A global response induced in Escherichia coli by redox-cycling agents overlaps with that induced by peroxide stress.
Escherichia coli treated with nontoxic levels of the superoxide-generating redox-cycling agents menadione and paraquat showed dramatic changes in protein composition as monitored by two-dimensional gel analysis. The distribution of proteins synthesized after treatment with these agents overlapped significantly with that seen after hydrogen peroxide treatment, and it included all the proteins in the oxyR regulon. The redox-cycling agents also elicited the synthesis of at least 33 other proteins that were not seen with hydrogen peroxide, including three heat shock proteins, the Mn-containing superoxide dismutase, the DNA repair protein endonuclease IV, and glucose-6-phosphate dehydrogenase. At least some of these redox-inducible proteins appear to be part of a specific response to intracellular superoxide. E. coli is thus equipped with a network of inducible responses against oxidative damage, controlled in multiple regulatory pathways.
Greenberg
J T
JT
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.
Demple
B
B
D001426:Bacterial Proteins / Q000096:biosynthesis; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D043223:Deoxyribonuclease IV (Phage T4-Induced); D004352:Drug Resistance, Microbial; D004706:Endodeoxyribonucleases / Q000096:biosynthesis; D004790:Enzyme Induction; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010269:Paraquat / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000096:biosynthesis; D014812:Vitamin K / Q000494:pharmacology*
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D014812:Vitamin K; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; D004706:Endodeoxyribonucleases; D043223:Deoxyribonuclease IV (Phage T4-Induced); C468972:endonuclease IV, E coli; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D010269:Paraquat