Changing intermediate-sized filament patterns in metastatic hepatocellular carcinoma cells of the guinea pig.
Hepatocellular carcinoma cells obtained from ascitic fluid after diethylnitrosamine treatment of Sewall Wright strain-2 guinea pigs produce solid (primary) tumors, lymph-node metastases and malignant ascites when reinjected into animals of the same strain. When brought into culture the cells settle, form multilayer cultures and can be maintained in passage. In addition to epithelium-specific cytokeratin intermediate filaments (IF), these latter cells, like most cultured cells, also contain vimentin. Hepatocellular carcinoma cells in solid tumors and in metastatic tumors retain their original keratin IF and in general do not have an additional vimentin-IF system. When the tumor cells are present in ascites they develop vimentin-IF in addition to cytokeratin filaments. Vimentin is gradually lost when these cells sediment onto the peritoneal surface and proliferate continuously to form papillary projections, or when they are detected as circumscribed metastases. It seems likely, therefore, that in this system the synthesis of an additional vimentin cytoskeleton is related to reduced cell-to-cell contact and to the ability of the cells to survive individually or as cell clusters in body fluids, without being part of a cohesive tissue.
D000818:Animals; D001201:Ascites / Q000473:pathology; D003599:Cytoskeleton / Q000648:ultrastructure*; D003893:Desmin / Q000378:metabolism; D003896:Desmosomes / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D007382:Intermediate Filaments / Q000648:ultrastructure*; D007633:Keratins / Q000378:metabolism; D008099:Liver / Q000648:ultrastructure; D008114:Liver Neoplasms, Experimental / Q000648:ultrastructure*; D008207:Lymphatic Metastasis; D014746:Vimentin / Q000378:metabolism
D003893:Desmin; D014746:Vimentin; D007633:Keratins