Cytokeratins in hamster cheek pouch epithelium during DMBA-induced carcinogenesis.
The pattern of keratin expression in hamster cheek pouch epithelium during 15-wk of DMBA-induced carcinogenesis was studied. The sequential changes in cytokeratins of premalignant and malignant tissues and comparative investigation of normal epithelial tissues were examined during a weekly sequential DMBA-induced chemical carcinogenesis. Keratin polypeptides of normal pouch epithelium appear in a molecular weight range of 43-67 kd and 5-6 proteins can be identified. The disappearance of high molecular weight keratin (61-67 kd) was observed from the 6-wk DMBA-treated premalignant group to the 15-wk DMBA-treated malignant group. An additional keratin polypeptide was noted initially on the 11th-wk-DMBA-treated group and remained to the 15th-wk-DMBA treated group.
Lin
L M
LM
Oral Pathology and Diagnosis Department, School of Dentistry, Kaohsiung Medical College, Taiwan R.O.C.
Chen
Y K
YK
Huang
Y L
YL
Mostofi
R
R
Toto
P
P
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D002610:Cheek / Q000032:analysis / Q000473:pathology; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D004848:Epithelium / Q000032:analysis / Q000473:pathology; D015151:Immunoblotting; D007633:Keratins / Q000032:analysis* / Q000145:classification; D008297:Male; D008647:Mesocricetus; D009061:Mouth Mucosa / Q000032:analysis* / Q000473:pathology; D009062:Mouth Neoplasms / Q000032:analysis* / Q000473:pathology; D010159:Palate / Q000032:analysis; D011230:Precancerous Conditions / Q000032:analysis* / Q000473:pathology; D012867:Skin / Q000032:analysis; D012967:Sodium Dodecyl Sulfate
D012967:Sodium Dodecyl Sulfate; D015127:9,10-Dimethyl-1,2-benzanthracene; D007633:Keratins