Differences in hydrophobic properties for human alpha 2-macroglobulin and pregnancy zone protein as studied by affinity phase partitioning.
Human alpha 2-macroglobulin and pregnancy zone protein are related with regard to primary structure, physicochemical properties, and quarternary structure. Both proteins undergo conformational changes when they form complexes with proteinases or react with primary amines. The surface properties of the native, chymotrypsin-treated and methylamine-treated forms of alpha 2-macroglobulin and pregnancy zone protein were studied by partitioning in aqueous two-phase systems composed of 7.5% dextran T70 and 5% poly(ethylene glycol) 8000. All proteins and their derivatives had a high potential for hydrophobic interaction as analyzed in terms of affinity for poly(ethylene glycol) esters of fatty acids included in the phase systems. Treatment of alpha 2-macroglobulin with methylamine or chymotrypsin increased the surface hydrophobicity significantly compared to that of the native protein. No difference in hydrophobic interaction was found for native and methylamine-treated pregnancy zone protein, but the chymotrypsin-treated protein showed a marked increase in binding to the hydrophobic ligand. The changes in surface hydrophobicity parallel changes in receptor binding properties of the derivatized forms of alpha 2-macroglobulin and could be a signal for binding to cell-surface receptors, followed by internalization.
Birkenmeier
G
G
Institute of Biochemistry, Karl-Marx-University, Leipzig.
Carlsson-Bostedt
L
L
Shanbhag
V
V
Kriegel
T
T
Kopperschläger
G
G
Sottrup-Jensen
L
L
Stigbrand
T
T
D002498:Centrifugation; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002918:Chymotrypsin / Q000494:pharmacology; D003911:Dextrans; D006801:Humans; D008744:Methylamines / Q000494:pharmacology; D019308:Palmitic Acid; D010169:Palmitic Acids; D011092:Polyethylene Glycols; D011257:Pregnancy Proteins; D013499:Surface Properties; D000511:alpha-Macroglobulins
D003911:Dextrans; D008744:Methylamines; D010169:Palmitic Acids; D011257:Pregnancy Proteins; D000511:alpha-Macroglobulins; D019308:Palmitic Acid; D011092:Polyethylene Glycols; C027451:methylamine; D002918:Chymotrypsin