title: Sequence of a human keratin 13 specific cDNA encompassing coil 1B through the 3' end.
abstract: An expression library established in lambda gt11 with cDNA from squamous epithelium of the human upper digestive tract was screened with an antibody raised against keratin 13. A 1.2 kb fragment from the most strongly reacting plaque was sequenced and compared to known type I keratin sequences. The highest degree of homology was detected with the murine 47K type I keratin, which we consider to be the counterpart of human keratin 13. Tryptic peptides of keratin 13 were separated on a HPLC column and one peptide was sequenced. The amino acid sequence obtained supports the identity of the cDNA. An eight codon motif has been tandemly repeated in the C-domain of keratin 13. In spite of substantial divergence by point mutations and deletions, the remaining sequence homologies suggest that the C-domains of both the human keratin 13 and the orthologous murine protein have originated from a common ancestor.
authors: Klinik und Poliklinik für Hals-, Nasen- und Ohrenkranke, Universitätsklinikum Grosshadern, München, Federal Republic of Germany.
authors: P
authors: P
authors: Schulz
authors: E
authors: E
authors: Wachter
authors: K
authors: K
authors: Hochstrasser
authors: A G
authors: AG
authors: Wild
authors: D
authors: D
authors: Mischke
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology; D006801:Humans; D007633:Keratins / Q000235:genetics* / Q000648:ultrastructure; D051379:Mice; D008969:Molecular Sequence Data; D014066:Palatine Tonsil / Q000166:cytology / Q000502:physiology*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D007633:Keratins; D004247:DNA