title: Antigenic sites in carcinoembryonic antigen.
abstract: The epitope reactivities of 52 well-characterized monoclonal antibodies (Mabs) against carcinoembryonic antigen from 11 different research groups were studied using competitive solid-phase immunoassays. About 60% of all possible combinations of Mabs as inhibitors and as the primary binding antibody were investigated. The inhibition data were analyzed by a specially developed computer program "EPITOPES" which measures concordance and discordance in inhibition patterns between Mabs. The analysis showed that 43 of the 52 Mabs (83%) could be classified into one of five essentially noninteracting epitope groups (GOLD 1-5) containing between four and 15 Mabs each. The epitopes recognized by the Mabs belonging to groups 1 to 5 were peptide in nature. With one or two possible exceptions non-classifiable Mabs were either directed against carbohydrate epitopes (4 Mabs) or were inactive in the tests used. Within epitope groups GOLD 1, 4, and 5 two partially overlapping subgroups were distinguished. Mabs with a high degree of carcinoembryonic antigen specificity generally belonged to epitope groups GOLD 1 and 3.
authors: University of Umeå.
authors: S
authors: S
authors: Hammarstrom
authors: J E
authors: JE
authors: Shively
authors: R J
authors: RJ
authors: Paxton
authors: B G
authors: BG
authors: Beatty
authors: A
authors: A
authors: Larsson
authors: R
authors: R
authors: Ghosh
authors: O
authors: O
authors: Bormer
authors: F
authors: F
authors: Buchegger
authors: J P
authors: JP
authors: Mach
authors: P
authors: P
authors: Burtin
mesh_terms: D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D007158:Immunologic Techniques; D012984:Software
chemical_list: D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D000939:Epitopes