Glycopeptides from murine teratocarcinoma cells: structure of the determinants recognised by Griffonia simplicifolia agglutinin I and by sera from patients with ovarian germ cell tumors.
High-molecular-weight glycopeptides synthesised by teratocarcinoma OTT6050 bear the binding site for Griffonia simplicifolia agglutinin I and are recognised by antibodies in the sera of patients with ovarian germ cell tumors. Digestion of the glycopeptides with endo-beta-D-galactosidase C abolished the lectin binding activity and the antigenic activity. Since the product of the enzymic digestion is alpha-D-Gal-(1----3)-D-Gal, it is concluded that the disaccharide structure is involved in the lectin binding site and the antigenic site.
Kamada
Y
Y
Department of Biochemistry, Faculty of Medicine, Kagoshima University, Japan.
Muramatsu
H
H
Muramatsu
T
T
Kawata
M
M
Sekiya
S
S
Takamizawa
H
H
D000818:Animals; D000912:Antibodies, Neoplasm / Q000276:immunology*; D000936:Antigen-Antibody Complex; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D000939:Epitopes / Q000032:analysis*; D005260:Female; D006020:Glycopeptides / Q000276:immunology / Q000302:isolation & purification*; D006026:Glycoside Hydrolases; D006384:Hemagglutination; D006801:Humans; D037102:Lectins; D051379:Mice; D010051:Ovarian Neoplasms / Q000276:immunology*; D037121:Plant Lectins; D013724:Teratoma / Q000032:analysis* / Q000276:immunology; D001616:beta-Galactosidase
D000912:Antibodies, Neoplasm; D000936:Antigen-Antibody Complex; D000939:Epitopes; D006020:Glycopeptides; C045475:Griffonia simplicifolia lectins; D037102:Lectins; D037121:Plant Lectins; D006026:Glycoside Hydrolases; C057030:endo-beta-D-galactosidase C; D001616:beta-Galactosidase