Quantitative HPLC analysis of acidic opines by phenylthiocarbamyl derivatization.
A new method for the quantitative analysis of acidic opines--alanopine, strombine, tauropine, and beta-alanopine--is presented. The method is based on formation of phenylthiocarbamyl (PTC) derivatives of the acidic opines after partial purification by ion-exchange chromatography. The PTC acidic opines are separated by reverse-phase high-performance liquid chromatography (HPLC) and detected within 20 min by ultraviolet absorbance. This HPLC method gives higher sensitivity, 10-30 pmol minimum detection, and better reproducibility than the high-voltage paper electrophoresis method. There is also good agreement for the three acidic opines (alanopine, strombine, and tauropine) when compared by HPLC and electrophoresis methods. Accumulation of beta-alanopine was observed for the first time in the adductor muscle of blood shell, Scapharca broughtonii, during aerial exposure by application of the HPLC detection method.
Sato
M
M
School of Fisheries Sciences, Kitasato University, Iwate Prefecture, Japan.
Suzuki
S
S
Yasuda
Y
Y
Kawauchi
H
H
Kanno
N
N
Sato
Y
Y
D000409:Alanine / Q000031:analogs & derivatives* / Q000032:analysis; D000603:Amino Acids, Sulfur / Q000032:analysis*; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods; D005998:Glycine / Q000031:analogs & derivatives*; D007202:Indicators and Reagents; D017879:Isothiocyanates; D008974:Mollusca / Q000032:analysis*; D012521:Sarcosine / Q000031:analogs & derivatives* / Q000032:analysis; D013861:Thiocyanates
D000603:Amino Acids, Sulfur; D007202:Indicators and Reagents; D017879:Isothiocyanates; D013861:Thiocyanates; C005441:phenylisothiocyanate; C026051:alanopine; C050557:tauropine; C026052:strombine; D000409:Alanine; D005998:Glycine; D012521:Sarcosine