10-N nonyl-acridine orange: a fluorescent probe which stains mitochondria independently of their energetic state.
The specificity of binding of 10-N Nonyl Acridine Orange to mitochondria, and more precisely to inner membranes, is demonstrated by subcellular fractionation of hepatocytes. Unlike Rhodamine 123, which is a preferential marker of the transmembrane potential, Nonyl Acridine Orange binding is essentially independent of the mitochondria energization state although a low uptake of this dye, in response to the potential, may be measured. So 10-N Nonyl acridine orange is an appropriate marker of the mitochondial membrane surface per unit of cell mass.
Maftah
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Genius Unité de Biotechnologie, Faculté des sciences, Limoges, France.
Petit
J M
JM
Ratinaud
M H
MH
Julien
R
R
D000165:Acridine Orange / Q000031:analogs & derivatives*; D000818:Animals; D003576:Electron Transport Complex IV / Q000378:metabolism; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D008099:Liver / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D051379:Mice; D008930:Mitochondria, Liver / Q000201:enzymology / Q000378:metabolism*; D051381:Rats; D012235:Rhodamines / Q000493:pharmacokinetics; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling
D005456:Fluorescent Dyes; D012235:Rhodamines; C056258:N(10)-nonylacridine orange; D003576:Electron Transport Complex IV; D000165:Acridine Orange