[Effect of the level of free-radical lipid peroxidation on the ribonucleotide reductase activity of liver tissue].
The dynamics of RNA and DNA synthesis as well as the activity of free radical processes in rat liver during the first 40 hours after partial hepatectomy was studied. It was shown that RNA synthesis activation follows the activation of lipid peroxidation (LPO), whereas the DNA synthesis activation follows the decrease of the LPO level. These facts are suggestive of the dependence of the ribonucleotide reductase (RNR) activity on free radical processes. This observation was confirmed by a RNR activity analysis of regenerating liver homogenates. The activity peak was shown to precede the peak of DNA synthesis. Evidence for free radical RNR suppression was also obtained in direct experiments, using intact animal liver homogenates, to which a natural antioxidant (tocopherol) was added.
D000818:Animals; D004247:DNA / Q000096:biosynthesis; D005609:Free Radicals; D008054:Lipid Peroxides / Q000378:metabolism*; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D008115:Liver Regeneration; D008297:Male; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D012264:Ribonucleotide Reductases / Q000378:metabolism*; D014810:Vitamin E / Q000494:pharmacology
D005609:Free Radicals; D008054:Lipid Peroxides; D014810:Vitamin E; D012313:RNA; D004247:DNA; D012264:Ribonucleotide Reductases
Vliianie urovnia svobodnoradikal'nykh protsessov okisleniia lipidov na ribonukleotidreduktaznuiu aktivnost' tkani pecheni.