title: Mitochondrial introns aI1 and/or aI2 are needed for the in vivo deletion of intervening sequences.
abstract: Some pet- (or mit-) mutations impeding the splicing of one or several intron(s) of the yeast mitochondrial pre-mRNA(s) are suppressed in vivo by the DNA deletion of these introns. We have genetically demonstrated that introns aI1 and/or aI2 of the cytochrome c oxidase subunit 1 gene are necessary for this deletion process. The facts that adjacent introns are simultaneously deleted and that, in the pet- (or mit-) mutants which easily revert by intron deletion, the splicing of the introns they affect is only partially blocked, suggest that the intron encoded proteins aI1 and/or aI2 could intervene by means of their putative reverse transcriptase activity.
authors: Institut Curie, Section de Biologie, Centre Universitaire, Orsay, France.
authors: E
authors: E
authors: Levra-Juillet
authors: A
authors: A
authors: Boulet
authors: B
authors: B
authors: Séraphin
authors: M
authors: M
authors: Simon
authors: G
authors: G
authors: Faye
mesh_terms: D002872:Chromosome Deletion; D004271:DNA, Fungal / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D005838:Genotype; D007438:Introns; D008928:Mitochondria / Q000502:physiology*; D009154:Mutation; D012322:RNA Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012194:RNA-Directed DNA Polymerase / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism
chemical_list: D004271:DNA, Fungal; D012322:RNA Precursors; D012333:RNA, Messenger; D003576:Electron Transport Complex IV; D012194:RNA-Directed DNA Polymerase