Complementary addressed modification of nucleic acids with the alkylating derivatives of oligothymidylate ethyl phosphotriesters. Effect of the phosphotriester fragments' configuration.
The alkylating derivatives of four individual diastereomers of the oligonucleotide [dTp(Et)]3dTpU and two individual diastereomers of oligonucleotide [dTp(Et)dTp]4 have been synthesized. The reagents with the phosphorus atoms in the enantiomeric p" configuration are shown to be more efficient in reacting with poly(dA) and with nucleic acids in Krebs-2 ascites carcinoma cells compared to those with the phosphorus atoms in the p' configuration.
Abramova
T V
TV
Institute of Bioorganic Chemistry, Siberian Division, USSR Academy of Sciences, Novosibirsk.
Vlassov
V V
VV
Lebedev
A V
AV
Ryte
A S
AS
D000478:Alkylation; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D009838:Oligodeoxyribonucleotides / Q000378:metabolism*; D010755:Organophosphates / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D009838:Oligodeoxyribonucleotides; D010755:Organophosphates; D009943:Organophosphorus Compounds; D012313:RNA; D004247:DNA