Indoxyl-tetranitro blue tetrazolium method for detection of alkaline phosphatase in immunohistochemistry.
A sensitive method for detection of alkaline phosphatase in immunohistochemistry, using lymphoid cells, has been optimized. The conditions for staining are 0.23 mM 5-bromo-4-chloro-indoxyl phosphate, 0.55 mM tetranitro blue tetrazolium, 2.0 mM levamisole, 5.0 mM sodium azide, 10.0 mM magnesium chloride, and 0.15 mM 1-methoxyphenazine methosulfate dissolved in 100 mM Tris-HCl buffer, pH 9.5.
Brenan
M
M
Department of Immunology, John Curtin School of Medical Research, Australian National University, Canberra.
Bath
M L
ML
D000469:Alkaline Phosphatase / Q000032:analysis*; D000818:Animals; D007150:Immunohistochemistry; D007211:Indoles; D008221:Lymphoid Tissue / Q000201:enzymology; D009580:Nitroblue Tetrazolium; D012394:Rosaniline Dyes; D012756:Sheep; D013194:Staining and Labeling; D013778:Tetrazolium Salts
D007211:Indoles; D012394:Rosaniline Dyes; D013778:Tetrazolium Salts; C028130:tetranitrotetrazolium blue; D009580:Nitroblue Tetrazolium; C027071:new fuchsin; C035455:5-bromo-4-chloro-3-indoxyl phosphate; D000469:Alkaline Phosphatase