Microelectrode measurement of cell membrane potential in isolated hepatocytes attached to collagen.
Isolated spherical rat hepatocytes attached to collagen-coated cover slips generate a mean membrane potential (Em) of -78 +/- 9 mV as measured with high-resistance microelectrodes. The recordings were biphasic and were stable for upto 20 minutes. The correlation between external potassium concentration and Em was not linear. Several potassium-channel blockers did not effect the membrane potential. Addition of ouabain added to the incubation solution slowly depolarized the cells. The results indicate a high potassium permeability of the isolated spherical hepatocytes attached to collagen.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002462:Cell Membrane / Q000502:physiology*; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D003094:Collagen; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology; D008099:Liver / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects; D008839:Microelectrodes; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D051381:Rats
D007473:Ion Channels; D010042:Ouabain; D003094:Collagen; D011188:Potassium; D002118:Calcium