Effect of IL-2 on suppressor factor production.
Suppressor T-cell hybridomas specific for the synthetic polypeptide antigen L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT) release TsF spontaneously and are not dependent on exogenous sources of lymphokines for their growth. IL-2 has no effect on the cell growth of these hybridomas and little overall effect is observed on protein biosynthesis. Nevertheless, the addition of IL-2 to one of these hybridomas (762 B3.7), leads to a substantial increase in suppressor factor (TsF2) production as measured by both bioactivity and direct analysis of 35S-methionine incorporation into TsF2. Treatment of the TsF2 producing hybridoma with phorbol myristate acetate (PMA) causes an increase in the level of IL-2 receptor expression in this hybridoma and enhances the effects of IL-2 on the biosynthesis of TsF2. These data suggest that in addition to its growth promoting properties, IL-2 may provide a signal that triggers suppressor cells to produce antigen specific suppressor factors.
Freire-Moar
J
J
Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110.
Kapp
J A
JA
Webb
D R
DR
D001345:Autoradiography; D001681:Biological Assay; D002455:Cell Division / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D006801:Humans; D006825:Hybridomas / Q000187:drug effects*; D007376:Interleukin-2 / Q000494:pharmacology*; D012313:RNA / Q000096:biosynthesis; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis; D013491:Suppressor Factors, Immunologic / Q000096:biosynthesis*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D013491:Suppressor Factors, Immunologic; D012313:RNA; D013755:Tetradecanoylphorbol Acetate