title: Histone gene (H3) expression in chemically transformed oral keratinocytes.
abstract: The hamster cheek pouch is an excellent target tissue for the experimental study of oral carcinogenesis. In the course of searching for molecular alterations during the malignant transformation process, the necessity for a molecular marker for cellular proliferation became apparent. In this report, we show that the cellular level of the histone H3 mRNA is valid as a molecular index of proliferation for cycling cell populations. H3 is known to be proliferation dependent for its expression in cultured animal cells. This study shows that H3 retains its cell-cycle-dependent expression in chemically transformed oral keratinocytes. The onset of H3 mRNA synthesis couples to the onset of DNA synthesis (S-phase). The cellular level of H3 mRNA therefore is proportional to the fraction of cells in the S-phase of the cell cycle. This conveniently allows us to correlate, in asynchronized cell populations, the expression of cellular genes to their proliferation rates. We demonstrate the usefulness of this proliferation marker by presenting data that different chemically induced oral carcinomas, but not normal cheek pouch tissues, contain readily detectable levels of c-Ki-ras proto-oncogene mRNA. Probing the same RNA blot to quantitate H3 mRNA levels allowed us to conclude that the high levels of c-Ki-ras mRNA in tumor tissues was likely due to the increased growth rate of the tumor tissues and not due to the deregulated expression of this cellular-proto-oncogene.
authors: Department of Oral Medicine and Oral Pathology, Harvard School of Dental Medicine, Boston, Massachusetts 02115.
authors: D T
authors: DT
authors: Wong
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002453:Cell Cycle; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D006224:Cricetinae; D004817:Epidermis; D005786:Gene Expression Regulation; D006657:Histones / Q000235:genetics*; D007399:Interphase; D007633:Keratins; D009062:Mouth Neoplasms / Q000235:genetics*; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000096:biosynthesis*; D012334:RNA, Neoplasm / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
chemical_list: D006657:Histones; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D007633:Keratins