Maintenance of the Drosophila melanogaster genomic fragment harbouring the mdg4 copia-like retrotransposon in tobacco cells.
Recent experiments have revealed that plant genomes contain genetic elements closely related to well-known yeast and Drosophila retrotransposons. In connection with these findings we decided to test the behaviour of one of the well-defined Drosophila retrotransposons in tobacco cells. The copia-like Drosophila melanogaster retrotransposon mdg4 was introduced into Nicotiana tabacum and Nicotiana plumbaginifolia cells using a Ti plasmid vector. The results showed that entire copies of mdg4, as part of pTiC58 T-DNA, were stably maintained in tobacco cells upon continuous culturing of transformed tumour lines on hormone-free medium. Transcription of mdg4 from its LTR promoter was not detectable in tobacco cells.
Brzobohatý
B
B
Institute of Biophysics, Czechoslovak Academy of Sciences, Brno.
Karlovská
L
L
Vyskot
B
B
Bezdĕk
M
M
D000818:Animals; D002460:Cell Line; D004247:DNA / Q000032:analysis / Q000235:genetics; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics*; D010947:Plants, Toxic; D010957:Plasmids; D012313:RNA / Q000302:isolation & purification; D014026:Nicotiana / Q000235:genetics*
D004251:DNA Transposable Elements; D012313:RNA; D004247:DNA