Purification, characterization, and localization of neuropeptides in the cornea.
The immunologically detected neuropeptides methionine enkephalin (ME), substance P (SP), beta-endorphin (beta-End), and alpha-melanocyte stimulating hormone (alpha-MSH) were purified from bovine corneal extracts by gradient, followed by isocratic, reversed phase-high performance liquid chromatography (RP-HPLC) and characterized, after both chromatographic steps, by radioimmunoassay (RIA). Immunologically detected ME and SP were purified from canine corneal extracts by gradient RP-HPLC and characterized by RIA. An anatomical study of the bovine cornea separated the cornea into an epithelium-enriched and a stroma-enriched portion. After gradient RP-HPLC, RIA demonstrated that all the ME-like immunoreactivity was located in the corneal epithelium, whereas the SP-like immunoreactivity was distributed between the stroma and epithelium in an approximate two-to-one ratio.
Tinsley
P W
PW
Charles B. Stout Neuroscience Mass Spectrometry Laboratory, University of Tennessee, Memphis 38163.
Fridland
G H
GH
Killmar
J T
JT
Desiderio
D M
DM
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003315:Cornea / Q000032:analysis* / Q000166:cytology; D004285:Dogs; D004744:Enkephalin, Methionine / Q000032:analysis; D009479:Neuropeptides / Q000032:analysis* / Q000302:isolation & purification; D011863:Radioimmunoassay; D013373:Substance P / Q000032:analysis; D000521:alpha-MSH / Q000032:analysis; D001615:beta-Endorphin / Q000032:analysis
D009479:Neuropeptides; D013373:Substance P; D000521:alpha-MSH; D004744:Enkephalin, Methionine; D001615:beta-Endorphin