title: Ionic channels formed by Staphylococcus aureus alpha-toxin: voltage-dependent inhibition by divalent and trivalent cations.
abstract: The interaction of Staphylococcus aureus alpha-toxin with planar lipid membranes results in the formation of ionic channels whose conductance can be directly measured in voltage-clamp experiments. Single-channel conductance depends linearly on the solution conductivity suggesting that the pores are filled with aqueous solution; a rough diameter of 11.4 +/- 0.4 A can be estimated for the pore. The conductance depends asymmetrically on voltage and it is slightly anion selective at pH 7.0, which implies that the channels are asymmetrically oriented into the bilayer and that ion motion is restricted at least in a region of the pore. The pores are usually open in a KCl solution but undergo a dose- and voltage-dependent inactivation in the presence of di- and trivalent cations, which is mediated by open-closed fluctuations at the single-channel level. Hill plots indicate that each channel can bind two to three inactivating cations. The inhibiting efficiency follows the sequence Zn2+ greater than Tb3+ greater than Ca2+ greater than Mg2+ greater than Ba2+, suggesting that carboxyl groups of the protein may be involved in the binding step. A voltage-gated inactivation mechanism is proposed which involves the binding of two polyvalent cations to the channel, one in the open and one in the closed configuration, and which can explain voltage, dose and time dependence of the inactivation.
mesh_terms: D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D002412:Cations; D004553:Electric Conductivity; D007473:Ion Channels / Q000502:physiology*; D008433:Mathematics; D008954:Models, Biological
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D002412:Cations; D007473:Ion Channels; C017724:staphylococcin