Pharmacokinetics of 125I-hirudin in rats and dogs.
Hirudin was 125I-labelled using a modified chloramine-T method. 125I-hirudin proved to be a suitable marker in pharmacokinetic studies, if unchanged 125I-hirudin in body fluids was determined by means of a binding assay using immobilized thrombin. In rats and dogs a study was performed on the pharmacokinetic behaviour of hirudin following intravenous and subcutaneous injection, resp., or one-hour infusion and pharmacokinetic parameters were calculated.
Richter
M
M
Institute of Pharmacology and Toxicology, Medical Academy, Erfurt, GDR.
Cyranka
U
U
Nowak
G
G
Walsmann
P
P
D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000652:urine; D004285:Dogs; D005260:Female; D006629:Hirudins / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D007457:Iodine Radioisotopes; D008297:Male; D011869:Radioligand Assay; D051381:Rats; D011919:Rats, Inbred Strains; D013917:Thrombin
D015415:Biomarkers; D006629:Hirudins; D007457:Iodine Radioisotopes; D013917:Thrombin