Comparison of angiotensin II staining in rat brain using affinity purified and crude antisera.
The use of affinity purified ANG II antiserum as opposed to crude antiserum greatly enhanced the staining resolution in rat brain. This improved resolution was due to a complete loss of background staining and an apparent increase in specific staining that was totally blockable by preabsorption. With the purified antibody it was easily possible to visualise the finest fibres in rats not treated with colchicine. Furthermore, the improved technique permitted a clearer visualisation of an ANG II-like immunoreactive product in cell bodies. This use of affinity purified antibody should greatly facilitate the mapping of central angiotensinergic pathways.
D000804:Angiotensin II / Q000032:analysis*; D000818:Animals; D001923:Brain Chemistry; D002846:Chromatography, Affinity; D006651:Histocytochemistry; D007031:Hypothalamus / Q000032:analysis; D007106:Immune Sera / Q000302:isolation & purification; D007124:Immunoenzyme Techniques; D008297:Male; D051381:Rats; D011921:Rats, Inbred WKY; D013194:Staining and Labeling
D007106:Immune Sera; D000804:Angiotensin II