Rapid densitometric determination of cell migration and cell adhesion in a microchemotaxis chamber.
A new rapid staining and measuring method has been developed for the quantification of migrated cells in a microchemotaxis chamber. The migrated cells were, after staining, evaluated by a transmission densitometer. The method introduced here is more accurate and faster than those described previously. In addition the technique can be used to determine the adherent capacity of cells.
Klomp
J P
JP
Division of Immunology, The Netherlands Cancer Institute, Amsterdam.
Te Velde
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D002448:Cell Adhesion; D002465:Cell Movement; D002634:Chemotaxis, Leukocyte; D003720:Densitometry; D006098:Granulocytes / Q000166:cytology; D006801:Humans; D066298:In Vitro Techniques; D008545:Melanoma / Q000473:pathology; D009000:Monocytes / Q000166:cytology; D013194:Staining and Labeling; D014407:Tumor Cells, Cultured