[Immunohistochemical analysis of 42 renal cell carcinomas and one oncocytoma with mono- and polyclonal antibodies against vimentin and cytokeratin].
42 renal cell carcinomas and 1 oncocytoma were investigated by means of immunofluorescence (including double immunofluorescence) using a panel of mono- and polyclonal antibodies to vimentin and cytokeratins. In all tumors except chromophobe cell renal carcinoma (CCRC) and oncocytoma generally a coexpression of vimentin and cytokeratins could be demonstrated; however, the intermediate filament expression was often very heterogeneous with regard to the distribution of vimentin and cytokeratins in general, depending on the mono- and polyclonality of the antibodies and on the areas of a tumor investigated. In CCRC and oncocytoma all tumor cells contained cytokeratin filaments. In addition, as revealed by double immunofluorescence, in only occasional tumor cells we could demonstrate vimentin.
Beham
A
A
Ratschek
M
M
Zatloukal
K
K
Denk
H
H
Lackinger
E
E
D000230:Adenocarcinoma / Q000378:metabolism / Q000648:ultrastructure*; D000911:Antibodies, Monoclonal; D002292:Carcinoma, Renal Cell / Q000378:metabolism / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007382:Intermediate Filaments / Q000648:ultrastructure; D007633:Keratins / Q000378:metabolism*; D007680:Kidney Neoplasms / Q000378:metabolism / Q000648:ultrastructure*; D014746:Vimentin / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D014746:Vimentin; D007633:Keratins
Immunhistochemische Untersuchungen von 42 Nierenzellkarzinomen und einem Onkozytom mit mono- und polyklonalen Antikörpern gegen Vimentin und Zytokeratine.