Acceleration of anterograde axonal transport in cat sciatic nerve by diisopropyl phosphorofluoridate.
The effect of a neurotoxic dose of 5.0 mg/kg, s.c. diisopropyl phosphorofluoridate (DFP) on anterograde transport of 35S-methionine labeled proteins in cat peripheral nerve was studied. Seven days after dosing, after 24 h of flow there was 48% less radioactivity in the distal portion of the nerve. A lesser effect was also found at 4 and 14 days after dosing. After 10 or 14 h of flow, the height of the crest was unchanged, but the distance of the crest from the ganglia was greater in the DFP-treated animals. These experiments indicate that DFP treatment accelerates fast anterograde transport.
Carrington
C D
CD
Department of Pharmacology, Duke University Medical Center, Durham, NC 27710.
Lapadula
D M
DM
Abou-Donia
M B
MB
D000818:Animals; D001370:Axonal Transport / Q000187:drug effects*; D002415:Cats; D005260:Female; D007531:Isoflurophate / Q000494:pharmacology* / Q000633:toxicity; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D012584:Sciatic Nerve / Q000378:metabolism*
D009419:Nerve Tissue Proteins; D007531:Isoflurophate