Immunochemical detection of multiple conformations within a 36 base pair oligonucleotide.
A 36 base pair chimeric oligonucleotide containing a central core of DNA duplex flanked by RNA/DNA hybrid at each end was synthesized. These distinct regions of the oligonucleotide adopt different conformations which were detected with antibody probes. Enzyme linked immunosorbent assays (ELISA) and a gel electrophoresis retardation assay were used to demonstrate the binding of antibodies which recognize B-DNA, Z-DNA and RNA/DNA hybrid. The DNA duplex core of this oligonucleotide adopts the B-conformation in 0.14 M NaCl. In high salt solution (4 M NaCl) the DNA core adopts the Z-conformation. The RNA/DNA hybrid at the ends of the oligomer adopt a conformation which is distinct from both B-DNA and A-RNA.
Sanford
D G
DG
Department of Biochemistry, Tufts University Health Sciences Campus, Boston, MA 02111.
Kotkow
K J
KJ
Stollar
B D
BD
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D001482:Base Composition; D004247:DNA; D004797:Enzyme-Linked Immunosorbent Assay; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D012313:RNA
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D012313:RNA; D004247:DNA