rRNA gene restriction patterns of Haemophilus influenzae biogroup aegyptius strains associated with Brazilian purpuric fever.
The rRNA gene restriction patterns of 92 isolates of Haemophilus influenzae biogroup aegyptius, associated with conjunctivitis or Brazilian purpuric fever in the State of São Paulo, Brazil, were studied with 16 + 23S rRNA from Escherichia coli as a probe. All strains were classified into 15 patterns. Isolates from Brazilian purpuric fever cases were seen only in patterns 3 (most frequently) and 4 (rarely), whereas isolates from conjunctivitis were found in all 15 patterns. The study demonstrated that rRNA from E. coli can serve as a probe for molecular epidemiology.
Irino
K
K
Unité 199 Institut National de la Santé et de la Recherche Médicale, Institut Pasteur, Paris, France.
Grimont
F
F
Casin
I
I
Grimont
P A
PA
D001345:Autoradiography; D001938:Brazil; D002648:Child; D003234:Conjunctivitis, Bacterial / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D015246:Deoxyribonuclease EcoRI; D006192:Haemophilus Infections / Q000382:microbiology*; D006193:Haemophilus influenzae / Q000145:classification / Q000235:genetics*; D006801:Humans; D011693:Purpura / Q000382:microbiology*; D012329:RNA, Bacterial / Q000032:analysis / Q000235:genetics; D012335:RNA, Ribosomal / Q000032:analysis / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D015246:Deoxyribonuclease EcoRI