An insoluble chromolytic substrate for the determination of proteolytic activity.
A new insoluble chromolytic substrate for the determination of proteolytic activity was prepared by immobilization of dyed casein into the structure of polyacrylamide gel. The prepared substrate could detect approximately 0.1 microgram of trypsin per ml. The spontaneous leakage of dyed casein molecules in water solutions was negligible.
Safarík
I
I
Laboratory of Analytical Chemistry, South Bohemian Biological Centre, Ceské Budĕjovice, Czechoslovakia.
D000180:Acrylic Resins; D002364:Caseins; D004800:Enzymes, Immobilized; D007103:Immobilization; D007202:Indicators and Reagents; D010447:Peptide Hydrolases / Q000378:metabolism*; D013194:Staining and Labeling
D000180:Acrylic Resins; D002364:Caseins; D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; C016680:polyacrylamide gels; D010447:Peptide Hydrolases