Aspartame fails to facilitate pentylenetetrazol-induced convulsions in CD-1 mice.
Concentrations of plasma amino acids and brain monoamines as well as pentylenetetrazol-induced seizures were monitored in CD-1 mice treated with aspartame in acute oral doses from 0 to 2500 mg/kg. One hour after administration aspartame produced increases in plasma concentrations of phenylalanine and tyrosine and modest reductions in concentrations of brain serotonin and 5-hydroxyindole acetic acid. However, these effects of the sweetener had no influence on the convulsive dose fifty (CD50) of pentylenetetrazol. Moreover, aspartame failed to alter the percentage of mice exhibiting seizures when exposed to an approximate CD50 of pentylenetetrazol. Finally, aspartame had no effect on brain norepinephrine or dopamine concentrations. In sharp contrast to previously reported studies, these observations suggest that aspartame, given in heroic doses, does not alter the propensity to seizure activity in CD-1 mice. We conclude that changes in plasma amino acids and brain serotonin produced by large oral bolus doses of aspartame are insufficient to result in functional deficits which might have the capacity to facilitate pentylenetetrazol-induced seizures.
Dailey
J W
JW
Department of Basic Sciences, University of Illinois College of Medicine, Peoria 61656.
Lasley
S M
SM
Mishra
P K
PK
Bettendorf
A F
AF
Burger
R L
RL
Jobe
P C
PC
D000596:Amino Acids / Q000097:blood; D000818:Animals; D001218:Aspartame / Q000633:toxicity*; D001923:Brain Chemistry / Q000187:drug effects; D004151:Dipeptides / Q000633:toxicity*; D006897:Hydroxyindoleacetic Acid / Q000032:analysis; D008297:Male; D051379:Mice; D009638:Norepinephrine / Q000032:analysis; D010433:Pentylenetetrazole; D012640:Seizures / Q000139:chemically induced*; D012701:Serotonin / Q000032:analysis
D000596:Amino Acids; D004151:Dipeptides; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D010433:Pentylenetetrazole; D009638:Norepinephrine; D001218:Aspartame