Change in hepatic gene expression after shock/resuscitation.
In response to specific stresses, such as a heat pulse or the sequence of hypoxia-reoxygenation, each isolated cell (prokaryotic and eukaryotic) that has been studied characteristically alters gene expression to synthesize a set of proteins (heat-shock proteins) that are important for intracellular homeostasis. To determine whether a corresponding response occurs within parenchymal cells in vivo when subjected to the complex stress of circulatory shock, liver biopsy specimens were obtained from a swine model of cardiogenic shock before and after shock/resuscitation. With use of complementary DNA prepared from post-shock/resuscitation messenger RNA, a library was constructed and screened for differential gene expression. Of 32/4000 clones initially screened as positive for induction after shock/resuscitation, six were confirmed positive by Northern blot analysis. The nucleotide sequences of two of these six have been determined, and one has been unambiguously identified as metallothionein.
Buchman
T G
TG
Department of Surgery, Johns Hopkins Medical Institutions, Baltimore, Md.
Cabin
D E
DE
Porter
J M
JM
Bulkley
G B
GB
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D004247:DNA / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005260:Female; D005786:Gene Expression Regulation; D008099:Liver / Q000378:metabolism / Q000502:physiology*; D008668:Metallothionein / Q000235:genetics; D008969:Molecular Sequence Data; D012313:RNA / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012151:Resuscitation; D012769:Shock / Q000235:genetics* / Q000628:therapy; D013552:Swine
D012333:RNA, Messenger; D012313:RNA; D004247:DNA; D008668:Metallothionein