Rapid determination of bacterial ribosomal RNA sequences by direct sequencing of enzymatically amplified DNA.
Ribosomal RNA sequences are an appealing target for bacterial classification as well as for development of group- or species-specific DNA probes. Using the polymerase chain reaction and synthetic primers, the feasibility of this gene amplification technique for rapid sequence determination of the major 16S ribosomal RNA domains from small amounts of input DNA is demonstrated. Information useful for phylogenetic classification as well as for construction of specific DNA probes may be obtained by comparison with known sequences.
Böttger
E C
EC
Medizinische Hochschule Hannover, Institut für Medizinische Mikrobiologie, F.R.G.
D001419:Bacteria / Q000145:classification* / Q000235:genetics; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000145:classification / Q000235:genetics; D005784:Gene Amplification; D007875:Legionella / Q000145:classification / Q000235:genetics; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S