Synthesis of gelatin microspheres containing interferon.
Gelatin microspheres with a diameter less than 2 microns were synthesized by means of cross-linking with glutaraldehyde. When the microspheres were subjected to degradation in phosphate-buffered saline solution containing collagenase, the digestion of microspheres was found to decrease with increasing cross-linking. Interferon was incorporated in the microspheres at a high trapping efficiency, and the rate of interferon release from the microspheres was regulated by the extent of cross-linking with glutaraldehyde. Gelatin microspheres incorporating interferon-alpha were readily phagocytosed by macrophages, regardless of the extent of cross-linking, and the phagocytosed microspheres were observed to be degraded gradually in the interior of macrophages, resulting in the slow release of the incorporated interferon in the cells.
Tabata
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Research Center for Medical Polymers and Biomaterials, Kyot University, Japan.
Ikada
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Y
D002626:Chemistry, Pharmaceutical; D005780:Gelatin; D005976:Glutaral; D007202:Indicators and Reagents; D007372:Interferons / Q000008:administration & dosage* / Q000032:analysis / Q000494:pharmacology; D007457:Iodine Radioisotopes; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010587:Phagocytosis / Q000187:drug effects; D013997:Time Factors
D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D005780:Gelatin; D007372:Interferons; D005976:Glutaral