A third T-cell receptor beta-chain variable region gene encodes reactivity to Mls-1a gene products.
Using a quantitative RNA hybridization method, we have identified a third murine T-cell receptor beta-chain variable (V) region gene, V beta 9, that engenders recognition of Mls-1a gene products. V beta 9-expressing T cells are clonally deleted from the periphery of mice that carry the Mls-1a allele, and the fine specificity of V beta 9+ hybridomas suggests that V beta 6-, V beta 8.1-, and V beta 9-encoded T-cell receptors may recognize a similar antigenic epitope. Comparison of these three protein sequences identifies two residues that may be important in determining this recognition specificity.
Happ
M P
MP
Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, Denver, CO 80206.
Woodland
D L
DL
Palmer
E
E
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D002872:Chromosome Deletion; D005434:Flow Cytometry; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D006825:Hybridomas / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology; D014158:Transcription, Genetic
D000911:Antibodies, Monoclonal; D011948:Receptors, Antigen, T-Cell; D012313:RNA