[Effect of 8-Br-ATP and 8-Oxy-ATP on RNA synthesis by RNA polymerase from Escherichia coli].
The effect of 8-Br-ATP and 8-oxy-ATP on RNA synthesis on calf thymus DNA and on abortive synthesis of di- and trinucleotides on promoter AI of phage T7 delta DIII DNA in the case of an incomplete set of substrates was studied. It was shown that the ATP analogs used inhibit the RNA and di- and trinucleotide synthesis. In all cases, 8-oxy-ATP was a more effective inhibitor than 8-Br-ATP. Both analogs are incapable of being the primer and they do not replace ATP in the course of abortive initiation of pppApU synthesis.
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D004247:DNA / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D012313:RNA / Q000096:biosynthesis*; D013379:Substrate Specificity
C051606:8-bromoadenosine 5'-triphosphate; D012313:RNA; C051607:8-hydroxyadenosine 5'-triphosphate; D000255:Adenosine Triphosphate; D004247:DNA; D012321:DNA-Directed RNA Polymerases
Vliianie 8-Br-ATP i 8-Oxy-ATP na sintez RNK RNK-polimerazoĭ Escherichia coli.