Synthesis and biological properties of two dimeric forms of human alpha-atrial natriuretic peptide.
Two dimeric forms of human alpha-atrial natriuretic peptide (alpha-ANP) were synthesized by solution methods and compared with monomeric alpha-ANP in terms of some biological and immunochemical properties. The parallel form (beta'-ANP) and the antiparallel form (beta-ANP) were equipotent in smooth muscle relaxant activity in isolated rat aorta and their ED50 values were estimated to be 1.7 X 10(-8) M and 1.6 X 10(-8) M, respectively. Diuretic and natriuretic responses induced by beta-ANP and beta'-ANP anesthetized rats were equally less potent but exhibited a significantly longer duration than those induced by alpha-ANP. beta-ANP and beta'-ANP possessed immunoreactivities of 60-100% and 50-90% (alpha-ANP, 100% on a molar basis), respectively, with three different antisera raised against alpha-ANP-related peptides.
D000818:Animals; D001011:Aorta; D009320:Atrial Natriuretic Factor / Q000138:chemical synthesis* / Q000276:immunology / Q000494:pharmacology; D001681:Biological Assay; D004231:Diuresis / Q000187:drug effects; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007700:Kinetics; D046911:Macromolecular Substances; D009126:Muscle Relaxation / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000502:physiology; D009318:Natriuresis / Q000187:drug effects; D010446:Peptide Fragments; D051381:Rats
D000939:Epitopes; D007106:Immune Sera; D046911:Macromolecular Substances; D010446:Peptide Fragments; D009320:Atrial Natriuretic Factor