Inhibitory effect of flavonoids on DNA-dependent DNA and RNA polymerases.
Flavonoids, (-)-epigallocatechin (1), myricetin (2) and quercetin (3), were investigated for inhibitory effects on E. coli DNA polymerase I and T7 bacteriophage RNA polymerase. In both DNA and RNA synthesis, 1 and 3 inhibited enzyme reactions by non-competitive and mixed type inhibition respectively, with regard to template DNAs. Myricetin (2) inhibited DNA and RNA polymerase reactions by mixed type and competitive type inhibition, respectively, with template DNAs. It was suggested that 2 interacts with covalently closed circular DNA.
Shinozuka
K
K
Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Kikuchi
Y
Y
Nishino
C
C
Mori
A
A
Tawata
S
S
D000818:Animals; D002392:Catechin / Q000031:analogs & derivatives*; D002417:Cattle; D004247:DNA / Q000096:biosynthesis; D004256:DNA Polymerase I / Q000037:antagonists & inhibitors*; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors*; D004926:Escherichia coli / Q000201:enzymology; D005419:Flavonoids / Q000494:pharmacology*; D011794:Quercetin / Q000494:pharmacology*; D012313:RNA / Q000096:biosynthesis; D013604:T-Phages / Q000201:enzymology; D013698:Templates, Genetic
D005419:Flavonoids; D012313:RNA; C040015:myricetin; D002392:Catechin; D004247:DNA; D011794:Quercetin; D012321:DNA-Directed RNA Polymerases; D004256:DNA Polymerase I; C057580:gallocatechol