Short-term tests of genotoxicity for 1,1,1-trichloroethane.
Covalent binding of 14C-1,1,1-trichloroethane to macromolecules from rat and mouse liver, kidney, lung and stomach was analyzed under the same experimental conditions previously utilized in studying 1,1-dichloroethane and 1,1,2-trichloroethane. Labeling of DNA, RNA and proteins was very low both in in vivo interaction and in in vitro microsome-mediated binding. Interaction proceeded through the involvement of the P-450-dependent mixed function oxidase system from liver microsomes and, to a lesser extent, from lung microsomes. Covalent Binding Index of 1,1,1-trichloroethane in liver DNA was typical of very weak initiators. However, overall evaluation of the short-term assays available for 1,1,1-trichloroethane leads to limited evidence of genotoxicity. On the other hand, the evidence of 1,1,1-trichloroethane carcinogenicity in animals is still inadequate.
D000818:Animals; D002250:Carbon Radioisotopes; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism*; D006843:Hydrocarbons, Chlorinated / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism*; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012997:Solvents; D013270:Stomach / Q000187:drug effects; D014240:Trichloroethanes / Q000378:metabolism / Q000633:toxicity*
D002250:Carbon Radioisotopes; D006843:Hydrocarbons, Chlorinated; D011506:Proteins; D012997:Solvents; D014240:Trichloroethanes; C024566:1,1,1-trichloroethane; D012313:RNA; D004247:DNA; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases