Neuropeptides in interscapular and perirenal brown adipose tissue in the rat: a plurality of innervation.
The occurrence of neuropeptides in rat brown adipose tissue has been investigated. Immunohistochemical studies on interscapular and perirenal brown fat have demonstrated unequivocally the presence of substance-P (SP)-like, neuropeptide-Y (NPY)-like and calcitonin gene related-peptide (CGRP)-like immunoreactive elements (putative nerves) in adventitial distribution on inter- and intralobular supply arteries and in accompanying nerve bundles. At a more peripheral level, some NPY-like immunoreactive elements and a greater number of CGRP-like immunoreactive elements were observed in the parenchymal field. Somatostatin, bombesin, neurotensin, enkephalin, and vasoactive-intestinal-polypeptide immunoreactivities were not detected. No differences in neuropeptide distribution were noted between interscapular and perirenal brown fat. There is a degree of coincident distribution of SP, NPY and CGRP with that of noradrenergic nervous elements as visualized by condensation histochemistry. Since after 6-hydroxydopamine treatment not all the nerve terminals in rat brown adipose tissue are stigmatized (earlier report), the present results have been discussed in the light of a possible pluralism in innervation of brown adipose tissue.
Norman
D
D
Department of Anatomy, University College, Cardiff, UK.
Mukherjee
S
S
Symons
D
D
Jung
R T
RT
Lever
J D
JD
D002001:Adipose Tissue, Brown / Q000032:analysis*; D000818:Animals; D001341:Autonomic Nervous System / Q000032:analysis; D001839:Bombesin / Q000032:analysis; D015740:Calcitonin Gene-Related Peptide; D004745:Enkephalins / Q000032:analysis; D007150:Immunohistochemistry; D008297:Male; D009478:Neuropeptide Y / Q000032:analysis; D009479:Neuropeptides / Q000032:analysis*; D009496:Neurotensin / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D013373:Substance P / Q000032:analysis; D014660:Vasoactive Intestinal Peptide / Q000032:analysis
D004745:Enkephalins; D009478:Neuropeptide Y; D009479:Neuropeptides; D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D009496:Neurotensin; D015740:Calcitonin Gene-Related Peptide; D001839:Bombesin