The influence of Romanowsky-Giemsa type of stains on the nuclear texture of desoxyribonuclease-treated cytological preparations.
It had been observed that cell films where DNA had been partially digested by DNase stained differentially by the standard RG stain and a representative commercial Giemsa mixture. The reasons for this divergence were experimentally investigated. The lower concentration of Azure B in the commercial Giemsa mixture as compared with the standard RG stain emerged as the most likely factor causing the divergence of staining behaviour. A correlation of this assumption to the theory of the RG stain is tentatively suggested.
Wittekind
D
D
Anatomisches Institut II der Universität Freiburg.
Schulte
E
E
Kretschmer
V
V
D000818:Animals; D001202:Ascitic Fluid / Q000473:pathology*; D001399:Azure Stains; D001853:Bone Marrow / Q000473:pathology*; D002467:Cell Nucleus / Q000648:ultrastructure*; D002843:Chromatin / Q000648:ultrastructure; D003851:Deoxyribonucleases; D004801:Eosine Yellowish-(YS); D005260:Female; D006168:Guinea Pigs; D006801:Humans; D010051:Ovarian Neoplasms / Q000473:pathology*; D010053:Ovary / Q000473:pathology; D010640:Phenothiazines
D001399:Azure Stains; D002843:Chromatin; D010640:Phenothiazines; C046541:Romanowsky-Giemsa stain; D003851:Deoxyribonucleases; D004801:Eosine Yellowish-(YS)