A staining method to distinguish hemopoietic cells from stromal cells in long-term marrow culture.
A method is described that permits easy morphological recognition of hemopoietic and stromal cells in the adherent layer of long-term marrow culture in light microscopy. The method relies on the solubilization of plasma membrane with Triton X-100 to permit differential staining of cytoskeleton in the two cell types.
D000818:Animals; D001854:Bone Marrow Cells; D002462:Cell Membrane / Q000187:drug effects; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D017830:Octoxynol; D009924:Organ Culture Techniques / Q000379:methods; D011092:Polyethylene Glycols / Q000494:pharmacology; D013194:Staining and Labeling / Q000379:methods*
D011092:Polyethylene Glycols; D017830:Octoxynol