Expression of hepatitis B viral core region in mammalian cells.
We studied the expression of the core region of the hepatitis B virus genome in mammalian cells with recombinant plasmid vectors. Stably transformed rat fibroblast cell lines were established by transfection with vectors containing subgenomic and genome-length hepatitis B virus DNA, followed by G418 selection. The RNA transcripts directed by the core region were characterized by Northern blot hybridization and S1 nuclease mapping. Using the chloramphenicol acetyltransferase gene expression system, the promoter activity located upstream of the core open reading frame was confirmed. The synthesis of core and e polypeptides was studied with a commercial radioimmunoassay. These studies show that partial deletion of the precore sequences abolished secretion of the e antigen, but there was pronounced synthesis of the core antigen in transfected cells.
D000818:Animals; D002460:Cell Line; D004262:DNA Restriction Enzymes; D005796:Genes; D005814:Genes, Viral; D005822:Genetic Vectors; D006512:Hepatitis B Core Antigens / Q000032:analysis / Q000235:genetics*; D006513:Hepatitis B e Antigens / Q000032:analysis / Q000235:genetics; D006515:Hepatitis B virus / Q000235:genetics*; D010957:Plasmids; D011061:Poly A / Q000235:genetics; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D051381:Rats; D014158:Transcription, Genetic; D014162:Transfection
D006512:Hepatitis B Core Antigens; D006513:Hepatitis B e Antigens; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004262:DNA Restriction Enzymes