Increased sensitivity for Coomassie staining of sodium dodecyl sulfate-polyacrylamide gels using PhastSystem Development Unit.
Staining of proteins in PhastGel gradient media with Coomassie Blue R 350 was considerably improved using a lower concentration of methanol (10% v/v) and 2% ammonium sulfate in the staining solution and 10% acetic acid for destaining. The detection limit in sodium dodecyl sulfate-polyacrylamide gels was lowered by a factor of 10 to about 2 ng per protein band. The Coomassie staining method was adapted to the newly developed silver staining procedure so that both can be used in parallel in PhastSystem.
Heukeshoven
J
J
Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Universität, Hamburg, Federal Republic of Germany.
Dernick
R
R
D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation*; D011506:Proteins / Q000032:analysis; D012394:Rosaniline Dyes; D013194:Staining and Labeling / Q000295:instrumentation
D011506:Proteins; D012394:Rosaniline Dyes; C004692:coomassie Brilliant Blue