A stage-specific calcium-binding protein from microfilariae of Brugia malayi (Filariidae).
The monoclonal antibody MF1 has been shown to cause transient clearance of blood-borne microfilariae in gerbils infected with Brugia malayi. The present study demonstrates that the MF1 antibody recognizes a proteinaceous determinant on two polypeptides of 70 and 75 kDa. Both the monoclonal antibody and antisera raised to the gel-purified antigen show binding restricted to mature microfilariae. Intrauterine and recently shed microfilariae apparently lack the MF1 molecules. Prominent 45Ca-binding molecules comigrate with the MF1 antigens in one-dimensional electrophoresis (under both reducing and non-reducing conditions), and co-purify through ion exchange chromatography. The MF1 antigen thus appears to be a developmentally regulated calcium-binding protein(s).
Fuhrman
J A
JA
Department of Tropical Public Health, Harvard School of Public Health, Boston, MA 02115.
Piessens
W F
WF
D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis* / Q000302:isolation & purification; D015153:Blotting, Western; D002009:Brugia / Q000032:analysis* / Q000254:growth & development / Q000276:immunology; D002135:Calcium-Binding Proteins / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D002852:Chromatography, Ion Exchange; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005260:Female
D000947:Antigens, Helminth; D002135:Calcium-Binding Proteins; D000939:Epitopes