title: A novel monoclonal antibody, 1B3.1, binds to a new epitope of the VLA-1 molecule.
abstract: A monoclonal antibody, 1B3.1, was raised against a cloned IL-2-dependent T cell line that expresses the T gamma delta T cell receptor. MoAb 1B3.1 reacted with long-term cultured T cell lines of both T gamma delta and T alpha beta lineage, and with in vivo-stimulated T cells, derived from synovial fluid, but not with resting or short-term activated T cells, B cells, or macrophages. Immunoprecipitation of the 1B3.1 target antigens showed that 1B3.1 recognizes a 200/110 kDa molecule that is identical to the VLA-1 heterodimer precipitated by MoAb TS2/7. 1B3.1, however, binds to an epitope of VLA-1 that is distinct from the TS2/7 binding site. This new MoAb could be useful in further studies of the functions of VLA-1, and of the cells that express this molecule.
authors: Department of Internal Medicine and Oncology, Chaim Sheba Medical Center Tel Hashomer, Israel.
authors: I
authors: I
authors: Bank
authors: M
authors: M
authors: Hemler
authors: M B
authors: MB
authors: Brenner
authors: D
authors: D
authors: Cohen
authors: V
authors: V
authors: Levy
authors: J
authors: J
authors: Belko
authors: C
authors: C
authors: Crouse
authors: L
authors: L
authors: Chess
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D000943:Antigens, Differentiation / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology* / Q000378:metabolism; D015815:Cell Adhesion Molecules; D000939:Epitopes; D005434:Flow Cytometry; D006801:Humans; D011233:Precipitin Tests; D011948:Receptors, Antigen, T-Cell / Q000032:analysis; D016692:Receptors, Antigen, T-Cell, gamma-delta; D016029:Receptors, Very Late Antigen; D013601:T-Lymphocytes / Q000276:immunology*; D014018:Tissue Distribution
chemical_list: D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D000939:Epitopes; D011948:Receptors, Antigen, T-Cell; D016692:Receptors, Antigen, T-Cell, gamma-delta; D016029:Receptors, Very Late Antigen