A device for the production of fresh tumor slices for cytotoxic drug testing.
The potential suitability of thin slices of fresh tumor tissue for the in vitro testing of antitumor drugs was studied. With a simple device, sets of tumor slices of equal thickness were produced rapidly with minimal cellular damage. The sections showed a reproducible stable incorporation of 3H-thymidine for incubation periods of more than 24 h. Preliminary data on the effect of cytotoxic drugs on sections of xenografts of human squamous cell carcinoma revealed a distinct correlation between their in vitro effects on 3H-thymidine incorporation and their in vivo sensitivity in nude mice.
Elprana
D
D
Department of Otorhinolaryngology, University Hospital, Nijmegen, The Netherlands.
Schwachöfer
J
J
Kuijpers
W
W
D000818:Animals; D001761:Bleomycin / Q000494:pharmacology; D002945:Cisplatin / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D006801:Humans; D051379:Mice; D008867:Microtomy / Q000295:instrumentation*; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000473:pathology*
D001761:Bleomycin; D002945:Cisplatin