Effect of long-term storage on monoamine metabolite levels in human cerebrospinal fluid.
Concentrations of homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA) were measured in human cerebrospinal fluid (CSF) following long-term storage at -20 degrees C for intervals of three to 60 months. No significant changes in HVA levels were detected in CSF stored for up to 60 months. On the other hand, 5-HIAA concentrations remained stable for up to 6 months, but decreased significantly in the specimens stored for longer time intervals. The results indicate that 5-HIAA should be determined within 6 months after CSF collection, while HVA determinations may be delayed.
D002851:Chromatography, High Pressure Liquid; D005188:False Negative Reactions; D005615:Freezing; D006719:Homovanillic Acid / Q000134:cerebrospinal fluid*; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000134:cerebrospinal fluid*; D011309:Preservation, Biological; D013997:Time Factors
D006897:Hydroxyindoleacetic Acid; D006719:Homovanillic Acid