A comparison of reverse transcriptase and antigen capture assays for the detection of HIV.
HIV antigen capture assay (ELISA) and reverse transcriptase assay were compared for the detection of HIV. Purified virus and the supernatant of infected cells were used for this study. Antigen capture assay was found to be at least one hundred times more sensitive than the RT assay, and for a large number of samples easier to perform. This assay would be useful for clinical and laboratory diagnosis.
Lee
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Lindsley F. Kimball Research Institute, New York Blood Center, New York 10021.
D000163:Acquired Immunodeficiency Syndrome / Q000175:diagnosis; D000956:Antigens, Viral / Q000032:analysis*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D006678:HIV / Q000201:enzymology / Q000276:immunology / Q000302:isolation & purification*; D015488:HIV Antigens; D006801:Humans; D012194:RNA-Directed DNA Polymerase / Q000032:analysis*
D000956:Antigens, Viral; D015488:HIV Antigens; D012194:RNA-Directed DNA Polymerase