The gene for human lymphocyte homing receptor is located on chromosome 11.
The mouse monoclonal antibody Hermes-3 recognizes the human lymphocyte homing receptor. A panel of mouse-human T lymphocyte hybrids, carrying all mouse chromosomes and a limited number of human chromosomes, was analyzed for expression of human homing receptor by indirect immunofluorescence and immunoprecipitation of radiolabeled cell lysates with Hermes-3 antibody. Karyotypic analysis of the tested clones showed that the expression of human homing receptor correlated to the presence of human chromosome 11 in all but one clone. However, concanavalin A induced a weak to moderate expression of the homing receptor in this clone, but not in a chromosome 11- clone. Another clone, heterogeneous for the expression of homing receptor, was separated into a Hermes-3+ and a Hermes-3- fraction with a fluorescence-activated cell sorter. Karyotypic analysis performed after sorting showed human chromosome 11 to segregate with the Hermes-3 antigen. To confirm these data we correlated the expression of two chromosome 11-coded antigens, Trop-4 and Leu-7, with the expression of the homing receptor. In our hybrid clones these three antigens were expressed concordantly. The gene coding for the human lymphocyte homing receptor recognized by Hermes-3 is thus assigned to chromosome 11.
Forsberg
U H
UH
Department of Genetics, University of Helsinki, Finland.
Ala-Kapee
M M
MM
Jalkanen
S
S
Andersson
L C
LC
Schröder
J
J
D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis; D018959:CD57 Antigens; D002460:Cell Line; D002880:Chromosomes, Human, Pair 11; D003208:Concanavalin A / Q000494:pharmacology; D005796:Genes; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008807:Mice, Inbred BALB C; D011233:Precipitin Tests; D011971:Receptors, Immunologic / Q000032:analysis / Q000235:genetics*; D016201:Receptors, Lymphocyte Homing; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*
D000943:Antigens, Differentiation; D018959:CD57 Antigens; D011971:Receptors, Immunologic; D016201:Receptors, Lymphocyte Homing; D003208:Concanavalin A