A simple silver-staining technique for detecting Bence Jones proteins in unconcentrated urine.
Detection of Bence Jones proteins in urine usually involves a concentration step, followed by electrophoresis and, if necessary, immunofixation. The time-consuming and expensive concentration step can be eliminated by use of the silver-stain technique described here. This procedure, routinely used for staining unconcentrated urine, is inexpensive, sensitive, and easily performed in a clinical laboratory. Bence Jones proteins can be detected in concentrations as low as 5 mg/L.
D001536:Bence Jones Protein / Q000652:urine*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008722:Methods; D012834:Silver; D013194:Staining and Labeling
D012834:Silver; D001536:Bence Jones Protein