Selective silver-staining methods for RNA and proteins in the same polyacrylamide gels.
Two silver-staining methods for selective and ultrasensitive detection of RNAs and proteins in the same polyacrylamide gels were developed, both derived from procedures recommended for protein staining. The first, a double-staining technic with Coomassie brilliant blue and ammoniacal silver, allows visualization of RNAs as negative bands and proteins as dark brown bands. The second is also a double-staining technique, but uses silver in both steps. This second method develops the RNA bands first and then the protein bands. These techniques, especially the second, permit characterization of the different components of ribonucleoproteic complexes in the same electrophoresis gels.
Paleologue
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Laboratoire de Biochimie Médicale, Université Claude Bernard, Lyon I, Villeurbanne, France.
Reboud
J P
JP
Reboud
A M
AM
D004591:Electrophoresis, Polyacrylamide Gel; D011506:Proteins / Q000032:analysis*; D012313:RNA / Q000032:analysis*; D012834:Silver; D013194:Staining and Labeling
D011506:Proteins; D012834:Silver; D012313:RNA