Release and synthesis of histamine in rat basophilic leukemia (2H3) cells.
On the stimulation of rat basophilic leukemia (2H3) cells with an IgE oligomer, the intracellular Ca2+ concentration, which was measured with fura-2 as a fluorescent probe, began to increase after 1 min lag period, reached its maximum at 4 min, and gradually decreased to the original level. The histamine release occurred slightly more slowly than the increase in the intracellular Ca2+. It started 2-3 min after the stimulation, reaching a peak at 6 min and thereafter decreasing slowly. To supplement the released histamine, the activity of histidine decarboxylase, a histamine-forming enzyme, increased 5-fold several hours after the stimulation by an IgE oligomer, and this increase may be mediated by protein kinase C system.
Watanabe
T
T
Department of Pharmacology I, Tohoku University School of Medicine, Sendai, Japan.
Maeyama
K
K
Taguchi
Y
Y
D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005456:Fluorescent Dyes; D006632:Histamine / Q000096:biosynthesis*; D006636:Histamine Release; D006640:Histidine Decarboxylase / Q000378:metabolism; D007073:Immunoglobulin E / Q000276:immunology; D007942:Leukemia, Experimental / Q000276:immunology* / Q000378:metabolism; D051381:Rats; D013997:Time Factors
D005456:Fluorescent Dyes; D007073:Immunoglobulin E; D006632:Histamine; D006640:Histidine Decarboxylase