[Validation and development of an immunofluorescence method for isolating and identifying mycobacteria L forms].
The complement fixation test and the immunofluorescence test have demonstrated that the L-forms of mycobacteria retain their species-specific and genus-specific determinants and possess serological activity. The L-variants obtained by different methods differ in size, depending on the degree of the destruction of their cell wall. Specific antisera to the L-forms of mycobacteria, suitable for use in the indirect immunofluorescence test, have been obtained. These antisera are highly specific and permit not only the rapid detection, but also identification of the L-forms.
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D003168:Complement Fixation Tests; D000939:Epitopes / Q000032:analysis; D005455:Fluorescent Antibody Technique; D007740:L Forms / Q000276:immunology / Q000302:isolation & purification*; D051379:Mice; D009161:Mycobacterium / Q000276:immunology / Q000302:isolation & purification*; D009162:Mycobacterium avium / Q000276:immunology / Q000302:isolation & purification; D009163:Mycobacterium bovis / Q000276:immunology / Q000302:isolation & purification; D009170:Nontuberculous Mycobacteria / Q000276:immunology / Q000302:isolation & purification; D011817:Rabbits; D013045:Species Specificity; D013104:Spheroplasts / Q000276:immunology / Q000302:isolation & purification
D000942:Antigens, Bacterial; D000939:Epitopes
Obosnovanie i razrabotka vyiavleniia i identifikatsii L-form mikobakteriĭ metodom immunofliuorestsentsii.