Induction of in vitro antigen-specific antibody production against NIP-KLH in nonadherent cells derived from human peripheral blood mononuclear cells.
A primary in vitro antibody response to 4-hydroxy-3-iodo-5-nitrophenylacetyl keyhole limpet hemocyanin (NIP-KLH) by plastic dish nonadherent human peripheral blood mononuclear cells (PBMC) was examined. Nonadherent cells of most donors produced an anti-NIP antibody when they were cultured with the antigen at a cell density of 2.5 X 10(6)/ml in the presence of Staphylococcus aureus Cowan I (SAC) at a concentration of 0.003% for 5 days, washed and further cultured in the absence of the antigen and SAC for an additional 5 or 6 days. Nonadherent cells of 'low-responders' responded to NIP-KLH plus SAC and produced anti-NIP antibody when they were cultured with not only NIP-KLH plus SAC but also with the culture supernatant obtained on phytohemagglutinin (PHA) stimulation of a mixture of PBMC from 2 donors. Furthermore, it was found that the PHA supernatants could be very effectively substituted for by human recombinant interleukin-2.
D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology; D002448:Cell Adhesion; D002478:Cells, Cultured; D000939:Epitopes; D006433:Hemocyanins / Q000276:immunology*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000166:cytology*; D009592:Nitrohydroxyiodophenylacetate / Q000276:immunology*; D009596:Nitrophenols / Q000276:immunology*
D000939:Epitopes; D007376:Interleukin-2; D009596:Nitrophenols; D009592:Nitrohydroxyiodophenylacetate; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin