Apparent molecular weight of the substance P binding site in rat brain.
Substance P (SP) binding sites in rat cerebral cortical membranes were specifically and covalently labelled by means of disuccinimidyl suberate (DSS), a bifunctional cross-linking reagent, to determine the apparent molecular weight of the SP binding site. Cross-linking of [3H]SP by 1 mM DSS to membranes revealed a specifically labelled single protein with an apparent molecular weight of 46,000, as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis and gel filtration analyses. This labelling was inhibited by non-radioactive SP and was not changed by the presence or absence of GTPrS suggesting that the label was bound to the binding site of SP receptors.
Nakata
Y
Y
Department of Pharmacology, Hiroshima University School of Medicine, Japan.
Hiraoka
C
C
Segawa
T
T
D000818:Animals; D001923:Brain Chemistry; D002850:Chromatography, Gel; D066298:In Vitro Techniques; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains; D018040:Receptors, Neurokinin-1; D017981:Receptors, Neurotransmitter / Q000032:analysis*; D013373:Substance P / Q000032:analysis*
D018040:Receptors, Neurokinin-1; D017981:Receptors, Neurotransmitter; D013373:Substance P