title: Cohesive properties of terminally differentiated keratinocytes.
abstract: The stratum corneum can be dissociated into single squames by several extraction methods: mechanically, with organic solvents, and with detergents. We have performed studied of reaggregation from single squames prepared by these methods. Mechanically dispersed corneocytes recombined into a lamellar-like structure closely resembling intact stratum corneum. Squames obtained by dissociation in ether formed a multilayered structure which differed from the intact tissue in the collapse of the bilayered lamellar structure into a single lucent band with osmiophilic deposits. Squames obtained after extraction with detergent failed to reaggregate.
authors: Department of Dermatology, University of Texas Medical Branch, Galveston.
authors: M M
authors: MM
authors: Brysk
authors: S
authors: S
authors: Rajaraman
authors: P
authors: P
authors: Penn
authors: E
authors: E
authors: Barlow
authors: T
authors: T
authors: Bell
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D002469:Cell Separation / Q000379:methods; D003902:Detergents / Q000494:pharmacology; D000078404:Epidermal Cells; D004817:Epidermis / Q000032:analysis / Q000187:drug effects; D006652:Histological Techniques; D006801:Humans; D007633:Keratins / Q000032:analysis*; D012997:Solvents / Q000494:pharmacology; D013314:Stress, Mechanical
chemical_list: D003902:Detergents; D012997:Solvents; D007633:Keratins