[Etiological diagnosis of cryptosporidiosis].
1,014 faecal specimens were collected from 4 hospitals in Nanjing to investigate the infection of Cryptosporidium. Each faecal specimen was smeared and screened by auramine phenol staining method for oocyst, the positive and suspected specimens were identified by modified acid-fast staining method and safranin-methylene blue staining method. The result showed that 13 specimens were positive by auramine-phenol staining method and confirmed by modified acid-fast staining method. 5 suspected specimens were negative as shown by the latter two methods. The best result could be obtained when auramine-phenol staining method was first used and followed by modified acid-fast staining method. This technique is simple, sensitive and reliable. It is difficult to find oocysts by safranin methylene blue staining method when the oocysts are few.
Ha
F
F
Xu
S
S
D000818:Animals; D003457:Cryptosporidiosis / Q000175:diagnosis* / Q000469:parasitology; D003458:Cryptosporidium / Q000302:isolation & purification; D004751:Enteritis / Q000175:diagnosis; D005243:Feces / Q000469:parasitology; D006801:Humans; D007411:Intestinal Diseases, Parasitic / Q000175:diagnosis; D013194:Staining and Labeling / Q000379:methods