Evidence for a complex structure of neutralization antigenic site I of poliovirus type 1 Mahoney.
We have selected neutralization escape mutants by using a monoclonal antibody (nt-MAb) against a sequential epitope between amino acids 93 through 104 (neutralization antigenic site I) of poliovirus type 1 Mahoney. The majority of mutants were also resistant against five strain-specific nt-MAbs which recognized conformation-dependent epitopes, suggesting that the neutralization antigenic site I must be involved in the formation of such epitopes. An analysis of all mutants by the binding of nt-MAbs and by isoelectric focusing of VP1 allowed discrimination of five classes of mutants. Sequence analysis of mutant RNAs revealed point mutations and deletions in the antibody-binding site.
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000235:genetics*; D001483:Base Sequence; D002872:Chromosome Deletion; D000939:Epitopes / Q000032:analysis / Q000235:genetics*; D007525:Isoelectric Focusing; D009154:Mutation; D017955:Poliovirus / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes; D012367:RNA, Viral