Different preparations of natural and recombinant human interleukin-6 (IFN-beta 2, BSF-2) similarly stimulate acute phase protein synthesis and uptake of alpha-aminoisobutyric acid by cultured rat hepatocytes.
1. Rat hepatocytes were cultured for 2 days in Williams E medium containing 1 microM insulin and dexamethasone. 2. Production of five plasma proteins was determined by electroimmunoassay in the media, and amino acid uptake was measured by [alpha-14C]aminoisobutyric acid accumulation in hepatocytes. 3. Supernatants from rat peritoneal macrophages and IL-6/IFN-beta 2/BSF-2 obtained from four different laboratories similarly stimulated synthesis of fibrinogen, alpha 1-cysteine proteinase inhibitor and alpha 2-macroglobulin, as well as [14C]-accumulation in cultured hepatocytes. 4. It is concluded that IL-6 is the principal hepatocyte stimulating factor responsible for typical features of the acute phase response of liver cells.
Bereta
J
J
Institute of Molecular Biology, Jagiellonian University, Krakow, Poland.
Kurdowska
A
A
Koj
A
A
Hirano
T
T
Kishimoto
T
T
Content
J
J
Fiers
W
W
Van Damme
J
J
Gauldie
J
J
D000209:Acute-Phase Proteins / Q000096:biosynthesis*; D000621:Aminoisobutyric Acids / Q000493:pharmacokinetics*; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D015850:Interleukin-6; D007378:Interleukins / Q000302:isolation & purification / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology
D000209:Acute-Phase Proteins; D000621:Aminoisobutyric Acids; D015850:Interleukin-6; D007378:Interleukins; D011994:Recombinant Proteins; C100049:2-aminoisobutyric acid