[Rapid diagnosis of experimental tumor by FITC-Con A lectin--a comparative study of smear and section specimens].
FITC-Con A staining as a rapid diagnostic method for tumor cells was applied to the tumors smeared on glass slide and section specimens to evaluate the reactivity with FITC-Con A. Good staining results were obtained in smear specimens with clear fluorescence on the membrane of tumor cells. Con A and LCH lectins bound well with tumor cells to produce strong fluorescence in comparison with PEA and DBA. It indicates that tumor cells expressed dominantly the receptors of alpha-D-glucose and alpha-D-mannose sugar chain on the membrane of tumor cells. From these results it was concluded that FITC-Con A staining method applied to smear specimens is more advantageous in the rapidity and the simplicity for tumor cell diagnosis than section specimen method.
D000818:Animals; D003110:Colonic Neoplasms / Q000473:pathology*; D003208:Concanavalin A / Q000031:analogs & derivatives*; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives*; D005452:Fluoresceins; D051379:Mice; D008807:Mice, Inbred BALB C; D008867:Microtomy; D013048:Specimen Handling / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*; D014407:Tumor Cells, Cultured
D005452:Fluoresceins; C042784:fluorescein isothiocyanate-concanavalin A; D003208:Concanavalin A; D016650:Fluorescein-5-isothiocyanate