High antibody levels against mouse laminin with specificity for galactosyl-(alpha 1-3)galactose in patients with inner ear diseases.
Sera of 413 patients with inner ear diseases were examined for ELISA binding to mouse laminin and compared to a normal group. Strongly enhanced antilaminin antibody reactions were observed in a large number of patients with sensorineural hearing loss (68%), tinnitus (60%), and sudden deafness (46%), but not in those with Meniere's disease (14%) or normal individuals (8%). Absorption experiments demonstrated that these antibodies are also responsible for the antisarcolemmal antibody pattern in the immunofluorescence test and for the reaction with pig kidney microsomes in the ELISA. Immunochemical studies showed that laminin binding of high or low titer in sera from patients and normal individuals is due to the Gal alpha 1-3Gal epitope present in N-linked oligosaccharides of mouse laminin. No reactions were observed with human laminin, which lacks this epitope. The findings suggest that high levels of antibodies against carbohydrate structures are triggered by persistent infections, providing some new insights about the possible cause of these diseases.
Klein
R
R
Department of Internal Medicine, University of Tübingen, Federal Republic of Germany.
Timpl
R
R
Zanetti
F R
FR
Plester
D
D
Berg
P A
PA
D000328:Adult; D000818:Animals; D000918:Antibody Specificity; D004187:Disaccharides / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D015151:Immunoblotting; D007759:Labyrinth Diseases / Q000378:metabolism*; D007797:Laminin / Q000276:immunology*; D008297:Male; D051379:Mice; D011863:Radioimmunoassay
D004187:Disaccharides; D000939:Epitopes; D007797:Laminin; C055075:galactosyl-(1-3)galactose