Detection of pseudorabies virus transcripts in trigeminal ganglia of latently infected swine.
Two pseudorabies virus (PRV) poly(A)+ RNAs, 2.0 and 0.95 kilobases, were detected in the trigeminal ganglia of latently infected pigs. These RNAs were partially colinear at the 3' end. They were located in the BamHI J fragment (0.706 to 0.737 map units), 3' of the PRV immediate-early gene. A potential polyadenylation signal was also identified in BamHI-J. The direction of transcription of the latency-associated RNAs was antiparallel (i.e., opposite in polarity) to that of the PRV immediate-early mRNA.
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National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa 50010.
D000818:Animals; D015152:Blotting, Northern; D005814:Genes, Viral; D011558:Herpesvirus 1, Suid / Q000235:genetics / Q000302:isolation & purification*; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification*; D011557:Pseudorabies / Q000382:microbiology*; D012313:RNA / Q000235:genetics / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D015183:Restriction Mapping; D013552:Swine; D014158:Transcription, Genetic; D012668:Trigeminal Ganglion / Q000382:microbiology*; D014276:Trigeminal Nerve / Q000382:microbiology*
D012333:RNA, Messenger; D012367:RNA, Viral; D011061:Poly A; D012313:RNA