An enzyme immunoassay for the measurement of HLA-DR antigen expression.
A simple, sensitive, rapid and reproducible method is described for the measurement of HLA-DR expression on monocytes. The assay is based on an enzyme-linked immunostaining of the cells in suspension form followed by colour development. The non-specific binding of anti-HLA-DR to Fc receptors was taken into account by incubation of cells with an irrelevant monoclonal antibody (antirubella-G7H9). The binding of the anti-rubella monoclonal with the cells was subtracted from the binding of anti-HLA-DR to cells. Using three different cell lines (K562, CTLL and Daudi), the specificity of the assay proved to be acceptable and the assay remained sensitive even when only 10(4) cells/well were used. The assay has a number of potential applications.
Nouri-Aria
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KT
Liver Unit, King's College School of Medicine and Dentistry, London, U.K.
Williams
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Eddleston
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D000818:Animals; D002460:Cell Line; D000939:Epitopes / Q000032:analysis; D006681:HLA-D Antigens / Q000032:analysis*; D006684:HLA-DR Antigens / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D007166:Immunosuppressive Agents / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D009000:Monocytes / Q000032:analysis
D000939:Epitopes; D006681:HLA-D Antigens; D006684:HLA-DR Antigens; D007166:Immunosuppressive Agents; D008070:Lipopolysaccharides; D007371:Interferon-gamma