[Methodology of determining Yersinia pestis antigens using dot-immunologic analysis].
The technique of dot-immunoassay on nitrocellulose filters is described for the detection of the vital infection (VI) agents. The results are read visually (qualitatively) and instrumentally (quantitatively). The method is highly sensitive (3.2 +/- 0.8 ng/ml of fraction I) and specific, cost-effective, simple, and available for laboratories engaged in VI diagnosis.
Polunina
T A
TA
Korovkin
S A
SA
Naumov
A V
AV
D000942:Antigens, Bacterial / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D015010:Yersinia pestis / Q000276:immunology*
D000942:Antigens, Bacterial
Metodika opredeleniia antigenov chumnogo mikroba s pomoshch'iu dot-immunologicheskogo analiza.