title: Specific suppressor T cells in rats active in the afferent phase of contact hypersensitivity.
abstract: The optimal conditions for the induction of contact hypersensitivity in rats and the characteristics of its suppression were studied using the sensitizing haptens dinitrofluorobenzene (DNFB) and trinitrochlorobenzene (TNCB). The hypersensitivity was shown to be hapten specific in so far as TNCB did not sensitize for DNFB responses but sensitization with DNFB did allow a marginal response in rats challenged with TNCB. Suppression of the sensitization to DNFB and TNCB could be generated by intravenous injection of dinitrobenzenesulphonic acid (DNBS) or trinitrobenzenesulphonic acid (TNBS), respectively, up to 3 weeks before sensitization. This suppression was hapten specific and could be transferred with splenic T cells enriched for lymphocytes carrying the OX8 (Tc/s) cell marker. Only the induction phase of sensitization, however, could be suppressed in that way. No suppression acting upon the effector phase could be detected except for a nonspecific local suppression at the site of a previous challenge with an antigen to which the rat was specifically suppressed. This study shows that suppression of contact hypersensitivity in rats is mediated by specific suppressor T cells of which the activation pathway apparently differs from that postulated for mice.
mesh_terms: D000818:Animals; D003877:Dermatitis, Contact / Q000276:immunology*; D004139:Dinitrofluorobenzene / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D007116:Immunization, Passive; D007165:Immunosuppression Therapy; D051379:Mice / Q000276:immunology; D010853:Picryl Chloride / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains / Q000276:immunology; D013045:Species Specificity; D050378:T-Lymphocytes, Regulatory / Q000145:classification / Q000276:immunology* / Q000637:transplantation; D014302:Trinitrobenzenesulfonic Acid / Q000276:immunology
chemical_list: D000939:Epitopes; D014302:Trinitrobenzenesulfonic Acid; D004139:Dinitrofluorobenzene; D010853:Picryl Chloride