Immunoblot fingerprinting Aspergillus fumigatus.
A new technique for typing Aspergillus fumigatus is presented. This is based on immunoblot fingerprinting each isolate against a rabbit hyperimmune antiserum raised against A. fumigatus NCTC 2109. All isolates were typable and reproducibility for the 16 antigenic bands which formed the basis of the system was excellent. Discrimination was better than silver staining and revealed 11 types among the 21 isolates from eight patients with an aspergilloma. Each aspergilloma could be due to either a single or multiple types.
Burnie
J P
JP
Department of Medical Microbiology, St. Bartholomew's Hospital, London, U.K.
Matthews
R C
RC
Clark
I
I
Milne
L J
LJ
D000818:Animals; D000946:Antigens, Fungal / Q000032:analysis; D001228:Aspergillosis / Q000175:diagnosis; D001232:Aspergillus fumigatus / Q000032:analysis / Q000145:classification* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D008970:Molecular Weight; D011817:Rabbits; D015203:Reproducibility of Results; D012835:Silver Nitrate; D013194:Staining and Labeling
D000946:Antigens, Fungal; D012835:Silver Nitrate