Immunoquantitation of cytochrome b5 and methylcholanthrene-induced cytochromes P-450.
The enzyme-linked immunosorbent assay (ELISA) has been investigated for its ability to quantitate hydrophobic proteins like cytochromes b5 and P-450 at the subnanogram level. Issues encountered that have broad significance not only for ELISA, but for other qualitative and quantitative immunoassays as well, include the effects of detergent, the discriminatory capacity of ELISA, and the method for determining an assay's selectivity.
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis / Q000096:biosynthesis*; D003573:Cytochrome b Group / Q000032:analysis*; D015786:Cytochromes b5; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes / Q000032:analysis; D007527:Isoenzymes / Q000032:analysis / Q000096:biosynthesis*; D008748:Methylcholanthrene / Q000494:pharmacology*; D051381:Rats
D000906:Antibodies; D000911:Antibodies, Monoclonal; D003573:Cytochrome b Group; D000939:Epitopes; D007527:Isoenzymes; D008748:Methylcholanthrene; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System