title: A conditional high-copy-number plasmid derivative of pBR322.
abstract: A spontaneous high-copy-number plasmid derivative of plasmid pBR322 was isolated. This plasmid bears two point mutations adjacent to the unpaired region in the stem of loop II of RNA I and expresses its high-copy-number phenotype only when the harboring cells grow on solid support (L-agar).
authors: Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
authors: I
authors: I
authors: Ivanov
authors: L
authors: L
authors: Yavashev
authors: L
authors: L
authors: Gigova
authors: K
authors: K
authors: Alexciev
authors: C
authors: C
authors: Christov
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis*; D009154:Mutation; D009690:Nucleic Acid Conformation; D010641:Phenotype; D010957:Plasmids; D012329:RNA, Bacterial; D012093:Replicon
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial