The effect of misoprostol on base-line and stimulated acid secretion and on gastrin and histamine release in the totally isolated, vascularly perfused rat stomach.
The effects of the prostaglandin E1 analogue misoprostol on base-line and stimulated (gastrin, histamine, and the muscarinic M1 agonist McN-A-343) acid secretion, base-line and gastrin-stimulated histamine release, and base-line and McN-A-343-stimulated gastrin release in the totally isolated, vascularly perfused rat stomach were studied. At a concentration of 1 nM, misoprostol significantly (p less than 0.01) inhibited base-line acid secretion from 13.7 +/- 2.7 to 4.8 +/- 0.7 mumol/60 min, histamine-stimulated acid secretion from 99.9 +/- 15.1 to 21.8 +/- 8.2 mumol/60 min, maximal gastrin-stimulated secretion from 92.5 +/- 11.4 to 3.1 +/- 0.6 mumol/60 min, and maximal McN-A-343-stimulated secretion from 60.0 +/- 8.9 to 6.8 +/- 2.6 mumol/60 min (mean +/- SEM). Likewise, misoprostol significantly inhibited base-line vascular histamine release (p less than 0.05) from 10.1 +/- 2.3 to 3.7 +/- 0.7 nmol/60 min and gastrin-stimulated release (p less than 0.01) from 54.7 +/- 7.9 to 20.2 +/- 4.1 nmol/60 min (mean +/- SEM). The gastrin release was not affected by misoprostol. We conclude that misoprostol inhibits acid secretion both by a direct effect on the parietal cell and by inhibiting endogenous histamine release.
Sandvik
A K
AK
Dept. of Medicine, Trondheim University, Norway.
Waldum
H L
HL
D008455:(4-(m-Chlorophenylcarbamoyloxy)-2-butynyl)trimethylammonium Chloride / Q000494:pharmacology; D000527:Alprostadil / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D005744:Gastric Acid / Q000378:metabolism*; D005755:Gastrins / Q000378:metabolism* / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects*; D066298:In Vitro Techniques; D016595:Misoprostol; D010477:Perfusion; D051381:Rats; D011976:Receptors, Muscarinic / Q000187:drug effects; D013270:Stomach / Q000502:physiology
D000897:Anti-Ulcer Agents; D005755:Gastrins; D011976:Receptors, Muscarinic; D016595:Misoprostol; D008455:(4-(m-Chlorophenylcarbamoyloxy)-2-butynyl)trimethylammonium Chloride; D000527:Alprostadil