Canine and bovine ras family expression detected and discriminated by use of polymerase chain reaction.
Cellular proto-oncogenes are highly conserved genes thought to be critical in cell growth and differentiation. In this study, we used human sequence designed oligonucleotide primers to detect and discriminate c-Ha-ras-1, c-Ki-ras-2 and c-N-ras genes of dogs and cows by polymerase chain reaction (PCR) amplification of genomic DNA (DNA/PCR). Further, we have applied PCR for analysis of expressed mRNA transcribed from the RAS genes (RNA/PCR).
Madewell
B R
BR
Department of Veterinary Surgery, University of California School of Veterinary Medicine, Davis 95616.
Gumerlock
P H
PH
Saunders
K A
KA
Meyers
F J
FJ
Kraegel
S A
SA
Kawasaki
E S
ES
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004285:Dogs; D015870:Gene Expression; D011905:Genes, ras; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D014407:Tumor Cells, Cultured / Q000378:metabolism
D015345:Oligonucleotide Probes; D012313:RNA; D004247:DNA