Monoclonal antibody G 250 recognizes a determinant present in renal-cell carcinoma and absent from normal kidney.
This report describes a monoclonal antibody designated G 250, subclass IgGl, that recognizes an antigen preferentially expressed on cell membranes of renal-cell carcinoma cells (RCC) and not expressed in normal proximal tubular epithelium. G 250 antibody reacted with 46 of 47 primary RCC, with 7 of 8 RCC metastases and with a few other malignant tumors. The staining pattern of G 250 differs from that of other RCC-related antibodies described. Preliminary experiments show that this antibody can be used to visualize RCC xenografts in nude mice by immunoscintigraphy.
D000818:Animals; D000911:Antibodies, Monoclonal; D002292:Carcinoma, Renal Cell / Q000276:immunology*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D004848:Epithelium / Q000276:immunology; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D007668:Kidney / Q000276:immunology*; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000276:immunology; D051379:Mice; D008819:Mice, Nude; D011877:Radionuclide Imaging; D014018:Tissue Distribution; D014183:Transplantation, Heterologous
D000911:Antibodies, Monoclonal; D000939:Epitopes