A B72.3 second-generation-monoclonal antibody (CC49) defines the mucin-carried carbohydrate epitope Gal beta(1-3) [NeuAc alpha(2-6)]GalNAc.
Mucus glycoproteins from bovine seminal plasma were demonstrated to express the MAbs CC49- and B72.3-defined epitopes on TAG 72 antigen. Inhibition studies with reductively cleaved mucin O-glycans from bovine seminal plasma and submaxillary glands revealed that CC49 binds specifically to a core-type sialyl-oligosaccharide alditol (fraction 2c), which could be defined with regard to its primary structure by FAB- and EI-mass spectrometry combined with methylation analysis: (formula; see text) Structurally related alditols NeuAc alpha(2-6)-GalNAc-ol, Gal beta(1-3)GalNAc-ol, NeuAc alpha(2-3)-Gal beta(1-3) [NeuAc alpha(2-6)] GalNAc-ol, GlcNAc beta(1-3) [NeuAc alpha(2-6)] GalNAc-ol or NeuAc alpha(2-3) Gal beta(1-3)GalNAc-ol were not inhibitory.
Hanisch
F G
FG
Abt. Immunbiologie, Universitätsklinik Köln.
Uhlenbruck
G
G
Egge
H
H
Peter-Katalinić
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000276:immunology; D002241:Carbohydrates / Q000276:immunology; D002417:Cattle; D000939:Epitopes / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D009077:Mucins / Q000032:analysis / Q000276:immunology*; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000032:analysis; D029589:Seminal Plasma Proteins; D013363:Submandibular Gland / Q000032:analysis; D013402:Sugar Alcohols / Q000276:immunology; D014312:Trisaccharides
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D002241:Carbohydrates; D000939:Epitopes; D006023:Glycoproteins; D009077:Mucins; D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; D013402:Sugar Alcohols; D014312:Trisaccharides; C043076:beta-microseminoprotein; C047052:tumor-associated antigen 72