The effects of 4-pentenoic and pentanoic acid on the hypoxic rat atria.
When exposed to hypoxia, the isolated rat atria released lactate into the bathing medium and underwent a rise in resting tension and a decline of the contractions frequency. In some of them, it also occurred a complete cessation of the pacemaker activity. Atria from 24-h fasted rats, when compared to those from fed ones, exhibited a lower lactate output, a higher rise in resting tension, a faster decay of the contraction frequency and an increased occurrence of atrial arrest. In both the fed and fasted rats atria, some triacylglycerol lipolysis remained throughout the hypoxic incubation. Addition of 2 mM 4-pentenoic acid abolished the lipolytic activity and reduced lactate output in both groups of atria. In the fed rats atria it also accelerated the decrease of the pacemaker frequency. Pentanoic acid reduced lactate output in both groups of atria and in those from fed rats it did not alter lipolysis but increased the rise in resting tension, the decline of the pacemaker frequency and the occurrence of atrial arrest. Present data indicate that although 4-pentenoic acid inhibits fatty acid oxidation and endogenous lipolysis, it was not able to reduce the noxious effects of hypoxia. Since the effects of 4-pentenoic acid were rather similar to those of fasting and pentanoic acid, they might be ascribed to the accumulation of its own oxidative metabolites which could be detrimental for the hypoxic atria.
Varela
A
A
Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
Lanzetta
D
D
Savino
E A
EA
D000818:Animals; D005215:Fasting / Q000502:physiology; D005229:Fatty Acids, Monounsaturated / Q000494:pharmacology*; D005260:Female; D006325:Heart Atria / Q000187:drug effects / Q000503:physiopathology; D000860:Hypoxia / Q000503:physiopathology*; D066298:In Vitro Techniques; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008066:Lipolysis / Q000187:drug effects; D009200:Myocardial Contraction / Q000187:drug effects*; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D014280:Triglycerides / Q000378:metabolism; D014631:Valerates / Q000494:pharmacology*
D005229:Fatty Acids, Monounsaturated; D007773:Lactates; D014280:Triglycerides; D014631:Valerates; D019344:Lactic Acid; C009785:4-pentenoic acid