Determination of kallikreinogen (KKN) in human plasma by activation with Hageman factor fragment (F XIIf).
On the basis of activation of KKN by F XIIf an assay for determination of KKN levels was developed. The esterolytic activity of the activation product PK was measured with BAEE as substrate. After maximal activation of normal human plasma the esterolytic activity amounts to 16.82 +/- 2.03 mukat/l plasma (n = 25). The within run precision and day to day precision characterized by the variation coefficient were 2.5 and 4.9, respectively.
Lehmann
B
B
Institute of Pathological Biochemistry, Medical Academy Carl Gustav Carus Dresden.
Taucher
M
M
Kühne
H
H
Scheuch
D W
DW
D001120:Arginine / Q000031:analogs & derivatives; D016264:Dextran Sulfate; D003911:Dextrans / Q000494:pharmacology; D005174:Factor XII / Q000494:pharmacology*; D015956:Factor XIIa; D006801:Humans; D007610:Kallikreins / Q000097:blood*; D008934:Mitogens / Q000494:pharmacology; D012697:Serine Endopeptidases / Q000494:pharmacology*
D003911:Dextrans; D008934:Mitogens; D005174:Factor XII; D016264:Dextran Sulfate; D001120:Arginine; C009727:benzoylarginine ethyl ester; D007610:Kallikreins; D012697:Serine Endopeptidases; D015956:Factor XIIa