Modification of a trypsin-detergent method for DNA flow cytometry of human epidermis.
An existing technique (Vindelov et al. Cytometry 3:323, 1983) has been modified for DNA flow cytometry of human epidermis obtained from 2 to 3 mm punch biopsies. By varying the length of time of digestion of the epidermal disc by trypsin from 5 to 70 min a controlled release of keratinocytes occurred beginning with the stratum basale and proceeding toward, but not including, the superficial layer of the epidermis, the stratum corneum.
Stough
D B
DB
Department of Dermatology, University of Arkansas for Medical Sciences, Little Rock 72205.
Burns
E R
ER
Mallory
S B
SB
Pipkin
J L
JL
Hinson
W G
WG
D002450:Cell Communication / Q000187:drug effects; D002467:Cell Nucleus / Q000032:analysis / Q000648:ultrastructure; D004247:DNA / Q000032:analysis*; D003902:Detergents; D000078404:Epidermal Cells; D004817:Epidermis / Q000032:analysis* / Q000648:ultrastructure; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007633:Keratins / Q000032:analysis; D008722:Methods; D013501:Surface-Active Agents; D014357:Trypsin / Q000494:pharmacology
D003902:Detergents; D013501:Surface-Active Agents; D007633:Keratins; D004247:DNA; D014357:Trypsin