[The production of spread synaptonemal complexes of meiocytes from animals and plants by a drying method].
A spreading technique was used to prepare synaptonemal complexes from animals (Bombyx mori and Mus musculus spermatocytes) and plants (Zea mays microsporocytes). Suspension of hypotonically treated cells was spread over plastic-coated slides and air-dried. Fixed and phosphotungsten acid-stained spread preparations were studied by phase-contrast and electron microscopes. The spreading-through-drying method is more efficient and thereby more preferable as compared to the classical hypophase spreading technique.
Burd
S B
SB
Belousova
G G
GG
Klimenko
V V
VV
D000818:Animals; D012831:Bombyx; D002458:Cell Fractionation; D003890:Desiccation / Q000379:methods*; D008297:Male; D008540:Meiosis; D051379:Mice; D008854:Microscopy, Electron / Q000379:methods; D008858:Microscopy, Phase-Contrast / Q000379:methods; D013090:Spermatocytes / Q000648:ultrastructure; D013194:Staining and Labeling / Q000379:methods; D013573:Synaptonemal Complex; D003313:Zea mays
Poluchenie rasplastannykh sinaptonemnykh kompleksov meĭotsitov zhivotnykh i rasteniĭ metodom vysushivaniia.