Amino-acid-enzyme activities in brown and white adipose tissues and in the liver of cafeteria rats. Effects of 24 hours starving.
Moderate obesity (17% excess body weight) was induced in female rats by offering a "cafeteria" diet during 82 days. The adaptive changes in five amino-acid-metabolism enzymes were determined in liver and white- and brown adipose tissues by comparison with chow fed controls both in the fed and 24-h starved states. Plasma urea levels were lower in the obese and the changes in enzymatic activities pointed to a lower rate of amino-acid metabolism in our dietary obesity group. The levels of activity of amino-acid-metabolism enzymes in brown adipose tissue were higher than in white adipose tissue and in most cases comparable to that of liver. The importance of amino acids as a fuel source in brown adipose tissue thermogenesis cannot be ruled out.
D000659:AMP Deaminase / Q000378:metabolism; D000273:Adipose Tissue / Q000201:enzymology*; D002001:Adipose Tissue, Brown / Q000201:enzymology*; D000410:Alanine Transaminase / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D005215:Fasting; D005260:Female; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D009765:Obesity / Q000378:metabolism; D051381:Rats
D000596:Amino Acids; D005969:Glutamate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000659:AMP Deaminase; D005974:Glutamate-Ammonia Ligase