title: Ia+ epithelial dendritic cells during oral carcinogenesis in the rat.
abstract: Epithelial dendritic cells (EDC) were examined during the induction and growth of oral squamous cell carcinomas in rats treated with the carcinogen 4-Nitroquinoline N-oxide (4NQO). Acetone-fixed frozen sections of the palate and tongue were stained using an indirect immunoperoxidase technique and a monoclonal antibody to rat Ia (MRC OX-6). After 6 months there was a significant increase in Ia+ EDC/mm2 in non-invasive palatal and lingual epithelium compared with untreated and solvent painted controls. Furthermore, after 9 months there were significantly more Ia+ EDC/mm2 in non-invasive lingual epithelium compared with invasive epithelium or the epithelium overlying/adjacent to squamous cell carcinomas of the tongue. Although there were no significant differences of Ia+ EDC/mm2 between infiltrating epithelium of lingual carcinomas and non-invasive epithelium overlying/adjacent to the tumour, these tissues did contain significantly more Ia+ EDC than lingual epithelium from either solvent-only or untreated controls. The results indicate that treatment with 4NQO stimulates an increase in Ia+ EDC numbers which, although remaining higher than in controls, is not maintained within, or adjacent to, sites of neoplastic change.
authors: University Department of Oral Medicine, Surgery and Pathology, University of Bristol, England.
authors: A
authors: A
authors: Pitigala-Arachchi
authors: J B
authors: JB
authors: Matthews
authors: I J
authors: IJ
authors: Crane
authors: C
authors: C
authors: Scully
authors: S S
authors: SS
authors: Prime
mesh_terms: D015112:4-Nitroquinoline-1-oxide; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000473:pathology*; D003713:Dendritic Cells / Q000276:immunology / Q000473:pathology*; D004848:Epithelium / Q000473:pathology; D000949:Histocompatibility Antigens Class II; D007124:Immunoenzyme Techniques; D008297:Male; D009061:Mouth Mucosa / Q000473:pathology; D009062:Mouth Neoplasms / Q000139:chemically induced / Q000473:pathology*; D009600:Nitroquinolines; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling
chemical_list: D000949:Histocompatibility Antigens Class II; D009600:Nitroquinolines; D015112:4-Nitroquinoline-1-oxide