title: Modulation of adrenergic neurotransmission in the rat tail artery by dietary lipids.
abstract: The effects of dietary lipids on prejunctional alpha 2-adrenoceptor function were investigated in perfused/superfused caudal arteries from adult rats. The investigation was designed to study the effects of diet supplemented with saturated fatty acids (coconut oil diet) or unsaturated fatty acids (sunflower oil diet) on alpha 2-adrenoceptor neuronal function in the proximal rat tail artery. Pregnant rats were fed Purina Rodent Chow (reference diet) or a semisynthetic diet containing 16% (wt/wt) of either sunflower oil or coconut oil. Neonatal pups were exposed to the diet via maternal milk and weaned rats were maintained on the same diet throughout adulthood. Artery segments (5-6 cm) were prelabeled with [3H]norepinephrine and perfused/superfused with Krebs-bicarbonate solution at 37 degrees C. The release of endogenous norepinephrine, total 3H, and [3H]norepinephrine was measured during field stimulation (supramaximal voltage, 5 Hz, 1 ms duration, for a total of 1,200 pulses). Both test diets caused a significant increase in norepinephrine content when compared with the reference diet (p less than 0.01). The results show that field stimulation-evoked release of norepinephrine from arteries obtained from rats fed coconut oil diet was significantly less than that exhibited by either the sunflower oil group or the reference group (p less than 0.05). Phentolamine (3 microM) caused a significant increase in percent release of endogenous norepinephrine and total 3H (p less than 0.05) in all groups. However, the increase above control values for the sunflower oil group was higher than the coconut oil group which suggested that dietary manipulations altered alpha2-adrenoceptor sensitivity.(ABSTRACT TRUNCATED AT 250 WORDS)
authors: Department of Pharmacology and Toxicology, School of Pharmacy, University of Kansas, Lawrence 66045.
authors: W E
authors: WE
authors: Semafuko
authors: C O
authors: CO
authors: Rutledge
authors: W R
authors: WR
authors: Dixon
mesh_terms: D000818:Animals; D001158:Arteries / Q000187:drug effects / Q000294:innervation; D004032:Diet; D004041:Dietary Fats / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D009638:Norepinephrine / Q000378:metabolism; D010646:Phentolamine / Q000494:pharmacology; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D011942:Receptors, Adrenergic, alpha / Q000187:drug effects / Q000502:physiology; D013564:Sympathetic Nervous System / Q000187:drug effects*; D009435:Synaptic Transmission / Q000187:drug effects*
chemical_list: D004041:Dietary Fats; D011942:Receptors, Adrenergic, alpha; D009638:Norepinephrine; D010646:Phentolamine