title: High and testosterone-dependent glucose 6-phosphatase activity in epithelium of mouse seminal vesicle.
abstract: For study of the mechanism of seminal fructogenesis, glucose 6-phosphatase activity was examined cytochemically (a method modified from that of Wachstein and Meisel) and biochemically (the method of Leskes et al.) in seminal vesicles from normal, castrated, and castrated and testosterone-treated mice. The reaction product for the activity was localized in the endoplasmic reticulum and nuclear envelope of all cell types composing the seminal vesicle. In normal seminal vesicle, the reaction product was apparently more abundant in columnar and basal cells than in other cell types. Ten, 20, and 30 days after castration, the abundant amount of reaction product in columnar and basal cells decreased to the level in other cell types. In animals treated with testosterone after castration, however, the reaction product in columnar and basal cells remained abundant. If fructose 6-phosphate was added to the reaction medium in place of glucose 6-phosphate, the amount and pattern of deposition of the reaction product did not change. Changes in biochemical activity in castrated or castrated and testosterone-treated animals paralleled the cytochemical results. The results show that the high activity in columnar and basal cells is under the control of testosterone, and the role of this enzyme is probably to release fructose into the seminal fluid.
mesh_terms: D000818:Animals; D004721:Endoplasmic Reticulum / Q000201:enzymology; D004848:Epithelium / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D005952:Glucose-6-Phosphatase / Q000378:metabolism*; D007978:Levamisole / Q000494:pharmacology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009685:Nuclear Envelope / Q000201:enzymology; D009919:Orchiectomy; D011190:Potassium Cyanide / Q000494:pharmacology; D011506:Proteins / Q000032:analysis; D012669:Seminal Vesicles / Q000032:analysis / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D013194:Staining and Labeling; D013739:Testosterone / Q000502:physiology*
chemical_list: D011506:Proteins; D007978:Levamisole; D013739:Testosterone; D005952:Glucose-6-Phosphatase; D011190:Potassium Cyanide