[Comparison of various staining methods for boar's sperm].
303 semen smear from the ejaculates of 101 boars were prepared with 3 different staining techniques. The staining methods were: the Cerovsky, the Farelly, the Wells-Awa. Native semen smear were examined by phase contrast microscope. The examined sperm abnormalities were the following: acrosomal anomalies, cytoplasmatic droplets (proximal and distal), tail anomalies (hairpin curved, single bent, coiled tails) and head anomalies. The phase contrast microscope technique have not found useful for detecting of acrosome and head anomalies. The method we have found most useful for staining the acrosome was the Wells-Awa, but this staining technique was not suitable for detecting of cytoplasmatic droplets. Farelly and Cerovsky staining technique have appeared highly desirable for staining of sperm abnormalities. The Cerovsky staining technique was proved most useful in everyday veterinary practice, as it needs very short time and shows the same rate in detecting acrosome anomalies than the Wells-Awa staining technique.
Wekerle
L
L
Forschungszentrum für Tierproduktion und Tierernährung Gödöllö - Herceghalom/Hungary.
Sarlós
P
P
D000177:Acrosome / Q000032:analysis; D000818:Animals; D007248:Infertility, Male / Q000473:pathology / Q000662:veterinary; D008297:Male; D008858:Microscopy, Phase-Contrast; D013077:Sperm Head / Q000473:pathology; D013094:Spermatozoa / Q000002:abnormalities / Q000032:analysis*; D013194:Staining and Labeling; D013552:Swine / Q000502:physiology*; D013553:Swine Diseases / Q000473:pathology
Vergleich verschiedener Färbemethoden von Eberspermien.