title: In situ staining of bromodeoxyuridine positive cells in normal and neoplastic colony-forming units grown on plasma clots.
abstract: Bromodeoxyuridine, an analogue of thymidine, can be detected by means of monoclonal antibodies and utilized as a marker of the S-phase of the cell cycle. In this paper a method for the detection of the labeling index of normal and neoplastic colony-forming units (CFU) growing in plasma clot semisolid medium is described and preliminary results on the cell cycle of 7th and 14th CFU granulocyte-macrophage are discussed.
authors: Istituto di Ematologia Seràgnoli, Università di Bologna, Italy.
authors: P L
authors: PL
authors: Tazzari
authors: M
authors: M
authors: Gobbi
authors: C
authors: C
authors: Tassi
authors: R M
authors: RM
authors: Lemoli
authors: A
authors: A
authors: Dinota
authors: G
authors: G
authors: Visani
authors: S
authors: S
authors: Tura
mesh_terms: D001777:Blood Coagulation; D001973:Bromodeoxyuridine; D002449:Cell Aggregation; D002455:Cell Division; D002460:Cell Line; D002999:Clone Cells; D003114:Colony-Forming Units Assay; D006412:Hematopoietic Stem Cells / Q000032:analysis* / Q000473:pathology; D006801:Humans; D014411:Neoplastic Stem Cells / Q000032:analysis* / Q000473:pathology; D010949:Plasma; D013194:Staining and Labeling / Q000379:methods
chemical_list: D001973:Bromodeoxyuridine