title: Interferon beta 2/B-cell stimulatory factor type 2 shares identity with monocyte-derived hepatocyte-stimulating factor and regulates the major acute phase protein response in liver cells.
abstract: One of the oldest and most preserved of the homeostatic responses of the body to injury is the acute phase protein response associated with inflammation. The liver responds to hormone-like mediators by the increased synthesis of a series of plasma proteins called acute phase reactants. In these studies, we examined the relationship of hepatocyte-stimulating factor derived from peripheral blood monocytes to interferon beta 2 (IFN-beta 2), which has been cloned. Antibodies raised against fibroblast-derived IFN-beta having neutralizing activity against both IFN-beta 1 and -beta 2 inhibited the major hepatocyte-stimulating activity derived from monocytes. Fibroblast-derived mediator elicited the identical stimulated response in human HepG2 cells and primary rat hepatocytes as the monocyte cytokine. Finally, recombinant-derived human B-cell stimulatory factor type 2 (IFN-beta 2) from Escherichia coli induced the synthesis of all major acute phase proteins studied in human hepatoma HepG2 and primary rat hepatocyte cultures. These data demonstrate that monocyte-derived hepatocyte-stimulating factor and IFN-beta 2 share immunological and functional identity and that IFN-beta 2, also known as B-cell stimulatory factor and hybridoma plasmacytoma growth factor, has the hepatocyte as a major physiologic target and thereby is essential in controlling the hepatic acute phase response.
mesh_terms: D000209:Acute-Phase Proteins / Q000096:biosynthesis*; D000818:Animals; D000906:Antibodies / Q000276:immunology; D006528:Carcinoma, Hepatocellular; D005347:Fibroblasts / Q000032:analysis; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007249:Inflammation / Q000503:physiopathology*; D007370:Interferon Type I / Q000494:pharmacology*; D015850:Interleukin-6; D007963:Leukocytes, Mononuclear / Q000032:analysis; D008099:Liver / Q000378:metabolism / Q000503:physiopathology*; D008113:Liver Neoplasms; D008114:Liver Neoplasms, Experimental; D009363:Neoplasm Proteins / Q000096:biosynthesis; D011506:Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D000209:Acute-Phase Proteins; D000906:Antibodies; D007370:Interferon Type I; D015850:Interleukin-6; D009363:Neoplasm Proteins; D011506:Proteins; D011994:Recombinant Proteins