title: A dual transcriptional activation system for the 230 kb plasmid genes coding for virulence-associated antigens of Shigella flexneri.
abstract: The expression of plasmid-encoded, invasion-related antigens lpa b, c and d of Shigella flexneri was found to be positively regulated at transcriptional level by a 33kD protein produced by the previously defined, virulence-associated Region 1 on the SalI fragment B of the 230 kb invasion plasmid. The gene (designated virB) was identified and its nucleotide sequence determined. No Ipa b or c was produced in the absence of an intact virB gene although lower levels of d were produced. The previously reported regulatory activity of the virF gene some 30 kb distance away was shown to act exclusively through virB. In contrast, the activation of the virG gene necessary for intercellular spread occurred directly by virF without the requirement for virB. This study thus ascribes a critical function to a previously recognized, but functionally undefined, virulence locus on the large invasion plasmid of S. flexneri. The virF gene appears to have a central role in activation of the 230 kb plasmid-encoded virulence genes.
authors: Department of Bacteriology, Institute of Medical Science, University of Tokyo, Japan.
authors: B
authors: B
authors: Adler
authors: C
authors: C
authors: Sasakawa
authors: T
authors: T
authors: Tobe
authors: S
authors: S
authors: Makino
authors: K
authors: K
authors: Komatsu
authors: M
authors: M
authors: Yoshikawa
mesh_terms: D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics* / Q000502:physiology; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001482:Base Composition; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D015151:Immunoblotting; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010641:Phenotype; D010957:Plasmids; D012329:RNA, Bacterial / Q000235:genetics; D012763:Shigella flexneri / Q000235:genetics* / Q000472:pathogenicity; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology; D014158:Transcription, Genetic; D037521:Virulence Factors
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D014157:Transcription Factors; D037521:Virulence Factors