Effects of recombinant interleukin 1 and interleukin 2 on human keratinocytes.
The effects of recombinant interleukin 1 alpha and beta, as well as recombinant interleukin 2, on human keratinocyte proliferation were studied in serum-containing as well as defined media. Both interleukin 1 preparations did not stimulate keratinocyte growth; interleukin 2 also did not stimulate keratinocyte growth. To determine whether interleukin 1 beta binds to keratinocytes, a cell membrane assay was developed for these cells. Iodinated interleukin 1 beta binds to keratinocytes with a kD of 6.2 nm and 2500 receptors per cell. To determine the effects of interleukin 1 beta on protein synthesis, the molecular patterns of radiolabeled cell extracts of interleukin 1 beta-treated and nontreated keratinocytes were compared using two-dimensional polyacrylamide gel electrophoresis. No significant changes in the molecular pattern of newly synthesized proteins were detected. Finally, none of these lymphokines induced HLA-DR expression by keratinocytes.
Morhenn
V B
VB
Department of Dermatology, Stanford University Medical Center, Palo Alto, California.
Wastek
G J
GJ
Cua
A B
AB
Mansbridge
J N
JN
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D000078404:Epidermal Cells; D004817:Epidermis / Q000276:immunology / Q000378:metabolism; D006684:HLA-DR Antigens / Q000032:analysis / Q000145:classification; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism / Q000494:pharmacology*; D007376:Interleukin-2 / Q000494:pharmacology*; D007633:Keratins; D014176:Protein Biosynthesis; D011994:Recombinant Proteins
D006684:HLA-DR Antigens; D007375:Interleukin-1; D007376:Interleukin-2; D011994:Recombinant Proteins; D007633:Keratins