Two-dimensional gel electrophoresis to separate large RNA molecules such as messenger RNAs.
This paper describes a two-dimensional gel electrophoresis method for separating large RNA molecules such as messenger RNAs. RNAs were at first separated on a polyacrylamide plus agarose composite gel and subjected to a second dimension electrophoresis on a polyacrylamide gel containing urea. This method is illustrated by analyses of poly(A)+ yeast RNAs. About 80 discrete spots were detected on the gel, when RNAs from 1000 to 3500 nucleotides in size were examined.
Ikemura
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DNA Research Center, National Institute of Genetics, Shizuoka-ken, Japan.
D004587:Electrophoresis, Agar Gel / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D011061:Poly A / Q000302:isolation & purification; D012313:RNA / Q000302:isolation & purification; D012331:RNA, Fungal / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000235:genetics
D012331:RNA, Fungal; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA