Mechanism of decreased insulinogenesis in manganese-deficient rats. Decreased insulin mRNA levels.
Manganese-deficient rats exhibited seven-fold lower preproinsulin mRNA levels compared to control, as detected by dot blot hybridization of both total and poly(A)+ RNA using a preproinsulin cDNA probe. No differences in the size of the insulin mRNA were observed. Thus, decreased mRNA levels may be a major contributing factor to the decreased insulinogenesis observed in manganese-deficient rats.
Baly
D L
DL
Department of Nutrition, Rutgers University, New Brunswick, NJ 08903.
Lee
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Doshi
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D000818:Animals; D015152:Blotting, Northern; D005260:Female; D007328:Insulin; D007515:Islets of Langerhans / Q000378:metabolism*; D008297:Male; D008345:Manganese / Q000172:deficiency*; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000235:genetics; D011384:Proinsulin / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics*; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values
D007328:Insulin; D011498:Protein Precursors; D012333:RNA, Messenger; D011061:Poly A; D008345:Manganese; C013737:preproinsulin; D012313:RNA; D011384:Proinsulin