[The surface glycolipid antigen specific for the internal cell mass of the mouse blastocyst and of the stem cells of murine teratocarcinoma F9].
A new monoclonal antibody that recognizes a new antigen on the surface of mouse teratocarcinoma F9 stem cells has been described. This antigen is a glycolipid as demonstrated by inhibition of immunofluorescence by different monosaccharides, glycoproteins and glycolipid fraction of F9 cells as well as by chemical analysis. Immunofluorescent staining of in vitro cultivated preimplantation mouse embryos has demonstrated that this antigen is specific only of internal cell mass cells of late blastocyst.
Anfimova
M L
ML
Bannikov
G A
GA
Troianovskiĭ
S M
SM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000302:isolation & purification; D000941:Antigens / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis; D001755:Blastocyst / Q000276:immunology*; D002478:Cells, Cultured; D054278:Embryonal Carcinoma Stem Cells; D000939:Epitopes / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006017:Glycolipids / Q000032:analysis / Q000276:immunology*; D007074:Immunoglobulin G / Q000032:analysis; D008563:Membrane Lipids / Q000032:analysis / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D014411:Neoplastic Stem Cells / Q000276:immunology*; D013724:Teratoma / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000941:Antigens; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D000939:Epitopes; D006017:Glycolipids; D007074:Immunoglobulin G; D008563:Membrane Lipids
Poverkhnostnyĭ glikolipidnyĭ antigen, spetsifichnyĭ dlia vnutrenneĭ kletochnoĭ massy blastotsisty mysheĭ i stvolovykh kletok myshinoĭ teratokartsinomy F9.