A rapid method for determining soot content of lungs in diesel-exposed rodents.
A rapid optical method for determining the quantity of soot in the lungs of rodents exposed to diluted diesel exhaust has been developed. The method is nondestructive to enzymatic components of the lung, allowing dose and biochemical assays to be done on the same sample. The lung burdens of soot were determined by comparing the extinction of light at 620 nm of lung homogenates from exposed animals to that of lung homogenates containing spiked standards.
D000393:Air Pollutants / Q000032:analysis*; D000818:Animals; D002244:Carbon / Q000032:analysis*; D005260:Female; D008168:Lung / Q000032:analysis*; D008297:Male; D051381:Rats; D001335:Vehicle Emissions
D000393:Air Pollutants; D001335:Vehicle Emissions; D002244:Carbon