An indirect method for quantitation of cellular zinc content of Timm-stained cerebellar samples by energy dispersive X-ray microanalysis.
The absolute concentration of zinc in the Purkinje cells of the rat cerebellum was determined by means of energy dispersive X-ray microanalysis (EDAX). Gelatine blocks with known zinc concentrations were stained by Timm's sulphide-silver method, and their silver concentrations were measured by EDAX. A linear correlation was found between the zinc and silver concentrations and this linear function was used as a quantitative calibration for evaluation of sulphide-silver staining, after perfusion with sodium-sulphide solution, fixation with glutaraldehyde, cryostat sectioning and staining of cerebellar samples in Timm's reagent.
Farkas
I
I
Central Research Laboratory, Medical University, Szeged, Hungary.
Szerdahelyi
P
P
Kása
P
P
D000818:Animals; D002531:Cerebellum / Q000032:analysis*; D004577:Electron Probe Microanalysis; D051381:Rats; D012834:Silver; D013194:Staining and Labeling; D013440:Sulfides; D015032:Zinc / Q000032:analysis*
D013440:Sulfides; D012834:Silver; D015032:Zinc