Effects of human interleukin-3 on granulocytic colony-forming cells in human bone marrow.
Human multilineage colony-stimulating factor (multi-CSF)/interleukin-3 (IL-3) induces colony formation from CFU-GEMM, BFU-E, and CFU-Eo when applied to in vitro cultures of highly enriched hematopoietic progenitor cells. No granulocytic colonies are formed in response to IL-3. However, with appropriate assays, we demonstrate that IL-3 increases the size of G-CSF-induced granulocytic colonies; these colonies contain greater proportions of immature cells as compared with colonies stimulated by G-CSF alone. Furthermore, IL-3 promotes the survival of CFU-G in vitro, whereas in cultures not supplemented with IL-3, CFU-G extinguish within seven days. We conclude that IL-3, although it does not stimulate granulocytic colony formation by itself, regulates the survival and proliferative rate of granulocytic progenitors.
Bot
F J
FJ
Dr Daniel den Hoed Cancer Center, Rotterdam, The Netherlands.
van Eijk
L
L
Schipper
P
P
Löwenberg
B
B
D000328:Adult; D018952:Antigens, CD34; D000943:Antigens, Differentiation; D001853:Bone Marrow / Q000187:drug effects*; D002469:Cell Separation; D002470:Cell Survival / Q000187:drug effects; D003114:Colony-Forming Units Assay; D006098:Granulocytes / Q000145:classification / Q000187:drug effects* / Q000502:physiology; D006412:Hematopoietic Stem Cells / Q000145:classification / Q000187:drug effects*; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology*; D007958:Leukocyte Count / Q000187:drug effects; D009000:Monocytes; D010641:Phenotype
D018952:Antigens, CD34; D000943:Antigens, Differentiation; D007377:Interleukin-3