title: Expression and processing of human immunodeficiency virus type 1 gag and pol genes by cells infected with a recombinant vaccinia virus.
abstract: Human cells infected with a recombinant vaccinia virus containing human immunodeficiency virus type 1 gag-pol genes produced large amounts of human immunodeficiency virus gag proteins beginning at 1 h and peaking at 48 h postinfection. We show that these polyproteins are processed accurately into mature forms and that the viral polymerase gene is encoded as a 160-kilodalton gag-pol fusion protein, most likely by translational frameshifting from the gag into the pol reading frame.
authors: Department of Pathology, Stanford University School of Medicine, California 94305.
authors: S D
authors: SD
authors: Gowda
authors: B S
authors: BS
authors: Stein
authors: K S
authors: KS
authors: Steimer
authors: E G
authors: EG
authors: Engleman
mesh_terms: D002478:Cells, Cultured; D004274:DNA, Recombinant; D015683:Gene Products, gag; D006678:HIV / Q000235:genetics*; D008970:Molecular Weight; D011499:Protein Processing, Post-Translational; D012194:RNA-Directed DNA Polymerase / Q000235:genetics*; D012191:Retroviridae Proteins / Q000235:genetics*; D014616:Vaccinia virus
chemical_list: D004274:DNA, Recombinant; D015683:Gene Products, gag; D012191:Retroviridae Proteins; D012194:RNA-Directed DNA Polymerase