The contribution of ATP turnover by the Na+/K+-ATPase to the rate of respiration of hepatocytes. Effects of thyroid status and fatty acids.
In less than 1 min ouabain maximally inhibits oxygen consumption due to gramicidin-induced ATP turnover by the Na+/K+-ATPase in hepatocytes. Ouabain rapidly inhibits respiration on palmitate or glucose by only 6-10% indicating that the Na+/K+-ATPase plays a minor role in cell ATP turnover. 29% of the extra oxygen consumption of hepatocytes isolated from hyperthyroid rats was inhibited by ouabain showing that the Na+/K+-ATPase is responsible for some but not the majority of the stimulation of respiration induced by thyroid hormone.
Nobes
C D
CD
Department of Biochemistry, University of Cambridge, U.K.
Lakin-Thomas
P L
PL
Brand
M D
MD
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002210:Caprylates / Q000494:pharmacology*; D002478:Cells, Cultured; D005215:Fasting; D005260:Female; D006096:Gramicidin / Q000494:pharmacology; D006980:Hyperthyroidism / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology; D010101:Oxygen Consumption / Q000187:drug effects; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D012413:Rubidium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013961:Thyroid Gland / Q000502:physiology*
D002210:Caprylates; D010169:Palmitic Acids; D006096:Gramicidin; D019308:Palmitic Acid; D010042:Ouabain; D000255:Adenosine Triphosphate; D000254:Sodium-Potassium-Exchanging ATPase; D012413:Rubidium