Effect of in vitro (ADP)ribosylation on transcription of the chromatin of the brain of developing rats.
The template-engaged RNA polymerase II was assayed in the nuclei purified from the cerebral hemisphere of 3-, 14- and 30-day old rats. Its activity is the highest at 3-day and declines as development proceeds. Lower transcription at 30-day may either be due to a decrease in the active fraction of chromatin or to a decrease in the amount of RNA polymerase II that is active towards endogenous template, or both. The activity of RNA polymerase I (active in low salt) is also maximal at 3-day and declines as development proceeds. (ADP)ribosylation of chromatin depresses RNA synthesis. This may be due to inactivation of RNA polymerase itself by protein poly(ADP)ribosylation.
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D000375:Aging / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000254:growth & development* / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism*; D008297:Male; D012313:RNA / Q000096:biosynthesis*; D012318:RNA Polymerase I / Q000378:metabolism; D012319:RNA Polymerase II / Q000378:metabolism; D012320:RNA Polymerase III / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014158:Transcription, Genetic
D002843:Chromatin; D000246:Adenosine Diphosphate Ribose; D012313:RNA; D012319:RNA Polymerase II; D012318:RNA Polymerase I; D012320:RNA Polymerase III