Construction of a poliovirus type 1/type 2 antigenic hybrid by manipulation of neutralization antigenic site II.
There are three serotypes of poliovirus, poliovirus type 1 (PV-1), PV-2, and PV-3. These viruses each display four distinct neutralization antigenic sites, designated N-AgI, N-AgII, N-AgIIIA, and N-AgIIIB. It has been demonstrated previously that part of N-AgI can be replaced with heterogeneous amino acid sequences, resulting in hybrid viruses expressing heterogeneous antigenic determinants. To study whether hybrid viruses could be constructed by modifying another antigenic site, a part of N-AgII (amino acids 158 to 173 of VP2) of PV-1(Mahoney) was replaced with the equivalent sequence from PV-2(Lansing). The resulting hybrid was viable and expressed both PV-1 and PV-2 antigenic determinants. When inoculated into rabbits, the hybrid induced neutralizing antibodies against both PV-1 and PV-2, showing that amino acids 158 to 173 of VP2 are able to function as an antigenic site independent of the rest of N-AgII. Manipulation of N-AgII represents a useful alternative method for the production of hybrid polioviruses.
Murdin
A D
AD
Department of Microbiology, State University of New York, Stony Brook 11794-8621.
Wimmer
E
E
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D000939:Epitopes / Q000276:immunology; D005814:Genes, Viral; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D006824:Hybridization, Genetic; D007106:Immune Sera; D008969:Molecular Sequence Data; D009154:Mutation; D009500:Neutralization Tests; D010957:Plasmids; D017955:Poliovirus / Q000145:classification / Q000235:genetics / Q000276:immunology*; D011817:Rabbits; D012703:Serotyping; D014158:Transcription, Genetic; D014162:Transfection; D010948:Viral Plaque Assay; D015678:Viral Structural Proteins / Q000235:genetics
D000956:Antigens, Viral; D004279:DNA, Viral; D000939:Epitopes; D007106:Immune Sera; D015678:Viral Structural Proteins