Use of an antigen-capture assay for characterisation of monoclonal antibodies to mycobacterial lipoarabinomannan.
Monoclonal antibodies directed to six separate antigen molecules of Mycobacterium leprae have been tested in an antigen-capture assay based on combined use of polyclonal ("capture") and monoclonal ("detector") antibody reagents. This approach provides a potentially versatile, sensitive and specific assay for detection and relative quantitation of M. leprae antigens. Characterisation of monoclonal antibodies to mycobacterial lipoarabinomannan (LAM-B) by the antigen-capture assay indicates that some of the antigenic determinants present on LAM-B from M. leprae may be either absent altogether or present at much lower concentrations on the corresponding LAM-B structure form M. tuberculosis.
Khanolkar
S R
SR
Department of Clinical Tropical Medicine, London School of Hygiene, London.
Young
D B
DB
Brennan
P J
PJ
Buchanan
T M
TM
McAdam
K P
KP
D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology*; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology*; D009166:Mycobacterium leprae / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000276:immunology; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes; D008070:Lipopolysaccharides; C050016:lipoarabinomannan