Contributions of negatively charged chemical groups to the surface-dependent activation of human plasma by soluble dextran derivatives.
Negatively charged surfaces are known to promote contact activation. The mechanism responsible for increasing affinity for surfaces is not yet quite understood, although the presence of negative charge densities is thought to be a prerequisite. With the availability of soluble dextran derivatives, varyingly substituted with charged methylcarboxylate, methylbenzylamide sulphonate and uncharged methylbenzylamide residues, we were able to discriminate between the contributions of these chemical moieties to contact activation, thus suggesting that the stimulating properties of synthetic negatively charged surfaces should also be described in terms of specific interactions instead of global negative charge density. This could be effected by quantifying the activating capacities as a function of the chemical group composition. A direct correlation linking activating capacities to anticoagulant properties has been observed.
Nigretto
J M
JM
Laboratoire de Chimie Bioanalytique, Université de Tours, France.
Corretge
E
E
Jozefowicz
M
M
D000327:Adsorption; D001672:Biocompatible Materials; D001777:Blood Coagulation / Q000502:physiology*; D003911:Dextrans; D066298:In Vitro Techniques; D008955:Models, Cardiovascular; D011108:Polymers; D012995:Solubility; D013499:Surface Properties
D001672:Biocompatible Materials; D003911:Dextrans; D011108:Polymers