Interactions of iloprost and sodium nitroprusside on vascular smooth muscle and platelet aggregation.
1. The aim of the study was to assess and quantify any synergism occurring between the stable analogues of prostacyclin (iloprost) and nitric oxide (sodium nitroprusside) with respect to both relaxation of vascular smooth muscle and inhibition of platelet aggregation in the rabbit. 2. Iloprost (0.3-3 ng ml-1) and sodium nitroprusside (0.3-3 ng ml-1) caused dose-dependent relaxation of the rabbit mesenteric and coeliac arteries. 3. Iloprost (0.3-30 ng ml-1) and sodium nitroprusside (0.03-30 micrograms ml-1) caused dose-dependent inhibition of rabbit platelet aggregation induced by adenosine diphosphate or collagen. 4. In combination, iloprost and sodium nitroprusside caused an inhibition of platelet aggregation that was 2-3 fold greater than would be expected by summation, while no such potentiation was observed on vascular smooth muscle. 5. Thus, our results indicate that under physiological conditions the mediators prostacyclin and endothelium-derived relaxing factor (NO) can exert a synergistic action on platelets, but have only an additive effect on vascular smooth muscle.
Lidbury
P S
PS
William Harvey Research Institute, St Bartholomew's Hospital Medical College, London.
Antunes
E
E
de Nucci
G
G
Vane
J R
JR
D000818:Animals; D002317:Cardiovascular Agents / Q000494:pharmacology*; D002445:Celiac Artery / Q000187:drug effects; D003094:Collagen / Q000494:pharmacology; D004347:Drug Interactions; D011464:Epoprostenol / Q000494:pharmacology*; D005292:Ferricyanides / Q000494:pharmacology*; D016285:Iloprost; D066298:In Vitro Techniques; D008638:Mesenteric Arteries / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D009599:Nitroprusside / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011817:Rabbits
D002317:Cardiovascular Agents; D005292:Ferricyanides; D010975:Platelet Aggregation Inhibitors; D009599:Nitroprusside; D003094:Collagen; D011464:Epoprostenol; D016285:Iloprost