title: A patch-clamp study of ion channels of inner and outer membranes and of contact zones of E. coli, fused into giant liposomes. Pressure-activated channels are localized in the inner membrane.
abstract: Inner and outer membranes of Escherichia coli and contact zones were isolated and fused separately with giant liposomes amenable to patch-clamp recording. Different types of large pressure-activated channels were localized in the inner membrane fraction which also contained smaller, pressure-insensitive channels. The outer membrane contained pressure-insensitive channels with large conductances and long opening and closing times which are likely to be porins. Large channels were also observed in contact zones.
authors: Laboratoire des Biomembranes, URA CNRS 1116, Orsay, France.
authors: C
authors: C
authors: Berrier
authors: A
authors: A
authors: Coulombe
authors: C
authors: C
authors: Houssin
authors: A
authors: A
authors: Ghazi
mesh_terms: D002458:Cell Fractionation; D002462:Cell Membrane / Q000502:physiology; D004553:Electric Conductivity; D004926:Escherichia coli / Q000502:physiology* / Q000648:ultrastructure; D006874:Hydrostatic Pressure; D007473:Ion Channels / Q000502:physiology*; D008081:Liposomes; D008564:Membrane Potentials
chemical_list: D007473:Ion Channels; D008081:Liposomes