Prostate-specific antigenic domain of human prostate specific antigen identified with monoclonal antibodies.
With the use of five murine monoclonal antibodies (1A5, 2A4, 3F1, F5 and 3A12) and an antigen-affinity purified goat polyclonal IgG antibody, the presence of a prostate-specific antigenic domain in human prostate-specific antigen molecule was identified. The results were based upon a series of quantitative competitive inhibition assays of each 125I-labeled monoclonal antibody and polyclonal antibody binding to prostate-specific antigen by unlabeled monoclonal antibodies as inhibitors, and immunohistochemical examination of an extensive panel of human tissue specimens. A cluster of two epitopes that are spatially related or in close topographical proximity and represent a prostate-specific antigenic domain are defined by the monoclonal antibodies 1A5, 2A4, 3F1, and F5, 3A12, respectively.
Chu
T M
TM
Department of Diagnostic Immunology Research, Roswell Park Memorial Institute, Buffalo, NY 14263.
Kawinski
E
E
Hibi
N
N
Croghan
G
G
Wiley
J
J
Killian
C S
CS
Corral
D
D
D000818:Animals; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis*; D001666:Binding Sites, Antibody; D000939:Epitopes / Q000032:analysis*; D006041:Goats / Q000276:immunology; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011467:Prostate / Q000276:immunology*; D017430:Prostate-Specific Antigen; D011471:Prostatic Neoplasms / Q000276:immunology*; D011487:Protein Conformation
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000939:Epitopes; D017430:Prostate-Specific Antigen