Contribution of post-secretory mechanisms to the observed pattern of histamine and 5-hydroxytryptamine secretion from peritoneal rat mast cells in response to compound 48/80.
Compound 48/80 induces a greater percentage release of histamine than 5-HT from purified peritoneal rat mast cells such that the log-concentration effect curves diverge. Mast cells were shown to remove histamine and 5-HT applied exogenously, although uptake of 5-HT was in preference to that of histamine. At concentrations of clomipramine and fluoxetine that were shown to inhibit 5-HT uptake, the net percentage release of 5-HT increased in a corresponding manner. Likewise, pargyline and nialamide, which inhibit monoamine oxidase, potentiated the release of 5-HT, although they were without effect upon 5-HT uptake. Observed differences in the pattern of histamine and 5-HT secretion are proposed to be the result of post-secretory events involving amine reuptake and metabolism rather than a reflection of differential release mechanisms.
Purcell
W M
WM
Hatfield Polytechnic, UK.
Cohen
D L
DL
Hanahoe
T H
TH
D000639:Amitriptyline / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport; D002469:Cell Separation; D002997:Clomipramine / Q000494:pharmacology; D005260:Female; D005473:Fluoxetine / Q000494:pharmacology; D006632:Histamine / Q000378:metabolism; D006636:Histamine Release / Q000187:drug effects*; D008407:Mast Cells / Q000378:metabolism*; D009526:Nialamide / Q000494:pharmacology; D010293:Pargyline / Q000494:pharmacology; D010529:Peritoneal Cavity / Q000166:cytology; D010718:Phosphatidylserines / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012636:Secretory Rate / Q000187:drug effects; D012701:Serotonin / Q000378:metabolism*; D013696:Temperature
D010718:Phosphatidylserines; D005473:Fluoxetine; D000639:Amitriptyline; D012701:Serotonin; D006632:Histamine; D010293:Pargyline; D002997:Clomipramine; D009526:Nialamide