Chromatographic purification of gamma-globulin from human serum on ferric oxide-agarose columns.
A fast chromatographic procedure for the purification of the gamma-globulin fraction of human serum on ferric oxide-containing agarose has been developed. The presence of ferric oxide (to which gamma-globulin is absorbed) increases the rigidity of the beads. They can therefore be used at high flow rates without troublesome crosslinking. The separation has been performed on a 14-ml column and on a 1.5-ml disposable column. The yield is 87 +/- 6% and the gamma-globulin fraction is homogeneous in agarose gel electrophoresis.
Hjertén
S
S
Institute of Biochemistry, Biomedical Center, University of Uppsala, Sweden.
Zelikman
I
I
D002847:Chromatography, Agarose; D005290:Ferric Compounds; D006801:Humans; D005719:gamma-Globulins / Q000302:isolation & purification*
D005290:Ferric Compounds; D005719:gamma-Globulins; C000499:ferric oxide