title: Partial characterization of a thyroid-stimulating hormone-like peptide in neuroendocrine cells of the human prostate gland.
abstract: Immunohistochemical identification of the most prevalent type of neuroendocrine (NE) cells in the human prostate gland can be made with polyclonal antisera against human thyroid-stimulating hormone (TSH). A TSH-like peptide was characterized by analysis of prostatic tissue homogenates with sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) electrophoresis followed by immunoblotting. A single protein band, with an apparent mass of about 32 kDa after reduction, was identified both with polyclonal antisera against human TSH and with a polyclonal antiserum raised against a synthetic peptide corresponding to the carboxyterminal part of the beta-subunit of human TSH. The TSH-like prostatic peptide identified here is, on the basis of its molecular mass and absence of immunoreactivity with an antiserum raised against a synthetic peptide representing the mid-portion of the beta-subunit of TSH, not identical with the pituitary beta-subunit of TSH. On the other hand, this 32 kDa prostatic peptide may have certain structural elements in common with the pituitary beta-subunit of TSH, since it is recognized both with polyclonal antisera against TSH and with an antiserum against the carboxyterminal part of the beta-subunit of TSH.
authors: Department of Urology, Lund University, Malmö General Hospital, Sweden.
authors: P A
authors: PA
authors: Abrahamsson
authors: H
authors: H
authors: Lilja
mesh_terms: D000368:Aged; D004848:Epithelium / Q000032:analysis / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008722:Methods; D008875:Middle Aged; D008970:Molecular Weight; D009490:Neurosecretory Systems / Q000032:analysis / Q000378:metabolism*; D010455:Peptides / Q000032:analysis / Q000378:metabolism*; D011467:Prostate / Q000032:analysis / Q000378:metabolism*; D011470:Prostatic Hyperplasia / Q000378:metabolism; D013329:Structure-Activity Relationship; D013972:Thyrotropin / Q000032:analysis / Q000378:metabolism*
chemical_list: D010455:Peptides; D013972:Thyrotropin