An increase in intracellular pH is a general response of promonocytic cells to differentiating agents.
Intracellular pH (pHi) was measured in HL60 and U937 cells before and after differentiation into monocyte-macrophage like cells. 12-O-Tetradecanoyl phorbol-13-acetate (PMA), butyrate, interferon, retinoic acid and 1,25-dihydroxyvitamin D3 all increased pHi. The increases elicited were rapid with PMA, much slower with retinoic acid and interferon and still slower with 1,25-dihydroxyvitamin D3. Increases in pHi are due to an activation of the Na+/H+ exchange system. High pHi values are unlikely to serve as an early intracellular signal for initiating monocytic differentiation.
Ladoux
A
A
INSERM U 204, Hopital Saint Louis, Paris, France.
Damais
C
C
Krawice
I
I
Abita
J P
JP
Frelin
C
C
D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002117:Calcitriol / Q000494:pharmacology; D002454:Cell Differentiation; D002460:Cell Line; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007372:Interferons / Q000494:pharmacology; D007700:Kinetics; D011994:Recombinant Proteins / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology
D002087:Butyrates; D011994:Recombinant Proteins; D020148:Butyric Acid; D014212:Tretinoin; D007372:Interferons; D002117:Calcitriol; D013755:Tetradecanoylphorbol Acetate