title: Polyploidization and localisation of poly(A)+ RNA in the different cell types of the vitellogenic meroistic ovary of the fleshfly, Sarcophaga bullata.
abstract: The degree of polyploidization, the level of transcriptional activity and the volume of the different cell types present in the meroistic ovary of Sarcophaga bullata were measured during different vitellogenic stages. The nurse cells and the germinal vesicle exhibited very pronounced differences with regard to DNA content and mRNA synthesis, even though they are genetically identical. During the 4C stage (late vitellogenesis), we observed different degrees of polyploidy in follicle cells adjacent to the oocyte and those surrounding the nurse cells. Although the chromatin of the germinal vesicle is condensed into a karyosome, in situ hybridisation revealed the presence of transcriptional activity. The volume of the germinal vesicle, which contains only 4C DNA, is big enough to contain 2048C DNA. The meroistic ovary is a highly polarized differentiating system. Our results are discussed in the light of the fact that the polytrophic ovary is a miniature electrophoresis chamber.
mesh_terms: D000818:Animals; D004396:Coloring Agents; D004175:Diptera / Q000166:cytology*; D005260:Female; D010053:Ovary / Q000166:cytology; D011061:Poly A / Q000032:analysis* / Q000235:genetics; D011123:Polyploidy; D012313:RNA / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger; D012394:Rosaniline Dyes; D014819:Vitellogenins / Q000235:genetics
chemical_list: D004396:Coloring Agents; C007722:Feulgen stain; D012333:RNA, Messenger; D012394:Rosaniline Dyes; D014819:Vitellogenins; D011061:Poly A; D012313:RNA