title: Natural killer (NK) activity and interferon (IFN) production by a fraction of spleen and blood lymphocytes in swine.
abstract: After carbonyl iron treatment and gradient isolation, spleen and blood pig lymphocytes exhibited NK activity and produced IFN after viral induction. Removal of plastic-adherent cells, including the majority of B cells, did not change these activities. The plastic-non-adherent cells were further separated into two subsets of roughly similar size by panning using a monoclonal, anti-T, and anti-null cell antibodies (81 + cells). NK activity and IFN production were found in the 81 - cell fraction. A significantly higher proportion of null lymphocytes from blood and of splenic Fc-gamma receptor-bearing lymphocytes was also found among the 81 - cell fraction as compared to the 81 + fraction, without any change among other subsets. Similar proportions of helper (PT4+), cytotoxic (PT8+) and total T cells (MSA4+) were found among lymphocytes bound to target K562 cells and among the whole lymphocyte population. In contrast, lymphocytes that bound K562 cells demonstrated a striking increase in the proportion of Fc-gamma receptor-positive cells of high affinity. These results show that NK cells and IFN-producing cells are mainly included in the same blood and spleen fraction, and suggest that among 81 - cells only those expressing an Fc-gamma receptor of high affinity are active.
authors: Laboratoire de Pathologie Porcine, INRA, Centre de Recherches de Tours, Nouzilly, France.
authors: H
authors: H
authors: Salmon
authors: B
authors: B
authors: Charley
authors: C
authors: C
authors: Labonnardiere
authors: M
authors: M
authors: Olivier
authors: K
authors: K
authors: Kelley
authors: A
authors: A
authors: Paraf
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002469:Cell Separation; D007372:Interferons / Q000096:biosynthesis*; D007694:Killer Cells, Natural / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D011961:Receptors, Fc; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013552:Swine / Q000097:blood / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D011961:Receptors, Fc; D007372:Interferons