Antigenic determinant of trichosanthin peptide fragments.
Purified plant protein, Trichosanthin (TCS), was partially digested with chymotrypsin to produce peptide fragments. Two large fragments, I and II, have been isolated and identified as C-terminal peptides located at sequence positions between 79-234 and 107-234. Their molecular weights determined by electrophoretic mobility on SDS gel were 16,000 and 14,000 daltons. Studies using fluorescence quenching measurements by titrating anti-TCS Fab with TCS and fragment I and II showed that four epitopes were present in intact TCS and one each epitope in fragment I and II. One of the epitopes is therefore located between sequence positions 107 and 234.
Ke
Y P
YP
Shanghai Institute of Cell Biology, China.
Wang
Y H
YH
Yao
Z
Z
Wang
K M
KM
D000019:Abortifacient Agents / Q000276:immunology*; D000020:Abortifacient Agents, Nonsteroidal / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D000939:Epitopes / Q000032:analysis*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology*; D010940:Plant Proteins / Q000276:immunology*; D011817:Rabbits; D015978:Trichosanthin
D000019:Abortifacient Agents; D000020:Abortifacient Agents, Nonsteroidal; D000939:Epitopes; D010446:Peptide Fragments; D010940:Plant Proteins; D015978:Trichosanthin