The isolation of recombinant RNA species responsive to oestrogen and tamoxifen in rat uterus and MCF-7 cells.
We have previously shown that oestrogen elicits profound effects on the mRNA population of the immature rat uterus. In order to follow these responses in a more precise manner, we have prepared a cDNA library to uterine mRNA and screened this for sequences most responsive to the hormone. The stimulatory effect of oestrogen and tamoxifen on the levels of selected uterine mRNA species has been compared with the effect of the hormone and its agonist on total uterine poly(A)+ RNA levels. Two of the selected recombinant species were also expressed in the MCF-7 breast carcinoma cell line where their relative levels were again stimulated by oestrogen. Tamoxifen greatly reduced the poly(A)+ RNA levels of MCF-7 cells.
Travers
M T
MT
Department of Biochemistry, University of Glasgow, Scotland, U.K.
Wakeling
A E
AE
Knowler
J T
JT
D000818:Animals; D002460:Cell Line; D004965:Estrogen Antagonists / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008325:Mammary Neoplasms, Experimental / Q000188:drug therapy / Q000473:pathology; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D051381:Rats; D013629:Tamoxifen / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects*; D014599:Uterus / Q000187:drug effects*
D004965:Estrogen Antagonists; D004967:Estrogens; C039919:RNA, recombinant; D013629:Tamoxifen; D012313:RNA