Identification of a novel bone marrow-derived B-cell progenitor population that coexpresses B220 and Thy-1 and is highly enriched for Abelson leukemia virus targets.
A novel stage in early B-lymphocyte differentiation has been identified in normal mouse bone marrow cells. Earlier work had demonstrated that bone marrow cells characterized by low levels of Thy-1 and lack of a panel of lineage markers (Thy-1lo Lin- cells) were highly enriched for pluripotent hematopoietic stem cells. In this paper, we present evidence that another bone marrow population, which expressed low levels of Thy-1 and coexpressed B220, a B-lineage-specific form of the leukocyte common antigen, contained early and potent precursors for B lymphocytes upon in vivo transfer to irradiated hosts. These Thy-1lo B220+ cells, comprising 1 to 2% of bone marrow cells, were enriched for large cells in the mitotic cycle; the population lacked significant pluripotent hematopoietic stem cell activity and myeloid-erythroid progenitors. Most strikingly, Thy-1lo B220+ cells represented a highly enriched population of bone marrow cells that could be targets of Abelson murine leukemia virus transformation. We propose that Thy-1lo B220+ bone marrow cells represent the earliest stage of committed lymphocyte progenitors, intermediate in differentiation between Thy-1lo Lin- pluripotent stem cells and, in the B lineage, Thy-1- B220+ pre-B cells.
Tidmarsh
G F
GF
Department of Pathology, Stanford University School of Medicine, California 94305.
Heimfeld
S
S
Whitlock
C A
CA
Weissman
I L
IL
Müller-Sieburg
C E
CE
D000011:Abelson murine leukemia virus / Q000502:physiology*; D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000096:biosynthesis; D000954:Antigens, Surface / Q000096:biosynthesis; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000382:microbiology; D001854:Bone Marrow Cells; D002453:Cell Cycle; D002460:Cell Line; D002472:Cell Transformation, Viral; D005455:Fluorescent Antibody Technique; D015321:Gene Rearrangement; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology* / Q000382:microbiology; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D009052:Leukemia Virus, Murine / Q000502:physiology*; D017493:Leukocyte Common Antigens; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype; D018800:Thy-1 Antigens
D000944:Antigens, Differentiation, B-Lymphocyte; D000954:Antigens, Surface; D007143:Immunoglobulin Heavy Chains; D018800:Thy-1 Antigens; D017493:Leukocyte Common Antigens