Isolation and characterization of two fatty acid binding proteins from intestine of Gallus domesticus.
1. Two distinct fatty acid binding proteins (FABPs) were isolated and characterized from chicken duodenal mucosa. 2. Molecular weight, functional activity, immunospecificity, mRNA expression, and amino acid composition data for the 14 kDa chicken intestinal FABP was similar, yet not identical, to that of a previously isolated chicken liver FABP. 3. Bound fatty acids were shown to produce isoforms of the 14 kDa intestinal protein but not the larger molecular weight intestinal FABP.
Sewell
J E
JE
Department of Poultry Science, Texas Agricultural Experiment Station, Texas A&M University System, College Station 77843-2472.
Young
C R
CR
Davis
S K
SK
Hargis
P S
PS
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000032:analysis / Q000302:isolation & purification*; D002645:Chickens / Q000378:metabolism*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007525:Isoelectric Focusing; D012313:RNA / Q000302:isolation & purification
D000596:Amino Acids; D002352:Carrier Proteins; D005227:Fatty Acids; D012313:RNA