[Assessment of the specific activity of commercial allergen preparations made of house dust in the bacteriosorption reaction: a comparison with the results of the radioallergosorbent inhibition test].
The activity of the commercial batches of house-dust (HD) allergens was compared in the inhibition of the radioallergosorbent test (RAST) and in the direct bacteriosorbent test (BST), detecting IgG to the antigens by adsorption on the complexes of whole staphylococcal cells containing protein A. BST was made with rabbit antiserum to HD allergen. This antiserum inhibited RAST by 76% and, therefore, contained antibodies to most of the allergenic determinants of HD. At the same time, no significant correlation between the activity of 15 batches of HD allergen was revealed in RAST inhibition and in BST with the above antiserum. Nevertheless, the exhaustion of the antiserum with a batch of HD allergen showing low activity in RAST inhibition, but high activity in BST made it possible to obtain BST results significantly correlating with the data resulting from RAST inhibition in two series of experiments.
D000485:Allergens / Q000032:analysis / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000032:analysis; D004391:Dust / Q000032:analysis; D000939:Epitopes / Q000032:analysis; D006801:Humans; D007163:Immunosorbent Techniques; D011817:Rabbits; D011852:Radioallergosorbent Test / Q000379:methods; D012882:Skin Tests; D013205:Staphylococcal Protein A / Q000276:immunology
D000485:Allergens; D000906:Antibodies; D004391:Dust; D000939:Epitopes; D013205:Staphylococcal Protein A
Otsenka spetsificheskoĭ aktivnosti kommercheskikh preparatov allergenov iz domashneĭ pyli v reaktsii bakteriosorbtsii: sravnenie s rezul'tatami tormozheniia radioallergosorbentnogo testa.