title: beta-Xyloside effects on basal lamina structure and anionic site distribution the embryonic mouse submandibular salivary gland.
abstract: beta-D-Xyloside is a proteoglycan biosynthesis inhibitor. Previous studies on embryonic salivary glands have demonstrated that 0.5 mM beta-xyloside (1) inhibits proteoglycan synthesis by 50%; (2) severely depresses sulphated glycosaminoglycan deposition at the basal epithelial surface, and (3) dramatically inhibits epithelial branching morphogenesis. Electron microscopy revealed a conventional three-layered basal lamina that is altered in the presence of beta-xyloside by a 35% reduction in the number of tannic acid-resolved particles in the lamina densa. Basal lamina anionic sites, resolved with ruthenium red (RR) and polyethyleneimine (PEI) cationic probes, were also reduced in the presence of beta-xyloside. PEI particles were reduced by 28%, and RR particles by 24%, per two-dimensional unit of basal lamina. These beta-xyloside effects on anionic sites are consistent with an hypothesis that sulphated glycosaminoglycans account for 50% of the basal lamina anionic sites and a predicted 25% decrease in anionic sites in the presence of beta-xyloside.
authors: Division of Biology, Kansas State University, Manhattan 66506.
authors: B S
authors: BS
authors: Spooner
authors: A
authors: A
authors: Paulsen
authors: M S
authors: MS
authors: Sullins
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000187:drug effects / Q000196:embryology* / Q000648:ultrastructure; D002478:Cells, Cultured; D005260:Female; D006025:Glycosaminoglycans / Q000096:biosynthesis; D006027:Glycosides / Q000494:pharmacology*; D047348:Hydrolyzable Tannins; D007473:Ion Channels / Q000187:drug effects*; D051379:Mice; D008854:Microscopy, Electron; D011094:Polyethyleneimine; D012430:Ruthenium Red; D013363:Submandibular Gland / Q000187:drug effects / Q000196:embryology* / Q000648:ultrastructure
chemical_list: D006025:Glycosaminoglycans; D006027:Glycosides; D047348:Hydrolyzable Tannins; D007473:Ion Channels; C010807:xylosides; D012430:Ruthenium Red; D011094:Polyethyleneimine