Micrococcus luteus, a bacterium with a high genomic G + C content, contains Escherichia coli-type promoters.
The G + C content of Micrococcus luteus DNA is 74%, which is much higher than that of Escherichia coli (about 50%). In order to understand the influence of GC-directed mutation pressure (GC pressure) on the promoter structure, the initiation sites for both in vitro and in vivo transcription of M. luteus streptomycin (str) and spectinomycin (spc) operons were identified by the reverse transcriptase mapping method. The promoter sequences of M. luteus are similar to those of E. coli, but have significantly higher G + C contents. In an in vitro run-off transcription assay using truncated DNA templates, RNA polymerases from both M. luteus and E. coli were able to transcribe correctly from both the str promoter and the spc major promoter of M. luteus.
Nakayama
M
M
Department of Molecular Genetics, National Institute of Genetics, Shizuoka, Japan.
Fujita
N
N
Ohama
T
T
Osawa
S
S
Ishihama
A
A
D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D008837:Micrococcus / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009876:Operon; D011401:Promoter Regions, Genetic; D012194:RNA-Directed DNA Polymerase; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D000198:Spectinomycin / Q000032:analysis; D013307:Streptomycin / Q000032:analysis; D014158:Transcription, Genetic
D000198:Spectinomycin; D012194:RNA-Directed DNA Polymerase; D013307:Streptomycin