Location of the epitope for the alpha-tubulin monoclonal antibody TU-O1.
A cyanogen bromide peptide of pig brain alpha-tubulin with high reactivity to the monoclonal antibody TU-O1 has been isolated and identified. It corresponds to positions 37-154 of the alpha-tubulin sequence. A tryptic peptide within this region corresponding to positions 65-79 was also immunoreactive. Its relatively low reactivity, however, indicates that one or more important determinants are missing.
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid; D003488:Cyanogen Bromide; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D010449:Peptide Mapping; D013552:Swine; D014404:Tubulin / Q000032:analysis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D014404:Tubulin; D003488:Cyanogen Bromide