Sequence-dependence of the conformational changes induced by the 5-methyl cytosine in synthetic RNA oligomers.
The RNA hexamer containing a 5-methyl cytosine (m5C) r(CGUAm5CG) was studied by 1H and 31P NMR at 500 MHz and 121 MHz, respectively. In contrast to r(CGm5CGCG) which exhibits an atypic duplex structure [(1987) J. Am. Chem. Soc. 109, 2539-2541], r(CGUAm5CG) adopts a classical A-type conformation. This result demonstrates that the influence of the m5C on the conformation of RNA hexamers is sequence-dependent.
D044503:5-Methylcytosine; D003596:Cytosine / Q000031:analogs & derivatives*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides; D012313:RNA; D013329:Structure-Activity Relationship; D013816:Thermodynamics
D009843:Oligoribonucleotides; D012313:RNA; D044503:5-Methylcytosine; D003596:Cytosine