Elucidation of conservative elements of calmodulin-dependent enzymes with the use of monoclonal antibodies.
Monoclonal antibodies against human erythrocyte membrane Ca2+-ATPase were obtained. The binding of monoclonal antibodies to the enzyme resulted in a decrease in the enzyme sensitivity to calmodulin (CaM). The effects of monoclonal antibodies on other CaM-dependent enzymes, namely, on the phosphodiesterase of cAMP, phosphorylase kinase, and Ca2+-CaM-dependent protein kinase II (PK II), were studied. It was found that all four enzymes contain a common antigenic site. However, the inhibitory effect of antibodies was observed only with respect to Ca2+-ATPase and PK II. The kinetics of the binding of monoclonal antibodies and their inhibitory action were investigated. It was shown that the antigenic site is confined to the calmodulin-binding portion of Ca2+-ATPase and PK II.
Alakhov VYu
Institute of Applied Molecular Biology, USSR Ministry of Health, Moscow.
Modyanov
N N
NN
Shakhparonov
M I
MI
Zvaritch
E I
EI
Feschenko
M S
MS
Lutzenko
S V
SV
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D000252:Calcium-Transporting ATPases / Q000097:blood* / Q000276:immunology; D002147:Calmodulin / Q000494:pharmacology*; D054677:Cyclic Nucleotide Phosphodiesterases, Type 1; D000939:Epitopes / Q000276:immunology; D004910:Erythrocyte Membrane / Q000201:enzymology; D006801:Humans; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D010764:Phosphorylase Kinase / Q000276:immunology / Q000378:metabolism*; D011494:Protein Kinases / Q000276:immunology / Q000378:metabolism*; D011863:Radioimmunoassay
D000911:Antibodies, Monoclonal; D002147:Calmodulin; D000939:Epitopes; D011494:Protein Kinases; D010764:Phosphorylase Kinase; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D054677:Cyclic Nucleotide Phosphodiesterases, Type 1; D000252:Calcium-Transporting ATPases