A lymphocyte molecule implicated in lymph node homing is a member of the cartilage link protein family.
Monoclonal antibodies in the Hermes family recognize a lymphocyte structure that participates in lymphocyte adhesion to endothelium and has been suggested to be the human homolog of the murine Mel-14 lymph node homing receptor. Recently, antibodies against the Hermes antigen, the polymorphic glycoprotein Pgp-1 antigen, and the broadly expressed CDw44 antigen have been shown to recognize the same structure. In this work, cDNA clones encoding the CDw44 antigen were isolated and expressed in COS cells. Two forms were identified: a lymphoid form expressed in hematopoietic cells, and an epithelial form weakly expressed in normal epithelium but highly expressed in carcinomas. The extracellular domain of CDw44 bears homology to cartilage link proteins and a related segment of proteoglycan core protein. However, comparison with the recently identified sequence of the Mel-14 antigen shows that CDw44 and Mel-14 are unrelated.
Stamenkovic
I
I
Department of Pathology, Harvard Medical School, Massachusetts General Hospital, Boston 02114.
Amiot
M
M
Pesando
J M
JM
Seed
B
B
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000032:analysis / Q000302:isolation & purification; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D006801:Humans; D008198:Lymph Nodes / Q000502:physiology*; D008214:Lymphocytes / Q000032:analysis*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011506:Proteins / Q000032:analysis*; D011509:Proteoglycans; D012313:RNA / Q000032:analysis; D011971:Receptors, Immunologic / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D016201:Receptors, Lymphocyte Homing; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured; D000099249:Proteoglycan Link Protein
D004247:DNA; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; D011506:Proteins; D011509:Proteoglycans; D012313:RNA; D011971:Receptors, Immunologic; D016201:Receptors, Lymphocyte Homing; D000099249:Proteoglycan Link Protein