[Polymerization of DNA fragments coding epitopes of the surface antigen of hepatitis B in Escherichia coli cells].
Synthetic oligonucleotides coding for the amino acid residues 135-151 (A, B) and 93-109 (C) of the hepatitis B surface antigen (HBsAg) have been polymerized. The obtained polymers (AC)n and (BCAC)n were inserted into the beginning of the lacZ gene under the control of the chloramphenicol promoter or the right promoter PR of bacteriophage lambda. Stability of the obtained plasmids was investigated during transformation, cell growth and gene expression.
Sergienko
O V
OV
Lunin
V G
VG
Tikhonenko
T I
TI
D000595:Amino Acid Sequence; D004247:DNA / Q000235:genetics*; D000939:Epitopes / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D005798:Genes, Bacterial; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D007763:Lac Operon; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes / Q000235:genetics; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid
D000939:Epitopes; D006514:Hepatitis B Surface Antigens; D009692:Nucleic Acid Heteroduplexes; D004247:DNA
Polimerizatsiia fragmentov DNK, kodiruiushchikh épitopy poverkhnostnogo antigena virusa gepatita B v kletkakh Escherichia coli.