title: [Microsurgically collected living inner hair cells allow measurements of ion channels and cell potential].
abstract: Living inner hair cells of the mammalian inner ear were dissected microsurgically from guinea-pig cochleae in the absence of proteolytic enzymes. They were kept in short-time culture for 2 hours. Viability of the preparation was monitored by registration of the cell potential with impaled microelectrodes. The patch-clamp technique allowed the identification of 3 types of ionic channels in the basolateral cell membrane. Isolated living inner hair cells are a new model allowing to investigate the cellular basis of physiological function and pathophysiological disorders of sound perception.
authors: Universitätsklinik und Poliklinik für Hals-Nasen-Ohren-Kranke, Würzburg.
authors: A H
authors: AH
authors: Gitter
authors: H P
authors: HP
authors: Zenner
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods; D002470:Cell Survival; D005260:Female; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000502:physiology*; D006199:Hair Cells, Auditory, Inner / Q000502:physiology*; D007473:Ion Channels / Q000502:physiology*; D008297:Male; D008564:Membrane Potentials; D008866:Microsurgery / Q000379:methods
chemical_list: D007473:Ion Channels
vernacular_title: Mikrochirurgisch gewonnene lebende innere Haarzellen erlauben Messungen von Ionenkanälen und Zellpotential.