Specific radioimmunoprecipitation of histone H2A antigens by protein A conjugated sepharose.
A modified radioimmunoprecipitation technique is described which allows the specific detection of histone H2A antigens. The technique circumvents unspecific binding of histones to the bacterial adsorbent.
D000818:Animals; D000941:Antigens / Q000032:analysis / Q000276:immunology; D004175:Diptera / Q000378:metabolism; D004331:Drosophila melanogaster / Q000032:analysis; D006657:Histones / Q000032:analysis* / Q000276:immunology; D007163:Immunosorbent Techniques; D014176:Protein Biosynthesis; D012313:RNA / Q000378:metabolism; D013205:Staphylococcal Protein A; D013462:Sulfur Radioisotopes
D000941:Antigens; D006657:Histones; D013205:Staphylococcal Protein A; D013462:Sulfur Radioisotopes; D012313:RNA