Lectin-mediated targeting of liposomes to a model surface. An ELISA method.
Wheat germ agglutinin has been conjugated to the surfaces of sonicated phospholipid liposomes by reacting the protein derivatised with N-succinimidyl-S-acetylthioacetate (SATA) with the m-maleimidobenzoyl-N-hydroxysuccinimide (MBS) derivative of dipalmitoylphosphatidylethanolamine (DPPE) incorporated into the liposomal bilayers. The liposomes as characterised by photon correlation spectroscopy had a weight-average radius of 44 +/- 10 nm and the number of WGA molecules per liposome was in the range up to approx. 120. An ELISA method has been developed to assess the efficiency of targeting the conjugated liposomes to the antigenic determinants on a surface coated with glycophorin A (blood group B). For liposomes in which the degree of conjugation was controlled by varying the mol% DPPE-MBS from 3 to 27% targeting efficiency as assessed from the extent of inhibition of the ELISA increased by a factor of 10.
Hutchinson
F J
FJ
Department of Biochemistry & Molecular Biology, University of Manchester, England.
Jones
M N
MN
D015060:1,2-Dipalmitoylphosphatidylcholine; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes / Q000032:analysis; D006021:Glycophorins / Q000032:analysis* / Q000276:immunology; D007202:Indicators and Reagents; D008051:Lipid Bilayers; D008954:Models, Biological; D010714:Phosphatidylethanolamines; D012795:Sialoglycoproteins / Q000032:analysis*; D014909:Wheat Germ Agglutinins
D000939:Epitopes; D006021:Glycophorins; D007202:Indicators and Reagents; D008051:Lipid Bilayers; D010714:Phosphatidylethanolamines; D012795:Sialoglycoproteins; D014909:Wheat Germ Agglutinins; D015060:1,2-Dipalmitoylphosphatidylcholine; C007510:1,2-dipalmitoyl-3-phosphatidylethanolamine