Immunoregulatory role of Ig isotypes. II. Activation of cells that block induction of contact sensitivity responses by antibodies of IgG2a and IgG2b isotypes.
The influence that the isotype of Ag-specific antibody has on the induction of contact hypersensitivity (CS) has been investigated. Injection (i.v.) of mice with haptenated peritoneal exudate cells (PEC) pretreated with anti-hapten mAb of the IgG2a and IgG2b isotypes results in the activation of Ag-specific afferent acting Ts cells (Ts-aff). These suppressor cells are not generated when animals are injected with anti-hapten antibodies of other isotypes. The Ts-aff cells function to inhibit the generation of CS responses when injected into naive animals. Suppression is due to the induction of both Lyt-1+,2- I-J+ and Lyt-1-,2+ I-J+ T cells, both of which adhere to the lectin Vicia villosa. Attachment of both TNP and 4-ethoxymethylene-2-phenyloxazolone haptens to the same PEC, followed by treatment with an IgG2a anti-TNP antibody, generates Ts-aff cells specific for both 4-ethoxymethylene-2-phenyloxazolone and TNP. The MHC haplotype of the PEC is irrelevant, as allogeneic PEC will also induce Ts-aff cells when injected by using an identical protocol. Ts-aff cells cannot be generated in B cell-depleted mice, nor does the Ts-aff cells generated in normal mice suppress CS responses in B cell-depleted mice. These results show that Ag-antibody complexes bound on the surface of a PEC can induce potent afferent suppression in vivo. A possible general role for antibody isotypes in directing regulatory activities is discussed.
Ptak
W
W
Department of Immunology, Copernicus School of Medicine, Cracow, Poland.
Janeway
C A
CA
Flood
P M
PM
D000276:Adjuvants, Immunologic / Q000502:physiology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology; D001667:Binding, Competitive; D003877:Dermatitis, Contact / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D006183:H-2 Antigens / Q000235:genetics; D006241:Haptens / Q000276:immunology; D007074:Immunoglobulin G / Q000502:physiology*; D007132:Immunoglobulin Isotypes / Q000502:physiology*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D010529:Peritoneal Cavity / Q000166:cytology; D050378:T-Lymphocytes, Regulatory / Q000145:classification / Q000276:immunology*; D014301:Trinitrobenzenes / Q000276:immunology
D000276:Adjuvants, Immunologic; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000939:Epitopes; D006183:H-2 Antigens; D006241:Haptens; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D014301:Trinitrobenzenes