Cytokeratin filaments are present in golden hamster oocytes and early embryos.
Light and electron microscope methods were used to study cytokeratin expression in the recently ovulated oocytes, fertilized eggs and early embryos from the golden hamster. Two cytokeratin polypeptides (Mr 51,000 and 58,000) were detected in oocyte lysates by immunoblotting using a polyclonal antiserum to prekeratin. In the oocyte, cytokeratin occurred as patchy aggregates consisting of short anastomosing 10-nm filaments that formed tight meshworks distributed throughout the cytoplasm. After fertilization the aggregates appeared to merge, becoming larger and concentrated at the cortical region. Prominent immunofluorescent fibrils were seen interconnecting the aggregates. In the 2-, 4- and 8-cell embryos, networks of cytokeratin filaments extended throughout the cortical and perinuclear regions, while aggregates progressively disappeared.
Plancha
C E
CE
Institute of Histology and Embryology, Faculty of Medicine, University of Lisbon.
Carmo-Fonseca
M
M
David-Ferreira
J F
JF
D000818:Animals; D006224:Cricetinae; D003599:Cytoskeleton / Q000648:ultrastructure*; D004622:Embryo, Mammalian / Q000378:metabolism / Q000648:ultrastructure*; D005260:Female; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D007382:Intermediate Filaments / Q000378:metabolism / Q000648:ultrastructure*; D007633:Keratins / Q000378:metabolism*; D008647:Mesocricetus; D008854:Microscopy, Electron; D009865:Oocytes / Q000378:metabolism / Q000648:ultrastructure*
D007633:Keratins