title: Anti-tubulin monoclonal antibodies that bind to specific neurons in Caenorhabditis elegans.
abstract: We have identified 2 anti-tubulin monoclonal antibodies that bind to 2 different subpopulations of identified neurons in the simple nervous system of the nematode Caenorhabditis elegans. The antibodies also recognize specific tubulin isotypes from C. elegans that were separated by isoelectric focusing. Antibody 6-1 1B-1 intensely stained the mechano-sensory neurons ALML, ALMR, PLML, PLMR, PVM, and AVM, plus neuron PVR by indirect immunofluorescence, and it bound to 1 of 2 major alpha-tubulin isotypes separated by iso-electric focusing. The epitope for antibody (Ab) 6-1 1B-1 is acetylated alpha-tubulin (Piperno and Fuller, 1985). Antibody 2-28-33 stained a set of neurons that contain the neurotransmitter GABA. It bound to the 2 major and 1 of the minor beta-tubulin isotypes of C. elegans. These results suggest that specific alpha- and beta-tubulin isotypes are greatly enriched in the subsets of neurons recognized by these antibodies. Most (possibly all) of the neurons in each subset are functionally related. The antibodies should allow us to examine whether specific tubulin isotypes have specific functions in these neurons. They may also be useful for examining neuron morphologies in mutants of C. elegans which affect nerve development.
authors: Department of Biochemistry, Molecular and Cell Biology, Northwestern University, Evanston, Illinois 60201.
authors: S S
authors: SS
authors: Siddiqui
authors: E
authors: E
authors: Aamodt
authors: F
authors: F
authors: Rastinejad
authors: J
authors: J
authors: Culotti
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D002107:Caenorhabditis; D007150:Immunohistochemistry; D007525:Isoelectric Focusing; D009474:Neurons / Q000145:classification / Q000276:immunology*; D013194:Staining and Labeling; D014018:Tissue Distribution; D014404:Tubulin / Q000276:immunology* / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D014404:Tubulin