The PROS-35 gene encodes the 35 kd protein subunit of Drosophila melanogaster proteasome.
The proteasome is a multicatalytic proteinase complex composed of nonidentical protein subunits. We have isolated a cDNA clone encoding the 35 kd proteasome subunit of Drosophila melanogaster and propose the designation PROS-35 for the corresponding gene. The deduced amino acid sequence reveals a region of striking homology to a tyrosine phosphorylation site of viral and cellular proteins suggesting a potential regulatory function for the 35 kd subunit within the proteinase complex. Immunocytochemical experiments reveal a tissue-dependent differential distribution of the proteasome between the nucleus and cytoplasm. In addition developmental analysis shows that the proteasome is highly expressed in the CNS of stage-16 embryos and in cardia, ventriculus and ovaries of adult flies. These data suggest a tissue- and development-dependent distribution of the proteasome in D. melanogaster.
Haass
C
C
ZMBH, Heidelberg, FRG.
Pesold-Hurt
B
B
Multhaup
G
G
Beyreuther
K
K
Kloetzel
P M
PM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002846:Chromatography, Affinity; D003546:Cysteine Endopeptidases / Q000032:analysis / Q000235:genetics*; D004247:DNA / Q000235:genetics; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics*; D010450:Endopeptidases / Q000032:analysis / Q000235:genetics*; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000032:analysis / Q000235:genetics*; D010766:Phosphorylation; D011061:Poly A / Q000235:genetics; D046988:Proteasome Endopeptidase Complex; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014443:Tyrosine / Q000378:metabolism
D009097:Multienzyme Complexes; D012333:RNA, Messenger; D011061:Poly A; D014443:Tyrosine; D012313:RNA; D004247:DNA; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex