[Expression of ribosomal genes under the action of 5-azacytidine in the African green monkey RAMT cell line].
The use of cytosine analogue--5-Azacytidine(5AzaC), derepression of ribosomal genes has been studied in one of organising chromosomes in the African green monkey RAMT cell line in which the nucleolar organizer region (NOR) in parental cells was active. The effect of 5AzaC on the functional state of NOR was assessed by the length of the secondary constriction in this chromosome and by the intensity of Ag-staining of NOR. 5AzaC was added to the cell culture at concentrations 2-16 M, either immediately after the cell passage or at the 24th h from the beginning of cell cultivation for the following 17-34 hours. As a control the cells cultivated in the absence of 5AzaC were used. Comparison of control cells with those treated with 5AzaC showed: 1) increase of the length of the second constriction in the chromosome with the initial inactive NOR in the 5AzaC--treated cells; 2) a marked increase of the intensity of NOR's Ag-staining in the same chromosome after incorporation of 5-AzaC into DNA. The conclusion about the methylation of cytosine bases in the DNA of ribosomal genes in one NOR organising chromosomes in RAMT cell line was made.
Baranovskaia
L I
LI
Liapunova
N A
NA
D000818:Animals; D001374:Azacitidine / Q000494:pharmacology*; D002460:Cell Line; D002522:Chlorocebus aethiops; D002875:Chromosomes / Q000187:drug effects; D004275:DNA, Ribosomal / Q000187:drug effects / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects*; D009697:Nucleolus Organizer Region / Q000187:drug effects; D013194:Staining and Labeling; D013997:Time Factors
D004275:DNA, Ribosomal; D001374:Azacitidine
Izuchenie ékspressii ribosomnykh genov pod vliianiem vozdeĭstviia 5-azatsitidina v linii kletok RAMT afrikanskoĭ zelenoĭ martyshki.