[Clonal response of murine myelomonocytic leukemia cells (WEHI-3B-Y1) to purified recombinant human hemopoietic factors in serum-free culture].
We tested purified recombinant hemopoietic factors for their effects on the proliferation and differentiation of murine myelomonocytic leukemia cells (WEHI-3B-Y1) in serum-free agar culture. We found that purified recombinant human G colony-stimulating factor (CSF) markedly increased the colony number of WEHI-3B-Y1 cells and differentiation-inducing activity. However, at low colony densities [( 100/dish), G-CSF did not induce the differentiation of WEHI-3B-Y1 cells. We conclude that G-CSF does not induce the differentiation of WEHI-3B-Y1 cells directly, but induce the differentiation as a result of the secondary autoinduction of differentiation. Interleukin 3 (IL-3) slightly enhanced but erythropoietin (Epo) did not alter the colony number of WEHI-3B-Y1 cells. GM-CSF or M-CSF decreased the colony number of WEHI-3B-Y1 cells. Such purified recombinant human IL-3, Epo, GM-CSF and M-CSF did not induce morphologically the distinct differentiation of WEHI-3B-Y1 cells.
Kajigaya
Y
Y
Sasaki
H
H
Ikuta
K
K
Matsuyama
S
S
D000818:Animals; D001769:Blood; D002454:Cell Differentiation; D002455:Cell Division; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D003470:Culture Media; D016179:Granulocyte Colony-Stimulating Factor; D006801:Humans; D015479:Leukemia, Myelomonocytic, Acute / Q000473:pathology*; D051379:Mice; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000473:pathology; D014410:Tumor Stem Cell Assay
D003115:Colony-Stimulating Factors; D003470:Culture Media; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor