GABAA-receptor function in hippocampal cells is maintained by phosphorylation factors.
Gamma aminobutyric acid (GABA) mediates fast synaptic inhibition in the central nervous system by activating the chloride-permeable GABAA channel. The GABAA conductance progressively diminishes with time when the intracellular contents of hippocampal neurons are perfused with a minimal intracellular medium. This "run down" of the GABA-activated conductance can be prevented by the inclusion of magnesium adenosine triphosphate and calcium buffer in the intracellular medium. The amount of chloride conductance that can be activated by GABA is determined by competition between a calcium-dependent process that reduces the conductance and a phosphorylation process that maintains the conductance.
Stelzer
A
A
Department of Neurology, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
Kay
A R
AR
Wong
R K
RK
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000502:physiology; D002712:Chlorides / Q000502:physiology; D004533:Egtazic Acid / Q000494:pharmacology / Q000031:analogs & derivatives; D004553:Electric Conductivity; D006168:Guinea Pigs; D006624:Hippocampus / Q000502:physiology*; D066298:In Vitro Techniques; D007473:Ion Channels / Q000502:physiology; D008274:Magnesium / Q000494:pharmacology; D009433:Neural Inhibition; D010766:Phosphorylation; D011963:Receptors, GABA-A / Q000502:physiology*; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
D002712:Chlorides; D007473:Ion Channels; D011963:Receptors, GABA-A; D004533:Egtazic Acid; D005680:gamma-Aminobutyric Acid; D000255:Adenosine Triphosphate; D008274:Magnesium; C025603:1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid; D002118:Calcium