title: Inflammatory competence of fetal rat: acute-phase plasma protein response of the fetus treated by turpentine in utero.
abstract: Using crossed immunoelectrophoresis, immunoelectrodiffusion, autoradiography, and equilibrium binding techniques, we demonstrate that the rat fetus, directly challenged in utero at 18 days by a single subcutaneous turpentine injection, presents a complex acute-phase plasma inflammatory response. A number of fetal serum proteins, 48 h after the injection, increase in concentration by factors of about 2-5. These positive acute-phase reactants (APR) are alpha 1-acute-phase globulin (alpha 1-AP), alpha 2-macroglobulin (alpha 2-M), alpha 1-acid glycoprotein (alpha 1-AG), haptoglobin (Hp), and hemopexin (Hpx). A number of proteins decrease, behaving like negative APRs. These are albumin, alpha 1-fetoprotein (AFP), transferrin, GHR-P63, thyroxine-binding prealbumin (TBPA), and transcortin (CBG). The marked fall in concentration of two of the high-affinity hormone-binding proteins of the fetal rat, i.e., the estrophilic AFP and TBPA, induce significant decreases (by 25-40%) of the estrogen- and thyroxine-binding abilities of the fetal serum. While the plasma inflammatory response of the fetus is qualitatively similar to that of the adult, the fetal reactions are, as a rule, quantitatively weaker. The characteristics of the plasma inflammatory response of the fetus are discussed in relation to the highly dynamic state of its development.
authors: U. 224, INSERM alliée au CNRS, Faculté de Médecine Xavier Bichaf Laboratoire de Biochimie, Paris, France.
authors: R
authors: R
authors: Vranckx
authors: L
authors: L
authors: Savu
authors: A
authors: A
authors: Cohen
authors: M
authors: M
authors: Maya
authors: E
authors: E
authors: Nunez
mesh_terms: D000209:Acute-Phase Proteins / Q000097:blood*; D000818:Animals; D002352:Carrier Proteins / Q000097:blood; D005260:Female; D005312:Fetal Blood / Q000378:metabolism; D005333:Fetus / Q000502:physiology*; D006728:Hormones / Q000097:blood; D005779:Immunodiffusion; D007123:Immunoelectrophoresis, Two-Dimensional; D007249:Inflammation / Q000097:blood* / Q000139:chemically induced; D011247:Pregnancy; D051381:Rats; D014425:Turpentine / Q000633:toxicity
chemical_list: D000209:Acute-Phase Proteins; D002352:Carrier Proteins; D006728:Hormones; D014425:Turpentine