Lamin B shares a number of distinct epitopes with lamins A and C and with intermediate filament proteins.
Four monoclonal antibodies raised against rat liver nuclear lamins and an anti-intermediate filament antibody [Pruss, R. M., Mirsky, R., & Raff, M. C. (1981) Cell (Cambridge, Mass.) 27, 419-428] have been used to identify epitopes shared by lamin B with lamins A and C, and with intermediate filament proteins. The antibodies defined two major antigenic regions on the three lamins which were both homologous with mouse epidermal keratins as well as hamster vimentin and desmin. Three distinguishable epitopes shared by lamin B with lamins A and C were identified by competition studies between pairs of antibodies and by reaction against N-chlorosuccinimide and cyanogen bromide cleavage fragments. These results support the hypothesis that lamin B, despite important biochemical differences with lamins A and C, shares with them some of the structural characteristics typical of intermediate filament proteins.
Raymond
Y
Y
Institut du Cancer de Montréal, Hôpital Notre-Dame, Québec, Canada.
Gagnon
G
G
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000648:ultrastructure; D003599:Cytoskeleton / Q000648:ultrastructure; D000939:Epitopes / Q000032:analysis*; D007381:Intermediate Filament Proteins / Q000276:immunology*; D034921:Lamin Type B; D034882:Lamins; D008099:Liver / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D009687:Nuclear Proteins / Q000276:immunology*; D051381:Rats
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000939:Epitopes; D007381:Intermediate Filament Proteins; D034921:Lamin Type B; D034882:Lamins; D009687:Nuclear Proteins