Methylation at the CpG doublet in equine adenovirus genome.
Viral DNA obtained from the equine adenovirus propagated in equine transitional cell carcinoma (ETCC) cells and in equine fetal dermis cells were compared by cleaving with isoschizomeric restriction enzymes, HpaII and MspI, and then electrophoresed in 1.4 per cent agarose gels. Differences between the HpaII and MspI cleavage patterns were evident in viral DNA obtained only from the equine adenovirus propagated in ETCC cells, suggesting site specific methylation at CpG sequences.
Harasawa
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R
Department of Veterinary Microbiology, Faculty of Agriculture, Miyazaki University, Japan.
Higashi
T
T
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D002478:Cells, Cultured; D003596:Cytosine / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis / Q000378:metabolism*; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific; D005814:Genes, Viral; D006147:Guanine / Q000378:metabolism; D006736:Horses / Q000382:microbiology; D008745:Methylation; D014407:Tumor Cells, Cultured
D004279:DNA, Viral; D006147:Guanine; D003596:Cytosine; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific