Cytochemical studies on induced autophagocytosis in mouse exocrine pancreas.
1. The origin of the limiting membranes of autophagic vacuoles (AVs) in mouse pancreatic acinar cells was studied in vinblastine-induced autophagocytosis. 2. The marker enzymes used were adenosine triphosphatase, lipase, inosine diphosphatase and thiamine pyrophosphatase. The following impregnation techniques were used: unbuffered osmium tetroxide impregnation, imidazole-buffered osmium tetroxide impregnation and uranyl-lead-copper impregnation. 3. Only a weak lipase activity was observed between the limiting membranes of a few AVs. The AV membranes were stained heavily with all impregnation techniques used. 4. The origin of AV membranes seems to be same in mouse liver and exocrine pancreas in vinblastine-induced autophagocytosis.
Reunanen
H
H
Department of Cell Biology, University of Jyväskylä, Finland.
Hirsimäki
P
P
Punnonen
E L
EL
D017766:Acid Anhydride Hydrolases; D000251:Adenosine Triphosphatases / Q000032:analysis; D000818:Animals; D001343:Autophagy / Q000187:drug effects; D007425:Intracellular Membranes / Q000201:enzymology; D008049:Lipase / Q000032:analysis; D008297:Male; D051379:Mice; D009940:Organoids / Q000648:ultrastructure*; D009993:Osmium Tetroxide; D010179:Pancreas / Q000201:enzymology / Q000648:ultrastructure*; D010587:Phagocytosis / Q000187:drug effects; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis; D013194:Staining and Labeling; D013834:Thiamine Pyrophosphatase / Q000032:analysis; D014617:Vacuoles / Q000201:enzymology / Q000648:ultrastructure*; D014747:Vinblastine / Q000494:pharmacology
D014747:Vinblastine; D008049:Lipase; D010744:Phosphoric Monoester Hydrolases; D017766:Acid Anhydride Hydrolases; D000251:Adenosine Triphosphatases; D013834:Thiamine Pyrophosphatase; C021446:inosine diphosphatase; D009993:Osmium Tetroxide