Polymorphism of lymphocyte function-associated antigen-1 demonstrated by a lupus patient's alloantiserum.
We have found a human serum, E27, obtained from a multiply transfused patient with systemic lupus erythematosus, which immunoprecipitates the lymphocyte function associated antigen-1 (LFA-1). The immunoprecipitated molecules were identified as the LFA-1 alpha and beta chains by their comigration on SDS-PAGE, two-dimensional SDS-PAGE, and by sequential clearance experiments. Serum E27 did not immunoprecipitate LFA-1 from autologous cells, though LFA-1 molecules were present. In contrast, serum E27 immunoprecipitated LFA-1 from most but not all normal donor lymphocytes. Thus, serum E27 defines two serological phenotypes of LFA-1. 95% of normal individuals tested exhibited the LFA-1 phenotype precipitated by serum E27. Serum E27 appears to be directed at determinants of the LFA-1 alpha-chain and not the beta-chain since it immunoprecipitated LFA-1 molecules but not the Mac-1 molecules. Additional evidence for the alpha chain specificity was provided by immunoprecipitation of mouse-human heterohybridoma cells. LFA-1 was immunoprecipitated by serum E27 from mouse-human heterohybridoma cells expressing the human alpha-chain, not from a hybrid cell line expressing the human beta-chain. Together these findings demonstrate an antigenic polymorphism of the human LFA-1 alpha-chain molecule.
D000954:Antigens, Surface / Q000145:classification / Q000235:genetics / Q000276:immunology*; D001323:Autoantibodies / Q000276:immunology; D001803:Blood Transfusion; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D006801:Humans; D007518:Isoantibodies / Q000276:immunology*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D016169:Lymphocyte Function-Associated Antigen-1; D011110:Polymorphism, Genetic
D000954:Antigens, Surface; D001323:Autoantibodies; D000939:Epitopes; D007518:Isoantibodies; D016169:Lymphocyte Function-Associated Antigen-1