Effect of ruthenium red upon Ca2+ and Mn2+ uptake in Saccharomyces cerevisiae. Comparison with the effect of La3+.
The initial rate of both Ca2+ and Mn2+ uptake is inhibited by ruthenium red to about the same extent as by equivalent concentrations of La3+. The inhibition of Ca2+ uptake, however, is relieved during further incubation with ruthenium red. On preincubating the cells with ruthenium red even a stimulation of divalent cation uptake can be found. Relieve of the inhibition of divalent cation uptake is accompanied by K+ efflux. Both ruthenium red and La3+ displace Ca2+ very effectively from binding sites at the cell surface. The inhibition of initial Ca2+ uptake is accompanied by a reduction in the binding of Ca2+.
D002118:Calcium / Q000378:metabolism*; D002132:Calcium Radioisotopes; D002412:Cations; D007700:Kinetics; D007811:Lanthanum / Q000494:pharmacology*; D008345:Manganese / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D012428:Ruthenium / Q000494:pharmacology*; D012430:Ruthenium Red / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*
D002132:Calcium Radioisotopes; D002412:Cations; D012430:Ruthenium Red; D008345:Manganese; D007811:Lanthanum; D012428:Ruthenium; D011188:Potassium; D002118:Calcium