Separation of two fractions from rabbit anti-BPTI antibody by affinity chromatography.
Non-competitive enzyme-linked immunosorbent assay (ELISA) for rabbit anti-BPTI antibody is described. The antibody was purified by affinity chromatography on Sepharose-BPTI column and then separated into two antibody fractions on Sepharose-trypsin (BPTI) column. The fraction I antibody, not retarded on the second biosorbent column, efficiently inactivated BPTI, but the fraction II antibody, eluted from the column with glycine buffer, pH 2.2 was firmly bound to active BPTI. This proves that BPTI has at least two epitopes.
Kurowska
E
E
Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.
Szewczuk
A
A
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D007611:Aprotinin / Q000276:immunology*; D002417:Cattle; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D008168:Lung / Q000201:enzymology; D010179:Pancreas / Q000201:enzymology; D011817:Rabbits
D000906:Antibodies; D000939:Epitopes; D007611:Aprotinin