Influence of nimodipine, verapamil and lanthanum on histamine release from human basophils: effect of pretreatment with sialidase.
Our previous studies suggest that the membrane content of sialic acid influences histamine release from human basophils by interfering with the transmembraneous calcium fluxes preceding histamine release. In this study we investigated a possible interaction between membrane sialic acid and the calcium channels, using the calcium antagonists nimodipine, verapamil and lanthanum. Anti-IgE-induced histamine release was inhibited by verapamil, nimodipine and lanthanum. When cells were pretreated with sialidase in order to remove sialic acid from the cell membrane, the inhibitory action of nimodipine was abolished, whereas the inhibition by verapamil or lanthanum was unaffected. This difference may be explained by the different mode of action of the calcium channel antagonists, and the results suggest an association between membrane sialic acid and the calcium channel.
D001491:Basophils / Q000187:drug effects / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D007811:Lanthanum / Q000494:pharmacology*; D009439:Neuraminidase / Q000494:pharmacology*; D009553:Nimodipine / Q000494:pharmacology*; D012794:Sialic Acids / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology*
D012794:Sialic Acids; D009553:Nimodipine; D007811:Lanthanum; D014700:Verapamil; D009439:Neuraminidase