gamma-Aminobutyric acid stimulates chloride permeability across microdissected Deiters' neuronal membrane.
Fluxes of 36Cl- across freshly prepared Deiters' neuronal membranes have been studied in a two-compartment microchamber simulating the extra- and the intracellular space. The rate of 36Cl- influx was enhanced by gamma-aminobutyric acid (GABA) (10(-4) M), the effect being reversed by picrotoxin (10(-4) M) and by bicuculline (10(-5) M). Diazepam (10(-8)-10(-7) M) did not potentiate the response to GABA and rather depressed it. However, a barbiturate site is most probably present in the GABA receptor complexes since pentobarbitone (10(-4) M) was able to stimulate 36Cl- permeability to the same extent as GABA itself.
D000818:Animals; D002451:Cell Compartmentation / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects*; D002712:Chlorides / Q000378:metabolism*; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism; D007700:Kinetics; D008846:Micromanipulation; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D011817:Rabbits; D014722:Vestibule, Labyrinth / Q000166:cytology / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
D002712:Chlorides; D007473:Ion Channels; D005680:gamma-Aminobutyric Acid