title: Blockage of chloride channels by HEPES buffer.
abstract: Chloride channels of neurons of Drosophila are blocked when the cytoplasmic side of the membrane is exposed to the commonly used buffering agents 4-(2-hydroxyethyl)-1-piperazine ethanesulphonic acid (HEPES) and 4-morpholinepropanesulphonic acid (MOPS). In the presence of these compounds, chloride channels appear to function as a complex of multiple protochannels.
mesh_terms: D000818:Animals; D002712:Chlorides / Q000378:metabolism*; D004330:Drosophila; D006531:HEPES / Q000494:pharmacology*; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects; D009431:Neural Conduction / Q000187:drug effects; D009474:Neurons / Q000502:physiology; D010879:Piperazines / Q000494:pharmacology*
chemical_list: D002712:Chlorides; D007473:Ion Channels; D010879:Piperazines; D006531:HEPES