Detection of the intracellular ras p21 oncogene product by flow cytometry.
Rapid identification of the expression of oncogene products in specific cell types could potentially be useful in the diagnosis and treatment of human malignancy. We have now observed that through the use of lysolecithin permeabilization and fluorescence-activated flow cytometry, cells expressing high levels of the v-Ha-ras oncogene product, p21, can readily be distinguished from the nontransformed parent cells in a rapid and quantitative manner.
D000818:Animals; D000911:Antibodies, Monoclonal; D002463:Cell Membrane Permeability; D002472:Cell Transformation, Viral; D000939:Epitopes; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006251:Harvey murine sarcoma virus; D008244:Lysophosphatidylcholines; D051379:Mice; D008807:Mice, Inbred BALB C; D009857:Oncogenes; D011518:Proto-Oncogene Proteins / Q000032:analysis*; D016283:Proto-Oncogene Proteins p21(ras); D051381:Rats
D000911:Antibodies, Monoclonal; D000939:Epitopes; D008244:Lysophosphatidylcholines; D011518:Proto-Oncogene Proteins; D016283:Proto-Oncogene Proteins p21(ras)