[Experience with the use of a commercial immunoglobulin diagnostic agent for determining HBsAg in the reverse passive hemagglutination reaction under the conditions of Central Asia].
Use of twice-distilled water or addition of normal equine serum, preheated at 56 degrees C for 30 min and adsorbed with an adsorbent from the diagnostic kit, to normal saline (1 ml of serum per 100 ml of the saline) helps eliminate spontaneous agglutination of the tested diagnosticum during the detection of HBsAg with the use of reverse passive hemagglutination test, when the experiments are carried out in areas with high concentrations of salts and organic substances in the water.
Makarova
G I
GI
Alekseenkova
L P
LP
D001209:Asia, Central; D006386:Hemagglutination Tests / Q000379:methods; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006801:Humans; D011933:Reagent Kits, Diagnostic; D014881:Water Supply
D006514:Hepatitis B Surface Antigens; D011933:Reagent Kits, Diagnostic
Opyt primeneniia kommercheskogo immunoglobulinovogo diagnostikuma dlia opredeleniia HBsAg v reaktsii obratnoĭ passivnoĭ gemaggliutinatsii v usloviiakh Sredneĭ Azii.