title: Opsin- and S-antigen-like immunoreactions in photoreceptors of the tree shrew retina.
abstract: In the tree shrew retina individual rod and cone photoreceptors can be readily identified and quantified because their perikarya are arranged in a single layer. This retina is therefore an ideal system for testing the specificity of photoreceptor-directed antibodies. Here we describe the staining properties of polyclonal antibodies against (rhod)opsin and retinal S-antigen in the tree shrew retina. The (rhod)opsin antibody exclusively and completely labelled the rod population. The antibody against S-antigen also labelled all rods and, in addition, a regularly arrayed subpopulation of cones, which we argue to be the blue-sensitive cones. In the context of our findings, the labelling of pinealocytes with these antibodies is discussed.
authors: Max-Planck-Institut für Hirnforschung, Frankfurt, Federal Republic of Germany.
authors: B
authors: B
authors: Müller
authors: L
authors: L
authors: Peichl
authors: W J
authors: WJ
authors: De Grip
authors: I
authors: I
authors: Gery
authors: H W
authors: HW
authors: Korf
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D000941:Antigens / Q000276:immunology*; D019393:Arrestin; D005136:Eye Proteins / Q000276:immunology*; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D010786:Photoreceptor Cells / Q000276:immunology*; D012160:Retina / Q000166:cytology / Q000276:immunology* / Q000648:ultrastructure; D012243:Rhodopsin / Q000276:immunology; D017299:Rod Opsins; D013194:Staining and Labeling; D014418:Tupaiidae
chemical_list: D000906:Antibodies; D000941:Antigens; D019393:Arrestin; D005136:Eye Proteins; D017299:Rod Opsins; D012243:Rhodopsin