Human monoclonal IgG1 insulin autoantibody from insulin autoimmune syndrome directed at determinant at asparagine site on insulin B-chain.
Purified human insulin autoantibody (IAA) collected from the serum of a man (T.H.) with insulin autoimmune syndrome was characterized. The TH-IAA was found to be of IgG1 (lambda-light-chain) subclass. In addition to the single-binding affinity of TH-IAA to human insulin that we have shown in previous studies, the TH-IAA binding to human insulin was completely inhibited by a mouse monoclonal anti-idiotypic antibody against TH-IAA. A competitive inhibition study with various insulins revealed an epitope of human insulin against TH-IAA. These findings suggest that TH-IAA is monoclonal and is directed at a determinant at B-3 (asparagine) on the human insulin B-chain.
Uchigata
Y
Y
Diabetes Center, Tokyo Women's Medical College, Japan.
Yao
K
K
Takayama-Hasumi
S
S
Hirata
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001216:Asparagine; D001323:Autoantibodies / Q000276:immunology; D001327:Autoimmune Diseases / Q000276:immunology*; D002417:Cattle; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007163:Immunosorbent Techniques; D007328:Insulin / Q000276:immunology*; D007330:Insulin Antibodies / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D051381:Rats; D013045:Species Specificity; D013552:Swine
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D000939:Epitopes; D007328:Insulin; D007330:Insulin Antibodies; D001216:Asparagine