A simultaneous analytical method for catecholamines, indoleamines, and related compounds in 11 rat brain regions.
A simple high-performance liquid chromatographic method that allows the determination of 10 biogenic amines and related compounds for crude brain extracts has been developed. The compounds that can be quantified comprise norepinephrine, epinephrine, 3,4-dihydroxyphenylacetic acid, 3-methoxy-4-hydroxyphenylglycol, dopamine, 5-hydroxyindole-3-acetic acid, homovanillic acid, 3-methoxytyramine, 5-hydroxytryptamine and tryptophan. The detection system involves a three electrode-coulometric determination followed by fluorometric determination. This method is highly selective and sensitive, and in the present study, the usefulness of this methodology was confirmed by applying it to the determination of the levels of these various substances in 11 rat brain regions.
D000818:Animals; D001679:Biogenic Amines / Q000032:analysis*; D001923:Brain Chemistry; D002395:Catecholamines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006719:Homovanillic Acid / Q000032:analysis; D006897:Hydroxyindoleacetic Acid / Q000032:analysis; D008297:Male; D008734:Methoxyhydroxyphenylglycol / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000032:analysis
D001679:Biogenic Amines; D002395:Catecholamines; D012701:Serotonin; D008734:Methoxyhydroxyphenylglycol; D006897:Hydroxyindoleacetic Acid; D006719:Homovanillic Acid