Human alpha 2-macroglobulin as an inhibitor of insoluble trypsin.
alpha 2-Macroglobulin binds to insoluble trypsin bound on agarose beads inducing a reduction of proteolytic activity of the enzyme towards large substrates such as azocasein. When trypsin was bound on other matrices like sheep red blood cells or latex beads, the inhibition of proteolytic activity by alpha 2-macroglobulin was complete. These results show that alpha 2-macroglobulin inhibits similarly both soluble and insoluble proteinases.
Heumann
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D
Division of Rheumatology, Hôpital Cantonal Universitaire, Geneve, Switzerland.
Burger
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D
Vischer
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TL
D004800:Enzymes, Immobilized / Q000037:antagonists & inhibitors*; D006801:Humans; D007700:Kinetics; D011485:Protein Binding; D014357:Trypsin / Q000378:metabolism*; D014361:Trypsin Inhibitors; D000511:alpha-Macroglobulins / Q000302:isolation & purification / Q000494:pharmacology*
D004800:Enzymes, Immobilized; D014361:Trypsin Inhibitors; D000511:alpha-Macroglobulins; D014357:Trypsin