Free radicals induce ischemia-reperfusion injury and histamine release in the isolated guinea pig heart.
Free radicals are produced by perfusion of isolated guinea pig heart with FeCl3/ADP (10 microM/100 microM) and/or occlusion and opening of the left anterior descending coronary artery. Cardiac histopathology was correlated with histamine and lactate dehydrogenase release and with malondialdehyde production. A differential release of histamine and lactate dehydrogenase in the perfusate was detected, showing a preferential liberation of histamine in the reperfusion phase. The increase in lipid peroxidation product in left ventricular tissues after left coronary artery occlusion was maximal at the end of ischemia.
Masini
E
E
Department of Preclinical and Clinical Pharmacology, University of Florence, Italy.
Giannella
E
E
Palmerani
B
B
Pistelli
A
A
Gambassi
F
F
Mannaioni
P F
PF
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D002712:Chlorides; D005290:Ferric Compounds / Q000494:pharmacology; D005609:Free Radicals; D006168:Guinea Pigs; D006636:Histamine Release; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D010100:Oxygen / Q000633:toxicity*; D015427:Reperfusion Injury / Q000378:metabolism*
D002712:Chlorides; D005290:Ferric Compounds; D005609:Free Radicals; D000244:Adenosine Diphosphate; D007770:L-Lactate Dehydrogenase; D010100:Oxygen; C024555:ferric chloride