Immortalization of rat keratinocytes by transfection with polyomavirus large T gene.
Transfection of primary rat keratinocytes with the polyomavirus large T gene promotes the establishment of cell lines. The keratinocytes express the large T protein and can be continuously cultured in medium containing a low concentration of calcium. The immortalized keratinocytes retain the ability to differentiate when the calcium concentration is increased to normal levels.
Bayley
S A
SA
Biosciences Division, Unilever Research, Sharnbrook, Bedford, United Kingdom.
Stones
A J
AJ
Smith
C G
CG
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D002118:Calcium / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D000078404:Epidermal Cells; D005455:Fluorescent Antibody Technique; D007633:Keratins / Q000378:metabolism*; D008854:Microscopy, Electron; D010957:Plasmids; D051381:Rats; D014162:Transfection
D000952:Antigens, Polyomavirus Transforming; D007633:Keratins; D002118:Calcium