Effect of heparin-binding growth factors on monovalent cation transport in Balb/C 3T3 cells.
The changes in monovalent cation fluxes induced by class 1 and class 2 heparin-binding growth factors purified from bovine brain were studied in confluent quiescent Balb/C 3T3 cells. Both classes of HBGF's rapidly stimulate the activity of the Na+-K+ pump in a concentration-dependent manner that parallels their mitogenic activity. This effect requires the presence of external Na+ and is abolished by 1 mM amiloride. As with other growth factors, HBGF's increase the activity of the Na+-K+ pump by increasing Na+ influx into the cells via the amiloride-sensitive Na+-H+ exchange. These results indicate that rapid activation of cation transport is an early cellular response that follows binding of HBGF's to specific receptors on the surface of target cells.
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D005346:Fibroblast Growth Factors; D006133:Growth Substances / Q000145:classification / Q000494:pharmacology*; D006493:Heparin / Q000145:classification / Q000494:pharmacology*; D007473:Ion Channels / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D012413:Rubidium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism
D006133:Growth Substances; D007473:Ion Channels; D010042:Ouabain; D005346:Fibroblast Growth Factors; D006493:Heparin; D012964:Sodium; D012413:Rubidium; D011188:Potassium