[Enzymatic synthesis of polyprenyl phosphosugars in Salmonella serotype C1].
The cell envelopes of serogroup C1 Salmonella, viz. S. thompson and S. montevideo, catalyze the transfer of radiolabeled sugars from UDP-[14C]Glc and UDP-[14C]GlcNAc into the lipid-linked sugars. Using TLC and DEAE-cellulose chromatography, the radiolabeled products were identified as polyprenyl pyrophosphate N-acetylglucosamine (I), polyprenyl monophosphate N-acetylglucosamine and polyprenyl monophosphate glucose. The derivative (I) served as an acceptor for mannose transfer from GDP-Man with formation of Man1-2GlcNAc1PPPre. A similar reaction was observed after addition of synthetic GlcNAc1PPPre to the cell envelopes.
D000942:Antigens, Bacterial / Q000032:analysis*; D002850:Chromatography, Gel; D019081:O Antigens; D011135:Polysaccharides, Bacterial / Q000138:chemical synthesis*; D012475:Salmonella / Q000145:classification / Q000276:immunology*; D013403:Sugar Phosphates / Q000138:chemical synthesis*
D000942:Antigens, Bacterial; D019081:O Antigens; D011135:Polysaccharides, Bacterial; D013403:Sugar Phosphates
Fermentativnyĭ sintez poliprenilfosfosakharov s salmonellakh serogruppy C1.