Evidence for the identity of hirudin isolated after kidney passage with the starting material.
Natural, sulfated and recombinant, non-sulfated hirudin preparations re-isolated from urine after kidney passage were compared with the corresponding administered preparations by reversed-phase high-performance liquid chromatography, and by amino acid sequence and composition analysis. It could be demonstrated that the hirudins were excreted in unmodified form. Natural hirudin could be fractionated by the chromatographic system used into a large number of isoinhibitor forms, some of which were shown to differ from each other in their amino acid sequence.
Henschen
A
A
Max-Planck-Institute for Biochemistry, Martinsried/Munich, FRG.
Markwardt
F
F
Walsmann
P
P
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D006629:Hirudins / Q000302:isolation & purification* / Q000493:pharmacokinetics / Q000652:urine; D007668:Kidney / Q000378:metabolism*
D000596:Amino Acids; D006629:Hirudins