Antioxidants increase the cryoresistance of GM-CFC.
The influence of antioxidative drugs on the cryopreservation of human bone marrow cells was studied. The viability of bone marrow cells was tested after freezing and thawing from--196 degrees C by the growth of GM-CFC in agar culture. The results suggest the ability of antioxidants to protect stem cells against damage caused by freezing and thawing. Addition of tocopherol or penicillin to a cryoprotective medium increases the number of surviving GM-CFC. From the colony-to-cluster ratio can be concluded that antioxidants especially protect more immature haematopoietic (colony forming) cells.
D000975:Antioxidants / Q000494:pharmacology*; D001853:Bone Marrow / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects*; D003114:Colony-Forming Units Assay; D005615:Freezing; D006801:Humans; D014021:Tissue Preservation / Q000379:methods*
D000975:Antioxidants