Adoptive transfer of murine chronic-relapsing autoimmune encephalomyelitis. Analysis of basic protein-reactive cells in lymphoid organs and nervous system of donor and recipient animals.
Frequency analysis of myelin basic protein (MBP)-reactive lymphocytes was performed in the chronic relapsing murine experimental allergic encephalomyelitis (EAE) model induced by the adoptive transfer of myelin basic protein (MBP)-primed lymphocytes to naive recipients. During the first attack, MBP-reactive cell frequencies were: 1/41,700 in spleen, 1/328,000 in lymph nodes, 1/64,500 in the peripheral blood. After recovery from a second attack, the frequencies were: 1/11,000 in spleen, 1/46,000 in lymph node, and 1/195,000 in the blood. In addition, lymph node cells obtained from animals following a second attack had increased encephalitogenic properties. CNS-derived lymphocytes analyzed during the first attack were 50% Lyt 1.2+ and 16% Lyt 2.2+. After recovery from the second attack, phenotypes were 20% Lyt 1.2+ and 49% Lyt 2.2+. There were only minimal responses to MBP in CNS-derived lymphocytes. Susceptibility to adoptively transferred EAE was in general predicted by whether a proliferative response to MBP occurred following immunization and was not solely H-2 linked. These studies demonstrate an accumulation of autoreactive cells in the spleen and lymph nodes and a shift of the phenotype of cells in the target organ as EAE becomes chronic and suggest there are dynamic immunologic processes, both in the peripheral immune system and target organ associated with relapsing EAE.
D000818:Animals; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology*; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007116:Immunization, Passive; D008198:Lymph Nodes / Q000166:cytology; D051379:Mice; D008815:Mice, Inbred Strains; D004676:Myelin Basic Protein / Q000032:analysis; D009420:Nervous System / Q000166:cytology; D013154:Spleen / Q000166:cytology
D004676:Myelin Basic Protein