The effects of tumor promoters on HTLV-1 expression in a low-virus producer and a non-virus producer cell line.
Chemical agents including 5-iodo-2'-deoxyuridine (IUdR), 4-O-methyl 12-O-tetradecanoyl phorbol-13-acetate (TPA), phorbol, and the tumor promoters teleocidin and TPA were tested for their ability to induce Human T-cell leukemia-lymphoma virus Type I (HTLV-I) expression in the low-virus producer MT-1 cell line and in the non-virus producer C63/CRII-2 cell line, which contains at least one integrated HTLV-I genome equivalent per cell [26]. Viral antigen expression increased from 0.3% to 8.7% in the MT-1 cell line after treatment with IUdR, while TPA and teleocidin were marginally or non-effective as inducers. Chemicals did not induce HTLV-I antigen expression in the C63/CI(II-2) cell line. No enhancement of reverse transcriptase activity or alteration of proviral organization was observed after chemical treatment of MT-1 cells.
D000956:Antigens, Viral / Q000032:analysis; D002273:Carcinogens / Q000494:pharmacology*; D002460:Cell Line; D017977:Deltaretrovirus / Q000187:drug effects* / Q000276:immunology; D007065:Idoxuridine / Q000494:pharmacology; D008235:Lyngbya Toxins / Q000494:pharmacology; D011533:Proviruses / Q000187:drug effects; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000956:Antigens, Viral; D002273:Carcinogens; D008235:Lyngbya Toxins; C022176:teleocidins; D012194:RNA-Directed DNA Polymerase; D007065:Idoxuridine; D013755:Tetradecanoylphorbol Acetate