title: Effect of secretin on pancreatic juice proteins in caerulein-induced acute pancreatitis in the rat.
abstract: Nine hours after the start of treatment with caerulein in rats, an increase in the weight of the pancreas and an increase in serum amylase levels were observed. Likewise, a significant increase in endogenous secretin occurred in rats with acute pancreatitis. A dramatic reduction in the secretion of total protein and amylase was also observed. A partial recovery of this latter effect was achieved after an infusion of high doses of secretin. Under our experimental conditions, the volume of secretion did not vary in caerulein-treated rats wtih respect to controls, either in resting conditions or under secretin stimulation, which indicates that the ductular cells were not significantly affected. Isoelectrofocusing (IEF) and crossed-immunoelectrophoresis (CIE) studies revealed important alterations in the proteins of the pancreatic juice of rats with caerulein-induced acute pancreatitis. Trypsinogen appeared to be particularly affected, showing an increase in the T2 acidic form with an IEP of 4.4 and a decrease in the basic form T3 with an IEP of 8.0, which splits in other forms with a clear antigenic community. A hydrolase was also observed with an IEP of 6.2. In this sense, secretin administration may also be said to induce a significant improvement in established acute pancreatitis, since it tended to normalize the structure and proportion of the proteins secreted.
authors: Departamento de Fisiología y Farmacología, Facultad de Biología, Universidad de Salamanca, Spain.
authors: M A
authors: MA
authors: Manso
authors: I
authors: I
authors: De Dios
authors: J I
authors: JI
authors: San Roman
authors: J J
authors: JJ
authors: Calvo
authors: M A
authors: MA
authors: Lopez
mesh_terms: D000208:Acute Disease; D000681:Amylases / Q000378:metabolism*; D000818:Animals; D002108:Ceruletide; D007525:Isoelectric Focusing; D008297:Male; D008970:Molecular Weight; D010189:Pancreatic Juice / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D010195:Pancreatitis / Q000139:chemically induced / Q000503:physiopathology*; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D012633:Secretin / Q000494:pharmacology*
chemical_list: D011506:Proteins; D012633:Secretin; D002108:Ceruletide; D000681:Amylases