title: Remarks on small sample size taken from reference population for examination of factor VIII.
abstract: Investigation of plasma factor VIII activities in 20 reference (normal) individuals (N = 20) is frequently used to evaluate the reference distribution parameters by means of simple statistical method. Two examples demonstrate the inaccuracy of conclusions of such a small sample of data. The first one shows the comparison of estimate of F.VIII:C reference limits obtained from examination of 6 different groups randomly chosen (N = 20 each) with a group of N = 120 reference individuals. The second example presents the balance of haemophilia A carrier detection rate performed in 30 obligatory carriers and 42 normal women by examination of F.VIII:C and VWF-Ag using universal discriminant. The correction figures typical for specific conditions of our laboratory are obtained from examination of N = 20 and N = 80 individuals and using heuristic optimalization.
authors: Institute of Haematology and Blood Transfusion, Praha, CSSR.
authors: J
authors: J
authors: Sikorová
authors: J
authors: J
authors: Zvárová
mesh_terms: D000293:Adolescent; D000328:Adult; D003627:Data Interpretation, Statistical; D005169:Factor VIII / Q000032:analysis*; D005260:Female; D006580:Genetic Carrier Screening / Q000379:methods; D006467:Hemophilia A / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D012016:Reference Values
chemical_list: D005169:Factor VIII