Effect of gamma-aminobutyric acid (GABA) on vasodilation in resistance-sized arteries isolated from the monkey, rabbit, and rat.
The effect of gamma-aminobutyric acid (GABA) and papaverine on cerebral arteries of rat, rabbit, and monkey and the small mesenteric arteries of the rat were studied in vitro with a microvessel apparatus. GABA (1 x 10(-7) to 1 x 10(-3) M) did not affect the basal tension of arteries of rats at rest. In PGF2 alpha-contracted monkey basilar artery and middle cerebral artery and rat basilar artery, cumulative addition of GABA (1 x 10(-7) to 1 x 10(-3) M) did not produce any relaxation. Also in K+-contracted rat basilar artery and small mesenteric artery, cumulative additions of GABA, muscimol, or bicuculline did not result in relaxation. In K+-contracted rabbit basilar artery, GABA did not produce relaxation. However, the addition of papaverine (1 x 10(-7) to 1 x 10(-4) M) in either PGF2 alpha- or K+-contracted arteries, produced a concentration-dependent relaxation in all arteries tested. These results suggest that the failure of GABA or muscimol to induce relaxation is not due to a defect of the arterial smooth muscle relaxant mechanism, but rather is due to the inability of GABA or muscimol to directly relax the artery in this in vitro preparation. Therefore, the hypotensive effect of GABA seen in the rat is probably not due to direct vasodilation of mesenteric or cerebral arteries. These findings lend further support to the idea that GABA mediates its hypotensive effect through its action as an inhibitory neurotransmitter, as previously suggested by others.(ABSTRACT TRUNCATED AT 250 WORDS)
Lai
F M
FM
Department of Cardiovascular Research, American Cyanamid Company, Pearl River, New York.
Tanikella
T
T
Cervoni
P
P
D000818:Animals; D001158:Arteries / Q000187:drug effects; D001640:Bicuculline / Q000494:pharmacology; D015237:Dinoprost / Q000494:pharmacology; D066298:In Vitro Techniques; D008252:Macaca fascicularis; D008297:Male; D009118:Muscimol / Q000494:pharmacology; D009126:Muscle Relaxation / Q000187:drug effects; D010208:Papaverine / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D014655:Vascular Resistance / Q000187:drug effects; D014664:Vasodilation / Q000187:drug effects*; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
D009118:Muscimol; D005680:gamma-Aminobutyric Acid; D011189:Potassium Chloride; D015237:Dinoprost; D010208:Papaverine; D011188:Potassium; D001640:Bicuculline