Recent advances in brain-gut hormones.
Although recent advances in gene technology have made it possible to produce peptides and proteins by the recombinant technique, chemical synthesis either by the solution or by the solid-phase technique is still only a method of choice for the syntheses of a brain-gut hormone and its superagonist and/or antagonist. In addition, synthetic replicates of brain-gut hormones, their analogus and fragments or their precursor-related peptides are important haptenic immunogens to produce region-specific antisera to the respective peptides. In the present study, antisera against proglucagon and pro-LH-RH related peptides were proved to be useful not only for identifying hormone-producing cells, but also for demonstrating the post-translational biosynthetic processing in the cells. For galanin, a novel brain-gut hormone, have structural similarity in its C-terminal region to tachykinins, a monoclonal antibody against synthetic porcine galanin (1-15) was prepared for immunohistochemical study. This monoclonal antibody was found to recognize specifically galanin-producing cells and not to cross-react with any of tachykinins examined. These observations indicate that monoclonal antibodies are highly useful for identifying cells possessing a brain-gut hormone containing structures common to those of different known peptides.
Yanaihara
N
N
Laboratory of Bioorganic Chemistry, University of Shizuoka School of Pharmaceutical Sciences, Japan.
Yanaihara
C
C
Zhang
T
T
Hoshino
M
M
Iguchi
K
K
Mochizuki
T
T
D000818:Animals; D019004:Galanin; D005934:Glucagon / Q000378:metabolism*; D004763:Glucagon-Like Peptides; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D010455:Peptides / Q000032:analysis / Q000378:metabolism*; D052336:Proglucagon; D011498:Protein Precursors / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D011995:Recombination, Genetic
D010455:Peptides; D011498:Protein Precursors; D011994:Recombinant Proteins; D007987:Gonadotropin-Releasing Hormone; D052336:Proglucagon; D004763:Glucagon-Like Peptides; C015236:glucagon-like-immunoreactivity; D019004:Galanin; D005934:Glucagon