title: The effect of a new intraocular irrigating solution containing dextran on rabbit corneal endothelium.
abstract: This study investigated the effect of a balanced salt solution for intraocular irrigation containing 2% dextran 40. The effect of this solution was compared to that of a normal saline solution (0.9%) and balanced salt solution (Alcon). Following incubation of rabbit corneas for up to 72 h at 4 degrees C, the incubated corneas were studied by staining with Nitroblue Tetrazolium and by scanning electron microscopy. Insignificant changes occurred during the first 24 h of incubation. However, corneas stored in the dextran containing solution showed slightly smaller endothelial cells with tight junctions after 72 h. Endothelial cells showed progressive membrane rupture and eventual disappearance when incubated in normal saline for over 24 h at 4 degrees C.
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D003315:Cornea / Q000187:drug effects* / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D003911:Dextrans / Q000494:pharmacology*; D004338:Drug Combinations / Q000494:pharmacology; D004727:Endothelium / Q000187:drug effects / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D009580:Nitroblue Tetrazolium; D009883:Ophthalmic Solutions; D011817:Rabbits; D012965:Sodium Chloride / Q000494:pharmacology*; D013194:Staining and Labeling; D007507:Therapeutic Irrigation; D014617:Vacuoles / Q000648:ultrastructure
chemical_list: D003911:Dextrans; D004338:Drug Combinations; D009883:Ophthalmic Solutions; C049389:dextran - saline drug combination; D009580:Nitroblue Tetrazolium; D012965:Sodium Chloride