Mallory's phloxine B-methylene blue-azure II stain emphasizes elastin and collagen bundles in epoxy embedded lung.
A version of Mallory's phloxine-methylene blue-azure II technique suitable for large epoxy sections is described. Phloxine B (C.I. 45410) and a yellow-green interference filter (546-548 nm transmission) combine to give high contrast monochrome images. By comparing light micrographs of lung parenchyma entirely unstained or stained only with phloxine B against electron micrographs of the same material, it is seen that phloxine B emphasizes essentially only elastin and collagen fiber bundles. The technique has produced images useful for investigating lung parenchyma architecture and micromechanics.
Oldmixon
E H
EH
Division of Pulmonary Medicine, Memorial Hospital of Rhode Island, Providence.
D000818:Animals; D001399:Azure Stains; D003094:Collagen / Q000032:analysis*; D004285:Dogs; D004549:Elastin / Q000032:analysis*; D010697:Eosine I Bluish; D004853:Epoxy Resins; D008168:Lung / Q000032:analysis* / Q000166:cytology; D008751:Methylene Blue; D008867:Microtomy; D013194:Staining and Labeling / Q000379:methods*
D001399:Azure Stains; D004853:Epoxy Resins; D003094:Collagen; D004549:Elastin; D010697:Eosine I Bluish; D008751:Methylene Blue