Use of cultured epithelial cells, including keratinocytes, for the detection of antinuclear antibodies.
The use of cultured human epithelial cells as a substrate for the detection of antinuclear antibodies (ANAs) improves the sensitivity of detection above the standard substrates used. Standard antisera have been screened against a series of epithelial cell lines, including keratinocytes. Selected cells have then been used to detect ANAs in 78 patients with discoid lupus erythematosus.
D000974:Antibodies, Antinuclear / Q000032:analysis*; D001681:Biological Assay; D002460:Cell Line; D000078404:Epidermal Cells; D004817:Epidermis / Q000276:immunology; D004848:Epithelium / Q000276:immunology; D006801:Humans; D007633:Keratins / Q000276:immunology*; D008179:Lupus Erythematosus, Discoid / Q000276:immunology*
D000974:Antibodies, Antinuclear; D007633:Keratins