title: The muscarinic receptor of rat pituitary GH3 cells is coupled with adenylate cyclase inhibition, but not with phosphoinositide turnover.
abstract: The effects of muscarinic stimulation on cyclic AMP accumulation and on basal and thyrotropin-releasing hormone (TRH)-induced phosphoinositide turnover have been studied in rat pituitary GH3 tumour cells. Carbachol produced a dose-dependent atropine-sensitive inhibition of basal cyclic AMP accumulation. It had no effect on basal or TRH-stimulated production of inositol phosphates. The majority of the muscarinic receptors had a low affinity (Ki 0.22 microM) for pirenzepine and can be classified as M2 type, inhibiting adenylate cyclase but having no links with phosphoinositide metabolism.
authors: Department of Biochemistry, University of Nottingham Medical School, Queen's Medical Centre, U.K.
authors: H
authors: H
authors: Yagisawa
authors: S H
authors: SH
authors: Simmonds
authors: J N
authors: JN
authors: Hawthorne
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D007295:Inositol Phosphates / Q000378:metabolism; D019832:N-Methylscopolamine; D010716:Phosphatidylinositols / Q000378:metabolism*; D010890:Pirenzepine / Q000494:pharmacology; D010902:Pituitary Gland / Q000502:physiology*; D051381:Rats; D011976:Receptors, Muscarinic / Q000502:physiology*; D012602:Scopolamine Derivatives / Q000378:metabolism; D013973:Thyrotropin-Releasing Hormone / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D007295:Inositol Phosphates; D010716:Phosphatidylinositols; D011976:Receptors, Muscarinic; D012602:Scopolamine Derivatives; D010890:Pirenzepine; D013973:Thyrotropin-Releasing Hormone; D002217:Carbachol; D000262:Adenylyl Cyclases; D015056:1-Methyl-3-isobutylxanthine; D019832:N-Methylscopolamine