Antitumor effect of a baker's yeast mannan-mitomycin C conjugate against mouse hepatoma, MH134, in vivo and in vitro.
A conjugate of mitomycin C and the mannan of bakers' yeast (Saccharomyces cerevisiae wild type strain) was synthesized. Assay of its growth inhibitory effect on MH134 hepatoma solid tumor implanted in C3H/He slc mice showed that this conjugate exhibited a higher growth-inhibition ratio than those of free mitomycin C and the same bakers' yeast mannan in the corresponding effective doses. This conjugate was also found to kill the same hepatoma cells in vitro. Because a dextran-mitomycin C conjugate did not manifest any antitumor effect against this tumor, it was postulated that interaction of the mannan moiety in the mannan-mitomycin C conjugate with the mannose receptor located on the cell surface of immunocytes and/or with hepatoma cells participated in the common initiating reaction of in vitro and in vivo antitumor effects.
Aizawa
K
K
Department of Microbiology, Tohoku College of Pharmacy, Miyagi, Japan.
Matsumoto
T
T
Tsukada
K
K
Ito
A
A
Sato
H
H
Suzuki
S
S
Suzuki
M
M
D000818:Animals; D003911:Dextrans / Q000008:administration & dosage; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy*; D008297:Male; D008351:Mannans / Q000008:administration & dosage*; D051379:Mice; D008809:Mice, Inbred C3H; D016685:Mitomycin; D008937:Mitomycins / Q000008:administration & dosage*; D012441:Saccharomyces cerevisiae; D014407:Tumor Cells, Cultured / Q000187:drug effects
D003911:Dextrans; D008351:Mannans; D008937:Mitomycins; D016685:Mitomycin