A modified Brown and Hopps stain for identification of gram-positive and gram-negative microorganisms in glycol methacrylate-embedded tissues.
Plastic embedding is a recently developed technique that has been shown to be superior to conventional paraffin embedding in the histopathologic identification of microorganisms in tissue specimens. This report describes a modification of the Brown and Hopps stain for the differentiation of gram-positive and gram-negative microorganisms in 3-micron-thick plastic sections. This technique is easily performed and has a rapid turnaround time. This staining method permits a more precise histopathologic diagnosis of infectious agents than is possible in paraffin-embedded tissues.
Schwartz
D A
DA
Department of Pathology and Laboratory Medicine, Hahnemann University School of Medicine, Philadelphia.
Munger
R
R
Lazzaro
B
B
Lumb
G
G
D000179:Acrylates; D000818:Animals; D005658:Fungi / Q000145:classification / Q000302:isolation & purification; D006090:Gram-Negative Bacteria / Q000145:classification / Q000302:isolation & purification*; D006094:Gram-Positive Bacteria / Q000145:classification / Q000302:isolation & purification*; D006652:Histological Techniques; D006801:Humans; D008689:Methacrylates; D051381:Rats; D013194:Staining and Labeling / Q000379:methods*
D000179:Acrylates; D008689:Methacrylates; C005044:hydroxyethyl methacrylate