Detection of genes of interest in tissues and cells by in situ hybridization.
In situ hybridization (ISH) is a powerful technique for assessing the expression of particular genes of interest within individual cells in tissues or cytospins. The only limitations of the technique are the availability of appropriate probes and tissues. When coupled with routine histology and DNA analysis, characterization and classification of tissues can be greatly extended. Future applications include the identification of tumor-specific markers that can be combined with ISH in diagnostic strategies.
Seibel
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NL
National Cancer Institute, Navy Medical Oncology Branch, Bethesda, Maryland 20814.
Smith-Gill
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Hollis
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GF
Kirsch
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IR
D000818:Animals; D004247:DNA / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis; D005786:Gene Expression Regulation; D006801:Humans; D009369:Neoplasms / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011519:Proto-Oncogenes; D012313:RNA / Q000032:analysis*; D012334:RNA, Neoplasm / Q000032:analysis
D004273:DNA, Neoplasm; D012334:RNA, Neoplasm; D012313:RNA; D004247:DNA