Immunogenicity of peptide fusions to hepatitis B virus core antigen.
Several gene fusions have been constructed in which coding sequences for antigenic regions of the pre-S sequences of hepatitis B virus, hepatitis B surface antigen, and the envelope protein of human immunodeficiency virus were linked to the 3' end of that for the first 144 residues of hepatitis B core antigen. The sequences were expressed efficiently in Escherichia coli to give stable products that assembled to form particles morphologically similar to hepatitis B core antigen itself. The products exhibited the antigenic and immunogenic characteristics of both the hepatitis B core antigen epitopes and the epitopes carried by the additional sequences, thus illustrating the value of such proteins as immunological reagents and potential vaccines.
Stahl
S J
SJ
Biogen S.A., Geneva, Switzerland.
Murray
K
K
D000595:Amino Acid Sequence; D000918:Antibody Specificity; D003001:Cloning, Molecular; D000939:Epitopes / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D005814:Genes, Viral; D006512:Hepatitis B Core Antigens / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D006515:Hepatitis B virus / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000032:analysis
D000939:Epitopes; D006512:Hepatitis B Core Antigens; D011993:Recombinant Fusion Proteins