HLA-DR beta III and HLA-DP induce comparable proliferation in primary mixed lymphocyte culture.
To study the stimulatory capacity of HLA-DR beta III in the mixed lymphocyte culture (MLC) assay, an MLC matrix between 13 Dw18 homozygous typing cells (HTCs) was analysed. Six of these HTCs were positive for the HLA-DR beta III allele LB-Q1 as defined by T cell clones. Seven HTCs were positive for LB-Q4. The MLC responses between Dw18 HTCs, matched for LB-Q, were significantly lower than the responses between the mismatched combinations. Considering the fact that HLA-DP can induce proliferation in MLC, we then analysed the DP matched and mismatched combinations separately. The influence of HLA-DR beta III mismatches was in our experiments comparable to the influence of mismatches for HLA-DP. Surprisingly, 15 out of 22 DR beta III and DP matched combinations still showed positive MLC reactions.
Schroeijers
W E
WE
Department of Immunohematology, Leiden University Hospital, The Netherlands.
de Koster
H S
HS
van Rood
J J
JJ
Termijtelen
A
A
D002478:Cells, Cultured; D000939:Epitopes; D006682:HLA-DP Antigens / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D006684:HLA-DR Antigens / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D059810:HLA-DR Serological Subtypes; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D007959:Lymphocyte Culture Test, Mixed; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology*
D000939:Epitopes; D006682:HLA-DP Antigens; D006684:HLA-DR Antigens; D059810:HLA-DR Serological Subtypes; C051104:HLA-DR52 antigen