A stain for iron-containing proteins sensitive to nanogram levels of iron.
A method for staining iron proteins on polyacrylamide gel electropherograms is described. It is based upon catalysis, by iron, of the oxidation of diaminobenzoic acid by hydrogen peroxide. Bands containing as little as 5 ng of protein-bound iron can be visualized by this method.
Kuo
C F
CF
Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710.
Fridovich
I
I
D062365:Aminobenzoates; D004591:Electrophoresis, Polyacrylamide Gel; D005288:Ferredoxins / Q000032:analysis; D006861:Hydrogen Peroxide; D007501:Iron / Q000032:analysis* / Q000494:pharmacology; D008832:Microchemistry; D010084:Oxidation-Reduction; D011506:Proteins / Q000032:analysis*; D013194:Staining and Labeling
D062365:Aminobenzoates; D005288:Ferredoxins; D011506:Proteins; C017856:3,5-diaminobenzoic acid; D006861:Hydrogen Peroxide; D007501:Iron