[Antigenic polysaccharides of bacteria of the genus Shigella. Determination of the structure of polysaccharide chains of Shigella boydii type 9 lipopolysaccharide and detection of unusually high molecular weight glycolipid].
Specific acidic polysaccharide has been isolated from the Shigella boydii type 9 antigenic lipopolysaccharide after mild hydrolysis followed by chromatography on Sephadex G-50. The polysaccharide consists of D-glucose, D-glucuronic acid, 2-acetamido-2-deoxy-D-glucose, and L-rhamnose. From the results of methylation analysis, partial acid hydrolysis and 13C NMR data the structure of the repeating unit of the polysaccharide was deduced as follows: [----4)DGlcp(alpha 1----4)DGlcAp(beta 1----3)DGlcNAcp(alpha 1----3)LRhap(alpha 1----]n. The lipopolysaccharide from Sh. boydii 9 was fractionated by gel chromatography on the Sephadex G-200 column in a buffer containing sodium deoxycholate into three fractions. PAGE-SDS of the fractions obtained, 13C NMR- and chromato-mass-spectrometry data indicated that the three fractions contained the O-specific polysaccharide as the only carbohydrate component. The substance from the most high-molecular weight fraction contained unusually long O-specific chains (60,000 dalton). In the fat acid composition this fraction differed from other lipopolysaccharides by absence of beta-hydroxymyristic acid.
D000942:Antigens, Bacterial / Q000032:analysis; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D000939:Epitopes / Q000032:analysis*; D006017:Glycolipids / Q000032:analysis*; D008070:Lipopolysaccharides / Q000032:analysis / Q000276:immunology*; D008970:Molecular Weight; D019081:O Antigens; D012760:Shigella / Q000276:immunology*; D012761:Shigella boydii / Q000276:immunology*
D000942:Antigens, Bacterial; D000939:Epitopes; D006017:Glycolipids; D008070:Lipopolysaccharides; D019081:O Antigens
Antigennye polisakharidy bakteriĭ roda Shigella. Ustanovlenie struktury polisakharidnoĭ tsepi lipopolisakharida Shigella boydii, tip 9, i obnaruzhenie neobychnogo vysokomolekuliarnogo glikolipida.