title: In vitro carboxymethylation of myelin basic protein.
abstract: During a study to find natural substrate proteins of carboxymethylation, myelin basic protein was found to be a good substrate. The two protein carboxymethylases were purified partially using myelin basic protein as a substrate. These two enzymes may be identical with protein carboxymethylase I and II, which have been found to methylate gamma-globulin. The Km of myelin basic protein (25 microM) was very small compared with other substrates. The activities of the two carboxymethylases were high in the rat brain in comparison to the other rat organs. The activity increased during the period of myelination in the rat brain. These findings suggest that carboxymethylation of myelin basic protein may play an important role in myelination.
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D008168:Lung / Q000201:enzymology; D008297:Male; D004676:Myelin Basic Protein / Q000378:metabolism*; D009206:Myocardium / Q000201:enzymology; D011496:Protein Methyltransferases / Q000378:metabolism*; D011497:Protein O-Methyltransferase / Q000145:classification / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013379:Substrate Specificity; D013737:Testis / Q000201:enzymology
chemical_list: D004676:Myelin Basic Protein; D011496:Protein Methyltransferases; D011497:Protein O-Methyltransferase