HLA-DR molecules on all cells are electrophoretically unique.
Previous SDS-PAGE studies of autologous clonal ALL and normal B cell lines indicated that HLA-DR molecules on leukemic cells have an extra beta chain band. We now show that these results are not an artifact of cell lines, EBV transformation, or cell growth in culture. Leukemic cells from four ALL patients were surface labeled with 125I and their HLA-DR molecules compared with those on autologous normal peripheral blood B cells. The electrophoretic patterns of HLA-DR molecules on these in vivo cell populations are identical to those on the corresponding cell lines. Moreover, EBV-transformation does not alter the electrophoretic appearance of HLA-DR molecules on normal tonsil B cells. Cell lines can now be used to study the chemical basis for these electrophoretic differences.
Pesando
J M
JM
Fred Hutchinson Cancer Research Center, Seattle, WA.
Hoffman
P
P
Stucki
M A
MA
D018941:Antigens, CD19; D000943:Antigens, Differentiation; D000944:Antigens, Differentiation, B-Lymphocyte; D000951:Antigens, Neoplasm; D001402:B-Lymphocytes / Q000032:analysis*; D002472:Cell Transformation, Viral; D004591:Electrophoresis, Polyacrylamide Gel; D006684:HLA-DR Antigens / Q000032:analysis*; D004854:Herpesvirus 4, Human; D006801:Humans; D015260:Neprilysin; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology*; D014407:Tumor Cells, Cultured
D018941:Antigens, CD19; D000943:Antigens, Differentiation; D000944:Antigens, Differentiation, B-Lymphocyte; D000951:Antigens, Neoplasm; D006684:HLA-DR Antigens; D015260:Neprilysin