Comparison of HLA-Dw1 and -Dw2 positive adherent cells in antigen presentation to heterozygous T-cell lines: a low rubella antigen-specific response associated with HLA-Dw2.
T-cell lines specific for purified tuberculin (PPD), rubella, and herpes simplex virus (HSV) antigens were established from two Dw1/w2 heterozygous subjects. Fresh peripheral blood monocytes from healthy individuals with shared and non-shared D-antigens were used as presenting cells and the T-cell lines as responders in lymphocyte blast transformation assays with specific antigens. An HLA-restricted reaction was seen with all the antigens, although the PPD- and HSV-specific cell lines reacted stronger than rubella-specific lines when the specific antigens were presented by monocytes not sharing the D-antigens. A significantly lower response was seen when rubella antigen was presented by Dw2 positive monocytes as compared to response promoted by Dw1 positive monocytes (p less than 0.0001, Mann-Whitney U-test), whereas no such difference was seen in responses to PPD or herpes simplex antigen.
D000938:Antigen-Presenting Cells / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D002448:Cell Adhesion; D002460:Cell Line; D000939:Epitopes / Q000032:analysis* / Q000276:immunology; D006681:HLA-D Antigens / Q000276:immunology*; D006579:Heterozygote; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation; D012412:Rubella virus / Q000276:immunology*; D018139:Simplexvirus / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014373:Tuberculin / Q000276:immunology
D000956:Antigens, Viral; D000939:Epitopes; D006681:HLA-D Antigens; D000949:Histocompatibility Antigens Class II; D014373:Tuberculin