Improved resolution of flow cytometric measurements of Hoechst- and chromomycin-A3-stained human chromosomes after addition of citrate and sulfite.
The resolution of bivariate flow karyotypes of human chromosomes stained with Hoechst 33258 and chromomycin A3 can be increased by adding sodium citrate and sodium sulfite to the chromosomes shortly before measurement. A flow karyotype of a patient with chronic myelocytic leukemia is shown to illustrate that the addition of these compounds allows high-resolution measurements to be made and evaluated reliably from clinical samples.
van den Engh
G
G
Lawrence Livermore National Laboratory-Biomedical Sciences Division, California 94550.
Trask
B
B
Lansdorp
P
P
Gray
J
J
D001562:Benzimidazoles; D006690:Bisbenzimidazole; D014128:Chromomycin A3; D002865:Chromomycins; D002951:Citrates; D019343:Citric Acid; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007621:Karyotyping; D007951:Leukemia, Myeloid / Q000235:genetics; D013194:Staining and Labeling; D013447:Sulfites; D014407:Tumor Cells, Cultured
D001562:Benzimidazoles; D002865:Chromomycins; D002951:Citrates; D013447:Sulfites; D019343:Citric Acid; D014128:Chromomycin A3; D006690:Bisbenzimidazole; C025026:sodium sulfite