Characterization of RNA from Leishmania tropica and Leishmania d.donovani promastigotes.
RNA has been prepared from promastigotes of Leishmania tropica and Leishmania d.donovani using three different methods. Extraction by hot phenol/isothiocyanate gave the best quantitative and qualitative results. The analysis of total RNA on methyl mercuric agarose gels shows that the large rRNA species is nicked: it is composed of a 630 and a 560 kDa molecule. The small rRNA species has a molecular weight of 800,000. Poly(A+) RNA can be translated in a rabbit reticulocyte lysate system. The newly synthesized products comprise high molecular weight proteins and show different patterns using RNA from L. tropica or from L. d. donovani promastigotes.
D000818:Animals; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D007893:Leishmania donovani / Q000235:genetics*; D007895:Leishmania tropica / Q000235:genetics*; D011061:Poly A / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D014176:Protein Biosynthesis; D012313:RNA / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D012335:RNA, Ribosomal / Q000032:analysis
D012333:RNA, Messenger; D012335:RNA, Ribosomal; D011061:Poly A; D012313:RNA