A method for the direct measurement of glycogen synthase activity on gels after polyacrylamide gel electrophoresis.
A method for the detection of glycogen synthase activity after nondenaturing polyacrylamide gel electrophoresis is described. After the electrophoretical run, the gels were incubated in situ with UDP-glucose and glycogen. Labeled or unlabeled UDP-glucose could be used, since similar activity patterns were obtained by autoradiography or iodine staining of the gels. The method here described offers several advantages in terms of speed, sensitivity, and economy when compared with other procedures.
D000818:Animals; D001345:Autoradiography; D004591:Electrophoresis, Polyacrylamide Gel; D006006:Glycogen Synthase / Q000032:analysis*; D007455:Iodine; D009206:Myocardium / Q000201:enzymology; D051381:Rats; D012996:Solutions / Q000032:analysis; D013194:Staining and Labeling; D013997:Time Factors; D000516:alpha-Amylases
D012996:Solutions; D007455:Iodine; D006006:Glycogen Synthase; D000516:alpha-Amylases