Selective expression of the Ly-6C.2 epitope on Lyt-2+ T cells that can develop in the absence of thymic influence.
The present study focused on attempts to correlate the expression of T cell differentiation antigens and development. A series of mAb was developed that recognized an Ly-6.2-linked Ag, Ly-6C.2. These mAb reacted with a subset of peripheral Lyt-2+ T lymphocytes but not Lyt-2+ thymocytes. Functional analysis of the Lyt-2+, Ly-6C.2- and Lyt-2+, Ly-6C.2+ subsets demonstrated that both cell types responded in class I and class II allogeneic MLC as well as in virus-specific cell-mediated cytolysis assay, although precursor frequency analysis suggested that the majority of precursor CTL were Ly-6C.2+. The marker did not appear to represent an activation Ag because Ly-6C.2-, Lyt-2+ precursor CTL generated Ly-6C.2-, Lyt-2+ CTL. Finally, virtually all splenic Lyt-2+ T cells from athymic (nu/nu) mice were Ly-6C.2+ whereas all thymic CTL were Ly-6C.2-. These results suggested that Ly-6C.2+, Lyt-2+ T cells may represent a distinct lineage of Lyt-2+ T cells that can develop in the absence of thymic influence.
Leo
O
O
Transplantation Biology Section, National Cancer Institute, Bethesda, MD 20892.
Foo
M
M
Forman
J
J
Shivakumar
S
S
Rabinowitz
R
R
Bluestone
J A
JA
D000818:Animals; D000950:Antigens, Ly / Q000032:analysis* / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000032:analysis* / Q000276:immunology; D007519:Isoantigens; D008213:Lymphocyte Activation; D051379:Mice; D008809:Mice, Inbred C3H; D008819:Mice, Nude; D010641:Phenotype; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000145:classification*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology* / Q000276:immunology; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology
D000950:Antigens, Ly; D000939:Epitopes; D007519:Isoantigens