title: DNA repair after gamma irradiation in lymphocytes exposed to low-frequency pulsed electromagnetic fields.
abstract: The effect of exposure to extremely low-frequency pulsed electromagnetic fields (EMFs) on DNA repair capability and on cell survival in human lymphocytes damaged in vitro with gamma rays was studied by two different micromethods. In the first assay, which measures DNA repair synthesis (unscheduled DNA synthesis, UDS), lymphocyte cultures were stimulated with phytohemagglutinin (PHA) for 66 h and then treated with hydroxyurea (which blocks DNA replication), irradiated with 100 Gy of 60Co, pulsed with [3H]thymidine ([3H]TdR), and then exposed to pulsed EMFs for 6 h (the period in which cells repaired DNA damage). In the second assay, which measures cell survival after radiation or chemical damage, lymphocytes were first irradiated with graded doses of gamma rays or treated with diverse antiproliferative agents, and then stimulated with PHA, cultured for 72 h, and pulsed with [3H]TdR for the last 6 h of culture. In this case, immediately after the damage induced by either the radiation or chemicals, cultures were exposed to pulsed EMFs for 72 h, during which cell proliferation took place. Exposure to pulsed EMFs did not affect either UDS or cell survival, suggesting that this type of nonionizing radiation--to which humans may be exposed in the environment, and which is used for both diagnostic and therapeutic purposes--does not affect DNA repair mechanisms.
authors: Institute of General Pathology, University of Modena, Italy.
authors: A
authors: A
authors: Cossarizza
authors: D
authors: D
authors: Monti
authors: P
authors: P
authors: Sola
authors: G
authors: G
authors: Moschini
authors: R
authors: R
authors: Cadossi
authors: F
authors: F
authors: Bersani
authors: C
authors: C
authors: Franceschi
mesh_terms: D000293:Adolescent; D000328:Adult; D001761:Bleomycin / Q000009:adverse effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D003037:Cobalt Radioisotopes; D004247:DNA / Q000528:radiation effects*; D004260:DNA Repair; D004317:Doxorubicin / Q000009:adverse effects; D004574:Electromagnetic Fields / Q000009:adverse effects*; D055590:Electromagnetic Phenomena / Q000009:adverse effects*; D005720:Gamma Rays; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects / Q000528:radiation effects*; D008875:Middle Aged; D014747:Vinblastine / Q000009:adverse effects
chemical_list: D003037:Cobalt Radioisotopes; D001761:Bleomycin; D014747:Vinblastine; D004317:Doxorubicin; D004247:DNA