3'-end labeling of DNA fragments by AMV-reverse transcriptase.
The efficiency on 3'-end labeling of DNA fragments was compared between Klenow fragment of DNA polymerase I from E. coli and AMV-reverse transcriptase. In the case of Klenow fragment, the exonuclease activity of this enzyme rapidly removed the labeled dNMP incorporated in 3'-end of DNA fragment by the polymerase activity of it. AMV-reverse transcriptase caused no decrease of the labeled dNMP incorporated by the polymerase activity because it has no exonuclease activity. Therefore, AMV-reverse transcriptase is easier to use than Klenow fragment for labeling the 3'-end of DNA fragment.
Oyama
F
F
Mitsubishi-Kasei Institute of Life Sciences, Tokyo, Japan.
Kikuchi
R
R
Omori
A
A
Uchida
T
T
D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D004247:DNA; D004256:DNA Polymerase I; D004926:Escherichia coli / Q000201:enzymology; D007553:Isotope Labeling / Q000379:methods; D012194:RNA-Directed DNA Polymerase
D004247:DNA; D012194:RNA-Directed DNA Polymerase; D004256:DNA Polymerase I