[Cation transport and content in serum-stimulated CHO-773 cells. II. Late changes in rubidium influx and intracellular potassium content].
Serum stimulation of stationary cultures of Chinese hamster ovary cells CHO-K1 (clone 773) is accompanied by sustained increase in ouabain-sensitive rubidium (potassium) influx which results in the elevation of intracellular potassium content from 0.5-0.6 to 0.7-0.8 mmole per gram of protein. Cytofluorometric studies of serum-stimulated CHO-773 cultures have shown that the intracellular potassium increase is necessary for successful G1----S progression. The elevation of intracellular potassium was found to occur simultaneously with the cellular protein growth. Cycloheximide (10 micrograms/ml) does not influence the early Na,K-ATPase activation induced by serum; however, it abolishes the sustained increase of both rubidium influx and intracellular potassium content. In serum stimulated cells ouabain increases the potassium efflux; this ouabain effect is not observed after S phase, when rubidium (potassium) influx decreases and intracellular potassium content stops growing.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D007106:Immune Sera / Q000494:pharmacology*; D007473:Ion Channels / Q000187:drug effects* / Q000378:metabolism; D011188:Potassium / Q000378:metabolism*; D012413:Rubidium / Q000378:metabolism*; D013268:Stimulation, Chemical; D013997:Time Factors
D003470:Culture Media; D007106:Immune Sera; D007473:Ion Channels; D004247:DNA; D012413:Rubidium; D011188:Potassium
Transport i soderzhanie kationov v kletkakh CHO-773, stimulirovannykh syvorotkoĭ. II. Pozdnie izmeneniia vkhodnogo potoka rubidiia i vnutrikletochnogo soderzhaniia kaliia.