Localization in situ of c-myc mRNA and c-myc protein in adult mouse testis.
It has been suggested that c-myc, one of the proto-oncogenes, plays a role in normal somatic cell proliferation and differentiation. To define whether c-myc is only expressed during somatic cell division or is also expressed during meiotic cell division, the production of c-myc mRNA and protein were investigated in the mouse testis by using in situ hybridization with non-radioactive DNA probes and enzyme immunohistochemistry respectively. For in situ hybridization, T-T dimerized DNA probes were used and DNAs hybridized in situ were detected immunohistochemically using specific antibody against T-T dimer. The results indicate that c-myc mRNA and protein are expressed in a cell-cycle-dependent manner only in spermatogonia and not in spermatocytes and spermatids.
Koji
T
T
Department of Cell Biology, Tokai University School of Medicine, Isehara, Japan.
Izumi
S
S
Tanno
M
M
Moriuchi
T
T
Nakane
P K
PK
D000818:Animals; D007124:Immunoenzyme Techniques; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009693:Nucleic Acid Hybridization; D011518:Proto-Oncogene Proteins / Q000032:analysis* / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis*; D013737:Testis / Q000032:analysis*
D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D012313:RNA