Selective rearrangement of cytokeratin filaments in cultured liver epithelial cells induced by nickel.
Rearrangements of intermediate filaments (IFs) in the liver cells involve cytokeratins, i.e., Mallory body formation. However, rearrangements of IFs observed in cell culture characteristically involve vimentin or both vimentin and cytokeratin. We report here that nickel treatment of a liver epithelial cell line (T51B) in vitro selectively induced cytokeratin filaments to accumulate in a juxtanuclear location as the cells rounded up. After withdrawal of nickel from the culture medium, vimentin filaments remained attached to the cell periphery as the cells spread out again, but the cytokeratin filaments remained aggregated in a perinuclear position without reestablishing all peripheral connections. Thus, the rearrangement of cytokeratin filaments involved partial loss of the peripheral attachments in this model.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000648:ultrastructure*; D004848:Epithelium / Q000378:metabolism / Q000648:ultrastructure; D007382:Intermediate Filaments / Q000378:metabolism / Q000648:ultrastructure*; D007633:Keratins / Q000378:metabolism*; D008099:Liver / Q000378:metabolism / Q000648:ultrastructure*; D009532:Nickel / Q000633:toxicity*; D051381:Rats; D014746:Vimentin / Q000378:metabolism
D014746:Vimentin; D007633:Keratins; D009532:Nickel