title: Study on the mechanism of interferon action (XI)--Effect of pppA2'p5'A on the level of cAMP and cGMP in macrophages.
abstract: In this paper, the increase of cellular cAMP and cGMP levels in macrophages induced by ppA2'p5' A2'p5'A (briefly 2'-5'P3A3) is first reported. The optimal concentration of 2'-5' P3A3 for the elevation of cellular cGMP to the highest level is 10(-7)-10(-6) mol/L, while that for cAMP is 10(-7) mol/L. The time for cGMP to reach its peak value is 15 min and that for cAMP is 2 h, when the cells are treated with 2'-5' P3A3 at 10(-7) mol/L, which is the optimal concentration for developing biological effect of macrophages (phagocytosis). These results suggest that cGMP and cAMP may be related to, or may be the mediators for, 2'-5'P3A3 action.
authors: Shanghai Institute of Biochemistry, Academia Sinica.
authors: G S
authors: GS
authors: Yin
authors: X Q
authors: XQ
authors: Du
authors: G X
authors: GX
authors: Wang
authors: X M
authors: XM
authors: Wang
authors: M X
authors: MX
authors: Zhang
authors: W Z
authors: WZ
authors: Zhao
authors: Y
authors: Y
authors: Chen
authors: M H
authors: MH
authors: Yao
authors: H D
authors: HD
authors: Zheng
authors: X Y
authors: XY
authors: Liu
mesh_terms: D000227:Adenine Nucleotides / Q000494:pharmacology*; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism*; D007372:Interferons / Q000502:physiology*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009843:Oligoribonucleotides / Q000494:pharmacology*; D051381:Rats
chemical_list: D000227:Adenine Nucleotides; D009843:Oligoribonucleotides; C016964:adenosine triphosphate adenosine monophosphate adenosine monophosphate; D007372:Interferons; D000242:Cyclic AMP; D006152:Cyclic GMP