Epithelial ion transport in rabbit corneas following myopic keratomileusis.
In isolated rabbit corneas that had undergone lamellar keratectomy or myopic keratomileusis, the stimulation of chloride transport by 10(-5) M epinephrine was completely inhibited at 1 week following surgery. At 28 days following surgery, both groups responded to 10(-7) M epinephrine. The response to 10(-5) M amphotericin B was normal both at 1 week and at 28 days following surgery. We conclude that, although the Na-K pump was not affected by the lamellar keratectomy and cryolathing, that either the epithelial beta receptors and/or the cAMP pathway were temporarily inhibited for at least 1 week following surgery. A lamellar keratectomy, therefore, can have an adverse effect on the epithelial transport system of the corneal epithelium even though the epithelium may appear normal clinically.
D000666:Amphotericin B / Q000494:pharmacology; D000818:Animals; D001693:Biological Transport, Active; D002712:Chlorides / Q000378:metabolism; D003315:Cornea / Q000378:metabolism* / Q000601:surgery; D000242:Cyclic AMP / Q000378:metabolism; D004837:Epinephrine / Q000494:pharmacology; D004848:Epithelium / Q000378:metabolism; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D009216:Myopia / Q000601:surgery*; D011817:Rabbits; D011943:Receptors, Adrenergic, beta / Q000378:metabolism
D002712:Chlorides; D007473:Ion Channels; D011943:Receptors, Adrenergic, beta; D000666:Amphotericin B; D000242:Cyclic AMP; D004837:Epinephrine