title: Monoclonal antibody Ki-67 defined growth fraction in benign prostatic hyperplasia and prostatic cancer.
abstract: Growth fractions were assessed immunohistochemically in prostatic tissues with benign glandular hyperplasia (BPH) and in specimens of prostatic cancer using the monoclonal antibody Ki-67. This antibody is specific for a proliferation-associated nuclear antigen. In BPH tissues about 0.3% of nuclei of epithelial cells was reactive with Ki-67. The Ki-67 positive nuclei were distributed equally among the basal and luminal cells of the hyperplastic prostatic acini. In prostatic cancer the Ki-67 defined growth fraction ranged from 0.4% to 9.1% (mean value 2.9%). Cancers with a cribriform growth pattern and tumors composed of solid areas of undifferentiated cancer cells showed the highest growth fraction (average values 4.0%, respectively 7.6%). The investigated four tumors composed of undifferentiated solitary tumor cells with diffuse infiltration of the stroma demonstrated an unexpectedly low growth activity (average 1.2%). In cancers with a glandular growth pattern the Ki-67 defined growth fraction of tumor cells varied from 2.2% to 5%. Compared with other epithelial tumors these values are low, but they are in agreement with the earlier findings on prostatic cancer obtained with 3H-thymidine labeling and bromodeoxyuridine incorporation. The observed variation in the level of Ki-67 defined growth activity partly related to the histological tumor pattern suggests that Ki-67 labeling may serve as a prognostic factor additional to the current histopathological grading criteria of prostatic cancer.
authors: Department of Pathology, Erasmus University Rotterdam, The Netherlands.
authors: M P
authors: MP
authors: Gallee
authors: E
authors: E
authors: Visser-de Jong
authors: F J
authors: FJ
authors: ten Kate
authors: F H
authors: FH
authors: Schroeder
authors: T H
authors: TH
authors: Van der Kwast
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology / Q000473:pathology; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000276:immunology; D002277:Carcinoma / Q000276:immunology / Q000473:pathology; D002455:Cell Division; D002467:Cell Nucleus / Q000276:immunology / Q000648:ultrastructure; D006801:Humans; D008297:Male; D008938:Mitosis; D009687:Nuclear Proteins / Q000276:immunology; D011470:Prostatic Hyperplasia / Q000276:immunology / Q000473:pathology*; D011471:Prostatic Neoplasms / Q000276:immunology / Q000473:pathology*
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D009687:Nuclear Proteins