Three dimensional structure of a functional internal image.
An anti-idiotypic antibody has been characterized that forms a functional internal image of the cell-attachment site of reovirus type 3. This antibody shares sequence similarity with the reovirus type 3 cell attachment protein. Studies utilizing synthetic peptides have shown that the region of sequence similarity corresponds to the cell-attachment site of the virus and the antibody. Studies are described demonstrating that these peptides possess biological activity similar to that of the virus and the antibody. Three-dimensional models of the cell-attachment site of the virus are presented and compared to a similar model for the antibody. These studies indicate that anti-idiotypic antibodies can be utilized to understand the three-dimensional basis of ligand - receptor interactions, and can lead to the development of novel biologically active substances.
Williams
W V
WV
Department of Medicine, University of Pennsylvania, School of Medicine, Philadelphia.
Guy
H R
HR
Weiner
D B
DB
Greene
M I
MI
D000595:Amino Acid Sequence; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000378:metabolism*; D001666:Binding Sites, Antibody; D000939:Epitopes / Q000276:immunology*; D006389:Hemagglutinins, Viral / Q000276:immunology*; D007114:Immunization; D008213:Lymphocyte Activation; D012089:Mammalian orthoreovirus 3 / Q000276:immunology*; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology; D011487:Protein Conformation; D012087:Reoviridae / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D006389:Hemagglutinins, Viral; D010455:Peptides