Production and characterisation of a monoclonal antibody to a cell-surface, glucomannoprotein constituent of Candida albicans and other pathogenic Candida species.
A murine monoclonal antibody (MAB AF-1) class IgM was raised to a soluble glucomannoprotein extract (GMP) of Candida albicans. Agglutination and indirect immunofluorescence assays with purified MAB showed that AF-1 was directed against a cell-surface epitope shared by C. albicans serotypes A and B, C. tropicalis, C. guilliermondii and C. viswanathii, but not by C. krusei, C. parapsilosis, C. kefyr (pseudotropicalis) and T. glabrata. Treatment with heat, protease or periodate-treated GMP and with other cell-wall extracts of C. albicans provided evidence that the epitope recognised by MAB AF-1 is carried by the polysaccharide moiety of cell-surface glucomannoprotein molecule(s).
Cassone
A
A
Medical Mycology Unit, Istituto Superiore di Sanità, Rome, Italy.
Torosantucci
A
A
Boccanera
M
M
Pellegrini
G
G
Palma
C
C
Malavasi
F
F
D000372:Agglutination Tests; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D000918:Antibody Specificity; D000946:Antigens, Fungal / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D002175:Candida / Q000276:immunology* / Q000648:ultrastructure; D002176:Candida albicans / Q000276:immunology* / Q000648:ultrastructure; D002473:Cell Wall / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D006825:Hybridomas; D005779:Immunodiffusion; D007075:Immunoglobulin M / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification; D008351:Mannans / Q000276:immunology; D008562:Membrane Glycoproteins; D051379:Mice
D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal; D000954:Antigens, Surface; D000939:Epitopes; D006023:Glycoproteins; D007075:Immunoglobulin M; D008351:Mannans; D008562:Membrane Glycoproteins; C025363:mannoproteins