Hydroxyindole-O-methyltransferase in Y-79 human retinoblastoma cells: effect of cell attachment.
Hydroxyindole-O-methyltransferase (HIOMT), the enzyme in the final step of melatonin synthesis, is present in the Y-79 human retinoblastoma cell line. Using electroblot immunolabellings, a single band corresponding to HIOMT was observed. Immunofluorescence, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and quantification of enzyme activity all revealed dramatic increases in HIOMT in cells attached to substrate compared to cells in suspension culture.
D000121:Acetylserotonin O-Methyltransferase / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002460:Cell Line; D003600:Cytosol / Q000201:enzymology; D005134:Eye Neoplasms / Q000201:enzymology* / Q000473:pathology; D005455:Fluorescent Antibody Technique; D006801:Humans; D008970:Molecular Weight; D012160:Retina / Q000201:enzymology / Q000648:ultrastructure; D012175:Retinoblastoma / Q000201:enzymology* / Q000473:pathology; D013194:Staining and Labeling
D000121:Acetylserotonin O-Methyltransferase