title: The effect of age of cattle on the in vitro production of interferon by peripheral blood mononuclear cells.
abstract: Peripheral blood mononuclear cells (PBMC) from normal cattle of different ages and from specific pathogen-free (SPF) calves, 2 to 4 weeks old, were cultured with bovine herpes virus type 1 (BHV1), parainfluenza-3 virus (PI3) and phytohaemagglutinin (PHA). The interferon (IFN) produced was characterized by acid stability and neutralizing antisera to recombinant bovine interferons. The virus preparations were presented either live or inactivated and as cell-bound virus or free virions. PBMC from cattle of all ages produced IFN-alpha when stimulated with live BHV1 and PI3 viruses. IFN-alpha was also produced with inactivated BHV1, even in cell cultures from SPF calves. However, inactivated PI3 virus failed to induce IFN in PBMC cultures from normal cattle, but approximately half of the animals, mostly calves, produced IFN-gamma spontaneously in 48 h cultures in the absence of added antigen. PHA induced IFN-gamma at an optimal concentration of 20 micrograms per ml after 3 days in culture. An age-related maturation of the IFN response was observed as PBMC from calves less than 2 weeks old produced little or no IFN when induced with either PHA or inactivated BHV1, although some IFN-alpha was produced in cultures containing live virus. Both adherent and non-adherent cells from adults and calves over 2 weeks old produced IFN on induction with inactivated BHV1 but only the non-adherent cell population produced IFN spontaneously or in response to inactivated PI3.
authors: Department of Clinical Veterinary Medicine, University of Cambridge, U.K.
authors: J
authors: J
authors: Townsend
authors: W P
authors: WP
authors: Duffus
authors: D J
authors: DJ
authors: Williams
mesh_terms: D000375:Aging / Q000097:blood*; D000818:Animals; D001773:Blood Cells / Q000378:metabolism; D002417:Cattle / Q000097:blood*; D002448:Cell Adhesion; D002478:Cells, Cultured; D007372:Interferons / Q000096:biosynthesis*; D009000:Monocytes / Q000378:metabolism*; D009500:Neutralization Tests; D010835:Phytohemagglutinins / Q000494:pharmacology; D014777:Virus Diseases / Q000097:blood / Q000662:veterinary; D018406:Virus Physiological Phenomena
chemical_list: D010835:Phytohemagglutinins; D007372:Interferons