title: Combined GABA-immunocytochemistry and TMB-HRP histochemistry of pretectal nuclei projecting to the inferior olive in rats, cats and monkeys.
abstract: Cells in the pretectal nucleus of the optic tract (NOT) of rats, cats and monkeys were retrogradely labeled with horseradish peroxidase (HRP) stereotaxically injected into the inferior olive (IO). A procedure for stabilizing the tetramethylbenzidine (TMB)-HRP reaction product was used to visualize combined TMB-HRP and immunohistochemically localized gamma-aminobutyric acid (GABA) in the same sections. Positive GABAergic reaction product was found to be restricted to smaller-size intrinsic neurons. Larger NOT cells projecting to the IO were consistently free of GABA reaction product and, in addition, appeared to be contacted by relatively few GABAergic terminals.
mesh_terms: D000818:Animals; D002415:Cats; D008253:Macaca mulatta; D008636:Mesencephalon / Q000378:metabolism*; D009434:Neural Pathways / Q000378:metabolism; D009847:Olivary Nucleus / Q000378:metabolism*; D051381:Rats; D013194:Staining and Labeling; D014795:Visual Pathways / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D005680:gamma-Aminobutyric Acid