title: Interaction of neuropeptides with human mast cells.
abstract: Mast cells of human skin, but not lung, adenoids, tonsils, or intestine, release histamine in response to substance P, vasoactive intestinal polypeptide, and somatostatin. The substance P receptor of skin mast cells is not of the NK-1, NK-2 or NK-3 subtypes of smooth muscle. Time course and calcium dependency of release by peptides differed from anti-IgE. With anti-IgE, the molar ratios of histamine:PGD2:LTC4 generated by skin mast cells was 1,000:25:2, whereas with substance P these ratios were 1,000:1:0.1. Similar results were obtained with the other neuropeptides. The ability of peptides to stimulate skin mast cell histamine release suggests a mechanism whereby their release from dermal nerve endings is coupled to changes in microvasculature.
authors: Clinical Pharmacology and Medicine I, Southampton General Hospital, UK.
authors: M K
authors: MK
authors: Church
authors: M A
authors: MA
authors: Lowman
authors: C
authors: C
authors: Robinson
authors: S T
authors: ST
authors: Holgate
authors: R C
authors: RC
authors: Benyon
mesh_terms: D006636:Histamine Release; D006801:Humans; D007073:Immunoglobulin E / Q000502:physiology; D066298:In Vitro Techniques; D007249:Inflammation / Q000503:physiopathology*; D008407:Mast Cells / Q000502:physiology*; D009479:Neuropeptides / Q000502:physiology*; D015230:Prostaglandin D2 / Q000096:biosynthesis; D013189:SRS-A / Q000096:biosynthesis; D012867:Skin / Q000166:cytology; D013373:Substance P / Q000502:physiology
chemical_list: D009479:Neuropeptides; D013189:SRS-A; D013373:Substance P; D007073:Immunoglobulin E; D015230:Prostaglandin D2