Application of a silver-binding assay to the determination of protein in cerebrospinal fluid.
We evaluated a silver-binding assay for use in measuring total protein in cerebrospinal fluid. The advantage of this procedure over other methods is that, because of its sensitivity, it requires only a 0.5-microL sample. The procedure, which takes approximately 40 min to complete, involves dilution of 0.5-microL samples to 1 mL with distilled water containing sodium dodecyl sulfate, followed by addition of glutaraldehyde and an ammoniacal silver solution. After color development for 30 min, the reaction is terminated with sodium thiosulfate and the absorbance is measured at 420 nm. This assay displayed within-run and day-to-day precision (CV) of 3.1% to 13% over the range of 210 to 1370 mg/L. It showed substantially less protein-to-protein variation than the Coomassie Blue dye-binding procedure when tested with albumin, globulin, and transferrin. It also yielded an accurate estimation of hemoglobin. Moreover, preliminary studies suggested that it was capable of quantifying immunoglobulin light chains and glycoproteins. In a study of 54 human cerebrospinal fluid samples, results of the silver-binding assay corresponded more closely with those obtained with a rate biuret assay (intraclass correlation coefficient = 0.91) than did either the dye-binding or classical Lowry methods.
Krystal
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Terry Fox Laboratory, B.C. Cancer Research Centre, Vancouver, Canada.
Lam
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Schreiber
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D000418:Albumins / Q000134:cerebrospinal fluid; D001737:Biuret; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D006801:Humans; D007074:Immunoglobulin G / Q000134:cerebrospinal fluid; D008982:Molybdenum; D011485:Protein Binding; D012394:Rosaniline Dyes; D012834:Silver; D013194:Staining and Labeling; D014168:Transferrin / Q000134:cerebrospinal fluid; D014414:Tungsten; D017973:Tungsten Compounds
D000418:Albumins; D002556:Cerebrospinal Fluid Proteins; C029556:Folin's phenol reagent; D007074:Immunoglobulin G; D012394:Rosaniline Dyes; D014168:Transferrin; D017973:Tungsten Compounds; D012834:Silver; C048139:Coomassie blue; D008982:Molybdenum; D001737:Biuret; D014414:Tungsten