Effect of liposome encapsulation on antigen presentation in vitro. Comparison of presentation by peritoneal macrophages and B cell tumors.
We have studied the effect of liposome encapsulation of pigeon cytochrome-c (PCC) on its processing and presentation by peritoneal exudate cells and B-cell hybridomas to antigen specific T cells in vitro. Encapsulation of PCC in liposomes modestly increased the presentation when the macrophage was the presenting cell but totally eliminated presentation when the B cell hybridoma was the presenting cell as determined by the IL-2 response. Using radiolabeled PCC, the increased presentation by the macrophage was correlated with an increase in the uptake and rate of processing of the liposomal antigen. The elimination of presentation by the B cell was due to the inability of this cell type to internalize and degrade the encapsulated PCC. The results support the concept that the macrophage is the primary cell type involved in the initial stages of an immune response to a liposome encapsulated protein Ag in vivo.
Dal Monte
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Department of Pharmacy and Pharmaceutical Chemistry, University of California, San Francisco 94143-0446.
Szoka
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FC
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology* / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002460:Cell Line; D010856:Columbidae; D003574:Cytochrome c Group / Q000276:immunology / Q000378:metabolism; D000939:Epitopes / Q000276:immunology; D007700:Kinetics; D008081:Liposomes / Q000276:immunology* / Q000378:metabolism; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D010529:Peritoneal Cavity; D012995:Solubility; D013601:T-Lymphocytes / Q000276:immunology; D014407:Tumor Cells, Cultured / Q000276:immunology* / Q000378:metabolism
D003574:Cytochrome c Group; D000939:Epitopes; D008081:Liposomes