Combined myeloid cell synchronization and chromosome G-banding by bromodeoxyuridine.
A combined high-resolution G-banding technique and cell synchronization of bone marrow cultures by BrdU is presented and compared with direct technique, standard 24-hour culture, and synchronization both with low temperature and methotrexate. Mitotic count and quality of spreading are improved in comparison with unsynchronized techniques. Precision of banding is increased significantly compared with the four other techniques used. Our procedure, which presents the advantage of combined synchronization and banding, is simple, time-saving, and provides more information about the chromosomes. Our results suggest that the GB-FPG technique should be a method of choice for the synchronization of bone marrow cells.
D001853:Bone Marrow / Q000648:ultrastructure*; D001854:Bone Marrow Cells; D001973:Bromodeoxyuridine; D002455:Cell Division; D002871:Chromosome Banding / Q000379:methods*; D006801:Humans; D007621:Karyotyping; D008727:Methotrexate; D013194:Staining and Labeling
D001973:Bromodeoxyuridine; D008727:Methotrexate