Eosin Y: a reversible stain for detecting electrophoretically resolved protein.
A method for the rapid staining of polypeptides in low-pH, urea-containing polyacrylamide gels is described. After 30 s of immersion in an alkaline solution of eosin Y, a variety of proteins stained with intensities similar to those seen with Coomassie R-250. By subjecting eosin-stained bands to electrophoretic elution, the eosin-protein complex was dissociated. This allowed for recovery of milligram quantities of electrophoretically resolved, stain-free protein. High recoveries of total protein as well as enzymatic activity were achieved using hen egg white lysozyme as a model protein.
D000818:Animals; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D004801:Eosine Yellowish-(YS); D009113:Muramidase / Q000032:analysis; D009504:Neutrophils / Q000032:analysis; D010455:Peptides / Q000097:blood; D011506:Proteins / Q000032:analysis*; D011817:Rabbits; D012394:Rosaniline Dyes; D013194:Staining and Labeling; D014508:Urea
D010455:Peptides; D011506:Proteins; D012394:Rosaniline Dyes; C024757:Coomassie brilliant blue R; D014508:Urea; D009113:Muramidase; D004801:Eosine Yellowish-(YS)