Electrophysiological expression of endothelin and angiotensin receptors in Xenopus oocytes injected with rat heart mRNA.
Functional endothelin and angiotensin receptors have been expressed in Xenopus oocyte following the microinjection of rat heart mRNA. Under voltage clamp conditions, application of these peptides clearly induced oscillatory Ca2+-activated chloride currents in a dose-dependent manner. In addition, no direct modulation of expressed or native cardiac Ca channels was observed.
Lory
P
P
Centre de Recherche de Biochimie Macromoléculaire, UPR 8402 CNRS, U249 INSERM, Montpellier, France.
Richard
S
S
Rassendren
F A
FA
Tiaho
F
F
Nargeot
J
J
D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D018118:Chloride Channels; D002712:Chlorides / Q000502:physiology*; D004553:Electric Conductivity; D016232:Endothelins; D004730:Endothelium, Vascular / Q000502:physiology; D005260:Female; D015870:Gene Expression; D006352:Heart Ventricles / Q000187:drug effects; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D008565:Membrane Proteins / Q000502:physiology*; D008845:Microinjections; D009206:Myocardium / Q000378:metabolism*; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D010455:Peptides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D011945:Receptors, Angiotensin / Q000235:genetics* / Q000502:physiology; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000502:physiology; D017466:Receptors, Endothelin; D016276:Ventricular Function; D014981:Xenopus
D018118:Chloride Channels; D002712:Chlorides; D016232:Endothelins; D007473:Ion Channels; D008565:Membrane Proteins; D010455:Peptides; D012333:RNA, Messenger; D011945:Receptors, Angiotensin; D011956:Receptors, Cell Surface; D017466:Receptors, Endothelin; D000804:Angiotensin II