Bleomycin-induced DNA repair by Saccharomyces cerevisiae ATP-dependent polydeoxyribonucleotide ligase.
In contrast to ligase-deficient (cdc9) Saccharomyces cerevisiae, which did not rejoin bleomycin-induced DNA breaks, ligase-proficient (CDC9) yeast cells eliminated approximately 90% of DNA breaks within 90 to 120 min after treatment. Experimental conditions restricted enzymatic removal of the unusual 3'-phosphoglycolate termini in DNA cleaved by bleomycin and involved doses producing equivalent numbers of DNA breaks or doses producing equivalent killing.
Moore
C W
CW
Department of Radiation Biology and Biophysics, University of Rochester School of Medicine and Dentistry, New York 14642.
D001761:Bleomycin / Q000494:pharmacology*; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D011088:DNA Ligases / Q000378:metabolism*; D004260:DNA Repair; D011118:Polynucleotide Ligases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013997:Time Factors
D001761:Bleomycin; D004247:DNA; D011088:DNA Ligases; D011118:Polynucleotide Ligases