Human TCR-gamma+/delta+, CD8+ T lymphocytes recognize tetanus toxoid in an MHC-restricted fashion.
We have analyzed the ability of human gamma+/delta+ T cells to recognize a nominal antigen in association with MHC molecules. A TT-specific T cell line with approximately 40% gamma+/delta+ T cells was established from a hyperimmunized donor, D.F., by stimulation with antigen and autologous APC. Three DF-derived gamma+/delta+ clones were CD8+ as determined by immunofluorescence staining, and by Southern and Northern blotting with probes detecting delta chain rearrangement and delta and gamma chain transcripts, respectively. The gamma+/delta+ clones responded to stimulation with TT, but not TNP-BSA, and autologous APC by proliferation and IFN-gamma production. No proliferation or IFN-gamma production was detected when TT-specific T cell clones were stimulated with either TT or autologous APC only. The response to TT was enhanced by addition of exogenous IL-2. The use of allogeneic APC from 19 donors sharing one HLA-determinant with the autologous donor D.F., showed that the gamma+/delta+ T cells responded to TT with HLA-DR4-related restriction as measured by proliferation and IFN-gamma production. These results demonstrate that gamma/delta receptors can recognize non-MHC-encoded foreign antigen in a self-MHC-restricted fashion.
Kozbor
D
D
Wistar Institute, Philadelphia, Pennsylvania 19104.
Trinchieri
G
G
Monos
D S
DS
Isobe
M
M
Russo
G
G
Haney
J A
JA
Zmijewski
C
C
Croce
C M
CM
D000938:Antigen-Presenting Cells / Q000276:immunology; D000941:Antigens / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D016827:CD8 Antigens; D002460:Cell Line; D000939:Epitopes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006680:HLA Antigens / Q000032:analysis / Q000235:genetics / Q000276:immunology*; D006684:HLA-DR Antigens / Q000276:immunology; D015804:HLA-DR4 Antigen; D006801:Humans; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology*
D000941:Antigens; D000945:Antigens, Differentiation, T-Lymphocyte; D016827:CD8 Antigens; D000939:Epitopes; D006680:HLA Antigens; D006684:HLA-DR Antigens; D015804:HLA-DR4 Antigen; D011948:Receptors, Antigen, T-Cell; D013745:Tetanus Toxoid