Conformation of gramicidin in relation to its ability to form bilayers with lysophosphatidylcholine.
The ability of gramicidin to induce bilayer formation in lysophosphatidylcholine (LPC) systems was investigated as a function of the conformation of the peptide. The conformation was varied by using different solvents to cosolubilize gramicidin and lipid. Using circular dichroism (CD), it was found that when codissolved in trifluoroethanol (TFE), after drying and subsequent hydration, gramicidin is mainly present in the single-stranded beta 6.3-helical configuration, whereas when using chloroform/methanol or ethanol as the solvent, it is proposed that the dominant conformation of gramicidin in the membrane is that of the double-stranded antiparallel dimer. Employing 31P NMR, the stoichiometry for bilayer formation was found to be 6 to 7 lipid molecules per gramicidin monomer, when samples were prepared from TFE, whereas a stoichiometry of 4 was found when chloroform/methanol or ethanol was the solvent. Upon heating the latter samples, a conversion was observed in the CD pattern toward that indicative of the beta 6.3-helical configuration. This change was accompanied by an increase in the extent of bilayer formation. Next, it was investigated whether the conformation of gramicidin and its ability to induce bilayer formation were dependent on the lipid acyl chain length. CD measurements of samples prepared from TFE indicated that gramicidin, independent of acyl chain length, was present in the beta 6.3-helical configuration but the intensity of the ellipticities at 218 nm increased with the length of the acyl chain. The extent of bilayer formation in these samples was found to be largely chain length independent.(ABSTRACT TRUNCATED AT 250 WORDS)
Killian
J A
JA
Laboratory of Molecular Biophysics, School of Medicine, University of Alabama, Birmingham 35294.
Urry
D W
DW
D002462:Cell Membrane / Q000378:metabolism; D002942:Circular Dichroism; D000431:Ethanol; D006096:Gramicidin / Q000378:metabolism*; D007473:Ion Channels; D008051:Lipid Bilayers / Q000378:metabolism*; D008244:Lysophosphatidylcholines / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D010758:Phosphorus; D011487:Protein Conformation; D012964:Sodium; D013329:Structure-Activity Relationship; D014270:Trifluoroethanol
D007473:Ion Channels; D008051:Lipid Bilayers; D008244:Lysophosphatidylcholines; D006096:Gramicidin; D010758:Phosphorus; D000431:Ethanol; D014270:Trifluoroethanol; D012964:Sodium