Properties and characterization of a rat spleen cell-derived factor that induces resistance to natural killer cell lysis in YAC lymphoma cells.
Supernatants of Con A-stimulated rat spleen cell cultures contain a factor that induces relative resistance to NK cell-mediated cytotoxicity in the YAC cell line, a line that is otherwise highly susceptible to murine NK cell-mediated lysis. This NK-lysis resistance-inducing factor (LRIF) has a Mr of 12,600 Da, as determined by gel filtration chromatography, and an isoelectric pH of 4.8. NK-LRIF is heat labile and is de-activated by treatment with proteolytic enzymes. Unlike immune-IFN (IFN-gamma), NK-LRIF is not inactivated by pH 2 treatment, and antibodies capable of neutralizing IFN-alpha and IFN-gamma do not abrogate the effect of NK-LRIF. Highly purified IL-2 preparations lack NK-LRIF activity. NK-LRIF does not induce a general resistance to lysis in YAC cells, because control and NK-LRIF-treated YAC cells were equally susceptible to alloimmune cytotoxic T cells. YAC cells treated with NK-LRIF showed a marked enhancement (5- to 10-fold) in the expression of class I MHC Ag. This observation supports the proposition that the NK susceptibility of target cells could be inversely related to the expression of class I MHC Ag.
Saxena
R K
RK
Clinical Immunology Section, National Institute on Aging, Baltimore, Maryland 21224.
Saxena
Q B
QB
Adler
W H
WH
D000818:Animals; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D005260:Female; D006183:H-2 Antigens / Q000378:metabolism; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007113:Immunity, Innate; D007372:Interferons / Q000502:physiology; D007525:Isoelectric Focusing; D007694:Killer Cells, Natural / Q000276:immunology*; D008222:Lymphokines / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D008223:Lymphoma / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010447:Peptide Hydrolases; D051381:Rats; D011916:Rats, Inbred F344; D013154:Spleen / Q000166:cytology*
D006183:H-2 Antigens; D008222:Lymphokines; C021649:concanavalin A-induced helper factors; D007372:Interferons; D010447:Peptide Hydrolases