title: Silver staining of native and denatured eucaryotic DNA in agarose gels.
abstract: A modified method of silver staining for native and denatured eucaryotic DNA in 1% agarose gel is described. This method is at least fivefold more sensitive than ethidium bromide staining, with a detection limit of 2.5 ng for total DNA. The calibration curve is linear within the range 5-30 ng of single-stranded and double-stranded DNA. This method is especially advantageous for electrophoretic assessment of DNA molecular weights.
mesh_terms: D000818:Animals; D004247:DNA / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D004996:Ethidium; D005057:Eukaryotic Cells; D009691:Nucleic Acid Denaturation; D012474:Salmon / Q000378:metabolism; D012834:Silver; D013194:Staining and Labeling
chemical_list: D012834:Silver; D004247:DNA; D004996:Ethidium