Protein-RNA crosslinking in the subunits of the cytoplasmic ribosome of Tetrahymena thermophila.
Use of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide to introduce RNA-protein crosslinks in the 40S and 60S subunits of the cytoplasmic ribosome of Tetrahymena thermophila is described, and proteins linked covalently to 17S and 26S ribosomal RNAs are identified. RNA-protein crosslinking is accompanied by extensive dimerization and aggregation of ribosomal subunits probably due to formation of interparticle protein-protein crosslinks.
Petridou
B
B
Laboratoire de Chimie Cellulaire, Institut de Biologie Physico-Chimique, Paris, France.
Guerin
M F
MF
Hayes
F
F
D000818:Animals; D002499:Centrifugation, Density Gradient; D003432:Cross-Linking Reagents; D003593:Cytoplasm / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005022:Ethyldimethylaminopropyl Carbodiimide; D010449:Peptide Mapping; D012313:RNA / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000378:metabolism*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D013768:Tetrahymena / Q000187:drug effects / Q000378:metabolism*
D003432:Cross-Linking Reagents; D012269:Ribosomal Proteins; C041308:ribosomal protein S40; D012313:RNA; D005022:Ethyldimethylaminopropyl Carbodiimide