Intrinsic properties of neurones in the dorsal cochlear nucleus of mice, in vitro.
1. Intracellular recordings were made from the dorsal cochlear nucleus (DCN) in slices of the cochlear nuclear complex. Probably the larger and most frequent cells were impaled. 2. The steady-state current-voltage (I-V) properties of all cells impaled were nonlinear. The I-V curve was steepest in the voltage range depolarized from the resting potential and most shallow when the cell was hyperpolarized from rest by more than about 10 mV. Thus, the inwardly rectifying I-V characteristics of cells in the DCN distinguish them from those of ventral cochlear nuclear neurones (Oertel, 1983). 3. When depolarized with current, most cells fired trains of large, all-or-none action potentials. The undershoot after single spikes comprised an initial, fast component followed by a second, slower wave. A few cells (15%) generated bursts of smaller, graded spikes in addition to the large ones. 4. Repetitive firing evoked by depolarizing pulses of current was followed by an after-hyperpolarization whose magnitude depended on the strength and duration of the preceding current pulse. 5. Blocking the large action potentials with tetrodotoxin (TTX) revealed Ca2+-dependent spikes in all cells examined. 6. The steady-state I-V relationship became linear in the presence of TTX, suggesting that a persistent Na+ conductance probably mediates the inward rectification seen above the resting potential. 7. Muscarine at micromolar concentrations excited cells and increased their input resistance.
Hirsch
J A
JA
Department of Neurophysiology, University of Wisconsin, Madison 53706.
Oertel
D
D
D015761:4-Aminopyridine; D000200:Action Potentials / Q000187:drug effects; D000631:Aminopyridines / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000502:physiology*; D002118:Calcium / Q000502:physiology; D003056:Cochlear Nerve / Q000502:physiology*; D066298:In Vitro Techniques; D007473:Ion Channels / Q000502:physiology; D051379:Mice; D009116:Muscarine / Q000494:pharmacology; D009474:Neurons / Q000502:physiology*; D012964:Sodium / Q000502:physiology; D019789:Tetraethylammonium; D013757:Tetraethylammonium Compounds / Q000494:pharmacology; D013779:Tetrodotoxin / Q000494:pharmacology; D013997:Time Factors
D000631:Aminopyridines; D007473:Ion Channels; D013757:Tetraethylammonium Compounds; D013779:Tetrodotoxin; D019789:Tetraethylammonium; D009116:Muscarine; D012964:Sodium; D015761:4-Aminopyridine; D002118:Calcium