Adsorption of proteins by chrysotile and crocidolite: role of molecular weight and charge density.
Transferrin, gamma-globulin, fibrinogen, aldolase, albumin, and cytochrome c at concentration of 0.45 muMole/liter were treated with chrysotile and crocidolite fibers. Specific adsorption for each protein and correlations between protein molecular weight and charge density in experimental conditions were evaluated. Chrysotile showed the highest absorption capability, particularly toward albumin and cytochrome c. Affinity for fibers was poorly linked to protein molecular weight; on the contrary, a good correlation with protein charge density was found. The sign of charge on fibers and proteins seemed to play a minor role in adsorption.
D000327:Adsorption; D000418:Albumins; D000818:Animals; D001194:Asbestos; D017638:Asbestos, Crocidolite; D017632:Asbestos, Serpentine; D003574:Cytochrome c Group; D004563:Electrochemistry; D005340:Fibrinogen; D005634:Fructose-Bisphosphate Aldolase; D006801:Humans; D008970:Molecular Weight; D011506:Proteins; D014168:Transferrin; D005719:gamma-Globulins
D000418:Albumins; D017632:Asbestos, Serpentine; D003574:Cytochrome c Group; D011506:Proteins; D014168:Transferrin; D005719:gamma-Globulins; D017638:Asbestos, Crocidolite; D001194:Asbestos; D005340:Fibrinogen; D005634:Fructose-Bisphosphate Aldolase