Characterization of two monoclonal antibodies raised against human testicular cells.
Human testicular cells isolated from biopsy tissue were used for generation of monoclonal antibodies. Two hybridoma supernatants C3 and D4 were selected according to their reaction with sperm-precursor cells (immature sperms) in an enzyme-linked immunosorbent assay (ELISA). C3 reacted with testicular but no other tissue. D4 did not reveal any pattern of testicular staining in spite of its similarity to C3 in binding to sperm-precursor cells in ELISA and microcytotoxicity test.
Kurpisz
M
M
Polish Academy of Scineces, Department of Histology and Experimental Embryology, Medical School, Poznan.
Mapp
P
P
Lukaszyk
A
A
Ogilvie
J
J
Festenstein
H
H
Sachs
J
J
D000328:Adult; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D002469:Cell Separation; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D008297:Male; D012708:Sertoli Cells / Q000276:immunology*; D013094:Spermatozoa / Q000276:immunology*; D013194:Staining and Labeling; D013737:Testis / Q000166:cytology / Q000276:immunology*
D000911:Antibodies, Monoclonal