Characterization and genetic mapping of a mutation affecting apurinic endonuclease activity in Staphylococcus aureus.
Protoplast fusion between the Rec- mutant RN981 (L. Wyman, R. V. Goering, and R. P. Novick, Genetics 76:681-702, 1974) of Staphylococcus aureus NCTC 8325 and a Rec+ NCTC 8325 derivative yielded Rec+ recombinants that exhibited the increased sensitivity to N-methyl-N'-nitro-N-nitrosoguanidine characteristic of RN981. Transformation analyses identified a specific mutation, designated ngr-374, that was responsible not only for N-methyl-N'-nitro-N-nitrosoguanidine sensitivity, but also sensitivity to methyl methanesulfonate, ethyl methanesulfonate, nitrous acid, and UV irradiation. However, ngr-374-carrying recombinants showed no significant increase in their sensitivity to mitomycin C or 4-nitroquinoline 1-oxide and were unaffected in recombination proficiency. In vitro assays showed that ngr-374-carrying strains had lower apurinic/apyrimidinic endonuclease activities than the wild type. The chromosomal locus occupied by ngr-374 was shown to exist in the gene order omega(Chr::Tn551)40-ngr-374-thrB106.
D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004260:DNA Repair; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D043223:Deoxyribonuclease IV (Phage T4-Induced); D004706:Endodeoxyribonucleases / Q000235:genetics / Q000378:metabolism*; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D005798:Genes, Bacterial; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D016685:Mitomycin; D008937:Mitomycins / Q000494:pharmacology; D009154:Mutation; D009608:Nitrous Acid / Q000494:pharmacology; D011995:Recombination, Genetic; D013211:Staphylococcus aureus / Q000201:enzymology / Q000235:genetics*; D014466:Ultraviolet Rays
D008937:Mitomycins; D008769:Methylnitronitrosoguanidine; D016685:Mitomycin; D015112:4-Nitroquinoline-1-oxide; D005020:Ethyl Methanesulfonate; D008741:Methyl Methanesulfonate; D004706:Endodeoxyribonucleases; D043223:Deoxyribonuclease IV (Phage T4-Induced); D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D009608:Nitrous Acid