Effects of nitrogen mustard-N-oxide on ionic activities of inner ear fluid and ionic permeabilities of the cochlear partition in the guinea pig.
The effect of nitrogen mustard-N-oxide (NMO) on the endocochlear potential (EP) was investigated from the aspect of the ion concentrations and permeabilities in the cochlea. Compared with the untreated animals, in NMO-treated animals 20 to 30 hours after administration, the EP was decreased (30.8 +/- 3.5 mV in NMO versus 82.4 +/- 1.6 mV in control), the K+ concentration in perilymph of the scala tympani was increased (8.2 +/- 1.0 mM versus 5.3 +/- 0.7 mM), the K+ concentration in endolymph was decreased (128.5 +/- 10.6 mM versus 157.9 +/- 7.9 mM), and the Na+ concentration in endolymph was increased (9.6 +/- 3.6 mM versus 2.5 +/- 0.4 mM). The permeability coefficient for Na+ of the cochlear partition in the NMO-treated animals significantly decreased, while that for Cl- significantly increased. The negative EP, which presumably exists in the normal state, diminished further (-2.7 mV versus -27.8 mV), and the calculated electrogenic potential of the EP was depressed remarkably (33.5 mV versus 110.2 mV). The results suggest that the effects of NMO involved changes in ion permeabilities of the partition and the inhibition of electrogenic transport processes in the cochlea.
Ikeda
K
K
Department of Otolaryngology, Tohoku University School of Medicine, Sendai, Japan.
Kusakari
J
J
Takasaka
T
T
Saito
Y
Y
D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D003051:Cochlea / Q000187:drug effects*; D003055:Cochlear Microphonic Potentials / Q000187:drug effects*; D005072:Evoked Potentials, Auditory / Q000187:drug effects*; D006168:Guinea Pigs; D007473:Ion Channels / Q000187:drug effects*; D007761:Labyrinthine Fluids / Q000187:drug effects*; D008466:Mechlorethamine; D009588:Nitrogen Mustard Compounds / Q000633:toxicity*
D000970:Antineoplastic Agents; D007473:Ion Channels; D009588:Nitrogen Mustard Compounds; D008466:Mechlorethamine