The resistance to alkali treatment of the phosphorylation of beta casein versus alpha casein is specific for casein kinase II.
The phosphorylation of mixed casein by casein kinase II shows resistance on beta casein after partial alkali hydrolysis of the proteins separated by gel electrophoresis. This property is specific for casein kinase II among the protein kinases tested and can be used for casein kinase II detection in biological extracts and for characterization of purified casein kinase II.
Mulner-Lorillon
O
O
Physiologie de la Reproduction, CNRS UA 555, Paris, France.
Creuzet
C
C
Bellé
R
R
Loeb
J
J
D000468:Alkalies; D000818:Animals; D047388:Casein Kinases; D002364:Caseins / Q000378:metabolism*; D066298:In Vitro Techniques; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*
D000468:Alkalies; D002364:Caseins; D011494:Protein Kinases; D047388:Casein Kinases