The pathomorphological diagnosis of acute leukaemias: cytology, cytochemistry and immunocytology.
In 74 cases of acute leukaemia and of the blastic phase of chronic granulocytic leukaemia (CML), monoclonal antibodies of the "VI" series and a few commercially available antibodies proved to be of valuable help in establishing a definite diagnosis. In 4 out of 35 cases of AML (FAB-M 1-5), and in 2 out of 16 of the blastic phase of CML only the use of monoclonal antibodies secured the diagnosis. In the group of acute lymphoid leukaemias subtypes corresponding to various levels of differentiation, were defined. The blasts of 3 patients out of the 74 did not express any of the markers studied. Two additional cases were investigated for platelet peroxidase and studied with the antiplatelet antibodies VIPL 1, 2 and 3 by the electron microscope. These cases proved to be acute megakaryoblastic leukaemias (acute myelofibrosis).
Kelényi
G
G
Department of Pathology, University Medical School, Pécs, Hungary.
Kalász
V
V
Matolcsy
A
A
D000208:Acute Disease; D000911:Antibodies, Monoclonal; D001792:Blood Platelets / Q000201:enzymology; D002469:Cell Separation; D006651:Histocytochemistry; D006801:Humans; D007947:Leukemia, Megakaryoblastic, Acute / Q000473:pathology*; D007951:Leukemia, Myeloid / Q000473:pathology*; D008854:Microscopy, Electron; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000473:pathology*
D000911:Antibodies, Monoclonal