Evaluation of the presence of RF-like activities in immunoglobulin preparations for intravenous use. I. IgG anti-IgE in intravenous Ig.
We have studied the IgG anti-IgE antibody content of 8 IgG preparations for intravenous administration that we use in our Day Hospital as replacement therapy for patients with hypogammaglobulinemia. In all preparations we found the presence of both IgE and IgG anti-IgE as monomers and oligomers after fractionation with ultracentrifugation on sucrose gradient. This RF-like activity seem to be an essential constituent of low m.w. aggregates, disappearing at acid pH. We suggest that its presence could be responsible for the enrichment of IgE in Ig preparations for therapeutic use and perhaps for immediate and long-term adverse reactions.
D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D001323:Autoantibodies / Q000032:analysis*; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis / Q000276:immunology*; D007074:Immunoglobulin G / Q000032:analysis*; D005719:gamma-Globulins / Q000592:standards
D000888:Antibodies, Anti-Idiotypic; D001323:Autoantibodies; D007074:Immunoglobulin G; C026408:anti-IgE antibodies; D005719:gamma-Globulins; D007073:Immunoglobulin E