title: Cloning of Plasmodium yoelii genes expressing three different sporozoite-specific antigens.
abstract: Genomic DNA isolated from Plasmodium yoelii (strain #17XNL) was prepared by partial digestion and cloned in Escherichia coli TB-1 with pUC18 plasmid. Antigen-producing recombinants were detected by a battery of monoclonal antibodies against antigens of the sporozoite stages. Four clones producing stage-specific sporozoite antigens were identified. One produced P. yoelii circumsporozoite protein, and three produced other P. yoelii sporozoite antigens.
authors: Infectious Diseases Program Center, Naval Medical Research Institute, Bethesda, Maryland 20814-5055.
authors: A
authors: A
authors: Wortman
authors: P
authors: P
authors: Rogers
authors: Y
authors: Y
authors: Charoenvit
authors: A
authors: A
authors: McDermott
authors: M
authors: M
authors: Leef
authors: M
authors: M
authors: Sedegah
authors: R L
authors: RL
authors: Beaudoin
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000096:biosynthesis / Q000235:genetics*; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D000939:Epitopes / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010967:Plasmodium yoelii / Q000235:genetics* / Q000276:immunology; D015183:Restriction Mapping
chemical_list: D000953:Antigens, Protozoan; D000939:Epitopes; D004247:DNA