[Value of rapid diagnosis of Plasmodium falciparum using indirect monoclonal immunofluorescence].
Development and use of murine specific monoclonal antibodies associated with an indirect immunofluorescent assay allows a rapid detection of P. falciparum antigens. This work compares the results obtained by interpretation of Giemsa staining technique on blood smears, to the use of a commercial reagent (Monofluo Kit P. falciparum). This monoclonal indirect immunofluorescent assay (I.F.I.M.) provides the clinician with a precious aid to diagnosis as well by its specificity and sensitivity, better than conventional staining techniques; particularly in pauciparasitaemia induced by prime-infestation or partial anti-malarial drug resistance.
D000818:Animals; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008288:Malaria / Q000469:parasitology*; D010963:Plasmodium falciparum / Q000276:immunology*; D011933:Reagent Kits, Diagnostic; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D011933:Reagent Kits, Diagnostic
Intêret du diagnostic rapide de Plasmodium falciparum par immunofluorescence indirecte monoclonale.