The effects of bacterial lipopolysaccharide, anti-receptor antibodies and recombinant interferon on mouse B cell cycle progression using 5-bromo-2'-deoxyuridine/Hoechst 33258 dye flow cytometry.
Polyclonal stimulation of resting B cells with anti-antigen receptor antibodies [anti-IgM mu chain antibody (anti-mu)] or with bacterial lipopolysaccharide (LPS) stimulates a proportion of B cells to proliferate. Exposure of resting B cells to both LPS and anti-mu activates a larger population of resting B cells than either alone, suggesting a synergistic effect of these two stimuli. Although recombinant interferon (rIFN) either alone or in combination with anti-mu has no apparent proliferative activity (as measured by [3H]thymidine incorporation), application of 5-bromo-2'-deoxyuridine/Hoechst 33258 dye flow cytometry reveals a distinct effect of rIFN on B cell growth. In the presence of anti-mu plus LPS, rIFN causes the majority of B cells to enter the cell cycle (CC), but a subset of B cells remains in the resting stage. Another subset of B cells has extremely rapid CC transit times, with a CC duration of less than 10 h. These studies show that both anti-mu and LPS are competence factors (which move cells from the G0 phase to the G1 phase). LPS acts also as a CC progression factor, while rIFN is a CC potentiating factor.
Seyschab
H
H
Department of Human Genetics, University of Würzburg, FRG.
Friedl
R
R
Schindler
D
D
Hoehn
H
H
Rabinovitch
P S
PS
Chen
U
U
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000187:drug effects; D001973:Bromodeoxyuridine; D002453:Cell Cycle / Q000187:drug effects; D005434:Flow Cytometry / Q000379:methods; D007148:Immunoglobulin mu-Chains / Q000502:physiology; D007158:Immunologic Techniques; D007372:Interferons / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008213:Lymphocyte Activation; D051379:Mice; D011947:Receptors, Antigen, B-Cell / Q000502:physiology*; D011994:Recombinant Proteins
D007148:Immunoglobulin mu-Chains; D008070:Lipopolysaccharides; D011947:Receptors, Antigen, B-Cell; D011994:Recombinant Proteins; D007372:Interferons; D001973:Bromodeoxyuridine