[Preparation of homologs of oligoribouridylic acid by selective partial hydrolysis of RNA].
The preparation of oligouridylic acids was achieved by the stepwise partial hydrolysis of RNA using enzymatical and chemical methods of degradation followed by chromatographic purification steps. After RNA is submitted first to RNase T1 and after that to RNase U2 it is cleaved into oligopyrimidine nucleotides carrying purine nucleotides only at their 3'-terminal. By chemical deamination the pyrimidine sequences are converted to oligouridine sequences. After enzymatical dephosphorylation of the oligouridylic acids their 3'-terminal purine nucleosides are eliminated by a periodate-lysine treatment. The phosphate groups remaining at the 3'-terminal of the resulting oligouridylic acids are enzymatically removed. As a result of the partial hydrolysis the mean recoveries of the particular homologues are U2-U7 93% and U8 86% adding up to about 0.5% of the amount of RNA used as starting material.
Schott
H
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Institut für Organische Chemie der Universität Tübingen, B.R.D.
D001483:Base Sequence; D002852:Chromatography, Ion Exchange / Q000379:methods; D004722:Endoribonucleases; D006868:Hydrolysis; D007202:Indicators and Reagents; D009843:Oligoribonucleotides / Q000302:isolation & purification*; D012313:RNA; D006163:Ribonuclease T1; D014500:Uracil Nucleotides / Q000302:isolation & purification*
D007202:Indicators and Reagents; D009843:Oligoribonucleotides; D014500:Uracil Nucleotides; C027916:oligo(U); D012313:RNA; D004722:Endoribonucleases; D006163:Ribonuclease T1; C022544:ribonuclease U2
Präparative Darstellung von Homologen der Oligoribouridylsäure durch selektive Partialhydrolyse von RNA.