title: Activities of the RNAI and RNAII promoters of plasmid pBR322.
abstract: The synthesis rates of the replication control RNAs of plasmid pBR322, RNAI, an inhibitor of replication, and RNAII, the preprimer, have been determined by hybridizing in vivo pulse-labeled RNA to specific, single-stranded DNA probes for RNAI and RNAII. In Escherichia coli growing in glycerol minimal medium, RNAI transcripts were made at a rate of one molecule per 30 s per plasmid; RNAII was transcribed fivefold less, at a rate of one molecule per 3 min per plasmid. It is estimated that only 1 in 20 prepriming events leads to replication.
mesh_terms: D003090:Coliphages / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012313:RNA / Q000235:genetics*; D012329:RNA, Bacterial / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: C042027:RNA I; C034477:RNA primers; D012329:RNA, Bacterial; D012313:RNA