title: Effect of poly-basic amino acids on the phosphorylation of various substrate proteins by cytosolic protein-tyrosine kinase from porcine spleen.
abstract: Cytosolic protein-tyrosine kinase from porcine spleen (CPTK-40) is strongly activated by poly-L-lysine using bovine serum albumin, ovalbumin, phosphorylase b, calmodulin and H1 histone as substrate proteins. However, this polyamine inhibited the enzyme activities when myelin basic protein, tubulin and H2B histone were used as substrate proteins. These stimulatory and inhibitory effects on CPTK-40 are not specific for polylysine, but polyarginine and polyornithine have similar effects on this phosphorylation reaction. Effect of poly-basic amino acids on CPTK-40 seems to be mainly on the substrate proteins, rather than on the enzyme itself.
authors: Department of Biochemistry, Fukui Medical School, Japan.
authors: K
authors: K
authors: Sakai
authors: M
authors: M
authors: Asahi
authors: T
authors: T
authors: Kobayashi
authors: Y
authors: Y
authors: Tanaka
authors: T
authors: T
authors: Inazu
authors: S
authors: S
authors: Nakamura
authors: H
authors: H
authors: Yamamura
mesh_terms: D000818:Animals; D002147:Calmodulin / Q000378:metabolism; D002364:Caseins / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology*; D004789:Enzyme Activation / Q000187:drug effects; D006657:Histones / Q000378:metabolism; D004676:Myelin Basic Protein / Q000378:metabolism; D010047:Ovalbumin / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology; D010750:Phosphoproteins / Q000378:metabolism*; D010763:Phosphorylase b / Q000378:metabolism; D010766:Phosphorylation; D011107:Polylysine / Q000494:pharmacology*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013154:Spleen / Q000201:enzymology*; D013552:Swine; D014404:Tubulin / Q000378:metabolism
chemical_list: D002147:Calmodulin; D002364:Caseins; D006657:Histones; D004676:Myelin Basic Protein; D010455:Peptides; D010750:Phosphoproteins; D014404:Tubulin; C008973:polyornithine; D011107:Polylysine; C015462:polyarginine; D012710:Serum Albumin, Bovine; D010047:Ovalbumin; D010763:Phosphorylase b; D011505:Protein-Tyrosine Kinases