Regulation of the heat-shock response by interferon in mouse L cells.
Interferon (IFN) is not able to induce heat-shock protein (HSP) synthesis. However IFN pretreatment of mouse L cells has been shown to enhance the decrease of overall protein synthesis which follows a heat shock, and to stimulate the accumulation of HSPs. We show here that the synthesis of a protein (the hepatitis B virus surface antigen) under the control of a Drosophila HSP 70 promoter is also stimulated in IFN-pretreated cells. The regulation by IFN takes place at two levels: first, the rate of HSP gene transcription is increased in nuclei isolated from IFN-treated cells; second, the synthesis of HSPs is prolonged after pretreatment with IFN. Experiments performed in the presence of actinomycin D show that this effect is due to a stabilization by IFN of mRNAs coding for HSPs.
Dubois
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Unité de Recherches sur les Infections Virales, INSERM U 43, Hôpital St. Vincent de Paul, France.
Mezger
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V
Morange
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Ferrieux
C
C
Lebon
P
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Bensaude
O
O
D000818:Animals; D015152:Blotting, Northern; D003609:Dactinomycin / Q000494:pharmacology; D004330:Drosophila; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation / Q000187:drug effects*; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006514:Hepatitis B Surface Antigens / Q000235:genetics; D006358:Hot Temperature; D007372:Interferons / Q000494:pharmacology*; D007700:Kinetics; D007739:L Cells; D051379:Mice; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection
D006360:Heat-Shock Proteins; D006514:Hepatitis B Surface Antigens; D012333:RNA, Messenger; D003609:Dactinomycin; D012313:RNA; D007372:Interferons