Immune response to cholera toxin epitope inserted in Salmonella flagellin.
Bacterial flagella are potent immunogens and aromatic-dependent (aro) Salmonella as live vaccines evoke humoral and cellular immune responses. Such strains expressing epitopes of protective antigens as inserts in flagellin would provide a novel way to vaccinate against diseases caused by unrelated pathogens. A synthetic oligonucleotide specifying an epitope of cholera toxin subunit B was inserted in a Salmonella flagellin gene. The chimeric flagellin functioned normally and the epitope was expressed at the flagellar surface. Parenteral administration to mice of an aro A flagellin-negative strain of S. dublin expressing the chimeric flagellin gene evoked antibody to cholera toxin.
Newton
S M
SM
Department of Microbiology and Immunology, Stanford University School of Medicine, CA 94305.
Jacob
C O
CO
Stocker
B A
BA
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000276:immunology*; D002772:Cholera Toxin / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D005408:Flagellin / Q000235:genetics / Q000276:immunology*; D005822:Genetic Vectors; D051379:Mice; D008810:Mice, Inbred C57BL; D015345:Oligonucleotide Probes; D010957:Plasmids; D012475:Salmonella / Q000235:genetics / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D000939:Epitopes; D015345:Oligonucleotide Probes; D014614:Vaccines, Synthetic; D005408:Flagellin; D002772:Cholera Toxin