Effect of monoclonal antihuman IgE on recombinant IgE(301-376) inhibition of specific IgE histamine release.
To explore the binding domains of rCIgE(301-376) necessary for inhibition of passive transfer of rye grass and Chinese elm IgE to human basophils, we employed monoclonal antibodies known to bind to IgE(301-336), M-272, and to IgE(367-376), M-27. By preincubating M-272, but not M-27, with rCIgE(301-376), passive transfer of specific IgE to basophils was partially inhibited. This implies that M-272 recognizes a binding site on rCIgE(301-376) or sterically interferes with the rCIgE(301-376) high-affinity binding domains on human basophils.
Glovsky
M M
MM
Department of Medicine, University of Southern California School of Medicine, Los Angeles.
Kebo
D
D
Helm
B A
BA
Horvath
S
S
Richards
J A
JA
Chretien
I
I
Bencherau
J
J
Gould
H
H
D000485:Allergens; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D000944:Antigens, Differentiation, B-Lymphocyte / Q000378:metabolism*; D001491:Basophils / Q000276:immunology*; D006636:Histamine Release; D006801:Humans; D007116:Immunization, Passive; D007073:Immunoglobulin E / Q000276:immunology*; D066298:In Vitro Techniques; D010446:Peptide Fragments; D011961:Receptors, Fc / Q000378:metabolism*; D017455:Receptors, IgE; D011994:Recombinant Proteins / Q000276:immunology; D013329:Structure-Activity Relationship; D014197:Trees
D000485:Allergens; D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000944:Antigens, Differentiation, B-Lymphocyte; D010446:Peptide Fragments; D011961:Receptors, Fc; D017455:Receptors, IgE; D011994:Recombinant Proteins; D007073:Immunoglobulin E