Purification of liver plasma membranes and localization of a protein galactosyltransferase.
Plasma membranes were isolated from mouse liver. The purification was achieved by a modified method of Ray (1970). The validity of this fractionation procedure is controlled by measurements of specific enzymatic activities and by electron microscopy. The purified plasma membranes were found to contain galactosyltransferase which transferred galactose from UDP-galactose onto endogenous acceptors. This activity requires Mn2+ for its catalytic activity.
Fernandez-Briera
A
A
Department of Biochemistry and Molecular Biology, University of Leon, Spain.
Louisot
P
P
Morelis
R
R
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000201:enzymology*; D002499:Centrifugation, Density Gradient; D005700:Galactosyltransferases / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000201:enzymology* / Q000648:ultrastructure; D008274:Magnesium / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron
D005700:Galactosyltransferases; D008274:Magnesium; D002118:Calcium