title: Active in situ immune complex glomerulonephritis using the hapten-carrier system: role of epitope density in cationic antigens.
abstract: The role of epitope density in cationic antigen was investigated in an active model of in situ immune complex glomerulonephritis (ICGN) using the hapten-carrier system. Trinitrophenol (TNP) was conjugated with variable density to cationic human immunoglobulin (C-HIgG) to yield TNP6.2-C-HIgG (low-valency antigen) and TNP31.3-C-HIgG (high-valency antigen). In rats preimmunized with TNP17.3-bovine serum albumin (BSA), endocapillary proliferative GN with proteinuria developed in rats receiving high-valency antigen. In contrast, no significant abnormalities in renal histology or urinalysis were observed when a low-valency antigen was injected. These results indicate that glomerular injury produced by hapten-specific immune reaction is affected by the number of haptenic groups conjugated to the carrier molecule (epitope density) in active in situ ICGN.
authors: Department of Immunology, Niigata University School of Medicine, Japan.
authors: S
authors: S
authors: Kagami
authors: M
authors: M
authors: Miyao
authors: F
authors: F
authors: Shimizu
authors: T
authors: T
authors: Oite
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis; D000939:Epitopes / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D005921:Glomerulonephritis / Q000276:immunology* / Q000473:pathology; D006241:Haptens; D007105:Immune Complex Diseases / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D007668:Kidney / Q000473:pathology; D008297:Male; D011507:Proteinuria; D051381:Rats; D011919:Rats, Inbred Strains; D014301:Trinitrobenzenes / Q000276:immunology
chemical_list: D000906:Antibodies; D000939:Epitopes; D006241:Haptens; D007074:Immunoglobulin G; D014301:Trinitrobenzenes