Reactivity of mouse antibodies against bromelain-treated mouse erythrocytes with thrombin-treated mouse platelets.
The reactivity of mouse antibodies against bromelain-treated mouse erythrocytes (BrMRBC) with mouse platelets before and after thrombin treatment was assessed by flow cytometry. Anti-BrMRBC antibodies could bind to thrombin-treated platelets, although normal platelets were also weakly reactive with the antibodies. The binding of anti-BrMRBC antibodies to platelets was confirmed by complement-dependent lysis. It is suggested that thrombin-activated platelets may be a real target for anti-BrMRBC antibodies.
Kawaguchi
S
S
Department of Microbiology and Immunology, Shimane Medical University, Japan.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001792:Blood Platelets / Q000187:drug effects / Q000276:immunology*; D001963:Bromelains; D000939:Epitopes / Q000032:analysis; D004912:Erythrocytes / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D013917:Thrombin
D000911:Antibodies, Monoclonal; D000939:Epitopes; D001963:Bromelains; D013917:Thrombin