Regulation of concentrations of mRNA for amylase, trypsinogen I and chymotrypsinogen B in rat pancreas by secretagogues.
Regulation of pancreatic gene expression by the secretory stimulants cholecystokinin-pancreozymin, caerulein and pilocarpine was studied in the rat, by using cloned cDNA probes to quantify concentrations of specific mRNAs (amylase, trypsinogen I and chymotrypsinogen B). It is concluded that long-term pancreatic stimulation results in pre-translational regulation of secretory-protein gene expression, with a preferential accumulation of RNA transcripts encoding serine proteinases, compared with that for amylase.
D000681:Amylases / Q000235:genetics*; D000818:Animals; D002108:Ceruletide / Q000494:pharmacology; D002766:Cholecystokinin / Q000494:pharmacology; D002919:Chymotrypsinogen / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D010862:Pilocarpine / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D014362:Trypsinogen / Q000235:genetics*
D012333:RNA, Messenger; D010862:Pilocarpine; D002108:Ceruletide; D014362:Trypsinogen; D002766:Cholecystokinin; D002919:Chymotrypsinogen; D000681:Amylases