title: Two-dimensional protein mapping by gold stain and immunoblotting.
abstract: A method is described for mapping individual polypeptides on a two-dimensional separation of a complex mixture of proteins. Polypeptides are identified by immunoblotting on nitrocellulose prestained for total protein by a colloidal gold stain which does not inhibit antibody binding. The same polypeptides can subsequently be identified on gold-stained blots run under identical conditions, without the need for immunoblotting.
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D006046:Gold; D006801:Humans; D007120:Immunochemistry; D008929:Mitochondria, Heart / Q000032:analysis; D009124:Muscle Proteins / Q000032:analysis; D010449:Peptide Mapping / Q000379:methods*; D013194:Staining and Labeling
chemical_list: D009124:Muscle Proteins; D006046:Gold