Identification of neutrophil alkaline phosphatase-inducing factor in cystic fluid of a human squamous cell carcinoma as granulocyte colony-stimulating factor.
We have previously shown that a factor termed neutrophil alkaline phosphatase-inducing factor (NAP-IF) has the capacity to induce neutrophil alkaline phosphatase (NAP) in postmitotic granulocytes (PMGs). This factor has characteristics similar to those of granulocyte colony-stimulating factor (G-CSF), suggesting that the two factors assayed by different methods may be attributable to an identical macromolecule. In a preliminary experiment, we showed that purified natural G-CSF (nG-CSF) could induce NAP in vitro in the presence of 10% (v/v) fetal calf serum (FCS). In this study, purified human nG-CSF and recombinant G-CSF (rG-CSF) induced NAP in granulocytes from both normal individuals and patients with chronic myelogenous leukemia in a dose-dependent fashion in serum-free and serum-containing culture conditions. The induction of NAP by G-CSF was detectable at 0.4 ng/ml and became maximal between 10 and 20 ng/ml. Anti-G-CSF serum incubated with either NAP-IF or rG-CSF inhibited induction of NAP. Morphological examinations revealed that granulocytes cultured with G-CSF were more mature than those cultured without G-CSF, indicating that G-CSF promoted maturation of granulocytes in parallel with NAP induction. These results indicate that NAP-IF in the cystic fluid of a human squamous cell carcinoma is identical to G-CSF and that induction of NAP by G-CSF is really a reflection of cell maturation promoted by G-CSF.
Sato
N
N
Third Department of Internal Medicine, National Defense Medical College, Saitama, Japan.
Asano
S
S
Koeffler
H P
HP
Yoshida
S
S
Takaku
F
F
Takatani
O
O
D000469:Alkaline Phosphatase / Q000096:biosynthesis*; D002294:Carcinoma, Squamous Cell / Q000032:analysis*; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000032:analysis / Q000494:pharmacology*; D003560:Cysts / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005122:Exudates and Transudates / Q000032:analysis*; D016179:Granulocyte Colony-Stimulating Factor; D006801:Humans; D009504:Neutrophils / Q000201:enzymology*
D003115:Colony-Stimulating Factors; D016179:Granulocyte Colony-Stimulating Factor; D000469:Alkaline Phosphatase