Establishment of a feline T-lymphoblastoid cell line highly sensitive for replication of feline immunodeficiency virus.
Interleukin-2 dependent feline T-lymphoblastoid cells designated as MYA-1 cells were established. The cells were free from exogenous retroviruses and sensitive for replication of feline immunodeficiency virus (FIV). FIV can grow more efficiently in MYA-1 cells than in feline primary peripheral blood mononuclear cells. This line of cells will be useful not only for isolation and propagation of FIV, but also for further investigation of properties of FIV.
Miyazawa
T
T
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Furuya
T
T
Itagaki
S
S
Tohya
Y
Y
Takahashi
E
E
Mikami
T
T
D000818:Animals; D002415:Cats; D002460:Cell Line; D008297:Male; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013047:Specific Pathogen-Free Organisms; D013601:T-Lymphocytes / Q000382:microbiology*; D014776:Virus Cultivation; D014779:Virus Replication; D014790:Visna-maedi virus / Q000254:growth & development / Q000502:physiology*
D012194:RNA-Directed DNA Polymerase