Characterization of airway mucus from a fatal case of status asthmaticus.
We used density gradient methods to analyze mucus recovered from the airways of a patient who died from status asthmaticus. At postmortem the mucus that plugged the length of the bronchial tree could only be removed by cutting with forceps. It was dispersed in cesium bromide (CsBr) and examined in the analytical ultracentrifuge. Surprisingly, no macromolecular component was seen in the buoyant density region typical of mucus glycoprotein (at 1.5 g/ml). The gelatinous material migrated to the region of lowest density, typical of lipids, and thin-layer chromatography indicated that besides neutral lipids, a variety of phospho- and glycolipids (including gangliosides) were present. The residue left after the lipid extraction contained all sugar components of mucus glycoprotein, and alkaline borohydride cleaved oligosaccharides three to 12 sugars long, indicating that the sugars were O-glycosidically linked as in mucus glycoproteins. Prior desulfation of the residue increased the number of oligosaccharides released by borohydride; analysis showed the presence in these of components (glucose, methylmannuronate) not present in epithelial glycoprotein. A trace component of high buoyant density was also present: Glucose was the predominant component in this, but glycoprotein sugars were also present. The above results suggest that in status asthmaticus plugging of the airways with mucus can occur even in the absence of typical epithelial glycoprotein and that lipids play a crucial role in such plugging.
Bhaskar
K R
KR
Department of Pathology, Harvard Medical School, Boston, Massachusetts.
O'Sullivan
D D
DD
Coles
S J
SJ
Kozakewich
H
H
Vawter
G P
GP
Reid
L M
LM
D000293:Adolescent; D000402:Airway Obstruction / Q000209:etiology / Q000473:pathology; D001249:Asthma / Q000378:metabolism*; D002499:Centrifugation, Density Gradient / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D006023:Glycoproteins / Q000032:analysis; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D009093:Mucus / Q000032:analysis*; D012137:Respiratory System / Q000473:pathology; D013194:Staining and Labeling; D013224:Status Asthmaticus / Q000378:metabolism* / Q000473:pathology
D006023:Glycoproteins