title: Immunological evidence for differences in the exposed regions of OmpF porins from Escherichia coli B and K-12.
abstract: Nine monoclonal antibodies (MoF 0-8) directed against the native form (trimeric) of outer membrane protein OmpF of Escherichia coli B were obtained and characterized. All these antibodies bind to OmpF porin in intact E. coli B cells but not OmpF from E. coli K-12 cells which only differ at positions 66, 117 and 262 in the sequence. These antibodies exhibit a specificity to the native form, failing to recognize the denatured form in a liquid immunorecognition assay. Four tested antibodies are able to protect against colicin A, a bacteriotoxin using OmpF as receptor. One monoclonal antibody (MoF 0) is specific to the external topology of native porin in the outer membrane and three antibodies could recognize epitopes present in each conformation of subunits of trimer form. It is concluded that the region around the 66th and more probably around the 262nd amino acids are involved in cell-surface exposed epitopes. Moreover, these results support the assumption that the conformation of protruding regions of OmpF from E. coli B and K-12 are different.
authors: Centre de Biochimie et de Biologie Moléculaire, CNRS, Marseille, France.
authors: J M
authors: JM
authors: Pagès
authors: C
authors: C
authors: Pagès
authors: A
authors: A
authors: Bernadac
authors: P
authors: P
authors: Prince
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001667:Binding, Competitive; D002462:Cell Membrane / Q000276:immunology; D003087:Colicins / Q000378:metabolism; D000939:Epitopes / Q000032:analysis*; D004926:Escherichia coli / Q000145:classification / Q000276:immunology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D018272:Porins
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D003087:Colicins; D000939:Epitopes; D018272:Porins