title: Similarities and dissimilarities in the structure and expression of viral genomes of various virus strains immunologically related to Marek's disease virus.
abstract: Various strains immunologically related to Marek's disease virus (MDV) have been subdivided into three serotypes: serotype 1, pathogenic strains of MDV and attenuated or apathogenic variants derived from them; serotype 2, naturally occurring apathogenic strains of MDV; serotype 3, herpesvirus of turkey (HVT). The viral genome structures of these three serotypes were compared by a simple, practical method using total DNA extracted from virus-infected cells instead of viral DNA purified from virions. The restriction endonuclease-cleavage patterns of serotype 2 viral DNA were found to differ from those of either serotype 1 MDV or serotype 3. Under stringent conditions, no significant DNA homology was detected among the three serotype viruses, except in a restricted portion of these viral genomes. Northern blot hybridization experiments suggested that virus-specific polyadenylated RNA of about 2.4 kilobases was transcribed from a restricted portion showing close homology in these viruses. Southern blot hybridization under less stringent conditions revealed that regions with weak homology were distributed over most of the viral genomes of the three serotypes. Two types of virus-specific glycoproteins, gA and gB, were identified in the immunoprecipitates of the culture medium and cell lysates, respectively, of serotype 2-infected cultures with monoclonal antibodies or hyperimmune antisera cross reactive with serotype 1 MDV and serotype 3 HVT, and detected on the surface of serotype 2-infected cells by the membrane immunofluorescence test. These results indicate a close evolutionary relationship among these three viral serotypes.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002642:Chick Embryo; D002645:Chickens; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D005347:Fibroblasts / Q000378:metabolism; D005814:Genes, Viral; D006564:Herpesviridae / Q000235:genetics*; D008381:Herpesvirus 2, Gallid / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000235:genetics*; D012313:RNA / Q000235:genetics*; D012333:RNA, Messenger; D012689:Sequence Homology, Nucleic Acid; D012703:Serotyping; D013045:Species Specificity; D014158:Transcription, Genetic; D014422:Turkeys; D014764:Viral Proteins / Q000235:genetics / Q000302:isolation & purification
chemical_list: D000911:Antibodies, Monoclonal; D004279:DNA, Viral; D012333:RNA, Messenger; D014764:Viral Proteins; D011061:Poly A; D012313:RNA; D004262:DNA Restriction Enzymes