title: Interaction of the Enhancer of white-apricot with transposable element alleles at the white locus in Drosophila melanogaster.
abstract: The Enhancer of wa [E(wa)] mutation was shown to interact strongly with 4 of 41 tested alleles of the white (w) eye color locus. All four of the affected w alleles result from the insertion of a transposable element. E(wa) was further localized cytogenetically. The locus lies between the breakpoints of T(Y;2)L11 and T(Y;2)H137 (section 60) in 2R. The original mutation was shown to be antimorphic on the basis of its action in the presence of additional normal copies and the ability to revert the original allele to one that mimics the effect of a deficiency for the locus. The RNA transcribed from wa was analyzed from flies segregating for E(wa) and normal. The low level of normal functional messenger RNA present in white-apricot is reduced further in Enhancer homozygotes. Total copia RNA was also examined on Northern analyses from the segregating population but no quantitative change in the major copia RNA was produced by E(wa) homozygotes compared to normal.
authors: Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138.
authors: J A
authors: JA
authors: Birchler
authors: J C
authors: JC
authors: Hiebert
mesh_terms: D000483:Alleles; D000818:Animals; D015152:Blotting, Northern; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D005020:Ethyl Methanesulfonate; D006720:Homozygote; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D012313:RNA / Q000032:analysis / Q000302:isolation & purification; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis; D014178:Translocation, Genetic
chemical_list: D004251:DNA Transposable Elements; D015347:RNA Probes; D012333:RNA, Messenger; D012313:RNA; D005020:Ethyl Methanesulfonate