Variation in epitopes of the B subunit of El Tor and classical biotype Vibrio cholerae O1 cholera toxin.
Variation in epitopes of the B subunit of cholera toxin (CT-B) produced by strains of El Tor and classical biotype Vibrio cholerae O1 was examined using monoclonal antibodies prepared to V. cholerae 569B CT. CT-B epitopes were markedly conserved for V. cholerae classical biotypes. In contrast, epitope variation was observed for El Tor biotypes, which produced both a classical-like CT-B and a unique CT-B lacking at least one epitope common to 569B CT-B. The missing epitope was located outside the GM1 ganglioside-binding site. From results of the study reported here, genetic divergence is exhibited in the El Tor biotype CT-B versus classical CT-B. Furthermore, at least five unique epitopes of V. cholerae 569B CT-B can be defined.
Tamplin
M L
ML
Center of Marine Biotechnology, Maryland Biotechnology Institute, University of Maryland, Baltimore 21202.
Ahmed
M K
MK
Jalali
R
R
Colwell
R R
RR
D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001665:Binding Sites; D002772:Cholera Toxin / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D005677:G(M1) Ganglioside; D005798:Genes, Bacterial; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic / Q000378:metabolism; D014734:Vibrio cholerae / Q000145:classification / Q000235:genetics / Q000276:immunology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; C023552:choleragen receptor; D005677:G(M1) Ganglioside; D002772:Cholera Toxin