title: Cultured liver epithelial cells can incorporate monoclonal antibodies directed against intracellular cytokeratin structures without microinjection.
abstract: T51B rat liver cells in the exponential phase of growth were arrested in late G1 by medium calcium deprivation, exposed to mouse monoclonal anti-cytokeratin (Mr = 55,000) by addition of the antibody to the medium, induced to enter the S phase by readdition of 1.5 mM calcium, and incubated for a further 18 h. After fixing and staining with FITC-conjugated anti-mouse IgG, the cells exhibited an intact intracellular cytokeratin-staining pattern. No effect of antibody was observed on entry of cells into the S phase. These results show that rat liver-derived epithelial cells can actively take up macromolecules like cytokeratin antibodies without microinjection.
authors: Health and Welfare Canada, Drug Toxicology Division, Ottawa.
authors: S H
authors: SH
authors: Swierenga
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002453:Cell Cycle; D002478:Cells, Cultured; D004848:Epithelium / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D007382:Intermediate Filaments / Q000502:physiology; D007633:Keratins / Q000276:immunology / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D051381:Rats
chemical_list: D000911:Antibodies, Monoclonal; D007633:Keratins