title: Histochemical techniques to localize rubber in guayule (Parthenium argentatum Gray).
abstract: There are few histochemical techniques for staining rubber in the cells of guayule. With regard to specificity, clarity and retention of the stain we found staining with oil red O or iodine-potassium iodide for bright field, and a combination of oil red O and dansyl chloride for epifluorescence microscopy to be the best methods. The plausible mechanisms of staining are discussed.
mesh_terms: D001391:Azo Compounds; D001576:Benzophenoneidum; D001966:Bromine; D003619:Dansyl Compounds; D007455:Iodine; D007840:Latex / Q000032:analysis*; D008856:Microscopy, Fluorescence; D009499:Neutral Red; D009993:Osmium Tetroxide; D010944:Plants / Q000032:analysis*; D011193:Potassium Iodide; D012835:Silver Nitrate; D013194:Staining and Labeling
chemical_list: D001391:Azo Compounds; D003619:Dansyl Compounds; D007840:Latex; D011193:Potassium Iodide; D001576:Benzophenoneidum; D009499:Neutral Red; D012835:Silver Nitrate; D007455:Iodine; C011049:oil red O; D009993:Osmium Tetroxide; C029038:dansyl chloride; D001966:Bromine