[A staining method of PIVKA-II by an indirect immunoenzyme technic].
The localization of PIVKA-II in liver tissue was studied by the immuno-staining method using monoclonal antibody. The cell lines derived from hepatoblastoma (huH-6), hepatocellular carcinoma (PLC/PFR/5), and normal liver (Chang) were used as materials for staining. We succeeded to stain PIVKA-II, when the materials were fixed in a formalin solution and frozen. In patients with hepatocellular carcinoma, PIVKA-II was stained in the cytoplasm of both hepatoma and non-hepatoma cells.
Tanimoto
K
K
Onji
M
M
Nadano
S
S
Kumon
I
I
Michitaka
K
K
Horiike
N
N
Ohta
Y
Y
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis; D006801:Humans; D007124:Immunoenzyme Techniques; D008099:Liver / Q000032:analysis*; D008113:Liver Neoplasms / Q000175:diagnosis; D011498:Protein Precursors / Q000032:analysis*; D011516:Prothrombin / Q000032:analysis*; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D011498:Protein Precursors; C008208:acarboxyprothrombin; D011516:Prothrombin