Cytogenetic analysis of cell lines derived from metastases of a mammary carcinoma in a dog.
Three cell lines derived from two metastases of a mammary carcinoma in a female dog were analyzed cytogenetically. All three cell lines showed a modal chromosome number of 76, with ranges of 74-77, 72-78, and 73-78. A biarmed chromosome in addition to the X chromosomes was observed in all cells of one cell line, and in a part of the cells of the other two cell lines. Results of banding analyses indicated that this chromosome was identical in the three cell lines, and can thus be considered a clonal marker. Additional biarmed chromosomes have not been reported previously from mammary tumor cells, although their presence is rather common in other canine neoplasms.
Mellink
C H
CH
Department of Functional Morphology, Faculty of Veterinary Sciences, Utrecht, The Netherlands.
Bosma
A A
AA
Rutteman
G R
GR
D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D004285:Dogs; D005260:Female; D007621:Karyotyping; D015674:Mammary Neoplasms, Animal / Q000235:genetics* / Q000473:pathology; D009362:Neoplasm Metastasis; D013194:Staining and Labeling