Differential secretion of stimulatory-inhibitory CFU-GM activity by stromal cells from different compartments of mouse bone femur.
We have recently reported the beneficial effect of low oxygen concentration on the growth of fibroblast colony-forming units (CFU-F) isolated from the shaft of the femur (fraction 1), the endosteum (fraction 2), and the compact bone (fraction 3). We now report that the conditioned medium obtained from the CFU-F in the same experiments exhibited stimulatory activity from the compact bone while expressing inhibitory activity from the femoral shaft and endosteum for granulocyte-macrophage CFU (CFU-GM). These data support the presence of a spatial regulatory role for fibroblast-like cells within compartments of mouse bone for CFU-GM.
Gupta
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Department of Internal Medicine, University of Texas Medical Branch, Galveston 77550.
D000818:Animals; D001853:Bone Marrow / Q000502:physiology*; D001854:Bone Marrow Cells; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000502:physiology*; D003470:Culture Media; D005109:Extracellular Matrix / Q000502:physiology*; D005269:Femur; D005347:Fibroblasts / Q000502:physiology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006131:Growth Inhibitors / Q000502:physiology*; D006133:Growth Substances / Q000502:physiology*; D007372:Interferons / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D003115:Colony-Stimulating Factors; D003470:Culture Media; D006131:Growth Inhibitors; D006133:Growth Substances; D014409:Tumor Necrosis Factor-alpha; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D007372:Interferons