Development of an anti-idiotypic antibody that blocks substance P primary antibodies and substance P membrane binding.
Polyclonal antibodies against substance P were raised in rabbits and partially purified. This apparently homogeneous immunoglobulin fraction was used to immunize other rabbits. The anti-idiotypic antibodies derived from these rabbits were substantially more effective in competing with substance P than in competing with beta-endorphin for binding to their respective primary antibodies. The anti-idiotypic antibody was also 50 times more potent in competing with substance P binding than in competing with substance K binding to rat duodenal membranes, a tissue containing receptors for substance P and substance K. The anti-idiotypic antibodies exhibited significant enhancement of substance P induced spasmogenic response on the rat uterus and guinea pig ileum (GPI). The results indicate that it is possible to develop anti-idiotypic antibodies that recognize substance P receptors. These antibodies will be of value in studies of the physiological roles of the neuropeptide, substance P.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis* / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006168:Guinea Pigs; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D018040:Receptors, Neurokinin-1; D017981:Receptors, Neurotransmitter / Q000276:immunology*; D013373:Substance P / Q000276:immunology* / Q000378:metabolism; D013388:Succinimides / Q000378:metabolism
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; C043194:Bolton Hunter reagent-substance P conjugate; D007130:Immunoglobulin Idiotypes; D018040:Receptors, Neurokinin-1; D017981:Receptors, Neurotransmitter; D013388:Succinimides; D013373:Substance P