Carbonic anhydrase activity in peripheral T-lymphocytes and appearance of the activity during their maturation in the thymus. A histochemical demonstration.
Carbonic anhydrase (CA) activity is demonstrated in lymphoid tissue for the first time using the histochemical (Hansson's) method. A CA-positive reaction was seen in lymphocytes present in T-lymphocyte areas in both the lymph node and the spleen. The most intense staining was seen in the small T-lymphocytes, whereas the medium-sized T-lymphocytes were less markedly stained. The cortical lymphocytes in the thymus were completely devoid of staining, but the small medullary T-lymphocytes stained intensely. The results suggest that peripheral and thymic medullary T-lymphocytes contain CA activity, which appears in these cells during their maturation in the thymus.
Parkkila
S
S
Department of Anatomy, University of Oulu, Finland.
Rajaniemi
H
H
D000818:Animals; D002256:Carbonic Anhydrases / Q000378:metabolism*; D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D006651:Histocytochemistry; D008198:Lymph Nodes / Q000201:enzymology; D008297:Male; D010784:Photomicrography; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000201:enzymology; D013194:Staining and Labeling; D013601:T-Lymphocytes / Q000201:enzymology*; D013950:Thymus Gland / Q000201:enzymology*
D002256:Carbonic Anhydrases