title: Demonstration of cytokines in biological medicines produced in mammalian cell lines.
abstract: Commercially produced biological medicines may contain cytokines secreted by mammalian cell lines. Several such cell lines were found to produce interleukin 6 and, after stimulation, to secrete interleukin 1, tumour necrosis factor, granulocyte colony-stimulating factor, and granulocyte-macrophage colony-stimulating factor. High levels of interleukin 6 were detected in several vaccines and rDNA-derived proteins, and certain vaccines contained interleukin 1, granulocyte colony-stimulating factor, and granulocyte-macrophage colony-stimulating factor. Some preparations of human monoclonal antibodies were also found to contain interleukin 1 and tumour necrosis factor. Cytokines may contribute to certain types of adverse reactions to these products.
authors: Division of Immunobiology, National Institute for Biological Standards and Control, South Mimms, Potters Bar, Hertfordshire.
authors: A J
authors: AJ
authors: Gearing
authors: J E
authors: JE
authors: Cartwright
authors: C R
authors: CR
authors: Bird
authors: M
authors: M
authors: Wadhwa
authors: R
authors: R
authors: Priest
authors: R
authors: R
authors: Thorpe
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D001685:Biological Factors / Q000009:adverse effects; D002461:Cell Line, Transformed; D003115:Colony-Stimulating Factors / Q000032:analysis; D016207:Cytokines; D004247:DNA / Q000032:analysis*; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006133:Growth Substances / Q000032:analysis; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007375:Interleukin-1 / Q000032:analysis; D015850:Interleukin-6 / Q000032:analysis; D008722:Methods; D051379:Mice; D011819:Rabies Vaccines / Q000032:analysis; D011994:Recombinant Proteins / Q000032:analysis; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis; D014765:Viral Vaccines / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D001685:Biological Factors; D003115:Colony-Stimulating Factors; D016207:Cytokines; D006133:Growth Substances; D007074:Immunoglobulin G; D007375:Interleukin-1; D015850:Interleukin-6; D011819:Rabies Vaccines; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D014765:Viral Vaccines; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D004247:DNA