[Synthesis of monoclonal antibodies to tissue-specific antigens of human skin and thymus epithelium in polyclonal activation by pertussis toxin].
Following the immunization of BALB/c mice with B. pertussis toxin, monoclonal antibodies (McAb) to the antigens of human epithelium, both dermal (the basal, superbasal or all epidermis levels) and thymic (the epithelium of the medullary zone, the cortical and medullary epithelium around Hassall's corpuscles), have been obtained. McAb have been obtained as the result of the polyclonal activation of autoreactive B-cells with B. pertussis toxin. McAb thus obtained can be used for the determination of the corresponding antigens in the epithelial tissues of the thymus and other organs in man, as well as for the diagnosis of tumors, histogenetically related to integumentary tissues of the epidermal genesis.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000937:Antigen-Antibody Reactions; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D004848:Epithelium / Q000276:immunology; D000939:Epitopes / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D037342:Pertussis Toxin; D012867:Skin / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D010566:Virulence Factors, Bordetella; D037342:Pertussis Toxin
Sintez monoklonal'nykh antitel k tkanespetsificheskim antigenam épiteliia kozhi i timusa cheloveka pri poliklonal'noĭ aktivatsii kokliushnym toksinom.