Epitope mapping employing antibodies raised against short synthetic peptides: a study of the nicotinic acetylcholine receptor.
Antibodies were raised against eight synthetic peptides matching preselected portions of the amino acid sequence of nicotinic acetylcholine receptor (nAChR) from Torpedo marmorata. To increase the probability of obtaining antibodies specific for the exact sequence of the immunizing peptide, peptides of only five to seven amino acids in length were employed. Even under these limiting conditions some of the polyclonal rabbit immune sera showed cross-reactivity with other peptides and/or other sequence regions of the receptor. Further studies with polyclonal and monoclonal sera suggested that conformation and charge pattern rather than linear sequence are the essential determinants of antibody epitopes. Application of antibodies for topological studies therefore requires that the antibody specificity for a particular region of the antigen has been firmly established. Epitope mapping with the eight anti-peptide immune sera provides information on the accessibility to antibody of matching sequences within the receptor molecule. We find the sequence portions alpha 81-85, alpha 127-132, and alpha 190-195 to be freely accessible both at membrane-bound and at purified receptor. Binding of anti-alpha 387-392 serum does not prove accessibility of this region as the serum cross-reacts strongly with peptide fragments corresponding to the regions alpha 165-200 and beta 190-200 of nAChR from Torpedo californica. To permit binding of anti-alpha 137-142 immune serum, treatment of the receptor with endoglycosidase is required, showing that Asn-141 indeed is glycosylated in native nAChR. The homologous sequence of the other subunits differing only in one sequence position from alpha 137-142 is not accessible in native nAChR to antibody, indicating clear differences in folding of the receptor polypeptides. Sequence portions alpha 395-401 and alpha 161-166 must first be exposed by appropriate treatment to permit binding of respective serum. These results and previous epitope mapping studies by other laboratories are discussed with respect to the limited sequence specificity of antibodies.
Maelicke
A
A
Max-Planck-Institut für Ernährungsphysiologie, Dortmund, West Germany.
Plümer-Wilk
R
R
Fels
G
G
Spencer
S R
SR
Engelhard
M
M
Veltel
D
D
Conti-Tronconi
B M
BM
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D000937:Antigen-Antibody Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D007700:Kinetics; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases; D010455:Peptides / Q000138:chemical synthesis; D011487:Protein Conformation; D011978:Receptors, Nicotinic / Q000235:genetics* / Q000276:immunology; D014101:Torpedo
D000906:Antibodies; D000939:Epitopes; D046911:Macromolecular Substances; D010455:Peptides; D011978:Receptors, Nicotinic; D010447:Peptide Hydrolases