Nature and antigen-specific activities of transfer factor against herpes simplex virus type 1.
Transfer factor specific for herpes simplex virus (HSV) type 1 (TFHSV-1) was prepared from splenic cells of HSV-1 immunized mice. Protection was transferred with TFHSV-1 to nonimmune mouse recipients. The TFHSV-1 injected mice had a higher survival rate after lethal HSV-1 challenge as compared to mice injected with a nonspecific transfer factor (P less than 0.05). 51Cr-labelled leukocyte adherence inhibition (51-Cr-LAI) test was used to demonstrate the specific activity of transfer factor in vitro. Only leukocytes incubated with TFHSV-1 exhibited significant adherence inhibition (P less than 0.01) to HSV-1 antigen, but not to control antigen. Specific activity component of TFHSV-1 (STFc) was separated by affinity adsorption with the antigen. Activity of STFc in 51Cr-LAI test was significantly higher than that of TFHSV-1 (P less than 0.01). Ratio activity of STFc in protective host immunity was 16 times as much as that of TFHSV-1. STFc was analysed by high performance liquid chromatography, thin layer chromatography and isoelectric focusing in the polyacrylamide gel. Results revealed that STFc appeared to be a polypeptide with a molecular weight of about 12,870 dalton.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D000939:Epitopes; D006561:Herpes Simplex / Q000276:immunology*; D007111:Immunity, Cellular; D007525:Isoelectric Focusing; D007957:Leukocyte Adherence Inhibition Test; D007962:Leukocytes / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D018139:Simplexvirus / Q000276:immunology*; D014165:Transfer Factor / Q000032:analysis / Q000276:immunology*
D000596:Amino Acids; D000956:Antigens, Viral; D000939:Epitopes; D014165:Transfer Factor