Phosphoseryl-tRNA in Escherichia coli.
Two seryl-tRNAs, which were prepared from natural suppressor tRNA-rich fractions on a pattern of chromatography on Sephadex A-50, were phosphorylated by a tRNA kinase in Escherichia coli B. A part of phosphate on the tRNA was confirmed to be phosphoserine. Phosphoseryl-tRNA is universal in bacteria and vertebrates. Phosphoseryl-tRNA should be an intermediate from seryl-tRNA to selenocysteyl-tRNA.
Mizutani
T
T
Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Maruyama
N
N
Kurata
H
H
D002850:Chromatography, Gel; D004926:Escherichia coli / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010768:Phosphoserine / Q000032:analysis*; D012329:RNA, Bacterial / Q000032:analysis*; D012345:RNA, Transfer, Amino Acid-Specific / Q000032:analysis*; D012362:RNA, Transfer, Ser / Q000032:analysis*; D012694:Serine / Q000031:analogs & derivatives*
D012329:RNA, Bacterial; D012345:RNA, Transfer, Amino Acid-Specific; D012362:RNA, Transfer, Ser; D010768:Phosphoserine; D012694:Serine