title: The Drosophila ninaC locus encodes two photoreceptor cell specific proteins with domains homologous to protein kinases and the myosin heavy chain head.
abstract: The fruit fly Drosophila melanogaster has been extensively used to identify genes required for photoreceptor cell function. We show that the ninaC gene, originally isolated as a Drosophila visual mutation with an electrophysiological phenotype, encodes two novel cytoskeletal proteins. We identified the DNA sequences encoding the ninaC gene by rescuing the electrophysiological phenotype using P-element-mediated germ line transformation. The ninaC locus is expressed as two extensively overlapping mRNAs encoding proteins of 1135 and 1501 amino acids. Both proteins contain a putative protein kinase domain joined to a domain homologous to the head region of the myosin heavy chain and are spatially restricted to photoreceptor cells.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004274:DNA, Recombinant; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development; D004591:Electrophoresis, Polyacrylamide Gel; D005091:Exons; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D007118:Immunoassay; D007438:Introns; D008969:Molecular Sequence Data; D009154:Mutation; D009218:Myosins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010786:Photoreceptor Cells / Q000378:metabolism*; D011494:Protein Kinases / Q000235:genetics*; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012689:Sequence Homology, Nucleic Acid; D014170:Transformation, Genetic
chemical_list: D004274:DNA, Recombinant; D007106:Immune Sera; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D012313:RNA; D004247:DNA; D011494:Protein Kinases; D009218:Myosins