title: Heat-shock protein synthesis by human polymorphonuclear cells.
abstract: Mature human neutrophils from peripheral blood are known to be capable of limited protein biosynthetic activity. We now show that these cells are inducible for heat-shock protein synthesis when placed in short-term culture. Synthesis of hsp70 and hsp85 as well as the response to various temperatures and the time course of induction were typical for mammalian cell systems. This heat-shock response was blocked by actinomycin D added before heat exposure. This demonstration that hsp genes can be activated by heat exposure of terminally differentiated neutrophils supports the hypothesis that gene activation can serve a physiological role in these cells and opens up the possibility that synthesis of other gene products is similarly inducible.
mesh_terms: D003609:Dactinomycin / Q000494:pharmacology; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D009504:Neutrophils / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis; D013997:Time Factors; D014158:Transcription, Genetic
chemical_list: D006360:Heat-Shock Proteins; D003609:Dactinomycin; D012313:RNA