An improved micro-incubation well for immunocytochemical staining procedures.
A shallow "micro-incubation well" was made with a piece of the square plastic slide and a glass slide. A circular hole (10-12 mm in diameter) was cut out of the center of the square plastic slide. This plastic slide was then attached to the glass slide with a drop of Epon-Araldite and cured in the 60 degrees C oven for 2 days. One to three small sections of tissue mounted within corresponding circles on an ordinary microscopic slide could be stained when placed upside-down on the well in a moisture chamber. The moisture chamber was modified by a disposable square Petri dish (100 x 15 mm) containing a small amount of water and a shallow slide stage which was made by cutting a half inch rim from the bottom of a round disposable Petri dish (100 x 15 mm). This method provided the following advantages in immunocytochemical staining: (1) an even distribution of micro-amounts of antibody solution for long periods of time, and (2) greatly reduced the cost of the immunostaining technique by using micro-amounts of antibody solution.
Yu
S M
SM
Tseng
T M
TM
D000818:Animals; D007150:Immunohistochemistry / Q000379:methods*; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling