Evidence for a selective localization of voltage-sensitive Ca2+ channels in nerve cell bodies of corpus striatum.
Specific binding sites for [3H]nitrendipine, an organic Ca2+ channel antagonist, were abolished in crude synaptosomal membranes of kainic acid-lesioned caudate nuclei. In contrast, specific lesions of dopaminergic or serotonergic axon terminals in caudate nuclei failed to alter the density or the affinity of [3H]nitrendipine binding sites. In addition, the basal and veratridine-stimulated 45Ca2+ accumulations were greatly impaired in slices prepared from kainic acid-lesioned caudate nuclei. The veratridine-elicited accumulation of 45Ca2+ in control slices was attenuated by addition of tetrodotoxin in the incubation medium. The present data provide evidence that most of the [3H]nitrendipine binding sites and the voltage-dependent Ca2+ channels are located in intrinsic neurons or interneurons in caudate nucleus. In contrast, destruction of dopaminergic or serotonergic nerve terminals emanating from other brain areas and innervating the caudate nucleus failed to change the apparent Bmax value for [3H]nitrendipine binding.
D015116:5,7-Dihydroxytryptamine / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism*; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D003342:Corpus Striatum / Q000166:cytology* / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006892:Hydroxydopamines / Q000008:administration & dosage / Q000494:pharmacology; D007276:Injections, Intraventricular; D007473:Ion Channels / Q000378:metabolism*; D007608:Kainic Acid / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D009474:Neurons / Q000378:metabolism*; D009568:Nitrendipine / Q000378:metabolism; D016627:Oxidopamine; D051381:Rats; D011919:Rats, Inbred Strains; D014701:Veratridine / Q000494:pharmacology
D006892:Hydroxydopamines; D007473:Ion Channels; D015116:5,7-Dihydroxytryptamine; D014701:Veratridine; D016627:Oxidopamine; D009568:Nitrendipine; D002795:Choline O-Acetyltransferase; D007608:Kainic Acid; D002118:Calcium