Effect of peptide AS-48 on Enterococcus faecalis subsp. liquefaciens S-47.
The enterococcal peptide AS-48 exerts a concentration-dependent bactericidal effect on Enterococcus faecalis subsp. liquefaciens S-47; cell rescue by cardiolipin and trypsin can be effected only in the first few minutes after antibiotic addition. Gramicidin-exposed cells are protected from killing by AS-48. Long-term and pulse incorporation of radiolabeled substrates into trichloroacetic acid-precipitable material, O2 consumption, and the ability to maintain intracellular potassium levels are impaired shortly after addition of AS-48.
Galvez
A
A
Departamento de Microbiologia, Facultad de Ciencias, Universidad de Granada, Spain.
Valdivia
E
E
Martinez
M
M
Maqueda
M
M
D000900:Anti-Bacterial Agents / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000096:biosynthesis; D002308:Cardiolipins / Q000494:pharmacology; D004269:DNA, Bacterial / Q000096:biosynthesis; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000378:metabolism; D006096:Gramicidin / Q000494:pharmacology; D007930:Leucine / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D010100:Oxygen / Q000378:metabolism; D010455:Peptides / Q000037:antagonists & inhibitors / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D014357:Trypsin / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002308:Cardiolipins; D004269:DNA, Bacterial; D010455:Peptides; C051034:BacA protein, Enterococcus faecalis; D006096:Gramicidin; D012964:Sodium; D014357:Trypsin; D007930:Leucine; D011188:Potassium; D010100:Oxygen