Chemical deglycosylation on a micro-scale of membrane glycoproteins with retention of phosphoryl-protein linkages.
We provide here an easy and convenient procedure for the chemical deglycosylation of membrane glycoproteins that can be done rapidly and inexpensively on large numbers of membrane samples on a microscale without prior isolation of the glycoprotein in question. We have used the procedure successfully on three different glycoproteins and additionally have shown that covalent linkages of the polypeptide to phosphoryl residues are preserved.
Horvath
E
E
McGill University, Department of Biochemistry, Montreal, Quebec, Canada.
Edwards
A M
AM
Bell
J C
JC
Braun
P E
PE
D000818:Animals; D006031:Glycosylation; D008562:Membrane Glycoproteins / Q000302:isolation & purification*; D008698:Mesylates; D051379:Mice; D009185:Myelin Proteins / Q000302:isolation & purification*; D018992:Myelin-Associated Glycoprotein; D010750:Phosphoproteins / Q000302:isolation & purification*
D008562:Membrane Glycoproteins; D008698:Mesylates; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D010750:Phosphoproteins; C012077:trifluoromethanesulfonic acid