title: Role of rec genes in SOS-induced inhibition of cell division in Escherichia coli.
abstract: The inhibition of cell division induced by bleomycin (BM) and UV irradiation in the set of rec mutants of E. coli K12 was studied. Data presented in this work indicate that BM treatment requires mainly the RecBC pathway for the induction of cell filamentation. In the recB21 mutant cell filamentation is delayed and reduced compared to the wild type. Cell filamentation is BM-induced with similar kinetics in strains with a proficient RecBC recombination pathway (rec+, recF143 and recN262), as well as in the strain with a fully expressed RecF pathway (recB21recC22sbcB15). Induction is completely abolished in the recB21recF143 double mutant. On the other hand cell filamentation was induced similarly by UV irradiation in all strains with a functional recF gene and in the strain with a fully operative RecF pathway, but it was delayed in the recF143 and recB21recF143 mutants.
mesh_terms: D001761:Bleomycin / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D004260:DNA Repair; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics*; D005798:Genes, Bacterial; D009154:Mutation; D013014:SOS Response, Genetics; D014466:Ultraviolet Rays
chemical_list: D001761:Bleomycin