title: Potential drugs for elimination of acute lymphatic leukemia cells from autologous bone marrow.
abstract: Five drugs, selected because of minimal in vivo myelotoxicity, have been investigated for inhibition of the growth of three acute lymphatic leukemia-derived cell lines. Granulocyte-macrophage colony-forming units (GM-CFU) inhibition with these five drugs after 60-min incubation was first established. Drug concentrations giving up to 90% kill of GM-CFU were then used. Spirogermanium and L-asparaginase did not have an effect on any of the three cell lines under the culture conditions tested, while 4-hydroperoxycyclophosphamide (4-HC) and vincristine inhibited the growth of all three cell lines tested, and bleomycin inhibited the growth of two cell lines. In addition to 4-HC, bleomycin and vincristine should be considered as possible agents in eliminating leukemic cells from autologous marrow grafts. These drugs also deserve further investigation in clonal systems.
mesh_terms: D000970:Antineoplastic Agents / Q000633:toxicity*; D001215:Asparaginase / Q000633:toxicity; D001761:Bleomycin / Q000633:toxicity; D001853:Bone Marrow / Q000187:drug effects / Q000473:pathology; D001854:Bone Marrow Cells; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003520:Cyclophosphamide / Q000031:analogs & derivatives / Q000633:toxicity; D005857:Germanium / Q000633:toxicity; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D007945:Leukemia, Lymphoid / Q000473:pathology*; D009942:Organometallic Compounds; D013141:Spiro Compounds / Q000633:toxicity; D014750:Vincristine / Q000633:toxicity
chemical_list: D000970:Antineoplastic Agents; D009942:Organometallic Compounds; D013141:Spiro Compounds; D005857:Germanium; D001761:Bleomycin; C026818:spirogermanium; D014750:Vincristine; D003520:Cyclophosphamide; D001215:Asparaginase; C011272:perfosfamide