A recombinant autoantigen derived from the human (U1) small nuclear RNP-specific 68-kd protein. Expression in Escherichia coli and serodiagnostic application.
A human liver complementary DNA expression library was screened using sera from patients with high titers of autoantibodies, to search for clones expressing major autoantigens that are relevant in connective tissue diseases. One of the clones isolated expressed a major epitope(s) that was immunoreactive with anti-U1 RNP sera, as shown by several techniques. Affinity-purified autoantibodies from the cloned RNP protein specifically recognized the 68-kd U1 RNP protein of HeLa cell nuclear extracts. All sera containing anti-U1 RNP antibodies detected by immunodiffusion, counterimmunoelectrophoresis, or immunoblotting also recognized the cloned RNP protein. The RNP antigen-expressing bacterial colonies and the partially purified cloned RNP fusion protein have been applied to fast and sensitive immunologic assays for the detection and quantification of anti-U1 RNP antibodies.
Netter
H J
HJ
Max Planck Institut für Biochemie, Martinsried, Federal Republic of Germany.
Guldner
H H
HH
Szostecki
C
C
Lakomek
H J
HJ
Will
H
H
D001324:Autoantigens / Q000276:immunology*; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D004926:Escherichia coli / Q000276:immunology*; D006801:Humans; D008970:Molecular Weight; D011994:Recombinant Proteins / Q000276:immunology*; D012261:Ribonucleoproteins / Q000276:immunology*; D012698:Serologic Tests / Q000379:methods*
D001324:Autoantigens; D000939:Epitopes; D011994:Recombinant Proteins; D012261:Ribonucleoproteins