title: Molecular cloning of human myelin-associated glycoprotein.
abstract: The nucleotide sequence for human myelin-associated glycoprotein (MAG) and its deduced amino acid sequence, obtained by analysis of two overlapping cDNA clones isolated from a human brain cDNA library, is presented and compared to that reported for rat MAG. The sequence provides an open reading frame of 1,878 nucleotides encoding a peptide of 626 amino acids with a calculated molecular weight of 69.1 kD. It is 89% homologous in nucleotide sequence to the large isoform of rat MAG, with 95% homology in the amino acid sequence. It contains 9 potential glycosylation sites, one more than in rat, and shares other key features with rat MAG, including 5 immunoglobulin-like regions of internal homology, an RGD sequence, and potential phosphorylation sites. Its structure appears to be highly conserved in evolution, possibly suggesting a close interdependence between its structure and function. The human gene is located on the proximal long arm of chromosome 19 (19q12----q13.2).
authors: Department of Neurology, College of Physician and Surgeons, Columbia Presbyterian Medical Center, New York, NY 10032.
authors: G
authors: G
authors: Spagnol
authors: M
authors: M
authors: Williams
authors: J
authors: J
authors: Srinivasan
authors: J
authors: J
authors: Golier
authors: D
authors: D
authors: Bauer
authors: R V
authors: RV
authors: Lebo
authors: N
authors: N
authors: Latov
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009185:Myelin Proteins / Q000378:metabolism*; D018992:Myelin-Associated Glycoprotein
chemical_list: D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D004247:DNA