Occurrence of an anti-peplomycin IgE antibody cross-reacting with bleomycin in a patient with cervical uterine cancer.
An allergic reaction to peplomycin was observed in a patient with cervical uterine cancer who had previously been treated with peplomycin. A positive Prausnitz-Küstner test and its elimination after heat treatment of the serum showed the production of anti-peplomycin IgE antibody. Peplomycin was coupled to a paper disc and a sensitive radioallergosorbent test for peplomycin was developed to quantitate the antibody. Patient serum IgE and IgG were purified by DE52 column chromatography; the IgE fraction contained binding activity to peplomycin. A competition test revealed that the antibody bound to both peplomycin and bleomycin. DNA, RNA, and mononucleotides had no effect on antibody binding, but the antibody inhibited peplomycin's activity.
Sakura
H
H
Department of Obstetrics and Gynecology, Hamamatsu Medical Center, Japan.
Fujii
T
T
Ohashi
T
T
Tsuruta
S
S
D000368:Aged; D000918:Antibody Specificity; D001761:Bleomycin / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000627:therapeutic use; D003131:Combined Modality Therapy; D003429:Cross Reactions; D004342:Drug Hypersensitivity / Q000276:immunology*; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis; D007428:Intradermal Tests; D017663:Peplomycin; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy / Q000276:immunology*
D007074:Immunoglobulin G; D001761:Bleomycin; D007073:Immunoglobulin E; D017663:Peplomycin