Characterization of a new endothelial cell growth factor (f-ECGF) partially purified from the supernatant of human fibroblast cells.
A new endothelial cell growth factor (f-ECGF) was partially purified from the cultured medium of human fibroblast cells of embryonic lungs. The partially purified f-ECGF induced neovascularization in rabbit cornea. It showed a selective growth stimulatory activity on the endothelial cells in vitro, whereas acidic- and basic-fibroblast growth factors (a- and b-FGFs) showed a broad spectrum of growth stimulation among tissues or cells. f-ECGF did not compete with the binding of a-FGF to the cell surface receptor in HEP-G2 hepatoblastoma cell lines. These results indicated that f-ECGF is a new endothelial cell growth factor distinct from a- and b-FGFs which are known to be potent endothelial cell growth factors.
Satoh
T
T
Department of Cell Biology, Tohoku-University, Sendai, Japan.
Kan
M
M
Masuko
K
K
Obinata
M
M
D000818:Animals; D002417:Cattle; D002460:Cell Line; D004730:Endothelium, Vascular / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D005346:Fibroblast Growth Factors / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D005347:Fibroblasts / Q000032:analysis; D006801:Humans; D009389:Neovascularization, Pathologic; D011817:Rabbits; D017468:Receptors, Fibroblast Growth Factor; D011975:Receptors, Mitogen / Q000378:metabolism; D040262:Receptors, Vascular Endothelial Growth Factor
D017468:Receptors, Fibroblast Growth Factor; D011975:Receptors, Mitogen; D005346:Fibroblast Growth Factors; D040262:Receptors, Vascular Endothelial Growth Factor