Identification of amino acids recognized by syncytium-inhibiting and neutralizing monoclonal antibodies to the human parainfluenza type 3 virus fusion protein.
Neutralizing monoclonal antibodies specific for the fusion (F) glycoprotein of human parainfluenza type 3 virus (PIV3) were used to select neutralization-resistant antigenic variants. Sequence analysis of the F genes of the variants indicated that their resistance to antibody binding, antibody-mediated neutralization or to both was a result of specific amino acid substitutions within the neutralization epitopes of the F1 and F2 subunits. Comparison of the locations of PIV3 neutralization epitopes with those of Newcastle disease and Sendai viruses indicated that the antigenic organization of the fusion proteins of paramyxoviruses is similar. Furthermore, some of the PIV3 epitopes recognized by syncytium-inhibiting monoclonal antibodies are located in an F1 cysteine cluster region which corresponds to an area of the measles virus F protein involved in fusion activity.
Coelingh
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KV
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
Tierney
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EL
D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D001665:Binding Sites; D000939:Epitopes / Q000032:analysis; D009500:Neutralization Tests; D010224:Parainfluenza Virus 3, Human / Q000032:analysis*; D018108:Respirovirus / Q000032:analysis*; D014760:Viral Fusion Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D000939:Epitopes; D014760:Viral Fusion Proteins