Reaction of antipolynucleotide antibody from systemic lupus erythematosus patient serum with double-stranded DNA complexed to protein.
Antipolynucleotide antibody from systemic lupus erythematosus patient serum was found to interact with common determinants of double-stranded DNA (dsDNA) complexed to protein within nucleosomes, and with protein-free denatured DNA. In contrast, purified dsDNA isolated from nucleosomes manifested decreased reactivity with antipolynucleotide antibody. These data suggest that immunoassays using denatured DNA antigen substrate are useful for identifying a broad spectrum of antipolynucleotide antibodies that are reactive with dsDNA complexed to protein.
D000974:Antibodies, Antinuclear / Q000276:immunology*; D000915:Antibody Affinity; D000937:Antigen-Antibody Reactions; D004247:DNA / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D011119:Polynucleotides / Q000276:immunology; D011863:Radioimmunoassay
D000974:Antibodies, Antinuclear; D000939:Epitopes; D011119:Polynucleotides; D004247:DNA