Glycoproteins modulate adhesion in terminally differentiated keratinocytes.
The stratum corneum can be dissociated into single squames by homogenization in ether. We have reaggregated the free corneocytes into a multilayered lamellar structure resembling an intact stratum corneum. The reconstituted stratum corneum reacts with fluorescein-conjugated lectins, unlike the intact tissue. We infer that the lack of binding in the intact tissue is due to masking of saccharide sites by lipids (which are extracted by the ether). In an extension of the procedure, the ether is removed and replaced by acetone. This system permits us to modulate corneocyte reaggregation by the addition of appropriate agents. We have used this system to corroborate our hypothesis that a 40 kD cell-surface glycoprotein (an endogenous lectin specific for amino sugars), which we have isolated from the stratum corneum, is instrumental in adhesion of corneocytes by cross-linking with amino sugar sites on adjacent cells. The reaggregation is inhibited by the antibody to the 40 kD glycoprotein. It is also inhibited by either the addition of amino sugars which bind to the endogenous lectin, or the addition of exogenous lectins specific for amino sugars which bind to the ligand.
Brysk
M M
MM
Department of Dermatology, University of Texas Medical Branch, Galveston 77550.
Rajaraman
S
S
Penn
P
P
Barlow
E
E
D000906:Antibodies / Q000494:pharmacology; D002241:Carbohydrates / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002449:Cell Aggregation / Q000187:drug effects; D000078404:Epidermal Cells; D004817:Epidermis / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000502:physiology*; D006801:Humans; D007633:Keratins / Q000502:physiology*; D037102:Lectins / Q000494:pharmacology
D000906:Antibodies; D002241:Carbohydrates; D006023:Glycoproteins; D037102:Lectins; D007633:Keratins