Effects of long-term storage on the concentrations of the unconjugated acidic metabolites of the trace amines, indoleamines and catecholamines.
The concentrations of urinary phenylacetic, m- and p-hydroxyphenylacetic, mandelic (MA), p-hydroxymandelic, indoleacetic, homovanillic, vanillylmandelic, 5-hydroxyindoleacetic and 3,4-dihydroxyphenylacetic (DOPAC) acids and 3-methoxy-4-hydroxyphenylethyleneglycol (MHPG) and the concentrations of the above plasma acids (except MA, MHPG and DOPAC) were determined by gas chromatography-mass spectrometry with selected-ion monitoring at the time of receipt of the samples and after three, six and nine months storage at -18 degrees C. The samples were not treated in any way before storage. The concentrations of most of the urinary metabolites declined significantly (analysis of variance) over the nine-month storage period, whereas most of the plasma metabolites did not.
Davis
B A
BA
Neuropsychiatric Research Unit, University of Saskatchewan, Saskatoon, Canada.
D000588:Amines / Q000378:metabolism* / Q000652:urine; D002395:Catecholamines / Q000097:blood / Q000378:metabolism* / Q000652:urine; D006719:Homovanillic Acid / Q000097:blood / Q000378:metabolism / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006897:Hydroxyindoleacetic Acid / Q000097:blood / Q000378:metabolism / Q000652:urine; D008027:Light; D008734:Methoxyhydroxyphenylglycol / Q000097:blood / Q000378:metabolism / Q000652:urine; D012701:Serotonin / Q000097:blood / Q000378:metabolism* / Q000652:urine; D013696:Temperature; D013997:Time Factors; D014642:Vanilmandelic Acid / Q000097:blood / Q000378:metabolism / Q000652:urine
D000588:Amines; D002395:Catecholamines; D012701:Serotonin; D008734:Methoxyhydroxyphenylglycol; D006897:Hydroxyindoleacetic Acid; D014642:Vanilmandelic Acid; D006719:Homovanillic Acid