Heterologous serum albumin as tracer for plasma volume measurements evaluated in conscious nondehydrated and 48-hour water-deprived rats.
The feasibility of using nonradioactive heterologous serum albumin measured by rocket immunoelectrophoresis as tracer for plasma volume measurements was evaluated by comparing simultaneous distribution volumes and plasma half-lives (t1/2) of human serum albumin (HSA) and Evans blue dye (T1824) in a group of conscious rats. The animals were studied under normal water intake and after a 48-h period of water deprivation. The kinetic behavior in plasma was qualitatively similar for both tracers, but T1824 distributed beyond the HSA space and had half-lives in plasma lower than those of HSA. Water deprivation induced a 14% decrease in the HSA space, from 2.7 +/- 0.1 to 2.4 +/- 0.2 ml/100 g body weight. We conclude that serum albumin spaces measured immunoelectrophoretically are a better reflection of true plasma volume than T1824 spaces and can be used advantageously in determinations of plasma volume.
Carvalho
J S
JS
Department of Medicine, VA Medical Center, Providence, Rhode Island 02908.
D000818:Animals; D001826:Body Fluids / Q000502:physiology*; D003243:Consciousness; D005070:Evans Blue / Q000378:metabolism; D005260:Female; D006207:Half-Life; D006801:Humans; D007122:Immunoelectrophoresis; D008657:Metabolic Clearance Rate; D010953:Plasma Volume; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000378:metabolism; D013997:Time Factors; D014868:Water Deprivation / Q000502:physiology*
D012709:Serum Albumin; D005070:Evans Blue