[A rapid method for staining semithin tissue sections embedded in araldite].
A new rapid method is proposed for staining of semithin sections. The method involves the treatment of sections with a methylene blue solution with a slight heating for 1-3 minutes. The method allows to receive polychromatic contrast preparations, to save time and reagents. It also permits to avoid restaining effect. The preparations can be preserved for a long time in plastic media (e.g. in polystyrene) for light microscopy. A comparative analysis of the staining methods used in the electron microscopic practice is given.
D000818:Animals; D003584:Cytological Techniques; D004853:Epoxy Resins; D008751:Methylene Blue; D008854:Microscopy, Electron / Q000379:methods; D010796:Phthalic Anhydrides; D013194:Staining and Labeling / Q000379:methods*; D013997:Time Factors
D004853:Epoxy Resins; D010796:Phthalic Anhydrides; C005752:araldite; D008751:Methylene Blue
Uskorennyĭ metod okraski polutonkikh srezov tkaneĭ, zalitykh v araldit.