Amino acid sequence of a conserved neutralizing epitope of murine coronaviruses.
We identified the binding site of monoclonal antibody 19.2, which cross-neutralizes several mouse hepatitis virus (MHV) strains, inhibits fusion of MHV-infected cells, and protects against lethal infection (P. J. Talbot and M. J. Buchmeier, Virus Res. 2:317-328, 1985). We used fusion proteins, generated by expression of fragments of the MHV A59 E2 gene in pEX plasmids, and synthetic peptides in a PEPSCAN.
Luytjes
W
W
Institute of Virology, Veterinary Faculty, University of Utrecht, The Netherlands.
Geerts
D
D
Posthumus
W
W
Meloen
R
R
Spaan
W
W
D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D000939:Epitopes; D008969:Molecular Sequence Data; D006517:Murine hepatitis virus / Q000235:genetics* / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics*
D000956:Antigens, Viral; D000939:Epitopes; D014759:Viral Envelope Proteins