Improved SDS-Page method for protein profiles analysis of actinomyces species.
A simple, rapid SDS-Page method for protein profiles analysis of Actinomyces species was developed. Eighteen strains (12 reference strains and 6 fresh isolates) representing four species were used in this study. Eight detergents were tested for protein extraction. Cell extracts were obtained by shaking the bacteria suspended in a solution containing a detergent and glass beads. After electrophoresis, according to the Laemmli's technique, two protein stain procedure were tested (Coomassie blue and silver stain). Best results were obtained with 0.5% Triton X-100 for 4 h and the silver stain procedure of Oakley. Protein profiles analysis showed that the method is reproducible and distinguishes not only species but sometimes also subspecies.
Barsotti
O
O
Laboratoire de Microbiologie-Immunologie, Faculté d'Odontologie, Lyon, France.
Comby
C
C
Morrier
J J
JJ
Benay
G
G
Decoret
D
D
Guinet
R
R
Dumont
J
J
D000190:Actinomyces / Q000032:analysis* / Q000145:classification; D001426:Bacterial Proteins / Q000032:analysis*; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D012394:Rosaniline Dyes; D012834:Silver; D012967:Sodium Dodecyl Sulfate; D013194:Staining and Labeling
D001426:Bacterial Proteins; D003902:Detergents; D012394:Rosaniline Dyes; D012967:Sodium Dodecyl Sulfate; D012834:Silver; C048139:Coomassie blue