[Method of staining for lysosomal cationic proteins in histological paraffin sections of biopsies].
A method is suggested for a histochemical and immunofluorescent detection of lysosome cation proteins in the histologic paraffin sections of biopsies. The method is based on the staining of histologic sections obtained from the material fixed in glutaraldehyde buffered with an alcohol solution of a fast green (pH 8.1-8.2) and on the detection myeloperoxidase and lactoferrine by means immune serum by Coons's method. 0.25% solution of azur A (B, C) is used for staining the cell nuclei and viable bacteria.
D001706:Biopsy; D004386:Duodenum / Q000378:metabolism / Q000473:pathology; D005455:Fluorescent Antibody Technique; D005753:Gastric Mucosa / Q000378:metabolism / Q000473:pathology; D006651:Histocytochemistry; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D008247:Lysosomes / Q000378:metabolism*; D010232:Paraffin; D011506:Proteins / Q000378:metabolism*; D013194:Staining and Labeling / Q000379:methods*
D011506:Proteins; D010232:Paraffin
Metodika okraski lizosomnykh kationnykh belkov v gistologicheskikh parafinovykh srezakh bioptatov.