The use of an electroporation apparatus for the production of murine hybridomas.
Antigen-directed electrofusion was carried out using biotin-streptavidin to bridge antigen-specific splenocytes to myeloma cells. Electrofusion was performed using a commercial electroporation apparatus. Electrofusion conditions were optimized by measuring the survival of myeloma cells after a range of electrical pulse conditions. The procedure was tested with antigens of high and low immunogenicity. The yields of hybridomas secreting antibodies specific for both antigens were considerably increased by the use of the electrofusion procedure.
Hewish
D R
DR
CSIRO, Division of Biotechnology, Parkville, Victoria, Australia.
Werkmeister
J A
JA
D000818:Animals; D001426:Bacterial Proteins; D001710:Biotin; D002459:Cell Fusion / Q000379:methods*; D003094:Collagen / Q000276:immunology; D004560:Electricity; D006825:Hybridomas; D066298:In Vitro Techniques; D007633:Keratins / Q000276:immunology; D051379:Mice; D019809:Streptavidin
D001426:Bacterial Proteins; D007633:Keratins; D001710:Biotin; D003094:Collagen; D019809:Streptavidin