Photometric determination of phospholipase A.
A photometric method for the determination of phospholipase A is described, in which fatty acids are liberated from phosphatidylcholine and measured by a discontinuous enzymatic test. Optimal test conditions were evaluated using purified phospholipase A from human and porcine pancreas. The enzyme activities measured in normal human sera are in the range of 0 to 10 U/l. Pathological values reach more than 100 U/l. There is no correlation with the activities of lipase or amylase in cases of acute pancreatitis.
D000681:Amylases / Q000032:analysis / Q000097:blood; D000818:Animals; D003840:Deoxycholic Acid / Q000032:analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008049:Lipase / Q000032:analysis / Q000097:blood; D010179:Pancreas / Q000201:enzymology*; D010195:Pancreatitis / Q000201:enzymology; D010740:Phospholipases / Q000032:analysis*; D010741:Phospholipases A / Q000032:analysis* / Q000097:blood; D010783:Photometry; D013552:Swine
D003840:Deoxycholic Acid; D010740:Phospholipases; D008049:Lipase; D010741:Phospholipases A; D000681:Amylases