A preparation for patch clamp studies of labelled, identified neurones from guinea pig spinal cord.
Details are described of techniques which allow the isolation of labelled, identified neurones suitable for patch clamp recording from the guinea pig spinal cord. Fluorescent labels, injected into either the hindlimb muscles or the cerebellum, are retrogradely transported to motoneurones or dorsal spinocerebellar tract neurones respectively. Cells are then enzymatically dissociated from spinal cord slices and identified using fluorescence microscopy. Patch clamp or whole cell recordings are then made.
D000344:Afferent Pathways / Q000502:physiology; D000578:Amidines; D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002531:Cerebellum / Q000502:physiology; D004594:Electrophysiology / Q000379:methods*; D006168:Guinea Pigs; D007473:Ion Channels / Q000502:physiology*; D009046:Motor Neurons / Q000502:physiology; D009482:Neurophysiology / Q000379:methods*; D012235:Rhodamines; D013116:Spinal Cord / Q000166:cytology / Q000502:physiology*; D013194:Staining and Labeling / Q000379:methods
D000578:Amidines; D007473:Ion Channels; D012235:Rhodamines; C031455:diamidino compound 253-50