Response of synovial fluid T cell clones to Yersinia enterocolitica antigens in patients with reactive Yersinia arthritis.
From synovial fluids of two patients with reactive arthritis following Yersinia enterocolitica infection, T lymphocyte clones were obtained that showed proliferative responses to Y. enterocolitica. The responses required autologous T-cell-depleted peripheral blood mononuclear cells as antigen presenting cells. Three clones were studied in detail; two of them showed a marked and specific response to Yersinia antigens alone, the other one recognized both Yersinia and Salmonella typhimurium antigens. The antigen-specific proliferation of the clones could be completely blocked by monoclonal antibodies to HLA-DR. These experiments show that synovial T lymphocytes presumably involved in the in situ immune response to microbial antigens triggering reactive arthritis can be cloned directly from the site of inflammation.
Hermann
E
E
I. Medizinische Klinik und Poliklinik, Johannes Gutenberg Universität, Mainz, FRG.
Fleischer
B
B
Mayet
W J
WJ
Poralla
T
T
Meyer zum Büschenfelde
K H
KH
D000328:Adult; D000942:Antigens, Bacterial / Q000276:immunology*; D001170:Arthritis, Infectious / Q000276:immunology*; D002999:Clone Cells; D000939:Epitopes / Q000276:immunology; D005260:Female; D006801:Humans; D007719:Knee Joint; D008213:Lymphocyte Activation; D008297:Male; D013582:Synovial Fluid / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D015009:Yersinia Infections / Q000276:immunology*; D015008:Yersinia enterocolitica / Q000276:immunology
D000942:Antigens, Bacterial; D000939:Epitopes