ADP effects on bleomycin-induced DNA repair synthesis and adenylate kinase activity in permeable mouse sarcoma cells.
DNA repair in bleomycin-pretreated, permeable mouse sarcoma (SR-C3H/He) cells requires ATP for at least two steps, the repair DNA synthesis step and the repair patch ligation step. ADP can apparently replace ATP in both steps. Maximal, 1.5-2 fold stimulation of repair DNA synthesis was observed with 5-10 mM ADP as well as 2.5-5 mM ATP. Repair patch ligation in the presence of 2.5 mM ADP occurred at almost the same high efficiency as it did in the presence of ATP. The ADP effect on DNA repair patch ligation was attributed to ATP formed from ADP by adenylate kinase in permeable cells, however the ADP effect on repair DNA synthesis could not be attributed solely to the formation of ATP in the same manner.
Seki
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S
Department of Biochemistry, Cancer Institute, Okayama University Medical School, Japan.
Mori
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S
Oda
T
T
D000244:Adenosine Diphosphate / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000263:Adenylate Kinase / Q000378:metabolism*; D000818:Animals; D001761:Bleomycin / Q000494:pharmacology*; D002463:Cell Membrane Permeability; D004260:DNA Repair / Q000187:drug effects*; D004261:DNA Replication / Q000187:drug effects*; D003902:Detergents; D007700:Kinetics; D051379:Mice; D008809:Mice, Inbred C3H; D017830:Octoxynol; D010770:Phosphotransferases / Q000378:metabolism*; D011092:Polyethylene Glycols; D012513:Sarcoma, Experimental / Q000201:enzymology / Q000235:genetics*
D003902:Detergents; D001761:Bleomycin; D011092:Polyethylene Glycols; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D017830:Octoxynol; D010770:Phosphotransferases; D000263:Adenylate Kinase