Alzheimer's disease: a new evidence for common epitopes between microtubule associated protein Tau and paired helical filaments (PHF): demonstration at the electron microscope level by a double immunogold labelling.
Paired helical filaments (PHF) are neuronal landmarks of Alzheimer's disease. These pathological filaments are antigenically related to proteins present in the normal cytoskeleton, particularly to microtubule associated protein Tau. The evidence for these common epitopes was studied on sections of cortex from Alzheimer brains after Araldite embedding. Two rabbit immune sera were used: one was raised against PHF isolated from Alzheimer cortex; the other against Tau proteins extracted from bovine cortex. The comparison of adjacent semi-thin sections alternatively treated with anti-PHF and anti-Tau immune sera reveals that both stained degenerating neurofibrils in pyramidal perikarya and in neurites surrounding senile plaques. On ultra-thin sections, double immunogold labelling of PHF was obtained. These results are in accordance with the hypothesis that Tau proteins are major antigenic components of PHF.
Defossez
A
A
Unité INSERM 156, Faculté de Médecine, Lille, France.
Beauvillain
J C
JC
Delacourte
A
A
Mazzuca
M
M
D000544:Alzheimer Disease / Q000276:immunology / Q000473:pathology*; D002540:Cerebral Cortex / Q000276:immunology / Q000648:ultrastructure; D000939:Epitopes / Q000032:analysis*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D008869:Microtubule-Associated Proteins / Q000276:immunology*; D009419:Nerve Tissue Proteins / Q000276:immunology*; D009454:Neurofibrils / Q000276:immunology* / Q000648:ultrastructure; D016875:tau Proteins
D000939:Epitopes; D007106:Immune Sera; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D016875:tau Proteins