The role of membrane bound sialic acid of rat mast cells in histamine release induced by compound 48/80 and derivatives as well as calcium.
Histamine release induced by compound 48/80 from rat mast cells is not dependent on extracellular Ca2+. Preincubation of mast cells with trypsin has only little effects on histamine release induced by this polycation. This work also demonstrates that histamine release induced by compound 48/80 and its analogues in the absence of extracellular Ca2+ depends on membrane bound sialic acid of the mast cell. Neuraminidase treatment of the cells in the presence of extracellular Ca2+ leads to histamine liberation. These findings suggest that sialic acid residues of the mast cell membrane constitute the site at which polycations exert their stimulatory actions of histamine liberation.
Adamczyk-Engelmann
P
P
University Ulm, FRG.
Bader
H
H
D000818:Animals; D001665:Binding Sites; D002118:Calcium / Q000494:pharmacology; D002412:Cations; D002462:Cell Membrane / Q000378:metabolism*; D006636:Histamine Release / Q000187:drug effects*; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D019158:N-Acetylneuraminic Acid; D009439:Neuraminidase / Q000494:pharmacology; D011073:Polyamines; D000071228:Polyelectrolytes; D011108:Polymers / Q000494:pharmacology; D051381:Rats; D012794:Sialic Acids / Q000378:metabolism*; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology*
D002118:Calcium; D002412:Cations; D019158:N-Acetylneuraminic Acid; D009439:Neuraminidase; D011073:Polyamines; D000071228:Polyelectrolytes; D011108:Polymers; D012794:Sialic Acids; D003189:p-Methoxy-N-methylphenethylamine