Immunological characterization of proteins detected by phosphotyrosine antibodies in cells transformed by Rous sarcoma virus.
Phosphotyrosine antibodies were used to identify tyrosine-phosphorylated proteins in Rous sarcoma virus (RSV)-transformed chicken embryo fibroblasts. A large number of tyrosine phosphoproteins were detected. A similar set of proteins was observed in RSV-transformed murine cells. An 85,000-dalton protein, however, was present in transformed avian cells but missing in transformed murine cells. Neither the 85,000-dalton protein nor any of the other tyrosine phosphoproteins appeared to be viral structural proteins. Use of RSV mutants encoding partially deleted src gene products enabled us to identify a 60,000-dalton cellular tyrosine phosphoprotein that comigrated with wild-type pp60v-src. With the exception of calpactin I, the major tyrosine phosphoproteins detected in immunoblots appeared to be different from several previously characterized substrates of pp60v-src with similar molecular masses (ezrin, vinculin, and the fibronectin receptor).
Linder
M E
ME
Program in Molecular Biology, University of Texas, Dallas, Richardson 75083-0688.
Burr
J G
JG
D017302:Annexins; D001358:Avian Sarcoma Viruses; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002460:Cell Line; D002472:Cell Transformation, Viral; D003598:Cytoskeletal Proteins; D008970:Molecular Weight; D009124:Muscle Proteins / Q000378:metabolism; D015688:Oncogene Protein pp60(v-src); D010750:Phosphoproteins / Q000276:immunology* / Q000378:metabolism; D019000:Phosphotyrosine; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic / Q000378:metabolism; D012191:Retroviridae Proteins / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000276:immunology; D016596:Vinculin; D000099149:Ezrin
D017302:Annexins; D002135:Calcium-Binding Proteins; D003598:Cytoskeletal Proteins; D009124:Muscle Proteins; D015688:Oncogene Protein pp60(v-src); D010750:Phosphoproteins; D019000:Phosphotyrosine; D017469:Receptors, Fibronectin; D011971:Receptors, Immunologic; D012191:Retroviridae Proteins; D014443:Tyrosine; D016596:Vinculin; D000099149:Ezrin