Identification of immunogenic regions of the major coat protein of human papillomavirus type 16 that contain type-restricted epitopes.
We have identified regions of the major capsid protein, L1, of the human papillomavirus (HPV) type 16 (HPV-16 L1), that are recognized by five monoclonal antibodies (MAbs) raised to a bacterial fusion protein containing residues 172 to 375 of HPV-16 L1. All five MAbs recognized HPV-16-infected tissue sections by immunohistochemistry, but not sections infected with HPV-1a (cutaneous warts), HPV-6b or -11 (genital warts). MAbs 3D1, 5A4 and 1D6 also recognized HPV-2-infected sections (cutaneous warts); MAb 8C4 recognized only sections containing HPV-16. Four MAbs (8C4, 3D1, 1D6 and 5A4) recognized a synthetic peptide corresponding to residues 269 to 284 of HPV-16 L1; within this region a minimum antibody binding site was identified, a tripeptide 276 to 278. However the complete epitope appears to extend beyond these residues and beyond HPV-16 L1 (269 to 284). The fifth MAb, 1C6, recognized bacterial fusion proteins containing HPV-6b L1, -16 L1 or -18 L1 using immunoblots, yet appeared HPV-16-specific when tested on infected tissue sections. This MAb recognized five amino acids within a different region of HPV-16 L1 (residues 299 to 313).
Cason
J
J
Richard Dimbleby Laboratory of Cancer Virology, St Thomas' Campus, London, U.K.
Patel
D
D
Naylor
J
J
Lunney
D
D
Shepherd
P S
PS
Best
J M
JM
McCance
D J
DJ
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology; D000915:Antibody Affinity; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D002213:Capsid / Q000235:genetics / Q000276:immunology*; D000939:Epitopes; D007132:Immunoglobulin Isotypes / Q000276:immunology; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics / Q000276:immunology*; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012995:Solubility; D013045:Species Specificity; D013329:Structure-Activity Relationship
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D007132:Immunoglobulin Isotypes; D011993:Recombinant Fusion Proteins