Blood-group-related carbohydrate antigens are expressed on human milk galactosyltransferase and are immunogenic in rabbits.
Immunochemical evidence is presented for the presence of blood-group-related carbohydrate structures on human milk galactosyltransferase and for the occurrence of the corresponding specificities among rabbit antibodies to this enzyme. Although these carbohydrate specificities constitute minor populations among antisera and affinity-purified antibodies to galactosyltransferase, their presence is important in the immunohistochemical approach to enzyme localization, since they give rise to strong reactivities with epithelial cells of the gastrointestinal tract.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D001789:Blood Group Antigens; D002241:Carbohydrates / Q000276:immunology*; D004848:Epithelium / Q000276:immunology; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D005700:Galactosyltransferases / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007122:Immunoelectrophoresis; D008892:Milk / Q000201:enzymology*; D011817:Rabbits
D000941:Antigens; D001789:Blood Group Antigens; D002241:Carbohydrates; D000939:Epitopes; D006023:Glycoproteins; D007106:Immune Sera; D005700:Galactosyltransferases