A gag-env hybrid protein of human T-cell leukemia virus type I and its application to serum diagnosis.
A fused gene of the gag and env sequences of human T-cell leukemia virus type I (HTLV-I), the causative agent of adult T-cell leukemia, was constructed in vitro and expressed in Escherichia coli. The gag-env hybrid protein accumulated as insoluble granules with a yield of approximately 12% of the total proteins. In an enzyme-linked immunosorbent assay done with the use of the gag-env hybrid protein, all 57 seropositive sera gave positive signals, and none of the sera from normal persons did. This system can produce large quantities of the gag-env hybrid protein, which can be used for mass screening of human sera for HTLV-I infection.
Kuga
T
T
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Tokyo.
Yamasaki
M
M
Sekine
S
S
Fukui
M
M
Yokoo
Y
Y
Itoh
S
S
Yoshida
M
M
Hattori
T
T
Takatsuki
K
K
D000939:Epitopes / Q000032:analysis; D015683:Gene Products, gag; D015490:HTLV-I Infections / Q000175:diagnosis*; D006801:Humans; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000276:immunology; D011994:Recombinant Proteins / Q000096:biosynthesis*; D012191:Retroviridae Proteins / Q000096:biosynthesis* / Q000276:immunology; D012698:Serologic Tests; D014759:Viral Envelope Proteins / Q000096:biosynthesis* / Q000276:immunology
D000939:Epitopes; D015683:Gene Products, gag; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D012191:Retroviridae Proteins; D014759:Viral Envelope Proteins