Electrophoretic location of epitopes on human transferrin.
Competitive assays of antigen--antibody interaction using solid-bound antigen or antibodies can be affected by steric restrictions. A suitable electrophoretic system may be used to follow the interactions in solution. Electrophoresis of human transferrin complexed with pairs of monoclonal antibodies raised against it revealed three types of interaction: competition for closely neighbouring epitopes, simultaneous binding to distinct epitopes, and a type of interaction suggesting formation of multiply linked antigen--antibody complexes.
Slavícková
A
A
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D004586:Electrophoresis; D000939:Epitopes / Q000032:analysis*; D006801:Humans; D014168:Transferrin / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D014168:Transferrin