[Simultaneous photometry of the DNA and silver-grain count in liver cells labelled with 3H-thymidine or 3H-leucine].
A modified method was proposed for reflected light simultaneous measurement of DNA content and of the silver grain number in the nucleus or cytoplasm of the same cell. Specimens-smears of isolated liver cells incorporated 3H-thymidine and 3H-leucine were prepared on coverslips and after processing were mounted on the slide glasses with smeared side facing downwards to avoid the influence of grains on DNA content measurements. To decrease the background, label measurements were carried out in polarized light. It was shown that the intensity of 3H-leucine incorporation in hepatocytes increases proportionally with cell ploidy degree.
Shteĭn
G I
GI
Kudriavtsev
B N
BN
D000818:Animals; D001345:Autoradiography; D002467:Cell Nucleus / Q000032:analysis; D003592:Cytophotometry / Q000295:instrumentation / Q000379:methods; D003593:Cytoplasm / Q000032:analysis; D004247:DNA / Q000032:analysis*; D005374:Filtration / Q000295:instrumentation; D007930:Leucine; D008099:Liver / Q000032:analysis*; D008297:Male; D051379:Mice; D011003:Ploidies; D051381:Rats; D012834:Silver / Q000032:analysis*; D013194:Staining and Labeling / Q000379:methods; D013936:Thymidine; D014316:Tritium
D014316:Tritium; D012834:Silver; D004247:DNA; D007930:Leucine; D013936:Thymidine
Odnovremennaia fotometriia DNK i chisla zeren serebra v kletkakh pecheni, mechennykh 3H-timidinom ili 3H-leĭtsinom.