title: Differential stability of human interleukin 1 beta fragments expressed in yeast.
abstract: We have made deletions in a human interleukin 1 beta cDNA cloned in a yeast vector capable of directing the expression and secretion of foreign protein. Deletions at either the 5' or 3' end of the cDNA result in a dramatic drop in yield of protein. An investigation of some possible causes points to instability of the truncated peptides as a major factor contributing to low yield. The results suggest that correct folding of mature interleukin 1 into a protease resistant form requires almost the entire polypeptide.
authors: Sclavo Research Center, Siena, Italy.
authors: C
authors: C
authors: Baldari
authors: A
authors: A
authors: Massone
authors: G
authors: G
authors: Macchia
authors: J L
authors: JL
authors: Telford
mesh_terms: D001483:Base Sequence; D002872:Chromosome Deletion; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D007375:Interleukin-1 / Q000235:genetics*; D009154:Mutation; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000032:analysis / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000235:genetics
chemical_list: D007375:Interleukin-1; D012313:RNA