Localization of small RNAs hybridizable to a B2 clone in the nuclear fraction of mouse cell lines.
An RNA polymerase III transcript, 7SK nuclear RNA, was found to bear sequence homology to the B2 class of highly repeated elements in the mouse genome. Northern blot hybridizations between the small RNAs and two B2 clones showed that only one of them (p49C8) hybridized to 7SK RNA. Both clones, however, hybridized to 4.5SI RNA as well as to a third class of nuclear RNA transcripts around 170 nucleotides long, whose levels were found to be greatly increased upon induction of transformation in a mouse 3T3 cell line transformed with a temperature-sensitive mutant of simian virus 40.
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000032:analysis*; D002472:Cell Transformation, Viral; D004274:DNA, Recombinant; D051379:Mice; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000032:analysis; D012313:RNA / Q000032:analysis; D012320:RNA Polymerase III / Q000378:metabolism; D012333:RNA, Messenger; D012342:RNA, Small Nuclear / Q000032:analysis*; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D013539:Simian virus 40; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D012333:RNA, Messenger; D012342:RNA, Small Nuclear; D011061:Poly A; D012313:RNA; D012320:RNA Polymerase III