title: Surface and species-specific antigens of Schistosoma haematobium.
abstract: Of the surface antigens identified by radio-iodination, two-dimensional gel analyses showed no similarities between those of Schistosoma haematobium and Schistosoma mansoni, thus providing a basis for the species specificity of these antigens described previously (Simpson, Knight, Hagan, Hodgson, Wilkins & Smithers (1985) Parasitology 90, 499-508). The surface antigens of S. haematobium were glycosylated and comprised an acidic polypeptide of Mr 17,000 as well as a complex set of polypeptides of approximate pI 6-7, which resolved in the Mr range 20,000-30,000. At least one of the lower Mr forms of this complex is also present in the adult worm. Limited cross-reaction was observed with S. mansoni infection sera and this may be due to a shared carbohydrate epitope. In contrast, extensive cross-reaction was observed using sera from mice immunized with S. bovis. This pattern parallels the species-specificity of vaccine-induced immunity. Extensive cross-reaction was also observed within cell-free translation products of m-RNA from adult worms of S. haematobium and S. mansoni by use of heterologous human infection sera. The few antigens which were species-specific may represent surface antigens.
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis* / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007118:Immunoassay; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics; D012548:Schistosoma haematobium / Q000235:genetics / Q000276:immunology*; D012550:Schistosoma mansoni / Q000235:genetics / Q000276:immunology; D013045:Species Specificity
chemical_list: D000947:Antigens, Helminth; D000954:Antigens, Surface; D012313:RNA