Expression of pp60v-src alters the ionic permeability of the plasma membrane in rat cells.
The transmembrane potential of Rous sarcoma virus (RSV)-infected Rat-1 cells, expressing the pp60v-src protein kinase, is markedly less negative (by approximately 30 mV) than that of their normal counterparts. By contrast, the membrane potential of Rat-1 cells infected with Kirsten sarcoma virus is virtually unaltered. The RSV-induced membrane depolarization is shown to be due to a severalfold increase in the cation permeability ratio (PNa/PK) of the plasma membrane. When cells infected with a temperature-sensitive mutant of RSV (ts LA29), encoding a src protein with heat-labile kinase activity, are shifted from the nonpermissive to the permissive temperature, a rapid and sustained membrane depolarization is observed. Conversely, thermal inactivation of the ts LA29 pp60v-src kinase activity rapidly restores the membrane potential to near normal levels. Addition of epidermal growth factor, platelet-derived growth factor, or insulin to uninfected cells fails to cause a detectable change in membrane potential. We conclude that, unlike growth factor receptor tyrosine kinases, pp60v-src can induce, either directly or indirectly, a major change in the membrane permeability to monovalent cations.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects; D002472:Cell Transformation, Viral; D004815:Epidermal Growth Factor / Q000494:pharmacology; D006358:Hot Temperature; D007328:Insulin / Q000494:pharmacology; D007708:Kirsten murine sarcoma virus / Q000378:metabolism; D008433:Mathematics; D008564:Membrane Potentials / Q000187:drug effects; D015688:Oncogene Protein pp60(v-src); D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011494:Protein Kinases / Q000096:biosynthesis; D011518:Proto-Oncogene Proteins / Q000494:pharmacology; D016392:Proto-Oncogene Proteins pp60(c-src); D051381:Rats; D012191:Retroviridae Proteins / Q000096:biosynthesis* / Q000494:pharmacology; D013696:Temperature
D007328:Insulin; D010982:Platelet-Derived Growth Factor; D011518:Proto-Oncogene Proteins; D012191:Retroviridae Proteins; D004815:Epidermal Growth Factor; D011494:Protein Kinases; D015688:Oncogene Protein pp60(v-src); D016392:Proto-Oncogene Proteins pp60(c-src)