Adaptation of the ASTRIN staining technique for quantitative determination of the functional state of the cellular nucleus. Experiments on the nucleoli of CHO cells.
Cytophotometric determination between 516 less than or equal to lambda less than or equal to 745 nm of the nucleolar spectra of cells stained with the ASTRIN technique has enabled the quantitative assessment of stain binding affinity and its changes. The hormone-like active molecule gamma-L-glutamyl-taurine, which acts at the nuclear level, gave rise to nucleolar activation, which could be precisely followed up by ASTRIN staining. It appears that the ASTRIN staining technique is suitable for the quantitative determination of changes in the functional state of the nucleus and nucleolus.
D000818:Animals; D002460:Cell Line; D002466:Cell Nucleolus / Q000502:physiology*; D002467:Cell Nucleus / Q000502:physiology*; D006224:Cricetinae; D005973:Glutamine / Q000031:analogs & derivatives*; D006651:Histocytochemistry; D013194:Staining and Labeling; D013654:Taurine / Q000031:analogs & derivatives*
D005973:Glutamine; D013654:Taurine; C020903:glutaurine