Heterogeneity of tachykinin-like immunoreactive peptides in rat spinal cord and dorsal root ganglia.
The concentrations of tachykinins in rat spinal cord and dorsal root ganglia (DRGs) were measured using a combination of high performance liquid chromatography (HPLC) and radioimmunoassays (RIAs). Substance P-like immunoreactivity (SPLI) was found to be significantly higher than either substance K-like immunoreactivity (SKLI) or neuromedin K-like immunoreactivity (NMKLI) in both tissues. In the spinal cord, the concentration of SKLI was comparable to that of NMKLI. In DRGs, NMKLI is present at concentrations much lower than those of SKLI or SPLI. In addition to immunoreactive components co-eluting with the three mammalian tachykinins SP, SK and NMK, analyses using reverse-phase HPLC revealed an immunoreactive peak co-eluting with the C-terminal octapeptide of SK (SK3-10), and a yet to be identified peak eluting before SK. This study also demonstrates the use of a novel and highly specific RIA for NMK to measure NMKLI without the need of reverse-phase HPLC.
Too
H P
HP
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115.
Cordova
J L
JL
Maggio
J E
JE
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003429:Cross Reactions; D005260:Female; D005727:Ganglia, Spinal / Q000032:analysis*; D008297:Male; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000032:analysis*; D013373:Substance P / Q000302:isolation & purification; D015320:Tachykinins / Q000276:immunology / Q000302:isolation & purification*
D015320:Tachykinins; D013373:Substance P