title: Chronic lymphocytic leukemic (CLL) cells secrete multispecific autoantibodies.
abstract: A subset of B cells expressing the CD5 marker, a 67 KD molecule, has been implicated in the pathogenesis of autoimmune disease. To study the immunoglobulin repertoire of CD5+ B cells we investigated chronic lymphocytic leukemic (CLL) cells, since the majority of the malignant clones express CD5. CLL were induced to secrete their IgM in vitro by phorbol 12-myristate 13-acetate (PMA) and the supernatants screened for binding to a panel of autoantigens. Twelve out of 14 CLL clones were autoreactive, binding to Fc of IgG, ssDNA, dsDNA, histones, cardiolipin, or cytoskeletal components. Many also bound to more than one antigen tested for, showing multispecificity. Our data suggest that a high proportion of CD5+ B cells are programmed to secrete multispecific autoantibodies.
authors: Department of Immunology, University College and Middlesex School of Medicine, London, UK.
authors: B M
authors: BM
authors: Bröker
authors: A
authors: A
authors: Klajman
authors: P
authors: P
authors: Youinou
authors: J
authors: J
authors: Jouquan
authors: C P
authors: CP
authors: Worman
authors: J
authors: J
authors: Murphy
authors: L
authors: L
authors: Mackenzie
authors: R
authors: R
authors: Quartey-Papafio
authors: M
authors: M
authors: Blaschek
authors: P
authors: P
authors: Collins
mesh_terms: D000912:Antibodies, Neoplasm / Q000276:immunology*; D000918:Antibody Specificity; D000943:Antigens, Differentiation / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis; D001323:Autoantibodies / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000032:analysis; D018956:CD5 Antigens; D006801:Humans; D007147:Immunoglobulin Light Chains / Q000032:analysis; D007075:Immunoglobulin M / Q000276:immunology*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000276:immunology* / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000276:immunology
chemical_list: D000912:Antibodies, Neoplasm; D000943:Antigens, Differentiation; D000951:Antigens, Neoplasm; D001323:Autoantibodies; D001324:Autoantigens; D014408:Biomarkers, Tumor; D018956:CD5 Antigens; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M