title: Regulated polyadenylation controls mRNA translation during meiotic maturation of mouse oocytes.
abstract: The translational activation of dormant tissue-type plasminogen activator mRNA during meiotic maturation of mouse oocytes is accompanied by elongation of its 3'-poly(A) tract. Injected RNA fragments that correspond to part of the 3'-untranslated region (3'UTR) of this mRNA are also subject to regulated polyadenylation. Chimeric mRNAs containing part of this 3'UTR are polyadenylated and translated following resumption of meiosis. Polyadenylation and translation of chimeric mRNAs require both specific sequences in the 3'UTR and the canonical 3'-processing signal AAUAAA. Injection of 3'-blocked mRNAs and in vitro polyadenylated mRNAs shows that the presence of a long poly(A) tract is necessary and sufficient for translation. These results establish a role for regulated polyadenylation in the post-transcriptional control of gene expression.
authors: Institute of Histology and Embryology, University of Geneva Medical School, Switzerland.
authors: J D
authors: JD
authors: Vassalli
authors: J
authors: J
authors: Huarte
authors: D
authors: D
authors: Belin
authors: P
authors: P
authors: Gubler
authors: A
authors: A
authors: Vassalli
authors: M L
authors: ML
authors: O'Connell
authors: L A
authors: LA
authors: Parton
authors: R J
authors: RJ
authors: Rickles
authors: S
authors: S
authors: Strickland
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002678:Chimera / Q000235:genetics; D008540:Meiosis; D051379:Mice; D008969:Molecular Sequence Data; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D011061:Poly A / Q000378:metabolism*; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D010959:Tissue Plasminogen Activator / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D010959:Tissue Plasminogen Activator