Definition of a differentiation antigen on the surface of phagocytic cells of thymic reticulum which is down-regulated by interferon gamma.
Monoclonal antibodies (MoAb) were raised against phagocytic cells of thymic reticulum (P-TR) grown in vitro. Each of the two MoAb (TR-1N, TR-3N) defined two polypeptides of 46-57 kDa on P-TR membrane. TR-1N and TR-3N recognize respectively 48 and 81% of P-TR, but do not recognize any cells in spleen, lymph node, thymic lymphocytes, or bone marrow. They bind to part of peritoneal macrophages and to macrophage cell lines J 774 and P 388 D1. Cell binding of TR-1N and TR-3N was compared by immunofluorescence to that of anti-CR3 antibody (Mac-1) which recognizes P-TR, a small number of cells in bone marrow and spleen, and a much higher percentage of peritoneal macrophages. The polypeptides recognized by TR-1N/TR-3N may be defined as differentiation antigens on accessory cells as they appear on bone marrow cells during maturation in vitro in the presence of L-cell supernatant which contains colony stimulating factor (CSF-1). Interferon gamma is able to down-regulate the expression of TR-1N/TR-3N antigen on P-TR membrane while that of Mac-1 is unchanged and that of Ia is up-regulated.
D000818:Animals; D000911:Antibodies, Monoclonal; D000938:Antigen-Presenting Cells / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000032:analysis* / Q000276:immunology; D001854:Bone Marrow Cells; D000939:Epitopes / Q000032:analysis; D005260:Female; D005455:Fluorescent Antibody Technique; D007371:Interferon-gamma / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains; D010586:Phagocytes / Q000032:analysis* / Q000187:drug effects; D011961:Receptors, Fc / Q000187:drug effects; D012159:Reticulum / Q000166:cytology* / Q000187:drug effects; D012397:Rosette Formation; D013950:Thymus Gland / Q000166:cytology* / Q000187:drug effects
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D000939:Epitopes; D011961:Receptors, Fc; D007371:Interferon-gamma