Demonstration of immunochemical identity between the synaptic vesicle proteins synaptin and synaptophysin/p38.
The synaptic vesicle proteins synaptin and synaptophysin/p38 were shown to be immunochemically identical. Western immunoblot analysis of Triton X-100 extracts from rat brain showed that polyclonal polyspecific anti-synaptin antibodies and monoclonal antibody SY38 against synaptophysin both reacted with a band of 38 kDa. In two-dimensional immunoblots of chromaffin granule membranes from bovine adrenal medulla anti-synaptin and anti-synaptophysin antibodies also recognized the same component. Finally, in a Western immunoblotting experiment SY38 reacted with an immuno-isolated synaptin antigen.
Gaardsvoll
H
H
Research Center for Medical Biotechnology, University of Copenhagen, Denmark.
Obendorf
D
D
Winkler
H
H
Bock
E
E
D000313:Adrenal Medulla / Q000032:analysis; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D015153:Blotting, Western; D001923:Brain Chemistry; D002837:Chromaffin Granules / Q000032:analysis; D000939:Epitopes / Q000276:immunology*; D007163:Immunosorbent Techniques; D008565:Membrane Proteins / Q000032:analysis / Q000276:immunology*; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000276:immunology*; D051381:Rats; D013572:Synaptic Vesicles / Q000032:analysis*; D016708:Synaptophysin
D000911:Antibodies, Monoclonal; D000941:Antigens; D000939:Epitopes; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D016708:Synaptophysin; C030625:synaptin