Effect of age and of hypertrophy on cardiac Ca2+ antagonist binding sites.
We explored the effect of age and of hypertrophy on Ca2+ antagonist binding site density (Bmax), affinity (Kd), and selectivity in cardiac membranes harvested from the hearts of young adult (9-week-old) and older (25-week-old) Sprague Dawley (SD) rats, Wistar Kyoto rats (WKY), and spontaneously hypertensive rats (SHR). Radioligand binding studies with (+)[3H]PN200-110 failed to show a significant difference between the Bmax obtained for the cardiac membranes of 9-week-old SD, WKY, or SHR. Similarly, at 25 weeks, the Bmax values were the same for each group, but in each group the Bmax tended to increase with age. The Kd and selectivity were unchanged. For (-)[3H]D888 binding, the Kd values changed with age, but there was no hypertension or hypertrophy-linked increase in Bmax. In the SD and SHR series, but not in the WKY, there was a tendency for the Bmax to increase with age. We interpreted these results to mean that age may contribute to the different Kd and Bmax values described for cardiac membranes from 25-week-old WKY and SHR.
Dillon
J S
JS
Department of Medicine, University of Melbourne, Victoria, Australia.
Gu
X H
XH
Nayler
W G
WG
D000375:Aging / Q000378:metabolism*; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D015220:Calcium Channels; D006332:Cardiomegaly / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004095:Dihydropyridines / Q000378:metabolism; D007202:Indicators and Reagents; D017275:Isradipine; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D010069:Oxadiazoles / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D011919:Rats, Inbred Strains; D011921:Rats, Inbred WKY; D011978:Receptors, Nicotinic / Q000378:metabolism*; D013997:Time Factors; D014700:Verapamil / Q000031:analogs & derivatives / Q000378:metabolism
D015220:Calcium Channels; D004095:Dihydropyridines; D007202:Indicators and Reagents; D010069:Oxadiazoles; D011978:Receptors, Nicotinic; C043184:4-desmethoxyverapamil; D014700:Verapamil; D017275:Isradipine