A N-acetyllactosamine-specific cell-binding activity in a plant pathogen, Erwinia rhapontici.
A strain of the phytopathogenic bacterial species, Erwinia rhapontici, was found to cause hemagglutination of human erythrocytes that was specifically inhibited by beta-galactosides. Of the monosaccharides tested, N-acetyl galactosamine and galactose efficiently inhibited the hemagglutination. The most potent inhibitor identified was Ga1 beta 1-4GlcNAc that was 30-100-fold more potent than Ga1 beta 1-3GlcNac or Ga1 beta 1-3GalNAc. Fetuin had no effect on the hemagglutination whereas asialofetuin was inhibitory. No blood group specificity was found for the hemagglutinin. These findings indicate that the E. rhapontici strain possesses a novel bacterial cell-binding activity with specificity for terminal N-acetyllactosamine residues.
Korhonen
T K
TK
Department of General Microbiology, University of Helsinki, Finland.
Haahtela
K
K
Pirkola
A
A
Parkkinen
J
J
D000116:Acetylgalactosamine / Q000494:pharmacology; D000117:Acetylglucosamine / Q000494:pharmacology; D000606:Amino Sugars / Q000378:metabolism*; D001089:Arabinose / Q000494:pharmacology; D001212:Asialoglycoproteins; D001422:Bacterial Adhesion; D004885:Erwinia / Q000378:metabolism*; D060748:Fetuins; D005690:Galactose / Q000494:pharmacology; D006384:Hemagglutination / Q000187:drug effects*; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006801:Humans; D009844:Oligosaccharides / Q000494:pharmacology; D000509:alpha-Fetoproteins / Q000494:pharmacology
D000606:Amino Sugars; D001212:Asialoglycoproteins; D060748:Fetuins; D009844:Oligosaccharides; D000509:alpha-Fetoproteins; C017029:asialofetuin; C000458:N-acetyllactosamine; D001089:Arabinose; D000116:Acetylgalactosamine; D000117:Acetylglucosamine; D005690:Galactose