Demonstration and elimination of artifacts associated with gold staining of proteins on nitrocellulose membranes after separation by sodium dodecyl sulphate-polyacrylamide gel electrophoresis.
Artifacts associated with gold staining of polypeptides separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and transferred to nitrocellulose are eliminated by alkylation of reduced samples or by deletion of the reducing agent.
Bath
M L
ML
Department of Immunology, John Curtin School of Medical Research, Australian National University, Canberra.
D000478:Alkylation; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D006046:Gold; D008567:Membranes, Artificial; D011506:Proteins / Q000032:analysis*; D015203:Reproducibility of Results; D013194:Staining and Labeling
D008567:Membranes, Artificial; D011506:Proteins; D006046:Gold; D003101:Collodion