Relationship between energy expenditure and ion channel density in the turtle and rat brain.
Synaptosomes were isolated from turtle and rat brains to determine whether differences in brain ion channel densities accounted for the turtle's ability to survive anoxia compared with the mammal. The Na(+)-channel binding neurotoxin brevetoxin showed high-affinity specific binding in both turtle and rat synaptosomes, suggesting specific ligand-receptor interaction. The maximum binding capacity (Bmax) value for the turtle was only about one-third of that found for the rat synaptosomes, suggesting that the turtle synaptosome has a correspondingly lower Na+ channel density than the rat. This apparent difference in Na+ channel density is not reflected in metabolic rates, since at the same temperature (31 degrees C) the O2 consumption of both the rat and turtle synaptosome was almost identical. The large reductions in energy expenditure seen in synaptosomes incubated in Na(+)-free media and in media containing ouabain (approximately 50% turtle, 80% rat) are probably related to the halting of transmembrane Na(+)-K+ exchange. The greater reduction in the rat may be related to the apparent greater density of Na+ channels in the rat brain. However, compared with the 90% reduction in brain metabolism that occurs when the turtle brain becomes anoxic, the differences in ion channel density and in the costs of ion pumping between the rat and turtle brain are trivial. Closing Na+ channels with tetrodotoxin and increasing Na+ channel activation with veratridine caused substantial decreases and increases in synaptosome energy consumption, respectively. This suggests that the modulation of ion channel conductance has a significant effect on metabolic cost and may be an important mechanism to reduce energy consumption and electrical activity in the anoxic turtle brain, while still maintaining ionic gradients.
Edwards
R A
RA
Division of Biology and Living Resources, Rosenstiel School of Marine and Atmospheric Science, University of Miami, Florida 33149.
Lutz
P L
PL
Baden
D G
DG
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D004141:Dinoflagellida; D004734:Energy Metabolism / Q000187:drug effects; D007473:Ion Channels / Q000502:physiology*; D007700:Kinetics; D008387:Marine Toxins / Q000378:metabolism; D009928:Organ Specificity; D010042:Ouabain / Q000494:pharmacology; D035964:Oxocins; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D015222:Sodium Channels / Q000502:physiology; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*; D013779:Tetrodotoxin / Q000494:pharmacology; D014426:Turtles; D014701:Veratridine / Q000494:pharmacology; D000095702:Polyether Toxins
D002352:Carrier Proteins; D007473:Ion Channels; D008387:Marine Toxins; D010042:Ouabain; D035964:Oxocins; D015222:Sodium Channels; D013779:Tetrodotoxin; D014701:Veratridine; C023790:tetrodotoxin-binding protein; C053342:brevetoxin; D000095702:Polyether Toxins