Analysis of hepatotoxicity in maternal and fetal rats after glucocorticoid administration by lipid histochemistry and thin layer chromatography.
Changes in lipid metabolism of fetal and maternal rat livers were investigated on day 20 of pregnancy after administration of either 3 mg/kg or 24 mg/kg triamcinolone-acetonide or 124 mg/kg hydrocortisone in crystalline suspension to the mothers on day 15 of pregnancy. Sudan black B and Nile red as well as the UV-Schiff reaction and thin layer chromatography were used to study qualitatively the response of lipids to these glucocorticoids. Generally, after application of triamcinolone-acetonide fetal livers accumulated more lipids as toxic response to this glucocorticoid than the maternal organ; the degree of lipid accumulation was clearly dose-dependent in the fetuses. After hydrocortisone treatment, lipids in maternal livers were slightly, those in the fetuses were not affected. Histochemistry and thin layer chromatography revealed an accumulation of neutral lipids, especially of triglycerides and fatty acids which both contained increased amounts of ethylene bonds after treatment with triamcinolone-acetonide. The results also show that using combined histochemistry and thin layer chromatography, the analysis of hepatic lipids is a promising tool for the assessment of toxic effects of glucocorticoids on fetal and maternal hepatocytes in rats.
Frank
H G
HG
Department of Anatomy, Free University of Berlin, Germany.
Gossrau
R
R
Graf
R
R
D000818:Animals; D002855:Chromatography, Thin Layer; D005260:Female; D005333:Fetus / Q000378:metabolism*; D005938:Glucocorticoids / Q000633:toxicity*; D006651:Histocytochemistry; D006854:Hydrocortisone / Q000633:toxicity; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008431:Maternal-Fetal Exchange; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling; D014222:Triamcinolone Acetonide / Q000633:toxicity
D005938:Glucocorticoids; D008055:Lipids; D014222:Triamcinolone Acetonide; D006854:Hydrocortisone