Peptidergic hypothalamic afferents to the cat nucleus raphe pallidus as revealed by a double immunostaining technique using unconjugated cholera toxin as a retrograde tracer.
Using a double immunostaining technique with unconjugated cholera toxin (CT) as a retrograde tracer, we have demonstrated in the cat that the nucleus raphe pallidus receives two major afferent projections from the hypothalamus: the preoptic periventricular nucleus; and the peri- and paraventricular zones of the posterior hypothalamic area. Some CT-labeled neurons in the preoptic periventricular nucleus showed Met-Enk-like immunoreactivity, while many CT-labeled neurons in the posterior hypothalamic area presented either corticotropin-releasing-factor-like or Met-Enk-like immunoreactivity.
D000344:Afferent Pathways / Q000502:physiology; D000818:Animals; D002415:Cats; D002772:Cholera Toxin; D007031:Hypothalamus / Q000502:physiology*; D007158:Immunologic Techniques; D010455:Peptides / Q000502:physiology*; D011903:Raphe Nuclei / Q000502:physiology*; D013194:Staining and Labeling; D009435:Synaptic Transmission
D010455:Peptides; D002772:Cholera Toxin