Immunohistochemical localization of transport mediators in Wilms' tumor: comparison with fetal and mature human kidney.
Immunostaining for Na+, K+-ATPase, carbonic anhydrase (CA) II, and band 3 anion channel glycoprotein was compared in developing and mature human kidneys and in Wilms' tumors. In fetal kidneys, ATPase first appeared in proximal and distal tubules. At birth an adult pattern was present with abundant enzyme in all segments of the distal tubule and lesser amounts in proximal and collecting tubules. CA II was detected in fetal kidneys first in proximal and then in distal tubules and eventually, as in the adult, throughout the nephron. Band 3 glycoprotein was not detected in fetal kidneys and only weak staining was present in the basolateral plasmalemma of intercalated cells in newborn and infant kidneys. The number of cells reactive for band 3 and the intensity of staining in a given cell increased to near adult levels at about 2 years. This finding may provide a partial explanation for the 'physiological acidosis' characterized by a low systemic pH in newborn and young infants. ATPase was present in basolateral membranes of most epithelial cells in nonanaplastic Wilms' tumors but was absent in the epithelial component of two anaplastic Wilms' tumors. CA II was detected only in a few epithelial cells in four tumors. Neoplastic epithelial cells reactive for CA II also stained for ATPase but not vice versa. Band 3 glycoprotein was not detected in any Wilms' tumor. These findings show that the immunohistochemical assessment of protein involved in electrolyte transport provides a further means for determining the relative level of differentiation of tumor cells of epithelial origin and suggest that these methods may be a valuable aid in determining the prognosis of some carcinomas.
Hennigar
R A
RA
Department of Pathology, Johns Hopkins Hospital, Baltimore, Maryland.
Garvin
A J
AJ
Hazen-Martin
D J
DJ
Schulte
B A
BA
D000293:Adolescent; D000328:Adult; D001457:Anion Exchange Protein 1, Erythrocyte / Q000032:analysis; D001692:Biological Transport; D002256:Carbonic Anhydrases / Q000032:analysis; D004573:Electrolytes / Q000378:metabolism*; D005333:Fetus / Q000032:analysis; D005865:Gestational Age; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D007231:Infant, Newborn; D007473:Ion Channels / Q000032:analysis; D007668:Kidney / Q000032:analysis* / Q000196:embryology; D007680:Kidney Neoplasms / Q000032:analysis*; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis; D009396:Wilms Tumor / Q000032:analysis*
D001457:Anion Exchange Protein 1, Erythrocyte; D004573:Electrolytes; D007473:Ion Channels; D002256:Carbonic Anhydrases; D000254:Sodium-Potassium-Exchanging ATPase