Properties of acetylcholine-receptor activation in human Duchenne muscular dystrophy myotubes.
In human myotubes cultured from biopsies of normal subjects and dystrophic patients we investigated, with the patch-clamp technique, the activation properties of the nicotinic acetylcholine receptor (AChoR) in the presence of acetylcholine and suberyldicholine. The single-channel conductance and the lifetime of the openings were not found to differ. In contrast, the average frequency of openings was about four times higher in Duchenne muscular dystrophy (DMD) myotubes in the presence of equal amounts of acetylcholine, but not of suberyldicholine. The most reasonable conclusion from this observation is that the behaviour of the AChoR is not altered in DMD cells but that there is a greater average concentration of ACho molecules present around AChoRs. This leads to the tentative conclusion that the activity of the enzyme acetylcholinesterase (AChoE) is impaired by some unknown mechanism in the dystrophic myotube.
Mancinelli
E
E
Department of General Physiology and Biochemistry, University of Milan, Italy.
Sardini
A
A
D'Aumiller
A
A
Meola
G
G
Martucci
G
G
Cossu
G
G
Wanke
E
E
D000109:Acetylcholine / Q000494:pharmacology*; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D002794:Choline / Q000031:analogs & derivatives* / Q000494:pharmacology; D004553:Electric Conductivity; D006801:Humans; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D008564:Membrane Potentials / Q000187:drug effects; D009132:Muscles / Q000187:drug effects / Q000502:physiology / Q000503:physiopathology*; D009136:Muscular Dystrophies / Q000503:physiopathology*; D011978:Receptors, Nicotinic / Q000187:drug effects / Q000502:physiology*; D012016:Reference Values
D007473:Ion Channels; D011978:Receptors, Nicotinic; C009335:subecholine; D002794:Choline; D000109:Acetylcholine