[Identification of the neuronal protein with antigenic properties of latrotoxin].
Monospecific antibodies against neurotoxin from the black widow spider venom (alpha-latrotoxin) have been used to identify a neuronal protein (s) with latrotoxin-like epitopes. As determined by ELISA, the protein is localized in cytoplasmic fraction of bovine brain. It was partially purified by anion-exchange chromatography and preparative SDS-electrophoresis. Immunoblotting data indicate that this neuronal acidic protein has molecular mass of ca. 100 kDa.
Tsygankova
O M
OM
Tret'iakov
L A
LA
Grishin
E V
EV
D000818:Animals; D001180:Arthropod Venoms / Q000276:immunology*; D001923:Brain Chemistry; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis*; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000276:immunology*; D013111:Spider Venoms / Q000276:immunology*
D001180:Arthropod Venoms; D000939:Epitopes; D009419:Nerve Tissue Proteins; D013111:Spider Venoms; C020763:alpha-latrotoxin
Identifikatsiia neĭronal'nogo belka s antigennymi svoĭstvami latrotoksina.