title: Purification of the serum acid-stable insulin-like growth factor binding protein from the pig (Sus scrofa).
abstract: 1. An acid-stable IGF binding protein was isolated and purified from porcine serum. 2. The protein comprised two major species with Mrs of 45 and 41 kDa determined using SDS-PAGE under reducing conditions. 3. The IGFBP preparation specifically bound both IGF-I and II. 4. Four distinct protein bands (Mrs of 23, 45, 50 and 75 kDa) in the porcine IGFBP preparation specifically bound radiolabelled IGF-I. 5. The porcine IGFBP exhibited sequence homology with IGFBPs from human plasma and rat serum. 6. This is the first report of the purification and characterization of the acid-stable IGFBP from porcine serum.
authors: Department of Dairy and Animal Science, Pennsylvania State University, University Park 16802.
authors: P E
authors: PE
authors: Walton
authors: R C
authors: RC
authors: Baxter
authors: B D
authors: BD
authors: Burleigh
authors: T D
authors: TD
authors: Etherton
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D018969:Insulin-Like Growth Factor Binding Proteins; D007700:Kinetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017451:Receptors, Somatomedin; D013002:Somatomedins / Q000097:blood*; D013552:Swine / Q000097:blood*
chemical_list: D002352:Carrier Proteins; D018969:Insulin-Like Growth Factor Binding Proteins; D017451:Receptors, Somatomedin; D013002:Somatomedins