title: Interactions in vitro and in vivo between human and porcine cationic pancreatic elastase and plasma protease inhibitors.
abstract: Trace amounts of porcine pancreatic elastase mixed with porcine serum, or injected intravenously into the pig, were found to be bound mainly to alpha 1- and alpha 2-macroglobulin (90%). Alpha 1-macroglobulin approached saturation with elastase before significant binding to alpha 2-macroglobulin was demonstrable. Human pancreatic cationic elastase showed in human serum preferential binding to alpha 2-macroglobulin, but the elastase was also bound by alpha 1-protease inhibitor and by alpha 1-antichymotrypsin. The porcine elastase-alpha 1 alpha 2-macroglobulin complexes injected intravenously or formed in vivo in the pig were rapidly eliminated from the blood stream following a first order reaction with t 1/2 = 8 min. Porcine alpha 1-protease-inhibitor-bound elastase disappeared considerably more slowly.
authors: Department of Surgery, University of Lund, Malmö General Hospital, Sweden.
authors: H O
authors: HO
authors: Håkansson
authors: K
authors: K
authors: Ohlsson
mesh_terms: D000818:Animals; D005260:Female; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D007700:Kinetics; D008297:Male; D010179:Pancreas / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000378:metabolism*; D011480:Protease Inhibitors / Q000097:blood* / Q000378:metabolism; D011485:Protein Binding; D013552:Swine; D000511:alpha-Macroglobulins / Q000378:metabolism
chemical_list: D011480:Protease Inhibitors; D000511:alpha-Macroglobulins; D010196:Pancreatic Elastase