Early effects of G- and GM-CSF upon HL-60 proliferation and differentiation.
Effects of human recombinant G- and GM-CSF upon HL-60 myeloid leukemic cell differentiation and proliferation have been studied. Minimal morphologically apparent differentiation was noted with treatment up to 7 days and concentrations up to 1000 units/ml. Cell surface marker analysis disclosed modest increases of MO1 and HLA-Dr expression following treatment with G-CSF/GM-CSF, for 2-4 days. Macromolecular synthesis rates following 24-hr exposures to CSF disclosed stimulation of [3H]uridine greater than [3H]thymidine greater than [3H]leucine by GM-CSF only. Proliferation was also assessed by flow cytometric DNA histogram analysis which also disclosed greater increases in the percentage of S + G2/M cells following GM-rather than G-CSF treatment. This study documents subtle early effects of G- and GM-CSF upon HL-60 proliferation and differentiation. Differentiative effects were relatively more marked with G-CSF while proliferative effects were more marked with GM-CSF.
Elias
L
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Department of Medicine, University of New Mexico School of Medicine, Albuquerque.
Van Epps
D E
DE
D014408:Biomarkers, Tumor / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D007938:Leukemia / Q000378:metabolism / Q000473:pathology; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology*
D014408:Biomarkers, Tumor; D003115:Colony-Stimulating Factors; D006133:Growth Substances; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor