Genetic and biochemical analysis of Shigella dysenteriae 1 O antigen polysaccharide biosynthesis in Escherichia coli K-12: 9 kb plasmid of S. dysenteriae 1 determines addition of a galactose residue to the lipopolysaccharide core.
Production of the somatic antigen, O-specific polysaccharide of Shigella dysenteriae 1 is determined by the chromosomal rfb gene cluster and the rfp gene located on the 9 kb plasmid pHW400 carried by this organism. When transferred to Escherichia coli K-12, which produces lipopolysaccharide consisting only of core oligosaccharide linked to lipid A, rfp gene-containing plasmids caused modification of the core oligosaccharide leading to the appearance of core molecules with new electrophoretic mobilities. Chemical analysis of the modified core has shown that it is substituted with a galactose residue which is the first sugar of the O-polysaccharide repeat unit.
Sturm
S
S
Department of Medical Biochemistry, University of Geneva, Switzerland.
Jann
B
B
Jann
K
K
Fortnagel
P
P
Timmis
K N
KN
D000942:Antigens, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005690:Galactose / Q000235:genetics; D008070:Lipopolysaccharides / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D019081:O Antigens; D010957:Plasmids; D012762:Shigella dysenteriae / Q000235:genetics* / Q000276:immunology / Q000378:metabolism
D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D019081:O Antigens; D005690:Galactose