Use of modified Fraser's stain in Promoting Activity Test (PAT).
The Promoting Activity Test (PAT) requires a staining procedure that allows rapid, accurate and reliable counting of mitotic figures. We propose use of Fraser's kernechtrot-crystal violet technique, but eliminating the picric-alcoholic differentiation to avoid fading. This modified protocol gives higher mitotic counts in adult mouse adrenal cortex than the hematoxylin-eosin originally used, especially with respect to less conspicuous prophases.
Borràs
M
M
Laboratori Fides, Barcelona, Spain.
D000302:Adrenal Cortex / Q000032:analysis / Q000166:cytology*; D000818:Animals; D005840:Gentian Violet; D051379:Mice; D008938:Mitosis; D008940:Mitotic Index; D009152:Mutagenicity Tests / Q000379:methods*; D013194:Staining and Labeling
D005840:Gentian Violet