Total protein determined in human breast milk by use of coomassie brilliant blue and centrifugal analysis.
This simple method of centrifugal analysis for total protein in human breast milk is based on the change in the wavelength of the absorbance maximum of Coomassie Brilliant Blue G-250 when the dye is bound to protein. Within-run and between-day CVs were 3.8% and 4.8%, respectively. Compared with a micro-Kjeldahl method for determination of total nitrogen, the coefficient of correlation was 0.99.
Bergqvist
Y
Y
Department of Clinical Chemistry, Falun Central Hospital, Sweden.
Karlsson
L
L
Fohlin
L
L
D000328:Adult; D001322:Autoanalysis; D002498:Centrifugation / Q000379:methods; D005260:Female; D006801:Humans; D008894:Milk Proteins / Q000032:analysis* / Q000592:standards; D008895:Milk, Human / Q000032:analysis*; D009584:Nitrogen / Q000032:analysis; D012394:Rosaniline Dyes; D013194:Staining and Labeling
D008894:Milk Proteins; D012394:Rosaniline Dyes; C004692:coomassie Brilliant Blue; D009584:Nitrogen