Immunogold-silver staining by capillary action.
The authors have developed an improved method for immunogold-silver staining of paraffin sections. Using a manual capillary action staining system, they were able to simplify the technical aspects of the procedure, permitting rapid processing of large batches of slides with better reproducibility. Background staining was decreased by use of buffers containing a detergent. The use of a light-stable silver reagent permitted greater control of the enhancement stage. The method yielded a high degree of contrast with negligible nonspecific staining. Sensitivity was comparable to that obtained with conventional enzymatic immunostaining. However, the authors noted that trypsinization of sections was rendered unnecessary for those antigens for which such pretreatment was usually required, and the need for special fixatives could be eliminated. The method was also applicable to immunostaining of frozen sections. Immunogold-silver staining by capillary action deserves consideration as an alternative to existing immunohistochemical methods in diagnostic histopathology.
Kumar
R K
RK
School of Pathology, University of New South Wales, Kensington, Australia.
Braye
S G
SG
Crouch
R L
RL
D002197:Capillary Action; D005629:Frozen Sections; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry / Q000379:methods*; D007515:Islets of Langerhans / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism; D008221:Lymphoid Tissue / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D009130:Muscle, Smooth / Q000378:metabolism*; D009369:Neoplasms / Q000378:metabolism*; D010950:Plasma Cells / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods