A simple technique for sex chromatin analysis in amniotic cells of mouse and rat embryos.
A simple technique has been developed for sex chromatin analysis in the amniotic membrane of rodent embryos. This technique combines the use of 60% acetic acid as a fixative and a carbol fuchsin stain. This technique may be useful for quick sex diagnosis of rodent embryos at midgestation for various purposes in experimental embryology.
Watanabe
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T
Department of Hygiene and Preventive Medicine, Yamagata University School of Medicine, Japan.
Endo
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D000085:Acetates; D019342:Acetic Acid; D000650:Amnion / Q000166:cytology*; D000818:Animals; D005404:Fixatives; D005865:Gestational Age; D051379:Mice; D008813:Mice, Inbred ICR; D051381:Rats; D012394:Rosaniline Dyes; D012728:Sex Chromatin / Q000032:analysis*; D012732:Sex Determination Analysis; D013194:Staining and Labeling / Q000379:methods*
D000085:Acetates; D005404:Fixatives; D012394:Rosaniline Dyes; D019342:Acetic Acid