Enhancer-like interferon responsive sequences of the human and murine (2'-5') oligoadenylate synthetase gene promoters.
The human (2'-5') oligo(A) synthetase gene contains two independent cis-acting DNA elements, A and B, which act as transcriptional enhancers. Element A alone is not activated by IFN treatment. Element B alone confers IFN-inducibility to the herpes tk promoter. Two murine (2'-5') oligo(A) synthetase genes were isolated and their promoter sequences show high conservation of element A and B. A synthetic oligonucleotide, containing 16 bp of the human element B, or 14 bp of the homologue murine element B, was linked to a TK-CAT construct. These oligonucleotides were shown to be sufficient to activate the TK promoter in the presence of IFN. When multiple repeats of the interferon-responsive sequence (E-IRS) were cloned in 5' of the TK promoter, the activation ratio was increased. In vitro, specific binding of nuclear protein(s) is observed to the radiolabelled synthetic human E-IRS. This binding is competed by the addition of cold synthetic mouse E-IRS or fragments of genomic DNA containing the E-IRS.
Cohen
B
B
Department of Virology, Weizmann Institute of Science, Rehovot, Israel.
Peretz
D
D
Vaiman
D
D
Benech
P
P
Chebath
J
J
D015088:2',5'-Oligoadenylate Synthetase / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004742:Enhancer Elements, Genetic / Q000187:drug effects*; D006649:Histocompatibility Antigens / Q000235:genetics; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D011485:Protein Binding; D012689:Sequence Homology, Nucleic Acid
D006649:Histocompatibility Antigens; D004247:DNA; D007372:Interferons; D015088:2',5'-Oligoadenylate Synthetase