title: Continuous bromodeoxyuridine labeling and bivariate ethidium bromide/Hoechst flow cytometry in cell kinetics.
abstract: Most techniques of flow cytometric cell cycle analysis are not capable of distinguishing the number of rounds of DNA synthesis that a cell has undergone since the start of an experiment. Continuous labeling with 5-bromodeoxyuridine (BrdUrd) offers such a potential. We illustrate here that the bivariate analysis of non-BrdUrd-quenched ethidium bromide vs. BrdUrd-quenched Hoechst 33258 fluorescence offers a high degree of resolution that enhances the analytical power of the technique, and that this approach can be applied to the analysis of a broad range of human and murine primary cells and established cell lines.
authors: Department of Human Genetics, University of Wuerzburg, Federal Republic of Germany.
authors: M
authors: M
authors: Poot
authors: H
authors: H
authors: Schmitt
authors: H
authors: H
authors: Seyschab
authors: J
authors: J
authors: Koehler
authors: U
authors: U
authors: Chen
authors: U
authors: U
authors: Kaempf
authors: M
authors: M
authors: Kubbies
authors: D
authors: D
authors: Schindler
authors: P S
authors: PS
authors: Rabinovitch
authors: H
authors: H
authors: Hoehn
mesh_terms: D006690:Bisbenzimidazole; D001973:Bromodeoxyuridine; D002453:Cell Cycle; D002478:Cells, Cultured; D004261:DNA Replication; D004996:Ethidium; D005347:Fibroblasts / Q000032:analysis* / Q000166:cytology; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans
chemical_list: D004996:Ethidium; D001973:Bromodeoxyuridine; D006690:Bisbenzimidazole