Photodamage to the lens in vitro: implications of the Haber-Weiss reaction.
Studies have been conducted to examine the implications of photochemical generation of O2- and its derivatization to H2O2 and OH . in the physiology of the lens in vitro. Physiological status was determined by measuring the uptake of rubidium by the intact tissue when cultured in riboflavin-containing medium, in dark and light, and in the presence and absence of various scavengers. In the presence of light, the uptake of rubidium in the lens was greatly diminished; this suggests photodamage to the tissue. MnSOD and ferricyanide protected against this photochemical damage. The damaging process was thus initiated by the generation of O2-. The tissue damage was also attenuated by catalase, ferrocyanide, and mannitol. These results, therefore, suggest the participation of hydrogen peroxide and the subsequent Haber-Weiss reaction in the photodamaging process.
D000818:Animals; D002386:Cataract / Q000209:etiology; D006861:Hydrogen Peroxide / Q000378:metabolism; D066298:In Vitro Techniques; D007473:Ion Channels / Q000378:metabolism; D007908:Lens, Crystalline / Q000378:metabolism / Q000528:radiation effects*; D008027:Light / Q000009:adverse effects*; D010777:Photochemistry; D011188:Potassium / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism*
D007473:Ion Channels; D013481:Superoxides; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; D011188:Potassium