Sex determination from buccal mucosa and hair root by the combined treatment of quinacrine staining and the fluorescent Feulgen reaction using a single specimen.
In order to determine sex using a single specimen, buccal mucosa and hair roots obtained from male and female individuals were used. The specimens were first stained with quinacrine for the detection of the Y-chromatin, and subsequently were stained by the fluorescent Feulgen reaction using acriflavine for the detection of the X-chromatin. In the male specimens, the frequency of fluorescent spots of quinacrine-positive bodies was high, whereas that of acriflavine-positive spots was low. On the other hand, in the female specimens, the frequency of quinacrine-positive spots was very low, while that of the acriflavine-positive spots was high. These specimens were air-dried and were allowed to stand at room temperature for periodical observations. The result was that sex difference was distinguishable for 4 months by the combined treatment method.
D004396:Coloring Agents; D005260:Female; D005456:Fluorescent Dyes; D006197:Hair / Q000648:ultrastructure*; D006801:Humans; D008297:Male; D009061:Mouth Mucosa / Q000648:ultrastructure*; D011796:Quinacrine; D012394:Rosaniline Dyes; D012732:Sex Determination Analysis; D013194:Staining and Labeling; D014960:X Chromosome / Q000648:ultrastructure; D014998:Y Chromosome / Q000648:ultrastructure
D004396:Coloring Agents; C007722:Feulgen stain; D005456:Fluorescent Dyes; D012394:Rosaniline Dyes; D011796:Quinacrine