A sensitive urea-silver stain method for detecting trace quantities of separated proteins in polyacrylamide gels.
An ultrasensitive method using a urea-silver staining procedure to detect trace quantities of proteins in polyacrylamide gels (PAGE) is described. This technique is sensitive enough to detect picogram quantities of proteins resolved on sodium dodecyl sulfate-polyacrylamide gels. The major advantages of our method are that it provides a clear background and it is more sensitive than other techniques allowing it to substitute for radioisotopic techniques in some cases.
D004591:Electrophoresis, Polyacrylamide Gel; D011506:Proteins / Q000302:isolation & purification*; D012834:Silver; D013194:Staining and Labeling / Q000379:methods*; D014508:Urea
D011506:Proteins; D012834:Silver; D014508:Urea