title: Recent advances in brain-gut hormones.
abstract: Although recent advances in gene technology have made it possible to produce peptides and proteins by the recombinant technique, chemical synthesis either by the solution or by the solid-phase technique is still only a method of choice for the syntheses of a brain-gut hormone and its superagonist and/or antagonist. In addition, synthetic replicates of brain-gut hormones, their analogus and fragments or their precursor-related peptides are important haptenic immunogens to produce region-specific antisera to the respective peptides. In the present study, antisera against proglucagon and pro-LH-RH related peptides were proved to be useful not only for identifying hormone-producing cells, but also for demonstrating the post-translational biosynthetic processing in the cells. For galanin, a novel brain-gut hormone, have structural similarity in its C-terminal region to tachykinins, a monoclonal antibody against synthetic porcine galanin (1-15) was prepared for immunohistochemical study. This monoclonal antibody was found to recognize specifically galanin-producing cells and not to cross-react with any of tachykinins examined. These observations indicate that monoclonal antibodies are highly useful for identifying cells possessing a brain-gut hormone containing structures common to those of different known peptides.
authors: Laboratory of Bioorganic Chemistry, University of Shizuoka School of Pharmaceutical Sciences, Japan.
authors: N
authors: N
authors: Yanaihara
authors: C
authors: C
authors: Yanaihara
authors: T
authors: T
authors: Zhang
authors: M
authors: M
authors: Hoshino
authors: K
authors: K
authors: Iguchi
authors: T
authors: T
authors: Mochizuki
mesh_terms: D000818:Animals; D019004:Galanin; D005934:Glucagon / Q000378:metabolism*; D004763:Glucagon-Like Peptides; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D010455:Peptides / Q000032:analysis / Q000378:metabolism*; D052336:Proglucagon; D011498:Protein Precursors / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D011995:Recombination, Genetic
chemical_list: D010455:Peptides; D011498:Protein Precursors; D011994:Recombinant Proteins; D007987:Gonadotropin-Releasing Hormone; D052336:Proglucagon; D004763:Glucagon-Like Peptides; C015236:glucagon-like-immunoreactivity; D019004:Galanin; D005934:Glucagon