Inhibitory effect of human recombinant interferon gamma on synthesis of acute phase proteins in human hepatoma Hep G2 cells stimulated by leukocyte cytokines, TNF alpha and IFN-beta 2/BSF-2/IL-6.
Supernatants from endotoxin-stimulated human leukemic cells and human recombinant interferon-beta 2 similarly enhance synthesis of alpha 1-antichymotrypsin and haptoglobin but suppress synthesis of albumin in cultured Hep G2 cells. Human recombinant tumor necrosis factor only slightly affects production of alpha 1-antichymotrypsin and albumin in a similar manner as leukocyte cytokines. In distinction, recombinant human interferon-gamma profoundly inhibits synthesis of alpha 1-antichymotrypsin, and especially of haptoglobin, but stimulates production of alpha 2-macroglobulin thus modulating the acute phase response of these cells.
Magielska-Zero
D
D
Institute of Molecular Biology, Jagiellonian University, Krakow, Poland.
Bereta
J
J
Czuba-Pelech
B
B
Pajdak
W
W
Gauldie
J
J
Koj
A
A
D000209:Acute-Phase Proteins / Q000096:biosynthesis*; D000418:Albumins / Q000096:biosynthesis; D001685:Biological Factors / Q000494:pharmacology; D001798:Blood Proteins / Q000096:biosynthesis; D006528:Carcinoma, Hepatocellular / Q000378:metabolism; D016207:Cytokines; D005340:Fibrinogen / Q000096:biosynthesis; D006242:Haptoglobins / Q000096:biosynthesis; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology*; D008113:Liver Neoplasms; D009363:Neoplasm Proteins / Q000096:biosynthesis; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D000514:alpha 1-Antichymotrypsin / Q000096:biosynthesis; D000515:alpha 1-Antitrypsin; D000511:alpha-Macroglobulins / Q000096:biosynthesis
D000209:Acute-Phase Proteins; D000418:Albumins; D001685:Biological Factors; D001798:Blood Proteins; D016207:Cytokines; D006242:Haptoglobins; D007370:Interferon Type I; D009363:Neoplasm Proteins; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D000514:alpha 1-Antichymotrypsin; D000515:alpha 1-Antitrypsin; D000511:alpha-Macroglobulins; D007371:Interferon-gamma; D005340:Fibrinogen