Detection of flavivirus antibodies in human serum by epitope-blocking immunoassay.
Human flavivirus group-reactive, dengue complex-reactive, and encephalitis virus complex-reactive antibodies were detected using epitope-blocking immunoassays in which the binding of selected mouse monoclonal antibodies to flavivirus antigens was blocked by human serum. When late (greater than 6 months after illness) convalescent sera were tested, the epitope-blocking immunoassays were superior to the hemagglutination inhibition test and comparable to the plaque reduction neutralization for identifying subjects immune to dengue, to Japanese encephalitis, or both viruses.
D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology*; D001667:Binding, Competitive; D003429:Cross Reactions; D003715:Dengue / Q000276:immunology; D003716:Dengue Virus / Q000276:immunology*; D004664:Encephalitis Virus, Japanese / Q000276:immunology*; D004672:Encephalitis, Japanese / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005416:Flavivirus / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D008137:Longitudinal Studies; D009500:Neutralization Tests; D011237:Predictive Value of Tests
D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes