title: Repair-modification and evolution of the eukaryotic genome organization.
abstract: For a complete reconstruction of the damaged unmethylated islands, in theory, the conventional excision-repair is sufficient. For a complete reconstruction of the damaged methylated domains, a coupling has to take place involving the excision-repair (able to reestablish their ATGC-language) plus the DNA-methylase (able to reestablish their modified ATGC5mC-language). This coupling, defined as "repair-modification," is essentially functioning during the S-phase, because the DNA-polymerase beta (pol beta) is active during the whole cell cycle, whereas the DNA-methylase (met) is active in S and appears to be repressed or inactive during the major part of G1 and during the phases G2 and M. Consequently, after damage, some silent genes might become expressed during these phases, if it is true that DNA methylation is inversely proportional to transcription. Repair-modification should, therefore, exert a continuous differential pressure on evolution of given parts of the genome, when they are methylated to a different extent. According to Darwinian concepts, repair-modification would lead to a high variability, especially of uncoding DNA sequences (if hypermethylated), whereas on the basis of this variability, selection might favor transposition of specific regulatory elements into given transcriptional units. In these, the conservative nature of the coding elements (if unmethylated) would obviously be ensured by the conventional excision-repair.
authors: Institute of Experimental Medicine, CNR, Rome, Italy.
authors: P
authors: P
authors: Volpe
authors: T
authors: T
authors: Eremenko
mesh_terms: D000818:Animals; D001482:Base Composition; D005075:Biological Evolution; D002453:Cell Cycle; D002499:Centrifugation, Density Gradient; D002851:Chromatography, High Pressure Liquid; D015254:DNA Modification Methylases / Q000378:metabolism*; D004256:DNA Polymerase I / Q000378:metabolism; D004260:DNA Repair; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007399:Interphase; D008745:Methylation; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D015254:DNA Modification Methylases; D004256:DNA Polymerase I; D004259:DNA-Directed DNA Polymerase