title: Manganese(II) dynamics and distribution in glial cells cultured from chick cerebral cortex.
abstract: The kinetics of manganese(II) ion uptake and efflux have been investigated using tracer 54Mn(II) with glial cells cultured from chick cerebral cortex in chemically defined medium. The initial velocity of Mn(II) uptake versus [Mn(II)] exhibit saturation, with an apparent S0.5 approximately 18(+/- 3) microM. Both the rate and extent of Mn(II) uptake are inhibited by Ca(II), either added externally or preloaded into the glial cells. Preloading of glia with Mn(II) also inhibits the rate of external 54Mn(II) uptake. Zn(II) inhibits but Cu(II) activates Mn(II) uptake. Efflux of Mn(II) from preloaded cells occurs as a biphasic process, with rapid release of 30-40% of total cell Mn(II), then much slower release of the remainder. Permeabilization of cells with dextran sulfate also rapidly released ca. 30% of total cell Mn(II). High external Mn(II) enhanced both the rate and extent of Mn(II) efflux. CCCP, an uncoupler of oxidative phosphorylation, inhibited both Mn(II) uptake and efflux significantly, but addition of cyanide, ouabain, insulin, hydrocortisone, K+, or Nd(III) had no effect on either process. Taken together, these data suggest a model in which Mn(II) is brought across the plasma membrane by facilitated diffusion, binds to cytosolic protein sites, and is partitioned into the mitochondria by an active transport mechanism. The fact that the Mn(II) flux rates observed with cultured glia are much faster than those reported for overall uptake and efflux of brain Mn(II) in vivo suggests that the blood-brain barrier may play a significant role in determining these latter rates in whole animals.
authors: Department of Molecular & Cell Biology, Pennsylvania State University, University Park 16802.
authors: F C
authors: FC
authors: Wedler
authors: B W
authors: BW
authors: Ley
authors: A A
authors: AA
authors: Grippo
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D002642:Chick Embryo; D016264:Dextran Sulfate; D003911:Dextrans / Q000494:pharmacology; D004734:Energy Metabolism / Q000187:drug effects; D008345:Manganese / Q000493:pharmacokinetics*; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism*; D011190:Potassium Cyanide / Q000494:pharmacology
chemical_list: D003911:Dextrans; D008345:Manganese; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D000255:Adenosine Triphosphate; D016264:Dextran Sulfate; D011190:Potassium Cyanide