The N-terminal half of the heavy chain of botulinum type A neurotoxin forms channels in planar phospholipid bilayers.
The heavy chain of botulinum type A neurotoxin forms channels in planar phospholipid bilayer membranes. Channel activity is confined to the N-terminal half of this chain; the C-terminal half is inactive. Channel activity is stimulated by low pH (4.5-5.5) on the cis side (the side to which protein is added), neutral pH on the opposite (trans) side, and cis positive voltages. These findings are strikingly similar to those previously reported for analogous fragments of diphtheria and tetanus toxins.
D001905:Botulinum Toxins; D006863:Hydrogen-Ion Concentration; D007473:Ion Channels / Q000502:physiology; D008051:Lipid Bilayers; D046911:Macromolecular Substances; D008954:Models, Biological; D008970:Molecular Weight; D009498:Neurotoxins; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines
D007473:Ion Channels; D008051:Lipid Bilayers; D046911:Macromolecular Substances; D009498:Neurotoxins; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines; D001905:Botulinum Toxins