title: Immortalization of murine connective tissue-type mast cells at multiple stages of their differentiation by coculture of splenocytes with fibroblasts that produce Kirsten sarcoma virus.
abstract: Mature connective tissue mast cells (CTMC) have not been previously available as a cell line from any species. Here we describe 15 novel mast cell lines (KiSV-MC) that were derived by coculturing murine splenocytes with fibroblasts that produce a Ki-ras-containing murine sarcoma virus. Some of the KiSV-MC lines are similar to CTMC in that they synthesize predominantly heparin proteoglycans, and contain up to 35 micrograms of histamine and 2.2 units of carboxypeptidase A/10(6) cells in secretory granules which stain red with Safranin. Other cell lines display phenotypic characteristics intermediate to CTMC and mucosal-like mast cells in being predominantly Safranin-, having lower amounts of histamine and carboxypeptidase A, and in synthesizing chondroitin sulfate E proteoglycans in preference to heparin proteoglycans. When the individual KiSV-MC lines were compared, a linear relationship was found between the number of Safranin+ granules, the cellular contents of histamine and carboxypeptidase A, and the biosynthesis of heparin relative to chondroitin sulfate E proteoglycans. Upon sensitization with monoclonal IgE and exposure to hapten-specific antigen, the cells exocytose the contents of their secretory granules. Thus, these immortalized cells provide the first source of CTMC-like lines for chemical and functional analysis and illustrate that murine mast cells can express a continuum of phenotypes.
authors: Department of Medicine, Harvard Medical School, Boston, Massachusetts.
authors: D S
authors: DS
authors: Reynolds
authors: W E
authors: WE
authors: Serafin
authors: D V
authors: DV
authors: Faller
authors: D A
authors: DA
authors: Wall
authors: A K
authors: AK
authors: Abbas
authors: A M
authors: AM
authors: Dvorak
authors: K F
authors: KF
authors: Austen
authors: R L
authors: RL
authors: Stevens
mesh_terms: D000818:Animals; D002268:Carboxypeptidases / Q000378:metabolism; D043422:Carboxypeptidases A; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D011508:Chondroitin Sulfate Proteoglycans / Q000096:biosynthesis; D003239:Connective Tissue Cells; D003594:Cytoplasmic Granules / Q000378:metabolism; D005089:Exocytosis; D005347:Fibroblasts / Q000382:microbiology* / Q000502:physiology; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate / Q000096:biosynthesis; D006632:Histamine / Q000378:metabolism; D006651:Histocytochemistry; D007073:Immunoglobulin E / Q000276:immunology; D007708:Kirsten murine sarcoma virus / Q000502:physiology*; D008407:Mast Cells / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D008854:Microscopy, Electron; D010619:Phenazines; D010641:Phenotype; D009053:Sarcoma Viruses, Murine / Q000502:physiology*; D013154:Spleen / Q000166:cytology / Q000502:physiology*; D013194:Staining and Labeling; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
chemical_list: D002268:Carboxypeptidases; D043422:Carboxypeptidases A; D011508:Chondroitin Sulfate Proteoglycans; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D006632:Histamine; D007073:Immunoglobulin E; D010619:Phenazines; D001619:beta-N-Acetylhexosaminidases; C009195:safranine T