The relationship between cell proliferation and phosphatidylinositol metabolism in skeletal muscle cells in culture.
Effects of gamma-hexachlorocyclohexane (1 X 10(-4) M) and lithium chloride (10 mM) on the cell proliferation and phosphatidylinositol metabolism of L6 myoblasts were examined. gamma-Hexachlorocyclohexane (inhibitor of phosphatidylinositol synthesis) and LiCl (inhibitor of inositol 1-phosphatase) treatment of these cells resulted in the loss of their proliferative properties. Coordinate with effects on cell proliferation, gamma-hexachlorocyclohexane and LiCl also reduced the levels of phosphatidylinositols and their water-soluble metabolites. However, the protein content of treated cells was higher than control cells. Gamma-hexachlorocyclohexane and LiCl treatment of cells reduced DNA synthesis by about 50% over control values. It was, therefore, concluded that there appears to be a direct relationship between L6 myoblast proliferation and phosphatidylinositol metabolism.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002712:Chlorides / Q000494:pharmacology; D001556:Hexachlorocyclohexane / Q000494:pharmacology; D008094:Lithium / Q000494:pharmacology; D018021:Lithium Chloride; D009132:Muscles / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism*; D013552:Swine
D002712:Chlorides; D010716:Phosphatidylinositols; D001556:Hexachlorocyclohexane; D008094:Lithium; D018021:Lithium Chloride