[Production and characterization of monoclonal antibodies against allergen components].
Monoclonal antibodies are useful tools for characterizing components causing type I allergy. Three aspects in production and characterization of monoclonal antibodies are discussed: 1. production of monoclonal antibodies raised against a special allergen of rye pollen extract. 2. investigation of monoclonal antibodies raised against peptide fragments of ovalbumin, 3. analysis of epitopes on allergenic components of timothy pollen extracts. The results indicate that monoclonal antibodies raised against certain allergens are useful for isolation of allergenic components and epitope mapping.
Petersen
A
A
Forschungsinstitut Borstel, Medizinsche Klinik, Laborgruppe Allergologie.
Kahlert
H
H
Jelke
H
H
Becker
W M
WM
Schlaak
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D006825:Hybridomas; D007525:Isoelectric Focusing; D051379:Mice; D010047:Ovalbumin / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology*; D006109:Poaceae; D011058:Pollen / Q000276:immunology*; D012434:Secale
D000911:Antibodies, Monoclonal; D000939:Epitopes; D010446:Peptide Fragments; D010047:Ovalbumin
Produktion und Charakterisierung von monoklonalen Antikörpern gegen allergene Komponenten.