Determination of the RNA-content in samples of isolated chromatin by circular dichroism measurements.
A method is presented to determine the RNA content in isolated chromatin. An increased ellipticity at 260 nm-290 nm in the circular dichroism spectrum of chromatin is due to RNA released together with chromatin from the cell nuclei. Mixtures of chicken erythrocyte chromatin and pure 5s-RNA were used to reconstruct such spectra. From the obtained data an RNA content higher than 3-5% in samples of isolated chromatin can be determined.
Notbohm
H
H
Institut für Medizinische Molekularbiologie, Medizinische Universität zu Lübeck, FRG.
D000818:Animals; D002478:Cells, Cultured; D002645:Chickens; D002843:Chromatin / Q000032:analysis*; D002942:Circular Dichroism; D006367:HeLa Cells; D006801:Humans; D012313:RNA / Q000032:analysis*; D014407:Tumor Cells, Cultured
D002843:Chromatin; D012313:RNA