Interference of fetal hemoglobin with the spectrophotometric measurement of carboxyhemoglobin.
We measured the concentration of carboxyhemoglobin (HbCO) in blood samples from 32 neonates by spectrophotometry (IL282 CO-Oximeter) and gas chromatography, finding a strong positive correlation (r = 0.89) between the concentration of fetal hemoglobin (Hb F) and HbCO as measured by spectrophotometry, but not by gas chromatography. Thus, Hb F interferes with the determination of HbCO by spectrophotometric techniques by falsely increasing apparent HbCO in direct proportion to Hb F. We conclude that, when Hb F is known or suspected to be present, blood HbCO cannot be reliably determined by methods based on spectrophotometry.
D002263:Carboxyhemoglobin / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods; D005189:False Positive Reactions; D005260:Female; D005319:Fetal Hemoglobin; D006461:Hemolysis; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D010092:Oximetry / Q000379:methods
D005319:Fetal Hemoglobin; D002263:Carboxyhemoglobin