Mapping of cat albumin using monoclonal antibodies: identification of determinants common to cat and dog.
Cat and dog albumins from commercial extracts were used to produce monoclonal antibodies (MoAb). Anti-cat albumin MoAb recognized both cat and dog albumin equally, as did anti-dog albumin MoAb; this confirms cross-reactivity between cat and dog. The MoAb were separated into two groups according to their epitopic specificity; they recognized two overlapping epitopes of cat albumin. Furthermore, by competitive inhibition of radio-allergosorbent test (RAST), it was shown that one MoAb group inhibited significantly the binding of human IgE antibodies (from a pool of 13 patients allergic to both cats and dogs) to insolubilized cat or dog extracts. These observations suggest that murine anti-cat or anti-dog MoAb and human IgE antibodies recognize identical or closely related determinants on cat and dog albumin.
Boutin
Y
Y
Unité de recherche Inflammation et Immunologie-Rhumatologie, Centre Hospitalier de l'Université Laval, Ste-Foy, Québec, Canada.
Hébert
J
J
Vrancken
E R
ER
Mourad
W
W
D000418:Albumins / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002415:Cats / Q000276:immunology*; D003429:Cross Reactions; D004285:Dogs; D000939:Epitopes / Q000032:analysis* / Q000276:immunology; D011852:Radioallergosorbent Test; D013045:Species Specificity
D000418:Albumins; D000911:Antibodies, Monoclonal; D000939:Epitopes