Occurrence and characteristics of agglutination of Vibrio cholerae by serum from the eastern oyster, Crassostrea virginica.
Cell-free hemolymph (serum) of the eastern oyster, Crassostrea virginica, agglutinated Vibrio cholerae, including all O1 serovars and biovars. Seventy-nine other strains of bacteria, including 14 genera and 26 species, were not agglutinated. The A, B, and C factors of O1 antigen were not involved in agglutination. Bacterial agglutinating (BA) activity was demonstrated for oysters inhabiting different environments of the U.S. Atlantic and Gulf coasts. Oyster serum BA titers showed high individual variation. The serum component(s) involved in BA was inhibited by 80 degrees C heat, pronase, EDTA, mucin, and fetuin treatments. N-Acetylneuraminic acid (10 mg/ml) weakly inhibited BA activity. Ligands of V. cholerae were sensitive to neuraminidase and resistant to 80 degrees C and pronase. High salinities (24 and 30%) enhanced BA. Cross-adsorption tests with V. cholerae and human O+ erythrocytes indicated that BA and hemagglutinating activities may involve different serum components. These results imply that the ecology of V. cholerae in C. virginica is influenced by agglutinating activity of oyster serum.
Tamplin
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ML
Fishery Research Branch, U.S. Food and Drug Administration, Dauphin Island, Alabama 36528.
Fisher
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WS
D000371:Agglutination; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D004492:Edetic Acid / Q000494:pharmacology; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007124:Immunoenzyme Techniques; D019081:O Antigens; D010125:Ostreidae / Q000382:microbiology*; D011402:Pronase / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D014734:Vibrio cholerae / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D014871:Water Microbiology
D000942:Antigens, Bacterial; D019081:O Antigens; D012965:Sodium Chloride; D004492:Edetic Acid; D011402:Pronase