Polyomavirus transforms rat F111 and mouse NIH 3T3 cells by different mechanisms.
Polyomavirus middle tumor antigen (mT) was expressed in a line of mouse NIH 3T3 cells under control of the dexamethasone-regulatable mouse mammary tumor virus promotor. Contrary to rat F111 cells which were rendered anchorage independent by mT expression alone (L. Raptis, H. Lamfrom, and T.L. Benjamin, Mol. Cell. Biol. 5:2476-2487, 1985), mT-producing NIH 3T3 cells were unable to grow in agar even after full mT induction. The mT:pp60c-src-associated phosphatidylinositol kinase was activated in these cells to a degree similar to that in fully transformed cells expressing the small and large T antigens, in addition to mT. We therefore propose that the stimulation of this phosphatidylinositol kinase, although apparently necessary, is not sufficient for transformation of NIH 3T3 cells by polyomavirus.
Raptis
L
L
Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada.
Bolen
J B
JB
D019870:1-Phosphatidylinositol 4-Kinase; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000502:physiology*; D002460:Cell Line; D002472:Cell Transformation, Viral; D003907:Dexamethasone / Q000494:pharmacology; D005347:Fibroblasts; D005786:Gene Expression Regulation / Q000187:drug effects; D051379:Mice; D010770:Phosphotransferases / Q000502:physiology; D011120:Polyomavirus / Q000502:physiology*; D011401:Promoter Regions, Genetic / Q000187:drug effects; D011518:Proto-Oncogene Proteins / Q000502:physiology; D016392:Proto-Oncogene Proteins pp60(c-src); D051381:Rats; D011916:Rats, Inbred F344; D011993:Recombinant Fusion Proteins / Q000502:physiology; D013045:Species Specificity
D000952:Antigens, Polyomavirus Transforming; D011518:Proto-Oncogene Proteins; D011993:Recombinant Fusion Proteins; D003907:Dexamethasone; D010770:Phosphotransferases; D019870:1-Phosphatidylinositol 4-Kinase; D016392:Proto-Oncogene Proteins pp60(c-src)