Effect of heparin on the reactivity of SH-groups in the macrophage membrane.
The effect of heparin on macrophage (M phi) adherence and on the reactivity of membrane SH-groups to the specific SH-oxidizing agent 4,4'-dithiodipyridine (PDS) was studied. Various types of SH-reactive agents, except 5,5'-dithio-bis (2-nitrobenzoate) (DTNB), were found to inhibit adherence of mouse peritoneal M phi to serum-coated Falcon surfaces. Heparin inhibited M phi-adherence in serum containing medium and in higher concentrations stimulated the adherence inhibitory effect of PDS, especially in Ca-depleted medium. This effect of heparin may be due to its polyanionic character, as dextran sulphate but not dextran induced similar changes. The effect of heparin to increase the sensitivity of membrane SH-groups against SH-reactive agents was demonstrated also by cytotoxicity experiments. It is concluded that heparin makes the M phi-membrane unstable, by exposing some hidden SH-groups playing a role in membrane function.
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D016264:Dextran Sulfate; D003911:Dextrans / Q000494:pharmacology; D004220:Disulfides; D005260:Female; D006493:Heparin / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D066298:In Vitro Techniques; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D011725:Pyridines / Q000494:pharmacology; D013438:Sulfhydryl Compounds / Q000378:metabolism*
D003911:Dextrans; D004220:Disulfides; D011725:Pyridines; D013438:Sulfhydryl Compounds; C007542:4,4'-dipyridyl disulfide; D006493:Heparin; D016264:Dextran Sulfate; D006861:Hydrogen Peroxide; D002118:Calcium