Unrelated peptide immunoreactivities coexist in neurons of the rat lateral dorsal hypothalamus: human growth hormone-releasing factor1-37-, salmon melanin-concentrating hormone- and alpha-melanotropin-like substances.
Antisera raised against 3 unrelated synthetic neuropeptides - salmon melanin-concentrating hormone, human growth hormone-releasing factor1-37, and alpha-melanotropin - stained the same extensive neuron population in lateral and dorsal areas of the posterior hypothalamus. Controls for specificity have shown that these 3 antisera bind 3 different epitopes. Differences in intracellular staining patterns suggest that these epitopes could be borne by distinct peptides.
D000818:Animals; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D013007:Growth Hormone-Releasing Hormone / Q000276:immunology / Q000378:metabolism*; D007028:Hypothalamic Hormones; D007031:Hypothalamus / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D008543:Melanins / Q000276:immunology / Q000378:metabolism*; D009074:Melanocyte-Stimulating Hormones / Q000276:immunology / Q000378:metabolism*; D010446:Peptide Fragments / Q000276:immunology / Q000378:metabolism*; D010907:Pituitary Hormones / Q000276:immunology / Q000378:metabolism*; D051381:Rats
D000939:Epitopes; D007028:Hypothalamic Hormones; D008543:Melanins; D010446:Peptide Fragments; D010907:Pituitary Hormones; C040787:somatotropin releasing hormone (1-37); C019463:melanin-concentrating hormone; D009074:Melanocyte-Stimulating Hormones; D013007:Growth Hormone-Releasing Hormone