Simplified C-banding of mouse chromosomes.
Mouse bone marrow chromosomes incorporated with bromodeoxyuridine (BrdU) were differentially Giemsa stained after exposure to incandescent light in order to study sister chromatid exchange. Over-exposure of the chromosomes to incandescent light often resulted in the complete loss of sister chromatid differentiation, and it frequently resulted in the C-band formation. C-banding was also prominent with chromosomes which were not labelled with BrdU. The method utilized a 300 W, 130 V, inside-frosted incandescent bulb coupled with phosphate buffering at pH 6.8 and an exposure period of 2 h.
Edelman
J R
JR
Department of Biological Sciences, St John's University, Jamaica, New York 11439.
Lin
Y J
YJ
D000818:Animals; D001853:Bone Marrow / Q000032:analysis / Q000378:metabolism; D001854:Bone Marrow Cells; D001973:Bromodeoxyuridine / Q000378:metabolism / Q000494:pharmacology; D002871:Chromosome Banding / Q000379:methods*; D002875:Chromosomes / Q000648:ultrastructure*; D008027:Light; D008677:Metaphase; D051379:Mice; D012854:Sister Chromatid Exchange / Q000187:drug effects; D013194:Staining and Labeling / Q000379:methods
D001973:Bromodeoxyuridine