title: Bovine viral diarrhea virus proteins: heterogeneity of cytopathogenic and non-cytopathogenic strains and evidence of a 53K glycoprotein neutralization epitope.
abstract: Intracellular virus-induced polypeptides from 3 cytopathogenic and 2 non-cytopathogenic bovine viral diarrhea (BVD) virus reference strains were analyzed by radioimmunoprecipitation and polyacrylamide gel electrophoresis, using a specific bovine multivalent antiserum and a neutralizing BVD-virus monoclonal antibody. Electrophoretic patterns of major proteins demonstrate extensive variation between strains. Most notably, a major 80,000 (80K) polypeptide was present in all cytopathogenic strains but absent in both non-cytopathogenic strains. Furthermore, a neutralizing monoclonal antibody produced against the NADL strain immunoprecipitated a 53K glycoprotein indicating that this protein carries an important neutralization epitope that is not present in all strains tested.
authors: Centre de recherche en virologie, Institut Armand-Frappier, Université du Québec, Laval-des-Rapides, Canada.
authors: R
authors: R
authors: Magar
authors: H C
authors: HC
authors: Minocha
authors: J
authors: J
authors: Lecomte
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D000940:Antigenic Variation; D000956:Antigens, Viral / Q000032:analysis*; D001908:Diarrhea Viruses, Bovine Viral / Q000032:analysis* / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D005455:Fluorescent Antibody Technique; D007118:Immunoassay; D009500:Neutralization Tests; D010576:Pestivirus / Q000032:analysis*; D014764:Viral Proteins / Q000032:analysis* / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D000939:Epitopes; D014764:Viral Proteins