Dextran determination in human serum with the aid of an enzymatic glucose method.
A method is presented for the determination of dextran concentrations. Following acid hydrolysis and partial neutralization, the resulting glucose is determined with the aid of glucose oxidase. Specificity is better than that of previously applied methods. Mean recovery is 100.2 +/- 1.6% (SD) for concentrations from 180 mg.l-1 to 6000 mg.l-1. Coefficients of variation are 2.2% for 240 mg.l-1, 1.2% for 600 mg.l-1 and 1.8% for 1200 mg.l-1. When used for volume measurements, the accuracy is 99.3 +/- 2.1% (SD) for volumes of 2.5 to 3 liters.
Gabel
L F
LF
Institute of Anaesthesiology, Catholic University of Nijmegen, The Netherlands.
Kerkkamp
H E
HE
Nederstigt
J N
JN
D003911:Dextrans / Q000097:blood*; D005947:Glucose; D006801:Humans; D013696:Temperature
D003911:Dextrans; D005947:Glucose