title: Detection of proteinase inhibitors in fractions after liquid chromatography.
abstract: A technique for rapid detection of proteinase inhibitors in fractions after liquid chromatography is described. Aliquots of the tested solutions are placed onto a thin layer of gelatin, crosslinked with glutardialdehyde in the presence of water soluble nigrosin, to form small drops. A proteinase solution is added to the above-mentioned drops and the plate is incubated at ambient temperature for approximately 15-25 min. After the gelatin layer is washed with water the inhibitor negative fractions are visualized as colorless zones on a blue background. With inhibitor positive fractions no change of gelatin layer occurs.
authors: Laboratory of Analytical Chemistry, South Bohemian Biological Centre, Ceské Budĕjovice, Czechoslovakia.
authors: I
authors: I
authors: Safarík
mesh_terms: D007611:Aprotinin / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D005782:Gels; D011480:Protease Inhibitors / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005782:Gels; D011480:Protease Inhibitors; D007611:Aprotinin