15N-NMR studies of living systems.
15N-NMR spectroscopy has been applied to the study in vivo of nitrogen uptake during primary and secondary metabolism in Streptomyces parvulus. The nitrogen metabolism of Saccharomyces cerevisiae has been studied in a series of experiments in an effort to elucidate the flow of nitrogen along the various competing pathways as well as its dependence on culture conditions. The low NMR sensitivity of the 15N nucleus required, in spite of high isotopic enrichment, quite long acquisition times (10-20 min). Therefore, an indirect detection method using double quantum 1H-NMR spectroscopy was introduced allowing the selective detection of 15N-bound protons with excellent S/N-ratio in less than a minute.
D009682:Magnetic Resonance Spectroscopy; D009584:Nitrogen / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013302:Streptomyces / Q000378:metabolism
D009584:Nitrogen