title: Albumin and alpha-fetoprotein gene expression in various nonhepatic rat tissues.
abstract: Albumin and alpha-fetoprotein (AFP), two major serum proteins, are synthesized predominantly in the liver and yolk sac of mammals. In the present paper we report on the developmental expression of the corresponding genes in nonhepatic rat tissues. Significant quantities of mature albumin and AFP mRNAs were revealed in kidney, pancreas, heart, and lung of fetal and/or newborn rats using dot blot and Northern blot assays. Very low levels of these mRNA sequences were also detected in adult kidney and pancreas using sensitive RNA-cDNA solution hybridization assays. In situ hybridization analysis revealed that the albumin and AFP gene transcripts are present in the tubular cells of the 20-day-old fetal kidney. In order to elucidate further the mechanisms governing this expression, we studied the chromatin structure and methylation pattern in the 5'-end of these two genes. A faint band, corresponding to a specific DNase I-hypersensitive site upstream from the albumin gene, was detected in the fetal and neonatal kidney nuclei but not in adult kidney. For both genes, a site CG, demethylation of which is correlated with expression in liver and hepatoma cell lines, is highly methylated in fetal kidney even though AFP and albumin genes are expressed. Taken together, these results show the presence of a cell population in the rat kidney that actively transcribes both the albumin and AFP genes. The expression of these genes may be mediated by mechanisms differing in at least some steps from those exerted in the liver.
authors: Laboratoire d'Enzymologie, Centre National de la Recherche Scientifique, Gif-sur- Yvette, France.
authors: J L
authors: JL
authors: Nahon
authors: I
authors: I
authors: Tratner
authors: A
authors: A
authors: Poliard
authors: F
authors: F
authors: Presse
authors: M
authors: M
authors: Poiret
authors: A
authors: A
authors: Gal
authors: J M
authors: JM
authors: Sala-Trepat
authors: L
authors: L
authors: Legrès
authors: G
authors: G
authors: Feldmann
authors: D
authors: D
authors: Bernuau
mesh_terms: D000818:Animals; D002460:Cell Line; D002843:Chromatin / Q000032:analysis; D004247:DNA / Q000378:metabolism; D003850:Deoxyribonuclease I / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D007668:Kidney / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000235:genetics; D008745:Methylation; D009693:Nucleic Acid Hybridization; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012709:Serum Albumin / Q000235:genetics*; D014158:Transcription, Genetic; D000509:alpha-Fetoproteins / Q000235:genetics*
chemical_list: D002843:Chromatin; D012333:RNA, Messenger; D012709:Serum Albumin; D000509:alpha-Fetoproteins; D004247:DNA; D003850:Deoxyribonuclease I