The alterations of magnetic resonance relaxation parameters in excised myocardial tissue during NMR spectroscopy: the effects of time, environmental exposure and TTC staining.
To investigate the potential impact of air exposure, time delay and vital tissue staining on the MR relaxation parameters in normal and pathological heart tissues, myocardial samples from a canine model of myocardial infarction were subject to (a) air exposure for up to 20 hours or (b) wrapping in Parafilm for up to 20 hours; immersion in (c) full strength pathological stain consisting of triphenyl tetrazolium chloride (TTC), or (d) half strength TTC, or (e) normal saline for 30 minutes. We found that (a) exposure to air produced rapid change in both T1 and T2 such that there is a reduction of T1 by 12.2% and T2 by 14.4% (p less than 0.001) in one hour; (b) airtight wrapping attenuated dramatically these changes, but T1 still was reduced by 2.9% and T2 by 4.8% in one hour (p less than 0.01). These changes followed similar but non-linear changes of tissue water content. T1 did not change significantly after exposure to full strength TTC, but did increase significantly after exposure to half strength TTC, and increased even further after exposure to saline. T2 on the other hand increased significantly with all of these test solutions. We conclude that the in vitro processing of excised myocardial tissues should be done by wrapping in an airtight container with T1 and T2 parameters measured within one hour, if possible. All tissue processing, including stains and saline exposure, should be done after spectrometer measurements.
Liu
P
P
Department of Radiology, Massachusetts General Hospital, Boston.
Johnston
D L
DL
Brady
T J
TJ
Lutrario
D M
DM
Okada
R D
RD
D000388:Air; D000818:Animals; D004285:Dogs; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D009203:Myocardial Infarction / Q000175:diagnosis; D009206:Myocardium / Q000473:pathology*; D012965:Sodium Chloride; D013194:Staining and Labeling; D013778:Tetrazolium Salts; D013997:Time Factors
D013778:Tetrazolium Salts; D012965:Sodium Chloride; C009591:triphenyltetrazolium