Low molecular weight RNA of Drosophila cells which is induced by heat shock--II. Structural properties.
Heat shock-induced small cytoplasmic RNA (HSI scRNA) from Drosophila culture cell was larger in size after dimethylchloroacetal treatment than in 7 M urea. Judging from its thermal denaturation profile and the nuclease S1 digestion experiment it was supposed that HSI scRNA is double-stranded. Nucleotide sequence analysis of the scRNA also suggested that it is double-stranded RNA with the blunt-ends.
Kawata
Y
Y
Department of Biology, College of Arts and Sciences, University of Tokyo, Japan.
Fujiwara
H
H
Shiba
T
T
Miyake
T
T
Ishikawa
H
H
D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D002478:Cells, Cultured; D004331:Drosophila melanogaster / Q000378:metabolism*; D006358:Hot Temperature / Q000009:adverse effects; D015394:Molecular Structure; D008970:Molecular Weight; D012313:RNA / Q000096:biosynthesis*; D012330:RNA, Double-Stranded / Q000096:biosynthesis; D020733:RNA, Small Cytoplasmic
D012330:RNA, Double-Stranded; D020733:RNA, Small Cytoplasmic; D012313:RNA