[Use of immunological adsorption in heterogeneous immunoenzyme analysis in determining antibodies to the protective determinants of Bacillus anthracis].
In a heterogeneous enzyme immunoassay system involving the use of polystyrene assay plates, the method of immunological adsorption has been used for studying the spectrum of specific antibodies to individual chromatographically pure fractions of B. anthracis toxin. The relationship between the characteristics of acquired stability and the level of serum antibodies to individual biologically active and biologically inactive toxin antigens in guinea pigs, immunized with live vaccines in a single injection, has been studied. As revealed in this study, the level of serum antibodies to chromatographically pure toxin fractions does not reflect acquired immunity to anthrax.
D000327:Adsorption; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology* / Q000302:isolation & purification; D001408:Bacillus anthracis / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology* / Q000302:isolation & purification; D006168:Guinea Pigs; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D013997:Time Factors
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D000939:Epitopes
Primenenie immunologicheskoĭ adsorbtsii v geterogennom immunofermentnom analize pri opredelenii antitel k protektivnym determinantam Bacillus anthracis.