Diffusion of fluorescein-labelled molecules in suspensions of erythrocyte ghosts.
The diffusion of fluorescein isothiocyanate-labelled dextran molecules in suspensions of centrifugally, tightly packed, erythrocyte ghosts was measured by fluorescence recovery after photobleaching. In comparison with diffusion in aqueous solution, the diffusion coefficients for probe molecules of varying size were about two orders of magnitude smaller. It was established that the dextran molecules remained in the space between the ghosts. Since crosslinking membrane surface carbohydrates with antibodies further inhibits diffusion, it is assumed that interactions between surface carbohydrates and the probe molecules are the cause of slow diffusion. Two alternative models are discussed.
Järnefelt
J
J
Department of Medical Physics, Karolinska Institutet, Stockholm, Sweden.
Laurent
T
T
Rigler
R
R
D000906:Antibodies; D001789:Blood Group Antigens / Q000276:immunology; D002241:Carbohydrates / Q000097:blood*; D003911:Dextrans / Q000097:blood*; D004058:Diffusion; D004910:Erythrocyte Membrane / Q000378:metabolism*; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives*; D005452:Fluoresceins / Q000097:blood*; D005456:Fluorescent Dyes; D006801:Humans; D010777:Photochemistry
D000906:Antibodies; D001789:Blood Group Antigens; D002241:Carbohydrates; D003911:Dextrans; D005452:Fluoresceins; D005456:Fluorescent Dyes; C015219:fluorescein isothiocyanate dextran; C024098:6-carboxyfluorescein; D016650:Fluorescein-5-isothiocyanate