Effect of anti-S-100 serum on 36Cl- ion permeability across the Deiters' neuron plasma membrane.
1. A microtechnique allowing the study of single plasma membranes from the gamma-aminobutyric acid (GABA)-acceptive Deiters' neuron has been utilized in order to assess the effect of both S-100 protein and its antiserum on 36Cl- permeability through such membranes. 2. The results show that both S-100 (in the Ca2+ form) incorporation onto the external side of the membranes and their preincubation with anti-S-100 serum stimulate 36Cl- permeability. 3. These effects are not additive with that of GABA, indicating that both S-100 and anti-S-100 act via the GABAA receptor complexes on Deiters' membranes. 4. When the membranes were incubated first with S-100/Ca2+ and then with anti-S-100, the second treatment resulted in the disappearance of the S-100 effect. However, the anti-S-100 effect was fully displayed.
Hydén
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H
Institute of Neurobiology, University of Göteborg, Sweden.
Cupello
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Palm
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D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects*; D002712:Chlorides / Q000378:metabolism*; D007106:Immune Sera / Q000494:pharmacology*; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism; D011817:Rabbits; D011963:Receptors, GABA-A / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D009418:S100 Proteins / Q000276:immunology* / Q000494:pharmacology / Q000502:physiology; D014726:Vestibular Nuclei / Q000378:metabolism*; D003689:Vestibular Nucleus, Lateral / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D002712:Chlorides; D007106:Immune Sera; D007473:Ion Channels; D011963:Receptors, GABA-A; D009418:S100 Proteins; D005680:gamma-Aminobutyric Acid