Chromatin organization in rat testis nuclei. Flow cytometric detection of the morphological compaction.
The unusual histone composition of testicular cells generates changes in chromatin organization in order to allow the chromosomal pairing necessary for genetic recombination. Accessibility of testis nuclear DNA was determined by flow cytometry. The observed differences in staining between testis and liver nuclear chromatin, as well as the differences of perpendicular light scatter signal, correlate with alterations in protein composition with the chromatin reorganization.
Papa
S
S
Istituto di Scienze Morfologiche, Urbino, Italy.
Vitale
M
M
Rizzoli
R
R
Zini
N
N
Matteucci
A
A
Cecchini
T
T
Maraldi
N M
NM
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis* / Q000648:ultrastructure; D002843:Chromatin / Q000032:analysis* / Q000648:ultrastructure; D004996:Ethidium; D005434:Flow Cytometry; D007399:Interphase; D008099:Liver / Q000032:analysis / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D009696:Nucleic Acids / Q000032:analysis / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling; D013737:Testis / Q000032:analysis* / Q000648:ultrastructure
D002843:Chromatin; D009696:Nucleic Acids; D004996:Ethidium