Epitope localization of a monoclonal antibody, LO-22, with broad specificity for interferon-alpha subtypes.
A murine monoclonal antibody, LO-22, with broad cross-reactivity to human interferon-alpha (HuIFN-alpha) subtypes and some animal IFN-alpha species was found to bind less efficiently to IFN-alpha A (IFN-alpha 2a). In contrast, LO-22 bound strongly to IFN-alpha 2 (IFN-alpha 2b) and IFN-alpha 2C (IFN-alpha 2c) which differ by one or two amino acids, respectively, from IFN-alpha A; the latter has lysine at position 23 whereas the other closely related IFNs have arginine. LO-22 also bound efficiently to IFN-alpha D which is only 83% related to IFN-alpha A, but which also has arginine at position 23. These results strongly suggest that LO-22 recognizes a conserved epitope among IFN-alpha subtypes in which arginine at position 23 is involved. The specificity of a second monoclonal antibody, MT4/E4, is also reported and compared to that of LO-22.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001120:Arginine / Q000276:immunology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes / Q000032:analysis*; D006801:Humans; D007370:Interferon Type I / Q000276:immunology*; D051379:Mice
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007370:Interferon Type I; D001120:Arginine