title: O-antigenic chains of lipopolysaccharide prevent binding of antibody molecules to an outer membrane pore protein in Enterobacteriaceae.
abstract: The accessibility of outer membrane pore protein PhoE to antibody molecules at the cell surface of intact cells of various Enterobacteriaceae was investigated. Significant antibody binding was observed for only two of the nine strains tested. Analysis of the lipopolysaccharide by sodium dodecyl sulphate gel electrophoresis revealed a clear correlation between the presence of an O-antigenic side chain and the inability to bind PhoE protein-specific antibodies. As mutants that lack the O-antigen chain appeared to have acquired the ability to bind antibody, it must be concluded that the presence of O-antigenic chains of lipopolysaccharide prevents binding of antibodies to PhoE protein at the surface of intact cells. The relevance of this conclusion for the potential use of enterobacterial outer membrane pore proteins as vaccine components is discussed.
authors: Department of Molecular Cell Biology, State University, Utrecht, The Netherlands.
authors: P
authors: P
authors: van der Ley
authors: O
authors: O
authors: Kuipers
authors: J
authors: J
authors: Tommassen
authors: B
authors: B
authors: Lugtenberg
mesh_terms: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001665:Binding Sites; D003165:Complement System Proteins; D004755:Enterobacteriaceae / Q000235:genetics / Q000276:immunology; D008070:Lipopolysaccharides / Q000235:genetics / Q000276:immunology*; D009154:Mutation; D019081:O Antigens; D018272:Porins
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D008070:Lipopolysaccharides; D019081:O Antigens; D018272:Porins; D003165:Complement System Proteins