Common antigenicity of mouse 42 degrees C-specific heat-shock protein with mouse HSP 105.
Mammalian cells incubated at 42 degrees C synthesize a specific heat-shock protein at 42 degrees C (42 degrees C-hsp) that is not induced by heat-shock at 45 degrees C or by other stresses that induce major heat shock proteins (Hatayama et al. (1986) Biochem. Biophys. Res. Commun. 137, 957-963). Antibody raised against a heat-shock protein with molecular weight of 105,000 (hsp 105) purified from mouse FM 3A cells cross-reacted to the 42 degrees C-hsp of the same cells. The antibody reacted only weakly to hsp 105 and 42 degrees C-hsp of human HeLa cells. These results suggested that hsp 105 and 42 degrees C-hsp have the same antigenic determinant, and that 42 degrees C-hsp may have a structure similar to that of hsp 105.
Honda
K
K
Department of Biochemistry, Osaka City University Medical School, Japan.
Hatayama
T
T
Yukioka
M
M
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D000939:Epitopes / Q000032:analysis / Q000276:immunology*; D006367:HeLa Cells; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007163:Immunosorbent Techniques; D051379:Mice; D008970:Molecular Weight; D013696:Temperature
D000939:Epitopes; D006360:Heat-Shock Proteins; D007106:Immune Sera