Presence of an intron in elongator methionine-tRNA of Halobacterium volcanii.
The gene for the elongator (internal) methionine transfer RNA (tRNA(mMet)) in the archaebacterium Halobacterium volcanii contains a 75 base pair intron (intervening sequence) and lacks the 3'-terminal CCA sequence of the mature tRNA. There is a single copy of this gene in the genome and it is expressed. Northern hybridization experiments indicate that the precursor is processed to produce mature tRNA and a free intron species. A secondary structure of the transcript can be formed in which the anticodon stem of the tRNA is extended. Two symmetrically placed three-base bulge loops separated by a 4 base pair stem are present in this extension. The cleavage sites for the removal of the intron are placed between the middle and 3' residues of these loops.
Datta
P K
PK
Department of Medical Biochemistry, Southern Illinois University, Carbondale 62901.
Hawkins
L K
LK
Gupta
R
R
D001483:Base Sequence; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D006217:Halobacterium / Q000235:genetics*; D007438:Introns; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012322:RNA Precursors / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics*; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*; D012358:RNA, Transfer, Met / Q000235:genetics*; D014158:Transcription, Genetic
D015342:DNA Probes; D004269:DNA, Bacterial; D012322:RNA Precursors; D012329:RNA, Bacterial; D012345:RNA, Transfer, Amino Acid-Specific; D012358:RNA, Transfer, Met