Establishment of antigen-specific and MHC-restricted B cell hybridoma clones: a model for studying antigen presentation to T cell lines and clones.
Presentation of GAT by GAT-specific hybridoma B cell clones to GAT-specific T cell lines and clones was investigated. B cell clones expressed surface IgG2b, Ia and Fc receptors, and did not secrete immunoglobulins. The clones formed rosettes with GAT-SRBC which could be inhibited by monoclonal antibodies against GAT idiotype. Presentation of GAT by the clones was MHC-restricted and highly efficient, i.e., a few hundred B cells or very low concentration of GAT stimulated proliferation of GAT-specific T cell lines. The fusion partner, M12.4.5.2, clone presented GAT much less efficiently. Although GAT-specific B cell clones presented also heterologous antigen beef insulin to insulin-reactive T cell lines, they were not able to present insulin in the absence of soluble antigen. Presentation of GAT was inhibited by pretreatment of the clones with monoclonal antibodies against I-E, mouse Ig and GAT-idiotype, as well as related antigens GA and GT. The rate of antigen uptake was much shorter compared to resting B cells for cells pulsed with GAT for 4 hours presented the antigen well. The studies suggest that a membrane-associated Il-1 may be required in the presence of antigen presentation by B cells. The results also revealed that antigen-specific hybridomas can present specific antigen in part with association of surface immunoglobulins which may be involved in antigen uptake and focusing to T cells.
Zimecki
M
M
Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.
Kapp
J A
JA
Pierce
C W
CW
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D002999:Clone Cells / Q000276:immunology; D000939:Epitopes / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D008285:Major Histocompatibility Complex; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors
D000954:Antigens, Surface; D000939:Epitopes