Effects of adenosine on neutrophil polarization induced by N-formyl-methionyl-leucyl-phenylalanine, sodium propionate and colchicine.
Polarization of human neutrophils (a characteristic bipolar shape change) can be induced by the chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine (FMLP); sodium propionate, which causes a rapid acidification of the cytosol; or colchicine, which disrupts microtubules. We have previously reported that adenosine, endogenously produced in human neutrophil suspensions, inhibits FMLP-induced polarization. We report here that endogenously produced adenosine also inhibits sodium propionate-induced polarization but has no effect on colchicine-induced polarization. These results suggest that neutrophil polarization may be a multistep process inducible by compounds that trigger different biochemical events.
Iannone
M A
MA
Wellcome Research Laboratories, Research Triangle Park, NC 27709.
Wolberg
G
G
D000241:Adenosine / Q000494:pharmacology*; D003078:Colchicine / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D008859:Microscopy, Polarization; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects* / Q000648:ultrastructure; D011422:Propionates / Q000494:pharmacology*; D013194:Staining and Labeling
D011422:Propionates; D009240:N-Formylmethionine Leucyl-Phenylalanine; C514135:sodium propionate; C029658:propionic acid; D000241:Adenosine; D003078:Colchicine