An antigenic peptide of the HIV-1 NEF protein recognized by cytotoxic T lymphocytes of seropositive individuals in association with different HLA-B molecules.
By using target cells that expressed only the regulatory NEF protein of the human immunodeficiency virus (HIV), we were able to identify specific cytolytic T cells (CTL) from most of the seropositive patients tested. This NEF-specific cytolytic activity was mediated by CD8+ lymphocytes. In two different donors, we identified a unique peptide in the NEF protein that could be recognized in association with two different HLA class I molecules. The definition of such a peptide should prove useful in evaluating vaccine strategies.
Culmann
B
B
INSERM U 152-CNRS 628, Hôpital Cochin, Paris, France.
Gomard
E
E
Kiény
M P
MP
Guy
B
B
Dreyfus
F
F
Saimot
A G
AG
Sereni
D
D
Lévy
J P
JP
D000595:Amino Acid Sequence; D000939:Epitopes; D015693:Gene Products, nef / Q000276:immunology*; D015488:HIV Antigens / Q000276:immunology*; D006679:HIV Seropositivity / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D015235:HLA-B Antigens / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D054334:Viral Regulatory and Accessory Proteins / Q000276:immunology*; D054311:nef Gene Products, Human Immunodeficiency Virus
D000939:Epitopes; D015693:Gene Products, nef; D015488:HIV Antigens; D015235:HLA-B Antigens; D010446:Peptide Fragments; D054334:Viral Regulatory and Accessory Proteins; D054311:nef Gene Products, Human Immunodeficiency Virus