Antigen-specific helper function of cell-free T cell products bearing TCR V beta 8 determinants.
Although the T cell receptor (TCR) alpha beta heterodimer and its encoding genes have been characterized, a cell-free form of this receptor, which is needed for the study of functional or ligand-binding properties of the receptor, has not previously been isolated. When the cell-free supernatant products of activated cloned T helper (TH) cells were found to mediate helper activity with antigen specificity identical to that of intact T cells, experiments were carried out to determine whether this functional activity was mediated by a cell-free form of TCR-related material. A disulfide-linked dimer indistinguishable from the T cell surface alpha beta heterodimer was precipitated from cell-free supernatants of cloned TH cells with F23.1, a monoclonal antibody specific for a TCR V beta 8 determinant. Moreover, when cell-free TH products were bound to and eluted from immobilized F23.1, these affinity-purified materials had antigen-specific and major histocompatibility complex-restricted helper activity that synergized with recombinant lymphokines in the generation of B cell antibody responses. These findings suggest that the factor isolated from T cell supernatants is a cell-free form of the TCR alpha beta dimer.
Guy
R
R
Experimental Immunology Branch, National Cancer Institute, Bethesda, MD 20892.
Ullrich
S J
SJ
Foo-Philips
M
M
Hathcock
K S
KS
Appella
E
E
Hodes
R J
RJ
D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D004220:Disulfides; D000939:Epitopes / Q000276:immunology; D006241:Haptens; D006433:Hemocyanins / Q000276:immunology; D006649:Histocompatibility Antigens / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007163:Immunosorbent Techniques; D007376:Interleukin-2 / Q000494:pharmacology; D015847:Interleukin-4; D007378:Interleukins / Q000494:pharmacology; D008213:Lymphocyte Activation; D046911:Macromolecular Substances; D051379:Mice; D008970:Molecular Weight; D011948:Receptors, Antigen, T-Cell / Q000276:immunology* / Q000302:isolation & purification; D011994:Recombinant Proteins; D012710:Serum Albumin, Bovine / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D014301:Trinitrobenzenes / Q000276:immunology
D000911:Antibodies, Monoclonal; D000941:Antigens; D004220:Disulfides; D000939:Epitopes; D006241:Haptens; D006649:Histocompatibility Antigens; D007074:Immunoglobulin G; D007376:Interleukin-2; D007378:Interleukins; D046911:Macromolecular Substances; D011948:Receptors, Antigen, T-Cell; D011994:Recombinant Proteins; D014301:Trinitrobenzenes; C054401:trinitrophenyl keyhole limpet hemocyanin; C038601:trinitrophenyl-bovine serum albumin; D015847:Interleukin-4; D012710:Serum Albumin, Bovine; D006433:Hemocyanins