NMR studies in the relapsing experimental allergic encephalomyelitis (EAE) model of multiple sclerosis in the strain 13 guinea pig.
Juvenile strain 13 guinea pigs sensitized with an emulsion of whole isologous central nervous system (CNS) tissue in complete Freund's adjuvant in the first two weeks of life develop a relapsing-remitting form of experimental allergic encephalomyelitis (EAE) which resembles multiple sclerosis (MS) both clinically and pathologically. In order to determine if this experimental model could be used to identify the tissue factors which contribute to the magnetic resonance imaging (MRI)-detected lesions in MS, we measured T1 and T2 relaxation times, tissue specific gravity and histology throughout the entire CNS in vitro in eighteen animals during the acute phase (first attack), and twenty-one animals during further periods of clinical worsening (relapses) and recovery (remissions). The neuropathological findings of spinal cord meningeal inflammation, perivascular and parenchymal infiltration (myelitis and encephalitis) and demyelination were more marked during periods of clinical worsening than when the animal had recovered clinically. Even though the histological changes of EAE were present in all experimental animals, NMR relaxation times and tissue specific gravity could not distinguish experimental (first attack, subsequent relapses and remissions) from control animals due to a wide range of values for each of these parameters. Although relapsing EAE in the strain 13 guinea pig has been an instructive model of MS, we have found that in vitro NMR relaxometry cannot be used to predict the presence or degree of pathological change in the nervous system of these experimental animals.
Karlik
S J
SJ
Department of Diagnostic Radiology and Nuclear Medicine, University of Western Ontario, London, Canada.
Wong
C
C
Gilbert
J J
JJ
Noseworthy
J H
JH
D000818:Animals; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000175:diagnosis*; D006168:Guinea Pigs; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D009103:Multiple Sclerosis / Q000175:diagnosis*; D004676:Myelin Basic Protein / Q000032:analysis
D004676:Myelin Basic Protein