Purification and molecular identification of two protein methylases I from calf brain. Myelin basic protein- and histone-specific enzyme.
Two different molecular species of protein methylases I (S-adenosylmethionine:protein-arginine N-methyltransferase, EC 2.1.1.23), one specific for myelin basic protein (MBP) and the other for histone, have been purified from calf brain to near homogeneity, as discerned by nondenaturing polyacrylamide gel electrophoresis. Although both methylases share some common properties, such as utilization of S-adenosyl-L-methionine as the methyl donor and methylation of protein-bound arginine residues, they are distinctly different from each other in molecular weight and in catalytic, as well as the immunological, properties. The MBP-specific protein methylase I (approximately 500 kDa) methylates MBP preferentially (Km = 2 X 10(-7) M) and histone to a much lesser extent (Km = 1 X 10(-4) M), while the histone-specific methylase I (approximately 275 kDa) methylates histone only. Both methylases exhibit two major subunit bands on sodium dodecyl sulfate-polyacrylamide gel electrophoresis: 100 and 72 kDa for the MBP-specific and 110 and 75 kDa for the histone-specific. At 0.5 mM p-chloromercuribenzoate, about 50% of the MBP-specific enzyme remained as active, while most of the histone-specific enzyme activity was lost. In 2 mM guanidine HCl, approximately 90% of the former enzyme activity remained while nearly complete inactivation of the latter enzyme was observed. The enzymes also exhibited quite different inactivation profiles toward high temperature (45-65 degrees C); MBP-enzyme was stable up to 50 degrees C and was rapidly inactivated at higher temperatures with an inflection point at about 57 degrees C. However, under the identical conditions, histone-enzyme was inactivated progressively and linearly in the same temperature range. Finally, Western immunoblot analysis of polyclonal antibodies directed against either enzyme exhibited no cross-reactivity with the other.
Ghosh
S K
SK
Fels Research Institute, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
Paik
W K
WK
Kim
S
S
D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D002729:Chloromercuribenzoates / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D019791:Guanidine; D006146:Guanidines / Q000494:pharmacology; D006657:Histones / Q000378:metabolism*; D006358:Hot Temperature; D005779:Immunodiffusion; D007527:Isoenzymes / Q000302:isolation & purification*; D008970:Molecular Weight; D004676:Myelin Basic Protein / Q000378:metabolism*; D011496:Protein Methyltransferases / Q000302:isolation & purification*; D011484:Protein-Arginine N-Methyltransferases / Q000302:isolation & purification*; D013379:Substrate Specificity
D002729:Chloromercuribenzoates; D006146:Guanidines; D006657:Histones; D007527:Isoenzymes; D004676:Myelin Basic Protein; D011496:Protein Methyltransferases; D011484:Protein-Arginine N-Methyltransferases; D019791:Guanidine