Effects of dexamethasone and insulin on the acute phase response of Morris hepatoma cells and of rat hepatocytes in culture.
Dexamethasone and insulin stimulate production of several plasma proteins in primary cultures of adult rat hepatocytes but inhibit their production in primary cultures of Morris hepatoma cell line 7777W. The acute phase response elicited in cultured cells by crude cytokines from activated rat peritoneal macrophages is considerably higher in hepatocytes in the presence of hormones, and especially of dexamethasone. In hepatoma cells the hormones enhance the cytokine-induced formation of fibrinogen and cysteine proteinase inhibitor but are without significant effect on suppression of albumin and alpha-fetoprotein synthesis by macrophage supernatants.
Magielska-Zero
D
D
Department of Animal Biochemistry, Jan Zurzycki Institute of Molecular Biology, Jagiellonian University, Kraków, Poland.
Guzdek
A
A
Bereta
J
J
Kurdowska
A
A
Cieszka
K
K
Koj
A
A
D000209:Acute-Phase Proteins / Q000096:biosynthesis*; D000818:Animals; D001685:Biological Factors / Q000494:pharmacology; D002478:Cells, Cultured; D016207:Cytokines; D003907:Dexamethasone / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D008099:Liver / Q000166:cytology; D008114:Liver Neoplasms, Experimental / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D014407:Tumor Cells, Cultured / Q000187:drug effects*
D000209:Acute-Phase Proteins; D001685:Biological Factors; D016207:Cytokines; D007328:Insulin; D003907:Dexamethasone