title: Spontaneous vesicularization of myelin lipids is counteracted by myelin basic protein.
abstract: Hand-vortexed dispersions of several lipids (cerebrosides, sulfatides, PC, PE, PS and sphingomyelin), mixed in the ratios found for these categories of lipids in myelin, exhibit 31P-NMR spectra which have contributions from both isotropic and lamellar resonances. Investigation of this system by freeze-fracture electron microscopy and X-ray diffraction revealed that this lipid mixture has spontaneously formed small unilamellar vesicles (SUVs) (diam. approximately 400 A) and large highly convoluted unilamellar vesicles (LUVs) (diam. approximately 1000 A), the latter possibly resulting from aggregation and fusion of the SUV structures. This vesicularization of the myelin lipids was reversed by the addition of myelin basic protein: only large multilamellar aggregates were formed in the presence of protein, as shown by all three experimental methods. Although no rigorous physical-chemical explanation for these phenomena is yet available, the possibility is suggested that the high concentration of cerebrosides and/or phosphatidylethanolamine in this particular mixture of myelin lipids play pivotal roles in the formation of these unusual vesicles. Spontaneous vesicularization of myelin lipids is discussed as a potential pathway toward destabilization of the myelin sheath.
mesh_terms: D002499:Centrifugation, Density Gradient; D002554:Cerebrosides; D005614:Freeze Fracturing; D006801:Humans; D008051:Lipid Bilayers; D046911:Macromolecular Substances; D009682:Magnetic Resonance Spectroscopy; D008563:Membrane Lipids; D008854:Microscopy, Electron; D004676:Myelin Basic Protein / Q000494:pharmacology*; D009186:Myelin Sheath / Q000032:analysis*; D010743:Phospholipids; D013109:Sphingomyelins; D013433:Sulfoglycosphingolipids; D014961:X-Ray Diffraction
chemical_list: D002554:Cerebrosides; D008051:Lipid Bilayers; D046911:Macromolecular Substances; D008563:Membrane Lipids; D004676:Myelin Basic Protein; D010743:Phospholipids; D013109:Sphingomyelins; D013433:Sulfoglycosphingolipids