An activity stain for proteins containing noncovalently bound flavin adenine dinucleotide.
A procedure has been developed to allow the visualization of FAD-containing proteins on polyacrylamide gels. The technique is based on the reconstitution of apo-D-amino acid oxidase with FAD and is thus specific for this cofactor. The stain is sensitive enough to detect 5 pmol of FAD and is therefore useful for the detection of flavoproteins in systems as complex as crude tissue or bacterial extracts.
Kitzler
J W
JW
Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710.
Fridovich
I
I
D001059:Apoproteins; D003605:D-Amino-Acid Oxidase; D004591:Electrophoresis, Polyacrylamide Gel; D005182:Flavin-Adenine Dinucleotide / Q000032:analysis*; D006735:Horseradish Peroxidase; D006861:Hydrogen Peroxide; D011506:Proteins / Q000032:analysis*; D013194:Staining and Labeling / Q000379:methods
D001059:Apoproteins; D011506:Proteins; D005182:Flavin-Adenine Dinucleotide; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D003605:D-Amino-Acid Oxidase