title: Effect of calcium on histamine release from pleural and peritoneal mast cells induced by catechol.
abstract: Histamine release from rat pleural and peritoneal mast cells induced by catechol (1, 10, 50, 250 microM and 1 mM) has been studied. The dose-response induced by catechol is non-cytotoxic, is not modified by purification of mast cells and is calcium independent. The sensitivity and maximum response to catechol is the same irrespective of the presence or absence of Ca++, except on purified pleural mast cells, that showed a plateau response at 250 microM catechol in the absence of Ca++, and on unpurified peritoneal mast cells which exhibited a lower maximum response equally in the absence of Ca++. The release is induced by catechol at concentrations as low as 50 microM in all cases, and the maximum response is reached at 1 mM.
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002396:Catechols; D005260:Female; D006636:Histamine Release / Q000187:drug effects*; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D010529:Peritoneal Cavity / Q000166:cytology*; D010994:Pleura / Q000166:cytology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D002396:Catechols; D002118:Calcium