[Effect of triiodothyronine on nucleic acid synthesis of immunocytes in cell culture].
A direct action of T3 on the synthesis of nucleic acids in immunocytes in a cell culture was studied. Scintillation radiometry has shown that T3 makes a regulatory effect on anabolism of nucleic acids in thymocytes and peripheral blood mononuclears at certain stimulating doses of the hormone. T- and B-splenocytes were characterized by metabolic inertness with relation to T3. Serum absence in a culture medium did not prevent the activation of RNA and DNA synthesis in thymocytes. Optimum mitogenic doses of the hormone in a serum-free medium were lower than those in a conditioned culture medium. A study of the kinetics of RNA and DNA synthesis activation by T3 has shown that a regulatory action of the hormone results in the activation of RNA synthesis "triggering". This conclusion is confirmed by evidence on inhibition of G0-G1 transition of the cellular cycle by antiserum to T3. T3 influence on DNA synthesis activation is mediated by a rise of the level of cellular RNA. It produces no direct effect on the G0-G1 phase of the cellular cycle.
Baeva
E V
EV
D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000378:metabolism / Q000502:physiology; D008297:Male; D012313:RNA / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D012588:Scintillation Counting; D013154:Spleen / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D014284:Triiodothyronine / Q000502:physiology*
D014284:Triiodothyronine; D012313:RNA; D004247:DNA
Vliianie triĭodtironina na sintez nukleinovykh kislot immunotsitami v kul'ture kletok.