Selection and characterization of monoclonal antibodies against a major allergen in Dermatophagoides pteronyssinus. Species-specific and common epitopes in three dermatophagoides species.
Hybridomas were produced after immunization with Der p I (Ag 42, P1). Specific anti-Der p I activity was detected in a reverse ELISA using immobilized anti-mouse Ig and highly purified Der p I labelled with horseradish peroxidase. Stable subclones from 6 parental hybridomas were obtained. The species specificities of the monoclonal antibodies (MABs) were classified: by an overlayer technique analogous to the CRIE technique, and by two competitive ELISA techniques. One MAB recognized an epitope specific for Dermatophagoides pteronyssinus. This MAB was successfully employed as a coating reagent in a species-specific ELISA for Der p I in house dust. The remaining antibodies reacted with epitopes shared to varying degrees by either of two other Dermatophagoides species. MABs recognizing epitopes common to all 3 species were not observed. Mutual cross-inhibition studies revealed four topographically distinct antibody binding sites on Der p I.
D000485:Allergens / Q000032:analysis / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D039741:Antigens, Dermatophagoides; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis* / Q000276:immunology; D005260:Female; D006825:Hybridomas / Q000378:metabolism; D007123:Immunoelectrophoresis, Two-Dimensional; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008925:Mites / Q000276:immunology*; D013045:Species Specificity
D000485:Allergens; D000911:Antibodies, Monoclonal; D039741:Antigens, Dermatophagoides; D000939:Epitopes