Mapping of a DNA sequence that down-regulates the expression of an interferon-induced gene.
Recently, an interferon response element (IRE) of approximately 24 bp has been identified by Reich et al. (7) that allows certain genes to be actively transcribed by type I interferons (IFNs). The transcription of several IFN-induced genes in some cells (human fibroblasts and melanoma cells) is inhibited by long-term exposure to IFN. This process requires the synthesis of a new protein(s). Evidence is presented that this same IRE contains the information necessary for long-term IFN treatment to down-regulate the transcription of those genes whose expression it initially activates.
Akai
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Laboratory of Pathology, National Cancer Institute, Bethesda, MD 20892.
Larner
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D003513:Cycloheximide / Q000494:pharmacology; D005347:Fibroblasts; D005786:Gene Expression Regulation; D006564:Herpesviridae / Q000235:genetics; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology; D007372:Interferons / Q000494:pharmacology*; D008545:Melanoma; D012313:RNA / Q000235:genetics; D015347:RNA Probes; D011994:Recombinant Proteins; D012045:Regulatory Sequences, Nucleic Acid; D013937:Thymidine Kinase / Q000235:genetics; D014162:Transfection; D014407:Tumor Cells, Cultured
D007370:Interferon Type I; D015347:RNA Probes; D011994:Recombinant Proteins; D012313:RNA; D007372:Interferons; D003513:Cycloheximide; D013937:Thymidine Kinase