Effect of alpha-amanitin on liver non-histone chromatin proteins of starved hens.
A diminution of RNA content in hen liver nuclei was observed after either prolonged starvation or short-term exposure to alpha-amanitin. Using polyacrylamide gel electrophoresis, it has been revealed a limited number of altered polypeptide bands in the gel patterns of 0.35 M NaCl- and 5 M urea-soluble non-histone proteins from liver chromatin of starved or alpha-amanitin-treated birds. The low-molecular-weight polypeptides were found to increase in the protein fractions from liver chromatin of alpha-amanitin-injected hens. Only two protein bands (48 and 79 kDa) in the gel patterns of 5 M urea-soluble chromatin fraction altered in similar manner both in starved and alpha-amanitin-treated animals. The amount of the 48-kDa protein decreased and that of the 79-kDa protein increased under these conditions. alpha-Amanitin seems to affect differently the non-histone chromatin proteins from starved and fed animals. The level of the 48-kDa urea-soluble protein was lower and that of the 64-kDa protein was higher in liver chromatin of starved animals receiving alpha-amanitin in comparison with the corresponding proteins from fed animals treated with this drug.
D000546:Amanitins / Q000494:pharmacology*; D000818:Animals; D002645:Chickens; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis*; D005260:Female; D006657:Histones / Q000032:analysis; D008099:Liver / Q000032:analysis*; D012313:RNA / Q000032:analysis; D013217:Starvation / Q000378:metabolism*
D000546:Amanitins; D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D012313:RNA