In situ staining of bromodeoxyuridine positive cells in normal and neoplastic colony-forming units grown on plasma clots.
Bromodeoxyuridine, an analogue of thymidine, can be detected by means of monoclonal antibodies and utilized as a marker of the S-phase of the cell cycle. In this paper a method for the detection of the labeling index of normal and neoplastic colony-forming units (CFU) growing in plasma clot semisolid medium is described and preliminary results on the cell cycle of 7th and 14th CFU granulocyte-macrophage are discussed.
Tazzari
P L
PL
Istituto di Ematologia Seràgnoli, Università di Bologna, Italy.
Gobbi
M
M
Tassi
C
C
Lemoli
R M
RM
Dinota
A
A
Visani
G
G
Tura
S
S
D001777:Blood Coagulation; D001973:Bromodeoxyuridine; D002449:Cell Aggregation; D002455:Cell Division; D002460:Cell Line; D002999:Clone Cells; D003114:Colony-Forming Units Assay; D006412:Hematopoietic Stem Cells / Q000032:analysis* / Q000473:pathology; D006801:Humans; D014411:Neoplastic Stem Cells / Q000032:analysis* / Q000473:pathology; D010949:Plasma; D013194:Staining and Labeling / Q000379:methods
D001973:Bromodeoxyuridine