title: Retrotransposon transposition intermediates are encapsidated into virus-like particles.
abstract: Virus-like particles (VLPs) possessing reverse transcriptase activity are persistently present in Drosophila melanogaster cultured cells and are formed in yeast induced for transposition. Different retrotransposon transposition intermediates consistent with those expected from the model of reverse transcription pathway of retrotransposon transposition have been detected during the analysis of nucleic acids isolated from VLPs. These data indicate that the act of reverse transcription takes place in VLPs which may be considered as functional intermediates of transposition.
authors: N.I. Vavilov Institute of General Genetics, Moscow, USSR.
authors: T V
authors: TV
authors: Gorelova
authors: N L
authors: NL
authors: Resnick
authors: N G
authors: NG
authors: Schuppe
mesh_terms: D000818:Animals; D002460:Cell Line; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000382:microbiology; D010957:Plasmids; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014170:Transformation, Genetic; D014780:Viruses / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004251:DNA Transposable Elements; D012194:RNA-Directed DNA Polymerase