Sensitive determination of deuterated and non-deuterated indole-3-acetic acid and 5-hydroxyindole-3-acetic acid by combined capillary gas chromatography-negative-ion chemical ionization mass spectrometry.
Sensitive methods for the determination of deuterated and non-deuterated indole-3-acetic acid and 5-hydroxyindole-3-acetic acid by combined capillary gas chromatography-negative-ion chemical ionization mass spectrometry were developed. Indole-3-acetic and 5-hydroxyindole-3-acetic acids were converted into pentafluorobenzyl and trifluoroacetylmethyl derivatives, respectively, after pre-purification by high-performance liquid chromatography. These derivatives were separated by gas chromatography and determined by selected ion monitoring. In the determinations, indole-3-acetic-2,2,2',4',5',6',7'-d7 acid and 5-hydroxyindole-3-acetic-3,3-d2 acid were used as internal standards. The methods developed in this work were used for the determination of deuterated and non-deuterated indole-3-acetic acid and 5-hydroxyindole-3-acetic acid in human urine samples collected before and after administration of L-tryptophan-3,3-d2.
Hayashi
T
T
National Centre of Neurology and Psychiatry, Tokyo, Japan.
Naruse
H
H
Matsuda
F
F
Iida
Y
Y
D000328:Adult; D002851:Chromatography, High Pressure Liquid; D003903:Deuterium; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000032:analysis* / Q000097:blood / Q000652:urine; D007202:Indicators and Reagents; D007210:Indoleacetic Acids / Q000032:analysis* / Q000097:blood / Q000652:urine; D008297:Male; D013056:Spectrophotometry, Ultraviolet
D007202:Indicators and Reagents; D007210:Indoleacetic Acids; D006897:Hydroxyindoleacetic Acid; C030737:indoleacetic acid; D003903:Deuterium