Tumor necrosis factor-alpha and tumor necrosis factor-beta (lymphotoxin) stimulate the production of granulocyte-macrophage colony-stimulating factor, macrophage colony-stimulating factor, and IL-1 in vivo.
A number of cell types have been shown to elaborate hematopoietic growth factors in response to inflammatory mediators in vitro. To determine if this response occurs in vivo, we have administered levels of TNF-alpha and TNF-beta (lymphotoxin) found during an inflammatory reaction to mice. Using Northern blot analysis to detect tissue levels of hematopoietic growth factor-specific transcripts, and specific biologic and immunologic assays to detect the presence of colony-stimulating factors in the serum, we have found that TNF-alpha and TNF-beta induce the transcription and production of granulocyte-macrophage-CSF, macrophage-CSF, and IL-1. These findings provide an in vivo mechanism for the hematopoietic response to inflammation.
Kaushansky
K
K
Division of Hematology, University of Washington, School of Medicine, Seattle 98195.
Broudy
V C
VC
Harlan
J M
JM
Adamson
J W
JW
D000818:Animals; D015152:Blotting, Northern; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000096:biosynthesis* / Q000302:isolation & purification; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000096:biosynthesis* / Q000302:isolation & purification; D006410:Hematopoiesis / Q000187:drug effects; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D008233:Lymphotoxin-alpha / Q000008:administration & dosage*; D051379:Mice; D008809:Mice, Inbred C3H; D012313:RNA / Q000302:isolation & purification; D014158:Transcription, Genetic; D014409:Tumor Necrosis Factor-alpha / Q000008:administration & dosage*
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D007375:Interleukin-1; D008233:Lymphotoxin-alpha; D014409:Tumor Necrosis Factor-alpha; D012313:RNA; D016178:Granulocyte-Macrophage Colony-Stimulating Factor