Role of the LFA3-CD2 interaction in human specific B cell differentiation.
We examined the role of the lymphocyte function-associated (LFA)3 molecule in human B cell response. A mAb to this molecule did not influence B cell proliferation induced by anti-mu antibody and IL. In contrast, the same mAb inhibited the specific T-dependent B cell response induced by a particulate Ag. In the same line, two anti-CD2 mAb (directed toward the T11-1 and T11-2 epitopes) inhibited this response, whether used alone or in association. These inhibitions took place at an early stage of the response, and anti-LFA3 and anti-CD2 mAb acted on B cells and T cells, respectively. In contrast, when T cell help was provided by exogenous IL-2, the B cell response was resistant to the inhibitory effect of anti-LFA3 mAb. Taken together, these results indicate that the LFA3-CD2 pair play a major role in the direct T-B interaction required for T cell help.
Emilie
D
D
INSERM Unité, Clamart, France.
Wallon
C
C
Galanaud
P
P
Fischer
A
A
Olive
D
D
Delfraissy
J F
JF
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology / Q000502:physiology*; D000954:Antigens, Surface / Q000276:immunology / Q000502:physiology*; D001402:B-Lymphocytes / Q000276:immunology / Q000502:physiology*; D001667:Binding, Competitive; D018801:CD2 Antigens; D018968:CD58 Antigens; D002454:Cell Differentiation; D006801:Humans; D007166:Immunosuppressive Agents / Q000502:physiology; D007376:Interleukin-2 / Q000494:pharmacology; D015847:Interleukin-4; D007378:Interleukins / Q000502:physiology; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D008562:Membrane Glycoproteins / Q000276:immunology / Q000502:physiology*; D051379:Mice; D009000:Monocytes / Q000276:immunology; D011971:Receptors, Immunologic / Q000276:immunology / Q000502:physiology*; D014301:Trinitrobenzenes / Q000276:immunology
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D018801:CD2 Antigens; D018968:CD58 Antigens; D007166:Immunosuppressive Agents; D007376:Interleukin-2; D007378:Interleukins; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; D014301:Trinitrobenzenes; D015847:Interleukin-4