title: Intermediate filaments in normal tissues and lymphomas of northern pike, Esox lucius L., from the Aland Islands of Finland.
abstract: A battery of polyclonal and monoclonal antibodies directed against cytokeratins, desmin, vimentin, glial fibrillary acidic protein and neurofilament proteins of mammalian species was used to demonstrate intermediate filament (IF) expression in normal and lymphoma tissues of northern pike by indirect immunofluorescence microscopy. Frozen sections of pike intestine, skin, skeletal muscle, heart, liver, testis, head-, mid-, and posterior kidney and brain demonstrated IF in a manner which strengthens the idea that they are evolutionarily highly conserved. The results also confirm the IF-subclass specificities for different types of tissues, as noted by others in other species. Pike lymphoma cells showed morphological resemblance to head kidney cells; immunofluorescence microscopy and immunoblotting revealed vimentin expression, suggesting that the Aland pike lymphoma is a true mesenchymal neoplasm and is derived from haemic cells. The significance of these studies in relation to similar work with other species and to the possible use of IFs in the classification of normal and diseased tissues in fish is discussed.
mesh_terms: D000818:Animals; D003599:Cytoskeleton / Q000473:pathology*; D003893:Desmin / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005393:Fish Diseases; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D007159:Immunologic Tests; D007381:Intermediate Filament Proteins / Q000032:analysis; D007382:Intermediate Filaments / Q000473:pathology*; D007633:Keratins / Q000032:analysis; D008223:Lymphoma / Q000473:pathology / Q000662:veterinary*; D008856:Microscopy, Fluorescence; D012487:Salmonidae; D014746:Vimentin / Q000032:analysis
chemical_list: D003893:Desmin; D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D014746:Vimentin; D007633:Keratins