Affinity separation of proteins in aqueous three-phase systems.
Aqueous polymer three-phase systems composed of dextran-Ficoll-polyethylene glycol-water have been used for affinity partition of proteins. The upper, middle, and lower phases are rich in polyethylene glycol, Ficoll, and dextran, respectively. Affinity partition was performed using the reactive dyes Cibacron Blue F36-A and Remazol Yellow GCL which are known as specific ligands for albumin and prealbumin from human serum. When the ligands were bound alternatively to polyethylene glycol, Ficoll, or dextran the target proteins were directed toward the upper, middle, or lower phase, respectively. In the presence of two ligands immobilized to two different polymers the distribution of two proteins could be steered to different phases at the same time. Serum albumin and prealbumin could be separated by using Cibacron Blue-Ficoll and Remazol Yellow-dextran or Cibacron Blue-polyethylene glycol and Remazol Yellow-dextran as polymer ligands.
Albertsson
P A
PA
Department of Biochemistry, University of Lund, Sweden.
Birkenmeier
G
G
D000418:Albumins / Q000302:isolation & purification; D001391:Azo Compounds; D004396:Coloring Agents; D003911:Dextrans; D005362:Ficoll; D011092:Polyethylene Glycols; D011108:Polymers; D011228:Prealbumin / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification*; D014227:Triazines; D014867:Water
D000418:Albumins; D001391:Azo Compounds; D004396:Coloring Agents; D003911:Dextrans; D011108:Polymers; D011228:Prealbumin; D011506:Proteins; D014227:Triazines; D014867:Water; C035187:reactive yellow 13; D005362:Ficoll; D011092:Polyethylene Glycols; C012842:Cibacron Blue F 3GA