Lysis of CD3 hybridoma targets by cloned human CD4 lymphocytes.
UCHT1, a CD3-producing hybridoma, is lysed by cloned human T cells of both CD4 and CD8 phenotypes. The lysis is specific for the CD3 receptor and is mediated by 90% or more of all T cells from blood, without evidence for a subset of CD4 T cells incapable of cytotoxicity. CD4 clones characterized as 'helper' cells by in vitro help for specific antibody production by B cells also lysed CD3 targets. Varicella zoster virus (VZV) super-infected B lymphoblasts were lysed by three of six VZV-specific T-cell clones and 1 Cl-specific T-cell clones lysed 1 Cl hybridoma targets. Resting B cells (from tonsil) coated with CD3 antibody were lysed by CD4 clones. The data suggest that the majority of clonable CD4+ T cells lyse target cells recognized via CD3 or through the antigen receptor.
Hayward
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A
Human Tumour Immunology Group, University College London School of Medicine, U.K.
Boylston
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A
Beverley
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P
D000956:Antigens, Viral / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002999:Clone Cells; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000276:immunology; D014645:Herpesvirus 3, Human / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D008938:Mitosis; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
D000956:Antigens, Viral; D000939:Epitopes; D011948:Receptors, Antigen, T-Cell