Monoclonal antibodies to a synthetic peptide corresponding to a repeated sequence in the Plasmodium falciparum antigen Pf155.
Mouse monoclonal antibodies were prepared against a synthetic peptide (EENVEHDA) corresponding to a tandemly repeated sequence in the C-terminus of the Plasmodium falciparum antigen Pf155. One antibody (IgG1) producing hybridoma was studied in detail. The specificity of the antibody was determined by enzyme-linked immunosorbent assays using bovine serum albumin-conjugated or free peptides as solid phase antigens and various synthetic peptides for inhibition. The antibody reacted with Pf155 as detected by immunofluorescence and immunoblotting. It was also an efficient inhibitor of merozoite invasion in P. falciparum in vitro cultures indicating that it defines a biologically important epitope present on the native Pf155 molecule.
Ruangjirachuporn
W
W
Department of Immunology, University of Stockholm, Sweden.
Wåhlin
B
B
Perlmann
H
H
Carlsson
J
J
Berzins
K
K
Wahlgren
M
M
Udomsangpetch
R
R
Wigzell
H
H
Perlmann
P
P
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D000953:Antigens, Protozoan / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006825:Hybridomas; D007118:Immunoassay; D010455:Peptides / Q000276:immunology*; D010963:Plasmodium falciparum / Q000276:immunology*; D012091:Repetitive Sequences, Nucleic Acid
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D000939:Epitopes; D010455:Peptides