A common epitope on major allergens from non-biting midges (Chironomidae).
A synthetic peptide corresponding to sequence 91-101 of the Chironomus thummi thummi haemoglobins (Chi t I) components III and IV was used to investigate binding and cross-reactivity with polyclonal human IgE and rabbit IgG antibodies and murine IgG1 subclass monoclonal antibodies (MABs). The synthetic peptide reacted with antibodies from all three mammals. The specificity of the reaction, especially that with IgE antibodies was shown by dose dependent inhibition with native Chi t I component III. Epitope(s) reacting with these antibodies were also found in haemoglobins from 14 of the 15 chironomid species analyzed. The synthetic peptide III/IV 91-101 enabled the identification of an important antigenic/allergenic determinant of the broadly distributed insect family Chironomidae. The antigenic potency of this synthetic peptide as shown by testing with human IgE, rabbit IgG and mouse MABs, and the widespread occurrence of the epitope in an identical or homologous sequence and/or superficial location, qualifies the peptide for therapeutic applications in medicine.
Mazur
G
G
Pneumologische Abteilung, Klinikum Grosshadern, Ludwig-Maximilians-Universität, Munich, F.R.G.
Baur
X
X
Modrow
S
S
Becker
W M
WM
D000485:Allergens / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002683:Chironomidae / Q000276:immunology*; D004175:Diptera / Q000276:immunology*; D000939:Epitopes / Q000032:analysis*; D006454:Hemoglobins / Q000276:immunology; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology; D011817:Rabbits
D000485:Allergens; D000911:Antibodies, Monoclonal; D000939:Epitopes; D006454:Hemoglobins; D007074:Immunoglobulin G; D010446:Peptide Fragments; D007073:Immunoglobulin E