Comparative study of 1.5-hour and 48-hour homologous passive cutaneous anaphylaxis in the mouse ear.
To characterize passive cutaneous anaphylaxis (PCA) in the mouse ear, reactions caused by non-heated and heated antiserum were compared to those caused by monoclonal immunoglobulin E antibody (mc-IgE) and monoclonal immunoglobulin G1 antibody (mc-IgG1). Heat treatment at 56 degrees C did not alter the activity of antiserum or mc-IgG1 to elicit the 1.5-h PCA. The 1.5-h PCA mediated by mc-IgE and 48-h PCA's mediated by both antiserum and mc-IgE were abrogated almost completely by heating at 56 degrees C for 2 h. The 48-h PCA was not elicited by mc-IgG1. The sensitized state persisted at least for 7 d when mice were sensitized with non-heated antiserum or mc-IgE. In contrast, the sensitized state disappeared rapidly in cases of heated antiserum and mc-IgG1. In 48-h PCA's mediated by antiserum and mc-IgE, extravasated dye in the ear was detected 5 min after challenge and the reaction was terminated in 15 min. In contrast, in the 1.5-h PCA mediated by heated antiserum, the accumulation of dye in the ear was delayed slightly when compared to the 48-h PCA. In mc-IgG1-mediated 1.5-h PCA, the delay was significant. Both 1.5-h PCA's mediated by heated antiserum and mc-IgG1, and the 48-h PCA's mediated by antiserum and mc-IgE were not observed in WBB6 F1-W/Wv mice, which lack mast cells. All these PCA's were inhibited equally by tranilast, an inhibitor of mediator release from mast cells.(ABSTRACT TRUNCATED AT 250 WORDS)
Inagaki
N
N
Department of Pharmacology, Gifu Pharmaceutical University, Japan.
Nagai
H
H
Nakatomi
I
I
Koda
A
A
D000818:Animals; D000911:Antibodies, Monoclonal; D004396:Coloring Agents; D004423:Ear; D005260:Female; D006358:Hot Temperature; D007106:Immune Sera; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D010323:Passive Cutaneous Anaphylaxis; D013997:Time Factors; D062367:ortho-Aminobenzoates
D000911:Antibodies, Monoclonal; D004396:Coloring Agents; D007106:Immune Sera; D062367:ortho-Aminobenzoates; C012293:tranilast