A comparison of the effects of various stimulatory agents on t-PA secretion by normal and malignant cell lines.
Succinyl con A and acetyl con A both stimulated epithelial cells to produce similar yields of tissue plasminogen activator (t-PA) to those previously obtained with native con A. However, unlike con A, the derivatized lectins did not adversely affect cell morphology and viability, and cells treated with succinyl con A could secrete t-PA for a prolonged period. Con A and the two derivatives produced similar morphological effects in Bowes melanoma cells, but t-PA production was not increased. Elevated cyclic nucleotide concentrations did not affect t-PA production from epithelial cells, but calcium ionophore treatment generated t-PA yields similar to those obtained with lectins. Azacytidine, which enhanced t-PA production from epithelial cells, did not increase yields from Bowes melanoma cells, and also sodium butyrate, reported to increase t-PA yields from human endothelial cells, had no effect on either cell line.
Clarke
J
J
Division of Biologics, PHLS-Centre for Applied Microbiology and Research, Salisbury, Wilts, UK.
Griffiths
B
B
Melling
J
J
Ling
R
R
Atkinson
T
T
Electricwala
A
A
D001374:Azacitidine / Q000494:pharmacology; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D000001:Calcimycin / Q000494:pharmacology; D002460:Cell Line; D003208:Concanavalin A / Q000031:analogs & derivatives / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D037102:Lectins / Q000494:pharmacology; D009712:Nucleotides, Cyclic / Q000494:pharmacology; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D010959:Tissue Plasminogen Activator / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D002087:Butyrates; D037102:Lectins; D009712:Nucleotides, Cyclic; D020148:Butyric Acid; D003208:Concanavalin A; D000001:Calcimycin; C019558:succinylconcanavalin A; D012313:RNA; C030718:acetylated concanavalin A; D004247:DNA; D010959:Tissue Plasminogen Activator; D001374:Azacitidine