[An analysis of DNA secondary structure during lymphocyte activation by mitogens].
The DNA structure of human peripheral blood lymphocytes was studied following activation by concanavalin A in vitro. DNA strand breaks were measured by the fluorometric method after DNA alkaline denaturation in cell lysates. Stimulation of lymphocytes proliferation was controlled by 3H-thymidine and 3H-uridine incorporation. It was shown that 1-3 hours after the addition of mitogens the quantity of alkali-labile sites in DNA increased. Cultivation of lymphocytes in vitro without mitogens was accompanied by gradual accumulation of alkali-labile sites in DNA and in 18-72 hours the DNA structure of lymphocytes in the culture was the same. It is possible that the rapid disturbances in the DNA secondary structure of lymphocytes following activation by mitogens is determined by the transmembrane signal transduction and is the one of the early events in genome activation.
Moskaleva
E Iu
EIu
D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects* / Q000235:genetics; D008934:Mitogens / Q000494:pharmacology*; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D009691:Nucleic Acid Denaturation / Q000187:drug effects / Q000235:genetics; D012313:RNA / Q000096:biosynthesis / Q000187:drug effects / Q000235:genetics; D013997:Time Factors
D008934:Mitogens; D012313:RNA; D004247:DNA
Analiz vtorichnoĭ struktury DNK pri aktivatsii limfotsitov mitogenami.