Modulation of carcinoembryonic antigen release by HT-29 colon carcinoma line in the presence of different agents.
In this study we followed the effects of various differentiating agents on the expression of carcinoembryonic antigen (CEA) released into the medium by a colon carcinoma cell line HT-29. Butyric acid 1 mM markedly increased the level of CEA (12-fold in comparison to control levels). 12-O-tetradecanoyl-phorbol-13-acetate (TPA) 50 ng/ml and 5-azacytidine 4 x 10(-6) M increased the amount of CEA, 2- and 1.5-fold respectively. On the other hand retinoic acid 10(-5) M, N methyl-formamide 1% and N,N hexamethylene bisacetamide 2.5 mM decreased CEA 2-, 4- and 3-fold respectively. Our results emphasize that various differentiating agents affect CEA levels differently. Thus changes in CEA levels appear not to be reliable as a marker of a more differentiated phenotype.
D000081:Acetamides / Q000494:pharmacology; D001374:Azacitidine / Q000494:pharmacology; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002272:Carcinoembryonic Antigen / Q000032:analysis*; D002454:Cell Differentiation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000276:immunology*; D004357:Drug Synergism; D005559:Formamides / Q000494:pharmacology; D006801:Humans; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology
D000081:Acetamides; D002087:Butyrates; D002272:Carcinoembryonic Antigen; D005559:Formamides; D020148:Butyric Acid; D014212:Tretinoin; C014026:hexamethylene bisacetamide; D001374:Azacitidine; D013755:Tetradecanoylphorbol Acetate; C002950:methylformamide