Neutralizing monoclonal antibody specific for alpha-bungarotoxin: preparation and characterization of the antibody, and localization of antigenic region of alpha-bungarotoxin.
We prepared an alpha-bungarotoxin-specific monoclonal antibody that neutralizes the biological activity of the toxin in vivo. The antigenic determinant combining specifically with this antibody was determined on the basis of cross-reaction experiments using three other long neurotoxins and peptide fragments of alpha-bungarotoxin. The antigenic determinant was located on the peptide fragment containing S34-S35-R36-G37-K38, which forms a part of the expected site that binds to the acetylcholine receptor proteins.
Kase
R
R
Department of Molecular Physiology, Tokyo Metropolitan Institute of Medical Science, Japan.
Kitagawa
H
H
Hayashi
K
K
Tanoue
K
K
Inagaki
F
F
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D002038:Bungarotoxins / Q000032:analysis / Q000276:immunology*; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis*; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000032:analysis / Q000276:immunology; D011487:Protein Conformation; D012697:Serine Endopeptidases
D000911:Antibodies, Monoclonal; D002038:Bungarotoxins; D000939:Epitopes; D010446:Peptide Fragments; D012697:Serine Endopeptidases; C036813:lysyl endopeptidase