Canine limbic system substance P receptors.
A canine limbic system preparation is utilized as a source of substance P receptor(s) to screen gradient RP-HPLC fractions for the presence of receptoractive-substance P activity, and to quantify endogenous receptoractive-substance p in biological extracts such as human tooth pulp. The binding characteristics, KD = 1.3 nM and Bmax = 11 fmol mg-1 protein, are similar to values obtained from receptors produced from other biological sources such as whole rat brain, minus cerebellum.
D000818:Animals; D003782:Dental Pulp / Q000378:metabolism; D004285:Dogs; D004744:Enkephalin, Methionine / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D008032:Limbic System / Q000187:drug effects / Q000378:metabolism*; D011869:Radioligand Assay; D018040:Receptors, Neurokinin-1; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000378:metabolism*; D013373:Substance P / Q000378:metabolism / Q000494:pharmacology
D018040:Receptors, Neurokinin-1; D017981:Receptors, Neurotransmitter; D013373:Substance P; D004744:Enkephalin, Methionine