title: Inactivation of TTX-sensitive and TTX-insensitive sodium channels of rat myoballs.
abstract: Myoballs, spherical muscle cell regenerates, have two types of Na+ channels, the adult type with a high sensitivity to the Na+ channel blocker tetrodotoxin (TTX), and the juvenile type with 10 times less TTX sensitivity. The potential dependence of the steady-state inactivation of the Na+ channels (h infinity-curve) was studied in 50 rat myoballs with the whole-cell patch-clamp method which provides overall results from all the Na+ channels in an investigated myoball. At 37 degrees C, the h infinity-curve had the usual shape, but below 30 degrees C it was indented in a way suggesting contributions from two populations of Na+ channels with a different potential dependence of inactivation. A more distinct separation of the two components was possible by differentiating the h infinity-curve with respect to the prepulse potential. The population requiring a more negative potential for the removal of inactivation had a low sensitivity to TTX while the other population had TTX sensitivity similar to that of Na+ channels in adult muscle. At 37 degrees C, the separation between the two h infinity-curves was small. At lower temperatures, the separation became larger. This change was almost entirely due to the h infinity-curve of the TTX-insensitive channels shifting towards more negative membrane potentials.
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D007473:Ion Channels / Q000187:drug effects*; D008564:Membrane Potentials; D009132:Muscles / Q000187:drug effects*; D051381:Rats; D012964:Sodium / Q000378:metabolism*; D013696:Temperature; D013779:Tetrodotoxin / Q000494:pharmacology*
chemical_list: D007473:Ion Channels; D013779:Tetrodotoxin; D012964:Sodium