Preparation and application of polyclonal antibodies to recombinant human interleukin-1 alpha and interleukin-1 beta.
We report the preparation and application of polyclonal antisera to the analysis and quantitation of human interleukin-1 alpha (IL-1 alpha) and human interleukin-1 beta (IL-1 beta). The anti IL-1 alpha antibodies specifically react with the alpha form of IL-1 and do not cross react (less than 0.1%) with the beta form of IL-1 and vice versa. Data reported here demonstrate that detection of human IL-1 alpha or beta by a radioimmunoassay technique is sensitive enough to measure picogram levels of these lymphokines. The practical application of using these highly specific antisera for radioimmunoassays was established by measuring exogenously added IL-1 alpha or IL-1 beta to human plasma. Potential benefits of these reagents and the radioimmunoassay procedures described herein are discussed in relation to the biological assays which cannot distinguish between human IL-1 alpha and human IL-1 beta.
Cornette
J C
JC
Upjohn Company, Kalamazoo, MI 49001.
Sharma
S K
SK
D000906:Antibodies / Q000302:isolation & purification*; D000918:Antibody Specificity; D003429:Cross Reactions; D000939:Epitopes; D014644:Genetic Variation; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D011863:Radioimmunoassay
D000906:Antibodies; D000939:Epitopes; D007375:Interleukin-1