Hydrophobic interaction chromatography of incompletely methylated transfer RNA from Escherichia coli on octyl-sepharose.
Phenylalanine-specific transfer RNA from methionine-starved relaxed Escherichia coli K12 separates into two components when chromatographed on Octyl-Sepharose. The difference in elution between the two tRNAs has been shown to depend on the methyl group in the highly modified 2-methylthio-N-6-isopentenyladenosine. The first eluted tRNAPhe lacks this methyl group, while the last eluted tRNAPhe is fully methylated. Other differences in the modification patterns have no effect on the elution from Octyl-Sepharose. The elution pattern of tyrosine- and serine-specific tRNAs, also normally containing ms2i6A, is similar.
D000604:Amino Acyl-tRNA Synthetases / Q000032:analysis; D002852:Chromatography, Ion Exchange; D004926:Escherichia coli / Q000032:analysis*; D010084:Oxidation-Reduction; D012329:RNA, Bacterial / Q000032:analysis*; D012343:RNA, Transfer / Q000032:analysis*; D012685:Sepharose / Q000031:analogs & derivatives
D012329:RNA, Bacterial; C027176:octyl-sepharose CL-4B; D012685:Sepharose; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases