Stimulation of the Na+, K+ pump and the (Na+, K+, Cl-) cotransport system by endothelin-1 in cultured vascular smooth muscle cells: involvement of cyclo-oxygenase products.
The effect of endothelin (ET-1) on vascular smooth muscle cell Na+ and K+ regulation was studied in the A10 cultured cell line. In contrast with other vasoconstrictors, ET-1 was able to strongly stimulate both the Na+, K+ pump and the (Na+, K+, Cl-) cotransport system at concentrations higher than 10(-10) M. This did not appear to reflect a direct interaction with the transport proteins because ET-1 was without effect on the same transport systems in human red cells. Indomethacin, at concentrations of 10(-5) M, was able to counteract the pump and cotransport stimulation by ET-1. These results suggest that the potent vasoconstrictive action of ET-1 induces a negative feedback mechanism via a cyclo-oxygenase product, which enhances the ability of vascular smooth muscle cells to regulate Na+ and K+ contents.
Garay
R
R
INSERM U7, Hôpital Necker, Paris, France.
Rosati
C
C
Braquet
M
M
Chabrier
P E
PE
Braquet
P
P
D001693:Biological Transport, Active; D002118:Calcium / Q000097:blood; D002478:Cells, Cultured; D002712:Chlorides / Q000378:metabolism*; D016861:Cyclooxygenase Inhibitors; D016232:Endothelins; D004912:Erythrocytes / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D010455:Peptides / Q000494:pharmacology*; D011188:Potassium / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*
D002712:Chlorides; D016861:Cyclooxygenase Inhibitors; D016232:Endothelins; D010455:Peptides; D012964:Sodium; D011188:Potassium; D002118:Calcium; D007213:Indomethacin