A qualitative and quantitative assessment of the humoral antibody response of the sheep to orf virus infection.
The serological response of naturally and experimentally infected lambs to orf virus infection was analysed using an enzyme-linked immunosorbent assay (ELISA) together with the Western blotting technique. The combination of these two methods permitted a qualitative and quantitative assessment of the response, which revealed considerable variation between animals. Despite this, all post-exposure sera reacted with a polypeptide (molecular weight 40 kilodaltons) which appears to be a component of the surface tubules that are characteristic of the virus.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D000956:Antigens, Viral / Q000276:immunology*; D004474:Ecthyma, Contagious / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D007118:Immunoassay; D007700:Kinetics; D009923:Orf virus / Q000276:immunology*; D011212:Poxviridae / Q000276:immunology*; D012756:Sheep; D013047:Specific Pathogen-Free Organisms
D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes