[Use of monoclonal antibodies in comparative studies of Ca, Mg-dependent endonuclease in cell nuclei].
Eight hybridoma cell lines derived from fusion between myeloma X-63 and mouse splenocytes were found to secrete monoclonal antibodies against Ca/Mg-dependent endonuclease of human spleen cell nuclei. Two of them, termed N and S, were used in comparative research of enzymes from different organs and species of animals. The data obtained show that N and S antibodies recognize different antigenic determinants of the enzyme molecule. Cross-reactions of antibodies with different antigens having similar antigenic determinants, exist in Ca/Mg-endonuclease of such species as man, mouse, rat and cattle. The evolutionary conservatism of this enzyme is suggested. The data show that the existence of tissue-specific (thymus-specific and spleen-specific) isoforms of Ca/Mg-endonuclease of cell nuclei is possible.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002417:Cattle; D002467:Cell Nucleus / Q000201:enzymology*; D004706:Endodeoxyribonucleases / Q000276:immunology* / Q000378:metabolism; D000939:Epitopes / Q000276:immunology; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology; D051379:Mice; D051381:Rats; D013045:Species Specificity; D013154:Spleen / Q000201:enzymology; D013950:Thymus Gland / Q000201:enzymology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D004706:Endodeoxyribonucleases; C029367:calcium magnesium dependent endodeoxyribonuclease
Primenenie monoklonal'nykh antitel v sravnitel'nykh issledovaniiakh Ca, Mg-zavisimoĭ éndonukleazy kletochnykh iader.