Dome formation of keratin-containing agranular cells from rat anterior pituitary gland in vitro.
A certain kind of cell in the pituitary gland exhibited immunoreactive keratin and dome formations in vitro. We obtained epithelial cells, which were able to subculture, from the outgrowth of anterior pituitary organ cultures. These cells lacked hormone secretory granules and exhibited immunoreactive keratin. Furthermore, they produced dome formations or cystic structures in monolayer culture and under three-dimensional culture condition using type I collagen gel. Dome formation was stimulated by dibutyryl cyclic AMP (dbcAMP, 10(-3) to 10(-5) M). Their responsiveness to dbcAMP is similar to that of several other epithelial cells that possess transport functions in vivo and in vitro. Although the origin of our cultured cells is unknown, these cells formed dome formations that possessed transport function and were related to cystic structures in the pituitary gland in vivo.
Shimada
T
T
Department of Anatomy, Jikei University School of Medicine, Tokyo, Japan.
Nakamura
F
F
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D003593:Cytoplasm / Q000648:ultrastructure; D003896:Desmosomes / Q000648:ultrastructure; D004847:Epithelial Cells; D005260:Female; D007382:Intermediate Filaments / Q000648:ultrastructure; D007633:Keratins / Q000032:analysis; D008297:Male; D008854:Microscopy, Electron; D009924:Organ Culture Techniques; D010903:Pituitary Gland, Anterior / Q000032:analysis / Q000166:cytology* / Q000648:ultrastructure; D051381:Rats
D003994:Bucladesine; D007633:Keratins