[Inhibitory effect of various 3'-amino- and 3'-azido-3'-deoxyribonucleotide-5'-triphosphates on RNA synthesis, catalyzed by RNA polymerase of influenza type A virus and cellular RNA-polymerase].
Some new analogues of ribonucleoside-5'-triphosphates modified in 3'-ribose position and base [CTP (3'NH2), CTP (3'NH2) (5Me), CTP (3'N3) (5 Me), RvTP (3'N3)] have been synthesized. The inhibitions of RNA-synthesis catalyzed by the influenza A viral RNA-polymerase in cell free system and by the RNA-polymerase II from mice liver in the system of cellular nuclei by these reagents have been compared. All the studied preparations efficiently inhibited the RNA-synthesis in both cases. The inhibitors modified only in 3'-ribose position did not express specificity to any of RNA-polymerases tested, while some analogues having two modification in the molecule demonstrated the selective inhibition of RNA-synthesis directed by the influenza A viral RNA-polymerase [ara GTP (3'NH2), RvTP (3'N3')].
Pravdina
N F
NF
Papchikhin
A V
AV
Purygin
P P
PP
Galegov
G A
GA
D000818:Animals; D002384:Catalysis; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D003854:Deoxyribonucleotides / Q000494:pharmacology*; D009980:Influenza A virus / Q000201:enzymology*; D008099:Liver / Q000201:enzymology; D051379:Mice; D012313:RNA / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000187:drug effects
D003854:Deoxyribonucleotides; D012313:RNA; D012321:DNA-Directed RNA Polymerases
Ingibiruiushchee deĭstvie nekotorykh 3'-amino- i 3'-azido-3'-dezoksiribinukleozid-5'-trifosfatov na sintez RNK, kataliziruemyĭ RNK-polimeraznoĭ virusa grippa A i kletochnoĭ RNK-polimerazoĭ.