Immunoglobulins in bile and serum of the rabbit associated with protection after Vibrio cholerae infection and vaccination.
Previous studies have shown that cholera, as well as protective immunity against infection with Vibrio cholerae, can be induced in the rabbit. This protection is long-lasting (up to 30 months) and is characterized on challenge by rapid, symptom-free disappearance of V. cholerae from the intestine; we therefore believe this to be vibriocidal protection. In this study, we analysed the humoral and secretory immune response against various subcellular V. cholerae components in vibriocidally protected, non-vibriocidally protected, and unprotected animals. Only vibriocidal protection was found to be associated with high levels of biliary IgA directed against lipopolysaccharide O antigen. We did not find such a correlation between either type of protection and response in serum. Therefore, anti-lipopolysaccharide antibodies are essential in protection against experimental infection with V. cholerae.
Rijpkema
S G
SG
National Institute of Public Health and Environmental Hygiene, Bilthoven, The Netherlands.
Jansen
W H
WH
Gielen
H
H
Guinée
P A
PA
D000372:Agglutination Tests; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001646:Bile / Q000276:immunology*; D002771:Cholera / Q000276:immunology* / Q000517:prevention & control; D002772:Cholera Toxin / Q000276:immunology; D022121:Cholera Vaccines / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D007071:Immunoglobulin A, Secretory / Q000096:biosynthesis; D007136:Immunoglobulins / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000276:immunology; D019081:O Antigens; D011817:Rabbits; D012463:Saliva / Q000276:immunology; D014611:Vaccination; D014734:Vibrio cholerae / Q000276:immunology*
D000942:Antigens, Bacterial; D022121:Cholera Vaccines; D007071:Immunoglobulin A, Secretory; D007136:Immunoglobulins; D008070:Lipopolysaccharides; D019081:O Antigens; D002772:Cholera Toxin