Suppressor activity in the supernatant of T cell clones derived from chimeric mouse spleen cells.
The supernatant from cultures of T cell clones derived from (BALB/c----C3H/He) chimeras suppresses BALB/c anti-C3H/He or BALB/c anti-C57BL/6 MLRs. When we studied the alloantigen specificity of the suppressor activity in culture supernatant, we observed three types of the suppressor activity (i.e., the suppressor activity against BALB/c anti-C3H/He MLR, against BALB/c anti-C57BL/6 MLR, and against both MLRs) on day 3 after stimulation of the T cell clones with 20% crude IL2 and feeder cells. Since the alloantigen specificity fluctuated somewhat with time, we considered that a time-course study was needed to determine it correctly. We thought it unlikely that any IFN-gamma or PGE2 in the culture supernatant of the T cell clones would have mediated the suppression. Our results suggest that alloantigen specific and non-specific suppressor T cells exist in bone marrow chimeras. The former appears to play an important role in inducing and maintaining transplantation tolerance, while the latter seems to have a rather harmful effect upon chimeras.
Imamura
M
M
Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
Fujimoto
H
H
Fukuhara
T
T
Kobayashi
M
M
Hashino
S
S
Kasai
M
M
Sakurada
K
K
Miyazaki
T
T
D000818:Animals; D001853:Bone Marrow / Q000528:radiation effects; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002999:Clone Cells / Q000166:cytology; D003602:Cytotoxicity, Immunologic; D015232:Dinoprostone; D000939:Epitopes; D006086:Graft vs Host Disease / Q000276:immunology; D007108:Immune Tolerance; D051379:Mice; D011458:Prostaglandins E / Q000494:pharmacology; D011828:Radiation Chimera; D013601:T-Lymphocytes / Q000166:cytology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000528:radiation effects; D013997:Time Factors; D014181:Transplantation Immunology
D000939:Epitopes; D011458:Prostaglandins E; D015232:Dinoprostone