title: Intracellular mitochondrial membrane potential as an indicator of hepatocyte energy metabolism: further evidence for thermodynamic control of metabolism.
abstract: The lipophilic triphenylmethylphosphonium cation (TPMP+) has been employed to measure delta psi m, the electrical potential across the inner membrane of the mitochondria of intact hepatocytes. The present studies have examined the validity of this technique in hepatocytes exposed to graded concentrations of inhibitors of mitochondrial energy transduction. Under these conditions, TPMP+ uptake allows a reliable measure of delta psi m in intracellular mitochondria, provided that the ratio [TPMP+]i/[TPMP+]e is greater than 50:1 and that at the end of the incubation more than 80% of the hepatocytes exclude Trypan blue. Hepatocytes, staining with Trypan blue, incubated in the presence of Ca2+, do not concentrate TPMP+. The relationships between delta psi m and two other indicators of cellular energy state, delta GPc and Eh, or between delta psi m and J0, were examined in hepatocytes from fasted rats by titration with graded concentrations of inhibitors of mitochondrial energy transduction. Linear relationships were generally observed between delta psi m and delta GPc, Eh or J0 over the delta psi m range of 120-160 mV, except in the presence of carboxyatractyloside or oligomycin, where delta psi m remained constant. Both the magnitude and the direction of the slope of the observed relationships depended upon the nature of the inhibitor. Hepatocytes from fasted rats synthesized glucose from lactate or fructose, and urea from ammonia, at rates which were generally linear functions of the magnitude of delta psi m, except in the presence of oligomycin or carboxyatractyloside. Linear relationships were also observed between delta psi m and the rate of formation of lactate in cells incubated with fructose and in hepatocytes from fed rats. The linear property of these force-flow relationships is taken as evidence for the operation of thermodynamic regulatory mechanisms within hepatocytes.
authors: Department of Medical Biochemistry, School of Medicine, Flinders University of South Australia, Bedford Park.
authors: M N
authors: MN
authors: Berry
authors: R B
authors: RB
authors: Gregory
authors: A R
authors: AR
authors: Grivell
authors: D C
authors: DC
authors: Henly
authors: C D
authors: CD
authors: Nobes
authors: J W
authors: JW
authors: Phillips
authors: P G
authors: PG
authors: Wallace
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D001278:Atractyloside / Q000031:analogs & derivatives / Q000494:pharmacology; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D004734:Energy Metabolism / Q000187:drug effects; D005943:Gluconeogenesis / Q000187:drug effects; D007202:Indicators and Reagents; D007425:Intracellular Membranes / Q000502:physiology; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008564:Membrane Potentials; D008930:Mitochondria, Liver / Q000187:drug effects / Q000502:physiology*; D009840:Oligomycins / Q000494:pharmacology; D009861:Onium Compounds; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling; D013816:Thermodynamics; D014320:Triphenylmethyl Compounds; D014343:Trypan Blue; D014508:Urea / Q000096:biosynthesis; D014634:Valinomycin / Q000494:pharmacology
chemical_list: D000227:Adenine Nucleotides; D007202:Indicators and Reagents; D009840:Oligomycins; D009861:Onium Compounds; D014320:Triphenylmethyl Compounds; C011561:triphenylmethylphosphonium; D001278:Atractyloside; D014634:Valinomycin; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D014508:Urea; D014343:Trypan Blue; C003853:carboxyatractyloside