The effect of cyclic AMP on the accumulation of newly synthesized protein in the extracellular matrix of PFHR-9 teratocarcinoma cells.
The effect of elevated cyclic AMP levels on the accumulation of newly synthesized extracellular matrix protein was examined in PFHR-9 cells producing Type IV collagen. The effect of dbcAMP, 8-BrcAMP, IBMX and forskolin on the synthesis of total protein, non-collagen protein and collagen were compared. DbcAMP increased the accumulation of total protein but did not affect the distribution of collagen and non-collagen protein. 8-BrcAMP, IBMX and forskolin also increased collagen and non-collagen accumulation. However, the effect on collagen was significantly greater with 8-BrcAMP and IBMX. Consequently, 8-BrcAMP and IBMX resulted in an increased percent collagen synthesis in the extracellular matrix. Elevated cAMP levels had no effect on cell proliferation or DNA synthesis but did produce a significant effect on cell morphology.
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D003994:Bucladesine / Q000494:pharmacology; D002455:Cell Division; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology; D003094:Collagen / Q000378:metabolism; D000242:Cyclic AMP / Q000502:physiology*; D004247:DNA / Q000096:biosynthesis; D005109:Extracellular Matrix / Q000378:metabolism*; D011392:Proline / Q000378:metabolism; D014176:Protein Biosynthesis; D013724:Teratoma
D005576:Colforsin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D003994:Bucladesine; D003094:Collagen; D004247:DNA; D011392:Proline; D000242:Cyclic AMP; D015056:1-Methyl-3-isobutylxanthine