Adaptive color basis transformation. An aid in image segmentation.
Multispectral images of stained cells enable the use of color differences to segment and/or to discriminate between image components, such as cell types and cellular subcomponents. When the spectral characteristics of the image components do not change over the area of a slide or from slide to slide, one can create a constant weighted linear combination of spectral images to generate one-dimensional or two-dimensional images that have the desired contrast between the image components that must be discriminated. However, when the spectral characteristics are not constant, i.e., when they vary from image to image, a constant weighted linear combination cannot be employed; instead, an appropriate solution must be found for each selected image. This is usually a time-consuming, manual procedure that cannot be employed in a fully automated process of discriminating and segmenting stained cells. This paper describes an algorithm that uses principal components decomposition basis vectors to generate a nonstatic weighted linear combination of color images that can be used by an automated system. This algorithm relies on a semiconstant relationship between the areas (sizes) of the image components that are to be discriminated and/or segmented. The technique has been successfully applied as an aid in the segmentation of images of stained cervical smears; the images were acquired with a three-chip CCD camera that generates three broad-band color images.
MacAulay
C
C
British Columbia Cancer Research Centre, Vancouver, Canada.
Tezcan
H
H
Palcic
B
B
D000465:Algorithms; D002467:Cell Nucleus / Q000166:cytology / Q000473:pathology; D003116:Color / Q000592:standards*; D003593:Cytoplasm / Q000166:cytology / Q000473:pathology; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods; D014621:Vagina / Q000166:cytology / Q000473:pathology; D014626:Vaginal Smears