Characterization of DNA polymerase beta mRNA: cell-cycle and growth response in cultured human cells.
DNA polymerase beta (beta-polymerase) is a housekeeping enzyme involved in DNA repair in vertebrate cells. We used a cDNA probe to study abundance of beta-polymerase mRNA in cultured human cells. The mRNA level in synchronized HeLa cells, representing different stages of the cell-cycle, varied only slightly. Contact inhibited fibroblasts AG-1522 contained the same level of mRNA as growing cells. The steady-state level of mRNA in fibroblasts is equivalent to 6 molecules per cell. The results indicate that the beta-polymerase transcript is "low abundance" and is neither cell-cycle nor growth phase responsive.
Zmudzka
B Z
BZ
Laboratory of Biochemistry, National Cancer Institute, Bethesda, MD 20892.
Fornace
A
A
Collins
J
J
Wilson
S H
SH
D002453:Cell Cycle; D002455:Cell Division; D003260:Contact Inhibition; D004256:DNA Polymerase I / Q000302:isolation & purification / Q000378:metabolism*; D005347:Fibroblasts / Q000201:enzymology / Q000378:metabolism / Q000502:physiology; D006367:HeLa Cells / Q000201:enzymology* / Q000378:metabolism / Q000502:physiology; D006801:Humans; D007399:Interphase; D011061:Poly A / Q000302:isolation & purification; D012313:RNA / Q000302:isolation & purification; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004256:DNA Polymerase I