A comparative study of PAS-phosphotungstic acid-Diamine Supra Blue FGL and immunological reactions for type I collagen.
Immunohistochemical techniques proved valuable in histological studies of various types of collagens. However drawbacks include non-specific reactions of antibodies, masking of antigens, and the high cost of antibodies. This study was undertaken to ascertain the specificity of the PAS-phosphotungstic acid-Diamine Supra Blue FGL (PAS-PTA-DSB-FGL) reaction for type I collagen, differentiating it from other collagens. Duplicate series of methacarn-fixed sections of various tissues were treated with the PAS-PTA-DSB FGL reaction and the peroxidase-antiperoxidase (PAP) technique for type I collagen and the staining patterns were compared. Fibers binding the blue dye were found only at sites reacting with antibodies against type I collagen. These observations indicate that the PAS-PTA-DSB FGL procedure is suitable for visualization of type I collagen, e.g. in screening of large series of sections and in the practice of surgical and autopsy pathology.
Rao
R N
RN
Department of Pathology, Medical College of Georgia, Augusta 30912-3605.
Fallman
P M
PM
Falls
D G
DG
Meloan
S N
SN
D001980:Bronchi / Q000378:metabolism; D003094:Collagen / Q000378:metabolism*; D005404:Fixatives; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D013154:Spleen / Q000378:metabolism; D013194:Staining and Labeling
D005404:Fixatives; D003094:Collagen