The neutrophil-activating peptide NAF/NAP-1 induces histamine and leukotriene release by interleukin 3-primed basophils.
IgE-independent mediator release from basophils is considered an important event in inflammation, particularly in nonallergic immediate hypersensitivity and in allergic late-phase reactions. This study demonstrates that after exposure to IL-3, basophils release histamine and leukotrienes in response to the neutrophil-activating peptide NAF/NAP-1. Thus, the sequential action of two pure cytokines can promote basophils mediator release. In the presence of IL-3, NAF/NAP-1 functions like a "histamine-releasing factor" and may therefore not only induce cellular infiltration but also provoke symptoms of hypersensitivity reactions.
Dahinden
C A
CA
Institute of Clinical Immunology, University of Bern, Switzerland.
Kurimoto
Y
Y
De Weck
A L
AL
Lindley
I
I
Dewald
B
B
Baggiolini
M
M
D001491:Basophils / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D005966:Glucuronidase / Q000378:metabolism; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D007073:Immunoglobulin E / Q000502:physiology; D066298:In Vitro Techniques; D007377:Interleukin-3 / Q000494:pharmacology*; D016209:Interleukin-8; D009504:Neutrophils / Q000502:physiology; D010455:Peptides / Q000494:pharmacology*; D013189:SRS-A / Q000378:metabolism*
D007377:Interleukin-3; D016209:Interleukin-8; D010455:Peptides; D013189:SRS-A; D007073:Immunoglobulin E; D005966:Glucuronidase