[Modification of latrotoxin channels under the effect of pronase and antibodies to latrotoxin].
The action of pronase and latrotoxin antibodies on alpha-latrotoxin channels in lipid bilayers has been investigated. Proteolysis of the protruding portions of the inserted channels changed slightly its conductivity and selectivity. Latrotoxin antibodies added to either side of the membrane decreased channel conductivity. The effect depends on the membrane potential. It is suggested that the channel conformation depends on the transmembrane potential and selective centre localized in the inner region of a bilayer is insensitive to proteolysis of protruding segments.
Chanturiia
A N
AN
Shaturskiĭ
O Ia
OIa
Nikolaenko
A N
AN
Lishko
V K
VK
D000906:Antibodies / Q000032:analysis*; D001180:Arthropod Venoms / Q000378:metabolism*; D006868:Hydrolysis; D007473:Ion Channels / Q000378:metabolism*; D007527:Isoenzymes; D008051:Lipid Bilayers; D008564:Membrane Potentials; D011402:Pronase / Q000378:metabolism*; D013111:Spider Venoms / Q000378:metabolism*
D000906:Antibodies; D001180:Arthropod Venoms; D007473:Ion Channels; D007527:Isoenzymes; D008051:Lipid Bilayers; D013111:Spider Venoms; C020763:alpha-latrotoxin; D011402:Pronase
Modifikatsiia latrotoksinovykh kanalov pod vliianiem pronazy i antitel k latrotoksinu.