Practical aspects of 2D NMR for assigning the non-exchangeable protons in DNA-RNA fragments.
Using the branched trimer A2'-5'A3'-5'A as an example, different 2D NMR experiments such as homonuclear correlations via single-quantum coherence, double-quantum filtration via double-quantum coherence, isotropic mixing, relayed connectivities, cross relaxation and 31P/1H correlations are presented together with the corresponding spectra. The discussion pointed out the advantages and the difficulties of 14 pulse sequences, used for assigning non-exchangeable protons in DNA or RNA fragments. The emphasis has been put on the methodological aspects of 2D NMR as a simple technique, allowing an easier assimilation of the new coming 2D NMR pulse sequences.
Remaud
G
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Department of Bioorganic Chemistry, University of Uppsala, Sweden.
D000227:Adenine Nucleotides; D004247:DNA; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D009690:Nucleic Acid Conformation; D010758:Phosphorus; D011131:Polyribonucleotides; D011522:Protons; D011789:Quantum Theory; D012313:RNA
D000227:Adenine Nucleotides; D011131:Polyribonucleotides; D011522:Protons; D010758:Phosphorus; D012313:RNA; D006859:Hydrogen; D004247:DNA