Genetic characterization of the spaA1 determinant of the surface protein antigen gene from Streptococcus sobrinus 6715.
1. The surface protein antigen-encoding gene spaA1, previously cloned from Streptococcus sobrinus 6715 (serotype g) into the plasmid pACYC184, was examined. 2. The gene product of pYA726 was identified both by the minicell method and by in vitro transcription-translation system as a protein whose mol. wt was estimated to be 140,000. 3. The spaA1 gene was localized on the 3.75 kb AvaI-BamHI fragment of the cloned DNA. 4. An internal spaA1 promoter located close to one end of the 1.25 kb AvaI fragment within the 3.75 kb AvaI-BamHI fragment initiated the transcription for the insert DNA of S. sobrinus autonomously.
Saito
S
S
Department of Biochemistry, Nihon University School of Dentistry, Matsudo, Japan.
Abiko
Y
Y
Koyanagi
T
T
Yazawa
Y
Y
Takiguchi
H
H
D001426:Bacterial Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D000939:Epitopes / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology; D015870:Gene Expression; D007163:Immunosorbent Techniques; D008562:Membrane Glycoproteins; D008970:Molecular Weight; D010957:Plasmids; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D015183:Restriction Mapping; D013291:Streptococcus / Q000235:genetics / Q000276:immunology*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D000939:Epitopes; D008562:Membrane Glycoproteins; C055764:S-layer proteins; D012321:DNA-Directed RNA Polymerases