Sudden outbreak of a leukemia-like lesion in female CBA mice after repeated injections of 5-azacytidine.
A total of 11 i.p. injections of 1 mg/kg 5-azacytidine given within 21 weeks induced a sudden outbreak of a leukemia-like disease in female CBA mice about 2 weeks after the last injection. The outbreak of disease was highly synchronous; 68% of animals who had survived the treatment period succumbed within a period of 5 days, 2/3 of them on a single day. The lesion had a characteristic form commonly presenting as a result of hemorrhagic effusion in the thoracic and/or abdominal cavities, associated with diffuse infiltration of lymphatic and fatty tissue by lymphoblasts. It appears that a critical combination of factors was responsible for the unexpected appearance of the lesion. Female BALB/c mice treated identically were not affected. The factors indicating that the lesion was induced by an epigenetic mechanism are discussed.
Luz
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Institut für Pathologie, Gesellschaft für Strahlen- und Umweltforschung (GSF), Neuherberg/München, Federal Republic of Germany.
Murray
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D000818:Animals; D001374:Azacitidine / Q000633:toxicity*; D004196:Disease Outbreaks; D005260:Female; D007942:Leukemia, Experimental / Q000139:chemically induced* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D013997:Time Factors
D001374:Azacitidine