Increase in RNA and protein synthesis by mitochondria irradiated with helium-neon laser.
To gain further insight into the mechanism of cell photostimulation by laser light, both RNA and protein synthesis were measured in mitochondria irradiated with the low power continuous wave He-Ne laser (Energy dose: 5 Joules/cm2). Following mitochondrial irradiation, both the rate and amount of incorporation of alpha-[32P]UTP and L-[35S]methionine, used to monitor RNA and protein synthesis respectively, proved to increase. Electrophoretic analysis made of the synthesis products clearly shows that He-Ne laser irradiation stimulates the synthesis of all mitochondrial transcription and translation products.
Greco
M
M
Centro di Studio sui Mitocondri e Metabolismo Energetico, C.N.R. Bari, Italy.
Guida
G
G
Perlino
E
E
Marra
E
E
Quagliariello
E
E
D000818:Animals; D006371:Helium; D007700:Kinetics; D007834:Lasers; D008715:Methionine / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism / Q000528:radiation effects*; D009356:Neon; D014176:Protein Biosynthesis; D011506:Proteins / Q000528:radiation effects*; D012313:RNA / Q000096:biosynthesis / Q000528:radiation effects*; D051381:Rats; D014544:Uridine Triphosphate / Q000378:metabolism
D011506:Proteins; D006371:Helium; D009356:Neon; D012313:RNA; D008715:Methionine; D014544:Uridine Triphosphate