title: Effects of cyclic nucleotides and calcium/calmodulin on protein phosphorylation in the CNS of Hirudo medicinalis.
abstract: Protein phosphorylation plays an important role in the regulation of neural functions. We have studied the phosphorylation of proteins in homogenates of segmental ganglia of the leech Hirudo medicinalis. We describe a number of proteins whose phosphorylation is dependent on calcium/calmodulin or cyclic nucleotides. Most of the proteins whose phosphorylation is increased in the presence of calcium seem to be substrates for cyclic nucleotide-dependent protein kinases. Only two of the phosphoproteins described appear to be specific substrates for calcium/calmodulin protein kinase(s), and at least six phosphoproteins appear to be specific substrates for cyclic nucleotide-dependent kinase(s). The leech nervous system, with large and identifiable neurons, provides a good tool for studies of neural functions, such as learning. The results are discussed in the context of the role of protein phosphorylation on learning processes.
authors: Department of Physiology and Biochemistry, University of Pisa, Italy.
authors: M
authors: M
authors: Garcia-Gil
authors: F
authors: F
authors: Berton
authors: E
authors: E
authors: Tongiorgi
authors: M
authors: M
authors: Brunelli
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002147:Calmodulin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000494:pharmacology*; D006152:Cyclic GMP / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D007865:Leeches / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009420:Nervous System / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D012969:Sodium Fluoride / Q000494:pharmacology
chemical_list: D002147:Calmodulin; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D012969:Sodium Fluoride; D000242:Cyclic AMP; D011494:Protein Kinases; D006152:Cyclic GMP; D002118:Calcium; D015056:1-Methyl-3-isobutylxanthine