[The effect of human gamma globulin on tbe production of antibodies to the surface antigen of the hepatitis B virus in an experiment on animals].
The biological assay for the determination of HBsAg in gamma globulin is proposed. The assay is based on the immunizing action of immune complexes and some specific features of secondary immune response. For this purpose, the highly purified preparation of HBsAg is injected into guinea pigs. Subsequently the animals receive human gamma globulin. An increase in the titer of anti-HBsAg antibodies in the animals preimmunized with the antigen is indicative of the presence HBsAg in human gamma globulin. In accordance with another variant of this assay, human gamma globulin is introduced into mice and 21 days later the animals are immunized with HBsAg. The appearance of anti-HBsAg antibodies in the animals, previously treated with gamma globulin, on day 4 after the injection of the antigen indicate that the animals experience the second penetration of HBsAg, i. e. its presence in previously injected human gamma globulin.
Gerasun
B A
BA
Fedechko
I M
IM
D000818:Animals; D001681:Biological Assay / Q000379:methods; D006168:Guinea Pigs; D006510:Hepatitis B Antibodies / Q000032:analysis / Q000096:biosynthesis*; D006514:Hepatitis B Surface Antigens / Q000008:administration & dosage / Q000276:immunology*; D006801:Humans; D007114:Immunization / Q000379:methods; D008297:Male; D051379:Mice; D013997:Time Factors; D005719:gamma-Globulins / Q000008:administration & dosage / Q000276:immunology*
D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D005719:gamma-Globulins
Vliianie chelovecheskogo gamma-globulina na produktsiiu antitel k poverkhnostnomu antigenu virusa gepatita B v éksperimente na zhivotnykh.