Molecular cloning of human myelin-associated glycoprotein.
The nucleotide sequence for human myelin-associated glycoprotein (MAG) and its deduced amino acid sequence, obtained by analysis of two overlapping cDNA clones isolated from a human brain cDNA library, is presented and compared to that reported for rat MAG. The sequence provides an open reading frame of 1,878 nucleotides encoding a peptide of 626 amino acids with a calculated molecular weight of 69.1 kD. It is 89% homologous in nucleotide sequence to the large isoform of rat MAG, with 95% homology in the amino acid sequence. It contains 9 potential glycosylation sites, one more than in rat, and shares other key features with rat MAG, including 5 immunoglobulin-like regions of internal homology, an RGD sequence, and potential phosphorylation sites. Its structure appears to be highly conserved in evolution, possibly suggesting a close interdependence between its structure and function. The human gene is located on the proximal long arm of chromosome 19 (19q12----q13.2).
Spagnol
G
G
Department of Neurology, College of Physician and Surgeons, Columbia Presbyterian Medical Center, New York, NY 10032.
Williams
M
M
Srinivasan
J
J
Golier
J
J
Bauer
D
D
Lebo
R V
RV
Latov
N
N
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009185:Myelin Proteins / Q000378:metabolism*; D018992:Myelin-Associated Glycoprotein
D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D004247:DNA