Distinct activation of Na+-H+ exchange by gastrin and CCK peptide in acini from guinea pig.
The effect of cholecystokinin (CCK)-gastrin family peptides (caerulein, unsulfated gastrin-17, and pentagastrin) and secretin in activating amiloride-sensitive 22Na uptake were investigated in guinea pig pancreatic acini. Secretin had no effect, but CCK-gastrin peptides stimulated the amiloride-sensitive 22Na uptake. The effect of caerulein was inhibited by dibutyryl guanosine 3',5'-cyclic monophosphate (cGMP) and asperlicin, indicating that activation of the Na+-H+ antiport caused by caerulein is mediated by CCK receptors. The effect of gastrin was dibutyryl cGMP and asperlicin insensitive, whereas the effect of pentagastrin was inhibited by the CCK antagonists but with a low affinity, indicating that the effect of gastrin and that of pentagastrin was CCK receptor independent. The calcium ionophore A23187 caused an increase in amiloride-sensitive 22Na uptake. However, the effect of caerulein, which increased internal calcium concentration, was not modified after depletion of intracellular calcium, and that of CCK-gastrin family peptides was not dependent on external calcium concentration. Activation of amiloride-sensitive 22Na uptake was also induced by 12-O-tetradecanoylphorbol 13-acetate and 1-oleoyl-2-acetyl-glycerol. Activation of protein kinase c may be involved in the mechanism of caerulein or gastrin in activating the Na+-H+ exchange.
D000584:Amiloride / Q000494:pharmacology; D000681:Amylases / Q000378:metabolism; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002766:Cholecystokinin / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology*; D004075:Diglycerides / Q000494:pharmacology; D005755:Gastrins / Q000494:pharmacology*; D006168:Guinea Pigs; D006859:Hydrogen / Q000378:metabolism*; D007474:Ion Exchange; D010179:Pancreas / Q000201:enzymology / Q000378:metabolism*; D010446:Peptide Fragments / Q000037:antagonists & inhibitors / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology*; D010703:Phorbol Esters / Q000494:pharmacology; D012633:Secretin / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D004075:Diglycerides; D005755:Gastrins; D010446:Peptide Fragments; D010455:Peptides; D010703:Phorbol Esters; C053399:cholecystokinin 9; D012633:Secretin; D000001:Calcimycin; D000584:Amiloride; D006859:Hydrogen; D002766:Cholecystokinin; D012964:Sodium; D000681:Amylases; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium