title: Detection of heat-stable enterotoxigenic Escherichia coli by hybridization with an RNA transcript probe.
abstract: Heat-stable enterotoxigenic Escherichia coli was identified by nucleotide hybridization with RNA transcripts of the gene encoding heat-stable A-2 enterotoxin. Radiolabeled enterotoxin gene RNA transcripts are easier to prepare and avoid the preparation of cloned DNA probes that can be nonspecific if they contain cloning vector DNA.
mesh_terms: D001427:Bacterial Toxins / Q000096:biosynthesis / Q000235:genetics*; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis*; D004768:Enterotoxins / Q000096:biosynthesis / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012329:RNA, Bacterial / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D012329:RNA, Bacterial; C034151:heat stable toxin (E coli); D004262:DNA Restriction Enzymes