The stimuli releasing histamine from murine bone marrow-derived mast cells. 1. The presence of P2-purinoceptors.
We have examined the histamine-releasing effects of 4 different stimuli on murine bone marrow-derived mast cells (BMMC). We obtained 10(9) BMMC from one femur of a CBA/J mouse when it was cultured in the presence of conditioned medium from WEHI-3 cells for 4 weeks. When these cells were sensitized with monoclonal anti-DNP IgE antibodies, DNP35HSA antigen, ionomycin, thrombin and ATP induced 70%, 70%, 40% and 60% histamine release from BMMC, respectively. Thrombin induced rapid degranulation, whereas the kinetics of histamine release by other stimuli reached a plateau after 5 min. ADP, AMP and GTP as well as ATP but not adenosine caused histamine release from BMMC, suggesting the presence of P2-purinoceptors on these cells.
Nakamura
H
H
Saito
H
H
Ikura
Y
Y
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D004140:Dinitrophenols / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects*; D015759:Ionomycin / Q000494:pharmacology*; D007700:Kinetics; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008808:Mice, Inbred CBA; D011983:Receptors, Purinergic / Q000378:metabolism*; D012709:Serum Albumin / Q000494:pharmacology*; D013917:Thrombin / Q000494:pharmacology*
D004140:Dinitrophenols; D011983:Receptors, Purinergic; D012709:Serum Albumin; C058064:dinitrophenyl-human serum albumin conjugate; D015759:Ionomycin; D000255:Adenosine Triphosphate; D013917:Thrombin