title: Immunohistochemical study of tissue polypeptide antigen (TPA) and cancer antigen 125 (CA125) in the human and cynomolgus monkey placenta, umbilical cord and decidua.
abstract: Tissue polypeptide antigen (TPA) and cancer antigen 125 (CA125) were studied immunohistochemically by the avidin-biotin immunoperoxidase technique in human and cynomolgus monkey placentae, membranes, umbilical cords and decidua. In early human placentae, TPA was localized mainly in the cell membranes of villous syncytio- and cyto-trophoblast. The cytoplasm of those trophoblastic cells were weakly stained with TPA. The membrane of basal chorionic trophoblast cells was strongly stained with TPA and the cytoplasm stained weakly. In early cynomolgus placentae, similar immunostaining results were obtained. However, the positive stainings for TPA was more marked in the cytoplasm of villous syncytiotrophoblast and basal chorionic trophoblast, and less marked in the cell membrane of villous cytotrophoblast. In early human and cynomolgus placentae, CA125 was not demonstrated immunohistochemically in the villi and basal chorion. In human and cynomolgus term placentae, the villous syncytiotrophoblast and basal and reflected chorionic trophoblast showed similar immunostaining as the early placentae. In addition, TPA was found in the amniotic epithelium in both sorts of placentae. TPA was not detected immunohistochemically in the umbilical cord and decidual cells. While weakly positive stains for CA125 were observed in decidual cells, CA125 was localized mainly in the membrane and cytoplasm of amniotic epithelium in both human and cynomolgus term placentae. TPA and CA125 are thus oncoplacental antigens and the monkey could serve as a model for their investigation.
authors: Division of Obstetrics and Gynecology, Hospital of Kawasaki Steel Corporation Health Insurance Society, Chiba, Japan.
authors: K
authors: K
authors: Itahashi
authors: N
authors: N
authors: Inaba
authors: I
authors: I
authors: Fukazawa
authors: H
authors: H
authors: Takamizawa
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D015295:Antigens, Tumor-Associated, Carbohydrate; D003656:Decidua / Q000032:analysis*; D000939:Epitopes; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008252:Macaca fascicularis; D010455:Peptides / Q000032:analysis*; D010920:Placenta / Q000032:analysis*; D019396:Tissue Polypeptide Antigen; D014470:Umbilical Cord / Q000032:analysis*
chemical_list: D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D000939:Epitopes; D010455:Peptides; D019396:Tissue Polypeptide Antigen