A nonendocytotic mechanism for the selective uptake of high density lipoprotein-associated cholesterol esters.
We have previously described in rats the selective uptake of HDL-associated cholesterol esters (traced by [3H]cholesteryl oleyl ether) in excess of the uptake of HDL-associated apoA-I. In the present studies we show that the mechanism also exists in cultured cells of human and mouse origin as well. This selective uptake represents a net uptake of cholesterol esters and not an isotope exchange, as shown by mass flux studies in adrenal cells. Inhibitors of receptor recycling, chloroquine, monensin, and colchicine, inhibited uptake of apoA-I from HDL by Hep G-2 human hepatoma cells to about the same extent as a reference protein, asialofetuin, but inhibited uptake of the cholesteryl ether tracer much less. Levels of NaN3 which effectively inhibited sucrose pinocytosis inhibited uptake of apoA-I to about the same extent but did not inhibit uptake of the cholesteryl ether at all. Thus, not only receptor recycling, but endocytosis as well, appears not to be involved in selective uptake. This conclusion was supported by studies in which synthetic HDL particles were made to contain two neutral lipid core tracers; one of them, the [3H]cholesteryl ether previously used, was selectively taken up, whereas the other, [14C]sucrose octaoleate, was excluded from selective uptake. Thus, selective uptake cannot involve endocytosis of the entire lipid core, but may involve other specific transfer mechanisms.
D000306:Adrenal Cortex Neoplasms / Q000378:metabolism; D000818:Animals; D016632:Apolipoprotein A-I; D001054:Apolipoproteins A / Q000378:metabolism; D001212:Asialoglycoproteins; D001386:Azides / Q000494:pharmacology; D002478:Cells, Cultured; D002738:Chloroquine / Q000494:pharmacology; D002784:Cholesterol / Q000031:analogs & derivatives / Q000378:metabolism; D002788:Cholesterol Esters / Q000378:metabolism*; D003078:Colchicine / Q000494:pharmacology; D060748:Fetuins; D005347:Fibroblasts / Q000378:metabolism; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D008075:Lipoproteins, HDL / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000494:pharmacology; D008113:Liver Neoplasms / Q000378:metabolism; D051379:Mice; D008985:Monensin / Q000494:pharmacology; D010873:Pinocytosis; D051381:Rats; D019810:Sodium Azide; D000509:alpha-Fetoproteins / Q000378:metabolism
D016632:Apolipoprotein A-I; D001054:Apolipoproteins A; D001212:Asialoglycoproteins; D001386:Azides; D002788:Cholesterol Esters; D060748:Fetuins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D000509:alpha-Fetoproteins; C017029:asialofetuin; C023511:cholesteryl oleyl ether; D002738:Chloroquine; D006493:Heparin; D008985:Monensin; D019810:Sodium Azide; D002784:Cholesterol; D003078:Colchicine