Epitope mapping of monoclonal antibodies against human immunodeficiency virus type 1 structural proteins by using peptides.
Murine monoclonal antibodies directed against the structural proteins p17 and p24 of human immunodeficiency virus type 1 were investigated in an epitope mapping system. Overlapping peptides consisting of 15 amino acids of the p17 and p24 protein, respectively, were used as competitors in an enzyme-linked immunosorbent assay. Three different immunogenic regions (A, B, and C) could be defined, one on p17 and two on p24. Twenty monoclonal antibodies reacted with the human immunodeficiency virus type 1 peptides of region B, although differences in the reactivity of these antibodies with human immunodeficiency virus type 2 and simian immunodeficiency virus strain mac were detectable. Recognized epitopes were characterized by computer analysis as described by T.P. Hopp and K.R. Woods (Proc. Natl. Acad. Sci. USA 78:3824-3828, 1981) and P.Y. Chou and G.D. Fasman (Biochemistry 13:222-245, 1974).
Niedrig
M
M
Robert Koch-Institut des Bundesgesundheitsamtes, Berlin, Federal Republic of Germany.
Hinkula
J
J
Weigelt
W
W
L'age-Stehr
J
J
Pauli
G
G
Rosen
J
J
Wahren
B
B
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis*; D015683:Gene Products, gag; D015483:HIV Antibodies / Q000276:immunology; D015488:HIV Antigens / Q000032:analysis* / Q000276:immunology*; D016655:HIV Core Protein p24; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D012191:Retroviridae Proteins / Q000276:immunology*; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus
D000911:Antibodies, Monoclonal; D000939:Epitopes; D015683:Gene Products, gag; D015483:HIV Antibodies; D015488:HIV Antigens; D016655:HIV Core Protein p24; D012191:Retroviridae Proteins; D014764:Viral Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; C054858:p17 protein, Human Immunodeficiency Virus Type 1