Accelerated immunogold silver and immunoperoxidase staining of paraffin sections with the use of microwave irradiation. Factors influencing results.
With the use of microwave irradiation, incubation times for the immunotechniques used in immunomicroscopy can be shortened significantly. The authors found that factors influencing results are irradiation time, power output, and presence of gold label. Precise temperature control is essential, and techniques to achieve this are discussed. For beta-human chorionic gonadotropin (beta-HCG), the results with the immunoperoxidase technique were inferior to those of the conventional technique, and with the immunogold silver technique an enhancement of labeling results was achieved. Immunogold silver staining for beta-HCG on paraffin sections can be performed with the use of microwave irradiation within one hour.
Boon
M E
ME
Leiden Cytology and Pathology Laboratory, The Netherlands.
Kok
L P
LP
Moorlag
H E
HE
Suurmeijer
A J
AJ
D006652:Histological Techniques; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008872:Microwaves; D010232:Paraffin; D013194:Staining and Labeling / Q000379:methods*; D013696:Temperature
D010232:Paraffin