title: Patterns of keratin-expression in rests of Malassez and periapical lesions.
abstract: Using immunocytochemistry and a panel of monoclonal antibodies directed against various keratin polypeptides we examined specimens of normal periodontal ligament, periapical granulomas and inflammatory dental cysts. Epithelial elements with the appearance of rests of Malassez were identified in 6 specimens of normal periodontium and 10 periapical granulomas. Altered epithelium was present in 16 periapical granulomas and a lining epithelium in 10 inflammatory dental cysts. The patterns of binding of antibodies by these epithelia indicated that (a) keratin 19 was expressed by all epithelia, and (b) rests of Malassez also expressed keratin 5 but not large amounts of other keratins and (c) epithelial proliferation in periapical lesions was associated with increased expression of keratin 14, a marker of stratifying epithelia, new expression of keratins 4 and 13, differentiation markers for non-cornifying epithelia and variable, low levels of keratins 8 and 18, markers of simple epithelia. Proliferation of the epithelial rests of Malassez to form the lining of inflammatory dental cysts thus appears to be associated with a change from an unusual epithelial phenotype to that of a stratified non-cornifying epithelium in which some simple epithelial keratins are coexpressed.
authors: Dows Institute for Dental Research, University of Iowa, Iowa City 52242.
authors: Z
authors: Z
authors: Gao
authors: I C
authors: IC
authors: Mackenzie
authors: D M
authors: DM
authors: Williams
authors: A T
authors: AT
authors: Cruchley
authors: I
authors: I
authors: Leigh
authors: E B
authors: EB
authors: Lane
mesh_terms: D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal; D005819:Genetic Markers; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis* / Q000145:classification / Q000235:genetics; D010484:Periapical Granuloma / Q000378:metabolism*; D010513:Periodontal Ligament / Q000032:analysis*; D010641:Phenotype; D011842:Radicular Cyst / Q000378:metabolism*; D013194:Staining and Labeling
chemical_list: D000911:Antibodies, Monoclonal; D005819:Genetic Markers; D007633:Keratins