title: The use of cryostat microtomy in a simplified Golgi method for staining vertebrate neurons.
abstract: The use of cryostat and cryoprotective measures for processing Golgi impregnated brain tissue has shortened and simplified the method without loss of quality. The procedure contains the following steps: (1) the animal is perfused with phosphate-buffered paraformaldehyde and the brain removed for storage in the fixative; (2) the brain is rinsed in buffer, cut into 3- to 6-mm-thick blocks and placed in Ramon-Moliner's impregnation solution for 2-4 weeks; (3) the brain tissue is cryoprotected by soaking in 30% sugar for 24 h and then frozen and cut on a cryostat, the sections, being collected directly on slides; and (4) the mounted sections are then alkalized, fixed, rinsed, counterstained and rinsed again before dehydration, clearing and coverslipping.
authors: Institute of Marine Science, University of Alaska Fairbanks, Fairbanks 99775-1080.
authors: S O
authors: SO
authors: Ebbesson
authors: M
authors: M
authors: Cheek
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology*; D005629:Frozen Sections; D008867:Microtomy; D009474:Neurons / Q000648:ultrastructure; D012834:Silver; D013194:Staining and Labeling / Q000379:methods*; D014714:Vertebrates
chemical_list: D012834:Silver