A membrane protein in LRM55 glial cells cross-reacts with antibody to the anion exchange carrier of human erythrocytes.
Cl-/HCO3-exchange transport in the glial plasma membrane is remarkably similar to anion exchange transport in erythrocytes. When LRM55 cells were pretreated with the extracellular labeling reagent [125I]iodosulfanilic acid, radioactivity appeared primarily in two bands on denaturing polyacrylamide gels at Mr 70,000 and 30,000. Antibody to the human erythrocyte anion exchanger (Band 3) cross-reacted on immunoblots with an LRM55 band at Mr 68,000. The LRM55 Cl-/HCO3-exchanger may be a component of Mr 68,000 to 70,000.
D001457:Anion Exchange Protein 1, Erythrocyte / Q000276:immunology*; D027321:Anion Transport Proteins; D001798:Blood Proteins / Q000276:immunology*; D002352:Carrier Proteins / Q000276:immunology*; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology*; D004910:Erythrocyte Membrane / Q000276:immunology*; D006801:Humans; D008565:Membrane Proteins / Q000276:immunology*; D009457:Neuroglia / Q000276:immunology*
D001457:Anion Exchange Protein 1, Erythrocyte; D027321:Anion Transport Proteins; D001798:Blood Proteins; D002352:Carrier Proteins; D000939:Epitopes; D008565:Membrane Proteins