Application of Neuhoff's optimized Coomassie brilliant blue G-250/ammonium sulfate/phosphoric acid protein staining to ultrathin polyacrylamide gels on polyester films.
An optimized Coomassie staining procedure, utilizing Coomassie Brilliant Blue G-250 in phosphoric acid/ammonium sulfate, was applied to ultrathin-layer isoelectric focusing in 0.18 mm polyacrylamide gels, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis in 0.38 mm polyacrylamide gels, both backed to Gel-Fix polyester supporting films. After isoelectric focusing staining of gelatin and acidic proteins was better with the phosphoric acid/ammonium sulfate procedure than with conventional organic solvent methods. When applied to gels after sodium dodecyl sulfate-polyacrylamide gel electrophoresis the sensitivity of the phosphoric acid/ammonium sulfate method was equal to that on conventional staining but lower than on silver staining.
Peisker
K
K
St.-Elisabeth-Hospital, Halle, German Democratic Republic.
D000180:Acrylic Resins; D000645:Ammonium Sulfate; D004591:Electrophoresis, Polyacrylamide Gel; D005782:Gels; D007525:Isoelectric Focusing; D010756:Phosphoric Acids; D011091:Polyesters; D011506:Proteins / Q000032:analysis*; D012394:Rosaniline Dyes; D013194:Staining and Labeling / Q000379:methods*
D000180:Acrylic Resins; D005782:Gels; D010756:Phosphoric Acids; D011091:Polyesters; D011506:Proteins; D012394:Rosaniline Dyes; C016680:polyacrylamide gels; C004692:coomassie Brilliant Blue; D000645:Ammonium Sulfate