title: Do monoclonal antibodies recognize linear sequential determinants?
abstract: A group of 19 anti-class II monoclonal antibodies produced in different laboratories were tested in ELISA for their ability to bind to a panel of synthetic peptides selected from HLA-DQ alpha and beta chains. No one of the antibodies tested was found to react with the synthetic fragments, thus confirming the common finding that MoAbs generally fail to recognize fragments of the native antigen. The possibility that this result might be partly due to the procedure used for screening hybridoma supernatants is discussed.
authors: Regina Elena Institute for Cancer Research, Rome, Italy.
authors: M
authors: M
authors: Camera
authors: E
authors: E
authors: Muratti
authors: M L
authors: ML
authors: Trinca
authors: A
authors: A
authors: Chersi
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D006683:HLA-DQ Antigens / Q000276:immunology*; D006801:Humans; D046911:Macromolecular Substances; D010446:Peptide Fragments / Q000276:immunology; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D006683:HLA-DQ Antigens; D046911:Macromolecular Substances; D010446:Peptide Fragments; D010455:Peptides