Angiogenic activity of malignant oral epithelial cells: a preliminary study.
We investigated the angiogenetic capacity of normal and malignant oral epithelial cells by intradermal injection of cells into nude mice. Cells investigated: 1) a tumorigenic cell line from rat palate; 2) a non-tumorigenic epithelial cell line from rat palate; 3) cells from second passage of rat palate epithelium explant cultures; 4) cells from epidermis of neonatal mice. A 0.1 ml suspension containing 10(5) epithelial cells was injected into the flank region. Mice were killed 8 days later to evaluate angiogenesis. Under a dissection microscope, the number of vessels in the skin adjacent to the injection site was counted. The tumorigenic cell line demonstrated the strongest angiogenetic capacity and the non-tumorigenic cell line was more angiogenic than any of the other non-tumorigenic cell types. The greater angiogenic activity of neoplastic oral epithelial cells may be a useful feature in the evaluation of oral malignant development.
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002460:Cell Line; D004848:Epithelium; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009061:Mouth Mucosa; D009062:Mouth Neoplasms / Q000473:pathology*; D009389:Neovascularization, Pathologic; D011230:Precancerous Conditions / Q000473:pathology*; D012867:Skin / Q000098:blood supply*