Size distribution of rhodamine-labelled microspheres retrogradely transported in cultured neurons.
Rhodamine-labelled latex microspheres (RLM), 10-110 nm in diameter, were used as a retrograde tracer in cultured sympathetic neurons. Retrogradely transported RLM were isolated from the cells and measured. These were all smaller than 50 nm in diameter and represented not more than 2% of the original RLM population. The permeability of RLM as a function of size was also estimated and showed a steep decrease between 20 and 30 nm from 100% to less than 20%. Therefore, RLM with a mean diameter of 20 nm would be optimal for retrograde tracing.
Holländer
H
H
Max-Planck-Institut für Psychiatrie, Planegg-Martinsried, F.R.G.
Egensperger
R
R
Dirlich
G
G
D000818:Animals; D001370:Axonal Transport; D002478:Cells, Cultured; D005456:Fluorescent Dyes; D005728:Ganglia, Sympathetic / Q000166:cytology / Q000378:metabolism*; D008863:Microspheres; D051381:Rats; D012235:Rhodamines; D014966:Xanthenes
D005456:Fluorescent Dyes; D012235:Rhodamines; D014966:Xanthenes