title: Nippostrongylus brasiliensis: radioresistant IgE antibody-forming cells in infected rats.
abstract: In Nippostrongylus brasiliensis-infected rats, anti-N. brasiliensis IgE antibody production was observed at 20 weeks postinfection, long after the worms, as a source of antigen, had been expelled. The persistent IgE production was not abrogated after whole body irradiation (800 R) administered at 12 or 20 weeks, suggesting the participation of radioresistant IgE-forming cells. Help of T cells and recruitment of B memory cells in the irradiated rats seems to be ruled out by the findings that the irradiation completely inhibited the initiation of anti-N. brasiliensis IgE production in rats shortly after the infection with N. brasiliensis or after primary and secondary immunization with N. brasiliensis-antigen. Moreover, clearance of anti-N. brasiliensis IgE antibody from circulation did not seem to be crucially affected by the irradiation. The radioresistant cells forming anti-N. brasiliensis IgE were most productive in mesenteric lymph nodes as compared to other lymph nodes. The recognition of antigens fractionated by chromatography on Sephadex G-200 was the same for IgE-forming cells from rats 12 weeks after infection as for those from 3 weeks after infection. Based on these results, one of the mechanisms of persistent elevation of IgE antibody in the host infected with helminth parasites might be explained by the participation of radioresistant IgE-forming cells.
authors: Department of Parasitology, Jikei University School of Medicine, Tokyo, Japan.
authors: N
authors: N
authors: Watanabe
authors: A
authors: A
authors: Kobayashi
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis*; D000921:Antibody-Producing Cells / Q000276:immunology / Q000528:radiation effects*; D000947:Antigens, Helminth / Q000276:immunology; D002850:Chromatography, Gel; D000939:Epitopes / Q000032:analysis; D007073:Immunoglobulin E / Q000096:biosynthesis*; D009349:Nematode Infections / Q000276:immunology*; D009559:Nippostrongylus / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D000939:Epitopes; D007073:Immunoglobulin E