Presence of phospho-tyrosine in alkaline hydrolysate of pig skeletal muscle myosin.
A small part of the P-containing fraction of myosin hydrolysate which could be eluted with basic solvent (triethylamine) buffer was accumulated on chromatographic column. On the basis of the elution profile, P- and tyrosine content determination, moreover the thin layer chromatographic separation and specific Tyr reaction, this fraction is suggested to be phosphotyrosine. The concentration of P-Tyr is about 2 mol in pig muscle myosin calculated for 500 kDa protein. As the higher P-containing myosins also have an approx. 2 mol P-Tyr, it is thought that this concentration had been present in myosins already before alkaline hydrolysis.
Fazekas
S
S
Second Institute of Biochemistry, Semmelweis University Medical School, Budapest, Hungary.
Ováry
I
I
Székessy-Hermann
V
V
D000818:Animals; D002850:Chromatography, Gel; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009132:Muscles / Q000032:analysis*; D009218:Myosins / Q000032:analysis*; D019000:Phosphotyrosine; D013552:Swine; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis
D019000:Phosphotyrosine; D014443:Tyrosine; D009218:Myosins