title: Na+ currents in cultured mouse pancreatic B-cells.
abstract: Pancreatic B-cells, kept in culture for 1-4 days, were studied in the whole-cell, cell-attached and outside-out modes of the patch clamp technique. B-cells were identified by the appearance of electrical activity in the cell-attached mode when the bath glucose was raised from 3 to 20 mM. In whole-cell, 80% of these cells showed a transient inward Na+ current, when depolarizing pulses were preceded by holding potentials, or prepulses to potentials more negative than -80 mV. The midpoint (Eh) of the inactivation curve (h infinity) was at -109 mV in 2.6 mM Ca2+, 1.2 mM Mg2+ and -120 mV in 0.2 mM Ca2+, 3.6 mM Mg2+. In 2.6 mM Ca2+, inactivation was fully removed at E less than -150 mV. Na+ currents activated at E greater than -60 mV and were largest at around -10 mV (120 mM Na+). The kinetic parameters of activation (tp) and inactivation (tau h) were similar to those of other mammalian Na+ channels. Unitary currents with an amplitude of approximately 1 pA at -30 mV (140 mM Na+) with a similar voltage-dependence and time-course of mean current were recorded from outside-out patches. The results show that B-cells have a voltage-dependent Na+ current which, owing to the voltage-dependence of inactivation, is unlikely to play a major role in glucose-induced electrical activity.
authors: I. Physiologisches Institut der Universität des Saarlandes, Homburg/Saar, Federal Republic of Germany.
authors: T D
authors: TD
authors: Plant
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004594:Electrophysiology; D005947:Glucose / Q000378:metabolism; D007473:Ion Channels / Q000502:physiology*; D007515:Islets of Langerhans / Q000378:metabolism*; D007700:Kinetics; D008433:Mathematics; D051379:Mice; D009994:Osmolar Concentration; D012964:Sodium / Q000378:metabolism*
chemical_list: D007473:Ion Channels; D012964:Sodium; D005947:Glucose