Concomitant purification of prostatic carcinoma tumor markers from human seminal fluid under nondenaturing conditions.
We report a protocol for concomitant purification to homogeneity of both prostatic acid phosphatase [orthophosphoric-monoester phosphohydrolase (acid optimum), EC 3.1.3.2] and prostate-specific antigen, from human seminal fluid. The method requires only two chromatographic steps: passage through an Affigel-Blue column and gel filtration HPLC. This is a fast, efficient procedure for purification of these two important tumor markers, which are specific for prostatic cancer.
Rusciano
D
D
Cellular Biology Laboratory, Sclavo Research Center, Siena, Italy.
Berardi
A
A
Ceccarini
C
C
Terrana
B
B
D000135:Acid Phosphatase / Q000302:isolation & purification; D000951:Antigens, Neoplasm / Q000302:isolation & purification*; D014408:Biomarkers, Tumor / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D017430:Prostate-Specific Antigen; D012661:Semen / Q000032:analysis*
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D000135:Acid Phosphatase; D017430:Prostate-Specific Antigen