[A quantitative study of Ag-positive nucleolar segments revealed by silver staining in the interphase nuclei of trophoblast cells from the rat placental connective zone].
A cytomorphological study was made of silver stained nucleoli in interphasic nuclei of trophoblast cells from the rat placenta connective zone, in addition to calculation of Ag-positive spherules in the nucleoli. The prevalent number of Ag-positive nucleolar spherules in the nuclei was 6, corresponding to the number of nucleolar organizers (NOR's) in the diploid chromosome complement of the rat. The mean number of Ag-positive spherules in the nucleoli progressively increase in the course of polyploidization from 2c to 32c; variability of the spherule number also increasing. The mean area of nucleoli is found to increase in proportion to the ploidy degree. A high correlation is found between the number of Ag-positive spherules and the area of nucleoli in the nucleus (r = 0.78). This appropriateness is exhibited at all the ploidy levels. The number of Ag-spherules and the area of nucleoli are found to depend slightly on the number of nucleoli. The possibility to use the number of Ag-positive spherules as a criterion of the activity of the NOR in interphasic nuclei is discussed.
Zybina
T G
TG
Zybina
E V
EV
D000818:Animals; D002466:Cell Nucleolus / Q000648:ultrastructure*; D002467:Cell Nucleus / Q000648:ultrastructure*; D005260:Female; D007399:Interphase; D009697:Nucleolus Organizer Region / Q000648:ultrastructure; D010920:Placenta / Q000648:ultrastructure*; D011123:Polyploidy; D011247:Pregnancy; D051381:Rats; D012835:Silver Nitrate; D013194:Staining and Labeling / Q000379:methods; D014327:Trophoblasts / Q000648:ultrastructure*
D012835:Silver Nitrate
Kolichestvennoe izuchenie Ag-polozhitel'nykh uchastkov iadryshek, vyiavliaemykh v interfaznykh iadrakh kletok trofoblasta soedinitel'noĭ platsenty krysy metodom serebreniia.