A common antigenic determinant of vimentin and desmin defined by monoclonal antibody.
A monoclonal antibody, VI-01, to vimentin and desmin has been isolated from a fusion of mouse myeloma cells with immune spleen cells. The antibody appeared specific for vimentin of various species and smooth muscle desmin and did not recognize other intermediate filament proteins when cell lines and tissue sections were assayed in immunofluorescence and cell lysates in immunoblotting. The binding test with purified vimentin and desmin confirmed these results. The antibody can differentiate carcinomas from sarcomas in human diagnostic surgical pathology.
Dráberová
E
E
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
Dráber
P
P
Havlícek
F
F
Viklický
V
V
D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D006224:Cricetinae; D003893:Desmin / Q000276:immunology*; D000939:Epitopes; D006801:Humans; D051379:Mice; D009130:Muscle, Smooth / Q000276:immunology; D051381:Rats; D013045:Species Specificity; D014746:Vimentin / Q000276:immunology*
D000911:Antibodies, Monoclonal; D003893:Desmin; D000939:Epitopes; D014746:Vimentin