title: Lack of capillary recruitment in the brains of awake rats during hypercapnia.
abstract: The present study investigates the question of whether increases in CBF induced by hypercapnia in awake rats are accompanied by increases in the number of perfused capillaries. For the detection of perfused capillaries, gamma-globulin-coupled fluorescein isothiocyanate was injected intravenously. In 10 brain structures the density of perfused capillaries per square millimeter was determined from coronal sections using a highly sensitive fluorescent microscopical method that, in contrast to others, avoided air drying of the frozen brain sections. The results showed an inhomogeneous local distribution of the density of perfused capillaries during normo- and hypercapnia. The density of perfused capillaries was unchanged during hypercapnia compared with normocapnia, although blood flow was markedly increased. It is concluded that a capillary recruitment does not exist in the brain during the high-flow situation of hypercapnia.
authors: Department of Physiology, University of Bonn, F.R.G.
authors: U
authors: U
authors: Göbel
authors: B
authors: B
authors: Klein
authors: H
authors: H
authors: Schröck
authors: W
authors: W
authors: Kuschinsky
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity; D001921:Brain / Q000098:blood supply* / Q000503:physiopathology; D002196:Capillaries / Q000503:physiopathology; D003911:Dextrans / Q000008:administration & dosage; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives; D005452:Fluoresceins / Q000008:administration & dosage; D005916:Globulins / Q000008:administration & dosage; D006935:Hypercapnia / Q000503:physiopathology*; D007275:Injections, Intravenous; D008297:Male; D010477:Perfusion; D051381:Rats; D011919:Rats, Inbred Strains; D013861:Thiocyanates / Q000008:administration & dosage
chemical_list: D003911:Dextrans; D005452:Fluoresceins; D005916:Globulins; D013861:Thiocyanates; C015219:fluorescein isothiocyanate dextran; D016650:Fluorescein-5-isothiocyanate