Tetrodotoxin-sensitive voltage-dependent Na currents recorded from Xenopus oocytes injected with mammalian cardiac muscle RNA.
Voltage-sensitive sodium (Na) channel currents recorded from mammalian cardiac muscle are blocked by tetrodotoxin (TTX) with a Kd of 1-3 microM. We have observed a Kd for TTX of 4-10 nM for Na currents recorded from Xenopus oocytes injected with RNA extracted from rabbit cardiac muscle. This result suggests that the degree of TTX sensitivity of Na channels encoded by cardiac muscle mRNA is in part determined by post-translational modification(s) or of associations with accessory proteins in the membrane.
Sutton
F
F
Division of Biology, California Institute of Technology, Pasadena 91125.
Davidson
N
N
Lester
H A
HA
D000818:Animals; D005260:Female; D006321:Heart / Q000502:physiology*; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics*; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics*; D012964:Sodium / Q000378:metabolism*; D015222:Sodium Channels; D013779:Tetrodotoxin / Q000494:pharmacology*; D014981:Xenopus
D007473:Ion Channels; D008565:Membrane Proteins; D012333:RNA, Messenger; D015222:Sodium Channels; D013779:Tetrodotoxin; D012964:Sodium