Anatomy of a new B-cell-specific enhancer.
The major histocompatibility complex class II molecules, like the immunoglobulins, are prominent B-lymphocyte markers. Herein, we describe a B-cell-specific enhancer associated with the murine class II gene, Ek alpha. This enhancer has a complex anatomy that suggests interactions between remotely spaced elements. Of particular interest is the finding that two CCAAT boxes spaced one kilobase apart are important for enhancer activity. Somewhat surprisingly, the E alpha and immunoglobulin enhancers seem to show little resemblance.
Koch
W
W
Laboratoire de Génétique Moléculaire des Eucaryotes, Centre National de la Recherche Scientifique, Strasbourg, France.
Benoist
C
C
Mathis
D
D
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D005801:Genes, Homeobox; D005802:Genes, MHC Class II; D005822:Genetic Vectors; D007136:Immunoglobulins / Q000235:genetics; D051379:Mice; D009154:Mutation; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012313:RNA / Q000032:analysis; D015183:Restriction Mapping; D014162:Transfection
D004268:DNA-Binding Proteins; D007136:Immunoglobulins; D012313:RNA