Stereochemical course of catalysis by the Tetrahymena ribozyme.
The group I intron from Tetrahymena catalyzes phosphodiester transfer reactions on various RNA substrates. A modified RNA substrate with a phosphorothioate group in one stereoisomeric form at the site of reaction was synthesized in order to determine the stereochemical course of an RNA-catalyzed reaction. The reaction product was digested with a stereospecific nuclease to determine the configuration of the product phosphorothioate. The reaction occurs with inversion of configuration at phosphorus, implying an in-line pathway for the reaction.
Rajagopal
J
J
Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
Doudna
J A
JA
Szostak
J W
JW
D000818:Animals; D002384:Catalysis; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005091:Exons; D006151:Guanosine / Q000378:metabolism; D007438:Introns; D008968:Molecular Conformation; D009841:Oligonucleotides / Q000378:metabolism; D010758:Phosphorus; D012313:RNA / Q000138:chemical synthesis / Q000378:metabolism; D012322:RNA Precursors / Q000378:metabolism; D012326:RNA Splicing; D016337:RNA, Catalytic; D012335:RNA, Ribosomal / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism; D013329:Structure-Activity Relationship; D013604:T-Phages / Q000201:enzymology; D013698:Templates, Genetic; D013768:Tetrahymena / Q000235:genetics*; D013873:Thionucleotides / Q000378:metabolism
D009841:Oligonucleotides; D012322:RNA Precursors; D016337:RNA, Catalytic; D012335:RNA, Ribosomal; D013873:Thionucleotides; D006151:Guanosine; D010758:Phosphorus; D012313:RNA; D012321:DNA-Directed RNA Polymerases; D012260:Ribonucleases