Immunocytochemical demonstration of ornithine decarboxylase in the rat exocrine pancreas using the protein A-gold technique.
Previous studies from our laboratory have shown that caerulein, a cholecystokinin analog, can induce pancreatic growth. Because ornithine decarboxylase (ODC) could be involved in this process, it is of interest to localize and estimate ODC immunoreactivity in rat pancreatic acinar cells from control and caerulein-treated animals. This was carried out with the protein A-gold immunocytochemical technique. Rats received either saline (control) or caerulein at a dose of 1 microgram X kg-1 and were sacrificed 8 h after the first injection (control and caerulein group), 4 h after the second caerulein injection (second caerulein group), and 8 h after the third caerulein injection (third caerulein group). ODC immunoreactivity was revealed using a specific antibody. ODC was localized specifically in nuclei and rough endoplasmic reticulum (RER) of the pancreatic acinar cells and the number of gold particles was increased in both of these organelles by caerulein. Peak ODC immunoreactivity was observed in nuclei 4 h after the second caerulein injection, whereas it occurred 8 h after the third peptide injection in the RER. These studies are the first to demonstrate ODC localization in pancreatic acinar cells and show that the enzyme can be induced early upon growth stimulation of the organ by a cholecystokinin analog.
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D002108:Ceruletide / Q000494:pharmacology; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D006046:Gold; D006651:Histocytochemistry; D007700:Kinetics; D008297:Male; D008854:Microscopy, Electron; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D010179:Pancreas / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling; D013205:Staphylococcal Protein A
D013205:Staphylococcal Protein A; D006046:Gold; D002108:Ceruletide; D009955:Ornithine Decarboxylase