred. The data are in strong support of the assumption that BAY K-8644 elicits its positive chronotropic effect by increasing the availability of calcium ions.
D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology*; D006168:Guinea Pigs; D006325:Heart Atria; D006339:Heart Rate / Q000187:drug effects*; D066298:In Vitro Techniques; D009206:Myocardium / Q000378:metabolism; D013268:Stimulation, Chemical; D013696:Temperature
D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; D002118:Calcium