Prothymosin alpha is an evolutionary conserved protein covalently linked to a small RNA.
A 13 kDa protein, covalently linked to a small RNA from the cytoplasm of mouse cells, was studied. Sequence analysis of its tryptic peptides revealed that the RNA-linked protein is identical to prothymosin alpha. Very similar RNA-protein complexes were identified in human, bovine and yeast cells. Tryptic peptide maps of 125I-labelled RNA-linked proteins of diverse origin demonstrated their marked similarity, thus indicating high evolutionary conservation of prothymosin alpha from yeast to man.
Makarova
T
T
Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.
Grebenshikov
N
N
Egorov
C
C
Vartapetian
A
A
Bogdanov
A
A
D000595:Amino Acid Sequence; D000818:Animals; D005075:Biological Evolution; D002460:Cell Line; D003593:Cytoplasm / Q000378:metabolism; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments; D010449:Peptide Mapping; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*; D012441:Saccharomyces cerevisiae; D013947:Thymosin / Q000378:metabolism / Q000031:analogs & derivatives
D010446:Peptide Fragments; D011498:Protein Precursors; C040782:prothymosin alpha; D013947:Thymosin; D012313:RNA