Comparison of Plasmagel with LeucoPREP-Macrodex methods for separation of leukocytes for virus isolation.
Plasmagel (Cellular Products, Inc., Buffalo, NY), which can separate both polymorphonuclear leukocytes (PMN) and mononuclear cells from other blood components, and LeucoPREP (Becton Dickinson Immunocytometry Systems, Mountain View, CA), which can separate mononuclear cells from other blood components, were used to harvest leukocytes from whole blood for the purpose of virus isolation. Macrodex was combined with the later, in a second step, for recovery of PMN. Of 90 peripheral blood specimens examined, cytomegalovirus was recovered from 10: in six by both methods, in three from Plasmagel prepared cells only, and in one from cells from the LeucoPREP-Macrodex preparation only. Total leukocyte counts, differential counts, and leukocyte viability did not differ significantly for the two methods. Plasmagel provided an efficient, inexpensive means of harvesting leukocytes from whole blood for virus isolation.
D002469:Cell Separation / Q000379:methods*; D003587:Cytomegalovirus / Q000302:isolation & purification; D003911:Dextrans; D005780:Gelatin / Q000031:analogs & derivatives; D006801:Humans; D007962:Leukocytes / Q000382:microbiology*; D007963:Leukocytes, Mononuclear / Q000382:microbiology; D009504:Neutrophils / Q000382:microbiology; D014780:Viruses / Q000302:isolation & purification*
D003911:Dextrans; C025915:plasmagel; D005780:Gelatin