Separation and visualization of apolipoprotein B species by sodium dodecyl sulfate-agarose gel electrophoresis and immunoblotting.
A method for separation and visualization of the different apolipoprotein B species using 0.2% sodium dodecyl sulfate-1.5% agarose gel electrophoresis and immunoblotting is described. The method is capable of demonstrating the different forms of apolipoprotein B (apo B) in plasma volumes of 10-50 microliters without prior ultracentrifugation. After ultracentrifugation of samples, estimation of the ratio between apo B 48 and apo B 100 is possible by scanning of Coomassie-stained gels or immunoblots.
Myrseth
L E
LE
Research Institute for Internal Medicine, University of Oslo, Norway.
Hagve
T A
TA
Prydz
H
H
D001055:Apolipoproteins B / Q000097:blood / Q000302:isolation & purification*; D003720:Densitometry; D004492:Edetic Acid; D004587:Electrophoresis, Agar Gel / Q000379:methods; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D012967:Sodium Dodecyl Sulfate; D013194:Staining and Labeling; D014461:Ultracentrifugation
D001055:Apolipoproteins B; D012967:Sodium Dodecyl Sulfate; D004492:Edetic Acid