Autoradiographic localization of binding sites for galanin and VIP in small intestine.
The distribution of the binding sites for [125I]galanin and [125I]iodotyrosyl VIP in sections of small intestine from rat, rabbit, guinea pig and man was investigated using in vitro receptor autoradiography. The specific binding sites for [125I]galanin were most concentrated in the myenteric plexus and longitudinal muscle layers. The distribution of [125I]VIP binding was similar, with the highest level of binding in the myenteric plexus. Some VIP binding was also seen in the mucosa. The abundance of binding sites for both peptides in the myenteric plexus suggests that this is the anatomical site of the functional antagonism between these endogenous peptides in the mammalian intestine.
King
S C
SC
Department of Physiological Sciences, University of Manchester, U.K.
Slater
P
P
Turnberg
L A
LA
D000818:Animals; D001345:Autoradiography; D019004:Galanin; D006168:Guinea Pigs; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D007421:Intestine, Small / Q000378:metabolism*; D007457:Iodine Radioisotopes; D009130:Muscle, Smooth / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D044088:Receptors, Galanin; D011964:Receptors, Gastrointestinal Hormone / Q000378:metabolism*; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
D007457:Iodine Radioisotopes; D009479:Neuropeptides; D010455:Peptides; D044088:Receptors, Galanin; D011964:Receptors, Gastrointestinal Hormone; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide; D019004:Galanin