Analysis of alpha-fetoprotein heterogeneity by an improved crossed immunoaffinoelectrophoretic technique.
The crossed immunoaffinoelectrophoretic procedure described here for the analysis of alpha-fetoprotein heterogeneity includes three major modifications of a procedure previously used by others: impregnation of the second dimension gel with methylated sugar derivatives; rigorous gel washing at 37 degrees C with the same derivatives; and an immunostaining technique. These simple changes have resulted in clear, sharp electrophoretic patterns at levels of total alpha-fetoprotein as low as 50 ng/ml, a sensitivity not previously approached with the method. Individual peaks representing as little as 7.5 ng/ml can be identified.
D003208:Concanavalin A; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D007124:Immunoenzyme Techniques; D037102:Lectins; D008832:Microchemistry; D037121:Plant Lectins; D000509:alpha-Fetoproteins / Q000032:analysis* / Q000276:immunology
D037102:Lectins; D037121:Plant Lectins; D000509:alpha-Fetoproteins; C033619:lentil lectin; D003208:Concanavalin A