Improved avidin-biotin-peroxidase complex (ABC) staining.
A considerable intensification of the avidin-biotin-peroxidase complex staining system (ABC) was obtained by sequentially overlaying the sections to be immunostained with an avidin-rich and a biotin-rich complex. Each sequential addition contributed to the deposition of horseradish peroxidase on the immunostained site and allowed the subsequent binding of a complementary complex. With this technique a higher dilution of the antisera could be used and minute amounts of antigen masked by the fixative could be demonstrated on paraffin sections.
Cattoretti
G
G
Division of Anatomic Pathology and Cytology, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milano, Italy.
Berti
E
E
Schiró
R
R
D'Amato
L
L
Valeggio
C
C
Rilke
F
F
D001360:Avidin; D001710:Biotin; D006651:Histocytochemistry; D006735:Horseradish Peroxidase; D006801:Humans; D010544:Peroxidases; D013194:Staining and Labeling / Q000379:methods*
D001360:Avidin; D001710:Biotin; D006735:Horseradish Peroxidase; D010544:Peroxidases