Prodynorphin gene expression in spinal cord is enhanced after traumatic injury in the rat.
Levels of mRNAs coding for prodynorphin (Pro-Dyn) and proenkephalin (Pro-Enk) as well as the levels of immunoreactive (ir)-dynorphin (Dyn) and (ir)-Met-enkephalin (Met-Enk) were measured in the spinal cord of rats, 65 h following transection or injury of the spinal cord at the T6 spinal segment. Levels of both Pro-Dyn mRNA and of ir-Dyn were significantly increased between 60 and 150%, above control levels in the whole spinal cord, whereas those of Pro-Enk mRNA and of ir-Met-Enk remained unchanged. The increase in spinal levels of Pro-Dyn mRNA were highest in the areas close to the side of transection and indicate an involvement of the Pro-Dyn opioid system in the response to spinal injury and transection.
Przewłocki
R
R
Department of Neuropharmacology, Max-Planck-Institut für Psychiatrie, Planegg-Martinsried, F.R.G.
Haarmann
I
I
Nikolarakis
K
K
Herz
A
A
Höllt
V
V
D000818:Animals; D004399:Dynorphins / Q000378:metabolism; D004744:Enkephalin, Methionine / Q000378:metabolism; D004745:Enkephalins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D008297:Male; D009693:Nucleic Acid Hybridization; D011498:Protein Precursors / Q000235:genetics* / Q000378:metabolism; D012313:RNA; D018075:RNA, Complementary; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013119:Spinal Cord Injuries / Q000378:metabolism*
D004745:Enkephalins; D011498:Protein Precursors; D018075:RNA, Complementary; D012333:RNA, Messenger; C029992:proenkephalin; D004744:Enkephalin, Methionine; D012313:RNA; D004399:Dynorphins; C033660:preproenkephalin