Radioimmunoassay of human pancreatic amylase with monoclonal antibody.
A monoclonal antibody (E-21) was obtained that specifically binds to human pancreatic amylase and shows negligible cross-reaction with human salivary amylase. Using this antibody a radioimmunoassay was developed for pancreatic amylase in human serum. The assay was shown to be sensitive (detectable up to 7 mg/L), reproducible, and specific for pancreatic amylase. In normal subjects, the mean concentration of serum pancreatic amylase determined by this method was 36.3 mg/L with a 95% confidence range of 16.5 to 79.2 mg/L. A good correlation was observed between the concentrations of immunoreactive pancreatic amylase (IR-PA) and enzymatic activities in 20 serum samples (r = 0.97). The concentration of serum IR-PA was below the detectable limit in pancreatectomised patients, and was greatly increased in patients with acute pancreatitis; the latter was accompanied by parallel changes in total enzymic activity. In patients with mumps, the serum IR-PA level was within the normal range whereas the total enzymic activity was elevated.
Fujita
C
C
Department of Laboratory Medicine, School of Medicine, University of Tokushima, Japan.
Kasai
C
C
Kosuge
H
H
Ogata
K
K
Oshima
I
I
Shima
K
K
D000681:Amylases / Q000097:blood* / Q000302:isolation & purification; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D005260:Female; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D007457:Iodine Radioisotopes; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D010179:Pancreas / Q000201:enzymology*; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes; D000681:Amylases