title: [Preparation of a suspension of isolated hepatocytes using the Soviet drug collalitin].
abstract: Isolation of individual hepatocytes suspension from rats was carried out using trypsin, collagenase and collalytine; maximal yield of the viable hepatocytes was obtained when 0.05% solution of collagenase was used. Trypsin was ineffective in production of viable cells, while collalytine from pig pancreas exhibited the satisfactory results at concentration of 0.15-0.20%.
authors: V Iu
authors: VIu
authors: Toshchakov
authors: M A
authors: MA
authors: Danilov
authors: N K
authors: NK
authors: Shestakova
authors: T I
authors: TI
authors: Kozlovskaia
authors: E Iu
authors: EIu
authors: Kulikova
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods*; D008099:Liver / Q000166:cytology*; D003012:Microbial Collagenase; D051381:Rats; D013194:Staining and Labeling; D014357:Trypsin
chemical_list: D014357:Trypsin; D003012:Microbial Collagenase
vernacular_title: Poluchenie suspenzii izolirovannykh gepatotsitov s pomoshch'iu otechestvennogo preparata kollalitina.