Antigenic sites of the muscle protein, paramyosin, in Caenorhabditis elegans determined with exon-expression plasmid.
Sonicated and restricted DNA fragments from plasmid clones containing the paramyosin gene, unc-15 in C. elegans were recloned into lac-z expression plasmid vectors. Numerous fragments were recovered which encoded paramyosin epitopes. These clones produced fusion protein which cross-reacted with a polyclonal and three monoclonal antibodies. The samples of sonicated fragments were also used for shot gun sequencing of the gene. Sequenced fragments were aligned with a computer analysis. Amino acid sequence of the expressed clones matched exactly to that of the exon.
Kagawa
H
H
Department of Biology, Faculty of Science, Okayama University, Japan.
Gengyo
K
K
D000818:Animals; D002107:Caenorhabditis / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D000939:Epitopes / Q000032:analysis / Q000235:genetics*; D005091:Exons; D005796:Genes; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000276:immunology; D014335:Tropomyosin / Q000235:genetics* / Q000276:immunology
D000939:Epitopes; D011993:Recombinant Fusion Proteins; D014335:Tropomyosin