title: Blockade of the inotropic effect of Bay K 8644 by cytochalasin-B and phloretin.
abstract: 1. The positive inotropic effect in rabbit atria and papillary muscles of Bay K 8644 is blocked by cytochalasin-B (Cyto-B) and phloretin, two compounds known to block the facilitated diffusion of glucose. These compounds do not change the concentration-response curve of calcium. 2. Cyto-B is more potent in atria than in papillary muscles, 10(-7) M having a maximal effect in atria whereas 2 x 10(-5) M was required for a maximal effect in papillary muscles. Phloretin was fully effective at 10(-4) M, the only concentration tested. 3. The inotropic effect of Bay K 8644 was virtually abolished in atria bathed in a glucose-free medium or one containing 5 mM pyruvate. The contractile response to Bay K 8644 of papillary muscles was not changed significantly in glucose-free or in pyruvate-containing medium. 4. Cyto-B (2 x 10(-5) M) caused a slight but significant increase in the KD for the binding of nitrendipine to a crude sarcolemnal preparation from rabbit ventricles. The Bmax was unchanged. 5. These results may best be explained by the hypothesis that there is a metabolic requirement for the inotropic effect of Bay K 8644.
authors: Department of Pharmacology, Faculty of Medicine, Dalhousie University, Halifax, N.S., Canada.
authors: P E
authors: PE
authors: Dresel
authors: A
authors: A
authors: Ogbaghebriel
mesh_terms: D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D000818:Animals; D003571:Cytochalasin B / Q000494:pharmacology*; D005260:Female; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects*; D010693:Phloretin / Q000494:pharmacology*; D011817:Rabbits; D013268:Stimulation, Chemical
chemical_list: D003571:Cytochalasin B; D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; D010693:Phloretin