title: Purification, cloning and nucleotide sequence determination of cynomolgus monkey apolipoprotein C-II: comparison to the human sequence.
abstract: We have purified apolipoprotein C-II (apo C-II) from cynomolgus monkey plasma, prepared antibody against it and used the antibody to isolate a cDNA containing the complete coding sequence for cynomolgus monkey apo C-II. Sequence analysis indicated that the monkey apo C-II cDNA was 200 bp longer than the human and the difference in size was all in the 5 degrees untranslated region of mRNA. This was confirmed by Northern analysis of human and monkey RNA. There was an open reading frame in the monkey apo C-II cDNA sequence encoding a preprotein of 101 amino acids - identical in size to the human protein. The carboxyl terminal 44 amino acids of the protein were 100% homologous to the human apo C-II amino acid sequence indicating evolutionary conservation of both structure and function. However, the amino terminal 35 amino acids of the protein were only 75% homologous and the amino terminal 19 amino acids were only 58% homologous to the human sequence. The amino acid sequence derived from the nucleotide sequence predicts a more basic protein than the human apo C-II and this is confirmed by isoelectric focusing and immunoblotting.
authors: Molecular Biology Research, Upjohn Company, Kalamazoo, MI 49007.
authors: B E
authors: BE
authors: Whitted
authors: C K
authors: CK
authors: Castle
authors: H G
authors: HG
authors: Polites
authors: G W
authors: GW
authors: Melchior
authors: K R
authors: KR
authors: Marotti
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D053304:Apolipoprotein C-II; D001056:Apolipoproteins C / Q000032:analysis / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000302:isolation & purification; D006801:Humans; D008252:Macaca fascicularis; D008969:Molecular Sequence Data; D012313:RNA / Q000032:analysis / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid
chemical_list: D053304:Apolipoprotein C-II; D001056:Apolipoproteins C; D012313:RNA; D004247:DNA