Understanding Romanowsky staining. 2. The staining mechanism of suspension-fixed cells, including influences of specimen morphology on the Romanowsky-Giemsa effect.
Romanowsky staining of suspension-fixed lymphocytes and fibroblasts, deposited as monolayers on slides, involves an initial basic dyeing process followed by formation of a hydrophobic Azur B/Eosin Y complex at the more permeable and so faster staining cellular sites. This mechanism is shared with blood and marrow smears. However certain morphological features peculiar to suspension-fixed, cell culture-derived preparations also influence the staining pattern via rate control: namely the irregular and bulky profiles of fibroblasts, compared to the smoother and thinner lymphocytes; and the occasional superficial occlusion of cells by culture medium.
Horobin
R W
RW
Department of Biomedical Science, University of Sheffield, England.
Curtis
D
D
Pindar
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D001399:Azure Stains; D002478:Cells, Cultured; D004801:Eosine Yellowish-(YS); D005347:Fibroblasts / Q000032:analysis* / Q000166:cytology; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D008214:Lymphocytes / Q000032:analysis* / Q000166:cytology; D010640:Phenothiazines
D001399:Azure Stains; D010640:Phenothiazines; C046541:Romanowsky-Giemsa stain; D004801:Eosine Yellowish-(YS)