Gene overlap results in a viral protein having an RNA binding domain and a major coat protein domain.
L-A double-stranded RNA (dsRNA) replicates in vivo in yeast in a conservative, asynchronous (first [+] strand then [-] strand), intraviral process. New particles are formed by packaging (+) strands. Added viral (+) single-stranded RNA (ssRNA) is specifically bound by empty virus-like particles (VLPs) and, in a reaction requiring a host factor, is converted in vitro to dsRNA. We find that the isolated binding complex replicates only if it was formed in the presence of the host factor. The VLP minor 180 kd protein, but not the major coat protein, has ssRNA binding activity on Western blots. The 180 kd protein shares a common antigenic domain with the major coat protein, the latter known to be encoded by L-A dsRNA. The 180 kd protein, but not the major coat protein, also shares an antigenic domain with a sequence encoded by the 3' end of the L-A (+) strand. Thus the 180 kd protein is also encoded by L-A dsRNA and consists of a major coat protein domain and a ssRNA binding domain.
Fujimura
T
T
Section on Genetics of Simple Eukaryotes, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892.
Wickner
R B
RB
D000595:Amino Acid Sequence; D001665:Binding Sites; D002213:Capsid / Q000235:genetics*; D002352:Carrier Proteins / Q000378:metabolism; D034982:Integration Host Factors; D008958:Models, Molecular; D008970:Molecular Weight; D012313:RNA / Q000378:metabolism*; D012330:RNA, Double-Stranded / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014764:Viral Proteins / Q000378:metabolism*
D002352:Carrier Proteins; D034982:Integration Host Factors; D012330:RNA, Double-Stranded; D014764:Viral Proteins; D012313:RNA