[Purification of prostatic acid phosphatase and prostate specific antigen from human seminal plasma].
Prostatic acid phosphatase (PAP) and prostate specific antigen (PA), which are useful markers of diagnosis for prostatic cancer, have been purified from human seminal plasma. Total of 54.4 mg PAP and 3.0 mg PA were purified from 426.5 ml human seminal plasma. The purified antigens were demonstrated a single band by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), having a molecular weight of about 50,000 and 34,000, respectively. The PAP and PA purified from human seminal plasma were considered similar to that of prostatic tissue immunologically and biochemically.
Aoki
T
T
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Higashi-Nippon Gakuen University, Hokkaido, Japan.
Tanaka
T
T
Kataoka
S
S
Horikawa
H
H
Watabe
H
H
D000135:Acid Phosphatase / Q000302:isolation & purification*; D000328:Adult; D000951:Antigens, Neoplasm / Q000302:isolation & purification*; D014408:Biomarkers, Tumor / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008297:Male; D008970:Molecular Weight; D017430:Prostate-Specific Antigen; D012661:Semen / Q000032:analysis*
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D000135:Acid Phosphatase; D017430:Prostate-Specific Antigen