Contamination of a monoclonal antibody with LDH-virus causes interferon induction.
Interferon induction occurred unexpectedly during an in vivo study using a mouse monoclonal antibody. The interferon was typed alpha/beta and the titer reached a maximum at 24 hours in contrast with other inducers. Similar results were obtained with a virus pool derived from the antibody and with a LDV reference strain. MAP-testing of the monoclonal antibody revealed contamination with lactate dehydrogenase virus (LDV). The production of IFN seems to be controlled genetically. This experimental error demonstrates the importance of an appropriate quality control of biological materials.
D000818:Animals; D000911:Antibodies, Monoclonal; D004340:Drug Contamination; D005260:Female; D007372:Interferons / Q000096:biosynthesis*; D007772:Lactate dehydrogenase-elevating virus / Q000302:isolation & purification / Q000502:physiology; D051379:Mice; D008815:Mice, Inbred Strains / Q000378:metabolism*; D011786:Quality Control
D000911:Antibodies, Monoclonal; D007372:Interferons