Potentiation of the antiviral activity of poly r(A-U) by riboflavin, FAD and FMN.
The role of riboflavin (RFN), FAD or FMN in modulating the antiviral activity of poly r(A-U) was examined by the human foreskin fibroblast-vesicular stomatitis virus bioassay in which the concentrations of poly r(A-U) was fixed at 0.1 mM or 0.2 mM while the riboflavin, FAD or FMN concentration was varied to produce variable RFN (or FAD or FMN)/ribonucleotide ratios ranging from 1/16 to 2/1. Riboflavin, FAD and FMN tested individually did not exhibit any antiviral activity, while poly r(A-U) alone exhibited antiviral activity. When poly r(A-U) was combined with riboflavin, FAD or FMN, the antiviral activity was potentiated seven- to twelve-fold at RFN (or FAD or FMN)/ribonucleotide ratios in the region of 1/4.
Jamison
J M
JM
Department of Chemistry, Kent State University, Ohio 44242.
Flowers
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Kitareewan
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Krabill
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Tsai
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D003588:Cytopathogenic Effect, Viral; D004357:Drug Synergism; D005347:Fibroblasts; D005486:Flavin Mononucleotide / Q000494:pharmacology*; D005182:Flavin-Adenine Dinucleotide / Q000494:pharmacology*; D006801:Humans; D007372:Interferons / Q000096:biosynthesis; D008297:Male; D011063:Poly A-U / Q000494:pharmacology*; D012256:Riboflavin / Q000494:pharmacology*; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects* / Q000502:physiology; D010948:Viral Plaque Assay
D005182:Flavin-Adenine Dinucleotide; D011063:Poly A-U; D005486:Flavin Mononucleotide; D007372:Interferons; D012256:Riboflavin