Effect of different oxygen concentrations in the gas phase on biochemical activities of goat mammary tissue in organ culture.
Non-secretory mammary explants from virgin goats showed higher RNA and protein synthesis in a low O2 gas phase (air) than in high O2 (95% O2). Lipid and casein synthesis was not affected significantly by the concentration of O2 in the atmosphere during culture. On the other hand, the more developed mammary tissue from primigravid goats showed higher lipid, casein and protein synthesis in 95% O2. The relative response of mammary tissue to hormones was not substantially different when cultured in the presence of a low or high O2 gas phase. As Hepes-buffered medium was found not to need a supply of CO2 to maintain the correct pH and as Hepes did not interfere with biochemical activities of cells, it is recommended to use it for cultures in a low O2 gas phase.
Skarda
J
J
Institute of Animal Physiology and Genetics, Czechoslovak Academy of Sciences, Prague.
Urbanová
E
E
D000818:Animals; D002364:Caseins / Q000096:biosynthesis; D005260:Female; D006041:Goats / Q000378:metabolism*; D008055:Lipids / Q000096:biosynthesis*; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000378:metabolism*; D009924:Organ Culture Techniques; D010100:Oxygen / Q000494:pharmacology*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000378:metabolism*; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*
D002364:Caseins; D008055:Lipids; D012313:RNA; D010100:Oxygen