title: Extragonadal effects of luteinizing hormone in mice.
abstract: LH is composed of isoforms which exhibit microheterogeneity. We recently demonstrated that a particular ovine or porcine LH preparation (G100-fr.3) stimulates kidney growth. This study was conducted to clarify the physiological role of this renotropic activity and other extragonadal effects of the ovine LH preparation in CD-1 mice. Hypophysectomy caused a significantly greater reduction in relative dry kidney weight (i.e. g/100 g body weight) when compared to adrenalectomy, castration, thyroidectomy, and castration plus thyroidectomy. Supplementation with G100-fr.3 in these animals partially restored not only kidney size but also DNA, RNA and protein content. Treatment with standard LH preparations (NIDDKoLH24 and G3-268DA), as well as PRL, GH, FSH and TSH, failed to reverse the renal atrophy induced by hypophysectomy and castration. Administration of testosterone to castrated hypophysectomized mice increased kidney weight and RNA content, but not renal DNA. The relative dry kidney weight increased significantly at the onset of puberty in intact male mice, but not in castrated males or intact female mice. In addition, human CG increased kidney size in hypophysectomized male mice, but not in castrated hypophysectomized animals. These findings indicate that LH isoforms may regulate kidney growth in the male mouse both directly as a renotropin stimulating hyperplasia and indirectly as a gonadotropin via testicular androgen, producing cellular hypertrophy. It was also noted that G100-fr.3 decreased hepatic weight, DNA, RNA and protein, but produced no significant change in the spleen, heart or adrenal glands in castrated-hypophysectomized mice. Such extragonadal effects of G100-fr.3 were also observed in intact female mice. These results suggest that certain LH isoforms may have extragonadal actions involving the kidney and liver.
authors: Department of Medicine, Vanderbilt University, Nashville, Tennessee.
authors: K
authors: K
authors: Nomura
authors: D W
authors: DW
authors: Puett
authors: D
authors: D
authors: Puett
authors: K
authors: K
authors: Shizume
authors: G W
authors: GW
authors: Liddle
mesh_terms: D000315:Adrenalectomy; D000818:Animals; D004247:DNA / Q000378:metabolism; D005260:Female; D007016:Hypophysectomy; D007668:Kidney / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D007986:Luteinizing Hormone / Q000008:administration & dosage*; D051379:Mice; D008815:Mice, Inbred Strains; D009929:Organ Size / Q000187:drug effects; D010052:Ovariectomy; D011506:Proteins / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D013965:Thyroidectomy
chemical_list: D011506:Proteins; D012313:RNA; D007986:Luteinizing Hormone; D004247:DNA