Morphometry of Rambourg-positive and Rambourg-negative beta-cell granules after culture with low and high glucose concentrations.
Dispersed islet cells from noninbred ob/ob mice were cultured for 3 days with 3 or 20 mM D-glucose and silver stained according to Rambourg et al. Two tinctorial subsets of dark and light intracellular granules were analyzed by morphometry at the ultrastructural level. The two types of granules were similar in size and shape. However, with 3 mM glucose the dark granule cores were surrounded by larger vesicles than the light granules. With 20 mM glucose, both types of granule vesicles and cores became smaller and dark-granule cores became more rounded, compared with cultures with 3 mM glucose. The higher glucose concentration also induced a marked decrease in the number (-84%) and volume density (-90%) of dark granules. In contrast, the number of light granules increased (+60%) with maintenance of their volume density. We suggest that the dark Rambourg-positive and the light Rambourg-negative beta-cell granules are functionally distinct subsets. The dark granules are probably engaged in insulin discharge. We discuss the unclear role of the light granules with a view to previously postulated heterogeneities of the insulin granule pool and their significance for exocytosis and intracellular hormone degradation.
Norlund
R
R
Department of Histology and Cell Biology, University of Umeå, Sweden.
Norlund
L
L
Täljedal
I B
IB
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured; D003594:Cytoplasmic Granules / Q000145:classification / Q000648:ultrastructure*; D005947:Glucose / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000166:cytology / Q000648:ultrastructure*; D008854:Microscopy, Electron; D009994:Osmolar Concentration; D012834:Silver; D013194:Staining and Labeling
D012834:Silver; D005947:Glucose