Photodynamic toxicity of hematoporphyrin derivatives to human keratinocytes in culture.
Human keratinocytes in culture were able to take up hematoporphyrin derivatives (HPDs) used during photodynamic chemotherapy of tumors. In the absence of light, HPDs showed no cytotoxic effects to keratinocytes. However, after irradiation with visible light, HPDs induced immediate cytotoxicity as measured by the neutral red uptake assay. On the other hand, cell attachment as measured by protein estimation was not affected. When the cells treated with HPDs and irradiated with light were cultured for a further 72 h, they partially lost their ability to attach to the collagen surface. Most of the cells remaining attached after 72 h were no longer viable following treatment with HPDs and light. All parameters measured depended on the intracellular concentration of HPDs used (7-50 ng/10(5) cells) and the time of irradiation (0-30 min). These results suggest that human keratinocytes are a good model to study cytotoxic effects of photodynamically active drugs. Further, keratinocytes were unable to recover after damage caused by HPDs and light.
Kappus
H
H
Department of Dermatology, Rudolf Virchow Clinic, Free University of Berlin, Federal Republic of Germany.
Reinhold
C
C
Artuc
M
M
D002478:Cells, Cultured; D004817:Epidermis / Q000187:drug effects*; D017324:Hematoporphyrin Derivative; D006415:Hematoporphyrins / Q000633:toxicity*; D006801:Humans; D007633:Keratins; D011838:Radiation-Sensitizing Agents / Q000633:toxicity*
D006415:Hematoporphyrins; D011838:Radiation-Sensitizing Agents; D007633:Keratins; D017324:Hematoporphyrin Derivative