Dextran T-500 improves survival and spreading of chick embryo cells in serum-free medium.
The supplementation of serum-free Eagle's minimal essential medium (EMEM) with Dextran T-500 significantly improves attachment, spreading and survival of chick embryo cells in primary (myoblasts) and secondary (fibroblasts) cultures. These effects were observed in quiescent cultures incubated at 4 degrees C as well as at 37 degrees C. The results suggest that dextran can be used to simplify conditions for research on factors influencing cell proliferation and differentiation in serum-free media.
Pietrzkowski
Z
Z
Department of Cell Biology, Jan Zurzycki Institute of Molecular Biology, Jagellonian University, Kraków, Poland.
Reiss
K
K
Korohoda
W
W
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D002642:Chick Embryo / Q000166:cytology*; D003470:Culture Media; D003911:Dextrans / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology; D009132:Muscles / Q000166:cytology
D003470:Culture Media; D003911:Dextrans