title: Structures of two kinds of mRNA encoding the chum salmon melanin-concentrating hormone.
abstract: The structures of two kinds of melanin-concentrating hormone (MCH) cDNA clones isolated from a chum salmon hypothalamus cDNA library were described. The MCH heptadecapeptide was present at the C terminus of a putative MCH precursor consisting of 132 amino acid residues. The two clones were 80% homologous with each other at the amino acid sequence level. Two genes, each directing one of the mRNAs was noted at about a single copy per haploid salmon genome. MCH genes were efficiently expressed as 0.9-kb poly(A)+RNA in salmon hypothalamus, and sequences hybridizable with salmon MCH cDNA were found in rat hypothalamus.
authors: Department of Molecular Biology, School of Medicine, Kitasato University, Kanagawa, Japan.
authors: M
authors: M
authors: Ono
authors: C
authors: C
authors: Wada
authors: I
authors: I
authors: Oikawa
authors: I
authors: I
authors: Kawazoe
authors: H
authors: H
authors: Kawauchi
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D007028:Hypothalamic Hormones; D008543:Melanins / Q000235:genetics*; D008547:Melanophores; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010907:Pituitary Hormones / Q000235:genetics*; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D012487:Salmonidae / Q000235:genetics*
chemical_list: D007028:Hypothalamic Hormones; D008543:Melanins; D010907:Pituitary Hormones; D012333:RNA, Messenger; D012313:RNA; C019463:melanin-concentrating hormone; D004247:DNA