Characterisation of the reactivity of autoantibodies in primary biliary cirrhosis.
Autoantibodies in the sera of patients with primary biliary cirrhosis, shown previously to recognise the E2 polypeptide of the mammalian pyruvate dehydrogenase complex (PDC), have been demonstrated to react with the E2 component of PDC from bacteria (E. coli) and yeast (S. cerevisiae). Limited tryptic digestion, which cleaves E2 into well-characterised domains, followed by Western blotting indicates that the main immunodominant region of PDC E2 lies within the lipoic acid-containing domains of the polypeptide.
Fussey
S P
SP
Department of Biochemistry and Genetics Medical School, University of Newcastle upon Tyne, England.
Bassendine
M F
MF
James
O F
OF
Yeaman
S J
SJ
D000107:Acetylation; D000941:Antigens / Q000276:immunology; D001323:Autoantibodies / Q000276:immunology*; D015153:Blotting, Western; D000939:Epitopes / Q000276:immunology; D004926:Escherichia coli / Q000201:enzymology; D005033:Ethylmaleimide / Q000494:pharmacology; D006801:Humans; D008105:Liver Cirrhosis, Biliary / Q000276:immunology*; D008970:Molecular Weight; D010446:Peptide Fragments / Q000276:immunology; D011768:Pyruvate Dehydrogenase Complex / Q000276:immunology*; D011773:Pyruvates / Q000378:metabolism; D019289:Pyruvic Acid; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D008063:Thioctic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D014357:Trypsin
D000941:Antigens; D001323:Autoantibodies; D000939:Epitopes; D010446:Peptide Fragments; D011768:Pyruvate Dehydrogenase Complex; D011773:Pyruvates; D008063:Thioctic Acid; D019289:Pyruvic Acid; C013091:lipoamide; D014357:Trypsin; D005033:Ethylmaleimide