A rapid staining technique for Leishmania parasites in splenic aspirate smears.
A quick and suitable method for staining Leishmania donovani and other blood protozoa is described. The method, which could have wide applications, is a modification of Field's staining technique as used for malaria parasites. It uses the same stains in a different order. The method has been tried on smears containing malaria and trypanosomes, and the results were good. It is very cheap and requires no special apparatus or training.
Chunge
C N
CN
Department of Medical Microbiology, College of Health Sciences, Nairobi, Kenya.
Ngige
S
S
Bwibo
C R
CR
Mulega
P C
PC
Kilonzo
J F
JF
Kibati
F
F
Owate
J
J
D000818:Animals; D006801:Humans; D007893:Leishmania donovani / Q000302:isolation & purification*; D007898:Leishmaniasis, Visceral / Q000175:diagnosis; D008288:Malaria / Q000175:diagnosis; D013154:Spleen / Q000469:parasitology*; D013194:Staining and Labeling; D014352:Trypanosomiasis / Q000175:diagnosis