[Comparison of protein concentrations in saliva and serum].
Saliva and serum protein profiles composed of 12 proteins: IgA, IgG, IgM, albumin, transferrin, alpha 1-glycoprotein acid, alpha 2-macroglobulin, alpha 1-proteinase inhibitor, haptoglobine, C3C, C4 and CRP were obtained by laser nephelometry of aliquots of samples of non-stimulated whole saliva and blood taken at the same time. The population of 138 controls of both sexes was divided into 4 age groups: 1 (20 to 29 years), 2 (30 to 39 years), 3 (40 to 49 years), 4 (50 to 60 years). Using the techniques developed for saliva assay, 10 proteins were measurable: IgA, IgG, IgM, albumin, transferrin, alpha 1-glycoprotein acid, alpha 2-macroglobulin, alpha 1-proteinase inhibitor, haptoglobulin and C3C. The C4 fraction and CRP were present at concentrations lower than the detection limit i.e. at 1.8 and 2.05 mg/l. In the serum 11 proteins were analysed, CRP occurring at less than 0.01 g/l. The statistical two-factor variance analysis test (sex and age) demonstrated the statistically significant influence of age in the saliva for alpha 1-PI and alpha 2-M in the serum for Tf, alpha 2-M and alpha 1-PI, and sex in the serum for IgG, IgM, alpha 1-PI, alpha 2-M and also the interaction of both factors in the serum for alpha 2-M.
Grimoud
A M
AM
Département de Sciences Biologiques, Faculté de Chirurgie Dentaire, Université Paul-Sabatier, Toulouse.
Pontet
F
F
Rousselet
F
F
Lodter
J P
JP
Duffaut
D
D
Seguela
J P
JP
D000328:Adult; D000367:Age Factors; D000418:Albumins / Q000032:analysis; D000704:Analysis of Variance; D001798:Blood Proteins / Q000032:analysis*; D002097:C-Reactive Protein / Q000032:analysis; D003176:Complement C3 / Q000032:analysis; D015932:Complement C3c; D003181:Complement C4 / Q000032:analysis; D005260:Female; D006242:Haptoglobins / Q000032:analysis; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008297:Male; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry; D011506:Proteins / Q000032:analysis*; D012463:Saliva / Q000032:analysis* / Q000276:immunology; D012709:Serum Albumin / Q000032:analysis; D012737:Sex Factors; D014168:Transferrin / Q000032:analysis; D000515:alpha 1-Antitrypsin; D000511:alpha-Macroglobulins / Q000032:analysis
D000418:Albumins; D001798:Blood Proteins; D003176:Complement C3; D003181:Complement C4; D006242:Haptoglobins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011506:Proteins; D012709:Serum Albumin; D014168:Transferrin; D000515:alpha 1-Antitrypsin; D000511:alpha-Macroglobulins; D015932:Complement C3c; D002097:C-Reactive Protein
Comparaison des concentrations protéiques de la salive et du sérum.