Production in vitro of thyroglobulin autoantibody by obese strain (OS) chickens.
Thyroglobulin autoantibody (Tg-AAb) can be spontaneously produced in vitro with thyroid infiltrating lymphocytes (TIL) collected from Obese strain chickens 3.5 and 4 weeks old. Attempts to enhance Tg-AAb synthesis with two known polyclonal stimulators of immunoglobulin synthesis in chickens, Staphylococcus aureus Cowan strain 1 and dextran sulphate, failed to increase Tg-AAb production in vitro. Spleen cells and peripheral blood lymphocytes obtained from the same chickens as the TIL and older chickens known to produce moderate to high levels of Tg-AAb in vivo did not produce autoantibody either spontaneously or in the presence of polyclonal Ig stimulators with one exception. With this single, exceptional chicken we obtained a small amount of Tg-AAb produced in vitro with spleen cells. This suggests that in the OS chicken TIL, and to a much lesser extent, the spleen, contribute to the total Tg-AAb produced in this model of autoimmune thyroiditis.
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001323:Autoantibodies / Q000096:biosynthesis*; D002478:Cells, Cultured; D002645:Chickens; D003208:Concanavalin A / Q000494:pharmacology; D016264:Dextran Sulfate; D003911:Dextrans / Q000494:pharmacology; D005260:Female; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D013154:Spleen / Q000276:immunology; D013211:Staphylococcus aureus / Q000276:immunology; D013954:Thyroglobulin / Q000276:immunology*
D000942:Antigens, Bacterial; D001323:Autoantibodies; D003911:Dextrans; D003208:Concanavalin A; D013954:Thyroglobulin; D016264:Dextran Sulfate