Quantitation of chick tissue zinc-metallothionein by gel electrophoresis and silver stain enhancement.
Discontinuous gradient gel electrophoretic systems were developed to quantitate zinc-metallothionein (Zn-MT) in chick tissues, liver and pancreas. Gels were stained with Coomassie Blue initially, then enhanced by silver stain. At least 4 micrograms of Zn-MT could be detected after Coomassie Blue stain, and 1 microgram Zn-MT detected following silver stain enhancement. Significant linearity (correlation coefficient = 0.99) of a standard curve was established in the Coomassie Blue stained gels. The results of our experiment suggest that electrophoretic analysis is a simple and feasible method for the quantitation and identification of Zn-MT in chick tissues.
D000818:Animals; D002645:Chickens; D003600:Cytosol / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000032:analysis; D008297:Male; D008668:Metallothionein / Q000032:analysis*; D010179:Pancreas / Q000032:analysis; D012834:Silver; D013194:Staining and Labeling
D012834:Silver; D008668:Metallothionein