Isolation of HIV from Australian patients with AIDS, AIDS related conditions and healthy antibody positive individuals.
The Fairfield Hospital experience with isolation of HIV from peripheral blood leucocytes, cerebrospinal fluid and semen is described. To date HIV has been isolated from single specimens of blood from 45% of patients with AIDS, 35% of patients with lymphadenopathy syndrome AIDS-related complex or ARC and 14% of asymptomatic antibody positive individuals. HIV was recovered from peripheral blood leucocytes in the presence of phytohemagglutinin and interleukin-2. The presence of virus in the supernatant fluid was detected using reverse transcriptase and immunofluorescence assays. Supernatants with borderline activity were confirmed by infection of a continuous cell line.
D000386:AIDS-Related Complex / Q000382:microbiology*; D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D000328:Adult; D001315:Australia; D002555:Cerebrospinal Fluid / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D006678:HIV / Q000302:isolation & purification*; D006679:HIV Seropositivity / Q000382:microbiology*; D006801:Humans; D007962:Leukocytes / Q000382:microbiology; D008297:Male; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D012661:Semen / Q000382:microbiology
D012194:RNA-Directed DNA Polymerase