The beta subunit of the guanine nucleotide regulatory proteins: identification of its location on two-dimensional gels of brain tissue and its regional and subcellular distribution in brain.
The beta subunit of the guanine nucleotide regulatory proteins (also termed G proteins) has been examined in both rat and human brain. Proteins contained within samples of fresh rat and human brain tissue were separated by two-dimensional gel electrophoresis and either stained with silver or reacted with various antisera raised against the G proteins. In both rat and human brain, a single protein of molecular weight 36,000 daltons and pI 5.8 reacted the antisera. This protein also comigrated with one of the proteins present in a purified preparation of bovine brain G proteins. Based upon molecular weight, pI, and reaction with specific antisera, it was concluded that this protein is the beta subunit of the G proteins in brain. Using this information, the regional and subcellular distribution of the G protein beta subunit was studied in rat brain. Of 25 distinct neuroanatomical areas examined, cortical regions were generally found to contain the largest amount of this protein. The subcellular distribution of the G protein beta subunit revealed that large amounts are present in the synaptic membrane, crude synaptic vesicles, and microsomes. These studies serve to identify another protein visible on silver-stained two-dimensional electrophoretograms of rat and human brain. The regional and subcellular distribution of the G protein beta subunit correlate well with the proposed physiological function of this protein.
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002540:Cerebral Cortex / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D019204:GTP-Binding Proteins / Q000032:analysis*; D006801:Humans; D007158:Immunologic Techniques; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012834:Silver; D013194:Staining and Labeling; D013347:Subcellular Fractions / Q000032:analysis
D012834:Silver; D019204:GTP-Binding Proteins