Effect of 5-azacytidine on gene expression in marrow stromal cells.
When exposed to 5-azacytidine, marrow stromal cells from active long-term marrow cultures and cell lines derived from simian virus 40-transformed stromal cells rapidly upregulated c-abl and interleukin-6 transcripts while downregulating the expression of collagen I, a major matrix protein. Similar effects occurred with interleukin-1 alpha and tumor necrosis factor alpha, although the time course was considerably prolonged.
Andrews
D F
DF
Medical Service, VA Medical Center, Seattle, Washington 98108.
Nemunaitis
J
J
Tompkins
C
C
Singer
J W
JW
D001374:Azacitidine / Q000494:pharmacology*; D015152:Blotting, Northern; D001853:Bone Marrow / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis / Q000235:genetics; D004247:DNA / Q000187:drug effects; D015342:DNA Probes; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D015850:Interleukin-6; D007378:Interleukins / Q000096:biosynthesis; D008745:Methylation; D010957:Plasmids; D011519:Proto-Oncogenes / Q000187:drug effects; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D015342:DNA Probes; D007375:Interleukin-1; D015850:Interleukin-6; D007378:Interleukins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D003094:Collagen; D004247:DNA; D001374:Azacitidine