title: T-immunogenic peptides are constituted of rare sequence patterns. Use in the identification of T epitopes in the human immunodeficiency virus gag protein.
abstract: The sequences of a set of 63 peptides of demonstrated T immunogenicity have been analyzed and compared with two different randomly generated sets of sequences. This study indicates a statistically significant tendency of T immunogenic peptides to be constituted of clusters of rare tetrapeptides, as evaluated from the available sequence data banks. This result has been used to locate potential T epitopes in the human immunodeficiency virus (HIV) gag protein. Four peptides corresponding to the best candidate T epitopes (chosen in regions of conserved sequence among different virus isolates) have been synthesized and found to be recognized by a HIV-1-specific, HLA-A2-restricted human cytotoxic T cell line.
authors: Unité d'Informatique Scientifique, Institut Pasteur, Paris, France.
authors: J M
authors: JM
authors: Claverie
authors: P
authors: P
authors: Kourilsky
authors: P
authors: P
authors: Langlade-Demoyen
authors: A
authors: A
authors: Chalufour-Prochnicka
authors: G
authors: G
authors: Dadaglio
authors: F
authors: F
authors: Tekaia
authors: F
authors: F
authors: Plata
authors: L
authors: L
authors: Bougueleret
mesh_terms: D000595:Amino Acid Sequence; D000939:Epitopes; D015683:Gene Products, gag; D006678:HIV / Q000276:immunology*; D015488:HIV Antigens / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007256:Information Systems; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology; D012191:Retroviridae Proteins / Q000235:genetics / Q000276:immunology*; D013223:Statistics as Topic; D013329:Structure-Activity Relationship; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000939:Epitopes; D015683:Gene Products, gag; D015488:HIV Antigens; D010455:Peptides; D012191:Retroviridae Proteins