Transcriptional terminators in the caa-cal operon and cai gene.
We have analysed by S1 nuclease mapping the in vivo termination sites of transcription of the caa-cal operon and cai gene. The termination region for caa mRNA (T1A terminator) features characteristics of a rho-independent terminator. This terminator is a convergent transcription terminator, its complementary secondary structure being present at the 3'-end of cai mRNA. The caa-cal mRNA terminator (T2A terminator) has a stable potential secondary structure and shows homology with rho-dependent terminators. In vitro transcription of caa-cal operon demonstrated that the two terminators T1A and T2A are efficient. The 3'-ends of the mRNAs which end at T1A and T2A were analysed by S1 mapping with total RNA purified from a mutant strain deficient in exoribonuclease activities, in particular RNase II. The results suggest that the potential secondary structures of T1A and T2A are sufficiently stable to prevent 3'-end degradation by RNase II. On the other hand, the T2A terminator should be efficient enough to stop transcription through the downstream DNA region involved in pColA replication.
D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping; D009876:Operon; D012329:RNA, Bacterial / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics*; D014158:Transcription, Genetic
D012329:RNA, Bacterial; D012333:RNA, Messenger; D004262:DNA Restriction Enzymes