Monoclonal antibodies of predetermined specificity for fibrin: a rational approach to monoclonal antibody production.
Three monoclonal antibodies (MAbs) (55D10, 59D8, and 64C5) have been produced by an immunization strategy that uses a synthetic heptapeptide from the amino terminus of the beta-chain in human fibrin. These MAbs bind to human fibrin in the presence of 4 mg/ml human fibrinogen in solid-phase radioimmunoassay. Ligand specificity studies demonstrate that antifibrin immunoreactivity is completely inhibited by a series of synthetic fibrin-like beta-peptides that bear the position-2 histidyl residue. Although the presence of this residue on the epitope is necessary for its recognition by a fibrin-specific antibody, the presence of the histidyl residue alone is not sufficient for antibody recognition.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D000939:Epitopes / Q000276:immunology; D005260:Female; D005337:Fibrin / Q000276:immunology*; D005340:Fibrinogen / Q000276:immunology; D006639:Histidine; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D010455:Peptides; D006639:Histidine; D005337:Fibrin; D005340:Fibrinogen