Cloning, characterization, and expression of a human insulin-like growth factor binding protein.
Insulin-like growth factors (IGFs) bind to specific proteins present in extracellular fluids. One of these binding proteins (IGF-BP) was purified from human amniotic fluid and was shown to potentiate the effects of IGF-I in vitro (10). In these studies, a polyclonal antibody to this protein was used to isolate a cDNA clone from a human decidua library. This clone encodes a polypeptide of 25,832 daltons that includes the sequences of 9 tryptic peptides that had been prepared from the purified IGF-BP. The protein has 15 cysteines that are clustered at the amino and carboxy ends of the molecule. The protein has an RGD sequence near its C-terminus, which may account for its ability to attach to cells and to potentiate the biological actions of IGF-I.
Brewer
M T
MT
Synergen, Inc., Boulder, CO 80301.
Stetler
G L
GL
Squires
C H
CH
Thompson
R C
RC
Busby
W H
WH
Clemmons
D R
DR
D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D018969:Insulin-Like Growth Factor Binding Proteins; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010957:Plasmids
D002352:Carrier Proteins; D018969:Insulin-Like Growth Factor Binding Proteins