A simplified high speed multicolor immunoblotting method.
We describe a simplified technique for the rapid and specific detection of antigenic determinants within a mixed population of antigens. Each determinant develops its own characteristic color in a single Western blot or dot blot. This multicolor immunostaining technique can be achieved with as little as a one-step incubation, involving a mixture of different primary and developing antibodies, and a one-step substrate reaction, involving a mixture of different substrates. The time required can be reduced to short periods of time, ranging from minutes to about 1 h. This can represent an increase in the speed of detection by one to two orders of magnitude when compared with conventional methods. The simplified protocols may facilitate the automation of routine analyses.
Lee
N
N
Molecular Genetics Department, Interferon Sciences, Inc., New Brunswick, New Jersey 08901.
Yang
J S
JS
Testa
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D
D000906:Antibodies; D000941:Antigens / Q000032:analysis; D015153:Blotting, Western / Q000379:methods; D015151:Immunoblotting / Q000379:methods*; D013194:Staining and Labeling
D000906:Antibodies; D000941:Antigens