A new method of the immunohistochemical detection of cellular antigens for light and electron microscopy.
A new immunohistochemical method for light and electron microscopy of tissue- and cell-specific antigens by using ferric colloid-labeled antibody is presented. The antibodies labeled with the cationic cacodylate ferric colloid are stable and bind specifically to the target antigens to show clearly the site of antigens in tissue sections and on free cells by Prussian blue reaction for light microscopy and by the specific figure of electron opaque ferric colloid particles for electron microscopy. The staining procedure is very simple and it gives clear picture. So the method will be of beneficial for general laboratory use in immunohistochemical researches.
Seno
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S
Division of Pathology, Shigei Medical Research Institute, Okayama, Japan.
Akita
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M
Hsueh
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CL
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D002101:Cacodylic Acid; D005290:Ferric Compounds; D006801:Humans; D007074:Immunoglobulin G; D007150:Immunohistochemistry / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D008854:Microscopy, Electron / Q000379:methods*; D013194:Staining and Labeling; D013552:Swine
D000954:Antigens, Surface; D005290:Ferric Compounds; D007074:Immunoglobulin G; D002101:Cacodylic Acid