title: Isolated canine mastocytoma cells: propagation and characterization of two cell lines.
abstract: Five different dog mastocytoma tumors were successfully transplanted and propagated in BALB/c nude mice. Cells from two of these tumors were passaged serially through at least four generations of mice without morphological or functional change. The average yield from a 2-cm tumor harvested from a mouse was 1.2 +/- 2.8 X 10(9) mast cells with greater than 90% viability. Cells of one line were larger and more heavily granulated than the other, and contained 1.29 +/- 0.74 pg histamine/cell (mean +/- SD). Calcium ionophore A23187 and compound 48/80 caused dose dependent histamine release with no significant difference in release from generation to generation. The smaller cells contained 0.06 +/- 0.06 pg histamine/cell. Histamine release after calcium ionophore or compound 48/80 was dose dependent and unchanged through serial passages. Following passive sensitization antigen caused dose-dependent histamine release confirming the presence of IgE receptors on these cells. In both cell lines histamine release was inhibited by terbutaline, dibutyryl adenosine 3',5'-cyclic monophosphate, or isobutylmethylxanthine. These methods provide a morphologically and functionally stable population of nearly pure canine mast cells for biochemical and physiological studies.
mesh_terms: D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002460:Cell Line; D046508:Culture Techniques / Q000379:methods; D004285:Dogs; D006632:Histamine / Q000032:analysis; D006636:Histamine Release / Q000187:drug effects; D012515:Mast-Cell Sarcoma / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D051379:Mice; D008819:Mice, Nude; D008854:Microscopy, Electron; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
chemical_list: D000001:Calcimycin; D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine