The effect of nucleotides on glutamate dehydrogenase from the mealworm fat body.
The effect of nucleotides: AMP, cAMP, ADP, ATP, GDP and GTP, on glutamate dehydrogenase (GDH) purified from the mealworm fat body was studied. Guanine nucleotides and ATP inhibited the enzyme strongly in both directions. GDH was partially protected from the inhibition by the addition of ADP to an assay medium. AMP and cAMP activated the enzyme slightly. The concerted effects of ADP and ATP indicate the importance of adenylate energy charge in the regulation of fat body GDH. It is suggested that GDH may play amphibolic role in the fat body and that the direction of GDH catalysed reaction is under strong influence of nucleotides. The enzyme may synthesize glutamate at high energy charge, but when the energy reserves are low, it oxidizes glutamate.
D000273:Adipose Tissue / Q000201:enzymology*; D000818:Animals; D005216:Fat Body / Q000201:enzymology*; D005969:Glutamate Dehydrogenase / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism; D009711:Nucleotides / Q000502:physiology*; D013712:Tenebrio / Q000201:enzymology*
D005971:Glutamates; D009711:Nucleotides; D005969:Glutamate Dehydrogenase