Characterization of cross-reactive blood-stage antigens of the Plasmodium cynomolgi complex using anti-Plasmodium vivax monoclonal antibodies.
Five out of 18 monoclonal antibodies (moAB's) produced against blood stages of a Brazilian (Belem) strain of Plasmodium vivax were shown to cross-react with all of the 11 strains of the P. cynomolgi complex that were assayed. The 5 moAB's produced 3 different patterns of immunofluorescence, identical for both P. vivax and P. cynomolgi. Three of these moAB's appeared to react with antigens associated with the cytoplasm or membranes of infected erythrocytes. By Western blot analysis, 2 of these 3 moAB's identified an antigen with an apparent molecular weight of 31 kDa in extracts of parasitized erythrocytes of both species; the third of these moAB's reacted with an antigen with an apparent molecular weight of 95 kDa. By immunofluorescence, the 2 other moAB's reacted only with parasites at all developmental stages. The target antigen of these 2 moAB's was not identified. Immunoradiometric assays indicated that the moAB's are directed against 3 or possibly 4 distinct nonrepetitive epitopes. None of the moAB's inhibited merozoite invasion or growth of the parasites in an in vitro culture system of the Berok strain of P. cynomolgi.
Kamboj
K K
KK
Department of Medical and Molecular Parasitology, New York University School of Medicine, New York 10010.
Barnwell
J W
JW
Nussenzweig
R S
RS
Cochrane
A H
AH
D000818:Animals; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000032:analysis*; D005075:Biological Evolution; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis; D005455:Fluorescent Antibody Technique; D007158:Immunologic Techniques; D008253:Macaca mulatta; D051379:Mice; D008807:Mice, Inbred BALB C; D010961:Plasmodium / Q000145:classification / Q000276:immunology*; D010966:Plasmodium vivax / Q000276:immunology
D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D000939:Epitopes