[Kinetics of the thermotolerance of human tongue squamous cell carcinoma cells (Tca 8113) in vitro].
This paper reports an observation on kinetics of induction and resolution of the thermotolerance expressed by Tca8113 in vitro in form of colony-forming rate, synchronization and laser flow cytometry after the cells being exposed to continuous and fractionized heat treatment of 41.5-45.5 degrees C for 4 hours. The process of thermotolerance kinetics could be divided into three phases: triggering, development and decay. The triggering phase lasted about 8 hours. The duration of development and decay phases was related to the temperature of initial heating. The higher the temperature, the shorter the duration. Generally, thermotolerance gradually disappeared 96 hours after the initial heating treatment. The thermotolerance of the synchronized cells in various phases was G1 greater than G2 greater than M greater than S. Hyperthermia simultaneously combined with pingyangmycin both produces a greater synergetic cytotoxic effect and reduces the degree of thermotolerance.
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D001761:Bleomycin / Q000494:pharmacology / Q000031:analogs & derivatives; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002453:Cell Cycle; D002460:Cell Line; D006358:Hot Temperature; D006801:Humans; D014062:Tongue Neoplasms / Q000473:pathology*
D000903:Antibiotics, Antineoplastic; D001761:Bleomycin; C021771:zhengguangmycin