title: Induction of histamine release from rat mast cells and human basophilic granulocytes by clinical Escherichia coli isolates and relation to hemolysin production and adhesin expression.
abstract: We investigated the role of 27 disease-relevant Escherichia coli strains isolated from humans in the induction of histamine release from rat peritoneal mast cells and human basophilic granulocytes. Our data indicated that only the hemolysin-positive (HLY+) bacteria and the hemolysin-positive culture supernatants induced histamine release. For the latter, the hemolysin activity determined the degree of histamine secretion. Incubation of the target cells with washed HLY+ bacteria revealed a different secretory response. For the rat mast cells, histamine release paralleled expression of hemolysin activity, with the exception of strain S98 (O75:K5:H- HLY+), which induced less histamine, although its hemolysin activity was relatively high. No correlation between histamine secretion and hemolysin activity was observed when human basophils were stimulated with the HLY+ bacteria. Large amounts of histamine were still released, even when the hemolysin activity declined to zero. Our results support the potent role of the E. coli hemolysin as a pathogenicity factor in bacterial infection.
authors: Lehrstuhl für Medizinische Mikrobiologie und Immunologie, Ruhr-Universität Bochum, Federal Republic of Germany.
authors: W
authors: W
authors: Gross-Weege
authors: W
authors: W
authors: König
authors: J
authors: J
authors: Scheffer
authors: W
authors: W
authors: Nimmich
mesh_terms: D018830:Adhesins, Escherichia coli; D000818:Animals; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis; D001491:Basophils / Q000378:metabolism*; D002478:Cells, Cultured; D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000378:metabolism / Q000502:physiology*; D006384:Hemagglutination; D006460:Hemolysin Proteins / Q000096:biosynthesis*; D006636:Histamine Release; D006801:Humans; D007700:Kinetics; D008407:Mast Cells / Q000378:metabolism*; D051381:Rats
chemical_list: D018830:Adhesins, Escherichia coli; D001425:Bacterial Outer Membrane Proteins; D006460:Hemolysin Proteins