[The use of fractionation in a polyethylene glycol-dextran two-phase system for the testing and purification of restriction endonucleases].
A simple procedure was developed for testing and purification of restriction endonucleases Msp I, Pst I, Bam HI, Pvu I, Pvu II that includes biomass destruction, fractionation of cell-free extracts in the aqueous two-phase (polyethylene glycol-dextran) system and chromatography oh phosphocellulose. Optimal conditions for the fractionation of Msp I, Pst I, Bam HI, Pvu II, EcoR I, EcoR II, BspR I, Alu I were chosen. For separation of Pvu I and Pvu II gel filtration through biogel A-0.5 m was additionally introduced.
D005591:Chemical Fractionation; D004262:DNA Restriction Enzymes / Q000302:isolation & purification*; D003911:Dextrans; D011092:Polyethylene Glycols
D003911:Dextrans; D011092:Polyethylene Glycols; D004262:DNA Restriction Enzymes
Ispol'zovanie fraktsionirovaniia v dvukhfaznoĭ sisteme poliétilenglikol'-dekstran dlia testirovaniia i ochistki éndonukleaz restriktsii.