Inhibition by lysosomal enzymes and mannose-6-phosphate of the phosphorylation of the lysosomal enzyme binding receptor protein from monkey brain.
The lysosomal enzymes beta-glucuronidase and alpha-L-fucosidase and mannose-6-phosphate inhibited the phosphorylation of the lysosomal enzyme binding receptor protein prepared from monkey brain. Inhibition of both serine and tyrosine phosphorylation was observed. A non-lysosomal glycoprotein enzyme butyrylcholinesterase, mannose or glucose did not inhibit phosphorylation. Tyrosine phosphorylation of histone by the receptor protein was also inhibited by the lysosomal enzymes and mannose-6-phosphate.
Panneerselvam
K
K
Neurochemistry Laboratory, Christian Medical College Hospital, Vellore, India.
Balasubramanian
A S
AS
D000818:Animals; D001921:Brain / Q000378:metabolism; D002091:Butyrylcholinesterase / Q000494:pharmacology; D005966:Glucuronidase / Q000494:pharmacology; D000882:Haplorhini; D006600:Hexosephosphates / Q000494:pharmacology*; D006657:Histones / Q000378:metabolism; D007335:Insulin-Like Growth Factor II / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism; D008247:Lysosomes / Q000201:enzymology / Q000378:metabolism*; D008360:Mannosephosphates / Q000494:pharmacology*; D008970:Molecular Weight; D010766:Phosphorylation; D019000:Phosphotyrosine; D017527:Receptor, IGF Type 2; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017451:Receptors, Somatomedin; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism; D005644:alpha-L-Fucosidase / Q000494:pharmacology
D006600:Hexosephosphates; D006657:Histones; D008360:Mannosephosphates; D017527:Receptor, IGF Type 2; D011956:Receptors, Cell Surface; D017451:Receptors, Somatomedin; D019000:Phosphotyrosine; C027693:mannose-6-phosphate; D014443:Tyrosine; D007335:Insulin-Like Growth Factor II; D002091:Butyrylcholinesterase; D005966:Glucuronidase; D005644:alpha-L-Fucosidase