Alteration of antigenic expression of human myeloid precursor cells (CFU-GM) in long-term cultures.
Comparison of myelopoiesis in continuous human bone marrow and cord blood cultures was performed. The presence of major histocompatibility complex (MHC) antigens on myeloid precursors (CFU-GM) from cord blood was confirmed using monoclonal antibodies (MoAb) defining class I (i.e., anti-beta 2-microglobulin (B2, MG1) anti-HLA-A,B heavy chain (W6/32), and anti-HLA locus B(4E), and class II (HLA-DR (7,2] antigens. The effect of long-term culture on the expression of these antigens was investigated. Our studies indicate that following prolonged in vitro culture there is a decrease in the expression of MHC antigens on CFU-GM. Our observations provide a rationale for considering the use of cord blood leukocytes subjected to long-term culture as an alternative to allogeneic hematopoietic cell transplantation.
D000375:Aging; D000911:Antibodies, Monoclonal / Q000276:immunology; D001854:Bone Marrow Cells; D002448:Cell Adhesion; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes / Q000276:immunology*; D005312:Fetal Blood / Q000166:cytology; D006098:Granulocytes / Q000166:cytology; D006680:HLA Antigens / Q000276:immunology; D006681:HLA-D Antigens / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D008264:Macrophages / Q000166:cytology; D008285:Major Histocompatibility Complex
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006680:HLA Antigens; D006681:HLA-D Antigens; D000949:Histocompatibility Antigens Class II