Voltage-dependent chloride channels with several substates in excised patches from mouse neuroblastoma cells.
Single channels in mouse neuroblastoma cells with a high conductance of about 400 pS were described using the patch-clamp technique in the inside-out configuration. The channels were selective for Cl- as compared to cations and exhibited a linear I-V relationship between +40 and -40 mV. These Cl- channels were voltage-dependent and were activated by both depolarizing and hyperpolarizing potential steps from 0 mV to 10-40 mV. They closed, becoming inactivated, in tens of milliseconds (for depolarization) up to tens of seconds (for hyperpolarization) after each potential step. The typical feature of Cl- channels described was the dissipation of their conductance into several substates during the course of individual recordings.
D000818:Animals; D002460:Cell Line; D002712:Chlorides / Q000378:metabolism*; D002999:Clone Cells; D004553:Electric Conductivity; D007473:Ion Channels / Q000502:physiology*; D008564:Membrane Potentials; D051379:Mice; D009447:Neuroblastoma
D002712:Chlorides; D007473:Ion Channels