Single-channel recordings from the apical membrane of the toad urinary bladder epithelial cell.
The patch-clamp technique for the recording of single-channel currents was used to investigate the activity of ion channels in the intact epithelium of the toad urinary bladder. High resistance seals were obtained from the apical membrane of tightly stretched tissue. Single-channel recordings revealed the activity of a variety of ion channels that could be classified in 4 groups according to their mean ion conductances, ranging from 5 to 59 pS. In particular, we observed highly selective, amiloride-sensitive Na channels with a mean conductance of 4.8 pS, channels with a similar conductance that were not Na-selective and channels with mean conductance values of 17-58 pS that were mostly seen after stimulation of the tissue with vasopressin or cAMP. When inside-out patches from the apical membrane were exposed to 110 mM fluoride, large conductances (86-490 pS) appeared.
Frings
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S
Department of Physiology, University of Otago Medical School, Dunedin, New Zealand.
Purves
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RD
Macknight
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AD
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001949:Bretylium Compounds; D002024:Bufo marinus; D000242:Cyclic AMP / Q000494:pharmacology; D004553:Electric Conductivity; D001330:Electronic Data Processing; D004848:Epithelium / Q000378:metabolism; D005260:Female; D005459:Fluorides / Q000378:metabolism; D066298:In Vitro Techniques; D007473:Ion Channels / Q000378:metabolism*; D008566:Membranes / Q000378:metabolism; D008722:Methods; D008954:Models, Biological; D011494:Protein Kinases / Q000494:pharmacology; D015222:Sodium Channels / Q000378:metabolism; D001743:Urinary Bladder / Q000378:metabolism*; D014667:Vasopressins / Q000494:pharmacology
D001949:Bretylium Compounds; D007473:Ion Channels; D015222:Sodium Channels; D014667:Vasopressins; D000584:Amiloride; D000242:Cyclic AMP; D011494:Protein Kinases; D005459:Fluorides; C045166:bretylium