title: Analysis of transcription control elements of the mouse myelin basic protein gene in HeLa cell extracts: demonstration of a strong NFI-binding motif in the upstream region.
abstract: Promoter elements of the mouse myelin basic protein (MBP) gene were analyzed by in vitro transcription using HeLa cell extracts. We demonstrated the MBTE (MBP transcription element), GC-box core and TATA-box elements, at -130, -93 and -34, respectively. The TATA-box was indispensable for the promoter function. The GC-box was suggested to function co-operatively with far upstream sequences including the MBTE. The MBTE was crucial to direct maximal transcription, and also functioned with a heterologous promoter irrespective of its orientation. We identified a ubiquitous binding factor which interacted specifically with the MBTE and activated transcription. Intensive foot-printing studies demonstrated that the MBTE had a NFI-binding sequence. The MBTE was considered to be one of the strongest NFI-binding motif among known cellular genes. Interestingly, similar strong NFI-binding motifs were suggested to be present in the enhancer of JC virus whose gene is expressed like the MBP gene, in the nervous system.
authors: Division of Behavior and Neurobiology, National Institute for Basic Biology, Okazaki, Japan.
authors: T
authors: T
authors: Tamura
authors: M
authors: M
authors: Miura
authors: K
authors: K
authors: Ikenaka
authors: K
authors: K
authors: Mikoshiba
mesh_terms: D000818:Animals; D001483:Base Sequence; D004252:DNA Mutational Analysis; D003850:Deoxyribonuclease I; D005786:Gene Expression Regulation; D005796:Genes; D006367:HeLa Cells / Q000032:analysis*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D004676:Myelin Basic Protein / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000302:isolation & purification*
chemical_list: D004676:Myelin Basic Protein; D014157:Transcription Factors; D003850:Deoxyribonuclease I