[Direct detection in diarrheal stools of Escherichia coli adhesion factors CFA/I, CFA/II and E8775 by the india ink immune reaction].
An India ink immune reaction was used for the direct detection, in diarrhoeal stools, of Escherichia coli possessing the CFA/I, CFA/II and E8775 fimbriae. With this method, a presumptive diagnosis of enterotoxigenic E. coli (ETEC) can rapidly be made. Staining required the bivalency of rabbit anti-fimbriae IgG; the F(ab')2 fragment was necessary. The reaction was impossible with purified Fab fragment. A comparative study of this technique and detection by culture of ETEC strains showed good correlation when the India ink immune reaction was performed at the beginning of infection.
Germani
Y
Y
Unité de Bactériologie expérimentale, Institut Pasteur, Nouméa.
Bégaud
E
E
Guesdon
J L
JL
Moreau
J P
JP
D000942:Antigens, Bacterial / Q000032:analysis*; D002244:Carbon; D004396:Coloring Agents; D003968:Diarrhea, Infantile / Q000382:microbiology*; D004926:Escherichia coli / Q000276:immunology*; D005243:Feces / Q000032:analysis*; D036781:Fimbriae Proteins; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007159:Immunologic Tests; D007223:Infant; D013194:Staining and Labeling
D000942:Antigens, Bacterial; D004396:Coloring Agents; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; C028433:chinese ink; C022924:colonization factor antigens; D036781:Fimbriae Proteins; D002244:Carbon
Dépistage direct dans les selles diarrhéiques des Escherichia coli porteurs des adhésines CFA/I, CFA/II et E8775 par la réaction immuno/encre de Chine.