In vitro transformation of rat cells by 3-methylcholanthrene: activation of the ras oncogene.
Rat cells of the established, immortalized line rat-2 were treated with the polycyclic aromatic hydrocarbon 3-methylcholanthrene. No characteristic morphological transformation occurred during three weeks after treatment. However, the carcinogen-treated cells formed colonies in soft agar. Cell lines established from single soft agar colonies were characterized by an increased proliferation rate, an enhanced colony formation in soft agar and an increased expression of the Ha-ras oncogene.
Ehm
I
I
Central Institute for Cancer Research, Academy of Sciences of the GDR, Berlin.
Denner
J
J
Jandrig
B
B
Nissen
E
E
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects*; D011905:Genes, ras; D015151:Immunoblotting; D008748:Methylcholanthrene / Q000494:pharmacology*; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000235:genetics; D051381:Rats
D008748:Methylcholanthrene; D012313:RNA