title: Elimination and restoration of voltage dependence in the mitochondrial channel, VDAC, by graded modification with succinic anhydride.
abstract: The major permeability pathways of the outer mitochondrial membrane are the voltage-gated channels called VDAC. It is known that the conductance of these channels decreases as the transmembrane voltage is increased in the positive or negative direction. These channels are known to display a preference for anions over cations of similar size and valence. It was proposed (Doring & Colombini, 1985b) that a set of positive charges lining the channel may be responsible for both voltage dependence and selectivity. A prediction of this proposal is that progressive replacement of the positive charges with negative charges should at first diminish, and then restore, voltage dependence. At the same time, the channel's preference for anions over cations should diminish then reverse. Succinic anhydride was used to perform these experiments as it replaces positively charged amino groups with negatively charged carboxyl groups. When channels, which had been inserted into phospholipid membranes, were treated with moderate amounts of the anhydride, they lost their voltage dependence and preference for anions. With further succinylation, voltage dependence was regenerated while the channels became cation selective. The voltage needed to close one-half of the channels increased in those treatments in which voltage dependence was diminished. As voltage dependence was restored, the voltage needed to close half of the channels decreased. The energy difference between the open and closed state in the absence of an applied field changed little with succinylation, indicating that the procedure did not cause large changes in VDAC's structure but specifically altered those charges responsible for voltage gating and selectivity.
mesh_terms: D007425:Intracellular Membranes / Q000502:physiology*; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D008433:Mathematics; D008564:Membrane Potentials; D008928:Mitochondria / Q000502:physiology*; D008954:Models, Biological; D009492:Neurospora crassa / Q000502:physiology; D013386:Succinates / Q000494:pharmacology*; D013387:Succinic Anhydrides / Q000494:pharmacology*; D013816:Thermodynamics
chemical_list: D007473:Ion Channels; D013386:Succinates; D013387:Succinic Anhydrides; C031801:succinic anhydride