Elucidation of the structure of the Pasteurella haemolytica serotype T10 lipopolysaccharide O-antigen by n.m.r. spectroscopy.
The aqueous-phase lipopolysaccharide isolated from Pasteurella haemolytica serotype T10 cells by the phenol-water extraction method was found to be S-type lipopolysaccharide which possessed O-antigenic polysaccharide chains composed only of D-galactose residues. Structural analysis of the O-polysaccharide, using a combination of 1D and 2D 1H- and 13C-n.m.r. methods, led to the identification of the disaccharide repeating-unit as [----3)-alpha-D-Galp-(1----3)-beta-D-Galf-(1----]n. The serological cross-reactivity between P. haemolytica serotypes T4 and T10 can now be related to the structural similarity of the antigenic LPS O-polysaccharides.
Richards
J C
JC
Division of Biological Sciences, National Research Council of Canada, Ottawa, Ontario.
Leitch
R A
RA
D000942:Antigens, Bacterial / Q000276:immunology; D002236:Carbohydrate Conformation; D004187:Disaccharides; D008070:Lipopolysaccharides / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D019081:O Antigens; D010325:Pasteurella / Q000145:classification / Q000276:immunology*; D012703:Serotyping
D000942:Antigens, Bacterial; D004187:Disaccharides; D008070:Lipopolysaccharides; D019081:O Antigens