T lymphocyte-mediated protection against Pseudomonas aeruginosa infection in granulocytopenic mice.
BALB/c mice immunized with Pseudomonas aeruginosa immunotype 1 polysaccharide develop protective T cell immunity to bacterial challenge. In vitro, T cells from immunized mice kill P. aeruginosa by production of a bactericidal lymphokine. The present study demonstrates that adoptive transfer of T cells from immunized BALB/c mice to granulocytopenic mice resulted in 97% survival on challenge with P. aeruginosa, compared with 17% survival with adoptive transfer of T cells from nonimmune BALB/c mice. This protection is specifically elicited by reexposure to the original immunizing antigen; adoptive recipients cannot withstand challenge with immunotype 3 P. aeruginosa. However, the adoptive recipients do survive simultaneous infection with both P. aeruginosa immunotypes 1 and 3. Adoptive transfer of T cells from the congenic CB.20 mice, which are unable to kill P. aeruginosa in vitro, provides only 20% protection to granulocytopenic mice. These studies indicate that transfer of specific immune T lymphocytes can significantly enhance the resistance to P. aeruginosa infection in granulocytopenic mice.
D000380:Agranulocytosis / Q000276:immunology*; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology / Q000637:transplantation; D000939:Epitopes / Q000276:immunology; D005260:Female; D007113:Immunity, Innate; D007116:Immunization, Passive; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D011552:Pseudomonas Infections / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000276:immunology; D013045:Species Specificity; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology* / Q000637:transplantation
D000942:Antigens, Bacterial; D000939:Epitopes