title: Evidence for the expression of a primitive intestinal-like alkaline phosphatase in the intestinal 407 cell line.
abstract: Intestinal-like alkaline phosphatase was found to be expressed in the intestinal 407 cell line. This enzyme was identified by use of monoclonal antibodies specific for human placental (H7 and HPMS-1) and intestinal alkaline phosphatase (2HIMS-1 and 2HIMS-3) separately. Purification of this isozyme by use of two different monoclonal antibody immunoaffinity chromatographies demonstrates a single protein band on SDS-polyacrylamide gel electrophoresis indicating that this enzyme is not formed as a heterodimer. The apparent monomer subunit molecular weight and the dimer molecular weight of this isozyme were determined to 70000 and 160000, respectively. The enzyme is a homodimer according to molecular weight determinations. Furthermore, this isozyme is neuraminidase sensitive and comparatively heat stable, properties also characteristic for the placental enzyme. Our data suggest that the intestinal-like alkaline phosphatase in the intestinal 407 cell line displays properties intermediate of the intestinal and placental isozymes which may reflect the existence and reexpression of a new primitive isozyme.
authors: Gifu Pharmaceutical University, Japan.
authors: K
authors: K
authors: Hirano
authors: T
authors: T
authors: Kusano
authors: K
authors: K
authors: Asai
authors: K
authors: K
authors: Hayashi
authors: T
authors: T
authors: Stigbrand
authors: S
authors: S
authors: Iino
authors: J L
authors: JL
authors: Millan
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex / Q000032:analysis; D002460:Cell Line; D000939:Epitopes / Q000032:analysis; D005260:Female; D006801:Humans; D007422:Intestines / Q000201:enzymology*; D007527:Isoenzymes / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D008099:Liver / Q000201:enzymology; D008970:Molecular Weight; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy
chemical_list: D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000939:Epitopes; D007527:Isoenzymes; D000469:Alkaline Phosphatase