Opsonization of normal myelin by anti-myelin antibodies and normal serum.
Fc receptor-dependent myelin phagocytosis has been proposed as a possible important effector mechanism in several immune-mediated demyelinating diseases. The present study was designed to determine whether myelin is opsonizable by anti-myelin antibodies. Thioglycolate-elicited mouse peritoneal macrophages were cultured with 125I-labelled bovine central myelin pretreated with normal or immune serum. Serum opsonic activity was determined by a kinetic study comparing macrophage uptake of opsonized and untreated 125I-myelin. Heat-stable and heat-labile myelin opsonins were detected in normal rabbit serum. Myelin was also opsonized by normal rabbit gamma globulin and by heat-inactivated normal mouse, human, and guinea pig serum. Increased opsonic activity was detected in rabbit anti-myelin antiserum and the gamma globulin fraction prepared from this serum, in anti-myelin basic protein and anti-galactocerebroside antiserum but not in anti-myelin-associated glycoprotein antiserum or in serum from rabbits injected with Freund's adjuvant alone. One out of three anti-sheep red blood cell antisera tested also showed increased myelin opsonic activity. It is concluded that anti-myelin antibodies can promote opsonic phagocytosis, and that normal serum and normal serum gamma globulin also opsonize myelin.
Goldenberg
P Z
PZ
Department of Neurosciences, UMDNJ-New Jersey Medical School, Newark.
Kwon
E E
EE
Benjamins
J A
JA
Whitaker
J N
JN
Quarles
R H
RH
Prineas
J W
JW
D000818:Animals; D000906:Antibodies / Q000502:physiology*; D001790:Blood Physiological Phenomena; D002417:Cattle; D004912:Erythrocytes / Q000276:immunology; D005699:Galactosylceramides / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D004676:Myelin Basic Protein / Q000276:immunology; D009185:Myelin Proteins / Q000276:immunology; D009186:Myelin Sheath / Q000276:immunology / Q000502:physiology*; D018992:Myelin-Associated Glycoprotein; D009895:Opsonin Proteins / Q000502:physiology*; D012016:Reference Values; D012756:Sheep / Q000097:blood
D000906:Antibodies; D005699:Galactosylceramides; D007106:Immune Sera; D004676:Myelin Basic Protein; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D009895:Opsonin Proteins; C002454:galactocerebroside