Regulation of muscle acetylcholine receptor-channel function by interferon.
The effect of the antiviral agent interferon (IFN) on the function of the nicotinic acetylcholine receptor (AChR) channel, has been investigated in both mammalian cultured myotubes and adult fibres, using the single channel recording patch-clamp technique. Shortened AChR-channel lifetime, and occasionally reduced channel conductance and slowed opening frequency were seen with fibroblast IFN (IFN-beta) in the mouse myotubes, and with IFN-beta and leucocytes IFN (IFN-alpha), in the rat muscle fibres. These effects paralleled an increase in the cytosolic level of cAMP. This suggests that IFN exerts a regulatory action on AChR function. A similar regulatory action on other receptor may be responsible for some of the neurological side effects observed in patients treated with IFN.
Eusebi
F
F
Dipartimento di Medicina Sperimentale, Università dell'Aquila, Roma, Italy.
Farini
D
D
Grassi
F
F
Santoni
A
A
D000818:Animals; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000096:biosynthesis; D004553:Electric Conductivity; D004622:Embryo, Mammalian; D007370:Interferon Type I / Q000494:pharmacology*; D007473:Ion Channels / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009132:Muscles / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D011978:Receptors, Nicotinic / Q000502:physiology*; D011994:Recombinant Proteins
D007370:Interferon Type I; D007473:Ion Channels; D011978:Receptors, Nicotinic; D011994:Recombinant Proteins; D000242:Cyclic AMP