title: Evaluation of staining methods for identifying Campylobacter pylori.
abstract: Campylobacter pylori has been implicated in the pathogenesis of peptide ulcer disease. The rapid identification of this organism may depend upon histologic diagnosis, because culture methods are complex and require a minimum of seven days in order to identify a negative specimen. The purpose of this study was to determine which stain used to identify this organism was the most cost-effective and easiest to perform and interpret on a routine basis. Sixty-one consecutive gastric antral biopsies were stained with hematoxylin and eosin, Giemsa, Brown-Brenn, and Warthin-Starry, with 23 of the cases stained by Brown-Hopps. Of the stains tested, the Wright-Giemsa was the easiest to perform. The organisms on the Wright-Giemsa showed a smooth, uniform purple color, whereas the Warthin-Starry gave the organism a granular appearance that at times could be confused for silver precipitate. Both the Wright-Giemsa and Brown-Hopps stain had the highest degree of identification of the organism (defined by percent positivity). The routine use of the Wright-Giemsa stain for identification of C. pylori in antral biopsies is recommended.
authors: Marshall University School of Medicine, Department of Pathology, Huntington, West Virginia 25702.
authors: E
authors: E
authors: Madan
authors: J
authors: J
authors: Kemp
authors: T U
authors: TU
authors: Westblom
authors: M
authors: M
authors: Subik
authors: S
authors: S
authors: Sexton
authors: J
authors: J
authors: Cook
mesh_terms: D000208:Acute Disease; D002167:Campylobacter / Q000032:analysis*; D002169:Campylobacter Infections / Q000382:microbiology / Q000473:pathology; D002908:Chronic Disease; D004801:Eosine Yellowish-(YS); D005756:Gastritis / Q000382:microbiology / Q000473:pathology; D006416:Hematoxylin; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology / Q000473:pathology; D008297:Male; D011706:Pyloric Antrum / Q000032:analysis; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D004801:Eosine Yellowish-(YS); D006416:Hematoxylin