title: Matrix-driven translocation: dependence on interaction of amino-terminal domain of fibronectin with heparin-like surface components of cells or particles.
abstract: During the process of matrix-driven translocation, certain types of cells or polystyrene latex beads are transported between compositionally different regions of a collagen matrix. Under appropriate conditions this translocation depends on an interaction between the cell or particle surface and fibronectin. We now show that this interaction takes place at a site located within the first 31 kDa of the amino-terminal end of the fibronectin molecule. Using defined fibronectin fragments and monoclonal antibodies directed against specific fibronectin domains, this site is established as both necessary and sufficient for the promotion of matrix-driven translocation. Competition experiments using heparin, heparan sulfate, and other sulfated polysaccharides show that this fibronectin site interacts with heparin-like cell or particle surface components in promoting matrix-driven translocation. Treatment of cells with heparinase renders them unresponsive to the translocational effect. An antibody directed against the amino-terminal domain of fibronectin completely inhibits matrix-driven translocation without interfering with heparin binding, suggesting that a post-binding conformational change in fibronectin may be required for promotion of the effect.
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D001665:Binding Sites; D055592:Biophysical Phenomena; D001703:Biophysics; D003094:Collagen; D016264:Dextran Sulfate; D003911:Dextrans / Q000378:metabolism; D000939:Epitopes / Q000276:immunology; D005353:Fibronectins / Q000276:immunology / Q000378:metabolism*; D005782:Gels; D006493:Heparin / Q000378:metabolism*; D006497:Heparan Sulfate / Q000378:metabolism; D006801:Humans; D007840:Latex; D008863:Microspheres; D009038:Motion; D010446:Peptide Fragments / Q000276:immunology; D011485:Protein Binding; D013499:Surface Properties
chemical_list: D000911:Antibodies, Monoclonal; D003094:Collagen; D016264:Dextran Sulfate; D003911:Dextrans; D000939:Epitopes; D005353:Fibronectins; D005782:Gels; D006493:Heparin; D006497:Heparan Sulfate; D007840:Latex; D010446:Peptide Fragments