Structure and expression of ubiquitin genes in higher plants.
cDNA clones encoding ubiquitin were isolated from a barley leaf cDNA library using a mammalian ubiquitin cDNA clone as probe. The nucleotide sequence of one of the clones codes for 2.2 perfect repeats of the 76-amino-acid-long ubiquitin protein with an extra lysine residue at the C-terminus. The barley ubiquitin amino acid sequence differs from the animal sequence at three positions and from the yeast sequence at two positions. The ubiquitin poly(precursor) are coded by a multigene family with 8-10 genes that produce four or five different size messengers between 700 and 2000 nucleotides in length. The large poly(A)-rich RNAs are constitutively expressed in vegetative tissues whereas the 700-nucleotide messenger(s) were only detected in tissues containing dividing cells.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000302:isolation & purification; D005796:Genes; D006609:High Mobility Group Proteins / Q000235:genetics*; D001467:Hordeum; D006801:Humans; D009693:Nucleic Acid Hybridization; D010944:Plants / Q000032:analysis / Q000235:genetics*; D011061:Poly A / Q000032:analysis; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger; D012689:Sequence Homology, Nucleic Acid; D014452:Ubiquitins / Q000032:analysis / Q000235:genetics*
D006609:High Mobility Group Proteins; D012333:RNA, Messenger; D014452:Ubiquitins; D011061:Poly A; D012313:RNA; D004247:DNA