Response of exocrine pancreas to corticosterone and aldosterone after adrenalectomy.
The long-term effect of adrenalectomy (Adx) on the exocrine pancreas was examined in female adult rats. Pancreatic amylase concentration decrease to 50% of the control level starting 10 days after Adx, whereas the levels of trypsinogen and lipase remained unchanged. Replacement studies beginning 24 h after surgery with corticosterone (B, 1 mg/100 g body wt) or aldosterone (ALDO, 8 micrograms/100 g body wt) alone did not prevent the decline in amylase after Adx. However, when both hormones were administered together, pancreatic amylase concentration was maintained at a level similar to that of the control group. Serum corticosterone levels in the rats receiving B alone or B + ALDO were not different, and were comparable to levels found in normal rats. Both ALDO and B, given for 5 days starting 10 days after Adx, were required to restore amylase concentrations toward control values. When spironolactone (SPIRO, 3 mg/100 g body wt), a specific mineralocorticoid receptor blocker was administered bid together with ALDO + B, it blocked the increase in pancreatic amylase seen in ALDO + B treated rats but did not affect the serum corticosterone levels. These results suggest that mineralocorticoids are also involved in modulating the level of amylase in the rat exocrine pancreas.
Alliet
P
P
Department of Pediatrics, Children's Hospital, SUNY, Buffalo 14222.
Lu
R B
RB
Madrazo de la Garza
J A
JA
Santer
R
R
Lebenthal
E
E
Lee
P C
PC
D000311:Adrenal Glands / Q000502:physiology; D000315:Adrenalectomy; D000450:Aldosterone / Q000502:physiology*; D000681:Amylases / Q000378:metabolism; D000818:Animals; D001835:Body Weight; D003345:Corticosterone / Q000502:physiology*; D005260:Female; D010179:Pancreas / Q000201:enzymology / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D013148:Spironolactone / Q000378:metabolism
D013148:Spironolactone; D000450:Aldosterone; D000681:Amylases; D003345:Corticosterone