Assay of serum hyaluronic acid in clinical application.
A sandwich-binding protein assay to determine the concentration of hyaluronic acid (HA) in body fluids has been developed. In this method, a hyaluronic acid binding protein (HABP) was adsorbed to the surface of a solid phase, and HA bound to HABP on the solid phase was detected by biotin-conjugated HABP. The method could assay HA levels within 6 hours using precoated microwells with HABP. HA could be determined in the range of 2-500 micrograms/l by this method using 50 microliters of serum. Within-run precision (CV) was 5.2-10.2%. The specificity of HABP to HA was confirmed by the elimination of the reaction with treatment by hyaluronidase digestion. Serum HA levels (median; range) of patients with rheumatoid arthritis (34; 2-187 micrograms/l) were shown to be higher than those with osteoarthritis (1; 1-21 micrograms/ml) and healthy controls (2; 1-8 micrograms/ml). No correlation between levels of HA and rheumatoid factor was found. HA was demonstrated to be a potential diagnostic marker for rheumatoid arthritis, and this HABP assay could be useful for determination of HA in clinical laboratory tests.
Chichibu
K
K
Chugai Pharmaceutical Co. Ltd., Tokyo, Japan.
Matsuura
T
T
Shichijo
S
S
Yokoyama
M M
MM
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001172:Arthritis, Rheumatoid / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D001710:Biotin; D002352:Carrier Proteins / Q000302:isolation & purification; D006801:Humans; D018960:Hyaluronan Receptors; D006820:Hyaluronic Acid / Q000097:blood*; D006821:Hyaluronoglucosaminidase; D006868:Hydrolysis; D008875:Middle Aged; D010003:Osteoarthritis / Q000097:blood*; D013223:Statistics as Topic
D015415:Biomarkers; D002352:Carrier Proteins; D018960:Hyaluronan Receptors; D001710:Biotin; D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase