Purification, cloning and nucleotide sequence determination of cynomolgus monkey apolipoprotein C-II: comparison to the human sequence.
We have purified apolipoprotein C-II (apo C-II) from cynomolgus monkey plasma, prepared antibody against it and used the antibody to isolate a cDNA containing the complete coding sequence for cynomolgus monkey apo C-II. Sequence analysis indicated that the monkey apo C-II cDNA was 200 bp longer than the human and the difference in size was all in the 5 degrees untranslated region of mRNA. This was confirmed by Northern analysis of human and monkey RNA. There was an open reading frame in the monkey apo C-II cDNA sequence encoding a preprotein of 101 amino acids - identical in size to the human protein. The carboxyl terminal 44 amino acids of the protein were 100% homologous to the human apo C-II amino acid sequence indicating evolutionary conservation of both structure and function. However, the amino terminal 35 amino acids of the protein were only 75% homologous and the amino terminal 19 amino acids were only 58% homologous to the human sequence. The amino acid sequence derived from the nucleotide sequence predicts a more basic protein than the human apo C-II and this is confirmed by isoelectric focusing and immunoblotting.
Whitted
B E
BE
Molecular Biology Research, Upjohn Company, Kalamazoo, MI 49007.
Castle
C K
CK
Polites
H G
HG
Melchior
G W
GW
Marotti
K R
KR
D000595:Amino Acid Sequence; D000818:Animals; D053304:Apolipoprotein C-II; D001056:Apolipoproteins C / Q000032:analysis / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D006801:Humans; D008252:Macaca fascicularis; D008969:Molecular Sequence Data; D012313:RNA / Q000032:analysis / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid
D053304:Apolipoprotein C-II; D001056:Apolipoproteins C; D012313:RNA; D004247:DNA