[Determination of the O antigen of Shigella sonnei by a competitive immunoenzyme method].
A technique for immunoenzymatic diagnosis of dysentery by Shigella sonnei O-antigen was developed. For induction of antibodies to O-antigen rabbits were immunized by intravenous administration of a commercial antidysentery vaccine. Specific antibodies to O-antigen belonging to class G immunoglobulins and not binding to O-antigens of Sh. flexneri and Salmonella typhimurium were obtained. beta-Lactamase of Bacillus licheniformis 749/c was used as a marker enzyme in the immunoenzymatic assay. To increase the sensitivity, beta-lactamase molecules were preliminarily linked with glutaric aldehyde into oligomers. Conjugates of Sh. sonnei O-antigen with the oligomers of B. licheniformis 749/c beta-lactamase were prepared with the periodate method by oxidizing O-antigen. The conjugate was used in competing solid phase immunoenzymatic assay for determination of Sh. sonnei O-antigen in blood serum of patients with dysentery. The sensitivity of the assay is 0.5-1 ng per 1 ml of O-antigen.
Voronov
A V
AV
Sazykin
A Iu
AIu
Vengerov
Iu Iu
IuIu
Serebriakov
M Iu
MIu
Malov
V A
VA
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D000942:Antigens, Bacterial / Q000032:analysis*; D001428:Bacterial Vaccines / Q000276:immunology; D002138:Calibration; D004306:Dose-Response Relationship, Immunologic; D004405:Dysentery, Bacillary / Q000175:diagnosis; D006801:Humans; D007114:Immunization; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D019081:O Antigens; D011817:Rabbits; D012764:Shigella sonnei / Q000276:immunology*; D013997:Time Factors
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D007074:Immunoglobulin G; D019081:O Antigens
Opredelenie O-antigens Shigella sonnei konkurentnym immunofermentnym metodom.