The sulfated polysaccharide-peptidoglycan complex potently inhibits embryonic angiogenesis and tumor growth in the presence of cortisone acetate.
In combination with cortisone acteate, the sulfated polysaccharide (SP)-peptidoglycan (PG) complex produced by an Arthrobacter species was found to inhibit both embryonic angiogenesis of chick chorioallantoic membrane (CAM) and growth of solid Sarcoma 180 tumor in mice. Three fractions obtained from the SP-PG complex by gel filtration showed a great difference in the inhibitory effect on angiogenesis of the CAM, whereas in ex vivo study such a difference was reduced. Of the three fractions, SP-PG-L having the lowest molecular weight and peptidoglycan content exhibited the highest antiangiogenic and antitumor activities, even higher than those of heparin. Neither desulfation nor sulfation of the SP-PG complex greatly affected the antiangiogenic activity, and dextran sulfates and mannoglucan sulfates were lacking the activity. This suggested that glycosyl sequences of sulfated polysaccharides are more important for the activity, rather than content or intramolecular distribution of sulfate groups.
Inoue
K
K
Research Institute of Daiichi Seiyaku Co., Ltd., Tokyo, Japan.
Korenaga
H
H
Tanaka
N G
NG
Sakamoto
N
N
Kadoya
S
S
D000482:Allantois / Q000098:blood supply / Q000187:drug effects; D000818:Animals; D002642:Chick Embryo; D002823:Chorion / Q000098:blood supply / Q000187:drug effects; D003348:Cortisone / Q000008:administration & dosage / Q000031:analogs & derivatives; D004357:Drug Synergism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009389:Neovascularization, Pathologic / Q000517:prevention & control*; D010457:Peptidoglycan / Q000008:administration & dosage / Q000494:pharmacology*; D011134:Polysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D012510:Sarcoma 180 / Q000188:drug therapy*
D010457:Peptidoglycan; D011134:Polysaccharides; D003348:Cortisone