Production of monoclonal antibodies to metacyclic trypomastigotes of Trypanosoma cruzi.
Monoclonal antibodies were produced and characterized to the metacyclic trypomastigotes stage of Trypanosoma cruzi. All the monoclonal antibodies (GR-12C/5; GR-P2B/5; GR-F2B/1; GR-J2C/6; GR-E6B/3; GR-A5B/5) also recognized antigens of epimastigotes of T. cruzi. These antigens are associated with the plasma membrane, flagellum, and an intracellular structure located by the nucleus. Immunoprecipitation using the monoclonal antibodies GR-J2C/6 and GR-E6B/3 followed by SDS-PAGE analysis has led to the following results: GR-J2C/6 precipitated two molecules with apparent molecular weights of 58 and 60 kD; the antigen recognized by GR-E6B/3 was a molecule with molecular weight of 68 kD. Only two of the monoclonal antibodies (GR-12C/5 and GR-P2B/5) recognized antigens of promastigotes of both Leishmania mexicana and L. infantum, and are associated with the plasma membrane.
D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification; D000953:Antigens, Protozoan / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D005779:Immunodiffusion / Q000379:methods; D007893:Leishmania donovani / Q000254:growth & development / Q000276:immunology; D007894:Leishmania mexicana / Q000254:growth & development / Q000276:immunology; D008970:Molecular Weight; D011233:Precipitin Tests; D014349:Trypanosoma cruzi / Q000254:growth & development / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D000939:Epitopes