Localization of epithelial markers and defence proteins in minor and major salivary glands.
Immunohistochemical demonstration of cytokeratin (CK), tissue polypeptide antigen (TPA), carcinoembryonic antigen (CEA), lactoferrin (Lf), lysozyme (Ly) and secretory component (SC) was performed in major salivary glands (MaSG), labial and palatal salivary glands (LSG, PSG). CK and TPA were demonstrated in ductal cells of all SGs. In addition, binding of anti-CK (Mw-56 Kd) antibody (AB) KL I was seen in all serious acini of MaSG, basophilic acini and demilunes of LSG and in some mucous acini of PSG. Binding of anti-CK (Mw 44-54 Kd) AB-PKK I, another polyclonal anti-CK (Mw 55-67 Kd) AB-PCK and anti-TPA AB was demonstrated in all basophilic acini of LSG, basophilic portions of mucous acini in PSG and not in acini of MaSG. Significant differences were observed in the expression of CEA, Ly, Lf and SC between acini of MaSG and MiSG which probably reflects different functions of these glands.
Vigneswaran
N
N
Department of Dermatology, University of Erlangen-Nürnberg.
Haneke
E
E
Hornstein
O P
OP
Niedermeier
W
W
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D007071:Immunoglobulin A, Secretory / Q000032:analysis*; D007633:Keratins / Q000032:analysis; D007781:Lactoferrin / Q000032:analysis; D009113:Muramidase / Q000032:analysis; D010455:Peptides / Q000032:analysis; D012469:Salivary Glands / Q000033:anatomy & histology*; D012470:Salivary Glands, Minor / Q000033:anatomy & histology*; D012471:Salivary Proteins and Peptides / Q000032:analysis*; D012635:Secretory Component / Q000032:analysis; D019396:Tissue Polypeptide Antigen
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D007071:Immunoglobulin A, Secretory; D010455:Peptides; D012471:Salivary Proteins and Peptides; D012635:Secretory Component; D019396:Tissue Polypeptide Antigen; D007633:Keratins; D009113:Muramidase; D007781:Lactoferrin