Cyclosporin A has differential effects on the responses of murine B cells to TI antigens and B-cell mitogens.
The effect of cyclosporin A (CyA) on the response of murine splenocytes to B-cell mitogens, TI-1 and TI-2 antigens was investigated. The proliferative response to LPS was found to be four- to five-fold less sensitive to inhibition than that to dextran sulphate. Antibody responses to a TI-2 antigen in vivo were suppressed by CyA treatment, whereas responses to the TI-1 antigen DNP-LPS were markedly enhanced. Enhanced antibody responses to DNP-LPS were also demonstrable in vitro in the presence of CyA, and the enhancement was not removed by T-cell depletion. LPS-induced antibody production in vitro was enhanced at the same CyA concentrations that inhibited proliferation by 40-50%. The implications of these findings for the mechanism of action of CyA and for our understanding of B-cell differentiation are discussed.
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D000955:Antigens, T-Independent / Q000276:immunology*; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D003524:Cyclosporins / Q000494:pharmacology*; D016264:Dextran Sulfate; D003911:Dextrans / Q000494:pharmacology; D005260:Female; D005362:Ficoll / Q000031:analogs & derivatives / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D008938:Mitosis / Q000187:drug effects
D000955:Antigens, T-Independent; D003524:Cyclosporins; C031007:DNP-Ficoll; D003911:Dextrans; D008070:Lipopolysaccharides; D005362:Ficoll; D016264:Dextran Sulfate