Dolichos biflorus agglutinin (DBA) reveals a similar basal cell differentiation in normal and psoriatic epidermis.
Binding of N-acetyl galactosamine (GalNAc)-specific Dolichos biflorus agglutinin (DBA) conjugates to frozen sections of normal epidermis and of psoriatic uninvolved and lesional skin was studied in fluorescence microscopy. The DBA conjugates bound only to single basal cell layer in normal and uninvolved psoriatic epidermis from patients with different blood group status. In the lesional area of psoriatic skin a similar reaction with a single basal cell layer was revealed. Other lectin-conjugates applied, presenting also GalNAc specificity, reacted with most cell layers of normal and both uninvolved and lesional psoriatic epidermis and gave an attenuated reaction with the middle epidermal layers. The results show that the basal cell characteristics are confined only to the cells along the basal membrane also in psoriatic epidermis, although cells in three lowest layers may be able to proliferate.
Kariniemi
A L
AL
Department of Dermatology, Helsinki University Central Hospital, Finland.
Virtanen
I
I
D000017:ABO Blood-Group System / Q000502:physiology; D000116:Acetylgalactosamine / Q000378:metabolism; D001665:Binding Sites; D002454:Cell Differentiation / Q000502:physiology; D004817:Epidermis / Q000378:metabolism / Q000473:pathology*; D005629:Frozen Sections; D006801:Humans; D015603:Keratinocytes / Q000473:pathology; D037102:Lectins / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D037121:Plant Lectins; D011565:Psoriasis / Q000378:metabolism / Q000473:pathology*; D013194:Staining and Labeling
D000017:ABO Blood-Group System; D037102:Lectins; D037121:Plant Lectins; C036554:dolichos biflorus agglutinin; D000116:Acetylgalactosamine