Monoclonal antibodies against human immunodeficiency virus (HIV) type 2 core proteins: cross-reactivity with HIV type 1 and simian immunodeficiency virus.
Four mouse monoclonal antibodies were developed after immunization with one human immunodeficiency virus (HIV) type 2 isolate and were tested for reactivity with different HIV-1, HIV-2, and simian immunodeficiency virus (SIV) isolates in an immunofluorescence assay and by immunological blot analysis. One of them, an anti-capsid (p24) antibody, called R1C7, reacted with all HIV-1, HIV-2, and SIV isolates tested, thus identifying an epitope shared by all HIV and SIV. Another anti-capsid antibody, named A4F6, reacted with three HIV-2 isolates (HIV-2NIH-Z, LAV-2Rod, and LK001 ST9), some SIV isolates (STLV-IIIAGM, SIV-251, and SIV-309), but no HIV-1 isolates. Two anti-matrix (p16) antibodies, named R5C4 and R5F6, reacted strongly only with the HIV-2 isolates. The use of these monoclonal antibodies for rapid discrimination and identification of acquired immunodeficiency syndrome-related retroviruses is discussed.
Minassian
A A
AA
Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, MD 20892.
Kalyanaraman
V S
VS
Gallo
R C
RC
Popovic
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D002213:Capsid / Q000276:immunology; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis; D006678:HIV / Q000276:immunology*; D016655:HIV Core Protein p24; D000882:Haplorhini; D051379:Mice; D008992:Monkey Diseases / Q000382:microbiology; D012190:Retroviridae / Q000276:immunology*; D012191:Retroviridae Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D016655:HIV Core Protein p24; D012191:Retroviridae Proteins