Adjuvant effect of DEAE-dextran on cholera vaccines.
Investigations into the effectiveness of DEAE-dextran as an adjuvant of whole cell cholera vaccines (Vibrio cholerae Inaba and Ogawa serotypes) were conducted using two methods: (a) the active mouse protection test, (b) the determination of antibody production in the sera of immunized mice by measuring the level of vibriocidal antibodies, and by an enzyme-linked immunosorbent assay (ELISA). In all tests, the DEAE-dextran-adjuvanted vaccine showed an antigenicity substantially superior to that achieved without adjuvants.
Joó
I
I
Human Institute for Serobacteriological Production and Research, WHO Collaborating Centre for Reference and Research on Bacterial Vaccines, Budapest, Hungary.
Emöd
J
J
D000276:Adjuvants, Immunologic; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D022121:Cholera Vaccines / Q000276:immunology*; D003637:DEAE-Dextran / Q000494:pharmacology*; D003911:Dextrans / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007159:Immunologic Tests / Q000379:methods; D008297:Male; D051379:Mice
D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D022121:Cholera Vaccines; D003911:Dextrans; D003637:DEAE-Dextran