A method for simultaneous detection of the transcripts of different cellular genes.
In this paper, a method for preparing radioactive-labelled first-strand cDNA has been described and the major procedures and reaction conditions have been studied. Some advantages provided by this method for simultaneously detecting the expression of multiple cellular genes are discussed.
Liu
Z J
ZJ
Department of Molecular Biology and Biochemistry, Chinese Academy of Medical Sciences, Beijing.
Wu
N H
NH
Shen
Y F
YF
D001345:Autoradiography / Q000379:methods; D002460:Cell Line; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D007700:Kinetics; D008214:Lymphocytes / Q000378:metabolism; D009857:Oncogenes; D010761:Phosphorus Radioisotopes; D011061:Poly A / Q000302:isolation & purification*; D011519:Proto-Oncogenes; D012313:RNA / Q000302:isolation & purification*; D012333:RNA, Messenger; D014158:Transcription, Genetic
D015342:DNA Probes; D004273:DNA, Neoplasm; D010761:Phosphorus Radioisotopes; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA