Retrograde filling of pyramidal neurons in postmortem human cerebral cortex using horseradish peroxidase.
A method for retrograde filling of neurons in the postmortem human brain is described. Fresh human brains with minimal postmortem delay (3-11 h) were used. Horseradish peroxidase (HRP) crystals were applied directly to white matter underlying the cerebral cortex. After 5 min, the HRP was washed off and the tissue incubated in the cold for 24-48 h prior to frozen sectioning and development with 3,3'-diaminobenzidine. Reaction product filled pyramidal neurons and fibers in laminae V and VI. The method appears to be useful for labelling cortical projection neurons and cortical afferent fibers and should be applicable to other brain areas.
D000368:Aged; D000369:Aged, 80 and over; D001370:Axonal Transport; D002540:Cerebral Cortex / Q000033:anatomy & histology / Q000166:cytology* / Q000378:metabolism; D006651:Histocytochemistry / Q000379:methods*; D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D010544:Peroxidases
D006735:Horseradish Peroxidase; D010544:Peroxidases