In vitro immunologic studies on a population of workers exposed to phthalic and tetrachlorophthalic anhydride.
In vitro radioallergosorbent tests have not been useful in identification of subjects with symptomatic allergic responses to acid anhydrides. By using phthalic anhydride or tetrachlorophthalic anhydride conjugated to human serum albumin, a study was undertaken to determine whether histamine release from basophils or lymphocyte transformation correlated with clinical symptoms, circulating anhydride specific IgE, and skin test reactivity. The data demonstrate that only histamine release from basophils correlated with symptoms and skin test reactivity. We conclude that in vitro histamine assays can be used in the identification of subjects with allergic responses to anhydrides.
Flaherty
D K
DK
Environmental Health Laboratory, Monsanto Agricultural Company, St Louis, MO 63110.
Gross
C J
CJ
Winzenburger
P
P
Compas
M B
MB
McGarity
K
K
Tillman
E
E
D000328:Adult; D001491:Basophils / Q000187:drug effects; D005260:Female; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D010795:Phthalic Acids / Q000494:pharmacology*; D010796:Phthalic Anhydrides / Q000494:pharmacology*; D012882:Skin Tests
D010795:Phthalic Acids; D010796:Phthalic Anhydrides; D007073:Immunoglobulin E; C009409:tetrachlorophthalic anhydride; C043103:phthalic anhydride