[Histamine liberation from rat mast cells by culture supernatants of human lymphocytes and rat spleen lymphocytes].
The supernatant of mitogen- or antigen-stimulated mononuclear cell cultures is known to contain a large number of biologically active molecules. In the present study, we have stimulated human mononuclear cells and rat spleen cells with Con A or antigen (PPD) respectively to produce lymphokines, such as histamine releasing factor (HRF), which is capable of causing histamine release from rat mast cells and human basophils. Histamine was measured fluorimetrically by Shore et al. This assay is sensitive and reproducible: replicates varied by less than 15% in triplicate samples.
D000328:Adult; D000818:Animals; D014408:Biomarkers, Tumor; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media / Q000032:analysis; D006636:Histamine Release; D000949:Histocompatibility Antigens Class II / Q000008:administration & dosage / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D008222:Lymphokines / Q000096:biosynthesis; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000166:cytology; D013997:Time Factors; D000090324:Tumor Protein, Translationally-Controlled 1
D014408:Biomarkers, Tumor; D003470:Culture Media; D000949:Histocompatibility Antigens Class II; D008222:Lymphokines; D000090324:Tumor Protein, Translationally-Controlled 1; D003208:Concanavalin A
Histaminfreisetzung aus Rattenmastzellen, hervorgerufen durch Kulturüberstände menschlicher Lymphozyten und von Rattenmilzlymphozyten.