[Experimental studies of 9-(1, 3-dihydroxy-2-propoxymethyl)-guanine(DHPG) against herpes simplex virus].
The antiherpes virus effects of 9-(1,3-dihydroxy-2-propoxymethyl) guanine (DHPG) were studied in cell culture and in experimental herpetic keratitis in rabbits. In plaque reduction assays in Vero cell, the 50% inhibitory concentration (IC50) of DHPG was shown to be 0.1 microgram/ml against HSV-1. Combination of DHPG and Cyclocytidine (CC), produced a synergistic activity against kos strains of HSV-1. Whereas the combination of DHPG and Acyclovir (ACV) or Tai-Ding-An (TDA) resulted in an additive interaction against HSV-1. Topical application of 0.1% or 0.05% DHPG eyedrop was initiated 48h after virus inoculation, significant efficacy was obtained in rabbits with herpetic epithelial keratitis. But 0.025 and 0.01% DHPG eyedrop showed no effect. 0.1% DHPG has been shown to be effective in the topical treatment of herpetic stromal keratitis in the rabbits.
Chen
Z J
ZJ
Liu
W G
WG
Song
J Z
JZ
D000212:Acyclovir / Q000494:pharmacology; D003504:Ancitabine / Q000494:pharmacology; D000818:Animals; D000998:Antiviral Agents; D004357:Drug Synergism; D015774:Ganciclovir / Q000494:pharmacology* / Q000627:therapeutic use; D007635:Keratitis, Dendritic / Q000188:drug therapy; D010797:Phthalimides / Q000494:pharmacology; D011817:Rabbits; D018139:Simplexvirus / Q000187:drug effects*; D013882:Thiosemicarbazones / Q000494:pharmacology; D014709:Vero Cells
D000998:Antiviral Agents; D010797:Phthalimides; D013882:Thiosemicarbazones; D003504:Ancitabine; D015774:Ganciclovir; C031410:phtiobuzone; D000212:Acyclovir