title: New Achromobacter collagenase and its immunological relationship with a vertebrate collagenase.
abstract: Evidence is presented that Achromobacter iophagus produces two distinct collagenases. Achromobacter collagenases A and B were separated by high-performance liquid chromatography from partially purified enzyme. The main collagenase, A (EC 3.4.24.8), which has been already described, was eluted in the region of molecular mass 110-90 kDa. A minor collagenase B eluted in the region of 320 kDa, although in SDS-gel electrophoresis the apparent molecular masses of its main active forms were estimated as 55 and 110 kDa. The specificities of collagenases A and B are different. Collagenase A splits in its synthetic substrate Pz-Pro-Leu-Gly-Pro-DArg the bond Leu-Gly, collagenase B does not split this substrate. Both collagenases split bonds Gln-Gly and Leu-Gly in synthetic peptides DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-DArg-OH and DNP-Pro-Leu-Gly-Ile-Ala-Gly-DArg-NH2, respectively. Collagenase B is twice as active as A on the native collagen type I. Both enzymes are inhibited by EDTA. The antibodies raised against the human tooth collagenase specifically inhibited the collagenase B, but did not influence the activity of collagenase A. These results indicate, to our knowledge for the first time, an immunological relationship between a bacterial and a vertebrate collagenase.
authors: Unité de chimie des protéines, Institut Pasteur, Paris, France.
authors: T T
authors: TT
authors: Nguyen
authors: J
authors: J
authors: Dumas
authors: V
authors: V
authors: Keil-Dlouha
mesh_terms: D000595:Amino Acid Sequence; D003804:Dentin / Q000201:enzymology*; D000939:Epitopes / Q000032:analysis; D006088:Gram-Negative Aerobic Bacteria / Q000201:enzymology*; D006801:Humans; D007106:Immune Sera; D007700:Kinetics; D003012:Microbial Collagenase / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D013045:Species Specificity; D013379:Substrate Specificity; D014733:Vibrio / Q000201:enzymology
chemical_list: D000939:Epitopes; D007106:Immune Sera; D003012:Microbial Collagenase