[Obtaining of monoclonal antibodies to human growth hormone and their characteristics].
The aim of present study was to obtain the hybridomas producing monoclonal antibodies against human growth hormone (Mabs hGH), to investigate the properties of the obtained Mabs and the possibility of their application in immunoanalytical systems. Two hybridomas secreting Mabs against hGH and belonging to the IgGI subclass have been obtained. The cross-reactivity of the Mabs with structurally similar to hGH hormones (hGHbio, hPL, hPRL, bGH, bPRL, pPRL) using indirect IFA has been studied. It has been shown that Mabs hGHI and Mabs hGH2 are directed to common specific antigenic determinant i.e. they have the same epitope specificity and don't react with other structurally related hormones, i.-e. this determinant is unique for hGH. The obtained Mabs hGH2 would find application for determination hGH by immunochemical methods in fractions while the hormone isolation from pituitaries and hGH obtained recombinant DNA methodology. The development of immunosorbents on the basis Mabs hGH2 seems to be perspective. Application of this immunosorbent may give possibility to optimize hormone isolation process.
Osipova
T A
TA
Fidler
R
R
Grigor'ian
A L
AL
Papazov
I P
IP
Verbitskiĭ
M Sh
MSh
Bulatov
A A
AA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D003429:Cross Reactions; D000939:Epitopes; D013006:Growth Hormone / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D000939:Epitopes; D013006:Growth Hormone
Poluchenie monoklonal'nykh antitel k gormonu rosta cheloveka i ikh kharakteristika.