Characterization of epitopes shared by alpha-melanocyte-stimulating hormone (alpha-MSH) and the 150-kD neurofilament protein (NF150): relationship to neurotrophic sequences.
Immunoblot techniques and immunohistochemistry have been used to investigate epitopes shared by alpha-melanocyte-stimulating hormone (alpha-MSH) and the 150-kD neurofilament protein (NF150). Three anti-alpha-MSH antisera (namely 31H2T, 4394, and M5) from a total of 12 sera were found to react with NF150. The three crossreacting sera are different in their binding properties to peptide fragments related to alpha-MSH, suggesting that at least two distinct epitopes are shared by NF150 and alpha-MSH. The only known sequence common to NF150 and alpha-MSH, the N-terminal Ac-S-Y residues, appears to be essential for binding of sera 31H2T and 4394. However, the binding of antiserum M5 involves other, currently unknown, similarities between NF150 and alpha-MSH. This is shown by the binding of M5 to peptides such as ACTH(4-10), which do not contain the N-terminal Ac-S-Y sequence. Binding of M5 to tobacco mosaic virus coat protein (TMV-coat protein), which is homologous with alpha-MSH and NF150 in its Ac-S-Y residues, was negligible. The peptide structures that are recognized by M5 have previously been shown to exert neurotrophic activity. The data are discussed in the light of the hypothesis that similarity between NF150 and alpha-MSH, as illustrated by binding to M5, may be significant in the neurotrophic activity of MSH-related peptides.
D000818:Animals; D036022:Capsid Proteins; D000939:Epitopes / Q000032:analysis*; D005260:Female; D006651:Histocytochemistry; D007158:Immunologic Techniques; D007381:Intermediate Filament Proteins / Q000032:analysis*; D009074:Melanocyte-Stimulating Hormones / Q000032:analysis*; D009417:Nerve Tissue / Q000032:analysis*; D016900:Neurofilament Proteins; D051381:Rats; D011919:Rats, Inbred Strains; D012584:Sciatic Nerve / Q000032:analysis; D013116:Spinal Cord / Q000032:analysis; D014764:Viral Proteins / Q000032:analysis
D036022:Capsid Proteins; D000939:Epitopes; C009496:H protein, Tobacco mosaic virus; D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins; D014764:Viral Proteins; C050934:neurofilament protein 150; D009074:Melanocyte-Stimulating Hormones