A method for RNA isolation from marine macro-algae.
Sulfated, carboxylic polysaccharides and polyphenols found in marine macro-algae interfere with RNA isolation from these plants and inhibit RNA activities in vitro. Methods based on differential precipitation of RNA or carbohydrates in high salts were used to eliminate the acidic carbohydrates. To protect RNA from inactivation by oxidized polyphenols, strong reducing reagents were used to prevent polyphenol oxidation. RNA was successfully isolated from Macro-cystis pyrifera (brown alga), Porphyra schizophylla (red alga), and Enteromorpha intestinalis (green alga). mRNA isolated from the total RNA was shown to be translationally active.
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Department of Biological Sciences, University of California, Santa Barbara 93106.
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D002241:Carbohydrates / Q000302:isolation & purification; D000460:Chlorophyta / Q000032:analysis; D056890:Eukaryota / Q000032:analysis*; D008722:Methods; D000459:Phaeophyceae / Q000032:analysis; D014176:Protein Biosynthesis; D012313:RNA / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000235:genetics; D000461:Rhodophyta / Q000032:analysis
D002241:Carbohydrates; D012333:RNA, Messenger; D012313:RNA