Effects of substance P on the spontaneous binding of Salmonella minnesota R345 (Rb) to human peripheral blood lymphocytes.
The effects of substance P (SP) on Salmonella minnesota R345 (Rb) binding to human peripheral blood lymphocytes (PBL) were evaluated. Two parameters of bacterial cytoadherence were considered, namely the binding lymphocytes (BL) and the number of bound-bacteria/lymphocyte (BB). The results showed that SP inhibits both BL and BB in a significant manner. Furthermore, distribution of Salmonella binding to CD4+ and CD8+ lymphocytes was studied following SP pretreatment of lymphoid cells. This neuropeptide is able to hamper the bacterial cytoadherence to both T-cell subpopulations and, in particular, the inhibitory effect on the T-suppressor/cytotoxic subset was more pronounced. These findings are discussed in terms of SP intervention in the mechanism of host protection against invading microorganisms.
De Simone
C
C
Malattie Infettive, University of L'Aquila degli Abruzzi, Italy.
Misefari
A
A
Covelli
V
V
Maffione
A B
AB
Antonaci
S
S
Jirillo
E
E
D015703:Antigens, CD / Q000032:analysis; D001422:Bacterial Adhesion / Q000187:drug effects*; D001665:Binding Sites / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000382:microbiology; D012475:Salmonella / Q000187:drug effects* / Q000472:pathogenicity; D013373:Substance P / Q000494:pharmacology*
D015703:Antigens, CD; D013373:Substance P