Separation of the complex asparagine-linked oligosaccharides of the glycoprotein fetuin and elucidation of three triantennary structures having sialic acids linked only to galactose residues.
Asparagine-linked oligosaccharides of the glycoprotein fetuin were isolated as reducing oligosaccharides after hydrazinolysis/re-N-acetylation/mild acid treatment of the Pronase-digested glycoprotein. The sialylated oligosaccharides were separated by high-performance liquid chromatography in two different systems, which resulted in greater than 35 fractions, comprising di-, tri-, tetra-, and pentasialylated oligosaccharides. The major components were isomeric structures comprising the tri- and tetrasialylated fractions. In this and the accompanying paper (Cumming et al., 1989), the structures of 10 of the major components of the tri-, tetra-, and pentasialylated oligosaccharide fractions are described. Separation protocols and three isolated structures having sialic acid linked only to galactose are presented in this paper.
Bendiak
B
B
Department of Biochemistry, Hospital for Sick Children, Toronto, Ontario, Canada.
Harris-Brandts
M
M
Michnick
S W
SW
Carver
J P
JP
Cumming
D A
DA
D001216:Asparagine; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D005690:Galactose / Q000032:analysis*; D006020:Glycopeptides / Q000302:isolation & purification; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000302:isolation & purification*; D012794:Sialic Acids / Q000032:analysis*; D000509:alpha-Fetoproteins
D006020:Glycopeptides; D007202:Indicators and Reagents; D009844:Oligosaccharides; D012794:Sialic Acids; D000509:alpha-Fetoproteins; D001216:Asparagine; D005690:Galactose