Scirrhous carcinoma cell invasion into the stomach wall detected by monoclonal antibody S202: a comparison between immunoperoxidase and hematoxylin-eosin stain.
We used anti-scirrhous carcinoma monoclonal antibody (MAb) S202, which reacted strongly with scirrhous gastric cancer, to evaluate the depth of cancer cell invasion into the stomach wall. Sixty-eight scirrhous gastric cancer specimens were stained with the avidin-biotin peroxidase complex method using MAb S202. There were some discrepancies between the histological evaluation of the immunoperoxidase (IP) and hematoxylin-eosin (HE) staining regarding the depth of tumor invasion. In two of the 14 HE staining cases in which carcinoma had extended as far as the proper muscular layer, IP staining revealed that the lesions had actually penetrated this layer. As well, in two of the 18 HE staining cases in which carcinoma cells had not penetrated as far as the serosa, IP staining revealed that they had, in fact, reached the serosal surface. The invasive carcinoma cells were more easily discerned using IP staining with MAb S202 rather than using histological staining with HE. Thus, MAb S202 may be useful for precise identification of carcinoma cells.
Yokota
T
T
First Department of Surgery, Kyoto Prefectural University of Medicine, Japan.
Takahashi
T
T
Yamaguchi
T
T
Sawai
K
K
Hagiwara
A
A
Shimotsuma
M
M
Doi
M
M
Masuko
T
T
Hashimoto
Y
Y
D002293:Adenocarcinoma, Scirrhous / Q000473:pathology*; D000911:Antibodies, Monoclonal; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin; D006801:Humans; D007124:Immunoenzyme Techniques; D009361:Neoplasm Invasiveness; D013194:Staining and Labeling; D013274:Stomach Neoplasms / Q000473:pathology*
D000911:Antibodies, Monoclonal; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin