Detection of gold probes with video-enhanced contrast microscopy: nanovid microscopy.
This contribution describes a new microscopic technique aimed at visualizing colloidal gold particles of 20-40 nm diameter as dynamic markers at the light microscopic level. The technique, called nanovid microscopy, is based on the use of contrast enhancement by video techniques and digital image processing. Two applications on living cells are discussed. The first is the receptor-mediated endocytosis of the transferrin receptor, which for the first time can be followed in real time. A second application documents the motion of cell-surface glycoproteins on PTK2-cells. In addition, nanovid microscopy allows the number of gold particles in immunogold staining to be quantified easily. With this tool, the translocation of specific molecules in living cells becomes one of the most enjoyable activities to observe.
De Brabander
M
M
Department of Life Sciences, Janssen Research Foundation, Beerse, Belgium.
Geerts
H
H
Nuydens
R
R
Nuyens
R
R
D001692:Biological Transport; D004705:Endocytosis; D005404:Fixatives; D006046:Gold / Q000493:pharmacokinetics; D007089:Image Enhancement; D007425:Intracellular Membranes / Q000378:metabolism; D008845:Microinjections; D008853:Microscopy / Q000379:methods*; D010316:Particle Size; D011956:Receptors, Cell Surface / Q000502:physiology; D013194:Staining and Labeling; D013690:Television
D005404:Fixatives; D011956:Receptors, Cell Surface; D006046:Gold