Cloning of genomic fragment from Plasmodium chabaudi expressing a 105 kilodalton antigen epitope.
The 105 kDa antigen of Plasmodium chabaudi has many characteristics of the P. falciparum Pf 155 (RESA) molecule. A clone (pPC105e) from a P. chabaudi genomic library was isolated using immune screening with a 105 kDa antigen specific monoclonal antibody (B7E10). Southern, Northern and Western blotting analyses provide evidence for a lack of variability at the protein and DNA levels. A subclone of the insert in the expression vector pEX2, synthesises a fusion peptide which contains the epitope recognized by B7E10. Sequences homologous to the insert were detected in the genome of three other rodent and two primate malarias.
D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000235:genetics; D007118:Immunoassay; D009693:Nucleic Acid Hybridization; D010961:Plasmodium / Q000235:genetics* / Q000276:immunology; D012313:RNA / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000032:analysis
D000953:Antigens, Protozoan; D000939:Epitopes; D011993:Recombinant Fusion Proteins; D012313:RNA; D004247:DNA; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI