[Detection of glucocorticoid receptors in leukemia cells by dexamethasone-induced cytolysis and [3H]-dexamethasone binding].
The presence of glucocorticoid receptors on the leukemic cells of 33 patients affected with acute lymphatic leukemia (ALL) and 6 patients affected with acute myeloic leukemia (AML) was investigated by dexamethasone-induced cytolysis and [3H] dexamethasone binding. The tests undertaken proved that after 20 hours of incubation 9 of 26 non-T-non-B-ALL (c-ALL and unclassified ALL) and 2 of AML were lysed with dexamethasone; blood lymphocytes and bone marrow leukocytes of healthy donors, however, were not affected. Non-T-non-B-ALL and AML were able to bind essentially more [3H] dexamethasone than T-ALL. There existed no correlation between dexamethasone binding and dexamethasone-induced cytolysis.
D002470:Cell Survival / Q000187:drug effects; D003907:Dexamethasone / Q000097:blood* / Q000494:pharmacology; D006801:Humans; D007945:Leukemia, Lymphoid / Q000097:blood*; D015470:Leukemia, Myeloid, Acute / Q000097:blood*; D011869:Radioligand Assay; D011965:Receptors, Glucocorticoid / Q000378:metabolism*
D011965:Receptors, Glucocorticoid; D003907:Dexamethasone
Nachweis von Glukokortikoidrezeptoren auf Leukämiezellen durch Dexamethason-induzierte Zytolyse und [3H]-Dexamathasonbindung.