T cell lines selected with synthetic peptides are highly encephalitogenic in SJL/J mice.
T cell lines were selected from basic protein (BP)-immunized SJL/J mice using synthetic peptides encompassing the major SJL/J encephalitogenic determinant. Synthetic peptide-derived T cell lines proliferated in response to BP, the 89-169 peptidase fragment of BP and the synthetic peptides, pM87-99, pM90-99 and pM91-99. These lines transferred a demyelinating and chronic relapsing form of experimental autoimmune encephalomyelitis (EAE) into naive mice, and EAE induced by synthetic peptide-derived lines was more severe than that induced by whole BP-derived lines. This study demonstrates that T cell lines selected with synthetic peptides are encephalitogenic in SJL/J mice and offers an improved means for selecting SJL/J encephalitogenic T cell lines.
Bourdette
D N
DN
Neuroimmunology Research, VA Medical Center, Portland, Oregon 97201.
Vandenbark
A A
AA
Hashim
G
G
Whitham
R H
RH
Offner
H
H
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D004660:Encephalitis / Q000276:immunology* / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D004676:Myelin Basic Protein / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000502:physiology
D004676:Myelin Basic Protein; D010446:Peptide Fragments; D010455:Peptides