title: Disinfection of Goldmann tonometers against human immunodeficiency virus type 1.
abstract: Goldmann tonometer tips were inoculated with 5 X 10(5) IU of cell-free or cell-associated human immunodeficiency virus type 1 (lymphadenopathy virus type 1 isolate) or 10(4) plaque-forming units of herpes simplex virus type 1 (McKrae strain) or type 2 (Hicks strain). In an effort to mimic a "worst case" clinical scenario, each respective virus was allowed to air dry on the tonometer tip for 10 minutes. Inoculated tonometers were then (1) not treated, (2) wiped with a disposable (Kim-wipe) tissue or sterile gauze; (3) wiped with sterile gauze soaked with 3% hydrogen peroxide; or (4) wiped with a 70% isopropyl alcohol swab. The hydrogen peroxide treatment and the alcohol wipes both completely disinfected the tonometer tips for human immunodeficiency virus type 1 and herpes simplex virus types 1 and 2, whereas wiping with a sterile gauze or tissue was not effective. Wiping the Goldmann tonometer tip with an isopropyl alcohol swab and then allowing the alcohol to evaporate provides a ready and efficient means of inactivating these three enveloped viruses.
authors: Department of Ophthalmology, Washington University School of Medicine, St. Louis, Mo 63110.
authors: J S
authors: JS
authors: Pepose
authors: G
authors: G
authors: Linette
authors: S F
authors: SF
authors: Lee
authors: S
authors: S
authors: MacRae
mesh_terms: D000433:1-Propanol / Q000008:administration & dosage / Q000627:therapeutic use*; D004203:Disinfection / Q000379:methods*; D015488:HIV Antigens / Q000032:analysis; D015497:HIV-1 / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification; D006861:Hydrogen Peroxide / Q000008:administration & dosage / Q000627:therapeutic use*; D008213:Lymphocyte Activation; D008214:Lymphocytes; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D018139:Simplexvirus / Q000187:drug effects / Q000302:isolation & purification; D013242:Sterilization / Q000379:methods*; D014065:Tonometry, Ocular / Q000295:instrumentation*
chemical_list: D015488:HIV Antigens; D000433:1-Propanol; D006861:Hydrogen Peroxide; D012194:RNA-Directed DNA Polymerase