Regulated expression of human atrial natriuretic polypeptide gene in mouse L cells.
To investigate whether the human atrial natriuretic polypeptide (hANP) gene is responsive to glucocorticoid, we co-introduced the hANP gene (with SV40 enhancer) with HSV-tk gene into mouse tk- L cells. The transformants with hANP gene with SV40 enhancer expressed hANP specific RNAs. The administration of 1 microM dexamethasone reduced the expressed hANP specific RNAs, especially those that had a physiological initiation site. These results suggest that the hANP gene is really a glucocorticoid responsive gene and may be negatively regulated by glucocorticoid.
D000818:Animals; D009320:Atrial Natriuretic Factor / Q000235:genetics*; D003907:Dexamethasone / Q000494:pharmacology; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation / Q000187:drug effects; D005796:Genes / Q000187:drug effects; D006801:Humans; D007739:L Cells / Q000378:metabolism; D051379:Mice; D012313:RNA / Q000235:genetics / Q000378:metabolism; D013539:Simian virus 40 / Q000235:genetics; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D012313:RNA; D003907:Dexamethasone; D009320:Atrial Natriuretic Factor