title: Electrophoretic characterization and immunoblot analysis of the proteins from the myelin-like light membrane fraction of shrimp ventral nerve (Penaeus duorarum).
abstract: 1. The proteins of the light membrane fraction (LMF) from the ventral nerve of the pink shrimp (Penaeus duorarum) were separated by SDS gel electrophoresis and analysed by staining and immunoblotting. 2. Shrimp LMF carried four major proteins with apparent molecular weights of Mr = 21,500, 40,000, 78,000, 85,000 and four minor components (Mr = 36,000, 41,500, 43,000, 50,000). 3. None of these proteins bound Concanavalin A. 4. The four major proteins showed no reaction with antisera against six vertebrate myelin proteins. Only the minor Mr = 50,000 component was weakly recognized by the antibodies against mammalian myelin P0 protein.
authors: Max-Planck-Institut für experimentalle Medizin, Forschungsstelle Neurochemie, Göttingen, FRG.
authors: T V
authors: TV
authors: Waehneldt
authors: J
authors: J
authors: Malotka
authors: S
authors: S
authors: Kitamura
authors: Y
authors: Y
authors: Kishimoto
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D007120:Immunochemistry; D008565:Membrane Proteins / Q000276:immunology / Q000302:isolation & purification*; D008970:Molecular Weight; D004676:Myelin Basic Protein / Q000276:immunology / Q000302:isolation & purification; D009419:Nerve Tissue Proteins / Q000276:immunology / Q000302:isolation & purification*; D033561:Penaeidae / Q000032:analysis*
chemical_list: D008565:Membrane Proteins; D004676:Myelin Basic Protein; D009419:Nerve Tissue Proteins