Nuclear and cytoplasmic sites for anti-sense control.
In transgenic tobacco, anti-sense control of the chimaeric bar (bialophos resistance) gene is manifested by a reduced bar transcript level and a reduced synthesis of bar translational product (PAT, phosphinothricin acetyl transferase) per transcript (1). This study shows that the anti-bar gene controls the transcript level and the translation efficiency of the bar mRNA through independent events and at different cellular sites. On the basis of these results a model for the mechanism of anti-sense control is proposed.
Cornelissen
M
M
Plant Genetic Systems NV, Gent, Belgium.
D002467:Cell Nucleus / Q000502:physiology; D046508:Culture Techniques; D003593:Cytoplasm / Q000502:physiology; D005786:Gene Expression Regulation; D017343:Genes, Plant; D005809:Genes, Regulator; D010947:Plants, Toxic; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics*; D018075:RNA, Complementary; D012333:RNA, Messenger / Q000235:genetics; D014026:Nicotiana / Q000235:genetics*; D014158:Transcription, Genetic
D018075:RNA, Complementary; D012333:RNA, Messenger; D012313:RNA