title: Autoradiographic localization of binding sites for 125I-substance P on neurones from cultured rat superior cervical ganglion.
abstract: Using an autoradiographic receptor binding technique, the distribution of substance P (SP) receptors on cells cultured from the superior cervical ganglia (SCG) of newborn rats was investigated. Binding sites for 125I-Bolton-Hunter-SP were observed on a subpopulation of 35-50% of the ganglion neurones. The percentage of labelled neurones remained constant whether the cultures were seeded densely or sparsely. Variation in the density of labelling was observed on different neuronal clusters. Neuronal cell bodies were often densely labelled, but neuronal processes were rarely labelled. In contrast with the neuronal cells, specific labelling was not associated with other cell types found in this culture preparation, including fibroblasts, glial cells and other non-neuronal supporting cells. These results are interpreted to suggest that there is a subpopulation of SCG neurones which, by virtue of their expressing SP receptors, are responsive to SP and have a physiological role within the ganglion.
authors: Department of Anatomy and Developmental Biology, University College London, U.K.
authors: S
authors: S
authors: James
authors: G
authors: G
authors: Burnstock
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001345:Autoradiography; D002478:Cells, Cultured; D005728:Ganglia, Sympathetic / Q000378:metabolism*; D007457:Iodine Radioisotopes; D009474:Neurons / Q000378:metabolism*; D051381:Rats; D018040:Receptors, Neurokinin-1; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D013373:Substance P / Q000378:metabolism*
chemical_list: D007457:Iodine Radioisotopes; D018040:Receptors, Neurokinin-1; D017981:Receptors, Neurotransmitter; D013373:Substance P