The complete sequence of the human intermediate filament chain keratin 10. Subdomainal divisions and model for folding of end domain sequences.
We present the complete amino acid sequence of the human keratin 10 (type I) intermediate filament chain expressed in terminally differentiated epidermal cells. Comparisons of this sequence with its mouse and bovine counterparts allow us to describe structural features of the functional end domains. First, sections of their respective end domains are highly conserved and permit a redefinition of earlier models for their subdomainal organization. The amino-terminal end domain consists of El, the first 57-58 residues that are basic, glycine-rich, and have been highly conserved among the three species; V1, a region of well-defined quasi repeats of the motif aliphatic-serine/glycinen; and H1, a newly recognized short acidic sequence that has been conserved among the type I keratin family. The carboxyl-terminal end consists of V2 and E2 whose properties but not sequence resemble V1 and E1, respectively. Second, since the E1, H1, and E2 sequences have been highly conserved between the three species, we suggest they are critical elements in defining intermediate filament function. Third, we note that the E and V sequences of the keratin 10 (and other keratin) chains share many properties in common with protein chain turns found in globular proteins. We therefore propose a model in which these sequences form omega loop-like structures (Leszczynski, J. N. & Rose, G. D. (1986) Science 234, 849-855) on the surface of keratin intermediate filaments. This represents the first specific proposal for the end domain structure of any intermediate filament chain.
Zhou
X M
XM
Dermatology Branch, National Cancer Institute, Bethesda, Maryland 20892.
Idler
W W
WW
Steven
A C
AC
Roop
D R
DR
Steinert
P M
PM
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002417:Cattle; D004247:DNA / Q000032:analysis; D006801:Humans; D007633:Keratins / Q000032:analysis* / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism
D000596:Amino Acids; D012333:RNA, Messenger; D007633:Keratins; D004247:DNA