title: 3'-end labeling of DNA fragments by AMV-reverse transcriptase.
abstract: The efficiency on 3'-end labeling of DNA fragments was compared between Klenow fragment of DNA polymerase I from E. coli and AMV-reverse transcriptase. In the case of Klenow fragment, the exonuclease activity of this enzyme rapidly removed the labeled dNMP incorporated in 3'-end of DNA fragment by the polymerase activity of it. AMV-reverse transcriptase caused no decrease of the labeled dNMP incorporated by the polymerase activity because it has no exonuclease activity. Therefore, AMV-reverse transcriptase is easier to use than Klenow fragment for labeling the 3'-end of DNA fragment.
authors: Mitsubishi-Kasei Institute of Life Sciences, Tokyo, Japan.
authors: F
authors: F
authors: Oyama
authors: R
authors: R
authors: Kikuchi
authors: A
authors: A
authors: Omori
authors: T
authors: T
authors: Uchida
mesh_terms: D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D004247:DNA; D004256:DNA Polymerase I; D004926:Escherichia coli / Q000201:enzymology; D007553:Isotope Labeling / Q000379:methods; D012194:RNA-Directed DNA Polymerase
chemical_list: D004247:DNA; D012194:RNA-Directed DNA Polymerase; D004256:DNA Polymerase I