Archaebacterial histone-like proteins. Purification and characterization of helix stabilizing DNA binding proteins from the acidothermophile Sulfolobus acidocaldarius.
Four DNA binding histone-like proteins have been purified from the nucleoid of the acidothermophilic archaebacterium Sulfolobus acidocaldarius to homogeneity employing DNA-cellulose chromatography and carboxymethylcellulose chromatography. The molecular weights of these proteins are in the range 8,000-12,500. Immunoblotting results suggest that a few antigenic determinants are common among these proteins which could not be detected by immunodiffusion. Spectroscopic properties of the proteins have been studied. The amino acid compositions of these proteins show both similarities and differences with histones and prokaryotic histone-like proteins. All of the four proteins bind native and denatured DNAs and single stranded RNA with differing affinities. Three of the proteins, denoted by HSNP (helix stabilizing nucleoid protein)-A, HSNP-C, and HSNP-C', show physiologically significant, strong, and synergistic effects in stabilizing duplex DNA against thermal denaturation with Tm increases in the range of 15-30 +/- degrees C.
Reddy
T R
TR
School Of Life Sciences, University of Hyderabad, India.
Suryanarayana
T
T
D000596:Amino Acids / Q000032:analysis; D001105:Archaea / Q000032:analysis*; D001419:Bacteria / Q000032:analysis*; D002467:Cell Nucleus / Q000032:analysis; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002845:Chromatography; D002942:Circular Dichroism; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D006657:Histones / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D006358:Hot Temperature; D015151:Immunoblotting; D005779:Immunodiffusion; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation / Q000187:drug effects; D012313:RNA / Q000378:metabolism; D013053:Spectrophotometry
D000596:Amino Acids; D004268:DNA-Binding Proteins; D006657:Histones; D012313:RNA; D004247:DNA