Metabolism of biogenic aldehydes in human blood: effects of ethanol, acetaldehyde and disulfiram.
The metabolism of biogenic aldehydes was measured in different fractions of human blood. Isolated erythrocytes, leukocytes, platelets and plasma were incubated with the aldehydes derived from dopamine and serotonin (DOPAL and 5-HIAL, respectively). The disappearance of the aldehydes and the formation of the acid and alcohol metabolites were analysed using HPLC with electrochemical detection. When erythrocytes were incubated with 1 microM DOPAL or 5-HIAL only the acid metabolites were formed, whereas both acid and alcohol metabolites were formed in incubations with leukocytes or platelets. No metabolites were formed when the aldehydes were incubated with plasma. In incubations with erythrocytes, the oxidation of the aldehydes was totally inhibited in the presence of 50 microM disulfiram, whereas the formation of both acid and alcohol metabolites was inhibited in incubations with platelets. Disulfiram did not affect the metabolism of DOPAL or 5-HIAL in incubations with leukocytes. The oxidation of the aldehydes was inhibited by 50-70% in the presence of a 500-fold excess of acetaldehyde. Ethanol at a concentration of 10 mM did not affect the metabolism.
D015102:3,4-Dihydroxyphenylacetic Acid / Q000031:analogs & derivatives / Q000097:blood / Q000378:metabolism; D000079:Acetaldehyde / Q000494:pharmacology*; D000447:Aldehydes / Q000097:blood / Q000378:metabolism*; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D004221:Disulfiram / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D000431:Ethanol / Q000494:pharmacology*; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000031:analogs & derivatives / Q000097:blood / Q000378:metabolism; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism
D000447:Aldehydes; D015102:3,4-Dihydroxyphenylacetic Acid; C004277:hydroxyindoleacetaldehyde; D000431:Ethanol; D006897:Hydroxyindoleacetic Acid; C007430:3,4-dihydroxyphenylacetaldehyde; D000079:Acetaldehyde; D004221:Disulfiram