title: The small nuclear ribonucleoprotein SS-B/La binds RNA with a conserved protease-resistant domain of 28 kilodaltons.
abstract: SS-B/La is a nuclear protein of 48 kilodaltons with two structural domains of Mr 28,000 and Mr 23,000 generated by proteolytic cleavage. UV irradiation was used to cross-link preexisting intracellular La-RNA complexes. Subsequent protease digestion and diagonal gel electrophoresis showed that the RNA-binding site resided in the nonphosphorylated, methionine-rich 28-kilodalton domain.
mesh_terms: D001324:Autoantigens / Q000302:isolation & purification* / Q000528:radiation effects; D001665:Binding Sites; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008970:Molecular Weight; D011487:Protein Conformation; D012313:RNA / Q000378:metabolism* / Q000528:radiation effects; D012261:Ribonucleoproteins / Q000276:immunology / Q000378:metabolism* / Q000528:radiation effects; D017411:Ribonucleoproteins, Small Nuclear; D014466:Ultraviolet Rays; D000097585:SS-B Antigen
chemical_list: D001324:Autoantigens; D012313:RNA; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear; D000097585:SS-B Antigen