Effects of prolonged exposure to ammonia on fluid-phase, receptor-mediated, and adsorptive (non specific) endocytosis in cultured neuroblastoma cells. A flow-cytometry and cytochemical study.
The effect of prolonged exposure to ammonia on fluid-phase, receptor-mediated, and adsorptive (non specific) endocytosis in cultured neuroblastoma (Neuro-2a) cells were studied using fluorescein-labeled dextran, concanavalin A conjugated with fluorescein isothiocyanate, and cationized ferritin as tracers. Ammonia treatment increased the rate of endocytosis of cationized ferritin as well as the number of cell elements involved in the process. Moreover, the number of cytoplasmic components containing acid phosphatase activity was also found to increase following ammonia treatment. In contrast, flow-cytometric analyses showed that, under experimental conditions, exposure to ammonia did not alter the intralysosomal pH and had little effect on the fluid-phase and receptor-mediated endocytosis of fluorescein-labeled dextran and concanavalin-A fluorocrome, respectively.
D000110:Acetylcholinesterase / Q000032:analysis; D000135:Acid Phosphatase / Q000032:analysis; D000641:Ammonia / Q000633:toxicity*; D000818:Animals; D003208:Concanavalin A / Q000031:analogs & derivatives; D003911:Dextrans; D004705:Endocytosis / Q000187:drug effects*; D005293:Ferritins; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives*; D005452:Fluoresceins; D006651:Histocytochemistry; D047348:Hydrolyzable Tannins / Q000494:pharmacology; D009447:Neuroblastoma; D013997:Time Factors; D014407:Tumor Cells, Cultured
D003911:Dextrans; D005452:Fluoresceins; D047348:Hydrolyzable Tannins; C042784:fluorescein isothiocyanate-concanavalin A; C052526:fluorescein-dextran; C010297:polycationic ferritin; D003208:Concanavalin A; D000641:Ammonia; D005293:Ferritins; D000110:Acetylcholinesterase; D000135:Acid Phosphatase; D016650:Fluorescein-5-isothiocyanate