Immunocytochemical detection of parathyroid hormone-like substance in the goldfish brain and pituitary gland.
Parathyroid hormone (PTH)-like substance was immunocytochemically detected in the goldfish brain and pituitary gland using the peroxidase-antiperoxidase method with an antiserum raised against bovine PTH1(-84). Immunoreactive cell bodies were found in the nucleus preopticus in the preoptic area, and immunoreactive fibers could be traced from these cell bodies to the pituitary, in which immunoreactivity was detected in the neurohypophysis. These findings suggest that PTH-like substance is biosynthesized in the neuroendocrine cells in the brain and released in the neurohypophysis in the pituitary gland. Our results are discussed in relation to the hypercalcemic regulation of the pituitary gland in teleosts.
D000818:Animals; D000918:Antibody Specificity; D001921:Brain / Q000378:metabolism*; D003530:Cyprinidae / Q000502:physiology*; D000939:Epitopes; D006054:Goldfish / Q000502:physiology*; D007150:Immunohistochemistry; D010281:Parathyroid Hormone / Q000378:metabolism*; D010904:Pituitary Gland, Posterior / Q000378:metabolism*
D000939:Epitopes; D010281:Parathyroid Hormone