Alterations in the structure of new and old forms of simian virus 40 large T antigen (T) defined by age-dependent epitope changes: new T is the same as ATPase-active T.
The reactivity of simian virus 40 large T antigen labeled under pulse-chase conditions towards 22 antibodies was measured. Changes in epitope reactivity occurred in several domains of T as it matured, defining major structural alterations that distinguished mature from new molecules. New T reacted best with the same antibodies that bind and inhibit ATPase-active T. These antibodies thus can distinguish new T as a distinct structural and functional form.
Tack
L C
LC
Molecular Biology and Virology Laboratory, Salk Institute, La Jolla, California 92138-9216.
Wright
J H
JH
Gurney
E G
EG
D000251:Adenosine Triphosphatases / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000952:Antigens, Polyomavirus Transforming / Q000276:immunology* / Q000378:metabolism / Q000648:ultrastructure; D002522:Chlorocebus aethiops; D004268:DNA-Binding Proteins / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure; D000939:Epitopes; D051379:Mice; D009363:Neoplasm Proteins / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D011499:Protein Processing, Post-Translational; D013539:Simian virus 40 / Q000276:immunology*; D014157:Transcription Factors / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure; D016159:Tumor Suppressor Protein p53
D000911:Antibodies, Monoclonal; D000952:Antigens, Polyomavirus Transforming; D004268:DNA-Binding Proteins; D000939:Epitopes; D009363:Neoplasm Proteins; D010750:Phosphoproteins; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53; D000251:Adenosine Triphosphatases