Specificity of antibodies to sea anemone toxin III and immunogenicity of the pharmacological site of anemone and scorpion toxins.
Toxin III (ATX III) of the sea anemone (Anemonia sulcata) is a polypeptide containing 27 amino acid residues. It has no sequence similarity with other toxins (ATX I and II) from the same species, or with scorpion toxins, although they apparently act in a similar manner by prolonging action potentials. The specificity of ATX III antibodies was characterized using ATX III, ATX I, native and chemically modified ATX II, and scorpion alpha-toxins. The results obtained suggest that a region of ATX III, partially or totally overlapping the pharmacological site shared with ATX I and ATX II, is immunogenic. It includes a guanidino and at least two carboxylate groups. The corresponding region is not immunogenic in ATX I and ATX II. Anti-(ATX III) antibodies recognize the similar regions of ATX I and ATX II and apparently do not recognize scorpion toxins.
Bahraoui
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EM
Laboratoire de Biochimie, Unité associée 1179 du Centre National de la Recherche Scientifique, Faculté de Médecine Nord, Marseille, France.
el Ayeb
M
M
Granier
C
C
Beress
L
L
Rochat
H
H
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D001666:Binding Sites, Antibody; D003063:Cnidaria / Q000276:immunology*; D003064:Cnidarian Venoms / Q000276:immunology* / Q000494:pharmacology; D003429:Cross Reactions; D000939:Epitopes; D007106:Immune Sera / Q000032:analysis; D007074:Immunoglobulin G / Q000302:isolation & purification; D009500:Neutralization Tests; D011863:Radioimmunoassay; D012604:Scorpion Venoms / Q000276:immunology* / Q000494:pharmacology; D012615:Sea Anemones / Q000276:immunology*
D003064:Cnidarian Venoms; D000939:Epitopes; D007106:Immune Sera; D007074:Immunoglobulin G; D012604:Scorpion Venoms; C028800:toxin II (Anemonia sulcata); C032104:toxin III (Anemonia sulcata)