Bile canalicular contraction in the isolated hepatocyte doublet is related to an increase in cytosolic free calcium ion concentration.
Dynamic contractions of bile canaliculi have been observed in cultured doublet hepatocytes by means of time-lapse cinephotomicrography, and this contractile movement plays an important role in bile secretion. Although details of the mechanism are still unknown, the Ca2+-calmodulin system is believed to play a main role in this mechanism. In this study we measured the intracellular Ca2+ concentration of individual doublet hepatocytes using the Ca2+ indicator "fura 2" and microscopic fluorometry. We also observed the effects of A23187, norepinephrine and epinephrine on bile canalicular contraction and intracellular Ca2+ concentration. After loading 1 mumol/l fura 2 in doublet cells, we added A23187, epinephrine or norepinephrine and then measured the Ca2+ concentration in a given small area in the cytoplasm of individual doublet cell. A23187, norepinephrine and epinephrine caused a prompt increase of the intracellular Ca2+ concentration and also caused bile canalicular contraction. The present study indicates that the sudden increase of intracellular Ca2+ concentration causes bile canalicular contraction through the Ca2+-calmodulin system.
Watanabe
S
S
Department of Medicine, Juntendo University, Tokyo, Japan.
Tomono
M
M
Takeuchi
M
M
Kitamura
T
T
Hirose
M
M
Miyazaki
A
A
Namihisa
T
T
D000818:Animals; D001648:Bile Canaliculi / Q000187:drug effects / Q000502:physiology*; D001653:Bile Ducts, Intrahepatic / Q000502:physiology*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D004837:Epinephrine / Q000494:pharmacology; D005260:Female; D007473:Ion Channels / Q000502:physiology*; D008099:Liver / Q000166:cytology*; D009638:Norepinephrine / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains
D007473:Ion Channels; D000001:Calcimycin; D002118:Calcium; D009638:Norepinephrine; D004837:Epinephrine