Human placental cells in culture: a panning technique using a trophoblast-specific monoclonal antibody for cell separation.
A monoclonal antibody specific to human trophoblast has been used to separate these cells from a mixture of heterogeneous cell types following trypsinization of placental villi. A panning technique was used whereby antibody-labelled cells were incubated on a goat-anti-mouse-IgG-coated surface. The adhered cells were kept in culture for several days and assessed using three different monoclonal antibodies as cell markers. These cultures were shown to be enriched in trophoblast cells.
D000911:Antibodies, Monoclonal; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007633:Keratins / Q000032:analysis; D011247:Pregnancy; D013194:Staining and Labeling; D014327:Trophoblasts / Q000032:analysis / Q000166:cytology* / Q000276:immunology; D014357:Trypsin
D000911:Antibodies, Monoclonal; D007633:Keratins; D014357:Trypsin