title: Assessment of the Dutch organ-culture system of corneal preservation within the Eye Bank of South Australia.
abstract: Thirteen per cent of all corneas harvested by the Eye Bank of South Australia during 1986 were discarded because storage time in McCarey-Kaufman medium exceeded four days. We have therefore examined the suitability of the Dutch method of long-term corneal storage for our purposes. Twenty-two human corneas that had been discarded from the Eye Bank were assessed using the trypan blue-sucrose staining technique, and then placed into long-term storage for 15 to 17 days. They were then reassessed by vital dye staining before permanent flat-mounts were prepared for silver staining of the endothelium. A good correlation (albeit subjective) was found between the non-destructive and destructive techniques of endothelial cell assessment. Those corneas that failed to survive organ culture storage were easily detected. The Dutch system of corneal preservation and post-storage assessment seems well-suited to Australian eye-banking.
authors: Department of Ophthalmology, Flinders Medical Centre, Bedford Park, South Australia.
authors: K A
authors: KA
authors: Williams
authors: L M
authors: LM
authors: Noack
authors: S J
authors: SJ
authors: Alfrich
authors: R
authors: R
authors: Danz
authors: S A
authors: SA
authors: Erickson
authors: D J
authors: DJ
authors: Coster
mesh_terms: D003315:Cornea; D003470:Culture Media; D004728:Endothelium, Corneal / Q000473:pathology; D005125:Eye Banks; D006801:Humans; D009924:Organ Culture Techniques / Q000295:instrumentation / Q000379:methods; D009926:Organ Preservation / Q000295:instrumentation / Q000379:methods*; D012835:Silver Nitrate; D013021:South Australia; D013194:Staining and Labeling / Q000379:methods; D013395:Sucrose; D014015:Tissue Banks; D014022:Tissue Survival; D014343:Trypan Blue
chemical_list: D003470:Culture Media; D013395:Sucrose; D012835:Silver Nitrate; D014343:Trypan Blue