title: Precipitation of soluble affinity complexes by a second affinity interaction: a model study.
abstract: Lactate dehydrogenase was purified by affinity precipitation. The enzyme bound to Blue Dextran (the Cibacron blue residues) and was precipitated by addition of concanavalin A. The lectin functions as a crosslinking agent, building up large flocs of dextran that subsequently precipitate, thus co-precipitating the affinity-bound lactate dehydrogenase.
authors: Department of Biotechnology, University of Lund, Sweden.
authors: C
authors: C
authors: Senstad
authors: B
authors: B
authors: Mattiasson
mesh_terms: D000818:Animals; D011232:Chemical Precipitation; D004396:Coloring Agents; D003208:Concanavalin A; D003911:Dextrans; D004591:Electrophoresis, Polyacrylamide Gel; D007770:L-Lactate Dehydrogenase / Q000302:isolation & purification* / Q000378:metabolism; D009206:Myocardium / Q000201:enzymology; D011506:Proteins / Q000032:analysis; D012995:Solubility; D013552:Swine
chemical_list: D004396:Coloring Agents; D003911:Dextrans; D011506:Proteins; D003208:Concanavalin A; C017080:blue dextran; D007770:L-Lactate Dehydrogenase