Characterization of trans-acting factor(s) regulating beta-globin gene expression by in vivo competition.
A beta-globin/TK fusion gene was microinjected into non-erythroid cells (Ltk- cells) and erythroid cells (murine erythroleukemia (MEL) cells), and the interactions of the regulatory cellular factors with the beta-globin sequences were investigated by the in vivo competition experiment. The fusion gene was expressed efficiently in Ltk- cells. This expression was inhibited by a co-injection with a three-fold molar excess of the 5'-flanking sequence of the beta-globin gene or with a nine-fold molar excess of the mammary tumor virus LTR, but not with the alpha-globin gene. The fusion gene was expressed very poorly in the uninduced MEL cells and highly in the induced MEL cells. The co-injection of the beta-globin gene did not affect expression in the MEL cells in either uninduced or induced conditions.
D000818:Animals; D001483:Base Sequence; D001609:Beta-Globulins / Q000235:genetics*; D001667:Binding, Competitive; D002454:Cell Differentiation / Q000187:drug effects; D002459:Cell Fusion; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects; D005796:Genes; D005822:Genetic Vectors; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics; D051379:Mice; D008845:Microinjections; D008969:Molecular Sequence Data; D010957:Plasmids; D014157:Transcription Factors / Q000032:analysis / Q000502:physiology*
D001609:Beta-Globulins; D014157:Transcription Factors