title: Intermediate filaments and desmosomal plaque proteins in testicular seminomas and non-seminomatous germ cell tumours as revealed by immunohistochemistry.
abstract: Seminomas and non-seminomatous testicular germ cell tumours were studied for the presence of cytokeratin and vimentin filaments and desmosomes using immunohistochemical methods. In the majority of the classical seminomas and in seminomatous area of mixed tumours most tumour cells appeared to lack cytokeratin filaments. Some seminomas contained a focally variable proportion of cells exhibiting cytokeratin-positive structures while other cases contained only few seminoma cells with a well developed fibrillar cytokeratin network. Gel electrophoresis of cytoskeletal proteins from microdissected regions revealed cytokeratin polypeptides nos. 8 and 18 typical of simple epithelia. In one seminoma, however, all, or almost all, tumour cells contained cytokeratin filaments. This finding is in line with the assumption of transitional forms between seminoma and embryonal carcinoma. Despite the lack - or variable expression - of cytokeratin filaments most seminoma cells contained desmosomes, although often few in number and irregularly distributed at the circumference of the cells. Loosely arranged and often very sparse vimentin fibrils were found in many, but not all seminoma cells. Double label immunofluorescence microscopy suggested that the majority of desmosomes was associated with intermediate filaments of the vimentin type. In contrast, in carcinoma cells of malignant teratomas, in well differentiated epithelial cells of intermediate-type malignant teratomas and in trophoblastic cells present in trophoblastic-type malignant teratomas cytokeratin filament bundles as well as desmosomes were decorated. The arrangement and density of the cytokeratin filament skeleton and of desmosomes varied with degree of maturation of the tissue. The most regular distribution and intensive staining of cytokeratin filaments and desmoplakin was found in "mature" tissues. Vimentin was demonstrated in mesenchymal areas and stroma cells. The results show that seminomas are distinguished from most other germ cell and non-germ cell tumours by the presence of true desmosomes together with scanty vimentin filaments in most tumour cells. In addition, they indicate that seminoma cells can be heterogeneous in their cytoskeletal complement and may include cells with cytokeratin expression, indicative of a multipotential character of the initially transformed cell(s).
mesh_terms: D000293:Adolescent; D000328:Adult; D000906:Antibodies; D000936:Antigen-Antibody Complex; D003598:Cytoskeletal Proteins; D003599:Cytoskeleton / Q000648:ultrastructure*; D051180:Desmoplakins; D003896:Desmosomes / Q000648:ultrastructure*; D004407:Dysgerminoma / Q000473:pathology* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006801:Humans; D007382:Intermediate Filaments / Q000648:ultrastructure*; D007633:Keratins / Q000032:analysis*; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D008854:Microscopy, Electron; D013736:Testicular Neoplasms / Q000473:pathology* / Q000648:ultrastructure; D014746:Vimentin / Q000032:analysis*
chemical_list: D000906:Antibodies; D000936:Antigen-Antibody Complex; D003598:Cytoskeletal Proteins; C495294:DSP protein, human; D051180:Desmoplakins; D008565:Membrane Proteins; D014746:Vimentin; D007633:Keratins