title: A combined Golgi and autoradiographic study of 3H-glycine-accumulating cone bipolar cells in the cat retina.
abstract: Morphological subclasses of cone bipolar cells in the cat retina were studied in Golgi preparations and through the combination of Golgi impregnation with quantitative electron-microscopic autoradiography of 3H-glycine accumulation. Camera lucida drawings, computer-assisted rotations, and radial sections provided the morphological details used in assigning individual cells to morphologically defined subclasses. Selected Golgi-impregnated cells from retinas preloaded with 3H-glycine were sectioned for autoradiographic analysis. Grain densities were quantitated and compared on a normalized scale with the labeling of amacrine cells with 3H-glycine. Eleven types of cone bipolar cells were identified, 6 with axon terminals ramifying in sublamina a and 5 with axons terminating in sublamina b. Of these cells, types CBb2 and CBb5 showed the highest affinity for glycine, with labeling at 41 and 38% with respect to the most heavily labeled amacrine cells. Types CBb1, CBb3, CBa2, and CBa6 were labeled at approximately 20% of maximal labeling. The accumulation of glycine by these cells suggests that they may be involved in providing inhibitory input to the inner retina.
mesh_terms: D000818:Animals; D001345:Autoradiography; D001369:Axons / Q000648:ultrastructure; D002415:Cats; D003712:Dendrites / Q000648:ultrastructure; D005998:Glycine / Q000378:metabolism*; D008854:Microscopy, Electron; D009474:Neurons / Q000648:ultrastructure*; D010781:Photography / Q000379:methods; D012160:Retina / Q000166:cytology* / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods
chemical_list: D005998:Glycine