Evaluation of an immunoluminometric assay for the AFP determination.
We evaluated a two-site immunoluminometric assay (AFP LIA-mat byk) for the alpha-fetoprotein (AFP) determination. The assay, involving two monoclonal antibodies which recognize two different epitopes of the AFP, is rapid (approximately 4 h), with a wide working range (0-600 IU/ml) and a good sensitivity of detection. It presents a good reproducibility (CV less than 10% for the total working range). The regression equation for the AFP LIA-mat (Y) and the AFP bridge Serono (X) was Y = 1.175X-2.27 (n = 95, r = 0.996). It didn't present hook effect and, when we assessed the linearity by assaying a high concentration of AFP, it gave a linear response down to 3.5 IU/ml.
Boreau
M
M
Villalta
D
D
Santini
G F
GF
D000911:Antibodies, Monoclonal; D000939:Epitopes / Q000032:analysis; D006801:Humans; D007118:Immunoassay / Q000379:methods; D015592:Immunoradiometric Assay; D008165:Luminol / Q000031:analogs & derivatives; D011724:Pyridazines; D011933:Reagent Kits, Diagnostic; D013223:Statistics as Topic; D000509:alpha-Fetoproteins / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D011724:Pyridazines; D011933:Reagent Kits, Diagnostic; D000509:alpha-Fetoproteins; C032487:isoluminol; D008165:Luminol