Hemoglobin synthesis of both adult and fetal types in a human CML cell line.
We present evidence for the continuous erythroid differentiation of a cell line, KU-812-F, without the addition of an inducer. Erythroid differentiation of these cells was confirmed according to the following findings: 1) erythroid morphology; 2) existence of glycophorin A and carbonic anhydrase I as a membrane marker protein and an enzyme characteristic of erythroid cells, respectively; 3) synthesis of adult type (HbA and HbA2) and fetal type (HbF) hemoglobins, as detected on isoelectric focusing; and 4) transcription of the alpha-, beta-, and gamma-globin genes, as detected on Northern blot analysis.
Okano
H
H
Kyushu Cancer Center Research Institute, Fukuoka.
Endo
T
T
Shiokawa
S
S
Kyoden
Y
Y
Ishibashi
Y
Y
Kono
A
A
Nishi
K
K
Fukumaki
Y
Y
D002454:Cell Differentiation; D002460:Cell Line; D005319:Fetal Hemoglobin / Q000096:biosynthesis*; D006441:Hemoglobin A / Q000096:biosynthesis*; D006443:Hemoglobin A2 / Q000096:biosynthesis*; D006801:Humans
D006441:Hemoglobin A; D006443:Hemoglobin A2; D005319:Fetal Hemoglobin