Purification and reconstitution into liposomes of an integral membrane protein conferring immunity to colicin A.
The immunity protein to colicin A protects producing cells from the action of this pore-forming toxin. It is located into the cytoplasmic membrane. This protein has been 'tagged' with an epitope from the colicin A protein for which a monoclonal antibody is available. The fusion protein (named VL1) has been purified after extraction from the membrane in two steps using a chromatofocusing and an immunoadsorbant chromatography. The purified protein has then been reconstituted into lipid vesicles.
Geli
V
V
Centre de Biochimie et de Biologie Moléculaire du C.N.R.S., Marseille, France.
Knibiehler
M
M
Bernadac
A
A
Lazdunski
C
C
D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000378:metabolism; D002846:Chromatography, Affinity / Q000379:methods; D003087:Colicins; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D015151:Immunoblotting; D008081:Liposomes / Q000378:metabolism*; D008565:Membrane Proteins / Q000302:isolation & purification* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000302:isolation & purification
D001426:Bacterial Proteins; D003087:Colicins; D000939:Epitopes; D008081:Liposomes; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins; C007104:colicin immunity proteins