The nucleotide sequence of POX18, a gene encoding a small oleate-inducible peroxisomal protein from Candida tropicalis.
We report the molecular cloning and nucleotide sequence of the nuclear gene, POX18, encoding an oleate-inducible peroxisomal protein from the yeast Candida tropicalis. POX18 has a single open reading frame of 381 nucleotides (nt), which encodes a protein of 127 amino acids. The predicted Mr of this protein is 13,792. Codon usage in the expression of POX18 is non-random, and shows a pattern similar to that used for other peroxisomal genes from C. tropicalis and highly expressed genes from Saccharomyces cerevisiae. Northern analysis of total RNA from oleate-grown cells determined that POX18 mRNA is approximately 750 nt in length. The POX18 gene was expressed in vitro, which resulted in a single translation product that co-migrated in denaturing polyacrylamide gels with an abundant peroxisomal protein (apparent mass of 16 kDa) and was immunoprecipitated by an antiserum against peroxisomal protein.
Szabo
L J
LJ
Rockefeller University, New York, NY 10021.
Small
G M
GM
Lazarow
P B
PB
D000595:Amino Acid Sequence; D001483:Base Sequence; D002175:Candida / Q000235:genetics*; D003001:Cloning, Molecular; D003062:Codon; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D015151:Immunoblotting; D008830:Microbodies; D008969:Molecular Sequence Data; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology; D010957:Plasmids; D011506:Proteins / Q000235:genetics*; D012313:RNA / Q000032:analysis / Q000235:genetics; D014158:Transcription, Genetic
D003062:Codon; D009829:Oleic Acids; D011506:Proteins; D019301:Oleic Acid; D012313:RNA; D004247:DNA