Expression of CD1 antigens by peripheral blood mononuclear cells from hepatitis B patients.
We have studied the expression of CD1 antigens on peripheral blood mononuclear cells (PBMC) from acute hepatitis B patients in order to analyse a possible role for CD1 antigens in hepatitis B virus (HBV) infection. Using immunofluorescence and the monoclonal antibodies which recognized CD1a, CD1b and CD1c molecules, we have shown that CD1 antigens were expressed on PBMC from acute hepatitis B patients but not from other acute and chronic liver disease. Dot blot analysis on nitrocellulose sheets of the lysates of the cells confirmed these observations. Cell fractionation and double-labelling experiments clearly demonstrated the CD1 antigens were expressed only on non-T cells. Furthermore, CD1 antigens were coexpressed with hepatitis B surface antigen (HBsAg) on the surface of Ig-positive cells. These results could indicate that CD1 expression may be associated with the lymphotropic effect of HBV.
Roisman
F R
FR
Hospital de Clinicas José de San Martin, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
Salamone
M C
MC
Mota
A
A
Fainboim
H
H
Fainboim
L
L
D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal; D018949:Antigens, CD1; D000943:Antigens, Differentiation / Q000032:analysis*; D000954:Antigens, Surface / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D006509:Hepatitis B / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008297:Male
D000911:Antibodies, Monoclonal; D018949:Antigens, CD1; D000943:Antigens, Differentiation; D000954:Antigens, Surface