A new species of multicopy single-stranded DNA from Myxococcus xanthus with conserved structural features.
Myxobacteria have been shown to contain a large number of branched RNA-linked single-stranded DNA (multicopy single-stranded DNA (msDNA] molecules. In addition, we found that Myxococcus xanthus contains another smaller msDNA-like molecule, designated mrDNA, consisting of a 65-base single-stranded DNA covalently linked by a 2',5'-phosphodiester linkage to a 49-base branched RNA. In spite of their different primary sequences, the RNA-linked mrDNA is remarkably similar in secondary structure to msDNA, sharing similar stem-loop folding as well as the unique 2',5'-phosphodiester linkage. These results indicate that these novel molecules are synthesized by common molecular mechanisms.
Dhundale
A
A
Department of Biochemistry, Robert Wood Johnson Medical School, Rutgers University of Medicine and Dentistry, Piscataway, New Jersey 08854.
Inouye
M
M
Inouye
S
S
D001483:Base Sequence; D004277:DNA, Single-Stranded / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009231:Myxococcales / Q000235:genetics*; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial / Q000235:genetics
D004277:DNA, Single-Stranded; D012329:RNA, Bacterial