Synergistic effect of dolichyl phosphate and human recombinant granulocyte colony-stimulating factor on recovery from neutropenia in mice treated with anti-cancer drugs.
Severe hematopoietic injury in mice was induced by using either 5-fluorouracil, adriamycin, mitomycin C, or vinblastine. Daily subcutaneous administration of purified human recombinant granulocyte colony-stimulating factor (rG-CSF; 0.3-10.0 micrograms/day) markedly accelerated recovery from the drug-induced granulocytopenia in a dose-dependent manner, as reported previously. On the other hand, daily intraperitoneal administration of dolichyl phosphate (Dol-P) also enhanced granulopoiesis to accelerate recovery from granulocytopenia, although the effect of Dol-P was relatively moderate as compared with that of rG-CSF. A synergistic recovery of granulopoiesis was observed when Dol-P was administered together with rG-CSF to the mice treated with anti-cancer drugs. Joint use of Dol-P (1 mg/day) and rG-CSF (0.3 micrograms/day) was as effective as a higher dose of rG-CSF (3 micrograms/day). Joint use of Dol-P (1 mg/day) and rG-CSF (3 micrograms/day) was sometimes more effective.
Shimamura
M
M
Central Research Laboratories, Kuraray Company, Okayama, Japan.
Takigawa
T
T
Urabe
A
A
Okabe
T
T
Souza
L M
LM
Takaku
F
F
D000380:Agranulocytosis / Q000628:therapy*; D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D003115:Colony-Stimulating Factors / Q000008:administration & dosage*; D004288:Dolichol Phosphates / Q000008:administration & dosage*; D004317:Doxorubicin / Q000633:toxicity; D004334:Drug Administration Schedule; D004357:Drug Synergism; D005260:Female; D005472:Fluorouracil / Q000633:toxicity; D016179:Granulocyte Colony-Stimulating Factor; D006801:Humans; D051379:Mice; D009503:Neutropenia / Q000139:chemically induced / Q000188:drug therapy / Q000628:therapy*; D011106:Polyisoprenyl Phosphates / Q000008:administration & dosage*; D011994:Recombinant Proteins / Q000008:administration & dosage*; D014747:Vinblastine / Q000633:toxicity
D000970:Antineoplastic Agents; D003115:Colony-Stimulating Factors; D004288:Dolichol Phosphates; D011106:Polyisoprenyl Phosphates; D011994:Recombinant Proteins; C028306:dolichol monophosphate; D016179:Granulocyte Colony-Stimulating Factor; D014747:Vinblastine; D004317:Doxorubicin; D005472:Fluorouracil