title: Developmental changes in the molecular weights of polypeptides in the human CNS that carry the HNK-1 epitope and bind Phaseolus vulgaris lectins.
abstract: The binding patterns of electrophoresed polypeptides from homogenates of human frontal lobe, cerebellum, and spinal cord obtained at various stages of development were determined for several lectins with specificities for a wide range of oligosaccharides. A discrete developmental change in the molecular-weight pattern was seen only among polypeptides binding the two Phaseolus vulgaris agglutinins, E-phytohemagglutinin (E-PHA) and L-PHA. With increasing maturity, the apparent molecular weights of the major polypeptides binding these two lectins progressively decreased. Furthermore, at all stages of development, E-PHA and L-PHA bound to the same polypeptides as the monoclonal antibody HNK-1, which recognizes a carbohydrate epitope on polypeptides that may play roles in cell adhesion. Based on the carbohydrate specificities of the two PHAs, we conclude that it is likely that the HNK-1 epitope resides on a triantennary N-linked oligosaccharide bisected by N-acetylglucosamine.
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal; D002531:Cerebellum / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis* / Q000276:immunology; D005625:Frontal Lobe / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism; D006801:Humans; D007223:Infant; D037102:Lectins / Q000378:metabolism; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000276:immunology / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism; D010835:Phytohemagglutinins / Q000378:metabolism*; D013116:Spinal Cord / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D037102:Lectins; D009419:Nerve Tissue Proteins; D009844:Oligosaccharides; D010835:Phytohemagglutinins