Ultrastructural detection of herpes-type virions by negative staining in oral hairy leukoplakia.
Hairy leukoplakia (HL) is a newly recognized virus-associated lesion of oral mucosa that occurs in persons infected with human immunodeficiency virus. Studies have demonstrated Epstein-Barr virus within epithelial cells of HL. The authors examined 12 cases of HL by transmission electron microscopy to compare the use of a negative staining technique versus routine plastic embedment for the detection of viruses. Herpes-type virions were identified by both methods in 11 cases. One case had negative results for viruses by both methods. Negative staining is a simple and rapid technique that compares favorably with plastic embedment in the detection of herpes-type virions in HL.
Reed
K D
KD
Department of Pathology, Wilford Hall, United States Air Force Medical Center, Lackland Air Force Base, Texas 78236-5300.
Fowler
C B
CB
Brannon
R B
RB
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications*; D000328:Adult; D002175:Candida / Q000302:isolation & purification; D005260:Female; D006564:Herpesviridae / Q000302:isolation & purification*; D006801:Humans; D007972:Leukoplakia, Oral / Q000209:etiology / Q000382:microbiology* / Q000473:pathology; D008854:Microscopy, Electron; D008875:Middle Aged; D013194:Staining and Labeling; D014771:Virion / Q000302:isolation & purification