title: Molecular epidemiology and subgroup determination of bovine group A rotaviruses associated with diarrhea in dairy and beef calves.
abstract: The genome electropherotyping technique was used to examine group A rotaviral infections of diarrheic calves ranging from 1 to 85 days of age in 2 beef and 27 dairy herds. Coelectrophoresis studies demonstrated 38 distinct bovine group A rotavirus genome electropherotypes; all were long genome electropherotypes, and none had extra segments or unusual segment rearrangements. Genome electropherotypes in fecal specimens from diarrheic calves previously inoculated orally with a commercial, modified-live group A rotavirus vaccine differed from the vaccine genome electropherotype. Generally, when fecal specimens for genome electropherotyping were collected from two or more different calves within the same herd over a relatively short time, only one genome electropherotype was detected within a given herd. Different genome electropherotypes were detected in the same herd, however, when fecal specimens were obtained from different diarrheic calves over longer intervals (6 months or more). Twenty-three group A rotavirus strains with distinct genome electropherotypes, from diarrheic calves in 22 herds, were isolated and plaque purified in cell culture, and all were subgroup 1 group A rotaviruses. Non-group A rotavirus genome electropherotypes were not detected in 131 fecal specimens, negative for group A rotavirus, collected from diarrheic calves in 17 dairy herds.
authors: Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691.
authors: K W
authors: KW
authors: Theil
authors: C M
authors: CM
authors: McCloskey
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000382:microbiology*; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D005260:Female; D005455:Fluorescent Antibody Technique; D014644:Genetic Variation; D009820:Ohio; D012330:RNA, Double-Stranded / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis*; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000276:immunology; D012400:Rotavirus Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D012330:RNA, Double-Stranded; D012367:RNA, Viral