S-100a0 protein stimulates Ca2+-induced Ca2+ release from isolated sarcoplasmic reticulum vesicles.
S-100a0 protein, the alpha alpha-isoform of the S-100 family, stimulates Ca2+-induced Ca2+ release from terminal cisternae isolated from rat skeletal muscle cells. The stimulatory effect of S-100a0 is maximal at approximately 5 microM S-100a0 and half maximal at approximately 0.1 microM S-100a0, at 1.8 microM free Ca2+ in the presence of 5 mM Mg2+ plus 0.1 M KCl. The effect of the protein on Ca2+-induced Ca2+ release is completely inhibited by the calcium release blocker, ruthenium red.
Fanò
G
G
Institute of Cell Biology, Faculty of Sciences, University of Perugia, Italy.
Marsili
V
V
Angelella
P
P
Aisa
M C
MC
Giambanco
I
I
Donato
R
R
D000818:Animals; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D006321:Heart / Q000502:physiology; D007700:Kinetics; D009132:Muscles / Q000378:metabolism; D051381:Rats; D012430:Ruthenium Red / Q000494:pharmacology; D009418:S100 Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D012519:Sarcoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism*; D013552:Swine
D009418:S100 Proteins; D012430:Ruthenium Red; D002118:Calcium