title: Divergent effects of acute depolarization on somatostatin release and protein synthesis in cultured fetal and neonatal rat brain cells.
abstract: The influence of membrane depolarization on somatostatin secretion and protein synthesis by fetal and neonatal cerebrocortical neurons was studied. Cortical cells obtained by mechanical dispersion were maintained as monolayer cultures for 8 days. The ability of fetal cerebrocortical and hypothalamic cells to release immunoreactive somatostatin (IR-SRIF) was confirmed. Total protein synthesis was determined by the incorporation of [3H]phenylalanine into trichloroacetic acid-precipitable proteins. To study the effect of acute depolarization on protein synthesis, cells were incubated for 30 min with [3H]phenylalanine or [3H]leucine and the depolarizing agent. In fetal cerebrocortical cells, potassium (30 and 56 mM) decreased protein synthesis and RNA levels and increased IR-SRIF release. Depolarization by veratridine, a sodium channel activator, induced a similar effect. The effect of veratridine on IR-SRIF and protein synthesis was reversed by tetrodotoxin, a sodium channel blocker, or verapamil, a calcium channel blocker. These findings suggest that protein synthesis by cerebrocortical cells is decreased in fetal brain cells by membrane depolarization and is dependent on Na+ and Ca2+ entry into cells. In postnatal (day 7) cerebrocortical cells, depolarization induced by high potassium concentrations led to a concomitant increase in protein synthesis, RNA content, and somatostatin release. These findings indicate that depolarization of the cellular membrane is coupled to an increase in protein synthesis in neonatal, but not in fetal, dispersed brain cells.
authors: Servicio de Endocrinologia, Hospital Ramón y Cajal, Madrid, Spain.
authors: M T
authors: MT
authors: de los Frailes
authors: L
authors: L
authors: Cacicedo
authors: M J
authors: MJ
authors: Lorenzo
authors: F
authors: F
authors: Sánchez-Franco
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000502:physiology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000187:drug effects / Q000196:embryology / Q000502:physiology*; D005333:Fetus / Q000502:physiology*; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008564:Membrane Potentials; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D010649:Phenylalanine / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000378:metabolism; D013004:Somatostatin / Q000378:metabolism*; D013779:Tetrodotoxin / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology; D014701:Veratridine / Q000494:pharmacology
chemical_list: D009419:Nerve Tissue Proteins; D013779:Tetrodotoxin; D010649:Phenylalanine; D013004:Somatostatin; D012313:RNA; D014701:Veratridine; D012964:Sodium; D014700:Verapamil; D007930:Leucine; D011188:Potassium; D002118:Calcium