Calcium and the production of interferon by human peripheral blood mononuclear cells.
We have studied the ability of human peripheral blood mononuclear cells (PBMC) to produce interferon-alpha (IFN-alpha) and IFN-gamma in the presence of pharmacologic agents known to influence calcium transport or calcium-dependent processes. We have found that the production of human (Hu) IFN-gamma is affected significantly by alterations in calcium flux; however, this influence is dependent upon the nature of the compound used to induce IFN. Inhibitors of protein kinase C decreased yields of IFN-gamma but inhibition of calmodulin did not. The presence of vitamin D3 reduced IFN-gamma titers when PHA and IL-2 were used to induce IFN, but not when ionomycin was used as the inducer. The production of IFN-gamma by PBMC was reduced by diminished concentrations in extracellular calcium but not extracellular magnesium. In contrast, neither the presence of any of the pharmacological agents tested above nor the reduction of the calcium concentration influenced the production of HuIFN-alpha by PBMC.
Cesario
T C
TC
Department of Medicine, University of California-Irvine 92668.
McCloskey
M
M
Carandang
G
G
Yousefi
S
S
Chiu
J
J
Vaziri
N
N
D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D004789:Enzyme Activation; D004987:Ethers / Q000494:pharmacology; D006801:Humans; D007369:Interferon Inducers / Q000494:pharmacology; D007370:Interferon Type I / Q000096:biosynthesis*; D007371:Interferon-gamma / Q000096:biosynthesis*; D015759:Ionomycin; D007476:Ionophores / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D009543:Nifedipine / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors
D004987:Ethers; D007369:Interferon Inducers; D007370:Interferon Type I; D007476:Ionophores; D000001:Calcimycin; D015759:Ionomycin; D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; D007371:Interferon-gamma; D011493:Protein Kinase C; D009543:Nifedipine; D002118:Calcium