title: The chick chorioallantoic membrane assay: an improved technique for the study of angiogenic activity.
abstract: The chick chorioallantoic membrane assay has been used for many years as an in vivo assay of angiogenic activity. However it has been plagued with the problem of a high incidence of false positive angiogenic responses. In this study the conventional chick chorioallantoic membrane assay was compared with a modification of this technique in a series of batches using a total of 100 eggs. False positive reactions were recorded in 32 out of 50 (64%) using the conventional method and only 11 out of 50 (22%) with the modified method (chi 2 = 17.992; p less than 0.001).
authors: Department of Obstetrics and Gynaecology, University of Sydney, New South Wales, Australia.
authors: M J
authors: MJ
authors: Peek
authors: T M
authors: TM
authors: Norman
authors: C
authors: C
authors: Morgan
authors: R
authors: R
authors: Markham
authors: I S
authors: IS
authors: Fraser
mesh_terms: D000650:Amnion / Q000098:blood supply*; D000818:Animals; D002642:Chick Embryo; D002823:Chorion / Q000098:blood supply*; D005188:False Negative Reactions; D005189:False Positive Reactions; D008954:Models, Biological; D009389:Neovascularization, Pathologic