Colloidal gold staining and immunoprobing of proteins on the same nitrocellulose blot.
Proteins blotted onto nitrocellulose can be stained with colloidal gold. They retain their immunoreactivity so that the stained blot can be probed with appropriate antibodies. The resulting antigen-antibody reaction is made visible by a peroxidase-coupled antispecies antibody. The blue peroxidase reaction product contrasts well with the red-stained protein pattern and allows an easy documentation by black-and-white photography.
D003101:Collodion; D006046:Gold; D007120:Immunochemistry; D007124:Immunoenzyme Techniques; D011506:Proteins / Q000032:analysis* / Q000276:immunology; D013194:Staining and Labeling
D011506:Proteins; D006046:Gold; D003101:Collodion