Anion channels contribute to the regulation of intracellular pH in human platelets.
The presence of extracellular bicarbonate potentiated platelet intracellular pH rises induced by thrombin. The effect was most remarkable in sodium-depleted buffers. This effect of bicarbonate was dose-dependent and was inhibited by anion channel blockers. Bicarbonate also potentiated the pHi recovery after acid loading, but had no effect on alkali loading. It was suggested that anion exchange, most probably that of bicarbonate/chloride, contributes to the regulation of intracellular pH in human platelets.
Ozaki
Y
Y
Central Clinical Laboratory, Yamanashi Medical College, Japan.
Yatomi
Y
Y
Kariya
T
T
Kume
S
S
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D000838:Anions; D001639:Bicarbonates / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism; D004357:Drug Synergism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007424:Intracellular Fluid / Q000378:metabolism; D007473:Ion Channels / Q000378:metabolism*; D007474:Ion Exchange; D017923:Sodium-Hydrogen Exchangers; D013917:Thrombin / Q000494:pharmacology
D000838:Anions; D001639:Bicarbonates; D002352:Carrier Proteins; D002712:Chlorides; D007473:Ion Channels; D017923:Sodium-Hydrogen Exchangers; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D013917:Thrombin; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid