title: Loss of secretory response of rat basophilic leukemia (2H3) cells at 40 degrees C is associated with reversible suppression of inositol phospholipid breakdown and calcium signals.
abstract: Antigen-induced stimulatory signals as well as histamine secretion from the RBL-2H3 cells were found to be highly temperature dependent. There was no hydrolysis of inositol phospholipids, increase in cytosol calcium concentration (calcium signal), or secretion upon antigen stimulation at temperatures below 20 degrees C. At higher temperatures (i.e., 20 to 37 degrees C), all responses increased in extent with increase in temperature. Temperatures of 38 degrees C or higher, however, resulted in a marked decline in all responses, until no responses were observed at 40 to 42 degrees C. As indicated by the decay in calcium signal, the duration of response was also temperature dependent. The response was of long duration at 30 to 32 degrees C, but it became progressively more transient as the temperature was increased from 32 to 40 degrees C. The effects of low or high temperature were fully reversible. For example, in the presence of antigen, stimulatory signals immediately appeared once the temperature was decreased from 40 to 37 degrees C. Although the diminished responses could be explained, in part, by a reduction in rates of IgE receptor aggregation and phospholipase C activity, the reductions were insufficient to account for complete loss of activity at 40 degrees C. We conclude that generation of intracellular signals in 2H3 cells is blocked by quite small elevations in temperature above 37 degrees C, possibly as consequence of changes in membrane fluidity.
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology; D001491:Basophils / Q000378:metabolism*; D002118:Calcium / Q000032:analysis*; D006636:Histamine Release; D006358:Hot Temperature; D006868:Hydrolysis; D007424:Intracellular Fluid / Q000032:analysis; D007942:Leukemia, Experimental / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000378:metabolism*; D051381:Rats; D011961:Receptors, Fc / Q000032:analysis; D017455:Receptors, IgE; D011971:Receptors, Immunologic / Q000032:analysis; D010738:Type C Phospholipases / Q000032:analysis
chemical_list: D000941:Antigens; D010716:Phosphatidylinositols; D011961:Receptors, Fc; D017455:Receptors, IgE; D011971:Receptors, Immunologic; D010738:Type C Phospholipases; D002118:Calcium