Isolation and characterization of a cDNA for mitochondrial manganese superoxide dismutase (SOD-3) of maize and its relation to other manganese superoxide dismutases.
We describe the isolation of a cDNA clone for the nuclear-encoded manganese superoxide dismutase (SOD-3) of maize mitochondria. The cDNA, pSod3.1c, selects by hybridization an RNA which produces the SOD-3 precursor upon in vitro translation. The DNA sequence of pSod3.1c was determined from fragments subcloned in M13. The amino-acid sequence deduced from the nucleotide sequence displays significant homology with the amino-acid sequences of prokaryotic and eukaryotic Mn-SODs, but displays greater homology with mammalian Mn-SOD than it does with yeast or bacterial Mn-SOD. A 31 amino-acid transit peptide also is encoded by the pSod3.1c clone. Analysis of poly(A)+ RNA indicates that Sod3 mRNA is approx. 1250 nucleotides in length. The amount of Sod3 transcript in seedling leaves is increased by light.
White
J A
JA
Department of Genetics, North Carolina State University Raleigh 27695-7614.
Scandalios
J G
JG
D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D007163:Immunosorbent Techniques; D007527:Isoenzymes / Q000235:genetics*; D008345:Manganese; D008928:Mitochondria / Q000201:enzymology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010944:Plants / Q000201:enzymology* / Q000235:genetics; D011061:Poly A / Q000235:genetics; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D012689:Sequence Homology, Nucleic Acid; D013482:Superoxide Dismutase / Q000235:genetics*; D003313:Zea mays
D007527:Isoenzymes; D012333:RNA, Messenger; D011061:Poly A; D008345:Manganese; D012313:RNA; D004247:DNA; D013482:Superoxide Dismutase