A cell mutant that exhibits temperature-dependent sensitivity to transformation by various oncogenes.
We previously isolated a Fisher rat fibroblast mutant, B812, that has the unique property of temperature-dependent transformation by various oncogenic retroviruses. At the permissive temperature (35 degrees C), this mutant was sensitive to oncogenic transformation and formed foci on a dish at the same frequency as did the parental fibroblast cell line. When Kirsten murine sarcoma virus (Ki-MSV) was applied to the cells, the frequency of focus formation decreased more than 25-fold at the nonpermissive temperature (39 degrees C), whereas the cells expressed nearly the same level of the ras transcript as well as the ras protein. The temperature-restricted focus formation was fully reversible and was completely suppressed upon fusion with the wild-type parent cell. In addition to ras, the mos, fos, src, and erbB-2 oncogenes transformed this mutant with the same temperature dependence as described above; polyomavirus middle T antigen, adenovirus type 12, and human papillomavirus 16-E67 also transformed, but without temperature dependence. These results suggest that ras, fos, mos, src, and erbB-2 use a common cellular pathway for transforming cells.
Kizaka
S
S
Research Institute for Microbial Diseases, Osaka University, Japan.
Hakura
A
A
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D015152:Blotting, Northern; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells; D005347:Fibroblasts / Q000166:cytology; D007700:Kinetics; D009154:Mutation; D009857:Oncogenes; D010957:Plasmids; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger; D051381:Rats; D012190:Retroviridae / Q000235:genetics*; D013696:Temperature; D014162:Transfection
D000952:Antigens, Polyomavirus Transforming; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA