Precipitation of LDL with sulfated polyanions: three methods compared.
Three precipitation methods for the determination of low density lipoproteins have been evaluated. In n = 113 normolipidemic samples mean LDL-cholesterol levels have been 2.90 mmol/l, 2.77 mmol/l, 3.21 mmol/l after precipitation with heparin, dextran sulfate, and polyvinylsulfate, respectively. As compared to a combined ultracentrifugation and precipitation reference procedure (mean 3.22 mmol/l) two precipitation methods tend to underrate LDL-cholesterol. Elevated plasma triglycerides may interfere with the precipitation of LDL. The clinical relevance of the precipitation procedures has been studied by discriminant analysis in n = 28 consecutive patients admitted for coronary bypass operation and n = 28 controls. The data suggest that, statistically, the determination of LDL-cholesterol with either precipitation method only provides redundant information as in relation to the Friedewald approximation for LDL-cholesterol. Immunologically determined apolipoprotein B proved a better predictor for group separation than either precipitation method.
Siekmeier
R
R
Gustav Embden-Zentrum der biologischen Chemie, J.W. Goethe-Universität Frankfurt/Main, FRG.
März
W
W
Gross
W
W
D000328:Adult; D011232:Chemical Precipitation; D003327:Coronary Disease / Q000097:blood; D016264:Dextran Sulfate; D003911:Dextrans; D005260:Female; D006493:Heparin; D006801:Humans; D007202:Indicators and Reagents; D008077:Lipoproteins, LDL / Q000097:blood*; D008297:Male; D008875:Middle Aged; D011145:Polyvinyls; D014461:Ultracentrifugation
D003911:Dextrans; D007202:Indicators and Reagents; D008077:Lipoproteins, LDL; D011145:Polyvinyls; C019050:polyvinyl sulfate; D006493:Heparin; D016264:Dextran Sulfate