title: Comparison of inhibitory activities of various antiretroviral agents against particle-derived and recombinant human immunodeficiency virus type 1 reverse transcriptases.
abstract: Several known antiretroviral agents were tested for their ability to inhibit the activities of human immunodeficiency virus type 1 reverse transcriptase purified from virions or from a recombinant Escherichia coli strain. The recombinant reverse transcriptase, a polypeptide of 66 kilodaltons, showed inhibition profiles indistinguishable from those of the virion-derived enzyme with all tested compounds, except for suramin and two dextran sulfates. These were more inhibitory to the recombinant enzyme, presumably because the E. coli-derived enzyme was more highly purified. The relative ease with which large quantities of recombinant enzyme can be prepared should facilitate the large-scale screening and identification of new potential inhibitors of the human immunodeficiency virus type 1 reverse transcriptase.
authors: Veterans Administration Medical Center, Atlanta, Georgia.
authors: R F
authors: RF
authors: Schinazi
authors: B F
authors: BF
authors: Eriksson
authors: S H
authors: SH
authors: Hughes
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D015497:HIV-1 / Q000201:enzymology*; D011994:Recombinant Proteins; D018894:Reverse Transcriptase Inhibitors
chemical_list: D000998:Antiviral Agents; D011994:Recombinant Proteins; D018894:Reverse Transcriptase Inhibitors