title: [Degradation of messenger RNA, ribosomal RNA and 2-5A-dependent inhibition of protein biosynthesis].
abstract: In rabbit reticulocyte lysates the addition of exogenous 2-5A leads after 10-20 minutes to the inhibition of protein synthesis. This inhibition can be blocked by rat antiserum to 2-5A. In intact ribosomes the ribosomal RNA is cleaved after 2-5A addition, but this cleavage is not in correlation with the protein synthesis shutoff. Ribosomal 5S RNA and 5,8S RNA are not cleaved even after several hours of incubation with 2-5A. The degradation of polysome associated mRNA correlates with the protein synthesis inhibition as revealed by Northern blot hybridization of globin mRNA with 32P-labelled p beta G plasmid. The addition of 2-5A antiserum to the rabbit reticulocyte lysate also inhibits the degradation of polysome bound globin mRNA.
authors: U E
authors: UE
authors: Toots
authors: M B
authors: MB
authors: Kel've
authors: M Iu
authors: MIu
authors: Saarma
mesh_terms: D015088:2',5'-Oligoadenylate Synthetase / Q000378:metabolism; D000227:Adenine Nucleotides / Q000378:metabolism*; D000818:Animals; D001609:Beta-Globulins / Q000378:metabolism; D015152:Blotting, Northern; D004247:DNA; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D007106:Immune Sera; D009693:Nucleic Acid Hybridization; D009843:Oligoribonucleotides / Q000378:metabolism*; D014176:Protein Biosynthesis; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D012260:Ribonucleases / Q000378:metabolism
chemical_list: D000227:Adenine Nucleotides; D001609:Beta-Globulins; D007106:Immune Sera; D009843:Oligoribonucleotides; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D012335:RNA, Ribosomal; C023505:2',5'-oligoadenylate; D004247:DNA; D015088:2',5'-Oligoadenylate Synthetase; D012260:Ribonucleases
vernacular_title: Degradatsiia matrichnoĭ RNK, ribosomnoĭ RNK i 2-5A-zavisimoe ingibirovanie biosinteza belka.