Antigenic probes locate binding sites for the glycolytic enzymes glyceraldehyde-3-phosphate dehydrogenase, aldolase and phosphofructokinase on the actin monomer in microfilaments.
The topology of the interfaces between actin monomers in microfilaments and three glycolytic enzymes (glyceraldehyde-3-phosphate dehydrogenase, aldolase and phosphofructokinase) was investigated using several specific antibodies directed against precisely located sequences in actin. A major contact area for glyceraldehyde-3-phosphate dehydrogenase was characterized in a region near residue 103. This interaction altered, by long-range conformational changes, the reactivity of antigenic epitopes in the C-terminal part of actin. The interface between actin and aldolase appeared to involve a sequence around residue 299 in the C-terminal region of actin. The interaction of phosphofructokinase, in contrast, modified the reactivity of all antibodies tested. Finally, the phosphagen kinases arginine kinase and creatine kinase showed no interaction with the microfilament.
Méjean
C
C
UPR 8402 Centre de Recherches de Biochimie Macromoléculaire (CNRS), U249 INSERM, Université de Montpellier I, France.
Pons
F
F
Benyamin
Y
Y
Roustan
C
C
D008841:Actin Cytoskeleton / Q000378:metabolism*; D000199:Actins / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000936:Antigen-Antibody Complex; D001665:Binding Sites; D002846:Chromatography, Affinity; D003599:Cytoskeleton / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D005634:Fructose-Bisphosphate Aldolase / Q000276:immunology / Q000378:metabolism*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000276:immunology / Q000378:metabolism*; D006019:Glycolysis; D007700:Kinetics; D009132:Muscles / Q000201:enzymology; D010732:Phosphofructokinase-1 / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits
D000199:Actins; D000906:Antibodies; D000936:Antigen-Antibody Complex; D000939:Epitopes; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D010732:Phosphofructokinase-1; D005634:Fructose-Bisphosphate Aldolase