The silver-stained proteins of interphasic nucleolar organizer regions as a parameter of cell duplication rate.
The relationship between interphasic silver-stained proteins of the nucleolar organizer regions (Ag-NOR proteins) and cell replication rate has been studied in 13 established neuroblastoma cell lines. The quantity of Ag-NOR proteins was measured in silver-stained cells by means of an automated image analyzer. The results indicated that the amount of Ag-NOR proteins is strictly proportional to the proliferative activity of the cells. Cell lines with a difference of only 4 h in doubling time were characterized by a statistically significant difference in Ag-NOR protein amount. The Ag-NOR protein quantity can therefore be used as a parameter for evaluating the cell proliferation rate.
Trerè
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Clinica Pediatrica III, University of Bologna, Italy.
Pession
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Derenzini
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D002455:Cell Division; D006801:Humans; D007399:Interphase; D009447:Neuroblastoma / Q000473:pathology*; D009687:Nuclear Proteins / Q000502:physiology*; D009697:Nucleolus Organizer Region / Q000502:physiology*; D012834:Silver; D013194:Staining and Labeling; D014407:Tumor Cells, Cultured
D009687:Nuclear Proteins; D012834:Silver