Expression of the embryonal neural cell adhesion molecule N-CAM in lung carcinoma. Diagnostic usefulness of monoclonal antibody 735 for the distinction between small cell lung cancer and non-small cell lung cancer.
Paraffin sections of 19 surgically resected small cell lung carcinomas (SCLC), 33 non-small cell lung carcinomas (NSCLC) of various types, and four bronchial carcinoids were immunostained with monoclonal antibodies (MoAbs) 735 and anti-Leu 7, both recognizing some sugar epitopes present on the neural cell adhesion molecule N-CAM. With MoAb 735, all SCLC were stained focally or diffusely, and one carcinoid was stained focally. Only three of the 33 NSCLC were faintly and focally positive with MoAb 735; these three tumours showed relatively small tumour cells and small, oval nuclei. Anti-Leu 7 stained all the carcinoids, only eight SCLC, sometimes focally, and eight NSCLC. MoAb 735 was thus superior to anti-Leu 7 in distinguishing between SCLC and NSCLE. Since MoAb 735 stained all SCLC strongly and is applicable on paraffin sections, it provides a well-needed addition to the immunomarkers used in the diagnostic distinction of SCLC and NSCLC.
Kibbelaar
R E
RE
Department of Pathology, The Netherlands Cancer Institute, Amsterdam.
Moolenaar
C E
CE
Michalides
R J
RJ
Bitter-Suermann
D
D
Addis
B J
BJ
Mooi
W J
WJ
D000911:Antibodies, Monoclonal; D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis / Q000473:pathology*; D018288:Carcinoma, Small Cell / Q000175:diagnosis / Q000473:pathology*; D002448:Cell Adhesion; D000939:Epitopes / Q000032:analysis; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis / Q000473:pathology*
D000911:Antibodies, Monoclonal; D000939:Epitopes