Electron microscopic characterisation of retrovirus and retrovirus-like particles induced by demethylating agents (5-azacytidine and 5-azadeoxycytidine) in Syrian hamster (Mesocricetus auratus) cells.
In a framework of a study on retrovirus expression in Syrian hamster (mesocricetus auratus), three cell lines were examined by electron microscopy. As we had previously demonstrated the efficacy of demethylating drug in activating the formation of retroviral particles, we used 5-azacytidine to activate endogenous retrovirus expression. Proceeding to a more detailed survey we were able to observe various types of retrovirions. All of them were present in the different activated cell lines. They were: intracisternal R-type particles (IRP), intracisternal A-type particles (IAP), intracytoplasmic A-type particle (ICAP), intramitochondrial A-type particle (IMAP) and M-432 virus-like particles. Some of these particles have never been described before in Syrian hamster cells. The significance of their presence is discussed here.
Lasneret
J
J
Laboratoire de Virologie des Leucémies-LOI CNRS, Hôpital Saint-Louis, Paris, France.
Lesser
J
J
Dianoux
L
L
Canivet
M
M
Bittoun
P
P
Peries
J
J
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D001374:Azacitidine / Q000031:analogs & derivatives* / Q000494:pharmacology*; D002460:Cell Line; D002478:Cells, Cultured; D006224:Cricetinae; D000077209:Decitabine; D007668:Kidney / Q000166:cytology / Q000382:microbiology; D008647:Mesocricetus; D008854:Microscopy, Electron; D012190:Retroviridae / Q000187:drug effects / Q000648:ultrastructure*; D014407:Tumor Cells, Cultured / Q000473:pathology
D000970:Antineoplastic Agents; D000077209:Decitabine; D001374:Azacitidine