Competition binding assay in cell culture for identification of epitopes on enveloped and naked viruses.
Virus infected monolayers, in wells of 96-well plates, could be used as antigen in competition binding assays to identify epitopes on respectively Semliki Forest virus, encephalomyocarditis virus and mumps virus.
Oosterlaken
T A
TA
Laboratory of Microbiology, State University of Utrecht, The Netherlands.
Vlaspolder
F
F
Fransen
R
R
Harmsen
T
T
Kraaijeveld
C A
CA
Snippe
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology / Q000378:metabolism*; D001667:Binding, Competitive; D004686:Encephalomyocarditis virus / Q000276:immunology* / Q000502:physiology; D000939:Epitopes / Q000276:immunology / Q000378:metabolism*; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques; D007739:L Cells; D051379:Mice; D009109:Mumps virus / Q000276:immunology* / Q000502:physiology; D012672:Semliki forest virus / Q000276:immunology* / Q000502:physiology; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes