Determination of the primary structure of PLC-154 demonstrates diversity of phosphoinositide-specific phospholipase C activities.
Protein sequence analysis of a bovine brain phosphoinositide-specific phospholipase C (PI-PLC; PLC-154) has permitted the isolation of a cDNA that appears to code for this protein. Transient expression of this cDNA in COS-1 cells demonstrates that the cDNA encodes a functional phospholipase C that migrates at approximately 150,000 daltons. A transcript of approximately 7 kb is observed in RNA derived from bovine brain and a related transcript of the same size is present in certain human cell lines. Southern blot analysis indicates that one or possibly two genes hybridize with a PLC-154 probe. Regions of homology between PLC-154 and the previously described PLC-148 allow the assignment of a putative catalytic domain to the central region of PLC-154.
Katan
M
M
Ludwig Institute for Cancer Research, London, England.
Kriz
R W
RW
Totty
N
N
Philp
R
R
Meldrum
E
E
Aldape
R A
RA
Knopf
J L
JL
Parker
P J
PJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010716:Phosphatidylinositols / Q000378:metabolism*; D012313:RNA / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013379:Substrate Specificity; D014158:Transcription, Genetic; D010738:Type C Phospholipases / Q000235:genetics* / Q000378:metabolism
D010716:Phosphatidylinositols; D012313:RNA; D004247:DNA; D010738:Type C Phospholipases