A model to induce scattered cell damage in the corneal endothelium.
A model to induce scattered cell damage in the corneal endothelium was set up by shaking a pig cornea in a glass bottle with 0.18% NaCl. Disseminated endothelial-cell losses, whose amounts correlated with the durations of treatment, were observed in the corneas which were shaken for less than 8 min. Following organ culturing for 1 week, most of the corneas still possessed intact endothelia which means that even after the treatment the endothelium still keeps a normal regenerative ability. With this method of design, a good model is provided to study the regeneration of the corneal endothelium.
Lin
C P
CP
Department of Ophthalmology, Kaohsiung Medical College, Taiwan, ROC.
Böhnke
M
M
Draeger
J
J
D000818:Animals; D002452:Cell Count; D002470:Cell Survival; D004728:Endothelium, Corneal / Q000293:injuries*; D008954:Models, Biological; D009924:Organ Culture Techniques; D009994:Osmolar Concentration; D012038:Regeneration; D013194:Staining and Labeling; D013552:Swine; D013997:Time Factors