Immunoelectron microscopic study of distribution of T cell subsets in oral lichen planus.
Monoclonal anti-CD4, anti-CD8, and anti-CD18 antibodies were applied in avidin-biotin-peroxidase complex staining using a pre-embedding immunoelectron microscopy technique. Although most of the local T cells in situ were of CD4+ subtype, local CD8+ cells generally had a lower nucleus/cytoplasm ratio and contained more cell organelles than CD4+ cells. This suggests a local activation of CD8+ subpopulation, rather than activation of the numerically predominant CD4+ cells. Topographical analysis disclosed that all lymphocytes, regardless of location, were CD18+ and that most of the CD8+ cells were located subbasally and intraepithelially, whereas CD4+ cells often occurred in small clusters deeper down in the subepithelial lymphocyte-rich band. Furthermore, CD8+ cells were often in close contact with macrophages, whereas CD4+ cells were in some instances in direct contact with plasma cells. This indicates that CD4+ cells may be involved in T cell-dependent B cell-mediated immunoglobulin synthesis, whereas CD8+ cytotoxic lymphocytes and tissue macrophages may be involved in the local pathogenetic process leading to basement membrane alterations.
Jungell
P
P
Department of Oral and Maxillofacial Surgery, Helsinki University, Finland.
Konttinen
Y T
YT
Nortamo
P
P
Malmström
M
M
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D018821:CD18 Antigens; D015704:CD4 Antigens / Q000032:analysis; D002467:Cell Nucleus / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D006801:Humans; D007124:Immunoenzyme Techniques; D008010:Lichen Planus / Q000473:pathology*; D008854:Microscopy, Electron; D009059:Mouth Diseases / Q000473:pathology*; D016028:Receptors, Leukocyte-Adhesion / Q000032:analysis; D013194:Staining and Labeling; D013601:T-Lymphocytes / Q000276:immunology* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D018821:CD18 Antigens; D015704:CD4 Antigens; D016028:Receptors, Leukocyte-Adhesion