Immunotoxin mediated killing of clonable T-lymphocytes infiltrating an irreversibly rejected human renal allograft.
We isolated and treated in vitro with a novel CD5-specific saporin immunotoxin, referred to as OKT1-SAP, the cells infiltrating an irreversibly rejected renal allograft from a patient who rejected while on cyclosporine plus steroids and then failed to respond to multiple courses of high-dose steroids, intravenous OKT3 antibody, and local irradiation to the graft. We report here that under experimental conditions achievable in vivo the immunotoxin OKT1-SAP was capable of eliminating in vitro more than 95% of clonable T-lymphocytes infiltrating the rejected allograft of this patient despite their resistance to previous aggressive immunosuppression. To our knowledge, this is the first report of an immunotoxin-mediated suppression of the clonogenic growth of rejected renal allograft infiltrating T-lymphocytes.
Siena
S
S
Division of Medical Oncology, Istituto Nazionale Tumori, Milano, Italy.
Bregni
M
M
Formosa
A
A
Brando
B
B
Lappi
D A
DA
Bonadonna
G
G
Gianni
A M
AM
D000328:Adult; D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000276:immunology; D018956:CD5 Antigens; D002469:Cell Separation; D002478:Cells, Cultured; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006084:Graft Rejection; D006801:Humans; D007165:Immunosuppression Therapy; D000922:Immunotoxins / Q000494:pharmacology*; D016030:Kidney Transplantation; D008297:Male; D009699:N-Glycosyl Hydrolases; D010641:Phenotype; D010940:Plant Proteins / Q000494:pharmacology*; D054789:Ribosome Inactivating Proteins, Type 1; D000076985:Saporins; D013601:T-Lymphocytes / Q000187:drug effects*
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D018956:CD5 Antigens; D000922:Immunotoxins; D010940:Plant Proteins; D054789:Ribosome Inactivating Proteins, Type 1; D009699:N-Glycosyl Hydrolases; D000076985:Saporins