title: Sequence-specific isotope effects on the cleavage of DNA by bleomycin.
abstract: Bleomycin is a metal- and oxygen-dependent DNA cleaver. The chemistry of DNA damage has been proposed to involve rate-limiting abstraction of the 4'-hydrogen. A DNA fragment has been prepared that contains [4'-2H]thymidine residues of high isotopic content. Primary kinetic isotope effects have been directly observed at individual thymidine residues with DNA sequencing technology.
authors: Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742.
authors: J W
authors: JW
authors: Kozarich
authors: L
authors: L
authors: Worth
authors: B L
authors: BL
authors: Frank
authors: D F
authors: DF
authors: Christner
authors: D E
authors: DE
authors: Vanderwall
authors: J
authors: J
authors: Stubbe
mesh_terms: D001483:Base Sequence; D001761:Bleomycin; D004249:DNA Damage; D003903:Deuterium; D007501:Iron; D010100:Oxygen; D013329:Structure-Activity Relationship; D013936:Thymidine
chemical_list: D001761:Bleomycin; D003903:Deuterium; D007501:Iron; D010100:Oxygen; D013936:Thymidine