In vitro studies of the interaction of heparin, low molecular weight heparin and heparinoids with platelets.
Heparin, low molecular weight heparins, and heparinoids were studied for their ability to inhibit the aggregation of platelets by various agonists and for their ability to adhere to collagen. Heparin was a very effective inhibitor of aggregation with collagen and with ristocetin as it was with adhesion to collagen. The heparinoids showed little effect on aggregation or adhesion. Heparan sulfate and pentosan polysulfate did show slight inhibitory activity against collagen aggregation and adhesion and both interacted with the antibody induced by heparin therapy. It is of interest that dermatan sulfate and the pentasaccharide were almost inert in these experiments, and are unlikely to induce bleeding by inhibition of platelet function. It is highly probable that interference with the interaction of von Willebrand factor with platelets and collagen is a major mechanism for bleeding in the heparinized patient.
Messmore
H L
HL
Department of Medicine, Hines VA Hospital, Illinois 60141.
Griffin
B
B
Fareed
J
J
Coyne
E
E
Seghatchian
J
J
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects* / Q000502:physiology; D002417:Cattle; D003094:Collagen / Q000378:metabolism / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology*; D006495:Heparin, Low-Molecular-Weight / Q000494:pharmacology*; D006496:Heparinoids / Q000494:pharmacology*; D006801:Humans; D010973:Platelet Adhesiveness / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D012310:Ristocetin / Q000494:pharmacology; D013917:Thrombin / Q000494:pharmacology
D006495:Heparin, Low-Molecular-Weight; D006496:Heparinoids; D010975:Platelet Aggregation Inhibitors; D012310:Ristocetin; D000244:Adenosine Diphosphate; D006493:Heparin; D003094:Collagen; D013917:Thrombin