Mouse placental 57-kDa calcium-binding protein: I. Cloning of cDNA and characterization of developmental expression.
A cDNA clone to the mouse placental 57-kDa calcium-binding protein (MCaBP) [29] was isolated by immunoscreening a mouse placenta cDNA library constructed in the expression phage vector, lambda gt 11. The MCaBP cDNA was 0.7 kb in size, with restriction sites for StuI and Bg/II, and its identity to the MCaBP was confirmed by mRNA hybrid selection. RNA blot hybridization revealed a predominant, 3.9-kb transcript of the MCaBP in day-18 mouse placenta. The expression of the MCaBP during development was analyzed with respect to the levels of protein activity, translatable MCaBP mRNA, and total MCaBP transcript.
Tuan
R S
RS
Department of Biology, University of Pennsylvania, Philadelphia 19104.
Kirwin
J J
JJ
D000818:Animals; D000906:Antibodies; D010582:Bacteriophage lambda; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000235:genetics*; D003001:Cloning, Molecular; D004247:DNA; D004587:Electrophoresis, Agar Gel; D005260:Female; D005822:Genetic Vectors; D005865:Gestational Age; D007158:Immunologic Techniques; D051379:Mice; D009693:Nucleic Acid Hybridization; D010920:Placenta / Q000378:metabolism*; D010926:Placental Hormones / Q000032:analysis / Q000235:genetics*; D010957:Plasmids; D011247:Pregnancy; D012313:RNA / Q000302:isolation & purification; D012333:RNA, Messenger; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics
D000906:Antibodies; D002135:Calcium-Binding Proteins; D010926:Placental Hormones; D012333:RNA, Messenger; D011994:Recombinant Proteins; D012313:RNA; D004247:DNA; D002118:Calcium