title: The induction of respiratory allergy in guinea-pigs following intradermal injection of trimellitic anhydride: a comparison with the response to 2,4-dinitrochlorobenzene.
abstract: Guinea-pigs injected intradermally with the known respiratory sensitiser trimellitic anhydride (TMA) developed high-titre antigen-specific homocytotropic (IgG1 and IgE) antibodies. Many of the sensitised animals responded to a challenge by inhalation with either free TMA or a TMA-protein conjugate with a change in respiratory rate, reflecting the onset of bronchoconstriction. Guinea-pigs were also injected intradermally with 2,4-dinitrochlorobenzene (DNCB), which is a potent skin sensitiser in man but which has not been reported to cause respiratory allergy. These animals developed only low-titre homocytotropic antibodies and were unresponsive to an inhalation challenge with either free or conjugated hapten. The animals were, however, contact-sensitised to the chemical.
authors: ICI Central Toxicology Laboratory, Nr Macclesfield, Cheshire, U.K.
authors: P A
authors: PA
authors: Botham
authors: N J
authors: NJ
authors: Rattray
authors: D R
authors: DR
authors: Woodcock
authors: S T
authors: ST
authors: Walsh
authors: P M
authors: PM
authors: Hext
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D000917:Antibody Formation; D004137:Dinitrochlorobenzene; D000939:Epitopes; D005260:Female; D006168:Guinea Pigs; D006241:Haptens / Q000008:administration & dosage; D007114:Immunization; D007073:Immunoglobulin E / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D007271:Injections, Intradermal; D010795:Phthalic Acids / Q000633:toxicity*; D010796:Phthalic Anhydrides / Q000008:administration & dosage / Q000633:toxicity*; D012130:Respiratory Hypersensitivity / Q000139:chemically induced* / Q000276:immunology
chemical_list: D004137:Dinitrochlorobenzene; D000939:Epitopes; D006241:Haptens; D007074:Immunoglobulin G; D010795:Phthalic Acids; D010796:Phthalic Anhydrides; D007073:Immunoglobulin E; C015559:trimellitic anhydride