title: Detection of granulocyte colony-stimulating factor produced by a newly established human hepatoma cell line using a simple bioassay system.
abstract: A colony-stimulating factor(CSF)-producing tumor cell line (KX-87) was established from a patient with hepatocellular carcinoma and marked granulocytosis. This cell line formed tumors on nude mice in high frequency and the mice revealed marked granulocytosis. In clonogenic assays of human bone marrow cells, KX-87 conditioned medium (CM) supported the formation of colonies mainly consisting of neutrophilic granulocytes but had no burst-promoting activity. The molecular weight of the colony-stimulating activity (CSA) in KX-87CM was estimated about 25,000 daltons by gel filtration and a new bioassay system. In principle, a subline of murine hemopoietic cell line NFS-60 was cloned which was dependent on KX-87CM. Then the growth of this subline was examined by a rapid and sensitive colorimetric tetrazolium assay. From these results, it was concluded that the CSA which KX-87 cell line produced was G-CSF.
authors: First Division of Internal Medicine, Faculty of Medicine, Kyoto University.
authors: K
authors: K
authors: Tohyama
authors: Y
authors: Y
authors: Yoshida
authors: A
authors: A
authors: Kubo
authors: T
authors: T
authors: Sudo
authors: M
authors: M
authors: Moriyama
authors: H
authors: H
authors: Sato
authors: H
authors: H
authors: Uchino
mesh_terms: D000818:Animals; D001681:Biological Assay; D001854:Bone Marrow Cells; D006528:Carcinoma, Hepatocellular / Q000032:analysis* / Q000276:immunology / Q000473:pathology; D002455:Cell Division; D003115:Colony-Stimulating Factors / Q000032:analysis*; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor; D006801:Humans; D008113:Liver Neoplasms / Q000032:analysis* / Q000276:immunology / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D008970:Molecular Weight; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured
chemical_list: D003115:Colony-Stimulating Factors; D016179:Granulocyte Colony-Stimulating Factor