Immunological evidence for a relationship between the dendrotoxin-binding protein and the mammalian homologue of the Drosophila Shaker K+ channel.
Polyclonal antibodies were raised against two synthetic peptides from different parts of the predicted amino acid sequence of the mouse homologue (MBK1) of the Drosophila Shaker K+ channel. The antibodies recognized the toxin-binding subunit of the dendrotoxin-binding proteins from rat and bovine brain. The results suggest that the dendrotoxin-binding protein is related to the expression products of the mammalian homologue of the Shaker gene.
Rehm
H
H
Geriatric Research, Education and Clinical Center, VA Medical Center, Seattle, WA 98108.
Newitt
R A
RA
Tempel
B L
BL
D000818:Animals; D000906:Antibodies / Q000276:immunology; D015153:Blotting, Western; D002352:Carrier Proteins / Q000378:metabolism*; D002417:Cattle; D002852:Chromatography, Ion Exchange; D004330:Drosophila / Q000235:genetics; D004546:Elapid Venoms / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D051379:Mice; D015221:Potassium Channels / Q000378:metabolism*; D051381:Rats; D012836:Silver Proteins; D013045:Species Specificity; D013194:Staining and Labeling
D000906:Antibodies; D002352:Carrier Proteins; D004546:Elapid Venoms; D015221:Potassium Channels; D012836:Silver Proteins; C020273:dendrotoxin