title: DNA synthesis and interferon release by human peripheral lymphocytes exposed to high potassium medium.
abstract: Lymphocytes have their membrane potential changed during transition from the resting to the active state. On these grounds, we investigated the response of human peripheral lymphocytes to a depolarizing high-potassium (102.7 mM) medium, by assaying cellular incorporation of labeled thymidine and interferon (IFN) release in the culture medium. The greatest effect is evident 3-4 days after preincubation for 120 min in high K+ medium. A temporal correlation has been observed between rate of DNA synthesis and IFN production. The IFN activity is shown to be gamma-type.
mesh_terms: D002478:Cells, Cultured; D004261:DNA Replication; D006801:Humans; D007372:Interferons / Q000097:blood*; D007700:Kinetics; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology*
chemical_list: D007372:Interferons; D011188:Potassium