Serum protein leakage in aged human brain and inhibition of ligand binding at alpha 2-adrenergic and cholinergic binding sites.
Serum proteins are known to extravasate into the brain parenchyma in senile and presenile dementia (Glenner: Hum. Pathol. 16:433-435, 1986; Wisniewski and Kozlowski: Ann. NY Acad. Sci. 396:119-129, 1982). We have recently demonstrated that human serum Cohn fraction IV (alpha-globulin enriched) inhibits ligand binding at putative dopamine and serotonin2 receptors labeled by [3H]spiroperidol in human brain (Andorn, Pappolla, Fox, Klemens, and Martello: Proc. Natl. Acad. Sci. USA 83:4572-4575, 1986). We now demonstrate that serum proteins can be identified in the neuropil and in neuronal cell bodies in normal aged brain, that alpha-globulin-enriched fractions inhibit ligand binding at alpha 2-adrenergic and muscarinic binding sites in human brain as well, and that serum proteins can be identified within neuronal cytoplasm and axons.
Pappolla
M A
MA
Department of Pathology, New York Medical College, New York.
Andorn
A C
AC
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging; D000510:Alpha-Globulins / Q000032:analysis; D001798:Blood Proteins / Q000378:metabolism / Q000502:physiology*; D001812:Blood-Brain Barrier; D001921:Brain / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007136:Immunoglobulins / Q000032:analysis; D008854:Microscopy, Electron; D008875:Middle Aged; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism*; D011950:Receptors, Cholinergic / Q000378:metabolism*; D000511:alpha-Macroglobulins / Q000032:analysis
D000510:Alpha-Globulins; D001798:Blood Proteins; D007074:Immunoglobulin G; D007136:Immunoglobulins; D011942:Receptors, Adrenergic, alpha; D011950:Receptors, Cholinergic; D000511:alpha-Macroglobulins