[Effect of endogenous fibrinolysis activation on human plasminogen].
Plasminogen preparation from donor blood and fibrinolytically active blood plasma from humans after sudden death were obtained using affinity chromatography on Lysin-sepharose 4B. The plasminogen preparation from donor blood was shown to be highly purified native plasminogen (Glu-plasminogen). The preparation containing activated plasminogen (Lys-plasminogen), plasmin, plasminogen activator, alpha 2-macroglobulin, alpha 1-antitrypsin, fibrin/fibrinogen was obtained from the blood plasma of humans after sudden death. The appearance of proteins lacking biological specificity to lysin-sepharose in the plasminogen preparation shows the ability of activated plasminogen and plasmin to form complexes with these proteins and demonstrates the retention of the functional activity in lysin-binding regions on their molecules. Monospecific sera to the isolated preparations were obtained, demonstrating the presence of the same immunochemical determinants in native and activated plasminogen.
D002846:Chromatography, Affinity; D003645:Death, Sudden; D004789:Enzyme Activation; D005341:Fibrinolysin / Q000032:analysis; D005342:Fibrinolysis; D006801:Humans; D005779:Immunodiffusion; D010958:Plasminogen / Q000032:analysis / Q000502:physiology*; D010960:Plasminogen Activators / Q000097:blood; D000515:alpha 1-Antitrypsin / Q000032:analysis; D000511:alpha-Macroglobulins / Q000032:analysis
D000515:alpha 1-Antitrypsin; D000511:alpha-Macroglobulins; D010958:Plasminogen; D010960:Plasminogen Activators; D005341:Fibrinolysin
Vliianie éndogennoi aktivatsii fibrinoliza na plazminogen cheloveka.