Effects of bovine respiratory disease viruses and isoprinosine on bovine leukocyte function in vitro.
Peripheral blood mononuclear cells obtained from 4- to 6-month-old-calves were inoculated in vitro with bovine herpesvirus-1, parainfluenza-3, or bovine virus diarrhea viruses. No increase in infectious virus progeny was observed; however, the viruses were detected in the cells for at least 96 h post-infection without any significant reduction in cell viability. The three viruses, either alone or in combination, suppressed phytohemagglutinin-induced proliferation of the mononuclear cells. The greatest suppression was observed in cultures inoculated with bovine virus diarrhea virus. Addition of isoprinosine partially restored this viral-induced suppression of proliferative response, and the efficiency of reversal was greater in bovine virus diarrhea virus-infected cells. Interleukin-2 activity was higher in cultures of virus-infected mononuclear cells than in cultures of non-infected cells.
Ghram
A
A
Department of Laboratory Medicine, Kansas State University, Manhattan 66506.
Reddy
P G
PG
Blecha
F
F
Minocha
H C
HC
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D001908:Diarrhea Viruses, Bovine Viral / Q000254:growth & development*; D007242:Herpesvirus 1, Bovine / Q000254:growth & development*; D007288:Inosine; D007542:Inosine Pranobex / Q000494:pharmacology*; D007376:Interleukin-2 / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects; D010224:Parainfluenza Virus 3, Human / Q000254:growth & development*; D010576:Pestivirus / Q000254:growth & development*; D018108:Respirovirus / Q000254:growth & development*; D010948:Viral Plaque Assay
D007376:Interleukin-2; D007288:Inosine; D007542:Inosine Pranobex