[The role of phosphoric acid residues in the formation of antigenic determinants of the structural components of DNA].
The inhibitory activity of thymidine, thymidine triphosphate and thymidyl oligonucleotides was studied in thymidine-antithymidine antibodies reaction. Thymidine was shown to have the greatest inhibitory effect, with thymidine triphosphate and thymidyl oligonucleotide inhibitory activity less expressed and reducing with the increase in oligonucleotide length. The effect of thymidine, thymidine triphosphate and thymidyl oligonucleotides on the interaction of antisera and SLE patients' sera with denatured DNA was studied. It was shown that thymidine triphosphate and particularly thymidyl oligonucleotides are characterized by greater inhibitory capacity, as compared to thymidine. It was found that only thymine dimers bound by phosphate groups can inhibit the interaction of UV-irradiated DNA with antiserum specific for UV-modified DNA. The data obtained suggest that the charge determined by phosphoric acid residues plays an essential role in the interaction of antibodies induced to charged structural DNA components.
D000974:Antibodies, Antinuclear / Q000276:immunology*; D000937:Antigen-Antibody Reactions / Q000187:drug effects; D004247:DNA / Q000276:immunology*; D000939:Epitopes / Q000032:analysis*; D006801:Humans; D009841:Oligonucleotides / Q000494:pharmacology; D010756:Phosphoric Acids / Q000032:analysis* / Q000276:immunology; D013942:Thymine Nucleotides / Q000494:pharmacology
D000974:Antibodies, Antinuclear; D000939:Epitopes; D009841:Oligonucleotides; D010756:Phosphoric Acids; D013942:Thymine Nucleotides; D004247:DNA; C030242:phosphoric acid
Znachenie ostatkov fosfornoĭ kisloty v formirovanii antigennoĭ determinanty strukturnykh komponentov DNK.