Accumulation of low density lipoproteins in stimulated rat serosal mast cells during recovery from degranulation.
Stimulation of rat serosal mast cells in vitro with compound 48/80, a degranulating agent, resulted in an immediate increase in binding of low density lipoproteins (LDL) to the stimulated mast cells. The increase in binding was dose-dependent and closely followed the increase in histamine release, i.e., the exocytosis of mast cell granules. It could be demonstrated that the LDL were bound to exocytosed secretory granules which remained cell-associated. During the recovery period the granule-bound LDL were internalized by the mast cells along with the granules. A single stimulation of mast cells rendered their cytoplasm to be filled with granular material showing positive staining for both apoB and neutral lipid. This change was accompanied by a 30-fold increase in the cellular content of cholesteryl esters. Thus, rat serosal mast cells possess a specific mechanism for uptake of LDL that is activated by stimuli that lead to degranulation, the result being massive uptake of LDL by stimulated mast cells during recovery from degranulation.
Kokkonen
J O
JO
Wihuri Research Institute, Helsinki, Finland.
Kovanen
P T
PT
D000818:Animals; D001161:Arteriosclerosis / Q000209:etiology; D001693:Biological Transport, Active; D003594:Cytoplasmic Granules / Q000378:metabolism; D005089:Exocytosis; D006636:Histamine Release / Q000187:drug effects; D066298:In Vitro Techniques; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D008077:Lipoproteins, LDL; D003189:p-Methoxy-N-methylphenethylamine