A monoclonal antibody that recognizes sialyl-Lea oligosaccharide, but is distinct from NS 19-9 as to epitope recognition.
A murine monoclonal antibody, designated as MSW 113, was generated using a human colonic cancer cell line, SW 1116, as the immunogen. MSW 113 was shown to be directed mainly to mucin-type oligosaccharide with sialyl-Lea antigens. The reactivity of MSW 113 to sialyl-Lea was stronger than that of NS 19-9, which is believed to be raised against the same determinant group. MSW 113 binds to sialyl-Lea-ol, LS-tetrasaccharide a, and disialyllacto-N-tetraose with higher affinities, compared to NS 19-9. These two antibodies could clearly be distinguished in that MSW 113 bound to sialic acid but not to fucose, whereas NS 19-9 bound to fucose but not to sialic acid. Thus, MSW 113 is directed more toward sialic acid-containing terminal structures while NS 19-9 is directed toward fucose-containing internal structures. MSW 113 was found to be useful for detecting antigens in the bloodstream of patients, especially those with pancreas cancer. Even NS 19-9 negative patient sera were positive for MSW 113.
Kitagawa
H
H
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University.
Nakada
H
H
Numata
Y
Y
Kurosaka
A
A
Fukui
S
S
Funakoshi
I
I
Kawasaki
T
T
Yamashina
I
I
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000917:Antibody Formation; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000276:immunology; D003110:Colonic Neoplasms / Q000276:immunology; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007983:Lewis Blood Group Antigens / Q000276:immunology*; D009844:Oligosaccharides / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000939:Epitopes; D007983:Lewis Blood Group Antigens; D009844:Oligosaccharides; C016735:sialooligosaccharides