Inhibition of bleomycin-induced DNA strand breaks in V 79 Chinese hamster cells by the antioxidant propylgallate.
Bleomycin induced DNA single-strand breaks in Chinese hamster V 79 cells which were detected by the alkaline filter elution assay. In the presence of propylgallate, an antioxidant, the amount of DNA single-strand breaks was significantly reduced. The production of DNA strand breaks by methylnitronitrosoguanidine used as a positive control was not influenced by propylgallate. It is suggested that propylgallate inhibits the generation of DNA single-strand breaks by trapping reactive oxygen species produced by the bleomycin-iron(II) complex.
D000818:Animals; D001761:Bleomycin / Q000037:antagonists & inhibitors*; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004277:DNA, Single-Stranded / Q000187:drug effects*; D005707:Gallic Acid / Q000031:analogs & derivatives*; D008769:Methylnitronitrosoguanidine / Q000633:toxicity; D011435:Propyl Gallate / Q000494:pharmacology*
D004277:DNA, Single-Stranded; D001761:Bleomycin; D008769:Methylnitronitrosoguanidine; D005707:Gallic Acid; D011435:Propyl Gallate