title: Modification of a trypsin-detergent method for DNA flow cytometry of human epidermis.
abstract: An existing technique (Vindelov et al. Cytometry 3:323, 1983) has been modified for DNA flow cytometry of human epidermis obtained from 2 to 3 mm punch biopsies. By varying the length of time of digestion of the epidermal disc by trypsin from 5 to 70 min a controlled release of keratinocytes occurred beginning with the stratum basale and proceeding toward, but not including, the superficial layer of the epidermis, the stratum corneum.
authors: Department of Dermatology, University of Arkansas for Medical Sciences, Little Rock 72205.
authors: D B
authors: DB
authors: Stough
authors: E R
authors: ER
authors: Burns
authors: S B
authors: SB
authors: Mallory
authors: J L
authors: JL
authors: Pipkin
authors: W G
authors: WG
authors: Hinson
mesh_terms: D002450:Cell Communication / Q000187:drug effects; D002467:Cell Nucleus / Q000032:analysis / Q000648:ultrastructure; D004247:DNA / Q000032:analysis*; D003902:Detergents; D000078404:Epidermal Cells; D004817:Epidermis / Q000032:analysis* / Q000648:ultrastructure; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007633:Keratins / Q000032:analysis; D008722:Methods; D013501:Surface-Active Agents; D014357:Trypsin / Q000494:pharmacology
chemical_list: D003902:Detergents; D013501:Surface-Active Agents; D007633:Keratins; D004247:DNA; D014357:Trypsin