[The drug sensitivity of human gastric cancer implanted into the subcutis and stomach wall of nude mice].
This study was designed to establish a model able to predict the clinical efficacy of anticancer agents against cancers of specific organ. Seven weeks old, male BALB/c nude mice were implanted with 1 X 10(6) cells of human gastric cancer G/F into either their subcutis or the stomach wall. Fourteen days after the implantation, the mice were injected daily once for 10 days with peplomycin or mitomycin C. Peplomycin was effective on the subcutaneous tumors with an inhibition rate of 26 and 64% at doses of 1.5 and 6.0 mg/kg, respectively. Peplomycin was ineffective on the tumors in the stomach wall. Mitomycin C was ineffective on the subcutaneous tumors, but effective on the tumors in the stomach wall and the inhibition rate was 52 and 63% at doses of 0.13 and 0.5 mg/kg, respectively. Study on the distribution of peplomycin and mitomycin C to those tumors revealed that peplomycin and mitomycin C levels in the subcutaneous tumors were 2 to 7 times and about 3 times higher than those in the stomach wall, respectively. Thus, drug distribution could not explain the differences in drug sensitivity. The sensitivity of the tumor in the stomach wall to peplomycin and mitomycin C was consistent with the clinical efficacy of these drugs against human gastric cancers. This suggests that models where the tumors are implanted into its original organ are useful for predicting clinical efficacy in experimental cancer chemotherapy.
Yamashita
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D000230:Adenocarcinoma / Q000188:drug therapy* / Q000473:pathology; D000818:Animals; D001761:Bleomycin / Q000627:therapeutic use*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D016685:Mitomycin; D008937:Mitomycins / Q000627:therapeutic use*; D009368:Neoplasm Transplantation / Q000379:methods; D017663:Peplomycin; D012867:Skin / Q000473:pathology; D013270:Stomach / Q000473:pathology; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000473:pathology
D008937:Mitomycins; D001761:Bleomycin; D016685:Mitomycin; D017663:Peplomycin