Cloning of GMP-140, a granule membrane protein of platelets and endothelium: sequence similarity to proteins involved in cell adhesion and inflammation.
GMP-140 is an integral membrane glycoprotein found in secretory granules of platelets and endothelial cells. After cellular activation, it is rapidly redistributed to the plasma membrane. The cDNA-derived primary structure of GMP-140 predicts a cysteine-rich protein with multiple domains, including a "lectin" region, an "EGF" domain, nine tandem consensus repeats related to those in complement-binding proteins, a transmembrane domain, and a short cytoplasmic tail. Some cDNAs also predict a soluble protein with a deleted transmembrane segment. The domain organization of GMP-140 is similar to that of ELAM-1, a cytokine-inducible endothelial cell receptor that binds neutrophils. This similarity suggests that GMP-140 belongs to a new family of inducible receptors with related structure and function on vascular cells.
Johnston
G I
GI
St. Francis Medical Research Institute, University of Oklahoma Health Sciences Center, Oklahoma City.
Cook
R G
RG
McEver
R P
RP
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002448:Cell Adhesion; D003001:Cloning, Molecular; D003594:Cytoplasmic Granules / Q000032:analysis* / Q000648:ultrastructure; D004247:DNA / Q000032:analysis / Q000302:isolation & purification; D004730:Endothelium, Vascular / Q000032:analysis* / Q000166:cytology / Q000648:ultrastructure; D006706:Homeostasis; D006801:Humans; D007249:Inflammation / Q000378:metabolism / Q000503:physiopathology*; D007425:Intracellular Membranes / Q000032:analysis; D008533:Megakaryocytes / Q000032:analysis* / Q000166:cytology / Q000648:ultrastructure; D008565:Membrane Proteins / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D014176:Protein Biosynthesis
D008565:Membrane Proteins; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D004247:DNA