Structures of the sugar chains of recombinant human granulocyte-colony-stimulating factor produced by Chinese hamster ovary cells.
Recombinant human granulocyte-colony-stimulating factor (G-CSF) was purified from Chinese hamster ovary cells transfected with human G-CSF cDNA. The recombinant human G-CSF was treated with alkaline borohydride and the oligosaccharide-alditols liberated were fractioned by gel filtration on a Bio-Gel P-4 column, followed by high-performance liquid chromatography by use of a strong anion exchanger. Two oligosaccharide-alditols were obtained and their structures were identified by component analysis and 500-MHz 1H-NMR spectroscopy. The structures of the sugar chains were NeuAc alpha 2-3Gal beta 1-3GalNAcol and NeuAc alpha 2-3Gal beta 1-3(NeuAc alpha 2-6)GalNAcol.
Oheda
M
M
Department of Chemistry, Osaka University College of Science.
Hase
S
S
Ono
M
M
Ikenaka
T
T
D000818:Animals; D002241:Carbohydrates / Q000032:analysis*; D005591:Chemical Fractionation; D003115:Colony-Stimulating Factors / Q000032:analysis*; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor; D006098:Granulocytes / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000302:isolation & purification; D010053:Ovary / Q000166:cytology; D011994:Recombinant Proteins / Q000032:analysis
D002241:Carbohydrates; D003115:Colony-Stimulating Factors; D009844:Oligosaccharides; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor