title: Structural basis for altered soybean agglutinin lectin binding between a murine metastatic lymphoma and an adhesive low malignant variant.
abstract: By selection for plastic adhesiveness we have previously established a variant tumor line (ESb-MP) from the metastatic murine lymphoma ESb. In contrast to the parental line, the adhesion variant is significantly decreased in malignancy and is altered in the capacity to bind soybean agglutinin (SBA) lectin. Here we show biochemically that the major SBA-binding cell-surface component of ESb-MP cells is the T200 glycoprotein. In ESb cells, T200 antigens bind SBA only after sialidase treatment. Enzymatic studies suggested that glycans detected by the lectin with or without sialidase treatment are different. Inhibition of N-glycosylation by tunicamycin and biosynthetic labeling revealed two T200 chains for ESb-MP cells that were larger in size than the single chain detected in ESb cells. Studies on the biosynthesis revealed that ESb-MP cells expressed two precursor chains for T200 whereas ESb cells displayed only one. There was no size difference detectable in the mature T200 molecules of ESb and ESb-MP cells. Our data suggest that the molecules differ in expression of O-linked glycans that can be recognized by SBA. Additional O-linked sugars on ESb-MP T200 molecules seem to be expressed in particular after trimming of the second T200 precursor chain.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002240:Carbohydrate Sequence; D002448:Cell Adhesion; D000939:Epitopes; D005690:Galactose / Q000502:physiology; D006023:Glycoproteins / Q000378:metabolism*; D007526:Isoelectric Point; D037102:Lectins / Q000378:metabolism*; D008223:Lymphoma / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008970:Molecular Weight; D009362:Neoplasm Metastasis; D037121:Plant Lectins; D010969:Plastics; D012794:Sialic Acids / Q000502:physiology; D030262:Soybean Proteins; D013329:Structure-Activity Relationship; D010835:Phytohemagglutinins
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D005690:Galactose; D006023:Glycoproteins; D037102:Lectins; D037121:Plant Lectins; D010969:Plastics; D012794:Sialic Acids; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins