title: The peplomer protein sequence of the M41 strain of coronavirus IBV and its comparison with Beaudette strains.
abstract: The amino acid sequence of the gene for the peplomer protein of the vaccine strain M41 and the Beaudette laboratory strain M42-Salk of avian infectious bronchitis virus (IBV) have been derived from cDNA sequences. As found with other coronaviruses, the peplomer protein carries the epitopes eliciting neutralizing antibodies. The gene encodes a primary translation product of 1162 amino acids with a molecular weight of 128,079. The use of a recent algorithm to predict membrane-protein interactions led to the unambiguous localization of the signal peptide and a transmembrane anchor alpha-helix at the C-terminus. At 50 positions amino acid differences were found between M41 and two Beaudette strains (M42-Salk and M42-Houghton). They are partly clustered in two regions of the protein. These two regions are candidates for neutralization epitopes of the protein.
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003332:Coronaviridae / Q000235:genetics*; D004247:DNA / Q000032:analysis; D000939:Epitopes / Q000032:analysis; D005796:Genes; D005814:Genes, Viral; D001351:Infectious bronchitis virus / Q000235:genetics*; D011487:Protein Conformation; D013045:Species Specificity; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D000939:Epitopes; D014759:Viral Envelope Proteins; D004247:DNA