title: Cytotoxic T lymphocyte recognition of hybrid MHC class I molecules.
abstract: We have utilized a group of MHC class I genes produced by in vitro recombination between Dp and Dd to study recognition of MHC class I molecules by cytolytic T cells (CTLs). Both polyclonal allo-specific and H-2-restricted CTLs require that alpha 1 and alpha 2 of the target class I molecule be derived from the same haplotype for efficient killing. By using T-cell lines we showed that within the bulk population there must exist a fraction of T cells which can recognize epitopes in alpha 1 or alpha 2. Critical residues for T-cell recognition have been identified using these chimeric genes.
authors: Department of Microbiology and Immunology, University of North Carolina, Chapel Hill 27514.
authors: T
authors: T
authors: Kanda
authors: M J
authors: MJ
authors: Macchi
authors: K
authors: K
authors: LaPan
authors: M
authors: M
authors: Darsley
authors: E
authors: E
authors: Appella
authors: J A
authors: JA
authors: Frelinger
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000939:Epitopes; D005091:Exons; D005805:Genes, MHC Class I; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000276:immunology*; D006824:Hybridization, Genetic; D007694:Killer Cells, Natural / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D006183:H-2 Antigens; D015395:Histocompatibility Antigens Class I