Effects of type I and II interferons on cultured human breast cells: interaction with estrogen receptors and with tamoxifen.
The combined effects of tamoxifen, a competitive inhibitor of estrogen, and type I or II interferons on the proliferation of several human breast cancer cell lines in vitro were examined. Additive antiproliferative effects were observed with interferons and tamoxifen, in two estrogen receptor positive cell lines, MCF-7 and T-47D. In MCF-7 cells neither beta ser interferon, gamma interferon, nor alpha 6 interferon were able to significantly alter estrogen receptor levels. Antigrowth activities of beta ser interferon and gamma interferon in an estrogen receptor negative cell line, HS578T, were equivalent to those in estrogen receptor-positive cell lines. Consistent with the antiproliferative effects of interferons, high affinity beta ser interferon receptors and interferon inducible 2'5'-oligoadenylate synthetase were present in both MCF-7 and HS578T cell lines. Thus steroid hormone receptor status did not influence the antiproliferative effects of interferons on breast carcinoma cells in vitro.
Goldstein
D
D
Department of Human Oncology, University of Wisconsin Cancer Center, Madison 53792.
Bushmeyer
S M
SM
Witt
P L
PL
Jordan
V C
VC
Borden
E C
EC
D015088:2',5'-Oligoadenylate Synthetase / Q000096:biosynthesis; D001943:Breast Neoplasms / Q000032:analysis / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D004357:Drug Synergism; D005260:Female; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology; D000068556:Interferon beta-1a; D000068576:Interferon beta-1b; D016899:Interferon-beta; D007372:Interferons / Q000494:pharmacology*; D011960:Receptors, Estrogen / Q000032:analysis* / Q000187:drug effects; D011971:Receptors, Immunologic / Q000032:analysis; D017471:Receptors, Interferon; D011994:Recombinant Proteins / Q000494:pharmacology; D013629:Tamoxifen / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D007370:Interferon Type I; D011960:Receptors, Estrogen; D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D011994:Recombinant Proteins; D013629:Tamoxifen; D000068576:Interferon beta-1b; D016899:Interferon-beta; D007372:Interferons; D015088:2',5'-Oligoadenylate Synthetase; D000068556:Interferon beta-1a