The morphology of simian immunodeficiency virus as shown by negative staining electron microscopy.
Negative staining electron microscopy was used to study sucrose gradient-purified preparations of the simian immunodeficiency virus (SIVmac251). Both isolated and aggregated virus particles were observed together with some free-lying virus cores. The cores were 110 nm long and 25 to 50 nm wide and were mainly conical or wedge-like in shape. Surface projections were seen on the envelope membrane of many of the virus particles; the knobs were approximately 6 nm in length, 10 nm wide and from an end-on view they had a Y or triangular-shaped morphology.
Grief
C
C
National Institute for Biological Standards and Control, South Mimms, Hertfordshire, U.K.
Hockley
D J
DJ
Fromholc
C E
CE
Kitchin
P A
PA
D002499:Centrifugation, Density Gradient; D008854:Microscopy, Electron / Q000379:methods; D009942:Organometallic Compounds; D010316:Particle Size; D015302:Simian Immunodeficiency Virus / Q000648:ultrastructure*; D013194:Staining and Labeling / Q000379:methods; D014759:Viral Envelope Proteins / Q000032:analysis; D014771:Virion / Q000648:ultrastructure
D009942:Organometallic Compounds; D014759:Viral Envelope Proteins; C005460:uranyl acetate