Timm-staining intensity is correlated with the density of Timm-positive presynaptic structures in the cerebral cortex of lizards.
In cortical areas of the lizard, Podarcis hispanica, Timm staining reveals a distinct pattern of lamination. At the electron-microscope level, virtually all of the reaction product is located in the synaptic vesicles of Timm-positive boutons. Using linear-regression analysis, the area density of Timm-positive bouton profiles as well as the numerical and volume density of stained vesicles were found to be closely correlated with the light-microscopic densitometric values obtained for each Timm-positive cortical zone. We discuss the possibility of estimating stereological electron-microscopic data parameters from densitometric measurements at the light-microscope level.
D000818:Animals; D002540:Cerebral Cortex / Q000648:ultrastructure*; D003720:Densitometry; D008116:Lizards / Q000033:anatomy & histology*; D008854:Microscopy, Electron; D012044:Regression Analysis; D013194:Staining and Labeling; D013572:Synaptic Vesicles / Q000648:ultrastructure*