Changes in fast axonally transported proteins in the regenerating rabbit vagus nerve.
Regeneration was induced in rabbit vagus nerves by a crush injury. Fourteen and 50 days later [35S]methionine-radiolabelled, fast axonally transported proteins were separated by one- and two-dimensional electrophoresis. Quantitative densitometric analysis of fluorographs from one-dimensional separations showed increases in the radiolabelling of fast transported proteins of 45 and 20 kDa, and a decrease in the radiolabelling of a 26 kDa protein 14 days after crush injury, which were confirmed by two-dimensional separations. These increases were maintained at 50 days post-crush. The proteins have similar molecular weights and isoelectric points to two growth-associated proteins known to have important roles in growth and regeneration.
Archer
D R
DR
Department of Pharmacology and Therapeutics, University of Liverpool, U.K.
McLean
W G
WG
D000818:Animals; D001370:Axonal Transport; D008297:Male; D008715:Methionine; D008970:Molecular Weight; D009416:Nerve Regeneration; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011817:Rabbits; D014630:Vagus Nerve / Q000378:metabolism* / Q000502:physiology
D009419:Nerve Tissue Proteins; D008715:Methionine