[Conformational changes in the chromatin of tumor cells and the phenomenon of nuclear achromasia].
Tumour cell nuclei display achromasia in smears relative to methylene blue and to other low-affinitive ionic dyes. In the course of the smear air-drying the tumour DNA undergoes conformational changes as evidenced by enhanced ethidium and monomer acridine orange uptake and increased sensitivity to DNAse I hydrolysis. Very mild treatment with bleomycin prevents nuclear achromasia with methylene blue in tumour cells. It is concluded that the phenomenon of nuclear achromasia of tumour cells is due to the properties of secondary structure of their DNA.
Erenpreĭsa
E A
EA
Sondore
O Iu
OIu
Zirne
R A
RA
D000818:Animals; D002843:Chromatin / Q000187:drug effects / Q000648:ultrastructure*; D004273:DNA, Neoplasm / Q000187:drug effects / Q000648:ultrastructure; D005354:Fibrosarcoma / Q000648:ultrastructure*; D006801:Humans; D008175:Lung Neoplasms / Q000648:ultrastructure*; D008854:Microscopy, Electron; D009374:Neoplasms, Experimental / Q000648:ultrastructure*; D009690:Nucleic Acid Conformation / Q000187:drug effects; D011487:Protein Conformation / Q000187:drug effects; D013194:Staining and Labeling / Q000379:methods; D014407:Tumor Cells, Cultured
D002843:Chromatin; D004273:DNA, Neoplasm
Konformatsionnye izmeneniia khromatina opukholevykh kletok i fenomen iadernoĭ akhromazii.