Molecular understanding of cellular adhesion on artificial surfaces.
This study was conducted to clarify cellular adhesion mechanisms of blood cells (platelets [PLT] and white blood cells [WBC]) and vascular endothelial cells at the molecular level. This study indicated that the adhesion of three cellular systems to proteins such as fibronectin and fibrinogen proceeds via the RGD (Arg-Gly-Asp) ligand-receptor interaction, in which the RGD tripeptidyl sequence is the minimal amino acid sequence common to adhesive proteins. This was evident from the dose-dependent inhibitory effect of RGD-containing peptide on cellular adhesion. Additional supporting evidence was the presence of PLT and WBC receptors, which molecularly recognize RGD, verified by fluorescein-labelled RGD-containing peptide. The adhesion of vascular endothelial cells was also predominantly controlled by the ligand-receptor mechanism, and participation of complement activation on WBC adhesion was demonstrated as well. The adhesion of WBCs on surface hydroxyl group-bearing polymers proceeded via the CR3 receptor-C3b ligand interaction, in which activated complement factor C3b is chemically fixed upon complement activation. Thus, the molecular understanding of cellular adhesion mechanisms provide the basis of biocompatibility for implantation and extracorporeal circulation, as well as molecular design of artificial and bioartificial organs.
Takemoto
Y
Y
Department of Bioengineering, National Cardiovascular Center Research Institute, Osaka, Japan.
Matsuda
T
T
Kishimoto
T
T
Maekawa
M
M
Akutsu
T
T
D000818:Animals; D001792:Blood Platelets / Q000502:physiology*; D002417:Cattle; D002448:Cell Adhesion / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000502:physiology*; D003094:Collagen / Q000502:physiology; D003167:Complement Activation / Q000502:physiology; D004730:Endothelium, Vascular / Q000502:physiology*; D005340:Fibrinogen / Q000502:physiology; D005353:Fibronectins / Q000502:physiology; D006023:Glycoproteins / Q000502:physiology; D006801:Humans; D007797:Laminin / Q000502:physiology; D007962:Leukocytes / Q000502:physiology*; D009842:Oligopeptides / Q000502:physiology; D011971:Receptors, Immunologic / Q000502:physiology; D018000:Receptors, Peptide; D019096:Vitronectin; D014841:von Willebrand Factor / Q000502:physiology
D015815:Cell Adhesion Molecules; D005353:Fibronectins; D006023:Glycoproteins; D007797:Laminin; D009842:Oligopeptides; D011971:Receptors, Immunologic; D018000:Receptors, Peptide; D019096:Vitronectin; C051382:arginyl-glycyl-aspartic acid directed cell adhesion receptor; D014841:von Willebrand Factor; C047981:arginyl-glycyl-aspartic acid; D005340:Fibrinogen; D003094:Collagen; C046617:arginyl-glycyl-aspartyl-serine