Infection of rabbits with human immunodeficiency virus 1. A small animal model for acquired immunodeficiency syndrome.
Injection of rabbits with a human T cell line infected with HIV-1 caused seroconversion within 6 wk, and HIV-1 could be isolated from PBL cultures of infected rabbits. Identity of the isolated virus with HIV-1 was shown by analysis of products amplified by the polymerase chain reaction. HIV-1 infection was seen in rabbits injected with HIV-1-infected cells alone as well as in those that were first infected with HTLV-1 and subsequently with HIV-1. There were no consistent signs of disease in the rabbits infected with HIV-1 alone but HTLV-1/HIV-1-infected rabbits showed signs of illness including diarrhea and weight loss, transient neurologic impairment and, in one animal, a rapidly progressing mammary adenocarcinoma. Examination of organs taken at necropsy from both HIV-1- and HTLV-1/HIV-1-infected animals showed splenic hyperplasia and lymphocyte infiltration of the lungs, as well as moderate damage to liver and kidney in some cases.
Kulaga
H
H
Laboratory of Immunogenetics, National Institute of Allergy and Infectious Disease, Bethesda, Maryland 20892.
Folks
T
T
Rutledge
R
R
Truckenmiller
M E
ME
Gugel
E
E
Kindt
T J
TJ
D000163:Acquired Immunodeficiency Syndrome / Q000503:physiopathology*; D000818:Animals; D004279:DNA, Viral / Q000032:analysis; D004195:Disease Models, Animal; D006679:HIV Seropositivity / Q000503:physiopathology; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D011817:Rabbits; D013997:Time Factors
D004279:DNA, Viral; D012194:RNA-Directed DNA Polymerase