Voltage-activated and stretch-activated Ba2+ conducting channels in an osteoblast-like cell line (UMR 106).
Calcium entry may mediate osteoblast activation by calciotropic hormones or changes in electrical fields or mechanical stress in bone. Using cell-attached patch clamping techniques, we have seen two Ba2+ conducting channels in UMR-106 osteoblast-like cells: (i) a voltage-dependent, dihydropyridine-sensitive channel resembling L-type Ca2+ channels in other cells, and (ii) a stretch-activated non-selective channel resembling those involved in mechanoreception and triggering of volume regulation in other cells.
Duncan
R
R
Department of Internal Medicine (Jewish Hospital), St. Louis, MO 63110.
Misler
S
S
D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / Q000494:pharmacology; D000818:Animals; D001464:Barium / Q000378:metabolism*; D004553:Electric Conductivity; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects; D010006:Osteoblasts / Q000502:physiology*; D012516:Osteosarcoma; D011188:Potassium / Q000378:metabolism; D051381:Rats; D012964:Sodium / Q000378:metabolism; D014407:Tumor Cells, Cultured
D007473:Ion Channels; D001464:Barium; D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; D012964:Sodium; D011188:Potassium