Sequences of the cell-attachment sites of reovirus type 3 and its anti-idiotypic/antireceptor antibody: modeling of their three-dimensional structures.
Previous studies have identified an area of amino acid sequence similarity shared by the reovirus type 3 cell-attachment protein sigma 1 and an anti-idiotypic/antireceptor monoclonal antibody (mAb) 87.92.6 that mimics reovirus type 3 by attaching to the same cell-surface receptor. We found that synthetic peptides corresponding to this area of primary sequence similarity bind a neutralizing mAb 9BG5 against which the mAb 87.92.6 is directed. The synthetic peptides compete with mAb 87.92.6 and reovirus type 3 for binding by mAb 9BG5 and displace mAb 87.92.6 and reovirus type 3 from binding to the cell-surface reovirus type 3 receptor. Such observations show that the shared primary structure between reovirus type 3 sigma 1 polypeptide and antireceptor mAb 87.92.6 defines the oligopeptide neutralizing/cell-attachment epitope of reovirus type 3. Computer modeling of this epitope, by use of sequence similarities of known immunoglobulin hypervariable loop conformations, permits an examination of the rudimentary three-dimensional structure of this epitope.
Williams
W V
WV
Department of Pathology (Division of Immunobiology), University of Pennsylvania, Philadelphia.
Guy
H R
HR
Rubin
D H
DH
Robey
F
F
Myers
J N
JN
Kieber-Emmons
T
T
Weiner
D B
DB
Greene
M I
MI
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000936:Antigen-Antibody Complex; D002460:Cell Line; D000939:Epitopes / Q000032:analysis; D006389:Hemagglutinins, Viral / Q000235:genetics; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D007739:L Cells / Q000382:microbiology; D012089:Mammalian orthoreovirus 3 / Q000502:physiology*; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D011487:Protein Conformation; D011991:Receptors, Virus / Q000235:genetics* / Q000276:immunology; D012087:Reoviridae / Q000502:physiology*
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000939:Epitopes; D006389:Hemagglutinins, Viral; D007130:Immunoglobulin Idiotypes; D011991:Receptors, Virus; C046176:reovirus type 3 receptor