[Demonstration of viral proteins by silver staining].
Investigations were conducted to establish the optimal conditions for silver staining of polypeptides separated by polyacryle-amide gel electrophoresis. The thickness of the gel layer was of 1.5 mm. The experiments showed that the modified Hankeshover and Dernick's technique (4) (replacement of the fixative for the electrophoretically separated polypeptides and extension of the bleaching by Farmer's reagent time) had the best sensitivity and reproducibility. This technique can be performed also on polyacryle-amide gel preparations already stained by the Coomassie Brilliant Blue method. To check the efficiency of this silver staining technique, the authors used it to detect the Sendai virus and B hepatitis virus surface antigen polypeptides.
D004591:Electrophoresis, Polyacrylamide Gel; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D010222:Parainfluenza Virus 1, Human / Q000032:analysis; D010455:Peptides / Q000032:analysis; D012394:Rosaniline Dyes; D012834:Silver; D013194:Staining and Labeling / Q000379:methods*; D014764:Viral Proteins / Q000032:analysis* / Q000302:isolation & purification
D006514:Hepatitis B Surface Antigens; D010455:Peptides; D012394:Rosaniline Dyes; D014764:Viral Proteins; D012834:Silver; C024757:Coomassie brilliant blue R
Mise en évidence des protéines virales à l'aide de la coloration par l'argent.