A novel binding site for bile acids on human serum albumin.
Binding sites of bile acids on human serum albumin were studied using various probes: dansylsarcosine (site I probe), 7-anilinocoumarin-4-acetic acid (ACAA, site II probe), 5-dimethylaminonaphthalene-1-sulfonamide (DNSA, site III probe), cis-parinaric acid (probe for fatty acid binding site) and bilirubin. Bile acids competitively inhibited the binding of dansylsarcosine to human serum albumin whereas bile acids enhanced the binding of ACAA, DNSA, cis-parinaric acid and bilirubin. Considering the concentrations of bile acids required to inhibit the binding of dansylsarcosine to human serum albumin, the secondary binding site of bile acids may correspond to site I. Dissociation constants (Kd) of the primary binding sites of lithocholic and chenodeoxycholic acid to human serum albumin were approximately 0.2 and 4 microM, respectively, which was measured by equilibrium dialysis at 37 degrees C. All the bile acids and their sulfates and glucuronides inhibited the binding of chenodeoxycholic acid to human serum albumin. Lithocholic and chenodeoxycholic acid and their sulfates and glucuronides exhibited more inhibition than cholic acid and its conjugates. In conclusion, bile acids may bind to a novel binding site on human serum albumin.
D001647:Bile Acids and Salts / Q000097:blood*; D001663:Bilirubin / Q000097:blood; D001665:Binding Sites; D003374:Coumarins / Q000097:blood; D003619:Dansyl Compounds / Q000097:blood; D005231:Fatty Acids, Unsaturated / Q000097:blood; D005456:Fluorescent Dyes; D005965:Glucuronates / Q000097:blood; D006801:Humans; D007700:Kinetics; D019301:Oleic Acid; D009829:Oleic Acids / Q000097:blood; D012521:Sarcosine / Q000031:analogs & derivatives / Q000097:blood; D012709:Serum Albumin / Q000378:metabolism*
D001647:Bile Acids and Salts; D003374:Coumarins; D003619:Dansyl Compounds; D005231:Fatty Acids, Unsaturated; D005456:Fluorescent Dyes; D005965:Glucuronates; D009829:Oleic Acids; D012709:Serum Albumin; C037087:dansylsarcosine; C001026:5-dimethylaminonaphthalene-1-sulfonamide; D019301:Oleic Acid; C054056:7-anilinocoumarin-4-acetic acid; C013641:parinaric acid; D001663:Bilirubin; D012521:Sarcosine