[Antisense RNA p53 inhibits proliferation of normal and transformed cells].
p53 anti-sense sequences were introduced into normal NIH3T3 and transformed CMS 4 cells by infection with the recombinant retrovirus carrying a repeat of the 5'-terminal fragment of p53 cDNA. Clones selected for G418 resistance showed a marked inhibition of proliferative capacity and a reduced ability to enter DNA replication after stimulation of quiescent cells with serum. Clones showing moderate inhibition of proliferation were shown to contain truncated anti-sense DNA integrated into the genome. The anti-sense DNA was transcribed and it correlated with the reduction of the p53 protein level in the cell clones studied. We conclude that the appropriate expression of p53 appears to be required for cell proliferation.
Prasolov
V S
VS
Chumakov
P M
PM
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002461:Cell Line, Transformed; D004247:DNA / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D051379:Mice; D009363:Neoplasm Proteins / Q000235:genetics*; D010750:Phosphoproteins / Q000235:genetics*; D012313:RNA / Q000235:genetics*; D016372:RNA, Antisense; D011995:Recombination, Genetic; D012190:Retroviridae / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection; D016159:Tumor Suppressor Protein p53
D004279:DNA, Viral; D009363:Neoplasm Proteins; D010750:Phosphoproteins; D016372:RNA, Antisense; D016159:Tumor Suppressor Protein p53; D012313:RNA; D004247:DNA
Antismyslovaia RNK p53 podavliaet proliferatsiiu normal'nykh i transformirovannykh kletok.