[ATP synthesis in Staphylococcus aureus cells during induction of membrane potentials and proton gradient].
The dynamics of ATP synthesis in Staphylococcus aureus cells was studied during membrane potential induction and K+ gradient generation in the presence of valinomycin. The starting level of intracellular ATP was 0.05 mM. Valinomycin (30 micrograms/ml) caused an increment of the intracellular ATP level up to 0.25 mM. The protonophore uncoupler, m-chlorinecarbonylcyanidephenylhydrazonium, and the H+-ATPase inhibitor, N,N'-dicyclohexylcarbodiimide, effectively suppress ATP synthesis induced by valinomycin. No ATP synthesis occurs at K+ concentration of 200 mM. The transmembrane gradient formation results in the synthesis of a smaller amount of ATP (0.10 mM).
Vinnikov
A I
AI
D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism; D008564:Membrane Potentials; D011188:Potassium / Q000378:metabolism; D013211:Staphylococcus aureus / Q000378:metabolism*; D014634:Valinomycin / Q000494:pharmacology
D007473:Ion Channels; D014634:Valinomycin; D000255:Adenosine Triphosphate; D011188:Potassium
Sintez ATP v kletkakh Staphylococcus aureus pri navedenii membrannogo potentsiala i gradienta protonov.