A polyomavirus tumor-specific transplantation antigen (TSTA) epitope is situated within the N-terminal amino acid sequence common to middle and small T-antigens.
An MT cDNA-transformed rat cell line (2.8), that contains only the polyoma middle T-antigen and expresses polyoma TSTA, can be rejected in rats immunized with 1837 cells that carry a host range (A185 hr-t), mutant which expresses a full-length large T-antigen, and nonfunctional N-terminal fragments of small and middle T. This shows that at least one polyoma TSTA epitope is situated in the 113 amino acid N-terminal region, which is common to middle and small T-antigens.
Reinholdsson
G
G
Department of Tumor Biology, Karolinska Institute, Stockholm, Sweden.
Ramqvist
T
T
Brandberg
J
J
Dalianis
T
T
D000595:Amino Acid Sequence; D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics / Q000276:immunology*; D000954:Antigens, Surface / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics; D000939:Epitopes; D007114:Immunization; D009374:Neoplasms, Experimental / Q000235:genetics / Q000382:microbiology; D011120:Polyomavirus / Q000235:genetics / Q000276:immunology*; D051381:Rats
D000952:Antigens, Polyomavirus Transforming; D000954:Antigens, Surface; D004279:DNA, Viral; D000939:Epitopes