Human monocyte chemoattractant protein-1 (MCP-1). Full-length cDNA cloning, expression in mitogen-stimulated blood mononuclear leukocytes, and sequence similarity to mouse competence gene JE.
The purpose of this work was to analyze cDNA encoding human monocyte chemoattractant protein-1 (MCP-1), previously isolated from glioma cell line culture fluid. Screening of a cDNA library from total poly(A) RNA of glioma cell line U-105MG yielded a clone that coded for the entire MCP-1. Nucleotide sequence analysis and comparison with the amino acid sequence of purified MCP-1 showed that the cDNA clone comprises a 53-nucleotide 5'-non-coding region, an open reading frame coding for a 99-residue protein of which the last 76 residues correspond exactly to pure MCP-1, and a 389-nucleotide 3'-untranslated region. The hydrophobicity of the first 23 residues is typical of a signal peptide. Southern blot analysis of human and animal genomic DNA showed that there is a single MCP-1 gene, which is conserved in several primates. MCP-1 mRNA was induced in human peripheral blood mononuclear leukocytes (PBMNLs) by PHA, LPS and IL-1, but not by IL-2, TNF, or IFN-gamma. Among proteins with similar sequences, the coding regions of MCP-1 and mouse JE show 68% identity. This suggest that MCP-1 is the human homologue of the mouse competence gene JE.
Yoshimura
T
T
Laboratory of Immunobiology, National Cancer Institute, Frederick, MD 21701.
Yuhki
N
N
Moore
S K
SK
Appella
E
E
Lerman
M I
MI
Leonard
E J
EJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D018932:Chemokine CCL2; D002630:Chemotactic Factors / Q000235:genetics*; D002634:Chemotaxis, Leukocyte; D003001:Cloning, Molecular; D004247:DNA / Q000097:blood / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D005910:Glioma; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009000:Monocytes / Q000502:physiology*; D011061:Poly A / Q000235:genetics; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D012689:Sequence Homology, Nucleic Acid
D018932:Chemokine CCL2; D002630:Chemotactic Factors; D004273:DNA, Neoplasm; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA