[Detection of tissue-specific gene expression using an in situ hybridization method of histoblotting].
A version of in situ hybridization on the histological sections that is used for screening specific mRNA in tissues in proposed. Sections of the frozen tissue samples are prepared on the cryostatic microtome, placed on nitrocellulose filters and hybridized with labelled DNA-probes under the conditions of RNA blot hybridization. The proposed method ("histoblotting") was used to study the distribution of actin and alpha-phetoprotein mRNA genes in tissues of 15 and 21-day rat embryos. The possibility of studying mRNA (by hibridizations) and protein (by immunoenzyme staining) and making histological analysis simultaneously by histoblotting is demonstrated.
D000818:Animals; D005786:Gene Expression Regulation; D005819:Genetic Markers; D006652:Histological Techniques; D009693:Nucleic Acid Hybridization; D009928:Organ Specificity; D051381:Rats; D011919:Rats, Inbred Strains; D000509:alpha-Fetoproteins / Q000235:genetics
D005819:Genetic Markers; D000509:alpha-Fetoproteins
Vyiavlenie tkanespetsificheskoĭ ékspressii genov metodom gibridizatsii in situ na otpechatkakh gistologicheskikh srezov.