Viability and recovery from degranulation of isolated rat peritoneal mast cells.
Using a culture system that allows prolonged maintenance of purified populations of peritoneal mast cells, we have examined them following stimulation by non-immunologic or immunologic agents. Employing phase-contrast microscopy of living cells and various pharmacological manipulations, we have noted that the recovery process includes a reduction in cell size, the probable sealing of exocytotic cavities, a pronounced displacement of the cell nucleus and a resynthesis of histamine. During recovery, mast cells can entrap molecules from the extracellular fluid and later release these substances by a Ca-dependent mechanism. Our results suggest that microfilaments, calmodulin, Ca, and metabolic energy are necessary for recovery.
D000818:Animals; D001769:Blood; D002467:Cell Nucleus / Q000648:ultrastructure; D002470:Cell Survival; D002478:Cells, Cultured; D003594:Cytoplasmic Granules / Q000502:physiology*; D004996:Ethidium; D005089:Exocytosis; D006636:Histamine Release; D006639:Histidine / Q000494:pharmacology; D007444:Inulin / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D012430:Ruthenium Red; D012701:Serotonin / Q000378:metabolism; D013194:Staining and Labeling; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D012430:Ruthenium Red; D012701:Serotonin; D003189:p-Methoxy-N-methylphenethylamine; D006639:Histidine; D007444:Inulin; D004996:Ethidium