Differentiation of staphylococcal species and strains by ribosomal RNA gene restriction patterns.
Staphylococcal DNA was digested with endonucleases and probed with labelled ribosomal RNA (rRNA) from Escherichia coli. Reproducible restriction patterns containing between seven and 22 bands were obtained for seven different species of staphylococci. These profiles were species-specific with different strains of a particular species sharing an identical or similar restriction pattern. The results reported here indicate that rRNA gene restriction pattern analyses have an application in the taxonomy of staphylococci.
Thomson-Carter
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FM
Department of Medical Microbiology, University of Aberdeen Medical School, Foresterhill, UK.
Carter
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PE
Pennington
T H
TH
D015373:Bacterial Typing Techniques; D015139:Blotting, Southern; D005798:Genes, Bacterial; D012150:Polymorphism, Restriction Fragment Length; D015347:RNA Probes; D012329:RNA, Bacterial / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012339:RNA, Ribosomal, 28S / Q000235:genetics*; D015183:Restriction Mapping; D013045:Species Specificity; D013210:Staphylococcus / Q000145:classification* / Q000235:genetics
D015347:RNA Probes; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012339:RNA, Ribosomal, 28S