Tissue culture studies of Schwann cell proliferation and differentiation.
Neonatal rat sciatic nerve Schwann cells in monolayer culture are stimulated to proliferate and to express a lipid and a protein characteristic of myelin by agents which raise intracellular cyclic adenosine 3',5'-monophosphate. Both glial growth factor and axolemmal fragments increase the rate of mitosis of cultured rat and human Schwann cells. Rat Schwann cell mitosis is enhanced by a soluble factor produced by concanavalin A-stimulated blood mononuclear cells and inhibited by lead salts. Schwann-like cells cultured from human dermal and plexiform neurofibromas resemble normal human Schwann cells in phenotype and response to mitogens.
D000818:Animals; D000831:Animals, Newborn; D001369:Axons / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002469:Cell Separation; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D003470:Culture Media; D046508:Culture Techniques; D000242:Cyclic AMP / Q000031:analogs & derivatives; D005353:Fibronectins / Q000494:pharmacology; D020904:Glia Maturation Factor; D006801:Humans; D007797:Laminin / Q000494:pharmacology; D007854:Lead / Q000494:pharmacology; D008222:Lymphokines / Q000494:pharmacology; D004676:Myelin Basic Protein / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000494:pharmacology; D009456:Neurofibromatosis 1 / Q000473:pathology; D051381:Rats; D012583:Schwann Cells / Q000166:cytology* / Q000187:drug effects; D012584:Sciatic Nerve / Q000166:cytology
D003470:Culture Media; D005353:Fibronectins; D020904:Glia Maturation Factor; D007797:Laminin; D008222:Lymphokines; D004676:Myelin Basic Protein; D009419:Nerve Tissue Proteins; D005576:Colforsin; D007854:Lead; D000242:Cyclic AMP