title: Nucleotide sequence of a tRNA(leu)CAG gene from Rhizobium meliloti.
abstract: The nucleotide sequence of a tRNA(leu)CAG gene from Rhizobium meliloti has been determined. Its organization includes a short transcript with a promoter-like structure and two Rho-independent terminators about 10 bp downstream from the cloverleaf structure. The putative promoter shows good homology with the -45 and -35 region of the consensus (sigma 70) promoter sequence of Escherichia coli, but less with the -10 region. A discriminator-like sequence is present between the start of the transcript and the -10 region. The gene shows extensive homology to tRNA(leu)CAG genes from Gram-positive and Gram-negative bacteria. However, significant differences are encountered in the variable loop region. This is the first report of a tRNA sequence from R. meliloti.
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics*; D012231:Rhizobium / Q000235:genetics*
chemical_list: D012329:RNA, Bacterial; D012343:RNA, Transfer