[Effects of interferon on the cell cycle of human hepatoma HLF cells analyzed by flow cytometry].
Growth inhibition by interferon (IFN) was investigated in human hepatoma HLF cells by use of flow cytometry to study the cell cycle. INF-alpha or -beta inhibited growth more than IFN-gamma. Use of either IFN-alpha or -beta and IFN-gamma at the same time inhibited growth more than with any one kind of IFN, but use of IFN-alpha and -beta together did not cause much inhibition. IFN inhibited growth by causing cells to accumulate in the S phase instead of moving on to the G2 phase. Accumulation in the S phase was less with IFN-gamma than with -alpha or -beta. It increased with the combination of IFN-alpha or -beta with IFN-gamma, but not with the combination of IFN-alpha and -beta.
Takeda
T
T
Nishiguchi
S
S
Kuroki
T
T
Ichiba
K
K
Shimizu
Y
Y
Nakajima
S
S
Shiomi
S
S
Kobayashi
K
K
D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002453:Cell Cycle; D003864:Depression, Chemical; D005434:Flow Cytometry; D006801:Humans; D007372:Interferons / Q000008:administration & dosage / Q000494:pharmacology*; D008113:Liver Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
D007372:Interferons