A single RNA species injected in Xenopus oocyte directs the synthesis of active tyrosine hydroxylase.
Tyrosine hydroxylase, the rate limiting enzyme in the biosynthesis of catecholamine, is a tetramer composed of four subunits of the same molecular mass. A full length cDNA clone encoding tyrosine hydroxylase has been inserted into the SP6 expression system. Translation of the corresponding RNA in Xenopus oocyte results in enzymatic activity, demonstrating that a single gene contains all the necessary genetic information to code for a functional enzyme. The potential of this system in the analysis of posttranslational tyrosine hydroxylase modifications is discussed.
D000818:Animals; D003001:Cloning, Molecular; D004247:DNA; D066298:In Vitro Techniques; D009865:Oocytes / Q000201:enzymology*; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D012313:RNA / Q000502:physiology*; D014158:Transcription, Genetic; D014446:Tyrosine 3-Monooxygenase / Q000096:biosynthesis* / Q000235:genetics; D014982:Xenopus laevis
D012313:RNA; D004247:DNA; D014446:Tyrosine 3-Monooxygenase