Mechanically induced electrical responses in murine mammary epithelial cells in primary culture.
In mouse mammary epithelial cells in primary culture, mechanical stimulation of a cell induced in other cells within the same colony a short depolarization of less than 15 mV with a duration of 1-8 s and a subsequent, prominent hyperpolarization of 6 mV lasting 10-40 s. Epidermal growth factor induces a spontaneous hyperpolarizing response in cultured mammary cells, and in cells treated with EGF mechanical stimulation produced a greater hyperpolarization, while the amplitude of the depolarizing response was not affected. The amplitude of the mechanically induced hyperpolarization was markedly reduced by quinine and tetraethylammonium, blockers of the Ca2+ -dependent K+ channel. The results suggest that the Ca2+ -dependent K+ channel was involved in the hyperpolarization.
D000818:Animals; D002118:Calcium / Q000502:physiology; D002478:Cells, Cultured; D004594:Electrophysiology; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology; D007328:Insulin / Q000494:pharmacology; D007473:Ion Channels / Q000502:physiology; D008321:Mammary Glands, Animal / Q000166:cytology / Q000502:physiology*; D008564:Membrane Potentials; D051379:Mice; D011803:Quinine / Q000494:pharmacology; D013314:Stress, Mechanical; D013757:Tetraethylammonium Compounds / Q000494:pharmacology
D007328:Insulin; D007473:Ion Channels; D013757:Tetraethylammonium Compounds; D011803:Quinine; D002118:Calcium