Autoreactive T-cell response to resting or activated B cells.
Cloned autoreactive T-cell lines and hybridomas have been selected in many laboratories. A number of observations have suggested that activation of Ia-positive stimulators may be required for optimal induction of an autoreactive response. We have examined the ability of small resting B cells fractionated by centrifugal elutriation to stimulate the proliferative response of seven cloned autoreactive T-cell lines. Of these, six were efficiently stimulated by resting B cells. One I-Ed-specific Th2-type T-cell clone failed to be stimulated by resting B cells. This clone did, however, respond to this same cell fraction following lipopolysaccharide (LPS) activation. An independent I-E-specific Th2 clone was stimulated by resting B cells. It appears, therefore, that a requirement for activated stimulators is not a general property of either autoreactive T cells or the Th2 helper T-cell subset.
Moynihan
J
J
Cancer Center, University of Rochester Medical Center, New York 14642.
Burstyn
D
D
Zauderer
M
M
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002450:Cell Communication; D002460:Cell Line; D002999:Clone Cells; D000939:Epitopes / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
D000939:Epitopes