The NADP-dependent trifunctional methylenetetrahydrofolate dehydrogenase purified from mouse liver is immunologically distinct from the mouse NAD-dependent [corrected] bifunctional enzyme.
Methylenetetrahydrofolate dehydrogenase - methenyltetrahydrofolate cyclohydrolase - formyltetrahydrofolate synthetase was purified to homogeneity from mouse liver, taking advantage of its very high affinity for 2',5'-ADP-Sepharose. Antibodies raised to this trifunctional enzyme and to the bifunctional NAD-dependent dehydrogenase-cyclohydrolase from mouse Ehrlich ascites tumour cells were found not to cross-react with the purified proteins on Western blots. Each of these polyclonal antibodies detects the appropriate protein in extracts of Ehrlich ascites tumour cells after sodium dodecyl sulfate - polyacrylamide gel electrophoresis and electrophoretic transfer of the proteins to nitrocellulose. The procedure has also been used to obtain a purified preparation of the trifunctional enzyme from human liver obtained at autopsy.
D000619:Aminohydrolases / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D000939:Epitopes / Q000032:analysis; D005574:Formate-Tetrahydrofolate Ligase / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D007106:Immune Sera; D007527:Isoenzymes / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008754:Methylenetetrahydrofolate Dehydrogenase (NADP) / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D051379:Mice; D009097:Multienzyme Complexes / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D009249:NADP / Q000378:metabolism*; D010088:Oxidoreductases / Q000302:isolation & purification*; D013379:Substrate Specificity
D000939:Epitopes; D007106:Immune Sera; D007527:Isoenzymes; D009097:Multienzyme Complexes; C055342:methylene tetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase; D009249:NADP; C021836:formyl-methenyl-methylenetetrahydrofolate synthetase; D010088:Oxidoreductases; D008754:Methylenetetrahydrofolate Dehydrogenase (NADP); D000619:Aminohydrolases; D005574:Formate-Tetrahydrofolate Ligase