title: Comparison of a within-day solid-phase immunoradiometric assay with a solution-phase radioimmunoassay for the measurement of alpha-fetoprotein in amniotic fluid.
abstract: Using 231 amniotic fluid samples from a Regional Screening Service, the performance of a monoclonal antibody-based solid-phase immunoradiometric assay (IRMA) for alpha-fetoprotein was compared with that of a polyclonal antiserum-based solution phase radioimmunoassay (RIA). alpha-Fetoprotein values determined from these samples by the two methods were in excellent agreement (r = 0.992) and the diagnostic performance of the two assays was identical. However, the IRMA assay displayed a greater working range than the RIA, and in addition was more rapid to perform, allowing within-day turnaround of laboratory results.
mesh_terms: D000653:Amniotic Fluid / Q000032:analysis*; D005260:Female; D005865:Gestational Age; D006801:Humans; D011247:Pregnancy; D011863:Radioimmunoassay; D000509:alpha-Fetoproteins / Q000032:analysis*
chemical_list: D000509:alpha-Fetoproteins