A distinct subset of human CD4+ cells with a limited alloreactive T cell receptor repertoire.
We describe a subset of CD4+/CD3+ human T lymphocytes that demonstrated a remarkably limited TCR repertoire responding to alloantigen stimulation. These cells have been characterized previously by their granular morphology and expression of CD11b but not CD28. Whereas multiple CD28+/CD4+ alloproliferative cloned cell lines generated by culture at limiting dilution immediately after isolation from peripheral blood each had a unique TCR-beta gene rearrangement, 19 of 21 CD11b+/CD4+ clones showed identical TCR-beta, and gamma gene rearrangements. In conventional MLR, the CD11b+/CD4+ cells responded poorly after stimulation with some HLA-class II Ag, and staining with a TCR Id-specific antibody and DNA blot hybridization suggested that the responding CD11b+/CD4+ cells typically contained predominant clonal populations. Clones of CD11b+/CD4+ cells with different TCR gene rearrangements showed closely similar patterns of responses when stimulated by a panel of allogeneic PBMC, but the response pattern did not correspond to that of any known HLA-class II Ag. These findings indicate that CD11b+/CD4+ cells have a limited alloproliferative repertoire characterized by predominant recognition of a limited number of undefined determinants that appear to be expressed in association with multiple distinct HLA-class II Ag. Our results suggest that CD11b+/CD4+ cells are selected for clonal reactivity by processes distinct from those for CD28+/CD4+ cells.
Morishita
Y
Y
Fred Hutchinson Cancer Research Center, University of Washington, Seattle 98104.
Sao
H
H
Hansen
J A
JA
Martin
P J
PJ
D000943:Antigens, Differentiation / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D018106:CD28 Antigens; D017252:CD3 Complex; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002999:Clone Cells; D000939:Epitopes; D015329:Gene Rearrangement, T-Lymphocyte; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D016177:Macrophage-1 Antigen; D008285:Major Histocompatibility Complex; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology*; D016028:Receptors, Leukocyte-Adhesion / Q000276:immunology
D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D018106:CD28 Antigens; D017252:CD3 Complex; D000939:Epitopes; D016177:Macrophage-1 Antigen; D011948:Receptors, Antigen, T-Cell; D016028:Receptors, Leukocyte-Adhesion