Transduction of proto-src sequences in tissue culture by a molecular clone of transformation-defective Rous sarcoma virus with an internal src deletion.
The sporadic appearance of nondefective (nd) Rous sarcoma virus (RSV) from cells in tissue culture infected with a molecular clone of transformation-defective RSV was examined. Southern analysis of extrachromosomal, virus-specific DNA of three independent ndRSV isolates in each case indicated restoration of an isogenic src by homologous recombination with cellular proto-src. The frequency of transduction was estimated by fluctuation analysis to vary between one transduction per 0.4 x 10(7) to 1.6 x 10(7) infected cells.
Phares
W
W
Department of Molecular Biology, University of California, Berkeley 94720.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D015139:Blotting, Southern; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002642:Chick Embryo; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis; D003673:Defective Viruses / Q000235:genetics*; D005347:Fibroblasts; D009154:Mutation; D015689:Oncogene Protein p21(ras); D009856:Oncogene Proteins, Viral / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016392:Proto-Oncogene Proteins pp60(c-src); D011995:Recombination, Genetic; D014161:Transduction, Genetic
D004279:DNA, Viral; D009856:Oncogene Proteins, Viral; D011518:Proto-Oncogene Proteins; D016392:Proto-Oncogene Proteins pp60(c-src); D004262:DNA Restriction Enzymes; D015689:Oncogene Protein p21(ras)