title: An antiserum to the sea urchin 20 S egg dynein reacts with embryonic ciliary dynein but it does not react with the mitotic apparatus.
abstract: Unfertilized sea urchin eggs contain one or more dynein-like enzymes which may be able to serve as microtubule translocators during embryonic development. There are at least two interesting possibilities for the function of the egg dynein: the enzyme may be involved in cytoplasmic microtubule movement such as mitotic spindle anaphase motion; or the enzyme may be a stored precursor for the dynein that functions in embryonic cilia, which are expressed and highly motile at the blastula stage of development. In order to determine directly the distribution and possible function of one of the previously described egg dyneins, the latent-activity 20 S egg dynein (Asai and Wilson, 1985), an antiserum was produced which was highly reactive with the important high Mr polypeptides of 20 S dynein. This antiserum reacted in "Western" immunoblots and in dot-blotting experiments with egg dynein and with embryonic ciliary dynein, but it did not react with any component of sperm flagella. Indirect double immunofluorescence microscopy demonstrated that the anti-20 S antiserum could brightly stain embryonic cilia but it did not stain the sperm flagella from the same sea urchin species. Under the same conditions that the antiserum stained cilia, anti-20 S did not stain the mitotic apparatus but it did appear to stain the cortical region of the dividing egg. In a time-course experiment, the antigen reactive with the anti-20 S antiserum gradually accumulated in the developing early sea urchin embryo. The most significant increase in the apparent concentration of the 20 S dynein occurred just prior to embryonic ciliation and during a period when the mitotic activity of the embryo was in decline. These results lead to two conclusions. First, ciliary dynein and sperm flagellar dynein, although derived from very similar organelles and from the same species of sea urchin, are immunologically distinct. Second, the 20 S egg dynein may be a stored precursor of embryonic ciliary dynein and does not appear to be a component of the mitotic apparatus.
mesh_terms: D000251:Adenosine Triphosphatases / Q000276:immunology*; D000818:Animals; D002923:Cilia / Q000201:enzymology*; D004398:Dyneins / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005407:Flagella / Q000276:immunology; D005455:Fluorescent Antibody Technique; D007106:Immune Sera / Q000276:immunology; D007159:Immunologic Tests; D008297:Male; D010063:Ovum / Q000201:enzymology*; D012617:Sea Urchins / Q000196:embryology; D013094:Spermatozoa / Q000276:immunology; D008941:Spindle Apparatus / Q000276:immunology*
chemical_list: D000939:Epitopes; D007106:Immune Sera; D000251:Adenosine Triphosphatases; D004398:Dyneins