Bleomycin resistance conferred by a drug-binding protein.
The protein coded by a bleomycin-resistance gene (ble) cloned from producing actinomycetes was purified from a culture of a recombinant E. coli strain and its action on bleomycin was determined by in vitro assays. The protein binds reversibly in a one to one ratio to bleomycin which can no longer cleave DNA. The bleomycin resistance of cells harboring a ble gene could be accounted for by a sequestering effect of the bleomycin-binding protein.
D000192:Actinomycetales / Q000235:genetics; D001761:Bleomycin / Q000378:metabolism* / Q000494:pharmacology; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism*; D004274:DNA, Recombinant; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005453:Fluorescence; D009994:Osmolar Concentration
D002352:Carrier Proteins; D004274:DNA, Recombinant; D001761:Bleomycin; D004247:DNA