Regulation of components of the Pseudomonas aeruginosa phosphate-starvation-inducible regulon in Escherichia coli.
Plasmids pPBP and pRS-XP containing the cloned genes for the Pseudomonas aeruginosa phosphate-starvation-inducible periplasmic phosphate-binding protein and outer membrane porin P (oprP), respectively, were introduced into various Escherichia coli Pho-regulon regulatory mutants. Using Western immunoblots and specific antisera, the production of both gene products was observed to be under the control of regulatory elements of the E. coli Pho regulon. Sequencing of the region upstream of the translational start site of the oprP gene revealed a 'Pho box' with strong homology to the E. coli consensus 'Pho box', the putative binding site of the PhoB activator. Since P. aeruginosa and E. coli belong to different families and have quite different GC contents, these data suggest strong evolutionary conservation of regulatory elements of the Pho regulon.
Siehnel
R J
RJ
Department of Microbiology, University of British Columbia, Vancouver, Canada.
Worobec
E A
EA
Hancock
R E
RE
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D007158:Immunologic Techniques; D007473:Ion Channels / Q000378:metabolism; D008969:Molecular Sequence Data; D028044:Phosphate-Binding Proteins; D010710:Phosphates / Q000378:metabolism* / Q000502:physiology; D010957:Plasmids; D018272:Porins; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D014169:Transformation, Bacterial
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; D007473:Ion Channels; D028044:Phosphate-Binding Proteins; D010710:Phosphates; D018272:Porins; D014157:Transcription Factors