Sequential estimation of nuclear DNA and silver staining of nucleoli in plant cells.
A method for sequential estimation of nuclear DNA and silver staining of nucleoli in plant cells is described. Feulgen staining is done first and nuclear DNA estimated by absorption cytophotometry. Following this, the slides are stained with AgNO3. The method has been used to study the process of nucleolar fusion in garlic (Allium sativum L.) meristem root tip cells. It was found that during interphase nucleoli rarely fused, thus most fusion must have occurred before the G1 phase of the cell cycle.
Dolezel
J
J
Institute of Experimental Botany, Department of Plant Biotechnology, Olomouc, Czechoslovakia.
Cíhalíková
J
J
Zakchlenjuk
O V
OV
D004396:Coloring Agents; D004247:DNA / Q000032:analysis*; D009697:Nucleolus Organizer Region / Q000032:analysis*; D059828:Plant Cells; D010944:Plants / Q000032:analysis*; D012394:Rosaniline Dyes; D012835:Silver Nitrate; D013194:Staining and Labeling
D004396:Coloring Agents; C007722:Feulgen stain; D012394:Rosaniline Dyes; D004247:DNA; D012835:Silver Nitrate