Monoclonal antibodies against different epitopes on colonization factor antigen I of enterotoxin-producing Escherichia coli.
Hybridoma-secreting monoclonal antibodies (MAbs) against colonization factor antigen I (CFA/I) were produced by the fusion of spleen cells from immunized BALB/c mice with F/O myeloma cells. The 25 MAbs with the highest antibody titer against CFA/I, as determined by an enzyme-linked immunosorbent assay, were studied for specificity and the capacity to agglutinate CFA/I-carrying bacteria. Most of the MAbs agglutinated a majority of 16 CFA/I-positive strains tested but not any of a number of CFA-deficient mutants or strains carrying other colonization factors (CFA/II and CFA/IV). One MAb that agglutinated all the CFA/I-positive strains and one MAb that did not agglutinate any of these strains were compared for their reactivities with different preparations of CFA/I. Whereas both MAbs bound to or could be inhibited by isolated CFA/I fimbriae as well as the subunit protein, only the agglutinating MAb bound to CFA/I, as expressed on whole bacteria. The nonagglutinating MAb, on the other hand, bound considerably better than the agglutinating MAb to a peptide corresponding to the 46 N-terminal amino acid residues of the CFA/I subunit protein. These results suggest that the two MAbs are directed against different epitopes on CFA/I.
Lopez-Vidal
Y
Y
Department of Medical Microbiology, University of Göteborg, Sweden.
Klemm
P
P
Svennerholm
A M
AM
D000371:Agglutination; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D004926:Escherichia coli / Q000276:immunology*; D005260:Female; D036781:Fimbriae Proteins; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes; C022924:colonization factor antigens; D036781:Fimbriae Proteins