Interferon-beta inhibition of concanavalin A-stimulated calcium uptake and exchange in HeLa cells.
Addition of the lectin concanavalin A (ConA) to HeLa-S3 tumor cells in culture stimulates calcium exchange and uptake. Pretreatment of the cells with interferon-beta (IFN-beta) at a concentration of 640 U/ml for 24 h markedly inhibits this ConA-stimulated calcium exchange and uptake, but not the basal level of calcium exchange. These findings suggest that a number of IFN effects on cell function may reflect an impaired ability of IFN-treated cells to respond to molecules such as growth factors and peptide hormones with increased calcium fluxes.
D002118:Calcium / Q000378:metabolism*; D002132:Calcium Radioisotopes; D003208:Concanavalin A / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D006367:HeLa Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D007473:Ion Channels / Q000187:drug effects; D013997:Time Factors
D002132:Calcium Radioisotopes; D007473:Ion Channels; D003208:Concanavalin A; D007371:Interferon-gamma; D002118:Calcium