title: Receptor-mediated adherence of Campylobacter pylori to mouse Y-1 adrenal cell monolayers.
abstract: An in vitro adherence assay was developed to study the adherence of Campylobacter pylori to mammalian cells. Strains of C. pylori were isolated from individuals with gastritis. These strains possessed the fibrillar N-acetylneuraminyllactose(neuraminlactose)-binding hemagglutinin (NLBH) and were found to adhere to monolayers of mouse Y-1 adrenal cells. Adherence was rapid, prevented by pretreatment of the Y-1 cells with Clostridium perfringens neuraminidase, and blocked by the neuraminlactose-containing protein fetuin. Electron microscopy by the immunogold technique and with anti-NLBH antibody showed that NLBH was present at the sites of interaction between C. pylori and the Y-1 cells. These results indicate that the Y-1 monolayer is a valid model for receptor-specific adherence of C. pylori.
authors: Department of Medicine, Baylor College of Medicine, Houston, Texas 77030.
authors: D G
authors: DG
authors: Evans
authors: D J
authors: DJ
authors: Evans
authors: D Y
authors: DY
authors: Graham
mesh_terms: D000311:Adrenal Glands / Q000382:microbiology* / Q000648:ultrastructure; D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects; D001426:Bacterial Proteins / Q000502:physiology*; D002167:Campylobacter / Q000502:physiology* / Q000648:ultrastructure; D002460:Cell Line; D003016:Clostridium perfringens / Q000201:enzymology; D015169:Colony Count, Microbial; D046508:Culture Techniques; D007700:Kinetics; D051379:Mice; D009439:Neuraminidase / Q000494:pharmacology; D011971:Receptors, Immunologic / Q000502:physiology*; D000509:alpha-Fetoproteins / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D011971:Receptors, Immunologic; D000509:alpha-Fetoproteins; C047105:bacterial adhesin receptor; D009439:Neuraminidase