[Distribution of enzymes of nucleic acid biosynthesis and their precursors during fractionation of Escherichia coli MRE-600 extract].
Distribution of the enzymes of template-dependent and template-independent polynucleotide syntheses (DNA-polymerase I, RNA-polymerase, polynucleotide phosphorylase) as well as those of the biosynthesis of nucleic acids precursors (nucleotide kinases, acetokinase and nucleoside deoxyribosyltransferases) during fractionation of Escherichia coli MRE-600 cell extract was studied. On the basis of the results obtained a technological scheme was developed that enabled to combine routine procedures of purification of the above mentioned enzymes.
D004256:DNA Polymerase I / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000096:biosynthesis*; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism; D010430:Pentosyltransferases / Q000302:isolation & purification; D011117:Polyribonucleotide Nucleotidyltransferase / Q000302:isolation & purification; D012329:RNA, Bacterial / Q000096:biosynthesis*
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D010430:Pentosyltransferases; C021888:nucleoside deoxyribosyltransferase; D012321:DNA-Directed RNA Polymerases; D004256:DNA Polymerase I; D011117:Polyribonucleotide Nucleotidyltransferase
Raspredelenie fermentov biosinteza nukleinovykh kislot i ikh predshestvennikov pri fraktsionirovanii ékstrakta Escherichia coli MRE-600.