[Immuno-morphologic detection of specific APO-B antigenic determinants in normal intima and atherosclerotic plaques in the human aorta].
Various apo-B antigenic determinants exposures in the normal aortic intima and atherosclerotic plaques were examined by immunofluorescence using 5 clones from monoclonal antibodies (BAb) to apo-B. As a rule, 4 clones were found to react with apo-B in the normal aortic intima, whereas the clone 12G10 failed to respond to it. All the 5 determinants of apo-B were exposed in the fibrous tissue of most atherosclerotic plaques. However, there were atherosclerotic plaques, in whose fibrous tissue only did 1 to 3 clones positively react with apo-B. The similar expression of apo-B antigenic determinants was observed in the atheronecrotic zone of fibrous plaques. Apo-B did not respond to apo-B MAb at all in some atherosclerotic plaques present in the zone of atheronecrosis. Whether it is possible to modify low-density lipoproteins just in the arterial wall, especially in atherosclerotic plaques and whether this process is significant in the pathogenesis of atherosclerosis are discussed in the paper.
Shekhonin
B V
BV
Tararak
E M
EM
Kovaleva
E A
EA
Ianushevskaia
E V
EV
Trakht
I N
IN
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D001011:Aorta / Q000276:immunology*; D001055:Apolipoproteins B / Q000276:immunology*; D001161:Arteriosclerosis / Q000276:immunology*; D000939:Epitopes / Q000032:analysis*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism; D008297:Male; D008875:Middle Aged
D000911:Antibodies, Monoclonal; D001055:Apolipoproteins B; D000939:Epitopes; D008077:Lipoproteins, LDL
Immunomorfologicheskoe vyiavlenie otdel'nykh antigennykh determinant apoB v normal'noĭ intime i ateroskleroticheskikh bliashkakh aorty cheloveka.