Low-amplified N-myc gene and pp60src in retinoblastoma TOTL-1 cells which undergo neuron-like differentiation.
A cell line of retinoblastoma cells, TOTL-1, was established which had a very weakly amplified N-myc gene. It grew only in clumps that were hardly ever dispersed and showed retarded proliferation. Its doubling time was 160 h. TOTL-1 cells extended short thick processes in monolayer cultures on poly-D-lysine coated substratum, which were markedly different from those of Y79 cells. Light and electron microscopy observation of immunostaining with anti-pp60src antibody revealed that the staining was accumulated in the polar regions of the cells.
Wakabayashi
K
K
Department of Biochemistry, Yamanashi Medical College, Japan.
Atsumi
S
S
Sasabe
T
T
Mori
S
S
D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D003593:Cytoplasm / Q000648:ultrastructure; D005134:Eye Neoplasms / Q000235:genetics* / Q000473:pathology / Q000648:ultrastructure; D005784:Gene Amplification; D005786:Gene Expression Regulation; D006801:Humans; D008854:Microscopy, Electron; D015688:Oncogene Protein pp60(v-src); D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc; D016392:Proto-Oncogene Proteins pp60(c-src); D012175:Retinoblastoma / Q000235:genetics* / Q000473:pathology / Q000648:ultrastructure; D012191:Retroviridae Proteins; D013194:Staining and Labeling / Q000379:methods; D014407:Tumor Cells, Cultured / Q000473:pathology / Q000648:ultrastructure
D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D012191:Retroviridae Proteins; D015688:Oncogene Protein pp60(v-src); D016392:Proto-Oncogene Proteins pp60(c-src)