Deoxyribonucleic acid (DNA) damage induced by bleomycin-Fe(II) in vitro: formation of 8-hydroxyguanine residues in DNA.
Treatment of calf thymus deoxyribonucleic acid (DNA) with bleomycin-Fe(II) at 0 degree C for 5 min resulted in the formation of 8-hydroxyguanine (8-OH-Gua) residues in DNA in a dose dependent manner, in addition to the formation of base propenal, a DNA-degradation product. The amount of 8-OH-Gua was about one-hundredth of that of base propenal. Treatment of cellular DNA with bleomycin did not result in any increase of 8-OH-Gua even under conditions of 100% cell killing.
Kohda
K
K
Kasai
H
H
Ogawa
T
T
Suzuki
T
T
Kawazoe
Y
Y
D001761:Bleomycin / Q000494:pharmacology*; D002478:Cells, Cultured; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000032:analysis / Q000187:drug effects*; D006147:Guanine / Q000031:analogs & derivatives* / Q000032:analysis
D004273:DNA, Neoplasm; D001761:Bleomycin; C024829:8-hydroxyguanine; D006147:Guanine