title: Synthesis of a more stable osmium ammine electron-dense DNA stain.
abstract: Specific DNA staining for electron microscopic observation is simplified by a shorter synthesis of the staining reagent. The new, more reliable reagent, osmium ammine-B, is stable for more than a year, dissolves completely in water, and does not require reoptimization of staining conditions for every batch, yet reproducibly gives strong contrast to DNA-containing structures.
authors: University of Tennessee-Oak Ridge, Graduate School of Biomedical Sciences.
authors: A L
authors: AL
authors: Olins
authors: B A
authors: BA
authors: Moyer
authors: S H
authors: SH
authors: Kim
authors: D P
authors: DP
authors: Allison
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D004247:DNA / Q000032:analysis*; D004355:Drug Stability; D008099:Liver / Q000032:analysis; D008854:Microscopy, Electron; D009992:Osmium / Q000138:chemical synthesis*; D017892:Osmium Compounds; D000644:Quaternary Ammonium Compounds / Q000138:chemical synthesis*; D051381:Rats; D012995:Solubility; D013194:Staining and Labeling; D014867:Water
chemical_list: D017892:Osmium Compounds; D000644:Quaternary Ammonium Compounds; C058639:osmium ammine B; D014867:Water; D009992:Osmium; D004247:DNA