A slow kinetic transient in RNA synthesis catalysed by wheat-germ RNA polymerase II.
Progress curves of U-A-primed RNA synthesis catalysed by wheat-germ RNA polymerase II on a poly[d(A-T)] template exhibit a slow burst of activity. In contrast, the progress curves of single-step addition of UMP to U-A primer in the abortive elongation reaction do not exhibit the slow burst of activity. The correlation between the kinetic transient in the productive pathway of RNA synthesis and the rate of abortive elongation is suggestive of the occurrence of a slow conformational change of the transcription complex during the transition from abortive to productive elongation. The exceptional duration of the transient burst (in the region of 4 min) may suggest a transition of a hysteretic type.
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Centre de Biochimie et de Biologie Moléculaire, Centre National de la Recherche Scientifique, Marseille, France.
De Mercoyrol
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D000645:Ammonium Sulfate / Q000494:pharmacology; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D011067:Poly dA-dT / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis*; D012319:RNA Polymerase II / Q000378:metabolism*; D014908:Triticum / Q000201:enzymology*; D014542:Uridine Monophosphate / Q000378:metabolism
D011067:Poly dA-dT; D012313:RNA; D014542:Uridine Monophosphate; D012319:RNA Polymerase II; D008274:Magnesium; D000645:Ammonium Sulfate