title: [Accumulation of the infectious virus and the viral antigen during the multiplication of the hepatitis A virus in an embryonic kidney cell culture of the rhesus macaque (FRhK-4)].
abstract: Growth characteristics of the HAS-15 strain of human hepatitis A virus (HAV) in rhesus monkey foetal kidney cell line (FRhK-4) are described. The conditions optimal for the accumulation of infectious HAV and viral antigen (HAAg) in the infected cells and tissue culture fluids were studied. The production of infectious HAV occurred in the first stage while in the second stage predominantly HAAg was accumulating intracellularly. Serological properties of the cultivated HAV proved to be very similar to those of the HAV occurring in hepatitis A patients.
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D000939:Epitopes / Q000032:analysis; D006507:Hepatovirus / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D013997:Time Factors; D014776:Virus Cultivation / Q000379:methods; D014779:Virus Replication
chemical_list: D000956:Antigens, Viral; D000939:Epitopes
vernacular_title: Nakoplenie infektsionnogo virusa i virusnogo antigena pri razmnozhenii virusa gepatita A v kul'ture kletok pochki émbriona makaki rezusa (FRhK-4).