Neurons in the medial cortex give rise to Timm-positive boutons in the cerebral cortex of lizards.
The origin of Timm-positive presynaptic boutons in the cerebral cortex of the lizard, Podarcis hispanica, was investigated by injections of horseradish peroxidase (HRP)-saponine in Timm-positive areas, i.e. the dorsal and dorsomedial cortices. A broad retrograde labelling of cell somata in the medial cortex was found. Injections of HRP-saponine in the medial cortex resulted in broad anterograde labelling of boutons located in the Timm-positive zones. A double-labelling of the HRP labelled boutons was obtained by using the Neo-Timm or the sulphide-osmium methods. The present results suggest that neurons of the medial cortex send axons that terminate in Timm-positive boutons in the cerebral cortex of lizards.
Lopez-Garcia
C
C
Catedra de Citologia e Histologia, Facultad de Ciencias Biologicas, Universidad de Valencia, Spain.
Martinez-Guijarro
F J
FJ
D000818:Animals; D002540:Cerebral Cortex / Q000032:analysis* / Q000648:ultrastructure; D006651:Histocytochemistry; D006735:Horseradish Peroxidase; D008116:Lizards / Q000033:anatomy & histology / Q000378:metabolism*; D008854:Microscopy, Electron; D009411:Nerve Endings / Q000032:analysis* / Q000648:ultrastructure; D013194:Staining and Labeling; D013572:Synaptic Vesicles / Q000032:analysis / Q000648:ultrastructure; D015032:Zinc / Q000032:analysis*
D006735:Horseradish Peroxidase; D015032:Zinc