Lack of synthesis of pentamer IgM in Xenopus oocytes after injection of poly(A)+ RNA from hybridoma cells.
Total poly(A)+ RNA was isolated from B cell hybridomas producing monoclonal antibodies of the IgM class specific for sheep red blood cells. These RNAs were microinjected into Xenopus oocytes and the translation products were analyzed. Unlike the hybridoma cells, the oocytes synthetized only the light chains and oligomer IgM, but evidence for assembly of functional pentamer IgM was not obtained. The possible reasons for these differences are discussed.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D005260:Female; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000235:genetics*; D009865:Oocytes / Q000378:metabolism*; D011061:Poly A / Q000235:genetics*; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012756:Sheep; D014981:Xenopus
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA