Effects of nicardipine on blood pressure and platelet cytosolic free calcium concentration in SHR.
Intracellular free calcium concentration was measured by the fluorescent calcium indicator quin2 method in platelets of spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats, and the effects of nicardipine on platelet cytosolic free calcium concentration were investigated. Cytosolic free calcium concentration was significantly increased in platelets of 4-, 11-, and 14-week-old SHR compared with age-matched WKY rats. Platelet cytosolic free calcium concentration in 14-week-old male SHR was significantly decreased 1 h after the administration of nicardipine (5 mg/kg p.o.), while that in WKY rats remained unchanged. These results suggest that transmembrane Ca2+ influx is genetically increased in SHR.
Baba
A
A
Department of Medicine, Wakayama Medical College, Japan.
Fukuda
K
K
Kuchii
M
M
Hano
T
T
Nishio
I
I
Masuyama
Y
Y
D000284:Administration, Oral; D000634:Aminoquinolines; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D001794:Blood Pressure / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism*; D005456:Fluorescent Dyes; D008297:Male; D009529:Nicardipine / Q000494:pharmacology*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
D000634:Aminoquinolines; D005456:Fluorescent Dyes; C035738:Quin2-acetoxymethyl ester; D009529:Nicardipine; D002118:Calcium