Enzyme-linked immunosorbent assay for alpha-fetoprotein in human serum.
A simple and inexpensive enzyme-linked immunosorbent assay for determination of serum alpha-fetoprotein concentration, with results available within 4 h, is described. The assay has a working range of 2-100 ng/ml, with within- and between-batch coefficients of variation (n = 24) at different concentrations between 8.9 and 12.7%. Results on 80 patients' sera (AFP concentrations 0-175 ng/ml) correlate well with those by radioimmunoassay. Turn around time (4 h) is shorter than with similar immunoassays currently available.
Chui
S H
SH
Yow
C M
CM
Lam
C W
CW
Wan
K C
KC
Lewis
W H
WH
D006528:Carcinoma, Hepatocellular / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood*; D011786:Quality Control; D012016:Reference Values; D000509:alpha-Fetoproteins / Q000032:analysis*
D000509:alpha-Fetoproteins