Abnormal distribution of CD8 subpopulation in B-chronic lymphocytic leukemia identified by flow cytometry.
We studied the occurrence of T-cell subpopulations for patients with B-cell chronic lymphocytic leukemia. The CD8+ population was divided into CD8+ suppressor (CD8a+) and CD8+ cytotoxic (CD8b+) lymphocytes using difference in orthogonal light scattering. Average CD4+/CD8+ ratios determined for all patients were decreased. For individual patients this sometimes was not true. In contrast CD4+/CD8a+ ratios were markedly increased in all individual patients. The CD8+ lymphocytes appeared to consist mainly of CD8b+ lymphocytes. Moreover the CD8b+/CD8+ ratio correlated with clinical stage: untreated patients (stage 0 of Rai) have smaller CD8b+/CD8+ ratios than patients with advanced stages of Rai.
Terstappen
L W
LW
Twente University of Technology, Department of Applied Physics, Enschede, The Netherlands.
de Grooth
B G
BG
van Berkel
W
W
ten Napel
C H
CH
Greve
J
J
D000328:Adult; D000368:Aged; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D001402:B-Lymphocytes / Q000032:analysis / Q000145:classification*; D002469:Cell Separation / Q000379:methods; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology* / Q000473:pathology; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D013194:Staining and Labeling; D013601:T-Lymphocytes / Q000145:classification
D000944:Antigens, Differentiation, B-Lymphocyte