title: Cloning of the cDNA from normal brain and brain of patients with Alzheimer's disease in the expression vector lambda GT 11.
abstract: 1. RNA was purified from postmortem human brains, and the poly A+ RNA was isolated by oligo dT cellulose. 2. Double stranded cDNA was synthesized using reverse transcriptase, RNAse H and DNA polymerase. 3. cDNA was cloned in the lambda GT 11 expression vector, and libraries containing between 1 and 2 millions clones were obtained. 92 to 98% of the plaques contained a recombinant phage. 4. Such libraries will allow the molecular characterization of cDNA and corresponding proteins which play a key role in brain functions and in particular which could be involved in the etiology of Alzheimer's dementia.
authors: Université Catholique de Louvain, Laboratoire de Neurochimie, Brussels, Belgium.
authors: J N
authors: JN
authors: Octave
authors: F
authors: F
authors: de Sauvage
authors: A F
authors: AF
authors: Macq
authors: J M
authors: JM
authors: Maloteaux
mesh_terms: D000544:Alzheimer Disease / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics*; D001921:Brain / Q000378:metabolism*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D005822:Genetic Vectors; D006801:Humans; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012016:Reference Values
chemical_list: D012313:RNA; D004247:DNA