Major histocompatibility complex class II (Ia-like) antigens on chicken embryo fibroblasts: effect of transformation by Rous sarcoma virus.
In this study, three monomorphic monoclonal antibodies to chicken MHC class II molecules (B-L) were tested for reactivity in normal and RSV-transformed embryo fibroblasts. The immunocytochemical staining, the cell-bound ELISA assay, and the immunoprecipitation analysis showed that all three antibodies reacted with the B-L (Ia-like) molecules on normal cells of different genotypes. Conversely, the expression of these antigens was not detected in fibroblasts cultured from feather follicles of adult birds. The level of expression of B-L molecules as well as the class II specific RNA increased consistently after transformation of the cells by the SR-RSV and infection with the avian leukosis virus RAV-1. Analysis of genomic DNA by the Southern blot technique, performed after digestion with several restriction endonucleases, showed that the restriction pattern of B-L genes was not altered in cells transformed by Rous sarcoma virus.
Gogusev
J
J
Laboratoire de Médecine expérimentale, Collège de France, Paris.
Suskind
G
G
Teutsch
B
B
Mongiat
F
F
Philippe
J
J
Haguenau
F
F
Hlozánek
I
I
D000818:Animals; D001354:Avian Leukosis Virus; D001358:Avian Sarcoma Viruses; D002472:Cell Transformation, Viral; D002642:Chick Embryo; D004247:DNA / Q000032:analysis; D004262:DNA Restriction Enzymes; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000276:immunology*; D015870:Gene Expression; D000949:Histocompatibility Antigens Class II / Q000032:analysis* / Q000235:genetics; D007150:Immunohistochemistry; D007163:Immunosorbent Techniques; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000096:biosynthesis
D000949:Histocompatibility Antigens Class II; D012313:RNA; D004247:DNA; D004262:DNA Restriction Enzymes