Direct measurement of inositol in bovine myelin basic protein.
Myelin basic protein has been isolated from bovine central-nervous-system myelin by four methods, none of which exposes the protein to acid. After purification the inositol content of both hydrolysed and unhydrolysed protein was quantified by g.c.-m.s. Basic protein prepared by all methods contained less than 4 mol % of inositol. It is concluded, contrary to a previous proposal, that covalent binding to phosphoinositides does not represent a general mechanism for attachment of this cytoplasmically-oriented protein to its membrane.
D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D008401:Gas Chromatography-Mass Spectrometry; D007294:Inositol / Q000032:analysis*; D008722:Methods; D004676:Myelin Basic Protein
D004676:Myelin Basic Protein; D007294:Inositol