Transcripts from the frameshifted MURF3 gene from Crithidia fasciculata are edited by U insertion at multiple sites.
In trypanosome mitochondria an RNA editing process is operative, which co- or post-transcriptionally alters the nucleotide sequence of transcripts by insertion and/or deletion of U residues at specific sites. To increase our understanding of the mechanism of this process we have compared the nucleotide sequence of the frameshifted mitochondrial MURF3 gene from Crithidia fasciculata to that of a large number of MURF3 cDNAs. We found cDNAs derived from transcripts edited at two different sites in the protein coding sequence: (i) at the frameshift position five extra U residues connect the two reading frames and (ii) at the 5' terminus 22 inserted Us shift a putative initiator codon out of phase. The collection also contained cDNAs that were derived from non-edited transcripts. Partially edited sequences were not found, except in one cDNA, which contained an edited frameshift site in combination with a non-edited 5' terminus. The analysis further showed that MURF3 transcripts have a 3'-terminal poly(AU) extension, which varies in sequence. The implications of these results are discussed.
van der Spek
H
H
Laboratory of Biochemistry, University of Amsterdam, The Netherlands.
van den Burg
J
J
Croiset
A
A
van den Broek
M
M
Sloof
P
P
Benne
R
R
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003421:Crithidia / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000235:genetics*; D000077278:RNA, Mitochondrial; D014158:Transcription, Genetic; D014529:Uridine / Q000378:metabolism*
D004272:DNA, Mitochondrial; D000077278:RNA, Mitochondrial; D012313:RNA; D014529:Uridine