Nutrition and somatomedin. XVI: Somatomedins and somatomedin inhibitors in fasted and refed rats.
Nutritional deprivation is associated with poor growth and decreased levels of net circulating somatomedin activity, as measured by bioassay. Since somatomedin activity reflects the contributions both of somatomedins (which stimulate cartilage) and of somatomedin inhibitors (which antagonize the ability of the somatomedins to stimulate cartilage), we asked if changes in net somatomedin activity could involve progressive underlying alterations in levels of both somatomedins and somatomedin inhibitors. Groups of rats were killed during three days of fasting and 24 hours of refeeding. Fasting was associated with a rise in serum beta-hydroxybutyrate from 1.6 to 12.6 mmol/L after one day, followed by a decline to 4 mmol/L at three days. Somatomedins (low-MW) were separated from somatomedin inhibitors (high-MW) by gel permeation chromatography at acid pH on Sephadex G-50 and TSK-2000 HPLC. Somatomedins fell 35% after one day of fasting, and decreased to 59% below control levels after three days (P less than .05). Somatomedins did not change with six hours of refeeding, but then rose rapidly, reaching control levels after 24 hours. Somatomedins were correlated with change in weight (r = .41, P less than .05), but not with glucose or beta-hydroxybutyrate. Inhibitors rose to 195% above control-levels after one day of fasting, and continued to rise to 375% above control after three days (P less than .01). In contrast to the delayed change in somatomedins with refeeding, there was an abrupt fall in inhibitors (41% below three-day fasted levels after six hours), returning to control levels after 24 hours.(ABSTRACT TRUNCATED AT 250 WORDS)
D020155:3-Hydroxybutyric Acid; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002352:Carrier Proteins / Q000097:blood; D005215:Fasting; D006706:Homeostasis; D006885:Hydroxybutyrates / Q000097:blood; D018969:Insulin-Like Growth Factor Binding Proteins; D008099:Liver / Q000378:metabolism; D008297:Male; D009752:Nutritional Status; D051381:Rats; D011919:Rats, Inbred Strains; D013002:Somatomedins / Q000097:blood*
D001786:Blood Glucose; D002352:Carrier Proteins; D006885:Hydroxybutyrates; D018969:Insulin-Like Growth Factor Binding Proteins; D013002:Somatomedins; C021250:somatomedin inhibitor; D020155:3-Hydroxybutyric Acid