Sodium influxes in renal epithelial LLC-PK1/Cl4 cells monitored by 23Na NMR.
23Na NMR experiments with a suspension of the renal epithelial cell line, LLC-PK1/Cl4, in the presence of the shift reagent dysprosium polyphosphate showed that the intracellular sodium was only NMR visible for 64 +/- 4%. Intracellular sodium content was found to be 30.6 +/- 1.2 mM (25 degrees C). Examination of the sodium influx during recovery from intracellular acidification showed that sodium is transported not only by Na+/H+ exchange but also by sodium-D-glucose cotransport with a stoichiometry of 1:1.
Jans
A W
AW
Max-Planck-Institut fuer Systemphysiologie, Dortmund, Federal Republic of Germany.
Willem
R
R
Kellenbach
E R
ER
Kinne
R K
RK
D000136:Acid-Base Equilibrium / Q000187:drug effects; D000818:Animals; D002460:Cell Line; D004734:Energy Metabolism / Q000187:drug effects; D004848:Epithelium / Q000378:metabolism; D007424:Intracellular Fluid / Q000378:metabolism; D007473:Ion Channels / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D010042:Ouabain / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism*; D013552:Swine
D007473:Ion Channels; D010042:Ouabain; D012964:Sodium