CCA paper: a new two-dimensional cyanuric chloride-activated matrix for universal application in molecular biology.
A novel two-dimensional cyanuric chloride-activated (CCA) paper has been developed. It is composed of a cellulosic base, covalently bound cyanuric chloride, and microprecipitated complex cyanuric chloride-sodium chloride crystals on its surface. CCA paper covalently binds nucleic acids and proteins. Its binding capacity for nucleic acids is about 400 micrograms/cm2. Sealed into nitrogen-filled bags and stored at -20 degrees C, it retains its binding activity for at least a year and is always ready for use. CCA paper has been successfully used for capillary and electroblotting of DNA, RNA, and proteins (Southern, Northern, and Western blotting) as well as for dot tests. Furthermore, it was applied to colony and plaque hybridization. A unique property of it is that it permits the staining of proteins after blotting and subsequent performance of radioimmunological detection of specific protein components. This has proven advantageous in two-dimensional Western blotting experiments. Of further importance is its ability to bind DNA fragments from one up to several hundred bp from polyacrylamide sequencing gels.
D000818:Animals; D002417:Cattle; D004247:DNA / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D013058:Mass Spectrometry; D008855:Microscopy, Electron, Scanning; D009693:Nucleic Acid Hybridization; D010209:Paper; D011506:Proteins / Q000302:isolation & purification; D012313:RNA / Q000302:isolation & purification; D013194:Staining and Labeling; D014227:Triazines; D014961:X-Ray Diffraction
D011506:Proteins; D014227:Triazines; C019309:cyanuric chloride; D012313:RNA; D004247:DNA