title: Selective localization of factor VIII antigenicity to islet endothelial cells and expression of class II antigens by normal human pancreatic ductal epithelium.
abstract: We evaluated the expression of factor VIII and class II histocompatibility antigens on frozen sections of normal human and rat pancreases. The immunohistologic studies were performed with directly fluoresceinated anti-human factor VIII and monoclonal antibodies A2B5, 3G5 (anti-islet), L-243, I-2, OK1 (anti-human Ia-DR), Leu-10 (anti-human HLA-DQ), anti-human HLA-DP, and OX6 (anti-rat Ia). Islet endothelial cells of humans and Wistar, CD, and BB diabetes-prone rats could be distinguished from intra-acinar endothelial cells by markedly enhanced factor VIII immunoreactivity. Factor VIII-antibody staining of islet endothelial cells was specifically absorbed by prior incubation of anti-human factor VIII antibody with normal human plasma but not by incubation with factor VIII-deficient plasma. By double indirect immunofluorescence, normal human pancreatic ductal epithelium expressed Ia in five of six pancreases studied.
authors: Joslin Diabetes Center, Boston, MA 02215.
authors: S A
authors: SA
authors: Dib
authors: P
authors: P
authors: Vardi
authors: S
authors: S
authors: Bonner-Weir
authors: G S
authors: GS
authors: Eisenbarth
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis*; D000939:Epitopes / Q000276:immunology; D005169:Factor VIII / Q000032:analysis / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006649:Histocompatibility Antigens / Q000032:analysis*; D006801:Humans; D007223:Infant; D007515:Islets of Langerhans / Q000276:immunology; D008297:Male; D010179:Pancreas / Q000276:immunology*; D051381:Rats; D014841:von Willebrand Factor
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D000939:Epitopes; D006649:Histocompatibility Antigens; D014841:von Willebrand Factor; D005169:Factor VIII