Monoclonal antibodies detecting different epitopes on the Forssman glycolipid hapten.
Two hybridomas, derived by fusing mouse myeloma cells with spleen cells from a rat immunized with mouse mammary tumors, have been shown to produce antibodies that recognize cell surface antigens on mesenchymal cells in a variety of tissues. Evidence presented in this report suggests that these antibodies detect overlapping epitopes on the Forssman glycolipid hapten (GalNAc alpha 1-3GalNAc beta 1-3Gal alpha 1-4Gal beta 1-4Glc beta 1-1Cer). One antibody (33B12) reacts with the terminal sugar sequence GalNAc alpha 1-3GalNAc and is specific for Forssman. The other antibody (117C9) recognizes the internal sugar sequence GalNAc beta 1-3Gal. The terminal sugar sequence GalNAc beta 1-3Gal in globoside, as well as the internal sugar sequence GalNAc beta 1-4Gal in asialo-GM1, is not recognized as an antigenic determinant by 117C9. Nevertheless, the 117C9 antibody does not react exclusively with the Forssman antigen. In a lipid extract fractionated by Folch partition of mouse mammary tumors, the antibody also detects other glycolipids.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000145:classification; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D015478:Antigens, Heterophile / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D005577:Forssman Antigen / Q000276:immunology*; D005915:Globosides / Q000276:immunology*; D006028:Glycosphingolipids / Q000276:immunology*; D006241:Haptens / Q000276:immunology*; D007136:Immunoglobulins / Q000145:classification; D008325:Mammary Neoplasms, Experimental / Q000276:immunology; D051381:Rats
D000911:Antibodies, Monoclonal; D015478:Antigens, Heterophile; D000939:Epitopes; D005915:Globosides; D006028:Glycosphingolipids; D006241:Haptens; D007136:Immunoglobulins; C010163:Forssman glycolipid; D005577:Forssman Antigen