Selected-ion monitoring gas chromatographic-mass spectrometric analysis of catecholamines: enhancement of sensitivity by a simple clean-up step on Sephadex G-10.
The sensitivity of catecholamines using selected-ion monitoring gas chromatography-mass spectrometry (SIM GC-MS) has been enhanced by employing a simple clean-up step using Sephadex G-10 columns. This procedure allows for the detection of extremely small amounts (1-10 pg) of biogenic amines (e.g., epinine) which corresponds to a 25-fold increase in detection limits compared to electron ionization GC-MS or high-performance liquid chromatography with electrochemical detection (HPLC-ED). The SIM GC-MS assay was used to monitor the dopamine-beta-hydroxylase-mediated conversion of epinine d6 to epinephrine d6 in rat hypothalamus and brainstem in vitro and the results were compared to those obtained by HPLC-ED.
Scriba
G K
GK
Department of Pharmaceutical Chemistry, University of Kansas, Lawrence 66045.
Borchardt
R T
RT
Zirrolli
J A
JA
Fennessey
P V
PV
D000818:Animals; D001923:Brain Chemistry; D002395:Catecholamines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003911:Dextrans; D004563:Electrochemistry; D008401:Gas Chromatography-Mass Spectrometry; D005782:Gels; D051381:Rats
D002395:Catecholamines; D003911:Dextrans; D005782:Gels; C025614:sephadex