Immunostaining of tissue sections previously stained with hematoxylin and eosin.
S-100 protein was demonstrated in 28/31 melanoma tissue sections that had been previously stained by hematoxylin and eosin, including 17 from 19 primary melanomas and 11 from 12 metastases. Additional H & E stained sections from 11 metastatic and 13 primary melanomas were reacted with the monoclonal antibody NKI/C-3 and all stained positively. This approach is of value when additional sections are not available or when deeper cuts through a block do not contain tumor. The superimposition of the immunostaining does not reduce the record and diagnostic value of the double-stained sections.
Paul
E
E
Centre for Dermatology and Andrology, Hautklinik, University of Giessen, Federal Republic of Germany.
Cochran
A J
AJ
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000276:immunology*; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D008545:Melanoma / Q000175:diagnosis* / Q000276:immunology / Q000378:metabolism; D009418:S100 Proteins / Q000276:immunology / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D009418:S100 Proteins; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin