Assessment of the sephadex technique for selection of X-bearing human sperm by analysis of sperm chromosomes, deoxyribonucleic acid and Y-bodies.
The effectiveness of sephadex sperm filtration on altering the sex ratio of human sperm was tested using three different methods. For each ejaculate the ratio of X- and Y-bearing sperm was analysed before and after sephadex filtration using three different methodologies: sperm chromosome analysis after fusion of human sperm with hamster oocytes, deoxyribonucleic acid analysis using the Y-preferential probe pS4 and the fluorescent Y-body test. In 182 sephadex-treated sperm analysed by sperm chromosomes the sex ratio (55.5%X) did not differ significantly from 226 untreated sperm (54.7%X) from the same semen samples. The DNA and Y-body tests demonstrated variability in both degree and pattern of enrichment for replicates but no consistent enrichment for X-chromosome-bearing sperm was observed for any one fraction after sephadex gel filtration.
Beckett
T A
TA
Genetics Clinic, Alberta Children's Hospital, Calgary, Canada.
Martin
R H
RH
Hoar
D I
DI
D001345:Autoradiography; D002869:Chromosome Aberrations; D025063:Chromosome Disorders; D002874:Chromosome Mapping; D004247:DNA / Q000032:analysis; D003911:Dextrans; D005821:Genetic Techniques; D006801:Humans; D007202:Indicators and Reagents; D007621:Karyotyping; D008297:Male; D012015:Reference Standards; D013094:Spermatozoa / Q000502:physiology*; D014960:X Chromosome; D014998:Y Chromosome
D003911:Dextrans; D007202:Indicators and Reagents; D004247:DNA; C025614:sephadex