Effects of 3'-methyl-4-dimethylaminoazobenzene on RNA processing in rat liver nuclei.
Treatment of rats with the hepatocarcinogen 3'-methyl-4-dimethylaminoazobenzene (3'MeDAB) leads to the accumulation of polyadenylated RNA in rat liver nuclei. This polyadenylated nuclear RNA contains more double-stranded stretches than polyadenylated nuclear RNA isolated from liver of control animals. Treatment with 3'MeDAB also results in an increase of sequences homologous to small chromatin-associated RNA (fr 3-RNA) in the polyadenylated nuclear RNA and in the appearance of these sequences in polyadenylated nuclear RNA which contains some double-stranded stretches. The last mentioned fraction of nuclear RNA from liver of control animals did not hybridize with a DNA probe for fr 3-RNA. It is proposed that, perhaps as a result of inhibition of RNA processing by 3'MeDAB, the transient stages of RNA processing can be observed in animals treated with this compound.
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D008749:Methyldimethylaminoazobenzene / Q000494:pharmacology*; D012313:RNA / Q000378:metabolism*; D012342:RNA, Small Nuclear / Q000378:metabolism; D051381:Rats; D004124:p-Dimethylaminoazobenzene / Q000031:analogs & derivatives*
D012342:RNA, Small Nuclear; D008749:Methyldimethylaminoazobenzene; D012313:RNA; D004124:p-Dimethylaminoazobenzene