title: A correlation between changes in conformation and molecular properties of bovine serum albumin upon succinylation.
abstract: Using succinic anhydride, six succinylated derivatives of bovine serum albumin having percent modification in the range of 23-87% were prepared and their physicochemical and immunological properties were studied. Measurements of Stokes radius, frictional ratio, UV spectra, solvent perturbation, solubility, and immunological cross-reactivity against anti-bovine serum albumin antiserum revealed that the protein undergoes gradual changes in its native conformation with increase in the degree of succinylation. These changes were less marked below 50% modification but became pronounced above 50% modification. However, even the maximally modified preparation (87%) contained a significant amount of folded structure. Interestingly, though the measurements of various molecular properties revealed significant changes in 23-49% modified preparations, the solubility parameters for these preparations which were obtained at high ionic strength were indistinguishable from those of the native protein. The various results taken together suggest that at lower degrees of chemical modification, the conformational changes were produced mainly because of an increase in electrostatic free energy, whereas at higher degrees of modification, steric hindrance in addition to the electrostatic factor seems to make a substantial contribution to the conformational changes in the modified proteins.
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D002627:Chemistry, Physical; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D011487:Protein Conformation; D012710:Serum Albumin, Bovine / Q000276:immunology; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet; D013386:Succinates; D013387:Succinic Anhydrides
chemical_list: D000939:Epitopes; D007106:Immune Sera; D013386:Succinates; D013387:Succinic Anhydrides; D012710:Serum Albumin, Bovine; C031801:succinic anhydride