[New application of monoclonal antibody for cancer diagnosis].
Clinical significance of serodiagnosis of adenocarcinoma-associated antigen which were detected in our laboratory was discussed. Anti-idiotype monoclonal antibody against monoclonal antibody YH206 was prepared and found useful for the detection of anti-YH206 in patient sera. The simultaneous measurement of anti-YH206 antibody and YH206 antigen is of value and a new application of anti-idiotype antibody to the cancer diagnosis. The third point of the report was a result on mRNA expression in cancerous and non-cancerous tissues by Northern blotting method using CEA- and NCA-cDNA probes. Finally, it was reported that we prepared monoclonal antibodies to synthetic peptide which was identical to a part of CEA domain I (AAs 181-194). The reactivities were characterized and the usefulness to a detailed analysis of CEA epitope and the possible application to serodiagnosis were discussed.
Yachi
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A
Dept. of Internal Medicine, Sapporo Medical College.
D000230:Adenocarcinoma / Q000276:immunology; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis / Q000276:immunology; D015152:Blotting, Northern; D002272:Carcinoembryonic Antigen / Q000235:genetics / Q000276:immunology; D015342:DNA Probes; D000939:Epitopes / Q000032:analysis; D006801:Humans; D007130:Immunoglobulin Idiotypes / Q000032:analysis / Q000276:immunology; D009369:Neoplasms / Q000175:diagnosis* / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D002272:Carcinoembryonic Antigen; D015342:DNA Probes; D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D012333:RNA, Messenger