A new ganglioside of the lactotetraose series, GalNAc-3'-isoLM1, detected in human meconium.
A monoclonal antibody produced by immunization with cells of the human glioma cell line D-54 MG reacted with ganglioside GM2. The binding epitope of the antibody was found to be GalNAc beta 1-4(NeuAc alpha 2-3)Gal. Immunological detection of glycolipid antigens on thin-layer plates with this monoclonal antibody, DMAb-1, revealed the presence of a new ganglioside. This ganglioside, co-migrating with NeuAc alpha 2-6Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc beta 1-1Cer(6'-LM1) and GalNAc beta 1-4(NeuAc alpha 2-3)Gal beta 1-3GalNAc beta 1-4Gla beta 1-4Glc beta 1-1Cer (GalNAc-isoGM1) at chromatographic separation was isolated from human meconium. Its structure was determined by permethylation and fast atom bombardment-mass spectometry analyses. The new ganglioside was found to be a combination of the lacto and ganglio series gangliosides, and the structure found to be GalNAc beta 1-4(NeuAc alpha 2-3)Gal beta 1-3GlcNAc alpha 1-3Gal beta 1-4Glc beta 1-1Cer(GalNAc-3'-isoLM1).
Fredman
P
P
Department of Psychiatry and Neurochemistry, Gothenburg University, St. Jörgen Hospital, Hisings Backa, Sweden.
Månsson
J E
JE
Wikstrand
C J
CJ
Vrionis
F D
FD
Rynmark
B M
BM
Bigner
D D
DD
Svennerholm
L
L
D000911:Antibodies, Monoclonal; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D000939:Epitopes / Q000032:analysis; D005678:G(M2) Ganglioside / Q000302:isolation & purification; D005732:Gangliosides / Q000276:immunology / Q000302:isolation & purification*; D006017:Glycolipids / Q000276:immunology; D006801:Humans; D007231:Infant, Newborn; D013058:Mass Spectrometry; D008470:Meconium / Q000032:analysis*; D008969:Molecular Sequence Data
D000911:Antibodies, Monoclonal; D000939:Epitopes; D005732:Gangliosides; D006017:Glycolipids; C060272:GalNAc-3'-isoL(M1) ganglioside; D005678:G(M2) Ganglioside