Insertion sequence IS10 anti-sense pairing initiates by an interaction between the 5' end of the target RNA and a loop in the anti-sense RNA.
Transposition of insertion sequence IS10 is regulated by an anti-sense RNA which inhibits transposase expression when IS10 is present in multiple copies per cell. The anti-sense RNA (RNA-OUT) consists of a stem domain topped by a flexibly paired loop; the 5' end of the target molecule, RNA-IN, is complementary to the top of the loop, and complementarity extends for 35 base-pairs down one side of RNA-OUT. We present here genetic evidence that anti-sense pairing, both in vitro and in vivo, initiates by interaction of the 5' end of RNA-IN and the loop domain of RNA-OUT; other features of the reaction are discussed. In the context of this model, we discuss features of this anti-sense system which are important for its biological effectiveness, and suggest that IS10 provides a convenient model for design of efficient artificial anti-sense RNA molecules.
Kittle
J D
JD
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.
Simons
R W
RW
Lee
J
J
Kleckner
N
N
D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D006860:Hydrogen Bonding; D066298:In Vitro Techniques; D009693:Nucleic Acid Hybridization; D009713:Nucleotidyltransferases / Q000378:metabolism*; D012313:RNA / Q000235:genetics* / Q000648:ultrastructure; D016372:RNA, Antisense; D012329:RNA, Bacterial / Q000235:genetics* / Q000648:ultrastructure; D012260:Ribonucleases / Q000378:metabolism; D013329:Structure-Activity Relationship; D019895:Transposases
D004251:DNA Transposable Elements; D016372:RNA, Antisense; D012329:RNA, Bacterial; D012313:RNA; D009713:Nucleotidyltransferases; D019895:Transposases; D012260:Ribonucleases