Production of suppressor factor by T-cells from mice immunized with pneumococcal polysaccharide.
Factor(s) derived from plate purified T-cell cultures obtained from mice 16 hr after immunization with Type III pneumococcal polysaccharide (SSS-III), suppressed the antibody response to SSS-III. Such suppression was antigen specific. Adsorption of the suppressor factor (SF) with SSS-III covalently bound to Sepharose 4B column did not alter the ability of SF to suppress the antibody response. However, adsorption of SF with Ig+(B) cells taken from mice 48 hr after immunization with an immunogenic dose (0.5 ug) of SSS-III, completely removed the suppressive activity. Suppression of the antibody response was observed only when SF was administered 24 hr before to 24 hr after immunization with SSS-III. These results suggest that antigen specific suppressor T cells release soluble factor(s) which acts directly on antigen-stimulated B cells or may inhibit the induction of amplifier T-cells.
Taylor
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CE
Medical College of Pennsylvania, Department of Microbiology and Immunology, Philadelphia 19129.
Bright
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D000327:Adsorption; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D000939:Epitopes; D005260:Female; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D013491:Suppressor Factors, Immunologic / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000276:immunology*
D000939:Epitopes; D011135:Polysaccharides, Bacterial; D013491:Suppressor Factors, Immunologic; C026604:pneumococcal polysaccharide, type III