A double staining method for the detection of a macrophage surface marker and nonspecific esterase activity.
A double staining method for the detection on macrophages of a surface marker (Ia molecules) by immunofluorescence and of non-specific esterase activity is described. This method reveals the co-distribution of both markers in macrophages either fixed in suspension or on coverslips with 1% paraformaldehyde. Good resolution of both markers is obtained and the method should be useful for characterizing mononuclear phagocytic cells in heterogeneous cell preparations.
Leyva-Cobián
F
F
Servicio de Immunología, Centro Ramón y Cajal, Madrid, Spain.
Flores
R
R
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D008264:Macrophages / Q000032:analysis* / Q000145:classification / Q000201:enzymology; D051379:Mice; D008808:Mice, Inbred CBA; D010529:Peritoneal Cavity / Q000166:cytology; D010641:Phenotype; D013194:Staining and Labeling / Q000379:methods*
D000954:Antigens, Surface; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase