title: Quantitation of HIV-1 activity in tissue culture supernatants: effects of culture condition on syncytial assays and virus production.
abstract: We have compared the infectivity titres, reverse transcriptase levels and antigen titres in the supernatants from persistently infected cell lines that produce a variety of HIV-1 isolates. We found a poor correlation between the different assays, and a variability in viral activity dependent on culture medium.
authors: Department of Immunological Medicine, Clinical Research Centre, Harrow, Oxford, U.K.
authors: G
authors: G
authors: Spickett
authors: R E
authors: RE
authors: Beattie
authors: L
authors: L
authors: Bountiff
authors: A G
authors: AG
authors: Dalgleish
authors: A D
authors: AD
authors: Webster
mesh_terms: D002459:Cell Fusion; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D004795:Enzyme Stability; D015488:HIV Antigens / Q000032:analysis; D015497:HIV-1 / Q000254:growth & development / Q000502:physiology*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013045:Species Specificity; D014779:Virus Replication
chemical_list: D003470:Culture Media; D015488:HIV Antigens; D012194:RNA-Directed DNA Polymerase