Glucose metabolism via the Embden-Meyerhof pathway is not involved in ATP production during spore germination of bacillus megaterium QM B1551. A study with a mutant lacking hexokinase.
In order to investigate contributions by glucose metabolism via the Embden-Meyerhof pathway and that via the direct oxidation route to gluconate to initial ATP production during spore germination, respiratory activity and RNA synthesis were compared between the mutant lacking hexokinase and the parent spores of Bacillus megaterium QM B1551. We found that time courses of those metabolic events were almost identical between those spores, thus clearly indicating that NADH formed by a spore-specific enzyme glucose dehydrogenase (EC 1.1.1.47) is solely responsible for aerobic production of ATP at this stage.
D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000332:Aerobiosis; D001410:Bacillus megaterium / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D002237:Carbohydrate Dehydrogenases / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D042843:Glucose 1-Dehydrogenase; D005948:Glucose Dehydrogenases / Q000378:metabolism*; D006593:Hexokinase / Q000378:metabolism*; D009154:Mutation; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D012329:RNA, Bacterial / Q000096:biosynthesis; D013171:Spores, Bacterial
D012329:RNA, Bacterial; D009243:NAD; D000255:Adenosine Triphosphate; D002237:Carbohydrate Dehydrogenases; D005948:Glucose Dehydrogenases; D042843:Glucose 1-Dehydrogenase; D006593:Hexokinase; D005947:Glucose