Effects of MPP+ on the release of serotonin and 5-hydroxyindoleacetic acid from rat striatum in vivo.
A procedure utilizing brain dialysis was employed to investigate the effects of 1-methyl-4-phenylpyridinium ion (MPP+), the major metabolite of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), on the in vivo release of 5-hydroxytryptamine (5-HT) from the rat striatum. MPP+ (10(-5)-10(-2) M) was administered through the microdialysis probe and dialysates were collected and assayed for monoamines and their metabolites by a high performance liquid chromatography coupled with an electrochemical detector (HPLC-EC). In addition to a dopamine (DA) releasing action, MPP+ caused a cumulatively dose-dependent increase in the release of 5-HT while concomitantly producing a decrease in the efflux of 5-hydroxyindoleacetic acid (5-HIAA). This MPP+-induced increase in the 5-HT release, may play an important role in the acute 5-HT syndrome seen in animals after MPTP.
Miyake
H
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Laboratory of Cerebral Metabolism, National Institute of Mental Health, Bethesda, MD 20892.
Chiueh
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CC
D015655:1-Methyl-4-phenylpyridinium / Q000494:pharmacology*; D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000758:Anesthesia; D000818:Animals; D015306:Biogenic Monoamines / Q000378:metabolism; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006719:Homovanillic Acid / Q000378:metabolism; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism*; D008297:Male; D009498:Neurotoxins / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D012701:Serotonin / Q000378:metabolism*
D015306:Biogenic Monoamines; D009498:Neurotoxins; D015102:3,4-Dihydroxyphenylacetic Acid; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D015655:1-Methyl-4-phenylpyridinium; D006719:Homovanillic Acid