A conditional high-copy-number plasmid derivative of pBR322.
A spontaneous high-copy-number plasmid derivative of plasmid pBR322 was isolated. This plasmid bears two point mutations adjacent to the unpaired region in the stem of loop II of RNA I and expresses its high-copy-number phenotype only when the harboring cells grow on solid support (L-agar).
Ivanov
I
I
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
Yavashev
L
L
Gigova
L
L
Alexciev
K
K
Christov
C
C
D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis*; D009154:Mutation; D009690:Nucleic Acid Conformation; D010641:Phenotype; D010957:Plasmids; D012329:RNA, Bacterial; D012093:Replicon
D004269:DNA, Bacterial; D012329:RNA, Bacterial