title: Cytochemical characteristics of the axon membrane at nodes of Ranvier in resting, tetrodotoxin-blocked, and electrically stimulated peripheral nerves of the rat.
abstract: To test whether intraaxonal ferric ion-ferrocyanide staining at nodes of Ranvier is influenced by functional state of the nodal membrane, the tibial nerves of Sprague-Dawley rats were subjected to one of the following experimental procedures prior to fixation and staining: General anesthesia to induce nerves at rest, tetrodotoxin blocking of nerve activity, and high-frequency (100 Hz) electrical stimulation. In all cases most but not all nodes were stained. No statistically significant differences were found either in the percentage of total stained nodes or in the percentage of stained nodes from large- and small-diameter fibers among the three conditions tested. An explanation is offered to account for the apparent discrepancy between these results and those from other studies involving related cytochemical markers of the nodal apparatus.
mesh_terms: D000768:Anesthesia, General; D000818:Animals; D001369:Axons / Q000032:analysis*; D002462:Cell Membrane / Q000032:analysis; D004558:Electric Stimulation; D005290:Ferric Compounds; D005295:Ferrocyanides; D010525:Peripheral Nerves / Q000032:analysis* / Q000187:drug effects; D011901:Ranvier's Nodes / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling; D013779:Tetrodotoxin / Q000494:pharmacology; D014048:Tolonium Chloride
chemical_list: D005290:Ferric Compounds; D005295:Ferrocyanides; D014048:Tolonium Chloride; D013779:Tetrodotoxin