RNA in the nucleus of a motile plant spermatozoid: characterization by enzyme-gold cytochemistry and in situ hybridization.
To investigate the decrease of transcription and to determine the localization of RNA molecules during spermiogenesis in the fern Scolopendrium, we have used the enzyme-gold electron microscope method. During cell differentiation, the labeling decreases over the cytoplasm and increases over the nucleus. In the latter, the nucleolus disappears at the beginning of differentiation and the gold particles that were at first situated over the dispersed chromatin are then located over the condensed chromatin. In mature gametes, gold particles linked to RNA are abundant over the dense and homogeneous nucleus and sparse over the cytoplasm. By using in situ hybridization of RNA-poly(A) tails with radioactive poly(U) probes, we have shown that these RNA molecules are mRNA; they are located in the condensed chromatin of the mature sperm nucleus.
Rejon
E
E
Biologie de la Reproduction des Végétaux, Université P. & M. Curie, Paris, France.
Bajon
C
C
Blaize
A
A
Robert
D
D
D001345:Autoradiography; D002467:Cell Nucleus / Q000378:metabolism*; D006046:Gold; D006651:Histocytochemistry; D008297:Male; D009693:Nucleic Acid Hybridization; D018521:Plant Physiological Phenomena; D010944:Plants / Q000235:genetics; D012313:RNA / Q000235:genetics / Q000378:metabolism*; D012259:Ribonuclease, Pancreatic; D013091:Spermatogenesis / Q000502:physiology; D014158:Transcription, Genetic
D012313:RNA; D006046:Gold; D012259:Ribonuclease, Pancreatic