Analysis of the incompatibility determinants of I-complex plasmids.
The isolation and characterization of minireplicons corresponding to group B and I-complex plasmids are reported. The molecular structures of the small RNAs that may play a major role in the replication control and incompatibility reactions of the plasmids are compared. A mutant plasmid with changed copy number and incompatibility specificity is described. This mutant had a single-base-pair substitution in the DNA region that codes for the small RNA.
D001483:Base Sequence; D002874:Chromosome Mapping; D004252:DNA Mutational Analysis; D004261:DNA Replication; D004251:DNA Transposable Elements; D004720:Endonucleases; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D006860:Hydrogen Bonding; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010957:Plasmids; D012329:RNA, Bacterial / Q000235:genetics*; D012093:Replicon; D015719:Single-Strand Specific DNA and RNA Endonucleases
D004251:DNA Transposable Elements; D012329:RNA, Bacterial; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases