T-immunogenic peptides are constituted of rare sequence patterns. Use in the identification of T epitopes in the human immunodeficiency virus gag protein.
The sequences of a set of 63 peptides of demonstrated T immunogenicity have been analyzed and compared with two different randomly generated sets of sequences. This study indicates a statistically significant tendency of T immunogenic peptides to be constituted of clusters of rare tetrapeptides, as evaluated from the available sequence data banks. This result has been used to locate potential T epitopes in the human immunodeficiency virus (HIV) gag protein. Four peptides corresponding to the best candidate T epitopes (chosen in regions of conserved sequence among different virus isolates) have been synthesized and found to be recognized by a HIV-1-specific, HLA-A2-restricted human cytotoxic T cell line.
Claverie
J M
JM
Unité d'Informatique Scientifique, Institut Pasteur, Paris, France.
Kourilsky
P
P
Langlade-Demoyen
P
P
Chalufour-Prochnicka
A
A
Dadaglio
G
G
Tekaia
F
F
Plata
F
F
Bougueleret
L
L
D000595:Amino Acid Sequence; D000939:Epitopes; D015683:Gene Products, gag; D006678:HIV / Q000276:immunology*; D015488:HIV Antigens / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007256:Information Systems; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology; D012191:Retroviridae Proteins / Q000235:genetics / Q000276:immunology*; D013223:Statistics as Topic; D013329:Structure-Activity Relationship; D013601:T-Lymphocytes / Q000276:immunology*
D000939:Epitopes; D015683:Gene Products, gag; D015488:HIV Antigens; D010455:Peptides; D012191:Retroviridae Proteins