title: The physicochemical properties and the process of pyrogel formation of IgD pyroglobulin.
abstract: The extremely rare IgD pyroglobulin caused opalescence, lost its antigenicity and acquired more negative charge when heated for 30 min at 56 degrees C. Exposure to 60 degrees C for 60 min produced a firm gel. The thermoprecipitability was abolished by the treatment with guanidine, urea and sodium dodecyl sulfate, but not by 2-mercaptoethanol or neuraminidase, and required the presence of both heavy and light chains. However, amino acid analysis showed abnormalities only in the heavy chain. We hypothesize that the irreversible heat-induced aggregation of IgD pyroglobulin is the result of a conformational change, triggered by light chain, which increases the molecular hydrophobicity.
mesh_terms: D000368:Aged; D000596:Amino Acids / Q000032:analysis; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D000939:Epitopes / Q000276:immunology; D006358:Hot Temperature; D006801:Humans; D007122:Immunoelectrophoresis; D007072:Immunoglobulin D; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D008297:Male; D009101:Multiple Myeloma / Q000276:immunology; D010266:Paraproteins; D011487:Protein Conformation; D011750:Pyroglobulins; D013997:Time Factors
chemical_list: D000596:Amino Acids; D000939:Epitopes; C051043:IgD pyroglobulin; D007072:Immunoglobulin D; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D010266:Paraproteins; D011750:Pyroglobulins; C013230:pyrogel