Effects of harmaline on ion transport and oxygen consumption by the bovine tracheal epithelium.
The alkaloid harmaline is known to affect various membrane transport systems. This study examines the action of the drug on the short-circuit current (I0) and on the oxidative metabolism (Jr) in the tracheal epithelium of the cow. In this tissue I0 corresponds to the sum of two active transports: Na+ is absorbed and Cl- is secreted by a process based on the activity of the Na+ pump. A well defined relationship has been previously demonstrated between these active transports and the rate of O2 consumption (Schoenenweid et al., 1984 b). Low concentrations of harmaline (10(-6) to 5.10(-6) M) induced a small stimulation of I0. In contrast, larger concentrations (between 5.10(-5) and 10(-3) M) yielded a dose-related inhibition of I0, with an apparent concentration yielding 50% of maximal effect of 7.1.10(-4) M and maximal effect approaching 100%. The action was fully reversible after removal of the drug. The measurements of the fluxes of 22Na and 36Cl revealed that harmaline at a concentration of 8.10(-4) M, which decreased the I0 by 74 +/- 1% (n = 23), diminished both Na+ and Cl- transports, by 81 and 52%, respectively. The time course of I0 decay following the administration of harmaline was made of three components, with half-times of 0.34, 2.2 and 15.2 min. The time course was not appreciably modified when Cl- secretion was abolished with furosemide. Although harmaline, 10(-3)M, inhibited markedly I0, it did not modify Jr significantly. In contrast, when K+ in the incubation solution was omitted, both Ji and Jr were lowered.(ABSTRACT TRUNCATED AT 250 WORDS)
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002713:Chlorine / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D005260:Female; D006246:Harmaline / Q000494:pharmacology*; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects*; D010101:Oxygen Consumption / Q000187:drug effects*; D012964:Sodium / Q000378:metabolism*; D013997:Time Factors; D014132:Trachea / Q000378:metabolism*
D000470:Alkaloids; D007473:Ion Channels; D002713:Chlorine; D012964:Sodium; D006246:Harmaline