A comparison of competitive enzyme immunoassay and precipitin inhibition tests in the analysis of polysaccharide antigenic determinants of oral streptococci.
A rapid and convenient method of competitive enzyme immunoassay (EIA) was explored for the immunological analysis by MAbs of polysaccharide antigens prepared from the mutans group of streptococci, and optimal conditions for it were established. These included the concentrations of antigen, monoclonal antibodies (MAbs) and labelled antibody, temperature, pH and reaction time. The results were compared with those obtained by precipitin inhibition tests using the classical quantitative precipitin reaction, and they were found to be consistent with the latter method. It was concluded that the EIA system can be extended to the immunological study of polysaccharide antigens of oral streptococci using MAbs which perform poorly in precipitation reactions.
Ota
F
F
Department of Oral Microbiology and Immunology, School of Dentistry, University of Tokushima, Japan.
Hirota
K
K
Nagamune
H
H
Senesi
S
S
Satomi
Y
Y
Yasuoka
M
M
Akiyama
Y
Y
Fukui
K
K
Kawata
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D000939:Epitopes / Q000276:immunology*; D005069:Evaluation Studies as Topic; D006863:Hydrogen-Ion Concentration; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D009055:Mouth / Q000382:microbiology; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D011233:Precipitin Tests; D013291:Streptococcus / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes; D011135:Polysaccharides, Bacterial