Studies on platelet functions in hirudin plasma.
Comparative studies on platelet responses in citrated, hirudin and heparin plasma were carried out. The adhesion of 111In-labelled rabbit platelets to the subendothelium of rabbit aorta was more pronounced in hirudin plasma than in heparin and citrated plasma. There were no significant differences in the collagen-induced aggregation and secretion of 14C-serotonin of human blood platelets in the three plasma samples. The extent of the ADP-induced aggregation was nearly the same in the three plasma samples, however, the aggregation was reversible in hirudin plasma. Adrenaline induced a small primary aggregation in hirudin plasma whereas in citrated and in heparin plasma the aggregation was a biphasic one. Secretion of 14C-serotonin induced by ADP or adrenaline occurred in citrated plasma only. Hirudin proved to be a suitable anticoagulant for studying platelet functions at physiological calcium concentrations.
Glusa
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E
Institute of Pharmacology and Toxicology, Medical Academy Erfurt, GDR.
Urban
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U
D000244:Adenosine Diphosphate; D001792:Blood Platelets / Q000378:metabolism; D002118:Calcium / Q000097:blood; D002951:Citrates / Q000097:blood; D003094:Collagen; D006493:Heparin / Q000097:blood; D006629:Hirudins / Q000097:blood*; D006801:Humans; D010973:Platelet Adhesiveness / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects; D010979:Platelet Function Tests
D002951:Citrates; D006629:Hirudins; D000244:Adenosine Diphosphate; D006493:Heparin; D003094:Collagen; D002118:Calcium