Interferon beta 2/B-cell stimulating factor 2/interleukin 6 affects glycosylation of acute phase proteins in human hepatoma cell lines.
Undefined monocyte-derived cytokines have previously been shown to affect glycan processing in glycoproteins secreted by human hepatoma cell lines. Hep 3B cells, when incubated with the cytokine interferon beta 2/B-cell stimulating factor 2/interleukin 6, secreted forms of alpha 1-protease inhibitor, ceruloplasmin, and alpha-fetoprotein with increased reactivity with concanavalin A (Con A) while incubation of Hep G2 cells with this cytokine led to secretion of forms of these proteins with decreased reactivity with Con A, reflecting changes in their oligosaccharide chains. The difference in response of these two transformed cell lines to this cytokine undoubtedly reflects differences in their intracellular glycan processing mechanisms. Changes in glycosylation patterns were dissociated from changes in rate of synthesis: this cytokine caused increased synthesis of alpha 1-protease inhibitor and ceruloplasmin, and decreased synthesis of alpha-fetoprotein in both cell lines.
Mackiewicz
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A
Department of Immunology and Rheumatology, K. Marcinkowski Academy of Medicine, Poznan, Poland.
Kushner
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I
D000209:Acute-Phase Proteins / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002460:Cell Line; D002570:Ceruloplasmin / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D006031:Glycosylation; D006801:Humans; D015850:Interleukin-6; D007378:Interleukins / Q000494:pharmacology*; D008113:Liver Neoplasms / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism; D000515:alpha 1-Antitrypsin; D000509:alpha-Fetoproteins / Q000378:metabolism
D000209:Acute-Phase Proteins; D001798:Blood Proteins; D015850:Interleukin-6; D007378:Interleukins; D000515:alpha 1-Antitrypsin; D000509:alpha-Fetoproteins; D002570:Ceruloplasmin