Characterization of vascular permeability-increasing component isolated from solid tumors and the effect of highly polymerized dextran sulfate on its activity.
Increase in vascular permeability is usually seen at the growth site of a tumor implant in murine dermal tissue. Increased vascular permeability was inducible by the subcutaneous injection of a solid tumor extract rich in protein precipitable at 20-50% saturation of ammonium sulfate. The vascular permeability-increasing activity of the tumor extract was reducible in the presence of highly polymerized dextran sulfate (DS-500) which showed a strong anticomplementary activity, but not by other substances such as dextran sulfate with a low molecular weight, non-sulfated dextran, chondroitin sulfate or heparin. As the tumor extract includes gamma-globulins in aggregated or bound form and adsorbs complements, it is probable that the aggregated gamma-globulins increase vascular permeability by triggering the complement activation system in the skin. DS-500 might antagonize the process.
D000645:Ammonium Sulfate / Q000494:pharmacology*; D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects; D002417:Cattle; D003169:Complement Inactivator Proteins; D016264:Dextran Sulfate; D003911:Dextrans / Q000494:pharmacology*; D006461:Hemolysis / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000032:analysis / Q000503:physiopathology*; D012867:Skin / Q000098:blood supply; D014020:Tissue Extracts / Q000494:pharmacology*
D003169:Complement Inactivator Proteins; D003911:Dextrans; D014020:Tissue Extracts; D016264:Dextran Sulfate; D000645:Ammonium Sulfate