Telomere terminal transferase activity from Euplotes crassus adds large numbers of TTTTGGGG repeats onto telomeric primers.
A telomere terminal transferase activity was identified in developing macronuclear extracts from Euplotes crassus. The activity was essentially unregulated in vitro: up to 50 tandem repeats of the Euplotes telomeric repeat sequence TTTTGGGG were added onto synthetic telomeric oligonucleotide primers. Both the structure of the telomere substrate and its 3'-terminal sequence were recognized. The activity was destroyed by low concentrations of RNase A.
Shippen-Lentz
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Department of Molecular Biology, University of California, Berkeley 94720.
Blackburn
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EH
D000818:Animals; D002875:Chromosomes / Q000378:metabolism*; D016798:Ciliophora / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D004247:DNA / Q000378:metabolism; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism*; D003720:Densitometry; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D012259:Ribonuclease, Pancreatic / Q000494:pharmacology; D013379:Substrate Specificity; D013696:Temperature
D012313:RNA; D004247:DNA; D004253:DNA Nucleotidylexotransferase; D012259:Ribonuclease, Pancreatic