Binding of polycation DEAE-dextran to Chinese hamster ovary cells induces reversible Ca2+ influx and inhibits capping of concanavalin A acceptor proteins.
Binding of the polycation DEAE-dextran to the cell surface of HA-1 CHO cells caused a marked increase in 45Ca2+ exchange influx. The effect was fairly selective for Ca2+, undirectional (efflux was not increased) and was rapidly reversed by treatment with polyanion dextran sulfate. 45Ca2+ influx could not be stimulated by treatment with multivalent lectins or fibronectin. In addition to stimulating 45Ca2+ flux, DEAE-dextran inhibited the capping of concanavalin-A acceptor proteins. Inhibition of capping occurred over the same DEAE-dextran concentration range (20-200 micrograms/ml) which stimulated 45Ca2+ uptake, possibly implicating increased cellular [Ca2+] in the inhibition of concanavalin A acceptor protein capping in this cell type. The profound effect of DEAE-dextran on cellular Ca2+ uptake and the rapid reversal of the effect by dextran sulfate might make the polycation a useful agent for the induction of transient increases in cellular [Ca2+].
D019325:3-O-Methylglucose; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D003637:DEAE-Dextran / Q000378:metabolism* / Q000494:pharmacology; D003911:Dextrans / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D008757:Methylglucosides / Q000378:metabolism; D008920:Misonidazole / Q000378:metabolism; D010053:Ovary; D011940:Receptor Aggregation / Q000187:drug effects*; D011952:Receptors, Concanavalin A / Q000378:metabolism*; D012413:Rubidium / Q000378:metabolism; D013501:Surface-Active Agents / Q000494:pharmacology
D003911:Dextrans; D008757:Methylglucosides; D011952:Receptors, Concanavalin A; D013501:Surface-Active Agents; D019325:3-O-Methylglucose; D008920:Misonidazole; D003637:DEAE-Dextran; D012413:Rubidium; D002118:Calcium