A 70 kDa microtubule-associated protein in NIL8 cells comigrates with the 70 kDa heat shock protein.
When eukaryotic cells are exposed to environmental stress such as elevated temperature, the synthesis of heat shock proteins (HSP) is stimulated. We have raised a monoclonal antibody to a 70 kDa cytoskeleton-associated protein; this antibody also appears to recognize HSPs 68, 70 and 90, as well as an additional 40 kDa non-heat shock protein. We have used this monoclonal antibody to study the localization of the 70 kDa protein in the cytoskeletons of NIL8 hamster fibroblasts. By selective sequential solubilization of the components of NIL8 cells and analysis of the resulting cytoskeletal preparations by Western blot technique and indirect immunofluorescence, we have shown that the 70 kDa protein is associated with microtubules in mitotic and interphase cells and comigrates with HSP70 on 2-dimensional gel electrophoretigrams.
Weller
N K
NK
Center for Cancer Research, Massachusets Institute of Technology, Cambridge 02139.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015153:Blotting, Western; D002460:Cell Line; D006224:Cricetinae; D003429:Cross Reactions; D003599:Cytoskeleton / Q000032:analysis; D004330:Drosophila; D015180:Electrophoresis, Gel, Two-Dimensional; D000939:Epitopes / Q000276:immunology; D005347:Fibroblasts / Q000032:analysis / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006360:Heat-Shock Proteins / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007399:Interphase; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000032:analysis* / Q000378:metabolism; D008870:Microtubules / Q000032:analysis / Q000187:drug effects / Q000378:metabolism; D008938:Mitosis; D051381:Rats; D012441:Saccharomyces cerevisiae
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006360:Heat-Shock Proteins; D008869:Microtubule-Associated Proteins