Isolation of functionally active T cell receptor gamma delta-bearing lymphocytes from human peripheral blood.
We have developed a simple method for isolation of functionally active T cell receptor (TCR)-gamma delta positive cells from human peripheral blood, using immunomagnetic separation techniques. After culture with feeder cells and interleukin-2, cells thus isolated showed a CD3+ TCR-alpha beta- phenotype, and stained with antibodies against the TCR-gamma delta complex. The TCR-gamma delta+ cells were functionally active, responding with DNA synthesis when stimulated via their CD3 and CD2 molecules in concert, or when interleukin-2 was added.
Bosnes
V
V
Institute of Transplantation Immunology, National Hospital, Oslo, Norway.
Halvorsen
R
R
Gaudernack
G
G
Thorsby
E
E
D000328:Adult; D000911:Antibodies, Monoclonal; D002460:Cell Line; D002469:Cell Separation / Q000379:methods; D005434:Flow Cytometry; D006801:Humans; D007958:Leukocyte Count; D008280:Magnetics; D008863:Microspheres; D010641:Phenotype; D011948:Receptors, Antigen, T-Cell / Q000032:analysis; D013194:Staining and Labeling; D013601:T-Lymphocytes / Q000032:analysis / Q000145:classification* / Q000502:physiology
D000911:Antibodies, Monoclonal; D011948:Receptors, Antigen, T-Cell