Double-stranded ribonuclease coinduced with interferon.
Double-stranded (ds) RNA and many viruses are inducers of interferon (IFN), the latter presumably because they contain, or can form, dsRNA. Concomitant with the induction of IFN in chicken embryo cells was the induction of a novel double-stranded ribonuclease (dsRNase), which was released into the medium and continued to accumulate long after IFN production ceased. Only avian cells (chicken, quail, turkey, or duck) expressed high levels of this dsRNase; mammalian, turtle, or fish cells did not. Production of the nuclease was inducer dose-dependent. Optimum pH and cation requirements distinguished it from other dsRNase activities. Degradation of dsRNA was endonucleolytic. Activity resided in a molecule of an Mr of approximately 34,500. Low levels of a single-stranded (ss) RNase activity were inseparable from the dsRNase. The role for a dsRNA-inducible dsRNase released from cells is unknown.
Meegan
J M
JM
Department of Molecular and Cell Biology, The University of Connecticut, Storrs 06269.
Marcus
P I
PI
D000818:Animals; D001717:Birds / Q000196:embryology / Q000378:metabolism*; D002412:Cations; D002642:Chick Embryo; D004372:Ducks / Q000196:embryology; D004722:Endoribonucleases / Q000096:biosynthesis*; D004790:Enzyme Induction; D006863:Hydrogen-Ion Concentration; D007369:Interferon Inducers / Q000494:pharmacology; D007372:Interferons / Q000378:metabolism*; D007700:Kinetics; D009522:Newcastle disease virus / Q000502:physiology / Q000528:radiation effects; D011070:Poly I-C / Q000494:pharmacology; D011784:Quail / Q000196:embryology; D012330:RNA, Double-Stranded / Q000378:metabolism; D013045:Species Specificity; D013379:Substrate Specificity; D014422:Turkeys / Q000196:embryology; D014466:Ultraviolet Rays
D002412:Cations; D007369:Interferon Inducers; D012330:RNA, Double-Stranded; D007372:Interferons; D004722:Endoribonucleases; C044803:ds RNase; D011070:Poly I-C