Formation of double-walled microtubules and multilayered tubulin sheets by basic proteins.
Some basic proteins enable microtubule protein to form special assembly products in vitro, known as double-walled microtubules. Using histones (H1, core histones) as well as the human encephalitogenic protein to induce the formation of double-walled microtubules, we made the following electron microscopic observations: (1) Double-walled microtubules consist of an "inner" microtubule which is covered by electron-dense material, apparently formed from the basic protein, and by a second tubulin wall. (2) The tubulin of the second wall seems to be arranged as protofilaments, surrounding the inner microtubule in a helical or ring-like manner. (3) The surface of double-walled microtubules lacks the projections of microtubule-associated proteins, usually found on microtubules. (4) In the case of protofilament ribbons (incomplete microtubules), H1 binds exclusively to their convex sides that correspond to the surface of microtubules. Zn2+-induced tubulin sheets, consisting in contrast to microtubules of alternately arranged protofilaments, are covered by H1 on both surfaces. Furthermore, multilayered sheet aggregates appeared. The results indicate that the basic proteins used interact only with that protofilament side which represents the microtubule surface. In accordance with this general principle, models on the structure of double-walled microtubules and multilayered tubulin sheets were derived.
Unger
E
E
Central Institute of Microbiology and Experimental Therapy, Academy of Sciences of the GDR, Jena.
Böhm
K J
KJ
Müller
H
H
Grossman
H
H
Fenske
H
H
Vater
W
W
D000818:Animals; D001921:Brain / Q000378:metabolism; D006657:Histones / Q000494:pharmacology*; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D008868:Microtubule Proteins / Q000378:metabolism*; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D004676:Myelin Basic Protein / Q000494:pharmacology*; D013552:Swine; D014404:Tubulin / Q000378:metabolism*
D006657:Histones; D046911:Macromolecular Substances; D008868:Microtubule Proteins; D004676:Myelin Basic Protein; D014404:Tubulin