Identification of kallikrein in cultures of adult renal cells.
Cortical tubular cultures enriched with distal segments were prepared from the kidney of adult Fisher 344 rats bearing thyroid tumors. After two years of cultivation (20 passages) cell monolayers contained immunocytochemically detectable cytokeratin and kallikrein material in their cytoplasm. Furthermore cell pellets showed a true renal type of kininogenase activity corresponding to active kallikrein which ranged from 22.3 to 1.5, and total Kallikrein from 29.9 to 2.8 pg kinins/min per mg of protein.
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D007150:Immunohistochemistry; D007610:Kallikreins / Q000378:metabolism*; D007633:Keratins / Q000378:metabolism; D007684:Kidney Tubules / Q000378:metabolism*; D007686:Kidney Tubules, Distal / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344
D007633:Keratins; D007610:Kallikreins