Peptide antibodies to the human c-fyn gene product demonstrate pp59c-fyn is capable of complex formation with the middle-T antigen of polyomavirus.
The c-fyn proto-oncogene is a member of a family of closely related genes of which c-src is the prototype. Using peptide antibodies which had been raised against sequences predicted to be specific for the human c-fyn gene product, the c-fyn protein was identified. It is a tyrosine kinase with apparent mol. wt of 59 kd that is also phosphorylated and myristylated. Like pp60c-src and pp62c-yes, pp59c-fyn is able to form a stable complex with middle-T antigen, the transforming protein of polyomavirus. The transformation-defective middle-T mutant NG59, which is unable to associate stably with pp60c-src does not associate with pp59c-fyn. In contrast to pp60c-src, complex formation with middle-T antigen does not lead to a significant increase in the tyrosine kinase activity of pp59c-fyn. These findings lead us to suggest that middle-T mediated transformation may be a consequence of the deregulation of several members of the src-family of protein tyrosine kinases.
Cheng
S H
SH
Laboratory of Cellular Regulation, Integrated Genetics Inc., Framingham, MA 01701.
Harvey
R
R
Espino
P C
PC
Semba
K
K
Yamamoto
T
T
Toyoshima
K
K
Smith
A E
AE
D000818:Animals; D000918:Antibody Specificity; D000952:Antigens, Polyomavirus Transforming / Q000378:metabolism*; D015153:Blotting, Western; D002472:Cell Transformation, Viral; D006801:Humans; D046911:Macromolecular Substances; D051379:Mice; D008970:Molecular Weight; D009226:Myristates / Q000032:analysis; D010449:Peptide Mapping; D010455:Peptides / Q000276:immunology; D010750:Phosphoproteins / Q000378:metabolism; D011120:Polyomavirus / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000276:immunology* / Q000378:metabolism; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000276:immunology* / Q000378:metabolism; D016392:Proto-Oncogene Proteins pp60(c-src); D014764:Viral Proteins / Q000276:immunology* / Q000378:metabolism
D000952:Antigens, Polyomavirus Transforming; C000715470:MAS1 protein, human; D046911:Macromolecular Substances; D009226:Myristates; D010455:Peptides; D010750:Phosphoproteins; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D014764:Viral Proteins; D011505:Protein-Tyrosine Kinases; D016392:Proto-Oncogene Proteins pp60(c-src)