The application of lipid-soluble stains in plastic-embedded sections.
The present study was designed to develop a routine method for direct demonstration and precise localization of lipid substances in tissue sections. A panel of lipid-rich tissues was fixed in 4% buffered formaldehyde, infiltrated, and embedded in the water-soluble plastics Technovit 7100, EFL-67, and JB-4. The use of alcohol containing fluids was avoided. Staining with the lipid-soluble dyes Sudan Black B and Oil Red O revealed excellent preservation of tissue lipids in Technovit 7100 embedded sections when compared with cryostat sections of the same tissue specimens. Lipid preservation in EFL-67 and JB-4 embedded sections was inconsistent, even when infiltration and polymerization procedures were performed at 4 degrees C. Combination of lipid-soluble dyes with the periodic acid Schiff, Jones' methenamine silver, or Gomori' reticulin method allowed for an exact localization of lipids in high-quality Technovit 7100 embedded sections. The procedure herein is easily applicable in routine histopathology practice.
D000230:Adenocarcinoma / Q000473:pathology; D000302:Adrenal Cortex / Q000166:cytology; D001161:Arteriosclerosis / Q000473:pathology; D005234:Fatty Liver / Q000473:pathology; D006652:Histological Techniques; D006801:Humans; D007668:Kidney / Q000473:pathology; D007676:Kidney Failure, Chronic / Q000473:pathology; D007680:Kidney Neoplasms / Q000473:pathology; D008055:Lipids / Q000032:analysis*; D008080:Liposarcoma / Q000473:pathology; D010969:Plastics; D013194:Staining and Labeling
D008055:Lipids; D010969:Plastics