A cytotoxic monoclonal antibody that binds to human B cells, T cells, monocytes, and a subset of lymphomas.
Monoclonal antibodies have been derived against a variety of antigens on human leukocytes. We describe a complement-fixing, IgG2a, murine monoclonal antibody that was derived against ARH-77, a human plasma cell line. In a Western blot of a lysate of ARH-77, anti-ARH-77 recognized a 155 kD protein. On normal B cells, the antibody co-capped with surface immunoglobulin. Analysis of blood and lymph nodes indicated that the determinant recognized by the antibody is expressed by most B cells, T cells, monocytes, and lymphomas with surface immunoglobulin. The determinant was not present on granulocytes, platelets, mantle zone lymphocytes, or serum immunoglobulin. We conclude that anti-ARH-77 is a cytotoxic monoclonal antibody that recognizes a determinant shared by human mononuclear leukocytes. The determinant may be an exposed part of a membrane anchor or bridging protein associated with receptors on mononuclear cells.
Samoszuk
M K
MK
Pathology Department, University of California, Irvine 92717.
Gidanian
F
F
Lo-Hsueh
M
M
Rietveld
C
C
D000818:Animals; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007152:Immunologic Capping; D007963:Leukocytes, Mononuclear / Q000145:classification / Q000276:immunology*; D008223:Lymphoma / Q000145:classification / Q000276:immunology*; D051379:Mice; D009000:Monocytes / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes