Treatment of human clonogenic tumor cells and bone marrow progenitor cells with bleomycin and peplomycin under 40.5 degrees C hyperthermia in vitro.
Tumor cells derived from 13 different individual human tumors were plated in a colony forming monolayer assay. The effect of bleomycin and peplomycin on colony formation was assessed in normothermic conditions and after a hyperthermic treatment at 40.5 degrees C for 2 h at the beginning of the culture. In three out of the 13 tumor samples (two colon carcinomas, one malignant melanoma), hyperthermic incubation resulted in a thermal enhancement of the effects of bleomycin and peplomycin. In addition, human bone marrow progenitor cells (CFU-C) were subjected to the same procedure. Peplomycin proved to be less toxic to CFU-C than bleomycin. In samples from eight different donors, homogeneous dose-response curves were observed. There was no difference between normo- and hyperthermic incubation.
Neumann
H A
HA
Medizinische Klinik der Ruhr Universität, St Josef Hospital, Bochum, F.R.G.
Herrmann
D B
DB
Fiebig
H H
HH
Engelhardt
R
R
D001761:Bleomycin / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms / Q000628:therapy; D003114:Colony-Forming Units Assay; D004305:Dose-Response Relationship, Drug; D005706:Gallbladder Neoplasms / Q000628:therapy; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006358:Hot Temperature; D006801:Humans; D008175:Lung Neoplasms / Q000628:therapy; D008545:Melanoma / Q000628:therapy; D009217:Myosarcoma / Q000628:therapy; D014411:Neoplastic Stem Cells / Q000187:drug effects*; D017663:Peplomycin; D014410:Tumor Stem Cell Assay
D001761:Bleomycin; D017663:Peplomycin