[Cultivation of cells producing the human immunodeficiency virus].
The possibility of optimizing the conditions for the cultivation of cells producing human immunodeficiency virus (HIV) was explored. The stimulating effect on the cell cultures of interleukin-2 and specific anti-interferon antibodies was examined. The individual use of interleukin-2 or anti-interferon antibody preparations did not result in any marked enhancement of HIV virus reproduction in the cells, whereas combining of interleukin-2 which stimulated proliferation of T-lymphocytes with poly- and monoclonal anti-interferon antibodies proved to be effective increasing the expression of virus-specific antigens in the cells 1.5-fold. It seems expedient to carry out further screening of different reagents and combinations thereof capable of significantly increasing HIV virus reproduction in cell cultures which would serve as the antigen for diagnostic systems.
Zhdanov
V M
VM
Gromyko
A I
AI
Ershov
F I
FI
Nosik
D N
DN
Korneeva
M N
MN
D000906:Antibodies / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D002460:Cell Line; D005347:Fibroblasts / Q000187:drug effects / Q000276:immunology / Q000382:microbiology*; D006678:HIV / Q000254:growth & development* / Q000276:immunology; D006801:Humans; D007372:Interferons / Q000276:immunology; D007376:Interleukin-2 / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000382:microbiology*; D013268:Stimulation, Chemical; D014776:Virus Cultivation / Q000379:methods; D014779:Virus Replication / Q000187:drug effects
D000906:Antibodies; D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D007372:Interferons
Kul'tivirovanie kletok-produtsentov virusa immunodefitsita cheloveka.