T-cell receptor V beta 5 usage defines reactivity to a human T-cell receptor monoclonal antibody.
The monoclonal antibody 3D6 reacts with the T-cell receptor (Tcr) on the T-leukemic line HPBALL and with 2-13% of human peripheral blood T lymphocytes. In this study V alpha and V beta expression in a panel of T-cell populations and clones expressing the 3D6 epitope was determined by Southern and northern hybridization analysis. The results demonstrate that these 3D6-positive T cells, regardless of CD4/CD8 phenotype or function, express a gene of the V beta 5 family, also expressed by HPBALL. No correlation was found between 3D6 reactivity and expression of the HPBALL V alpha gene. The results demonstrate that 3D6 recognizes an epitope solely on the Tcr beta chain and that the use of this beta chain, together with an appropriate V alpha, can impart a diverse pattern of reactivity to a T cell.
Lipoldova
M
M
Imperial Cancer Research Fund, St. Bartholomew's Hospital, London, United Kingdom.
Boylston
A W
AW
Yssel
H
H
Owen
M J
MJ
D000911:Antibodies, Monoclonal / Q000276:immunology*; D015139:Blotting, Southern; D000939:Epitopes; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D005796:Genes; D006801:Humans; D011948:Receptors, Antigen, T-Cell / Q000235:genetics* / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D011948:Receptors, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta