Microinjection of antisense c-mos oligonucleotides prevents meiosis II in the maturing mouse egg.
Injection of antisense oligonucleotides was used to investigate the function of c-mos in murine oocytes. Oocytes injected with antisense c-mos oligonucleotides completed the first meiotic division but failed to initiate meiosis II. Instead, loss of c-mos function led to chromosome decondensation, reformation of a nucleus after meiosis I, and cleavage to two cells. Therefore, c-mos is required for meiosis II during murine oocyte maturation.
O'Keefe
S J
SJ
Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115.
Wolfes
H
H
Kiessling
A A
AA
Cooper
G M
GM
D000818:Animals; D002478:Cells, Cultured; D002843:Chromatin / Q000648:ultrastructure; D005260:Female; D008540:Meiosis / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D008845:Microinjections; D009843:Oligoribonucleotides / Q000494:pharmacology*; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects; D014176:Protein Biosynthesis; D011506:Proteins / Q000302:isolation & purification; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D017231:Proto-Oncogene Proteins c-mos; D011519:Proto-Oncogenes; D012313:RNA / Q000235:genetics*; D016372:RNA, Antisense; D012333:RNA, Messenger / Q000037:antagonists & inhibitors*
D002843:Chromatin; D009843:Oligoribonucleotides; D011506:Proteins; D011518:Proto-Oncogene Proteins; D016372:RNA, Antisense; D012333:RNA, Messenger; D012313:RNA; D017231:Proto-Oncogene Proteins c-mos