[Preparation and characterization of antisera specific to various polypeptide domains corresponding to the v-ets oncogene of the avian leukemia virus E26].
We prepared antisera to three distinct portions of the v-ets oncogene of the avian leukemia virus E26. An antiserum directed against the middle v-ets-encoded domain identifies in different chicken cell lines and normal tissues a c-ets-encoded protein of Mr 54,000 (P54c-ets) and three proteins of Mr 60,000 62,000 and 64,000 partially related to P54c-ets. Antisera directed against the aminoterminal v-ets-encoded domain failed to precipitate P54c-ets or P60/P64. Thus, the E26 specific v-ets oncogene displays a complex structure that includes several distinct portions, the genetic origin of which could be different.
D000818:Animals; D000936:Antigen-Antibody Complex; D001354:Avian Leukosis Virus / Q000235:genetics*; D002460:Cell Line; D002645:Chickens; D000939:Epitopes / Q000032:analysis*; D005814:Genes, Viral; D007106:Immune Sera; D009857:Oncogenes; D014764:Viral Proteins / Q000276:immunology*
D000936:Antigen-Antibody Complex; D000939:Epitopes; D007106:Immune Sera; D014764:Viral Proteins
Préparation et caractérisation d'antisera specifiques de différents domaines polypeptidiques correspondant à l'oncogène v-ets du virus aviaire de leucémies aiguës E26.