Localization of the human type 5, tartrate-resistant acid phosphatase gene by in situ hybridization.
We report the localization of the gene for the human type 5, tartrate-resistant, iron-containing acid phosphatase isoenzyme (HGM designation ACP5) to chromosome 15 (15q22-q26) using the technique of in situ hybridization to metaphase chromosomes. We have localized this gene using peripheral blood chromosomes obtained from both a normal male and an individual carrying an unbalanced translocation involving chromosome 15 [45,XY,-15,-18,+der(18)-t(15;18)(q13;p11)]. In addition, we have demonstrated the utility of employing a standard fluorescent staining technique (distamycin/DAPI) to an emulsion-coated, Wright-stained, and destained chromosome preparation.
Allen
B S
BS
Department of Biochemistry and Molecular Biology, University of Florida, Gainesville 32610.
Ketcham
C M
CM
Roberts
R M
RM
Nick
H S
HS
Ostrer
H
H
D000135:Acid Phosphatase / Q000235:genetics*; D002874:Chromosome Mapping; D002884:Chromosomes, Human, Pair 15; D002887:Chromosomes, Human, Pair 18; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D013194:Staining and Labeling; D014178:Translocation, Genetic
D015342:DNA Probes; D007527:Isoenzymes; D004247:DNA; D000135:Acid Phosphatase