Muscle biopsy samples for histochemical processing: alterations induced by storage.
Skeletal muscle samples from two healthy dogs were stored in ice at 0 C for up to 30 hours to examine the influence of time on cell morphology and morphometry. Cytochemical and histochemical properties of muscle to 18 hours were not markedly different from fresh frozen tissue. Samples stored to 30 hours were still satisfactory, despite a decline and unevenness in depth of staining. Morphometry from samples stored at 0 C for 6 hours or longer is not recommended, due to the statistically significant increase in diameter (from 21 to 25%) of type I and type II fibers.
D000251:Adenosine Triphosphatases; D000818:Animals; D001391:Azo Compounds; D001706:Biopsy; D004396:Coloring Agents; D004285:Dogs / Q000033:anatomy & histology*; D004801:Eosine Yellowish-(YS); D005615:Freezing; D006416:Hematoxylin; D006651:Histocytochemistry; D006863:Hydrogen-Ion Concentration; D007091:Image Processing, Computer-Assisted; D008739:Methyl Green; D009132:Muscles / Q000033:anatomy & histology / Q000166:cytology*; D009246:NADH Tetrazolium Reductase; D010503:Periodic Acid-Schiff Reaction; D013048:Specimen Handling; D013194:Staining and Labeling; D013696:Temperature; D013997:Time Factors
D001391:Azo Compounds; D004396:Coloring Agents; C017768:trichrome stain; D008739:Methyl Green; D009246:NADH Tetrazolium Reductase; D000251:Adenosine Triphosphatases; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin