title: IgG autoantibody to IgE in atopic patients.
abstract: An IgG type of antibody directed against IgE has been studied in serum from healthy and allergic individuals. The technique used is based on a solid phase paper radioimmunoassay in which the discs were sensitized with purified IgE myeloma. After incubation with patients' serum, human IgG labeled with iodine 125 was added. The anti-IgE antibodies were partially blocked by endogenous IgE in the serum and heating the serum samples at 56 degrees C disrupted the immune complexes (ie, IgG-aIgE:IgE), thereby increasing the detectable levels of IgG anti-IgE. The specificity of anti-IgE autoantibody was confirmed by both competitive inhibition and absorption experiments, using IgG, IgM, IgA, IgE, and rabbit anti-human IgG. Significantly raised levels of anti-IgE autoantibody were found in patients suffering from atopic disorders in comparison to the controls. These observations may suggest that the anti-IgE autoantibody could play a certain role in the modulation of IgE-mediated immune system and the pathogenesis of atopic diseases.
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000485:Allergens / Q000276:immunology; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis; D000918:Antibody Specificity; D001323:Autoantibodies / Q000032:analysis*; D000939:Epitopes / Q000276:immunology; D005260:Female; D006358:Hot Temperature; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D007073:Immunoglobulin E / Q000032:analysis* / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D011852:Radioallergosorbent Test / Q000379:methods; D011863:Radioimmunoassay / Q000379:methods
chemical_list: D000485:Allergens; D000888:Antibodies, Anti-Idiotypic; D001323:Autoantibodies; D000939:Epitopes; D007074:Immunoglobulin G; C026408:anti-IgE antibodies; D007073:Immunoglobulin E