[Study on karyotypes C-banding and Ag-NORs of Rana minimus and Hyla chinensis from Fuzhou].
Using bone marrow preparing chromosome method, BSG technique and a quick Ag-stained NORs method, the conventional, C-banded and Ag-stained karyotypes of Rana minimus Ting et Ts'ai and Hyla chinensis Guenther were studied. The diploid number of R. minimus is 2n = 26, consisting of 5 pairs of large and 8 pairs of small chromosomes. A secondary constriction is observed at a position near the centromere on the short arm of chromosome No. 6 and it is also the position of the "standard NORs" Chromosome C-banding shows that the heterochromations of R. minimus are located in the centromeric and pericentromeric regions, with comparatively few lightly-stained interstitial C-bands and no telomeric ones. And the secondary constriction is C-banding positively stained. H. chinensis has 24 chromosomes which divide into a group of 6 large pairs and a group of 6 small pairs. A secondary constriction appears at a position on the long arm of chromosome No. 10. It is also the position of the "standard NORs" and C-banding positively stained. The C-banding pattern is mainly composed of centromeric C-bands with few interstitial ones and no telomeric ones. The karyotypic characters of Ranidae and Hylidae and the relationship to the evolution are also discussed.
Gao
J M
JM
D000818:Animals; D001001:Anura / Q000235:genetics*; D002681:China; D002871:Chromosome Banding; D005260:Female; D007621:Karyotyping; D008297:Male; D009697:Nucleolus Organizer Region; D011898:Ranidae / Q000235:genetics*; D012834:Silver; D013194:Staining and Labeling
D012834:Silver