[Comparison of methods for the identification of Campylobacter pylori in gastric biopsies of patients with dyspepsia].
Campylobacter pylori has been associated with gastro-duodenal inflammatory disease. Ninety-five adults with dyspepsia were examined for the presence of C. pylori in the gastric antrum and near gastric or duodenal ulcers (when present) by means of culture, Gram and acridine orange stains, and urease activity of biopsies. C. pylori was identified from 51 out of 67 patients with chronic gastritis, from 9 out of 9 patients with duodenal ulcer, and from 8 out of 10 patients with gastric ulcer. Acridine orange stain revealed the highest number of positive cases, followed by culture, Gram stain and urease test. The latter showed a 100% specificity when carried out with a selective urea broth containing colistin, trimethoprim, vancomycin and amphotericin B. It has to be considered a further diagnostic tool which enables clinicians and microbiologists to diagnose the etiology of a dyspeptic syndrome even at the patient's bedside.
Guglielmetti
P
P
Istituto di Clinica delle Malattie Infettive Università, Siena.
Quaranta
S
S
Figura
N
N
Fanteria
E
E
Rossolini
A
A
Camarri
E
E
D000165:Acridine Orange; D000328:Adult; D001426:Bacterial Proteins / Q000032:analysis; D001431:Bacteriological Techniques; D001706:Biopsy; D002167:Campylobacter / Q000302:isolation & purification*; D003470:Culture Media; D004381:Duodenal Ulcer / Q000150:complications / Q000382:microbiology; D004415:Dyspepsia / Q000209:etiology / Q000382:microbiology*; D005840:Gentian Violet; D006801:Humans; D010619:Phenazines; D011237:Predictive Value of Tests; D013194:Staining and Labeling; D013270:Stomach / Q000382:microbiology*; D013276:Stomach Ulcer / Q000150:complications / Q000382:microbiology; D014510:Urease / Q000032:analysis
D001426:Bacterial Proteins; D003470:Culture Media; C035863:Gram's stain; D010619:Phenazines; D014510:Urease; D000165:Acridine Orange; D005840:Gentian Violet
Confronto di metodiche per l'identificazione di "Campylobacter pylori" in biopsie gastriche di pazienti con dispepsia.