Three nuclear genes suppress a yeast mitochondrial splice defect when present in high copy number.
A gene bank of a yeast wild type DNA in the high copy number vector YEp13 was screened for recombinant plasmids which suppress the mitochondrial RNA splice defect exerted by mutant M1301, a -1 bp deletion in the first intron of the mitochondrial COB gene (bI1). A total of 17 recombinant plasmids with similar suppressor activity were found. Restriction mapping and cross-hybridization of the inserts revealed that these 17 plasmids contain three different inserts, all lacking any extended sequence homology. Each of the inserts, when present in high copy number, has a similar suppressor activity: high in the presence of mutation M1301 in bI1, a group II intron, and low but significant with the presence of few mutants in bI2 and bI3 of the COB gene, both of which are group I introns.
D000483:Alleles; D002467:Cell Nucleus / Q000378:metabolism*; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D005784:Gene Amplification; D005800:Genes, Fungal; D005822:Genetic Vectors; D008928:Mitochondria / Q000378:metabolism*; D009154:Mutation; D010957:Plasmids; D012313:RNA / Q000235:genetics*; D012326:RNA Splicing; D000077278:RNA, Mitochondrial; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013489:Suppression, Genetic
D000077278:RNA, Mitochondrial; D012313:RNA; D004262:DNA Restriction Enzymes