Stimulation of cytokine production in mice using deacetylated chitin.
The effect of 70% deacetylated chitin (DAC-70) on the production of cytokines in mice was examined. DAC-70 stimulated the production of colony-stimulating factor and interferon at 6 to 12 h and 24 h after intraperitoneal injection, respectively. Interleukin 1 and colony-stimulating activity were induced in the supernatants of thioglycolate-induced macrophages stimulated with DAC-70 in vitro. However, DAC-70 did not stimulate the production by spleen cells of interleukin 2, interferon, colony-stimulating or macrophage-activating factor or of interferon by macrophages in vitro. DAC-70 showed no effect on the production of tumour necrosis factor in vivo.
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D001688:Biological Products / Q000378:metabolism*; D002686:Chitin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D003115:Colony-Stimulating Factors / Q000096:biosynthesis; D016207:Cytokines; D005260:Female; D006023:Glycoproteins / Q000096:biosynthesis; D006131:Growth Inhibitors / Q000096:biosynthesis; D007372:Interferons / Q000096:biosynthesis; D007375:Interleukin-1 / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D008222:Lymphokines / Q000096:biosynthesis; D008262:Macrophage Activation; D016215:Macrophage-Activating Factors; D051379:Mice; D008810:Mice, Inbred C57BL; D014409:Tumor Necrosis Factor-alpha
D000276:Adjuvants, Immunologic; D001688:Biological Products; D003115:Colony-Stimulating Factors; D016207:Cytokines; D006023:Glycoproteins; D006131:Growth Inhibitors; D007375:Interleukin-1; D007376:Interleukin-2; D008222:Lymphokines; D016215:Macrophage-Activating Factors; D014409:Tumor Necrosis Factor-alpha; D002686:Chitin; D007372:Interferons