title: The Leishmania kinetoplast-mitochondrion contains terminal uridylyltransferase and RNA ligase activities.
abstract: Purified mitochondria of Leishmania tarentolae contain 3'-terminal uridylyltransferase and RNA ligase activities which can be solubilized by detergent lysis of the organelle. Run-off transcription of maxicircle and minicircle DNA also occurs in intact and Triton-lysed mitochondria, using [32P]GTP as the labeled precursor. Heparin inhibits the solubilized terminal uridylyltransferase activity but does not affect the labeling of endogenous RNAs in intact mitochondria with [32P]UTP. Clarification of the mitochondrial Triton lysate causes an increase in terminal uridylyltransferase activity with exogenous substrates. These two activities are candidates for involvement in a post-transcriptional RNA editing process of mitochondrial transcripts.
authors: Department of Biology, University of California, Los Angeles 90024.
authors: N
authors: N
authors: Bakalara
authors: A M
authors: AM
authors: Simpson
authors: L
authors: L
authors: Simpson
mesh_terms: D000818:Animals; D015342:DNA Probes; D004270:DNA, Circular / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D006493:Heparin / Q000494:pharmacology; D007891:Leishmania / Q000648:ultrastructure*; D008928:Mitochondria / Q000201:enzymology*; D009693:Nucleic Acid Hybridization; D009711:Nucleotides / Q000494:pharmacology; D009713:Nucleotidyltransferases / Q000378:metabolism*; D017830:Octoxynol; D011092:Polyethylene Glycols; D011118:Polynucleotide Ligases / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D011130:RNA Ligase (ATP) / Q000378:metabolism*; D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D012995:Solubility; D014158:Transcription, Genetic; D006599:UDPglucose-Hexose-1-Phosphate Uridylyltransferase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014544:Uridine Triphosphate / Q000378:metabolism
chemical_list: D015342:DNA Probes; D004270:DNA, Circular; D009711:Nucleotides; D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal; D011092:Polyethylene Glycols; D012313:RNA; D006160:Guanosine Triphosphate; D017830:Octoxynol; D006493:Heparin; D012343:RNA, Transfer; D009713:Nucleotidyltransferases; D006599:UDPglucose-Hexose-1-Phosphate Uridylyltransferase; D011118:Polynucleotide Ligases; D011130:RNA Ligase (ATP); D014544:Uridine Triphosphate