[Preliminary application of the immunogold-silver staining technique in diagnosing thromboangiitis obliterans].
In this paper we reported the preliminary application of immunogold-silver staining (IGSS) technique in immunopathological diagnosis of thromboangiitis obliterans (TAO). We used IGSS technique to detect the immune complexes deposit in the vascular walls (18 cases) and the anti-vascular autoantibody in serum (28 cases) of TAO patients. The immune complexes deposited in tissue were found in 100%, and the anti-vascular autoantibody in 86% of TAO patients. The results of IGSS technique were compared with that of immunofluorescent staining, immunoenzyme staining and avidin-biotin-peroxidase complex staining techniques. It was demonstrated that both the sensitivity and specificity of IGSS technique were significantly higher than those of other techniques.
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D000328:Adult; D000936:Antigen-Antibody Complex / Q000032:analysis; D001323:Autoantibodies / Q000032:analysis; D006046:Gold; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D012834:Silver; D013194:Staining and Labeling / Q000379:methods; D013919:Thromboangiitis Obliterans / Q000175:diagnosis*
D000936:Antigen-Antibody Complex; D001323:Autoantibodies; D012834:Silver; D006046:Gold