Oxygen free radical-induced histamine release during intestinal ischemia and reperfusion.
Acute mesenteric ischemia is highly lethal and therefore a serious problem for surgery and intensive care medicine; accordingly its pathophysiology warrants further study. Oxygen free radicals (OFR) play a role in the intestinal mucosal damage that develops during reperfusion after ischemia. Histamine (H) is generally released in various types of tissue ischemia. The link between H release and OFR has only been studied in in vitro systems. We tested the hypothesis that OFR may be involved in H release following reperfusion of the ischemic gut. The artery supplying a segment of the ileum was occluded for 1 or 2 h in anesthetized dogs. On reperfusion, a release of H into the venous effluent of the segment was demonstrated. Pretreatment of the animals with allopurinol (an inhibitor of xanthine oxidase), or with MTDQ-DA [6,6'-methylene-bis(2,2-dimethyl-4-methanesulfonic acid sodium-1,2-dihydroquinoline)], a superoxide anion scavenger, or with a combination of allopurinol and MTDQ-DA resulted in an inhibition of H release. We conclude that OFR may play a role in the local H release following intestinal ischemia.
Boros
M
M
Institute of Experimental Surgery, Szent-Györgyi Albert Medical University, Szeged, Hungary.
Kaszaki
J
J
Nagy
S
S
D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D004285:Dogs; D005260:Female; D005609:Free Radicals; D006632:Histamine / Q000097:blood; D006636:Histamine Release / Q000187:drug effects*; D007082:Ileum / Q000098:blood supply*; D007511:Ischemia / Q000378:metabolism*; D008297:Male; D008641:Mesenteric Vascular Occlusion / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D011804:Quinolines / Q000494:pharmacology; D015427:Reperfusion Injury / Q000378:metabolism*
D000975:Antioxidants; D005609:Free Radicals; D011804:Quinolines; D000493:Allopurinol; D006632:Histamine; C044964:6,6'-methylenebis(2,2-dimethyl-4-methanesulfonic acid-1,2-dihydroquinoline); D010100:Oxygen