Analysis of chromatin pattern in blood lymphocytes of healthy donors and in lymphoid cells of patients with chronic lymphocytic leukaemia.
The optical Fourier transformation was used to analyse the chromatin/interchromatin pattern of lymphocytes of healthy subjects and lymphoid cells of patients with chronic lymphocytic leukaemia (CLL, type B, stage O). Peripheral blood smears were prepared routinely, fixed, and stained by the Feulgen method, and the photographic images of the nuclei were quantitatively analysed. From the radial distribution of light intensity of diffractograms, several Feulgen chromatin (F-chromatin/interchromatin) descriptors were evaluated. Four showed the strongest discriminant power and these descriptors discriminated well between lymphocytes of healthy donors and lymphoid cells of CLL patients, although F-chromatin/interchromatin components of the same sizes were found in lymphocytes and lymphoid cells.
Rozycka
M
M
Department of Histology and Embryology, Medical School, Warsaw, Poland.
Sawicki
W
W
Traczyk
Z
Z
Bem
W
W
Strojny
P
P
D000328:Adult; D002843:Chromatin / Q000648:ultrastructure*; D005583:Fourier Analysis; D006801:Humans; D007945:Leukemia, Lymphoid / Q000473:pathology*; D008214:Lymphocytes / Q000648:ultrastructure*; D055095:Optics and Photonics; D013194:Staining and Labeling
D002843:Chromatin