Atrial natriuretic peptide inhibits a cation channel in renal inner medullary collecting duct cells.
The patch-clamp technique was used to examine the effects of atrial natriuretic peptide (ANP) and its second messenger guanosine 3',5'-monophosphate (cGMP) on an amiloride-sensitive cation channel in the apical membrane of renal inner medullary collecting duct cells. Both ANP (10(-11) M) and dibutyryl guanosine 3',5'-monophosphate (10(-4) M) inhibited the channel in cell-attached patches, and cGMP (10(-5) M) inhibited the channel in inside-out patches. The inner medullary collecting duct is the first tissue in which ANP, via its second messenger cGMP, has been shown to regulate single ion channels. The results suggest that the natriuretic action of ANP is related in part to cGMP-mediated inhibition of electrogenic Na+ absorption by the inner medullary collecting duct.
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Department of Physiology, Dartmouth Medical School, Hanover, NH 03756.
Schwiebert
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Karlson
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Stanton
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D000634:Aminoquinolines / Q000494:pharmacology; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D002478:Cells, Cultured; D006152:Cyclic GMP / Q000494:pharmacology; D007473:Ion Channels / Q000187:drug effects*; D007679:Kidney Medulla / Q000187:drug effects; D007684:Kidney Tubules / Q000187:drug effects*; D007685:Kidney Tubules, Collecting / Q000187:drug effects*; D009318:Natriuresis; D051381:Rats; D012964:Sodium / Q000378:metabolism
D000634:Aminoquinolines; D007473:Ion Channels; D009320:Atrial Natriuretic Factor; C041715:6-anilino-5,8-quinolinedione; D012964:Sodium; D006152:Cyclic GMP