Analysis of three-dimensional cell imaging obtained with optical microscopy techniques based on defocusing.
The properties of an optical microscope are analyzed and analytically evaluated with a simple and effective model in order to understand the true meaning, limitations, and real capabilities of a defocusing technique. Major emphasis is given to the applications related to microscopic objects of biological interest using fluorescence and absorption light microscopy. A procedure for three-dimensional viewing is analyzed and discussed.
Bianco
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B
Department of Biophysical and Electronic Engineering, Genova, Italy.
Diaspro
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D003584:Cytological Techniques; D007091:Image Processing, Computer-Assisted; D008433:Mathematics; D008853:Microscopy / Q000379:methods*; D008962:Models, Theoretical; D055095:Optics and Photonics