title: A new immunonephelometric method for amniotic fluid alpha-fetoprotein measurement.
abstract: We have evaluated the analytic performance of a new immunonephelometric method developed for the measurement of amniotic fluid alpha-fetoprotein (AF-AFP) and compared it with the classic immunoradiometric method used up to now. Intrassay precision, evaluated for three AF-AFP levels, was good and Interassay imprecision shows higher coefficients of variation (CVs) especially for medium and high AF-AFP levels. Linearity was demonstrated for level higher than 300,000 ng/ml and no hook effects was observed. Comparison with an IRMA method gave the following regression equation: y = 3050 + 0.52X (r = 0.76, p less than 0.001, n = 36).
authors: J L
authors: JL
authors: Bedini
authors: E
authors: E
authors: Casals
authors: C
authors: C
authors: Sanllehy
authors: X
authors: X
authors: Filella
authors: E
authors: E
authors: Mas
authors: R
authors: R
authors: Molina
authors: A M
authors: AM
authors: Ballesta
mesh_terms: D000653:Amniotic Fluid / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods; D009391:Nephelometry and Turbidimetry; D011247:Pregnancy; D013223:Statistics as Topic; D000509:alpha-Fetoproteins / Q000032:analysis*
chemical_list: D015415:Biomarkers; D000509:alpha-Fetoproteins