A new experimental model to study oxygen toxicity.
An experimental model was developed in order to study the protective effect of antioxidant molecules. Human diploid WI-38 fibroblasts were cultivated under 2 atm of 95% O2. Antioxidants like alpha-tocopherol or superoxide dismutase (SOD) were added respectively in the culture medium or directly inside the cell through a microinjection technique. With both antioxidant molecules a protection was observed. In the control experiment, cells died within 6 or 8 days depending on the confluency and the malonaldehyde content increased sharply. This model represents a new tool in order to test other antioxidant systems towards an oxidative stress.
D000975:Antioxidants / Q000494:pharmacology; D002460:Cell Line; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D008845:Microinjections; D010100:Oxygen / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology
D000975:Antioxidants; D014810:Vitamin E; D013482:Superoxide Dismutase; D010100:Oxygen