Rabies virus-specific human T cell clones provide help for an in vitro antibody response against neutralizing antibody-inducing determinants of the viral glycoprotein.
Human T cell clones were prepared from peripheral blood mononuclear cells from a vaccinated human donor and kept in culture in the presence of rabies virus antigen and growth factors. Phenotypic analysis of the T cell clones revealed expression of the CD3 and CD4 cell surface markers, but not of CD8, consistent with a phenotype of helper/inducer T cells. The rabies virus specificity of the T cell clones was established by virus-specific proliferation in response to the rabies virus Pitman-Moore strain (PM) produced in three different cell substrates. The clones also responded to the rabies virus strains Evelyn-Rokitnicki-Abelseth (ERA) and challenge virus standard (CVS), but not to the rabies virus-related Mokola and Duvenhage-6 virus strains. Proliferative responses of T cell clones required rabies virus antigen to be presented by autologous antigen-presenting cells in association with HLA class II molecules. When cultured with rabies virus antigen, but in the absence of growth factors, some of the T cell clones provided help for an antibody response of rabies virus immune B lymphocytes. Analysis of culture supernatant fluids showed that at least a part of this antibody response was directed against neutralizing antibody-inducing determinants of the viral glycoprotein.
Bunschoten
H
H
National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
Klapmuts
R J
RJ
Claassen
I J
IJ
Reyneveld
S D
SD
Osterhaus
A D
AD
Uytdehaag
F G
FG
D000914:Antibodies, Viral / Q000032:analysis / Q000096:biosynthesis*; D000956:Antigens, Viral / Q000276:immunology*; D001667:Binding, Competitive; D002999:Clone Cells / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D008285:Major Histocompatibility Complex; D009500:Neutralization Tests; D010641:Phenotype; D011819:Rabies Vaccines / Q000008:administration & dosage / Q000276:immunology; D011820:Rabies virus / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D006023:Glycoproteins; D011819:Rabies Vaccines; D014764:Viral Proteins