Fluoroaluminates stimulate histamine secretion in the digitonin-permeabilized rat mast cells.
The combination of NaF and AlCl3 stimulated histamine secretion from digitonin-permeabilized rat mast cells only in the presence of trace amount of Ca2+. When NaF plus AlCl3 were added together with a maximal dose of guanosine-5'-[gamma-thio]triphosphate (GTP gamma S), the combined effect was less than additive. The results suggest that the secretory effect of fluoroaluminates is, at least partially, mediated by GTP-binding protein, which is previously found to be activated by GTP gamma S.
Sorimachi
M
M
Department of Physiology, Kagoshima University, School of Medicine, Japan.
Nishimura
S
S
Yamagami
K
K
Yada
T
T
D000535:Aluminum / Q000494:pharmacology*; D000077410:Aluminum Chloride; D017607:Aluminum Compounds; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology*; D004072:Digitonin; D005260:Female; D019204:GTP-Binding Proteins / Q000378:metabolism; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects*; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012969:Sodium Fluoride / Q000494:pharmacology*; D013873:Thionucleotides / Q000494:pharmacology
D017607:Aluminum Compounds; D002712:Chlorides; D013873:Thionucleotides; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D000077410:Aluminum Chloride; D006160:Guanosine Triphosphate; D012969:Sodium Fluoride; D000535:Aluminum; D019204:GTP-Binding Proteins; D004072:Digitonin; D002118:Calcium