Demonstration of in vitro synthesis of human papilloma viral proteins from hand and foot warts.
HPV particles purified from [35S]-methionine labeled and unlabeled halves of single hand and foot warts have been fractionated into empty, light full, and heavy full particles by buoyant density gradient centrifugation, and their proteins analyzed by two-dimensional gel electrophoresis (IEF and NEPHGE) and visualized by either fluorography or silver staining. The L1 coat protein (54 Kd) was found in trace amounts in unmodified and slightly modified forms in the labeled empty and light full particles but could not be detected in the labeled heavy particles. L1 appeared to exist in the three unlabeled particle types in differentially modified forms. A putative L2 protein was also found to be modified (74-80 Kd) and was found preferentially in the unlabeled heavy full particles. The commercial cross-reactive BPV antibody recognized a labeled 58-Kd protein found predominantly in the empty and light full particles and a pair of proteins (41-42 Kd) found unlabeled in the heavy full particles. Besides L1, there were several other proteins (IEF 40 Kd; NEPHGE 42, 38, and 36 Kd) which were detected labeled in the empty particles and in increasing unlabeled amounts in the light full and heavy full particles. Four proteins (IEF 66, 13 and 11 Kd, and NEPHGE 9 Kd) were found exclusively in the full particles and may be involved in packing the viral genome. These observations suggest that a virus particle assembly pathway exists from the empty particles, via the light full, to the mature heavy full particles.
Fey
S J
SJ
Institute of Human Genetics, Aarhus University, Denmark.
Nielsen
T
T
Vetner
M
M
Storgaard
L
L
Smed
V
V
Larsen
P M
PM
D052688:Bovine papillomavirus 1 / Q000032:analysis; D002213:Capsid / Q000032:analysis / Q000096:biosynthesis* / Q000648:ultrastructure; D015180:Electrophoresis, Gel, Two-Dimensional; D005533:Foot Dermatoses / Q000378:metabolism*; D006229:Hand Dermatoses / Q000378:metabolism*; D006801:Humans; D007525:Isoelectric Focusing; D008854:Microscopy, Electron; D008970:Molecular Weight; D027383:Papillomaviridae / Q000032:analysis*; D011233:Precipitin Tests; D013194:Staining and Labeling; D014860:Warts / Q000378:metabolism*