Isolation and chromosomal localization of cDNAs encoding a novel human lymphocyte cell surface molecule, LAM-1. Homology with the mouse lymphocyte homing receptor and other human adhesion proteins.
A cDNA encoding a new human lymphocyte cell surface molecule has been isolated and shown to identify a fourth member of a recently discovered family of adhesion proteins. This lymphocyte-associated molecule (LAM-1) is uniquely composed of multiple distinct domains, one domain homologous with animal lectins, one homologous with epidermal growth factor, and two short consensus repeat units similar to those found in C3/C4 binding proteins. This cDNA clone hybridized with RNAs found in B cell lines and T lymphocytes, but not with RNA from other cell types. The amino acid sequence of LAM-1 is 77% homologous with the sequence of the mouse lymphocyte homing receptor, suggesting that LAM-1 may function in human lymphocyte adhesion. The LAM-1 gene is located on chromosome 1q23-25, as is another member of this adhesion family, suggesting that this new family of proteins may be encoded by a cluster of "adhesion protein" loci.
Tedder
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Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts.
Isaacs
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Ernst
T J
TJ
Demetri
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Adler
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Disteche
C M
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D000595:Amino Acid Sequence; D000818:Animals; D000954:Antigens, Surface / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D019041:L-Selectin; D008214:Lymphocytes / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011971:Receptors, Immunologic / Q000235:genetics*; D016201:Receptors, Lymphocyte Homing; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; D016201:Receptors, Lymphocyte Homing; D019041:L-Selectin; D004247:DNA