Expression of c-myb in embryonal carcinoma cells and embryonal stem cells.
Mouse c-myb has been implicated in the regulation of differentiation and proliferation of haematopoietic cells. Analysis of the chromatin structure of the promoter region of c-myb in embryonal carcinoma (EC) cells and embryonal stem (ES) cells reveals a DNAse I-hypersensitive site coincident with a site found in c-myb-expressing haematopoietic cells, but absent in murine fibroblasts (which do not express c-myb). EC and ES cells were found to express c-myb mRNA, albeit at a level lower than found in haematopoietic cells. Differentiation of ES cells into embryoid bodies resulted in an elevated level of c-myb expression.
Dyson
P J
PJ
Department of Genetics, University of Cambridge, UK.
Poirier
F
F
Watson
R J
RJ
D000818:Animals; D003850:Deoxyribonuclease I / Q000032:analysis; D054278:Embryonal Carcinoma Stem Cells; D015870:Gene Expression; D006410:Hematopoiesis / Q000235:genetics; D051379:Mice; D009373:Neoplasms, Germ Cell and Embryonal / Q000032:analysis / Q000378:metabolism* / Q000473:pathology; D014411:Neoplastic Stem Cells / Q000032:analysis / Q000378:metabolism* / Q000473:pathology; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D020598:Proto-Oncogene Proteins c-myb; D012313:RNA / Q000032:analysis; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured / Q000032:analysis / Q000187:drug effects / Q000378:metabolism
D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb; D012313:RNA; D003850:Deoxyribonuclease I