Release of highly-sulphated glycosaminoglycans and histamine from human basophils.
A new assay for highly-sulphated glycosaminoglycans (GAGs) has been applied to the study of allergen (Dermatophagoides pteronyssinus)-induced release of GAG from human basophils. Highly-sulphated GAG, which was not heparin, was found only in basophil-containing cellular fractions of whole blood. Its release, like that of histamine, was Ca++-dependent. However, allergen-induced release of GAG from the basophils of atopic individuals exhibited a different time-course and dose-response from that of histamine, and may occur at least in part by a different mechanism.
Reilly
K M
KM
MRC/SNBTS Blood Components Assay Group, Edinburgh, UK.
Dawes
J
J
Yap
P L
PL
Barnetson
R S
RS
MacGregor
I R
IR
D000328:Adult; D000941:Antigens / Q000494:pharmacology; D001491:Basophils / Q000378:metabolism*; D001667:Binding, Competitive; D002118:Calcium / Q000502:physiology; D002809:Chondroitin Sulfates / Q000097:blood; D003871:Dermatan Sulfate / Q000378:metabolism; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006025:Glycosaminoglycans / Q000378:metabolism*; D006497:Heparan Sulfate / Q000378:metabolism; D006636:Histamine Release; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000378:metabolism; D008297:Male; D008722:Methods; D008875:Middle Aged; D013997:Time Factors
D000941:Antigens; D002118:Calcium; D002809:Chondroitin Sulfates; D003871:Dermatan Sulfate; D006025:Glycosaminoglycans; D006497:Heparan Sulfate