[Antigenic polysaccharides of bacteria. 35. Establishment of the structure of polysaccharide chains of lipopolysaccharides of Pseudomonas cepacia IMV 4176 and IMV 4202 (Serotype 3)].
On mild acid degradation of the Pseudomonas cepacia strain IMV 4176 lipopolysaccharide, two polysaccharides were obtained, one of which is a homopolymer of N-acetyl-D-galactosamine and the other is composed of equal amounts of N-acetyl-D-galactosamine and D-ribose. Partial hydrolysis with aqueous oxalic acid caused depolymerization of the heteropolysaccharide, and the homopolysaccharide was isolated in the individual state. On the basis of methylation and 13C NMR analysis, it was concluded that both polysaccharides are built up of disaccharide repeating units having the following structures: ----4)-alpha-D-GalpNAc-(1----4)-beta-D-GalpNAc-(1---- and ----4)-alpha-D-GalpNAc-(1----2)-beta-D-Ribf-(1----. The heteropolysaccharide from P. cepacia strain 4176 is identical by the structure of the repeating unit to the O-specific polysaccharide of P. cepacia strain IMV 4202 (serotype 3), Pseudomonas aeruginosa O12 and Serratia marcescens O14.
Knirel'
Iu A
IuA
Tanamar
N V
NV
Soldatkina
M A
MA
Zakharova
I Ia
IIa
D000942:Antigens, Bacterial / Q000032:analysis*; D002240:Carbohydrate Sequence; D006386:Hemagglutination Tests; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D019081:O Antigens; D011549:Pseudomonas / Q000032:analysis / Q000276:immunology*; D013045:Species Specificity
D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D019081:O Antigens
Antigennye polisakharidy bakteriĭ. 35. Ustanovlenie struktury polisakharidnykh tsepeĭ lipopolisakkharidov Pseudomonas cepacia IMV 4176 i IMV 4202 (serotip 3).