Outer membrane proteins of Yersinia: major protein induced by maltose.
The composition of outer membrane (OM) proteins of Y. enterocolitica, Y. intermedia, Y. frederiksenii and Y. kristensenii was investigated. POMP proteins were described from Y. enterocolitica and Y. intermedia. In all the studied bacteria the presence of two to four major proteins, that is YOMP-C, YOMP-F, YOMP-A and protein with molecular weight 47 kDa was demonstrated. The position of 47 kDa protein on polyacrylamide gel (SDS-PAGE) corresponds to maltoporins in Escherichia coli. This protein may be induced by the addition of maltose to the medium, and in the case of Y. intermedia also maltodextrins. The amount of 47 kDa protein in the OM of all the examined strains does not change after addition of Ca++ ions, it increases, however, under conditions of increased osmolarity. Y. enterocolitica is an exception since its synthesis of the above mentioned protein is independent on the medium osmolarity.
Bielecki
J
J
Institute of Microbiology, Warsaw University, Warszawa, Poland.
Hrebenda
J
J
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D008320:Maltose / Q000494:pharmacology*; D014774:Virulence; D015007:Yersinia / Q000032:analysis* / Q000472:pathogenicity
D001425:Bacterial Outer Membrane Proteins; D008320:Maltose