Characterization of the CB antigen, a DNA-binding protein recognized by autoantibodies and which has a differential expression in lymphoid cells.
We have analyzed the characteristics of the CB Ag, a nuclear protein recognized by autoantibodies. Approximately 4% (12 out of 280) of the antinuclear-positive sera examined contained anti-CB antibodies. By immunofluorescence, these sera brightly stained the nuclei of most cells analyzed, including peripheral lymphocytes, but only dull or no staining was observed in thymocytes or B cells of the bursa of Fabricius. The CB Ag has been characterized as a DNA-binding protein, dissociable from DNA at 1.5 M NaCl, and with a Mr of 40,000 Da. Moreover, the ability of the extracted Ag to bind back to DNA has enabled us to design an ELISA system for its detection.
Juarez
C
C
Department of Immunology, Hospital de Sant Pau, Barcelona, Spain.
Agusti
M
M
Gelpi
C
C
Vila
J L
JL
Amengual
M J
MJ
Rodriguez
J L
JL
D000328:Adult; D000368:Aged; D000818:Animals; D000974:Antibodies, Antinuclear; D000937:Antigen-Antibody Reactions; D034961:Antigens, Nuclear; D001324:Autoantigens / Q000276:immunology* / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000276:immunology* / Q000302:isolation & purification; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008099:Liver / Q000032:analysis; D008214:Lymphocytes / Q000032:analysis*; D008297:Male; D008970:Molecular Weight; D051381:Rats; D013154:Spleen / Q000032:analysis; D013194:Staining and Labeling
D000974:Antibodies, Antinuclear; D034961:Antigens, Nuclear; D001324:Autoantigens; C057697:CB antigen, nuclear; D004268:DNA-Binding Proteins