Expression of human CD4 by two human-mouse interlineage hybrids.
Two hybrid cell lines expressing human CD4 were prepared by fusing human B-lymphoid cells with the mouse T-lymphoma BW5147. Hybrid TF42 was derived from a human B-lymphoblastoid line and TF53.1 from a human B-ALL. Variants of these hybrids expressing or lacking CD4 were isolated by sorting cells stained with the monoclonal antibody (mAb) OKT4 on a fluorescence-activated cell sorter (FACS). Cytogenetic, isoenzyme and DNA analysis confirmed the presence of human chromosome 12 in the CD4+ hybrids, and revealed that CD4 expression by TF42 was associated with multiple copies of this chromosome. Of seventy mAb recognizing human T-cell antigens screened on the CD4+ and CD4- variants of the two hybrids, only mAb recognizing CD4 and Leu 8 reacted with the CD4+ cells. These hybrids should be useful in the preparation, screening and analysis of anti-CD4 monoclonal antibodies, and in studies of CD4 epitopes recognized by HIV.
Taylor
G M
GM
Department of Medical Genetics, St Mary's Hospital, Manchester, UK.
Morten
J E
JE
Morten
H
H
Dodge
A B
AB
Ridway
J C
JC
Jones
P M
PM
Harris
R
R
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D002459:Cell Fusion; D004247:DNA / Q000032:analysis; D000939:Epitopes / Q000032:analysis; D004854:Herpesvirus 4, Human; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology*; D015448:Leukemia, B-Cell; D008223:Lymphoma; D051379:Mice; D008815:Mice, Inbred Strains; D010641:Phenotype; D011817:Rabbits; D013601:T-Lymphocytes; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000939:Epitopes; D004247:DNA