Histamine- and serotonin-releasing activity of the supernatants from guinea pig spleen cell cultures.
The action of supernatants from cultivated in vitro guinea pig spleen cells on the mast cells of guinea pigs, rats and hamsters was studied. It was found that supernatants from guinea pig spleen cell cultures are potent to release histamine from mast cells of the examined populations in a dose-dependent fashion. Histamine release from heterologous mast cells (especially from rat pleural mast cells) was significantly higher than that from homologous mesenteric mast cells. It was also demonstrated (on rat mast cells) that guinea pig spleen cell supernatants possessed not only histamine but 5-hydroxytryptamine releasing activity as well. Rat pleural mast cells were more sensitive and released more serotonin after challenge with spleen cell supernatants than peritoneal cells.
D000818:Animals; D014408:Biomarkers, Tumor; D002478:Cells, Cultured; D006224:Cricetinae; D006168:Guinea Pigs; D006636:Histamine Release / Q000187:drug effects*; D008214:Lymphocytes / Q000276:immunology; D008222:Lymphokines / Q000494:pharmacology*; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D012701:Serotonin / Q000378:metabolism*; D013045:Species Specificity; D013154:Spleen / Q000276:immunology; D000090324:Tumor Protein, Translationally-Controlled 1
D014408:Biomarkers, Tumor; D008222:Lymphokines; D000090324:Tumor Protein, Translationally-Controlled 1; D012701:Serotonin