Effects of suramin, HPA-23 and 3'-azidothymidine triphosphate on the reverse transcriptase of bovine leukaemia virus.
We used purified bovine leukaemia virus (BLV) to test the inhibitory effects of suramin, HPA-23, and 3'-azidothymidine triphosphate (N3dTTP) on the reverse transcriptase activity. The 50% inhibitory concentrations (ID50) of the compounds were determined to be 2.8 mumol/l (suramin), 8.0 mumol/l (HPA-23), and 0.17 mumol/l (N3dTTP). Kinetic analyses of suramin and HPA-23 inhibition are discussed. The observed inhibitory effects emphasize the suitability of BLV as a model virus for investigations of retrovirus chemotherapy.
Reimer
K
K
Institut für Virologie, Humboldt Universität, DDR, Berlin.
Matthes
E
E
Scholz
D
D
Rosenthal
H A
HA
D000818:Animals; D000965:Antimony / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D002417:Cattle; D007700:Kinetics; D001909:Leukemia Virus, Bovine / Q000187:drug effects* / Q000201:enzymology; D008954:Models, Biological; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012190:Retroviridae / Q000187:drug effects*; D018894:Reverse Transcriptase Inhibitors; D013498:Suramin / Q000493:pharmacokinetics / Q000494:pharmacology*; D014414:Tungsten / Q000494:pharmacology*; D017973:Tungsten Compounds; D015215:Zidovudine / Q000494:pharmacology*
D000998:Antiviral Agents; D018894:Reverse Transcriptase Inhibitors; D017973:Tungsten Compounds; D015215:Zidovudine; C009613:ammonium tungsten antimonate hydroxide oxide; D013498:Suramin; D000965:Antimony; D012194:RNA-Directed DNA Polymerase; D014414:Tungsten