3-Hydroxy-3-methylglutaric, adipic, and 2-oxoglutaric acids measured by HPLC in the plasma from diabetic patients.
A method for the measurement of organic acids in human plasma is presented. The analytical procedure consists of plasma protein precipitation with acetonitrile, acid extraction by chromatography through a DEAE-cellulose column eluted with 100 mM perchloric acid, HPLC by cation-exchange column Aminex HPX-87 eluted with 6.5 mM sulfuric acid. Adipic, 3-hydroxy-3-methylglutaric, 2-oxoglutaric, and citric acids were determined in the plasma of diabetic patients. The concentrations of all the measured acids, but particularly those of adipic and 3-hydroxy-3-methylglutaric acids, were significantly higher than those of healthy controls. These results suggest that in diabetics the omega-oxidation of fatty acids is enhanced.
D000272:Adipates / Q000097:blood*; D000328:Adult; D002851:Chromatography, High Pressure Liquid; D003920:Diabetes Mellitus / Q000097:blood*; D005260:Female; D005977:Glutarates / Q000097:blood*; D006801:Humans; D007656:Ketoglutaric Acids / Q000097:blood*; D008297:Male; D015093:Meglutol / Q000097:blood*; D008875:Middle Aged
D000272:Adipates; D005977:Glutarates; D007656:Ketoglutaric Acids; D015093:Meglutol