title: Characterization of two lignin peroxidase clones from Phanerochaete chrysosporium.
abstract: Two cDNA clones encoding lignin peroxidase isozymes from Phanerochaete chrysosporium have been isolated and characterized. One of the clones, lambda ML-4, encodes isozyme H8 as does the previously reported clone lambda ML-1 [Tien, M. and Tu, C.-P.D. Nature 326 (1987) 520-523; 328, 742]. Our data are consistent with lambda ML-1 and lambda ML-4 being allelic variants. The other clone, lambda ML-5, encodes a homologous isozyme. We have also isolated the genomic clone corresponding to lambda ML-4 cDNA. Conserved residues thought to be essential for peroxidase function were identified in the predicted amino acid sequences of both cDNA clones. Northern blot analyses indicate that these isozymes are expressed during secondary metabolism, appearing on day 4 of growth and increasing on days 5 and 6.
authors: Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.
authors: A
authors: A
authors: Andrawis
authors: E A
authors: EA
authors: Pease
authors: I C
authors: IC
authors: Kuan
authors: E
authors: E
authors: Holzbaur
authors: M
authors: M
authors: Tien
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D005658:Fungi / Q000201:enzymology*; D006031:Glycosylation; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D010544:Peroxidases / Q000235:genetics*; D012313:RNA / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid
chemical_list: D007527:Isoenzymes; D015345:Oligonucleotide Probes; D012313:RNA; D004247:DNA; D010544:Peroxidases; C042858:lignin peroxidase; D004262:DNA Restriction Enzymes