title: Herpes simplex virus type 1-induced modifications in the distribution of nucleolar B-36 protein.
abstract: Nucleolar B-36 protein was localized ultrastructurally by immunocytochemistry with monoclonal antibody P2G3 and colloidal gold label in rabbit fibroblast cells before and during infection with herpes simplex virus (HSV) type 1. In non-infected cells, labeling was sparse and restricted to the fibrillar component of the nucleoli. During the infectious cycle, B-36 protein appeared to be somewhat more abundant within the morphologically altered fibrillar component of the nucleoli. In addition, the protein was also detected in some but not all virus-induced intranuclear dense bodies. These observations suggest the presence of functionally distinct dense bodies. The association of B-36 protein with both structures was not disrupted by a hypotonic shock and detergent treatment, which suggest that these sites do not represent areas of passive intranuclear diffusion. Inhibition of protein synthesis late in infection, viral DNA replication or RNA synthesis did not alter the distribution of B-36 protein. We suggest that this protein may play a role in the increased compaction of the ribonucleoprotein fibrils induced by HSV infection, perhaps in association with some of the virus-encoded proteins which also have been detected in the nucleoli.
authors: Groupe de Laboratoires de l'Institut de Recherches Scientifiques sur le Cancer CNRS (ER 272), Villejuif/France.
authors: C
authors: C
authors: López-Iglesias
authors: F
authors: F
authors: Puvion-Dutilleul
authors: J
authors: J
authors: Cebrian
authors: M E
authors: ME
authors: Christensen
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002466:Cell Nucleolus / Q000648:ultrastructure; D002478:Cells, Cultured; D004279:DNA, Viral / Q000096:biosynthesis; D000939:Epitopes / Q000276:immunology; D005347:Fibroblasts / Q000502:physiology; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D002479:Inclusion Bodies / Q000648:ultrastructure; D009687:Nuclear Proteins / Q000032:analysis* / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis; D011817:Rabbits; D018139:Simplexvirus / Q000502:physiology*
chemical_list: D000911:Antibodies, Monoclonal; D004279:DNA, Viral; D000939:Epitopes; D009687:Nuclear Proteins; D012367:RNA, Viral; C057190:nucleolar B-36 protein, Oryctolagus cuniculus