Enhancing effect of absorption promoters on percutaneous absorption of a model dye (6-carboxyfluorescein) as a poorly absorbable drug. III. Histological study after addition of various absorption promoters in rats.
We have studied the effect of various absorption promoters on the percutaneous absorption dynamics of 6-carboxyfluorescein (CF) which is a poorly absorbable drug. The absorption of CF was determined by fluorescence photographic image since CF emits a strong light yellow fluorescence. The co-administration of sodium dodecylsulfate (SDS) and 2-mercaptoethanol (MER), the pretreatment with calcium thioglycolate and the co-administration of Azone (AZ) and surfactant (HCO-60) were used to promote the absorption of CF. We have also studied the injury of skin tissue by the absorption promoters. The fluorescence photographic image of rat skin, after the co-administration of SDS and MER and after the co-administration of AZ and HCO-60 were similar to the image with the stratum corneum removed from the skin. These absorption promoters did not affect the histological nature of the rat skin tissue and the recovery experiment showed no injurious effect by these absorption promoters to the skin tissue.
D000818:Animals; D001381:Azepines / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005452:Fluoresceins / Q000097:blood; D006651:Histocytochemistry; D066298:In Vitro Techniques; D007633:Keratins / Q000032:analysis; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000187:drug effects* / Q000473:pathology; D012869:Skin Absorption / Q000187:drug effects*; D013438:Sulfhydryl Compounds / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology
D001381:Azepines; D005452:Fluoresceins; D013438:Sulfhydryl Compounds; D013501:Surface-Active Agents; C035229:laurocapram; C024098:6-carboxyfluorescein; D007633:Keratins