Inhibition of in vitro fertilization by a monoclonal antibody reacting with the zona pellucida of the oviductal egg but not with that of the ovarian egg of the golden hamster.
A glycoprotein designated ZP-0 is secreted from the epithelial cells of the oviduct and is bound to the zonae pellucidae (ZPe) of the oviductal eggs. We have previously established a monoclonal antibody reacting with ZP-0, termed AZPO-8. In the present study, we have examined the effect of AZPO-8 on in vitro fertilization in the hamster. Treatment of the oviductal eggs with AZPO-8 at concentrations of more than 100 micrograms/ml resulted in: (1) the formation of "zona precipitation" on the outer region of the ZP; (2) inhibition of sperm binding to ZP; (3) the acquisition of resistance to protease treatment. Fertilization was inhibited by AZPO-8 in a dose-dependent manner. These results indicate that a monoclonal antibody (MoAb) to an additional component of ZP derived from the oviduct shows an inhibitory effect on fertilization in vitro.
Sakai
Y
Y
Department of Parasitology, Yamagata University School of Medicine, Japan.
Araki
Y
Y
Yamashita
T
T
Kurata
S
S
Oikawa
T
T
Hiroi
M
M
Sendo
F
F
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D006224:Cricetinae; D000939:Epitopes / Q000276:immunology; D005260:Female; D005307:Fertilization in Vitro; D008297:Male; D008647:Mesocricetus; D010053:Ovary; D010057:Oviducts; D010063:Ovum / Q000276:immunology*; D011247:Pregnancy; D013084:Sperm-Ovum Interactions; D015044:Zona Pellucida / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes