Identification of Moraxella bovis by using a monoclonal antibody to a lipopolysaccharide epitope.
A monoclonal antibody to the lipopolysaccharide of Moraxella bovis is described. In an indirect fluorescent-antibody test, this monoclonal antibody reacted with 39 of 39 strains of M. bovis tested and did not react with 26 nonfermenting gram-negative coccobacilli other than M. bovis. When used in an indirect fluorescent-antibody test, it proved useful for rapid and easy identification of M. bovis.
Wannemuehler
Y
Y
Department of Veterinary Microbiology, Iowa State University, Ames 50010.
Johansen
K
K
Rosenbusch
R
R
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000032:analysis* / Q000276:immunology; D015153:Blotting, Western; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006825:Hybridomas; D008070:Lipopolysaccharides / Q000276:immunology; D009016:Moraxella / Q000276:immunology / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes; D008070:Lipopolysaccharides