Modulation of the antigenicity of amylase in cervical glandular atypia, adenocarcinoma in situ and invasive adenocarcinoma.
Using a polyclonal anti-salivary alpha-amylase antibody and a biotin-avidin-peroxidase detection system immunoreactive amylase was detected in 39/40 cases of invasive cervical adenocarcinoma, 6/7 cases of adenocarcinoma in situ, 6/6 cases of high-grade cervical glandular atypia and 12/14 cases of low-grade cervical glandular atypia. Expression was seen in only 2/18 normal endocervices and this was weak and patchy. In contrast, strong amylase activity was demonstrated in 18/18 normal endocervices using a starch film assay on frozen sections. It is proposed that antigenic modulation of amylase occurs during endocervical neoplastic transformation.
Griffin
N R
NR
Department of Pathology, University of Leeds, UK.
Wells
M
M
Fox
H
H
D000230:Adenocarcinoma / Q000032:analysis / Q000201:enzymology* / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000681:Amylases / Q000276:immunology / Q000378:metabolism*; D000918:Antibody Specificity; D014408:Biomarkers, Tumor / Q000032:analysis; D002584:Cervix Uteri / Q000166:cytology / Q000201:enzymology / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D002583:Uterine Cervical Neoplasms / Q000032:analysis / Q000201:enzymology* / Q000473:pathology
D014408:Biomarkers, Tumor; D000681:Amylases