Constitutive expression of a 2',5'-oligoadenylate synthetase cDNA results in increased antiviral activity and growth suppression.
The interferon (IFN)-induced enzyme 2',5'-oligoadenylate (2-5A) synthetase has been implicated in the development of antiviral activity in human and animal cells. However, its role in IFN-mediated growth inhibition remains unclear. To elucidate the function of 2-5A synthetase, we have stably introduced a human 2-5A synthetase cDNA into a human glioblastoma cell line (T98G). Constitutive expression of the cDNA in these cells is associated with increased levels of resistance to infection by encephalomyocarditis virus. One transfected subclone, which expresses elevated levels of 2-5A synthetase enzyme activity, also shows a reduced rate of cellular proliferation.
Rysiecki
G
G
Division of Infectious Diseases, Hospital for Sick Children, Toronto, Ontario, Canada.
Gewert
D R
DR
Williams
B R
BR
D015088:2',5'-Oligoadenylate Synthetase / Q000235:genetics* / Q000502:physiology; D000998:Antiviral Agents / Q000502:physiology; D002455:Cell Division; D002999:Clone Cells / Q000201:enzymology; D004247:DNA / Q000235:genetics*; D004686:Encephalomyocarditis virus; D005786:Gene Expression Regulation; D006801:Humans; D007372:Interferons / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000201:enzymology
D000998:Antiviral Agents; D012333:RNA, Messenger; D004247:DNA; D007372:Interferons; D015088:2',5'-Oligoadenylate Synthetase