[Phorbol myristate acetate promotes entry of calcium in hypophysial cells GH3 B6 via potential dependent calcium channels].
10 GH3/B6 cells were patched-clamped using a pipette containing NMG as internal cation, 2 mM ATP and 100 microM leupeptin. Whole-cell calcium or barium currents were recorded prior and after PMA (10(-8) or 10(-7) M). PMA increased the inward calcium current at potential levels close to threshold in 8 cells; 7 cells only exhibited an increase in transitory calcium current at potential levels close to threshold; in one cell, both transitory and conventional calcium currents were increased. 2 cells did not respond to PMA.
Taupignon
A I
AI
Laboratoire de Neurophysiologie-Unité Associée au CNRS 1200, Université de Bordeaux II.
Dufy
B
B
D000818:Animals; D001464:Barium / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D004594:Electrophysiology; D007473:Ion Channels / Q000187:drug effects* / Q000378:metabolism; D007976:Leupeptins / Q000494:pharmacology; D010902:Pituitary Gland / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D007473:Ion Channels; D007976:Leupeptins; D001464:Barium; C032854:leupeptin; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium
Le phorbol myristate acétate favorise l'entrée de calcium dans les cellules hypophysaires GH3 B6 par les canaux calciques potentiel-dépendants.