Rapid purification of DNA from agarose gels by centrifugation through a disposable plastic column.
A simple and rapid method for purifying DNA from agarose gels is described. Agarose slices containing DNA are placed in a disposable plastic column and the DNA is separated from the agarose by centrifugation in a microfuge. Recoveries averaging 25% are obtained for DNA of 14 kb or less. The recovered DNA can be labeled to high specific activity, cleaved with restriction endonucleases, and ligated efficiently using standard cloning vectors.
D002498:Centrifugation / Q000379:methods; D004247:DNA / Q000302:isolation & purification*; D004262:DNA Restriction Enzymes; D010761:Phosphorus Radioisotopes; D012313:RNA / Q000302:isolation & purification; D012685:Sepharose
D010761:Phosphorus Radioisotopes; D012313:RNA; D004247:DNA; D012685:Sepharose; D004262:DNA Restriction Enzymes