Alpha-actinin synthesis can be modulated by antisense probes and is autoregulated in non-muscle cells.
We used a 279 bp cDNA probe derived from a Dictyostelium alpha-actinin genomic sequence to assay the degree of homology between alpha-actinin from slime molds, mammalian and chicken cells. Recognition of this probe by vertebrate cells was shown in Southern and Northern blots, and by antisense RNA-induced depression of endogenous alpha-actinin synthesis in living cells. Micro-injection of Dictyostelium or chicken gizzard alpha-actinin resulted in incorporation of these proteins in stress fibers, peripheral microfilament belts and adhesion sites. Alpha-actinin-injected cells showed a marked, transient reduction of synthesis of the corresponding endogenous protein. These data emphasize the high degree of conservation of alpha-actinin during evolution and show for the first time autoregulation of synthesis for a microfilament protein.
Schulze
H
H
Developmental Biology Unit, University of Bielefeld, FRG.
Huckriede
A
A
Noegel
A A
AA
Schleicher
M
M
Jockusch
B M
BM
D008841:Actin Cytoskeleton / Q000378:metabolism; D000185:Actinin / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D000818:Animals; D002478:Cells, Cultured; D002645:Chickens; D004023:Dictyostelium / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D006706:Homeostasis; D012313:RNA / Q000494:pharmacology*; D015347:RNA Probes / Q000494:pharmacology*; D016372:RNA, Antisense; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D005656:Fungal Proteins; D015347:RNA Probes; D016372:RNA, Antisense; D000185:Actinin; D012313:RNA