Purification of GTP cyclohydrolase I from human liver and production of specific monoclonal antibodies.
GTP cyclohydrolase I, the first enzyme in the de novo biosynthesis of tetrahydrobiopterin, was enriched more than 13,000-fold from human liver by preparative isoelectric focusing using Sephadex G-200 SF gels. The pI of the active enzyme was determined as 5.6 by analytical isoelectric focusing in the same matrix. The native enzyme has an apparent molecular mass of 440 kDa and appears to be composed of eight 50-kDa subunits as estimated from SDS/PAGE. The enriched enzyme preparation was used to produce specific monoclonal antibodies. From 11 monoclonal antibodies obtained, one was extensively characterized for further applications. This monoclonal antibody belongs to the IgM class and shows immunoreactivity with GTP cyclohydrolase I both from man and from Escherichia coli. It is capable of highly sensitive detection of GTP cyclohydrolase I by ELISA and by Western blot analysis. The monoclonal antibody was used for the immunoenzymatic localisation of GTP cyclohydrolase I in human peripheral blood mononuclear cells. Furthermore, it was possible to demonstrate the absence of immunoreactivity in cells with GTP cyclohydrolase I deficiency. The antibody's use as a tool either for differential diagnosis of atypical phenylketonuria due to GTP cyclohydrolase I deficiency or prenatal diagnosis of this severe inherited metabolic disease is now under investigation.
Schoedon
G
G
Department of Pediatrics, University of Zurich, Switzerland.
Redweik
U
U
Curtius
H C
HC
D000619:Aminohydrolases / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal; D002850:Chromatography, Gel / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D006136:GTP Cyclohydrolase / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D007202:Indicators and Reagents; D007525:Isoelectric Focusing / Q000379:methods; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007202:Indicators and Reagents; D000619:Aminohydrolases; D006136:GTP Cyclohydrolase