Cleavage of specific sites of RNA by designed ribozymes.
Two ribozymes were designed for site-specific cleavage of RNA. A UA site in an undecaribonucleotide was cleaved by a ribozyme consisting of two partially paired oligoribonucleotides with chain lengths of 19 and 15. The other ribozyme, which consists of a 19-mer and a 13-mer, recognized a UC sequence at positions 42 and 43 of 5 S rRNA.
Koizumi
M
M
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Iwai
S
S
Ohtsuka
E
E
D001483:Base Sequence; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012313:RNA / Q000378:metabolism*; D012326:RNA Splicing; D016337:RNA, Catalytic; D012335:RNA, Ribosomal / Q000378:metabolism*; D012341:RNA, Ribosomal, 5S / Q000378:metabolism; D013379:Substrate Specificity
D016337:RNA, Catalytic; D012335:RNA, Ribosomal; D012341:RNA, Ribosomal, 5S; D012313:RNA