Presence of fucolipid antigens with mono- and dimeric X determinant (Lex) in the circulating immune complexes of patients with adenocarcinoma.
A series of fucosylated glycosphingolipids with the Lewisx (Lex) determinant (Gal beta 1----4[Fuc alpha 1----3]GlcNAc) have been shown to accumulate in human adenocarcinomas. Lex glycolipids were eluted from Protein A-silica columns over which plasma from patients with adenocarcinoma had previously been perfused. The fact that Protein A has strong affinity for IgG and IgG-immune complexes suggested that the Lex antigens isolated from Protein A eluates were complexed with IgG. Lewisx antigen eluted from Protein A columns banded in the immune complex-enriched region (below IgG) of neutral sucrose density gradients. A modified Raji cell assay and an anticomplement C1q enzyme-linked immunosorbent assay were also used for measurement of Lex antigen associated with C3- and C1q-CIC, respectively. Following affinity purification of Lex-IgG complexes and subsequent dissociation of these immune complexes, human antibodies were isolated which reacted with purified glycosphingolipids containing Lex. Levels of Lex-IgG complexes were found to be 2- to 5-fold higher in eluates of Protein A-silica columns perfused with plasma from adenocarcinoma patients compared to eluates from columns perfused with plasma from healthy individuals and patients with other cancers. These assays may prove to be of diagnostic and/or prognostic significance in adenocarcinoma.
D000230:Adenocarcinoma / Q000276:immunology*; D000906:Antibodies / Q000032:analysis; D000936:Antigen-Antibody Complex / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000032:analysis*; D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity; D003166:Complement Activating Enzymes / Q000276:immunology; D003172:Complement C1 / Q000276:immunology; D015922:Complement C1q; D000939:Epitopes / Q000032:analysis*; D006017:Glycolipids / Q000276:immunology*; D006801:Humans
D000906:Antibodies; D000936:Antigen-Antibody Complex; D000951:Antigens, Neoplasm; D003172:Complement C1; D000939:Epitopes; D006017:Glycolipids; C025567:fucolipids; D015922:Complement C1q; D003166:Complement Activating Enzymes