title: Glycoproteins specific for the retinal rod outer segment plasma membrane.
abstract: Two ricin-specific glycoproteins have been identified on neuraminidase-treated rod outer segment plasma membranes of bovine retinal photoreceptor cells. Ricin-gold-dextran particles were observed by electron microscopy to densely label the surface of neuraminidase-treated rod outer segments. Western blotting of proteins separated by SDS-gel electrophoresis indicated that two ricin-binding glycoproteins of Mr 230,000 and 110,000 are specific for the plasma membrane and are not found in disk membranes. These glycoproteins can serve as specific probes for the purification of the rod outer segment plasma membrane.
mesh_terms: D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000032:analysis*; D003911:Dextrans; D006023:Glycoproteins / Q000032:analysis*; D006046:Gold; D006651:Histocytochemistry; D008854:Microscopy, Electron; D008970:Molecular Weight; D009439:Neuraminidase / Q000378:metabolism; D010786:Photoreceptor Cells / Q000648:ultrastructure*; D012276:Ricin; D012374:Rod Cell Outer Segment / Q000648:ultrastructure*
chemical_list: D003911:Dextrans; D006023:Glycoproteins; D006046:Gold; D012276:Ricin; D009439:Neuraminidase