Comparative study of in vitro CD1a and HLA-class I antigens endocytosis by human thymocytes and Langerhans cells.
Langerhans cells and thymocytes were comparatively studied for their monoclonal antibody-induced endocytic activity. The two cell types were CD1a or Class I-HLA immunolabeled, incubated under similar conditions and the effects induced on membrane mobility were analyzed by a fluorescence method for the thymocytes and at the ultrastructural level for both cell types. We provide evidence that thymocytes are able to cap and internalize by receptor-mediated endocytosis CD1a antigen and Class I-HLA, whereas Langerhans cells present only a process of internalization by receptor-mediated endocytosis for both membrane antigens.
Ray
A
A
INSERM U.209, Laboratoire de Recherche Dermatologique et Immunologie, Hôpital Ed. Herriot, Lyon, France.
Schmitt
D
D
Dezutter-Dambuyant
C
C
Hanau
D
D
Thivolet
J
J
D018949:Antigens, CD1; D000943:Antigens, Differentiation / Q000276:immunology*; D002478:Cells, Cultured; D004705:Endocytosis; D000078404:Epidermal Cells; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006801:Humans; D007801:Langerhans Cells / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D018949:Antigens, CD1; D000943:Antigens, Differentiation; D015395:Histocompatibility Antigens Class I