[AFP gene expression and antigenic protein localization in the cells of hepatocellular carcinoma and its surrounding tissues].
Using a double immunoenzymatic technique (PAP) and AFPcDNA-RNA in situ hybridization technique, we detected and analyzed the AFP gene expression and antigenic protein localization in hepatocellular carcinoma (HCC) and its surrounding tissues. The results demonstrated that AFP was truly synthesized again by some host liver cells around the cancer nodules. We considered that the cells which showed AFPmRNA positive hybridized signs might be a kind of preneoplastic cells. Their appearance might be related to injury caused by HBV infection and the inactivities of the inhibited gene on the AFP control mechanism. The substances secreted by the involved cancer cells, might be another causative factor for the excessive expression of the AFP in the liver cells near the cancer.
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D000293:Adolescent; D000328:Adult; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002648:Child; D005260:Female; D015870:Gene Expression; D005796:Genes; D006801:Humans; D007120:Immunochemistry; D008099:Liver / Q000378:metabolism; D008113:Liver Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger; D000509:alpha-Fetoproteins / Q000235:genetics*
D012333:RNA, Messenger; D000509:alpha-Fetoproteins