Influence of Bay k 8644 on aequorin-loaded human platelets.
In aequorin-loaded human platelets, Bay k 8644 (1-100 microM) had no effect on the resting level of cytoplasmic Ca2+ and did not induce aggregation by itself. Though the rise of cytoplasmic Ca2+ and platelet aggregation induced by thrombin (0.1 U/ml) also was not inhibited by 1-10 microM of Bay k 8644, at concentration of 3-10 microM, it dose-dependently reduced those induced by collagen (5 micrograms/ml). The present study shows that human platelets do not have dihydropyridine sensitive Ca channels.
Ogawa
N
N
Department of Pharmacology and Toxicology, Hatano Research Institute, Food and Drug Safety Center, Kanagawa, Japan.
Ono
H
H
D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / Q000494:pharmacology*; D000331:Aequorin / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D002118:Calcium / Q000097:blood; D003593:Cytoplasm / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008164:Luminescent Proteins / Q000494:pharmacology*; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects
D008164:Luminescent Proteins; D000331:Aequorin; D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; D002118:Calcium