Adsorption of plasma proteins and platelet adhesion on to polydimethylsiloxane/poly(gamma-benzyl L-glutamate) block copolymer films.
A-B-A-type block copolymers of four different compositions were synthesized, in which A and B represent poly(gamma-benzyl L-glutamate) and polydimethylsiloxane segments, respectively. Among the block copolymers and their surface-modified derivatives, those containing 40-70 mol% polydimethylsiloxane and having water contact angles ranging from 50 degrees to 85 degrees were found not to induce conformational change of plasma proteins upon adsorption. It was also observed that the number of adhered platelets and the rate of serotonin release from adhered platelets increased when plasma proteins underwent conformational change upon adsorption. These experimental observations indicate that hydrophobic-hydrophilic block copolymers having a certain composition do not induce conformational change of plasma proteins upon adsorption and do not adhere to and activate platelets, thus leading to a suppression of thrombus formation.
D000327:Adsorption; D001672:Biocompatible Materials; D001792:Blood Platelets / Q000502:physiology; D001798:Blood Proteins; D004129:Dimethylpolysiloxanes; D005340:Fibrinogen; D006801:Humans; D008854:Microscopy, Electron; D010455:Peptides; D010973:Platelet Adhesiveness; D011099:Polyglutamic Acid / Q000031:analogs & derivatives; D011487:Protein Conformation; D012701:Serotonin / Q000097:blood; D012710:Serum Albumin, Bovine; D012828:Silicones; D013055:Spectrophotometry, Infrared; D013499:Surface Properties; D005719:gamma-Globulins
D001672:Biocompatible Materials; D001798:Blood Proteins; D004129:Dimethylpolysiloxanes; D010455:Peptides; D012828:Silicones; D005719:gamma-Globulins; C003172:poly-gamma-benzyl-L-glutamate; D011099:Polyglutamic Acid; D012710:Serum Albumin, Bovine; D012701:Serotonin; D005340:Fibrinogen