title: Isolation and characterisation of monoclonal antibodies against hydrophobic membrane subunit 9 of the yeast mitochondrial H+-ATPase.
abstract: Five stable lines of myeloma-spleen cell hybrids, producing antibodies against the proteolipid subunit 9 of the yeast mitochondrial H+-ATPase F0-sector, have been isolated by immunizing mice with a proteolipid preparation in the presence of sodium dodecyl sulphate. One of these monoclonal antibodies also reacted with subunit 8 of the enzyme complex indicating a shared epitope. The antibodies did not react with the holo-H+-ATPase, suggesting that their epitopes are shielded by other subunits of the enzyme complex.
mesh_terms: D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000946:Antigens, Fungal / Q000276:immunology; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D005656:Fungal Proteins / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008928:Mitochondria / Q000201:enzymology; D011510:Proteolipids / Q000276:immunology*; D006180:Proton-Translocating ATPases / Q000276:immunology*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000276:immunology
chemical_list: D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal; D000939:Epitopes; D005656:Fungal Proteins; D011510:Proteolipids; D006180:Proton-Translocating ATPases