Efficient tissue-specific expression of bovine alpha-lactalbumin in transgenic mice.
Transgenic mice carrying a 3.2-kb genomic fragment comprising the bovine alpha-lactalbumin gene transcription unit with 750 bp and 336 bp of 5' and 3' flanking regions, respectively, were generated. Tissue-specific expression of the transgene was observed in five out of the six lines analyzed at levels close to the endogenous gene in two of them.
Vilotte
J L
JL
Laboratoire de Génétique Biochimique, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
Soulier
S
S
Stinnakre
M G
MG
Massoud
M
M
Mercier
J C
JC
D000818:Animals; D015153:Blotting, Western; D005700:Galactosyltransferases / Q000378:metabolism; D007768:Lactalbumin / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics* / Q000378:metabolism; D008892:Milk / Q000032:analysis; D012313:RNA / Q000032:analysis*; D014158:Transcription, Genetic
D012313:RNA; D007768:Lactalbumin; D005700:Galactosyltransferases