title: A nuclear deoxyribonucleic acid analysis of normal and abnormal vulvar epithelium.
abstract: Vulvar and groin skin from 21 women with clinical squamous cell carcinoma of the vulva was compared with that from nine patients with vulvar intraepithelial neoplasia. Feulgen-stained nuclear densitometry showed a constancy of Feulgen-deoxyribonucleic acid (DNA) density in normal epithelium and all subepithelial connective tissue nuclei. At three sites--tumor edge, opposite labia, and perineum--there were significant differences in nuclear chromatin content between the two groups and also as compared with normal epithelium.
mesh_terms: D001706:Biopsy; D002294:Carcinoma, Squamous Cell / Q000032:analysis; D002467:Cell Nucleus / Q000032:analysis*; D004396:Coloring Agents; D004247:DNA / Q000032:analysis*; D004848:Epithelium / Q000032:analysis / Q000473:pathology; D005260:Female; D006801:Humans; D012394:Rosaniline Dyes; D013194:Staining and Labeling / Q000379:methods; D014844:Vulva / Q000032:analysis* / Q000473:pathology; D014846:Vulvar Neoplasms / Q000032:analysis
chemical_list: D004396:Coloring Agents; C007722:Feulgen stain; D012394:Rosaniline Dyes; D004247:DNA