Specific binding and release of cells from beads using cleavable tetrameric antibody complexes.
A two-step separation procedure is described for the positive selection of cells based on their reactivity with mouse monoclonal antibodies. In the first step cells are specifically cross-linked to hapten-modified glass beads using tetrameric monoclonal antibody complexes. In the second step bound cells are selectively eluted by reductive cleavage of the tetrameric antibody complexes. The latter are comprised of two mouse IgG1 monoclonal antibodies (one recognizing a cell surface antigen on target cells and the other a hapten coupled to the glass beads) bound together by two F(ab')2 fragments of rat anti-mouse IgG1 monoclonal antibody. The complexes provide a specific cleavable cross-link between cell and bead because the disulfide bonds between the two Fab' arms of the F(ab')2 fragments can be broken under relatively mild conditions using dithiothreitol. This specific cleavage of the cross-linker allows elution of the specifically absorbed cells without co-elution of non-specifically bound cells. This is shown in the purification of CD3+ T cells from human peripheral blood, where the removed fractions were over 90% pure and approximately 50% of the positive cells were recovered. Separation of cells labelled with limiting amounts of tetrameric antibody complexes demonstrated that this separation technique was also effective for the purification of cells expressing low amounts of antigens. This was confirmed by the purification of CD34-positive cells from human bone marrow. With this approach, colony-forming cells were enriched 15-24-fold over density separated marrow.
Thomas
T E
TE
Terry Fox Laboratory, Cancer Control Agency of B.C., Vancouver, Canada.
Sutherland
H J
HJ
Lansdorp
P M
PM
D000911:Antibodies, Monoclonal / Q000276:immunology*; D018952:Antigens, CD34; D000943:Antigens, Differentiation / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D017252:CD3 Complex; D016827:CD8 Antigens; D002469:Cell Separation / Q000379:methods*; D004229:Dithiothreitol / Q000494:pharmacology; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D007163:Immunosorbent Techniques; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000276:immunology; D046911:Macromolecular Substances; D011948:Receptors, Antigen, T-Cell / Q000276:immunology
D000911:Antibodies, Monoclonal; D018952:Antigens, CD34; D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D016827:CD8 Antigens; D007140:Immunoglobulin Fab Fragments; D046911:Macromolecular Substances; D011948:Receptors, Antigen, T-Cell; D004229:Dithiothreitol