Bleomycin-induced DNA-strand damage in isolated male germ cells.
DNA strand damage in isolated male germ cells (MGC) was evaluated after in vitro exposure to bleomycin (BLM), a known genotoxin. The alkaline elution technique was used to determine DNA-strand breaks. Concentration-dependent strand damage was established following exposure to bleomycin for 1 h at 37 degrees C. Exposure at 0 degrees C resulted in an increase in the frequency of strand breaks as compared to those observed at 37 degrees C. Pretreatment of cells with deferoxamine (DM), an iron-selective chelating agent, abolished the DNA damage induced by bleomycin. Isolated male germ cells responded in a predictable and reproducible manner thus supporting their use in mechanistic studies of genotoxicity.
D000818:Animals; D001761:Bleomycin / Q000633:toxicity*; D004247:DNA / Q000235:genetics*; D008297:Male; D009152:Mutagenicity Tests; D009154:Mutation; D051381:Rats; D013093:Spermatogonia / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects*; D013696:Temperature; D013737:Testis / Q000187:drug effects
D001761:Bleomycin; D004247:DNA