Compound 48/80 elicits cholinergic contraction through histamine release in the chick oesophagus.
1. The effects of compound 48/80 on cholinergic nerve transmission and on the liberation of histamine in the isolated chick oesophagus were investigated. 2. Compound 48/80 (0.2-1 microgram ml-1) potentiated the cholinergic nerve-induced contraction and caused the contraction of the oesophagus itself. The latter response was prevented by atropine, tetrodotoxin and mepyramine but not by metiamide. 3. Compound 48/80 released dose-dependently endogenous histamine from the isolated chick oesophagus. 4. These results suggest that the action of compound 48/80 is mediated by the release of histamine from an endogenous pool and that histamine released endogenously facilitates the release of ACh from the intrinsic cholinergic nerve in the chick oesophagus.
Taneike
T
T
Department of Veterinary Pharmacology, Faculty of Dairy Science, Rakuno Gakuen University, Ebetsu, Japan.
Miyazaki
H
H
Oikawa
S
S
Ohga
A
A
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002645:Chickens; D004246:Dimethylphenylpiperazinium Iodide / Q000494:pharmacology; D004558:Electric Stimulation; D004947:Esophagus / Q000187:drug effects / Q000378:metabolism*; D006636:Histamine Release / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D013564:Sympathetic Nervous System / Q000187:drug effects*; D013779:Tetrodotoxin / Q000494:pharmacology; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology*
D003189:p-Methoxy-N-methylphenethylamine; D013779:Tetrodotoxin; D004246:Dimethylphenylpiperazinium Iodide; D001285:Atropine; D000109:Acetylcholine