Supravital microscopic fluorescent technique for the detection of tinea capitis.
A simple one-step, office technique for the identification of superficial mycoses is presented. Buffered acridine orange is used as a supravital stain for direct examination of specimens under a fluorescence microscope. Neither prior treatment with potassium hydroxide, nor tissue fixation is required. Results usually can be obtained in less than five minutes. Extensive training in use of the microscope is not required.
Reque
P G
PG
Department of Dermatology, University of Alabama, Birmingham.
Lorincz
A E
AE
D000165:Acridine Orange; D006197:Hair / Q000382:microbiology; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D008864:Microsporum / Q000302:isolation & purification; D013194:Staining and Labeling; D014006:Tinea Capitis / Q000175:diagnosis* / Q000382:microbiology; D014249:Trichophyton / Q000302:isolation & purification
D000165:Acridine Orange