The role of proteinases, interferons and hormones in proliferative activities of nonmalignant and malignant cells.
Proliferative responses, in form of colony forming ability, of nonmalignant Chinese hamster fibroblasts (V79) and malignant human laryngeal carcinoma cells (HEp) were examined after treatment with proteinases, proteinase inhibitors, interferons or hormones, in G0-phase, early G1-phase and G1/S point of the cell cycle. These phases of the cell cycle are of particular importance from the point of control and regulation of the proliferative activity of cells, and the effects of proteinases and other agents examined in this study occurred predominantly at these points. Among the agents tested, cysteine proteinase inhibitor (CPI) and calf liver neutral proteinase acted differently on malignant cells as compared to nonmalignant cells: they inhibited the proliferative commitment of malignant HEp cells in early G1-phase, while stimulated colony formation of V79 cells at the same point of the cell cycle.
Korbelik
M
M
Department of Experimental Biology and Medicine, Ruder Mosković Institute, Zagreb, Yugoslavia.
Skrk
J
J
Suhar
A
A
Turk
V
V
D000818:Animals; D002453:Cell Cycle; D002455:Cell Division / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D010450:Endopeptidases / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects; D006728:Hormones / Q000494:pharmacology*; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D007822:Laryngeal Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
D006728:Hormones; D007372:Interferons; D010450:Endopeptidases