Adenylyl cyclase amino acid sequence: possible channel- or transporter-like structure.
Complementary DNA's that encode an adenylyl cyclase were isolated from a bovine brain library. Most of the deduced amino acid sequence of 1134 residues is divisible into two alternating sets of hydrophobic and hydrophilic domains. Each of the two large hydrophobic domains appears to contain six transmembrane spans. Each of the two large hydrophilic domains contains a sequence that is homologous to a single cytoplasmic domain of several guanylyl cyclases; these sequences may represent nucleotide binding sites. An unexpected topographical resemblance between adenylyl cyclase and various plasma membrane channels and transporters was observed. This structural complexity suggests possible, unappreciated functions for this important enzyme.
Krupinski
J
J
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235.
Coussen
F
F
Bakalyar
H A
HA
Tang
W J
WJ
Feinstein
P G
PG
Orth
K
K
Slaughter
C
C
Reed
R R
RR
Gilman
A G
AG
D000262:Adenylyl Cyclases / Q000235:genetics / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology; D002352:Carrier Proteins; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D007473:Ion Channels; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011487:Protein Conformation; D014162:Transfection
D002352:Carrier Proteins; D007473:Ion Channels; D008565:Membrane Proteins; D004247:DNA; D000262:Adenylyl Cyclases