Insulin-like growth factor I (IGF-I) enhances hyaluronic acid synthesis in rabbit pericardium.
We examined which substances stimulate hyaluronic acid synthesis in rabbit pericardium and pericardial cells. (1) The addition of 10% (v/v) pericardial fluid to the incubation medium of the pericardium enhanced hyaluronic acid synthesis to about 2.5-times over the control, suggesting that there were some stimulating factors of the hyaluronic acid synthesis in the pericardial fluid. (2) The addition of 100 ng/ml insulin-like growth factor-I (IGF-I) to the incubation medium caused an approx. 2-fold increase of hyaluronic acid synthesis over the control in the pericardium. (3) IGF-I stimulated the hyaluronic acid synthesis in pericardial cells in a dose-dependent manner, but did not significantly stimulate the sulfated glycosaminoglycan synthesis. (4) The increase in the hyaluronic acid synthesis induced by IGF-I (100 ng/ml) or pericardial fluid was blocked by the copresence of monoclonal anti-IGF-I antibody. (5) IGF-I caused possible induction of the hyaluronic acid synthase activity and general protein and RNA synthesis before the initiation of DNA synthesis in pericardial cells. These results suggest a new biological action of IGF-I that stimulates hyaluronic acid synthesis in pericardium or the pericardial cavity of heart.
Honda
A
A
Department of Biochemistry, Tokyo College of Pharmacy, Japan.
Iwai
T
T
Mori
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D002850:Chromatography, Gel; D004247:DNA / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D005122:Exudates and Transudates / Q000378:metabolism; D014453:Glucuronosyltransferase / Q000032:analysis; D006025:Glycosaminoglycans / Q000096:biosynthesis; D016695:Glycosyltransferases; D000076002:Hyaluronan Synthases; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D007334:Insulin-Like Growth Factor I / Q000276:immunology / Q000494:pharmacology*; D008565:Membrane Proteins; D010496:Pericardium / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D011402:Pronase; D012313:RNA / Q000032:analysis; D011817:Rabbits; D013002:Somatomedins / Q000494:pharmacology*; D014166:Transferases; D029867:Xenopus Proteins
D000911:Antibodies, Monoclonal; D006025:Glycosaminoglycans; D008565:Membrane Proteins; D013002:Somatomedins; D029867:Xenopus Proteins; D012313:RNA; D007334:Insulin-Like Growth Factor I; D006820:Hyaluronic Acid; D004247:DNA; D014166:Transferases; D016695:Glycosyltransferases; D014453:Glucuronosyltransferase; C056944:HAS1 protein, Xenopus; D000076002:Hyaluronan Synthases; D011402:Pronase