[The effect of bleomycin on DNA synthesis in human cells: evidence for grouping of initiation of replicons in the S-period].
The effect of bleomycin (Blm) on DNA synthesis has been studied in a synchronous culture of human embryonic lung cells. The data obtained suggest that in the Blm presence in a medium (20 micrograms/ml) DNA synthesis initiation in new replicons is suppressed. The Blm action at different S-phase intervals has been shown to inhibit DNA synthesis unequally. Four discrete time intervals have been singled out in the course of the 10-hr S-phase in which a grouped initiation of replicon portions can be supposed. Together with the data on DNA replication in large-size replicon units (50-500 microns), the obtained results account well for the uneven DNA synthesis in S-phase, manifested by 3 or 4 peaks of [3H]-thymidine incorporation in pulse-labelled cells.
Liapunova
N A
NA
Lavrushina
O M
OM
Terekhov
S M
SM
D001761:Bleomycin / Q000494:pharmacology*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects*; D006801:Humans; D007399:Interphase / Q000187:drug effects*; D008168:Lung / Q000166:cytology / Q000196:embryology; D012093:Replicon / Q000187:drug effects*
D001761:Bleomycin; D004247:DNA
Vlianie bleomitsina na sintez DNK v kletkakh cheloveka: dokazatel'stvo sgruppirovannosti aktov initsiatsii replikonov v S-periode.