Fatty acid binding proteins in bovine intestinal mucosa.
Cytosol obtained from bovine intestinal mucosa, contains two protein fractions that bind sulfobromophthalein and are able to remove [1-14C] palmitic acid from microsomal membranes. The high molecular weight protein fraction (F1) increases the binding of sulfobromophthalein 2 and 8 times respectively after heating at 60 degrees C during 5 min or delipidation. These changes do not correlate with the rate of palmitic acid removal from microsomes. F1 native or delipidated is more efficient than the low molecular weight protein (F2) on the removal of [1-14C]palmitic acid from microsomes. Two protein fractions DE-I and DE-II obtained from F1 by DEAE-cellulose chromatography have palmitic acid- and sulfobromophthalein-binding capacities respectively.
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Cátedra de Bioquímica, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Argentina.
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D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002417:Cattle; D003600:Cytosol / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D008970:Molecular Weight; D009363:Neoplasm Proteins; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000378:metabolism; D013448:Sulfobromophthalein / Q000378:metabolism
D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D009363:Neoplasm Proteins; D010169:Palmitic Acids; D013448:Sulfobromophthalein; D019308:Palmitic Acid