[Introduction of modified hexose residues into O-specific polysaccharides of Salmonella of serotype E, B, C2 and C3 using synthetic nucleotide sugars].
A series of synthetic nucleotide sugars have been studied as monosaccharide donors in the biosynthetic assembly of the O-antigen repeating units of Salmonella sero-groups E, B, C2 and C3. UDP-Tal did not serve as a substrate of galactosyl phosphate transferase, and GDP-Tal did not substitute GDP-Man in mannosyltransferase reactions. dTDP-L-Man proved to be a substrate of rhamnosyl transferases; GDP-2dMan, GDP-3dMan, GDP-D-Rha and GDP-Glc served as substrates for mannosyltransferases. The modified tri- and tetrasaccharide derivatives can be converted into the modified O-specific polysaccharides after incubation with Salmonella membranes.
Druzhinina
T N
TN
Gogilashvili
L M
LM
Shibaev
V N
VN
D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology*; D002240:Carbohydrate Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D006601:Hexoses; D008070:Lipopolysaccharides / Q000138:chemical synthesis* / Q000276:immunology; D009702:Nucleoside Diphosphate Sugars / Q000138:chemical synthesis*; D019081:O Antigens; D012475:Salmonella / Q000276:immunology*
D000942:Antigens, Bacterial; D006601:Hexoses; D008070:Lipopolysaccharides; D009702:Nucleoside Diphosphate Sugars; D019081:O Antigens
Vvedenie modifitsirovannykh ostatkov geksoz v O-spetsificheskie polisakharidy salmonell serogrupp E, B, C2 i C3 s pomoshch'iu sinteticheskikh nukleotidsakharov.