Assay of membrane-associated antigens with monoclonal antibody.
Beta-galactosidase conjugated to erythrocyte vesicles was measured by enzymatic and immunologic methods. The immunoassay used a monoclonal antibody and an anti-mouse IgG antibody conjugated to alkaline phosphatase in an adaptation of the enzyme-linked immunosorbent assay. The beta-galactosidase and alkaline phosphatase activities were compared by correlation. The significant correlation indicates that this method is satisfactory for relative quantitation of membrane-associated antigens with exposed epitopes.
D000469:Alkaline Phosphatase / Q000378:metabolism; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D000939:Epitopes / Q000032:analysis; D004910:Erythrocyte Membrane / Q000201:enzymology / Q000276:immunology*; D012995:Solubility; D001616:beta-Galactosidase / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000939:Epitopes; D000469:Alkaline Phosphatase; D001616:beta-Galactosidase