title: [Monoclonal antibodies to an artificial immunogen, specifically demonstrating phosphotyrosine-containing proteins].
abstract: The importance of protein phosphorylation at tyrosyl hydroxy groups in the control of cell proliferation has recently been established. For identification of tyrosine-phosphorylated proteins, monoclonal antibodies (Mabs) against artificial immunogens containing O-phosphotyrosine (pTyr) or tripeptide pTyr-Gly-Gly as haptens were generated; the haptens were coupled to carrier proteins (bovine serum albumin, human immunoglobulin, keyhole limpet hemocyanin). After immunization of mice with pTyr coupled to keyhole limpet hemocyanin, Mabs were generated which were highly specific for pTyr and did not cross-react with O-phosphoserine, O-phosphothreonine, tyrosine or nucleoside-5'-monophosphates. The Mabs specifically react with tyrosinephosphorylated proteins in the Rous sarcoma virus-transformed rat XC-cell.
authors: A V
authors: AV
authors: Paniutich
authors: B I
authors: BI
authors: Rubikaĭte
authors: N N
authors: NN
authors: Voĭtenok
authors: E A
authors: EA
authors: Shidlovskaia
authors: S L
authors: SL
authors: Grokhovskiĭ
authors: A L
authors: AL
authors: Zhuze
authors: O O
authors: OO
authors: Favorova
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002461:Cell Line, Transformed; D004591:Electrophoresis, Polyacrylamide Gel; D006241:Haptens / Q000276:immunology; D007124:Immunoenzyme Techniques; D051379:Mice; D010750:Phosphoproteins / Q000276:immunology / Q000378:metabolism*; D010766:Phosphorylation; D019000:Phosphotyrosine; D011233:Precipitin Tests; D011817:Rabbits; D051381:Rats; D014443:Tyrosine / Q000276:immunology / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D006241:Haptens; D010750:Phosphoproteins; D019000:Phosphotyrosine; D014443:Tyrosine
vernacular_title: Monoklonal'nye antitela k iskusstvennomu immunogenu, spetsificheski vyiavliaiushchie fosfotirozinosoderzhashchie belki.