RNA degrading activity is tightly associated with the multicatalytic proteinase, ingensin.
The multicatalytic proteinase, ingensin, was purified to homogeneity from chicken liver. rRNA-degrading activity was co-eluted with the purified multicatalytic proteinase from a TSK-3000SW column. This RNA-degrading activity was inactivated by heat treatment and the addition of a low concentration of SDS. Therefore, the RNA-degrading activity co-eluted with the multicatalytic proteinase was not due to contamination by low-molecular-mass RNases. These results strongly suggest that this RNA-degrading activity was tightly associated with the multicatalytic proteinase, ingensin.
Tsukahara
T
T
National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan.
Tanaka
K
K
Ogawa
T
T
Ishiura
S
S
Funabiki
R
R
Sugita
H
H
D000818:Animals; D002384:Catalysis; D002645:Chickens; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D006358:Hot Temperature; D008099:Liver / Q000201:enzymology*; D009097:Multienzyme Complexes / Q000302:isolation & purification / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex; D012313:RNA / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism
D009097:Multienzyme Complexes; D012333:RNA, Messenger; D012313:RNA; D012343:RNA, Transfer; D012260:Ribonucleases; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex