Characterization of murine monoclonal antibodies that recognize neutralizing epitopes on human C5a.
We generated a panel of 10 murine monoclonal antibodies (MAbs) that recognize human complement fragment C5a. These MAbs were characterized for their ability to immunoprecipitate 125I-labeled C5a, bind C5a in solid-phase enzyme immunoassay, and block 125I-labeled C5a binding to polymorphonuclear leukocytes. Four of these MAbs had affinity constants for C5a in the 1 X 10(9) to 3 X 10(9) M-1 range. These MAbs blocked C5a-induced neutrophil polarization and chemiluminescence. They blocked the ability of passively administered C5a to cause neutropenia in rabbits. These anti-C5a neutralizing MAbs may have potential therapeutic use in states of complement activation.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D003167:Complement Activation; D003182:Complement C5 / Q000276:immunology*; D015936:Complement C5a; D000939:Epitopes; D006098:Granulocytes / Q000502:physiology; D006801:Humans; D008163:Luminescent Measurements; D009500:Neutralization Tests; D009504:Neutrophils / Q000502:physiology
D000911:Antibodies, Monoclonal; D003182:Complement C5; D000939:Epitopes; D015936:Complement C5a