Role of adherent cells in dextran sulfate-mediated enhancement of mitogen-induced B-cell proliferation.
We have studied lipopolysaccharide (LPS)-induced proliferation in the rat and have found that the addition of the compound Dextran sulfate (DxS), which itself is not mitogenic, to LPS stimulated cultures results in significant enhancement of cell division. A "DxS-free" supernatant from DxS stimulated spleen cell cultures is able to substitute for DxS in stimulatory activity. This supernatant possesses interleukin 1 (IL-1) activity, however, the addition of purified recombinant IL-1 to LPS-stimulated cultures does not result in augmentation of proliferation. A DxS-free supernatant from DxS stimulated adherent cells is also able to substitute for DxS in stimulatory activity. The active molecule(s) present in the adherent cell-derived DxS-free culture supernatant appears to be distinct from classical IL-1.
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D003470:Culture Media; D016264:Dextran Sulfate; D003911:Dextrans / Q000494:pharmacology*; D005260:Female; D007375:Interleukin-1 / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008264:Macrophages / Q000276:immunology*; D008934:Mitogens; D051381:Rats; D011917:Rats, Inbred Lew; D011994:Recombinant Proteins / Q000494:pharmacology
D003470:Culture Media; D003911:Dextrans; D007375:Interleukin-1; D008934:Mitogens; D011994:Recombinant Proteins; D016264:Dextran Sulfate