Human immunodeficiency virus 1 tat protein binds trans-activation-responsive region (TAR) RNA in vitro.
tat, the trans-activator protein for human immunodeficiency virus 1 (HIV-1), has been expressed in Escherichia coli from synthetic genes. Purified tat binds specifically to HIV-1 trans-activation-responsive region (TAR) RNA in gel-retardation, filter-binding, and immunoprecipitation assays. tat does not bind detectably to antisense TAR RNA sequences, cellular mRNA sequences, variant TAR RNA sequences with altered stem-loop structures, or TAR DNA.
Dingwall
C
C
Medical Research Council Laboratory of Molecular Biology, Cambridge, England.
Ernberg
I
I
Gait
M J
MJ
Green
S M
SM
Heaphy
S
S
Karn
J
J
Lowe
A D
AD
Singh
M
M
Skinner
M A
MA
Valerio
R
R
D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D015696:Gene Products, tat; D005796:Genes; D005813:Genes, Synthetic; D005814:Genes, Viral; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009856:Oncogene Proteins, Viral / Q000378:metabolism; D010957:Plasmids; D011485:Protein Binding; D012313:RNA / Q000378:metabolism*; D016372:RNA, Antisense; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D014158:Transcription, Genetic; D054322:tat Gene Products, Human Immunodeficiency Virus
D015696:Gene Products, tat; D009856:Oncogene Proteins, Viral; D016372:RNA, Antisense; D011994:Recombinant Proteins; D014157:Transcription Factors; D054322:tat Gene Products, Human Immunodeficiency Virus; D012313:RNA