title: Regulation of the expression of proto-oncogene c-src by alternative RNA splicing in chicken skeletal muscle.
abstract: Expression of the c-src locus in skeletal muscle of the chicken was found to be different from other tissues. A 4 kilobases (kb) cellular src (c-src) mRNA, which most likely codes for pp60c-src, is expressed in various chicken tissues examined, and most of its approximately 2.2 kb non-coding sequences are present at the 3' end of the RNA molecule. Whereas the 4 kb RNA is also expressed in embryonic chicken muscle, it disappears shortly before hatching and is replaced by a smaller-sized class of c-src mRNAs of 2.8 to 3.3 kb. The transition of expression from the 4 kb to the 2.8-3.3 kb RNA in skeletal muscle occurs shortly before hatching of the chicken and persists into adulthood. The two classes of the c-src mRNA share sequences both within the 5' coding and the 3' non-coding regions of pp60c-src, however, the 2.8 to 3.3 kb RNA lacks most of the kinase domain, but it contains upstream c-src sequences missing in the 4 kb RNA. The two classes of c-src mRNA most likely are generated by alternative splicing, and the smaller c-src RNA most likely codes for a product other than tyrosine protein kinase.
mesh_terms: D000367:Age Factors; D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics*; D003001:Cloning, Molecular; D008970:Molecular Weight; D009132:Muscles / Q000196:embryology / Q000502:physiology*; D009693:Nucleic Acid Hybridization; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016392:Proto-Oncogene Proteins pp60(c-src); D011519:Proto-Oncogenes; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D014018:Tissue Distribution
chemical_list: D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D016392:Proto-Oncogene Proteins pp60(c-src)