Simultaneous detection of B-cells and T-cells by a double immunohistochemical technique using immunogold-silver staining and the avidin-biotin-peroxidase complex method.
A new double immunohistochemical technique for the simultaneous detection of B-cells and T-cells was investigated, using tissue preparations obtained from human axillary lymph nodes and rejected renal allografts. The specimens were immunostained first for the demonstration of B-cells, by the immunogold-silver staining (IGSS) method using Leu-12 monoclonal antibody, and then for T-cells by the avidin-biotin-peroxidase complex (ABC) method using Leu-1 monoclonal antibody. With the present methods, both B-cells and T-cells were clearly detected and distinctively identified without cross-linking of antibodies or double reaction of enzymes.
D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis; D001402:B-Lymphocytes / Q000166:cytology*; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D007124:Immunoenzyme Techniques; D008198:Lymph Nodes / Q000166:cytology; D013194:Staining and Labeling; D013601:T-Lymphocytes / Q000166:cytology*
D000911:Antibodies, Monoclonal; D000941:Antigens