Expression of immunogenic epitopes of hepatitis B surface antigen with hybrid flagellin proteins by a vaccine strain of Salmonella.
A nonvirulent Salmonella dublin flagellin-negative, aromatic-dependent live vaccine strain has been used to express hepatitis B virus surface antigen epitopes in an immunogenic form. The envelope proteins of the virion are encoded by the S gene, which contains the pre-S1, pre-S2, and S coding regions. Synthetic oligonucleotides corresponding to amino acid residues S-(122-137) and pre-S2-(120-145) were inserted in-frame into the hypervariable region of a cloned Salmonella flagellin gene, and the recombinant plasmids were introduced into a flagellin-negative aroA mutant live vaccine strain of S. dublin, SL5928. The flagellin gene was expressed in bacteria carrying the plasmids as detected by immunoblotting with anti-flagellin (H1-d) serum. Both the S and pre-S2 epitopes were detected in bacteria carrying the relevant plasmid by immunoblotting with anti-HBs (antibody to hepatitis B virus surface antigen) and anti-peptide antisera. Animals immunized intramuscularly or orally with the live recombinant bacteria developed antibodies specific to these hepatitis B virus epitopes as detected by ELISA.
Wu
J Y
JY
Department of Medicine, Stanford-University School of Medicine, CA 94305.
Newton
S
S
Judd
A
A
Stocker
B
B
Robinson
W S
WS
D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D000939:Epitopes / Q000235:genetics* / Q000276:immunology; D005408:Flagellin / Q000235:genetics*; D006514:Hepatitis B Surface Antigens / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D010957:Plasmids; D012475:Salmonella / Q000235:genetics*; D014765:Viral Vaccines / Q000276:immunology
D001426:Bacterial Proteins; D000939:Epitopes; D006514:Hepatitis B Surface Antigens; D014765:Viral Vaccines; D005408:Flagellin