title: Metabolic processes involved in repair of Escherichia coli cells damaged by exposure to acid mine water.
abstract: Escherichia coli was stressed by exposure to filter-sterilized acid mine water. Synthetic processes required for repair of sublethally injured survivors were studied by the addition of specific metabolic inhibitors to a resuscitation broth. Inhibitors of protein, RNA, DNA, lipid, and peptidoglycan synthesis as well as uncouplers and inhibitors of electron transport and ATPase activity were used. Acid mine water injury was severe, causing damage to the outer and cytoplasmic membranes. Repair of sublethally injured cells required protein, RNA, and lipid synthesis as well as a proton motive force.
authors: Division of Plant and Soil Sciences, West Virginia University, Morgantown 26506-6057.
authors: A T
authors: AT
authors: Wortman
authors: G K
authors: GK
authors: Bissonnette
mesh_terms: D019297:2,4-Dinitrophenol; D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors; D001426:Bacterial Proteins / Q000096:biosynthesis; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002569:Cerulenin / Q000494:pharmacology; D002701:Chloramphenicol / Q000494:pharmacology; D003523:Cycloserine / Q000494:pharmacology; D004261:DNA Replication / Q000187:drug effects; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D004140:Dinitrophenols / Q000494:pharmacology; D004579:Electron Transport / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D008055:Lipids / Q000096:biosynthesis; D009268:Nalidixic Acid / Q000494:pharmacology; D010457:Peptidoglycan / Q000096:biosynthesis; D011190:Potassium Cyanide / Q000494:pharmacology; D012329:RNA, Bacterial / Q000096:biosynthesis; D012293:Rifampin / Q000494:pharmacology; D014475:Uncoupling Agents / Q000494:pharmacology; D014871:Water Microbiology; D014876:Water Pollution / Q000032:analysis*
chemical_list: D001426:Bacterial Proteins; D004140:Dinitrophenols; D008055:Lipids; D010457:Peptidoglycan; D012329:RNA, Bacterial; D014475:Uncoupling Agents; D002569:Cerulenin; D009268:Nalidixic Acid; D004024:Dicyclohexylcarbodiimide; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D002701:Chloramphenicol; D003523:Cycloserine; D000251:Adenosine Triphosphatases; D011190:Potassium Cyanide; D019297:2,4-Dinitrophenol; D012293:Rifampin