title: Identification of a maltose-inducible major outer membrane protein in Actinobacillus (Haemophilus) pleuropneumoniae.
abstract: The addition of maltose to the growth media of Actinobacillus pleuropneumonia (serotype 1) resulted in the induction of an outer membrane protein (OMP) with a molecular mass of 42 kDa. This protein had porin-like properties in that it was peptidoglycan-associated and was resistant to proteolysis by trypsin. A pleuropneumoniae expressing the 42 kDa OMP were unable to bind lambda phage. Similar proteins were also induced in A. pleuropneumoniae isolates representing serotypes 2 to 7 with the exception of serotype 4; however, not all isolates of any given serotype expressed a maltose-inducible OMP. Western immunoblotting using convalescent antiserae against the serotype 1 A. pleuropneumoniae indicated that the 42 kDa OMP was expressed in vivo and was cross-reactive with the maltose-inducible OMPs from other serotypes.
authors: Veterinary Infectious Disease Organization, Saskatoon, Saskatchewan, Canada.
authors: H G
authors: HG
authors: Deneer
authors: A A
authors: AA
authors: Potter
mesh_terms: D000188:Actinobacillus / Q000032:analysis* / Q000187:drug effects; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology; D010582:Bacteriophage lambda / Q000378:metabolism; D000939:Epitopes / Q000032:analysis; D008320:Maltose / Q000494:pharmacology*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D000939:Epitopes; D008320:Maltose