A rapid, quantitative assay for titration of bovine virus diarrhoea-mucosal disease virus.
An end point dilution microtitration assay is described that can be used for the titration of both cytopathic and non-cytopathic isolates of bovine virus diarrhoea-mucosal disease virus. Indirect immunofluorescence is used to detect infected MDBK cells in the wells of Terasaki plates. The virus titre is derived from the number of uninfected wells, using the Poisson distribution. The assay is simple, fast and economical. Titres of cytopathic virus determined by the microtitration assay and standard plaque assay are equivalent.
Roberts
P C
PC
Veterinary Research Laboratory, Montana State University, Bozeman 59717.
Etchison
J R
JR
Bond
C W
CW
D000818:Animals; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000175:diagnosis / Q000382:microbiology*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D001908:Diarrhea Viruses, Bovine Viral / Q000254:growth & development* / Q000302:isolation & purification; D010576:Pestivirus / Q000254:growth & development*; D013194:Staining and Labeling; D010948:Viral Plaque Assay