GTP gamma S-induced solubilization of actin and myosin from rabbit peritoneal neutrophil membrane.
Addition of guanosine 5'-(3-O-thio)triphosphate (GTP gamma S) to the membrane fraction isolated from rabbit peritoneal neutrophils results in the solubilization of several proteins from the membrane. The major proteins are of 180 kDa (myosin) and 43 kDa (actin). The effect is observed with a half-maximum GTP gamma S concentration of 70 microM. The potencies of various nucleotides are compared: GTP gamma S greater than GTP greater than ATP greater than GDP, GMP, cGMP, cAMP. The effect does not require calcium and is not inhibited by using membranes prepared from cells that have been pretreated with pertussis toxin.
D000199:Actins / Q000302:isolation & purification*; D000818:Animals; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate / Q000031:analogs & derivatives* / Q000494:pharmacology; D008565:Membrane Proteins / Q000302:isolation & purification*; D009218:Myosins / Q000302:isolation & purification*; D009504:Neutrophils / Q000032:analysis*; D010529:Peritoneal Cavity; D011817:Rabbits; D012394:Rosaniline Dyes; D012995:Solubility; D013194:Staining and Labeling; D013873:Thionucleotides / Q000494:pharmacology*
D000199:Actins; D008565:Membrane Proteins; D012394:Rosaniline Dyes; D013873:Thionucleotides; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate; D009218:Myosins; C004692:coomassie Brilliant Blue