Decrease of atrial natriuretic peptide content in rat superior cervical sympathetic ganglion after denervation and axotomy.
We found atrial natriuretic peptide (ANP), known as a humoral factor in regulating body fluid volume and blood pressure, in considerable quantities in rat superior cervical sympathetic ganglion (SCG) by radioimmunoassay after separation with reverse-phase HPLC. Although the ANP content of the immature rat 1 week after birth was low, it doubled at 2 weeks and then increased gradually, until it reached the adult level. Denervation caused a rapid decrease in the ANP content to half of the intact SCG level after 3 h, which then fell to 10% of the control value on day 2 after operation. The time course of ANP content reduction after denervation was similar but rather faster than that of activity of the acetylcholine-synthesizing enzyme, choline acetyltransferase, an observation suggesting that ANP may partly contribute to cholinergic synaptic transmission. On the other hand, axotomy produced a rather slower decrease in the ANP content than did denervation. Enucleation and sialoadenectomy also caused a considerable reduction of the ANP content. Thus, part of the ANP found in the ganglion is apparently transported from sympathetically innervated extraganglionic organs via retrograde axoplasmic flow.
Nagata
Y
Y
Department of Physiology, School of Medicine, Fujita-Gakuen Health University; Aichi, Japan.
Ebisu
H
H
Tamaru
M
M
Fujita
K
K
Koide
T
T
D000375:Aging; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D001370:Axonal Transport; D001369:Axons / Q000502:physiology*; D001921:Brain / Q000378:metabolism; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003714:Denervation; D005123:Eye / Q000294:innervation; D005260:Female; D005728:Ganglia, Sympathetic / Q000254:growth & development / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012469:Salivary Glands / Q000294:innervation
D009320:Atrial Natriuretic Factor; D002795:Choline O-Acetyltransferase