Expression of B220 antigen on an interleukin 4- and interleukin 5-producing T-cell line.
The murine T-cell line 2.19, originally used for cloning of interleukin 4 (IL-4) and IL-5, was analysed for surface marker characteristics using monoclonal antibodies and a FACS-4 analyser and found to be positive for the B220 antigen. Thus, this lymphokine-producing cell with characteristic T-cell markers expresses an antigen earlier thought to be specific for B cells. With two-colour fluorescence it was found that a small fraction (2-4%) of Thy 1+ spleen cells from normal CBA mice were also positive for B220.
Sander
B
B
Department of Immunology, Stockholm University, Sweden.
Sideras
P
P
Möller
E
E
D000818:Animals; D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis; D002460:Cell Line; D006649:Histocompatibility Antigens / Q000032:analysis*; D007378:Interleukins / Q000096:biosynthesis*; D007700:Kinetics; D017493:Leukocyte Common Antigens; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D010641:Phenotype; D013154:Spleen / Q000166:cytology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology / Q000378:metabolism*; D018800:Thy-1 Antigens
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D000954:Antigens, Surface; D006649:Histocompatibility Antigens; D007378:Interleukins; D018800:Thy-1 Antigens; D017493:Leukocyte Common Antigens