[Characteristics of antigen-antibody interaction in the reversed micelles of surface-active agents in heptane].
The alterations in the catalytic activity of the horseradish peroxidase after its interaction with antibodies against this enzyme have been studied in buffered solution and in reversed Aerosol OT (AOT) micelles in heptane. The antibodies were obtained by immunizing the rabbits with electrophoretically homogeneous enzyme and were purified by affinity chromatography. In the AOT micelles and mixed micelles containing AOT and Triton X-45, the enzyme interacted with antibodies very rapidly (in less than 5 min), i.e. the micelles did not hinder effective interaction between the enzyme and antibodies. The decrease in the peroxidase catalytic activity upon its interaction with antibodies in a micellar medium was determined by [H2O]/[AOT] ratio, pH and molarity of polar nucleus, as well as by the initial concentration of antibody. In buffered solutions, the decrease n the peroxidase activity of the enzyme--antibody complex was only weakly dependent on pH and molarity of a buffer solution.
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000937:Antigen-Antibody Reactions; D001667:Binding, Competitive; D004143:Dioctyl Sulfosuccinic Acid; D006536:Heptanes; D006735:Horseradish Peroxidase / Q000032:analysis / Q000276:immunology; D066298:In Vitro Techniques; D007700:Kinetics; D008823:Micelles; D011817:Rabbits; D013379:Substrate Specificity; D013501:Surface-Active Agents
D000906:Antibodies; D006536:Heptanes; D008823:Micelles; D013501:Surface-Active Agents; D004143:Dioctyl Sulfosuccinic Acid; D006735:Horseradish Peroxidase
Osobennosti vzaimodeĭstviia "antigen-antitelo" v obrashchennykh mitsellakh poverkhnostno-aktivnykh veshchestv v geptane.