Use of an antiserum against deglycosylated human mucins for cellular localization of their peptide precursors: antigenic similarities between bronchial and intestinal mucins.
Highly glycosylated regions of mucins, or glycopeptides, were obtained by proteolysis of human bronchial mucins. They were deglycosylated by treatment with a trifluoromethane sulfonic acid/anisole mixture and subsequent solvolysis with anhydrous liquid hydrogen fluoride. The resulting peptides were then used to raise an immune serum in rabbit. This immune serum was used to localize the peptide precursors of human respiratory mucins within bronchial cells, using an immunohistochemical method. Two main patterns of labeling were observed in the goblet cells: the entire cytoplasm of some goblet cells was immunoreactive, whereas in other cells the labeling was concentrated around the nucleus. In the respiratory mucous glands, the labeling was localized around or below the nucleus. The serous cells were not stained. Similar labeling was observed in human colon goblet cells. This immune serum seems to be specific for mucin-secreting cells and has a strong affinity for the perinuclear region of these cells.
Perini
J M
JM
Unité INSERM No. 16, Faculté de Médecine, Lille, France.
Marianne
T
T
Lafitte
J J
JJ
Lamblin
G
G
Roussel
P
P
Mazzuca
M
M
D000596:Amino Acids / Q000032:analysis; D000941:Antigens / Q000276:immunology*; D001980:Bronchi / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D000939:Epitopes / Q000276:immunology; D005947:Glucose / Q000378:metabolism; D006020:Glycopeptides / Q000032:analysis / Q000378:metabolism; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000032:analysis / Q000276:immunology; D009077:Mucins / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D009092:Mucous Membrane / Q000032:analysis / Q000276:immunology / Q000648:ultrastructure; D011498:Protein Precursors / Q000378:metabolism*
D000596:Amino Acids; D000941:Antigens; D000939:Epitopes; D006020:Glycopeptides; D007106:Immune Sera; D009077:Mucins; D011498:Protein Precursors; D005947:Glucose