The autoradiographic localization of calcitonin gene-related peptide and substance P receptors in human fallopian tube.
The binding of [125I] human alpha calcitonin gene-related peptide (hCGRP) and [125I]Bolton-Hunter-labelled substance P (BH-SP) to human fallopian tube tissue sections was characterized and the respective binding sites were localized by light-microscopic autoradiography. The hCGRP binding sites were associated with the muscularis, lamina propria and vascular smooth muscle, the latter being the most intensely labelled. BH-SP binding sites were associated only with blood vessels. This labelling was intense, and appeared to be restricted to the vascular endothelium. These results suggest that calcitonin gene-related peptide and substance P may act together to regulate local blood flow and plasma extravasation.
Nimmo
A J
AJ
Department of Physiology, University of Leeds.
Whitaker
E M
EM
Carstairs
J R
JR
Morrison
J F
JF
D001345:Autoradiography; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D004730:Endothelium, Vascular / Q000032:analysis; D005187:Fallopian Tubes / Q000032:analysis* / Q000098:blood supply; D005260:Female; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000032:analysis; D018003:Receptors, Calcitonin; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000378:metabolism; D018040:Receptors, Neurokinin-1; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D013373:Substance P / Q000378:metabolism; D014018:Tissue Distribution
D018003:Receptors, Calcitonin; D011956:Receptors, Cell Surface; D018040:Receptors, Neurokinin-1; D017981:Receptors, Neurotransmitter; D013373:Substance P; D015740:Calcitonin Gene-Related Peptide