Temperature gradient DNA-probe column chromatography: as tools for detection and purification of particular DNAs and RNAs.
We have developed the temperature-gradient DNA-probe column chromatography as the new method for detecting and purifying particular DNAs or RNAs accurately. The method has high discrimination resolution, and can detect a single-base change in sample base sequences. The method also has high performances in purification of particular DNAs or RNAs, which has been demonstrated by purification of bovine mitochondria serine t-RNA. None of other method can succeed in complete purification of this t-RNA molecule. The present method can be applied not only to molecular biology as basic tools for purifying and picking-up particular sequences but also to the medical field as diagnostic tools.
Tsurui
H
H
Department of Physics, Faculty of Science, University of Tokyo, Japan.
Suyama
A
A
Wada
A
A
D001483:Base Sequence; D002846:Chromatography, Affinity / Q000379:methods; D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D015342:DNA Probes; D009690:Nucleic Acid Conformation; D012313:RNA / Q000032:analysis* / Q000302:isolation & purification; D013696:Temperature
D015342:DNA Probes; D012313:RNA; D004247:DNA