title: The effect of small intestinal transplantation on intraluminal levels of serotonin and substance P.
abstract: This study was performed to examine the effect of transplantation, and thus extrinsic denervation, of the small intestine on intraluminal release of serotonin and substance P. Heterotopic 40-cm-long proximal (jejunal) small intestinal isografts were performed in six 200- to 250-g adult male Lewis rats under general anesthesia. Bowel ends were exteriorized as ostomies. Six Lewis rats with neurovascularly intact 40-cm proximal small bowel Thiry-Vella loops exteriorized as ostomies served as the control animals. On the seventh postoperative day, the intestinal loops were perfused at 0.5 ml/min for three 10-min periods with normal saline followed by an equilibrium period and then for three 10-min periods with 20% dextrose. Perfusates were collected for each period and levels of serotonin and substance P were determined by radioimmunoassay. Intraluminal serotonin levels rose from 29 +/- 9 ng/ml during saline perfusion to 115 +/- 28 ng/ml during intestinal perfusion with 20% dextrose in the innervated loops and from 21 +/- 7 ng/ml to 94 +/- 26 ng/ml in the transplanted loops. While there was a statistically significant increase in mean intraluminal serotonin levels following perfusion with 20% dextrose in both the control and transplant groups, there was no difference in the intraluminal serotonin response between controls and transplant recipients. In contrast, 20% dextrose had no effect on luminal release of substance P in either group. These results indicate that extrinsic denervation of the small intestine has no effect on the intraluminal serotonin response to stimulation and suggest that serotonin and substance P are not released into the intestinal lumen by the same regulatory mechanisms.
authors: Department of Surgery, State University of New York-Health Science Center, Brooklyn 11203.
authors: C A
authors: CA
authors: LaRosa
authors: K
authors: K
authors: Kimura
authors: L S
authors: LS
authors: Dresner
authors: E
authors: E
authors: Birnbaum
authors: B M
authors: BM
authors: Jaffe
mesh_terms: D000818:Animals; D007421:Intestine, Small / Q000378:metabolism / Q000637:transplantation*; D008297:Male; D009994:Osmolar Concentration; D010477:Perfusion / Q000295:instrumentation; D051381:Rats; D011917:Rats, Inbred Lew; D012701:Serotonin / Q000378:metabolism*; D013373:Substance P / Q000378:metabolism*
chemical_list: D012701:Serotonin; D013373:Substance P