title: Stimulation of pituitary luteinizing hormone secretion by gonadotropin-releasing hormone is not coupled to beta-luteinizing hormone gene transcription.
abstract: The physiological stimulation and inhibition of release of several peptide and protein hormones appears to be associated with transcription of the respective peptide- and protein hormone-encoding genes. In the current studies we investigated whether this was also true for the rat pituitary LH system. Using an anterior pituitary primary tissue culture system, we have analyzed the effects of 10(-9) M GnRH on beta LH gene transcription using a transcription run-on assay, and on nuclear beta LH RNA levels using a highly sensitive solution hybridization-S1-nuclease protection assay. GnRH-stimulated release of LH does not appear to be coupled to a significant change in the rate of beta LH gene transcription, but is associated with both a 2- to 3-fold increase in levels of the beta LH primary transcript and processing intermediates and a rapid decrease in the levels of fully processed beta LH mRNA in the nucleus. No significant change in beta LH cytoplasmic mRNA levels, however, was associated with GnRH-stimulated release of LH, in vitro. Our findings suggest that unlike several other peptide and protein hormone systems, stimulated release of beta LH by GnRH in vitro is not associated with an increase in beta LH gene transcription or cytoplasmic mRNA levels.
authors: Department of Biochemistry and Biophysics, Columbia University College of Physicians and Surgeons, New York, New York 10032.
authors: S R
authors: SR
authors: Salton
authors: M
authors: M
authors: Blum
authors: J A
authors: JA
authors: Jonassen
authors: R N
authors: RN
authors: Clayton
authors: J L
authors: JL
authors: Roberts
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D007986:Luteinizing Hormone / Q000235:genetics / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000378:metabolism; D010906:Pituitary Hormone-Releasing Hormones / Q000494:pharmacology*; D012313:RNA / Q000378:metabolism; D051381:Rats; D014158:Transcription, Genetic
chemical_list: D010906:Pituitary Hormone-Releasing Hormones; D012313:RNA; D007986:Luteinizing Hormone