title: Modulation of carcinoembryonic antigen release by HT-29 colon carcinoma line in the presence of different agents.
abstract: In this study we followed the effects of various differentiating agents on the expression of carcinoembryonic antigen (CEA) released into the medium by a colon carcinoma cell line HT-29. Butyric acid 1 mM markedly increased the level of CEA (12-fold in comparison to control levels). 12-O-tetradecanoyl-phorbol-13-acetate (TPA) 50 ng/ml and 5-azacytidine 4 x 10(-6) M increased the amount of CEA, 2- and 1.5-fold respectively. On the other hand retinoic acid 10(-5) M, N methyl-formamide 1% and N,N hexamethylene bisacetamide 2.5 mM decreased CEA 2-, 4- and 3-fold respectively. Our results emphasize that various differentiating agents affect CEA levels differently. Thus changes in CEA levels appear not to be reliable as a marker of a more differentiated phenotype.
mesh_terms: D000081:Acetamides / Q000494:pharmacology; D001374:Azacitidine / Q000494:pharmacology; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002272:Carcinoembryonic Antigen / Q000032:analysis*; D002454:Cell Differentiation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000276:immunology*; D004357:Drug Synergism; D005559:Formamides / Q000494:pharmacology; D006801:Humans; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D000081:Acetamides; D002087:Butyrates; D002272:Carcinoembryonic Antigen; D005559:Formamides; D020148:Butyric Acid; D014212:Tretinoin; C014026:hexamethylene bisacetamide; D001374:Azacitidine; D013755:Tetradecanoylphorbol Acetate; C002950:methylformamide