title: Distinct replicative and cytopathic characteristics of human immunodeficiency virus isolates.
abstract: According to their capacity to replicate in vitro, human immunodeficiency virus (HIV) isolates can be divided into two major groups, rapid/high and slow/low. Rapid/high viruses can easily be transmitted to a variety of cell lines of T-lymphoid (CEM, H9, and Jurkat) and monocytoid (U937) origin. In contrast, slow/low viruses replicate transiently, if at all, in these cell lines. Except for a few isolates, the great majority of slow/low viruses replicate in peripheral blood mononuclear cells and Jurkat-tatIII cells constitutively expressing the tatIII gene of HIV-1. The viruses able to replicate efficiently cause syncytium formation and are regularly isolated from immunodeficient patients. Poorly replicating HIV isolates, often obtained from individuals with no or mild disease, show syncytium formation and single-cell killing simultaneously or, with some isolates, cell killing only.
authors: Department of Virology, Karolinska Institutet, Stockholm, Sweden.
authors: E M
authors: EM
authors: Fenyö
authors: L
authors: L
authors: Morfeldt-Månson
authors: F
authors: F
authors: Chiodi
authors: B
authors: B
authors: Lind
authors: A
authors: A
authors: von Gegerfelt
authors: J
authors: J
authors: Albert
authors: E
authors: E
authors: Olausson
authors: B
authors: B
authors: Asjö
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000145:classification / Q000382:microbiology; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D006678:HIV / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D012194:RNA-Directed DNA Polymerase / Q000096:biosynthesis; D013045:Species Specificity; D014779:Virus Replication
chemical_list: D012194:RNA-Directed DNA Polymerase