Caffeine interaction with the Ca-release channels of heavy sarcoplasmic reticulum. Evidence that 170 kD Ca-binding protein is a caffeine receptor of the Ca-channels.
The study of Ca2+- and caffeine-induced Ca2+ release from heavy sarcoplasmic reticulum vesicles under the different conditions suggests that Ca2+ and caffeine can interact with the common receptor of the Ca-release channels. The reticulum membranes were solubilized using nonionic detergent polyoxyethylene 9-lauryl ether, and affinity chromatography on reactive red 120-agarose was carried out. The 170 kD Ca-binding protein which is eluted by caffeine is the most probable candidate for the caffeine receptor of the Ca-channels.
Rubtsov
A M
AM
Department of Biochemistry, Moscow State University, USSR.
Murphy
A J
AJ
D000818:Animals; D017317:Annexin A6; D002110:Caffeine / Q000378:metabolism* / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002135:Calcium-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008634:Mersalyl / Q000494:pharmacology; D008970:Molecular Weight; D009132:Muscles / Q000378:metabolism; D011817:Rabbits; D011983:Receptors, Purinergic / Q000302:isolation & purification / Q000378:metabolism*; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*
D017317:Annexin A6; D002135:Calcium-Binding Proteins; D007473:Ion Channels; D011983:Receptors, Purinergic; D002110:Caffeine; D008634:Mersalyl; D002118:Calcium