Modifications of the effect of bleomycin in the somatic mutation and recombination test in Drosophila melanogaster.
Exposure to oxygen has been implicated as an important mechanism of mutations, cancer and aging. Most data supporting this notion have been obtained in vitro, but the elaborate defense systems against oxygen stress in aerobic organisms make it difficult to extrapolate in vitro data to in vivo conditions. In the present investigation the somatic mutation and recombination test (SMART) in Drosophila with the wing spot system (Graf et al., 1984) has been used as an in vivo system to study the effect of oxygen radicals generated by bleomycin (BLM). BLM causes a dose-related increase of wing spots and this effect drastically increases by increasing oxygen in the atmosphere to 70%. Data from treatment of larvae of different ages, as well as post-treatment with oxygen, indicate that BLM can persist, presumably intercalated in DNA, and subsequently be activated by oxygen to generate free radicals. By the use of inversion heterozygosity, which eliminates somatic recombination, it was shown that the majority of wing spots induced by BLM emanate from somatic recombination. A small number of flies deviated from the rest by an abnormally high frequency of BLM-induced wing spots. Preliminary results from a selection of such flies indicate that this extreme response to BLM is genetically determined. Treatment with BLM was also combined with agents known to interfere with the defense mechanisms against radicals or function as radical scavengers. Only ascorbic acid cotreatment had a modifying effect on BLM mutagenicity. The other agents did not alter or at most had a marginal effect on BLM mutagenicity. These data indicate that the defense mechanisms do not constitute a limiting factor in this case. BLM intercalates between DNA bases, presumably giving little time and opportunity for modifying agents to react with radicals generated in direct contact with the gene targets. No effect of BLM was observed on male germ cells by measuring loss and non-disjunction of ring-X/Y, neither in air nor in a 70% oxygen atmosphere.
Cederberg
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H
Department of Genetic and Cellular Toxicology, University of Stockholm, Sweden.
Ramel
C
C
D000818:Animals; D001386:Azides / Q000494:pharmacology; D001761:Bleomycin / Q000633:toxicity*; D019328:Buthionine Sulfoximine; D002083:Butylated Hydroxyanisole / Q000494:pharmacology; D002374:Catalase / Q000037:antagonists & inhibitors; D002869:Chromosome Aberrations; D004331:Drosophila melanogaster / Q000235:genetics*; D004790:Enzyme Induction; D005260:Female; D005609:Free Radicals; D005978:Glutathione / Q000037:antagonists & inhibitors; D005982:Glutathione Transferase / Q000096:biosynthesis; D008297:Male; D008717:Methionine Sulfoximine / Q000031:analogs & derivatives / Q000494:pharmacology; D009152:Mutagenicity Tests; D009154:Mutation; D010100:Oxygen / Q000494:pharmacology; D011995:Recombination, Genetic; D019810:Sodium Azide; D013094:Spermatozoa / Q000187:drug effects; D013482:Superoxide Dismutase / Q000037:antagonists & inhibitors; D013893:Thiram / Q000494:pharmacology
D001386:Azides; D005609:Free Radicals; D013893:Thiram; D001761:Bleomycin; D008717:Methionine Sulfoximine; D002083:Butylated Hydroxyanisole; D019328:Buthionine Sulfoximine; D019810:Sodium Azide; D002374:Catalase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D005978:Glutathione; D010100:Oxygen