title: Resting plasma membrane potential of rat peritoneal mast cells is set predominantly by the sodium pump.
abstract: Changes in the plasma membrane potential of two histamine-releasing cells, rat peritoneal mast cells and basophilic leukemia cells 2H3 (RBL 2H3), were recorded with the potential-sensitive dye bis-oxonol. For mast cells, the presence of ouabain or the absence of K+ increased the fluorescence intensity of bis-oxonol; gramicidin had no effect. For RBL 2H3 cells, the presence of ouabain and the absence of K+ also increased bis-oxonol fluorescence but gramicidin also increased it. These results show that the plasma membrane potential of RBL 2H3 cells is set, in part, by the activity of the Na+ pump and in part by the K+ conductance, while that of rat mast cells is set predominantly by the Na+ pump.
authors: Laboratoire de Neuroimmunopharmacologie, Université Louis Pasteur Strasbourg I, Illkirch, France.
authors: C
authors: C
authors: Bronner
authors: M
authors: M
authors: Mousli
authors: N
authors: N
authors: Eleno
authors: Y
authors: Y
authors: Landry
mesh_terms: D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000494:pharmacology; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D006096:Gramicidin / Q000494:pharmacology; D007700:Kinetics; D015471:Leukemia, Basophilic, Acute; D008297:Male; D008407:Mast Cells / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012964:Sodium / Q000378:metabolism*
chemical_list: D006096:Gramicidin; D010042:Ouabain; D012964:Sodium; D011188:Potassium; D002118:Calcium