[Establishment and characterization of phenotypically distinct cell lines from a single chromophilic renal cell carcinoma].
Two phenotypically different permanent cell lines (A and B) were established from a single human chromophilic renal cell carcinoma. The cells of cell line A were large and spindle-shaped, exhibiting a mean doubling time of 32 hours and a saturation density of 85,000 cells/sqcm. The cells of cell line B were smaller and polygonal, exhibiting a mean doubling time of 19 hours and a saturation density of 370,000 cells/sqcm. Cell line A proved to be non-tumorigenic after heterotransplantation into nude mice, whereas cell line B produced rapidly growing carcinomas. These transplant tumors were non-metastatic after subcutaneous implantation, but highly metastatic after renal implantation indicating that the biological behaviour of malignant tumors may be affected by the host tissue.
Gerharz
C D
CD
Gabbert
H E
HE
Störkel
S
S
Moll
R
R
Mellin
W
W
Ramp
U
U
Thoenes
W
W
D002292:Carcinoma, Renal Cell / Q000473:pathology*; D006801:Humans; D007680:Kidney Neoplasms / Q000473:pathology*; D008855:Microscopy, Electron, Scanning; D014407:Tumor Cells, Cultured / Q000473:pathology
Etablierung und Charakterisierung von phänotypisch unterschiedlichen Zellinien aus einem chromophilzelligen Nierenkarzinom.