Four cytotoxic human-human hybridoma antibodies that react with HLA-B27.
PBMC were isolated from a multiparous woman with HLA-B27 specific Abs in her serum. The HLA type of the donor was A2,9:B7. The PBMC were EBV transformed, and four cell lines making cytotoxic Abs to HLA-B27+ cells prepared. Hybridomas were constructed by fusing the EBV lines with the human fusion partner KR4. All four mAbs were of IgM isotype. One mAb (TrBH12) reacted specifically with B27+, B37+ and Bw47+ lymphoblastoid cell lines and with all B27+ PBMC except for a rare variant so far found only in one Norwegian family. Another mAb (Tr3B6) was cytotoxic for all B27+ cells tested, including the TrBH12- variant; in addition, it showed weaker cross-reactions to Bw42, B49 and a cell line with the probable phenotype B7,38. Supernatant from the Tr3B6 hybridoma was tested in lymphocytotoxicity against a panel of 658 individuals, 141 of whom were B27+. With this panel, Tr3B6 showed perfect correlation with HLA-B27. The two last mAbs (TrCG10 and TrBF1) reacted with all B27+ cells tested, but in addition showed quite extensive cross-reactions.
Hansen
T
T
Institute of Medical Biology, University of Tromsø School of Medicine, Norway.
Bratlie
A
A
Hannestad
K
K
D000911:Antibodies, Monoclonal / Q000276:immunology*; D002461:Cell Line, Transformed; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D015235:HLA-B Antigens / Q000276:immunology*; D015796:HLA-B27 Antigen; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D015235:HLA-B Antigens; D015796:HLA-B27 Antigen; C106993:HLA-B47 antigen