Use of the polymerase chain reaction for the differential cross screening of libraries cloned into phage-lambda-based vectors.
We describe a simple and rapid method that can be used to identify sequences present in any two DNA libraries (either genomic or cDNA), provided only that the libraries are in different vectors with different cloning sites. This procedure makes use of the polymerase chain reaction (PCR) to amplify the inserts of one library. The product of the PCR reaction is then used to screen a second library to identify sequences which are common to both. We illustrate the use of this method for the systematic isolation of human X-chromosome-linked genomic clones that harbor sequences expressed in human chorioretinal tissue.
Wong
P
P
Department of Biochemistry, University of Ottawa, Ontario, Canada.
MacDonald
I
I
Tenniswood
M
M
D000818:Animals; D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D006224:Cricetinae; D015723:Gene Library; D005822:Genetic Vectors; D015698:Genomic Library; D006801:Humans; D006822:Hybrid Cells / Q000378:metabolism; D021141:Nucleic Acid Amplification Techniques; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA