Novel serine phosphorylation of pp60c-src in intact cells after tumor promoter treatment.
Treatment of normal cells with the tumor promoters 12-O-tetradecanoylphorbol-13-acetate and mezerein results in increased phosphorylation of pp60c-src. Two-dimensional tryptic phosphopeptide analysis of partial V8 protease fragments indicated that this phosphorylation takes place on a serine residue which lies within the amino-terminal 18 kilodaltons of pp60c-src and represents the major phosphorylation site following tumor promoter treatment. Untreated cells exhibited a low but detectable level of phosphorylation at this serine residue. The significance of these results with respect to the phosphoregulation of pp60c-src as well as tumor promotion is discussed.
D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D002460:Cell Line; D004224:Diterpenes; D008168:Lung; D008907:Mink; D010748:Phosphopeptides / Q000032:analysis; D010766:Phosphorylation; D011401:Promoter Regions, Genetic; D011494:Protein Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D016392:Proto-Oncogene Proteins pp60(c-src); D012694:Serine; D013729:Terpenes / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014357:Trypsin
D002273:Carcinogens; D004224:Diterpenes; D010748:Phosphopeptides; D011518:Proto-Oncogene Proteins; D013729:Terpenes; C011309:mezerein; D012694:Serine; D011494:Protein Kinases; D016392:Proto-Oncogene Proteins pp60(c-src); D014357:Trypsin; D013755:Tetradecanoylphorbol Acetate