In vivo 13C NMR spectroscopy of the human prostate.
The metabolism of the normal and hyperplastic human and rat prostate differs from that of other tissues in that this organ supports and secretes high concentrations of citrate. During the dedifferentiation process that is characteristic of neoplasia, the ability of the prostate to maintain these high citrate levels is markedly diminished. We undertook a natural abundance 13C NMR study of the human and rat prostate in vitro and the human organ in vivo in order to determine whether the amounts of citrate in the nonmalignant prostate were sufficiently high to be detected. We report here our success at detecting citrate via 13C NMR in all three of these systems.
Sillerud
L O
LO
Division of Life Sciences, Los Alamos National Laboratory, University of California, New Mexico 87545.
Halliday
K R
KR
Griffey
R H
RH
Fenoglio-Preiser
C
C
Sheppard
S
S
D000328:Adult; D000818:Animals; D002951:Citrates / Q000378:metabolism; D003937:Diagnosis, Differential; D006801:Humans; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D011467:Prostate / Q000378:metabolism / Q000473:pathology*; D011470:Prostatic Hyperplasia / Q000473:pathology; D011471:Prostatic Neoplasms / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains
D002951:Citrates