Allo-class I-reactive CD4-CD8- T cell hybridomas recognize the conformational change of class I molecule resulting from the exchange of the alpha 3 domain.
H-2Kb-reactive, interleukin (IL) 2-producing T cell hybridomas possessing neither CD8 nor CD4 molecules were employed for the study of allorecognition on interspecies hybrid antigens by T cells in the absence of an influence of these accessory molecules. Both HTB176.10 and HTB177.2 T cell hybridomas reacted with KbKbB7 hybrid antigens as well as Kb antigens and they produced more IL2 in response to Kb antigens than KbKbB7 hybrid antigens. IL2 production of these T cell hybridomas was dependent on the surface expression level of Kb molecules on stimulators. Therefore, L cells expressing almost equivalent levels of Kb or hybrid antigens were selected for further functional studies by these T cell hybridomas. They apparently produced less IL2 in response not only to interspecies hybrid antigens but also to interspecies hybrid antigens in response to Kb antigens. These results indicated that T cell hybridomas recognized the conformational change of class I molecule resulting from the exchange of the alpha 3 domain via their T cell receptors.
Yamamoto
J
J
Department of Immunology, School of Medicine, University of Tokyo, Japan.
Tanabe
M
M
Minami
M
M
Kano
K
K
Takiguchi
M
M
D000595:Amino Acid Sequence; D000818:Animals; D000945:Antigens, Differentiation, T-Lymphocyte / Q000502:physiology; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens; D000939:Epitopes; D006183:H-2 Antigens / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000032:analysis / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D007376:Interleukin-2 / Q000096:biosynthesis; D051379:Mice; D011487:Protein Conformation; D013601:T-Lymphocytes / Q000276:immunology*
D000945:Antigens, Differentiation, T-Lymphocyte; D015704:CD4 Antigens; D016827:CD8 Antigens; D000939:Epitopes; D006183:H-2 Antigens; D015395:Histocompatibility Antigens Class I; D007376:Interleukin-2