Detection of a gamma interferon-induced protein IP-10 in psoriatic plaques.
The pathologic features of psoriatic plaques are inflammation and increased epidermal turnover. IP-10, a cytokine the expression of which is induced by gamma-interferon, is a member of a family of soluble mediators with inflammatory and growth-promoting activities. IP-10 protein was detected in keratinocytes and the dermal infiltrate from active psoriatic plaques using an affinity-purified rabbit anti-IP-10 antibody in immunoperoxidase studies. Successful treatment of active plaques decreased IP-10 expression in plaques. These results were corroborated by Northern blot analysis with an IP-10 cDNA probe. We have previously detected activated T cells and HLA-DR keratinocytes in active psoriatic plaques. Since IP-10 is detected in delayed cellular immune responses, the present study further points to the role of ongoing cellular immune responses in the pathogenesis of psoriasis.
Gottlieb
A B
AB
Laboratory of Immunology, Rockefeller University, New York.
Luster
A D
AD
Posnett
D N
DN
Carter
D M
DM
D015152:Blotting, Northern; D054357:Chemokine CXCL10; D019743:Chemokines, CXC; D004817:Epidermis / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D007371:Interferon-gamma / Q000494:pharmacology; D007633:Keratins; D011506:Proteins / Q000378:metabolism*; D011565:Psoriasis / Q000503:physiopathology*; D012333:RNA, Messenger / Q000378:metabolism
D054357:Chemokine CXCL10; D019743:Chemokines, CXC; D011506:Proteins; D012333:RNA, Messenger; D007633:Keratins; D007371:Interferon-gamma