Rapid lysis technique for mycobacterial species.
The lysis of mycobacterial cells typically has been difficult and time-consuming. We report a method for the physical rupture of Mycobacterium paratuberculosis and several other members of the family Mycobacteriaceae using a Mini-Beadbeater cell disrupter (Biospec Products; Bartlesville, Okla.) and zirconium beads, a process which yields DNA and RNA of high molecular weight and in greater quantity than that obtained by rupture in a Ribi pressure cell.
D000818:Animals; D001431:Bacteriological Techniques; D001433:Bacteriolysis; D004269:DNA, Bacterial / Q000302:isolation & purification*; D006801:Humans; D009161:Mycobacterium / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010283:Paratuberculosis / Q000382:microbiology; D012329:RNA, Bacterial / Q000302:isolation & purification*; D013997:Time Factors
D004269:DNA, Bacterial; D012329:RNA, Bacterial