Image analysis combined with quantitative cytochemistry. Results and instrumental developments for cancer diagnosis.
This paper describes the application of image analysis combined with a quantitative staining method for the analysis of cervical specimens. The image analysis is carried out with the Leyden Television Analysis System, LEYTAS, of which two versions are described. LEYTAS-1 as well as LEYTAS-2 have both been designed with a high degree of flexibility and interaction facilities. A much wider range of image analysis programs is however, possible with LEYTAS-2, enabling many applications. LEYTAS-1, the earlier version, consists of a Leitz microscope with automated functions, a TV camera, the Texture Analysis System (TAS, Leitz), a four-bit grey value memory and a minicomputer (PDP 11/23). Using this instrumentation 1,500 cervical smears prepared from cell suspensions and stained with acriflavin-Feulgen-Sits have been analysed in a completely automated procedure. Image transformations working in parallel on entire fields, have been used for cell selection and artefact rejection. Resulting alarms, consisting of selected single cells and non-rejected artefacts are stored in the grey value memory, which is displayed on a TV monitor. This option allows visual interaction after the machine diagnosis has been made. The machine diagnosis was correct in 320 out 321 specimens with a severe dysplasia or more serious lesion. The false positive rate in 561 morphologically negative specimens (normal and inflammation) was 16% (machine diagnosis). Visual interaction by subtracting the visually recognized false alarms from the total number of alarms reduces the false positive rate to 11%.(ABSTRACT TRUNCATED AT 250 WORDS)
D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D000167:Acriflavine; D004396:Coloring Agents; D003201:Computers; D005260:Female; D006801:Humans; D007249:Inflammation / Q000473:pathology; D011230:Precancerous Conditions / Q000175:diagnosis* / Q000473:pathology; D012394:Rosaniline Dyes; D013194:Staining and Labeling; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000473:pathology
D004396:Coloring Agents; C007722:Feulgen stain; D012394:Rosaniline Dyes; D000167:Acriflavine; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid