Effect of insulin on the growth of a human hepatoma cell line PLC/PRF/5: a possible role of insulin receptor.
Insulin produced increases in cell number, DNA synthesis and alpha-fetoprotein secretion of a human hepatoma cell line PLC/PRF/5. The insulin-treated cells showed a decrease in binding of 125I-insulin. The decreased binding was due to the decreased numbers as well as the low affinity constant of insulin receptor. These data suggest that insulin is a growth factor for the human hepatoma cells and that insulin receptor plays a role in the growth-promoting effect of insulin.
Motoo
Y
Y
First Department of Internal Medicine, School of Medicine, Kanazawa University, Kanazawa 921, Japan.
Kobayashi
K
K
Hattori
N
N
D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D008113:Liver Neoplasms; D011972:Receptor, Insulin / Q000502:physiology*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014407:Tumor Cells, Cultured; D000509:alpha-Fetoproteins / Q000378:metabolism
D004273:DNA, Neoplasm; D007328:Insulin; D000509:alpha-Fetoproteins; D014316:Tritium; D011972:Receptor, Insulin; D013936:Thymidine