Human beta 2-microglobulin specifically enhances cell-surface expression of HLA class I molecules in transfected murine cells.
Sequential transfections of P815 murine mastocytoma cells with class I gene encoding either HLA-Cw3, HLA-A3, or HLA-B7 H chain and subsequently with a human beta 2-microglobulin gene were performed to evaluate the relative efficiency of human and murine beta 2-microglobulins in promoting the cell-surface expression of HLA-class I molecules. A 6-, 11-, and 40-fold specific enhancement of the cell-surface expression of HLA-Cw3, HLA-A3, and HLA-B7 molecules, respectively, was observed in cells co-transfected with human beta 2-microglobulin gene. This effect was attributed to a more efficient association of HLA H chains with human than with murine beta 2-microglobulin, which apparently allowed a more rapid transport of the HLA molecules from the endoplasmic reticulum to the Golgi apparatus.
Perarnau
B M
BM
Centre d'Immunologie Institut National de la Santé et de la Recherche Médicale, Marseille, France.
Gillet
A C
AC
Hakem
R
R
Barad
M
M
Lemonnier
F A
FA
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D006680:HLA Antigens / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015793:HLA-B7 Antigen; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000302:isolation & purification; D051379:Mice; D011233:Precipitin Tests; D011499:Protein Processing, Post-Translational; D012313:RNA / Q000302:isolation & purification; D014162:Transfection; D001613:beta 2-Microglobulin / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*
D000954:Antigens, Surface; D000939:Epitopes; D006680:HLA Antigens; D015793:HLA-B7 Antigen; D007143:Immunoglobulin Heavy Chains; D001613:beta 2-Microglobulin; D012313:RNA