In vitro effects of growth factors on rat germ cell RNA synthesis and their modulation by Sertoli cell-secreted proteins.
In vitro rat germ cell RNA synthesis is influenced by growth factors. Basic fibroblast growth factor (0.1 to 100 ng/ml) increases [3H]uridine incorporation in round spermatids (RS) but not in pachytene spermatocytes (PS); this effect is potentiated by insulin (10 micrograms/ml) and blocked in the presence of Sertoli cell-secreted proteins (SCSP). Somatomedin C (0.1 to 100 ng/ml) exhibits a similar effect when used alone without an influence by SCSP. Transforming growth factor beta (0.1 to 10 ng/ml) acts on both cell types, but SCSP amplify this effect only in PS. These data suggest that growth factors synthesized in situ may play a role in the germ cell development and that their effects are modulated by SCSP.
Fradin
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S
U.A. CNRS 609, Faculté de Médecine, Caen, France.
Barbey
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P
Drosdowsky
M A
MA
D000818:Animals; D002478:Cells, Cultured; D005346:Fibroblast Growth Factors / Q000502:physiology; D006133:Growth Substances / Q000502:physiology*; D007334:Insulin-Like Growth Factor I / Q000502:physiology; D008297:Male; D012313:RNA / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D012708:Sertoli Cells / Q000378:metabolism*; D013094:Spermatozoa / Q000378:metabolism*; D015291:Transforming Growth Factors / Q000502:physiology
D006133:Growth Substances; D005346:Fibroblast Growth Factors; D012313:RNA; D007334:Insulin-Like Growth Factor I; D015291:Transforming Growth Factors