Uptake of fluorescence-labeled dextrans by 10T 1/2 fibroblasts following permeation by rectangular and exponential-decay electric field pulses.
The uptake of fluorescence-labeled dextrans by adherent 10T 1/2 murine fibroblasts following electric field pulse application was used as a criterion for the efficiency of electropermeation. The cells in monolayers were permeated by immersing a coaxial electrode in culture dishes. The percentage of cells which exhibited fluorescence uptake following electric field pulse application was measured at independently varying pulse field strength and pulse length. Dextrans with molecular weights equal to or higher than 41,000 dalton require higher field strength or longer pulse time to penetrate the cells. There is no detectable advantage of using a rectangular pulse against using an exponential decay pulse of similar power. The uptake was proportional to the product of the pulse amplitude and duration over the experimental range of 40-950 microseconds and 0.1-14.5 kV/cm. Cell survival decreases at the upper end of this range. The result provides a direct comparison of electric parameters which so far have not been standardized with regard to cell electropermeation.
Liang
H
H
ElectroCell, Inc., Buffalo, NY 14214.
Purucker
W J
WJ
Stenger
D A
DA
Kubiniec
R T
RT
Hui
S W
SW
D000818:Animals; D001693:Biological Transport, Active; D001709:Biotechnology; D002463:Cell Membrane Permeability; D003911:Dextrans / Q000493:pharmacokinetics*; D004560:Electricity; D005347:Fibroblasts / Q000378:metabolism*; D016650:Fluorescein-5-isothiocyanate; D005452:Fluoresceins; D008970:Molecular Weight; D013861:Thiocyanates
D003911:Dextrans; D005452:Fluoresceins; D013861:Thiocyanates; D016650:Fluorescein-5-isothiocyanate