title: Elimination of undesirable immunoglobulin contaminants including aggregated IgG from gamma-globulin preparations by jackfruit lectin affinity chromatography.
abstract: Jackfruit lectin, jacalin, has been characterized to combine with IgA of only IgA1 subclass but not with IgA2 subclass and other immunoglobulins. However, we found in the present study that a lectin from another batch of jackfruit (Jacalin-H) showed a different nature in binding reaction. Jacalin-H combined with immunoglobulins of every class or subclass except monomer IgG. More interestingly, the Jacalin-H also combined with aggregated IgG. By means of the Jacalin-H affinity chromatography, we could efficiently eliminate aggregated IgG as well as immunoglobulins other than monomer IgG from commercial gamma-globulin preparations. Jacalin-H affinity chromatography is easy, inexpensive and efficient in the elimination of undesirable immunoglobulin contaminants in the commercial gamma-globulin preparations.
authors: Department of Pediatrics, Yamaguchi University School of Medicine, Japan.
authors: H
authors: H
authors: Kondoh
authors: K
authors: K
authors: Kobayashi
mesh_terms: D002846:Chromatography, Affinity; D006801:Humans; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000302:isolation & purification*; D007075:Immunoglobulin M / Q000302:isolation & purification; D007136:Immunoglobulins / Q000302:isolation & purification*; D037102:Lectins; D037121:Plant Lectins; D005719:gamma-Globulins / Q000302:isolation & purification*
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D037102:Lectins; D037121:Plant Lectins; D005719:gamma-Globulins; C043696:jacalin; C044411:polymeric IgG