Reconstitution of ionic channels from human heart.
This report is the first description of single ion channels from human myocardium. Using explanted human left ventricular tissue, we have studied three K-conducting and two anion-conducting channels. We report our observations of gating and ionic selectivity properties of these channels which, we argue, derive from both sarcolemmal and sarcoplasmic reticulum membranes. We postulate that one channel is the K-conducting channel (SR K channel) from human cardiac sarcoplasmic reticulum. Another channel is similar to a dimeric Cl- channel from Torpedo electroplax, displaying two equally spaced levels of open state conductance.
Hill
J A
JA
Department of Medicine, Duke University Medical Center, Durham, NC 27710.
Coronado
R
R
Strauss
H C
HC
D000818:Animals; D018118:Chloride Channels; D002712:Chlorides / Q000378:metabolism; D004285:Dogs; D004553:Electric Conductivity; D006801:Humans; D066298:In Vitro Techniques; D007473:Ion Channels / Q000378:metabolism*; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D015221:Potassium Channels / Q000378:metabolism; D012508:Sarcolemma / Q000378:metabolism; D012519:Sarcoplasmic Reticulum / Q000378:metabolism; D013045:Species Specificity; D014101:Torpedo / Q000378:metabolism
D018118:Chloride Channels; D002712:Chlorides; D007473:Ion Channels; D008565:Membrane Proteins; D015221:Potassium Channels