The heterogeneity of lipopolysaccharides from Citrobacter 023 serotype.
Chemical studies on carbohydrate part of lipopolysaccharides isolated from Citrobacter 87/57 and Citrobacter 1556 strains (serotype 023) were carried out. Lipopolysaccharides (LPS) were hydrolyzed with 1% acetic acid and the carbohydrate material was fractionated on Sephadex G-50 and Bio-Gel P-4 columns. All fractions obtained were subjected to chemical analysis by colorimetric methods and by gas-liquid chromatography. It was found that O-specific polysaccharide from LPS 87/57 is composed of mannose and N-acetylgalactosamine in the molar ratio of 3:1. Both lipopolysaccharides contain unsubstituted core oligosaccharides (built of heptose, glucose and galactose in molar ratio of 3:3:1) as well as core oligosaccharides substituted with shorter (LPS 87/57 and LPS 1556) and longer (LPS 87/57) O-specific oligosaccharides. Strain 87/57 is of S (smooth) type and strain 1556 lacking O-specific polysaccharide is of SR (semi-rough) type. The data of Sephadex G-100 chromatography showed that O-antigen from Citrobacter 87/57 is heterogeneous; it contains polysaccharide chains of different length but the same composition. The heterogeneity of Citrobacter 023 lipopolysaccharides has been also detected by SDS-polyacrylamide gel electrophoresis.
Katzenellenbogen
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Department of Immunochemistry, Polish Academy of Sciences, Wrocław.
D000116:Acetylgalactosamine / Q000032:analysis; D005591:Chemical Fractionation; D002849:Chromatography, Gas; D002954:Citrobacter / Q000032:analysis* / Q000276:immunology; D003124:Colorimetry; D004586:Electrophoresis; D000939:Epitopes / Q000032:analysis; D008070:Lipopolysaccharides / Q000032:analysis*; D008358:Mannose / Q000032:analysis; D012703:Serotyping
D000939:Epitopes; D008070:Lipopolysaccharides; D000116:Acetylgalactosamine; D008358:Mannose