Convergence of serotonin-, enkephalin- and substance P-like immunoreactive afferent fibers onto cat medullary dorsal horn projection neurons: a triple immunocytochemical staining technique combined with the retrograde HRP-tracing method.
Convergence of serotonin (5-HT)-, enkephalin (ENK)-, and substance P (SP)-like immunoreactive (LI) afferent fibers onto cat medullary dorsal horn projection neurons was examined by retrogradely labeling trigeminothalamic neurons and immunocytochemically labeling 5-HT, ENK and SP fibers in the same tissue section using distinct chromogens. Convergence of 5-HT-, ENK- and SP-LI fibers upon single projection neurons was often observed in laminae I and V.
Tashiro
T
T
Department of Oral Anatomy (2nd Division), School of Dentistry, Hiroshima University, Japan.
Ruda
M A
MA
Satoda
T
T
Matsushima
R
R
Mizuno
N
N
D000818:Animals; D002415:Cats; D004745:Enkephalins / Q000378:metabolism*; D006735:Horseradish Peroxidase; D007150:Immunohistochemistry; D009475:Neurons, Afferent / Q000166:cytology / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism*; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*; D013373:Substance P / Q000378:metabolism*
D004745:Enkephalins; D012701:Serotonin; D013373:Substance P; D006735:Horseradish Peroxidase