Analysis of the structure and expression of the c-myc oncogene in cervical tumor and in cervical tumor-derived cell lines.
The structure of the c-myc oncogene in 17 cervical tumors and patient-matched nontumor tissues from Chinese patients residing in Taiwan was analysed. In contrast to recent reports on Mexican patients, none of the samples showed rearrangements and sequence amplification in the c-myc gene. The discrepancy may be explained by different carcinogenesis mechanisms being in operation in different geographic regions. Although no structural alterations in the c-myc gene were found in seven cervical carcinoma cell lines analysed, Northern blot analysis indicated different levels of c-myc gene expression which may be related to the presence of human papillomavirus (HPV) sequence in the cell and suggests a possible c-myc-hpv interaction in some stages of the transformation process.
Choo
K B
KB
Department of Medical Research, Veterans, General Hospital, Taipei, Taiwan, Republic of China.
Chong
K Y
KY
Chou
H F
HF
Liew
L N
LN
Liou
C C
CC
D015152:Blotting, Northern; D015139:Blotting, Southern; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002460:Cell Line; D004247:DNA / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D009693:Nucleic Acid Hybridization; D011518:Proto-Oncogene Proteins / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc; D011519:Proto-Oncogenes; D012313:RNA / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D012016:Reference Values; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
D004273:DNA, Neoplasm; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D012334:RNA, Neoplasm; D012313:RNA; D004247:DNA