Epitope analysis: biotinylated short peptides as inhibitors of anti-peptide antibody.
The interaction of anti-melittin antisera with melittin-coated ELISA plates could be inhibited by biotinylated peptides of the C terminal epitope which is one of three defined antigenic sites on the hexacosapeptide melittin. Non-biotinylated short peptides and peptide derivatives were inactive. It is suggested that biotinylation of epitopic peptides enhances their inhibitory properties in a methodologically useful way.
Von Grünigen
R
R
University of Bern, Institute for Clinical Immunology, Inselspital, Switzerland.
Schneider
C H
CH
D000595:Amino Acid Sequence; D001514:Bee Venoms / Q000276:immunology*; D001667:Binding, Competitive; D001710:Biotin; D000939:Epitopes; D008555:Melitten / Q000276:immunology*; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology*; D013329:Structure-Activity Relationship
D001514:Bee Venoms; D000939:Epitopes; D010455:Peptides; D008555:Melitten; D001710:Biotin