title: The high molecular weight and the low molecular weight acid phosphatases of the frog liver and their phosphotyrosine activity.
abstract: 1. Two molecular weight classes of non-specific acid phosphatases (AcPases) (3.1.3.2) are present in the frog (Rana esculenta) liver: a higher molecular weight (HMW) of Mr 140,560 and a lower molecular weight (LMW) of Mr 38,180 enzyme. 2. The LMW AcPase was described earlier and the HMW AcPase of optimum pH 4.8 is shown to be a L(+)-tartrate sensitive, thermolabile, dimeric glycoenzyme slightly activated by DTT. 3. The HMW and the LMW AcPases exhibit activity for phosphotyrosine which showed similar sensitivity to various effectors as the p-nitrophenyl phosphatase activity; however, both enzymes differed substantially in this respect suggesting that they might be involved in different metabolic steps.
authors: Institute of Biochemistry, University of Wrocław, Poland.
authors: H
authors: H
authors: Jańska
authors: A
authors: A
authors: Kubicz
authors: A
authors: A
authors: Szalewicz
authors: J
authors: J
authors: Haraźna
mesh_terms: D000135:Acid Phosphatase / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004229:Dithiothreitol / Q000494:pharmacology; D007527:Isoenzymes / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D019000:Phosphotyrosine; D011893:Rana esculenta; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D007527:Isoenzymes; D019000:Phosphotyrosine; D014443:Tyrosine; D000135:Acid Phosphatase; D004229:Dithiothreitol