Decreased cyclic AMP accumulation in lymphocytes in response to adrenaline and prostacyclin after n-3 polyunsaturated fatty acid supplementation in man.
The effect of supplementation with n-3 polyunsaturated fatty acids on beta-adrenoceptor function in lymphocytes has been studied in ten healthy male volunteers. Ten Max-Epa capsules containing 320 mg n-3 polyunsaturated fatty acids per capsule were given for 3 weeks, and the cyclic AMP accumulation response in lymphocytes to adrenaline and the prostacyclin analogue iloprost (ZK 36374) were assessed before and after supplementation. After supplementation about 30% less cAMP was accumulation by the lymphocytes in response to either adrenaline or iloprost. Propranolol inhibited the adrenaline-induced increase in cAMP both before and after supplementation, but the difference in the basal cAMP concentration between the groups still persisted. Adrenaline stimulation after pre-incubation of the lymphocytes with the alpha-adrenoceptor antagonist phentolamine resulted in an even more pronounced difference between pre- and post-supplementation cAMP concentrations. The results suggest that fish oil supplementation may lead to decreased responsiveness of adenylate cyclase to catecholamine and prostaglandin stimulation.
Laustiola
K E
KE
Wihuri Research Institute, Helsinki, Finland.
D000328:Adult; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D000242:Cyclic AMP / Q000378:metabolism*; D004837:Epinephrine / Q000494:pharmacology*; D011464:Epoprostenol / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005395:Fish Oils / Q000494:pharmacology*; D006801:Humans; D016285:Iloprost; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010646:Phentolamine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D014280:Triglycerides / Q000097:blood
D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D005231:Fatty Acids, Unsaturated; D005395:Fish Oils; D007457:Iodine Radioisotopes; D014280:Triglycerides; D002784:Cholesterol; D011433:Propranolol; D011464:Epoprostenol; D000242:Cyclic AMP; D016285:Iloprost; D004837:Epinephrine; D010646:Phentolamine