Substance P modulates lymphokine activities in supernatants of cultured human duodenal biopsies.
A duodenal biopsy culture technique was used to investigate the effect of substance P on lymphokine secretion by the human gut associated lymphoid tissue. Duodenal biopsies of 7 healthy volunteers were cultured in 1 ml medium each with Pokeweed mitogen (1 microgram/ml) for 4 days at 37 degrees C. Substance P (SP) was added in concentrations ranging from 10(-12) M to 10(-6) M. Media were changed every day. Interleukin (IL)-1 beta, IL-2 and IL-2-receptor activities were determined by means of specific ELISAs. Values were referred to 5 mg biopsy weight and expressed as per cent change of basal Pokeweed mitogen-pulsed supernatant activities. 10(-8) M and 10(-6) M SP led to a decrease of IL-1 beta activity (78 +/- 13.9% and 62.8 +/- 17.1%, respectively, alpha = 0.01 each). In contrast, 10(-8) and 10(-10) M SP showed an increase in IL-2 activity up to 182.9 +/- 94.5% and 295.6 +/- 144.7%, respectively. 10(-6) M and 10(-8) M SP enhanced IL-2 receptor activities by 81.5 +/- 70% and 40.9 +/- 11.8%, respectively (alpha = 0.05). The present data demonstrate for the first time distinct SP-mediated effects on lymphokine activities in supernatants of cultured human duodenal biopsies.
Hart
R
R
IInd Department of Medicine, Technical University, Munich, F.R.G.
Dancygier
H
H
Wagner
F
F
Niedermeyer
H
H
Classen
M
M
D000276:Adjuvants, Immunologic; D046508:Culture Techniques; D004386:Duodenum / Q000187:drug effects / Q000276:immunology*; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D008221:Lymphoid Tissue / Q000187:drug effects / Q000276:immunology; D008222:Lymphokines / Q000378:metabolism*; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis; D013373:Substance P / Q000494:pharmacology*
D000276:Adjuvants, Immunologic; D007375:Interleukin-1; D007376:Interleukin-2; D008222:Lymphokines; D015375:Receptors, Interleukin-2; D013373:Substance P