A simple fixation and embedding method for use in hybridization histochemistry on plant tissues.
A simple method for fixing and embedding plant tissue for hybridization histochemistry has been developed. Material is fixed with paraformaldehyde-glutaraldehyde by the phase-partition technique and embedded in LR Gold. The method preserves nucleic acids in situ. Double-stranded 32P-labelled DNA probes hybridized to complementary RNAs in thin and semithin sections. Biotinylated probes detected with immunogold markers have been used to localize transcripts at the electron microscopic level.
McFadden
G I
GI
Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Victoria, Australia.
Bonig
I
I
Cornish
E C
EC
Clarke
A E
AE
D015342:DNA Probes / Q000032:analysis; D005404:Fixatives; D006651:Histocytochemistry / Q000379:methods*; D008854:Microscopy, Electron / Q000379:methods*; D009693:Nucleic Acid Hybridization; D010944:Plants / Q000378:metabolism*; D010947:Plants, Toxic; D012313:RNA / Q000032:analysis; D014026:Nicotiana
D015342:DNA Probes; D005404:Fixatives; D012313:RNA