[Abolition of the inhibitory effect of antibodies against S-100 proteins on the calcium current of molluscan neurons after intracellular injection of EGTA].
A two-electrode voltage clamp method was used to study Ca inward current (ICa) in unidentified isolated snail neurons. Extracellular administration of antibodies against S-100 proteins (AS-100) produces a reversible inhibition of ICa in a dose-dependent and time-dependent manner. Intracellular injection of EGTA with the help of 10nA 5-10 min microiontophoresis through a multibarrel microelectrode evoked a weak increase in the amplitude of the ICa before AS-100 application and a complete abolition of the inhibitory effect of AS-100 on the ICa. The data obtained suggest that AS-100 application results in an increase in the intracellular Ca concentration and Ca-dependent inactivation of Ca-channels.
Solntseva
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D000818:Animals; D000906:Antibodies / Q000008:administration & dosage*; D002118:Calcium / Q000378:metabolism / Q000502:physiology*; D004533:Egtazic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D007473:Ion Channels; D008564:Membrane Potentials; D008839:Microelectrodes; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D009418:S100 Proteins / Q000276:immunology*; D012908:Snails
D000906:Antibodies; D007473:Ion Channels; D009418:S100 Proteins; D004533:Egtazic Acid; D002118:Calcium
Ustranenie ingibiruiushchego éffekta antitel k belkam S-100 na kal'tsievyĭ tok neĭronov molliuska pri vnutrikletochnoĭ in"ektsii EGTA.