1F7, a novel cell surface molecule, involved in helper function of CD4 cells.
We have developed a monoclonal antibody, anti-1F7, that inhibits soluble Ag-driven T cell proliferation as well as PWM-driven IgG synthesis. Anti-1F7 antibody reacts with approximately 57% of unfractionated T cells, 62% of CD4+ cells, and 54% of CD8+ cells. Although the 1F7 Ag is widely distributed among lymphoid cells, this Ag on CD4+ cells is preferentially expressed on the CDw29(4B4+) helper population. Moreover, anti-1F7 antibody further subdivides the CD4+CDw29+ cell subset into CDw29+1F7+ and CDw29+1F7- populations. The CD4+CDw29+1F7+ population of cells maximally proliferates to recall Ag such as tetanus toxoid, whereas helper function for PWM-driven IgG synthesis by B cells belongs to both the CD4+CDw29+1F7+ and CD4+CDw29+1F7- population of cells. The most prominent structure defined by this antibody is a 110-kDa molecule that is different from the 135-kDa, 160-kDa, and 185-kDa glycoproteins identified by anti-CDw29 antibody and the 180-kDa glycoprotein identified by UCHL-1 antibody. It is, however, related to the molecule recognized by anti-Ta1, an activation Ag on T cells. Furthermore, although the Ta1 molecule is recognized by anti-1F7 mAb, the 1F7 family of structures also includes molecules distinct from Ta1.
Morimoto
C
C
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, MA 02115.
Torimoto
Y
Y
Levinson
G
G
Rudd
C E
CE
Schrieber
M
M
Dang
N H
NH
Letvin
N L
NL
Schlossman
S F
SF
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000502:physiology; D000937:Antigen-Antibody Reactions; D000943:Antigens, Differentiation; D000954:Antigens, Surface / Q000276:immunology* / Q000302:isolation & purification; D001402:B-Lymphocytes / Q000378:metabolism; D001667:Binding, Competitive; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002469:Cell Separation; D002478:Cells, Cultured; D002675:Child, Preschool; D003429:Cross Reactions; D006649:Histocompatibility Antigens; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007223:Infant; D019012:Integrin beta1; D017493:Leukocyte Common Antigens; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype; D011043:Pokeweed Mitogens; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D000954:Antigens, Surface; D006649:Histocompatibility Antigens; D007074:Immunoglobulin G; D019012:Integrin beta1; D011043:Pokeweed Mitogens; D017493:Leukocyte Common Antigens