Cyclic AMP calcium and the growth of mastocytoma cells.
Arresting P815 mastocytoma cell growth with N6, O2'-dibutyryladenosine 3':5' cyclic monophosphate (db cAMP) and theophylline increased 45Ca2+ uptake and efflux by the cells (i.e, Ca2+ cycling) without altering cytoplasmic free Ca2+ concentrations or the amount or distribution of protein kinase C in the cells. Attempts to identify the Ca2+ channels involved using a wide variety of drugs were unsuccessful. However, the inhibitory effect of db cAMP on growth was greatly increase in medium containing low Ca2+ concentrations, confirming that interactions between Ca2+ and cyclic AMP can affect mastocytoma cell growth.
Lints
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Department of Cellular and Molecular Biology, University of Auckland, New Zealand.
Holland
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Ralph
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D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000502:physiology*; D015220:Calcium Channels / Q000502:physiology; D002132:Calcium Radioisotopes; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D003593:Cytoplasm / Q000378:metabolism; D004533:Egtazic Acid / Q000494:pharmacology; D007811:Lanthanum / Q000494:pharmacology; D012515:Mast-Cell Sarcoma / Q000473:pathology*; D051379:Mice; D011493:Protein Kinase C / Q000378:metabolism; D012430:Ruthenium Red / Q000494:pharmacology; D013806:Theophylline / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014638:Vanadates / Q000494:pharmacology
D015220:Calcium Channels; D002132:Calcium Radioisotopes; C028521:lanthanum chloride; D012430:Ruthenium Red; D000001:Calcimycin; D014638:Vanadates; D004533:Egtazic Acid; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D003994:Bucladesine; D007811:Lanthanum; D013806:Theophylline; D011493:Protein Kinase C; D000252:Calcium-Transporting ATPases; D002118:Calcium