The perfused porcine pancreas as a model for testing organ protective solutions.
The present study was designed to establish an in vitro perfused porcine pancreas preparation as a model for testing the effect of organ protective solutions on stimulated pancreatic endocrine and exocrine secretion. The pancreas was prepared and perfused for 10 min with Euro Collins solution, thereafter it was stored in the cold (4 degrees C) for various times. After 3-h and 6-h ischemia pancreatic insulin release in response to glucose was not significantly affected. After 12-h ischemia reduced pancreatic insulin secretin, increased perfusion pressure, and increased amylase and lipase release indicated pancreatic damage. Complete pancreatic dysfunction was seen after 24-h and 48-h ischemia with massive increase in perfusion pressure and low insulin secretion which did not follow a glucose-dependent release pattern, while amylase and lipase concentrations in the perfusion medium increased. Stimulated exocrine pancreatic secretion was significantly decreased already after 3-h ischemia and completely lost after 12 h.
Barthel
M
M
Dept. of Medicine, University of Göttingen, Federal Republic of Germany.
Leonhardt
U
U
Köhler
H
H
Siegel
E G
EG
Tytko
A
A
Nebendahl
K
K
Peiper
H J
HJ
Creutzfeldt
W
W
D000681:Amylases / Q000032:analysis; D000818:Animals; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007511:Ischemia / Q000503:physiopathology; D008049:Lipase / Q000032:analysis; D008954:Models, Biological; D009926:Organ Preservation; D010101:Oxygen Consumption; D010179:Pancreas / Q000098:blood supply / Q000502:physiology*; D010189:Pancreatic Juice / Q000378:metabolism; D010477:Perfusion; D013552:Swine; D013997:Time Factors
D007328:Insulin; D008049:Lipase; D000681:Amylases