title: Co-existence and origin of peptidergic and adrenergic nerves in the guinea pig uterus. Retrograde tracing and immunocytochemistry, effects of chemical sympathectomy, capsaicin treatment and pregnancy.
abstract: The occurrence and distribution of peptidergic nerves in the guinea pig uterus was studied by means of immunocytochemistry using numerous neuropeptide anti-sera. Neuropeptide Y (NPY)-immunoreactive (IR) nerves were the most abundant, whereas substance P (SP)-, calcitonin gene-related peptide (CGRP)-, and neurokinin A (NKA)-IR nerves were less frequent, and peptide histidine isoleucine (PHI)-IR nerves were the most sparse. Chemical sympathectomy by means of 6-hydroxydopamine, and capsaicin treatment revealed the division of the peptidergic nerves into three separate populations: (1) NPY-IR nerves, which co-existed with adrenergic nerves, (2) SP-, CGRP- and NKA-IR nerves, which mutually co-existed, and (3) PHI-IR nerves. Parallel-running adrenergic/NPY-IR and SP-IR nerves could be found with very similar although not completely identical morphological appearance. Paracervical ganglia contained neurotensin- and dynorphin A-IR cells bodies in addition to cell bodies with immunoreactivities similar to those in prevertebral ganglia. Combined retrograde tracing with True blue and immunocytochemistry showed that the adrenergic and NPY-IR uterine nerves originate in paracervical and prevertebral ganglia. In the prevertebral ganglia the cellular origin was the same for adrenergic and NPY-IR nerves. In contrast, SP-, CGRP-, and NKA-IR nerves originated in dorsal root ganglia. At full-term pregnancy all the neuropeptide immunoreactivities had vanished, probably reflecting a fetus-induced general nerve degeneration.
authors: Department of Pathology, University of Lund, Sweden.
authors: P
authors: P
authors: Alm
authors: L M
authors: LM
authors: Lundberg
mesh_terms: D000320:Adrenergic Fibers / Q000033:anatomy & histology* / Q000378:metabolism; D000818:Animals; D015740:Calcitonin Gene-Related Peptide; D002211:Capsaicin; D002584:Cervix Uteri / Q000294:innervation; D005260:Female; D005455:Fluorescent Antibody Technique; D005727:Ganglia, Spinal / Q000276:immunology; D006168:Guinea Pigs; D006892:Hydroxydopamines; D007150:Immunohistochemistry; D015288:Neurokinin A / Q000378:metabolism; D009478:Neuropeptide Y / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism / Q000502:physiology*; D016627:Oxidopamine; D011247:Pregnancy; D013373:Substance P / Q000378:metabolism; D013552:Swine / Q000033:anatomy & histology*; D013563:Sympathectomy, Chemical; D014599:Uterus / Q000294:innervation* / Q000378:metabolism
chemical_list: D006892:Hydroxydopamines; D009478:Neuropeptide Y; D009479:Neuropeptides; D013373:Substance P; D015288:Neurokinin A; D016627:Oxidopamine; D015740:Calcitonin Gene-Related Peptide; D002211:Capsaicin