Parallel DNA: generation of a duplex between two Drosophila sequences in vitro.
We have observed the existence of a parallel complementary region between two Drosophila DNA sequences, fragments of the suffix [(1986) EMBO J, 5, 2341-2347] and a 5'-non-coding sequence of the alcohol dehydrogenase gene [(1983) Cell 33, 125-133]. The region includes approximately 40 bp, 76% of which are complementary in the same polarity. Synthetic complementary 16 bp oligonucleotides corresponding to this region which were bound by the 5'-ends through a 1.6-hexanediol bridge form a duplex which displays both melting and annealing as judged by UV absorbance. Anti parallel complementary 16 bp long oligonucleotides bound by the 5'-3' ends through the same bridge and a single-strand sequence were used as controls. The Hoechst 33,258 drug binds to this parallel duplex of DNA; however, the properties of such a complex testify against the B-form of the duplex.
Tchurikov
N A
NA
Department of Nucleic Acids Biosynthesis, Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
Chernov
B K
BK
Golova
Y B
YB
Nechipurenko
Y D
YD
D000426:Alcohol Dehydrogenase / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D002942:Circular Dichroism; D004247:DNA / Q000648:ultrastructure*; D004251:DNA Transposable Elements; D004331:Drosophila melanogaster / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D012313:RNA
D004251:DNA Transposable Elements; D012313:RNA; D004247:DNA; D000426:Alcohol Dehydrogenase