The detection of natural opal suppressor seryl-tRNA in Escherichia coli by the dot blot hybridization and phosphorylation by a tRNA kinase [corrected] .
It was believed that there was no natural suppressor tRNA in Escherichia coli, however, it has been suggested that selC, relating to the synthesis of formate dehydrogenase of a selenoprotein [(1988) Nature 331, 723-725], codes for tRNA, even though the presence of tRNA has not yet been demonstrated. We detected the product of selC in the tRNA preparation of the E. coli MC 4100 strain by the dot blot hybridization method with a DNA probe (ACCGCTGGCGGC) corresponding to the extra arm of selC tRNA. Two hybridization peaks were found in the chromatographic pattern from Sephadex A50. The amount of tRNA was estimated to be about 0.03% of the total tRNA. Some tRNA [corrected] was phosphorylated by a tRNA kinase in E. coli B. These results suggest that the opal suppressor seryl-tRNA in E. coli should be converted to selenocysteyl-tRNA [corrected] and occurs in vertebrates as a general phenomenon.
Mizutani
T
T
Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Maruyama
N
N
Hitaka
T
T
Sukenaga
Y
Y
D002845:Chromatography; D015342:DNA Probes; D004926:Escherichia coli / Q000235:genetics*; D005560:Formate Dehydrogenases / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010766:Phosphorylation; D010770:Phosphotransferases / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor); D012329:RNA, Bacterial / Q000032:analysis* / Q000378:metabolism; D012346:RNA, Transfer, Amino Acyl / Q000032:analysis* / Q000378:metabolism
D015342:DNA Probes; D012329:RNA, Bacterial; D012346:RNA, Transfer, Amino Acyl; D005560:Formate Dehydrogenases; D010770:Phosphotransferases; D017853:Phosphotransferases (Alcohol Group Acceptor); C041177:seryl-tRNA-ATP phosphotransferase