Determination of serotonin and 5-hydroxyindoleacetic acid in guinea pig and human prostate using HPLC.
The finding of significant numbers of endocrine-paracrine (EP) cells in the prostate glands of guinea pigs and man suggests that these cells may be important in the regulation or modulation of prostatic function. Serotonin is a biogenic amine common to most prostatic EP cells. In order to extend current knowledge relating to these cells, an assay was developed using high-performance liquid chromatography to quantitate serotonin and its metabolite 5-hydroxyindoleacetic acid (5-HIAA) in guinea pig and human prostatic tissue extracts. Levels of serotonin and 5-HIAA in the guinea pig whole-gland preparation were 105.4 +/- 70.6 ng/g and 48.4 +/- 95.7 ng/g, respectively. Normal human prostatic tissue contained 1423.9 +/- 750.8 ng/g serotonin and 66.7 +/- 92.8 ng/g 5-HIAA. Recoveries ranged between 60 and 100%. The detection limits were 24 pg/injection for serotonin and 12 pg/injection for 5-HIAA. This assay provides an expeditious, specific and highly sensitive method for the simultaneous determination of monoamines in guinea pig and human prostatic tissue.
D000368:Aged; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004298:Dopamine / Q000031:analogs & derivatives / Q000032:analysis; D006168:Guinea Pigs; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D011467:Prostate / Q000032:analysis*; D012701:Serotonin / Q000032:analysis*
D012701:Serotonin; C022133:3,4-dihydroxybenzylamine; D006897:Hydroxyindoleacetic Acid; D004298:Dopamine