Protein lysine 6-oxidase (lysyl oxidase) cofactor: methoxatin (PQQ) or pyridoxal?
1. Treatment of chick embryos with two lathyrogens lowered lysyl oxidase and increased collagen extractability. 2. Subsequent treatment with pyridoxal restored both parameters towards normal, whereas PQQ treatment was less effective. 3. These results suggest the requirement of a pyridoxal derivative for the formation of the enzyme, acting either as cofactor or because its formation requires some pyridoxal-dependent enzyme. The cochromatography of the enzyme with [3H]pyridoxine-derived radioactivity supports the cofactor role. 4. The conclusions of other authors that lysyl oxidase contains PQQ relates to enzymes from other species or to amine oxidases not characterised as lysyl oxidase.
Levene
C I
CI
University of Cambridge, Department of Pathology, England.
O'Shea
M P
MP
Carrington
M J
MJ
D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D000818:Animals; D001846:Bone Development / Q000187:drug effects; D002642:Chick Embryo; D003067:Coenzymes / Q000494:pharmacology*; D003094:Collagen / Q000302:isolation & purification; D006909:Hydroxyproline / Q000032:analysis; D066298:In Vitro Techniques; D008099:Liver / Q000032:analysis; D008112:Liver Glycogen / Q000032:analysis; D045542:PQQ Cofactor; D008249:Protein-Lysine 6-Oxidase / Q000378:metabolism*; D011730:Pyridoxal / Q000494:pharmacology*; D011732:Pyridoxal Phosphate / Q000032:analysis; D011736:Pyridoxine / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000494:pharmacology; D015363:Quinolones / Q000494:pharmacology*; D012663:Semicarbazides / Q000494:pharmacology; D013194:Staining and Labeling
D003067:Coenzymes; D008112:Liver Glycogen; D015363:Quinolones; D012663:Semicarbazides; C010059:carbamylhydrazine; D011730:Pyridoxal; D011732:Pyridoxal Phosphate; C004675:4-deoxypyridoxine; D045542:PQQ Cofactor; D003094:Collagen; D000594:Amino Acid Oxidoreductases; D008249:Protein-Lysine 6-Oxidase; D011736:Pyridoxine; D006909:Hydroxyproline