Novobiocin inhibits Xenopus transcription factor IIIA-DNA interactions.
Novobiocin and coumermycin, inhibitors of the B subunit of Escherichia coli DNA gyrase, inhibit the binding of Xenopus transcription factor IIIA (TFIIIA) to the 5 S RNA gene. Nalidixic acid and oxolinic acid, inhibitors of the A subunit of DNA gyrase, have no effect on the TFIIIA-5 S RNA gene interaction. Novobiocin and coumermycin inhibit TFIIIA-dependent DNA renaturation. Novobiocin dissociates TFIIIA.5 S RNA gene complexes and TFIIIA.5 S RNA complexes (7 S particles). Novobiocin induces TFIIIA aggregation, a phenomenon likely to be responsible for the inhibition of TFIIIA-DNA interactions. Novobiocin inhibition of TFIIIA can be reversed by dilution.
D049933:Aminocoumarins; D000818:Animals; D003374:Coumarins / Q000494:pharmacology; D004247:DNA / Q000378:metabolism*; D003850:Deoxyribonuclease I / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D009268:Nalidixic Acid / Q000494:pharmacology; D009675:Novobiocin / Q000494:pharmacology*; D009695:Nucleic Acid Renaturation; D010093:Oxolinic Acid / Q000494:pharmacology; D012313:RNA / Q000378:metabolism; D059005:Topoisomerase II Inhibitors; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014982:Xenopus laevis
D049933:Aminocoumarins; D003374:Coumarins; D059005:Topoisomerase II Inhibitors; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors; D009675:Novobiocin; D009268:Nalidixic Acid; D012313:RNA; D004247:DNA; D003850:Deoxyribonuclease I; D010093:Oxolinic Acid; C004628:coumermycin