[Receptor-mediated endocytosis of alpha 2-macroglobulin in living intact 3T3 cells].
Internalization process of the fluoresceinisothiocyanate-labeled alpha 2-macroglobulin (MG-F) in Swiss 3T3 cells has been studied. The presence of internalized MG-F, formation of endocytotic vesicles and endosomal pH value were estimated visually on living monolayer cells with TV microscope, on the base of supervidicon LI-702. The use of the TV method allowed to obtain a series of photographs of cell images without any significant dye fading. A brief characteristics of microprocess control system is presented. The influence of various chemical agents on MG-F internalization and endosomal pH value was investigated. It was shown that dansylcadaverine (150-200 microM) and monensin (10-20 microM) inhibited MG-F internalization. Methylamine, chloroquine and monensin quickly enhanced the endosomal pH value, while other amines did not, for example dansylcadaverine.
D000818:Animals; D002478:Cells, Cultured; D004705:Endocytosis; D011992:Endosomes / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006801:Humans; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008856:Microscopy, Fluorescence / Q000295:instrumentation; D013690:Television / Q000295:instrumentation; D013861:Thiocyanates; D000511:alpha-Macroglobulins / Q000378:metabolism*
D005452:Fluoresceins; D005456:Fluorescent Dyes; D013861:Thiocyanates; D000511:alpha-Macroglobulins; D016650:Fluorescein-5-isothiocyanate
Issledovanie retseptor-oposredovannogo éndotsitoza al'fa 2-makroglobulina v zhivykh intaktnykh kletkakh 3T3.