Stimulation of gut-associated lymphoid cells by IL-4 and B-cell growth factor II.
We have analysed the ability of B cells isolated from the Peyer's patches of normal mice to respond to two different B-cell stimulatory factors. Peyer's patch B cells respond in vitro to co-stimulation by either interleukin-4 (IL-4) plus anti-IgM or B-cell growth factor II (BCGF-II) plus dextran sulphate (DXS). We have consistently observed that splenic B cells proliferate more than Peyer's patch B cells when co-stimulated by BCGF-II plus DXS. This is not due to a diminished proliferation capacity of the Peyer's patch B-cell preparations, because the Peyer's patch B cells often proliferate more than splenic B cells when co-stimulated with IL-4 plus anti-IgM. These differences are not a result of altered response kinetics or differences in relative amounts of surface IgM on the two types of B cells. We have also analysed B cells from the LPS hypo-responsive strain C3H/HeJ, and its LPS responsive partner strain C3H/OuJ, from the X-linked immunodeficient strain (xid), CBA/N, and from germ-free (GF) mice. B cells from spleens and Peyer's patches of GF mice are responsive to co-stimulation by IL-4 plus anti-IgM and to co-stimulation by BCGF-II plus DXS. Peyer's patch B cells from C3H/HeJ mice proliferate as well as Peyer's patch B cells from C3H/OuJ cells to both co-stimulation protocols. Among the types of B cells studied here, only cells from spleens and Peyer's patches of mice that bear the xid defect fail to respond to the signals delivered by lymphokine co-stimulation. Our results suggest that while Peyer's patch B cells are stimulated by these two lymphokines, Peyer's patches contain cells that react differently from spleen cells to either the lymphokines IL-4 and BCGF-II, or the co-stimulators anti-IgM or dextran sulphate.
Tonkonogy
S L
SL
Department of Microbiology, Pathology and Parasitology, School of Veterinary Medicine, North Carolina State University, Raleigh 27606.
Swain
S L
SL
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D016264:Dextran Sulfate; D003911:Dextrans / Q000494:pharmacology; D007075:Immunoglobulin M / Q000276:immunology; D015848:Interleukin-5; D007378:Interleukins / Q000276:immunology* / Q000494:pharmacology*; D008213:Lymphocyte Activation; D051379:Mice; D008815:Mice, Inbred Strains; D008938:Mitosis; D010581:Peyer's Patches / Q000276:immunology; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D013154:Spleen / Q000276:immunology; D013997:Time Factors
D000888:Antibodies, Anti-Idiotypic; D003911:Dextrans; D007075:Immunoglobulin M; D015848:Interleukin-5; D007378:Interleukins; D011947:Receptors, Antigen, B-Cell; D016264:Dextran Sulfate