title: The immune response and the eye. III. Anterior chamber-associated immune deviation can be adoptively transferred by serum.
abstract: After the anterior chamber (AC) injection of trinitrophenol-coupled (TNP) spleen cells, it is observed that systemic delayed-type hypersensitivity responses to TNP are inhibited by Ag-specific suppressor T cells. We recently reported that suppression is initiated by viable TNP-coupled T cells within the inoculum and upon further analysis we found that these cells have the surface phenotype of CD4+ Ts inducer cells. We report here that treatment of these TNP-T cells with cycloheximide or cytochalasin-B before to AC injection abolishes suppression, whereas treatment with 2000 rad radiation does not. This indicates that protein synthesis and secretion are required to initiate suppression but proliferation is not. Further, we demonstrate the adoptive transfer of suppression by serum of AC inoculated animals. Detection of the component in serum in adoptive transfer assays, however, requires removal of the spleen before AC injection. We establish that the material in serum is a Ts cell product (T suppressor-inducer factor) based on three criteria: it is Ag specific, genetically restricted, and reactive with a mAb that specifically identifies these molecules. These results suggest that the signal leaving the eye to induce suppression of delayed-type hypersensitivity is T cell derived and that molecules mediating immune regulation for this organ are made within the eye and transported via the serum to the spleen.
authors: Department of Ophthalmology, Washington University School of Medicine, St. Louis, MO 63110.
authors: T A
authors: TA
authors: Ferguson
authors: J D
authors: JD
authors: Hayashi
authors: H J
authors: HJ
authors: Kaplan
mesh_terms: D000818:Animals; D000867:Anterior Chamber / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D003513:Cycloheximide / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D000939:Epitopes / Q000032:analysis; D006023:Glycoproteins / Q000097:blood* / Q000235:genetics / Q000502:physiology; D007111:Immunity, Cellular; D007116:Immunization, Passive; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008819:Mice, Nude; D009363:Neoplasm Proteins; D013154:Spleen / Q000276:immunology; D013491:Suppressor Factors, Immunologic / Q000097:blood* / Q000235:genetics / Q000502:physiology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000637:transplantation; D014301:Trinitrobenzenes / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D006023:Glycoproteins; D009363:Neoplasm Proteins; D013491:Suppressor Factors, Immunologic; D014301:Trinitrobenzenes; C038588:serum immunosuppressive factor, human; D003571:Cytochalasin B; D003513:Cycloheximide