title: Effect of topical capsaicin on the cutaneous responses to inflammatory mediators and to antigen in man.
abstract: Topical capsaicin pretreatment is known to deplete cutaneous sensory nerves of neuropeptides. We have assessed the effect of topical capsaicin pretreatment on the responses to intradermal injections of histamine and platelet-activating factor (PAF) in six normal subjects, and of prostaglandin E2, histamine, and antigen in 10 atopic subjects. Capsaicin pretreatment caused significant inhibition of the immediate flare response to histamine in both normal (19.8 +/- 2.6 to 7.3 +/- 2.9 cm2 at 5 minutes; p less than 0.01) and atopic subjects (16.5 +/- 1.4 to 10.3 +/- 1.9 cm2 at 5 minutes; p less than 0.01). The PAF-induced flare was also inhibited from 12.2 +/- 2.9 to 2.7 +/- 1.6 cm2 at 5 minutes after injection (p less than 0.01). In contrast, capsaicin pretreatment did not significantly alter the flare responses to prostaglandin E2 or antigen in atopic subjects. The acute wheal responses to all stimuli were unchanged, as was the late-phase response to antigen. These results support the hypothesis that the cutaneous vasodilator effect of histamine and PAF may be mediated by a local axon reflex involving the release of neuropeptides from sensory nerves. A consistent effect of capsaicin pretreatment on the flare response induced by endogenous mediators released during a cutaneous IgE-mediated response was not observed. Increases in microvascular permeability and the late-phase response to antigen are independent of neuropeptide release from cutaneous nerves.
authors: Department of Thoracic Medicine, National Heart and Lung Institute, Brompton Hospital, London, England.
authors: M T
authors: MT
authors: McCusker
authors: K F
authors: KF
authors: Chung
authors: N M
authors: NM
authors: Roberts
authors: P J
authors: PJ
authors: Barnes
mesh_terms: D000287:Administration, Topical; D000328:Adult; D000485:Allergens; D002211:Capsaicin; D015232:Dinoprostone / Q000276:immunology*; D004311:Double-Blind Method; D006636:Histamine Release; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis* / Q000276:immunology; D006969:Hypersensitivity, Immediate / Q000175:diagnosis* / Q000276:immunology; D009479:Neuropeptides / Q000276:immunology; D010972:Platelet Activating Factor / Q000276:immunology*
chemical_list: D000485:Allergens; D009479:Neuropeptides; D010972:Platelet Activating Factor; D015232:Dinoprostone; D002211:Capsaicin