A suction blister method that gives epidermal biopsies from mice of different ages, suitable for histologic studies of Langerhans cells.
A modified suction blister device has been developed. It allows in vivo retrieval of 3 x 3 mm2 mouse epidermis biopsies. Separation takes place exactly at the epidermal-dermal junction. Young skin and iso-grafted skin require suction with a lower bar value than skin from young and old non-grafted animals. Langerhans cells in biopsies were identified both with ATPase staining and with monoclonal antibody. Suction blisters produce Langerhans cell counts similar to those obtained on EDTA-separated epidermis.
Langkjer
S T
ST
Danish Cancer Society, Department of Tumor Virology, Aarhus.
Villadsen
J H
JH
Bjerring
P
P
Ebbesen
P
P
D000375:Aging / Q000473:pathology; D000818:Animals; D001706:Biopsy / Q000295:instrumentation*; D002452:Cell Count / Q000379:methods; D004492:Edetic Acid; D004817:Epidermis / Q000473:pathology*; D007801:Langerhans Cells / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016038:Skin Transplantation; D013194:Staining and Labeling / Q000379:methods; D013396:Suction / Q000295:instrumentation*
D004492:Edetic Acid