Preliminary comparisons of the biological properties of two strains of feline immunodeficiency virus (FIV) isolated in Japan with FIV Petaluma strain isolated in the United States.
Two strains of feline immunodeficiency virus (FIV) were isolated directly from peripheral blood mononuclear cells (PBMCs) of Japanese domestic cats or indirectly from PBMCs of specific pathogen free (SPF) cats inoculated with whole blood from naturally infected cats with FIV by cocultivation with primary PBMCs from SPF cats. Two isolates, designated as FIV TM 1 and FIV TM 2, had a lentivirus-like morphology by electron microscopy, a tropism for interleukin-2 dependent T-lymphocytes and Mg2+-dependent reverse transcriptase activity. By immunoblotting the isolates gave bands at 130, 48, 44, 40, 28, 17, and 13 kDa, and these bands except 130 kDa were detected in FIV Petaluma strain when reacted with the plasma of cats infected naturally with FIV TM 1 strain.
Miyazawa
T
T
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Furuya
T
T
Itagaki
S
S
Tohya
Y
Y
Nakano
K
K
Takahashi
E
E
Mikami
T
T
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D015153:Blotting, Western; D002371:Cat Diseases / Q000382:microbiology*; D002415:Cats / Q000382:microbiology*; D005260:Female; D007564:Japan; D007963:Leukocytes, Mononuclear / Q000382:microbiology; D008297:Male; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012190:Retroviridae / Q000145:classification / Q000302:isolation & purification* / Q000648:ultrastructure; D012192:Retroviridae Infections / Q000382:microbiology / Q000662:veterinary*; D013045:Species Specificity; D013047:Specific Pathogen-Free Organisms; D014481:United States; D014764:Viral Proteins / Q000032:analysis
D000956:Antigens, Viral; D014764:Viral Proteins; D012194:RNA-Directed DNA Polymerase