title: Characterization of antigenic variants of tick-borne encephalitis virus selected with neutralizing monoclonal antibodies.
abstract: Antigenic variants of tick-borne encephalitis virus were selected by the use of six neutralizing monoclonal antibodies (MAbs), each defining a different epitope of the envelope glycoprotein E. These variants were characterized with respect to antigenic changes by analysing the binding of each of 18 precisely mapped MAbs in ELISA and haemagglutination inhibition tests. The results yielded information about interrelations between epitopes exceeding that obtained previously from competitive binding studies. In addition, variants were tested for their specific haemagglutination activities, which revealed a significant reduction of this functional activity in one of the variants.
authors: Institute of Virology, University of Vienna, Austria.
authors: H
authors: H
authors: Holzmann
authors: C W
authors: CW
authors: Mandl
authors: F
authors: F
authors: Guirakhoo
authors: F X
authors: FX
authors: Heinz
authors: C
authors: C
authors: Kunz
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000940:Antigenic Variation; D000956:Antigens, Viral / Q000276:immunology*; D001667:Binding, Competitive; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D006385:Hemagglutination Inhibition Tests; D006387:Hemagglutination, Viral; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008562:Membrane Glycoproteins / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes; D008562:Membrane Glycoproteins