Characterization of monoclonal antibodies directed against the TSH receptor, as revealed with the cytochemical bioassay.
The monoclonal antibody 11E8 (Kohn et al. 1984) is a novel proof of the actions of thyroid stimulator, since it is not only a potent stimulator of thyroid cells but also an inhibitor for TSH-binding. This might reflect an interaction of 11E8 with a discrete domaine of the TSH receptor, which is an essential component for TSH but not for TSab actions. These unique properties of 11E8 can be applied to the characterization of other thyroid stimulators which may interact with different domaines of the receptor. Moreover, 'TSab-like' stimulators, such as the sheep anti-TSH antiserum, could potentially be identified by 11E8.
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000939:Epitopes / Q000032:analysis; D006801:Humans; D011989:Receptors, Thyrotropin / Q000032:analysis* / Q000276:immunology; D013961:Thyroid Gland / Q000276:immunology
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000939:Epitopes; D011989:Receptors, Thyrotropin