[Cytolysin as an immunoserological marker of Legionella pneumophila].
Coagglutination test (CoaT), radioimmunoassay (RIA) and ELISA were used for detection of cytolysin produced by L. pneumophila. The sensitivity of RIA was 100 ng/ml, CoaT--10-20 ng/ml, ELISA--1 ng/ml. To determine cytolysin production among various strains and species of Legionella, the authors studied bacterial ultrasonic lysates. All 11 strains of L. pneumophila tested were cytolysin-positive. L. longbeachae, L. bozemanii and L. dumoffii strains failed to produce cytolysin. The authors believe that an antigen of cytolysin can be used for identification of L. pneumophila.
Tartakovskiĭ
I S
IS
Belyĭ
Iu F
IuF
Vertiev
Iu V
IuV
Nagaev
I G
IG
Prozorovskiĭ
S V
SV
D000372:Agglutination Tests / Q000379:methods; D000942:Antigens, Bacterial / Q000032:analysis; D001345:Autoradiography; D003603:Cytotoxins / Q000032:analysis* / Q000276:immunology; D000939:Epitopes / Q000032:analysis; D007124:Immunoenzyme Techniques; D007875:Legionella / Q000145:classification* / Q000276:immunology; D012703:Serotyping / Q000379:methods; D013045:Species Specificity
D000942:Antigens, Bacterial; D003603:Cytotoxins; D000939:Epitopes
Tsitolizin kak immunoserologicheskiĭ marker Legionella pneumophila.