Regulation of immune interferon production during the response to soluble microbial antigens.
Human peripheral blood mononuclear cells (PBMC) stimulated in vitro with purified protein derivative (PPD) or with a Candida albicans polysaccharide extract (MPPS) released immune interferon (IFN) and interleukin 2 (IL-2). Kinetic studies showed a biphasic production of IFN with maximum levels at days 3-4 and days 5-6 of culture. In contrast, the IL-2 production is only observed at days 2-3 of culture. The relationship between IFN and IL-2, analysed both in responder and nonresponder PBMC cultures, showed that the early peak of IFN production appears to be IL-2 independent whereas the second peak seems strictly related to the presence of IL-2 culture. Furthermore, monoclonal antibodies against class I and class II products of the major histocompatibility complex (MHC) inhibited IFN production when added at the beginning of culture, whereas only anti-class I antibodies interfered with the release of IFN when added to antigen-primed lymphocytes.
D000328:Adult; D000941:Antigens / Q000276:immunology; D006801:Humans; D007372:Interferons / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000096:biosynthesis*; D007700:Kinetics; D008285:Major Histocompatibility Complex; D008938:Mitosis; D009000:Monocytes / Q000276:immunology*; D011134:Polysaccharides; D014373:Tuberculin
D000941:Antigens; D007376:Interleukin-2; D011134:Polysaccharides; D014373:Tuberculin; D007372:Interferons