The androgen-dependent rat prostate protein, probasin, is a heparin-binding protein that co-purifies with heparin-binding growth factor-1.
Rat prostate extracts contain an abundant 20-22 kilodalton heparin-binding protein with near identical chromatographic properties, but only 0.2-1% of the mitogenic activity, of bovine brain heparin-binding growth factor-1 (acidic fibroblast growth factor). Amino terminal amino acid sequence (met-met-thr-asp-lys-asn-leu-lys-lys-lys-ile-glu-gly-asn-trp-arg-thr-val -tyr- leu-ala-ala-ser-?-val-glu-lys-ile-asn-glu-gly-ser-pro) and immunochemical analysis revealed that the protein is identical to the androgen-dependent protein "probasin".
Matuo
Y
Y
Department of Endocrinology, Kagawa Medical School, 1750, Japan.
Adams
P S
PS
Nishi
N
N
Yasumitsu
H
H
Crabb
J W
JW
Matusik
R J
RJ
McKeehan
W L
WL
D000595:Amino Acid Sequence; D000727:Androgen-Binding Protein / Q000302:isolation & purification* / Q000494:pharmacology; D000818:Animals; D001681:Biological Assay; D002455:Cell Division / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D016220:Fibroblast Growth Factor 1; D006133:Growth Substances / Q000302:isolation & purification*; D006493:Heparin / Q000302:isolation & purification*; D008297:Male; D008934:Mitogens; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011467:Prostate / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D014407:Tumor Cells, Cultured
D000727:Androgen-Binding Protein; D006133:Growth Substances; D008934:Mitogens; C027519:probasin; D016220:Fibroblast Growth Factor 1; D006493:Heparin