title: An exonuclease possibly involved in the initiation of repair of bleomycin-damaged DNA in mouse ascites sarcoma cells.
abstract: A protein factor having exonucleolytic activity on bleomycin-damaged DNA and providing priming sites for DNA polymerases existed in a DNA polymerase beta fraction partially purified by ion exchange chromatography from an extract of permeable mouse ascites sarcoma (SR-C3H/He) cells. The exonuclease was separated from DNA polymerase beta by single-stranded DNA-cellulose chromatography, and partially characterized. The enzyme is suggested to be involved in the initial step of repair of bleomycin-damaged DNA in removing 3' ends (3'-phosphoglycolate termini) of bleomycin-damaged DNA.
authors: Department of Biochemistry, Okayama University Medical School, Japan.
authors: S
authors: S
authors: Seki
authors: T
authors: T
authors: Oda
mesh_terms: D000818:Animals; D001761:Bleomycin / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects; D004249:DNA Damage; D004256:DNA Polymerase I / Q000378:metabolism; D004260:DNA Repair; D005092:Exonucleases / Q000502:physiology*; D007501:Iron / Q000494:pharmacology; D051379:Mice; D012513:Sarcoma, Experimental / Q000378:metabolism; D013698:Templates, Genetic
chemical_list: D001761:Bleomycin; D004247:DNA; D007501:Iron; D004256:DNA Polymerase I; D005092:Exonucleases