C-terminal angiogenin peptides inhibit the biological and enzymatic activities of angiogenin.
Synthetic peptides corresponding to the C-terminal region of angiogenin (Ang) inhibit the enzymatic and biological activities of the molecule while peptides from the N-terminal region do not affect either activity. The peptide Ang(108-121) transiently abolishes the inhibition of cell-free protein synthesis caused by angiogenin coincidentally with its cleavage of reticulocyte RNA. Several C-terminal peptides also inhibit nuclease activity of angiogenin when tRNA is the substrate. Furthermore, peptide Ang(108-123) significantly decreases neovascularization elicited by angiogenin in the chick chorioallantoic membrane assay.
Rybak
S M
SM
Center for Biochemical and Biophysical Sciences and Medicine, Harvard Medical School, Boston, Massachusetts 02115.
Auld
D S
DS
St Clair
D K
DK
Yao
Q Z
QZ
Fett
J W
JW
D000595:Amino Acid Sequence; D043925:Angiogenesis Inducing Agents / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D000818:Animals; D006224:Cricetinae; D006131:Growth Inhibitors; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000494:pharmacology*; D011500:Protein Synthesis Inhibitors / Q000037:antagonists & inhibitors; D011506:Proteins / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D012313:RNA / Q000378:metabolism; D011817:Rabbits; D012156:Reticulocytes / Q000187:drug effects / Q000378:metabolism; D012259:Ribonuclease, Pancreatic; D013379:Substrate Specificity
D043925:Angiogenesis Inducing Agents; D006131:Growth Inhibitors; D010446:Peptide Fragments; D011500:Protein Synthesis Inhibitors; D011506:Proteins; D012313:RNA; C046366:angiogenin; D012259:Ribonuclease, Pancreatic