Role of endothelium in haemoglobin-induced contraction of dog basilar artery.
Haemoglobin (10 nM-30 microM) caused contraction of dog isolated basilar artery. In preparations which were perfused with Triton X-100 (0.1%) for one min to remove the endothelium, substance P relaxation was abolished but haemoglobin-induced contractions were unchanged. Endothelium removal or damage was confirmed histologically. These results suggest that the integrity of the endothelium is not essential for haemoglobin to produce contraction in dog basilar artery.
D000818:Animals; D001488:Basilar Artery / Q000033:anatomy & histology / Q000187:drug effects; D004285:Dogs; D004727:Endothelium / Q000502:physiology*; D005260:Female; D006454:Hemoglobins / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000033:anatomy & histology / Q000187:drug effects*; D009599:Nitroprusside / Q000494:pharmacology; D017830:Octoxynol; D010477:Perfusion; D011092:Polyethylene Glycols / Q000494:pharmacology; D013373:Substance P / Q000494:pharmacology
D006454:Hemoglobins; D009599:Nitroprusside; D013373:Substance P; D011092:Polyethylene Glycols; D017830:Octoxynol