title: Spectinomycin resistance at site 1192 in 16S ribosomal RNA of E. coli: an analysis of three mutants.
abstract: Three different single base substitutions were constructed at residue C-1192 in 16S rRNA on a plasmid-coded rrnB operon of E. coli using site-directed mutagenesis. All 3 mutants conferred different levels of resistance to spectinomycin in transformed cells but none affected the growth rate in the absence of the antibiotic. The G-1192 mutant conferred remarkable resistance, permitting growth in 40 mg/ml of spectinomycin.
mesh_terms: D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D009154:Mutation; D010957:Plasmids; D012329:RNA, Bacterial / Q000032:analysis*; D012335:RNA, Ribosomal / Q000032:analysis*; D012336:RNA, Ribosomal, 16S / Q000032:analysis*; D000198:Spectinomycin / Q000494:pharmacology*
chemical_list: D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D000198:Spectinomycin