Characterization of primate bronchoalveolar mast cells. I. IgE-dependent release of histamine, leukotrienes, and prostaglandins.
Large numbers of functional mast cells were obtained by bronchoalveolar lavage (BAL) of Macaca arctoides monkeys that had been infected with the nematode Ascaris suum. These lavage cells, of which 21% were mast cells, released histamine, LTC4, and PGD2 in a concentration-dependent fashion when challenged with ascaris antigen or antibody to human IgE. However, there was no release of histamine when these cells were challenged with compound 48/80. The amount of mediator released was highly dependent on the sensitivity of the cells to immunologic challenge, but was generally in the range of 2 to 5 micrograms histamine (30 to 70% of total), 20 to 80 ng LTC4, and 100 to 300 ng PGD2 per 10(6) mast cells when maximally challenged. Other eicosanoids measured were released only in much smaller quantities. Maximal values were 4 ng LTB4, 2 ng PGE2, and approximately 10 to 20 ng PGF2 alpha per 10(6) mast cells. The amount of LTC4 and PGD2 released correlated with the release of histamine, the calculated regression line indicating that 18 ng LTC4 and 50 ng PGD2 were released per microgram of histamine released. This correlation suggests that the majority of the LTC4 and PGD2 released was probably mast cell-derived. Further support for this conclusion was given by the observation that when lavage cells were fractioned on continuous Percoll gradients, the ability to release LTC4 and PGD2 on immunologic challenge coincided with the peak of mast cells.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D001196:Ascariasis / Q000473:pathology; D001980:Bronchi / Q000473:pathology; D015232:Dinoprostone; D006636:Histamine Release; D007073:Immunoglobulin E / Q000276:immunology*; D007975:Leukotriene B4 / Q000378:metabolism; D008251:Macaca / Q000276:immunology*; D008407:Mast Cells / Q000378:metabolism*; D015230:Prostaglandin D2; D011457:Prostaglandins D / Q000378:metabolism*; D011458:Prostaglandins E / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000473:pathology; D013189:SRS-A / Q000378:metabolism*; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D000888:Antibodies, Anti-Idiotypic; D011457:Prostaglandins D; D011458:Prostaglandins E; D013189:SRS-A; C026408:anti-IgE antibodies; D007975:Leukotriene B4; D007073:Immunoglobulin E; D003189:p-Methoxy-N-methylphenethylamine; D015232:Dinoprostone; D015230:Prostaglandin D2