Regulation of synthesis of N-linked glycoproteins and their lipid intermediates in human T lymphoblastoid cells.
The synthesis of N-linked glycoproteins and their lipid intermediates was investigated in cell-free preparations of human T lymphoblastoid cells during two phases of cell growth. The incorporation of 14C-labeled Man into glycoproteins and dolichol-linked oligosaccharides was greater during the logarithmic growth phase than the stationary phase. The incorporation of 14C-labeled GlcNAc into dolichol derivatives was increased in the logarithmic phase. However, the synthesis of Dol-P-Man was not significantly different. These data suggest that the differences are due, at least partially, to the increased synthesis of Dol-P-P-GlcNAc.
D002250:Carbon Radioisotopes; D002460:Cell Line; D004287:Dolichol Monophosphate Mannose / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis*; D006155:Guanosine Diphosphate Mannose / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D011104:Polyisoprenyl Phosphate Oligosaccharides / Q000096:biosynthesis*; D011105:Polyisoprenyl Phosphate Sugars / Q000096:biosynthesis* / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism*
D002250:Carbon Radioisotopes; D006023:Glycoproteins; D011104:Polyisoprenyl Phosphate Oligosaccharides; D011105:Polyisoprenyl Phosphate Sugars; D006155:Guanosine Diphosphate Mannose; D004287:Dolichol Monophosphate Mannose