Semple rabies vaccine: presence of myelin basic protein and proteolipid protein and its activity in experimental allergic encephalomyelitis.
Myelin basic protein (MBP) as a cause of postvaccinal encephalomyelitis (PVE) due to Semple rabies vaccine (SRV) has been suggested in previous reports. No actual measurement of MBP in SRV was done. In this study we detected MBP and PLP in the vaccine using immunological methods. The vaccine was found to contain 28 micrograms MBP per ml vaccine. Inoculation with SRV plus adjuvant resulted in the development of experimental allergic encephalomyelitis (EAE) in 2 of 3 guinea pigs. For control, chick embryo vaccine (CEV) was used and MBP was not detected. EAE was not induced in animals inoculated with it. These results suggest that MBP in vaccines may play a decisive role in the production of PVE.
Javier
R S
RS
Division of Demyelinating Disease and Aging, National Institute of Neuroscience, NCNP, Tokyo, Japan.
Kunishita
T
T
Koike
F
F
Tabira
T
T
D000818:Animals; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D004676:Myelin Basic Protein / Q000032:analysis*; D009185:Myelin Proteins / Q000032:analysis*; D018991:Myelin Proteolipid Protein; D011819:Rabies Vaccines / Q000032:analysis*
D004676:Myelin Basic Protein; D009185:Myelin Proteins; D018991:Myelin Proteolipid Protein; D011819:Rabies Vaccines