Effects of microgravity on liposome-reconstituted cardiac gap junction channeling activity.
Effects of microgravity on cardiac gap junction channeling activity were investigated aboard NASA zero-gravity aircraft. Liposome-reconstituted gap junctions were assayed for channel function during free-fall, and the data were compared with channeling at 1 g. Control experiments tested for 0 g effects on the structural stability of liposomes, and on the enzyme-substrate signalling system of the assay. The results demonstrate that short periods of microgravity do not perturb reconstituted cardiac gap junction channeling activity.
Claassen
D E
DE
BioServe Space Technologies, NASA, Manhattan, KS 66506.
Spooner
B S
BS
D000818:Animals; D002645:Chickens; D017630:Connexins; D006112:Gravitation; D006321:Heart / Q000502:physiology*; D007365:Intercellular Junctions / Q000201:enzymology / Q000502:physiology*; D007473:Ion Channels / Q000201:enzymology / Q000502:physiology*; D008081:Liposomes; D008565:Membrane Proteins / Q000502:physiology; D008954:Models, Biological; D010544:Peroxidases / Q000378:metabolism; D013379:Substrate Specificity
D017630:Connexins; D007473:Ion Channels; D008081:Liposomes; D008565:Membrane Proteins; D010544:Peroxidases; C021799:microperoxidase