Trichinella spiralis: recognition of muscle larva antigens during experimental infection of swine and its potential use in diagnosis.
Longitudinal studies with Trichinella spiralis experimentally infected pigs were carried out to identify muscle larva antigens recognized during infection. This was approached using Western blot analysis and ELISA assays. Immunoblots of sera from experimentally infected pigs using total parasite extracts revealed five principal parasite antigens throughout infection. A similar pattern of antigen recognition was given by sera from backyard pigs in areas of Mexico, some of them endemic for Trichinella. Four of the five antigens recognized (MW 47, 52, 67, and 72 kDa) corresponded to surface/stichosomal antigens purified by monoclonal antibody NIM-M1. In addition, Western blots of excretions-secretions of muscle larva contained three (MW 52, 67, and 72 kDa) of the four surface/stichosomal components recognized by NIM-M1. Affinity-purified surface/stichosomal components, total soluble extracts, and excretory-secretory antigens of muscle larva were then evaluated in ELISA for detection of T. spiralis infections in experimentally infected, noninfected control, and 295 backyard pigs. These assays showed that purified surface/stichosomal components and excretory-secretory antigens increased the specificity of ELISA. These results suggest that muscle larva components purified by monoclonal antibody NIM-M1 are the major antigens recognized during infection of pigs with T. spiralis and therefore potentially useful for diagnosis of swine trichinellosis.
Arriaga
C
C
Department of Immunology, Instituto Nacional de Investigaciones Forestales y Agropecuarias, D.F. Mexico.
Muñiz
E
E
Morilla
A
A
Ortega-Pierres
G
G
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis* / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology; D015153:Blotting, Western / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D000939:Epitopes; D007814:Larva / Q000276:immunology; D009132:Muscles / Q000469:parasitology; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology; D014234:Trichinella / Q000276:immunology*; D014235:Trichinellosis / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*
D000947:Antigens, Helminth; D000954:Antigens, Surface; D000939:Epitopes