[The role of tryptophan residues in the antigenic activity of carcinoembryonic antigen].
Antigenic determinants of carcino-embryonic antigen (CEA) were spatially located using N-bromosuccinimide modification of tryptophan residues both in native (acetate buffer solution) and unfolded (guanidinium chloride solution) molecule of the antigen. Modification of exposed tryptophan residues failed to alter CEA antigenic activity and conformation of its protein portion as shown by CD spectroscopy. On the contrary, modification of buried tryptophan residues induced conformational changes of CEA protein portion connected with a considerable loss of its antigenic activity. It was shown that CEA antigenic activity depends on spatial structure of its protein moiety.
Glazunov
V P
VP
Vakorina
T I
TI
Odinokov
S E
SE
Kurika
A V
AV
Pavlenko
A F
AF
D001974:Bromosuccinimide; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D002942:Circular Dichroism; D000939:Epitopes / Q000032:analysis*; D006801:Humans; D007202:Indicators and Reagents; D011487:Protein Conformation; D014364:Tryptophan / Q000032:analysis*
D002272:Carcinoembryonic Antigen; D000939:Epitopes; D007202:Indicators and Reagents; D014364:Tryptophan; D001974:Bromosuccinimide
Rol' ostatkov triptofana v antigennoĭ aktivnosti rakovo-émbrional'nogo antigena.