T cell receptor gene segment usage in a panel of hen-egg white lysozyme specific, I-Ak-restricted T helper hybridomas.
We have studied the relationship between MHC-restricted, Ag-specific recognition and TCR structure in a panel of seven Th-hybridomas specific for the foreign protein Ag, hen egg-white lysozyme, and the I-Ak class II MHC molecule. The fine specificity of these Th cells had been determined previously by their reactivity to a panel of APC lines bearing mutant I-Ak molecules and to proteolytic fragments of HEL. TCR gene segment composition was determined by cDNA cloning and DNA sequencing. A heterogeneous, yet repetitive usage of gene segments was observed within the panel. The same V alpha C10-J alpha MD13 rearrangement was used in three of the hybridomas, two with identical Ag and MHC-restriction fine specificities. The prevalent usage of the V beta 14 gene segment and members of J beta 2 cluster was noted. Inasmuch as gene segment usage did not correlate with MHC-restriction or Ag fine specificity alone, these results favor an interactive Ag model of T-cell recognition, in which Ag and MHC are recognized as a bimolecular complex.
Johnson
N A
NA
Department of Cancer Biology, Harvard School of Public Health, Boston, MA 02115.
Carland
F
F
Allen
P M
PM
Glimcher
L H
LH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D015387:Egg Proteins, Dietary / Q000276:immunology*; D000939:Epitopes; D015329:Gene Rearrangement, T-Lymphocyte; D000949:Histocompatibility Antigens Class II / Q000276:immunology*; D006825:Hybridomas / Q000032:analysis*; D007133:Immunoglobulin Joining Region / Q000235:genetics / Q000302:isolation & purification; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000302:isolation & purification; D051379:Mice; D008808:Mice, Inbred CBA; D008969:Molecular Sequence Data; D009113:Muramidase / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000032:analysis / Q000235:genetics*; D006377:T-Lymphocytes, Helper-Inducer / Q000032:analysis*
D015387:Egg Proteins, Dietary; D000939:Epitopes; D000949:Histocompatibility Antigens Class II; D007133:Immunoglobulin Joining Region; D007135:Immunoglobulin Variable Region; D011948:Receptors, Antigen, T-Cell; D009113:Muramidase