A simplified method of tissue processing for immunostaining with good preservation of antigens and morphology.
A simple and rapid method of tissue processing has been developed for immunostaining. Human and murine tissues were fixed in a PVA solution, diluted in a special buffer and embedded in paraffin or stored in a stock solution before preparing frozen sections. By indirect immunofluorescence, several antigens (collagen isotypes, laminin and fibronectin) were better demonstrated in the samples processed by the present method than with frozen or deparaffinized sections. In addition, this method allows a histological preservation quite identical to that seen in classical histology.
Bourges
M
M
Esterre
P
P
Guerret
S
S
Grimaud
J A
JA
D000818:Animals; D000941:Antigens / Q000032:analysis; D005109:Extracellular Matrix / Q000276:immunology; D006652:Histological Techniques; D006801:Humans; D007158:Immunologic Techniques; D011142:Polyvinyl Alcohol; D013194:Staining and Labeling / Q000379:methods*
D000941:Antigens; D011142:Polyvinyl Alcohol