Differentiation in vitro of primary cultures and transfected cell lines of epithelial cells derived from the thick ascending limb of Henle's loop.
The use of primary cell cultures derived from defined locations of the kidney has enabled the study of certain kidney cell type-specific characteristics under defined environmental conditions. The use of primary cell cultures, however, has a number of inherent disadvantages, many of which may be overcome by the use of differentiated cell lines of defined origin. In this paper I describe in detail an approach to: (a) the isolation and culture of primary cultures derived from the thick ascending limb of Henle's loop (TALH), and (b) the production of differentiated cell lines by the transfection of these primary cell cultures with early region SV40 virus genes. The characteristics of these cultures and other TALH-derived cell lines are described.
D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D004847:Epithelial Cells; D007473:Ion Channels / Q000378:metabolism; D007684:Kidney Tubules / Q000166:cytology*; D008138:Loop of Henle / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D011817:Rabbits; D013539:Simian virus 40; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014162:Transfection
D007473:Ion Channels; D000254:Sodium-Potassium-Exchanging ATPase