[A comparison of radioreceptor assay and immunocytochemical staining for estrogen receptor in leiomyoma and myometrium].
Estrogen receptor (ER) content in myoma and myometrium was determined by radioreceptor assay (RRA), and ER staining was performed by immunocytochemical staining, i.e., the peroxidase-antiperoxidase (PAP) method, in uterine samples from eight patients with uterine leiomyoma. ER content in myoma tended to be higher than that in myometrium, but the difference was not significant. The distribution pattern of ER staining was thought to be an important criterion. When the data were compared on the basis of the number of cells in the site where the largest number of positive cells aggregated, the number for myoma was significantly higher than that for myometrium (p less than 0.05). These results suggest that myoma contains more ER positive cells and more efficiently absorbs estrogen than myometrium.
Ohtsuka
H
H
Department of Obstetrics and Gynecology, School of Medicine, University of Occupational and Environmental Health, Fukuoka.
Shinohara
M
M
Kashimura
M
M
Yoshida
K
K
Okamura
Y
Y
D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007889:Leiomyoma / Q000032:analysis*; D009215:Myometrium / Q000032:analysis*; D011869:Radioligand Assay; D011960:Receptors, Estrogen / Q000032:analysis*; D013194:Staining and Labeling; D014594:Uterine Neoplasms / Q000032:analysis*
D011960:Receptors, Estrogen