Serotypic analysis of VP3 and VP7 neutralization escape mutants of rhesus rotavirus.
Neutralization escape mutants of simian rotaviruses (rhesus rotavirus and SA11) were tested in hemagglutination inhibition and neutralization assays against hyperimmune and infection sera to determine if mutation in an immunodominant epitope could enable neutralization escape. An SA11 mutant with a new glycosylation site at amino acid 211 of VP7 was shown to escape neutralization by hyperimmune but not infection sera.
Shaw
R D
RD
Department of Medicine, Stanford University School of Medicine, California.
Mackow
E R
ER
Dyall-Smith
M L
ML
Lazdins
I
I
Holmes
I H
IH
Greenberg
H B
HB
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000096:biosynthesis*; D002213:Capsid / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D006168:Guinea Pigs; D006385:Hemagglutination Inhibition Tests; D008253:Macaca mulatta; D051379:Mice; D009154:Mutation; D009500:Neutralization Tests; D012401:Rotavirus / Q000235:genetics / Q000276:immunology*; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000939:Epitopes