An assessment of human epidermal repair in elderly normal subjects using immunohistochemical methods.
The purpose of this study was to document a timetable for selected events of epidermal repair in standard partial thickness incised wounds on the legs of normal elderly human subjects. A Simplate-II bleeding-time device was used for producing the wounds, and immunohistochemical techniques were employed for evaluation of the wounds. Antibodies to filaggrin and Ulex europeus I demonstrated little or no staining on migrating epithelium, but staining was apparent whenever epidermal closure had occurred. Bullous pemphigoid antigen was present in the basement membrane zone at all time points examined, including beneath migrating epithelium, whereas antibodies to laminin and type IV collagen were found only at the most lateral aspects of 2-, 3-, and 5-day wounds. Staining progressed centrally by day 7 and was present as a complete linear band beneath most 14-day wounds. The Simplate-II device provides a standard, easy to use, commercially available, sterile, relatively safe method of producing wounds for systematic studies in humans.
D000368:Aged; D001324:Autoantigens / Q000032:analysis; D001760:Bleeding Time / Q000295:instrumentation; D002352:Carrier Proteins; D003094:Collagen; D003598:Cytoskeletal Proteins; D000071821:Dystonin; D004817:Epidermis / Q000032:analysis; D000091344:Filaggrin Proteins; D006801:Humans; D007633:Keratins / Q000032:analysis; D008875:Middle Aged; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D011956:Receptors, Cell Surface; D011975:Receptors, Mitogen / Q000032:analysis; D012867:Skin / Q000293:injuries* / Q000473:pathology; D014945:Wound Healing; D000097805:Collagen Type XVII
D001324:Autoantigens; D002352:Carrier Proteins; D003094:Collagen; D003598:Cytoskeletal Proteins; D000071821:Dystonin; D000091344:Filaggrin Proteins; D007633:Keratins; D009419:Nerve Tissue Proteins; D024041:Non-Fibrillar Collagens; D011956:Receptors, Cell Surface; D011975:Receptors, Mitogen; D000097805:Collagen Type XVII; C486268:DST protein, human; C000717249:FLG protein, human; C053203:Ulex europaeus lectin receptor