title: Preliminary studies of the development of Anaplasma marginale in salivary glands of adult, feeding Dermacentor andersoni ticks.
abstract: On each day of feeding on susceptible calves, salivary glands obtained from groups of adult ticks that transmitted Anaplasma marginale were examined for A marginale colonies by use of light microscopy and transmission electron microscopy. On day 8 of feeding, salivary glands were examined, using fluorescein-labeled antibody and methyl green-pyronine stain. Use of fluorescein-labeled antibody consistently revealed small numbers of fluorescent foci in salivary gland acinar cells obtained from ticks that had fed for 8 days. Colonies of A marginale were seen by transmission electron microscopy only in salivary gland acini of male ticks; these colonies could not be identified, using light microscopy, in companion 1-micron plastic sections stained with Mallory stain. Methyl green-pyronine stain, used commonly to detect theilerial parasites in tick salivary glands, did not differentiate A marginale from cytoplasmic inclusions normally found in salivary gland acinar cells.
authors: Department of Veterinary Pathology, College of Veterinary Medicine, Oklahoma State University, Stillwater 74078.
authors: K M
authors: KM
authors: Kocan
authors: K B
authors: KB
authors: Wickwire
authors: S A
authors: SA
authors: Ewing
authors: J A
authors: JA
authors: Hair
authors: S J
authors: SJ
authors: Barron
mesh_terms: D000709:Anaplasma / Q000254:growth & development* / Q000648:ultrastructure; D000712:Anaplasmosis / Q000635:transmission; D000818:Animals; D001096:Arachnid Vectors / Q000382:microbiology*; D002417:Cattle; D003870:Dermacentor / Q000382:microbiology*; D005260:Female; D008297:Male; D008854:Microscopy, Electron; D012469:Salivary Glands / Q000382:microbiology; D013194:Staining and Labeling; D013987:Ticks / Q000382:microbiology*