The nucleotide sequence of satellite RNA in grapevine fanleaf virus, strain F13.
The nucleotide sequence of cDNA copies of grapevine fanleaf virus (strain F13) satellite RNA has been determined. The primary structure obtained was 1114 nucleotides in length, excluding the poly(A) tail, and contained only one long open reading frame encoding a 341 residue, highly hydrophilic polypeptide of Mr37275. The coding sequence was bordered by a leader of 14 nucleotides and a 3'-terminal non-coding region of 74 nucleotides. No homology has been found with small satellite RNAs associated with other nepoviruses. Two limited homologies of eight nucleotides have been detected between the satellite RNA in grapevine fanleaf virus and those in tomato black ring virus, and a consensus sequence U.G/UGAAAAU/AU/AU/A at the 5' end of nepovirus RNAs is reported. A less extended consensus exists in this region in comovirus and picornavirus RNA.
Fuchs
M
M
Institut de Biologie Moléculaire des Plantes du C.N.R.S., Laboratoire de Virologie, Strasbourg, France.
Pinck
M
M
Serghini
M A
MA
Ravelonandro
M
M
Walter
B
B
Pinck
L
L
D000595:Amino Acid Sequence; D001483:Base Sequence; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010942:Plant Viruses / Q000235:genetics*; D012313:RNA / Q000235:genetics*; D019244:RNA, Satellite; D012367:RNA, Viral / Q000235:genetics*; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000235:genetics
D019244:RNA, Satellite; D012367:RNA, Viral; D014764:Viral Proteins; D012313:RNA