Concanavalin A reactivity of vitellogenin and yolk proteins of the threespined stickleback Gasterosteus aculeatus (Teleostei).
1. Concanavalin A (con A) reactive proteins have been detected in the plasma and ovaries of the oestradiol treated Gasterosteus aculeatus. 2. Concanavalin A-horseradish peroxidase (HRP) technique applied on nitrocellulose membranes reveals that vitellogenin (Vg) is the only mannose and glucose rich glycoprotein present in the plasma of oestradiol treated sticklebacks. Stickleback Vg can be purified by con A-Sepharose chromatography. 3. Con A reactivity in the ovary changes in the course of development of the oocytes. First, the yolk vesicles, which are synthesized by the oocyte itself, become con A positive. Later, the yolk granules, which contain vitellogenin synthesized in the liver and taken up from the plasma, show a clear affinity for con A. Con A staining disappears when mannopyranoside is added. 4. No con A staining is found in the periodic acid/Schiff staining chorion.
Covens
M
M
Zoological Institute, Louvain, Belgium.
Stynen
D
D
Ollevier
F
F
De Loof
A
A
D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification / Q000378:metabolism; D002846:Chromatography, Affinity; D003208:Concanavalin A / Q000378:metabolism*; D004527:Egg Proteins / Q000302:isolation & purification / Q000378:metabolism*; D004530:Egg Yolk; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005399:Fishes / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism; D010053:Ovary / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013194:Staining and Labeling; D014819:Vitellogenins / Q000378:metabolism*
D001798:Blood Proteins; D004527:Egg Proteins; D006023:Glycoproteins; D014819:Vitellogenins; D003208:Concanavalin A; D004958:Estradiol