title: Synthesis of alpha 1-acid glycoprotein by the human prostate.
abstract: In order better to define the extent of protein synthesis capacity of the human prostate, we have studied the translation of selected serum proteins using isolated poly(A)+ RNA preparations and the rabbit reticulocyte lysate system. The translation of alpha 1-acid glycoprotein could be conclusively demonstrated but there was no apparent translation of albumin and plasmatic transferrin. Labeled alpha 1-acid glycoprotein was identified by specific immunoprecipitation with a commercial anti alpha 1-acid glycoprotein antiserum and correct processing by canine pancreatic microsomal membranes. Furthermore, we have shown by the immunoperoxidase technique that alpha 1-acid glycoprotein was indeed localized mainly in prostatic epithelial cells in 2 out of 2 patients with benign prostatic hypertrophy and in 3 out of 11 patients with prostatic adenocarcinoma. The significance of the synthesis and secretion of alpha 1-acid glycoprotein by prostatic cell themselves is presently unknown. However, we think that it could represent an interesting subject to explore further in relation with prostatic inflammation.
authors: Laboratory of Hormonal Bioregulation, Laval University Hospital Centre, Ste-Foy, Québec, Canada.
authors: J Y
authors: JY
authors: Dubé
authors: G
authors: G
authors: Paradis
authors: B
authors: B
authors: Têtu
authors: R R
authors: RR
authors: Tremblay
mesh_terms: D002474:Cell-Free System; D004848:Epithelium / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D007425:Intracellular Membranes / Q000378:metabolism; D008297:Male; D009961:Orosomucoid / Q000096:biosynthesis*; D011467:Prostate / Q000378:metabolism*; D011470:Prostatic Hyperplasia / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012709:Serum Albumin / Q000235:genetics; D014168:Transferrin / Q000235:genetics
chemical_list: D009961:Orosomucoid; D012333:RNA, Messenger; D012709:Serum Albumin; D014168:Transferrin