Localization of cutaneous antigens by the immunogold-silver staining technique.
Immunogold-silver staining (IGSS) was evaluated for the localization of cutaneous antigens in frozen and paraffin-embedded tissue, employing antibodies to a variety of intracellular, cell-surface and extracellular epitopes in an indirect immunogold reaction, followed by silver enhancement. The principal advantages of IGSS are the avoidance of toxic reagents and the production of a silver precipitate that is permanent, clearly visible and of sufficient contrast with the reaction products of alternative immunolocalizing techniques to be of value in double-labelling procedures. It seems to localize antigenic determinants well, but does not appear to be particularly sensitive, especially for the demonstration of antibodies to extracellular constituents. Limitations of IGSS include the propensity to non-specific staining, and the need to vary the duration of the development stage during silver enhancement.
Allen
M H
MH
Laboratory of Applied Dermatopathology, United Medical School of Guy's Hospital, London, U.K.
Tidman
M J
MJ
MacDonald
D M
DM
D000941:Antigens / Q000032:analysis*; D000954:Antigens, Surface / Q000276:immunology; D001485:Basement Membrane / Q000276:immunology; D005069:Evaluation Studies as Topic; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000276:immunology; D012867:Skin / Q000276:immunology*
D000941:Antigens; D000954:Antigens, Surface; D007633:Keratins