Antibodies to ras oncogene p21 proteins lack immunohistochemical specificity for neoplastic epithelium in human prostate tissue.
Increased expression of ras oncogene protein p21 has been claimed to be associated with neoplastic transformation in histochemical studies of prostate and other tissues. Therefore, we evaluated the expression of p21 in formaldehyde-fixed, paraffin-embedded prostatic tissue using a commercially available polyclonal antiserum prepared in sheep. We found no consistent difference in p21 expression between benign and malignant epithelium and no correlation between tumor grade and intensity of p21 staining. Our results do not support the contention that neoplastic transformation of human prostate is associated with a histochemically demonstrable increase in p21 expression.
Varma
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VA
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, GA.
Austin
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GE
O'Connell
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AC
D000906:Antibodies / Q000276:immunology; D000918:Antibody Specificity; D014408:Biomarkers, Tumor; D002471:Cell Transformation, Neoplastic / Q000276:immunology; D004848:Epithelium / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D011518:Proto-Oncogene Proteins / Q000276:immunology*; D016283:Proto-Oncogene Proteins p21(ras); D013194:Staining and Labeling
D000906:Antibodies; D014408:Biomarkers, Tumor; D011518:Proto-Oncogene Proteins; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)