Kinetics of Mg2+-ATPase inhibition by benthiocarb and its recovery by oximes and L-cysteine in brain of albino rat (Wistar strain).
Effects in vitro of benthiocarb on rat brain Mg2+-ATPase were studied to elucidate the interaction of benthiocarb with Mg2+-ATPase. Based on IC50 values, it was evident that Mg2+-ATPase was sensitive to benthiocarb. Non-competitive inhibition with respect to activation by ATP was indicated by decreased maximal velocity (V) without change in Michaelis Menten constant (Km). It was also noted that pyridine-2-aldoxime (30 microM), pyridine-4-aldoxime (35 microM) and L-cysteine (90 microM) neutralized the inhibition of benthiocarb (8 microM).
Veera Babu
G R
GR
Department of Zoology, S. V. University, Tirupati, India.
Ramesh Reddy
G
G
Chetty
C S
CS
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D017301:Ca(2+) Mg(2+)-ATPase / Q000037:antagonists & inhibitors*; D003545:Cysteine / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D007306:Insecticides / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D010091:Oximes / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013859:Thiocarbamates / Q000494:pharmacology*
D007306:Insecticides; D010091:Oximes; D013859:Thiocarbamates; C050580:pyridine-4-aldoxime; D000255:Adenosine Triphosphate; C060977:pyridine-2-aldoxime; C037925:benthiocarb; D017301:Ca(2+) Mg(2+)-ATPase; D003545:Cysteine