title: Cytotoxic drug sensitivity of squamous cell carcinoma as predicted by an in vitro testing model.
abstract: An in vitro technique for testing the chemosensitivity of squamous cell carcinoma from the head and neck is described. Single-cell suspensions and tumor slices obtained from human xenografts were used. The sensitivity to cisplatin and bleomycin was quantified using the incorporation of (3H) thymidine as a parameter. The in vitro assays were compared with in vivo tests in mice. No reliable data could be obtained with cell suspensions of these tumors due to a rapid decrease in cell viability during incubation, while tumor slices revealed a stable control level of (3H) thymidine incorporation for up to 30 hours. In 6 out of 7 cases close agreement was found between the effect of the cytotoxic drug on the tissue slices and on the same tumors in nude mice.
authors: Department of Otorhinolaryngology, University of Nijmegen, The Netherlands.
authors: D
authors: D
authors: Elprana
authors: J
authors: J
authors: Schwachöfer
authors: W
authors: W
authors: Kuijpers
authors: P
authors: P
authors: Van Den Broek
authors: D J
authors: DJ
authors: Wagener
mesh_terms: D000818:Animals; D001761:Bleomycin / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002945:Cisplatin / Q000494:pharmacology*; D006258:Head and Neck Neoplasms / Q000473:pathology*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D013936:Thymidine / Q000378:metabolism; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects*
chemical_list: D001761:Bleomycin; D002945:Cisplatin; D013936:Thymidine