Quality control in immunohistochemistry. Report of a workshop sponsored by the Biological Stain Commission.
Although immunohistochemical methods are increasingly applied in diagnostic histopathology, there has been little standardization or quality control of immunoreagents; and published reports have not standardized Material and Methods for meaningful comparisons of results among clinicians. The Biological Stain Commission-sponsored workshop was convened to address the following issues: a manufacturers' testing program for probity of commercial antibodies, development of a manual for performance criteria and quality control assurance procedures, standardization of package inserts, standardization of information provided in the Materials and Methods sections of publications, establishment of a reagent and procedure clearing house, study of the effects of different fixation regimes on tissue antigens, and investigation of the environmental conditions needed for antigen-antibody interaction. The recommendations of the ad hoc committee and their implications for the future are discussed.
Elias
J M
JM
University of New York, Stony Brook School of Medicine.
Gown
A M
AM
Nakamura
R M
RM
Wilbur
D C
DC
Herman
G E
GE
Jaffe
E S
ES
Battifora
H
H
Brigati
D J
DJ
D000936:Antigen-Antibody Complex / Q000302:isolation & purification; D005404:Fixatives / Q000592:standards; D007150:Immunohistochemistry / Q000592:standards*; D007255:Information Services / Q000592:standards; D009937:Organizational Objectives; D011786:Quality Control; D011933:Reagent Kits, Diagnostic / Q000592:standards; D012015:Reference Standards; D013194:Staining and Labeling / Q000592:standards
D000936:Antigen-Antibody Complex; D005404:Fixatives; D011933:Reagent Kits, Diagnostic