Heterogeneity of calcium channels from a purified dihydropyridine receptor preparation.
Dihydropyridine receptors were purified from rabbit skeletal muscle transverse tubule membranes and incorporated into planar lipid bilayers. Calcium channels from both the purified dihydropyridine receptor preparation and the intact transverse tubule membranes exhibited two sizes of unitary currents, corresponding to conductances of 7 +/- 1 pS and 16 +/- 3 pS in 80 mM BaCl2. Both conductance levels were selective for divalent cations over monovalent cations and anions. Cadmium, an inorganic calcium channel blocker, reduced the single channel conductance of calcium channels from the purified preparation. The organic calcium channel antagonist nifedipine reduced the probability of a single channel being open with little effect on the single channel conductance. The presence of two conductance levels in both the intact transverse tubule membranes and the purified dihydropyridine receptor preparation suggests that the calcium channel may have multiple conductance levels or that multiple types of calcium channels with closely related structures are present in transverse tubule membranes.
D000818:Animals; D002121:Calcium Channel Blockers / Q000378:metabolism; D015220:Calcium Channels; D004553:Electric Conductivity; D007473:Ion Channels / Q000502:physiology* / Q000648:ultrastructure; D017275:Isradipine; D007700:Kinetics; D008051:Lipid Bilayers; D009132:Muscles / Q000502:physiology*; D010069:Oxadiazoles / Q000378:metabolism; D011817:Rabbits; D011978:Receptors, Nicotinic / Q000302:isolation & purification / Q000502:physiology*
D002121:Calcium Channel Blockers; D015220:Calcium Channels; D007473:Ion Channels; D008051:Lipid Bilayers; D010069:Oxadiazoles; D011978:Receptors, Nicotinic; D017275:Isradipine