Isolation of a mycotoxin (gliotoxin) from a bovine udder infected with Aspergillus fumigatus.
A bovine udder infected with Aspergillus fumigatus was analysed by physico-chemical methods (thin layer chromatography, high performance liquid chromatography and direct exposure probe-mass spectrometry) for the presence of mycotoxins. Gliotoxin, a fungal metabolite with cytotoxic and immunosuppressive properties was isolated for the first time from naturally infected tissue. The gliotoxin concentration analysed (9.2 mg kg-1 udder) was approximately 100 times higher than the concentration known to produce morphological changes of cells. Gliotoxin may play an important role in the establishment and development of an infection with A fumigatus.
Bauer
J
J
Institute for Medical Microbiology, Infectious and Epidemic Diseases, Veterinary Faculty, University of Munich, F.R.G.
Gareis
M
M
Bott
A
A
Gedek
B
B
D000818:Animals; D001228:Aspergillosis / Q000382:microbiology / Q000662:veterinary*; D001232:Aspergillus fumigatus / Q000378:metabolism*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005260:Female; D005912:Gliotoxin / Q000096:biosynthesis / Q000302:isolation & purification*; D008321:Mammary Glands, Animal / Q000032:analysis* / Q000382:microbiology; D013058:Mass Spectrometry; D008414:Mastitis, Bovine / Q000382:microbiology*; D015394:Molecular Structure; D009183:Mycotoxins / Q000302:isolation & purification*
D009183:Mycotoxins; D005912:Gliotoxin