Analysis of platelet glycoproteins in thrombocytopathias using the lectin-avidin-biotin-peroxidase (LABP) technique.
The application of the lectin-avidin-biotin-peroxidase (LABP) technique for detecting platelet glycoprotein abnormalities in thrombocytopathias is described. Platelet proteins from patients with Glanzmann's thrombasthenia or Bernard-Soulier syndrome were separated by two-dimensional O'Farrell gel electrophoresis, stained with silver or electroblotted onto nitrocellulose sheets. Nitro-cellulose blots were stained utilizing the LABP technique. The absence or severe reduction of glyco-proteins IIb and IIIa and fibrinogen in the platelet protein pattern of patients with thrombasthenia as well as the absence or marked reduction of glycoproteins Ib and V in the platelet protein pattern of a patient with Bernard-Soulier syndrome were clearly demonstrated.
Kehrel
B
B
Institute for Arteriosclerosis Research, University of Münster, FRG.
Kokott
R
R
Stenzinger
W
W
Balleisen
L
L
D001606:Bernard-Soulier Syndrome / Q000097:blood*; D001791:Blood Platelet Disorders / Q000097:blood*; D001792:Blood Platelets / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D010980:Platelet Membrane Glycoproteins / Q000097:blood*; D013915:Thrombasthenia / Q000097:blood*
D010980:Platelet Membrane Glycoproteins