Fever and acute phase reactants in the rat.
In rats synthesis of some acute phase reactants can be induced by a combination of corticosteroids and adrenaline. During fever both hormones show high plasma levels. We studied the effect of fever induced by intra-cerebroventricular (i.c.v.) injection of PGE2 on the acute phase response. Fever was continuously recorded and 24 h after induction acute phase reactant (APR) response was measured as indicated by the rise of alpha-macrofetoprotein (alpha M FP, alpha 2 macroglobulin of the rat). Controls received 0.9% saline i.c.v. Controls did not develop fever (dTmax less than or equal to 1 degree C) nor did they show significant APR response. The maximal rise in body temperature after PGE2 (2.6 +/- 0.7 degrees C) correlated significantly with the rise in alpha M FP concentration 24 h later. Adrenalectomy prevented the APR response completely but the magnitude of the fever reaction remained the same (2.1 +/- 0.3 degrees C). alpha-Blockade gave a smaller fever response but had no effect on the APR response. In alpha- and beta-blockade, fever response was normal but no APR response was obtained. Destroying the sympathetic nerve supply to the liver with 6-OH dopamine retarded the fever response but again APR response was not impeded. In order to differentiate between the role of fever as such and the effect of PGE2 on APR synthesis, we used heat exposure to induce hyperthermia in normal rats who showed an APR response comparable with that after i.c.v. PGE2. Pretreatment with sodium salicylate before inducing hyperthermia led to a variable rise in alpha M FP. Fever as such, without tissue injury, induces an APR response. The pathway to this effect probably involves circulating corticosterone and adrenaline, possibly via a beta-receptor mediated stimulation.
van Vugt
H
H
Department of Experimental Internal Medicine, Academic Medical Centre, University of Amsterdam, The Netherlands.
van Gool
J
J
Deutz
N E
NE
D000209:Acute-Phase Proteins / Q000096:biosynthesis*; D000210:Acute-Phase Reaction / Q000276:immunology; D000315:Adrenalectomy; D000818:Animals; D001262:Atenolol / Q000494:pharmacology; D001340:Autonomic Nerve Block; D003345:Corticosterone / Q000097:blood; D015232:Dinoprostone; D004837:Epinephrine / Q000097:blood; D005334:Fever / Q000139:chemically induced / Q000276:immunology*; D008099:Liver / Q000276:immunology; D008297:Male; D009638:Norepinephrine / Q000097:blood; D010643:Phenoxybenzamine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D000209:Acute-Phase Proteins; D010643:Phenoxybenzamine; D001262:Atenolol; D015232:Dinoprostone; D003345:Corticosterone; D009638:Norepinephrine; D004837:Epinephrine