Effects of in vitro incubation on a zona binding site found on murine spermatozoa.
Murine cauda epididymal sperm possess a site, the acceptor, on the plasma membrane over the apical cap region of the acrosome which recognizes both a proteinase inhibitor of seminal vesicle origin and homologous zonae. The acceptor site may participate in both capacitation and zona binding. This presentation explores the effect of in vitro incubation in a medium known to induce capacitation on the binding capabilities of this site. Approximately 80% of fresh cauda epididymal sperm will bind the seminal inhibitor in vitro. Incubating sperm, pretreated with inhibitor for 2 hr in a medium (M199-M) known to support capacitation, reduces by 60% the number of sperm showing evidence of the inhibitor. No such decrease is seen when sperm are incubated in a medium (M199) that does not support capacitation. During the 2-hr incubation in either medium, 60-70% of the sperm retain two diverse components on the plasma membrane over the acrosome: a receptor for the Fc portion of IgG and an epitope recognized by a monoclonal antibody to the acceptor site. These observations suggest that the plasma membrane in the acrosome region of the cell remains structurally intact during incubation. Furthermore, sperm retain the ability to bind the seminal inhibitor during incubation. After a 2-hr incubation in M199-M, sperm pretreated with heat-solubilized zonae no longer bind the inhibitor. These sperm, however, retain the plasma membrane over the acrosomal cap region. When the sperm are incubated in M199, no decrease in inhibitor binding due to zona treatment is noted.(ABSTRACT TRUNCATED AT 250 WORDS)
Boettger
H
H
Department of Biology, University of Alabama, Birmingham 35294.
Richardson
R
R
Free
D
D
Rushing
S
S
Poirier
G R
GR
D000177:Acrosome / Q000276:immunology / Q000378:metabolism; D000818:Animals; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010063:Ovum / Q000378:metabolism*; D011480:Protease Inhibitors / Q000302:isolation & purification / Q000378:metabolism; D011961:Receptors, Fc / Q000032:analysis; D013075:Sperm Capacitation; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000276:immunology / Q000378:metabolism*; D015044:Zona Pellucida / Q000378:metabolism*
D003470:Culture Media; D000939:Epitopes; D011480:Protease Inhibitors; D011961:Receptors, Fc