TMA-DPH a fluorescent probe of membrane dynamics in living cells. How to use it in phagocytosis.
Trimethylammonium-diphenylhexatriene (TMA-DPH), a hydrophobic fluorescent probe, has been shown in earlier studies to possess a variety of particular properties in interaction with intact living cells--specific and rapid incorporation into the plasma membrane and partition equilibrium between the membranes and the buffer. These properties offer promising applications in membrane fluidity studies and in monitoring exocytosis kinetics. Furthermore, these properties offer a method described here for quantitative monitoring of phagocytosis kinetics, by means of simple fluorescence intensity measurements. This method is original in that it evaluates only the particles which have actually been internalized by phagocytosis, and not those adsorbed on the cell surface, and that it gives quantitative information on the amount of plasma membrane involved in the process. It has been tested on mouse bone marrow macrophages.
Illinger
D
D
Département d' Immunologie, UA 491 du CNRS, Université Louis Pasteur, Strasbourg, France.
Kubina
M
M
Duportail
G
G
Poindron
P
P
Bartholeyns
J
J
Kuhry
J G
JG
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism / Q000502:physiology*; D004161:Diphenylhexatriene / Q000031:analogs & derivatives / Q000378:metabolism; D005456:Fluorescent Dyes; D008264:Macrophages / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence / Q000379:methods; D010587:Phagocytosis; D011090:Polyenes
D005456:Fluorescent Dyes; D011090:Polyenes; D004161:Diphenylhexatriene; C033628:1-(4-(trimethylamino)phenyl)-6-phenylhexa-1,3,5-triene