Bsp 12861 restriction fragment length polymorphism detects Ag(c/g) locus of human apolipoprotein B in all 17 persons studied.
Southern blot analysis of the genomic DNA from 17 individuals showed perfect correspondence between a Bsp 12861 restriction fragment length polymorphism (RFLP) and the Ag(c/g) locus on human apolipoprotein (apo) B. The RFLP polymorphism is caused by a C--------T transition at nucleotide 421 on the cDNA, resulting in a threonine-to-isoleucine conversion. Thus, three of the five Ag sites have now been tentatively located on the 4536 residue apo B peptide at amino acyl residues 71: Ag(c/g), 591 (Ag(a1/d), and 4154 Ag(t/z).
Ma
Y H
YH
Department of Chemistry, University of California, Los Angeles.
Wang
X B
XB
Bütler
R
R
Schumaker
V N
VN
D001055:Apolipoproteins B / Q000235:genetics* / Q000276:immunology; D002874:Chromosome Mapping; D000939:Epitopes / Q000235:genetics*; D005838:Genotype; D006801:Humans; D010641:Phenotype; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D001055:Apolipoproteins B; D000939:Epitopes