C-reactive protein reacts with the U1 small nuclear ribonucleoprotein.
C-reactive protein (CRP) was found to produce a small, discrete, speckled fluorescence pattern in the nucleus of HEp-2 cells. Double staining with anti-RNP serum and CRP produced very similar staining patterns. By counterimmunoelectrophoresis CRP was bound to extractable nuclear antigens found in rabbit thymus extract. The reactive components of the extract were only partially sensitive to treatment with RNase. CRP immunoprecipitated the U1 RNA species from [32P]labeled HeLa cells and the protein bands of the Sm/RNP complex from [35S]-methionine-labeled HeLa cells. By blotting, CRP bound to several discrete bands in a calcium-dependent, PC-inhibitable manner. Two of the bands comigrated with the 70K protein band associated with the U1 snRNP, and its major breakdown product. Binding to these bands was inhibited by both EDTA and PC indicating that CRP binds these proteins through the PC-binding site. Binding to the 70K protein of the U1 snRNP was confirmed by reactivity with the recombinant 70K protein in a dot blot. These findings indicate the CRP binds to the U1-RNP snRNP particle. Considering the ability of CRP to inhibit antibody responses to its ligands and its ability to activate C and promote phagocytosis it is suggested that CRP may play a role in the regulation of autoantibody responses to nuclear Ag.
Du Clos
T W
TW
Department of Medicine, VA Medical Center, Albuquerque, New Mexico 87108.
D000937:Antigen-Antibody Reactions; D034961:Antigens, Nuclear; D001324:Autoantigens / Q000276:immunology; D001666:Binding Sites, Antibody; D002097:C-Reactive Protein / Q000276:immunology* / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D007123:Immunoelectrophoresis, Two-Dimensional; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000276:immunology; D011233:Precipitin Tests; D012261:Ribonucleoproteins / Q000276:immunology* / Q000378:metabolism; D017411:Ribonucleoproteins, Small Nuclear; D013194:Staining and Labeling; D013462:Sulfur Radioisotopes
D034961:Antigens, Nuclear; D001324:Autoantigens; D009687:Nuclear Proteins; D012261:Ribonucleoproteins; D017411:Ribonucleoproteins, Small Nuclear; D013462:Sulfur Radioisotopes; D002097:C-Reactive Protein; D008715:Methionine