Electrophoretic identification of fusion proteins expressed in single recombinant lambda-bacteriophage plaques.
Sufficient fusion protein is present in single plaques produced by lytic, recombinant lambda bacteriophage to be detected by Coomassie blue staining following electrophoresis on sodium dodecyl sulfate-polyacrylamide gels. Agar plugs containing single plaques can be loaded directly onto the stacking gel thus avoiding the need for extensive sample preparation.
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D010963:Plasmodium falciparum / Q000235:genetics; D011994:Recombinant Proteins / Q000032:analysis; D012394:Rosaniline Dyes; D013194:Staining and Labeling; D014764:Viral Proteins / Q000032:analysis*
D004279:DNA, Viral; D011994:Recombinant Proteins; D012394:Rosaniline Dyes; D014764:Viral Proteins; C048139:Coomassie blue