Gastrin induction of protein synthesis in isolated gastric mucosal cells.
Exposure of isolated rat gastric mucosal cells to 10(-10) and 10(-9) M gastrin (G-17-I) for 2 hr significantly stimulated [3H]leucine incorporation (15 min pulse) into protein by 100 and 212%, respectively, when compared with the basal levels. Doses beyond 10(-9) M lowered the maximal stimulatory effect of the hormone. Gastrin (10(-9) M) specifically stimulated the synthesis of five proteins in isolated gastric mucosal cells with apparent molecular weights of 105, 76, 71, 63, and 54 kDa. Actinomycin-D (10 micrograms/ml) completely abolished the gastrin-mediated stimulation of protein synthesis in isolated gastric mucosal cells.
D000818:Animals; D003609:Dactinomycin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D005753:Gastric Mucosa / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005755:Gastrins / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D008970:Molecular Weight; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains
D005755:Gastrins; D003609:Dactinomycin; D012313:RNA