Simultaneous isolation of high molecular weight RNA and DNA from limited amounts of tissues and cells.
A simple method is described for the simultaneous isolation of both DNA and RNA from tissues and cultured cells obtainable in limited quantities only. The method is based on a suitable combination of steps designed for preparations of high molecular weight nucleic acids in cases when restricted amounts of tissues like small-sized and unique biopsies of tumors are available for studies of gene organization and expression. Using this protocol, undegraded total RNA suitable for Northern blot analysis and high molecular weight DNA for Southern blots was obtained from various sources (mammary and colon carcinomas, meningiomas, colonic and placental tissue, and several cell cultures).
Meese
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E
Institute of Human Genetics, University of the Saar, Homburg, FRG.
Blin
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N
D015139:Blotting, Southern; D001943:Breast Neoplasms / Q000032:analysis; D002499:Centrifugation, Density Gradient / Q000379:methods; D004247:DNA / Q000302:isolation & purification*; D004273:DNA, Neoplasm / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D008577:Meningeal Neoplasms / Q000032:analysis; D008579:Meningioma / Q000032:analysis; D008970:Molecular Weight; D010920:Placenta / Q000032:analysis*; D011247:Pregnancy; D012313:RNA / Q000302:isolation & purification*; D012334:RNA, Neoplasm / Q000302:isolation & purification*
D004273:DNA, Neoplasm; D007202:Indicators and Reagents; D012334:RNA, Neoplasm; D012313:RNA; D004247:DNA