Net efflux of chloride from cell suspensions measured with a K+ electrode.
Under appropriate conditions (presence of cation ionophores) net KCl efflux measured with a K+ electrode can be used to estimate conductive Cl- fluxes, a sensitive procedure that allows continuous recording. The procedure was tested in human red cells by demonstrating effects of ionophores and of an anion transport inhibitor, and in dissociated MDCK cells by demonstration of cAMP and volume-activated Cl- fluxes.
Rothstein
A
A
Research Institute, Hospital for Sick Children, Toronto, Canada.
Mack
E
E
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D001692:Biological Transport; D002712:Chlorides / Q000378:metabolism*; D005576:Colforsin / Q000494:pharmacology; D004566:Electrodes; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006096:Gramicidin / Q000494:pharmacology; D006801:Humans; D011188:Potassium; D014634:Valinomycin / Q000494:pharmacology
D002712:Chlorides; D006096:Gramicidin; D005576:Colforsin; D014634:Valinomycin; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D011188:Potassium