Influence of retention time on degradation of pancreatic enzymes by human colonic bacteria grown in a 3-stage continuous culture system.
Hydrolytic enzymes were measured in gut contents from four sudden death victims. Pancreatic amylase and total protease activities decreased distally from the small bowel to the sigmoid/rectum region of the large intestine, showing that considerable breakdown or inactivation of the enzymes occurred during gut transit. To determine whether pancreatic enzymes were substrates for the gut microflora, mixed populations of bacteria were grown in a 3-stage continuous culture system on a medium that contained pancreatic extract as the sole nitrogen source. The multichamber system (MCS) was designed to reproduce in vitro, the low pH, high nutrient, fast growth conditions of the caecum and right colon and the neutral pH, low nutrient, slow growth conditions of the left colon. Results showed that pancreatic amylase was resistant to breakdown by intestinal bacteria compared with the peptide hydrolases in pancreatic secretions. Leucine aminopeptidase, trypsin and to a lesser degree, chymotrypsin, were easily degraded by gut bacteria, but pancreatic elastase was comparatively resistant to breakdown. Protein degradation in the MCS, as determined by enzyme activities, protein concentration and ammonia and phenol production, increased concomitantly with system retention time over the range 24-69 h. These results suggest that intestinal bacteria play an important role in the breakdown of hydrolytic enzymes secreted by the pancreas and that this process and protein fermentation in general, is likely to occur maximally in individuals with extended colonic retention times.
Macfarlane
G T
GT
Medical Research Council, Dunn Clinical Nutrition Centre, Cambridge, UK.
Cummings
J H
JH
Macfarlane
S
S
Gibson
G R
GR
D000681:Amylases / Q000378:metabolism*; D001419:Bacteria / Q000378:metabolism*; D002918:Chymotrypsin / Q000378:metabolism; D003106:Colon / Q000382:microbiology*; D006801:Humans; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D010179:Pancreas / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*; D013997:Time Factors; D014357:Trypsin / Q000378:metabolism
D000681:Amylases; D010447:Peptide Hydrolases; D007931:Leucyl Aminopeptidase; D002918:Chymotrypsin; D010196:Pancreatic Elastase; D014357:Trypsin