Expression of the DBA/2J Ren-2 gene in the adrenal gland of transgenic mice.
To characterize further the tissue-specific control of the mouse Ren-2 gene, and in particular its expression in the adrenal gland, we have introduced the DBA/2 Ren-2 gene into the genome of Ren-1c/Ren-1c mice. Here we report our observations on Ren-2 transgenic mice. Expression was found in the correct spectrum of tissues and included appropriate hormonal control in the submandibular gland. Quantitatively transcript levels varied both positively (adrenal gland and sex-accessory tissue) and negatively (submandibular gland and kidney) with respect to normal Ren-2 expression. In the DBA/2 inbred mouse strain expression in the female adrenal gland cycles during oestrus between the X-zone and the zona fasciculata. Transgene expression within the adrenal gland was restricted to the X-zone. Therefore this phenotype, which is characteristic of most two-renin-gene strains of mice, contrasts with that found in the strain DBA/2 from which the transgene was derived. This suggests that cell-specific expression in the DBA/2 adrenal gland is mediated in trans by at least one additional locus. We demonstrate that suitable genetic crosses of the transgenic mice can partially restore the cycling phenotype.
Mullins
J J
JJ
Department of Molecular and Cellular Biology, Roswell Park Memorial Institute, Buffalo, NY 14263.
Sigmund
C D
CD
Kane-Haas
C
C
Gross
K W
KW
McGowan
R A
RA
D000311:Adrenal Glands / Q000201:enzymology*; D000818:Animals; D015139:Blotting, Southern; D004247:DNA / Q000235:genetics; D004971:Estrus; D005260:Female; D015870:Gene Expression; D005796:Genes; D007668:Kidney / Q000201:enzymology; D051379:Mice; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D009693:Nucleic Acid Hybridization; D009928:Organ Specificity; D012313:RNA / Q000235:genetics; D015347:RNA Probes; D012083:Renin / Q000235:genetics*
D015347:RNA Probes; D012313:RNA; D004247:DNA; D012083:Renin