Na fluxes in human mononuclear leucocytes.
Unidirectional 22Na fluxes were investigated in human peripheral mononuclear leucocytes. Ouabain inhibited about 60% of Na efflux and the addition of bumetanide further reduced Na efflux rate by about 45%, suggesting the presence of a transport pathway capable of extruding Na against its gradient. Prostaglandins E1 and E2 and exogenous cAMP were found to be potent inhibitors of the bumetanide-sensitive Na efflux without affecting the ouabain-sensitive or the ouabain and bumetanide resistant Na effluxes.
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D003994:Bucladesine / Q000494:pharmacology; D002034:Bumetanide / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology; D011458:Prostaglandins E / Q000494:pharmacology; D012964:Sodium / Q000097:blood*; D012979:Sodium Radioisotopes
D011458:Prostaglandins E; D012979:Sodium Radioisotopes; D002034:Bumetanide; D010042:Ouabain; D003994:Bucladesine; D012964:Sodium; D015056:1-Methyl-3-isobutylxanthine