Cloning by functional expression of a member of the glutamate receptor family.
We have isolated a complementary DNA clone by screening a rat brain cDNA library for expression of kainate-gated ion channels in Xenopus oocytes. The cDNA encodes a single protein of relative molecular mass (Mr) 99,800 which on expression in oocytes forms a functional ion channel possessing the electrophysiological and pharmacological properties of the kainate subtype of the glutamate receptor family in the mammalian central nervous system.
Hollmann
M
M
Molecular Neurobiology Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037.
O'Shea-Greenfield
A
A
Rogers
S W
SW
Heinemann
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D001483:Base Sequence; D001923:Brain Chemistry; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004594:Electrophysiology; D015870:Gene Expression; D005971:Glutamates / Q000494:pharmacology; D018698:Glutamic Acid; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology; D007608:Kainic Acid / Q000378:metabolism / Q000494:pharmacology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016202:N-Methylaspartate; D009865:Oocytes / Q000378:metabolism; D010957:Plasmids; D012313:RNA / Q000032:analysis; D051381:Rats; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter / Q000235:genetics*; D014018:Tissue Distribution; D014158:Transcription, Genetic; D014162:Transfection; D014981:Xenopus
D005971:Glutamates; D007473:Ion Channels; D017470:Receptors, Glutamate; D017981:Receptors, Neurotransmitter; D001224:Aspartic Acid; D018698:Glutamic Acid; D012313:RNA; D016202:N-Methylaspartate; D004247:DNA; D007608:Kainic Acid