Non-selective cationic channel in primary cultured cells of brown adipose tissue.
Single channel current events were recorded from membranes of cultured brown adipose tissue cells with the patch-clamp technique. In excised inside-out patches the predominant type of events showed a slope conductance of about 30 pS (25 degrees C). The current was carried by both, Na+ ions and K+ ions. Discrimination between them was poor. The frequency of events increased with increasing temperature. Their amplitude was temperature dependent as well (Q10 approximately equal to 1.4). A single exponential was not sufficient for fitting the histograms of "on-time" or "off-time". We conclude that these events belong to a type of non-selective cation channel described previously for other tissues.
D002001:Adipose Tissue, Brown / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D004594:Electrophysiology; D007473:Ion Channels / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D013696:Temperature
D007473:Ion Channels; D012964:Sodium; D011188:Potassium