Staining of retinal neurons in the isolated eyecup by extracellular horseradish peroxidase injection.
A reliable method has been developed for the staining of mammalian retinal neurons in the isolated eyecup. Small injections of horseradish peroxidase are made at a number of places on the retinal surface while the hemisected eyecup preparation floats in Eagle's medium. After 30 min, the retinas are removed, fixed, and reacted with diaminobenzidine. Golgi-like staining of fine dendrites, spines and axons of ganglion cells is achieved. The method also stains amacrine, horizontal and bipolar cells.
D000818:Animals; D002415:Cats; D003584:Cytological Techniques; D005289:Ferrets; D006735:Horseradish Peroxidase; D009474:Neurons / Q000166:cytology*; D011817:Rabbits; D012160:Retina / Q000166:cytology*; D012165:Retinal Ganglion Cells / Q000166:cytology; D013194:Staining and Labeling
D006735:Horseradish Peroxidase