Caldesmon150, caldesmon77 and skeletal muscle troponin T share a common antigenic determinant.
Caldesmon and troponin are known to control actin-myosin interaction in smooth and striated muscle. We showed that polyclonal antibodies to smooth muscle caldesmon150 cross-react with non-muscle caldesmon77 and with skeletal muscle troponin T. The epitope recognized by caldesmon antibodies is located in the CB 2 peptide [residues 71-151] of troponin T. It is supposed that the observed cross-reactivity is due to the common structure of the tropomyosin-binding site of caldesmon and troponin T.
Shirinsky
V P
VP
Institute of Experimental Cardiology, USSR Cardiology Research Center, USSR Academy of Medical Sciences, Moscow.
Biryukov
K G
KG
Vorotnikov
A V
AV
Gusev
N B
NB
D000818:Animals; D000918:Antibody Specificity; D002148:Calmodulin-Binding Proteins / Q000276:immunology*; D002417:Cattle; D002645:Chickens; D002918:Chymotrypsin; D003488:Cyanogen Bromide; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology*; D015151:Immunoblotting; D008970:Molecular Weight; D009130:Muscle, Smooth / Q000032:analysis; D009132:Muscles / Q000032:analysis*; D010446:Peptide Fragments / Q000276:immunology; D011817:Rabbits; D051381:Rats; D014336:Troponin / Q000276:immunology*; D020107:Troponin T
D002148:Calmodulin-Binding Proteins; D000939:Epitopes; D010446:Peptide Fragments; D014336:Troponin; D020107:Troponin T; D002918:Chymotrypsin; D003488:Cyanogen Bromide