Monoclonal antibodies against human alpha-fetoprotein. Exploitation of an unusual calcium-dependent interaction with the antigen for analytical and preparative purposes.
Seven murine hybridomas were established producing IgG1 monoclonal antibodies (mAbs) against three different epitopes of human alpha-fetoprotein (alpha FP). The interaction of two of these mAbs (AFP-01 and AFP-02) with the antigen was strongly inhibited by calcium-chelating agents. The mAb AFP-11 could be used for estimation of alpha FP by RIA in the concentration range of 5-100 ng/ml. Several combinations of the mAbs could be used in a two-site sandwich-ELISA for similar purposes. Immunoaffinity chromatography on a column of immobilized mAb AFP-01 permitted the purification of alpha FP.
Stefanová
I
I
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
Horejsí
V
V
Kristofová
H
H
Angelisová
P
P
Zizkovský
V
V
Hilgert
I
I
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D000937:Antigen-Antibody Reactions / Q000187:drug effects; D001667:Binding, Competitive; D002614:Chelating Agents / Q000494:pharmacology; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D007202:Indicators and Reagents; D051379:Mice; D008807:Mice, Inbred BALB C; D011863:Radioimmunoassay; D013329:Structure-Activity Relationship; D000509:alpha-Fetoproteins / Q000032:analysis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D002614:Chelating Agents; D000939:Epitopes; D007202:Indicators and Reagents; D000509:alpha-Fetoproteins; D003101:Collodion