Chemical identification of a tumor-derived angiogenic factor.
Neoplasms produce substances that induce blood vessel formation (angiogenesis). Fractions from ethanol extracts of the Walker 256 carcinoma were isolated by silica column chromatography and C18 reversed-phase high-performance liquid chromatography. Two of the isolated fractions induced neovascularization when tested in the rabbit corneal micropocket assay. One of the fractions was identified as nicotinamide by desorption-electron impact mass spectrometry, nuclear magnetic resonance spectroscopy, and gas chromatography-mass spectrometry. The second active fraction contained nicotinamide as part of a more complex, as yet unidentified, molecular arrangement. Microgram quantities of commercial nicotinamide induced neovascularization in the corneal micropocket assay and in the chick chorioallantoic membrane assay.
D043925:Angiogenesis Inducing Agents / Q000302:isolation & purification* / Q000494:pharmacology; D000818:Animals; D002279:Carcinoma 256, Walker / Q000503:physiopathology*; D002478:Cells, Cultured; D002642:Chick Embryo; D003315:Cornea / Q000098:blood supply; D004727:Endothelium / Q000166:cytology / Q000187:drug effects; D008401:Gas Chromatography-Mass Spectrometry; D006133:Growth Substances / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D051379:Mice; D009389:Neovascularization, Pathologic; D009536:Niacinamide / Q000302:isolation & purification / Q000494:pharmacology
D043925:Angiogenesis Inducing Agents; D006133:Growth Substances; D009536:Niacinamide