title: A simple metabolic system for selection of hybrid hybridomas (tetradomas) producing bispecific monoclonal antibodies.
abstract: A metabolic selective system has been proposed for the selection of hybrid hybridomas (tetradomas) based on the introduction in one of the parental cell lines of two traits simultaneously--a recessive one (resistance to 8-azaguanine) and a dominant one (multidrug resistance). Tetradomas were selected in the presence of two selective agents: aminopterin and actinomycin D. Using this approach we produced tetradomas secreting bispecific MAbs to horseradish peroxidase and human alpha-fetoprotein.
authors: Laboratory of Immunochemistry, Cancer Research Center, USSR Academy of Medical Sciences, Moscow.
authors: A V
authors: AV
authors: Chervonsky
authors: A I
authors: AI
authors: Faerman
authors: L V
authors: LV
authors: Evdonina
authors: A K
authors: AK
authors: Jazova
authors: A R
authors: AR
authors: Kazarov
authors: A I
authors: AI
authors: Gussev
mesh_terms: D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D015139:Blotting, Southern; D002460:Cell Line; D004351:Drug Resistance / Q000235:genetics; D006735:Horseradish Peroxidase / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D000509:alpha-Fetoproteins / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000509:alpha-Fetoproteins; D006735:Horseradish Peroxidase