Structural studies of the O-antigenic polysaccharide of Escherichia coli O86, which possesses blood-group B activity.
The O-specific side-chains of the lipopolysaccharide from Escherichia coli O86:K2:H2 have been investigated using n.m.r. spectroscopy, methylation analysis, and specific degradations, and shown to be composed of the pentasaccharide repeating-unit (formula; see text) which represents the biological repeating-unit. The blood-group B activity was confirmed by an enzyme-linked immunosorbent assay.
Andersson
M
M
Department of Vaccine Development and Production, National Bacteriological Laboratory, Stockholm, Sweden.
Carlin
N
N
Leontein
K
K
Lindquist
U
U
Slettengren
K
K
D000017:ABO Blood-Group System / Q000276:immunology*; D000942:Antigens, Bacterial / Q000032:analysis*; D002240:Carbohydrate Sequence; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D004926:Escherichia coli / Q000032:analysis* / Q000276:immunology; D006801:Humans; D008070:Lipopolysaccharides / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data
D000017:ABO Blood-Group System; D000942:Antigens, Bacterial; D000939:Epitopes; D008070:Lipopolysaccharides