A possible correlation between DA-DAPI counterstaining and alu I-induced bands in fixed human chromosomes.
DA-DAPI counterstaining is a technique which reveals the heterochromatic areas of chromosomes 1, 9, 15, 16 and Y in humans; a result which may be related to the peculiar characteristics of these chromosomal regions, particularly regarding their DNA content. The present report describes a different DA-DAPI stain reaction. Obtained under standard conditions, it shows positive, bright staining in a large number of chromosomes. Such a result is discussed in relation to the banding pattern produced by Alu I in human metaphase chromosomes, in view of the high degree of overlapping of the two patterns.
D002871:Chromosome Banding / Q000379:methods*; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; D004214:Distamycins; D005404:Fixatives; D006801:Humans; D007211:Indoles; D008677:Metaphase; D011758:Pyrroles; D013194:Staining and Labeling
D004214:Distamycins; D005404:Fixatives; D007211:Indoles; D011758:Pyrroles; C007293:DAPI; C030000:stallimycin; D004262:DNA Restriction Enzymes; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific