Platelet adhesion on to polyamide microcapsules coated with lipid bilayer membrane.
Polyamide microcapsules with diameters of 3-4 microns were coated with lipid bilayer membrane and their interaction with canine platelets was investigated. Platelet adhesion on to the microcapsules was significantly suppressed by the lipid-coating. Coating with dimyristoylphosphatidylcholine (liquid-crystalline state) reduced platelet adhesion on to the microcapsules to a greater extent than that with dipalmitoylphosphatidylcholine (gel state) at 37 degrees C. The surface properties of the microcapsule in adsorption of plasma proteins were also changed by lipid coating. The amount of gamma-globulin and fibrinogen adsorbed on to the microcapsule was slightly decreased by lipid coating, while the amount of adsorbed albumin was increased. Platelet adhesion on to the lipid-coated microcapsules was suppressed most strongly in the presence of gamma-globulin. Apparently platelet adhesion on to the polyamide microcapsules is controlled by the nature of lipid membrane and gamma-globulin adsorbed on to the microcapsules.
Kono
K
K
Department of Polymer Chemistry, Kyoto University, Japan.
Ito
Y
Y
Kimura
S
S
Imanishi
Y
Y
D000327:Adsorption; D000418:Albumins / Q000493:pharmacokinetics; D001672:Biocompatible Materials; D001792:Blood Platelets / Q000648:ultrastructure; D001798:Blood Proteins / Q000493:pharmacokinetics; D002214:Capsules; D004134:Dimyristoylphosphatidylcholine / Q000494:pharmacology; D005340:Fibrinogen / Q000494:pharmacology; D066298:In Vitro Techniques; D008567:Membranes, Artificial; D009757:Nylons; D010743:Phospholipids / Q000493:pharmacokinetics*; D010973:Platelet Adhesiveness / Q000187:drug effects; D013499:Surface Properties; D005719:gamma-Globulins / Q000493:pharmacokinetics
D000418:Albumins; D001672:Biocompatible Materials; D001798:Blood Proteins; D002214:Capsules; D008567:Membranes, Artificial; D009757:Nylons; D010743:Phospholipids; D005719:gamma-Globulins; D005340:Fibrinogen; D004134:Dimyristoylphosphatidylcholine