Triton X-100 extractions of central nervous system myelin indicate a possible role for the minor myelin proteins in the stability in lamellae.
Isolated CNS myelin membranes were extracted with Triton X-100 under conditions previously established for the isolation of cytoskeletal proteins. Treated myelin retained much of its characteristic lamellar structure despite the removal of most of the major myelin basic protein (18.5 kDa) and the proteolipid protein, which together normally constitute 60% of the total myelin protein. The SDS-PAGE profile of this extract residue demonstrated an enrichment in proteins of Mr 30 to 60 kilodaltons (the Wolfgram group). The major myelin proteins were identified by antibodies on Western immunoblots, as were the 2'3'-cyclic nucleotide 3'-phosphodiesterase (CNP), actin, tubulin, myelin-associated glycoprotein (MGP) and the 21.5 kDA MBP. The overall behavior of CNP, the 21.5 kDa MBP, MGP and tubulin towards Triton extraction is reminiscent of the behavior of other membrane-skeletal complexes, supporting the idea that these and other minor myelin proteins might be part of heteromolecular complexes with interactions spanning several lamellae of the myelin sheath.
Pereyra
P M
PM
McGill University, Dept. Biochemistry, Montreal, Quebec, Canada.
Horvath
E
E
Braun
P E
PE
D000818:Animals; D002417:Cattle; D002457:Cell Extracts; D003598:Cytoskeletal Proteins / Q000032:analysis; D051379:Mice; D008854:Microscopy, Electron; D008970:Molecular Weight; D004676:Myelin Basic Protein / Q000032:analysis*; D009185:Myelin Proteins / Q000032:analysis*; D009186:Myelin Sheath / Q000032:analysis* / Q000648:ultrastructure; D018992:Myelin-Associated Glycoprotein; D017830:Octoxynol; D010743:Phospholipids / Q000032:analysis; D011092:Polyethylene Glycols; D011817:Rabbits
D002457:Cell Extracts; D003598:Cytoskeletal Proteins; D004676:Myelin Basic Protein; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D010743:Phospholipids; D011092:Polyethylene Glycols; D017830:Octoxynol