The search for the DNA of the chick embryo fibroblast cytosolic complex.
1. Conventional DNA extraction procedures have failed to release free DNA from the chick embryo fibroblast cytosolic DNA-RNA complexes. 2. Free DNA has been released only from the smallest cell cytosol DNA fraction, which is not in the native state associated with RNA: it is very small (of the order of 100 bases) and single stranded. 3. However, phenol extraction does separate complex DNA-associated material from the RNA which has invariably been found to accompany it in all but the smallest fraction (see 2 above). 4. The principal factor preventing DNA release appears to be a massive aggregation of partially purified DNA-associated material.
D000818:Animals; D002642:Chick Embryo; D003600:Cytosol / Q000032:analysis*; D004247:DNA / Q000302:isolation & purification* / Q000378:metabolism; D005347:Fibroblasts / Q000032:analysis*; D019800:Phenol; D010636:Phenols; D011494:Protein Kinases; D012313:RNA / Q000378:metabolism
D010636:Phenols; D019800:Phenol; D012313:RNA; D004247:DNA; D011494:Protein Kinases