Culture of an epithelial cell line (MDCK) with a serum substitute.
This paper reports the suitability of culturing a line of dog kidney epithelial cells, MDCK, in the presence of a serum substitute, Ultroser G. Serial subcultivation with this product was possible for at least 10 passages without any change in cell shape and size, saturation density, dome-forming ability, transepithelial resistance, and growth curve. Adhesion of newly plated cells to plastic was somewhat lower than in fetal calf serum but the trypsin-harvesting kinetics were essentially the same. However, the membrane ion transport system was altered: cell sodium influx was greatly diminished, suggesting a deep change in the amiloride-sensitive Na+ channels; sodium efflux was highly enhanced (both active and passive).
Gutiérrez
M C
MC
Dept. of Health Sciences, Universidad Autónoma Metropolitana-I, Mexico City, DF Mexico.
Díaz-Pontones
D M
DM
López Vancell
M R
MR
Beaty
G
G
D000818:Animals; D001802:Blood Substitutes / Q000494:pharmacology*; D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003470:Culture Media; D004285:Dogs; D004553:Electric Conductivity; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology; D007473:Ion Channels / Q000378:metabolism; D007668:Kidney / Q000166:cytology* / Q000502:physiology; D009930:Organic Chemicals; D012964:Sodium / Q000378:metabolism
D001802:Blood Substitutes; D003470:Culture Media; D007473:Ion Channels; D009930:Organic Chemicals; C044609:Ultroser G; D012964:Sodium