A redetermination of the concentration of alpha 2-macroglobulin in human plasma.
The concentration of alpha 2-macroglobulin in human plasma has been remeasured utilizing a carefully isolated and characterized sample of alpha 2-macroglobulin as a standard. A highly purified sample of alpha 2-macroglobulin with a total trypsin binding capacity of 1.7 mol trypsin/mol alpha 2-macroglobulin was used as a standard for both a radial immunodiffusion and a rocket immunoelectrophoresis technique. With this preparation as a standard, the concentration of alpha 2-macroglobulin in a normal plasma pool over 10,000 donors was found to be about 1.2 mg/ml. A similar concentration (1.3 mg/ml) was found when using a functional trypsin binding assay. This concentration is considerably less than the usually accepted mean of the normal range for alpha 2-macroglobulin.
Coan
M H
MH
Cutter Laboratories Inc., Berkeley, California.
Roberts
R C
RC
D000293:Adolescent; D000328:Adult; D000375:Aging / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D005779:Immunodiffusion; D007123:Immunoelectrophoresis, Two-Dimensional; D008875:Middle Aged; D014361:Trypsin Inhibitors; D000511:alpha-Macroglobulins / Q000032:analysis*
D014361:Trypsin Inhibitors; D000511:alpha-Macroglobulins