Expression in bacteria of beta-galactosidase fusion proteins carrying antigenic determinants of the two X gene products of bovine leukemia virus.
A cDNA corresponding to the bovine leukemia virus post-envelope region (X gene) was subcloned into the lambda gt11 expression vector. Two large protein fragments corresponding respectively to the long and short open reading frames of the X region were expressed as beta-galactosidase fusion proteins. These products were specifically recognized by sera from bovine leukemia virus-infected cattle.
D000818:Animals; D002417:Cattle / Q000097:blood; D002460:Cell Line; D000939:Epitopes; D004926:Escherichia coli / Q000201:enzymology*; D005696:Galactosidases / Q000502:physiology*; D001909:Leukemia Virus, Bovine / Q000235:genetics*; D008970:Molecular Weight; D014176:Protein Biosynthesis; D012190:Retroviridae / Q000235:genetics*; D014760:Viral Fusion Proteins / Q000276:immunology*; D001616:beta-Galactosidase / Q000502:physiology*
D000939:Epitopes; D014760:Viral Fusion Proteins; D005696:Galactosidases; D001616:beta-Galactosidase