Epitope mapping of the monoclonal antibody FAC2 on the apoprotein of Cpa-1 in photosystem II.
Using a combination of cyanogen bromide cleavage and endoproteinase digestion we have shown that the putative epitope for the monoclonal antibody FAC2 lies in the region 360Pro(-391)Ser on the apoprotein of CPa-1. This region lies entirely within the large extrinsic loop of this protein. We have shown previously that the epitope of FAC2 becomes exposed in oxygen-evolving membranes upon treatment with alkaline Tris which releases all four of the manganese associated with the oxygen-evolving site of photosystem II. The epitope is not exposed, however, after CaCl(2) treatment and exposure to low concentrations of chloride, conditions which lead to the release of two of the four manganeses associated with the oxygen-evolving site. These results suggest that, upon release of the chloride-insensitive manganese from photosystem II membranes, a conformational change occurs which leads to the exposure of 360Pro(-391)Ser on CPa-1 to the monoclonal antibody FAC2.
Frankel
L K
LK
Department of Botany, Louisiana State University, USA.
Bricker
T M
TM
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001059:Apoproteins / Q000276:immunology; D001665:Binding Sites; D015153:Blotting, Western; D002734:Chlorophyll / Q000276:immunology*; D000939:Epitopes; D045342:Light-Harvesting Protein Complexes; D008565:Membrane Proteins / Q000276:immunology*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins / Q000276:immunology*; D011487:Protein Conformation; D012995:Solubility
D000911:Antibodies, Monoclonal; D001059:Apoproteins; D000939:Epitopes; D045342:Light-Harvesting Protein Complexes; D008565:Membrane Proteins; D010446:Peptide Fragments; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010940:Plant Proteins; D002734:Chlorophyll