title: Shared and unique determinants on human and murine intestinal epithelium as established by monoclonal antibodies.
abstract: To characterize macromolecules from intestinal epithelium known to be reactive with human disease sera, monoclonal antibodies (MAbs) were elicited to determine cell localization, organ-specificity and cross-reactivity of the immunodominant determinants. Eight MAbs, reactive with murine epithelial cell-associated components by enzyme-linked immunosorbent assay, immunoblotting and immunofluorescence, demonstrated binding by the same techniques to constituents in determinant-bearing epithelial macromolecules were glycoproteins of approximately 180,000 (human and 199,000 (murine) mol. wt. Pre-incubation of sera from patients with chronic inflammatory bowel disease (but not normal human sera or control protein) inhibited binding of the 199 K macromolecule by three MAbs elicited and characterized in this study.
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D003093:Colitis, Ulcerative / Q000276:immunology*; D003424:Crohn Disease / Q000276:immunology*; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007413:Intestinal Mucosa / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013045:Species Specificity
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes