title: Enhancement of the histamine releasing activity of mouse monoclonal anti-human IgE antibody xb6-16 when present in aggregated or complexed forms.
abstract: FPLC purification of mouse monoclonal anti-human IgE antibody xb6-16 showed 2 major peaks of different molecular weight, peak 1 (greater than 10(6) d) and peak 3 (1.6 x 10(5) d). Peak 1 consisted of IgG1 and IgM, peak 3 of IgG1 only. On a protein weight basis, peak 1 was 100 times more potent than peak 3 in inducing histamine release from human basophils. Preincubation of peak 3 with anti-IgG1 enhanced the mediator release triggered by this fraction. On this basis, the potentiating effect of aggregated IgG1 or IgG1-IgM complexes on mediator release from basophils is discussed.
authors: Unité d'Immuno-Allergie, Institut Pasteur, Paris, France.
authors: A
authors: A
authors: Weyer
authors: J P
authors: JP
authors: Dandeu
authors: M
authors: M
authors: Etiévant
authors: J P
authors: JP
authors: Bourgeois
authors: F
authors: F
authors: Marchand
authors: A
authors: A
authors: Prouvost-Danon
authors: B
authors: B
authors: David
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D001491:Basophils / Q000276:immunology / Q000378:metabolism; D006636:Histamine Release; D006801:Humans; D007073:Immunoglobulin E; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D066298:In Vitro Techniques; D051379:Mice; D008970:Molecular Weight
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007073:Immunoglobulin E