Monoclonal antibody GB24 recognizes a trophoblast-lymphocyte cross-reactive antigen.
GB24 is a mouse monoclonal antibody (IgG1) raised against human term placental microvilli. This antibody displayed pan-trophoblast reactivity pattern. In addition, GB24 recognized normal peripheral leukocytes and transformed cell lines (Daudi, HL-60, Jurkat, AV3, BeWo, HT-29) by using membrane immunofluorescence and flow cytometry. By SDS-polyacrylamide gel electrophoresis, this antibody immunoprecipitated two proteins of 62 kilodaltons (kDa) and 75 kDa from placental microvilli. Isoelectrofocusing analysis revealed that these two bands exhibited different isoelectric points: 4.7 for the 62 kDa protein, 4.6 and 4.4 for the two spots corresponding to the 75 kDa protein. Microvilli from different placentae revealed substantial differences regarding the intensity of the labeling of these two bands, suggesting that the antigens recognized by GB24 are probably allotypic.
Hsi
B L
BL
INSERM U210, Faculté de Médecine, Nice, France.
Yeh
C J
CJ
Fénichel
P
P
Samson
M
M
Grivaux
C
C
D000650:Amnion / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000145:classification; D001324:Autoantigens / Q000276:immunology* / Q000302:isolation & purification; D001667:Binding, Competitive; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis; D005260:Female; D006801:Humans; D007519:Isoantigens; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D010920:Placenta / Q000276:immunology; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000276:immunology / Q000302:isolation & purification; D014327:Trophoblasts / Q000276:immunology*
D000911:Antibodies, Monoclonal; D001324:Autoantigens; D000939:Epitopes; D007519:Isoantigens; D011257:Pregnancy Proteins; C055067:trophoblast-lymphocyte cross-reactive antigens