Evaluation of a new monoclonal radioimmunoassay system for carcinoembryonic antigen.
A newly established monoclonal radioimmunoassay system (kit II) for carcinoembryonic antigen (CEA) was evaluated in comparison to a conventional polyclonal system (kit I). Among 619 unselected serum samples, approximately 15% were CEA positive with kit I, whereas 27% were positive with kit II; consequently, approximately 12% showed dissociated CEA values to be positive only with kit II. More than 75% of patients with the dissociated values were found to have a malignant disease. Positive rates in normal individuals (266 samples) were less than 5% with both the kit I and kit II and, in those with benign disease were 11.9% with kit II, except for liver cirrhosis in which a quite high positive rate (58%) was obtained. In contrast, a definitely increased positive rate in malignant disease (381 samples) was obtained with kit II (61.4%) in comparison to that with kit I (42.3%). The assessment of technical qualities, such as reproducibility, recovery and dilution tests, demonstrated the excellent performance of kit II.
Matsuoka
Y
Y
Department of Biochemistry, School of Medicine, Fukuoka University.
Kuroki
M
M
Okamoto
N
N
Ikeda
S
S
Hara
Y
Y
Minamizawa
T
T
Tachibana
S
S
Ogawa
H
H
Tabata
N
N
D000328:Adult; D000906:Antibodies; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor / Q000032:analysis; D002272:Carcinoembryonic Antigen / Q000032:analysis*; D004194:Disease; D000939:Epitopes / Q000032:analysis; D005069:Evaluation Studies as Topic; D006801:Humans; D008875:Middle Aged; D009369:Neoplasms / Q000097:blood; D011863:Radioimmunoassay / Q000379:methods; D011933:Reagent Kits, Diagnostic
D000906:Antibodies; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D000939:Epitopes; D011933:Reagent Kits, Diagnostic