Induction of intestinal microsomal enzymes by polycyclic aromatic hydrocarbons.
1. The efficacy of induction of intestinal microsomal aryl hydrocarbon hydroxylase (AHH), 7-ethoxyresorufin o-deethylase and cytochrome P-450 by various polycyclic aromatic hydrocarbons were studied. 2. The greatest induction of the specific MFO activities tested was produced by 2,3,7,8-tetra-chlorodibenzo-p-dioxin (TCDD) with 3-methyl-cholanthrene (MC) a close second. 3. Rank order of efficacy of induction of spectrally determined cytochrome P-450 was 2,3,7,8-tetra-chlorodibenzo-p-dioxin (TCDD), Arochlor 1254 (ARO), 3-methylcholanthrene (MC) and benz(a)anthracene(BA). 4. No unique protein peaks were found when the sodium dodecyl sulfate-polyacrylamide gel electrophoresis of MC treated intestinal microsomes were prepared.
Hassing
J M
JM
College of Pharmacy, University of Nebraska, Medical Center, Omaha 68105.
al-Turk
W A
WA
Stohs
S J
SJ
D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis; D015786:Cytochromes b5 / Q000096:biosynthesis; D004247:DNA / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction / Q000187:drug effects; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000187:drug effects / Q000201:enzymology; D007421:Intestine, Small / Q000187:drug effects / Q000201:enzymology; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000096:biosynthesis*; D010078:Oxazines / Q000378:metabolism; D011083:Polycyclic Compounds / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D010078:Oxazines; D011083:Polycyclic Compounds; D011506:Proteins; D001564:Benzo(a)pyrene; C007649:ethoxyresorufin; D012313:RNA; D004247:DNA; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases