The effect of diazepam and Ro 15-1788 on extracellular ascorbic acid, DOPAC and 5-HIAA in the striatum of anaesthetized and conscious freely moving rats, as measured by differential pulse voltammetry.
The effects of diazepam (10 mg/kg i.p.) and the central benzodiazepine receptor antagonist, Ro 15-1788 (30 mg/kg i.p.), on extracellular ascorbate, 3,4-dihydroxyphenylacetic acid (DOPAC) and 5-hydroxyindoleacetic acid (5-HIAA) were examined using differential pulse voltammetry in anaesthetized and freely moving rats. In anaesthetized animals, diazepam did not significantly alter the heights of peak 1 (ascorbate) or peak 3 (5-HIAA), but significantly reduced that of peak 2 (DOPAC). In freely moving rats, diazepam greatly reduced the heights of all 3 peaks. Ro 15-1788, injected 2 h after diazepam, reversed the effect of diazepam on peak 3, but not on peaks 1 and 2.
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D003975:Diazepam / Q000494:pharmacology*; D004563:Electrochemistry; D005110:Extracellular Space / Q000378:metabolism; D005442:Flumazenil / Q000494:pharmacology*; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D015102:3,4-Dihydroxyphenylacetic Acid; D005442:Flumazenil; D006897:Hydroxyindoleacetic Acid; D001205:Ascorbic Acid; D003975:Diazepam