Description of an automated assay for measurement of alpha-amylase in vitro from rat parotid gland slices.
1. A modified continuous assay of alpha-amylase is detailed and its usefulness in measuring amylase secretion from rat parotid salivary gland described. 2. The alpha-amylase secretion from the rat parotid gland can be evoked by acetylcholine, substance P and isoprenaline. 3. The secretion of amylase was greatest following isoprenaline stimulation and was of a slower time course than with the other two agonists.
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001322:Autoanalysis; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D010306:Parotid Gland / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D013373:Substance P / Q000494:pharmacology; D000516:alpha-Amylases / Q000378:metabolism*
D013373:Substance P; D000516:alpha-Amylases; D007545:Isoproterenol; D000109:Acetylcholine