The stable prostacyclin-analogue, iloprost, unlike prostanoids and leukotrienes, potently stimulates cyclic adenosine monophosphate synthesis of primary astroglial cell cultures.
The effect of different eicosanoids on adenosine-3', 5'-cyclic-monophosphate (cAMP) accumulation in primary astroglial cell cultures prepared from newborn rat brain was studied. The stable prostacyclin-analogue, iloprost, effectively stimulated cAMP synthesis in a concentration-dependent, saturable manner, the EC50 being about 3 x 10(-8) M. Prostaglandin (PG) E2 was less potent, without reaching plateau even at 10(-5) M. Prostaglandins D2 and F2 alpha, and the stable thromboxane A2-analogue, U 46619, as well as leukotrienes (LT) B4, C4, D4 and E4 were not effective and did not attenuate basal or isoprenaline (10(-8) M)-stimulated astroglial cAMP formation. This is the first indication for the existence of a prostacyclin receptor coupled positively to the adenylate cyclase in astrocytes. Other eicosanoids are unlikely to be involved in receptor-mediated regulation of astroglial cAMP levels.
Seregi
A
A
Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest.
Schobert
A
A
Hertting
G
G
D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000378:metabolism*; D002317:Cardiovascular Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000096:biosynthesis*; D011464:Epoprostenol / Q000494:pharmacology*; D006801:Humans; D016285:Iloprost; D011453:Prostaglandins / Q000494:pharmacology; D051381:Rats; D013189:SRS-A / Q000494:pharmacology
D002317:Cardiovascular Agents; D011453:Prostaglandins; D013189:SRS-A; D011464:Epoprostenol; D000242:Cyclic AMP; D016285:Iloprost