Molecular characterization of Shaker, a Drosophila gene that encodes a potassium channel.
The Drosophila Shaker (Sh) gene appears to encode a type of voltage-sensitive potassium (K+) channel called the A channel. We have isolated Sh as part of a 350 kb chromosomal walk. The region around Sh contains four identified transcription units. We find that Sh corresponds to a very large transcription unit encompassing a total of about 95 kb of genomic DNA and split by a major 85 kb intron. Sh has multiple hydrophobic domains that have a high probability of being membrane-spanning, consistent with the proposal that it encodes an ion channel.
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004330:Drosophila / Q000235:genetics*; D005786:Gene Expression Regulation; D005796:Genes; D007473:Ion Channels; D014178:Translocation, Genetic
D007473:Ion Channels; D004262:DNA Restriction Enzymes