Change in the activity of the cyclic AMP-dependent protein kinase in antigen-stimulated sensitized mast cells and effect of drugs inhibiting allergic mediator release.
The activity of cyclic AMP-dependent protein kinase (protein kinase A) in the sensitized rat mast cell was decreased 2 min after antigen challenge when the histamine release exhibited a maximum. Drugs inhibiting allergic mediator release such as disodium cromoglycate, tranilast and theophylline significantly inhibited antigen-induced histamine release and reduced a decrease in the activity of protein kinase A. These results suggest that protein kinase A is involved in the histamine releasing process in the mast cell, and drugs inhibiting allergic mediator release cause their effects partially through the inhibition of protein kinase A.
D000818:Animals; D000941:Antigens / Q000276:immunology*; D004205:Cromolyn Sodium / Q000494:pharmacology; D006636:Histamine Release; D066298:In Vitro Techniques; D008297:Male; D008407:Mast Cells / Q000276:immunology / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013806:Theophylline / Q000494:pharmacology; D062367:ortho-Aminobenzoates / Q000494:pharmacology
D000941:Antigens; D062367:ortho-Aminobenzoates; D013806:Theophylline; D011494:Protein Kinases; C012293:tranilast; D004205:Cromolyn Sodium