Isolation and some properties of mammalian hepatic membrane lectins.
Membrane lectins were isolated from sheep, goat, and buffalo liver by chromatography on an asialofetuin (ASF)-Sepharose 4B column. The lectins moved as a single protein band in SDS-PAGE with molecular masses of 42, 54 and 50 kDa, respectively, for sheep, goat and buffalo lectins. The molecular masses remained unchanged in 0.2 M 2-mercaptoethanol. As judged from the inhibition of binding of the lectin to ASF gel, the three lectins were beta-galactoside-specific. Sheep, goat and buffalo lectins were found to be sialoglycoproteins containing 18.6, 27 and 38.8 mol/mol lectin of neutral hexose, respectively; the corresponding values for the sialic acid content being 5.3, 8.7 and 11.8 mol/mol lectin. Thus goat and buffalo lectins are physico-chemically different from many mammalian hepatic lectins described so far.
Ali
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Interdisciplinary Biotechnology Unit, Aligarh Muslim University, India.
Salahuddin
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A
D000818:Animals; D037263:Asialoglycoprotein Receptor; D001212:Asialoglycoproteins; D002020:Buffaloes / Q000378:metabolism*; D002462:Cell Membrane / Q000032:analysis*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D060748:Fetuins; D005697:Galactosides / Q000378:metabolism; D006041:Goats / Q000378:metabolism*; D006601:Hexoses / Q000032:analysis; D006801:Humans; D008099:Liver / Q000032:analysis*; D008970:Molecular Weight; D019158:N-Acetylneuraminic Acid; D011971:Receptors, Immunologic / Q000302:isolation & purification* / Q000378:metabolism; D012756:Sheep / Q000378:metabolism*; D012794:Sialic Acids / Q000032:analysis; D000509:alpha-Fetoproteins
D037263:Asialoglycoprotein Receptor; D001212:Asialoglycoproteins; D060748:Fetuins; D005697:Galactosides; D006601:Hexoses; D011971:Receptors, Immunologic; D012794:Sialic Acids; D000509:alpha-Fetoproteins; C017029:asialofetuin; C007816:beta-galactoside; D019158:N-Acetylneuraminic Acid