Regulation of alpha- and beta-tubulin mRNAs in rat brain during synaptogenesis.
Developmental alterations in alpha- and beta-tubulin mRNA in polysomes from brains of -3 days (fetal) to 30-day-old rats were quantitated by using well-characterized chicken alpha- and beta-tubulin cDNA probes, pT1 and pT2 having 80-90% homology with rat sequences. Northern blot analysis revealed a single major (greater than 95%) 1.8 kb mRNA for both alpha- and beta-tubulin. Quantitation by slot hybridization indicates a virtually coordinate expression of alpha- and beta-tubulin mRNA with a maximal level around day 5 after birth, which represents the mid-phase of synaptogenesis.
D000818:Animals; D001921:Brain / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D004274:DNA, Recombinant; D005333:Fetus; D005786:Gene Expression Regulation; D009693:Nucleic Acid Hybridization; D011061:Poly A; D011132:Polyribosomes / Q000378:metabolism; D012313:RNA; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011988:Receptors, Thyroid Hormone / Q000378:metabolism; D013569:Synapses / Q000502:physiology*; D014404:Tubulin / Q000378:metabolism*
D004274:DNA, Recombinant; D012333:RNA, Messenger; D011988:Receptors, Thyroid Hormone; D014404:Tubulin; D011061:Poly A; D012313:RNA