Uniform chromatographic conditions for quantifying urinary catecholamines, metanephrines, vanillylmandelic acid, 5-hydroxyindoleacetic acid, by liquid chromatography, with electrochemical detection.
Uniform liquid-chromatographic conditions were developed such that we could quantify norepinephrine, epinephrine, normetanephrine, metanephrine, vanillylmandelic acid, and 5-hydroxyindoleacetic acid in urine by using a single mobile phase of monochloroacetic acid and citric acid, 0.1 mol/L each. All compounds were separated on a C18 column and detected electrochemically at a potential of +0.800 V. Optimization of these uniform chromatographic conditions significantly shortens the changeover time required from one assay to another, resulting in a substantial savings of time and cost to the laboratory.
D002395:Catecholamines / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D004837:Epinephrine / Q000031:analogs & derivatives*; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000652:urine*; D008676:Metanephrine / Q000652:urine*; D013997:Time Factors; D014642:Vanilmandelic Acid / Q000652:urine*
D002395:Catecholamines; D008676:Metanephrine; D006897:Hydroxyindoleacetic Acid; D014642:Vanilmandelic Acid; D004837:Epinephrine