Early screening for anti-plum pox virus monoclonal antibodies with different epitope specificities by means of gold-labelled immunosorbent electron microscopy.
The technique of gold-labelled immunosorbent electron microscopy for the initial screening of monoclonal antibodies 10 days after cell fusion from 96-well culture plates is described. The technique is used to identify clones that secrete antibodies binding on the surface of the virion or to viral subunits, and compared to ELISA and Western blotting. High sensitivity was demonstrated.
Himmler
G
G
Institt für Angewandte Mikrobiologie (IAM), Universitäur Bodenkultur, Vienna, Austria.
Brix
U
U
Steinkellner
H
H
Laimer
M
M
Mattanovich
D
D
Katinger
H W
HW
D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D000956:Antigens, Viral; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D006046:Gold; D007163:Immunosorbent Techniques; D008854:Microscopy, Electron; D010942:Plant Viruses / Q000276:immunology* / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes; D006046:Gold