[Immunoenzyme method of determination of m5Cyd level; characteristics of the epitope and estimation of m5Cyd levels in the blood of leukemic mice].
Antibodies specific to m5Cyd were obtained and a sensitive enzyme-linked immunosorbent assay (ELISA) for detecting m5Cyd was developed. This method was used for the characterization of m5Cyd epitope. It was shown that the 4-NH2 and 5-CH3-groups of the pyrimidine ring are of primary importance for the antigenic determinant assembly, whereas the ribosyl residue and its structure are less important. It was found also that the amount of m5Cyd in leukemic La murine serum after gamma-irradiation is markedly increased.
D000818:Animals; D003562:Cytidine / Q000031:analogs & derivatives* / Q000097:blood / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D007942:Leukemia, Experimental / Q000097:blood*; D051379:Mice; D008810:Mice, Inbred C57BL
D000939:Epitopes; D003562:Cytidine; C016568:5-methylcytidine
Enzimoimmunologicheskiĭ metod opredeleniia soderzhaniia m5Cyd; kharakteristika épitopa, otsenka soderzhaniia m5Cyd v krovi leĭkoznykh mysheĭ.