Can immunohistology improve detection of lymph-node metastases in large-bowel cancer?
Employing the monoclonal antibody CAM5.2, the sensitivity of immunohistologic staining was compared with conventional hematoxylin and eosin staining for detection of lymph-node metastases from large-bowel cancer. Ten patients who died unexpectedly early from recurrent disease were selected; 86 lymph nodes were examined. Where metastases were located they were readily identified by both methods but when the original lymph-node sections were reviewed, attention was drawn to two metastatic deposits that had been overlooked previously. These results suggest that immunohistologic techniques offer no advantage in the identification of lymph-node metastases in large-bowel cancer.
Makin
C A
CA
Imperial Cancer Research Fund, Lincolns Inn Fields, London, United Kingdom.
Bobrow
L G
LG
Nicholls
R J
RJ
D000911:Antibodies, Monoclonal; D003110:Colonic Neoplasms; D006801:Humans; D007124:Immunoenzyme Techniques; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis / Q000175:diagnosis* / Q000473:pathology; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal