The effects of TGF beta on haemopoietic cells.
Transforming growth factor beta (TGF beta) suppresses the growth of differentiation inducible, murine IL-3-dependent multipotential cell lines but has no growth inhibitory effect upon an IL-3-independent (leukaemic) cell line arising from one of them, nor on IL-3-dependent cell lines that are unable to undergo differentiation. TGF beta inhibits in vitro colony formation by normal multipotential haemopoietic progenitor cells. Bipotential progenitors recruited by GM-CSF are, however, more resistant to the inhibitory effects of TGF beta, whereas progenitors recruited by the lineage restricted factor, M-CSF, are sensitive to the inhibitory effects. These data indicate that responsiveness to TGF beta is differentiation linked and studies with the cell lines suggest that response (or lack of response is not determined solely by levels of expression of TGF beta receptors. Furthermore, the effects of TGF beta 2 on haemopoietic progenitors are very similar to those induced by TGF beta.
Hampson
J
J
Department of Experimental Haematology, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Withington, Manchester, United Kingdom.
Ponting
I L
IL
Cook
N
N
Vodinelich
L
L
Redmond
S
S
Roberts
A B
AB
Dexter
T M
TM
D000818:Animals; D001854:Bone Marrow Cells; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D002999:Clone Cells; D003115:Colony-Stimulating Factors / Q000502:physiology; D005260:Female; D005434:Flow Cytometry; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006098:Granulocytes / Q000502:physiology; D006133:Growth Substances / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D007377:Interleukin-3 / Q000502:physiology; D016173:Macrophage Colony-Stimulating Factor; D008264:Macrophages / Q000502:physiology; D051379:Mice; D011994:Recombinant Proteins / Q000494:pharmacology; D015291:Transforming Growth Factors / Q000502:physiology*
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D007377:Interleukin-3; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D015291:Transforming Growth Factors; D016173:Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor