Expression of domains of mouse alpha-fetoprotein in Escherichia coli.
A mouse alpha-fetoprotein complementary DNA containing the coding region for amino acids 256 to 548 was fused to the lac transcriptional and translational control elements contained on the expression vector pOP203-28. The expression of a 35 kD hybrid lac Z'- alpha-fetoprotein polypeptide in Escherichia coli was demonstrated by the chloramphenicol release assay and by immunoprecipitation using rabbit anti-mouse alpha-fetoprotein antibodies as probe.
Boismenu
R
R
Department of Microbiology and Immunology, McGill University, Montreal, Canada.
Dubow
M S
MS
Murgita
R A
RA
D000595:Amino Acid Sequence; D000818:Animals; D004247:DNA / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics*; D005786:Gene Expression Regulation; D051379:Mice; D008969:Molecular Sequence Data; D010957:Plasmids; D011994:Recombinant Proteins / Q000032:analysis; D000509:alpha-Fetoproteins / Q000096:biosynthesis / Q000235:genetics*
D011994:Recombinant Proteins; D000509:alpha-Fetoproteins; D004247:DNA