Bleomycin-stimulated hamster alveolar macrophages release interleukin-1.
The capacity of alveolar macrophages (AM) of bleomycin-instilled hamsters to proliferate mouse thymocytes (interleukin-1 activity) and hamster fibroblasts (fibroblast proliferation (FP) activity was studied. Using bleomycin-instilled hamsters, the FP activity of AM culture supernatants was increased significantly on days 1, 5, and 10 after instillation of bleomycin. The interleukin-1 (IL-1) activity, however, was increased significantly on day 1 only as compared with saline-treated hamsters. Next, normal AM were stimulated in vitro by bleomycin. After being fractionated by chromatography, their culture supernatant showed IL-1 activity, which also indicated FP activity. These results suggest that bleomycin directly stimulates AM to release IL-1 in the fibrogenic responses.
Suwabe
A
A
First Department of Intenral Medicine, Yamagata University School of Medicine, Japan.
Takahashi
K
K
Yasui
S
S
Arai
S
S
Sendo
F
F
D000818:Animals; D001761:Bleomycin; D002455:Cell Division; D006224:Cricetinae; D005347:Fibroblasts / Q000502:physiology; D006133:Growth Substances / Q000378:metabolism; D066298:In Vitro Techniques; D036341:Intercellular Signaling Peptides and Proteins; D007375:Interleukin-1 / Q000378:metabolism*; D008171:Lung Diseases / Q000139:chemically induced / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D008297:Male; D008647:Mesocricetus; D051379:Mice; D008809:Mice, Inbred C3H; D010455:Peptides; D011650:Pulmonary Alveoli / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*
D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D007375:Interleukin-1; D010455:Peptides; C042586:alveolar macrophage growth factor; D001761:Bleomycin