Purification and partial characterization of fimbriae of Vibrio cholerae O1.
Fimbriae of Vibrio cholerae O1 were purified from a strain of the classical biotype, Inaba serotype (Bgd 17), and a strain of the El Tor biotype, Ogawa serotype (K23), grown on TCG agar medium by the following procedure; homogenization of the cell suspension to detach fimbriae, ultracentrifugation to remove remaining cells and their debris, concentration of the supernatant containing fimbriae with ultrafiltration, and 20 to 40% sucrose linear gradient centrifugation of the concentrated material. The fimbriae in both preparations were flexible, long fibres readily distinguishable under the electron microscope from those of CFA/I, CFA/II seen in ETEC strains. Their structural subunit was a protein of 16 kdaltons. Fimbriae isolated from both serotypes and biotypes shared antigenic determinants.
D000942:Antigens, Bacterial / Q000032:analysis; D000939:Epitopes / Q000032:analysis; D010861:Fimbriae, Bacterial / Q000648:ultrastructure*; D006384:Hemagglutination; D006358:Hot Temperature; D006801:Humans; D008854:Microscopy, Electron; D009439:Neuraminidase / Q000378:metabolism; D014734:Vibrio cholerae / Q000302:isolation & purification* / Q000648:ultrastructure
D000942:Antigens, Bacterial; D000939:Epitopes; D009439:Neuraminidase