A Purkinje cell differentiation marker shows a partial DNA sequence homology to the cellular sis/PDGF2 gene.
To search for genes involved in determining the morphology of individual neuronal types, a cDNA library was constructed from postnatal day 13 mouse cerebellum. From this library, 2 clones, L7 and L19, were isolated by a differential hybridization procedure and shown by in situ hybridization to be Purkinje cell-specific within the cerebellum. Both RNAs appear between postnatal days 4 and 8 and continue into adulthood, coinciding with terminal differentiation of the Purkinje cells. L7 seems to be expressed exclusively in the cerebellum, whereas L19 is expressed throughout the brain. Consistent with the RNA localization, L7 protein is found only in the cerebellum and is confined to the Purkinje cells. The L7 amino acid sequence has been deduced from the cDNA sequence, and a pseudo-repeat within the L7 protein sequence is homologous to the amino acids sequence in the primary translation product of the gene for human sis/PDGF.
Oberdick
J
J
Department of Biological Sciences, Columbia University, New York, New York 10027.
Levinthal
F
F
Levinthal
C
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002454:Cell Differentiation; D002531:Cerebellum / Q000502:physiology; D004247:DNA / Q000235:genetics*; D015342:DNA Probes; D015723:Gene Library; D005796:Genes; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D009928:Organ Specificity; D010982:Platelet-Derived Growth Factor / Q000235:genetics*; D011689:Purkinje Cells / Q000502:physiology*; D012313:RNA / Q000235:genetics* / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid
D015342:DNA Probes; D009419:Nerve Tissue Proteins; D010982:Platelet-Derived Growth Factor; D012313:RNA; D004247:DNA