Dynamic changes in the epidermal OKT6 positive cells at mild irritant reactions in human skin.
In the present study we induced mild irritant contact reactions by using 0.5% sodium lauryl sulphate (SLS) in distilled water or with distilled water in patch tests for 6 or 24 hours. The biopsies were taken at 6, 24, 48 and 96 hours. Light and electron microscopy were used to assess the irritant reactions produced and the monoclonal antibody OKT6 was used for the detection of the LCs. The number of the epidermal OKT6 positive dendritic cells was found to be increased at 48 and 96 hours after the exposure to SLS and at 96 hours in the water patch tests. It is concluded that mild irritant stimuli cause an increase in the LCs (OKT6 positive cells) and thus might influence and modulate the response to subsequent exposures to allergens.
D000328:Adult; D000911:Antibodies, Monoclonal; D001706:Biopsy; D003877:Dermatitis, Contact / Q000473:pathology*; D004817:Epidermis / Q000473:pathology*; D005260:Female; D006801:Humans; D007801:Langerhans Cells / Q000473:pathology; D008297:Male; D008854:Microscopy, Electron; D010328:Patch Tests; D012967:Sodium Dodecyl Sulfate; D014867:Water
D000911:Antibodies, Monoclonal; D014867:Water; D012967:Sodium Dodecyl Sulfate