title: Erythromycin-induced stabilization of ermA messenger RNA in Staphylococcus aureus and Bacillus subtilis.
abstract: Erythromycin-induced stabilization of ermA mRNA was studied in Staphylococcus aureus, its original host background, and in Bacillus subtilis, subcloned on plasmid vectors. By RNA blot analysis it was shown that 40 nM-erythromycin specifically increased the chemical half-life of ermA mRNA from 2.5 to 17.5 minutes whereas the half-life of cat-86 mRNA was not increased by erythromycin. While expression of ermA has been shown to be induced by erythromycin at the level of translation, our studies with three ermA constitutive mutants demonstrated that mRNA stabilization in growing cells occurred independently of induced gene expression, suggesting that the stabilized mRNA was not functional for protein synthesis. Studies of ermA/lacZ fusions demonstrated that the 5' end of the mRNA was sufficient to confer stabilization. Translation of specific amino acid codons in a leader peptide located at the extreme 5' end of the mRNA was required for the erythromycin-induced stabilization as a frameshift mutation introduced into the leader peptide determinant abolished stabilization. By S1 mapping, no differences were detected in the length of the 5' or 3' end of ermA mRNA with the addition of erythromycin, indicating that the stabilized transcript was not processed at its ends.
authors: Department of Genetics, University of Wisconsin, Madison 53706.
authors: P
authors: P
authors: Sandler
authors: B
authors: B
authors: Weisblum
mesh_terms: D001412:Bacillus subtilis; D001483:Base Sequence; D004917:Erythromycin / Q000494:pharmacology*; D005798:Genes, Bacterial / Q000187:drug effects*; D006207:Half-Life; D007763:Lac Operon; D008969:Molecular Sequence Data; D009154:Mutation; D014176:Protein Biosynthesis; D012329:RNA, Bacterial / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D013211:Staphylococcus aureus
chemical_list: D012329:RNA, Bacterial; D012333:RNA, Messenger; D004917:Erythromycin