title: Phenotypic expression of proteoglycan in mast cells of the human nasal mucosa.
abstract: The phenotypic expression of the proteoglycan of human mast cells in the nasal mucosa and normal skin was analysed using histochemical techniques. Nasal mucosa was obtained from normal subjects, from patients with seasonal allergic rhinitis before and during the pollen season and from patients with nasal polyps. In the latter groups, specimens were taken from both polyp tissue and adjacent nasal mucosa. Formaldehyde treatment blocked the cationic dye binding in 75-84% of the mast cells located in the nasal mucosa, as compared to the optimum fixation with IFAA (iso-osmotic formaldehyde-acetic acid). A significantly lower degree of blocking of dye binding was obtained in the human skin where 45% of the mast cells were susceptible to formaldehyde treatment (P less than 0.01). The mast cells of the polyp tissue also showed a relatively low degree of blocking (54%), which was significantly lower than the blocking of mast cells of the nasal mucosa taken from the same individuals (P less than 0.05). Staining of serial tissue sections in Alcian Blue containing graded concentrations of MgCl2 was used to determine the critical electrolyte concentration (CEC) of the dye binding, defined as the salt concentration at which the staining of 50% of the mast cells is extinguished. The CEC of the skin mast cells was 0.64M MgCl2 which is significantly higher than that of the mast cells of the nasal mucosa of normal subjects [0.49M (P less than 0.05)], allergic subjects [0.52M (P less than 0.01)], patients with polyp disease [0.52M (P less than 0.01)] and the polyp tissue proper [0.57M (P less than 0.05)].(ABSTRACT TRUNCATED AT 250 WORDS)
authors: Department of Pathology, Sahlgrenska Hospital, Gothenburg University, Sweden.
authors: U
authors: U
authors: Pipkorn
authors: G
authors: G
authors: Karlsson
authors: L
authors: L
authors: Enerbäck
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001599:Berberine / Q000378:metabolism; D005260:Female; D005456:Fluorescent Dyes / Q000378:metabolism; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D008297:Male; D008407:Mast Cells / Q000032:analysis*; D008875:Middle Aged; D009297:Nasal Mucosa / Q000032:analysis*; D010641:Phenotype; D011509:Proteoglycans / Q000235:genetics*; D013194:Staining and Labeling / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D011509:Proteoglycans; D001599:Berberine