Specific killing of human melanoma cells with an efficient 10B-compound on monoclonal antibodies.
We previously established methods which have enabled us to target a sufficient number of 10B atoms on human melanoma cells to destroy them by thermal neutron irradiation. Monoclonal antibodies were here used as vector of 10B atoms on the target cell. Thermal neutrons require at least 10(9) 10B atoms to destroy the cell. In order to accumulate an adequate number of 10B atoms on target cells, our first approach was to make an effective compound that contains 12 atoms of 10B in a molecule. The second step was to conjugate the compound with an avidin molecule (10B12-avidin). One molecule of the 10B12-avidin carries about 30 atoms of 10B. This 10B12-avidin can be specifically targeted on human melanoma cells by biotinated monoclonal antibodies specific for the cells. Furthermore, the number of 10B atoms on target cells can be augmented by a hapten-antihapten monoclonal antibody system. The cultured human melanoma cells treated with these methods were damaged by thermal neutron irradiation. This is the first study that indicates thermal neutrons do injure target cells boronated by monoclonal antibodies.
Komura
A
A
Department of Dermatology, Special Institute of Cancer Neutron Capture Therapy, Kobe University, Japan.
Tokuhisa
T
T
Nakagawa
T
T
Sasase
A
A
Ichihashi
M
M
Ferrone
S
S
Mishima
Y
Y
D000911:Antibodies, Monoclonal / Q000276:immunology / Q000627:therapeutic use*; D001895:Boron / Q000494:pharmacology / Q000627:therapeutic use; D001896:Boron Compounds / Q000494:pharmacology / Q000627:therapeutic use*; D002470:Cell Survival / Q000187:drug effects; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007554:Isotopes; D008545:Melanoma / Q000188:drug therapy / Q000473:pathology* / Q000532:radiotherapy; D009502:Neutrons; D011878:Radiotherapy / Q000379:methods; D012878:Skin Neoplasms / Q000188:drug therapy / Q000473:pathology* / Q000532:radiotherapy; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects
D000911:Antibodies, Monoclonal; D001896:Boron Compounds; D000939:Epitopes; D007554:Isotopes; D001895:Boron