Human cytotoxic T-cell recognition of a synthetic peptide of myelin basic protein.
Previous studies with a panel of myelin basic protein (MBP)-specific human T-cell clones suggested a clustering of epitopes in the middle and at the C terminus of the molecule. The current study demonstrates that 19 of 40 clones recognize a synthetic peptide corresponding to residues 152 to 170 of the human MBP molecule and that 9 clones recognize a synthetic peptide corresponding to residues 86 to 105. Myelin basic protein-specific cytotoxic activity was restricted to the clones that recognized peptide 152-170, and this peptide served as a preferential cytotoxic T-cell target when attached to an autologous B-cell line. The specificity of MBP-directed cytotoxic activity appears to be much more restricted than the specificity demonstrated for proliferative activity.
Richert
J R
JR
Department of Neurology, Georgetown University Medical Center, Washington, DC 20007.
Robinson
E D
ED
Deibler
G E
GE
Martenson
R E
RE
Dragovic
L J
LJ
Kies
M W
MW
D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes; D006801:Humans; D066298:In Vitro Techniques; D004676:Myelin Basic Protein / Q000138:chemical synthesis / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000939:Epitopes; D004676:Myelin Basic Protein