Suppression of erythroid cell differentiation in mouse embryos exposed to retinoic acid in utero.
Differentiation of circulating erythrocytes was inhibited by all-trans-retinoic acid in 8- and 9-day-old mouse embryos exposed in utero on day 8 of gestation. Histochemical, light, and electron microscopic examinations revealed that all-trans-retinoic acid first decreased the numbers of polychromatic erythroblasts and then increased them. Simultaneously, immature erythroblasts proliferated, and differentiation of mature primitive erythrocytes was suppressed. Electron microscopy of these immature erythroblasts revealed monosomes rather than the usual polyribosomes in the cytoplasm. RNA histochemistry revealed a greater number of intermediate differentiating erythroblasts with pyronin-positive cytoplasm than those in the controls and revealed also a dose-dependent relationship between the number of polychromatic erythroblasts with positive pyronin staining and the controls. These findings suggest that all-trans-retinoic acid destroys the messenger RNA system, resulting in an inability to produce hemoglobin.
Yasuda
Y
Y
Department of Anatomy (1st Division), Kinki University School of Medicine, Osaka, Japan.
Konishi
H
H
Matsuo
T
T
Tanimura
T
T
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D004912:Erythrocytes / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D004920:Erythropoiesis / Q000187:drug effects; D005260:Female; D006651:Histocytochemistry; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008854:Microscopy, Electron; D012313:RNA / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology*; D015017:Yolk Sac / Q000166:cytology
D014212:Tretinoin; D012313:RNA