Ca2+-mediated action of long-chain acyl-CoA on liver mitochondria energy-linked processes.
The decrease of steady-state transmembrane potential (delta psi) and loss of accumulated Ca2+ are magnified if palmitoyl-CoA is added to rat liver mitochondria exposed to Ca2+ and phosphate. The extent of this damage increases with increasing concentration of long-chain acyl-CoA. Addition of L-carnitine with or without the addition of palmitoyl-CoA considerably delays the deenergization. In the latter case, there is a substantial decrease in the assayed endogenous long-chain acyl-CoA content. This protective action of L-carnitine is abolished by L-aminocarnitine, a powerful inhibitor of carnitine palmitoyl transferase (palmitoyl-CoA: L-carnitine O-palmitoyltransferase, EC 2.3.1.21.). The removal of Ca2+ by EGTA, or the inhibition of its uptake by Ruthenium red or Mg2+ further enhances the degree of protection.
Di Lisa
F
F
Centro Studio Fisiologia Mitocondriale, CNR, Padova, Italy.
Menabò
R
R
Miotto
G
G
Bobyleva-Guarriero
V
V
Siliprandi
N
N
D000214:Acyl Coenzyme A / Q000494:pharmacology*; D000818:Animals; D001622:Betaine / Q000031:analogs & derivatives / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology*; D002331:Carnitine / Q000494:pharmacology; D002334:Carnitine O-Palmitoyltransferase / Q000037:antagonists & inhibitors; D004533:Egtazic Acid / Q000494:pharmacology; D004734:Energy Metabolism / Q000187:drug effects*; D008274:Magnesium / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D010171:Palmitoyl Coenzyme A / Q000494:pharmacology*; D010710:Phosphates / Q000494:pharmacology; D051381:Rats; D012430:Ruthenium Red / Q000494:pharmacology
D000214:Acyl Coenzyme A; D010710:Phosphates; D012430:Ruthenium Red; D010171:Palmitoyl Coenzyme A; D001622:Betaine; D004533:Egtazic Acid; C045761:emeriamine; D002334:Carnitine O-Palmitoyltransferase; D008274:Magnesium; D002331:Carnitine; D002118:Calcium