Construction of a series of several self-cleaving RNA duplexes using synthetic 21-mers.
Two fragments (21-mers) containing consensus sequences for the self-cleavage domain in transcripts of satellite DNA of the newt were chemically synthesized and found to be cleaved in the presence of Mg2+. The cleaved product contained the 3'-terminal 2',3'-cyclic phosphate. Twenty-five combinations of partially double-stranded 21-mer RNA which contained different bases within the consensus sequences and at the cleavage sites were tested for self-cleavage. It seemed that guanosine 3'-phosphate was not susceptible to transesterification at the cleavage site.
D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D004276:DNA, Satellite / Q000235:genetics; D004951:Esterification; D008274:Magnesium / Q000494:pharmacology; D011131:Polyribonucleotides; D012313:RNA / Q000138:chemical synthesis* / Q000378:metabolism; D012330:RNA, Double-Stranded / Q000138:chemical synthesis / Q000378:metabolism; D012455:Salamandridae / Q000235:genetics; D014158:Transcription, Genetic
D004276:DNA, Satellite; D011131:Polyribonucleotides; D012330:RNA, Double-Stranded; D012313:RNA; D008274:Magnesium