[Evaluation of a radioimmunoassay of alpha 1-microglobulin (alpha 1-m) with simplified procedures].
A newly established double antibody radioimmunoassay (RIA) was fundamentally and clinically evaluated. Original procedures were partially modified as follows: Sample volume for serum and urine was changed to 25 microliters, and thus 200 mg/l of alpha 1-m standard was prepared using 50 microliters of original standard solution (100 mg/l). The results were satisfactory in sensitivity (0.3 mg/l obtained from -2SD method), intra-assay precision with its coefficient variation (CV) ranging from 3.0 to 7.4%, interassay precision with its CV ranging from 3.0 to 10.7%, and recovery with the mean value of 102.4% in serum and 108.2% in urine respectively. There were no changes about alpha 1-m value between diluted (2 times) and undiluted with high concentration samples. Normal levels of alpha 1-m were less than 25 mg/l in serum and less than 10 mg/l in urine. The present results indicate that the determination of alpha 1-m could be very simple and useful for the most sensitive screening test for the evaluation of renal function.
Matsui
K
K
Department of Nuclear Medicine, Sumitomo Hospital, Osaka, Japan.
Moriuma
H
H
Mishima
C
C
Honda
M
M
Tomonobu
M
M
Kanao
K
K
Fushimi
H
H
D000510:Alpha-Globulins / Q000032:analysis* / Q000652:urine; D005069:Evaluation Studies as Topic; D006801:Humans; D011480:Protease Inhibitors / Q000032:analysis* / Q000097:blood / Q000652:urine; D011863:Radioimmunoassay / Q000379:methods*; D011933:Reagent Kits, Diagnostic
D000510:Alpha-Globulins; D011480:Protease Inhibitors; D011933:Reagent Kits, Diagnostic; C016001:alpha-1-microglobulin