Evaluation of the rat tail model for estimating dermal absorption of lindane.
Dermal absorption of the insecticide lindane was determined following topical application of ring 14C-labeled lindane to the tail of Sprague-Dawley rats. The tail was tested as a practical alternative to the rat mid-dorsal (back) region, and the data obtained were compared to those with rat back and with those of rhesus monkeys in our previous reports. There was no significant difference between total percentage urinary 14C recovery for rats dosed on the tail with occlusive tail covers (52 +/- 6.2%; t1/2 = 2.7 d) compared to those with nonocclusive covers (55 +/- 4.4%; t1/2 = 2.9 d). Neither the total percentage urinary recovery nor the t1/2 values obtained for the rat tail and rat back models differed significantly. Carbon-14 activity was still detectable in urine samples taken after 72 d post-treatment. However, an extensive tissue analysis failed to demonstrate 14C activity persisting at 72 d, with the exception of trace levels detected in blood serum and tail tissue. Advantages of the rat tail model are highlighted.
Moody
R P
RP
Bureau of Chemical Hazards, Department of National Health and Welfare, Ottawa, Ontario, Canada.
Grayhurst
M
M
Ritter
L
L
D000287:Administration, Topical; D000818:Animals; D000882:Haplorhini; D001556:Hexachlorocyclohexane / Q000493:pharmacokinetics*; D006801:Humans; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012869:Skin Absorption; D013623:Tail
D001556:Hexachlorocyclohexane