Simultaneous measurements of ionic currents, tension and optical properties of voltage clamped skeletal muscle fibres.
The paper presents the voltage clamp method for isolated skeletal muscle cells, which allows simultaneous measurements of ionic currents, tension and changes in their optical properties. Experimental results illustrate the range of possible applications. Measurements with two types of preparations, frog and crayfish, exhibiting differences in EC coupling are compared. Basic characteristics of the voltage dependence of tension and birefringence during activation of contraction are described.
Poledna
J
J
Centre of Physiological Sciences, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Lacinová
L
L
D000818:Animals; D003400:Astacoidea; D001718:Birefringence; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods; D066298:In Vitro Techniques; D007473:Ion Channels / Q000502:physiology; D008564:Membrane Potentials; D009119:Muscle Contraction; D009132:Muscles / Q000502:physiology*; D011896:Rana temporaria
D007473:Ion Channels