Generation of leukotrienes by antigen and calcium ionophore A23187 stimuli from a mouse mastocytoma cell line, MMC-16.
The generation of leukotrienes and histamine release by the mouse mastocytoma cell line MMC-16 was investigated. These cells produced leukotriene C4 (LTC4) and released histamine upon calcium ionophore A23187 and antigen stimulation. The ionophore also stimulated the biosynthesis of leukotriene B4 (LTB4) by MMC-16. Generation of LTC4 was confirmed by its characteristic UV absorption spectrum, fast atom bombardment-MS, equivalent HPLC retention time with an authentic standard and radioimmunoassay. Leukotriene B4 was characterized by its distinctive UV spectrum and HPLC retention time compared with synthetic material. IgE-mediated LTC4 generation was also observed in a dose dependent fashion with MMC-16 cells passively sensitized with monoclonal IgE specific for ovalbumin. LTC4 biosynthesis was effectively inhibited by the lipoxygenase inhibitor NDGA.
D000818:Animals; D000941:Antigens / Q000276:immunology*; D000001:Calcimycin / Q000494:pharmacology*; D006636:Histamine Release; D007073:Immunoglobulin E / Q000276:immunology; D013058:Mass Spectrometry; D012515:Mast-Cell Sarcoma / Q000378:metabolism; D051379:Mice; D011863:Radioimmunoassay; D013189:SRS-A / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured / Q000276:immunology / Q000378:metabolism*
D000941:Antigens; D013189:SRS-A; D007073:Immunoglobulin E; D000001:Calcimycin