Lectin reactivity of alpha-fetoprotein in a case of renal cell carcinoma.
The increased serum level of alpha-fetoprotein (AFP) in a case of renal cell carcinoma, a rare condition of AFP production by mesoderm-derived cells, was evaluated for its lectin reactivity by affinity electrophoresis, followed by the antibody-affinity transfer to nitrocellulose membranes for visualization of separated AFP bands. The AFP of this case was characterized by relative increases of concanavalin A-nonreactive AFP-C1 (60.4%), erythroagglutinating phytohemagglutinin-reactive AFP-P4 (37.8%) and AFP-P5 (46.3%) and Allomyrina dichotoma lectin-nonreactive AFP-A1s (66.7%), and by the total absence of lentil lectin-reactive components, AFP-L2 and AFP-L3. Thus, the lectin-reactive pattern of AFP markedly deviated not only from that of cord serum, but also from those of other malignancies and of fetal kidney cells in culture.
Taketa
K
K
Health Research Center, Kagawa University, Takamatsu, Japan.
Ichikawa
E
E
Sakuda
H
H
Iwamasa
T
T
Hayakawa
M
M
Taga
H
H
Hirai
H
H
D002292:Carcinoma, Renal Cell / Q000378:metabolism*; D003208:Concanavalin A / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010835:Phytohemagglutinins / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000378:metabolism*
D037102:Lectins; D010835:Phytohemagglutinins; D000509:alpha-Fetoproteins; D003208:Concanavalin A