[Isolation of alpha2-macroglobulin from human plasma using combination of pseudoligand affinity chromatography and gel filtration].
Combination of two procedures--pseudoligand affinity chromatography on Cibacron blue agarose and usual gel filtration on TSK HW-60 enabled to separate successfully blood plasma alpha 2-macroglobulin from immunoglobulin M, haptoglobins and low molecular weight proteins. The preparation of alpha 2-macroglobulin obtained exhibited high inhibitory activity towards proteases.
Smorodin
E P
EP
Arukaévu
Kh E
KhE
D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D006801:Humans; D012685:Sepharose / Q000031:analogs & derivatives; D000511:alpha-Macroglobulins / Q000302:isolation & purification*
D000511:alpha-Macroglobulins; C017842:cibacron blue-sepharose; D012685:Sepharose
Vydelenie alpha2-makroglobulina plazmy krovi cheloveka kombinatsieĭ metodov psevdoligandnoĭ affinnoĭ khromatografii i gel'-fil'tratsii.