title: A modified Brown and Hopps stain for identification of gram-positive and gram-negative microorganisms in glycol methacrylate-embedded tissues.
abstract: Plastic embedding is a recently developed technique that has been shown to be superior to conventional paraffin embedding in the histopathologic identification of microorganisms in tissue specimens. This report describes a modification of the Brown and Hopps stain for the differentiation of gram-positive and gram-negative microorganisms in 3-micron-thick plastic sections. This technique is easily performed and has a rapid turnaround time. This staining method permits a more precise histopathologic diagnosis of infectious agents than is possible in paraffin-embedded tissues.
authors: Department of Pathology and Laboratory Medicine, Hahnemann University School of Medicine, Philadelphia.
authors: D A
authors: DA
authors: Schwartz
authors: R
authors: R
authors: Munger
authors: B
authors: B
authors: Lazzaro
authors: G
authors: G
authors: Lumb
mesh_terms: D000179:Acrylates; D000818:Animals; D005658:Fungi / Q000145:classification / Q000302:isolation & purification; D006090:Gram-Negative Bacteria / Q000145:classification / Q000302:isolation & purification*; D006094:Gram-Positive Bacteria / Q000145:classification / Q000302:isolation & purification*; D006652:Histological Techniques; D006801:Humans; D008689:Methacrylates; D051381:Rats; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D000179:Acrylates; D008689:Methacrylates; C005044:hydroxyethyl methacrylate