Isolation and characterization of expression cDNA clones encoding antigens of Onchocerca volvulus infective larvae.
The isolation of recombinant cDNA clones expressing antigens found in Onchocerca volvulus infective larvae is described. To isolate such clones, an expression cDNA library constructed from adult O. volvulus RNA was screened with antiserum raised against infective larvae. One clone, designated lambda RAL-1 was characterized further. The recombinant antigen produced by lambda RAL-1 stimulates proliferation of peripheral blood mononuclear cells from O. volvulus infected humans. Lambda RAL-1 is derived from a 1450 bases message that encodes a protein with an apparent molecular weight of 42,000 in adult O. volvulus. The inserted DNA of lambda RAL-1 contains an open reading frame of 1008 bp. The amino acid sequence predicted by this open reading frame contains three repeats of the sequence KKPEDWD. The identification of clones such as lambda RAL-1 will provide quantities of purified antigens sufficient to begin to study the immune response to and explore the development of immunity against the infectious form of the parasite.
Unnasch
T R
TR
Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.
Gallin
M Y
MY
Soboslay
P T
PT
Erttmann
K D
KD
Greene
B M
BM
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D001483:Base Sequence; D002458:Cell Fractionation; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D000939:Epitopes / Q000235:genetics; D006801:Humans; D007814:Larva / Q000166:cytology / Q000235:genetics / Q000276:immunology; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D009854:Onchocerca / Q000235:genetics* / Q000254:growth & development / Q000276:immunology; D009855:Onchocerciasis / Q000235:genetics / Q000276:immunology*; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D011817:Rabbits
D000947:Antigens, Helminth; D000939:Epitopes; D004247:DNA