Interferon (IFN)-like antiviral effect is induced by unspecific cross-linking of cell surface receptors.
Treatment of human amniotic cells (UAC) with Cytodex 1 (DEAE-dextran) results in the development of an antiviral state of the cells, as proven by studying (i) the cytopathic effect and (ii) [3H]uridine incorporation into the RNA of vesicular stomatitis virus (VSV) after VSV infection. The same treatment transiently triggers the breakdown of inositol phospholipids and activates the translocation of protein kinase C (PKC). On the basis of these data it can be suggested that cross-linking of cell surface receptors by a solid carrier bearing covalently bound positive charges may result in IFN-like effects.
Premecz
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G
Microbiological Research Group, National Institute of Hygiene, Budapest, Hungary.
Markovits
A
A
Bagi
G
G
Farkas
T
T
Földes
I
I
D000998:Antiviral Agents; D002460:Cell Line; D003911:Dextrans / Q000494:pharmacology*; D006801:Humans; D007295:Inositol Phosphates / Q000378:metabolism; D007372:Interferons / Q000502:physiology; D008563:Membrane Lipids / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D012367:RNA, Viral / Q000187:drug effects; D011971:Receptors, Immunologic / Q000187:drug effects* / Q000502:physiology; D017471:Receptors, Interferon; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects / Q000276:immunology; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D003911:Dextrans; D007295:Inositol Phosphates; D008563:Membrane Lipids; D012367:RNA, Viral; D011971:Receptors, Immunologic; D017471:Receptors, Interferon; C052839:Cytodex; D007372:Interferons; D011493:Protein Kinase C