Estimation of association constants of 42 monoclonal antibodies to human IgG epitopes using a fluorescent sequential-saturation assay.
We measured the association constant (Ka) for 42 murine monoclonal antibodies (MAbs) to human IgG epitopes. Included are antibodies, previously evaluated in an IUIS/WHO collaborative study, to various epitopes on the four subclasses of human IgG - IgG Fc, IgG Fab, kappa, and lambda - and to selected IgG allotopes. We used a sequential-saturation immunofluorescent assay and interactive computer program to determine the Ka by Scatchard analysis. Kas ranged from unmeasurably low by this method (approximately 10(6) L/M) to 3.8 X 10(9) L/M. Some class specific MAbs had large Ka differences for different subclasses and some subclass specific MAbs had large Ka differences for molecules of the same subclass but of different light-chain types.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007126:Immunoglobulin Allotypes / Q000276:immunology; D007074:Immunoglobulin G / Q000145:classification / Q000276:immunology*; D007147:Immunoglobulin Light Chains / Q000276:immunology; D007700:Kinetics
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G; D007147:Immunoglobulin Light Chains