title: Structural protein transport in elongating motor axons after sciatic nerve crush. Effect of a conditioning lesion.
abstract: In elongating motor axons of the rat sciatic nerve, the maximum outgrowth rate increased from 4.6 to 5.3 mm/d (5.3-6.1 X 10(-8) m/s) when a testing lesion of spinal nerves L4 and L5 was preceded 2 wk earlier by a conditioning lesion of the sciatic nerve. Axonal outgrowth was examined by measuring the transport of 35[S]methionine-labeled structural proteins (tubulin, actin, and neurofilament triplet) from "parent" axon stumps into "daughter" axon sprouts. Since these proteins are conveyed by the slow component of axonal transport at 1-5 mm/d (1.2-6.0 X 10(-8) m/s), the isotope was injected into the spinal cord 1 wk before the testing lesion. Nerves were removed 8 d after the testing lesion, sectioned into 3-mm segments, and homogenized; soluble proteins were separated by polyacrylamide gel electrophoresis. Fluorographs were used as templates to identify gel segments for removal, solubilization, and liquid scintillation counting. Distributions of mean radioactivity for tubulin, actin, and neurofilament triplet were plotted for animals receiving a conditioning vs sham-conditioning lesion. Greater amounts of tubulin and actin were transported into daughter axons in the conditioned group. Tubulin was mainly increased in axon shafts, whereas actin was mainly increased in axon tips. These findings suggest that the axonal transport of tubulin and actin governs the rate of elongation.
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001370:Axonal Transport; D001369:Axons / Q000378:metabolism* / Q000502:physiology; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D007381:Intermediate Filament Proteins / Q000378:metabolism; D008297:Male; D008715:Methionine; D009046:Motor Neurons / Q000378:metabolism / Q000502:physiology; D009409:Nerve Crush; D009416:Nerve Regeneration; D016900:Neurofilament Proteins; D051381:Rats; D011919:Rats, Inbred Strains; D012584:Sciatic Nerve / Q000378:metabolism* / Q000502:physiology; D013997:Time Factors; D014404:Tubulin / Q000378:metabolism
chemical_list: D000199:Actins; D003598:Cytoskeletal Proteins; D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins; D014404:Tubulin; D008715:Methionine