An in vitro model of allergen-dependent IgE synthesis by human B lymphocytes: comparison of the response of an atopic and a non-atopic individual to Dermatophagoides spp. (house dust mite).
An allergen-dependent in vitro model of immunoglobulin E (IgE) synthesis by human B cells is reported. Using this model, it is demonstrated that polyclonal T cells and CD4+ Dermatophagoides spp. (house dust mite)-specific T-cell clones derived from an atopic, house dust mite (HDM)-allergic individual are able to support IgE synthesis by autologous B cells. The helper activity was interleukin-4 (IL-4) dependent as only cloned T cells expressing detectable mRNA for IL-4 were able to induce IgE synthesis without the addition of exogenous IL-4. Peripheral and cloned T cells reactive with HDM could also be identified from a non-atopic individual but neither population was able to support IgE production even in the presence of exogenous IL-4.
O'Hehir
R E
RE
Department of Allergy and Clinical Immunology, National Heart and Lung Institute, Brompton Hospital, Middlesex.
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Quint
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Moqbel
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Kay
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Zanders
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Lamb
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D000485:Allergens / Q000276:immunology*; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002478:Cells, Cultured; D004391:Dust; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis; D015847:Interleukin-4; D007378:Interleukins / Q000276:immunology; D008925:Mites / Q000276:immunology*; D012221:Rhinitis, Allergic, Perennial / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D000485:Allergens; D004391:Dust; D000939:Epitopes; D007074:Immunoglobulin G; D007378:Interleukins; D015847:Interleukin-4; D007073:Immunoglobulin E