Localization of pancreatic enzyme secretion-stimulating activity and trypsin inhibitory activity in zymogen granule of the rat pancreas.
The intracellular localization of pancreatic enzyme secretion-stimulating activity in rat pancreas was investigated. We found and purified a pancreatic enzyme secretion-stimulating peptide from rat bile/pancreatic juice. The peptide is trypsin-sensitive (showing temporary trypsin inhibitory activity), and it is hypothesized that it acts as a trypsin-sensitive mediator in the feedback regulation of diet-induced pancreatic enzyme secretion. The zymogen granule fraction was purified 5-fold by ultracentrifugation by the Percoll density gradient method. The purity of the zymogen granule fraction was determined from the specific amylase activity and electron microscopic morphology. The specific enzyme activities of amylase and trypsin and the trypsin inhibitory activity increased in parallel during the purification, and the pancreatic enzyme secretion-stimulating activity was also localized in the zymogen granule fraction. These results suggest that the pancreatic enzyme secretion-stimulating peptide originates from the acinar cells, and that it is secreted through exocytosis of zymogen granules into the small intestine, its ratio to trypsin thus remaining constant. This idea supports our hypothesis that the stimulating peptide acts as a mediator for the feedback regulation of pancreatic enzyme secretion by trypsin.
D000681:Amylases / Q000302:isolation & purification; D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D003594:Cytoplasmic Granules / Q000201:enzymology / Q000378:metabolism*; D004792:Enzyme Precursors / Q000378:metabolism*; D008297:Male; D010179:Pancreas / Q000201:enzymology* / Q000378:metabolism; D010455:Peptides / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains; D014357:Trypsin / Q000302:isolation & purification; D014359:Trypsin Inhibitor, Kazal Pancreatic / Q000302:isolation & purification*; D014361:Trypsin Inhibitors / Q000302:isolation & purification*
D004792:Enzyme Precursors; D010455:Peptides; D014361:Trypsin Inhibitors; D014359:Trypsin Inhibitor, Kazal Pancreatic; D000681:Amylases; D014357:Trypsin