[Effect on erythropoietin production after inhibition of RNA synthesis by D-actinomycin and hyperglycemia].
Short-lasting hypobaric hypoxia was used in male white rats as a stimulus of erythropoietin synthesis (0.42 atm.). Plasma erythropoietin activity was determined by a biologic method on highly sensitive to exogenous erythropoietin recipients-posthypoxic polycytemic mice. Erythropoietin activity was estimated by measuring the percentage of incorporated 59Fe in newly formed erythrocytes of mice under the influence of the examined plasma, using automatic scintillation gamma-counter. Hypoxia induced an increase of plasma erythropoietin activity with 54% in comparison with control animals. Administration of actinomycin D in a dose of 0.4 microgram/g of body mass before exposure of the animals to hypoxia diminished production of erythropoietin with 58% in comparison with that of rats, exposed to pure hypoxia. A suggestion could be made that the formation of erythropoietin requires DNA dependent RNA-synthesis.
Kalaĭdzhieva
V
V
Nikolov
V
V
Logofetov
A
A
D000818:Animals; D003609:Dactinomycin / Q000494:pharmacology*; D004921:Erythropoietin / Q000096:biosynthesis* / Q000097:blood; D006943:Hyperglycemia / Q000097:blood*; D000860:Hypoxia / Q000097:blood; D008297:Male; D012313:RNA / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains
D004921:Erythropoietin; D003609:Dactinomycin; D012313:RNA
Povliiavane na eritropoetinovata produktsiia sled inkhibirane na RNK sinteza chrez D-aktinomitsin i pri khiperglikemiia.