In vivo transcription of a cloned prosimian primate SINE sequence.
SINE transcription was studied by introducing a galago Monomer family member (gal39), into the mouse Ltk- cell line. Cells transiently transfected with gal39 produce gal39-specific RNA polymerase III-directed transcripts. In permanent cell lines gal39 expression was largely shut off. Genomic environment, copy number and tandem repetition of integrated SINE sequences influenced whether or not RNA polymerase III-directed gal39 transcripts were detectable. These transcripts differ in length from the observed endogenous transcript in cultured galago cells which hybridizes to this repetitive DNA family.
Slagel
V K
VK
Department of Biological Chemistry, UCLA School of Medicine 90024.
Deininger
P L
PL
D000818:Animals; D015152:Blotting, Northern; D002999:Clone Cells; D004247:DNA / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005701:Galago / Q000235:genetics*; D015870:Gene Expression; D051379:Mice; D008969:Molecular Sequence Data; D010957:Plasmids; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic; D014162:Transfection
D012313:RNA; D004247:DNA