Stromal cell-dependent growth of leukemic cells from murine erythroblastic leukemia.
Transplantable erythroblastic leukemia was induced by 300-rad irradiation of C3H mice. Conditions for in vitro growth of the leukemic cells were studied. None of interleukin-3, granulocyte/macrophage colony-stimulating factor and erythropoietin could support the growth of the cells in vitro. In contrast, the leukemic cells grew into a stroma-dependent cell line, ELM-D, in close contact with the stromal cell layer of 900-rad-irradiated long-term bone marrow culture. A stroma-independent cell line, termed ELM-I-1, was further established from the non-adherent population in the co-culture of the leukemic cells, ELM-D, with stromal cells. Reverse transcriptase activity was not detectable in ELM-D or ELM-I-1 cells. Studies on binding and cross-linking of 125I-erythropoietin showed that ELM-I-1 cells had erythropoietin receptors, and two major radiolabeled protein products with molecular weights of 120 kDa and 140 kDa were detected on sodium dodecyl sulfate/polyacrylamide gel electrophoresis under reducing conditions.
Itoh
K
K
Department of Biology, Faculty of Science, Niigata University.
Sasaki
R
R
Ono
K
K
Tezuka
H
H
Sakoda
H
H
Sawada
H
H
Hitomi
K
K
Nakane
H
H
Uchiyama
T
T
Uchino
H
H
D000818:Animals; D002455:Cell Division; D003115:Colony-Stimulating Factors / Q000302:isolation & purification; D003432:Cross-Linking Reagents; D004259:DNA-Directed DNA Polymerase / Q000032:analysis; D004921:Erythropoietin / Q000302:isolation & purification; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology*; D051379:Mice; D008809:Mice, Inbred C3H; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D014407:Tumor Cells, Cultured / Q000473:pathology
D003115:Colony-Stimulating Factors; D003432:Cross-Linking Reagents; D004921:Erythropoietin; D012194:RNA-Directed DNA Polymerase; D004259:DNA-Directed DNA Polymerase