Biochemical and functional characterization of a novel neuron-glia adhesion molecule that is involved in neuronal migration.
Adhesion molecule on glia (AMOG) is a novel neural cell adhesion molecule that mediates neuron-astrocyte interaction in vitro. In situ AMOG is expressed in the cerebellum by glial cells at the critical developmental stages of granule neuron migration. Granule neuron migration that is guided by surface contacts between migrating neurons and astroglial processes is inhibited by monoclonal AMOG antibody, probably by disturbing neuron-glia adhesion. AMOG is an integral cell surface glycoprotein of 45-50-kD molecular weight with a carbohydrate content of at least 30%. It does not belong to the L2/HNK-1 family of neural cell adhesion molecules but expresses another carbohydrate epitope that is shared with the adhesion molecules L1 and myelin-associated glycoprotein, but is not present on N-CAM or J1.
D000251:Adenosine Triphosphatases; D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis* / Q000276:immunology; D001253:Astrocytes / Q000737:chemistry / Q000502:physiology*; D002118:Calcium / Q000494:pharmacology; D027682:Cation Transport Proteins; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules; D015816:Cell Adhesion Molecules, Neuronal; D002465:Cell Movement; D002525:Cerebellar Cortex / Q000276:immunology; D002846:Chromatography, Affinity; D000939:Epitopes / Q000276:immunology; D016326:Extracellular Matrix Proteins; D005347:Fibroblasts / Q000737:chemistry; D007158:Immunologic Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008819:Mice, Nude; D009474:Neurons / Q000737:chemistry / Q000502:physiology*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; C504191:Atp1b2 protein, mouse; D027682:Cation Transport Proteins; D015815:Cell Adhesion Molecules; D015816:Cell Adhesion Molecules, Neuronal; D000939:Epitopes; D016326:Extracellular Matrix Proteins; D000251:Adenosine Triphosphatases; D002118:Calcium