Comparison between isoelectric focusing methods for the detection of orosomucoid phenotypes.
Orosomucoid (ORM) polymorphism was investigated by different methods including isoelectric focusing in acid pH ranges followed by silver staining, print immunofixation of desialyzed ORM, fixation using a lectin from the sea-weed Codium tomentosum, isoelectric focusing followed by immunofixation in miniaturized gels and isoelectric focusing in immobilized pH gradients. Population genetics studies were carried out in Galicia (NW Spain) and two new ORM variants were found.
Montiel
M D
MD
Department of Legal Medicine, University of Santiago de Compostela, Galicia, Spain.
Carracedo
A
A
Lopez-Rodriguez
I
I
Rodriguez-Calvo
M S
MS
Concheiro
L
L
Huguet
E
E
Gené
M
M
D000483:Alleles; D000665:Ampholyte Mixtures; D005782:Gels; D006801:Humans; D007120:Immunochemistry; D007525:Isoelectric Focusing / Q000379:methods*; D037102:Lectins; D009439:Neuraminidase / Q000494:pharmacology; D009961:Orosomucoid / Q000235:genetics*; D010641:Phenotype; D012834:Silver; D013030:Spain; D013194:Staining and Labeling; D009626:Terminology as Topic
D000665:Ampholyte Mixtures; D005782:Gels; D037102:Lectins; D009961:Orosomucoid; D012834:Silver; D009439:Neuraminidase