Myelin breakdown in the posterior funiculus of the kitten after dorsal rhizotomy. A qualitative and quantitative light and electron microscopic study.
Morphological aspects of myelin breakdown in the posterior funiculus during Wallerian degeneration were studied in kittens subjected to lumbosacral dorsal rhizotomies 6-8 days after birth. The first sign of myelin breakdown was characterized by swollen or shrunken nerve fibers. Shortly thereafter there was an increased occurrence of collapsed myelin sheaths and later of rounded myelin bodies. Myelin was clearly seen in microglial cells. Correlative observations on Marchi-stained material indicted the simultaneous and frequent appearance of Marchi-positive bodies (MPB:s) and myelin bodies. Due to the rapidity of the degeneration process in the kitten, the increase in the occurrence of Marchi-positive granules (MPG:s) seemed to start concomitantly with increased occurrence of MPB:s. However, the frequent occurrence of MPG:s outlasted that for MPB:s. The findings indicate that the MPB:s may be the counterpart to myelin bodies and the MPG:s to lipid droplets. Microglial cells may be responsible for the primary uptake of degenerating myelin and the subsequent transformation of myelin bodies to lipid droplets. The much faster breakdown of myelin and elimination of lipid material in the degenerating posterior funiculus of the kitten, as compared to the adult, seemed to be due not only to the lower myelin content in the kitten, but also to a higher density of microglial and a greater efficiency in the myelin breakdown process in the degenerating posterior funiculus of the kitten.
Franson
P
P
Department of Anatomy, Karolinska Institutet, Stockholm, Sweden.
Ronnevi
L O
LO
D000818:Animals; D002415:Cats; D002818:Cordotomy; D008854:Microscopy, Electron; D009186:Myelin Sheath / Q000378:metabolism* / Q000648:ultrastructure; D009410:Nerve Degeneration; D013116:Spinal Cord / Q000503:physiopathology* / Q000648:ultrastructure; D013194:Staining and Labeling; D013997:Time Factors; D014855:Wallerian Degeneration