Presence of a charybdotoxin sensitive Ca2+-activated K+ channel in rat glioma C6 cells.
A study was made of the 86Rb+ influx and efflux through Ca2+-activated K+ channels of intact rat glioma C6 cells after addition of a Ca2+ ionophore to the incubation medium. Half-maximal activation of the channels was obtained at a cytoplasmic Ca2+ concentration of approximately 400 nM. The 86Rb+ ion flux through the Ca2+-activated K+ channels was insensitive to apamin, but was inhibited by low concentrations of charybdotoxin (IC50 = 1.6 nM). This is the first evidence for the presence of charybdotoxin-sensitive Ca2+-activated K+ channels in glial cells.
Tas
P W
PW
Institute for Virology and Immunology, University of Würzburg, F.R.G.
Kress
H G
HG
Koschel
K
K
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology*; D018999:Charybdotoxin; D004987:Ethers / Q000494:pharmacology; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D015759:Ionomycin; D015221:Potassium Channels / Q000378:metabolism*; D012413:Rubidium / Q000037:antagonists & inhibitors / Q000378:metabolism; D012414:Rubidium Radioisotopes; D012604:Scorpion Venoms / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D004987:Ethers; D015221:Potassium Channels; D012414:Rubidium Radioisotopes; D012604:Scorpion Venoms; D018999:Charybdotoxin; D000001:Calcimycin; D015759:Ionomycin; D012413:Rubidium; D002118:Calcium