The alloantibody response in the allogeneically pregnant rat. VI. Detection in maternal serum of specific antigen-antibody complexes.
We have examined prepartum and postpartum sera from allogeneically pregnant rats for the presence of paternal antigen using a mAb sandwich assay. Paternal class I antigen, RT1Aa, was frequently detected in postpartum and prepartum rat sera. Postpartum sera from high responders also include anti-RT1Aa alloantibodies, some of which were complexed with the RT1Aa antigen. Although the concentration of antigen is low, the high uterine blood flow causes the amount of class I antigen entering the pregnant female to be in the low microgram per day range. Such an amount could have immunoregulatory significance.
Amsden
A F
AF
Department of Reproductive Biology, Case Western Reserve University, Cleveland, OH 44106.
Smith
R N
RN
Chirakalwasan
N
N
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex / Q000032:analysis*; D000939:Epitopes / Q000276:immunology; D005260:Female; D006649:Histocompatibility Antigens / Q000032:analysis / Q000276:immunology*; D007130:Immunoglobulin Idiotypes / Q000276:immunology; D007518:Isoantibodies / Q000096:biosynthesis*; D007700:Kinetics; D008297:Male; D049590:Postpartum Period; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000276:immunology*; D051381:Rats
D000888:Antibodies, Anti-Idiotypic; D000936:Antigen-Antibody Complex; D000939:Epitopes; D006649:Histocompatibility Antigens; D007130:Immunoglobulin Idiotypes; D007518:Isoantibodies; C038353:histocompatibility antigens RT, rat