Expression of synthetic genes encoding bovine and human basic fibroblast growth factors (bFGFs) in Escherichia coli.
Synthetic genes encoding bovine and human basic fibroblast growth factors (bFGFs) were assembled and cloned using established Escherichia coli expression plasmids. Transformed E. coli cells were able to synthesize either a fusion protein, comprising the first seven amino acids of beta-galactosidase, a linker fragment and bovine FGF, or genomic human bFGF. The two growth factors were purified from E. coli lysates by cation exchange and heparin-Sepharose affinity chromatography. The purified recombinant proteins were biologically active as monitored by their mitogenic activity for bovine aortic endothelial cells and their angiogenic capacity in the rabbit cornea.
Knoerzer
W
W
PROGEN Biotechnik GmbH, Heidelberg, F.R.G.
Binder
H P
HP
Schneider
K
K
Gruss
P
P
McCarthy
J E
JE
Risau
W
W
D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D001483:Base Sequence; D015153:Blotting, Western; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D003001:Cloning, Molecular; D003315:Cornea / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D005346:Fibroblast Growth Factors / Q000235:genetics* / Q000302:isolation & purification; D005786:Gene Expression Regulation; D005813:Genes, Synthetic; D005822:Genetic Vectors; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000302:isolation & purification / Q000378:metabolism; D010766:Phosphorylation; D010957:Plasmids; D011817:Rabbits; D011994:Recombinant Proteins / Q000302:isolation & purification; D013194:Staining and Labeling
D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; D005346:Fibroblast Growth Factors