title: Malignant peripheral nerve sheath tumors and spindle cell sarcomas: an immunohistochemical analysis of multiple markers.
abstract: An immunocytochemical study using a panel of commercially available antisera, has been performed to distinguish on the basis of their immunoreactivity a series of spindle cell sarcomas diagnosed solely on the histologic features: 11 malignant schwannomas (MS), 8 leiomyosarcomas (LMS) and 3 malignant fibrous histiocytomas (MFH). The results have been compared with those obtained in 12 benign and 8 malignant peripheral nerve sheath tumors (MPNST) in which the microscopic diagnosis was supported by their origin in a nerve trunk and/or in von Recklinghausen's (vR) disease. The following antisera were used: anti-S-100 protein, anti-Leu-7, anti-neuron specific enolase (NSE), anti-myelin basic protein (MBP), anti-glial fibrillary acidic protein (GFAP) and anti-actin. S-100 protein was present in 100% of benign and malignant peripheral nerve tumors and in 7/11 (63%) of MS diagnosed on histological basis only and in 3/8 (37%) LMS. MFH were negative. Leu-7 positivity was observed in 8/12 (66%) and 6/8 (75%), respectively, in benign and malignant PNS neoplasms, in 5/11 (45%) MS, 4/8 (50%) LMS and 2/3 (66%) MFH. NSE was present in 7/12 (58%) and 6/8 (75%), respectively, in benign and malignant PNS tumors, in 6/11 (54%) MS and in 1/8 (12%) LMS. MFH were negative. MBP resulted negative in peripheral nerve neoplasms and spindle cell sarcomas. GFAP positivity was observed in 2/12 (16%) and 1/8 (12%), respectively, in benign and malignant PNS neoplasms. All spindle cell sarcomas were negative. All cases of MPNST and spindle cell sarcomas showed actin immunoreactivity. These results indicate that: (1) MBP, Leu-7 and NSE do not represent markers of schwannian differentiation; (2) GFAP, although rarely expressed, may indicate schwannian differentiation, and (3) malignant peripheral nerve neoplasms and LMS share immunoreactivity for S-100, Leu-7, NSE and actin, therefore they cannot be differentiated on immunocytochemical basis using commercially available antisera.
authors: Istituto di Anatomia ed Istologia Patologica, Università di Bologna, Italia.
authors: F
authors: F
authors: Giangaspero
authors: F C
authors: FC
authors: Fratamico
authors: C
authors: C
authors: Ceccarelli
authors: M
authors: M
authors: Brisigotti
mesh_terms: D000199:Actins / Q000032:analysis; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000943:Antigens, Differentiation / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis*; D018959:CD57 Antigens; D002648:Child; D002675:Child, Preschool; D003937:Diagnosis, Differential; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D018219:Histiocytoma, Benign Fibrous / Q000032:analysis* / Q000175:diagnosis / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007890:Leiomyosarcoma / Q000032:analysis / Q000175:diagnosis / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009442:Neurilemmoma / Q000032:analysis* / Q000175:diagnosis / Q000473:pathology; D010524:Peripheral Nervous System Neoplasms / Q000032:analysis* / Q000175:diagnosis / Q000473:pathology; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D009418:S100 Proteins / Q000032:analysis; D012509:Sarcoma / Q000032:analysis* / Q000175:diagnosis / Q000473:pathology
chemical_list: D000199:Actins; D000943:Antigens, Differentiation; D014408:Biomarkers, Tumor; D018959:CD57 Antigens; D005904:Glial Fibrillary Acidic Protein; D009418:S100 Proteins; D010751:Phosphopyruvate Hydratase