Mapping of monoclonal antibodies to human factor IX.
We used recombinant DNA techniques to map a panel of six monoclonal antibodies (MoAbs) to regions of the human factor IX molecule. A-2 maps to 17 amino acids at the amino terminus of the heavy chain of IXa; 2D5, an inhibitor of clotting, is defined to 36 amino acids of the first EGF-like domain of human factor IX. A-4, A-5, C10D, and FXC008 all map to a region of the heavy chain containing amino acids 180 through 310, suggesting an immunodominant site. FXC008 has been reported to interfere with binding of factor IXa to factor VIII:Ca.
Frazier
D
D
Department of Biology, University of North Carolina, Chapel Hill 27599-3280.
Smith
K J
KJ
Cheung
W F
WF
Ware
J
J
Lin
S W
SW
Thompson
A R
AR
Reisner
H
H
Bajaj
S P
SP
Stafford
D W
DW
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000915:Antibody Affinity; D001667:Binding, Competitive; D003001:Cloning, Molecular; D000939:Epitopes / Q000302:isolation & purification; D005164:Factor IX / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping; D010449:Peptide Mapping / Q000379:methods
D000911:Antibodies, Monoclonal; D000939:Epitopes; D005164:Factor IX