Hydrocortisone-induced accumulation of fibronectin mRNA and cell surface-associated fibronectin.
A Morris hepatoma 7777-derived cell line, DTH-3, was used to study the control of fibronectin gene expression. In cultures of DTH-3 cells in conventional medium supplemented with serum or in chemically defined MX-83 medium supplemented with insulin no cell surface fibronectin was detectable by indirect immunofluorescence techniques using specific polyclonal antibodies. By Northern blot hybridization analysis a dose- and time-dependent accumulation of 8 kb fibronectin mRNA in response to hydrocortisone treatment was demonstrated. Furthermore, 24 h after addition of hydrocortisone an extensive fibrillar fibronectin network was established. The results suggest that the hydrocortisone-dependent induction of fibronectin production might, at least in part, be controlled at the transcriptional level.
Begemann
M
M
Frauenhofer Institut für Toxikologie, Hannover, Federal Republic of Germany.
Voss
B
B
Paul
D
D
D000818:Animals; D005353:Fibronectins / Q000096:biosynthesis / Q000235:genetics*; D006854:Hydrocortisone / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D014407:Tumor Cells, Cultured; D000509:alpha-Fetoproteins / Q000235:genetics
D005353:Fibronectins; D012333:RNA, Messenger; D000509:alpha-Fetoproteins; D006854:Hydrocortisone