title: [Increased expression of IGF-2 in tumor tissue of nephroblastoma].
abstract: The incidence of nephroblastoma is increased in a number of syndromes with abnormal growth pattern. Elevated IGF 2-expression has been documented in various Wilms' tumors and a defect in the IGF 2 gene has been noted in 1 case. Southern blot analysis of genomic DNA of nephroblastomas in 5 additional patients after restriction enzyme digest with EcoRI, Pvu II, Pst I and Taq I did not reveal any defect in the IGF 2 gene. Slot blot analysis however showed marked overexpression of IGF 2-mRNA reaching up to more than 25 times the level expressed in unaffected kidney tissue adjacent to the tumor and in normal kidney tissue used as controls.
authors: J
authors: J
authors: Briner
authors: S
authors: S
authors: Hassam
authors: E J
authors: EJ
authors: Schönle
authors: U
authors: U
authors: Rohrer
mesh_terms: D015139:Blotting, Southern; D002880:Chromosomes, Human, Pair 11; D004273:DNA, Neoplasm / Q000235:genetics; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D007680:Kidney Neoplasms / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D013002:Somatomedins / Q000235:genetics*; D009396:Wilms Tumor / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm; D013002:Somatomedins; D007335:Insulin-Like Growth Factor II
vernacular_title: Nachweis erhöhter Expression von IGF 2 im Tumorgewebe von Nephroblastomen.