title: Characterization of the binding epitope of a monoclonal antibody to sulphatide.
abstract: An IgG1 monoclonal antibody, Sulph I, reacting with sulphatide (3'-sulphogalactosylceramide), was produced by immunizing Balb/c mice with that glycolipid coated on Salmonella minnesota bacterial membrane. Radioimmunodetection of the binding of the monoclonal antibody to structurally related glycolipids adsorbed to microtitre plates or chromatographed on thin-layer plates was used to determine its binding epitope. The antibody showed similar binding avidity to three sulphated glycolipids: sulphatide, sulpholactosylceramide and seminolipid. Lysosulphatide did bind the antibody, but, compared with sulphatide, 30 times more antigen was needed for half-maximal binding. Bis(sulphogangliotriosyl)ceramide and bis-sulphogangliotetraosylceramide did not bind the antibody. These results suggest that terminal galactose-3-O-sulphate and part of the hydrophobic region of the glycolipid are recognized by the Sulph I antibody.
authors: Department of Psychiatry and Neurochemistry, University of Gothenburg, Sweden.
authors: P
authors: P
authors: Fredman
authors: L
authors: L
authors: Mattsson
authors: K
authors: K
authors: Andersson
authors: P
authors: P
authors: Davidsson
authors: I
authors: I
authors: Ishizuka
authors: S
authors: S
authors: Jeansson
authors: J E
authors: JE
authors: Månsson
authors: L
authors: L
authors: Svennerholm
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D000915:Antibody Affinity; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D002554:Cerebrosides / Q000276:immunology*; D002855:Chromatography, Thin Layer; D000939:Epitopes / Q000276:immunology*; D005699:Galactosylceramides / Q000276:immunology*; D006017:Glycolipids / Q000276:immunology; D011863:Radioimmunoassay; D013329:Structure-Activity Relationship
chemical_list: D000911:Antibodies, Monoclonal; D002554:Cerebrosides; D000939:Epitopes; D005699:Galactosylceramides; D006017:Glycolipids