[An immunoenzyme method for determining the O-antigen of Sonne's shigella with the use of affinity isolated antibodies].
The sensitivities of ELISA systems for assays of Shigella sonnei O-antigen, developed on the basis of specific rabbit antibodies isolated from antisera by different chromatography techniques have been compared. The highest specificity and sensitivity of O-antigen determination (0.5-1 ng/ml) is achieved when using affinity-isolated antibodies to O-antigen. Clinical trials of this ELISA system have been carried out.
Voronov
A V
AV
Malov
V A
VA
Pak
S G
SG
Tur'ianov
M Kh
MKh
Gurskiĭ
Iu N
IuN
Serebriakov
M Iu
MIu
Gramotkina
E A
EA
Bengerov
Iu Ia
IuIa
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000942:Antigens, Bacterial / Q000032:analysis*; D002846:Chromatography, Affinity; D004405:Dysentery, Bacillary / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D019081:O Antigens; D011817:Rabbits; D012764:Shigella sonnei / Q000276:immunology*
D000906:Antibodies; D000942:Antigens, Bacterial; D019081:O Antigens
Immunofermentnyĭ metod opredeleniia O-antigena shigell Zonne s ispol'zovaniem affinno vydelennykh antitel.