Epitopes of the Mycobacterium tuberculosis 65-kilodalton protein antigen as recognized by human T cells.
A synthetic peptide approach has been used to identify the epitopes recognized by clonal and polyclonal human T cells reactive to the recombinant mycobacterial 65-kDa protein Ag. Three of the four epitopes identified were recognized as cross-reactive between Mycobacterium tuberculosis and Mycobacterium leprae, although their amino acid sequence in two of three cases was not identical. The peptide (231-245) defining an epitope recognized as specific to the M. tuberculosis complex contains two substitutions compared with the homologous M. leprae region of which one or both are critical to T cell recognition. The reactive T cell clones showed helper/inducer phenotype (CD4+, CD8-), and secrete IL-2, granulocyte-macrophage-CSF, and IFN-gamma upon Ag stimulation. The same clones display cytotoxicity against macrophages pulsed with the relevant peptides or mycobacteria.
Oftung
F
F
Laboratory for Immunology, Norwegian Radium Hospital, Oslo.
Mustafa
A S
AS
Shinnick
T M
TM
Houghten
R A
RA
Kvalheim
G
G
Degre
M
M
Lundin
K E
KE
Godal
T
T
D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000302:isolation & purification*; D002999:Clone Cells / Q000276:immunology / Q000378:metabolism; D000939:Epitopes / Q000302:isolation & purification*; D006801:Humans; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000096:biosynthesis; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology; D010449:Peptide Mapping; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
D000942:Antigens, Bacterial; D000939:Epitopes; D008222:Lymphokines; D010446:Peptide Fragments