[Isolation and study of polyadenylated RNA from Bacillus intermedius].
A poly(A)+RNA fraction was isolated from the overall RNA of Bacillus intermedius using chromatography on poly(U) Sepharose and was shown to be electrophoretically heterogeneous. The presence of a polyadenylate segment was confirmed by hybridization with polyuridine. The biological activity of the poly(A)+RNA was proved by the translation in Xenopus laevis oocytes. The dynamics of poly(A)+RNA synthesis was studied in the course of B. intermedius growth and the content of poly(A)+RNA was assayed in the cells grown in different media.
Romakhina
E R
ER
Bagaeva
T V
TV
Sharipova
F R
FR
Kozyreva
E A
EA
Leshchinskaia
I B
IB
D000818:Animals; D001407:Bacillus / Q000254:growth & development* / Q000378:metabolism; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D009693:Nucleic Acid Hybridization; D009865:Oocytes / Q000378:metabolism; D011061:Poly A / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification*; D011072:Poly U / Q000235:genetics; D014176:Protein Biosynthesis; D012313:RNA / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification*; D012329:RNA, Bacterial / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification*; D014982:Xenopus laevis
D012329:RNA, Bacterial; D012333:RNA, Messenger; D011061:Poly A; D011072:Poly U; D012313:RNA
Vydelenie i izuchenie poliadenilirovannoĭ RNK iz Bacillus intermedius.