[Primary structure of the beta-subunit of Na+,K+-ATPase from the swine kidney. II. Reverse transcription, cloning of mRNA, complete nucleotide sequence corresponding to the structural region of the gene].
mRNA coding for beta-subunit of Na+, K+-ATPase from pig kidney is about 24-25S as deduced from the hybridization pattern of poly (A)+-RNA with two synthetic oligonucleotides structurally corresponding to two peptides isolated from the tryptic hydrolyzate of beta-subunit. Cloning of cDNA allowed to determine the complete structure of the gene and to deduce the amino acid sequence of beta-subunit of Na+, K+-ATPase. The beta-subunit contains 302 amino acid residues and the protein is not processed at the N-nor at the C-terminus.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D005796:Genes; D006868:Hydrolysis; D007668:Kidney / Q000201:enzymology*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics*; D012194:RNA-Directed DNA Polymerase / Q000235:genetics*; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics*; D013552:Swine; D013698:Templates, Genetic; D014357:Trypsin
D012333:RNA, Messenger; D004247:DNA; D012194:RNA-Directed DNA Polymerase; D014357:Trypsin; D000254:Sodium-Potassium-Exchanging ATPase
Pervichnaia struktura beta-sub''edinitsy Na+,K+-ATP-azy pochek svin'i. II. Obratnaia transkriptsiia, klonirovanie mRNK, opredelenie polnoĭ nukleotiodnoĭ posledovatel'nosti, sootvetstvuiushcheĭ strukturnoĭ chasti gena.