[Structure of the repeating chain of the O-specific polysaccharide of Vibrio fluvialis].
O-Specific polysaccharide chain of the Vibrio fluvialis lipopolysaccharide is built up of pentasaccharide repeating units, containing one N-acetyl-D-glucosamine and four L-rhamnose residues. The structure of the polysaccharide was elucidated using two-dimensional correlation 1H-NMR-spectroscopy, 13C-NMR-spectroscopy and nuclear Overhauser effect and confirmed by methylation analysis and selective cleavage of N-acetylglucosamine residues by the N-deacetylation-deamination method which yielded linear L-rhamnan representing the backbone of the polysaccharide. Thus, the repeating unit of the O-specific polysaccharide has the following structure: (formula; see text)
Nazarenko
E L
EL
Gorshkova
R P
RP
Ovodov
Iu S
IuS
Shashkov
A S
AS
Knirel'
Iu A
IuA
D000107:Acetylation; D000942:Antigens, Bacterial; D002240:Carbohydrate Sequence; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D019081:O Antigens; D013379:Substrate Specificity; D014733:Vibrio / Q000276:immunology*
D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D019081:O Antigens
Struktura povtoriaiushchegosia zvena O-spetsificheskogo polisakharida Vibrio fluvialis.