Binding and biological actions of prostaglandin E2 and I2 in cells isolated from rabbit gastric mucosa.
1. We have investigated the binding of the tritiated forms of prostaglandin E2 (PGE2) and a stable analogue of prostacyclin (Iloprost) to isolated cells of rabbit oxyntic mucosa. 2. The highest degree of specific [3H]PGE2 binding occurred in a cellular fraction enriched in parietal cells. [3H]Iloprost binding occurred predominantly in cells identified as mucous cells. 3. PGE2 binding to the parietal cell fraction was associated with its ability to inhibit histamine-stimulated aminopyrine accumulation by these cells. Iloprost binding did not correlate with a biological action on the parietal cells. 4. PGE2 and Iloprost reduced Trypan Blue staining in cells exposed to 10% (w/v) ethanol. Iloprost (10(-8) to 10(-6) M) reduced Trypan Blue staining in cells identified as mucous and parietal cells. PGE2 (10(-8) M) significantly reduced Trypan Blue staining in parietal cell-enriched fractions. 5. Cyclic AMP stimulation in response to either prostanoid occurred most potently on non-parietal cell fractions. However PGE2 or Iloprost binding affinities did not correlate with cyclic AMP formation. 6. These data provide evidence for true PGE2 receptors on oxyntic mucosal cells. The receptors appear to mediate inhibition of acid secretion. Iloprost binds to sites which might mediate cellular protection.
Barr
D B
DB
Department of Physiology, Faculty of Medicine, University of Western Ontario, London, Canada.
Duncan
J A
JA
Kiernan
J A
JA
Soper
B D
BD
Tepperman
B L
BL
D000632:Aminopyrine / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000096:biosynthesis; D015232:Dinoprostone / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D011464:Epoprostenol / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism*; D006633:Histamine Antagonists / Q000378:metabolism; D016285:Iloprost; D011817:Rabbits
D006633:Histamine Antagonists; D000632:Aminopyrine; D011464:Epoprostenol; D000242:Cyclic AMP; D016285:Iloprost; D015232:Dinoprostone