Identification of a transcript and its promoter region on the archaebacterial plasmid pME2001.
The cryptic multicopy plasmid pME2001 of Methanobacterium thermoautotrophicum Marburg encodes a 611-base-pair transcript containing several consecutive, short open reading frames. Scrutiny of the 5'-flanking region did not reveal homology to putative archaebacterial consensus promoter sequences. However, 28 base pairs upstream of the transcription start point, there was a sequence with strong homology to a sequence preceding the purE gene of M. thermoautotrophicum.
D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D019605:Euryarchaeota / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012329:RNA, Bacterial / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D012329:RNA, Bacterial