[Isolation of mRNA from protoplasts of spore-forming bacteria and study of its translation products].
Total RNA was isolated from the protoplasts of Bacillus intermedius 7P which actively produced alkaline exocellular RNAse. A fraction of polyadenylated RNA was isolated from this RNA using chromatography on poly(U) Sepharose. The total RNA and poly(A) +RNA were translated in Xenopus laevis oocytes. Bacterial exocellular RNAse was identified among the products of translation by means of electrophoretic and immunological analyses. The mRNA of RNAse secreted by B. intermedius 7P was concluded to be polyadenylated.
Sharipova
F R
FR
Romakhina
E R
ER
Bagaeva
T V
TV
Balaban
N P
NP
Leshchinskaia
I B
IB
D000818:Animals; D001407:Bacillus / Q000235:genetics* / Q000502:physiology; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D007122:Immunoelectrophoresis; D009865:Oocytes / Q000378:metabolism; D014176:Protein Biosynthesis; D011523:Protoplasts / Q000378:metabolism*; D012329:RNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification*; D013171:Spores, Bacterial; D014982:Xenopus laevis / Q000235:genetics
D012329:RNA, Bacterial; D012333:RNA, Messenger
Poluchenie mRNK iz protoplastov sporobrazuiushchikh bakteriĭ i izuchenie produktov ee transliatsii.