Various human hematopoietic growth factors (interleukin-3, GM-CSF, G-CSF) stimulate clonal growth of nonhematopoietic tumor cells.
We have studied the effect of recombinant human hematopoietic growth factors (interleukin-3 [rhIL-3], granulocyte-macrophage colony-stimulating factor [rhGM-CSF], and granulocyte CSF [rhG-CSF]) on the clonal growth of human colon adenocarcinoma cell lines HTB-38, CCL 187, and WiDr (CCL 218). The factors stimulated clonal growth of HTB-38 and CCL 187 in a capillary modification of the human tumor clonogenic assay in agar up to twofold. There were dose-response correlations over a range of 1 to 10,000 U/mL for rhIL-3, rhGM-CSF, and rhG-CSF. Incubation with neutralizing monoclonal antibodies abolished the stimulation of clonal growth by rhGM-CSF. The WiDr cell line was nonresponsive to rhIL-3 and rhGM-CSF. These results represent the first evidence that a variety of hematopoietic growth factors can stimulate the growth of clonogenic cells of some nonhematopoietic malignant cell lines in vitro.
Berdel
W E
WE
Department of Medicine, Emory University School of Medicine, Atlanta, GA 30322.
Danhauser-Riedl
S
S
Steinhauser
G
G
Winton
E F
EF
D000230:Adenocarcinoma / Q000473:pathology; D000911:Antibodies, Monoclonal / Q000502:physiology; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002999:Clone Cells / Q000187:drug effects* / Q000473:pathology; D003110:Colonic Neoplasms / Q000473:pathology; D003115:Colony-Stimulating Factors / Q000276:immunology; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000276:immunology; D006801:Humans; D007377:Interleukin-3; D014411:Neoplastic Stem Cells / Q000187:drug effects* / Q000473:pathology; D009500:Neutralization Tests; D011994:Recombinant Proteins / Q000276:immunology / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000473:pathology
D000911:Antibodies, Monoclonal; D003115:Colony-Stimulating Factors; D006133:Growth Substances; D007377:Interleukin-3; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor