Mitochondrial porin regulates the sensitivity of anion carriers to inhibitors.
In mitoplasts, respiratory stimulation by ADP, palmitate, DNP and CCCP and sensitivity of respiration to carboxyatractylate are considerably less pronounced than in mitochondria. Addition of porin-containing preparations (purified outer membranes or solubilized mitochondrial porin) to mitoplasts results in partial restoration of the oxygen consumption and sensitivity to carboxyatractylate (CAT). The uncoupling effect of FCCP in mitoplasts is CAT-resistant and does not depend on added porin. It is suggested that mitochondrial porin may be a natural activator of ADP/ATP antiporter and succinate carrier in mitochondria.
Pronevich
L A
LA
Institute of Biological Physics, USSR Academy of Sciences, Pushchino, Moscow Region.
Mirzabekov
T A
TA
Rozhdestvenskaya
Z E
ZE
D019297:2,4-Dinitrophenol; D000244:Adenosine Diphosphate / Q000378:metabolism / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D027321:Anion Transport Proteins; D001278:Atractyloside / Q000031:analogs & derivatives / Q000494:pharmacology; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002352:Carrier Proteins / Q000037:antagonists & inhibitors*; D003574:Cytochrome c Group / Q000494:pharmacology; D004140:Dinitrophenols / Q000494:pharmacology; D007425:Intracellular Membranes / Q000187:drug effects / Q000378:metabolism; D007473:Ion Channels; D008565:Membrane Proteins / Q000494:pharmacology*; D008930:Mitochondria, Liver / Q000032:analysis / Q000187:drug effects / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects*; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000494:pharmacology; D018272:Porins; D051381:Rats; D013386:Succinates / Q000378:metabolism; D019802:Succinic Acid; D050994:Voltage-Dependent Anion Channels
D027321:Anion Transport Proteins; D002352:Carrier Proteins; D003574:Cytochrome c Group; D004140:Dinitrophenols; D007473:Ion Channels; D008565:Membrane Proteins; D010169:Palmitic Acids; D018272:Porins; D013386:Succinates; D050994:Voltage-Dependent Anion Channels; D001278:Atractyloside; D019308:Palmitic Acid; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D019802:Succinic Acid; D019297:2,4-Dinitrophenol; C003853:carboxyatractyloside