title: Production and preliminary characterization of murine monoclonal antibodies to Ichthyophthirius multifiliis, a protozoan parasite of fish.
abstract: An initial panel of 34 hybridomas, each secreting antibodies reactive with an infective theront stage of an Ichthyophthirius multifiliis isolate, was produced. Three of these cell lines, each producing immunoglobulin M class antibodies, were cloned by limiting dilution and were expanded as ascites-producing tumors in syngeneic mice. Three monoclonal antibodies (MAB) reacted with intact whole theronts in an enzyme-linked immunosorbent assay to dilutions of 1:10,000 in ascitic fluids and had a similar pattern of surface and cytoplasmic staining in indirect immunofluorescent tests. Only antigen specified by MAB E6 could be characterized by acrylamide gel electrophoresis and immunoblotting; initial data indicated a molecular weight of 200,000. Physicochemical properties of the determinants recognized by the 3 MAB were tested by pronase digestion and periodate oxidation. Seemingly, a protein, glycoprotein, and carbohydrate were recognized by MAB E6, FE10, and AC8, respectively.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology*; D000953:Antigens, Protozoan / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D056890:Eukaryota / Q000276:immunology*; D005260:Female; D005393:Fish Diseases / Q000276:immunology / Q000517:prevention & control; D005399:Fishes; D005455:Fluorescent Antibody Technique; D006825:Hybridomas; D007075:Immunoglobulin M / Q000032:analysis / Q000096:biosynthesis / Q000276:immunology*; D007158:Immunologic Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D011528:Protozoan Infections / Q000276:immunology / Q000517:prevention & control; D011529:Protozoan Infections, Animal
chemical_list: D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D000939:Epitopes; D007075:Immunoglobulin M