title: Effects of fibrinogen, globulin, albumin and hematocrit on the kinetics of erythrocyte aggregation in man.
abstract: The effects of fibrinogen, globulin, albumin, and packed blood cell volume (hematocrit) on the kinetics of erythrocyte aggregation (RBC-A) after stasis were quantitatively evaluated by recording the changes of light transmission through the blood in transparent tubing with an apparatus consisting of a light-emitting diode and a silicon photodiode. The measurements were done on samples of heparinized whole blood, suspensions of erythrocytes containing various concentrations of fibrinogen (0.5 to 25 g/L) or gamma globulin (25 to 150 g/L), and blood of various hematocrit values prepared from three samples of whole blood having different concentrations of fibrinogen. The half-time of the increase of light transmission after the flow-stop was used as an index of the kinetics of RBC-A (T1/2). This simple and rapid method for estimating erythrocyte aggregation had excellent reproducibility (N = 51, r = 0.99). The values of T1/2 showed significant negative correlations with the concentrations of fibrinogen and globulin but a positive correlation with that of albumin. The T1/2 value decreased markedly and then remained almost constant with increase in the value of hematocrit, but the influence of hematocrit on T1/2 was markedly dependent on the concentration of fibrinogen.
mesh_terms: D001798:Blood Proteins / Q000032:analysis / Q000494:pharmacology*; D004903:Erythrocyte Aggregation; D004906:Erythrocyte Count; D005340:Fibrinogen / Q000494:pharmacology; D006400:Hematocrit; D006801:Humans; D007700:Kinetics; D012709:Serum Albumin / Q000494:pharmacology; D014172:Transillumination / Q000379:methods; D005719:gamma-Globulins / Q000494:pharmacology
chemical_list: D001798:Blood Proteins; D012709:Serum Albumin; D005719:gamma-Globulins; D005340:Fibrinogen