[A method of calculatiing immunochemical cross-reactions between homologous proteins].
The effects of amino acid substitutions within the antigenic sites, within the residues close to these sites and within other parts of the molecule on the cross-reaction with the antisera to homologous protein were considered. The method for calculus the values of cross-reactions, based on using primary structures data, X-ray coordinate of one of the homologues and the locations of the antigenic sites is proposed. The values obtained by this method for the cross-reactions of ten myoglobins from various species with antisperm-whale myoglobin sera have a good correlation with the known experimental data. The possibility of using the method to make the location of protein antigenic sites more precise is discussed.
D000596:Amino Acids; D000818:Animals; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis*; D007120:Immunochemistry; D008433:Mathematics; D009211:Myoglobin / Q000276:immunology*; D011487:Protein Conformation; D013045:Species Specificity; D014907:Whales / Q000276:immunology
D000596:Amino Acids; D000939:Epitopes; D009211:Myoglobin
Metod rascheta immunokhimicheskogo perekresta mezhdu gomologichnymi belkami.