Clonal diversity of myelin basic protein-specific T lymphocytes.
A panel of myelin basic protein (MBP)-specific, class II major histocompatibility complex (As)-restricted T-cell clones were established from SJL/J mice. Three clonotypes, based on their responses to guinea pig MBP and its peptide fragments, were observed. Clonotype I cells, represented by clones HS.6, HS.D2, HS.8, HS.E10, and HS.C1, were reactive to the encephalitogenic C-terminal fragment of MBP, amino acid residues 89-169. Clonotype II, represented by clone HS.E3, was reactive to fragments containing residues 43-88, and clones HS.D12 and HS.C7, representing clonotype III cells, responded to the whole molecule only. Three clones from clonotype I were capable of transferring both clinical and histological signs of experimental allergic encephalomyelitis (EAE) into naive mice. Southern blot analysis of T-cell receptor beta-chain genes using J beta 1- and J beta 2-specific probes showed that the rearrangement pattern was unique in each of the clones. These results suggest that the development of EAE may represent an autoaggressive polyclonal T-cell response.
D000818:Animals; D002999:Clone Cells / Q000276:immunology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology; D005260:Female; D006168:Guinea Pigs; D051379:Mice; D008815:Mice, Inbred Strains / Q000276:immunology; D004676:Myelin Basic Protein / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000637:transplantation
D004676:Myelin Basic Protein; D010446:Peptide Fragments