[Biological activity of human alpha-interferons studied using specific antisera].
Preparation of highly active rabbit antisera (AS) to human recombinant alpha 2-interferon and their use for studying biological properties of natural and plasmid alpha-interferons are described. By exhaustion of AS by alpha 3-interferon there were prepared practically monospecific AS not reacting with antigenic determinants of alpha 3-interferon. It was found that alpha 3-interferon represented a significant portion of human lymphoblastoid interferons and was included in PH-labile alpha-interferon from serum of patients with Kaposi carcinoma. AS to alpha 2-interferon completely neutralized antiviral and antiproliferative activity of the homologous subtype alpha-interferon and stimulation of cytotoxicity of human natural killer cells induced by it. It neutralized also the same effects of the heterologous subtypes (alpha 3 and alpha F/D) and leukocytic interferon, but the neutralization level was lower. The results of the study confirmed the polyfunctional nature of the interferon molecule.
Korsun
N S
NS
Nosik
D N
DN
Novokhatskiĭ
A S
AS
Trushinskaia
G N
GN
D000818:Animals; D000918:Antibody Specificity; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D007106:Immune Sera / Q000276:immunology* / Q000302:isolation & purification; D007114:Immunization / Q000379:methods; D007117:Immunization, Secondary; D007370:Interferon Type I / Q000032:analysis / Q000276:immunology* / Q000494:pharmacology; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000032:analysis / Q000276:immunology* / Q000494:pharmacology; D007372:Interferons / Q000032:analysis / Q000276:immunology* / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000187:drug effects; D009500:Neutralization Tests; D011817:Rabbits; D011994:Recombinant Proteins / Q000032:analysis / Q000276:immunology / Q000494:pharmacology; D013997:Time Factors
D007106:Immune Sera; D007370:Interferon Type I; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins; D007372:Interferons
Issledovanie biologicheskoĭ aktivnosti chelovecheskikh al'fa-interferonov pri pomoshchi spetsificheskikh antisyvorotok.