Interactions of retroviral structural proteins with single-stranded nucleic acids.
We have studied the interactions of single-stranded polyribonucleotides with murine leukemia virus structural proteins p10, p10' (a p10 variant), and Pr65gag, as well as Rous sarcoma virus (RSV) pp12 (a p10 analog). Two quantitative assays have been used to monitor protein-RNA association: the fluorescence enhancement of polyethenoadenylic acid) poly(epsilon A) upon binding protein, and tryptophan fluorescence quenching upon binding to poly(U). With each assay p10 was shown to bind stoichiometrically to single-stranded RNA, covering a length of nucleic acid chain (occluded site size, n) of about 6 residues. RSV pp12 was also shown to bind to poly(epsilon A), with n = 5 +/- 1. Addition of NaCl to fully titrated MuLV p10-nucleic acid mixtures effected nearly complete restoration of poly(epsilon A) or MuLV p10 fluorescence. Under conditions of 0.06 M NaCl, p10 bound noncooperatively to poly(epsilon A) with an intrinsic association constant, K = 2.3 X 10(6) M-1. K and n determined in this study were shown to relate to Kapp determined by other methods, by the approximation Kapp approximately NK, where N is the number of binding sites along the polynucleotide chain ((nucleotides/chain)/n). Chemical modifications of the p10 cysteine residues did not alter the affinity for poly(epsilon A). The affinity of Pr65gag for poly(epsilon A) appears to be higher than that of p10.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004277:DNA, Single-Stranded / Q000378:metabolism*; D007700:Kinetics; D008433:Mathematics; D011061:Poly A / Q000378:metabolism; D011072:Poly U / Q000378:metabolism; D012313:RNA / Q000378:metabolism*; D012190:Retroviridae / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*
D004277:DNA, Single-Stranded; D014764:Viral Proteins; D011061:Poly A; D011072:Poly U; C005163:poly(1,N(6)-ethenoadenylic acid); D012313:RNA