[Morphometric research on the nuclei and argentophilic structures of the nucleoli of granulocytopoietic cells in rats after long-term cyclophosphamide exposure].
The Ag-staining intensity of interphase bone marrow granulopoietic cells decreases during their maturation as evidenced by TV computerized morphometry. These data are in accordance with the phenomenon of RNA transcription reduction in mature granulocytes. The Ag-positive material amount increases during S and G2 phases of proliferative pool granulocytes. 8 days after every day injection of 20 mg/kg cyclophosphamide an integral area of silver grains in proliferative pool granulocytes decreased 1.5 times. In mature granulocytes it decreased and was two times higher than in the control. The nuclear area of all granulocytes increased. There was a positive correlation (p less than 0.01) between the quantity of the silver grains and nuclear areas. It seems that mature granulocytes inherit the increased Ag-positive material amount from G2 blocked progenitors (promyelocytes and myelocytes) and this increase is not due to the RNA transcriptional activity.
Pogorelov
V M
VM
Berger
J J
JJ
Kozinets
G I
GI
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000648:ultrastructure; D002466:Cell Nucleolus / Q000187:drug effects* / Q000648:ultrastructure; D002467:Cell Nucleus / Q000187:drug effects* / Q000648:ultrastructure; D003520:Cyclophosphamide / Q000494:pharmacology*; D006098:Granulocytes / Q000187:drug effects* / Q000648:ultrastructure; D006410:Hematopoiesis / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors
D003520:Cyclophosphamide
Morfometricheskoe issledovanie iader i argentofil'nykh struktur nukleol kletok granulotsitopoéza krys posle dlitel'nogo vozdeĭstviia tsiklofosfamida.