Measurement of human G-CSF by enzyme-linked immunosorbent assay using monoclonal antibody.
An IgG monoclonal antibody to recombinant human granulocyte colony-stimulating factor (G-CSF), designated HG1, was produced by fusion of immune mouse splenocytes with HAT-sensitive murine myeloma cells. This HG1 was capable of neutralizing the colony-stimulating activity of G-CSF in vitro, and it did not cross-react with human granulocyte/macrophage colony-stimulating factor (GM-CSF). An enzyme-linked immunosorbent assay (ELISA) for measurement of G-CSF was developed using HG1 and a polyclonal antibody against G-CSF raised in a rabbit. The data indicated that the ELISA was highly efficient and sensitive for the detection of as little as 50 pg/ml of recombinant G-CSF. This assay system therefore warrants further attention.
Omori
F
F
Cancer Center, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Okamura
S
S
Takaku
F
F
Niho
Y
Y
D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D016179:Granulocyte Colony-Stimulating Factor; D006801:Humans; D009500:Neutralization Tests
D000911:Antibodies, Monoclonal; D003115:Colony-Stimulating Factors; D016179:Granulocyte Colony-Stimulating Factor