Metachromatic procedure for fiber typing in chicken skeletal muscle.
Metachromatic staining of anterior latissimus dorsi, posterior latissimus dorsi, and sartorius muscles of domestic chicken with azure A and toluidine blue produced differentiation of muscle fiber types. The procedure is rapid, with optimum results obtained after 2 to 5 min incubation and 5 to 10 s of staining. Reaction of serial sections for myosin Ca++-adenosine triphosphatase with the usual procedure showed that the same fibers were typed similarly with both methods.
Sosnicki
A
A
Muscle Biology Laboratory, University of Wisconsin, Madison 53706.
Cassens
R G
RG
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002645:Chickens / Q000033:anatomy & histology*; D005260:Female; D006651:Histocytochemistry; D009132:Muscles / Q000032:analysis / Q000033:anatomy & histology* / Q000201:enzymology; D013194:Staining and Labeling
D000252:Calcium-Transporting ATPases