In vitro treatment of HEp-2 cells with human tumor necrosis factor-alpha and human interferons reduces invasiveness of Salmonella typhimurium.
Enteroinvasive bacteria, like Salmonella typhimurium, can be internalized in in vitro cultured epithelioidal cells, like HEp-2 cells. This phenomenon is inhibited by pretreatment of cells with human tumor necrosis factor alpha (TNF-alpha) in a dose- and time-dependent manner. The effect was also reproduced in other cell types, including diploid embryo fibroblast cells. The TNF-alpha effect was neutralized by anti-TNF-alpha antibodies. No synergistic effect was produced by combinations of TNF-alpha with either interferon alpha or gamma. Unlike the effects of interferons, TNF-alpha inhibited the invasiveness of Shigella flexneri and the TNF-alpha effect was not inhibited by cycloheximide.
Degré
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Kapt. W. Wilhelmsen og frues Bakteriologiske Institutt, Rikshospitalet, University of Oslo, Norway.
Bukholm
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Czarniecki
C W
CW
D002478:Cells, Cultured; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology; D007372:Interferons / Q000494:pharmacology*; D012480:Salmonella Infections / Q000517:prevention & control*; D012486:Salmonella typhimurium / Q000472:pathogenicity; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D014774:Virulence
D007370:Interferon Type I; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D007372:Interferons