DNAase-I-hypersensitive sites in the mouse albumin gene.
We have analyzed the DNAase I sensitivity of the mouse alpha-fetoprotein and albumin structural genes from fetal liver, adult liver and kidney. The albumin gene shows distinct hypersensitive sites in adult liver in addition to an overall DNAase I sensitivity, but is only slightly nuclease-sensitive in fetal liver. The alpha-fetoprotein gene does not show hypersensitive sites but displays an overall DNAase I sensitivity in fetal liver; however, it is nuclease-insensitive in adult liver. Both genes are insensitive to DNAase I in kidney. The presence of DNAase-I-hypersensitive sites in the albumin structural gene correlates with extensive demethylation of the gene in adult liver.
D000418:Albumins / Q000235:genetics*; D000818:Animals; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism*; D005796:Genes; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis / Q000196:embryology; D008745:Methylation; D051379:Mice; D008807:Mice, Inbred BALB C; D009693:Nucleic Acid Hybridization; D000509:alpha-Fetoproteins / Q000235:genetics*
D000418:Albumins; D000509:alpha-Fetoproteins; D004247:DNA; D003850:Deoxyribonuclease I