Co-expression of Mac-1 and p150,95 on CD5+ B cells. Structural and functional characterization in a human chronic lymphocytic leukemia.
The leukocyte adhesion receptors (LAR) Mac-1 and p150,95 were thought to occur only on myeloid cells, but recently, mouse Ly-1+ (CD5+) B and pro-B cells were shown to bind M1/70 monoclonal antibody, an anti-Mac-1 antibody. Using immunoprecipitation, sodium dodecyl sulfate gel electrophoresis, immunofluorescence and flow cytometry, we have studied the expression of LAR in human CD5+ B cells from chronic lymphocytic leukemia (B-CLL) patients. 170,95 kDa Mac-1 and p150,95, as well as 180,95 kDa LFA-1 heterodimers are shown to be co-expressed on CD5+ mu + kappa + B cells from a patient where they retain their known function in homotypic cell-cell adhesion. Therefore, functional Mac-1 and p150,95 LAR are not restricted to myeloid cells.
De la Hera
A
A
Basel Institute for Immunology, Switzerland.
Alvarez-Mon
M
M
Sanchez-Madrid
F
F
Martinez
C
C
Durantez
A
A
D000911:Antibodies, Monoclonal / Q000276:immunology; D000943:Antigens, Differentiation / Q000502:physiology*; D018956:CD5 Antigens; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006801:Humans; D016167:Integrin alphaXbeta2; D007945:Leukemia, Lymphoid / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D016177:Macrophage-1 Antigen; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D018956:CD5 Antigens; D016167:Integrin alphaXbeta2; D016177:Macrophage-1 Antigen