title: Synovial microenvironment-T cell interactions. Human T cells bind to fibroblast-like synovial cells in vitro.
abstract: Synovitis in rheumatoid arthritis is characterized by infiltration of the synovium by T and B lymphocytes and monocytes, as well as by the proliferation of synovial lining cells, fibroblasts, and endothelial cells. To study synovial cell-T cell interactions in vitro, we established cultures of fibroblast-like synovial cells, and used these cells in a synovial cell-T cell binding assay. Using T cells at various stages of differentiation and activation, we found that human thymocytes and mitogen-activated peripheral blood T cells bound to fibroblast-like synovial cells, whereas fresh peripheral blood T cells did not. Moreover, activated T cells from inflammatory synovial tissue or from synovial fluid also bound to fibroblast-like synovial cells cultured in vivo. Antibodies against certain epitopes of the T cell CD2 (35.1) and synovial cell lymphocyte function-associated antigen-3 (LFA-3) (TS2/9) molecules inhibited synovial cell-thymocyte binding. However, these same anti-CD2 and anti-LFA-3 antibodies only partially inhibited synovial cell binding to activated normal peripheral blood T cells. Moreover, T cells from inflammatory synovium from rheumatoid arthritis and psoriatic arthritis patients also bound to synovial cells in vitro. These findings demonstrate that fibroblast-like synovial cells are capable of binding to human T cells in vitro, and suggest that during the course of inflammatory synovitis, synovial fibroblast-T cell interactions may occur in vivo.
authors: Department of Medicine, Duke University Medical Center, Durham, NC 27710.
authors: B F
authors: BF
authors: Haynes
authors: B J
authors: BJ
authors: Grover
authors: L P
authors: LP
authors: Whichard
authors: L P
authors: LP
authors: Hale
authors: J A
authors: JA
authors: Nunley
authors: D E
authors: DE
authors: McCollum
authors: K H
authors: KH
authors: Singer
mesh_terms: D000943:Antigens, Differentiation / Q000276:immunology; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D018801:CD2 Antigens; D002478:Cells, Cultured; D000939:Epitopes / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000276:immunology; D010003:Osteoarthritis / Q000276:immunology*; D011971:Receptors, Immunologic / Q000276:immunology; D013583:Synovial Membrane / Q000166:cytology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
chemical_list: D000943:Antigens, Differentiation; D018801:CD2 Antigens; D000939:Epitopes; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic