[O-specific L-hapten in the composition of Shigella sonnei].
Along with classical lipopolysaccharide (LPS), O-specific material not precipitated by ultracentrifugation has been isolated from the water-phenol extract of S. sonnei avirulent strain 9090 possessing complete antigenic properties. The purification of O-antigen contained in the supernatant fluid has been carried out by the gel filtration of the fluid, previously treated with ribonuclease, in a column packed with Sephadex G-100. The polysaccharide nature of O-antigen thus obtained, the absence of lipid A and KDO and the low content of hexoses, or core-specific saccharides of S. sonnei LPS, in this antigen make it possible to classify this material with O-components of microbial cells, described by different authors as "native protoplasmic polysaccharide" or "L-hapten" and formed by polymers of LPS O-side chains. The content of this component in S. sonnei strains under study is, on the average, 2.5% of the weight of dry microbial substance. L-hapten preparations obtained in the course of our investigations have been found to contain two O-specific antigens detected by immunoelectrophoresis and immunodiffusion, as well as by sedimentation in saccharose gradient, where they form peaks corresponding to 4.3 S and 10.8 S. This polysaccharide O-antigen is supposed to be capable of interaction with ribosomal particles and suitable for use as a component of ribosomal dysentery vaccines.
Egorova
T P
TP
Sazykina
S Iu
SIu
Fedosova
V G
VG
Lebenson
V I
VI
D000942:Antigens, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D001428:Bacterial Vaccines / Q000276:immunology; D000939:Epitopes / Q000032:analysis / Q000302:isolation & purification*; D006241:Haptens / Q000032:analysis / Q000302:isolation & purification*; D008070:Lipopolysaccharides / Q000032:analysis / Q000302:isolation & purification; D019081:O Antigens; D012270:Ribosomes / Q000276:immunology; D012764:Shigella sonnei / Q000032:analysis / Q000276:immunology* / Q000472:pathogenicity; D014774:Virulence / Q000276:immunology
D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D000939:Epitopes; D006241:Haptens; D008070:Lipopolysaccharides; D019081:O Antigens
O-spetsificheskiĭ L-gapten v sostave Shigella sonnei.