Synthesis of a more stable osmium ammine electron-dense DNA stain.
Specific DNA staining for electron microscopic observation is simplified by a shorter synthesis of the staining reagent. The new, more reliable reagent, osmium ammine-B, is stable for more than a year, dissolves completely in water, and does not require reoptimization of staining conditions for every batch, yet reproducibly gives strong contrast to DNA-containing structures.
Olins
A L
AL
University of Tennessee-Oak Ridge, Graduate School of Biomedical Sciences.
Moyer
B A
BA
Kim
S H
SH
Allison
D P
DP
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D004247:DNA / Q000032:analysis*; D004355:Drug Stability; D008099:Liver / Q000032:analysis; D008854:Microscopy, Electron; D009992:Osmium / Q000138:chemical synthesis*; D017892:Osmium Compounds; D000644:Quaternary Ammonium Compounds / Q000138:chemical synthesis*; D051381:Rats; D012995:Solubility; D013194:Staining and Labeling; D014867:Water
D017892:Osmium Compounds; D000644:Quaternary Ammonium Compounds; C058639:osmium ammine B; D014867:Water; D009992:Osmium; D004247:DNA