Different localization of receptors for omega-conotoxin and nitrendipine in rat brain.
The bindings of radioiodinated omega-conotoxin GVIA and [3H]-nitrendipine to subcellular fractions of rat brain were examined. The results indicated that omega-conotoxin binding site was mainly present in the mitochondrial fraction, whereas nitrendipine binding site was rich in the mitochondrial but also present in the post-mitochondrial fraction. Fractionation of the mitochondrial fraction on a sucrose density gradient centrifugation showed that the both binding sites were localized in the heavy synaptosomal fraction. These results strongly suggest that the N- and L-type voltage-sensitive calcium channels have different localizations.
D000818:Animals; D001923:Brain Chemistry; D015220:Calcium Channels; D007473:Ion Channels / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D008978:Mollusk Venoms / Q000378:metabolism*; D009568:Nitrendipine / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D011978:Receptors, Nicotinic / Q000378:metabolism*; D013574:Synaptosomes / Q000378:metabolism; D020866:omega-Conotoxin GVIA; D020960:omega-Conotoxins
D015220:Calcium Channels; D007473:Ion Channels; D008978:Mollusk Venoms; D017981:Receptors, Neurotransmitter; D011978:Receptors, Nicotinic; D020960:omega-Conotoxins; C053157:omega-conotoxin receptor; C053423:Conus magus toxin; D020866:omega-Conotoxin GVIA; D009568:Nitrendipine