A rapid method for staining proteins in acrylamide gels.
A negative staining procedure for the rapid visualization of proteins in acrylamide gels is described. In the absence of proteins, staining of the gel occurs through the reduction of nitroblue tetrazolium by reduced glutathione. No staining occurs in the presence of proteins. The procedure can be completed in 20 min and is at least as sensitive as Coomassie brilliant blue staining.
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005978:Glutathione; D009580:Nitroblue Tetrazolium; D010084:Oxidation-Reduction; D011506:Proteins / Q000302:isolation & purification*; D012394:Rosaniline Dyes; D013194:Staining and Labeling / Q000379:methods*
D011506:Proteins; D012394:Rosaniline Dyes; D009580:Nitroblue Tetrazolium; D005978:Glutathione; C004692:coomassie Brilliant Blue