Modified technique for cytochrome oxidase histochemistry: increased staining intensity and compatibility with 2-deoxyglucose autoradiography.
Staining the brain for cytochrome oxidase (CO) produces patterns which can resemble the patterns produced by 2-deoxyglucose (2-DG) autoradiography. In order to assess the differences between CO patterns of long-term metabolic activity and 2-DG patterns of short-term activity, comparisons should, ideally, be made on the same section. Consequently, we have made certain modifications in the standard CO histologic procedure which improve the stain and allow both 2-DG autoradiography and CO staining on the same section.
D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000201:enzymology* / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism; D003837:Deoxy Sugars / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism*; D006651:Histocytochemistry / Q000379:methods*; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling / Q000379:methods
D003837:Deoxy Sugars; D003847:Deoxyglucose; D003576:Electron Transport Complex IV