Characterization of urinary keratin number 18 using a new assay.
The levels of the low-molecular weight keratin 18 appearing in the voided urine of 130 healthy volunteers was studied using a new immunoradiometric assay for keratin 18 (IRMAK-18). Keratin 18 was detected in 79 urines with an average level of 2.5 +/- 4.7 ng./ml. The assay does not detect complex epidermal keratins or non-keratin intermediate filaments. Only 50% of the purified keratin added to urine could be recovered. The recovered keratin had the same molecular weight as the input molecules, implying that substances in the urine physically inhibited the assay rather than destroyed the keratin. The independent variables of cell count, dead cells, and protein concentration were not found to be important in evaluating the results. However, the urine concentration, as measured by creatinine, might be an important consideration for assessing the levels of keratin detected.
Rossitto
P V
PV
Department of Pathology, University of California School of Medicine, Davis 95616.
Chan
R
R
Strand
M A
MA
Miller
C H
CH
Baker
W C
WC
Deitch
A D
AD
deVere White
R
R
Cardiff
R D
RD
D000328:Adult; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007633:Keratins / Q000652:urine*; D011874:Radiometry
D007633:Keratins