[Reduction induced by substance P of the initial accumulation of myo(3H)inositol in acinar cells of the parotid gland in rats].
The stimulation of rat parotid acinar cells by substance P(SP) resulted in a significant reduction in the initial accumulation of cytosolic myo[3H]inositol and in the initial labelling of phosphoinositides. The SP-induced reduction was concentration-dependent, the EC50 of SP was 5.8 +/- 2.5 nM. Spantide, [D-Arg1, D-Trp7,9, Leu11]SP, a SP antagonist, used at a concentration of 10(-5) M, competitively shifted the dose-response curve of SP. The pharmacological analysis of the effects of several tachykinins and analogues, suggests the implication of NK1 receptors (specific receptor of SP).
Torrens
Y
Y
Collège de France, Chaire de Neuropharmacologie, I.N.S.E.R.M. n. U 114, Paris.
Dietl
M
M
Beaujouan
J C
JC
Glowinski
J
J
D000700:Analgesics / Q000494:pharmacology; D000818:Animals; D003600:Cytosol / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D007294:Inositol / Q000378:metabolism*; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism; D010306:Parotid Gland / Q000378:metabolism / Q000648:ultrastructure*; D051381:Rats; D011919:Rats, Inbred Strains; D013373:Substance P / Q000037:antagonists & inhibitors / Q000494:pharmacology*
D000700:Analgesics; D008563:Membrane Lipids; D013373:Substance P; D007294:Inositol
Réduction par la substance P de l'accumulation initiale du myo[3H]inositol dans les cellules acineuses de la glande parotide de rat.