Purification and reconstitution of the calcium release channel from skeletal muscle.
The calcium release channel from rabbit muscle sarcoplasmic reticulum (SR) has been purified and reconstituted as a functional unit in lipid bilayers. Electron microscopy reveals the four-leaf clover structure previously described for the 'feet' that span the transverse tubule (T)-SR junction. Ca2+ release from the SR induced by T-system depolarization during excitation-contraction coupling in muscle may thus be effected through a direct association of the T-system with SR Ca2+-release channels.
D000818:Animals; D002118:Calcium / Q000378:metabolism; D007473:Ion Channels / Q000032:analysis* / Q000378:metabolism / Q000648:ultrastructure; D008081:Liposomes; D008564:Membrane Potentials; D011817:Rabbits; D011950:Receptors, Cholinergic / Q000302:isolation & purification* / Q000378:metabolism; D019837:Ryanodine Receptor Calcium Release Channel; D012519:Sarcoplasmic Reticulum / Q000032:analysis* / Q000648:ultrastructure
D007473:Ion Channels; D008081:Liposomes; D011950:Receptors, Cholinergic; D019837:Ryanodine Receptor Calcium Release Channel; D002118:Calcium