Characterization of nicotinic acetylcholine receptor channels of the TE671 human medulloblastoma clonal line.
Acetylcholine (ACh)-gated single channel events were studied on the TE671 human medulloblastoma clonal cell line by the use of the cell-attached patch clamp technique. Channel activity was detected (86% probability) in the presence of 0.1-2 microM ACh but not (0% probability) in the absence of agonist or in the presence of 1 microM alpha-bungarotoxin (Bgt). This effect of Bgt was reversible within 1 h. The most prominent channel type had a conductance of 50 pS. The kinetics of opening and closing of the channel were similar to that for skeletal muscle nicotinic acetylcholine receptors.
Oswald
R E
RE
Department of Pharmacology, College of Veterinary Medicine, Cornell University, Ithaca, NY.
Papke
R L
RL
Lukas
R J
RJ
D000109:Acetylcholine / Q000494:pharmacology; D002038:Bungarotoxins / Q000494:pharmacology; D006801:Humans; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology; D008527:Medulloblastoma; D011978:Receptors, Nicotinic / Q000187:drug effects / Q000502:physiology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*
D002038:Bungarotoxins; D007473:Ion Channels; D011978:Receptors, Nicotinic; D000109:Acetylcholine