The unique specificity of antibodies in modern radioimmunochemistry--an essay on assays.
The gradual recognition and exploitation of the specificity of antibodies in peptide radioimmunoassays (RIA) during the last three decades are reviewed. From the old fashioned RIA techniques of the sixties through sequence-specific RIA libraries of the seventies to residue-specific immunoassays of the eighties, the RIA technique has to an increasing degree been based on the ability to select strictly monospecific antibodies in high-titered polyclonal antisera. High-avidity monospecific antibodies from polyclonal antisera provide the RIA technology of to-day with unrivalled specificity. This specificity again has increased the utility of RIA as a tool in basic as well as clinical biochemistry.
Rehfeld
J F
JF
Department of Clinical Chemistry, Rigshospitalet, University of Copenhagen, Denmark.
Bardram
L
L
Cantor
P
P
Hilsted
L
L
Johnsen
A H
AH
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D000939:Epitopes; D049673:History, 20th Century; D006801:Humans; D010455:Peptides / Q000276:immunology; D011863:Radioimmunoassay / Q000266:history / Q000379:methods*
D000939:Epitopes; D010455:Peptides