title: Structure and expression of a Manduca sexta larval cuticle gene homologous to Drosophila cuticle genes.
abstract: A genomic clone was isolated from the tobacco hornworm, Manduca sexta, by virtue of its similarity to a Drosophila larval cuticle gene. RNA analysis shows that this clone, B311, is expressed at times appropriate for a larval cuticle gene. Hybrid-selection experiments using B311 DNA show that it encodes a 14 x 10(3) Mr protein, LCP-14, which is precipitated by an antiserum to Manduca larval cuticle. We have sequenced both genomic and cDNA clones for the LCP-14 gene. A conceptual translation of the cDNA sequence shows that the LCP-14 protein is similar not only to another Manduca cuticle protein, but also to Drosophila, Sarcophaga and Hyalophora cecropia cuticle proteins. Since these proteins are found in flexible cuticle and have similar sequences, we conclude they are encoded by homologous genes.
authors: Department of Zoology, University of Washington, Seattle 98195.
authors: J E
authors: JE
authors: Rebers
authors: L M
authors: LM
authors: Riddiford
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004331:Drosophila melanogaster; D005796:Genes; D015151:Immunoblotting; D007814:Larva; D007915:Lepidoptera / Q000235:genetics*; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
chemical_list: D012313:RNA; D004247:DNA