Picrosirius red staining of dental structures.
Histologic sections of dog mandibles and teeth were stained with picrosirius red and Mayer's hematoxylin. Collagenous structures of the mandible stained brilliant red. Dentinal tubules, Sharpey's fibers and other structures not easily seen in sections stained with hematoxylin and eosin alone were seen clearly after this procedure. Under polarized light collagen fibers could be specifically identified and their orientation determined. Picrosirius red-hematoxylin is recommended for examination of normal or pathologic dental specimens.
Roush
J K
JK
Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison 53706.
Breur
G J
GJ
Wilson
J W
JW
D000818:Animals; D001391:Azo Compounds; D003094:Collagen / Q000032:analysis; D004285:Dogs; D006652:Histological Techniques; D010851:Picrates; D013194:Staining and Labeling / Q000379:methods*; D014070:Tooth / Q000166:cytology*
D001391:Azo Compounds; D010851:Picrates; C009798:C.I. direct red 80; D003094:Collagen