DNA binding, cytotoxicity and inhibitory effect on RNA synthesis of two new 1-nitro-9-aminoacridine dimers.
Two 1-nitro-9-aminoacridine dimers were prepared: one bearing a spermine flexible linking chain, compound 4, the other a rigid dipiperidine-type linker, compound 7. Both dimers elicited a higher affinity constant for DNA than the parent monomeric drug nitracrine 2. This affinity was several orders lower than what was found for other dimeric compounds having the same linkers and no nitro group on the acridine ring (3, 5, 6 and 8). Bisintercalation was evidenced for compound 4 by viscosimetric measurements. In the absence of dithiothreitol, an inhibitory effect of RNA synthesis in vitro was observed for all the tested compounds except 2 and 7. In the presence of dithiothreitol, 4 and 7 formed irreversible complexes with DNA of decreased template properties. The level of the dimers binding was lower than that of the parent compound 2. Cross-links were detected by means of hydroxylapatite chromatography in a complex of the dimer bearing a flexible linking chain, compound 4 with DNA, while the compound 7-DNA complex eluted in the single-stranded DNA region. The extent of cytotoxicity of the two 1-nitro-9-aminoacridine dimers against L1210 cultured cells was different.
Markovits
J
J
Laboratoire de Pharmacologie Moléculaire, UA 147 (CNRS), U 140 (INSERM) Institut Gustave Roussy, Villejuif, France.
Wilmańska
D
D
Lescot
E
E
Studzian
K
K
Szmigiero
L
L
Gniazdowski
M
M
D000609:Aminoacridines / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis; D004247:DNA / Q000378:metabolism*; D004229:Dithiothreitol / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D007364:Intercalating Agents / Q000138:chemical synthesis; D007939:Leukemia L1210 / Q000188:drug therapy; D051379:Mice; D012313:RNA / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
D000609:Aminoacridines; D000970:Antineoplastic Agents; D007364:Intercalating Agents; D012313:RNA; D004247:DNA; D004229:Dithiothreitol