Direct evidence for the quantum theory of the cell transformation by use of the nucleoproteide equivalents' staining.
The separation of the nucleoproteide equivalents of human Fogh and Lund [FL] cells by means of sucrose gradient centrifugation devoted 5 separate distinguished fractions. A treatment of the cells by pancreatic RNase and/or extraction of RNA by hypertonic 0.9% sodium chloride solution eliminated the last 3 fractions. An increase of the acidification of the intercalating thiazine dyes by acetic acid at 2.9 less than or equal to pH less than or equal to 5.5 accumulated the complete diffuse dispersed nucleoproteide pool in the endoplasmic reticulum and nucleus gradually over formation of chromosomal aberrations and cluster into a heap of nucleoproteide crystals with empty cytoplasmic zones.
D000650:Amnion; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure; D002471:Cell Transformation, Neoplastic; D002869:Chromosome Aberrations; D006801:Humans; D007399:Interphase; D009698:Nucleoproteins / Q000032:analysis*; D013194:Staining and Labeling
D009698:Nucleoproteins