Quantitative enzyme immunoassay for human granulocyte colony stimulating factor (G-CSF).
Human granulocyte colony stimulating factor (G-CSF) was detected at the pM level by a simple sequential sandwich enzyme immunoassay. The polyclonal antiserum obtained by immunizing rabbits with recombinant G-CSF did not recognize other colony stimulating factors. The EIA developed gave satisfactory reproducibility as judged by an intra-assay precision of 4.3-6.2% and an interassay precision of 6.2-9.0%. Results from this assay procedure correlated well with those of a bioassay. The method could be performed within 6 h.
Motojima
H
H
Applied Research Laboratories, Chugai Pharmaceutical Co., Ltd., Tokyo, Japan.
Kobayashi
T
T
Shimane
M
M
Kamachi
S
S
Fukushima
M
M
D000818:Animals; D000918:Antibody Specificity; D002460:Cell Line; D003115:Colony-Stimulating Factors / Q000097:blood* / Q000276:immunology / Q000592:standards; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor; D006801:Humans; D007106:Immune Sera / Q000032:analysis; D007124:Immunoenzyme Techniques / Q000592:standards; D010053:Ovary; D011817:Rabbits; D011994:Recombinant Proteins / Q000097:blood / Q000276:immunology / Q000592:standards; D012015:Reference Standards
D003115:Colony-Stimulating Factors; D007106:Immune Sera; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor