[Antigenic determinants of the constant region of human immunoglobin kappa-chain detected by monoclonal antibodies].
Ten different monoclonal antibody (monAB) preparations reacting with human IgL chains of the kappa type have been obtained. Nine of the monAB interacted with the kappa-chain C domain, whereas only one monAB reacted with the V domain. It has been determined that monAB against the C domain react with three different epitopes. One epitope is expressed on intact Ig molecules as well as on isolated kappa-chains, whereas the other two epitopes are found only on isolated kappa-chains. The expression of these epitopes in 40 different myeloma kappa-chain preparations belonging to four various subgroups was studied. The level of this C domain epitope expression has been shown to depend on the variable subgroups of kappa-chains indicating a close association between V and C domains. This association leads to the alteration of antigenic activity of some C domain epitopes. The alterations are thought to be local because, as a rule, they involve only one of the three epitopes.
Arsen'eva
E L
EL
Bogacheva
G T
GT
Solomon
A
A
Rokhlin
O V
OV
D000911:Antibodies, Monoclonal; D000939:Epitopes / Q000032:analysis*; D006801:Humans; D007127:Immunoglobulin Constant Regions / Q000276:immunology*; D007145:Immunoglobulin kappa-Chains / Q000276:immunology*; D009101:Multiple Myeloma / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007127:Immunoglobulin Constant Regions; D007145:Immunoglobulin kappa-Chains
Antigennye determinanty postoiannogo domena kappa-tsepeĭ immunoglobulinov cheloveka, vyiavliaemye s pomoshch'iu monoklonal'nykh antitel.