Cord blood basophil releasability: evaluation of histamine release and leukotriene C4 generation.
This study was performed to evaluate mediator (histamine and leukotriene C4) release from cord blood and adult blood basophils, challenged with IgE-independent (calcium ionophore A23187) and IgE-mediated (anti-IgE) stimuli. IgE-independent mediator release was similar in adult blood and umbilical blood basophils. Conversely, the anti-IgE-induced histamine and immunoreactive leukotriene C4 (iLTC4) release was significantly reduced in cord blood basophils. Passive sensitization with an IgE-rich serum was followed by a significant increase in the number of eluted IgE molecules from cord blood basophils and by an increase in IgE-mediated histamine release. iLTC4 production was not affected by passive sensitization of umbilical blood basophils. The IgE-dependent mediator release from cord blood basophils was not correlated with the number of cell-bound IgE. In addition, histamine secretion and leukotriene C4 production from cord blood basophils seem to be independent events.
D000888:Antibodies, Anti-Idiotypic / Q000378:metabolism; D001491:Basophils / Q000378:metabolism*; D005312:Fetal Blood / Q000166:cytology*; D006632:Histamine / Q000097:blood; D006636:Histamine Release; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis / Q000276:immunology; D013189:SRS-A / Q000096:biosynthesis*
D000888:Antibodies, Anti-Idiotypic; D013189:SRS-A; D007073:Immunoglobulin E; D006632:Histamine