Bleomycin-induced DNA repair synthesis in permeable human fibroblasts: mediation of long-patch and short-patch repair by distinct DNA polymerases.
Treatment of permeable human fibroblasts with bleomycin elicits DNA repair synthesis that is only partially sensitive to aphidicolin, an inhibitor of mammalian DNA polymerases alpha and delta. Inhibition of long-patch repair synthesis by omission of the three unlabeled deoxyribonucleoside triphosphates (dNTPs) selectively eliminates the aphidicolin-sensitive component. The majority of this residual aphidicolin-resistant repair synthesis is contained in ligated patches as revealed by resistance to exonuclease III. Determination of repair patch length by bromodeoxyuridine-induced density shift under conditions where essentially all of the repair synthesis is sensitive or resistant to aphidicolin yielded values of approximately 20 and 4 nucleotides per patch, respectively. On the basis of these data and the relative sensitivity of bleomycin-induced repair synthesis to N2-(p-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate (BuPdGTP), 2',3'-dideoxythymidine 5'-triphosphate (ddTTP), and N-ethylmaleimide (NEM), long-patch repair is attributed to DNA polymerase delta and short-patch repair to DNA polymerase beta.
DiGiuseppe
J A
JA
Department of Pediatrics, St. Louis Children's Hospital, Washington University School of Medicine, Missouri 63110.
Dresler
S L
SL
D016590:Aphidicolin; D001761:Bleomycin / Q000494:pharmacology*; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D004249:DNA Damage; D004256:DNA Polymerase I / Q000378:metabolism*; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors; D004258:DNA Polymerase III / Q000378:metabolism*; D004260:DNA Repair / Q000187:drug effects; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004224:Diterpenes / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005092:Exonucleases / Q000378:metabolism; D005347:Fibroblasts; D006801:Humans; D013997:Time Factors
D004224:Diterpenes; D001761:Bleomycin; D016590:Aphidicolin; D004247:DNA; D004256:DNA Polymerase I; D004257:DNA Polymerase II; D004258:DNA Polymerase III; D004259:DNA-Directed DNA Polymerase; D005092:Exonucleases; D001973:Bromodeoxyuridine