[Isolation and partial characterization of poly-(a)+mRNA of soybean grown in the presence of gibberellic acid].
Poly(A)+mRNA was prepared by chromatography on the oligo (dT)-cellulose column from total RNA of epicotyls of soybean grown on the medium with gibberellic acid. Poly(A)+mRNA was prepared by the same method from control plants. Total RNA, poly-A and poly(A)+mRNAs were analyzed by electrophoresis on 1.5% agarose gel under denaturing conditions. The poly(A)+mRNA distribution was shown to be of polydispersional character, cDNA was synthesized by reverse transcription. Basing on cDNA-poly(A)+mRNA hybridization a conclusion is drawn that gibberellic acid may activate new types of mRNAs and, probably, increases the synthesis of some poly(A)+mRNA populations.
Dzhokhadze
D I
DI
Mamulashvili
N A
NA
Lomidze
N V
NV
Nazarova
L A
LA
D003470:Culture Media; D004247:DNA / Q000235:genetics; D005875:Gibberellins / Q000494:pharmacology*; D009693:Nucleic Acid Hybridization; D010937:Plant Growth Regulators / Q000494:pharmacology*; D011061:Poly A / Q000032:analysis / Q000302:isolation & purification*; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis / Q000302:isolation & purification*; D013025:Glycine max / Q000032:analysis* / Q000187:drug effects / Q000235:genetics
D003470:Culture Media; D005875:Gibberellins; D010937:Plant Growth Regulators; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA; C007842:gibberellic acid
Vydelenie i chastichnaia kharakteristika poli-(A)+mRNK soi, vyrashchennoĭ v prisutstvii gibberellovoĭ kisloty.