title: Histochemical analysis of changes in lectin binding in murine glomerular lesions.
abstract: Lectin binding in diseased murine glomeruli was studied in MRL/1 mice, using seven different fluorescence- or peroxidase-coupled lectins: Griffonia simplicifolia I (GS-I), Ulex europaeus agglutinin I (UEA-I), Ricinus communis agglutinin I (RCA-I), wheat germ agglutinin (WGA), concanavalin A (Con A), peanut agglutinin (PNA), and Helix pomatia agglutinin (HPA). Lectin binding in diseased glomeruli of MRL/1 mice was different from that in normal glomeruli. Light and fluorescence microscopy showed that: 1. in mesangial proliferative lesions, the binding of RCA-I, WGA and Con A increased and that of GS-I and PNA appeared in the mesangium; 2. in other glomerular lesions, UEA-I binding appeared and RCA-I stained the altered membranes irregularly. Electron microscopy showed that: 1. GS-I stained the endothelial cell coat and the glomerular basement membrane covered by the endothelial cells; 2. GS-I strongly stained the dilated subendothelium in regions of mild mesangial interposition; 3. GS-I stained the cell coat of invasive macrophages; 4. GS-I and UEA-I stained the cell membrane-like material derived from degenerative endothelial cells; 5. RCA-I stained the epithelial and endothelial cell coats and the glomerular basement membrane. These results indicate that lectin-binding studies can be used for analysis of glomerular lesions.
authors: 1st Department of Pathology, Kobe University School of Medicine, Japan.
authors: T
authors: T
authors: Kizaki
authors: Z
authors: Z
authors: Takeda
authors: M
authors: M
authors: Watanabe
authors: K
authors: K
authors: Hanioka
authors: H
authors: H
authors: Itoh
mesh_terms: D000818:Animals; D006651:Histocytochemistry; D007674:Kidney Diseases / Q000378:metabolism; D007678:Kidney Glomerulus / Q000378:metabolism* / Q000648:ultrastructure; D037102:Lectins / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D037121:Plant Lectins; D010940:Plant Proteins / Q000378:metabolism; D013194:Staining and Labeling
chemical_list: C045475:Griffonia simplicifolia lectins; D037102:Lectins; D037121:Plant Lectins; D010940:Plant Proteins; C017707:Ricinus communis agglutinin-1; C036850:Ulex europaeus lectins