Tissue-specific in vitro transcription from the mouse myelin basic protein promoter.
The mouse myelin basic protein promoter was transcribed in brain nuclear extracts. The distal promoter region from -253 to -54 directed preferential transcription in brain extracts, whereas the same region repressed transcription activity in liver extracts. Stimulation of transcription was observed when the distal region was located only in a native orientation. The proximal region downstream from -53 alone still directed preferential transcription. It is suggested that cooperative function by the two promoter regions may be required for higher specificity.
Tamura
T
T
Division of Behavior and Neurobiology, National Institute for Basic Biology, Okazaki, Japan.
Aoyama
A
A
Inoue
T
T
Miura
M
M
Okano
H
H
Mikoshiba
K
K
D000818:Animals; D001921:Brain / Q000378:metabolism*; D015342:DNA Probes; D004270:DNA, Circular / Q000235:genetics; D066298:In Vitro Techniques; D051379:Mice; D004676:Myelin Basic Protein / Q000096:biosynthesis / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D014158:Transcription, Genetic
D015342:DNA Probes; D004270:DNA, Circular; D004676:Myelin Basic Protein; D012333:RNA, Messenger