title: Differential accumulation of poly(A)+ RNA between virulent and double-stranded RNA-induced hypovirulent strains of Cryphonectria (Endothia) parasitica.
abstract: The double-stranded RNA responsible for transmissible hypovirulence in Cryphonectria (Endothia) parasitica was found to affect the accumulation of specific poly(A)+ RNA. Using differential hybridization techniques, two genes were isolated, Vir1 and Vir2, which were specifically expressed as poly(A)+ RNAs in the virulent cells. The highly expressed RNA sequences from these genes were not found in total RNA isolated from either American or European hypovirulent strains, although the genes were present in their genomes. Other virulence- and hypovirulence-specific RNA sequences were also detected. One isolated hypovirulence-specific RNA sequence was expressed in both virulent and hypovirulent cells, but in a two- to fourfold-higher concentration in the hypovirulent cells. The results show that hypovirulence is associated with concurrent changes in a few highly expressed poly(A)+ RNAs, which suggests a specific effect of the double-stranded RNA on fungal gene expression.
mesh_terms: D004247:DNA / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D005658:Fungi / Q000235:genetics / Q000472:pathogenicity*; D005796:Genes; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000235:genetics; D012313:RNA / Q000235:genetics; D012330:RNA, Double-Stranded / Q000235:genetics*; D012331:RNA, Fungal / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*
chemical_list: D004271:DNA, Fungal; D012330:RNA, Double-Stranded; D012331:RNA, Fungal; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA