Monoclonal antibodies against discoidin I and discoidin II of the cellular slime mold, Dictyostelium discoideum.
The preparation and properties of monoclonal antibodies against carbohydrate-binding proteins (discoidin I and discoidin II) in the cellular slime mold, Dictyostelium discoideum are described. Monoclonal antibody (mAb) ndI,II-1 bound both discoidins I and II specifically. mAb nI-1 and mAb dI-1 bound only discoidin I but their binding specificities were different: nI-1 recognized the native form and dI-1 the denatured form. mAb dII-1 bound only denatured discoidin II. In preliminary work mAbs dII-1 and nI-1 were found to be useful for localizing discoidins I and II immunohistochemically.
Fukuzawa
M
M
Department of Botany, Faculty of Science, Hokkaido University.
Ochiai
H
H
D000911:Antibodies, Monoclonal / Q000276:immunology; D001667:Binding, Competitive; D004023:Dictyostelium / Q000032:analysis*; D000070821:Discoidins; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D005656:Fungal Proteins / Q000276:immunology* / Q000302:isolation & purification; D015151:Immunoblotting / Q000379:methods; D037102:Lectins; D015800:Protozoan Proteins
D000911:Antibodies, Monoclonal; D000070821:Discoidins; D000939:Epitopes; D005656:Fungal Proteins; D037102:Lectins; D015800:Protozoan Proteins