Antibodies against the non-muscle isoform of the endoplasmic reticulum Ca2(+)-transport ATPase.
We report here the production of a polyclonal antiserum which specifically recognizes an epitope confined to the ultimate 12-residue-long C-terminus of an alternatively spliced transcript of gene 2 encoding the sarcoplasmic reticulum Ca2+ pump in slow skeletal and cardiac muscle. This alternatively spliced transcript was shown to be mainly represented in non-muscle tissues. These antibodies have enabled us to show the presence of the unique C-terminus of this type of Ca2+ pump, as predicted from the cDNA sequence, in the endoplasmic reticulum of vascular and gastric smooth muscle, liver and kidney.
Wuytack
F
F
Laboratorium voor Fysiologie, Katholieke Universiteit Leuven, Belgium.
Eggermont
J A
JA
Raeymaekers
L
L
Plessers
L
L
Casteels
R
R
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D001011:Aorta / Q000201:enzymology; D000252:Calcium-Transporting ATPases / Q000235:genetics* / Q000276:immunology; D002417:Cattle; D002462:Cell Membrane / Q000201:enzymology; D004721:Endoplasmic Reticulum / Q000201:enzymology*; D000939:Epitopes / Q000032:analysis*; D005796:Genes; D007527:Isoenzymes / Q000235:genetics* / Q000276:immunology; D008861:Microsomes / Q000201:enzymology; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000201:enzymology*; D009131:Muscle, Smooth, Vascular / Q000201:enzymology; D009132:Muscles / Q000201:enzymology*; D009842:Oligopeptides / Q000138:chemical synthesis; D010446:Peptide Fragments / Q000032:analysis; D012326:RNA Splicing; D011817:Rabbits / Q000276:immunology; D013270:Stomach / Q000201:enzymology; D013552:Swine; D014158:Transcription, Genetic
D000906:Antibodies; D000939:Epitopes; D007527:Isoenzymes; D009842:Oligopeptides; D010446:Peptide Fragments; D000252:Calcium-Transporting ATPases