A procedure for the small-scale isolation of plant RNA suitable for RNA blot analysis.
A small-scale method for the isolation of total RNA from plant tissue is described. The method provides RNA of suitable quantity and quality from 0.2 g fresh tissue for the detection of mRNA species by RNA blot analysis. The entire procedure is adapted to 1.5-ml microfuge tubes and takes less than 5 h. This method is well suited for the isolation of RNA from large numbers of samples or from samples of limited quantity.
Wadsworth
G J
GJ
Department of Genetics, North Carolina State University, Raleigh 27695-7614.
Redinbaugh
M G
MG
Scandalios
J G
JG
D015152:Blotting, Northern; D010944:Plants / Q000235:genetics*; D012313:RNA / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D014158:Transcription, Genetic; D003313:Zea mays / Q000235:genetics
D012333:RNA, Messenger; D012313:RNA