[Antigenic polysaccharides of bacteria. 26. Structure of O-specific polysaccharides from Pseudomonas cerasi 467 and Pseudomonas syringae pv. syringae strains 218 and P-55 belonging to serogroups II and III].
Serologically active O-specific polysaccharides were obtained on mild acid hydrolysis of lipopolysaccharides from Pseudomonas cerasi 467 and Pseudomonas syringae pv. syringae strains 218 and P-55. On the basis of 1H- and 13C-NMR analysis, it was concluded that the P. cerasi polysaccharide has the following structure: ----3)-alpha-D-Rhap-(1----3)-alpha-D-Rhap-(1----2)-alpha-D-+ ++Rhap-(1---- which is identical to that of O-specific polysaccharide from P. syringae pv. morsprunorum C28 (Smith A. R. W. et al. Eur. J. Biochem., 1985, V. 149, No 1, p. 73-78). The polysaccharides from P. syringae pv. syringae strains possess the same backbone but differ by the presence of D-fucose as monosaccharide branches. Methylation and 1H- and 13C-NMR analysis revealed the following structure of these polysaccharides: (Formula: see text). The degree of substitution of the backbone trisaccharide units by the fucofuranose residues is about 35% for the strain 218 and about 85% for the strain P-55.
Knirel'
Iu A
IuA
Zdorovenko
G M
GM
Shashkov
A S
AS
Mamian
S S
SS
Iakovleva
L M
LM
D000942:Antigens, Bacterial / Q000032:analysis*; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000032:analysis* / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy; D019081:O Antigens; D011549:Pseudomonas / Q000145:classification / Q000276:immunology*; D012703:Serotyping
D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D019081:O Antigens
Antigennye polisakharidy bakteriĭ. 26. Stroenie O-spetsificheskikh polisakharidov Pseudomonas cerasi 467 i Pseudomonas syringae, patovar syringae, shtammy 218 i P-55, otnosiashchikhsia k serogruppam II i III.