Synaptosomal sodium channels in rat lines selected for alcohol-related behaviors.
Alcohol has been shown to inhibit veratridine- and batrachotoxin-stimulated sodium flux in rodent brain synaptosomes. In this study, binding of [3H]batrachotoxinin A 20-alpha-benzoate ([3H]BTX-B) and uptake of [14C]guanidine in cerebrocortical synaptosomes were measured in naive rats belonging to lines with high or low acute sensitivity to ethanol (ANT, Alcohol Non-Tolerant, vs. AT, Alcohol Tolerant, lines) and to lines with high or low voluntary alcohol consumption (AA, Alko Alcohol, vs. ANA, Alko Non-Alcohol, lines). Our rat line pairs did not differ in the equilibrium binding characteristics of [3H]BTX-B nor in the properties of [14C]guanidine uptake, suggesting that the genetic selection has not modulated the genes or the expression of the genes associated with the voltage-sensitive sodium channels.
D000428:Alcohol Drinking / Q000502:physiology*; D000818:Animals; D001496:Batrachotoxins / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D019791:Guanidine; D006146:Guanidines / Q000493:pharmacokinetics; D066298:In Vitro Techniques; D007473:Ion Channels / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000378:metabolism*; D013045:Species Specificity; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*; D014701:Veratridine / Q000494:pharmacology
D001496:Batrachotoxins; D006146:Guanidines; D007473:Ion Channels; D014701:Veratridine; C031775:batrachotoxinin A 20-alpha-benzoate; D012964:Sodium; D019791:Guanidine