[A method for demonstrating mycoplasmas].
Bisbenzimid-marked continuous cell lines were investigated via fluorescent microscopy. The presence of Mycoplasma DNA was demonstrated in the cytoplasm with some of them. The method employed for the demonstration of Mycoplasma contamination is readily applicable, and could be used for control on continuous cell lines.
D000818:Animals; D001431:Bacteriological Techniques; D006690:Bisbenzimidazole; D002478:Cells, Cultured / Q000382:microbiology; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D009174:Mycoplasma / Q000302:isolation & purification*; D014776:Virus Cultivation
D006690:Bisbenzimidazole
Metod za dokazvane na mikoplazmi.