Signal peptide for protein secretion directing glycophospholipid membrane anchor attachment.
Decay accelerating factor (DAF) is anchored to the plasma membrane by a glycophospholipid (GPI) membrane anchor covalently attached to the COOH-terminus of the protein. A hydrophobic domain located at the COOH-terminus is required for anchor attachment; DAF molecules lacking this domain are secreted. Replacement of the COOH-terminal hydrophobic domain with a signal peptide that normally functions in membrane translocation, or with a random hydrophobic sequence, results in efficient and correct processing, producing GPI-anchored DAF on the cell surface. The structural requirements for GPI anchor attachment and for membrane translocation are therefore similar, presumably depending on overall hydrophobicity rather than specific sequences.
Caras
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IW
Genentech, South San Francisco, CA 94080.
Weddell
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GN
D000595:Amino Acid Sequence; D001798:Blood Proteins; D018958:CD55 Antigens; D050260:Carbohydrate Metabolism; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D003169:Complement Inactivator Proteins; D019856:Ethanolamine; D004983:Ethanolamines / Q000378:metabolism; D013006:Growth Hormone; D006801:Humans; D007163:Immunosorbent Techniques; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D010716:Phosphatidylinositols / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D021382:Protein Sorting Signals / Q000502:physiology*; D013329:Structure-Activity Relationship; D014162:Transfection
D001798:Blood Proteins; D018958:CD55 Antigens; D003169:Complement Inactivator Proteins; D004983:Ethanolamines; D008565:Membrane Proteins; D010716:Phosphatidylinositols; D010743:Phospholipids; D021382:Protein Sorting Signals; D019856:Ethanolamine; D013006:Growth Hormone; D010727:Phosphoric Diester Hydrolases; D043265:Phosphatidylinositol Diacylglycerol-Lyase