Atrial natriuretic polypeptide hormones induce membrane potential responses in cultured rat glioma cells.
Atrial natriuretic hormones (ANHs) applied to polyploid rat glioma cells induced hyperpolarizations of about 30 s duration, followed by depolarizations lasting 1-2 min. Repeated applications of the peptide resulted in desensitization. The reversal potential of -87 mV at an extracellular K+ concentration of 5 mM and the decrease of membrane resistance during the hyperpolarization indicate that K+ channels were activated by ANH. In these cells the fluorescence signal of 2-[(2-bis[carboxymethyl]amino-5-methylphenoxy)-methyl]-6-methoxy-8-bis [carboxymethyl]aminoquinoline (quin2) was not affected by ANH suggesting that ANH did not change the cytosolic Ca2+-activity.
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D001920:Bradykinin / Q000494:pharmacology; D002478:Cells, Cultured; D005910:Glioma; D006822:Hybrid Cells; D007473:Ion Channels / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects*; D009417:Nerve Tissue / Q000187:drug effects*; D009447:Neuroblastoma; D011188:Potassium / Q000378:metabolism; D051381:Rats
D007473:Ion Channels; D009320:Atrial Natriuretic Factor; D011188:Potassium; D001920:Bradykinin