A human cytochrome P-450 is recognized by anti-liver/kidney microsome antibodies in autoimmune chronic hepatitis.
1- Anti-liver/kidney microsome autoantibodies type 1 (anti-LKM1), observed in some children with chronic active hepatitis, were used to isolate their antigen in human liver microsomes. A protein, called P-LKM1 was thus purified. This protein was recognized by a rabbit antiserum directed against the related human cytochromes P-450 bufI and P-450 bufII. 2- A human liver microsomal protein immunoprecipitated with anti-LKM1 sera was also recognized by anti cytochromes P-450 bufI/II antibodies. 3- Anti-LKM1 antibodies potently inhibited microsomal bufuralol 1'-hydroxylation. These results displayed the possible identity between cytochrome P-450 bufI/II and LKM1 antigen.
Kiffel
L
L
INSERM U 75, CHU Necker-Enfants Malades, Paris.
Loeper
J
J
Homberg
J C
JC
Leroux
J P
JP
D001323:Autoantibodies / Q000276:immunology*; D001324:Autoantigens / Q000032:analysis / Q000276:immunology; D001327:Autoimmune Diseases / Q000276:immunology*; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006521:Hepatitis, Chronic / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007163:Immunosorbent Techniques; D007527:Isoenzymes / Q000276:immunology; D007668:Kidney / Q000276:immunology* / Q000648:ultrastructure; D008861:Microsomes / Q000276:immunology; D008862:Microsomes, Liver / Q000276:immunology*
D001323:Autoantibodies; D001324:Autoantigens; D000939:Epitopes; D007527:Isoenzymes; D003577:Cytochrome P-450 Enzyme System