Expression of the mammalian Na+-independent L system amino acid transporter in Xenopus laevis oocytes.
System L is primarily responsible for the Na+-independent transport of neutral amino acids, those with bulky chains such as leucine, isoleucine, phenylalanine, etc., into mammalian cells. mRNA from rat kidney and human lymphoid cells, when microinjected into Xenopus laevis oocytes, induced expression of this transport system. The expressed transport exhibits characteristics similar to those reported for the System L amino acid transporter from a variety of mammalian cells. Injection of size-fractionated mRNA indicates that the System L transporter in both the rat kidney and human lymphoid cells is encoded by mRNA of about 3 to 4 kb.
Tate
S S
SS
Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey 07110.
Urade
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R
Getchell
T V
TV
Udenfriend
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S
D026905:Amino Acid Transport Systems; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D005796:Genes; D007668:Kidney / Q000378:metabolism*; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008297:Male; D008845:Microinjections; D009865:Oocytes / Q000378:metabolism*; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000494:pharmacology; D014982:Xenopus laevis
D026905:Amino Acid Transport Systems; D000596:Amino Acids; D002352:Carrier Proteins; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D012964:Sodium; D007930:Leucine