A monoclonal antibody recognizes a subset of the H-2Dd mouse major class I antigens.
Binding studies and competition experiments have shown that a monoclonal antibody (mAb) named 28-8-6 recognizes only 5 to 10% of the cell surface Dd molecules. The molecules detected by 28-8-6 mAb appear to be genuine H-2Dd antigens on the basis of their MW and isolectric points. In addition, the detectability of the subset of cell surface Dd molecules by 28-8-6 does not depend on their degree of glycosylation nor on the presence of mouse beta-2-microglobulin. Several interpretations are discussed. mAb 28-8-6 might detect a particular conformation or a particular chemical derivatization of otherwise normal H-2Dd molecules. Also, because the epitope recognized by 28-8-6 lies close to the peptide binding site, it is possible that mAb 28-8-6 recognizes a subset of Dd molecules bearing a certain category of self peptides.
Abastado
J P
JP
Unité de Biologie Moléculaire du Gène, U277 INSERM, UAC 115 CNRS, Institut Pasteur, Paris.
Darche
S
S
Jouin
H
H
Delarbre
C
C
Gachelin
G
G
Kourilsky
P
P
D000818:Animals; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology; D006183:H-2 Antigens / Q000145:classification* / Q000235:genetics; D007739:L Cells / Q000276:immunology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008968:Molecular Conformation; D013154:Spleen / Q000276:immunology; D014162:Transfection
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006183:H-2 Antigens