Different interferon-producing capacities of L929 cell sublines and the enhancement of interferon production by priming are controlled pretranslationally.
Two sublines of mouse L929 cells designated L929B and L929M were studied. The L929B cells, which displayed a 2-3-fold higher IFN production in response to Sendai virus than that of the L929M cells, had a higher sensitivity to the antiviral and priming effects of IFN and were more resistant to VSV. In good accord with the amount of IFN produced, more translatable IFN mRNA was isolated from the L929B cells. IFN production and IFN mRNA activities were proportionally increased in the IFN-primed cultures of both sublines. Results indicate that both inherent and priming-induced increased-IFN production are based on pretranslational control mechanisms.
D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects; D007372:Interferons / Q000096:biosynthesis* / Q000494:pharmacology; D007739:L Cells; D051379:Mice; D012333:RNA, Messenger / Q000378:metabolism
D012333:RNA, Messenger; D007372:Interferons