Enzyme-linked immunosorbent assay of basic pancreatic trypsin inhibitor.
Using specific rabbit antibody, one non-competitive (sandwich) and three variants of competitive enzyme-linked immunosorbent assays for basic pancreatic trypsin inhibitor (BPTI) were elaborated. For the assays peroxidase-antibody, two peroxidase-BPTI and beta-galactosidase-BPTI conjugates were applied. The best was competitive assay with peroxidase-BPTI conjugated by two step glutaraldehyde procedure. By this assay we were able to determine 1-10 ng BPTI/ml (0.007-0.07 KIU/ml) and the presence of human blood serum did not affect the assay of the inhibitor.
Szewczuk
A
A
Institue of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.
Rapak
A
A
Kurowska
E
E
Swidurska
E
E
D000818:Animals; D007611:Aprotinin / Q000032:analysis*; D002417:Cattle; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay
D007611:Aprotinin