Nucleic acid sequence relationships between enterovirus serotypes.
Forty-eight different enterovirus serotypes were analysed by a nucleic-acid hybridization test using probes derived from the 3' end of coxsackievirus A21 (CA21) and B3 (CB3), poliovirus 3 (P3) and enterovirus 70 (E70). More than 90% of the serotypes could be detected with this collection of reagents. The CB3 probe reacted with all the coxsackie B viruses, with all three poliovirus serotypes, and with almost all of the 30 ECHO virus types tested. In addition some of the coxsackie A viruses and the BrDr 73 strain of enterovirus 71 gave a positive signal with this probe. The P3 probe detected all the poliovirus strains and also some coxsackievirus A isolates but no coxsackie B or ECHO viruses. A similar hybridization pattern as with the P3 probe was obtained when the CA 21 probe was used. The E70 probe appeared to be strain-specific. The results indicate that nucleic-acid hybridization is a useful method for rapid detection and subgrouping of enteroviruses during virus isolation, and that the test could be further developed for typing of the strains.
Hyypiä
T
T
Department of Virology, University of Turku, Finland.
Maaronen
M
M
Auvinen
P
P
Stålhandske
P
P
Pettersson
U
U
Stanway
G
G
Hughes
P
P
Ryan
M
M
Almond
J
J
Stenvik
M
M
D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D004770:Enterovirus / Q000145:classification* / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000032:analysis; D012703:Serotyping
D012313:RNA; D004247:DNA