Inhibition of topoisomerase I by antibodies in sera from scleroderma patients.
Purified type I topoisomerase from calf thymus as well as nuclear and cytoplasmic extracts from EGF-stimulated human and mouse fibroblasts in cell culture efficiently convert supercoiled plasmid DNA to the relaxed form. The purified IgG fraction from the sera of Japanese patients with the rheumatic disease scleroderma were shown to inhibit this relaxation activity. Thus, these patients likely produce autoantibodies to topoisomerase I. In addition, the human, bovine and murine enzymes share antigenic determinants recognized by the antisera.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D002417:Cattle; D002467:Cell Nucleus / Q000201:enzymology; D002478:Cells, Cultured; D004264:DNA Topoisomerases, Type I / Q000276:immunology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D000939:Epitopes / Q000032:analysis; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification; D007700:Kinetics; D051379:Mice; D012595:Scleroderma, Systemic / Q000276:immunology*; D013950:Thymus Gland / Q000201:enzymology; D059004:Topoisomerase I Inhibitors
D000906:Antibodies; D000939:Epitopes; D007074:Immunoglobulin G; D059004:Topoisomerase I Inhibitors; D004815:Epidermal Growth Factor; D004264:DNA Topoisomerases, Type I