Copper iodide staining of protein blots on nitrocellulose membranes.
Copper iodide staining which can detect protein levels as low as 100-150 pg/mm2 on nitrocellulose membranes is described. The staining is quantitative as measured by densitometry. Staining is complete within 5 min and may be removed by washing the membrane for 15 min without loss of immunoreactivity. The stain utilizes a reddish-brown precipitate of copper iodide in highly alkaline conditions. Because of its high sensitivity, convenience, and low cost, this stain may be more practical than amido black or gold- and silver-based stains for most laboratory purposes.
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Molecular Biology Institute, University of California, Los Angeles 90024.
Reisler
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D003101:Collodion; D003300:Copper; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D007150:Immunohistochemistry; D007454:Iodides; D008567:Membranes, Artificial; D011506:Proteins / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis; D013194:Staining and Labeling
D007454:Iodides; D008567:Membranes, Artificial; D011506:Proteins; D012710:Serum Albumin, Bovine; D003300:Copper; D003101:Collodion