Monoclonal antibodies to P2 basic protein and their use in the investigation of some aspects of the Guillain-Barré syndrome.
The production and characterization of two mouse monoclonal antibodies (MAbs) raised against human basic protein P2 is described. The two MAbs recognise two non-overlapping epitopes on P2. Using these two MAbs as a probe, a search was made for the presence of anti-idiotype (Ab2) antibodies directed against anti-P2 (Ab1) autoantibodies in sera of patients with Guillain-Barré syndrome (GBS). In sera of twenty GBS patients no such Ab2 antibodies could be demonstrated. Epitopes present on cytomegalo virus- or Epstein-Barr virus-infected cells, which may play a part in triggering GBS, were not recognised by the two MAbs.
Luijten
J A
JA
Department of Neurology, University Hospital, Utrecht, The Netherlands.
Reijneveld-de Jong
S D
SD
Teerlink
T
T
Camps
V T
VT
Osterhaus
A D
AD
UytdeHaag
A G
AG
D000818:Animals; D000911:Antibodies, Monoclonal; D001323:Autoantibodies / Q000032:analysis; D015415:Biomarkers / Q000097:blood*; D002420:Cauda Equina / Q000473:pathology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D004676:Myelin Basic Protein / Q000032:analysis* / Q000276:immunology; D018996:Myelin P2 Protein; D011129:Polyradiculoneuropathy / Q000276:immunology* / Q000473:pathology
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D015415:Biomarkers; D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D004676:Myelin Basic Protein; D018996:Myelin P2 Protein