Evidence for a change in molecular for of DNA ligase in early development of the sea urchin Psammechinus miliaris.
A change in the molecular form of DNA ligase appears when the sea urchin egg enters cleavage. Sucrose gradient analysis and DNA cellulose chromatography show that a slower migrating form (7 S) of enzyme exists in unfertilized eggs and in sperm. A faster migrating form of DNA ligase (7.8 S) is present in developing embryos as well as in artificially activated eggs. The timing of this early biochemical event has been determined, following fertilization or activation. The change in molecular form of DNA ligase has been shown to be sensitive to drugs inhibiting protein synthesis, gene transcription, or DNA replication. Consequently the appearance of the faster migrating form of enzyme is assumed to result from expression of the corresponding gene, transcription, and translation. RNA extracted from testes and from cleaving stages, assayed in vitro and in vivo, have been shown to carry the information for, respectively, 7 S and 7.8 S DNA ligase according to the origin of the RNA.
D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D002499:Centrifugation, Density Gradient; D002848:Chromatography, DEAE-Cellulose; D003513:Cycloheximide / Q000494:pharmacology; D011088:DNA Ligases / Q000378:metabolism*; D011118:Polynucleotide Ligases / Q000378:metabolism*; D014176:Protein Biosynthesis; D012313:RNA / Q000378:metabolism; D012617:Sea Urchins; D013997:Time Factors; D015053:Zygote / Q000201:enzymology*
D000546:Amanitins; D012313:RNA; D003513:Cycloheximide; D011088:DNA Ligases; D011118:Polynucleotide Ligases