New tester strains of Salmonella typhimurium highly sensitive to mutagenic nitroarenes.
The nitroreductase and acetyltransferase genes of Salmonella typhimurium TA1538 have been cloned into pBR322. When transformed into TA1538 derivatives, these plasmids provided the basis for an extremely sensitive assay for the detection of mutagenic nitroarenes.
D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity*; D000123:Acetyltransferases / Q000235:genetics*; D003001:Cloning, Molecular; D006571:Heterocyclic Compounds / Q000633:toxicity*; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000032:analysis*; D009574:Nitro Compounds / Q000633:toxicity; D009583:Nitrofurazone / Q000633:toxicity*; D009600:Nitroquinolines / Q000633:toxicity*; D009601:Nitroreductases / Q000235:genetics*; D010088:Oxidoreductases / Q000235:genetics*; D011083:Polycyclic Compounds / Q000633:toxicity*; D011721:Pyrenes / Q000633:toxicity*; D012486:Salmonella typhimurium / Q000187:drug effects*
D006571:Heterocyclic Compounds; D009153:Mutagens; D009574:Nitro Compounds; D009600:Nitroquinolines; D011083:Polycyclic Compounds; D011721:Pyrenes; C033310:1,8-dinitropyrene; D015112:4-Nitroquinoline-1-oxide; D010088:Oxidoreductases; D009601:Nitroreductases; D000123:Acetyltransferases; C032668:1-nitropyrene; D009583:Nitrofurazone