Plasminogen activator production from murine mastocytoma cells. Dissociation of plasminogen activator and histamine production by sodium butyrate.
Plasminogen activator (PA) production from mouse mastocytoma P815 was investigated. A trace level of PA was detected in the culture supernatant of P815, but addition of Con A (5 micrograms/ml) stimulated the PA production to the 20- to 50-fold of that of the unstimulated culture. Time course studies suggest that PA is actively synthesized and secreted during the culture period with Con A. Furthermore, pretreatment of P815 with sodium butyrate, which is known to differentiate interleukin-3-dependent bone marrow mast cells or P815 cells, increased the PA production from P815 cells in the presence or absence of Con A.
D000818:Animals; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology; D006084:Graft Rejection / Q000187:drug effects; D006636:Histamine Release; D012515:Mast-Cell Sarcoma / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D010960:Plasminogen Activators / Q000096:biosynthesis*
D002087:Butyrates; D020148:Butyric Acid; D003208:Concanavalin A; D010960:Plasminogen Activators