title: Accumulation of a precursor of subunit S1 of pertussis toxin in cell envelopes of Bordetella pertussis in response to the membrane perturbant phenethyl alcohol.
abstract: Exponential cultures of Bordetella pertussis strain 18334 were treated with the membrane-perturbing agent phenethyl alcohol which, at a concentration of 0.075% v/v, blocked the synthesis of mature subunit S1 of pertussis toxin as revealed by Western blotting. It also caused the accumulation of a precursor, pS1, with an estimated mol. wt of 32 X 10(3), that was located in the cytoplasmic membrane. These findings suggested that subunit S1 of pertussis toxin was exported in a signal peptide-dependent manner.
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D001886:Bordetella pertussis / Q000032:analysis* / Q000187:drug effects; D002462:Cell Membrane / Q000032:analysis; D002499:Centrifugation, Density Gradient; D000431:Ethanol / Q000031:analogs & derivatives*; D037342:Pertussis Toxin; D010626:Phenylethyl Alcohol / Q000494:pharmacology*; D011498:Protein Precursors / Q000032:analysis*; D010566:Virulence Factors, Bordetella / Q000096:biosynthesis*
chemical_list: D001426:Bacterial Proteins; D011498:Protein Precursors; D010566:Virulence Factors, Bordetella; D000431:Ethanol; D037342:Pertussis Toxin; D010626:Phenylethyl Alcohol