Inhibition of HIV replication in cell culture by the specific aspartic protease inhibitor pepstatin A.
After incubation of H9 cells infected with human immunodeficiency virus (HIV) with pepstatin A at 10(-4) M for 2, 4, or 11 days, the culture medium contained significantly less HIV core antigen (p24) than controls without pepstatin A and no or only borderline activity of reverse transcriptase was detected. In addition, after pepstatin A treatment no infectious HIV at 2 or 4 days and only minimal amounts at 11 days were detectable in the culture medium.
von der Helm
K
K
Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig-Maximilians University of Munich, FRG.
Gürtler
L
L
Eberle
J
J
Deinhardt
F
F
D016282:Aspartic Acid Endopeptidases; D002460:Cell Line; D010450:Endopeptidases; D006678:HIV / Q000276:immunology / Q000502:physiology*; D015488:HIV Antigens / Q000096:biosynthesis; D016655:HIV Core Protein p24; D009842:Oligopeptides / Q000494:pharmacology*; D010436:Pepstatins / Q000494:pharmacology*; D011480:Protease Inhibitors; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012191:Retroviridae Proteins / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects*
D015488:HIV Antigens; D016655:HIV Core Protein p24; D009842:Oligopeptides; D010436:Pepstatins; D011480:Protease Inhibitors; D012191:Retroviridae Proteins; C014195:Streptomyces pepsin inhibitor; D012194:RNA-Directed DNA Polymerase; D010450:Endopeptidases; D016282:Aspartic Acid Endopeptidases; C031375:pepstatin