Functional analysis of the HLA-A2/Aw68 recombinant molecule HLA-Aw69: Epstein-Barr virus specific cytotoxic T cells restricted through Aw69 can utilise restricting determinants on Aw68 but not on A2.
Epstein-Barr virus-specific cytotoxic T cell lines, expanded in interleukin 2-conditioned medium, have been generated in vitro from virus-immune individuals bearing either the common HLA-A2 antigen (A2.1), a variant A2 antigen (A2.2) or a novel antigen Aw69 which is a recombinant molecule having identity with HLA-Aw68 in the alpha 1 domain and with HLA-A2 in the alpha 2 and alpha 3 domains. Virus-specific cytotoxic T cells restricted through A2.1 or A2.2 only recognised targets bearing the identical sub-type of A2 molecules and showed no cross-recognition of other A2 sub-types, of Aw68 or of Aw69. In contrast, virus-specific cytotoxic T cells restricted through the Aw69 antigen showed a significant cross-recognition of restricting determinants on Aw68 but not on A2 molecules. The results suggest that the alpha 1 extracellular domain of the HLA-Aw69 molecule plays a predominant role in the formation of T cell restricting determinants.
D000911:Antibodies, Monoclonal; D002472:Cell Transformation, Viral; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology*; D006680:HLA Antigens / Q000235:genetics / Q000276:immunology*; D015234:HLA-A Antigens; D015789:HLA-A2 Antigen; D004854:Herpesvirus 4, Human; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D011994:Recombinant Proteins / Q000032:analysis*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006680:HLA Antigens; D015234:HLA-A Antigens; D015789:HLA-A2 Antigen; C037164:HLA-A28 antigen; D007376:Interleukin-2; D011994:Recombinant Proteins