A space-resolved fluorometer and its application to immunoassay.
A metal surface has been added into a fluorometer to reflect the excited light and to reduce the light scattering. Due to the total separation of the fluorescent signal and the noise by directing them into different spaces, the resolution could be improved by several orders of magnitude. The same metal surface could also be used for immunoassays. The detection of alpha-fetoprotein in normal rat serum and human standard serum are given as examples. The results indicate that the apparatus and the method have significant advantages.
Wang
W K
WK
Institute of Physics, Academia Sinica, Taipei, Taiwan, China.
Ho
L T
LT
Chiang
Y
Y
Chen
T C
TC
D000888:Antibodies, Anti-Idiotypic / Q000032:analysis; D005455:Fluorescent Antibody Technique; D005470:Fluorometry / Q000295:instrumentation*; D006801:Humans; D000509:alpha-Fetoproteins / Q000032:analysis
D000888:Antibodies, Anti-Idiotypic; D000509:alpha-Fetoproteins