title: Kinetic analysis of glutamate-induced chloride current in Aplysia neurones: a 'concentration clamp' study.
abstract: L-Glutamate (Glu) applied by the 'concentration clamp' technique to isolated neurones of Aplysia induced a chloride current (ICl) by activating a single population of the channel. The concentration-response curve for the peak ICl gave a dissociation constant of 1.3 x 10(-4) M and a Hill coefficient of 1.8. The current-voltage relationship was linear in the voltage range examined (-60 to +10 mV). The activation phase of the ICl followed a single-exponential time course and desensitization was complete with a double-exponential time course. The time constants for activation and desensitization decreased with increasing concentrations of Glu but were voltage-independent. The process of recovery from desensitization was also double-exponential. The single-channel conductance estimated by ensemble noise analysis was 50 +/- 4.7 pS (n = 4). These results suggest that the Glu receptor-Cl channel complex in Aplysia neurones consists of a single population with two binding sites for the agonist.
authors: Department of Physiology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
authors: Y
authors: Y
authors: Ikemoto
authors: N
authors: N
authors: Akaike
authors: K
authors: K
authors: Ono
mesh_terms: D000818:Animals; D001048:Aplysia / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism*; D005971:Glutamates / Q000494:pharmacology*; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects; D007700:Kinetics; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*
chemical_list: D002712:Chlorides; D005971:Glutamates; D007473:Ion Channels