title: Pre-pre-B cells containing only cytoplasmic B220 can be induced by dendritic cells and T cells to differentiate in vitro.
abstract: We have derived from spleens of nude mice early B lineage cells that were phenotypically compatible with a pre-pre-B cell stage of differentiation. Although these cells containing large basophilic granules had the B lymphocyte antigen B220, in the cytoplasm, they had no surface B220, no cytoplasmic or surface immunoglobulin heavy or light chains, no surface Thy-1, and no surface Ia. In addition, they appeared to have little or no heavy chain gene rearrangements, including the D to J that occurs on both chromosomes prior to the VH rearrangement that forms the code for the C mu heavy chain polypeptide. Cells at even this early stage of differentiation could be induced by DC-T to express B220 on the surface and to synthesize and then to secrete immunoglobulins. These phenotypic changes were associated with a morphologic change in the cells to a lymphoblastoid appearance. Different patterns of immunoglobulin secretion resulted when pre-pre-B cells were cocultivated with DC-T from different tissues; SP DC-T induced the secretion of only IgM, PP DC-T induced the secretion of IgM as well as IgG and IgA. The early inductive event(s) appeared to occur during cell-cell contact in aggregates of the inducing DC-T and the pre-pre-B cells.
authors: Department of Microbiology, Birmingham Veterans Administration Hospital, Alabama.
authors: F
authors: F
authors: Saleh
authors: D M
authors: DM
authors: Spalding
authors: J A
authors: JA
authors: Griffin
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000944:Antigens, Differentiation, B-Lymphocyte; D001402:B-Lymphocytes / Q000145:classification / Q000276:immunology / Q000502:physiology*; D002454:Cell Differentiation; D002460:Cell Line; D003593:Cytoplasm / Q000276:immunology; D004247:DNA / Q000032:analysis; D015342:DNA Probes; D003713:Dendritic Cells / Q000276:immunology*; D007136:Immunoglobulins / Q000235:genetics; D007377:Interleukin-3 / Q000032:analysis; D017493:Leukocyte Common Antigens; D008099:Liver / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010641:Phenotype; D013154:Spleen / Q000032:analysis; D013234:Stem Cells / Q000145:classification / Q000276:immunology / Q000502:physiology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000944:Antigens, Differentiation, B-Lymphocyte; D015342:DNA Probes; D007136:Immunoglobulins; D007377:Interleukin-3; D004247:DNA; D017493:Leukocyte Common Antigens