Melanin-related metabolites in urine of B16 melanoma-bearing mice.
5-S-Cysteinyldopa (5-S-CD), a pheomelanin precursor, has been used as a biochemical marker of melanoma metastasis. Recently, 5-hydroxy-6-methoxyindole-2-carboxylic acid (5H6MI2C), a eumelanin-related metabolite, was shown to reflect well the degree of skin pigmentation. We measured the urinary excretion of 5H6MI2C and 5-S-CD in mice bearing B16 melanoma to determine which of the two markers better reflects the progression of melanoma. The urinary excretion of both 5H6MI2C and 5-S-CD increased rapidly in parallel with the tumour volume. The highest values for the two metabolites in melanoma-bearing mice were three orders of magnitude higher than those in control mice. However, 5H6MI2C had a higher excretion level at the early stage of melanoma progression, while 5-S-CD had a higher excretion level at the later stage.
Wakamatsu
K
K
School of Hygiene, Fujita-Gakuen Health University, Japan.
Ito
S
S
Fujita
K
K
D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D003548:Cysteinyldopa / Q000652:urine*; D004295:Dihydroxyphenylalanine / Q000031:analogs & derivatives*; D004298:Dopamine / Q000652:urine; D007211:Indoles / Q000652:urine*; D008543:Melanins / Q000378:metabolism*; D008546:Melanoma, Experimental / Q000175:diagnosis / Q000473:pathology / Q000652:urine*; D051379:Mice; D008810:Mice, Inbred C57BL
D014408:Biomarkers, Tumor; D007211:Indoles; D008543:Melanins; D003548:Cysteinyldopa; C017407:5-hydroxy-6-methoxy-2-indolylcarboxylic acid; C034513:2-S-cysteinyldopa; C017408:6-hydroxy-5-methoxy-2-indolylcarboxylic acid; D004295:Dihydroxyphenylalanine; D004298:Dopamine