Mutations located on both F1 and F2 subunits of the Newcastle disease virus fusion protein confer resistance to neutralization with monoclonal antibodies.
The fusion gene sequence of six Newcastle disease virus escape mutants revealed that residues important for the integrity of antigenic site 1 and antigenic site 2 were located, respectively, on the F2 subunit and within the cysteine-rich domain of the F1 subunit. We further report the antibody-binding capacity of these mutants.
Neyt
C
C
Laboratory of Biological Chemistry, Free University of Brussels, Belgium.
Geliebter
J
J
Slaoui
M
M
Morales
D
D
Meulemans
G
G
Burny
A
A
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000235:genetics* / Q000276:immunology; D000939:Epitopes / Q000235:genetics; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D009522:Newcastle disease virus / Q000235:genetics* / Q000276:immunology; D011485:Protein Binding; D014760:Viral Fusion Proteins / Q000235:genetics* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D014760:Viral Fusion Proteins