A study of amino acid-activated currents recorded from frog motoneurones in vitro.
Superfusion of the excitatory amino acids glutamate (1-2 mM), quisqualate (15-30 microM) and N-methyl-D-aspartate (NMDA: 15-30 microM) induced inward currents in voltage-clamped motoneurones, in vitro. Typically the NMDA and quisqualate currents had prolonged time courses relative to glutamate currents. No desensitization was apparent during repeated agonist application. D-2-Amino-5-phosphonovalerate (10 microM) selectively antagonized the NMDA current without affecting the quisqualate current; the glutamate current was partially reduced reflecting its mixed agonist properties.
D015763:2-Amino-5-phosphonovalerate; D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D000870:Anterior Horn Cells / Q000187:drug effects*; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D005971:Glutamates / Q000494:pharmacology; D018698:Glutamic Acid; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects*; D008564:Membrane Potentials / Q000187:drug effects; D009046:Motor Neurons / Q000187:drug effects*; D016202:N-Methylaspartate; D009431:Neural Conduction / Q000187:drug effects; D010069:Oxadiazoles / Q000494:pharmacology; D016318:Quisqualic Acid; D011896:Rana temporaria; D014633:Valine / Q000031:analogs & derivatives / Q000494:pharmacology
D000596:Amino Acids; D005971:Glutamates; D007473:Ion Channels; D010069:Oxadiazoles; D001224:Aspartic Acid; D018698:Glutamic Acid; D016202:N-Methylaspartate; D015763:2-Amino-5-phosphonovalerate; D016318:Quisqualic Acid; D014633:Valine