title: Ultrastructure of the absorptive cell glycocalyx in hyperplastic colonic polyps after staining with alcian blue and high iron diamine.
abstract: The glycocalyx of absorptive cells in large intestinal hyperplastic polyp was characterized histochemically at the electron microscope level by the use of the Alcian Blue pH 2.5 and high iron diamine techniques with the aim of comparing their ability in preserving the fine reticular network of the structure. Both the reagents stained glycocalyx, indicating the presence of sulphated acidic glycoconjugates. However, they showed different degrees of condensation of the reactive sites. Alcian Blue preserved its filamentous appearance better.
authors: Laboratorio di Ultrastructture, Istituto Superiore di Sanità, Rome, Italy.
authors: F M
authors: FM
authors: Albedi
authors: F
authors: F
authors: Ciaralli
authors: D
authors: D
authors: Taruscio
authors: A
authors: A
authors: Giuliani
mesh_terms: D000423:Alcian Blue; D003111:Colonic Polyps / Q000648:ultrastructure*; D003959:Diamines; D006651:Histocytochemistry; D006801:Humans; D006965:Hyperplasia / Q000473:pathology; D007501:Iron; D008854:Microscopy, Electron; D013194:Staining and Labeling
chemical_list: D003959:Diamines; D007501:Iron; D000423:Alcian Blue