The use of N,N,N',N'-tetramethylphenylenediamine to detect peroxidase activity on polyacrylamide electrophoresis gels.
N,N,N',N'-Tetramethylphenylenediamine (TMPD) acts as an effective indicator of peroxidase activity on polyacrylamide electrophoresis gels. The test is easy to perform, rapid, sensitive, and reliable. The procedure produces vivid bright blue bands (Wursters blue) on a clear background. TMPD and Wursters blue did not interfere with a number of other electrophoresis stains subsequently applied. These included total protein staining with Coomassie blue, and a number of pigment producing electrophoresis stains used to investigate melanogenesis-related enzymes in the black yeast Phaeococcomyces sp.
D002863:Chromogenic Compounds; D004589:Electrophoresis, Disc; D010544:Peroxidases / Q000032:analysis*; D010655:Phenylenediamines; D013194:Staining and Labeling; D013772:Tetramethylphenylenediamine; D015003:Yeasts / Q000201:enzymology
D002863:Chromogenic Compounds; D010655:Phenylenediamines; D010544:Peroxidases; D013772:Tetramethylphenylenediamine