Purified hepatocyte nuclear factor 1 interacts with a family of hepatocyte-specific promoters.
During development cell types arise through the activation or repression of classes of specific genes. One hypothesis is that this phenomenon is realized by tissue-specific factors playing a role at the transcription level. Recently we have described a liver-specific nuclear protein, hepatocyte nuclear factor 1, that appears to be involved in the transcription of the fibrinogen and alpha 1-antitrypsin genes. In this report we describe the purification of hepatocyte nuclear factor 1 and demonstrate that it interacts with essential promoter regions of many liver-specific genes, including albumin, alpha-fetoprotein, and transthyretin. This finding suggests that hepatocyte nuclear factor 1 could be one factor necessary for establishing the liver phenotype. We also show that this protein binds to the promoter of the surface-antigen gene of the hepatitis B virus, a virus characterized by a high degree of hepatotropism.
Courtois
G
G
Department of Pathology, Stanford University School of Medicine, CA 94305.
Baumhueter
S
S
Crabtree
G R
GR
D000418:Albumins / Q000235:genetics; D000818:Animals; D000954:Antigens, Surface / Q000235:genetics; D001665:Binding Sites; D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005340:Fibrinogen / Q000235:genetics; D006801:Humans; D008099:Liver / Q000032:analysis*; D051379:Mice; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D011228:Prealbumin / Q000235:genetics; D011401:Promoter Regions, Genetic; D051381:Rats; D014157:Transcription Factors; D014158:Transcription, Genetic; D051840:Y-Box-Binding Protein 1; D000515:alpha 1-Antitrypsin / Q000235:genetics; D000509:alpha-Fetoproteins / Q000235:genetics
D000418:Albumins; D000954:Antigens, Surface; D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D051839:NFI Transcription Factors; D009687:Nuclear Proteins; D011228:Prealbumin; D014157:Transcription Factors; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human; D000515:alpha 1-Antitrypsin; D000509:alpha-Fetoproteins; D005340:Fibrinogen