Influence of phosphatidylcholine on two protein mRNA levels in interferon treated Daudi cells.
Evidence related to nuclear structure and metabolism have shown the presence of a chromatin lipid fraction which may be involved in the events of cell proliferation and differentiation (Maraldi et al., 1984, Cocco et al., 1987). The relative amounts of these molecules have been reported to be modified in different metabolic conditions of normal and neoplastic cells (Coetzee et al., 1975; Manzoli et al., 1982). Phospholipids have also been found among the components of the nuclear matrix where they seem to play a role in the interactions between nucleic acids and proteins (Berezney, 1984; Cocco et al., 1987). In this paper we report that the transcriptional induction of two genes (pIFN-IND-1 and pIFN-IND-2) produced by interferon in Daudi lymphoma cells is influenced by addition, in the transcriptional assay, of phosphatidylcholine (PC) liposomes which have been reported to be able to enter nuclei (Maraldi et al., 1984; Maraldi et al., 1982).
Miscia
S
S
Institute of Human Morphology, University of Chieti, Italy.
Cataldi
A
A
Grimley
P M
PM
D002460:Cell Line; D004337:Drug Carriers; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007372:Interferons / Q000008:administration & dosage / Q000494:pharmacology*; D008081:Liposomes; D008223:Lymphoma / Q000378:metabolism / Q000473:pathology*; D010713:Phosphatidylcholines / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects; D014407:Tumor Cells, Cultured
D004337:Drug Carriers; D008081:Liposomes; D010713:Phosphatidylcholines; D012333:RNA, Messenger; D007372:Interferons