The reliability of urease tests, histology and culture in the diagnosis of Campylobacter pylori infection.
The accuracy of various staining techniques for immersion microscopy, of five media for culture and five urease test modalities for the detection of Campylobacter pylori infection is reported. It was found that 2% urea unbuffered gel preparation is the most accurate urease test (sensitivity: 89%, specificity: 98%) but a minimal amount of 10,000 CFU/ml is necessary to observe positivity and the sensitivity of urease tests drops to 52% in patients under antimicrobial treatment. For histological diagnosis, modified Giemsa staining was shown to be slightly superior to H&E. The most valuable diagnosis technique is culture when the biopsy specimen is transported and processed under appropriate conditions. A 94% sensitivity rate was observed with 'BCC agar', a new medium containing brain heart infusion, activated charcoal and horse serum.
Deltenre
M
M
Department of Gastroenterology, Microbiology and Pathology, University Hospital Brugmann ULB-VUB, Brussels, Belgium.
Glupczynski
Y
Y
De Prez
C
C
Nyst
J F
JF
Burette
A
A
Labbé
M
M
Jonas
C
C
DeKoster
E
E
D001431:Bacteriological Techniques; D002167:Campylobacter / Q000201:enzymology; D002169:Campylobacter Infections / Q000175:diagnosis*; D004796:Clinical Enzyme Tests; D006652:Histological Techniques; D006801:Humans; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D014510:Urease / Q000378:metabolism
D014510:Urease