[Conformation limited nucleoside-5'-phosphates as termination substrates for DNA-polymerases].
We have investigated the ability of some nucleoside 5'-triphosphate analogues to terminate the DNA synthesis catalyzed by calf thymus DNA polymerase alpha and terminal deoxynucleotidyl transferase, rat liver DNA polymerase beta, E. coli DNA polymerase I (Klenow's fragment) and AMV reverse transcriptase. It has been shown that lyxoanhydronucleoside 5'-triphosphates terminate DNA synthesis catalyzed by reverse transcriptase and terminal deoxynucleotydil transferase. 2',3'-O-Isopropylidenecytidine 5'-triphosphate inhibits the DNA synthesis catalyzed by reverse transcriptase and DNA polymerase beta and its moiety was incorporated in the place of dTMP residue. Riboanhydroadenosine 5'-triphosphate reveals the properties of an effective termination substrate for all the DNA polymerases studied. This is the first attempt to investigate nucleotide analogues with the restricted conformation of the carbohydrate moiety as termination substrates for several prokaryotic and eukaryotic DNA polymerases.
Chidzhavadze
Z G
ZG
Bibilashvili
R Sh
RSh
Rozovskaia
T A
TA
Atrazhev
A M
AM
Tarusova
N B
NB
Minasian
Sh Kh
ShKh
Diatkina
N B
NB
Atrazheva
E D
ED
Kukhanova
M K
MK
Papchikhin
A V
AV
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000096:biosynthesis*; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism; D004256:DNA Polymerase I / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009711:Nucleotides / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013379:Substrate Specificity
D009711:Nucleotides; D004247:DNA; D004253:DNA Nucleotidylexotransferase; D012194:RNA-Directed DNA Polymerase; D004256:DNA Polymerase I; D004259:DNA-Directed DNA Polymerase
Konformatsionno ogranichennye nukleozid-5'-trifosfaty kak terminatornye substraty DNK-polimeraz.