A protective monoclonal antibody that reacts with a novel antigen of pneumococcal teichoic acid.
D3114/63 is a hybridoma cell line producing a monoclonal antibody that appears to recognize a common antigenic determinant associated with the teichoic acids of Streptococcus pneumoniae. This hybridoma was produced in a fusion of splenic lymphocytes from an X-linked immunodeficient (xid) male (CBA/NxBALB/c)F1 mouse hyperimmunized with a heat-killed type 6A strain EF-3114. Treatment of EF-3114 with periodate, but not with protease, destroyed its ability to bind D3114/63. The specificity of D3114/63 for teichoic acids was suggested by the ability of both C-polysaccharide and F-antigen to inhibit its binding to EF-3114. Inhibition studies indicated that D3114/63 does not bind the PC determinant of teichoic acids. As in the case with anti-PC antibodies, the presence of an intact pneumococcal capsule significantly reduced the binding of D3114/63 to viable pneumococci. By means of a colony immunoblot procedure, it was determined that D3114/63 bound to all 97 pneumococcal strains tested including representatives of 24 different serotypes. Both D3114/63 and anti-PC antibodies were able to detect pneumococcal antigen in spinal fluids of 3 out of 4 patients with pneumococcal meningitis. D3114/63 protected mice from fatal infection with type 3 strain WU2 pneumococci but not from type 5 strain DBL5 or type 6A strain EF-3114.
McDaniel
L S
LS
Department of Microbiology, University of Alabama, Birmingham 35294.
Waltman
W D
WD
Gray
B
B
Briles
D E
DE
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D001667:Binding, Competitive; D002473:Cell Wall / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D006825:Hybridomas; D015151:Immunoblotting; D007075:Immunoglobulin M / Q000276:immunology; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D011008:Pneumococcal Infections / Q000517:prevention & control; D011135:Polysaccharides, Bacterial / Q000276:immunology; D013296:Streptococcus pneumoniae / Q000276:immunology* / Q000648:ultrastructure; D013682:Teichoic Acids / Q000276:immunology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes; D007075:Immunoglobulin M; D011135:Polysaccharides, Bacterial; D013682:Teichoic Acids