'Perforated patch recording' allows long-term monitoring of metabolite-induced electrical activity and voltage-dependent Ca2+ currents in pancreatic islet B cells.
We describe the application of 'perforated patch recording' using the pore-forming antibiotic nystatin, to monitor the electrical activity and underlying ionic currents of rat and human pancreatic islet B cells. We demonstrate that glucose-induced electrical activity is seen even in single B cells during current-clamp recordings lasting hours 'L-type' Ca2+-channel currents can also be monitored over this period of time. This technique may prove useful in examining hormone and neurotransmitter modulation of electrical activity in B cells, while minimizing the effects of cytoplasmic 'wash-out'.
Falke
L C
LC
Department of Medicine (Jewish Hospital), Washington University, St. Louis, MO 63110.
Gillis
K D
KD
Pressel
D M
DM
Misler
S
S
D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / Q000494:pharmacology; D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001386:Azides / Q000494:pharmacology; D015220:Calcium Channels / Q000502:physiology*; D003981:Diazoxide / Q000494:pharmacology; D004553:Electric Conductivity; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D007515:Islets of Langerhans / Q000187:drug effects / Q000502:physiology*; D008564:Membrane Potentials; D009543:Nifedipine / Q000494:pharmacology; D051381:Rats; D019810:Sodium Azide; D014044:Tolbutamide / Q000494:pharmacology
D001386:Azides; D015220:Calcium Channels; D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; D019810:Sodium Azide; D014044:Tolbutamide; D009543:Nifedipine; D005947:Glucose; D003981:Diazoxide