Comparison of some recent methods for the differentiation of elevated serum amylase and the detection of macroamylasaemia.
A pancreatic isoamylase method (Pancreatic Alpha-Amylase EPS, Boehringer) that uses monoclonal antibodies showed almost complete immunoinhibition of salivary (S) amylase activity with only a minor decrease of pancreatic (P) amylase activity. The method displayed good sensitivity and linearity. The correlations of P-amylase activities determined by this technique with a wheat-germ inhibition method and with agarose electrophoresis followed by densitometric scanning were excellent. However, both the wheat-germ and monoclonal inhibition methods failed to detect macroamylasaemia. To recognise macroamylases we used the PEG precipitation method and confirmed the results with agarose electrophoresis. Of 161 serum samples with elevated amylase activities, only four out of five with macroamylasaemia were detected by the PEG precipitation method. No false positives were demonstrated. After PEG precipitation of 28 samples, P-amylase determinations were performed on the supernatants. Again, four out of five with macroamylasaemia were recognised. We consider P-amylase measurement and, when macroamylasaemia is suspected, the combined use of the PEG precipitation method and P-amylase or total amylase determination to be the most practical way to differentiate between elevated serum amylase levels.
Van Deun
A
A
Department of Clinical Chemistry, University Hospital, Katholieke Universiteit Leuven, Belgium.
Cobbaert
C
C
Van Orshoven
A
A
Claeys
G
G
Lissens
W
W
D000681:Amylases / Q000097:blood*; D011232:Chemical Precipitation; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007527:Isoenzymes / Q000097:blood; D046911:Macromolecular Substances; D010189:Pancreatic Juice / Q000201:enzymology; D011092:Polyethylene Glycols; D012463:Saliva / Q000201:enzymology
D007527:Isoenzymes; D046911:Macromolecular Substances; D011092:Polyethylene Glycols; D000681:Amylases; C001663:macroamylase