Severe hemolytic disease of the newborn due to anti-Vw and detection of glycophorin A antigens on the Miltenberger I sialoglycoprotein by Western blotting.
Anti-Vw detecting an antigen on Miltenberger I (Mi I) variant glycophorin A (GPA) has rarely been reported as a cause of hemolytic disease of the newborn (HDN). We report an infant with severe HDN due to anti-Vw. Examination of the Vw+ erythrocytes of the father and paternal grandmother by sodium dodecylsulphate polyacrylamide gel electrophoresis showed an extra trypsin-sensitive, periodic-acid-Schiff staining band, consistent with Mi I variant GPA. Staining of Western blots by monoclonal antibodies showed that normal paternal GPA expressed blood group M, while Mi I variant GPA expressed blood group N. Mi I variant GPA expressed the trypsin-sensitive antigenic determinant detected by MoAb 10F7, indicating that the alterations known to occur in the trypsin-sensitive fragment of Mi I variant GPA do not affect expression of the antigen detected by 10F7.
D000743:Anemia, Hemolytic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D001788:Blood Grouping and Crossmatching; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis*; D005260:Female; D014644:Genetic Variation; D006021:Glycophorins / Q000235:genetics; D006801:Humans; D007231:Infant, Newborn; D008951:MNSs Blood-Group System / Q000276:immunology*; D012795:Sialoglycoproteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006021:Glycophorins; D008951:MNSs Blood-Group System; D012795:Sialoglycoproteins; D003101:Collodion