The gene that encodes the human CD20 (B1) differentiation antigen is located on chromosome 11 near the t(11;14)(q13;q32) translocation site.
The human CD20 gene (B1) encodes a B lymphocyte-specific, cell-surface molecule that is involved in B cell activation and differentiation. We report that the CD20 gene is located on human chromosome 11 at position q12-q13. The location of CD20 was determined by in situ hybridization and was further confirmed by Southern blot analysis of DNA from rodent/human hybrids that contained only portions of human chromosome 11. This localization places the CD20 gene near the site of the t(11;14)(q13;q32) translocation that is found in a subgroup of B cell-lineage malignancies. The site of this translocation has been previously identified by DNA cloning and termed bcl-1. The CD20 gene was found to lie on the centromeric side of bcl-1 on chromosome 11 and to be separated from bcl-1 by at least 50 kb of DNA. These results raise the possibility that alterations in the expression of the CD20 gene may result after the t(11;14) chromosomal alteration.
Tedder
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Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, MA 02115.
Disteche
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Louie
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Adler
D A
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Croce
C M
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Schlossman
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Saito
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D000818:Animals; D018951:Antigens, CD20; D000944:Antigens, Differentiation, B-Lymphocyte / Q000235:genetics*; D001402:B-Lymphocytes / Q000032:analysis; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D008040:Genetic Linkage; D006801:Humans; D008297:Male; D051379:Mice; D011518:Proto-Oncogene Proteins / Q000235:genetics; D014178:Translocation, Genetic
D018951:Antigens, CD20; D000944:Antigens, Differentiation, B-Lymphocyte; D011518:Proto-Oncogene Proteins