title: One-step purification of murine IgM and human alpha 2-macroglobulin by affinity chromatography on immobilized snowdrop bulb lectin.
abstract: A new mannose-specific plant lectin (GNA) isolated from the snowdrop bulb was immobilized on Sepharose 4B and employed for the purification of certain glycoproteins with high-mannose type glycan chains. Murine IgM bound tightly to this column and was eluted with 0.1 M methyl alpha-D-mannoside whereas bovine and murine IgG were not bound. When a murine hybridoma serum containing IgM monoclonal antibody was applied to this column, highly purified IgM antibody was obtained after elution with methyl alpha-D-mannoside. On the contrary, human IgM was not bound by this column despite reports that it contains high-mannose type glycan chains. alpha 2-Macroglobulin was the sole glycoprotein present in human serum which was bound by the immobilized snowdrop lectin column. It appears that only glycoproteins containing multiple Man(alpha 1,3)Man units are bound to the immobilized lectin.
authors: Department of Biological Chemistry, University of Michigan, Ann Arbor 48109.
authors: N
authors: N
authors: Shibuya
authors: J E
authors: JE
authors: Berry
authors: I J
authors: IJ
authors: Goldstein
mesh_terms: D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007122:Immunoelectrophoresis; D007075:Immunoglobulin M / Q000302:isolation & purification*; D037102:Lectins; D051379:Mice; D012685:Sepharose; D000511:alpha-Macroglobulins / Q000302:isolation & purification*
chemical_list: D007075:Immunoglobulin M; D037102:Lectins; D000511:alpha-Macroglobulins; D012685:Sepharose