Molecular cloning of cDNAs corresponding to two genes of alpha 1-acid glycoprotein and characterization of two alleles of AGP-1 in the mouse.
cDNAs corresponding to two types of mouse alpha 1-acid glycoproteins (AGPs), Agp-1 and Agp-2, were cloned and sequenced. Two alleles of Agp-1 were also identified from inbred mice by restriction site polymorphisms. One allele, Agp-1A, is associated with AKR/J, SJL/J, and SWR/J mice, while the other, Agp-1B, is found in BALB/c, DBA/2, C57BL/6, B10, and A/J mice. cDNAs of both Agp-1A and Agp-1B were cloned from BALB/c and (B10 x AKR/J)F1 mice. A single nucleotide substitution in the coding sequence of Agp-1 is responsible for the observed difference in electrophoretic mobilities of Agp-1A and Agp-1B. Several additional nucleotide variations in the 3' untranslated region were also found. In contrast to these two alleles, substantial nucleotide difference exist between Agp-1 and Agp-2. Agp-1 and Agp-2 contain five and six potential N-glycosylation sites, respectively.
Lee
S C
SC
Institute of Biological Chemistry, Academia Sinica, National Taiwan University, Taipei.
Chang
C J
CJ
Lee
Y M
YM
Lei
H Y
HY
Lai
M Y
MY
Chen
D S
DS
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular / Q000379:methods; D004247:DNA / Q000235:genetics; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009961:Orosomucoid / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012313:RNA / Q000302:isolation & purification
D009961:Orosomucoid; D012313:RNA; D004247:DNA