An abundant testis RNA species shows sequence similarity to Y chromosomal and other genomic sites in Drosophila hydei.
A cDNA clone bank was constructed from testis poly(A)+ RNA of Drosophila hydei and screened for clones which hybridize to Y chromosomal DNA sequences. The insert of clone cDhT14 hybridizes to a family of repeated DNA sequences with members distributed within the Y chromosome and elsewhere in the genome. This type of sequence has earlier been described as the Y-associated class of DNA. Southern blot analysis of DNA from different wild-type strains of D. hydei suggests that members of the T14 family of repeated DNA sequences are parts of a family of transposable elements. The genomic localization of the T14 family of repeated DNA sequences was revealed by in situ hybridization to metaphase and polytene chromosomes, and to transcripts of Y chromosomal lampbrush loops. Approximately 10-15 members (20%-30%) of the T14 sequence family reside in 8.3 kb PstI restriction fragments. A genomic clone of one of these DNA fragments, DhT14-8.3, hybridizes to transcripts on the Y chromosomal lampbrush loop "cones", and in conventional in situ hybridization experiments to region 12D/13A of the X chromosome and to region 112 of chromosome 5. The cDNA clone cDhT14 represents a part of an abundant testis RNA species of 5.0 kb. This RNA is also present in ovaries and in 0-3 h, 3-6 h and 6-12 h embryos, but less abundantly than in testes. Both the Y chromosomal site of the 8.3 kb PstI fragments and sites elsewhere in the genome are actively transcribed. At least one of the latter genomic sites is transcribed into the 5.0 kb RNA species.(ABSTRACT TRUNCATED AT 250 WORDS)
Brand
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RC
Department of Molecular and Developmental Genetics, Faculty of Sciences, University of Nijmegen, The Netherlands.
Hennig
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D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004251:DNA Transposable Elements; D004330:Drosophila / Q000235:genetics*; D005260:Female; D008297:Male; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D013737:Testis / Q000378:metabolism*; D014158:Transcription, Genetic; D014998:Y Chromosome / Q000648:ultrastructure
D004251:DNA Transposable Elements; D012313:RNA; D004247:DNA