RNA end-labeling and RNA ligase activities can produce a circular rRNA in whole cell extracts from trypanosomes.
We have found two enzymatic activities in whole cell extracts from Trypanosoma brucei; an end-labeling reaction involving a single uridine at the 3' end of ribosomal RNAs (rRNAs) and an RNA ligase activity joining 5' monophosphates to 3' hydroxyl groups. The RNA ligase acts upon one of the small rRNAs (180 nucleotides) from the trypanosome ribosomal repeat unit, forming a circular RNA. The specific circularization of this small rRNA is probably dependent on the secondary structure of the molecule and is not detectable in vivo.
D000818:Animals; D007553:Isotope Labeling / Q000379:methods; D009690:Nucleic Acid Conformation; D011118:Polynucleotide Ligases / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis*; D011130:RNA Ligase (ATP) / Q000378:metabolism*; D012316:RNA Nucleotidyltransferases / Q000378:metabolism*; D000079962:RNA, Circular; D012335:RNA, Ribosomal / Q000378:metabolism*; D014346:Trypanosoma brucei brucei / Q000201:enzymology* / Q000235:genetics; D014544:Uridine Triphosphate / Q000378:metabolism
D000079962:RNA, Circular; D012335:RNA, Ribosomal; D012313:RNA; D012316:RNA Nucleotidyltransferases; C031687:UTP-RNA uridylyltransferase; D011118:Polynucleotide Ligases; D011130:RNA Ligase (ATP); D014544:Uridine Triphosphate