Crystals of wild-type, mutated, derivatized and complexed 50 S ribosomal subunits from Bacillus stearothermophilus suitable for X-ray analysis.
Three-dimensional single crystals of wild-type and mutated 50 S ribosomal subunits from Bacillus stearothermophilus, as well as crystals of reconstituted subunits containing heavy-atom clusters and complexes of these subunits with tRNA and a short nascent polypeptide chain, were grown from polyethylene glycol in the presence of salts at low concentrations. Within experimental error, all these crystals are isomorphous, packed with monoclinic symmetry (C2) in unit cells of a = 300 A, b = 546 A, c = 377 (+/- 1%) A and beta = 112 degrees. Using synchrotron radiation at 85 to 100 K they diffract to 11 A resolution and can be irradiated for hours without disintegrating, so that a complete data set could be collected from a single crystal.
Müssig
J
J
Max-Planck-Institute for Molecular Genetics, Berlin.
Makowski
I
I
von Böhlen
K
K
Hansen
H
H
Bartels
K S
KS
Wittmann
H G
HG
Yonath
A
A
D001411:Geobacillus stearothermophilus / Q000235:genetics*; D009154:Mutation; D012329:RNA, Bacterial; D012343:RNA, Transfer; D012270:Ribosomes; D014961:X-Ray Diffraction
D012329:RNA, Bacterial; D012343:RNA, Transfer