Plasmodium falciparum: analysis of B epitopes of a polypeptide antigen expressed in Escherichia coli, using monoclonal antibodies.
Monoclonal antibodies were raised against a recombinant molecule corresponding to the polypeptide 72 kDa, previously described as possibly related to protection in Plasmodium falciparum infection. Selection of hybridoma cell lines was done by immunofluorescence to guarantee the reactivity of the monoclonal antibodies both against the recombinant and the native molecule of the parasite. Monoclonal antibodies were characterized by serological and immunochemical techniques. Competitive binding assays between monoclonal antibodies defined four different B epitopes. One epitope is specific for P. falciparum, a second is also present in P. vivax, while the two others seem to be ubiquitous and are also present in the rodent parasite P. chabaudi. The ubiquitous epitope 72.C is apparently the only one recognized by squirrel monkey sera presenting protective antibodies against the asexual blood infection by P. falciparum.
Blisnick
T
T
Department of Immunology, Institut Pasteur, Paris, France.
Lema
F
F
Mazie
J C
JC
Pereira da Silva
L P
LP
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000953:Antigens, Protozoan / Q000032:analysis*; D001667:Binding, Competitive; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D004926:Escherichia coli; D005455:Fluorescent Antibody Technique; D006825:Hybridomas; D007106:Immune Sera / Q000276:immunology; D010455:Peptides / Q000032:analysis / Q000276:immunology*; D010963:Plasmodium falciparum / Q000276:immunology*; D011233:Precipitin Tests; D011863:Radioimmunoassay; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology*; D012453:Saimiri; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D000939:Epitopes; D007106:Immune Sera; D010455:Peptides; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins