title: [Restoration, by glycine betaine, of growth and enzyme activities of Escherichia coli Lac-mutants].
abstract: Lac- mutants of Escherichia coli which presented a growth triggered by adding glycine betaine to the medium were isolated and characterized. Glycine betaine restores beta-galactosidase (strain AM 12) and lactose permease (strain AT42) activities. It is suggested that the right and active conformation of these enzymes, lost during mutagenesis, is restored, in vivo, in presence of this betaine.
authors: Laboratoire de Microbiologie et Physiologie des Symbioses, C.N.R.S. U.A. n. 256, Université de Rennes-I.
authors: T
authors: T
authors: Bernard
authors: M
authors: M
authors: Ayache
authors: D
authors: D
authors: Le Rudulier
mesh_terms: D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D005998:Glycine / Q000031:analogs & derivatives*; D007785:Lactose / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000378:metabolism; D009154:Mutation; D012521:Sarcosine / Q000494:pharmacology*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D026901:Membrane Transport Proteins; D001616:beta-Galactosidase; D007785:Lactose; D005998:Glycine; D012521:Sarcosine
vernacular_title: Restauration, par la glycine bétaïne, de la croissance et d'activités enzymatiques de mutants Lac- d'Escherichia coli.