title: Nucleotide sequence of a bovine protamine cDNA.
abstract: The nucleotide sequence of a 441-base cDNA encoding the bovine protamine has been determined. This insert, isolated from a bovine spermatid-specific cDNA library, encodes a polypeptide of 50 amino acids of which 26 are arginine, 7 are cysteine, and 2 are tyrosine. The insert contains the complete 3'-noncoding region of 150 bases and most of the 5'-noncoding region. The predicted amino-acid sequence of bovine protamine is about 96% homologous to ram protamine, 76% to boar protamine, 64% to mouse protamine 1 and 52% to human protamine 1 and contains the central, highly basic domain of four arginine clusters found in the trout protamines. Our results show that bovine protamine is 50 amino-acid residues in length and not 47 residues as previously published (Coelingh, J.P. et al. (1972) Biochim. Biophys. Acta 285, 1-14).
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D004247:DNA / Q000032:analysis*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D011479:Protamines / Q000096:biosynthesis*; D012313:RNA / Q000302:isolation & purification; D051381:Rats; D012756:Sheep; D013045:Species Specificity; D013087:Spermatids / Q000032:analysis; D013552:Swine
chemical_list: D011479:Protamines; D012313:RNA; D004247:DNA