An immunisation protocol which enhances the frequency of antigen-specific monoclonal antibody production.
An immunisation protocol has been developed for small molecular weight antigens which results in a high percentage of specific hybridomas being produced after cell fusion. An enzyme-linked immunosorbent assay (ELISA) was developed for screening the desired antibodies in the culture supernatants. A conventional immunisation regimen was followed by doses of antigen in sterile water on each of the last 4 days before fusion. A range of antigen doses was used and the specific efficiency of fusion was increased by selection of the optimum amount. The antigen used as a model antigen in these experiments was biosynthetic human insulin.
Shibier
O
O
Department of Biochemistry, University of Surrey, Guildford, U.K.
Hampton
S M
SM
Marks
V
V
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D002459:Cell Fusion; D002460:Cell Line; D000939:Epitopes / Q000276:immunology*; D005260:Female; D006801:Humans; D007114:Immunization / Q000379:methods; D007115:Immunization Schedule; D007328:Insulin / Q000008:administration & dosage; D007330:Insulin Antibodies / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D010954:Plasmacytoma
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007328:Insulin; D007330:Insulin Antibodies