cDNA cloning of mouse myelin-associated glycoprotein: a novel alternative splicing pattern.
The structures of three forms of mouse myelin-associated glycoprotein mRNAs were determined from full-length cDNA clones. Two forms of mRNAs have been reported to be different by alternate inclusion of exon 2 and 12 in rat brain. One of the three forms of clones obtained here appeared to be a novel mRNA which lacked both the exon 2 and 12 portions, although others were identical splicing patterns to those of rat. Northern blot analysis using specific probes to mRNAs with or without the exon 2 portion in normal and quaking mouse confirmed that the splicing of exon 2 and 12 occurred independently.
Fujita
N
N
Department of Neurology, Niigata University, Japan.
Sato
S
S
Kurihara
T
T
Kuwano
R
R
Sakimura
K
K
Inuzuka
T
T
Takahashi
Y
Y
Miyatake
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D005091:Exons; D051379:Mice; D008821:Mice, Quaking; D008969:Molecular Sequence Data; D009185:Myelin Proteins / Q000235:genetics*; D018992:Myelin-Associated Glycoprotein; D009693:Nucleic Acid Hybridization; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D015183:Restriction Mapping
D015342:DNA Probes; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D012333:RNA, Messenger; D004247:DNA