Effect of isoprinosine on sialylation of interleukin-2.
The effects of Isoprinosine (ISO) on interleukin-2 (IL-2) production by human peripheral blood mononuclear cells (PBMC) were investigated. Treatment (of human PBMC) with ISO enhanced IL-2 production by PBMC from 7 of 10 normal individuals. However, no augmentation of IL-2 production was observed when cultures of HUT-78 cells, a human leukemic T cell line, were treated with ISO. IL-2 purified from supernatants of human PBMC treated with ISO exhibited pI values of 5.5 and 6.4. IL-2 prepared from untreated PBMC exhibited a single pI value of 8.2. The pI value of IL-2 prepared from ISO-treated PBMC shifted to 8.2 after treatment with neuraminidase, demonstrating that the IL-2 molecules isolated from ISO-treated PBMC possessed sialic acid. The pI values of the IL-2 isolated from ISO-treated and untreated HUT-78 culture supernatants were identical (pI = 7.8) and were not modified by neuraminidase treatment. These results suggest that the increase in IL-2 production following treatment of PBMC with ISO may be mediated through the activation of a distinct subset of IL-2 producing cells. Furthermore, the sialylation of IL-2 may be of physiologic and immunopharmacologic importance.
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000328:Adult; D002460:Cell Line; D002478:Cells, Cultured; D002846:Chromatography, Affinity / Q000379:methods; D003470:Culture Media / Q000032:analysis; D006801:Humans; D007288:Inosine / Q000031:analogs & derivatives*; D007542:Inosine Pranobex / Q000494:pharmacology*; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000302:isolation & purification; D007525:Isoelectric Focusing; D037102:Lectins / Q000494:pharmacology; D007938:Leukemia / Q000097:blood; D008214:Lymphocytes / Q000145:classification / Q000378:metabolism; D009439:Neuraminidase / Q000494:pharmacology; D012794:Sialic Acids / Q000378:metabolism*
D000276:Adjuvants, Immunologic; D003470:Culture Media; D007376:Interleukin-2; D037102:Lectins; D012794:Sialic Acids; D007288:Inosine; D009439:Neuraminidase; D007542:Inosine Pranobex