Determination of monosaccharides in glycoproteins by reversed-phase high-performance liquid chromatography on 2.1-mm narrowbore columns.
The monosaccharide composition of glycoproteins was determined by using a Supelcosil LC-18 150 x 2.1 mm narrowbore column under reversed-phase conditions. Methodology was developed for sample hydrolysis, N-acetylation, benzoylation, and cleanup prior to high-performance liquid chromatography. Monosaccharides were identified and quantified at picomole levels. Various glycoproteins were then analyzed, including ribonucleases A and B, Mucin, and fetuin. In addition, the monosaccharide content of Chinese hamster ovary cell-expressed recombinant human renin was determined.
Gisch
D J
DJ
Biotechnology Research, Upjohn Company, Kalamazoo, MI 49001.
Pearson
J D
JD
D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D006023:Glycoproteins / Q000032:analysis*; D009005:Monosaccharides / Q000032:analysis*; D009077:Mucins / Q000032:analysis; D009844:Oligosaccharides / Q000032:analysis; D012083:Renin / Q000032:analysis; D000509:alpha-Fetoproteins / Q000032:analysis
D006023:Glycoproteins; D009005:Monosaccharides; D009077:Mucins; D009844:Oligosaccharides; D000509:alpha-Fetoproteins; D012083:Renin