Effect of anti-Ia monoclonal antibodies on in vitro immune responses of canine cells.
Four murine monoclonal antibodies (MAB) B1F6, ISCR3, HB10a, and 7.2, specific for framework determinants of murine and human Ia, were tested for their effect on mixed lymphocyte culture, cell-mediated lympholysis, and mitogen assays of canine cells. All 4 MAB were reactive with monocytes and surface immunoglobulin-positive, as well as Thy-1 antigen-positive mononuclear cells, as determined by immunocytofluorescence. The mixed lymphocyte culture response was inhibited when B1F6 or HB10a was added to cultures at the beginning of the assay (day 0). However, cultures underwent a normal response in the presence of ISCR3 or 7.2. None of the MAB was able to inhibit cell-mediated lympholysis or mitogen reactivity to concanavalin A, pokeweed mitogen, or phytohemagglutinin. When effector cell populations of the 3 assays were cytolytically treated with each of the 4 MAB and complement, immune reactivity was consistently lost, indicating that effector cells or accessory cells supporting the cell populations had been eliminated. These data indicate that Ia-like antigens are expressed on canine mononuclear cells and that antigens recognized by different MAB may have different functional relevance, as defined by the mixed lymphocyte culture assay.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002469:Cell Separation; D002478:Cells, Cultured; D004285:Dogs; D000939:Epitopes; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D007111:Immunity, Cellular; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology; D009000:Monocytes / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D000949:Histocompatibility Antigens Class II