Application of an enzyme-linked immunosorbent-inhibition assay to quantitate the release of KS peptides into fluids of the rat subcutaneous air-pouch model and the effects of chondroprotective drugs on the release process.
The effects of the chondroprotective agents (Arteparon, SP-54 and DH40J) on the release of proteoglycan degradation products (as keratan sulphate peptide fragments) from articular cartilage implanted into rat subcutaneous air pouches have been investigated by using an enzyme-linked immunosorbent-inhibition assay (ELISIA). The ELISIA technique was capable of quantitating the keratan sulphate peptides (KS peptides) in fluids within the range of 100-2,000 ng/ml by using the monoclonal antibody line 1/20/5-D-4 and human articular cartilage KS peptides as standard reagents. It was found that the levels of KS peptides present in the air-pouch fluid of rats treated with the chondroprotective drugs was significantly less than in fluid aspirated from the pouches of non-drug-treated control animals. On the basis of these findings we suggest that the assessment of KS peptide by ELISIAs may provide a useful means of monitoring proteoglycan breakdown products in biological fluids (e.g. synovial fluids or blood) and for evaluating the effects that antiarthritic drugs may have on this process.
Kongtawelert
P
P
Raymond Purves Research Laboratories, University of Sydney, St. Leonards, N.S.W., Australia.
Francis
D J
DJ
Brooks
P M
PM
Ghosh
P
P
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001172:Arthritis, Rheumatoid / Q000503:physiopathology; D002358:Cartilage, Articular / Q000187:drug effects / Q000378:metabolism*; D011508:Chondroitin Sulfate Proteoglycans; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D006025:Glycosaminoglycans / Q000494:pharmacology; D007632:Keratan Sulfate; D000071234:Lumican; D008297:Male; D010003:Osteoarthritis / Q000503:physiopathology; D010426:Pentosan Sulfuric Polyester / Q000494:pharmacology / Q000031:analogs & derivatives; D010446:Peptide Fragments / Q000032:analysis / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D000894:Anti-Inflammatory Agents, Non-Steroidal; D011508:Chondroitin Sulfate Proteoglycans; D006025:Glycosaminoglycans; C000604306:LUM protein, human; C000604308:Lum protein, rat; D000071234:Lumican; D010446:Peptide Fragments; C059643:zinc-chelated pentosan polysulfate; D010426:Pentosan Sulfuric Polyester; C010034:Arteparon; D007632:Keratan Sulfate