Modification of fibrin network ultrastructure by Fab fragments specific for different domain of fibrinogen.
Kinetics of inhibition of fibrin monomer polymerization produced by Fab fragments prepared from immunochemically purified monospecific antibodies to the surface epitopes of different domains of fibrinogen molecule has been correlated with electron microscopic observations of resulting specimens. Fab fragments prepared from anti FgD antisera were the most efficient inhibitors of thrombin-catalysed conversion of fibrinogen to fibrin; polymerization of fibrin monomers as detected spectrophotometrically was abolished at 2:1 molar ratio of anti FgD Fab fragments to fibra monomer. These Fab fragments acting as a steric hindrance of polymerization sites inhibited the first stage of fibrin monomer aggregation. Interaction of Fab fragments derived from antibodies specific for alpha 239-476 with corresponding segment of fibrinogen molecule resulted in a weak inhibition of fibrin monomer polymerization. However, fibrin obtained in the presence of these Fab fragments was significantly modified and showed no periodicity. This observation may suggest that anti alpha 239-476 Fab impaired the course of the second stage of fibrin monomer polymerization, i.e. lateral association of fibrin fibrils.
D000939:Epitopes / Q000276:immunology; D005337:Fibrin / Q000276:immunology / Q000378:metabolism*; D005340:Fibrinogen / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D007700:Kinetics; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D013053:Spectrophotometry; D013917:Thrombin / Q000378:metabolism
D000939:Epitopes; D007106:Immune Sera; D007140:Immunoglobulin Fab Fragments; D046911:Macromolecular Substances; D005337:Fibrin; D005340:Fibrinogen; D013917:Thrombin