Cloning of a short HLA-DQ beta locus-specific cDNA probe: typing for DQw specificities.
A short HLA-DQ beta locus-specific (141 bp) probe was cloned from the full-length pII-beta-1 cDNA. Pst 1-digested genomic DNA from homozygous typing cell lines (HTC) was hybridized with this short DQ beta locus-specific, pDQ beta 141, probe. Restriction fragment length polymorphism (RFLP) patterns generated with this DQ beta locus-specific probe were compared with those obtained with the full-length (627 bp) DQ beta, pII-beta-1, probe. The results demonstrate that the RFLP patterns with the pDQ beta 141 probe were very simple, and no crossreacting DR beta and DX beta bands were observed. DQw1, 2, 3 and 4 specificities could each be identified by a single RFLP.
Sood
S K
SK
Department of Pathology, McMaster University, Hamilton, Ontario, Canada.
McCusker
C T
CT
Singal
D P
DP
D015139:Blotting, Southern; D003001:Cloning, Molecular; D015342:DNA Probes; D015343:DNA Probes, HLA; D000939:Epitopes / Q000032:analysis; D006683:HLA-DQ Antigens; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length
D015342:DNA Probes; D015343:DNA Probes, HLA; D000939:Epitopes; D006683:HLA-DQ Antigens; C051568:HLA-DQ1 antigen