Characterization of a human ovarian carcinoma.
An epithelial ovarian carcinoma from a patient with progressive disease who had received combination chemotherapy was established as a cell line and characterized. The doubling time of the cell line was seven days. The subcutaneous inoculation with 10(6) cells into an athymic nude mouse produced a 5 X 5-mm nodule with a histology similar to that of the original tumor. The malignant epithelial cells were aneuploid and varied in chromosome numbers from 50 to 115; double minutes were present in 25% of the cells. Antibodies specific for keratin showed a dense filamentous keratin network within the cells. No estrogen receptors were identified by immunocytochemistry. A heterogeneous tumor population in the sixth passage was suggested by flow cytometric analysis. This cell line may be a useful in vitro model for studying the biology and mechanisms of radiation and/or chemotherapy resistance of ovarian carcinomas.
Rotmensch
J
J
Department of Obstetrics and Gynecology, University of Chicago, IL 60637.
Schwartz
J L
JL
Beckett
M A
MA
Grdina
D J
DJ
Toohill
P
P
Weischelbaum
R R
RR
D000818:Animals; D002460:Cell Line; D003536:Cystadenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D007633:Keratins / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009368:Neoplasm Transplantation; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D011960:Receptors, Estrogen / Q000378:metabolism; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology*
D011960:Receptors, Estrogen; D007633:Keratins