title: [A photographic microfluorimetric method for determining the DNA content in individual human chromosomes].
abstract: A method for determination of DNA contents in individual human chromosomes has been elaborated based of the two step analysis: 1) identification of chromosomes by Q-banding; 2) photographic microfluorimetry of chromosomes after the Feulgen staining (a fluorescent variant using a Schiff-type reagent of Auramine-SO2). The DNA content in 24 human chromosomes was calculated in absolute values (fg). The data obtained are compared with the evidence published elsewhere and provided by different cytophotometric methods.
authors: T R
authors: TR
authors: Sukhikh
authors: A M
authors: AM
authors: Kalimagambetov
authors: I Ia
authors: IIa
authors: Barskiĭ
authors: S A
authors: SA
authors: Khomenkova
authors: A E
authors: AE
authors: Vinogradov
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002871:Chromosome Banding / Q000379:methods; D002877:Chromosomes, Human / Q000032:analysis*; D004396:Coloring Agents; D003592:Cytophotometry / Q000379:methods*; D004247:DNA / Q000032:analysis*; D006801:Humans; D008214:Lymphocytes / Q000032:analysis; D008677:Metaphase; D010781:Photography / Q000379:methods*; D012394:Rosaniline Dyes; D013194:Staining and Labeling / Q000379:methods
chemical_list: D004396:Coloring Agents; C007722:Feulgen stain; D012394:Rosaniline Dyes; D004247:DNA
vernacular_title: Fotograficheskiĭ mikrofluorimetricheskiĭ metod opredeleniia soderzhaniia DNK v individual'nykh khromosomakh cheloveka.