Alpha B-crystallin is expressed in kidney epithelial cell lines and not in fibroblasts.
We have recently shown the presence of alpha B-crystallin in non-ocular tissues of diverse embryological origins such as the heart, brain, spinal cord, kidney, retina, etc. Using an alpha B-crystallin-specific antiserum and immunofluorescence, immunoblotting, immunoprecipitation and peptide mapping with Staphylococcus aureus protease, we demonstrate differential expression of alpha B-crystallin in epithelial and fibroblast cell lines. alpha B-Crystallin was detectable only in epithelial cell lines such as MDBK, MDCK, LLCPK1 and JTC-12, and was not observed in two kidney fibroblast cell lines, one skin fibroblast cell line, and one corneal fibroblast cell line. Differential expression of the alpha B-crystallin gene was also confirmed by Northern blot analysis of the RNAs isolated from these cell lines. These data suggest a cell-type-specific role for alpha B.
Nagineni
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Jules Stein Eye Institute, University of California, School of Medicine, Los Angeles 90024-1771.
Bhat
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D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D003315:Cornea; D003459:Crystallins / Q000032:analysis* / Q000235:genetics; D010450:Endopeptidases; D004848:Epithelium / Q000032:analysis; D005333:Fetus; D005347:Fibroblasts / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006801:Humans; D007668:Kidney / Q000032:analysis* / Q000166:cytology; D010449:Peptide Mapping; D011233:Precipitin Tests; D012313:RNA / Q000032:analysis; D012867:Skin
D003459:Crystallins; D012313:RNA; D010450:Endopeptidases