Molecular cloning of human neutrophil elastase.
The human U937 cell line, a promonocyte-like leukemic line, has been shown to synthesize neutrophil elastase (Senior, R. M. et al. (1982) J. Clin. Invest. 69, 384-393). We have constructed a lambda gt11 cDNA library using RNA from this cell line and isolated a clone encoding part of the protein sequence of neutrophil elastase. Nucleotide sequencing indicates the clone encodes residues 108-243 (standard chymotrypsinogen numbering) of the enzyme, plus a hitherto unsuspected 20 residue-C-terminal extension of unusual structure. Comparison of the nucleotide and amino-acid sequence of elastase with the recently reported sequence of medullasin, a proteinase isolated from bone marrow, suggests they are identical.
Farley
D
D
Department of Biochemistry, University of Georgia, Athens.
Salvesen
G
G
Travis
J
J
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000201:enzymology*; D009841:Oligonucleotides / Q000096:biosynthesis; D010196:Pancreatic Elastase / Q000096:biosynthesis* / Q000235:genetics; D012313:RNA / Q000032:analysis
D004274:DNA, Recombinant; D009841:Oligonucleotides; D012313:RNA; D010196:Pancreatic Elastase