[Expression of Drosophila retrotransposon in yeast].
The recombinant plasmids pPTX and pPGX were constructed, containing the sequences of yeast Ty retrotransposon and Drosophila element mdg4, correspondingly. Transformation of yeast by these plasmids lead to induction of reverse transcriptase activity associated with virus-like particles, containing only the sequences of Ty. The data obtained show that mdg4 is capable of expression in yeast and the products of its expression are used to form the yeast virus-like particles. The system described may be used to study the expression of different retrotransposons from various cells in yeast.
Reznik
N L
NL
Cheperegin
S E
SE
Gorelova
T V
TV
Arman
I P
IP
Shuppe
N G
NG
D000818:Animals; D004251:DNA Transposable Elements; D004330:Drosophila / Q000235:genetics*; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D010957:Plasmids; D012194:RNA-Directed DNA Polymerase / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004251:DNA Transposable Elements; D012194:RNA-Directed DNA Polymerase
Ekspressiia retrotranspozona drozofily v drozhzhakh.