Micronucleus formation in different lymphocyte subpopulations in peplomycin-treated and control cultures.
Spontaneous micronucleus formation and micronucleus induction by peplomycin in B and T lymphocytes was studied by a recently developed MAC (Morphology, Antibody, Chromosomes) method allowing the immunologic identification of different cell lineages. Blood samples from 3 healthy donors were cultured in the presence of phytohaemagglutinin and pokeweed mitogen. An increased frequency of micronuclei was observed in peplomycin cultures compared with controls. B cells were found to be more sensitive to peplomycin induction than were T lymphocytes. In control cultures, pokeweed mitogen yielded a higher frequency of micronuclei than did phytohaemagglutinin. In both pokeweed- and phytohaemagglutinin-stimulated cultures, B cells showed a higher frequency of micronuclei than did T cells. The relative proportion of mitotic B cells was equal in pokeweed and phytohaemagglutinin cultures. In peplomycin cultures, the proportion of B cells decreased as compared with control cultures.
Slavutsky
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Academica Nacional de Medicina de Buenos Aires, Instituto de Investigaciones Hematológicas, Argentina.
Knuutila
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D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D001761:Bleomycin / Q000494:pharmacology*; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure*; D008297:Male; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects / Q000648:ultrastructure*; D015162:Micronucleus Tests; D008938:Mitosis / Q000187:drug effects; D017663:Peplomycin; D012016:Reference Values
D015703:Antigens, CD; D001761:Bleomycin; D017663:Peplomycin