Nuclear segmentation, ultrastructure, and cytochemistry of blood cells from dogs with Pelger-Huët anomaly.
Nuclear segmentation, ultrastructural features and cytochemical staining reactions of blood cells from 7 dogs with the heterozygous form of Pelger-Huët (P-H) anomaly were investigated. Significant nuclear hyposegmentation of granulocytes, monocytes and megakaryocytes was found, indicating a common stem cell defect in the nuclear segmentation or lobulation process. Neutrophils and eosinophils from female P-H dogs lacked sex chromatin drumsticks. Other than the suggestion of nuclear hyposegmentation and the presence of coarse heterochromatin, no ultrastructural abnormalities were discerned. Cytochemical staining by a-naphthyl acetate esterase, alkaline phosphatase, naphthol ASD chloroacetate esterase, periodic acid-Schiff, peroxidase and Sudan black B techniques gave similar results for P-H and control dog leukocytes and platelets.
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure*; D004283:Dog Diseases / Q000097:blood*; D004285:Dogs; D007962:Leukocytes / Q000166:cytology* / Q000648:ultrastructure; D008533:Megakaryocytes / Q000166:cytology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D010381:Pelger-Huet Anomaly / Q000097:blood / Q000662:veterinary*; D013194:Staining and Labeling