Induction of cytochrome P-450IA1 gene expression in human breast tumour cell lines.
The induction of cytochrome P-450IA1 by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) was studied in eight human breast tumour cell lines. The cells were treated with various concentrations of TCDD for 24 h, and total RNA was isolated. The level of P-450IA1 RNA induced by 1 nM TCDD followed the order: MCF-7 greater than T47-D greater than ZR-75-1 greater than 3909 greater than 3522. AL-1, BT-20 and CAMA-1 did not respond to TCDD at the concentrations used. Northern blot analysis revealed 2 bands at 2.7 and 2.0 Kb, respectively, with the larger band being 6-fold more intense. The ratio was not changed by the TCDD treatment. TCDD induction did not change the benzo[a]pyrene-7,8-diol (BP-7,8-diol) metabolite profile compared with control cells, when cells were incubated with [3H]BP-7,8-diol for 24 h following the treatment with TCDD. These results demonstrate that different breast tumour cell lines vary greatly with respect to the basal expression levels of P-450IA1 RNA and its inducibility by TCDD. Furthermore, TCDD treatment does not change the relative distribution of BP-7,8-diol metabolites.
Pasanen
M
M
Laboratory of Environmental Carcinogenesis, Fibiger Institute, Danish Cancer Society, Copenhagen Denmark.
Stacey
S
S
Lykkesfeldt
A
A
Briand
P
P
Hines
R
R
Autrup
H
H
D001943:Breast Neoplasms / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004101:Dihydroxydihydrobenzopyrenes / Q000378:metabolism; D004147:Dioxins / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D009693:Nucleic Acid Hybridization; D000072317:Polychlorinated Dibenzodioxins / Q000494:pharmacology*; D012313:RNA / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D004101:Dihydroxydihydrobenzopyrenes; D004147:Dioxins; D000072317:Polychlorinated Dibenzodioxins; C017228:benzo(a)pyrene 7,8-dihydrodiol; D012313:RNA; D003577:Cytochrome P-450 Enzyme System