title: Epithelium-specific response of cultured keratinocytes to infection with adenovirus type 2.
abstract: Adenoviruses are pathogenic for certain stratified squamous epithelia. The sites most frequently involved are the upper respiratory tract and oropharynx. Adenovirus infections of the epidermis are quite rare. We examined the virus-cell interactions of adenovirus type 2 (Ad2) and cultured human keratinocytes grown from a variety of body sites. Our intent was to explore the nature of the apparent epithelium-specific susceptibility to Ad2. In brief, we found that in vitro viral susceptibility of the keratinocytes could be reliably predicted based on whether the cells originated from an epidermal or oropharyngeal surface. Ad2 proceeded through a complete vegetative cycle when used to infect cultured keratinocytes from oropharyngeal sites (e.g., gingiva and soft palate). In contrast, Ad2 infection was severely restricted in keratinocytes from epidermal sites (e.g., foreskin, abdomen, and buttock). These results demonstrate that the in vitro response to infection with Ad2 reflects in vivo tissue-specific susceptibility. In vivo, cervical epithelium is rarely infected with Ad2 and yet in culture, cervical keratinocytes were fully permissive for Ad2 replication. We propose that the permissive or nonpermissive response to Ad2 may be regulated by a particular aspect of cell phenotype. Because the permissive responses seen in this study were all generated in keratinocytes from mucosal sites, it is possible the in vitro response to Ad2 reflects inherent differences between mucosal and epidermal keratinocytes.
authors: Department of Oral Biology and Pathology, School of Dental Medicine, State University of New York, Stony Brook 11794-8702.
authors: B J
authors: BJ
authors: Aneskievich
authors: L B
authors: LB
authors: Taichman
mesh_terms: D000260:Adenoviruses, Human / Q000254:growth & development / Q000378:metabolism; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D004817:Epidermis / Q000382:microbiology / Q000473:pathology; D004848:Epithelium / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D006801:Humans; D007633:Keratins; D009061:Mouth Mucosa / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D012874:Skin Diseases, Infectious / Q000378:metabolism / Q000382:microbiology / Q000473:pathology*; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
chemical_list: D014764:Viral Proteins; D007633:Keratins