Ultrastructural localisation of substance P and choline acetyltransferase in endothelial cells of rat coronary artery and release of substance P and acetylcholine during hypoxia.
Substance P and choline acetyltransferase have been localised in a small proportion of endothelial cells of rat coronary arteries using electron microscopic immunocytochemistry. During a hypoxic period of 1 min, coronary vasodilatation was produced in the Langendorff heart preparation and increased levels of substance P and acetylcholine were released into the perfusate. The possibility that these substances are released from endothelial cells during hypoxia and contribute to the hyperaemic response is discussed.
Milner
P
P
University College London, Department of Anatomy and Developmental Biology, United Kingdom.
Ralevic
V
V
Hopwood
A M
AM
Fehér
E
E
Lincoln
J
J
Kirkpatrick
K A
KA
Burnstock
G
G
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D002795:Choline O-Acetyltransferase / Q000032:analysis / Q000378:metabolism*; D003331:Coronary Vessels / Q000378:metabolism / Q000648:ultrastructure*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D008297:Male; D008854:Microscopy, Electron; D010100:Oxygen / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013373:Substance P / Q000032:analysis / Q000378:metabolism*
D013373:Substance P; D002795:Choline O-Acetyltransferase; D000109:Acetylcholine; D010100:Oxygen