title: Alphavirus replication in cultured cells and infected animals is inhibited by antiproteinase agents.
abstract: The influence of different antiproteinase agents on alphavirus replication was examined. Sindbis virus multicycle replication in cultured cells was suppressed by N-tosyl-phenylalanine chloromethyl ketone (TPCK), an inhibitor of chymotrypsin-like proteinases, and by aprotinin, an inhibitor of a wide spectrum of proteinases. Antiviral activity of TPCK was also demonstrated in Sindbis virus-infected animals. Parenteral injections of TPCK in infected mice reduced virus titers in brain and blood. The possible mechanism(s) of antiviral action of the antiproteinase agents are discussed.
mesh_terms: D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology*; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D007611:Aprotinin / Q000494:pharmacology*; D002478:Cells, Cultured; D002642:Chick Embryo; D051379:Mice; D012845:Sindbis Virus / Q000187:drug effects* / Q000502:physiology; D014036:Togaviridae Infections / Q000188:drug therapy / Q000382:microbiology; D014107:Tosyllysine Chloromethyl Ketone / Q000494:pharmacology; D014108:Tosylphenylalanyl Chloromethyl Ketone / Q000494:pharmacology*; D010948:Viral Plaque Assay; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000590:Amino Acid Chloromethyl Ketones; D000998:Antiviral Agents; D014107:Tosyllysine Chloromethyl Ketone; D014108:Tosylphenylalanyl Chloromethyl Ketone; D007611:Aprotinin