Identification and characterization of an L1 family sequence with a very long open reading frame in the third intron of the human thymidylate synthase gene.
A long L1 repetitive sequence (3.6 kilobase pairs) was found in the third intron of the human thymidylate synthase gene. This L1 family sequence is unique in that it possesses the longest open reading frame (1.7 kilobase pairs) of all L1 family members identified in sequences associated with specific genes that have been cloned thus far. Furthermore, the amino acid sequence deduced from the open reading frame of the L1 sequence was found to be highly homologous (90%) to that encoded by a known human teratocarcinoma L1 RNA species, and to contain several blocks of sequences homologous to ones in RNA-dependent DNA polymerases of various origins.
Horie
N
N
School of Food and Nutritional Sciences, University of Shizuoka, Yada.
Nalbantoglu
J
J
Kaneda
S
S
Ayusawa
D
D
Seno
T
T
Takeishi
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003062:Codon; D004247:DNA / Q000648:ultrastructure; D006801:Humans; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D013940:Thymidylate Synthase / Q000235:genetics*
D003062:Codon; D012313:RNA; D004247:DNA; D013940:Thymidylate Synthase