Inhibition of human T-cell leukemia virus type I replication in primary human T cells that express antisense RNA.
The human T-cell leukemia virus type I is associated with adult T-cell leukemia-lymphoma in humans, a disease which is induced by a malignant transformation of T lymphocytes. Retrovirus vectors carrying human T-cell leukemia virus type I-derived sequences in reversed transcriptional orientation were used to express antisense RNA transcripts in primary human leukocytes. Human T-cell leukemia virus type I replication and virus-mediated immortalization were inhibited in cells harboring antisense constructs. This study suggests that retrovirus-mediated antisense RNA inhibition can be used to protect primary human T-lymphocytes from human T-cell leukemia virus type I-mediated cell transformation.
von Rüden
T
T
Institute of Genetics, Johannes Gutenberg University of Mainz, Federal Republic of Germany.
Gilboa
E
E
D002455:Cell Division; D002460:Cell Line; D004261:DNA Replication; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D015368:Human T-lymphotropic virus 1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D012313:RNA / Q000235:genetics*; D016372:RNA, Antisense; D013601:T-Lymphocytes / Q000382:microbiology*; D014779:Virus Replication
D016372:RNA, Antisense; D012313:RNA