Characterization of established epithelioid cell lines derived from rat liver: expression of cytokeratin filaments.
A number of epithelioid cell lines were established from livers of fetal and neonatal rats. The clear-epithelial cells of these lines are non-neoplastically altered, clonogenic, nearly-diploid and showed no hepatocyte-like properties. The distribution and organization of intermediate filaments were analysed by indirect immunofluorescence and immunoperoxidase techniques using polyclonal and monoclonal antibodies against cytokeratins and vimentin. The different keratin antibodies also react strongly with intermediate filaments of bile duct cells on frozen sections of fetal and neonatal liver. Several subcultures of all established cell lines contained cell populations which reacted positively with antibodies against prekeratin and broad-range cytokeratins in a different manner, but not with antibodies against cytokeratin No. 19 of the catalogue of MOLL. The intermediate filaments are arranged in predominantly stained pericellular rings as well as in fine filamentous networks more evenly distributed throughout the cytoplasm. In early passages almost all cells were found positive for pre- and cytokeratins whereas in later subcultures the number of positive cells decreases and the expression of cytokeratin polypeptides varies considerably as seen with the different antibodies. All cells of the different cell lines show a well developed fine filamentous vimentin network extending throughout the whole cell up to the outer cell margin. Our findings support the concept that the clear epithelioid cells in established cell lines are of epithelial nature but they are not derived from biliary epithelium, and therefore probably immature parenchymal liver cells.
D000818:Animals; D000831:Animals, Newborn; D002460:Cell Line; D003599:Cytoskeleton / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D005333:Fetus; D007382:Intermediate Filaments / Q000378:metabolism*; D007633:Keratins / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D014746:Vimentin / Q000378:metabolism*
D014746:Vimentin; D007633:Keratins