Ion channels activated by acetylcholine and gamma-aminobutyric acid in freshly dissociated sympathetic neurones of the rat.
Transmitter-activated channels in freshly isolated neurones from sympathetic ganglia of young rats have been examined using the patch-clamp technique. Acetylcholine (ACh), gamma-aminobutyric acid (GABA) and (in about one third of cells) glycine produced an inward current and an increase in current noise when perfused onto voltage-clamped neurones. The ACh noise was fitted with a single Lorentzian spectrum with a time constant of 13.1 ms at -70 mV. Outside-out membrane patches allowed high-resolution measurements of single ACh- and GABA-activated channels. The ACh-channels had a conductance of 30 pS.
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D005260:Female; D005728:Ganglia, Sympathetic / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D007473:Ion Channels / Q000145:classification / Q000187:drug effects* / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D011930:Reaction Time / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
D007473:Ion Channels; D005680:gamma-Aminobutyric Acid; D000109:Acetylcholine