title: [Studies on monoclonal antibodies directed against human C-peptide].
abstract: By comparison, it was shown that ascaris protein as carrier was far more effective than BSA and mouse serum albumin to make human c-peptide (HCP) immunogenicity in mice. Twelve monoclonal antibodies (McAb) were obtained after fusion of NS-1 myeloma cells with spleen cells from two BALB/c mice immunized with HCP-ascaris protein conjugate. Eight McAb were characterized and all of them were of IgG1 subclass. Affinities of the McAb for HCP were in the range of 0.03-0.85 X 10(9) mol-1. Although all 8 McAb reacted with both HCP and human proinsulin (HPI), they could specifically recognize human insulin from HPI and were highly species specific without cross-reaction with proinsulin, insulin and c-peptide of other species tested. Through analysis of binding with different HCP fragments, their antigenic determinants were located at either N or C terminal of HCP. The results of plasma HCP radioimmunoassay in 20 normal fasting human adults showed that the value (0.47 +/- 0.16 pmol/ml) determined by rabbit PcAb (R2303) against BSA-HCP was significantly higher than that (0.29 +/- 0.13 pmol/ml) determined by McAb-1.
authors: C Y
authors: CY
authors: Wu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001200:Ascaris / Q000276:immunology; D002096:C-Peptide / Q000276:immunology*; D000939:Epitopes; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D011384:Proinsulin / Q000276:immunology; D011863:Radioimmunoassay
chemical_list: D000911:Antibodies, Monoclonal; D002096:C-Peptide; D000939:Epitopes; D011384:Proinsulin