title: Galanin inhibits insulin secretion by direct interference with exocytosis.
abstract: Electrically permeabilized RINm5F cells were used to study whether galanin inhibits insulin secretion distally to the generation of soluble second messengers. Ca2+-induced insulin secretion was inhibited by the neuropeptide in a dose-dependent manner. Galanin appears to act via a G-protein as pertussis toxin treatment abolished the effect. GTP (100 microM), GDP (100 microM) and a low dose of GTP gamma S (10 microM) did not affect galanin-mediated inhibition of secretion. In contrast, at 100 microM, GTP gamma S attenuated and GDP beta S abolished the effect of the peptide. We conclude that galanin inhibits exocytosis directly by a mechanism involving a G-protein.
authors: Division de Biochimie Clinique, Centre Médical Universitaire, Genève, Switzerland.
authors: S
authors: S
authors: Ullrich
authors: C B
authors: CB
authors: Wollheim
mesh_terms: D002118:Calcium / Q000494:pharmacology; D002460:Cell Line; D005089:Exocytosis / Q000187:drug effects*; D019204:GTP-Binding Proteins / Q000502:physiology; D019004:Galanin; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006153:Guanosine Diphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D010455:Peptides / Q000494:pharmacology*; D037342:Pertussis Toxin; D015290:Second Messenger Systems; D013873:Thionucleotides / Q000494:pharmacology; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
chemical_list: D007328:Insulin; D010455:Peptides; D013873:Thionucleotides; D010566:Virulence Factors, Bordetella; D006153:Guanosine Diphosphate; D016244:Guanosine 5'-O-(3-Thiotriphosphate); C023427:guanosine 5'-O-(2-thiodiphosphate); D006160:Guanosine Triphosphate; D019004:Galanin; D037342:Pertussis Toxin; D019204:GTP-Binding Proteins; D002118:Calcium