Methods for the determination of the interferon-induced enzyme 2'-5' oligoadenylate synthetase in mononuclear blood cells.
The determination of 2'-5'-oligoadenylate synthetase in peripheral blood mononuclear cells is used as a biological response parameter during therapy with interferon and in the diagnosis of diseases related to the interferon system. In this communication, some general aspects concerning the preparation of 2'-5'-oligoadenylate synthetase from peripheral blood mononuclear cells and the incubation conditions of the 2'-5'-oligoadenylate synthetase reaction are reported. Four analytical procedures for the determination of the products formed during the 2'-5'-oligoadenylate synthetase reaction were comparatively investigated and the advantages and limitations of the assays are discussed. As an example of possible clinical application, the levels of 2'-5'-oligoadenylate synthetase were determined in the mononuclear cell fraction of peripheral blood from healthy persons as well as from children with chronic myelogenous leukaemia.
D015088:2',5'-Oligoadenylate Synthetase / Q000096:biosynthesis / Q000097:blood*; D002846:Chromatography, Affinity; D004790:Enzyme Induction / Q000187:drug effects; D005069:Evaluation Studies as Topic; D006801:Humans; D066298:In Vitro Techniques; D007372:Interferons / Q000494:pharmacology*; D007951:Leukemia, Myeloid / Q000201:enzymology; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D011070:Poly I-C / Q000378:metabolism
D007372:Interferons; D015088:2',5'-Oligoadenylate Synthetase; D011070:Poly I-C