Interrelationship of number of glucose carriers and intracellular phosphoribosyl diphosphate level of Ehrlich ascites tumor cells in vitro.
The intracellular phosphoribosyl diphosphate (prpp) levels of Ehrlich ascites tumor cells increased during glucose supplementation and decreased during glucose deprivation, while the numbers of glucose carriers as determined by glucose-reversible cytochalasin-B binding changed in an opposite manner relating to the extracellular glucose concentrations and the intracellular prpp levels of Ehrlich ascites tumor cells in vitro. Incubation of cells with hypoxanthine or 2,4-dinitrophenol lowered the intracellular prpp levels and resulted in an increase in numbers of glucose carriers.
Choy
Y M
YM
Department of Biochemistry, Chinese University of Hong Kong, Shatin.
Lam
W P
WP
Fung
K P
KP
D019297:2,4-Dinitrophenol; D000818:Animals; D001665:Binding Sites; D001692:Biological Transport / Q000187:drug effects; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D003571:Cytochalasin B / Q000378:metabolism; D004140:Dinitrophenols / Q000494:pharmacology; D005110:Extracellular Space; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000494:pharmacology; D066298:In Vitro Techniques; D010428:Pentosephosphates / Q000378:metabolism*; D010754:Phosphoribosyl Pyrophosphate / Q000378:metabolism*
D004140:Dinitrophenols; D007042:Hypoxanthines; D010428:Pentosephosphates; D019271:Hypoxanthine; D003571:Cytochalasin B; D010754:Phosphoribosyl Pyrophosphate; D005947:Glucose; D019297:2,4-Dinitrophenol