title: Production and ELISA application of bispecific monoclonal antibodies against fluorescein isothiocyanate (FITC) and horseradish peroxidase (HRP).
abstract: Hybrid hybridomas producing bispecific monoclonal antibodies reacting with both horseradish peroxidase (HRP) and fluorescein isothiocyanate (FITC) were obtained by fusing two hybridoma lines and selecting the fused cells using a fluorescence activated cell sorter (FACS). FITC was used to label different monoclonal antibodies and the bispecific antibodies acted as a linking agent between FITC-labelled antibody and the marker enzyme HRP. This system was used in enzyme immunoassays for the detection of different antigens. The results suggest a wide application of bispecific anti-FITC/anti-HRP antibodies as a detection system in EIA.
authors: Central Institute of Molecular Biology, Academy of Sciences of the GDR, Berlin.
authors: L
authors: L
authors: Karawajew
authors: O
authors: O
authors: Behrsing
authors: G
authors: G
authors: Kaiser
authors: B
authors: B
authors: Micheel
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D002459:Cell Fusion; D006063:Chorionic Gonadotropin / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins / Q000276:immunology*; D006735:Horseradish Peroxidase / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D007202:Indicators and Reagents; D051379:Mice; D008807:Mice, Inbred BALB C; D010544:Peroxidases / Q000276:immunology*; D013861:Thiocyanates / Q000276:immunology*; D000509:alpha-Fetoproteins / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D006063:Chorionic Gonadotropin; D005452:Fluoresceins; D007202:Indicators and Reagents; D013861:Thiocyanates; D000509:alpha-Fetoproteins; D006735:Horseradish Peroxidase; D010544:Peroxidases; D016650:Fluorescein-5-isothiocyanate