Chronic exposure to pertussis toxin alters muscarinic receptor-mediated regulation of cyclic AMP metabolism in neuroblastoma x glioma NG108-15 hybrid cells.
Chronic pertussis toxin treatment (5 days) of NG108-15 neuroblastoma X glioma hybrid cells had no significant effect on basal cyclic AMP levels whereas it effectively blocked the inhibitory action of acute (10 min) exposure of carbachol (10(-4)M) on intracellular cyclic AMP accumulation, stimulated by prostaglandin E1. This action of pertussis toxin was found to be long lasting: exposure of the cells to pertussis toxin (100 ng/ml) for only 24 h followed by a 5-day withdrawal period still was shown effective on day 7 in abolishing the inhibitory action of carbachol on prostaglandin E1-stimulated cyclic AMP production. Chronic exposure (5 days) of NG108-15 cells to carbachol (10(-5)M) causes an increase in basal cyclic AMP levels by 98%, and a desensitization of the muscarinic inhibition of cyclic AMP accumulation, assessed after a 24-h withdrawal period. When carbachol treatment is carried out in the presence of pertussis toxin (100 ng/ml) both of these effects of carbachol are abolished.
Westlind-Danielsson
A
A
Department of Biochemistry, University of Stockholm, Sweden.
Gillenius
P
P
Askelöf
P
P
Bartfai
T
T
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000527:Alprostadil / Q000494:pharmacology; D001285:Atropine / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D000242:Cyclic AMP / Q000096:biosynthesis*; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006822:Hybrid Cells / Q000378:metabolism*; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology; D037342:Pertussis Toxin; D011976:Receptors, Muscarinic / Q000187:drug effects / Q000502:physiology*; D014407:Tumor Cells, Cultured; D010566:Virulence Factors, Bordetella / Q000494:pharmacology*
D011976:Receptors, Muscarinic; D010566:Virulence Factors, Bordetella; D001285:Atropine; D002217:Carbachol; D000242:Cyclic AMP; D037342:Pertussis Toxin; D000527:Alprostadil; D015056:1-Methyl-3-isobutylxanthine