Preparative separation of poliovirus structural polypeptides by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, copper staining and electroelution, and induction of monospecific antisera.
Viral polypeptides were prepared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by copper staining and electroelution from gel slices. Poliovirus capsid polypeptide VP 1 isolated by this procedure induced monospecific antibodies in rabbits, i.e., antisera reacting only with the homologous polypeptide. Our results demonstrate the applicability of the described copper staining method as a rapid visualization step for preparing viral proteins after SDS-PAGE.
Tessmer
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U
Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Universität Hamburg, Federal Republic of Germany.
Dernick
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R
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral / Q000276:immunology / Q000302:isolation & purification*; D002213:Capsid / Q000276:immunology / Q000302:isolation & purification*; D003300:Copper; D004591:Electrophoresis, Polyacrylamide Gel; D017955:Poliovirus / Q000032:analysis*; D011817:Rabbits; D012967:Sodium Dodecyl Sulfate; D013194:Staining and Labeling
D000914:Antibodies, Viral; D000956:Antigens, Viral; D012967:Sodium Dodecyl Sulfate; D003300:Copper