A simple metabolic system for selection of hybrid hybridomas (tetradomas) producing bispecific monoclonal antibodies.
A metabolic selective system has been proposed for the selection of hybrid hybridomas (tetradomas) based on the introduction in one of the parental cell lines of two traits simultaneously--a recessive one (resistance to 8-azaguanine) and a dominant one (multidrug resistance). Tetradomas were selected in the presence of two selective agents: aminopterin and actinomycin D. Using this approach we produced tetradomas secreting bispecific MAbs to horseradish peroxidase and human alpha-fetoprotein.
Chervonsky
A V
AV
Laboratory of Immunochemistry, Cancer Research Center, USSR Academy of Medical Sciences, Moscow.
Faerman
A I
AI
Evdonina
L V
LV
Jazova
A K
AK
Kazarov
A R
AR
Gussev
A I
AI
D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D015139:Blotting, Southern; D002460:Cell Line; D004351:Drug Resistance / Q000235:genetics; D006735:Horseradish Peroxidase / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D000509:alpha-Fetoproteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000509:alpha-Fetoproteins; D006735:Horseradish Peroxidase