Multiple effects of human recombinant interleukin 4 on human myeloid monocyte cell lines.
The effects of recombinant human interleukin 4 (rIL-4) on proliferation and differentiation on human myeloid/monocytic leukemia cell lines were examined. At high concentrations, rIL-4 had a slight enhancing effect on [3H]thymidine incorporation by U937 cells. rIL-4 markedly induced expression of the Fc epsilon receptor (CD23) and the Leu-M3 antigen (CD14) on U937 cells. HL60 and THP-1 cells treated with rIL-4 also showed increased CD23 expression, but little change of CD14 antigen expression. CD23 induction required lower amounts of IL-4 than needed for T cell growth, indicating that CD23 induction on U937 will serve as a sensitive assay for human IL-4. rIL-4 reduced the steady state level of IL-1 beta mRNA in U937.
Noma
T
T
Department of Medical Chemistry, Faculty of Medicine, Kyoto University, Japan.
Nakakubo
H
H
Hama
K
K
Honjo
T
T
D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis*; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis*; D015152:Blotting, Northern; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D006801:Humans; D015847:Interleukin-4; D007378:Interleukins / Q000494:pharmacology*; D018950:Lipopolysaccharide Receptors; D009000:Monocytes / Q000166:cytology*; D011961:Receptors, Fc / Q000032:analysis*; D017455:Receptors, IgE; D011994:Recombinant Proteins
D000944:Antigens, Differentiation, B-Lymphocyte; D015214:Antigens, Differentiation, Myelomonocytic; D007378:Interleukins; D018950:Lipopolysaccharide Receptors; D011961:Receptors, Fc; D017455:Receptors, IgE; D011994:Recombinant Proteins; D015847:Interleukin-4