A modification of the tannic acid-phosphomolybdic acid-dye stain for demonstrating myoepithelial cells in formalin fixed tissue.
A modified tannic acid-phosphomolybdic acid-dye procedure is used for staining myoepithelial cells in formalin fixed surgical and autopsy material. Paraffin sections are brought to water, mordanted for 1 hr in Bouin's fixative previously heated to 56 C, cooled while still in Bouin's, rinsed in tap water until sections are colorless, rinsed in distilled water, treated with 5% aqueous tannic acid 5-20 min, rinsed in distilled water 30 sec or less, treated with 1% aqueous phosphomolybdic acid 10-15 min, rinsed 30 sec in distilled water, rinsed in methanol, stained 1 hr in a saturated solution of amido black or phloxine B in 9:1 methanol:acetic acid, rinsed in 9:1 methanol:acetic acid, dehydrated, cleared and mounted. Myoepithelial cells of sweat, lacrimal, salivary, bronchial, and mammary glands are blue-green with amido black or pink with phloxine B. Fine processes of myoepithelial cells are well delineated. Background staining is minimal and the procedure is highly reproducible.
D000818:Animals; D001344:Autopsy; D001980:Bronchi / Q000166:cytology; D004847:Epithelial Cells; D005404:Fixatives; D005557:Formaldehyde; D006801:Humans; D047348:Hydrolyzable Tannins; D007765:Lacrimal Apparatus / Q000166:cytology; D008321:Mammary Glands, Animal / Q000166:cytology; D008982:Molybdenum; D010756:Phosphoric Acids; D012469:Salivary Glands / Q000166:cytology; D013194:Staining and Labeling; D013545:Sweat Glands / Q000166:cytology
D005404:Fixatives; D047348:Hydrolyzable Tannins; D010756:Phosphoric Acids; D005557:Formaldehyde; D008982:Molybdenum; C003125:phosphomolybdic acid