Ultrastructural localizations of beta-microseminoprotein, a prostate-specific antigen, in human prostate and sperm: comparison with gamma-seminoprotein, another prostate-specific antigen.
Immunohistochemical localizations of beta-microseminoprotein and gamma-seminoprotein, which are prostate-specific antigens, were examined by light and electron microscopy with peroxidase-labeled antibody. In normal, hypertrophic, and neoplastic prostate glands, beta-microseminoprotein was found in glandular epithelium but not in stroma cells. beta-Microseminoprotein may be as useful as gamma-seminoprotein in the pathologic examination of prostatic diseases, especially in histogenic classification of tumors or metastatic tumors. In an immunoelectron microscopic study, the primary localizations of beta-microseminoprotein and gamma-seminoprotein in the cell were demonstrated to be secretory granule and lysosome, respectively, suggesting that beta-microseminoprotein is a secretory protein. Weak distributions of beta-microseminoprotein and gamma-seminoprotein were observed in rough endoplasmic reticulum and Golgi apparatus. In spermatozoa, beta-microseminprotein was found attached to the cell membrane of the head but not in the tail, and gamma-seminoprotein was not found at all.
Ito
Y
Y
Department of Legal Medicine, Kurume University School of Medicine, Fukuoka, Japan.
Tsuda
R
R
Kimura
H
H
D000951:Antigens, Neoplasm / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008854:Microscopy, Electron; D011467:Prostate / Q000648:ultrastructure*; D011470:Prostatic Hyperplasia / Q000473:pathology; D011471:Prostatic Neoplasms / Q000473:pathology; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000032:analysis* / Q000276:immunology; D029589:Seminal Plasma Proteins; D013094:Spermatozoa / Q000648:ultrastructure*
D000951:Antigens, Neoplasm; D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein