Method for epitope selection of antisera for immunohistology.
Polyclonal anti-laminin serum was affinity-purified on paraformaldehyde-fixed laminin on a nitrocellulose filter. The purified antibodies were tested for their specificity in immunohistological stainings on frozen sections of paraformaldehyde-fixed tissue. As compared to the initial polyclonal serum, the purified antibodies increased the specificity of antigen detection, since all background caused by nonspecific reactions was eliminated. This technique promises to be very useful for immunohistological analysis using light and electron microscopy.
Wedlich
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Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, FRG.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000918:Antibody Specificity; D003101:Collodion; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry / Q000379:methods*; D007797:Laminin / Q000276:immunology* / Q000378:metabolism; D007814:Larva; D011487:Protein Conformation; D014982:Xenopus laevis
D000906:Antibodies; D000939:Epitopes; D007797:Laminin; D003101:Collodion