[Technical parameters influencing the reactivity of the vascular endothelium in relation to lectin of Evonymus europaeus].
Some parameters characterizing sample preparation for coloration with lectins were investigated in the model of sheep myocardial capillaries, revealed by EEA after formol fixation and paraffin embedding. The most influential factors are: addition of lysine-periodate to paraformaldehyde, duration of fixative immersion, oldness of paraformaldehyde dilution, delay of fixation (if below 8 h). All of them happen before embedding. On the other hand, those happening later are out of influence.
D000818:Animals; D002196:Capillaries / Q000378:metabolism; D003331:Coronary Vessels / Q000378:metabolism*; D004727:Endothelium / Q000378:metabolism*; D006652:Histological Techniques; D037102:Lectins / Q000378:metabolism*; D012756:Sheep; D013048:Specimen Handling; D013194:Staining and Labeling; D014021:Tissue Preservation
D037102:Lectins
Paramètres techniques influençant la réactivité de l'endothélium vasculaire vis-à-vis de la lectine d'Evonymus europaeus.