Isolation of novel human retrovirus-related sequences by hybridization to synthetic oligonucleotides complementary to the tRNA(Pro) primer-binding site.
Synthetic oligonucleotides complementary to putative retroviral primer-binding sites were used as hybridization probes to detect novel retroviruslike sequences. An 8.1-kilobase element with structural features of a retroviral provirus was isolated from a human genomic library by this approach. Nucleotide sequence analysis of its 600-base-pair long terminal repeats revealed characteristic motifs known as regulatory signals for RNA polymerase II transcription: CCAAT, TATA, and ATTAAA. In addition, a putative pol gene displays apparent homologies to conserved regions of retroviral reverse transcriptase. The 5' long terminal repeat is flanked at its 3' end by a putative primer-binding site for reverse transcription with homology to tRNA(Pro). This element is therefore termed HuRRS-P (human retrovirus-related sequence-proline). There are 20 to 40 copies of HuRRS-P homologous sequences in DNAs of human and simian origin.
D000818:Animals; D001665:Binding Sites; D004247:DNA / Q000235:genetics*; D004251:DNA Transposable Elements; D004274:DNA, Recombinant / Q000302:isolation & purification; D006801:Humans; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000235:genetics*; D010802:Phylogeny; D011323:Primates / Q000235:genetics; D012313:RNA / Q000235:genetics*; D012319:RNA Polymerase II / Q000378:metabolism; D012343:RNA, Transfer / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D012194:RNA-Directed DNA Polymerase / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D012190:Retroviridae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics
D004251:DNA Transposable Elements; D004274:DNA, Recombinant; D009838:Oligodeoxyribonucleotides; C034477:RNA primers; D012367:RNA, Viral; D014764:Viral Proteins; D012313:RNA; D004247:DNA; D012343:RNA, Transfer; D012319:RNA Polymerase II; D012194:RNA-Directed DNA Polymerase