Porin from Thiobacillus versutus.
The porin of Thiobacillus versutus IFO 14567 was isolated by extraction of cell-envelopes with sodium dodecyl sulfate. It exhibited strong porin-activity after reconstitution into artificial lipid bilayer membranes. The diameter of the pore was determined as 1.6 nm, with a weak selectivity for cations being observed. The porin migrated as a single band (Mr 35 kDa) on SDS-polyacrylamide gel-electrophoresis after heating (100 degrees C, 5 min). The porin oligomer was not sensitive towards EDTA. Analytical ultracentrifugation studies demonstrated the native oligomer to be a trimer.
Woitzik
D
D
Institut für Biologie II, Mikrobiologie, Albert Ludwigs Universität, Freiburg, F.R.G.
Benz
R
R
Lustig
A
A
Weckesser
J
J
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D007473:Ion Channels / Q000032:analysis / Q000378:metabolism*; D046911:Macromolecular Substances; D008970:Molecular Weight; D018272:Porins; D013855:Thiobacillus / Q000378:metabolism*; D014461:Ultracentrifugation
D001425:Bacterial Outer Membrane Proteins; D007473:Ion Channels; D046911:Macromolecular Substances; D018272:Porins; D004492:Edetic Acid