Antigen form influences induction and frequency of influenza-specific class I and class II MHC-restricted cytolytic T lymphocytes.
The induction of class I and class II MHC-restricted CTL in response to different forms of A/JAP/57 influenza virus was compared. Splenocytes removed from influenza-immune BALB/c mice and stimulated in vitro with infected syngeneic splenocytes are mainly CD8+ (Lyt-2+) and specifically lyse infected Ia- and Ia+ target cells. To a lesser extent they also lyse non-infectious virus-pulsed Ia+ but not Ia- target cells. In contrast, syngeneic stimulators pulsed with non-infectious virus (exogenous Ag) induce effector T cells that specifically lyse both infected and non-infectious virus-pulsed Ia+ target cells. The cells present in this heterogeneous culture predominantly express the CD4 (L3T4) cell surface marker. Frequency analysis by limiting dilution of splenocytes derived directly from influenza-immune mice revealed a similar pattern of precursor induction: In vitro stimulation with infected splenocytes yielded primarily class I MHC-restricted CTL, whereas stimulation with non-infectious virus reciprocally induced primarily class II MHC-restricted CTL. Thus, the Ag form and consequently the intracellular route of viral Ag presentation profoundly influence the MHC restriction of CTL precursors induced.
Morrison
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Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110.
Braciale
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Braciale
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D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002999:Clone Cells / Q000145:classification / Q000276:immunology; D000939:Epitopes / Q000502:physiology*; D005260:Female; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology*; D009980:Influenza A virus / Q000276:immunology*; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype; D013602:T-Lymphocytes, Cytotoxic / Q000145:classification* / Q000276:immunology
D000954:Antigens, Surface; D000939:Epitopes; D006183:H-2 Antigens; D000949:Histocompatibility Antigens Class II