Accurate quantitation of the low number of white cells in white cell-depleted blood components.
A simple method of accurate quantification of low concentrations of white cells (WBCs) in WBC-depleted blood components was developed by using propidium iodide (PI) to stain the nuclei of WBCs. The method was validated by correlating the PI-determined WBC concentrations with those determined with Coulter S-plus IV counter in units of packed red cells (PRBCs) or random-donor platelets (RDPs). The correlations were linear and had coefficients of 0.99. The sensitivity of PI staining permitted the detection of concentrations of WBCs as low as 1 cell per microL of RDPs or 11 cells per microL of PRBCs. Therefore, PI staining will be useful in investigating the role of transfused WBC-depleted blood components in the prevention of primary alloimmunization to HLA antigens, as well in evaluating various new procedures with high efficiency in depleting WBCs from blood components.
Kao
K J
KJ
Department of Pathology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville.
Scornik
J C
JC
D002469:Cell Separation; D004912:Erythrocytes / Q000166:cytology; D005374:Filtration; D006801:Humans; D007958:Leukocyte Count; D017713:Platelet Transfusion; D011419:Propidium; D013194:Staining and Labeling
D011419:Propidium