Formaldehyde fixation and microwave irradiation.
Formaldehyde is the most commonly used fixative in pathology laboratories. However, due to time pressures, this fixative is often not optimally exploited. The majority of biopsies are only partly fixed when histoprocessing is started, with adverse effects. This paper reports how formaldehyde fixation is improved, by using 1.5 min of microwave irradiation of tissue previously soaked for four hours in the fixation solution. It is argued that this beneficial effect of microwave irradiation can be attributed to the acceleration of the reaction of formaldehyde to the tissue. Formation of free formaldehyde, by the dehydration of methylene glycol present in the tissue when the irradiation starts, is also enhanced. Five different formaldehyde-containing fixatives were evaluated, using five different working protocols. Spleen was taken as a suitable tissue for these tests. The technique described leads to uniform microscopical results. It is a simple method and is suitable for use in routine laboratories.
Boon
M E
ME
Leiden Cytology and Pathology Laboratory, The Netherlands.
Gerrits
P O
PO
Moorlag
H E
HE
Nieuwenhuis
P
P
Kok
L P
LP
D005557:Formaldehyde; D006652:Histological Techniques; D006801:Humans; D007202:Indicators and Reagents; D008872:Microwaves; D013194:Staining and Labeling
D007202:Indicators and Reagents; D005557:Formaldehyde