Reconstitution of cardiac gap junction channeling activity into liposomes: a functional assay for gap junctions.
Cardiac gap junctions were reconstituted into liposomes. To determine if reconstitution resulted in membrane channel formation, we developed an assay for channel function that used a liposome-entrapped peroxidase to detect entry of a substrate into the liposome. The data demonstrate, for the first time, that reconstituted gap junctions from heart are capable of channel-forming activity in artificial membranes.
Claassen
D E
DE
Center for Basic Cancer Research, Kansas State University, Manhattan 66502.
Spooner
B S
BS
D000818:Animals; D002458:Cell Fractionation; D002645:Chickens; D006321:Heart / Q000502:physiology*; D007106:Immune Sera; D007365:Intercellular Junctions / Q000502:physiology* / Q000648:ultrastructure; D007473:Ion Channels / Q000502:physiology*; D007700:Kinetics; D008081:Liposomes; D013109:Sphingomyelins
D007106:Immune Sera; D007473:Ion Channels; D008081:Liposomes; D013109:Sphingomyelins