Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples.
D000818:Animals; D002460:Cell Line; D002725:Chloroform; D019791:Guanidine; D006146:Guanidines; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008321:Mammary Glands, Animal / Q000032:analysis; D019800:Phenol; D010636:Phenols; D012313:RNA / Q000302:isolation & purification*; D051381:Rats; D012996:Solutions
D006146:Guanidines; D010636:Phenols; D012996:Solutions; D019800:Phenol; D012313:RNA; D002725:Chloroform; D019791:Guanidine