A murine monoclonal antibody derived from the immunization of human thyroglobulin reacts equally with L-thyroxine and reverse triiodo-L-thyronine and has a unique idiotype.
Murine monoclonal antibody (mAb 16.3.2) to human thyroglobulin which bound equally to various thyroglobulins derived from nine species was obtained from the fusion of C3H/He spleen cells sensitized with normal human thyroglobulin. Characterization of mAb 16.3.2 revealed that both L-thyroxine (T4) and reverse triiodo-L-thyroxine (rT3) were very efficient in the competitive binding inhibition test and that a molar ratio between T4 and rT3 needed for 50% inhibition of binding to immunized thyroglobulin was about 1:1. Further studies on the idiotype of mAb 16.3.2 using both binding and competitive binding inhibition tests showed that mAb 16.3.2 had a unique idiotype not cross-reacting with other monoclonal antibodies to thyroglobulins. Therefore, a possible explanation offered was that mAb 16.3.2 was endowed with a unique idiotype to be regulated by a distinct idiotype network from those of other mAbs.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D001667:Binding, Competitive; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis; D006801:Humans; D007114:Immunization; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D013954:Thyroglobulin / Q000276:immunology*; D013974:Thyroxine / Q000276:immunology*; D014285:Triiodothyronine, Reverse / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D014285:Triiodothyronine, Reverse; D013954:Thyroglobulin; D013974:Thyroxine