title: Conversion of normal behavior to shiverer by myelin basic protein antisense cDNA in transgenic mice.
abstract: Myelin basic proteins (MBPs) are coded by the single gene necessary for myelin formation in the central nervous system of the mouse. An antisense MBP mini-gene was constructed and used to determine the function of antisense DNA in transgenic mice. Several transgenic offspring of a founder transgenic mouse, AS100, were converted from the normal to mutant shiverer phenotype. Antisense MBP messenger RNA was expressed in these mice, and the endogenous MBP messenger RNA, the MBP, and the myelination in the central nervous system were reduced.
authors: Department of DNA Biology, School of Medicine, Tokai University, Isehara, Japan.
authors: M
authors: M
authors: Katsuki
authors: M
authors: M
authors: Sato
authors: M
authors: M
authors: Kimura
authors: M
authors: M
authors: Yokoyama
authors: K
authors: K
authors: Kobayashi
authors: T
authors: T
authors: Nomura
mesh_terms: D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000502:physiology; D004247:DNA / Q000235:genetics; D005786:Gene Expression Regulation; D051379:Mice; D008818:Mice, Neurologic Mutants / Q000502:physiology*; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D004676:Myelin Basic Protein / Q000235:genetics / Q000502:physiology*; D009186:Myelin Sheath / Q000502:physiology; D010641:Phenotype; D012313:RNA / Q000235:genetics*; D016372:RNA, Antisense
chemical_list: D004676:Myelin Basic Protein; D016372:RNA, Antisense; D012313:RNA; D004247:DNA