Structure of the lipopolysaccharide antigenic O-chain produced by Actinobacillus pleuropneumoniae serotype 4 (ATCC 33 378).
The structure of the antigenic O-polysaccharide part of the S-type lipopolysaccharide produced by Actinobacillus pleuropneumoniae serotype 4 has been determined by periodate oxidation, methylation, partial hydrolysis, and 1H- and 13C-n.m.r. spectroscopy. The O-polysaccharide structure has a branched-tetrasaccharide repeating unit, (----3)-beta-D-Galp-(1----4)-[beta-D-Glcp-(1----3)]-beta-D-GalpNAc- (1----4)-alpha-L-Rhap-(1-)n. The structure resembles that of the lipopolysaccharide O-chain of A. pleuropneumoniae serotype 7, and their common epitopes may account for the apparent serological cross-reactivity observed between the two serotypes when incompletely adsorbed, anticapsular-typing sera are used.
Altman
E
E
Division of Biological Sciences, National Research Council of Canada, Ottawa.
Perry
M B
MB
Brisson
J R
JR
D000188:Actinobacillus / Q000032:analysis*; D000942:Antigens, Bacterial; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D019081:O Antigens; D010084:Oxidation-Reduction; D010504:Periodic Acid
D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D019081:O Antigens; D010504:Periodic Acid; C009288:metaperiodate