title: Anion channels in giant liposomes made of endoplasmic reticulum vesicles from rat exocrine pancreas.
abstract: Using the method of dehydration and rehydration, rough endoplasmic reticulum (RER) vesicles, isolated by differential centrifugation, can be enlarged to giant liposomes with diameters ranging from 5 to 200 micron. Patch-clamp studies on these giant RER liposomes revealed the existence of a channel with a mean conductance of 260 +/- 7 pS (n = 23; 140 mmol/liter KCl on both sides). The channel is about four times more permeable for Cl- than for K+. Its activity is strongly voltage regulated. At low potentials (+/- 20 mV) the channel is predominantly in its open state with an open probability near 1.0, whereas it closes permanently at high positive and negative voltages (+/- 70 mV). The channel activity is not influenced by changing the free Ca2+ concentration from 1 mmol/liter to less than 10(-9) mol/liter on either side, and is also not affected by typical Cl- -channel blockers like diphenylamine-2-carboxylate (DPC, 1 mmol/liter) or 4-acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid (SITS, 1 mmol/liter). Another chloride channel with a single-channel conductance of 79 +/- 6 pS (n = 4) was less frequently observed. In the potential range of -80 to +40 mV this channel displayed no voltage-dependent gating. We assume that these anion channels are involved in the maintenance of electroneutrality during Ca2+ uptake in the RER.
authors: Max-Planck-Institut für Biophysik, Frankfurt/Main, Federal Republic of Germany.
authors: A
authors: A
authors: Schmid
authors: H
authors: H
authors: Gögelein
authors: T P
authors: TP
authors: Kemmer
authors: I
authors: I
authors: Schulz
mesh_terms: D000818:Animals; D000838:Anions; D002458:Cell Fractionation; D004721:Endoplasmic Reticulum / Q000648:ultrastructure*; D007425:Intracellular Membranes / Q000648:ultrastructure*; D007473:Ion Channels / Q000032:analysis*; D008081:Liposomes / Q000032:analysis*; D010179:Pancreas / Q000648:ultrastructure*; D051381:Rats
chemical_list: D000838:Anions; D007473:Ion Channels; D008081:Liposomes