Antibodies affect the ionic conductance of channels formed by amphotericin B in a lipid bilayer.
For the first time poly- and monoclonal antibodies (class IgM) against the polyene antibiotic amphotericin B were obtained affecting the properties of a channel formed by the antibiotic and cholesterol in a lipid bilayer when amphotericin B was added to the solution at one (cis) side of the membrane. In the case of the symmetric distribution of cholesterol in the lipid bilayer, three molecules of monoclonal antibodies bind firmly to the channel at the trans-side of the membrane, thus strongly increasing the mean lifetime of the channel in the open state, and not changing practically the ion conductance of its open state. The antibodies did not alter the properties of these channels when added at the cis-side of the membrane as well as of the channels formed in the lipid bilayer when amphotericin B was added at both membrane sides. The antibodies obtained did not affect the conductance of channels in which amphotericin B and cholesterol were replaced with their analogs levorin and 5 alpha-androstan-3 beta-one, which points to a high specificity of the immunoglobulins isolated. When cholesterol was present only in the cis-monolayer of the lipid bilayer and was absent in the trans-monolayer, the same monoclonal antibodies when added at the trans-side of the membrane blocked the conductance of the channel formed by adding the antibiotic to the solution at the cis-side of the bilayer. The obtained evidence is of interest in elucidating the general features of interaction of antibodies with the ionic channels of cellular and model membranes.
Kolomytkin
O V
OV
Institute of Biological Physics, Academy of Sciences of the USSR, Pushchino.
Mantzyghin
J A
JA
Swyatukhina
N V
NV
D000666:Amphotericin B / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex; D000937:Antigen-Antibody Reactions; D002784:Cholesterol / Q000502:physiology; D004306:Dose-Response Relationship, Immunologic; D004553:Electric Conductivity; D004875:Ergosterol / Q000502:physiology; D007132:Immunoglobulin Isotypes / Q000276:immunology; D007473:Ion Channels / Q000276:immunology* / Q000502:physiology / Q000648:ultrastructure; D008051:Lipid Bilayers; D008563:Membrane Lipids / Q000502:physiology; D011090:Polyenes; D013757:Tetraethylammonium Compounds / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D007132:Immunoglobulin Isotypes; D007473:Ion Channels; D008051:Lipid Bilayers; D008563:Membrane Lipids; D011090:Polyenes; D013757:Tetraethylammonium Compounds; D000666:Amphotericin B; D002784:Cholesterol; D004875:Ergosterol