[Use of an affinity chromatography method for isolating monospecific Salmonella antibodies].
Obtaining antibodies to individual components of Salmonella antigenic complex is highly important for investigations aimed at the study of the antigenic structure of bacteria, their serological identification and the development of diagnostic preparations. The method of obtaining antibodies by the oxidation of Salmonella antigens with sodium periodate and creating immunosorbents based on these antibodies with subsequent affinity chromatography has been developed. Monospecific antibodies thus obtained (O2, O4, O9) have been studied and used as monospecific preparations in the agglutination test, the immunofluorescence test and the immunosorbent assay. The development of methods for stabilizing these preparations, thus ensuring their wide practical use, may be of interest.
Iankina
N F
NF
Shobukhova
T S
TS
Pavlova
I P
IP
Bogoiavlenskaia
L B
LB
Zykova
T A
TA
D000372:Agglutination Tests; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000302:isolation & purification*; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology; D002846:Chromatography, Affinity / Q000379:methods*; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D019081:O Antigens; D011817:Rabbits; D012475:Salmonella / Q000276:immunology*; D012482:Salmonella paratyphi A / Q000276:immunology
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D019081:O Antigens
Primenenie metoda affinnoĭ khromatografii dlia polucheniia monospetsificheskikh antitel k sal'monellam.