Light microscopic immunocytochemical demonstration of thyroid-stimulating hormone (TSH) receptors on normal rat thyroid cells.
We present a method for immunohistochemical demonstration of surface-associated thyroid-stimulating hormone (TSH) receptors on direct imprints of rat thyroid follicular cells at the light microscopic level, by use of dinitrophenyl (DNP)-labeled bovine TSH as the primary probe in a DNP-hapten sandwich staining (DHSS) procedure. The light microscopically invisible diaminobenzidine (DAB) product of the enzyme reaction was amplified with a DAB enhancement technique (silver amplification), which yielded a reliable and distinct light microscopically visible cell surface staining. The consistently negative control results after inhibition with TSH receptor-specific reagents provide evidence in support of immunohistochemical localization of surface membrane-associated TSH receptors.
Schmid
K W
KW
Department of Pathology, University of Wales College of Medicine, Heath Park, Cardiff, United Kingdom.
Jasani
B
B
Morgan
J M
JM
Williams
E D
ED
D000818:Animals; D002417:Cattle; D004140:Dinitrophenols / Q000378:metabolism; D005260:Female; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D011989:Receptors, Thyrotropin / Q000032:analysis* / Q000378:metabolism; D012834:Silver; D013194:Staining and Labeling; D013961:Thyroid Gland / Q000032:analysis*; D013972:Thyrotropin / Q000378:metabolism
D004140:Dinitrophenols; D011989:Receptors, Thyrotropin; D012834:Silver; D013972:Thyrotropin