title: Yeast mutation thought to arrest mRNA transport markedly increases the length of the 3' poly(A) on polyadenylated RNA.
abstract: In Saccharomyces cerevisiae the function of the RN A1 gene is believed to be required for the transport of newly synthesized mRNA from the nucleus to the cytoplasm. Nuclear poly(A)+ RNAs accumulate and cytoplasmic mRNAs decay after the temperature-sensitive (ts) rna1.1 mutant is shifted from 25 degrees C to 37 degrees C. In this study the 3' poly(A) upon poly(A)+ RNA synthesized after expression of rna1.1 was shown to be appreciably longer than the poly(A) normally present on yeast cytoplasmic mRNA. This increased poly(A) length is due to rna1.1, since it was found only in this mutant after a 25 degrees C to 37 degrees C heat shock, not an intragenic non-ts revertant of rna1.1, wild-type (RN A1+) cells or a RN A1+, rna2.1 mutant subjected to equivalent heat shocks. It may be an indication that the normal shortening of the poly(A) on mRNAs does not occur in the nucleus, but happens only with transport to the cytoplasm. Alterations in the mean size of poly(A) may be a relatively simple marker for mRNA transport defects.
authors: Department of Biochemistry, University College London, U.K.
authors: P W
authors: PW
authors: Piper
authors: J L
authors: JL
authors: Aamand
mesh_terms: D001692:Biological Transport; D003593:Cytoplasm; D009154:Mutation; D011061:Poly A / Q000235:genetics*; D012313:RNA / Q000235:genetics*; D012331:RNA, Fungal; D012333:RNA, Messenger / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D012331:RNA, Fungal; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA