Production of anti-HBsAg monoclonal antibodies.
The production of monoclonal antibodies against the HBsAg is reported. Balb/c mice immunized against a commercial vaccine were used. Upon fusion of spleen cells from an animal having a high titer with the SP2/0 myeloma cell line, we obtained 6 stable cell lines, all of the IgG1 subclass. They showed a wide range of specificities against the classical HBsAg subtypes. These monoclonal antibodies can be used as the basis for the development of new methods for the screening and study of the hepatitis B virus.
Vieira
J G
JG
Setor de Desenvolvimento Técnico (SDT), Laboratório Fleury, São Paulo, Brasil.
Nishida
S K
SK
da-Silva
S M
SM
Ferraz
M L
ML
Granato
C
C
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000939:Epitopes; D005260:Female; D006510:Hepatitis B Antibodies / Q000096:biosynthesis*; D006514:Hepatitis B Surface Antigens / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D014611:Vaccination
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens