Production and ELISA application of bispecific monoclonal antibodies against fluorescein isothiocyanate (FITC) and horseradish peroxidase (HRP).
Hybrid hybridomas producing bispecific monoclonal antibodies reacting with both horseradish peroxidase (HRP) and fluorescein isothiocyanate (FITC) were obtained by fusing two hybridoma lines and selecting the fused cells using a fluorescence activated cell sorter (FACS). FITC was used to label different monoclonal antibodies and the bispecific antibodies acted as a linking agent between FITC-labelled antibody and the marker enzyme HRP. This system was used in enzyme immunoassays for the detection of different antigens. The results suggest a wide application of bispecific anti-FITC/anti-HRP antibodies as a detection system in EIA.
Karawajew
L
L
Central Institute of Molecular Biology, Academy of Sciences of the GDR, Berlin.
Behrsing
O
O
Kaiser
G
G
Micheel
B
B
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D002459:Cell Fusion; D006063:Chorionic Gonadotropin / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins / Q000276:immunology*; D006735:Horseradish Peroxidase / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D007202:Indicators and Reagents; D051379:Mice; D008807:Mice, Inbred BALB C; D010544:Peroxidases / Q000276:immunology*; D013861:Thiocyanates / Q000276:immunology*; D000509:alpha-Fetoproteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D006063:Chorionic Gonadotropin; D005452:Fluoresceins; D007202:Indicators and Reagents; D013861:Thiocyanates; D000509:alpha-Fetoproteins; D006735:Horseradish Peroxidase; D010544:Peroxidases; D016650:Fluorescein-5-isothiocyanate