title: Electrochemical analysis of hypothalamic serotonin metabolism accompanied by immobilization stress in rats.
abstract: The effects of stress on serotonin metabolism in the lateral hypothalamic area (LHA) were investigated. Differential pulse voltammetry with a carbon fiber electrode was used to measure 5-hydroxyindoleacetic acid (5-HIAA), the metabolic product of the serotonin (5-HT). High-performance liquid chromatography with electrochemical detection was also used to analyze these compounds in the cerebrospinal fluid (CSF). The peak in voltammogram at an oxidation potential of +230 mV (P3) was identified as 5-HIAA by pharmacological manipulations that are known to affect 5-HT metabolism. A significant increase in 5-HIAA concentration in the LHA was detected after immobilization. An intraperitoneal injection of 5-hydroxytryptophan (5-HTP; a precursor of 5-HT) increased the height of P3, and injection of pargyline prevented the effect of 5-HTP during the course of increasing P3. These results support our previous conclusion that immobilization-induced anorexia might be mediated through activation of serotonergic mechanisms in the LHA.
authors: Department of Physiology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
authors: N
authors: N
authors: Shimizu
authors: Y
authors: Y
authors: Oomura
authors: K
authors: K
authors: Aoyagi
mesh_terms: D015102:3,4-Dihydroxyphenylacetic Acid / Q000032:analysis / Q000134:cerebrospinal fluid; D006916:5-Hydroxytryptophan / Q000494:pharmacology; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry / Q000379:methods; D006897:Hydroxyindoleacetic Acid / Q000032:analysis* / Q000134:cerebrospinal fluid; D007026:Hypothalamic Area, Lateral / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010293:Pargyline / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D012149:Restraint, Physical; D012701:Serotonin / Q000378:metabolism*; D013312:Stress, Physiological / Q000378:metabolism*; D013997:Time Factors
chemical_list: D015102:3,4-Dihydroxyphenylacetic Acid; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D010293:Pargyline; D006916:5-Hydroxytryptophan