Detection of retrovirus particles and reverse transcriptase activity in mid-term cultured peripheral blood and lymph node cells from a French woman with Sezary syndrome.
Retrovirus particles, with an ultrastructure of type C-virus similar to HTLV-I were observed in several mid-term cultures of leukemic cells derived from a woman with a well characterised Sezary syndrome who had always resided in France. Reverse transcriptase activity was detected in supernatant fluids from day 6 to day 40 of culture. However, negative anti HTLV-I serology and the absence of specific molecular hybridization between leukemic cell DNA and two HTLV-I derived probes, argue against a HTLV-I virus.
Saal
F
F
UPR A0043 CNRS Rétrovirus et Rétrotransposons des Vertébrés, Hôpital Saint-Louis, Paris, France.
Gessain
A
A
Lasneret
J
J
Foldès
C
C
Dronne
P
P
Wallach
D
D
Cottenot
F
F
Sigaux
F
F
Flandrin
G
G
Périès
J
J
D000368:Aged; D015139:Blotting, Southern; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics; D005260:Female; D005602:France; D015368:Human T-lymphotropic virus 1 / Q000276:immunology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000382:microbiology*; D008198:Lymph Nodes / Q000382:microbiology*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D012190:Retroviridae / Q000302:isolation & purification*; D012751:Sezary Syndrome / Q000382:microbiology*; D014771:Virion / Q000302:isolation & purification
D004247:DNA; D012194:RNA-Directed DNA Polymerase