[A micromethod for determining lipoprotein cholesterol in capillary blood].
To measure the total cholesterol (CS) and high-density lipoprotein cholesterol (HDLP CS), the blood collected from the finger is mixed with an equal volume of physiologic saline with EDTA and the mixture is collected into a polyethylene tube to separate the diluted plasma from the formed elements by centrifugation. Low- and very low-density lipoprotein CS are sedimented after addition of heparin and Mn2+ ions to diluted plasma. Comparison of the values of the total CS and HDLP CS in the capillary blood plasma and in the blood sera of 20 patients has shown no significant difference between the two methods. The reproducibility and accuracy of the micro- and macromethods, compared in examinations of the same pool of sera, did not differ. This permits the use of the suggested micromethod for the aforesaid measurements during screenings of the population.
Finagin
L K
LK
Litovka
I G
IG
D000328:Adult; D002196:Capillaries; D002784:Cholesterol / Q000097:blood*; D008078:Cholesterol, LDL / Q000097:blood*; D015243:Cholesterol, VLDL / Q000097:blood*; D004492:Edetic Acid; D005385:Fingers / Q000098:blood supply; D006493:Heparin; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D008297:Male; D008345:Manganese; D008875:Middle Aged
D008078:Cholesterol, LDL; D015243:Cholesterol, VLDL; D008074:Lipoproteins; D008345:Manganese; D006493:Heparin; D002784:Cholesterol; D004492:Edetic Acid
Mikrometod opredeleniia kholesterina lipoproteidov v kapilliarnoĭ krovi.