Double-staining methods at the ultrastructural level applying colloidal gold conjugates.
Several double gold-labeling methods have been reviewed, and our own results of applying some of these methods have been included. Of the six double-staining methods tested, four gave satisfactory results (indirect double immunogold staining method, two-face protein A-gold staining method, two-face amplified protein A-gold staining method, and formaldehyde blockade protein A-gold staining method). The other two techniques (sequential double protein A-gold staining method and formaldehyde blockade amplified protein A-gold staining method) showed cross-reaction between the first and second staining sequence.
Holm
R
R
Department of Pathology, Norwegian Radium Hospital, Oslo.
Nesland
J M
JM
Attramadal
A
A
Johannessen
J V
JV
D000236:Adenoma / Q000378:metabolism / Q000648:ultrastructure; D000936:Antigen-Antibody Complex / Q000276:immunology; D001426:Bacterial Proteins; D003429:Cross Reactions; D005557:Formaldehyde / Q000494:pharmacology; D013006:Growth Hormone / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D010911:Pituitary Neoplasms / Q000378:metabolism / Q000648:ultrastructure; D011388:Prolactin / Q000276:immunology; D013194:Staining and Labeling / Q000379:methods; D013205:Staphylococcal Protein A; D019809:Streptavidin
D000936:Antigen-Antibody Complex; D001426:Bacterial Proteins; D013205:Staphylococcal Protein A; D005557:Formaldehyde; D011388:Prolactin; D013006:Growth Hormone; D019809:Streptavidin