Application of urinary indicator proteins in the non-invasive assessment of glomerulo-tubular lesions in patients with chronic glomerulonephritis and rheumatoid arthritis.
As the semiautomated electrophoretic analysis of proteinuria still needs technical experience, interest was focused on easy-to-perform methods of urinary protein measurement. SRID-tests for albumin, transferrin, IgG, alpha-1-microglobulin and a spectrophotometrical test for beta-NAG were carried out in 50 normal controls and compared to PCI/ECI-values of patients suffering from rheumatoid arthritis (n = 52) and various types of chronic glomerulonephritis (n = 41). Elevated levels of alpha-1-M and beta-NAG in chronic glomerulonephritis were interpreted as indicative for tubulointerstitial involvement in the chronic inflammatory process. PCI/ECI elevation in individual RA-samples may be caused by functional impairment of tubular protein handling due to chronic ingestion of non-steroid analgesics. The serum assays for transferrin (TF) and IgG based on SRID technique turned out to be too insensitive for the application on unconcentrated urine of normal control persons. In renal patients, however, TF-PCI values above 30 mg/g crea and IgG-PCI values above 50 mg/g crea have to be interpreted as pathologic indicating damage of the glomerular basement membrane. To elucidate TF- and IgG-values in urines with low protein content, highly sensitive nephelometric methods should be used. Concentration of urinary proteins using membrane filters may lead to protein losses, resulting in miscalculation of PC-indices.
Weber
M H
MH
Zentrum Innere Medizin der Universität Göttingen.
Verwiebe
R
R
Janning
G
G
Scheler
F
F
D000118:Acetylglucosaminidase / Q000652:urine; D058186:Acute Kidney Injury / Q000150:complications*; D000328:Adult; D000368:Aged; D000419:Albuminuria; D000510:Alpha-Globulins / Q000652:urine; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000009:adverse effects; D001172:Arthritis, Rheumatoid / Q000150:complications* / Q000188:drug therapy; D001798:Blood Proteins / Q000032:analysis*; D003404:Creatinine / Q000652:urine; D005260:Female; D005921:Glomerulonephritis / Q000139:chemically induced / Q000150:complications*; D006801:Humans; D007074:Immunoglobulin G / Q000652:urine; D007683:Kidney Tubular Necrosis, Acute / Q000139:chemically induced / Q000150:complications*; D008297:Male; D008875:Middle Aged; D011507:Proteinuria / Q000175:diagnosis*; D014168:Transferrin / Q000652:urine
D000510:Alpha-Globulins; D000894:Anti-Inflammatory Agents, Non-Steroidal; D001798:Blood Proteins; D007074:Immunoglobulin G; D014168:Transferrin; C016001:alpha-1-microglobulin; D003404:Creatinine; D000118:Acetylglucosaminidase