Clonal analysis of the T-cell response of mice to herpes simplex virus: correlation between lymphokine production in vitro and the induction of delayed-type hypersensitivity and antiviral activity in vivo.
The properties of two morphologically distinct L3T4+, Lyt2- "helper" T-cell clones specific for herpes simplex virus were investigated. Both of the clones produced IL-3 and interferon, but neither produced IL-2. Clone D6.6 produced macrophage agglutinating factor, a fibronectin-like lymphokine, and also a delayed hypersensitivity (DH) response when injected locally into syngeneic mice. Despite the presence of a DH producing clone and a non-DH producing clone, both were able to reduce the local virus titre to an equivalent degree. It is suggested that this protective activity is associated with the production of interferon-gamma. The significance of these results to mechanisms of protection against herpes simplex virus in vivo is discussed.
Seid
M
M
Department of Pathology, University of Cambridge, UK.
Leung
K N
KN
Pye
C
C
Phelan
J
J
Nash
A A
AA
Godfrey
H P
HP
D000818:Animals; D000956:Antigens, Viral; D002460:Cell Line; D002478:Cells, Cultured; D002999:Clone Cells / Q000276:immunology; D005260:Female; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D066298:In Vitro Techniques; D007372:Interferons / Q000096:biosynthesis*; D007377:Interleukin-3 / Q000096:biosynthesis; D008222:Lymphokines / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D018139:Simplexvirus / Q000276:immunology* / Q000302:isolation & purification; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D014762:Viral Interference
D000956:Antigens, Viral; D007377:Interleukin-3; D008222:Lymphokines; D007372:Interferons