title: The application of immunoperoxidase staining for the detection of causative fungi in tissue specimens of mycosis I.
abstract: This study was performed in order to identify the fungi of four species (Aspergillus fumigatus, Fusarium anthophilum, Candida albicans, Cryptococcus neoformans) in formalin-fixed, paraffin-embedded tissue sections by the indirect method of immunoperoxidase staining. Mature albino rabbits were immunized by formalin-killed organisms. The antibodies were prepared by precipitation at a 50% saturation of ammonium sulfate and were checked for cross-reactivities by Ouchterlony's double immunodiffusion and precipitin test. The immunoperoxidase staining was applied to the paraffin-embedded tissue sections of infected mice, human autopsy and biopsy specimens. Although each fungus was stained clearly the cell wall, cross-reactivities appeared among them, however it was possible to identify four fungi by absorption and dilution of the antisera.
authors: Department of Plastic Surgery, Nagasaki University School of Medicine, Japan.
authors: K
authors: K
authors: Kobayashi
authors: M
authors: M
authors: Hayama
authors: M
authors: M
authors: Hotchi
mesh_terms: D000818:Animals; D001228:Aspergillosis / Q000175:diagnosis; D001232:Aspergillus fumigatus; D002177:Candidiasis / Q000175:diagnosis; D003429:Cross Reactions; D003453:Cryptococcosis / Q000175:diagnosis; D005670:Fusarium; D007106:Immune Sera; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D051379:Mice; D009181:Mycoses / Q000175:diagnosis*; D011817:Rabbits / Q000276:immunology; D013194:Staining and Labeling
chemical_list: D007106:Immune Sera