Passive transfer of experimental allergic encephalomyelitis by myelin basic protein-specific L3T4+ T cell clones possessing several functions.
Mouse myelin basic protein (mBP)-specific T cell clones were generated from lines established from SJL/J mice immunized with mBP in complete Freund's adjuvant. These clones proliferated specifically to mBP and were propagated weekly with the same antigen for up to 8 mo. It is of particular interest that four of these phenotypic T helper clones were able to induce several T cell functions, including that of antibody production. These mBP-reactive T cell clones induced inflammatory infiltrations of the white matter of the central nervous system when transferred i.v. to irradiated (350 R) syngeneic naive recipients in concentrations as low as 0.5 X 10(6) cells/mouse. Lesions characteristic of experimental allergic encephalomyelitis (EAE) were observed as early as 5 days after transfer in the absence of clinical paralysis. Encephalitogenic clones, when added in vitro to a population of mBP-primed B cells in the presence of antigen, induced the production of anti-mBP antibodies determined by ELISA. In addition, the same clones, when transferred i.v., were found to mediate in vivo helper activity by inducing serum anti-mBP antibodies in the recipients. This response was delayed until 20 days after transfer and was abrogated by irradiation of the clones before injection. Finally, these mBP-specific specific clones were capable of mediating a specific delayed-type hypersensitivity (DTH) response. Although all four clones generated displayed the Thy-1.2+, L3T4+, Lyt-2- phenotype and proliferated specifically to mBP, only three were able to induce EAE, transfer DTH, and mediate helper activity.
D000818:Animals; D000917:Antibody Formation; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface / Q000032:analysis; D001323:Autoantibodies / Q000096:biosynthesis; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology*; D005260:Female; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007116:Immunization, Passive; D008211:Lymphocyte Cooperation; D051379:Mice; D004676:Myelin Basic Protein / Q000276:immunology*; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D001323:Autoantibodies; D004676:Myelin Basic Protein