Analysis of compound 48/80-induced skin histamine release and leukotriene production in chronic urticaria.
Profiles of histamine release by nonlesional skin mast cells from patients with chronic urticaria (CU) and normal control (NC) subjects were compared on stimulation with a wide range of concentrations of compound 48/80 (0.15 to 4.8 mg/ml). One previous observation demonstrating spontaneous and 48/80-induced release of histamine greater in patients with CU than in NC subjects was confirmed with a concentration of 48/80 believed to produce mast cell activation in most subjects (2.4 mg/ml). In fact, higher concentrations of histamine were released by patients with CU than by NC subjects on stimulation with 48/80 at concentration greater than 0.6 mg/ml. With 0.6 mg/ml of 48/80, the profiles of histamine release were comparable in the two groups, and for concentration less than 0.6 mg/ml, the profiles of release were completely different, being higher in NC subjects than in the group with CU. The peak histamine response was higher in the group of NC subjects (6500 pg/ml) than in the group with CU (5100 pg/ml), and the concentration of 48/80 needed to trigger maximum release was lower in the NC subjects than in the subjects with CU (0.15 versus 0.6 mg/ml). The production of leukotriene B4 and leukotriene C4 was also compared in these two groups after stimulation with 2.4 mg/ml of compound 48/80. No significant amount of leukotriene B4 was found in the collection chambers, but small amounts of leukotriene C4 were measured into skin chamber fluids during a period of 4 hours. No significant difference could then be observed between the two groups.
Brunet
C
C
Unité de Recherche Inflammation et Immunologie-Rhumatologie, Centre Hospitlier de l'Université Laval, Ste-Foy, Québec, Canada.
Bédard
P M
PM
Hébert
J
J
D000328:Adult; D002908:Chronic Disease; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D015289:Leukotrienes / Q000096:biosynthesis*; D008407:Mast Cells / Q000503:physiopathology; D013189:SRS-A / Q000096:biosynthesis*; D012867:Skin / Q000503:physiopathology; D014581:Urticaria / Q000503:physiopathology*; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology*
D015289:Leukotrienes; D013189:SRS-A; D003189:p-Methoxy-N-methylphenethylamine