Iron pyronine and alcian blue for staining acid mucin in plastic-embedded sections.
Iron pyronine Y and Alcian blue demonstrated sulfated and nonsulfated acid mucin, respectively, in plastic-embedded sections. Safran used in combination with these two dyes stained collagen and some reticulum fibers. Sulfated acid mucin was red, while nonsulfated acid mucin stained blue; collagen appeared yellow to greenish yellow. The iron pyronine Y-Alcian blue-safran staining method, when used as in the present article, produces excellent cellular visualization of sulfated and nonsulfated acid mucin.
D000423:Alcian Blue; D002712:Chlorides; D005290:Ferric Compounds; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007211:Indoles; D007422:Intestines / Q000032:analysis; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis; D009077:Mucins / Q000032:analysis*; D011754:Pyronine; D013194:Staining and Labeling; D014966:Xanthenes
D002712:Chlorides; D005290:Ferric Compounds; D007211:Indoles; D009077:Mucins; D014966:Xanthenes; D000423:Alcian Blue; C024555:ferric chloride; D011754:Pyronine