Effects of serum and serum-derived factors on the growth and production of alpha-fetoprotein and albumin by human hepatoma cell lines.
A serum-free culture system of human hepatoma cell lines (HuH-6 and HuH-7) was used to investigate the activity of bovine serum (BS) and of serum-derived factors on the growth and production of alpha-fetoprotein (AFP) and albumin. At higher concentrations, dialyzed BS was inhibitory to the growth of HuH-6 and caused reduction of the level of AFP production by the cells. AFP and albumin levels in HuH-6 and HuH-7 were reduced or unchanged by fetuin, bovine serum albumin (BSA) and transferrin (TF), although no cytotoxicity was shown by any of them. Commercial preparations of platelet-derived growth factor exhibited cytotoxicity to HuH-6 and HuH-7 and induced a decrease of AFP and albumin levels in a dose-dependent manner. Transforming growth factor (TGF-beta) exhibited no cytotoxicity to HuH-6. AFP levels in HuH-6 were unchanged with 1000 pg/ml TGF-beta, but albumin levels were decreased. TGF-beta at a concentration of 1000 pg/ml was cytotoxic to HuH-7 and AFP levels were a little increased. Albumin levels, however, were unchanged. Following exposure to cycloheximide, AFP and albumin levels in HuH-6 were inhibited.
Tokiwa
T
T
Division of Pathology, Okayama University Medical School, Japan.
Kusaka
Y
Y
Muraoka
A
A
Sato
J
J
D000418:Albumins / Q000096:biosynthesis*; D001790:Blood Physiological Phenomena; D001798:Blood Proteins / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D014168:Transferrin / Q000494:pharmacology; D015291:Transforming Growth Factors / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D000509:alpha-Fetoproteins / Q000096:biosynthesis* / Q000494:pharmacology
D000418:Albumins; D001798:Blood Proteins; D003470:Culture Media; D006133:Growth Substances; D010982:Platelet-Derived Growth Factor; D014168:Transferrin; D000509:alpha-Fetoproteins; D012710:Serum Albumin, Bovine; D015291:Transforming Growth Factors; D003513:Cycloheximide