Murine thymic lymphomas after infection with a B-ecotropic murine leukemia virus and/or X-irradiation: proviral organization and RNA expression.
The role of retroviruses in murine radioleukemogenesis was reinvestigated using a protocol associating the injection of a non-pathogenic retrovirus (T1223/B virus) and a subleukemogenic dose of X-radiation (2 X 1.75 Gy). Using the Southern blotting technique we studied MuLV proviral organization and RNA expression in thymic lymphomas induced by the combined effect of virus and irradiation or irradiation alone. A recombinant provirus was detected in the chromosomal DNA of every tumor induced by associating virus and radiation whereas it was unconstantly found in radio-induced tumors. In every instance, the provirus was not integrated at a common site. No relationship was observed between viral RNA expression and tumor induction. Trisomy 15 was observed in all metaphases irrespective of the protocol of tumor induction. The G-banding technique revealed an extra-band in several thymic lymphomas induced by irradiation and T1223/B virus injection.
D000818:Animals; D002869:Chromosome Aberrations / Q000235:genetics / Q000473:pathology; D025063:Chromosome Disorders; D004262:DNA Restriction Enzymes; D004273:DNA, Neoplasm / Q000235:genetics; D005786:Gene Expression Regulation; D005814:Genes, Viral; D007942:Leukemia, Experimental / Q000209:etiology / Q000235:genetics*; D007953:Leukemia, Radiation-Induced / Q000235:genetics* / Q000382:microbiology; D008223:Lymphoma / Q000209:etiology* / Q000235:genetics / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D011061:Poly A / Q000235:genetics; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D011995:Recombination, Genetic; D012190:Retroviridae / Q000235:genetics / Q000472:pathogenicity*; D013953:Thymus Neoplasms / Q000235:genetics* / Q000382:microbiology; D014759:Viral Envelope Proteins / Q000235:genetics; D014965:X-Rays
D004273:DNA, Neoplasm; D012333:RNA, Messenger; D012367:RNA, Viral; D014759:Viral Envelope Proteins; D011061:Poly A; D012313:RNA; D004262:DNA Restriction Enzymes