Dextran sulfate as an inhibitor against the human immunodeficiency virus.
Dextran sulfate (DS) is a potent inhibitor of the growth of human immunodeficiency virus type 1 (HIV-1) in the H9 cell. Its minimal inhibitory concentration is about 1 microgram/ml. Its therapeutic index is greater than or equal to 200 which is higher than that of 38 for zidovudine. At the ID100 range, DS blocks the synthesis of HIV-1 antigens completely for at least 21 days; zidovudine at the subtoxic concentration of 3 micrograms/ml is incapable of achieving such a complete blockage. DS is still active when added to H9 cell cultures 4 hr after the addition of HIV-1. DS does not inactivate extracellular HIV-1 and is incapable of inducing interferons. It interferes partially with the infection of the H9 cells by the HIV-1. It inhibits the activity of HIV-1 reverse transcriptase. These activities may account, at least in part, for the inhibitory activity of dextran sulfate against the HIV-1. DS has a narrow antiviral spectrum; it is noninhibitory to the herpes simplex, vesicular stomatitis, polio, or adeno viruses. Dextran is not inhibitory to HIV-1. After sulfonation, the sulfonated dextran is highly inhibitory. Therefore, the sulfate group in the DS molecule appears to be essential for its anti-HIV-1 activity. The molecular weights of DS within the range 4000 to 12,000 do not appear to influence its anti-HIV potency.
Chang
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Department of Medical Microbiology and Immunology, University of California, Davis 95616.
Tabba
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He
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Smith
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D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology; D000998:Antiviral Agents / Q000633:toxicity*; D016264:Dextran Sulfate; D003911:Dextrans / Q000633:toxicity*; D006678:HIV / Q000187:drug effects* / Q000201:enzymology; D006801:Humans; D007369:Interferon Inducers / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D018894:Reverse Transcriptase Inhibitors; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects / Q000382:microbiology; D010948:Viral Plaque Assay; D014771:Virion / Q000187:drug effects; D015215:Zidovudine / Q000633:toxicity
D000998:Antiviral Agents; D003911:Dextrans; D007369:Interferon Inducers; D018894:Reverse Transcriptase Inhibitors; D015215:Zidovudine; D016264:Dextran Sulfate