Expression and electrophysiological identification of the receptor for bombesin and gastrin-releasing peptide in Xenopus laevis oocytes injected with polyA+ RNA from rat brain.
The receptor for bombesin and the related peptide, gastrin-releasing peptide (GRP) has been induced in frog oocytes by injection of polyA+ RNA from rat brain. The primed oocytes responded to peptides of the bombesin family (GRP, neuromedin C of bombesin) by showing dose-dependent oscillations in membrane currents as recorded by the voltage-clamp method. The induced membrane changes were suppressed when oocytes were pretreated with a bombesin-receptor antagonist.
Meyerhof
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W
Institut für Zellbiochemie und klinische Neurobiologie, Universitäts-Krankenhaus Eppendorf, Hamburg, FRG.
Morley
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SD
Richter
D
D
D000818:Animals; D001839:Bombesin / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D002462:Cell Membrane / Q000502:physiology; D005260:Female; D019886:Gastrin-Releasing Peptide; D008564:Membrane Potentials; D008845:Microinjections; D009865:Oocytes / Q000502:physiology*; D010455:Peptides / Q000378:metabolism*; D011061:Poly A / Q000235:genetics*; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics*; D012333:RNA, Messenger; D051381:Rats; D018004:Receptors, Bombesin; D017981:Receptors, Neurotransmitter / Q000235:genetics* / Q000502:physiology; D014982:Xenopus laevis
D010455:Peptides; D012333:RNA, Messenger; D018004:Receptors, Bombesin; D017981:Receptors, Neurotransmitter; D011061:Poly A; D012313:RNA; D019886:Gastrin-Releasing Peptide; D001839:Bombesin