title: Activation of murine B cells with Salmonella typhimurium mitogen (STM), lipopolysaccharide (LPS), and dextran sulfate (DxS). I. Cell-cycle analysis and induction of cytoplasmic immunoglobulin.
abstract: There have been two recent reports concerning a B cell-specific mitogen that induces proliferation, but not differentiation of rat and human B cells. This mitogen, which is derived from Salmonella typhimurium (STM), appears to be providing the signals required for anti-immunoglobulin-treated (anti-Ig) B cells to enter cycle and divide, but may not be inducing responsiveness to B cell differentiation factors (BCDF). In this report, we have compared STM to the other known murine B cell polyclonal activators: lipopolysaccharide (LPS), dextran sulfate (DxS), and the combination of LPS/DxS. STM was the most potent stimulus of B cell proliferation as determined by uptake of 3H-thymidine, viable cell numbers and cell cycle analysis utilizing acridine orange (AO). STM did not induce significant proliferation of murine T lymphocytes. In addition, the proliferative effect of STM on B cells shows minimal, if any, macrophage dependence. However, in contrast to its effect on human and rat B cells, STM induces differentiation of murine B cells. The levels of cytoplasmic Ig induced by STM are equivalent or greater to those induced by LPS/DxS. Thus, in the murine system, STM will be useful as a polyclonal activator which induces proliferation and differentiation of the vast majority of the B cell population without stringent accessory cell requirements.
authors: Department of Microbiology, University of Texas Health Science Center, Southwestern Medical School, Dallas 75235.
authors: K H
authors: KH
authors: Brooks
authors: E S
authors: ES
authors: Vitetta
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002453:Cell Cycle; D003593:Cytoplasm / Q000276:immunology; D016264:Dextran Sulfate; D003911:Dextrans / Q000494:pharmacology; D005260:Female; D007136:Immunoglobulins / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D051379:Mice; D008934:Mitogens / Q000494:pharmacology; D012486:Salmonella typhimurium / Q000276:immunology
chemical_list: D003911:Dextrans; D007136:Immunoglobulins; D008070:Lipopolysaccharides; D008934:Mitogens; D016264:Dextran Sulfate