Reduced electrogenic sodium-potassium pump in arterioles during renovascular hypertension.
The goal of this study was to assess the role of the electrogenic Na+-K+ pump in controlling active tone in cremasteric arterioles of normotensive hamsters and hamsters with bilateral (two-kidney, two figure-8) Grollman hypertension. Arterioles of both groups exhibited a large transient dilation when the Na+-K+ pump was stimulated by superfusing the cremaster muscle with physiological salt solution containing 15 mM K+ after 20 minutes of 0 mM K+ superfusion. Arteriolar dilation in response to 15 mM K+ was significantly smaller in the hypertensive animals than in sham-operated controls. Ouabain (10(-5) M and 10(-3) M) inhibited arteriolar dilation in response to 15 mM K+ in both groups of animals. Resting diameters, total active tone (assessed by application of 10(-4) M adenosine), and arteriolar responses to inhibition of the Na+-K+ pump by superfusion with 0 mM K+ or ouabain were not significantly different in normotensive and hypertensive animals. These data indicate that an electrogenic Na+-K+ pump can regulate active tone in cremasteric arterioles, and that the maximum response of this pump to stimulation with 15 mM K+ is reduced in arterioles of hamsters with two-kidney Grollman hypertension.
D000241:Adenosine / Q000494:pharmacology; D000818:Animals; D001158:Arteries / Q000378:metabolism*; D001160:Arterioles / Q000187:drug effects / Q000378:metabolism*; D006224:Cricetinae; D004594:Electrophysiology; D006978:Hypertension, Renovascular / Q000378:metabolism* / Q000503:physiopathology; D007473:Ion Channels / Q000502:physiology*; D008297:Male; D008647:Mesocricetus; D009994:Osmolar Concentration; D010042:Ouabain / Q000494:pharmacology; D010477:Perfusion; D011188:Potassium / Q000378:metabolism* / Q000494:pharmacology; D012146:Rest; D012964:Sodium / Q000378:metabolism*; D013779:Tetrodotoxin / Q000494:pharmacology
D007473:Ion Channels; D013779:Tetrodotoxin; D010042:Ouabain; D012964:Sodium; D000241:Adenosine; D011188:Potassium