Fluorography of tritium-labeled proteins in silver-stained polyacrylamide gels.
Silver-staining of polyacrylamide gel electrophoresis (PAGE)-separated proteins allows sensitive detection of proteins but severely reduces the ability to detect weak beta-emitters present in the protein band. A simple procedure is described in which silver can be removed from a silver-stained PAGE gel (deargentation) using photographic fixer, and the silver-free gel can be enhanced and used for fluorography. A quantitative study of sensitivity is reported for 3H-labeled bovine serum albumin with a one-dimensional sodium dodecyl sulfate-PAGE slab gel.
Kulcsár
P
P
Department of Chemistry, State University of New York, Stony Brook 11794-3400.
Prestwich
G D
GD
D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D011506:Proteins / Q000032:analysis*; D012834:Silver; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling; D014316:Tritium
D011506:Proteins; D014316:Tritium; D012834:Silver