Site-directed protection of RNA during nuclease digestion.
We report the use of oligodeoxynucleotides to block the nucleolytic hydrolysis of single-stranded regions of RNA. Using complementary oligomers, the hydrolysis of the CCA terminus of methionine initiator tRNA could be prevented. This method can be useful in the production of specific single-stranded fragments of RNA, which are necessary in recombinant RNA technology.
D001483:Base Sequence; D006868:Hydrolysis; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D009843:Oligoribonucleotides / Q000302:isolation & purification; D012313:RNA; D012260:Ribonucleases / Q000378:metabolism*; D013379:Substrate Specificity
D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides; D012313:RNA; D012260:Ribonucleases