Various proteins from rat brain, specifically and irreversibly labeled by [3H]flunitrazepam, are distinct alpha-subunits of the GABA-benzodiazepine receptor complex.
gamma-Aminobutyric acid (GABA)-benzodiazepine receptors were purified from the brains of 5 to 10-day-old rats and photolabeled by [3H]flunitrazepam. After sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), 3 different proteins with apparent molecular weight 51,000, 53,000 and 59,000 Da specifically and irreversibly labeled by [3H]flunitrazepam were revealed by autoradiography. The same 3 proteins were recognized by the alpha-subunit-specific monoclonal antibody bd-28. In contrast, a different protein with apparent molecular weight 56,000 Da was recognized by the beta-subunit-specific antibody bd-17. These results indicate that the various proteins specifically labeled by [3H]flunitrazepam are different alpha-subunits and distinct and different from the beta-subunit of the GABA-benzodiazepine receptor complex.
Fuchs
K
K
Department of Biochemical Psychiatry, Psychiatrische Universitätsklinik, Vienna, Austria.
Möhler
H
H
Sieghart
W
W
D000818:Animals; D000911:Antibodies, Monoclonal; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry; D000939:Epitopes; D005445:Flunitrazepam / Q000378:metabolism*; D008970:Molecular Weight; D051381:Rats; D011963:Receptors, GABA-A / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000939:Epitopes; D011963:Receptors, GABA-A; D005445:Flunitrazepam