The combined use of limited proteolysis and differential peptide staining for protein characterization.
A method to characterize a protein by peptide mapping is described. It involves a combination of limited proteolysis and differential staining with the dye 'stains all' which allows the identification of the proteolytic fragments by their size and colour. When this procedure was used for tubulin, specific acidic fragments were identified and localized in the molecule.
D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D010446:Peptide Fragments / Q000032:analysis*; D010447:Peptide Hydrolases; D011506:Proteins / Q000032:analysis*; D013194:Staining and Labeling; D014404:Tubulin / Q000032:analysis
D010446:Peptide Fragments; D011506:Proteins; D014404:Tubulin; D010447:Peptide Hydrolases