title: Simple staining for heat-fixed brain sections after topographical pH analysis or autoradiographic studies.
abstract: Powerful topographic techniques are now available, among which autoradiographic and fluorescent mapping are the most prevalent. These techniques produce images that usually do not correlate with brain anatomy; subsequent staining is required to allow a precise association between the parameter(s) investigated and brain structures. A simple staining procedure is described, compatible with heat-fixed brain sections, and that was found particularly valuable in association with topographical tissue pH studies using the fluorescent pH indicator umbelliferone.
authors: Gough-Cooper Department of Neurological Surgery, Institute of Neurology, London, England.
authors: N A
authors: NA
authors: Parkinson
authors: G
authors: G
authors: Christofi
authors: T P
authors: TP
authors: Obrenovitch
authors: L
authors: L
authors: Symon
mesh_terms: D001345:Autoradiography; D001921:Brain / Q000033:anatomy & histology*; D006863:Hydrogen-Ion Concentration; D007211:Indoles; D013194:Staining and Labeling
chemical_list: D007211:Indoles; C018588:Luxol Fast Blue MBS