Effect of immunoglobulin preparations on the aggregation of human erythrocytes.
The aggregation of human erythrocytes induced by four kinds of immunoglobulin preparations was examined by a low shear rheoscope. After removing anti-A+ and anti-B+ activities contaminated in all preparations by incubating with erythrocytes of different blood groups, the facilitating effect on the rouleau formation of erythrocytes was compared: (i) The effect of polyethyleneglycol-treated preparation was the same in A+-, B+-, AB+- and O+-erythrocytes. (ii) Sulfonation did not affect the velocity of rouleau formation. (iii) Some of pepsin-treated preparations showed the strongest facilitation for A+-, B+- and AB+-erythrocytes, but the facilitation was much weaker for O+-erythrocytes. The others showed the weak facilitation for all types of erythrocytes (especially O+-erythrocytes). (iv) Plasmin treatment markedly decreased the velocity of rouleau formation of AB+- and O+-erythrocytes, but was not of A+- and B+-erythrocytes.
D000328:Adult; D004338:Drug Combinations / Q000494:pharmacology; D004903:Erythrocyte Aggregation / Q000187:drug effects*; D005341:Fibrinolysin / Q000494:pharmacology; D006801:Humans; D007136:Immunoglobulins / Q000494:pharmacology*; D016756:Immunoglobulins, Intravenous; D066298:In Vitro Techniques; D008297:Male; D005719:gamma-Globulins / Q000494:pharmacology*
D004338:Drug Combinations; D007136:Immunoglobulins; D016756:Immunoglobulins, Intravenous; D005719:gamma-Globulins; C031151:pepsinated immunoglobulin G; C035483:venilon; D005341:Fibrinolysin