Mouse hair keratin cDNA: implications and sequences.
An efficient method is described for the isolation of mouse hair follicle mRNAs. The RNA contains two peaks of mRNA activity, 11S and 18S. The larger-sized group of mRNAs encodes keratins of 46K, 47K, 58K, 59K, and 63K Mr. cDNAs were prepared using the follicular mRNAs as templates and cloned to produce a library. Initial screening of this library has identified five acidic and six basic mouse hair keratin clones.
Bertolino
A P
AP
Department of Dermatology, New York University Medical Center, New York.
D000818:Animals; D003001:Cloning, Molecular; D015342:DNA Probes; D006197:Hair / Q000032:analysis*; D007633:Keratins / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D012313:RNA / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000032:analysis; D012867:Skin / Q000032:analysis
D015342:DNA Probes; D012333:RNA, Messenger; D012313:RNA; D007633:Keratins