Flow cytometric analysis of the recruitment of G0 cells in human epidermis in vivo following tape stripping.
Tape stripping of human skin elicits a proliferative response of a synchronously-dividing group of cells. The progress of this cohort of cells has been monitored using two windows in the cell cycle, one located in mid-S phase and the other centred around G2 + M. The cellular DNA is measured with flow cytometry, the windows are defined by two ranges in the DNA histogram. The cohort can be described as the recruitment of cells from a pre-existing G0 compartment which consists of 76% of all proliferative cells. The duration of the S phase is calculated to be 10.2 hr and G2 + M phase 5.1 hr. The cell cycle time of 39 hr for normal human keratinocytes derived from these figures is in line with recent values obtained by different techniques.
D004247:DNA / Q000032:analysis; D000078404:Epidermal Cells; D004817:Epidermis / Q000032:analysis; D005434:Flow Cytometry; D006801:Humans; D007399:Interphase; D007633:Keratins; D007700:Kinetics; D008938:Mitosis
D007633:Keratins; D004247:DNA