title: Enhanced proteolysis of bovine fibrinogen in the presence of polysulfates.
abstract: Proteolysis of fibrinogen by bovine trypsin and chymotrypsin was enhanced by heparin, dextran sulfate (DS) and polyvinyl sulfate (PVS) in the presence of 0.1 M NaCl. Decrease in intrinsic fluorescence of fibrinogen with time was also enhanced by DS and PVS in the absence of NaCl, but not in the presence of 0.1 M NaCl. Thus, increase in susceptibility of fibrinogen to proteases in the presence of three polysulfate was more sensitive than time-dependent conformational change of the substrate protein.
mesh_terms: D000818:Animals; D002417:Cattle; D002918:Chymotrypsin / Q000037:antagonists & inhibitors / Q000378:metabolism; D016264:Dextran Sulfate; D003911:Dextrans / Q000494:pharmacology*; D005340:Fibrinogen / Q000378:metabolism*; D005453:Fluorescence; D006493:Heparin / Q000494:pharmacology*; D006868:Hydrolysis; D007700:Kinetics; D010447:Peptide Hydrolases / Q000378:metabolism*; D011145:Polyvinyls / Q000494:pharmacology*; D012965:Sodium Chloride / Q000494:pharmacology; D014357:Trypsin / Q000378:metabolism
chemical_list: D003911:Dextrans; D011145:Polyvinyls; C019050:polyvinyl sulfate; D012965:Sodium Chloride; D005340:Fibrinogen; D006493:Heparin; D016264:Dextran Sulfate; D010447:Peptide Hydrolases; D002918:Chymotrypsin; D014357:Trypsin