Morphometrical studies of erythrophagocytosis in the spleen.
The elimination of altered RBC was investigated morphometrically in the rat spleen by determination of the percentage of erythrophagosomes in macrophages. PHZ and diamide treated RBC led to a time dependent increase of the percentage of erythrophagosomes in comparison to normal spleens. The deformability of the target RBC was lowered as revealed by measurements with glass micropipettes. RBC-microvesicles and diamide treated RBC are primarily loaded with IgG and undergo a rapid elimination. PHZ-RBC and cells with a prolonged stay in the spleen are sequestrated by secondary mechanisms. Details of primary and secondary elimination are discussed.
D000818:Animals; D004912:Erythrocytes / Q000276:immunology*; D007074:Immunoglobulin G / Q000378:metabolism*; D008264:Macrophages / Q000648:ultrastructure; D010587:Phagocytosis; D051381:Rats; D013154:Spleen / Q000166:cytology*
D007074:Immunoglobulin G