Interferon-gamma activates the voltage-gated calcium channel in RPMI 4788 cells.
Interferon (IFN) increases cytoplasmic free calcium ([Ca2+]i) in RPMI 4788 cells, a human colon cancer cell line. Addition of IFN to the cells loaded with Fura-2, a fluorescent Ca2+ indicator, causes an immediate increase in [Ca2+]i, which is the earliest event after IFN stimulation. A cyanine dye, dis-C3- (5) was used to determine the effects of IFN on the membrane potential in cancer cells. The depolarization was seen with IFN-gamma, but not with IFN-beta. These results taken together, suggest that the IFN-gamma and -beta induced [Ca2+]i mobilization are clearly different in their dependence on Ca2+ entry through voltage-gated Ca2+ channels.
Iwagaki
H
H
First Department of Surgery, Okayama University Medical School, Japan.
Fuchimoto
S
S
Miyake
M
M
Aoki
H
H
Orita
K
K
D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D003110:Colonic Neoplasms / Q000378:metabolism; D004553:Electric Conductivity; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D007473:Ion Channels / Q000187:drug effects*; D008564:Membrane Potentials
D007473:Ion Channels; D007371:Interferon-gamma; D002118:Calcium