Isolation of renin-rich rat kidney cells.
Enzymatic dispersion and density gradient (Percoll) sedimentation were used to isolate a population of renin-containing, granule-laden cells (density 1.067 g/ml) from rat kidney cortex. Using immunohistochemistry (light microscopy) and electron microscopy, we defined the presence and ability of these cells to store renin protein(s). A 1000 base pair rat renin complementary DNA was used to show that these cells express the renin gene. The reverse hemolytic plaque assay defined the functional properties of the renin-containing cell. The data are consistent with the postulated inverse relationship between calcium concentration and release of renin. Thus, we have isolated a population of functional rat kidney cells that synthesize, store, and release renin.
D000818:Animals; D004247:DNA / Q000235:genetics; D006462:Hemolytic Plaque Technique; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007606:Juxtaglomerular Apparatus / Q000032:analysis* / Q000166:cytology / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000032:analysis; D051381:Rats; D011921:Rats, Inbred WKY; D012083:Renin / Q000032:analysis* / Q000235:genetics
D012313:RNA; D004247:DNA; D012083:Renin