An ELISA method for quantification of Tamm-Horsfall protein using monoclonal antibodies.
Eight hybridoma cell lines secreting monoclonal antibodies (MoAbs) to Tamm-Horsfall protein (THP) were established. The isotype and reaction pattern of the MoAbs with THP from rat, rabbit, guinea pig and man were employed for the selection of clones. At least four epitopes were recognised on human THP. One of these epitopes differed from the others in its dependence on the state of aggregation of the THP. An ELISA procedure was developed for quantification of THP in urine requiring no other sample treatment than dilution in the assay buffer. In this ELISA, THP showed an increased immunoreactivity after freezing.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000302:isolation & purification; D002846:Chromatography, Affinity / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D006168:Guinea Pigs; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D051379:Mice; D009088:Mucoproteins / Q000276:immunology / Q000652:urine*; D009994:Osmolar Concentration; D011817:Rabbits; D013045:Species Specificity; D058949:Uromodulin
D000911:Antibodies, Monoclonal; D000939:Epitopes; D009088:Mucoproteins; C548612:UMOD protein, human; C548613:Umod protein, mouse; C548614:Umod protein, rat; D058949:Uromodulin