Detection of multiple, novel reverse transcriptase coding sequences in human nucleic acids: relation to primate retroviruses.
A variety of chemically synthesized oligonucleotides designed on the basis of amino acid and/or nucleotide sequence data were used to detect a large number of novel reverse transcriptase coding sequences in human and mouse DNAs. Procedures involving Southern blotting, library screening, and the polymerase chain reaction were all used to detect such sequences; the polymerase chain reaction was the most rapid and productive approach. In the polymerase chain reaction, oligonucleotide mixtures based on consensus sequence homologies to reverse transcriptase coding sequences and unique oligonucleotides containing perfect homology to the coding sequences of human T-cell leukemia virus types I and II were both effective in amplifying reverse transcriptase-related DNA. It is shown that human DNA contains a wide spectrum of retrovirus-related reverse transcriptase coding sequences, including some that are clearly related to human T-cell leukemia virus types I and II, some that are related to the L-1 family of long interspersed nucleotide sequences, and others that are related to previously described human endogenous proviral DNAs. In addition, human T-cell leukemia virus type I-related sequences appear to be transcribed in both normal human T cells and in a cell line derived from a human teratocarcinoma.
Shih
A
A
Department of Biochemistry, New York University School of Medicine, New York 10016.
Misra
R
R
Rush
M G
MG
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000096:biosynthesis / Q000235:genetics*; D015342:DNA Probes; D004279:DNA, Viral / Q000096:biosynthesis / Q000235:genetics*; D005784:Gene Amplification; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D012313:RNA / Q000032:analysis; D012194:RNA-Directed DNA Polymerase / Q000235:genetics*; D012190:Retroviridae / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D004279:DNA, Viral; D012313:RNA; D004247:DNA; D012194:RNA-Directed DNA Polymerase