Effects of interferons alpha,beta,gamma alone or in combination on the phenotype of human colon carcinoma cells.
The ability of interferons (IFNs) alpha,beta,gamma, alone or in combination, to induce reversion of the phenotype of cloned human colon carcinoma cells during long-term treatment was studied. The phenotypic characteristics examined were proliferation, cellular aggregation and tumorigenicity. The combination of IFN-beta and IFN-gamma showed more antiproliferative activity than that of IFN-delta and IFN-gamma or of either IFN alone. All IFN treatments induced cellular aggregation which was associated with the appearance of brush border microvilli. Both antiproliferative effects and formation of cellular aggregates persisted only in the presence of prolonged treatment with combinations of IFNs alpha,beta with IFN-gamma. No significant differences in the binding of IFN were found between short-and long-term IFN treated cells. Treatment with IFN-beta appeared most effective in decreasing, but not completely suppressing, tumorigenicity.
D000818:Animals; D002277:Carcinoma / Q000473:pathology* / Q000628:therapy; D002449:Cell Aggregation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003110:Colonic Neoplasms / Q000473:pathology* / Q000628:therapy; D004357:Drug Synergism; D006801:Humans; D007167:Immunotherapy; D007372:Interferons / Q000008:administration & dosage / Q000494:pharmacology*; D051379:Mice; D009374:Neoplasms, Experimental / Q000628:therapy; D010641:Phenotype
D007372:Interferons