[Thermal inactivation and stabilization of lysozyme substrate-- Micrococcus lysodeicticus cells].
Heat inactivation of the acetonic powder of Micrococcus lysodeicticus cells suspended in phosphate buffer pH 6.2 was quantitatively characterized in the temperature range from 34 to 52 degrees. The total value of the rate constant for heat inactivation of the cells equals 2.88 X 10(8) exp(-18360/RT) sec-1. The activation parameters of the process at 34 degrees are the following: delta H* = 17.7 kcal/mole; delta S* = 21.8 E. U.; delta F* = 24.4 kcal/mole. The effect of ethylene glycol, mannitol, dextran, polyvinyl alcohol (PVA) and polyethylene glycols with different molecular weights on the lysis rate and cell stability was studied. Polyvinyl alcohol was found to be the most effective stabilizer. At concentrations of about 10(-5) it enhances the thermostability of the cells threefold.
D003911:Dextrans / Q000494:pharmacology; D006358:Hot Temperature; D007700:Kinetics; D008837:Micrococcus / Q000187:drug effects / Q000378:metabolism*; D009113:Muramidase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000494:pharmacology; D011142:Polyvinyl Alcohol / Q000494:pharmacology; D013379:Substrate Specificity
D003911:Dextrans; D011092:Polyethylene Glycols; D011142:Polyvinyl Alcohol; D009113:Muramidase
Termicheskaia inaktivatsiia i stabilizatsiia substrata lizotsima-- kletok Micrococcus lysodeicticus.