Effects of recombinant G-CSF and GM-CSF on in vitro differentiation of the blast cells of RAEB and RAEB-T.
To evaluate the effects of recombinant G-CSF and GM-CSF on RAEB and RAEB-T cells, blast cells from 6 patients were incubated in liquid culture systems with these CSFs for 7 days, and their numerical, morphological and functional changes were assessed. Both CSFs stimulated cell growth, but decreased the proportion of blast cells in 5 of the 6 cases. Karyotypic abnormalities persisted during cultivation in some cases. The CSFs also stimulated the expression of part of the esterase activities, and a positive interaction of both CSFs was seen in part. Although CSFs had no significant effects on the ability of cells to reduce NBT or to phagocytize latex particles, the results indicated that they induce partial differentiation of blast cells. It appears that such pathological cells still retain the capacity to respond to growth factors.
Tohyama
K
K
First Division of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.
Ohmori
S
S
Michishita
M
M
Ueda
T
T
Ueda
Y
Y
Sakoda
H
H
Yoshida
Y
Y
Uchino
H
H
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000754:Anemia, Refractory, with Excess of Blasts / Q000473:pathology*; D001773:Blood Cells / Q000473:pathology; D001853:Bone Marrow / Q000473:pathology; D002452:Cell Count; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002648:Child; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D004950:Esterases / Q000032:analysis; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006098:Granulocytes; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D007621:Karyotyping; D008297:Male; D008863:Microspheres; D008875:Middle Aged; D009580:Nitroblue Tetrazolium; D010587:Phagocytosis; D011994:Recombinant Proteins / Q000494:pharmacology; D013194:Staining and Labeling
D003115:Colony-Stimulating Factors; D006133:Growth Substances; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D009580:Nitroblue Tetrazolium; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D004950:Esterases