Identification of functional regions in the C-terminal domain of Escherichia coli ribosomal protein S1 using monoclonal antibodies.
Monoclonal antibodies specific for defined regions of E. coli ribosomal protein S1 were used in a R17 mRNA-directed protein synthesis assay to reveal functionally important sites of the protein. Two distinct sites for mRNA binding were identified in the regions 349-437 and 438-547 located in the C-terminal domain of protein S1.
D000911:Antibodies, Monoclonal / Q000276:immunology; D001426:Bacterial Proteins; D001665:Binding Sites; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D004926:Escherichia coli / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012269:Ribosomal Proteins / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D000939:Epitopes; D012333:RNA, Messenger; D012269:Ribosomal Proteins; C015494:ribosomal protein S1