Does the interchromatin compartment contain actin?
Transient blockage of transcription in chick liver by alpha-amanitin results in a temporary clearing of nucleoplasmic regions in affected nuclei and concurrent exposure in situ of a discrete interchromatin framework. This is accompanied by sharp decreases in total nuclear RNA (approximately 28%) and nonhistone protein content (approximately 18%). Electrophoretic analysis of matrix fraction proteins from such highly denuded nuclei shows a marked relative enrichment in actin and RNP-derived polypeptides. These observations suggest that actin is a real constituent of the interchromatin region and supports various reports that this protein may have a basic structural role in nuclei.
D000199:Actins / Q000032:analysis*; D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D002645:Chickens; D002843:Chromatin / Q000032:analysis*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D004586:Electrophoresis; D008099:Liver / Q000648:ultrastructure*; D008854:Microscopy, Electron; D012313:RNA / Q000378:metabolism; D012261:Ribonucleoproteins / Q000032:analysis; D014158:Transcription, Genetic / Q000187:drug effects
D000199:Actins; D000546:Amanitins; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D012261:Ribonucleoproteins; D012313:RNA