Sialic acid-dependent adhesion of Mycoplasma pneumoniae to purified glycoproteins.
Several purified glycoproteins including laminin, fetuin, and human chorionic gonadotropin promote dose-dependent and saturable adhesion of Mycoplasma pneumoniae when adsorbed on plastic. Adhesion to the proteins is energy dependent as no attachment occurs in media without glucose. Adhesion to all of the proteins requires sialic acid, and only those proteins with alpha 2-3-linked sialic acid are active. The alpha-subunit of human chorionic gonadotropin also promotes attachment, suggesting that a simple biantennary asparagine-linked oligosaccharide is sufficient for binding. Soluble laminin, asparagine-linked sialyloligosaccharides from fetuin, and 3'-sialyllactose but not 6'-sialyllactose inhibit attachment of M. pneumoniae to laminin. M. pneumoniae also bind to sulfatide adsorbed on plastic. Dextran sulfate, which inhibits M. pneumoniae binding to sulfatide, does not inhibit attachment on laminin, and 3'-sialyllactose does not inhibit binding to sulfatide, suggesting that two distinct receptor specificities mediate binding to these two carbohydrate receptors. Both 3'-sialyllactose and dextran sulfate partially inhibit M. pneumoniae adhesion to a human colon adenocarcinoma cell line (WiDr) at concentrations that completely inhibit binding to laminin or sulfatide, respectively, and in combination they inhibit binding of M. pneumoniae to these cells by 90%. Thus, both receptor specificities contribute to M. pneumoniae adhesion to cultured human cells.
Roberts
D D
DD
Laboratory of Structural Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892.
Olson
L D
LD
Barile
M F
MF
Ginsburg
V
V
Krivan
H C
HC
D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects; D001667:Binding, Competitive; D002236:Carbohydrate Conformation; D006063:Chorionic Gonadotropin / Q000502:physiology; D006023:Glycoproteins / Q000502:physiology*; D006031:Glycosylation; D007785:Lactose / Q000031:analogs & derivatives / Q000502:physiology; D007797:Laminin / Q000502:physiology; D051379:Mice; D009177:Mycoplasma pneumoniae / Q000187:drug effects / Q000502:physiology*; D019158:N-Acetylneuraminic Acid; D009439:Neuraminidase; D009844:Oligosaccharides / Q000494:pharmacology; D012794:Sialic Acids / Q000502:physiology*; D000509:alpha-Fetoproteins / Q000502:physiology
D006063:Chorionic Gonadotropin; D006023:Glycoproteins; D007797:Laminin; D009844:Oligosaccharides; D012794:Sialic Acids; D000509:alpha-Fetoproteins; C000020:N-acetylneuraminoyllactose; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid; D007785:Lactose