Agonist-induced hydrolysis of phosphoinositides and formation of 1,2-diacylglycerol in adult human keratinocytes.
The ability of a variety of agonists to induce formation of inositol phosphates and 1,2-diacylglycerol in cultured adult human keratinocytes has been investigated. Histamine, bradykinin, and thrombin significantly stimulated formation of inositol mono-, bis-, and trisphosphate and 1,2-diacylglycerol within 5 min after addition. Aluminum fluoride also caused a dose-dependent accumulation of inositol phosphates suggesting the participation of a GTP binding protein in the regulation of phospholipase C-catalyzed phosphoinositide hydrolysis. These data demonstrate that human keratinocytes possess the capacity for phospholipase C-mediated signal transduction and suggest that this pathway may participate in the regulation of keratinocyte function.
Talwar
H S
HS
Department of Dermatology, University of Michigan Medical School, Ann Arbor 48109.
Fisher
G J
GJ
Harris
V A
VA
Voorhees
J J
JJ
D000535:Aluminum / Q000494:pharmacology; D017607:Aluminum Compounds; D001790:Blood Physiological Phenomena; D002118:Calcium / Q000494:pharmacology; D002478:Cells, Cultured; D004075:Diglycerides / Q000096:biosynthesis*; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism*; D005459:Fluorides / Q000494:pharmacology; D005989:Glycerides / Q000096:biosynthesis*; D006801:Humans; D006868:Hydrolysis; D007294:Inositol / Q000378:metabolism; D007295:Inositol Phosphates / Q000096:biosynthesis; D007633:Keratins; D010716:Phosphatidylinositols / Q000378:metabolism*; D010743:Phospholipids / Q000096:biosynthesis; D013268:Stimulation, Chemical
C011439:1,2-diacylglycerol; D017607:Aluminum Compounds; D004075:Diglycerides; D005989:Glycerides; D007295:Inositol Phosphates; D010716:Phosphatidylinositols; D010743:Phospholipids; D007294:Inositol; D007633:Keratins; D000535:Aluminum; D005459:Fluorides; D002118:Calcium; C032311:aluminum fluoride