Site-directed enzyme-linked immunosorbent assay with a synthetic simian immunodeficiency virus SIVmac peptide identifying antibodies against the HIV-2 transmembrane glycoprotein.
A 23-amino-acid-long peptide (AIEKYLEDQAQLNAWGCAFRQVC) representing the transmembranous protein gp32 in SIVmac was used in site-directed enzyme-linked immunosorbent assay (ELISA) for detection of HIV-2-specific antibodies in 567 sera from Bissau, Guinea Bissau. Ninety out of the 567 sera were identified to contain HIV-2 antibodies by whole antigen ELISA and Western blot assays. The peptide ELISA correctly identified 89 out of these 90 seropositives (sensitivity 98.9%). Three sera falsely interpreted to be positive were encountered (specificity 99.4%). The HIV-2 peptide was also used for testing of 93 HIV-1-positive Swedish sera. None of these sera reacted. Site-directed serology employing synthetic peptides should be considered for application as a screening assay.
Norrby
E
E
Department of Virology, Karolinska Institutet, School of Medicine, Stockholm, Sweden.
Chiodi
F
F
Whalley
A
A
Parks
E
E
Nauclér
A
A
Costa
C M
CM
Torstensson
R
R
Biberfeld
G
G
D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D005189:False Positive Reactions; D015686:Gene Products, env; D006169:Guinea-Bissau; D015483:HIV Antibodies / Q000032:analysis*; D015497:HIV-1 / Q000276:immunology; D015498:HIV-2 / Q000276:immunology*; D006801:Humans; D010446:Peptide Fragments / Q000276:immunology*; D011237:Predictive Value of Tests; D012191:Retroviridae Proteins / Q000276:immunology*; D015682:Retroviridae Proteins, Oncogenic; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*; D014760:Viral Fusion Proteins
D000939:Epitopes; D015686:Gene Products, env; D015483:HIV Antibodies; D010446:Peptide Fragments; D012191:Retroviridae Proteins; D015682:Retroviridae Proteins, Oncogenic; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; C059580:transmembrane protein, Simian immunodeficiency virus