title: Acetylcholine-gated and chloride conductance channel expression in rat muscle membrane.
abstract: 1. During the differentiation of skeletal muscle, there is a synchronized expression of a number of muscle-specific proteins including the acetylcholine-gated ion channel (AChR). Another muscle-specific ion channel, responsible for chloride conductance, was shown to be expressed in an anticoordinate fashion to AChR. An organ culture system for rat lumbrical muscles was developed to manipulate the expression of these two ion channels. 2. Denervation induced a change in expression of both channels that was mimicked in culture and reversed by direct electrical stimulation. 3. The time course of the disappearance of both channels was similar and started immediately after denervation (chloride conductance) or stimulation (AChR). The time course of the appearance of AChR was delayed several days after denervation and culture but chloride conductance increased immediately upon stimulation. 4. The loss of chloride conductance in muscle cultured in cycloheximide exhibited first-order kinetics, providing an estimate of the half-life (2.3 days) for the chloride conductance channel. This resembled the disappearance of chloride conductance in normal medium, suggesting that synthesis of this channel ceases following denervation. The decrease in chloride conductance characteristic of denervated muscle was not halted by cycloheximide. 5. Changes in chloride conductance presumably alter the intracellular concentration of chloride. The possibility that chloride might regulate the expression of AChRs in skeletal muscle was tested by altering the intracellular concentration of chloride in muscles maintained in organ culture. 6. Denervated muscles, whose intracellular concentration of chloride is elevated, were cultured in medium containing 9 mM-chloride (low-Cl- medium). AChR expression was reduced by either low-Cl- medium or electrical stimulation. Together, low-Cl- medium and electrical stimulation reduced expression more than either treatment alone. 7. The loss of AChRs in low-Cl- medium was blocked when muscle fibrillation was halted by TTX. 8. When chloride conductance was blocked by 9AC (9-anthracene carboxylic acid) intracellular chloride was elevated to the levels seen in denervated muscle. The elevated levels of chloride did not prevent the reduction in AChR expression induced by electrical stimulation. 9. The uncoupling of AChR expression and the intracellular concentration of chloride showed that they were not rigidly linked. Chloride affects the expression of AChR indirectly, by altering the activity of muscle cells.
authors: Department of Physiology, University of Colorado School of Medicine, Denver 80206.
authors: R D
authors: RD
authors: Heathcote
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002712:Chlorides / Q000378:metabolism* / Q000494:pharmacology / Q000502:physiology; D003513:Cycloheximide / Q000494:pharmacology; D004553:Electric Conductivity; D004558:Electric Stimulation; D015640:Ion Channel Gating; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D008566:Membranes / Q000378:metabolism; D009132:Muscles / Q000378:metabolism*; D009924:Organ Culture Techniques; D051381:Rats; D011950:Receptors, Cholinergic / Q000378:metabolism
chemical_list: D002712:Chlorides; D007473:Ion Channels; D011950:Receptors, Cholinergic; D000001:Calcimycin; D003513:Cycloheximide; D000109:Acetylcholine