Excitation-secretion coupling: ionic currents in glomerulosa cells: effects of adrenocorticotropin and K+ channel blockers.
The ionic conductance of cultured rat glomerulosa cells has been studied using the whole cell variant of the patch-clamp technique. We have identified and partially characterized three currents: a transient outward current, a slow outward current, and a slow inward current. The transient outward current activated rapidly and then inactivated slowly on maintained depolarization. Activation was initiated at -30 mV, and zero current was seen at -60 to -50 mV. The slow outward current did not inactivate with time and was initiated around 0 mV; its zero current voltage was difficult to evaluate. The two outward currents were present in different proportions, which explains the different time course of the total outward current from one cell to another. A slow inward current was also found which activated near -30 mV and reached its reversal potential between 80 and 100 mV. This current was blocked by Co2+, increased with [Ca2+]o, and was insensitive to Na+-free external medium. ACTH, a potent stimulant of steroid output, was found to block the transient outward current, but was ineffective on the slow outward current and the slow inward current. Tetraethylammonium and 4-aminopyridine, K+ channel inhibitors, also blocked the transient outward current.
D015761:4-Aminopyridine; D000311:Adrenal Glands / Q000187:drug effects / Q000502:physiology*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000631:Aminopyridines / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002412:Cations; D003035:Cobalt / Q000494:pharmacology; D004553:Electric Conductivity; D007473:Ion Channels / Q000187:drug effects* / Q000502:physiology; D011188:Potassium / Q000378:metabolism*; D051381:Rats; D019789:Tetraethylammonium; D013757:Tetraethylammonium Compounds / Q000494:pharmacology
D000631:Aminopyridines; D002412:Cations; D007473:Ion Channels; D013757:Tetraethylammonium Compounds; D003035:Cobalt; D019789:Tetraethylammonium; D000324:Adrenocorticotropic Hormone; D015761:4-Aminopyridine; D011188:Potassium; D002118:Calcium