[Stabilization of creatine kinase isoenzymes in the cerebrospinal fluid in cranio-cerebral trauma].
Stabilization with dithiothreitol, together with optimization of Mg2+ and EDTA concentrations in the reaction mixture and storage of the CSF samples for 24 hrs at +4 degrees C, has been carried out for the first time to improve the sensitivity of the technique for measuring the activities of creatine kinase (CK) and its isozymes in the CSF of 38 patients during the first 24 hrs of craniocerebral injury. Dithiothreitol promoted mostly an increase of the CK-BB isozyme; the content of this isozyme in the cerebral tissue is rather high, and it is considered as the cerebral tissue marker. Generally stabilization augmented the CSF total CK activity by 2.2 times on an average (from 50.6 to 113.2 U/l), and the CK-BB activity by 3.5 times on an average (from 21.6 to 74.8 U/l). The method used in this work will help improve the diagnostic sensitivity of the CSF CK-BB measurements, this being significant for the detection of the cerebral tissue minute injuries after traumas and neurosurgery.
Moshkin
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AV
D000293:Adolescent; D000328:Adult; D000368:Aged; D001930:Brain Injuries / Q000201:enzymology*; D003402:Creatine Kinase / Q000134:cerebrospinal fluid*; D004795:Enzyme Stability; D005260:Female; D006801:Humans; D007527:Isoenzymes; D008297:Male; D008875:Middle Aged
D007527:Isoenzymes; D003402:Creatine Kinase
Stabilizatsiia isofermentov kreatinkinazy tserebrospinal'noĭ zhidkosti pri cherepno-mozgovoĭ travme.