Construction of expression vectors for the production of interferons in yeast.
Expression vectors designed for the production of foreign proteins in yeast are constructed as a composite of functional DNA segments brought together in a single plasmid DNA molecule. Such a composite DNA molecule constitutes a complex entity in terms of its replication and selection functions (in both E. coli and S. cerevisiae) and its gene expression properties. The latter depend critically upon transcriptional control signals (the promoter and terminator regions from a highly expressed yeast gene) that are used to direct the expression of foreign genes in yeast cells. This paper describes the construction of such an expression vector based on the transcription control signals of the yeast phosphoglycerate kinase gene and its application to the production of human interferon-alpha's in yeast cells.
D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors; D006801:Humans; D007372:Interferons / Q000235:genetics* / Q000302:isolation & purification; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D011994:Recombinant Proteins; D007372:Interferons