A monoclonal antibody against the nuclear pore complex inhibits nucleocytoplasmic transport of protein and RNA in vivo.
A monoclonal antibody that reacts with proteins in the nuclear pore complex of rat liver (Snow, C. M., A. Senior, and L. Gerace. 1987. J. Cell Biol. 104:1143-1156) has been shown to cross react with similar components in Xenopus oocytes, as determined by immunofluorescence microscopy and immunoblotting. We have microinjected the antibody into oocytes to study the possible role of these polypeptides in nucleocytoplasmic transport. The antibody inhibits import of a large nuclear protein, nucleoplasmin, in a time- and concentration-dependent manner. It also inhibits export of 5S ribosomal RNA and mature tRNA, but has no effect on transcription or intranuclear tRNA processing. The antibody does not affect the rate of diffusion into the nucleus of two small proteins, myoglobin and ovalbumin, indicating that antibody binding does not result in occlusion of the channel for diffusion. This suggests that inhibition of protein and RNA transport occurs by binding of the antibody at or near components of the pore that participate in mediated transport.
Featherstone
C
C
Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Darby
M K
MK
Gerace
L
L
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001692:Biological Transport; D015153:Blotting, Western; D004058:Diffusion; D005455:Fluorescent Antibody Technique; D007158:Immunologic Techniques; D009685:Nuclear Envelope / Q000276:immunology* / Q000502:physiology*; D009865:Oocytes; D011506:Proteins / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*; D014982:Xenopus laevis
D000911:Antibodies, Monoclonal; D011506:Proteins; D012313:RNA