[Chromatographic separation of activated proteases from human plasma].
After separation of aceton and dextran sulfate activated human plasma by column chromatography on DEAE-cellulose three esterolytically and amidolytically active fractions, respectively, were obtained, which were assigned to the following species: plasma kallikrein (PK), PK.alpha-macroglobulin.HMW-Kininogen. Their percentage in the whole activity is variable. The proportion of free PK is low (0.11). For characterization of the products we studied inhibition by different polyvalent inhibitors. The Michaelis constant (Km) with p-toluene-sulfonyl-L-arginine methyl ester (TAME) were determined. For simulation of in vivo conditions dextran sulfate activated plasma was inactivated at 37 degrees C. The residual activity and the spontaneous activity in plasma from patients with shock are produced by different active protease inhibitor complexes.
Lehmann
B
B
Medizinische Akademie Carl Gustav Carus Dresden.
Taucher
M
M
Kühne
H
H
Scheuch
D W
DW
D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D004789:Enzyme Activation; D006801:Humans; D007610:Kallikreins / Q000097:blood / Q000302:isolation & purification; D007700:Kinetics; D007704:Kininogens / Q000097:blood / Q000302:isolation & purification; D010447:Peptide Hydrolases / Q000097:blood* / Q000302:isolation & purification; D000511:alpha-Macroglobulins / Q000097:blood
D007704:Kininogens; D000511:alpha-Macroglobulins; D010447:Peptide Hydrolases; D007610:Kallikreins
Zur chromatographischen Trennung aktivierter Proteasen aus menschlichem Plasma.