Prevention of experimental autoimmune uveoretinitis by active immunization with autoantigen-specific monoclonal antibodies.
Preimmunization of Lewis rats with anti-S antigen (S-Ag) monoclonal antibodies (mAb) led to protection against experimental autoimmune uveoretinitis (EAU) induced by this retinal autoantigen. High titers of anti-idiotypic (anti-Id) antibodies were raised against three mouse mAb, S2D2 (IgG2b), S6H8 (IgG2a) and S7D6 (IgG1), directed at S-Ag. An almost complete prevention was observed in S2D2 mAb-immunized animals while a partial protection was achieved with S6H8 and S7D6 mAb. No detectable anti-Id antibody nor disease prevention was observed in rats immunized with the mAb S9E2 (IgG2a) which only recognizes bovine and sheep S-Ag, or with control mAb of the same isotypes irrelevant to S-Ag. The mAb treatment did not modify the level of the whole polyclonal antibody response to S-Ag. These results suggest an important role in the pathogenesis of EAU for the epitopes recognized by S2D2-S6H8 and S7D6 in the S-Ag molecule. The success of anti-Id immunization for autoimmune disease suppression may depend on the identification of relevant epitopes.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D000917:Antibody Formation; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology*; D019393:Arrestin; D001324:Autoantigens / Q000276:immunology*; D002417:Cattle; D003429:Cross Reactions; D000939:Epitopes; D005136:Eye Proteins / Q000276:immunology*; D007114:Immunization; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007132:Immunoglobulin Isotypes / Q000276:immunology; D051381:Rats; D012173:Retinitis / Q000276:immunology* / Q000517:prevention & control; D013997:Time Factors; D014605:Uveitis / Q000276:immunology* / Q000517:prevention & control
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000941:Antigens; D019393:Arrestin; D001324:Autoantigens; D000939:Epitopes; D005136:Eye Proteins; D007130:Immunoglobulin Idiotypes; D007132:Immunoglobulin Isotypes