Effects of kallikrein (K), bradykinin (B) and insulin (I), on substrate metabolism in the isolated perfused rat heart.
In the isolated rat heart preparation, perfused at physiological glucose levels, effects of kallikrein (K), bradykinin (B) and insulin (I) on carbohydrate metabolism have been compared. A flow- and I-independent K-effect, obviously working via proteolytic kinin formation, was shown. While I elevated glucose-uptake into the heart preferably via accelerated transport across the membrane and increased rates of glycogen synthesis, B and K significantly activated PFK and reduced myocardial glucose uptake. The latter effect is probably due to an accelerated glycolytic flux leading to decreased G6P-levels and thereby stimulating glycogenolysis. Since aprotinin was able to abolish the K-effect, the latter may possibly be mediated via kinin release.
D000818:Animals; D007611:Aprotinin / Q000494:pharmacology; D001693:Biological Transport, Active / Q000187:drug effects; D001920:Bradykinin / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects; D006321:Heart / Q000187:drug effects; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D007610:Kallikreins / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D007328:Insulin; D006003:Glycogen; D007611:Aprotinin; D007610:Kallikreins; D005947:Glucose; D001920:Bradykinin