The autoantigenic response to rabbit cytochrome c.
In this report we describe the production and characterization of autoantibody responses to rabbit cytochrome c (cyt c) in rabbits immunized with either the native monomeric or polymerized form of rabbit cyt c. Fine-specificity analyses of the response indicated that the majority of the response was to the evolutionarily conserved amino-terminal region of the molecule. The relative affinity of the autoantibody interaction with rabbit cyt c was assessed by a solid-phase assay and was found to be lower than that observed for rabbit anti-horse cyt c antibody populations. These findings are consistent with the prediction that low-affinity self-reactive B cells may escape tolerance induction.
Cooper
H M
HM
Department of Immunology, Scripps Clinic & Research Foundation, La Jolla.
Klinman
N R
NR
Paterson
Y
Y
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000276:immunology / Q000302:isolation & purification; D000818:Animals; D000915:Antibody Affinity; D000918:Antibody Specificity; D001323:Autoantibodies / Q000096:biosynthesis*; D001324:Autoantigens / Q000008:administration & dosage*; D001667:Binding, Competitive; D003574:Cytochrome c Group / Q000008:administration & dosage / Q000276:immunology*; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000008:administration & dosage / Q000276:immunology; D011817:Rabbits
D000596:Amino Acids; D001323:Autoantibodies; D001324:Autoantigens; D003574:Cytochrome c Group; D000939:Epitopes; D046911:Macromolecular Substances; D010446:Peptide Fragments