Biocytin hydrazide--a selective label for sialic acids, galactose, and other sugars in glycoconjugates using avidin-biotin technology.
Biocytin hydrazide (BCHZ), a new, water-soluble, long-chained, biotin-containing hydrazide, was synthesized and used for the selective nonradioactive detection of glycoconjugates. Procedures were developed for labeling glycoconjugates on blots. The method involves either chemical (periodate-induced) or enzymatic (via galactose oxidase) oxidation of glycoconjugates, the resultant aldehyde groups are then labeled with biocytin hydrazide, followed by interaction with an avidin-based enzyme probe. Since the biotin-containing reagent is a relatively small, charged molecule, the primary labeling step may be carried out on intact cells and on membrane preparations as well as on blotted samples. On blots, the labeling pattern was similar for both periodate- and galactose oxidase-induced biotinylation procedures. In contrast, periodate-induced labeling of either erythrocyte membranes or cells (prior to blotting) produced an altered labeling pattern. Combined enzyme-induced biotinylation of membranes or cells resulted in a pattern similar to that observed for the direct staining of blots. Using galactose oxidase on human erythrocyte membranes, the procedure was sensitive enough to selectively label the Band 3 lactosaminoglycoprotein.
Bayer
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EA
Department of Biophysics, Weizmann Institute of Science, Rehovot, Israel.
Ben-Hur
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H
Wilchek
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D001360:Avidin / Q000097:blood; D001798:Blood Proteins / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis*; D004910:Erythrocyte Membrane / Q000032:analysis; D005690:Galactose / Q000032:analysis*; D006001:Glycoconjugates / Q000032:analysis*; D006023:Glycoproteins; D006801:Humans; D006834:Hydrazines; D008239:Lysine / Q000031:analogs & derivatives*; D008565:Membrane Proteins / Q000032:analysis; D012794:Sialic Acids / Q000032:analysis*; D013194:Staining and Labeling; D000093422:Glycated Serum Proteins
D001798:Blood Proteins; D002241:Carbohydrates; D006001:Glycoconjugates; D006023:Glycoproteins; D006834:Hydrazines; D008565:Membrane Proteins; D012794:Sialic Acids; C048725:biocytin hydrazide; D001360:Avidin; D008239:Lysine; D005690:Galactose; D000093422:Glycated Serum Proteins