title: Histones H1(0) and H5 share common epitopes with RNA polymerase II.
abstract: We report here the cross-reaction of RNA polymerase II antiserum with histones H1(0) and H5 and the complementary cross-reactions of antisera to the globular domain of histone H1(0) (GH1(0)) and histone H5 (GH5) with RNA polymerase II. Immunoblotting of RNA polymerase II antiserum with fragments of histone H1(0) localized the cross-reaction at the junction of the globular and C-terminal domains of histone H1(0). The structural homology implied by these cross-reactions is interesting in light of reports that suggest H1(0) may play a role in differentiation and development.
authors: Department of Biological Chemistry, School of Medicine, University of California, Davis 95616.
authors: K A
authors: KA
authors: Logan
authors: M E
authors: ME
authors: Dahmus
authors: E M
authors: EM
authors: Bradbury
mesh_terms: D000818:Animals; D002417:Cattle; D002645:Chickens; D002918:Chymotrypsin; D003488:Cyanogen Bromide; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology*; D006657:Histones / Q000276:immunology*; D007106:Immune Sera / Q000276:immunology; D007118:Immunoassay; D008970:Molecular Weight; D010446:Peptide Fragments / Q000276:immunology; D012319:RNA Polymerase II / Q000276:immunology*; D012756:Sheep; D014357:Trypsin
chemical_list: D000939:Epitopes; D006657:Histones; D007106:Immune Sera; D010446:Peptide Fragments; D012319:RNA Polymerase II; D002918:Chymotrypsin; D014357:Trypsin; D003488:Cyanogen Bromide