High level of expression in the prostate of a human glandular kallikrein mRNA related to prostate-specific antigen.
Using a synthetic oligonucleotide primer complementary to human prostate-specific antigen mRNA, we found that an additional sequence possibly similar to human glandular kallikrein-1 could be read by a primer-extension sequencing technique. We were able to confirm the identity of that additional sequence with another oligonucleotide primer complementary to a specific region of the human glandular kallikrein-1 mRNA sequence. Northern blot analysis with 2 oligonucleotide probes respectively specific for prostate-specific antigen and human glandular kallikrein-1 mRNAs showed that the length of both mRNAs was similar at 1.5 kb. The level of human glandular kallikrein-1 mRNA relative to that of prostate-specific antigen could be estimated as approx. 10-20%. This study constitutes the first evidence that the human glandular kallikrein-1 gene is expressed at a high level in a human tissue.
Chapdelaine
P
P
Hormonal Bioregulation Laboratory, Laval University Hospital Centre, Ste-Foy, Quebec, Canada.
Paradis
G
G
Tremblay
R R
RR
Dubé
J Y
JY
D000951:Antigens, Neoplasm / Q000235:genetics*; D001483:Base Sequence; D003720:Densitometry; D005786:Gene Expression Regulation; D006801:Humans; D007610:Kallikreins / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D011467:Prostate / Q000276:immunology* / Q000378:metabolism; D017430:Prostate-Specific Antigen; D011471:Prostatic Neoplasms / Q000032:analysis / Q000276:immunology; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics
D000951:Antigens, Neoplasm; D012333:RNA, Messenger; D007610:Kallikreins; D017430:Prostate-Specific Antigen