Nucleotide sequence of the most abundantly transcribed early gene of human cytomegalovirus strain AD169.
Cytoplasmic poly(A+)RNA was isolated from human embryo fibroblast cells during the early phase of an infection with human cytomegalovirus strain AD169. These preparations contained a single abundant transcript of 2.7 kb which was derived from each of the repeat sequences flanking the long unique region of the virus genome. The gene was unspliced and poly(A+)RNA derived from it continued to accumulate in the cytoplasm of cells during the later stages of infection. This gene and the surrounding region was sequenced. A potential open reading frame of 170 amino acids was identified close to the 5'-terminus of the gene; the 2.7 kb early transcript therefore contains a long untranslated 3'-sequence. The promoter sequences of the 2.7 kb early gene show homology with corresponding regions of the HSV-1 gD and the human hsp 70 genes.
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics* / Q000502:physiology; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D006801:Humans; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000235:genetics; D011401:Promoter Regions, Genetic; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics
D004279:DNA, Viral; D012333:RNA, Messenger; D014764:Viral Proteins; D011061:Poly A; D012313:RNA; D004262:DNA Restriction Enzymes