Isolation and characterization of a functional murine interferon alpha gene which is not expressed in fibroblasts upon virus induction.
A mouse genomic segment containing three new members of the murine interferon alpha (MuIFN-alpha) gene family was isolated from a fibroblastic cosmid library. A 4 kb EcoRI fragment contained a new MuIFN-alpha gene named MuIFN-alpha 8. The nucleotide sequence of the coding and flanking regions of this gene showed a high level of homology to those of known members of the MuIFN-alpha family. Transient expression of the MuIFN-alpha 8 gene in COS cells and oocyte translation of in vitro transcripts both led to a biologically active protein. The antiviral activity was neutralized by monoclonal and polyclonal MuIFN-alpha antibodies. Although the 5' flanking sequence shows features characteristic of an IFN regulatory region, the MuIFN-alpha 8 gene is not expressed in murine fibroblasts treated with Newcastle disease virus or poly(I).poly(C).
Navarro
S
S
Laboratoire de Biologie Moléculaire des Interférons, UPR 37 CNRS, Institut de Recherches Scientifiques sur le Cancer, Villejuif, France.
Dion
M
M
Vodjdani
G
G
Berlot-Picard
F
F
Doly
J
J
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003360:Cosmids; D005347:Fibroblasts; D005786:Gene Expression Regulation; D005796:Genes; D007370:Interferon Type I / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D009522:Newcastle disease virus / Q000502:physiology; D011070:Poly I-C / Q000494:pharmacology; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014721:Vesicular stomatitis Indiana virus / Q000502:physiology
D007370:Interferon Type I; D012313:RNA; D011070:Poly I-C