RNA-binding properties of influenza A virus matrix protein M1.
The matrix protein (M1) of influenza A virus, which has a critical role in viral assembly and can inhibit the viral transcriptase complex, has the ability to bind RNA. The RNA-binding property of M1 is specific for single-stranded RNA, like that of influenza nucleoprotein (NP) and shows similar sensitivity to pH and to salt concentration. M1:RNA complexes are stable, once formed, to competition from excess single-stranded RNA. The possible location of the RNA-binding regions in the M1 protein is discussed.
Wakefield
L
L
Sir William Dunn School of Pathology, University of Oxford, UK.
Brownlee
G G
GG
D000818:Animals; D001345:Autoradiography; D001483:Base Sequence; D001667:Binding, Competitive; D015152:Blotting, Northern; D015153:Blotting, Western; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D009980:Influenza A virus / Q000378:metabolism*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009994:Osmolar Concentration; D010957:Plasmids; D012313:RNA / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic; D014763:Viral Matrix Proteins / Q000302:isolation & purification / Q000378:metabolism*
C031540:M-protein, influenza virus; C488936:M1 protein, Influenza A virus; D014763:Viral Matrix Proteins; D012313:RNA