Transplantation of major AB0-incompatible bone marrow: removal of red cells by dextrane sedimentation.
The elimination of erythrocytes by dextran sedimentation is a useful method to prevent haemolytic complications after AB0-incompatible bone marrow transplantation. The procedure presented here lasts only 1.5-2 hours, is high reproducible and safe in respect of infections. The mean recovery of nucleated cells amounted to 94%. The contamination with erythrocytes is low (recovery 1.1%). We have never seen haemolytic reactions during 7 AB0-incompatible bone marrow transplantations.
Müller
A
A
Universitäts-Kinderklinik und Poliklinik Jussuf Ibrahim, Jena.
Hermann
J
J
Fuchs
D
D
Prager
J
J
Burger
E
E
Kob
D
D
Goetz
I
I
Zintl
F
F
D000017:ABO Blood-Group System / Q000276:immunology; D000906:Antibodies / Q000032:analysis; D001787:Blood Group Incompatibility; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation / Q000276:immunology*; D002469:Cell Separation / Q000379:methods*; D002648:Child; D003911:Dextrans; D004912:Erythrocytes / Q000166:cytology* / Q000276:immunology; D006801:Humans
D000017:ABO Blood-Group System; D000906:Antibodies; D003911:Dextrans