A simple, sensitive method of analyzing bacterial ribosomal DNA polymorphism.
A rapid DNA extraction procedure and random primer labelling of Escherichia coli ribosomal RNA with cloned reverse transcriptase have been used to establish a simple and highly sensitive method for studying ribosomal DNA polymorphism in bacteria. Examples of inter- and intraspecies differentiation of bacteria are given and potential applications in bacterial epidemiology and taxonomy are discussed.
Picard-Pasquier
N
N
Institut de Pathologie Moléculaire, Institut National de la Santé et de la Recherche Médicale, Paris, France.
Ouagued
M
M
Picard
B
B
Goullet
P
P
Krishnamoorthy
R
R
D000150:Acinetobacter / Q000235:genetics; D001345:Autoradiography; D001419:Bacteria / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis*; D004275:DNA, Ribosomal / Q000032:analysis*; D004755:Enterobacteriaceae / Q000235:genetics; D004996:Ethidium; D008722:Methods; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D015347:RNA Probes; D012194:RNA-Directed DNA Polymerase; D013045:Species Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D015347:RNA Probes; D012194:RNA-Directed DNA Polymerase; D004996:Ethidium