Synthesis of alpha 1-acid glycoprotein by the human prostate.
In order better to define the extent of protein synthesis capacity of the human prostate, we have studied the translation of selected serum proteins using isolated poly(A)+ RNA preparations and the rabbit reticulocyte lysate system. The translation of alpha 1-acid glycoprotein could be conclusively demonstrated but there was no apparent translation of albumin and plasmatic transferrin. Labeled alpha 1-acid glycoprotein was identified by specific immunoprecipitation with a commercial anti alpha 1-acid glycoprotein antiserum and correct processing by canine pancreatic microsomal membranes. Furthermore, we have shown by the immunoperoxidase technique that alpha 1-acid glycoprotein was indeed localized mainly in prostatic epithelial cells in 2 out of 2 patients with benign prostatic hypertrophy and in 3 out of 11 patients with prostatic adenocarcinoma. The significance of the synthesis and secretion of alpha 1-acid glycoprotein by prostatic cell themselves is presently unknown. However, we think that it could represent an interesting subject to explore further in relation with prostatic inflammation.
Dubé
J Y
JY
Laboratory of Hormonal Bioregulation, Laval University Hospital Centre, Ste-Foy, Québec, Canada.
Paradis
G
G
Têtu
B
B
Tremblay
R R
RR
D002474:Cell-Free System; D004848:Epithelium / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D007425:Intracellular Membranes / Q000378:metabolism; D008297:Male; D009961:Orosomucoid / Q000096:biosynthesis*; D011467:Prostate / Q000378:metabolism*; D011470:Prostatic Hyperplasia / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012709:Serum Albumin / Q000235:genetics; D014168:Transferrin / Q000235:genetics
D009961:Orosomucoid; D012333:RNA, Messenger; D012709:Serum Albumin; D014168:Transferrin