Muscarinic receptor-mediated increase in cAMP levels in SK-N-SH human neuroblastoma cells.
Stimulation of muscarinic cholinergic receptors in SK-N-SH human neuroblastoma cells resulted in a 1.5-4 fold increase in intracellular cAMP levels. This unusual response was sensitive to atropine and pirenzepine but insensitive to pertussis toxin. It was observable regardless of whether basal, PGE1- or forskolin-stimulated cAMP levels were measured. The half-maximal concentration for carbachol-stimulation of cAMP levels (6 microM) was similar to that for the previously determined carbachol-induced stimulation of phosphoinositide turnover in these cells, suggesting that the former is mediated by the latter. These data indicate that cross-talk between the phosphoinositide turnover system and the adenylate cyclase system results in increased cAMP levels in SK-N-SH cells in response to muscarinic receptor stimulation.
Baumgold
J
J
Membrane Biochemistry Section, National Institute of Neurological and Communicative Disorders and Stroke, Bethesda, MD 20892.
Fishman
P H
PH
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D037361:Adenylate Cyclase Toxin; D002217:Carbachol / Q000494:pharmacology*; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D009447:Neuroblastoma; D010095:Oxotremorine / Q000494:pharmacology*; D037342:Pertussis Toxin; D011976:Receptors, Muscarinic / Q000502:physiology*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D037361:Adenylate Cyclase Toxin; D011976:Receptors, Muscarinic; D010566:Virulence Factors, Bordetella; D005576:Colforsin; D010095:Oxotremorine; D002217:Carbachol; D000242:Cyclic AMP; D037342:Pertussis Toxin; D015056:1-Methyl-3-isobutylxanthine