Frequencies of micronuclei detected on Mytilus galloprovincialis by different staining techniques after treatment with zinc chloride.
The frequencies of micronuclei induced by ZnCl2 and detected on the gill tissue of the marine mussel Mytilus galloprovincialis with different staining techniques (acridine orange, gallocyanin chromallum, Feulgen, Giemsa) were compared. At least in the used system, the Feulgen and gallocyanin chromallum methods gave a frequency of micronuclei significantly lower than that obtained with the acridine orange and Giemsa techniques. No significant difference between the frequencies obtained with acridine orange and Giemsa was shown. So, though the acridine orange is surely the method which provides the more reliable data, in environmental screening works the Giemsa technique may be more suitable for its simplicity.
Majone
F
F
Department of Biology, University of Padua, Italy.
Beltrame
C
C
Brunetti
R
R
D000818:Animals; D049872:Bivalvia / Q000187:drug effects*; D002467:Cell Nucleus / Q000187:drug effects; D002712:Chlorides / Q000494:pharmacology*; D009152:Mutagenicity Tests; D013194:Staining and Labeling; D015032:Zinc / Q000494:pharmacology*; D017967:Zinc Compounds
D002712:Chlorides; D017967:Zinc Compounds; C016837:zinc chloride; D015032:Zinc