Serologically defined linear epitopes in the envelope protein of dengue 2 (Jamaica strain 1409).
Antisera from dengue patients and dengue virus infected rabbits recognized octapeptides corresponding to linear amino acid sequences in the envelope protein of dengue 2 (Jamaica 1409). Although no peptide was recognized by sera from all dengue infected hosts, two peptides (216LPLPWLPG223 and 448FSGVSWTM455) were recognized by sera from all dengue 2 infected rabbits. One of these 448FSGVSWTM455 was also recognized by sera from both the dengue 2 patients tested. No peptides were identified which reacted exclusively with all dengue 2 infected animals. Use of a mouse monoclonal antibody (1B7) enabled identification of two regions (50AKQPATLR57 and 127GKVVLPEN134) and possibly a third (349GRLITVNP356) in the envelope protein of dengue 2 likely to be involved in haemagglutination inhibition and virus neutralization in vitro.
Aaskov
J G
JG
Faculty of Health Science, Queensland University of Technology, Melbourne, Victoria, Australia.
Geysen
H M
HM
Mason
T J
TJ
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D003715:Dengue / Q000276:immunology; D003716:Dengue Virus / Q000276:immunology*; D000939:Epitopes; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011817:Rabbits; D014759:Viral Envelope Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; C051821:E-glycoprotein, Dengue virus type 2; D000939:Epitopes; D014759:Viral Envelope Proteins