Quantitative computer analysis of Golgi-impregnated neurons using a new 'expanded stick' model for coding; its application to the study of axons.
A computer program to obtain quantitative data from an expanded 'stick model' of the Golgi-impregnated neuron is described. The computer program allows complete three-dimensional morphometric analysis of neurons, including the size of varicosities and spines and their distribution on neuronal branches. Furthermore, the perimeter and area of the perikaryon as well as the length, width and area of neuronal processes can be obtained. The use of this computer program is illustrated by coding two types of axons.
Freire
M
M
Instituto Cajal, Madrid, Spain.
D000818:Animals; D001369:Axons / Q000648:ultrastructure*; D002540:Cerebral Cortex / Q000648:ultrastructure; D006735:Horseradish Peroxidase; D007091:Image Processing, Computer-Assisted; D051379:Mice; D008838:Microcomputers; D009474:Neurons / Q000648:ultrastructure*; D012984:Software; D013194:Staining and Labeling
D006735:Horseradish Peroxidase