Respiratory syncytial virus glycoprotein expression in human and simian cell lines.
Glycoproteins synthesized in both human (HeLa and HEp-2) and simian (Vero and BS-C-1) cell lines following infection with two different strains of respiratory syncytial virus (A2 and Long) were analysed by SDS-PAGE following immunoprecipitation with monoclonal antibodies. Minor virus strain-dependent differences in the large glycoprotein, G, and the fusion protein polypeptides F1 and F2 were observed together with minor cell line-dependent differences in the size of the F2 polypeptide. Major quantities of two glycoproteins, termed Ga (50K) and Gb (45K), were detected in A2 strain-, and to a lesser extent in Long strain-, infected simian cells. These proteins were also present in infected human cells, but in much reduced amounts. Immunoprecipitation with anti-G monoclonal antibodies demonstrated that Ga and Gb shared different epitopes with G.
D000818:Animals; D000956:Antigens, Viral; D002460:Cell Line; D002522:Chlorocebus aethiops; D000939:Epitopes; D006023:Glycoproteins / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D006801:Humans; D008562:Membrane Glycoproteins; D012136:Respiratory Syncytial Viruses / Q000276:immunology / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D014760:Viral Fusion Proteins; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology
D000956:Antigens, Viral; D000939:Epitopes; C028391:G protein, vesicular stomatitis virus; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; D014764:Viral Proteins