Demonstration of human, rye grass pollen extract-specific, helper and suppressor stimulating activity of modified allergens by effects on in vitro hapten-specific antibody production.
An in vivo system is described in which penicilloyl antibody was produced from peripheral leucocytes of a grass pollen-sensitive patient who had received penicillin therapy, by challenge of the cells with penicilloyl-grass pollen extract conjugate. Incubation of these leucocytes with a number of modified preparations of grass pollen extract with various T-cell-stimulating properties was shown to affect penicilloyl antibody production. Both chymotryptically fragmented rye grass pollen extract and a conjugate of f met-leu-phe and rye grass pollen extract enhanced penicilloyl-specific antibody similarly to the enhancement induced by unmodified extract, though at high concentration some suppression was seen. A conjugate of polysarcosine and rye grass pollen extract, previously shown to cause antibody suppression in mice, was similarly suppressive for penicilloyl-specific antibody. The system therefore shows potential for the evaluation of the effects of modified allergen treatment on antibody levels via T-cell mechanisms.
Wheeler
A W
AW
Institutionen för Klinisk Immunologi, Karolinska Sjukhuset, Stockholm, Sweden.
Johansson
S G
SG
D000485:Allergens / Q000276:immunology*; D000917:Antibody Formation; D004342:Drug Hypersensitivity / Q000276:immunology; D002523:Edible Grain / Q000276:immunology*; D006241:Haptens; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000276:immunology; D010406:Penicillins / Q000276:immunology; D010455:Peptides / Q000276:immunology; D011058:Pollen / Q000276:immunology*; D012521:Sarcosine / Q000031:analogs & derivatives / Q000276:immunology; D012434:Secale / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
D000485:Allergens; D006241:Haptens; D010406:Penicillins; D010455:Peptides; C015475:polysarcosine; D009240:N-Formylmethionine Leucyl-Phenylalanine; D012521:Sarcosine