Estrogen receptor analyses in breast cancer: comparison of monoclonal immunohistochemical and biochemical methods.
The estrogen receptor content in 100 female breast carcinomas was determined using the biochemical dextran-coated charcoal (DCC) method and the monoclonal estrogen receptor immunocytochemical assay (ER-ICA). Great care was taken to make procedures optimal in order to minimize preanalytical errors. A statistically highly significant correlation was found between the results of the two methods, which were qualitatively in accordance in 91 cases, while nine gave a negative ER-ICA but a weak positive DCC result. The quantitative correlations were also statistically significant but varied considerably in individual cases. Under optimal conditions, ER-ICA provides results similar to the DCC method, but it is still uncertain if it can stand alone in routine diagnosis.
Søndergaard
G
G
Institute of Pathology, Aalborg Hospital, Denmark.
Pedersen
K O
KO
Paulsen
S M
SM
D000911:Antibodies, Monoclonal; D001943:Breast Neoplasms / Q000032:analysis*; D002606:Charcoal; D003911:Dextrans; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D011960:Receptors, Estrogen / Q000032:analysis*
D000911:Antibodies, Monoclonal; D003911:Dextrans; D011960:Receptors, Estrogen; D002606:Charcoal