Co-localization to chromosome bands 99E1-3 of the Drosophila melanogaster myosin light chain-2 gene and a haplo-insufficient locus that affects flight behavior.
Using overlapping synthetic deficiencies, we find that a haplo-insufficient locus affecting flight behavior and the myosin light chain-2 gene co-map to the Drosophila melanogaster polytene chromosome interval 99D9-E1 to 99E2-3. From screening over 9000 EMS-treated chromosomes, we obtained alleles of two complementation groups that map to this same interval. One of these complementation groups lfm(3)99Eb, exhibits dominant flightless behavior; thus, flightless behavior of the deficiency is in all likelihood due to hemizygosity of this single locus. Rescue of flightless behavior by a duplication indicates that the single allele, E38, of the Ifm(3)99Eb complementation group is a hypomorph. Based upon its map position and a reduction in concentration of myosin light chain-2 mRNA in heterozygotes, we propose that Ifm(3)Eb(E38) is a mutant allele of the myosin light chain-2 gene. Our genetic analysis also resulted in the identification of four dominant flightless alleles of an unlinked locus, l(3)nc99Eb, that exhibits dominant lethal synergism with Ifm(3)99Eb.
Warmke
J W
JW
Department of Molecular Genetics, Ohio State University, Columbus 43210.
Kreuz
A J
AJ
Falkenthal
S
S
D000483:Alleles; D000818:Animals; D002871:Chromosome Banding; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D004331:Drosophila melanogaster / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005426:Flight, Animal; D005799:Genes, Dominant; D005804:Genes, Lethal; D005816:Genetic Complementation Test; D008854:Microscopy, Electron; D008970:Molecular Weight; D005810:Multigene Family; D009132:Muscles / Q000032:analysis; D009154:Mutation; D015879:Myosin Subfragments; D009218:Myosins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010446:Peptide Fragments / Q000235:genetics*; D012313:RNA / Q000302:isolation & purification; D012469:Salivary Glands
D015879:Myosin Subfragments; D010446:Peptide Fragments; D012313:RNA; D009218:Myosins