Differences in anionic dependence of the synaptic efflux of D-aspartic acid and gamma-aminobutyric acid.
The synaptosomal effluxes of D-aspartate and gamma-aminobutyric acid (GABA) induced by a substitution of the Cl- ions with propionate in the incubation medium were measured in preparations of hippocampal tissue homogenates. The efflux of aspartate was significantly higher than spontaneous efflux at 125 mM Cl- (normal = 144 mM) and was increased with decreasing Cl- concentration. GABA efflux was much less sensitive to a reduction in Cl- concentration than D-aspartate. The efflux was Ca2+-dependent in both cases.
D000818:Animals; D000838:Anions; D001224:Aspartic Acid / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D007473:Ion Channels / Q000502:physiology; D008297:Male; D011422:Propionates / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013574:Synaptosomes / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D000838:Anions; D002712:Chlorides; D007473:Ion Channels; D011422:Propionates; D001224:Aspartic Acid; D005680:gamma-Aminobutyric Acid; C029658:propionic acid; D002118:Calcium