Primary structure of belladonna mottle virus coat protein.
The coat protein of belladonna mottle virus (a tymovirus) was cleaved by trypsin and chymotrypsin, and the peptides were separated by high performance liquid chromatography using a combination of gel permeation, reverse phase, and ion pair chromatography. The peptides were sequenced manually using the 4-N, N-dimethylaminoazobenzene-4'-isothiocyanate/phenyl isothiocyanate double-coupling method. The chymotryptic peptides were aligned by overlapping sequences of tryptic peptides and by homology with another tymovirus, eggplant mosaic virus. The belladonna mottle virus is more closely related to eggplant mosaic virus than to turnip yellow mosaic virus, the type member of this group, as evident from the sequence homologies of 57 and 32%, respectively. The accumulation of basic residues at the amino terminus implicated in RNA-protein interactions in many spherical plant viruses was absent in all the three sequences. Interestingly, the amino-terminal region is the least conserved among the tymoviruses. The longest stretch of conserved sequence between belladonna mottle virus and eggplant mosaic virus was residues 34-44, whereas it was residues 96-102 in the case of belladonna mottle virus and turnip yellow mosaic virus. A tetrapeptide in the region (residues 154-157) was found to be common for all the three sequences. It is possible that these conserved regions (residues 34-44, 96-102, 154-157) are involved in either intersubunit or RNA-protein interactions.
Suryanarayana
S
S
Department of Biochemistry, Indian Institute of Science, Bangalore.
Rao
N A
NA
Murthy
M R
MR
Savithri
H S
HS
D000595:Amino Acid Sequence; D002213:Capsid / Q000032:analysis* / Q000276:immunology / Q000648:ultrastructure; D002851:Chromatography, High Pressure Liquid; D000939:Epitopes; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis; D010942:Plant Viruses / Q000032:analysis*; D011487:Protein Conformation; D012995:Solubility
D000939:Epitopes; D010446:Peptide Fragments