title: Different expression of alkaline phosphatase in subclones of human neoplastic salivary duct cell line.
abstract: Human neoplastic salivary cell line (HSGc) and its subclones express alkaline phosphatase (AP) which is sensitive to L-phenylalanine but insensitive to L-homoarginine. Electrophoretic analysis demonstrates two distinct bands of AP, one (sb-1) is heat-stable and another (sb-2) is rather heat-labile and faster mobility than sb-1. The AP activity, especially sb-2, shows high level in less-differentiated clone (HSGc-C5) with increased growth rate but low level in nontumorigenic and well-differentiated clone (HSGc-E1) as compared to parent HSGc. This may suggest that the AP is a possible marker for identification of undifferentiated state in HSGc. This study also indicates that dibutyryl cAMP produces an increase of heat-labile AP in these clones.
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis* / Q000378:metabolism; D002460:Cell Line; D002999:Clone Cells; D006709:Homoarginine / Q000494:pharmacology; D006358:Hot Temperature; D006801:Humans; D007527:Isoenzymes / Q000096:biosynthesis / Q000378:metabolism; D007700:Kinetics; D007930:Leucine / Q000494:pharmacology; D010649:Phenylalanine / Q000494:pharmacology; D012468:Salivary Gland Neoplasms / Q000201:enzymology*
chemical_list: D007527:Isoenzymes; D006709:Homoarginine; D010649:Phenylalanine; D000469:Alkaline Phosphatase; D007930:Leucine