Myelin basic protein-specific CD4+ cytolytic T-lymphocyte clones isolated from multiple sclerosis patients.
In the present report we describe the isolation and characterization of stable, long-term, human T-lymphocyte clones specific for myelin basic protein (MBP) from multiple sclerosis (MS) patients. Isolation of these clones appeared possible only by seeding peripheral blood mononuclear cells into a limiting dilution microculture system containing MBP, autologous irradiated cells and Interleukin-2 (IL-2), thereby minimizing effects of putative suppressor cell populations. All clones obtained were of the CD4+ phenotype. The majority was capable of MBP-specific cytolysis, tested with 51Chromium-labeled autologous Epstein-Barr virus (EBV)-transformed B-cells, coated with MBP, as targets. A few other clones had natural killer (NK) function. All clones produced Interleukin-2 (IL-2) upon adequate stimulation.
Weber
W E
WE
Department of Neurology, Academic Hospital Maastricht, University of Limburg, The Netherlands.
Buurman
W A
WA
D000945:Antigens, Differentiation, T-Lymphocyte; D002469:Cell Separation; D002999:Clone Cells / Q000276:immunology; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis; D009103:Multiple Sclerosis / Q000097:blood / Q000276:immunology*; D004676:Myelin Basic Protein / Q000276:immunology*; D010641:Phenotype; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000945:Antigens, Differentiation, T-Lymphocyte; D007376:Interleukin-2; D004676:Myelin Basic Protein