Lipids as integral constituents of nuclear and chromatin fractions in Ehrlich ascites tumor cells.
Lipid content and composition of DNA, histone and non-histone proteins of Ehrlich ascites tumor cell chromatin were investigated. All fractions contained small amounts of lipids, mostly neutral ones, in a specific distribution. According to isotopic studies with labeled lipid precursors, incorporation took place mainly in the non-histone fraction. These findings suggest that neutral lipids attached to non-histone chromosomal proteins may also contribute to the regulatory functions ascribed to phospholipids.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis*; D002467:Cell Nucleus / Q000032:analysis*; D002784:Cholesterol / Q000032:analysis / Q000378:metabolism; D002843:Chromatin / Q000032:analysis* / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis; D004247:DNA / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005990:Glycerol / Q000378:metabolism; D006657:Histones / Q000032:analysis; D008055:Lipids / Q000032:analysis*; D008297:Male; D051379:Mice; D010743:Phospholipids / Q000032:analysis; D012313:RNA / Q000032:analysis
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D008055:Lipids; D010743:Phospholipids; D012313:RNA; D004247:DNA; D002784:Cholesterol; D005990:Glycerol