Autoimmune sequence of streptococcal M protein shared with the intermediate filament protein, vimentin.
The crossreactivity of antibodies against a renal autoimmune epitope of Streptococcus pyogenes M protein with glomerular mesangial cells was investigated. The antibodies directed against the amino acid sequence Ile-Arg-Leu-Arg of the nephritogenic type 1 M protein reacted in a fibrillar pattern with mesangial cells cultured from isolated glomeruli. In Western blots of urea-extracted mesangial proteins, the antibodies reacted with a 56-kD protein. Monoclonal and polyclonal antibodies identified the 56-kD mesangial protein as vimentin. Two synthetic peptides of human vimentin containing the sequence Arg-Leu-Arg reacted with the autoimmune antibodies raised against a streptococcal M protein peptide. These results provide evidence that the intermediate filament protein vimentin shares autoimmune epitopes with streptococcal M protein.
Kraus
W
W
Veterans Administration Medical Center, Memphis, Tennessee.
Ohyama
K
K
Snyder
D S
DS
Beachey
E H
EH
D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial; D001324:Autoantigens / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000276:immunology*; D015153:Blotting, Western; D002352:Carrier Proteins; D002417:Cattle; D003429:Cross Reactions; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D007678:Kidney Glomerulus / Q000276:immunology; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000276:immunology; D051381:Rats; D014746:Vimentin / Q000276:immunology*
D000942:Antigens, Bacterial; D001324:Autoantigens; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D000939:Epitopes; D009842:Oligopeptides; D014746:Vimentin; C014689:streptococcal M protein