An in vitro system for screening anti-hepatitis B virus drugs.
A human hepatoblastoma cell line (HB 611) that continuously synthesizes hepatitis B viral (HBV) DNA was grown in the presence of various inhibitors of DNA synthesis, and the DNA from the cells was analyzed by the Southern blotting method to examine selective inhibition of the viral DNA synthesis. Among those that showed selective inhibition, and interferons alpha and beta, acyclovir, and dideoxy cytidine were effective. This system should be useful for screening new antiviral agents against HBV.
Ueda
K
K
Institute for Molecular and Cellular Biology, Osaka University, Suita, Japan.
Tsurimoto
T
T
Nagahata
T
T
Chisaka
O
O
Matsubara
K
K
D000212:Acyclovir / Q000494:pharmacology; D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology*; D015139:Blotting, Southern; D006528:Carcinoma, Hepatocellular; D003561:Cytarabine / Q000494:pharmacology; D004261:DNA Replication / Q000187:drug effects; D004279:DNA, Viral / Q000032:analysis / Q000096:biosynthesis; D016048:Dideoxyadenosine; D015224:Dideoxynucleosides / Q000494:pharmacology; D006515:Hepatitis B virus / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D007372:Interferons / Q000494:pharmacology; D008113:Liver Neoplasms; D014407:Tumor Cells, Cultured; D014740:Vidarabine / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects; D016047:Zalcitabine; D015215:Zidovudine / Q000494:pharmacology
D000998:Antiviral Agents; D004279:DNA, Viral; D015224:Dideoxynucleosides; D003561:Cytarabine; C051061:oxetanocin; D015215:Zidovudine; D016048:Dideoxyadenosine; D016047:Zalcitabine; D007372:Interferons; D014740:Vidarabine; D000225:Adenine; D000212:Acyclovir