Glycoprotein mannosylation in rat liver nuclei.
Nuclei and non-nuclear membranes were tested for their ability to transfer in vitro (14C)mannose from GDP-(14C)mannose to endogenous glycoprotein acceptors in the presence and in the absence of exogenous retinyl-phosphate. Electrophoretic analysis shows that retinylphosphate is responsible for the labeling of a few endogenous acceptors only in the non-nuclear membranes; in the nuclei the mannosylation reaction is not retinylphosphate dependent and the electrophoretic profile of the labeled protein acceptors is different from that of the non-nuclear membranes.
Fayet
Y
Y
Laboratoire de Biochimie des Membranes LBTM--CNRS, Villeurbane--France.
Galland
S
S
Degiuli
A
A
Got
R
R
Frot-Coutaz
J
J
D000818:Animals; D001345:Autoradiography; D002250:Carbon Radioisotopes; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D004224:Diterpenes; D006023:Glycoproteins / Q000096:biosynthesis*; D006155:Guanosine Diphosphate Mannose / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008358:Mannose / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D008854:Microscopy, Electron; D009687:Nuclear Proteins / Q000096:biosynthesis*; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D011103:Polyisoprenyl Phosphate Monosaccharides / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D002250:Carbon Radioisotopes; D004224:Diterpenes; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D009687:Nuclear Proteins; D009702:Nucleoside Diphosphate Sugars; D011103:Polyisoprenyl Phosphate Monosaccharides; D006155:Guanosine Diphosphate Mannose; C013101:mannosylretinylphosphate; D008358:Mannose