Interleukin-1 alpha mRNA induced by cycloheximide PMA, and retinoic acid is reduced by dexamethasone in PAM-212 keratinocytes.
Keratinocytes in culture produce detectable amounts of IL-1 alpha mRNA constitutively and can be stimulated to express increased amounts of IL-1 alpha mRNA by cycloheximide, PMA, and retinoic acid. Dexamethasone decreases the amount of IL-1 mRNA induced by these agents, as well as constitutive IL-1 alpha mRNA. RU 486, which interferes with glucocorticosteroid-receptor binding, decreases inhibition of TPA stimulation of IL-1 alpha mRNA by dexamethasone, which suggests that the inhibition by dexamethasone is through a conventional ligand-receptor mechanism.
McGuire
J
J
Department of Dermatology, Yale University School of Medicine, New Haven, Connecticut 06510.
Langdon
R
R
Birchall
N
N
Kupper
T
T
D000818:Animals; D002461:Cell Line, Transformed; D003513:Cycloheximide / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology*; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism*; D007375:Interleukin-1 / Q000378:metabolism*; D007633:Keratins; D012333:RNA, Messenger / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D014212:Tretinoin / Q000037:antagonists & inhibitors / Q000494:pharmacology*
D007375:Interleukin-1; D012333:RNA, Messenger; D014212:Tretinoin; D007633:Keratins; D003907:Dexamethasone; D003513:Cycloheximide; D013755:Tetradecanoylphorbol Acetate