Monoclonal antibodies directed against human immunodeficiency virus (HIV) gag proteins with specificity for conserved epitopes in HIV-1, HIV-2 and simian immunodeficiency virus.
Monoclonal antibodies (MAbs) were raised against gag proteins of human immunodeficiency virus type 1 (HIV-1), strain HTLV-IIIB. One of 29 antibodies was specific for p17 of HIV-1. Twenty of 28 MAbs reactive with the major core protein p24 of HIV-1 showed cross-reactivity with HIV-2, and five of these also detected the corresponding antigens of simian immunodeficiency virus (SIVmac). The MAbs were reactive in several tests, i.e. ELISA, immunostaining of Western blots, immunofluorescence, alkaline phosphatase-anti-alkaline phosphatase immunocytochemistry and immunoelectron microscopy. The submembrane protein p17 was clearly localized within the virion.
Niedrig
M
M
Robert Koch-Institut des Bundesgesundheitsamtes, Berlin, F.R.G.
Rabanus
J P
JP
L'Age Stehr
J
J
Gelderblom
H R
HR
Pauli
G
G
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D015683:Gene Products, gag; D006678:HIV / Q000276:immunology*; D006801:Humans; D007118:Immunoassay; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D012190:Retroviridae / Q000276:immunology*; D012191:Retroviridae Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes; D015683:Gene Products, gag; D012191:Retroviridae Proteins