5-Aza-2'-deoxycytidine as inducer of differentiation and growth inhibition in mouse neuroblastoma cells.
We studied the effect of 5-aza-2'-deoxycytidine (5-AZA-CdR) on the differentiation of murine 41A3 neuroblastoma cells. Neuroblastoma cells treated with 0.1-1.0 microM 5-AZA-CdR underwent differentiation; markers of neuronal functions, such as acetylcholinesterase activity and growth of nerve fibers, were expressed at a higher level in the drug-treated cells than in the controls. This increased expression was accompanied by significant hypomethylation of newly synthesized DNA. A secondary event seemed to be a partial inhibition of DNA synthesis, cell proliferation and colony-forming activity. These effects were more pronounced than those caused by the related cytidine analog, 1-beta-D-arabinosil-cytosine (ARA-C). The results obtained suggest that 5-AZA-CdR may be an effective agent for the growth control of human neuroblastoma cells.
Bartolucci
S
S
Dipartimento di Chimica Organica e Biologica, Università di Napoli, Italy.
Estenoz
M
M
Longo
A
A
Santoro
B
B
Momparler
R L
RL
Rossi
M
M
Augusti-Tocco
G
G
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001374:Azacitidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000378:metabolism; D000077209:Decitabine; D008745:Methylation; D051379:Mice; D009447:Neuroblastoma; D014407:Tumor Cells, Cultured / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism
D000077209:Decitabine; D004247:DNA; D000110:Acetylcholinesterase; D001374:Azacitidine