Ovalbumin-specific human B-cell activation and maturation. The absence of final maturation is due to the incapacity of ovalbumin-activated T cells to produce maturation factors.
By means of a panel of monoclonal antibodies it is demonstrated that, in cultures of human peripheral blood mononuclear cells (PBMC) with the T-cell-dependent (TD) antigen ovalbumin (OA), responding B cells are activated from the resting state. The differentiation of the activated B cells to high rate-secreting plasma blasts, however, is arrested in an early activation phase, in which they can be detected as low rate-secreting plaque-forming cells. The arrest does not occur when stimulation with OA occurs in the presence of antigen-nonspecific activation and maturation factors, which are provided in the culture by the anamnestic response to the TD antigen tetanus toxoid.
Hart
L A
LA
Department of Immunology, University Hospital for Children and Youth, Utrecht, The Netherlands.
Zijlstra
J
J
Heijnen
J J
JJ
Ballieux
R E
RE
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D001402:B-Lymphocytes / Q000145:classification / Q000166:cytology / Q000276:immunology*; D002454:Cell Differentiation; D000939:Epitopes / Q000276:immunology*; D006462:Hemolytic Plaque Technique; D006801:Humans; D015847:Interleukin-4; D007378:Interleukins / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D010047:Ovalbumin / Q000276:immunology* / Q000494:pharmacology; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D013745:Tetanus Toxoid / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D000939:Epitopes; D007378:Interleukins; D013745:Tetanus Toxoid; D015847:Interleukin-4; D010047:Ovalbumin