Sulphate and methionine as sulphur sources for cysteine and cephalosporin C synthesis in Cephalosporium acremonium.
Three prototrophic strains of Cephalosporium acremonium with different potentials for cephalosporin C production show pronounced differences in pools of intracellular sulphur amino acids. The superior strain exhibits the largest pool, particularly of cysteine (four times that of the non-producing strain). In fermentations with this strain methionine was found to be a much better sulphur source than sulphate for both cysteine and antibiotic synthesis. In the presence of methionine the utilization of sulphate is strongly depressed.
Lewandowska
M
M
Biotechnology Research and Development Center, Warszawa, Poland.
Paszewski
A
A
D000164:Acremonium / Q000254:growth & development / Q000378:metabolism*; D002511:Cephalosporins / Q000096:biosynthesis*; D003470:Culture Media; D003545:Cysteine / Q000096:biosynthesis*; D008715:Methionine / Q000378:metabolism*; D013045:Species Specificity; D013431:Sulfates / Q000378:metabolism*; D013455:Sulfur / Q000378:metabolism*
D002511:Cephalosporins; D003470:Culture Media; D013431:Sulfates; C025163:cephalosporin C; D013455:Sulfur; D008715:Methionine; D003545:Cysteine