Idiotype-specific regulation might contribute to specific unresponsiveness in dextran-primed mice.
Priming of adult responder mice with optimal immunogenic doses of dextran alpha, 1-6 results in reduced antibody responses to secondary antigenic challenge. We have now quantitated dextran-specific clonal precursors in the large and small B-lymphocyte compartments within several days or several months after priming with either dextran or antiidiotypic antibodies directed to a dominant idiotype of C57BL/6 mice which accounts for more than 50% of the antibody response. The results show that "secondary unresponsiveness" correlates with idiotype-directed depletion of the appropriate specificities from the immunocompetent resting B-cell pool.
Lundkvist
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I
Unit for Applied Cell and Molecular Biology, Umeå University, Sweden.
Portnoi
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D
Coutinho
A
A
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000008:administration & dosage; D000917:Antibody Formation; D000941:Antigens / Q000008:administration & dosage; D003911:Dextrans / Q000276:immunology*; D007108:Immune Tolerance; D007117:Immunization, Secondary; D007130:Immunoglobulin Idiotypes / Q000096:biosynthesis; D008212:Lymphocyte Depletion; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats
D000888:Antibodies, Anti-Idiotypic; D000941:Antigens; D003911:Dextrans; D007130:Immunoglobulin Idiotypes