Specific block of calcium channel expression by a fragment of dihydropyridine receptor cDNA.
Although the structure of rabbit skeletal muscle dihydropyridine (DHP) receptor, deduced from cDNA sequence, indicates that this protein is the channel-forming subunit of voltage-dependent calcium channel (VDCC), no functional proof for this prediction has been presented. Two DNA oligonucleotides complementary to DHP-receptor RNA sequences coding for putative membrane-spanning regions of the DHP receptor specifically suppress the expression of the DHP-sensitive VDCC from rabbit and rat heart in Xenopus oocytes. However, these oligonucleotides do not suppress the expression of the DHP-insensitive VDCC and of voltage-dependent sodium and potassium channels. Thus, the gene for DHP receptor of rabbit skeletal muscle is closely related, or identical to, a gene expressed in heart that encodes a component of the DHP-sensitive VDCC. The DHP-sensitive and DHP-insensitive VDCCs are distinct molecular entities.
Lotan
I
I
Division of Physiology and Pharmacology, Sackler School of Medicine, Tel Aviv University, Ramat Aviv, Israel.
Goelet
P
P
Gigi
A
A
Dascal
N
N
D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / Q000494:pharmacology; D000818:Animals; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology*; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D004553:Electric Conductivity; D005786:Gene Expression Regulation; D009132:Muscles / Q000032:analysis; D009206:Myocardium / Q000032:analysis; D009693:Nucleic Acid Hybridization; D009865:Oocytes / Q000502:physiology; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D051381:Rats; D011978:Receptors, Nicotinic / Q000235:genetics*; D014981:Xenopus
D015220:Calcium Channels; D015342:DNA Probes; D012333:RNA, Messenger; D011978:Receptors, Nicotinic; D012313:RNA; D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; D004247:DNA