[A new approach to titrating active enzyme centers upon the use of surface-active micellar substances in organic solvents].
Micellar solutions of surfactant in organic solvents with rubber additions are proposed for determination of active enzyme concentration. A kinetic theory of enzymatic reactions in reversed micellar systems is developed, suggesting the intermicellar transport of the substrate to be the limiting step in viscous medium. Under these conditions, it is shown that fraction of the product formed after quick transformation of the substrate located in the enzyme-containing micelles depends upon active enzyme concentration and aggregation number of surfactant molecules. The proposed approach is used for the active-site titration of trypsin and cellobiase and for the determination of the aggregation number of Aerosol OT (AOT) molecules in the ternary system AOT/water/cyclohexane.
Pshezhetskiĭ
A V
AV
Kovalysheva
G V
GV
Chernoglazov
V M
VM
Levashov
A V
AV
Kliachko
N L
NL
D001665:Binding Sites; D003102:Colloids; D003510:Cyclohexanes; D004143:Dioctyl Sulfosuccinic Acid; D004563:Electrochemistry; D004798:Enzymes; D006868:Hydrolysis; D007700:Kinetics; D008823:Micelles; D012997:Solvents; D013501:Surface-Active Agents; D014357:Trypsin; D001617:beta-Glucosidase
D003102:Colloids; D003510:Cyclohexanes; D004798:Enzymes; D008823:Micelles; D012997:Solvents; D013501:Surface-Active Agents; D004143:Dioctyl Sulfosuccinic Acid; D001617:beta-Glucosidase; D014357:Trypsin
Novyĭ podkhod k titrovaniiu aktivnykh tsentrov fermentov pri ispol'zovanii mitsell poverkhnostno-aktivnykh veshchestv v organicheskikh rastvoriteliakh.