[Kinetic characteristics of 22Na transport in human and rat erythrocytes during cytoplasm acidification and cell compression].
Under normal conditions (pH0 = 7.4, pHi = 7.1-7.2) amiloride, a Na+/H+ exchange inhibitor, does not influence Na+ intake by human and rat erythrocytes. Acidification of the cytoplasm (pHi approximately 6.4) is accompanied by the acceleration of 22Na intake, which is decreased after addition of 1 mM amiloride (by 50 and 80%, respectively). The Ki value of amiloride for human and rat erythrocytes is 30 and 250 microM, respectively. In rat erythrocytes the dependence of the rate of the delta pH-induced incorporation of 22Na on Na+ concentration is described by a saturation curve (K0.5 for Na0+ is approximately 40 mM), whereas in human erythrocytes it obeys the diffusion kinetics. These results suggest that the Na+/H+ exchange takes place in rat erythrocytes, but is absent in human erythrocytes. In rat erythrocytes the Na+/H+ exchange can be induced by cell compression which can be caused either by decreasing the KCl content (after addition of valinomycin) or by increasing the osmolarity of the medium (in the presence of sucrose). The rate of Na+/H+ exchange induced by cell compression is increased by 60-70% after addition of protein kinases A and C activators. No effect of intracellular Ca2+ on the rate of the Na+/H+ exchange in rat erythrocytes is observed.
Orlov
S N
SN
Pokudin
N I
NI
Riazhskiĭ
G G
GG
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects; D007700:Kinetics; D051381:Rats; D011921:Rats, Inbred WKY; D012964:Sodium / Q000097:blood*; D013045:Species Specificity; D014634:Valinomycin / Q000494:pharmacology
D007473:Ion Channels; D014634:Valinomycin; D000584:Amiloride; D012964:Sodium
Kineticheskie kharakteristiki transporta 22Na v éritrotsitakh cheloveka i krysa pri zakislenii tsitoplazmy i szhatii kletok.