Use of monoclonal antibodies to serotype-specific antigens of Haemophilus pleuropneumoniae serotype 2 in passive immunization.
The prophylactic value of mouse monoclonal antibodies to the pig pathogen Haemophilus pleuropneumoniae was studied. Approximately 250 mg of purified mouse monoclonal antibody specific to capsular antigens of H pleuropneumoniae serotype 2 was given IV to five 9-week-old pigs. Five additional pigs from the same litter served as controls. On the following day, all pigs were given a lethal dose (5 x 10(9)) of H pleuropneumoniae serotype 2 into the trachea. Four controls and 1 pig that was given antibodies died within 24 hours. The surviving 5 pigs developed typical signs of pleuropneumonia. After 6 days, the pigs were euthanatized and their respiratory tracts were examined for pathologic changes. All 5 pigs had pathologic changes, but they were less severe in the 4 pigs that had been given antibodies, compared with those in the control pig.
Korvuo
A
A
Department of Anatomy and Embryology, College of Veterinary Medicine, Helsinki, Finland.
Lindberg
L A
LA
Tuomi
J
J
Schröder
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D000939:Epitopes / Q000276:immunology; D006190:Haemophilus / Q000276:immunology*; D006192:Haemophilus Infections / Q000517:prevention & control / Q000662:veterinary; D007116:Immunization, Passive / Q000379:methods / Q000662:veterinary*; D013552:Swine / Q000276:immunology*; D013553:Swine Diseases / Q000517:prevention & control
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes