A rapid methylene blue staining procedure for the ventral nerve cord of holometabolous insects.
The author describes a modification of the methylene blue technique that stains neurons dark blue against a clear background. The resolution of the dendritic tree attains Golgi-like quality. The procedure offers a rapid, easily adaptable alternative to the reduced silver procedures in holometabolous insects.
D000818:Animals; D002490:Central Nervous System / Q000033:anatomy & histology; D001517:Coleoptera / Q000033:anatomy & histology*; D007915:Lepidoptera / Q000033:anatomy & histology*; D008751:Methylene Blue; D009474:Neurons / Q000166:cytology; D013194:Staining and Labeling
D008751:Methylene Blue