Identification, cloning, and characterization of an immune activation gene.
We have identified an immune activation gene, denoted Act-2, by differential hybridization screening of an activated T-cell library. The gene is induced rapidly after T-cell activation with phytohemagglutinin, B-cell activation with Staphylococcus aureus Cowan I, and monocyte activation with lipopolysaccharide. We have isolated a cDNA containing the full-length coding region. The deduced amino acid sequence predicts an open reading frame of 92 amino acids, including a very hydrophobic N terminus, which by weight matrix score is predicted to be a signal peptide. Using a baculovirus expression system, we have shown that this gene encodes a secreted product. It is therefore possible that Act-2 represents a newly discovered cytokine.
Lipes
M A
MA
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892.
Napolitano
M
M
Jeang
K T
KT
Chang
N T
NT
Leonard
W J
WJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis; D005802:Genes, MHC Class II; D006801:Humans; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D010835:Phytohemagglutinins / Q000494:pharmacology; D014176:Protein Biosynthesis; D012313:RNA / Q000378:metabolism; D016372:RNA, Antisense; D013601:T-Lymphocytes
D010835:Phytohemagglutinins; D016372:RNA, Antisense; D012313:RNA; D004247:DNA