The 5' flanking region of the human pS2 gene mediates its transcriptional activation by estrogen in MCF-7 cells.
The pS2 gene is transcriptionally induced by oestrogen in the human breast cancer cell line MCF-7. We demonstrate here that the 5' flanking sequences (-3000 to +10 bp) of the pS2 gene possess the properties of an oestrogen-inducible promoter. Interestingly, this oestrogen induction could not be demonstrated in transient transfection assays in MCF-7 cells, but only in stably transformed MCF-7 cells, which suggests that some factors responsible for oestrogen induction may be present in limiting amounts in these cells and absent in HeLa cells.
D001483:Base Sequence; D001943:Breast Neoplasms; D002285:Carcinoma, Intraductal, Noninfiltrating; D004720:Endonucleases; D004967:Estrogens / Q000494:pharmacology*; D005786:Gene Expression Regulation; D005796:Genes; D006367:HeLa Cells; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics*; D010957:Plasmids; D011506:Proteins; D012313:RNA / Q000032:analysis; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014158:Transcription, Genetic / Q000187:drug effects*; D014162:Transfection; D000071163:Trefoil Factor-1; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins
D004967:Estrogens; D009363:Neoplasm Proteins; D011506:Proteins; C051838:TFF1 protein, human; D000071163:Trefoil Factor-1; D025521:Tumor Suppressor Proteins; D012313:RNA; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases