Histopathology of experimental Aspergillus fumigatus keratitis.
Histopathological studies in rabbit's eyes, 7 and 14 days after intracorneal inoculation with 1 X 10(5) Aspergillus fumigatus conidia have been performed. Similar lesions were found in both periods with fungal hyphae in the anterior third of corneal stroma, round cell infiltration from the sclero-corneal edge and in the anterior chamber and, neovascularization. No lesions were found in the Descemet's membrane. Gomori silver-methenamine stain with hematoxiline-eosine counter-stain was found to be the most reliable stain to detect fungal presence in corneal stroma, and Masson's trichromic stain in the study of pathological changes in ocular elements.
Fons
A
A
Departamento de Microbiología, Facultad de Medicina, Valencia, Spain.
García-de-Lomas
J
J
Nogueira
J M
JM
Buesa
F J
FJ
Gimeno
C
C
D000818:Animals; D001228:Aspergillosis / Q000382:microbiology / Q000473:pathology*; D001232:Aspergillus fumigatus / Q000302:isolation & purification*; D003315:Cornea / Q000473:pathology*; D003319:Corneal Stroma / Q000382:microbiology / Q000473:pathology*; D007634:Keratitis / Q000382:microbiology / Q000473:pathology*; D011817:Rabbits; D013194:Staining and Labeling