title: Voltage clamp simulations for multifiber bundles in a double sucrose gap: cable complications.
abstract: A theoretical model is presented for voltage clamp of a bundle of cylindrical excitable cells in a double sucrose gap. The preparation in the test node is represented by a single one-dimensional cable (length/diameter ratio approximately) with standard Hodgkin-Huxley kinetics for transmembrane Na current. Imperfections of voltage control due to internal (longitudinal) resistivity and external (radial) resistance in series to the membrane are analysed. The electrical behavior of a fiber is described by the cable equation with appropriate boundary conditions and subsidiary equations reflecting the membrane characteristics. Membrane voltage and current distribution in response to a step command was obtained by numerical integration. The results are described in two papers. The present paper deals with the effect of internal resistivity with the external resistance being neglected. The closed loop response of a fiber displays a strong tendency to oscillate. To stabilize the system a phase lead was inserted and the gain of the control amplifier was reduced. Conditions for stability were examined by Nyquist analysis. When the Na system was activated by a command pulse below ENa, a voltage gradient developed between a depolarization (relative to the command signal) at the end where voltage was monitored and a hyperpolarization at the site of current injection. In spite of a poor voltage control the total measured current appeared to have a smooth transient. With large voltage gradients a small, second inward current was seen. At a low (high) Na conductance maximum peak inward current was larger (smaller) that the current expected from ideal space clamping.
mesh_terms: D000818:Animals; D003198:Computer Simulation; D004553:Electric Conductivity; D004594:Electrophysiology; D004868:Equipment Failure; D007473:Ion Channels / Q000502:physiology; D008564:Membrane Potentials; D008962:Models, Theoretical; D009130:Muscle, Smooth / Q000502:physiology*; D011817:Rabbits; D013395:Sucrose / Q000502:physiology*
chemical_list: D007473:Ion Channels; D013395:Sucrose