title: Heat stress in the presence of low RNA polymerase activity increases chromosome copy number of Escherichia coli.
abstract: A temperature shift-up accompanied by a reduction in RNA polymerase activity in Escherichia coli causes an increased rate of initiation leading to a 1.7- to 2.2-fold increase in chromosome copy number. A temperature shift-up without a reduction in polymerase activity induces only a transient non-scheduled initiation of chromosome replication caused by heat shock with no detectable effect on chromosome copy number.
authors: Departamento de Bioquimica y Biologia Molecular y Genetica, Universidad de Extremadura, Badajoz, Spain.
authors: E C
authors: EC
authors: Guzman
authors: A
authors: A
authors: Jimenez-Sanchez
authors: E
authors: E
authors: Orr
authors: R H
authors: RH
authors: Pritchard
mesh_terms: D002876:Chromosomes, Bacterial / Q000502:physiology*; D004269:DNA, Bacterial / Q000096:biosynthesis; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D006358:Hot Temperature; D007700:Kinetics; D010957:Plasmids; D012329:RNA, Bacterial / Q000096:biosynthesis; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012321:DNA-Directed RNA Polymerases