Quality control of 99mTc-labeled dextran for lymphoscintigraphy.
99mTc-labeled dextran has been suggested as a lymphoscintigraphic agent. However, quality-control results from previous studies have been controversial. In this study, the optimal concentration of stannous ion and pH value were determined to obtain maximal labeling. Paper and thin-layer chromatography showed total labeling efficiency as high as 98.4%. Anthrone test of the supernatant of the segments from thin-layer chromatographic strip was performed. Colorimetric determinations verified that dextran was found in the same locations as the peak radioactivity.
Yang
K T
KT
Department of Nuclear Medicine, Changhua Christian Hospital, Taiwan.
Jong
S B
SB
Wu
C C
CC
Chen
J Y
JY
Chen
Y J
YJ
Yang
L H
LH
D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D003911:Dextrans; D004355:Drug Stability; D006801:Humans; D061305:Lymphoscintigraphy; D009942:Organometallic Compounds; D015609:Organotechnetium Compounds; D011786:Quality Control; D013667:Technetium
D003911:Dextrans; D009942:Organometallic Compounds; D015609:Organotechnetium Compounds; C034146:technetium Tc 99m dextran; D013667:Technetium