Glycosylation site binding protein, a component of oligosaccharyl transferase, is highly similar to three other 57 kd luminal proteins of the ER.
A 57 kd component of oligosaccharyl transferase, termed glycosylation site binding protein, specifically recognizes a photoaffinity probe containing the N-glycosylation site sequence Asn-Lys-Thr. It is present in the lumen of the ER (endoplasmic reticulum) and its release from this compartment results in a loss of N-glycosylation. Antibodies against this protein were used to identify cDNA clones from a lambda gt11 expression library. Analysis of its cDNA sequence reveals high sequence similarity to three other 57 kd luminal endoplasmic reticulum proteins: protein disulfide isomerase, the beta-subunit of prolyl hydroxylase, and thyroid hormone binding protein. This finding suggests that the capacity to recognize multiple polypeptide domains may reside in a single luminal protein that participates in co- and/or posttranslational modifications of newly synthesized proteins.
Geetha-Habib
M
M
Department of Biochemistry and Molecular Biology, University of Texas MD Anderson Cancer Center, Houston 77030.
Noiva
R
R
Kaplan
H A
HA
Lennarz
W J
WJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000502:physiology; D002645:Chickens; D004247:DNA; D004721:Endoplasmic Reticulum / Q000032:analysis*; D005260:Female; D006031:Glycosylation; D006602:Hexosyltransferases; D006801:Humans; D007535:Isomerases; D008565:Membrane Proteins / Q000502:physiology; D008954:Models, Biological; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D011393:Procollagen-Proline Dioxygenase; D019704:Protein Disulfide-Isomerases; D012313:RNA; D051381:Rats; D013963:Thyroid Hormones; D014166:Transferases / Q000032:analysis*; D000097810:Thyroid Hormone-Binding Proteins
D002352:Carrier Proteins; D004247:DNA; D006602:Hexosyltransferases; D007535:Isomerases; D008565:Membrane Proteins; D011393:Procollagen-Proline Dioxygenase; D019704:Protein Disulfide-Isomerases; D012313:RNA; D013963:Thyroid Hormones; D014166:Transferases; D000097810:Thyroid Hormone-Binding Proteins; C025620:dolichyl-diphosphooligosaccharide - protein glycotransferase