Secretion and role of interleukin-1-like factor in the antibody response to dinitrophenyl dextran and other type 2 T-independent antigens.
Splenic adherent cells (SAC) were found to produce a humoral factor when they were cultured with dinitrophenyl-dextran or some other type 2 T-independent (TI-2) antigens. The factor substituted adherent cells in in vitro antibody responses to TI-2 antigens, and acted in an antigen-nonspecific and H-2-nonrestricted manner. T cells were indicated not to participate in the production of the factor. The factor was eluted from a Sephadex G-75 column with interleukin-1 (IL-1) activity to promote a thymocyte proliferation response to phytohemagglutinin. The molecular weight of the factor was estimated to be 16,000 Da. Both activities of the factor were absorbed by LBRM-33-1A5 cells. These results indicate that SAC secrete IL-1-like factor on direct stimulation by TI-2 antigens and that the secretion of the factor represents a major function of SAC in the antibody response to these antigens.
D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology*; D002478:Cells, Cultured; D003911:Dextrans / Q000276:immunology; D004136:Dinitrobenzenes / Q000276:immunology; D006183:H-2 Antigens / Q000235:genetics; D007375:Interleukin-1 / Q000032:analysis / Q000502:physiology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000502:physiology
D000941:Antigens; D003911:Dextrans; D004136:Dinitrobenzenes; D006183:H-2 Antigens; D007375:Interleukin-1; D008070:Lipopolysaccharides