Recognition of specific targets by cultured dorsal root ganglion neurons.
We have assessed the effects of different target cell populations on axonally transported proteins by the use of compartmental cell culture systems that separate the soma from the growing axons of rat sensory neurons. The labeling of 3 rapidly transported proteins diminishes when the growing axon contacts spinal cord cells (which are normal in vivo targets), and remains unaffected by contact with fibroblasts or heart cells. Medium conditioned by spinal cord cells does not exert this effect. Thus, specific classes of cells may be distinguished as target tissue by sensory neurons in vitro. Such recognition is accompanied by specific molecular changes in axonally transported proteins.
D000818:Animals; D001370:Axonal Transport; D001369:Axons / Q000502:physiology; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000502:physiology; D005727:Ganglia, Spinal / Q000196:embryology / Q000502:physiology*; D006321:Heart / Q000502:physiology; D008715:Methionine; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009475:Neurons, Afferent / Q000502:physiology*; D051381:Rats; D013116:Spinal Cord / Q000502:physiology
D009419:Nerve Tissue Proteins; D008715:Methionine