The effect of dimethyl PGE2 on canine pancreatic autograft exocrine secretion.
This study was designed to evaluate the effect of 16,16-dimethyl prostaglandin E2 (dimethyl PGE2) on exocrine secretion in autografted animals with pancreaticocystostomies. The rates of secretion of urinary (autograft) amylase (units/min) and bicarbonate (mmole/min), over a 5-hr interval, were determined in the basal state (Group A, N = 5), after a bolus injection of 1 microgram/kg of dimethyl PGE2 (Group B, N = 5), during an OP-CCK infusion at 125 ng/kg/hr (Group C, N = 5), and during an OP-CCK infusion plus a bolus injection of 1 microgram/kg (Group D, N = 5) or 18 micrograms/kg (Group E, N = 5) of dimethyl PGE2 at the end of the second hour. Basal secretion of amylase and bicarbonate were decreased 1 hr after 1 microgram/kg of dimethyl PGE2, with the bicarbonate inhibition being statistically significant (Group A = 0.073 +/- 0.04 vs Group B = 0.001 +/- 0.00; P less than 0.05). When compared to Group C (128.3 +/- 28.0), an immediate and significant inhibition of OP-CCK-stimulated amylase release was demonstrated in both Group D (36.3 +/- 11.1; P less than 0.02) and Group E (57.3 +/- 13.4; P less than 0.05). One and two hours post-dimethyl PGE2, amylase releases were 37.7 +/- 8.6 and 64.7 +/- 6.8 in Group D and 0.92 +/- 0.3 and 8.28 +/- 2.6 in Group E, compared to 140.3 +/- 23.3 and 104.9 +/- 31.8 in Group C, indicating a dose-related, prolonged inhibition of autograft amylase secretion.(ABSTRACT TRUNCATED AT 250 WORDS)
Garvin
P J
PJ
Transplant Unit, John Cochran Veterans Administration Medical Center, Saint Louis, Missouri.
Niehoff
M
M
Burton
F
F
D015064:16,16-Dimethylprostaglandin E2 / Q000008:administration & dosage / Q000494:pharmacology*; D000681:Amylases / Q000378:metabolism* / Q000652:urine; D000818:Animals; D001639:Bicarbonates / Q000378:metabolism* / Q000652:urine; D004285:Dogs; D005260:Female; D007262:Infusions, Intravenous; D010179:Pancreas / Q000378:metabolism; D016035:Pancreas Transplantation; D011459:Prostaglandins E, Synthetic / Q000494:pharmacology*; D011897:Random Allocation; D013997:Time Factors; D014182:Transplantation, Autologous
D001639:Bicarbonates; D011459:Prostaglandins E, Synthetic; D000681:Amylases; D015064:16,16-Dimethylprostaglandin E2