title: An antigenic profile of Lewy bodies: immunocytochemical indication for protein phosphorylation and ubiquitination.
abstract: An antigenic profile of subcortical and cortical Lewy bodies was determined in the presence or absence of neurofibrillary tangles in the same brain using antisera and monoclonal antibodies to various cytoskeletal elements as well as to determinants not present in the normal cytoskeleton. The cores of many Lewy bodies were strongly reactive with a monoclonal antibody to paired helical filaments which has been shown to recognize ubiquitin. This antibody also stained Marinesco bodies in the same tissue sections. Two monoclonal antibodies to phosphorylated epitopes of neurofilament proteins (SM I 31, SM I 34) stained the peripheries of about 40% of all discernable Lewy bodies on untreated paraffin sections. Reactivity with a monoclonal antibody to neurofilaments (SM I 33) appeared only after pretreatment of the sections with phosphatase. Lewy bodies did not bind antibodies to tau protein. Our results show that, as previously shown for neurofibrillary tangles, Lewy bodies also contain ubiquitin. The uncovering of neurofilament epitopes by treatment with phosphatase indicates that abnormal phosphorylation of cytoskeletal elements may play a role in the pathogenesis of the Lewy body.
authors: Neurological Institute, University of Vienna, Wien, Austria.
authors: C
authors: C
authors: Bancher
authors: H
authors: H
authors: Lassmann
authors: H
authors: H
authors: Budka
authors: K
authors: K
authors: Jellinger
authors: I
authors: I
authors: Grundke-Iqbal
authors: K
authors: K
authors: Iqbal
authors: G
authors: G
authors: Wiche
authors: F
authors: F
authors: Seitelberger
authors: H M
authors: HM
authors: Wisniewski
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000276:immunology*; D003599:Cytoskeleton / Q000276:immunology / Q000378:metabolism; D000939:Epitopes; D006801:Humans; D007150:Immunohistochemistry; D002479:Inclusion Bodies / Q000276:immunology* / Q000648:ultrastructure; D007382:Intermediate Filaments / Q000276:immunology; D008854:Microscopy, Electron; D008869:Microtubule-Associated Proteins / Q000276:immunology; D008875:Middle Aged; D009454:Neurofibrils / Q000276:immunology; D009474:Neurons / Q000276:immunology* / Q000378:metabolism / Q000648:ultrastructure; D010766:Phosphorylation; D011506:Proteins / Q000378:metabolism*; D014452:Ubiquitins / Q000276:immunology / Q000378:metabolism*; D016875:tau Proteins
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D000939:Epitopes; D008869:Microtubule-Associated Proteins; D011506:Proteins; D014452:Ubiquitins; D016875:tau Proteins