[Possibilities of using nuclear filters in microbiological studies].
The authors have examined the regularities of the staphylococcal culture filtration through nuclear membranes with pore diameters of up to 0.6 microns, as well as the possibility of sterilization and multiple use of these filters. S. aureus strain No. S. 593 isolated from pathological material has been used as the test bacterium. Routine milk-yolk salt agar and meat-peptone salt broth with 10% NaCl have been employed for the cultivation of the test strain. The filters could be fairly well sterilized with Nikiforov's mixture; then they were dried under a UV bactericidal lamp. The studies have demonstrated that staphylococci can pass through nuclear membranes with pores 0.6 microns in diameter. For sterilization of biologic media membranes with pore diameters of 0.1-0.3 microns are preferable.
Kapitonenko
N A
NA
Ivanov
N A
NA
Danilova
E G
EG
D008828:Microbiological Techniques; D008851:Micropore Filters
Izuchenie vozmozhnosti primeneniia iadernykh fil'trov v mikrobiologicheskikh issledovaniiakh.