Properties of monospecific antibodies to the glycoprotein of western equine encephalitis virus.
Monospecific (MSp-) antisera against E1 and E2 glycoproteins of western equine encephalitis (WEE) virus were prepared and examined for binding activities to whole virions, hemagglutination-inhibition (HI), neutralization (NT) and protection. Both anti-E1 and anti-E2 MSp-Abs protected mice against WEE virus challenge. A competition experiment with monoclonal antibodies showed that these MSp-antisera appear to lack the antibody population for some epitopes involved in viral neutralization.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000032:analysis*; D000918:Antibody Specificity; D004591:Electrophoresis, Polyacrylamide Gel; D004667:Encephalitis Virus, Western Equine / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D006023:Glycoproteins / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests; D051379:Mice; D009500:Neutralization Tests; D014771:Virion / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000939:Epitopes; D006023:Glycoproteins