title: Myelin-associated glycoprotein and related glycoconjugates in developing cat peripheral nerve: a correlative biochemical and morphometric study.
abstract: The expression and accumulation of the myelin-associated glycoprotein (MAG) and other glycoconjugates have been studied during myelination in the developing cat peripheral nervous system. The glycoconjugates studied have in common a similar carbohydrate determinant which is bound by many antibodies, including the mouse monoclonal antibody HNK-1, and human IgM paraproteins from patients with neuropathy. In addition to MAG, the reactive glycoconjugates include a 60-kilodalton (kD) glycoprotein and a group of 20-26 kD glycoproteins, as well as a group of recently identified acidic glycolipids, the major one of which is sulfate-3-glucuronyl paragloboside (SGPG). The accumulation of these glycoproteins and glycolipids is compared with the established myelin proteins P0, P1, and P2 and with morphometric indices of myelin volume and axonal perimeter. The study demonstrates that MAG appears and accumulates very early during myelination, being present at 15% of the maximum level prior to the appearance of P0, and at 80% of the maximum level when P0 is at 30% of its maximum level. In the adult, the level of MAG falls to 60% maximum. The 60 kD and 20-26 kD glycoproteins accumulate at the same time as or later than P0, suggesting that they are either compact myelin proteins or in membranes closely associated with compact myelin. SGPG accumulates with P0 early in myelination, but falls to 60% of maximum in the adult. By comparing biochemical and morphometric data, we demonstrate that P0 and other compact myelin proteins accumulate synchronously with the increase in myelin area. MAG accumulation, however, is closely related to changes in axonal perimeter, consistent with a predominant localization of MAG to the periaxonal membranes in the peripheral nervous system.
mesh_terms: D000375:Aging / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal; D002415:Cats; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D006017:Glycolipids / Q000378:metabolism; D006023:Glycoproteins / Q000276:immunology / Q000378:metabolism*; D007118:Immunoassay; D004676:Myelin Basic Protein / Q000378:metabolism; D018993:Myelin P0 Protein; D018996:Myelin P2 Protein; D009185:Myelin Proteins / Q000276:immunology / Q000378:metabolism*; D009186:Myelin Sheath / Q000502:physiology*; D018992:Myelin-Associated Glycoprotein; D010525:Peripheral Nerves / Q000196:embryology / Q000254:growth & development* / Q000378:metabolism; D012584:Sciatic Nerve / Q000033:anatomy & histology / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D006017:Glycolipids; D006023:Glycoproteins; D004676:Myelin Basic Protein; D018993:Myelin P0 Protein; D018996:Myelin P2 Protein; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; C031633:myelin basic protein P1