[DNA-synthesizing activity of the nucleoid protein core in Escherichia coli].
Nucleoids obtained from E. coli cells by extraction with 1 M NaCl and detergents containing solution were further extracted with 2 M NaCl. From these samples, that contain only tightly bound proteins, fractions of protein core and peripheral nucleoprotein were obtained. It is shown that DNA synthesis proceeds mainly in the core structures. We have found that DNA polymerase I, which is bound with DNA nucleoid loops and with the above mentioned core structures, is not dissociating in 2M NaCl solution.
D001426:Bacterial Proteins / Q000378:metabolism*; D002458:Cell Fractionation / Q000379:methods; D002467:Cell Nucleus / Q000378:metabolism*; D002876:Chromosomes, Bacterial / Q000378:metabolism; D004256:DNA Polymerase I / Q000378:metabolism; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004926:Escherichia coli / Q000378:metabolism* / Q000648:ultrastructure; D012329:RNA, Bacterial / Q000378:metabolism
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D004256:DNA Polymerase I
DNK-sinteziruiushchaia aktivnost' belkovogo ostova nukleoida Escherichia coli.