Purified trichophytin: lymphocyte reactivity in vitro and trichophytin-induced suppression.
Three trichophytin preparations from different strains of Trichophyton mentagrophytes were produced according to the ethylene glycol method and assessed for their lymphocyte stimulation activity in vitro (LST). These trichophytin preparations showed significant variations in their effects on cord lymphocytes reactivity. The preparations also varied in their stimulation of lymphocytes from patients with dermatophytosis. Conclusions could be drawn about the immunological specificity, sensitivity and lymphocyte toxicity, which is important for the standardization of antigens. Furthermore, it was demonstrated that trichophytin-preincubated lymphocytes mediated a suppression of lymphocyte reactivity to this antigen.
D000328:Adult; D000946:Antigens, Fungal / Q000494:pharmacology*; D005312:Fetal Blood; D006801:Humans; D007231:Infant, Newborn; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects*; D014005:Tinea / Q000378:metabolism / Q000382:microbiology; D014248:Trichophytin / Q000302:isolation & purification / Q000494:pharmacology*; D014249:Trichophyton / Q000032:analysis / Q000235:genetics
D000946:Antigens, Fungal; D014248:Trichophytin