A combined elastic, fibrin and collagen stain.
A method is described which demonstrates nuclei, elastic fibers, red blood cells, collagen and fibrin. Nuclei and elastic fibers are stained by a modified Verhoeff's elastic tissue stain which was previously developed and used in the elastic-Masson combination. Both early fibrin and red blood cells are shown by lissamine fast yellow. Mature fibrin, some types of collagen and other cytoplasmic changes are stained by a combination of acid fuchsin, Biebrich scarlet and ponceau 2R, while old fibrin is demonstrated by the collagen stain. This method takes about 1 hr to perform and has the added advantage that several entities are clearly shown in a single slide.
D002467:Cell Nucleus / Q000648:ultrastructure; D003094:Collagen / Q000032:analysis*; D004547:Elastic Tissue / Q000032:analysis*; D004912:Erythrocytes / Q000166:cytology; D005337:Fibrin / Q000032:analysis*; D006801:Humans; D009132:Muscles / Q000032:analysis; D013194:Staining and Labeling
D005337:Fibrin; D003094:Collagen