title: O-antigen from Bradyrhizobium japonicum lipopolysaccharide inhibits intercellular (symplast) communication between soybean (Glycine max) cells.
abstract: The technique of fluorescence redistribution after photobleaching was utilized to measure intercellular movement of low molecular weight fluorescent hydrophilic substances across the cell wall/membrane interface between contiguous soybean (Glycine max (L.) Merr. cv. Mandarin) root cells (SB-1 cell line) in tissue culture. Lipopolysaccharide (LPS) purified from Bradyrhizobium japonicum R110d, a Gram-negative bacterium that normally infects and induces nodulation in soybean roots in vivo, inhibits intercellular communication between the soybean cells in a dose-dependent manner. In contrast, LPS from noninfecting strains failed to yield the same effect. The inhibitory activity of the LPS was localized to the O-antigen region of the LPS.
authors: Department of Biochemistry, Michigan State University, East Lansing 48824.
authors: P K
authors: PK
authors: Gharyal
authors: S C
authors: SC
authors: Ho
authors: J L
authors: JL
authors: Wang
authors: M
authors: M
authors: Schindler
mesh_terms: D000942:Antigens, Bacterial / Q000302:isolation & purification*; D002450:Cell Communication; D002460:Cell Line; D004926:Escherichia coli / Q000276:immunology; D005452:Fluoresceins; D006090:Gram-Negative Bacteria / Q000276:immunology / Q000502:physiology*; D008070:Lipopolysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D008856:Microscopy, Fluorescence; D019081:O Antigens; D018521:Plant Physiological Phenomena; D013025:Glycine max
chemical_list: D000942:Antigens, Bacterial; D005452:Fluoresceins; D008070:Lipopolysaccharides; D019081:O Antigens; C018506:diacetylfluorescein