Identification of a HeLa mRNA fraction which can correct the DNA-repair defect in Fanconi anaemia fibroblasts.
Injection of Fanconi anaemia (complementation group A) fibroblasts with HeLa mRNA is shown to correct their abnormal response to a psoralen cross-linking challenge, namely permanent repression of DNA synthesis. Injection of gradient-fractionated mRNA led to identification of a single fraction, containing mRNA of approximately 650 bases, which is responsible for this effect. This finding suggests that Fanconi anaemia (group A) cells are deficient in a small protein, up to 20 kDa in size, which is involved in the cellular response to DNA interstrand cross-links.
Digweed
M
M
Institut für Humangenetik, Freie Universität Berlin, Germany.
Sperling
K
K
D000225:Adenine / Q000378:metabolism; D000741:Anemia, Aplastic / Q000188:drug therapy*; D002460:Cell Line; D002675:Child, Preschool; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D004260:DNA Repair / Q000187:drug effects* / Q000528:radiation effects; D005199:Fanconi Anemia / Q000188:drug therapy* / Q000235:genetics; D005347:Fibroblasts; D005363:Ficusin / Q000037:antagonists & inhibitors / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D008297:Male; D008730:Methoxsalen / Q000378:metabolism; D008845:Microinjections; D012333:RNA, Messenger / Q000494:pharmacology*; D012194:RNA-Directed DNA Polymerase; D014466:Ultraviolet Rays
D003432:Cross-Linking Reagents; D012333:RNA, Messenger; D004247:DNA; D012194:RNA-Directed DNA Polymerase; D000225:Adenine; D005363:Ficusin; D008730:Methoxsalen