Augmented mitogenic responsiveness to epidermal growth factor in murine fibroblasts that overexpress pp60c-src.
C3H10T1/2 murine fibroblasts overexpressing chicken pp60c-src showed a two- to fivefold enhanced incorporation of [3H]thymidine into DNA in response to epidermal growth factor (EGF) relative to that of the parent line. No difference in growth characteristics, number and affinity of EGF receptors, or hormone potency was attributable to c-src overexpression. These results suggest that pp60c-src may interact with the mitogenic signal transduction pathway of EGF in some event distal to hormone binding.
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects*; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D066246:ErbB Receptors / Q000378:metabolism; D051379:Mice; D008938:Mitosis / Q000187:drug effects; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D016392:Proto-Oncogene Proteins pp60(c-src)
D011518:Proto-Oncogene Proteins; D004815:Epidermal Growth Factor; D004247:DNA; D066246:ErbB Receptors; D016392:Proto-Oncogene Proteins pp60(c-src)