Pharmacological characterization of muscarinic receptors mediating inhibition of adenylate cyclase activity in the rat retina.
Incubation of the rat retina with acetylcholine resulted in about a 20 to 30% decrease of basal cyclic AMP accumulation. Oxotremorine, arecoline, [4-hydroxy-2-butynyl]trimethylammonium chloride, m-chlorocarbanilate and carbachol also inhibited cyclic AMP accumulation. Nicotine had no effect. The response was blocked by atropine and pirenzepine but not gallamine. Intraocular injection of pertussis toxin 72 hr before testing also blocked the response to acetylcholine. The presence of forskolin exaggerated the response to acetylcholine. Intraocular injection of the cholinotoxin AF64A resulted in apparent supersensitivity of the response to acetylcholine. Our results suggest that rat retina contains muscarinic M1 receptors that are coupled negatively to adenylate cyclase.
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Department of Pharmacology, Ohio State University College of Medicine, Columbus.
Fertel
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Neff
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Hadjiconstantinou
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D008455:(4-(m-Chlorophenylcarbamoyloxy)-2-butynyl)trimethylammonium Chloride / Q000494:pharmacology; D000109:Acetylcholine / Q000494:pharmacology; D000067956:Adenylyl Cyclase Inhibitors; D000818:Animals; D001115:Arecoline / Q000494:pharmacology; D001388:Aziridines / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D002794:Choline / Q000031:analogs & derivatives / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D008297:Male; D010095:Oxotremorine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D011976:Receptors, Muscarinic / Q000378:metabolism*; D012160:Retina / Q000187:drug effects / Q000201:enzymology*
D000067956:Adenylyl Cyclase Inhibitors; D001388:Aziridines; D011976:Receptors, Muscarinic; D005576:Colforsin; D001115:Arecoline; D008455:(4-(m-Chlorophenylcarbamoyloxy)-2-butynyl)trimethylammonium Chloride; D010095:Oxotremorine; D002217:Carbachol; C044894:ethylcholine aziridinium; D000242:Cyclic AMP; D002794:Choline; D000109:Acetylcholine