Modulation of cell proliferation by protein proteinase inhibitors. A new analytical approach.
Human alpha 1-proteinase inhibitor (alpha 1-antitrypsin) and bovine pancreatic trypsin inhibitor both inhibit cell-surface proteolytic activity in human fibroblasts. At the inhibitory concentrations, these proteins also inhibit cellular proliferation, as assayed by a microassay based on spectrophotometry of fixed and stained cells. Despite testing a wide range of proteinase inhibitor concentrations, no evidence was found for mitogenic or growth-stimulatory activity. ovomucoid was inactive against cell-surface proteinase activity and did not inhibit cellular proliferation.
Scott
G K
GK
Department of Biochemistry, University of Auckland.
Tse
C A
CA
D002455:Cell Division / Q000187:drug effects*; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D011480:Protease Inhibitors / Q000494:pharmacology*; D013194:Staining and Labeling
D011480:Protease Inhibitors