Structural comparison of the 68 kDa laminin-binding protein and 5'-nucleotidase from chicken muscular sources: evidence against a gross structural similarity of both proteins.
The 68 kDa laminin-binding protein purified from chicken skeletal muscle and the ectoenzyme 5'-nucleotidase from chicken gizzard are both able to interact with laminin. They were both shown to possess a nearly identical amino acid composition. The 79 kDa glycosylated form of 5'-nucleotidase can be transformed into an enzymatically active form by treatment with endoglycosidase F (Endo F). Deglycosylated (Endo F-treated) 5'-nucleotidase exhibits an apparent molecular mass of 68 kDa. Using immunological and finger-printing techniques, both proteins were analysed to determine their structural relatedness. The results obtained indicate that both proteins are not identical but may posses a few common peptides of yet unknown sequence and length.
Risse
G
G
Max-Planck-Institut für Biochemie, Abteilung Bindegewebsforschung, Martinsried, F.R.G.
Stochaj
U
U
Elsässer
K
K
Dieckhoff
J
J
Mannherz
H G
HG
von der Mark
K
K
D015720:5'-Nucleotidase; D000244:Adenosine Diphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D000249:Adenosine Monophosphate / Q000378:metabolism; D000818:Animals; D000918:Antibody Specificity; D015153:Blotting, Western; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D000939:Epitopes / Q000276:immunology; D005895:Gizzard, Avian / Q000201:enzymology; D006026:Glycoside Hydrolases / Q000494:pharmacology; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D015394:Molecular Structure; D008970:Molecular Weight; D009132:Muscles / Q000032:analysis*; D009708:Nucleotidases / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism; D011971:Receptors, Immunologic / Q000276:immunology / Q000378:metabolism; D017474:Receptors, Laminin
D000939:Epitopes; D011971:Receptors, Immunologic; D017474:Receptors, Laminin; C523965:alpha,beta-methyleneadenosine 5'-diphosphate; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D009708:Nucleotidases; D015720:5'-Nucleotidase; D006026:Glycoside Hydrolases; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase