Review of selected lineage-directed antibodies useful in routinely processed tissues.
This review discusses several commonly used lineage-directed antibodies, including those to cytokeratins, vimentin, glial fibrillary acidic protein, neurofilaments, desmin, actin, leukocyte common antigen, and melanoma-specific antigen. Discussion is particularly directed to issues of antibody sensitivity and specificity, with several confusing and/or potentially beneficial examples of cross-lineage antigenic co-expression cited. The value of using limited panels composed of antibodies with complementary specificities is also emphasized.
Corwin
D J
DJ
Department of Pathology, University of Washington, Seattle.
Gown
A M
AM
D000199:Actins / Q000276:immunology; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D000943:Antigens, Differentiation / Q000276:immunology; D002460:Cell Line; D003599:Cytoskeleton / Q000276:immunology; D003893:Desmin / Q000276:immunology; D005904:Glial Fibrillary Acidic Protein / Q000276:immunology; D006649:Histocompatibility Antigens / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D007382:Intermediate Filaments / Q000276:immunology; D007633:Keratins / Q000276:immunology; D017493:Leukocyte Common Antigens; D008545:Melanoma / Q000276:immunology; D014746:Vimentin / Q000276:immunology
D000199:Actins; D000906:Antibodies; D000943:Antigens, Differentiation; D003893:Desmin; D005904:Glial Fibrillary Acidic Protein; D006649:Histocompatibility Antigens; D014746:Vimentin; D007633:Keratins; D017493:Leukocyte Common Antigens