Alcian blue staining of proteoglycans in polyacrylamide gels using the "critical electrolyte concentration" approach.
A method is described here for Alcian blue staining of proteoglycans in polyacrylamide gels; this is illustrated using extracts obtained from bovine corneal stroma. Other available methods for visualization of proteoglycans can produce nonspecific staining and frequently a high background in the gel; with a "critical electrolyte concentration" approach, specific staining against a clear background can be obtained.
Wall
R S
RS
Open University, Oxford Research Unit, United Kingdom.
Gyi
T J
TJ
D000423:Alcian Blue; D004591:Electrophoresis, Polyacrylamide Gel; D011509:Proteoglycans / Q000302:isolation & purification*; D013194:Staining and Labeling / Q000379:methods*
D011509:Proteoglycans; D000423:Alcian Blue