title: Structure and associated DNA-helicase activity of a general transcription initiation factor that binds to RNA polymerase II.
abstract: RAP30/74 is a heteromeric general transcription initiation factor which binds to RNA polymerase II. Here we report that preparations of RAP30/74 contain an ATP-dependent DNA helicase whose probable function is to melt the DNA at transcriptional start sites. The sequence of the RAP30 subunit of RAP30/74 indicates that RAP30 may be distantly related to bacterial sigma factors.
authors: Department of Medical Genetics, University of Toronto, Ontario, Canada.
authors: M
authors: M
authors: Sopta
authors: Z F
authors: ZF
authors: Burton
authors: J
authors: J
authors: Greenblatt
mesh_terms: D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004265:DNA Helicases / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D006801:Humans; D007700:Kinetics; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D012319:RNA Polymerase II / Q000378:metabolism*; D012521:Sarcosine / Q000494:pharmacology / Q000031:analogs & derivatives; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D002352:Carrier Proteins; D046911:Macromolecular Substances; D010750:Phosphoproteins; C046282:RNA polymerase II-binding proteins; C025231:sarkosyl; D012319:RNA Polymerase II; D004265:DNA Helicases; D012521:Sarcosine