title: Effects of increased major histocompatibility complex dosage on chicken monocyte-macrophage function.
abstract: The influence of major histocompatibility complex (B complex) dosage on monocyte-macrophage function was examined using 4- to 6-week-old trisomic strain chickens. Di- (B15B15), tri- (B15B15B15), and tetrasomic (B15B15B15B15) progeny were produced from trisomic x trisomic crosses. Although mononuclear leukocytes from tetrasomics exhibited enhanced chemotactic activity in response to both f-met-leu-phe and Enterobacter cloacae culture supernatant as compared with that of cells from other groups, the ability to generate peritoneal exudate cells in response to intraperitoneal Sephadex stimulation was similar in all groups. Among peritoneal exudate cells, tetrasomic birds produced a significantly lower percentage of adherent macrophages with a higher proportion of Fc receptor-positive and CMTD-2-reactive macrophages than either disomic or trisomic chickens. Both tetrasomic and trisomic peritoneal macrophages exhibited a reduced phagocytic activity for unopsonized but not opsonized SRBC than was found with disomic macrophages. Thus, the number of major histocompatibility complex copies present in cells appears to influence monocyte-macrophage function.
authors: Department of Poultry and Avian Sciences, Cornell University, Ithaca, New York 14853.
authors: M A
authors: MA
authors: Qureshi
authors: S E
authors: SE
authors: Bloom
authors: R R
authors: RR
authors: Dietert
mesh_terms: D000782:Aneuploidy; D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002448:Cell Adhesion; D002634:Chemotaxis, Leukocyte; D002645:Chickens; D003911:Dextrans / Q000494:pharmacology; D004303:Dosage Compensation, Genetic; D004912:Erythrocytes; D005898:Glass; D007963:Leukocytes, Mononuclear / Q000502:physiology*; D008264:Macrophages / Q000502:physiology*; D008285:Major Histocompatibility Complex; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009895:Opsonin Proteins; D010587:Phagocytosis; D011961:Receptors, Fc / Q000378:metabolism; D012756:Sheep; D014314:Trisomy
chemical_list: D003911:Dextrans; D009895:Opsonin Proteins; D011961:Receptors, Fc; D009240:N-Formylmethionine Leucyl-Phenylalanine; C025614:sephadex