[Regulation of expression of the Escherichia coli htpR gene by antisense RNA].
A vector plasmid directing the synthesis of antisense RNA of Escherichia coli gene htpR has been constructed. For this fragment of htpR gene encoding the synthesis of N-terminal sequence of HtpR protein has been cloned in this plasmid in the opposite orientation under the control of Pr promoter of bacteriophage lambda. Under conditions of Pr transcription the induction the growth of bacterial cells carrying the recombinant plasmid was delayed and the rate of degradation of puromycin polypeptides was decreased 3-fold. Vectors constructed on the basis of such a principle may be used for thermoinducible synthesis of heterologous proteins with a simultaneous correction of the intracellular proteolysis.
Kiselev
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Tarasova
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D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D006360:Heat-Shock Proteins / Q000235:genetics*; D010957:Plasmids; D012313:RNA / Q000235:genetics*; D016372:RNA, Antisense
D006360:Heat-Shock Proteins; D016372:RNA, Antisense; D012313:RNA
Reguliatsiia ékspressii gena htpR Escherichia coli s pomoshch'iu antismyslovoĭ RNK.