Routine diagnostic immunohistochemical labeling of extracellular antigens in formol saline solution-fixed, paraffin-embedded cutaneous tissue.
A technique involving modification of the routine paraffin embedding procedure that allows immunohistochemical examination of extracellular antigens by light microscopy is described. The method is thus suitable for routine diagnostic immunofluorescence studies, permitting reliable, reproducible immunolabeling of immunoglobulins and complement components usually not preserved by routine processing procedures. Immunoreactivity as measured by direct immunofluorescence and immunoenzymatic (peroxidase-antiperoxidase) histochemistry is comparable to results with fresh-frozen cryostat sections. Morphologic preservation is superior, however, and the processed material is suitable for routine hematoxylin-eosin staining.
Cerio
R
R
Laboratory of Applied Dermatopathology, Guy's Hospital, London, England.
MacDonald
D M
DM
D000954:Antigens, Surface / Q000032:analysis; D003165:Complement System Proteins / Q000032:analysis; D005404:Fixatives; D005455:Fluorescent Antibody Technique; D005557:Formaldehyde; D006652:Histological Techniques; D006801:Humans; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins / Q000032:analysis; D007150:Immunohistochemistry; D010232:Paraffin; D012871:Skin Diseases / Q000175:diagnosis* / Q000473:pathology; D012965:Sodium Chloride; D013194:Staining and Labeling
D000954:Antigens, Surface; D005404:Fixatives; D007136:Immunoglobulins; D005557:Formaldehyde; D012965:Sodium Chloride; D010232:Paraffin; D003165:Complement System Proteins