S-protein is synthesized by human monocytes and macrophages in vitro.
Human monocytes and alveolar and peritoneal macrophages were cultured in serum-free medium with or without endotoxin (ET), agarose beads, or cycloheximide. The cell culture supernatants were collected after various intervals and examined by a monoclonal anti-S-protein antibody in Western blot and in a solid-phase enzyme immunoassay. We found that the phagocytes synthesize and secrete S-protein. ET stimulation or prolonged incubation of the cells did not favour S-protein production, which was inhibited by cycloheximide. Agarose stimulation increased the S-protein level in supernatants from monocyte but not from macrophage cultures.
Hetland
G
G
Bloodbank, Ullevål Hospital, Oslo, Norway.
Pettersen
H B
HB
Mollnes
T E
TE
Johnson
E
E
D000328:Adult; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002474:Cell-Free System; D002478:Cells, Cultured; D006023:Glycoproteins / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D008264:Macrophages / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism*; D019096:Vitronectin
D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D019096:Vitronectin