[Production of monoclonal antibodies to tissue-specific antigens of human skin epithelium].
Immunization of BALB/c mice with non-type-specific protein antigens of the cellular wall of group A streptococcus and formalin-treated streptococcal culture resulted in stimulating production of polyclonal autoantibodies to antigens of the epithelium of human and murine skin. As a result of hybridoma technique using splenic cells of immunized animals, monoclonal antibodies to different antigens of epidermal cell cytoplasm, i.e. antigen of basal cells, antigen of differentiated cell layers (spinous and granular) and antigen common to cells of all epidermal layers, were obtained. The immunofluorescence tests on monoclonal antibodies to epidermal basal cell antigen showed them to engage cells only of tumors histogenetically associated with the epidermal tegumental epithelium.
D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000941:Antigens / Q000276:immunology*; D004848:Epithelium / Q000276:immunology; D000939:Epitopes; D006801:Humans; D012867:Skin / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000941:Antigens; D000939:Epitopes
Poluchenie monoklonal'nykh antitel k tkanespetsificheskim antigenam épiteliia kozhi cheloveka.