Human immunodeficiency virus type 1 challenge of chimpanzees immunized with recombinant envelope glycoprotein gp120.
The major envelope glycoprotein, gp120, of human immunodeficiency virus type 1 (HIV-1) was purified from a Chinese hamster ovary cell line transfected with a truncated form of the HIV-1 env gene. The recombinant glycoprotein (rgp120) was formulated with aluminum hydroxide adjuvant and was used to immunize chimpanzees. The recombinant preparation was effective in eliciting cellular and humoral immunity as well as immunologic memory. Anti-rgp 120 antibodies reacted with authentic viral gp120 in immunological blot assays and were able to neutralize HIV-1 infectivity in vitro. Sera from the rgp120-immunized animals were able to neutralize HIV-1 pseudotypes of vesicular stomatitis virus prepared from the IIIB isolate, from which the gene encoding rgp120 was derived, as well as two heterologous isolates, ARV-2 and RF. The immune response elicited against the rgp120 was not effective in preventing viral infection after intravenous challenge with HIV-1. The implications of these results on HIV-1 vaccine development are discussed.
Berman
P W
PW
Department of Molecular Biology, Genentech, Inc., South San Francisco, CA 94080.
Groopman
J E
JE
Gregory
T
T
Clapham
P R
PR
Weiss
R A
RA
Ferriani
R
R
Riddle
L
L
Shimasaki
C
C
Lucas
C
C
Lasky
L A
LA
D000163:Acquired Immunodeficiency Syndrome / Q000517:prevention & control; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000096:biosynthesis; D000941:Antigens / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D006678:HIV / Q000276:immunology* / Q000502:physiology; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; D007106:Immune Sera / Q000276:immunology; D007111:Immunity, Cellular; D007114:Immunization; D009500:Neutralization Tests; D002679:Pan troglodytes / Q000276:immunology*; D012191:Retroviridae Proteins / Q000235:genetics / Q000276:immunology*; D018894:Reverse Transcriptase Inhibitors; D013601:T-Lymphocytes / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*; D010948:Viral Plaque Assay
D000914:Antibodies, Viral; D000941:Antigens; D000956:Antigens, Viral; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; D007106:Immune Sera; D012191:Retroviridae Proteins; D018894:Reverse Transcriptase Inhibitors; D014614:Vaccines, Synthetic