Effects of aprotinin on hemostatic mechanisms during cardiopulmonary bypass.
Cardiopulmonary bypass (CPB) is associated with activation of humoral systems, which results in the release of proteases. These proteases may affect platelets and stimulate granulocytes. In the present study, the protease inhibitor aprotinin was given in high doses to 11 patients to achieve plasma concentrations of more than 150 kallikrein inactivator units per milliliter during CPB. At such concentrations, kallikrein and plasmin are effectively inhibited. This treatment resulted in platelet preservation during CPB. Platelet numbers were virtually unaffected, and thromboxane release was prevented in the aprotinin-treated group in contrast to the control group. Postoperatively, hemostasis was significantly better preserved after aprotinin treatment (blood loss of 357 ml in the treated group versus 674 ml in the untreated group; p less than 0.01). Since tissue-plasminogen activator activity was similar in both groups, the improved hemostasis most likely should be attributed to platelet preservation. Furthermore, aprotinin lessened neutrophilic elastase release, which might contribute to decreased pulmonary dysfunction in patients at risk.
D007611:Aprotinin / Q000008:administration & dosage / Q000097:blood / Q000627:therapeutic use*; D002315:Cardiopulmonary Bypass; D002986:Clinical Trials as Topic; D003167:Complement Activation / Q000187:drug effects; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis; D006487:Hemostasis / Q000187:drug effects*; D006801:Humans; D007262:Infusions, Intravenous; D007430:Intraoperative Care; D007958:Leukocyte Count / Q000187:drug effects; D008875:Middle Aged; D010196:Pancreatic Elastase / Q000097:blood; D010976:Platelet Count / Q000187:drug effects; D011897:Random Allocation; D013929:Thromboxane B2 / Q000097:blood; D010959:Tissue Plasminogen Activator / Q000097:blood
D005338:Fibrin Fibrinogen Degradation Products; D013929:Thromboxane B2; D007611:Aprotinin; D010196:Pancreatic Elastase; D010959:Tissue Plasminogen Activator