Cloning of the cDNA from normal brain and brain of patients with Alzheimer's disease in the expression vector lambda GT 11.
1. RNA was purified from postmortem human brains, and the poly A+ RNA was isolated by oligo dT cellulose. 2. Double stranded cDNA was synthesized using reverse transcriptase, RNAse H and DNA polymerase. 3. cDNA was cloned in the lambda GT 11 expression vector, and libraries containing between 1 and 2 millions clones were obtained. 92 to 98% of the plaques contained a recombinant phage. 4. Such libraries will allow the molecular characterization of cDNA and corresponding proteins which play a key role in brain functions and in particular which could be involved in the etiology of Alzheimer's dementia.
Octave
J N
JN
Université Catholique de Louvain, Laboratoire de Neurochimie, Brussels, Belgium.
de Sauvage
F
F
Macq
A F
AF
Maloteaux
J M
JM
D000544:Alzheimer Disease / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics*; D001921:Brain / Q000378:metabolism*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D005822:Genetic Vectors; D006801:Humans; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012016:Reference Values
D012313:RNA; D004247:DNA