An estradiol-dependent protein from chicken liver binds single-stranded DNA and RNA.
We have identified two estradiol-dependent single-stranded DNA binding proteins in the nucleus and cytoplasm of chicken hepatocytes that bind the sequence 5'TCACCTTCGCTATG3' in the first exon of the chicken vitellogenin gene. As judged by chromatography on heparin-Sepharose and by proteolytic clipping bandshift assay, the two proteins are different. Furthermore, they only bind to the oligonucleotide corresponding to the upper strand. Depurination and depyrimidination interference experiments with the cytoplasmic protein show that the bases CCTT-G are involved in the protein-DNA interaction. An RNA corresponding to the upper strand of the gene between nucleotide positions -73 and +53 competes for binding to the single-stranded DNA. UV cross-linking experiments performed with bromouridine-substituted single-stranded RNA reveal that an estradiol-dependent hepatocyte cytoplasmic protein with a Mr of 71,000 binds to the mRNA-like single-stranded RNA.
Feavers
I M
IM
Friedrich Miescher Institut, Basel, Switzerland.
McEwan
I J
IJ
Liang
H
H
Jost
J P
JP
D000818:Animals; D001667:Binding, Competitive; D002645:Chickens; D003593:Cytoplasm / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000302:isolation & purification / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D004958:Estradiol / Q000502:physiology*; D008099:Liver / Q000378:metabolism*; D008297:Male; D012313:RNA / Q000378:metabolism*; D014819:Vitellogenins / Q000235:genetics / Q000378:metabolism
D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D014819:Vitellogenins; D004958:Estradiol; D012313:RNA