T cell activation by purified, soluble, class I MHC molecules. Requirement for polyvalency.
To examine the nature of the interaction of the TCR with the MHC class I Ag, we have studied the stimulation requirements of an H-2Dd-reactive T cell hybridoma, using a homogeneous, purified preparation of a molecularly engineered soluble counterpart of the class I Ag, H-2Dd/Q10b. We demonstrate that this monovalent, soluble MHC Ag is incapable of stimulating the release of IL-2 from this T cell hybridoma. However, the same preparation of the purified protein can elicit a dose-dependent response when made multivalent either by covalent coupling to soluble, high m.w. dextran or to agarose beads, or by adsorption to polystyrene tissue culture plates.
McCluskey
J
J
Molecular Biology Section, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.
Boyd
L F
LF
Highet
P F
PF
Inman
J
J
Margulies
D H
DH
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D000954:Antigens, Surface / Q000235:genetics / Q000276:immunology*; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D003432:Cross-Linking Reagents; D003911:Dextrans; D006183:H-2 Antigens / Q000235:genetics / Q000276:immunology*; D006649:Histocompatibility Antigens / Q000235:genetics / Q000276:immunology*; D015395:Histocompatibility Antigens Class I; D006825:Hybridomas / Q000276:immunology / Q000378:metabolism; D007376:Interleukin-2 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D051379:Mice; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D014162:Transfection
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D003432:Cross-Linking Reagents; D003911:Dextrans; D006183:H-2 Antigens; D006649:Histocompatibility Antigens; D015395:Histocompatibility Antigens Class I; D007376:Interleukin-2; C032907:Q surface antigens; D011948:Receptors, Antigen, T-Cell