Hyperthermia inhibition of tumor cells growth in the presence of ruthenium red.
Ruthenium red (RR) is taken up by DS sarcoma cells as a linear function of its concentration. Higher temperatures produce a higher uptake. This increased uptake is coincident with an augmentation of Trypan blue inclusion. A diminution of calcium transport in the presence of RR is observed at hyperthermia, and its uptake is not linear but presents a plateau for all temperatures and concentrations. RR-cell membrane interaction seems to produce two different effects: an early blocking of calcium transport, and a later change of the physicochemical characteristics of the plasma membrane. On the colony forming ability of CA1 cell RR shows clearly an intrinsic toxic effect, and when present during hyperthermia a low but significant enhancement of tumor cells mortality.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects; D006979:Hyperthermia, Induced; D008545:Melanoma / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D012428:Ruthenium / Q000494:pharmacology*; D012430:Ruthenium Red / Q000378:metabolism / Q000494:pharmacology*; D012513:Sarcoma, Experimental / Q000628:therapy*
D012430:Ruthenium Red; D012428:Ruthenium; D002118:Calcium