The immunoregulatory granulocyte factor (GF). I. Some antigenic and structure features.
Adherent PMNLs secrete protein factor termed granulocyte factor (GF). The antigenic and some structural features of GF were examined in the present paper. Molecular weight of GF was established on Sephadex G-75 column and is amounted to 40 kD. GF exhibited two isoelectric points in pH ranged from 4.7 to 4.9 and from 5.7 to 6.4. SDS polyacrylamide gel electrophoresis has displayed two bands, 20 and 68 kD, respectively. Initial analysis of amino acid composition of purified GF has shown 15 amino acids, but without aromatic and thioamino acids. Antigen-dependent species specificity was confirmed using rabbit anti-human, -murine, -guinea pig or-rat - GF IgG applying conventional precipitation methods in agar gel no crossreaction was noted. Using precipitation analyses GF was localized in the specific granules of PMNLs.
Baj
Z
Z
Department of Pathophysiology, Institute of Basic Medical Sciences WAM, Lódź.
Tchórzewski
H
H
Majewska
E
E
D000954:Antigens, Surface / Q000302:isolation & purification*; D003115:Colony-Stimulating Factors / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D016179:Granulocyte Colony-Stimulating Factor; D006801:Humans; D007519:Isoantigens / Q000302:isolation & purification; D007959:Lymphocyte Culture Test, Mixed; D009504:Neutrophils / Q000276:immunology*
D000954:Antigens, Surface; D003115:Colony-Stimulating Factors; D007519:Isoantigens; D016179:Granulocyte Colony-Stimulating Factor