Triiodothyronine nuclear binding capacity in rat tissues correlates with a 6.0 kilobase (kb) and not a 2.6 kb messenger ribonucleic acid hybridization signal generated by a human c-erbA probe.
Recent studies have raised the possibility of multiple structurally distinctive tissue-specific nuclear T3 receptors, all exhibiting homology with the v-erbA oncogene and represented by mRNAs of various sizes. We have assayed the level of mRNAs recognized by a 32P-labeled cRNA derived from human plancetal c-erbA-A beta cDNA by solution hybridization and by Northern transfer in different rat tissues, as well as human liver and placenta. Two related mRNAs were demonstrated in the rat tissues analyzed, one measuring 6.0 and the other 2.6 kilobases (kb). In human liver and placenta a 6.0 kb mRNA was seen, but not a 2.6 kb mRNA. Only the 6.0 kb sequence correlated with the receptor concentration determined by 125I-T3 displacement analysis.
Santos
A
A
Department of Medicine, University of Minnesota, Minneapolis 55455.
Freake
H C
HC
Rosenberg
M E
ME
Schwartz
H L
HL
Oppenheimer
J H
JH
D000818:Animals; D015152:Blotting, Northern; D002467:Cell Nucleus; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D011919:Rats, Inbred Strains; D011988:Receptors, Thyroid Hormone / Q000032:analysis*
D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D011988:Receptors, Thyroid Hormone; D012313:RNA