Inhibition of trypsin-like enzymes on cells with rhodamine-aprotinin.
Aprotinin, a polypeptide inhibitor of trypsin-like enzymes, has been labelled with rhodamine. Rhodamine-aprotinin inhibits trypsin in free solution in an identical manner to aprotinin. Rhodamine-aprotinin binds to trypsin-like enzymes on cells in formaldehyde fixed wax embedded sections. This technique has been used to locate cells possessing trypsin-like enzymes by means of fluorescent microscopy. In the present study we have used this technique to locate tumour cells.
Steven
F S
FS
Department of Biochemistry, School of Biological Sciences, University of Manchester, U.K.
Griffin
M M
MM
Williams
L A
LA
Freemont
A J
AJ
Maier
H
H
Weidauer
H
H
D000818:Animals; D007611:Aprotinin / Q000494:pharmacology*; D005557:Formaldehyde; D006651:Histocytochemistry; D006801:Humans; D007700:Kinetics; D008856:Microscopy, Fluorescence / Q000379:methods; D009369:Neoplasms / Q000201:enzymology* / Q000473:pathology; D011485:Protein Binding; D051381:Rats; D012235:Rhodamines / Q000494:pharmacology*; D014357:Trypsin / Q000378:metabolism; D014361:Trypsin Inhibitors / Q000494:pharmacology*; D014966:Xanthenes / Q000494:pharmacology*
D012235:Rhodamines; D014361:Trypsin Inhibitors; D014966:Xanthenes; D005557:Formaldehyde; D007611:Aprotinin; D014357:Trypsin