title: The effect of 3T3 fibroblasts on the expression of anchorage independence and cornification of oral keratinocytes.
abstract: This study examined the effect of 3T3 fibroblasts on the expression of anchorage independence and the degree of cornification in early cultures of three carcinoma-derived epithelial cell lines (R59, R63a, R63b) and in one cell line derived from non-malignant dysplastic epithelium where there was no evidence of invasion (R66a). The epithelial cell lines originated from the palatal (R63a, R66a) and the lingual (R59, R63b) mucosa of rats that had been painted with the carcinogen 4-nitroquinoline N-oxide. In the absence of 3T3 fibroblasts, progressive culture resulted in an increase in the colony forming efficiency (CFE) of R63a, R63b and R59 and a decrease in the percentage of cornified cells in all cell lines. 3T3 fibroblasts caused a decrease in the CFE and the degree of cornification in the 3T3-dependent cell line (R63a), particularly at the lower passages, but these parameters remained essentially unchanged by 3T3 fibroblasts in the 3T3-independent cell lines (R59, R63b). 3T3 fibroblasts did not influence the cornification of R66a and this cell line remained anchorage dependent throughout the study. The results suggest that in malignant cell lines characterised by being independent of 3T3 fibroblasts (R63b, R59) the CFE was inversely correlated to the degree of cornification. However, in the malignant cell line showing a greater dependence on support (R63a) the relationship between CFE and cornification was unclear because these parameters may have been modulated by the presence of 3T3 fibroblasts. The cell line from dysplastic non-invasive tissue (R66a) differed from its malignant counterparts in the fact that CFE and cornification were unaffected by 3T3 fibroblasts despite previous studies showing a dependence on mesenchymal support.
authors: University Department of Oral Medicine, Surgery and Pathology, Bristol Dental Hospital, England.
authors: J
authors: J
authors: Luker
authors: I J
authors: IJ
authors: Crane
authors: C
authors: C
authors: Scully
authors: S S
authors: SS
authors: Prime
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D000078404:Epidermal Cells; D004847:Epithelial Cells; D005347:Fibroblasts / Q000502:physiology*; D007633:Keratins; D008297:Male; D009061:Mouth Mucosa / Q000166:cytology*; D009062:Mouth Neoplasms / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007633:Keratins