Establishment of a rat nasal epithelial tumor cell line.
A new cell line designated NAS 2BL has been established from a rat nasal tumor induced by inhalation of the direct-acting carcinogen methylmethane sulfonate. The cells are epithelial in morphology, have a generation time of 34 h, require 10% fetal bovine serum for optimal growth, and exhibit keratinization at confluence. The karyotype is aneuploid, with several marker chromosomes, and the cells are transformed by the criterion of nude mouse tumorigenicity.
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002455:Cell Division; D002460:Cell Line; D003470:Culture Media; D004848:Epithelium; D007621:Karyotyping; D007633:Keratins; D009297:Nasal Mucosa / Q000473:pathology*; D009669:Nose Neoplasms / Q000473:pathology*; D009857:Oncogenes; D010641:Phenotype; D051381:Rats
D003470:Culture Media; D007633:Keratins