Monoclonal antibody that defines the prostate specific antigen.
A monoclonal antibody to a protein with a single band in the 30-kD region was obtained from fusion of Balb/C mouse spleen cells immunized with epithelial fractions of BPH, with the myeloma cell line P3 x 63 Ag8 6.5.3 by standard procedures. This antigen was characterized in immunobinding studies with various cellular and target antigens and in immunoperoxidase staining.
Donn
F
F
Department of Urology, Marienhospital, Hamburg, Federal Republic of Germany.
Bruns
T
T
von Meyerinck
L
L
Schulz
M
M
Becker
W M
WM
Becker
H
H
Klosterhalfen
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000951:Antigens, Neoplasm / Q000032:analysis / Q000276:immunology*; D014408:Biomarkers, Tumor / Q000032:analysis / Q000276:immunology*; D015153:Blotting, Western; D002846:Chromatography, Affinity; D005455:Fluorescent Antibody Technique; D006801:Humans; D006825:Hybridomas; D015151:Immunoblotting; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011467:Prostate / Q000276:immunology*; D017430:Prostate-Specific Antigen; D011471:Prostatic Neoplasms / Q000276:immunology*; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D017430:Prostate-Specific Antigen