A voltage-dependent calcium current in mouse Swiss 3T3 fibroblasts.
Patch-clamp experiments in the whole-cell mode have been performed in Swiss 3T3 mouse fibroblasts. Depolarizations from negative holding potential (Vh less than -60 mV) gave rise to a rapidly activating, fully inactivating, inward current of few tenths of nA in physiological saline at 35 degrees C. The current persisted when external Na+ was replaced by impermeant TMA+ and disappeared in 0 Ca2+, 1 mM EGTA. The current was reversible blocked by Co2+ and it was slightly reduced when external Ca2+ was substituted by Ba2+. Finally its reversal potential changed with Nernstian slope with increasing external Ca2+ concentrations. It is concluded that these cells possess a voltage-dependent Ca2+ channel.
Peres
A
A
Department of General Physiology and Biochemistry, University of Milan, Italy.
Zippel
R
R
Sturani
E
E
Mostacciuolo
G
G
D000818:Animals; D002118:Calcium / Q000502:physiology*; D005347:Fibroblasts / Q000502:physiology*; D066298:In Vitro Techniques; D007473:Ion Channels; D008564:Membrane Potentials; D051379:Mice
D007473:Ion Channels; D002118:Calcium