Growth inhibition of Epstein-Barr virus-transformed B cells by anti-HLA-DR antibody L243: possible relationship to L243-induced down-regulation of CD23 antigen expression.
Among five anti-HLA class II monoclonal antibodies (mab's) tested, the anti-HLA-DR mab L243 selectively inhibited the in vitro proliferation of Epstein-Barr virus-transformed lymphoblastoid cell lines (LCL). Saturating amounts of L243 mab induced 50% suppression of LCL growth as revealed by measuring [3H]thymidine incorporation or counting cell numbers. Preincubation for 20 hr at 37 degrees C in the presence of L243 mab dramatically reduced the expression of certain B cell-specific antigens on LCL, notably CD23 (BLAST-2, low affinity Fc epsilon receptor). In view of the known function of the CD23 antigen as a B cell growth factor receptor, we discuss the possibility that the suppressive effect of L243 mab on LCL proliferation is due to down-regulation of CD23 antigen expression.
Kabelitz
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Department of Medical Microbiology and Immunology, University of Ulm, Federal Republic of Germany.
Janssen
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D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D018951:Antigens, CD20; D000944:Antigens, Differentiation, B-Lymphocyte / Q000502:physiology*; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D002455:Cell Division; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D006684:HLA-DR Antigens / Q000276:immunology*; D004854:Herpesvirus 4, Human; D011961:Receptors, Fc / Q000502:physiology*; D017455:Receptors, IgE
D000911:Antibodies, Monoclonal; D018951:Antigens, CD20; D000944:Antigens, Differentiation, B-Lymphocyte; D000954:Antigens, Surface; D006684:HLA-DR Antigens; D011961:Receptors, Fc; D017455:Receptors, IgE