Studies of a human lambda-chain epitope related to a complementarity-determining region.
A tryptic non-adecapeptide representing the 24-42 sequence of the MCG lambda-type Bence-Jones protein, and which contains its entire complementarity-determining region-1, was isolated. The peptide was utilized in preparing an affinity column that was used to isolate an antibody having the reactivity of a previously employed idiotypic antibody to MCG. This antibody preparation, as well as 13 monoclonal mouse antibodies to human lambda-chains, was employed in an enzyme-linked immunoassay to detect other Bence-Jones proteins with this serologic specificity. The results obtained with two of the monoclonal antibodies suggest that the epitope in question is a noncontiguous one.
D000596:Amino Acids / Q000032:analysis; D000915:Antibody Affinity; D001536:Bence Jones Protein / Q000276:immunology; D001667:Binding, Competitive; D002846:Chromatography, Affinity; D000939:Epitopes / Q000032:analysis*; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000276:immunology*; D007146:Immunoglobulin lambda-Chains / Q000276:immunology*
D000596:Amino Acids; D000939:Epitopes; D007135:Immunoglobulin Variable Region; D007146:Immunoglobulin lambda-Chains; D001536:Bence Jones Protein