DNA synthesis induced by the neuropeptide substance K correlates to the level of myc-gene transcripts.
Neuropeptides have recently been implicated in regulation of normal and neoplastic cell growth. Substance K is a neurotransmittor candidate that has been identified as a mitogen for smooth muscle cells and fibroblasts. However, the ability to respond to stimulation with substance K declines rapidly in cells serum-starved for more than 24 h and in parallel with a decrease in the intracellular level of myc-gene transcripts. Contrarily, myc-transformed cells, that inspite of a decrease demonstrated a high level of myc mRNA after 48 h in serum-free medium, maintained their ability to initiate DNA synthesis when stimulated with substance K. The results suggest that the intracellular signal of substance K-induced DNA synthesis interacts with the myc protein.
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects*; D002478:Cells, Cultured; D003370:Coturnix; D003470:Culture Media; D005786:Gene Expression Regulation / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D015288:Neurokinin A; D009856:Oncogene Proteins, Viral / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D051381:Rats; D013373:Substance P / Q000031:analogs & derivatives / Q000494:pharmacology; D015320:Tachykinins; D014158:Transcription, Genetic / Q000187:drug effects; D014162:Transfection
D003470:Culture Media; D009419:Nerve Tissue Proteins; D009856:Oncogene Proteins, Viral; D011518:Proto-Oncogene Proteins; D015320:Tachykinins; D013373:Substance P; D015288:Neurokinin A; C042867:spantide