Characterization of in vivo salivary-derived enamel pellicle.
Salivary proteins and glycoproteins that participate in the formation of 2-h in vivo enamel pellicle were determined utilizing polyacrylamide gel electrophoresis [sodium dodecyl sulphate (SDS)-PAGE and anionic PAGE]/Western transfer analyses, and specific radiolabelling/SDS-PAGE fluorography. The sensitivity of these methods permitted the identification of individual members of different salivary protein families. The major components of this pellicle were salivary alpha-amylase, cysteine-containing phosphoprotein (CCP or cystatins), salivary mucin and sIgA. Glycosylated amylase was present in larger quantity than the non-glycosylated species. Only CCP1 (cystatin SA-I) of the cysteine-containing phosphoprotein family was identified. The higher molecular-weight salivary mucin (MG1), but not the lower molecular-weight species (MG2), was detected. These results extend earlier observations regarding the selective nature of salivary protein adsorption to enamel surface by demonstrating that only specific members of salivary protein families are involved in 2-h in vivo enamel pellicle formation. The findings also suggest that individual family members may have different functions in the mouth.
Al-Hashimi
I
I
Department of Oral Biology, School of Dental Medicine, State University of New York, Buffalo 14214.
Levine
M J
MJ
D000328:Adult; D000596:Amino Acids / Q000032:analysis; D000681:Amylases / Q000032:analysis; D015891:Cystatins / Q000032:analysis; D003741:Dental Deposits / Q000032:analysis*; D044622:Dental Pellicle; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000032:analysis; D008970:Molecular Weight; D009077:Mucins / Q000032:analysis; D012463:Saliva / Q000502:physiology; D055333:Salivary Cystatins; D012471:Salivary Proteins and Peptides / Q000032:analysis*
D000596:Amino Acids; C525172:CST2 protein, human; D015891:Cystatins; D006023:Glycoproteins; D007071:Immunoglobulin A, Secretory; D009077:Mucins; D055333:Salivary Cystatins; D012471:Salivary Proteins and Peptides; D000681:Amylases