A procedure for Northern blot analysis of native RNA.
We describe a modification of the Northern technique that allows the detection of RNA either native and/or containing hidden breaks. We found that the highest sensitivity of the hybridization signals was obtained after denaturation of the RNA in the gel prior to its transfer onto a nylon membrane (GeneScreen) followed by uv irradiation. The sensitivity of the method using native RNA was found to be equivalent to that obtained with denatured RNA.
D000818:Animals; D001345:Autoradiography; D001358:Avian Sarcoma Viruses / Q000032:analysis; D002642:Chick Embryo; D004587:Electrophoresis, Agar Gel; D051379:Mice; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis
D012367:RNA, Viral; D012313:RNA