A new strain-specific cross-reactive idiotope with possible regulatory function expressed on BALB/c anti-alpha (1-3) dextran antibodies.
We obtained a mAb, B5, defining a major crossreactive Id, the B5 Id, having an in vivo regulatory function. BALB/c mice immunized against the bacterial dextran Ag B1355S produce lambda 1-bearing anti-alpha (1-3) glucosidic linkage of dextran B1355S (DEX) antibodies. Of these antibodies 30% expressed the B5 Id determinant. As was observed for the IdX-Id, B5 idiotope expression was also linked to the BALB/c H chain allotype. The antibody family expressing the B5 Id included IdX+ as well as IdX- defined monoclonal anti-alpha (1-3) DEX antibodies. Injection into BALB/c mice of mAb B5 conjugated to keyhole limpet hemocyanin led to enhancement of serum anti-alpha (1-3) DEX antibody synthesis, and to a strong increase in B5+ Ig lacking dextran-binding activity. IdX+ serum Ig were also increased in these mice. Injection of this mAb into allotype congenic nonresponder C.B20 mice induced a significant serum lambda 1-bearing anti-alpha (1-3) dextran antibody response (5 to 50 micrograms/ml). However, in A/J nonresponder mice, a similar treatment induced only the dextran-nonbinding component, which reached high levels (approximately 7 mg/ml) in the sera of these mice. In the majority of these A/J mice, we detected a specific anti-(4-hydroxy-5-iodo-3-nitrophenyl)acetyl antibody response characterized by B5 Id-positive antibodies. This observation can be explained by an in vivo regulatory idiotope-mediated cross-regulation.
D000276:Adjuvants, Immunologic / Q000032:analysis / Q000096:biosynthesis / Q000502:physiology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D003429:Cross Reactions; D003911:Dextrans / Q000276:immunology*; D007130:Immunoglobulin Idiotypes / Q000032:analysis / Q000096:biosynthesis / Q000502:physiology*; D007525:Isoelectric Focusing; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C / Q000276:immunology*; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009592:Nitrohydroxyiodophenylacetate / Q000276:immunology; D013045:Species Specificity
D000276:Adjuvants, Immunologic; D000911:Antibodies, Monoclonal; D003911:Dextrans; D007130:Immunoglobulin Idiotypes; C040834:alpha-1,3-dextran; D009592:Nitrohydroxyiodophenylacetate