A monoclonal antibody that recognizes a cluster of a disaccharide, NeuAc alpha(2----6)GalNAc, in mucin-type glycoproteins.
The structure of an epitopic carbohydrate recognized by a monoclonal antibody, MLS 102, was determined. A disaccharide, NeuAc alpha (2----6)GalNAc, the major prosthetic group of ovine submaxillary mucin (OSM) and related synthetic glycosides, NeuAc alpha(2----6)GalNAc alpha----Ser, NeuAc alpha(2----6)GalNAc beta----Ser, and NeuAc alpha (2----6)GalNAc beta----propyl, reacted with MLS 102 to similar extents, but the reaction was considerably weaker compared to that of OSM. This difference in reactivity could be ascribed to the occurrence of a cluster of the disaccharide on OSM. Purification of MLS 102-reactive antigens from a Triton X-100 extract of LS 180 cells by means of immunoaffinity chromatography gave mucin fractions (cMLS 102 antigen) with an OSM-like domain. Correlation between the content of the disaccharide, NeuAc alpha(2----6)GalNAc, in mucins and their reactivity with MLS 102 was observed.
Kurosaka
A
A
Division of Biological Engineering, Kyoto Sangyo University, Japan.
Kitagawa
H
H
Fukui
S
S
Numata
Y
Y
Nakada
H
H
Funakoshi
I
I
Kawasaki
T
T
Ogawa
T
T
Iijima
H
H
Yamashina
I
I
D000936:Antigen-Antibody Complex / Q000032:analysis; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002460:Cell Line; D003110:Colonic Neoplasms; D004187:Disaccharides / Q000276:immunology*; D000939:Epitopes / Q000032:analysis*; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D009077:Mucins / Q000276:immunology / Q000302:isolation & purification
D000936:Antigen-Antibody Complex; D004187:Disaccharides; D000939:Epitopes; D006023:Glycoproteins; D009077:Mucins