Multiple sclerosis patients have a high frequency of an HLA-DQ beta epitope defined by a human-human hybridoma antibody.
Epstein-Barr virus (EBV) transformed B-cells from patients with multiple sclerosis (MS) and healthy controls were analysed for reactivity with the HLA-DQ-specific human-human hybridoma Ab TrB12 (anti-DQw6 + DQw8 + DQw9) by indirect immunofluorescence (IIF). Positive results were obtained with 34 out of 35 MS patients (97.1%) and 79 out of 106 controls (74.5%) (p less than 0.005, RR = 11.6). Thus, DQ molecules that express the TrB12 epitope may contribute to the susceptibility to develop MS.
Kolstad
A
A
Institute of Medical Biology, University of Tromsø School of Medicine, Norway.
Hannestad
K
K
Vandvik
B
B
Vartdal
F
F
D000906:Antibodies / Q000276:immunology*; D002461:Cell Line, Transformed; D000939:Epitopes / Q000276:immunology*; D006683:HLA-DQ Antigens / Q000276:immunology*; D004854:Herpesvirus 4, Human; D006801:Humans; D006825:Hybridomas / Q000276:immunology*; D009103:Multiple Sclerosis / Q000209:etiology / Q000276:immunology*; D009841:Oligonucleotides / Q000276:immunology
D000906:Antibodies; D000939:Epitopes; D006683:HLA-DQ Antigens; D009841:Oligonucleotides