Novel redistribution of myosin-containing filaments in cultured keratinocytes identified by a human monoclonal autoantibody.
A time-dependent redistribution of microfilaments was observed in cultured human keratinocytes using a human monoclonal autoantibody specific for myosin. Immunofluorescent staining revealed that 5 days after plating keratinocytes in either 0.1 mM or 2.0 mM Ca++, myosin was distributed uniformly throughout the cytoplasm. At day 6, parallel arrays of myosin-containing microfilaments were prominent in the cell peripheries. At day 7 the microfilaments formed circumferential rings. The distribution of the microfilaments was disrupted by cytochalasin but not by colchicine, indicating that this novel distribution of myosin was not dependent on colchicine-sensitive vimentin intermediate filaments. The time-dependent redistribution of myosin was not influenced by cell population density, cell shape or cell cycle phase, except for mitotic cells in which myosin was distributed diffusely through the cytoplasm. If, as suggested by Kolega (9), microfilaments align parallel to the direction of applied tension, the redistribution of myosin-containing microfilaments in cultured keratinocytes may reflect the increased tension between cells resulting from increasing strength of cell-cell junctions over time. In sectioned human skin, myosin was localized in the peripheral cytoplasm of stratified epidermal cells. Tensions arising from the numerous desmosomal junctions between cells in vivo could account for this distribution of myosin.
Williams
C L
CL
Department of Immunology, Mayo Clinic and Foundation, Rochester, Minnesota 55905.
Lennon
V A
VA
Pittelkow
M R
MR
D008841:Actin Cytoskeleton / Q000032:analysis / Q000378:metabolism* / Q000648:ultrastructure; D001323:Autoantibodies / Q000378:metabolism; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000032:analysis / Q000378:metabolism* / Q000648:ultrastructure; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism / Q000648:ultrastructure; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D007365:Intercellular Junctions / Q000648:ultrastructure; D007633:Keratins; D008297:Male; D009218:Myosins / Q000032:analysis / Q000378:metabolism*; D013997:Time Factors
D001323:Autoantibodies; D007633:Keratins; D009218:Myosins