Comparison between two peptide epitopes presented to cytotoxic T lymphocytes by HLA-A2. Evidence for discrete locations within HLA-A2.
An influenza B virus nucleoprotein (BNP) peptide, residues 82-94, defined by limited sequence homology with an HLA-A2-restricted peptide from influenza A matrix protein, was recognized by HLA-A2-restricted CTL. Reciprocal inhibition of T cell recognition by the two peptides suggest that the BNP peptide may have lower avidity for HLA-A2 molecules than the matrix peptide. The interaction between this peptide and HLA-A2 was explored by studying the CTL recognition of BNP 82-94 presented by mutant HLA-A2 molecules. Mutations at residues 9, 99, 70, 74, 152 and 156 were found to abolish T cell recognition of the BNP peptide. These results were compared with results previously obtained with the influenza A matrix peptide and suggest that the two peptides bind differently in the peptide binding site.
Robbins
P A
PA
Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, England.
Lettice
L A
LA
Rota
P
P
Santos-Aguado
J
J
Rothbard
J
J
McMichael
A J
AJ
Strominger
J L
JL
D000595:Amino Acid Sequence; D003429:Cross Reactions; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D015789:HLA-A2 Antigen / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D006801:Humans; D009981:Influenza B virus / Q000276:immunology; D009154:Mutation; D009698:Nucleoproteins / Q000276:immunology; D010455:Peptides / Q000235:genetics / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014763:Viral Matrix Proteins / Q000276:immunology
D000939:Epitopes; D015789:HLA-A2 Antigen; D009698:Nucleoproteins; D010455:Peptides; D014763:Viral Matrix Proteins