title: Biochemical changes in the liver of fish, Tilapia mossambica (Peters), during continuous exposure to monocrotophos.
abstract: The purpose of the present investigation was to determine the effect of continuous exposure to the organophosphate monocrotophos at 2.5 ppm for over a period of 2 through 45 days on protein, RNA, and DNA contents and on 5'-nucleotidase activity in the liver of Tilapia mossambica. Protein content was decreased by 45% after 5 days, returned to control levels at 10-30 days, and again decreased by 45 days. DNA content was decreased by 2 days, returned to control values by 5 days, and remained constant throughout the exposure. In contrast, RNA content was significantly lower starting from 2 through 45 days of exposure. 5'-Nucleotidase activity showed a transient increase at 5 and 30 days of monocrotophos exposure. These results indicate that monocrotophos altered the protein, DNA, and RNA contents and the 5'-nucleotidase activity levels as early as 2 and 5 days. However, these changes were reversed by 10 days and after a short period of recovery, the alterations reappeared. This supports our earlier histological observations of hepatic pathology during monocrotophos exposure.
authors: Department of Zoology, M.S. University of Baroda, India.
authors: U M
authors: UM
authors: Joshi
authors: A K
authors: AK
authors: Desai
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis; D005399:Fishes / Q000378:metabolism*; D007306:Insecticides / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008999:Monocrotophos / Q000633:toxicity*; D009699:N-Glycosyl Hydrolases / Q000032:analysis; D011506:Proteins / Q000032:analysis; D012313:RNA / Q000032:analysis
chemical_list: D007306:Insecticides; D011506:Proteins; D012313:RNA; D008999:Monocrotophos; D004247:DNA; D009699:N-Glycosyl Hydrolases; C030681:purine nucleosidase