[Epitope analysis of mumps virus proteins in a persistent cell culture infection: changes in the hemagglutinin-neuraminidase polypeptide].
Monoclonal antibodies (MABs) against major structural mumps virus proteins were used for epitope analysis in primarily and persistently infected HEp-2 cells by means of immunofluorescence and radioimmunoprecipitation techniques. Qualitatively, no differences were found in MAB binding between corresponding proteins of the original and persistent viruses, whereas quantitative differences observed might be explained in terms of weakened viral protein synthesis in persistent infection. Limited proteolysis of MAB-bound antigen has revealed alterations in certain epitopes on persistent virus HN polypeptide. Despite the inability of HEp-2 infected cells for hemadsorption, HN protein was expressed on the surface of these cells to the same extent as in the hemadsorbing system of mumps virus-infected Vero cells.
Boriskin
Iu S
IuS
Bogomolova
N N
NN
Orvell
C
C
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006382:Hemadsorption; D006389:Hemagglutinins, Viral / Q000032:analysis*; D006801:Humans; D009107:Mumps / Q000276:immunology*; D009109:Mumps virus / Q000276:immunology*; D009439:Neuraminidase / Q000032:analysis*; D010455:Peptides / Q000032:analysis*; D011233:Precipitin Tests; D014764:Viral Proteins / Q000032:analysis*; D014776:Virus Cultivation
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006389:Hemagglutinins, Viral; D010455:Peptides; D014764:Viral Proteins; D009439:Neuraminidase
Epitopnyĭ analiz belkov virusa parotita pri persistentnoĭ infektsii kletochnykh kul'tur: izmeneniia v polipeptide gemaggliutinin-neĭraminidaza.