Characterization of receptor for granulocyte colony-stimulating factor on human circulating neutrophils.
We have made a mutein of human G-CSF with more stable, and potent biological activity. Using 125 I-labeled mutein human G-CSF, high affinity binding sites were identified on human circulating neutrophils. Receptor number per cell was 560 with a Kd of 250 pM. The human G-CSF receptor was identified as a single subunit protein of Mr approximately 150,000.
Uzumaki
H
H
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Okabe
T
T
Sasaki
N
N
Hagiwara
K
K
Takaku
F
F
Itoh
S
S
D003115:Colony-Stimulating Factors / Q000378:metabolism*; D003432:Cross-Linking Reagents; D016179:Granulocyte Colony-Stimulating Factor; D006801:Humans; D007457:Iodine Radioisotopes; D007700:Kinetics; D008970:Molecular Weight; D009504:Neutrophils / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D016188:Receptors, Granulocyte Colony-Stimulating Factor; D013696:Temperature
D003115:Colony-Stimulating Factors; D003432:Cross-Linking Reagents; D007457:Iodine Radioisotopes; D011956:Receptors, Cell Surface; D016188:Receptors, Granulocyte Colony-Stimulating Factor; D016179:Granulocyte Colony-Stimulating Factor