title: Polymerization of turtle alpha-macroglobulin through newly exposed sulfhydryls reveals the location of ex-thiolester bonds.
abstract: Green turtle alpha-macroglobulin, which has previously been shown to contain thiolester bonds, formed linear polymers after being treated with proteinases. Biochemical analyses showed that the polymerization proceeded through disulfide-bond formation between monomers. The only sulfhydryl groups available for such polymerization after proteinase treatment were those created as the product of thiolester hydrolysis. Electron micrographs of polymers revealed H-shaped monomeric units aligned lengthwise in linear polymers. The average length per monomeric unit in the polymer estimated from the discrete distribution of polymer lengths was approximately 80% of the average length of free monomers, indicating that monomers overlapped each other within a region of about 4 nm. From such observations we concluded that the newly produced sulfhydryl groups were located on the four arms of the H-shaped molecule. The location of sulfhydryls can be taken as the site of the exposure of thiolesters which were originally sequestered in the hydrophobic interior of the molecule. Since the structure of turtle alpha-macroglobulin is very similar to that of human serum alpha 2-macroglobulin the results predict a similar location of sulfhydryls in human alpha 2-macroglobulin after proteinase treatment. The observed polymerization property is unique to sea turtle alpha-macroglobulin and has not been observed with human alpha 2-macroglobulin or other homologous proteins.
authors: Department of Biochemistry and Biophysics, Faculty of Science, University of Tokyo, Japan.
authors: T
authors: T
authors: Osada
authors: M
authors: M
authors: Nishigai
authors: A
authors: A
authors: Ikai
mesh_terms: D000818:Animals; D008854:Microscopy, Electron; D011108:Polymers; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D014426:Turtles / Q000378:metabolism*; D000511:alpha-Macroglobulins / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D011108:Polymers; D013438:Sulfhydryl Compounds; D000511:alpha-Macroglobulins