[Radioautographic demonstration of the intracellular and extracellular bactericidal action of the neutrophils].
The authors described a method of quantitative radioautographic analysis of RNA synthesis level in the bacteria. The method allows to define by inhibition of RNA synthesis a bactericidal action of neutrophils during phagocytosis. The essence of a method consists in the introduction for a short period of a label and development of radioautographs at semithin section by paraphenylenediamine which form a small and numerous silver grains above bacteria which retained an ability to RNA synthesis. Preparations obtained in this way is studied in a light optical microscope using a double illumination: from below through a condenser and from above through an objective.
Pal'tsyn
A A
AA
Badikova
A K
AK
Chervonskaia
N V
NV
Ustinova
T S
TS
Uchaneishvili
E K
EK
D000818:Animals; D001345:Autoradiography / Q000379:methods; D001419:Bacteria / Q000378:metabolism; D001770:Blood Bactericidal Activity; D006801:Humans; D008854:Microscopy, Electron / Q000379:methods; D009504:Neutrophils / Q000276:immunology* / Q000648:ultrastructure; D010587:Phagocytosis; D010655:Phenylenediamines; D012329:RNA, Bacterial / Q000096:biosynthesis
D010655:Phenylenediamines; D012329:RNA, Bacterial; C029728:4-phenylenediamine
Radioavtograficheskoe vyiavlenie intratselliuliarnogo i ékstratselliuliarnogo bakteritsidnogo deĭstviia neĭtrofilov.