Further resolution of human alpha-fetoprotein by affinity electrophoresis with erythroagglutinating phytohemagglutinin of Phaseolus vulgaris lectin.
Major molecular species of human alpha-fetoprotein(AFP), which were separated as single components by serial affinity chromatography with concanavalin A(Con-A) and Lens culinaris agglutinin, were further resolved into several bands by affinity electrophoresis with erythroagglutinating phytohemagglutinin of Phaseolus vulgaris lectin(E-PHA). Among the newly separated main molecular species, both Con-A- and E-PHA-reactive AFP(AFP-1X1) was demonstrated, contrary to the known sugar specificity of Con-A and E-PHA, in addition to molecular species of AFP reacting with Con-A but not with E-PHA(AFP-1X0) and of AFP reacting with E-PHA but not with Con-A(AFP-0X1). AFP-0X1 was formed from AFP-0X0, and AFP-1X1 from AFP-1X0 by neuraminidase treatment; thus, AFP-0X1 and AFP-1X1 represent asialylated and AFP-0X0 and AFP-1X0 sialylated molecular species. AFP-1X1' and AFP-0X0' were present as minor components. AFP-0X0' had no affinity for E-PHA, and the affinity increased in the order of AFP's-0X0(or 0X1), -1X1', -1X1 and -0X1. Proportions of those components varied depending on the pathophysiological conditions of AFP production.
D001212:Asialoglycoproteins; D006528:Carcinoma, Hepatocellular / Q000032:analysis; D002846:Chromatography, Affinity / Q000379:methods; D003208:Concanavalin A; D004586:Electrophoresis / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D060748:Fetuins; D006801:Humans; D008113:Liver Neoplasms / Q000032:analysis; D008649:Mesonephroma / Q000032:analysis; D009369:Neoplasms / Q000032:analysis*; D009373:Neoplasms, Germ Cell and Embryonal / Q000032:analysis; D010835:Phytohemagglutinins / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000032:analysis; D013379:Substrate Specificity; D000509:alpha-Fetoproteins / Q000032:analysis / Q000302:isolation & purification*
D001212:Asialoglycoproteins; D060748:Fetuins; D010835:Phytohemagglutinins; D000509:alpha-Fetoproteins; C017029:asialofetuin; D003208:Concanavalin A