Purification and characterization of an endogenous inhibitor of the sialyltransferase CMP-N-acetylneuraminate: lactosylceramide alpha 2,6-N-acetylneuraminyltransferase (EC 2.4.99.-).
The presence in the 100,000 g supernatant of rat brain homogenate of an inhibitor of the sialyltransferase has been confirmed. It is also present in chicken and bovine brain and in other rat and bovine organs. The inhibitor has been purified, a preparation with a specific activity 130-fold higher than that of the original 100,000 g supernatant of brain being obtained. It runs as a single peak in polyacrylamide-gel electrophoresis; when run in the presence of SDS, two components appeared. The apparent Mr of the components were 14,800 and 22,400. The inhibitor has been characterized as a heat-stable protein of acidic nature. It has effect on the glycolipid and the glycoprotein sialyltransferase activities but has no effect on the galactosaminyltransferase activity.
Albarracin
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Departamento de Química Biológica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina.
Lassaga
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Caputto
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D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001212:Asialoglycoproteins; D001921:Brain / Q000201:enzymology; D002417:Cattle; D002645:Chickens; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002918:Chymotrypsin / Q000494:pharmacology; D060748:Fetuins; D007700:Kinetics; D008970:Molecular Weight; D051381:Rats; D012799:Sialyltransferases / Q000037:antagonists & inhibitors*; D013045:Species Specificity; D013696:Temperature; D014018:Tissue Distribution; D014357:Trypsin / Q000494:pharmacology; D000509:alpha-Fetoproteins / Q000494:pharmacology; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
D000596:Amino Acids; D001212:Asialoglycoproteins; D060748:Fetuins; D000509:alpha-Fetoproteins; C017029:asialofetuin; D012799:Sialyltransferases; D002918:Chymotrypsin; D014357:Trypsin; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase