title: Distribution of T cells bearing different forms of the T cell receptor gamma/delta in normal and pathological human tissues.
abstract: Frozen sections from normal and pathologic human tissues were immunostained by the APAAP technique with three mAb directed against different epitopes of the TCR gamma delta; TCR delta 1 which binds to all cells bearing the TCR gamma delta; BB3 and delta TCS1 which, by immunoprecipitation studies, appear to react respectively with the disulfide-linked and nondisulfide-linked form of the TCR gamma delta. In normal thymus, TCR delta 1+ cells accounted for approximately 2% of the CD3+ thymocytes and were about three times more numerous in the medulla than in the cortex. TCR delta 1+ cells were mostly constituted by the delta TCS1 reactive subset (average ratio delta TCS1/BB3: 3.7). In the tonsil, the TCR delta 1+ cells (about 3% of CD3+ elements) were mainly located in the interfollicular area, where they frequently tended to arrange around high endothelium venules. In most samples, TCR delta 1+ cells were distributed beneath to the tonsil epithelium. Unlike thymus, the majority of TCR delta 1+ cells were usually constituted by the BB3-reactive subset (average BB3/delta TCS1 ratio: 2.0). A similar predominance of BB3+ over delta TCS1+ cells was also observed in normal peripheral blood. The spleen was the organ with the highest concentration of TCR delta 1+ cells that, like in the thymus, were mostly represented by delta TCS1+ elements. Noteworthy, the TCR delta 1+ cells were preferentially located in the splenic sinusoids while TCR alpha beta-bearing lymphocytes mostly occupied the periarteriolar sheaths of penicilliary arteries. The majority of neoplastic T cell proliferations studied lacked to express the TCR gamma delta. Two cases of beta F1-(TCR alpha beta-) T lymphoblastic lymphoma, however, were TCR gamma delta+ (delta TCS1+/BB3-). Both of them showed a stage II cortical phenotype, e.g., CD1+/CD3+/CD4+/CD8+/TCR delta 1+. Among inflammatory conditions, an increase of BB3+ cells was observed in close association with necrotic areas in cases of Kikuchi's and tuberculous lymphadenitis. The significance of this finding is under study.
authors: Institute of Internal Medicine, University of Perugia, Italy.
authors: B
authors: B
authors: Falini
authors: L
authors: L
authors: Flenghi
authors: S
authors: S
authors: Pileri
authors: P
authors: P
authors: Pelicci
authors: M
authors: M
authors: Fagioli
authors: M F
authors: MF
authors: Martelli
authors: L
authors: L
authors: Moretta
authors: E
authors: E
authors: Ciccone
mesh_terms: D000911:Antibodies, Monoclonal; D002648:Child; D006801:Humans; D007150:Immunohistochemistry; D008223:Lymphoma / Q000032:analysis / Q000473:pathology; D014066:Palatine Tonsil / Q000378:metabolism / Q000473:pathology; D011948:Receptors, Antigen, T-Cell / Q000032:analysis*; D013154:Spleen / Q000378:metabolism / Q000473:pathology; D013194:Staining and Labeling; D013601:T-Lymphocytes / Q000378:metabolism* / Q000473:pathology; D013950:Thymus Gland / Q000378:metabolism / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D011948:Receptors, Antigen, T-Cell