Functional expression in the Xenopus oocyte of messenger ribonucleic acids encoding brain neurotransmitter receptors: further characterisation of the implanted GABA receptor.
The Xenopus oocyte is the only system currently available for the full expression of mRNAS encoding membrane receptors and ion channels. Its application in the case of brain mRNAs for the GABAA receptor is illustrated, including the production of receptor subunits and its chloride channel with characteristic pharmacology.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D001643:Bridged Bicyclo Compounds / Q000494:pharmacology; D019086:Bridged Bicyclo Compounds, Heterocyclic; D002642:Chick Embryo; D002712:Chlorides / Q000378:metabolism; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005445:Flunitrazepam / Q000378:metabolism; D005786:Gene Expression Regulation; D006031:Glycosylation; D007473:Ion Channels / Q000378:metabolism; D009865:Oocytes / Q000378:metabolism*; D011061:Poly A / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D011963:Receptors, GABA-A / Q000235:genetics*; D014981:Xenopus; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D001643:Bridged Bicyclo Compounds; D019086:Bridged Bicyclo Compounds, Heterocyclic; D002712:Chlorides; D007473:Ion Channels; D012333:RNA, Messenger; D011963:Receptors, GABA-A; D011061:Poly A; D005680:gamma-Aminobutyric Acid; D005445:Flunitrazepam; D012313:RNA; C037476:tert-butylbicyclophosphorothionate; D004247:DNA