The impact of boundary conditions in free flow field step electrophoresis.
The impact of the type of membrane used to separate the electrolyte compartments from the separation cell for continuous flow field step electrophoresis is examined using both experimental and computer simulation data. It is shown that the distinct transport characteristics of ion exchange membranes and dialysis membranes may have an effect on the stability of the pH and conductivity gradients created by the buffer system. These effects are interpreted using computer simulation data. The limitations of the model used to portray the transport characteristics of ion exchange membranes are discussed as are the experimental conditions which provide the greatest stability for field step electrophoresis.
Heydt
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A
Center for Separation Science, University of Arizona, Tucson 85721.
Mosher
R A
RA
D002021:Buffers; D002462:Cell Membrane / Q000502:physiology; D003198:Computer Simulation; D003956:Dialysis; D004563:Electrochemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods*; D007473:Ion Channels; D037102:Lectins; D037121:Plant Lectins
D002021:Buffers; D007473:Ion Channels; D037102:Lectins; D037121:Plant Lectins; C033619:lentil lectin