Human nasal polyp epithelial basophil/mast cell and eosinophil colony-stimulating activity. The effect is T-cell-dependent.
We have previously reported highly potent basophil/mast cell (BMC) and basophil/eosinophil (Eo) colony-stimulating activities (CSA) in conditioned medium derived from cultured human nasal polyp epithelial scrapings (NP-CM). We now have examined the involvement of peripheral blood T-cells in the NP-CM stimulation of colony-forming units (cfu) from the blood of atopic and nonatopic subjects. Because the number of BMC- and Eo-cfu was significantly higher in cultures of peripheral blood from subjects with out-of-season ragweed allergic rhinitis than from control subjects (23.8 +/- 4.1 versus 9.0 +/- 2.4, p less than 0.01), we asked whether the observed colony stimulation could be a T-cell-dependent effect. Indeed, peripheral blood target cells consisting of a reconstituted mixture of T-cells and T-cell-depleted peripheral blood mononuclear cells (non-T-cells) yielded a significantly higher number of colonies in the presence of NP-CM than the non-T-cells alone. NP-CM did not stimulate colony formation by isolated T-cells. These observations point to interactions among nasal epithelial growth and differentiation factors, blood-borne progenitors and T-cells in the local accumulation of basophils, mast cells, and eosinophils in nasal polyps.
Ohnishi
M
M
Department of Medicine, McMaster University, Hamilton, Ontario, Canada.
Ruhno
J
J
Bienenstock
J
J
Milner
R
R
Dolovich
J
J
Denburg
J A
JA
D001491:Basophils / Q000473:pathology*; D002452:Cell Count; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay / Q000379:methods; D004804:Eosinophils / Q000473:pathology*; D004848:Epithelium / Q000473:pathology; D006801:Humans; D008407:Mast Cells / Q000473:pathology*; D009298:Nasal Polyps / Q000473:pathology*; D012221:Rhinitis, Allergic, Perennial / Q000473:pathology; D006255:Rhinitis, Allergic, Seasonal / Q000473:pathology; D013194:Staining and Labeling / Q000379:methods; D013234:Stem Cells / Q000473:pathology*; D013601:T-Lymphocytes / Q000473:pathology*