Monoclonal anti-alpha (1----3) dextran antibodies of Igha BALB/c and Ighb C.B20 mice display striking similarities.
Allotype Ighb congenic C.B20 mice when immunized with dextran B1355S are unable to produce anti-alpha (1----3) dextran antibodies that express the VH-associated cross-reactive IdX idiotype. This intrastrain-specific idiotype is normally associated only with the anti-dextran response of Igha mice of which BALB/c is a prototype strain. In this study we have obtained monoclonal hybridoma antibodies specific for the alpha (1----3) glucosidic linkage of dextran from C.B20 mice that were presensitized with rabbit anti-IdX antibodies. These antibodies display the light chain isotype distribution, the H chain amino terminal sequence, share VH-associated IdX idiotypic determinants, and finally the similar fine specificity for dextrans observed for anti-alpha (1----3) dextran antibodies of BALB/c mice.
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics*; D000918:Antibody Specificity; D003911:Dextrans / Q000276:immunology*; D007126:Immunoglobulin Allotypes; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007526:Isoelectric Point; D051379:Mice; D008815:Mice, Inbred Strains / Q000276:immunology*
D000911:Antibodies, Monoclonal; D003911:Dextrans; D007126:Immunoglobulin Allotypes; D007143:Immunoglobulin Heavy Chains