Colony formation of granulocyte (CFU-g) and macrophage (CFU-m) precursors in serum- and albumin-free culture: effect of transferrin on clonal growth.
Clonal growth of mouse granulocyte and macrophage precursors were assayed in serum-free cultures without albumin. The number of granulocyte/macrophage colonies and clusters increased as transferrin (Trf) concentrations were increased in cultures containing serum-free L-cell-conditioned medium (LCM). On the other hand, cultures with LCM but without Trf produced relatively fewer colonies and clusters. These results indicate that Trf is one of the factors promoting the clonal growth of granulocyte and macrophage precursors in vitro. Although the presence of linoleic acid, alpha-thioglycerol, and dextran in the culture medium increased the number of granulocyte/macrophage colonies and clusters, these factors were not essential. Serum-free culture of mouse granulocyte and macrophage precursors provides a very useful system with which the activity and function of biological regulators of hematopoietic progenitors may be studied.
D000818:Animals; D001790:Blood Physiological Phenomena; D002999:Clone Cells; D003470:Culture Media; D003911:Dextrans / Q000494:pharmacology; D005990:Glycerol / Q000031:analogs & derivatives / Q000494:pharmacology; D006098:Granulocytes / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects*; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012709:Serum Albumin / Q000494:pharmacology; D014168:Transferrin / Q000494:pharmacology*
D003470:Culture Media; D003911:Dextrans; D008041:Linoleic Acids; D012709:Serum Albumin; D014168:Transferrin; D019787:Linoleic Acid; C009465:thioglycerol; D005990:Glycerol