A type II keratin is expressed in glial cells of the goldfish visual pathway.
The predominant intermediate filament proteins of the goldfish visual pathway consist of neuronal and non-neuronal isoelectric variants (58 kd). We have isolated a cDNA clone for the glial intermediate filament protein (ON3) from an optic nerve expression library. The predicted amino acid sequence of this clone reveals that it codes for a type II keratin representing the goldfish equivalent of mammalian keratin K8. K8 has been shown to be associated with embryogenesis and development. Unlike the mammalian visual system, the goldfish visual pathway displays a remarkable capacity for functional regeneration. The expression of K8, a protein not usually expressed in glial cells but shown to be associated with development, in the goldfish optic nerve may be involved with the processes of growth and regeneration in the goldfish visual pathway.
Giordano
S
S
Department of Biochemistry, State University of New York at Stony Brook 11794.
Glasgow
E
E
Tesser
P
P
Schechter
N
N
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003530:Cyprinidae / Q000235:genetics*; D004247:DNA / Q000235:genetics; D006054:Goldfish / Q000235:genetics* / Q000502:physiology; D007633:Keratins / Q000032:analysis* / Q000235:genetics; D008322:Mammals / Q000235:genetics; D008969:Molecular Sequence Data; D009416:Nerve Regeneration; D009457:Neuroglia / Q000032:analysis*; D009900:Optic Nerve / Q000032:analysis* / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D014795:Visual Pathways / Q000032:analysis*
D012333:RNA, Messenger; D007633:Keratins; D004247:DNA