title: The use of surface spread polytene chromosomes for high resolution fluorescence microscopic analyses.
abstract: The technique of surface spreading of polytene chromosomes is applied to fluorescence microscopy. With bisbenzimide Hoechst 33258 stained surface spread polytene chromosomes from the dipteran species Chironomus thummi piger, depiction of the band-interband structure is close to that of electron micrographs of the same enlargement.
mesh_terms: D000818:Animals; D006690:Bisbenzimidazole; D002683:Chironomidae / Q000235:genetics*; D002871:Chromosome Banding / Q000379:methods*; D004175:Diptera / Q000235:genetics*; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence
chemical_list: D006690:Bisbenzimidazole