Production of Aujeszky vaccine by the microcarrier technology "from the ampoule to the 500 litre fermentor".
A process for the production of Aujeszky vaccine on microcarrier cultures in large fermentors is described. The NLST cells are routinely produced in fermentors of 150 litres; cell density reaches 0.7-0.8 10(6)/ml. The authors describe the method used to obtain those results. The different parameters used in fermentors are discussed and especially pO2 regulation. A study of subculturing method is being carried out to reach a volume of 500 litres. The comparison between stationary culture and microcarrier cultures shows the advantages of such a technique for the viral antigen produced versus infective and the increase of the bulk capacity. This experience also shows that specific development is required for each cell line.
D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis*; D001709:Biotechnology / Q000379:methods*; D002448:Cell Adhesion; D002478:Cells, Cultured; D003911:Dextrans; D005285:Fermentation; D011558:Herpesvirus 1, Suid / Q000254:growth & development / Q000276:immunology*; D013552:Swine; D014765:Viral Vaccines / Q000096:biosynthesis*; D014779:Virus Replication
D000956:Antigens, Viral; D003911:Dextrans; D014765:Viral Vaccines