[Analysis of corticosteroids in blood plasma using a microcolumn liquid chromatography method].
A microcolumn variant of high-pressure liquid chromatography of human blood plasma corticosteroids using Milikhrom, a Soviet chromatograph, is suggested. The analysis involves hexane treatment of the plasma, chloroform extraction of corticosteroids, concentration of the extract, and chromatography in a 60 X 2 mm column packed with Silasorb sorbent, sph C18 (7.5 micros), methyl alcohol/water mixture (52:48) at a rate of 100 microliter/min and a detection wavelength 240 nm. The method permits an analysis of cortisone, hydrocortisone, corticosterone, and other corticosteroids with concentrations higher than 4 ng/ml.
Baĭkova
L A
LA
Fedorov
V I
VI
Cherkasova
O P
OP
D000305:Adrenal Cortex Hormones / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D006801:Humans
D000305:Adrenal Cortex Hormones
Analiz kortikosteroidov plazmy krovi metodom mikrokolonochnoĭ zhidkostnoĭ khromatografii.