title: Endotoxin abolishes the induction of alpha 2-macroglobulin synthesis in cultured human monocytes indicating inhibition of the terminal monocyte maturation into macrophages.
abstract: Monocytes were prepared from human blood and allowed to differentiate into macrophages in vitro. Synthesis and secretion of the potent proteinase inhibitor alpha 2-macroglobulin is strongly induced during maturation of monocytes into macrophages establishing alpha 2-macroglobulin as a differentiation marker protein. When the monocytes were incubated with endotoxin (S. typhi) in a concentration as low as 100 ng/ml, induction of alpha 2-macroglobulin synthesis was completely abolished indicating impaired monocyte/macrophage differentiation by lipopolysaccharides (LPS). In contrast to alpha 2-macroglobulin, synthesis of alpha 1-proteinase inhibitor in mononuclear phagocytes was stimulated by endotoxin.
authors: Medizinische Universitätsklinik Freiburg, West Germany.
authors: J
authors: J
authors: Bauer
authors: U
authors: U
authors: Ganter
authors: W
authors: W
authors: Gerok
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D004731:Endotoxins / Q000494:pharmacology*; D006801:Humans; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D009000:Monocytes / Q000166:cytology / Q000378:metabolism*; D012485:Salmonella typhi; D000511:alpha-Macroglobulins / Q000037:antagonists & inhibitors / Q000096:biosynthesis
chemical_list: D004731:Endotoxins; D000511:alpha-Macroglobulins