[Development of test systems of immunoenzyme analysis for the rapid diagnosis of rotavirus infection].
A test system of enzyme immunoassay (EIA) consisting of simian rotavirus SA-11 and rabbit antiserum has been developed for the detection of rotavirus antigen. Direct EIA was used for tests on stool specimens from 289 children varying in ages from 10 days to 12 years suffering from acute enteric infections and 56 normal children. The antigen was detected in 22.1% and 3.5%, respectively, in patients predominantly in the first 3 days of the disease and most frequently in cases running as gastroenteritis. The results are discussed with reference to the possibility of the development of rotavirus carrier state and mixed virus-bacteria infections.
Bukrinskaia
A G
AG
Timina
V P
VP
Kitsak
V Ia
VIa
Pavlova
L A
LA
Moĭsiadi
S A
SA
D000208:Acute Disease; D000914:Antibodies, Viral / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D003378:Counterimmunoelectrophoresis; D000939:Epitopes / Q000032:analysis; D005243:Feces / Q000382:microbiology; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D007136:Immunoglobulins / Q000032:analysis; D007223:Infant; D007231:Infant, Newborn; D007410:Intestinal Diseases / Q000175:diagnosis; D012401:Rotavirus / Q000276:immunology*; D012400:Rotavirus Infections / Q000175:diagnosis*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D007136:Immunoglobulins
Razrabotka test-sistem immunofermentnogo analiza dlia bystroĭ diagnostiki rotavirusnoĭ infektsii.