title: Substance P stimulates translocation of protein kinase C in brain microvessels.
abstract: The action of substance P on protein kinase C in cerebral microvessels, isolated from bovine, was investigated. We found that in untreated microvessels 85% of the total activity was localized to the cytosolic fraction. Substance P caused a shift of activity to the membrane fraction, accompanied by a loss of activity in the cytosolic fraction. This effect resulted in dose-dependence and it was evident after 5 min treatment. These data suggest that substance P may be involved in the regulation of processes underlying protein phosphorylation in the blood-brain barrier.
authors: Departamento de Biología Molecular, Universidad Autónoma de Madrid, Spain.
authors: R E
authors: RE
authors: Catalán
authors: A M
authors: AM
authors: Martínez
authors: M D
authors: MD
authors: Aragonés
authors: I
authors: I
authors: Fernández
mesh_terms: D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000201:enzymology; D002560:Cerebrovascular Circulation; D003600:Cytosol / Q000201:enzymology; D007700:Kinetics; D008833:Microcirculation / Q000201:enzymology*; D011493:Protein Kinase C / Q000378:metabolism*; D013373:Substance P / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D013373:Substance P; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate