title: Shared surface epitopes among trypanosomes of the same serodeme expressing different variable surface glycoprotein genes.
abstract: African trypanosomes evade the immune response of the mammalian host by undergoing antigenic variation, caused by sequence changes in a variable surface glycoprotein (VSG). The majority of trypanosome clones analyzed thus far are not known to share surface exposed epitopes or express appreciably homologous VSGs. We show here that four clones of Trypanosoma brucei from the same serodeme express different VSGs and share exposed epitopes to varying degrees, as defined by monoclonal antibodies. Rabbit antiserum against any one of the four VSGs recognizes epitopes present on all four trypanosomes in live cell immunofluorescence assay. The expressed VSGs are partially homologous at the N-terminus with multiple point substitutions of amino acids which distinguish each of the four VSGs. The genes coding for these VSGs are members of one gene family and an expression-linked copy with a unique restriction map is present in each trypanosome. Analysis of the ontogeny of the expressed genes should reveal mechanisms of evolution in trypanosome variable antigen repertoires.
authors: Department of Veterinary Microbiology and Pathology, Washington State University, Pullman.
authors: A F
authors: AF
authors: Barbet
authors: P J
authors: PJ
authors: Myler
authors: R O
authors: RO
authors: Williams
authors: T C
authors: TC
authors: McGuire
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000940:Antigenic Variation; D015342:DNA Probes; D000939:Epitopes / Q000235:genetics*; D015183:Restriction Mapping; D014348:Trypanosoma congolense / Q000145:classification / Q000235:genetics*; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000235:genetics*
chemical_list: D015342:DNA Probes; D000939:Epitopes; D014643:Variant Surface Glycoproteins, Trypanosoma