title: Corneal nerves contain intra-axonal HSV-1 after virus reactivation by epinephrine iontophoresis.
abstract: Experimental ocular models of herpes simplex virus type 1 (HSV-1) reactivation have been used to monitor viral shedding in the tear film and the appearance of corneal epithelial lesions, but the temporal correlation between reactivation and the presence of viral particles in the corneal nerves has not been made. Two New Zealand white rabbits were inoculated with 20 microliters of HSV-1 McKrae strain (5.0 x 10(6) PFU/ml) in each eye. Beginning on postinfection day 82, ocular iontophoresis (0.8 mAmps for 8 min) of 0.01% epinephrine was done once a day for 3 consecutive days to induce reactivation. Ten limbal nerves from four corneas processed for transmission electron microscopy contained 883 unmyelinated and 40 myelinated axons. Seven nerves were positive for virus. Viral particles were found only in unmyelinated axons, and in low frequency (24/883). Virus was not found in Schwann cells, perineurium, or adjacent stroma nor were virus particles seen exiting axons. No enveloped virions were found. Axons from six nerves of four control corneas from rabbits with latent, but not reactivated, HSV-1 did not contain virus particles. Induction by corneal iontophoresis of epinephrine suggests that HSV-1 is translocated from the ganglion to the cornea through axonal transport mechanisms. For the first time, evidence of anterograde, intra-axonal transport of HSV-1 particles in response to epinephrine reactivation is demonstrated.
authors: Lions Eye Research Laboratories, Louisiana State University Medical Center School of Medicine, New Orleans.
authors: L
authors: L
authors: Rivera
authors: R W
authors: RW
authors: Beuerman
authors: J M
authors: JM
authors: Hill
mesh_terms: D000818:Animals; D001370:Axonal Transport; D001369:Axons / Q000382:microbiology; D003315:Cornea / Q000294:innervation / Q000382:microbiology*; D003320:Corneal Ulcer / Q000175:diagnosis; D004837:Epinephrine / Q000494:pharmacology*; D007478:Iontophoresis; D007635:Keratitis, Dendritic / Q000382:microbiology*; D009412:Nerve Fibers / Q000648:ultrastructure; D011817:Rabbits; D018139:Simplexvirus / Q000302:isolation & purification*; D014775:Virus Activation
chemical_list: D004837:Epinephrine