Primary structure and functional expression from complementary DNA of the rod photoreceptor cyclic GMP-gated channel.
The complete amino-acid sequence of the cyclic GMP-gated channel from bovine retinal rod photoreceptors, deduced by cloning and sequencing its complementary DNA, shows that the protein contains several putative transmembrane segments, followed by a region that is similar to the cyclic GMP-binding domains of cyclic GMP-dependent protein kinase. Expression of the complementary DNA produces cyclic GMP-gated channel activity in Xenopus oocytes.
Kaupp
U B
UB
Department of Medical Chemistry, Kyoto University Faculty of Medicine, Japan.
Niidome
T
T
Tanabe
T
T
Terada
S
S
Bönigk
W
W
Stühmer
W
W
Cook
N J
NJ
Kangawa
K
K
Matsuo
H
H
Hirose
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D006152:Cyclic GMP / Q000502:physiology*; D054815:Cyclic Nucleotide-Gated Cation Channels; D004247:DNA / Q000235:genetics; D005136:Eye Proteins / Q000235:genetics*; D066298:In Vitro Techniques; D015640:Ion Channel Gating; D007473:Ion Channels / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D011487:Protein Conformation; D012333:RNA, Messenger / Q000235:genetics; D012374:Rod Cell Outer Segment; D012995:Solubility; D014982:Xenopus laevis
D054815:Cyclic Nucleotide-Gated Cation Channels; D005136:Eye Proteins; D007473:Ion Channels; D008565:Membrane Proteins; D012333:RNA, Messenger; D004247:DNA; D006152:Cyclic GMP