High affinity human IFN-gamma-binding capacity is encoded by a single receptor gene located in proximity to c-ros on human chromosome region 6q16 to 6q22.
We have used human-rodent somatic cell hybrids to investigate the regional localization of the IFN-gamma R gene on human chromosome 6 and studied functional and antigenic characteristics of the expressed IFN-gamma R by Scatchard analyses of 125I-IFN-gamma binding and binding of an anti-receptor mAb (A6C5). The data obtained revealed coordinate expression of IFN-gamma- and A6C5-binding capacity as well as competition in binding to chromosome 6-positive hybrids and normal cells, indicating that the A6C5-defined protein is by itself capable of high affinity IFN-gamma binding and, thus, is likely to constitute the major IFN-gamma R protein of distinct cell types. The receptor gene could be allocated to region 6q16 to 6q22, which also contains the c-ros oncogene. Genetic linkage of the IFN-gamma R gene to an oncogene located in a region of non-random chromosomal aberrations may have a causal relationship to the deregulated IFN-gamma R expression in several malignancies.
Pfizenmaier
K
K
Clinical Research Group, Max-Planck-Society, Göttingen, Federal Republic of Germany.
Wiegmann
K
K
Scheurich
P
P
Krönke
M
M
Merlin
G
G
Aguet
M
M
Knowles
B B
BB
Ucer
U
U
D000911:Antibodies, Monoclonal; D001666:Binding Sites, Antibody; D002460:Cell Line; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6; D000939:Epitopes / Q000032:analysis; D008040:Genetic Linkage; D006801:Humans; D006822:Hybrid Cells / Q000032:analysis; D007371:Interferon-gamma / Q000378:metabolism*; D009857:Oncogenes; D011971:Receptors, Immunologic / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D017471:Receptors, Interferon; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000939:Epitopes; D011971:Receptors, Immunologic; D017471:Receptors, Interferon; D007371:Interferon-gamma