Antimitochondrial autoantibodies in primary biliary cirrhosis recognize cross-reactive epitope(s) on protein X and dihydrolipoamide acetyltransferase of pyruvate dehydrogenase complex.
Antimitochondrial autoantibodies are characteristically present in sera of patients with primary biliary cirrhosis. The antimitochondrial autoantibodies recognize four major antigens from beef heart mitochondria at relative molecular weights of 74, 56, 52 and 48 kD. In the present study, we report that the 56 kD antigen is the protein X of pyruvate dehydrogenase complex and that it possesses cross-reactive antimitochondrial autoantibody epitope(s) with the 74 kD antigen, the acetyltransferase (E2) of the pyruvate dehydrogenase complex. This was demonstrated by comparing the specificities of primary biliary cirrhosis sera with a protein X-specific rabbit antiserum and by absorbing primary biliary cirrhosis sera with recombinant pyruvate dehydrogenase-E2 fusion protein. In the two-dimensional gel analysis, primary biliary cirrhosis sera and protein X-specific rabbit antiserum reacted to the same two isoelectric point polypeptides at 56 kD molecular weight. The absorption of primary biliary cirrhosis sera with the human recombinant pyruvate dehydrogenase-E2 removed reactivity toward both the 74 and 56 kD antigens. Furthermore, analysis of 82 antimitochondrial autoantibody-positive primary biliary cirrhosis sera by immunoblotting did not reveal any sera which reacted solely against either the 74 or 56 kD antigen. Finally, primary biliary cirrhosis sera recognized protein X from human, bovine and porcine sources but not protein X from rat or mouse origin. The identification of protein X as another major target of the autoimmune response in primary biliary cirrhosis suggests that the pyruvate dehydrogenase complex may have a central role in the induction of this enigmatic disease.
Surh
C D
CD
Department of Internal Medicine, School of Medicine, University of California, Davis 95616.
Roche
T E
TE
Danner
D J
DJ
Ansari
A
A
Coppel
R L
RL
Prindiville
T
T
Dickson
E R
ER
Gershwin
M E
ME
D000123:Acetyltransferases / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D001323:Autoantibodies / Q000276:immunology*; D015153:Blotting, Western; D003429:Cross Reactions; D051047:Dihydrolipoyllysine-Residue Acetyltransferase; D015180:Electrophoresis, Gel, Two-Dimensional; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D008105:Liver Cirrhosis, Biliary / Q000276:immunology*; D008929:Mitochondria, Heart / Q000276:immunology*; D010455:Peptides / Q000276:immunology*; D011768:Pyruvate Dehydrogenase Complex / Q000276:immunology*; D013045:Species Specificity
D001323:Autoantibodies; D000939:Epitopes; C045867:PDHX protein, human; D010455:Peptides; D011768:Pyruvate Dehydrogenase Complex; C489061:pyruvate dehydrogenase complex component X, Bos taurus; D000123:Acetyltransferases; D051047:Dihydrolipoyllysine-Residue Acetyltransferase