title: Dopamine actions in vitro on enzyme and electrolyte secretion from normal and sympathectomized rat parotid glands.
abstract: 1. Adult rats were denervated unilaterally by removal of the left superior cervical ganglion or chemically denervated with 6-hydroxydopamine or reserpine. Two weeks later the parotid glands were used for in vitro secretory studies and their catecholamines and major metabolites were measured. 2. Noradrenaline concentrations were reduced 2 weeks after surgical sympathectomy and reserpine pre-treatment 18 h previously, whereas 6-hydroxydopamine pre-treatment for 3 days reduced both noradrenaline and dopamine concentrations. 3. Dopamine caused a prominent amylase release from incubated control glands. However, a subsensitivity for dopamine-induced amylase release was recorded on the denervated side. 4. Dopamine caused a prominent potassium efflux measured as 86Rb+ efflux from control glands, but was without effect in denervated glands. This is in contrast to noradrenaline-induced 86Rb+ efflux which was equally effective in both denervated and control glands. 5. Dopamine caused [3H]noradrenaline efflux in control glands, but was without effect in surgically denervated glands and in glands pre-treated with reserpine or 6-hydroxydopamine. 6. It is concluded that dopamine-induced potassium release is caused by a presynaptic action on noradrenergic nerves, whereas dopamine-induced amylase release has a presynaptic and a postsynaptic component. The results suggest a specific action of dopamine in salivary glands, with different effects on enzyme release and ionic fluxes.
authors: Department of Histology and Cell Biology, University of Umeå, Sweden.
authors: A
authors: A
authors: Danielsson
authors: R
authors: R
authors: Henriksson
authors: S
authors: S
authors: Sundström
authors: P
authors: P
authors: Wester
mesh_terms: D000681:Amylases / Q000378:metabolism; D000818:Animals; D004298:Dopamine / Q000494:pharmacology*; D005260:Female; D006719:Homovanillic Acid / Q000378:metabolism; D006892:Hydroxydopamines / Q000494:pharmacology; D066298:In Vitro Techniques; D008734:Methoxyhydroxyphenylglycol / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism; D010306:Parotid Gland / Q000187:drug effects / Q000294:innervation / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D012110:Reserpine / Q000494:pharmacology; D013562:Sympathectomy
chemical_list: D006892:Hydroxydopamines; D008734:Methoxyhydroxyphenylglycol; D012110:Reserpine; D000681:Amylases; D011188:Potassium; D004298:Dopamine; D009638:Norepinephrine; D006719:Homovanillic Acid