Isobutyl cyanoacrylate nanoparticles as a solid phase for an efficient immunoradiometric assay.
The interaction of monoclonal antibodies and seric proteins with cyanoacrylic nanoparticles was investigated. A high binding capacity, rapid kinetics of adsorption as well as a good stability of the linkage were found. Furthermore, the immunoreactivity was conserved after coating antibody to nanoparticles. A new competitive immunoradiometric assay was also proposed for determination of alpha-fetoprotein amounts as low as 0.1 ng per assay.
D000818:Animals; D000911:Antibodies, Monoclonal; D001672:Biocompatible Materials; D001798:Blood Proteins; D002015:Bucrylate; D003487:Cyanoacrylates; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D051379:Mice; D008863:Microspheres; D010316:Particle Size; D011137:Polystyrenes; D011863:Radioimmunoassay / Q000379:methods*; D000509:alpha-Fetoproteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D001672:Biocompatible Materials; D001798:Blood Proteins; D003487:Cyanoacrylates; D007140:Immunoglobulin Fab Fragments; D011137:Polystyrenes; D000509:alpha-Fetoproteins; D002015:Bucrylate