Fine specificity of human HLA-B7-specific cytotoxic T-lymphocyte clones. I. Identification of HLA-B7 subtypes and histotopes of the HLA-B7 cross-reacting group.
Three HLA-B7-specific cytotoxic T lymphocyte (CTL) clones are described. Their fine specificity is examined by extensive panel and family studies. Four CTL-defined subtypes of HLA-B7 could be found: HLA-B7.1, -B7.2, -B7.3, and -B7.4. Biochemical evidence was obtained in one-dimension isoelectric focusing for only two subtypes. The estimated subtype frequencies in the Caucasian population are presented. Furthermore, a linkage disequilibrium between HLA-A29 and HLA-B7.3 is suggested. The CTL clones exerted distinct "extra-reactivities", i.e., lysis of some non-B7 target cells. These extra reactivities could be assigned to the cross-reacting group of HLA-B7.
D002478:Cells, Cultured; D002999:Clone Cells / Q000276:immunology; D003429:Cross Reactions; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D000939:Epitopes / Q000032:analysis; D006680:HLA Antigens / Q000145:classification / Q000276:immunology*; D015793:HLA-B7 Antigen; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods; D007959:Lymphocyte Culture Test, Mixed / Q000379:methods; D010641:Phenotype; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000939:Epitopes; D006680:HLA Antigens; D015793:HLA-B7 Antigen