A region of the influenza A/NT/60/68 PB2 protein containing an antigenic determinant recognized by murine H-2Dd restricted cytotoxic T lymphocytes.
We have used a recombinant vaccinia virus to investigate the recognition of the PB2 protein of influenza A/NT/60/68 (H3N2) by murine polyclonal CTL populations. PB2 is recognized as a major cross-reactive target antigen. Recognition of PB2 is under strict genetic control, since BALB/c (H-2d) but not CBA (H-2k) mice are responders. We also demonstrate, by use of cell lines transfected with individual genes encoding class I molecules of the H-2d haplotype, that recognition of PB2 occurs in conjunction with the H-2Dd but not the H-2Kd or H-2Ld molecules. In contrast, recognition of the nucleoprotein of A/PR/8/34 by BALB/c-derived polyclonal CTL is restricted via the H-2Kd molecule. By using three recombinant vaccinia viruses expressing deleted forms of the PB2 protein we show that at least one epitope of the PB2 protein resides within the amino-terminal 256 amino acids. This approach offers an effective method to map the regions of large proteins containing epitopes recognized by CTL.
Reay
P A
PA
Sir William Dunn School of Pathology, University of Oxford, United Kingdom.
Jones
I M
IM
Brownlee
G G
GG
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D015153:Blotting, Western; D002460:Cell Line; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D005260:Female; D005786:Gene Expression Regulation; D006183:H-2 Antigens; D009980:Influenza A virus / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D010452:Peptide Biosynthesis; D010455:Peptides / Q000235:genetics; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014162:Transfection; D014616:Vaccinia virus / Q000235:genetics; D014764:Viral Proteins / Q000276:immunology*
D000956:Antigens, Viral; D000939:Epitopes; D006183:H-2 Antigens; D010455:Peptides; D014764:Viral Proteins