In vivo transfer of delayed hypersensitivity to Trypanosoma cruzi antigens with polysomal immune RNA.
The polysomal immune RNA (iRNA) was extracted from the spleens of mice infected with Trypanosoma cruzi. The RNA isolated from normal (nRNA) animals served as control. We found that the polysomal iRNA is able to transfer delayed-type hypersensitivity (DTH) in vivo to T. cruzi antigens as assessed by the inhibition of cell migration assay and skin test. We also demonstrated that this phenomenon is antigen specific. The polysomal iRNA preparations were fractionated by affinity chromatography on oligo (dT)-cellulose column. Our results suggest that the poly (A)-containing iRNA is the fraction responsible for transferring DTH in vivo to T. cruzi antigens.
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D002464:Cell Migration Inhibition; D014355:Chagas Disease / Q000276:immunology*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007116:Immunization, Passive; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D051379:Mice; D011061:Poly A / Q000276:immunology; D012313:RNA / Q000032:analysis / Q000276:immunology*; D012333:RNA, Messenger; D011817:Rabbits
D000953:Antigens, Protozoan; D000939:Epitopes; D012333:RNA, Messenger; C013674:RNA, immune; D011061:Poly A; D012313:RNA