A brush method to harvest cells from the nasal mucosa for microscopic and biochemical analysis.
A method is described for the sampling of epithelial cells and other effector cells from the human airway mucosa for structural and biochemical analysis. The cell samples are obtained from the nasal mucosa using a small nylon brush which is rotated over the epithelium and soaked and shaken in a small volume of a balanced salt solution. Morphological evaluation using light microscopy and transmission electron microscopy revealed excellently preserved cytological detail. In asymptomatic individuals the cells harvested were as follows: 45 +/- 5.9% (mean +/- SEM) epithelial cells, 38 +/- 7.1% granulocytes, 16 +/- 2.3% large mononuclear cells (monocytes), and 1.3 +/- 2.3% eosinophils. Repeated measurements in the same individual revealed a coefficient of variation of the order of 40% for the proportions of cells harvested. In comparison with nasal airway lavage, a higher proportion of epithelial cells and monocytes were obtained with the brush method. The cells harvested could also be used for biochemical analysis. The histamine content of the cell pellets was found to be strongly correlated with the mast cell count (r = 0.93) and was estimated to about 10 pg/cell, which is higher than previously reported for mast cells obtained from human lung tissue dispersed by an enzymatic method. The present method appears to be appropriate for the study of cellular events in the nasal mucosal epithelium.
Pipkorn
U
U
Department of Otorhinolaryngology, University Hospital, Lund, Sweden.
Karlsson
G
G
Enerbäck
L
L
D000328:Adult; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D004847:Epithelial Cells; D004848:Epithelium / Q000032:analysis / Q000648:ultrastructure; D006636:Histamine Release; D006651:Histocytochemistry / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D009297:Nasal Mucosa / Q000032:analysis / Q000166:cytology* / Q000648:ultrastructure; D006255:Rhinitis, Allergic, Seasonal / Q000378:metabolism / Q000473:pathology; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods