[Homology between antigenic determinants of bovine clathrin and clathrin from the brown alga Laminaria digitata].
Coated vesicles, essential organelles of intracellular membrane traffic, have been extensively studied in animal and higher plant cells. In the algae, cytological studies only have been performed which demonstrate the presence of such coated vesicles with their surrounding clathrin lattice. The present work has been carried out on coated vesicles isolated for the first time from the brown algae Laminaria digitata. For comparison of the antigenic characteristics of clathrin prepared from the Bovine brain or adrenocortical cells and the clathrin prepared from algae, polyclonal antibodies have been raised to a purified Bovine brain clathrin in Goat and to Bovine adrenocortical clathrin in Rabbit. The positive immunological responses of the coated vesicles and the clathrin from Algae to these antibodies, evidence an homology between antigenic determinants of clathrin from animal and vegetal cells.
D000302:Adrenal Cortex / Q000032:analysis; D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002966:Clathrin / Q000276:immunology* / Q000302:isolation & purification; D003034:Coated Pits, Cell-Membrane / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology*; D056890:Eukaryota / Q000276:immunology*; D000459:Phaeophyceae / Q000276:immunology*
D002966:Clathrin; D000939:Epitopes
Homologie entre des déterminants antigéniques de clathrine de boeuf et de clathrine d'une algue brune Laminaria digitata.