title: Monoclonal antibodies to P2 basic protein and their use in the investigation of some aspects of the Guillain-Barré syndrome.
abstract: The production and characterization of two mouse monoclonal antibodies (MAbs) raised against human basic protein P2 is described. The two MAbs recognise two non-overlapping epitopes on P2. Using these two MAbs as a probe, a search was made for the presence of anti-idiotype (Ab2) antibodies directed against anti-P2 (Ab1) autoantibodies in sera of patients with Guillain-Barré syndrome (GBS). In sera of twenty GBS patients no such Ab2 antibodies could be demonstrated. Epitopes present on cytomegalo virus- or Epstein-Barr virus-infected cells, which may play a part in triggering GBS, were not recognised by the two MAbs.
authors: Department of Neurology, University Hospital, Utrecht, The Netherlands.
authors: J A
authors: JA
authors: Luijten
authors: S D
authors: SD
authors: Reijneveld-de Jong
authors: T
authors: T
authors: Teerlink
authors: V T
authors: VT
authors: Camps
authors: A D
authors: AD
authors: Osterhaus
authors: A G
authors: AG
authors: UytdeHaag
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001323:Autoantibodies / Q000032:analysis; D015415:Biomarkers / Q000097:blood*; D002420:Cauda Equina / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D004676:Myelin Basic Protein / Q000032:analysis* / Q000276:immunology; D018996:Myelin P2 Protein; D011129:Polyradiculoneuropathy / Q000276:immunology* / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D015415:Biomarkers; D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D004676:Myelin Basic Protein; D018996:Myelin P2 Protein