Ultrastructure and some biological properties of influenza A virus. III. The role of influenza A virus lipids in interferon induction.
Experiments were carried out with A/USSR/053/74/H3N2 influenza virus. To remove lipids from virus particles, the enzyme phospholipase C, butanol-ether and the detergent cytylpyridinum chloride were used. Mild treatment of the influenza virus with phospholipase C resulted in a slight decrease in the activity of both surface antigens of the virions as well as in a suppression of their infectivity. Such virus is capable of IFN induction in mouse. Long-term (25 h) treatment of the virus with phospholipase C caused gross destruction of virions. Thus, despite the preserved neuraminidase activity the preparation was no longer capable of IFN induction. The treatment with butanol-ether and the detergent cytylpyridinum chloride brought similar results--fully preserved hemagglutination and neuraminidase activity and a complete loss of IFN induction.
Chomik
M
M
Department of Immunology of Infectious Diseases, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.
D000956:Antigens, Viral; D006388:Hemagglutinins / Q000032:analysis; D009980:Influenza A virus / Q000472:pathogenicity / Q000648:ultrastructure*; D007372:Interferons / Q000096:biosynthesis*; D008055:Lipids / Q000494:pharmacology*; D009439:Neuraminidase / Q000032:analysis; D010738:Type C Phospholipases / Q000494:pharmacology
D000956:Antigens, Viral; D006388:Hemagglutinins; D008055:Lipids; D007372:Interferons; D010738:Type C Phospholipases; D009439:Neuraminidase