Characterization of the specificity of lipopolysaccharide antigens from seven Fisher's immunotypes of Pseudomonas aeruginosa, using ELISA technique.
Lipopolysaccharides of seven Fisher's immunotypes of P. aeruginosa, extracted by water-phenol method, were fractionated on Sepharose 2B column. On the basis of molecular weight sugar and KDO content, eluents were separated into 4 fractions. An analysis of the antigenic specificity of the chromatographic fractions of seven immunotypes of LPS was carried out, using sera of mice vaccinated with several crude LPS preparations or whole-cell suspensions each of P. aeruginosa immunotypes, by ELISA. The antigenic specificity of fraction 1 and 2 of several immunotypes (with the exception of LPS from immunotype 2) in reaction with mice antisera for crude LPS was shown. Not quite full specificity of fractions 1-3 of all LPS preparations during analysis of these fractions reactivity with antisera to whole P. aeruginosa cells were observed, but specific reactions predominated in all test systems except LPS 2.
Trafny
E A
EA
Department of Microbiology, Military Institute of Hygiene and Epidemiology, Warsaw, Poland.
Grzybowski
J
J
D000942:Antigens, Bacterial / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D008070:Lipopolysaccharides / Q000032:analysis / Q000276:immunology*; D008970:Molecular Weight; D010641:Phenotype; D011550:Pseudomonas aeruginosa / Q000276:immunology*
D000942:Antigens, Bacterial; D000939:Epitopes; D008070:Lipopolysaccharides