[Preparation of an anti-idiotypic antibody directed against anti-dexamethasone antibody and demonstration of its ability to acknowledge the receptor of glucocorticoids].
By using a 3-carboxymethyloxime dexamethasone derivative coupled to bovine serum albumin we have prepared specific anti-dexamethasone antibodies in rabbits. These antibodies were then purified by affinity chromatography and administered to a second set of rabbits. One of them produced anti-idiotypic antibodies able to impede the [3H] dexamethasone binding of the initial anti-dexamethasone antibodies and to displace the [3H] dexamethasone-antibodies complexes towards high molecular weight species in gel filtration experiments. Moreover these antibodies we were also able to impede the binding of [3H] dexamethasone to the rat liver glucocorticoid receptor and to recognize the highly purified receptor using Western blot analysis.
D000818:Animals; D003907:Dexamethasone / Q000276:immunology*; D000939:Epitopes; D007130:Immunoglobulin Idiotypes / Q000096:biosynthesis* / Q000276:immunology; D011817:Rabbits; D011965:Receptors, Glucocorticoid / Q000276:immunology*
D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D011965:Receptors, Glucocorticoid; D003907:Dexamethasone
Préparation d'un anticorps anti-idiotypique dirigé contre un anticorps anti-dexaméthasone et mise en évidence de sa capacité à reconnaître le récepteur des glucocorticoïdes.