A comparison of three different methods for the determination of estrogen receptors in human breast cancer.
Forty-seven cases of breast cancer were analyzed for estrogen receptors (ERs) by three different techniques: (1) a biochemical assay using isoelectric focusing in polyacrylamide gel; (2) a monoclonal ER immunocytochemical assay (ERICA, Abbott Laboratories Diagnostics Division), and (3) a polyclonal antiestradiol immunohistochemical technique using formalin-fixed tissue. A high level of agreement (85%) was noted between the biochemical assay and ERICA. The ERICA-processed slides showed minimal background staining and were easy to interpret. Compared with the biochemical assay, ERICA achieved a positive predictive value of 86% and a negative predictive value of 82%. In contrast, results from the polyclonal antiestradiol histologic technique did not correlate with biochemical results and showed positive and negative predictive values of 61% and 25%, respectively. The authors conclude that the ERICA technique should prove to be a valuable adjunct to traditional biochemical ER assays.
Cudahy
T J
TJ
Department of Pathology, Linköping University Hospital, Sweden.
Boeryd
B R
BR
Franlund
B K
BK
Nordenskjöld
B A
BA
D000180:Acrylic Resins; D000906:Antibodies / Q000276:immunology; D000911:Antibodies, Monoclonal; D001943:Breast Neoplasms / Q000032:analysis*; D003600:Cytosol / Q000032:analysis; D004967:Estrogens / Q000276:immunology; D005260:Female; D005782:Gels; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007525:Isoelectric Focusing; D011933:Reagent Kits, Diagnostic; D011960:Receptors, Estrogen / Q000032:analysis*; D013194:Staining and Labeling; D000099166:Polyacrylamides
D000180:Acrylic Resins; D000906:Antibodies; D000911:Antibodies, Monoclonal; D004967:Estrogens; D005782:Gels; D011933:Reagent Kits, Diagnostic; D011960:Receptors, Estrogen; D000099166:Polyacrylamides