title: Gold labeling with wheat germ agglutinin and RNAse on osmicated tissue embedded in epoxy resin.
abstract: Tissue of an insect, Lucilia cuprina, fixed conventionally in buffered glutaraldehyde and osmium and embedded in epoxy resin (epon or epon/araldite), provided sections which could readily be labeled with RNAse/gold and wheat germ agglutinin (WGA)/gold. This method offers labeling of tissues with improved contrast and allows the retrospective application of RNAse and WGA labeling to conventionally prepared tissues, without recourse to oxidizing/etching agents.
authors: CSIRO, Division of Entomology, Canberra, Australia.
authors: E M
authors: EM
authors: Brooks
authors: K C
authors: KC
authors: Binnington
mesh_terms: D000818:Animals; D002686:Chitin / Q000032:analysis*; D003102:Colloids; D004175:Diptera / Q000032:analysis; D004853:Epoxy Resins; D006046:Gold; D006651:Histocytochemistry / Q000379:methods*; D009993:Osmium Tetroxide; D012313:RNA / Q000032:analysis*; D012260:Ribonucleases; D014909:Wheat Germ Agglutinins
chemical_list: D003102:Colloids; D004853:Epoxy Resins; D014909:Wheat Germ Agglutinins; D002686:Chitin; D012313:RNA; D006046:Gold; D012260:Ribonucleases; D009993:Osmium Tetroxide