Two monoclonal antibodies recognizing different epitopes of blood group N antigen.
Two monoclonal antibodies, N/61 (IgG2b) and N/92 (IgG2a), reacting preferentially with blood group N antigen, were obtained by immunization of BALB/c mice with human red cell membrane glycophorins. The antibodies showed distinctly different specificities. N/61 reacted with an epitope with NH2-terminal Leu residue, its free amino group, and sialic acid residues as essential components. The antibody N/92 reacted equally well with untreated and desialylated N glycoprotein, but did not react with de-O-glycosylated antigen. It seemed to recognize an epitope including both blood group N-characteristic amino acid residues, namely the 1st Leu and 5th Glu. The antibodies also differed in pH-dependence. N/61 showed maximal activity at pH 6-7 with tendency to decrease at higher pH values, whereas N/92 was not or weakly active up to pH 7, and showed a strong increase of activity at pH range 7-8.
Waśniowska
K
K
Department of Immunochemistry, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.
Duk
M
M
Steuden
I
I
Czerwiński
M
M
Wiedłocha
A
A
Lisowska
E
E
D000371:Agglutination; D000818:Animals; D000911:Antibodies, Monoclonal; D001666:Binding Sites, Antibody; D015153:Blotting, Western; D002918:Chymotrypsin / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D006021:Glycophorins / Q000276:immunology; D006863:Hydrogen-Ion Concentration; D008951:MNSs Blood-Group System / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009439:Neuraminidase / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006021:Glycophorins; D008951:MNSs Blood-Group System; D009439:Neuraminidase; D002918:Chymotrypsin; D014357:Trypsin