The interaction with PDGF as a source of G1 phase heterogeneity in normal mouse fibroblasts.
In normal mouse fibroblasts exponentially growing at suboptimal serum concentration the population of cells having a presynthetic (G1) DNA content is heterogeneous, since there are present both competent and non-competent cells (Sturani et al, 1984). We show here that SV40 transformed 3T3 fibroblasts, which have lost growth control and serum requirement, lack the G1 non-competent phase. These findings are confirmed by the different kinetics of exit from the G1 compartment of transformed and normal cells. In addition a positive correlation is reported between the fraction of non-competent cells and the average abundance of the phosphorylable PDGF receptor on the cell surface of normal cells.
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects*; D002472:Cell Transformation, Viral; D003470:Culture Media; D005347:Fibroblasts / Q000166:cytology*; D007399:Interphase; D051379:Mice; D010766:Phosphorylation; D019000:Phosphotyrosine; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D011956:Receptors, Cell Surface / Q000502:physiology*; D017479:Receptors, Platelet-Derived Growth Factor; D013539:Simian virus 40; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
D003470:Culture Media; D010982:Platelet-Derived Growth Factor; D011956:Receptors, Cell Surface; D019000:Phosphotyrosine; D014443:Tyrosine; D017479:Receptors, Platelet-Derived Growth Factor