Comparison of histamine release and prostaglandin E2 production of human basophils in atopic and normal individuals.
The influence of arachidonic acid (AA) metabolism upon histamine release (HR) from human basophils after stimulation with anti-IgE was studied in 23 atopic and 11 normal individuals. HR occurred significantly faster in atopics than in normals; the total amount of HR after a 40 min incubation period was not significantly different between the two groups. Indomethacin and acetylsalicylic acid (ASA) increased the quantity of HR significantly both in atopics and normals without influencing the time course. Addition of exogenous PGE2 decreased HR; here atopics were more affected than normals 5 and 10 min after challenge with anti-IgE. Production of PGE2 after stimulation with anti-IgE was very low in both groups (in the range of 30-50 pg/10(6) cells) and often below detection limit (10-20 pg/ml). Addition of glutathione (GSH), a coenzyme of PGE2-isomerase, increased PGE2 production 2 to 5-fold during stimulation with anti-IgE. These data support the idea that arachidonic acid metabolites play an important role in modulating the "releasability" of human basophils. It is suggested that the basophils of atopic individuals may release their histamine faster than normals - perhaps on the basis of a more slowly acting endogenous feedback mechanism by PGE2. Both phenomena support the idea of an altered "releasability" of basophils from atopics compared to normals.
D000293:Adolescent; D000328:Adult; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001095:Arachidonic Acids / Q000378:metabolism*; D001491:Basophils / Q000276:immunology / Q000378:metabolism*; D005260:Female; D005978:Glutathione / Q000494:pharmacology; D006636:Histamine Release; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000378:metabolism*; D007073:Immunoglobulin E / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011458:Prostaglandins E / Q000096:biosynthesis*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D001095:Arachidonic Acids; D011458:Prostaglandins E; D007073:Immunoglobulin E; D005978:Glutathione