Studies on the Vibrio cholerae mucinase complex. III. Neutralisation of the neuraminidase activity by specific anti-neuraminidase IgG.
A partially-purified neuraminidase from the mucinase complex of Vibrio cholerae was used to prepare a specific anti-neuraminidase antiserum in rabbits. When the neutralising potency of this serum against V. cholerae neuraminidase was assessed in conventional tests, the enzymic activity, as measured by thiobarbituric acid, methoxyphenol-neuraminate and goblet-cell assays, apparently increased. These results are attributable to the presence of a sialylated glycoprotein substrate and small amounts of sialidase in the crude antiserum. However, a twice-purified DEAE-IgG fraction of the antiserum neutralised the enzymic activity of the V. cholerae neuraminidase.
D000423:Alcian Blue; D000818:Animals; D007082:Ileum / Q000032:analysis; D007074:Immunoglobulin G / Q000276:immunology*; D007413:Intestinal Mucosa / Q000032:analysis; D009439:Neuraminidase / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000378:metabolism; D009500:Neutralization Tests; D051381:Rats; D013194:Staining and Labeling; D014734:Vibrio cholerae / Q000201:enzymology*
D007074:Immunoglobulin G; D009439:Neuraminidase; D000423:Alcian Blue