Flow-cytometric cell-cycle analysis of Chinese hamster cells following exposure to cytotoxicants.
Chinese hamster cell-cycle kinetics were studied following exposure to a cytotoxic agent. Different parameters like dependence on concentration and preparation interval were measured by flow cytometry. The results were compared with data from established methods. DNA histograms showed the cell-cycle effect of EMS (ethyl methanesulfonate) at different time intervals after treatment. The principle of quenching the fluorescence of Hoechst 33258 by staining 5-bromodeoxyuridine (BrdU) substituted DNA was applied and supports these results. Comparison to chromosome-aberration studies demonstrates the suitability of this method to screen quickly for adequate dosing of a cytotoxic substance and also gives information on the appropriate preparation interval.
Fertig
G
G
Institute of Zoology, Technical University, Darmstadt, F.R.G.
Miltenburger
H G
HG
D000818:Animals; D006690:Bisbenzimidazole; D001973:Bromodeoxyuridine; D002453:Cell Cycle / Q000187:drug effects*; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D005020:Ethyl Methanesulfonate / Q000494:pharmacology*; D005434:Flow Cytometry / Q000379:methods; D007700:Kinetics; D008168:Lung
D005020:Ethyl Methanesulfonate; D001973:Bromodeoxyuridine; D006690:Bisbenzimidazole