title: Polyamine metabolism in an obligately alkalophilic Bacillus alcalophilus that grows at pH 11.0.
abstract: Bacillus alcalophilus, an obligately alkalophilic bacterium that grows at pH 11.0, has an intracellular pH of 9.5 or less. Unlike all other living organisms, polyamines (putrescine, spermidine and spermine) in B. alcalophilus, if present, will be largely unprotonated. HPLC analysis indicated that spermidine is the major polyamine in B. alcalophilus, accounting for more than 90% of total polyamines, and the level of spermidine varies during growth. Ornithine decarboxylase activity was not detectable in B. alcalophilus under all conditions examined. When [3H]arginine was added to the culture medium, the radioactivity can be recovered from polyamine pool; the distribution is 3% for putrescine, 94% for spermidine, and 3% for spermine, suggesting the presence of arginine pathway for polyamine biosynthesis. The polyamine transport system in B. alcalphilus appears to be Na+-dependent and is highly sensitive to the inhibition of gramicidin S and valinomycin.
mesh_terms: D001120:Arginine / Q000378:metabolism; D001407:Bacillus / Q000254:growth & development / Q000378:metabolism*; D001693:Biological Transport, Active / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000378:metabolism; D006096:Gramicidin / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009952:Ornithine / Q000378:metabolism; D009955:Ornithine Decarboxylase / Q000378:metabolism; D011073:Polyamines / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism; D012964:Sodium / Q000494:pharmacology; D013095:Spermidine / Q000378:metabolism; D013096:Spermine / Q000378:metabolism; D014634:Valinomycin / Q000494:pharmacology
chemical_list: D011073:Polyamines; D006096:Gramicidin; D014634:Valinomycin; D013096:Spermine; D001120:Arginine; D012964:Sodium; D009952:Ornithine; D009955:Ornithine Decarboxylase; D013095:Spermidine; D011700:Putrescine