In vitro translation of B-cell stimulatory factor-1 in Xenopus laevis oocytes.
B-cell stimulatory factor-1 (BSF-1) can be translated in vitro in Xenopus laevis oocytes. This activity is blocked by an antibody to BSF-1. The RNA species coding for BSF-1 activity sediments of approximately 8-9S and is separable from RNA coding for interleukin-2 activity which sediments at approximately 11.5S. Finally, the fact that BSF-1 can be translated in vitro confirms that functions attributed to BSF-1 do not depend on contamination with other biologically active molecules such as phorbol myristate acetate.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D005260:Female; D006133:Growth Substances / Q000096:biosynthesis / Q000235:genetics*; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4; D008222:Lymphokines / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D009865:Oocytes / Q000378:metabolism; D011061:Poly A / Q000378:metabolism; D014176:Protein Biosynthesis; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014982:Xenopus laevis
D006133:Growth Substances; D007376:Interleukin-2; D008222:Lymphokines; D012333:RNA, Messenger; D015847:Interleukin-4; D011061:Poly A; D012313:RNA; D013755:Tetradecanoylphorbol Acetate