title: Glial hyaluronate-binding protein in Wallerian degeneration of dog spinal cord.
abstract: Wallerian degeneration of spinal cord dorsal columns was produced in three dogs by unilateral extradural dorsal rhizotomy at the lower thoracic level. The spinal cord was studied 1 month, 2 months, and 3 months after surgery. Transverse cryostat sections at the site rhizotomy and at the mid-thoracic level were stained by indirect immunofluorescence with antibodies to the glial fibrillary acidic protein (GFAP) and to the glial hyaluronate-binding protein (GHAP). GHAP immunoreactivity was almost unchanged in the degenerated dorsal column 1 month after rhizotomy. After 2 and 3 months, staining with GHAP antibodies was markedly decreased in the gliosed dorsal column at the site of rhizotomy, but it still persisted at the mid-thoracic level. It is concluded that GHAP persists for long periods of time in dorsal columns undergoing Wallerian degeneration, a finding consistent with its putative role as a nonpermissive substrate preventing nerve regeneration in CNS white matter.
authors: Spinal Cord Injury, Research Laboratory, Veterans Administration Medical Center, Boston, Massachusetts 02132.
authors: A
authors: A
authors: Bignami
authors: H
authors: H
authors: Mansour
authors: D
authors: D
authors: Dahl
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D004285:Dogs; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D018960:Hyaluronan Receptors; D007150:Immunohistochemistry; D009410:Nerve Degeneration / Q000502:physiology*; D009457:Neuroglia / Q000378:metabolism* / Q000502:physiology; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D014855:Wallerian Degeneration / Q000502:physiology*
chemical_list: D002352:Carrier Proteins; D005904:Glial Fibrillary Acidic Protein; D018960:Hyaluronan Receptors