Sulfated glycoproteins synthesized by the corneal epithelium of chick embryo having the same molecular weights as cytokeratin polypeptides.
The previous study from this laboratory demonstrated that the corneal epithelium of 19-d-old chick embryo synthesizes two classes of sulfated glycoconjugates consisting of sulfated glycoproteins and proteoglycans (Yonekura, H., Oguri, K., Nakazawa, K., Shimizu, S., Nakanishi, Y., & Okayama, M. (1982) J. Biol. Chem. 257, 11166-11175). The present study demonstrated that when the sulfated glycoproteins labeled metabolically with [35S]sulfate and [3H]glucosamine were analyzed by SDS-PAGE, the 70,000 component (accounting for approximately 30% of the 35S and 35% of the 3H of the total sulfated glycoprotein) co-migrated with five major proteins with apparent molecular weights (Mrs) of 70,000, 66,000, 58,000, 51,000, and 48,000, which together accounted for about 57% of the total tissue protein. All five proteins cross-reacted with an antibody against human sole keratin, indicating that they are cytokeratin polypeptides of the corneal epithelium. Amino acid analysis demonstrated that they had high contents of glycine, serine, glutamic acid, leucine, and aspartic acid. Two-dimensional tryptic peptide maps indicated that they were all different. Analysis of radiolabeled materials released by alkaline borohydride treatment of the sulfated glycoproteins which were synthesized in the presence and absence of tunicamycin and co-purified with the five cytokeratin polypeptides, revealed that they contained both N- and O-glycosidically linked sulfated oligosaccharides. All the results obtained in the present study indicate that the five sulfated glycoproteins are similar, if not identical, to the cytokeratin polypeptides. This is consistent with the result in the accompanying paper that these sulfated glycoproteins are localized intracellularly.
Yonekura
H
H
Clinical Research Institute, National Nagoya Hospital, Aichi.
Oguri
K
K
Itoh
K
K
Tanabe
K
K
Takahashi
N
N
Nakanishi
Y
Y
Okayama
M
M
D000818:Animals; D002642:Chick Embryo; D051152:Clusterin; D003315:Cornea / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004848:Epithelium / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis*; D007633:Keratins; D018832:Molecular Chaperones; D008970:Molecular Weight; D010449:Peptide Mapping; D011509:Proteoglycans / Q000096:biosynthesis*; D012967:Sodium Dodecyl Sulfate; D013462:Sulfur Radioisotopes; D014316:Tritium
D051152:Clusterin; D006023:Glycoproteins; D018832:Molecular Chaperones; D011509:Proteoglycans; D013462:Sulfur Radioisotopes; D014316:Tritium; D012967:Sodium Dodecyl Sulfate; D007633:Keratins