Real-time imaging of axonally transported subresolution organelles in vertebrate myelinated axons.
A procedure is described for the real-time imaging of organelles of sub-resolution dimensions that undergo rapid axonal transport in myelinated axons. The procedure uses commercially available processors to enhance images that are obtained with a video microscope. Image detail is enhanced by spatial high-pass filtering. Small moving organelles are detected by subtracting a dynamically updated image of stationary cellular detail from one that contains the same stationary features plus images of the moving organelles.
Smith
R S
RS
Department of Surgery, University of Alberta, Edmonton, Canada.
D000818:Animals; D001370:Axonal Transport; D066298:In Vitro Techniques; D009413:Nerve Fibers, Myelinated / Q000378:metabolism / Q000502:physiology*; D015388:Organelles / Q000378:metabolism / Q000502:physiology*; D011898:Ranidae; D011901:Ranvier's Nodes / Q000032:analysis; D051381:Rats; D012584:Sciatic Nerve / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D013997:Time Factors; D014741:Video Recording