Detection of antibodies to avian reverse transcriptase by indirect ELISA.
An improved indirect ELISA test for detection of antibodies to the reverse transcriptase (revertase) is described. The sensitivity of the revertase ELISA test was compared to that of the revertase inhibition test. Serum samples of various origin and sera of specific pathogen free (SPF) hens were examined for the revertase antibodies. The presence of these antibodies in the sera of SPF chickens is discussed.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D001353:Avian Leukosis / Q000201:enzymology / Q000276:immunology; D001354:Avian Leukosis Virus / Q000201:enzymology*; D009189:Avian Myeloblastosis Virus / Q000201:enzymology* / Q000276:immunology; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D012194:RNA-Directed DNA Polymerase / Q000276:immunology*; D013047:Specific Pathogen-Free Organisms
D000914:Antibodies, Viral; D012194:RNA-Directed DNA Polymerase