Survival and phenol red metabolism in chick embryo hepatocytes cultured in the serum-free medium 199 supplemented with dextran T-500 and antioxidants.
The supplementation of serum-free Parker's medium 199 with Dextran T-500 and antioxidants (taurine or catalase) significantly improved spraeding and survival of chick embryo hepatocytes in primary culture. To decrease cell surface injury, the dispersion of liver tissue by colagenase was replaced by dissociation of liver with EGTA solution. In such chemically defined culture conditions hepatocytes were able to glucoronise phenol red better than they did it when cultured in the presence of serum. These results suggest that Dextran T-500 and factors which prevent peroxidation of membrane lipids can simplify the conditions useful for the culture of quiescent hepatocytes in a serum-free media.
Guzik
K
K
Department of Cell Biology, Jan Zurzycki Institute of Molecular Biology, Jagellonian University, Kraków, Poland.
Reiss
K
K
Korohoda
W
W
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D003911:Dextrans / Q000494:pharmacology*; D008099:Liver / Q000166:cytology* / Q000187:drug effects; D010635:Phenolphthaleins / Q000493:pharmacokinetics*; D010637:Phenolsulfonphthalein / Q000493:pharmacokinetics*
D000975:Antioxidants; D003911:Dextrans; D010635:Phenolphthaleins; D010637:Phenolsulfonphthalein