In-vitro bioassays for cholecystokinin.
Two in-vitro methods for the bioassay of cholecystokinin (CCK) are described. They should be useful for determinations of potencies of pharmaceutical formulations of CCK. Both are based on the hormone's ability to stimulate exocrine pancreas. The stimulatory effect on 45Ca efflux from preloaded suspended acinar cells or on release of amylase from pancreatic acini from the guinea-pig has been recorded. The assay based on stimulation of 45Ca outflux is more specific and precise than the method using measurements of amylase secretion. Therefore, the calcium assay was further validated against the conventional guinea-pig gall-bladder contraction assay. Both methods gave similar potency readings but the results from the in-vitro assay were more precise.
Sjödin
L
L
Department of Drugs, National Board of Health and Welfare, Uppsala, Sweden.
Nederman
T
T
Olsson
P A
PA
Viitanen
E
E
D000681:Amylases / Q000378:metabolism; D000818:Animals; D001681:Biological Assay; D002118:Calcium / Q000378:metabolism; D002132:Calcium Radioisotopes; D002766:Cholecystokinin / Q000032:analysis* / Q000494:pharmacology; D005704:Gallbladder / Q000187:drug effects; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D010179:Pancreas / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D012844:Sincalide / Q000494:pharmacology
D002132:Calcium Radioisotopes; D002766:Cholecystokinin; D000681:Amylases; D012844:Sincalide; D002118:Calcium