Retention of differentiated characteristics in human fetal keratinocytes in vitro.
Many of the morphologic and biochemical changes that occur during human fetal skin development have been described, yet there has been little experimental analysis of the processes that regulate the development of human fetal skin. This is due in part to difficulties in culturing human fetal epidermal keratinocytes. We have successfully cultured fetal keratinocytes in two different in vitro systems; in a serum-free keratinocyte growth medium (KGM) on tissue culture plastic and cocultured with dermal fibroblasts as spheroidal aggregates. To characterize these fetal keratinocytes in vitro we have assessed their ability to express several markers of epidermal differentiation. Human fetal keratinocytes grown on plastic in KGM stratify and express some of the components of the differentiated epidermis, such as involucrin and the high molecular weight keratins. However, these keratinocytes co-express keratins and vimentin and do not form a structured basement membrane. More characteristics of fetal skin are preserved in mixed aggregates of epidermal keratinocytes and dermal fibroblasts, including epidermal stratification, synthesis of basement membrane components, tissue-specific expression of intermediate filaments, involucrin, and expression of high molecular weight keratins. The maintenance of human fetal epidermal keratinocytes in these two in vitro systems and their ability to express many differentiated characteristics suggests that these cultures will be valuable for studies of the molecular mechanisms that regulate the regionally specific differentiation of the human fetal epidermis.
Haake
A R
AR
Department of Dermatology, University of Rochester Medical Center, New York 14642.
Lane
A T
AT
D000911:Antibodies, Monoclonal; D001485:Basement Membrane; D002449:Cell Aggregation; D002454:Cell Differentiation; D002478:Cells, Cultured; D003094:Collagen / Q000032:analysis; D003470:Culture Media; D000078404:Epidermal Cells; D004817:Epidermis / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D006801:Humans; D015151:Immunoblotting; D007633:Keratins / Q000032:analysis / Q000378:metabolism*; D007797:Laminin / Q000032:analysis; D008970:Molecular Weight; D011498:Protein Precursors / Q000032:analysis; D012867:Skin / Q000196:embryology*; D014746:Vimentin / Q000032:analysis
D000911:Antibodies, Monoclonal; D003470:Culture Media; D007797:Laminin; D011498:Protein Precursors; D014746:Vimentin; C032253:involucrin; D007633:Keratins; D003094:Collagen