title: High-level expression and epitope localization of the major outer membrane protein of Chlamydia trachomatis serovar L1.
abstract: Fragments of the gene encoding the major outer membrane porin protein (MOMP) of Chlamydia trachomatis serovar L1 were ligated into the pUC plasmid vectors to give a series of overlapping recombinants expressing MOMP from the lac promoter. Induction of this promoter with IPTG leads to high-level expression of the recombinant porin protein. Electron microscopy shows the presence of insoluble inclusions within the Escherichia coli host cells. Probing the expressed MOMP fragments with a set of monoclonal antibodies permitted localization of the four binding sites (epitopes) of primary-sequence-dependent monoclonal antibodies that exhibit genus-, species-, subspecies- and type (serovar)-specific reactivities.
authors: Department of Microbiology, University of Southampton Medical School, Southampton General Hospital, UK.
authors: M A
authors: MA
authors: Pickett
authors: M E
authors: ME
authors: Ward
authors: I N
authors: IN
authors: Clarke
mesh_terms: D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis* / Q000276:immunology / Q000648:ultrastructure; D015153:Blotting, Western; D002692:Chlamydia trachomatis / Q000276:immunology / Q000378:metabolism*; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D005822:Genetic Vectors; D008854:Microscopy, Electron; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000276:immunology / Q000648:ultrastructure; D015183:Restriction Mapping; D012703:Serotyping
chemical_list: D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D000939:Epitopes; D011993:Recombinant Fusion Proteins