Sodium dodecyl sulfate-gel electrophoresis: staining of polypeptides using heavy metal salts.
Water-soluble salts of several heavy metals were examined for their ability to stain polypeptides resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Brief gel exposure (5 min or less) to cobaltous acetate or chlorides of copper, nickel, and zinc produced negatively stained protein patterns that were qualitatively indistinguishable from those of parallel gels stained with Coomassie blue R-250. Protein patterns could be visualized less than 1 min after treatment of gels with zinc chloride; the threshold of detection was estimated at about 10-12 ng protein on standard-size slab gels. Test samples including human erythrocyte membranes, sialoglycoprotein (glycophorin) extracts, and commercial molecular weight protein standards were used to establish the scope of these stains. Protein patterns visualized by the heavy metal salts were compared and contrasted with profiles seen with three widely used silver stains. Proteins from gels treated with copper or zinc chloride could be easily recovered by simple diffusion; this makes feasible both analytical and preparative electrophoretic applications of the staining procedure. A mechanism is proposed to explain the observed protein staining by heavy metal salts.
Dzandu
J K
JK
Department of Anatomy, Texas College of Osteopathic Medicine, Fort Worth 76107.
Johnson
J F
JF
Wise
G E
GE
D003300:Copper; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D008670:Metals; D010455:Peptides / Q000302:isolation & purification*; D012967:Sodium Dodecyl Sulfate; D013194:Staining and Labeling / Q000379:methods*; D015032:Zinc
D008670:Metals; D010455:Peptides; D012967:Sodium Dodecyl Sulfate; D003300:Copper; D015032:Zinc