Studies on non-specific interference due to serum in the avidin-biotin microELISA for monkey chorionic gonadotropin and a method for its elimination.
A study has been carried out on the non-specific interference due to serum in the avidin biotin microELISA for monkey chorionic gonadotropin. Results suggest that it is not due to any proteolytic activity in the serum, but immunoglobulin or associated factors interfering at the level of antigen-antibody interaction. This interference was eliminated by heating samples at 60 degrees C for 30 min.
Rao
A J
AJ
Department of Biochemistry, Indian Institute of Science, Bangalore.
Chakraborti
R
R
D000818:Animals; D001360:Avidin; D001710:Biotin; D001769:Blood; D006063:Chorionic Gonadotropin / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D008255:Macaca radiata; D008297:Male; D011480:Protease Inhibitors / Q000494:pharmacology; D005719:gamma-Globulins / Q000494:pharmacology
D006063:Chorionic Gonadotropin; D011480:Protease Inhibitors; D005719:gamma-Globulins; D001360:Avidin; D001710:Biotin