[Highly sensitive determination of the dehydrogenase activity in human peripheral blood lymphocytes using a bioluminescent method].
A highly sensitive rapid method for measuring human blood lymphocyte NADP-dependent dehydrogenase activities has been developed, based on biofluorescent assay of NADPH in a sample with the use of fluorescent bacterial system. The enzyme activity in a suspension of destroyed lymphocytes is determined from NADPH production after addition of appropriate substrates and cofactors. The technique takes little initial material (at least 1000 lymphocytes per sample) and permits 100 measurements in 3 hours.
Savchenko
A A
AA
Suntsova
L N
LN
D006801:Humans; D008163:Luminescent Measurements; D008214:Lymphocytes / Q000201:enzymology*; D008722:Methods; D009247:NADH, NADPH Oxidoreductases / Q000097:blood*; D009252:NADPH Dehydrogenase / Q000097:blood*
D009247:NADH, NADPH Oxidoreductases; D009252:NADPH Dehydrogenase
Vysokochuvstvitel'noe opredelenie aktivnosti degidrogenaz v limfotsitakh perifericheskoĭ krovi cheloveka bioliuminestsentnym metodom.