Cross-blot and cross-dot system: a high-performance system for the detection of antigen-antibody complexes on nitrocellulose.
We present a reliable, simple, and quick system for screening antibody-antigen complexes on nitrocellulose. The apparatus necessary for this system is inexpensive and easy to use, and it can be adapted to blot or dot analysis without any modification. The number of antibody-antigen combinations that can be tested in one experiment ranges from 25 to 31 for blot analysis and from 345 to 600 for dot analysis. This system also offers numerous experimental advantages: it makes it possible to estimate with only one experiment the contribution of the different reaction stages to background noise and so allows unambiguous interpretation of the antibody-antigen reaction. Furthermore, this system can be used for any hybridization experiment on nitrocellulose.
D000818:Animals; D000936:Antigen-Antibody Complex / Q000032:analysis*; D002417:Cattle; D003101:Collodion; D004331:Drosophila melanogaster; D003576:Electron Transport Complex IV / Q000276:immunology; D004586:Electrophoresis; D007120:Immunochemistry / Q000295:instrumentation; D008565:Membrane Proteins / Q000276:immunology; D008928:Mitochondria / Q000276:immunology; D013194:Staining and Labeling; D013552:Swine
D000936:Antigen-Antibody Complex; D008565:Membrane Proteins; D003101:Collodion; D003576:Electron Transport Complex IV