Effects of dextran sulfate on stabilization of milk lipoprotein lipase and VLDL triglyceride hydrolysis in vitro.
The effects of dextran sulfate (DS), which has various molecular numbers, on hydrolysis of very low density lipoprotein triglyceride (VLDL-TG) by bovine milk lipoprotein lipase (LPL) and the stability of LPL were studied. VLDL-TG hydrolysis was increased by the addition of DS; DS caused linear increase in the Vmax for VLDL-TG with increase in its sulfate content, but did not change the Km value for VLDL-TG. DS also stabilized LPL, but this effect was not dependent on its sulfate content. These results suggest that the mechanism of action of DS in LPL stabilization may be different from that in enhancement of VLDL hydrolysis.
D000818:Animals; D002417:Cattle; D016264:Dextran Sulfate; D003911:Dextrans / Q000494:pharmacology*; D066298:In Vitro Techniques; D007700:Kinetics; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008079:Lipoproteins, VLDL / Q000378:metabolism*; D008892:Milk / Q000201:enzymology*; D008970:Molecular Weight; D013329:Structure-Activity Relationship; D014280:Triglycerides / Q000378:metabolism*
D003911:Dextrans; D008079:Lipoproteins, VLDL; D014280:Triglycerides; D016264:Dextran Sulfate; D008071:Lipoprotein Lipase