Characterization of antisera to 2-hydroxyestradiol and 4-hydroxyestradiol using 6-(O-carboxymethyl)oxime- and 17-hemisuccinate-bovine serum albumin conjugates and radioimmunoassay.
For radioimmunoassay of the catechol estrogens, four hapten-bovine serum albumin (BSA) conjugates were prepared from 6-oxo-2-hydroxyestradiol 6-(O-carboxymethyl)oxime, 2-hydroxyestradiol 17-hemisuccinate, 6-oxo-4-hydroxyestradiol 6-(O-carboxymethyl)oxime and 4-hydroxyestradiol 17-hemisuccinate by coupling with BSA, employing the mixed anhydride method. The antisera elicited in rabbits by immunization with these antigens showed high affinity and specificity for 2-hydroxyestradiol or 4-hydroxyestradiol with cross-reactivities to a few structurally related estrogens. The specificity of antisera obtained is discussed in relation to the site of attachment of the hapten to BSA.
D000818:Animals; D000915:Antibody Affinity; D000918:Antibody Specificity; D000939:Epitopes; D004958:Estradiol / Q000031:analogs & derivatives* / Q000276:immunology; D002393:Estrogens, Catechol / Q000276:immunology*; D010091:Oximes; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods; D012710:Serum Albumin, Bovine / Q000276:immunology; D013329:Structure-Activity Relationship; D013386:Succinates
D000939:Epitopes; D002393:Estrogens, Catechol; D010091:Oximes; D013386:Succinates; D012710:Serum Albumin, Bovine; D004958:Estradiol