title: Complete staining of human spermatozoa and immature germ cells combined with phase contrast microscopy.
abstract: A method combining Janus green B and Thymol blue stains the anterior part of the head, the nuclear membrane, middle piece, and tail of spermatozoa light green and the nucleus deep purple. The method provides excellent stained preparations for the evaluation of sperm morphology by phase contrast microscopy. It produces significantly less abnormal spermatozoa compared with the Papanicolaou stain.
authors: Department of Gynecology and Obstetrics, Hvidovre Hospital, University of Copenhagen, Denmark.
authors: A Y
authors: AY
authors: Michael
authors: J O
authors: JO
authors: Drejer
authors: P V
authors: PV
authors: Bagger
authors: G U
authors: GU
authors: Detlefsen
authors: G
authors: G
authors: Stakemann
mesh_terms: D001391:Azo Compounds; D006801:Humans; D008297:Male; D008858:Microscopy, Phase-Contrast; D010635:Phenolphthaleins; D013094:Spermatozoa / Q000166:cytology*; D013194:Staining and Labeling; D013944:Thymolphthalein / Q000031:analogs & derivatives
chemical_list: D001391:Azo Compounds; D010635:Phenolphthaleins; C013713:thymol blue; C005086:Janus Green B; D013944:Thymolphthalein