[Binding of monoclonal antibodies to cerebral cytoplasmic tetrodotoxin-sensitive protein to human lymphocytes].
To examine whether lymphocytes express antigenic determinants of brain cytoplasmic tetrodotoxin-sensitive protein (CTSP), anti-CTSP monoclonal antibody (Bab) binding to human peripheral blood lymphocytes was studied using ELISA assay. It was shown that the Mab bound human lymphocytes in a dose-dependent fashion. Halph-maximal binding of this Mab was significantly enhanced if test-cells were pretreated with concanavalin A or with mixed lymphocyte culture supernatant. Results are discussed from the point of view of the hypothesis that CTSP are metabolic precursors of membrane sodium channels.
Pinchuk
G V
GV
Pinchuk
L N
LN
D000911:Antibodies, Monoclonal / Q000276:immunology; D001921:Brain / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003593:Cytoplasm / Q000378:metabolism*; D000939:Epitopes; D006801:Humans; D007473:Ion Channels; D008214:Lymphocytes / Q000276:immunology*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D011485:Protein Binding; D012964:Sodium / Q000378:metabolism; D015222:Sodium Channels; D013779:Tetrodotoxin
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D000939:Epitopes; D007473:Ion Channels; D009419:Nerve Tissue Proteins; D015222:Sodium Channels; C023790:tetrodotoxin-binding protein; D003208:Concanavalin A; D013779:Tetrodotoxin; D012964:Sodium
Sviazyvanie monoklonal'nykh antitel k tsitoplazmaticheskomu tetrodotoksinchuvstvitel'nomu belku golovnogo mozga s limfotsitami cheloveka.