Probable identity of NILE glycoprotein and the high-molecular-weight component of L1 antigen.
We report here that anti-L1 antiserum, raised against material from embryonic brain, and anti-NILE antiserum, raised against purified NILE (nerve growth factor-inducible large external) glycoprotein of PC12 cells, immunoprecipitate from PC12 cells material of the same apparent molecular weight (230 kilodaltons) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Furthermore, each of these immune reagents has the capacity to clear from a PC12 cell extract all of the 230-kilodalton antigen recognized by the other antiserum. Finally, in immunohistochemical staining of developing cerebellum the two antisera exhibit very similar staining patterns. We suggest that the NILE glycoprotein and the high-molecular-weight component of L1 antigen are closely related molecules, and probably the same.
D000310:Adrenal Gland Neoplasms; D000818:Animals; D000954:Antigens, Surface / Q000276:immunology*; D002460:Cell Line; D002531:Cerebellum / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology*; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology*; D006651:Histocytochemistry; D007106:Immune Sera / Q000276:immunology*; D007124:Immunoenzyme Techniques; D007163:Immunosorbent Techniques; D039841:Leukocyte L1 Antigen Complex; D051379:Mice; D008970:Molecular Weight; D009414:Nerve Growth Factors; D039842:Neural Cell Adhesion Molecule L1; D010673:Pheochromocytoma
D000954:Antigens, Surface; D000939:Epitopes; D006023:Glycoproteins; D007106:Immune Sera; D039841:Leukocyte L1 Antigen Complex; D009414:Nerve Growth Factors; D039842:Neural Cell Adhesion Molecule L1