Glucocorticoid receptors are associated with particles containing DNA and RNA in vivo.
Chemical crosslinking of glucocorticoid-receptor complexes to associated components in living cells was performed by the use of formaldehyde. Glucocorticoid binding sites were predominantly located in nuclei, and could not be efficiently extracted by 0.3 M NaCl. Sonication was found to cause the release of about 40% of nuclear receptor complexes. By sucrose density gradient centrifugation of soluble extracts from nuclear sonicates, crosslinked receptor complexes were found in oligomeric forms under high salt conditions. Treatment of these extracts with hydrolytic enzymes showed that DNA and RNA were associated with crosslinked receptor complexes.
D002451:Cell Compartmentation; D002467:Cell Nucleus / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D005557:Formaldehyde; D006367:HeLa Cells; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D009698:Nucleoproteins / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*; D011965:Receptors, Glucocorticoid / Q000378:metabolism*
D009687:Nuclear Proteins; D009698:Nucleoproteins; D011965:Receptors, Glucocorticoid; D005557:Formaldehyde; D012313:RNA; D004247:DNA; D007770:L-Lactate Dehydrogenase