Microwave fixation provides excellent preservation of tissue, cells and antigens for light and electron microscopy.
It was demonstrated that microwave energy used simultaneously in combination with low concentrations of glutaraldehyde (0.05%) and formaldehyde (2.0%) rapidly preserved light microscopic histology and excellent fine structural details, as well as a variety of cytoplasmic and membrane-bound antigens. Specimen blocks up to 1 cm3 can be fixed in as brief a time as 26 ms using a specially designed microwave device (ultrafast microwave fixation method). The fast microwave fixation method, using a commercially available device, was successfully used to preserve granule-bound rat mast cell chymase which was subsequently detected by a postembedding immunogold procedure. Control of the following parameters is important to the microwave fixation method: (1) specimens with one dimension less than 1 cm; (2) irradiation temperatures lower than 50 degrees C; (3) irradiation times less than 50 s; (4) immediate replacement of the postirradiation solution with cold storage buffer; (5) fixing the specimen within 15 min after it is removed from its blood supply.
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G R
GR
Department of Pathology, Beth Israel Hospital, Boston, Massachusetts 02215.
Dvorak
A M
AM
D000818:Animals; D000941:Antigens / Q000032:analysis*; D005260:Female; D006652:Histological Techniques; D006801:Humans; D007124:Immunoenzyme Techniques; D008099:Liver / Q000648:ultrastructure; D008297:Male; D008407:Mast Cells / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000379:methods; D008872:Microwaves; D009206:Myocardium / Q000648:ultrastructure; D012867:Skin / Q000166:cytology; D013194:Staining and Labeling; D014021:Tissue Preservation / Q000379:methods
D000941:Antigens