The developmental regulation of the L2/HNK-1 and L3 carbohydrate epitopes in mouse brain. Evidence for separate control of lipid- and protein-bound epitopes.
The carbohydrate epitopes L2/HNK-1 and L3 have previously been identified on various neural cell adhesion molecules and have been suggested to play a role in the mediation of cell-cell adhesion. In this study, the developmental expression of the two epitopes in soluble, membrane-bound and chloroform/methanol-extracted fractions of the constituent mouse brain regions was examined by enzyme-linked immunosorbent assay (ELISA). The protein-bound epitopes were shown to be uniformly developmentally regulated, with levels peaking at postnatal day 20 (P20). The epitopes in a crude chloroform/methanol fraction, however, demonstrated a different pattern, with L2 peaking earlier at postnatal day zero (P0). These results suggest a possible interaction between the control of the two pools of the epitope.
Breen
K C
KC
Dept of Immunology, St. Georges Hospital Medical School, London, England.
D000375:Aging; D000818:Animals; D000943:Antigens, Differentiation / Q000032:analysis*; D000954:Antigens, Surface / Q000276:immunology; D001921:Brain / Q000254:growth & development* / Q000276:immunology; D001933:Brain Stem / Q000276:immunology; D018959:CD57 Antigens; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis* / Q000276:immunology; D007031:Hypothalamus / Q000276:immunology; D008055:Lipids / Q000276:immunology*; D008562:Membrane Glycoproteins; D008636:Mesencephalon / Q000276:immunology; D051379:Mice; D009419:Nerve Tissue Proteins / Q000276:immunology*; D013116:Spinal Cord / Q000276:immunology; D014018:Tissue Distribution
D000943:Antigens, Differentiation; D000954:Antigens, Surface; D018959:CD57 Antigens; D015815:Cell Adhesion Molecules; D000939:Epitopes; D008055:Lipids; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins