Leptospiral lipopolysaccharide presence in the outer envelope: electrophoretic evidence and immunological specificity.
Purified preparations of lipopolysaccharides (LPS) extracted from two different strains of Leptospira interrogans have been electrophoretically analyzed in order to determine their location at the level of outer envelope (OE). Evidence has been collected for the presence of some LPS fractions in the OE, suggesting that a part of this molecule is embedded in the membrane structure. The serological specificity of the LPS has been in addition tested by means of monoclonal antiserovar antibodies (Moabs); the results indicated that the LPS structure is endowed of the immunodeterminants of the serovar. The remarkable relevance of this finding for the Leptospira taxonomy is discussed.
Cinco
M
M
Istituto di Microbiologia, University of Trieste, Italy.
Banfi
E
E
Panfili
E
E
D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D002462:Cell Membrane / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D007921:Leptospira interrogans / Q000032:analysis* / Q000145:classification / Q000648:ultrastructure; D008070:Lipopolysaccharides / Q000032:analysis* / Q000276:immunology; D011233:Precipitin Tests
D000911:Antibodies, Monoclonal; D000939:Epitopes; D008070:Lipopolysaccharides