Identification by flow cytometry of two distinct rhodamine-123-stained mitochondrial populations in rat liver.
Isolated rat liver mitochondria were split into three fractions of increasing density when applied to a Percoll gradient. NADH-ubiquinone oxidoreductase, succinate dehydrogenase and cytochrome-c oxidase but not F1-ATPase activities increased with density as well as respiratory rate in state 3 and the respiratory control index. Flow cytometry of mitochondrial density fractions stained with rhodamine-123 revealed the occurrence in each density fraction of two distinct mitochondrial populations with different fluorescence intensity. The high fluorescence population was minor and its proportion decreased with density. The extent of high fluorescence population staining depended on the deenergized state of the mitochondria suggesting that this population represents an immature form of the mitochondria which may develop into a fully functional organelle by the incorporation of structural and/or functional proteins.
Lopez-Mediavilla
C
C
Departmento de Bioquimica y Biologia Molecular, Universidad de Salamanca, Spain.
Orfao
A
A
Gonzalez
M
M
Medina
J M
JM
D000818:Animals; D002458:Cell Fractionation; D003576:Electron Transport Complex IV / Q000032:analysis; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D008930:Mitochondria, Liver / Q000166:cytology / Q000201:enzymology*; D016660:NAD(P)H Dehydrogenase (Quinone); D010101:Oxygen Consumption; D011808:Quinone Reductases / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D020112:Rhodamine 123; D012235:Rhodamines; D013194:Staining and Labeling; D013329:Structure-Activity Relationship; D013385:Succinate Dehydrogenase / Q000032:analysis; D014966:Xanthenes
D005456:Fluorescent Dyes; D012235:Rhodamines; D014966:Xanthenes; D020112:Rhodamine 123; D013385:Succinate Dehydrogenase; D016660:NAD(P)H Dehydrogenase (Quinone); D011808:Quinone Reductases; D003576:Electron Transport Complex IV