Antioxidants for bone marrow cryopreservation.
Addition of antioxidants into the preservation solution improved the cryoprotection of human bone marrow cells. The viability was studied by the growth of GM-CFC in agar culture before and after storage at -196 degrees C. All used antioxidative drugs (selenomethionine, methionine, tocopherol, penicillin/Fe++) increased the tolerance of the stem cells to freezing and thawing and elevated the number of surviving GM-CFC up to the twofold in comparison with that of controls. More immature colony forming cells were especially protected.
Matthes
G
G
Institute of Transfusiology and Transplantology, Medical School (Charité), Humboldt University, Berlin.
Hübner
U
U
Gränz
M
M
Schütt
M
M
D000975:Antioxidants; D001853:Bone Marrow; D001854:Bone Marrow Cells; D002455:Cell Division; D015925:Cryopreservation; D005296:Ferrous Compounds; D006098:Granulocytes / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D008264:Macrophages / Q000166:cytology; D008715:Methionine; D010406:Penicillins; D012645:Selenomethionine; D014021:Tissue Preservation / Q000379:methods*; D014810:Vitamin E
D000975:Antioxidants; D005296:Ferrous Compounds; D010406:Penicillins; D014810:Vitamin E; D012645:Selenomethionine; D008715:Methionine