Pigeon breeder's disease: the effect of antigen-specific antibodies on in vitro T cell responses of normal blood lymphocytes.
The effect(s) of affinity-purified antibodies to pigeon serum immunoglobulins on normal T cell responses in vitro was measured. Our results show that the antibodies partially inhibited the concanavalin-A-induced proliferation of normal lymphocytes (32% at 10 micrograms/ml concentration; p less than 0.001), whilst they had no significant effects at equivalent doses on the phytohemagglutinin-induced response. A study of the kinetics of this inhibition revealed that the antibodies exerted their effect(s) within the first 24 h of culture (p less than 0.001); this is probably due to their interference in early events intimately involved in the de novo synthesis and expression of activation antigens such as HLA-DR and Tac: antibody-treated cultures expressed 51.6 and 41.4% less HLA-DR and Tac, respectively. Two-colour immunofluorescence analysis showed that the specific antibodies bind to a subset of CD8+ cells only.
Bouic
P J
PJ
Department of Medical Microbiology, University of Stellenbosch, Tygerberg, South Africa.
de Beer
P M
PM
Daniels
J
J
Joubert
J R
JR
D000542:Alveolitis, Extrinsic Allergic / Q000276:immunology*; D000906:Antibodies / Q000502:physiology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D001716:Bird Fancier's Lung / Q000276:immunology*; D003208:Concanavalin A; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D007700:Kinetics; D008213:Lymphocyte Activation; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
D000906:Antibodies; D000945:Antigens, Differentiation, T-Lymphocyte; D000939:Epitopes; D003208:Concanavalin A