Production of tumor-specific suppressor T cell hybridomas.
Spleen cells from late tumor-bearing host mice (TBH) with progressive P815 tumors have been shown to suppress the generation of cytotoxic T lymphocytes (CTL) from spleens of syngeneic immune or early TBH mice in vitro. This suppression is antigen-specific and is mediated by T lymphocytes. By positive selection, a highly enriched population of T cells was obtained from late TBH spleens and fused with the BW5147 HAT-sensitive thymoma. Cloned hybrid cell lines were obtained which released soluble antigen-specific factors capable of suppressing anti-P815 CTL generation. Similar T cell hybrids with suppressive activity were also derived from the fusion of spleen cells of TBHs bearing a HAT-sensitive subline of the P815 tumor.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology; D000939:Epitopes; D006825:Hybridomas / Q000276:immunology*; D008223:Lymphoma / Q000276:immunology; D012515:Mast-Cell Sarcoma / Q000276:immunology; D051379:Mice; D008811:Mice, Inbred DBA; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000939:Epitopes