title: Cryoprecipitation. In vitro studies on fibronectin as an independent cryoprecipitagogue.
abstract: Fifteen patients affected by essential mixed cryoglobulinemia or secondary cryoglobulins were studied. Fibronectin was detected in the majority of essential mixed cryoglobulinemias, but in a minority of secondary cryoglobulins. In vitro studies gave strong evidence of an interaction between fibronectin and heat-aggregated gammaglobulins. The effects of fibronectin on cryoprecipitation of heat-aggregated gammaglobulins seem to support the independent role of the glycoprotein in the cryoformation mechanism, although its presence does not appear essential for cryoglobulin appearance.
mesh_terms: D011232:Chemical Precipitation; D003080:Cold Temperature; D003166:Complement Activating Enzymes / Q000378:metabolism; D015922:Complement C1q; D003449:Cryoglobulinemia / Q000097:blood; D003450:Cryoglobulins / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D005353:Fibronectins / Q000302:isolation & purification*; D006358:Hot Temperature; D006801:Humans; D007122:Immunoelectrophoresis; D011092:Polyethylene Glycols / Q000494:pharmacology; D011485:Protein Binding; D012217:Rheumatoid Factor / Q000378:metabolism; D005719:gamma-Globulins / Q000378:metabolism
chemical_list: D003450:Cryoglobulins; D005353:Fibronectins; D005719:gamma-Globulins; D011092:Polyethylene Glycols; D015922:Complement C1q; D012217:Rheumatoid Factor; D003166:Complement Activating Enzymes