Detection of endotoxin in mice by measurement of endotoxin-induced changes in plasma concentrations of zinc and of the acute-phase protein serum amyloid P-component.
Preparations of six bacterial endotoxins reduced plasma concentrations of zinc and increased concentrations of the acute-phase protein serum amyloid P component (SAP) in mice. The changes were sufficiently sensitive to permit use of either response in tests for endotoxin and hence for pyrogenic contamination.
D000209:Acute-Phase Proteins / Q000032:analysis*; D000818:Animals; D004731:Endotoxins / Q000097:blood* / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D000683:Serum Amyloid P-Component / Q000032:analysis*; D013312:Stress, Physiological / Q000097:blood; D015032:Zinc / Q000097:blood*
D000209:Acute-Phase Proteins; D004731:Endotoxins; D000683:Serum Amyloid P-Component; D015032:Zinc