title: A monoclonal antibody (MSN-1) against a newly established uterine endometrial cancer cell line (SNG-II) and its application to immunohistochemistry and flow cytometry.
abstract: To determine a phenotypic difference between normal endometrium and endometrial adenocarcinoma, a new monoclonal antibody (MSN-1) was produced by immunizing a new endometrial cancer cell line (SNG-II), which was established in 1981 from a 43-year-old Japanese woman with stage II uterine endometrial cancer. MSN-1 recognized the Lewis-b carbohydrate moiety on the cell surface glycolipid and seldom reacted immunohistochemically with normal endometrium but with about 90% of endometrial cancer cases. By application of MSN-1 to flow cytometry, the possibility of differentiating endometrial normal cells from cancer cells was demonstrated.
authors: Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, Japan.
authors: S
authors: S
authors: Nozawa
authors: M
authors: M
authors: Sakayori
authors: K
authors: K
authors: Ohta
authors: R
authors: R
authors: Iizuka
authors: H
authors: H
authors: Mochizuki
authors: M
authors: M
authors: Soma
authors: J
authors: J
authors: Fujimoto
authors: J
authors: J
authors: Hata
authors: M
authors: M
authors: Iwamori
authors: Y
authors: Y
authors: Nagai
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis* / Q000235:genetics; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis; D000939:Epitopes; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D007621:Karyotyping; D009367:Neoplasm Staging; D011003:Ploidies; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms / Q000175:diagnosis* / Q000235:genetics
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000939:Epitopes