Amiloride increases the sensitivity of particulate guanylate cyclase to atrial natriuretic factor.
The natriuretic agent amiloride induces a shift of the dose-response curve of particulate guanylate cyclase to atrial natriuretic factor (ANF) to the left. The ANF concentration for half-maximal activation of guanylate cyclase is shifted from 20 to 3 nM in the presence of 100 microM amiloride. This effect is observed with GTP*Mn2+, but not with GTP*Mg2+ as substrate. Amiloride derivatives, which inhibit a specific Na+-channel, also shift the dose-response curve to the left. These data suggest that some of the effects of amiloride may be mediated by an increased sensitivity of particulate guanylate cyclase to ANF.
Heim
J M
JM
Labor für klinische Pharmakologie, Medizinische Klinik Innenstadt der Universität, München, FRG.
Ivanova
K
K
Gerzer
R
R
D000584:Amiloride / Q000031:analogs & derivatives / Q000494:pharmacology*; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000494:pharmacology*; D002417:Cattle; D006152:Cyclic GMP / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D006160:Guanosine Triphosphate / Q000378:metabolism; D006162:Guanylate Cyclase / Q000378:metabolism*; D007473:Ion Channels / Q000187:drug effects; D007700:Kinetics; D012964:Sodium / Q000378:metabolism
D007473:Ion Channels; C036852:magnesium GTP; C037047:manganese GTP; D000584:Amiloride; D009320:Atrial Natriuretic Factor; D006160:Guanosine Triphosphate; D012964:Sodium; D006162:Guanylate Cyclase; D006152:Cyclic GMP