Identification of biologically active natural spirolactone derivatives in man and animal.
For the first time, the presence of three natural spirolactones hydroxylated at C6C7 (6 alpha, 7 alpha-, 6 beta, 7 alpha- and 6 beta, 7 beta-dihydroxy-6,7-dihydrocanrenone (DHC) is demonstrated in man and in animal urine (rat, dog, sheep), and possibly in the blood. The existence of the fourth isomer 6 alpha, 7 beta- is also possible. Salt-loading in man and the rat results in a decrease in urinary 6 alpha, 7 alpha- and 6 beta, 7 beta-DHC. Salt-depletion increases their urinary concentration in the rat. DHC isomers are not found in the urine of adrenalectomized rats. Injected into the caudal vein of the rat, both 6 alpha, 7 alpha- and 6 beta, 7 beta-DHC induce a significant retention of Na+. On the other hand, 6 beta, 7 alpha-DHC significantly increases Na+ and K+ excretion. The biological activities of these natural compounds seem to be different from those of synthetic spirolactonic drugs.
D000315:Adrenalectomy; D000328:Adult; D000368:Aged; D000818:Animals; D002192:Canrenone / Q000031:analogs & derivatives / Q000302:isolation & purification* / Q000494:pharmacology; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005260:Female; D006801:Humans; D007536:Isomerism; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D009318:Natriuresis; D011188:Potassium / Q000652:urine; D011245:Pregnadienes / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000494:pharmacology; D013237:Stereoisomerism
D011245:Pregnadienes; D002192:Canrenone; D012964:Sodium; D011188:Potassium