Effects of calcium channel blockade in canine saphenous veins after storage at -190 degrees C.
1. Canine saphenous veins were investigated in vitro either within 24 h after removal or after storage at -190 degrees C for 4-5 weeks of foetal calf serum containing 1.8 M dimethyl sulphoxide. 2. Contractions and 45Ca2+ uptake in response to both depolarization and guanfacine were studied in the absence and presence of the calcium channel antagonists diltiazem, verapamil, nifedipine and the two stereoisomers of a 1,4-dihydropyridine derivative, namely the (+)-(S) enantiomer and the (-)-(R) enantiomer of 202-791 (isopropyl 4-(2,1,3-benzoxadiazol-4-yl)-1,4-dihydro-2,6-dimethyl-5-nitro-3- pyridinecarboxylate). 3. Comparison of the data obtained on unfrozen and frozen/thawed veins revealed a good preservation of both contractile responsiveness and 45Ca2+ uptake mechanisms after storage at -190 degrees C. 4. It is suggested that cryopreservation is a useful technique for storing venous smooth muscle for pharmacological studies.
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Preclinical Research, Sandoz Ltd., Basel, Switzerland.
Müller-Schweinitzer
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Vogel
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D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004285:Dogs; D005260:Female; D005615:Freezing; D016316:Guanfacine; D006146:Guanidines / Q000494:pharmacology; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects*; D008297:Male; D010648:Phenylacetates / Q000494:pharmacology; D012501:Saphenous Vein / Q000502:physiology*; D014021:Tissue Preservation; D014661:Vasoconstriction / Q000187:drug effects
D000316:Adrenergic alpha-Agonists; D006146:Guanidines; D007473:Ion Channels; D010648:Phenylacetates; D016316:Guanfacine; D002118:Calcium