Synthesis of fusion proteins containing antigenic determinants of foot-and-mouth disease virus.
Part of the genome of foot-and-mouth disease virus (FMDV) type 01,BFS, including the sequence encoding the capsid polypeptide VP1, was cloned in Escherichia coli following a new cloning strategy. The clone containing the VP1 sequence was used for the construction of two expression plasmids encoding VP1 fusion proteins. Subsequently, substantial amounts of the two VP1-beta-galactosidase fusion proteins, containing either one (amino acid region 140-160) or two (amino acid regions 140-160 and 200-213) antigenic determinants of the virus, were synthesized by E. coli bacteria. The protein containing the amino acid region 140-160 of VP1 fused to beta-galactosidase efficiently induced antibodies in rabbits specifically reacting with FMDV type 01,BFS. The same protein was also capable of eliciting neutralizing antibodies. The fusion protein containing both antigenic determinants did not efficiently induce antibodies reacting with FMDV.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral / Q000276:immunology*; D005537:Aphthovirus / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D004274:DNA, Recombinant / Q000378:metabolism; D000939:Epitopes / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics; D007114:Immunization; D010957:Plasmids; D012367:RNA, Viral / Q000302:isolation & purification; D011817:Rabbits; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*; D014760:Viral Fusion Proteins; D001616:beta-Galactosidase
D000914:Antibodies, Viral; D000956:Antigens, Viral; D004274:DNA, Recombinant; D000939:Epitopes; D012367:RNA, Viral; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; D004247:DNA; D001616:beta-Galactosidase