Use of a 1-hydroxybenzotriazole activated phosphorylating reagent towards the synthesis of short RNA fragments in solution.
Evidence will be presented to show that the reagents 3a-c (X = O) obtained by the reaction of 2-chlorophenylphosphorodichloridate with 1-hydroxybenzotriazole or 1-hydroxy-6-trifluoromethyl (6-nitro) benzotriazoles, respectively, do not give, under normal coupling conditions, phosphorylation of the lactam functions in uracil or guanine. Reagent 3b (X = O) proved to be very convenient for the in situ formation of 3'-5'-phosphotriester linkage. The latter will be illustrated by the synthesis of several RNA fragments.
D001483:Base Sequence; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D010766:Phosphorylation; D012313:RNA / Q000138:chemical synthesis*; D012997:Solvents; D014230:Triazoles
D007202:Indicators and Reagents; D009843:Oligoribonucleotides; D012997:Solvents; D014230:Triazoles; D012313:RNA; C011852:1-hydroxybenzotriazole