Genetic reassortment between two human rotaviruses having different serotype and subgroup specificities.
Two reassortant viruses were selected from a mixed infection of MA104 cells with human rotavirus strains Wa (serotype 1-subgroup II) and HN126 (serotype 2-subgroup I). Antigenic characterization and genotype analysis by polyacrylamide gel electrophoresis revealed that they were reassortants with novel antigenic compositions, i.e. serotype 1-subgroup I (C116) and serotype 2-subgroup II (C15). Furthermore one of them, C15, was considered to have a mosaic antigenicity defined by two serotype-specific antigens, namely the serotype 1-specific VP3 antigen and the serotype 2-specific VP7 antigen. Although this reassortant was shown to be a serotype 2 virus on the basis of its preferential reactivity in neutralization reactions with serotype 2 antiserum, unexpectedly the antiserum prepared against C15 equally neutralized both serotype 1 and serotype 2 strains.
D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D000939:Epitopes; D005838:Genotype; D006801:Humans; D007106:Immune Sera; D009500:Neutralization Tests; D011995:Recombination, Genetic; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000276:immunology; D012703:Serotyping; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology; D015678:Viral Structural Proteins
D000956:Antigens, Viral; D000939:Epitopes; D007106:Immune Sera; D014764:Viral Proteins; D015678:Viral Structural Proteins