Effects of ATP and other nucleotides on DNA repair synthesis in bleomycin-pretreated permeable mouse sarcoma cells.
Effects of ATP and some other nucleotides (AMP, ADP, CTP, GTP, UTP and dATP) on reparative DNA synthesis and repair patch ligation in bleomycin-pretreated permeable mouse sarcoma cells were studied. Reparative DNA synthesis was significantly stimulated by 2.5 mM ATP, ADP or dATP. The stimulation was observed on both DNA polymerase alpha- and beta-dependent reparative DNA synthesis. ATP concentration required for repair patch ligation was much lower than that required for the stimulation of reparative DNA synthesis. An apparent Km value for ATP of the repair patch ligation was about 40 microM. ADP supported repair patch ligation after being converted into ATP by adenylate kinase in permeable cells.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000249:Adenosine Monophosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D001761:Bleomycin / Q000494:pharmacology*; D003570:Cytidine Triphosphate / Q000494:pharmacology; D004260:DNA Repair / Q000187:drug effects*; D004261:DNA Replication / Q000187:drug effects; D003838:Deoxyadenine Nucleotides / Q000494:pharmacology; D006160:Guanosine Triphosphate / Q000494:pharmacology; D051379:Mice; D008809:Mice, Inbred C3H; D009711:Nucleotides / Q000494:pharmacology*; D012513:Sarcoma, Experimental / Q000235:genetics*; D014544:Uridine Triphosphate / Q000494:pharmacology
D003838:Deoxyadenine Nucleotides; D009711:Nucleotides; D001761:Bleomycin; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D003570:Cytidine Triphosphate; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; C026600:2'-deoxyadenosine triphosphate; D014544:Uridine Triphosphate