title: Human T cell recognition of polymorphic epitopes from malaria circumsporozoite protein.
abstract: Lymphocytes obtained from forty individuals living in a malaria endemic area of West Africa were tested for in vitro proliferative responses to peptides representing variant regions of the immunodominant T cell domain of the circumsporozoite protein (amino acids 326 to 345, referred to as Th2R, and 361 to 380, referred to as Th3R) from three distinct strains of Plasmodium falciparum. A total of 83% of the individuals responded to at least one of the six peptides tested, confirming that these epitopes are immunodominant. A much greater number of individuals than expected by chance (32% of the responders to Th2R and 27% of the responders to Th3R) reacted to all three of the variant peptides for that epitope, indicating interdependency of the T cell responses, suggestive of cross-reactivity. Nevertheless, some subjects' T cells were clearly able to distinguish each variant peptide from the others. Using EBV transformed B cells, lymphocytes from 10 of the individuals were HLA typed. In this small group, HLA DRw13 was associated with a positive response to any of the peptides, whereas there was a negative association between DQw3 and response to any of the peptides. These results, although limited by the small sample size, suggest that recognition of T epitopes may be Ir gene linked. Our findings suggest that it may be possible to broaden the immunogenicity of an anti-sporozoite malaria vaccine.
authors: Laboratory of Parasitic Diseases, National Institutes of Allergy and Infectious Disease, Bethesda, MD 20892.
authors: A S
authors: AS
authors: de Groot
authors: A H
authors: AH
authors: Johnson
authors: W L
authors: WL
authors: Maloy
authors: I A
authors: IA
authors: Quakyi
authors: E M
authors: EM
authors: Riley
authors: A
authors: A
authors: Menon
authors: S M
authors: SM
authors: Banks
authors: J A
authors: JA
authors: Berzofsky
authors: M F
authors: MF
authors: Good
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D000954:Antigens, Surface / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D006650:Histocompatibility Testing; D006801:Humans; D008213:Lymphocyte Activation; D008288:Malaria / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000235:genetics / Q000276:immunology; D011110:Polymorphism, Genetic; D015800:Protozoan Proteins; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000954:Antigens, Surface; D000939:Epitopes; D015800:Protozoan Proteins; C041595:circumsporozoite protein, Protozoan