DNA curvature in front of the human mitochondrial L-strand replication origin with specific protein binding.
DNA bending has been suggested to play a role in the regulation of gene expression, initiation of DNA-replication, site specific recombination, and DNA packaging. In the human mitochondrial DNA we have found a DNA curvature structure within the 3'-region of ther URF2 sequence in front of the L-strand origin of replication. This structure interacts specifically with a protein factor isolated from mitochondria. Based on the localization of this DNA curvature structure and the known function of such structures the data suggest a model in which this DNA signal sequence and its specific protein binding is involved in the regulatory initiation event of L-strand replication.
Welter
C
C
Institute of Human Genetics, Medical School, University of Saar, FRG.
Dooley
S
S
Zang
K D
KD
Blin
N
N
D001483:Base Sequence; D004261:DNA Replication; D004272:DNA, Mitochondrial; D004268:DNA-Binding Proteins / Q000378:metabolism; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D009690:Nucleic Acid Conformation; D015183:Restriction Mapping
D004272:DNA, Mitochondrial; D004268:DNA-Binding Proteins; D015245:Deoxyribonuclease BamHI; D015246:Deoxyribonuclease EcoRI; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific