Substance P induces granulocyte infiltration through degranulation of mast cells.
Substance P, a potent vasodilatory neuropeptide, is released from peripheral nerve endings of sensory neurons by various stimuli. Although in vitro incubation of rat and human mast cells with substance P causes their degranulation, it is not known whether inflammatory changes induced by substance P are mediated by degranulation of mast cells. We investigated this point by using genetically mast cell-deficient WBB6F1-W/Wv and WCB6F1-Sl/Sld mice. The s.c. injection of substance P induced degranulation of mast cells in the skin of WBB6F1-+/+ mice, and then a marked eosinophil infiltration around the degranulated mast cells. However, WBB6F1-W/Wv and WCB6F1-Sl/Sld mice showed little or no eosinophil infiltration in the skin after the injection of substance P. When the mast cell deficiency of WBB6F1-W/Wv mice was rescued either systemically by bone marrow transplantation or locally by injection of cultured mast cells, injection of substance P induced the infiltration of eosinophils, suggesting that substance P-induced eosinophil infiltration was mediated through degranulation of mast cells.
Matsuda
H
H
Department of Veterinary Surgery, College of Agriculture, University of Osaka Prefecture, Japan.
Kawakita
K
K
Kiso
Y
Y
Nakano
T
T
Kitamura
Y
Y
D000818:Animals; D002452:Cell Count; D002465:Cell Movement / Q000187:drug effects*; D004205:Cromolyn Sodium / Q000008:administration & dosage; D003594:Cytoplasmic Granules / Q000187:drug effects* / Q000276:immunology; D005260:Female; D006098:Granulocytes / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D007279:Injections, Subcutaneous; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D051379:Mice; D008817:Mice, Mutant Strains; D012867:Skin / Q000473:pathology; D013373:Substance P / Q000008:administration & dosage*; D003189:p-Methoxy-N-methylphenethylamine / Q000008:administration & dosage
D013373:Substance P; D003189:p-Methoxy-N-methylphenethylamine; D004205:Cromolyn Sodium