title: Nature of the oxygen species generated by xanthine oxidase involved in secretory histamine release from mast cells.
abstract: The present studies were carried out to characterize the nature of reactive oxygen species generated by the xanthine-xanthine oxidase system involved in the release of histamine by noncytotoxic and cytotoxic mechanisms. To distinguish secretory release from lytic release, mast cells were loaded with 51Cr and the release of 51Cr into the incubation medium was used as a measure of cell lysis. The secretory release of histamine was not inhibited by superoxide dismutase or catalase alone. However, together these agents inhibited the release. This suggests that the combination of superoxide and hydrogen peroxide can evoke secretory release. The lytic release of histamine, as monitored by concomitant release of 51Cr from mast cells at higher concentration of xanthine oxidase or longer periods of incubation, seems to be related to hydrogen peroxide production since catalase inhibited the cell lysis. Since it has been reported that exogenously added hydrogen peroxide at concentrations below 10 mM did not induce cell lysis, the lytic release, although hydrogen peroxide dependent, may not be due to its direct effect on the cell surface. The cell lysis observed in the xanthine-xanthine oxidase system seems to be brought about by a complex mechanism involving the interactions of hydrogen peroxide and superoxide with cellular components. These studies indicate that the beneficial effects of superoxide dismutase seen in biological systems may partly be due to inhibition of the secretory processes stimulated by superoxide.
authors: Department of Medicine and Ophthalmology, University of Toronto, Ont., Canada.
authors: I A
authors: IA
authors: Menon
authors: S
authors: S
authors: Shirwadkar
authors: N S
authors: NS
authors: Ranadive
mesh_terms: D000818:Animals; D002374:Catalase / Q000494:pharmacology; D006636:Histamine Release; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D010529:Peritoneal Cavity / Q000166:cytology*; D051381:Rats; D011919:Rats, Inbred Strains; D014969:Xanthine Oxidase / Q000378:metabolism*
chemical_list: D002374:Catalase; D014969:Xanthine Oxidase; D010100:Oxygen