The role of a template sugar-phosphate backbone in the ribosomal decoding mechanism. Comparative study of poly(U) and poly(dT) template activity.
To study the role of a template sugar-phosphate backbone in the ribosomal decoding process, poly(U), poly(dT) and poly(dU)-directed cell-free amino acid incorporation was investigated under the influence of neomycin and high concentrations of Mg2+. The specificity of a factor-dependent translation system of Escherichia coli was shown to change according to the principle: "either ribo- or deoxyribopolynucleotide messenger". Poly(dT) is shown to be effectively translated in the absence of elongation factors, both at low (2 degrees C) and high (37 degrees C) temperature. Neomycin inhibits factor-free poly(dT) translation. Little or no poly(U) translation is observed in this system. A chromatographic analysis of the oligophenylalanine residues synthesized seems to show that translocation is the main step responsible for ribosome specificity to the ribo- or deoxyribopolynucleotide template in both factor-dependent and factor-free translation systems.
Potapov
A P
AP
Institute of Molecular Biology and Genetics, Ukrainian SSR Academy of Sciences, Kiev, U.S.S.R.
Soldatkin
K A
KA
Soldatkin
A P
AP
El'skaya
A V
AV
D002474:Cell-Free System; D004926:Escherichia coli; D007700:Kinetics; D010452:Peptide Biosynthesis; D010455:Peptides; D011071:Poly T / Q000235:genetics*; D011072:Poly U / Q000235:genetics*; D011089:Polydeoxyribonucleotides / Q000235:genetics*; D014176:Protein Biosynthesis; D012329:RNA, Bacterial / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012345:RNA, Transfer, Amino Acid-Specific / Q000235:genetics*; D012360:RNA, Transfer, Phe / Q000235:genetics*; D012270:Ribosomes
D010455:Peptides; D011089:Polydeoxyribonucleotides; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012345:RNA, Transfer, Amino Acid-Specific; D012360:RNA, Transfer, Phe; D011071:Poly T; D011072:Poly U; C012001:polyphenylalanine