The application of immunoperoxidase staining for the detection of causative fungi in tissue specimens of mycosis I.
This study was performed in order to identify the fungi of four species (Aspergillus fumigatus, Fusarium anthophilum, Candida albicans, Cryptococcus neoformans) in formalin-fixed, paraffin-embedded tissue sections by the indirect method of immunoperoxidase staining. Mature albino rabbits were immunized by formalin-killed organisms. The antibodies were prepared by precipitation at a 50% saturation of ammonium sulfate and were checked for cross-reactivities by Ouchterlony's double immunodiffusion and precipitin test. The immunoperoxidase staining was applied to the paraffin-embedded tissue sections of infected mice, human autopsy and biopsy specimens. Although each fungus was stained clearly the cell wall, cross-reactivities appeared among them, however it was possible to identify four fungi by absorption and dilution of the antisera.
Kobayashi
K
K
Department of Plastic Surgery, Nagasaki University School of Medicine, Japan.
Hayama
M
M
Hotchi
M
M
D000818:Animals; D001228:Aspergillosis / Q000175:diagnosis; D001232:Aspergillus fumigatus; D002177:Candidiasis / Q000175:diagnosis; D003429:Cross Reactions; D003453:Cryptococcosis / Q000175:diagnosis; D005670:Fusarium; D007106:Immune Sera; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D051379:Mice; D009181:Mycoses / Q000175:diagnosis*; D011817:Rabbits / Q000276:immunology; D013194:Staining and Labeling
D007106:Immune Sera