Method of double labelling applied to studies of transcription in isolated rat liver nuclei.
Nonlinear transcription in isolated cell nuclei was observed and elucidated. The method of double labelling of acid insoluble products was applied. Labelled precursors of RNA 14C-CTP and 3H-CTP were introduced separately into incubation media for different times. The parameters of reaction (time of the average template transcription, reinitiation frequency and rate of depolymerization) were optimized by computer. It is suggested that two different types of product are formed, namely 'long' for which the time of synthesis was sometimes longer than 90 min, and 'short' which were sized in less than 5 min. These products were different in respect of both their size and turnover.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D003201:Computers; D007700:Kinetics; D008099:Liver / Q000648:ultrastructure; D008954:Models, Biological; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D014158:Transcription, Genetic
D012313:RNA