Antigenic sites in carcinoembryonic antigen.
The epitope reactivities of 52 well-characterized monoclonal antibodies (Mabs) against carcinoembryonic antigen from 11 different research groups were studied using competitive solid-phase immunoassays. About 60% of all possible combinations of Mabs as inhibitors and as the primary binding antibody were investigated. The inhibition data were analyzed by a specially developed computer program "EPITOPES" which measures concordance and discordance in inhibition patterns between Mabs. The analysis showed that 43 of the 52 Mabs (83%) could be classified into one of five essentially noninteracting epitope groups (GOLD 1-5) containing between four and 15 Mabs each. The epitopes recognized by the Mabs belonging to groups 1 to 5 were peptide in nature. With one or two possible exceptions non-classifiable Mabs were either directed against carbohydrate epitopes (4 Mabs) or were inactive in the tests used. Within epitope groups GOLD 1, 4, and 5 two partially overlapping subgroups were distinguished. Mabs with a high degree of carcinoembryonic antigen specificity generally belonged to epitope groups GOLD 1 and 3.
Hammarstrom
S
S
University of Umeå.
Shively
J E
JE
Paxton
R J
RJ
Beatty
B G
BG
Larsson
A
A
Ghosh
R
R
Bormer
O
O
Buchegger
F
F
Mach
J P
JP
Burtin
P
P
D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D007158:Immunologic Techniques; D012984:Software
D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D000939:Epitopes