title: Kinetics of carboxylmethylation of the charge isoforms of myelin basic protein by protein methyltransferase II.
abstract: The charge isoforms (C1-C5) of bovine myelin basic protein (MBP) were used as substrates for the rat brain enzyme protein carboxylmethyltransferase (PM II). The objective of these experiments was to ascertain whether the kinetic behavior of the MBP isoforms reflected differences in the structures of this molecular family. Initial velocity plots as a function of the MBP-isoform concentration showed significant differences (p less than 0.05) among the assayed isoforms except for isoforms C2 and C4. Under the conditions of our experiment all the curves exhibited a consistent sigmoidicity. The kinetic data were best fitted by a model, previously described for the enzyme D-beta-hydroxybutyrate dehydrogenase, in which two independent sites must be randomly occupied before any catalytic activity can occur. This mechanism is substantially different from that proposed by other investigators for similar PM II enzymes and other substrates. The differences in the rates of isoform carboxylmethylation are largely accounted for by the different apparent dissociation constants Ks and is explained on the basis of inherent structural differences among the charge isoforms.
authors: Biophysics Research Division, University of Michigan, Ann Arbor 48109-2099.
authors: C A
authors: CA
authors: Caamaño
authors: J M
authors: JM
authors: Azcurra
authors: O Z
authors: OZ
authors: Sellinger
authors: R
authors: R
authors: Zand
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000378:metabolism; D002417:Cattle; D003600:Cytosol / Q000201:enzymology; D007700:Kinetics; D008745:Methylation; D004676:Myelin Basic Protein / Q000302:isolation & purification / Q000378:metabolism*; D011496:Protein Methyltransferases / Q000378:metabolism*; D011497:Protein O-Methyltransferase / Q000378:metabolism*; D051381:Rats; D013116:Spinal Cord / Q000378:metabolism
chemical_list: D004676:Myelin Basic Protein; D011496:Protein Methyltransferases; D011497:Protein O-Methyltransferase