Determination of soluble antigens of Clostridium botulinum A by chemiluminescent--immunosorbent assay (CLISA).
The chemiluminescent--immunosorbent assay (CLISA) was adopted for Cl. botulinum A soluble toxic antigens determination. Luminol (ABEI) labelled botulinum antitoxin globulins showed a strongly positive specific immunochemiluminescent reactions with the native botulinum toxin preparations coupled (adsorbed) on polystyrene balls. The sensitivity of the reaction reached 20 DLM/ml (5,000 light impulses per 40 sec) in comparison with 2 X 10(6) DLM (57,000 impulses), control preparations (1,500 impulses) and the background (150-300 impulses). The results give a perspective further investigations with the CLISA for rapid indication of Cl. botulinum toxic antigens.
D000942:Antigens, Bacterial / Q000032:analysis*; D001905:Botulinum Toxins / Q000032:analysis; D003014:Clostridium botulinum / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D008163:Luminescent Measurements / Q000379:methods; D008165:Luminol; D011137:Polystyrenes / Q000494:pharmacology; D011485:Protein Binding; D012995:Solubility
D000942:Antigens, Bacterial; D000939:Epitopes; D011137:Polystyrenes; D008165:Luminol; D001905:Botulinum Toxins