Encapsulation of the UV-DNA repair enzyme T4 endonuclease V in liposomes and delivery to human cells.
T4 endonuclease V, a pyrimidine-dimer-specific DNA repair enzyme, was encapsulated in liposomes under mild conditions. The encapsulated enzyme was active, and when applied to ultraviolet (UV)-irradiated human cells in culture, the liposomes increased incision of UV-irradiated cellular DNA, enhanced DNA repair replication, and enhanced survival of UV-irradiated cells. This method is a first step in a new approach for topical application of DNA repair enzymes to human skin to prevent skin cancer.
Ceccoli
J
J
Applied Genetics Inc., Freeport, New York 11520.
Rosales
N
N
Tsimis
J
J
Yarosh
D B
DB
D002461:Cell Line, Transformed; D002470:Cell Survival; D002478:Cells, Cultured; D004247:DNA / Q000187:drug effects / Q000528:radiation effects*; D004249:DNA Damage; D004260:DNA Repair; D004337:Drug Carriers; D004706:Endodeoxyribonucleases / Q000008:administration & dosage* / Q000494:pharmacology; D000078404:Epidermal Cells; D004817:Epidermis / Q000187:drug effects / Q000528:radiation effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D007633:Keratins; D008081:Liposomes; D014466:Ultraviolet Rays
D004337:Drug Carriers; D008081:Liposomes; D007633:Keratins; D004247:DNA; D004706:Endodeoxyribonucleases; C026044:deoxyribonuclease V