title: Assay of urinary 5-hydroxyindole-3-acetic acid: two methodologies compared.
abstract: Udenfriend's (Sjoerdsma A, Weissbach H, Udenfriend S. J Am Med Assoc 1955; 159: 397) classical screening method for urinary 5-hydroxyindole-3-acetic acid (5-HIAA) is known to be subject to error due to food and drug interferences. Goldenberg (Goldenberg H. Clin Chem 1973; 19: 38-44) introduced a more specific and sensitive method, which has recently been modified by Zouheir Habbal (Zouheir Habbal M. Clin Chim Acta 1983; 130: 251-256). The latter method is shown to correlate fairly well with a high-pressure liquid chromatography (HPLC) method in the 0-100 mumol/24 h range (reference values up to 34 mumol/24 h). It is suggested that elevated values in Udenfriend's screening method can be reliably checked by applying either method.
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000652:urine*; D013050:Spectrometry, Fluorescence
chemical_list: D006897:Hydroxyindoleacetic Acid