RNA and protein synthesis requirements for the resumption of meiosis in rabbit oocytes: the role of cumulus cells.
In vitro maturation of rabbit cumulus-enclosed oocytes was fully inhibited in alpha-amanitin- (100 micrograms/ml) and cycloheximide- (5 micrograms/ml) supplemented media. The inhibition was reversible and substantially reduced by delaying the addition of alpha-amanitin (2h) or cycloheximide (3 h). In contrast, both drugs did not inhibit germinal vesicle breakdown in denuded oocytes. Co-culture of granulosa cells (1 x 10(6)/ml) with denuded oocytes did not substitute for an intact cumulus. The data presented here suggest that the resumption of meiosis in rabbit cumulus-enclosed oocytes is dependent upon early transcriptional and translational events which probably occur within the cumulus cells.
Motlík
J
J
Czechoslovak Academy of Sciences, Institute of Animal Physiology and Genetics, Department of Genetics, Libĕchov.
Fulka
J
J
Procházka
R
R
Rimkevicová
Z
Z
Kubelka
M
M
Fulka
J
J
D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D005260:Female; D008540:Meiosis / Q000187:drug effects; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006080:Ovarian Follicle / Q000166:cytology* / Q000502:physiology; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*; D011817:Rabbits; D014158:Transcription, Genetic
D000546:Amanitins; D012313:RNA; D003513:Cycloheximide