Photodynamic action of rose bengal on isolated rat pancreatic acini: stimulation of amylase release.
The halogenated fluorescein derivative, rose bengal, upon photon activation, elicits amylase secretion from isolated, perifused pancreatic acini. This effect is due to production of highly reactive singlet delta oxygen which can permeabilize the cell membrane and may also react chemically with secretagogue receptors, or other functional components of the membrane such as the G-proteins. The profile of photodynamically induced amylase secretion is anion-dependent: it becomes biphasic when the chloride ion is substituted by the glutamate ion, an effect attributed to the action of glutamate on the ionic transport systems of the zymogen granule membrane.
Matthews
E K
EK
Department of Pharmacology, University of Cambridge, England.
Cui
Z J
ZJ
D000681:Amylases / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D002463:Cell Membrane Permeability; D002712:Chlorides / Q000378:metabolism; D005609:Free Radicals; D019204:GTP-Binding Proteins / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010179:Pancreas / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D010777:Photochemistry; D051381:Rats; D012395:Rose Bengal / Q000494:pharmacology* / Q000528:radiation effects
D002712:Chlorides; D005609:Free Radicals; D005971:Glutamates; D012395:Rose Bengal; D000681:Amylases; D019204:GTP-Binding Proteins; D010100:Oxygen