Rapid detection of HIV-1 in clinical samples by co-culture with heat-shocked cells.
A mild heat-shock (10 min at 44 degrees C), performed just before seeding on healthy PBL used for co-cultivation, allows the detection of viral markers (p24 core antigen and reverse transcriptase) in a very short time. This suggests that heat-shocked PBL can be employed routinely for the rapid detection of HIV-1 in clinical samples.
Re
M C
MC
Institute of Microbiology, University of Bologna, Italy.
Furlini
G
G
La Placa
M
M
D002478:Cells, Cultured; D015683:Gene Products, gag / Q000032:analysis*; D015488:HIV Antigens / Q000032:analysis*; D016655:HIV Core Protein p24; D015497:HIV-1 / Q000276:immunology / Q000302:isolation & purification* / Q000502:physiology; D006358:Hot Temperature; D006801:Humans; D008214:Lymphocytes / Q000382:microbiology*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D014758:Viral Core Proteins / Q000032:analysis*; D014779:Virus Replication
D015683:Gene Products, gag; D015488:HIV Antigens; D016655:HIV Core Protein p24; D014758:Viral Core Proteins; D012194:RNA-Directed DNA Polymerase