title: Influence of aging on the histamine release and membrane fluidity of rat peritoneal mast cells.
abstract: The maturational changes in the degree of homologous passive cutaneous anaphylaxis (PCA) and the histamine release from peritoneal mast cells induced by several secretagogues were studied using Wistar rats (4-40 weeks old). Although the increase in vascular permeability of the rat skin induced by intradermal injection of histamine did not change significantly from one maturation period to the next, 6- to 8-weeks old rats were both the most susceptible to PCA reactions and the most responsive to histamine-releasing stimuli. Among rats in this age group (6-8 weeks), the fluidity of the resting cell membrane and the extent of membrane fluidity increase in response to compound 48/80 were greatest. Analysis of the lipid composition of mast cells indicated that the ratio of cholesterol to phospholipids was lowest at the age of 6-8 weeks. From the present study, we concluded that the maturational changes in the extent of histamine release seem to be related to membrane fluidity, which has a profile similar to that of maturation.
authors: Department of Pharmacology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
authors: M
authors: M
authors: Mio
authors: H
authors: H
authors: Akahori
authors: Y
authors: Y
authors: Sugimoto
authors: M
authors: M
authors: Akagi
authors: K
authors: K
authors: Tasaka
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D004396:Coloring Agents; D006632:Histamine / Q000494:pharmacology; D006636:Histamine Release; D007271:Injections, Intradermal; D050356:Lipid Metabolism; D008297:Male; D008407:Mast Cells / Q000502:physiology*; D008560:Membrane Fluidity / Q000187:drug effects*; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
chemical_list: D004396:Coloring Agents; D010743:Phospholipids; D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine; D002784:Cholesterol