title: Transcriptionally active chromatin can be selectively released by DNase I from Physarum polycephalum genome.
abstract: In a simple eukaryote Physarum polycephalum about 13% of the genome is transcribed into abundant cytoplasmic RNA as shown by S1 nuclease digestion of DNA-RNA hybrids. Mild digestion of isolated Physarum nuclei with DNase I liberates a fraction of chromatin 3.5-fold enriched in sequences hybridizing by Physarum poly(A)+ RNA. This fraction is similarly enriched in histone H4 and actin genes known to be actively transcribed in Physarum. High content (about 45%) of actively transcribed sequences in DNase-I-released fraction of Physarum chromatin makes it particularly well suited for studying the structural basis of transcriptional activation in eukaryotes.
authors: Institute of Biochemistry, Warsaw University, Poland.
authors: J
authors: J
authors: Fronk
authors: A
authors: A
authors: Jerzmanowski
authors: J
authors: J
authors: Wiśniewski
authors: M
authors: M
authors: Czupryn
authors: K
authors: K
authors: Toczko
authors: M L
authors: ML
authors: Wilhelm
authors: F X
authors: FX
authors: Wilhelm
mesh_terms: D000199:Actins / Q000235:genetics; D002843:Chromatin / Q000235:genetics*; D003593:Cytoplasm; D003850:Deoxyribonuclease I / Q000378:metabolism*; D006657:Histones / Q000235:genetics; D006824:Hybridization, Genetic; D010804:Physarum / Q000235:genetics*; D012313:RNA; D012333:RNA, Messenger; D014158:Transcription, Genetic
chemical_list: D000199:Actins; D002843:Chromatin; D006657:Histones; D012333:RNA, Messenger; D012313:RNA; D003850:Deoxyribonuclease I