Immunogenicity of polyethylene glycol-modified superoxide dismutase and catalase.
Modification of proteins with polyethylene glycol (PEG) has been shown to result in a decrease in immunogenicity. Superoxide dismutase (SOD) and catalase were modified with PEG and used to immunize mice. Antibody titers against the antigens were determined by ELISA. Mice immunized with PEG-SOD had antibody titers 0.03%-0.07% of that seen in mice with SOD, while mice immunized with PEG-catalase developed titers 0.02%-0.09% of that seen in mice with catalase. The modified enzymes retained the ability to react with preformed antibodies to the unmodified antigens. Antibodies to SOD reacted equally well with the PEG-SOD or SOD antigen. Antibodies to catalase reacted to PEG-catalase but at only 0.02% of the reaction with catalase antigen. In reciprocal studies, antisera against the PEG-proteins failed to react to an appreciable level with the corresponding unmodified protein. Modification with PEG resulted in a decrease in immunogenicity of both SOD and catalase.
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D002374:Catalase / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005260:Female; D051379:Mice; D011092:Polyethylene Glycols / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000276:immunology*
D000906:Antibodies; D000939:Epitopes; D011092:Polyethylene Glycols; D002374:Catalase; D013482:Superoxide Dismutase