Inhibition of reverse transcription in rat liver intracisternal A-particles by thymidine derivatives.
The thymidine derivatives araAzT, dTTP(3'N3), TTP(3'NH2), and araTTP(3'N3), were studied as inhibitors of the reverse transcription taking place within endogenous retroviral A-type particles, where retroviral RNAs served as templates and primers, dTTP(3'N3) was shown to be the most efficient inhibitor of retroviral particle reverse transcription. Termination of DNA chain elongation is the basic mechanism of the inhibitory action of dTTP(3'N3). The compound has a very low inhibitory effect on mammalian DNA-dependent DNA polymerases alpha, beta and gamma.
Pyrinova
G B
GB
Institute of Cytology and Genetics, Siberian Department of the USSR Academy of Sciences, Novosibirsk.
Kuzminova
E A
EA
Salganik
R I
RI
Krayevsky
A A
AA
Kukhanova
M M
MM
D000818:Animals; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects; D054306:Dideoxynucleotides; D007426:Genes, Intracisternal A-Particle; D008862:Microsomes, Liver / Q000382:microbiology*; D011519:Proto-Oncogenes; D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase / Q000235:genetics; D051381:Rats; D011919:Rats, Inbred Strains; D012190:Retroviridae / Q000235:genetics; D018894:Reverse Transcriptase Inhibitors; D013698:Templates, Genetic; D013936:Thymidine / Q000031:analogs & derivatives* / Q000494:pharmacology; D013942:Thymine Nucleotides / Q000494:pharmacology; D015215:Zidovudine / Q000031:analogs & derivatives / Q000494:pharmacology
D054306:Dideoxynucleotides; D012367:RNA, Viral; D018894:Reverse Transcriptase Inhibitors; D013942:Thymine Nucleotides; D015215:Zidovudine; C052873:zidovudine triphosphate; D004247:DNA; D012194:RNA-Directed DNA Polymerase; D013936:Thymidine