title: Preservation of resuspended red cell concentrate. Analysis of purines, nucleosides and nucleotides in stored red cells.
abstract: Purine nucleotides of red blood cells (RBC) during storage in two different media with addition of adenine/nicotinamide (NAP) or adenine/guanosine (CDS-AG) were estimated by HPLC. Synthesis of guanine nucleotides reached a maximum after 14 days in RBC stored with adenine/guanosine. The higher adenine concentration in the NAP solution (3 mmol/l) did not increase adenine consumption and the ATP-level of the erythrocytes. The adenylate energy charge (AEC) of RBC decreased from 0.91 to 0.63 during 42 days of storage in CDS-AG solution.
mesh_terms: D000227:Adenine Nucleotides / Q000097:blood*; D001793:Blood Preservation / Q000379:methods*; D017707:Erythrocyte Transfusion; D004912:Erythrocytes / Q000378:metabolism; D006150:Guanine Nucleotides / Q000097:blood*; D006801:Humans
chemical_list: D000227:Adenine Nucleotides; D006150:Guanine Nucleotides