Cell components in proliferative vitreoretinopathy: immunofluorescent double staining of cultured cells from proliferative tissues.
Intermediate filament proteins in cultured cells from epiretinal membranes of 5 cases of proliferative vitreoretinopathy and subretinal strands of 2 cases were immunohistologically characterized. Double staining technique after colcemid treatment revealed that either keratin-positive or vimentin-positive cells were found with different ratios depending on the cases. Both positive and both negative cells were also observed. Desmin and glial fibrillary acidic protein were totally negative.
D000328:Adult; D000368:Aged; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007633:Keratins / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010857:Pigment Epithelium of Eye / Q000473:pathology; D011498:Protein Precursors / Q000378:metabolism; D012160:Retina / Q000473:pathology; D012164:Retinal Diseases / Q000473:pathology*; D014746:Vimentin / Q000378:metabolism; D014822:Vitreous Body / Q000473:pathology*
D011498:Protein Precursors; D014746:Vimentin; C004818:prekeratin; D007633:Keratins