title: Leukotriene A4 hydrolase: analysis of some human tissues by radioimmunoassay.
abstract: Leukotriene A4 hydrolase was quantitated by radioimmunoassay, in extracts from eight human tissues. The enzyme was detectable in all tissues, with the highest level (2.6 mg per g soluble protein) in leukocytes, followed by lung and liver. The polyclonal antiserum did not cross-react with cytosolic epoxide hydrolase purified from mouse or human liver. When incubated with leukotriene A4, formation of leukotriene B4 was evident in all tissues. Furthermore, enzymatic formation of (5S,6R)-dihydroxy-7,9-trans-11,14-cis-eicosatetraenoic acid from leukotriene A4, was found in extracts from liver, kidney and intestines.
authors: Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden.
authors: J Y
authors: JY
authors: Fu
authors: J
authors: J
authors: Haeggström
authors: P
authors: P
authors: Collins
authors: J
authors: J
authors: Meijer
authors: O
authors: O
authors: Rådmark
mesh_terms: D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004851:Epoxide Hydrolases / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D011863:Radioimmunoassay; D013194:Staining and Labeling; D014018:Tissue Distribution
chemical_list: D004851:Epoxide Hydrolases; C043134:leukotriene A4 hydrolase