The human alpha-1-antitrypsin-related sequence gene: isolation and investigation of its expression.
A genomic sequence approximately 5 kb in length designated the alpha-1-antitrypsin-related gene (ATR), showing considerable homology to the human alpha-1-antitrypsin (AAT) gene, was identified 10 kb downstream of the authentic AAT gene. The AAT and ATR genes were introduced separately into L-cells by transfection in order to establish a method for distinguishing between expression of the two genes. RNA probes from the cloned ATR region were then used in a ribonuclease protection assay against RNA from a range of human adult and fetal tissues. No evidence of expression of ATR was found, indicating that this region is probably a pseudogene.
Kelsey
G D
GD
MRC Human Biochemical Genetics Unit, London.
Parkar
M
M
Povey
S
S
D000818:Animals; D005091:Exons; D005796:Genes; D005814:Genes, Viral; D006801:Humans; D007739:L Cells; D051379:Mice; D012313:RNA / Q000032:analysis; D015347:RNA Probes; D000515:alpha 1-Antitrypsin / Q000235:genetics* / Q000378:metabolism
D015347:RNA Probes; D000515:alpha 1-Antitrypsin; D012313:RNA