Ca2+ influx in normal and spherocytic red cells.
The influx of 45Ca2+ into normal red cells and various types of spherocytic red cells was studied after blocking active Ca2+ extrusion by vanadate. The measurements were performed with and without verapamil, a calcium antagonist. The influx of Ca2+ into red cells from unsplenectomized persons was 22 +/- 7 mumol/l packed red cells/h (mean +/- SD), and 17 +/- 7 mumol/l per h when incubated with verapamil. The influx of Ca2+ into red cells from four splenectomized normal controls was of the same magnitude as in the unsplenectomized controls but there was no effect of verapamil on the influx rate. The influx of Ca2+ into red cells from nine splenectomized patients with hereditary spherocytosis (HS) was 27 +/- 9 mumol/l per h without and 24 +/- 9 mumol/l per h with verapamil. In 9 normal red cell samples made spherocytic by thermal damage the corresponding values were 32 +/- 16 and 31 +/- 19 mumol/l per h, respectively. The uptake of Ca2+ in chlorpromazine-induced spherocytic red cells was 20 +/- 4 mumol/l per h without and 19 +/- 5 mumol/l per h with verapamil in 9 experiments. These results indicate that although in HS erythrocytes changes in the cell membrane lead to an increased Ca2+ influx, the slow calcium channels are not affected, whereas in spherocytes induced by thermal damage or by incubation with chlorpromazine the channels are blocked, at least partly.
Johnsson
R
R
Laakso Hospital, City of Helsinki, Finland.
Saris
N E
NE
D000328:Adult; D002118:Calcium / Q000097:blood*; D002746:Chlorpromazine / Q000009:adverse effects; D004912:Erythrocytes / Q000378:metabolism*; D004913:Erythrocytes, Abnormal / Q000378:metabolism*; D005260:Female; D006801:Humans; D007473:Ion Channels / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D013102:Spherocytes / Q000378:metabolism*; D013103:Spherocytosis, Hereditary / Q000097:blood*; D013156:Splenectomy; D014638:Vanadates / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
D007473:Ion Channels; D014638:Vanadates; D014700:Verapamil; D002118:Calcium; D002746:Chlorpromazine