title: Diagnosis of renal allograft rejection by macrophage immunostaining with a CD14 monoclonal antibody, WT14.
abstract: Since acute interstitial rejection (AIR) of renal allografts is accompanied by an increase of macrophages in the graft the diagnostic value of immunohistological staining of biopsy specimens with WT14, a new monoclonal antibody of the CD14 cluster directed against monocytes/macrophages, with increased affinity for activated cells, has been tested retrospectively. With an indirect immunoperoxidase technique on frozen sections a diffuse interstitial increase of WT14-positive cells was seen, with a characteristic peritubular pattern, in all 44 patients with clinically and histologically proven AIR. This pattern was not seen in normal kidneys (n = 10), or in biopsy specimens from patients with proven cyclosporin nephrotoxicity (n = 9), chronic vascular rejection (n = 13), or various other renal diseases (n = 60). Comparative staining with other monoclonal antibodies against monocytes/macrophages showed a variable, mostly weak or less specific staining pattern than did WT14. The increased staining with WT14 proved to be a better indicator of AIR than increased HLA-DR staining on tubular epithelial cells.
authors: Department of Pathology, University Hospital Nijmegen, The Netherlands.
authors: M J
authors: MJ
authors: Bogman
authors: I M
authors: IM
authors: Dooper
authors: J G
authors: JG
authors: van de Winkel
authors: W J
authors: WJ
authors: Tax
authors: A J
authors: AJ
authors: Hoitsma
authors: K J
authors: KJ
authors: Assmann
authors: D J
authors: DJ
authors: Ruiter
authors: R A
authors: RA
authors: Koene
mesh_terms: D000911:Antibodies, Monoclonal; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology*; D003524:Cyclosporins / Q000009:adverse effects; D006084:Graft Rejection / Q000187:drug effects; D006801:Humans; D007668:Kidney / Q000473:pathology; D007674:Kidney Diseases / Q000139:chemically induced / Q000473:pathology; D016030:Kidney Transplantation; D018950:Lipopolysaccharide Receptors; D008264:Macrophages / Q000473:pathology*; D013194:Staining and Labeling
chemical_list: D000911:Antibodies, Monoclonal; D015214:Antigens, Differentiation, Myelomonocytic; D003524:Cyclosporins; D018950:Lipopolysaccharide Receptors