Effects of sialagogues on the syntheses of polyamines and DNA in murine parotid gland.
When rat parotid explants were cultured on siliconized lens paper floating on chemically defined 199 medium, all of sialagogues tested increased ornithine decarboxylase activity, which was roughly proportional to the amylase released into the culture medium. S-Adenosylmethionine decarboxylase and DNA synthesis were also induced by isoproterenol, methoxamine, carbachol and pilocarpine, but not by serotonin or substance P. The increases of the two decarboxylase activities and DNA synthesis were observed in vivo in mouse parotid gland after repeated injections of carbachol or pilocarpine. These results indicate that both adrenergic and cholinergic sialagogues stimulate the syntheses of polyamines and DNA in murine parotid gland.
D012437:Adenosylmethionine Decarboxylase / Q000378:metabolism*; D000681:Amylases / Q000378:metabolism; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D002262:Carboxy-Lyases / Q000378:metabolism*; D004261:DNA Replication / Q000187:drug effects*; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D008729:Methoxamine / Q000494:pharmacology; D051379:Mice; D009924:Organ Culture Techniques; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D010306:Parotid Gland / Q000187:drug effects / Q000378:metabolism*; D010862:Pilocarpine / Q000494:pharmacology; D011073:Polyamines / Q000378:metabolism*; D051381:Rats; D012463:Saliva / Q000378:metabolism*; D012701:Serotonin / Q000494:pharmacology; D013268:Stimulation, Chemical; D013373:Substance P / Q000494:pharmacology
D011073:Polyamines; D010862:Pilocarpine; D012701:Serotonin; D013373:Substance P; D002217:Carbachol; D000681:Amylases; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase; D012437:Adenosylmethionine Decarboxylase; D008729:Methoxamine; D007545:Isoproterenol