Sequence-selective binding of phleomycin to DNA.
The binding of phleomycin and bleomycin to DNA has been investigated by studying their effects on cleavage by DNAase I and micrococcal nuclease. In the presence of cobalt, cleavage of DNA by the antibiotics is suppressed, yet they still provide protection from nuclease attack in regions surrounding the drug cleavage sites. We conclude that cleavage by phleomycin occurs at bonds around which the antibiotic is already selectively bound.
D001345:Autoradiography; D001483:Base Sequence; D001665:Binding Sites; D001761:Bleomycin / Q000378:metabolism*; D003035:Cobalt; D004247:DNA / Q000378:metabolism*; D003850:Deoxyribonuclease I; D008836:Micrococcal Nuclease; D010692:Phleomycins / Q000378:metabolism*
D010692:Phleomycins; C046585:phleomycin E; D001761:Bleomycin; D003035:Cobalt; D004247:DNA; D003850:Deoxyribonuclease I; D008836:Micrococcal Nuclease