Action of iloprost and PGE1 on global ischemic and reperfused myocardium: a 31P-NMR-study.
The influence of iloprost, PGE1 and of the combined application of iloprost and PGE1 on high energy phosphate contents was investigated in isolated rat hearts perfused aerob at 37 degrees C in Langendorff mode. Changes in creatine phosphate, ATP and inorganic phosphate were registered during 20 minutes of global ischemia and 56 minutes of reperfusion with 31P-NMR-spectroscopic methods starting drug application prior to ischemia simultaneously with onset of heart perfusion. Most effective in preservation of high energy phosphates was the combined application of PGE1 and iloprost resulting in a creatine phosphate/inorganic phosphate ratio of 103.2 +/- 30.9% of pre-ischemia values compared to 52.5 +/- 6.1% in control group without drug application 0-5 minutes after onset of reperfusion, 148.8 +/- 24.8% vs 78.8 +/- 15.2% at 6-11 minutes of reperfusion and 116.6 +/- 16% vs 68.9 +/- 12.7% at 12-17 minutes of reperfusion. The same trend was observed employing ATP/inorganic phosphate ratio. The improved energy state in reperfused hearts following application of PGE1 and iloprost in combination is presumed to be supported by a reduction of the loss of high energy phosphates (HEP) during global ischemia and by a cytoprotective effect of iloprost immediately after starting reperfusion.
Pissarek
M
M
Dept. Cardiovasc. Surg., Karl-Marx-Univ., Leipzig, GDR.
Gründer
W
W
Keller
T
T
Goos
H
H
Mest
H J
HJ
Krause
E G
EG
D000527:Alprostadil / Q000494:pharmacology*; D000818:Animals; D002317:Cardiovascular Agents / Q000494:pharmacology*; D003327:Coronary Disease / Q000503:physiopathology*; D004347:Drug Interactions; D004734:Energy Metabolism / Q000187:drug effects; D011464:Epoprostenol / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects*; D016285:Iloprost; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D010477:Perfusion; D010758:Phosphorus; D051381:Rats; D011919:Rats, Inbred Strains
D002317:Cardiovascular Agents; D010758:Phosphorus; D011464:Epoprostenol; D000527:Alprostadil; D016285:Iloprost