[Specific for apurine-apyrimidine DNA endodeoxyribonuclease from the rat brain].
Endodeoxyribonuclease was detected in rat neocortex chromatin. The partly purified enzyme was found to influence the superhelical apurine-apyrimidine DNA 50 times as effectively as compared to the native substrate. The enzyme hydrolyzes the phosphodiester bond with the formation of 3'-OH- and 5'-phosphate terminal groups. The enzyme-hydrolyzed DNA is an effective primer for DNA-polymerase I from E. coli. It was assumed that DNAase from rat brain chromatin is an apurine-apyrimidine endonuclease II.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D004256:DNA Polymerase I / Q000378:metabolism; D004260:DNA Repair; D004278:DNA, Superhelical / Q000378:metabolism; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D043223:Deoxyribonuclease IV (Phage T4-Induced); D004706:Endodeoxyribonucleases / Q000302:isolation & purification* / Q000378:metabolism; D006868:Hydrolysis; D051381:Rats; D011919:Rats, Inbred Strains
D004278:DNA, Superhelical; D004256:DNA Polymerase I; D004706:Endodeoxyribonucleases; D043223:Deoxyribonuclease IV (Phage T4-Induced); D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase
Spetsifichnaia k apurin-apirimidinovoĭ DNK éndodezoksiribonukleaza golovnogo mozga krys.