Stimulatory effect of CD5 antibody on B cells from patients with rheumatoid arthritis.
In order to clarify the role of CD5 antigen on B cell in autoimmunity, we examined B cells from patients with rheumatoid arthritis (RA). The percentages of CD5 positive B cells were increased in peripheral blood from RA compared with normal. Normal and RA B cells were stimulated with two kinds of monoclonal antibodies to CD5 (Leu-1, SL-1) which recognize different epitopes. RA B cells proliferated and secreted IgM by CD5 antibody stimulation in combination with IL-1. Our observations imply that CD5 positive B cells in RA are in their differentiation stage and that CD5 antigen might be one of the triggers to activate CD5 positive B cells in vivo to produce autoantibody.
Hara
M
M
First Department of Internal Medicine, National Defense Medical College, Saitama, Japan.
Kitani
A
A
Hirose
T
T
Norioka
K
K
Harigai
M
M
Suzuki
K
K
Tabata
H
H
Kawakami
M
M
Kawagoe
M
M
Nakamura
H
H
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000328:Adult; D000368:Aged; D000943:Antigens, Differentiation / Q000276:immunology*; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology* / Q000473:pathology; D001402:B-Lymphocytes / Q000145:classification / Q000276:immunology* / Q000378:metabolism; D018956:CD5 Antigens; D002454:Cell Differentiation; D002469:Cell Separation; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology*; D007075:Immunoglobulin M / Q000096:biosynthesis; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D010641:Phenotype
D000276:Adjuvants, Immunologic; D000943:Antigens, Differentiation; D018956:CD5 Antigens; D007106:Immune Sera; D007075:Immunoglobulin M