Strong delayed-type hypersensitivity induced against house dust mite antigens in the mice.
The aim of this study was to assess the antigenicity of house dust mite antigens using delayed-type hypersensitivity (DTH) responses in mice. The crude extracts of Dermatophagoides pteronyssinus and Dermatophagoides farinae induced strong DTH responses and was transferred with T cells. Assessment of DTH responses to the house dust revealed that substantial cross-reactivity at T-cell level was observed between these two mite extracts. Almost all the fractions of the extract of D. pteronyssinus, which has a molecular weight of between 10 and 100 kilodaltons, elicited strong DTH responses. It was found that antigenicity of the mite extract was reduced by chemical denaturation with tannic acid.
Nakano
Y
Y
Division of Industrial Health, Osaka Prefectural Institute of Public Health, Japan.
Yoshida
M
M
Shibata
T
T
D000485:Allergens / Q000276:immunology*; D000818:Animals; D039741:Antigens, Dermatophagoides; D002850:Chromatography, Gel; D003520:Cyclophosphamide / Q000494:pharmacology; D000939:Epitopes; D005260:Female; D047348:Hydrolyzable Tannins; D006968:Hypersensitivity, Delayed / Q000139:chemically induced / Q000276:immunology*; D007116:Immunization, Passive; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D008925:Mites / Q000276:immunology*
D000485:Allergens; D039741:Antigens, Dermatophagoides; D000939:Epitopes; D047348:Hydrolyzable Tannins; D003520:Cyclophosphamide