Antibodies to Campylobacter flagellin recognize epitopes common to phase 1 and phase 2 flagella.
We used a simple method to obtain purified flagellin from Campylobacter, suitable for an immunization procedure in mice. Western blot analysis of cross-reacting antibodies showed that there were epitopes common to phase 1 and 2 flagellins. Analysis by ELISA suggested that certain common flagellar epitopes are conformational, and antibody immobilization tests confirmed that common surface-exposed epitopes exist in a region of flagella necessary for conferring motility to the bacteria.
Kirimat
M
M
Institut Pasteur, Bangui.
Georges-Courbot
M C
MC
Georges
A J
AJ
Martin
P M
PM
D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology*; D015153:Blotting, Western; D002168:Campylobacter fetus / Q000276:immunology*; D002465:Cell Movement; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D005407:Flagella / Q000276:immunology*; D005408:Flagellin / Q000276:immunology*; D051379:Mice
D001426:Bacterial Proteins; D000939:Epitopes; D005408:Flagellin