Kinetic properties of poly (2'-O-methylcytidylic acid)-directed reverse transcriptase reaction.
Poly (2'-O-methylcytidylic acid) is recognized as a template in reactions catalyzed by RNA-dependent DNA polymerases in the presence of Mn2+ as divalent cation. We report that kinetic data obtained for dGTP and template under optimal experimental conditions in the reaction catalyzed by reverse transcriptase showed some similarities between the poly (2'-O-methylcytidylic acid)/Mn2+ and polyribocytidylic acid/Mg2+ systems. The reaction was inhibited by the action of N-ethylmaleimide and novobiocin, and to a lesser extent by ethidium bromide and tetramethyl ethidium bromide.
Aoyama
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Departamento de Bioquímica, Instituto de Biologia, UNICAMP, Campinas, SP, Brasil.
D001354:Avian Leukosis Virus / Q000201:enzymology*; D009189:Avian Myeloblastosis Virus / Q000201:enzymology*; D001665:Binding Sites; D006160:Guanosine Triphosphate / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D011066:Poly C / Q000493:pharmacokinetics*; D011131:Polyribonucleotides / Q000493:pharmacokinetics*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*
D011131:Polyribonucleotides; D011066:Poly C; D008345:Manganese; D006160:Guanosine Triphosphate; D012194:RNA-Directed DNA Polymerase; D008274:Magnesium