False-positive sera do not react with human immunodeficiency virus (HIV) gag-encoded recombinant antigen.
Ten sera from healthy blood donors positive by enzyme-linked immunoadsorbent assay (ELISA) were studied by immunoblot assay using natural and recombinant proteins. They interacted only with p17 or p24 proteins but were nonreactive with a recombinant protein (RP 50), which carries antigenic determinants to p17 and p24. Reactions were not blocked by preincubation of sera with genetically engineered p17 and p24 or purified viral p24, indicating that some new epitopes were formed during the Western blot procedure. Recombinant gag-encoded protein is required for confirmation of human immunodeficiency virus (HIV) seropositivity.
Bukrinsky
M I
MI
D.I. Ivanovski Institute of Virology, Moscow, Union of Soviet Socialist Republics.
Syrtsev
V A
VA
Popov
S A
SA
Barsov
E V
EV
Chaplinskas
S A
SA
Karamov
E V
EV
D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D005189:False Positive Reactions; D015683:Gene Products, gag; D005814:Genes, Viral; D006678:HIV / Q000235:genetics / Q000276:immunology*; D015488:HIV Antigens / Q000235:genetics / Q000276:immunology*; D016655:HIV Core Protein p24; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D012191:Retroviridae Proteins / Q000235:genetics / Q000276:immunology*; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus
D000939:Epitopes; D015683:Gene Products, gag; D015488:HIV Antigens; D016655:HIV Core Protein p24; D007106:Immune Sera; D011994:Recombinant Proteins; D012191:Retroviridae Proteins; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; C054858:p17 protein, Human Immunodeficiency Virus Type 1