In vitro proliferation of human large granular lymphocytes with v-raf/v-myc recombinant retrovirus.
The effect of infection with a retrovirus carrying v-raf/v-myc oncogenes (J2 virus) on the in vitro proliferation of human large granular lymphocytes (LGL) was investigated. LGL infected with J2 virus (J2LGL), unlike uninfected cells, grew with a proliferation peak eight days after infection. Such cells retained the morphology and functional properties typical of LGL. Furthermore, 5% of J2LGL produced virus the day after infection, whereas non-virus production was detectable five days later. These data indicate that J2 virus provides a transient mitogenic signal for LGL.
Peppoloni
S
S
Biological Response Modifiers Program, NCI-FCRF, Frederick, Maryland.
Blasi
E
E
Ortaldo
J R
JR
Rapp
U R
UR
Riccardi
C
C
Varesio
L
L
D002455:Cell Division; D002478:Cells, Cultured; D004274:DNA, Recombinant; D006801:Humans; D007372:Interferons / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000166:cytology / Q000382:microbiology / Q000502:physiology*; D016274:Oncogene Protein p55(v-myc); D027384:Oncogene Proteins v-raf; D009857:Oncogenes; D012190:Retroviridae / Q000235:genetics* / Q000502:physiology; D012191:Retroviridae Proteins / Q000235:genetics*
D004274:DNA, Recombinant; D007376:Interleukin-2; D016274:Oncogene Protein p55(v-myc); D012191:Retroviridae Proteins; D007372:Interferons; D027384:Oncogene Proteins v-raf