title: Effects on peritoneal proteolysis and hemodynamics of prophylactic and therapeutic infusions of high doses of aprotinin in experimental acute pancreatitis.
abstract: Acute pancreatitis was induced in pigs by retrograde injection of Na-taurocholate into the pancreatic duct. Chromogenic peptide substrate assays showed increased trypsin (TRY) and plasma kallikrein activity (KK), parallel with a reduction of plasma prekallikrein (PKK) and functional kallikrein inhibition (KKI) values, in the peritoneal exudate in untreated animals. Intravenous high-dose pretreatment or therapy with aprotinin starting 3 h after the induction of acute pancreatitis resulted in significantly increased KKI capacity and unchanged KK and TRY activities in the peritoneal exudate. In test animals receiving aprotinin intravenously a significantly increased survival rate and improved cardiac output and arterial blood pressure were found during the 6-h observation period. All animals treated with aprotinin survived the observation period, whereas 63% of the untreated animals died. The study emphasizes the pathophysiological importance of the plasma kallikrein-kinin system in acute pancreatitis.
mesh_terms: D000208:Acute Disease; D000681:Amylases / Q000097:blood; D000818:Animals; D007611:Aprotinin / Q000008:administration & dosage*; D001202:Ascitic Fluid / Q000201:enzymology*; D006439:Hemodynamics / Q000187:drug effects*; D007262:Infusions, Intravenous; D007610:Kallikreins / Q000097:blood; D007705:Kinins / Q000097:blood; D010195:Pancreatitis / Q000188:drug therapy*; D010447:Peptide Hydrolases / Q000378:metabolism*; D013552:Swine; D014357:Trypsin / Q000378:metabolism
chemical_list: D007705:Kinins; D007611:Aprotinin; D000681:Amylases; D010447:Peptide Hydrolases; D007610:Kallikreins; D014357:Trypsin