Quantitation of infectious human immunodeficiency virus using a modified plaque forming assay.
This report describes a modified plaque forming assay which has been used for quantitation of infectious human immunodeficiency virus (HIV) in cell culture supernatant fluid. The number of infectious units determined by the plaque assay correlates closely with the 50% infectious dose determined by a conventional assay in cell culture. In contrast, particle associated reverse transcriptase (RT) activity correlates poorly with the amount of infectious HIV present in culture supernatant fluids.
Kiernan
R E
RE
NHMRC Special Unit for AIDS Virology, Macfarlane Burnet Centre for Medical Research, Fairfield Hospital, Victoria, Australia.
McPhee
D A
DA
Doherty
R R
RR
D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D006678:HIV / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D010948:Viral Plaque Assay
D012194:RNA-Directed DNA Polymerase