[Use of gamma-globulins from the peritoneal fluid of cats with infectious peritonitis for the immunofluorescence demonstration of the TGE antigen].
Peritoneal exudate of a cat affected with feline infectious peritonitis (FIP) was used to isolate an IgG-globulin, employing affinity chromatography, and conjugate it with fluorescein isothiocyanate. The conjugate was tested on cross sections of the small intestine of a pig infected with strain Miller-3 of the transmissive gastroenteritis (TGE) virus, simultaneously with an anti-TGE conjugate of an SPF pig. Identical results were obtained with both conjugates, however, the anti-FIP conjugate had a several-times higher titer. Discussed are the possibilities and advantages of using the anti-FIP conjugate in the immunofluorescence demonstration of the TGE virus antigen.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D001202:Ascitic Fluid / Q000276:immunology*; D002371:Cat Diseases / Q000276:immunology*; D002415:Cats; D003332:Coronaviridae / Q000276:immunology*; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D005456:Fluorescent Dyes; D005761:Gastroenteritis, Transmissible, of Swine / Q000276:immunology; D007074:Immunoglobulin G / Q000302:isolation & purification; D007421:Intestine, Small / Q000276:immunology; D010538:Peritonitis / Q000276:immunology / Q000662:veterinary*; D013552:Swine; D013861:Thiocyanates; D005760:Transmissible gastroenteritis virus / Q000276:immunology*; D005719:gamma-Globulins / Q000302:isolation & purification
D000956:Antigens, Viral; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D013861:Thiocyanates; D005719:gamma-Globulins; D016650:Fluorescein-5-isothiocyanate
Izpolzuvane na gama-globulini ot peritonealna technost ot kotki, bolni ot infektsiozen peritonit (FIP), za imunofluorestsentno pozitivirane na TGE-antigen.