title: Biological and cellular responses to grass pollen in sensitive patients.
abstract: Using a noninvasive skin chamber technique, we studied the in vivo development of anaphylactic reactions in 8 pollen-sensitive patients suffering from seasonal allergic rhinitis/conjunctivitis/asthma and showing positive cutaneous reactions after intradermal allergen challenge. As agonists, histamine and pollen were introduced into the skin chambers and left in contact with superficial dermis during 6 h. The release of mediators (histamine and prostaglandin [PG] D2) and the modifications in protein diffusion occurring during the immediate (30 min) and the late (6 and 24 h) cutaneous reaction phases were quantitatively analyzed. 24 h after agonist introduction, the recruitment of inflammatory cells on the superficial dermis was studied by use of Rebuck's windows. Histamine release in pollen-containing skin chambers was immediate and persisted until the 24th h despite replacement of the agonists by control medium at the 6th h. An intense PGD2 release occurred as soon as the first 30 min in chambers containing either exogenous histamine or pollen and was maintained until the 24th h. Protein diffusion induced by histamine and pollen was similar to the control one at 30 min but was intensely enhanced at the 6th h. At the 24th h, pollen-induced protein diffusion was still intense whereas that induced by histamine was analogous to the control one. 24 h after pollen challenge, numerous eosinophils were recruited on the superficial dermis but almost none were observed after control medium or histamine.(ABSTRACT TRUNCATED AT 250 WORDS)
authors: Unité INSERM U 312, Service de Dermatologie, Hospital Henri Mondor, Créteil, France.
authors: L
authors: L
authors: Dubertret
authors: L
authors: L
authors: Michel
authors: F
authors: F
authors: Jean-Louis
authors: Y
authors: Y
authors: Denizot
mesh_terms: D004804:Eosinophils / Q000378:metabolism; D006636:Histamine Release; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology*; D011058:Pollen / Q000276:immunology*; D015230:Prostaglandin D2 / Q000032:analysis / Q000378:metabolism*; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology*; D012867:Skin / Q000276:immunology; D012884:Skin Window Technique; D013997:Time Factors
chemical_list: D015230:Prostaglandin D2