Cloning of Plasmodium yoelii genes expressing three different sporozoite-specific antigens.
Genomic DNA isolated from Plasmodium yoelii (strain #17XNL) was prepared by partial digestion and cloned in Escherichia coli TB-1 with pUC18 plasmid. Antigen-producing recombinants were detected by a battery of monoclonal antibodies against antigens of the sporozoite stages. Four clones producing stage-specific sporozoite antigens were identified. One produced P. yoelii circumsporozoite protein, and three produced other P. yoelii sporozoite antigens.
Wortman
A
A
Infectious Diseases Program Center, Naval Medical Research Institute, Bethesda, Maryland 20814-5055.
Rogers
P
P
Charoenvit
Y
Y
McDermott
A
A
Leef
M
M
Sedegah
M
M
Beaudoin
R L
RL
D000818:Animals; D000953:Antigens, Protozoan / Q000096:biosynthesis / Q000235:genetics*; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D000939:Epitopes / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010967:Plasmodium yoelii / Q000235:genetics* / Q000276:immunology; D015183:Restriction Mapping
D000953:Antigens, Protozoan; D000939:Epitopes; D004247:DNA