title: Monoclonal antibody to galactosylceramide: discrimination of structural difference in the ceramide moiety.
abstract: A mouse monoclonal antibody (mAb) was developed against monohexaosylceramide. This mAb differentially reacted on thin-layer chromatograms with 3 types of galactosylceramide (GalCer) obtained from bovine brain. Structural analysis of the 3 glycolipids revealed that they consisted of the same galactose and sphingosine but of apparently different fatty acids. Among the GalCers, the mAb reacted with teh two GalCers which contained alpha-hydroxy fatty acids, but not with GalCer composed of nonhydroxy fatty acids. These findings suggest that not only that the mAb discriminated the fatty acid composition in the ceramide moiety of GalCer, but also that the ceramide structure defines the immunological epitope as it is known to do for the carbohydrate moiety of glycosphingolipid.
authors: Second Department of Internal Medicine, Kumamoto University Medical School, Japan.
authors: H
authors: H
authors: Nakakuma
authors: M
authors: M
authors: Arai
authors: T
authors: T
authors: Kawaguchi
authors: K
authors: K
authors: Horikawa
authors: M
authors: M
authors: Hidaka
authors: K
authors: K
authors: Sakamoto
authors: M
authors: M
authors: Iwamori
authors: Y
authors: Y
authors: Nagai
authors: K
authors: K
authors: Takatsuki
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex / Q000032:analysis; D001923:Brain Chemistry; D002241:Carbohydrates / Q000032:analysis; D002518:Ceramides / Q000276:immunology*; D002554:Cerebrosides / Q000276:immunology*; D002855:Chromatography, Thin Layer; D000939:Epitopes / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005699:Galactosylceramides / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D010190:Pancreatic Neoplasms / Q000276:immunology; D051381:Rats; D014407:Tumor Cells, Cultured / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D002241:Carbohydrates; D002518:Ceramides; D002554:Cerebrosides; D000939:Epitopes; D005227:Fatty Acids; D005699:Galactosylceramides