Cell-mediated immunoreactivity of tumor-bearing mice with myelin basic protein and related peptides.
Spleen cells (SC) from tumor-bearing mice and mice immunized with porcine myelin basic protein (MBP) reacted in vitro in E-rosette augmentation assays with MBP and certain of its constituent peptides. Peptides 1-115, 43-169, 64-83, 113-121, and 153-161 reacted significantly with both types of SC, while peptide 1-19 reacted only with SC from MBP-immunized mice. The phenomenon of specific inhibition of peptide reactivity by a moderate excess of a related protein was used to identify peptides as accessible epitopes of that protein. Peptide 113-121 was specifically inhibited by excess MBP when reacted with both types of SC, whereas peptide 64-83 was inhibited by excess MBP only when reacted with SC from MBP-immunized mice. These reactions suggest that the immunizing antigen in tumor-bearing mice is related to MBP but differs in epitopes associated with peptides 1-19 and 64-83.
D000595:Amino Acid Sequence; D000818:Animals; D004306:Dose-Response Relationship, Immunologic; D005354:Fibrosarcoma / Q000276:immunology; D006168:Guinea Pigs; D007111:Immunity, Cellular; D007114:Immunization; D051379:Mice; D008808:Mice, Inbred CBA; D004676:Myelin Basic Protein / Q000235:genetics / Q000276:immunology*; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology*; D051381:Rats; D011917:Rats, Inbred Lew; D012397:Rosette Formation; D013154:Spleen / Q000166:cytology
D004676:Myelin Basic Protein