Sequence organization of the human int-2 gene and its expression in teratocarcinoma cells.
We report the genomic organization and DNA sequence of the human homologue of int-2, a proto-oncogene implicated in virally induced mammary tumours in the mouse, and expressed at specific sites and times during embryogenesis. Direct comparisons with the coding domains of mouse int-2 allowed us to delineate the intron-exon boundaries of the human gene. These boundaries were subsequently confirmed by ribonuclease protection analyses of the single 1.7 kilobase (kb) int-2 transcript detectable in the human teratocarcinoma cell line, Tera-2. The data suggest that human int-2 may also function in embryonic lineages but that its transcription may be less complex than in the mouse. The predicted human protein comprises 239 amino acids and is 89% homologous to its murine counterpart, except at the carboxy terminus. This divergence occurs distal to the region of int-2 that shows homology to other members of the FGF family of growth modulators and oncogenes.
Brookes
S
S
Imperial Cancer Research Fund Laboratories, St. Bartholomew's Hospital, London, UK.
Smith
R
R
Casey
G
G
Dickson
C
C
Peters
G
G
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D004247:DNA / Q000032:analysis; D005346:Fibroblast Growth Factors / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes; D012313:RNA / Q000032:analysis; D012260:Ribonucleases / Q000494:pharmacology; D013724:Teratoma / Q000235:genetics*; D014158:Transcription, Genetic
C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D005346:Fibroblast Growth Factors; D012313:RNA; D004247:DNA; D012260:Ribonucleases