Generation of macrophage variants with 5-azacytidine: selection for mannose receptor expression.
Mannose receptors are expressed only in primary macrophages. Established macrophage-derived cell lines, although apparently possessing the potential to synthesize mannose receptors, do not express them on their plasma membranes. Using the drug 5-Azacytidine, mannose receptor expression was induced in the macrophage-derived mouse cell line J774. Receptor positive cells were sorted through a fluorescent activated cell sorter (FACS) prior to cloning. Clones were isolated which continuously express mannose receptors in culture. These macrophages were able to endocytose beta-glucuronidase and phagocytose yeast particles via mannose receptors. Secretion of the lysosomal enzyme beta-hexosaminidase was also reduced in proportion to the degree of mannose receptor expression.
D000818:Animals; D001374:Azacitidine / Q000494:pharmacology*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002999:Clone Cells; D004705:Endocytosis; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D005786:Gene Expression Regulation / Q000187:drug effects; D014644:Genetic Variation; D005966:Glucuronidase / Q000378:metabolism; D037181:Lectins, C-Type; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D051379:Mice; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic / Q000235:genetics*; D013861:Thiocyanates
D005452:Fluoresceins; D005456:Fluorescent Dyes; D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D013861:Thiocyanates; D005966:Glucuronidase; D016650:Fluorescein-5-isothiocyanate; D001374:Azacitidine