Mitochondrial creatine kinase in cancer patients.
We have developed a quantitative immunoassisted enzyme assay to screen for creatine kinase isoenzymes BB (CK-BB) and mitochondrial (CK-m) using commercial antibodies and reagents. Presence of CK-m activity was subsequently confirmed by electrophoretic separation of samples with elevated values. A prospective clinical trial was undertaken in 117 subjects: Normal (30), cirrhosis patients (30), myocardial infarction patients (30), and untreated oncology patients with metastatic malignancy (27). In 12 patients with malignancy CK-m activities were elevated; all had adenocarcinomas. No significant activity was detected in patients with other malignancies. CK-m positive tumour patients had a significantly higher mortality rate, and in two instances death was preceded by a sudden rise in CK-m activity. We suggest CK-m is a marker of adenocarcinoma and its presence in serum signifies increased mortality.
Pratt
R
R
Division of Chemical Pathology, Wellington Hospital, New Zealand.
Vallis
L M
LM
Lim
C W
CW
Chisnall
W N
WN
D000230:Adenocarcinoma / Q000201:enzymology*; D000293:Adolescent; D000328:Adult; D001943:Breast Neoplasms / Q000201:enzymology*; D003402:Creatine Kinase / Q000032:analysis*; D005260:Female; D005770:Gastrointestinal Neoplasms / Q000201:enzymology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007527:Isoenzymes; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000201:enzymology*; D013194:Staining and Labeling / Q000379:methods
D007527:Isoenzymes; D003402:Creatine Kinase