Impact of the genetic background of transgenic mice upon the formation and timing of choroid plexus papillomas.
Transgenic mice harboring the SV40 large T antigen gene in a C57B1/6J genetic background (SV11) first express this gene at 1-2 weeks of age, develop papillomas of the choroid plexus by 80-90 days, and die within 125 days after birth. Transgenic mice harboring the same transgene in a (SV11-C57Bl/6J x NZW/lacJ) F1 genetic background express considerably lower levels of the transgene mRNA at comparable times after birth. As a consequence, tumor development and death are delayed. The NZW mice appear to contribute a dominant negative regulator for the expression of the SV40 large T antigen transgene, which in turn has a dramatic effect upon the time of appearance of tumors and the death of these transgenic animals.
Cho
H J
HJ
Department of Biology, Princeton University, New Jersey.
Seiberg
M
M
Georgoff
I
I
Teresky
A K
AK
Marks
J R
JR
Levine
A J
AJ
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics*; D002551:Cerebral Ventricle Neoplasms / Q000235:genetics* / Q000382:microbiology; D002831:Choroid Plexus; D051379:Mice; D008822:Mice, Transgenic; D010212:Papilloma / Q000235:genetics* / Q000382:microbiology; D010641:Phenotype; D012313:RNA / Q000302:isolation & purification; D015347:RNA Probes; D012367:RNA, Viral / Q000302:isolation & purification; D013539:Simian virus 40 / Q000235:genetics; D013997:Time Factors
D000914:Antibodies, Viral; D000952:Antigens, Polyomavirus Transforming; D015347:RNA Probes; D012367:RNA, Viral; D012313:RNA