Reversible inactivation by dextran sulfate of bovine thrombin in dilute solution.
Dextran sulfate (DS)-enhanced inactivation of alpha-thrombin was faster at pH 6 than at pH 8 in the absence of NaCl. Substrate protected only partly thrombin from inactivation by DS at pH 6. Some amidolytic activity of alpha-thrombin inactivated by DS was restored time-dependently by the addition of NaCl. Moreover, beta and gamma-thrombin, and other intermediates were not formed from alpha-thrombin with DS-enhanced inactivation. All these results indicate that autolysis of alpha-thrombin in dilute solution is not responsible for enhancement of reversible inactivation of alpha-thrombin by DS.
Oshima
G
G
School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
D000818:Animals; D002417:Cattle; D016264:Dextran Sulfate; D003911:Dextrans / Q000037:antagonists & inhibitors*; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D008934:Mitogens / Q000037:antagonists & inhibitors*; D013917:Thrombin
D003911:Dextrans; D008934:Mitogens; D016264:Dextran Sulfate; D013917:Thrombin