Silver staining of the nucleolar organizer regions (NORs) in semithin Lowicryl sections.
The one-step silver technique was applied to semithin Lowicryl sections of root meristem cells of Allium cepa and a human tumor cell line (TG cells). In vegetal cells, after 5 min of staining reaction, the Ag-NOR proteins formed ring-shaped structures peripherally within the nucleolus. In animal cells silver granules were distributed over the entire nucleolus. The specificity of the staining reaction was increased by incubation of the sections in NH4Cl and Schiff's reagent prior to Ag-NOR silver staining.
D000490:Allium; D000643:Ammonium Chloride; D005404:Fixatives; D006801:Humans; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D010944:Plants / Q000648:ultrastructure; D012835:Silver Nitrate; D013194:Staining and Labeling / Q000379:methods*; D013438:Sulfhydryl Compounds; D014407:Tumor Cells, Cultured
D005404:Fixatives; D013438:Sulfhydryl Compounds; D000643:Ammonium Chloride; D012835:Silver Nitrate; C005732:thioacetic acid