Synthesis of RNA oligomer using 9-fluorenylmethoxycarbonyl (Fmoc) group for 5'-hydroxyl protection.
An approach using a new combination of protecting groups in RNA oligomer synthesis is proposed, in which 5'-hydroxyl group of ribose moiety is temporarily protected with the alkaline labile 9-fluorenylmethoxycarbonyl (Fmoc) group and the 2'-hydroxyl group is protected with the acid labile 1-ethoxyethyl (EE) group. The adoption of this method presented great selectivity in removing the 5'-hydroxyl protecting group and facilitated the RNA oligomer synthesis. A RNA pentamer was synthesized by the phosphotriester method in solution.
Fukuda
T
T
Biotechnology Laboratories, Central Research Division, Takeda Chemical Industries Ltd, Osaka, Japan.
Hamana
T
T
Marumoto
R
R
D002851:Chromatography, High Pressure Liquid; D005449:Fluorenes; D006900:Hydroxylation; D007202:Indicators and Reagents; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000302:isolation & purification; D012313:RNA / Q000138:chemical synthesis*
D005449:Fluorenes; D007202:Indicators and Reagents; D009843:Oligoribonucleotides; D012313:RNA