Inhibition of adenovirus 5 replication in COS-1 cells by antisense RNAs against the viral E1a region.
To study the effect of antisense E1a RNA (asRNA) on adenovirus development, two types of adenovirus 5 E1a antisense constructs have been engineered. One was complementary to the viral DNA region [nucleotide (nt) positions 500-720] regulated by the metallothionein-I promoter, and the other was complementary to the DNA regions (nt positions 630-1570) under control of the long terminal repeat Moloney mouse leukosis virus promoter. Both asRNA constructs were cloned into a plasmid containing the simian virus 40 origin of replication, the gene controlling geneticin (G418) resistance (G418R), and other regulatory elements. The COS-1 cells, which contained up to 100 copies of the engineered plasmids, synthesized antiviral asRNAs, which provided 71 to over 95% inhibition of adenoviral replication, in comparison to the control cells not synthesizing asRNAs.
Miroshnichenko
O I
OI
Institute of Agricultural Biotechnology, USSR Academy of Agricultural Sciences, Moscow.
Ponomareva
T I
TI
Tikchonenko
T I
TI
D017456:Adenovirus Early Proteins; D000260:Adenoviruses, Human / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D000818:Animals; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics*; D015870:Gene Expression; D005814:Genes, Viral; D006801:Humans; D008668:Metallothionein / Q000235:genetics; D008979:Moloney murine leukemia virus / Q000235:genetics; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012313:RNA / Q000235:genetics / Q000494:pharmacology*; D016372:RNA, Antisense; D012333:RNA, Messenger / Q000037:antagonists & inhibitors*; D015183:Restriction Mapping; D014162:Transfection; D015678:Viral Structural Proteins / Q000235:genetics; D014779:Virus Replication / Q000187:drug effects*
D017456:Adenovirus Early Proteins; D004268:DNA-Binding Proteins; D009856:Oncogene Proteins, Viral; D016372:RNA, Antisense; D012333:RNA, Messenger; D015678:Viral Structural Proteins; D012313:RNA; D008668:Metallothionein