Immunohistochemical studies on colloid bodies (Civatte bodies) in oral lesions of discoid lupus erythematosus.
By electron microscopy colloid bodies have been shown to be derived from epithelial cells. It has been suggested, however, that connective tissue cells or components from the basement membrane zone contributed to the formation of colloid bodies. In order to examine these possibilities we stained oral lesions of discoid lupus erythematosus (DLE) with antibodies against intermediate filaments (keratin, vimentin), basement membrane components (laminin, collagen type IV) and fibronectin. IgM was used as a marker for colloid bodies. Colloid bodies were stained positive for keratin, whereas vimentin was never found in colloid bodies. Laminin and collagen type IV were occasionally seen in their periphery probably owing to adherence of basement membrane fragments during apoptosis. Fibronectin was frequently seen at the entire periphery of colloid bodies which may facilitate their elimination by macrophages. In conclusion, connective tissue cells or basement membrane components do not seem to contribute to the formation of colloid bodies in oral DLE.
D000911:Antibodies, Monoclonal; D001485:Basement Membrane / Q000378:metabolism; D003094:Collagen / Q000032:analysis; D003102:Colloids / Q000378:metabolism*; D003594:Cytoplasmic Granules / Q000378:metabolism; D006801:Humans; D007382:Intermediate Filaments / Q000378:metabolism; D007633:Keratins / Q000032:analysis; D007797:Laminin / Q000032:analysis; D008179:Lupus Erythematosus, Discoid / Q000378:metabolism*; D009059:Mouth Diseases / Q000378:metabolism*; D009061:Mouth Mucosa / Q000378:metabolism; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D003102:Colloids; D007797:Laminin; D007633:Keratins; D003094:Collagen