Nuclear suppression of a mitochondrial RNA splice defect: nucleotide sequence and disruption of the MRS3 gene.
A mitochondrial RNA splice defect in the first intron of the COB gene (bI1) can be suppressed by a dominant nuclear mutation SUP-101. Starting with a gene bank of yeast nuclear DNA from a SUP-101 suppressor strain cloned in the YEp13 plasmid, we have isolated a recombinant plasmid which exerts a suppressor activity similar to the SUP-101 allele. The N3(2) insert of this plasmid contains an open reading frame (ORF) of 1014 bp which is transcribed to a 12 S RNA. Deletion of the 5' end of this ORF and its upstream sequences abolishes the suppressor activity. The N3(2) insert thus carries a functional gene (called MRS3) which can suppress a mitochondrial splice defect. The chromosomal equivalent of the cloned gene has been mapped to chromosome 10. Disruption of this chromosomal gene has no phenotypic effect on wild-type cells.
D001483:Base Sequence; D002872:Chromosome Deletion; D004271:DNA, Fungal / Q000235:genetics; D005799:Genes, Dominant; D005800:Genes, Fungal; D007438:Introns; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D012313:RNA / Q000235:genetics*; D012326:RNA Splicing; D012331:RNA, Fungal / Q000235:genetics; D000077278:RNA, Mitochondrial; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013489:Suppression, Genetic
D004271:DNA, Fungal; D012331:RNA, Fungal; D000077278:RNA, Mitochondrial; D012313:RNA