title: Pores from mitochondrial outer membranes of yeast and a porin-deficient yeast mutant: a comparison.
abstract: Reconstitution experiments were performed on lipid bilayer membranes in the presence of purified mitochondrial porin from yeast and of detergent-solubilized mitochondrial outer membranes of a porin-free yeast mutant. The addition of the porin resulted in a strong increase of the membrane conductance, which was caused by the formation of ion-permeable channels in the membranes. Yeast porin has a single-channel conductance of 4.2 nS in 1 M KCl. In the open state it behaves as a general diffusion pore with an effective diameter of 1.7 nm and possesses properties similar to other mitochondrial porins. Surprisingly, the membrane conductance also increased in the presence of detergent extracts of the mitochondrial outer membrane of the mutant. Single-channel recordings of lipid bilayer membranes in the presence of small concentration of the mutant membranes suggested that this membrane also contained a pore. The reconstituted pores had a single-channel conductance of 2.0 nS in 1 M KCl and the characteristics of general diffusion pores with an estimated effective diameter of 1.2 nm. This means that the pores present in the mitochondrial outer membranes of the yeast mutant have a much smaller effective diameter than "normal" mitochondrial porins. Zero-current membrane potential measurements suggested that the second mitochondrial porin is slightly cation-selective, while yeast porin is slightly anion-selective in the open state but highly cation-selective in the closed state. The possible role of these pores in the metabolism of mitochondria is discussed.
authors: Lehrstuhl für Biotechnologie, Universität Würzburg, F.R.G.
authors: R
authors: R
authors: Benz
authors: A
authors: A
authors: Schmid
authors: M
authors: M
authors: Dihanich
mesh_terms: D004553:Electric Conductivity; D007473:Ion Channels / Q000378:metabolism; D008051:Lipid Bilayers; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D018272:Porins; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D050994:Voltage-Dependent Anion Channels
chemical_list: D007473:Ion Channels; D008051:Lipid Bilayers; D008565:Membrane Proteins; D018272:Porins; D050994:Voltage-Dependent Anion Channels