Localization of muscarinic receptors on peptide-containing neurones of the guinea pig myenteric plexus in tissue culture.
A combined autoradiographic and immunocytochemical procedure has been used to identify neurochemically the subpopulation of cultured myenteric neurones which expresses muscarinic receptors. Antibodies to substance P (SP), [Met]enkephalin (ENK), vasoactive intestinal polypeptide (VIP) and somatostatin (SOM) were used to immunostain cultures that had previously been labelled with the irreversible muscarinic antagonist, [3H]propylbenzilylcholine mustard. Most neurites which displayed SP-like, ENK-like or SOM-like immunoreactivity did not possess muscarinic receptors. In contrast, many VIP-like immunopositive fibres also possessed muscarinic receptors. The identity of the majority of neurones which express muscarinic receptors, that do not contain VIP, remains to be determined.
D000818:Animals; D000831:Animals, Newborn; D004744:Enkephalin, Methionine / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D009197:Myenteric Plexus / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D009924:Organ Culture Techniques; D011976:Receptors, Muscarinic / Q000378:metabolism*; D013004:Somatostatin / Q000032:analysis; D013373:Substance P / Q000032:analysis; D014660:Vasoactive Intestinal Peptide / Q000032:analysis
D011976:Receptors, Muscarinic; D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D013004:Somatostatin; D004744:Enkephalin, Methionine