title: Recognition of rat liver and kidney nuclear T3 receptors by an antibody against c-erb A peptide.
abstract: It has been reported that c-erb A encodes nuclear T3 receptors (NT3R). Based on the sequence of c-erb A cDNA, we synthesized a polypeptide consisting of 15 amino acids, the sequence of which has high homology between c-erb A alpha 1 and beta. The antibody against this c-erb A peptide not only immunoprecipitated rat liver and kidney NT3R but also inhibited T3 binding to NT3R. In a displacement study, the inhibition of [125I]T3-binding by the antibody was parallel to that by T3 in terms of the concentration of the competitor added in the incubation mixture. Scatchard analysis revealed that the antibody decreased the value for the association constant in a dose dependent manner. The antibody did not bind T3 itself. The results show that the antibody against c-erb A peptide recognizes rat liver and kidney NT3R and that the sequence encoding this peptide, the closest carboxyl-terminal of c-erb A may be critical or at least closely related to the hormone binding.
authors: Department of Internal Medicine, Kyoto University School of Medicine, Japan.
authors: H
authors: H
authors: Nakamura
authors: T
authors: T
authors: Tagami
authors: K
authors: K
authors: Masuda
authors: T
authors: T
authors: Mitani
authors: H
authors: H
authors: Imura
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000941:Antigens / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007163:Immunosorbent Techniques; D007668:Kidney / Q000032:analysis*; D008099:Liver / Q000032:analysis*; D010446:Peptide Fragments / Q000276:immunology; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000276:immunology*; D051381:Rats; D011988:Receptors, Thyroid Hormone / Q000032:analysis / Q000276:immunology*; D014284:Triiodothyronine / Q000378:metabolism
chemical_list: D000906:Antibodies; D000941:Antigens; D000939:Epitopes; D007074:Immunoglobulin G; D010446:Peptide Fragments; D011518:Proto-Oncogene Proteins; D011988:Receptors, Thyroid Hormone; D014284:Triiodothyronine