title: Regulation of Trichophyton rubrum proteolytic activity.
abstract: Trichophyton rubrum is the most common dermatophyte of humans and normally colonizes the superficial layers of the epidermis (stratum corneum). Several proteinases with a possible role in the metabolism of host proteins have been purified from this fungus. The regulation of these enzymes and their role in fungal metabolism were studied at the biochemical level. General proteolytic (azocollytic) activity was repressed when log-phase cultures of T. rubrum were grown in a minimal medium that contained readily metabolized sources of carbon, nitrogen, sulfur, and phosphorus. When either carbon, nitrogen, or sulfur was deleted from this minimal medium, azocollytic activity was derepressed. In all cases a high-molecular-weight activity (Mr, greater than 200,000) was expressed. A 71,000-Mr proteinase was observed in nitrogen-depleted cultures, and proteolytic species of Mr 124,000 and 27,000 were secreted in sulfur-depleted cultures. The addition of either inorganic (MgSO4, Na2SO3, NaS2O3) or organic (methionine, cysteine) sulfur to the sulfur-depleted medium repressed the expression of azocollytic activity. In contrast, keratinolytic activity was not repressed by carbon, nitrogen, or sulfur but instead was induced when a protein source was included in the minimal medium. Stationary-phase cultures of T. rubrum secreted all proteolytic activities constitutively. Unlike log-phase cultures, the stationary-phase cultures secreted azocollytic, elastinolytic, and keratinolytic activity in minimal medium. These activities fell in the carbon-, nitrogen-, and phosphorous-depleted media but remained high in sulfur-depleted medium. The following model is proposed for the regulation of T. rubrum proteolytic activity. In the initial stages of infection, T. rubrum grows logarithmically. In this state, proteolytic activity is derepressed whenever carbon, nitrogen, or sulfur is lacking in the fungal milieu. The general proteinases produced would act on the nonkeratinous proteins in the stratum corneum. There are probably peptidases, as yet unidentified, that would cleave the peptides generated by the initial proteolysis into amino acids. These amino acids would provide the cell with a source of carbon, nitrogen, and sulfur. Under these conditions, the expression of general proteinases would be repressed, whereas specific keratinases would be induced in this nutrient-rich environment. Disease may occur when the fungus reaches stationary phase, when proteinases are secreted constitutively. These enzymes may directly or indirectly incite a host response, resulting in the inflammatory manifestations of dermatophytosis.
authors: Department of Pathology, University of California, San Francisco 94143.
authors: G
authors: G
authors: Apodaca
authors: J H
authors: JH
authors: McKerrow
mesh_terms: D001391:Azo Compounds / Q000494:pharmacology; D003094:Collagen / Q000494:pharmacology; D003470:Culture Media; D004549:Elastin / Q000494:pharmacology; D004794:Enzyme Repression / Q000187:drug effects; D006868:Hydrolysis; D007633:Keratins / Q000494:pharmacology; D007700:Kinetics; D010447:Peptide Hydrolases / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D010758:Phosphorus; D013455:Sulfur / Q000494:pharmacology; D014249:Trichophyton / Q000201:enzymology* / Q000254:growth & development
chemical_list: D001391:Azo Compounds; D003470:Culture Media; D010758:Phosphorus; C028470:Azocoll; D007633:Keratins; D013455:Sulfur; D003094:Collagen; D004549:Elastin; D010447:Peptide Hydrolases