Optimal temperature for synthesis of DNA, RNA, and protein by human testis in vitro.
To determine the optimal temperature for DNA, RNA, and protein syntheses in the human testis, the levels of incorporation of 3H-thymidine, 14C-uridine, and 14C-leucine into testicular tissue were studied at 28 degrees C, 31 degrees C, 34 degrees C, and 37 degrees C in vitro. The results suggest that the DNA synthesis is at a maximum at 31 degrees C and shows delicate temperature sensitivity, whereas the RNA and protein syntheses are at a maximum at 34 degrees C or 37 degrees C and are temperature-dependent. It is concluded that the temperature sensitivity of DNA synthesis may be one of the primary causes of delicate thermal inhibition of human spermatogenesis.
Nakamura
M
M
Department of Urology, School of Medicine, Osaka University, Japan.
Namiki
M
M
Okuyama
A
A
Koh
E
E
Kondoh
N
N
Takeyama
M
M
Fujioka
H
H
Nishimune
Y
Y
Matsumoto
K
K
Matsuda
M
M
D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D006801:Humans; D066298:In Vitro Techniques; D007930:Leucine / Q000378:metabolism; D008297:Male; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*; D013696:Temperature; D013737:Testis / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
D012313:RNA; D004247:DNA; D007930:Leucine; D013936:Thymidine; D014529:Uridine