Quantitation of cutaneous inflammation induced by reactive species generated by UV-visible irradiation of rose bengal.
The present studies were undertaken to quantitate the initial inflammatory response produced by the photo-generated reactive species in rabbit skin. Rose bengal (RB), a photosensitizer dye, was injected into the skin sites at various concentrations and exposed to UV-visible light for 30-120 min. The increase in vascular permeability and the accumulation of PMNs were investigated using 125I-labeled albumin and 51Cr-labeled PMNs. RB at a concentration of 1 nmol with 120-min exposure to light enhanced vascular permeability by 3.7 times and accumulation of PMNs by 3.3 times. As low as 0.01 nmol of RB produced discernible effects. beta-Carotene (0.1 nmole) inhibited the inflammatory response by 75-100%, suggesting that the reactive species involved in this response was predominantly singlet oxygen. The increase in vascular permeability was inhibited by 48-70% by 25 micrograms of chlorpheniramine maleate. It is therefore suggested that histamine plays a major role in the initial vascular response. The studies demonstrate that this rabbit model is suitable for the quantitation of photoinduced inflammatory response which is not observable by gross anatomic procedures.
Ranadive
N S
NS
Department of Pathology, University of Toronto, Ontario, Canada.
Menon
I A
IA
Shirwadkar
S
S
Persad
S D
SD
D000818:Animals; D002199:Capillary Permeability; D002338:Carotenoids / Q000494:pharmacology; D002744:Chlorpheniramine / Q000494:pharmacology; D004195:Disease Models, Animal; D005609:Free Radicals; D006636:Histamine Release / Q000187:drug effects; D009504:Neutrophils / Q000473:pathology; D010100:Oxygen / Q000633:toxicity; D010787:Photosensitivity Disorders / Q000139:chemically induced / Q000473:pathology*; D011817:Rabbits; D012395:Rose Bengal / Q000528:radiation effects / Q000633:toxicity*; D026082:Singlet Oxygen; D012867:Skin / Q000098:blood supply; D014466:Ultraviolet Rays; D019207:beta Carotene
D005609:Free Radicals; D019207:beta Carotene; D026082:Singlet Oxygen; D012395:Rose Bengal; D002338:Carotenoids; D002744:Chlorpheniramine; D010100:Oxygen