Characterization of Sicilian strains of spotted fever group rickettsiae by using monoclonal antibodies.
Twenty-two hybridomas producing anti-Rickettsia conorii monoclonal antibodies were obtained by nine fusion experiments. The strain chosen for immunization of mice was MAVI, an R. conorii strain isolated from a Sicilian patient with Boutonneuse fever. When tested for immunoglobulin isotype by an indirect immunofluorescence (IIF) assay, 46.6% of supernatants from the 22 hybridomas were immunoglobulin M. The supernatants were tested in the IIF assay for binding to the MAVI strain and four spotted fever group rickettsia strains isolated from Sicilian ticks (two virulent and two nonpathogenic when inoculated intraperitoneally in male guinea pigs). Only five of the supernatants showed a positive IIF result on all tested strains, although they produced different titers to the various strains, possibly an indication that they recognized an antigen common to spotted fever group rickettsiae. Immunodominant epitopes for humans were determined by using patient sera to analyze inhibition of binding to the MAVI strain. Although a limited number of serum samples were screened, a high percentage of Boutonneuse fever patients produced antibodies recognizing the same epitopes as were recognized by the mouse monoclonal antibodies. A striking heterogeneity was found both in the expression of mouse-recognized epitopes on the five rickettsial strains and in the serum antibody responses of Boutonneuse fever patients to these epitopes.
Vitale
G
G
Cattedra di Patologia Speciale Medica e Metodologia Clinica, Piazza delle Cliniche, Palermo, Italy.
Di Stefano
R
R
Damiani
G
G
Mansueto
S
S
D000328:Adult; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000940:Antigenic Variation; D000942:Antigens, Bacterial / Q000032:analysis*; D001907:Boutonneuse Fever / Q000382:microbiology*; D000939:Epitopes / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006801:Humans; D006825:Hybridomas; D007136:Immunoglobulins / Q000032:analysis / Q000276:immunology; D008297:Male; D051379:Mice; D012281:Rickettsia / Q000276:immunology*; D013987:Ticks
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes; D007136:Immunoglobulins