A common epitope between HLA-B27, -B13 and -B37 alloantigens defined by a monoclonal antibody.
An anti-HLA-B27 monoclonal antibody produced by the hybridoma technique is described. This BD.7 reagent is a cytotoxic IgM antibody. Its reactivity was studied by lymphocytotoxicity tests, indirect immunofluorescence tests and biochemical analysis against an extensive panel of peripheral blood mononuclear cells. All HLA-B27 positive samples, either from normal subjects or from patients with Ankylosing Spondylitis, were recognized by this reagent. Moreover, a cross-reaction was observed with HLA-B13 cells, and a new unexpected reaction with all HLA-B37 cell suspensions. The interest of such a reagent is discussed.
D000911:Antibodies, Monoclonal / Q000276:immunology; D003429:Cross Reactions; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006680:HLA Antigens / Q000276:immunology*; D015235:HLA-B Antigens; D059908:HLA-B13 Antigen; D015796:HLA-B27 Antigen; D059912:HLA-B37 Antigen; D006801:Humans
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006680:HLA Antigens; D015235:HLA-B Antigens; D059908:HLA-B13 Antigen; D015796:HLA-B27 Antigen; D059912:HLA-B37 Antigen