title: [Electron microscopic study of preleptotene-stage oocyte nuclei in the prophase of meiosis in the hen].
abstract: The morphology of oocyte nuclei at preleptotene and early leptotene stages of meiotic prophase I in the chick embryo was examined by electron microscopy and light cytochemistry. The intrenuclear fibrillar body (IFB) of proteinaceous nature is described. The IFB has a spherical or irregular form and consists of the disoriented fibrils ranging from 3 to 18 nm in diameter and of globules lying along the course of fibrils. The condensed chromatin in the oocyte nuclei at the early--late preleptotene stages and the thread-like chromosomes in the oocyte nuclei at the middle preleptotene--early leptotene stages either closely adjoin to IFB, localized in the centre of the nucleus, or are situated at some distance from it, and then the fibrils or fibrillar filaments are seen between chromosome material and IFB. The chromosomes are attached to IFV by the telomer or interstitial segment. The chromosomes lose the connection with IFB after the attachment of both the telomeres to the nuclear envelope (middle--late leptotene), and IFB removes from the centre of the nucleus to its periphery. It is supposed that IFB represents a nuclear skeleton element and takes part in the spatial organization of the chromosomes in the oocyte nuclei at the early stages of meiotic prophase I.
mesh_terms: D000818:Animals; D001345:Autoradiography; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure*; D002642:Chick Embryo; D004247:DNA / Q000378:metabolism; D005260:Female; D006651:Histocytochemistry; D008540:Meiosis; D008854:Microscopy, Electron; D009865:Oocytes / Q000378:metabolism / Q000648:ultrastructure*; D011418:Prophase; D012313:RNA / Q000378:metabolism
chemical_list: D012313:RNA; D004247:DNA
vernacular_title: Elektronno-mikroskopicheskoe issledovanie iader ootsitov preleptotennoĭ stadii profazy meĭoza u kuritsy.