title: Prevention of lethal endotoxemia in actinomycin D-sensitized mice by incubation of Salmonella minnesota R595 lipopolysaccharide with monoclonal antibodies to R595.
abstract: Murine monoclonal antibodies reacting with lipopolysaccharide (LPS) of Salmonella minnesota strain R595 (Re chemotype) were prepared, and tested for their ability to protect actinomycin D-sensitized mice against lethal endotoxemia. Protection was found with some antibodies up to a 90-fold increase in LD50, whereas others exhibited no protection. The various protective antibodies did not all bind to the same epitope. The same applied for non-protective clones. Protective and non-protective clones could not be discriminated by ELISA. One protective monoclonal antibody (clone 20) was specific for ketodeoxyoctonate, a structural element common to various LPS. These findings show that the involvement of lipid A in the binding site of monoclonal antibodies is no prerequisite for protection.
authors: Department of Medical Microbiology, Vrije Universiteit, School of Medicine, Amsterdam, The Netherlands.
authors: B J
authors: BJ
authors: Appelmelk
authors: A M
authors: AM
authors: Verweij-van Vught
authors: H
authors: H
authors: Brade
authors: J J
authors: JJ
authors: Maaskant
authors: W F
authors: WF
authors: Schouten
authors: L G
authors: LG
authors: Thijs
authors: D M
authors: DM
authors: MacLaren
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D000936:Antigen-Antibody Complex; D003609:Dactinomycin / Q000627:therapeutic use*; D000939:Epitopes; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D012475:Salmonella / Q000276:immunology*; D014115:Toxemia / Q000517:prevention & control*
chemical_list: D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000939:Epitopes; D008070:Lipopolysaccharides; D003609:Dactinomycin