Polymer inaccessible volume changes during opening and closing of a voltage-dependent ionic channel.
Osmotic stress can be used to estimate the internal volume change during the opening and closing of a voltage gated ionic channel. Mitochondrial voltage-dependent anion channels, from rat liver and from Neurospora, reconstituted into planar lipid bilayers show a change of 2 to 4 X 10(4) A3 in internal volume, a large change inconsistent with a blocking or local gating model but supporting models with major closure of the channel space.
D000818:Animals; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D008051:Lipid Bilayers; D008564:Membrane Potentials; D008928:Mitochondria / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism; D008954:Models, Biological; D009492:Neurospora crassa / Q000378:metabolism; D011092:Polyethylene Glycols / Q000494:pharmacology*; D051381:Rats
D007473:Ion Channels; D008051:Lipid Bilayers; D011092:Polyethylene Glycols