Epithelial dendritic cells in pathological human oral tissues.
Epithelial dendritic cells (EDC) were examined in human oral tissues with non-specific keratosis, lichen planus and squamous cell carcinoma. Acetone-fixed frozen sections were stained using an indirect immunoperoxidase technique and monoclonal antibodies to the human CD1 thymocyte (OKT6) and HLA-DR antigens. Significantly more T6+ and DR+ EDC were present in lichen planus tissues than normal controls, tissues with non-specific keratosis and the epithelial overlying/adjacent to squamous cell carcinomas, the latter tissues having comparable numbers of both T6+ and DR+ EDC. By contrast, significantly fewer T6+ EDC and significantly more DR+ cells were present in the invasive epithelium of squamous cell carcinomas than the overlying/adjacent epithelium of carcinomas, the non-specific keratosis group and the normal tissues. 23-60% of pathological tissues had either focal or general DR+ reactivity in keratinocytes, but there was no correlation between the density of T6+ or DR+ EDC and the keratinocyte DR status of the tissues. The results suggest that immunological enhancement occurs in lichen planus and possibly immunological impairment may characterize invasive squamous cell carcinoma.
Pitigala-Arachchi
A
A
Department of Oral Medicine, Surgery & Pathology, University of Bristol, England.
Crane
I J
IJ
Scully
C
C
Prime
S S
SS
D002294:Carcinoma, Squamous Cell / Q000473:pathology; D003713:Dendritic Cells / Q000276:immunology / Q000473:pathology*; D004817:Epidermis / Q000276:immunology / Q000473:pathology; D004848:Epithelium / Q000473:pathology; D006684:HLA-DR Antigens / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D007633:Keratins; D007801:Langerhans Cells / Q000473:pathology; D007972:Leukoplakia, Oral / Q000473:pathology; D008010:Lichen Planus / Q000473:pathology; D009059:Mouth Diseases / Q000473:pathology*; D009061:Mouth Mucosa / Q000276:immunology / Q000473:pathology*; D009062:Mouth Neoplasms / Q000473:pathology*
D006684:HLA-DR Antigens; D007633:Keratins