Methotrexate suppresses passive adjuvant arthritis: studies on the metabolism of methotrexate in mononuclear cells derived from normal and adjuvant arthritic rats.
Passive transfer of adjuvant arthritis by spleen cells is suppressed by methotrexate. Mononuclear cells derived from spleens of normal and adjuvant arthritic Lewis rats were incubated with [3H]-methotrexate and harvested at various periods of time. The amount of methotrexate and its various polyglutamates were quantitated. The results of these studies indicate that the kinetics of uptake of methotrexate by mononuclear cells from normal and adjuvant arthritic rats are similar. However, the amount of methotrexate polyglutamates accumulating in the mononuclear cells of adjuvant arthritic rats was significantly lower than that observed in mononuclear cells derived from normal rats.
Ridge
S C
SC
Department of Inflammation and Immunology, American Cyanamid Company, Lederle Laboratories, Pearl River, NY 10965.
Ferguson
K M
KM
Rath
N
N
Galivan
J
J
Freisheim
J H
JH
Oronsky
A L
AL
Kerwar
S S
SS
D000818:Animals; D001168:Arthritis / Q000188:drug therapy*; D001169:Arthritis, Experimental / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D002469:Cell Separation; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008727:Methotrexate / Q000031:analogs & derivatives / Q000378:metabolism / Q000493:pharmacokinetics / Q000627:therapeutic use*; D011099:Polyglutamic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D051381:Rats; D011917:Rats, Inbred Lew; D012016:Reference Values; D013154:Spleen / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D013997:Time Factors
D011099:Polyglutamic Acid; C014085:methotrexate polyglutamate; D008727:Methotrexate