The distribution of MspI-induced breaks in human lymphocyte chromosomes and its relationship to common fragile sites.
The restriction endonuclease MspI (cleavage site C/CGG) induces chromosomal breaks in human lymphocytes. The breakpoints are distributed non-randomly along the chromosomes and the pattern of MspI-induced breakage depends on the recovery time (20 h or 6 h). Chromosomal bands preferentially involved in breakage are likely to coincide with bands where common fragile sites are located.
Porfirio
B
B
Department of Public Health and Cell Biology, 2nd University of Rome, Italy.
Tedeschi
B
B
Vernole
P
P
Caporossi
D
D
Nicoletti
B
B
D002869:Chromosome Aberrations; D002871:Chromosome Banding; D043283:Chromosome Fragile Sites; D002873:Chromosome Fragility; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D006801:Humans; D007621:Karyotyping; D008214:Lymphocytes / Q000648:ultrastructure*
D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific