Structural differences found in the sugar chains of eutopic and ectopic free alpha-subunits of human glycoprotein hormone.
Free alpha-subunits of human glycoprotein hormone were purified from the urine of a healthy pregnant woman and from that of a patient with adenocarcinoma. Comparative study of their sugar moieties revealed that they have different numbers and different sets of asparagine-linked sugar chains, which are also different from those of alpha-subunit obtained by dissociation of whole hCG molecule. The eutopic free alpha-subunit contained biantennary complex-type sugar chains only. In contrast, the ectopic free alpha-subunit contained tri- and tetraantennary complex-type sugar chains in addition.
Kawano
T
T
Department of Biochemistry, University of Tokyo, Japan.
Endo
T
T
Nishimura
R
R
Mizuochi
T
T
Mochizuki
M
M
Kochibe
N
N
Kobata
A
A
D000230:Adenocarcinoma / Q000652:urine*; D001216:Asparagine / Q000032:analysis; D002236:Carbohydrate Conformation; D055598:Chemical Phenomena; D002621:Chemistry; D006063:Chorionic Gonadotropin / Q000302:isolation & purification; D002846:Chromatography, Affinity; D004590:Electrophoresis, Paper; D005260:Female; D015292:Glycoprotein Hormones, alpha Subunit / Q000032:analysis*; D006026:Glycoside Hydrolases; D006801:Humans; D008745:Methylation; D008956:Models, Chemical; D009439:Neuraminidase; D009844:Oligosaccharides / Q000302:isolation & purification*; D011247:Pregnancy / Q000652:urine*; D013329:Structure-Activity Relationship
D006063:Chorionic Gonadotropin; D015292:Glycoprotein Hormones, alpha Subunit; D009844:Oligosaccharides; D001216:Asparagine; D006026:Glycoside Hydrolases; D009439:Neuraminidase