The genes for the frog skin peptides GLa, xenopsin, levitide and caerulein contain a homologous export exon encoding a signal sequence and part of an amphiphilic peptide.
From genomic libraries of Xenopus laevis, parts of the genes coding for the precursors of the skin peptides GLa (peptide with amino-terminal glycine and carboxy-terminal leucinamide), xenopsin and levitide have been isolated and sequenced. The gene for prepropeptide GLa comprises four exons, separated by relatively small introns. The gene for preproxenopsin is composed of five exons, of which all but the last one have been analyzed. This is a large gene encompassing at least 25,000 base pairs. In addition, two exons of the gene for preprolevitide have been isolated. A comparison of these genes reveals the presence of a homologous exon. This exon contains 161 bp, starts one base pair prior to the initiation codon and encodes a signal peptide and part of a pro region with processing sites. In addition, the two genes for preprocaerulein analyzed previously [Vlasak et al. (1987) Eur. J. Biochem. 169, 53-58] also contain a similar exon. This demonstrates the existence of a homologous export exon in genes encoding the precursors of different skin peptides.
Kuchler
K
K
Institute of Molecular Biology, Austrian Academy of Sciences, Salzburg, Austria.
Kreil
G
G
Sures
I
I
D000818:Animals; D023181:Antimicrobial Cationic Peptides; D001483:Base Sequence; D002108:Ceruletide / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D005091:Exons; D005796:Genes; D005815:Genetic Code; D007438:Introns; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000235:genetics; D010455:Peptides / Q000032:analysis / Q000235:genetics*; D021382:Protein Sorting Signals / Q000235:genetics*; D012313:RNA / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid; D012867:Skin / Q000032:analysis*; D029867:Xenopus Proteins; D014982:Xenopus laevis
D023181:Antimicrobial Cationic Peptides; D009842:Oligopeptides; D010455:Peptides; D021382:Protein Sorting Signals; D029867:Xenopus Proteins; C045866:peptide-Gly-Leu-amide; C055061:levitide; C016832:xenopsin; D012313:RNA; D002108:Ceruletide; D004247:DNA