[Immunoenzymatic detection of specific brain antigens as a criterion of the permeation of blood-brain barrier in rats with acute cerebral ischemia].
EIA detection system for the measurement of alpha 2 M globulin and GFAP antigen has been developed. The limit of the sensibility was only 1 ng/ml for alpha 2M and 0.8 ng/ml for GFAP. The system was used for the studies of the penetration through the blood-brain barrier in rats with experimental acute brain ischemia. The measurement of alpha 2M and GFAP antigens by EIA technique 16-20 hours after the occlusion of the carotid artery has revealed disturbances in the blood-brain barrier permeability for specific brain proteins. The method is recommended for indirect evaluation of the blood-brain barrier functional disorders.
Chekhonin
V P
VP
Morozov
G V
GV
Markovkin
V M
VM
Blizniukova
A S
AS
D000818:Animals; D000941:Antigens / Q000032:analysis*; D001812:Blood-Brain Barrier; D001921:Brain / Q000276:immunology*; D002545:Brain Ischemia / Q000276:immunology / Q000503:physiopathology*; D000939:Epitopes; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis*; D007124:Immunoenzyme Techniques; D008297:Male; D051381:Rats; D000511:alpha-Macroglobulins / Q000032:analysis*
D000941:Antigens; D000939:Epitopes; D005904:Glial Fibrillary Acidic Protein; D000511:alpha-Macroglobulins
Immunofermentativnaia detektsiia spetsificheskikh antigenov mozga kak kriteriĭ pronitsaemosti gematoéntsefalicheskogo bar'era krys pri ostroĭ ishemii golovnogo mozga.