title: [Antigenic polysaccharides of bacteria. 16. The structure of O-specific polysaccharide chain of Pseudomonas aeruginosa 025 (Wokatsch) lipopolysaccharide].
abstract: O-Specific polysaccharide built up of trisaccharide repeating units containing 3-acetamidino-2-acetamido-2,3-dideoxy-D-mannuronic acid (ManNAcAmA), 2,3-diacetamido-2,3-dideoxy-D-mannuronic acid (Man(NAc)2A), N-acetyl-D-fucosamine (FucNAc), and O-acetyl group was obtained on mild acid hydrolysis of P. aeruginosa O25 (Wokatsch classification) lipopolysaccharide. Basing on de-O-acetylation of polysaccharide with aqueous triethylamine accompanied by hydrolysis of acetamidino group to acetamido group, as well as on the 1H and 13C NMR data, the following structure of the repeating unit of the polysaccharide was established: (Formula: see text) P. aeruginosa O25 polysaccharide has the same carbohydrate skeleton as that of P. aeruginosa O3a,b (Lányi classification) and differs from the latter only by the presence of the O-acetyl group at position 4 of N-acetylfucosamine.
mesh_terms: D000942:Antigens, Bacterial / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D008070:Lipopolysaccharides / Q000032:analysis* / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy; D019081:O Antigens; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000276:immunology*; D012703:Serotyping
chemical_list: D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D019081:O Antigens
vernacular_title: Antigennye polisakharidy bakteriĭ. 16. Struktura O-spetsificheskoĭ polisakharidnoĭ tsepi lipopolisakharida Pseudomonas aeruginosa 025 (Wokatsch).