title: Methylation in the 5' region of the murine beta Fc gamma R gene regulates the expression of Fc gamma receptor II.
abstract: In order to identify possible mechanisms regulating the expression of Fc gamma RII, we have examined the methylation status of the beta Fc gamma R gene in a panel of Fc gamma RII (+) and (-) cells belonging to several different lineages. We used beta 1 cDNA probes, derived from beta Fc gamma R gene transcripts which encode murine Fc gamma RII molecules. We found that all CCGG sequences detected with these probes were methylated in the genomic DNA of the Fc gamma RII-(-) cells. By contrast, two CCGG sites were found to be selectively unmethylated in the DNA of all Fc gamma RII(+) cells tested. These sites could be assigned to the region of the 5' end of the beta Fc gamma R gene. Besides, the treatment of Fc gamma RII(-) thymoma cells BW5147 with 5-azacytidine induced a hypomethylation of the beta Fc gamma R gene concomitantly with the transcription of that gene as seen by Northern blotting and the expression of functional Fc gamma RII. Conversely, the DNA-methylating agent ethyl methanesulfonate completely reversed the phenotype of the 5-azacytidine-treated cells to that of the Fc gamma RII(-) BW5147 parent cells. In ethyl methanesulfonate-treated cells, the beta Fc gamma R gene was remethylated and the corresponding transcript was no more detectable. We conclude that the methylation of a specific 5' segment of the beta Fc gamma R gene regulates the expression of Fc gamma RII in murine T cells, B cells, mast cells, and macrophages, possibly by controlling the gene transcription.
authors: Laboratoire d'Immunologie Cellulaire el Clinique, Institut Curie, Paris, France.
authors: C
authors: C
authors: Bonnerot
authors: M
authors: M
authors: Daëron
authors: N
authors: N
authors: Varin
authors: S
authors: S
authors: Amigorena
authors: P M
authors: PM
authors: Hogarth
authors: J
authors: J
authors: Even
authors: W H
authors: WH
authors: Fridman
mesh_terms: D000818:Animals; D001374:Azacitidine / Q000494:pharmacology; D002460:Cell Line; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D007074:Immunoglobulin G / Q000235:genetics / Q000378:metabolism*; D008745:Methylation; D051379:Mice; D011961:Receptors, Fc / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D017452:Receptors, IgG; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism
chemical_list: D007074:Immunoglobulin G; D011961:Receptors, Fc; D017452:Receptors, IgG; D004247:DNA; D005020:Ethyl Methanesulfonate; D001374:Azacitidine