title: Detection of turnip crinkle virus on agarose gel electropherograms at the nanogram load level.
abstract: The previous conditions for the physical characterization of turnip crinkle virus (TCV) by quantitative agarose gel electrophoresis [1, 2] were limiting the method to the microgram load level and were therefore insufficiently sensitive to satisfy the need in many areas of virology for detection of viruses containing single-stranded RNA at the nanogram level. The present report remedies that defect by presenting a technique compatible with the nanogram load level of such viruses. The technique is based on a reduction of gel thickness and on the use of silver staining.
authors: Section on Macromolecular Analysis, National Institute of Child Health and Human Development, Bethesda, MD 20892.
authors: L
authors: L
authors: Orbán
authors: A
authors: A
authors: Chrambach
mesh_terms: D004587:Electrophoresis, Agar Gel / Q000295:instrumentation; D004996:Ethidium; D005404:Fixatives; D010942:Plant Viruses / Q000032:analysis*; D012834:Silver; D013194:Staining and Labeling
chemical_list: D005404:Fixatives; D012834:Silver; D004996:Ethidium