Electrophoresis of honey: characterization of trace proteins from a complex biological matrix by silver staining.
The proteins of unconcentrated honey have been detected with the methylamine-incorporating silver stain (T. Marshall, 1984, Anal. Biochem. 136, 340-346) following sodium dodecyl sulfate-polyacrylamide gel electrophoresis or high-resolution two-dimensional electrophoresis. The former consistently reveals at least 19 protein bands in a variety of Australian honeys. Two-dimensional electrophoresis gave patterns of poor resolution but proved useful for further characterization of the major protein constituents. The protein patterns were unaffected by centrifugation of the samples prior to preparation for electrophoresis.
D004591:Electrophoresis, Polyacrylamide Gel; D006722:Honey / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D012834:Silver; D012967:Sodium Dodecyl Sulfate; D013194:Staining and Labeling
D011506:Proteins; D012967:Sodium Dodecyl Sulfate; D012834:Silver