In vitro functional blocking of myelin basic protein-specific cytolytic human T lymphocyte clones by immunosuppressive drugs and monoclonal antibodies.
The in vitro effects of cyclosporin A, prednisolone, and anti-CD4 monoclonal antibody WW.T4 on myelin basic protein-specific human CD4+ cytolytic T lymphocyte clones were studied. Functional assays of antigen-specific proliferation, induction of specific lysis, cytolysis itself, and interferon-gamma production were done. Prednisolone decreased secretion of interferon-gamma by the clones and blocked specific proliferation; the latter could, however, be overcome by the addition of exogenous interleukin 2. It did not influence cytolytic properties. In contrast, cyclosporin A and WW.T4 blocked the four antigen-specific functions of the autoimmune myelin basic protein-specific human T cell clones measured.
Weber
W E
WE
Department of Immunology, Dr. L. Willems Institute, Diepenbeek, Belgium.
Buurman
W A
WA
D000911:Antibodies, Monoclonal / Q000276:immunology*; D002999:Clone Cells; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007371:Interferon-gamma / Q000096:biosynthesis; D008213:Lymphocyte Activation; D004676:Myelin Basic Protein / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology* / Q000502:physiology
D000911:Antibodies, Monoclonal; D007166:Immunosuppressive Agents; D004676:Myelin Basic Protein; D007371:Interferon-gamma