Molecular cloning of mouse protein kinase C (PKC) cDNA from Swiss 3T3 fibroblasts.
A set of complementary DNAs (cDNAs) that encode the complete 672-amino acid sequence for mouse protein kinase C (PKC) type alpha have been isolated from a mouse Swiss 3T3 cDNA library. Extensive rescreening of this cDNA library only resulted in the isolation of clones of the same PKC species, indicating that Swiss 3T3 fibroblasts express exclusively PKC-alpha. This enzyme is encoded by two different mRNAs that exhibit a substantial heterogeneity in their 3'-noncoding regions. This heterogeneity could have been derived from alternative splicing of the pre-mRNAs or be due to differential usage of the polyadenylation motif. The expression of PKC mRNA in fibroblasts is very low and it is not influenced by treatment with the tumor promoter 12-O-tetradecanoyl-13-phorbol-acetate.
Rose-John
S
S
Department of Biochemistry, German Cancer Research Center, Heidelberg.
Dietrich
A
A
Marks
F
F
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D005347:Fibroblasts / Q000201:enzymology; D005786:Gene Expression Regulation; D051379:Mice; D008969:Molecular Sequence Data; D011061:Poly A; D014176:Protein Biosynthesis; D011493:Protein Kinase C / Q000235:genetics*; D012313:RNA / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA; D011493:Protein Kinase C