Transport rates of proteins in porous materials with known microgeometry.
Many biological and biotechnological systems involve the diffusion of macromolecules through complicated macroporous (pore size on the order of 10-100 microns) environments. In this report, we present and evaluate an experimental system for measuring the rate of protein transport in an inert, macroporous membrane. For this particular membrane system, the microgeometry was characterized in terms of distribution of pore size, position, and orientation. Although the rate of protein desorption was much less than expected based on continuum diffusion models, we demonstrate that the measured transport rates are consistent with diffusion of protein in a complex, interconnected network of water-filled pores. The porous systems exhibit transitional behavior in quantitative agreement with the behavior of percolation lattices (mean square error 7%, n = 29). Predictive mathematical models of the diffusion process were developed: these models used percolation concepts to describe pore topology, continuum models of diffusion/dissolution to describe protein movement at each single pore, and measured pore size distributions. Effective diffusion coefficients for protein transport in aqueous, constricted macropores were predicted by this technique. Predicted diffusion coefficients, based on measured and derived microstructural parameters, agree with experimentally measured diffusion coefficients within a factor of 2. This approach may be useful in the design of porous polymer systems for biological applications and for evaluating other biological systems where conduction of mass, heat, momentum, or charge occurs in a heterogeneous environment.
Saltzman
W M
WM
Harvard/MIT Division of Health Sciences and Technology, Cambridge, Massachusetts 02139.
Langer
R
R
D007700:Kinetics; D008433:Mathematics; D008567:Membranes, Artificial; D008962:Models, Theoretical; D011145:Polyvinyls; D011506:Proteins / Q000378:metabolism; D012710:Serum Albumin, Bovine; D005719:gamma-Globulins
D008567:Membranes, Artificial; D011145:Polyvinyls; D011506:Proteins; D005719:gamma-Globulins; C016438:ethylenevinylacetate copolymer; D012710:Serum Albumin, Bovine