Heat stress in the presence of low RNA polymerase activity increases chromosome copy number of Escherichia coli.
A temperature shift-up accompanied by a reduction in RNA polymerase activity in Escherichia coli causes an increased rate of initiation leading to a 1.7- to 2.2-fold increase in chromosome copy number. A temperature shift-up without a reduction in polymerase activity induces only a transient non-scheduled initiation of chromosome replication caused by heat shock with no detectable effect on chromosome copy number.
Guzman
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EC
Departamento de Bioquimica y Biologia Molecular y Genetica, Universidad de Extremadura, Badajoz, Spain.
Jimenez-Sanchez
A
A
Orr
E
E
Pritchard
R H
RH
D002876:Chromosomes, Bacterial / Q000502:physiology*; D004269:DNA, Bacterial / Q000096:biosynthesis; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D006358:Hot Temperature; D007700:Kinetics; D010957:Plasmids; D012329:RNA, Bacterial / Q000096:biosynthesis; D013997:Time Factors
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012321:DNA-Directed RNA Polymerases