The mycobacterial GroEL stress protein: a common target of T-cell recognition in infection and autoimmunity.
The 65 kD protein of mycobacteria is an immunodominant antigen for both T and B lymphocytes. Sequence analysis has revealed that this protein belongs to the highly conserved family of stress proteins, related to the GroEL gene product of E. coli, that are present in all cells from bacteria to man. We demonstrate here that human T cells from healthy individuals and disease sites are able to recognize determinants within the 65 kD protein that are either specific for M. tuberculosis or are conserved between GroEL of mycobacterial, E. coli or human origin. The induction of T cells that recognize with cross-reactive sequences of GroEL may provide an explanation for the autoimmune phenomena often associated with infection by microbial pathogens. However, both the magnitude and the biological significance of this component of the T-cell repertoire reactive with self stress proteins will be influenced by local environmental factors as well as the MHC haplotype of the individual.
Lamb
J R
JR
MRC Tuberculosis and Related Infections Unit, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Bal
V
V
Rothbard
J B
JB
Mehlert
A
A
Mendez-Samperio
P
P
Young
D B
DB
D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001327:Autoimmune Diseases / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D018834:Chaperonin 60; D003429:Cross Reactions; D000939:Epitopes; D004926:Escherichia coli / Q000276:immunology; D006360:Heat-Shock Proteins / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007239:Infections / Q000276:immunology; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology*
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D018834:Chaperonin 60; D000939:Epitopes; D006360:Heat-Shock Proteins; C052242:alpha antigens, Mycobacterium