title: One- and two-dimensional 90.5-MHz 13C-NMR spectroscopy of the N-linked triantennary oligosaccharide units of calf fetuin.
abstract: Complete assignments of all anomeric resonances in the proton and carbon spectra of the N-linked oligosaccharide units of fetuin were made using one- and two-dimensional NMR spectroscopy. We are able to confirm the presence of microheterogeneity in the N-acetylneuraminic acid linkages to the galactose residues and the presence of a unique triantennary structure which carries a side chain: NeuAc alpha(1-3)Gal beta(1-3)GlcNac beta(1-4)-. Anomeric carbon chemical shifts changes resulting from long-range conformational effects were observed.
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002417:Cattle; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D019158:N-Acetylneuraminic Acid; D009844:Oligosaccharides; D011522:Protons; D012794:Sialic Acids; D000509:alpha-Fetoproteins
chemical_list: D009844:Oligosaccharides; D011522:Protons; D012794:Sialic Acids; D000509:alpha-Fetoproteins; D019158:N-Acetylneuraminic Acid