Neuron responses to substance P and enkephalin in rat dorso-lateral septum in vitro.
Using an in vitro brain slice technique the responses of spontaneously active neurons in the rat dorso-lateral septum to 10 nM substance P (SP) and enkephalin were determined. Fewer neurons responded to SP (41%) than to enkephalin (55%). The SP responses were 13 excitations, 14 inhibitions, the enkephalin responses were 13 excitations, 14 inhibitions and 11 responded to both, 6 of these were inhibited by both. Immunocytochemical techniques have shown there is a discrete localisation of SP and enkephalin axons and terminals in the rat septum. SP responsive neurons were associated with the SP terminal-rich region (p = 0.01) but no association was found for enkephalin responses in the enkephalin terminal-rich region (p = 0.7).
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D004744:Enkephalin, Methionine / Q000031:analogs & derivatives* / Q000494:pharmacology; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012688:Septum Pellucidum / Q000187:drug effects / Q000502:physiology*; D013373:Substance P / Q000494:pharmacology*
D013373:Substance P; D004744:Enkephalin, Methionine; C022118:enkephalinamide-Met, Ala(2)-