[Electron microscopic-radioautographic study of cultured human epidermocytes].
A split thickness skins of burned patients were treated by trypsin and received the epidermocyte suspension. In that suspensions the content of basal keratinocytes was 10 to 70% in dependence from the treating conditions. As a rule, cultivation was a success only in those cases, when among basal keratinocytes of initial suspension were cells, synthesized DNA. Cultures were grown more rapidly under decrease of ion calcium concentration in nutritional medium up to 0.14 mM and in the presence of cell-free surface of substratum. At such conditions connections between cells were weakened, desmosomes disappeared, stratification and colony formation were broken.
Pal'tsyn
A A
AA
Tumanov
V P
VP
Basagina
L S
LS
Serov
G G
GG
Badikova
A K
AK
D000328:Adult; D001345:Autoradiography; D002455:Cell Division; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004817:Epidermis / Q000378:metabolism / Q000648:ultrastructure*; D006651:Histocytochemistry; D006801:Humans; D007633:Keratins / Q000378:metabolism; D008854:Microscopy, Electron; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D014316:Tritium; D007633:Keratins; D004247:DNA; D013936:Thymidine
Elektronno-radioavtograficheskoe issledovanie kul'tiviruemykh épidermotsitov cheloveka.