Protein tyrosine phosphorylation in response to fertilization.
The sea urchin egg contains one or more protein tyrosine kinases which are active during the response of the egg to fertilization. In the present study, we have used an antibody specific for phosphotyrosine to determine which egg proteins are phosphorylated on tyrosine in response to fertilization. Analysis of immunoblots prepared from fertilized and unfertilized eggs revealed that fertilization results in a major increase in the phosphotyrosine content of a 350-kDa egg protein. Increased phosphorylation of this protein was detected as early as 1 min after fertilization, at which time it represented the most prominent phosphotyrosine containing protein in the egg. Tyrosine phosphorylation of this protein was transient however, and after 5 min post-insemination, the protein was dephosphorylated or otherwise degraded. Egg membrane proteins of approximately 40, 75, and 145 kDa were also found to act as substrates for protein tyrosine kinases in vitro, but did not exhibit significant changes in phosphotyrosine content during egg activation.
Peaucellier
G
G
Department of Anatomy and Cell Biology, University of Miami School of Medicine, Florida 33101.
Veno
P A
PA
Kinsey
W H
WH
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002499:Centrifugation, Density Gradient; D004527:Egg Proteins / Q000032:analysis / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005306:Fertilization; D007118:Immunoassay; D007163:Immunosorbent Techniques; D008970:Molecular Weight; D010063:Ovum / Q000032:analysis*; D010769:Phosphothreonine / Q000032:analysis; D019000:Phosphotyrosine; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D012617:Sea Urchins / Q000196:embryology*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism
D004527:Egg Proteins; D010769:Phosphothreonine; D019000:Phosphotyrosine; D014443:Tyrosine; D000255:Adenosine Triphosphate; D011505:Protein-Tyrosine Kinases