Protective effect of misoprostol, a synthetic prostaglandin E1 analog, on experimental pancreatitis induced by pancreatic duct ligation in rat.
This study was performed to assess the effects of misoprostol (M), a synthetic prostaglandin E1 analog, on experimental pancreatitis in rat. Pancreatitis was induced by ligation of the main pancreatic duct of 3-month-old male Wistar rats. Pancreatic lesions were observed at 6, 12, 24, 48, and 96 h after pancreatic duct ligation (PDL). A time of 48 h was chosen to evaluate M treatment. M was injected intraperitoneally (500 micrograms/kg every 4 h) between time 0 and 48 h after PDL. Stereological analysis was performed on light and electron microscopy. Total pancreatic amylase and chymotrypsin concentrations were determined. Four groups of five rats were studied: sham operated (SO), M without PDL (PG), duct ligation without M (DL), and duct ligation with M (DLPG). Edema, dedifferentiation of pancreatic acinar cells, and heterogeneous distribution of zymogen granule diameters observed after PDL were significantly decreased by M in the DLPG group. Enzyme concentrations were also decreased by M in the DLPG group. Enzyme concentrations were also decreased by M both in normal (PG) and duct ligated rats (DL). M has protective effects against pancreatic lesions induced by PDL. In this model, the protective effect of M may be due to a blockade of the autodigestive secretions of the pancreatic acinar cells.
Buscail
L
L
INSERM U151, CHU Rangueil, Toulouse, France.
Sénégas-Balas
F
F
Balas
D
D
Bouisson
M
M
Bertrand
C
C
Ribet
A
A
D000527:Alprostadil / Q000494:pharmacology / Q000627:therapeutic use; D000681:Amylases / Q000378:metabolism; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002918:Chymotrypsin / Q000378:metabolism; D006651:Histocytochemistry; D008026:Ligation; D008297:Male; D016595:Misoprostol; D010179:Pancreas / Q000473:pathology / Q000601:surgery; D010183:Pancreatic Ducts; D010195:Pancreatitis / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D011459:Prostaglandins E, Synthetic / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D011919:Rats, Inbred Strains
D000897:Anti-Ulcer Agents; D011459:Prostaglandins E, Synthetic; D016595:Misoprostol; D000681:Amylases; D002918:Chymotrypsin; D000527:Alprostadil