A 60-kDa polypeptide in mammalian cells with epitopes related to actin.
We have identified a novel actin-related 60-kDa polypeptide in mammalian cells. The relatedness of this polypeptide to actin is indicated by its affinity for DNase I, two monoclonal anti-actin antibodies, and two independent peptide-specific anti-actin antibodies which bind to actin at around amino acid 244. It is not incorporated into cytoskeletal stress fibers, although it is a stable protein. Its expression (60-kDa polypeptide, pI of 5.4 to 5.5) is inhibited by the K+ ionophore, nonactin, which is known to collapse the energy-dependent translocation of cytoplasmically synthesized proteins into mitochondria.
D000199:Actins / Q000276:immunology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003850:Deoxyribonuclease I / Q000378:metabolism; D000939:Epitopes; D006801:Humans; D007163:Immunosorbent Techniques; D007526:Isoelectric Point; D018942:Macrolides; D008928:Mitochondria / Q000378:metabolism; D008970:Molecular Weight; D051381:Rats; D012995:Solubility
D000199:Actins; D000900:Anti-Bacterial Agents; D000911:Antibodies, Monoclonal; D000939:Epitopes; D018942:Macrolides; D003850:Deoxyribonuclease I; C050476:nonactin