Production and control of specificity of a goat anti-bovine thymocytes antiserum.
The identification and the numeration of the lymphocytes in cattle is realized essentially by revealing the membranary properties of these cells: receptors for some lectins (i.e. peanut agglutinin or phytohemagglutinin), receptors for sheep red blood cells (SRBC), surface-specific antigens concerning T-lymphocytes and surface immunoglobulins concerning B-lymphocytes. The technique of the production in the goat of a specific anti-bovine T-lymphocyte antiserum (GABTA) is described. The GABTA shows a specific cytotoxic activity on 55% of the peripheral blood lymphocytes and more than 95% of the thymocytes. It inhibits, at 90%, the formation of the rosetting with SRBC. This reagent allows identification and a common and quick numeration of the T-lymphocytes in cattle.
Person
J M
JM
Institut d'Immunologie Animale et Comparée, Ecole Nationale Vétérinaire d'Alfort, Maisons Alfort, France.
Barrat
F
F
Pilet
C
C
D000818:Animals; D000917:Antibody Formation; D000961:Antilymphocyte Serum / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D002417:Cattle / Q000097:blood / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005260:Female; D006041:Goats; D007958:Leukocyte Count / Q000662:veterinary; D008213:Lymphocyte Activation; D011237:Predictive Value of Tests; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology
D000961:Antilymphocyte Serum; D000939:Epitopes