Regulated expression of tumor-specific and MHC class I antigens in an ultraviolet radiation-induced murine skin cancer.
Previous studies have shown that the ultraviolet (UV) radiation-induced tumor UV-1591 expresses a novel class I antigen that functions as a tumor-specific transplantation antigen (TSTA). The purpose of this study was to determine whether expression of novel class I TSTA and expression of endogenous class I antigens in UV-1591 are regulated independently or coordinately. Expression of both TSTA and H-2Kk antigens increased simultaneously after treatment of UV-1591 cells with either 5-azadeoxycytidine (5-azaC) or interferon-gamma (IFN-gamma). Furthermore, the kinetics and pattern of increased expression by 5-azaC and IFN-gamma were identical for both TSTA and H-2Kk antigens. The increased expression of TSTA and class I antigens induced by 5-azaC treatment was constitutive and did not require the continued presence of the drug. In contrast, the increased expression after IFN-gamma treatment was transient and required the continued presence of IFN-gamma. Neither 5-azaC nor IFN-gamma caused induction of class II antigens in UV-1591 tumor cells. These results indicate that expression of TSTA and class I antigens in UV-1591 tumor is coordinately regulated, whereas expression of class I and class II antigens is independently regulated.
D000818:Animals; D000951:Antigens, Neoplasm / Q000096:biosynthesis* / Q000235:genetics; D001374:Azacitidine / Q000031:analogs & derivatives / Q000494:pharmacology; D000077209:Decitabine; D005786:Gene Expression Regulation / Q000187:drug effects; D006183:H-2 Antigens / Q000096:biosynthesis* / Q000235:genetics; D006649:Histocompatibility Antigens / Q000096:biosynthesis* / Q000235:genetics; D007371:Interferon-gamma / Q000494:pharmacology; D008745:Methylation; D051379:Mice; D009381:Neoplasms, Radiation-Induced / Q000276:immunology*; D012878:Skin Neoplasms / Q000276:immunology*; D014466:Ultraviolet Rays
D000951:Antigens, Neoplasm; D006183:H-2 Antigens; D006649:Histocompatibility Antigens; C029237:tumor-associated transplantation antigen; D000077209:Decitabine; D007371:Interferon-gamma; D001374:Azacitidine