A simple method for histochemical demonstration of viral inclusion bodies in cell monolayers in microtitration plates.
The present paper describes a Bouin's-formalin fixation and Giemsa staining procedure for demonstrating viral cytoplasmic inclusion bodies in cellular monolayers in microtitration plates. Monolayers are fixed in Bouin's fixative for 15 min followed by 10% neutral buffered formalin fixation overnight. After fixation, the monolayers are stained with 4% (v/v) Giemsa stain. The method is superior to the separate use of formalin, methanol or Bouin's fixation-staining methods and compares favorably to immunocytochemical techniques for sensitivity.
D002460:Cell Line; D002478:Cells, Cultured / Q000382:microbiology; D005404:Fixatives; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry / Q000379:methods; D007181:Inclusion Bodies, Viral / Q000648:ultrastructure*; D018108:Respirovirus / Q000032:analysis; D013194:Staining and Labeling
D005404:Fixatives