Quantitation of myelination and demyelination by the measurement of myelin basic protein by ELISA.
Utilizing a competitive inhibition enzyme-linked immunosorbent assay (ELISA) we measured the amount of myelin basic protein (MBP) in brain, liver, and kidney of newborn mouse and compared these values with myelinated cerebellar explant cultures under various conditions. Explant cultures demyelinated with anti-myelin serum showed a 90% decrease in MBP when compared with controls. These results correlated with myelination and demyelination as observed by light microscopy. Newborn tissues including brain showed only negligible activity for MBP. This technique provides a sensitive and objective method for quantitating in-vitro demyelination.
D000818:Animals; D000831:Animals, Newborn; D002531:Cerebellum / Q000032:analysis*; D046508:Culture Techniques; D003711:Demyelinating Diseases / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D007668:Kidney / Q000032:analysis; D008099:Liver / Q000032:analysis; D051379:Mice; D004676:Myelin Basic Protein / Q000032:analysis*; D011863:Radioimmunoassay
D004676:Myelin Basic Protein