Evidence that an RGD-dependent receptor mediates the binding of oligodendrocytes to a novel ligand in a glial-derived matrix.
A simple adhesion assay was used to measure the interaction between rat oligodendrocytes and various substrata, including a matrix secreted by glial cells. Oligodendrocytes bound to surfaces coated with fibronectin, vitronectin and a protein component of the glial matrix. The binding of cells to all of these substrates was inhibited by a synthetic peptide (GRGDSP) modeled after the cell-binding domain of fibronectin. The component of the glial matrix responsible for the oligodendrocyte interaction is a protein which is either secreted by the glial cells or removed from serum by products of these cultures; serum alone does not promote adhesion to the same extent as the glial-derived matrix. The interaction of cells with this glial-derived matrix requires divalent cations and is not mediated by several known RGD-containing extracellular proteins, including fibronectin, vitronectin, thrombospondin, type I and type IV collagen, and tenascin.
Cardwell
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Department of Biological Chemistry, UCLA School of Medicine 90024.
Rome
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D000818:Animals; D001667:Binding, Competitive; D015153:Blotting, Western; D002448:Cell Adhesion; D002478:Cells, Cultured; D004492:Edetic Acid / Q000494:pharmacology; D005109:Extracellular Matrix / Q000502:physiology*; D005353:Fibronectins / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D008024:Ligands; D009457:Neuroglia / Q000166:cytology*; D009836:Oligodendroglia / Q000166:cytology*; D009842:Oligopeptides / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface / Q000502:physiology*; D011971:Receptors, Immunologic / Q000502:physiology*; D018000:Receptors, Peptide; D019096:Vitronectin
D005353:Fibronectins; D006023:Glycoproteins; D008024:Ligands; D009842:Oligopeptides; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D018000:Receptors, Peptide; D019096:Vitronectin; C051382:arginyl-glycyl-aspartic acid directed cell adhesion receptor; D004492:Edetic Acid