Purification of Escherichia coli DNA helicase I from plasmid-transformed cells.
DNA helicase I was purified in large quantity from Escherichia coli cells harboring a plasmid that carries the gene encoding helicase I--the traI gene of the F sex factor--cloned in a high copy number vector. Electron microscopic studies on the purified material reveal new properties of the enzyme protein.
D004265:DNA Helicases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004262:DNA Restriction Enzymes; D004277:DNA, Single-Stranded / Q000378:metabolism; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D029968:Escherichia coli Proteins; D005144:F Factor; D005798:Genes, Bacterial; D007158:Immunologic Techniques; D008854:Microscopy, Electron; D010957:Plasmids; D014169:Transformation, Bacterial
D004277:DNA, Single-Stranded; D029968:Escherichia coli Proteins; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; C024400:GTYRAC-specific type II deoxyribonucleases; C027093:TraI protein, E coli; D004265:DNA Helicases