Comparison of the quantity of estrogen receptors in human endometrium and myometrium by steroid-binding assay and enzyme immunoassay based on monoclonal antibodies to human estrophilin.
The recently developed enzyme immunoassay for estrogen receptors is more simple to perform with quality assurance than conventional steroid-binding assays with radioactive labeled estrogen. However, it is not known how well the results of the two assays agree for normal human uterine samples. We compared enzyme immunoassay (Abbott estrogen receptor enzyme immunoassay) and steroid-binding assay of normal human endometrium and myometrium. Low-salt, "cytosol" estrogen receptor determinations were performed by dextran-coated charcoal assay, and high-salt, "nuclear" estrogen receptors were measured by controlled pore glass bead assay. Results showed excellent correlation (p less than 0.0001) for cytosol estrogen receptor of endometrium (r = 0.95) and myometrium (r = 0.79) and also for a smaller number of nuclear estrogen receptors of myometrium (p less than 0.01, r = 0.69). Good agreement between steroid-binding assay and enzyme immunoassay was seen for estrogen receptors of both proliferative and secretory phase samples. Thus the data indicate that the simpler estrogen receptor enzyme immunoassay is useful to measure estrogen receptor in the normal uterus. Furthermore, with this sandwich assay, there is no evidence for the existence of significant quantities of receptor fragments that do not bind estrogen.
Senekjian
E K
EK
Department of Obstetrics and Gynecology, University of Chicago, Pritzker School of Medicine, IL.
Press
M F
MF
Blough
R R
RR
Herbst
A L
AL
DeSombre
E R
ER
D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D002352:Carrier Proteins / Q000276:immunology; D002606:Charcoal; D003600:Cytosol / Q000032:analysis; D003911:Dextrans; D004717:Endometrium / Q000032:analysis*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008597:Menstrual Cycle; D009215:Myometrium / Q000032:analysis*; D011960:Receptors, Estrogen / Q000032:analysis*; D013256:Steroids
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D003911:Dextrans; D011960:Receptors, Estrogen; D013256:Steroids; C010140:estrophilin; D002606:Charcoal