Interferon alters intracellular transport of vesicular stomatitis virus glycoprotein.
Double-label immunofluorescence staining studies in virus-infected subclone 11 of LB cells indicated that almost all of the vesicular stomatitis virus (VSV) glycoprotein (G) was plasma membrane-associated during the logarithmic phase of virus replication. In contrast, treatment with interferon (IFN) resulted in inhibition of VSV-G transport, so that almost all of the G remained associated with the Golgi complex (GC) at comparable times after infection. In both IFN-treated and control cells, G was resistant to treatment with the enzyme endo-beta-N-acetylglucosamine H (endo H) indicating that the bulk of the G had reached the trans compartment of the GC.
Singh
V K
VK
Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD.
Maheshwari
R K
RK
Damewood
G P
GP
Stephensen
C B
CB
Oliver
C
C
Friedman
R M
RM
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D006056:Golgi Apparatus / Q000378:metabolism; D006596:Hexosaminidases / Q000494:pharmacology; D007372:Interferons / Q000494:pharmacology*; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects* / Q000378:metabolism; D014763:Viral Matrix Proteins / Q000378:metabolism*; D014909:Wheat Germ Agglutinins
D008562:Membrane Glycoproteins; D014763:Viral Matrix Proteins; D014909:Wheat Germ Agglutinins; D007372:Interferons; D006596:Hexosaminidases; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase