Evaluation of permeability enhancement of hydrophilic compounds and macromolecular compounds by bile salts through rabbit corneas in-vitro.
The effect of bile salts, sodium taurocholate (TC-Na) and sodium taurodeoxycholate (TDC-Na), on the permeability of hydrophilic compounds and macromolecular compounds through the rabbit cornea in-vitro was examined. 6-Carboxyfluorescein and glutathione were used as low molecular weight hydrophilic model compounds and FITC-dextran (mol. wt 4000) and insulin were used as relatively macromolecular model compounds. TC-Na (2 and 10 mM) marginally increased the corneal permeabilities to hydrophilic compounds and macromolecular compounds. TDC-Na (2 and 10 mM) markedly increased the corneal permeabilities of these compounds.
D000818:Animals; D003315:Cornea / Q000187:drug effects / Q000378:metabolism*; D003840:Deoxycholic Acid / Q000031:analogs & derivatives*; D003911:Dextrans / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives*; D005452:Fluoresceins / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism; D008970:Molecular Weight; D010539:Permeability; D011817:Rabbits; D013656:Taurocholic Acid / Q000494:pharmacology*; D013657:Taurodeoxycholic Acid / Q000494:pharmacology*
D003911:Dextrans; D005452:Fluoresceins; D007328:Insulin; C015219:fluorescein isothiocyanate dextran; D003840:Deoxycholic Acid; C024098:6-carboxyfluorescein; D013657:Taurodeoxycholic Acid; D013656:Taurocholic Acid; D005978:Glutathione; D016650:Fluorescein-5-isothiocyanate