Activation of the beta 2-adrenergic receptor promotes growth and differentiation in thyroid cells.
We have introduced the beta 2-adrenergic receptor into the unnatural environment of a thyroid cell to demonstrate that the activation of this receptor initiates diverse cellular programs in different cell types. The thyroid-stimulating hormone (TSH) receptor and the beta 2-adrenergic receptor stimulate a common signaling pathway in distinct populations of cells. In this study, we demonstrate that the activation of the beta 2-adrenergic receptor, transfected into a thyroid epithelial cell, elicits a program of growth and differentiation normally observed with TSH. In thyroid cells expressing beta 2 receptors, the beta 2 agonist isoproterenol activates adenylate cyclase, induces the expression of a thyroid-specific iodide carrier system, and can substitute for TSH to promote growth. Thus, in thyroid cells expressing beta 2-adrenergic receptors, isoproterenol elicits the entire array of thyroid-specific functions normally activated by TSH.
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Howard Hughes Medical Institute, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
Axel
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Obici
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D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D004261:DNA Replication; D006801:Humans; D007454:Iodides / Q000378:metabolism; D007545:Isoproterenol / Q000494:pharmacology; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000235:genetics / Q000502:physiology*; D013961:Thyroid Gland / Q000166:cytology* / Q000378:metabolism; D013972:Thyrotropin / Q000494:pharmacology; D014162:Transfection
D007454:Iodides; D011943:Receptors, Adrenergic, beta; D005576:Colforsin; D013972:Thyrotropin; D000242:Cyclic AMP; D007545:Isoproterenol; D015056:1-Methyl-3-isobutylxanthine