T- and B-lymphocytes in pemphigus vulgaris. A clinico-immunological follow-up study of fifty patients with pemphigus vulgaris.
Peripheral blood T-lymphocytes, studied in 50 patients with pemphigus vulgaris by means of spontaneous (E) and active (EA) rosette-formation, were increased in active disease and gradually returned to normal after achieving clinical remission of pemphigus vulgaris. The portion of B-cells quantified by M-rosette formation was increased in active disease as well (19.9 +/- 3.0% vs. 10.8 +/- 1.6% in controls) (p less than 0.02) but returned to normal immediately after clinical remission was obtained. Levamisole in vitro suppressed E-, EA-, and M-rosette formation in all the patients examined with active disease. The subsets of T-cells in 10 non-treated patients with active pemphigus vulgaris and 5 patients with a long-term steroid maintained remission of the disease were studied using OKT series monoclonal antihuman antibodies. A statistically significant increase in the percentage of OKT3+ cells (p less than 0.05) and a considerable increase in OKT4+ cell count (p less than 0.002) were registered in patients with active pemphigus vulgaris, compared with controls. In five patients with 2-9 years' remission the percentage of OKT3+, OKT4+, OKT5+ and OKT8+ cells did not significantly differ from controls.
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001402:B-Lymphocytes / Q000145:classification*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007978:Levamisole / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D010392:Pemphigus / Q000188:drug therapy / Q000276:immunology*; D012397:Rosette Formation; D013256:Steroids / Q000627:therapeutic use; D013601:T-Lymphocytes / Q000145:classification* / Q000187:drug effects
D013256:Steroids; D007978:Levamisole