Cold storage preservation of pancreatic tissue prior to and after islet preparation in a dog autotransplantation model.
Utilizing an intrasplenic canine islet autotransplant model, the effects of cold storage preservation on pancreatic tissue prior to and after collagenase dispersion were examined. A control series, in which freshly retrieved and prepared tissue was transplanted, yielded a 75% success rate (6/8). In contrast, when the pancreas was stored in modified silica gel filtered plasma (SGF I) for 24 hr, no autotransplant was successful (0/6). However, when the islet tissue was prepared following pancreatectomy and then stored in a mannitol-containing modification of SGF (SGF III), autotransplantation was successful in 83% (5/6) after 24 hr of preservation and in 60% (3/5) after 48 hr of preservation. Similarly, the islet tissue was stored in a hyperkalemic hydroxyethyl starch solution (HES) and this was successful in 20% (1/5) after 24 hr of preservation and in 50% (1/2) after 48 hr of preservation. Cold storage preservation techniques for the pancreas prior to islet isolation need to be refined, but dispersed islet-enriched pancreatic tissue can be successfully maintained at 4 degrees C for up to 48 hr prior to transplantation in dogs using established pancreas preservation solutions.
Heise
J W
JW
Department of Surgery, University of Minnesota, Minneapolis 55455.
Casanova
D
D
Field
M J
MJ
Munn
S R
SR
Najarian
J S
JS
Sutherland
D E
DE
D000818:Animals; D001769:Blood; D003080:Cold Temperature; D004285:Dogs; D006440:Hemofiltration; D006895:Hydroxyethyl Starch Derivatives; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000378:metabolism; D016381:Islets of Langerhans Transplantation; D010179:Pancreas / Q000378:metabolism; D016035:Pancreas Transplantation; D011309:Preservation, Biological / Q000379:methods*; D058428:Silica Gel; D012822:Silicon Dioxide
D006895:Hydroxyethyl Starch Derivatives; D007328:Insulin; D058428:Silica Gel; D012822:Silicon Dioxide