Human isolated small intestine: motor responses of the longitudinal muscle to field stimulation and exogenous neuropeptides.
(1) Longitudinal muscle strips from the human small intestine (jejunum/ileum) responded to electrical field stimulation (1-50 Hz) with frequency-related primary contractions which were largely atropine- (3 microM) sensitive. When the tone was raised by addition of galanin (0.3-1 microM), prostaglandin (PG) E2 (1-10 microM) or neurokinin A (NKA, 0.1 microM), a frequency-related relaxation was evident which was potentiated by atropine. All the responses to field stimulation were abolished by tetrodotoxin (1 microM), thus indicating their neural origin. (2) The atropine-sensitive primary contraction to field stimulation was virtually abolished by omega conotoxin fraction GVIA (CTX, 0.1-0.3 microM) while the relaxations were CTX-resistant. The field stimulation-induced relaxations, which were observed in the presence of atropine and guanethidine (3 microM), were also unaffected by apamin (0.1 microM). (3) NKA and substance P (SP) produced a concentration- (1 nM-1 microM for both peptides) related contraction, NKA being about 53 times more potent than SP. [Pro9]SP sulphone and [MePhe7]-NKB, selective agonists of the NK-1 and NK-3 receptor, respectively, were barely effective. On the other hand, [beta Ala8]NKA(4-10), a selective NK-2 receptor agonist, had a potent contractile activity, similar to that of NKA. (4) Galanin (1 nM-1 microM) produced an atropine- and tetrodotoxin-resistant concentration-related contraction of longitudinal muscle of human isolated small intestine. The response to galanin did not show any sign of fading and was particularly suitable to study the evoked relaxations.(ABSTRACT TRUNCATED AT 250 WORDS)
Maggi
C A
CA
Pharmacology Department, A. Menarini Pharmaceuticals, Florence, Italy.
Patacchini
R
R
Santicioli
P
P
Giuliani
S
S
Turini
D
D
Barbanti
G
G
Beneforti
P
P
Misuri
D
D
Meli
A
A
D000368:Aged; D001285:Atropine / Q000494:pharmacology; D015740:Calcitonin Gene-Related Peptide; D004558:Electric Stimulation; D005260:Female; D019004:Galanin; D006801:Humans; D066298:In Vitro Techniques; D007421:Intestine, Small / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D009119:Muscle Contraction / Q000187:drug effects; D009126:Muscle Relaxation / Q000187:drug effects; D009130:Muscle, Smooth / Q000502:physiology*; D009479:Neuropeptides / Q000494:pharmacology*; D010197:Pancuronium / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology; D015320:Tachykinins / Q000494:pharmacology; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
D009479:Neuropeptides; D010455:Peptides; D015320:Tachykinins; D014660:Vasoactive Intestinal Peptide; D001285:Atropine; D019004:Galanin; D010197:Pancuronium; D015740:Calcitonin Gene-Related Peptide