Unsuitability of laser nephelometer in the evaluation of factor VIII antigen.
We developed assays for the measurement of Factor VIII in a Laser Nephelometer and the results were compared with those obtained by conventional immunological methods. Marked discrepancies were obtained using different antisera and only the Dako antiserum lot 42 B yielded satisfactory results. Other antisera used (other lots of Dako antiserum, Behringwerke antiserum and Immunoscientific antiserum) failed to yield satisfactory results. From our data it seems that Laser Nephelometer has no role in the evaluation of Factor VIII complex. Statements to the contrary have to be accepted with great caution.
Ruzza
G
G
Institute of Medical Semeiotics, University of Padua Medical School, Italy.
Viero
M
M
Lazzaro
A R
AR
Girolami
A
A
D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000032:analysis*; D001797:Blood Protein Electrophoresis; D005169:Factor VIII / Q000032:analysis / Q000276:immunology*; D006467:Hemophilia A / Q000097:blood; D006801:Humans; D005779:Immunodiffusion; D007834:Lasers; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation*; D014842:von Willebrand Diseases / Q000097:blood; D014841:von Willebrand Factor
D000941:Antigens; D014841:von Willebrand Factor; D005169:Factor VIII