Deplasticizing of thick Epon sections involving a new adhesive technique and staining of nervous tissue.
The present communication deals with a technique developed for the selective staining of neural tissue in thick (10 micron) Epon sections. A new adhesive method was needed, because the known techniques are only applicable to 0.5-2 micron thin sections. The critical step in the procedure is the adhesion of the sections onto the slides. This is accomplished by heating the sections on top of a uniform layer of albumin glycerol on the slide followed by coating with celloidin. The results after deplasticizing and coagulation with this technique are comparable to those obtained by paraffin or frozen section techniques, but in addition have the advantage of Epoxy resin embedding e.g. the possibility of cutting undecalcified hard tissues and sections for serial reconstruction.
D000269:Adhesives; D000418:Albumins; D000818:Animals; D001502:Beak / Q000033:anatomy & histology / Q000294:innervation; D001717:Birds; D003101:Collodion; D004853:Epoxy Resins; D005990:Glycerol; D008465:Mechanoreceptors / Q000033:anatomy & histology; D009417:Nerve Tissue / Q000033:anatomy & histology*; D013194:Staining and Labeling / Q000379:methods*
D000269:Adhesives; D000418:Albumins; D004853:Epoxy Resins; C004875:EPON; D003101:Collodion; D005990:Glycerol