[Method of determining proteolytic activity by using a conjugate of bovine serum albumin with peroxidase].
The authors propose a method for determination of proteolytic activity, based on the hydrolysis of peroxidase-labeled molecules of bovine serum albumin immobilized on the surface of polystyrene microassay plates with the subsequent determination of peroxidase activity on the carrier or in the solution. The optimum conditions for the sorption of the labeled substrate have been established. The method permits the determination of bacillary alkaline protease at a concentration of 01. microgram/ml within 45 minutes. The determination of four proteases has demonstrated that this method shows good correlation with the routine one (r = 0.98), but is more sensitive and less time- and labor-consuming.
D000681:Amylases / Q000032:analysis; D001230:Aspergillus / Q000201:enzymology; D001412:Bacillus subtilis / Q000201:enzymology; D002850:Chromatography, Gel; D004338:Drug Combinations / Q000032:analysis; D004792:Enzyme Precursors / Q000032:analysis; D006735:Horseradish Peroxidase; D006868:Hydrolysis; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D010447:Peptide Hydrolases / Q000032:analysis*; D010544:Peroxidases; D012710:Serum Albumin, Bovine; D014357:Trypsin / Q000032:analysis
D004338:Drug Combinations; D004792:Enzyme Precursors; D012710:Serum Albumin, Bovine; C009402:amylase, phosphates, proteases drug combinations; C040620:protosubtilin; D006735:Horseradish Peroxidase; D010544:Peroxidases; D000681:Amylases; D010447:Peptide Hydrolases; D014357:Trypsin
Metod opredeleniia proteoliticheskoĭ aktivnosti s ispol'zovaniem kon''iugata bych'ego syvorotochnogo al'bumina s peroksidazoĭ.