A human monoclonal IgM kappa cold agglutinin recognizing oligosaccharides with immunodominant sialyl groups preferentially at the blood group M-specific peptide backbone of glycophorins: anti-PrM.
A kappa-monotypic IgM high titer cold agglutinin reacting like anti-Pr at low, like anti-M at higher temperatures, is described. It recognizes tetra- and/or trisaccharides with immunodominant sialyl groups on glycophorins A, B, C like anti-Pr. Its affinity to the oligosaccharides is, however, approximately 10-fold increased when they are attached to the M-specific peptide backbone of glycophorin A. The antibody, termed anti-PrM, occurred in a blood group MN patient with chronic cold agglutinin disease and caused autoimmune hemolytic anemia.
D000373:Agglutinins / Q000276:immunology / Q000302:isolation & purification*; D000744:Anemia, Hemolytic, Autoimmune / Q000097:blood* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000918:Antibody Specificity; D003450:Cryoglobulins; D000939:Epitopes / Q000276:immunology*; D005260:Female; D006021:Glycophorins / Q000276:immunology*; D006386:Hemagglutination Tests; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology / Q000302:isolation & purification*; D007145:Immunoglobulin kappa-Chains / Q000276:immunology; D008951:MNSs Blood-Group System / Q000276:immunology*; D008875:Middle Aged; D019158:N-Acetylneuraminic Acid; D009844:Oligosaccharides / Q000276:immunology*; D012794:Sialic Acids / Q000276:immunology; D012795:Sialoglycoproteins / Q000276:immunology*; D013696:Temperature
D000373:Agglutinins; D000911:Antibodies, Monoclonal; D003450:Cryoglobulins; D000939:Epitopes; D006021:Glycophorins; D007075:Immunoglobulin M; D007145:Immunoglobulin kappa-Chains; D008951:MNSs Blood-Group System; D009844:Oligosaccharides; D012794:Sialic Acids; D012795:Sialoglycoproteins; C029443:cold agglutinins; D019158:N-Acetylneuraminic Acid