Differential effects of calcium channel blockers and stimulants on morphine withdrawal in vitro.
The effects of calcium channel blockers and stimulants on naloxone-precipitated morphine withdrawal in morphine-dependent ileum were evaluated in vitro. Both verapamil and diltiazem (0.01-1 microM) inhibited the naloxone-induced morphine withdrawal contractures in a concentration-dependent way. The effect of diltiazem was stereo-specific. On the other hand, the calcium channel stimulant, Bay k 8644 (0.01 microM), significantly increased the naloxone-induced contractures of morphine-dependent ileum. These results suggest a role for calcium channels in morphine withdrawal in vitro.
Barrios
M
M
Department of Pharmacology, University of Granada Medical School, Spain.
Baeyens
J M
JM
D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / Q000494:pharmacology; D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D015220:Calcium Channels / Q000187:drug effects*; D004110:Diltiazem / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology; D005260:Female; D066298:In Vitro Techniques; D009020:Morphine / Q000009:adverse effects*; D009119:Muscle Contraction / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D009270:Naloxone / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013237:Stereoisomerism; D013375:Substance Withdrawal Syndrome / Q000503:physiopathology*; D014700:Verapamil / Q000494:pharmacology
D002121:Calcium Channel Blockers; D015220:Calcium Channels; D009270:Naloxone; D000431:Ethanol; D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; D009020:Morphine; D014700:Verapamil; D004110:Diltiazem