Effect of amiloride on arachidonic acid and histamine release from rat mast cells.
The effect of a putative Na+/H+ exchange inhibition on histamine and [14C]arachidonic acid ([14C]AA) release has been examined in rat peritoneal mast cells, using either addition of amiloride or removal of extracellular Na+. The cells were stimulated by non-immunological agents, i.e. calcium ionophore A23187, nerve growth factor (NGF), thapsigargin and compound 48/80. On the basis of the results obtained, a possible role for Na+/H+ exchange in rat mast cell secretion is discussed.
Linnebjerg
H
H
Pharma Biotec Research Center, Royal Danish School of Pharmacy, Copenhagen, Denmark.
Hansen
H S
HS
Jensen
B
B
D000584:Amiloride / Q000494:pharmacology*; D000818:Animals; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism*; D006636:Histamine Release / Q000187:drug effects*; D066298:In Vitro Techniques; D007474:Ion Exchange; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D011522:Protons; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000378:metabolism
D001095:Arachidonic Acids; D011522:Protons; D016718:Arachidonic Acid; D000584:Amiloride; D012964:Sodium