title: Evidence for substance P as a functional neurotransmitter in guinea pig small intestinal mucosa.
abstract: We showed previously that electrical transmural stimulation (TS) of guinea pig jejunal mucosa in vitro released neurotransmitters from submucosal plexus neurons which caused alterations in ion transport. The present studies were performed to obtain information regarding the identity of the neurotransmitters. The addition of exogenous substance P (SP) to the serosal side of the tissue caused a transient increase in short-circuit current (Isc) which closely mimicked the response to TS. Both TS and SP caused net secretion of Cl- ions by stimulating movement toward the luminal side. Tetrodotoxin abolished the response to TS, inhibited approximately 70% of the response to SP but did not affect the response to urecholine, a cholinergic muscarinic agonist. In the presence of the muscarinic antagonist, atropine, Isc responses to both TS and SP were reduced suggesting that a portion of both responses was due to action on enteric nerves causing release of acetylcholine. Following desensitization of the tissue with supramaximal doses of SP the response to TS was significantly reduced but the response to urecholine was unchanged. In the presence of atropine, SP desensitization reduced the nerve-stimulated response by approximately 65%; treatment of tissue with SP antibodies reduced the response by approximately 55%. Under the same conditions Isc responses to histamine were unaltered. Our results suggest that both SP (or a structurally analogous neurotransmitter) and acetylcholine as well as additional unidentified neurotransmitter(s) are functionally important in the regulation of intestinal ion transport in guinea pig jejunum.
mesh_terms: D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001626:Bethanechol Compounds / Q000494:pharmacology; D002712:Chlorides / Q000378:metabolism; D004558:Electric Stimulation; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000502:physiology*; D007421:Intestine, Small / Q000294:innervation*; D008297:Male; D018377:Neurotransmitter Agents; D013373:Substance P / Q000276:immunology / Q000502:physiology*
chemical_list: D001626:Bethanechol Compounds; D002712:Chlorides; D018377:Neurotransmitter Agents; D013373:Substance P; D001285:Atropine