Epitopes on the major capsid protein of simian virus 40.
Thirteen monoclonal antibodies which react with the major capsid protein (VP1) of simian virus 40 (SV40) have been isolated. Of these, five neutralized viral infectivity when added in sufficient concentration. Seven of the antibodies reacted with denatured VP1 and also recognized fragments generated by protease or cyanogen bromide cleavage. The region of VP1 recognized by all seven antibodies was mapped within a nine-amino-acid segment located in the carboxyl portion of the protein (from amino acid positions 312 to 321). This region is likely to protrude from the surface of the protein as judged by high hydrophilicity and low hydropathy predicted from the amino acid sequence and lack of secondary structure by contrast with the rest of the protein for which predominantly beta-sheet structure is predicted. Competition between these antibodies and synthetic peptides for binding to virus particles confirmed that the continuous epitope is contained within the nine-amino-acid sequence. Competition between the different monoclonal antibodies suggested that the continuous epitope was also part of more complex discontinuous epitopes recognized by some of the other antibodies. These results support a model in which a segment of the carboxyl-terminal portion of VP1 protrudes from the surface of the virus to form an antigenic structure.
Babé
L M
LM
Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Florida 33101.
Brew
K
K
Matsuura
S E
SE
Scott
W A
WA
D000465:Algorithms; D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000936:Antigen-Antibody Complex; D001667:Binding, Competitive; D002213:Capsid / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D011487:Protein Conformation; D013539:Simian virus 40 / Q000276:immunology*; D012984:Software
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000939:Epitopes