title: Establishment and characterization of two new squamous cell carcinoma cell lines derived from tumors of the head and neck.
abstract: Two human cell lines were established from untreated squamous cell carcinomas of the head and neck. Line 183 was derived from a head and neck squamous cell carcinoma of the tonsil and 1483 from a head and neck squamous cell carcinoma of the retromolar trigone. Both lines grow in a cobblestone pattern demonstrating their epithelial heritage. Immunofluorescence studies and one-dimensional polyacrylamide gel electrophoresis indicated that both lines contain cytokeratins. Line 1483 is more aggressive in nude mice, has a higher efficiency for anchorage-independent growth, expresses p21ras (product of the ras oncogene) at a higher level, and is more aneuploid than 183. 1483 also grows as a multicellular tumor spheroid. Line 1483, which was established from the primary tumor of a patient with nodal metastasis, thus displays more progressed characteristics than line 183, which was established from a patient with no clinically positive nodes.
mesh_terms: D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000032:analysis / Q000235:genetics / Q000473:pathology*; D002449:Cell Aggregation; D002869:Chromosome Aberrations; D005819:Genetic Markers; D006258:Head and Neck Neoplasms / Q000032:analysis / Q000235:genetics / Q000473:pathology*; D006801:Humans; D007633:Keratins / Q000032:analysis; D008297:Male; D008875:Middle Aged; D011518:Proto-Oncogene Proteins / Q000032:analysis; D016283:Proto-Oncogene Proteins p21(ras); D014407:Tumor Cells, Cultured
chemical_list: D005819:Genetic Markers; D011518:Proto-Oncogene Proteins; D007633:Keratins; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)