[Synthetic peptides simulating the protective epitopes of VP1 protein of foot-and-mouth disease virus type O and A].
In a search of novel approaches to cattle protection from foot-and-mouth disease we have prepared a series of peptides from the major antigenic region 130-160 of the VP1 protein. The 144-159 peptide as well as 141-152, 141-148, 148-159 segments (strain O1K) were inactive in all in vitro and in vivo experiments on virus inhibiting. On the other band, synthetic 136-152, 136-148 O1K sequences as well as 131-149, 140-149 A22 sequences afforded 50 to 100% protection, both in the free state and conjugated with keyhole limpet hemocyanin. Therefore the 136-145 region should be considered as an essential part of the major sequential epitope, necessary for full-scale antiviral immune response. We also believe that the 136-152 segment is so far the smallest peptide capable of eliciting virus neutralizing antibodies and antiviral protection without conjugation with a high-molecular carrier.
D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000276:immunology*; D005537:Aphthovirus / Q000276:immunology*; D000939:Epitopes / Q000032:analysis*; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*; D015678:Viral Structural Proteins
D000956:Antigens, Viral; D000939:Epitopes; D010455:Peptides; D014764:Viral Proteins; D015678:Viral Structural Proteins
Modelirovanie s pomoshch'iu sinteticheskikh peptidov protektivnykh épitopov belka VP1 virusa iashchura serotipov O i A.