[Antigenic activity of porcine muscle lactate dehydrogenase fragment 180-214 containing the histidine residue of the isoenzyme active center].
Solid phase immunoenzymatic analysis was used to study the antigenic activity of proteolytic degradation products of the porcine muscle lactate dehydrogenase isoform M4. The presence in the enzyme structure of topographic (linear) antigenic determinants was demonstrated. Peptide 180-214 containing histidine-195 in the active center of lactate dehydrogenase was isolated from the tryptic hydrolysate of the carboxymethylated enzyme. This peptide interacts with antibodies against the native enzyme, i.e., antibodies bound to the immunoadsorbent, and causes a 20-25% inhibition of the antigen-antibody complex formation. Protein modification by fluorescein mercuriacetate at Cys-165 essential for the enzyme activity does not result in the synthesis of antibodies that would stimulate the inhibition of the lactate dehydrogenase catalytic activity as compared to antibodies to the native isoenzyme. The putative role of some amino acid residues in the structure of antigenic determinants of porcine muscle lactate dehydrogenase is discussed.
Grebenshchikova
O G
OG
Andrianova
L E
LE
Prozorovskiĭ
V N
VN
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000941:Antigens / Q000276:immunology*; D001665:Binding Sites; D000939:Epitopes / Q000032:analysis; D006639:Histidine / Q000032:analysis*; D006868:Hydrolysis; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000276:immunology*; D009132:Muscles / Q000201:enzymology*; D010446:Peptide Fragments / Q000276:immunology*; D013552:Swine; D014357:Trypsin / Q000378:metabolism
D000596:Amino Acids; D000941:Antigens; D000939:Epitopes; D007527:Isoenzymes; D010446:Peptide Fragments; D006639:Histidine; D007770:L-Lactate Dehydrogenase; D014357:Trypsin
Antigennaia aktivnost' fragmenta 180-214 laktatdegidrogenazy iz myshts svin'i, soderzhashchego gistidin aktivnogo tsentra izofermenta.