A simple and rapid in situ staining method for granulocyte-macrophage colonies in agar culture.
This report describes a very simple, quick and effective method for in situ staining of granulocyte-macrophage colonies in agar cultures and for classifying various colony types. The procedure takes only one minute to fix and a few minutes to stain; a few additional minutes are required for preparation of the permanent whole plate. In this process the Riu stain, a modified Romanowsky stain, is used. Besides the ease and rapidity of this procedure, the identification of colony types appears to be enhanced. Thus, the method seems to be very beneficial in routine observations of colony types.
D000362:Agar; D001399:Azure Stains; D002021:Buffers; D003114:Colony-Forming Units Assay; D004801:Eosine Yellowish-(YS); D006098:Granulocytes / Q000032:analysis*; D006801:Humans; D008264:Macrophages / Q000032:analysis*; D000432:Methanol; D008751:Methylene Blue; D013194:Staining and Labeling / Q000379:methods*
D001399:Azure Stains; D002021:Buffers; D000362:Agar; D008751:Methylene Blue; D004801:Eosine Yellowish-(YS); D000432:Methanol