title: Circulating human blood platelets retain appreciable amounts of poly (A)+ RNA.
abstract: Platelets lack a nucleus and are usually considered to be incapable of protein synthesis due to an apparent lack of messenger RNA, precluding the construction of platelet cDNA libraries and hindering the cloning of authentic platelet cDNA's. We reasoned that vestigial amounts of messenger RNA may remain in platelets when they first separate from the megakaryocyte and circulate in the peripheral blood. We isolated poly (A)+ RNA from platelets obtained by pheresis of individuals with elevated blood platelet counts due to a myeloproliferative syndrome termed essential thrombocythemia. Northern blots using probes for platelet glycoprotein Ib indicate that the poly (A)+ RNA obtained from the platelets of these donors is, in fact, derived from platelets. Cell free translation studies using the platelet poly (A)+ RNA indicate that the material is translationally active. We conclude that, contrary to prevailing information, circulating human blood platelets retain appreciable amounts of poly (A)+ RNA and that this RNA can be harvested by the described approach. The poly (A)+ RNA provides templates for the synthesis of cDNA's that code for platelet proteins.
authors: Hematology Section, Veterans Administration Medical Center, Seattle, WA 98108.
authors: G J
authors: GJ
authors: Roth
authors: M J
authors: MJ
authors: Hickey
authors: D W
authors: DW
authors: Chung
authors: D D
authors: DD
authors: Hickstein
mesh_terms: D001792:Blood Platelets / Q000378:metabolism*; D015152:Blotting, Northern; D006801:Humans; D009504:Neutrophils / Q000032:analysis; D010983:Plateletpheresis; D011061:Poly A / Q000097:blood* / Q000302:isolation & purification; D012313:RNA / Q000097:blood* / Q000302:isolation & purification; D013922:Thrombocytosis / Q000097:blood / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: D011061:Poly A; D012313:RNA