Laboratory control of immunocytochemistry.
Monoclonal antibody technology is now used in many haematology departments for the analysis and phenotyping of haematological malignancies. Immunocytochemical analysis, in particular the alkaline phosphatase anti-alkaline phosphatase (APAAP) technique is increasing in popularity. This paper describes the findings from the 1st year of an external quality assurance scheme for immunocytochemistry. Initially, there was a wide variation amongst the results received. An improvement was seen over the year with subsequent concordance in the results. This variation could be due to a number of variables within the technique which this report highlights.
Forrest
M J
MJ
Department of Haematology, Northern General Hospital, Sheffield, U.K.
Barnett
D
D
D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis; D018941:Antigens, CD19; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry / Q000592:standards*; D007753:Laboratories / Q000592:standards*; D011786:Quality Control
D000911:Antibodies, Monoclonal; D000941:Antigens; D018941:Antigens, CD19; D000944:Antigens, Differentiation, B-Lymphocyte; D006684:HLA-DR Antigens