Reactive oxygen species induce antigenic changes in DNA.
Reactive oxygen species (ROS) are released at sites of inflammation during the respiratory burst which accompanies the phagocytic process. Using an in vitro system to simulate this process we have shown that ROS induce antigenic changes in DNA. More specifically, results of experiments using ROS scavengers have shown that hydroxyl radicals produced in close proximity to DNA-bound metal ions play a predominant role. ROS-mediated attack resulted in increased binding of anti-DNA antibodies to the denatured DNA. These changes were detected using IgG, IgA and IgM isotype binding to antibodies in systemic lupus erythematosus sera. Of these the IgA isotype was most discriminating in its detection of hydroxyl radical-induced damage.
Blount
S
S
Department of Biochemistry/Rheumatology, Selly Oak Hospital, Birmingham, England.
Griffiths
H R
HR
Lunec
J
J
D000818:Animals; D000906:Antibodies / Q000276:immunology; D001205:Ascorbic Acid / Q000494:pharmacology; D002417:Cattle; D004247:DNA / Q000187:drug effects / Q000276:immunology*; D003676:Deferoxamine / Q000494:pharmacology; D000939:Epitopes / Q000276:immunology*; D005609:Free Radicals; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006878:Hydroxides / Q000494:pharmacology; D017665:Hydroxyl Radical; D007106:Immune Sera / Q000276:immunology; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D009691:Nucleic Acid Denaturation / Q000187:drug effects; D010100:Oxygen / Q000494:pharmacology*; D013890:Thiourea / Q000494:pharmacology
D000906:Antibodies; D000939:Epitopes; D005609:Free Radicals; D006878:Hydroxides; D007106:Immune Sera; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M; D017665:Hydroxyl Radical; D004247:DNA; D006861:Hydrogen Peroxide; D013890:Thiourea; D003676:Deferoxamine; D001205:Ascorbic Acid; D010100:Oxygen