Production and characterization of monoclonal antibodies specific for Mycobacterium bovis.
A series of monoclonal antibodies (MAbs), specific for Mycobacterium bovis and BCG strains, were tested extensively for cross-reactivity to a wide range of mycobacterial species using ELISA, Western blotting and dot-blot analysis. The MAbs bound specifically to M. bovis and BCG and showed limited cross-reactivity with some strains of M. tuberculosis. All these MAbs recognized a 22 kDa protein previously termed MPB70, and by competitive ELISA analysis appeared to detect at least three M. bovis-specific determinants on the MPB70 molecule.
Wood
P R
PR
CSIRO Division of Animal Health, Animal Health Research Laboratory, Parkville, Vic., Australia.
Ripper
J
J
Radford
A J
AJ
Bundesen
P G
PG
Rylatt
D B
DB
Cottis
L E
LE
John
M
M
Plackett
P
P
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D000918:Antibody Specificity; D015153:Blotting, Western; D002417:Cattle; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009161:Mycobacterium / Q000276:immunology; D009163:Mycobacterium bovis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes