Identity of Leu-19 (CD56) leukocyte differentiation antigen and neural cell adhesion molecule.
Neural cell adhesion molecule (N-CAM) is a membrane glycoprotein expressed on neural and muscle tissues that is involved in homotypic adhesive interactions. We have demonstrated that N-CAM also is expressed on hematopoietic cells, and is recognized by the anti-Leu-19 mAb. Leu-19 is preferentially expressed on NK cells and T lymphocytes that mediate MHC-unrestricted cytotoxicity, but is also present on some myeloid leukemia cell lines. On NK cells, T cells, the KG1a.5 hematopoietic cell line, and a neuroblastoma cell line, Leu-19 is a approximately 140-kD polypeptide with N-linked carbohydrates and abundant sialic acid residues. Sequential immunoprecipitation and peptide mapping demonstrated that the Leu-19 and N-CAM molecules expressed on leukocyte and neuroblastoma cell lines are similar structures. These findings suggest that the Leu-19 antigen on leukocytes may be involved in cell adhesion, analogous to the function on N-CAM on neural cells.
Lanier
L L
LL
Becton Dickinson Monoclonal Center, Inc., Mountain View, California 94043.
Testi
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Bindl
J
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Phillips
J H
JH
D000945:Antigens, Differentiation, T-Lymphocyte / Q000235:genetics / Q000302:isolation & purification*; D000954:Antigens, Surface / Q000235:genetics / Q000302:isolation & purification*; D019002:CD56 Antigen; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules; D002450:Cell Communication; D002460:Cell Line; D006412:Hematopoietic Stem Cells / Q000032:analysis; D006801:Humans; D007694:Killer Cells, Natural / Q000032:analysis; D007951:Leukemia, Myeloid / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics / Q000302:isolation & purification*; D019158:N-Acetylneuraminic Acid; D009417:Nerve Tissue / Q000032:analysis; D012794:Sialic Acids / Q000032:analysis; D013601:T-Lymphocytes / Q000032:analysis; D014158:Transcription, Genetic
D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D019002:CD56 Antigen; D015815:Cell Adhesion Molecules; D008562:Membrane Glycoproteins; D012794:Sialic Acids; D019158:N-Acetylneuraminic Acid