title: Neutralizing monoclonal antibody specific for alpha-bungarotoxin: preparation and characterization of the antibody, and localization of antigenic region of alpha-bungarotoxin.
abstract: We prepared an alpha-bungarotoxin-specific monoclonal antibody that neutralizes the biological activity of the toxin in vivo. The antigenic determinant combining specifically with this antibody was determined on the basis of cross-reaction experiments using three other long neurotoxins and peptide fragments of alpha-bungarotoxin. The antigenic determinant was located on the peptide fragment containing S34-S35-R36-G37-K38, which forms a part of the expected site that binds to the acetylcholine receptor proteins.
authors: Department of Molecular Physiology, Tokyo Metropolitan Institute of Medical Science, Japan.
authors: R
authors: R
authors: Kase
authors: H
authors: H
authors: Kitagawa
authors: K
authors: K
authors: Hayashi
authors: K
authors: K
authors: Tanoue
authors: F
authors: F
authors: Inagaki
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D002038:Bungarotoxins / Q000032:analysis / Q000276:immunology*; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis*; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000032:analysis / Q000276:immunology; D011487:Protein Conformation; D012697:Serine Endopeptidases
chemical_list: D000911:Antibodies, Monoclonal; D002038:Bungarotoxins; D000939:Epitopes; D010446:Peptide Fragments; D012697:Serine Endopeptidases; C036813:lysyl endopeptidase