Cloning and pretranslational hormonal regulation of testosterone 16 alpha-hydroxylase (P-45016 alpha) in male rat liver.
cDNA clones for P-45016 alpha were isolated from a male liver lambda gt11 expression library using antibodies against P-45016 alpha. The clones encompassed 1633 and 1791 bp, respectively. The latter clone contained the whole coding sequence. The 20 deduced NH2-terminal amino acids were identical to those of P-450h and the cDNA sequence was in complete agreement with that of P-450 (M-1). Northern blots showed that P-45016 alpha in the rat liver is pretranslationally regulated by the growth hormone secretory pattern. Southern blots indicated that few genes belong to the same P-450 gene family as P-45016 alpha.
Ström
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A
Department of Medical Nutrition, Karolinska Institute, Huddinge University Hospital, Sweden.
Mode
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Zaphiropoulos
P
P
Nilsson
A G
AG
Morgan
E
E
Gustafsson
J A
JA
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases; D001483:Base Sequence; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000235:genetics*; D005260:Female; D008099:Liver / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D039181:Steroid 16-alpha-Hydroxylase; D013250:Steroid Hydroxylases / Q000235:genetics* / Q000378:metabolism
D012313:RNA; D004247:DNA; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D001189:Aryl Hydrocarbon Hydroxylases; D039181:Steroid 16-alpha-Hydroxylase; C022724:testosterone 7-alpha-hydroxylase, hamster