Activity of a cloned Xenopus albumin gene promoter in the homologous frog oocyte system.
The activity of the liver-specific promoter from the Xenopus laevis 68 kDa albumin gene in homologous oocytes has been analysed by microinjection. We find that the albumin promoter functions relatively efficiently in oocytes, directing the synthesis of correctly initiated transcripts, and deletion analysis reveals that only a small amount of upstream sequence is required for full activity.
Old
R W
RW
Department of Biological Sciences, University of Warwick, Coventry, U.K.
Sweeney
G E
GE
Brooks
A R
AR
D000818:Animals; D003001:Cloning, Molecular; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D008099:Liver / Q000032:analysis; D008297:Male; D009865:Oocytes / Q000032:analysis / Q000378:metabolism*; D011061:Poly A / Q000032:analysis; D011401:Promoter Regions, Genetic; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012709:Serum Albumin / Q000235:genetics*; D013629:Tamoxifen / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects; D014170:Transformation, Genetic; D014284:Triiodothyronine / Q000494:pharmacology; D014982:Xenopus laevis
D012333:RNA, Messenger; D012709:Serum Albumin; D014284:Triiodothyronine; D013629:Tamoxifen; D011061:Poly A; D012313:RNA; D003907:Dexamethasone