A new technique for staining catecholic residues in biological samples.
This technique for localizing catecholic residues in biological samples is based on the condensation of Besthorn's hydrazone (3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) with quinone residues obtained by the oxidation of catechols in the presence of ammonia. The product is a dark pink MBTH-quinone compound. This method is very sensitive and positive to catechol even at the 0.05 microgram level and the final product is chemically stable.
Kalyani
R
R
Department of Zoology, University of Madras, India.
Nellaiappan
K
K
D000818:Animals; D052160:Benzothiazoles; D002396:Catechols / Q000032:analysis*; D006652:Histological Techniques; D006835:Hydrazones; D008974:Mollusca; D010271:Parasites; D013194:Staining and Labeling; D013844:Thiazoles
D052160:Benzothiazoles; D002396:Catechols; D006835:Hydrazones; D013844:Thiazoles; C002536:3-methyl-2-benzothiazolone hydrazone