The tropomyosin mRNAs of mouse striated muscles: molecular cloning of beta-tropomyosin.
The nucleotide sequence corresponding to the complete coding region and much of the 5' and 3' untranslated regions of the skeletal muscle-specific mouse beta-tropomyosin mRNA was determined from overlapping cDNA clones isolated from a library of recombinants in pBR322. When one of these was used as a probe to detect tropomyosin mRNAs expressed after fusion of mouse myoblasts in culture, species were detected in addition to those corresponding to known tropomyosins of striated muscle. One of these was also detected in the leg muscle of 12 day-old mice. The identities of these species are uncertain, but they may correspond to alternatively spliced products of the same RNA transcripts that give rise to the predominant tropomyosin isoforms of striated muscle.
McInnes
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Department of Biochemistry, University of Glasgow, U.K.
Leader
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DP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D015342:DNA Probes; D051379:Mice; D008969:Molecular Sequence Data; D009132:Muscles / Q000032:analysis; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000235:genetics; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D014335:Tropomyosin / Q000235:genetics*
D015342:DNA Probes; D012333:RNA, Messenger; D014335:Tropomyosin; D011061:Poly A; D012313:RNA; D004247:DNA