Coordinate induction of metallothioneins I and II in rodent cells by UV irradiation.
Sequence analysis of Chinese hamster V79 lung fibroblast cDNA clones, which code for UV radiation-inducible transcripts, revealed that many of the clones corresponded to metallothioneins (MTs) I and II. A third cDNA clone, DDIU4, was found also to code for a similar-size UV-inducible transcript which was unrelated to MT by both sequence analysis and kinetics of induction. MTI and MTII RNAs rapidly increased in V79 cells within 1 h after UV irradiation, and maximum induction was seen by 4 h. This rapid induction of MT RNA by UV irradiation was not observed in human fibroblasts. MTI and MTII were coordinately induced in both time course and dose-response experiments, although the induction of MTII, up to 30-fold, was three to four times greater than that of MTI. The induction of MT did not appear to be a general stress response, since no increase occurred after exposure to X rays or H2O2.
Fornace
A J
AJ
Radiation Oncology Branch, National Cancer Institute, Bethesda, Maryland 20892.
Schalch
H
H
Alamo
I
I
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000235:genetics; D004249:DNA Damage; D007700:Kinetics; D008668:Metallothionein / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D012313:RNA / Q000096:biosynthesis / Q000235:genetics / Q000528:radiation effects; D014466:Ultraviolet Rays
D012313:RNA; D004247:DNA; D008668:Metallothionein