Description of an endometrioid ovarian cancer cell line.
A human cell line, designated L-1, has been established from the ascites of an untreated patient with stage IV (FIGO) endometrioid ovarian cancer. This cell line initially grew uninterupted for 6 months without fibroblast contamination and contact inhibition, and has been subcultured weekly for the past 7 years. L-1 does not contain steroid hormone receptors nor does it demonstrate the presence of oncofetal antigens by immunohistochemical techniques. The doubling time of L-1 is 11.8 hr. Flow cytometric analysis reveals an aneuploid DNA peak, and an abnormal karyotype demonstrates hyperdiploidy, translocations, and deletions. Morphology, growth patterns, cytogenetic analysis, and other features of L-1 are characterized.
Giancotti
F R
FR
Department of Obstetrics and Gynecology, Lenox Hill Hospital, New York, New York.
Dorsett
B H
BH
Weaver
S C
SC
Bharathur
R
R
Ioachim
H L
HL
Barber
H R
HR
D002272:Carcinoembryonic Antigen / Q000378:metabolism; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D002875:Chromosomes / Q000648:ultrastructure; D004715:Endometriosis / Q000378:metabolism / Q000473:pathology*; D005260:Female; D005434:Flow Cytometry; D006003:Glycogen / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D008875:Middle Aged; D009077:Mucins / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D011960:Receptors, Estrogen / Q000378:metabolism; D011980:Receptors, Progesterone / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D000509:alpha-Fetoproteins / Q000378:metabolism
D002272:Carcinoembryonic Antigen; D009077:Mucins; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D000509:alpha-Fetoproteins; D006003:Glycogen