Cohesive properties of terminally differentiated keratinocytes.
The stratum corneum can be dissociated into single squames by several extraction methods: mechanically, with organic solvents, and with detergents. We have performed studied of reaggregation from single squames prepared by these methods. Mechanically dispersed corneocytes recombined into a lamellar-like structure closely resembling intact stratum corneum. Squames obtained by dissociation in ether formed a multilayered structure which differed from the intact tissue in the collapse of the bilayered lamellar structure into a single lucent band with osmiophilic deposits. Squames obtained after extraction with detergent failed to reaggregate.
Brysk
M M
MM
Department of Dermatology, University of Texas Medical Branch, Galveston.
Rajaraman
S
S
Penn
P
P
Barlow
E
E
Bell
T
T
D002448:Cell Adhesion / Q000187:drug effects; D002469:Cell Separation / Q000379:methods; D003902:Detergents / Q000494:pharmacology; D000078404:Epidermal Cells; D004817:Epidermis / Q000032:analysis / Q000187:drug effects; D006652:Histological Techniques; D006801:Humans; D007633:Keratins / Q000032:analysis*; D012997:Solvents / Q000494:pharmacology; D013314:Stress, Mechanical
D003902:Detergents; D012997:Solvents; D007633:Keratins