Application of high-performance liquid chromatography for the study of the microheterogeneity changes of mouse alpha-fetoprotein in fetal development.
Changes in the microheterogeneity of mouse alpha-fetoprotein (MAFP) during fetal development were investigated by high-performance liquid chromatography (HPLC). A total of six distinct isoforms (Iso-1, Iso-2, Iso-3, Iso-4, Iso-5, Iso-6) of the heterogeneous MAFP were resolved from mouse amniotic fluid (MAF). Analysis of MAF collected at various times revealed that these isoforms were accumulated at different stages of the fetal development.
Wong
L T
LT
Department of Biochemistry, University of Toronto, Ontario, Canada.
Lu
C Y
CY
Tinker
D O
DO
Hsia
J C
JC
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005314:Embryonic and Fetal Development; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D000509:alpha-Fetoproteins / Q000032:analysis*
D000509:alpha-Fetoproteins