Analysis of brain and myelin lipids by high-performance thin layer chromatography and densitometry.
We have developed a simple method involving high-performance thin layer chromatographic separation of total brain and myelin lipids. Only two solvent systems consisting of chloroform: methanol:acetic acid and water at different concentrations were needed. The plate was then stained with three sequential procedures to visualize phospholipids, cholesterol and galactolipids. Densitometric procedure at each step of staining was utilized to obtain quantitative analysis of brain and myelin samples.
Di Biase
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Dept. Metabolism and Pathological Biochemistry, Istituto Superiore di Sanità, Rome, Italy.
Salvati
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D000818:Animals; D001923:Brain Chemistry; D002784:Cholesterol / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer / Q000379:methods; D003720:Densitometry; D038983:Galactolipids; D006017:Glycolipids / Q000302:isolation & purification; D008055:Lipids / Q000302:isolation & purification*; D009186:Myelin Sheath / Q000032:analysis*; D010743:Phospholipids / Q000302:isolation & purification; D051381:Rats; D013194:Staining and Labeling
D038983:Galactolipids; D006017:Glycolipids; D008055:Lipids; D010743:Phospholipids; D002784:Cholesterol