Linkage relationships of the protein kinase C gamma gene which exclude it as a candidate for myotonic dystrophy.
Using a cDNA probe for the gamma gene of protein kinase C (PKCG), an informative RFLP with a PIC value of 0.62 has been identified with the enzyme MspI. The polymorphic bands have been assigned to chromosome 19. Analysis of the segregation of alleles for this probe in myotonic dystrophy families show several recombinants between PKCG and myotonic dystrophy (DM) and exclude this gene as a candidate for DM. Linkage relationships between PKCG and other loci on chromosome 19 are presented which exclude PKCG from the proximal region of chromosome 19 and which are consistent with the localization being at 19q13.2----qter.
Johnson
K J
KJ
Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, University of London, UK.
Jones
P J
PJ
Spurr
N
N
Nimmo
E
E
Davies
J
J
Creed
H
H
Weiss
M
M
Williamson
R
R
D000483:Alleles; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D006822:Hybrid Cells; D009223:Myotonic Dystrophy / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010375:Pedigree; D012150:Polymorphism, Restriction Fragment Length; D011493:Protein Kinase C / Q000235:genetics*
D015342:DNA Probes; D005819:Genetic Markers; D004247:DNA; D011493:Protein Kinase C; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific