Structural and immunochemical studies of the lipopolysaccharide from a new provisional serotype of Shigella flexneri.
The chemical structure of the O-antigen of a proposed new provisional serotype of Shigella flexneri has been determined. Methylation analysis, GLC-MS, 1H-NMR and 13C-NMR showed that the linear O-antigenic polysaccharide is the same as for all S. flexneri [Kenne, L., Lindberg, B., Petersson, K. & Romanowska, E. (1977) Carbohydr. Res. 56, 363-370]. A novel structural feature is that the disaccharide alpha-D-Glcp-(1----2)-alpha-D-Glcp is linked to O4 of the N-acetyl-glucosamine residue. (Formula: see text) Western blotting of the lipopolysaccharide with an E. coli R3 core-specific monoclonal antibody, suggested the presence of an E. coli R3 core.
Wehler
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Department of Organic Chemistry, Arrhenius Laboratory, University of Stockholm, Sweden.
Carlin
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D000942:Antigens, Bacterial / Q000032:analysis*; D002240:Carbohydrate Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007118:Immunoassay; D008070:Lipopolysaccharides / Q000032:analysis* / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens; D012763:Shigella flexneri / Q000276:immunology*
D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D019081:O Antigens