The elimination of trypsin--alpha-macroglobulin complexes from the blood circulation in pigs during acute pancreatitis and after massive intravenous trypsin infusion.
The elimination from plasma of intravenously injected 125I-labelled trypsin-alpha-macroglobulin complexes was studied in both healthy and diseased, anaesthetized pigs. The t1/2 for the 125I-labelled complexes was 5-10 min in healthy pigs as well as in two groups of pancreatitis pigs. Pre-loading of the eliminating capacity by massive intravenous trypsin infusion, raised t1/2 to 80-100 min. Thus, the eliminating capacity seems to be saturable. Elimination of the complexes from the blood circulation occurred within 4-5 h. Even massive intravenous amounts of trypsin-alpha-macroglobulin complexes gave no demonstrable complement or kinin activation. Neither were other deleterious effects seen during the experiment or during 7 days of follow-up. The results indicate that treatment of acute pancreatitis in humans with alpha 2-macroglobulin might be possible, without any danger arising from the protease-alpha 2-macroglobulin complexes formed.
D000208:Acute Disease; D000818:Animals; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D007106:Immune Sera / Q000008:administration & dosage; D007262:Infusions, Intravenous; D010195:Pancreatitis / Q000097:blood*; D013552:Swine; D014238:Trichloroacetic Acid / Q000008:administration & dosage; D014357:Trypsin / Q000008:administration & dosage* / Q000097:blood*; D000511:alpha-Macroglobulins / Q000097:blood*
D007106:Immune Sera; C028939:alpha-2-macroglobulin-trypsin complex; D000511:alpha-Macroglobulins; D014238:Trichloroacetic Acid; D014357:Trypsin