Identification of a histamine release inhibitory factor produced by human mononuclear cells in vitro.
Human mononuclear cells (MNC) secrete histamine-releasing factor(s) (HRF) when cultured in vitro. HRF induces the release of histamine and other mediators from basophils and mast cells. We have shown that MNC also produced a histamine release inhibitory factor (HRIF), and that the synthesis is augmented by culture with physiologic concentrations of histamine (10(-10) to 10(-6) M) and by the mitogen concanavalin A (Con A). HRIF does not affect release initiated by other secretagogues such as allergen, anti-IgE, C5a, Con A, and phorbol myristate acetate. HRIF requires a preincubation with the cells for 5-10 min for maximal inhibition, and this effect is not abolished by washing the cells after the preincubation. The biological activity of HRIF is protease-sensitive, neuraminidase-resistant, and relatively heat-stable. HRIF can be distinguished from HRF by a lower apparent molecular mass (8,000-10,000 D).
Alam
R
R
Department of Internal Medicine, University of Texas Medical Branch, Galveston 77550.
Grant
J A
JA
Lett-Brown
M A
MA
D014408:Biomarkers, Tumor; D002478:Cells, Cultured; D003182:Complement C5 / Q000494:pharmacology; D015936:Complement C5a; D003208:Concanavalin A / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006636:Histamine Release; D006358:Hot Temperature; D006801:Humans; D008222:Lymphokines / Q000494:pharmacology*; D008970:Molecular Weight; D009439:Neuraminidase / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D000090324:Tumor Protein, Translationally-Controlled 1
D014408:Biomarkers, Tumor; D003182:Complement C5; D008222:Lymphokines; D000090324:Tumor Protein, Translationally-Controlled 1; D003208:Concanavalin A; D015936:Complement C5a; D009439:Neuraminidase; D013755:Tetradecanoylphorbol Acetate