Isolation and characterization of the cDNA for pulmonary surfactant-associated protein-B (SP-B) in the rabbit.
Pulmonary surfactant contains phospholipids including dipalmitoyl-phosphatidylcholine and three surfactant-associated proteins designated SP-A, SP-B and SP-C. A cDNA for rabbit SP-B has been isolated from a fetal (30 days gestation) rabbit lung cDNA library constructed in lambda gt11. The cDNA and deduced amino acid sequences show strong homology with the cDNAs and predicted 40 kDa proproteins for human and canine SP-B. Strong homology is also observed with the amino acid sequences directly determined for the mature 8 kDa bovine and porcine SP-B isolated from lung lavage. SP-B is remarkable for its high cysteine and proline content and for the hydrophobic nature of the organic solvent-soluble, mature protein. In vitro translation of sense but not antisense RNA transcribed from the cDNA led to the production of 40 kDa and 32 kDa proteins. These proteins were immunoprecipitated by an antibody raised against bovine SP-B. Northern blot analysis revealed the mRNA for rabbit SP-B appears in fetal rabbit lung late in gestation and falls slightly in the neonate.
Xu
J J
JJ
Department of Obstetrics & Gynaecology, University of Western Ontario, London, Canada.
Richardson
C
C
Ford
C
C
Spencer
T
T
Yao
L J
LJ
Mackie
G
G
Hammond
G
G
Possmayer
F
F
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D004285:Dogs; D006801:Humans; D007163:Immunosorbent Techniques; D008168:Lung / Q000032:analysis / Q000196:embryology / Q000254:growth & development; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D011510:Proteolipids / Q000235:genetics*; D011663:Pulmonary Surfactants / Q000235:genetics*; D012313:RNA / Q000235:genetics; D011817:Rabbits; D012689:Sequence Homology, Nucleic Acid; D013552:Swine; D014158:Transcription, Genetic
D011510:Proteolipids; D011663:Pulmonary Surfactants; D012313:RNA; D004247:DNA