Monoclonal antibody directed to a Hanganutziu-Deicher active ganglioside, GM2 (NeuGc).
Spleen cells from NZB mouse immunized with a membrane fraction of rabbit thymus tissue were fused with BALB/c 6-thioguanine-resistant myeloma cells, P3-X63-Ag8.653. One hybridoma clone (Y-2-HD-1) produced IgM immunoglobulin that bound to an N-glycolylneuraminic acid-containing GM2 ganglioside, GM2(NeuGc), which is known to be a Hanganutziu-Deicher antigen. The specificity of the Y-2-HD-1 monoclonal antibody was examined, using authentic glycosphingolipids structurally related to GM2(NeuGc), by means of an enzyme-linked immunosorbent assay and thin-layer chromatography/enzyme immunostaining, respectively. The monoclonal antibody was found to be highly specific to GM2(NeuGc) and the epitope was a non-reducing terminal GalNAc beta 1-4[NeuGc alpha 2-3]Gal structure. This monoclonal antibody (Y-2-HD-1) bound to native mouse erythrocytes, in which GM2(NeuGc) is a major ganglioside. These results indicate that GM2(NeuGc) is located on the surface of mouse erythrocytes.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D015478:Antigens, Heterophile / Q000276:immunology*; D001095:Arachidonic Acids / Q000096:biosynthesis; D002855:Chromatography, Thin Layer; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis; D005260:Female; D005678:G(M2) Ganglioside / Q000031:analogs & derivatives / Q000276:immunology*; D005732:Gangliosides / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D007124:Immunoenzyme Techniques; D007075:Immunoglobulin M / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008814:Mice, Inbred NZB; D011817:Rabbits
C054994:12-keto-5,8,11,13-eicosatetraenoic acid; D000911:Antibodies, Monoclonal; D015478:Antigens, Heterophile; D001095:Arachidonic Acids; D000939:Epitopes; D005231:Fatty Acids, Unsaturated; D005732:Gangliosides; D007075:Immunoglobulin M; D005678:G(M2) Ganglioside; C054992:15-keto-5,8,11,13-eicosatetraenoic acid; C045702:GM2 N-glycolylneuraminic acid ganglioside