[Immunochemical properties of immunoglobulin G conjugated with dextran].
Antigen-binding activity and effector functions of immunoglobulin G from horse and rabbit sera have been investigated, using hemagglutination, kinetic immune lysis, immune lysis in microplates and rosette-forming test with peritoneal mononuclear cells of mice, after their conjugation with dextran, MW 35-50 kD. The formation of conjugates of two types has been demonstrated: protein-dextran and protein-dextran-protein. It has been revealed that protein-dextran-protein conjugates have high specific antigen-binding activity, as compared to native IgG from rabbit sera specific for SRBC, while interactions with the complement system and Fc receptors is depressed.
Ivanov
A A
AA
Kalinin
N L
NL
Gromakovskaia
E T
ET
Levin
A D
AD
Podrez
E A
EA
D000818:Animals; D001666:Binding Sites, Antibody; D003165:Complement System Proteins / Q000276:immunology; D003911:Dextrans / Q000276:immunology*; D006386:Hemagglutination Tests; D006736:Horses / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D012397:Rosette Formation
D003911:Dextrans; D007074:Immunoglobulin G; C056869:immunoglobulin G-dextran conjugate; D003165:Complement System Proteins
Immunokhimicheskie svoĭstva immunoglobulina G, kon"iugirovannogo s dekstranom.