Expansion and functional activity of Ly-1+ B cells upon transfer of peritoneal cells into allotype-congenic, newborn mice.
Transfer of peritoneal cells from adult donors into newborn, allotype-congenic mice led to colonization of the recipient mice by donor-derived B lymphocytes expressing the Ly-1 surface marker (Ly-1 B cells). These cells not only persisted in the recipient mice for at least 5 months, but also increased in number. In contrast, bone marrow-derived stem cells did not or scarcely give rise to B cells after intraperitoneal injection into congenic newborn recipients under the same experimental conditions. Approximately half of the IgM in the serum of peritoneal cell-recipients was produced by donor-derived Ly-1 B cells, suggesting that high levels of serum IgM in a normal mouse are produced by this B cell subpopulation. The transferred Ly-1 B cells were able to respond in a normal fashion to alpha (1----3)dextran, but they did not participate in thymus-dependent and -independent (TI II) immune responses to the hapten (4-hydroxy-3-nitrophenyl)acetyl (NP). In neither the immune response to alpha (1----3)dextran nor that to NP were we able to detect an influence of the transferred Ly-1 B cells on the selection of the idiotypic repertoire in the recipient mice.
D000818:Animals; D000831:Animals, Newborn / Q000276:immunology; D000950:Antigens, Ly / Q000032:analysis*; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000637:transplantation; D002455:Cell Division; D003911:Dextrans / Q000494:pharmacology; D007075:Immunoglobulin M / Q000096:biosynthesis; D007146:Immunoglobulin lambda-Chains / Q000096:biosynthesis; D051379:Mice; D009596:Nitrophenols / Q000276:immunology; D010529:Peritoneal Cavity / Q000166:cytology; D010648:Phenylacetates; D013154:Spleen / Q000166:cytology
D000950:Antigens, Ly; D003911:Dextrans; D007075:Immunoglobulin M; D007146:Immunoglobulin lambda-Chains; D009596:Nitrophenols; D010648:Phenylacetates; C026242:4-hydroxy-5-nitrophenyl acetic acid