Characterization of content and chromatographic forms of neuropeptides in purified nerve varicosities prepared from guinea pig myenteric plexus.
Partially purified nerve varicosities (PV) prepared from guinea pig ileal myenteric plexus were found to contain, by radioimmunoassay, gastrin-releasing polypeptide (GRP), substance P (SP), galanin, Leu-enkephalin (LE), Met-enkephalin (ME), and vasoactive intestinal polypeptide (VIP). SP was present in the highest concentration followed by, in descending order, ME, LE, VIP, GRP and galanin. On reverse-phase HPLC, SP-, LE- and ME-like immunoreactivity in the PV preparation eluted at retention times similar to their synthetic analogues, galanin-like immunoreactivity eluted at a retention time different from that of synthetic porcine galanin and VIP-like immunoreactivity eluted at the retention time of synthetic guinea pig VIP. GRP-like immunoreactivity, on reverse-phase HPLC, eluted at retention times close to that of synthetic porcine GRP-(1-27) and its major oxidized form. Evidence was obtained for the presence of an alpha-neurokinin-like immunoreactive entity and an unidentified SP-like immunoreactive entity in guinea pig myenteric plexus.
McDonald
T J
TJ
Department of Medicine, Robarts Research Institute University of Western Ontario, London, Canada.
Christofi
F L
FL
Brooks
B D
BD
Barnett
W
W
Cook
M A
MA
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004743:Enkephalin, Leucine / Q000032:analysis; D004744:Enkephalin, Methionine / Q000032:analysis; D019004:Galanin; D019886:Gastrin-Releasing Peptide; D005768:Gastrointestinal Hormones / Q000032:analysis; D006168:Guinea Pigs; D007082:Ileum / Q000294:innervation; D009197:Myenteric Plexus / Q000032:analysis*; D009479:Neuropeptides / Q000032:analysis*; D010455:Peptides / Q000032:analysis; D013373:Substance P / Q000032:analysis; D014660:Vasoactive Intestinal Peptide / Q000032:analysis
D005768:Gastrointestinal Hormones; D009479:Neuropeptides; D010455:Peptides; D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D004744:Enkephalin, Methionine; D004743:Enkephalin, Leucine; D019886:Gastrin-Releasing Peptide; D019004:Galanin