[The effects of etretinate and anticancer drugs on the cell line of a squamous cell carcinoma of human skin (HSC-1)].
The purpose of this study is to examine the effects on the HSC-1 cell cycle of etretinate and anticancer drugs (BLM, 5-FU, MMC, PSK), using flow cytometry, and those of combined treatment on cell counts in HSC-1 cell culture. In the DNA histogram, when HSC-1 cells in the stationary period were exposed to etretinate, the S phase cells increased as a proportion of the cell population, mainly because of accelerated development from the G1 + 0 phase into the S phase. Combine treatment with etretinate, BLM and 5-FU decreased the number of HSC-1 cells to a greater extent than combined treatment with BLM and 5-FU. These results suggest that the stimulation of the G1 + 0 phase by etretinate enhanced the cytotoxicity of the anticancer drugs, and that a combination of etretinate with these drugs was effective in treating malignant epithelial tumors.
Tokuhashi
K
K
D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D001761:Bleomycin / Q000008:administration & dosage; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002452:Cell Count / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D004357:Drug Synergism; D005050:Etretinate / Q000008:administration & dosage / Q000494:pharmacology*; D005472:Fluorouracil / Q000008:administration & dosage; D006801:Humans; D016685:Mitomycin; D008937:Mitomycins / Q000008:administration & dosage; D012878:Skin Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology
D000970:Antineoplastic Agents; D008937:Mitomycins; D001761:Bleomycin; D016685:Mitomycin; D005050:Etretinate; D005472:Fluorouracil