title: Anti-idiotypic antibodies that protect cells against the action of diphtheria toxin.
abstract: An anti-idiotypic serum prepared against the combining site (idiotype) of specific anti-diphtheria toxoid antibodies was characterized with respect to its interaction with highly diphtheria toxin-sensitive Vero cells. Although the anti-idiotypic serum protected Vero cells against the cytotoxic action of diphtheria toxin, it did not prevent the binding of 125I-labeled diphtheria toxin to the cells but did inhibit the internalization and degradation of 125I-labeled toxin. This anti-idiotypic serum immunoprecipitated a cell-surface protein from radiolabeled Vero cells with an apparent Mr of approximately 15,000. These results are consistent with the hypothesis that the anti-idiotypic serum contains antibodies that carry an internal image of an internalization site on the toxin and that a cell-surface protein involved in toxin internalization possesses a complementary site recognized by both the toxin and the anti-idiotypic antibodies.
authors: Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235.
authors: J M
authors: JM
authors: Rolf
authors: H M
authors: HM
authors: Gaudin
authors: S M
authors: SM
authors: Tirrell
authors: A B
authors: AB
authors: MacDonald
authors: L
authors: L
authors: Eidels
mesh_terms: D000818:Animals; D002470:Cell Survival; D004167:Diphtheria Toxin / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D066257:Heparin-binding EGF-like Growth Factor; D007106:Immune Sera / Q000494:pharmacology*; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007163:Immunosorbent Techniques; D036341:Intercellular Signaling Peptides and Proteins; D007457:Iodine Radioisotopes; D008970:Molecular Weight; D011956:Receptors, Cell Surface; D011950:Receptors, Cholinergic / Q000032:analysis / Q000276:immunology; D014709:Vero Cells
chemical_list: D004167:Diphtheria Toxin; D000939:Epitopes; D066257:Heparin-binding EGF-like Growth Factor; D007106:Immune Sera; D007130:Immunoglobulin Idiotypes; D036341:Intercellular Signaling Peptides and Proteins; D007457:Iodine Radioisotopes; D011956:Receptors, Cell Surface; D011950:Receptors, Cholinergic