Induction of various HLA class II molecules in a human colonic adenocarcinoma cell line.
The colonic carcinoma cell line HT-29 had no constitutive expression of HLA class II molecules. Gamma interferon (IFN-gamma) induced expression of HLA class II molecules in a dose-dependent manner with 100 U/ml as an optimal dose. The expression of HLA-DR, HLA-DP, and HLA-DQ molecules seemed to follow different kinetics. While DR and DP molecules were maximally induced after 2 days, DQ molecules appeared later with maximum percentage positive cells after 8 days. Treatment with a prostaglandin synthesis inhibitor (indomethacin) neither induced class II expression nor altered the dose-response curve for IFN-gamma; this indicated that possible endogenous production of prostaglandins in this cell line did not interfere with its class II expression. The lectins phytohaemagglutinin (PHA), concanavalin A (Con A), pokeweed mitogen (PWM), and wheat germ agglutinin (WGA) did not induce class II expression.
D000230:Adenocarcinoma / Q000276:immunology / Q000473:pathology*; D002460:Cell Line; D003110:Colonic Neoplasms / Q000276:immunology / Q000473:pathology*; D000939:Epitopes / Q000502:physiology; D006681:HLA-D Antigens / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D007421:Intestine, Small / Q000276:immunology
D000939:Epitopes; D006681:HLA-D Antigens; D007371:Interferon-gamma