[Sequential analysis of the human corneal endothelium in vitro].
A corneal endothelium staining technique is described which can be performed in a few minutes. The first step of this in vitro staining is atraumatic for the endothelium. The cell density and the number of dead cells are thus determined. The second step is a staining of nuclear and intercellular spaces with a fixation by ethanol. Which then permits an histological analysis. This method of the endothelium, as described, thus allows for the control of corneal button either before grafts or after preservation, as well as the analysis of corneal pathologies.
Delbosc
B
B
Clinique Ophtalmologique, C.H.U. Jean Minjoz, Besançon.
Piquot
X
X
Montard
M
M
D000880:Anthraquinones; D002452:Cell Count / Q000379:methods; D002470:Cell Survival; D016039:Corneal Transplantation; D004728:Endothelium, Corneal / Q000033:anatomy & histology* / Q000473:pathology; D005110:Extracellular Space; D006801:Humans; D009926:Organ Preservation; D013194:Staining and Labeling / Q000379:methods; D014343:Trypan Blue
D000880:Anthraquinones; C004468:Alizarin Red S; D014343:Trypan Blue
Analyse séquentielle de l'endothélium cornéen humain in vitro.