Cryoprecipitation. In vitro studies on fibronectin as an independent cryoprecipitagogue.
Fifteen patients affected by essential mixed cryoglobulinemia or secondary cryoglobulins were studied. Fibronectin was detected in the majority of essential mixed cryoglobulinemias, but in a minority of secondary cryoglobulins. In vitro studies gave strong evidence of an interaction between fibronectin and heat-aggregated gammaglobulins. The effects of fibronectin on cryoprecipitation of heat-aggregated gammaglobulins seem to support the independent role of the glycoprotein in the cryoformation mechanism, although its presence does not appear essential for cryoglobulin appearance.
D011232:Chemical Precipitation; D003080:Cold Temperature; D003166:Complement Activating Enzymes / Q000378:metabolism; D015922:Complement C1q; D003449:Cryoglobulinemia / Q000097:blood; D003450:Cryoglobulins / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D005353:Fibronectins / Q000302:isolation & purification*; D006358:Hot Temperature; D006801:Humans; D007122:Immunoelectrophoresis; D011092:Polyethylene Glycols / Q000494:pharmacology; D011485:Protein Binding; D012217:Rheumatoid Factor / Q000378:metabolism; D005719:gamma-Globulins / Q000378:metabolism
D003450:Cryoglobulins; D005353:Fibronectins; D005719:gamma-Globulins; D011092:Polyethylene Glycols; D015922:Complement C1q; D012217:Rheumatoid Factor; D003166:Complement Activating Enzymes