Competition between splicing and polyadenylation reactions determines which adenovirus region E3 mRNAs are synthesized.
Complex transcription units encode multiple mRNAs which arise by alternative processing of a common pre-mRNA precursor. It is not known how the pre-mRNA processing pathways are determined or controlled. We are investigating this problem by using the E3 complex transcription unit of adenovirus as a model. Our approach is to construct virus mutants with lesions in E3 and then determine how the mutation affects the accumulation of E3 mRNAs in vivo. We report results which indicate that competition between splicing reactions and polyadenylation reactions occurs in vivo and that this plays an important role in alternative pre-mRNA processing.
Brady
H A
HA
Institute for Molecular Virology, St. Louis University School of Medicine, Missouri 63110.
Wold
W S
WS
D017456:Adenovirus Early Proteins; D000260:Adenoviruses, Human / Q000235:genetics*; D006801:Humans; D007624:KB Cells; D009154:Mutation; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D010957:Plasmids; D011061:Poly A / Q000235:genetics*; D012313:RNA / Q000235:genetics*; D012322:RNA Precursors / Q000235:genetics; D012323:RNA Processing, Post-Transcriptional; D012326:RNA Splicing; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*
D017456:Adenovirus Early Proteins; D009856:Oncogene Proteins, Viral; D012322:RNA Precursors; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA