[Synthesis of poly (Lys-Ser-Glu) and study of its antigenic and immunogenic properties].
We have synthesized oligo- and polypeptides containing lysin, serine and glutamic acid residues. Di- and tripeptides were prepared by the successive elongation of C-terminus by means of the N,N'-dicyclohexylcarbodiimide or mixed anhydride methods. The oligopeptides were obtained by block condensation of pentafluorophenyl esters of tripeptides, and polypeptides by polycondensation of dipeptide 2,4,5-trichlorophenyl esters. The three amino acids (Lys, Ser, Glu) were shown to take part in formation of antigenic determinants, serine being probably the immunodominant amino acid, and dipeptide Ser-Glu- the most important component of the epitope Lys-Ser-Glu. Polypeptides (Lys-Ser-Glu)n induced cross-reacting or heteroclitic antibodies. The expression of immune response depended on genotype of experimental animals.
Khalikov
Sh Kh
ShKh
Valiev
R V
RV
Sharetskiĭ
A N
AN
Aristovskaia
L V
LV
D000818:Animals; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D000939:Epitopes / Q000276:immunology*; D007114:Immunization; D051379:Mice; D008808:Mice, Inbred CBA; D008970:Molecular Weight; D010455:Peptides / Q000138:chemical synthesis* / Q000276:immunology; D011487:Protein Conformation; D011817:Rabbits
D000939:Epitopes; D010455:Peptides; C057165:poly(lysyl-seryl-glutamic acid)
Sintez poly (Lys-Ser-Glu) i izuchenie ego antigennykh i immunogennykh svoĭstv.