title: Enhancement of plasminogen activator activity in cultured endothelial cells by granulocyte colony-stimulating factor.
abstract: A hitherto unknown function of granulocyte colony-stimulating factor (G-CSF) was found using cultured endothelial cells. G-CSF stimulated activity of plasminogen activator (PA) in both extracellular and intracellular milieus of endothelial cells obtained from bovine carotid and aortic artery. This effect was dependent on the concentration of G-CSF added to the culture medium and on the treatment time. The extracellular activity was enhanced approximately 5-fold at a concentration of 5,000 colony-forming unit (CFU)/ml (2.6 nM) and in about a 15-hr treatment period. Analyses by fibrin and reverse fibrin autography revealed that activity of PA was much more increased than that of PA inhibitor in endothelial cells treated with G-CSF.
authors: Department of Biological Sciences, Tokyo Institute of Technology, Japan.
authors: S
authors: S
authors: Kojima
authors: H
authors: H
authors: Tadenuma
authors: Y
authors: Y
authors: Inada
authors: Y
authors: Y
authors: Saito
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000187:drug effects*; D005337:Fibrin / Q000032:analysis; D005342:Fibrinolysis; D016179:Granulocyte Colony-Stimulating Factor; D010960:Plasminogen Activators / Q000378:metabolism*; D014801:Vitamin A / Q000494:pharmacology
chemical_list: D003115:Colony-Stimulating Factors; D014801:Vitamin A; D016179:Granulocyte Colony-Stimulating Factor; D005337:Fibrin; D010960:Plasminogen Activators