Labile protecting groups in tRNA synthesis.
The use of labile protecting groups for the protection of the exocyclic amino function of adenine, guanine and cytosine has two main advantages in RNA synthesis. Final deprotection in concentrated aqueous ammonia takes place in milder conditions which are more compatible with the sensitivity of oligoribonucleotides towards alkali-conditions. The introduction of fragile bases such as certain modified bases encountered in the primary structure of t-RNA is feasible. The chemical synthesis of RNA fragments constituting the primary structure of B. subtilis f-methionine t-RNA is described.
Chaix
C
C
Département de Recherche Fondamentale, Centre d'Etudes Nucléaires de Grenoble, France.
Duplaa
A M
AM
Gasparutto
D
D
Molko
D
D
Téoule
R
R
D001412:Bacillus subtilis / Q000235:genetics; D001483:Base Sequence; D008969:Molecular Sequence Data; D011560:Pseudouridine; D012329:RNA, Bacterial / Q000138:chemical synthesis; D012345:RNA, Transfer, Amino Acid-Specific / Q000138:chemical synthesis*; D012358:RNA, Transfer, Met / Q000138:chemical synthesis*; D014529:Uridine / Q000031:analogs & derivatives
D012329:RNA, Bacterial; D012345:RNA, Transfer, Amino Acid-Specific; D012358:RNA, Transfer, Met; D011560:Pseudouridine; C010922:5,6-dihydrouridine; D014529:Uridine