The effect of removal of D-fructose on the antigenicity of the lipopolysaccharide from a rough mutant of Vibrio cholerae Ogawa.
The lipopolysaccharide (LPS) of a rough mutant (95R) of Vibrio cholerae Ogawa has been investigated chemically and serologically. D-Fructose was released from LPS under conditions (10mM trifluoroacetic acid, 60 degrees, 1 h) that liberated no other sugar constituent of the LPS (2-amino-2-deoxy-D-glucose, D-glucose, L-glycero-D-manno-heptose). Upon periodate oxidation, D-fructose and D-glucose were oxidised quantitatively, whereas approximately 50% of heptose was periodate-resistant. The data indicate that D-fructose does not link the polysaccharide and lipid A portion as proposed earlier, and suggest that D-fructose is present as a branch. By passive hemolysis inhibition, it was shown that the release of D-fructose paralleled the exposure of an antigenic determinant cryptic in LPS.
D002241:Carbohydrates / Q000032:analysis; D000939:Epitopes / Q000032:analysis; D005632:Fructose; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000276:immunology*; D009154:Mutation; D010084:Oxidation-Reduction; D010504:Periodic Acid; D014734:Vibrio cholerae / Q000235:genetics / Q000276:immunology*
D002241:Carbohydrates; D000939:Epitopes; D008070:Lipopolysaccharides; D010504:Periodic Acid; D005632:Fructose