The large neurofilament subunit (NF-H) of the rat: cDNA cloning and in situ detection.
A lambda gt11 expression library was screened with a polyclonal antiserum directed against Wla Wolfgram protein which corresponds to the 2',3'-cyclic nucleotide 3'-phosphohydrolase. This antiserum also recognizes epitopes of the high protein subunit of neurofilaments (NF-H). An NF-H cDNA clone (pNF-H1: 1.7 kb) was isolated and characterized. Using pNF-H1 as a probe, a second cDNA clone (pNF-H2: 3 kb) was isolated from the lambda gt11 library. The two clones were sequenced and pNF-H2 was found to encode 80% of rat NF-H protein (coil-2 and C-terminal region). The C-terminal region contains an unusual sequence with stretches of repeats of Lys-Ser-Pro which represent possible phosphorylation sites. Specific localization in neurons of the corresponding mRNA was demonstrated by in situ hybridization using the pNF-H1 as a probe.
Dautigny
A
A
Laboratoire des Protéines (UA 1188 CNRS), Université de Paris, France.
Pham-Dinh
D
D
Roussel
C
C
Felix
J M
JM
Nussbaum
J L
JL
Jollès
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D003001:Cloning, Molecular; D003599:Cytoskeleton / Q000032:analysis; D004247:DNA / Q000235:genetics*; D004262:DNA Restriction Enzymes; D007106:Immune Sera; D007382:Intermediate Filaments / Q000032:analysis; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009185:Myelin Proteins / Q000276:immunology; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000235:genetics; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D051381:Rats
D007106:Immune Sera; D046911:Macromolecular Substances; D009185:Myelin Proteins; D012333:RNA, Messenger; C028312:Wolfgram proteins; D011061:Poly A; D012313:RNA; D004247:DNA; D004262:DNA Restriction Enzymes