Identification of a phosphoprotein in the nuclear matrix by monoclonal antibodies.
Previous work in this laboratory has established that a rat liver nuclear phosphoprotein (B2:Mr 68,000, pI 6.5-8.2) is associated with actively transcribed nucleosomes, as demonstrated by its preferential release after mild treatment with micrococcal nuclease. In the present report we provide further immunological evidence ('Western Blot' analysis, solid-phase radioimmunoassay and indirect immunofluorescence) that in addition establishes the presence of this phosphoprotein in the nuclear-matrix protein fraction. This paradoxical localization suggests that this phosphoprotein may function in two separate and distinct roles within the realm of nuclear organization.
D000818:Animals; D000911:Antibodies, Monoclonal; D002868:Chromosomal Proteins, Non-Histone / Q000276:immunology / Q000302:isolation & purification*; D000939:Epitopes / Q000032:analysis; D005455:Fluorescent Antibody Technique; D007122:Immunoelectrophoresis; D008099:Liver / Q000032:analysis*; D008297:Male; D010750:Phosphoproteins / Q000276:immunology / Q000302:isolation & purification*; D011485:Protein Binding; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains
D000911:Antibodies, Monoclonal; D002868:Chromosomal Proteins, Non-Histone; D000939:Epitopes; D010750:Phosphoproteins; C041883:nuclear phosphoprotein B2, rat