Comparison of Papanicolaou's and Wright-Giemsa stains in the examination of body fluids for Hodgkin's disease.
We reviewed 36 body fluid specimens from 18 patients with Hodgkin's disease (HD) to characterize the cytologic features of HD as seen in Wright-Giemsa (WG)-stained cytocentrifuge preparations, and to compare diagnostic agreement between WG- and Papanicolaou-stained samples. Slides were examined independently by two pathologists without knowledge of the original diagnosis, and were classified as either positive, inconclusive, or negative for malignant cells. There was diagnostic agreement between both methods in 35 (97%) of 36 samples. Features in cytocentrifuged WG-stained specimens that were most helpful in recognizing HD included mirror image nuclei in typical Reed-Sternberg cells and an axis of symmetry in polylobate Reed-Sternberg variants, with even distribution of the nuclear material within the cytoplasm.
Wilson
M S
MS
Department of Pathology, Ohio State University, Columbus 43210.
Theil
K S
KS
Goodwin
R A
RA
Brandt
J T
JT
D000328:Adult; D000368:Aged; D001399:Azure Stains; D001826:Body Fluids / Q000166:cytology*; D003937:Diagnosis, Differential; D005069:Evaluation Studies as Topic; D006689:Hodgkin Disease / Q000175:diagnosis / Q000473:pathology*; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000175:diagnosis / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010640:Phenothiazines; D013194:Staining and Labeling / Q000379:methods*
D001399:Azure Stains; D010640:Phenothiazines