Transcriptional regulation of a Xenopus embryonic epidermal keratin gene.
XK81A1 is a type I epidermal keratin gene expressed in early developmental stages of Xenopus (Jonas et al. 1985). Fusion of the keratin promoter (-5900 to +26) to a human beta globin gene led to fully epidermis-specific accumulation of human globin mRNA and protein when this DNA was injected into fertilized eggs. Further localization of regulatory sequences was accomplished by injecting marked, 5'-deleted keratin gene DNA into fertilized eggs and evaluating tissue specificity of expression. All 5' flanking DNA upstream from -487 could be removed without interfering with keratin gene expression or regulation. These results suggest that the primary mode of regulation of epidermis-specific keratin gene expression is at the level of transcription, and that sequence elements in the 5' flanking region of the keratin gene, between -487 and +26, are responsible for this regulation.
Jonas
E A
EA
Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, Bethesda, MD 20892.
Snape
A M
AM
Sargent
T D
TD
D000818:Animals; D001483:Base Sequence; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D004274:DNA, Recombinant; D004817:Epidermis / Q000196:embryology* / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D005914:Globins / Q000235:genetics; D007633:Keratins / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic; D014162:Transfection; D014982:Xenopus laevis / Q000196:embryology
D004274:DNA, Recombinant; D012333:RNA, Messenger; D007633:Keratins; D005914:Globins