A monoclonal antibody against a synthetic peptide reveals common structures among spectrins and alpha-actinin.
A monoclonal antibody (Mab) against a synthetic peptide, SEDYGKDL, corresponding to one conserved sequence in the chicken alpha-fodrin repeats reacts in immunoblotting with avian alpha-spectrin and alpha-fodrin, both mammalian spectrins and with mammalian alpha-fodrin. This Mab also reacts with alpha-actinin in both chicken and human cells. Our results confirm the previously detected structural homology between spectrins and alpha-actinin and implicate their common evolutionary origin.
D000185:Actinin / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002352:Carrier Proteins / Q000276:immunology; D002642:Chick Embryo; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D006801:Humans; D008840:Microfilament Proteins / Q000276:immunology; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D010802:Phylogeny; D012689:Sequence Homology, Nucleic Acid; D013049:Spectrin / Q000276:immunology*
D000185:Actinin; D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D000939:Epitopes; D008840:Microfilament Proteins; D010455:Peptides; D013049:Spectrin