Characterization of two different genes (cDNA) for cytochrome c oxidase subunit VIa from heart and liver of the rat.
By antibody screening of a rat liver and a rat heart cDNA library in lambda gt11 two clones coding for the liver- and heart-specific subunit VIa of rat cytochrome c oxidase were isolated. In the heart cDNA sequence a TAA stop codon was found in frame 18 bp 5' upstream of the first methionine codon, thus excluding a leader sequence for this protein. The two cDNAs contain the full-length coding region of two subunits. The amino acid sequences of the two subunits show only 50% homology, whereas 74% homology was found between rat heart and bovine heart subunit VIa. By Northern blot analysis it is shown that the gene for subunit VIa from heart is only expressed in heart and skeletal muscle, whereas that from liver is also expressed in kidney, brain, heart and weakly in muscle.
Schlerf
A
A
Biochemie, Fb Chemie der Philipps-Universität, Marburg, FRG.
Droste
M
M
Winter
M
M
Kadenbach
B
B
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003062:Codon / Q000235:genetics; D004247:DNA / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D009206:Myocardium / Q000201:enzymology*; D021382:Protein Sorting Signals / Q000235:genetics; D012313:RNA / Q000032:analysis; D051381:Rats; D012689:Sequence Homology, Nucleic Acid
D003062:Codon; D021382:Protein Sorting Signals; D012313:RNA; D004247:DNA; D003576:Electron Transport Complex IV