Human interleukin 4 down-regulates the surface expression of CD5 on normal and leukemic B cells.
CD5 is a pan-T cell antigen which is expressed on a minor subset of normal B lymphocytes and on most chronic lymphocytic leukemia B cells (B-CLL). In this study, it is demonstrated that interleukin (IL) 4 down-regulates the surface expression of CD5 on tonsil B cells and B-CLL cells, but not on T cells. IL 4 inhibits both the spontaneous and the phorbol myristate acetate (PMA)-induced hyperexpression of CD5 on tonsil B cells. In contrast, IL 4 only suppresses the PMA-induced hyperexpression of CD5 on B-CLL, whereas the spontaneous CD5 expression is essentially unaffected. The IL 4 concentrations required to down-regulate CD5 are the same as those required to up-regulate CD23. The IL 4-mediated down-regulation of CD5 is an intrinsic property of this IL, since an anti-IL 4-neutralizing antiserum blocks this effect. Interferon (IFN)-gamma, which inhibits the IL 4 induced CD23 expression, does not block the IL 4-induced down-regulation of CD5. Recombinant IL 1 alpha, IL 2, IL 3, IL 5, IL 6, tumor necrosis factor (TNF)-alpha, IFN-gamma and granulocyte macrophage colony-stimulating factor neither inhibit nor stimulate CD5 expression on B cells, therefore suggesting that the capacity to down-regulate CD5 expression on B cells is specific to IL 4.
Defrance
T
T
UNICET, Laboratory for Immunological Research, Dardilly, France.
Vanbervliet
B
B
Durand
I
I
Banchereau
J
J
D000943:Antigens, Differentiation / Q000032:analysis / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D018956:CD5 Antigens; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology; D015847:Interleukin-4; D007378:Interleukins / Q000276:immunology / Q000494:pharmacology*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000187:drug effects; D014066:Palatine Tonsil; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism
D000943:Antigens, Differentiation; D018956:CD5 Antigens; D007106:Immune Sera; D007378:Interleukins; D015847:Interleukin-4; D007371:Interferon-gamma