The preferential accumulation of helper-inducer T lymphocytes in inflammatory lesions: evidence for regulation by selective endothelial and homotypic adhesion.
The mechanisms which lead to the accumulation of T lymphocytes into inflammatory lesions are not clearly understood. We have previously shown that synovial CD4 T lymphocytes are mostly CDw29+UCHL1+ (helper-inducer cells) and very few carry the CD45R antigen which identifies the suppressor-inducer subset. Synovial CD8+ cells are also CDw29+UCHL1+CD45R-. In the present study, lymphocytes from pleural and peritoneal inflammatory infiltrates were shown to have a similar phenotypic pattern. Furthermore, it was demonstrated that the CDw29+UCHL1+ subset had a greater ability than CD45R+ cells to adhere to endothelial cells and to form homotypic clusters. Differential surface expression of LFA-1 on the two subsets was also shown, but this could not account for the demonstrated adhesion differences. Differences in adhesion between CDw29+/UCHL1+ and CD45R+ cells may explain the preferential accumulation of CDw29+/UCHL1+ cells in inflammatory infiltrates and underlie some of the functional differences between cells taken from sites of chronic inflammation and those from peripheral blood.
Pitzalis
C
C
Division of Medicine, United Medical School, Guy's Hospital, London, GB.
Kingsley
G
G
Haskard
D
D
Panayi
G
G
D000943:Antigens, Differentiation / Q000032:analysis*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000502:physiology*; D002448:Cell Adhesion; D002465:Cell Movement; D002469:Cell Separation / Q000379:methods; D004727:Endothelium / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D007249:Inflammation / Q000503:physiopathology*; D019012:Integrin beta1; D017493:Leukocyte Common Antigens; D016169:Lymphocyte Function-Associated Antigen-1; D013582:Synovial Fluid / Q000473:pathology; D006377:T-Lymphocytes, Helper-Inducer / Q000145:classification / Q000166:cytology / Q000502:physiology*
D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D019012:Integrin beta1; D016169:Lymphocyte Function-Associated Antigen-1; D017493:Leukocyte Common Antigens