Repair of bleomycin-induced DNA double-strand breaks in Vicia faba.
As detected by neutral DNA elution, bleomycin induced at the concentrations tested (5, 10 and 50 micrograms/ml) DNA double-strand breaks (dsbs) in in vitro cultured embryos of V. faba. Most of these breaks were repaired during a 4-h incubation period after treatment. Dsbs also occurred after treatment with 2.5 and 5 mM of N-methyl-N-nitrosourea (MNU) but in contrast to those induced by bleomycin, these dsbs remained unrepaired during the 4-h incubation period following the treatment.
Angelis
K J
KJ
Institute of Experimental Botany CSAV, Prague, Czechoslovakia.
Velemínský
J
J
Rieger
R
R
Schubert
I
I
D001761:Bleomycin / Q000633:toxicity*; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D004260:DNA Repair; D066298:In Vitro Techniques; D008770:Methylnitrosourea / Q000633:toxicity; D010944:Plants / Q000187:drug effects / Q000235:genetics*
D001761:Bleomycin; D008770:Methylnitrosourea; D004247:DNA