Changes in ribonucleoprotein particle and chromatin organization induced by liposomes in isolated nuclei.
Nuclei isolated from rat liver, incubated in the presence of liposomes of different phospholipids, undergo typical modifications: chromatin dispersion and reduction of the interchromatin granules in nuclei incubated with negatively charged liposomes and increase of the chromatin density and of the number and size of the interchromatin granules in nuclei incubated with neutral liposomes. The possibility that the observed modifications are caused by an impairment of the transport and translocation of ribonucleoproteins belonging to the inner nuclear matrix, is suggested by the results obtained by radiotracer techniques on the release of RNA from liposome-incubated nuclei.
Maraldi
N M
NM
Istituto di Citomorfologia Normale e Patologica CNR, Istituto Rizzoli, Bologna, Italy.
Galanzi
A
A
Caramelli
E
E
Billi
A M
AM
Ognibene
A
A
Rizzoli
R
R
Capitani
S
S
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects* / Q000648:ultrastructure; D002843:Chromatin / Q000648:ultrastructure*; D008081:Liposomes / Q000494:pharmacology*; D008854:Microscopy, Electron; D010713:Phosphatidylcholines / Q000494:pharmacology; D010718:Phosphatidylserines / Q000494:pharmacology; D012313:RNA / Q000378:metabolism; D051381:Rats; D012261:Ribonucleoproteins / Q000502:physiology*
D002843:Chromatin; D008081:Liposomes; D010713:Phosphatidylcholines; D010718:Phosphatidylserines; D012261:Ribonucleoproteins; D012313:RNA