Perfusion method for preparing pig brain cortex for Golgi-Cox impregnation.
The perfusion procedure described in this paper produces high quality impregnation of pig visual and somatosensory cortical neurons with a Golgi-Cox solution. Starting within 30 min after death, pig heads were perfused with a fixative solution composed of a mixture (v/v) of liquid phenol, 5%; formalin, 14%; ethylene glycol, 25%; methanol, 28%; and water, 28% for two periods of 4 hr each. After perfusion, the heads were chilled for at least 18 hr. The entire brain was removed from the skull and then placed in 10% buffered formalin, where it remained for at least 10 days before taking the blocks that were to be immersed in the Golgi-Cox solution. Three weeks spent in the Golgi-Cox solution typically produced uniform neuron impregnation. The tissue blocks were then embedded in celloidin and sectioned at 120 micron. This procedure avoids the following difficulties: Golgi-Cox methods that produced excellent results with rodent or primate tissue were unsuccessful with pig tissue, placing fresh tissue in Golgi-Cox solution resulted in incomplete neuron impregnation, and immersion fixation in 10% buffered formalin without perfusion resulted in excessive staining of glia.
Grandin
T
T
Department of Animal Sciences, University of Illinois at Urbana-Champaign 61801.
Demotte
O D
OD
Greenough
W T
WT
Curtis
S E
SE
D000818:Animals; D002540:Cerebral Cortex / Q000032:analysis*; D005404:Fixatives; D010477:Perfusion / Q000379:methods*; D012834:Silver; D013194:Staining and Labeling / Q000379:methods*; D013552:Swine
D005404:Fixatives; D012834:Silver