title: In vivo labelling and axonal transport of monoamine oxidase in the rat basal ganglia using radioactive pargyline.
abstract: The enzyme monoamine oxidase was labelled in the rat striatum or substantia nigra with locally injected radioactive pargyline. The binding was prevented by a pretreatment with non-radioactive pargyline, or with a combination of clorgyline and deprenyl. Most of the MAO labelled with 3H-pargyline was of the B-type, but also some MAO-A was labelled, as shown in rats pretreated with clorgyline or deprenyl separately. Seven days after the injection of (3H)-pargyline into the striatum a significant labelling was observed in the substantia nigra. This labelling was clorgyline sensitive, indicating type A MAO, and was not present when striatal neurons were destroyed with kainic acid. Labelling of the striatum following 3H-pargyline injection into the substantia nigra was also less in kainate intoxicated striata. Damage of nigral dopamine neurons with 6-hydroxydopamine did not influence the distribution of the label. Thus by using 3H-pargyline, specific labelling and axonal transport of type A MAO in striatal neurons projecting to the substantia nigra was demonstrated.
mesh_terms: D000818:Animals; D001370:Axonal Transport / Q000187:drug effects; D001479:Basal Ganglia / Q000201:enzymology*; D003010:Clorgyline / Q000494:pharmacology; D003342:Corpus Striatum / Q000201:enzymology; D005260:Female; D006892:Hydroxydopamines / Q000494:pharmacology; D007608:Kainic Acid; D008297:Male; D008995:Monoamine Oxidase / Q000378:metabolism* / Q000502:physiology; D016627:Oxidopamine; D010293:Pargyline; D011292:Premedication; D051381:Rats; D011919:Rats, Inbred Strains; D012642:Selegiline / Q000494:pharmacology; D013378:Substantia Nigra / Q000187:drug effects
chemical_list: D006892:Hydroxydopamines; D012642:Selegiline; D016627:Oxidopamine; D010293:Pargyline; D008995:Monoamine Oxidase; D003010:Clorgyline; D007608:Kainic Acid