The effects of 5-azacytidine, 12-O-tetradecanoylphorbol 13-acetate and sodium n-butyrate on reactivation of alphaherpesvirus saimiri from explant cultures of latently infected rabbit dorsal root ganglia.
The DNA hypomethylating agent 5-azacytidine greatly increased the reactivation of alphaherpesvirus saimiri-1 (alpha HVS) from latently infected rabbit dorsal root ganglia, although it inhibited the virus yield and plaque formation efficiency of alpha HVS in Vero cells. 12-O-Tetradecanoylphorbol 13-acetate (a protein kinase C activator) and sodium n-butyrate both had a stimulating action on replication in Vero cells but did not affect the release of alpha HVS from latently infected rabbit dorsal root ganglia.
Mossman
S P
SP
Department of Medical Microbiology, University of Liverpool, U.K.
Leib
D A
DA
McCarthy
K
K
Hart
C A
CA
D000818:Animals; D001374:Azacitidine / Q000494:pharmacology*; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D005727:Ganglia, Spinal / Q000382:microbiology*; D006567:Herpesvirus 2, Saimiriine / Q000187:drug effects / Q000254:growth & development* / Q000472:pathogenicity; D011817:Rabbits; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014774:Virulence; D014775:Virus Activation / Q000187:drug effects*
D002087:Butyrates; D020148:Butyric Acid; D001374:Azacitidine; D013755:Tetradecanoylphorbol Acetate