Antibody against nuclear thyroid hormone receptors.
Rat liver nuclear thyroid hormone receptor was purified to 700-1600 pmol T3 binding capacity/mg protein by sequentially using hydroxylapatite column, ammonium sulfate precipitation, Sephadex G-150 gel filtration, DNA-cellulose column, DEAE-Sephadex A-50 column, and heparin-Sepharose column. Serum from a mouse immunized using this purified receptor preparation caused a shift of [125I]T3-receptor peak on glycerol density gradient sedimentation from 3.4 S to approximately 7 S. [125I]T3-receptor complex was immunoprecipitated using this serum and goat anti-mouse IgG. The serum showed reduced ability to immunoprecipitate the globular T3 binding fragment with Stokes radius of 22 A produced by trypsin digestion, a receptor fragment which has core histone and hormone binding but not DNA binding activity. These data indicate the production of anti-nuclear thyroid hormone receptor antibody which mainly recognized epitopes unrelated to hormone and core histone binding domain.
D000818:Animals; D000906:Antibodies / Q000276:immunology* / Q000302:isolation & purification; D000936:Antigen-Antibody Complex / Q000302:isolation & purification; D002467:Cell Nucleus / Q000276:immunology / Q000378:metabolism; D000939:Epitopes; D008099:Liver / Q000276:immunology / Q000378:metabolism; D051381:Rats; D011988:Receptors, Thyroid Hormone / Q000276:immunology* / Q000378:metabolism
D000906:Antibodies; D000936:Antigen-Antibody Complex; D000939:Epitopes; D011988:Receptors, Thyroid Hormone