[Postoperative, intra-abdominal adhesions--a new standardized and objective animal model and testing of substances for the prevention of adhesions].
80 male Sprague Dawley rats were divided in 8 groups of equal size. After median laparotomy defined abrasions of the serosa of abdominal wall, cecum and ileum were performed (400p, abrasive paper 280 grains/cm2, 8 cm2). The control group (I) received no medication. In the treatment groups 5 ml of the following agents were instilled before closure of the abdominal wall: normal saline (II), 16,250 IE Neomycin with 1250 IE Bacitracin (III), 30% dextrose (MW 70,000) (IV), 50,000 IE Streptokinase with 12,500 IE Streptodornase (V), or TCDO in concentrations of 10% (VI), 50% (VII), and 100% (VIII). All animals were relaparotomized on the 7th postoperative day, the adhesions were dissected and their extent was calculated by computer aid. Furthermore specimens were obtained for microscopic studies. Compared to the controls no reduction of adhesions could be achieved by dextrose. Significantly more adhesions were observed after treatment with Neomycin/Bacitracin. A reduction of about 23% was registered with normal saline and TCDO 10%. The greatest reduction of adhesions was seen after application of Streptokinase/Streptodornase as well as TCDO in concentrations of 50% and 100% (63%). The results were significant after evaluation with the t-test. Histologically there was a correlation between the extent of adhesions and the fibrin film. The new animal model has proven to result in reproducible data if used to evaluate substances for prevention of adhesions. Clinical studies with the best of these agents could serve as an approach to solve the problem of adhesion ileus.
Treutner
K H
KH
Chirurgische Klinik, Medizinischen Fakultät, Rheinisch-Westfalischen Technischen Hochschule Aachen.
Winkeltau
G
G
Lerch
M M
MM
Stadel
R
R
Schumpelick
V
V
D000005:Abdomen / Q000601:surgery*; D000818:Animals; D001414:Bacitracin / Q000008:administration & dosage; D002713:Chlorine / Q000008:administration & dosage; D003911:Dextrans / Q000008:administration & dosage; D007274:Injections, Intraperitoneal; D008297:Male; D009355:Neomycin / Q000008:administration & dosage; D010087:Oxides / Q000008:administration & dosage; D011183:Postoperative Complications / Q000517:prevention & control*; D051381:Rats; D011919:Rats, Inbred Strains; D012965:Sodium Chloride / Q000008:administration & dosage; D013299:Streptodornase and Streptokinase / Q000008:administration & dosage; D000267:Tissue Adhesions
D003911:Dextrans; D010087:Oxides; D001414:Bacitracin; D012965:Sodium Chloride; D002713:Chlorine; C041955:tetrachlorodecaoxide; D013299:Streptodornase and Streptokinase; D009355:Neomycin
Postoperative, intraabdominelle Adhäsionen--Ein neues standardisiertes und objektiviertes Tiermodell und Testung von Substanzen zur Adhäsionsprophylaxe.