Effect of cutting or crushing the rat sciatic nerve on synthesis of substance P by isolated L5 dorsal root ganglia.
Following cutting or crushing the rat sciatic nerve, synthesis of substance P by L5 dorsal root ganglia in vitro was reduced respectively to 20 and 40 per cent of control values. By day 64 the ability to synthesise substance P had been fully restored in crushed neurones but in cut neurones remained at a low level. We conclude that substance P synthesis is a more sensitive index of the effect of nerve injury than is substance P content and further that regenerating axons are able to support substance P synthesis before they reach their target tissue.
D000818:Animals; D000841:Anisomycin / Q000494:pharmacology; D005727:Ganglia, Spinal / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D009409:Nerve Crush; D051381:Rats; D011919:Rats, Inbred Strains; D012584:Sciatic Nerve / Q000378:metabolism*; D013373:Substance P / Q000096:biosynthesis* / Q000378:metabolism; D013997:Time Factors
D013373:Substance P; D000841:Anisomycin