The effects of hydrocortisone on c-fos, c-myc and c-ras oncogene expression in IMR-90 fibroblasts.
The effects of hydrocortisone on oncogene expression in human IMR-90 fibroblasts was analyzed by Northern blotting of total RNA. In synchronized fibroblasts stimulated with serum alone, there were two time periods of increased c-fos expression during the G1 phase of the cell cycle. There was no significant difference between cells treated with serum plus hydrocortisone, and cells treated with serum alone with respect to c-fos expression. Quiescent cells showed no change in c-fos expression during the G1 phase of the cell cycle. Three peaks of c-fos expression occur when cells are treated with hydrocortisone alone, but hydrocortisone in the absence of serum is insufficient to initiate DNA synthesis. Hydrocortisone has no effect on c-myc or c-Ha-ras expression in the presence or absence of serum in synchronized fibroblasts. Therefore, the control of mRNA production of the nuclear oncogenes c-fos and c-myc, and the cytoplasmic oncogene c-ras are independent and hydrocortisone may enhance DNA synthesis by increasing c-fos expression.
Fritch
D F
DF
Department of Pharmacology, Thomas Jefferson University, Philadelphia, PA 19107.
Kaji
H
H
D002478:Cells, Cultured; D005347:Fibroblasts; D006133:Growth Substances; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology*; D011519:Proto-Oncogenes / Q000187:drug effects*; D012313:RNA / Q000032:analysis / Q000302:isolation & purification
D006133:Growth Substances; D012313:RNA; D006854:Hydrocortisone