Sequencing and synthesis of pardaxin, a polypeptide from the Red Sea Moses sole with ionophore activity.
Pardaxin, an amphipathic polypeptide secreted by the Red Sea flatfish Pardachirus marmoratus whose sequence is NH2-G-F-F-A-L-I-P-K-I-I-S-S-P-L-F-K-T-L-L-S-A-V-G-S-A-L-S-S-S-G-G-Q-E, was synthesized by the solid-phase method. The structure was verified by sequencing. The synthetic polypeptide changed the resistance of lipid bilayers by forming pores. At 10(-7)-10(-8) M, the synthetic pardaxin increased the frequency of the spontaneous release of quanta of acetylcholine at the neuromuscular junction by up to 100-fold, resembling the native product. Synthetic pardaxin seems to be a suitable tool for investigating the molecular structures underlying channel selectivity.
Shai
Y
Y
Molecular, Cellular and Nutritional Endocrinology Branch, NIDDK, Bethesda, MD 20892.
Fox
J
J
Caratsch
C
C
Shih
Y L
YL
Edwards
C
C
Lazarovici
P
P
D000109:Acetylcholine / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004553:Electric Conductivity; D005397:Fish Venoms / Q000138:chemical synthesis / Q000494:pharmacology; D005412:Flatfishes / Q000378:metabolism*; D007473:Ion Channels / Q000378:metabolism; D007476:Ionophores / Q000494:pharmacology*; D008051:Lipid Bilayers / Q000378:metabolism; D008969:Molecular Sequence Data; D009469:Neuromuscular Junction / Q000187:drug effects / Q000378:metabolism; D011487:Protein Conformation
D005397:Fish Venoms; D007473:Ion Channels; D007476:Ionophores; D008051:Lipid Bilayers; C017603:pardaxin; D000109:Acetylcholine