Nucleotide sequence of the pepN gene encoding aminopeptidase N of Escherichia coli.
We have sequenced a 3.3-kb fragment of the Escherichia coli chromosome that contains pepN gene encoding aminopeptidase N. This gene codes for a protein of 870 amino acid residues. From the size of the pepN transcript and the presence of inverted repeats in the nucleotide (nt) sequence, a putative transcription terminator has been identified. The N-terminal amino acid sequence deduced from the pepN nt sequence corresponds to the N-terminal sequence of the purified protein; the amino acid composition of the protein is also in good agreement with that deduced from the gene sequence. No obvious homology with previously sequenced peptidases has been detected.
D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000235:genetics*; D001483:Base Sequence; D018826:CD13 Antigens; D002874:Chromosome Mapping; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D011487:Protein Conformation; D012329:RNA, Bacterial / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics
D012329:RNA, Bacterial; D012333:RNA, Messenger; D000626:Aminopeptidases; D018826:CD13 Antigens