Development and characterization of trinitrophenyl-specific L3T4+ T-cell clones.
Two antigen-specific, L3T4+, Lyt-2- T-cell clones have been developed from (C57BL/6 x C3H/HeN) F1 mice epicutaneously sensitized to trinitrochlorobenzene. Genetic mapping and antibody blocking studies demonstrated that both of these clones have specificity for trinitrophenyl in association with Ek beta:Ek alpha or Eb beta:Ek alpha Ia molecules. One of the clones (D-8) also recognizes nonhaptenated cells expressing Es beta:Ek alpha. These clones should provide useful tools for the assessment of the signals required for triggering immune responses to haptenated self antigens.
D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000235:genetics; D002455:Cell Division; D002999:Clone Cells / Q000276:immunology; D000939:Epitopes / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006241:Haptens / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D010641:Phenotype; D010853:Picryl Chloride / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000939:Epitopes; D006241:Haptens; D010853:Picryl Chloride