Inhibitory action of galanin on gastric acid secretion in pentobarbital-anesthetized rats.
The effects of galanin and two galanin fragments, GAL(9-29) and GAL(15-29), were studied for potential effects on pentagastrin- and bethanechol-stimulated gastric acid secretion in a pentobarbital-anesthetized rat experimental model. At a dose of 10 micrograms/kg/h, galanin potently inhibited pentagastrin-stimulated gastric acid secretion whereas inhibition of bethanechol-stimulated gastric acid secretion was not statistically significant. Simultaneous iv infusion of galanin and atropine did not affect the inhibitory action of the former. In similar experiments, a GAL(15-29) fragment was completely inactive whilst GAL(9-29) retained only about 5% potency. These results indicate that galanin probably induces its inhibitory effects by acting directly on the parietal cells rather than through a cholinergic pathway. They also demonstrate that the rat gastric acid inhibitory activity of galanin depends critically on the integrity of the first fourteen N-terminal amino acids.
Rossowski
W J
WJ
Department of Medicine, Tulane University School of Medicine, New Orleans, Louisiana 70112.
Coy
D H
DH
D000818:Animals; D018723:Bethanechol; D001626:Bethanechol Compounds / Q000494:pharmacology; D019004:Galanin; D005750:Gastric Juice / Q000378:metabolism*; D008297:Male; D010418:Pentagastrin / Q000494:pharmacology; D010424:Pentobarbital / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012636:Secretory Rate / Q000187:drug effects; D013329:Structure-Activity Relationship
D001626:Bethanechol Compounds; D010446:Peptide Fragments; D010455:Peptides; D018723:Bethanechol; D019004:Galanin; D010418:Pentagastrin; D010424:Pentobarbital