Detection of a few picograms of DNA on polyacrylamide gels by silver staining.
DNA fragments separated on polyacrylamide gels are silver stained in ethanolamine solution. The staining procedure can be completed in 3 1/2 h. Illumination of the gels on a black background increases the sensitivity of detection compared with the usual transillumination. The limit level of detection is 3-5 pg per band with a cross-sectional area of 5 mm2. Five to fifty picograms of DNA may be detected quantitatively by scanning the gels. The method will detect 0.1 to 1 ng per band of low-molecular-weight RNA components.
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis; D004247:DNA / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D008832:Microchemistry; D010957:Plasmids; D012313:RNA / Q000032:analysis; D012834:Silver; D013194:Staining and Labeling
D004279:DNA, Viral; D012834:Silver; D012313:RNA; D004247:DNA