title: Diffusion properties of clathrin on the surface of isolated mouse liver nuclei by the fluorescence recovery after photobleaching technique.
abstract: Clathrin labeled with eosin maleimide showed physicochemical properties similar to the native clathrin. The diffusion coefficients of clathrin protomers and cages measured at 20 degrees C by the fluorescence recovery after photobleaching technique (FRAP) were found equal to (9 +/- 1).10(-8) cm2.s-1 and (3 +/- 0.4.10(-8) cm2.s-1, respectively. After incubation with isolated mouse liver nuclei suspended in an aqueous buffer, FRAP measurements showed that 78% of clathrin was immobilized on the nuclear surface. This immobile fraction might correspond to aggregates of molecules resembling coated pits. The mobile fraction had a diffusion coefficient of 2.5.10(-9) cm2.s-1 which was reduced seven times when the suspension medium of the nuclei contained 50% sucrose, showing that the aqueous phase exerted an important drag on the clathrin molecules motion diffusing on the nuclear surface.
authors: Centre de Biophysique Moléculaire, C.N.R.S., Orleans, France.
authors: P
authors: P
authors: Nandi
authors: P
authors: P
authors: Wahl
mesh_terms: D000818:Animals; D002417:Cattle; D002467:Cell Nucleus; D002499:Centrifugation, Density Gradient; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002966:Clathrin; D003034:Coated Pits, Cell-Membrane / Q000032:analysis; D004058:Diffusion; D004591:Electrophoresis, Polyacrylamide Gel; D004801:Eosine Yellowish-(YS) / Q000031:analogs & derivatives; D008099:Liver / Q000648:ultrastructure*; D051379:Mice; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D010777:Photochemistry; D014783:Viscosity
chemical_list: D002966:Clathrin; C033531:eosin maleimide; D004801:Eosine Yellowish-(YS)