title: A mammalian mitochondrial RNA processing activity contains nucleus-encoded RNA.
abstract: Ribonuclease mitochondrial RNA processing, a site-specific endoribonuclease involved in primer RNA metabolism in mammalian mitochondria, requires an RNA component for its activity. On the basis of copurification and selective inactivation with complementary oligonucleotides, a 135-nucleotide RNA species, not encoded in the mitochondrial genome, is identified as the RNA moiety of the endoribonuclease. This finding implies transport of a nucleus-encoded RNA, essential for organelle DNA replication, to the mitochondrial matrix.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000502:physiology*; D055598:Chemical Phenomena; D002621:Chemistry; D004351:Drug Resistance; D004720:Endonucleases / Q000302:isolation & purification / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D005815:Genetic Code; D006801:Humans; D008322:Mammals / Q000235:genetics* / Q000378:metabolism; D008836:Micrococcal Nuclease / Q000494:pharmacology; D008928:Mitochondria / Q000378:metabolism*; D009841:Oligonucleotides / Q000494:pharmacology; D009940:Organoids / Q000502:physiology; D012313:RNA / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D012260:Ribonucleases / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D009841:Oligonucleotides; D012313:RNA; D004720:Endonucleases; D012260:Ribonucleases; D008836:Micrococcal Nuclease