Ia antigen expressed by keratinocytes can be the molecule of antigen presentation in contact sensitivity.
Immune response-associated (Ia) antigens are important as molecules in antigen recognition by T cells. Keratinocytes can express Ia antigens in many inflammatory dermatoses and by action of gamma interferon (IFN-gamma). A keratinocyte cell line, Pam 212 cells, expressed Ia antigens at maintaining culture. They also expressed Ia antigens under the influence of IFN-gamma. Liposomes carrying molecules from trinitrophenylated-Pam cells did not stimulate lymphnode cells (LCs) of contact sensitivity; however, liposomes carrying a large amount of those molecules stimulated LCs. This stimulation was attributable to contaminated Ia antigens in those molecules. Liposomes with a small amount of those molecules or trinitrophenylated-proteins from Pam cells were prepared. Into them were inserted Ia antigen-rich proteins from Pam cells, spleen cells, IFN-gamma-treated Pam cells, or epidermal cells containing Langerhans cells. Thus prepared liposomes stimulated the LCs significantly. The Ia antigens obtained from IFN-gamma-treated Pam cells worked as well as Ia antigens from epidermal cells that contained Langerhans cells as the molecule of antigen presentation in contact sensitivity.
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D002460:Cell Line; D003877:Dermatitis, Contact / Q000276:immunology*; D000078404:Epidermal Cells; D004817:Epidermis / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D007371:Interferon-gamma / Q000494:pharmacology; D007633:Keratins; D008081:Liposomes / Q000008:administration & dosage; D008198:Lymph Nodes / Q000187:drug effects; D008213:Lymphocyte Activation
D000949:Histocompatibility Antigens Class II; D008081:Liposomes; D007633:Keratins; D007371:Interferon-gamma