Perifusion of isolated rat pancreatic acini: carbamylcholine-induced biphasic amylase release.
To solve many problems in other in-vitro methods such as perfusion and static incubation, perifusion of isolated rat pancreatic acini was established. Continuous stimulation by carbamylcholine induced a biphasic secretory response, a sharp initial phase and a slow-varying second phase. The junction of these two phases occurred at almost the same time (in about 7 min of stimulation) at any concentration of carbamylcholine. The slow-rising former part of a second phase indicated that a biphasic pattern is produced by overlapping of two kinds of real phases and the beginning of a real second phase occurs at the time of the junction. The concentration dependence of amylase release showed a bell-shaped pattern and the maximal amylase release at an optimal concentration (10(-6)M) was 5.43 +/- 0.24% total/30 min. The sustained latter part of a second phase showed a slow decline at optimal or less concentrations but a plateau at supraoptimal concentrations.
Nagai
M
M
First Department of Internal Medicine, University of Tokyo Fuculty of Medicine, Japan.
Oka
H
H
D000681:Amylases / Q000378:metabolism*; D000818:Animals; D002217:Carbachol / Q000494:pharmacology*; D008297:Male; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D013268:Stimulation, Chemical
D002217:Carbachol; D000681:Amylases