Ontogenic expression of the two isozymes, Ch1 and Ch2, of chicken muscle acylphosphatase.
Activities of the two isozymes, Ch1 and Ch2, of chicken muscle acylphosphatase were measured in breast muscles, leg muscles, and livers of developing chicks from day 11 in ovo to day 15 of free life. Measurement was performed using rabbit antibodies which could selectively precipitate Ch1 or Ch2. The activity contents of both Ch1 and Ch2 in muscles were low before hatching but rapidly increased after hatching. Ch1 showed a more marked increase than Ch2. In liver, on the other hand, the activity contents of Ch1 and Ch2 remained low throughout the period of pre- and post-hatching.
Ohba
Y
Y
Section of Biochemistry, Hokkaido University.
Mizuno
Y
Y
Shiokawa
H
H
D017766:Acid Anhydride Hydrolases; D000818:Animals; D000918:Antibody Specificity; D002645:Chickens; D004531:Eggs; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007074:Immunoglobulin G / Q000032:analysis; D007527:Isoenzymes; D008099:Liver / Q000201:enzymology; D008297:Male; D024510:Muscle Development; D009132:Muscles / Q000201:enzymology*; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D013194:Staining and Labeling; D014018:Tissue Distribution; D000097024:Acylphosphatase
D017766:Acid Anhydride Hydrolases; D007074:Immunoglobulin G; D007527:Isoenzymes; D010744:Phosphoric Monoester Hydrolases; D000097024:Acylphosphatase