Elevated level of p60c-src in virus-transformed murine megakaryocytic cell lines.
Megakaryocytic cell lines derived from mouse bone marrow cells transformed by the Myeloproliferative Leukemia Virus (MPLV) contain elevated levels of p60c-src. Northern blot analysis revealed the presence of a 4 kb normal sized c-src transcript only in MPLV-transformed megakaryocytic cell lines containing a high percentage of acetylcholinesterase positive cells (AChE+ greater than 10%), but not in MPLV-transformed erythroblastic or myeloblastic cell lines. The p60c-src protein was identified in lysates from in vivo labelled cells and in in vitro labelled membrane extracts by immunoblotting analysis and by immunoprecipitations with specific anti-src antibodies. In dimethylsulfoxide (DMSO) treated cells, the number of AChE+ cells increased together with p60c-src kinase activity indicating a possible correlation between p60c-src expression/activity and megakaryocytic differentiation.
Fischer
S
S
Unité 15 INSERM, CHU Cochin, Paris, France.
Wendling
F
F
Boulet
I
I
Cocault
L
L
Soula
M
M
Fagard
R
R
Tambourin
P
P
D000110:Acetylcholinesterase / Q000032:analysis; D000818:Animals; D001792:Blood Platelets / Q000032:analysis; D002460:Cell Line; D002472:Cell Transformation, Viral; D008533:Megakaryocytes / Q000032:analysis*; D051379:Mice; D008810:Mice, Inbred C57BL; D011505:Protein-Tyrosine Kinases / Q000032:analysis; D011518:Proto-Oncogene Proteins / Q000032:analysis*; D016392:Proto-Oncogene Proteins pp60(c-src); D014158:Transcription, Genetic
D011518:Proto-Oncogene Proteins; D011505:Protein-Tyrosine Kinases; D016392:Proto-Oncogene Proteins pp60(c-src); D000110:Acetylcholinesterase