[Cation transport and content in serum-stimulated CHO-773 cells. III. The intracellular content of sodium, calcium and magnesium].
Intracellular sodium, calcium, and magnesium content as well as lithium influx have been examined in serum-stimulated CHO cultures using flame-emission technique. Intracellular sodium and lithium influx does not change during the G1----S transition, they increase by 1.3-2 times in the late S and in mitosis. In stationary cultures of CHO cells cellular magnesium is about 50-60 mumole/gr protein; its content increases in 2-3 hours after serum addition and remains constant during the G1----S transition. In stationary cultures of CHO cells cellular calcium is about 20 mumole/gr protein and it increases by 1.5-2 times in the late G1 and S phases. It is concluded that alterations of ion transport accompany not only the early cell response to mitogen but also the G1----S transition.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology*; D007399:Interphase / Q000187:drug effects; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism; D008094:Lithium / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D013053:Spectrophotometry
D003470:Culture Media; D007473:Ion Channels; D008094:Lithium; D012964:Sodium; D008274:Magnesium; D002118:Calcium
Transport i soderzhanie kationov v kletkakh CHO-773, stimulirovannykh syvorotkoĭ. III. Vnutrikletochnoe soderzhanie natriia, kal'tsiia i magniia.