Effect of epidermal growth factor on the sheet formation of the human keratinocyte in cell culture.
Epidermal growth factor is an important element in maintaining keratinocyte proliferation and maturation. To evaluate its effect on keratinocyte growth in vitro, human foreskins were cultured. The epidermal keratinocyte growth in culture was separated into two stages by a conditional medium: the proliferation stage, in which the cells were maintained in a monolayer; and the differentiation stage, in which the cells grew to stratification and keratinization. The keratinocytes were cultured in various concentrations of epidermal growth factor, and their morphology and growth behavior were closely observed. Our results demonstrated that cultured keratinocytes grew in a confluent layer under the influence of epidermal growth factor. In contrast, in a culture without epidermal growth factor, the proliferation rate of cultured keratinocytes slowed down and eventually the cells stopped growing. During serum stimulation, with or without additional exogenous epidermal growth factor, the cultured keratinocytes grew continuously to the normal terminal differentiation. Under this two-stage culture model, the cultured keratinocytes could grow into an intact sheet of graftable epidermis.
Lin
H C
HC
Department of Surgery, Tri-Service General Hospital, Taipei, Taiwan, Republic of China.
Piing
J T
JT
Tseng
Y M
YM
Chao
C F
CF
D000911:Antibodies, Monoclonal; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D000078404:Epidermal Cells; D004815:Epidermal Growth Factor / Q000502:physiology*; D004817:Epidermis / Q000502:physiology* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006801:Humans; D007633:Keratins; D007700:Kinetics; D008854:Microscopy, Electron
D000911:Antibodies, Monoclonal; D003470:Culture Media; D004815:Epidermal Growth Factor; D007633:Keratins