Identification of multiple novel polypeptide substrates of the v-src, v-yes, v-fps, v-ros, and v-erb-B oncogenic tyrosine protein kinases utilizing antisera against phosphotyrosine.
Antibodies to phosphotyrosine were used to identify proteins phosphorylated on tyrosine in chicken embryo fibroblasts transformed by the oncogenes, v-src, v-yes, v-fps, v-ros, or v-erb-B. These antibodies allowed detection of a minimum of 50, 44, and 47 bands in immunoblots of cellular lysates from fibroblasts transformed by v-src, v-yes, or v-fps respectively. Eight of these bands were also detected in fibroblasts transformed by v-ros and v-erb-B, suggesting that the cellular transformation induced by v-ros, v-erb-B, v-src, v-yes, and v-fps may be achieved by the phosphorylation of some of the same proteins. The viruses SD10 and SD11 are point mutants of the Prague-C strain of Rous sarcoma virus that encode non-myristylated p60v-src. They do not induce the transformation of chicken fibroblasts. Approximately thirty bands were detected by antibodies to phosphotyrosine in fibroblasts transformed by wild-type Prague-RSV-C, only four of which were not substrates nonmyristylated mutant p60v-src. This suggests that the phosphorylation of a large subset of the substrates of p60v-src is not sufficient for transformation.
D000818:Animals; D000906:Antibodies / Q000276:immunology; D001358:Avian Sarcoma Viruses / Q000201:enzymology; D002472:Cell Transformation, Viral; D002642:Chick Embryo; D005347:Fibroblasts / Q000201:enzymology; D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D010750:Phosphoproteins / Q000096:biosynthesis; D010766:Phosphorylation; D019000:Phosphotyrosine; D011499:Protein Processing, Post-Translational; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D013379:Substrate Specificity; D014443:Tyrosine / Q000031:analogs & derivatives / Q000276:immunology
D000906:Antibodies; D009856:Oncogene Proteins, Viral; D010750:Phosphoproteins; D019000:Phosphotyrosine; D014443:Tyrosine; D011505:Protein-Tyrosine Kinases