title: Voltage-sensitive ion channel of Escherichia coli.
abstract: A voltage-sensitive, cation-selective ion channel of Escherichia coli has been reconstituted into liposomes and studied with the patch-clamp method. The single channel conductance was 91 pS in symmetric solutions of 150 mM KCl. Many channels were open most of the time, with frequent brief transitions to closed levels. Multiple conducting units could close and reopen simultaneously, and this apparent cooperativity in gating was increased with depolarizing voltages. Above a voltage threshold, the channels closed irreversibly, often in groups.
authors: Department of Biochemistry, University of Wisconsin, Madison 53706.
authors: A H
authors: AH
authors: Delcour
authors: B
authors: B
authors: Martinac
authors: J
authors: J
authors: Adler
authors: C
authors: C
authors: Kung
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D003627:Data Interpretation, Statistical; D004558:Electric Stimulation; D004926:Escherichia coli / Q000378:metabolism*; D007473:Ion Channels / Q000378:metabolism*; D008081:Liposomes; D008564:Membrane Potentials
chemical_list: D001425:Bacterial Outer Membrane Proteins; D007473:Ion Channels; D008081:Liposomes