Role of calcium in the phloretin effects on sugar transport in rat small intestine.
The effect of phloretin on D-galactose transport in rat small intestine has been investigated. Phloretin enhanced tissue sugar accumulation and reduced mucosal to serosal D-galactose fluxes. Calcium-deprived bathing solutions and verapamil significantly reduced, but did not abolish, the phloretin-effects on intestinal galactose transport. Furthermore in the presence of the anticalmodulin drugs, RMI 12330A and trifluoperazine, phloretin was without effect on D-galactose transport. These findings suggest that phloretin may reduce serosal sugar permeability via an increase in Ca2+-calmodulin complex.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000502:physiology*; D002147:Calmodulin / Q000037:antagonists & inhibitors / Q000502:physiology; D005690:Galactose / Q000378:metabolism*; D007097:Imines / Q000494:pharmacology; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism*; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism; D008297:Male; D010693:Phloretin / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D014268:Trifluoperazine / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
D002147:Calmodulin; D007097:Imines; D007473:Ion Channels; D014268:Trifluoperazine; C014925:RMI 12330A; D014700:Verapamil; D010693:Phloretin; D002118:Calcium; D005690:Galactose