[Determination of the molecular weight of microbial polysaccharides using high performance exclusion chromatography].
An automatic method of determining the molecular weight parameters (Mw, Mn) of microbial polysaccharides such as dextran, pullulan was developed based on the use of high performance size-exclusion chromatography on the two types of columns: Zorbax PSM 60 + 300 + 1000 and SynChropack GPC 100 + 500 + 1000. The Mw and Mn values were determined for a number of domestic and foreign dextran preparations. Changes in the molecular weight of pullulan and hydroxyethylstarch resulted from acid and enzymatic hydrolysis were estimated.
D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003911:Dextrans / Q000032:analysis; D005936:Glucans / Q000032:analysis; D006868:Hydrolysis; D006895:Hydroxyethyl Starch Derivatives / Q000032:analysis; D008970:Molecular Weight; D010952:Plasma Substitutes / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000032:analysis*
D003911:Dextrans; D005936:Glucans; D006895:Hydroxyethyl Starch Derivatives; D010952:Plasma Substitutes; D011135:Polysaccharides, Bacterial; C009109:pullulan
Opredelenie molekuliarnykh mass mikrobnykh polisakharidov s pomoshch'iu vysokoéffektivnoĭ ékskliuzionnoĭ khromatografii.