In vitro release of histamine from murine mast cells by block co-polymers composed of polyoxyethylene and polyoxypropylene.
A series of block co-polymers composed of polyoxyethylene and polyoxypropylene were investigated for their ability to induce in vitro activation of mouse mast cells. We found that six of these co-polymers could cause histamine release from mouse mast cells in vitro. At low concentrations, the most efficacious co-polymer, T130R2, caused rapid and extensive concentration-dependent release of histamine from mouse mast cells. The release process was not cytotoxic; it required metabolic energy and was not accompanied by release of lactate dehydrogenase. Optimal release of histamine was dependent on both calcium and sodium ions in the extracellular medium. The degree of in vitro histamine release correlated with in vivo inflammation and in vitro ionophore activity. We believe that this represents the first report of the activation of mediator-containing cells by an ionophore selective for monovalent cations. These copolymers may therefore represent new reagents for investigations of cellular excitation.
Atkinson
T P
TP
Department of Pathology, Emory University School of Medicine, Atlanta, GA 30322.
Smith
T F
TF
Hunter
R L
RL
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D004734:Energy Metabolism / Q000187:drug effects; D005110:Extracellular Space / Q000187:drug effects / Q000378:metabolism; D005260:Female; D006636:Histamine Release / Q000187:drug effects*; D007476:Ionophores; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008407:Mast Cells / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D011060:Poloxalene / Q000031:analogs & derivatives / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011108:Polymers
D000276:Adjuvants, Immunologic; D007476:Ionophores; D011108:Polymers; D011092:Polyethylene Glycols; C035554:pluronic dibenzoyl ester; D011060:Poloxalene; D007770:L-Lactate Dehydrogenase; D002118:Calcium