Comparison of HMB-45 monoclonal antibody and S-100 protein in the immunohistochemical diagnosis of melanoma.
The value of HMB-45 mouse monoclonal antibody in the immunohistochemical diagnosis of melanoma was compared with that of two antibodies to S-100 protein. Tissue from 32 (91.4%) of the 35 melanomas studied reacted with HMB-45, however, none of the 98 nonmelanoma tumors stained with this antibody. In contrast, 31 (88.5%) melanomas and 24 (24.4%) of the nonmelanoma neoplasms expressed S-100 protein. These results indicated that HMB-45 monoclonal antibody has a higher specificity for melanoma cells than did S-100 protein and that, because of its reactivity in routinely processed tissues, it may be helpful in surgical pathology.
Ordóñez
N G
NG
Department of Pathology, U.T.M.D. Anderson Hospital, Houston, Texas 77030.
Ji
X L
XL
Hickey
R C
RC
D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D000951:Antigens, Neoplasm; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007202:Indicators and Reagents; D008545:Melanoma / Q000032:analysis / Q000473:pathology*; D058950:Melanoma-Specific Antigens; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000032:analysis; D009418:S100 Proteins / Q000032:analysis* / Q000276:immunology; D013194:Staining and Labeling / Q000379:methods
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D007202:Indicators and Reagents; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins; D009418:S100 Proteins