Cloning and nucleotide sequence analysis of complementary deoxyribonucleic acid for bovine preproinsulin.
A cDNA library was prepared from poly(A+) RNA isolated from fetal bovine pancreas. Bacterial colonies were screened for sequences homologous to a rat preproinsulin I cDNA probe. Ten positive clones were selected at random and further studied. Northern blot analyses revealed that seven of these clones hybridized to a single RNA species, of approximately 400 nucleotides. Sequence analysis of one of these clones (pbI2885) revealed the entire structural region of bovine preproinsulin mRNA including a 72 nucleotide region encoding a signal peptide enriched in hydrophobic residues. The overall nucleotide homology between bovine and human preproinsulin mRNA was 76% for the preregion, 89% for the A chain, 83% for the B chain, and 68% for the C peptide (including a 15 nucleotide deletion).
D'Agostino
J
J
Department of Obstetrics and Gynecology, Baylor College of Medicine, Houston, Texas 77030.
Younes
M A
MA
White
J W
JW
Besch
P K
PK
Field
J B
JB
Frazier
M L
ML
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D005333:Fetus; D007328:Insulin; D007515:Islets of Langerhans / Q000378:metabolism; D008969:Molecular Sequence Data; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D011384:Proinsulin / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger
D007328:Insulin; D011498:Protein Precursors; D012333:RNA, Messenger; D011061:Poly A; C013737:preproinsulin; D012313:RNA; D004247:DNA; D011384:Proinsulin