Tissue protein synthesis and nucleic acid concentrations in steers treated with somatotropin.
The effect of injection with bovine somatotropin (bST) on the fractional rate of protein synthesis (FSR) in tissues of beef steers was studied using a continuous infusion of [1-14C]leucine. Minimum and maximum FSR were calculated from free leucine specific radioactivity (SRA) in plasma or tissue homogenate respectively. Tissue nucleic acid concentrations were also quantified. Tissue samples were obtained from several muscles, sections of the small intestine and liver. In response to bST, both minimum and maximum FSR increased in muscle but not liver or intestinal tissues. Absolute synthesis rate increased in several muscles and small intestine tissues. Treatment with bST increased the relative SRA of protein-bound leucine in muscles compared with liver; increased the amount of protein synthesis per unit empty body-weight (EBW) in most muscles; and increased weight of small intestine relative to EBW, suggesting a differential response between liver and the other tissues measured. Compositional changes in response to bST occurred only in muscles. DNA concentration increased while protein:DNA decreased in the gastrocnemius muscle and RNA:DNA increased in the longissimus dorsi. The maximum percentage contribution of tissue protein synthesis to whole-body protein synthesis was 12.6, 25.7 and 20.5, and 13.0, 29.4 and 25.8 for liver, muscle, and small intestine in placebo-treated and bST-injected steers respectively.
Eisemann
J H
JH
US Department of Agriculture, Agricultural Research Service, Clay Center, Nebraska 68933.
Hammond
A C
AC
Rumsey
T S
TS
D000818:Animals; D002417:Cattle; D004247:DNA / Q000096:biosynthesis*; D013006:Growth Hormone / Q000494:pharmacology*; D007421:Intestine, Small / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D009124:Muscle Proteins / Q000096:biosynthesis; D009132:Muscles / Q000378:metabolism; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*
D009124:Muscle Proteins; D012313:RNA; D013006:Growth Hormone; D004247:DNA; D007930:Leucine