Autoradiographic study of RNA synthesis in adrenal cells of the young rat in primary culture.
Primary cultures of young rat adrenal cells were used in a radioautographic study of the effect of ACTH on RNA synthesis. Control and ACTH-stimulated cultures were incubated with 5.6 [3H]-uridine for 30 min and chased for 15 and 30 min. Labelling over nucleolar and extranucleolar areas was always lower in the ACTH-stimulated animals at all experimental times. At 30 min chase-time a relatively high labelling of nuclei in both experimental conditions suggest the slow metabolism of RNA in adrenal. The results confirm the inhibitor effect of adrenocorticotrophin on [3H]-uridine incorporation.
Magalhães
M C
MC
Institute of Histology and Embryology, Faculty of Medicine, University of Porto, Portugal.
Bonito-Vitor
A
A
Magalhães
M M
MM
D000311:Adrenal Glands / Q000166:cytology* / Q000378:metabolism; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000818:Animals; D001345:Autoradiography; D002478:Cells, Cultured; D003593:Cytoplasm / Q000032:analysis; D008854:Microscopy, Electron; D012313:RNA / Q000096:biosynthesis*; D051381:Rats
D012313:RNA; D000324:Adrenocorticotropic Hormone