title: Modified method of silver staining of proteins in polyacrylamide gels.
abstract: The very sensitive and reliable silver staining method to visualize proteins in polyacrylamide gels described by Wray et al. (Anal. Biochem. (1981) 118, 197-203) fails when the protein sample contains nucleic acids and/or metals. By washing the polyacrylamide gels in acetic acid and repeatedly in methanol immediately following electrophoresis and then using the procedure of Wray et al., many gels otherwise unstainable may be stained with a high degree of reliability. This method allows visualization of a minute amount of proteins in samples containing high amounts of DNA and metals.
mesh_terms: D002460:Cell Line; D003432:Cross-Linking Reagents; D004247:DNA; D004591:Electrophoresis, Polyacrylamide Gel; D008670:Metals; D011506:Proteins / Q000032:analysis*; D012834:Silver; D013194:Staining and Labeling
chemical_list: D003432:Cross-Linking Reagents; D008670:Metals; D011506:Proteins; D012834:Silver; D004247:DNA