Biochemical characterization of scleroderma-inducing glycosaminoglycan.
The scleroderma-inducing N-sulfated glycosaminoglycan previously isolated by us from the urine of patients with systemic scleroderma was further purified: it was biochemically characterized by low O-sulfation and relative high N-sulfation. Consistent with this finding, desulfated and N-resulfated heparin, which had a similar composition to the urine-derived scleroderma-inducing glycosaminoglycan, induced a significant degree of sclerotic fibrosis in the skin of mice which had received intraperitoneal injections of it, whereas N-desulfated heparin with contrasting sulfation to it failed to cause any significant change in the skin.
Ishikawa
H
H
Department of Dermatology, Gunma University School of Medicine, Maebashi, Japan.
Kitabatake
M
M
Akiyama
F
F
D000818:Animals; D006025:Glycosaminoglycans / Q000032:analysis* / Q000633:toxicity / Q000652:urine; D006493:Heparin; D006801:Humans; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012595:Scleroderma, Systemic / Q000139:chemically induced / Q000473:pathology* / Q000652:urine; D012867:Skin / Q000473:pathology; D013431:Sulfates
D006025:Glycosaminoglycans; D013431:Sulfates; D006493:Heparin