title: Lysozyme gene specific transcription in isolated hen oviduct nuclei.
abstract: 1. Transcription from the chicken lysozyme gene domain and the density of RNA polymerase molecules was studied by incubating isolated hen oviduct nuclei in an in vitro transcription system with [alpha-32P]UTP and hybridizing the purified [32P]RNA with immobilized restriction fragments. 2. The labeled RNA hybridized most prominently to coding and flanking restriction fragments that contain repetitive sequences. 3. Relatively weak hybridization signals with a coding, single-copy, genomic fragment and cDNA fragments were detected using 5000 microCi [alpha-32P]UTP per ml of incubation mixture. 4. Flanking, non-repetitive fragments did not hybridize to the [32P]RNA. 5. The results show that transcription from repetitive sequences dominates over lysozyme gene specific transcription.
authors: Institut für Physiologie Chemie, Universitäts-Krankenhaus Hamburg, F.R.G.
authors: J P
authors: JP
authors: von Kries
authors: W H
authors: WH
authors: Strätling
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002645:Chickens; D004262:DNA Restriction Enzymes; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005260:Female; D005796:Genes; D009113:Muramidase / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010057:Oviducts / Q000201:enzymology*; D011061:Poly A / Q000235:genetics; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D012321:DNA-Directed RNA Polymerases; D004262:DNA Restriction Enzymes; D009113:Muramidase