Immunohistological study of Crooke's cells.
By immunohistochemical methods, 28 postmortem pituitary glands with unequivocal Crooke's hyaline change were examined to identify the hyaline material. Crooke's hyaline was positive for 55-57 kilodalton (KD) cytokeratin (CK) but negative for 68 KD CK, vimentin, desmin, neurofilament and glial fibrillary acidic protein. Involucrin, a new marker for keratinocyte differentiation, could also not be demonstrated. It was concluded that Crooke's hyaline was an abnormally accumulated CK which is a normal constituent of the ACTH cell.
Uei
Y
Y
Cytology Section, Clinical Laboratory, National Cancer Center Hospital, Japan.
D000911:Antibodies, Monoclonal; D003366:Cosyntropin / Q000032:analysis; D003893:Desmin / Q000032:analysis; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D007381:Intermediate Filament Proteins / Q000032:analysis; D007633:Keratins / Q000032:analysis; D016900:Neurofilament Proteins; D010902:Pituitary Gland / Q000166:cytology / Q000473:pathology*; D014746:Vimentin / Q000032:analysis
D000911:Antibodies, Monoclonal; D003893:Desmin; D005904:Glial Fibrillary Acidic Protein; D007381:Intermediate Filament Proteins; D016900:Neurofilament Proteins; D014746:Vimentin; D003366:Cosyntropin; D007633:Keratins