title: Centrosomal proteins and lactate dehydrogenase possess a common epitope in human cell lines.
abstract: A spontaneously arising rabbit anti-centrosome serum with strong human specificity, used to identify specific antigens in isolated centrosomes, was shown to react with several noncentrosomal proteins including a 36-kDa protein that appeared to be the major cellular antigen. To explore the immunological relationship between noncentrosomal and centrosomal antigens, immunoglobulins were affinity purified using the individual noncentrosomal antigens (from lymphoblastoma KE37 cells) and were tested for their capacity to bind to human centrosomes in situ and to proteins from isolated centrosomes. In this way, the 36-kDa antigen, an abundant cytosolic protein, was shown to share at least one antigenic determinant with high molecular weight centrosomal proteins. This antigen was further identified by mild proteolysis as the glycolytic enzyme lactate dehydrogenase. In all the analyzed human cell lines, the centrosomal staining in situ was correlated with a strong labeling of purified lactate dehydrogenase in immunoblots. Conversely, the absence of centrosomal staining in rodent cells was always correlated with the absence of lactate dehydrogenase labeling. These data suggest an evolutionary relationship between centrosomal proteins and this "housekeeping" enzyme.
mesh_terms: D000818:Animals; D002460:Cell Line; D003600:Cytosol / Q000201:enzymology / Q000276:immunology / Q000378:metabolism*; D000939:Epitopes; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000276:immunology* / Q000378:metabolism; D011506:Proteins / Q000276:immunology* / Q000378:metabolism; D012377:Rodentia
chemical_list: D000939:Epitopes; D011506:Proteins; D007770:L-Lactate Dehydrogenase