Vascular permeability factor, an endothelial cell mitogen related to PDGF.
Vascular permeability factor (VPF) is a 40-kilodalton disulfide-linked dimeric glycoprotein that is active in increasing blood vessel permeability, endothelial cell growth, and angiogenesis. These properties suggest that the expression of VPF by tumor cells could contribute to the increased neovascularization and vessel permeability that are associated with tumor vasculature. The cDNA sequence of VPF from human U937 cells was shown to code for a 189-amino acid polypeptide that is similar in structure to the B chain of platelet-derived growth factor (PDGF-B) and other PDGF-B-related proteins. The overall identity with PDGF-B is 18%. However, all eight of the cysteines in PDGF-B were found to be conserved in human VPF, an indication that the folding of the two proteins is probably similar. Clusters of basic amino acids in the COOH-terminal halves of human VPF and PDGF-B are also prevalent. Thus, VPF appears to be related to the PDGF/v-sis family of proteins.
Keck
P J
PJ
Department of Cell Culture and Biochemistry, Monsanto Company, St. Louis, MO 63167.
Hauser
S D
SD
Krivi
G
G
Sanzo
K
K
Warren
T
T
Feder
J
J
Connolly
D T
DT
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002199:Capillary Permeability / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D003001:Cloning, Molecular; D004730:Endothelium, Vascular / Q000166:cytology*; D006133:Growth Substances; D006168:Guinea Pigs; D006801:Humans; D008222:Lymphokines / Q000502:physiology*; D008969:Molecular Sequence Data; D009389:Neovascularization, Pathologic / Q000503:physiopathology; D020575:Oncogene Proteins v-sis; D010982:Platelet-Derived Growth Factor / Q000502:physiology; D015682:Retroviridae Proteins, Oncogenic / Q000502:physiology; D012689:Sequence Homology, Nucleic Acid; D015291:Transforming Growth Factors; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D006133:Growth Substances; D008222:Lymphokines; D020575:Oncogene Proteins v-sis; D010982:Platelet-Derived Growth Factor; D015682:Retroviridae Proteins, Oncogenic; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D015291:Transforming Growth Factors