title: Reduced axonal transport of the G4 molecular form of acetylcholinesterase in the rat sciatic nerve during aging.
abstract: Aging in the sciatic nerve of the rat is characterized by various alterations, mainly cytoskeletal impairment, the presence of residual bodies and glycogen deposits, and axonal dystrophies. These alterations could form a mechanical blockade in the axoplasm and disturb the axoplasmic transports. However, morphometric studies on the fiber distribution indicate that the increase of the axoplasmic compartment during aging could obviate this mechanical blockade. Analysis of the axoplasmic transport, using acetylcholinesterase (AChE) molecular forms as markers, demonstrates a reduction in the total AChE flow rate, which is entirely accounted for by a significant bidirectional 40-60% decrease in the rapid axonal transport of the G4 molecular form. However, the slow axoplasmic flow of G1 + G2 forms, as well as the rapid transport of the A12 form of AChE, remain unchanged. Our results support the hypothesis that the alterations observed in aged nerves might be related either to the impairment in the rapid transport of specific factor(s) or to modified exchanges between rapidly transported and stationary material along the nerves, rather than to a general defect in the axonal transport mechanisms themselves.
authors: Université Catholique de Louvain, Laboratoire de Biologie Cellulaire, Louvain la Neuve, Belgium.
authors: J
authors: J
authors: Goemaere-Vanneste
authors: J Y
authors: JY
authors: Couraud
authors: R
authors: R
authors: Hassig
authors: L
authors: L
authors: Di Giamberardino
authors: P
authors: P
authors: van den Bosch de Aguilar
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000375:Aging / Q000378:metabolism; D000818:Animals; D001370:Axonal Transport; D001369:Axons / Q000648:ultrastructure; D001692:Biological Transport; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D003599:Cytoskeleton / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011919:Rats, Inbred Strains; D012584:Sciatic Nerve / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D000110:Acetylcholinesterase