Expression of Drosophila Shaker potassium channels in mammalian cells infected with recombinant vaccinia virus.
A recombinant vaccinia virus containing a Drosophila potassium channel (Shaker H4) cDNA was constructed by homologous recombination between wild-type vaccinia virus DNA and a transfer plasmid. The new virus was used to infect four types of mammalian cells in culture. Electrophysiological recording 24-72 hr after infection revealed the expression of voltage-gated transient potassium channels in all four cell types. The properties of the induced currents were identical to those previously observed following injection of the Shaker H4 transcript into oocytes. Vaccinia promises to be an effective vehicle for the heterologous expression of transmembrane ion channels in a variety of cell types.
Leonard
R J
RJ
Division of Biology, California Institute of Technology, Pasadena 91125.
Karschin
A
A
Jayashree-Aiyar
S
S
Davidson
N
N
Tanouye
M A
MA
Thomas
L
L
Thomas
G
G
Lester
H A
HA
D015761:4-Aminopyridine / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D018999:Charybdotoxin; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000378:metabolism; D004330:Drosophila / Q000235:genetics*; D015870:Gene Expression; D008564:Membrane Potentials / Q000187:drug effects; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D010957:Plasmids; D015221:Potassium Channels / Q000502:physiology*; D012604:Scorpion Venoms / Q000494:pharmacology; D014616:Vaccinia virus / Q000235:genetics*
D004274:DNA, Recombinant; D008565:Membrane Proteins; D015221:Potassium Channels; D012604:Scorpion Venoms; D018999:Charybdotoxin; D015761:4-Aminopyridine