Feedback regulation of the rplJL-rpoBC ribosomal protein operon of Escherichia coli requires a region of mRNA secondary structure.
The rplJ-rpoBC (L10) operon of Escherichia coli is regulated in part through translational repression (feedback regulation) by ribosomal protein L10 or a complex of ribosomal proteins L10 and L7/L12 (L10-L7/L12). We have constructed mutants in the untranslated leader region of a rplJ-lacZ fusion by oligonucleotide-directed mutagenesis. The mutations include several deletions and a number of single base changes, all of which fail to exhibit normal feedback regulation. Chemical probing of part of the rplJ mRNA leader in the mutagenized region confirms that all of the mutations lie in a stem structure located 140 nucleotides upstream from the translation start-site. The structure includes a 12 base-pair stem, a four base stem-loop, and a six base bulge-loop. Point mutations that abolish feedback regulation are presumed to disrupt this stem structure. Pseudorevertants of selected point mutations were constructed by combining pairs of single base mutations. In these cases, both the secondary structure of the RNA and feedback regulation were restored. The results allow us to define a region of secondary structure in the rplJ mRNA leader that is necessary for feedback regulation.
D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D005246:Feedback; D005786:Gene Expression Regulation; D009154:Mutation; D009690:Nucleic Acid Conformation; D009876:Operon; D010957:Plasmids; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012269:Ribosomal Proteins / Q000235:genetics*
D012329:RNA, Bacterial; D012333:RNA, Messenger; D012269:Ribosomal Proteins