Inhibition of prolactin secretion by antiserum to the alpha- and beta-subunits of gonadotropin.
This study was undertaken to determine whether antiserum to the alpha- and beta-subunits of human gonadotropin could affect prolactin secretion from human pituitary cell monolayers. The addition of antiserum to the alpha-subunit of follicle-stimulating hormone, but not to the alpha-subunit of luteinizing hormone, at a 1:10,000 dilution inhibited basal prolactin secretion. The addition of these antisera failed to inhibit prolactin secretion in the face of 10(-5) mol/L gonadotropin-releasing hormone. Coincubation of antiserum to the beta-subunit of luteinizing hormone or the beta-subunit of follicle-stimulating hormone at a 1:10,000 dilution failed to inhibit basal prolactin secretion; however, coincubation of gonadotropin-releasing hormone with antiserum to the beta-subunit of either luteinizing hormone or follicle-stimulating hormone significantly inhibited the release of prolactin. These observations led us to conclude that basal and gonadotropin-releasing hormone-mediated prolactin secretion can be affected by gonadotropin subunits.
D000328:Adult; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000276:immunology*; D018997:Chorionic Gonadotropin, beta Subunit, Human; D004305:Dose-Response Relationship, Drug; D015292:Glycoprotein Hormones, alpha Subunit; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology*; D008297:Male; D010446:Peptide Fragments / Q000276:immunology*; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism; D010906:Pituitary Hormone-Releasing Hormones / Q000494:pharmacology; D010908:Pituitary Hormones, Anterior / Q000276:immunology*; D011388:Prolactin / Q000037:antagonists & inhibitors* / Q000378:metabolism
D006063:Chorionic Gonadotropin; D018997:Chorionic Gonadotropin, beta Subunit, Human; D015292:Glycoprotein Hormones, alpha Subunit; D007106:Immune Sera; D010446:Peptide Fragments; D010906:Pituitary Hormone-Releasing Hormones; D010908:Pituitary Hormones, Anterior; D011388:Prolactin