title: Ionic channels in mouse astrocytes in culture.
abstract: We observed Na, K, and Cl voltage-dependent currents in a patch-clamp study of mouse brain astrocytes. In whole-cell recordings, depolarizations activated inward currents that were identified as Na currents since they were blocked by TTX, although complete block required high concentrations (greater than 1 microM). The corresponding single-channel Na currents were observed in outside-out patches. The channels were opened by a depolarizing pulse applied from a holding potential identical to the resting potential (-70 to -80 mV). Therefore, they may be considered functional Na channels. After addition of veratridine and an alpha-scorpion toxin, the decay of Na currents in whole-cell recordings was slower than observed under control conditions. At the single-channel level, the channels appeared to open in bursts. Depolarization did not increase the duration of the bursts, but inside each burst, increased the time spent in the open state. The K currents observed in the whole-cell recording mode were separated into inactivating and noninactivating currents. The inactivating current resembled the A current in its kinetics, its insensitivity to tetraethylammonium, and its sensitivity to 4-aminopyridine. At the single-channel level, at least 3 classes of K channels were observed at steady depolarized potentials. They resembled the K channels found in chromaffin cells by Marty and Neher (1985). Large conductance channels (385 pS) activated around 0 mV were identified as Cl channels.
mesh_terms: D015761:4-Aminopyridine; D000631:Aminopyridines / Q000494:pharmacology; D000818:Animals; D001253:Astrocytes / Q000032:analysis / Q000502:physiology*; D002478:Cells, Cultured; D002712:Chlorides / Q000378:metabolism*; D007473:Ion Channels / Q000032:analysis / Q000187:drug effects / Q000502:physiology*; D008564:Membrane Potentials; D051379:Mice; D011188:Potassium / Q000378:metabolism*; D012604:Scorpion Venoms / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism*; D013779:Tetrodotoxin / Q000494:pharmacology; D014701:Veratridine / Q000494:pharmacology
chemical_list: D000631:Aminopyridines; D002712:Chlorides; D007473:Ion Channels; D012604:Scorpion Venoms; D013779:Tetrodotoxin; D014701:Veratridine; D012964:Sodium; D015761:4-Aminopyridine; D011188:Potassium