Interleukin-6 is not involved in the interleukin-1-induced production of colony-stimulating factors by human bone marrow stromal cells and fibroblasts.
Interleukin-6 (IL-6) is a multifunctional cytokine that plays a role in regulation of hematopoiesis. Because IL-6 is coinduced with colony-stimulating factors (CSFs) by various cell types in response to stimulation with IL-1, we investigated whether IL-6 is involved in the IL-1-induced production of CSF by human bone marrow (BM) cells in long-term culture or human fibroblasts. We showed that IL-6 does not induce CSF production by these cells. Neither addition of exogenous IL-6 nor neutralization of endogenous production of IL-6 by an anti-IL-6 monoclonal antibody (MoAb) diminished the IL-1-induced colony-stimulating activity (CSA), indicating that IL-6 did not act synergistically with IL-1. Finally, IL-6 did not influence the kinetics of IL-1-induced CSA production by human fibroblasts. We conclude that IL-6, either alone or in combination with IL-1, does not induce CSF production by human BM stromal cells or fibroblasts.
Schaafsma
M R
MR
Department of Hematology, University Medical Center, Leiden, The Netherlands.
Fibbe
W E
WE
Van Damme
J
J
Duinkerken
N
N
Ralph
P
P
Kaushansky
K
K
Altrock
B W
BW
Willemze
R
R
Falkenburg
J H
JH
D001853:Bone Marrow / Q000502:physiology; D001854:Bone Marrow Cells; D003115:Colony-Stimulating Factors / Q000096:biosynthesis*; D005347:Fibroblasts; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000096:biosynthesis; D006801:Humans; D007158:Immunologic Techniques; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000502:physiology*; D016173:Macrophage Colony-Stimulating Factor
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D007375:Interleukin-1; D015850:Interleukin-6; D016179:Granulocyte Colony-Stimulating Factor; D016173:Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor