Basophilic bodies of skeletal muscle in hypothyroidism: enzyme histochemical and ultrastructural studies.
Basophilic bodies of skeletal muscles from two patients with hypothyroidism were examined by enzyme histochemistry and ultrastructural study of ultrathin sections stained with periodic-acid-thiocarbohydrazide-silver proteinate for polysaccharides. Some additional characterizations of basophilic bodies were observed: basophilic bodies were found exclusively in type 1 fiber; basophilic bodies were devoid of myofibrillary adenosine triphosphatase, oxidative enzymes, and phosphorylase; and both fibrillary and granular components of basophilic bodies stained strongly for polysaccharides. The polysaccharide nature of basophilic bodies is in keeping with the previous suggestion that the formation of basophilic bodies in hypothyroid patients is related to an impairment of carbohydrate metabolism. Their selective involvement of type 1 fiber and preferential occurrence at the myotendinous junction remain obscure.
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Department of Pathology, Henry Ford Hospital, Detroit, MI 48202.
D000251:Adenosine Triphosphatases / Q000032:analysis; D000368:Aged; D004396:Coloring Agents; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D006834:Hydrazines; D007037:Hypothyroidism / Q000473:pathology*; D008854:Microscopy, Electron; D009132:Muscles / Q000201:enzymology / Q000473:pathology*; D009210:Myofibrils / Q000201:enzymology; D010504:Periodic Acid; D006005:Phosphorylases / Q000032:analysis; D011134:Polysaccharides / Q000032:analysis; D012836:Silver Proteins; D013194:Staining and Labeling
D004396:Coloring Agents; D006834:Hydrazines; D011134:Polysaccharides; D012836:Silver Proteins; C055835:periodic acid-thiocarbohydrazide-silver proteinate; D010504:Periodic Acid; D006005:Phosphorylases; D000251:Adenosine Triphosphatases