[Interferon induction under in vitro conditions].
The procedures generally used for the in vitro induction of interferon exploit fibroblast or leucocyte culture suspensions. Several factors and conditions influencing the capacity for IFN induction were studied, with the following results. In contrast to previous findings, optimum IFN induction was obtained at 37 degrees C, rather than 30 degrees C, when human fibroblasts were induced with 100 micrograms/ml of Poly (IC). There was a direct correlation between the dose of the inducing agent applied and the amount of IFN produced. Also, the cells were excellently super-inducible with metabolic inhibitors like actinomycin D and cycloheximine. In addition, priming procedures enhanced IFN induction in human fibroblasts. Comparing different cell types with respect to their ability to be induced for IFN production, we found that different cell types show great differences in their inducer sensitivity. Finally, the response of different cell culture systems was highly variable when induced with different compounds.
Glück
B
B
Akademie der Wissenschaften der DDR, Zentral-institut für Mikrobiologie und experimentelle Therapie, Jena.
Stelzner
A
A
D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D005347:Fibroblasts / Q000187:drug effects / Q000276:immunology* / Q000382:microbiology; D006801:Humans; D007372:Interferons / Q000096:biosynthesis*; D007962:Leukocytes / Q000187:drug effects / Q000276:immunology / Q000382:microbiology; D010222:Parainfluenza Virus 1, Human / Q000254:growth & development; D011070:Poly I-C / Q000494:pharmacology; D013696:Temperature
D003609:Dactinomycin; D007372:Interferons; D003513:Cycloheximide; D011070:Poly I-C
Methodische Untersuchungen zur Interferon-Induktion unter in vitro-Bedingungen.