A T-cell clone recognizing an MLC stimulating epitope located on the DRw11 beta 1 chain.
An alloreactive proliferative T-cell clone, 6065 WS, was obtained in an intrafamilial cell combination where the stimulator was a homozygous DRw11, DRw52, DQw3 son and the responder his haploidentical mother. Proliferation assays on eight local DRw11 families, 75 homozygous B-cell lines (Tenth Histocompatibility Workshop panel) and blocking assays with monoclonal antibodies showed that clone 6065 WS recognizes an epitope on the DRw11 beta 1 chain. Comparison of the reactivity of clone 6065 WS with cells expressing the three known DRw11 beta 1 amino acid sequences identified two unique amino acids at positions 71 and 86 which contribute to determining the specific recognition by the T-cell clone 6065 WS. Our data suggest that one or both of these amino acids can either be directly involved in the recognition by the T-cell receptor or responsible for critical conformation of the determinants on the DR molecule. Alternatively, they could affect recognition of a self peptide bound to the major histocompatibility complex class II molecule.
Urlacher
A
A
Laboratoire d'Histocompatibilité, Centre Régional de Transfusion Sanguine, Strasbourg, France.
Tiercy
J M
JM
Schlesier
M
M
Steimle
V
V
Peter
H
H
Jeannet
M
M
Mach
B
B
Tongio
M M
MM
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D002999:Clone Cells; D000939:Epitopes / Q000032:analysis*; D005260:Female; D006684:HLA-DR Antigens / Q000276:immunology*; D059810:HLA-DR Serological Subtypes; D006801:Humans; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D009693:Nucleic Acid Hybridization; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006684:HLA-DR Antigens; D059810:HLA-DR Serological Subtypes; C080766:HLA-DR11 antigen