Galanin inhibits noradrenaline-induced accumulation of cyclic AMP in the rat cerebral cortex.
The effect of galanin on noradrenaline (NA)-induced accumulation of cyclic AMP was investigated in slices of rat cerebral cortex. NA (10(-4)M) increased cyclic AMP levels during a 20-min observation period. Galanin (3 X 10(-7)M) significantly inhibited this response at all time points examined, although it did not change the basal levels of cyclic AMP. Galanin (10(-8)-3 X 10(-6)M) inhibited the cyclic AMP response to NA (10(-4)M) in a dose-dependent manner, with an IC50 of approximately 5.6 X 10(-8)M and a maximum inhibition of 59%. These results suggest that galanin, devoid of any detectable effects by itself, modulates the cyclic AMP response to NA in the rat cerebral cortex.
Nishibori
M
M
Department of Pharmacology, Okayama University Medical School, Japan.
Oishi
R
R
Itoh
Y
Y
Saeki
K
K
D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D000242:Cyclic AMP / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D019004:Galanin; D007700:Kinetics; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology*; D010455:Peptides / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D010455:Peptides; D019004:Galanin; D000242:Cyclic AMP; D009638:Norepinephrine