Tissue- and species-specific expression of inhibitory guanine nucleotide-binding proteins. Cloning of a full-length complementary DNA from canine heart.
Three different isoforms of the inhibitory guanine nucleotide-binding protein alpha-subunit (Gi alpha) have been cloned. However, little information on the cardiac-specific expression of these isoforms is available. The deduced amino acid sequence of a full-length Gi alpha 2 complementary DNA from a canine ventricular library displays several unique residues compared with those from other species. In contrast to previously described complementary DNA clones for Gi alpha 2, this complementary DNA has a complete 3'-untranslated region with the expected consensus sequence for polyadenylation. Gi alpha 2 is the predominant isoform in the heart, and its message level of 14.7 +/- 4.9 pg/10 micrograms total ventricular RNA is about fourfold lower than that of the stimulatory guanine nucleotide-binding protein.
Holmer
S R
SR
Cellular and Molecular Research Laboratory, Massachusetts General Hospital, Boston.
Stevens
S
S
Homcy
C J
CJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics* / Q000378:metabolism; D004285:Dogs; D019204:GTP-Binding Proteins / Q000235:genetics*; D015151:Immunoblotting; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D012313:RNA / Q000235:genetics; D013045:Species Specificity
D012313:RNA; D004247:DNA; D019204:GTP-Binding Proteins