The LTR, v-src, LTR provirus generated in the mammalian genome by src mRNA reverse transcription and integration.
Different types of altered proviruses of Rous sarcoma virus (RSV) have been detected in mammalian tumor cell lines. We cloned and sequenced one of these altered proviruses with the structure LTR, v-src, LTR. The presence of an intact viral splice junction, as well as duplications of the chromosomal sequence GCGGGG flanking the two 2-base-pair-deleted LTRs, demonstrated reverse transcription and normal retroviral integration of src mRNA in mammalian cells. In addition, a 1-nucleotide deletion 2 bases upstream from the AAUAAA polyadenylation signal is suspected to be responsible for the absence of a poly(A) track in the src mRNA present in virions of rescued viruses.
Bodor
J
J
Department of Cellular and Viral Genetics, Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.
Svoboda
J
J
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics* / Q000302:isolation & purification; D006224:Cricetinae; D005814:Genes, Viral; D009374:Neoplasms, Experimental / Q000382:microbiology; D015688:Oncogene Protein pp60(v-src); D009857:Oncogenes; D011533:Proviruses / Q000235:genetics* / Q000302:isolation & purification; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D012191:Retroviridae Proteins / Q000235:genetics*; D014407:Tumor Cells, Cultured / Q000032:analysis; D014764:Viral Proteins / Q000378:metabolism
D012191:Retroviridae Proteins; D014764:Viral Proteins; D015688:Oncogene Protein pp60(v-src); D012194:RNA-Directed DNA Polymerase