[Development of a method for the quantitative determination of Bordetella pertussis toxin and the prospects of its use for diagnosis].
Two variants of the enzyme immunoassay (EIA) systems for the determination of B. pertussis toxin (BPT), the "double sandwich" system and the competitive assay system, have been developed. For the titration of BPT in B. pertussis antigens the use of fetuin as the affinity base is preferable, and not antibodies from different paired animals. Of the two variants, the competitive EIA is more promising for diagnostic purposes.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology; D001886:Bordetella pertussis / Q000276:immunology; D002648:Child; D005069:Evaluation Studies as Topic; D006041:Goats / Q000276:immunology; D006386:Hemagglutination Tests; D006801:Humans; D007114:Immunization; D007117:Immunization, Secondary; D007124:Immunoenzyme Techniques; D037342:Pertussis Toxin; D011817:Rabbits; D010566:Virulence Factors, Bordetella / Q000032:analysis*; D014917:Whooping Cough / Q000175:diagnosis*; D000509:alpha-Fetoproteins
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D010566:Virulence Factors, Bordetella; D000509:alpha-Fetoproteins; D037342:Pertussis Toxin
Razrabotka metoda kolichestvennogo opredeleniia toksina kokliushnykh bakteriĭ i perspektivy ego ispol'zovanie dlia diagnostiki.