Methylation of Marek's disease virus DNA in chicken T-lymphoblastoid cell lines.
Methylation of Marek's disease virus serotype 1 (MDV1) DNA in both productively and latently infected cells was examined by restriction endonuclease analyses with the isoschizomeric pair HpaII and MspI. The latent MDV1 DNA in T-lymphoblastoid cell lines established from chicken T-lymphomas was considerably methylated, whereas methylation of the virus DNA sequences was not detected in productively infected cells.
D044503:5-Methylcytosine; D000818:Animals; D002460:Cell Line; D002645:Chickens; D003596:Cytosine / Q000031:analogs & derivatives / Q000032:analysis; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis*; D019003:Deoxyribonuclease HpaII; D008381:Herpesvirus 2, Gallid / Q000235:genetics*; D008380:Marek Disease / Q000032:analysis; D008745:Methylation; D013601:T-Lymphocytes / Q000032:analysis*
D004273:DNA, Neoplasm; D004279:DNA, Viral; D044503:5-Methylcytosine; D003596:Cytosine; D004262:DNA Restriction Enzymes; D019003:Deoxyribonuclease HpaII