Inflammation-induced synthesis of proteoheparan sulfate: a novel acute-phase reactant in rat hepatocytes.
The synthesis of proteoheparan sulfate in hepatocytes is positively regulated under acute-phase conditions produced either by turpentine or deep back incision. In both cases the incorporation of [35S]sulfate and [14C]glucosamine is doubled during a 4-h incubation period if compared with control rat hepatocytes. Neither the fractional secretion rate of heparan sulfate into the medium (less than 0.1 of cell-associated glycosaminoglycans) nor the composition of newly formed proteoglycans in hepatocytes are affected during acute phase reaction.
D000209:Acute-Phase Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D000818:Animals; D002250:Carbon Radioisotopes; D011508:Chondroitin Sulfate Proteoglycans / Q000096:biosynthesis* / Q000302:isolation & purification; D005944:Glucosamine / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate / Q000096:biosynthesis* / Q000302:isolation & purification; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D011509:Proteoglycans / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D013462:Sulfur Radioisotopes; D014425:Turpentine / Q000009:adverse effects
D000209:Acute-Phase Proteins; D002250:Carbon Radioisotopes; D011508:Chondroitin Sulfate Proteoglycans; D005944:Glucosamine; D006025:Glycosaminoglycans; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D011509:Proteoglycans; D013462:Sulfur Radioisotopes; D014425:Turpentine