Mast cell lines produce lymphokines in response to cross-linkage of Fc epsilon RI or to calcium ionophores.
The cross-linkage of high affinity Fc epsilon receptors (Fc epsilon RI) on mast cells and basophils is central to the induction of allergic inflammatory responses. As a result of such cross-linkage, mast cells secrete a variety of preformed biologically active substances, such as histamine, and newly synthesized arachidonic acid metabolites. Here we show that cross-linkage of Fc epsilon RI on a series of nontransformed murine mast cell lines, or treatment of these cells with calcium ionophores, stimulates increased messenger RNA levels and secretion of a group of lymphokines classically produced by a subset of murine T cell lines (TH2 cells). These factors include interleukin-3 (a mast cell growth factor)s interleukin-4 (an IgE 'switch factor'), interleukin-5 (an eosinophil differentiation factor) and interleukin-6 (a factor controlling immunoglobulin secretion). The production of these polypeptide factors by mast cells may have great importance in the induction of allergic and anti-parasite inflammatory responses.
Plaut
M
M
Department of Medicine, Johns Hopkins University School of Medicine, Good Samaritan Hospital, Baltimore, Maryland 21239.
Pierce
J H
JH
Watson
C J
CJ
Hanley-Hyde
J
J
Nordan
R P
RP
Paul
W E
WE
D000818:Animals; D000941:Antigens / Q000276:immunology; D016718:Arachidonic Acid; D001095:Arachidonic Acids / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D003432:Cross-Linking Reagents; D003524:Cyclosporins / Q000494:pharmacology; D003600:Cytosol / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D004140:Dinitrophenols / Q000276:immunology / Q000494:pharmacology; D004987:Ethers / Q000494:pharmacology; D006636:Histamine Release; D007073:Immunoglobulin E / Q000276:immunology; D007377:Interleukin-3 / Q000096:biosynthesis; D015847:Interleukin-4; D015848:Interleukin-5; D015850:Interleukin-6; D007378:Interleukins / Q000096:biosynthesis; D015759:Ionomycin; D008222:Lymphokines / Q000096:biosynthesis* / Q000235:genetics; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D012333:RNA, Messenger / Q000096:biosynthesis; D011961:Receptors, Fc / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000276:immunology / Q000494:pharmacology
D000941:Antigens; D001095:Arachidonic Acids; D003432:Cross-Linking Reagents; D003524:Cyclosporins; D004140:Dinitrophenols; D004987:Ethers; D007377:Interleukin-3; D015848:Interleukin-5; D015850:Interleukin-6; D007378:Interleukins; D008222:Lymphokines; D012333:RNA, Messenger; D011961:Receptors, Fc; C034638:dinitrophenyl-bovine serum albumin; D015847:Interleukin-4; D012710:Serum Albumin, Bovine; D016718:Arachidonic Acid; D007073:Immunoglobulin E; D000001:Calcimycin; D015759:Ionomycin; D004247:DNA; D002118:Calcium