title: Ferredoxin-NADP+ oxidoreductase is the respiratory NADPH dehydrogenase of the cyanobacterium Anabaena variabilis.
abstract: The NADPH dehydrogenase of the cyanobacterium Anabaena variabilis was solubilized, purified, and characterized. Activity staining after nondenaturing polyacrylamide gel electrophoresis, kinetics, and immunological characterization led to the conclusion that only one thylakoid-associated NADPH dehydrogenase exists in Anabaena, identical with ferredoxin-NADP+ oxidoreductase (FNR). After sodium dodecyl sulfate-polyacrylamide gel electrophoresis an intense band at 34 kDa and a weak band at 52 kDa were found by immunoblotting with an antibody against Anabaena FNR. Using a cell-free preparation competent of oxidative phosphorylation it was demonstrated that FNR operates as a respiratory NADPH dehydrogenase coupled to cyanide-sensitive oxidative ATP formation.
authors: Lehrstuhl für Physiologie und Biochemie der Pflanzen Universität Konstanz, West Germany.
authors: S
authors: S
authors: Scherer
authors: I
authors: I
authors: Alpes
authors: H
authors: H
authors: Sadowski
authors: P
authors: P
authors: Böger
mesh_terms: D000458:Cyanobacteria / Q000201:enzymology* / Q000502:physiology; D004579:Electron Transport; D005287:Ferredoxin-NADP Reductase / Q000502:physiology*; D009247:NADH, NADPH Oxidoreductases / Q000502:physiology*; D009252:NADPH Dehydrogenase / Q000502:physiology*; D010101:Oxygen Consumption; D010766:Phosphorylation; D012995:Solubility; D013194:Staining and Labeling
chemical_list: D005287:Ferredoxin-NADP Reductase; D009247:NADH, NADPH Oxidoreductases; D009252:NADPH Dehydrogenase