title: Ultraviolet-induced suppressor T cells and factor(s) in murine contact photosensitivity. I. Biological and immunochemical characterization of factor(s) extracted from suppressor T cells.
abstract: Murine contact photosensitivity (CPS) to 3,3',4',5-tetrachlorosalicylanilide (TCSA) is a highly specific, T-cell-mediated delayed-type hypersensitivity (DTH). Preexposure of the photosensitizing site to low doses of ultraviolet B(UVB) rendered mice unresponsive to challenge reaction. This unresponsiveness was associated with the generation of antigen-specific, afferent limb-acting, Lyt-1+2-,L3T4+ suppressor T cells (Ts-cps) in the spleen, thymus, and lymph node. Cell-free extract(s) obtained by freezing and thawing of these cells contained T-cell-suppressor factor (TsF) that inhibited the development of the induction phase of the CPS response to TCSA in vivo in an antigen-specific fashion. The treatments of TsF both with immunoadsorbent columns and with reduction and alkylation showed that the factor bore photoantigen-binding site(s), was reactive with monoclonal anti-I-Jd, anti-I-E alpha but not anti-I-Ad, and behaved as a single-chain factor containing both photoantigen binding and I-J molecules. By gel chromatography the majority of the suppressive activity was eluted in the fractions corresponding to molecular weights of 60-80 and 100-200 kDa. Our present study demonstrated clearly that UVB-induced unresponsiveness in the DTH reaction was mediated by a soluble suppressive factor derived from T cells.
mesh_terms: D000478:Alkylation; D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D001665:Binding Sites; D003877:Dermatitis, Contact / Q000276:immunology*; D000939:Epitopes; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007108:Immune Tolerance / Q000528:radiation effects; D007111:Immunity, Cellular / Q000528:radiation effects; D051379:Mice; D008970:Molecular Weight; D010084:Oxidation-Reduction; D012458:Salicylanilides / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013329:Structure-Activity Relationship; D013491:Suppressor Factors, Immunologic / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology; D014466:Ultraviolet Rays
chemical_list: D000945:Antigens, Differentiation, T-Lymphocyte; D000939:Epitopes; D000949:Histocompatibility Antigens Class II; D012458:Salicylanilides; D013491:Suppressor Factors, Immunologic; C003390:3,3',4',5-tetrachlorosalicylanilide