[Isolation of different variants of Pseudomonas aeruginosa anatoxin and their characteristics].
The conditions for the detoxification of the crude preparations of P. aeruginosa exotoxin A, obtained by the cultivation of strain PA-7 in Martin's broth, have been studied, and the schemes for obtaining nontoxic, stable, specifically antigenic preparations of toxoid from exotoxins A with different degrees of purification have been developed. Toxoid obtained by formalin treatment on the level of a crude preparation with its subsequent purification and additional detoxification with formalin in the presence of lysin has been shown to possess high immunogenic potency. The preparation has been found to induce immune response and to ensure the protection of experimental animals challenged not only with the lethal dose of exotoxin A, but also with P. aeruginosa toxigenic and protease-producing strains.
D036002:ADP Ribose Transferases; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D000942:Antigens, Bacterial / Q000032:analysis; D001427:Bacterial Toxins; D001431:Bacteriological Techniques; D004355:Drug Stability; D000939:Epitopes / Q000032:analysis; D005098:Exotoxins / Q000302:isolation & purification; D007106:Immune Sera / Q000302:isolation & purification; D007114:Immunization; D007928:Lethal Dose 50; D051379:Mice; D011550:Pseudomonas aeruginosa / Q000276:immunology; D011817:Rabbits; D013997:Time Factors; D014121:Toxoids / Q000276:immunology / Q000302:isolation & purification* / Q000633:toxicity; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D000939:Epitopes; D005098:Exotoxins; D007106:Immune Sera; D014121:Toxoids; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
Poluchenie razlichnykh variantov anatoksina Pseudomonas aeruginosa i ikh kharakteristiki.