Antigenic reactivity of matrix protein and nucleoprotein of influenza virus as detected by EIA after dissociation with different detergents.
Solid phase enzyme-immunoassay (EIA) was employed to assess the antigenic reactivity of matrix protein (M) and nucleoprotein (NP) of influenza A virus adsorbed to polystyrene in the presence of different detergents such as beta-octaglucoside (OG), Triton X-100, Tween-20, sodium dodecylsulphate (SDS), sodium deoxycholate (Doch-Na), Nonidet P-40 (NP-40), and sarcosyl at concentrations ranging from 0 to 2%. The antigenic reactivity of NP was the highest in the absence of detergents. For M protein, Doch-Na, SDS, NP-40 and sarcosyl of 0.05-0.1% enhanced the chromatophoric response in EIA 1.5-2 times. In contrast, the antigenic reactivity of M protein remained unchanged after OG or Triton X-100 treatments, and it decreased in the presence of Tween-20.
Khristova
M L
ML
Ivanovsky Institute of Virology, Academy of Medical Science U.S.S.R., Moscow.
Busse
T L
TL
Egorenkova
E M
EM
Leonov
S V
SV
Sokolova
M V
MV
Gitelman
A K
AK
Herrmann
J
J
Döhner
L
L
Kharitonenkov
I G
IG
D000327:Adsorption; D000956:Antigens, Viral / Q000276:immunology*; D003902:Detergents; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D007124:Immunoenzyme Techniques; D009980:Influenza A virus / Q000187:drug effects / Q000276:immunology*; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000032:analysis* / Q000276:immunology* / Q000302:isolation & purification; D016601:RNA-Binding Proteins; D013501:Surface-Active Agents; D014758:Viral Core Proteins; D014763:Viral Matrix Proteins / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*
D000956:Antigens, Viral; D003902:Detergents; D000939:Epitopes; C031540:M-protein, influenza virus; C048842:NP protein, Influenza A virus; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D016601:RNA-Binding Proteins; D013501:Surface-Active Agents; D014758:Viral Core Proteins; D014763:Viral Matrix Proteins; D014764:Viral Proteins