Analysis of neurotransmitter metabolite concentrations in canine cerebrospinal fluid.
The concentrations of dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), and 5-hydroxyindole-3-acetic acid (5-HIAA) in CSF obtained from the cisterna magna of 21 nonneurologically compromised dogs were determined by high-pressure liquid chromatography and electrochemical detection. A rapid method of sample preparation, which involved single filtration through a deproteinizing membrane, was used. Canine CSF obtained in this manner contained 5.78 +/- 0.78 ng of DOPAC/ml, 72.19 +/- 4.09 ng of HVA/ml, and 29.95 +/- 1.67 ng of 5-HIAA/ml. Linear regression analysis between HVA and 5-HIAA yielded a correlation coefficient of 0.4804. The neurotransmitter index, HVA/5-HIAA, was found to be more indicative of the dopaminergic metabolite HVA than the acid metabolite of serotonin, 5-HIAA (correlation coefficient with HVA = 0.5529 vs a correlation coefficient with 5-HIAA = -0.4462). A poor relationship (correlation coefficient = -0.1715) was found to exist between the 2 dopaminergic metabolites DOPAC and HVA in the CSF.
Vaughn
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Medicinal Biochemistry Laboratory, Scott-Ritchey Research Program, Auburn University, AL 36849.
Coleman
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Simpson
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Satjawatcharaphong
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D015102:3,4-Dihydroxyphenylacetic Acid / Q000134:cerebrospinal fluid*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004285:Dogs / Q000134:cerebrospinal fluid*; D004298:Dopamine / Q000134:cerebrospinal fluid; D004837:Epinephrine / Q000134:cerebrospinal fluid; D005260:Female; D006719:Homovanillic Acid / Q000134:cerebrospinal fluid*; D006897:Hydroxyindoleacetic Acid / Q000134:cerebrospinal fluid*; D008297:Male; D009638:Norepinephrine / Q000134:cerebrospinal fluid; D010648:Phenylacetates / Q000134:cerebrospinal fluid*; D012701:Serotonin / Q000134:cerebrospinal fluid
D010648:Phenylacetates; D015102:3,4-Dihydroxyphenylacetic Acid; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D004298:Dopamine; D009638:Norepinephrine; D006719:Homovanillic Acid; D004837:Epinephrine