Tumor promoters accentuate phosphorylation of PO: evidence for the presence of protein kinase C in purified PNS myelin.
The effects of carbon tetrachloride, methylene chloride and chloroform on phosphorylation of PO was examined. The results of the dose response curve revealed that carbon tetrachloride (0.67%), methylene chloride (2%) and chloroform (1%) induced phosphorylation of PO by approximately 4, 6, and 12-fold, respectively. PO was found to be phosphorylated on the serine residue, and the phosphorylation of the serine residue was markedly increased when PO was phosphorylated in the presence of these compounds. Since tumor promoters, carbon tetrachloride and chloroform, have been shown to activate protein kinase C in platelets it is postulated that the increased phosphorylation of PO may result from the activation of myelin associated protein kinase C. The presence of phospholipid sensitive Ca2+-dependent protein kinase (protein kinase C) in in purified nerve myelin was demonstrated by increased phosphorylation of PO in the presence of Ca2+ and phosphatidylserine.
Agrawal
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Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri 63110.
Agrawal
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D000818:Animals; D002251:Carbon Tetrachloride / Q000494:pharmacology; D002273:Carcinogens / Q000494:pharmacology*; D002725:Chloroform / Q000494:pharmacology*; D006843:Hydrocarbons, Chlorinated / Q000494:pharmacology*; D008752:Methylene Chloride / Q000494:pharmacology*; D018993:Myelin P0 Protein; D009185:Myelin Proteins / Q000378:metabolism*; D009186:Myelin Sheath / Q000187:drug effects / Q000201:enzymology*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D002273:Carcinogens; D006843:Hydrocarbons, Chlorinated; D018993:Myelin P0 Protein; D009185:Myelin Proteins; D008752:Methylene Chloride; D002725:Chloroform; D002251:Carbon Tetrachloride; D011494:Protein Kinases