Analysis of the androgen binding in human benign prostatic hypertrophy.
In order to delineate optimal conditions for the determination of androgen specific binding in human benign prostatic hypertrophy tissues (BPH), extracts of these tissues were prepared using buffers of different compositions. The binding of 5 alpha-dihydrotestosterone (DHT) and of methyltrienolone (R 1881) was analyzed using agar gel electrophoresis. This method allowed the detection of 3 different high affinity tissular binding peaks with similar specificity. Moreover, the inhibition by each of the competitors was also the same for both ligands. It could be demonstrated that none of the observed peaks resulted from the binding of 1 of the ligands to sex hormone binding globulin (SHBG) or to a progesterone receptor. Hypotheses about the possible origin of these peaks are discussed.
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Service de Médecine et Laboratoire d'Investigation Clinique H.J. Tagnon, Institut Jules Bordet, Bruxelles, Belgium.
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D001667:Binding, Competitive; D002021:Buffers; D002606:Charcoal; D003600:Cytosol / Q000378:metabolism; D003911:Dextrans; D013196:Dihydrotestosterone / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004789:Enzyme Activation / Q000187:drug effects; D004963:Estrenes / Q000378:metabolism; D006801:Humans; D008297:Male; D015741:Metribolone; D008982:Molybdenum / Q000494:pharmacology; D011374:Progesterone / Q000378:metabolism; D011470:Prostatic Hyperplasia / Q000378:metabolism*; D011480:Protease Inhibitors / Q000494:pharmacology; D011944:Receptors, Androgen / Q000378:metabolism*; D013644:Tartrates / Q000494:pharmacology; D013696:Temperature; D045165:Testosterone Congeners / Q000378:metabolism
D002021:Buffers; D003911:Dextrans; D004963:Estrenes; D011480:Protease Inhibitors; D011944:Receptors, Androgen; D013644:Tartrates; D045165:Testosterone Congeners; D013196:Dihydrotestosterone; D002606:Charcoal; D015741:Metribolone; D011374:Progesterone; D008982:Molybdenum; C024687:sodium molybdate(VI); C029768:tartaric acid