A photoreversible phytochrome affinity column chromatography for putative phytochrome receptor studies.
Using a photoreversible phytochrome affinity column, we have isolated a small RNA molecule that binds to the affinity column. The RNA was identified by a A260/A280 ratio of 2.0, hypochromicity, orcinol, ribonuclease A and DNase tests. Agarose gel electrophoresis and circular dichroic spectral characteristics also indicate it to be a small RNA molecule. The successful preparation of a photoreversible phytochrome affinity column, the possible usage of the column and the significance of the RNA binding have been discussed.
Singh
B R
BR
Department of Chemistry and Biochemistry, Texas Tech University, Lubbock 79409.
Chai
Y G
YG
Robertson
D T
DT
Song
P S
PS
D002846:Chromatography, Affinity / Q000379:methods; D003600:Cytosol / Q000032:analysis; D002523:Edible Grain; D010834:Phytochrome / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D010944:Plants / Q000032:analysis; D012313:RNA / Q000302:isolation & purification* / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000302:isolation & purification; D012685:Sepharose / Q000031:analogs & derivatives; D013053:Spectrophotometry
D010940:Plant Proteins; D011956:Receptors, Cell Surface; D010834:Phytochrome; D012313:RNA; C055194:Affi-Gel 15; D012685:Sepharose