Identification of a site that mediates transcriptional response of the human beta-actin gene to serum factors.
In quiescent fibroblasts, low-level transcription of the beta-actin gene occurs, and it is rapidly induced by growth factors in serum. Deletion analysis of the 5' upstream region of the human beta-actin gene using a CAT assay showed that the region between +765 and +783 downstream from the cap site, which had enhancer activity in growing NIH3T3 cells, also acted as a serum-response element in quiescent cells. The data suggest that the 19-bp element acts positively as a serum response element. This sequence contains a CC(A/T)6GG sequence which is found in the promoter region of many cytoskeletal and muscle-specific actin genes.
Orita
S
S
Department of Oncogene Research, Osaka University, Japan.
Makino
K
K
Kawamoto
T
T
Niwa
H
H
Sugiyama
H
H
Kakunaga
T
T
D000199:Actins / Q000235:genetics*; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D006133:Growth Substances / Q000097:blood / Q000502:physiology*; D008969:Molecular Sequence Data; D010957:Plasmids; D012313:RNA / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014158:Transcription, Genetic; D014162:Transfection
D000199:Actins; D006133:Growth Substances; D012313:RNA; D015500:Chloramphenicol O-Acetyltransferase