title: Class I major histocompatibility proteins as cell surface receptors for simian virus 40.
abstract: Class I major histocompatibility complex proteins appear to be the major cell surface receptors for simian virus 40 (SV40), as implied by the following observations. Adsorption of SV40 to LLC-MK2 rhesus monkey kidney cells specifically inhibited binding of a monoclonal antibody (MAb) against class I human lymphocyte antigen (HLA) proteins. Conversely, pretreatment of LLC-MK2 cells with anti-HLA MAbs inhibited infection by SV40. The ability of anti-HLA to inhibit infection was greatly reduced when the order of addition of the anti-HLA and the virus was reversed. Infection was also inhibited by preincubating SV40 with purified soluble class I protein. Finally, human lymphoblastoid cells of the Daudi line, which do not express class I major histocompatibility complex proteins, were infected at relatively low levels with SV40 virions. In a control experiment, we found that pretreatment of cells with a MAb specific for the leukocytic-function-associated antigen LFA-3 actually enhanced infection. This finding may also support the premise that class I major histocompatibility complex proteins are receptors for SV40.
authors: Graduate Program in Neuroscience and Behavior, University of Massachusetts, Amhers 01003.
authors: W J
authors: WJ
authors: Atwood
authors: L C
authors: LC
authors: Norkin
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000502:physiology; D018968:CD58 Antigens; D015395:Histocompatibility Antigens Class I / Q000276:immunology / Q000502:physiology*; D006801:Humans; D007136:Immunoglobulins / Q000502:physiology; D008562:Membrane Glycoproteins / Q000502:physiology; D011991:Receptors, Virus / Q000502:physiology*; D013539:Simian virus 40 / Q000472:pathogenicity*
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D018968:CD58 Antigens; D015395:Histocompatibility Antigens Class I; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D011991:Receptors, Virus