Adhesiveness of Pseudomonas aeruginosa to rabbit vesical mucosa effect of glycosidases on cellular binding.
The adhesiveness of a mucous strain of Pseudomonas aeruginosa to rabbit bladder mucosa has been investigated after pretreatment of the vesical mucosa with different glycosidases. Using a simple apparatus the study was carried out in parallel on samples of untreated and pre-treated mucosa. The results obtained showed that the cleavage of terminal sugar by addition of neuraminidase, N-acetyl-beta-D-glucosaminidase, alpha-mannosidase, alpha-L-fucosidase and beta-galactosidase produced a decrease in the number of adhered bacteria. A more drastic enzymatic action due to the combined effect of neuraminidase + alpha-L-fucosidase and neuraminidase + beta-galactosidase produced, on the other hand, an increased adhesion of bacteria, probably due to an unmasking of new receptor sites.
Chiarini
F
F
Mastromarino
P
P
Orsi
G B
GB
Riscaldati
T
T
D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects*; D006026:Glycoside Hydrolases / Q000494:pharmacology*; D009092:Mucous Membrane / Q000187:drug effects / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D011817:Rabbits; D011971:Receptors, Immunologic / Q000187:drug effects; D001743:Urinary Bladder / Q000187:drug effects / Q000382:microbiology*
D011971:Receptors, Immunologic; C047105:bacterial adhesin receptor; D006026:Glycoside Hydrolases