Activation of the human neutrophil respiratory burst by an anion channel blocker.
We found that 4,4'-diisothiocyano-2,2'-disulfonic acid (DIDS), an anion channel blocker in erythrocytes, caused a concentration-related stimulation of oxygen radicals in human neutrophils, as measured by luminol- and lucigenin-amplified chemiluminescence. The DIDS-elicited oxygen radical burst followed the kinetic pattern of other stimuli with a lag period of 20 seconds, reaching a maximum after 12 to 15 minutes. Washing neutrophils that had been pretreated with DIDS did not reverse neutrophil activation. DIDS was found to strongly stimulate lactate production and did not block the efflux of this anion. Cytochalasin B completely abolished the chemiluminescence responses when added before DIDS stimulation. It also inhibited lactate production, however, only in a glucose-containing medium. Modulation of oxygen radical production by DIDS may reveal an additional transductional pathway for neutrophil activation.
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology*; D000838:Anions / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism / Q000502:physiology; D003571:Cytochalasin B / Q000494:pharmacology; D005110:Extracellular Space / Q000378:metabolism; D006801:Humans; D007473:Ion Channels / Q000187:drug effects*; D008163:Luminescent Measurements; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000378:metabolism*; D010101:Oxygen Consumption; D013267:Stilbenes / Q000494:pharmacology*
D000838:Anions; D007473:Ion Channels; D013267:Stilbenes; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D003571:Cytochalasin B; D009240:N-Formylmethionine Leucyl-Phenylalanine; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D002118:Calcium