T-lymphocyte clone specific for pancreatic islet antigen.
A cloned T-lymphocyte line, BDC-2.5, was derived from a nonobese diabetic (NOD) mouse and has been found to exhibit specificity for islet cell antigen in vitro and in vivo. This clone is a CD4+ T-lymphocyte that proliferates and makes lymphokine in response to islet cell antigen- and NOD antigen-presenting cells. In an in vivo transplantation system in which islet grafts were made in the presence or absence of the BDC-2.5 T-lymphocytes, it was found that incorporation of the islet-specific T-lymphocytes into the graft site resulted in complete destruction of the transplanted tissue. Similar grafts made with pituitary tissue were not affected by the T-lymphocyte clone. These results suggest that the islet-specific T-lymphocytes mediate islet destruction in a tissue-specific manner.
Haskins
K
K
Barbara Davis Center for Childhood Diabetes, University of Colorado, Health Sciences Center, Denver 80262.
Portas
M
M
Bradley
B
B
Wegmann
D
D
Lafferty
K
K
D000818:Animals; D001324:Autoantigens / Q000276:immunology*; D002460:Cell Line; D002469:Cell Separation; D002999:Clone Cells; D000939:Epitopes / Q000276:immunology*; D007515:Islets of Langerhans / Q000276:immunology*; D016381:Islets of Langerhans Transplantation; D008222:Lymphokines / Q000096:biosynthesis; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*
D001324:Autoantigens; D000939:Epitopes; D008222:Lymphokines