[Increased selectivity of directed RNA hydrolysis mediated by RNAse H from E. coli using mixed oligo(deoxyribo-ribo)nucleotides].
Chimeric oligo(deoxyribo-ribo)nucleotides appeared to be a valuable tool to achieve the high selectivity of RNA cleavage as shown by RNA-ase H-mediated hydrolysis of TMV RNA directed by d(TGTGTATGCC), d(TGTGTAT), d(TGTGTAT)GCCAU and d(TGTGTAT)ppGCCAU.
D004722:Endoribonucleases; D004926:Escherichia coli / Q000201:enzymology; D006868:Hydrolysis; D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides; D012313:RNA; D016914:Ribonuclease H
D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides; D012313:RNA; D004722:Endoribonucleases; D016914:Ribonuclease H
Povyshenie izbiratel'nosti napravlennogo gidroliza RNK RNKazoĭ H iz Ecoli s pomoshch'iu smeshannykh oligo(dezoksiribo-ribo)-nukleotidov.