title: Identification of a German cockroach-specific allergen by human IgE and rabbit IgG.
abstract: To further study the immunology of insect hypersensitivity, we identified and partially characterized the principal allergens in whole body German cockroach (WBGCR) (Blattella germanica) and compared this extract to whole body antigens prepared from other insects. WBGCR extract was fractionated over a calibrated Sephadex G-200 column; peak allergenic activity was contained in fraction 3 (GCR3), containing components with apparent molecular weights ranging from 12,500 to 75,000 daltons. The antigenicity, allergenicity, and specificity of GCR3 components were tested by using rabbit antisera raised to GCR3 or true armyworm (Pseudaletia unipuncta). Radioimmunoassay, cross-inhibition and immunoblot studies revealed, particularly in the IgE system, that GCR3 contained an allergen with a pI of 6.7 and MW of 36,000 daltons that was unique to WBGCR extracts and not present in other insect species, including true armyworm, caddis fly, lakefly, yellow jacket, or honeybee. This GCR3 component may represent a specific marker for the diagnosis of cockroach hypersensitivity in an insect-sensitive population of individuals.
authors: Department of Immunology, Mayo Medical School, Rochester, Minn.
authors: R M
authors: RM
authors: Helm
authors: E O
authors: EO
authors: Bandele
authors: M C
authors: MC
authors: Swanson
authors: A R
authors: AR
authors: Campbell
authors: S R
authors: SR
authors: Wynn
mesh_terms: D000485:Allergens / Q000032:analysis* / Q000276:immunology; D000818:Animals; D000937:Antigen-Antibody Reactions; D001667:Binding, Competitive; D002850:Chromatography, Gel; D003058:Cockroaches / Q000276:immunology*; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D007073:Immunoglobulin E; D007074:Immunoglobulin G; D007525:Isoelectric Focusing; D011817:Rabbits; D011852:Radioallergosorbent Test
chemical_list: D000485:Allergens; D000939:Epitopes; D007074:Immunoglobulin G; D007073:Immunoglobulin E