Phosphoribosylpyrophosphate (PRPP)-less mutants of Escherichia coli.
A DNA fragment encoding kanamycin resistance was inserted in vitro into a plasmid-borne prs gene encoding phosphoribosylpyrophosphate synthetase of Escherichia coli. The resulting plasmids were subsequently transferred to the chromosome by homologous recombination and the haploid strains prs-3::KanR and prs-4::KanR were obtained. These strains were fully viable, but required guanosine, uridine, histidine, tryptophan and nicotinamide mononucleotide. There was no phosphoribosylpyrophosphate synthetase activity or phosphoribosylpyrophosphate pool in the mutant strains. These results show that phosphoribosylpyrophosphate synthetase is dispensable for E. coli.
Hove-Jensen
B
B
Enzyme Division, University Institute of Biological Chemistry B, Copenhagen, Denmark.
D000483:Alleles; D000582:Amidophosphoribosyltransferase / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D009154:Mutation; D010430:Pentosyltransferases / Q000235:genetics*; D010754:Phosphoribosyl Pyrophosphate / Q000235:genetics; D010957:Plasmids; D011995:Recombination, Genetic
D010754:Phosphoribosyl Pyrophosphate; D010430:Pentosyltransferases; D000582:Amidophosphoribosyltransferase