Antiserum to pregnancy-associated plasma protein A (PAPP-A) recognizes human haptoglobin.
The presence of pregnancy-associated plasma protein A (PAPP-A) in non-pregnancy serum has been questioned with reference to insufficient specificity of the antibodies raised against this glycoprotein in some departments and used in immunoassay. For convenience, but also because of this unclear situation, many laboratories now use the only commercially available anti-PAPP-A preparations in their assays. By analysing pregnancy and non-pregnancy serum with two-dimensional electrophoresis (2-DE) and Western blotting, we could demonstrate that this antiserum marketed by Dakopatts (Denmark) was capable of binding haptoglobin whereas our own (Dr P. Bischof) antibody preparation did not recognize haptoglobin.
Bueler
M R
MR
Department of Clinical Chemistry, University Hospital, Zurich, Switzerland.
Bersinger
N A
NA
D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006242:Haptoglobins / Q000276:immunology*; D006801:Humans; D007106:Immune Sera; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000276:immunology*; D011266:Pregnancy-Associated Plasma Protein-A / Q000032:analysis / Q000276:immunology*; D011933:Reagent Kits, Diagnostic
D000911:Antibodies, Monoclonal; D006242:Haptoglobins; D007106:Immune Sera; D011257:Pregnancy Proteins; D011933:Reagent Kits, Diagnostic; D011266:Pregnancy-Associated Plasma Protein-A