Binding of deoxyadenylate and deoxycytidylate antibodies to double-stranded DNA.
Antibodies raised against deoxyadenylate and deoxycytidylate were found to react with double stranded DNA as assessed by highly sensitive avidin-biotin microELISA. The binding was specific as it was completely inhibited by the homologous hapten. The antibodies did not react with tRNA and rRNA. These antibodies were also shown to react with supercoiled and relaxed forms of pBR322 DNA as demonstrated by gel retardation assay.
Vaishnav
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Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore.
Antony
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A
D000906:Antibodies; D000936:Antigen-Antibody Complex; D004247:DNA / Q000276:immunology; D003838:Deoxyadenine Nucleotides / Q000276:immunology*; D003843:Deoxycytidine Monophosphate / Q000276:immunology*; D003845:Deoxycytosine Nucleotides / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D007700:Kinetics; D008832:Microchemistry
D000906:Antibodies; D000936:Antigen-Antibody Complex; D003838:Deoxyadenine Nucleotides; D003845:Deoxycytosine Nucleotides; D000939:Epitopes; D003843:Deoxycytidine Monophosphate; C012873:2'-deoxy-5'-adenosine monophosphate; D004247:DNA