Pertussis toxin activates protein kinase C and a tyrosine protein kinase in the human T cell line Jurkat.
Pertussis toxin activates T lymphocytes by a mechanism that is independent of its ADP-ribosylation activity. The toxin stimulates increases in diacylglycerol and intracellular calcium apparently by interacting with a cell surface receptor. Consistent with the production of these second messengers we have found that pertussis toxin activates protein kinase C in the Jurkat cell line. The toxin was also found to activate a tyrosine protein kinase in these cells in a manner similar to that observed with phytohemagglutinin. These results provide evidence that the mechanism of activation of T cells by pertussis toxin involves stimulating the activity of protein kinase C and a tyrosine protein kinase.
Thom
R E
RE
Department of Pharmacology, University of Rochester, School of Medicine and Dentistry, NY 14642.
Casnellie
J E
JE
D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D004075:Diglycerides / Q000378:metabolism; D004789:Enzyme Activation; D006801:Humans; D015151:Immunoblotting; D008970:Molecular Weight; D037342:Pertussis Toxin; D010766:Phosphorylation; D019000:Phosphotyrosine; D010835:Phytohemagglutinins / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D013601:T-Lymphocytes / Q000201:enzymology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D004075:Diglycerides; D010835:Phytohemagglutinins; D010566:Virulence Factors, Bordetella; D019000:Phosphotyrosine; D014443:Tyrosine; D037342:Pertussis Toxin; D011505:Protein-Tyrosine Kinases; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium