[Detection of unstable hemoglobins with the use of normal butyl alcohol].
A new screening method for the detection of unstable hemoglobin carriership is suggested, whose characteristic feature is the use of microquantities of red cells, and not of red cell hemolysate; the red cells are added to the specially prepared mixture, 6.5-5.5% by n-butanol volume in phosphate buffer at 18-25 degrees C. Unstable hemoglobin carriership is recorded during 0.5-1 hour from the examined solution turbidity or by measuring the dispersion at 700 nm vs. the transparent control.
Molchanova
T P
TP
D000440:Butanols; D006453:Hemoglobinopathies / Q000175:diagnosis* / Q000235:genetics; D006455:Hemoglobins, Abnormal / Q000032:analysis*; D006801:Humans; D007202:Indicators and Reagents; D009391:Nephelometry and Turbidimetry; D010375:Pedigree; D013057:Spectrum Analysis
D000440:Butanols; D006455:Hemoglobins, Abnormal; D007202:Indicators and Reagents
Vyiavlenie nestabil'nykh gemoglobinov s ispol'zovaniem normal'nogo butilovogo spirta.