title: Distribution model for the intact urokinase and urokinases modified by soluble macromolecules in rat and mouse bodies.
abstract: The activity of intact urokinase (UK) and urokinases modified by soluble macromolecules (dextran and dextran sulfate sodium) in a mouse body was traced after injection of the 125I-labelled enzymes. The residual fraction of the enzyme in blood can be correlated with the time (t) as follows; Xb = Ae-at + Be-bt + Ce-ct Since a greater than b greater than c, the residual fraction of the enzyme in blood chiefly depends on the magnitude of parameter C. The constant C for modified urokinase was larger than that for urokinase, showing the relative residual in blood was increased by modification of the enzyme. The apparent utilization in 50 min was 13.8% for intact UK, 28.1% for the dextran-UK and 25.2% for the sulfate dextran-UK. Therefore, the apparent utilization of UK in blood was approximately doubled by the modification. Since the half-lives of UK and modified UK in kidney and liver were not long, there were no unacceptable accumulation of the enzymes.
authors: Dalian College of Light Industry, China.
authors: F X
authors: FX
authors: Jin
authors: Y H
authors: YH
authors: Cheng
authors: K
authors: K
authors: Toda
mesh_terms: D000818:Animals; D003911:Dextrans / Q000494:pharmacology; D051379:Mice; D008954:Models, Biological; D051381:Rats; D013927:Thrombosis / Q000188:drug therapy; D014018:Tissue Distribution; D014568:Urokinase-Type Plasminogen Activator / Q000493:pharmacokinetics*
chemical_list: D003911:Dextrans; D014568:Urokinase-Type Plasminogen Activator