Fluorescent staining of proteins transferred to nitrocellulose allowing for subsequent probing with antisera.
A sensitive staining method for protein blots on nitrocellulose is described. It is based on the coupling of a fluorochrome, dichlorotriazynylaminofluorescein, to protein which yields products colorless in visible light but colored when protein blots are illuminated with long-range ultraviolet light. The coupling of a fluorochrome does not affect the antigenic properties of proteins and the stained blots can be subsequently probed with antisera. Thus, the method allows for the unambiguous identification of antigenic proteins transferred to nitrocellulose from sodium dodecyl sulfate-polyacrylamide gels.
D000906:Antibodies; D003101:Collodion; D005452:Fluoresceins; D011506:Proteins / Q000032:analysis* / Q000276:immunology; D013194:Staining and Labeling / Q000379:methods*
D000906:Antibodies; D005452:Fluoresceins; D011506:Proteins; C039083:5-((4,6-dichloro-1,3,5-triazin-2-yl)amino)fluorescein; D003101:Collodion