[Use of solid-phase Clq-ELISA for detecting circulating immune complexes in human diseases].
The authors provide the results of studying the methodologic characteristics and of the clinical trial of the C1q-ELISA for detection of the circulating immune complexes. The C1q-ELISA is shown to have a high sensitivity and to make it possible to identify 3 micrograms/ml and more of aggregated gamma-globulin. The method can use for detection of CIC E (ab')2-fragments of antibodies to IgG of man and protein A labeled with peroxidase. Using the method, the circulating immune complexes were identified in the sera of 50% of patients with systemic lupus erythematosus, of 30% of patients with dilated cardiomyopathy and of 53% of patients with nonspecific aortoarteritis. It is concluded that the C1q-ELISA can be used in clinical practice for detecting the circulating immune complexes in different diseases of man.
Aleshkin
V A
VA
Nasonov
E L
EL
Eremeeva
M V
MV
Kobylianskiĭ
A G
AG
D000936:Antigen-Antibody Complex / Q000032:analysis*; D002311:Cardiomyopathy, Dilated / Q000276:immunology; D015922:Complement C1q / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005169:Factor VIII / Q000032:analysis; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D013625:Takayasu Arteritis / Q000276:immunology; D005719:gamma-Globulins / Q000032:analysis
D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D005719:gamma-Globulins; D015922:Complement C1q; D005169:Factor VIII
Ispol'zovanie tverdofaznogo Clq-immunofermentnogo metoda dlia opredeleniia tsirkuliruiushchikh immunnykh komplksov pri zabolevaniiakh cheloveka.