title: Transactivating potential of the 3' open reading frame of murine mammary tumor virus.
abstract: The procaryotic chloramphenicol acetyltransferase (CAT) gene controlled by the murine mammary tumor virus (MMTV) promoter shows reduced activity in a rat mammary tumor cell line after infection with MMTV but to a considerably lesser extent than the CAT gene controlled by a heterologous promoter, indicating trans-acting regulation of promoter activity by MMTV. Cotransfection of vectors capable of expressing RNA from the 3' open reading frame (orf) of MMTV with the CAT-MMTV construct resulted in enhanced CAT activity, suggesting that orf carries a transactivating potential. Since transactivation was also found with a vector containing only orf and part of the viral env gene, it was concluded that a separate transcriptional unit exists for the orf message.
authors: Department of Retrovirology, Radiobiological Institute, Rijswijk, The Netherlands.
authors: P
authors: P
authors: van Klaveren
authors: P
authors: P
authors: Bentvelzen
mesh_terms: D000818:Animals; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D004279:DNA, Viral / Q000502:physiology*; D005786:Gene Expression Regulation; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics*; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012313:RNA / Q000096:biosynthesis; D013539:Simian virus 40 / Q000235:genetics; D013997:Time Factors; D014162:Transfection
chemical_list: D004279:DNA, Viral; D012313:RNA; D015500:Chloramphenicol O-Acetyltransferase