A modified periodic acid-thiocarbohydrazide-silver proteinate staining sequence for enhanced contrast and resolution of glycogen depositions by transmission electron microscopy.
Oxidation of araldite-embedded liver sections by 1% w/v aqueous H5IO6 for 15 min and a 5-min reaction of carbonyls with 1% w/v thiocarbohydrazide in 10% v/v acetic acid was employed for subsequent staining of glycogen with silver-proteinate (S-P). The network of branching intracellular glycogen aggregates was revealed by 15-min staining with S-P, whereas 24 hr incubation in S-P was necessary to enhance the contrast of glycogen inclusions. We conclude that the proposed modification of glycogen staining readily affords the means for its localization at a desired level of contrast and resolution.
D000818:Animals; D006003:Glycogen / Q000032:analysis*; D006651:Histocytochemistry; D006834:Hydrazines; D008099:Liver / Q000032:analysis*; D008854:Microscopy, Electron; D010504:Periodic Acid; D051381:Rats; D011919:Rats, Inbred Strains; D012836:Silver Proteins; D013194:Staining and Labeling
D006834:Hydrazines; D012836:Silver Proteins; D010504:Periodic Acid; C011368:thiocarbohydrazide; D006003:Glycogen; C003232:strong silver protein; C009288:metaperiodate