Recognition of oligonucleotide-encoded T cell epitopes introduced into a gene unrelated to the original antigen.
We have previously demonstrated that H-2Kd-restricted CTL specific for HLA-CW3 or HLA-A24 can recognize synthetic peptides corresponding to residues 170-182 of the HLA molecules. Synthetic oligonucleotides encoding region 170-182 of CW3 or A24 were inserted into the influenza nucleoprotein (NP) gene. We demonstrate herein that P815 (H-2d) cells transfected with the NP-oligo recombinant genes are specifically lysed by HLA-specific Kd-restricted CTL clones. Our results imply that there must be a high degree of flexibility for the expression of T cell epitopes in different molecular contexts.
Chimini
G
G
Centre d'Immunologie INSERM, Centre National de Recherche Scientifique de Marseille-Luminy, Marseille, France.
Pala
P
P
Sire
J
J
Jordan
B R
BR
Maryanski
J L
JL
D000818:Animals; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D006680:HLA Antigens / Q000235:genetics / Q000276:immunology*; D006801:Humans; D051379:Mice; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins / Q000235:genetics / Q000276:immunology*; D009841:Oligonucleotides / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014162:Transfection; D014758:Viral Core Proteins; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*
D000939:Epitopes; D006680:HLA Antigens; C048842:NP protein, Influenza A virus; D019590:Nucleocapsid Proteins; D009698:Nucleoproteins; D009841:Oligonucleotides; D010446:Peptide Fragments; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D014758:Viral Core Proteins; D014764:Viral Proteins