Gold labeling with wheat germ agglutinin and RNAse on osmicated tissue embedded in epoxy resin.
Tissue of an insect, Lucilia cuprina, fixed conventionally in buffered glutaraldehyde and osmium and embedded in epoxy resin (epon or epon/araldite), provided sections which could readily be labeled with RNAse/gold and wheat germ agglutinin (WGA)/gold. This method offers labeling of tissues with improved contrast and allows the retrospective application of RNAse and WGA labeling to conventionally prepared tissues, without recourse to oxidizing/etching agents.
Brooks
E M
EM
CSIRO, Division of Entomology, Canberra, Australia.
Binnington
K C
KC
D000818:Animals; D002686:Chitin / Q000032:analysis*; D003102:Colloids; D004175:Diptera / Q000032:analysis; D004853:Epoxy Resins; D006046:Gold; D006651:Histocytochemistry / Q000379:methods*; D009993:Osmium Tetroxide; D012313:RNA / Q000032:analysis*; D012260:Ribonucleases; D014909:Wheat Germ Agglutinins
D003102:Colloids; D004853:Epoxy Resins; D014909:Wheat Germ Agglutinins; D002686:Chitin; D012313:RNA; D006046:Gold; D012260:Ribonucleases; D009993:Osmium Tetroxide