[Detection of cationic proteins and peroxidase in semithin sections].
A method of histochemical identification of cationic proteins, peroxidase, viable and killed bacteria in semithin sections of biopsy samples is suggested. The method is based on dying of araldite-embedded semithin sections with a buffered alcohol solution of fast green (pH 8.15) and on peroxidase identification with O-dianisidine. The method suggested can be used for distinguishing occult forms of infectious gastritis, evaluation of secretory and barrier function of gastric epithelium, selective dying of leucocytes in tissues, revealing of viable and non-viable bacteria in the infective foci.
D000328:Adult; D001419:Bacteria / Q000378:metabolism; D001706:Biopsy; D003962:Dianisidine; D004853:Epoxy Resins; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D006651:Histocytochemistry; D006801:Humans; D010544:Peroxidases / Q000378:metabolism*; D010796:Phthalic Anhydrides; D011506:Proteins / Q000378:metabolism*; D013194:Staining and Labeling / Q000379:methods; D013270:Stomach / Q000473:pathology
D004853:Epoxy Resins; D010796:Phthalic Anhydrides; D011506:Proteins; C005752:araldite; D010544:Peroxidases; D003962:Dianisidine
Vyiavlenie kationnykh belkov i peroksidazy na polutonkikh srezakh.