Anterograde tracing in the brain using autoradiography and HRP-histochemistry. A comparison at the ultrastructural level.
The present paper describes the use of 3H-leucine and WGA-HRP as anterograde tracers in the brain at the ultrastructural level. In addition the different aspects of the two tracing techniques are compared, including the analysis of the results, the reliability and relative efficiency.
Holstege
J C
JC
Department of Anatomy, Erasmus University Medical School, Rotterdam, The Netherlands.
Vrensen
G F
GF
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D001921:Brain / Q000648:ultrastructure*; D001923:Brain Chemistry; D006651:Histocytochemistry / Q000379:methods*; D006735:Horseradish Peroxidase; D007930:Leucine / Q000032:analysis*; D008854:Microscopy, Electron; D014316:Tritium; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D014909:Wheat Germ Agglutinins
D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D014909:Wheat Germ Agglutinins; D014316:Tritium; D006735:Horseradish Peroxidase; D007930:Leucine