title: Selective osteoblast mitogens can be extracted from prostatic tissue.
abstract: Extracts of human prostatic carcinoma and of human benign prostatic hyperplasia were found to exhibit mitogenic activity in freshly isolated rat osteoblasts, rat fibroblasts, and osteoblast-derived osteosarcoma cells. This activity was markedly reduced by tryptic digestion of extracts, indicating its protein nature. Results of bioassay studies suggested that the prostatic material was different from a number of known growth factors. Analysis by reversed-phase high performance liquid chromatography revealed that extracts contained mitogenic activity for osteoblasts that was distinct from fibroblast-stimulating activity. Such osteoblast growth factors could form the basis of the skeletal osteogenic response to metastatic prostatic adenocarcinoma.
mesh_terms: D000230:Adenocarcinoma / Q000032:analysis; D000818:Animals; D002455:Cell Division; D002460:Cell Line; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D008297:Male; D008934:Mitogens / Q000302:isolation & purification*; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology; D011467:Prostate / Q000032:analysis*; D011470:Prostatic Hyperplasia / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013936:Thymidine / Q000378:metabolism; D014020:Tissue Extracts
chemical_list: D006133:Growth Substances; D008934:Mitogens; D014020:Tissue Extracts; D013936:Thymidine