title: Short-term rescue by RNA injection of a mitotic arrest mutation that affects the preimplantation mouse embryo.
abstract: The mutation oligosyndactyly results in syndactyly, abnormal fusion and insertion of certain limb muscles, and diabetes insipidus in heterozygous mice. When homozygous the mutation is lethal; beginning at the blastocyst stage, the homozygous cells arrest in metaphase with intact spindles. The mutant phenotype cannot be corrected by forming aggregation chimeras with wild-type cells, suggesting that the mutation results in a cell autonomous lethal condition. Short-term rescue of the homozygous-induced mitotic arrest can be achieved, however, by cytoplasmic injection of polyadenylated RNA obtained from a rapidly dividing embryo-derived stem cell line.
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology; D002678:Chimera; D004622:Embryo, Mammalian / Q000032:analysis; D006720:Homozygote; D051379:Mice; D008810:Mice, Inbred C57BL; D008845:Microinjections; D008938:Mitosis / Q000187:drug effects*; D008940:Mitotic Index; D009154:Mutation; D011061:Poly A / Q000494:pharmacology*; D012313:RNA / Q000494:pharmacology*; D012333:RNA, Messenger; D013234:Stem Cells / Q000032:analysis
chemical_list: D012333:RNA, Messenger; D011061:Poly A; D012313:RNA