Expression of pemphigoid antigen by SV40-transformed human keratinocytes.
The expression and properties of pemphigoid antigen of SV40-transformed human keratinocytes were studied. By indirect immunofluorescence, SV40-transformed keratinocytes in passage 80-85 expressed the pemphigoid antigen as coarsely granular perinuclear fluorescence. To characterize this antigen, NP40 extracts of cells labeled with [14C]amino acids were immunoprecipitated using sera of 8 patients: bullous pemphigoid (6 patients), chronic localized pemphigoid (1 patient), and drug-induced lichen planus pemphigoides (1 patient). These immunoprecipitates were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis and then fluorographed. All 8 sera precipitated a protein of Mr 240K, while normal human sera did not precipitate this protein. These results indicate that SV40-transformed human keratinocytes synthesize pemphigoid antigen, and that autoantibodies in the sera of pemphigoid patients with different clinical features identify the same antigen of Mr 240K in these cells.
D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000032:analysis*; D001324:Autoantigens / Q000032:analysis* / Q000096:biosynthesis; D002472:Cell Transformation, Viral; D004817:Epidermis / Q000276:immunology* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D007231:Infant, Newborn; D007633:Keratins; D008010:Lichen Planus / Q000276:immunology / Q000378:metabolism; D010391:Pemphigoid, Bullous / Q000276:immunology / Q000378:metabolism; D010392:Pemphigus / Q000276:immunology* / Q000378:metabolism; D011233:Precipitin Tests; D013539:Simian virus 40
D000941:Antigens; D001324:Autoantigens; D007633:Keratins