Recombinant human interleukin-6 (IL-6/BSF-2/HSF) regulates the synthesis of acute phase proteins in human hepatocytes.
Recombinant human IL-6 (rhIL-6) is a potent inducer of the synthesis of acute phase proteins in adult human hepatocytes. A wide spectrum of acute phase proteins is regulated by this mediator. After labeling of rhIL-6 stimulated human hepatocytes with [35S]methionine acute phase protein synthesis was measured by immunoprecipitation. Serum amyloid A, C-reactive protein, haptoglobin, alpha 1-antichymotrypsin and fibrinogen were strongly induced (26-, 23-, 8.6-, 4.6- and 3.8-fold increases, respectively). Moderate increases were found for alpha 1-antitrypsin (2.7-fold) and alpha 1-acid glycoprotein (2.7-fold). RhIL-6 had no effect on alpha 2-macroglobulin, whereas fibronectin, albumin and transferrin decreased to 64, 56 and 55% of controls. In the cases of serum amyloid A, haptoglobin, alpha 1-antichymotrypsin, alpha 1-antitrypsin and alpha 1-acid glycoprotein, dexamethasone enhanced the action of rhIL-6. We conclude that rhIL-6 controls the acute phase response in human liver cells.
Castell
J V
JV
Biochemisches Institut, Universität Freiburg, FRG.
Gómez-Lechón
M J
MJ
David
M
M
Hirano
T
T
Kishimoto
T
T
Heinrich
P C
PC
D000209:Acute-Phase Proteins / Q000096:biosynthesis*; D000328:Adult; D002097:C-Reactive Protein / Q000096:biosynthesis; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005340:Fibrinogen / Q000096:biosynthesis; D006242:Haptoglobins / Q000096:biosynthesis; D006801:Humans; D007163:Immunosorbent Techniques; D015850:Interleukin-6; D007378:Interleukins / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism; D009961:Orosomucoid / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000494:pharmacology*; D000685:Serum Amyloid A Protein / Q000096:biosynthesis; D000514:alpha 1-Antichymotrypsin / Q000096:biosynthesis; D000515:alpha 1-Antitrypsin / Q000096:biosynthesis; D000511:alpha-Macroglobulins / Q000096:biosynthesis
D000209:Acute-Phase Proteins; D006242:Haptoglobins; D015850:Interleukin-6; D007378:Interleukins; D009961:Orosomucoid; D011994:Recombinant Proteins; D000685:Serum Amyloid A Protein; D000514:alpha 1-Antichymotrypsin; D000515:alpha 1-Antitrypsin; D000511:alpha-Macroglobulins; D003907:Dexamethasone; D005340:Fibrinogen; D002097:C-Reactive Protein; D008715:Methionine