Comparison of histochemical and biochemical methods in the evaluation of estrogen and progesterone receptor level in human breast cancer. A study of 118 cases.
Unselected surgical specimens from 118 cancerous breasts of female patients were studied by histochemistry for the presence of cellular estrogen (ER) and progesterone (PR) receptors. The receptor tracers used were 17 beta-estradiol-6-carboxymethyl-oxim-bovine serum albumin - fluorescein isothiocyanate and 11 alpha-hydroxyprogesterone-hemisuccinate-bovine serum albumin-tetramethylrodamine isothiocyanate conjugate (Fluoro-CepTM). Out of 118 carcinomas 77 (67%) proved to be positive in the ER and 79 (66%) in the PR test. The histochemical results were compared with the results of the biochemical assay in 70 cases. A positive correlation was found in 57 cases.
Mohácsy
J
J
2nd Central Electron Microscope Laboratory, Semmelweis Medical University, Budapest, Hungary.
Kádár
A
A
Vallent
K
K
Duffek
L
L
Vass
K
K
D001943:Breast Neoplasms / Q000032:analysis*; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000032:analysis*; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D011960:Receptors, Estrogen / Q000032:analysis*; D011980:Receptors, Progesterone / Q000032:analysis*; D013194:Staining and Labeling
D011960:Receptors, Estrogen; D011980:Receptors, Progesterone