[Detection of the human immunodeficiency virus in a lymphocyte culture from a patient from Central Africa with acquired immunodeficiency syndrome and Kaposi's sarcoma].
The results of HIV virus isolation from patient A. T., resident of Central Africa, are discussed, in whom Kaposi sarcoma was diagnosed clinically and histologically, and immunological examinations revealed the acquired immunodeficiency syndrome (AIDS). Cocultivation of the peripheral blood leukocytes of the patient with normal donor leukocytes yielded a leukocyte culture in which HIV virus expression was demonstrated. The HIV antigen was detected in the ultracentrifugate of the cultures by means of indirect enzyme-immunoassay using sera to the virus from the standard kits of Organon (Netherlands) and Abbot (USA). HIV antigen was also detected on the surface of lymphocytes in the culture by indirect immunofluorescence using the same sera. Besides, reverse transcriptase activity was demonstrated in ultracentrifugates of the culture fluid by exogenous reverse transcription test using poly(zA)-oligo(dT) matrix.
Zhdanov
V M
VM
Parfanovich
M I
MI
Iaroslavtseva
N G
NG
Kucherov
I I
II
Potekaev
N S
NS
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D000328:Adult; D000350:Africa, Central; D000914:Antibodies, Viral / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006678:HIV / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007124:Immunoenzyme Techniques; D008214:Lymphocytes / Q000382:microbiology*; D008297:Male; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D012514:Sarcoma, Kaposi / Q000382:microbiology*; D014461:Ultracentrifugation
D000914:Antibodies, Viral; D000956:Antigens, Viral; D012194:RNA-Directed DNA Polymerase
Obnaruzhenie virusa immunodefitstita cheloveka v kul'ture limfotsitov ot bol'nogo iz Tsentral'noĭ Afriki s sindromom priobretennogo immunodefitsita i sarkomoĭ Kaposhi.