Isolation and sequence of lambda gt11 cDNA clones encoding the 5B4 antigen expressed on sprouting neurons.
Monoclonal antibody (mAb) 5B4 recognizes a developmentally regulated membrane glycoprotein (Mr approximately 185,000-255,000) expressed on sprouting neurons. The amino acid sequence deduced from lambda gt11 cDNA clones encoding the transmembrane and cytoplasmic domains of the 5B4 antigen is co-linear with that of chick NCAM-ld. The significant level of overall sequence identity (75%) demonstrates that the 5B4 antigen is rat brain NCAM. The 5B4 epitope maps to the carboxy-terminus common to both NCAM-ld and NCAM-sd.
Ramos
P
P
Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas 75235-9050.
Safaei
R
R
Kayalar
C
C
Ellis
L
L
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000032:analysis*; D001483:Base Sequence; D015815:Cell Adhesion Molecules; D003712:Dendrites / Q000378:metabolism*; D000939:Epitopes; D008562:Membrane Glycoproteins / Q000032:analysis*; D008969:Molecular Sequence Data; D008970:Molecular Weight
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D000939:Epitopes; D008562:Membrane Glycoproteins