Beta-glucuronidase and chloroacetate-esterase staining discriminates rat liver sinusoidal endothelial cells from Kupffer cells in primary culture.
Beta-glucuronidase and N-AS-D-chloroacetate esterase cytochemistry have been applied to rat liver sinusoidal endothelial cells and Kupffer cells. Both staining procedures allowed a clear-cut differentiation of either cell type. Kupffer cells which had been stained with beta-glucuronidase showed a positive reaction, whereas sinusoidal endothelial cells were completely negative. If the chloroacetate reaction was used, the former stained diffusely while the latter showed a characteristic granular staining pattern. Identity and purity of sinusoidal endothelial cells and Kupffer cells was validated by transmission and scanning electron microscopy as well as by the pattern of released eicosanoids which is characteristic for either cell type. These two staining techniques are a valuable addition to the peroxidase reaction commonly applied for differentiation.
Schlayer
H J
HJ
Biochemisches Institut, Albert-Ludwigs-Universität, Freiburg i Br., Federal Republic of Germany.
Woort-Menker
M
M
Eyhorn
S
S
Becker
H
H
Schaefer
H E
HE
Decker
K
K
D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000032:analysis*; D002478:Cells, Cultured; D004727:Endothelium / Q000166:cytology / Q000201:enzymology; D005966:Glucuronidase / Q000032:analysis*; D007728:Kupffer Cells / Q000201:enzymology / Q000648:ultrastructure*; D008099:Liver / Q000201:enzymology / Q000502:physiology / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling
D002265:Carboxylic Ester Hydrolases; C019873:chloroacetate esterase; D005966:Glucuronidase