Monoclonal antibody binding to factor VIII:c.
The interactions of human blood clotting Factor VIII:c with 2 monoclonal antibodies, C7F7 and BD10, were examined with an enzyme-linked immunosorbent assay (ELISA) method. A Scatchard-Sips plot approach to data analysis was used to calculate an average affinity (Ko), valence (n), and heterogeneity index (a) for each interaction. Using a Factor VIII:c coating ELISA method, the following constants were determined: BD10: Ko = 5.48 X 10(8) M-1; n = 0.31; a = 0.97; C7F7: Ko = 2.3 X 10(11) M-1; n = 0.018; a = 0.77.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D005169:Factor VIII / Q000276:immunology*; D006801:Humans; D007700:Kinetics
D000911:Antibodies, Monoclonal; D000939:Epitopes; D005169:Factor VIII