Localization and purification of SBU-4--a pregnancy specific protein of the ovine uterus.
In order to elucidate the function of molecules of the ovine maternal-fetal interface a monoclonal antibody was produced to intact interplacentomal trophoblast membranes. Extensive immunohistological studies revealed that the monoclonal antibody recognizes a protein designated SBU-4 which originates in the intercaruncular regions of the gravid sheep uterus at about the time of implantation and increases in concentration throughout gestation. The data suggest that SBU-4 is produced by endometrial epithelial cells and that adjacent uninucleate cells of the trophoblast acquire the antigen by endocytosis. Initial biochemical analysis of the purified SBU-4 molecule prepared by monoclonal antibody immunoaffinity chromatography indicates that SBU-4 is high molecular weight glycoprotein complex comprising several sub-units.
Mercer
W R
WR
Department of Veterinary Preclinical Sciences, University of Melbourne, Parkville, Victoria, Australia.
Gogolin-Ewens
K J
KJ
Lee
C S
CS
Brandon
M R
MR
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002846:Chromatography, Affinity; D000939:Epitopes; D005260:Female; D006649:Histocompatibility Antigens / Q000276:immunology / Q000302:isolation & purification*; D015395:Histocompatibility Antigens Class I; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D008970:Molecular Weight; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000276:immunology / Q000302:isolation & purification*; D012756:Sheep / Q000502:physiology*; D014599:Uterus / Q000032:analysis / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006649:Histocompatibility Antigens; D015395:Histocompatibility Antigens Class I; D011257:Pregnancy Proteins; C025625:pregnancy-specific antigen, sheep