The use of functional analysis of the ribosome as a tool to determine archaebacterial phylogeny.
Forty different antibiotics with diverse kingdom and functional specificities were used to measure the functional characteristics of the archaebacterial translation apparatus. The resulting inhibitory curves, which are characteristic of the cell-free system analyzed, were transformed into quantitative values that were used to cluster the different archaebacteria analyzed. This cluster resembles the phylogenetic tree generated by 16S rRNA sequence comparisons. These results strongly suggest that functional analysis of an appropriate evolutionary clock, such as the ribosome, is of intrinsic phylogenetic value. More importantly, they indicate that the study of the nexus between genotypic and phenotypic (functional) information may shed considerable light on the evolution of the protein synthetic machinery.
Amils
R
R
Centro de Biología Molecular UAM-CSIC, Cantoblanco, Madrid, Spain.
Ramírez
L
L
Sanz
J L
JL
Marín
I
I
Pisabarro
A G
AG
Ureña
D
D
D001105:Archaea / Q000235:genetics* / Q000378:metabolism; D001419:Bacteria / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics; D005057:Eukaryotic Cells / Q000378:metabolism; D010802:Phylogeny; D014176:Protein Biosynthesis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012270:Ribosomes / Q000378:metabolism*
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S