B-cells purified using azide give different responses in vitro to B-cells purified without azide.
Some established techniques for B-cell enrichment include azide in the media. Here, B-cells were enriched using a technique which transiently exposed the cells of 15 mM sodium azide. After 3-4 days in culture, the precursor frequency of autoantibody-secreting cells in this population was 0.113%. In contrast, when B-cells were enriched using a technique which eliminated their exposure to sodium azide, the number of autoantibody-secreting precursor cells detected in cultures was increased up to 20-fold. Therefore, it is concluded that transient exposure to azide affects functional activities of B-cells in vitro.
D000818:Animals; D001323:Autoantibodies / Q000378:metabolism*; D001386:Azides / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002469:Cell Separation / Q000379:methods*; D016264:Dextran Sulfate; D003911:Dextrans / Q000494:pharmacology; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D019810:Sodium Azide
D001323:Autoantibodies; D001386:Azides; D003911:Dextrans; D008070:Lipopolysaccharides; D016264:Dextran Sulfate; D019810:Sodium Azide