title: Depression of cytochrome P450-dependent drug biotransformation by poly(rI.rC) and aspirin.
abstract: The inhibition of hepatic microsomal cytochrome P450 and cytochrome b5 levels by poly(rI.rC) and aspirin in vitro was studied in male Swiss mice. Poly(rI.rC) (10 mg/kg) decreases cytochrome P450 to a level of 61% and cytochrome b5 to 31%, while the activity of aminopyrine demethylase was decreased to 66%. Aspirin (200 mg/kg) decreased the level of cytochrome P450 and cytochrome b5 to 45 and 23%, respectively. Further studies on the effect of poly(rI.rC) showed that this decrease in the levels of cytochromes was mainly due to alteration in the protein synthesis of these hemoproteins.
authors: Department of Biochemistry, Panjab University, Chandigarh.
authors: S K
authors: SK
authors: Jagota
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000633:Aminopyrine N-Demethylase / Q000378:metabolism; D000818:Animals; D001241:Aspirin / Q000494:pharmacology*; D001711:Biotransformation; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D003573:Cytochrome b Group / Q000378:metabolism; D015786:Cytochromes b5; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D011070:Poly I-C / Q000494:pharmacology*; D012313:RNA / Q000378:metabolism
chemical_list: D000596:Amino Acids; D003573:Cytochrome b Group; D004364:Pharmaceutical Preparations; D012313:RNA; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System; D000633:Aminopyrine N-Demethylase; D011070:Poly I-C; D001241:Aspirin