[Electrophoretic purification of biologically active structural proteins of the simian adenovirus SA7 in the presence of sodium dodecyl sulfate].
The modification of disc electrophoresis technique in polyacrylamide gel with sodium dodecylsulphate (SDS) has been elaborated for synchronous isolation of some structural proteins in biologically active form and in preparative quantities from adenoviruses. Virions of SA7 adenovirus were mildly dissociated in SDS solution at 20 degrees C and structural proteins were stained by fluorescamin. After separation the zones of proteins corresponding to the native capsomeres of hexon and protein IV as well as the zones of inner proteins V and VII have been identified as fluorescent at UV-irradiation, excised and extracted by SDS solution. After the removal of SDS by protein precipitation in acetone the preparations of hexon and IV reveal the quaternary structure of native capsomers and full spectrum of antigenic and immunogenic activities of native proteins. Preparations of inner proteins V and VII possess activity in condensing adenoviral DNA. The technique is usable for preparative purification of inner polypeptide VI SA7, as well as capsomers and inner proteins of other adenoviruses.
D000256:Adenoviridae / Q000032:analysis*; D000261:Adenoviruses, Simian / Q000032:analysis* / Q000276:immunology; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D012967:Sodium Dodecyl Sulfate; D014764:Viral Proteins / Q000276:immunology / Q000302:isolation & purification*; D015678:Viral Structural Proteins; D014771:Virion / Q000032:analysis* / Q000276:immunology
D000956:Antigens, Viral; D000939:Epitopes; D014764:Viral Proteins; D015678:Viral Structural Proteins; D012967:Sodium Dodecyl Sulfate; C028913:dodecyl sulfate
Elektroforeticheskaia ochistka biologicheski aktivnykh strukturnykh belkov adenovirusa obez'ian SA7 v prisutstvii dodetsilsul'fata natriia.