[The effect of selected irritants on suspended keratinocytes using double vital staining].
Loss of vitality of keratinocytes after influence of strong allergens and irritants was examined. Cell viability was determined by trypan blue exclusion assay and eosintyrode solution. The results of the two methods harmonize essentially, but obviously, single noxa can lead to changes of the dye that exclude the use for an appropriate test. On principle, the method is suitable for testing the substances. The toxic final concentration should constantly be ascertained. Still, the results are recommended to be guaranteed through other methods.
Heise
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Heise
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Wohlrab
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D000818:Animals; D001548:Benzalkonium Compounds / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D002840:Chromates / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005557:Formaldehyde / Q000633:toxicity*; D006168:Guinea Pigs; D015603:Keratinocytes / Q000187:drug effects*; D011192:Potassium Dichromate / Q000633:toxicity*; D012967:Sodium Dodecyl Sulfate / Q000633:toxicity*; D013194:Staining and Labeling
D001548:Benzalkonium Compounds; D002840:Chromates; D005557:Formaldehyde; D012967:Sodium Dodecyl Sulfate; D011192:Potassium Dichromate
Untersuchungen zur Wirkung ausgewählter Noxen an suspendierten Keratinozyten mittels zweier Vitalfärbungen.