title: In situ hybridization technique using an immunogold silver staining system.
abstract: An in situ hybridization technique using an immunogold silver staining detection system was used to detect biotinylated DNA probes in cultured cells and in formalin-fixed paraffin-embedded tissue. The detection method is rapid, reliable and economical, producing an insoluble signal at the site of hybridization which is visible at low-power light microscopy and which can be enhanced by epipolarization microscopy.
authors: Department of Pathology, University of Leeds, UK.
authors: P
authors: P
authors: Jackson
authors: F A
authors: FA
authors: Lewis
authors: M
authors: M
authors: Wells
mesh_terms: D002460:Cell Line; D004247:DNA / Q000032:analysis*; D015342:DNA Probes; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D009693:Nucleic Acid Hybridization; D012834:Silver; D013194:Staining and Labeling
chemical_list: D015342:DNA Probes; D012834:Silver; D004247:DNA