Immunohistochemical study of neuronal markers in human gingiva with phenytoin-induced overgrowth.
The immunohistochemical occurrence of several different neuronal markers has been investigated in human gingiva with phenytoin-induced overgrowth. The material was compared to gingival material taken following surgical orthodontic treatment. Gingiva obtained from the phenytoin-treated groups seemed to have a reduced number of neurofilament (NF) immunoreactive nerve fibers in the propria compared to control material. In both phenytoin as well as control gingiva sparsely distributed, thin, calcitonin gene-related peptide (CGRP) and substance P (SP) immunoreactive fibers were found in the propria. No obvious differences between the two groups could be observed for CGRP and SP. Immunoreactive cells for somatostatin (SOM) with a dendritic cell shape were found in the propria in both groups, sometimes in densely packed clusters. A tendency for increase of SOM-immunoreactive cells in the phenytoin-treated gingiva was observed. A few gamma-melanocyte stimulating hormone (gamma-MSH)-immunoreactive cells with a round appearance were found in control as well as phenytoin-affected gingiva. In one instance, however, a heavy gamma-MSH-immunoreactive cell infiltration was seen in the phenytoin sample. No immunoreactivity in either the phenytoin-treated group or in the control group was seen for proctolin or galanin. The results indicated that gingiva with phenytoin-induced overgrowth has a reduced innervation density revealed with NF immunohistochemistry.
Luthman
J
J
Department of Histology and Neurobiology, Karolinska Institutet, Stockholm, Sweden.
Dahllöf
G
G
Modèer
T
T
Johansson
O
O
D000293:Adolescent; D002116:Calcitonin / Q000032:analysis; D015740:Calcitonin Gene-Related Peptide; D002648:Child; D005881:Gingiva / Q000294:innervation*; D005885:Gingival Hyperplasia / Q000139:chemically induced / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D009074:Melanocyte-Stimulating Hormones / Q000032:analysis; D009412:Nerve Fibers / Q000648:ultrastructure*; D009454:Neurofibrils / Q000648:ultrastructure; D009479:Neuropeptides / Q000032:analysis; D010672:Phenytoin / Q000009:adverse effects*; D013004:Somatostatin / Q000032:analysis; D013373:Substance P / Q000032:analysis
D009479:Neuropeptides; D013373:Substance P; D013004:Somatostatin; D010672:Phenytoin; D009074:Melanocyte-Stimulating Hormones; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide