title: Functional analysis of activated C1s, a subcomponent of the first component of human complement, by monoclonal antibodies.
abstract: Three mouse monoclonal antibodies (M365, M81, and M241) directed against human C1s were used to analyze the structure of C1s related to the enzymatic activity. M365 and M81 recognized different epitopes on the heavy chain of C1s and could bind to C1s, as well as to C1s. The C4 cleaving activity of C1s was completely blocked by M81 and was partially blocked by M365. Although the C2 cleaving activity of C1s was partially inhibited by M81, no blocking was observed with M365. Both antibodies had no effect on the esterolytic activity of C1s. These results indicate that the C4 and C2 binding sites on C1s reside in the heavy chain, and they are distinct from each other. M241 could bind only to C1s, an active form of C1s. After reduction of C1s, M241 could not react with either heavy or light chain of C1s. The esterolytic activity of C1s was markedly reduced by M241. Furthermore, M241 blocked not only the cleavage of C4 and C2 by C1s but also the complex formation of C1s and C1 inactivator. From these observations, we suggest that M241 reacts with the active site of C1s, and both heavy and light chains of C1s participate in the composition of the active site.
mesh_terms: D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D003166:Complement Activating Enzymes / Q000502:physiology*; D003174:Complement C1 Inactivator Proteins / Q000378:metabolism; D003173:Complement C1s; D003175:Complement C2 / Q000378:metabolism; D003181:Complement C4 / Q000378:metabolism; D000939:Epitopes; D004950:Esterases / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007700:Kinetics
chemical_list: D000911:Antibodies, Monoclonal; D003174:Complement C1 Inactivator Proteins; D003175:Complement C2; D003181:Complement C4; D000939:Epitopes; D003166:Complement Activating Enzymes; D004950:Esterases; D003173:Complement C1s