Some studies on human pulmonary mast cells obtained by bronchoalveolar lavage and by enzymic dissociation of whole lung tissue.
Human pulmonary mast cells were obtained by bronchoalveolar lavage (BAL) and by enzymic dissociation of whole lung. The cells released histamine on immunological stimulation or on exposure to a hyperosmolar environment. Cell suspensions similarly released newly generated products of arachidonic acid metabolism. Increased numbers of mast cells were recovered by BAL of asthmatic subjects and patients suffering from sarcoidosis and these cells were hyperresponsive to immunological challenge. Mast cells recovered by BAL and enzymic dissociation were differentially inhibited by antiasthmatic drugs. These data emphasize the potential role of BAL mast cells in pulmonary diseases of diverse origin.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D001249:Asthma / Q000473:pathology; D001980:Bronchi / Q000473:pathology*; D002469:Cell Separation / Q000379:methods; D005260:Female; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D006982:Hypertonic Solutions / Q000494:pharmacology; D007073:Immunoglobulin E / Q000276:immunology; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D003012:Microbial Collagenase; D008875:Middle Aged; D015230:Prostaglandin D2; D011457:Prostaglandins D / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000473:pathology*; D013189:SRS-A / Q000378:metabolism; D012507:Sarcoidosis / Q000473:pathology; D007507:Therapeutic Irrigation
D000888:Antibodies, Anti-Idiotypic; D006982:Hypertonic Solutions; D011457:Prostaglandins D; D013189:SRS-A; C026408:anti-IgE antibodies; D007073:Immunoglobulin E; D003012:Microbial Collagenase; D015230:Prostaglandin D2