The use of dwell time cross-correlation functions to study single-ion channel gating kinetics.
The derivation of cross-correlation functions from single-channel dwell (open and closed) times is described. Simulation of single-channel data for simple gating models, alongside theoretical treatment, is used to demonstrate the relationship of cross-correlation functions to underlying gating mechanisms. It is shown that time irreversibility of gating kinetics may be revealed in cross-correlation functions. Application of cross-correlation function analysis to data derived from the locust muscle glutamate receptor-channel provides evidence for multiple gateway states and time reversibility of gating. A model for the gating of this channel is used to show the effect of omission of brief channel events on cross-correlation functions.
Ball
F G
FG
Department of Mathematics, University of Nottingham, United Kingdom.
Kerry
C J
CJ
Ramsey
R L
RL
Sansom
M S
MS
Usherwood
P N
PN
D007473:Ion Channels / Q000378:metabolism*; D007700:Kinetics; D008433:Mathematics; D008962:Models, Theoretical; D013997:Time Factors
D007473:Ion Channels