Identification of proteins by crossed immunoelectrophoresis with a trap-gel.
Simple improvements of the crossed immunoelectrophoresis method are described. A trap-gel was prepared with a small quantity of monospecific antiserum and was submitted to preelectrophoresis. It blocked, during the first dimension, an individual protein as a rocket. The corresponding peak was reduced or disappeared in the second dimension. Identification of precipitation peaks in human or Macacus cynomolgus bronchoalveolar lavage fluids is illustrated and unequivocal recognition of some antigens is shown.
D000818:Animals; D000990:Antithrombin III / Q000302:isolation & purification; D001980:Bronchi / Q000276:immunology; D003429:Cross Reactions; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods*; D007123:Immunoelectrophoresis, Two-Dimensional / Q000379:methods*; D007070:Immunoglobulin A / Q000302:isolation & purification; D008252:Macaca fascicularis; D011506:Proteins / Q000302:isolation & purification*; D011650:Pulmonary Alveoli / Q000276:immunology; D007507:Therapeutic Irrigation; D014168:Transferrin / Q000302:isolation & purification; D014809:Vitamin D-Binding Protein / Q000302:isolation & purification; D000511:alpha-Macroglobulins / Q000302:isolation & purification
D007070:Immunoglobulin A; D011506:Proteins; D014168:Transferrin; D014809:Vitamin D-Binding Protein; D000511:alpha-Macroglobulins; D000990:Antithrombin III