Structures of asparagine-linked oligosaccharides of the glycoprotein fetuin having sialic acid linked to N-acetylglucosamine.
In the accompanying paper (Bendiak et al., 1989), the separation of a series of oligosaccharides released from asparagine residues of fetuin was described. A series of NMR experiments, which included one- and two-dimensional nuclear Overhauser enhancement, two-dimensional correlation spectroscopy, and two-dimensional relayed-coherence spectroscopy, as well as permethylation analyses, established a Gal beta 1----3(NeuAc alpha 2----6)GlcNAc beta 1----4Man unit common to a series of purified structures. These oligosaccharides contained either three, four, or five glycosidically linked sialic acid residues. The NeuAc residue in alpha 2----6 linkage to GlcNAc gives rise to diagnostic chemical shift perturbations of particular proton signals in the oligosaccharides.
Cumming
D A
DA
Department of Medical Genetics, University of Toronto, Ontario, Canada.
Hellerqvist
C G
CG
Harris-Brandts
M
M
Michnick
S W
SW
Carver
J P
JP
Bendiak
B
B
D000117:Acetylglucosamine; D001212:Asialoglycoproteins; D001216:Asparagine; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002849:Chromatography, Gas; D060748:Fetuins; D005944:Glucosamine / Q000031:analogs & derivatives; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000302:isolation & purification*; D000509:alpha-Fetoproteins
D001212:Asialoglycoproteins; D060748:Fetuins; D009844:Oligosaccharides; D000509:alpha-Fetoproteins; C017029:asialofetuin; D001216:Asparagine; D005944:Glucosamine; D000117:Acetylglucosamine