The influence of furosemide and Co2+ on electrolyte and water transport in newt distal tubule and frog skin.
The technique of stationary microperfusion of the early distal tubule used in experiments on male newts (Triturus vulgaris) revealed the intratubular effect of CoCl2 and furosemide on the ion transport. CoCl2 in concentration 5 X 10(-4) M increased Na and Cl reabsorption, reduced Ca, Mg and K reabsorption, and increased (TFexp/TFo)in from 1.14 +/- 0.04 to 1.68 +/- 0.27 (p less than 0.001). CoCl2 in concentration 5 X 10(-5) M enhanced Na and Cl reabsorption, without affecting transport of other ions. 6 X 10(-4) M furosemide decreased reabsorption of water, Na, Cl, Ca and Mg. The combined effect of Co2+ and furosemide inhibited reabsorption of Mg and Ca to a greater extent, but stimulated Na reabsorption. Experiments on frog skin showed that being added to the apical membrane of cells, Co2+ stimulates short circuit current and potential differences, whereas furosemide reducing Cl absorption induces only hyperpolarization. Experimental findings allow to assume that Co2+ on the outer surface of the apical membrane of the distal tubule cells blocks the selective Ca and Mg channels and activates Na channels.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D003035:Cobalt / Q000494:pharmacology*; D004573:Electrolytes / Q000378:metabolism*; D005665:Furosemide / Q000494:pharmacology*; D007473:Ion Channels / Q000187:drug effects; D007684:Kidney Tubules / Q000378:metabolism*; D007686:Kidney Tubules, Distal / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D011896:Rana temporaria; D012867:Skin / Q000187:drug effects / Q000378:metabolism*; D014319:Triturus; D014867:Water / Q000378:metabolism*
D004573:Electrolytes; D007473:Ion Channels; D014867:Water; D003035:Cobalt; D005665:Furosemide