A simple and rapid method for the isolation of human alpha-fetoprotein from human cord serum.
A description is given of a simple and time-saving method for the isolation of human alpha-fetoprotein (AFP) by means of liquid chromatography. The isolation was made in three steps: Step 1--affinity chromatography on column CNBr-Sepharose 4 B coupled with sheep antibody against human alpha-fetoprotein. AFP was eluted from the column by a change pH. Step 2--application of AFP to column Blue-Sepharose CL-6B for the removal of albumin remnants. Step 3--additional purification of the AFP preparation on column Con-A-Sepharose. AFP was eluted from the column by using methyl-alpha-D-glucepyranoside gradient. The isolation yield was 20%. The purity of the AFP preparation was satisfactory for RIA.
D002846:Chromatography, Affinity; D005312:Fetal Blood / Q000032:analysis*; D006801:Humans; D005779:Immunodiffusion; D007164:Immunosorbents; D000509:alpha-Fetoproteins / Q000302:isolation & purification*
D007164:Immunosorbents; D000509:alpha-Fetoproteins