The human mannose-binding protein gene. Exon structure reveals its evolutionary relationship to a human pulmonary surfactant gene and localization to chromosome 10.
The human mannose-binding protein (MBP) plays a role in first line host defense against certain pathogens. It is an acute phase protein that exists in serum as a multimer of a 32-kD subunit. The NH2 terminus is rich in cysteines that mediate interchain disulphide bonds and stabilize the second collagen-like region. This is followed by a short intervening region, and the carbohydrate recognition domain is found in the COOH-terminal region. Analysis of the human MBP gene reveals that the coding region is interrupted by three introns, and all four exons appear to encode a distinct domain of the protein. It appears that the human MBP gene has evolved by recombination of an ancestral nonfibrillar collagen gene with a gene that encodes carbohydrate recognition, and is therefore similar to the human surfactant SP-A gene and the rat MBP gene. The gene for MBP is located on the long arm of chromosome 10 at 10q11.2-q21, a region that is included in the assignment for the gene for multiple endocrine neoplasia type 2A.
Sastry
K
K
Division of Hematology/Oncology, Children's Hospital, Boston, Massachusetts 02115.
Herman
G A
GA
Day
L
L
Deignan
E
E
Bruns
G
G
Morton
C C
CC
Ezekowitz
R A
RA
D000209:Acute-Phase Proteins / Q000235:genetics* / Q000648:ultrastructure; D000595:Amino Acid Sequence; D001483:Base Sequence; D005075:Biological Evolution; D015139:Blotting, Southern; D002352:Carrier Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D005091:Exons; D006801:Humans; D037241:Mannose-Binding Lectins; D008969:Molecular Sequence Data; D011663:Pulmonary Surfactants / Q000235:genetics*; D015183:Restriction Mapping; D013329:Structure-Activity Relationship
D000209:Acute-Phase Proteins; D002352:Carrier Proteins; D037241:Mannose-Binding Lectins; D011663:Pulmonary Surfactants