title: Distribution of desmoplakin in normal cultured human keratinocytes and in basal cell carcinoma cells.
abstract: In cultured human keratinocytes (NHEK) maintained in medium containing low levels of Ca2+ (0.04 mM) desmoplakin is a component of certain electron-dense bodies in the cytoplasm. These bodies are associated with bundles of intermediate filaments. Upon elevation of the level of Ca2+ in the culture medium to 1.2 mM, desmoplakin first appears at sites of cell-cell contact in association with bundles of intermediate filaments. Subsequently, desmoplakin becomes incorporated into desmosomes in a manner comparable to that seen in mouse keratinocytes (Jones and Goldman: Journal of Cell Biology 101:506-517, 1985). NHEK cells maintained for 24 hr at Ca2+ concentrations between 0.04 mM and 0.18 mM were processed for immunofluorescence, immunoelectron, and conventional electron microscopical analysis. In NHEK cells grown at Ca2+ concentrations of 0.11 mM, desmoplakin appears to be localized in electron-dense bodies associated with intermediate filaments at sites of cell-cell contact in the absence of formed desmosomes. At a Ca2+ concentration of 0.13 mM desmoplakin is arrayed like beads on a "string" of intermediate filaments at areas of cell-cell association. At 0.15 mM, desmosome formation occurs, and desmoplakin is associated with the desmosomal plaque. In basal cell carcinoma cells desmoplakin is not restricted to desmosomes but also occurs in certain electron-dense bodies morphologically similar to those seen in NHEK maintained in low levels of Ca2+ and during early stages of desmosome assembly. We discuss the possibility of "cycling" of desmoplakin through these bodies in proliferative cells.
authors: Department of Cell Biology and Anatomy, Northwestern University Medical School, Chicago, IL 60611.
authors: J C
authors: JC
authors: Jones
authors: K A
authors: KA
authors: Grelling
mesh_terms: D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D002280:Basal Cell Carcinoma / Q000032:analysis* / Q000648:ultrastructure; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000032:analysis*; D051180:Desmoplakins; D003896:Desmosomes / Q000032:analysis*; D004817:Epidermis / Q000032:analysis* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006801:Humans; D007382:Intermediate Filaments / Q000032:analysis; D007633:Keratins; D008854:Microscopy, Electron; D014407:Tumor Cells, Cultured
chemical_list: D003598:Cytoskeletal Proteins; C495294:DSP protein, human; D051180:Desmoplakins; D007633:Keratins; D002118:Calcium