title: The optimum pH of serum alkaline phosphatase after induced cholestasis in the male rat, mouse and rabbit.
abstract: The optimum pH of serum alkaline phosphatase (alk. P., EC 3.1.3.1) was investigated in adult male rats, mice and rabbits after fasting or after the induction of cholestasis by a single oral dose of 200 mg/kg alpha-naphthylisothiocyanate (ANIT). The nature of serum alk.P. was also studied with the inhibitory effects of three L-amino acids (L-phenylalanine, L-homoarginine and L-cystine) and by electrophoresis. In the rat, fasting as well as ANIT-treatment shifted the optimum pH of alk.P. from 9.4 to 10.0 due to a decrease in intestinal isoenzymes or due to newly appeared liver isoenzymes, while no shifting occurred in mouse and rabbit.
authors: Department of Experimental Pathology, Nippon Boehringer Ingelheim Co., Ltd., Hyogo, Japan.
authors: T
authors: T
authors: Yabe
authors: A
authors: A
authors: Kast
mesh_terms: D015058:1-Naphthylisothiocyanate; D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000097:blood*; D000818:Animals; D002779:Cholestasis / Q000097:blood / Q000139:chemically induced / Q000201:enzymology*; D003553:Cystine / Q000494:pharmacology; D004588:Electrophoresis, Cellulose Acetate; D005215:Fasting; D006709:Homoarginine / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007422:Intestines / Q000201:enzymology; D007527:Isoenzymes / Q000097:blood; D008099:Liver / Q000201:enzymology; D008297:Male; D051379:Mice; D010649:Phenylalanine / Q000494:pharmacology; D011817:Rabbits; D051381:Rats
chemical_list: D007527:Isoenzymes; D006709:Homoarginine; D010649:Phenylalanine; D003553:Cystine; D015058:1-Naphthylisothiocyanate; D000469:Alkaline Phosphatase