Gene transfer of a putative mammalian K+ channel gene from genomic and cosmid DNA.
We have successfully transfected mouse genomic DNA containing a mutant putative K+ channel gene into murine LMTK- cells. Incorporation of the mutant gene allowed primary transformants to express the mutant cell phenotype of growth at a subthreshold K+ concentration and, furthermore, caused these primary transformants to display the same characteristics of growth and unidirectional K+ flux as the mutant cell line, LTK-1, from which the genomic DNA was derived. A cosmid genomic library was formed from LTK-1 mutant DNA and shown by transfection experiments to contain at least one unique cosmid clone containing a functional copy of the mutant putative K+ channel gene.
Mitas
M
M
Department of Physiology, Emory University School of Medicine, Atlanta, Georgia 30322.
Coogan
C
C
Gargus
J J
JJ
D000212:Acyclovir / Q000494:pharmacology; D000818:Animals; D003360:Cosmids; D004247:DNA / Q000032:analysis*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015245:Deoxyribonuclease BamHI; D007473:Ion Channels / Q000378:metabolism*; D051379:Mice; D011188:Potassium / Q000378:metabolism*; D014162:Transfection
D007473:Ion Channels; D004247:DNA; D004262:DNA Restriction Enzymes; D015245:Deoxyribonuclease BamHI; D011188:Potassium; D000212:Acyclovir