Immunohistochemical study of uterine stromal sarcoma and rhabdomyosarcoma.
The cytoplasmic filament composition of two pure high-grade endometrial stromal sarcomas and five pure endometrial rhabdomyosarcomas was studied using the immunoperoxidase avidin-biotin method. Ultrastructural correlates were performed on the same tissues. While four of the seven tumors presented as high-grade undifferentiated neoplasms, they were readily categorized on the basis of immunocytochemical findings in which the five rhabdomyosarcomas were positive for muscle-specific actin (HHF 35) and the two stromal sarcomas were positive for vimentin only. Ultrastructure on the HHF 35-positive cases showed the presence of thick filaments and Z-band material, whereas the other tumors showed no cytoplasmic differentiation. Muscle-type actin differs stromal cells, and is useful when used in conjunction with a panel of antibodies in the categorization of undifferentiated endometrial sarcomas.
Dabbs
D J
DJ
Case Western Reserve University School of Medicine, Cleveland (Ohio) Metropolitan General Hospital.
Silverman
J F
JF
Geisinger
K R
KR
D014408:Biomarkers, Tumor / Q000032:analysis; D003598:Cytoskeletal Proteins / Q000032:analysis*; D003893:Desmin / Q000032:analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis; D008875:Middle Aged; D009211:Myoglobin / Q000032:analysis; D012208:Rhabdomyosarcoma / Q000032:analysis / Q000473:pathology*; D012509:Sarcoma / Q000032:analysis / Q000473:pathology*; D014594:Uterine Neoplasms / Q000032:analysis / Q000473:pathology*; D014746:Vimentin / Q000032:analysis
D014408:Biomarkers, Tumor; D003598:Cytoskeletal Proteins; D003893:Desmin; D009211:Myoglobin; D014746:Vimentin; D007633:Keratins