Monoclonal antibody to a supertypic determinant associated with HLA-DRw52.
A cytotoxic mouse monoclonal antibody UL-52 (IgG2b) was obtained after immunization of a BALB/c mouse with the lymphoblastoid cell line STA homozygous for HLA-A3, B8, Cw7, DR3, DRw52, DQw2, DPw2. Fluorescence analysis on a panel of B lymphoblastoid cell lines from the 10th International Histocompatibility Workshop 1987 showed almost exact concordance of UL-52 reactivity with the presence of the HLA-DRw52 antigen. Cytotoxicity testing of UL-52 on mononuclear cells of HLA-typed individuals revealed a pattern of reactivity closely associated with the HLA-DRw52 specificity as defined by conventional alloantisera (R = 0.77). UL-52 precipitated appropriate 29,000- and 33,000-dalton bands on SDS- polyacrylamide gels under reducing conditions from an HLA-DRw52 positive B lymphoblastoid cell line. Thus, by serological and biochemical criteria UL-52 defines a supertypic determinant associated with HLA-DRw52. In contrast to most DRw52- like monoclonal antibodies, UL-52 binds to DRw8 positive cells.
Eiermann
T H
TH
Department of Transplantation Immunology, Red Cross Blood Bank Ulm, West Germany.
Krause
S
S
Wölpl
A
A
Ballas
M
M
Goldmann
S F
SF
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D006684:HLA-DR Antigens / Q000302:isolation & purification; D059810:HLA-DR Serological Subtypes; D006650:Histocompatibility Testing; D006801:Humans; D007202:Indicators and Reagents; D007963:Leukocytes, Mononuclear / Q000276:immunology; D051379:Mice
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006684:HLA-DR Antigens; D059810:HLA-DR Serological Subtypes; C051104:HLA-DR52 antigen; D007202:Indicators and Reagents