An in vivo analysis of c-myc and c-fos expression during terminal erythroid differentiation in mouse spleen progenitors.
Using thiamphenicol and scheduled bleeding, we were able to induce an adequate number of erythroid stem cells (CFU-e) in mice in order to conduct an in vivo study of the changing expression of c-myc and c-fos oncogenes during erythropoiesis. Results indicated that c-myc and c-fos are active in erythropoiesis and have a similar pattern of expression. A large decrease in expression of both c-myc and c-fos occurs when erythroid cells begin the biochemical transition into mature phenotypes, i.e., when RNA synthesis is down-regulated.
Badiavas
E V
EV
Department of Pharmacology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
Kaji
H
H
D000818:Animals; D002454:Cell Differentiation; D003114:Colony-Forming Units Assay; D005786:Gene Expression Regulation; D005914:Globins / Q000235:genetics; D006400:Hematocrit; D051379:Mice; D009693:Nucleic Acid Hybridization; D011519:Proto-Oncogenes; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D013154:Spleen / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology*; D013839:Thiamphenicol / Q000633:toxicity
D012313:RNA; D005914:Globins; D013839:Thiamphenicol