title: RNA complementary to alfalfa mosaic virus RNA 4 is not translated in vitro.
abstract: cDNA of the subgenomic messenger RNA (RNA 4) of alfalfa mosaic virus was ligated in both orientations into plasmids containing the bacteriophage SP 6 promoter. Upon transcription in vitro these plasmids yielded synthetic full-length complementary RNA and messenger-sense RNA. The complementary RNA contained an open reading frame of 417 nucleotides; the messenger-sense RNA encodes the virus coat protein. No translation products from the complementary RNA were detected in a wheat germ translation system, a reticulocyte lysate translation system, or Xenopus oocytes, whereas the messenger sense RNA was very active in these translation systems.
mesh_terms: D001345:Autoradiography; D004591:Electrophoresis, Polyacrylamide Gel; D000455:Medicago sativa; D009029:Mosaic Viruses / Q000235:genetics*; D010957:Plasmids; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics*; D018075:RNA, Complementary; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*
chemical_list: D018075:RNA, Complementary; D012333:RNA, Messenger; D012367:RNA, Viral; D012313:RNA