title: [Blocking action of Nephila clavata spider toxin on ionic currents activated by glutamate and its agonists in isolated hippocampal neurons].
abstract: The blocking action of the Nephila clavata spider neurotoxin was studied using the concentration clamp method in isolated neurons of the rat hippocampus. Crude venom JSTX blocked L-glutamate-, quisqualate- and kainate-activated ionic currents mediated by activation of the non-N-methyl-D-aspartate (non-NMDA) membrane receptors. Ionic currents elicited by all agonists were depressed by crude JSTX venom to 34-35% of its initial amplitude with no recovery during prolonged washing. An active fraction of JSTX venom blocked ionic currents almost completely, but its action was partially reversible. The concentration dependences of blocking kinetics allowed determining the rate constants of JSTX interaction with glutamate receptors. It is supposed that JSTX blocks the non-NMDA ionic channels in some of their open states and may be one of useful tools in further biochemical and electrophysiological characterization of the glutamate-mediated synaptic transmission.
authors: N I
authors: NI
authors: Kiskin
authors: E M
authors: EM
authors: Kliuchko
authors: O A
authors: OA
authors: Kryshtal'
authors: A Ia
authors: AIa
authors: Tsyndrenko
authors: N
authors: N
authors: Akaike
mesh_terms: D000818:Animals; D001180:Arthropod Venoms / Q000633:toxicity*; D005971:Glutamates / Q000494:pharmacology*; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects*; D007473:Ion Channels / Q000187:drug effects*; D007608:Kainic Acid / Q000494:pharmacology*; D007700:Kinetics; D009474:Neurons / Q000187:drug effects*; D010069:Oxadiazoles / Q000494:pharmacology*; D016318:Quisqualic Acid; D051381:Rats; D013111:Spider Venoms / Q000633:toxicity*
chemical_list: D001180:Arthropod Venoms; D005971:Glutamates; D007473:Ion Channels; C044936:JSTX spider toxin; D010069:Oxadiazoles; D013111:Spider Venoms; D016318:Quisqualic Acid; D007608:Kainic Acid
vernacular_title: Blokiruiushchee deĭstvie iada pauka Nephila clavata na ionnye toki, aktiviruemye glutamatom i ego agonistami v izolirovannykh neĭronakh gippokampa.