Permanent metachromatic effect of 1,9-dimethyl-methylene blue on acidic mucopolysaccharides of mastocytes.
The 1,9-dimethyl-methylene blue (SERVA) has a very strong metachromatic reaction with sulphated mucopolysaccharides of mastocytes at pH = 1 (Tris-HCl buffer). This metachromasia is retained also at increasing pH (2.5-5), and is not affected either by the quality or by the concentration of the ions present (1/240 M Tris-HCl, and 0.2 M phosphate buffer). The smears do not need any special mounting medium, only Canada balsam. The metachromasia is preserved for a considerable length of time.
Pokorny
E
E
National Institute of Oncology, Budapest, Hungary.
Kremmer
T
T
Holczinger
L
L
D000818:Animals; D006025:Glycosaminoglycans / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008407:Mast Cells / Q000032:analysis*; D008751:Methylene Blue / Q000031:analogs & derivatives*; D051379:Mice; D013194:Staining and Labeling
D006025:Glycosaminoglycans; D008751:Methylene Blue; C016401:1,9-dimethylmethylene blue