Concentration of Babesia bigemina-infected erythrocytes using a dextran sulphate affinity column.
Babesia bigemina-infected erythrocytes preferentially bind to dextran sulphate affinity columns. Subsequent elution yields suspensions containing up to 95% infected erythrocytes. Preliminary immunoblotting studies indicate a parasite antigen of 35,000 mol. wt might be implicated in the binding.
Goodger
B V
BV
Bushell
G
G
Wright
I G
IG
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis; D001403:Babesia / Q000276:immunology*; D001404:Babesiosis / Q000097:blood*; D002417:Cattle; D002846:Chromatography, Affinity; D016264:Dextran Sulfate; D003911:Dextrans / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism / Q000469:parasitology*; D015151:Immunoblotting
D000953:Antigens, Protozoan; D003911:Dextrans; D016264:Dextran Sulfate