Alterations of pancreatic juice amylase by glucocorticoid levels in the rat.
By use of isoelectrofocusing, three isoenzymes with pIs of 8.40, 8.55, and 8.65 were characterized in the amylase fraction of rat pancreatic juice. Enzyme secretion in rat exocrine pancreas is affected by glucocorticoid levels; adrenalectomy led to a significant decrease in protein secretion which was more pronounced in the amylase fraction, in which the isoenzymes with pI 8.55 and 8.65 disappeared. Substitution therapy with hydrocortisone (25 mg/kg/day, for 6 days) restored exocrine pancreatic secretion to almost normal levels. Administration of hydrocortisone to control rats led to structural alterations in enzymes secreted, splitting the amylase isoenzymes with pI 8.40; this was confirmed by crossed immunoelectrophoresis. It is concluded that glucocorticoid levels play an important role in the maintenance of function of exocrine pancreas and it is suggested that, although hydrocortisone fulfills the objective of restoring enzyme secretion diminished by adrenalectomy, it is possible that intensive treatment could have undesirable effects on the structure of enzymes and could involve pancreatic disfunctionality.
de Dios
I
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Departamento de Fisiología y Farmacología, Facultad de Biología, Universidad de Salamanca, Spain.
Manso
M A
MA
Calvo
J J
JJ
D000315:Adrenalectomy; D000681:Amylases / Q000378:metabolism*; D000818:Animals; D006854:Hydrocortisone / Q000494:pharmacology*; D007123:Immunoelectrophoresis, Two-Dimensional; D008297:Male; D010189:Pancreatic Juice / Q000187:drug effects / Q000201:enzymology*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D000516:alpha-Amylases / Q000378:metabolism
D011506:Proteins; D000681:Amylases; D000516:alpha-Amylases; D006854:Hydrocortisone