title: Blockage of alpha beta T-cell development by TCR gamma delta transgenes.
abstract: T lymphocytes recognize antigens by means of T-cell receptors (TCR) composed of alpha beta or gamma delta heterodimers. The mechanism governing the development of alpha beta- and gamma delta-bearing T cells from a common precursor T cell is so far unknown. It has been proposed that T-cell precursors rearrange their gamma- and delta-chain genes first, and alpha beta T cells are generated only from those cells that fail to rearrange productively both gamma- and delta-chain genes. Our recent study on gamma delta-transgenic mice contradicted this hypothesis, however, and indicated that repression of gamma-chain gene expression mediated by a transcriptional silencer element has a critical role in the generation of alpha beta T cells. Here we report that the generation of alpha beta T cells is severely blocked in transgenic mice carrying gamma- and delta-chain transgenes without the associated silencer, thereby strengthening the validity of the silencer model of T-cell development.
authors: Howard Hughes Medical Institute, Cambridge, Massachusetts 02139.
authors: M
authors: M
authors: Bonneville
authors: I
authors: I
authors: Ishida
authors: P
authors: P
authors: Mombaerts
authors: M
authors: M
authors: Katsuki
authors: S
authors: S
authors: Verbeek
authors: A
authors: A
authors: Berns
authors: S
authors: S
authors: Tonegawa
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D005260:Female; D015329:Gene Rearrangement, T-Lymphocyte; D046911:Macromolecular Substances; D051379:Mice; D008822:Mice, Transgenic / Q000276:immunology; D012313:RNA / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000235:genetics*; D015183:Restriction Mapping; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D046911:Macromolecular Substances; D011948:Receptors, Antigen, T-Cell; D012313:RNA; D004247:DNA