Identification of a highly abundant cDNA isolated from senescent WI-38 cells.
A cDNA library was constructed from poly(A)+ RNA derived from late passage WI-38 cells and differentially screened with cDNA probes from early and late passage cells. From a number of clones which exhibited differences in intensity of hybridization to the early or late passage probes, one was chosen for further analysis because of its large increase in hybridization to the late passage probe. This clone accounted for approximately 1% of the recombinants in the library. The partial cDNA clone shows complete sequence homology to elongation factor I alpha (EF-I alpha). Northern analysis of poly(A)+ RNA from cells at various population doublings suggested that a 2.2-kb transcript, homologous to EF-I alpha accumulates as cells near the end of their replicative life span (phase III). When early and late passage cells were reexposed to serum after serum starvation, this transcript decreased in abundance. Additionally, a lower molecular weight transcript (1.6 kb) was detected 18 h following serum stimulation in early passage cells and 9 h after stimulation in late passage cells.
Giordano
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Laboratory of Molecular Biology, National Institutes of Health, Bethesda, Maryland 20892.
Foster
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D001483:Base Sequence; D002453:Cell Cycle; D002460:Cell Line; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D005333:Fetus; D006801:Humans; D008168:Lung; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000235:genetics*; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger; D012261:Ribonucleoproteins / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D011061:Poly A; D012313:RNA; D004247:DNA