T lymphocyte recognition of acetylcholine receptor: localization of the full T cell recognition profile on the extracellular part of the alpha chain of Torpedo californica acetylcholine receptor.
A series of eighteen consecutive overlapping synthetic peptides, of uniform size and overlaps, which encompass the entire extracellular part (residues 1-210) of the Torpedo californica acetylcholine receptor alpha chain were examined in vitro for their ability to stimulate lymph node cells from acetylcholine receptor-primed C57BL/6 (H-2b), C3H/He (H-2k), SWR (H-2q) and SJL (H-2s) mice. The recognition sites (T sites) by acetylcholine receptor-primed lymph node cells from these mouse strains resided within six regions on the extracellular part of the alpha chain. Three of the regions recognized by T cells coincided with regions recognized by antibodies (i.e. B cells) and one of these three regions also coincided with an alpha-neurotoxin-binding region. It is noteworthy that, in addition to sites recognized by both T and B cells, the protein has at least two sites which are recognized exclusively by T cells and to which no detectable antibody responses are directed.
D000818:Animals; D000939:Epitopes; D005110:Extracellular Space; D008213:Lymphocyte Activation; D046911:Macromolecular Substances; D051379:Mice; D008815:Mice, Inbred Strains; D009842:Oligopeptides / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D011978:Receptors, Nicotinic / Q000276:immunology*; D013329:Structure-Activity Relationship; D013601:T-Lymphocytes / Q000276:immunology*; D014101:Torpedo
D000939:Epitopes; D046911:Macromolecular Substances; D009842:Oligopeptides; D011948:Receptors, Antigen, T-Cell; D011978:Receptors, Nicotinic