title: Effects of ionizing radiation on the metabolism and longitudinal growth of cartilaginous embryonic chick tibiae in vitro.
abstract: The effect of ionizing radiation on the metabolism and longitudinal growth of cartilaginous tibiae of 6.5-day-old chick embryos was studied in vitro over a 3-day period. Before being cultured, tibiae received absorbed doses of 2 to 200 Gy. Of each pair, the counterpart served as control. Compared to the strong inhibition of [3H]thymidine incorporation, already 50 percent at 10 Gy, the effects of ionizing radiation on [3H]uridine and [3H]proline incorporation were limited: 20 and 40 percent respectively at 150 Gy. Metabolism of the cartilage cells in our organ culture was almost completely arrested at 200 Gy. Light and electron microscopy showed no morphological differences between irradiated and sham-irradiated tibiae until 150 Gy. At 200 Gy necrosis of most of the cells was observed. No differences in form and arrangement of extracellular fibers were noticed. The results of the metabolic studies and the morphological observations were correlated with the effects of ionizing radiation on the longitudinal growth. In contrast to DNA synthesis, RNA transcription and synthesis of collagen fibres were radioresistant processes.
mesh_terms: D000818:Animals; D002356:Cartilage / Q000196:embryology / Q000378:metabolism / Q000528:radiation effects*; D002642:Chick Embryo; D003094:Collagen / Q000096:biosynthesis; D004247:DNA / Q000096:biosynthesis; D066298:In Vitro Techniques; D012313:RNA / Q000096:biosynthesis
chemical_list: D012313:RNA; D003094:Collagen; D004247:DNA