[Experimental studies on the pathogenesis and development of interstitial pneumonia and pulmonary fibrosis].
In order to elucidate the mechanism of pulmonary fibrosis development, analysis of bronchoalveolar lavage fluid (BALF), alveolar macrophage function and histological examination were performed in bleomycin (BLM) treated mice and MRL/lpr mice. In the BLM-treated group, swelling of type II alveolar cells, increase of phospholipids and total cell counts in BALF were observed at the early stage following BLM administration. Lysosomal enzyme activities, total protein volume, superoxide production and IL-1 production of alveolar macrophages peaked at the second week after completing BLM administration. In MRL/lpr mice, lymphocytes infiltration in the interstitium, perivascular and peribronchial granuloma formation were observed at 24 weeks old. Analysis of BALF revealed the increase of total cell counts, lymphocyte counts, and fibronectin volume. No significant difference was observed in total protein and phospholipid volume between MRL/lpr mice and MRL/n mice (control mice).
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D000818:Animals; D001761:Bleomycin; D001992:Bronchoalveolar Lavage Fluid / Q000032:analysis / Q000166:cytology*; D005353:Fibronectins / Q000032:analysis; D007958:Leukocyte Count; D008264:Macrophages / Q000502:physiology; D051379:Mice; D011650:Pulmonary Alveoli / Q000166:cytology; D011658:Pulmonary Fibrosis / Q000139:chemically induced / Q000209:etiology* / Q000473:pathology
D005353:Fibronectins; D001761:Bleomycin