Isotachophoretic and HPLC determination of nucleotides in rat liver cell nuclei isolated by non-aqueous technique.
Rat liver whole cells and cell nuclei were prepared by a non-aqueous technique (glycerol). The nuclear preparations were of different purity as determined by RNA/DNA ratios (0.17-1.60) and accordingly were divided into 3 subgroups (mean values 0.29, 1.04 and 1.48). RNA nucleotides were separated by isotachophoresis and HPLC and calculated per mg DNA. Two of the nuclear subgroups (RNA/DNA = 1.04 and 1.48) had significantly elevated nucleotide values in relation to RNA/DNA. UDP-N-acetylhexosamine/DNA, on the contrary, was reduced in conformity with RNA in the preparations. Our findings may indicate different nucleotide concentrations in different parts of the cell.
D000227:Adenine Nucleotides / Q000302:isolation & purification; D000818:Animals; D002467:Cell Nucleus / Q000032:analysis* / Q000648:ultrastructure; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004247:DNA / Q000302:isolation & purification*; D008099:Liver / Q000032:analysis*; D008297:Male; D008854:Microscopy, Electron; D012313:RNA / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains; D012265:Ribonucleotides / Q000302:isolation & purification*
D000227:Adenine Nucleotides; D012265:Ribonucleotides; D012313:RNA; D004247:DNA