51Cr-uptake assay. A sensitive and reliable method to quantitate cell viability and cell death.
This report describes a sensitive and reproducible cytotoxicity assay which is ideal for screening toxic compounds or soluble lytic factors from leukocytes. The assay relies on the ability of sodium [51Cr]chromate to be taken up by viable but not dead cells. This assay is the reverse of the conventional 51Cr-release assay. The target cells are 51Cr-labeled after incubation with cytotoxic factors. 51Cr-uptake does not depend on DNA, RNA or protein synthesis and the only parameters which influence its uptake are temperature, viability, number and cell volume.
D001386:Azides / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival; D002860:Chromium Radioisotopes / Q000378:metabolism*; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D003609:Dactinomycin / Q000494:pharmacology; D006801:Humans; D007942:Leukemia, Experimental / Q000378:metabolism; D007951:Leukemia, Myeloid / Q000378:metabolism; D008722:Methods; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D019810:Sodium Azide
D001386:Azides; D002860:Chromium Radioisotopes; D008937:Mitomycins; D003609:Dactinomycin; D016685:Mitomycin; D019810:Sodium Azide