title: Three-dimensional location of ribosomal protein BL2 from Bacillus stearothermophilus, a key component of the peptidyl transferase center.
abstract: Protein BL2 from Bacillus stearothermophilus has been localized by immunoelectron microscopy on the interface side of the 50 S subunit, beneath the angle formed between the central protuberance and the L1 protuberance. The immuno-electron microscopic data suggest that the interface region of the 50 S particle is not as flat as most of the proposed three-dimensional models suggest, but instead there is a significant concavity. Since several studies demonstrated that BL2 is implicated in peptidyl transferase activity or at least located close to the peptidyl transferase center, the location of protein BL2 also provides information as to the location of this important functional domain.
authors: Max-Plank-Institut für Molekulare Genetik, Abteilung Wittmann, Berlin, Dahlem, Germany.
authors: W
authors: W
authors: Hackl
authors: M
authors: M
authors: Stöffler-Meilicke
authors: G
authors: G
authors: Stöffler
mesh_terms: D000217:Acyltransferases / Q000032:analysis*; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D000939:Epitopes / Q000276:immunology; D001411:Geobacillus stearothermophilus / Q000032:analysis*; D007106:Immune Sera / Q000276:immunology; D007118:Immunoassay; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G / Q000378:metabolism; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D010458:Peptidyl Transferases / Q000032:analysis*; D012269:Ribosomal Proteins / Q000032:analysis* / Q000276:immunology
chemical_list: D000939:Epitopes; D007106:Immune Sera; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D046911:Macromolecular Substances; D012269:Ribosomal Proteins; C029466:ribosomal protein L2; D000217:Acyltransferases; D010458:Peptidyl Transferases