Volatile anesthetics depress the depolarization-induced cytoplasmic calcium rise in PC 12 cells.
In the rat pheochromocytoma cell line PC 12, the effects of four volatile anesthetics (halothane, isoflurane, enflurane, methoxyflurane) on the K+-evoked intracellular calcium [( Ca2+]i) rise were investigated using the Ca2+-sensitive fluorescence dye fura-2. The [Ca2+]i rise was depressed, at clinical concentrations, by all anesthetics with almost identical aqueous IC50 values. The study extends to neuronal cells the observation made previously in cardiac tissue that volatile anesthetics may interfere with Ca2+ fluxes through voltage-gated channels.
D000310:Adrenal Gland Neoplasms / Q000378:metabolism*; D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D001572:Benzofurans; D002460:Cell Line; D003593:Cytoplasm / Q000378:metabolism*; D004737:Enflurane / Q000494:pharmacology; D005456:Fluorescent Dyes; D016257:Fura-2; D006221:Halothane / Q000494:pharmacology; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism; D007530:Isoflurane / Q000494:pharmacology; D008733:Methoxyflurane / Q000494:pharmacology; D010673:Pheochromocytoma / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D051381:Rats
D000777:Anesthetics; D001572:Benzofurans; D005456:Fluorescent Dyes; D007473:Ion Channels; D008733:Methoxyflurane; D004737:Enflurane; D007530:Isoflurane; D011188:Potassium; D016257:Fura-2; D006221:Halothane