Transformation of Aspergillus nidulans by the argB gene.
Aspergillus nidulans argB mutant was transformed with the plasmid DNA containing the argB gene. Analysis of transformants revealed that transformation was due to integration of either argB gene alone or the whole plasmid DNA into the A. nidulans genome. In 5 out of 23 transformants studied, integration took place in the locus different than the original argB locus. The amplification of integrated sequences was often observed. Integrated DNA was found to be mitotically stable, while the meiotic stability depends on the mode of integration. The activity of the ornithine carbamoyltransferase (the argB gene product) was measured and in some transformants bearing the amplified argB sequence was found to be strongly elevated.
D001233:Aspergillus nidulans / Q000201:enzymology / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004271:DNA, Fungal / Q000032:analysis*; D005800:Genes, Fungal; D005822:Genetic Vectors; D008745:Methylation; D009693:Nucleic Acid Hybridization; D009954:Ornithine Carbamoyltransferase / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D014170:Transformation, Genetic
D004271:DNA, Fungal; D009954:Ornithine Carbamoyltransferase; D004262:DNA Restriction Enzymes