Effectiveness of hMG-hCG treatment for DNA/RNA syntheses in subfertile human testis in vitro.
To evaluate the effect of hMG-hCG treatment on spermatogenic impairment, DNA/RNA syntheses were measured in 25 patients with idiopathic male infertility by estimating the values of 3H-thymidine and 14C-uridine incorporation into the testicular tissue cultured with or without the hormonal treatment. Without the hormonal treatment, the levels of 3H-thymidine incorporation were in inverse correlation with the progress of spermatogenic impairment, whereas disturbances in responsiveness to the hormonal treatment directly correlated with spermatogenic impairment. Although the 14C-uridine incorporation increased both with and without the hormonal treatment, the values showed different patterns from those of 3H-thymidine incorporation. It would appear that the DNA synthesis in testicular tissue cultured with or without hMG-hCG treatment might predict the responsiveness to hormonal treatment for patients with idiopathic male infertility.
Okuyama
A
A
Department of Urology and Pathology, Osaka University Medical School, Japan.
Nonomura
N
N
Nakamura
M
M
Namiki
M
M
Koh
E
E
Kondoh
N
N
Kiyohara
H
H
Nishimune
Y
Y
Matsumoto
K
K
Sonoda
T
T
D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D046508:Culture Techniques; D004247:DNA / Q000096:biosynthesis*; D005640:Follicle Stimulating Hormone / Q000097:blood; D007248:Infertility, Male / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D008596:Menotropins / Q000494:pharmacology*; D012313:RNA / Q000096:biosynthesis*; D013737:Testis / Q000187:drug effects* / Q000378:metabolism
D006063:Chorionic Gonadotropin; D008596:Menotropins; D012313:RNA; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D004247:DNA