Selective inhibition of collagen accumulation by N-(3,4-dimethoxycinnamoyl)anthranilic acid (N-5') in granulation tissue.
The effect of topically applied N-5', an inhibitor of chemical mediator release from mast cells, on the carrageenin-air-pouch inflammation was studied. The formation of granulation tissue, the accumulation of exudate and the number of infiltrating cells were significantly reduced by the treatment with N-5' (100 mg/kg). The collagen content in granulation tissue was dose-dependently reduced without affecting the noncollagen protein and DNA content by treatment with N-5'. At a dose of 100 mg/kg of N-5', prolyl hydroxylase activity in the tissue was significantly decreased. The selective inhibition of collagen accumulation in granulation tissue resulted from reduction of collagen biosynthesis in vivo. N-5' did not directly inhibit collagen synthesis by diploid fibroblasts, but inhibited fibroblast proliferation in culture. Such results indicate that one of the inhibitory mechanisms of collagen accumulation by N-5' in inflamed sites may involve the inhibition of fibroblast proliferation.
D000818:Animals; D002351:Carrageenan; D002455:Cell Division; D003094:Collagen / Q000378:metabolism*; D004247:DNA / Q000032:analysis; D005347:Fibroblasts / Q000378:metabolism; D006097:Granulation Tissue / Q000187:drug effects / Q000378:metabolism*; D007249:Inflammation / Q000139:chemically induced; D008297:Male; D011393:Procollagen-Proline Dioxygenase / Q000378:metabolism; D051381:Rats; D013997:Time Factors; D062367:ortho-Aminobenzoates / Q000494:pharmacology*
D062367:ortho-Aminobenzoates; D002351:Carrageenan; D003094:Collagen; D004247:DNA; D011393:Procollagen-Proline Dioxygenase; C012293:tranilast