title: Secretion of glycoproteins through the cell wall of Candida albicans.
abstract: A monoclonal antibody raised against the pathogenic phase of Candida albicans has been coupled to colloidal gold and used to detect the corresponding epitope in cell wall and culture medium of blastoconidia grown as germ tubes in vitro. Immunogold silver staining of Western blots of culture supernatants demonstrated release of the epitope into the culture medium. The stain revealed 3 well defined bands of 205,000, 66,000 and 30,000 Mr and a smear from the top of the gel to an Mr of 120,000. Immunoelectron microscopy of ultrathin frozen sections of the corresponding growth forms showed that epitope accumulated first in the periplasmic space, generally corresponding to plasmalemma invaginations within the cytoplasm. From these sites, it was possible to follow continuous lines of epitope distribution through the cell wall and antigenic extrusion at the cell surface. In tangential sections of intensely labeled walls, these preferential excretion ways appeared to be organized as a parallel network. Antigen emergence at the cell surface corresponded to patches of material which tended to coalesce in an easily dissociated layer, probably corresponding to the fuzzy coat. These experiments demonstrate, for the first time, preferential ways for cellular secretion through the yeast cell wall.
authors: Unité INSERM 42, Biologie et Biochimie Parasitaires et Fongiques, Villeneuve d'Aseq/France.
authors: D
authors: D
authors: Poulain
authors: J C
authors: JC
authors: Cailliez
authors: J F
authors: JF
authors: Dubremetz
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis; D015153:Blotting, Western; D002176:Candida albicans / Q000378:metabolism* / Q000648:ultrastructure; D002473:Cell Wall / Q000378:metabolism / Q000648:ultrastructure; D000939:Epitopes / Q000032:analysis; D005656:Fungal Proteins / Q000276:immunology / Q000378:metabolism*; D006023:Glycoproteins / Q000276:immunology / Q000378:metabolism*; D007150:Immunohistochemistry; D008854:Microscopy, Electron
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D005656:Fungal Proteins; D006023:Glycoproteins