Monoclonal antibodies specific for murine CD2 reveal its presence on B as well as T cells.
Monoclonal antibodies specific for the murine CD2 antigen were identified by an efficient screening method utilizing murine CD2 cDNA transfectants. An unexpected expression of CD2 on murine B cells was revealed by immunofluorescence and immunoprecipitation studies with these monoclonal antibodies and by RNA blot analysis for the murine CD2 transcript.
Yagita
H
H
Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan.
Nakamura
T
T
Karasuyama
H
H
Okumura
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis* / Q000235:genetics; D000954:Antigens, Surface / Q000032:analysis / Q000235:genetics; D001402:B-Lymphocytes / Q000276:immunology*; D015152:Blotting, Northern; D018801:CD2 Antigens; D002460:Cell Line; D006224:Cricetinae; D004247:DNA / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D007106:Immune Sera / Q000276:immunology; D011233:Precipitin Tests; D012313:RNA / Q000032:analysis; D051381:Rats; D011971:Receptors, Immunologic / Q000032:analysis* / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology*; D014162:Transfection
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D018801:CD2 Antigens; D007106:Immune Sera; D011971:Receptors, Immunologic; D012313:RNA; D004247:DNA