Simultaneous sequencing of RNA by solid-phase chemical degradation using DE 81 anion-exchange paper.
A solid-phase chemical degradation method for simultaneous sequencing of RNA and RNA fragments has been developed using Whatman DE 81 anion-exchange paper as the support. The approach involves the following operations: (1) immobilization of the 3'-end labeled RNAs or RNA fragments on DE 81 paper; (2) washing; (3) modification reactions; (4) washing; (5) sorting of the paper segments; (6) aniline reaction; (7) lyophilization; (8) desorption of the RNA by TEAB; and (9) lyophilization.
Gross
B
B
Akademie der Wissenschaften der DDR.
Rosenthal
A
A
D000818:Animals; D001483:Base Sequence; D002852:Chromatography, Ion Exchange / Q000379:methods; D002854:Chromatography, Paper / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D007202:Indicators and Reagents; D008099:Liver / Q000032:analysis; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000235:genetics
D007202:Indicators and Reagents; D012335:RNA, Ribosomal; D012341:RNA, Ribosomal, 5S; D012313:RNA