Induction by chemical clastogens of aberrations in prematurely condensed interphase chromatin of Chinese hamster ovary cells.
The clastogenic activities of diepoxybutane and bleomycin were comparatively studied on prematurely condensed interphase chromatin and metaphase chromosomes of Chinese hamster ovary cells. The yield of chromosomal aberrations was distinctly higher in G2-premature chromosome condensation as compared to metaphase. Most notably, the clastogenic activity of bleomycin was visible in premature chromosome condensation after application of much lower final concentrations than necessary for induction of chromosome aberrations in metaphase. In addition, the different mechanisms of action of both clastogens were reflected by the aberration yield in G1 and G2 immediately after exposure. While bleomycin induced aberrations throughout all stages of interphase, diepoxybutane did not induce aberrations in G1 or G2. Though certainly not a routine system for genotoxicity testing, premature chromosome condensation analyses provide a powerful opportunity to demonstrate relationships between DNA damage and repair, and the production of chromosomal changes at the site of their formation.
Abka'i
F
F
Institute of Human Genetics, University of Erlangen-Nurnberg, West Germany.
Wachter
E
E
Tittelbach
H
H
Gebhart
E
E
D000818:Animals; D001761:Bleomycin / Q000633:toxicity*; D002843:Chromatin / Q000187:drug effects*; D002869:Chromosome Aberrations / Q000187:drug effects*; D006224:Cricetinae; D003412:Cricetulus; D004852:Epoxy Compounds / Q000633:toxicity*; D004988:Ethers, Cyclic / Q000633:toxicity*; D005260:Female; D007399:Interphase; D009153:Mutagens; D010053:Ovary / Q000166:cytology
D002843:Chromatin; D004852:Epoxy Compounds; D004988:Ethers, Cyclic; D009153:Mutagens; D001761:Bleomycin; C007366:diepoxybutane