Isolation of endo A cDNA from mouse 8-cell stage embryos.
To analyse the species of genes expressed in a cleavage stage mouse embryo, we have constructed a cDNA library containing 3.0 x 10(5) independent clones from about 2 x 10(3) embryos at the 8-cell stage of development. Endo A cDNA prepared from parietal yolk sac endoderm like PYS-2 cells was used to screen the library. Southern blot analyses using the endo A sequence as a probe and restriction mapping analyses revealed that four independent recombinants had been inserted endo A sequence. Sequencing data of these clones showed that endo A mRNA present in the 8-cell stage embryo is identical to that of parietal yolk sac endoderm cells.
Nozaki
M
M
Department of Microbial Genetics, Osaka University, Japan.
Murata
K
K
Morita
T
T
Matsushiro
A
A
D000818:Animals; D001483:Base Sequence; D001755:Blastocyst / Q000378:metabolism*; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D007633:Keratins / Q000235:genetics*; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D015017:Yolk Sac / Q000378:metabolism
D007633:Keratins; D004247:DNA