Isoelectric focusing on cellulose acetate membranes: an up-date on materials and methods.
The method of isoelectric focusing proteins on cellulose acetate membranes has been improved by the introduction of: (i) plastic-backed membranes, (ii) a commercially available Mini-IEF cell and (iii) sensitive colloidal gold staining. The improved methodology is described with applications for clinical laboratories.
Ambler
J
J
Clinical Chemistry Department, University Hospital, Queen's Medical Centre, Nottingham, England.
D000665:Ampholyte Mixtures; D002482:Cellulose / Q000031:analogs & derivatives*; D006046:Gold; D006453:Hemoglobinopathies / Q000097:blood; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing / Q000379:methods*; D008567:Membranes, Artificial; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid; D010641:Phenotype; D013194:Staining and Labeling; D000515:alpha 1-Antitrypsin / Q000235:genetics
D000665:Ampholyte Mixtures; D008567:Membranes, Artificial; D000515:alpha 1-Antitrypsin; C005062:acetylcellulose; D006046:Gold; D002482:Cellulose