[Change in nucleic acid synthesis in the spleen of rats with acute deficit of vitamin B1 during immunogenesis].
After four administrations of human blood serum gamma-globulin (intraperitoneally, once during 4 days at a dose of 1 mg and the following reimmunization within 60 days at a dose of 3-4 mg) distinct incorporation of 14C-thymidine (specific activity 54 muCi/mumole, by 5 muCi per an animal, 2 hrs before decapitation, intraperitoneally) into rat spleen DNA was detected within 4 days of immunogenesis. Acute oxythiamine B1 avitaminosis (single subcutaneous administration of oxythiamine at a dose of 400 mg/kg) led to a decrease in the label incorporation into spleen DNA of immunized rats by 70% (p less than 0.01) as compared with control immunized animals, while incorporation of 14C-thymidine was unaltered in intact rats. Content of RNA was not distinctly altered in spleen of B1-deficient rats within the 4 days of immunogenesis. Acute deficiency of vitamin B1 thus impaired synthesis of DNA in spleen during development of immunogenesis.
Dvorianinovich
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LN
D000818:Animals; D000917:Antibody Formation; D004247:DNA / Q000032:analysis / Q000096:biosynthesis* / Q000187:drug effects; D007114:Immunization; D008297:Male; D010119:Oxythiamine / Q000494:pharmacology; D012313:RNA / Q000032:analysis / Q000096:biosynthesis* / Q000187:drug effects; D051381:Rats; D013154:Spleen / Q000201:enzymology / Q000378:metabolism*; D013832:Thiamine Deficiency / Q000201:enzymology / Q000276:immunology / Q000378:metabolism*; D014174:Transketolase / Q000378:metabolism; D005719:gamma-Globulins / Q000008:administration & dosage
D005719:gamma-Globulins; D010119:Oxythiamine; D012313:RNA; D004247:DNA; D014174:Transketolase
Izmenenie sinteza nukleinovykkh kislot v selezenke krys s ostrym defitsitom vitamina B1 pri immunogeneze.