HLA-DR antigens in epithelioid cell granulomas of sarcoidosis, using semithin frozen sections. A concept about granuloma formation.
Mediastinal lymph nodes of 11 patients with Sarcoidosis were studied. The immunoperoxidase technique using monoclonal antibody to HLA-DR antigen revealed on conventional frozen sections epithelioid cell granulomas with intense staining. Localization of this HLA-DR + material on or in cells in these granulomas was possible with 1 mu semithin frozen sections. Epithelioid cells as well as multi nuclear giant cells were seen in these sections with membrane bound HLA-DR antigens. Some problems about HLA-DR antigens in relation with antigen handling, the presence on epithelioid, (and) giant cells and granuloma formation will be discussed.
D005629:Frozen Sections; D006099:Granuloma / Q000276:immunology / Q000473:pathology*; D006101:Granuloma, Giant Cell / Q000276:immunology / Q000473:pathology; D006681:HLA-D Antigens / Q000032:analysis*; D006684:HLA-DR Antigens / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D008198:Lymph Nodes / Q000032:analysis / Q000166:cytology; D012507:Sarcoidosis / Q000276:immunology / Q000473:pathology*; D013194:Staining and Labeling
D006681:HLA-D Antigens; D006684:HLA-DR Antigens