[Results (in vitro and in vivo) of cryopreservation of human thrombocytes with glycerol].
The cryopreservation of human platelets was carried out with a final glycerol concentration of 0.38-0.48 mol/l (3.5-4.5%). The in-vitro-recovery (total platelets, aggregation, 14C-5-HT-uptake, clot retraction, total ATP, morphology) was in the range of 35-93% in comparison with freshly prepared platelet concentrates. The platelet survival was investigated by 51chrom labeling and autologous transfusion. A survival rate of 42% and a mean survival of 79% in comparison with freshly prepared platelets is concentrates are the results. In spite of reduced viability the cryopreservation of platelets is invaluable for providing compatible platelets for alloimmunized patients.
Zapff
G
G
Bezirksinstitut für Blutspende- und Transfusionswesen, Berlin.
Scheibe
I
I
Toursel
W
W
Dekowski
D
D
Richter
H
H
D001792:Blood Platelets / Q000187:drug effects; D001793:Blood Preservation / Q000379:methods*; D001803:Blood Transfusion; D002470:Cell Survival / Q000187:drug effects; D005615:Freezing; D005990:Glycerol / Q000008:administration & dosage*; D006801:Humans; D007231:Infant, Newborn; D010974:Platelet Aggregation / Q000187:drug effects; D010976:Platelet Count / Q000187:drug effects; D017713:Platelet Transfusion
D005990:Glycerol
Ergebnisse (in vitro und in vivo) der Kryokonservierung von Human-Thrombozyten mit Glycerol.