Identification of a sulfoglycolipid epitope shared by cells of neuroectodermal and hematopoietic origin.
Monoclonal antibody (mAb) SNH.1 detects an epitope which is restricted to cells of neuroectodermal and hematopoietic origin. The mAb was obtained by immunization of a mouse with liposomes containing a crude extract of human melanoma acidic glycolipids. The SNH.1 antigen isolated from melanoma was identified as a sulfated glycolipid, closely related or identical to sulfogalactosyl-ceramide. When tested with different lipids, mAb SNH.1 reacted as well with other sulfoglycolipids. The staining of mAb SNH.1 is restricted to the cytoplasm and often localized to the perinuclear region. Therefore, the SNH.1 mAb epitope may be detectable only during the biosynthesis of sulfoglycolipids.
Schriever
F
F
Institute of Immunology, University of Munich, F.R.G.
Riethmüller
G
G
Johnson
J P
JP
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D002460:Cell Line; D002855:Chromatography, Thin Layer; D000939:Epitopes; D006017:Glycolipids / Q000276:immunology*; D006413:Hematopoietic System / Q000166:cytology* / Q000276:immunology; D007150:Immunohistochemistry; D008297:Male; D008545:Melanoma / Q000276:immunology; D051379:Mice; D009420:Nervous System / Q000166:cytology / Q000196:embryology* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006017:Glycolipids; C011117:sulfoglycolipids