Commitment of mouse fibroblasts to adipocyte differentiation by DNA transfection.
Cells of the mouse cell line 3T3-F442A can be induced by various hormones to differentiate into adipocytes, whereas cells of 3T3-C2, a subclone of 3T3, cannot. However, transfection of DNA from uninduced 3T3-F422A cells into 3T3-C2 cells permits recovery of 3T3-C2 transfectants that differentiate into adipocytes in the presence of insulin. DNA isolated from human fat tissue, when transfected into 3T3-C2 mouse cells, also gives rise to mouse transfectants that are induced to differentiate into adipocytes by the addition of insulin. Apparently, transfection of a trans-regulatory gene (or genes) from 3T3-F442A or human fat cells into 3T3-C2 cells is sufficient to commit 3T3-C2 cells to adipocyte differentiation.
Chen
S
S
Department of Biological Sciences, Columbia University, New York, NY 10027.
Teicher
L C
LC
Kazim
D
D
Pollack
R E
RE
Wise
L S
LS
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D004262:DNA Restriction Enzymes; D003907:Dexamethasone / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology*; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D051379:Mice; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis; D014162:Transfection
D015342:DNA Probes; D007328:Insulin; D012333:RNA, Messenger; D003907:Dexamethasone; D004247:DNA; D005993:Glycerolphosphate Dehydrogenase; D004262:DNA Restriction Enzymes; D015056:1-Methyl-3-isobutylxanthine