[Changes in monoamine metabolites in the nucleus accumbens and locomotor activity by reserpine: an in vivo microdialysis study].
The effect of ip administration of reserpine (2 mg/kg) on the monoamine metabolites 3,4-dihydroxyphenyl acid (DOPAC), homovanillic acid (HVA), 5-hydroxy-indoleacetic acid (5-HIAA) was estimated in the freely moving rat by intracerebral dialysis in the nucleus accumbens. Locomotor activity was measured simultaneously by Automex. Locomotor activity tended to increase during the first 2 hours. On the other hand, a significant reduction of locomotor activity was found during the 3rd-5th hour period of the experiment. HVA-total (conjugated + free) and 5-HIAA were elevated significantly (P less than 0.05) during the 3rd-5th and the 5th-6th hour periods, respectively. However, the difference of DOPAC between both groups did not reach statistical significance throughout the experiment. During the 3rd-5th hour of experiment, the change of behavior and HVA were inversely correlated. This might suggest that an HVA level in the dialysate is a good index of dopamine metabolism in the nucleus accumbens after reserpine treatment.
Sugita
R
R
Department of Psychiatry, Fujita-Gakuen Health University School of Medicine, Toyoake, Japan.
Sawa
Y
Y
Nakazawa
T
T
Yamauchi
T
T
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism*; D000818:Animals; D003956:Dialysis; D006719:Homovanillic Acid / Q000378:metabolism*; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism*; D008297:Male; D009043:Motor Activity / Q000187:drug effects*; D009714:Nucleus Accumbens / Q000378:metabolism*; D010648:Phenylacetates / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D012110:Reserpine / Q000494:pharmacology*; D012686:Septal Nuclei / Q000378:metabolism*
D010648:Phenylacetates; D015102:3,4-Dihydroxyphenylacetic Acid; D006897:Hydroxyindoleacetic Acid; D012110:Reserpine; D006719:Homovanillic Acid