[Histopathologic and cytochemical studies of the kidneys of guinea pigs infected with larvae of Ascaris suum Goeze, 1782].
The material consisted of 20 male guinea pigs of body weight 300 +/- 50 g. Fifteen of them were infected per os with a dose of 5000 invasive eggs of A. suum. At the 4th, 7th and 20th days of infection the animals were dissected and sections of kidney were taken for histopathological and histochemical studies. The sections were stained with hematoxylin and eosin. The presence of mucopolisachcarides was detected with the paS method, the activity of acid and alkaline phosphatases--with Gomori's method. At the 4th and 7th day of infection an active congestion of the kidney, dilation of blood vessels and changes in the size of epithelial cells of proximal concoluted tubules were observed. The epithelium of the tubules was high, often closing their lumen. Moreover, at the 7th day of infection small ecchymosis was noted. On the sections stained with the paS method a distinct thickening of the basement membrane of the parietal lamellat of Bowman sac was found at the 4th and 7th day of infection, as well as a weaker staining of brush border in main parts of nephrons. A tightening of the vasculary loop of some glomeruli was also noted at the 7th day. At the 20th day of the experiment changes were observed only in single glomeruli in the vasculary loop. At the 4th day of infection an increase in the activity of the acid and alkaline phosphatases was observed in the kidney, followed by a decrease in the activity of both these enzymes at the 7th and 20th day.
Rukasz
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D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001196:Ascariasis / Q000378:metabolism / Q000473:pathology*; D006025:Glycosaminoglycans / Q000378:metabolism; D006168:Guinea Pigs; D006651:Histocytochemistry; D007668:Kidney / Q000378:metabolism / Q000473:pathology*; D008297:Male; D013194:Staining and Labeling
D006025:Glycosaminoglycans; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase
Badania histopatologiczne i cytochemiczne nerki świnek morskich zarazonych larwami Ascaris suum Goeze, 1782.