Further investigation on the high-conductance ion channel of the inner membrane of mitochondria.
By use of the patch-clamp technique, the inner membrane of mouse liver and heart mitochondria is shown to contain a highly conductive (around 100 pS in symmetrical 150 mM KCl) and voltage-dependent ion channel. This channel closely resembles that previously found in cuprizone-treated mouse liver inner mitochondrial membrane. The paper discusses the electrical properties of the channel and its possible physiological function. The reconstitution in giant liposomes of a partially purified ox heart inner membrane fraction containing the channel and the use of various inhibitors are also presented.
Sorgato
M C
MC
Dipartimento di Chimica Biologica, Universitá di Padova, Italy.
Moran
O
O
De Pinto
V
V
Keller
B U
BU
Stuehmer
W
W
D000818:Animals; D003471:Cuprizone / Q000494:pharmacology; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D004553:Electric Conductivity; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000378:metabolism; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D008081:Liposomes; D008564:Membrane Potentials; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D009840:Oligomycins / Q000494:pharmacology
D007473:Ion Channels; D008081:Liposomes; D009840:Oligomycins; D004024:Dicyclohexylcarbodiimide; D003471:Cuprizone