Delaying virus maturation for a higher yield of cell associated virus antigens.
Negligible amounts of virus were released when infected cells were maintained in low ionic strength medium. The cells accumulated a high titre of virus and its specific antigenic proteins, detected by complement-fixation test. The procedure has potential for production of virus subunit vaccines.
Qureshi
A A
AA
Department of Biology, University of Bahrain, Arabian Gulf.
D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis*; D002460:Cell Line; D003168:Complement Fixation Tests; D003470:Culture Media; D003716:Dengue Virus / Q000254:growth & development / Q000276:immunology; D004664:Encephalitis Virus, Japanese / Q000254:growth & development / Q000276:immunology; D004665:Encephalitis Virus, St. Louis / Q000254:growth & development / Q000276:immunology; D000939:Epitopes / Q000032:analysis; D005416:Flavivirus / Q000254:growth & development / Q000276:immunology*; D014902:West Nile virus / Q000254:growth & development / Q000276:immunology
D000956:Antigens, Viral; D003470:Culture Media; D000939:Epitopes