Measurement of total HDL, HDL2 and HDL3 by dextran sulfate-MgCl2 precipitation technique in human serum.
We describe a simple and reliable method for determination of total HDL, HDL2, and HDL3 by a precipitation technique using dextran-sulfate (Mr 50,000)-Mg2+. A combined solution of dextran sulfate and Mg2+ at their respective final concentration of 0.9 g/l and 27 mmol/l was optimal for separating total HDL from the other lipoproteins. The present method compares favorably with a heparin-MnCl method (r = 0.998). The HDL was further resolved into HDL2 and HDL3 by addition of a combined solution of dextran sulfate and Mg2+ (1.5 m/l and 10 g/l) to the total HDL solution. Comparison of this precipitation method with the well-established ultracentrifugation method yielded the mean correlation coefficient of 0.941 and 0.869 for HDL2 and HDL3, respectively.
D011232:Chemical Precipitation; D008076:Cholesterol, HDL / Q000097:blood; D016264:Dextran Sulfate; D003911:Dextrans; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood* / Q000302:isolation & purification; D053439:Lipoproteins, HDL2; D053440:Lipoproteins, HDL3; D008274:Magnesium; D015636:Magnesium Chloride
D008076:Cholesterol, HDL; D003911:Dextrans; D008075:Lipoproteins, HDL; D053439:Lipoproteins, HDL2; D053440:Lipoproteins, HDL3; D015636:Magnesium Chloride; D016264:Dextran Sulfate; D008274:Magnesium