Cell-specific hypomethylation of the pepsinogen gene in pepsinogen-producing cells.
The pepsinogen gene is hypomethylated in the stomach, in which it is expressed. For demonstration that this hypomethylation of the pepsinogen gene in the stomach reflects pepsinogen-producing cells, we analyzed fractions of dispersed mucosal cells with various contents of pepsinogen-producing cells prepared from guinea pig stomach by centrifugal elutriation. mRNA expression and the extent of hypomethylation of the pepsinogen gene in each fraction was closely correlated with the content of pepsinogen-producing cells. These results suggested hypomethylation of the pepsinogen gene in pepsinogen-producing cells and differential pepsinogen gene methylation in cell subpopulations in the stomach.
Ichinose
M
M
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.
Miki
K
K
Tatematsu
M
M
Mizuno
T
T
Mutai
M
M
Furihata
C
C
Ichihara
Y
Y
Ishihara
T
T
Tanji
M
M
Oka
H
H
D000818:Animals; D002498:Centrifugation; D004247:DNA / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism; D006168:Guinea Pigs; D008745:Methylation; D009693:Nucleic Acid Hybridization; D010435:Pepsinogens / Q000235:genetics*; D012313:RNA / Q000032:analysis
D010435:Pepsinogens; D012313:RNA; D004247:DNA