Idiotypic replica of an anti-human tumor-associated antigen monoclonal antibody. Analysis of monoclonal Ab1 and Ab3 fine specificity.
CaMBr1 is a tissue-specific and tumor-associated saccharidic epitope, defined by mAb MBr1 (Ab1), expressed on glycoconjugates of the human mammary carcinoma cell line MCF-7 and of normal and neoplastic mammary epithelial cells. An anti-anti-idiotypic monoclonal Ab3, 2G-3, identifying a human breast tumor associated antigen, was raised by using as immunogen a mouse anti-idiotypic monoclonal Ab2, A3B10, which behaves as the internal image of CaMBr1. mAb 2G-3, as well as MBr1, defines a saccharidic epitope on glycoconjugates extracted from MCF-7 cells and shows MBr1-like reactivity on normal and neoplastic-tissues. Experimental evidence, however, suggests that the fine immunoreactivity of the two antibodies is not identical, because MBr1 has a preferential reactivity with glycolipids and 2G-3 with glycoproteins. We suggest that a possible biologic explanation for our findings could reside in the nature of the immunogens used to raise the two mAb (glycolipid vs protein "internal image").
Viale
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Dipartimento di Biologia e Genetica per le Scienze Mediche, Università degli Studi, Milano, Italy.
Flamini
G
G
Grassi
F
F
Buffa
R
R
Natali
P G
PG
Pelagi
M
M
Leoni
F
F
Ménard
S
S
Siccardi
A G
AG
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000276:immunology; D000912:Antibodies, Neoplasm / Q000032:analysis* / Q000276:immunology; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000276:immunology*; D002460:Cell Line; D002474:Cell-Free System; D000939:Epitopes / Q000276:immunology; D006801:Humans; D006825:Hybridomas / Q000032:analysis; D007106:Immune Sera / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000032:analysis / Q000276:immunology*; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D014407:Tumor Cells, Cultured
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D000939:Epitopes; D007106:Immune Sera; D007130:Immunoglobulin Idiotypes