Molecular cloning of a gene encoding a Chlamydia psittaci 57-kDa protein that shares antigenic determinants with ca. 60-kDa proteins present in many gram-negative bacteria.
In order to develop reagents to study the immune response of guinea pigs to infection by Chlamydia psittaci guinea pig inclusion conjunctivitis strain (GPIC), we constructed a plasmid clone bank with C. psittaci DNA. One of the recombinant clones isolated produced large amounts of a 57-kilodalton (kDa) protein that was immunoreactive with sera from GPIC infected guinea pigs. While investigating this recombinant protein, we discovered that all the Gram-negative bacteria analyzed so far have immunoreactive proteins of similar size. This protein seems to be a 'common antigen' already described in various Gram-negative bacteria.
Menozzi
F D
FD
Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, MT 59840.
Menozzi-Dejaiffe
C
C
Nano
F E
FE
D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology; D002691:Chlamydophila psittaci / Q000235:genetics*; D003001:Cloning, Molecular; D000939:Epitopes / Q000032:analysis*; D006090:Gram-Negative Bacteria / Q000276:immunology*; D013210:Staphylococcus / Q000276:immunology; D015003:Yeasts / Q000276:immunology
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D000939:Epitopes