Bioassays for retinoic acid-like substances using cultured human keratinocytes.
Using cultured human keratinocytes, three bioassays for retinoic acid-like substances have been developed. They are based on the ability of these substances (1) to inhibit cross-linked envelope formation, (2) to inhibit the synthesis of the K1 keratin, and (3) to stimulate the synthesis of the K19 keratin. The data, expressed as 50% inhibitory or activating concentrations were used to rank compounds according to their activity.
Regnier
M
M
Centre International de Recherches Dermatologiques (CIRD), Sophia Antipolis, Valbonne, France.
Eustache
J
J
Shroot
B
B
Darmon
M
M
D001345:Autoradiography; D001681:Biological Assay; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism*; D007633:Keratins / Q000096:biosynthesis; D011506:Proteins / Q000032:analysis; D012995:Solubility; D014212:Tretinoin / Q000378:metabolism*
D011506:Proteins; D014212:Tretinoin; D007633:Keratins