title: Surface phenotypic changes of spleen cells on acquisition of encephalitogenicity.
abstract: The relationship between surface marker expression and encephalitogenicity of spleen cells was studied in Lewis rats. Donors were sensitized with either BP/CFA or BP/IFA, or given BP-cultured naive spleen cells. The adoptive transfer of EAE was successfully achieved in every case after culture with BP, although only spleen cells from BP/CFA-sensitized rats proliferated significantly in response to BP. The observed encephalitogenicities were BP/CFA-sensitized cells greater than BP/IFA-sensitized cells much greater than cells from recipients of BP-cultured naive cells in descending order. In BP/CFA-sensitized cells, the expression of both W3/25 and OX-3 antigens on T cells increased markedly after culture with BP, but the expression of neither OX-19 nor OX-8 antigen increased significantly. Cells from neither BP/IFA-sensitized rats nor recipients of BP-cultured naive cells showed a significant change in the surface marker expression after culture with BP. Therefore, the generation of T cells coexpressing large amounts of both W3/25 and OX-3 antigens after culture with BP seems to correspond to the acquisition of the strong encephalitogenicity in vivo.
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology*; D005260:Female; D005620:Freund's Adjuvant; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D008213:Lymphocyte Activation; D004676:Myelin Basic Protein / Q000276:immunology; D010641:Phenotype; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000954:Antigens, Surface; D000949:Histocompatibility Antigens Class II; D004676:Myelin Basic Protein; D005620:Freund's Adjuvant