Monoclonal antiidiotypic antibodies which recognized the binding site of delta receptor: fine specificity of the antiidiotypic antibodies.
Monoclonal antiidiotypic antibodies (Mab2), specific for the binding unit of the opiate delta receptor, were generated in rats immunized with rabbit anti-DADLE antibodies (ab 1). The fine specificity of the seven clones screened in the fusion was studied using a solid phase test where the ab 1 antibodies were coated to the plate. In this respect, 4 different set of monoclonal antibodies can be distinguished. The antibody affinities were comparable (Kd 1.10(-8)M), but the specificity patterns were completely different.
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex / Q000032:analysis; D004743:Enkephalin, Leucine / Q000031:analogs & derivatives / Q000378:metabolism; D016308:Enkephalin, Leucine-2-Alanine; D000939:Epitopes / Q000032:analysis; D007130:Immunoglobulin Idiotypes; D007700:Kinetics; D051381:Rats; D011957:Receptors, Opioid / Q000276:immunology* / Q000378:metabolism; D017465:Receptors, Opioid, delta
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D011957:Receptors, Opioid; D017465:Receptors, Opioid, delta; D004743:Enkephalin, Leucine; D016308:Enkephalin, Leucine-2-Alanine