title: Functional characterization of a voltage-gated anion channel from teleost fish intestinal epithelium.
abstract: An anion channel was isolated, using patch-clamp technique, from the basolateral membrane of goby intestinal epithelial cells. Single-channel conductance varied over a range from 20 to 90 pS. The channel was voltage-gated over the physiological range of cell membrane potential with depolarization increasing the proportion of time in the open state. There was no Ca2+ sensitivity. The selectivity sequence was SO4(2-) greater than Cl- greater than Mes-. The channel may function in vivo as one of several avenues of basolateral membrane Cl- exit with the voltage-gating property serving to match basolateral Cl- exit to apical entry.
authors: Department of Biological Sciences, State University of New York, Buffalo 14260.
authors: C A
authors: CA
authors: Loretz
authors: C R
authors: CR
authors: Fourtner
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002712:Chlorides / Q000378:metabolism*; D004594:Electrophysiology; D004848:Epithelium / Q000378:metabolism / Q000648:ultrastructure; D005399:Fishes / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000648:ultrastructure; D007473:Ion Channels / Q000502:physiology*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning
chemical_list: D002712:Chlorides; D007473:Ion Channels