title: Cytotoxic T lymphocyte recognition of secreted HLA class I molecules.
abstract: The cytolytic responses of DBA/2 mice against syngeneic transfected P815 mastocytoma cells expressing either membrane-associated (HLA-Cw3) or -secreted hybrid (HLA-Cw3 x H-2 Q10b) molecules were compared. In spite of the absence of serologically detectable hybrid molecules on their plasma membrane, cells secreting these molecules elicited a CTL response similar to that of cells expressing the membrane associated HLA-Cw3 molecules, in terms of both MHC-restriction and peptide specificity. Together with the observation that syngeneic mice were capable of rejecting the injected secreting cells, these results imply that secreted HLA class I molecules can function as minor histocompatibility Ag and suggest that processing of both the membrane-bound and the -secreted forms of a protein may follow common or overlapping pathways.
authors: Centre d'Immunologie de Marseille-Luminy, France.
authors: B
authors: B
authors: Kahn-Perles
authors: C
authors: C
authors: Barra
authors: J
authors: J
authors: Jehan
authors: D
authors: D
authors: Fourel
authors: M
authors: M
authors: Barad
authors: J L
authors: JL
authors: Maryanski
authors: F A
authors: FA
authors: Lemonnier
mesh_terms: D000818:Animals; D002460:Cell Line; D002999:Clone Cells / Q000276:immunology / Q000637:transplantation; D000939:Epitopes / Q000235:genetics; D005813:Genes, Synthetic; D006680:HLA Antigens / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D015236:HLA-C Antigens / Q000235:genetics; D006801:Humans; D008297:Male; D012515:Mast-Cell Sarcoma / Q000378:metabolism; D051379:Mice; D008811:Mice, Inbred DBA; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000637:transplantation; D014162:Transfection
chemical_list: D000939:Epitopes; D006680:HLA Antigens; D015236:HLA-C Antigens; C038653:HLA-C*03 antigen