Metabolism of androgens in human benign prostatic hyperplasia: aromatase and its inhibition.
As an extension of our studies on androgen metabolism in epithelium and stroma of human benign prostatic hyperplasia (BPH) tissue our attempts to demonstrate the presence of aromatase are described. Additionally, the question is raised whether the aromatase inhibitor 17 alpha-oxa-D-homoandrosta-1.4-diene-3.17-dione (testolactone) might also act by inhibition of 17 beta-hydroxysteroid dehydrogenase (17 beta-HSDH). In vitro metabolism and inhibition were analyzed by TLC. The main results were: (1) Two aromatase assays (estrone formation and tritium release) were tested with placenta microsomes. Identical results were obtained (Km = 43 +/- 7 nmol/l n = 5; Vmax = 100 resulted in recovery of the aromatase activity added. (3) In BPH tissue alone, formation of estrone from androstenedione could not be detected (less than 7 x 10(-17) mol/min per mg protein, n = 8). (4) 4-Hydroxyandrostenedione inhibited placental aromatase (Ki = 37 nmol/l) distinctly better than 17 beta-HSDH from human BPH (Ki = 18 mumol/l), whereas the Ki values for testolactone (3.7 and 29 mumol/l, respectively) were more similar. It is concluded that aromatization of androgens is not an important pathway in BPH tissue. An alternative mode of action of testolactone by inhibition of 17 beta-HSDH is discussed.
D015067:17-Hydroxysteroid Dehydrogenases / Q000037:antagonists & inhibitors; D000728:Androgens / Q000378:metabolism*; D000735:Androstenedione / Q000031:analogs & derivatives / Q000494:pharmacology; D001141:Aromatase / Q000378:metabolism*; D047072:Aromatase Inhibitors; D004970:Estrone / Q000096:biosynthesis; D006801:Humans; D008297:Male; D008861:Microsomes / Q000201:enzymology; D010920:Placenta / Q000201:enzymology; D011467:Prostate / Q000201:enzymology*; D011470:Prostatic Hyperplasia / Q000378:metabolism*; D013738:Testolactone / Q000494:pharmacology
D000728:Androgens; D047072:Aromatase Inhibitors; D004970:Estrone; D000735:Androstenedione; D013738:Testolactone; D015067:17-Hydroxysteroid Dehydrogenases; D001141:Aromatase; C014594:formestane