Human normal CD5+ B lymphocytes can be induced to differentiate to CD5- B lymphocytes with germinal center cell features.
A significant proportion of cord blood CD5+ B cells express the activation molecules CD23, CD25, and transferrin receptor; react with the cell-cycle-associated monoclonal antibody (MoAb) Ki67; can be induced to enter the S phase of cell cycle by interleukin-2 (IL-2), IL-4, or low-molecular-weight B-cell growth factor (Imw-BCGF) and, exposed to IL-1 and IL-2, acquire the features (sIgD-, CD5-, CD10+, CD38+) of B blasts proliferating in the germinal centers of secondary follicles. These findings indicate that CD5+ B cells are preactivated and, in the proper microenvironment, may give rise to CD5- B cells.
Caligaris-Cappio
F
F
Dipartimento di Scienze Biomediche e Oncologia Umana, Università di Torino, Italy.
Riva
M
M
Tesio
L
L
Schena
M
M
Gaidano
G
G
Bergui
L
L
D000818:Animals; D000943:Antigens, Differentiation; D001402:B-Lymphocytes / Q000145:classification* / Q000187:drug effects / Q000502:physiology; D018956:CD5 Antigens; D002454:Cell Differentiation / Q000187:drug effects; D005312:Fetal Blood / Q000166:cytology; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D008221:Lymphoid Tissue / Q000166:cytology*; D010641:Phenotype; D011817:Rabbits; D013234:Stem Cells / Q000145:classification* / Q000187:drug effects / Q000502:physiology
D000943:Antigens, Differentiation; D018956:CD5 Antigens; D006133:Growth Substances