title: Human monoclonal antibodies to HIV-1: cross-reactions with gag and env products.
abstract: Human monoclonal antibodies were raised by in vitro immunization of normal human spleen cells with denatured HIV-1 and subsequent fusion to an Epstein-Barr virus (EBV) transformed cell line. Three monoclonals reacted with both gag-encoded p24 core protein and env-encoded gp41 transmembrane glycoprotein. The cross-reaction was confirmed by reactivity with p24- and gp41-derived recombinant peptides. The peptides share two amino acid sequences which may be the basis for the cross-reaction. Attempted epitope mapping using synthetic peptides was unsuccessful due to non-specific reactivity with the short linear peptides.
authors: Department of Nephrology, Royal Melbourne Hospital, Victoria, Australia.
authors: B J
authors: BJ
authors: Pollock
authors: A S
authors: AS
authors: McKenzie
authors: B E
authors: BE
authors: Kemp
authors: D A
authors: DA
authors: McPhee
authors: A J
authors: AJ
authors: D'Apice
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D015683:Gene Products, gag / Q000276:immunology*; D015483:HIV Antibodies / Q000276:immunology*; D015488:HIV Antigens / Q000276:immunology; D016655:HIV Core Protein p24; D015700:HIV Envelope Protein gp41 / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D014758:Viral Core Proteins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D015683:Gene Products, gag; D015483:HIV Antibodies; D015488:HIV Antigens; D016655:HIV Core Protein p24; D015700:HIV Envelope Protein gp41; D014758:Viral Core Proteins