[Regulation of proteolytic processes using antisense RNA genes htpR and lon of Escherichia coli in hetero- and homologous genetic systems].
Possibility of correction of proteolytic processes in cells of Escherichia coli and Pseudomonas aeruginosa has been studied. For this purpose recombinant plasmids directing the synthesis of antisense RNAs were constructed. In Ps. aeruginosa the synthesis of htpR antisense RNA resulted in 2.5-fold reduction of the intensity of degradation of 3H-puromycin polypeptides under heat shock conditions. An antisense RNA complementary to the 5'- end of E. coli lon gene decreased the same index to the level observed in lon- mutants. Genes homologous to htpR and lon genes of E. coli were found in Pseudomonas: bacteria in hybridisation experiments. This finding suggests that the genetic system of heat shock in these microorganisms is organized in a similar manner.
Kiselev
V I
VI
Chistiakova
L G
LG
Antonov
V K
VK
D001345:Autoradiography; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D006868:Hydrolysis; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000235:genetics*; D016376:Oligonucleotides, Antisense; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense; D012329:RNA, Bacterial
Reguliatsiia proteoliticheskikh protsessov s pomoshch'iu antismyslovykh RNK genov htpR i lon Escherichia coli v getero- i gomologichnykh genetichestkikh sistemakh.