title: [The histochemical use of alcian green].
abstract: With the hydrochloric acid-alcoholic solution of alcian green, without any pretreatment both the sulfated and the carboxylated proteoglycans can be demonstrated. After an indirect sulfation (periodic acid-bisulfite pretreatment), the glycogen and the glycoproteins, and after an oxidation with acidified potassium permanganate, the high cystine-containing proteins can also be stained. As a counterstain, a picric acid-Sirius red solution has proved good.
mesh_terms: D004396:Coloring Agents; D006003:Glycogen / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis; D011509:Proteoglycans / Q000032:analysis; D013194:Staining and Labeling; D045725:Tetrapyrroles
chemical_list: D004396:Coloring Agents; D006023:Glycoproteins; D011509:Proteoglycans; D045725:Tetrapyrroles; C044886:alcian green; D006003:Glycogen
vernacular_title: Uber die histochemische Anwendung des Alciangrüns.