Cell mediated PPD specific cytotoxicity against human monocyte targets: II. IL-2 expansion improves the strength and discriminatory power of CTLs used for cellular typing.
Large batches of Cytotoxic T Lymphocytes (CTLs) specific for antigenic components of Purified Protein Derivative of tuberculin (PPD) were generated from 20 donors. Peripheral Blood Mononuclear cells (PBM) were stimulated with PPD, expanded with Interleukin-2 (IL-2) and finally aliquotted and cryopreserved. It was found that CTLs could be cryopreserved without loss of activity, and compared to generating CTLs in primary culture alone, IL-2 expanded CTLs were stronger and discriminated better. To study their HLA restriction specificity, the CTLs were tested against a panel of 50 target cell donors using PPD pulsed monocytes as antigen presenting target cells. Reproducibility was examined by analysis of variance. Guided by the results, the mean value of 2 tests was selected as the basis for qualitative assignments.
D000704:Analysis of Variance; D000954:Antigens, Surface / Q000032:analysis; D002478:Cells, Cultured; D003201:Computers; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D000939:Epitopes / Q000032:analysis; D005615:Freezing; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D009000:Monocytes / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology*; D014373:Tuberculin / Q000494:pharmacology*
D000954:Antigens, Surface; D000939:Epitopes; D006680:HLA Antigens; D007376:Interleukin-2; D014373:Tuberculin