Thiazole orange: a new dye for reticulocyte analysis.
The purpose of this study was to find a 488-nm excitable fluorescent dye for reticulocyte analysis by the use of fluorescence activated cell cytometry. The chemical structure of thioflavin T, a dye used for reticulocyte analysis with 457-nm excitation, was used as a model. Several dyes were synthesized and evaluated by quantum yield determination, fluorescence microscopy, and flow cytometry. The best results were obtained with a dye we have named "thiazole orange"; analysis of several blood samples with thiazole orange gave a correlation coefficient of 0.97 as compared to a manual determination of reticulocyte percentage.
D052160:Benzothiazoles; D002452:Cell Count; D004247:DNA / Q000378:metabolism; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006801:Humans; D011804:Quinolines; D012313:RNA / Q000378:metabolism; D012156:Reticulocytes / Q000166:cytology / Q000378:metabolism*; D013844:Thiazoles
D052160:Benzothiazoles; D005456:Fluorescent Dyes; D011804:Quinolines; D013844:Thiazoles; C051000:thiazole orange; C009462:thioflavin T; D012313:RNA; D004247:DNA