title: Amylase expression in human parotid neoplasms: evidence by in situ hybridization for lack of transcription of the amylase gene.
abstract: Salivary alpha-amylase (EC 3.2.1.1) is the major protein component of human parotid gland secretion. We studied amylase gene structure and expression in tissue from a series of normal and neoplastic parotid glands by Southern blot analysis, in situ hybridization, and immunohistochemistry. Thirty-two tumors were examined. Southern blot analysis of DNA extracted from a Warthin tumor, an adenoid cystic carcinoma, and a mucoepidermoid carcinoma showed no evidence of structural rearrangement of amylase genes. Eleven parotid Warthin tumors were negative for amylase protein and mRNA by immunocytochemistry and in situ hybridization. One pleomorphic adenoma in the group of 10 examined showed focal staining for amylase protein, although amylase mRNA could not be demonstrated in the same population of cells by in situ hybridization in serial tissue sections. Five mucoepidermoid carcinomas and three acinar cell carcinomas were devoid of amylase protein and mRNA. Normal parotid tissue obtained from all patients studied revealed abundant acinar cell amylase mRNA and protein. In situ hybridization, in conjunction with immunocytochemistry, allows precise cellular localization of mRNA and protein, thereby establishing the site of production of specific transcripts. We conclude that the interruption in amylase gene expression in parotid gland neoplasms occurs at the transcriptional level.
mesh_terms: D000681:Amylases / Q000032:analysis / Q000235:genetics*; D004247:DNA / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D009693:Nucleic Acid Hybridization; D010307:Parotid Neoplasms / Q000201:enzymology*; D012333:RNA, Messenger / Q000032:analysis; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D004247:DNA; D000681:Amylases