[Assessment of the intensity of lymphocyte blast transformation in the crypts of the palatal tonsils by using DNA cytophotometry].
Cell fractions isolated from lacunae of palatine tonsils with different functional activities were taken from 40 children 6-15 years of age. The cell fractions were examined using vital phase-contrast microscopy, light microscopy of Pappenheim-stained smears and the Feulgen cytophotometry. Lymphocytes with signs of immunological involvement were shown to constitute 95 per cent of the whole cell lacunar population of the active functional tonsils. 35 per cent of lymphocytes, being in different stages of blast transformation, were found. The most intense blast transformation was revealed in patients with tonsil inflammation. 5-13 per cent of lacunar nuclei with DNA content above the diploid level were found in these patients. Some multicellular islets accumulating small lymphocytes, lymphocytes at different stages of blast transformation, and macrophages were revealed to imitate the cooperation of immunocompetent cells. The data obtained can be helpful in the practical surgery of palatine tonsils.
Bogdanova
M S
MS
Popov
E L
EL
D000293:Adolescent; D002452:Cell Count; D002467:Cell Nucleus / Q000032:analysis; D002648:Child; D003592:Cytophotometry; D004247:DNA / Q000032:analysis*; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000032:analysis / Q000166:cytology; D008858:Microscopy, Phase-Contrast; D014066:Palatine Tonsil / Q000032:analysis / Q000166:cytology*; D013194:Staining and Labeling / Q000379:methods; D014069:Tonsillitis / Q000378:metabolism / Q000473:pathology
D004247:DNA
Otsenka intensivnosti blastnoĭ transformatsii limfotsitov v kriptakh nebnykh mindalin s pomoshch'iu tsitofotometrii DNK.