title: Monoclonal antibody-detected vimentin distribution in pleomorphic adenomas of salivary glands.
abstract: To evaluate the participation of myoepithelial components in pleomorphic adenomas, an immunohistochemical study was carried out using monoclonal antibodies to vimentin. Of a total of 80 cases, 50 tumors gave positive staining, 5 tumors very slight, and 25 tumors negative staining for vimentin. Localization patterns for vimentin were divided into 3 classes: 1) vimentin staining in fibrous stromal tissue; 2) variable intensities of vimentin staining were found in the outer layers of tumor cells in tubulo-ductal structures (some of which were spindle cells connected to modified myoepithelial cells which also gave variable vimentin staining); and 3) modified myoepithelial cells and chondroidlike cells displayed strongly positive staining for vimentin. Typical histologic features of pleomorphic ademomas, i.e., tubulo-ductal or duct-like structures were characterized by positive vimentin staining in outer tumor cells and by a positive keratin reaction in the luminal tumor cells. In tumors devoid of stromal connective tissues and the near absence of well-developed, or modified myoepithelial cells, vimentin staining was absent.
authors: Department of Oral Surgery, Asahi University School of Dentistry, Gifu, Japan.
authors: K
authors: K
authors: Yamada
authors: H
authors: H
authors: Shinohara
authors: Y
authors: Y
authors: Takai
authors: M
authors: M
authors: Mori
mesh_terms: D008949:Adenoma, Pleomorphic / Q000473:pathology*; D000911:Antibodies, Monoclonal; D004848:Epithelium / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D012468:Salivary Gland Neoplasms / Q000473:pathology*; D012469:Salivary Glands / Q000166:cytology; D013194:Staining and Labeling; D014746:Vimentin / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D014746:Vimentin