Localization of fucosyl residues in cellular compartments of rat duodenal absorptive enterocytes and goblet cells.
We have determined the subcellular distribution of fucosyl residues in rat duodenal absorptive enterocytes and goblet cells, using the binding affinity of the lectin I of Ulex europaeus (UEA I). In absorptive enterocytes, UEA I-lectin gold complexes were detected at the brush border and at the basolateral plasma membrane; pits of the plasma membrane were labeled, as were small vesicles, multivesicular bodies, lysosomes, and the Golgi apparatus. In the Golgi stacks, about half of the cisternae showed gold marker particles: accessible fucosyl residues were sparse in the cis subcompartment, the cismost cisterna mostly remaining negative; more intense label was found in medial cisternae; reactions were concentrated in the trans and transmost Golgi subcompartments. Cisternae, tubules and vesicles located at the trans Golgi side were the most constantly and intensely stained Golgi elements. In goblet cells, mucin granules and trans Golgi cisternae were labeled. Rarely, UEA I-gold bound to cisternae of the medial subcompartment; the cis subcompartment remained unstained. In part, UEA I-gold particles were restricted to dilated portions of the transmost Golgi cisterna and to secretory granules.
Ellinger
A
A
Institute of Micromorphology and Electron Microscopy, University of Vienna/Austria.
Pavelka
M
M
D000818:Animals; D004350:Drug Residues / Q000032:analysis*; D004386:Duodenum / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D005643:Fucose / Q000032:analysis*; D006056:Golgi Apparatus / Q000378:metabolism / Q000648:ultrastructure; D007150:Immunohistochemistry; D007408:Intestinal Absorption; D037102:Lectins / Q000378:metabolism; D008854:Microscopy, Electron; D037121:Plant Lectins; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling / Q000379:methods
D037102:Lectins; D037121:Plant Lectins; C036850:Ulex europaeus lectins; D005643:Fucose