title: 3'-Fluoro-3'-deoxyribonucleoside 5'-triphosphates: synthesis and use as terminators of RNA biosynthesis.
abstract: 3'-Fluoro-3'-deoxy-uridine, -cytidine, -adenosine and -guanosine have been synthesized by glycosylation of the corresponding silylated bases with 1-O-acetyl-2,5-di-O-benzoyl-3-fluoro-3-deoxy-D-ribofuranose in the presence of Friedel-Crafts catalysts and were converted to the 5'-triphosphates, NTP(3'-F). It was shown that NTP(3'-F) are terminators of RNA synthesis catalyzed by DNA-dependent RNA polymerase from E. coli and may thus serve as tools for DNA sequencing.
authors: Institute of Bioorganic Chemistry, Byelorussian SSR Academy of Sciences, Minsk.
authors: I A
authors: IA
authors: Mikhailopulo
authors: T I
authors: TI
authors: Pricota
authors: N E
authors: NE
authors: Poopeiko
authors: G G
authors: GG
authors: Sivets
authors: E I
authors: EI
authors: Kvasyuk
authors: T V
authors: TV
authors: Sviryaeva
authors: L P
authors: LP
authors: Savochkina
authors: R S
authors: RS
authors: Beabealashvilli
mesh_terms: D001483:Base Sequence; D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D003854:Deoxyribonucleotides / Q000138:chemical synthesis* / Q000494:pharmacology; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000201:enzymology; D008969:Molecular Sequence Data; D012313:RNA / Q000037:antagonists & inhibitors / Q000096:biosynthesis*
chemical_list: D003854:Deoxyribonucleotides; D012313:RNA; D004259:DNA-Directed DNA Polymerase