[RNA synthesis using T7 phage RNA polymerase: transcription of synthetic DNA templates in solution and on polymer support].
Transcription of synthetic DNA by T7 RNA polymerase was used to obtain oligoribonucleotides of defined sequence. The enzyme's ability to transcribe DNA immobilized on hydrazide-sepharose was revealed. DNA templates used in such synthesis can be constructed by means of enzymatic (DNA ligase) or chemical ligation (cyanogen bromide).
Elov
A A
AA
Volkov
E M
EM
Reĭntamm
T G
TG
Oretskaia
T S
TS
Shabarova
Z A
ZA
D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D008969:Molecular Sequence Data; D009843:Oligoribonucleotides / Q000096:biosynthesis; D012313:RNA / Q000096:biosynthesis*; D012685:Sepharose; D013604:T-Phages / Q000201:enzymology*; D013698:Templates, Genetic; D014158:Transcription, Genetic
D009843:Oligoribonucleotides; D012313:RNA; D004247:DNA; D012685:Sepharose; D012321:DNA-Directed RNA Polymerases
Sintez RNK s pomoshch'iu RNK-polimerazy faga T7: transkriptsiia sinteticheskikh DNK-matrits v rastvore i na polimernom nositele.