[Preparation of a suspension of isolated hepatocytes using the Soviet drug collalitin].
Isolation of individual hepatocytes suspension from rats was carried out using trypsin, collagenase and collalytine; maximal yield of the viable hepatocytes was obtained when 0.05% solution of collagenase was used. Trypsin was ineffective in production of viable cells, while collalytine from pig pancreas exhibited the satisfactory results at concentration of 0.15-0.20%.
Toshchakov
V Iu
VIu
Danilov
M A
MA
Shestakova
N K
NK
Kozlovskaia
T I
TI
Kulikova
E Iu
EIu
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D008099:Liver / Q000166:cytology*; D003012:Microbial Collagenase; D051381:Rats; D013194:Staining and Labeling; D014357:Trypsin
D014357:Trypsin; D003012:Microbial Collagenase
Poluchenie suspenzii izolirovannykh gepatotsitov s pomoshch'iu otechestvennogo preparata kollalitina.