Cyclic AMP: a second messenger of the newly characterized AVP-like insect diuretic hormone, the migratory locust diuretic hormone.
An AVP-like neurohormone was extracted from suboesophageal and thoracic ganglia of Locusta migtatoria, isolated, characterized and synthesized. It functions as a diuretic hormone in this species by enhancing the excretion of urine from the MT. It appears to act by increasing cyclic AMP; synthetic AVP-like IDH increases cyclic AMP of the MT in vitro in a time-dependant, dose-dependant and very specific manner. 8-BR-cyclic AMP, an analog of cyclic AMP known to enter the cells, mimics the diuretic action of the AVP-like IDH. Furthermore, the combined actions of forskolin (the activator of the adenylate cyclase) and IBMX (the inhibitor of the phosphodiestherase activity) increase both MT cyclic AMP level and excretion of the primary urine. We conclude from these results that cyclic AMP is a second messenger of the AVO-like IDH.
Proux
J P
JP
Laboratoire de Neuroendocrinologie, UA CNRS 1138, Université de Bordeaux I, Talence, France.
Herault
J P
JP
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001127:Arginine Vasopressin / Q000502:physiology*; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D006110:Grasshoppers / Q000502:physiology*; D007301:Insect Hormones / Q000502:physiology*; D008317:Malpighian Tubules / Q000187:drug effects; D008969:Molecular Sequence Data; D011863:Radioimmunoassay
D000596:Amino Acids; D007301:Insect Hormones; D001127:Arginine Vasopressin; D005576:Colforsin; D000242:Cyclic AMP; D015056:1-Methyl-3-isobutylxanthine