title: Interaction of RNA polymerase II with structurally altered nucleosomal particles. Transcription is facilitated by loss of one H2A.H2B dimer.
abstract: The loss of one H2A.H2B dimer from the nucleosomal core increases its affinity for RNA polymerase II and its efficiency as a transcription template, allowing transcription of the entire DNA present in the particle. In contrast, the nucleosomal core lacking the amino-terminal ends of histones, which has an affinity for polymerase equal to that of the H2A.H2B-deficient core, shows transcription properties similar to those of the whole nucleosomal core, with synthesis of short RNA chains (40 nucleotides or less). Similar results were obtained with a bacterial RNA polymerase. The improved efficiency of the H2A.H2B-deficient cores as transcription templates does not appear to be produced by nonspecific loss of protein or structural relaxation of the particle. These results suggest that a particle lacking one H2A.H2B dimer might be a necessary intermediate during in vivo transcription.
authors: Centro de Biología Molecular, Universidad Autónoma de Madrid, Spain.
authors: P J
authors: PJ
authors: González
authors: E
authors: E
authors: Palacián
mesh_terms: D000818:Animals; D002645:Chickens; D004247:DNA / Q000378:metabolism; D004912:Erythrocytes / Q000648:ultrastructure*; D004926:Escherichia coli / Q000201:enzymology; D006657:Histones / Q000502:physiology*; D046911:Macromolecular Substances; D009707:Nucleosomes / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis; D012319:RNA Polymerase II / Q000378:metabolism*; D013698:Templates, Genetic; D014158:Transcription, Genetic / Q000502:physiology*
chemical_list: D006657:Histones; D046911:Macromolecular Substances; D009707:Nucleosomes; D012313:RNA; D004247:DNA; D012319:RNA Polymerase II