Formation of triple-helical nucleic acids studied by using antibodies specific for poly(A).poly(U).poly(U).
The formation of the triple helix of poly(A).poly(U).poly(U) was studied by using antibodies specific to poly(A).poly(U).poly(U). the 10-11 base chain length for oligo(A) and the 20-30 base chain length for oligo(U) may be the minimum sizes required to maintain a stable triple helix. Double-stranded poly(A).poly(U) which was the core of triple-stranded poly(A).poly(U).poly(U) could bind poly(U) and produce an analogue of poly(A).poly(U).poly(U) reactive with the antibodies even if the poly(A) or poly(U) was brominated or acetylated to the extent of 35-55%. However, brominated or acetylated poly(U) did not produce a stable triple helix with double-stranded poly(A).poly(U).
D000107:Acetylation; D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D001966:Bromine; D003168:Complement Fixation Tests; D007159:Immunologic Tests; D009690:Nucleic Acid Conformation; D011063:Poly A-U / Q000276:immunology; D011072:Poly U / Q000031:analogs & derivatives / Q000378:metabolism; D012313:RNA; D011863:Radioimmunoassay
D000906:Antibodies; D000941:Antigens; D011063:Poly A-U; D011072:Poly U; C031089:poly-5-bromouridylic acid; D012313:RNA; D001966:Bromine