title: Adsorption to Al(OH), gel of Escherichia coli is correlated with O and K antigens and with type of extraintestinal infection.
abstract: Living suspensions of 89 Escherichia coli strains were tested for adsorption to Al(OH)3 gel in the presence of phosphate ions. On the basis of AC50 (phosphate molarity inhibiting 50% adsorption of the strain examined), E. coli strains could be classified into two main groups. Forty-three strains belonged to group 1 (AC50, 0.01-0.04), and 42 of them fell into serogroups O1, O2, O5, O7, O18ac, O83 or were spontaneously agglutinable. One strain in group 1 was exceptional as it had antigen O4. Of these 43 strains 33 had K antigen K1. Serogroup distribution of 46 group 2 strains (AC50, 0.001-0.009) was O2, O4, O6, O18ac, O75 and O78; 20 out of 46 possessed antigen K5. No correlation existed between H antigens or haemagglutinating capacity and AC50 of the strains. A close correlation was shown between AC50 pattern and the two main pathogenecity groups (i.e. "newborns' meningitis" and "sepsis and organotropic diseases") on one hand and between AC50 pattern and O, K serotypes on the other. The findings indicate that these E. coli strains with identical markers had a clonal connection.
mesh_terms: D000327:Adsorption; D000536:Aluminum Hydroxide / Q000378:metabolism*; D000942:Antigens, Bacterial / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D001422:Bacterial Adhesion; D004926:Escherichia coli / Q000145:classification* / Q000276:immunology / Q000472:pathogenicity / Q000502:physiology; D004927:Escherichia coli Infections / Q000382:microbiology*; D010861:Fimbriae, Bacterial / Q000648:ultrastructure; D006384:Hemagglutination; D006801:Humans; D008358:Mannose; D019081:O Antigens; D012703:Serotyping
chemical_list: D000942:Antigens, Bacterial; D000954:Antigens, Surface; C030659:K antigens; D019081:O Antigens; D000536:Aluminum Hydroxide; D008358:Mannose