Effect of a high NaCl diet on the active mechanisms of Na+ extrusion in rat kidney.
Healthy male rats were exposed to a chronic ingesta of an isotonic NaCl solution during a period of four months. Two different preparations were utilized to study the effect of this treatment on the active mechanisms of Na+ reabsorption of kidney proximal tubular cells: outermost kidney cortex slices (rich in proximal tubules) and inside-out basolateral plasma membrane vesicles prepared from the same tissue. It was found that the activity of the ouabain-insensitive, Na-pump of basolateral plasma membranes of kidney proximal tubular cells was increased in about 70%, whereas the ouabain-sensitive, Na,K-pump activity did not change with the experimental treatment. These results represent a strong support to the two Na-pump's hypothesis since the treatment affected the Na-pump differentially without affecting the Na,K-pump.
D000042:Absorption; D000818:Animals; D004032:Diet; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D010101:Oxygen Consumption / Q000187:drug effects; D011188:Potassium / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000378:metabolism*; D012965:Sodium Chloride / Q000008:administration & dosage*; D013997:Time Factors
D007473:Ion Channels; D012965:Sodium Chloride; D012964:Sodium; D011188:Potassium