Detection of turnip crinkle virus on agarose gel electropherograms at the nanogram load level.
The previous conditions for the physical characterization of turnip crinkle virus (TCV) by quantitative agarose gel electrophoresis [1, 2] were limiting the method to the microgram load level and were therefore insufficiently sensitive to satisfy the need in many areas of virology for detection of viruses containing single-stranded RNA at the nanogram level. The present report remedies that defect by presenting a technique compatible with the nanogram load level of such viruses. The technique is based on a reduction of gel thickness and on the use of silver staining.
Orbán
L
L
Section on Macromolecular Analysis, National Institute of Child Health and Human Development, Bethesda, MD 20892.
Chrambach
A
A
D004587:Electrophoresis, Agar Gel / Q000295:instrumentation; D004996:Ethidium; D005404:Fixatives; D010942:Plant Viruses / Q000032:analysis*; D012834:Silver; D013194:Staining and Labeling
D005404:Fixatives; D012834:Silver; D004996:Ethidium