title: Antagonists of luteinizing hormone releasing hormone bind to rat mast cells and induce histamine release.
abstract: It was reported previously that administration of certain synthetic antagonists of LHRH to rats produced allergy-like symptoms that were attributed to their histamine releasing action. In the present study the interaction of LHRH analogs with rat peritoneal mast cells was investigated in vitro. Potent antagonists of LHRH showed strong in vitro histamine releasing activity from rat peritoneal mast cells. Membrane preparations of rat pituitary glands showed specific binding of radioiodinated LHRH antagonist as well as LHRH agonist. However, rat peritoneal mast cells and membrane preparations from those cells bound antagonist but not the agonist. Furthermore, the LHRH antagonist did not bind to membranes prepared from tissues such as prostate, liver, kidney, and brain. Competitive displacement curves of the [125I]-antagonist with different LHRH analogs showed that the ability of the analogs to compete for binding sites on mast cells was related to their histamine releasing activity. We conclude that histamine release from rat mast cells induced by LHRH analogs is mediated by specific binding of the active peptides to cell membranes. Furthermore, using rat mast cells, the binding assay in conjunction with histamine releasing assay may be utilized to predict the in vivo histamine releasing potential of new LHRH peptides which are of clinical importance.
authors: Center for Biomedical Research, Population Council, New York, N.Y. 10021.
authors: K
authors: K
authors: Sundaram
authors: A
authors: A
authors: Didolkar
authors: R
authors: R
authors: Thau
authors: M
authors: M
authors: Chaudhuri
authors: F
authors: F
authors: Schmidt
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors* / Q000378:metabolism; D006636:Histamine Release; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D010902:Pituitary Gland / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D007987:Gonadotropin-Releasing Hormone