title: [A method of determining the concentration of thiamine].
abstract: The rate of the production of thiazole, the first product of the reaction, catalyzed by thiaminase I (EC 2.5.1.2) from Anodonta cygnea muscles is characterized by an initial flash-up explained by a considerable difference in the rates of the enzyme-substrate complex formation and its further transformation. Basing on this phenomenon, a new method for measuring the total thiamine in vitro and in biological material has been developed. Optimal conditions for thiamine measurements in the blood and urine have been selected.
authors: S S
authors: SS
authors: Puzach
authors: Z V
authors: ZV
authors: Gorbach
mesh_terms: D000818:Animals; D066298:In Vitro Techniques; D008722:Methods; D051381:Rats; D013831:Thiamine / Q000032:analysis* / Q000097:blood / Q000652:urine
chemical_list: D013831:Thiamine
vernacular_title: Metod opredeleniia kontsentratsii tiamina.