title: Capped poly(A) leaders of variable lengths at the 5' ends of vaccinia virus late mRNAs.
abstract: Evidence for capped poly(A) leaders of variable lengths located immediately upstream of the translation initiation codon was obtained by direct analyses of a major late mRNA species. A decapping-recapping method was used to specifically substitute a radioactively labeled phosphate for an unlabeled one within the cap structure. RNase H-susceptible sites were made by hybridizing synthetic oligodeoxyribonucleotides to the mRNA encoding a late major structural protein of 11 kilodaltons. Sequences of the type m7G(5')pppAmp (Ap)nUpG. . ., where n varies from a few to more than 40 nucleotides, were deduced by analysis of the length and sequence of RNase H, RNase T1, and RNase U2 digestion products.
authors: Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
authors: B Y
authors: BY
authors: Ahn
authors: B
authors: B
authors: Moss
mesh_terms: D001345:Autoradiography; D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D004722:Endoribonucleases; D006367:HeLa Cells; D006801:Humans; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000032:analysis*; D012313:RNA / Q000032:analysis*; D012315:RNA Caps / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D016914:Ribonuclease H; D006163:Ribonuclease T1; D014158:Transcription, Genetic; D014616:Vaccinia virus / Q000235:genetics*
chemical_list: D012315:RNA Caps; D012333:RNA, Messenger; D012367:RNA, Viral; D011061:Poly A; D012313:RNA; D004722:Endoribonucleases; D016914:Ribonuclease H; D006163:Ribonuclease T1