Effects of trichostatins on differentiation of murine erythroleukemia cells.
The fungistatic antibiotics trichostatins (TS) A and C were isolated from culture broth of Streptomyces platensis No. 145 and were found to be potent inducers of differentiation in murine erythroleukemia (Friend and RV133) cells at concentrations of 1.5 X 10(-8) M for TSA and 5 X 10(-7) M for TSC. Differentiation induced by TS was cooperatively enhanced by UV irradiation but not by treatment with dimethyl sulfoxide. This enhanced activity was completely inhibited by adding cycloheximide to the culture medium 2 h after exposure to TS, suggesting that TS are dimethyl sulfoxide-type inducers of erythroid differentiation. No inhibitory effect of TS was observed on macromolecular synthesis in cultured cells.
D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects / Q000528:radiation effects; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D004261:DNA Replication / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005622:Friend murine leukemia virus; D005960:Glucosides / Q000494:pharmacology; D006877:Hydroxamic Acids / Q000494:pharmacology; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology*; D007942:Leukemia, Experimental / Q000473:pathology*; D051379:Mice; D012313:RNA / Q000096:biosynthesis; D014466:Ultraviolet Rays
D000935:Antifungal Agents; D005960:Glucosides; D006877:Hydroxamic Acids; C012589:trichostatin A; D012313:RNA; C053043:trichostatin C; D003513:Cycloheximide; D004121:Dimethyl Sulfoxide