Quantitative analysis of RNA produced by slow and fast alleles of Adh in Drosophila melanogaster.
The alcohol dehydrogenase (ADH) locus (Adh) of Drosophila melanogaster in polymorphic on a world-wide basis for two allozymes, Fast and Slow. This study was undertaken to determine whether the well-established difference in ADH protein concentration between the allozymes is due to a difference in mRNA levels. RNA gel blot hybridization and an RNase protection assay were used to quantify ADH mRNA levels. Each method used an Adh null mutant as an internal standard. Several Slow and Fast allele pairs of different geographic origins were analyzed. The results provide strong evidence that the ADH protein concentration difference is not accounted for by RNA level.
Laurie
C C
CC
Department of Genetics, North Carolina State University, Raleigh 27695.
Stam
L F
LF
D000426:Alcohol Dehydrogenase / Q000235:genetics*; D000483:Alleles; D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics*; D006579:Heterozygote; D009154:Mutation; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D012313:RNA / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D012260:Ribonucleases / Q000378:metabolism; D014170:Transformation, Genetic
D012333:RNA, Messenger; D012313:RNA; D000426:Alcohol Dehydrogenase; D012260:Ribonucleases