Electrophoretic and immunochemical study of the lipopolysaccharides produced by chemostat-grown Escherichia coli O157.
Two chemically different O-polysaccharides, a low molecular mass form of LPS and core LPS produced by chemostat-grown E. coli O157, were analysed by SDS-PAGE, silver staining and immunoblotting. The reactivities of the different O-polysaccharides with antisera prepared against E. coli O157 grown in batch culture, Salmonella O30 or Brucella abortus were very similar, showing that the O-polysaccharides share at least some antigenic determinants. The reactions of the low molecular mass LPS with the antisera indicated it was semi-rough LPS having one repeat unit of the O-polysaccharide attached to core LPS.
D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D004926:Escherichia coli / Q000276:immunology / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000096:biosynthesis* / Q000276:immunology
D000939:Epitopes; D008070:Lipopolysaccharides