Temporal and constitutive expression of homeobox-2 gene (Hu-2), human heat shock gene (hsp-70), and oncogenes C-sis and N-myc in early human trophoblast.
Recent studies of the genetic basis of animal development indicate that homeobox genes, protooncogenes, and some heat shock genes may play a role in early embryogenesis. To investigate the possibility that these genes function in early human embryonic development, we monitored the expression of a human homeobox gene (Hu-2), two human protooncogenes (C-sis and N-myc), and a human heat shock gene (hsp-70) in human trophoblasts at 7 to 13 weeks gestational age. All these genes were found to be expressed in the tissues analyzed. The hsp-70 gene was expressed at nearly constant levels throughout the development period surveyed, whereas N-myc, C-sis, and Hu-2 showed a coordinated pattern of regulated expression. These results are consistent with a functional role of these genes in the early course of human development.
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Department of Obstetrics and Gynecology, Medical College of Georgia, Augusta 30912.
Strand
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D
McDonough
P G
PG
McDonald
J F
JF
D015152:Blotting, Northern; D004247:DNA; D005786:Gene Expression Regulation; D005801:Genes, Homeobox; D005865:Gestational Age; D006360:Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D009693:Nucleic Acid Hybridization; D009857:Oncogenes; D012313:RNA; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014327:Trophoblasts / Q000502:physiology*
D006360:Heat-Shock Proteins; D012313:RNA; D004247:DNA