Susceptibility of EBV-carrying B cell lines to infection by HIV-1: variability of production of progeny virus and expression of viral antigens.
We have examined 3 different EBV-carrying B cell lines, in terms of ability to be super-infected by the human immunodeficiency virus (HIV-1), and have followed these lines, after infection by HIV-1, over a period of 3 months. We found significant variation among different HIV-1 strains in terms of the multiplicity of infection required to initiate infection in these EBV-positive cell lines. Persistent infection by HIV-1 in the absence of detectable cytopathic effects could be demonstrated, as evaluated by a variety of techniques, including reverse transcriptase assay and immunofluorescence. The results indicate also that all of these cell lines produced progeny HIV-1 intermittently, with large amounts of virus production on some days but not others. In contrast, they were all able to continuously express p24.
Tremblay
M
M
Lady Davis Institute for Medical Research of the Sir Mortimer B. Davis-Jewish General Hospital, Montreal, Quebec, Canada.
Wainberg
M A
MA
D001402:B-Lymphocytes / Q000382:microbiology*; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D015488:HIV Antigens / Q000032:analysis; D015497:HIV-1 / Q000254:growth & development*; D004854:Herpesvirus 4, Human; D006801:Humans; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D014779:Virus Replication
D015488:HIV Antigens; D012194:RNA-Directed DNA Polymerase