Trypanosoma brucei sspp.: cleavage of variant specific and common glycoproteins during exposure of live cells to trypsin.
Intact bloodstream forms of Trypanosoma brucei brucei, T.b. gambiense, and T.b. rhodesiense and procyclic forms of T.b. brucei and T.b. gambiense were incubated in trypsin, solubilized for gel electrophoresis, and analyzed for removal of surface molecules. Silver-stained gels and transfer blots probed with horseradish peroxidase-conjugated or radiolabeled lectins revealed that only three glycoproteins, Gp120p, Gp91p, and Gp23p, were removed from the surface of procyclic forms by trypsin. The variant specific glycoproteins, Gp23b, Gp120b, and in some clones Gp91b were surface molecules cleaved from bloodstream forms. Greater than 90% of the variant specific glycoprotein (VSG) was removed from the surface of all clones studied within 1 hr following the addition of trypsin. The removal of VSG was coincident with appearance of 37 to 50 kDa glycopeptide fragments of VSG with different clones yielding different sized fragments. Detailed kinetic analysis of proteins from whole cell extracts and supernatants of the DuTat 1.1 clone of T.b. rhodesiense using concanavalin A (Con A) and polyclonal antibodies revealed that three major VSG fragments were released during trypsinization. The electrophoretic mobility of the three VSG fragments of DuTat 1.1 was not altered when samples were boiled in sodium dodecyl sulfate to inhibit the endogenous phospholipase C. Antiserum to the cross-reactive determinant bound to intact VSG, but did not bind VSG fragments. Thus, the major Con A binding fragments of DuTat 1.1 VSG and perhaps those of the other clones we studied were probably derived from the N-terminal domain of the molecule. The data suggest that VSG is cleaved by trypsin in situ at the hinge region, but remains attached to the cell surface via weak interaction with neighboring molecules.
Frommel
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Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina 27710.
Seyfang
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A
Balber
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D003208:Concanavalin A / Q000378:metabolism; D003429:Cross Reactions; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology / Q000378:metabolism*; D007106:Immune Sera / Q000276:immunology; D007118:Immunoassay; D008565:Membrane Proteins / Q000378:metabolism; D014346:Trypanosoma brucei brucei / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D014347:Trypanosoma brucei gambiense / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D014357:Trypsin / Q000494:pharmacology*; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006023:Glycoproteins; D007106:Immune Sera; D008565:Membrane Proteins; D014643:Variant Surface Glycoproteins, Trypanosoma; D003208:Concanavalin A; D014357:Trypsin