A novel interleukin stimulating free radical production by granulocytes.
Polymorphonuclear granulocytes (PMN) are potent producers of free oxygen-derived radicals. Since other granulocyte functions are affected by interleukins, we investigated whether free-radical production can be initiated by a similar mediator. For estimation of free radical production, SOD-inhibitable lucigenin-dependent chemiluminescence and SOD-inhibitable cytochrome C reduction were used. As a source of interleukins, serum-free 24 h culture supernatants of human mononuclear cells (MNC) stimulated with bacterial lipopolysaccharide were prepared. Addition of such supernatants to PMN caused stimulation of sod-inhibitable chemiluminescence and superoxide production. Studies with separated MNC showed that monocytes were the cellular source of the activity. Biochemically, this activity of the supernatants was due to a heat-labile glycoprotein with a MW of approx. 60 KDa. This mediator, termed granulocyte chemiluminescence inducer (GCI), appears to be distinct from interleukin 1 (alpha and beta) and interferon (alpha and gamma). In conclusion we describe a novel monokine, granulocyte chemiluminescence inducer (GCI), which initiates granulocyte free radical production. This interaction of monocytes and granulocytes may also in vivo constitute a new and potent pathway leading to stimulation of free oxygen production by granulocytes.
Maly
F E
FE
Institute of Clinical Immunology, Inselspital, Bern, Switzerland.
Kapp
A
A
De Weck
A L
AL
D000911:Antibodies, Monoclonal; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D005362:Ficoll; D005609:Free Radicals; D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007372:Interferons / Q000032:analysis; D007375:Interleukin-1 / Q000302:isolation & purification / Q000494:pharmacology; D007378:Interleukins / Q000302:isolation & purification / Q000494:pharmacology*; D008163:Luminescent Measurements; D010084:Oxidation-Reduction; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism
D000911:Antibodies, Monoclonal; D003470:Culture Media; D005609:Free Radicals; D007375:Interleukin-1; D007378:Interleukins; D011994:Recombinant Proteins; D013481:Superoxides; D005362:Ficoll; D007372:Interferons; D006861:Hydrogen Peroxide