A vector that expresses secreted proteins on the cell surface.
A new vector, CDM8PI, has been constructed. It was derived from the plasmid expression vector CDM8, which has been used in the epitope-loss mutant isolation technique to map the epitopes on cell-surface proteins. The new vector allows the production of fusion proteins between normally secreted proteins and the membrane anchor moiety from a cell-surface protein, LFA-3, thereby expressing the fusion proteins on the cell surface. The vector extends the application of the epitope-loss mutant isolation technique to secreted proteins. The vector also allows the easy recovery of mutated proteins in unfused forms after the immunoselection and characterization.
Wang
X B
XB
Laboratory of Lipoprotein Metabolism, Institut de Recherches Cliniques de Montreal, Quebec, Canada.
Milne
R
R
Marcel
Y
Y
Rassart
E
E
D000595:Amino Acid Sequence; D000954:Antigens, Surface / Q000235:genetics*; D001483:Base Sequence; D018968:CD58 Antigens; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D005822:Genetic Vectors; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis; D013728:Terminator Regions, Genetic; D014162:Transfection
D000954:Antigens, Surface; D018968:CD58 Antigens; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins