title: Heat shock phenomena in Aspergillus nidulans. II. Combined effect of heat and bleomycin to heat shock protein synthesis, survival rate and induction of mutations.
abstract: The combined action of hyperthermia and Bleomycin on Aspergillus nidulans was studied at three different levels: mycelial protein synthesis, spore viability and induction of mutations. It was found that Bleomycin treatment of preincubated mycelia during the heat shock enhances the incorporation of 35S-methionine into heat shock bands. Furthermore, simultaneous treatment with hyperthermia (43 degrees C) and Bleomycin results in greater cytotoxic activity in spores and in a higher induction rate of point mutations.
mesh_terms: D001233:Aspergillus nidulans / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D001761:Bleomycin / Q000494:pharmacology*; D005656:Fungal Proteins / Q000096:biosynthesis; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D006358:Hot Temperature; D009154:Mutation
chemical_list: D005656:Fungal Proteins; D006360:Heat-Shock Proteins; D001761:Bleomycin