Three antibodies to human intestinal brush border membrane are characterized as anti-Le(a).
In the course of raising monoclonal antibodies to human colonic and small intestinal epithelium, three were isolated which recognized epitopes on many colonic glycoproteins and many jejunal glycoproteins taken from some individuals, but not on the same glycoproteins from others. The specificities of these antibodies were examined by carrying out inhibitions of the binding of the antibodies to the jejunal glycoproteins by blood group substances and purified oligosaccharides. The glycoproteins were separated by electrophoresis and transferred to nitrocellulose strips which were then used for the inhibition experiments. The results indicated that the antibodies bind to Le(a)-active structures. The conditions were determined for achieving red cell agglutination by two of the antibodies.
Dickson
L
L
MRC Human Biochemical Genetics Unit, Galton Laboratory, University College, London, U.K.
Richman
P I
PI
Shaw
M A
MA
Donald
A S
AS
Swallow
D M
DM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D001667:Binding, Competitive; D002240:Carbohydrate Sequence; D003106:Colon / Q000276:immunology*; D000939:Epitopes / Q000032:analysis; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D007114:Immunization; D015151:Immunoblotting; D007413:Intestinal Mucosa / Q000276:immunology*; D007983:Lewis Blood Group Antigens / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008871:Microvilli / Q000276:immunology; D008969:Molecular Sequence Data
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006023:Glycoproteins; D007983:Lewis Blood Group Antigens