The enhanced association of keratin with hepatoma cell nuclei.
A monoclonal antibody to rat hepatoma keratin demonstrates a close association of intermediate filaments with the nucleus in hepatoma cells. Immunoblot analysis of nuclear fractions and immunofluorescence of nuclei both prepared by standard procedures, indicate that intermediate filament proteins are consistently present. Sodium citrate extraction of these preparations diminishes the amount of intermediate filament proteins but does not totally remove the antigenic moieties, suggesting a tight association of intermediate filaments with nuclei. The results from both immunoblot analysis and immunofluorescent localization demonstrate the increased amount of keratins associated with hepatoma cell nuclei.
Burkhardt
A L
AL
Department of Biochemistry, University of Vermont, College of Medicine, Burlington 05405.
Woodcock-Mitchell
J L
JL
Mitchell
J J
JJ
Chiu
J F
JF
D000818:Animals; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000032:analysis / Q000276:immunology; D002951:Citrates; D019343:Citric Acid; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D007382:Intermediate Filaments / Q000378:metabolism; D007633:Keratins / Q000378:metabolism*; D008099:Liver / Q000648:ultrastructure; D008114:Liver Neoplasms, Experimental / Q000648:ultrastructure*
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D002843:Chromatin; D002951:Citrates; D019343:Citric Acid; D007633:Keratins