Subcellular localization of cyclo-oxygenase-prostaglandin E2 synthetase complex in goat vesicular gland by catalytic activity analysis.
The catalytic activity of the cyclo-oxygenase prostaglandin E2 synthetase complex in subcellular organelles of goat vesicular gland was determined. The enzyme activity was found to be located mostly in the rough endoplasmic reticulum and partly in the nuclear membrane; comparatively very little activity could be detected in the smooth endoplasmic reticulum. There was no detectable activity of the enzyme in the plasma membrane.
Mukherjee
E
E
Department of Chemistry, Bose Institute, Calcutta, India.
Ghosh
D
D
D000818:Animals; D002384:Catalysis; D002462:Cell Membrane / Q000201:enzymology; D002499:Centrifugation, Density Gradient; D015232:Dinoprostone / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000201:enzymology; D005088:Exocrine Glands / Q000378:metabolism*; D006041:Goats; D066298:In Vitro Techniques; D008297:Male; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D012669:Seminal Vesicles / Q000201:enzymology / Q000648:ultrastructure; D013347:Subcellular Fractions / Q000201:enzymology*
D011506:Proteins; D012313:RNA; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone