Identification of a cDNA coding for the SerH3 surface protein of Tetrahymena thermophila.
We have identified a Tetrahymena thermophila cDNA-containing plasmid (pC6) which hybridizes to a 1.47-kB RNA whose changes in cellular concentration parallel the changes in synthetic rate of a major cell surface protein. From a molecular and genetic analysis of strains expressing the gene (SerH3) encoding this protein, and of strains expressing immunologically distinct alleles of this gene, we conclude that pC6 encodes a portion of the SerH3 allele.
Doerder
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FP
Department of Biology, Cleveland State University, Ohio 44115.
Hallberg
R L
RL
D000818:Animals; D000953:Antigens, Protozoan; D000954:Antigens, Surface / Q000096:biosynthesis / Q000235:genetics*; D003433:Crosses, Genetic; D004247:DNA / Q000302:isolation & purification*; D015335:Molecular Probes; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D015800:Protozoan Proteins; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D013045:Species Specificity; D013696:Temperature; D013768:Tetrahymena / Q000235:genetics* / Q000276:immunology
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D015335:Molecular Probes; D015800:Protozoan Proteins; C043323:immobilization antigens; D012313:RNA; D004247:DNA