Relative induction of molecular forms of cytochrome P-450 in gamma-hexachlorocyclohexane exposed rat liver microsomes.
The effect of gamma-hexachlorocyclohexane (HCH), (25 mg/kg body weight, i.p., administered for 4 consecutive days) on the induction of types of cytochrome P-450 in rat liver microsomes was studied. Induced proteins were characterized by "Western" blot analysis, using anticytochrome P-450 antibodies. It was observed that HCH is a "mixed-type" inducer and mediates induction of cytochrome P-450 b/e forms by several fold and of cytochrome P-450 c and d forms by nearly three fold.
Kumar
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Industrial Toxicology Research Centre, Lucknow, India.
Dwivedi
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PP
D000818:Animals; D015153:Blotting, Western; D019363:Cytochrome P-450 CYP1A1; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis* / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D001556:Hexachlorocyclohexane / Q000633:toxicity*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D010088:Oxidoreductases / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D001556:Hexachlorocyclohexane; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D019363:Cytochrome P-450 CYP1A1