Preparation of nanoparticles conjugated with enzyme and antibody and their use in heterogeneous enzyme immunoassays.
Enzyme conjugates suitable for enzyme-linked immunosorbent assays can be conveniently prepared by binding enzyme and antibody to nanoparticles. Peroxidase and antitransferrin or anti-alpha-fetoprotein were co-immobilized to tresyl activated 40 nm silica particles and the particle conjugates used in heterogeneous enzyme immunoassays for the evaluation of transferrin and alpha-fetoprotein within the concentration range 0.25-1000 ng/ml.
Nilsson
K G
KG
Carbohydrates International, Arlöv, Sweden.
D006801:Humans; D007124:Immunoenzyme Techniques; D014168:Transferrin / Q000032:analysis; D000509:alpha-Fetoproteins / Q000032:analysis
D014168:Transferrin; D000509:alpha-Fetoproteins