Activation of CD4 cells by fibronectin and anti-CD3 antibody. A synergistic effect mediated by the VLA-5 fibronectin receptor complex.
In this study, fibronectin synergized with anti-CD3 antibody to promote CD4 cell proliferation in a serum-free culture system. The cell-adhesive domain plus additional regions of the fibronectin molecule are involved in this synergy. Anti4B4(CDw29) antibody blocked the activation of CD4 cells in this system. Furthermore, it is the VLA-5 protein within the set of molecules recognized by anti-4B4 that serves as a fibronectin receptor on the CD4 lymphocytes. The VLA-5 fibronectin receptor was mainly expressed on CD4+ CD45R-CDw29+ cells and may in part contribute to the unique function of these cells.
Matsuyama
T
T
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115.
Yamada
A
A
Kay
J
J
Yamada
K M
KM
Akiyama
S K
SK
Schlossman
S F
SF
Morimoto
C
C
D000911:Antibodies, Monoclonal / Q000276:immunology; D000943:Antigens, Differentiation / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology*; D017252:CD3 Complex; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D005109:Extracellular Matrix / Q000502:physiology; D005353:Fibronectins / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D019012:Integrin beta1; D008213:Lymphocyte Activation; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D016024:Receptors, Cytoadhesin / Q000502:physiology*; D016029:Receptors, Very Late Antigen / Q000502:physiology*
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D000945:Antigens, Differentiation, T-Lymphocyte; D017252:CD3 Complex; D005353:Fibronectins; D019012:Integrin beta1; D011948:Receptors, Antigen, T-Cell; D016024:Receptors, Cytoadhesin; D016029:Receptors, Very Late Antigen