[Prostate-specific antigen (PA) and deoxyribonucleic acid (DNA) analysis in prostatic epithelial cells using flow cytometry].
We developed a new method to analyze simultaneously cellular prostate-specific antigen (PA) content and deoxyribonucleic acid (DNA) content of each prostatic epithelial cell by using flow cytometry (FCM). We report the possibilities of this method and we analyzed firstly 8 samples from 6 patients with benign prostatic hypertrophy (BPH). The content of cellular PA and DNA of prostatic epithelial cells can be analyzed simultaneously. The PA is synthesized only in the cells with a 2C DNA content (G0/1 cells) and not in the S- or G2M-phase cells. In the near future, by applying this analytical method to prostatic cancer samples we could obtain more interesting knowledge of the behavior of prostatic cancer.
Shimabukuro
T
T
Department of Urology, Yamaguchi University School of Medicine.
Yoshihiro
S
S
Matsuyama
H
H
Yamamoto
N
N
Sakatoku
J
J
D000368:Aged; D000951:Antigens, Neoplasm; D004247:DNA / Q000032:analysis*; D004847:Epithelial Cells; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D008297:Male; D008875:Middle Aged; D011467:Prostate / Q000166:cytology / Q000276:immunology*; D017430:Prostate-Specific Antigen; D011470:Prostatic Hyperplasia / Q000473:pathology
D000951:Antigens, Neoplasm; D004247:DNA; D017430:Prostate-Specific Antigen