Effects of interferons, interferon inducers and growth factors on phagocytosis measured by quantitative determination of synthetic compound ingested by mouse bone marrow-derived macrophages.
Sodium salt of 9-oxo-10-acridineacetohydroxamic acid (HCA), a new synthetic compound, forms small crystals in aqueous solution. These crystals were easily phagocytized by the mouse bone marrow-derived macrophages. The ingested HCA crystals were visible under light microscope as dark granules. The degree of phagocytosis was estimated by the spectroscopic measurements of absorption of ingested HCA. About 10 day-old cultures of mouse bone marrow-derived macrophages were found to be suitable for a study on the effect of murine or human interferons. It was observed that murine interferons alpha, beta and gamma at low concentrations (10-200 U/ml) stimulated and higher concentrations (400-1000 U/ml) had no effect on the phagocytosis. Previous treatment of interferons with anti-IFN sera abolished the effect of the interferons. CMA-induced interferon and growth factors were found to modify the phagocytic activity of macrophage cultures.
Szulc
B
B
Laboratory of Tumor Virology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.
Piasecki
E
E
D000166:Acridines / Q000494:pharmacology; D000818:Animals; D001854:Bone Marrow Cells; D002954:Citrobacter / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology; D004768:Enterotoxins / Q000494:pharmacology; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects; D006133:Growth Substances / Q000494:pharmacology; D006877:Hydroxamic Acids / Q000494:pharmacology; D007369:Interferon Inducers / Q000494:pharmacology*; D007370:Interferon Type I / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology; D007372:Interferons / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010587:Phagocytosis / Q000187:drug effects*; D010835:Phytohemagglutinins / Q000494:pharmacology; D015291:Transforming Growth Factors / Q000494:pharmacology
D000166:Acridines; D004768:Enterotoxins; D006133:Growth Substances; D006877:Hydroxamic Acids; D007369:Interferon Inducers; D007370:Interferon Type I; D010835:Phytohemagglutinins; D003208:Concanavalin A; C059943:9-oxo-10-acridineacetohydroxamic acid; C026066:enterotoxin A, Staphylococcal; D004815:Epidermal Growth Factor; D015291:Transforming Growth Factors; D007371:Interferon-gamma; D007372:Interferons