Retention of pinocytized solute by CHO cell lysosomes correlates with molecular weight.
We compared the exocytosis by Chinese hamster ovary (CHO) cells of a set of fluid-phase pinocytic tracers. The tracers were horseradish peroxidase (HRP), a glycoprotein of approximately 40 kDa, lucifer yellow (LuY), a 457 dalton, membrane-impermeant fluorescent dye, and glucose polymers ranging from sucrose through higher molecular weight, fluorescein isothiocyanate (FITC) dextrans. After a long term uptake (16-20 h), each of these tracers was localized to lysosomes. Exocytosis of the majority of the small molecule tracers, LuY and [14C] sucrose, was observed over a period of a few to several h. There was no significant exocytosis of 42 kDa FITC dextran or HRP during an 18-20 h chase, while lower molecular weight dextrans were exocytosed. After co-accumulation of LuY and HRP in lysosomes, only the low molecular weight marker was exocytosed. These observations suggest retention of endocytized solutes within lysosomes is dependent on molecular size and may be limited by the rate of diffusion of molecules into shuttle vesicles.
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003911:Dextrans / Q000378:metabolism; D005089:Exocytosis; D005260:Female; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives*; D005452:Fluoresceins / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000378:metabolism; D007546:Isoquinolines / Q000378:metabolism; D007700:Kinetics; D008247:Lysosomes / Q000378:metabolism*; D008970:Molecular Weight; D010053:Ovary; D010873:Pinocytosis; D013395:Sucrose / Q000378:metabolism
D003911:Dextrans; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006023:Glycoproteins; D007546:Isoquinolines; C015219:fluorescein isothiocyanate dextran; D013395:Sucrose; C017475:lucifer yellow; D006735:Horseradish Peroxidase; D016650:Fluorescein-5-isothiocyanate