title: Optimal temperature for synthesis of DNA, RNA, and protein by human testis in vitro.
abstract: To determine the optimal temperature for DNA, RNA, and protein syntheses in the human testis, the levels of incorporation of 3H-thymidine, 14C-uridine, and 14C-leucine into testicular tissue were studied at 28 degrees C, 31 degrees C, 34 degrees C, and 37 degrees C in vitro. The results suggest that the DNA synthesis is at a maximum at 31 degrees C and shows delicate temperature sensitivity, whereas the RNA and protein syntheses are at a maximum at 34 degrees C or 37 degrees C and are temperature-dependent. It is concluded that the temperature sensitivity of DNA synthesis may be one of the primary causes of delicate thermal inhibition of human spermatogenesis.
authors: Department of Urology, School of Medicine, Osaka University, Japan.
authors: M
authors: M
authors: Nakamura
authors: M
authors: M
authors: Namiki
authors: A
authors: A
authors: Okuyama
authors: E
authors: E
authors: Koh
authors: N
authors: N
authors: Kondoh
authors: M
authors: M
authors: Takeyama
authors: H
authors: H
authors: Fujioka
authors: Y
authors: Y
authors: Nishimune
authors: K
authors: K
authors: Matsumoto
authors: M
authors: M
authors: Matsuda
mesh_terms: D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D006801:Humans; D066298:In Vitro Techniques; D007930:Leucine / Q000378:metabolism; D008297:Male; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*; D013696:Temperature; D013737:Testis / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
chemical_list: D012313:RNA; D004247:DNA; D007930:Leucine; D013936:Thymidine; D014529:Uridine