Effects of substance P and substance K on the growth of cultured keratinocytes.
The effects of substance P and substance K, which are coexpressed in the same mRNA as a beta-preprotachykinin in peripheral tissues and released in the inflammatory lesion of the skin, were examined on epidermal proliferation using spontaneously transformed mouse epidermal cell line (Pam 212 cells). Substance P stimulated the synthesis of DNA of Pam 212 cells in the medium containing 2%-10% fetal calf serum (FCS). Stimulation of DNA synthesis was dose dependent if the cells were cultured in the medium containing 2% FCS (quiescent condition). This effect was inhibited by spantide. In a serum-free medium, substance P had no effect on keratinocyte proliferation. In contrast, substance K, which shares a common amino acid sequence with substance P on its C-terminal, did not affect DNA synthesis of Pam 212 cells in either medium condition. Substance P released in inflammation may stimulate epidermal proliferation.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D004817:Epidermis / Q000187:drug effects*; D007633:Keratins; D051379:Mice; D008807:Mice, Inbred BALB C; D015288:Neurokinin A; D009479:Neuropeptides / Q000494:pharmacology*; D013373:Substance P / Q000031:analogs & derivatives / Q000494:pharmacology*
D009479:Neuropeptides; D013373:Substance P; D007633:Keratins; D015288:Neurokinin A; D004247:DNA; C042867:spantide