Isolation and characterization of HTLV-I from symptomatic family members with tropical spastic paraparesis (HTLV-I encephalomyeloneuropathy).
Human T lymphotropic virus type I (HTLV-I) was isolated from peripheral blood- and cerebrospinal fluid-derived mononuclear cells of a 13-y-old boy and from the peripheral blood lymphocytes of both his parents. All three had IgG antibodies to HTLV-I and varying degrees of the clinical features of tropical spastic paraparesis (TSP). The son also had IgM antibodies specific for HTLV-I in his serum. Isolations were successfully made from peripheral blood lymphocytes and cerebrospinal fluid lymphocytes stimulated with interleukin-2 or cocultivated with umbilical cord blood mononuclear cells. Established cell lines contained HTLV-I antigen by immunfluorescence and cell-associated virus by electron microscopy; cells became transformed in vitro as determined by their continuous growth in the absence of exogenous interleukin-2. This boy is the youngest TSP patient known to be reported, and the isolation of HTLV-I from all three family members suggests the causative role of this virus in TSP.
McKhann
G
G
Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, Bethesda, Maryland.
Gibbs
C J
CJ
Mora
C A
CA
Rodgers-Johnson
P E
PE
Liberski
P P
PP
Gdula
W J
WJ
Zaninovic
V
V
D000328:Adult; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D005190:Family; D005260:Female; D005312:Fetal Blood; D005455:Fluorescent Antibody Technique; D015486:HTLV-I Antigens / Q000032:analysis; D015368:Human T-lymphotropic virus 1 / Q000302:isolation & purification* / Q000648:ultrastructure; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D015493:Paraparesis, Tropical Spastic / Q000382:microbiology / Q000635:transmission*; D011247:Pregnancy; D012194:RNA-Directed DNA Polymerase / Q000032:analysis
D015486:HTLV-I Antigens; D007376:Interleukin-2; D012194:RNA-Directed DNA Polymerase