[Monoclonal antibodies, interacting with antigenic determinants of the human immunodeficiency virus].
Twenty-five hybridomas of CMK series were generated which produced monoclonal antibodies (MCA) to human immunodeficiency virus (HIV). The MCA were shown to react with HIV antigenic determinants in enzyme-immunoassays to titres 5 X 10(3)--10(5). It was established by immunoblot that some MCA interact with HIV proteins having molecular weights of 60-80 KD, and other MCA with proteins of low molecular weight (9-17 KD). Using recombinant proteins--products of env and gag genes, the immunoblot showed 5 MCA to be specific for proteins of the env gene and 5 others for proteins of the gag gene. Comparative enzyme immunoassays of interaction of MCA of the CMK series with viruses of infectious anemia of horses and HIV led to the conclusion that 4 MCA recognize common determinants of the lentiviruses under study whereas 5 other MCA react specifically with HIV alone.
Shumaĭ
E P
EP
Kushch
A A
AA
Bukrinskaia
A G
AG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D015686:Gene Products, env / Q000276:immunology; D015683:Gene Products, gag / Q000276:immunology; D006678:HIV / Q000276:immunology*; D015488:HIV Antigens / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D000939:Epitopes; D015686:Gene Products, env; D015683:Gene Products, gag; D015488:HIV Antigens
Monoklonal'nye antitela, vzaimodeĭstvuiushchie s antigennymi determinantami virusa immunodefitsita cheloveka.