RNA colony hybridization method.
A method for rapid screening of specific RNA sequences in recombinant colonies by hybridization in situ is presented. The method includes two consecutive steps of lytic treatment of the nitrocellulose-filter-supported colonies (10% sodium dodecyl sulfate and 3 X SSC at 65 degrees C) and hybridization with 32P-labelled specific oligodeoxynucleotides.
D001483:Base Sequence; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides; D012329:RNA, Bacterial / Q000235:genetics*; D011995:Recombination, Genetic; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D009838:Oligodeoxyribonucleotides; D012329:RNA, Bacterial