[Increased expression of IGF-2 in tumor tissue of nephroblastoma].
The incidence of nephroblastoma is increased in a number of syndromes with abnormal growth pattern. Elevated IGF 2-expression has been documented in various Wilms' tumors and a defect in the IGF 2 gene has been noted in 1 case. Southern blot analysis of genomic DNA of nephroblastomas in 5 additional patients after restriction enzyme digest with EcoRI, Pvu II, Pst I and Taq I did not reveal any defect in the IGF 2 gene. Slot blot analysis however showed marked overexpression of IGF 2-mRNA reaching up to more than 25 times the level expressed in unaffected kidney tissue adjacent to the tumor and in normal kidney tissue used as controls.
Briner
J
J
Hassam
S
S
Schönle
E J
EJ
Rohrer
U
U
D015139:Blotting, Southern; D002880:Chromosomes, Human, Pair 11; D004273:DNA, Neoplasm / Q000235:genetics; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000235:genetics*; D007680:Kidney Neoplasms / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D013002:Somatomedins / Q000235:genetics*; D009396:Wilms Tumor / Q000235:genetics*
D004273:DNA, Neoplasm; D013002:Somatomedins; D007335:Insulin-Like Growth Factor II
Nachweis erhöhter Expression von IGF 2 im Tumorgewebe von Nephroblastomen.