Binding of androgen-receptor complexes to alpha 2u-globulin genes and to the long terminal repeat of mouse mammary tumor virus.
The binding of androgen-receptor complexes to fragments derived from two alpha 2u-globulin genes (RAP 01 and RAO 01) was studied using a DNA-cellulose competition assay. Rat prostate cytosol labelled with [3H]mibolerone was used as a source of the androgen receptor. Two controls were included in these studies: the long terminal repeat (LTR) of mouse mammary tumor virus which has previously been shown to act as an androgen response element and a fragment of the C3 gene of prostatic binding protein which has been demonstrated to bind androgen-receptor complexes. Our experiments indicate that androgen-receptor complexes bind specifically and with comparable affinity to the C3 gene fragment, the LTR and a fragment of RAP 01 located in the 5'-upstream region (bp -642 up to -584). No specific interaction was observed with fragments derived from RAO 01. The region of RAP 01 which binds androgen-receptor complexes has previously been shown to interact with glucocorticoid receptors and contains a 17 bp sequence homologous with the consensus sequence for glucocorticoid-receptor binding. A mutation in this sequence in RAO 01 may be responsible for the loss of glucocorticoid and androgen-receptor binding. It is concluded that at least one member of the alpha 2u-globulin gene family interacts directly with androgen-receptor complexes with an affinity comparable to that observed for other androgen-dependent genes. The binding is observed in a region displaying also affinity for the glucocorticoid receptor.
Van Dijck
P
P
Laboratory for Experimental Medicine and Endocrinology, Onderwijs en Navorsing, Catholic University of Leuven, Belgium.
Winderickx
J
J
Heyns
W
W
Verhoeven
G
G
D000510:Alpha-Globulins / Q000235:genetics*; D000727:Androgen-Binding Protein / Q000235:genetics; D000818:Animals; D001667:Binding, Competitive; D002482:Cellulose / Q000031:analogs & derivatives; D004247:DNA; D004268:DNA-Binding Proteins / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics; D005810:Multigene Family; D009277:Nandrolone / Q000031:analogs & derivatives / Q000378:metabolism; D060789:Prostatein; D051381:Rats; D011919:Rats, Inbred Strains; D011944:Receptors, Androgen / Q000378:metabolism* / Q000502:physiology; D060765:Secretoglobins; D014598:Uteroglobin; D060788:Mammaglobin B
D000510:Alpha-Globulins; D000727:Androgen-Binding Protein; D002482:Cellulose; D004247:DNA; D004268:DNA-Binding Proteins; D009277:Nandrolone; D060789:Prostatein; D011944:Receptors, Androgen; D060765:Secretoglobins; D014598:Uteroglobin; C033190:DNA-cellulose; C511484:Scgb1d2 protein, rat; C511483:Scgb1d4 protein, rat; C013803:alpha 2u globulin; C100075:mibolerone; C556468:Scgb2a1 protein, rat; D060788:Mammaglobin B