title: A specific solid-phase assay for the detection of immune complexes containing Entamoeba histolytica antigens.
abstract: An assay specific for immune complexes containing Entamoeba histolytica antigens has been developed. Rabbit antibody specific for human gamma globulin is attached to a solid phase (nitrocellulose). During the first incubation step polyethylene glycol-precipitated immune complexes are bound to the rabbit antibody attached to the solid phase. In the second incubation step a radiolabelled rabbit antibody specific for Entamoeba histolytica combines with E. histolytica antigens in the complexes. Quantitation of the radiolabel provides a direct measurement of the level of specific immune complexes. The principle of the method has been verified using artificial soluble immune complexes prepared by mixing a pool of serum containing a high titre of anti-E. histolytica antibodies (greater than 1:2048 by hemagglutination assay) with the E. histolytica antigen. This antigen was prepared from axenically cultured NIH:200 strains of Entamoeba histolytica. Using sera from patients with clinical amoebic disease this method detected immune complexes containing E. histolytica in a high proportion of cases.
authors: Kothari Centre of Gastroenterology, CMRI, Calcutta, India.
authors: A
authors: A
authors: Mohimen
authors: T K
authors: TK
authors: Maitra
authors: K N
authors: KN
authors: Jalan
authors: S
authors: S
authors: Mehra
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan; D000936:Antigen-Antibody Complex / Q000032:analysis*; D000953:Antigens, Protozoan / Q000032:analysis*; D001798:Blood Proteins / Q000032:analysis; D003101:Collodion; D004748:Entamoeba histolytica / Q000276:immunology*; D000939:Epitopes / Q000032:analysis; D005243:Feces / Q000032:analysis; D006801:Humans; D008101:Liver Abscess, Amebic / Q000097:blood / Q000175:diagnosis; D010047:Ovalbumin / Q000032:analysis; D011863:Radioimmunoassay / Q000379:methods
chemical_list: D000913:Antibodies, Protozoan; D000936:Antigen-Antibody Complex; D000953:Antigens, Protozoan; D001798:Blood Proteins; D000939:Epitopes; D003101:Collodion; D010047:Ovalbumin