Redistribution of protein kinase C in pancreatic acinar cells stimulated with caerulein or carbachol.
We examined phospholipid/calcium-dependent protein kinase (protein kinase C) activity and amylase secretion in isolated pancreatic acinar cells, when exposed to caerulein or carbachol. Upon stimulation with 10(-10) M caerulein or 10(-6) M carbachol cytosolic protein kinase C activity was increased in accordance with amylase secretion. Effect of carbachol on increase in membrane-associated protein kinase C activity was maximal at 10(-6) M where the rate of amylase secretion was highest. On the other hand, caerulein showed the maximal secretion of amylase at 10(-9) M, but the activity of the protein kinase C associated with membranes increased progressively with increasing concentration of caerulein. These results indicate different profiles of redistribution of protein kinase C upon stimulation of pancreatic acinar cells with carbachol or caerulein, and they were discussed in terms of amylase secretion.
D000681:Amylases / Q000378:metabolism; D000818:Animals; D029742:Caenorhabditis elegans Proteins; D002217:Carbachol / Q000494:pharmacology*; D002273:Carcinogens / Q000378:metabolism; D002352:Carrier Proteins; D002458:Cell Fractionation; D002462:Cell Membrane / Q000378:metabolism; D002108:Ceruletide / Q000494:pharmacology*; D003600:Cytosol / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology*; D015240:Phorbol 12,13-Dibutyrate; D010703:Phorbol Esters / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D011955:Receptors, Drug; D011971:Receptors, Immunologic / Q000378:metabolism; D013347:Subcellular Fractions / Q000201:enzymology
D029742:Caenorhabditis elegans Proteins; D002273:Carcinogens; D002352:Carrier Proteins; D010703:Phorbol Esters; D011955:Receptors, Drug; D011971:Receptors, Immunologic; C083141:phorbol ester binding protein; C027776:phorbol ester receptor; D015240:Phorbol 12,13-Dibutyrate; D002108:Ceruletide; D002217:Carbachol; D011493:Protein Kinase C; D000681:Amylases