Effect of hydrocortisone on biosynthesis of mitochondrial and cytoplasmatic RNA in liver of adrenalectomized rats.
The effect of hydrocortisone on the biosynthesis of mitochondrial and cytoplasmic RNA in the liver of adrenalectomized rats was studied. A biphasic effect of hydrocortisone on the induction of RNA biosynthesis was observed: a short-term increase at 10th and 90th minute and more prolonged increase with a maximum at 210 min. At the same the intramitochondrial pool of labelled precursors of RNA did not change. According to hybridization data the expression of mitochondrial URF 5 and 6 genes linked to the effect of glucocorticoids was selectively increased already at the initial stage of hormone effect, but the resulting remarkable increase of RNA biosynthesis did not further result in changes of the spectrum of mitochondrial RNA. Specific activity of cytoplasmic RNA was decreasing at 10 min and increasing at 1, 2 and 4 h after the administration of hydrocortisone. The size of cytoplasmic acid soluble pool of labelled RNA precursors was increased only at 4 h after the administration of the hormone. It seems that a decrease of specific activity of cytoplasmic RNA during the initial period of hormone action may result from the changes of RNA transport from the nuclei to the cytoplasm.
Minchenko
A G
AG
Laboratory of Hormonal Regulation of Metabolism, Institute of Endocrinology and Metabolism, Kiev, Ukrainian SSR.
D000315:Adrenalectomy; D000818:Animals; D003593:Cytoplasm / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D006854:Hydrocortisone / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D009963:Orotic Acid / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D014158:Transcription, Genetic
D009963:Orotic Acid; D012313:RNA; D006854:Hydrocortisone