The prostate-specific antigen gene and the human glandular kallikrein-1 gene are tandemly located on chromosome 19.
Using a prostate-specific antigen cDNA as a hybridization probe, clones containing the kallikrein genes encoding prostate-specific antigen, human glandular kallikrein-1 and pancreas/kidney kallikrein were isolated from a human genomic library. Clones containing the prostate-specific antigen gene and the human glandular kallikrein-1 gene overlap and span a region of about 36 kb. The two genes are aligned in a head to tail orientation at a mutual distance of 12 kb. Southern blot analysis of DNA from a panel of human-hamster hybrid cells with specific probes revealed the genes to be situated on chromosome 19. Assuming that the pancreas/kidney kallikrein gene is located in the same cluster, the distance to the prostate-specific antigen gene and the human glandular kallikrein gene must be at least 15 kb.
Riegman
P H
PH
Department of Pathology, Erasmus University, Rotterdam, The Netherlands.
Vlietstra
R J
RJ
Klaassen
P
P
van der Korput
J A
JA
Geurts van Kessel
A
A
Romijn
J C
JC
Trapman
J
J
D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics*; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19; D003001:Cloning, Molecular; D006224:Cricetinae; D015342:DNA Probes; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII; D006801:Humans; D006822:Hybrid Cells; D007610:Kallikreins / Q000235:genetics*; D007668:Kidney / Q000201:enzymology; D008297:Male; D009693:Nucleic Acid Hybridization; D010179:Pancreas / Q000201:enzymology; D011467:Prostate; D017430:Prostate-Specific Antigen; D020840:Tissue Kallikreins
D000951:Antigens, Neoplasm; D015342:DNA Probes; D015245:Deoxyribonuclease BamHI; D015247:Deoxyribonuclease HindIII; D007610:Kallikreins; D020840:Tissue Kallikreins; D017430:Prostate-Specific Antigen