title: Interleukin 3-dependent and -independent mast cells stimulated with IgE and antigen express multiple cytokines.
abstract: In response to IgE and specific multivalent antigen, mast cell lines (both growth factor-dependent and -independent) induce the transcription and/or secretion of a number of cytokines having a wide spectrum of activities. We have identified IL-1, IL-3, IL-5, IL-6, IFN-gamma, GM-CSF, JE, MIP1 alpha, MIP1 beta, and TCA3 RNA in at least two of four mast cell clones. The production of these products (except JE) is activation-associated and can be induced by IgE plus antigen. In selected instances cytokine expression can also be induced by activation with Con A or phorbol ester plus ionophore, albeit to levels less than those observed with IgE plus antigen. In addition, long-term mast cell clones and primary cultures of bone marrow-derived mast cells specifically release IL-1, IL-4, and/or IL-6 bioactivity after activation. These findings suggest that in addition to their inflammatory effector function mast cells may serve as a source of growth and regulatory factors. The relationship of mast cells to cells of the T lymphocyte lineage is discussed.
authors: Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115.
authors: P R
authors: PR
authors: Burd
authors: H W
authors: HW
authors: Rogers
authors: J R
authors: JR
authors: Gordon
authors: C A
authors: CA
authors: Martin
authors: S
authors: S
authors: Jayaraman
authors: S D
authors: SD
authors: Wilson
authors: A M
authors: AM
authors: Dvorak
authors: S J
authors: SJ
authors: Galli
authors: M E
authors: ME
authors: Dorf
mesh_terms: D000818:Animals; D000944:Antigens, Differentiation, B-Lymphocyte / Q000276:immunology*; D001685:Biological Factors / Q000096:biosynthesis / Q000235:genetics*; D015152:Blotting, Northern; D002460:Cell Line; D002478:Cells, Cultured; D002999:Clone Cells; D016207:Cytokines; D015342:DNA Probes; D005786:Gene Expression Regulation; D007073:Immunoglobulin E / Q000276:immunology*; D007377:Interleukin-3 / Q000276:immunology*; D008407:Mast Cells / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D011961:Receptors, Fc / Q000276:immunology*; D017455:Receptors, IgE
chemical_list: D000944:Antigens, Differentiation, B-Lymphocyte; D001685:Biological Factors; D016207:Cytokines; D015342:DNA Probes; D007377:Interleukin-3; D011961:Receptors, Fc; D017455:Receptors, IgE; D007073:Immunoglobulin E; D012313:RNA