Blocking of the beta-latrotoxin channels in bimolecular lipid membranes by antibodies.
Asymmetric ion channels are formed in a bimolecular lipid membrane by beta-latrotoxin (LT) introduced to one (cis) side of the membrane. LT-specific antibodies added to the opposite (trans) side of the membrane block the current through the LT channels when a negative potential is applied to the cis side, no blockade is observed at positive potentials. LT-specific antibodies do not block the channel current when added to the cis compartment after removal of LT. LT-unspecific immunoglobulins have no influence on LT channel conductance.
Kolomytkin
O V
OV
1 Institute of Biological Physics, Academy of Sciences USSR, Moscow.
Kasymov
S K
SK
Salikhov
S I
SI
D000906:Antibodies / Q000276:immunology; D000937:Antigen-Antibody Reactions; D001180:Arthropod Venoms / Q000378:metabolism*; D007473:Ion Channels / Q000276:immunology / Q000378:metabolism*; D008051:Lipid Bilayers / Q000276:immunology / Q000378:metabolism*; D008564:Membrane Potentials; D013111:Spider Venoms / Q000276:immunology / Q000378:metabolism*
D000906:Antibodies; D001180:Arthropod Venoms; D007473:Ion Channels; D008051:Lipid Bilayers; D013111:Spider Venoms; C056637:beta-latrotoxin