Localization of pp60c-src in growth cone of PC12 cell.
By immunocytochemical and biochemical techniques, we observed the localization and expression of pp60c-src in nerve growth factor (NGF)-treated PC12 cells. Immunostaining of pp60c-src is detected in the neuronal soma and the tips of neurites (growth cones). Immunofluorescence in the neurites is less significant. High-resolution microscopy reveals that the location of pp60c-src in growth cone is in good agreement with the adhesive site of growth cone to the substratum. The pp60c-src kinase activity and the pp60c-src protein level increase 3.1- to 3.5-fold and 2.0-fold during differentiation of PC12 cells, respectively. The pp60c-src levels in the neurite fraction are also higher than those in the neuronal soma fraction. These results support the immunocytochemical finding that pp60c-src is localized in growth cones of differentiated PC12 cells. Furthermore, we discuss the possible role of pp60c-src in growth cone.
Sobue
K
K
Department of Neurochemistry and Neuropharmacology, Osaka University Medical School, Japan.
Kanda
K
K
D000310:Adrenal Gland Neoplasms / Q000032:analysis* / Q000648:ultrastructure; D000818:Animals; D001369:Axons / Q000032:analysis*; D001839:Bombesin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002454:Cell Differentiation; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D007150:Immunohistochemistry; D008857:Microscopy, Interference; D009414:Nerve Growth Factors / Q000494:pharmacology; D009474:Neurons / Q000648:ultrastructure; D010673:Pheochromocytoma / Q000032:analysis* / Q000648:ultrastructure; D011494:Protein Kinases / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000032:analysis* / Q000378:metabolism; D016392:Proto-Oncogene Proteins pp60(c-src); D051381:Rats; D014407:Tumor Cells, Cultured
D009414:Nerve Growth Factors; D011518:Proto-Oncogene Proteins; C057644:bombesin, Leu(13)-psi-CH2NH-Leu(14)-; D011494:Protein Kinases; D016392:Proto-Oncogene Proteins pp60(c-src); D001839:Bombesin