[mRNA-binding site of ribosomes at different stages of translation. II. Affinity modification of Escherichia coli ribosomes bya benzylidene derivative of AUGU6 in pre- and post-translation complexes].
Affinity labeling of E. coli ribosomes with the 2',3'-O-[4-(N-2-chloroethyl)-N-methyl-amino]benzylidene derivative of AUGU6 (AUGU6-[14C]CHRCl) was studied within the pretranslocational complex ribosome.AUGU6[14C]CHRCl.tRNA(fMet)(P-site).fMetPhe-tR NA(Phe)(A-site) and posttranslocational complex ribosome.AUGU6[14C]CHRCl.fMetPhe-tRNA(Phe)(P-site). Both 30S and 50S subunits were labeled within these complexes, but the extent of 30S subunit modification was 6-8-fold higher than those for 50S subunit. Ribosomal proteins of both subunits were found to be labeled preferentially. Proteins S1, S5, S11, L1 were identified to be crosslinked with AUGU6[14C]CHRCl within the pretranslocational complex and S7--within the posttranslocational complex from the data of two-dimensional electrophoresis in the polyacrylamide gel.
Babkina
G T
GT
Vladimirov
S N
SN
Dryga
S A
SA
Zenkova
M A
MA
Karpova
G G
GG
D000345:Affinity Labels; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D009588:Nitrogen Mustard Compounds; D011089:Polydeoxyribonucleotides; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D012329:RNA, Bacterial / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*
D000345:Affinity Labels; D009588:Nitrogen Mustard Compounds; D011089:Polydeoxyribonucleotides; D012329:RNA, Bacterial; D012333:RNA, Messenger
Issledovanie mRNK-sviazyvaiushcheĭ oblasti ribosomy na raznykh étapakh transliatsii. II. Affinnaia modifikatsiia ribosom Escherichia coli benzilidenovym proizvodnym AUGU6 v sostave pre- i posttranslokatsionnykh kompleksov.