In vivo suppression of perinatal multispecific B cells results in a distortion of the adult B cell repertoire.
The isolation of multispecific B cell hybridomas with a variety of anti-idiotype (anti-Id) activities from the lymphoid organs of fetal and neonatal BALB/c mice suggested that the development of the immune system may depend on Id interactions among autologous B cells. In vitro analysis of antibodies secreted by these hybridomas showed extensive sharing of an idiotope defined by the monoclonal antibody FD5-1. Early and timed administration of this antibody during the perinatal period results in a distortion of the phosphorylcholine (PC) and alpha (1----3)dextran (Dex)-specific B cell precursor compartment of the developing repertoire and is reflected by a drastic reduction of antibody responses to these antigens when challenged as adults. These observations provide strong evidence for the involvement of the early appearing multispecific B cells in Id interactions that bring about the uniform development of the normal adult B cell repertoire. Interference with these interactions at critical stages of developmental results in permanent deficiencies in the adult B cell repertoire.
D000818:Animals; D000831:Animals, Newborn / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D001323:Autoantibodies / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D003911:Dextrans / Q000276:immunology; D005333:Fetus / Q000276:immunology*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology; D007135:Immunoglobulin Variable Region / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D010449:Peptide Mapping; D010767:Phosphorylcholine / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014301:Trinitrobenzenes / Q000276:immunology
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D003911:Dextrans; D007143:Immunoglobulin Heavy Chains; D007130:Immunoglobulin Idiotypes; D007075:Immunoglobulin M; D007135:Immunoglobulin Variable Region; D014301:Trinitrobenzenes; D010767:Phosphorylcholine