Activation of platelet phospholipase C by fluoride is inhibited by elevation of cyclic AMP.
Fluoride interacts with G proteins and, consequently, stimulates phospholipase C as measured by the formation of inositol phosphates and phosphatidic acid. In human platelets this paralleled platelet aggregation and the activation of phosphorylation of substrates of protein kinase C (47kDa protein) and myosin light chain kinase (20kDa protein). Phospholipase C activation by fluoride was inhibited by dibutyryl cyclic AMP and by agents that increase cyclic AMP levels such as iloprost and forskolin. This information suggest that cyclic AMP affects the G protein associated with the stimulation of phospholipase C.
Lazarowski
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ER
Division of Cell Biology, Burroughs Wellcome Co., Research Triangle Park, NC 27709.
Lapetina
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EG
D001792:Blood Platelets / Q000201:enzymology*; D003994:Bucladesine / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D011464:Epoprostenol / Q000494:pharmacology; D005459:Fluorides / Q000494:pharmacology*; D006801:Humans; D016285:Iloprost; D066298:In Vitro Techniques; D010712:Phosphatidic Acids / Q000096:biosynthesis; D010716:Phosphatidylinositols / Q000378:metabolism; D010974:Platelet Aggregation / Q000187:drug effects; D010738:Type C Phospholipases / Q000378:metabolism*
D010712:Phosphatidic Acids; D010716:Phosphatidylinositols; D005576:Colforsin; D003994:Bucladesine; D011464:Epoprostenol; D000242:Cyclic AMP; D010738:Type C Phospholipases; D016285:Iloprost; D005459:Fluorides