The maize En-1/Spm element transposes in potato.
The maize transposable element En-1 has been introduced into a diploid potato line via transformation with Agrobacterium tumefaciens. The element is transcriptionally active in potato. Numerous En specific RNAs are observed, including a 6 kb transcript characteristic of an active En-1 element in maize. In contrast to maize, where the 6.0 kb transcript is hardly detectable, this transcript is very abundant in the transgenic potato. Transposition of En-1 in the potato clone was analysed by Southern blot hybridization and confirmed by molecular isolation of En-1 excision and integration events. Sequence data of excision and integration sites revealed footprints and target site duplications similar to the ones described for En-1 in maize.
Frey
M
M
Max-Planck-Institut für Züchtungsforschung, Abteilung Molekulare Pflanzengenetik, Köln, Federal Republic of Germany.
Tavantzis
S M
SM
Saedler
H
H
D015139:Blotting, Southern; D002999:Clone Cells; D003001:Cloning, Molecular; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D010957:Plasmids; D012313:RNA / Q000096:biosynthesis / Q000235:genetics; D012231:Rhizobium / Q000235:genetics; D011198:Solanum tuberosum / Q000235:genetics*; D014158:Transcription, Genetic; D014170:Transformation, Genetic; D003313:Zea mays / Q000235:genetics*
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; C036483:T-DNA; D012313:RNA