Identification of differentiation-specific antigens in psoriatic versus normal skin using monoclonal antibodies.
Human cell hybrids derived from fusions between carcinoma (HeLa) and normal epidermal keratinocytes can be used as immunogens to generate monoclonal antibodies against keratinocyte differentiation-specific antigens. We examined the immunofluorescent staining patterns of these monoclonals in normal human and psoriatic (lesional and uninvolved) skin tissue sections. The immunofluorescent staining patterns indicate that the monoclonal antibodies are recognizing a number of different keratinocyte antigens. Three monoclonals (HLK7, HLK3, and HLK20) displayed different immunofluorescent reactivity in these tissues. These new monoclonal antibodies will be useful tools to study the differentiation abnormalities in psoriasis and may serve as a marker of the psoriatic diathesis.
McCullough
J L
JL
Department of Dermatology, University of California, College of Medicine, Irvine 92717.
Weinstein
G D
GD
Ross
P
P
Barr
R J
RJ
Stanbridge
E J
EJ
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000032:analysis*; D001706:Biopsy; D002478:Cells, Cultured; D000078404:Epidermal Cells; D005455:Fluorescent Antibody Technique; D006801:Humans; D007633:Keratins / Q000276:immunology*; D011565:Psoriasis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D007633:Keratins