title: Dopamine accumulation in large unilamellar vesicle systems induced by transmembrane ion gradients.
abstract: Transmembrane movement of dopamine in response to K+ or H+ ion gradients has been investigated. It is shown that dopamine can accumulate rapidly into large unilamellar vesicles (LUVs) composed of egg phosphatidylcholine exhibiting either a K+ diffusion potential (delta psi; negative inside) or a pH gradient (inside acidic). This can result in entrapped dopamine concentrations of 30-40 mM and inside-outside concentration gradients of nearly 300-fold. The transmembrane dopamine gradients formed in LUV systems exhibiting delta pH (inside acidic) indicate that the transport process can be dictated by movement of the neutral form of dopamine which redistributes according to a simple Henderson-Hasselbach equilibrium. The mechanism of dopamine transport in response to a valinomycin-induced K+ potential is more complex. Although generation of a K+ diffusion potential results in acidification of the vesicle interior, the magnitude of the induced delta pH (approx. 1 pH unit) is insufficient to account for the dopamine concentration gradient achieved (greater than 200-fold). Further, data presented here suggest that higher uptake levels of dopamine can be achieved when certain anions (ATP and citrate) are entrapped within the LUV system. These anions may complex with the protonated form of dopamine creating a non-equilibrium trapping phenomena resulting in interior concentrations of dopamine in excess of that predicted by a simple Henderson-Hasselbach equilibrium.
authors: Biochemistry Department, University of British Columbia, Vancouver, Canada.
authors: M B
authors: MB
authors: Bally
authors: L D
authors: LD
authors: Mayer
authors: H
authors: H
authors: Loughrey
authors: T
authors: T
authors: Redelmeier
authors: T D
authors: TD
authors: Madden
authors: K
authors: K
authors: Wong
authors: P R
authors: PR
authors: Harrigan
authors: M J
authors: MJ
authors: Hope
authors: P R
authors: PR
authors: Cullis
mesh_terms: D004298:Dopamine / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007473:Ion Channels / Q000378:metabolism*; D008051:Lipid Bilayers; D008564:Membrane Potentials; D010713:Phosphatidylcholines; D011188:Potassium / Q000378:metabolism
chemical_list: D007473:Ion Channels; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D011188:Potassium; D004298:Dopamine