[Isolation and characterization of a new class of cytoplasmic RNP-particles from rabbit reticulocytes containing 7SL RNA].
Ribonucleoprotein particles with sedimentation coefficient of 12-14S were isolated from ribosome-free extracts of rabbit reticulocytes. The particles contain one RNA molecule, whose relative electrophoretic mobility and the 3'-terminal nucleotide sequence correspond to those of the 7SL RNA from mammalian cells and one type of polypeptide chains with a molecular weight of 80,000-85,000 Da. The nucleic component of these particles is identical to that of SRP from dog pancreatic cells but differs from the latter by the protein component.
Avanesov
A Ts
ATs
D000818:Animals; D001483:Base Sequence; D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D012313:RNA / Q000302:isolation & purification*; D011817:Rabbits; D012156:Reticulocytes / Q000032:analysis*; D012261:Ribonucleoproteins / Q000032:analysis / Q000302:isolation & purification*
D012261:Ribonucleoproteins; D012313:RNA
Vydelenie i kharakteristika novogo klassa tsitoplazmaticheskikh RNP-chastits iz retikulotsitov krolika, soderzhashchikh 7SL RNK.