title: Inhibition of macromolecular synthesis by caffeine in Clostridium perfringens.
abstract: Caffeine (2 mg/mL) inhibited the incorporation of [14C]adenine into actively growing cells of Clostridium perfringens NCTC 8679 in a dose-dependent manner. Also reduced by caffeine was incorporation of [14C]thymidine and 14C-labeled amino acids. No effect on guanine, uracil, adenosine, guanosine, or uridine was detected. Actual incorporation of [14C]caffeine or [14C]thymine in control cultures did not occur.
mesh_terms: D000225:Adenine / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis*; D002110:Caffeine / Q000494:pharmacology*; D003016:Clostridium perfringens / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004768:Enterotoxins / Q000096:biosynthesis; D012329:RNA, Bacterial / Q000096:biosynthesis*; D013171:Spores, Bacterial; D013936:Thymidine / Q000378:metabolism
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004768:Enterotoxins; D012329:RNA, Bacterial; C035598:enterotoxin, Clostridium; D002110:Caffeine; D000225:Adenine; D013936:Thymidine