The non-mature sperm cells: evaluation of surface markers and interaction in in vitro cultures.
The ejaculated human seminal plasma cells may be classified into subpopulations on the basis of spermatogenesis or biological features. In this paper we divided these cells into two main groups: one of them is obviously predestinated to fertilization as mature sperm cells, viable and motile ejaculated cells. Second group of unknown biological role is very heterogeneous; simplifying its variety we call them non-mature sperm cells. Morphological studies by electron microscopy and evaluation of surface markers of these cells by monoclonal antibodies did not reveal any known biological features or markers for cells of leukocyte origin. They did not express any antigenic determinants of HLA system and they did not react with autologous or allogeneic lymphocytes.
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000032:analysis*; D002478:Cells, Cultured; D000939:Epitopes / Q000032:analysis; D006680:HLA Antigens / Q000032:analysis; D006801:Humans; D008213:Lymphocyte Activation; D008297:Male; D008854:Microscopy, Electron; D009845:Oligospermia / Q000503:physiopathology; D011863:Radioimmunoassay; D013091:Spermatogenesis; D013094:Spermatozoa / Q000254:growth & development / Q000276:immunology* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D000939:Epitopes; D006680:HLA Antigens