A marker epitope of attenuated Plasmodium berghei.
Plasmodium berghei XAT is an irradiation-induced, permanent attenuated derivative from high-virulence P. berghei NK65. Monoclonal antibodies against XAT were developed. By immunofluorescence screening, one monoclonal antibody was identified that was reactive with XAT at the schizont stage but not with NK65 nor with any other stage of intra-erythrocytic development of either parasite. The monoclonal antibody precipitated a 240-kD molecule from metabolically labeled XAT antigens. This molecule was thought to be a marker epitope of the attenuated parasite.
Takagi
T
T
Department of Parasitology, Gunma University School of Medicine, Maebashi, Japan.
Waki
S
S
Kogure
S
S
Sugioka
Y
Y
Suzuki
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000953:Antigens, Protozoan / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D005260:Female; D005455:Fluorescent Antibody Technique; D006825:Hybridomas; D007118:Immunoassay; D005779:Immunodiffusion; D051379:Mice; D008807:Mice, Inbred BALB C; D010962:Plasmodium berghei / Q000276:immunology* / Q000472:pathogenicity / Q000528:radiation effects; D014774:Virulence
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D000939:Epitopes