IgM antibodies recognizing carbohydrate epitopes shared between schistosomula and miracidia of Schistosoma mansoni that block in vitro killing.
A series of monoclonal antibodies (mAb) was raised in mice against Schistosoma mansoni, which recognized a carbohydrate determinant on a major Mr greater than 200,000 schistosomulum surface antigen. These mAb cross-reacted with the surface of cercariae and miracidia and with schistosomula of S. haematobium and S. bovis. Other mAb were generated that only recognized a Mr 20,000 schistosomulum surface antigen; they did not cross-react with eggs or miracidia and were species specific. The anti-Mr 20,000 mAb of the IgG1 isotype exhibited high levels of complement-dependent cytotoxicity to schistosomula in vitro. IgM mAb that recognized carbohydrate epitopes of the Mr greater than 200,000 surface antigen blocked the lethal activity of the anti-Mr 20,000 mAb. The IgM anti-Mr greater than 200,000 mAb also reduced complement-dependent cytotoxicity of serum from mice vaccinated with irradiated cercariae.
D000818:Animals; D000910:Antibodies, Heterophile / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology*; D007814:Larva / Q000276:immunology; D051379:Mice; D012547:Schistosoma / Q000276:immunology; D012548:Schistosoma haematobium / Q000276:immunology; D012550:Schistosoma mansoni / Q000254:growth & development / Q000276:immunology*; D013045:Species Specificity
D000910:Antibodies, Heterophile; D000947:Antigens, Helminth; D000954:Antigens, Surface; D000939:Epitopes; D007074:Immunoglobulin G; D007075:Immunoglobulin M