Inhibitory effect of cyclic AMP on phorbol ester-stimulated production of reactive oxygen metabolites in rat glomeruli.
Studies were conducted to investigate cross-talk between protein kinase C (PKC) and cyclic AMP (cAMP) pathways using rat glomeruli (Glm). Phorbol 12-myristate 13-acetate (PMA), a PKC activator, stimulated production of reactive oxygen metabolites (ROM) in Glm. Forskolin and dibutyryl cAMP (Bt2cAMP) inhibited production of ROM dose-dependently. In the presence of both Bt2cAMP and 3-isobutyl-1-methylxanthine (IBMX) an additive effect was observed. Forskolin at 10(-4) inhibited translocation of PKC from the cytosol to the membrane. These results demonstrate that cAMP-mediated inhibition can occur at a step distal to PKC activation.
Miyanoshita
A
A
Department of Pharmacology, Faculty of Medicine, University of Tokyo, Japan.
Takahashi
T
T
Endou
H
H
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology*; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000502:physiology*; D007678:Kidney Glomerulus / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008163:Luminescent Measurements; D008297:Male; D010100:Oxygen / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D013806:Theophylline / Q000031:analogs & derivatives*
D005576:Colforsin; D003994:Bucladesine; D013806:Theophylline; D000242:Cyclic AMP; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D010100:Oxygen; D015056:1-Methyl-3-isobutylxanthine