Effects of ethanol, acetaldehyde and disulfiram on the metabolism of biogenic aldehydes in isolated human blood cells and platelets.
The effects of ethanol, acetaldehyde and disulfiram on the metabolism of biogenic aldehydes were measured in different human blood fractions. Intact erythrocytes, leukocytes and platelets were incubated in phosphate-buffered saline with 3,4-dihydroxyphenylacetaldehyde (DOPAL) or 5-hydroxy-indole-3-acetaldehyde (5-HIAL), the aldehydes derived from dopamine and serotonin, respectively. The disappearance of the aldehyde and the formation of acid and alcohol metabolites were analysed in the presence of different concentrations of ethanol, acetaldehyde or disulfiram using high-performance liquid chromatography with electrochemical detection. Ethanol at a concentration of 20 mM did not affect the biogenic aldehyde metabolism. High concentrations of acetaldehyde caused a dose-dependent inhibition of the disappearance rate of the biogenic aldehydes and of the formation rate of acid metabolites. In incubations with leukocytes or platelets, the inhibition of the acid formation was associated with a slight increase in the formation of the alcohol metabolites. Disulfiram at a concentration of 50 microM totally inhibited the metabolism of DOPAL and 5-HIAL in incubations with erythrocytes or platelets, whereas much less inhibition was observed in incubations with leukocytes.
D015102:3,4-Dihydroxyphenylacetic Acid / Q000031:analogs & derivatives / Q000097:blood*; D000079:Acetaldehyde / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D004221:Disulfiram / Q000494:pharmacology*; D004298:Dopamine / Q000097:blood; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology*; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000031:analogs & derivatives* / Q000097:blood; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism*; D010648:Phenylacetates / Q000097:blood*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives / Q000097:blood; D012701:Serotonin / Q000097:blood
D010648:Phenylacetates; D015102:3,4-Dihydroxyphenylacetic Acid; C005975:3,4-dihydroxyphenylethanol; C004277:hydroxyindoleacetaldehyde; D012701:Serotonin; D000431:Ethanol; D006897:Hydroxyindoleacetic Acid; C007430:3,4-dihydroxyphenylacetaldehyde; D000079:Acetaldehyde; D010626:Phenylethyl Alcohol; D004221:Disulfiram; D004298:Dopamine