Fibrin glue coating of e-PTFE prostheses enhances seeding of human endothelial cells.
A laboratory study was undertaken to improve the initial count of endothelial cells (EC) adhering to the wall of e-PTFE prostheses when seeding of human EC is attempted. In our experiments pretreatment of the prosthetic wall with commercially available fibrin glue (Tissucol) improved the reliability of the seeding procedure. The number and the distribution of EC seeded onto fibrin glue presealed e-PTFE prostheses was compared to the number and distribution of EC adhering to blood preclotted grafts 24 hours following the initial seeding procedure. Fibrin glue presealed grafts showed a higher number of initially adhering EC and a more equal distribution over the graft surface when compared to blood pretreated grafts. Our results suggest that the use of fibrin glue enhances the seeding of human EC on e-PTFE grafts.
D000941:Antigens / Q000032:analysis; D007611:Aprotinin / Q000494:pharmacology*; D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion / Q000187:drug effects; D004338:Drug Combinations / Q000494:pharmacology; D004727:Endothelium / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D005169:Factor VIII / Q000032:analysis / Q000276:immunology; D005176:Factor XIII / Q000494:pharmacology*; D015718:Fibrin Tissue Adhesive; D005340:Fibrinogen / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D011138:Polytetrafluoroethylene; D013917:Thrombin / Q000494:pharmacology*; D014014:Tissue Adhesives / Q000494:pharmacology*; D014680:Veins / Q000166:cytology; D014841:von Willebrand Factor / Q000032:analysis
D000941:Antigens; D004338:Drug Combinations; D015718:Fibrin Tissue Adhesive; D014014:Tissue Adhesives; D014841:von Willebrand Factor; D005169:Factor VIII; D005340:Fibrinogen; D011138:Polytetrafluoroethylene; D005176:Factor XIII; D007611:Aprotinin; D013917:Thrombin