New monoclonal antibodies recognizing epidermal differentiation-associated keratins in formalin-fixed, paraffin-embedded tissue. Keratin 10 expression in carcinoma of the vulva.
Two monoclonal antibodies (MAb) specific for differentiation-related epidermal keratins have been developed. They represent specific molecular probes for different stages of epidermal differentiation. Antibody DE-K10 is chain-specific for cytokeratin polypeptide no. 10 (56.5 kD) expressed in all suprabasal layers of the epidermis. Antibody DE-SCK is specific for modified stratum corneum keratins and thus represents a marker for the terminal step of epidermal differentiation. Since the epitopes identified by both antibodies are preserved in formalin-fixed, paraffin-embedded tissue sections, these antibodies can be used for retrospective studies of differentiation in various pathological processes. We have used antibody DE-K10 to study the cytokeratin 10 expression in 26 stage II or III vulvar squamous cell carcinomas. Preliminary data suggest an increased risk of recurrence in cytokeratin 10 negative tumours.
Ivanyi
D
D
Division of Tumour Biology, The Netherlands Cancer Institute, Amsterdam.
Ansink
A
A
Groeneveld
E
E
Hageman
P C
PC
Mooi
W J
WJ
Heintz
A P
AP
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D002294:Carcinoma, Squamous Cell / Q000032:analysis; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D004817:Epidermis / Q000032:analysis; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007633:Keratins / Q000032:analysis*; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D014846:Vulvar Neoplasms / Q000032:analysis* / Q000473:pathology
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D007633:Keratins