title: Spontaneous in vitro neoplastic evolution of cultured Chinese hamster cells. Nucleolus organizing region activity.
abstract: Silver staining to demonstrate active nucleolus organizing regions (NORs) was performed at four different stages of the spontaneous tumorigenic progression in vitro of Chinese hamster WCHE/5 cells. The number of active NORs increased for fully transformed, highly tumorigenic, late passage cells. The increase of NOR material was due to additional NOR-bearing chromosomes or chromosome arms, i.e., trisomy 5, trisomy 8, and the marker chromosome i(3q). Intermediate stages of the neoplastic evolution showed changing patterns of NOR activity, but not an overall increase. We postulate that the increase of active rDNA enhances cell growth and provides undefined selective advantage, and that this supports our previous conclusion that selectable karyotype changes provide competitive advantages rather than being essential for neoplastic evolution in vitro.
authors: Los Alamos National Laboratory, Cell Biology Group, NM 87545.
authors: T
authors: T
authors: Schwarzacher-Robinson
authors: P M
authors: PM
authors: Kraemer
authors: L S
authors: LS
authors: Cram
mesh_terms: D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000473:pathology / Q000648:ultrastructure*; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004171:Diploidy; D007621:Karyotyping; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D012835:Silver Nitrate; D013194:Staining and Labeling; D013997:Time Factors
chemical_list: D012835:Silver Nitrate