The effect of serine esterase inhibitors on ionophore-induced histamine release from human pulmonary mast cells.
The serine proteases tryptase and chymase are present in human pulmonary mast cells. About 10-100 times more tryptase than chymase is found in these cells. However, a clear physiological role for both enzymes remains to be elucidated; angiotensin processing has been proposed as one possible function of chymase. A dose-dependent inhibition of A23187-induced histamine release from dispersed human lung mast cells was observed after pretreatment with the serine protease inhibitor diisopropylfluorophosphate (DFP) or the chymotrypsin-like enzyme inhibitor N-tosyl-L-phenylalanine chloromethylketone (TPCK) but not with the trypsin-like enzyme inhibitor N-tosyl-L-lysine chloromethylketone (TLCK). These results indicate that a chymase is probably an important factor in a late phase of human lung mast cell activation.
Hultsch
T
T
Department of Haematology, Oncology and Immunology, University of Marburg, FRG.
Ennis
M
M
Heidtmann
H H
HH
D000001:Calcimycin / Q000494:pharmacology; D004950:Esterases / Q000037:antagonists & inhibitors*; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D007531:Isoflurophate / Q000494:pharmacology; D008168:Lung / Q000187:drug effects / Q000276:immunology; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology; D014107:Tosyllysine Chloromethyl Ketone / Q000494:pharmacology; D014108:Tosylphenylalanyl Chloromethyl Ketone / Q000494:pharmacology
D007531:Isoflurophate; D014107:Tosyllysine Chloromethyl Ketone; D000001:Calcimycin; D014108:Tosylphenylalanyl Chloromethyl Ketone; D004950:Esterases; C022635:serine esterase