Genome analysis and reverse transcriptase activity of human teratocarcinoma-derived retroviruses.
Five of five human teratocarcinomas cultured in vitro could be induced to synthesize retrovirus-like particles, albeit in extremely low amounts. The accumulation and purification of the human teratocarcinoma-derived retrovirus (HTDV) from one of these cell lines allowed characterization of its genomic material as high mol. wt. (60S) RNA. Partial purification of HTDV-associated RNA-dependent DNA polymerase (reverse transcriptase) has also been achieved. HTDVs are easily distinguishable from the exogenous human T-lymphotropic viruses and human immunodeficiency viruses by morphological, biological and immunological criteria.
D006801:Humans; D008854:Microscopy, Electron; D008970:Molecular Weight; D012367:RNA, Viral / Q000235:genetics*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D012190:Retroviridae / Q000201:enzymology / Q000235:genetics* / Q000648:ultrastructure; D018894:Reverse Transcriptase Inhibitors; D013698:Templates, Genetic; D013724:Teratoma / Q000382:microbiology*
D012367:RNA, Viral; D018894:Reverse Transcriptase Inhibitors; D012194:RNA-Directed DNA Polymerase