title: Extensive changes in DNA methylation patterns accompany activation of a silent T-DNA ipt gene in Agrobacterium tumefaciens-transformed plant cells.
abstract: We crossed a male-sterile, Agrobacterium-transformed Nicotiana tabacum plant that contains a silent, hypermethylated T-DNA ipt oncogene with a normal tobacco plant and found that the methylated state of the ipt gene was stably inherited through meiosis in the offspring. However, when tissues of these plants were placed in cell culture, the ipt gene was spontaneously reactivated in a very small fraction of the cells; if 5-azacytidine was added to the culture medium, ipt gene reactivation occurred at high frequency. We analyzed the pattern of DNA methylation in a region spanning the ipt gene in a line that does not express the ipt gene, in five derivatives of this line that reexpressed the ipt gene either spontaneously or after 5-azacytidine treatment, and in tissues of a sibling of this line that reexpressed ipt spontaneously. We found that the ipt locus was highly methylated in the unexpressed state but that spontaneous or 5-azacytidine-induced reexpression always resulted in extensive demethylation of a region including 5' upstream, coding, and 3' downstream regions of the ipt gene. The role of DNA methylation in gene regulation in this system is discussed.
authors: Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison 53706-0569.
authors: M C
authors: MC
authors: John
authors: R M
authors: RM
authors: Amasino
mesh_terms: D019883:Alkyl and Aryl Transferases; D001374:Azacitidine / Q000494:pharmacology; D002461:Cell Line, Transformed; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000378:metabolism; D015870:Gene Expression / Q000502:physiology; D008745:Methylation; D010641:Phenotype; D010941:Plant Tumors; D010947:Plants, Toxic; D010957:Plasmids / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D012231:Rhizobium / Q000235:genetics*; D014026:Nicotiana; D014158:Transcription, Genetic / Q000502:physiology; D014166:Transferases / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D012333:RNA, Messenger; D014166:Transferases; D019883:Alkyl and Aryl Transferases; C024274:adenylate isopentenyltransferase; D001374:Azacitidine