[Acetylcholinesterase expression and changes in the electrical excitability of neuroblastoma cells during their morphological differentiation induced by an increase in the pH of the medium].
Inhibition of cell division and outgrowth of neurites with average rate of 31.5 +/- 4.4 micrometers per hour were observed in neuroblastoma cultures of the Neuro 2a clonal line 24 hours after the increase in the culture medium pH from 7.4 to 8.2. The total neurite length per one cell was about 298 +/- 36 micron in average by the 9-10th days of treatment. Simultaneously, a gradual enhancement of acetylcholinesterase cytochemical appearance took place attaining its maximum level by the same time. The peak sodium conductance, taken as a measure of sodium tetrodotoxin-sensitive potential-dependent channel density, was the same both in nondifferentiated cells grown in suspension or monolayer cultures, and in morphologically differentiated ones. The data lead to a conclusion that biochemical (acetylcholinesterase probe) and electrophysiological (sodium channel density) signs can express independently of morphological differentiation.
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation; D006651:Histocytochemistry; D006863:Hydrogen-Ion Concentration; D007473:Ion Channels / Q000502:physiology; D008564:Membrane Potentials; D051379:Mice; D009447:Neuroblastoma / Q000201:enzymology* / Q000503:physiopathology; D012964:Sodium / Q000378:metabolism; D013997:Time Factors; D014407:Tumor Cells, Cultured
D007473:Ion Channels; D012964:Sodium; D000110:Acetylcholinesterase
Ekspressiia atsetilkholinésterazy i izmeneniia élektrovozbudimosti v kletkakh neĭroblastomy v khode ikh morfologicheskoĭ differentsirovki, indutsirovannoĭ povysheniem pH sredy.