title: Function of the mammalian La protein: evidence for its action in transcription termination by RNA polymerase III.
abstract: We have tested the hypothesis that the mammalian La protein, which appears to be required for accurate and efficient RNA polymerase III transcription, is a transcription termination factor. Our data suggest that 3' foreshortened transcripts generated in La's absence are components of a novel transcription intermediate containing a paused polymerase. These transcripts are produced by fractionated transcription complexes, are synthesized with kinetics different from full-length transcripts, and are chasable to completion from the stalled transcription complexes. Together, these findings argue that termination by RNA polymerase III requires auxilliary factor(s) and implicate La as such a factor. Since La appears to facilitate transcript completion and release and also binds the resulting RNA product, it may be a regulator of RNA polymerase III transcription.
authors: Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06510.
authors: E
authors: E
authors: Gottlieb
authors: J A
authors: JA
authors: Steitz
mesh_terms: D001324:Autoantigens; D002478:Cells, Cultured; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D006801:Humans; D007700:Kinetics; D008954:Models, Biological; D012313:RNA / Q000378:metabolism; D012320:RNA Polymerase III / Q000235:genetics*; D012261:Ribonucleoproteins; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D000097585:SS-B Antigen
chemical_list: D001324:Autoantigens; D012321:DNA-Directed RNA Polymerases; D012313:RNA; D012320:RNA Polymerase III; D012261:Ribonucleoproteins; D014157:Transcription Factors; D000097585:SS-B Antigen