[The action of gamma irradiation on terrilytin immobilized on cellulose-fiber materials].
The effect of gamma-radiation on terrilytin, a proteolytic enzyme immobilized on modified and nonmodified cellulose materials was studied by EPR. Dialdehyde cellulose and graft copolymer of cellulose and polyacrylic acid were used as the modified cellulose materials. Dependence of the native and immobilized terrilytin activity and the content of free radicals in the irradiated samples on the irradiation dose was observed. It was shown that immobilization of the enzyme led to increasing of its stability to the effect of the ionizing radiation. This was due to transfer of the free valency from terrilytin to the carrying polymer which prevented radiation and chemical destruction of the enzyme. The proteolytic activity of native terrilytin subjected to gamma-irradiation markedly decreased because of intramolecular and intermolecular interactions during reactions of the terrilytin free radicals, since in this case there was no polymer as an acceptor of the enzyme free valency.
Iudanova
T N
TN
Skokova
I F
IF
Dovbiĭ
E V
EV
Maziĭ
G A
GA
Kalashnik
A T
AT
D000681:Amylases / Q000032:analysis / Q000528:radiation effects*; D000890:Anti-Infective Agents / Q000032:analysis / Q000528:radiation effects*; D001458:Bandages; D002482:Cellulose / Q000031:analogs & derivatives; D002483:Cellulose, Oxidized; D004307:Dose-Response Relationship, Radiation; D004337:Drug Carriers; D004338:Drug Combinations / Q000032:analysis / Q000528:radiation effects; D004578:Electron Spin Resonance Spectroscopy; D004795:Enzyme Stability / Q000528:radiation effects; D004800:Enzymes, Immobilized / Q000032:analysis / Q000528:radiation effects*; D005609:Free Radicals; D005720:Gamma Rays; D010447:Peptide Hydrolases / Q000032:analysis / Q000528:radiation effects*; D011854:Radiochemistry
D000890:Anti-Infective Agents; D002483:Cellulose, Oxidized; D004337:Drug Carriers; D004338:Drug Combinations; D004800:Enzymes, Immobilized; D005609:Free Radicals; C009402:amylase, phosphates, proteases drug combinations; D002482:Cellulose; D000681:Amylases; D010447:Peptide Hydrolases
Deĭstvie gamma-oblucheniia na terrilitin, immobilizovannyĭ na tselliuloznykh voloknistnykh materialakh.