Ambient ammonia effect on catalytic potential of muscle and gill AMP deaminase in fish.
AMP deaminase activity was inhibited in gill and muscle of Sarotherodon mossambicus, subjected to a week-long exposure to sublethal concentration of ambient ammonia. pH-dependent kinetic studies revealed decreased catalytic efficiency of the enzyme due to altered active site density and ionization pattern in the fish tissues at different phases of ammonia exposure.
D000659:AMP Deaminase / Q000378:metabolism*; D000641:Ammonia / Q000494:pharmacology*; D000818:Animals; D005880:Gills / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009132:Muscles / Q000201:enzymology*; D009709:Nucleotide Deaminases / Q000378:metabolism*
D000641:Ammonia; D009709:Nucleotide Deaminases; D000659:AMP Deaminase