[Non-specific RNA degradation in the presence of magnesium ions].
RNA degradation catalyzed by Mg2+ was studied under the conditions of reverse transcriptase reaction. Agarose gel electrophoresis in 6 M urea was employed to follow the reaction. Natural ribosomal of poly(A)+ RNA as well as synthetic poly(rA) are equally accessible for degradation. Neither an RNAase nor alkali alone is responsible for the degradation. The reaction rate is directly proportional to Mg2+ concentration in the range of 1 to 10 mM, doesn't depend upon RNA concentration and enhances approximately 50 fold upon increasing of pH value from 7.5 to 9.0. It was concluded that the Mg2+ catalyzed degradation reaction is an unspecific one in respect to the primary structure of RNA. The results obtained are useful to optimize the conditions for the reverse transcriptase reaction.
D002478:Cells, Cultured; D004587:Electrophoresis, Agar Gel; D005347:Fibroblasts / Q000032:analysis; D006801:Humans; D006868:Hydrolysis; D008274:Magnesium / Q000494:pharmacology*; D011061:Poly A / Q000032:analysis; D012313:RNA / Q000032:analysis* / Q000187:drug effects
D011061:Poly A; D012313:RNA; D008274:Magnesium
Nespetsificheskaia degradatsiia RNK v prisutstvii ionov magniia.