title: High initiation rates at the ribosomal gene promoter do not depend upon spacer transcription.
abstract: We report experiments that test the model that in Xenopus laevis, RNA polymerase I is "handed over" in a conservative fashion from the T3 terminator to the adjacent gene promoter. We have introduced transcription-terminating lesions into the ribosomal DNA repeat by irradiating cultured cells with ultraviolet light. We used isolated nuclei to measure the effect of such lesions on transcription. UV damage sufficient to prevent all elongating RNA polymerase from reaching T3 from upstream had no adverse effect on the density of RNA polymerase at the very 5' end of the gene. We conclude that high rates of transcription initiation at the gene promoter do not depend upon polymerase passing from one repeat to the next or on polymerase initiating at the spacer promoters.
authors: Hutchinson Cancer Research Center, Seattle, WA 98104.
authors: P
authors: P
authors: Labhart
authors: R H
authors: RH
authors: Reeder
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D015342:DNA Probes; D004275:DNA, Ribosomal / Q000378:metabolism* / Q000528:radiation effects; D006493:Heparin / Q000494:pharmacology; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D011401:Promoter Regions, Genetic; D012313:RNA / Q000235:genetics; D012318:RNA Polymerase I / Q000378:metabolism*; D012322:RNA Precursors / Q000235:genetics; D012521:Sarcosine / Q000031:analogs & derivatives / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects; D014466:Ultraviolet Rays; D014982:Xenopus laevis
chemical_list: D015342:DNA Probes; D004275:DNA, Ribosomal; D012322:RNA Precursors; D012313:RNA; C025231:sarkosyl; D006493:Heparin; D012318:RNA Polymerase I; D012521:Sarcosine