Inter-alpha-trypsin inhibitor is a complex of three different protein species.
We found that, in contrast to earlier reports, inter-alpha-trypsin inhibitor of human serum is not a single-chain glycoprotein, but a protein complex. Three different protein components which are encoded on three independent mRNAs could be demonstrated by sequencing of cloned cDNA as well as of peptides obtained from purified inter-alpha-trypsin inhibitor. Accordingly, after fractionation of plasma by ammonium sulfate precipitation, ion-exchange chromatography, zinc-chelate chromatography, and SDS-polyacrylamide gel electrophoresis three proteins can be detected in Western blots using antibodies directed against inter-alpha-trypsin inhibitor.
Gebhard
W
W
Abteilung für Klinische Chemie, Chirurgischen Klinik Innenstadt, Universität München.
Leysath
G
G
Schreitmüller
T
T
D000510:Alpha-Globulins / Q000032:analysis*; D000645:Ammonium Sulfate; D055598:Chemical Phenomena; D002621:Chemistry; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007118:Immunoassay; D012967:Sodium Dodecyl Sulfate
D000510:Alpha-Globulins; D012967:Sodium Dodecyl Sulfate; C011877:inter-alpha-inhibitor; D000645:Ammonium Sulfate