Effect of protein kinase C activation on mast cell histamine release.
The role of protein Kinase C activators in the process of histamine secretion has been studied in rat peritoneal mast cells purified by a density gradient. TPA (12-O-tetradecanoyl-phorbol-13-acetate), a tumor promoter which activates protein kinase C, induced histamine release in the presence and in the absence of external free Ca2+. TPA and the calcium ionophore A23187 have an additive effect on secretion. Histamine release induced by TPA is energy-dependent. In the presence of 100 microM KCN secretion was moderately inhibited, however when glucose was removed from the incubation medium TPA-induced histamine release in the presence of KCN was strongly depressed.
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002470:Cell Survival; D004789:Enzyme Activation; D005947:Glucose / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008407:Mast Cells / Q000166:cytology / Q000201:enzymology / Q000502:physiology*; D011190:Potassium Cyanide / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000001:Calcimycin; D011493:Protein Kinase C; D005947:Glucose; D011190:Potassium Cyanide; D013755:Tetradecanoylphorbol Acetate