[Cytochemical research on the state of the histone component and of chromatin matrix activity in neurocytes of the rat sympathetic ganglion at different times after dissection of the pre- and postganglionic nerves].
Chromatin template activity and histones were studied in the rat superior cervical ganglion neurons of the 2nd, 10th and 60th days after bilateral pre- or postganglionic nerve division using the Moore method and the Black and Ansley ammoniacal silver staining technique. Alterations in chromatin template activity were found on the 2nd, and 10th days after operation when specific function of neurons was switched off. The dynamics of template activity was different for nucleolar and nucleoplasmic chromatins to be dependent on the type of operation. At the same periods after pre- and postganglionic nerve section the pattern of ammoniacal silver staining in neuron nuclei in the superior cervical ganglion became similar to that in nondifferentiated neurons. These alterations in transcription and histones disappeared by the 60th day after both the variants of operation. Changes in histone staining were absent after the false operation without nerve division. A conclusion is made on the existing of some connection between histone transformation during differentiation and specific functioning of nerve cells.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D005728:Ganglia, Sympathetic / Q000378:metabolism*; D006651:Histocytochemistry; D006657:Histones / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D009562:Nissl Bodies / Q000378:metabolism*; D051381:Rats; D013194:Staining and Labeling / Q000379:methods; D013562:Sympathectomy; D013997:Time Factors; D014158:Transcription, Genetic
D006657:Histones
Tsitokhimicheskoe issledovanie sostoianiia gistonovogo komponenta i matrichnoĭ aktivnosti khromatina v nevrotsitakh simpaticheskogo gangliia krysy na raznykh srokakh posle pererezki pre- i postganglionarov.