Differential release of coexisting neurotransmitters: frequency dependence of the efflux of substance P, thyrotropin releasing hormone and [3H]serotonin from tissue slices of rat ventral spinal cord.
In few systems has the release of coexisting classical and peptide neurotransmitters been studied. Here the release of substance P-like immunoreactivity (SP-LI), thyrotropin releasing hormone-like immunoreactivity (TRH-LI) and [3H]serotonin ([3H]5-HT) from tissue slices of rat ventral spinal cord was investigated in a superfusion system. The slices were stimulated electrically with field stimulation (900 pulses, 2 ms duration, 36 V) at frequencies between 0.25 Hz and 40 Hz. The evoked fractional release of SP-LI increased significantly from 0.46 to 1.24% of the total tissue store when the frequency of stimulation was changed from 3 to 10 Hz, while the evoked fractional release of TRH-LI increased significantly from 0.28 to 0.71% of the total tissue store with increasing frequency of stimulation between 0.5 and 3 Hz. The evoked fractional release of [3H]5-HT did not show any significant change when the frequency of stimulation was changed in the frequency range of 0.25-40 Hz but remained between 5.6 and 7.2% of the total tissue store. It appears that at frequencies lower than 0.5-1 Hz these 5-HT/SP/TRH neurons may function predominantly as serotonergic neurons. At 3 Hz stimulation with 900 pulses the extracellular Ca2+ concentration required for half-maximal release of [3H]5-HT was 1.2 mmol l-1, while for half-maximal release of SP-LI significantly higher concentrations of Ca2+ (4.2 mmol l-1) would be required.(ABSTRACT TRUNCATED AT 250 WORDS)
Iverfeldt
K
K
Department of Biochemistry, Arrhenius Laboratory, University of Stockholm, Sweden.
Serfözö
P
P
Diaz Arnesto
L
L
Bartfai
T
T
D000818:Animals; D002118:Calcium / Q000502:physiology; D004558:Electric Stimulation; D051381:Rats; D013116:Spinal Cord / Q000378:metabolism*; D013373:Substance P / Q000378:metabolism*; D013973:Thyrotropin-Releasing Hormone / Q000378:metabolism*
D013373:Substance P; D013973:Thyrotropin-Releasing Hormone; D002118:Calcium