Pyrolysis mass spectrometry of bacteria from infected human urine. I. Influence of culturing and antibiotics.
Curie-point pyrolysis mass spectrometry was performed on three bacterial isolates obtained from infected human urine using a novel, direct isolation method. Bacterial samples were analysed directly after isolation, as well as after free growth in broth and after exposure to solutions containing penicillin and gentamicin. The spectra of bacteria directly from urine showed no detectable contamination from urinary constituents. Discriminant analysis indicated genetic strain differences to be greater than the combined variances due to sample preparation or the growth phase. Characteristic biochemical changes related to growth or non-growth were detectable after only 2 h of incubation. The potential usefulness of pyrolysis mass spectrometry techniques for rapid susceptibility testing is discussed.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001437:Bacteriuria / Q000382:microbiology / Q000652:urine*; D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D013058:Mass Spectrometry; D012329:RNA, Bacterial / Q000032:analysis
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D012329:RNA, Bacterial