Specific endotoxic lipopolysaccharide-binding proteins on murine splenocytes. I. Detection of lipopolysaccharide-binding sites on splenocytes and splenocyte subpopulations.
Experiments have been carried out using a unique radio-iodinated, disulfide-reducible, photoactivatable LPS derivative (ASD-LPS) to detect specific LPS-binding proteins on murine splenocytes. Fractionation of LPS-photo-cross-linked, reduced, and solubilized splenocyte extracts on two-dimensional polyacrylamide gels has allowed the identification of an 80-kDa LPS-binding protein with approximate pI of 6.5. This LPS-binding protein is present on partially purified populations of splenic B lymphocytes, T lymphocytes, and macrophages. It is also the dominant LPS-binding protein on the murine 70Z/3 B cell line and the YAC-1 and EL4 T cell lines but is not detectable on the undifferentiated murine Sp2/0 myeloma cell line. Of potential importance is the fact that the 80-kDa protein appears to be indistinguishable when photolabeled extracts of splenocytes from the C3HeB/FeJ (lpsn) and LPS-nonresponder C3H/HeJ (lpsd) mice are compared.
Lei
M G
MG
Department of Microbiology, University of Kansas Medical Center, Kansas City 66103.
Morrison
D C
DC
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D002460:Cell Line; D004731:Endotoxins / Q000378:metabolism*; D005260:Female; D005802:Genes, MHC Class II; D007113:Immunity, Innate; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides / Q000378:metabolism* / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000302:isolation & purification*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H / Q000235:genetics; D008819:Mice, Nude; D008970:Molecular Weight; D011971:Receptors, Immunologic / Q000302:isolation & purification*; D013154:Spleen / Q000166:cytology*; D013601:T-Lymphocytes / Q000378:metabolism
D004731:Endotoxins; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D011971:Receptors, Immunologic; C025357:endotoxin binding proteins