Tumor necrosis factor and IFN induce a common set of proteins.
The treatment of cells with TNF or IFN results in the development of an antiviral state and in the induction of a common set of proteins with m.w. of 80,000, 67,000, and 56,000. The induction of the 80,000- and 56,000-Da proteins after TNF treatment is dependent on the synthesis of an intermediary protein, whereas the induction of the 67,000-Da protein appears to occur as a direct result of the TNF treatment. The effects of antibodies to IFN on the TNF-mediated effects have been evaluated and reveal that the incubation of TNF-treated cells with antibody to rIFN-beta 1 greatly reduces the antiviral effectiveness of the TNF treatment and blocks the ability of TNF to induce the 80,000-Da protein. Incubation with antibodies to either IFN-alpha or IFN-gamma failed to affect the TNF-mediated responses. Thus, the induction by TNF of each of the proteins is regulated differently and is mediated through both IFN-dependent and IFN-independent mechanisms.
Rubin
B Y
BY
Department of Lymphokine Biology, New York Blood Center 10021.
Anderson
S L
SL
Lunn
R M
RM
Richardson
N K
NK
Hellermann
G R
GR
Smith
L J
LJ
Old
L J
LJ
D000818:Animals; D001666:Binding Sites, Antibody; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007372:Interferons / Q000276:immunology / Q000494:pharmacology*; D008715:Methionine / Q000494:pharmacology; D051379:Mice; D008970:Molecular Weight; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D011506:Proteins / Q000302:isolation & purification; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D011506:Proteins; D014409:Tumor Necrosis Factor-alpha; D007372:Interferons; D003513:Cycloheximide; D008715:Methionine