[Vital fluorochrome staining of isolated pancreatic acinar cells for the characterization of cell-structural changes].
Rhodamine 6 G as a cationic fluorophore is demonstrated to be selectively accumulated by mitochondria of living pancreatic acinar cells (cell isolation see Spormann et al. [1986]. The accumulation of rhodamine was studied under using of electron transport inhibitors, ionophores and some hydrogen donors. The application of DNP as wellknown protonophore resulted in a rapid dissipation of any fluorescent signals, whereas application of sodium succinate, hyperosmolaric exhibited a remarkable increase of fluorescence intensity. Using this technique it is possible to estimate the energy state of living cells under various conditions of energy supply and demand.
D000818:Animals; D004579:Electron Transport; D006168:Guinea Pigs; D010179:Pancreas / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D012235:Rhodamines; D013194:Staining and Labeling
D012235:Rhodamines; C026188:rhodamine 6G
Vitalfluorochromierung von isolierten Azinuszellen des Pankreas zur Charakterisierung des Zellstoffwechsels.