A misaligned double-stranded RNA, poly(I).poly(C12,U), induces accumulation of 2',5'-oligoadenylates in mouse tissues.
2',5'-Oligoadenylate synthetase was induced 3-2000-fold in spleen, liver, kidney and brain of NIH Swiss mice injected intravenously with 2-200 micrograms of the misaligned dsRNA, poly(I).poly(C12,U). Levels of 2',5'-oligoadenylates extracted from these tissues were also elevated, although the amount of 2',5'-oligoadenylates extracted did not correlate directly with the amount of enzyme present. These results suggest that double-stranded portions of the misaligned polymer survived intracellularly and activated the 2',5'-oligoadenylate synthetase, and that the level of dsRNA may contribute to the control of 2',5'-oligoadenylate metabolism.
D015088:2',5'-Oligoadenylate Synthetase / Q000378:metabolism; D000227:Adenine Nucleotides / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology; D007372:Interferons / Q000097:blood; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D051379:Mice; D009843:Oligoribonucleotides / Q000378:metabolism*; D011070:Poly I-C / Q000494:pharmacology*; D011072:Poly U / Q000494:pharmacology*; D012330:RNA, Double-Stranded / Q000494:pharmacology*; D013154:Spleen / Q000201:enzymology; D014018:Tissue Distribution
D000227:Adenine Nucleotides; D009843:Oligoribonucleotides; D012330:RNA, Double-Stranded; D011072:Poly U; C023505:2',5'-oligoadenylate; D007372:Interferons; C047490:poly(I).poly(c12,U); D015088:2',5'-Oligoadenylate Synthetase; D011070:Poly I-C