Human monocyte activation induced by an anti-CD14 monoclonal antibody.
An anti-CD14 mAb RoMo-1 rapidly induces in human monocytes a transient oxidative burst activity as detected by chemiluminescence assay. Pretreatment of these cells with the mAb markedly suppresses the monocyte chemiluminescence response to opsonized zymosan. In addition, the antibody induces a significant increase of IL-1 production and secretion by mononuclear cells, comparable to a similar effect of rIFN-gamma or LPS. Electron microscopy demonstrates internalization of the CD14 molecules after interaction with the mAb in a characteristic receptor-like manner.
Schütt
C
C
Department of Medical Immunology, Ernst-Moritz-Arndt-University, Greifswald, G.D.R.
Ringel
B
B
Nausch
M
M
Bazil
V
V
Horejsí
V
V
Neels
P
P
Walzel
H
H
Jonas
L
L
Siegl
E
E
Friemel
H
H
D000911:Antibodies, Monoclonal; D015214:Antigens, Differentiation, Myelomonocytic; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000096:biosynthesis; D018950:Lipopolysaccharide Receptors; D008163:Luminescent Measurements; D008854:Microscopy, Electron; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism / Q000648:ultrastructure; D015054:Zymosan / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D015214:Antigens, Differentiation, Myelomonocytic; D007375:Interleukin-1; D018950:Lipopolysaccharide Receptors; D015054:Zymosan