Definition of DRw10 specificity by restriction fragment length polymorphism.
The purpose of this study was the RFLP characterization of the DRw10 specificity. Twenty-two DRw10 cells were tested: the DNAs were digested by seven restriction enzymes and hybridized with DR beta, DQ beta and DQ alpha probes. Hybridization with DR beta revealed a pattern particular to the DRw10 specificity, with a specific TaqI 12.5Kb fragment. Hybridization with both DQ-specific probes showed that DRw10 is always associated with a special DQw1 subtype: DQw5. Furthermore, at DR and DQ levels, the 22 DRw10 cells behaved homogeneously.
Semana
G
G
Laboratoire d'histocompatibilité, Centre Régional de Transfusion Sanguine de Rennes, France.
Bignon
J D
JD
Quillivic
F
F
Cheneau
M L
ML
Herniou
E
E
Muller
J Y
JY
Genetet
B
B
Fauchet
R
R
D000483:Alleles; D000939:Epitopes / Q000276:immunology; D008040:Genetic Linkage; D006683:HLA-DQ Antigens / Q000235:genetics; D006684:HLA-DR Antigens / Q000235:genetics* / Q000276:immunology; D015798:HLA-DR1 Antigen; D015802:HLA-DR2 Antigen; D015808:HLA-DR6 Antigen; D006239:Haplotypes; D006801:Humans; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D000939:Epitopes; D006683:HLA-DQ Antigens; C051568:HLA-DQ1 antigen; D006684:HLA-DR Antigens; D015798:HLA-DR1 Antigen; D015802:HLA-DR2 Antigen; D015808:HLA-DR6 Antigen; C043263:HLA-DRw10