The activity of bleomycin and modifiers of its clastogenic effect on the interphase chromatin of Indian muntjak fibroblasts.
Premature chromosome condensation was induced in Indian muntjak fibroblasts after exposure of the cells to bleomycin. Further experiments were devoted to the interaction of anticlastogens and a repair inhibitor, streptovitacin A. Chromosomal aberrations due to bleomycin treatment were S-phase-independently visible in the G1 and G2 phase of the cell cycle. For premature chromosome condensation experiments, a 100-fold lower concentration of the mutagen produced a similar extent of chromosome damage as in metaphase studies. Additional exposure to the anticlastogens beta-aminoethylisothiouronium or N-acetylcysteine revealed differences between corresponding interphase and metaphase effects and between different exposure conditions. Streptovitacin A, known as an inhibitor of protein synthesis, acted like an anticlastogen in the G2 phase of the cell cycle. Our studies show that the premature chromosome condensation technique offers various qualitative insights into primary processes of mutagenicity and antimutagenicity, but requires further improvement and careful choice of the cell system for study.
Tittelbach
H
H
Institute of Human Genetics, University of Erlangen-Nurnberg, Federal Republic of Germany.
Brüderlein
S
S
Gebhart
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E
D000111:Acetylcysteine / Q000494:pharmacology; D000818:Animals; D001761:Bleomycin / Q000037:antagonists & inhibitors / Q000633:toxicity*; D002460:Cell Line; D002843:Chromatin / Q000187:drug effects*; D002869:Chromosome Aberrations; D003513:Cycloheximide / Q000031:analogs & derivatives / Q000633:toxicity; D003670:Deer; D005347:Fibroblasts; D007399:Interphase / Q000187:drug effects*; D008297:Male; D008677:Metaphase / Q000187:drug effects; D009153:Mutagens; D000338:beta-Aminoethyl Isothiourea / Q000494:pharmacology
D002843:Chromatin; D009153:Mutagens; D001761:Bleomycin; D000338:beta-Aminoethyl Isothiourea; C005257:streptovitacin A; D003513:Cycloheximide; D000111:Acetylcysteine