Hybrid polypeptide heavy chains produced by two hybridoma lines.
Two anti-TNP antibodies exhibiting unusual features are described. They were obtained in two independent fusions. Spleen cells from CB20 mice sensitized with TNP-Ficoll and challenged with TNP-LPS were fused with SP2/0 myeloma cells. One of these hybridomas, CBT3, secretes antibodies which react with both monospecific anti-gamma 2b and anti-gamma 3 anti-isotypic sera; the second hybridoma, CBT4, secretes antibodies reacting with monospecific anti-mu and anti-gamma 2b sera. Only one type of immunoglobulin is secreted by each hybridoma, ruling out the hypothesis of hybrid molecules formed by distinct heavy chains. These results imply that the two heavy chains are made up from elements encoded by gamma 3 and gamma 2b genes in CBT3 and by gamma 2b and mu genes in CBT4. The molecular mechanisms underlying the production of these singular heavy chains are discussed.
D000818:Animals; D002460:Cell Line; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D000939:Epitopes / Q000032:analysis; D006825:Hybridomas / Q000276:immunology*; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis*; D007132:Immunoglobulin Isotypes / Q000032:analysis; D051379:Mice; D008815:Mice, Inbred Strains; D055503:Protein Multimerization; D014301:Trinitrobenzenes / Q000276:immunology
D000939:Epitopes; D007143:Immunoglobulin Heavy Chains; D007132:Immunoglobulin Isotypes; D014301:Trinitrobenzenes