Homology among beta-crystallins: monoclonal antibodies to beta-heavy crystallin.
The mouse monoclonal antibodies, prepared with rat beta H-crystallin as parent antigen, show cross-reactivity to specific subunits in beta H-, beta L1-, and beta L2-crystallin but no reactivity to either alpha- or gamma-crystallins. Antigenic homology among the beta-crystallin subunits was demonstrated by comparison of the topographic distributions of peptides on two-dimensional electrophoretograms subjected to either staining or an ELISA of the immunoblots.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000302:isolation & purification; D003429:Cross Reactions; D003459:Crystallins / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D007122:Immunoelectrophoresis; D051381:Rats; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal; D003459:Crystallins; D000939:Epitopes