[The ratio in the DNA content and protein mass of human cardiomyocytes].
DNA and total protein content were measured in human cardiac myocytes using cytophotometry of Feulgen-naphthol yellow stained cells isolated from the left ventricle. The percentage of DNA classes was 2%, 32%, 53%, 12% and less than 0.5% for diploids, tetraploids (both 4c and 2c X 2), octaploids (8c, 4c X 2 and 2c X 4), hexadecaploids (16c, 8c X 2 and 4c X 4) and 32c cells, respectively. Binuclear cells comprised about 65% of the myocytes; the main class was 4c X 2. A discrepancy between total protein content and gene dose has been pointed out. DNA level ratio in a number of myocytes of different ploidy were 2:4:8:16:32; the same ratio for total protein content was 2:3.5:6:11.4:25.5, respectively.
Vasil'eva
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IA
Aref'eva
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AM
D000328:Adult; D003592:Cytophotometry; D004247:DNA / Q000032:analysis*; D006801:Humans; D008297:Male; D009206:Myocardium / Q000032:analysis* / Q000166:cytology; D011123:Polyploidy; D011506:Proteins / Q000032:analysis*; D013194:Staining and Labeling / Q000379:methods
D011506:Proteins; D004247:DNA
Sootnoshenie soderzhaniia DNK i belkovoĭ massy kardiomiotsitov cheloveka.