Synthesis and turnover rates of four rat liver mitochondrial RNA species.
The synthesis and turnover rates of the two 12 S and 16 S mt rRNAs and of the mt mRNAs for subunits I and III of cytochrome oxidase have been determined by measuring the kinetics of incorporation of [3H]uridine in the mtRNA of rat hepatocytes. All the RNA species examined have approximately the same turnover (t1/2 approximately 100 min) and therefore the rate of synthesis, which is about 10-times higher for the rRNAs, seems to be the factor responsible for the different mt rRNA and mRNA steady-state levels.
D000818:Animals; D003576:Electron Transport Complex IV / Q000235:genetics; D006207:Half-Life; D007700:Kinetics; D008297:Male; D008433:Mathematics; D008930:Mitochondria, Liver / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism; D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal / Q000096:biosynthesis / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014158:Transcription, Genetic; D014529:Uridine / Q000378:metabolism
D012333:RNA, Messenger; D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal; D012313:RNA; D003576:Electron Transport Complex IV; D014529:Uridine