Generation of thyroglobulin-specific T-cell clones derived from F1 Fisher rats.
We established T-cell clones from 'poor-responder' Fisher rats specific for thyroglobulin (Tg) with a view to examining Tg presentation by cloned Fisher rat thyroid (FRTL) cells. From the screening of 60 T-cell clones, three high-responding Tg-specific clones (B21.01, B21.04 and B21.05) were isolated from the lymph nodes of F1 generation (Fisher x 'high-responder' Buffalo) female rats immunized with murine Tg in complete Freund's adjuvant (CFA). Three T-cell clones expressed a W3/25+, OX-8- phenotype and responded specifically to murine and rat Tg in T-cell proliferation assays with Fisher rat antigen-presenting cells and secreted IL-2 as measured using a murine cytotoxic T-lymphocyte line (CTLL-2). Both B21.04 and B21.05 T-cell clones were capable of providing helper T-cell function for rat Tg antibody production in syngeneic reconstitution cultures in vitro. In contrast, clone B21.01 inhibited Tg antibody secretion. These data demonstrate that 'poor-responder' Fisher rats are capable of mounting significant T-cell responses to Tg in an F1 generation. Such Tg-specific T-cell clones will allow us to analyse their interaction with cloned Fisher rat thyroid cells and determine the role, if any, of thyroid cell antigen self-presentation to the immune system.
Hirose
W
W
Department of Medicine, Mount Sinai School of Medicine, New York, New York.
Davies
T F
TF
D000818:Animals; D000917:Antibody Formation; D000938:Antigen-Presenting Cells / Q000276:immunology; D000941:Antigens / Q000276:immunology; D002478:Cells, Cultured; D002999:Clone Cells; D000939:Epitopes; D007376:Interleukin-2 / Q000096:biosynthesis; D008938:Mitosis; D010641:Phenotype; D051381:Rats; D011919:Rats, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D013954:Thyroglobulin / Q000276:immunology*
D000941:Antigens; D000939:Epitopes; D007376:Interleukin-2; D013954:Thyroglobulin