Angiotensin II increases cytosolic calcium and stimulates catecholamine release in cultured bovine adrenomedullary cells.
In bovine adrenomedullary cells in primary culture, angiotensin II (AII) elicited virtually immediate, dose-related increments in cytosolic calcium [( Ca++]i) measured by the Quin 2 technique and stimulated approximately proportional secretion of norepinephrine, epinephrine, and dopamine measured by liquid chromatography with electrochemical detection. Peak responses of [Ca++]i to AII were similar to peak responses to nicotine or KCl. Pre-treatment with verapamil or washing the cells in calcium-free medium attenuated the stimulatory effect of AII on [Ca++]i. Pre-treatment with nicotine, which temporarily inactivates cholinergic receptor-activated calcium channels, did not affect [Ca++]i responses to AII. The results indicate functional effects of AII on cultured chromaffin cells. The mechanism of cellular activation by AII appears to include increases in [Ca++]i due to opening of membrane calcium channels which may be unrelated to cholinergic receptor-operated calcium channels.
D000313:Adrenal Medulla / Q000187:drug effects / Q000378:metabolism*; D000634:Aminoquinolines; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002395:Catecholamines / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism; D004837:Epinephrine / Q000378:metabolism; D005456:Fluorescent Dyes; D007473:Ion Channels / Q000502:physiology; D009538:Nicotine / Q000494:pharmacology; D009638:Norepinephrine / Q000378:metabolism; D011189:Potassium Chloride / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D014700:Verapamil / Q000494:pharmacology
D000634:Aminoquinolines; D002395:Catecholamines; D005456:Fluorescent Dyes; D007473:Ion Channels; D000804:Angiotensin II; D011189:Potassium Chloride; D009538:Nicotine; D014700:Verapamil; C035739:Quin2; D002118:Calcium; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine