In vivo and in vitro synthesis, release, and uptake of [3-H]-dopamine in mouse striatal slices after in vivo exposure to chlordecone.
Effects of treatment of mice with chlordecone (25 mg/kg/d) on striatal dopaminergic activities such as synthesis, turnover, uptake, and release were investigated in vivo and in vitro. In mice receiving chlordecone for five days, there were no significant changes in in vivo dopamine (DA) synthesis and turnover in striatum and in vitro [3-H]-dopamine uptake and K+-stimulated [3-H]-dopamine release in striatal slices. In mice receiving chlordecone for eight days, the in vivo synthesis of [3-H]-dopamine from [3-H]-tyrosine in striatum was slightly inhibited and the in vitro [3-H]-dopamine synthesis in striatal slices was significantly decreased. Furthermore, both uptake and K+-stimulated release of [3-H]-dopamine from striatal slices were significantly reduced. The turnover rate of newly synthesized [3-H]-dopamine from [3-H]-tyrosine in striatal slices was unchanged after eight consecutive days of chlordecone administration. These results suggest that chlordecone may cause impairments in pre- and/or postsynaptic membranes of dopaminergic neurons which modulate motor function.
Fujimori
K
K
Division of Pharmacology, National Institute of Hygienic Sciences, Tokyo, Japan.
Benet
H
H
Mehendale
H M
HM
Ho
I K
IK
D000818:Animals; D002395:Catecholamines / Q000096:biosynthesis; D007631:Chlordecone / Q000494:pharmacology*; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D004298:Dopamine / Q000096:biosynthesis / Q000378:metabolism* / Q000493:pharmacokinetics; D007306:Insecticides / Q000494:pharmacology*; D008297:Male; D008781:Methyltyrosines / Q000494:pharmacology; D051379:Mice; D014443:Tyrosine / Q000378:metabolism; D019805:alpha-Methyltyrosine
D002395:Catecholamines; D007306:Insecticides; D008781:Methyltyrosines; D014443:Tyrosine; D019805:alpha-Methyltyrosine; D007631:Chlordecone; D004298:Dopamine