Development and characterization of monoclonal antibodies to bovine brain calcineurin.
Three monoclonal antibodies (MAb's) against bovine brain calcineurin, a Ca++-calmodulin dependent phosphatase, have been developed and characterized. Among these antibodies, two are alpha-subunit (60,000 Mr) specific and one is beta-subunit (19,000 Mr) specific. These antibodies also cross-react with similar proteins obtained from brains of human, rat and mouse. The methodology for raising these antibodies and their properties are described.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000936:Antigen-Antibody Complex; D001921:Brain / Q000201:enzymology*; D019703:Calcineurin; D002148:Calmodulin-Binding Proteins / Q000032:analysis / Q000037:antagonists & inhibitors / Q000276:immunology*; D002417:Cattle; D002460:Cell Line; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis; D006825:Hybridomas / Q000276:immunology; D007700:Kinetics; D046911:Macromolecular Substances; D051379:Mice; D008970:Molecular Weight; D010749:Phosphoprotein Phosphatases / Q000032:analysis / Q000037:antagonists & inhibitors / Q000276:immunology*; D010954:Plasmacytoma; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D002148:Calmodulin-Binding Proteins; D000939:Epitopes; D046911:Macromolecular Substances; D019703:Calcineurin; D010749:Phosphoprotein Phosphatases