[Reduction of actinomycin biosynthesis during protoplast regeneration in an inactive variant producer].
During regeneration of protoplasts in the inactive variant H-2 of the actinomycin-producing organism Streptomyces sp. 26-115 there were detected 1-4 per cent of the colonies synthesizing the antibiotic. The frequency of such colonies (H-2R) did not increase after exposure of the H-2 protoplasts to the fusing agent PEG-1000. The population grown from one colony after three passages on pea agar was sufficiently homogeneous by the antibiotic production property. Variant H-2R was more stable to the effect of streptomycin than the initial variant H-2.
Orlova
T I
TI
Volkova
E I
EI
Kuroedov
A A
AA
D001431:Bacteriological Techniques; D002250:Carbon Radioisotopes; D003609:Dactinomycin / Q000096:biosynthesis*; D014644:Genetic Variation; D007532:Isoleucine / Q000378:metabolism; D010084:Oxidation-Reduction; D011523:Protoplasts / Q000502:physiology*; D013302:Streptomyces / Q000378:metabolism*
D002250:Carbon Radioisotopes; D007532:Isoleucine; D003609:Dactinomycin
Vosstanovlenie biosinteza aktinomitsina pri regeneratsii protoplastov neaktivnogo varianta produtsenta.