Determination of estrogen receptors in human breast cancer: comparison between enzyme immunoassay and dextran-coated charcoal method.
Estrogen receptor determination was performed on 120 breast cancer cytosols, using the dextran-coated charcoal method (DCC) and an enzyme immunoassay (EIA) to compare the efficiency of the two techniques. A strong correlation was noted between ER concentrations determined by DCC and EIA (P less than 0.001). The mean ER-EIA value was significantly higher than the mean ER-DCC value in premenopausal (P less than 0.001) as well in postmenopausal (P less than 0.001) patients.
D001943:Breast Neoplasms / Q000032:analysis*; D002606:Charcoal; D003911:Dextrans; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008722:Methods; D011960:Receptors, Estrogen / Q000032:analysis*
D003911:Dextrans; D011960:Receptors, Estrogen; D002606:Charcoal