The assembly of the DNA complex present in chick embryo cell cytosol.
Chromatography of chick embryo fibroblast cytosol labelled with [3H]thymidine or [3H]uridine precursors has shown the presence of early labelled DNA and RNA eluting at a position corresponding to a relative molecular mass of approximately 1.5-10(5). The early DNA-RNA (heteroduplex?) then moves progressively to a higher molecular weight peak, relative molecular mass approximately 10(6). The process appears similar in cytosol from cultured cells and from whole amniotically labelled chick embryos: consequently the cytosolic DNA complex is not an artefact of cell culturing. The relative contribution of artefactual and specific cytosol-associated DNA material is discussed: it is concluded that while both are present in cytosol as prepared, it is possible to discriminate between specific and artefactual DNA material.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D003600:Cytosol / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D008970:Molecular Weight; D009692:Nucleic Acid Heteroduplexes / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D009692:Nucleic Acid Heteroduplexes; D014316:Tritium; D012313:RNA; D004247:DNA; D013936:Thymidine; D014529:Uridine