Persistent calcium inward current in internally perfused snail neuron.
The inactivation process of the calcium current (ICa) was investigated in a molluscan neuron which was perfused intracellularly and voltage-clamped using a suction pipette technique. The decay phase of the ICa contained a very slowly inactivated component (persistent inward current; PIC). The decay time constant of this component was over 10 sec. An increase in the amplitude of the ICa or the intracellular Ca2+ concentration caused a decrease in the decay time constant of the PIC. Replacing Ba2+ with extracellular Ca2+ increased the decay time constant of the PIC. The differences in the amplitude and the decay kinetics between the ICa and the IBa resulted from changes in the amplitude and the decay time constant of the PIC. These observations support the conclusion that the inactivation of the PIC is calcium dependent [Chad, J., Eckert, R., and Ewald, D. (1984). J. Physiol. (Lond.) 347:279-300].
D000818:Animals; D001464:Barium / Q000378:metabolism / Q000494:pharmacology; D002118:Calcium / Q000037:antagonists & inhibitors / Q000502:physiology*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D003035:Cobalt / Q000494:pharmacology; D006207:Half-Life; D006372:Helix, Snails; D007424:Intracellular Fluid / Q000378:metabolism / Q000502:physiology; D007473:Ion Channels / Q000378:metabolism* / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D010477:Perfusion
D002121:Calcium Channel Blockers; D007473:Ion Channels; D001464:Barium; D003035:Cobalt; D002118:Calcium