A method for staining and stabilizing peroxidase activity in polyacrylamide gel electrophoresis.
A procedure was developed for a rapid double staining of peroxidase and other proteins in the same polyacrylamide gels using guaiacol and Coomassie blue. The distinguishable colored bands of peroxidase isozymes and proteins are stable for at least 8 months.
Shimoni
M
M
Division of Plant Pathology, Agricultural Research Organization, Newe-Ya'ar, Israel.
Reuveni
R
R
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D004795:Enzyme Stability; D006139:Guaiacol; D007527:Isoenzymes / Q000302:isolation & purification; D010544:Peroxidases / Q000302:isolation & purification*; D010944:Plants / Q000201:enzymology; D012394:Rosaniline Dyes; D013194:Staining and Labeling / Q000379:methods*
D007527:Isoenzymes; D012394:Rosaniline Dyes; C024757:Coomassie brilliant blue R; D006139:Guaiacol; D010544:Peroxidases