Isolation and characterization of two homologous cDNA clones from Torpedo electromotor neurons.
Two homologous cDNA clones were isolated from a Torpedo california electric lobe lambda gt11 expression library using a polyclonal antiserum directed against proteins associated with synaptic vesicles. Northern blotting reveals an 8- to 9-kb transcript in the electric lobe and the spinal cord, but not in the brain or other non-neuronal tissues. Antibodies generated against a fusion protein synthesized in Escherichia coli reacted with a 85- to 90-kD species in the neurons of the electric lobe. The immunoreactivity is associated with microsomal membranes and can be extracted readily with high salt. Immunohistochemical studies demonstrated a sparse punctate staining pattern in the cell body which colocalized with a subpopulation of post-Golgi vesicles.
Ngsee
J K
JK
Department of Biological Sciences, Stanford University, CA 94305-5020.
Scheller
R H
RH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002458:Cell Fractionation; D003001:Cloning, Molecular; D004247:DNA; D008969:Molecular Sequence Data; D009046:Motor Neurons; D009419:Nerve Tissue Proteins / Q000235:genetics*; D012313:RNA / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid; D014101:Torpedo / Q000235:genetics*
D009419:Nerve Tissue Proteins; D012313:RNA; D004247:DNA