Scanning electron microscopical observations on electrically fused human lymphocytes.
The paper describes scanning electron microscopic observations of electric field induced fusions of human lymphocytes. The cells were fused by application of short-time (20 microseconds) field pulses of 5 kV/cm after dielectrophoretic collection at about 1.2 MHz and 750 V/cm. Electron microscopy clearly demonstrates membrane fusion and the stability of initial stages of fusion.
D002459:Cell Fusion; D002462:Cell Membrane / Q000648:ultrastructure; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning