Nitric oxide from L-arginine stimulates the soluble guanylate cyclase in adrenal glands.
The formation of nitric oxide (NO) by an L-arginine:NO synthase and its stimulation of the soluble guanylate cyclase was studied in rat whole adrenal and bovine cortex and medulla cytosol. In the presence of L-arginine, the stimulation of soluble guanylate cyclase was accompanied by the formation of citrulline and NO2-, formed from NO. The NO synthase was NADPH- and Ca(2+)-dependent and was inhibited by several L-arginine analogues. These results indicate that rat and bovine adrenal cytosol contains an L-arginine:NO synthase.
Palacios
M
M
Wellcome Research Laboratories, Beckenham, Kent, U.K.
Knowles
R G
RG
Palmer
R M
RM
Moncada
S
S
D000302:Adrenal Cortex / Q000201:enzymology; D000311:Adrenal Glands / Q000201:enzymology*; D000313:Adrenal Medulla / Q000201:enzymology; D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D000818:Animals; D001120:Arginine / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology; D002417:Cattle; D006152:Cyclic GMP / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology; D006162:Guanylate Cyclase / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D009249:NADP / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase; D051381:Rats; D011919:Rats, Inbred Strains
D009569:Nitric Oxide; D009249:NADP; D001120:Arginine; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D006162:Guanylate Cyclase; D006152:Cyclic GMP; D002118:Calcium