title: Organophosphate exposure inhibits non-specific esterase staining in human blood monocytes.
abstract: The objective of this study was to investigate the association between exposure to triaryl phosphate and monocyte nonspecific esterase staining activity in workers from a plant in Nitro, West Virginia. A cross-sectional study design was used to identify the exposed plant employees, and both a plant and a general population group. In addition to completing a questionnaire for each participant, 30 cc of blood were drawn and analyzed using four techniques, two morphological and two enzymatic. These procedures included a Technicon D-90 autoanalyzer, a Technicon H-6000 autoanalyzer, a manual enzymatic using alpha naphthyl butyrate, and a manual morphologic using Wright-Giesma stain. The results showed a decrease in nonspecific esterase staining with the Technicon D-90. No significant effect on monocyte numbers or esterase positivity was found with the other three techniques.
authors: Division of Environmental and Occupational Health, School of Public Health, University of Minnesota, Minneapolis 55455.
authors: J S
authors: JS
authors: Mandel
authors: N T
authors: NT
authors: Berlinger
authors: N
authors: N
authors: Kay
authors: J
authors: J
authors: Connett
authors: M
authors: M
authors: Reape
mesh_terms: D000328:Adult; D000368:Aged; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000037:antagonists & inhibitors*; D003430:Cross-Sectional Studies; D004784:Environmental Monitoring; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000201:enzymology*; D009784:Occupational Diseases / Q000097:blood* / Q000139:chemically induced; D009943:Organophosphorus Compounds / Q000009:adverse effects*; D011897:Random Allocation; D013194:Staining and Labeling
chemical_list: D009943:Organophosphorus Compounds; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase