The specificities of polyclonal and monoclonal anti-idiotypes to anti-alpha(1----6)dextrans; possible correlations of idiotype with amino acid sequence.
The specificities of polyclonal and monoclonal anti-idiotypes to three anti-alpha(1----6)dextrans-10.16.1, QUPC52, and W3129--were examined by competition ELISA. A major idiotype was defined by two polyclonal and two monoclonal anti-idiotypes to 10.16.1, and a polyclonal anti-idiotype to QUPC52. Another monoclonal anti-idiotype to 10.16.1 defines a non-overlapping determinant. One monoclonal anti-idiotype to 10.16.1 and one to W3129 were hapten inhibitable. By comparing amino acid sequences of Id+ and Id- anti-alpha(1----6)dextrans, the major idiotype was assigned to residues in VH CDR3, with a possible contribution from VH CDR2, a conclusion supported by the hapten inhibition results. Both a monoclonal and a previously described polyclonal anti-idiotype to W3129 define a determinant found on only W3129, among the anti-alpha(1----6)dextrans tested.
D000595:Amino Acid Sequence; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions / Q000187:drug effects; D003911:Dextrans / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009844:Oligosaccharides / Q000494:pharmacology; D011817:Rabbits; D013045:Species Specificity
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D003911:Dextrans; D007106:Immune Sera; D007130:Immunoglobulin Idiotypes; D009844:Oligosaccharides; C041999:alpha-1,6-dextran