title: Neurotoxic effects of p-chloroamphetamine on the serotoninergic innervation of the trigeminal motor nucleus: a retrograde transport study.
abstract: The rat forebrain receives projections from both dorsal and median raphe nuclei. It has recently been shown that serotoninergic axons arising from the dorsal raphe nucleus, but not those from the median raphe nucleus, degenerate following systemic administration of p-chloroamphetamine (PCA). The present study was conducted to determine (i) whether the motor nucleus of the trigeminal nerve is innervated by overlapping projections from multiple serotonin cell groups and (ii) whether a particular subset of serotoninergic axon terminals in the trigeminal motor nucleus are sensitive to the neurotoxic effects of PCA. Retrograde transport was used in combination with immunofluorescence to identify the serotonin-positive cells that project to the trigeminal motor nucleus both in control rats and in rats previously treated with PCA. In untreated rats, an average of 95 retrogradely labeled serotonin-positive neurons were found in the dorsal raphe nucleus, 135 in the nucleus raphe obscurus, 132 in the nucleus raphe pallidus and 63 in the ventrolateral medulla. After treatment with PCA, there was a marked decrease (-77%) in the number of retrogradely labeled serotoninergic neurons in the dorsal raphe nucleus, whereas the number of labeled neurons was unchanged in the raphe obscurus and raphe pallidus. These results demonstrate that PCA selectively lesions serotonin axon terminals arising from the dorsal raphe nucleus, while sparing projections from the raphe obscurus and raphe pallidus to the trigeminal motor nucleus. This conclusion is in agreement with previous findings that in the forebrain only axons from the dorsal raphe are vulnerable to PCA. The data provide further evidence that serotoninergic axons originating in the dorsal raphe nucleus differ from other serotoninergic axons in their pharmacological properties and that the dorsal raphe may contain a functionally unique subset of serotonin neurons.
authors: Johns Hopkins University School of Medicine, Department of Neuroscience, Baltimore, MD 21205.
authors: J M
authors: JM
authors: Fritschy
authors: W E
authors: WE
authors: Lyons
authors: M E
authors: ME
authors: Molliver
authors: R
authors: R
authors: Grzanna
mesh_terms: D000662:Amphetamines / Q000494:pharmacology*; D000818:Animals; D001370:Axonal Transport / Q000187:drug effects; D008297:Male; D009474:Neurons / Q000166:cytology / Q000502:physiology; D009498:Neurotoxins; D011903:Raphe Nuclei / Q000187:drug effects / Q000502:physiology; D051381:Rats; D011919:Rats, Inbred Strains; D012016:Reference Values; D012701:Serotonin / Q000502:physiology*; D014278:Trigeminal Nuclei / Q000187:drug effects / Q000502:physiology*; D010133:p-Chloroamphetamine / Q000494:pharmacology*
chemical_list: D000662:Amphetamines; D009498:Neurotoxins; D012701:Serotonin; D010133:p-Chloroamphetamine