Cytotoxic T lymphocyte recognition of secreted HLA class I molecules.
The cytolytic responses of DBA/2 mice against syngeneic transfected P815 mastocytoma cells expressing either membrane-associated (HLA-Cw3) or -secreted hybrid (HLA-Cw3 x H-2 Q10b) molecules were compared. In spite of the absence of serologically detectable hybrid molecules on their plasma membrane, cells secreting these molecules elicited a CTL response similar to that of cells expressing the membrane associated HLA-Cw3 molecules, in terms of both MHC-restriction and peptide specificity. Together with the observation that syngeneic mice were capable of rejecting the injected secreting cells, these results imply that secreted HLA class I molecules can function as minor histocompatibility Ag and suggest that processing of both the membrane-bound and the -secreted forms of a protein may follow common or overlapping pathways.
Kahn-Perles
B
B
Centre d'Immunologie de Marseille-Luminy, France.
Barra
C
C
Jehan
J
J
Fourel
D
D
Barad
M
M
Maryanski
J L
JL
Lemonnier
F A
FA
D000818:Animals; D002460:Cell Line; D002999:Clone Cells / Q000276:immunology / Q000637:transplantation; D000939:Epitopes / Q000235:genetics; D005813:Genes, Synthetic; D006680:HLA Antigens / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D015236:HLA-C Antigens / Q000235:genetics; D006801:Humans; D008297:Male; D012515:Mast-Cell Sarcoma / Q000378:metabolism; D051379:Mice; D008811:Mice, Inbred DBA; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000637:transplantation; D014162:Transfection
D000939:Epitopes; D006680:HLA Antigens; D015236:HLA-C Antigens; C038653:HLA-C*03 antigen