A simple and efficient method of measuring in vitro amylase secretion by dispersed pancreatic acini.
A method of measuring amylase secretion from dispersed pancreatic acini is described that utilizes a 20-micron nylon mesh to separate cell-associated from secreted amylase. When compared with the standard centrifugation assay, the mesh technique permits easy measurement at multiple, closely spaced time points while reducing the number of samples generated and allowing rapid manipulation of the extracellular suspending medium.
Rutledge
P L
PL
Department of Surgery, Beth Israel Hospital, Boston, Massachusetts 02215.
Saluja
A
A
Leli
U
U
Steer
M L
ML
D000681:Amylases / Q000378:metabolism*; D002498:Centrifugation; D066298:In Vitro Techniques; D008722:Methods; D010179:Pancreas / Q000201:enzymology* / Q000378:metabolism
D000681:Amylases