Nucleoside uptake by Trichomonas vaginalis.
A rapid sampling technique was used to follow nucleoside uptake by Trichomonas vaginalis. The results indicated that nucleoside uptake is biphasic with time. Adenosine, guanosine, and uridine uptake is carrier mediated, transported substrate is rapidly metabolised to nucleotides. Two separate carriers appear to exist, one which transports all nucleosides and a second which transports adenosine, guanosine and uridine. Both carriers have more than one binding site for nucleosides. The first carrier has sites for adenosine and pyrimidine nucleosides, and a separate site for purine nucleosides. The second carrier has a site for adenosine and uridine and a separate site for guanosine. Adenosine uptake could not be completely inhibited by nitrobenzylthionucleosides. The rate of nucleoside uptake by T. vaginalis is sufficient to sustain growth.
Harris
D I
DI
Department of Biochemistry, University College of Wales, Aberystwyth, U.K.
Beechey
R B
RB
Linstead
D
D
Barrett
J
J
D000241:Adenosine / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D001667:Binding, Competitive; D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism; D003562:Cytidine / Q000494:pharmacology; D004247:DNA / Q000032:analysis; D004109:Dilazep / Q000494:pharmacology; D006151:Guanosine / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D007288:Inosine / Q000494:pharmacology; D007700:Kinetics; D009705:Nucleosides / Q000378:metabolism*; D012313:RNA / Q000032:analysis; D013868:Thioinosine / Q000031:analogs & derivatives / Q000494:pharmacology; D013872:Thionucleosides / Q000494:pharmacology; D013936:Thymidine / Q000494:pharmacology; D014246:Trichomonas vaginalis / Q000235:genetics / Q000378:metabolism*; D014529:Uridine / Q000378:metabolism / Q000494:pharmacology
D002352:Carrier Proteins; D009705:Nucleosides; D013872:Thionucleosides; D006151:Guanosine; C002121:6-(4-nitrobenzylthio)guanosine; D013868:Thioinosine; D007288:Inosine; D003562:Cytidine; D012313:RNA; D004247:DNA; D004109:Dilazep; C001789:4-nitrobenzylthioinosine; D000241:Adenosine; D013936:Thymidine; D014529:Uridine