Effects of calcium ionophore and phorbol ester on class-II-restricted virus-specific human cytotoxic T cell clones.
Calcium ionophore A23187 and phorbol myristate acetate (PMA) were studied for their functional effects on human class-II-restricted measles virus-specific CD4+ cytotoxic T lymphocyte (CTL) clones. A23187 and PMA each dramatically inhibited virus-specific CTL activity. Both PMA and A23187 induced proliferation of resting clones and augmented relatively weak or suboptimal proliferative responses, but inhibited vigorous antigen- or mitogen-induced proliferation. The parallel responses induced by these two agents suggest that they produce their effects via similar mechanisms, most likely through protein kinase C.
Richert
J R
JR
Department of Neurology, Georgetown University Medical Center, Washington, D.C.
Robinson
E D
ED
D000956:Antigens, Viral / Q000276:immunology; D000001:Calcimycin / Q000494:pharmacology*; D002999:Clone Cells / Q000187:drug effects; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D000939:Epitopes / Q000276:immunology; D006681:HLA-D Antigens / Q000276:immunology*; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008459:Measles virus / Q000276:immunology*; D010835:Phytohemagglutinins; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D000956:Antigens, Viral; D000939:Epitopes; D006681:HLA-D Antigens; D007166:Immunosuppressive Agents; D010835:Phytohemagglutinins; D000001:Calcimycin; D013755:Tetradecanoylphorbol Acetate