Localization of a biologically important epitope on toxic-shock-syndrome toxin-1.
A monoclonal antibody, designated B-14, inhibits the nonspecific T lymphocyte mitogenicity of toxic-shock-syndrome toxin-1 (TSST-1), and the antibody binds to an internal cyanogen bromide (CNBr) fragment (Mr, 14,000) of the toxin. The epitope recognized by B-14 was further localized to include a decapeptide at the NH2-terminus of the CNBr fragment. The decapeptide inhibited the ELISA and western blot reactivity of B-14 with TSST-1, although it was approximately 10,000-fold less effective than the native toxin. The peptide also inhibited the capacity of B-14 to block TSST-1-induced mitogenicity. A conjugate, consisting of decapeptide4-ovalbumin, was used to hyperimmunize three rabbits. Serum from these rabbits reacted specifically with intact TSST-1 in ELISA and western blots and partially neutralized toxin mitogenicity; however, the serum did not prevent fever and enhancement of susceptibility to endotoxin shock typically seen in rabbits after administration of TSST-1.
Murphy
B G
BG
Department of Microbiology, University of Minnesota Medical School, Minneapolis 55455-0312.
Kreiswirth
B N
BN
Novick
R P
RP
Schlievert
P M
PM
D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D001427:Bacterial Toxins; D001681:Biological Assay; D004768:Enterotoxins / Q000032:analysis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D007118:Immunoassay; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis; D011817:Rabbits; D013211:Staphylococcus aureus; D018089:Superantigens
D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D004768:Enterotoxins; D000939:Epitopes; D010455:Peptides; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal