3'-Fluoro-3'-deoxyribonucleoside 5'-triphosphates: synthesis and use as terminators of RNA biosynthesis.
3'-Fluoro-3'-deoxy-uridine, -cytidine, -adenosine and -guanosine have been synthesized by glycosylation of the corresponding silylated bases with 1-O-acetyl-2,5-di-O-benzoyl-3-fluoro-3-deoxy-D-ribofuranose in the presence of Friedel-Crafts catalysts and were converted to the 5'-triphosphates, NTP(3'-F). It was shown that NTP(3'-F) are terminators of RNA synthesis catalyzed by DNA-dependent RNA polymerase from E. coli and may thus serve as tools for DNA sequencing.
Mikhailopulo
I A
IA
Institute of Bioorganic Chemistry, Byelorussian SSR Academy of Sciences, Minsk.
Pricota
T I
TI
Poopeiko
N E
NE
Sivets
G G
GG
Kvasyuk
E I
EI
Sviryaeva
T V
TV
Savochkina
L P
LP
Beabealashvilli
R S
RS
D001483:Base Sequence; D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D003854:Deoxyribonucleotides / Q000138:chemical synthesis* / Q000494:pharmacology; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000201:enzymology; D008969:Molecular Sequence Data; D012313:RNA / Q000037:antagonists & inhibitors / Q000096:biosynthesis*
D003854:Deoxyribonucleotides; D012313:RNA; D004259:DNA-Directed DNA Polymerase