Stimulation of plasmin catalyzed conversion of single-chain to two-chain urokinase-type plasminogen activator by sulfated polysaccharides.
Influence of heparin, chondroitin sulfate C and dextran sulfate (MW 3,500 and 7,000) on plasmin catalyzed conversion of single-chain (scu-PA) to two-chain (u-PA) urokinase-type plasminogen activator and generation of plasmin in mixtures of scu-PA and Glu-plasminogen (Glu-plg) was investigated. Conversion of scu-PA to u-PA catalyzed by plasmin was enhanced by chondroitin sulfate C and heparin, maximally by 10-fold and 3-fold, respectively. Sulfated polysaccharides potentiated generation of plasmin in mixtures of Glu-plg and scu-PA, as well as enhanced plasminogenolytic activity of u-PA. The possible role of these interactions is discussed.
Rydzewski
A
A
Department of Physiology, Hamamatsu University, School of Medicine, Shizuoka-ken, Japan.
Takada
Y
Y
Takada
A
A
D002384:Catalysis; D002809:Chondroitin Sulfates / Q000494:pharmacology; D003911:Dextrans / Q000494:pharmacology; D004357:Drug Synergism; D005341:Fibrinolysin / Q000096:biosynthesis / Q000502:physiology*; D005343:Fibrinolytic Agents / Q000378:metabolism* / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D008970:Molecular Weight; D010958:Plasminogen / Q000494:pharmacology; D010960:Plasminogen Activators / Q000378:metabolism* / Q000494:pharmacology; D011134:Polysaccharides / Q000494:pharmacology*; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism* / Q000494:pharmacology
D003911:Dextrans; D005343:Fibrinolytic Agents; D011134:Polysaccharides; D010958:Plasminogen; D006493:Heparin; D002809:Chondroitin Sulfates; D010960:Plasminogen Activators; D005341:Fibrinolysin; D014568:Urokinase-Type Plasminogen Activator