Cytokeratin analysis of breast and leukemia tumor cell lines by flow cytometry.
Using four human tumor cell lines, MCF-7 and T47-D from breast tumors, MOLT-4 and K-562 from leukemia, flow cytometric DNA analysis of pure and mixed cell population was performed using monoclonal antibodies to cytokeratin to distinguish cytokeratin-containing carcinoma cells from leukemia cells which do not contain cytokeratins. Surprisingly, on pure or mixed K-562 cells, we found positive labeling with KL1, CK8, and CK18 antibodies (results confirmed by immunocytology). This preliminary study has allowed a DNA analysis on epithelial cells of human breast tumors.
Ferrero
M
M
Laboratoire de Biologie, Centre René Huguenin de Lutte contre le Cancer, Saint-Cloud, France.
Spyratos
F
F
Desplaces
A
A
Andrieu
C
C
Phillips
E
E
Rouëssé
J
J
D000230:Adenocarcinoma; D000911:Antibodies, Monoclonal; D001943:Breast Neoplasms; D002460:Cell Line; D004273:DNA, Neoplasm / Q000032:analysis; D005434:Flow Cytometry; D006801:Humans; D007633:Keratins / Q000378:metabolism*; D007938:Leukemia; D014407:Tumor Cells, Cultured / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D004273:DNA, Neoplasm; D007633:Keratins