Detection and purification of rat and goat immunoglobulin G antibodies using protein G-based solid-phase radioimmunoassays.
Using the newly described streptococcal surface protein, protein G, which has powerful immunoglobulin G binding properties, solid-phase radioimmunoassays were developed for the quantitation of goat and rat immunoglobulin G bound to the plastic surface of microtiter plates. The binding of goat immunoglobulin G to the surface via a specific antigen (guinea pig alpha 1-microglobulin) permitted the determination of antigen-specific antibodies with a detection limit of 50-100 ng. Optimum assay conditions were established and the whole assay procedure could be brought to completion at room temperature in less than a working day. The value of the assays was illustrated by monitoring rat and goat immunoglobulin G antibodies during their purification from whole sera by classical chromatographic procedures.
D000510:Alpha-Globulins / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000032:analysis; D006041:Goats / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis* / Q000302:isolation & purification; D007163:Immunosorbent Techniques; D009419:Nerve Tissue Proteins; D011863:Radioimmunoassay; D051381:Rats; D013696:Temperature
D000510:Alpha-Globulins; D000906:Antibodies; C029152:G-substrate; D007074:Immunoglobulin G; D009419:Nerve Tissue Proteins; C016001:alpha-1-microglobulin