title: Peroxisomes in regenerating human skeletal myofibers.
abstract: Peroxisomes of the human regenerating skeletal myofibers were studied qualitatively and quantitatively by electron cytochemistry and were compared with those of the mature normal human skeletal muscle fibers. Peroxisomes visualized by electron cytochemistry with 3,3'-diaminobenzidine (DAB) were small round or oval bodies delimited by a single membrane and contained the electron-opaque, coarsely granular matrix. Muscle grafts of the regenerating normal human quadriceps obtained from 4 orthopedic patients were analyzed 2 weeks after transplantation into nude mice; they contained peroxisomes with a mean diameter of 0.25 microns, ranging from 0.12 to 0.67 microns. The group mean density of peroxisomes per 100 microns2 was 2.0 +/- 0.4 (SE), while that of histochemically normal mature human quadriceps femoris myofibers was 0. The cytochemical controls without DAB or with the presence of 3-amino 1,2,4-triazole in the solution containing DAB lacked the electron-opaque reaction, indicating that these reactions were on an enzymatic basis. The results of this study showed clearly that the regenerating normal human skeletal myofibers contained numerous peroxisomes differing from the mature normal human muscle fibers in which the peroxisomes were not observed.
authors: Department of Medicine, Showa University Fujigaoka Hospital, Yokohama, Japan.
authors: Y
authors: Y
authors: Wakayama
mesh_terms: D015100:3,3'-Diaminobenzidine; D000818:Animals; D002374:Catalase / Q000032:analysis; D005227:Fatty Acids / Q000378:metabolism; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008830:Microbodies / Q000201:enzymology / Q000648:ultrastructure*; D009132:Muscles / Q000502:physiology* / Q000637:transplantation / Q000648:ultrastructure; D012038:Regeneration / Q000502:physiology*; D013194:Staining and Labeling; D014183:Transplantation, Heterologous
chemical_list: D005227:Fatty Acids; D015100:3,3'-Diaminobenzidine; D002374:Catalase