[Substrate properties of C'-methylnucleoside triphosphates in a reaction of RNA synthesis catalyzed by Escherichia coli RNA-polymerase].
2 theta-C-methyl substituted and phosphonate analogs of UTP were prepared and together with the synthesized earlier 3'-C-methyl-UTP were investigated in the RNA synthesis reaction catalysed by Escherichia coli RNA-polymerase. Substrate properties of UTP analogs were studied in the presence of all natural triphosphates, in the absence of UTP and under conditions of soil substrate reaction. It was shown that UTP(3'CH3) is incorporated into the RNA chain and terminates further RNA elongation. Another analog UTP (2'CH3) may substitute natural UTP in RNA synthesis, but the effectivity of its incorporation is diminished. Phosphonate analog UTP(5'CH2) is a pseudoterminator of RNA synthesis. The conformational analysis of 2'- and 3'C-methylnucleosides by force-field method of calculation was carried out in order to find energetically forbidden conformations of these analogs due to the collision of bulky methyl group and a heterocyclic base. An attempt was made to fix the conformation of the substrate during its enzymatic transformation.
Savochkina
L P
LP
Sviriaeva
T V
TV
Beĭgel'man
L N
LN
Padiukova
N Sh
NSh
Kuznetsov
D A
DA
Lysov
Iu P
IuP
Mikhaĭlov
S N
SN
Bibilashvili
R Sh
RSh
D001483:Base Sequence; D002384:Catalysis; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D008969:Molecular Sequence Data; D012313:RNA / Q000096:biosynthesis*; D013379:Substrate Specificity; D014544:Uridine Triphosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000378:metabolism
D012313:RNA; D012321:DNA-Directed RNA Polymerases; D014544:Uridine Triphosphate
Substratnye svoĭstva C'-metilnukleozidtrifosfatov v reaktsii sinteza RNK, kataliziruemoĭ RNK-polimerazoĭ Escherichia coli.