IL-1 production by T6 (CD1a) positive cord blood mononuclear cells (Langerhan's cell precursors?).
Human cord blood (CB) mononuclear cells were fractionated into peanut agglutinin positive (PNA+) and PNA negative (PNA-) subsets. The PNA+ subset was enriched for T6+(CD1a)Ia+ cells, which we have previously shown to resemble the Langerhans cells (LCs) of the skin, and therefore described as circulating LCs precursors. Supernatants of PNA+ and PNA- cells, and of FACS purified populations of T6+ CB cells, cultured with and without LPS, were tested for IL-1 activity. It was found that cord blood PNA+ mononuclear cells as well as purified populations of T6+ CB cells produce significant amounts of, both extracellular and cell associated, IL-1 as compared to PNA- and T6- cells, and comparable to those produced by macrophages. LPS stimulation mainly affected T6+ cells. It can be concluded that cord blood T6+ cells, presumably LCs precursors, are capable of IL-1 production.
Gothelf
Y
Y
Tissue Typing Laboratory, Chaim Sheba Medical Center, Tel Hashomer, Israel.
Dinarello
C A
CA
Yamin
M
M
Sharon
N
N
Milner
Y
Y
Gazit
E
E
D018949:Antigens, CD1; D000943:Antigens, Differentiation; D002469:Cell Separation; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D007700:Kinetics; D007801:Langerhans Cells / Q000378:metabolism*; D037102:Lectins; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D019887:Peanut Agglutinin
D018949:Antigens, CD1; D000943:Antigens, Differentiation; D007375:Interleukin-1; D037102:Lectins; D019887:Peanut Agglutinin