title: Characterization of keratinocyte plasminogen activator inhibitors and demonstration of the prevention of pemphigus IgG-induced acantholysis by a purified plasminogen activator inhibitor.
abstract: To investigate the mechanisms by which cutaneous plasminogen activator (PA) may be regulated, we have tested cultured keratinocytes for the presence of PA inhibitors. Using biosynthetic labeling experiments with 35S-methionine in conjunction with specific antibody precipitation, we have shown that human keratinocytes in culture synthesized and secreted both PA inhibitor 1 and PA inhibitor 2. PA inhibitor 1 was present in conditioned media in the inactive form, but it could be detected with reverse phase autography. PA inhibitor 2 was detected by its ability to form complexes with 125I-uPA. Potential therapeutic relevance for cutaneous PA inhibitor 2 was suggested in skin organ culture experiments which demonstrated that purified PA inhibitor 2 from human placenta was able to prevent the acantholytic changes induced by pemphigus IgG.
authors: Department of Dermatology, University of Pennsylvania, Philadelphia 19104.
authors: K
authors: K
authors: Hashimoto
authors: T C
authors: TC
authors: Wun
authors: J
authors: J
authors: Baird
authors: G S
authors: GS
authors: Lazarus
authors: P J
authors: PJ
authors: Jensen
mesh_terms: D000051:Acantholysis / Q000276:immunology* / Q000517:prevention & control; D002478:Cells, Cultured; D046508:Culture Techniques; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis / Q000502:physiology*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D007633:Keratins; D010392:Pemphigus / Q000276:immunology*; D010960:Plasminogen Activators / Q000037:antagonists & inhibitors*; D015849:Plasminogen Inactivators; D012871:Skin Diseases / Q000276:immunology*; D014568:Urokinase-Type Plasminogen Activator / Q000037:antagonists & inhibitors*
chemical_list: D006023:Glycoproteins; D007074:Immunoglobulin G; D015849:Plasminogen Inactivators; D007633:Keratins; D010960:Plasminogen Activators; D014568:Urokinase-Type Plasminogen Activator