Enzyme immunoassay of progesterone receptor in cancerous and normal endometrial tissue. A comparison with dextran coated charcoal method.
Enzyme immunoassay (EIA) was compared with the dextran-coated charcoal method and Scatchard analysis for the measurement of progesterone receptor (PgR) in cancerous and normal endometrial samples. A highly significant correlation was obtained between the two assays in both types of tissue (rs = 0.77 and 0.91, respectively; p less than 0.001). The measurable amount of PgR was usually higher with EIA (mean increase 90 and 100 per cent, respectively). EIA is suggested to be a suitable method for PgR measurement in endometrial tissue (normal and cancerous) and can also be used for analysis of PgR in the nuclear fraction.
Fernö
M
M
Department of Oncology, University Hospital, Lund, Sweden.
Borg
A
A
Horvath
G
G
Lindahl
B
B
D014408:Biomarkers, Tumor / Q000032:analysis*; D001706:Biopsy; D002467:Cell Nucleus / Q000032:analysis; D002606:Charcoal; D003600:Cytosol / Q000032:analysis; D003911:Dextrans; D004717:Endometrium / Q000032:analysis* / Q000473:pathology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008722:Methods; D011980:Receptors, Progesterone / Q000032:analysis*; D014594:Uterine Neoplasms / Q000032:analysis* / Q000473:pathology
D014408:Biomarkers, Tumor; D003911:Dextrans; D011980:Receptors, Progesterone; D002606:Charcoal