Low-cytopathic infectious clone of human immunodeficiency virus type I (HIV-I).
Single genotypic variants of HIV-I, contained in a parental cytopathic HIV-I isolate, were isolated by molecular cloning and propagated in susceptible cells. Two such HIV-I clones, designated N1T-E and N1T-A, exhibited similar restriction endonuclease maps but strikingly different biological activities. Infection of T lymphocytes or monocytes by clone N1T-E was characterized by slow kinetics and lack of significant cytopathic effects, but high reverse transcriptase activity levels in culture supernatants of chronically-infected cells. Clone N1T-A, like the parental HIV-I isolate, exhibited fast kinetics of infection in T cells and monocytes and strong cytopathicity in these cells. Full characterization of the low-cytopathic virus in comparison to the structurally similar cytopathic clone may facilitate the elucidation of the molecular basis of HIV cytopathogenicity.
Sakai
K
K
Molecular Virology Laboratory, St. Luke's/Roosevelt Hospital Center, College of Physicians & Surgeons, Columbia University, New York, NY 10019.
Ma
X Y
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Volsky
D J
DJ
D003001:Cloning, Molecular; D003588:Cytopathogenic Effect, Viral; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D014644:Genetic Variation; D005838:Genotype; D006678:HIV / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D009000:Monocytes / Q000382:microbiology; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013601:T-Lymphocytes / Q000382:microbiology
D004274:DNA, Recombinant; D012194:RNA-Directed DNA Polymerase; D004262:DNA Restriction Enzymes