Intraduodenal instillation of acid and hyperosmolal solution suppresses pentagastrin-stimulated acid secretion but not histamine mobilization in the rat stomach.
In chronic gastric fistula rats provided with a duodenal loop anastomosed to the jejunum (Roux-en-Y), maximal stimulation of acid secretion by continuous intravenous infusion of pentagastrin produced a 15-fold increase of gastric histidine decarboxylase activity. Intraduodenal instillation of 0.20 M HCl or 1200 mOsm X kg-1 solution of polyethylene glycol (PEG) inhibited the maximal acid response to pentagastrin by 61% and hyperosmolal (1200 mOsm X kg-1 of PEG solution) 0.20 M HCl by 79% but did not suppress the pentagastrin-induced increase in enzyme activity. Hence, instillation of HCl and/or hyperosmolal PEG solution in the duodenum depresses the responsiveness of the parietal cells to pentagastrin without interfering with pentagastrin-induced activation of gastric histamine formation.
D000713:Anastomosis, Roux-en-Y; D000818:Animals; D004386:Duodenum / Q000187:drug effects; D005744:Gastric Acid / Q000378:metabolism*; D006636:Histamine Release / Q000187:drug effects*; D006640:Histidine Decarboxylase / Q000493:pharmacokinetics; D006851:Hydrochloric Acid / Q000008:administration & dosage*; D007583:Jejunum / Q000601:surgery; D008297:Male; D009994:Osmolar Concentration; D010418:Pentagastrin / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000008:administration & dosage*; D051381:Rats; D011919:Rats, Inbred Strains; D012965:Sodium Chloride / Q000008:administration & dosage
D011092:Polyethylene Glycols; D012965:Sodium Chloride; D006640:Histidine Decarboxylase; D010418:Pentagastrin; D006851:Hydrochloric Acid