title: Location of the regions recognized by five commercial antibodies on the tubulin molecule.
abstract: Through limited proteolysis of the tubulin molecule with trypsin, chymotrypsin, subtilisin, or pronase we have mapped the regions recognized by five commercial antibodies. Two of them recognized a sequence between amino acids 340 and 400 near the C terminal of the alpha or beta subunits, one recognized a sequence between amino acids 120 and 150 present in both subunits, and another one probably recognized a conformational epitope. Simultaneously we have confirmed the results obtained for other antibodies which recognized a region previously mapped on the tubulin molecule.
mesh_terms: D000595:Amino Acid Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis*; D006868:Hydrolysis; D007120:Immunochemistry; D010446:Peptide Fragments / Q000276:immunology; D010447:Peptide Hydrolases; D014404:Tubulin / Q000276:immunology*
chemical_list: D000939:Epitopes; D010446:Peptide Fragments; D014404:Tubulin; D010447:Peptide Hydrolases