Antigenic determinants resulting from polymerization of immunoglobulin G.
Antibodies against heat-aggregated human IgG (AHG) were raised in rabbits and those against immune complex (IC) of tetanus toxoid and its human antitoxin (T/aT) in rabbits and mice. These antisera were analyzed by the Ouchterlony double diffusion technique against native monomeric human IgG (NHG), AHG and chemically linked human IgG (poly-G). The appearance of neoantigens was demonstrated with human IgG bound to its corresponding antigen or denaturated nonspecifically. The results are indicative of the existence of a common determinant shared with AHG and T/aT, in addition to those of their own.
Arai
K
K
Central Laboratory for Clinical Investigation, Osaka University Medical School, Japan.
Yasuda
J
J
Sasaki
T
T
Yonemasu
K
K
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000936:Antigen-Antibody Complex / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D006801:Humans; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000276:immunology*; D051379:Mice; D011108:Polymers / Q000276:immunology; D011817:Rabbits; D013743:Tetanus Antitoxin / Q000276:immunology; D013745:Tetanus Toxoid / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000936:Antigen-Antibody Complex; D000939:Epitopes; D007074:Immunoglobulin G; D011108:Polymers; D013743:Tetanus Antitoxin; D013745:Tetanus Toxoid