Convergence of serotonin-, enkephalin- and substance P-like immunoreactive afferent fibers on single pudendal motoneurons in Onuf's nucleus of the cat: a light microscope study combining the triple immunocytochemical staining technique with the retrograde HRP-tracing method.
Convergence of serotonin (5-HT)-, enkephalin (ENK)-, and substance P (SP)-like immunoreactive (LI) afferent fibers on single pudendal motoneurons within Onuf's nucleus was demonstrated: pudendal motoneurons were retrogradely labeled with horseradish peroxidase applied to the pudendal nerve. Subsequently, 5-HT-LI fibers were stained by the immunoperoxidase method, and then ENK- and SP-LI fibers were stained by the double immunofluorescence method.
Tashiro
T
T
Department of Oral Anatomy (2nd Division), School of Dentistry, Hiroshima University, Japan.
Satoda
T
T
Matsushima
R
R
Mizuno
N
N
D001003:Anal Canal / Q000294:innervation*; D000818:Animals; D002415:Cats; D004745:Enkephalins / Q000378:metabolism*; D005260:Female; D006735:Horseradish Peroxidase; D007150:Immunohistochemistry; D008297:Male; D009046:Motor Neurons / Q000378:metabolism*; D009475:Neurons, Afferent / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism*; D013373:Substance P / Q000378:metabolism*; D014521:Urethra / Q000294:innervation*
D004745:Enkephalins; D012701:Serotonin; D013373:Substance P; D006735:Horseradish Peroxidase