title: Cis-acting sequences which regulate expression of the Sgs-4 glue protein gene of Drosophila.
abstract: The Sgs-4 glue protein gene of Drosophila is expressed only in third-instar larval salivary glands. Previous work suggests that a regulatory region lies 5' and remote to the gene, as indicated by a region of tissue-specific DNase I hypersensitivity and by underproducing mutants with DNA lesions in the hypersensitive region. Here we demonstrate by germ line transformation of cloned fragments containing Sgs-4 that the sequences between 840 bp 5' and 130 bp 3' to the gene are sufficient for Sgs-4 activity. When 5' sequence was removed to -392, activity was eliminated, thereby verifying the existence of essential sequences far upstream. Fragments that are active include, in addition to the capacity for normal levels of expression, three other cis-acting regulatory activities: developmental timing, tissue specificity, and dosage compensation. In contrast, the fragments tested did not specify formation of the puff with which Sgs-4 is normally associated. As shown by chromosomal rearrangements, the region required for puffing is limited to 16-19 kb surrounding the gene.
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D005796:Genes; D008040:Genetic Linkage; D005967:Glue Proteins, Drosophila; D008297:Male; D010957:Plasmids; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012471:Salivary Proteins and Peptides / Q000235:genetics*; D014158:Transcription, Genetic; D014161:Transduction, Genetic; D014960:X Chromosome
chemical_list: D005967:Glue Proteins, Drosophila; D012471:Salivary Proteins and Peptides; D012313:RNA