Calcium release from frog sarcoplasmic reticulum by an imidazolyl reagent.
Calcium is released from the isolated heavy sarcoplasmic reticulum (SR) of frog skeletal muscle upon application of 0.1-1 mM diethylpyrocarbonate (DEP, an imidazolyl reagent). The Ca-ATPase activity of SR was suppressed by 20% in the presence of 1 mM DEP. More than 1 mM of free magnesium ion or 5 microM ruthenium red eliminated the effect of DEP on calcium release but not on Ca-ATPase activity. A plausible site of DEP action is on the calcium channel.
Aoki
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T
Department of Physiology, Nagoya City University Medical School, Japan.
Oba
T
T
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D004047:Diethyl Pyrocarbonate / Q000494:pharmacology*; D005561:Formates / Q000494:pharmacology*; D008274:Magnesium / Q000494:pharmacology; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D011892:Rana catesbeiana; D012430:Ruthenium Red / Q000494:pharmacology; D012519:Sarcoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism*
D005561:Formates; D012430:Ruthenium Red; D000252:Calcium-Transporting ATPases; D008274:Magnesium; D004047:Diethyl Pyrocarbonate; D002118:Calcium