Oxidation catalyzed by H+ ions improves the silver intensification of 3,3'-diaminobenzidine staining by strongly suppressing tissue argyrophilia.
We found that a 15-min treatment of tissue sections with 2 M/l H2O2 dissolved in 6 M/l sulphuric acid strongly suppresses the argyrophilia of tissue elements, thus facilitating selective silver intensification in histochemical procedures. When applied inserted between the 3,3'-diaminobenzidine reaction and physical development, this treatment allows the selective and sensitive visualization of very low levels of peroxidase activity.
D015100:3,3'-Diaminobenzidine; D001560:Benzidines; D006801:Humans; D010084:Oxidation-Reduction; D011522:Protons; D012834:Silver; D013194:Staining and Labeling / Q000379:methods*
D001560:Benzidines; D011522:Protons; D015100:3,3'-Diaminobenzidine; D012834:Silver