The inhibition of human adrenal steroidogenic enzyme activities by suramin.
Suramin has recently been used to treat patients with acquired immune deficiency syndrome because of the action of this drug on reverse transcriptase. Patients so treated developed the symptoms and hormonal profiles of adrenal insufficiency. To evaluate the mechanism of action of suramin on adrenalcortical function, adrenal mitochondrial and microsomal preparations from five subjects were assayed for steroidogenic enzyme activity in the presence and absence of suramin. Specifically, 3 beta-hydroxysteroid dehydrogenase/isomerase, 17 alpha-hydroxylase, 21-hydroxylase, 11 beta-hydroxylase, and 17,20-desmolase activities were measured in the presence of 0-5000 mumol/L suramin concentrations. In all assays, enzyme activities decreased in a dose-dependent fashion as suramin concentrations increased. The drug doses (calculated) that caused 50% inhibition of enzyme activity were: 21-hydroxylase activity, 50 mumol/L; 17 alpha-hydroxylase activity, 25 mumol/L; 17,20-desmolase activity, 50 mumol/L; 11 beta-hydroxylase, 2 mumol/L, and 3 beta-hydroxysteroid dehydrogenase/isomerase, 1200 mumol/L. These results suggest that suramin has a concentration-dependent inhibitory effect on the key P-450-regulated enzymatic steps in adrenal glucocorticoid steroidogenesis, which may explain the development of adrenal insufficiency in acquired immune deficiency syndrome patients treated with suramin.
Ashby
H
H
Department of Obstetrics and Gynecology, Georgetown University Medical Center, Washington, D.C. 20007.
DiMattina
M
M
Linehan
W M
WM
Robertson
C N
CN
Queenan
J T
JT
Albertson
B D
BD
D000163:Acquired Immunodeficiency Syndrome / Q000201:enzymology; D000302:Adrenal Cortex / Q000187:drug effects / Q000201:enzymology* / Q000502:physiology; D006801:Humans; D006913:Hydroxysteroid Dehydrogenases / Q000037:antagonists & inhibitors; D008861:Microsomes / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D018894:Reverse Transcriptase Inhibitors; D013250:Steroid Hydroxylases / Q000037:antagonists & inhibitors; D013251:Steroid Isomerases / Q000037:antagonists & inhibitors; D013498:Suramin / Q000009:adverse effects / Q000494:pharmacology*
D018894:Reverse Transcriptase Inhibitors; D013498:Suramin; D006913:Hydroxysteroid Dehydrogenases; D013250:Steroid Hydroxylases; D013251:Steroid Isomerases