title: Structural vs. contractile protein remodeling and myocardial stiffness in hypertrophied rat left ventricle.
abstract: Left ventricular pressure overload will result in the hypertrophic growth of the myocardium and in the rat may include a remodeling of both the structural and contractile proteins. As a result, an adaptive rise in active stiffness, or the force generating capacity of the myocardium, may occur. The relative importance of structural vs. contractile protein remodeling to the hypertrophic response and active stiffness is unclear. Accordingly, we monitored the ratio of V1/V3 isomyosin and the fibrillar nature and volume fraction of myocardial collagen, together with the developed systolic stress-strain relation of the intact myocardium in the adult male Wistar rat after 8 weeks of abdominal aorta banding. In comparison to controls and for the 20% increase in left ventricular mass obtained with banding we found: (a) collagen volume fraction had increased significantly (6.2 +/- 2.0 vs. 3.6 +/- 1.0%) while the V1/V3 ratio did not change; (b) interstitial compartment remodeling included a perivascular accumulation of collagen around small intramyocardial coronary arteries and the appearance of more extensive fibrillar collagens; and (c) active stiffness increased significantly. Thus, the increase in active stiffness of the hypertrophied adult rat myocardium, seen with abdominal aorta banding, appears to be related to interstitial fibrosis and not a conversion of myosin isoforms.
authors: Cardiovascular Institute, Michael Reese Hospital, University of Chicago Pritzker School of Medicine, IL 60616.
authors: J E
authors: JE
authors: Jalil
authors: C W
authors: CW
authors: Doering
authors: J S
authors: JS
authors: Janicki
authors: R
authors: R
authors: Pick
authors: W A
authors: WA
authors: Clark
authors: C
authors: C
authors: Abrahams
authors: K T
authors: KT
authors: Weber
mesh_terms: D000818:Animals; D001391:Azo Compounds; D006332:Cardiomegaly / Q000473:pathology / Q000503:physiopathology*; D003094:Collagen / Q000032:analysis*; D003238:Connective Tissue / Q000473:pathology; D006321:Heart / Q000503:physiopathology*; D006439:Hemodynamics; D008297:Male; D008859:Microscopy, Polarization; D009200:Myocardial Contraction; D009206:Myocardium / Q000032:analysis / Q000473:pathology*; D009218:Myosins / Q000032:analysis*; D010851:Picrates; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling
chemical_list: D001391:Azo Compounds; D010851:Picrates; C009798:C.I. direct red 80; D003094:Collagen; D009218:Myosins