title: Mapping of monoclonal antibodies to human factor IX.
abstract: We used recombinant DNA techniques to map a panel of six monoclonal antibodies (MoAbs) to regions of the human factor IX molecule. A-2 maps to 17 amino acids at the amino terminus of the heavy chain of IXa; 2D5, an inhibitor of clotting, is defined to 36 amino acids of the first EGF-like domain of human factor IX. A-4, A-5, C10D, and FXC008 all map to a region of the heavy chain containing amino acids 180 through 310, suggesting an immunodominant site. FXC008 has been reported to interfere with binding of factor IXa to factor VIII:Ca.
authors: Department of Biology, University of North Carolina, Chapel Hill 27599-3280.
authors: D
authors: D
authors: Frazier
authors: K J
authors: KJ
authors: Smith
authors: W F
authors: WF
authors: Cheung
authors: J
authors: J
authors: Ware
authors: S W
authors: SW
authors: Lin
authors: A R
authors: AR
authors: Thompson
authors: H
authors: H
authors: Reisner
authors: S P
authors: SP
authors: Bajaj
authors: D W
authors: DW
authors: Stafford
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000915:Antibody Affinity; D001667:Binding, Competitive; D003001:Cloning, Molecular; D000939:Epitopes / Q000302:isolation & purification; D005164:Factor IX / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping; D010449:Peptide Mapping / Q000379:methods
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D005164:Factor IX