On the functional roles of simian virus 40 large and small T-antigen in the induction of a mitotic host response.
The early gene of wild-type (wt) SV40 specifies two related proteins, referred to as large (Mr 88,000) and small (Mr 19,000) T-antigen. Infection with wt SV40 of Go/G1-arrested monkey kidney and CV-1 cell cultures induced in virtually 100% of the cells T-antigen synthesis, followed by a mitotic reaction and the production of SV40 DNA. Parallel cultures were infected with SV40 deletion mutants that produce either no small T-antigen (d1883) or only trace amounts of a truncated form (d1891). Kinetics of synthesis and accumulation of large T-antigen was closely similar to that observed with wtSV40 whereas apparently only 50-60% of the cells participated in the mitotic reaction and the production of viral DNA. These results and those obtained from a comparative study on the abortive (transforming) infection in Go-arrested mouse tissue culture cells indicate that synthesis of large T-antigen alone is sufficient to trigger in 50-60% of the infected cells a mitotic reaction.
D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor / Q000096:biosynthesis / Q000502:physiology*; D001345:Autoradiography; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D008938:Mitosis; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis / Q000502:physiology*; D011506:Proteins / Q000032:analysis; D012313:RNA / Q000032:analysis; D013539:Simian virus 40; D014412:Tumor Virus Infections / Q000378:metabolism
D000952:Antigens, Polyomavirus Transforming; D000957:Antigens, Viral, Tumor; D009856:Oncogene Proteins, Viral; D011506:Proteins; D012313:RNA; D004247:DNA