title: A qualitative and quantitative assessment of the humoral antibody response of the sheep to orf virus infection.
abstract: The serological response of naturally and experimentally infected lambs to orf virus infection was analysed using an enzyme-linked immunosorbent assay (ELISA) together with the Western blotting technique. The combination of these two methods permitted a qualitative and quantitative assessment of the response, which revealed considerable variation between animals. Despite this, all post-exposure sera reacted with a polypeptide (molecular weight 40 kilodaltons) which appears to be a component of the surface tubules that are characteristic of the virus.
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D000956:Antigens, Viral / Q000276:immunology*; D004474:Ecthyma, Contagious / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D007118:Immunoassay; D007700:Kinetics; D009923:Orf virus / Q000276:immunology*; D011212:Poxviridae / Q000276:immunology*; D012756:Sheep; D013047:Specific Pathogen-Free Organisms
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes