Lack of immunodominance in the T cell response to herpes simplex virus glycoprotein D after administration of infectious virus.
Glycoprotein D (gD) of HSV has been shown to be a potent immunogen. To analyze the T cell antigenic determinants on gD, a series of 28 overlapping 20-mer peptides that span the extracellular portion of gD-1 were examined for their ability to stimulate T cells from rgD-1 or infectious HSV-1-primed H-2d mice in vitro. rgD-1-primed cells responded exclusively to peptide 241-260, the immunodominant determinant of gD in H-2d mice. In contrast, infectious HSV-primed T cells were shown to respond to 17 (and up to 22) of 28 synthetic gD peptides. These results indicate an extensive diversity in the T cell repertoire to gD in H-2d mice with T cells directed to a broad array of peptide determinants being recruited during the acute phase of an HSV infection.
Yamashita
K
K
Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104.
Heber-Katz
E
E
D000818:Animals; D000938:Antigen-Presenting Cells / Q000502:physiology; D000939:Epitopes / Q000032:analysis*; D006561:Herpes Simplex / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*
D000939:Epitopes; D000949:Histocompatibility Antigens Class II; D014759:Viral Envelope Proteins; C039447:glycoprotein D, Human herpesvirus 1