Isoprinosine restores in vitro T lymphocyte functions of cyclophosphamide immunosuppressed mice.
The effect and the mechanism of action of isoprinosine has been investigated in several models of in vitro activation of lymphocytes. Isoprinosine added to spleen cell cultures enhanced lymphocyte proliferation induced by concanavalin A or allogeneic stimulation as well as the generation of allospecific cytotoxic T cells. The effect of isoprinosine on T lymphocyte proliferation in vitro was specially marked when mice were treated with cyclophosphamide (75-200 mg/kg) 16-24 h before the onset of cultures. No effect was observed on B cell proliferation to LPS. Addition of inosine or adenosine also enhanced proliferation of cells from both normal and cyclophosphamide treated mice. Isoprinosine and inosine and, more markedly, adenosine, augmented interleukin-2 activity in concanavalin A supernatants of spleen cells from the same animals.
D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D003520:Cyclophosphamide; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D007111:Immunity, Cellular / Q000187:drug effects*; D007165:Immunosuppression Therapy; D007288:Inosine / Q000031:analogs & derivatives*; D007542:Inosine Pranobex / Q000494:pharmacology*; D007376:Interleukin-2 / Q000096:biosynthesis; D007694:Killer Cells, Natural / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D011684:Purine Nucleosides / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects*
D007376:Interleukin-2; D011684:Purine Nucleosides; D003208:Concanavalin A; D007288:Inosine; D003520:Cyclophosphamide; D007542:Inosine Pranobex