Mouse keratin 19: complete amino acid sequence and gene expression during development.
The complete amino acid sequence of the mouse keratin 19 (K19) was determined from a partial sequence of cDNA isolated from a mouse (day 10.5) embryo library and an amplified genomic fragment. Analysis of the sequence reveals strong evolutionary conservation with other K19s. Examination of the expression of the gene encoding K19 (K19) during development using an RNase protection assay reveals it is expressed in extra-embryonic tissues by day 8.5 and in the embryo proper by at least day 9.5. Furthermore, the K19 gene is induced in differentiating F9 embryonal carcinoma cells. These results indicate that K19 is another keratin, in addition to the K8-K18 pair, which is synthesized early during mouse development. Finally, Southern analysis of the K19 gene reveals that it is found as a unique copy in the mouse genome, in contrast to what is found in humans, which have at least one processed pseudogene.
Lussier
M
M
Institut du Cancer de Montréal, Québec, Canada.
Ouellet
T
T
Lampron
C
C
Lapointe
L
L
Royal
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004622:Embryo, Mammalian; D005314:Embryonic and Fetal Development; D005784:Gene Amplification; D015870:Gene Expression; D015723:Gene Library; D005796:Genes; D007633:Keratins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D012867:Skin / Q000378:metabolism
D012333:RNA, Messenger; D007633:Keratins