Divalent cations modulate 5-HT3 receptor-induced currents in N1E-115 neuroblastoma cells.
The influence of extracellular calcium and magnesium ion concentrations upon 5-HT3 receptor-gated membrane currents in murine N1E-115 neuroblastoma cells has been studied under voltage-clamp conditions. A decrease in the concentration of either Ca2+ or Mg2+ from their standard values of 1.0 and 2.0 mM respectively augmented both the amplitude and duration of the 5-HT-induced current, whereas elevating the concentration of either divalent cation produced the opposite effect. Such modulation did not involve a change in the reversal potential of the response.
Peters
J A
JA
Department of Pharmacology and Clinical Pharmacology, Ninewells Hospital, University of Dundee, U.K.
Hales
T G
TG
Lambert
J J
JJ
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002412:Cations / Q000494:pharmacology*; D002478:Cells, Cultured; D007473:Ion Channels / Q000187:drug effects*; D008274:Magnesium / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism*; D009447:Neuroblastoma / Q000378:metabolism*; D011985:Receptors, Serotonin / Q000187:drug effects*
D002412:Cations; D007473:Ion Channels; D011985:Receptors, Serotonin; D008274:Magnesium; D002118:Calcium