Identification of a functional promoter for the Escherichia coli gdhA gene and its regulation.
Glutamate dehydrogenase (GDH) catalyzes the synthesis of L-glutamate from 2-oxoglutarate and ammonia. The complete nucleotide sequence of the Escherichia coli gdhA gene, as well as its 5' and 3' flanking regions have been previously reported [Valle et al., Gene 23 (1983) 199-209; 27 (1984) 193-199]. In this paper we present data on the GDH specific activities using both excess and limiting concentrations of ammonia as nitrogen sources. Evidence is presented on the regulation of the mRNA levels for this enzyme by the ammonia concentration in the growth medium. We have identified a single and apparently invariant transcript for several metabolic growth conditions. We also report the identification of a functional promoter and the corresponding transcription start point under several growth conditions. Finally, possible regulatory sequences located at the 5' flanking region of the gdhA gene are discussed.
Riba
L
L
Departamento de Biología Molecular, Universidad Nacional Autónoma de México, Morelos.
Becerril
B
B
Servín-González
L
L
Valle
F
F
Bolivar
F
F
D001483:Base Sequence; D015152:Blotting, Northern; D002872:Chromosome Deletion; D003001:Cloning, Molecular; D003470:Culture Media; D004262:DNA Restriction Enzymes; D004720:Endonucleases; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005969:Glutamate Dehydrogenase / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012313:RNA / Q000302:isolation & purification; D015183:Restriction Mapping; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013753:Tetracycline Resistance; D014158:Transcription, Genetic
D003470:Culture Media; D009838:Oligodeoxyribonucleotides; D012313:RNA; D005969:Glutamate Dehydrogenase; D004720:Endonucleases; D004262:DNA Restriction Enzymes; D015719:Single-Strand Specific DNA and RNA Endonucleases