Chemical and clinical studies of endogenous digitalis-like factor in hypertension.
Endogenous digitalis-like factor (endalin) was investigated by measuring the ability of rat and human plasma and urine to inhibit [3H]ouabain-specific binding, digoxin-antidigoxin antibodies interaction, and renal Na+, K+-ATPase activity. Endalin was detected in plasma (and urine) of one third of 112 patients with sustained and moderate hypertension (Na+ intake = 110 mmol/l). Endalin tended to be increased in the more pronounced hypertensives. No correlation with any other clinical and biological parameter could be detected. An activity to inhibit Na+, K+-ATPase was also detected in the rat after acute and chronic Na+ loading, in reduced renal mass-type hypertension and in SHRs as compared to WKY rats. Comparison of the plasma and urine inhibitory effects in the different tests revealed some chemical heterogeneity. However, a compound possessing the biochemical and pharmacological characteristics of digitaline was extracted from human urine. Chromatographic and spectral analysis of about 1,000 liters revealed a compound with apparent chemical homogeneity, molecular weight around 500, devoid of peptidic bound and of aliphatic structure.
D000328:Adult; D000368:Aged; D000818:Animals; D002298:Cardenolides; D004077:Digoxin; D005260:Female; D006801:Humans; D006973:Hypertension / Q000378:metabolism*; D006977:Hypertension, Renal / Q000378:metabolism; D007473:Ion Channels / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D051381:Rats; D011919:Rats, Inbred Strains; D012503:Saponins; D012964:Sodium / Q000008:administration & dosage / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors* / Q000378:metabolism
D002298:Cardenolides; D007473:Ion Channels; D012503:Saponins; C034504:digoxin-like factors; D004077:Digoxin; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase