[Two immunologically differing forms of p53 oncoprotein from B-lymphocytes of mice are coded with identical mRNA].
The detailed analysis of mRNA structure coding for p53 protein from the intact and KonA stimulated lymphocytes from mouse spleen has shown both matrices to be identical. mRNAs have been analyzed by S1 mapping. Both mRNAs directed in vitro the synthesis of p53 protein reacting with monoclonal antibodies PAb421 and PAb248 both of which are specific for one of p53 forms synthesized by lymphocytes in vivo. Thus, the phenomenon of epitopes exclusion on p53 surface from intact or activated B-lymphocytes might be explained by peculiarities of posttranslational step of protein synthesis.
Chumakov
P M
PM
Jenkins
J R
JR
Milner
R
R
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D003208:Concanavalin A / Q000494:pharmacology; D000939:Epitopes; D066298:In Vitro Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D009690:Nucleic Acid Conformation; D015513:Oncogene Proteins / Q000235:genetics / Q000276:immunology*; D010750:Phosphoproteins / Q000235:genetics / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics*; D015183:Restriction Mapping; D014158:Transcription, Genetic; D016159:Tumor Suppressor Protein p53
D000939:Epitopes; D015513:Oncogene Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D003208:Concanavalin A
Dve immunologicheski razlichaiushchiesia formy onkobelka p53 iz B-limfotsitov myshi kodiruiutsia identichnymi mRNK.