High-performance liquid chromatography of the anti-tumour agent triethylenethiophosphoramide and its metabolite triethylenephosphoramide with sodium sulphide, taurine and o-phthalaldehyde as pre-column fluorescent derivatization reagents.
The method described is based on the reaction of triethylenethiophosphoramide (ThioTEPA) and triethylenephosphoramide (TEPA), through their ethyleneimine groups, with sodium sulphide, taurine and o-phthalaldehyde to give fluorescent products, and separation of the derivatives by reversed-phase high-performance liquid chromatography. The method was successfully applied to the determination of ThioTEPA and TEPA in rabbit plasma samples after clean-up with an Extrelut 3 column. The recoveries of ThioTEPA and TEPA from plasma were 66.1-80.3% and the limits of determination in plasma were ca. 10 and 20 ng/ml, respectively.
Sano
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Faculty of Pharmaceutical Sciences, Science University of Tokyo, Japan.
Matsutani
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Takitani
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D000818:Animals; D001389:Azirines / Q000097:blood*; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D011817:Rabbits; D013050:Spectrometry, Fluorescence; D013440:Sulfides; D013654:Taurine; D013852:Thiotepa / Q000097:blood*; D013721:Triethylenephosphoramide / Q000097:blood*; D009764:o-Phthalaldehyde
D001389:Azirines; D007202:Indicators and Reagents; D013440:Sulfides; D013654:Taurine; D009764:o-Phthalaldehyde; D013852:Thiotepa; D013721:Triethylenephosphoramide; C033479:sodium sulfide