Human osteoblast-like cells expressing MHC class II determinants stimulate allogeneic and autologous peripheral blood mononuclear cells and function as antigen-presenting cells.
We have previously shown that a subset of human osteoblast-like bone surface cells (HBC) derived from explants of trabecular bone constitutively express class II major histocompatibility complex (MHC) determinants in long-term culture. We now examine the capacity of HBC to stimulate allogeneic and autologous peripheral blood mononuclear cells (PBMC) and to function as antigen-presenting cells (APC). HBC consistently stimulated a strong proliferative response in allogeneic PBMC after 6 days co-culture. Stimulation of autologous PBMC was weaker and less consistent. HBC and adherent PBMC were equally active in stimulating a PPD response in autologous non-adherent PBMC. These observations suggest that immune cells initiated by HBC may play a role in normal and disordered bone remodelling processes.
Skjødt
H
H
Department of Medicine, Sundby Hospital, Copenhagen, Denmark.
Møller
T
T
Freiesleben
S F
SF
D000328:Adult; D000368:Aged; D000938:Antigen-Presenting Cells / Q000502:physiology*; D002450:Cell Communication; D000939:Epitopes / Q000032:analysis*; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology*; D008875:Middle Aged; D010006:Osteoblasts / Q000276:immunology* / Q000502:physiology
D000939:Epitopes; D000949:Histocompatibility Antigens Class II