title: Antigen-specific proliferative responses to vesicular stomatitis virus following immunization of cattle with inactive virus.
abstract: Peripheral blood mononuclear cells (PBM) from four normal cows with no known exposure to vesicular stomatitis virus (VSV) were cultured with a New Jersey (NJ) serotype (Ogden) VSV that had been UV-irradiated and inactivated. PBM from these animals produced no detectable proliferative response when incubated with varying concentrations of VSV-NJ (Ogden) ranging from 10 ng to 10 micrograms protein/ml. Two of these cows were immunized with an experimental VSV-NJ vaccine and their PBM were tested at various intervals after immunization. PBM tested 14 days after the initial immunization produced readily detectable antigen-specific proliferative responses when cultured with UV-irradiated strains of VSV-NJ. Following a second immunization, lower concentrations of antigen were sufficient to stimulate the proliferative response and the magnitude of the proliferative response was increased. The responsiveness persisted for at least 6 months after these two immunizations. The specificity of the proliferative response was examined by comparing the responses stimulated by one VSV-Ind and four VSV-NJ serotype strains. The PBM from the immunized cows produced proliferative responses that were essentially specific for the VSV-NJ serotype antigens. In dose titrations, the VSV-NJ antigens were 300-1000-fold more effective than was the VSV-Ind antigen. Thus, persistent antigen-specific proliferative responsiveness that is serotype specific can be stimulated by immunizing cattle with an inactivated VSV vaccine.
authors: Department of Microbiology, University of Nevada-Reno 89557.
authors: D
authors: D
authors: Redelman
authors: S
authors: S
authors: Nichol
authors: R
authors: R
authors: Klieforth
mesh_terms: D000818:Animals; D002417:Cattle / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D007111:Immunity, Cellular; D007117:Immunization, Secondary; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D015164:Vaccines, Inactivated / Q000276:immunology; D018116:Vesiculovirus / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000939:Epitopes; D015164:Vaccines, Inactivated; D014765:Viral Vaccines