Differentiation of endocrine cells in chick allantoic epithelium combined with pancreatic mesenchyme.
Allantoic endoderm of 3-day chick embryos was combined with pancreatic mesenchyme of 5-day embryos and cultured as chorio-allantoic grafts for a total of 14 days. Recombinations of endoderm and mesenchyme of the pancreas and of the allantois served as controls. The usual types of endocrine cells differentiated in the pancreatic controls, none in the allantoic controls. In experimental grafts simple columnar epithelium with goblet cells and a sucrase-positive brush border developed; a few insulin cells and endocrine cells typical of the intestine differentiated. Hence allantoic endoderm has endocrine potentiality not realised in vivo, where its own mesenchyme may be inhibitory.
Stein
B
B
Department of Anatomy, Medical School, University of the Witwatersrand, Johannesburg, South Africa.
Andrew
A
A
D000482:Allantois / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D002642:Chick Embryo; D004702:Endocrine Glands / Q000166:cytology* / Q000378:metabolism; D004707:Endoderm / Q000166:cytology / Q000378:metabolism; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005321:Extraembryonic Membranes / Q000166:cytology*; D005755:Gastrins / Q000378:metabolism; D005934:Glucagon / Q000378:metabolism; D007150:Immunohistochemistry; D007328:Insulin / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology; D008648:Mesoderm / Q000166:cytology* / Q000378:metabolism; D009037:Motilin / Q000378:metabolism; D009496:Neurotensin / Q000378:metabolism; D010179:Pancreas / Q000166:cytology* / Q000378:metabolism; D010191:Pancreatic Polypeptide / Q000378:metabolism; D012633:Secretin / Q000378:metabolism; D013004:Somatostatin / Q000378:metabolism; D013373:Substance P / Q000378:metabolism; D014183:Transplantation, Heterologous
D005755:Gastrins; D007328:Insulin; D012633:Secretin; D013373:Substance P; D009496:Neurotensin; D013004:Somatostatin; D009037:Motilin; D010191:Pancreatic Polypeptide; D005934:Glucagon