Isolation and characterization of a cDNA clone coding for human epidermal keratin K5. Sequence of the carboxyterminal half of this keratin.
A cDNA clone encoding human epidermal keratin No. 5 (K5) has been isolated from a lambda gt10 cDNA library on the basis of crosshybridization with cDNAs coding for other basic keratins and differential hybridization with total cDNA probes. This clone contains a 1100-bp insert able to inhibit specifically translation of K5 in a hybrid-arrested translation test. Northern blots show that this insert hybridizes with a 2.4-kb message present in epidermal mRNAs. The 1100-bp sequence reported here corresponds to the 3' half of the K5 message. Comparison of the deduced aminoacid sequence shows that the protein exhibits characteristic features of a basic keratin. The 242 aminoacid sequence reported here extends from the carboxyterminal end up to the last helical portion of the central rod domain.
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Centre International de Recherches Dermatologiques, Sophia Antipolis, Valbonne, France.
Darmon
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D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D004817:Epidermis / Q000378:metabolism*; D005815:Genetic Code; D006801:Humans; D007633:Keratins / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics
D012333:RNA, Messenger; D007633:Keratins; D004247:DNA