Commercially available fluorescein-conjugated monoclonal antibody for determining the in vitro activity of antimicrobial agents against Chlamydia trachomatis.
A simple, reproducible method for determining the antibiotic susceptibility of chlamydial isolates is described. Minimum inhibitory and lethal concentrations (MICs and MLCs) were determined for tetracycline and erythromycin titrated against a recent clinical isolate of Chlamydia trachomatis in McCoy cell cultures. A fluorescent antibody stain was found to be more sensitive than giemsa staining, generally giving two-fold higher values for both MICs and MLCs.
D000911:Antibodies, Monoclonal; D002692:Chlamydia trachomatis / Q000187:drug effects* / Q000276:immunology; D004917:Erythromycin / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D008826:Microbial Sensitivity Tests / Q000379:methods*; D013194:Staining and Labeling; D013752:Tetracycline / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D004917:Erythromycin; D013752:Tetracycline