Biochemical and immunochemical characterization of surface and excretory-secretory antigens of Loa loa microfilariae.
Detergent solubilized extracts of 125Iodogen surface-labelled Loa loa microfilariae revealed a relatively simple profile of two strongly labelled molecules of 23 and 67 kDa for blood microfilariae and several strongly labelled molecules of 23, 40, 42-67 kDa for in vitro born microfilariae. In addition, there were other weakly labelled molecules which were resolved after prolonged autoradiographic exposure. Surface molecules of 28, 29, and 33 kDa were unique to blood microfilariae, a 14.4 kDa molecule was unique to in vitro born microfilariae and molecules of 23, 40, and 75-84 kDa were common to both forms of microfilariae. The profile of excretory-secretory products consisted of molecules of 14.4-198 kDa. Human albumin was a predominant component of surface molecules and excretory-secretory products from blood microfilariae. Immunoprecipitation with occult and microfilaremic loaiasis sera demonstrated that the 23 kDa surface molecule and excretory-secretory products of 14.4 and 33 kDa were only recognized by occult loaiasis sera whereas surface molecules of 40 and 75-84 kDa and excretory-secretory products of 28 and 67 kDa were recognised by both sera. Studies with heterologous sera demonstrated that with the exception of the 75-84 kDa antigens, all the L. loa microfilarial surface antigens contained epitopes which were restricted to filarial parasites. Further studies revealed that the 23 kDa antigen was a protein which contained neither asparagine-N-linked oligosaccharides nor interchain disulfide-linkages.
Egwang
T G
TG
International Centre for Medical Research (CIRMF), Franceville, Gabon.
Dupont
A
A
Akué
J P
JP
Pinder
M
M
D000818:Animals; D000947:Antigens, Helminth / Q000302:isolation & purification*; D000954:Antigens, Surface / Q000302:isolation & purification*; D000939:Epitopes / Q000032:analysis; D006801:Humans; D066298:In Vitro Techniques; D008117:Loa / Q000276:immunology*; D008842:Microfilariae / Q000276:immunology; D011233:Precipitin Tests; D012709:Serum Albumin / Q000032:analysis
D000947:Antigens, Helminth; D000954:Antigens, Surface; D000939:Epitopes; D012709:Serum Albumin