Synthesis of acute-phase proteins in perfused liver following administration of recombinant interleukin 1 alpha to normal or adrenalectomized rats.
The purpose of this study was to investigate the effects of recombinant interleukin 1 alpha (rIL-1 alpha) on metabolic rate and synthesis of acute-phase proteins in intact and adrenalectomized rats. Animals were housed in metabolic cages with daily recording of food intake and body weight. Twice daily, for 3 days, the rats were injected intraperitoneally with 5000 LAF U of human rIL-1 alpha, purified from Escherichia coli. Control animals were pair-fed and received corresponding injections with saline. In the morning of the fourth day, resting energy expenditure (REE) was determined by indirect calorimetry, and synthesis of total secreted proteins, albumin, complement component C3, and seromucoid fraction was measured by radioimmunological method using rat-specific antisera and [3H]leucine in livers perfused for 2 hr. Food intake decreased by approximately 30% during rIL-1 alpha administration to intact rats. The decrease in food intake occurred later and was less pronounced in adrenalectomized rats receiving rIL-1 alpha. Growth rate was significantly reduced on the first day of rIL-1 alpha treatment in intact rats, while there was no effect on growth rate in adrenalectomized animals. After rIL-1 alpha administration, REE was increased by 26% in intact rats (P less than 0.001) and by 14% in adrenalectomized rats (N.S.). Increased synthesis rates of total secreted proteins, complement component C3, and seromucoid fraction were observed in livers of intact rats following rIL-1 alpha administration. In adrenalectomized rats, only production of C3 was significantly increased after treatment with rIL-1 alpha. Albumin synthesis rate was not changed in either group following rIL-1 alpha injections.(ABSTRACT TRUNCATED AT 250 WORDS)
Pedersen
P
P
Department of Surgery, University of Cincinnati Medical Center, Ohio 45267-0558.
Hasselgren
P O
PO
Li
S J
SJ
Hiyama
D T
DT
Fischer
J E
JE
D000209:Acute-Phase Proteins / Q000096:biosynthesis*; D000311:Adrenal Glands / Q000502:physiology*; D000315:Adrenalectomy; D000818:Animals; D004435:Eating; D004734:Energy Metabolism; D007375:Interleukin-1 / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism*; D008297:Male; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D011994:Recombinant Proteins / Q000494:pharmacology*
D000209:Acute-Phase Proteins; D007375:Interleukin-1; D011994:Recombinant Proteins