title: Effect of isoprinosine on lymphocyte proliferation and natural killer cell activity following thermal injury.
abstract: The effect of in vivo administration of Isoprinosine (ISO) on, i) the proliferation of splenic lymphocytes in response to the T-cell mitogen, concanavalin-A (Con-A) and, ii) the natural killer (NK) cell cytotoxicity was studied following a full skin thickness burn injury in a rat model. Administration of ISO (100 mg/kg body wt/day) twice daily, resulted in significant augmentation of the proliferative responses of lymphocytes compared to non-treated burned animals, at 7 days post injury. However, it did not effect the lymphoproliferation at 14 days post injury, the time period at which a complete suppression of lymphocyte proliferation was observed in burned non-treated animals. Also, the proliferation of lymphocytes from normal nonburned animals was not affected by treatment with ISO. ISO treatment of the burned animals resulted in a significant increase in the NK cytotoxicity compared to non-treated burned animals. As with Con-A responses, ISO administered to control nonburned animals did not have any effect on NK cell cytotoxicity. Our studies thus indicate that ISO can be a potential immunomodulator of suppressed immune function following thermal injury, particularly in patients whose lymphocyte responses to T cell mitogen Con-A are not completely suppressed.
authors: Department of Surgery, University of Texas Medical Branch, Galveston 77550.
authors: H
authors: H
authors: Singh
authors: D N
authors: DN
authors: Herndon
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D002056:Burns / Q000188:drug therapy* / Q000276:immunology; D003208:Concanavalin A / Q000494:pharmacology; D007288:Inosine / Q000031:analogs & derivatives*; D007542:Inosine Pranobex / Q000627:therapeutic use*; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
chemical_list: D000276:Adjuvants, Immunologic; D003208:Concanavalin A; D007288:Inosine; D007542:Inosine Pranobex