Release of nucleosomes from nuclei by bleomycin-induced DNA strand scission.
Mononucleosomes were released from both isolated mammalian (hog thyroid) and protozoan (Tetrahymena) nuclei by the bleomycin-induced DNA-strand breaking reaction. Trout sperm nuclei, on the other hand, were protected from the bleomycin-mediated DNA degradation. The mononucleosomes released from the bleomycin-treated nuclei contained the core histones H2A, H2B, H3, and H4; while HMG1 and HMG2 proteins, in addition to the core histones, were detected in the mononucleosomes obtained by micrococcal nuclease digestion of nuclei. HMGs, but not H1 histone, were dissociated into the supernatant by cleavage of chromatin DNA with bleomycin, whereas both HMGs and H1 were found in that fraction by digestion of nuclei with micrococcal nuclease. HMG1 and HMG2 were exclusively dissociated from chromatin with 1 mM bleomycin under the solvent condition where the DNA strand-breaking activity of the drug is repressed. These observations suggest the possibility that bleomycin preferentially binds to linker DNA regions not occupied by H1 histone in chromatin and exclusively dissociates HMG proteins and breaks the DNA strand. The results of the effects on bleomycin-induced DNA cleavage of nuclei of various drugs including polyamines, chelating agents, intercalating antibiotics such as mitomycin C or adriamycin, and radical scavengers are also presented.
Hamana
K
K
College of Medical Care and Technology, Gunma University, Japan.
Kawada
K
K
D000818:Animals; D001761:Bleomycin / Q000494:pharmacology*; D002467:Cell Nucleus / Q000187:drug effects*; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D003851:Deoxyribonucleases / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D007502:Iron Chelating Agents / Q000494:pharmacology; D009707:Nucleosomes / Q000187:drug effects*; D010618:Phenanthrolines / Q000494:pharmacology; D013552:Swine; D013768:Tetrahymena / Q000187:drug effects; D014337:Trout
D007502:Iron Chelating Agents; D009707:Nucleosomes; D010618:Phenanthrolines; D001761:Bleomycin; D004247:DNA; D003851:Deoxyribonucleases; C025205:1,10-phenanthroline