Differential immunostaining of oncocytic renal tumors with an anti-renal cell carcinoma monoclonal antibody.
Formalin-fixed, paraffin embedded tissue sections from twenty three oncocytic renal neoplasms were stained with hematoxylin and eosin, retrospectively examined, and graded according to the criteria reported by Lieber and associates. Additional sections were stained by the avidin-biotin immunoperoxidase technique with anti-renal cell carcinoma monoclonal antibody 5F4. The results showed that cytological heterogeneity was the most prominent feature of the tumors. Four cases were composed predominantly of grade 1 cells, but also had foci of grade 2 cells. Seventeen cases were composed predominantly of grade 2 cells. Two cases were composed predominantly of grade 3 cells. Immunostaining with 5F4 showed differential reactivity between grade 1 and grades 2 and 3 cells. The antibody highlighted the foci of atypical cells which were difficult to detect by routine hematoxylin and eosin staining and thus could be useful in the differential diagnosis of oncocytic renal neoplasms.
Kochevar
J
J
Dept. of Pathology and Laboratory Medicine, College of Medicine, Texas A & M University, College Station 77843.
Coffield
K S
KS
D000236:Adenoma / Q000175:diagnosis* / Q000473:pathology; D000911:Antibodies, Monoclonal; D002292:Carcinoma, Renal Cell / Q000175:diagnosis* / Q000473:pathology; D003937:Diagnosis, Differential; D006801:Humans; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000473:pathology; D007680:Kidney Neoplasms / Q000175:diagnosis* / Q000473:pathology; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal