Depression of cytochrome P450-dependent drug biotransformation by poly(rI.rC) and aspirin.
The inhibition of hepatic microsomal cytochrome P450 and cytochrome b5 levels by poly(rI.rC) and aspirin in vitro was studied in male Swiss mice. Poly(rI.rC) (10 mg/kg) decreases cytochrome P450 to a level of 61% and cytochrome b5 to 31%, while the activity of aminopyrine demethylase was decreased to 66%. Aspirin (200 mg/kg) decreased the level of cytochrome P450 and cytochrome b5 to 45 and 23%, respectively. Further studies on the effect of poly(rI.rC) showed that this decrease in the levels of cytochromes was mainly due to alteration in the protein synthesis of these hemoproteins.
Jagota
S K
SK
Department of Biochemistry, Panjab University, Chandigarh.
D000596:Amino Acids / Q000378:metabolism; D000633:Aminopyrine N-Demethylase / Q000378:metabolism; D000818:Animals; D001241:Aspirin / Q000494:pharmacology*; D001711:Biotransformation; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D003573:Cytochrome b Group / Q000378:metabolism; D015786:Cytochromes b5; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D011070:Poly I-C / Q000494:pharmacology*; D012313:RNA / Q000378:metabolism
D000596:Amino Acids; D003573:Cytochrome b Group; D004364:Pharmaceutical Preparations; D012313:RNA; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System; D000633:Aminopyrine N-Demethylase; D011070:Poly I-C; D001241:Aspirin