[Isolation of Yersinia enterocolitica in various conditions of cultivation].
The rates of Yersinia isolation from 156 samples of pathologic material cultivated by two methods, the routine one, with the selection of Yersinia from a microbial association by prolonged cooling of the examined material in slightly alkaline buffer and multiple inoculations into Endo's medium, and the technique suggested by the authors--a rapid method based on the treatment of the biologic material with a concentrated alkaline-salt mixture at 4 degrees C for 2 days and a single inoculation into inhibiting selective medium. Use of the rapid method has increased the isolation rate of Yersinia 4-fold, cut down the time of the investigation 2-3-fold, reduced the cost and simplified the technique of analysis.
Maganchuk
V P
VP
Bobrova
S E
SE
Golovnia
G M
GM
D000208:Acute Disease; D003470:Culture Media; D006801:Humans; D008278:Magnesium Sulfate / Q000494:pharmacology; D010710:Phosphates / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D017680:Potassium Compounds; D012965:Sodium Chloride / Q000494:pharmacology; D015009:Yersinia Infections / Q000175:diagnosis / Q000382:microbiology*; D015008:Yersinia enterocolitica / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*
D003470:Culture Media; D010710:Phosphates; D017680:Potassium Compounds; D012965:Sodium Chloride; D008278:Magnesium Sulfate; C013216:potassium phosphate; D011188:Potassium; C018279:sodium phosphate
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