Degradation of endogenous proteins and internalized asialofetuin in primary cultured hepatocytes of rats.
1. The effects of various metabolic and enzyme inhibitors on the degradation of endogenous proteins and internalized asialofetuin were examined in the primary cultured hepatocytes of rats. 2. The results showed the important physiological role of bestatin-sensitive aminopeptidases in the degradation of endogenous and internalized proteins as well as in the degradation of both long- and short-lived proteins.
Kato
H
H
Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo, Japan.
Takahashi
S
S
Takenaka
A
A
Funabiki
R
R
Noguchi
T
T
Naito
H
H
D000596:Amino Acids / Q000302:isolation & purification; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001212:Asialoglycoproteins; D002478:Cells, Cultured; D060748:Fetuins; D005934:Glucagon / Q000502:physiology; D007328:Insulin / Q000494:pharmacology; D007930:Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D007976:Leupeptins / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D010455:Peptides / Q000302:isolation & purification / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012588:Scintillation Counting; D000509:alpha-Fetoproteins / Q000378:metabolism*
D000596:Amino Acids; D000900:Anti-Bacterial Agents; D001212:Asialoglycoproteins; D060748:Fetuins; D007328:Insulin; D007976:Leupeptins; D010455:Peptides; D011506:Proteins; D000509:alpha-Fetoproteins; C017029:asialofetuin; D005934:Glucagon; D007930:Leucine; C012211:ubenimex