title: Isolation of a cDNA that hybrid selects antiproliferative mRNA from rat liver.
abstract: Studies of chromosome loss in inherited cancers, of fusions between proliferating and quiescent cells, and of microinjection of RNA from quiescent cells into proliferation competent cells have all provided evidence for antiproliferative genes in mammalian cells. In this report, we describe a partial cDNA clone isolated on the basis of its preferential hybridization to RNA from normal versus regenerating rat liver. The corresponding mRNA, enriched by hybrid selection, was microinjected into normal human diploid fibroblasts in cell culture, resulting in a 53% decrease in the fraction of nuclei incorporating tritiated thymidine. This mRNA is 2 kb in size and is expressed in eight tissues examined.
authors: S.R. Noble Foundation, Ardmore, OK 73402.
authors: J K
authors: JK
authors: McClung
authors: D B
authors: DB
authors: Danner
authors: D A
authors: DA
authors: Stewart
authors: J R
authors: JR
authors: Smith
authors: E L
authors: EL
authors: Schneider
authors: C K
authors: CK
authors: Lumpkin
authors: R T
authors: RT
authors: Dell'Orco
authors: M J
authors: MJ
authors: Nuell
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D004261:DNA Replication; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008115:Liver Regeneration; D008297:Male; D008845:Microinjections; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011061:Poly A / Q000302:isolation & purification; D012313:RNA / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D004247:DNA