Studies on the mechanism responsible for thyrotropin-induced expression of microsomal/peroxidase antigen in FRTL-5 cells.
The expression of the microsomal (M) antigen on the surface and in the cytoplasm of a strain of rat thyroid cells (FRTL-5) is under the regulation of TSH. In the present report the mechanism by which TSH induces such expression was investigated with the use of human microsomal antibody-positive serum and an indirect immunofluorescence technique. Studies were also performed to ascertain whether the M antigen of FRTL-5 cells could be identified with thyroid peroxidase (TPO), as suggested by recent data obtained in human thyroid tissue. Preabsorption experiments showed that, like solubilized human thyroid microsomes, purified human TPO completely abolished the binding of microsomal antibody to FRTL-5 cells. No inhibition was obtained by preabsorption with control human tissues (placenta, liver, and spleen) or human thyroglobulin, indicating that the antigen recognized by microsomal antibody in FRTL-5 cells was TPO. After 72 h of TSH withdrawal from the culture medium the M/TPO antigen disappeared from the surface and the cytoplasm of FRTL-5 cells. Readdition of TSH (250 microU/ml) to the culture medium of cells lacking the M/TPO antigen elicited its reappearance within 24-48 h. This effect of TSH was prevented by 10 microM cycloheximide or 0.5-5 micrograms/ml actinomycin D. Two well known stimulators of the adenylate cyclase-cAMP system, cholera toxin and forskolin, mimicked TSH in inducing the reappearance of the M/TPO antigen. A similar effect was observed with use of the phosphodiesterase inhibitor isobutylmethylxanthine. Reappearance of M/TPO antigen was also produced by the cAMP analog 8-bromo-cAMP. The tumor promoter 12-O-tetradecanoyl-phorbol 13-acetate, which stimulates thyroid cell growth through a cAMP-independent pathway, was ineffective in inducing the M/TPO antigen in FRTL-5 cells. The present data indicate that 1) thyroid peroxidase accounts for most, if not all, of the microsomal antigen of FRTL-5 cells; and 2) TSH modulates the expression of the M/TPO antigen in FRTL-5 cells by a mechanism that involves cAMP production and requires mRNA formation and subsequent protein synthesis.
Chiovato
L
L
Cattedra di Endocrinologia e Medicina Costituzionale, University of Pisa, Italy.
Vitti
P
P
Lombardi
A
A
Ceccarelli
P
P
Cucchi
P
P
Marcocci
C
C
Carayon
P
P
Pinchera
A
A
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D001324:Autoantigens / Q000096:biosynthesis*; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D003593:Cytoplasm / Q000276:immunology; D003609:Dactinomycin / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D007453:Iodide Peroxidase; D033862:Iron-Binding Proteins; D007700:Kinetics; D008861:Microsomes / Q000276:immunology; D009195:Peroxidase / Q000276:immunology*; D014176:Protein Biosynthesis; D051381:Rats; D013961:Thyroid Gland / Q000187:drug effects / Q000276:immunology*; D013967:Thyroiditis, Autoimmune / Q000276:immunology; D013972:Thyrotropin / Q000494:pharmacology*
D001324:Autoantigens; D033862:Iron-Binding Proteins; D003609:Dactinomycin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D013972:Thyrotropin; D003513:Cycloheximide; D009195:Peroxidase; C056658:TPO protein, human; D007453:Iodide Peroxidase; D015056:1-Methyl-3-isobutylxanthine