Phleomycin resistance as a dominant selectable marker in CHO cells.
The Tn5 and the Streptoalloteichus hindustanus (Sh) ble genes conferring resistance to bleomycin-phleomycin antibiotics have been cloned into a mammalian vector under the RSV-LTR promoter. The resulting plasmids, pUT506 and pUT507 respectively, were used to transfect CHO cells by either the calcium phosphate or the recently described polybrene-DMSO method. Phleomycin- or bleomycin-resistant clones arose with a higher frequency after transfection with pUT507, and pUT507 transfectants were more resistant to both antibiotics than pUT506 transfectants. Phleomycin resistance in pUT507 transfectants was stable and associated with integration of plasmid sequences in genomic DNA. The Sh ble gene, which confers a dominant phleomycin-resistance phenotype, should provide a useful transferable selectable marker in CHO cells as well as in other animal cell lines.
D000818:Animals; D001761:Bleomycin / Q000494:pharmacology*; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics*; D005799:Genes, Dominant; D005819:Genetic Markers; D009693:Nucleic Acid Hybridization; D010692:Phleomycins / Q000494:pharmacology*; D010957:Plasmids; D014162:Transfection
D005819:Genetic Markers; D010692:Phleomycins; D001761:Bleomycin; D004247:DNA