Insulin and glyburide increase cytosolic free-Ca2+ concentration in isolated rat adipocytes.
We investigated the effect of insulin and a hypoglycemic sulfonylurea agent glyburide on cytosolic free-Ca2+ concentrations [( Ca2+]i) in isolated rat adipocytes. Both insulin and glyburide increased [Ca2+]i in a dose-dependent manner. Half-maximal effects were seen at 0.5 ng/ml of insulin and 0.5 microM glyburide. Nifedipine (25 microM), a Ca2+-channel blocker, inhibited the effect of both agents. The effect of insulin on [Ca2+]i was 40 and 70% potentiated by ambient glucose concentrations at 180 and 300 mg/dl, respectively. Depolarizing doses of potassium (40 mM) induced an increase in cytosolic Ca2+ that was also inhibited by nifedipine. It is suggested that both insulin and glyburide increase cytosolic free Ca2+ levels at least in part by promoting Ca2+ influx through voltage-dependent Ca2+ channels.
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D005905:Glyburide / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D007473:Ion Channels / Q000187:drug effects*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D007328:Insulin; D007473:Ion Channels; D005905:Glyburide; D002118:Calcium