Recombinant gamma interferon induces HLA-DR expression on squamous cell carcinoma, trichilemmoma, adenocarcinoma cell lines, and cultured human keratinocytes.
We investigated the effects of recombinant human gamma interferon on the induction of HLA-DR expression by two human squamous cell carcinoma, three trichilemmoma, one eccrine carcinoma, two adenocarcinoma cell lines, and cultured human keratinocytes in vitro. None of eight epithelial cell lines or keratinocytes expressed HLA-DR without gamma interferon treatment. In contrast, pure gamma interferon (500 IU/ml, 72-h treatment) induced HLA-DR expression on 1/2 squamous cell carcinoma, 3/3 trichilemmoma, 2/2 adenocarcinoma cell lines, and 4/4 keratinocyte cell lines, as determined using a fluorescence-activated cell sorter. A maxillary squamous cell carcinoma line and an eccrine carcinoma cell line failed to express HLA-DR with gamma interferon treatment; however, the growth of cells was inhibited by gamma interferon treatment. By indirect immunoperoxidase techniques, tumor cells such as Bowen's disease and squamous cell carcinoma were found to express HLA-DR. Since HLA-DR expression has been shown to be important for various immune responses, these findings suggest that gamma interferon plays important roles in various immune-related skin diseases.
D000230:Adenocarcinoma / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000276:immunology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004817:Epidermis / Q000276:immunology* / Q000473:pathology; D006681:HLA-D Antigens / Q000276:immunology*; D006684:HLA-DR Antigens / Q000276:immunology*; D006197:Hair; D006651:Histocytochemistry; D006801:Humans; D007120:Immunochemistry; D007371:Interferon-gamma / Q000494:pharmacology*; D007633:Keratins; D011994:Recombinant Proteins / Q000494:pharmacology*; D012878:Skin Neoplasms / Q000276:immunology*
D006681:HLA-D Antigens; D006684:HLA-DR Antigens; D011994:Recombinant Proteins; D007633:Keratins; D007371:Interferon-gamma