Localization of proteoglycan within the extracellular matrix sheath of cone photoreceptors.
The interphotoreceptor matrix of the human retina was examined histochemically by staining with a cationic copper phthalocyanin dye, Cuprolinic Blue, in a critical electrolyte concentration method which allowed staining of sulfated polyanions. In the presence of Cuprolinic Blue, a dense punctate matrix component was visualized covering the cone from the outer limiting membrane of the retina to the tip of the cone outer segment and extending through the matrix to the apical surface of the pigment epithelium. The size and appearance of the stained material are consistent with its being proteoglycan and its distribution is consistent with its being a component of the cone extracellular matrix sheath. A fine reticular pattern was observed in the presence and absence of Cuprolinic Blue.
D005109:Extracellular Matrix / Q000032:analysis; D006651:Histocytochemistry; D006801:Humans; D007211:Indoles; D008274:Magnesium; D015636:Magnesium Chloride; D008854:Microscopy, Electron; D009942:Organometallic Compounds; D010786:Photoreceptor Cells / Q000032:analysis* / Q000648:ultrastructure; D011509:Proteoglycans / Q000032:analysis*; D013194:Staining and Labeling
D007211:Indoles; D009942:Organometallic Compounds; D011509:Proteoglycans; D015636:Magnesium Chloride; C015445:copper phthalocyanine; D008274:Magnesium