[Effect of salvin on the incorporation of labeled precursors into macromolecular compounds of Staphylococcus aureus 209P].
Salvin is a preparation of Salvia officinalis L. Its effect on synthesis of macromolecules in cells of Staphylococcus aureus 209P was studied with labeled precursors in a system used for investigation of peptidoglycan synthesis. At a concentration of 10 micrograms/ml salvin inhibited incorporation of 14C-lysine into the cell wall polymer and protein fraction by 42.9 and 8.9 per cent respectively and stimulated incorporation of 3H-thymidine and 3H-uridine into the nucleic acid fraction. In the presence of salvin in a quantity of 120 micrograms/ml there was observed inhibition of 3H-uridine incorporation into the nucleic acid fraction by 53.3 per cent and 14C-lysine into the protein fraction by 74.5 per cent along with inhibition of peptidoglycan synthesis by 95.5 per cent. The results conformed to the findings of electron microscopic investigation of the solving effect on ultrastructure of S. aureus 209P. They confirmed the previous assumption that salvin had the primary effect on the processes directly associated with synthesis of the cell wall polymer.
Pavlenko
L V
LV
Mashkovskiĭ
N N
NN
Smirnov
V V
VV
D045784:Abietanes; D002250:Carbon Radioisotopes; D004269:DNA, Bacterial / Q000378:metabolism*; D004224:Diterpenes; D066298:In Vitro Techniques; D008239:Lysine / Q000378:metabolism; D010457:Peptidoglycan / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D010616:Phenanthrenes / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D012329:RNA, Bacterial / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D045784:Abietanes; D002250:Carbon Radioisotopes; D004269:DNA, Bacterial; D004224:Diterpenes; D010457:Peptidoglycan; D010616:Phenanthrenes; D010936:Plant Extracts; D012329:RNA, Bacterial; D014316:Tritium; D008239:Lysine; C018381:salvin; D013936:Thymidine; D014529:Uridine
Vliianie sa'lvina na vkliuchenie mechenykh predshestvennikov v makromolekuliarnye soedineniia Staphylococcus aureus 209P.