Serological prospects for peptide vaccines against foot-and-mouth disease virus.
Antibodies to a synthetic peptide corresponding to the 141 to 160 amino acid sequence of the protein VP1 of type O foot-and-mouth disease virus (FMDV) neutralize a wider range of type O isolates than anti-virion serum. Extending this peptide at the amino terminus reduced the number of strains neutralized by the antipeptide sera. Reactions with antisera to peptides representing non-contiguous native sequences showed that it was also possible to increase the number of strains effectively neutralized. Selected substitutions of a single amino acid at position 148 markedly altered the neutralizing specificity of antibodies elicited by the 141 to 160 peptide. In particular, a peptide with an L----S substitution at this position induced antibodies which neutralized a type O and a type A virus equally, and guinea-pigs inoculated with it were protected from challenge with either virus. Attempts to isolate variant viruses resistant to neutralization with anti-peptide antibody indicated that these occurred at low frequency, and there was some evidence that resistance may be partially conferred by mutations outside the peptide sequence.
Parry
N R
NR
Department of Virology, Wellcome Biotechnology Ltd, Beckenham, Kent, U.K.
Ouldridge
E J
EJ
Barnett
P V
PV
Clarke
B E
BE
Francis
M J
MJ
Fox
J D
JD
Rowlands
D J
DJ
Brown
F
F
D000595:Amino Acid Sequence; D000818:Animals; D000910:Antibodies, Heterophile / Q000276:immunology; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000138:chemical synthesis / Q000276:immunology*; D005537:Aphthovirus / Q000276:immunology*; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins; D000939:Epitopes; D006168:Guinea Pigs; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D013329:Structure-Activity Relationship; D014612:Vaccines / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
D000910:Antibodies, Heterophile; D000956:Antigens, Viral; D036022:Capsid Proteins; D000939:Epitopes; C442501:VP1 protein, Foot-and-mouth disease virus; D014612:Vaccines; D014614:Vaccines, Synthetic; D014765:Viral Vaccines