Two human myeloma cell lines, amylase-producing KMS-12-PE and amylase-non-producing KMS-12-BM, were established from a patient, having the same chromosome marker, t(11;14)(q13;q32).
Two human myeloma cell lines, KMS-12-PE and KMS-12-BM, were established from a 64-year-old woman with a non-producing type of multiple myeloma. The KMS-12-PE line originated from the pleural effusion and the KMS-12-BM from the bone marrow. These two lines showed the same chromosome marker, t(11:14)(q13:q32). However, their phenotypes of surface markers differed from each other. KMS-12-BM cells were positive to CD20, CD38 and PCA-1. showing the plasmacytoid (immature plasma cell) stage of B-cell differentiation, while KMS-12-PE cells were positive to CD38 and PCA-1, but not to CD20, indicating the terminal differentiated stage of B-cells. As seen in the pleural effusion of the patient. KMS-12-PE cells ectopically produced a salivary type of amylase, but KMS-12-BM cells did not. Interestingly, the chromosome abnormality of del(1)(p22----pter) near the region of 1p21, where the amylase gene was assigned, was noticed in as many as 76% of KMS-12-PE cells.
Ohtsuki
T
T
Department of Medicine, Kawasaki Medical School, Kurashiki, Japan.
Yawata
Y
Y
Wada
H
H
Sugihara
T
T
Mori
M
M
Namba
M
M
D000681:Amylases / Q000096:biosynthesis*; D000951:Antigens, Neoplasm / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D001853:Bone Marrow / Q000473:pathology; D002460:Cell Line / Q000201:enzymology; D002880:Chromosomes, Human, Pair 11; D002883:Chromosomes, Human, Pair 14; D005260:Female; D005819:Genetic Markers; D006801:Humans; D007621:Karyotyping; D008875:Middle Aged; D009101:Multiple Myeloma / Q000201:enzymology* / Q000235:genetics; D010996:Pleural Effusion / Q000473:pathology; D014178:Translocation, Genetic
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D005819:Genetic Markers; D000681:Amylases