Tolerance induction by continuous stimulation with dextran B512.
The thymus-independent antigen Dextran B512 was used to induce tolerance with immunogenic doses. We show the difficulty to obtain a steady state by administering weekly antigen doses. Three weekly doses were required to achieve tolerance or "homeostatic equilibrium". Three groups of mice (C57BL6 X CBA) F1 were stimulated with: a) 0.5 microgram Dextran one weekly dose for ten weeks. b) 2 micrograms Dex. one weekly dose, for twenty-two weeks, and c) 10 micrograms Dex. three times a week, administered continuously for eleven weeks. The number of anti-Dex. antibody secreting cells was determined by Cunningham and Szenberg PFC technique. Weekly continuous doses of 0.5 and 2 micrograms of antigen showed frequent fluctuations in IgM and unresponsiveness was not achieved. Nonetheless, a three times weekly dose of 10 micrograms Dex. showed IgM response with oscillations and little anti-Dex. IgG response was obtained. After eleven weeks of continuous stimulation the steady state was succeeded. This antigen triggered high levels of IgM bearing B cells in mice, and more difficult tolerance induction was known in this isotype, but also the stimulation model was an important fact in achievement of steady state.
D000818:Animals; D000955:Antigens, T-Independent / Q000276:immunology*; D003911:Dextrans / Q000008:administration & dosage / Q000276:immunology*; D007108:Immune Tolerance; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL / Q000276:immunology; D008808:Mice, Inbred CBA / Q000276:immunology
D000955:Antigens, T-Independent; D003911:Dextrans; D007074:Immunoglobulin G; D007075:Immunoglobulin M