Cultivation of bluetongue virus-specific ovine T cells and their cross-reactivity with different serotype viruses.
Bluetongue (BT) virus-specific ovine T-cell lines were prepared from BT virus-infected sheep by three cycles of alternate stimulation and resting culture in vitro. In antigen-specific proliferation assays and/or cytotoxicity assays, most of these T-cell lines responded not only to homologous serotype virus but also heterologous serotype viruses. This cross-reactivity did not correlate with the relatedness of serotypes as defined by cross-neutralizing antibodies. One cell line, 58-014, has grown continuously for more than 7 months without loss of antigen specificity. However, most of the cell lines lost their antigen specificity 2-4 months after cultivation. Certain BT virus-specific T-cell lines were able to reduce BT virus replication in autologous skin fibroblast cell culture.
Takamatsu
H
H
Department of Immunology, Pirbright Laboratory, AFRC Institute for Animal Health, Pirbright, Surrey, U.K.
Jeggo
M H
MH
D000818:Animals; D001820:Bluetongue virus / Q000145:classification / Q000276:immunology*; D002460:Cell Line; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D008213:Lymphocyte Activation; D012087:Reoviridae / Q000276:immunology*; D012703:Serotyping; D012756:Sheep; D013601:T-Lymphocytes / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D000939:Epitopes