In vivo labelling and axonal transport of monoamine oxidase in the rat basal ganglia using radioactive pargyline.
The enzyme monoamine oxidase was labelled in the rat striatum or substantia nigra with locally injected radioactive pargyline. The binding was prevented by a pretreatment with non-radioactive pargyline, or with a combination of clorgyline and deprenyl. Most of the MAO labelled with 3H-pargyline was of the B-type, but also some MAO-A was labelled, as shown in rats pretreated with clorgyline or deprenyl separately. Seven days after the injection of (3H)-pargyline into the striatum a significant labelling was observed in the substantia nigra. This labelling was clorgyline sensitive, indicating type A MAO, and was not present when striatal neurons were destroyed with kainic acid. Labelling of the striatum following 3H-pargyline injection into the substantia nigra was also less in kainate intoxicated striata. Damage of nigral dopamine neurons with 6-hydroxydopamine did not influence the distribution of the label. Thus by using 3H-pargyline, specific labelling and axonal transport of type A MAO in striatal neurons projecting to the substantia nigra was demonstrated.
D000818:Animals; D001370:Axonal Transport / Q000187:drug effects; D001479:Basal Ganglia / Q000201:enzymology*; D003010:Clorgyline / Q000494:pharmacology; D003342:Corpus Striatum / Q000201:enzymology; D005260:Female; D006892:Hydroxydopamines / Q000494:pharmacology; D007608:Kainic Acid; D008297:Male; D008995:Monoamine Oxidase / Q000378:metabolism* / Q000502:physiology; D016627:Oxidopamine; D010293:Pargyline; D011292:Premedication; D051381:Rats; D011919:Rats, Inbred Strains; D012642:Selegiline / Q000494:pharmacology; D013378:Substantia Nigra / Q000187:drug effects
D006892:Hydroxydopamines; D012642:Selegiline; D016627:Oxidopamine; D010293:Pargyline; D008995:Monoamine Oxidase; D003010:Clorgyline; D007608:Kainic Acid