Vital fluorescent staining of human endothelial cells, fibroblasts, and monocytes: assessment of surface morphology.
Vital fluorescent staining of human endothelial cells, fibroblasts, and monocytes seeded on a variety of surfaces has been carried out to develop a method for studying cell growth and interactions on viable cells. Using special fluorochrome markers and monoclonal antibodies, it was possible to differentiate different cell types as they grew on polymer surfaces similar to ones that are presently used on artificial heart valves and vascular grafts.
Crawford
J M
JM
Division of Cardiac Surgery, Brigham and Women's Hospital, Boston, Massachusetts 02115.
Milford
E
E
Case
K
K
Braunwald
N S
NS
D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D003584:Cytological Techniques; D004730:Endothelium, Vascular / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology; D005456:Fluorescent Dyes; D006801:Humans; D009000:Monocytes / Q000166:cytology; D013194:Staining and Labeling; D014471:Umbilical Veins / Q000166:cytology
D000911:Antibodies, Monoclonal; D005456:Fluorescent Dyes