[Effect of ultraviolet irradiation of autologous blood on cell volumes, cell adhesion and phagocytosis in normal probands and patients with multiple sclerosis].
UVB induced changes of blood cell properties were investigated in 12 MS patients and in 10 healthy volunteers serving as normal controls. The mean cell volume (MCV) was determined by electronic sizing, the granulocyte and lymphocyte adherence was estimated in a capillary assay, and the phagocytic activity of granulocytes was measured in a test system based on the incorporation of opsonized baker's yeast (Saccharomyces cerevisiae). In MS patients the MCV of red cells and lymphocytes decreased rapidly within 6 UVB treatments. In contrast, the reduction of the granulocyte volume was delayed (between the 6th and 12th UVB). In the control group the mean value of the red cell and lymphocyte MCV remained rather unaffected. There was a slight rise of the granulocyte volume after the 6th UVB. The only significant change of adherence was an increase of granulocyte adherence in MS patients. Untreated patients had a significantly enhanced phagocytic activity in comparison to the control group. 6 UVB treatments included a significant reduction of the phagocytic activity in MS patients. However, subsequently the percentage of phagocytizing cells increased again, whereas the particle uptake per cell continued to decrease. In the control group only minor UVB induced changes of phagocytosis were observed. The in vitro UV irradiation caused an enhanced phagocytosis in the majority of cases in both controls and MS patients. In general, under the UVB treatment all parameters examined changed in the sense of a normalisation, in that the measured values reached a new level lying between the extreme pretreatment values accompanied by a reduced standard deviation. The effect of UVB was more pronounced in MS patients when compared with normal controls. This could result from an enhanced sensitivity to the influence of UVB of pathologically altered cells in MS patients. The monitoring of the MCV of red cells and lymphocytes as well as the repeated testing of granulocyte phagocytosis are recommended for supportion of therapy planning and follow-up of MS patients.
Mix
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Forschungsgruppe Neuroimmunologie, Bereiches Medizin der Wilhelm-Pieck-Universität, Rostock.
Jenssen
H L
HL
Lehmitz
R
R
Buddenhagen
F
F
Hitzschke
B
B
Richter
M
M
D001769:Blood / Q000528:radiation effects*; D001772:Blood Cell Count / Q000528:radiation effects*; D001804:Blood Transfusion, Autologous; D002448:Cell Adhesion / Q000528:radiation effects*; D004912:Erythrocytes / Q000528:radiation effects; D006098:Granulocytes / Q000528:radiation effects; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects; D009103:Multiple Sclerosis / Q000097:blood / Q000532:radiotherapy*; D010587:Phagocytosis / Q000528:radiation effects*
Einfluss von Ultraviolettbestrahlung des Blutes (UVB) auf Zellvolumen, Zelladhärenz und Phagozytose bei Normalprobanden und Patienten mit Multipler Sklerose (MS).