Characterizing T-cell epitopes in vaccine candidates.
T-cell clones can be used to test in vitro whether synthetic peptides associate with major histocompatibility complex (MHC) antigens - a necessary condition for the peptides to be immunogenic. Generally, the T-cell clones have been obtained from immune donors, but J.R.L. Pink and F. Sinigaglia argue here that non-immune, human leukocyte antigen (HLA)-typed donors are a useful source of clones and antigen-presenting cells that can be used to assay systematically peptide-MHC associations.
Pink
J R
JR
Sinigaglia
F
F
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology*; D006168:Guinea Pigs; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D007114:Immunization; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology; D011485:Protein Binding; D013601:T-Lymphocytes / Q000276:immunology*; D014612:Vaccines / Q000276:immunology*
D000939:Epitopes; D006680:HLA Antigens; D010446:Peptide Fragments; D014612:Vaccines