title: Investigation of the stereochemical course of DNA synthesis catalysed by human immunodeficiency virus type 1 reverse transcriptase.
abstract: The Sp-isomer of thymidine 5'-O-(1-thiotriphosphate) is used as substrate by HIV-1 reverse transcriptase. The absolute configuration of the internucleotide linkage in the oligonucleotide product was identified by 31p NMR spectroscopy to be the Rp-isomer, indicating that incorporation of dTTP alpha S into the oligonucleotide proceeded with inversion of configuration at the alpha-phosphorus. The mechanistic implications of these observations are discussed.
authors: Division of Virology, Wellcome Research Laboratories, Research Triangle Park, North Carolina 27709.
authors: S
authors: S
authors: Hopkins
authors: P A
authors: PA
authors: Furman
authors: G R
authors: GR
authors: Painter
mesh_terms: D002384:Catalysis; D004247:DNA / Q000096:biosynthesis* / Q000648:ultrastructure; D006678:HIV / Q000201:enzymology*; D066298:In Vitro Techniques; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D018894:Reverse Transcriptase Inhibitors; D013237:Stereoisomerism
chemical_list: D018894:Reverse Transcriptase Inhibitors; D004247:DNA; D012194:RNA-Directed DNA Polymerase