title: A germ line specific DNA sequence is transcribed in Tetrahymena.
abstract: In the ciliated protozoan Tetrahymena 10-20% of the DNA sequences are micronucleus (germ line) specific. Six members of a family of repeated mic-specific DNA sequences are homologous to a 1.5-kb poly(A)+ RNA. The transcript is present in mature cells starved in 10 or 60 mM Tris, in starved immature cells, and in stationary cells. RNA from log-phase and heat-shocked cells does not have detectable levels of the transcript. These data indicate that at least one germ line limited DNA sequence is transcribed in the micronucleus of Tetrahymena.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D005854:Germ Cells / Q000648:ultrastructure; D006358:Hot Temperature; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000235:genetics; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D013768:Tetrahymena / Q000235:genetics* / Q000254:growth & development; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA; D004262:DNA Restriction Enzymes