Activation of the major late promoter in adenovirus transformed cells by 5-azacytidine.
The adenovirus major late promoter (MLP) is normally not active in transformed cells. We investigated if it could be activated with 5-azacytidine. Three days of treatment with 10 microM 5-azacytidine induced transient activation of the MLP as shown by hybridization with an L1 r-strand-specific probe. The po/III-transcribed VA-RNAs were not activated. L1 activation was not accompanied by detectable changes in methylation of HpaII sites at the promoter or in the body of the transcript. Stably activated cell clones could be obtained at 20% frequency after long-term drug treatment.
Robert
N
N
Département de Microbiologie, Université de Sherbrooke, Québec, Canada.
Toth
M
M
Tellier
R
R
Horvath
J
J
Déry
C V
CV
Weber
J M
JM
D000256:Adenoviridae / Q000187:drug effects* / Q000235:genetics; D000818:Animals; D001374:Azacitidine / Q000494:pharmacology*; D002460:Cell Line; D002472:Cell Transformation, Viral; D004279:DNA, Viral / Q000235:genetics; D008745:Methylation; D011401:Promoter Regions, Genetic / Q000187:drug effects*; D051381:Rats; D013268:Stimulation, Chemical; D014158:Transcription, Genetic / Q000187:drug effects
D004279:DNA, Viral; D001374:Azacitidine