title: Immunoglobulin heavy H chain isotypes in a teleost fish.
abstract: Three mouse mAb were derived which identified different populations of the approximately 700,000-Da tetrameric Ig of channel catfish (Ictalurus punctatus). Immunoprecipitation analyses using the three mAb in various combinations showed that the catfish Ig population identified by each mAb was antigenically distinct. Each Ig population contained both classes of catfish L chains (F and G). Solid-phase binding assays indicated that the mAbs preferentially identified H chains rather than L chains. Comparative peptide mapping analyses of the H chains defined by each mAb indicated that H chains were different, although each had the same apparent mass (approximately 70,000 Da). This structural distinction was not based upon allotypic variation because the analysis of the serum from individual catfish showed the presence of each H chain in fish examined. Therefore, the different H chains represent different isotypes; each representing approximately 20% of the total serum Ig. There is at least one undefined additional H chain isotype found in the serum Ig population. Partial sequence information was obtained on the first variable framework region (FR1) of two of the three H chain isotypes. These results indicated that the variable region associated with each isotype was heterogeneous; two and sometimes three different residues were represented in the majority of positions. The sequence information also showed that the FR1 of one H chain was distinct from the other; there was less than a 50% match of the primary residues. The analyses suggest that the V region genes which code for the FR1 are differently associated with each H chain isotype. Finally, the relative levels of the three H chain isotypes were monitored during the temporal immune response of individual catfish to the dinitrophenyl hapten. These results indicated that one H chain isotype was preferentially expressed early in the immune response (approximately 3 wk) and remained the predominantly expressed isotype during the humoral immune response.
authors: Department of Microbiology, University of Mississippi Medical Center, Jackson 39216-4505.
authors: C J
authors: CJ
authors: Lobb
authors: M O
authors: MO
authors: Olson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D002397:Catfishes / Q000276:immunology*; D004136:Dinitrobenzenes / Q000276:immunology; D000939:Epitopes / Q000276:immunology / Q000302:isolation & purification; D006241:Haptens / Q000276:immunology; D007059:Ictaluridae / Q000276:immunology*; D007143:Immunoglobulin Heavy Chains / Q000276:immunology / Q000302:isolation & purification*; D007132:Immunoglobulin Isotypes / Q000276:immunology / Q000302:isolation & purification*; D007135:Immunoglobulin Variable Region / Q000302:isolation & purification; D051379:Mice; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010802:Phylogeny
chemical_list: D000911:Antibodies, Monoclonal; D004136:Dinitrobenzenes; D000939:Epitopes; D006241:Haptens; D007143:Immunoglobulin Heavy Chains; D007132:Immunoglobulin Isotypes; D007135:Immunoglobulin Variable Region