title: Effect of confluence on endocytosis by 3T3 fibroblasts: increased rate of pinocytosis and accumulation of residual bodies.
abstract: We have compared lysosomal enzyme distributions on density gradients and rates of transport of endocytic markers for actively-growing and confluent cells. While it has been previously established that mammalian cells accumulate lysosomal enzymes during quiescence, we show that this accumulation is predominantly in residual bodies (p greater than 1.12 g/ml) rather than in dense lysosomes (p = 1.08-1.10 g/ml) and does not represent a change in the endosomal and lysosomal enzyme content. The accumulation is not caused by a change in the rate of production of dense lysosomes, since the rate of transfer of epidermal growth factor (EGF) from light to dense compartments is the same between confluent and subconfluent cells. Confluent cultures have a higher rate of initial pinocytosis, and a higher rate of retroendocytosis and/or recycling, causing a net lower rate of accumulation of fluid-phase material. The accumulation of residual bodies in confluent cultures may be caused by a lower rate of exocytosis of their contents and/or a lack of dilution by cell division. The data indicate that the impact of culture confluence must be carefully assessed in experiments designed to analyze endocytic pathways.
authors: Department of Biological Sciences, Carnegie-Mellon University, Pittsburgh, PA 15213.
authors: M
authors: M
authors: Roederer
authors: R W
authors: RW
authors: Mays
authors: R F
authors: RF
authors: Murphy
mesh_terms: D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002478:Cells, Cultured; D003911:Dextrans / Q000378:metabolism; D004705:Endocytosis; D004815:Epidermal Growth Factor / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D005470:Fluorometry; D006867:Hydrolases / Q000378:metabolism; D002479:Inclusion Bodies / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D008247:Lysosomes / Q000201:enzymology / Q000378:metabolism; D051379:Mice; D008854:Microscopy, Electron; D010873:Pinocytosis
chemical_list: D003911:Dextrans; D004815:Epidermal Growth Factor; D006867:Hydrolases