Two screening methods show different antigen recognition patterns for four monoclonal antibodies to Candida albicans cell surface.
Four murine monoclonal antibodies (mAbs) showing similar reactivity with a cell-wall extract and mannan preparation obtained from Candida albicans were examined for epitope specificity. An enzyme-linked immunosorbent assay (ELISA) was used to determine total mAb binding to extracted cell-wall antigen when each mAb was reacted alone or in competition with a second mAb. This analysis suggested that three mAbs recognized the same determinant, which differed from that recognized by the fourth. The reactivity of these mAbs was also examined by indirect immunofluorescence assay with both yeast and germ tube forms of the dimorphic fungus. The three mAbs assigned the same epitope-specificity by ELISA showed two different patterns of reactivity with immunofluorescence. This discrepancy is discussed with respect to the postulated structure of mannan and the method of analysis.
Chaffin
W L
WL
Department of Microbiology, Texas Tech. University Health Sciences Center, Lubbock 79430.
Morrow
K J
KJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000954:Antigens, Surface / Q000032:analysis; D001666:Binding Sites, Antibody / Q000032:analysis; D015153:Blotting, Western; D002176:Candida albicans / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D008351:Mannans / Q000276:immunology; D051379:Mice; D008856:Microscopy, Fluorescence
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000939:Epitopes; D008351:Mannans