Substance P and gastrin releasing peptide in bovine mesenteric lymphatic vessels: chemical characterization and action.
Alcoholic extracts of bovine mesenteric lymphatic vessels were assayed for the presence of SP, GRP, VIP, PHI, GIP and NT using specific radioimmunoassays. SP and GRP immunoreactivities were detected at concentrations of 190 +/- 20 and 1,000 +/- 130 pg.g-1, respectively. No significant levels of immunoreactivity were detected for any of the other peptides. SP and GRP immunoreactivities coeluted with their synthetic counterparts from both Sephadex G-50 and reversed phase HPLC columns. Synthetic SP (10(-9)-10(-7) M) and the naturally occurring analogue of GRP, bombesin (10(-9)-10(-7) M), increased spontaneous contraction rate in isolated vessel segments. This excitatory effect was not blocked by the alpha-adrenoceptor antagonist phentolamine (3 x 10(-6) M).
Foy
W L
WL
Department of Medicine, Queens University of Belfast, Ireland, U.K.
Allen
J M
JM
McKillop
J M
JM
Goldsmith
J P
JP
Johnston
C F
CF
Buchanan
K D
KD
D000818:Animals; D001839:Bombesin / Q000494:pharmacology; D002417:Cattle; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D019886:Gastrin-Releasing Peptide; D005768:Gastrointestinal Hormones / Q000032:analysis*; D066298:In Vitro Techniques; D008208:Lymphatic System / Q000032:analysis* / Q000187:drug effects / Q000502:physiology; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000032:analysis / Q000187:drug effects / Q000502:physiology; D010455:Peptides / Q000032:analysis*; D010646:Phentolamine / Q000494:pharmacology; D011863:Radioimmunoassay; D013373:Substance P / Q000032:analysis* / Q000494:pharmacology
D005768:Gastrointestinal Hormones; D010455:Peptides; D013373:Substance P; D019886:Gastrin-Releasing Peptide; D001839:Bombesin; D010646:Phentolamine