title: Comparison of Corynebacterium parvum and Bordetella pertussis with Freund's complete adjuvant as immunopotentiators for beta-human chorionic gonadotropin linked to an atoxic fragment of tetanus toxin.
abstract: Corynebacterium parvum and Bordetella pertussis were compared with Freund's complete adjuvant (FCA) for their abilities to potentiate the immune response to haptenic beta-human chorionic gonadotropin covalently coupled to an atoxic 54,000-molecular-weight fragment of tetanus toxin (beta-hCG-TTII). The ability of each adjuvant to enhance production of antibodies to hCG in rabbits was measured by 125I-hCG radioimmunoassay. At sera dilutions of 1:10,000, analysis of variance for the 8-week postimmunization course showed that the mean 125I-hCG binding capacities of the C. parvum group was significantly greater overall than the B. pertussis group (P = .0002) and that the FCA-treated group had the greatest binding capacity overall (P less than .018). The mean binding capacities at 1:40,000 dilution again showed the FCA-treated group to have significantly higher anti-hCG titers overall (P less than .0015), with C. parvum potentiating a greater overall antibody response than B. pertussis (P = .001). These results indicate that FCA is the most efficacious of the three tested adjuvants in potentiating antibody production to the hapten component of beta-hCG-TTII. C. parvum was also effective at promoting an anti-beta-hCG response, although not to the same degree as FCA. B. pertussis had only minimal potentiating effect compared to FCA or C. parvum.
authors: Department of Biological Sciences, University of Idaho, Moscow.
authors: D C
authors: DC
authors: Covey
authors: C C
authors: CC
authors: Chang
authors: K A
authors: KA
authors: Laurence
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation; D001886:Bordetella pertussis / Q000276:immunology*; D006063:Chorionic Gonadotropin / Q000276:immunology*; D018997:Chorionic Gonadotropin, beta Subunit, Human; D005260:Female; D005620:Freund's Adjuvant / Q000494:pharmacology*; D010446:Peptide Fragments / Q000276:immunology*; D011425:Propionibacterium acnes / Q000276:immunology*; D011817:Rabbits; D013744:Tetanus Toxin / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D006063:Chorionic Gonadotropin; D018997:Chorionic Gonadotropin, beta Subunit, Human; D010446:Peptide Fragments; D013744:Tetanus Toxin; D005620:Freund's Adjuvant