[Cloning and determination of the primary structure of cDNA, coding the central part of elongation factor 2 from the rat liver].
A number of cDNA clones coding for 417 amino acid residues of the central part of the rat liver elongation factor 2 (EF-2) have been isolated. The oligonucleotides complementary to EF-2 mRNA were used as primers for synthesis of the first strand of cDNA cloned. Structures of these oligonucleotides were determined in course of 3'----5' sequencing coding strand of EF-2 cDNA. This method of synthesis of specific cDNA enabled one to reduce essentially the number of recombinant clones to be screened for EF-2 cDNA. Comparative studies of deduced protein sequences of rat liver EF-2 and hamster EF-2 [1] revealed the only substitution of aspartate residue for glutamate residue (hamster EF-2). The homology between nucleotide sequences of rat and hamster EF-2 cDNA was 89%. Northern-blot analysis of rat liver poly(A)+ mRNA revealed the only species of mRNA 3000 nucleotides long. A strong stop-signal for reverse transcriptase in the 5'-region of rat liver EF-2 mRNA is discovered: probability of dissociation of the enzyme from mRNA is at least 97.5%.
Oleĭnikov
A V
AV
Dzhokhadze
G G
GG
Alakhov
Iu B
IuB
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA / Q000235:genetics*; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012194:RNA-Directed DNA Polymerase / Q000235:genetics; D051381:Rats; D012689:Sequence Homology, Nucleic Acid
D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors; D012333:RNA, Messenger; D004247:DNA; D012194:RNA-Directed DNA Polymerase
Klonirovanie i opredelenie pervichnoĭ struktury kDNK, kodiruiushcheĭ tsentral'nuiu chast' faktora élongatsii 2 iz pecheni krysy.