title: Changes in cell surface anionogenic groups during differentiation of Herpetomonas samuelpessoai mediated by dimethylsulfoxide.
abstract: The surface anionic groups of untreated or dimethyl sulfoxide (DMSO)-treated Herpetomonas samuelpessoai cells were analyzed by cell electrophoresis, ultrastructural cytochemistry, and identification of sialic acids using thin-layer chromatography. Differentiation of H. samuelpessoai induced by DMSO treatment caused a significant increase in the net negative surface charge. In flagellates exposed to DMSO, more cationized ferritin, colloidal iron hydroxide, and sendai virus particles bound to the cell surface. Treatment of both untreated and DMSO-treated flagellates with neuraminidase decreased markedly the EPM of cells to the cathodic pole. These findings suggest that sialic acid residues are the major anionogenic groups exposed on the surface of H. samuelpessoai. Thin-layer chromatography showed that N-acetyl and N,O-diacylneuraminic acids, in equal proportions, were present in H. samuelpessoai. However, N-acetylneuraminic acid predominates in DMSO-treated cells.
authors: Instituto de Microbiologia, UFRJ, Rio de Janeiro, Brasil.
authors: R M
authors: RM
authors: de Araujo Soares
authors: C S
authors: CS
authors: Alviano
authors: M J
authors: MJ
authors: Esteves
authors: J
authors: J
authors: Angluster
authors: F
authors: F
authors: Costa e Silva-Filho
authors: W
authors: W
authors: de Souza
mesh_terms: D000818:Animals; D000838:Anions; D002412:Cations; D002462:Cell Membrane / Q000032:analysis / Q000502:physiology; D002855:Chromatography, Thin Layer; D003102:Colloids; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004563:Electrochemistry; D004586:Electrophoresis; D005293:Ferritins / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism; D009439:Neuraminidase / Q000494:pharmacology; D010222:Parainfluenza Virus 1, Human / Q000502:physiology; D012794:Sialic Acids / Q000032:analysis*; D014351:Trypanosomatina / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D000838:Anions; D002412:Cations; D003102:Colloids; D012794:Sialic Acids; D005293:Ferritins; D007501:Iron; D009439:Neuraminidase; D004121:Dimethyl Sulfoxide