Chemical and Mössbauer spectroscopic evidence that iron-containing concanavalin A is a ferritin.
We report here the preparation of iron-containing concanavalin A. It has a protein-to-iron ratio of 2.0, and the iron compound it contains is particulate with an average diameter of 85 A. Iron-containing concanavalin A interacts reversibly with dextran and with methyl alpha-D-glucoside. The molecular basis of these findings is discussed and a possible mechanism suggested where one of the molecular forms of concanavalin A has the structure of an apoferritin into which iron is deposited in the form of ferrihydrite.
Yariv
J
J
Department of Biophysics, Weizmann Institute of Science, Rehovot, Israel.
Kalb
A J
AJ
Helliwell
J R
JR
Papiz
M Z
MZ
Bauminger
E R
ER
Nowik
I
I
D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002621:Chemistry; D003208:Concanavalin A / Q000032:analysis; D003911:Dextrans; D005293:Ferritins; D005296:Ferrous Compounds; D007501:Iron / Q000032:analysis; D046911:Macromolecular Substances; D008757:Methylglucosides; D008970:Molecular Weight; D011487:Protein Conformation; D000644:Quaternary Ammonium Compounds; D013051:Spectrometry, Gamma
D003911:Dextrans; D005296:Ferrous Compounds; D046911:Macromolecular Substances; D008757:Methylglucosides; D000644:Quaternary Ammonium Compounds; D003208:Concanavalin A; C027020:methylglucoside; D005293:Ferritins; C038178:ammonium ferrous sulfate; D007501:Iron