Contact insensitivity of a subpopulation of normal human fetal kidney epithelial cells and of human carcinoma cell lines.
Early passage normal human fetal kidney epithelial cells were inoculated on top of a confluent monolayer of X-ray lethally irradiated human fibroblasts to determine the colony-forming ability of these epithelial cells. The results indicate that the great majority of the epithelial cells did not have the clonogenic ability on the fibroblast cell mat, although they were capable of colony formation on plastic surface without the cell mat. A small subpopulation of these epithelial cells, however, was able to proliferate on the cell mat. These contact-insensitive fetal epithelial cells were found to be deficient in gap junction-mediated intercellular communication, to contain keratin and gamma-glutamyl transpeptidase but not fibronectin. These contact-insensitive cells appear to have greater proliferative potential than the parental cell population and to exist transiently in early passage but not in late passage culture. The ability of proliferation on cell mat was found to be shared by 22 different human carcinoma cell lines that were tested. This unique clonogenic ability of normal contact-insensitive and human carcinoma cells on the cell mat could provide a selection method for presumptive normal stem and tumor cells and for an assay for screening potential antitumor drugs and assessing the efficacy of chemotherapeutic drugs against a given tumor.
D002277:Carcinoma / Q000473:pathology*; D002450:Cell Communication; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D004847:Epithelial Cells; D005333:Fetus / Q000166:cytology; D006801:Humans; D007633:Keratins / Q000096:biosynthesis; D007668:Kidney / Q000166:cytology*
D007633:Keratins