title: Total and antigen-specific serum immunoglobulin isotype concentrations in hyperimmunized cattle that have undergone plasmapheresis.
abstract: The effects of prolonged plasmapheresis of cattle on total and antigen-specific immunoglobulin production were evaluated. Five adult cows were hyperimmunized by repeated IV administration of live, logarithmic-phase Pasteurella haemolytica A1 organisms. Three of the cows underwent plasmapheresis daily for 3 weeks. From 2 cows, serum was only obtained periodically. Anti-P haemolytica antibody was assayed by indirect hemagglutination and a kinetic-augmented, antigen-capture ELISA for capsular polysaccharide and lipopolysaccharide/outer membrane protein antigens. Total serum immunoglobulin concentration was determined for IgM, IgG1, and IgG2 by primary radial immunodiffusion. Anti-P haemolytica A1 activity increased rapidly after immunization. After beginning plasmapheresis, the antigen-specific antibody activities remained nearly constant. In general, antilipopolysaccharide/outer membrane protein activity (in terms of concentration) was higher than anti-capsular polysaccharide activity and was not affected as much by the plasmapheresis. Total serum Ig concentration decreased transiently by a small amount after beginning plasmapheresis.
authors: Department of Veterinary Microbiology and Parasitology, College of Veterinary Medicine, Texas A&M University, College Station 77843.
authors: D S
authors: DS
authors: McVey
authors: R W
authors: RW
authors: Loan
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D002417:Cattle / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D000939:Epitopes; D005260:Female; D007114:Immunization / Q000662:veterinary*; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D007132:Immunoglobulin Isotypes / Q000032:analysis* / Q000276:immunology; D007075:Immunoglobulin M / Q000032:analysis / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D010325:Pasteurella / Q000276:immunology*; D010956:Plasmapheresis / Q000662:veterinary*; D011135:Polysaccharides, Bacterial / Q000276:immunology; D013997:Time Factors
chemical_list: D001425:Bacterial Outer Membrane Proteins; D000939:Epitopes; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial