Identification of the sequence required for expression of the 2H4 epitope on the human leukocyte common antigens.
The anti-2H4 antibody, which subdivides the T4+ population of human T lymphocytes into T4+, 2H4+ suppressor-inducer cells and T4+, 2H4- helper cells, recognizes an epitope on a subset of the human leukocyte common antigens (LCAs). LCAs are a family of cell surface glycoproteins generated from a single gene by the differential usage of three exons near the NH2-terminus. Using cDNA clones corresponding to four of the different forms of LCA molecules, extracellular domains of the LCA molecules were synthesized in vitro. Immunoprecipitation of these molecules with the anti-2H4 antibody demonstrated that exon A is required for the expression of the 2H4 epitope.
D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D000939:Epitopes / Q000235:genetics* / Q000276:immunology; D005091:Exons; D006649:Histocompatibility Antigens / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007163:Immunosorbent Techniques; D017493:Leukocyte Common Antigens; D051379:Mice; D010957:Plasmids; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006649:Histocompatibility Antigens; D012333:RNA, Messenger; D004247:DNA; D017493:Leukocyte Common Antigens