Epitope mapping of an HLA-DR-specific monoclonal antibody produced by using human-mouse transfectant cells.
A polymorphic HLA-DR mAb, TAL15.1, was produced against L cells transfected with DR alpha- and beta-chain cDNA from a cell line homozygous for HLA-DRw8. This antibody reacted with DRw8 plus all other DR types except DR3 and DRw52. DR3 and DRw52 differ uniquely from other other DR antigens at position 77 in the beta 1-domain of their beta-chains where there is asparagine instead of threonine. In Western blots the antibody reacted with DR alpha/beta-dimer but not with free alpha- or beta-chains. Two-dimensional gel analysis of a DRw11, DRw52 cell line showed that TAL15.1 immunoprecipitated the DR products. Although it also coprecipitated the DQ beta chain products, flow microfluorimetric analysis with various transfectant cell lines showed that TAL15.1 failed to bind the DQ or DP products tested. We conclude that TAL15.1 is a DR-specific polymorphic antibody whose activity correlates with a specific residue. It has already proved to be a valuable reagent for distinguishing DR3 homozygotes from DR3, DRw6 heterozygotes, which have in the past been difficult to separate.
Madrigal
J A
JA
Imperial Cancer Research Fund, London, United Kingdom.
Ikeda
H
H
Marsh
S G
SG
Heyes
J M
JM
Kennedy
L J
LJ
Trowsdale
J
J
Bodmer
J G
JG
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000096:biosynthesis; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D002469:Cell Separation; D002999:Clone Cells / Q000276:immunology; D000939:Epitopes / Q000032:analysis / Q000276:immunology*; D006684:HLA-DR Antigens / Q000032:analysis / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007739:L Cells; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D014162:Transfection
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006684:HLA-DR Antigens