Defective transport of hemagglutinin-neuraminidase glycoprotein of bovine parainfluenza-3 virus in interferon treated cell.
A defective transport of bovine parainfluenza-3 virus (PI-3V) hemagglutinin-neuraminidase (HN) glycoprotein was evidenced in interferon (IFN)-treated bovine turbinate (BTu) cells. Indirect immunofluorescence performed with monoclonal antibody to PI-3 HN glycoprotein demonstrated accumulation of this protein in the perinuclear cytoplasm of IFN-treated cells. Untreated, infected control cells had a generalized widespread fluorescence. Unfixed control cells showed a uniform surface fluorescence in contrast to a few specs of fluorescence on the plasma membrane of IFN-treated cells. Electron microscopic localization of HN protein was done by immuno-gold ultrastructural cytochemistry. Untreated cells had uniform gold label on the plasma membrane and around the budding virus particles with no label in the cytoplasm. In IFN-treated cells, however, there was an accumulation of gold particles in the cytoplasm with only a few particles on the cell surface. Quantitative analysis of HN protein on the cell surface by solid phase radioimmune-assay revealed a greater amount of this protein on the surface of control cells, than those on the IFN-treated cells.
Panigrahi
P
P
Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park.
Mohanty
S B
SB
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001692:Biological Transport; D002417:Cattle; D002451:Cell Compartmentation; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D015707:HN Protein / Q000276:immunology / Q000378:metabolism*; D007150:Immunohistochemistry; D007372:Interferons / Q000494:pharmacology*; D010224:Parainfluenza Virus 3, Human / Q000378:metabolism*; D018108:Respirovirus / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D015707:HN Protein; D007372:Interferons