Cyclic AMP mediation of the hormonal stimulation of 14C-acetate incorporation by Heliothis armigera pheromone glands in vitro.
Evidence is presented for hormone-controlled adenosine 3',5'-cyclic monophosphate (cAMP)-mediated incorporation of 14C-acetate by in vitro pheromone gland preparations of Heliothis armigera. Basal intracellular cAMP concentrations of in vitro pheromone glands increase 28-fold in the presence of methanolic brain-complex extracts after 5 min of incubation. This intracellular cAMP increase shows a dose-dependent response. Studies on isolated pheromone gland preparations show that 8-bromo-cAMP added to the incubation media stimulates acetate incorporation thereby mimicking the hormonal response. This effect was duplicated by forskolin and isobutyl-methylxanthine. In addition, forskolin exhibits a dose-dependent stimulatory effect on the incorporation of 14C-acetate by such isolated in vitro pheromone glands. High-pressure liquid chromatographic analysis of the radioactive products, extracted by hexane from pheromone glands and media, revealed a stimulation by forskolin of a product exhibiting the same retention time as (Z)-11-hexadecenal, the main pheromone component of H. armigera. In addition, thin-layer chromatographic analysis revealed co-elution with (Z)-11-hexadecenal on stimulation by 8-bromo-cAMP.
Rafaeli
A
A
Department of Entomology, Faculty of Agriculture, Rehovot, Israel.
Soroker
V
V
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000085:Acetates / Q000378:metabolism; D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000502:physiology; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000502:physiology*; D005260:Female; D066298:In Vitro Techniques; D007915:Lepidoptera / Q000378:metabolism*; D010675:Pheromones / Q000378:metabolism*; D012724:Sex Attractants / Q000378:metabolism*; D014020:Tissue Extracts / Q000494:pharmacology
D000085:Acetates; D000447:Aldehydes; D010675:Pheromones; D012724:Sex Attractants; D014020:Tissue Extracts; D005576:Colforsin; D015124:8-Bromo Cyclic Adenosine Monophosphate; C061128:11-hexadecenal; D000242:Cyclic AMP; D015056:1-Methyl-3-isobutylxanthine