Repair processes of hemopoiesis after applying cyclophosphamide. II. Quantitative changes in basic proteins and DNA in the bone marrow, spleen, and thymus.
The effect of cyclophosphamide on the organ weight, DNA and basic protein content in nuclei of bone marrow, thymus and spleen of rats was studied. Animals were examined 6, 12 and 24 hours and 3, 5, 10, 20 and 30 days after intraperitoneal application of cyclophosphamide in the doses of 100 and 200 mg X kg-1 of body weight. From the 5th day after application a marked decrease in weight and cellularity of all organs was observed. Amounts of DNA and basic proteins in nuclei of bone marrow and spleen were higher as compared with controls and their increase occurred prior to recovery of cellularity and organ weight, that was initiated within 5 and 10 days after cyclophosphamide treatment. Changes in the thymus persisted for a longer period of time and recovery was incomplete even at the day 30 after cyclophosphamide application. In accordance with these data no increase in DNA and basic protein amounts in the thymus nuclei was observed.
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects*; D002467:Cell Nucleus / Q000032:analysis; D003520:Cyclophosphamide / Q000494:pharmacology*; D004247:DNA / Q000032:analysis*; D006410:Hematopoiesis / Q000187:drug effects*; D008297:Male; D009929:Organ Size; D011506:Proteins / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000187:drug effects*; D013950:Thymus Gland / Q000187:drug effects*
D011506:Proteins; D003520:Cyclophosphamide; D004247:DNA