title: Secretin-induced exocrine secretion in perfused pancreas isolated from diabetic rats.
abstract: Exocrine secretory function in response to 10 pM to 10 nM synthetic secretin was evaluated in perfused pancreas isolated from control, streptozocin-induced diabetic (STZ-D), alloxan-induced diabetic (ALX-D), and insulin-treated STZ-D rats. In STZ-D rats, the basal rate of pancreatic juice flow was significantly increased (10.3 +/- 1.0 microliters/20 min) compared with control rats (4.4 +/- 0.2 microliters/20 min). The basal rate of amylase output as well as pancreatic amylase content were significantly decreased to less than 5% of control values. The basal rates of protein and trypsinogen outputs were similar in both groups. In both control and diabetic rats, secretin caused a dose-dependent increase in exocrine secretion. Secretin (10 pM to 10 nM) induced 1.1- to 11.7-fold increases in exocrine secretion in STZ-D rats. These increases were significantly lower than the 2.1- to 20.8-fold increases in control rats. Furthermore, there was no significant increase in exocrine secretion from STZ-D rats in response to 10 pM secretin, although this concentration of secretin caused a significant increase in control rats. Secretin-induced exocrine secretion in ALX-D rats was similar to that in STZ-D rats. In insulin-treated STZ-D rats, the basal rates of pancreatic secretion were not significantly different from those of control rats. These results suggest that insulin resistance in this patient was due to a circulating factor of low molecular weight that uncoupled insulin stimulation of glucose transport from receptor binding and phosphorylation. The factor appears to increase the binding activity of the alpha-subunit of the insulin receptor without affecting the kinase activity of the beta-subunit.
authors: Second Department of Internal Medicine, Kobe University School of Medicine, Japan.
authors: Y
authors: Y
authors: Okabayashi
authors: M
authors: M
authors: Otsuki
authors: A
authors: A
authors: Ohki
authors: T
authors: T
authors: Nakamura
authors: S
authors: S
authors: Tani
authors: S
authors: S
authors: Baba
mesh_terms: D000681:Amylases / Q000378:metabolism; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D066298:In Vitro Techniques; D008297:Male; D010101:Oxygen Consumption / Q000187:drug effects; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism* / Q000503:physiopathology; D010189:Pancreatic Juice / Q000187:drug effects / Q000378:metabolism; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D012633:Secretin / Q000494:pharmacology*
chemical_list: D012633:Secretin; D000681:Amylases