title: Monoclonal antibody staining and impact of surgical technique on the identification and quantitation of steroid receptors in human breast cancer.
abstract: In this prospective study of 31 patients, paired receptor levels from biopsy and mastectomy specimens were found to be significantly correlated on biochemical assay. Despite this fact, 6 of 20 tumors that were estrogen receptor-positive on biopsy, had a negative assay from the mastectomy specimen, indicating the need for a fresh tumor specimen. Immunocytochemical staining with monoclonal antibody H222 Spy, an antibody specific for the estrogen receptor, was evaluated in 34 biopsy and mastectomy specimens. With a sensitivity of 85 percent and specificity of 93 percent, this technique compares favorably with the standard biochemical assay.
mesh_terms: D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D001707:Biopsy, Needle; D001943:Breast Neoplasms / Q000032:analysis* / Q000601:surgery; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000601:surgery; D004967:Estrogens / Q000032:analysis; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D008408:Mastectomy; D008875:Middle Aged; D011374:Progesterone / Q000032:analysis; D011379:Prognosis; D011446:Prospective Studies; D011960:Receptors, Estrogen / Q000032:analysis*; D011980:Receptors, Progesterone / Q000032:analysis*; D013194:Staining and Labeling
chemical_list: D000911:Antibodies, Monoclonal; D004967:Estrogens; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D011374:Progesterone