A new monoclonal antibody recognizing the amino-terminal consensus sequence of vertebrate intermediate filament proteins.
The mouse monoclonal antibody ME 101 raised against human peripherin, an intermediate filament protein (IFP) specific to well defined neuronal populations, recognizes all the major classes of vertebrate IFP in immunoblotting assays. Desmin, GFAP, vimentin, peripherin and the lightest neurofilament protein (NF-L) were cleaved into carboxy- and amino-terminal halves by N-chlorosuccinimide at their unique trytophan residue. Whereas the antibody directed against the epitope common to every IFP (intermediate filament antigen or IFA) and located on the carboxy-terminal end of the rod domain recognizes the carboxy-terminal half, the ME 101 antibody, as the present study illustrates, recognizes specifically the amino-terminal half. From the amino acid sequence data of IFP, it is deduced that the cognate epitope is localized on the amino-terminal part of coil la.
Escurat
M
M
Collège de France, Laboratoire de Biochimie Cellulaire, Paris, France.
Phamgia
H
H
Huc
C
C
Pouplard-Barthelaix
A
A
Boitard
C
C
Bach
J F
JF
Gros
F
F
Portier
M M
MM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D000939:Epitopes; D006801:Humans; D006825:Hybridomas; D007381:Intermediate Filament Proteins / Q000276:immunology*; D008562:Membrane Glycoproteins; D051379:Mice; D009419:Nerve Tissue Proteins; D064531:Peripherins; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007381:Intermediate Filament Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; C578994:PRPH protein, human; D064531:Peripherins