Cryopreservation of marrow, purging and autologous bone marrow transplantation in childhood.
Since 1984 bone marrow from 42 children with acute lymphoblastic leukaemia, non Hodgkin's lymphoma and neuroblastoma was cryopreserved. In 5 cases (c-ALL, NHL and B type) the marrow was purged by using a cocktail of three monoclonal antibodies (VIL A1, VIB C5, VIB-E3). Up to now 13 children (ALL/10, neuroblastoma/3) were autografted (one of them after purging) after supralethal chemoradiotherapy. Except one child with early death all patients had engraftment: a level of 1.0.10(9)/l leukocytes was reached at days 10-33 (median, 19); platelet level over 60.10(9)/l at days 32-60 (median, 41). 2 children died on treatment related complications, one on infection after full haematological restitution, 2 patients alive with relapse, 8/13 alive in CCR and well.
Reiners
B
B
Department of Haematology/Oncology, Children's University Hospital, Jussuf Ibrahim, Jena.
Hermann
J
J
Fuchs
D
D
Prager
J
J
Thränhardt
H
H
Burger
E
E
Kob
D
D
Zintl
F
F
D000911:Antibodies, Monoclonal; D001853:Bone Marrow / Q000473:pathology; D016026:Bone Marrow Transplantation; D002469:Cell Separation; D002648:Child; D002675:Child, Preschool; D015925:Cryopreservation; D006098:Granulocytes / Q000473:pathology; D006412:Hematopoietic Stem Cells / Q000473:pathology; D006801:Humans; D007223:Infant; D007938:Leukemia / Q000473:pathology / Q000601:surgery; D008228:Lymphoma, Non-Hodgkin / Q000473:pathology / Q000601:surgery; D008264:Macrophages / Q000473:pathology; D009447:Neuroblastoma / Q000473:pathology / Q000601:surgery; D012208:Rhabdomyosarcoma / Q000473:pathology / Q000601:surgery; D014021:Tissue Preservation; D014182:Transplantation, Autologous
D000911:Antibodies, Monoclonal