title: Taurine and beta-alanine act on both GABA and glycine receptors in Xenopus oocyte injected with mouse brain messenger RNA.
abstract: The responding pathway (process from agonist binding to channel opening) of taurine and beta-alanine was investigated in Xenopus oocytes injected with mouse brain poly(A)+ RNA. Responses to gamma-aminobutyric acid (GABA), glycine, taurine and beta-alanine were induced in oocytes injected with poly(A)+ RNA extracted from 3 regions, cerebrum, cerebellum and brainstem of the mouse brain. From comparison, responses to these 4 inhibitory amino acids in each regional poly(A)+ RNA-injected oocytes were categorized into at least 3 groups: (1) GABA, (2) glycine, and (3) taurine and beta-alanine. No cross-desensitization was observed between GABA response and glycine response, but taurine and beta-alanine responses cross-desensitized both the GABA and glycine responses. Taurine and beta-alanine responses were partially inhibited by the GABA antagonist, bicuculline, and also by the glycine antagonist, strychnine. The results suggest that the taurine or the beta-alanine response in the brain is caused through both the GABA receptor and the glycine receptor.
authors: Department of Physiology, School of Dental Medicine, Tsurumi University, Yokohama, Japan.
authors: T
authors: T
authors: Horikoshi
authors: A
authors: A
authors: Asanuma
authors: K
authors: K
authors: Yanagisawa
authors: K
authors: K
authors: Anzai
authors: S
authors: S
authors: Goto
mesh_terms: D000409:Alanine / Q000494:pharmacology*; D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D001921:Brain / Q000378:metabolism*; D005260:Female; D005998:Glycine / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D008845:Microinjections; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D009928:Organ Specificity; D011061:Poly A / Q000008:administration & dosage / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000008:administration & dosage / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics*; D011963:Receptors, GABA-A / Q000187:drug effects*; D018009:Receptors, Glycine; D017981:Receptors, Neurotransmitter / Q000187:drug effects*; D013331:Strychnine / Q000494:pharmacology; D013654:Taurine / Q000494:pharmacology*; D014982:Xenopus laevis; D015091:beta-Alanine / Q000494:pharmacology*; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
chemical_list: D012333:RNA, Messenger; D011963:Receptors, GABA-A; D018009:Receptors, Glycine; D017981:Receptors, Neurotransmitter; D015091:beta-Alanine; D013654:Taurine; D011061:Poly A; D005680:gamma-Aminobutyric Acid; D012313:RNA; D013331:Strychnine; D000409:Alanine; D005998:Glycine; D001640:Bicuculline