24-hour rabbit heart storage with UW solution. Effects of low-flow perfusion, colloid, and shelf storage.
A new technique for 24-hr cardiac preservation is described utilizing very low flow perfusion (microperfusion) with a cold flush solution. Rabbit hearts were arrested with UW solution and then perfused with the same solution through the aortic root at 0 degrees C at a rate of 3-6 ml/gm heart weight/24 hr. When tested on an ex vivo working heart model, the cardiac output (CO) was 28.72 +/- 7.69 ml/g/min compared with fresh UW flushed controls of 26.48 +/- 2.25 ml/g/min. Both oxygenated highflow perfusion with a more conventional perfusate and 24-hr ice storage with UW led to inferior results. Omission of the colloid, hydroxyethyl starch (HES), from the UW solution or prolonged shelf storage were also significantly detrimental. When a previously untested colloid, polyethylene glycol 20,000, was substituted for HES for microperfusion, excellent cardiac function was obtained. In fact, the mean CO of this group, 31.91 +/- 5.70, was significantly above that of fresh HES-UW unstored controls. The suggestion that the UW solution might be improved by this substitution warrants further study.
Wicomb
W N
WN
Pacific Presbyterian Medical Center, San Francisco, California 94120.
Collins
G M
GM
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D002314:Cardioplegic Solutions; D004355:Drug Stability; D005978:Glutathione; D006321:Heart; D006895:Hydroxyethyl Starch Derivatives / Q000494:pharmacology; D007036:Hypothermia, Induced; D007328:Insulin; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D010477:Perfusion / Q000379:methods; D011817:Rabbits; D011887:Raffinose; D012996:Solutions; D013997:Time Factors
D002314:Cardioplegic Solutions; D006895:Hydroxyethyl Starch Derivatives; D007328:Insulin; D019188:Organ Preservation Solutions; D012996:Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose