Preparation of target antigens specifically recognized by human natural killer cells.
In an attempt to identify target cell membrane molecules recognized by natural killer (NK) cells, artificial membranes were prepared from detergent-solubilized plasma membranes of NK target cells and synthetic lipids. Such reconstituted membranes from human and rat NK target cells were shown to inhibit both human and rat NK-target cell conjugates in a species-specific fashion; these reconstituted membranes failed to inhibit NK cytotoxicity. The detergent-solubilized material from the human NK target K562 was subjected to various procedures prior to reconstitution and the conjugate inhibition assay. Conjugate inhibitory activity was lost upon trypsin digestion and incubation at 65 degrees C. This inhibition activity was absorbed to concanavalin A agarose and could subsequently be eluted with alpha-methylmannoside, resulting in approximately 20-fold purification. Gel filtration of this material on an AcA-34 column in detergent gave a broad activity peak with maximal activity in the molecular weight range of 30,000-165,000. Gel electrophoresis of purified membranes demonstrated multiple molecular weight bands in lipid membranes. The K562 membrane material, purified by concanavalin A agarose and gel filtration, inhibited conjugates between human NK cells and any of four human target cells, but not of conjugates with (1) human large granular lymphocytes and antibody-coated mouse tumor cells nor (2) rat NK cells and their target cells. Thus the purified glycoproteins from K562 retain the property of specific inhibition of human NK-target conjugates.
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology / Q000302:isolation & purification*; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D002850:Chromatography, Gel; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000302:isolation & purification; D006801:Humans; D066298:In Vitro Techniques; D007694:Killer Cells, Natural / Q000276:immunology*; D037102:Lectins; D008567:Membranes, Artificial; D051379:Mice; D008970:Molecular Weight; D051381:Rats
D000954:Antigens, Surface; D000939:Epitopes; D037102:Lectins; D008567:Membranes, Artificial