A synthetic, partial pre-mRNA for ovalbumin primes its own complementary DNA with reverse transcriptase.
Synthetic, partial pre-mRNA for ovalbumin was found to prime its own cDNA with reverse transcriptase. Initiation of the cDNA occurred 36 bases upstream of the 3'-end of the RNA, probably as a result of intramolecular base pairing at this end. Inhibition of self-priming occurs following ligation of pCp or poly-adenylation at the 3'-terminus of the synthetic RNA. The secondary structure of 3'-end region of the template RNA strongly affected the ability of self-priming.
Oyama
F
F
Mitsubishi Kasei Institute of Life Sciences, Tokyo.
Kikuchi
R
R
Uchida
T
T
D001483:Base Sequence; D003565:Cytidine Diphosphate; D004247:DNA / Q000096:biosynthesis / Q000235:genetics*; D009690:Nucleic Acid Conformation; D010047:Ovalbumin / Q000235:genetics*; D010957:Plasmids; D011061:Poly A; D012313:RNA / Q000235:genetics; D012322:RNA Precursors / Q000235:genetics*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*
D012322:RNA Precursors; C034477:RNA primers; D011061:Poly A; C032883:cytidine 3',5'-diphosphate; D003565:Cytidine Diphosphate; D012313:RNA; D010047:Ovalbumin; D004247:DNA; D012194:RNA-Directed DNA Polymerase