Discontinuous buffer systems optimized for the agarose gel electrophoresis of subcellular particles.
Discontinuous buffer systems operative between pH 5.7 and 7.4, 0 degrees C, were generated, which are characterized by more rapidly displaced moving boundaries than applied previously. These allow one to resolve subcellular particles relatively rapidly and at relatively low agarose gel concentrations. A commercial mixture of DNA restriction fragments pre-stained with ethidium bromide was found to be a suitable tracking dye for these boundaries.
Orbán
L
L
Section on Macromolecular Analysis, National Institute of Child Health and Human Development, Bethesda, MD 20892.
Hahn
E
E
Chrambach
A
A
D002021:Buffers; D004396:Coloring Agents; D004247:DNA / Q000032:analysis; D004586:Electrophoresis; D004587:Electrophoresis, Agar Gel; D004996:Ethidium; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D013194:Staining and Labeling; D013347:Subcellular Fractions / Q000032:analysis*
D002021:Buffers; D004396:Coloring Agents; D004247:DNA; D004996:Ethidium