A patch-clamp study of ion channels of inner and outer membranes and of contact zones of E. coli, fused into giant liposomes. Pressure-activated channels are localized in the inner membrane.
Inner and outer membranes of Escherichia coli and contact zones were isolated and fused separately with giant liposomes amenable to patch-clamp recording. Different types of large pressure-activated channels were localized in the inner membrane fraction which also contained smaller, pressure-insensitive channels. The outer membrane contained pressure-insensitive channels with large conductances and long opening and closing times which are likely to be porins. Large channels were also observed in contact zones.
Berrier
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C
Laboratoire des Biomembranes, URA CNRS 1116, Orsay, France.
Coulombe
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A
Houssin
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C
Ghazi
A
A
D002458:Cell Fractionation; D002462:Cell Membrane / Q000502:physiology; D004553:Electric Conductivity; D004926:Escherichia coli / Q000502:physiology* / Q000648:ultrastructure; D006874:Hydrostatic Pressure; D007473:Ion Channels / Q000502:physiology*; D008081:Liposomes; D008564:Membrane Potentials
D007473:Ion Channels; D008081:Liposomes