Transforming potential of the v-myb oncogene from avian myeloblastosis virus: alterations in the oncogene product may reveal a new target specificity.
Transfection of brown leghorn chicken embryo fibroblasts by DNA containing v-myb sequences cloned either in a complete AMV proviral DNA or in a retroviral derived vector has led to the isolation of two kinds of transformed cells. A characterization of the proviral sequences retained and expressed in these transformed cells revealed that they contained either new or altered v-myb-related RNA species. The experiments presented in this paper also show that both types of transformants expressed truncated myb-related polypeptides, suggesting that alterations of the v-myb product may result in a new target specificity, leading to the transformation of chicken embryo fibroblasts.
D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics*; D009189:Avian Myeloblastosis Virus / Q000235:genetics*; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002642:Chick Embryo; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D004274:DNA, Recombinant / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology; D005796:Genes; D005814:Genes, Viral; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000235:genetics; D009857:Oncogenes; D010455:Peptides / Q000302:isolation & purification; D012313:RNA / Q000302:isolation & purification; D014162:Transfection
D004274:DNA, Recombinant; D009363:Neoplasm Proteins; D010455:Peptides; D012313:RNA; D004247:DNA