[Plasma cholecystokinin concentration in patients with chronic pancreatitis measured by bioassay].
We developed a specific and sensitive bioassay for measuring plasma cholecystokinin (CCK) in human and investigated CCK response after a test meal in patients with chronic pancreatitis. Treatment with cycloheximide increased the sensitivity and responsiveness of isolated rat pancreatic acini to CCK-octapeptide (CCK-8) and thus plasma levels of CCK-8 as low was 0.17 pM were detectable. Fasting plasma CCK levels in normal subjects as CCK-8 equivalents were 0.75 +/- 0.25 pM and rose to a peak of 6.2 +/- 0.68 pM at 45 min after a test meal consisting of 400 ml milk and 2 boiled eggs. Basal and stimulated plasma levels of CCK-8 in patients with non-calcified chronic pancreatitis were significantly higher than those in normal subjects. In contrast, postprandial responses of plasma CCK-8 in patients with calcified chronic pancreatitis was significantly low compared to those in control subjects, although basal plasma levels were not significantly different from those in controls.
Koide
M
M
Okabayashi
Y
Y
Hasegawa
H
H
Tani
S
S
Fujisawa
T
T
Nakamura
T
T
Fujii
M
M
Otsuki
M
M
D000681:Amylases / Q000378:metabolism; D000818:Animals; D001681:Biological Assay / Q000379:methods; D002766:Cholecystokinin / Q000097:blood*; D002908:Chronic Disease; D006801:Humans; D066298:In Vitro Techniques; D010179:Pancreas / Q000378:metabolism; D010195:Pancreatitis / Q000097:blood*; D051381:Rats
D002766:Cholecystokinin; D000681:Amylases