title: Potential problems in serum protein electrophoresis.
abstract: Potential problems are described that one could encounter in carrying out an electrophoretic procedure including its ancillary phases of visualization (staining) and quantification (densitometry). Endpoint-like measurements for separated isoenzymes may provide artifactual kinetic values as well, because stain measurement is fixed at a single time whereas reagent blanking in the electrophoretic medium is substituted for the conventional serum initial absorbance readings of test-tube determinations. Truncation of separated electrophoretic zones or opacity of an electrophoretic anti-convection medium such as uncleared cellulose acetate may also interfere with absolute quantification procedures.
mesh_terms: D000418:Albumins / Q000032:analysis; D001797:Blood Protein Electrophoresis / Q000379:methods*; D001798:Blood Proteins / Q000032:analysis*; D003720:Densitometry / Q000379:methods; D005916:Globulins / Q000032:analysis; D006801:Humans; D008055:Lipids / Q000097:blood; D013194:Staining and Labeling
chemical_list: D000418:Albumins; D001798:Blood Proteins; D005916:Globulins; D008055:Lipids