Molecular cloning and characterization of alpha-subunit isoforms of the Na,K-ATPase.
The identification of the different alpha-subunit isoforms of the Na,K-ATPase has added an unexpected complexity to the understanding of the function and regulation of this important transport protein. Our results indicate that the alpha, alpha(+) and alpha III isoform mRNAs have distinct, but partially overlapping, distributions in brain and peripheral tissues. Also, characterization of mRNA distribution in fetal tissues suggests that the Na,K-ATPase alpha-subunit isoforms are regulated during development and differentiation. The developmental regulation and strikingly different tissue specificities of the three alpha-subunit isoforms suggests that the different Na,K-ATPase alpha-subunits may be adapted to perform different roles in maintaining sodium and potassium homeostasis.
Mercer
R W
RW
Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO 63110.
Schneider
J W
JW
Benz
E J
EJ
D000818:Animals; D001921:Brain / Q000201:enzymology; D003001:Cloning, Molecular; D005333:Fetus; D005796:Genes; D007527:Isoenzymes / Q000235:genetics*; D007668:Kidney / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D009928:Organ Specificity; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics*; D014158:Transcription, Genetic
D007527:Isoenzymes; D012333:RNA, Messenger; D012313:RNA; D000254:Sodium-Potassium-Exchanging ATPase