Neutrophil attractant/activation protein-1 (NAP-1) causes human basophil histamine release.
Basophils from five of six human donors released histamine in response to neutrophil attractant/activation protein-1 (NAP-1). Histamine release by this protein was concentration-dependent over the range of 3 x 10(-7) M to 4 x 10(-6) M. At 4 x 10(-6) M, the mean agonist-induced release was 16 +/- 3% (SEM) of total basophil histamine. For the same basophil preparations, release by anti-IgE was 35 +/- 6%. The chemotactic protein did not cause release of histamine from basophils at 0 degrees C or in the presence of 10 mM EDTA. The time-course of histamine release was rapid; release was 43% of maximal after 30 s and maximal after 1 min of incubation. Thus, in addition to its previously characterized neutrophil chemotactic and activating properties, this protein activates human basophils.
White
M V
MV
National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
Yoshimura
T
T
Hook
W
W
Kaliner
M A
MA
Leonard
E J
EJ
D001491:Basophils / Q000187:drug effects* / Q000378:metabolism; D002413:Cations, Divalent / Q000378:metabolism; D002630:Chemotactic Factors / Q000302:isolation & purification / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D006967:Hypersensitivity / Q000097:blood / Q000276:immunology; D016209:Interleukin-8; D007963:Leukocytes, Mononuclear / Q000032:analysis; D013268:Stimulation, Chemical; D013696:Temperature
D002413:Cations, Divalent; D002630:Chemotactic Factors; D016209:Interleukin-8