title: Serum IgG antibody to outer membrane antigens of Pseudomonas cepacia and Pseudomonas aeruginosa in cystic fibrosis.
abstract: IgG antibody to the outer membrane of Pseudomonas cepacia was quantified in sera from controls without cystic fibrosis (CF) and from Pseudomonas aeruginosa- and P. cepacia-infected and noninfected patients with CF. The mean antibody titer in the P. cepacia-infected group was significantly higher than that in the other three groups; the mean titer in the P. aeruginosa-infected group was significantly higher than that in the noninfected and control groups. Preabsorption of CF sera with P. cepacia outer membrane produced a significantly lower mean antibody concentration than did matched samples preabsorbed with an equal amount of lipopolysaccharide. By western blot, significantly more P. cepacia-infected patients produced IgG to the 27- than the 36-kilodalton (kDa) porin protein of P. cepacia; 12 of 16 P. aeruginosa-infected patients produced IgG to the 27-kDa porin. Sera from all patients in both groups contained IgG to the porin protein of P. aeruginosa by western blot. We conclude that the 27-kDa porin of P. cepacia is antigenic in most P. cepacia-infected patients with CF and that some P. cepacia outer membrane components may be antigenically related to those of P. aeruginosa.
mesh_terms: D000293:Adolescent; D000328:Adult; D000942:Antigens, Bacterial / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D002648:Child; D003550:Cystic Fibrosis / Q000150:complications* / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007118:Immunoassay; D007074:Immunoglobulin G / Q000032:analysis*; D007473:Ion Channels; D008297:Male; D018272:Porins; D011549:Pseudomonas / Q000276:immunology; D011552:Pseudomonas Infections / Q000150:complications / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001425:Bacterial Outer Membrane Proteins; D007074:Immunoglobulin G; D007473:Ion Channels; D018272:Porins