title: Assessment of selectivity of muscarinic antagonists for M1 and M2 receptors in rabbit isolated vas deferens.
abstract: Neurogenic contractions of the rabbit isolated vas deferens were inhibited by McN-A-343 but potentiated by carbachol. The actions of McN-A-343 and carbachol on the twitch contractions were antagonized to different degrees by antimuscarinic drugs: the affinity (pA2) of atropine and himbacine against McN-A-343 and carbachol was similar. However, pirenzepine and telenzepine displayed preferential antagonism of McN-A-343 responses, whereas the converse was true for methoctramine and AF-DX 116. The pA2 values of 6 antagonists against carbachol responses in rabbit vas deferens closely agree with those obtained in rat left atrium. In unstimulated organs carbachol and McN-A-343 had no effect on the resting tension. Contractions elicited by ATP, noradrenaline and KCl were potentiated by carbachol but were unaffected by McN-A-343. The data suggest the presence of a presynaptic M1 receptor mediating inhibition and a postsynaptic, cardiac-like M2 receptor enhancing neurogenic contractions in rabbit vas deferens.
authors: Byk Gulden Research Laboratories, Konstanz, FRG.
authors: M
authors: M
authors: Eltze
mesh_terms: D008455:(4-(m-Chlorophenylcarbamoyloxy)-2-butynyl)trimethylammonium Chloride / Q000494:pharmacology; D000818:Animals; D016275:Atrial Function; D002217:Carbachol / Q000037:antagonists & inhibitors / Q000494:pharmacology; D004558:Electric Stimulation; D006325:Heart Atria / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D010276:Parasympatholytics / Q000494:pharmacology*; D011817:Rabbits; D011976:Receptors, Muscarinic / Q000187:drug effects* / Q000502:physiology; D014649:Vas Deferens / Q000187:drug effects* / Q000502:physiology
chemical_list: D010276:Parasympatholytics; D011976:Receptors, Muscarinic; D008455:(4-(m-Chlorophenylcarbamoyloxy)-2-butynyl)trimethylammonium Chloride; D002217:Carbachol