title: Co-release of acetylcholine, glutamate and taurine from synaptosomes of Torpedo electric organ.
abstract: The amino acid content of Torpedo synaptosomes was investigated. It was found that glutamate and taurine were particularly enriched, their concentration exceeding that of acetylcholine by 50-60%. Other amino acids such as alanine, gamma-aminobutyric acid (GABA), glycine, leucine and phenylalanine were also present, but in smaller quantities. The neurotransmitter-releasing agents gramicidin, calcium ionophore A23187, High K+ and Glycera venom all released acetylcholine, as shown previously. However, both glutamate and taurine were found to be released by these agents together with acetylcholine. The efflux of all 3 compounds was found to be largely calcium dependent.
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology / Q000502:physiology; D004557:Electric Organ / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism*; D018698:Glutamic Acid; D006096:Gramicidin / Q000494:pharmacology; D066298:In Vitro Techniques; D013574:Synaptosomes / Q000378:metabolism*; D013654:Taurine / Q000378:metabolism*; D014101:Torpedo
chemical_list: D000596:Amino Acids; D005971:Glutamates; D006096:Gramicidin; D013654:Taurine; D000001:Calcimycin; D018698:Glutamic Acid; D000109:Acetylcholine; D002118:Calcium