Sequence and expression of mRNAs encoding the alpha 1 and alpha 2 subunits of a DHP-sensitive calcium channel.
Complementary DNAs were isolated and used to deduce the primary structures of the alpha 1 and alpha 2 subunits of the dihydropyridine-sensitive, voltage-dependent calcium channel from rabbit skeletal muscle. The alpha 1 subunit, which contains putative binding sites for calcium antagonists, is a hydrophobic protein with a sequence that is consistent with multiple transmembrane domains and shows structural and sequence homology with other voltage-dependent ion channels. In contrast, the alpha 2 subunit is a hydrophilic protein without homology to other known protein sequences. Nucleic acid hybridization studies suggest that the alpha 1 and alpha 2 subunit mRNAs are expressed differentially in a tissue-specific manner and that there is a family of genes encoding additional calcium channel subtypes.
Ellis
S B
SB
Salk Institute Biotechnology/Industrial Associates, Inc., La Jolla, CA 92037.
Williams
M E
ME
Ways
N R
NR
Brenner
R
R
Sharp
A H
AH
Leung
A T
AT
Campbell
K P
KP
McKenna
E
E
Koch
W J
WJ
Hui
A
A
Schwartz
A
A
Harpold
M M
MM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D003001:Cloning, Molecular; D004247:DNA; D004262:DNA Restriction Enzymes; D004095:Dihydropyridines / Q000494:pharmacology; D007473:Ion Channels / Q000187:drug effects; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010449:Peptide Mapping; D012333:RNA, Messenger / Q000096:biosynthesis; D011817:Rabbits; D012689:Sequence Homology, Nucleic Acid
D002121:Calcium Channel Blockers; D004095:Dihydropyridines; D007473:Ion Channels; D012333:RNA, Messenger; D004247:DNA; D004262:DNA Restriction Enzymes; D002118:Calcium