Interleukin-6 and interleukin-1 production in acute leukemia with monocytoid differentiation.
Several authors have reported the in vitro production of colony-stimulating factors (CSF) and interleukin-1 (IL-1) by the neoplastic cells from patients with acute myeloid leukemia (AML). Using a sensitive bioassay for IL-6, the capacity of the leukemic cells of 30 patients with AML to produce IL-6 was examined. IL-6 production was found to be specific for cells from patients with an AML with monocytic differentiation (12 of 15 M4 and M5 patients, 0 of 15 M1 and M2 patients). Moreover, IL-6 production was paralleled by IL-1 production. The IL-6- and IL-1-producing cells were mainly found in the more mature monocytic cell fractions, defined as CD14-positive and CD34-negative adherent cells. By limiting dilution experiments, it could be excluded that the production of IL-1 or IL-6 was due to contamination with normal monocytes.
van der Schoot
C E
CE
Central Laboratory, The Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Jansen
P
P
Poorter
M
M
Wester
M R
MR
von dem Borne
A E
AE
Aarden
L A
LA
van Oers
R H
RH
D018952:Antigens, CD34; D000943:Antigens, Differentiation; D015214:Antigens, Differentiation, Myelomonocytic; D015152:Blotting, Northern; D002448:Cell Adhesion; D002454:Cell Differentiation; D006801:Humans; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000096:biosynthesis*; D015850:Interleukin-6 / Q000096:biosynthesis* / Q000235:genetics; D007948:Leukemia, Monocytic, Acute / Q000378:metabolism*; D015479:Leukemia, Myelomonocytic, Acute / Q000378:metabolism*; D018950:Lipopolysaccharide Receptors; D012333:RNA, Messenger / Q000235:genetics
D018952:Antigens, CD34; D000943:Antigens, Differentiation; D015214:Antigens, Differentiation, Myelomonocytic; D007375:Interleukin-1; D015850:Interleukin-6; D018950:Lipopolysaccharide Receptors; D012333:RNA, Messenger