Acidic heparin binding growth factor transiently activates adenylate cyclase activity in human adult arterial smooth muscle cells.
Acidic heparin binding growth factor (HBGF-1) is a potent mitogen for human adult arterial smooth muscle cells (SMC) in culture. Exposure of quiescent SMC to HBGF-1 resulted in a rapid and transient stimulation of the adenylate cyclase activity. Maximal stimulation (2-fold) was observed at 60 sec after growth factor exposure and there was a concurrent elevation of intracellular cAMP levels. GTP analogue was required indicating that the activation of this enzyme is mediated through G-proteins. The results suggest that part of the signal transduction pathway of HBGF-1 might be mediated by cAMP dependent protein kinase.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000328:Adult; D000242:Cyclic AMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D016220:Fibroblast Growth Factor 1; D006133:Growth Substances / Q000494:pharmacology*; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000201:enzymology*; D013806:Theophylline / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism
D006133:Growth Substances; D016220:Fibroblast Growth Factor 1; D006165:Guanylyl Imidodiphosphate; D006493:Heparin; D013806:Theophylline; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D013936:Thymidine