title: Canine and bovine ras family expression detected and discriminated by use of polymerase chain reaction.
abstract: Cellular proto-oncogenes are highly conserved genes thought to be critical in cell growth and differentiation. In this study, we used human sequence designed oligonucleotide primers to detect and discriminate c-Ha-ras-1, c-Ki-ras-2 and c-N-ras genes of dogs and cows by polymerase chain reaction (PCR) amplification of genomic DNA (DNA/PCR). Further, we have applied PCR for analysis of expressed mRNA transcribed from the RAS genes (RNA/PCR).
authors: Department of Veterinary Surgery, University of California School of Veterinary Medicine, Davis 95616.
authors: B R
authors: BR
authors: Madewell
authors: P H
authors: PH
authors: Gumerlock
authors: K A
authors: KA
authors: Saunders
authors: F J
authors: FJ
authors: Meyers
authors: S A
authors: SA
authors: Kraegel
authors: E S
authors: ES
authors: Kawasaki
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002478:Cells, Cultured; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004285:Dogs; D015870:Gene Expression; D011905:Genes, ras; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D014407:Tumor Cells, Cultured / Q000378:metabolism
chemical_list: D015345:Oligonucleotide Probes; D012313:RNA; D004247:DNA