title: Presence of phospho-tyrosine in alkaline hydrolysate of pig skeletal muscle myosin.
abstract: A small part of the P-containing fraction of myosin hydrolysate which could be eluted with basic solvent (triethylamine) buffer was accumulated on chromatographic column. On the basis of the elution profile, P- and tyrosine content determination, moreover the thin layer chromatographic separation and specific Tyr reaction, this fraction is suggested to be phosphotyrosine. The concentration of P-Tyr is about 2 mol in pig muscle myosin calculated for 500 kDa protein. As the higher P-containing myosins also have an approx. 2 mol P-Tyr, it is thought that this concentration had been present in myosins already before alkaline hydrolysis.
authors: Second Institute of Biochemistry, Semmelweis University Medical School, Budapest, Hungary.
authors: S
authors: S
authors: Fazekas
authors: I
authors: I
authors: Ováry
authors: V
authors: V
authors: Székessy-Hermann
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009132:Muscles / Q000032:analysis*; D009218:Myosins / Q000032:analysis*; D019000:Phosphotyrosine; D013552:Swine; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D019000:Phosphotyrosine; D014443:Tyrosine; D009218:Myosins