title: Sites of action of two ribosomal RNA methylases responsible for resistance to aminoglycosides.
abstract: Methylation of either of two residues (G-1405 or A-1408) within bacterial 16 S ribosomal RNA results in high level resistance to specific combinations of aminoglycoside antibiotics. The product of a gene that originated in Micromonospora purpurea (an actinomycete that produces gentamicin) gives resistance to kanamycin plus gentamicin by converting residue G-1405 to 7-methylguanosine. Resistance to kanamycin plus apramycin results from conversion of residue A-1408 to 1-methyladenosine catalysed by the product of a gene from Streptomyces tenjimariensis.
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology*; D001483:Base Sequence; D001665:Binding Sites; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000201:enzymology; D008745:Methylation; D008780:Methyltransferases / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D012313:RNA; D012329:RNA, Bacterial / Q000378:metabolism; D012335:RNA, Ribosomal / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology
chemical_list: D000617:Aminoglycosides; C034477:RNA primers; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012313:RNA; D008780:Methyltransferases; C035181:rRNA (adenosine-O-2'-)methyltransferase