Loss of androgenic induction of alpha 2u-globulin gene family in the liver of NIH black rats.
Unlike all known strains of rat, the androgen-inducible alpha 2u-globulin gene family is totally silent in the liver of NIH black (NB) rats. No endocrinological or reproductive abnormalities are apparent, and the mRNA for the androgen-repressible hepatic protein SMP-2 is normally regulated in these animals. Furthermore, immunoblot analysis shows a normal level of the male-specific cytoplasmic androgen-binding protein. Cross-breeding of the NB male and Sprague-Dawley female shows that the hybrid male in the F-1 generation regains the androgen-dependent expression of alpha 2u-globulin in the liver. These results along with the observation of high constitutive level of alpha 2u-globulin mRNA in the preputial gland of NB rats indicate a tissue- and gene-specific regulatory defect which prevents androgenic induction of alpha 2u-globulin in the liver.
Chatterjee
B
B
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284.
Demyan
W F
WF
Song
C S
CS
Garg
B D
BD
Roy
A K
AK
D000510:Alpha-Globulins / Q000235:genetics*; D000727:Androgen-Binding Protein / Q000235:genetics; D000818:Animals; D015152:Blotting, Northern; D003600:Cytosol / Q000378:metabolism; D013196:Dihydrotestosterone / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation; D005796:Genes / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D005810:Multigene Family / Q000187:drug effects; D010052:Ovariectomy; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics* / Q000302:isolation & purification; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity
D000510:Alpha-Globulins; D000727:Androgen-Binding Protein; D013196:Dihydrotestosterone; D012313:RNA