title: Fluid flow stimulates tissue plasminogen activator secretion by cultured human endothelial cells.
abstract: Wall shear stress generated by blood flow may regulate the expression of fibrinolytic proteins by endothelial cells. Tissue plasminogen activator (tPA) and plasminogen activator inhibitor, type 1 (PAI-1) secretion by cultured human endothelial cells were not affected by exposure to venous shear stress (4 dynes/cm2). However, at arterial shear stresses of 15 and 25 dynes/cm2, the tPA secretion rate was 2.1 and 3.0 times greater, respectively, than the basal tPA secretion rate. PAI-1 secretion was unaffected by shear stress over the entire physiological range.
authors: Rice University, Biomedical Engineering Laboratory, Houston, TX 77251.
authors: S L
authors: SL
authors: Diamond
authors: S G
authors: SG
authors: Eskin
authors: L V
authors: LV
authors: McIntire
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000378:metabolism*; D011464:Epoprostenol / Q000494:pharmacology; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D016285:Iloprost; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D015849:Plasminogen Inactivators; D012212:Rheology; D012636:Secretory Rate / Q000187:drug effects; D013314:Stress, Mechanical; D013997:Time Factors; D010959:Tissue Plasminogen Activator / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D015849:Plasminogen Inactivators; D011464:Epoprostenol; D010959:Tissue Plasminogen Activator; D016285:Iloprost; D015056:1-Methyl-3-isobutylxanthine; D007213:Indomethacin