[Preparative isolation of individual tRNA Phe by high performance liquid reversed-phase chromatography].
A method of rapid preparative isolation of Phe-tRNA(Phe) (E. coli) of almost 100% purity (1800 pmoles per 1 OE260) is developed. In includes three successive chromatographies of total tRNA on C18-modified Lichrosphere Si-1000 in acetonitrile gradients. Other individual tRNAs can be isolated by the same approach.
Bulychev
N V
NV
Graĭfer
D M
DM
Karpova
G G
GG
Lebedev
A V
AV
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004926:Escherichia coli / Q000032:analysis; D012329:RNA, Bacterial / Q000302:isolation & purification; D012345:RNA, Transfer, Amino Acid-Specific / Q000302:isolation & purification*; D012360:RNA, Transfer, Phe / Q000302:isolation & purification*
D012329:RNA, Bacterial; D012345:RNA, Transfer, Amino Acid-Specific; D012360:RNA, Transfer, Phe
Preparativnoe vydelenie individual'noĭ tRNK Phe vysokoéffektivnoĭ zhidkostnoĭ obrashchenno-fazovoĭ khromatografieĭ.