Inhibition of expression of SV40 virus large T-antigen by antisense oligodeoxyribonucleotides.
Expression of large T-antigen in COS cells can be inhibited by treatment of cell monolayers with oligodeoxyribonucleotides complementary to large T mRNA, which were covalently linked to poly-L-lysine. Strongest inhibition was observed with conjugates of oligodeoxynucleotides that hybridize to the sequence immediately 3' to the cap structure of the mRNA. Treatment of SV40 virus-infected CV-1 cells with the same conjugates reduces the virus-induced large T-antigen expression by more than 80%.
Westermann
P
P
Zentralinstitut für Molekularbiologie, Akademie der Wissenschaften der DDR, Berlin-Buch.
Gross
B
B
Hoinkis
G
G
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D002460:Cell Line; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D011107:Polylysine; D012313:RNA / Q000187:drug effects / Q000235:genetics*; D012315:RNA Caps / Q000235:genetics; D016372:RNA, Antisense; D012333:RNA, Messenger / Q000037:antagonists & inhibitors*; D013539:Simian virus 40 / Q000187:drug effects / Q000235:genetics*
D000952:Antigens, Polyomavirus Transforming; D009838:Oligodeoxyribonucleotides; D012315:RNA Caps; D016372:RNA, Antisense; D012333:RNA, Messenger; D011107:Polylysine; D012313:RNA