title: Quantitative ultrastructural autoradiographic study of RNA transport in rat ventral prostate.
abstract: The nucleocytoplasmic RNA transport in rat ventral prostate was studied by electron microscope autoradiography. Isolated prostate acini from normal, castrated, and DHT-treated animals were labeled in vitro with [3H]uridine for 5 min and chased for 0, 15, 30 min and 4 hr. The results show that DHT induces a significant nucleolar enlargement but intranuclear migration of rRNA is not apparently affected by androgens; migration of RNA through euchromatin is delayed by castration and stimulated by DHT; migration through the nuclear envelope is androgen-dependent. In addition prostate acini were maintained for 24 hr in suspension culture in order to study the in vitro effects of DHT. The result show that DHT stimulates uridine uptake and/or incorporation but induces no nucleolar enlargement; DHT has no clear effects on RNA migration kinetics; cytoplasmic transport of RNA in cells cultured in medium with or without DHT is severely impaired but is restored after supplementation of medium with insulin and dexamethasone.
mesh_terms: D000818:Animals; D001345:Autoradiography; D001692:Biological Transport; D002451:Cell Compartmentation; D002467:Cell Nucleus / Q000378:metabolism; D046508:Culture Techniques; D003593:Cytoplasm / Q000378:metabolism; D013196:Dihydrotestosterone / Q000494:pharmacology; D005938:Glucocorticoids / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D009919:Orchiectomy; D011467:Prostate / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D051381:Rats
chemical_list: D005938:Glucocorticoids; D007328:Insulin; D013196:Dihydrotestosterone; D012313:RNA