Association of monoclonal antibody-recognized antigen with steroid receptors in gynecologic tumors.
In an attempt to differentiate between the biochemical characteristics of hormonal-dependent and independent gynecologic tumors, monoclonal antibodies (mABs) MBr1 and MBr3 were used to detect tumor antigens. Steroid receptors and MBr1 and MBr3 determinants were assayed independently in a double-blinded manner. Of the 45 gynecologic tumors analyzed, 16 of 20 (80%) with both progesterone receptors (PR) (greater than 10 fm/mg protein) and estrogen receptors (ER) (greater than 5 fm/mg protein) also contained high levels of MBr1 determinant. However, these receptors were not quantitatively related to MBr1 activity. The 15 of 17 (88%) tumors that contained receptors below the discriminant levels also had low or undetectable levels of MBr1 activity. In tumors where only either PR or ER was present, 2 of 2 PR+/ER- tumors and 2 of 6 PR-/ER+ tumors expressed MBr1 antigen. No relationship of MBr3 antigenic activity to the receptors was established. These results show that MBr1-recognized antigen is expressed largely in steroid receptor-positive tumors and these data suggest that either the antigen itself or its carrier protein may be regulated by estrogen. Like steroid receptors, MBr1 antigens may have important prognostic value in gynecologic tumors.
Wang
K C
KC
Department of Obstetrics and Gynecology, University of Minnesota, Minneapolis 55455.
Leung
B S
BS
Colnaghi
M I
MI
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D005260:Female; D005470:Fluorometry; D005833:Genital Neoplasms, Female / Q000032:analysis*; D006801:Humans; D011960:Receptors, Estrogen / Q000032:analysis*; D011980:Receptors, Progesterone / Q000032:analysis*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000939:Epitopes; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone