Molecular aspects of age-related changes in albumin synthesis in female WAG/Rij rats.
The rate of albumin gene transcription and the level of albumin RNA sequences in liver nuclei of rats of various ages were determined to investigate the regulation of age-related changes in levels of cytoplasmic albumin mRNA in rat liver. No change was observed with age in both the rate of albumin gene transcription and the amount of nuclear albumin RNA sequences, which suggests that the increase in cytoplasmic albumin mRNA content in the rat liver with age is caused by a decreased turnover of this messenger. The length of poly(A)-tails of rat liver cytoplasmic RNA with age was also examined. Although no change with age was observed in the poly(A)-tail length sedimenting at the peak fraction, a shift towards shorter poly(A)-tails was found with age in the overall poly(A)-tail length distribution.
Horbach
G J
GJ
TNO Institute for Experimental Gerontology, Rijswijk, The Netherlands.
van der Boom
H
H
van Bezooijen
C F
CF
Yap
S H
SH
D000375:Aging; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D005260:Female; D005796:Genes; D008099:Liver / Q000254:growth & development* / Q000378:metabolism; D011061:Poly A / Q000032:analysis / Q000235:genetics; D012313:RNA / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000096:biosynthesis / Q000235:genetics*; D014158:Transcription, Genetic
D012333:RNA, Messenger; D012709:Serum Albumin; D011061:Poly A; D012313:RNA