B lymphocyte regeneration in marrow and blood after autologous bone marrow transplantation: increased numbers of B cells carrying activation and progression markers.
The reconstitution of B cells in the bone marrow and peripheral blood was prospectively studied in 27 patients undergoing autologous bone marrow transplantation (ABMT). No major differences in B cell regeneration patterns were recorded between patients receiving marrows purged of B cells (anti-CD10 + 19; n = 17) and patients receiving unpurged marrows (n = 10). Compared with healthy controls, elevated absolute and relative numbers of B cells were recorded in the blood and marrow at +6 and +12 months in both groups of patients. CD23+ B cells were severely depressed during the first three months post ABMT, indicating immaturity. A twofold increase in B cells carrying the activation marker 4F2 was recorded in the marrow at +1 month. Serum immunoglobulin levels (IgG, IgA, IgM) were within low-normal range throughout the study. The depressed B cell responses reported after allogeneic and autologous BMT could in part be explained by the low expression of the CD23 antigen on B cells after such therapy.
Bengtsson
M
M
Department of Clinical Immunology and Transfusion Medicine, University Hospital, Uppsala, Sweden.
Smedmyr
B
B
Festin
R
R
Oberg
G
G
Simonsson
B
B
Tötterman
T H
TH
D018941:Antigens, CD19; D000944:Antigens, Differentiation, B-Lymphocyte; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000166:cytology*; D016026:Bone Marrow Transplantation / Q000473:pathology*; D002455:Cell Division; D005434:Flow Cytometry; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D008213:Lymphocyte Activation; D011446:Prospective Studies; D011961:Receptors, Fc / Q000032:analysis; D017455:Receptors, IgE; D014182:Transplantation, Autologous
D018941:Antigens, CD19; D000944:Antigens, Differentiation, B-Lymphocyte; D000954:Antigens, Surface; D007136:Immunoglobulins; D011961:Receptors, Fc; D017455:Receptors, IgE