title: Role of interferon in lymphocyte recruitment into the skin.
abstract: Large numbers of lymphocytes are recruited from the blood into sites of cutaneous DTH reactions. Our goal was to investigate the factors controlling this recruitment. 111In-labeled peritoneal exudate lymphocytes were injected iv and the accumulation of these cells in skin sites injected with a variety of stimuli, was used to measure lymphocyte recruitment in rats. Large numbers of lymphocytes migrated into vaccinia- and KLH-injected sites in sensitized animals, but only into the viral and not the KLH lesions in non-immune animals. Lymphocytes also migrated efficiently into sites injected with the alpha-interferon (IFN) inducers, uv-inactivated vaccinia virus and poly I:C, as well as into sites injected with IFN. In each case there was a dose-response relationship. Analysis of the kinetics of lymphocyte recruitment demonstrated that the peak rate of migration occurred most rapidly after the injection of IFN, later after poly I:C, and was slowest to be reached after vaccinia virus. Rabbit anti-IFN blocked the recruitment of lymphocytes by uv-inactivated vaccinia and by IFN. Histologically, all of these sites demonstrated a dense mononuclear cell infiltrate in the dermis. It is suggested that IFN may be an important mediator in the recruitment of lymphocytes into inflammatory reactions.
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D004306:Dose-Response Relationship, Immunologic; D006433:Hemocyanins / Q000276:immunology; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D007372:Interferons / Q000502:physiology*; D007700:Kinetics; D008214:Lymphocytes / Q000502:physiology*; D008297:Male; D011070:Poly I-C / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000166:cytology*; D014616:Vaccinia virus / Q000276:immunology / Q000528:radiation effects
chemical_list: D007106:Immune Sera; D007372:Interferons; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin; D011070:Poly I-C