title: Antibody probing of western blots which have been stained with india ink.
abstract: India ink can be used to stain proteins bound to nitrocellulose. Subsequently, specific proteins can be identified with antibodies and 125I-protein A. In most cases, india ink did not significantly inhibit detection with antibody, nor was the ink washed off during the antibody incubation steps. Using this method, a direct comparison of antibody-reactive protein and total protein can be made with the same replica.
mesh_terms: D000906:Antibodies; D002244:Carbon; D004396:Coloring Agents; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D011506:Proteins / Q000032:analysis*; D013194:Staining and Labeling
chemical_list: D000906:Antibodies; D004396:Coloring Agents; D011506:Proteins; C028433:chinese ink; D002244:Carbon