Quantification of attached cells in tissue culture plates and on microcarriers.
A method for quantification of anchorage-dependent cells in culture on plane surfaces or on microcarriers is proposed. It is based on Coomassie brilliant blue R-250 adsorption, followed by elution of the dye and measurement by spectrophotometry at 595 nm. A linear correlation (r = 0.988 to 0.996) was observed between absorbance and cell number along a large range of cell densities. This technique may be used for monitoring cell growth, from seeding of initial inoculi to scaling up of cultures in bioreactors.
Margis
R
R
Departamento de Bioquímica, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Brazil.
Borojevic
R
R
D002452:Cell Count / Q000379:methods*; D002460:Cell Line; D002478:Cells, Cultured; D003124:Colorimetry; D003470:Culture Media; D005347:Fibroblasts; D012394:Rosaniline Dyes; D013194:Staining and Labeling; D014238:Trichloroacetic Acid
D003470:Culture Media; D012394:Rosaniline Dyes; D014238:Trichloroacetic Acid; C024757:Coomassie brilliant blue R