[Immunomodulating activity of RNA mononucleotides].
The immunological action of RNA mononucleotides was studied in animal experiments. The most pronounced activation of macrophagal glycolysis urea cycle, oxidative phosphorylation, lysosomal hydrolases was induced by uridine 5'-monophosphate (5'-UMP) and guanosine 5'-monophosphate (5'-GMP); 5'-GMP also induced the maximum increase of the expression of FC gamma receptors. 5'-UMP ensured cell activation comparable with the total action of all mononucleotides. 5'-UMP and 5'-GMP, used in combination, produced the highest stimulating effect on macrophages, and the addition of low-active adenosine 5'-monophosphate (5'-AMP) to active 5'-UMP did not decrease the stimulating potency of the latter. The stimulating activity of sodium nucleinate exceeded that of all mononucleotides and their combinations. 5'-GMP and 5'-AMP induced the maximum activation of oxygen metabolism, evaluated by chemiluminescence, while 5'-UMP and cytidine 5'-monophosphate (5'CMP) proved to be inactive. The shift of the phosphate group to the third carbon atom or the production of the oligonucleotide 5'-UMP consisting of 5-15 nucleotides resulted in the appearance of the capacity for stimulating oxygen metabolism in macrophages. Their in vitro cultivation with 5'-GMP and 5'-AMP induced the maximum increase of cell spreading in comparison with other mononucleotides, while the maximum increase of phagocytosis was ensured only by 5'-UMP and 5'-GMP.2+ 5'-UMP and 5'-GMP enhanced nonospecific resistance to Salmonella typhi infection, and sodium nucleinate, to Pseudomonas pseudomallei and Pseudomonas mallei infections.
D000249:Adenosine Monophosphate / Q000494:pharmacology; D000276:Adjuvants, Immunologic; D000818:Animals; D006224:Cricetinae; D003568:Cytidine Monophosphate / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006157:Guanosine Monophosphate / Q000494:pharmacology; D007114:Immunization; D008163:Luminescent Measurements; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology / Q000276:immunology; D008554:Melioidosis / Q000276:immunology; D008647:Mesocricetus; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009711:Nucleotides / Q000494:pharmacology*; D010587:Phagocytosis / Q000187:drug effects; D012313:RNA / Q000494:pharmacology*; D011961:Receptors, Fc / Q000187:drug effects / Q000276:immunology; D013329:Structure-Activity Relationship; D014435:Typhoid Fever / Q000276:immunology; D014542:Uridine Monophosphate / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D009711:Nucleotides; D011961:Receptors, Fc; D000249:Adenosine Monophosphate; D012313:RNA; D006157:Guanosine Monophosphate; D014542:Uridine Monophosphate; D003568:Cytidine Monophosphate
Immunomoduliruiushchaia aktivnost' mononukleotidov RNK.