title: On the role of the Shine-Dalgarno sequence in determining the efficiency of translation initiation at a weak start codon in the car operon of Escherichia coli K12.
abstract: Translation of the carA gene is efficiently initiated at the intrinsically weak UUG codon. A single nucleotide substitution changing the Shine-Dalgarno box of carA (GGAGG) into the sequence TGAGG reduces translation of carA sevenfold. This result supports the view that extensive complementarity between the Shine-Dalgarno sequence and 16S RNA contributes significantly to the efficiency of translation when the latter starts at a weak initiation triplet.
authors: Erfelijkheidsleer en Microbiologie, Vrije Universiteit Brussel, Belgium.
authors: G
authors: G
authors: Weyens
authors: D
authors: D
authors: Charlier
authors: M
authors: M
authors: Roovers
authors: A
authors: A
authors: Piérard
authors: N
authors: N
authors: Glansdorff
mesh_terms: D001483:Base Sequence; D003062:Codon; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D007763:Lac Operon; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D012329:RNA, Bacterial / Q000235:genetics; D012333:RNA, Messenger; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D003062:Codon; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012336:RNA, Ribosomal, 16S