title: On the use of anti-sense RNA: down-regulation of mRNA encoding a metalloproteinase inhibitor.
abstract: In this article we describe methods used for producing anti-sense RNA and the mechanisms by which specific eukaryotic gene functions are inhibited. We also describe some of our studies with plasmid constructs producing anti-sense RNA targeted to a murine metalloproteinase inhibitor and review the evidence for naturally occuring anti-sense transcripts in eukaryotes.
mesh_terms: D000818:Animals; D004791:Enzyme Inhibitors / Q000235:genetics*; D005786:Gene Expression Regulation; D012313:RNA / Q000235:genetics*; D016372:RNA, Antisense; D019714:Tissue Inhibitor of Metalloproteinases
chemical_list: D004791:Enzyme Inhibitors; D016372:RNA, Antisense; D019714:Tissue Inhibitor of Metalloproteinases; D012313:RNA