[The use of ribonucleic acid as a marker for measuring microbial protein yield in the rumen. 1. Chemical determination of ribonucleic acid].
The determination of ribonucleic acid (RNA) in the duodenal digesta and the rumen microbes was carried out colorimetrically with orcinol after extraction with hot NaCl solution, precipitation with phosphotungstic acid and alkaline hydrolysis. 96 +/- 1% of the RNA supplemented to the digesta content was recovered (y +/- s, n = 5). The methodic error (repetition accuracy) was 1.1%. The relation of RNA-N:N (mg/g) in the bacteria mass amounted to 93.8 +/- 3.99 (y +/- s(-y), n = 48) and to 16.1 +/- 0.8 (y +/- s(-y), n = 51) mg RNA/g DM in the duodenal content.
Schönhusen
U
U
Forschungszentrum für Tierproduktion Dummerstorf-Rostock, Bereich Tierernährung Oskar Kellner, Akademie der Landwirtschaftswissenschaften der DDR.
Voigt
J
J
Piatkowski
B
B
D000818:Animals; D001419:Bacteria / Q000235:genetics*; D002417:Cattle / Q000378:metabolism*; D003124:Colorimetry; D004386:Duodenum / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012329:RNA, Bacterial / Q000032:analysis*; D012417:Rumen / Q000032:analysis* / Q000382:microbiology
D011506:Proteins; D012329:RNA, Bacterial
Untersuchungen zur Nutzung von Ribonukleinsäure als Marker für die Messung des mikrobiellen Proteinertrages im Pansen. 1. Mitteilung. Chemische Bestimmung von Ribonukleinsäure.