Soluble bovine galactose-binding lectin. cDNA cloning reveals the complete amino acid sequence and an antigenic relationship with the major encephalitogenic domain of myelin basic protein.
A full-length cDNA clone for the 13-14 kDa soluble beta-galactoside-binding lectin was isolated from a bovine fibroblast cDNA library. The derived amino acid sequence shows eight differences from a preliminary partial amino acid sequence given previously for the bovine heart lectin. This observation led to a re-examination of the data and correction of the heart lectin protein sequence. Except for a possible polymorphism of the heart lectin at position 57, the fibroblast and heart lectin sequences are considered identical. The epitope recognized by two monoclonal anti-(bovine lectin) antibodies, 36/8 and 9/5, was identified as the tetrapeptide sequence W-G-A/S-E/D by the isolation of several different cDNA clones from a human intestine cDNA library. A similar tetrapeptide is present in all of the soluble beta-galactoside-binding animal lectins sequenced thus far. It is also found in myelin basic protein, which we show is antigenically cross-reactive with the lectin. In myelin basic protein the tetrapeptide is a part of the major domain previously shown to be responsible for the induction of experimental allergic encephalomyelitis.
Abbott
W M
WM
Section of Glycoconjugate Research, M.R.C. Clinical Research Centre, Harrow, Middx., U.K.
Mellor
A
A
Edwards
Y
Y
Feizi
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000276:immunology; D002417:Cattle; D003001:Cloning, Molecular; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology* / Q000302:isolation & purification; D005697:Galactosides / Q000378:metabolism; D037161:Galectins; D006388:Hemagglutinins / Q000276:immunology*; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000276:immunology; D004676:Myelin Basic Protein / Q000276:immunology*; D012995:Solubility
D002352:Carrier Proteins; D000939:Epitopes; D005697:Galactosides; D037161:Galectins; D006388:Hemagglutinins; D009124:Muscle Proteins; D004676:Myelin Basic Protein