Quantitation of HIV-1 activity in tissue culture supernatants: effects of culture condition on syncytial assays and virus production.
We have compared the infectivity titres, reverse transcriptase levels and antigen titres in the supernatants from persistently infected cell lines that produce a variety of HIV-1 isolates. We found a poor correlation between the different assays, and a variability in viral activity dependent on culture medium.
Spickett
G
G
Department of Immunological Medicine, Clinical Research Centre, Harrow, Oxford, U.K.
Beattie
R E
RE
Bountiff
L
L
Dalgleish
A G
AG
Webster
A D
AD
D002459:Cell Fusion; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D004795:Enzyme Stability; D015488:HIV Antigens / Q000032:analysis; D015497:HIV-1 / Q000254:growth & development / Q000502:physiology*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013045:Species Specificity; D014779:Virus Replication
D003470:Culture Media; D015488:HIV Antigens; D012194:RNA-Directed DNA Polymerase