Protein stabilization by blocking the native unfolding nucleus.
Studies on the thermal inactivation of immobilized enzymes result in a novel conception of protein stabilization. The native protein molecule is suggested to be characterized by a specific structural region where the unfolding process starts. Accordingly, enzyme stabilization by immobilization is the result of blocking this unfolding nucleus.
Schellenberger
A
A
Department of Biotechnology, Martin-Luther University, Halle, GDR.
Ulbrich
R
R
D000681:Amylases / Q000378:metabolism*; D002918:Chymotrypsin / Q000378:metabolism*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006358:Hot Temperature; D011489:Protein Denaturation; D013816:Thermodynamics; D014357:Trypsin / Q000378:metabolism*
D004800:Enzymes, Immobilized; D000681:Amylases; D002918:Chymotrypsin; D014357:Trypsin