Studies on immunity in hybridoma-bearing mice. A. Immune response to antigens. II. inhibition of production of anti-dinitrophenyl antibodies in mice which have rejected the B 53 anti-dinitrophenyl-producing IgE hybridoma.
When BALB/c mice, which had rejected the anti-dinitrophenyl (DNP) IgE-producing hybridoma B 53, were immunized with DNP proteins, they produced much less anti-DNP antibodies than control (normal) mice. The anti-DNP plaque-forming cell (PFC) number was much less when spleen cells from mice immunized with DNP proteins were treated with sera of mice which had rejected the hybridoma B 53 than the PFC number from the same spleen cells not treated by the sera. The sera of mice which had rejected the hybridoma B 53 contained an inhibitor which was adsorbed and eluted from an anti-mouse immunoglobulin column and also a mouse anti-DNP IgG2a column. The inhibition of PFC was hapten-reversible. In Western blotting the eluates from the anti-DNP IgG2a column reacted as well with the blotted anti-DNP IgE B 53 as an anti-idiotypic antibody to anti-DNP IgE B 53. These criteria establish that the inhibitor in the sera of the mice which had rejected the B 53 tumor was an anti-idiotypic antibody of the type which mimics the epitope (DNP) of the immunizing antigen.
D000818:Animals; D000906:Antibodies / Q000276:immunology* / Q000302:isolation & purification; D000917:Antibody Formation; D000941:Antigens / Q000008:administration & dosage* / Q000276:immunology; D001323:Autoantibodies / Q000096:biosynthesis / Q000276:immunology; D003101:Collodion; D004136:Dinitrobenzenes / Q000008:administration & dosage / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D005260:Female; D006084:Graft Rejection; D006241:Haptens / Q000276:immunology; D006462:Hemolytic Plaque Technique; D006825:Hybridomas / Q000145:classification / Q000276:immunology / Q000378:metabolism / Q000637:transplantation*; D007073:Immunoglobulin E / Q000378:metabolism*; D007130:Immunoglobulin Idiotypes / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D009578:Nitrobenzenes / Q000276:immunology*
D000906:Antibodies; D000941:Antigens; D001323:Autoantibodies; D004136:Dinitrobenzenes; D000939:Epitopes; D006241:Haptens; D007130:Immunoglobulin Idiotypes; D009578:Nitrobenzenes; D007073:Immunoglobulin E; D003101:Collodion