Differential stability of human interleukin 1 beta fragments expressed in yeast.
We have made deletions in a human interleukin 1 beta cDNA cloned in a yeast vector capable of directing the expression and secretion of foreign protein. Deletions at either the 5' or 3' end of the cDNA result in a dramatic drop in yield of protein. An investigation of some possible causes points to instability of the truncated peptides as a major factor contributing to low yield. The results suggest that correct folding of mature interleukin 1 into a protease resistant form requires almost the entire polypeptide.
Baldari
C
C
Sclavo Research Center, Siena, Italy.
Massone
A
A
Macchia
G
G
Telford
J L
JL
D001483:Base Sequence; D002872:Chromosome Deletion; D005786:Gene Expression Regulation; D005822:Genetic Vectors; D007375:Interleukin-1 / Q000235:genetics*; D009154:Mutation; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000032:analysis / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000235:genetics
D007375:Interleukin-1; D012313:RNA