title: Spike--nucleocapsid interaction in Semliki Forest virus reconstructed using network antibodies.
abstract: Using in vitro immunization, we have reconstructed three consecutive steps of an idiotype network to show that the nucleocapsid of Semliki Forest virus contains a specific 'receptor' for the cytoplasmic tail of the E2 spike glycoprotein. This interaction could be the basis for the highly selective inclusion of viral glycoproteins--and exclusion of host cell surface proteins--during virus budding.
authors: Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510.
authors: D J
authors: DJ
authors: Vaux
authors: A
authors: A
authors: Helenius
authors: I
authors: I
authors: Mellman
mesh_terms: D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D002213:Capsid / Q000378:metabolism*; D000939:Epitopes; D007130:Immunoglobulin Idiotypes / Q000096:biosynthesis; D012672:Semliki forest virus / Q000254:growth & development* / Q000648:ultrastructure; D014764:Viral Proteins / Q000276:immunology / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D014764:Viral Proteins