Azelaic acid: its uptake and mode of action in Staphylococcus epidermidis NCTC 11047.
In vitro tests using Staphylococcus epidermidis as a model have shown that at pH 5.6 the micro-organisms are sensitive to azelaic acid, whilst at pH 6.0 and 7.0 the cells become progressively resistant, especially with nutrients present. In a simple defined medium the growth rate was reduced at 1 mmol/l and growth inhibited at 25 mmol/l. The uptake of azelaic acid was pH dependent, higher transport at lower pH values, and required viable cells. Azelaic acid, 457 mumol/l gave 50% inhibition of protein synthesis and this mechanism could account for the bactericidal and bacteristatic effects. DNA and RNA were affected slightly by 100 mmol/l azelaic acid, and respiration by 500 mmol/l.
Bojar
R A
RA
Department of Microbiology, Leeds University, UK.
Holland
K T
KT
Leeming
J P
JP
Cunliffe
W J
WJ
D002250:Carbon Radioisotopes; D003470:Culture Media / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D003998:Dicarboxylic Acids / Q000493:pharmacokinetics* / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D010101:Oxygen Consumption / Q000187:drug effects; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*
D002250:Carbon Radioisotopes; D003470:Culture Media; D003998:Dicarboxylic Acids; D012313:RNA; D004247:DNA; C010038:azelaic acid