title: Expression of synthetic genes encoding bovine and human basic fibroblast growth factors (bFGFs) in Escherichia coli.
abstract: Synthetic genes encoding bovine and human basic fibroblast growth factors (bFGFs) were assembled and cloned using established Escherichia coli expression plasmids. Transformed E. coli cells were able to synthesize either a fusion protein, comprising the first seven amino acids of beta-galactosidase, a linker fragment and bovine FGF, or genomic human bFGF. The two growth factors were purified from E. coli lysates by cation exchange and heparin-Sepharose affinity chromatography. The purified recombinant proteins were biologically active as monitored by their mitogenic activity for bovine aortic endothelial cells and their angiogenic capacity in the rabbit cornea.
authors: PROGEN Biotechnik GmbH, Heidelberg, F.R.G.
authors: W
authors: W
authors: Knoerzer
authors: H P
authors: HP
authors: Binder
authors: K
authors: K
authors: Schneider
authors: P
authors: P
authors: Gruss
authors: J E
authors: JE
authors: McCarthy
authors: W
authors: W
authors: Risau
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D001483:Base Sequence; D015153:Blotting, Western; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D003001:Cloning, Molecular; D003315:Cornea / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D005346:Fibroblast Growth Factors / Q000235:genetics* / Q000302:isolation & purification; D005786:Gene Expression Regulation; D005813:Genes, Synthetic; D005822:Genetic Vectors; D006801:Humans; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000302:isolation & purification / Q000378:metabolism; D010766:Phosphorylation; D010957:Plasmids; D011817:Rabbits; D011994:Recombinant Proteins / Q000302:isolation & purification; D013194:Staining and Labeling
chemical_list: D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; D005346:Fibroblast Growth Factors