Sequential G- to R-banding for high resolution chromosome analysis.
A sequential staining protocol for revealing first G- and then R-bands on early metaphase chromosomes is presented. Lymphocyte cultures are synchronized with methotrexate, and released from the S-phase block with bromodeoxyuridine. The resulting early metaphase cells are initially G-banded with Wright's stain and then R-banded by the fluorescence plus Giemsa (FPG) technique.
D002478:Cells, Cultured; D002871:Chromosome Banding / Q000379:methods*; D006801:Humans; D007621:Karyotyping; D013194:Staining and Labeling