title: Specificity of riboprobes for intracellular RNA in hybridization histochemistry.
abstract: The specificity of the probe for intracellular RNA during hybridization histochemistry is usually confirmed by loss of the signal following ribonuclease pre-treatment of the target. Interpretation of such controls is complicated when single-stranded (asymmetric) RNA probes (riboprobes) are used for in situ hybridization, since the probes themselves may be degraded by residual ribonuclease. A protocol using the Ca2+-dependent enzyme, micrococcal nuclease, is presented to circumvent these difficulties.
authors: Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
authors: D J
authors: DJ
authors: Williamson
mesh_terms: D000818:Animals; D003115:Colony-Stimulating Factors / Q000235:genetics; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000235:genetics; D006651:Histocytochemistry / Q000379:methods*; D007377:Interleukin-3 / Q000235:genetics; D051379:Mice; D008836:Micrococcal Nuclease / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*
chemical_list: D003115:Colony-Stimulating Factors; D006133:Growth Substances; D007377:Interleukin-3; D012333:RNA, Messenger; D012313:RNA; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D008836:Micrococcal Nuclease