title: Transcellular transport of fluorescein dextran through an arterial endothelial cell monolayer.
abstract: Transcellular transport of fluorescein dextran (FD) of various molecular weights (4K, 10K, 20K, 70K and 150K daltons) through porcine arterial endothelial cells cultured on a type I collagen gel supported by a dacron sheet was studied and compared with the transport of low density lipoprotein labeled with rhodamine B (RB-LDL) described previously (Hashida et al., Cell Struct. Funct. 11, 31-42, 1986). The rate of FD transport through the monolayer depended on the size of the FD. FD transport was not temperature-dependent and was not a saturable process. Our findings show that FD transport differs from RB-LDL transport which is temperature- and dose-dependent. The mechanism of the transport of FD is compared with that of RB-LDL.
mesh_terms: D000818:Animals; D001158:Arteries / Q000166:cytology*; D001693:Biological Transport, Active; D002478:Cells, Cultured; D003911:Dextrans / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004727:Endothelium / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives*; D005452:Fluoresceins / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000378:metabolism; D008970:Molecular Weight; D012235:Rhodamines / Q000378:metabolism; D013552:Swine; D013696:Temperature
chemical_list: D003911:Dextrans; D005452:Fluoresceins; D008077:Lipoproteins, LDL; D012235:Rhodamines; C015219:fluorescein isothiocyanate dextran; D016650:Fluorescein-5-isothiocyanate; C029773:rhodamine B