Involvement of endopeptidase-24.11 in degradation of substance P by glioma cells.
C6 of rat glioma cells and their plasma membranes degrade substance P (SP). The degradation, occurring mainly through the cleavage of the Gln6-Phe7, Phe7-Phe8, and Gly9-Leu10 bonds, was strongly inhibited by phosphoramidon. Endopeptidase-24.11 (EC 3.4.24.11) purified from C6 cell membranes also cleaved SP at the same three peptide bonds in a manner sensitive to phosphoramidon. Thus, the degradation of SP by glioma cells and their membranes seems to be mediated by the action of endopeptidase-24.11.
Endo
S
S
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Yokosawa
H
H
Ishii
S
S
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism; D005910:Glioma / Q000378:metabolism; D006868:Hydrolysis; D015260:Neprilysin / Q000302:isolation & purification / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism; D013373:Substance P / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000378:metabolism*
D000596:Amino Acids; D010446:Peptide Fragments; D013373:Substance P; D015260:Neprilysin