title: Correlation of pachytene chromomeres and metaphase bands of human chromosomes, and distinctive properties of telomeric regions.
abstract: By means of double staining with DAPI and chromomycin A3, we show that the chromomeres of human pachytene chromosomes are generally DAPI positive and chromomycin negative, like the G- or Q-bands of mitotic chromosomes. Thus we have demonstrated, using an objective technique not based on morphological comparisons, that chromomeres and G-bands are equivalent. However, terminal chromomeres and the ends of mitotic chromosomes, as well as a few other sites, are chromomycin positive and DAPI negative. The ends of human chromosomes appear, therefore, to contain a distinctive class of GC-rich DNA.
mesh_terms: D002865:Chromomycins; D002871:Chromosome Banding; D002877:Chromosomes, Human / Q000648:ultrastructure*; D006801:Humans; D007211:Indoles; D007621:Karyotyping; D008297:Male; D008540:Meiosis; D008938:Mitosis; D013194:Staining and Labeling; D013737:Testis / Q000166:cytology / Q000648:ultrastructure
chemical_list: D002865:Chromomycins; D007211:Indoles; C007293:DAPI