The in vitro antiviral activity of isoprinosine on simian rotavirus (SA-11).
1. The antiviral effect of isoprinosine on simian rotavirus (SA-11) replication was studied using MA-104 cell cultures from Rhesus monkey fetal kidney. 2. Isoprinosine (N,N-dimethylamino-2-propanol-p-acetamidobenzoate in association with inosine) added after viral infection (therapeutic test) inhibited viral replication by more than 90%. In these experiments, the drug was added to the medium and replaced daily at concentrations varying from 62.5 micrograms/ml to 1 mg/ml. Viral inhibition activity was dependent on drug concentration. No antiviral effect was observed when isoprinosine was tested without replacement (200-500 micrograms/ml). 3. When isoprinosine (1 mg/ml) was added to cell cultures only before viral infection (prophylactic test), inhibition of viral replication occurred but was less than 90%. Inhibition by less than 90% is not considered to be significant in this type of test. 4. Isoprinosine inhibited synthesis of both viral antigen (protein) and viral double-stranded nucleic acid, as monitored by immunofluorescence and acridine orange staining, respectively. Inhibition of synthesis of viral macromolecules increased with drug concentration.
Linhares
R E
RE
Departamento de Virologia, Universidade Federal do Rio de Janeiro, Brasil.
Wigg
M D
MD
Lagrota
M H
MH
Nozawa
C M
CM
D000165:Acridine Orange / Q000494:pharmacology; D002478:Cells, Cultured / Q000187:drug effects; D003470:Culture Media; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D066298:In Vitro Techniques; D007288:Inosine / Q000031:analogs & derivatives*; D007542:Inosine Pranobex / Q000494:pharmacology*; D008856:Microscopy, Fluorescence; D012401:Rotavirus / Q000502:physiology*; D014779:Virus Replication / Q000187:drug effects*
D003470:Culture Media; D007288:Inosine; D000165:Acridine Orange; D007542:Inosine Pranobex