[Effect of elevated temperature on the persistence of measles virus vaccinal strain L-16 in a human cell culture].
The influence of high temperature (40 degrees C) on the virus carrier state and synthesis of virus-specific macromolecules in the HEp-2 cells--measles virus system was studied. The fluorescent antibody technique showed that cultivation at this high temperature led to changes in virus antigen morphology, a decrease in the number and then complete disappearance of the cells producing virus-specific antigen. Simultaneously, gradual cessation of synthesis of all kinds of virus-specific RNA was observed in chronically infected cells. The method of radioimmunoprecipitation showed the incubation at 40 degrees C to affect first of all the synthesis of virus nucleocapsid protein. The temperature-sensitive nature of synthesis of virus macromolecules may be explained by the existence of its mutations in the genome of the persisting virus.
D000956:Antigens, Viral / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006358:Hot Temperature; D006801:Humans; D008459:Measles virus / Q000254:growth & development* / Q000276:immunology; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis; D011863:Radioimmunoassay; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis; D014776:Virus Cultivation
D000956:Antigens, Viral; D000939:Epitopes; D012367:RNA, Viral; D014764:Viral Proteins
Vliianie povyshennoĭ temperatury na persistentsiiu vaktsinnogo shtamma L-16 virusa kory v kul'ture kletok cheloveka.