In situ hybridization of nerve growth factor mRNA in the mouse submandibular gland.
We studied the presence of beta-nerve growth factor (NGF) mRNA in the submandibular gland of the mouse by in situ hybridization using 35S-labeled prepro-beta-NGF antisense RNA. Female and male mice were studied at different stages of postnatal development, ranging from 3 to 12 weeks. Although NGF mRNA was detectable in the granular convoluted tubules of the submandibular gland in all the age and sex groups studied, the abundance of the signal dramatically increased after 5 weeks during the development of the submandibular gland. In addition, a conspicuous sexual dimorphism became increasingly apparent in the 6-, 7-, 10-, and 12-week-old animals, due to the remarkable development of the granular convoluted tubules in the adult male mouse, that expressed abundant NGF mRNA.
Salido
E C
EC
Department of Pathology, Harbor-UCLA Medical Center, Torrance.
Yen
P H
PH
Shapiro
L J
LJ
Fisher
D A
DA
Barajas
L
L
D000818:Animals; D005260:Female; D008297:Male; D051379:Mice; D009414:Nerve Growth Factors / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D012313:RNA; D016372:RNA, Antisense; D012333:RNA, Messenger / Q000378:metabolism*; D012737:Sex Factors; D013363:Submandibular Gland / Q000502:physiology*
D009414:Nerve Growth Factors; D016372:RNA, Antisense; D012333:RNA, Messenger; D012313:RNA