[Activity of lipoxygenase incorporated into reversed micelles of aerosol OT in octane].
Soybean lipoxygenase (EC 1.13.11.12) incorporated into the reversed micelles of aerosol OT in octane has been studied for its catalytic properties. The enzyme is shown to preserve up to 10% activity as compared with the activity in the aqueous solution. In this case Km of lipoxygenase for linoleic acid increases from 10(-5) M to 5 X 10(-4) M. The activity of lipoxygenase is maximal, the aerosol OT concentration being 0.03 M and a degree of reversed micelle hydratation 40. Cationic detergents of the cetyltrimethyl ammonium bromide type are not good to form reversed micelles of lipoxygenase, since they inhibit the latter with IC50 = (4 divided by 6) x 10(-4) M. The lipoxygenase preparations in reversed micelles of aerosol OT in octane may be used to synthesize natural metabolites of polyunsaturated fatty acids, for instance of eicosanoids.
Butovich
I A
IA
Mogilevich
T V
TV
Kukhar'
V P
VP
D003102:Colloids; D004143:Dioctyl Sulfosuccinic Acid; D006863:Hydrogen-Ion Concentration; D008084:Lipoxygenase / Q000378:metabolism*; D008823:Micelles; D010084:Oxidation-Reduction; D013025:Glycine max / Q000201:enzymology*; D013056:Spectrophotometry, Ultraviolet; D013386:Succinates
D003102:Colloids; D008823:Micelles; D013386:Succinates; D004143:Dioctyl Sulfosuccinic Acid; D008084:Lipoxygenase
Aktivnost' lipoksigenazy, vkliuchennoĭ v obrashchennye mitselly aérozolia OT v oktane.