title: An analysis of total RNA translation products of rat liver during regeneration with a comparison to fetal liver.
abstract: We explored differences in the mRNA populations of regenerating, sham-operated, and fetal rat liver using two-dimensional gel electrophoresis to resolve the radiolabeled protein products of liver total RNA translated in vitro. Twenty-four translation products were changed significantly after partial hepatectomy and sham hepatectomy. Nine of the 24 products changed after partial hepatectomy only, while the remainder also changed after sham hepatectomy. Two of the nine increased during regeneration to relative levels similar to those found in fetal liver. However, substantial differences also exist between the translation products of regenerating and fetal liver. These results suggest that the response to partial hepatectomy involves the alteration of mRNAs present in normal liver and that this response does not duplicate the fetal pattern of hepatic total RNA translation activity.
authors: Department of Medicine, Hennepin County Medical Center, Minneapolis, Minnesota 55415.
authors: H S
authors: HS
authors: Beyer
authors: B M
authors: BM
authors: Sharp
authors: L
authors: L
authors: Zieve
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005333:Fetus / Q000032:analysis* / Q000378:metabolism / Q000502:physiology; D006498:Hepatectomy; D007668:Kidney / Q000032:analysis / Q000378:metabolism; D008099:Liver / Q000032:analysis* / Q000196:embryology / Q000502:physiology; D008115:Liver Regeneration / Q000235:genetics* / Q000502:physiology; D008297:Male; D009132:Muscles / Q000032:analysis / Q000378:metabolism; D014176:Protein Biosynthesis / Q000235:genetics* / Q000502:physiology; D012313:RNA / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D012333:RNA, Messenger; D012313:RNA