Novel immunochemical characteristics of glutathione S-transferase isozymes of rat liver cytosol.
Reevaluation of the immunochemical relationships among the individual glutathione S-transferase (GST) isozymes (GSTs 1-1, 1-2, 2-2, 3-3, 3-4, 4-4, and GSTs with isoelectric points of 7.5 and 6.8) of rat liver cytosol was performed utilizing the immunoblot technique. As a result, we found that the respective isozymes of two isozyme classes of rat liver cytosol might possess a common epitope(s) which has been undetected by the Ouchterlony double-diffusion method. The assumption was further supported by the results of the effects of Fab' prepared from some anti-GST antibodies on the enzymatic activity of GSTs.
Fukai
F
F
Department of Patho-Physiology, Faculty of Pharmaceutical Sciences, Science University of Tokyo, Japan.
Kurano
M
M
Nishizawa
S
S
Kiire
Y
Y
Nagai
T
T
Katayama
T
T
D000818:Animals; D000906:Antibodies / Q000276:immunology; D003600:Cytosol / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D005982:Glutathione Transferase / Q000276:immunology / Q000302:isolation & purification*; D015151:Immunoblotting; D007120:Immunochemistry; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007527:Isoenzymes / Q000276:immunology / Q000302:isolation & purification*; D008099:Liver / Q000201:enzymology*; D051381:Rats
D000906:Antibodies; D000939:Epitopes; D007140:Immunoglobulin Fab Fragments; D007527:Isoenzymes; D005982:Glutathione Transferase