The transformation-sensitive heat shock protein (hsp47) binds specifically to Fetuin.
The transformation-sensitive heat shock protein of Mr = 47,000 (hsp47) has been shown to bind to collagen and gelatin. We examined the binding specificity of hsp47. The binding of hsp47 to gelatin-Sepharose 4B was competitively inhibited by fetuin as effectively as by gelatin or collagen, whereas a variety of other proteins tested had no effect. Fetuin-coupled Sepharose 4B was found to bind hsp47 even at high ionic strength, but the complex was dissociated at pH less than or equal to 5.5.)
Nakai
A
A
Chest Disease Research Institute, Kyoto University, Japan.
Hirayoshi
K
K
Saga
S
S
Yamada
K M
KM
Nagata
K
K
D000818:Animals; D001667:Binding, Competitive; D002642:Chick Embryo; D002846:Chromatography, Affinity; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D000509:alpha-Fetoproteins / Q000378:metabolism*
D006360:Heat-Shock Proteins; D000509:alpha-Fetoproteins