Absence of cytokeratin in human hepatoma cell lines.
The immunofluorescence study revealed that both our established human hepatoma cell lines, HA22T/VGH and HA47T/VGH, were absent of cytokeratin. This observation was further confirmed by a western blot study. However, they as well as the other human hepatoma cells, Hep G2, Hep 3B, and SK-Hep-1 expressed vimentin.
D006528:Carcinoma, Hepatocellular / Q000032:analysis*; D002460:Cell Line; D003599:Cytoskeleton / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D006801:Humans; D007118:Immunoassay; D007382:Intermediate Filaments / Q000032:analysis*; D007633:Keratins / Q000032:analysis*; D008113:Liver Neoplasms / Q000032:analysis*; D014746:Vimentin / Q000032:analysis
D014746:Vimentin; D007633:Keratins