[Fibronectin fragmentation unmasks the activity stimulating DNA and RNA biosynthesis in granulation tissue cells in vitro].
Human blood plasma fibronectin decreased slightly the incorporation of precursors into nucleic acids of granulation tissue culture cells. A slight fragmentation of fibronectin, where the fragments with 180-200 kD molecular mass were developed, led to occurrence of the activity 2-fold stimulating the DNA synthesis. After more effective proteolysis using plasmin and trypsin the stimulating effect of fibronectin fragments on synthesis of nucleic acids maintained and constituted 165 +/- 12% and 127 +/- 7% for DNA and RNA, respectively.
D000818:Animals; D002455:Cell Division; D004247:DNA / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D005353:Fibronectins / Q000302:isolation & purification / Q000502:physiology*; D006097:Granulation Tissue / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D008297:Male; D010446:Peptide Fragments / Q000502:physiology; D012313:RNA / Q000096:biosynthesis*; D051381:Rats
D005353:Fibronectins; D010446:Peptide Fragments; D012313:RNA; D004247:DNA
Fragmentirovanie fibronektina demaskiruet aktivnost', stimuliruiushchuiu sintez DNK i RNK v kletkakh granulatsionnoĭ tkani in vitro.