Combined GABA-immunocytochemistry and TMB-HRP histochemistry of pretectal nuclei projecting to the inferior olive in rats, cats and monkeys.
Cells in the pretectal nucleus of the optic tract (NOT) of rats, cats and monkeys were retrogradely labeled with horseradish peroxidase (HRP) stereotaxically injected into the inferior olive (IO). A procedure for stabilizing the tetramethylbenzidine (TMB)-HRP reaction product was used to visualize combined TMB-HRP and immunohistochemically localized gamma-aminobutyric acid (GABA) in the same sections. Positive GABAergic reaction product was found to be restricted to smaller-size intrinsic neurons. Larger NOT cells projecting to the IO were consistently free of GABA reaction product and, in addition, appeared to be contacted by relatively few GABAergic terminals.
D000818:Animals; D002415:Cats; D008253:Macaca mulatta; D008636:Mesencephalon / Q000378:metabolism*; D009434:Neural Pathways / Q000378:metabolism; D009847:Olivary Nucleus / Q000378:metabolism*; D051381:Rats; D013194:Staining and Labeling; D014795:Visual Pathways / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D005680:gamma-Aminobutyric Acid