Comparative studies of type X collagen expression in normal and rachitic chicken epiphyseal cartilage.
The levels of type X collagen in mineralizing normal chicken epiphyses and nonmineralizing rachitic chicken tibial epiphyses were measured and compared. Qualitative immunoperoxidase studies with anti-chick type X collagen monoclonal antibodies on sections from normal and rachitic cartilage demonstrated that the type X collagen levels in rachitic growth plates are reduced. Northern hybridization of mRNA and biosynthetic studies have confirmed that type X collagen synthesis in rickets is also decreased. In hypocalcemic rickets, the level of type X collagen mRNA is reduced by 80% whereas the level of type X collagen mRNA is only reduced by 50% in normocalcemic rickets. These observations provide additional evidence that type X collagen is involved in the process of cartilage mineralization and also suggest that the partial recovery of type X collagen synthesis in normocalcemic rickets may be related to the elevated plasma concentration of calcium. Calcium concentration may therefore play an important role in the control of type X collagen synthesis.
Kwan
A P
AP
Department of Biochemistry and Molecular Biology, University of Manchester, United Kingdom.
Dickson
I R
IR
Freemont
A J
AJ
Grant
M E
ME
D000818:Animals; D000911:Antibodies, Monoclonal; D015152:Blotting, Northern; D002645:Chickens; D003094:Collagen / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression; D006132:Growth Plate / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D007124:Immunoenzyme Techniques; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012016:Reference Values; D012279:Rickets / Q000378:metabolism* / Q000473:pathology
D000911:Antibodies, Monoclonal; D012313:RNA; D003094:Collagen