Studies on species cross-reactivity of hemopexin by use of monoclonal and polyclonal antibodies.
The extent of immunological cross-reactivity between hemopexins of four species (rat, human, rabbit and chicken) was assessed with four affinity purified polyclonal antibodies and three monoclonal antibodies using RIA, Western blotting and rocket immunoelectrophoresis. Neither the two monoclonal antibodies to rabbit hemopexin (Rb3D11 and Rb3H9), the monoclonal antibody (R4B3) to rat hemopexin nor any of the polyclonal antibodies showed shared antigenic determinants between avian and mammalian hemopexins as judged by RIA or rocket immunoelectrophoresis. Western blotting with polyclonal antibodies revealed some reactivity raising the possibility of a few shared, though distantly related, epitopes. Polyclonal antibodies, raised to the mammalian hemopexins cross-reacted to variable extents with the respective antigens by RIA, results paralleled by data obtained by Western blotting. Anti-rat monoclonal antibodies reacted only with rat hemopexin in Western blots and minimally with rabbit hemopexin in RIA. The anti-rabbit monoclonal antibodies recognized two distinct epitopes one of which is shared with human hemopexin and presumably highly conserved.
D000818:Animals; D000911:Antibodies, Monoclonal; D002645:Chickens; D003429:Cross Reactions; D000939:Epitopes; D006466:Hemopexin / Q000276:immunology*; D006801:Humans; D011817:Rabbits; D051381:Rats; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006466:Hemopexin