Influence of aging on the histamine release and membrane fluidity of rat peritoneal mast cells.
The maturational changes in the degree of homologous passive cutaneous anaphylaxis (PCA) and the histamine release from peritoneal mast cells induced by several secretagogues were studied using Wistar rats (4-40 weeks old). Although the increase in vascular permeability of the rat skin induced by intradermal injection of histamine did not change significantly from one maturation period to the next, 6- to 8-weeks old rats were both the most susceptible to PCA reactions and the most responsive to histamine-releasing stimuli. Among rats in this age group (6-8 weeks), the fluidity of the resting cell membrane and the extent of membrane fluidity increase in response to compound 48/80 were greatest. Analysis of the lipid composition of mast cells indicated that the ratio of cholesterol to phospholipids was lowest at the age of 6-8 weeks. From the present study, we concluded that the maturational changes in the extent of histamine release seem to be related to membrane fluidity, which has a profile similar to that of maturation.
Mio
M
M
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Akahori
H
H
Sugimoto
Y
Y
Akagi
M
M
Tasaka
K
K
D000375:Aging / Q000378:metabolism*; D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D004396:Coloring Agents; D006632:Histamine / Q000494:pharmacology; D006636:Histamine Release; D007271:Injections, Intradermal; D050356:Lipid Metabolism; D008297:Male; D008407:Mast Cells / Q000502:physiology*; D008560:Membrane Fluidity / Q000187:drug effects*; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D004396:Coloring Agents; D010743:Phospholipids; D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine; D002784:Cholesterol