Analysis of stress-induced gene expression in fish cell lines exposed to heavy meals and heat shock.
We have examined the effect of heavy metals on the expression of two major groups of stress-induced proteins in fish cell lines: the 70 kDa heat-shock proteins (hsp70) and metallothioneins (MTs). The rainbow trout hepatoma (RTH) cell line synthesized the hsp70 protein in response to zinc and heat shock, while chinook salmon embryonic (CHSE) cells synthesized this protein in response to these inducers, as well as cadmium. The synthesis of this 70 kDa protein was correlated with the accumulation of hsp70 mRNA as measured by hybridization to a trout hsp70 gene probe. Heavy metals also induced the synthesis of MT in RTH cells. However, heat shock did not result in induction of MT and its mRNA. Unlike RTH cells, CHSE cells did not synthesize MT following exposure to cadmium or zinc. When these cells were treated with 5-azacytidine prior to heavy metal treatment, accumulation of MT mRNA was observed. Northern blot analysis of total RNA from 5-azacytidine treated CHSE cells, using a trout MT (tMT-B) cDNA probe, indicated that the time-course of induction and the maximal level of MT mRNA accumulation in response to cadmium and zinc paralleled that observed in RTH cells. Copper and dexamethasone were ineffective in inducing MT mRNA in 5-azacytidine-treated CHSE cells. These results indicate that MT is specifically induced in response to heavy metal treatment, whereas the synthesis of hsp70 appears to be a general stress response. Furthermore, MT is differentially regulated by heavy metals and dexamethasone in these cell lines and the expression of MT is cell-type-specific.
Misra
S
S
Department of Biological Sciences, University of Calgary, Victoria, Canada.
Zafarullah
M
M
Price-Haughey
J
J
Gedamu
L
L
D000818:Animals; D001374:Azacitidine / Q000494:pharmacology; D015152:Blotting, Northern; D002104:Cadmium / Q000494:pharmacology*; D002460:Cell Line; D005399:Fishes / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000235:genetics*; D006358:Hot Temperature; D008668:Metallothionein / Q000235:genetics*; D013997:Time Factors; D015032:Zinc / Q000494:pharmacology*
D006360:Heat-Shock Proteins; D002104:Cadmium; D008668:Metallothionein; D015032:Zinc; D001374:Azacitidine