Preparation and characterization of polyclonal antibodies specific for the 69 and 100 k-dalton forms of human 2-5A synthetase.
Recently, the existence of 40-, 46-, 69- and 100- kDa forms of 2',5'-oligoadenylate (2-5A) synthetase have been established in interferon-treated human cells. Using monoclonal antibodies specific for 69- and 100- kDa forms of 2-5A synthetase, we purified these proteins by immunoaffinity chromatography and raised murine polyclonal antibodies. All immunized mice developed antibodies (anti-69 or anti-100 kDa form) which were characterized by their capacity to immunoprecipitate [35S] cysteine labeled proteins from interferon-treated cells or identify these proteins by electrophoretic transfer immunoblot analysis of extracts from control and interferon-treated cells. The 69 and 100 kDa 2-5A synthetases were induced in different types of human cells, such as Daudi, BJAB, HeLa and differentiated HL-60 cells. These enzymes were not detectable nor induced in MRC5 and undifferentiated HL-60 cells.
Marié
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Institut Pasteur, Unité d'Oncologie Virale, Paris, France.
Galabru
J
J
Svab
J
J
Hovanessian
A G
AG
D015088:2',5'-Oligoadenylate Synthetase / Q000008:administration & dosage / Q000096:biosynthesis / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D001202:Ascitic Fluid / Q000032:analysis; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D051379:Mice; D008970:Molecular Weight; D014407:Tumor Cells, Cultured / Q000201:enzymology
D000906:Antibodies; D000939:Epitopes; D015088:2',5'-Oligoadenylate Synthetase