Simple staining for heat-fixed brain sections after topographical pH analysis or autoradiographic studies.
Powerful topographic techniques are now available, among which autoradiographic and fluorescent mapping are the most prevalent. These techniques produce images that usually do not correlate with brain anatomy; subsequent staining is required to allow a precise association between the parameter(s) investigated and brain structures. A simple staining procedure is described, compatible with heat-fixed brain sections, and that was found particularly valuable in association with topographical tissue pH studies using the fluorescent pH indicator umbelliferone.
Parkinson
N A
NA
Gough-Cooper Department of Neurological Surgery, Institute of Neurology, London, England.
Christofi
G
G
Obrenovitch
T P
TP
Symon
L
L
D001345:Autoradiography; D001921:Brain / Q000033:anatomy & histology*; D006863:Hydrogen-Ion Concentration; D007211:Indoles; D013194:Staining and Labeling
D007211:Indoles; C018588:Luxol Fast Blue MBS