Induction of HLA-DR antigen expression in human endometrial epithelial cells in vitro by recombinant gamma-interferon.
Recent studies suggest that HLA-DR antigens may be inducible in some normal epithelial cells. Therefore, we studied the expression of these molecules in epithelial cell cultures derived from 9 proliferative and 7 secretory endometria with and without treatment by recombinant gamma-interferon (gamma-IFN). A panel of monoclonal antibodies to HLA-DR antigens, T cells, B cells, macrophages, endothelial cells, and cytokeratin was used to stain the cultured cells by the avidin-biotin complex method. The cultures consisted only of epithelial cells as demonstrated by expression of cytokeratin and by electron microscopy and did not contain any T or B cells, macrophages, or endothelial cells. The endometrial epithelial cells were HLA-DR-negative under routine culture conditions but expressed HLA-DR antigens after gamma-IFN treatment in a dose- and time-dependent fashion. The findings indicate that endometrial epithelium can actively synthesize HLA-DR antigens in vitro after induction by gamma-IFN.
D002478:Cells, Cultured; D004717:Endometrium / Q000276:immunology*; D004848:Epithelium / Q000276:immunology; D005260:Female; D006681:HLA-D Antigens / Q000276:immunology*; D006684:HLA-DR Antigens / Q000276:immunology*; D006652:Histological Techniques; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology*; D011994:Recombinant Proteins; D013194:Staining and Labeling
D006681:HLA-D Antigens; D006684:HLA-DR Antigens; D011994:Recombinant Proteins; D007371:Interferon-gamma