Noninterference of isosulfan blue on estrogen-receptor activity.
Because a 1% sterile solution of methylene blue used for occult breast tumor localization has been shown to interfere with the estrogen-receptor protein (ERP) binding-capacity assay, isosulfan blue in a 1% injection was studied as a potential alternate stain. Cytosols derived from ERP-positive lyophilized powders and human breast tissue were evaluated with and without varying levels of treatment with isosulfan blue. No modification of the ERP-specific binding capacity was found with this stain. The use of isosulfan blue for localization of occult breast tumor is suggested when an ERP binding capacity assay is anticipated.
Hirsch
J I
JI
Department of Radiology, Massey Cancer Center, Richmond, VA.
Banks
W L
WL
Sullivan
J S
JS
Horsley
J S
JS
D001707:Biopsy, Needle; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000473:pathology; D003600:Cytosol / Q000187:drug effects; D005260:Female; D006801:Humans; D008327:Mammography; D011960:Receptors, Estrogen / Q000187:drug effects*; D012394:Rosaniline Dyes / Q000494:pharmacology; D013194:Staining and Labeling
D011960:Receptors, Estrogen; D012394:Rosaniline Dyes; C025484:iso-sulfan blue