title: Sea-urchin RNAs displaying differences in developmental regulation and in complementarity to a collagen exon probe.
abstract: Sea-urchin embryo RNAs of 9 kb and 7 kb hybridise with a collagen-coding probe. The delta Tm of the hybrids indicates a 70% sequence identity between these RNA regions. Both RNAs are localised in the pluteus endomesoderm, but accumulate over different developmental periods: the 9 kb RNA first appears in the blastula and reaches a maximum concentration during the gastrula stages, while the 7 kb RNA is first detected in the gastrula and is at maximal concentration in the pluteus larva. Animalization by transient exposure of the early stage embryo to Zn2+ alters the developmental profile of the 9 kb collagen mRNA in a way that is clearly different from responses of other mRNAs whose accumulations are initiated during the blastula stage (Nemer, M. (1986) Dev. Biol. 114, 214-224).
authors: Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, PA 19111.
authors: M
authors: M
authors: Nemer
authors: P
authors: P
authors: Harlow
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D003094:Collagen / Q000235:genetics*; D015342:DNA Probes; D004625:Embryo, Nonmammalian / Q000502:physiology; D005091:Exons; D005306:Fertilization; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012313:RNA / Q000235:genetics*; D012617:Sea Urchins / Q000196:embryology / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D015342:DNA Probes; D012313:RNA; D003094:Collagen