Expression of parathyroid hormone-related protein in a human T cell lymphotrophic virus type I-infected T cell line.
We analyzed human T cell lymphotrophic virus type I (HTLV-I)-infected T cells for the presence of mRNA coding for parathyroid hormone-related protein (PTHrP) by Northern blotting using synthetic DNA probes. We report here that PTHrP mRNAs were detected in a HTLV-I-infected T cell line, MT-2, but not in uninfected T cell or B cell lines, and that PTH-like bioactivity was detected only in the conditioned medium of MT-2 cells. Our study suggests that the pathophysiology of hypercalcemia in patients with adult T cell leukemia/lymphoma may resemble that which occurs with solid tumors.
Motokura
T
T
Fourth Department of Internal Medicine, University of Tokyo School of Medicine, Japan.
Fukumoto
S
S
Takahashi
S
S
Watanabe
T
T
Matsumoto
T
T
Igarashi
T
T
Ogata
E
E
D002460:Cell Line; D002472:Cell Transformation, Viral; D017977:Deltaretrovirus / Q000235:genetics*; D006410:Hematopoiesis; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics*; D010281:Parathyroid Hormone / Q000235:genetics*; D044162:Parathyroid Hormone-Related Protein; D011061:Poly A / Q000235:genetics; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D013601:T-Lymphocytes
D009363:Neoplasm Proteins; C470121:PTHLH protein, human; D010281:Parathyroid Hormone; D044162:Parathyroid Hormone-Related Protein; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA