Immuno-crossreactivity between botulinum neurotoxin type C1 or D and exoenzyme C3.
Botulinum neurotoxin type D and exoenzyme C3 have been separately purified from Clostridium botulinum strain D-1873 to apparent homogeneity. Both ADP-ribosylated a rat liver cytosolic protein of 24 kDa. The N-terminal amino acid sequence of C3 was determined and showed a low degree of homology with those of the light and heavy chains of neurotoxins of various types which have been reported previously. However, a polyclonal antibody raised against C3 cross-reacted with the light chains, but not with the heavy chains, of type C1 and D neurotoxins. Furthermore, a monoclonal antibody recognizing the light chains of type C1 and D neurotoxins interacted with C3. These results suggest that the light chain of type C1 or D neurotoxin and exoenzyme C3 share at least one epitope in common with each other.
Toratani
S
S
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Japan.
Yokosawa
N
N
Yokosawa
H
H
Ishii
S
S
Oguma
K
K
D036002:ADP Ribose Transferases / Q000276:immunology*; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D001905:Botulinum Toxins / Q000276:immunology* / Q000302:isolation & purification; D003014:Clostridium botulinum / Q000201:enzymology / Q000378:metabolism; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D008969:Molecular Sequence Data
D000911:Antibodies, Monoclonal; D000939:Epitopes; D000246:Adenosine Diphosphate Ribose; D036002:ADP Ribose Transferases; D001905:Botulinum Toxins