Eimeria maxima: isolation of gametocytes and their immunogenicity in mice, rabbits, and chickens.
Eimeria maxima gametocytes were isolated from infected chicken intestinal tissue by treatment with hyaluronidase and subsequent filtration through polymon filters. The isolated gametocytes were analyzed by microscopical and biochemical methods and shown to be highly enriched. The antigenicity of the gametocytes was analyzed in mice, rabbits, and chickens by ELISA and indirect immunofluorescence. Contrary to published results, we have found gametocytes to be highly immunogenic in all animals tested.
Pugatsch
T
T
Kuvin Center for the Study of Infectious and Tropical Diseases, Hebrew University, Hadassah Medical School, Jerusalem, Israel.
Mencher
D
D
Wallach
M
M
D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D000953:Antigens, Protozoan / Q000276:immunology*; D002645:Chickens / Q000469:parasitology*; D003048:Coccidiosis / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D004539:Eimeria / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D007106:Immune Sera / Q000276:immunology; D007114:Immunization; D051379:Mice; D011201:Poultry Diseases / Q000276:immunology / Q000469:parasitology; D012313:RNA / Q000032:analysis; D011817:Rabbits
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007106:Immune Sera; D012313:RNA