Experimental demonstration of lateral cohesion in a layer of adsorbed protein and in layers of gold-antibody complexes bound to surface immobilised antigen.
The spatial distribution of ferritin molecules adsorbed on quartz surfaces and the spatial distribution of antibody-coated colloidal gold particles over an antigen-coated surface was studied by electron microscopy. Ferritin molecules were found to initially adsorb in small clusters at 2.5 X 10(9) sites/cm2. The initial nucleation was followed by growth of the clusters. Gold antibody complexes were initially bound to the antigen-coated surface as single particles, and formation of clusters was a secondary event at higher particle densities. The results indicate that lateral cohesion between macromolecules may play a role in the stability of adsorbed layers of protein and surface immobilised antigen-antibody complexes.
Nygren
H
H
Department of Histology, University of Gothenburg, Sweden.
D000818:Animals; D000911:Antibodies, Monoclonal; D000915:Antibody Affinity; D000936:Antigen-Antibody Complex; D001666:Binding Sites, Antibody; D000939:Epitopes / Q000276:immunology; D005293:Ferritins / Q000276:immunology / Q000648:ultrastructure*; D006046:Gold; D006736:Horses; D007163:Immunosorbent Techniques
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000939:Epitopes; D006046:Gold; D005293:Ferritins