title: Properties of single chloride channels in primary neuronal cultures of Drosophila.
abstract: An anion channel from Drosophila neurons had the selectivity sequence: NO3- (1.97) greater than Br- (1.12) approximately equal to I- (1.03) approximately equal to Cl- (1) greater than F- (0.32) much greater than glutamate (less than 0.02) as estimated by the permeability ratio based on the reversal potential measurement. 4-Acetoamido-4'-isothiocyanostilbene-2,2'-disulfonic acid applied internally at 10 microM blocked the channel. We suggest that the chloride channel identified here may provide a pathway for Cl- in the resting membrane of Drosophila neurons.
authors: Laboratory of Neurophysiology, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
authors: D
authors: D
authors: Yamamoto
authors: N
authors: N
authors: Suzuki
mesh_terms: D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000494:pharmacology; D000818:Animals; D000838:Anions; D002478:Cells, Cultured; D018118:Chloride Channels; D002712:Chlorides; D004330:Drosophila; D004553:Electric Conductivity; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D008564:Membrane Potentials; D008565:Membrane Proteins / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D010539:Permeability
chemical_list: D000838:Anions; D018118:Chloride Channels; D002712:Chlorides; D007473:Ion Channels; D008565:Membrane Proteins; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid