title: Mitochondrial rRNA-containing petite strains of yeast (Saccharomyces cerevisiae) show a normal nuclear-mitochondrial stringent response.
abstract: The nuclear-mitochondrial stringent response was examined in isonuclear rho+, 21S rRNA-containing rho-, and rho o strains of S. cerevisiae. By 30 min after nutritional downshift, nuclear rDNA transcription falls to 15% of control levels congruently in all strains, as assayed via whole-cell RNA or by hybrid selection of specific double-labeled transcripts. Both in vivo and in vitro, the mitochondrial stringent response is identical between the rho- strain and its parental rho+ strain, and in both, the kinetics and magnitude of the organellar response mirror those of the nuclear response. The data show that mitochondrial transcription and protein synthesis are not required for stringent regulation of either nuclear or mitochondrial rDNA transcription.
authors: Dept. Microbiology and Immunology, Medical College of Pennsylvania, Philadelphia.
authors: C M
authors: CM
authors: McEntee
authors: R
authors: R
authors: Cantwell
authors: L C
authors: LC
authors: Thomas
authors: A P
authors: AP
authors: Hudson
mesh_terms: D008928:Mitochondria / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*; D012331:RNA, Fungal / Q000235:genetics; D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D012331:RNA, Fungal; D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal; D012313:RNA