Human autoantibody secreted by immortalized lymphocyte cell line against the 68K polypeptide of the U1 small nuclear ribonucleoprotein.
Autoantibody of an immortalized human lymphocyte cell line, Su-2E4, derived from peripheral lymphocytes of a patient with mixed connective tissue disease, showed specific binding of the 68K polypeptide of U1 small nuclear RNP (snRNP) and immunoprecipitation of U1 RNA. The reaction patterns of Su-2E4 and a murine monoclonal anti-(U1)snRNP line, 2.73, and results of a competition assay with the 2 antibodies suggest similar, but not necessarily identical, epitope recognition.
Chen
J T
JT
Dalton Research Center, University of Missouri, Columbia 65211.
Takeda
Y
Y
Vanderslice
W E
WE
Sharp
G C
GC
Pettersson
I
I
Rosén
A
A
Wigzell
H
H
Wang
R J
RJ
D001323:Autoantibodies / Q000032:analysis* / Q000276:immunology; D001667:Binding, Competitive; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015151:Immunoblotting; D008214:Lymphocytes / Q000276:immunology*; D008947:Mixed Connective Tissue Disease / Q000276:immunology; D008970:Molecular Weight; D010455:Peptides / Q000276:immunology*; D011233:Precipitin Tests; D012313:RNA / Q000032:analysis; D012261:Ribonucleoproteins / Q000235:genetics / Q000276:immunology*; D017411:Ribonucleoproteins, Small Nuclear
D001323:Autoantibodies; D010455:Peptides; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear; D012313:RNA