Protein kinase C may regulate resting anion conductance in vascular smooth muscle cells.
The blocker of protein kinase C(PKC) activated large-conductance channel(337.1 pS) in cell attached patch mode in cultured vascular smooth muscle cells. The channel showed time-dependent inactivation whose time course became faster as the amplitude of the command potential was increased. These characteristics of large-conductance channel activated by the application of the PKC blocker were very similar to those of voltage-dependent Cl channels in these cells, indicating the channel activated by the drug is Cl channel. Since voltage-dependent Cl channels were reported to be only activated by forming inside-out patch, these findings suggest Cl permeability of vascular smooth muscle cells is at least partially regulated by protein kinase C.
Saigusa
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Department of Physiology, Jikei Univ. Sch. of Med., Tokyo, Japan.
Kokubun
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S
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000818:Animals; D002460:Cell Line; D002712:Chlorides / Q000378:metabolism; D004553:Electric Conductivity; D007473:Ion Channels / Q000378:metabolism; D007546:Isoquinolines / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology*; D010879:Piperazines / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism*; D013997:Time Factors
D002712:Chlorides; D007473:Ion Channels; D007546:Isoquinolines; D010879:Piperazines; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D011493:Protein Kinase C