Organization of the biosynthesis genes for the peptide antibiotic gramicidin S.
A recombinant bacteriophage containing the intact Bacillus brevis gene for gramicidin S synthetase 1, grsA, and the 5' end of the gramicidin S synthetase 2 gene, grsB, was identified by screening an EMBL3 library with anti-GrsA antibodies. This clone, EMBL315, has a 14-kilobase (kb) insert that hybridizes to the previously isolated 3.9-kb fragment of the grsB gene, which encodes the 155-kilodalton ornithine-activating domain of gramicidin S synthetase 2. Deletion and subcloning experiments with the 14-kb insert located the grsA structural gene and its putative promoter on a 4.5-kb PvuII fragment which encoded the full-length 120-kilodalton protein in Escherichia coli. In addition, hybridization analysis revealed that the 5' end of the grsB gene is located approximately 3 kb from the grsA structural gene. Furthermore, these studies indicated that grsA and grsB are transcribed in opposite orientations.
Krause
M
M
Institut für Biochemie und Molekulare Biologie, Technische Universität Berlin, Federal Republic of Germany.
Marahiel
M A
MA
D000591:Amino Acid Isomerases / Q000235:genetics*; D001407:Bacillus / Q000235:genetics*; D015139:Blotting, Southern; D015153:Blotting, Western; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D006096:Gramicidin / Q000096:biosynthesis*; D009097:Multienzyme Complexes / Q000235:genetics*; D010453:Peptide Synthases / Q000235:genetics*; D015183:Restriction Mapping
D004269:DNA, Bacterial; D009097:Multienzyme Complexes; D006096:Gramicidin; D000591:Amino Acid Isomerases; D010453:Peptide Synthases; C020831:gramicidin-S-synthetase 2