title: Pancreatic nerve stimulation releases neuropeptide Y- but not galanin- or calcitonin gene-related peptide-like immunoreactivity from the pig pancreas.
abstract: We investigated the possible release of galanin-, calcitonin gene-related peptide (CGRP)-, and neuropeptide Y (NPY)-like immunoreactivity from the pancreatic nerves in thiopental-anaesthetized pigs. Ten minutes stimulation of the mixed autonomic pancreatic nerves during infusion of atropine (8 or 40 Hz, 5 ms, 10 mA, n = 5) inhibited insulin secretion, during both normoglycemia (9.1 +/- 0.2 mmol/l) and hyperglycemia (28.1 +/- 0.4 mmol/l). Concomitantly, pancreatic venous concentrations of NPY-like immunoreactivity increased. For example during normoglycemia, a nerve stimulation by 8 Hz increased the pancreatic venous levels of NPY-like immunoreactivity from 294 +/- 26 pmol/l to 391 +/- 23 pmol/l (P less than 0.001). In contrast, the pancreatic venous concentrations of galanin- or CGRP-like immunoreactivity did not change during the nerve stimulation. We conclude that electrical pancreatic nerve stimulation in the pig releases NPY-like immunoreactivity without affecting the pancreatic venous concentrations of galanin- or CGRP-like immunoreactivity.
authors: Department of Surgery, Lund University, Sweden.
authors: B
authors: B
authors: Ahrén
authors: H
authors: H
authors: Mårtensson
authors: R
authors: R
authors: Ekman
mesh_terms: D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001341:Autonomic Nervous System / Q000187:drug effects / Q000502:physiology*; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism*; D004558:Electric Stimulation; D019004:Galanin; D009478:Neuropeptide Y / Q000378:metabolism*; D010179:Pancreas / Q000294:innervation / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D013552:Swine
chemical_list: D009478:Neuropeptide Y; D010455:Peptides; D001285:Atropine; D019004:Galanin; D015740:Calcitonin Gene-Related Peptide