The epitope of mouse embryonic antigen(s) recognized by monoclonal antibody TEC-02 is a carbohydrate carried by high-molecular-weight glycoconjugates.
The expression and properties of mouse embryonic antigens, recognized by monoclonal antibody TEC-02, were analyzed in teratocarcinoma-derived cell lines. TEC-2 antigens were found in the majority of the parietal endoderm cells PYS-2 and in a fraction of cultured embryonal carcinoma cells but not in other cell lines tested. During the course of retinoic acid-induced differentiation of embryonal carcinoma cells F9, the expression of TEC-2 was transiently increased. Immunolabeling of extracts from F9 and PYS-2 cells separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that TEC-2 antigens are polydisperse glycoconjugates of high molecular weight (mostly greater than 100,000). The TEC-2 epitope was shown to be carbohydrate which in F9 cells might be attached to the same carrier as another developmentally regulated carbohydrate epitope TEC-1. The TEC-2 antigens, isolated by indirect immunoprecipitation, were degraded by extensive pronase digestion or mild alkaline treatment to mostly large products. Immunostaining of glycolipid standards suggested that TEC-2 epitope involves the GalNAc beta 1----4Gal beta 1----4R sequence. Combined data indicate that TEC-2 is a new developmentally regulated carbohydrate epitope carried in embryonal carcinoma cells predominantly on glycoprotein-bound large carbohydrates.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D002241:Carbohydrates / Q000276:immunology; D002272:Carcinoembryonic Antigen / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D002460:Cell Line; D002850:Chromatography, Gel; D003902:Detergents / Q000494:pharmacology; D000939:Epitopes / Q000032:analysis* / Q000276:immunology; D006001:Glycoconjugates / Q000276:immunology*; D051379:Mice; D008970:Molecular Weight; D011233:Precipitin Tests; D013724:Teratoma / Q000276:immunology; D014212:Tretinoin / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D002241:Carbohydrates; D002272:Carcinoembryonic Antigen; D003902:Detergents; D000939:Epitopes; D006001:Glycoconjugates; D014212:Tretinoin