[CA 19-9 like epitope(s) on carcinoembryonic antigen molecules].
CA 19-9 epitope(s) on carcinoembryonic antigen (CEA) was studied by a method designated the hybrid immunoradiometric-assay (hybrid IRMA) using solid phase CEA antibody to capture the CEA molecule and radio-labeled NS 19-9 antibody as a detector. The radioactivities of the hybrid IRMA of respective sera with certain cancers differed from that of normal sera. The specific radioactivities of hybrid IRMA did not disappear on washing with a reaction mixture containing Triton X-100 as a detergent. Treatment of the sample with neuraminidase or periodate diminished the specific bound radioactivities, similar to CA 19-9 antigen. Furthermore, the treatment of CEAs by PI-PLC had no effect on the radioactivities. Taken together, it was suggested that CEA sugar moieties contain CA 19-9 like epitope(s).
Nagata
A
A
Department of Biochemistry, Saitama Medical School.
Komoda
T
T
D015295:Antigens, Tumor-Associated, Carbohydrate / Q000276:immunology*; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D000939:Epitopes / Q000032:analysis*; D006801:Humans; D015592:Immunoradiometric Assay / Q000379:methods; D066298:In Vitro Techniques; D009369:Neoplasms / Q000276:immunology*; D009439:Neuraminidase; D010504:Periodic Acid; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases
D015295:Antigens, Tumor-Associated, Carbohydrate; D002272:Carcinoembryonic Antigen; D000939:Epitopes; D010504:Periodic Acid; C009288:metaperiodate; D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D009439:Neuraminidase; D043265:Phosphatidylinositol Diacylglycerol-Lyase