title: Antigenicity of the peplomer protein of infectious bronchitis virus.
abstract: To study the antigenic structure of the peplomer protein of the avian coronavirus infectious bronchitis virus, fragments from the peplomer gene were generated by restriction-enzyme cleavage or by limited DNase digestion and inserted in the Escherichia coli expression plasmid pEX (Stanley and Luzio, 1984). The antigenicity of the expression products was tested using a number of polyclonal antisera and monoclonal antibodies. The polyclonal antisera recognized different sets of epitopes in the 1162-residue sequence. The N-terminal region of one of the two subunits, S2, was recognized by all polyclonal sera and by two monoclonal antibodies. This clearly immunodominant region contains at least two adjacent or overlapping epitopes, one of which has been localized within 18 residues. The epitopes found as antigenic pEX expression products do not coincide with the regions in the S1 subunit that have been found to contain hypervariable sequences. We suggest that these regions constitute conformation dependent neutralization epitopes that cannot be detected in the pEX system. The relevance of our findings for vaccine development is discussed.
authors: Institute of Infectious Diseases and Immunology, Veterinary Faculty, State University, Utrecht, The Netherlands.
authors: J A
authors: JA
authors: Lenstra
authors: J G
authors: JG
authors: Kusters
authors: G
authors: G
authors: Koch
authors: B A
authors: BA
authors: van der Zeijst
mesh_terms: D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000032:analysis*; D001483:Base Sequence; D015153:Blotting, Western; D003332:Coronaviridae / Q000276:immunology*; D000939:Epitopes / Q000032:analysis; D005814:Genes, Viral; D001351:Infectious bronchitis virus / Q000235:genetics / Q000276:immunology*; D015183:Restriction Mapping; D014759:Viral Envelope Proteins / Q000276:immunology*
chemical_list: D000956:Antigens, Viral; D000939:Epitopes; D014759:Viral Envelope Proteins