Yeast ribonuclease H(70) cleaves RNA-DNA junctions.
A specific substrate, M13 DNA:RNA-[32P]DNA, was synthesized to investigate the mode of cleavage of enzymes with RNase H activity. RNase H(70) from Saccharomyces cerevisiae hydrolyzes the phosphodiester bond at the RNA-DNA junction of this substrate, thereby producing a 5'-monophosphate-terminated polydeoxyribonucleotide and 3'-hydroxyl-terminated oligoribonucleotides.
D004247:DNA / Q000378:metabolism*; D004261:DNA Replication; D004722:Endoribonucleases / Q000378:metabolism*; D006868:Hydrolysis; D012313:RNA / Q000378:metabolism*; D016914:Ribonuclease H; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013379:Substrate Specificity
C034477:RNA primers; D012313:RNA; D004247:DNA; D004722:Endoribonucleases; D016914:Ribonuclease H