title: Bleomycin-kanamycin resistance as a marker of the presence of transposon Tn5 in clinical strains of Escherichia coli.
abstract: The aminoglycoside modifying enzyme aminoglycoside 3'-phosphotransferase II (APH(3')II) is encoded for on transposon Tn5 by the aphA gene, in the same operon as the ble gene determining bleomycin resistance. To document this linkage 82 kanamycin-resistant Escherichia coli strains of clinical origin were studied; all 18 isolates presenting bleomycin-kanamycin resistance were shown by an enzymatic assay to produce APH(3')II, and the presence of Tn5 was demonstrated by gene hybridization. Similarly, bleomycin-kanamycin resistance was shown to be linked to APH(3')II production in Salmonella spp. The epidemiology of strains with Tn5-encoded APH(3')II may thus be studied, at least in Escherichia coli, by a simple diffusion test using bleomycin and kanamycin discs.
authors: Servicio de Microbiología, Hospital Ramón y Cajal, C., Madrid, Spain.
authors: F
authors: F
authors: Baquero
authors: M A
authors: MA
authors: Saldaña
authors: J
authors: J
authors: Blazquez
authors: R G
authors: RG
authors: Palacios
authors: J M
authors: JM
authors: Aguiar
authors: J L
authors: JL
authors: Martinez
authors: M F
authors: MF
authors: Vicente
authors: C
authors: C
authors: Rubio
authors: R
authors: R
authors: Gómez-Lus
mesh_terms: D001761:Bleomycin / Q000494:pharmacology*; D004251:DNA Transposable Elements; D004352:Drug Resistance, Microbial; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D006801:Humans; D019868:Kanamycin Kinase; D007613:Kanamycin Resistance / Q000235:genetics*; D010770:Phosphotransferases / Q000096:biosynthesis / Q000235:genetics; D012475:Salmonella / Q000187:drug effects / Q000201:enzymology / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements; D001761:Bleomycin; D010770:Phosphotransferases; D019868:Kanamycin Kinase