title: The origin of ciliated cell cysts of the anterior pituitary. An experimental study in the rat.
abstract: Rats were decapitated and the heads were stored at 4 degrees C for 24 h. The anterior pituitaries were then removed and incubated for 5 days. On the 1st, 3rd and 5th days of incubation, explants were studied by electron microscopy and immunohistochemistry using antiserum against S-100 protein. In the explant many granulated cells underwent necrosis; folliculo-stellate (FS) cells formed many cyst-like structures (CLSs) and became squamous epithelioid cells (CLS-forming cells). After incubation for 5 days the explants were isotransplanted under the renal capsules of male rats in order to observe morphological changes in the CLSs. Immediately after transplantation, the CLSs were encircled by a basement membrane, but from the 8th to 14th day, ciliation occurred in CLS-forming cells and "ciliated cell cysts" were formed. The ciliated cells were immunostained with antiserum against S-100 protein. The present study suggests that FS cells are related to CLS formation and have the potential to trans-differentiate to ciliated cells.
mesh_terms: D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000378:metabolism / Q000473:pathology*; D002923:Cilia / Q000378:metabolism / Q000473:pathology; D007106:Immune Sera / Q000494:pharmacology; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D009336:Necrosis; D009368:Neoplasm Transplantation; D009924:Organ Culture Techniques; D010903:Pituitary Gland, Anterior / Q000378:metabolism / Q000473:pathology* / Q000637:transplantation; D010911:Pituitary Neoplasms / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D011919:Rats, Inbred Strains; D009418:S100 Proteins / Q000276:immunology; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors
chemical_list: D007106:Immune Sera; D009418:S100 Proteins