MB-02 cells undergo fetal to adult globin gene switching in response to erythropoietin.
The recently described MB-02 human cell line requires Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) for continuous growth and terminally differentiates into enucleate, hemoglobinized cells in response to erythropoietin. Here, analysis of globin production now demonstrates that uninduced MB-02 cells produce alpha globin and the fetal globin chains G gamma and A gamma in a ratio of 1:3. Addition of erythropoietin results in de novo synthesis of beta globin chains and a marked increase in total Hb/cell. Thus, the MB-02 cell line partially recapitulates the fetal to adult globin switch that occurs during erythroid and human fetal development and provides a new clonal human erythroid progenitor system for investigating the biochemical and molecular events involved in globin gene switching.
Perrine
S P
SP
Department of Neoplastic Diseases, Hahnemann University, Philadelphia, PA.
Miller
B A
BA
Morgan
D A
DA
D002460:Cell Line; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D004921:Erythropoietin / Q000494:pharmacology*; D005319:Fetal Hemoglobin / Q000096:biosynthesis / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects*; D005796:Genes / Q000187:drug effects*; D005914:Globins / Q000096:biosynthesis / Q000235:genetics; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000494:pharmacology*; D006441:Hemoglobin A / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D004921:Erythropoietin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D005914:Globins; D006441:Hemoglobin A; D005319:Fetal Hemoglobin