Human and mouse cementum proteins immunologically related to enamel proteins.
SDS-polyacrylamide gel electrophoresis, immunoblot and amino acid composition analyses were applied to human and mouse acellular cementum proteins immunologically related to enamelins and amelogenins. In this analysis, anti-mouse amelogenin, anti-human enamelin and synthetic peptide (e.g., -LPPHPGHPGYIC-) antibodies were shown to cross-react with tooth crown-derived enamelin with a molecular mass of 72,000 Da (72 kDa), amelogenins (26 kDa), and also to four human cementum proteins (72, 58, 50 and 26 kDa) and two mouse cementum proteins (72 and 26 kDa). Each of the antibodies recognized tooth root-derived cementum polypeptides which share one or more epitopes with tooth crown-derived enamel proteins. The molecular mass and isoelectric points for crown-derived and root-derived enamel-related proteins were similar. Analysis of human and mouse cementum proteins revealed a characteristic amino acid composition enriched in glutamyl, serine, glycine, alanine, proline, valine and leucine residues; compared to the major enamel protein amelogenin, cementum proteins were low in proline, histidine and methionine. The human and mouse putative intermediate cementum proteins appear to represent a distinct class of enamel-related proteins. Moreover, these results support the hypothesis that epithelial root sheath epithelia express several cementum proteins immunologically related to canonical enamel proteins.
Slavkin
H C
HC
Department of Basic Sciences (Biochemistry), School of Dentistry, University of Southern California, Los Angeles 90089-0191.
Bessem
C
C
Fincham
A G
AG
Bringas
P
P
Santos
V
V
Snead
M L
ML
Zeichner-David
M
M
D053523:Amelogenin; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D003739:Dental Cementum / Q000032:analysis* / Q000276:immunology; D003746:Dental Enamel Proteins / Q000032:analysis* / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D000939:Epitopes / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D011506:Proteins / Q000032:analysis* / Q000276:immunology; D012995:Solubility
D053523:Amelogenin; C507200:Amelx protein, mouse; D000596:Amino Acids; D003746:Dental Enamel Proteins; D000939:Epitopes; D011506:Proteins; C038456:tuftelin