Tyrosine kinases in normal human blood cells. Platelet but not erythrocyte band 3 tyrosine kinase is p60c-src.
The major tyrosine kinase from platelets was purified as a 51 kDa active enzyme which was shown to be a degradation product of the protooncogene product p60c-src. Immuno-depletion experiments using a monoclonal antibody recognizing p60c-src failed to remove band 3 phosphorylating activity from red blood cell membranes. The erythrocyte tyrosine kinase was not at all immunoprecipitated by this antibody under conditions where the platelet enzyme was immunoprecipitated.
D001457:Anion Exchange Protein 1, Erythrocyte / Q000032:analysis; D001792:Blood Platelets / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D006868:Hydrolysis; D007120:Immunochemistry; D011505:Protein-Tyrosine Kinases / Q000097:blood*; D011518:Proto-Oncogene Proteins / Q000097:blood*; D016392:Proto-Oncogene Proteins pp60(c-src)
D001457:Anion Exchange Protein 1, Erythrocyte; D011518:Proto-Oncogene Proteins; D011505:Protein-Tyrosine Kinases; D016392:Proto-Oncogene Proteins pp60(c-src)