Coumarin-phalloidin: a new actin probe permitting triple immunofluorescence microscopy of the cytoskeleton.
7-Diethylamino-3-(4-isothiocyanotophenyl)-4-methylcoumarin (CPITC) was coupled to amino-methyldithiolanophalloidin to produce a new phalloidin derivative, coumarin-phalloidin, fluorescent in the blue region of the spectrum. Coumarin-phalloidin binds to actin with around 100-fold less affinity than unconjugated phalloidin, but with enough avidity to make it a useful stain for actin filaments. Appropriate filter combinations permit triple immunofluorescence microscopy of the cytoskeleton with fluorescein and rhodamine conjugates together with coumarin-phalloidin.
Small
J V
JV
Institute of Molecular Biology, Austrain Academy of Sciences, Salzburg.
Zobeley
S
S
Rinnerthaler
G
G
Faulstich
H
H
D000199:Actins / Q000378:metabolism; D003374:Coumarins / Q000378:metabolism; D003599:Cytoskeleton / Q000648:ultrastructure*; D005347:Fibroblasts / Q000378:metabolism / Q000648:ultrastructure*; D006801:Humans; D008856:Microscopy, Fluorescence; D009842:Oligopeptides / Q000378:metabolism; D010590:Phalloidine / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*
D000199:Actins; D003374:Coumarins; D009842:Oligopeptides; D010590:Phalloidine; C030123:coumarin