Distribution of tightly bound non-histone proteins in chromatin fractions obtained by DNAase II digestion.
Digestion of pig liver chromatin with DNAse II afforded three different fractions which were characterized in terms of their DNA, RNA and tightly bound non-histone protein content, their DNA fragment size and their template activity. Two of these fractions are soluble after digestion with DNAase II and have been separated on the basis of their different solubility in MgCl2. A third fraction is not solubilized even after extensive digestion, although the size of its DNA is comparable to that of the enzyme solubilized fractions. The three fractions show qualitatively and quantitatively different distribution of tightly bound non-histone proteins, with specific protein components in each fraction; furthermore the non-solubilized fraction is greatly enriched in proteins tightly bound to DNA. From all the data obtained it can be suggested that the tightly bound proteins of the insoluble fraction may play, directly or indirectly, a role in maintaining an organized chromatin structure.
D000818:Animals; D002843:Chromatin / Q000032:analysis* / Q000302:isolation & purification; D002868:Chromosomal Proteins, Non-Histone / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D004706:Endodeoxyribonucleases / Q000378:metabolism / Q000494:pharmacology*; D008099:Liver / Q000166:cytology; D008274:Magnesium / Q000494:pharmacology; D015636:Magnesium Chloride; D010316:Particle Size; D011485:Protein Binding; D012313:RNA / Q000032:analysis; D013552:Swine; D013997:Time Factors
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D015636:Magnesium Chloride; D012313:RNA; D004706:Endodeoxyribonucleases; C019329:deoxyribonuclease II; D008274:Magnesium