Evidence from dithizone and selenium zinc histochemistry that perivascular mossy fiber boutons stain preferentially "in vivo".
This paper describes a perivascular staining pattern that is obtained when dithizone or sodium selenite are used to label zinc intravitally. Our observations indicate that the perivascular staining is a result of zinc labeling in mossy fiber boutons adjacent to capillaries and suggest that there might be a special blood brain barrier in the mossy fiber regions.
Howell
G A
GA
Laboratory for Neurobiology, University of Texas, Dallas 75080.
Frederickson
C J
CJ
Danscher
G
G
D000818:Animals; D001369:Axons / Q000032:analysis; D001391:Azo Compounds; D001812:Blood-Brain Barrier; D002196:Capillaries / Q000032:analysis; D002415:Cats; D004230:Dithizone; D005260:Female; D006624:Hippocampus / Q000032:analysis / Q000648:ultrastructure*; D006651:Histocytochemistry; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D009412:Nerve Fibers / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains; D012643:Selenium; D013194:Staining and Labeling / Q000379:methods; D015032:Zinc / Q000032:analysis*
D001391:Azo Compounds; D004230:Dithizone; D012643:Selenium; D015032:Zinc