Cross-reactive antigenic determinants present on different Plasmodium falciparum blood-stage antigens.
A gene encoding a previously undescribed antigen of Plasmodium falciparum has been isolated from a genomic expression library by use of a pool of human immune sera. Northern blot analysis indicated that the gene is expressed at the late stages of the intra-erythrocytic cycle. This antigen, 332, contains a series of degenerated amino acid repeats. Human antibodies affinity-purified on the 332 recombinant antigen reacted with a family of parasite proteins that are products of different genes. We identified antigens 11.1 and Pf155-RESA as members of this family and confirmed, using a human monoclonal antibody, the presence of cross-reacting determinants. The sequences of these antigens also share some structural homologies. The significance of this family of blood-stage antigens is discussed.
Mattei
D
D
Unité de Parasitologie Expérimentale, Institut Pasteur, Paris, France.
Berzins
K
K
Wahlgren
M
M
Udomsangpetch
R
R
Perlmann
P
P
Griesser
H W
HW
Scherf
A
A
Müller-Hill
B
B
Bonnefoy
S
S
Guillotte
M
M
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003429:Cross Reactions; D004247:DNA / Q000235:genetics; D000939:Epitopes / Q000235:genetics; D004912:Erythrocytes / Q000469:parasitology; D006801:Humans; D008288:Malaria / Q000097:blood / Q000276:immunology / Q000469:parasitology; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000235:genetics / Q000254:growth & development / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D000939:Epitopes; D004247:DNA