title: The effects of some polysulphated polysaccharides on hyaluronate (HA) synthesis by human synovial fibroblasts.
abstract: The in vitro synthesis of HA in synovial fibroblasts derived from normal and rheumatoid joints was investigated. Incubation of these cells with ArteparonR and a variety of sulphated dextrans showed that macromolecular HA synthesis could be modified by these drugs. ArteparonR and a dextran sulphate (DS1) of molecular weight 20,600 and 0.8 sulphate substitution stimulated HA synthesis in rheumatoid cells by approximately 150%. In contrast the dextrans with higher sulphate substitution but similar molecular weight were less effective. Gel filtration chromatography of media from drug-treated cells showed an increase in HA hydrodynamic size.
mesh_terms: D001172:Arthritis, Rheumatoid / Q000378:metabolism; D016264:Dextran Sulfate; D003911:Dextrans / Q000494:pharmacology*; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology*; D066298:In Vitro Techniques; D008970:Molecular Weight; D013329:Structure-Activity Relationship; D013583:Synovial Membrane / Q000378:metabolism*
chemical_list: D003911:Dextrans; D006820:Hyaluronic Acid; D016264:Dextran Sulfate