Adenosine-5'-triphosphate-sensitive ion channels in neonatal rat cultured central neurones.
ATP-sensitive channels were observed in isolated inside-out membrane patches from rat cultured central neurones. Two types of ATP-sensitive K+ channels were present in cortical neurones, one which had its open-state probability increased, the other its open-state probability decreased by application of ATP to the cytoplasmic membrane surface. Another, ATP-sensitive channel differing in ion conductance from all previously reported ATP-sensitive channels was also seen in patches from cortical neurones. This channel was nonselective with respect to Na+, K+ and Cl- ions and ATP produced a "flickery" type of block. The non-hydrolysable analogue, AMPPNP, did not mimic ATP and prevented ATP action. Preliminary experiments indicate that similar, but not, identical ATP-sensitive channels exist in cerebellar neurones.
Ashford
M L
ML
Department of Pharmacology, University of Cambridge, Great Britain.
Sturgess
N C
NC
Trout
N J
NJ
Gardner
N J
NJ
Hales
C N
CN
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D007473:Ion Channels / Q000187:drug effects*; D009474:Neurons / Q000378:metabolism*; D015221:Potassium Channels / Q000187:drug effects; D051381:Rats
D007473:Ion Channels; D015221:Potassium Channels; D000255:Adenosine Triphosphate