[Effect of non-histone chromatin proteins on the transcription of globin genes].
Globin gene transcription was studied in vitro using chromatin of normal and Rauscher virus-transformed erythroid cells. For this purpose the reconstituted chromatin was obtained and used as a template for RNA synthesis. The RNA-transcript was then analysed for the presence of globin mRNA sequences. When chromatin was reconstituted using DNA from Rauscher-transformed erythroblasts and reticulocyte nonhistone proteins they enhanced transcription. Most of the globin gene activation was due to the nonhistone protein fraction eluted from hydroxylapatite in 0.05 M Na-phosphate and the RNA-transcript in this case contained the largest quantity of globin sequences.
D000818:Animals; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D004247:DNA / Q000235:genetics; D004900:Erythroblasts / Q000378:metabolism* / Q000382:microbiology; D005914:Globins / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000235:genetics; D011925:Rauscher Virus; D012156:Reticulocytes / Q000378:metabolism* / Q000382:microbiology; D014158:Transcription, Genetic
D002868:Chromosomal Proteins, Non-Histone; D012313:RNA; D005914:Globins; D004247:DNA
Vliianie negistonovykh belkov khromatina na transkriptsiiu globinovykh genov.