Helper-independent retrovirus vectors with Rous-associated virus type O long terminal repeats.
We have constructed nonpermuted replication-competent avian retrovirus vectors that derive from Rous sarcoma virus (S. H. Hughes, J. J. Greenhouse, C. J. Petropoulos, and P. Sutrave, J. Virol. 61:3004-3012, 1987). We describe here the construction and properties of corresponding vectors in which the long terminal repeats (LTRs) of the parental virus have been replaced by the LTRs of the endogenous chicken virus Rous-associated virus type O. The Rous-associated virus type O LTR vectors replicated approximately 1/10 as well as the parental vectors and expressed a test gene, chloramphenicol acetyltransferase, approximately 1/30 to 1/50 as well.
Greenhouse
J J
JJ
National Cancer Institute-Frederick Cancer Research Facility, Maryland 21701-1013.
Petropoulos
C J
CJ
Crittenden
L B
LB
Hughes
S H
SH
D000818:Animals; D001354:Avian Leukosis Virus / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D002642:Chick Embryo; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000235:genetics; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics*; D005347:Fibroblasts; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010957:Plasmids; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid; D014162:Transfection; D014779:Virus Replication
D015342:DNA Probes; D004279:DNA, Viral; D015500:Chloramphenicol O-Acetyltransferase; D012194:RNA-Directed DNA Polymerase