[Use of group A-specific polysaccharide antigen conjugated with protein carrier for detection of specific anti-polysaccharide antibodies].
Group-A specific polysaccharide antigen has been obtained by extraction of streptococcal suspension using 4N NaNO2 and glacial acetic acid, conjugated with bovine serum albumin using CNBr. Testing of the preparation with a set of rabbit anti-sera by enzyme immunoassay (EIA) and passive hemagglutination reaction (PHR) revealed its antigenic specificity both in direct experiments and in N-acetyl-D-glucosamine absorption. Forty-seven sera of EIA-studied individuals and 35 sera of PHR-studied ones, all with group-specific polysaccharide antigen, were examined. Immune reactions showed the broad spectrum of antibody titres in the studied sera. Complete correlation of anti-polysaccharide antibodies detected by the two techniques was observed in the sera with high specific antibody titres. A possibility to use the obtained group-A specific polysaccharide antigen to detect anti-polysaccharide antibodies in the sera of streptococcal infection patients is discussed.
Burova
L A
LA
Gladilina
M M
MM
Semykina
L V
LV
Murashov
S V
SV
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial / Q000276:immunology*; D003470:Culture Media; D000939:Epitopes; D006386:Hemagglutination Tests / Q000379:methods; D006801:Humans; D066298:In Vitro Techniques; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D013292:Streptococcus agalactiae / Q000276:immunology*; D013297:Streptococcus pyogenes / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D003470:Culture Media; D000939:Epitopes; D011135:Polysaccharides, Bacterial; D012710:Serum Albumin, Bovine
Ispo'lzovanie gruppospetsificheskogo A-polisakharidnogo antigena, ko''niugirovannogo s belkovym nositelem, dlia vyiavleniia spetsificheskikh antipolisakharidnykh antitel.