title: Amino acid sequence and gene organization of cytokeratin no. 19, an exceptional tail-less intermediate filament protein.
abstract: We have isolated a cDNA clone from a bovine bladder urothelium library which encodes the smallest intermediate filament (IF) protein known, i.e. the simple epithelial cytokeratin (equivalent to human cytokeratin 19) previously thought to have mol. wt 40,000. This clone was then used to isolate the corresponding gene from which we have determined the complete nucleotide sequence and deduced the amino acid sequence of the encoded protein. This cytokeratin of 399 amino acids (mol. wt 43,893) is identified as a typical acidic (type I) cytokeratin but differs from all other IF proteins in that it does not show the carboxyterminal, non-alpha-helical tail domain. Instead it contains a 13 amino acids extension of the alpha-helical rod. The gene encoding cytokeratin 19 is also exceptional. It contains only five introns which occur in positions corresponding to intron positions in other IF protein genes. However, an intron which in all other IF proteins demarcates the region corresponding to the transition from the alpha-helical rod into the non-alpha-helical tail is missing in the cytokeratin 19 gene. Using in vitro reconstitution of purified cytokeratin 19 we show that it reacts like other type I cytokeratins in that it does not form, in the absence of a type II cytokeratin partner, typical IF. Instead it forms 40-90 nm rods of 10-11 nm diameter which appear to represent lateral associations of a number of cytokeratin molecules. Our results demonstrate that the non-alpha-helical tail domain is not an indispensable feature of IF proteins. The gene structure of this protein provides a remarkable case of a correlation of a change in protein conformation with an exon boundary.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002918:Chymotrypsin; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D004720:Endonucleases; D004848:Epithelium / Q000378:metabolism; D005796:Genes; D007381:Intermediate Filament Proteins / Q000235:genetics; D007633:Keratins / Q000235:genetics*; D010446:Peptide Fragments / Q000032:analysis; D015719:Single-Strand Specific DNA and RNA Endonucleases; D001743:Urinary Bladder / Q000378:metabolism
chemical_list: D007381:Intermediate Filament Proteins; D010446:Peptide Fragments; D007633:Keratins; D004247:DNA; D004720:Endonucleases; D015719:Single-Strand Specific DNA and RNA Endonucleases; D002918:Chymotrypsin