Impaired ristocetin aggregation in myeloma due to binding of the aggregating agent by monoclonal protein--presentation of a case.
In a case of severe IgG kappa myeloma with cryoglobulinaemia usual concentrations of epinephrine, collagen, ADP, arachidonic acid, thrombin and ristocetin caused no aggregation of platelets in platelet rich plasma. However, in contrast to other agents ristocetin induced platelet aggregation in higher concentrations. The investigations showed, that the aggregating activity was inhibited by binding of ristocetin to the abnormal protein. Following saturation of the monoclonal protein, the surplus ristocetin caused normal aggregation. This indicates that platelets actually preserved their responsiveness to ristocetin. Possible causes of the phenomenon are discussed.
D000368:Aged; D003449:Cryoglobulinemia / Q000097:blood; D005260:Female; D006474:Hemorrhagic Disorders / Q000097:blood*; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D007145:Immunoglobulin kappa-Chains / Q000378:metabolism; D009101:Multiple Myeloma / Q000097:blood*; D010974:Platelet Aggregation / Q000187:drug effects*; D011485:Protein Binding; D012310:Ristocetin / Q000097:blood; D014842:von Willebrand Diseases / Q000097:blood
D007074:Immunoglobulin G; D007145:Immunoglobulin kappa-Chains; D012310:Ristocetin