Monoclonal antibody against a peptide specified by [Met]-enkephalin complementary RNA recognizes the delta-class opioid receptor.
Monoclonal antibodies (MAB) were developed which recognize a peptide, His-Glu-Ala-Pro-Ile (HEAPI), encoded by the RNA complementary to the mRNA specifying [Met]-enkephalin. One such MAB (designated 6193) exhibited a high degree of reactivity to the peptide sequence. Other characteristics of 6193 MAB include: the ability to block opioid ligand binding in a radioreceptor assay; agonist activity similar to opioid peptides in suppressing cAMP production; and the recognition of a 58 kDa protein on the surface of the neuroblastoma x glioma cell line, NG108-15. These results are consistent with a reactivity of 6193 MAB with the delta-class opioid receptor.
Carr
D J
DJ
Department of Physiology and Biophysics, University of Alabama, Birmingham 35294.
Blalock
J E
JE
Bost
K L
KL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D002460:Cell Line; D002469:Cell Separation; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D004744:Enkephalin, Methionine / Q000276:immunology*; D005434:Flow Cytometry; D051379:Mice; D008807:Mice, Inbred BALB C; D009842:Oligopeptides / Q000276:immunology; D012313:RNA; D018075:RNA, Complementary; D011957:Receptors, Opioid / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D009842:Oligopeptides; D018075:RNA, Complementary; D011957:Receptors, Opioid; D004744:Enkephalin, Methionine; D012313:RNA; D000242:Cyclic AMP