Insertion of short hepatitis virus A amino acid sequences into poliovirus antigenic determinants results in viable progeny.
In an infectious poliovirus cDNA construct, the determinant encoding antigenic epitope N-Ag1 (in a loop located between two beta-strands in poly-peptide VP1) was altered by site-directed mutagenesis, to be partially similar with the determinants for presumptive epitopes in polypeptides VP1 or VP3 of hepatitis A virus (HAV). The modified constructs proved to be infectious. However, another construct, in which the same locus encoded a 'nonsense' and a relatively hydrophobic amino acid sequence, exhibited no infectivity. These data showed the feasibility of the insertion of foreign sequences in a specific antigenically active locus of the poliovirus icosahedron, and suggest some limitations with respect to the sequences to be 'transplanted'.
Sverdlov
E D
ED
M.M. Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow.
Tsarev
S A
SA
Markova
S V
SV
Rostapshov
V M
VM
Azhikina
T L
TL
Chernov
I P
IP
Gorbalenya
A E
AE
Kolesnikova
M S
MS
Romanova
L I
LI
Teterina
N L
NL
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics; D000939:Epitopes; D000882:Haplorhini; D006507:Hepatovirus / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D010957:Plasmids; D017955:Poliovirus / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D011055:Poliovirus Vaccine, Oral / Q000235:genetics; D015183:Restriction Mapping; D014612:Vaccines / Q000235:genetics*; D014614:Vaccines, Synthetic / Q000235:genetics*; D014765:Viral Vaccines / Q000276:immunology*
D000939:Epitopes; D011055:Poliovirus Vaccine, Oral; D014612:Vaccines; D014614:Vaccines, Synthetic; D014765:Viral Vaccines; D004247:DNA