Calcium metabolism and bombesin-stimulated pancreatic enzyme secretion.
In dispersed guinea pig pancreatic acini, bombesin stimulates a rapid increase in the concentration of free cytosolic Ca2+ ([Ca2+]i) by releasing Ca2+ from an intracellular agonist-sensitive pool. This increase is transient, and it mediates the initial secretion of digestive enzymes from the acinar cell. During sustained bombesin stimulation, [Ca2+]i returns to the resting or near-resting level, but bombesin-stimulated enzyme secretion continues. Although [Ca2+]i is at the resting level during continued stimulation, 45Ca2+ flux measurements indicate that bombesin continues to release Ca2+ from an intracellular store that is probably the agonist-sensitive pool. Low concentrations (0.3 microM) of the non-fluorescent Ca2+ ionophore, 4-bromo-A23187 (Br-A23187), release Ca2+ from the agonist-sensitive pool without measurably increasing [Ca2+]i. This ionophore-induced Ca2+ release without production of an increase in [Ca2+]i does not by itself stimulate enzyme secretion, but it does augment the secretion caused by the phorbol ester, 4 beta-phorbol, 12-myristate, 13-acetate (PMA). These results suggest a role for intracellular Ca2+ release in bombesin-stimulated sustained secretion.
Pandol
S J
SJ
Department of Medicine, Veterans Administration Medical Center, San Diego, California.
Mendius
K E
KE
D000681:Amylases / Q000378:metabolism*; D000818:Animals; D001572:Benzofurans; D001839:Bombesin / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D016257:Fura-2; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007700:Kinetics; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D001572:Benzofurans; D000001:Calcimycin; D000681:Amylases; D013755:Tetradecanoylphorbol Acetate; D001839:Bombesin; D002118:Calcium; D016257:Fura-2