Effect of staurosporine on histamine release from rat serosal mast cells.
Rat serosal mast cells were challenged with compound 48/80 or calcium ionophore A23187 and the effect of staurosporine, a new inhibitor of protein kinase C, on histamine release from the cells was investigated. Histamine release induced by compound 48/80 or calcium ionophore A23187 was inhibited by staurosporine in a concentration-dependent manner and 0.1 and 1 microM staurosporine inhibited the histamine release significantly. The inhibitory effect of K-252a, another novel protein kinase C-inhibitor, was significantly higher than that of staurosporine on calcium ionophore A23187-induced histamine release. These results suggest that protein kinases will be involved in the process during mediator release from rat serosal mast cells.
Kurosawa
M
M
First Department of Internal Medicine, Gunma University School of Medicine, Maebashi, Japan.
Okayama
Y
Y
Kobayashi
S
S
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002227:Carbazoles / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects*; D026121:Indole Alkaloids; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000201:enzymology / Q000276:immunology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D051381:Rats; D011919:Rats, Inbred Strains; D012704:Serous Membrane; D019311:Staurosporine; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D000470:Alkaloids; D002227:Carbazoles; D026121:Indole Alkaloids; D000001:Calcimycin; D003189:p-Methoxy-N-methylphenethylamine; C049985:staurosporine aglycone; D011493:Protein Kinase C; D019311:Staurosporine