A new staining and evaluating procedure for protein gel electropherograms based on the pyrogallol red-molybdate complex.
A new method is reported for staining and evaluating gel electropherograms of proteins. With pyrogallol red-molybdate reagent the gel-embedded proteins are transformed into a derivative of blue colour. After destaining, the blue-coloured proteins are well visible against a colourless background and can be quantified by densitometry with high reliability. The quantity of the coloured protein is directly proportional to the height of peaks in the densitogram. Colour intensity is concentration dependent. The measurement range of serum albumin was 1 to 50 micrograms/tube and 10 to 100 micrograms/slab in polyacrylamide gel disc electrophoresis and agar gel electrophoresis, respectively.
Csiba
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A
Szécsényi-Nagy
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L
D004586:Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D008982:Molybdenum; D011506:Proteins / Q000032:analysis*; D011748:Pyrogallol / Q000031:analogs & derivatives*; D012709:Serum Albumin / Q000032:analysis; D013194:Staining and Labeling
D011506:Proteins; D012709:Serum Albumin; D011748:Pyrogallol; C044659:molybdate; C058731:pyrogallol sulfonphthalein; D008982:Molybdenum