Synthesis and retention of methotrexate polyglutamates in Ehrlich ascites carcinoma cells.
The formation of methotrexate (MTX) polyglutamates in Ehrlich ascites carcinoma parental and 5-fluorodeoxy-uridine (FdUrd)-resistant cells was examined. The prolonged retention of methotrexate in noneffluxable-polyglutamate form and the pharmacological application of this process is discussed.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002460:Cell Line; D004351:Drug Resistance; D005467:Floxuridine / Q000494:pharmacology; D006207:Half-Life; D008727:Methotrexate / Q000031:analogs & derivatives* / Q000378:metabolism; D051379:Mice; D010455:Peptides / Q000378:metabolism*; D011099:Polyglutamic Acid / Q000031:analogs & derivatives / Q000378:metabolism*
D010455:Peptides; D005467:Floxuridine; D011099:Polyglutamic Acid; C014085:methotrexate polyglutamate; D008727:Methotrexate