[Action of chloramine-T on the processes of sodium channel activation and inactivation in neuroblastoma cells].
Effect of chloramine-T, the specific reagent on methionine residues, on gating of sodium channels was studied in neuroblastoma cell membrane. After the chloramine treatment the inactivation became slower, incomplete and the steepness of its voltage dependence considerably decreased; the inactivation curve was shifted towards depolarization. Time course of activation did not change. The activation curve shifted towards negative potentials, its slope being insignificantly decreased. Effective charge of activation as determined from the limiting logarithmic slope of the activation decreased by a factor of 1.17. Possible explanations of the phenomena observed are discussed.
D000818:Animals; D002700:Chloramines / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007473:Ion Channels / Q000187:drug effects* / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D009447:Neuroblastoma / Q000503:physiopathology*; D012964:Sodium / Q000378:metabolism*; D013997:Time Factors; D014105:Tosyl Compounds; D014407:Tumor Cells, Cultured
D002700:Chloramines; D007202:Indicators and Reagents; D007473:Ion Channels; D014105:Tosyl Compounds; C016300:chloramine-T; D012964:Sodium
Issledovanie deĭstviia khloramina-T na protsessy aktivatsii i inaktivatsii natrievykh kanalov kletok neĭroblastomy.