title: Characterization of content and chromatographic forms of neuropeptides in purified nerve varicosities prepared from guinea pig myenteric plexus.
abstract: Partially purified nerve varicosities (PV) prepared from guinea pig ileal myenteric plexus were found to contain, by radioimmunoassay, gastrin-releasing polypeptide (GRP), substance P (SP), galanin, Leu-enkephalin (LE), Met-enkephalin (ME), and vasoactive intestinal polypeptide (VIP). SP was present in the highest concentration followed by, in descending order, ME, LE, VIP, GRP and galanin. On reverse-phase HPLC, SP-, LE- and ME-like immunoreactivity in the PV preparation eluted at retention times similar to their synthetic analogues, galanin-like immunoreactivity eluted at a retention time different from that of synthetic porcine galanin and VIP-like immunoreactivity eluted at the retention time of synthetic guinea pig VIP. GRP-like immunoreactivity, on reverse-phase HPLC, eluted at retention times close to that of synthetic porcine GRP-(1-27) and its major oxidized form. Evidence was obtained for the presence of an alpha-neurokinin-like immunoreactive entity and an unidentified SP-like immunoreactive entity in guinea pig myenteric plexus.
authors: Department of Medicine, Robarts Research Institute University of Western Ontario, London, Canada.
authors: T J
authors: TJ
authors: McDonald
authors: F L
authors: FL
authors: Christofi
authors: B D
authors: BD
authors: Brooks
authors: W
authors: W
authors: Barnett
authors: M A
authors: MA
authors: Cook
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004743:Enkephalin, Leucine / Q000032:analysis; D004744:Enkephalin, Methionine / Q000032:analysis; D019004:Galanin; D019886:Gastrin-Releasing Peptide; D005768:Gastrointestinal Hormones / Q000032:analysis; D006168:Guinea Pigs; D007082:Ileum / Q000294:innervation; D009197:Myenteric Plexus / Q000032:analysis*; D009479:Neuropeptides / Q000032:analysis*; D010455:Peptides / Q000032:analysis; D013373:Substance P / Q000032:analysis; D014660:Vasoactive Intestinal Peptide / Q000032:analysis
chemical_list: D005768:Gastrointestinal Hormones; D009479:Neuropeptides; D010455:Peptides; D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D004744:Enkephalin, Methionine; D004743:Enkephalin, Leucine; D019886:Gastrin-Releasing Peptide; D019004:Galanin