Inhibition of Moloney murine leukemia virus reverse transcriptase by alpha-anomeric oligonucleotides.
After parallel hybridization to complementary template RNA, alpha-anomeric oligonucleotides are not primers for Moloney murine leukemia virus reverse transcriptase. As can be expected they are competitors of classical primer oligonucleotides (beta-anomeric). They therefore inhibit the RNA dependent DNA polymerase activity of Moloney murine leukemia virus reverse transcriptase with either homopolymeric or heteropolymeric substrates. Non complementary alpha-oligonucleotides display no inhibitory activity. alpha-Oligonucleotides are therefore potential candidates for inhibition of retroviral reverse transcriptases by interference with the primer binding sites.
Lavignon
M
M
UA 147 CNRS, U 140 INSERM, Institut Gustave Roussy, Villejuif, France.
Bertrand
J R
JR
Rayner
B
B
Imbach
J L
JL
Malvy
C
C
Paoletti
C
C
D001483:Base Sequence; D007536:Isomerism; D007700:Kinetics; D008979:Moloney murine leukemia virus / Q000201:enzymology*; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D018894:Reverse Transcriptase Inhibitors; D013329:Structure-Activity Relationship; D013698:Templates, Genetic
D009838:Oligodeoxyribonucleotides; D018894:Reverse Transcriptase Inhibitors