Practical application of the protein C activator Protac from Agkistrodon contortrix venom.
The protein C activator Protac from A. contortrix venom is being investigated as a potential antithrombotic agent and as a tool for the preparation of activated protein C. Its established major application is the zymogen activation in functional protein C determinations based on either a clotting assay or a chromogenic substrate technique. The sensitivity of the activated partial thromboplastin time as an indicator reaction for Protac activated protein C depends on the contact activator component of the reagent. Protein C dose-response increased in the following order: kaolin greater than ellagic acid greater than sulfatide. This phenomenon is due to a competition of molecular affinities between Protac, plasma components and the different activating surfaces.
Stocker
K
K
Pentapharm Ltd., Basle, Switzerland.
Fischer
H
H
Meier
J
J
D003435:Crotalid Venoms; D005343:Fibrinolytic Agents; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D011486:Protein C / Q000378:metabolism*
D003435:Crotalid Venoms; D005343:Fibrinolytic Agents; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D011486:Protein C; C049647:snake venom protein C activator