Binding characteristics and proliferative action of purified granulocyte colony-stimulating factor (G-CSF) on normal and leukemic human promyelocytes.
The binding of purified 125I-labeled murine granulocyte colony stimulating factor (125I-G-CSF) to normal and leukemic human cells was examined. Normal neutrophils and their precursors demonstrated specific labeling with 125I-G-CSF, whereas eosinophils, lymphocytes, and erythroid cells did not. Normal human promyelocytes demonstrated the highest binding among hemopoietic cells. Human myeloid leukemic cells also demonstrated consistent specific labeling with 125I-G-CSF. Normal promyelocytes and chronic myeloid leukemia promyelocytes demonstrated only transient clonal proliferation in vitro when stimulated by G-CSF, but this was not always the case with acute promyelocytic leukemic cells. The qualitative responsiveness of normal and leukemic cells to G-CSF was very similar despite heterogeneity in receptor numbers on individual cells. A subset of acute promyelocytic leukemic cells appeared unresponsive to stimulation by GM-CSF.
D000911:Antibodies, Monoclonal; D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D003115:Colony-Stimulating Factors / Q000378:metabolism* / Q000494:pharmacology*; D016179:Granulocyte Colony-Stimulating Factor; D006098:Granulocytes / Q000166:cytology* / Q000378:metabolism; D006133:Growth Substances; D006801:Humans; D007938:Leukemia / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism
D000911:Antibodies, Monoclonal; D003115:Colony-Stimulating Factors; D006133:Growth Substances; D016179:Granulocyte Colony-Stimulating Factor