Expression of an enzymatically active murine retroviral reverse transcriptase in human cells.
The region of the pol gene of the Moloney murine leukemia virus (M-MuLV) encoding the reverse transcriptase and RNase H activities was inserted in an eukaryotic expression vector and transiently expressed in human cultured cells. This results in the expression of high levels of reverse transcriptase activity. This enzyme, partially purified, also carries a RNase H activity, has the biochemical requirements of the viral enzyme and is recognized and inhibited by antibodies directed against a M-MuLV reverse transcriptase expressed in Escherichia coli.
Jean-Jean
O
O
Laboratoire de Biologie Moléculaire de la Réplication, ER 272-CNRS, IRSC, Villejuif, France.
Moyret
C
C
Bernard
D
D
de Recondo
A M
AM
Rossignol
J M
JM
D000818:Animals; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004722:Endoribonucleases / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D005818:Genetic Engineering; D006801:Humans; D007158:Immunologic Techniques; D051379:Mice; D010957:Plasmids; D012194:RNA-Directed DNA Polymerase / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid; D016914:Ribonuclease H; D013997:Time Factors
D012194:RNA-Directed DNA Polymerase; D004722:Endoribonucleases; D016914:Ribonuclease H