Aspartame and the rat brain monoaminergic system.
A high dose of aspartame (APM) was administered to rats to study possible effects on brain monoaminergic systems. APM and its metabolite phenylalanine (Phe) were given orally at doses of 1000 and 500 mg/kg, respectively. Significant increases were seen in brain Phe and tyrosine (Tyr) levels. Two different approaches were used to study monoaminergic systems: whole tissue measurements by HPLC-ED and in vivo voltammetry in freely moving rats. Dopamine, serotonin and their metabolites were taken as indexes of neuronal activity. In spite of the high dose used, no modification was found in monoamines or their metabolites in striatum, hippocampus and nucleus accumbens.
Perego
C
C
Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
De Simoni
M G
MG
Fodritto
F
F
Raimondi
L
L
Diomede
L
L
Salmona
M
M
Algeri
S
S
Garattini
S
S
D015102:3,4-Dihydroxyphenylacetic Acid / Q000032:analysis; D000818:Animals; D001218:Aspartame / Q000008:administration & dosage* / Q000494:pharmacology; D015306:Biogenic Monoamines / Q000378:metabolism*; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003342:Corpus Striatum / Q000032:analysis; D004151:Dipeptides / Q000008:administration & dosage*; D004298:Dopamine / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D004567:Electrodes, Implanted; D006624:Hippocampus / Q000032:analysis; D006897:Hydroxyindoleacetic Acid / Q000032:analysis; D009714:Nucleus Accumbens / Q000032:analysis; D010649:Phenylalanine / Q000378:metabolism; D010949:Plasma / Q000032:analysis; D051381:Rats; D012701:Serotonin / Q000032:analysis; D014443:Tyrosine / Q000032:analysis
D015306:Biogenic Monoamines; D004151:Dipeptides; D015102:3,4-Dihydroxyphenylacetic Acid; D012701:Serotonin; D014443:Tyrosine; D010649:Phenylalanine; D006897:Hydroxyindoleacetic Acid; D004298:Dopamine; D001218:Aspartame