Effect of pancreastatin on pancreatic endocrine and exocrine secretion.
Pancreastatin is a novel peptide that was recently purified from extracts of the porcine pancreas. The present study shows that pancreastatin (10(-9)-10(-8) M) can stimulate release of insulin from both the isolated perfused rat pancreas and from cultured rat islet cells in the presence of a low, non-insulinotropic concentration of glucose (4.2 mM). Pancreastatin (10(-9) M) can also inhibit release of insulin stimulated by a high concentration of glucose (16.7 mM). Pancreastatin, at 10(-8) M, can enhance glucose (8.3 mM) induced release of insulin in the static islet cell incubation. In addition, pancreastatin (10(-9)-10(-8) M) can inhibit, in a dose-dependent fashion, cholecystokinin (CCK)-8 stimulated release of amylase from dispersed guinea pig pancreatic acini. Pancreastatin alone, however, did not affect basal release of amylase. Our study shows that pancreastatin can exert a direct effect on both pancreatic endocrine and exocrine secretion.
Ishizuka
J
J
Third Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
Asada
I
I
Poston
G J
GJ
Lluis
F
F
Tatemoto
K
K
Greeley
G H
GH
Thompson
J C
JC
D000681:Amylases / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D053379:Chromogranin A; D005947:Glucose / Q000494:pharmacology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology*; D010187:Pancreatic Hormones / Q000494:pharmacology*; D010189:Pancreatic Juice / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D011916:Rats, Inbred F344; D012016:Reference Values; D012844:Sincalide / Q000494:pharmacology
D053379:Chromogranin A; D007328:Insulin; D010187:Pancreatic Hormones; C050836:pancreastatin; D000681:Amylases; D005947:Glucose; D012844:Sincalide