title: Rescue effect of exogenous reduced folates on methotrexate polyglutamylation and dihydrofolate reductase activity in L1210 cells.
abstract: L1210 can be rescued from exposure to an inhibitory effect of methotrexate (MTX) by subsequent addition of 5-formyltetrahydrofolate, 5-methyltetrahydrofolate or dihydrofolate. All folates listed caused marked reduction of long-chain MTX polyglutamates content and increased the activity of dihydrofolate reductase. This indicates that the rescue is a result of interaction of the reduced folates with two processes-polyglutamylation of MTX and generation of dihydrofolate.
authors: Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, Polish Academy of Sciences.
authors: M
authors: M
authors: Balińska
mesh_terms: D000818:Animals; D005492:Folic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D005575:Formyltetrahydrofolates / Q000494:pharmacology*; D007939:Leukemia L1210 / Q000378:metabolism / Q000473:pathology; D008727:Methotrexate / Q000031:analogs & derivatives* / Q000378:metabolism / Q000633:toxicity*; D051379:Mice; D010455:Peptides / Q000378:metabolism*; D011099:Polyglutamic Acid / Q000031:analogs & derivatives / Q000378:metabolism*; D013762:Tetrahydrofolate Dehydrogenase / Q000378:metabolism*; D013763:Tetrahydrofolates / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D005575:Formyltetrahydrofolates; D010455:Peptides; D013763:Tetrahydrofolates; D011099:Polyglutamic Acid; C010920:dihydrofolate; C014085:methotrexate polyglutamate; D005492:Folic Acid; D013762:Tetrahydrofolate Dehydrogenase; C005984:5-methyltetrahydrofolate; D008727:Methotrexate