Binding of an anti D dimer monoclonal antibody to endotoxin-activated monocytes. Demonstration by immunogold-silver staining.
Blood monocytes exposed to a variety of stimuli, of which endotoxin is a potent one, produce and express on their membrane a procoagulant activity (PCA) which can trigger the formation of pericellular fibrin. We have developed an immunogold silver method using a monoclonal anti-D dimer antibody to detect the presence of crosslinked fibrin in derivatives on the monocyte membrane. The in vitro PCA of endotoxin-stimulated monocyte was shown to correlate significantly with the number of D dimer-positive monocytes. We suggest that this method could be used to identify activated monocytes expressing increased PCA.
Chollet-Martin
S
S
Service d'Immunologie et d'Hématologie Biologiques, CHU X. Bichat, Paris, France.
Ollivier
V
V
Amiral
J
J
de Prost
D
D
D000911:Antibodies, Monoclonal; D001779:Blood Coagulation Factors / Q000276:immunology; D004731:Endotoxins / Q000494:pharmacology; D005338:Fibrin Fibrinogen Degradation Products / Q000276:immunology*; D006046:Gold; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D009000:Monocytes / Q000276:immunology*; D012834:Silver; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D001779:Blood Coagulation Factors; D004731:Endotoxins; D005338:Fibrin Fibrinogen Degradation Products; C036309:fibrin fragment D; C045071:leukocyte procoagulant activity; D012834:Silver; D006046:Gold