Isolation of cDNA clones corresponding to the Mr = 150,000 subunit of chick type VI collagen.
Type VI collagen is a disulfide-bonded protein with an unusual structure in that the molecule contains three short triple-helical domains and very extended non-collagenous regions. The molecule is a heterotrimer composed in the chick of two polypeptides of similar apparent size in SDS-PAGE (Mr = 140- and 150,000) but different structure, and a third component that is much larger (Mr = 260,000) than the other two chains. We report here on the isolation of several overlapping cDNA clones from a chicken aorta mRNA expression library in the plasmid vector pEX1. Antibodies affinity purified onto the fusion proteins recognized the chick type VI collagen Mr = 150,000 subunit. Northern blots using the cDNA inserts from the above clones revealed a single RNA species of about 4,600 nucleotides sufficient to code for a protein with the size of the Mr = 150,000 subunit.
D000818:Animals; D002645:Chickens; D003094:Collagen / Q000235:genetics* / Q000276:immunology; D004247:DNA / Q000302:isolation & purification*; D008970:Molecular Weight; D012313:RNA / Q000032:analysis
D012313:RNA; D003094:Collagen; D004247:DNA