title: Changes in cell shapes and cytokeratins of epithelial cells during the infiltration of lymphocytes in the human palatine tonsils.
abstract: By the immunohistochemical method using anti-keratin antibodies (KL1-antigen, 56 KD keratin; PKK1-antigen, 40-52.5 KD keratin) and electron microscopy, the changes in cell shapes and the cytokeratin components of the epithelial cells during the infiltration of lymphocytes were studied. In the surface epithelium, PKK1 reacts with only the keratinocytes in the basal layer, while KL1 stains in the spinous layer. In the neck portion of the crypt, transformed keratinocytes scattered in the spinous layer react intensely with PKK1. These cells issue prolonged cytoplasmic processes which surround the cavities filled with infiltrating lymphocytes. In the deep portion of the crypt, PKK1-positive cells interconnect with one another constituting the network of the star-shaped reticular cells. The enlarged intercellular spaces of the reticular cells are filled with many lymphocytes. Our observations suggest that the infiltration of lymphocytes into the crypt epithelium induces the change in keratin expression of the epithelial cells in the human palatine tonsils, and the change of keratin molecules makes the stratified flattened epithelium to transform into the network of star-shaped reticular cells.
authors: Department of Otolaryngology, Faculty of Medicine, Tokyo Medical and Dental University, Japan.
authors: Y
authors: Y
authors: Sato
authors: K
authors: K
authors: Wake
authors: I
authors: I
authors: Watanabe
mesh_terms: D002648:Child; D002675:Child, Preschool; D004848:Epithelium / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000378:metabolism*; D008214:Lymphocytes / Q000473:pathology*; D014066:Palatine Tonsil / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D014069:Tonsillitis / Q000378:metabolism / Q000473:pathology
chemical_list: D007633:Keratins