Restricted use of T cell receptor V genes in murine autoimmune encephalomyelitis raises possibilities for antibody therapy.
Experimental allergic encephalomyelitis (EAE) is a paralytic autoimmune disease induced in susceptible animals by active immunization with myelin basic protein (MBP) or by passive transfer of MBP-specific T helper (TH) lymphocytes. We have analyzed the T cell receptor genes of 33 clonally distinct TH cells specific for a nonapeptide of MBP inducing EAE in B10.PL (H-2u) mice. All 33 TH cells used two alpha variable gene segments (V alpha 2.3, 61%; V alpha 4.2, 39%), the same alpha joining gene segment (J alpha 39), and two V beta and J beta gene segments (V beta 8.2-J beta 2.6, 79%; V beta 13-J beta 2.2, 21%). The anti-V beta 8 monoclonal antibody F23.1 was found to block completely recognition of the nonapeptide by V beta 8 TH cells in vitro and to reduce significantly the susceptibility of B10.PL mice to peptide-induced EAE.
Urban
J L
JL
Division of Biology, California Institute of Technology Pasadena 91125.
Kumar
V
V
Kono
D H
DH
Gomez
C
C
Horvath
S J
SJ
Clayton
J
J
Ando
D G
DG
Sercarz
E E
EE
Hood
L
L
D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000235:genetics*; D000939:Epitopes; D005434:Flow Cytometry; D006825:Hybridomas; D007114:Immunization; D008198:Lymph Nodes; D051379:Mice; D008969:Molecular Sequence Data; D004676:Myelin Basic Protein / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D004676:Myelin Basic Protein; D011948:Receptors, Antigen, T-Cell