Influence of B-cell impairment on pancreatic acini in NOD mice and streptozotocin-induced diabetic rats.
Exocrine pancreatic function insufficiency, even of short duration, has been reported in juvenile-onset insulin dependent diabetic patients. To evaluate the status of pancreatic acini under decreased B-cell function, tissue insulin, amylase, chymotrypsinogen and trypsinogen in the pancreas were measured in streptozotocin-induced diabetic rats and non-obese diabetic mice in various conditions. In streptozotocin diabetic rats, a dissociation of three enzyme contents was demonstrated in the condition with discontinuation of insulin injection, i.e., a marked decrease in amylase, a significant increase in chymotrypsinogen, but no significant change in trypsinogen. This dissociation was markedly improved in the insulin-treated condition. In non-obese diabetic mice, these enzyme contents were not significantly changed although severe insulitis together with the marked decrease in insulin content was observed. These data show that the cessation of B-cell function alone does not cause insufficiency of exocrine pancreas.
Yokoyama
J
J
Third Department of Internal Medicine, Jikei University, School of Medicine, Tokyo, Japan.
Mori
Y
Y
Ikeda
Y
Y
Nishimura
M
M
Mullen
Y
Y
D000681:Amylases / Q000032:analysis; D000818:Animals; D002919:Chymotrypsinogen / Q000032:analysis; D003920:Diabetes Mellitus / Q000503:physiopathology*; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D005260:Female; D007328:Insulin / Q000032:analysis; D007515:Islets of Langerhans / Q000503:physiopathology*; D051379:Mice; D008815:Mice, Inbred Strains; D009765:Obesity; D010179:Pancreas / Q000032:analysis / Q000201:enzymology / Q000378:metabolism*; D051381:Rats; D011917:Rats, Inbred Lew; D014362:Trypsinogen / Q000032:analysis
D007328:Insulin; D014362:Trypsinogen; D002919:Chymotrypsinogen; D000681:Amylases