The induction of respiratory allergy in guinea-pigs following intradermal injection of trimellitic anhydride: a comparison with the response to 2,4-dinitrochlorobenzene.
Guinea-pigs injected intradermally with the known respiratory sensitiser trimellitic anhydride (TMA) developed high-titre antigen-specific homocytotropic (IgG1 and IgE) antibodies. Many of the sensitised animals responded to a challenge by inhalation with either free TMA or a TMA-protein conjugate with a change in respiratory rate, reflecting the onset of bronchoconstriction. Guinea-pigs were also injected intradermally with 2,4-dinitrochlorobenzene (DNCB), which is a potent skin sensitiser in man but which has not been reported to cause respiratory allergy. These animals developed only low-titre homocytotropic antibodies and were unresponsive to an inhalation challenge with either free or conjugated hapten. The animals were, however, contact-sensitised to the chemical.
Botham
P A
PA
ICI Central Toxicology Laboratory, Nr Macclesfield, Cheshire, U.K.
Rattray
N J
NJ
Woodcock
D R
DR
Walsh
S T
ST
Hext
P M
PM
D000280:Administration, Inhalation; D000818:Animals; D000917:Antibody Formation; D004137:Dinitrochlorobenzene; D000939:Epitopes; D005260:Female; D006168:Guinea Pigs; D006241:Haptens / Q000008:administration & dosage; D007114:Immunization; D007073:Immunoglobulin E / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D007271:Injections, Intradermal; D010795:Phthalic Acids / Q000633:toxicity*; D010796:Phthalic Anhydrides / Q000008:administration & dosage / Q000633:toxicity*; D012130:Respiratory Hypersensitivity / Q000139:chemically induced* / Q000276:immunology
D004137:Dinitrochlorobenzene; D000939:Epitopes; D006241:Haptens; D007074:Immunoglobulin G; D010795:Phthalic Acids; D010796:Phthalic Anhydrides; D007073:Immunoglobulin E; C015559:trimellitic anhydride