title: A hybrid gene to express protein epitopes from both sporozoite and merozoite surface antigens of Plasmodium falciparum.
abstract: The DNA coding for parts of the repetitive amino acid sequence of Plasmodium falciparum circumsporozoite protein has been spliced to a sequence encoding part of the precursor to the major merozoite surface antigens, to produce a hybrid gene. Expression in Escherichia coli produces a protein with antigenic determinants from both malaria proteins. Antibodies raised against the expressed material react with both a peptide derived from the circumsporozoite repeat sequence, and the merozoite surface molecule. Hybrid molecules of this type may be the basis of a malaria vaccine.
authors: Department of Molecular Biology, Wellcome Research Laboratories, Beckenham, Kent.
authors: A A
authors: AA
authors: Holder
authors: M J
authors: MJ
authors: Lockyer
authors: G W
authors: GW
authors: Hardy
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000235:genetics; D005786:Gene Expression Regulation; D005813:Genes, Synthetic; D008969:Molecular Sequence Data; D010957:Plasmids; D010963:Plasmodium falciparum / Q000235:genetics / Q000276:immunology*; D011233:Precipitin Tests; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D000953:Antigens, Protozoan; D000954:Antigens, Surface; D000939:Epitopes; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D004247:DNA