Selective detachment of a tetraploid subline of primary keratinocytes after treatment with the tumor promoter 12-0-tetradecanoylphorbol-13-acetate.
TPA treatment of primary keratinocytes in medium with either 0.02 mM Ca++ or 1.2 mM Ca++ enhances terminal differentiation assessed by the production of cornified cells. A tetraploid keratinocyte stemline which appears soon after establishing primary cultures is selectively detached from the culture dish after treatment with the tumor promoter TPA. The remaining cells proliferate in the presence of TPA. This selective effect of TPA on subpopulations of keratinocytes may be critical to its activity as a tumor promoter.
Clausen
O P
OP
Institute of Pathology, Rikshospitalet, University of Oslo, Norway.
Kirkhus
B
B
Elgjo
K M
KM
Yuspa
S H
SH
Kilkenny
A E
AE
Hennings
H
H
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D000078404:Epidermal Cells; D005434:Flow Cytometry; D007633:Keratins; D051379:Mice; D011123:Polyploidy; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D007633:Keratins; D004247:DNA; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium