Maxisorp RAST. A sensitive method for detection of antigen-specific human IgE in culture fluids.
For determination of allergen-specific IgE in cell culture supernatants and other highly diluted IgE preparations a radioallergosorbent test (RAST) based on high adsorption polystyrene test tubes has been developed ("Maxisorp RAST"). Cladosporium herbarum extract was used as a model allergen but timothy grass pollen, house dust mite and dog dander showed similar results. The test showed specificities of both allergen and immunoglobulin isotype and significant correlations (r = 0.67-0.88) with established RAST procedures were found. Based on immunosorbent-purified allergen-specific IgE the estimated sensitivity was within the order of 150-300 pg allergen-specific IgE per ml. The within-assay variation was 4-9% and the inter-assay-variation 17-29%. The Maxisorp RAST is useful as an inhibition assay for quantitating allergenic activity down to 0.1 biological units/ml of allergen extracts.
Poulsen
L K
LK
Laboratory of Medical Allergology, Medical Dept. TTA 7542, State University Hospital, Copenhagen, Denmark.
Pedersen
M F
MF
Malling
H J
HJ
Søndergaard
I
I
Weeke
B
B
D000485:Allergens / Q000592:standards; D000946:Antigens, Fungal; D002963:Cladosporium / Q000276:immunology; D000939:Epitopes; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis*; D011852:Radioallergosorbent Test / Q000379:methods*
D000485:Allergens; D000946:Antigens, Fungal; D000939:Epitopes; D007073:Immunoglobulin E