title: Identification of amino acids relevant to three antigenic determinants on the fusion protein of Newcastle disease virus that are involved in fusion inhibition and neutralization.
abstract: Nucleotide sequence analysis of F protein antigenic variants of Newcastle disease virus mapped three distinct antigenic determinants to positions 343, 72, and 161 on the protein. The high fusion-inhibiting and neutralizing capacities of all of the monoclonal antibodies used for selection suggested close functional and structural relationships of the three positions with the fusion-inducing N-terminal region of the F1 subunit. The former two positions were located at the cysteine cluster domain near the C terminus of the F1 subunit and at the major hydrophilic domain in the F2 subunit, respectively, and both domains appeared to represent the major antigenic determinants of paramyxovirus F protein.
authors: Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Japan.
authors: T
authors: T
authors: Toyoda
authors: B
authors: B
authors: Gotoh
authors: T
authors: T
authors: Sakaguchi
authors: H
authors: H
authors: Kida
authors: Y
authors: Y
authors: Nagai
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D000998:Antiviral Agents; D000939:Epitopes; D008969:Molecular Sequence Data; D009522:Newcastle disease virus / Q000276:immunology*; D014760:Viral Fusion Proteins / Q000276:immunology*
chemical_list: D000596:Amino Acids; D000911:Antibodies, Monoclonal; D000998:Antiviral Agents; D000939:Epitopes; D014760:Viral Fusion Proteins