Differential recognition by human cytotoxic T cell clones of human M1 fibroblasts transfected with an HLA-B7 gene (JY150) suggests the existence of two different HLA-B7 alleles in the cell line JY (HLA-A2,2;B7,7;Cw-,-;DR4,w6).
We have used a panel of human HLA-B7-specific CTL clones to identify an HLA-B7 gene (JY150) transfected into human M1 fibroblasts (M1/B7). Only a subset of the CTL clones recognized the M1/B7 cells, whereas all CTL clones recognized the donor of the B7 gene, the cell line JY (HLA-A2,2;B7,7;Cw-,-;DR4,w6). Analysis of the fine specificity of these CTL clones was performed by testing the reactivity on M1 cells transfected with an HLA-B27K gene and on a panel of cell lines typed for HLA-B7 subtypes (variants). These results, combined with one-dimensional IEF analysis of the M1/B7 cells and the B7 subtypes, indicated that the differential recognition by the CTL clones of the transfected gene was not caused by aberrant expression of the gene itself or due to the absence of critical accessory molecules on the M1 fibroblast cells. Our data suggest that the widely used HLA-B7 reference cell line JY is not homozygous at the HLA-B locus, but contains two different B7 alleles encoding the B7.2 and B7.4 subtypes.
van Seventer
G A
GA
Central Laboratory, Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Spits
H
H
Yssel
H
H
Melief
C J
CJ
Ivanyi
P
P
D000483:Alleles; D002460:Cell Line; D002999:Clone Cells / Q000145:classification / Q000276:immunology / Q000378:metabolism; D000939:Epitopes / Q000235:genetics; D005347:Fibroblasts / Q000276:immunology* / Q000378:metabolism; D005786:Gene Expression Regulation; D006680:HLA Antigens / Q000235:genetics*; D015789:HLA-A2 Antigen; D015793:HLA-B7 Antigen; D015236:HLA-C Antigens; D006684:HLA-DR Antigens / Q000235:genetics; D006801:Humans; D013602:T-Lymphocytes, Cytotoxic / Q000145:classification / Q000276:immunology* / Q000378:metabolism; D014162:Transfection
D000939:Epitopes; D006680:HLA Antigens; D015789:HLA-A2 Antigen; D015793:HLA-B7 Antigen; D015236:HLA-C Antigens; D006684:HLA-DR Antigens