[A simple test for quantitative determination of LDL-cholesterol].
The subject of the report is a novel precipitation test for the quantitative recording of LDL cholesterol based on the precipitation of LDL by dextran sulphate. Parallel assays of LDL cholesterol according to the new method and using quantitative lipoprotein electrophoresis as reference showed the results, in terms of the individual values and collectively, to be practically identical for a wide concentration range of various lipids and lipoproteins in the serum. The concentration ratio of the means obtained according to the two methods is 1.014 +/- 0.102 (standard deviation). The regression function displays a correlation coefficient of 0.9470. Double assays with the new technique yield a variation coefficient of 1.7 +/- 0.4%. Limitations of the method, which are insignificant for application in practice, are pointed out. The new precipitation method is simple, safe and useful for the quantitative estimation of the LDL cholesterol concentration in freshly obtained human serum. The method requires only little time and equipment.
D008078:Cholesterol, LDL / Q000097:blood*; D016264:Dextran Sulfate; D003911:Dextrans; D004586:Electrophoresis; D006801:Humans
D008078:Cholesterol, LDL; D003911:Dextrans; D016264:Dextran Sulfate
Einfaches Verfahren zur quantitativen Bestimmung von LDL-Cholesterin.