A one-step, low background coomassie staining procedure for polyacrylamide gels.
Present Coomassie staining procedures require hours of destaining and/or have high backgrounds. This one-step staining procedure is easier, gives lower background with no loss in sensitivity, uses less chemicals, requires less time, and can be followed by silver stain if increased sensitivity is desired after analyzing the results.
Zehr
B D
BD
Laboratory of Tumor Immunology, Guthrie Research Institute, Guthrie Clinic/Medical Center, Sayre, Pennsylvania 18840-1692.
Savin
T J
TJ
Hall
R E
RE
D000180:Acrylic Resins; D001671:Biochemistry / Q000379:methods*; D012394:Rosaniline Dyes; D012997:Solvents; D013194:Staining and Labeling / Q000379:methods
D000180:Acrylic Resins; D012394:Rosaniline Dyes; D012997:Solvents; C016680:polyacrylamide gels; C048139:Coomassie blue