A method for removal of trace iron contamination from biological buffers.
Laboratory chemicals and reagents normally contain trace amounts of iron salts sufficient to catalyse free radical reactions. This iron contamination can be removed from buffers and reagents using a dialysis sac containing a high-affinity iron-binding protein like conalbumin or transferrin without altering the pH value of the fluid.
D001761:Bleomycin; D002021:Buffers / Q000302:isolation & purification*; D003207:Conalbumin; D003956:Dialysis; D007202:Indicators and Reagents / Q000302:isolation & purification; D007501:Iron / Q000302:isolation & purification*; D014168:Transferrin
D002021:Buffers; D007202:Indicators and Reagents; D014168:Transferrin; D001761:Bleomycin; D003207:Conalbumin; D007501:Iron