Presence of a substance P-like immunoreactive neurone system from the parabrachial area to the central amygdaloid nucleus of the rat with reference to coexistence with calcitonin gene-related peptide.
An ascending neurone system containing substance P-like immunoreactivity (SPI) from the lateral parabrachial nucleus (PBL) to the central amygdaloid nucleus (AC) was detected. Destruction of the external subdivision of the PBL resulted in a marked ipsilateral reduction of SPI fibres in the AC, which suggests that SPI neurones project mainly ipsilaterally to the AC. This was supported by the findings that injection of biotin-wheatgerm agglutinin into the AC labelled many neurones in the ipsilateral external subdivision of the PBL. Simultaneous staining with antiserum showed that some of these neurones contain SP. Immunohistochemical double-staining revealed that almost all of the SPI neurones in the external subdivision of the PBL contained calcitonin gene-related peptide.
Yamano
M
M
Department of Anatomy II, Osaka University Medical School, Japan.
Hillyard
C J
CJ
Girgis
S
S
MacIntyre
I
I
Emson
P C
PC
Tohyama
M
M
D000679:Amygdala / Q000166:cytology*; D000818:Animals; D015740:Calcitonin Gene-Related Peptide; D007150:Immunohistochemistry; D009434:Neural Pathways / Q000166:cytology; D009474:Neurons / Q000032:analysis; D009479:Neuropeptides / Q000032:analysis*; D051381:Rats; D013373:Substance P / Q000032:analysis*
D009479:Neuropeptides; D013373:Substance P; D015740:Calcitonin Gene-Related Peptide