Genetic analysis of monoclonal antibody and HIV binding sites on the human lymphocyte antigen CD4.
Saturation mutagenesis and a complement fixation selection have yielded CD4 point mutants with impaired antibody and human immunodeficiency virus binding. The patterns of amino acid substitution, in conjunction with previous antibody cross-blocking data, affirm the similar tertiary structures of the CD4 amino-terminal domain and immunoglobulin variable regions. Single residue substitutions affecting virus binding and syncytium formation are observed over an eight residue segment located in a portion of the molecule homologous to the second hypervariable region of an antibody combining site.
Peterson
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Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
Seed
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D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001483:Base Sequence; D001666:Binding Sites, Antibody / Q000235:genetics; D001667:Binding, Competitive; D002460:Cell Line; D003168:Complement Fixation Tests; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006678:HIV / Q000235:genetics / Q000276:immunology*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D015728:Receptors, HIV; D011991:Receptors, Virus / Q000235:genetics* / Q000276:immunology*; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D000911:Antibodies, Monoclonal; D000939:Epitopes; D015728:Receptors, HIV; D011991:Receptors, Virus