Involvement of tryptase-related cellular protease(s) in human immunodeficiency virus type 1 infection.
Trypstatin, a new cellular Kunitz-type protease inhibitor purified from rat mast cells, inhibited syncytium formation in human immunodeficiency virus type 1 (HIV-1)-infected CCRF-CEM and uninfected Molt-4 clone 8 at a concentration of 1 microM. Anti-rat tongue mast cell tryptase antibodies reacted with Molt-4 clone 8 cells, as determined by Western blot and by immunofluorescence. In addition, the antibody inhibited syncytium formation. These findings along with homologous sequences with trypstatin and a neutralizing epitope of gp120 of HIV-1 suggest that a tryptase-like cellular enzyme(s) is involved in HIV-1 infection.
Hattori
T
T
Second Department of Internal Medicine, Kumamoto University Medical School, Japan.
Koito
A
A
Takatsuki
K
K
Kido
H
H
Katunuma
N
N
D000163:Acquired Immunodeficiency Syndrome / Q000201:enzymology*; D000510:Alpha-Globulins; D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000494:pharmacology*; D000954:Antigens, Surface / Q000032:analysis; D015153:Blotting, Western; D002478:Cells, Cultured; D000939:Epitopes / Q000032:analysis; D015497:HIV-1 / Q000201:enzymology*; D006801:Humans; D008407:Mast Cells / Q000032:analysis; D009500:Neutralization Tests; D010447:Peptide Hydrolases / Q000276:immunology*; D011480:Protease Inhibitors / Q000494:pharmacology*; D011506:Proteins / Q000032:analysis / Q000494:pharmacology*; D051381:Rats; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000382:microbiology; D014059:Tongue / Q000201:enzymology
D000510:Alpha-Globulins; D000906:Antibodies; D000954:Antigens, Surface; D000939:Epitopes; D011480:Protease Inhibitors; D011506:Proteins; C016001:alpha-1-microglobulin; D010447:Peptide Hydrolases; C023073:tosylarginine methyl ester hydrolase