Heterogeneity of keratin expression in epithelial tumor cells of adenolymphoma in paraffin sections.
Immunohistochemical expressions of keratin polypeptides detected by monoclonal antibodies were described in tumor cells of adenolymphoma, and the possibility of intercalated duct and ductal basal cells in the salivary glands being the progenitors was discussed. Basal cells in the tumor showed positive staining for keratin nos. 8, 13, 16, 18 and 19 detecting for monoclonal keratin antibodies (PKK 1, K 4.62, K 8.12, K 8.13), columnar tumor cells displayed strongly positive reactions with RPN 1164 and K4.62 suggesting keratin nos. 8 and 19. Great heterogeneity of distribution for keratin polypeptides was displayed by epithelial cells of adenolymphoma. Intercalated duct cells of normal salivary glands reacted with RPN 1164, RPN 1165, K 4.62 and K 8.13 monoclonal antibodies, which indicates the presence of keratins 8 and 19; and ductal basal cells reacted with PKK 1, K 4.62 and K 8.12, suggesting nos. 8, 13, 16, 18 and 19 keratins. Distribution of involucrin was variable in tumor epithelium of adenolymphoma, and was negative in the normal gland. The immunohistochemical distribution of keratin types between basal tumor cells of adenolymphoma and ductal basal cells of the normal salivary gland was compared.
Orito
T
T
Department of Oral Surgery, Asahi University School of Dentistry, Gifu, Japan.
Shinohara
H
H
Okada
Y
Y
Mori
M
M
D000235:Adenolymphoma / Q000032:analysis / Q000378:metabolism* / Q000473:pathology; D000911:Antibodies, Monoclonal / Q000276:immunology; D004848:Epithelium / Q000032:analysis / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D010232:Paraffin; D012468:Salivary Gland Neoplasms / Q000032:analysis / Q000378:metabolism* / Q000473:pathology; D012469:Salivary Glands / Q000032:analysis / Q000166:cytology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D007633:Keratins; D010232:Paraffin