Human monoclonal antibodies against cytokeratin 18 generated from patients with gastric cancer.
By co-culturing regional lymph node B-cells and HAT-sensitive mutant cells obtained from RPMI-1788 cells, no less than 20,000 Epstein-Barr (EB)-transformed colonies were obtained from 32 patients with gastric cancer. From B-cell cultures generating antibodies reactive with gastric cancer tissues as well as cultured gastric cancer cells, two EB-transformed cell clones termed C418-59 and C1218-39 were isolated. Both of them produced human IgM-class antibodies, termed Mab418-59 and Mab1218-39, respectively. Both antibodies reacted with an antigen with a molecular weight of 45 kd existing in gastric cancer MKN-45, MKN-1, and Kato-III cells, and also with all of 4 adenocarcinomas of the stomach in paraffin sections. The antigen recognized by both antibodies was identified as a kind of cytoskeletal protein, cytokeratin 18. In this study, it was confirmed that B-cell clones generating autoantibodies against cytokeratin 18 were present in some patients with gastric cancer.
Abe
T
T
Medical Science Laboratory, Asahi Chemical Industry Co., Ltd., Shizuoka.
Fukumoto
M
M
Tsuchiya
K
K
Kuramochi
K
K
Furuta
T
T
Togoh
S
S
Nishiyama
K
K
Tsuchiya
S
S
D000230:Adenocarcinoma / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000941:Antigens / Q000276:immunology; D000951:Antigens, Neoplasm / Q000032:analysis / Q000276:immunology; D001323:Autoantibodies / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D015153:Blotting, Western; D002472:Cell Transformation, Viral; D004591:Electrophoresis, Polyacrylamide Gel; D004854:Herpesvirus 4, Human; D006801:Humans; D007124:Immunoenzyme Techniques; D007075:Immunoglobulin M / Q000276:immunology; D007633:Keratins / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology; D008970:Molecular Weight; D013274:Stomach Neoplasms / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000941:Antigens; D000951:Antigens, Neoplasm; D001323:Autoantibodies; D007075:Immunoglobulin M; D007633:Keratins