Effect of interleukin 1 beta on transducing mechanisms in 235-1 clonal pituitary cells. Part II: Modulation of calcium fluxes.
In the present study we investigated the effect of the interleukin 1 beta on intracellular free calcium concentrations in 235-1 cell line both in basal conditions and after stimulation by the calcium channel activator maitotoxin. Interleukin 1 beta (from 0.01 pM to 10 nM) was unable to significantly affect basal cytosolic free calcium levels in acute conditions. The preincubation of these cells with interleukin 1 beta for 48h modulates maitotoxin stimulation of calcium fluxes without modifying basal intracellular free calcium levels. Low concentrations of interleukin 1 beta (0.01 pM, 1 pM) caused a marked reduction of intracellular free calcium concentrations increase induced by maitotoxin while higher doses of the monokine potentiated maitotoxin stimulation of calcium fluxes. The specificity of interleukin 1 beta effect was tested by means of polyclonal anti-interleukin 1 beta antibody (titer 1:100) which significantly abolished the inhibitory effect of interleukin 1 beta on free cytosolic calcium levels. These results show that a long lasting interaction of interleukin 1 beta with its receptor is able to influence voltage-sensitive calcium channels activation induced by maitotoxin in 235-1 cells.
Schettini
G
G
Dept. of Pharmacology, II School of Medicine, Napoli, Italy.
Meucci
O
O
Florio
T
T
Scala
G
G
Landolfi
E
E
Grimaldi
M
M
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002999:Clone Cells; D004357:Drug Synergism; D007375:Interleukin-1 / Q000494:pharmacology*; D007473:Ion Channels / Q000187:drug effects* / Q000378:metabolism; D008387:Marine Toxins / Q000494:pharmacology; D035964:Oxocins; D010902:Pituitary Gland / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D000095702:Polyether Toxins
D002118:Calcium; D007375:Interleukin-1; D007473:Ion Channels; D008387:Marine Toxins; D035964:Oxocins; C020394:maitotoxin; D000095702:Polyether Toxins