In situ hybridization technique using an immunogold silver staining system.
An in situ hybridization technique using an immunogold silver staining detection system was used to detect biotinylated DNA probes in cultured cells and in formalin-fixed paraffin-embedded tissue. The detection method is rapid, reliable and economical, producing an insoluble signal at the site of hybridization which is visible at low-power light microscopy and which can be enhanced by epipolarization microscopy.
Jackson
P
P
Department of Pathology, University of Leeds, UK.
Lewis
F A
FA
Wells
M
M
D002460:Cell Line; D004247:DNA / Q000032:analysis*; D015342:DNA Probes; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D009693:Nucleic Acid Hybridization; D012834:Silver; D013194:Staining and Labeling
D015342:DNA Probes; D012834:Silver; D004247:DNA