Substance P stimulates translocation of protein kinase C in brain microvessels.
The action of substance P on protein kinase C in cerebral microvessels, isolated from bovine, was investigated. We found that in untreated microvessels 85% of the total activity was localized to the cytosolic fraction. Substance P caused a shift of activity to the membrane fraction, accompanied by a loss of activity in the cytosolic fraction. This effect resulted in dose-dependence and it was evident after 5 min treatment. These data suggest that substance P may be involved in the regulation of processes underlying protein phosphorylation in the blood-brain barrier.
Catalán
R E
RE
Departamento de Biología Molecular, Universidad Autónoma de Madrid, Spain.
Martínez
A M
AM
Aragonés
M D
MD
Fernández
I
I
D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000201:enzymology; D002560:Cerebrovascular Circulation; D003600:Cytosol / Q000201:enzymology; D007700:Kinetics; D008833:Microcirculation / Q000201:enzymology*; D011493:Protein Kinase C / Q000378:metabolism*; D013373:Substance P / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D013373:Substance P; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate