Assay of pyrethroid-hydrolysing esterases using (1,R)-cis-3-(2,2-dibromovinyl)-2,2-dimethyl cyclopropane carboxylates as substrates.
1. p-Nitrophenyl-, alpha-naphthyl-, and beta-naphthyl-(1,R)-cis-3-(2,2-dibromovinyl)2,2-dimethyl cyclopropane carboxylates are proposed as pyrethroid-like substrates for the detection of pyrethroid esterases (PyEs). 2. The substrates have been used in a spectrophotometric assay of PyEs and in a staining method to locate PyEs on polyacrylamide electrophoresis.
Wallace
G
G
CIPEIN (CITEFA-CONICET), Buenos Aires, Argentina.
Casabé
N
N
Wood
E
E
Zerba
E
E
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D004950:Esterases / Q000032:analysis*; D006793:Houseflies / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D011722:Pyrethrins / Q000378:metabolism*; D013053:Spectrophotometry / Q000379:methods; D013194:Staining and Labeling; D013379:Substrate Specificity; D013552:Swine; D014224:Triatoma / Q000201:enzymology
D011722:Pyrethrins; D004950:Esterases