Agarose gel electrophoresis of denatured RNA with silver staining.
This paper describes agarose gel electrophoresis and silver staining of denatured RNAs. Glyoxal- or formaldehyde-denatured RNAs are electrophoresed in an agarose gel cast on a plastic support using an inert, low conductivity buffer. Following electrophoresis, the gel is stained with a sensitive silver stain. The method produces sharp, well-resolved bands and yields accurate RNA size estimates. Because of its sensitivity and simplicity, it is suitable for routine laboratory use.
D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli; D005557:Formaldehyde; D006037:Glyoxal; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D012313:RNA / Q000032:analysis*; D012834:Silver; D013194:Staining and Labeling
D005557:Formaldehyde; D012834:Silver; D006037:Glyoxal; D012313:RNA