Putative chicken "muscle-specific 7 S RNA" is related to the mitochondrial ATPase 6 gene.
Sequence analysis of the mitochondrial ATPase 6 gene from chicken revealed that its 3' region is virtually identical with a chicken muscle-specific 7 S RNA which was reported to induce the expression of tissue-specific functions in blastoderm explants. Using chicken and quail cell lines depleted of mitochondrial DNA, we demonstrate that the 7 S RNA is encoded by the mitochondrial genome and not by nuclear (repetitive) DNA as suggested previously. Moreover, no 7 S RNA-homologous transcript of the expected length (about 400 bases) is detected, either in these cell lines or in heart and liver tissues. The only RNA species hybridizing with a 7 S RNA-specific probe is an abundant, 900 base long transcript of mitochondrial origin that we identify as the ATPase 8-ATPase 6 fused messenger. We suggest that the characterized muscle-specific 7 S RNA cDNA is derived from an unrelated contaminant in the blastoderm-inducing fraction.
Desjardins
P
P
Département de biochimie, Faculté de médecine, Université de Montréal, Québec, Canada.
L'Abbé
D
D
Lang
B F
BF
Morais
R
R
D000251:Adenosine Triphosphatases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002645:Chickens / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005796:Genes; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics*
D012313:RNA; D004247:DNA; D000251:Adenosine Triphosphatases