Evidence for the expression of a primitive intestinal-like alkaline phosphatase in the intestinal 407 cell line.
Intestinal-like alkaline phosphatase was found to be expressed in the intestinal 407 cell line. This enzyme was identified by use of monoclonal antibodies specific for human placental (H7 and HPMS-1) and intestinal alkaline phosphatase (2HIMS-1 and 2HIMS-3) separately. Purification of this isozyme by use of two different monoclonal antibody immunoaffinity chromatographies demonstrates a single protein band on SDS-polyacrylamide gel electrophoresis indicating that this enzyme is not formed as a heterodimer. The apparent monomer subunit molecular weight and the dimer molecular weight of this isozyme were determined to 70000 and 160000, respectively. The enzyme is a homodimer according to molecular weight determinations. Furthermore, this isozyme is neuraminidase sensitive and comparatively heat stable, properties also characteristic for the placental enzyme. Our data suggest that the intestinal-like alkaline phosphatase in the intestinal 407 cell line displays properties intermediate of the intestinal and placental isozymes which may reflect the existence and reexpression of a new primitive isozyme.
Hirano
K
K
Gifu Pharmaceutical University, Japan.
Kusano
T
T
Asai
K
K
Hayashi
K
K
Stigbrand
T
T
Iino
S
S
Millan
J L
JL
D000469:Alkaline Phosphatase / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex / Q000032:analysis; D002460:Cell Line; D000939:Epitopes / Q000032:analysis; D005260:Female; D006801:Humans; D007422:Intestines / Q000201:enzymology*; D007527:Isoenzymes / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D008099:Liver / Q000201:enzymology; D008970:Molecular Weight; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000939:Epitopes; D007527:Isoenzymes; D000469:Alkaline Phosphatase