title: Characterization of human marrow stromal cells: role in progenitor cell binding and granulopoiesis.
abstract: Adherent cell layers and their associated extracellular matrices form when human marrow is incubated in cultures containing hydrocortisone and horse serum. These stromal layers contain cells positive for alkaline phosphatase; secrete collagens types I and III and fibronectin, bind the anti-actin monoclonal antibodies (MoAbs) HHF and CGA-7; stain with oil red O, and express the acetylated LDL receptor. Highly purified CD34 (My10)-positive progenitor cells attach to these stromal layers, and a 16-fold enrichment of CFU-GM in both stromal attachment and semisolid agar assays was observed. Granulopoiesis persisted up to 40 days (mean duration 25 days) after passaged stroma were recharged with stromal cell-depleted target cells in a two-stage liquid marrow culture system. Although equal to marrow fibroblasts in their ability to bind CD34+ myeloid progenitors, stromal layers were better at supporting granulopoiesis. This system provides an in vitro model to characterize the components of stroma and stroma-cytomatrix that enhance marrow progenitor cell localization and maintenance.
authors: Department of Medicine, University of Rochester School of Medicine, NY.
authors: J L
authors: JL
authors: Liesveld
authors: C N
authors: CN
authors: Abboud
authors: R E
authors: RE
authors: Duerst
authors: D H
authors: DH
authors: Ryan
authors: J K
authors: JK
authors: Brennan
authors: M A
authors: MA
authors: Lichtman
mesh_terms: D018952:Antigens, CD34; D000943:Antigens, Differentiation; D001854:Bone Marrow Cells; D002448:Cell Adhesion; D002451:Cell Compartmentation; D002470:Cell Survival; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000502:physiology*; D005347:Fibroblasts / Q000502:physiology; D006098:Granulocytes / Q000502:physiology*; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000145:classification / Q000502:physiology; D006801:Humans; D010641:Phenotype; D013234:Stem Cells / Q000502:physiology*
chemical_list: D018952:Antigens, CD34; D000943:Antigens, Differentiation