title: The time course of liver DNA and RNA alterations in acute experimental pancreatitis in rats--a possible mechanism of prostacyclin (PGI2) protection.
abstract: The mechanism of the liver damage in acute pancreatitis remains unsolved. The aim of this study was to evaluate RNA and DNA changes in the liver in Na-taurocholate pancreatitis in rats, with consideration being given to the protective effect of prostacyclin (20 micrograms.kg.24 h intraperitoneally). Total RNA increased both in treated and untreated rats after 24 hrs and even more after 48 hrs. After 48 hrs this increase was 35% higher in treated than in untreated rats. The RNA/DNA ratio paralleled the increase of total RNA. In 24 hrs incorporation of 3H-uridine into RNA increased 145% in untreated, and in 48 hrs 50% in treated rats. After 7 days 60% of treated animals survived as compared with 20% in the untreated group. The evident changes in RNA content, RNA/DNA ratio and RNA synthesis suggest a role for nucleic acid disturbances in the liver injury in acute pancreatitis. The beneficial effect of prostacyclin might, in part, be dependent on its effect on nucleic acid metabolism.
authors: Gastroenterology Department, Medical Academy, Bialystok, Poland.
authors: G
authors: G
authors: Jurkowska
authors: J
authors: J
authors: Dlugosz
authors: A
authors: A
authors: Gabryelewicz
authors: A
authors: A
authors: Andrzejewska
mesh_terms: D000208:Acute Disease; D000818:Animals; D004247:DNA / Q000378:metabolism*; D011464:Epoprostenol / Q000494:pharmacology* / Q000627:therapeutic use; D008099:Liver / Q000378:metabolism*; D008297:Male; D010195:Pancreatitis / Q000188:drug therapy / Q000378:metabolism* / Q000517:prevention & control; D012313:RNA / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
chemical_list: D012313:RNA; D004247:DNA; D011464:Epoprostenol