Changing the identity of a tRNA by introducing a G-U wobble pair near the 3' acceptor end.
Although the genetic code for protein was established in the 1960's, the basis for amino acid identity of transfer RNA (tRNA) has remained unknown. To investigate the identity of a tRNA, the nucleotides at three computer-identified positions in tRNAPhe (phenylalanine tRNA) were replaced with the corresponding nucleotides from tRNAAla (alanine tRNA). The identity of the resulting tRNA, when examined as an amber suppressor in Escherichia coli, was that of tRNAAla.
D000409:Alanine / Q000235:genetics; D000596:Amino Acids / Q000235:genetics*; D001482:Base Composition; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics*; D006151:Guanosine; D009154:Mutation; D010649:Phenylalanine / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics*; D012343:RNA, Transfer / Q000235:genetics*; D012344:RNA, Transfer, Ala / Q000235:genetics; D012353:RNA, Transfer, Gly / Q000235:genetics; D012357:RNA, Transfer, Lys / Q000235:genetics; D012360:RNA, Transfer, Phe / Q000235:genetics; D013489:Suppression, Genetic; D014529:Uridine
D000596:Amino Acids; D012329:RNA, Bacterial; D012344:RNA, Transfer, Ala; D012353:RNA, Transfer, Gly; D012357:RNA, Transfer, Lys; D012360:RNA, Transfer, Phe; D006151:Guanosine; D010649:Phenylalanine; D012343:RNA, Transfer; D000409:Alanine; D014529:Uridine