title: CD15 monoclonal antibodies react with a phosphotyrosine-containing protein on the surface of human neutrophils.
abstract: mAb are useful as probes in the study of the roles of cell-surface components in neutrophil function. Many mAb that bind to human neutrophils react with the oligosaccharide lacto-N-fucopentaose III (CD15 antibodies). These antibodies, as well as several other widely used mAb reactive with human neutrophils, were employed to detect phosphoproteins present on these cells. Immunoprecipitation and subsequent gel electrophoresis of proteins from neutrophils labeled with [gamma-32P]ATP revealed a 170 to 190-kDa phosphoprotein specifically reactive with CD15 antibodies. No phosphoproteins were immunoprecipitated by CD11 or CD18 mAb. Phosphoamino acid analysis of the 170- to 190-kDa protein showed that it contained predominantly phosphotyrosine and a low level of phosphoserine. Recently, it was shown that this phosphoprotein is one of the major substrates of ecto-protein kinase activity on human neutrophils. The roles for the 170- to 190-kDa phosphoprotein and the ecto-protein kinase in neutrophil function remain to be determined.
authors: Department of Medicine, University of Minnesota Medical School, Minneapolis 55455.
authors: K M
authors: KM
authors: Skubitz
authors: J R
authors: JR
authors: Mendiola
authors: M S
authors: MS
authors: Collett
mesh_terms: D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D006801:Humans; D016256:Lewis X Antigen / Q000276:immunology*; D008565:Membrane Proteins / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D008970:Molecular Weight; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism; D009844:Oligosaccharides / Q000276:immunology*; D010750:Phosphoproteins / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D010766:Phosphorylation; D019000:Phosphotyrosine; D011233:Precipitin Tests; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000911:Antibodies, Monoclonal; D016256:Lewis X Antigen; D008565:Membrane Proteins; D009844:Oligosaccharides; D010750:Phosphoproteins; D019000:Phosphotyrosine; D014443:Tyrosine