Interleukin 3 binds to a 140-kDa phosphotyrosine-containing cell surface protein.
Interleukin 3 (IL-3) is a hematopoietic growth factor that supports the proliferation and differentiation of early hematopoietic-lineage progenitors. By using growth factor-dependent cell lines, IL-3 has been shown to induce the appearance of several phosphotyrosine-containing proteins, including a 140-kDa cell surface phosphoprotein. Here we demonstrate that the 140-kDa phosphoprotein binds IL-3 and forms a stable complex with an apparent molecular mass of 170 kDa. The possible relationship of the 140-kDa phosphoprotein to the previously described 65-kDa IL-3-binding protein is discussed.
Isfort
R J
RJ
Molecular Mechanisms of T Cell Leukemogenesis Section, National Cancer Institute-Frederick Cancer Research Facility, MD 21701.
Stevens
D
D
May
W S
WS
Ihle
J N
JN
D000345:Affinity Labels / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D007377:Interleukin-3 / Q000378:metabolism*; D008970:Molecular Weight; D019000:Phosphotyrosine; D011971:Receptors, Immunologic / Q000378:metabolism*; D016185:Receptors, Interleukin-3; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis
D000345:Affinity Labels; D007377:Interleukin-3; D011971:Receptors, Immunologic; D016185:Receptors, Interleukin-3; D019000:Phosphotyrosine; D014443:Tyrosine