Ribozyme, antisense RNA, and antisense DNA inhibition of U7 small nuclear ribonucleoprotein-mediated histone pre-mRNA processing in vitro.
A comparative analysis of ribozyme, antisense RNA, and antisense DNA inhibitors of the in vitro small nuclear ribonucleoprotein U7-dependent histone pre-mRNA processing reaction was performed. RNA molecules complementary to the U7 sequence inhibited in vitro processing of histone pre-mRNA at a sixfold excess over U7. Single-stranded DNA complementary to the entire U7 sequence inhibited the reaction at a 60-fold excess over U7, while a short, 18-nucleotide DNA molecule complementary to the 5' end of U7 inhibited the processing reaction at a 600-fold excess. A targeted ribozyme was capable of specifically cleaving the U7 small nuclear ribonucleoprotein in a nuclear extract and inhibited the U7-dependent processing reaction, but in our in vitro system it required a 1,000-fold excess over U7 for complete inhibition of processing.
Cotten
M
M
Institute for Molecular Pathology, Vienna, Austria.
Schaffner
G
G
Birnstiel
M L
ML
D000818:Animals; D002474:Cell-Free System; D004247:DNA / Q000494:pharmacology; D016373:DNA, Antisense; D006657:Histones / Q000235:genetics; D051379:Mice; D012313:RNA / Q000494:pharmacology; D012322:RNA Precursors / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional / Q000187:drug effects / Q000502:physiology*; D016372:RNA, Antisense; D016337:RNA, Catalytic; D012335:RNA, Ribosomal / Q000235:genetics / Q000502:physiology*; D012261:Ribonucleoproteins / Q000378:metabolism*; D017411:Ribonucleoproteins, Small Nuclear
D016373:DNA, Antisense; D006657:Histones; D012322:RNA Precursors; D016372:RNA, Antisense; D016337:RNA, Catalytic; D012335:RNA, Ribosomal; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear; D012313:RNA; D004247:DNA