A simple method for determination of the concentration of anti-dextran IgG in antiserum by means of affinity electrophoresis.
A simple technique for determination of the concentration of anti-dextran IgG in antiserum using affinity electrophoresis is described. In the presence of an excess of intermediary molecular size dextran in the polyacrylamide gel, polyclonal anti-dextran IgG migrated in a single sharp band, separated from the nonspecific IgG fraction and other serum protein fractions. With this technique, 1-10 micrograms anti-dextran IgG in antisera can be determined within 3 h. We call the procedure ligand saturating affinity electrophoresis.
Tanaka
T
T
Department of Biochemistry, Yamaguchi University School of Medicine, Ube, Japan.
Nakamura
K
K
Takeo
K
K
D000818:Animals; D003911:Dextrans / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D007106:Immune Sera / Q000032:analysis; D007114:Immunization; D007074:Immunoglobulin G / Q000032:analysis* / Q000378:metabolism; D008024:Ligands; D011817:Rabbits
D003911:Dextrans; D007106:Immune Sera; D007074:Immunoglobulin G; D008024:Ligands