[Monoclonal antibodies against the beta subunit of human chorionic gonadotropin].
The aim of investigation was creation of hybridomas, which produce monoclonal antibodies to the beta-subunit of human chorionic gonadotropin (beta-hCG), characterization of monoclonal antibodies, which necessary for hCG immunoassay in biological fluids, as an immunological methods of detection of early stage of pregnancy and choriocarcinoma. 4 hybridomas, producing monoclonal antibodies to-hCG of IgG1 isotype, were created. On the base of monoclonal antibodies, which produced by D2 hybridoma cell line, test-systems for RIA of hCG in blood serum and urine were elaborated. These test-systems can be used in medical practice for diagnosis of early stages of pregnancy and choriocarcinoma.
Fidler
R
R
Sokolov
A V
AV
Verbitskiĭ
M Sh
MSh
Papazov
I P
IP
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification; D000918:Antibody Specificity; D006063:Chorionic Gonadotropin / Q000032:analysis / Q000276:immunology*; D018997:Chorionic Gonadotropin, beta Subunit, Human; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D007132:Immunoglobulin Isotypes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000276:immunology*; D011247:Pregnancy; D011259:Pregnancy Tests, Immunologic; D014328:Trophoblastic Neoplasms / Q000175:diagnosis; D014594:Uterine Neoplasms / Q000175:diagnosis
D000911:Antibodies, Monoclonal; D006063:Chorionic Gonadotropin; D018997:Chorionic Gonadotropin, beta Subunit, Human; D007132:Immunoglobulin Isotypes; D010446:Peptide Fragments
Monoklonal'nye antitela protiv beta-sub''edinitsy khorionicheskogo gonadotropina cheloveka.