Interferons as gene activators: a cluster of six interferon-activatable genes is linked to the erythroid alpha-spectrin locus on murine chromosome 1.
Several interferon-activatable murine genes were mapped to murine chromosomes by hybridizing cDNA probes to Southern blots of genomic DNA samples from a panel of mouse-hamster somatic cell hybrid lines. The 12 gene is located on chromosome 12 and it specifies a 3.6-kb mRNA. The 204 gene (specifying a 2.5-kb mRNA), and three genes of the 203 gene family (hybridizing to five mRNAs of sizes between 2 and 4.5 kb), together with the 202 gene (specifying a 2-kb mRNA) are located on murine chromosome 1. By restriction fragment length polymorphism analysis of DNA samples prepared from a panel of recombinant inbred mouse lines (C57BL/6J D DBA/2J) and from 85 [C3H/HeJ-gld/gld x Mus spretus) F1 X C3H/HeJ-gld/gld] backcross mice we established a close linkage of the 202, 203, and 204 genes to the erythroid alpha-spectrin gene (Spna-1) on distal murine chromosome 1. Cosmids containing the 202, 203, and 204 genes were isolated from a library derived from AKR mouse DNA. Southern blot analysis of such cosmids revealed: (a) hybridization of a partial 203 cDNA to three genes of the 203 gene family; (b) cross-hybridization of the 202 and 204 genes with one another and with a third gene (designated as 201 gene), and (c) a close linkage of genes of the 203 family with the 201, 202, and 204 genes. These results indicate the existence of a cluster of at least six closely linked, interferon-activatable genes on distal murine chromosome 1 in the vicinity of the Spna-1 locus and also of the Minor lymphocyte stimulating locus (Mlsa).
Opdenakker
G
G
Department of Molecular Biophysics, Yale University, New Haven, Connecticut 06511.
Snoddy
J
J
Choubey
D
D
Toniato
E
E
Pravtcheva
D D
DD
Seldin
M F
MF
Ruddle
F H
FH
Lengyel
P
P
D000818:Animals; D015139:Blotting, Southern; D002874:Chromosome Mapping; D015342:DNA Probes; D005786:Gene Expression Regulation / Q000187:drug effects*; D008040:Genetic Linkage; D007372:Interferons / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D013049:Spectrin / Q000235:genetics*
D015342:DNA Probes; D013049:Spectrin; D007372:Interferons