High titre Hog Cholera virus production on Cytodex 3 microcarrier cultures.
In an attempt to produce Hog Cholera virus (HCV) preparations of high titre, optimal growth and trypsinization conditions of PK-15 microcarrier cell cultures were defined. Infecting a PK-15 Cytodex 3 microcarrier culture with HCV increased the yield of virus more than 10 times compared with conventional monolayer culture in Roux flasks.
Caij
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A
National Institute for Veterinary Research, Brussels, Belgium.
De Smet
A
A
Dubois
N
N
Koenen
F
F
D000818:Animals; D002478:Cells, Cultured; D006692:Classical Swine Fever Virus / Q000254:growth & development*; D003911:Dextrans; D014776:Virus Cultivation / Q000379:methods*
D003911:Dextrans; C052839:Cytodex