Active sensitization does not increase the responsivity of rat peritoneal mast cells to diverse chemical stimuli.
Peritoneal mast cells from specific pathogen-free rats that had been sensitized to the nematode Nippostrongylus brasiliensis released histamine in a dose-dependent fashion on challenge with antigen, anti-rat IgE, anti-rat IgG and concanavalin A. Cells from non-sensitized animals showed weak or insignificant responses to these agents. However, the two preparations did not obviously differ in their reactivity to a range of pharmacological agents including ATP, dextran, polyamines and calcium ionophores. Both populations were refractory to cholinergic stimulation. These results show that active sensitization does not per se lead to a global increase in mast cell reactivity or releasability.
Peachell
P T
PT
Department of Chemistry, University College London, UK.
Pearce
F L
FL
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D003911:Dextrans / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects; D007114:Immunization; D066298:In Vitro Techniques; D007476:Ionophores / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D009559:Nippostrongylus / Q000276:immunology; D011073:Polyamines / Q000494:pharmacology; D051381:Rats
D003911:Dextrans; D007476:Ionophores; D011073:Polyamines; D000255:Adenosine Triphosphate