Activation-driven T cell death. I. Requirements for de novo transcription and translation and association with genome fragmentation.
We have observed that stimuli that are mitogenic for normal T cells can induce cell death in transformed T cell hybridomas. "Activation-driven cell death" can be triggered by the presentation of appropriate Ag as well as by treatment with lectins and antibodies specific for the T cell Ag receptor complex and other activation structures on the T cell surface, such as Thy-1 and Ly-6. The activation-driven lethal process is cell autonomous, is associated with a fragmentation of the cell's genome characteristic of the "suicide process" induced in immature T cells by glucocorticoids and in target cells by cytotoxic T lymphocytes, and is dependent upon transcription and translation, presumably associated with the expression of new gene products. We hypothesize that activation-driven cell death may be involved in vivo in the clonal deletion of auto-reactive T cells during T cell ontogeny.
Ucker
D S
DS
Division of Immunology, Medical Biology Institute, La Jolla, CA 92037.
Ashwell
J D
JD
Nickas
G
G
D000818:Animals; D000918:Antibody Specificity; D000950:Antigens, Ly / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D002470:Cell Survival; D003602:Cytotoxicity, Immunologic; D004249:DNA Damage; D000939:Epitopes / Q000276:immunology; D006825:Hybridomas / Q000276:immunology / Q000378:metabolism; D007700:Kinetics; D037102:Lectins / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D014176:Protein Biosynthesis; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000378:metabolism; D018800:Thy-1 Antigens; D014158:Transcription, Genetic
D000950:Antigens, Ly; D000954:Antigens, Surface; D000939:Epitopes; D037102:Lectins; D011948:Receptors, Antigen, T-Cell; D018800:Thy-1 Antigens