Identification of amino acids relevant to three antigenic determinants on the fusion protein of Newcastle disease virus that are involved in fusion inhibition and neutralization.
Nucleotide sequence analysis of F protein antigenic variants of Newcastle disease virus mapped three distinct antigenic determinants to positions 343, 72, and 161 on the protein. The high fusion-inhibiting and neutralizing capacities of all of the monoclonal antibodies used for selection suggested close functional and structural relationships of the three positions with the fusion-inducing N-terminal region of the F1 subunit. The former two positions were located at the cysteine cluster domain near the C terminus of the F1 subunit and at the major hydrophilic domain in the F2 subunit, respectively, and both domains appeared to represent the major antigenic determinants of paramyxovirus F protein.
Toyoda
T
T
Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Japan.
Gotoh
B
B
Sakaguchi
T
T
Kida
H
H
Nagai
Y
Y
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D000998:Antiviral Agents; D000939:Epitopes; D008969:Molecular Sequence Data; D009522:Newcastle disease virus / Q000276:immunology*; D014760:Viral Fusion Proteins / Q000276:immunology*
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D000998:Antiviral Agents; D000939:Epitopes; D014760:Viral Fusion Proteins