title: Augmentation of bleomycin-induced DNA damage in intact cells.
abstract: Bleomycin, an important cause of pulmonary fibrosis, is known to produce DNA damage. The mechanism for this damage in vitro is related to free radical production by a bleomycin and iron complex. To determine whether bleomycin causes damage to DNA in vivo by a similar mechanism, we used a viral minichromosome that is replicated in cultured cells. Bleomycin causes dose-dependent damage to intracellular DNA, and this damage is augmented by Fe2+ but not Fe3+. The augmentation of the bleomycin-induced DNA damage caused by Fe2+ is also dose dependent in that increasing DNA damage occurs with increasing amounts of Fe2+. These studies demonstrate that bleomycin causes damage to DNA in vivo and suggest that bleomycin must rely on Fe2+ to donate an electron for oxygen radical-induced DNA strand scission.
authors: Department of Internal Medicine, University of Iowa College of Medicine and Hospitals, Iowa City 52242.
authors: P L
authors: PL
authors: Moseley
mesh_terms: D000818:Animals; D001761:Bleomycin / Q000494:pharmacology*; D002460:Cell Line; D002477:Cells / Q000187:drug effects*; D004249:DNA Damage; D005290:Ferric Compounds / Q000494:pharmacology; D005296:Ferrous Compounds / Q000494:pharmacology
chemical_list: D005290:Ferric Compounds; D005296:Ferrous Compounds; D001761:Bleomycin