Detection of Rh D-associated epitopes in human and animal tissues using human monoclonal anti-D antibodies.
The distribution of Rh D-associated epitopes in human and animal tissues has been assessed by immunochemical techniques using six human monoclonal anti-D antibodies. The IgM antibody MAD-2 reacted predominantly with endothelial cells lining blood vessels and with animal leucocytes and human leucocytes from both Rh D-positive and Rh D-negative donors. Immunoblotting revealed reactivity with a 55 kDa tissue component which was most prominent in homogenates of heart, lung and spleen. In contrast, the IgG antibodies UCHD4 and FOG-1 stained smooth muscle, and UCHD4 in addition weakly stained cardiac and skeletal muscle and the glomeruli of kidney. Immunoblotting with UCHD4 revealed reactivity with a 27 kDa tissue component which was most prominent in heart homogenates. The results show that the six monoclonal anti-D antibodies recognize at least four different epitopes and that Rh D-associated epitopes may occur in non-erythroid cell types.
Thorpe
S J
SJ
National Institute for Biological Standards and Control, Potters Bar, Herts.
D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D000939:Epitopes / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D007518:Isoantibodies; D051379:Mice; D010455:Peptides / Q000276:immunology*; D011817:Rabbits; D051381:Rats; D012204:Rh-Hr Blood-Group System / Q000276:immunology*; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007518:Isoantibodies; D010455:Peptides; C033242:RH-antibodies; D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen