Inosiplex enhances the growth of Epstein-Barr nuclear antigen-positive B-cells from patients with rheumatoid arthritis and infectious mononucleosis.
The in vitro effects of inosiplex (INO) on proliferation of Epstein-Barr virus (EBV)-carrying B-cells were evaluated. As measured by 3H-thymidine uptake, INO significantly augmented the proliferation of long-term cultured Epstein-Barr nuclear antigen (EBNA)-positive B-cells which were obtained from patients with rheumatoid arthritis (RA) and infectious mononucleosis (IM). In co-culture experiments, INO and anti-immunoglobulin M antibodies coupled to sepharose (anti-mu) had additive effects on proliferation of EBNA-positive B-cells. We conclude that its direct effects on B-cell function should be considered when INO is used for the treatment of diseases characterized by B-cell hyperreactivity.
Becker
H
H
III. Medizinische Klinik, Universität Giessen, F.R.G.
Helmke
K
K
D000293:Adolescent; D000328:Adult; D000368:Aged; D000956:Antigens, Viral / Q000032:analysis*; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000382:microbiology; D002478:Cells, Cultured; D019309:Epstein-Barr Virus Nuclear Antigens; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000276:immunology; D007244:Infectious Mononucleosis / Q000276:immunology*; D007288:Inosine / Q000031:analogs & derivatives*; D007542:Inosine Pranobex / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D008875:Middle Aged
D000956:Antigens, Viral; D019309:Epstein-Barr Virus Nuclear Antigens; D007075:Immunoglobulin M; D007288:Inosine; D007542:Inosine Pranobex