Antigen presentation in an HLA-DR-restricted fashion by B-cell chronic lymphocytic leukemia cells.
The ability of B-cell chronic lymphocytic leukemia (B-CLL) cells to present antigen to antigen-specific T cells was investigated. B-CLL cells present herpes simplex virus (HSV) antigen and purified protein derivative (PPD) to HSV- and PPD-specific, interleukin-2-dependent T-cell lines in an antigen-specific manner. Treatment of B-CLL cells with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) induced markedly increased levels of HLA-DR expression. TPA-treated B-CLL cells showed substantially more effective presentation, especially at low antigen concentrations, than did untreated B-CLL cells. By coculturing different allogeneic combinations of B-CLL cells and T cells and by adding anti-HLA-DR monoclonal antibody to cultures, it was found that antigen presentation by B-CLL cells was restricted by HLA-DR in the same way as for macrophages. We concluded from these experiments that B-CLL cells have a capacity to serve as antigen-presenting cells in an HLA class II-restricted fashion and that increasing the amount of HLA class II antigen and activation of B-CLL cells resulted in effective antigen presentation.
Yasukawa
M
M
First Department of Internal Medicine, Ehime University School of Medicine, Japan.
Shiroguchi
T
T
Inatsuki
A
A
Kobayashi
Y
Y
D000911:Antibodies, Monoclonal / Q000502:physiology; D000938:Antigen-Presenting Cells / Q000187:drug effects / Q000276:immunology*; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002460:Cell Line; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes / Q000276:immunology; D006681:HLA-D Antigens / Q000032:analysis / Q000235:genetics / Q000276:immunology; D006684:HLA-DR Antigens / Q000032:analysis / Q000235:genetics / Q000276:immunology; D006801:Humans; D007166:Immunosuppressive Agents / Q000502:physiology; D007945:Leukemia, Lymphoid / Q000235:genetics / Q000276:immunology*; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D009000:Monocytes / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006681:HLA-D Antigens; D006684:HLA-DR Antigens; D007166:Immunosuppressive Agents; D013755:Tetradecanoylphorbol Acetate