Western blot analysis of Amblyomma americanum-derived stage-specific and shared antigens using serum from guinea pigs expressing resistance.
Serum from guinea pigs expressing resistance to larval, nymphal and adult Amblyomma americanum ticks was used in Western blot analyses to identify potential antigens from egg, larval and nymphal, and female salivary gland extract preparations. The results demonstrate multiple antigens unique to each life stage, as well as several shared proteins between the three life stages. However, it appears as if two particular proteins of 25 and 38 kDa may be more important than others, based upon their prevalence and intensity of recognition in this assay relative to other polypeptides.
Brown
S J
SJ
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana 61801.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D005260:Female; D006168:Guinea Pigs; D007106:Immune Sera / Q000276:immunology; D007118:Immunoassay; D007814:Larva / Q000276:immunology; D009758:Nymph / Q000276:immunology; D010063:Ovum / Q000276:immunology; D012469:Salivary Glands / Q000276:immunology; D013987:Ticks / Q000276:immunology*
D000941:Antigens; D000939:Epitopes; D007106:Immune Sera