Reversion from erythromycin dependence in Escherichia coli: strains altered in ribosomal sub-unit association and ribosome assembly.
A mutant of Escherichia coli dependent on erythromycin for growth spontaneously gives erythromycin-independent strains with altered or missing ribosomal proteins. strains with defects in ribosome assembly were sought and obtained from among these revertants. Two organisms in which ribosomal protein L19 is altered and absent respectively have 70S ribosomes whose dissociation into sub-units is particularly sensitive to pressures generated during centrifuging. The mutant that lacks protein L19 also accumulates ribosome precursor particles during exponential growth as do others including mutants that lack proteins S20 or L1. These strains also show unbalanced synthesis of RNA and so will be useful in investigating both the pathways and the regulation of ribosome assembly.
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Department of Biochemistry, University of Oxford, UK.
D001426:Bacterial Proteins / Q000032:analysis; D002499:Centrifugation, Density Gradient; D004917:Erythromycin / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics*; D046911:Macromolecular Substances; D009154:Mutation; D012329:RNA, Bacterial / Q000032:analysis; D012335:RNA, Ribosomal / Q000032:analysis; D012261:Ribonucleoproteins / Q000378:metabolism; D012269:Ribosomal Proteins / Q000032:analysis; D012270:Ribosomes / Q000378:metabolism*
D001426:Bacterial Proteins; D046911:Macromolecular Substances; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012261:Ribonucleoproteins; D012269:Ribosomal Proteins; D004917:Erythromycin