[The effect of 5-azacytidine on the cell cycle and chromosome structure in cell cultures of swine embryonal kidney tissue].
Incubation of pig kidney cells (PK-cells) in the presence of 5-azacytidine (5-azaC), a DNA enzymatic methylation inhibitor, at concentration of 20 microM for 6 or 24 h results in a dramatic decrease in the DNA methylation level (5mC/C + m5.100) - from 3.0 in control to 1.0 in experiment. This is accompanied by a virtually complete arrest of mitosis and a decrease in the ratio of labeled interphase cells upon simultaneous introduction of 3H-deoxycytidine. The incubation with 5-azaC block PK-cells mainly in the G2-period. The inhibitory action is reversible, for the cells enter into mitosis after removal of the inhibitor. Metaphase chromosomes, whose DNA was replicated in the presence of the 5-azaC, exhibit certain ultrastructural differences from normal ones. The results are being discussed in connection with the earlier data on the anomalous structure of interphase chromatin formed in the course undermethylated DNA replication.
Zatsepina
O V
OV
Aĭrapetian
V O
VO
Kir'ianov
G I
GI
D000818:Animals; D001374:Azacitidine / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002843:Chromatin / Q000648:ultrastructure; D002875:Chromosomes / Q000648:ultrastructure*; D046508:Culture Techniques; D004247:DNA / Q000032:analysis / Q000187:drug effects; D004261:DNA Replication / Q000187:drug effects; D007668:Kidney; D008745:Methylation; D008940:Mitotic Index / Q000187:drug effects; D013552:Swine
D002843:Chromatin; D004247:DNA; D001374:Azacitidine
Vliianie 5-azatsitidina na kletochnyĭ na kletochnyĭ tsikl i strukturkhromosom v kletdu kul'tury tkani pochki émbriona svin'i.