Decrease of oligo-2',5'-adenylate synthetase activity in BALB3T3 cell persistently infected with Moloney murine leukemia virus.
The induction of oligo-2',5'-adenylate synthetase (2-5AS) activity by interferon (IFN) was decreased in BALB3T3 cells persistently infected with Moloney murine leukemia virus (Mo-MLV) as compared with uninfected cells. Furthermore, the correlation between increased susceptibility to vesicular stomatitis virus (VSV) infection and reduced 2-5AS activity was recognized in the Mo-MLV persistently infected cells. The decrease of enzyme activity was confirmed by a solid phase reaction and an analysis of reaction products by Fast Polynucleotide Liquid Chromatography (FPLC) in addition to a liquid phase reaction. In a solid-phase reaction, the enzyme protein binds to polyinosinate-cytidylate (Poly I:C) agarose beads and other cellular proteins can be washed out from the reaction mixtures. Therefore, these results indicate that the decrease of IFN-induced enzyme activity is due to the suppression of transcription and/or translation of 2-5AS mRNA. A decreased amount of 2-5AS mRNA in persistently infected cells was observed by Northern blot and dot-blot hybridization. On the other hand, cell lysate of Mo-MLV infected cells inhibited the 2-5AS activity in liquid phase reaction. The inhibition may also be partly due to the degradation of oligo-2',5'-adenylate (2-5A) formed by 2-5AS.
Fujii
N
N
Department of Microbiology, Sapporo Medical College, Japan.
Oguma
K
K
Yamashita
T
T
Fujinaga
K
K
Kakinuma
M
M
Yashiki
T
T
D015088:2',5'-Oligoadenylate Synthetase / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D000818:Animals; D002457:Cell Extracts / Q000494:pharmacology; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003593:Cytoplasm; D007158:Immunologic Techniques; D007372:Interferons / Q000494:pharmacology; D007942:Leukemia, Experimental / Q000201:enzymology*; D008979:Moloney murine leukemia virus; D009693:Nucleic Acid Hybridization; D012334:RNA, Neoplasm / Q000302:isolation & purification
D002457:Cell Extracts; D012334:RNA, Neoplasm; D007372:Interferons; D015088:2',5'-Oligoadenylate Synthetase