Western blots from sodium dodecyl sulfate-polyacrylamide gels stained by metal salts.
Proteins separated by sodium dodecyl sulfate-gel electrophoresis can be stained in 5 min with zinc or copper chloride. We here report that these stained but unfixed gels can be electrophoretically transferred to nitrocellulose filters and probed immunologically with the same efficiency and sensitivity as unstained gels. In this way, an immunologically defined polypeptide can be identified with a specific stained protein band on a single gel.
Wang
D
D
Department of Biochemistry, Wayne State Medical School, Detroit, Michigan 48201.
Dzandu
J K
JK
Hussain
M
M
Johnson
R M
RM
D000180:Acrylic Resins; D015153:Blotting, Western / Q000379:methods*; D003300:Copper; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D008670:Metals; D012492:Salts; D012967:Sodium Dodecyl Sulfate; D013194:Staining and Labeling; D013205:Staphylococcal Protein A / Q000032:analysis; D015032:Zinc
D000180:Acrylic Resins; D008670:Metals; D012492:Salts; D013205:Staphylococcal Protein A; C016680:polyacrylamide gels; D012967:Sodium Dodecyl Sulfate; D003300:Copper; D015032:Zinc