Adsorption of vitronectin in human serum onto plastics is augmented by sodium dodecyl sulfate.
We have investigated the adsorption of cell-spreading activity in human serum onto polystyrene plates after treatment of the serum with sodium dodecyl sulfate (SDS). Vitronectin in human serum was remarkably adsorbed onto the plate after boiling the serum with 0.1% SDS for 5 min. SDS was effective over the concentration range from 0.05 to 0.25%. Increase of the vitronectin adsorption was accompanied by an increase of cell spreading on the plates. The cell-spreading activity in SDS-treated serum was impeded by anti-vitronectin antibody but not by anti-fibronectin antibody. After treatment with SDS, fibronectin-depleted serum could induce cell spreading but vitronectin-depleted serum could not. These results indicate that vitronectin alone was the cell-spreading factor in SDS-treated human serum. However, SDS-treated pure vitronectin itself did not retain the cell-spreading activity. The activity was recovered when bovine serum albumin was added to pure vitronectin before or after boiling with 0.1% SDS. Therefore, vitronectin adsorbed from SDS-treated serum might retain the cell-spreading activity with the aid of serum protein. Treatment of serum with SDS provides an easy, specific, and efficient method of coating polystyrene plates with vitronectin.
Miyamoto
Y
Y
Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan.
Izumi
M
M
Ishizaka
S
S
Hayashi
M
M
D000327:Adsorption; D002448:Cell Adhesion; D002465:Cell Movement; D002478:Cells, Cultured; D005353:Fibronectins / Q000097:blood; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D066298:In Vitro Techniques; D011137:Polystyrenes; D012967:Sodium Dodecyl Sulfate; D019096:Vitronectin
D005353:Fibronectins; D006023:Glycoproteins; D011137:Polystyrenes; D019096:Vitronectin; D012967:Sodium Dodecyl Sulfate