title: Inhibition of human neutrophil elastase by bacterial polyanions.
abstract: We previously demonstrated that pneumococcal extracts contain a highly specific inhibitor of human neutrophil elastase (HNE). We now show that the active inhibitor in these extracts is a high-molecular-weight, heat-stable substance that appears to be RNA, since inhibitory activity of pneumococcal extracts is decreased by incubation with ribonuclease but not by incubation with deoxyribonuclease or proteinase K. Moreover, metabolically labeled ([3H]uridine) pneumococcal RNA, isolated by phenol extraction followed by ethanol precipitation, strongly inhibits HNE. Pneumococcal capsular polysaccharide, although polyanionic, is only weakly inhibitory toward HNE and is not a major source of elastase-inhibitory activity in pneumococcal extracts. On the other hand, the capsule of Haemophilus influenzae type b contains polyribosylribitol phosphate. This highly charged polyanion possesses HNE-inhibitory activity, but only under special circumstances to be discussed below. Pneumococci (type I, type II smooth, type II rough) and H. influenzae (type b) all release HNE-inhibitory activity into their culture medium during growth. By contrast, Klebsiella pneumoniae and Staphylococcus aureus release little (if any) stable HNE-inhibitory activity during growth. We propose that some bacterial pneumonias may spare host tissue because polyanions released by the invading microorganisms (e.g. RNA from autolysing pneumococci) inhibit elastase released from inflammatory neutrophils and thereby modulate accompanying tissue proteolysis. Pneumonias caused by microorganisms that do not release stable polyanionic inhibitors of HNE (e.g., Staphylococcus and Klebsiella) may be correspondingly more injurious to the lung.
mesh_terms: D006193:Haemophilus influenzae / Q000502:physiology; D006801:Humans; D007711:Klebsiella pneumoniae / Q000502:physiology; D009504:Neutrophils / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011108:Polymers / Q000138:chemical synthesis / Q000494:pharmacology; D011134:Polysaccharides / Q000494:pharmacology; D011135:Polysaccharides, Bacterial / Q000494:pharmacology*; D012329:RNA, Bacterial / Q000494:pharmacology*; D012331:RNA, Fungal / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000502:physiology; D013211:Staphylococcus aureus / Q000502:physiology; D013296:Streptococcus pneumoniae / Q000032:analysis / Q000502:physiology
chemical_list: D011108:Polymers; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; D012329:RNA, Bacterial; D012331:RNA, Fungal; C008984:polyribitol phosphate; D010196:Pancreatic Elastase