Characteristics of ingress and life span of neutrophils at a site of acute inflammation, determined with the Sephadex model in rats.
Neutrophils labelled in vivo with 3H-thymidine accumulate in subcutaneously injected Sephadex, which causes an acute inflammation. Cells die and disintegrate within the Sephadex implants, leaving behind labelled nuclear fragments representing the number of dead cells. The total of immigrated cells and their life span can be calculated from the number of intact cells along with disintegrated cells found in a Sephadex implant. Intensive neutrophil ingress starts after 5 h, and after 6.5 h reaches a maximum of 52 x 10(6) cells per hour. After 11 h, a total of 137 x 10(6) cells has entered a Sephadex implant. The life span varies during the course of the experiment. The shortest life span of 20 min was measured during the period of intensive neutrophil ingress 5.5 h after onset of inflammation. Cellular death and disintegration is evidently due to self-destruction of the cells by peroxidation.
Egger
G
G
Universitätsinstitut für Funktionelle Pathologie, Graz, Austria.
Spendel
S
S
Porta
S
S
D000818:Animals; D002452:Cell Count; D002465:Cell Movement; D002470:Cell Survival; D003911:Dextrans; D007249:Inflammation / Q000473:pathology*; D008297:Male; D009504:Neutrophils / Q000502:physiology*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D003911:Dextrans; C025614:sephadex