title: Pyrophosphate analogues in pyrophosphorolysis reaction catalyzed by DNA polymerases.
abstract: It is demonstrated here that rat liver DNA polymerase beta catalyzes the pyrophosphorolysis reaction with pyrophosphate (PPi) and its analogues. The substrate specificity of the PPi-binding site of several DNA polymerases was investigated. It was discovered that the ability of DNA polymerases to utilize PPi analogues instead of PPi in the pyrophosphorolysis reaction was markedly restricted. Only imidodiphosphate and methylenediphosphonate were demonstrated as participating in this process. Oxodiphosphonate and phosphonoformate inhibited DNA synthesis, but probably not via the interaction with the PPi-binding site of DNA polymerases.
authors: V.A. Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
authors: T
authors: T
authors: Rozovskaya
authors: N
authors: N
authors: Tarussova
authors: S
authors: S
authors: Minassian
authors: A
authors: A
authors: Atrazhev
authors: M
authors: M
authors: Kukhanova
authors: A
authors: A
authors: Krayevsky
authors: Z
authors: Z
authors: Chidgeavadze
authors: R
authors: R
authors: Beabealashvilli
mesh_terms: D000818:Animals; D004247:DNA / Q000096:biosynthesis; D004256:DNA Polymerase I / Q000378:metabolism*; D004164:Diphosphonates / Q000378:metabolism; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000037:antagonists & inhibitors / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D010710:Phosphates / Q000378:metabolism* / Q000494:pharmacology; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D051381:Rats; D013379:Substrate Specificity
chemical_list: D004164:Diphosphonates; D010710:Phosphates; C016514:imidodiphosphonic acid; C027474:methylene diphosphonate; D004247:DNA; D012194:RNA-Directed DNA Polymerase; D004256:DNA Polymerase I; D005090:Exodeoxyribonucleases; D043211:Exodeoxyribonuclease V