Dye affinity chromatography of ricin subunits.
A rapid and simple method for the isolation of the two chains of ricin is described. The method involves two chromatographic runs, the first on blue-Sepharose and the second on blue dextran-Sepharose. It is moreover shown that the A chain of ricin, but not the B chain, binds to poly(U)-Sepharose.
D002846:Chromatography, Affinity / Q000379:methods; D003911:Dextrans / Q000378:metabolism; D046911:Macromolecular Substances; D011072:Poly U / Q000378:metabolism; D012276:Ricin / Q000302:isolation & purification*; D012685:Sepharose / Q000031:analogs & derivatives / Q000378:metabolism
D003911:Dextrans; D046911:Macromolecular Substances; D011072:Poly U; C029483:Blue-Sepharose; D012276:Ricin; D012685:Sepharose; C017080:blue dextran