Isolation of a cDNA copy of an RNA species expressed in murine pre-B cells.
A part of a gene, pZ 183, was isolated from a cDNA library constructed from poly(A)-containing RNA of the chemically induced murine pre-B lymphoma 70Z/3. This DNA sequence was found to be present in one of 5,000 cDNA clones in the unsubtracted library, and in one of 350 cDNA clones in a library in which sequences common to 70Z/3 and to a T cell hybridoma had been subtracted. This indicates that the corresponding gene belongs to the genes which are expressed with low to medium abundance in the pre-B cell line 70Z/3. The gene was found to be expressed in pre-B lymphomas, but not in B lymphomas, myelomas, T cell lymphomas, macrophage tumors or a fibroblast line. By in situ hybridization it was detected in 2-4% of fetal liver cells between day 15 and 16 of gestation, and in 1% of adult bone marrow cells. It was not detected in thymus cells, nor in mature T cells, spleen, adult liver, kidney, heart, lung or brain. The size of the transcript found in all pre-B cell lymphomas is 1.2 kb in length. The pZ 183 DNA sequence would appear to be a useful B-lineage specific marker which identifies pre-B cells at various stages before the mature B cell.
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology*; D001483:Base Sequence; D001853:Bone Marrow / Q000196:embryology; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002460:Cell Line; D004247:DNA / Q000302:isolation & purification*; D005333:Fetus; D006412:Hematopoietic Stem Cells / Q000166:cytology; D008099:Liver / Q000166:cytology / Q000196:embryology; D051379:Mice; D008815:Mice, Inbred Strains; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000235:genetics*; D013154:Spleen / Q000166:cytology / Q000196:embryology; D013950:Thymus Gland / Q000166:cytology / Q000196:embryology
D012313:RNA; D004247:DNA