Site-directed cleavage of RNA.
Using complementary chimeric oligonucleotides containing deoxyribonucleotides and 2'-O-methylribonucleotides (1), enzymatically synthesized RNA (90 mer) were cleaved at a single site with Escherichia coli RNaseH, either at a hairpin loop or at a stem region. Especially, site-specific cleavage occurred in even the target region being enclosed within a stable, base-paired stem. The method is proved to be generally applicable to RNA containing secondary structures.
D001483:Base Sequence; D004722:Endoribonucleases; D006860:Hydrogen Bonding; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides; D011777:Q beta Replicase / Q000378:metabolism; D012313:RNA / Q000032:analysis*; D012330:RNA, Double-Stranded / Q000378:metabolism; D016914:Ribonuclease H
D009843:Oligoribonucleotides; D012330:RNA, Double-Stranded; D012313:RNA; D011777:Q beta Replicase; D004722:Endoribonucleases; D016914:Ribonuclease H