[Improving the reproducibility of bone marrow culture by means of a salt-conditioned medium as a definite source of colony-stimulating activity].
Bone-marrow stem cell culture allows an insight into the regulatory system of normal and disturbed haemopoiesis. However, the prerequisite for it is that defined conditions of cultures are created. One step in this direction is the application of conditional media as a source of colony stimulating activity. The capacity of various conditioned media to stimulate proliferation and differentiation of human bone-marrow cells was examined and compared with the traditional feeder technique. In this respect the addition of 15% of umbilical cord conditioned medium proved to be well suited to achieve reproducible results.
Müller
A
A
Universitäts-Kinderklinik Jussuf Ibrahim Jena, Abteilung für Hämatologie, Onkologie und Immunologie, DDR.
John
A
A
John
U
U
Zintl
F
F
D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002455:Cell Division; D003115:Colony-Stimulating Factors / Q000032:analysis*; D003470:Culture Media; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans
D003115:Colony-Stimulating Factors; D003470:Culture Media
Versuche zur Verbesserung der Reproduzierbarkeit von Knochenmarkkulturen durch den Einsatz konditionierter Medien als definierte Quellen koloniestimulierender Aktivität.