[The development of methods for obtaining monospecific ingredients for immunoenzyme analysis].
The methods of the modification of Salmonella O- and H-antigens and the preparation of biologically active sorbents on their basis have been developed. The use of these sorbents has permitted the isolation of affinity antibodies with strictly defined specific activity. The work shows the possibility of the successful use of carriers obtained on the basis of porous glass, chemically modified by acrylic copolymers containing activated carboxylic groups, and intended for the immobilization of antigens of both protein and carbohydrate nature.
Vaneeva
L I
LI
Iankina
N F
NF
Ivanov
A E
AE
Razguliaeva
O A
OA
Zubov
V P
VP
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D000915:Antibody Affinity; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D000939:Epitopes / Q000032:analysis / Q000302:isolation & purification*; D010861:Fimbriae, Bacterial / Q000276:immunology; D007124:Immunoenzyme Techniques; D007163:Immunosorbent Techniques; D019081:O Antigens; D011817:Rabbits; D012475:Salmonella / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000939:Epitopes; C022863:H antigen; D019081:O Antigens
Razrabotka metodov polucheniia monospetsificheskikh ingredientov dlia immunofermentnogo analiza.