Cryopreservation of first-stage and infective third-stage larvae of Strongyloides stercoralis.
Infective third-stage larvae of Strongyloides stercoralis were frozen over liquid nitrogen and remained infective to dogs when thawed. Successful cryopreservation depended on a 30-60-min incubation in a cryoprotectant (10% DMSO and 10% dextran) before freezing and thawing the frozen larvae into RPMI. First-stage larvae could also be frozen by this method. Thawed first-stage larvae remained viable and continued their development to third-stage larvae, which were shown to be infective to dogs.
Nolan
T J
TJ
University of Pennsylvania School of Veterinary Medicine, Department of Pathobiology, Philadelphia 19104-6050.
Aikens
L M
LM
Schad
G A
GA
D000818:Animals; D003451:Cryoprotective Agents / Q000494:pharmacology; D003911:Dextrans / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004285:Dogs / Q000469:parasitology; D005615:Freezing; D007814:Larva / Q000187:drug effects; D011309:Preservation, Biological; D013321:Strongyloides / Q000187:drug effects / Q000472:pathogenicity
D003451:Cryoprotective Agents; D003911:Dextrans; D004121:Dimethyl Sulfoxide