Detection of proteinase inhibitors in fractions after liquid chromatography.
A technique for rapid detection of proteinase inhibitors in fractions after liquid chromatography is described. Aliquots of the tested solutions are placed onto a thin layer of gelatin, crosslinked with glutardialdehyde in the presence of water soluble nigrosin, to form small drops. A proteinase solution is added to the above-mentioned drops and the plate is incubated at ambient temperature for approximately 15-25 min. After the gelatin layer is washed with water the inhibitor negative fractions are visualized as colorless zones on a blue background. With inhibitor positive fractions no change of gelatin layer occurs.
Safarík
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I
Laboratory of Analytical Chemistry, South Bohemian Biological Centre, Ceské Budĕjovice, Czechoslovakia.
D007611:Aprotinin / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D005782:Gels; D011480:Protease Inhibitors / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D005782:Gels; D011480:Protease Inhibitors; D007611:Aprotinin