title: Diagnostic Mr31/32,000 Schistosoma mansoni proteins (Sm31/32): reaction with sera from Sudanese patients infected with S. mansoni or S. haematobium.
abstract: Sera of Sudanese patients with active infections of Schistosoma mansoni or S. haematobium were tested in immunoblots for their reactivity with Mr31/32,000 proteins of adult S. mansoni (Sm31/32). All sera from patients with intestinal (n = 123) and all but one from those with urinary schistosomiasis (n = 35) had antibodies against Sm31/32. These and additional data suggest that both specificity and sensitivity of Sm31/32 to detect schistosome infections are close to 100%. Antibodies against these proteins developed also in monkeys after experimental infection with S. haematobium. Sm31/32 antigens reacted in immunoblots as a doublet with most S. haematobium-patient sera and as a broad band with many S. mansoni-sera suggesting that at least two components are present in the molecular weight region of Mr31/32,000. The data demonstrate the potential use of Sm31/32 from adult worms to diagnose patients with intestinal or urinary schistosomiasis in endemic areas.
authors: Institute for Tropical Hygiene, University of Heidelberg, Federal Republic of Germany.
authors: M A
authors: MA
authors: Idris
authors: A
authors: A
authors: Ruppel
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis; D000947:Antigens, Helminth / Q000276:immunology*; D002648:Child; D003429:Cross Reactions; D000939:Epitopes; D004901:Erythrocebus patas; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008970:Molecular Weight; D010270:Parasite Egg Count; D011237:Predictive Value of Tests; D012553:Schistosomiasis haematobia / Q000175:diagnosis*; D012555:Schistosomiasis mansoni / Q000175:diagnosis*; D012698:Serologic Tests; D013397:Sudan
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D000939:Epitopes