Cytotoxic T lymphocyte recognition of novel allodeterminants expressed on in vitro selected H-2Kb mutants.
In the present study, in vitro derived H-2Kb mutants have been examined by alloreactive CTL. Two mutants, R8.24 and R8.246, have been shown to express novel determinants detected by CTL generated against some but not all in vivo derived Kb mutants. BDF1 anti-bm3, anti-bm11, anti-bm19, anti-bm23, and anti-bm6 CTL populations lyse the two R8 variants. The novel determinants expressed on the R8 mutants detected by the bm3 and bm23-specific CTL appear to differ from the determinant recognized by the bm6-specific CTL. No new serologically defined determinants were detected on any of 18 independent R8 variants. However, these results do not rule out the existence of new determinants which could be recognized by antibodies. Finally, the relationship between the T cell recognition of the in vivo and in vitro derived mutants and their use in understanding the structure/function relationships between the immune response and class I Ag based on recent crystallographic analyses is discussed.
Lewis
J
J
Transplantation Biology Section, National Cancer Institute, Bethesda, MD 20892.
Foo
M
M
Geier
S S
SS
Kumar
P A
PA
Nathenson
S G
SG
Bluestone
J A
JA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000502:physiology; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D006183:H-2 Antigens / Q000235:genetics* / Q000276:immunology; D064532:Histocompatibility Antigen H-2D; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006183:H-2 Antigens; D064532:Histocompatibility Antigen H-2D