Cross-reactive epitope with Klebsiella pneumoniae nitrogenase in articular tissue of HLA-B27+ patients with ankylosing spondylitis.
Synovial tissues from patients with ankylosing spondylitis or reactive arthritis were examined by an immunoperoxidase technique, using antisera to synthetic peptides representing antigens shared between HLA-B27.1 and Klebsiella pneumoniae nitrogenase. With either antiserum, all HLA-B27+ patients with synovial inflammation showed strong immunoperoxidase staining in synovial lining cells, vascular endothelium, and infiltrating inflammatory cells. These findings indicate that antigens showing cross-reactivity between HLA-B27.1 and Klebsiella nitrogenase epitopes are strongly expressed within inflamed synovial tissues of HLA-B27+ patients.
Husby
G
G
Department of Medicine, University of Florida School of Medicine, Gainesville.
Tsuchiya
N
N
Schwimmbeck
P L
PL
Keat
A
A
Pahle
J A
JA
Oldstone
M B
MB
Williams
R C
RC
D001168:Arthritis / Q000276:immunology; D002358:Cartilage, Articular / Q000276:immunology*; D003429:Cross Reactions; D000939:Epitopes; D015235:HLA-B Antigens / Q000032:analysis; D015796:HLA-B27 Antigen; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D007711:Klebsiella pneumoniae / Q000201:enzymology*; D009591:Nitrogenase / Q000276:immunology; D013167:Spondylitis, Ankylosing / Q000276:immunology*; D013582:Synovial Fluid / Q000276:immunology
D000939:Epitopes; D015235:HLA-B Antigens; D015796:HLA-B27 Antigen; D007371:Interferon-gamma; D009591:Nitrogenase