title: NMR study of the interactions of polymyxin B, gramicidin S, and valinomycin with dimyristoyllecithin bilayers.
abstract: The interactions of three polypeptide antibiotics (polymyxin B, gramicidin S, and valinomycin) with artificial lecithin membranes were studied by nuclear magnetic resonance (NMR). Combination of 31P and 2H NMR allowed observation of perturbations of the bilayer membrane structure induced by each of the antibiotics in the regions of the polar headgroups and acyl side chains of the phospholipids. The comparative study of the effects of these membrane-active antibiotics and the lipid bilayer structure demonstrated distinct types of antibiotic-membrane interactions in each case. Thus, the results showed the absence of interaction of polymyxin B with the dimyristoyllecithin membranes. In contrast, gramicidin S exhibited strong interaction with the lipid above the gel to liquid-crystalline phase transition temperature: disordering of the acyl side chains was evident. Increasing the concentration of gramicidin S led to disintegration of the bilayer membrane structure. At a molar ratio of 1:16 of gramicidin S to lecithin, the results are consistent with coexistence of gel and liquid-crystalline phases of the phospholipids near the phase transition temperature. Valinomycin decreased the phase transition temperature of the lipids and increased the order parameters of the lipid side chains. Such behavior is consistent with penetration of the valinomycin molecule into the interior of the lipid bilayers.
authors: Department of Biology, University of California, Riverside 92521.
authors: R
authors: R
authors: Zidovetzki
authors: U
authors: U
authors: Banerjee
authors: D W
authors: DW
authors: Harrington
authors: S I
authors: SI
authors: Chan
mesh_terms: D004134:Dimyristoylphosphatidylcholine; D006096:Gramicidin; D008051:Lipid Bilayers; D009682:Magnetic Resonance Spectroscopy; D011112:Polymyxin B; D011113:Polymyxins; D013696:Temperature; D014634:Valinomycin
chemical_list: D008051:Lipid Bilayers; D011113:Polymyxins; D006096:Gramicidin; D014634:Valinomycin; D011112:Polymyxin B; D004134:Dimyristoylphosphatidylcholine