title: Electron microscopy and image analysis of the multicatalytic proteinase.
abstract: One electron micrographs, negatively stained multicatalytic proteinase molecules are viewed end-on (ring shaped) or side-on (rectangular shaped). For aurothioglucose, ammonium molybdate- and phosphotungstate-stained molecules, the dimensions measured are consistent. In contrast, uranyl acetate-staining reveals ring-shaped particles which vary in diameter between 12 and 16 nm. This is due to a partial collapse and substantial flattening of the structure. Digital image analysis of side-on views of the particles reveals a tripartite, reel-shaped structure. Within the ring-like, end-on projections of ammonium molybdate-stained molecules six local centres of mass can be discerned; their position appears to depart, however, from a true six-fold symmetry.
authors: Max-Planck-Institut für Biochemie, Martinsried, FRG.
authors: W
authors: W
authors: Baumeister
authors: B
authors: B
authors: Dahlmann
authors: R
authors: R
authors: Hegerl
authors: F
authors: F
authors: Kopp
authors: L
authors: L
authors: Kuehn
authors: G
authors: G
authors: Pfeifer
mesh_terms: D000818:Animals; D003546:Cysteine Endopeptidases / Q000302:isolation & purification; D008854:Microscopy, Electron; D009097:Multienzyme Complexes / Q000302:isolation & purification; D009132:Muscles / Q000201:enzymology; D046988:Proteasome Endopeptidase Complex; D051381:Rats; D013194:Staining and Labeling
chemical_list: D009097:Multienzyme Complexes; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex