Binding of monoclonal anti-myelin-associated glycoprotein antibodies to human foetal peripheral neurons in culture.
Dorsal root ganglion cells, obtained from 8-10-week human foetuses, were isolated by enzymatic procedure and grown on poly-L-lysine-coated coverslips. Most of the cultured cells showed the ultrastructural and immunological features of normal peripheral neurons. By immunocytochemistry neurons reacted with IgM antibodies with specificity for myelin-associated glycoprotein (MAG) from patients affected with IgM k gammopathy and peripheral neuropathy. The antigen was located on the plasmalemma of both perikarion and axon. We suggest that anti-MAG antibodies do not recognize neuronal MAG, but rather an epitope shared with different glycoproteins.
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000276:immunology*; D015815:Cell Adhesion Molecules; D046508:Culture Techniques; D000939:Epitopes / Q000276:immunology; D005333:Fetus; D005455:Fluorescent Antibody Technique; D005727:Ganglia, Spinal / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D009185:Myelin Proteins / Q000276:immunology*; D018992:Myelin-Associated Glycoprotein
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D000939:Epitopes; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein