Identification of Trypanosoma cruzi in human tissues using an immunoperoxidase method: study of acute Chagas disease, congenital form.
The immunoperoxidase technique using a polyclonal T. cruzi rabbit antiserum was compared with the routine hematoxylin-eosin staining procedure in the study of two cases of congenital Chagas disease. Comparatively, the immunoperoxidase technique gave better results than that using hematoxylin-eosin in identification of T. cruzi. The immunoperoxidase technique detected T. cruzi nests in unsuspected sites like lungs and testes, in which the hematoxylin-eosin stain failed to demonstrate their presence. In the two cases studied there was no evidence of positive immunoperoxidase reaction which could suggest the presence of cross-antigens between T. cruzi and host tissues, in the heart, gastrointestinal tract or other organs. These negative results are discussed.
D000818:Animals; D014355:Chagas Disease / Q000151:congenital / Q000276:immunology / Q000469:parasitology*; D003429:Cross Reactions; D004801:Eosine Yellowish-(YS); D005260:Female; D006416:Hematoxylin; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D013194:Staining and Labeling; D014349:Trypanosoma cruzi / Q000276:immunology / Q000302:isolation & purification*
D004801:Eosine Yellowish-(YS); D006416:Hematoxylin