title: Colloidal gold staining and immunoprobing of proteins on the same nitrocellulose blot.
abstract: Proteins blotted onto nitrocellulose can be stained with colloidal gold. They retain their immunoreactivity so that the stained blot can be probed with appropriate antibodies. The resulting antigen-antibody reaction is made visible by a peroxidase-coupled antispecies antibody. The blue peroxidase reaction product contrasts well with the red-stained protein pattern and allows an easy documentation by black-and-white photography.
mesh_terms: D003101:Collodion; D006046:Gold; D007120:Immunochemistry; D007124:Immunoenzyme Techniques; D011506:Proteins / Q000032:analysis* / Q000276:immunology; D013194:Staining and Labeling
chemical_list: D011506:Proteins; D006046:Gold; D003101:Collodion