Alpha-factor-directed synthesis and secretion of beta-subunit of human choriogonadotropin (beta-hCG) in yeast S. cerevisiae.
A couple of shuttle vectors (pYNA and pYNB), containing a sequence coding for the leader region of the alpha-factor precursor (alpha-factor leader sequence) and the origin of 2 micron circle, were constructed. The cDNA for the beta-hCG (145aa) was fused in phase to the 3'-end of the alpha-factor leader sequence and transformed into the yeast Saccharomyces cerevisiae. The yeast cells carrying this hybrid gene synthesized and secreted a protein with the immunoactivity of beta-hCG in an amount of 45 micrograms per liter of culture. The preliminary characterization of this protein is also reported in this paper.
Tang
Y
Y
Shanghai Institute of Cell Biology, Academia Sinica.
Kuang
D R
DR
Wu
R
R
D000595:Amino Acid Sequence; D006063:Chorionic Gonadotropin / Q000096:biosynthesis* / Q000235:genetics; D018997:Chorionic Gonadotropin, beta Subunit, Human; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D010446:Peptide Fragments / Q000096:biosynthesis* / Q000235:genetics; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
D006063:Chorionic Gonadotropin; D018997:Chorionic Gonadotropin, beta Subunit, Human; D010446:Peptide Fragments