title: Heterogeneity of the changes in cytoplasmic pH upon serum stimulation of quiescent fibroblasts.
abstract: Addition of mitogens to quiescent cells results in rapid ionic changes in the cytoplasm, including pH. We studied the changes in cytoplasmic pH in single Swiss 3T3 cells upon serum stimulation using fluorescence ratio imaging microscopy. Quiescence was attained using two approaches, serum deprivation of subconfluent cells and confluence. All measurements were made in the presence of bicarbonate and the absence of other organic buffers. We also used BCECF coupled to dextran to avoid several artifacts associated with using BCECF-AM, including leakage and phototoxicity. Analysis of the changes in cytoplasmic pH demonstrated a dramatic heterogeneity in the responses of single cells. There were six basic classes of responses, 1) a fast alkalinization, reaching a maximum pH in approximately 2-5 min; 2) a slow alkalinization, reaching a maximum pH in 10-20 min; 3) a very slow alkalinization, not reaching a plateau pH within the measurement time; 4) no apparent change in pH during the measurement time; 5) an early transient acidification, followed by either a fast or slow alkalinization; and 6) an acidification, followed by alkalinization and then by a decrease to some intermediate pH. Subconfluent cells exhibited greater heterogeneity in response than confluent cells, with no single dominant class of response. The dominant (55%) response for confluent cells was a gradual alkalinization of approximately 0.01 pH units/min. A larger proportion (52%) of subconfluent cells exhibited an early transient acidification compared to confluent cells (7%). A significant proportion of both types of cells (23% subconfluent, 36% confluent) exhibited no change in cytoplasmic pH upon stimulation. In general, the kinetics of changes in cytoplasmic pH were significantly different from the published results with population averaging methods.
authors: Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213.
authors: G R
authors: GR
authors: Bright
authors: J E
authors: JE
authors: Whitaker
authors: R P
authors: RP
authors: Haugland
authors: D L
authors: DL
authors: Taylor
mesh_terms: D000818:Animals; D003470:Culture Media / Q000494:pharmacology; D003593:Cytoplasm / Q000032:analysis / Q000502:physiology*; D003911:Dextrans; D005347:Fibroblasts / Q000187:drug effects* / Q000502:physiology; D005452:Fluoresceins; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron
chemical_list: D003470:Culture Media; D003911:Dextrans; D005452:Fluoresceins; C043829:2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein