Production of inositol trisphosphate isomers and release of intracellular calcium in cultured aortic vascular smooth muscle cells.
Two early steps of angiotensin II-induced biological response have been examined in cultured rat aortic, smooth muscle cells: (a) In addition to rapidly raising inositol 1,4,5-trisphosphate production, angiotensin II also induced the appearance of the isomer, inositol 1,3,4-trisphosphate, with similar kinetics: (b) Inositol 1,4,5-trisphosphate released calcium from an intracellular pool in electropermeabilized smooth muscle cells, in a concentration-dependent manner (EC50 = 0.25 microM). These data reinforce the concept of a critical role for agonist-induced polyphosphoinositide hydrolysis in the calcium-mediated responses of smooth muscle cells to angiotensin II.
Capponi
A M
AM
Division of Endocrinology, University Hospital, Geneva, Switzerland.
Rossier
M F
MF
Vallotton
M B
MB
D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D001013:Aorta, Thoracic / Q000166:cytology / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D003600:Cytosol / Q000378:metabolism; D066298:In Vitro Techniques; D007292:Inosine Nucleotides / Q000096:biosynthesis*; D007293:Inosine Triphosphate / Q000096:biosynthesis*; D007536:Isomerism; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000378:metabolism*; D051381:Rats
D007292:Inosine Nucleotides; D000804:Angiotensin II; D007293:Inosine Triphosphate; D002118:Calcium