A human monoclonal autoantibody isolated from a patient with infectious mononucleosis reactive with both self antigens and Epstein-Barr virus nuclear antigen (EBNA).
In order to investigate the mechanism(s) by which Epstein-Barr virus (EBV) induces the outcome of autoantibodies during infectious mononucleosis (IM), a human IgM (k) monoclonal antibody to cytoskeletal filaments of epithelial cells has been prepared by EBV transformation of peripheral blood B lymphocytes obtained from a patient with IM. The antibody was also found to react with smooth muscle of frozen sections of human stomach tissue by immunofluorescence, and with the Epstein-Barr nuclear antigen (EBNA) by an enzyme-linked immunosorbent assay. These findings demonstrate at the clonal level the epitope homology between host's cell antigens and EBV-encoded nuclear antigen, which might have relevance in EBV-induced autoimmunity.
Garzelli
C
C
Department of Biomedicine, University of Pisa, Italy.
Pacciardi
A
A
Carmignani
M
M
Conaldi
G
G
Basolo
F
F
Falcone
G
G
D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification*; D000956:Antigens, Viral / Q000276:immunology*; D001323:Autoantibodies / Q000276:immunology*; D001324:Autoantigens; D001402:B-Lymphocytes / Q000276:immunology; D002472:Cell Transformation, Viral; D003599:Cytoskeleton / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D004848:Epithelium / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D019309:Epstein-Barr Virus Nuclear Antigens; D005455:Fluorescent Antibody Technique; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D007244:Infectious Mononucleosis / Q000276:immunology*; D009130:Muscle, Smooth / Q000276:immunology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D001323:Autoantibodies; D001324:Autoantigens; D000939:Epitopes; D019309:Epstein-Barr Virus Nuclear Antigens