title: A large proportion of T lymphocytes lack CD5 expression after bone marrow transplantation.
abstract: The lymphocyte cell surface molecule CD5 (T1, Leu 1, Tp67 in the human; Ly 1 in the mouse) is expressed on the majority of circulating T lymphocytes and a small population of B cells. We have analyzed CD5 expression on repopulating T cells in the peripheral blood of patients after allogeneic bone marrow transplantation (BMT). The frequency of CD3+ T cells that lack expression of CD5 is dramatically increased after BMT compared with the normal population. The percent of total CD3+ CD5- cells correlated with the presence of graft versus host disease and with time following transplant, but did not correlate with age, diagnosis, preparative regimen, T-cell depletion of the marrow, major histocompatibility complex compatibility, or the presence or absence of interstitial pneumonitis. Furthermore, the total number and percent of CD8+ CD5- cells was increased following BMT. CD3+ cells from BMT patients were sorted for the presence or absence of CD5 expression. CD3+ CD5- cells were capable of interleukin-2 production and of mediating cytolysis following lectin stimulation. We conclude that CD3+ CD5- T cells are functional and represent a significant proportion of circulating cells in patients after BMT.
authors: Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115.
authors: B E
authors: BE
authors: Bierer
authors: S J
authors: SJ
authors: Burakoff
authors: B R
authors: BR
authors: Smith
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000032:analysis* / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte; D016026:Bone Marrow Transplantation; D017252:CD3 Complex; D018956:CD5 Antigens; D002118:Calcium / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D003602:Cytotoxicity, Immunologic; D006086:Graft vs Host Disease / Q000276:immunology; D006801:Humans; D007153:Immunologic Deficiency Syndromes / Q000276:immunology*; D007376:Interleukin-2 / Q000096:biosynthesis; D007958:Leukocyte Count; D051379:Mice; D010641:Phenotype; D011948:Receptors, Antigen, T-Cell; D013602:T-Lymphocytes, Cytotoxic / Q000145:classification* / Q000276:immunology / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D018956:CD5 Antigens; D007376:Interleukin-2; D011948:Receptors, Antigen, T-Cell; D002118:Calcium