Expression of the macrophage specific colony-stimulating factor (CSF-1) during human monocytic differentiation.
We and others have previously demonstrated expression of the co-fms proto-oncogene during human monocytic differentiation. The c-fms gene has since been shown to encode for the macrophage specific colony stimulating factor (CSF-1) receptor. The present results demonstrate that both CSF-1 and c-fms transcripts are induced during monocytic differentiation of human HL-60 leukemia cells. The results further demonstrate that normal human monocytes express CSF-1 RNA and that the level of these transcripts increases upon treatment with phorbol ester. Finally, the detection of CSF-1 RNA in HL-60 cells and in monocytes is associated with production of the CSF-1 gene product. These findings would suggest that monocytes are capable of regulating their own survival, growth and differentiation through CSF-1 production.
D002454:Cell Differentiation; D002460:Cell Line; D003115:Colony-Stimulating Factors / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D007951:Leukemia, Myeloid / Q000473:pathology; D008264:Macrophages; D009000:Monocytes / Q000166:cytology*; D009857:Oncogenes; D000090063:Proto-Oncogene Mas; D012313:RNA / Q000096:biosynthesis; D013755:Tetradecanoylphorbol Acetate; D014158:Transcription, Genetic
D003115:Colony-Stimulating Factors; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D012313:RNA; D013755:Tetradecanoylphorbol Acetate