Cell-mediated tumor-killing effect studied by using bilayer lipid membranes.
The bilayer lipid membrane (BLM) system was used to investigate the tumor killing effect of natural killer (NK) cells under various experimental conditions. It was found that NK cells interact specifically with BLMs made from lipids and proteolipids isolated from target K562 cells inducing an increase of the membrane conductance. This effect was more pronounced when the NK cells were pretreated with interferon. A similar effect was observed when NK cells were pretreated with sodium selenite. The results suggest that changes in membrane conductance and permeability are involved in the mechanism of the tumor-killing effect mediated by NK cells.
D002462:Cell Membrane / Q000502:physiology; D003602:Cytotoxicity, Immunologic; D004553:Electric Conductivity; D006801:Humans; D007111:Immunity, Cellular; D007113:Immunity, Innate; D066298:In Vitro Techniques; D007372:Interferons / Q000494:pharmacology; D007473:Ion Channels / Q000502:physiology; D007694:Killer Cells, Natural / Q000502:physiology*; D008051:Lipid Bilayers; D012643:Selenium / Q000494:pharmacology
D007473:Ion Channels; D008051:Lipid Bilayers; D007372:Interferons; D012643:Selenium