IGF I induces differentiation in a transformed human keratinocyte line.
A comparison of normal epithelial cells with their transformed counterparts could lead to the definition of parameters related to growth and differentiation which are altered by viral transformation and which may be relevant to malignant changes in vivo. Using the SV40-transformed human keratinocyte line, SVK14, which exhibits characteristics of simple, nonkeratinizing epithelia, we have shown that IGF I stimulation of these cells results in extensive multilayering, increased cell size, accumulation of involucrin, modulation of keratin 18 and expression of keratins 14 and 10, whilst T-antigen expression is maintained in the multilayered cells. Since T-antigen expression is correlated directly with impairment of stratification and differentiation, it is interesting that treatment of SVK14 with a single growth factor. IGF I, results in molecular events characteristic of differentiating normal keratinocytes.
Kamalati
T
T
Department of Anatomy and Human Biology, Kings College London, Strand, UK.
Howard
M
M
Brooks
R F
RF
D000957:Antigens, Viral, Tumor / Q000096:biosynthesis; D002454:Cell Differentiation; D002455:Cell Division; D002461:Cell Line, Transformed; D005434:Flow Cytometry; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D015151:Immunoblotting; D007328:Insulin / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D015603:Keratinocytes / Q000166:cytology* / Q000187:drug effects; D007633:Keratins / Q000096:biosynthesis; D011498:Protein Precursors / Q000096:biosynthesis; D013539:Simian virus 40; D013002:Somatomedins / Q000494:pharmacology*
D000957:Antigens, Viral, Tumor; D006133:Growth Substances; D007328:Insulin; D011498:Protein Precursors; D013002:Somatomedins; C032253:involucrin; D007334:Insulin-Like Growth Factor I; D007633:Keratins