Scanning electron microscopy of rabbit tubes at different intervals after anastomosis using fibrin glue.
Eighteen New Zealand White rabbits were submitted to tubal anastomosis using fibrin glue. The animals were then killed at different intervals (2-56 days) and the tubes were removed for analysis with the scanning electron microscope in order to study re-epithelialization and ciliogenesis. For the first 4 days after the operation the epithelium seemed to suffer considerable traumatic effects and was de-epithelialized. On the 6th day cells with microvilli appeared and towards the 10th day the first cilia were seen. Ciliogenesis was complete between 2 and 4 weeks after the operation, at which time the cilia re-acquired the ability to beat in coordination, as well as attaining normal quantity and length. The time required for recovery by the epithelium after anastomosis with fibrin glue would thus seem similar to that required when other suture materials are used. Fibrin glue thus combines the advantage of a considerable reduction in operating times with the ability to be rapidly reabsorbed, with complete restitutio ad integrum of the tissues.
D000714:Anastomosis, Surgical; D000818:Animals; D007611:Aprotinin / Q000627:therapeutic use*; D002923:Cilia / Q000648:ultrastructure; D004338:Drug Combinations / Q000627:therapeutic use; D004848:Epithelium / Q000648:ultrastructure; D005069:Evaluation Studies as Topic; D005176:Factor XIII / Q000627:therapeutic use*; D005187:Fallopian Tubes / Q000601:surgery / Q000648:ultrastructure*; D005260:Female; D015718:Fibrin Tissue Adhesive; D005340:Fibrinogen / Q000627:therapeutic use*; D008855:Microscopy, Electron, Scanning; D008866:Microsurgery; D011817:Rabbits; D011897:Random Allocation; D013917:Thrombin / Q000627:therapeutic use*; D013997:Time Factors
D004338:Drug Combinations; D015718:Fibrin Tissue Adhesive; D005340:Fibrinogen; D005176:Factor XIII; D007611:Aprotinin; D013917:Thrombin