Inhibition of HIV-1 RNA-dependent DNA polymerase and cellular DNA polymerases alpha, beta and gamma by phosphonoformic acid and other drugs.
Potential specific inhibitors of HIV RNA-dependent DNA polymerase (RDDP) were examined in an in-vitro test system containing purified HIV-1 RDDP, or functionally purified cellular DNA-dependent DNA polymerases alpha, beta and gamma. A wide variety of drugs were tested for their specific inhibitory activity against the viral enzyme to identify substances which, at the same concentration, did not inhibit the cellular DNA polymerases. Phosphonoformic acid and derivatives were found to be the most specific inhibitors, followed by chlortetracycline.
D000998:Antiviral Agents; D004256:DNA Polymerase I / Q000037:antagonists & inhibitors; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors; D004258:DNA Polymerase III / Q000037:antagonists & inhibitors; D017245:Foscarnet; D006678:HIV / Q000187:drug effects* / Q000201:enzymology; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests; D019384:Nucleic Acid Synthesis Inhibitors; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D010746:Phosphonoacetic Acid / Q000031:analogs & derivatives / Q000494:pharmacology*; D018894:Reverse Transcriptase Inhibitors
D000998:Antiviral Agents; D019384:Nucleic Acid Synthesis Inhibitors; D009943:Organophosphorus Compounds; D018894:Reverse Transcriptase Inhibitors; D017245:Foscarnet; D004256:DNA Polymerase I; D004257:DNA Polymerase II; D004258:DNA Polymerase III; D010746:Phosphonoacetic Acid