Cyclic AMP as a negative regulator of DNA synthesis in FRSK cells, a fetal rat epidermal cell line.
In FRSK cells, a cell line derived from fetal rat epidermal cells, cyclic AMP-elevating agents forskolin (10 microM) and cholera toxin (10 ng/ml) increased cellular cyclic AMP content and suppressed [3H] thymidine incorporation. These effects of forskolin were enhanced by the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (0.1 mM). Dibutyryl cyclic AMP (1 mM), an analog of cyclic AMP, decreased not only basal but also both tumor promoter 12-O-tetradecanoylphorbol-13-acetate- and epidermal growth factor-stimulated [3H] thymidine incorporation. From these results, we suggest that cyclic AMP may be a negative regulatory factor of DNA synthesis in FRSK cells.
Yamanishi
K
K
Kishimoto
S
S
Yasuno
H
H
D015056:1-Methyl-3-isobutylxanthine; D000818:Animals; D002460:Cell Line; D002772:Cholera Toxin; D005576:Colforsin; D000242:Cyclic AMP / Q000032:analysis / Q000502:physiology; D004247:DNA / Q000096:biosynthesis*; D000078404:Epidermal Cells; D010726:Phosphodiesterase Inhibitors; D051381:Rats
D010726:Phosphodiesterase Inhibitors; D005576:Colforsin; D004247:DNA; D002772:Cholera Toxin; D000242:Cyclic AMP; D015056:1-Methyl-3-isobutylxanthine