[3H-thymidine and 3H-uridine incorporation into the heart cells of the freshwater crayfish Astacus astacus and the swimming crab Portunus holsatus].
DNA and RNA syntheses in the heart cells of two decapod species were investigated with the aid of electron microscopic autoradiography. Isotopes were injected in the cavity of adult animals 4 hours before fixation. 3H-thymidine labeling was found in several satellite cell nuclei and in some particular epicardial cell nuclei. None of myonuclei was labeled. 3H-uridine incorporated in all the nuclei of muscle fibers. Satellite cells were labeled with 3H-uridine very slightly, if at all. Such a peculiarity of biosynthetic processes in the decapod heart satellite cell suggests their myoblastic nature similar to that of satellite cells of somatic muscles. The active 3H-thymidine uptake by the heart satellite cells of adult animals may be accounted for by the permanent growth of the decapods through their whole life span.
Martynova
M G
MG
D000818:Animals; D003400:Astacoidea / Q000378:metabolism*; D001345:Autoradiography; D003386:Brachyura / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis; D008854:Microscopy, Electron; D009206:Myocardium / Q000378:metabolism* / Q000648:ultrastructure; D012313:RNA / Q000096:biosynthesis; D013936:Thymidine / Q000378:metabolism*; D014316:Tritium; D014529:Uridine / Q000378:metabolism*
D014316:Tritium; D012313:RNA; D004247:DNA; D013936:Thymidine; D014529:Uridine
Vkliuchenie 3H-timidina i 3H-uridina v kletki serdtsa rechnogo raka Astacus astacus i kraba-plavuntsa Portunus holsatus.