Cationic heme undecapeptide as stain for detecting glycosaminoglycans on cellulose acetate strips.
A rapid and sensitive method has been developed for detecting glycosaminoglycans on cellulose acetate strips. The method is based on the electrostatic interactions between glycosaminoglycans and cationic heme undecapeptide. The complexes are identified by peroxidatic reaction using 3,3'-diaminobenzidine as H2 donor. The detection limit is 2.5-10 ng for sulfated glycosaminoglycans and 60 ng for hyaluronic acid. The sensitivity of this method is higher than of those using Alcian blue or toluidine blue (500 ng).
D000818:Animals; D002417:Cattle; D004588:Electrophoresis, Cellulose Acetate; D006025:Glycosaminoglycans / Q000032:analysis*; D006418:Heme / Q000031:analogs & derivatives; D006427:Hemin; D006801:Humans; D009842:Oligopeptides; D010544:Peroxidases; D013194:Staining and Labeling; D013552:Swine
D006025:Glycosaminoglycans; D009842:Oligopeptides; C013994:ferri-heme undecapeptide; D006418:Heme; D006427:Hemin; D010544:Peroxidases