A substance P antagonist also inhibits specific binding and mitogenic effects of vasopressin and bombesin-related peptides in Swiss 3T3 cells.
While vasopressin and peptides of the bombesin family bind to different receptors in quiescent Swiss 3T3 cells, the antagonist [D-Arg1,D-pro2,D-Trp7,9,Leu11] substance P blocks the specific binding of both (3H) vasopressin and 125I-gastrin-releasing peptide to these cells. In addition, the antagonist inhibits the mobilization of Ca2+ and induction of DNA synthesis by vasopressin. These results indicate that [D-Arg1,D-Pro2,D-Trp7,9,Leu11] substance P has the ability to interact with the receptors for three structurally unrelated peptide hormones.
D000818:Animals; D001127:Arginine Vasopressin / Q000037:antagonists & inhibitors*; D001839:Bombesin / Q000037:antagonists & inhibitors*; D002118:Calcium / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D051379:Mice; D011945:Receptors, Angiotensin / Q000187:drug effects; D018004:Receptors, Bombesin; D011956:Receptors, Cell Surface / Q000187:drug effects; D011949:Receptors, Cholecystokinin; D017483:Receptors, Vasopressin; D013373:Substance P / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000494:pharmacology
D011945:Receptors, Angiotensin; D018004:Receptors, Bombesin; D011956:Receptors, Cell Surface; D011949:Receptors, Cholecystokinin; D017483:Receptors, Vasopressin; D001127:Arginine Vasopressin; D013373:Substance P; C038073:substance P, Arg(1)-Pro(2)-Trp(7),(9)-LeuNH(2)(11)-; D004247:DNA; D001839:Bombesin; D002118:Calcium