title: A lead conversion method for staining thiamine pyrophosphatase and other phosphatases in rat brain after thin layer polyacrylamide gel isoelectric focusing.
abstract: Rat brain thiamine pyrophosphatase (TPPase) was separated by thin layer polyacrylamide gel isoelectric focusing (IF) and stained with a modification of the lead conversion method of Allen and Hyncik (1963). 10 bands with TPPase activity were observed in the pH range 4.6-7.1. The overall IF pattern of TPPase was similar to that of uridine diphosphatase and inosine diphosphatase but was clearly different from that of adenosine diphosphatase, p-nitrophenyl phosphatase, alpha-naphthyl phosphatase and thiamine monophosphatase. A semiquantitative assessment of TPPase isoenzymes has been performed using laser densitometry.
authors: Clinica Neurologica I, Università di Milano, Italy.
authors: P
authors: P
authors: Mazzarello
authors: M
authors: M
authors: Poloni
authors: C
authors: C
authors: Patrini
authors: D
authors: D
authors: Bosone
authors: G
authors: G
authors: Rindi
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology*; D005260:Female; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis*; D007854:Lead; D008297:Male; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis*; D011755:Pyrophosphatases / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013194:Staining and Labeling; D013834:Thiamine Pyrophosphatase / Q000032:analysis*
chemical_list: D007527:Isoenzymes; D007854:Lead; D010744:Phosphoric Monoester Hydrolases; D011755:Pyrophosphatases; D013834:Thiamine Pyrophosphatase