Cercopithecus aethiops monkey as a reliable model for in vitro study of T cell depletion of bone marrow with Campath-1 plus complement.
T-lymphocyte markers of peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs) of C. aethiops monkeys were studied by using anti-human monoclonal antibodies. The results show that C. aethiops T lymphocytes express surface markers which react specifically with anti-human MoAbs including CD3, CD4, CD8, CD2. However, very few CD3-positive cells were found, in contrast to the abundance in CD8+ cells. There is a high conservation of receptors forming E rosettes with AET-treated SRBCs, and antigens reacting with the anti-human T and B cell monoclonal antibody (Campath-1). The present findings indicate that C. aethiops can be used as a new experimental model for studies on T-cell depletion from bone marrow with Campath-1 MoAb + rabbit C.
Phan
D T
DT
National Institute of Haematology and Blood Transfusion, Budapest, Hungary.
Benczur
M
M
Gidáli
J
J
Fehér
I
I
Harsányi
V
V
Nemes
L
L
Petrányi
G
G
Hollán
S R
SR
D000818:Animals; D000911:Antibodies, Monoclonal; D001772:Blood Cell Count; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003114:Colony-Forming Units Assay; D003165:Complement System Proteins; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008212:Lymphocyte Depletion; D008297:Male; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology
D000911:Antibodies, Monoclonal; D003165:Complement System Proteins