Recombinant IL-3 induces histamine release from human basophils.
Human rIL-3 induces histamine release from some human basophils, with cells from atopics responding to a greater extent than non-atopic donors. The dose response curves were highly variable. IL-3 was active on purified basophils and the release process was slower and required more calcium than anti-IgE. Removal of surface IgE from basophils rendered them unresponsive to IL-3. The response could be restored by passive sensitization of basophils with IgE+, IgE known to bind histamine-releasing factors, and not IgE-, IgE unreactive with histamine-releasing factors. Thus, IL-3 uncovers IgE heterogeneity. IL-3 does not, however, directly interact with IgE+. Rather, passive sensitization with IgE+ or stimulation of basophils with low concentrations of several secretagogues renders the cells sensitive to IL-3. IL-3 may well play a pro-inflammatory role by potentiating the effects of IgE+ or various secretagogues.
MacDonald
S M
SM
Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21239.
Schleimer
R P
RP
Kagey-Sobotka
A
A
Gillis
S
S
Lichtenstein
L M
LM
D000888:Antibodies, Anti-Idiotypic / Q000502:physiology; D001491:Basophils / Q000276:immunology* / Q000502:physiology; D014408:Biomarkers, Tumor; D003888:Desensitization, Immunologic; D004306:Dose-Response Relationship, Immunologic; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology / Q000502:physiology; D007377:Interleukin-3 / Q000494:pharmacology* / Q000502:physiology; D008222:Lymphokines / Q000502:physiology; D009240:N-Formylmethionine Leucyl-Phenylalanine; D011947:Receptors, Antigen, B-Cell / Q000502:physiology; D011994:Recombinant Proteins / Q000494:pharmacology*; D000090324:Tumor Protein, Translationally-Controlled 1
D000888:Antibodies, Anti-Idiotypic; D014408:Biomarkers, Tumor; D007377:Interleukin-3; D008222:Lymphokines; D011947:Receptors, Antigen, B-Cell; D011994:Recombinant Proteins; D000090324:Tumor Protein, Translationally-Controlled 1; C026408:anti-IgE antibodies; D007073:Immunoglobulin E; D009240:N-Formylmethionine Leucyl-Phenylalanine