title: Maintenance of expression of differentiated function of kidney cells following transformation by SV40 early region DNA.
abstract: This study describes the isolation and characterization of epithelial cell lines that maintain their differentiated phenotype following the stable integration of SV40 genes. Epithelial cells were derived from a defined location of rabbit kidney, the thick ascending limb of Henle's loop, and were co-transfected with genes from the early region of SV40 together with pSV2-neo DNA (which confers resistance to the antibiotic G418). These cells were shown to be resistant to G418, express SV40 large T-antigen and continued to express differentiated characteristics typical of cells of their origin. Such characteristics include the expression of high levels of activity of both Na,K-ATPase and the functionally important Na,K,Cl-co-transport system, the synthesis of Tamm-Horsfall glycoprotein and the presence of a barium-sensitive K+ channel on the apical membrane surface.
mesh_terms: D000818:Animals; D000957:Antigens, Viral, Tumor; D002352:Carrier Proteins / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics; D004351:Drug Resistance; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005814:Genes, Viral; D005839:Gentamicins / Q000494:pharmacology; D007473:Ion Channels / Q000378:metabolism; D007684:Kidney Tubules / Q000166:cytology*; D008138:Loop of Henle / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D009088:Mucoproteins / Q000096:biosynthesis; D011188:Potassium / Q000378:metabolism; D011817:Rabbits; D013539:Simian virus 40 / Q000235:genetics* / Q000276:immunology; D028021:Sodium-Potassium-Chloride Symporters; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014162:Transfection; D058949:Uromodulin
chemical_list: D000957:Antigens, Viral, Tumor; D002352:Carrier Proteins; D004279:DNA, Viral; D005839:Gentamicins; D007473:Ion Channels; D009088:Mucoproteins; D028021:Sodium-Potassium-Chloride Symporters; D058949:Uromodulin; C010680:antibiotic G 418; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium