Increased turnover of vaccinia virus-specific immediate early RNAs in interferon-treated chick embryo fibroblasts.
The synthesis and steady state level of immediate early vaccinia virus-specific RNAs in interferon-treated chick embryo fibroblasts were determined by blot hybridization analysis using the cloned restriction endonuclease fragment pEJ 18 containing the gene of vaccinia virus WR-specific DNA polymerase as a probe. Even though early vaccinia virus WR RNA was still synthesized, accumulation of immediate early viral RNAs was strongly inhibited. Accumulation of beta-actin RNA was not affected. This indicated an enhanced degradation of vaccinia virus WR-specific early RNAs in interferon-treated chick embryo fibroblasts. This notion was supported by Northern blot analysis which revealed degradation of residual RNA of vaccinia virus WR-specific DNA polymerase. In contrast to interferon-treated mouse L 929 cells, ribosomal RNA is not degraded in interferon-treated vaccinia WR-infected chick embryo fibroblasts.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D007372:Interferons / Q000494:pharmacology*; D007739:L Cells / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D051379:Mice; D012367:RNA, Viral / Q000378:metabolism*; D014616:Vaccinia virus / Q000378:metabolism*
D012367:RNA, Viral; D007372:Interferons; D004259:DNA-Directed DNA Polymerase