Human immune response against timothy grass pollen allergens. I. Shared idiotypes on human and murine antigen-B-specific lymphocyte receptors.
Enzyme-linked immunoassays were used to examine the binding specificity of affinity-purified anti-idiotypic antibody [against the idiotypic determinant on timothy grass pollen antigen B (AgB)-specific IgE] with murine AgB-specific IgG and IgE antibodies, and the serum from timothy-sensitive patients. In addition, the proliferative response of peripheral blood lymphocytes from timothy-sensitive patients with either antigen or anti-idiotypic antibody was examined. These studies suggest that the idiotypic determinant expressed on murine AgB-specific IgE is highly conserved on human AgB-specific IgE and T cells of timothy-sensitive patients.
Malley
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Oregon Regional Primate Research Center, Beaverton.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000502:physiology; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D001667:Binding, Competitive; D002475:Cellobiose / Q000494:pharmacology; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D007130:Immunoglobulin Idiotypes / Q000276:immunology* / Q000378:metabolism; D008213:Lymphocyte Activation; D051379:Mice; D006109:Poaceae / Q000276:immunology; D011058:Pollen / Q000276:immunology*; D011817:Rabbits; D011947:Receptors, Antigen, B-Cell / Q000187:drug effects / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000187:drug effects / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000939:Epitopes; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D011947:Receptors, Antigen, B-Cell; D011948:Receptors, Antigen, T-Cell; D002475:Cellobiose; D007073:Immunoglobulin E