Secondary structure of a herpes simplex virus glycoprotein D antigenic domain.
The peptide 6-amino caproyl-Pro-Ser-Leu-Lys-Met-Ala-Asp-Pro-Asn-Arg-Phe-Arg-Gly-Lys-Asp-Leu- Pro-6- amino caproate has been synthesized and its secondary structure has been investigated by 1H n.m.r. at 400 MHz. Resonances were assigned from 2D NOESY and COSY spectra, and the secondary structure was determined using NOEs, three-bond coupling constants, and exchange rates of amide protons. The peptide has two tight turns centered on the Pro-Asn and Arg-Gly pairs. The relationship between the secondary structure found here and the antigenic nature of the peptide is discussed.
D000595:Amino Acid Sequence; D000939:Epitopes / Q000032:analysis*; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D010446:Peptide Fragments / Q000138:chemical synthesis; D018139:Simplexvirus / Q000032:analysis*; D014759:Viral Envelope Proteins / Q000138:chemical synthesis*
D000939:Epitopes; D007202:Indicators and Reagents; D010446:Peptide Fragments; D014759:Viral Envelope Proteins; C039447:glycoprotein D, Human herpesvirus 1