Analysis of a transformed cell line using antisense c-fos RNA.
Simian sarcoma virus (SSV)-infected NIH-3T3 cells (SSV-NIH-3T3), express a homologue of platelet-derived growth factor, (PDGF) a powerful inducer of the c-fos gene. We have used these cells to test the hypothesis that autocrine stimulation by PDGF-like molecules leads to c-fos expression which is functional in the transformed phenotype. We have transfected SSV-NIH-3T3 cells with a c-fos antisense-RNA expression vector, pSVsof, or control plasmids. pSVsof-transfected cells exhibit markedly decreased c-fos mRNA and protein levels, restored density-dependent growth arrest and reduced (three of five clones) tumorigenicity compared to control lines. The results confirm that c-fos cooperates in the transformed phenotype of SSV-NIH-3T3 cells.
Mercola
D
D
La Jolla Cancer Research Foundation, CA 92037.
Westwick
J
J
Rundell
A Y
AY
Adamson
E D
ED
Edwards
S A
SA
D000818:Animals; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D005260:Female; D005822:Genetic Vectors; D051379:Mice; D008819:Mice, Nude; D010957:Plasmids; D011518:Proto-Oncogene Proteins / Q000032:analysis; D016760:Proto-Oncogene Proteins c-fos; D011519:Proto-Oncogenes; D012313:RNA / Q000235:genetics*; D016372:RNA, Antisense; D012333:RNA, Messenger / Q000037:antagonists & inhibitors; D012842:Sarcoma Virus, Woolly Monkey / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection
D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D016372:RNA, Antisense; D012333:RNA, Messenger; D012313:RNA