Analysis of potential expression of highly related members of the ribosomal protein L32 gene family.
The processed gene L32', a member of the mouse gene family for ribosomal protein L32, could encode a 135 amino acid protein nearly identical to L32. The 5'-flanking region of the gene contains CAAT and TATA sites at positions commonly found in expressed genes. The L32' gene lies within highly methylated, DNase I-insensitive chromatin of mouse L1210 cells. Although S1 nuclease digestion studies suggested that an L32' transcript might be produced, an oligonucleotide probe specific for L32' mRNA, and RNase digestion of a cRNA probe to L32', indicated fewer than 0.1 L32' transcripts/cell. These results demonstrate that extreme caution is required when measuring transcription from related genes.
Jacks
C M
CM
Department of Genetics and Cell Biology, University of Minnesota, St Paul 55108.
Biltz
R E
RE
Hackett
P B
PB
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005798:Genes, Bacterial; D007939:Leukemia L1210 / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D011061:Poly A / Q000235:genetics; D012313:RNA / Q000235:genetics; D012333:RNA, Messenger; D015183:Restriction Mapping; D012269:Ribosomal Proteins / Q000235:genetics*; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D012333:RNA, Messenger; D012269:Ribosomal Proteins; C041806:ribosomal protein L32; D011061:Poly A; D012313:RNA