Suppressor factor secreted by T hybridoma established from peripheral blood lymphocytes of a bone marrow transplantation patient. I. Establishment of human T-cell hybridoma and partial characterization of suppressor factor.
A stable human T-cell hybridoma was established by cell fusion between activated human peripheral blood lymphocytes from an allogeneic bone marrow transplantation patient and the JD1-17 cell line, a subclone of the human T leukemia Jurkat cell line. This hybrid clone 1-8, which bore the surface phenotype of suppressor cells (CD8+HNK1+), spontaneously secreted a factor which, at high dilutions, suppressed the responses of T and B cells induced by mitogens and alloantigens. This suppressor factor was found to be heat-resistant (56 degrees C, 30 min), stable at alkaline but not acid pH, unaffected by 2-mercaptoethanol, and sensitive to trypsin. Preparative isoelectric focusing revealed an isoelectric point of 5.35. The suppressor activity was selectively absorbed by blast T cells. By gel filtration on Sephacryl S-200 and HPLC, the suppressor activity was found in two peaks corresponding to 40-45 kDa (monomer) and 90-95 kDa (dimer).
Matsuo
T
T
Laboratoire d'Immunologie Cellulaire IRSC, CNRS, Villejuif, France.
Watanabe
S
S
Bouvet
J P
JP
Kolb
J P
JP
Quan
C P
CP
D000042:Absorption; D001685:Biological Factors / Q000378:metabolism / Q000502:physiology*; D016026:Bone Marrow Transplantation; D002241:Carbohydrates / Q000494:pharmacology; D002453:Cell Cycle; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006358:Hot Temperature; D006801:Humans; D006825:Hybridomas / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007108:Immune Tolerance; D007372:Interferons / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4; D007378:Interleukins / Q000494:pharmacology; D008213:Lymphocyte Activation; D010641:Phenotype; D050378:T-Lymphocytes, Regulatory / Q000378:metabolism*
D001685:Biological Factors; D002241:Carbohydrates; D007376:Interleukin-2; D007378:Interleukins; D015847:Interleukin-4; D007372:Interferons