Interleukin-4 induces secretion of CSF for granulocytes and CSF for macrophages by peripheral blood monocytes.
T cells are known to interact cooperatively with monocytes to produce Colony-Stimulating Factors (CSF), although T cell-mediated signals leading to CSF secretion by monocytes are not completely understood. We have made use of Northern blot hybridization and specific bioassays to study the effects of the T cell product interleukin-4 (IL-4) on monocyte CSF expression. The results suggest a previously unrecognized role of IL-4 as a CSF inducer since exposure of monocytes to IL-4 resulted in accumulation of transcripts for granulocyte-CSF (G-CSF) and macrophage-CSF (M-CSF). Consequently, IL-4-activated monocytes released factors in their culture supernatants biologically and antigenically indistinguishable from G- and M-CSF.
Wieser
M
M
Department of Hematology, Johannes Gutenberg University, Mainz, FRG.
Bonifer
R
R
Oster
W
W
Lindemann
A
A
Mertelsmann
R
R
Herrmann
F
F
D000818:Animals; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000096:biosynthesis* / Q000302:isolation & purification; D016179:Granulocyte Colony-Stimulating Factor; D006098:Granulocytes; D006801:Humans; D015847:Interleukin-4; D007378:Interleukins / Q000494:pharmacology*; D016173:Macrophage Colony-Stimulating Factor; D008264:Macrophages; D051379:Mice; D008809:Mice, Inbred C3H; D009000:Monocytes / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D014158:Transcription, Genetic
D003115:Colony-Stimulating Factors; D007378:Interleukins; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D015847:Interleukin-4; D016173:Macrophage Colony-Stimulating Factor