Na+ transport by reconstituted Na+,K+-ATPase in the presence of various nucleotides.
The ability of ATP, CTP, ITP, GTP, UTP and two synthetic ATP analogs to provide for ouabain-sensitive Na+ accumulation into proteoliposomes with a reconstituted Na+,K+-ATPase (ATP phosphohydrolase, EC 3.6.1.37) was investigated. A correlation between the proton-accepting properties of the nucleotides and their ability to provide for active transport was found. The proton-accepting properties of the substrate seem to be a necessary condition for the shift from the K-form of Na+,K+-ATPase--an immutable step in the active translocation of Na+ and K+ through the Na+ pump.
D000818:Animals; D001693:Biological Transport, Active; D066298:In Vitro Techniques; D007473:Ion Channels / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D011510:Proteolipids; D011522:Protons; D012265:Ribonucleotides / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013329:Structure-Activity Relationship
D007473:Ion Channels; D011510:Proteolipids; D011522:Protons; D012265:Ribonucleotides; C020485:proteoliposomes; D012964:Sodium; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium