Cytotoxic T lymphocytes in reticuloendotheliosis virus-infected chickens.
Cytotoxic T lymphocytes were functionally demonstrated in spleen cells from chickens 7 days post inoculation with reticuloendotheliosis virus using a Cr-release assay. Major histocompatibility complex (MHC)-restricted cytotoxicity was demonstrated using effector and target cells from two different strains of chickens of known avian MHC haplotype. Anti-viral specificity was shown and in vivo generation of MHC-restricted cytotoxicity was evaluated. Cytotoxic T cells were distinguished from macrophages and natural killer cells. Their cytotoxicity was not antibody dependent. Higher levels of cytolysis were found with cytotoxic T cells from embryonally bursectomized vs. intact chickens over a large range of effector to target cell ratios. Using monoclonal antibodies, cytotoxic T cells were further defined as Ia+ T cells by immunofluorescence, antibody plus complement-mediated lysis of effector cells and blocking of cytolysis in the Cr-release assay.
Weinstock
D
D
Department of Avian and Aquatic Animal Medicine, NYSCVM, Ithaca 14853.
Schat
K A
KA
Calnek
B W
BW
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D002645:Chickens; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000276:immunology; D006649:Histocompatibility Antigens / Q000235:genetics; D007694:Killer Cells, Natural / Q000276:immunology; D008213:Lymphocyte Activation; D001356:Reticuloendotheliosis virus / Q000276:immunology*; D012190:Retroviridae / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014412:Tumor Virus Infections / Q000235:genetics / Q000276:immunology*
D000954:Antigens, Surface; D000939:Epitopes; D006649:Histocompatibility Antigens