The T 11 (CD 2) cDNA encodes a transmembrane protein which expresses T 11(1), T 11(2) and T 11(3) epitopes but which does not independently mediate calcium influx: analysis by gene transfer in a baculovirus system.
To determine whether a full-length human T 11 (CD 2) cDNA encodes a protein which expresses all three T 11 epitopes (T 11(1), T 11(2), T 11(3] and independently triggers activation in a cell other than a T lymphocyte, a baculovirus expression system was employed. Here we show that a recombinant T 11 cDNA-containing baculovirus can induce high-level expression of T 11(1), T 11(2) and T 11(3) epitopes on the surface of gut epithelial SF9 cells. However, in this environment the T 11 protein cannot be triggered to transduce a signal resulting in an elevation of the cytosolic free Ca2+ as is known to occur in T lymphocytes. These results support the notion that T 11 functions in a coordinate fashion with other intracellular lymphoid components.
D000818:Animals; D018801:CD2 Antigens; D002118:Calcium / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D000939:Epitopes / Q000235:genetics*; D005822:Genetic Vectors; D006801:Humans; D007304:Insect Viruses / Q000235:genetics; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology; D007473:Ion Channels / Q000378:metabolism; D007915:Lepidoptera; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology; D011971:Receptors, Immunologic / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000502:physiology
D018801:CD2 Antigens; D002352:Carrier Proteins; D000939:Epitopes; D007473:Ion Channels; D008565:Membrane Proteins; D011971:Receptors, Immunologic; D011993:Recombinant Fusion Proteins; D004247:DNA; D002118:Calcium