[A study on accumulation of cholesterol in rat prostate].
An experimental model of prostatic hypertrophy was created by freezing the urethra at the opening of the ventral prostate in rats and administering a cholesterol-rich diet to induce cholesterol accumulation. Four-week-old Fisher rats were divided into a control group, a cholesterol-rich diet group (by adding 1% cholesterol to the diet), a frozen group, and a frozen and cholesterol-rich diet group, and were reared for 5 months. The animals were killed, and the ventral prostate was removed, weighed, and the lipid was extracted. Cholesterol content was measured by an enzymatic method. A cholesterol-rich diet without freezing had no effects on the ventral prostate, and after freezing alone, the weight of the prostate was smaller than that of the control group. However, in the group cholesterol-rich diet after urethral freezing, the cholesterol content per mg of tissue increased. This indicates that cholesterol accumulation can be induced in the prostate by administering a cholesterol-rich diet after creating the proper secretory disturbance.
Tsugaya
M
M
Department of Urology, Nagoya City University School of Medicine.
Okamura
T
T
Sakagami
H
H
Hirao
N
N
Watase
H
H
Ohtaguro
K
K
Kato
J
J
D000818:Animals; D002791:Cholesterol, Dietary / Q000009:adverse effects / Q000378:metabolism*; D008297:Male; D011467:Prostate / Q000378:metabolism*; D011470:Prostatic Hyperplasia / Q000209:etiology / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344
D002791:Cholesterol, Dietary