[Identification of human semen stains using anti-p30 serum].
The present communication describes the identification of human semen stains using immunodiffusion test with anti-p30 serum. In order to evaluate objectively the value of the immunodiffusion test for the identification. Random sampling and blind study were used. The results indicated that the immunodiffusion test was more accurate than sperm detection. It was proved that p30 was a useful human semen marker especially in semen stains of azoospermia and vaseligation. The vaginal secretion and saliva in mixed stains did not interfere with the result of the immunodiffusion. The human semen stains can be differentiated from the animal semen stains with anti-p30 serum. Therefore, it is concluded that the demonstration of p30 in a suspected stain is the best method for human semen identification up to now.
Hou
Y P
YP
Wu
M Y
MY
D004311:Double-Blind Method; D006801:Humans; D007106:Immune Sera / Q000032:analysis*; D005779:Immunodiffusion; D008297:Male; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000276:immunology*; D011902:Rape / Q000331:legislation & jurisprudence; D012661:Semen / Q000032:analysis*; D029589:Seminal Plasma Proteins; D013094:Spermatozoa / Q000032:analysis; D013194:Staining and Labeling
D007106:Immune Sera; D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein