A comparative study of protein synthesis by keratinocytes and fibroblasts in vitro using two-dimensional gel electrophoresis and dual isotope autoradiography.
A procedure is described for the detection of keratinocyte specific proteins. Fibroblasts and keratinocytes were isolated from human skin and radiolabelled in vitro. Samples were separated by two-dimensional polyacrylamide gel electrophoresis to compare the proteins synthesised by the different types of cultured skin cells. Dual label autoradiography of samples radiolabelled with [35S]methionine and [75Se]selenomethionine was used to identify keratinocyte specific proteins. We report 45 keratinocyte-specific components and identify some of these proteins. The differential expression of these proteins and their relevance to epidermal differentiation are discussed.
Easty
D J
DJ
Department of Histopathology, Royal Postgraduate Medical School, London, UK.
Patel
K
K
Dover
R
R
Evans
D J
DJ
Dunn
M J
MJ
D001345:Autoradiography; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007633:Keratins / Q000378:metabolism*; D014176:Protein Biosynthesis
D007633:Keratins