Myelin basic protein binds GTP at a single site in the N-terminus.
Myelin basic protein from normal human brain was ADP-ribosylated with Cholera toxin, but not with Pertussis toxin. It bound azido-GTP at a single site in the N-terminal tetrapeptide at the Gln residue. The binding was considerably reduced when GppNHp was present during azido-GTP binding and totally inhibited when GTP gamma S was present. The relevance of this specific binding is not understood at this time.
Chan
C K
CK
Hospital for Sick Children, Department of Biochemistry, Toronto, Ontario, Canada.
Ramwani
J
J
Moscarello
M A
MA
Chan
K C
KC
Ranwani
J
J
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis; D001386:Azides / Q000378:metabolism; D001665:Binding Sites; D002772:Cholera Toxin / Q000494:pharmacology; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D006801:Humans; D004676:Myelin Basic Protein / Q000378:metabolism*; D037342:Pertussis Toxin; D013873:Thionucleotides / Q000494:pharmacology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
C021961:8-azidoguanosine triphosphate; D000596:Amino Acids; D001386:Azides; D004676:Myelin Basic Protein; D013873:Thionucleotides; D010566:Virulence Factors, Bordetella; D000246:Adenosine Diphosphate Ribose; D006165:Guanylyl Imidodiphosphate; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate; D002772:Cholera Toxin; D037342:Pertussis Toxin