Corneal endothelial cell loss following trephination.
The amount of endothelial cell loss that occurs to the donor cornea during the trephination process was evaluated in 40 porcine eyes. A vital staining technique (alizarin red S and acid violet 19) was used to quantify the extent of endothelial damage and removal. Two types of corneal punches (Weck and H&I0 were used for trephination. The average amount of endothelial loss from the peripheral cornea was 7.9%, accounting for only a 3% loss of endothelial cells over the total surface area of the corneal button. a comparison of the Weck and H&I corneal punches showed no significant difference (P greater than 0.1) by Student's test for both endothelial cell damage and removal.
Clinch
T E
TE
Cornea Service, Wills Eye Hospital, Philadelphia, Pennsylvania 19107.
Fung
K L
KL
Laibson
P L
PL
D000818:Animals; D002470:Cell Survival; D003315:Cornea / Q000473:pathology; D016039:Corneal Transplantation; D004727:Endothelium / Q000473:pathology / Q000601:surgery; D013194:Staining and Labeling; D013552:Swine