Staining of neuroendocrine cells by Linder's argyrophil method.
Linder's argyrophil method, recently developed to stain nervous structures, is useful in the histochemical study of amine- and/or peptide-producing neuroendocrine (APUD) cells. On sections from various organs of four animal species Linder's method worked well and rapidly stained the neuroendocrine cells yellow, red or black; it stained black nervous structures against a pale yellow background. Double staining of single sections from Bouin-fixed gastric mucosa of rabbits demonstrated the correspondence of both Linder- and Grimelius-positive cells. Rapidity of application, intensity of impregnation and reproducibility in results are the best features of Linder's method when applied to the study of the neuroendocrine system.
Cecio
A
A
Institute of Anatomy, Faculty of Veterinary Medicine, University of Naples, Italy.
Vittoria
A
A
Budetta
G
G
Corona
M
M
D000818:Animals; D003370:Coturnix / Q000033:anatomy & histology*; D006651:Histocytochemistry / Q000379:methods*; D008854:Microscopy, Electron; D009490:Neurosecretory Systems / Q000648:ultrastructure*; D011784:Quail / Q000033:anatomy & histology*; D011817:Rabbits; D012756:Sheep; D013194:Staining and Labeling