title: Immunoglobulins in bile and serum of the rabbit associated with protection after Vibrio cholerae infection and vaccination.
abstract: Previous studies have shown that cholera, as well as protective immunity against infection with Vibrio cholerae, can be induced in the rabbit. This protection is long-lasting (up to 30 months) and is characterized on challenge by rapid, symptom-free disappearance of V. cholerae from the intestine; we therefore believe this to be vibriocidal protection. In this study, we analysed the humoral and secretory immune response against various subcellular V. cholerae components in vibriocidally protected, non-vibriocidally protected, and unprotected animals. Only vibriocidal protection was found to be associated with high levels of biliary IgA directed against lipopolysaccharide O antigen. We did not find such a correlation between either type of protection and response in serum. Therefore, anti-lipopolysaccharide antibodies are essential in protection against experimental infection with V. cholerae.
authors: National Institute of Public Health and Environmental Hygiene, Bilthoven, The Netherlands.
authors: S G
authors: SG
authors: Rijpkema
authors: W H
authors: WH
authors: Jansen
authors: H
authors: H
authors: Gielen
authors: P A
authors: PA
authors: Guinée
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001646:Bile / Q000276:immunology*; D002771:Cholera / Q000276:immunology* / Q000517:prevention & control; D002772:Cholera Toxin / Q000276:immunology; D022121:Cholera Vaccines / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D007071:Immunoglobulin A, Secretory / Q000096:biosynthesis; D007136:Immunoglobulins / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000276:immunology; D019081:O Antigens; D011817:Rabbits; D012463:Saliva / Q000276:immunology; D014611:Vaccination; D014734:Vibrio cholerae / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D022121:Cholera Vaccines; D007071:Immunoglobulin A, Secretory; D007136:Immunoglobulins; D008070:Lipopolysaccharides; D019081:O Antigens; D002772:Cholera Toxin