Interferon-mediated protection of B16 melanoma cells from cytotoxicity by activated macrophages.
Corynebacterium parvum-activated macrophages (M phi), purified by adherence, were cytotoxic for B16 melanoma cells maintained in vitro. Pretreatment of the melanoma cells for 18 hr with interferon-alpha/beta or -gamma (IFN-alpha/beta or -gamma) caused a reduced susceptibility of the B16 cells to M phi-mediated cytotoxicity. The IFN-induced protective effect of B16 cells from cytotoxic M phi was found to be dose dependent. In addition, IFN-gamma was more protective than IFN-alpha/beta. The protective effect observed with partially purified IFN was reproduced by using highly purified IFN-alpha/beta or recombinant IFN-gamma. Monoclonal antibodies to IFN-gamma neutralized the protective effect provided by IFN-gamma. These results show that the susceptibility of a tumor cell line to killing by activated M phi can be altered by IFN pretreatment.
D000818:Animals; D003602:Cytotoxicity, Immunologic; D007111:Immunity, Cellular; D007372:Interferons / Q000494:pharmacology*; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology*; D008545:Melanoma / Q000276:immunology*; D051379:Mice; D011425:Propionibacterium acnes / Q000276:immunology; D011994:Recombinant Proteins / Q000494:pharmacology
D011994:Recombinant Proteins; D007372:Interferons