Unmasking of surface negativity on day 6 pregnant rat uterine epithelial cells by trypsin and pronase.
Cationic Ferritin and Ruthenium Red were used to label rat uterine luminal epithelial cells at estrus and 6th d of pregnancy. Labelling was heavy at estrus and light on 6th d. Trypsin and pronase reduced labelling at estrus and restored labelling at 6th d suggesting that negatively charged carbohydrates may become masked at the time of implantation.
Hosie
M J
MJ
Department of Histology and Embryology, University of Sydney, Australia.
Murphy
C R
CR
D000818:Animals; D000838:Anions; D002241:Carbohydrates / Q000032:analysis*; D004848:Epithelium / Q000032:analysis; D005260:Female; D005293:Ferritins; D006651:Histocytochemistry; D008854:Microscopy, Electron; D011247:Pregnancy; D011270:Pregnancy, Animal; D011402:Pronase / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012430:Ruthenium Red; D013194:Staining and Labeling; D014357:Trypsin / Q000494:pharmacology*; D014599:Uterus / Q000032:analysis* / Q000187:drug effects
D000838:Anions; D002241:Carbohydrates; C010297:polycationic ferritin; D012430:Ruthenium Red; D005293:Ferritins; D014357:Trypsin; D011402:Pronase