title: [Molecular hybridization of nucleic acids as a method for the laboratory diagnosis of influenza in model experiments on cell cultures and in research on nasopharyngeal smears obtained from patients].
abstract: DNA probes containing the nucleotide sequences of the conservative genes of influenza A virus (matrix, nucleoprotein and acidic polymerase genes) show their specificity with respect to the RNA of influenza A viruses in mammal tissue cell cultures (continuous spaniel kidney cell culture and primary calf kidney cell culture). The minimal amount of infected monolayer cells, permitting the detection of viral RNA, is 10(3). The results obtained in the study of nasopharyngeal washings make it possible to recommend the method of molecular hybridization for use in the epidemiological analysis in addition to virological and serological tests. The method of hybridization permits the detection of virus-specific RNA in the allantoic fluid of chick embryos in subculturing the materials under study even in those cases when hemagglutinating influenza virus cannot be isolated.
authors: A Z
authors: AZ
authors: Pliusnin
authors: O V
authors: OV
authors: Nolandt
authors: E V
authors: EV
authors: Kuznetsova
authors: E A
authors: EA
authors: Briantseva
authors: E A
authors: EA
authors: Fridman
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D002642:Chick Embryo; D015342:DNA Probes; D004195:Disease Models, Animal; D004285:Dogs; D006801:Humans; D009980:Influenza A virus / Q000235:genetics* / Q000302:isolation & purification; D007251:Influenza, Human / Q000175:diagnosis* / Q000235:genetics / Q000382:microbiology; D008722:Methods; D009305:Nasopharynx / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*; D014776:Virus Cultivation
chemical_list: D015342:DNA Probes; D012367:RNA, Viral; D012313:RNA
vernacular_title: Molekuliarnaia gibridizatsiia nukleinovykh kislot kak metod laboratornoĭ diagnostiki grippa v model'nykh éksperimentakh na kul'turakkh kletok i pri issledovanii nosoglotochnykh smyvov, poluchennykh ot bol'nykh.