Dust from carpeted and smooth floors. II. Antigenic and allergenic content of dust vacuumed from carpeted and smooth floors in schools under routine cleaning schedules.
Dust samples were collected twice from smooth and carpeted floors in 10 Norwegian schools. The content of antigens and allergens of alder (Alnus incana), birch (Betula verrucosa), timothy (Phleum pratense), cat and dog dander, house dust mite (Dermatophagoides farinae), mould (Cladosporium herbarum), hen egg white and codfish (DIII) were investigated by crossed immunoelectrophoresis (CIE), crossed radio immunoelectrophoresis (CRIE), radio allergosorbent test (RAST) inhibition and quantitative precipitation inhibition analysis by laser nephelometry. Antigens and allergens of cat and dog dander and hen egg white were most prevalent in the dust samples investigated. With the exception of hen egg white and codfish allergens, no statistically significant differences in mean allergen content were shown in identical quantities of freeze-dried dust extracts from carpeted and smooth floors. RAST-inhibition analyses of identical amounts of dust from either floors showed higher content of allergens of cat, dog, hen egg white, codfish, mould and timothy pollen in classrooms with carpets.
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Laboratory of Clinical Biochemistry, University Hospital, Bergen, Norway.
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Elsayed
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D000485:Allergens / Q000032:analysis*; D000941:Antigens / Q000032:analysis*; D001249:Asthma / Q000517:prevention & control*; D002648:Child; D004391:Dust / Q000032:analysis*; D000939:Epitopes / Q000032:analysis; D005160:Facility Design and Construction; D005430:Floors and Floorcoverings; D006796:Household Work / Q000379:methods*; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D008970:Molecular Weight; D011852:Radioallergosorbent Test; D012130:Respiratory Hypersensitivity / Q000517:prevention & control*; D012574:Schools
D000485:Allergens; D000941:Antigens; D004391:Dust; D000939:Epitopes