title: Nitroblue tetrazolium reduction in monocytes and monocyte-derived macrophages. Effect of oxidative burst stimulants and interferons.
abstract: The ability to mount an oxidative burst (OB) in response to medium, zymosan and phorbol myristate acetate (PMA) was assessed in human blood monocytes cultured for 1 day (MO) and monocyte-derived macrophages cultured for 10 days (MDM). Further, the effect of recombinant interferons (IFNs) on OB generation was examined. The OB was measured as a reduction of nitroblue tetrazolium (NBT). Unstimulated and stimulated NBT reduction per cell nucleus and the ratio of stimulated/unstimulated NBT reduction was not significantly different in cells cultured for 1 and 10 days. In MO, IFN-gamma stimulated the OB when co-stimulated with zymosan or PMA. IFN-alpha reduced MO adherence. When the lower adherence was corrected for, IFN-alpha enhanced NBT reduction. In MDM, a high concentration of IFN-gamma stimulated the OB without co-stimulation, in lower concentrations the presence of a co-stimulant was necessary for OB stimulation. IFN-alpha/beta enhanced the OB in response to PMA, suggesting that IFN-alpha/beta has a role in macrophage activation.
authors: Kaptein W. Wilhelmsen og Frues Institute of Bacteriology, National Hospital of Norway, University of Oslo.
authors: F
authors: F
authors: Müller
authors: H
authors: H
authors: Rollag
authors: S S
authors: SS
authors: Frøland
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D007372:Interferons / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism*; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D010084:Oxidation-Reduction; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D015054:Zymosan / Q000494:pharmacology*
chemical_list: D009580:Nitroblue Tetrazolium; D007372:Interferons; D015054:Zymosan; D013755:Tetradecanoylphorbol Acetate