Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens.
Seven murine monoclonal antibodies were prepared that react with human class II antigens. Four of them (HL-39, MEM-12, MEM-24G, and MEM-32B: react with a monomorphic determinant dependent on association of heavy and light chains of DR antigens, two others (HL-38 and HL-40) recognize a monomorphic determinant localized on the light chains of DR and DP antigens. The antibody HL-37 is directed against a determinant present on DQ1 and DQ3, but not DQ2 molecules; at least in the case of DQ1, the epitope recognized is located on the light chain.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification*; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D006681:HLA-D Antigens / Q000032:analysis*; D006682:HLA-DP Antigens / Q000032:analysis* / Q000276:immunology; D006683:HLA-DQ Antigens / Q000032:analysis* / Q000276:immunology; D006684:HLA-DR Antigens / Q000032:analysis* / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000276:immunology; D007147:Immunoglobulin Light Chains / Q000276:immunology; D051379:Mice; D009000:Monocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006681:HLA-D Antigens; D006682:HLA-DP Antigens; D006683:HLA-DQ Antigens; C051568:HLA-DQ1 antigen; C051569:HLA-DQ3 antigen; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D003101:Collodion