Column chromatographic separation of cells using aqueous polymeric two-phase systems.
Cell separation using aqueous polymeric two-phase systems is well established. For separations of cells having similar partition coefficients a multistep countercurrent distribution procedure has to be used. However, its operation is limited by time and apparatus constraints. As an alternative strategy we have developed a chromatographic technique in which the dextran-rich phase of a dextran/polyethylene glycol (PEG) phase system is immobilized onto derivatized agarose beads. The PEG-rich phase is used as the eluent. Inclusion of PEG-fatty acid affinity ligand gradients into the eluent produces separations of mammalian erythrocytes based on the differential interaction between the fatty acid and the erythrocyte membranes. A model separation of dog and human erythrocytes has been carried out.
Skuse
D R
DR
Department of Chemistry, University of British Columbia, Vancouver, Canada.
Müller
W
W
Brooks
D E
DE
D002469:Cell Separation; D003911:Dextrans; D004912:Erythrocytes / Q000166:cytology; D005227:Fatty Acids; D011092:Polyethylene Glycols; D012685:Sepharose
D003911:Dextrans; D005227:Fatty Acids; D011092:Polyethylene Glycols; D012685:Sepharose