title: Acute-phase proteins and the serological evaluation of experimental contact sensitivity in the mouse.
abstract: The evaluation of contact reactions in previously sensitized mice is assessed conventionally by measurement of increases in ear thickness following challenge. In an attempt to develop a serological method for the investigation of contact sensitization in mice, we have examined whether analysis of changes in the concentration of acute-phase proteins in response to challenge provides a reliable alternative means of evaluating elicitation reactions. Measurement of either the relative serum haptoglobin concentration, using radial immunodiffusion, or the absolute concentration of serum amyloid A, by an enzyme-linked immunosorbent assay, has been found to correlate well with induced increases in ear thickness following challenge. Changes in the concentration of acute-phase proteins proved to be of sufficient sensitivity to reflect the specificity of contact sensitization and its inhibition by antigenic competition.
authors: ICI Central Toxicology Laboratory, Macclesfield, UK.
authors: I
authors: I
authors: Kimber
authors: R K
authors: RK
authors: Ward
authors: C J
authors: CJ
authors: Shepherd
authors: M N
authors: MN
authors: Smith
authors: K P
authors: KP
authors: McAdam
authors: J G
authors: JG
authors: Raynes
mesh_terms: D000209:Acute-Phase Proteins / Q000097:blood*; D000818:Animals; D003877:Dermatitis, Contact / Q000097:blood* / Q000473:pathology; D006242:Haptoglobins / Q000097:blood*; D051379:Mice; D008807:Mice, Inbred BALB C; D010081:Oxazolone / Q000097:blood; D000685:Serum Amyloid A Protein / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000209:Acute-Phase Proteins; D006242:Haptoglobins; D000685:Serum Amyloid A Protein; D010081:Oxazolone