Opsin- and S-antigen-like immunoreactions in photoreceptors of the tree shrew retina.
In the tree shrew retina individual rod and cone photoreceptors can be readily identified and quantified because their perikarya are arranged in a single layer. This retina is therefore an ideal system for testing the specificity of photoreceptor-directed antibodies. Here we describe the staining properties of polyclonal antibodies against (rhod)opsin and retinal S-antigen in the tree shrew retina. The (rhod)opsin antibody exclusively and completely labelled the rod population. The antibody against S-antigen also labelled all rods and, in addition, a regularly arrayed subpopulation of cones, which we argue to be the blue-sensitive cones. In the context of our findings, the labelling of pinealocytes with these antibodies is discussed.
Müller
B
B
Max-Planck-Institut für Hirnforschung, Frankfurt, Federal Republic of Germany.
Peichl
L
L
De Grip
W J
WJ
Gery
I
I
Korf
H W
HW
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000941:Antigens / Q000276:immunology*; D019393:Arrestin; D005136:Eye Proteins / Q000276:immunology*; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D010786:Photoreceptor Cells / Q000276:immunology*; D012160:Retina / Q000166:cytology / Q000276:immunology* / Q000648:ultrastructure; D012243:Rhodopsin / Q000276:immunology; D017299:Rod Opsins; D013194:Staining and Labeling; D014418:Tupaiidae
D000906:Antibodies; D000941:Antigens; D019393:Arrestin; D005136:Eye Proteins; D017299:Rod Opsins; D012243:Rhodopsin