Immunoelectron microscopic studies of GH and alpha subunit in GH secreting pituitary adenomas.
9 of 15 cases of GH secreting adenomas showed the localization of GH and a subunit in the adenoma cells. GH and a subunit were frequently colocalized in the same adenomas. Immunoelectron microscopically, GH and a subunit were localized in secretory granules which were packed in the cytoplasm. Immunoelectron microscopic double staining (preembedding method and post-embedding method) showed colocalization of GH and a subunit in the same secretory granules. These data suggested corelease of GH and a subunit by the same secretory granules.
Osamura
R Y
RY
Department of Pathology, Tokai University School of Medicine, Japan.
D000236:Adenoma / Q000378:metabolism / Q000648:ultrastructure*; D015292:Glycoprotein Hormones, alpha Subunit / Q000032:analysis*; D013006:Growth Hormone / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D010911:Pituitary Neoplasms / Q000378:metabolism / Q000648:ultrastructure*
D015292:Glycoprotein Hormones, alpha Subunit; D013006:Growth Hormone