cDNA cloning with a retrovirus expression vector: generation of a pp60c-src cDNA clone.
We used a murine retroviral expression vector, containing a genomic clone of the chicken c-src gene, a bacterial origin of replication, and a selectable marker, to remove 10 introns from the c-src gene. All 10 introns were removed accurately, and no mutations were introduced. The processed gene encoded a functional pp60c-src protein tyrosine kinase.
D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000235:genetics*; D004274:DNA, Recombinant; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D010750:Phosphoproteins / Q000235:genetics; D010766:Phosphorylation; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016392:Proto-Oncogene Proteins pp60(c-src); D012190:Retroviridae / Q000235:genetics*
D004274:DNA, Recombinant; D010750:Phosphoproteins; D011518:Proto-Oncogene Proteins; D004247:DNA; D016392:Proto-Oncogene Proteins pp60(c-src)