title: Alcian blue staining of proteoglycans in polyacrylamide gels using the "critical electrolyte concentration" approach.
abstract: A method is described here for Alcian blue staining of proteoglycans in polyacrylamide gels; this is illustrated using extracts obtained from bovine corneal stroma. Other available methods for visualization of proteoglycans can produce nonspecific staining and frequently a high background in the gel; with a "critical electrolyte concentration" approach, specific staining against a clear background can be obtained.
authors: Open University, Oxford Research Unit, United Kingdom.
authors: R S
authors: RS
authors: Wall
authors: T J
authors: TJ
authors: Gyi
mesh_terms: D000423:Alcian Blue; D004591:Electrophoresis, Polyacrylamide Gel; D011509:Proteoglycans / Q000302:isolation & purification*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D011509:Proteoglycans; D000423:Alcian Blue