Identification and characterization of a hamster monoclonal antibody anti-2.28 directed against a 70-kilodalton activation antigen on human monocytes.
Hamster mAb against activated human monocytes were examined for their reactivities against monocyte activation Ag. One mAb, anti-2.28, stained only monocytes activated with LPS plus IFN-gamma, but not unactivated peripheral blood monocytes, polymorphonuclear leukocytes, lymphocytes, RBC, and platelets. However, it stained peripheral blood T cells activated with PMA plus anti-CD3 and peripheral blood and tonsillar B cells activated with PMA plus anti-mu. Of the 35 cell lines of diverse origin examined for immunofluorescence staining by anti-2.28, only EBV-transformed cell lines showed strong staining by this mAb. One pre-B cell line, Nalm-12, could be induced by PMA to exhibit intermediate staining. Immunoprecipitation studies identified the 2.28 Ag as a 70- to 85-kDa monomer. Immunofluorescence staining, immunoprecipitation, and peptide mapping studies indicated that 2.28 was different from a number of monocyte and lymphocyte surface Ag including Mo3e, B-4 (CD19), B-5, CD39, and the G28-8 Ag Bgp 95. These studies suggest that 2.28 may be a novel hemopoietic non-lineage-specific activation Ag.
Sung
S S
SS
Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City 73104.
Walters
J A
JA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification*; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface / Q000276:immunology* / Q000302:isolation & purification; D001402:B-Lymphocytes / Q000032:analysis; D002460:Cell Line; D006224:Cricetinae; D006412:Hematopoietic Stem Cells / Q000276:immunology; D006801:Humans; D008262:Macrophage Activation; D051379:Mice; D008970:Molecular Weight; D009000:Monocytes / Q000032:analysis / Q000276:immunology*; D014066:Palatine Tonsil; D011233:Precipitin Tests; D013194:Staining and Labeling; D013601:T-Lymphocytes / Q000032:analysis
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface