Lectin-labeled membrane is transferred to the Golgi complex in mouse pituitary cells in vivo.
Labeling of the Golgi complex with the lectin conjugate wheat germ agglutinin-horseradish peroxidase (WGA-HRP), which binds to cell surface membrane and enters cells by adsorptive endocytosis, was analyzed in secretory cells of the anterior, intermediate, and posterior lobes of mouse pituitary gland in vivo. WGA-HRP was administered intravenously or by ventriculo-cisternal perfusion to control and salt-stressed mice; post-injection survival times were 30 min-24 hr. Peroxidase reaction product was identified within the extracellular clefts of anterior and posterior pituitary lobes through 24 hr but was absent in intermediate lobe. Endocytic vesicles, spherical endosomes, tubules, dense and multivesicular bodies, the trans-most saccule of the Golgi complex, and dense-core secretory granules attached or unattached to the trans Golgi saccule were peroxidase-positive in the different types of anterior pituitary cells and in perikarya of supraoptico-neurohypophyseal neurons; endoplasmic reticulum and the cis and intermediate Golgi saccules in the same cell types were consistently devoid of peroxidase reaction product. Dense-core secretory granules derived from cis and intermediate Golgi saccules in salt-stressed supraoptic perikarya likewise failed to exhibit peroxidase reaction product. The results suggest that in secretory cells of anterior and posterior pituitary lobes, WGA-HRP, initially internalized with cell surface membrane, is eventually conveyed to the trans-most Golgi saccule, in which the lectin conjugate and associated membrane are packaged in dense-core secretory granules for export and potential exocytosis of the tracer. Endoplasmic reticulum and the cis and intermediate Golgi saccules appear not to be involved in the endocytic/exocytic pathways of pituitary cells exposed to WGA-HRP.
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D004705:Endocytosis; D005260:Female; D006056:Golgi Apparatus / Q000378:metabolism*; D006735:Horseradish Peroxidase; D037102:Lectins; D051379:Mice; D008853:Microscopy; D008854:Microscopy, Electron; D009940:Organoids / Q000378:metabolism*; D010902:Pituitary Gland / Q000378:metabolism*; D037121:Plant Lectins; D012965:Sodium Chloride; D030262:Soybean Proteins; D013312:Stress, Physiological / Q000378:metabolism; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D014909:Wheat Germ Agglutinins; D010835:Phytohemagglutinins
D006735:Horseradish Peroxidase; D037102:Lectins; D037121:Plant Lectins; D012965:Sodium Chloride; D030262:Soybean Proteins; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D014909:Wheat Germ Agglutinins; C028824:soybean lectin; D010835:Phytohemagglutinins