[Alkylation of tRNAPhe with 4-(N-2-chloroethyl-N-methylamino) benzyl-5'-phosphamide of d(ATTTTCA)].
Alkylation of E. coli tRNAPhe with 4-(N-2-chloroethyl-N-methylamino)benzyl-5'-phosphamide of oligonucleotide d(ATTTTCA) complementary to the sequence UGAAms2i6AA psi in the anticodon loop of tRNAPhe was studied. Three guanine residues--G28/29, G24 and G10 were found to be alkylated. Two binding sites for the reagent in the tRNA were assumed to be present. The efficiency of the alkylation of tRNA from these sites as well as an average association constant (Ka 3,8 X 10(3)M-1) for the reagent interaction with tRNA were evaluated.
D000478:Alkylation; D000926:Anticodon; D001483:Base Sequence; D004926:Escherichia coli / Q000032:analysis / Q000235:genetics; D006868:Hydrolysis; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides / Q000032:analysis; D012329:RNA, Bacterial / Q000032:analysis / Q000235:genetics; D012346:RNA, Transfer, Amino Acyl / Q000032:analysis* / Q000235:genetics
D000926:Anticodon; D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides; D012329:RNA, Bacterial; D012346:RNA, Transfer, Amino Acyl
Alkilirovabnie tRNKPhe 4-(N-2-khlorétil-N-metilamino) benzil-5'-fosfamidom d(ATTTTCA).