Oxidized low density lipoprotein induces ceroid accumulation by murine peritoneal macrophages in vitro.
The presence of ceroid pigment inclusions within macrophage foam cells is a well-recognized phenomenon in human atherosclerosis but its significance is unclear. Murine peritoneal macrophages, maintained in a medium containing 10% lipoprotein-deficient fetal calf serum to which oxidized low density lipoprotein (LDL) has been added, rapidly accumulate similar ceroid inclusions. However, cells exposed to LDL, acetyl--LDL or dextran sulphate--LDL complexes fail to develop ceroid under the same culture conditions. Oxidation of LDL results in considerable physico-chemical changes to both protein and lipid moieties, and these may be important in the uptake of the particles by macrophages and, subsequently, the accumulation of intracellular ceroid.
D000818:Animals; D001161:Arteriosclerosis / Q000209:etiology; D001693:Biological Transport, Active; D002566:Ceroid / Q000378:metabolism*; D016264:Dextran Sulfate; D003911:Dextrans / Q000494:pharmacology; D066298:In Vitro Techniques; D008077:Lipoproteins, LDL / Q000378:metabolism / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010084:Oxidation-Reduction; D010529:Peritoneal Cavity / Q000166:cytology; D010860:Pigments, Biological / Q000378:metabolism*
D002566:Ceroid; D003911:Dextrans; D008077:Lipoproteins, LDL; D010860:Pigments, Biological; C032629:acetyl-LDL; D016264:Dextran Sulfate