A human lymphocyte homing receptor, the hermes antigen, is related to cartilage proteoglycan core and link proteins.
Lymphocyte interactions with high endothelial venules (HEV) during extravasation into lymphoid tissues involve an 85-95 kd class of lymphocyte surface glycoprotein(s), gp90Hermes (CD44). We report here the cloning of cDNA for gp90Hermes expressed in a mucosal HEV-binding B lymphoblastoid cell line, KCA. Northern hybridization revealed the presence of three invariant RNA bands at 1.5, 2.2, and 4.5 kb in mucosal HEV-, lymph node HEV-, or dual-binding cells. The deduced amino acid sequence predicts a mature protein with a C-terminal cytoplasmic tail, a hydrophobic transmembrane domain of 23 amino acids, and an N-terminal extracellular region of 248 amino acids. A proximal extracellular domain is the probable region of O-glycosylation and chondroitin sulfate linkage and displays at least two of the three immunodominant epitope clusters of native gp90Hermes. A distal region contains the majority of potential N-glycosylation sites and cysteines, and exhibits a striking homology to tandemly repeated domains of the cartilage link and proteoglycan core proteins. No significant similarities were found to the immunoglobulin, integrin, or cadherin gene families. Thus gp90Hermes represents a novel class of integral membrane protein involved in lymphocyte-endothelial cell interactions and lymphocyte homing.
Goldstein
L A
LA
Department of Pathology, Stanford University Medical Center, California 94305.
Zhou
D F
DF
Picker
L J
LJ
Minty
C N
CN
Bargatze
R F
RF
Ding
J F
JF
Butcher
E C
EC
D053674:Aggrecans; D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002448:Cell Adhesion; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification; D016326:Extracellular Matrix Proteins; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D006801:Humans; D037181:Lectins, C-Type; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011506:Proteins / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D011509:Proteoglycans; D012313:RNA / Q000032:analysis / Q000235:genetics; D011971:Receptors, Immunologic / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D016201:Receptors, Lymphocyte Homing; D012689:Sequence Homology, Nucleic Acid; D000099249:Proteoglycan Link Protein
D053674:Aggrecans; D004247:DNA; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D037181:Lectins, C-Type; D011506:Proteins; D011509:Proteoglycans; D012313:RNA; D011971:Receptors, Immunologic; D016201:Receptors, Lymphocyte Homing; D000099249:Proteoglycan Link Protein