An enzyme-linked immunosorbent assay for the determination of the IgA subclass distribution of antigen-specific antibodies.
An ELISA for the determination of the IgA subclass distribution of antigen-specific antibodies was developed using commercially available monoclonal anti-IgA1 anti-IgA2 subclass antibodies. Furthermore an anti-A2m allotype-specific antibody was included in the study. The specificity and sensitivity of the monoclonal anti-immunoglobulin antibodies used was analyzed using sera from normal and IgA class- or subclass-deficient individuals (with or without homozygous C alpha 1 subclass gene deletions). Human IgA1 and IgA2 hybridoma antibodies were also used. In this particular assay, only two out of four tested anti-IgA1 and two out of three tested anti-IgA2 antibodies proved to be specific for their corresponding IgA subclass. The anti-A2m(2) monoclonal antibody was shown to be specific for the corresponding allotype. These ELISA methods may facilitate further work on the regulation of IgA subclass production in man.
Engström
P E
PE
Department of Clinical Immunology, Karolinska Institute, Huddinge, Sweden.
Norhagen
G
G
Smith
C I
CI
Söder
P O
PO
Hammarström
L
L
D000361:Agammaglobulinemia / Q000235:genetics / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000145:classification*; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D002872:Chromosome Deletion; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis / Q000145:classification*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007070:Immunoglobulin A