title: Inhibition of in vitro and in vivo mast cell degranulation by Taenia crassiceps metacestodes in vitro incubation products.
abstract: In vitro released products of T. crassiceps metacestodes (TcIP) harvested from the peritoneal cavity of NMRI mice were tested for inhibitory effects on the in vitro degranulation of peritoneal mast cells (MCs) of normal mice (NMRI) and rats (Wistar) and on the in vivo degranulation of rat (Wistar) skin MCs (PCA-assay). In vitro degranulation was elicited chemically (compound 48/80, polymyxin B or the bee venom peptide, mellitin). In vivo degranulation was triggered immunologically (anaphylactic systems ovalbumin/anti-ovalbumin or Fasciola hepatica crude fluke extract antigen/serum of fluke-infected rats (Wistar]. In vitro degranulation of murine peritoneal MCs or the in vitro histamine release of rat peritoneal MCs normally induced chemically was significantly inhibited when the MCs were preincubated with the TcIP or with serum of T. crassiceps-infected NMRI mice from day 35 post infection and thereafter. In vitro degranulation of peritoneal MCs of infected mice was strongly inhibited beginning on day 10 after infection. Also in vivo degranulation of the IgE-sensitized rat skin MCs was significantly reduced by intradermal injection of the TcIP before (6, 3 and 1 h) antigen challenge and by preinjection (1 h) of serum from infected mice (day 80 p.i.)-The inhibitory effect was also demonstrated after immunoadsorption of mouse serum proteins naturally contaminating the TcIP. Heating (100 degrees C/15 min), even in the presence of 0.25 M HCl, did not suppress the inhibitory activity.
authors: Fachbereich Biologie, Philipps-Universität, Marburg.
authors: B
authors: B
authors: Seifert
authors: E
authors: E
authors: Geyer
mesh_terms: D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects; D002846:Chromatography, Affinity; D005260:Female; D006636:Histamine Release; D006358:Hot Temperature; D006851:Hydrochloric Acid / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology*; D008555:Melitten / Q000494:pharmacology; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology; D011112:Polymyxin B / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013621:Taenia / Q000276:immunology*; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
chemical_list: D008555:Melitten; D003189:p-Methoxy-N-methylphenethylamine; D011112:Polymyxin B; D006851:Hydrochloric Acid