Protein C functional assay using snake venom activator.
We report a functional assay of Protein C on whole plasma using a snake venom called Protac-C. This method is simple, avoids absorption-elution techniques. Also this method can be adopted to a microtitre plate system testing of large number of samples. Functional levels by this test correlated well with the antigenic levels (r = .8) measured by ELISA. Protein C functional and antigenic values in 58 healthy volunteers were 82% and 95.5% respectively. The warfarinized samples showed a lower mean.
D002863:Chromogenic Compounds; D004789:Enzyme Activation / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D008722:Methods; D010455:Peptides / Q000494:pharmacology*; D011486:Protein C / Q000032:analysis* / Q000276:immunology / Q000502:physiology; D013997:Time Factors
D002863:Chromogenic Compounds; D000939:Epitopes; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D011486:Protein C; C049647:snake venom protein C activator