Local injection of cysteamine into the rat striatum decreases number and intensity of staining of neurons by indirect NADPH diaphorase reaction.
Cysteamine (100 micrograms) markedly reduces the number (by about 60%) and intensity of staining of NADPH diaphorase-reactive neurons 6 h after local injection into the striatum. This effect was reversible (after 24 h) and was only observed when the indirect staining procedure was applied in which NADPH formed by endogenous malate dehydrogenase is used. However, no direct effect of cysteamine on the malate dehydrogenase reaction was found. The decrease in NADPH diaphorase activity parallels the previously reported cysteamine induced decrease in somatostatin contained in the same neurons and may point to a biochemical interrelation of somatostatin and NADPH diaphorase in these neurons.
D000818:Animals; D003342:Corpus Striatum / Q000201:enzymology*; D003543:Cysteamine / Q000494:pharmacology*; D006651:Histocytochemistry; D007267:Injections; D008297:Male; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D009474:Neurons / Q000201:enzymology*; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling
D003543:Cysteamine; D009247:NADH, NADPH Oxidoreductases; D009252:NADPH Dehydrogenase