Selective imipenem resistance in Pseudomonas aeruginosa associated with diminished outer membrane permeability.
The permeability of the outer membranes of imipenem-susceptible and imipenem-resistant clinical isolates of Pseudomonas aeruginosa was investigated by the liposome swelling assay. Sugars and cephaloridine penetrated rapidly, whereas imipenem penetrated poorly into liposomes constructed from porin-rich outer membrane fractions of the resistant isolates.
Studemeister
A E
AE
Department of Medicine, Loyola University Medical Center, Maywood, Illinois 60153.
Quinn
J P
JP
D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D002463:Cell Membrane Permeability; D002499:Centrifugation, Density Gradient; D002509:Cephaloridine / Q000378:metabolism; D004352:Drug Resistance, Microbial; D005947:Glucose / Q000378:metabolism; D006801:Humans; D015378:Imipenem / Q000378:metabolism / Q000494:pharmacology*; D007473:Ion Channels / Q000378:metabolism; D008081:Liposomes / Q000378:metabolism; D018272:Porins; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000378:metabolism
D001425:Bacterial Outer Membrane Proteins; D007473:Ion Channels; D008081:Liposomes; D018272:Porins; D015378:Imipenem; D005947:Glucose; D002509:Cephaloridine