Major structural polypeptides of retrovirus produced by a Suncus murinus mammary tumor cell line and type D retrovirus share interspecies antigenic determinants.
Constitutive polypeptides of retrovirus produced by a Suncus murinus mammary tumor cell line (Sm-MTV) were characterized by immunofluorescence and immunoblotting. The major structural polypeptide of Sm-MTV, molecular weight 44/43K, immunologically cross reacted with the structural polypeptides of Mason-Pfizer monkey virus (MPMV) (molecular weights 27K and 12/10K). Sm-MTV constitutive polypeptides did not show antigenic relatedness with those of the following mammalian retroviruses: murine mammary tumor virus, Rausher murine leukemia virus, Molony murine leukemia virus, Osborn-Mendel rat leukemia virus, feline leukemia virus, RD114 virus, simian sarcoma virus 1, and human T-cell leukemia virus type I. Among the Sm-MTV structural polypeptides, those with molecular weights of 72/69K and 47K were glycosylated.
Aoyama
A
A
Second Department of Pathology, Nagoya University School of Medicine, Japan.
Saga
S
S
Nagayoshi
S
S
Yokoi
T
T
Malavasi
J
J
Takenaka
T
T
Tsutsui
Y
Y
Hoshino
M
M
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D008321:Mammary Glands, Animal; D009369:Neoplasms / Q000662:veterinary; D010455:Peptides / Q000276:immunology*; D012190:Retroviridae / Q000276:immunology*; D012191:Retroviridae Proteins / Q000276:immunology*; D012788:Shrews; D014407:Tumor Cells, Cultured
D000956:Antigens, Viral; D000939:Epitopes; D010455:Peptides; D012191:Retroviridae Proteins