Extraction of peptides from guanidine thiocyanate homogenates used to isolate RNA.
A method is described in which the concentrated tissue extract that remains after preparation of RNA in guanidine thiocyanate can be salvaged for simultaneous measurements of neuropeptides by RIA and other neuroactive factors by bioassay. This procedure may be used for assessing alterations in tissue peptide content in addition to mRNA changes in the same tissue sample.
Iadarola
M J
MJ
Neurobiology and Anesthesiology Branch, National Institute of Dental Research, Bethesda, MD 20892.
Naranjo
J R
JR
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004399:Dynorphins / Q000302:isolation & purification; D019791:Guanidine; D006146:Guanidines; D007249:Inflammation; D009479:Neuropeptides / Q000302:isolation & purification*; D010446:Peptide Fragments / Q000302:isolation & purification; D012313:RNA / Q000302:isolation & purification*; D051381:Rats; D013116:Spinal Cord / Q000032:analysis*
D006146:Guanidines; D009479:Neuropeptides; D010446:Peptide Fragments; D012313:RNA; D004399:Dynorphins; C026770:dynorphin (1-8); D019791:Guanidine