In vitro transcription enhancement by purified derivatives of the glucocorticoid receptor.
Mammalian glucocorticoid receptors enhance transcription from linked promoters by binding to glucocorticoid response element (GRE) DNA sequences. Understanding the mechanism of receptor action will require biochemical studies with purified components. Enhancement was observed in vitro with derivatives of the receptor that were expressed in Escherichia coli, purified, and added to a cell-free extract from Drosophila embryo nuclei. Transcription from promoters linked to one or multiple GREs was selectively enhanced by as much as six times. The effect was weaker with only one GRE, and enhancement was abolished by a point mutation that inactivates the GRE in vivo.
Freedman
L P
LP
Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0448.
Yoshinaga
S K
SK
Vanderbilt
J N
JN
Yamamoto
K R
KR
D000818:Animals; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster; D009154:Mutation; D011401:Promoter Regions, Genetic; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D013698:Templates, Genetic; D014158:Transcription, Genetic
D011965:Receptors, Glucocorticoid; D012313:RNA; D004247:DNA