Altered gelatinolytic activity by keratoconus corneal cells.
Keratoconus involves thinning and central protuberance of the cornea, scarring and significantly decreased vision. It is one of the major causes of corneal transplantation in this country, but the etiology of this disorder is unclear. In the present study stromal keratocytes were isolated and cultured from normal and keratoconus human corneas. Consistent with the phenotype of cornea thinning, we observed an increased gelatinolytic activity in keratoconus cultures. Characterization of enzyme properties in these cells suggested that gelatinase (type IV collagenase) was responsible for the majority of proteolytic activity found in this system. This elevated gelatinolytic activity was present in spite of lower amounts of total protein being produced by the keratoconus cultures.
Kenney
M C
MC
Department of Ophthalmology Research, Cedars-Sinai Medical Center, Los Angeles, CA 90048.
Chwa
M
M
Escobar
M
M
Brown
D
D
D002478:Cells, Cultured; D003315:Cornea / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional; D018093:Gelatinases; D007633:Keratins / Q000378:metabolism*; D007640:Keratoconus / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D010434:Pepsin A / Q000378:metabolism; D011486:Protein C / Q000378:metabolism; D013379:Substrate Specificity
D011486:Protein C; D007633:Keratins; D010434:Pepsin A; D018093:Gelatinases