Temperature-dependence of cytotoxicity of several genotoxicants in Chinese hamster V79 cells: bleomycin, paraquat, and some N-alkyl-N-nitrosoureas.
Chinese hamster V79 cultured cells were treated with bleomycin (BLM), paraquat (PQ), N-methyl-N-nitrosourea (MNU), and ACNU (an anti-cancer agent) at 30-43 degrees C. The survival fractions normalized by those released from heat-death were plotted vs. the integrated doses of the chemical. Arrhenius analysis of the cell inactivation by the chemical was made. The results revealed that BLM and PQ exerted synergistic cytotoxicity with hyperthermia, whereas MNU and ACNU exerted temperature-dependent cytotoxicities obeying the Arrhenius law.
D000818:Animals; D001761:Bleomycin / Q000633:toxicity*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D008433:Mathematics; D008770:Methylnitrosourea / Q000633:toxicity*; D015376:Nimustine; D009607:Nitrosourea Compounds / Q000633:toxicity*; D010269:Paraquat / Q000633:toxicity*; D013696:Temperature
D009607:Nitrosourea Compounds; D015376:Nimustine; D001761:Bleomycin; D008770:Methylnitrosourea; D010269:Paraquat