Cellular immunity to bovine myelin basic protein in rats bearing malignant fibrous histiocytoma.
T lymphocytes from rats with malignant fibrous histiocytoma at different stages of tumor growth were investigated for sensitization to bovine myelin basic protein (MBP). The immunological reactivity was assessed by a leukocyte adherence inhibition assay as an in vitro correlate of cellular immunity. We found that only T lymphocytes obtained 4 and 6 days after tumor transplantation are reactive to MBP (1-2 micrograms/ml). Since the sequence of the antigenic determinants from MBP is known, our findings may make it possible to select synthetic epitopes for generating cytotoxic T lymphocytes to these tumor cells.
Ribeiro
R A
RA
Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceara, Fortaleza, CE, Brasil.
Lopes-Alberto
F
F
De-Lucca
F L
FL
D000818:Animals; D018219:Histiocytoma, Benign Fibrous / Q000276:immunology*; D007111:Immunity, Cellular; D007957:Leukocyte Adherence Inhibition Test; D004676:Myelin Basic Protein / Q000276:immunology*; D009368:Neoplasm Transplantation; D051381:Rats; D011919:Rats, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology*
D004676:Myelin Basic Protein