Efficient replication of HTLV-III and STLV-IIImac in human Jurkat cells.
High producer cell lines were established by infecting Jurkat cells with either HTLV-IIIB from H9 cells or with STLV-IIImac from HUT-78 cells. The Jurkat cells produced both viruses at least 10 times more efficiently then the original cell lines; the pelleted virus from Jurkat cells was also less contaminated with non-virion proteins. Accordingly, a higher specificity was attained in an ELISA for antibodies to HTLV-III if the antigenic activity was derived from the virus from Jurkat cells, as opposed to that from H9 cells.
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology; D000818:Animals; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000276:immunology; D002460:Cell Line; D006678:HIV / Q000254:growth & development*; D006801:Humans; D007945:Leukemia, Lymphoid; D008253:Macaca mulatta / Q000382:microbiology; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D013601:T-Lymphocytes / Q000382:microbiology*; D014764:Viral Proteins / Q000032:analysis; D014779:Virus Replication
D000956:Antigens, Viral; D014764:Viral Proteins; D012194:RNA-Directed DNA Polymerase