title: Molecular evolution of the human and simian immunodeficiency viruses.
abstract: Molecular evolution and phylogeny of different human immunodeficiency virus type 1 (HIV1) strains, of a type 2 (HIV2) strain, and of two simian immunodeficiency viruses (SIVAGM and SIVMAC) have been studied by comparing the nucleotide sequences of the two regions of their pol genes which encode the reverse transcriptase (RT) and endonuclease/integrase (EN). The analyses show that the different HIV 1s form one cluster (HIV1 group) and that the SIVs and HIV2 form another (HIV2 group). When the entire genomes of a HIV1, a HIV2, and the two SIVs were compared, the SIVAGM showed a unique pattern of mutation accumulations; that is, the SIVAGM has accumulated more nonsynonymous changes than synonymous changes in the RT and EN regions after its recent divergence from SIVMAC-142, and, furthermore, it has a deletion of approximately 350 bp in the region between the pol and env genes. The SIVAGM was apparently derived from cell cultures infected with a macaque isolate, SIVMAC-251. The contamination provides an opportunity to measure the maximum rate of evolution in the SIVAGM by comparing its DNA sequence to those of SIVMAC-251 and SIVMAC-142. The analysis shows that the rates are given approximately by (1.95 +/- 1.37) x 10(-3)/site/year for one SIVAGM sequence and (5.18 +/- 2.25) x 10(-3)/site/year for another.
authors: University of Illinois, Urbana-Champaign 61820.
authors: S
authors: S
authors: Yokoyama
mesh_terms: D001483:Base Sequence; D005075:Biological Evolution; D004279:DNA, Viral; D004706:Endodeoxyribonucleases / Q000235:genetics; D005814:Genes, Viral; D019427:HIV Integrase; D015497:HIV-1 / Q000145:classification / Q000201:enzymology / Q000235:genetics; D015498:HIV-2 / Q000145:classification / Q000201:enzymology / Q000235:genetics; D009154:Mutation; D010802:Phylogeny; D012194:RNA-Directed DNA Polymerase / Q000235:genetics; D012190:Retroviridae / Q000145:classification / Q000201:enzymology / Q000235:genetics; D012191:Retroviridae Proteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D015302:Simian Immunodeficiency Virus / Q000145:classification / Q000201:enzymology / Q000235:genetics; D014790:Visna-maedi virus / Q000145:classification / Q000201:enzymology / Q000235:genetics
chemical_list: D004279:DNA, Viral; D012191:Retroviridae Proteins; D019427:HIV Integrase; D012194:RNA-Directed DNA Polymerase; D004706:Endodeoxyribonucleases; C058608:endonuclease-integrase