title: The morphology of simian immunodeficiency virus as shown by negative staining electron microscopy.
abstract: Negative staining electron microscopy was used to study sucrose gradient-purified preparations of the simian immunodeficiency virus (SIVmac251). Both isolated and aggregated virus particles were observed together with some free-lying virus cores. The cores were 110 nm long and 25 to 50 nm wide and were mainly conical or wedge-like in shape. Surface projections were seen on the envelope membrane of many of the virus particles; the knobs were approximately 6 nm in length, 10 nm wide and from an end-on view they had a Y or triangular-shaped morphology.
authors: National Institute for Biological Standards and Control, South Mimms, Hertfordshire, U.K.
authors: C
authors: C
authors: Grief
authors: D J
authors: DJ
authors: Hockley
authors: C E
authors: CE
authors: Fromholc
authors: P A
authors: PA
authors: Kitchin
mesh_terms: D002499:Centrifugation, Density Gradient; D008854:Microscopy, Electron / Q000379:methods; D009942:Organometallic Compounds; D010316:Particle Size; D015302:Simian Immunodeficiency Virus / Q000648:ultrastructure*; D013194:Staining and Labeling / Q000379:methods; D014759:Viral Envelope Proteins / Q000032:analysis; D014771:Virion / Q000648:ultrastructure
chemical_list: D009942:Organometallic Compounds; D014759:Viral Envelope Proteins; C005460:uranyl acetate