Characterization of two genes encoding antigenically distinct type-1 fimbriae of Klebsiella pneumoniae.
A uropathogenic isolate of Klebsiella pneumoniae was shown to exhibit a mannose-sensitive hemagglutinating phenotype and to produce type-1 fimbriae consisting of subunits with a different electrophoretic mobility than those previously investigated. The gene cluster encoding expression of fimbriae was cloned and the genetic organization of the encoded polypeptides was determined. The gene encoding the major fimbrial subunit was localized and further examined by nucleotide sequence analysis. Comparison of two K. pneumoniae fimbrial genes revealed a nucleotide sequence agreement of 73%, and amino acid sequence agreement of 84% for the mature fimbrial subunits. Predictions of putative antigenic sites were correlated with regions demonstrating amino acid variability. In agreement with these predictions, no serological cross-reactivity between both fimbrial proteins could be demonstrated using an enzyme-linked immunosorbent assay (ELISA).
Gerlach
G F
GF
Department of Microbiology, University of Iowa, Iowa City 52242.
Clegg
S
S
D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000032:analysis*; D005796:Genes; D005798:Genes, Bacterial; D007711:Klebsiella pneumoniae / Q000235:genetics*; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping
D000942:Antigens, Bacterial; D000939:Epitopes; C022924:colonization factor antigens; D036781:Fimbriae Proteins; D004262:DNA Restriction Enzymes