A simple method for preparing thin (10 microM) histological sections of undecalcified plastic embedded bone with implants.
A simple method for preparing undecalcified thin sections of bone with implants has been developed. After exposing a surface of bone and implant in a plastic block by sawing thick sections, the surface is stained prior to making a thin section. A glass coverslip is affixed with a thin layer of cement to the stained surface to stabilize the tissue and implant during sectioning. A mixture of glycerine and water is used as a coolant and lubricant. The orientation in situ is preserved allowing demonstration of bone architecture and cells, and the tissue-implant interface.
van der Lubbe
H B
HB
Department of Biomaterials Sciences, ACTA, Free University, Amsterdam, The Netherlands.
Klein
C P
CP
de Groot
K
K
D000818:Animals; D001672:Biocompatible Materials / Q000032:analysis*; D001842:Bone and Bones / Q000032:analysis*; D002516:Ceramics; D005898:Glass; D006652:Histological Techniques; D020366:Methylmethacrylate; D008768:Methylmethacrylates; D008867:Microtomy / Q000379:methods*; D011817:Rabbits; D013194:Staining and Labeling / Q000379:methods; D014025:Titanium
D001672:Biocompatible Materials; D008768:Methylmethacrylates; D020366:Methylmethacrylate; D014025:Titanium