Decreased density of alpha 2-adrenoceptors in medulla oblongata of spontaneously hypertensive rats.
To study the role of medullary alpha-adrenoceptors in hypertension, we compared specific binding of [3H]prazosin and [3H]clonidine in different brain regions of spontaneously hypertensive rats (SHR), stroke-prone SHR (SHRSP), and normotensive Wistar-Kyoto rats (WKY). As compared with age-matched WKY, Bmax values for specific [3H]clonidine binding in the medulla oblongata were significantly lower in SHR and SHRSP at 16-24 weeks of age. In the SHRSP medulla oblongata, the decrease was more prominent in dorsomedial and ventrolateral regions than in the ventromedial region. Density of alpha 2-adrenoceptor binding sites was also decreased in the medulla oblongata of young (4-5-week-old) SHRSP. In contrast, there was no difference in Kd and Bmax values for medullary [3H]prazosin binding between WKY and SHRSP. The dorsomedial and ventrolateral regions of the SHRSP medulla oblongata showed significantly lower levels of norepinephrine (NE). Thus, the present study demonstrates that there is a specific loss of alpha 2-adrenoceptors in the medulla oblongata of SHR and SHRSP that may be partly involved in the pathogenesis of spontaneous hypertension.
Yamada
S
S
Department of Pharmacology, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
Ashizawa
N
N
Nakayama
K
K
Tomita
T
T
Hayashi
E
E
D000818:Animals; D001665:Binding Sites; D003000:Clonidine / Q000494:pharmacology; D006973:Hypertension / Q000378:metabolism*; D008526:Medulla Oblongata / Q000032:analysis* / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism; D010646:Phentolamine / Q000494:pharmacology; D011224:Prazosin / Q000494:pharmacology; D051381:Rats; D011918:Rats, Inbred SHR; D011919:Rats, Inbred Strains; D011942:Receptors, Adrenergic, alpha / Q000032:analysis*; D012436:S-Adenosylmethionine / Q000494:pharmacology
D011942:Receptors, Adrenergic, alpha; D012436:S-Adenosylmethionine; D003000:Clonidine; D009638:Norepinephrine; D011224:Prazosin; D010646:Phentolamine