title: Total protein determined in human breast milk by use of coomassie brilliant blue and centrifugal analysis.
abstract: This simple method of centrifugal analysis for total protein in human breast milk is based on the change in the wavelength of the absorbance maximum of Coomassie Brilliant Blue G-250 when the dye is bound to protein. Within-run and between-day CVs were 3.8% and 4.8%, respectively. Compared with a micro-Kjeldahl method for determination of total nitrogen, the coefficient of correlation was 0.99.
authors: Department of Clinical Chemistry, Falun Central Hospital, Sweden.
authors: Y
authors: Y
authors: Bergqvist
authors: L
authors: L
authors: Karlsson
authors: L
authors: L
authors: Fohlin
mesh_terms: D000328:Adult; D001322:Autoanalysis; D002498:Centrifugation / Q000379:methods; D005260:Female; D006801:Humans; D008894:Milk Proteins / Q000032:analysis* / Q000592:standards; D008895:Milk, Human / Q000032:analysis*; D009584:Nitrogen / Q000032:analysis; D012394:Rosaniline Dyes; D013194:Staining and Labeling
chemical_list: D008894:Milk Proteins; D012394:Rosaniline Dyes; C004692:coomassie Brilliant Blue; D009584:Nitrogen