A common epitope on human myelin basic protein and the human T lymphocyte CD3 molecule.
In this report, we describe for the first time an epitope common to human myelin basic protein (H.MBP), a structural component of central nervous system myelin, and T lymphocyte CD3, an activation molecule important in signal transduction. This cross-reactive determinant was recognized by a murine mAb WW.B1, which was raised against H.MBP. WW.B1 recognized PBMC and the Jurkat T leukemic cell line, immunoprecipitated both H.MBP and a complex indistinguishable from CD3, and possessed the same biologic properties--induction of T lymphocyte proliferation and inhibition of CTL function--as commercially available anti-CD3 antibodies. It is likely, however, that the epitope recognized by WW.B1 is distinct from those recognized by the anti-CD3 mAb OKT3 and anti-Leu-4. Although the biologic importance of this common determinant awaits further clarification, it is conceivable that autoimmunization to MBP could induce similar immunoregulatory antibody specificities.
Zhang
J W
JW
Department of Immunology, Dr. L. Willems Institute, Diepenbeek, Belgium.
Weber
W E
WE
Borst
J
J
Vandenbark
A A
AA
Raus
J C
JC
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D001666:Binding Sites, Antibody; D017252:CD3 Complex; D000939:Epitopes / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007202:Indicators and Reagents; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D004676:Myelin Basic Protein / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D011233:Precipitin Tests; D011948:Receptors, Antigen, T-Cell / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D013601:T-Lymphocytes / Q000032:analysis* / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D000939:Epitopes; D007202:Indicators and Reagents; D004676:Myelin Basic Protein; D011948:Receptors, Antigen, T-Cell