Mouse and human CD14 (myeloid cell-specific leucine-rich glycoprotein) primary structure deduced from cDNA clones.
cDNA clones complementary to MS7-4 (Setoguchi et al. (1988) Somat. Cell Mol. Genet. 14, 427-438) from a mouse macrophage cDNA library were separated. Sequence analysis of these clones demonstrated that the longest cDNA clone, MS7X, had a 1366 bp insert and high homology with that of the human CD14 gene (Ferrero and Goyert (1988) Nucleic Acids Res. 16, 4173). Using the MS7X cDNA probe, cDNA clones were separated from cDNA libraries constructed from a human macrophage cell line and macrophages. The total cDNA sequence was 1364 bp in length, with an open reading frame of 1125 nucleotides matching that of the human CD14 gene except for one nucleotide difference. The amino-acid sequence (mouse CD14), deduced from the nucleotide sequence of the MS7X insert consisted of 351 amino-acid residues with a high leucine content (17.66%) and five putative N-glycosylation sites, and in vitro translation predicted a protein of molecular mass of 37.5 kDa. Human CD14 had 356 amino-acid residues, with high leucine content (15.5%), and contained four putative N-glycosylation sites. Mouse CD14 showed 13 building blocks, of which internal nine blocks have a conserved leucine motif and significant homology with human leucine-rich alpha 2-glycoprotein.
Setoguchi
M
M
Department of Pathology, Medical College of Oita, Japan.
Nasu
N
N
Yoshida
S
S
Higuchi
Y
Y
Akizuki
S
S
Yamamoto
S
S
D000595:Amino Acid Sequence; D000818:Animals; D015214:Antigens, Differentiation, Myelomonocytic / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D004247:DNA; D006023:Glycoproteins / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells; D018950:Lipopolysaccharide Receptors; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000235:genetics; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
D015214:Antigens, Differentiation, Myelomonocytic; D006023:Glycoproteins; C015246:LRG1 protein, human; D018950:Lipopolysaccharide Receptors; D021382:Protein Sorting Signals; D004247:DNA