title: Acute effect of cigarette smoke on cytoplasmic motility of alveolar macrophages in dogs.
abstract: To study effects of cigarette smoke on the cytoplasmic motility (CM) of alveolar macrophages (AM), we measured remanent field strength (RFS) in dogs in vivo. Four days after instillation of ferrimagnetic particles (Fe3O4, 3 mg/kg) into the right lower lobe bronchus, RFS was measured at the body surface immediately after magnetization of the Fe3O4 particles by an externally applied magnetic field. RFS decreased with time due to particle rotation (relaxation), which is thought to be inversely related to CM of AM (J. Appl. Physiol. 55: 1196-1202, 1983). The initial relaxation curve was fitted to an exponential function. The relaxation rate (lambda 0) increased during cigarette smoke inhalation and returned to base-line values within 15 min. With the inhalation of the smoke of up to five cigarettes, peak lambda 0 was increased; with a further increase in the number of cigarettes, the effect of cigarette smoke decreased or disappeared. Nicotine injection and acetylcholine inhalation increased respiratory resistance to a degree similar to that observed with cigarette smoke but did not change lambda 0. However, either substance P (SP) or capsaicin injection increased lambda 0 in a fashion similar to that noted with cigarette smoke inhalation. Repeated administration of SP produced a significant tachyphylaxis of the effect, and capsaicin did not increase lambda 0 after the cigarette smoke-induced tachyphylaxis of the effect. Colchicine inhibited the cigarette smoke-induced increase in lambda 0. These results suggest that cigarette smoke increases CM of AM, probably through the release of tachykinins including SP from sensory nerves in the lung.
authors: First Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
authors: M
authors: M
authors: Yamaya
authors: K
authors: K
authors: Zayasu
authors: K
authors: K
authors: Sekizawa
authors: K
authors: K
authors: Yamauchi
authors: S
authors: S
authors: Shimura
authors: H
authors: H
authors: Sasaki
authors: T
authors: T
authors: Takishima
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002211:Capsaicin / Q000494:pharmacology; D003593:Cytoplasm / Q000502:physiology; D004285:Dogs; D052203:Ferrosoferric Oxide; D007501:Iron; D008264:Macrophages / Q000187:drug effects / Q000502:physiology*; D008280:Magnetics; D009538:Nicotine / Q000494:pharmacology; D010087:Oxides; D012016:Reference Values; D012119:Respiration / Q000187:drug effects*; D012907:Smoking / Q000503:physiopathology*; D013373:Substance P / Q000494:pharmacology
chemical_list: D010087:Oxides; D013373:Substance P; D009538:Nicotine; D007501:Iron; D000109:Acetylcholine; D002211:Capsaicin; D052203:Ferrosoferric Oxide