Localization of substance P and neurofilament immunoreactive fibers in the lumbar facet joint capsule and supraspinous ligament of the rabbit.
An indirect immunofluorescence method was utilized to identify substance P-like immunoreactive (SPLI) and neurofilament protein immunoreactive (NFIR) fibers in the lumbar facet joint capsule and supraspinous ligament of the rabbit. The results demonstrated a large population of NFIR fibers, indicating that these tissues are richly innervated, and a smaller population of SPLI fibers. In some fibers, neurofilament protein and substance P were colocalized. The data suggest that the facet joint capsule and the supraspinous ligament contain SPLI nociceptive fibers that could be a source of low back pain.
el-Bohy
A
A
Department of Anatomy and Cell Biology, School of Medicine, Wayne State University, Detroit, MI 48201.
Cavanaugh
J M
JM
Getchell
M L
ML
Bulas
T
T
Getchell
T V
TV
King
A I
AI
D000818:Animals; D000911:Antibodies, Monoclonal; D003599:Cytoskeleton / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D007381:Intermediate Filament Proteins / Q000032:analysis* / Q000276:immunology; D007382:Intermediate Filaments / Q000648:ultrastructure*; D007596:Joints / Q000166:cytology*; D008022:Ligaments / Q000166:cytology; D016900:Neurofilament Proteins; D011817:Rabbits; D013131:Spine / Q000166:cytology; D013373:Substance P / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins; D013373:Substance P