Alpha-fetoprotein-producing capacity, chromosomal and morphological properties in human hepatoma cells treated with various chemotherapeutic agents.
Alpha-fetoprotein (AFP)-producing capacity and some other properties of human hepatoma cell lines treated with chemotherapeutic agents, such as mitomycin C, Adriamycin, cisplatinum, and 5-fluorouracil were investigated. In the case of hepatoma cells that can be grown in culture following treatment with chemotherapeutic agents, their AFP-producing capacity was almost equal to that of untreated control cells with a few exceptions. On the other hand, in the case of similarly treated hepatoma cells that cannot be grown in culture, their AFP-producing capacity was quite different from that of untreated control cells. Under these conditions chromosomal and morphological aberrations were also observed in the treated cells. The present study shows that AFP-producing capacity of hepatoma cells can be changed by chemotherapeutic agents, probably through chromosomal mutation.
Muraoka
A
A
Division of Pathology, Okayama University Medical School, Japan.
Tokiwa
T
T
Sato
J
J
D000970:Antineoplastic Agents / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D002455:Cell Division / Q000187:drug effects; D002869:Chromosome Aberrations; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D008858:Microscopy, Phase-Contrast; D014407:Tumor Cells, Cultured / Q000187:drug effects; D000509:alpha-Fetoproteins / Q000096:biosynthesis*
D000970:Antineoplastic Agents; D000509:alpha-Fetoproteins