Molecular characterization of rat skeletal muscle myosin light chain kinase.
A 1.85-kilobase (kb) cDNA has been isolated that encodes the catalytic and calmodulin binding domains of rat skeletal muscle myosin light chain kinase. The cDNA hybridized to a 3.3-kb RNA present in fast- and slow-twitch skeletal muscles. The reported enzymatic activity (3-fold greater in fast- than slow-twitch skeletal muscles) reflects the relative abundance of this RNA in the two types of skeletal muscle. No hybridization of the cDNA was detected to RNA isolated from smooth or nonmuscle tissues. The clone cross hybridized to a 2.2-kb RNA present in cardiac tissue. Ribonuclease protection analysis of skeletal and cardiac muscle RNA revealed major differences in the two hybridizing RNAs. Thus rat skeletal muscle contains a single myosin light chain kinase isoform, which is distinct from the cardiac, smooth, and nonmuscle forms.
Herring
B P
BP
Department of Physiology and Moss Heart Center, University of Texas, Southwestern Medical Center, Dallas 75235.
Nunnally
M H
MH
Gallagher
P J
PJ
Stull
J T
JT
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000378:metabolism; D005796:Genes; D008969:Molecular Sequence Data; D009132:Muscles / Q000201:enzymology*; D009219:Myosin-Light-Chain Kinase / Q000235:genetics*; D015345:Oligonucleotide Probes; D012313:RNA / Q000235:genetics; D016372:RNA, Antisense; D012333:RNA, Messenger / Q000037:antagonists & inhibitors; D051381:Rats
D004274:DNA, Recombinant; D015345:Oligonucleotide Probes; D016372:RNA, Antisense; D012333:RNA, Messenger; D012313:RNA; D009219:Myosin-Light-Chain Kinase