Sequence-specific isotope effects on the cleavage of DNA by bleomycin.
Bleomycin is a metal- and oxygen-dependent DNA cleaver. The chemistry of DNA damage has been proposed to involve rate-limiting abstraction of the 4'-hydrogen. A DNA fragment has been prepared that contains [4'-2H]thymidine residues of high isotopic content. Primary kinetic isotope effects have been directly observed at individual thymidine residues with DNA sequencing technology.
Kozarich
J W
JW
Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742.
Worth
L
L
Frank
B L
BL
Christner
D F
DF
Vanderwall
D E
DE
Stubbe
J
J
D001483:Base Sequence; D001761:Bleomycin; D004249:DNA Damage; D003903:Deuterium; D007501:Iron; D010100:Oxygen; D013329:Structure-Activity Relationship; D013936:Thymidine
D001761:Bleomycin; D003903:Deuterium; D007501:Iron; D010100:Oxygen; D013936:Thymidine