title: Caldesmon150, caldesmon77 and skeletal muscle troponin T share a common antigenic determinant.
abstract: Caldesmon and troponin are known to control actin-myosin interaction in smooth and striated muscle. We showed that polyclonal antibodies to smooth muscle caldesmon150 cross-react with non-muscle caldesmon77 and with skeletal muscle troponin T. The epitope recognized by caldesmon antibodies is located in the CB 2 peptide [residues 71-151] of troponin T. It is supposed that the observed cross-reactivity is due to the common structure of the tropomyosin-binding site of caldesmon and troponin T.
authors: Institute of Experimental Cardiology, USSR Cardiology Research Center, USSR Academy of Medical Sciences, Moscow.
authors: V P
authors: VP
authors: Shirinsky
authors: K G
authors: KG
authors: Biryukov
authors: A V
authors: AV
authors: Vorotnikov
authors: N B
authors: NB
authors: Gusev
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D002148:Calmodulin-Binding Proteins / Q000276:immunology*; D002417:Cattle; D002645:Chickens; D002918:Chymotrypsin; D003488:Cyanogen Bromide; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology*; D015151:Immunoblotting; D008970:Molecular Weight; D009130:Muscle, Smooth / Q000032:analysis; D009132:Muscles / Q000032:analysis*; D010446:Peptide Fragments / Q000276:immunology; D011817:Rabbits; D051381:Rats; D014336:Troponin / Q000276:immunology*; D020107:Troponin T
chemical_list: D002148:Calmodulin-Binding Proteins; D000939:Epitopes; D010446:Peptide Fragments; D014336:Troponin; D020107:Troponin T; D002918:Chymotrypsin; D003488:Cyanogen Bromide