Isolation and characterization of a specific antibody population against human fibrinogen.
A specific antibody population against human fibrinogen was isolated from a rabbit antiserum by affinity chromatography on fibrinogen-bound Sepharose gel. Using a sensitive competitive radioimmunoassay, the antibody population was found to recognize epitopes on native fibrinogen but crossreacted minimally with fibrinogen fragment D and an early plasmin-degraded fibrinogen A alpha-chain product, but not at all with fragment E or fibrinopeptides A and B. Fibrin monomers shared part of these epitopes. The antibody population crossreacted to a small extent with bovine, horse and baboon fibrinogens and not at all with fibrinogens from sheep, rat, pig, goat, guinea pig, dog and rabbit.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000936:Antigen-Antibody Complex / Q000032:analysis; D002846:Chromatography, Affinity / Q000379:methods; D000939:Epitopes / Q000032:analysis; D005340:Fibrinogen / Q000276:immunology*; D006801:Humans; D007106:Immune Sera; D005779:Immunodiffusion; D007700:Kinetics; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay
D000906:Antibodies; D000936:Antigen-Antibody Complex; D000939:Epitopes; D007106:Immune Sera; D005340:Fibrinogen