Transforming growth factor-beta 1: histochemical localization with antibodies to different epitopes.
We have localized transforming growth factor-beta (TGF-beta) in many cells and tissues with immunohistochemical methods, using two polyclonal antisera raised to different synthetic preparations of a peptide corresponding to the amino-terminal 30 amino acids of TGF-beta 1. These two antibodies give distinct staining patterns; the staining by anti-CC(1-30) is intracellular. This differential staining pattern is consistently observed in several systems, including cultured tumor cells; mouse embryonic, neonatal, and adult tissues; bovine fibropapillomas; and human colon carcinomas. The extracellular staining by anti-CC(1-30) partially resembles that seen with an antibody to fibronectin, suggesting that extracellular TGF-beta may be bound to matrix proteins. The intracellular staining by anti-LC(1-30) is similar to that seen with two other antibodies raised to peptides corresponding to either amino acids 266-278 of the TGF-beta 1 precursor sequence or to amino acids 50-75 of mature TGF-beta 1, suggesting that anti-LC(1-30) stains sites of TGF-beta synthesis. Results from RIA and ELISAs indicate that anti-LC(1-30) and anti-CC(1-30) recognize different epitopes of this peptide and of TGF-beta 1 itself.
Flanders
K C
KC
Laboratory of Chemoprevention, National Cancer Institute, Bethesda, Maryland 20892.
Thompson
N L
NL
Cissel
D S
DS
Van Obberghen-Schilling
E
E
Baker
C C
CC
Kass
M E
ME
Ellingsworth
L R
LR
Roberts
A B
AB
Sporn
M B
MB
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002417:Cattle; D003110:Colonic Neoplasms / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D005109:Extracellular Matrix / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D007163:Immunosorbent Techniques; D051379:Mice; D008819:Mice, Nude; D010212:Papilloma / Q000032:analysis; D010446:Peptide Fragments / Q000276:immunology; D011498:Protein Precursors / Q000276:immunology; D011863:Radioimmunoassay; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology
D000906:Antibodies; D000939:Epitopes; D010446:Peptide Fragments; D011498:Protein Precursors; D014409:Tumor Necrosis Factor-alpha