A method suitable for the isolation of monoclonal antibodies from large volumes of serum-containing hybridoma cell culture supernatants.
A method suitable for the isolation of monoclonal antibodies from large volumes of serum-containing hybridoma cell culture supernatants is described. The purification is carried out in three steps; buffer exchange by chromatography on Sephadex G-25, fractionation by cation exchange chromatography on S Sepharose Fast Flow and, as a final step, gel filtration on Sephacryl S-200 HR. Experience from purification of 11 different mouse monoclonal antibodies is reported.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D002845:Chromatography / Q000379:methods; D003470:Culture Media / Q000032:analysis; D003911:Dextrans; D006825:Hybridomas / Q000032:analysis*; D051379:Mice; D012685:Sepharose
D000911:Antibodies, Monoclonal; D003470:Culture Media; D003911:Dextrans; D012685:Sepharose; C025614:sephadex