A 595 nanometer band-pass filter enhances the contrast of in situ hybridization signals on chromosome observed after biotin/avidin-alkaline phosphatase localization.
In situ hybridization with DNA probes labeled with biotin and detected by the avidin-alkaline phosphatase/5-bromo-chloroindoxyl phosphate-nitro blue tetrazolium system has been used to localize DNA sequences in chromosomes. To observe the hybridization signals, a phase contrast microscope has often been used because of the good visibility it provides. Use of a 595 nm band pass filter with the phase contrast microscope enhances signal contrast after in situ hybridization without reducing resolution.
Yasue
H
H
Department of Animal Breeding and Genetics, National Institute of Animal Industry, Ibaraki, Japan.
Awata
T
T
Muramatsu
S
S
D000469:Alkaline Phosphatase; D000818:Animals; D001360:Avidin; D001710:Biotin; D002472:Cell Transformation, Viral; D002875:Chromosomes / Q000378:metabolism* / Q000648:ultrastructure; D015342:DNA Probes; D004279:DNA, Viral / Q000378:metabolism; D008858:Microscopy, Phase-Contrast / Q000379:methods*; D009693:Nucleic Acid Hybridization; D013194:Staining and Labeling / Q000379:methods*
D015342:DNA Probes; D004279:DNA, Viral; D001360:Avidin; D001710:Biotin; D000469:Alkaline Phosphatase