Mutagen-induced chromosome fragility within peripheral blood lymphocytes of head and neck cancer patients.
The expression of chromosome breakage as a response to mutagen exposure may contribute to the development of head and neck cancer. Lymphocytes from 46 previously untreated head and neck cancer patients were cultured in vitro and exposed to the radiomimetic clastogen, bleomycin. The lymphocytes were then arrested in metaphase and analyzed for bleomycin-induced chromosome breaks. Mean bleomycin-induced chromosome breaks per cell (b/c) in head and neck cancer patients (0.94 +/- 0.3 b/c) were significantly higher than in either controls (0.70 +/- 0.3 b/c; p less than 0.001) or a concurrently examined patient population with central nervous system tumors (0.55 +/- 0.3 b/c; p less than 0.001). The expression of mutagen-induced chromosome fragility in the head and neck cancer population was site-specific, being most evident in patients with laryngeal or pharyngeal cancer but not oral cavity disease. The differential responses to mutagen effects may be a reflection of DNA repair deficiency. Head and neck cancer patients express a higher degree of chromosomal mutagen sensitivity, which may contribute to neoplastic development.
Schantz
S P
SP
Department of Head and Neck Surgery, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Hsu
T C
TC
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001761:Bleomycin / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000235:genetics; D002873:Chromosome Fragility; D005260:Female; D006258:Head and Neck Neoplasms / Q000097:blood / Q000235:genetics*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics; D009153:Mutagens / Q000009:adverse effects*; D010610:Pharyngeal Neoplasms / Q000235:genetics; D014029:Tobacco Use Disorder / Q000235:genetics
D009153:Mutagens; D001761:Bleomycin