title: Cytochemical changes in a human arterial proteoglycan related to atherosclerosis.
abstract: The cuprolinic blue (CB) staining method has been used to visualize and characterize proteoglycans (PG) in the extracellular matrix (ECM) of normal and atherosclerotic human arteries. Arterial tissues of 13 individuals (1-83 years of age) were obtained by autopsy. For electron microscopic visualization of PGs staining with CB was performed in the presence of a critical electrolyte concentration of 0.3 M MgCl2. Under these conditions CB selectively interacts with the polysulfated glycosaminoglycan (GAG) side chains of the molecules. Removal of PG side chains by GAG-degrading enzymes prior to CB staining selectively prevented the formation of chondroitin sulfate (CS)-rich and dermatan sulfate (DS)-rich PG-CB precipitates. The DS-rich type of PG is mainly associated with collagen fibrils, the CS-rich type of PG is preferentially localized in nonfibrous areas of the ECM (soluble matrix). When normal arterial tissues are compared with those affected by atherosclerosis quantitative and qualitative changes of PG-CB precipitates are detected. In fibrous plaques a strong accumulation of a large CS-rich type of precipitate close by smooth muscle cells (SMC) and foam cells is observed. In addition, these precipitates are significantly longer in fibrous plaques than in adjacent normal media (116 nm vs. 100 nm; P less than 0.001). This alteration is independent of the age of the donor. Small DS-rich PG-CB precipitates associated with collagen fibrils show strong variations in their length, but not a significant tendency towards elongated precipitates in atherosclerosis. The present results demonstrate that ultracytochemical and morphometric analysis are useful in providing information on the diverse types, locations, interactions, and possibly of molecular changes of PGs in normal and atherosclerotic human arteries.
authors: Institute for Arteriosclerosis Research, University of Münster, F.R.G.
authors: W
authors: W
authors: Völker
authors: A
authors: A
authors: Schmidt
authors: E
authors: E
authors: Buddecke
mesh_terms: D001158:Arteries / Q000648:ultrastructure*; D001161:Arteriosclerosis / Q000378:metabolism*; D001344:Autopsy; D006651:Histocytochemistry; D006801:Humans; D008854:Microscopy, Electron; D011509:Proteoglycans / Q000378:metabolism*; D013194:Staining and Labeling
chemical_list: D011509:Proteoglycans