title: Alteration of protein phosphorylation patterns in cell lines morphologically transformed by human cytomegalovirus.
abstract: Human fibroblastic cell lines morphologically transformed by either live virus or DNA fragments of human cytomegalovirus had altered plasma membrane protein composition; quantitative changes, and gains and losses in protein composition in comparison to normal parent cell lines were detected. These transformed cell lines showed altered total cell protein phosphorylation patterns when compared to parent cell lines. A two to four fold increase in in vivo protein phosphorylation at serine and threonine residues was observed; no increase in phosphorylation at total cell tyrosine residues was detected. Analysis of the in vivo phosphorylated protein by two dimensional gel electrophoresis revealed some similarities as well as differences in the types of polypeptides phosphorylated between transformed and control cell lines. Increased (two-to sixfold over parent cell extracts) casein kinase and polyamine dependent casein kinase activities were detected in HCMV transformed cell extracts.
mesh_terms: D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D003587:Cytomegalovirus; D006801:Humans; D007526:Isoelectric Point; D008565:Membrane Proteins / Q000378:metabolism; D008970:Molecular Weight; D010750:Phosphoproteins / Q000378:metabolism*; D010768:Phosphoserine / Q000378:metabolism; D019000:Phosphotyrosine; D011494:Protein Kinases / Q000378:metabolism; D013095:Spermidine / Q000494:pharmacology; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D010750:Phosphoproteins; D010768:Phosphoserine; D019000:Phosphotyrosine; D014443:Tyrosine; D011494:Protein Kinases; D013095:Spermidine