Fluorescence-activated cell sorter (FACS) analysis of rabbit cells.
Monoclonal antibodies RB1, RB2, RT1, RT2 and RB3 were prepared against rabbit lymphoid cells by immunization with various fractions of rabbit lymphoid cells. The antigens detected by the antibodies are found on B and T cells in different densities. High proportions of polymorphonuclear and bone marrow cells which do not carry the RABELA and RTLA antigens carry the antigens of the RB and RT series. A subpopulation of appendix sIg-negative, RTLA-negative cells has a relatively high concentration of RT2. In general, B and T cells of the appendix show relatively small differences in the membrane densities of RB and RT antigens.
D000818:Animals; D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000032:analysis; D001402:B-Lymphocytes / Q000145:classification*; D002469:Cell Separation; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D006649:Histocompatibility Antigens / Q000032:analysis; D008297:Male; D011817:Rabbits / Q000276:immunology*; D013601:T-Lymphocytes / Q000145:classification*
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D000939:Epitopes; D006649:Histocompatibility Antigens