[A photographic microfluorimetric method for determining the DNA content in individual human chromosomes].
A method for determination of DNA contents in individual human chromosomes has been elaborated based of the two step analysis: 1) identification of chromosomes by Q-banding; 2) photographic microfluorimetry of chromosomes after the Feulgen staining (a fluorescent variant using a Schiff-type reagent of Auramine-SO2). The DNA content in 24 human chromosomes was calculated in absolute values (fg). The data obtained are compared with the evidence published elsewhere and provided by different cytophotometric methods.
Sukhikh
T R
TR
Kalimagambetov
A M
AM
Barskiĭ
I Ia
IIa
Khomenkova
S A
SA
Vinogradov
A E
AE
D000818:Animals; D002478:Cells, Cultured; D002871:Chromosome Banding / Q000379:methods; D002877:Chromosomes, Human / Q000032:analysis*; D004396:Coloring Agents; D003592:Cytophotometry / Q000379:methods*; D004247:DNA / Q000032:analysis*; D006801:Humans; D008214:Lymphocytes / Q000032:analysis; D008677:Metaphase; D010781:Photography / Q000379:methods*; D012394:Rosaniline Dyes; D013194:Staining and Labeling / Q000379:methods
D004396:Coloring Agents; C007722:Feulgen stain; D012394:Rosaniline Dyes; D004247:DNA
Fotograficheskiĭ mikrofluorimetricheskiĭ metod opredeleniia soderzhaniia DNK v individual'nykh khromosomakh cheloveka.