title: Inflammatory cells degrade inter-alpha inhibitor to liberate urinary proteinase inhibitors.
abstract: The relationship between inter-alpha inhibitor (I alpha I) and urinary proteinase inhibitor (UPI) was examined by comparing purified UPI with a proteolytic fragment of I alpha I (I'), and by demonstrating that inflammatory cells produce similar fragments under physiologic conditions. Purified I', derived by chymotrypsin digestion of I alpha I, was similar to UPI in apparent molecular weight (68,000-69,000), amino acid composition, immunoreactivity, and inhibitory activity against trypsin, chymotrypsin, and neutrophil elastase. The production of similar inhibitory fragments by murine peritoneal macrophages, human neutrophils, and a murine mast cell line was quantified. Neutrophils were most efficient at proteolyzing I alpha I. Comparison of the pattern of I alpha I degradation by neutrophil preparations with that by pure enzymes, suggested that both elastase and cathepsin G mediate neutrophil proteolysis of I alpha I. These proteinases may thus be responsible for inflammation-related increases in UPI-like inhibitor levels in vivo.
authors: Department of Biochemistry, Duke University Medical Center, Durham, NC 27710.
authors: C W
authors: CW
authors: Pratt
authors: M W
authors: MW
authors: Swaim
authors: S V
authors: SV
authors: Pizzo
mesh_terms: D000510:Alpha-Globulins / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D008264:Macrophages / Q000378:metabolism*; D008407:Mast Cells / Q000378:metabolism*; D051379:Mice; D009504:Neutrophils / Q000378:metabolism*
chemical_list: D000510:Alpha-Globulins; D006023:Glycoproteins; C011877:inter-alpha-inhibitor; C028665:urinastatin