[Protein malnutrition and response of pancreatic acinar cells to stimulation by cholecystokinin].
Pancreatic lobules were isolated from 2 groups of male Wistar rats after 23 days of diet. A control group (C) fed on a 20% protein diet (16% gluten + 4% casein) and an experimental group (E) on a 5% protein diet (4% gluten + 1% casein). After isolation, lobules were preincubated 10 min with 10 muCi [3H]-leucine, washed, then incubate within Krebs Ringer bicarbonate Hepes. Basal secretion, then stimulated secretion (50 pM of cholecystokinin (CCK] of radioactive and non-radioactive protein and amylase outputs were measured. During basal secretion, in (E) group, lobules secreted more proteins than (C) one, the same outputs of amylase and radioactive protein were observed in both groups. The stimulated secretion by CCK increased the outputs of non-radioactive protein and amylase of lobules (T) (2-3 fold), but was without effect on lobule (E) outputs. Therefore, a low-protein diet involved a decrease of CCK sensibility on acinar cells, this fact might be mediated by a decreasing number and/or affinity of their CCK receptors.
Prost
J
J
Université de Bourgogne, Laboratoire de Physiologie Animale et de la Nutrition, Dijon.
Belleville
J
J
D000681:Amylases / Q000378:metabolism; D000818:Animals; D001699:Biometry; D002766:Cholecystokinin / Q000494:pharmacology*; D008297:Male; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D011502:Protein-Energy Malnutrition / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D011506:Proteins; D002766:Cholecystokinin; D000681:Amylases
Malnutrition protéique et réponse des cellules acineuses pancréatiques à la stimulation par la cholécystokinine.