Harmine as a substitute for 33258 Hoechst in the FPG technique.
We studied the effectiveness of harmine as a substitute for 33258 Hoechst in the fluorescence-plus-Giemsa technique, using Allium cepa chromosomes after 5-bromo-2'-deoxyuridine (BrdU) incorporation. Harmine showed a photosensitizing capacity which was somewhat higher than 33258 Hoechst and used half of the time established for the usual treatment.
Gutiérrez-Gonzálvez
M G
MG
Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
Hazen
M J
MJ
Espelosín
R H
RH
D000470:Alkaloids; D000490:Allium; D001399:Azure Stains; D006690:Bisbenzimidazole; D001973:Bromodeoxyuridine / Q000378:metabolism; D002842:Chromatids / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D006247:Harmine; D006651:Histocytochemistry / Q000379:methods*; D013050:Spectrometry, Fluorescence
D000470:Alkaloids; D001399:Azure Stains; D006247:Harmine; D004247:DNA; D001973:Bromodeoxyuridine; D006690:Bisbenzimidazole