Further characterization of the cation channel of a yeast vacuolar membrane in a planar lipid bilayer.
A voltage-dependent and Ca2(+)-activated cation channel recently found in the vacuolar membrane of the yeast Saccharomyces cerevisiae was incorporated into planar lipid bilayers and further characterized in macroscopic and single channel levels. Single channel conductances for various cations were in the order: NH4+ greater than K+ greater than Rb+ greater than Cs+ greater than Na+ greater than Li+, and were nearly consistent with the order of permeability ratio estimated from reversal potentials determined by macroscopic measurement. Up to 6 mM of Ca2+ added to the cis (cytoplasmic) side opened the channel, but higher concentrations closed the channel without affecting the single channel conductance. Ba2+ closed the channel without affecting the single channel conductance. Ba2+ closed the channel from the cis side. In addition to the above channel, a small cation-selective channel of about 40 pS was found.
Sato
M
M
Department of Biophysical Engineering, Faculty of Engineering Science, Osaka University, Japan.
Tanifuji
M
M
Kasai
M
M
D000838:Anions; D001464:Barium / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002412:Cations; D004553:Electric Conductivity; D007425:Intracellular Membranes / Q000378:metabolism; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D008051:Lipid Bilayers; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D014617:Vacuoles / Q000378:metabolism
D000838:Anions; D002412:Cations; D007473:Ion Channels; D008051:Lipid Bilayers; D001464:Barium; D002118:Calcium