Tartrate-resistant acid ATPase as a cytochemical marker for osteoclasts.
We present a modified histochemical method for staining osteoclasts and adjacent mononuclear cells which takes advantage of the recently described substrate specificity for ATP of osteoclastic acid phosphatase. Staining of osteoclasts using ATP as substrate exhibits by light microscopy the same tartrate resistance as conventional acidic phosphatases, without the bone surface staining seen with other substrates. This feature, coupled with specific staining of fewer vicinal mononuclear cells, makes this method potentially useful for studying osteoclast ontogeny and function.
Andersson
G N
GN
Department of Oral Pathology, Karolinska Institutet, Huddinge University Hospital, Sweden.
Marks
S C
SC
D000135:Acid Phosphatase / Q000032:analysis* / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000032:analysis* / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D006651:Histocytochemistry; D010010:Osteoclasts / Q000201:enzymology*; D051381:Rats; D013194:Staining and Labeling; D013644:Tartrates / Q000494:pharmacology*
D013644:Tartrates; D000255:Adenosine Triphosphate; D000135:Acid Phosphatase; D000251:Adenosine Triphosphatases; C029768:tartaric acid