Establishment of human malignant mesothelioma cell lines.
Seventeen human malignant mesothelioma cell lines were isolated from 61 samples (46 effusions, 9 biopsies and 6 tumors obtained at autopsy) collected from patients with a confirmed malignant mesothelioma. The method used is given in detail. Cytogenetic analysis of growing cultures is the best indicator to determine whether the observed proliferation concerns malignant or normal mesothelial cells. The addition of epidermal growth factor (EGF) and hydrocortisone (HC), or EGF alone, to the culture medium increases the chances of successful isolation of a malignant mesothelioma cell line.
Versnel
M A
MA
Department of Cell Biology, Immunology and Genetics, Erasmus University, Rotterdam, The Netherlands.
Bouts
M J
MJ
Hoogsteden
H C
HC
van der Kwast
T H
TH
Delahaye
M
M
Hagemeijer
A
A
D000818:Animals; D002869:Chromosome Aberrations; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D007633:Keratins / Q000032:analysis; D008654:Mesothelioma / Q000235:genetics / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011519:Proto-Oncogenes; D014407:Tumor Cells, Cultured
D004815:Epidermal Growth Factor; D007633:Keratins; D006854:Hydrocortisone