Clonal analysis of T cell responses to herpes simplex virus: a HSV-specific, non cytolytic T cell clone displays specific helper activity for T cell response in vitro.
A HSV-specific, Lyt 1+2- T cell clone (D7.1) was established by repetitive in vitro restimulation of in vivo HSV-1 primed draining lymph node cells with UV-inactivated homologous virus. Earlier work had shown that this clone could provide specific helper activity for HSV-1 primed B cells for antibody production in vivo. In this paper, further functional analysis has demonstrated that this clone and its subclones could also help unprimed T cells in the generation of effector DTH T cells in vitro. The helper activity was found to be antigen-specific and cell dose-dependent. In addition, this helper T cell clone showed little, if any, natural killer-like activity and was non-cytolytic towards both HSV-infected and uninfected syngeneic targets. However, they could exert potent cytotoxic activity towards P815 and EL-4 tumour targets in the presence of lectins.
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D000939:Epitopes / Q000276:immunology; D005260:Female; D006561:Herpes Simplex / Q000276:immunology; D006968:Hypersensitivity, Delayed / Q000276:immunology; D037102:Lectins / Q000276:immunology / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D018139:Simplexvirus / Q000276:immunology*; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology* / Q000502:physiology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000502:physiology*
D000956:Antigens, Viral; D000939:Epitopes; D037102:Lectins