[Use of a filtration technic for determining the content and metabolic rate of alkaline cations in cells by flame-emission analysis].
The filtration technique of rapid separating cells from the medium suitable for determination of intracellular cations simultaneously in 20-24 samples is described. The technique was used for estimating the constant rate of Rb+-K+ exchange in rat thymocytes by flame emission without radiotracers.
D000818:Animals; D002412:Cations / Q000032:analysis* / Q000378:metabolism; D002469:Cell Separation; D002477:Cells / Q000378:metabolism*; D003584:Cytological Techniques / Q000295:instrumentation; D005260:Female; D005374:Filtration / Q000295:instrumentation / Q000379:methods; D006863:Hydrogen-Ion Concentration; D007473:Ion Channels / Q000032:analysis / Q000378:metabolism; D008214:Lymphocytes / Q000032:analysis / Q000378:metabolism; D008297:Male; D051381:Rats; D013053:Spectrophotometry / Q000295:instrumentation / Q000379:methods*; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism
D002412:Cations; D007473:Ion Channels
Isplo'zovanie fil'tratsionnoĭ tekhniki dlia opredeleniia soderzhaniia i skorosti obmena shchelochnykh kationov v kletkakh s pomoshch'iu plamenno-émissionnogo analiza.